LOCUS       DAPQZD010000001       113866 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPQZD010000001 DAPQZD010000000
VERSION     DAPQZD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 113866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.001"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            38..514
                     /gene="agaB"
                     /locus_tag="RO454_000001"
     CDS             38..514
                     /gene="agaB"
                     /locus_tag="RO454_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDY0931896.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            553..1356
                     /gene="agaC"
                     /locus_tag="RO454_000002"
     CDS             553..1356
                     /gene="agaC"
                     /locus_tag="RO454_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDY0931897.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1346..2137
                     /gene="agaD"
                     /locus_tag="RO454_000003"
     CDS             1346..2137
                     /gene="agaD"
                     /locus_tag="RO454_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDY0931898.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKNDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2138..2905
                     /locus_tag="RO454_000004"
     CDS             2138..2905
                     /locus_tag="RO454_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDY0931899.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(2955..3818)
                     /gene="rsmI"
                     /locus_tag="RO454_000005"
     CDS             complement(2955..3818)
                     /gene="rsmI"
                     /locus_tag="RO454_000005"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDY0931900.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3883..5919
                     /gene="lpoA"
                     /locus_tag="RO454_000006"
     CDS             3883..5919
                     /gene="lpoA"
                     /locus_tag="RO454_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDY0931901.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            5877..6272
                     /locus_tag="RO454_000007"
     CDS             5877..6272
                     /locus_tag="RO454_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDY0931902.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6292..6882
                     /gene="diaA"
                     /locus_tag="RO454_000008"
     CDS             6292..6882
                     /gene="diaA"
                     /locus_tag="RO454_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDY0931903.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6892..7467
                     /gene="yraP"
                     /locus_tag="RO454_000009"
     CDS             6892..7467
                     /gene="yraP"
                     /locus_tag="RO454_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDY0931904.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7580..8620)
                     /locus_tag="RO454_000010"
     CDS             complement(7580..8620)
                     /locus_tag="RO454_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDY0931905.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8693..9328)
                     /locus_tag="RO454_000011"
     CDS             complement(8693..9328)
                     /locus_tag="RO454_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDY0931906.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9456..9974
                     /gene="yhbO"
                     /locus_tag="RO454_000012"
     CDS             9456..9974
                     /gene="yhbO"
                     /locus_tag="RO454_000012"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDY0931907.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9954..10397)
                     /locus_tag="RO454_000013"
     CDS             complement(9954..10397)
                     /locus_tag="RO454_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDY0931908.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10448..10750
                     /gene="yhbQ"
                     /locus_tag="RO454_000014"
     CDS             10448..10750
                     /gene="yhbQ"
                     /locus_tag="RO454_000014"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDY0931909.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQTPEIKSD"
     gene            complement(10737..11240)
                     /locus_tag="RO454_000015"
     CDS             complement(10737..11240)
                     /locus_tag="RO454_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0931910.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11234..11758)
                     /locus_tag="RO454_000016"
     CDS             complement(11234..11758)
                     /locus_tag="RO454_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDY0931911.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11967..12962
                     /locus_tag="RO454_000017"
     CDS             11967..12962
                     /locus_tag="RO454_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDY0931912.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12971..13849
                     /locus_tag="RO454_000018"
     CDS             12971..13849
                     /locus_tag="RO454_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDY0931913.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13930..14937
                     /locus_tag="RO454_000019"
     CDS             13930..14937
                     /locus_tag="RO454_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDY0931914.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15055..16299)
                     /gene="mtr"
                     /locus_tag="RO454_000020"
     CDS             complement(15055..16299)
                     /gene="mtr"
                     /locus_tag="RO454_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDY0931915.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16453..18342)
                     /gene="deaD"
                     /locus_tag="RO454_000021"
     CDS             complement(16453..18342)
                     /gene="deaD"
                     /locus_tag="RO454_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDY0931916.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18335..18415)
                     /gene="yrbN"
                     /locus_tag="RO454_000022"
     CDS             complement(18335..18415)
                     /gene="yrbN"
                     /locus_tag="RO454_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDY0931917.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18522..19406)
                     /gene="nlpI"
                     /locus_tag="RO454_000023"
     CDS             complement(18522..19406)
                     /gene="nlpI"
                     /locus_tag="RO454_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDY0931918.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19515..21650)
                     /gene="pnp"
                     /locus_tag="RO454_000024"
     CDS             complement(19515..21650)
                     /gene="pnp"
                     /locus_tag="RO454_000024"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDY0931919.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21897..22166)
                     /gene="rpsO"
                     /locus_tag="RO454_000025"
     CDS             complement(21897..22166)
                     /gene="rpsO"
                     /locus_tag="RO454_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDY0931920.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22315..23259)
                     /gene="truB"
                     /locus_tag="RO454_000026"
     CDS             complement(22315..23259)
                     /gene="truB"
                     /locus_tag="RO454_000026"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDY0931921.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23259..23660)
                     /gene="rbfA"
                     /locus_tag="RO454_000027"
     CDS             complement(23259..23660)
                     /gene="rbfA"
                     /locus_tag="RO454_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDY0931922.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23824..26496)
                     /gene="infB"
                     /locus_tag="RO454_000028"
     CDS             complement(23824..26496)
                     /gene="infB"
                     /locus_tag="RO454_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDY0931923.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26521..28008)
                     /gene="nusA"
                     /locus_tag="RO454_000029"
     CDS             complement(26521..28008)
                     /gene="nusA"
                     /locus_tag="RO454_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDY0931924.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28036..28488)
                     /gene="rimP"
                     /locus_tag="RO454_000030"
     CDS             complement(28036..28488)
                     /gene="rimP"
                     /locus_tag="RO454_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDY0931925.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28695..28771)
                     /locus_tag="RO454_000031"
     tRNA            complement(28695..28771)
                     /locus_tag="RO454_000031"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28735..28737),aa:Met,seq:cat)
     gene            29119..30462
                     /gene="argG"
                     /locus_tag="RO454_000032"
     CDS             29119..30462
                     /gene="argG"
                     /locus_tag="RO454_000032"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDY0931926.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30470..32095)
                     /locus_tag="RO454_000033"
     CDS             complement(30470..32095)
                     /locus_tag="RO454_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDY0931927.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIIFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(32552..32638)
                     /locus_tag="RO454_000034"
     tRNA            complement(32552..32638)
                     /locus_tag="RO454_000034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32602..32604),aa:Leu,seq:gag)
     gene            complement(32653..32985)
                     /gene="secG"
                     /locus_tag="RO454_000035"
     CDS             complement(32653..32985)
                     /gene="secG"
                     /locus_tag="RO454_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDY0931928.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33213..34550)
                     /gene="glmM"
                     /locus_tag="RO454_000036"
     CDS             complement(33213..34550)
                     /gene="glmM"
                     /locus_tag="RO454_000036"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDY0931929.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34543..35391)
                     /gene="folP"
                     /locus_tag="RO454_000037"
     CDS             complement(34543..35391)
                     /gene="folP"
                     /locus_tag="RO454_000037"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDY0931930.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35481..37415)
                     /gene="ftsH"
                     /locus_tag="RO454_000038"
     CDS             complement(35481..37415)
                     /gene="ftsH"
                     /locus_tag="RO454_000038"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDY0931931.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37515..38144)
                     /gene="rlmE"
                     /locus_tag="RO454_000039"
     CDS             complement(37515..38144)
                     /gene="rlmE"
                     /locus_tag="RO454_000039"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDY0931932.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38270..38563
                     /gene="yhbY"
                     /locus_tag="RO454_000040"
     CDS             38270..38563
                     /gene="yhbY"
                     /locus_tag="RO454_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDY0931933.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38719..39195)
                     /gene="greA"
                     /locus_tag="RO454_000041"
     CDS             complement(38719..39195)
                     /gene="greA"
                     /locus_tag="RO454_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDY0931934.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39443..40876
                     /gene="dacB"
                     /locus_tag="RO454_000042"
     CDS             39443..40876
                     /gene="dacB"
                     /locus_tag="RO454_000042"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDY0931935.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40917..42089)
                     /gene="cgtA"
                     /locus_tag="RO454_000043"
     CDS             complement(40917..42089)
                     /gene="cgtA"
                     /locus_tag="RO454_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDY0931936.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42105..43070)
                     /locus_tag="RO454_000044"
     CDS             complement(42105..43070)
                     /locus_tag="RO454_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDY0931937.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43197..43454)
                     /gene="rpmA"
                     /locus_tag="RO454_000045"
     CDS             complement(43197..43454)
                     /gene="rpmA"
                     /locus_tag="RO454_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDY0931938.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43475..43786)
                     /gene="rplU"
                     /locus_tag="RO454_000046"
     CDS             complement(43475..43786)
                     /gene="rplU"
                     /locus_tag="RO454_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDY0931939.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44045..45016
                     /gene="ispB"
                     /locus_tag="RO454_000047"
     CDS             44045..45016
                     /gene="ispB"
                     /locus_tag="RO454_000047"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDY0931940.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45249..45527
                     /gene="sfsB"
                     /locus_tag="RO454_000048"
     CDS             45249..45527
                     /gene="sfsB"
                     /locus_tag="RO454_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDY0931941.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45575..46834)
                     /gene="murA"
                     /locus_tag="RO454_000049"
     CDS             complement(45575..46834)
                     /gene="murA"
                     /locus_tag="RO454_000049"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDY0931942.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46889..47143)
                     /gene="ibaG"
                     /locus_tag="RO454_000050"
     CDS             complement(46889..47143)
                     /gene="ibaG"
                     /locus_tag="RO454_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDY0931943.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47303..47596)
                     /gene="mlaB"
                     /locus_tag="RO454_000051"
     CDS             complement(47303..47596)
                     /gene="mlaB"
                     /locus_tag="RO454_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDY0931944.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47596..48231)
                     /gene="mlaC"
                     /locus_tag="RO454_000052"
     CDS             complement(47596..48231)
                     /gene="mlaC"
                     /locus_tag="RO454_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDY0931945.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48250..48801)
                     /gene="mlaD"
                     /locus_tag="RO454_000053"
     CDS             complement(48250..48801)
                     /gene="mlaD"
                     /locus_tag="RO454_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDY0931946.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48806..49588)
                     /gene="mlaE"
                     /locus_tag="RO454_000054"
     CDS             complement(48806..49588)
                     /gene="mlaE"
                     /locus_tag="RO454_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDY0931947.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49596..50405)
                     /gene="mlaF"
                     /locus_tag="RO454_000055"
     CDS             complement(49596..50405)
                     /gene="mlaF"
                     /locus_tag="RO454_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDY0931948.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50615..51592
                     /locus_tag="RO454_000056"
     CDS             50615..51592
                     /locus_tag="RO454_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDY0931949.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51606..52592
                     /gene="kdsD"
                     /locus_tag="RO454_000057"
     CDS             51606..52592
                     /gene="kdsD"
                     /locus_tag="RO454_000057"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDY0931950.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52613..53179
                     /gene="kdsC"
                     /locus_tag="RO454_000058"
     CDS             52613..53179
                     /gene="kdsC"
                     /locus_tag="RO454_000058"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDY0931951.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53176..53751
                     /gene="lptC"
                     /locus_tag="RO454_000059"
     CDS             53176..53751
                     /gene="lptC"
                     /locus_tag="RO454_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDY0931952.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53720..54277
                     /gene="lptA"
                     /locus_tag="RO454_000060"
     CDS             53720..54277
                     /gene="lptA"
                     /locus_tag="RO454_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDY0931953.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54284..55009
                     /gene="lptB"
                     /locus_tag="RO454_000061"
     CDS             54284..55009
                     /gene="lptB"
                     /locus_tag="RO454_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDY0931954.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55057..56490
                     /gene="rpoN"
                     /locus_tag="RO454_000062"
     CDS             55057..56490
                     /gene="rpoN"
                     /locus_tag="RO454_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDY0931955.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56513..56800
                     /gene="hpf"
                     /locus_tag="RO454_000063"
     CDS             56513..56800
                     /gene="hpf"
                     /locus_tag="RO454_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDY0931956.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56918..57409
                     /gene="ptsN"
                     /locus_tag="RO454_000064"
     CDS             56918..57409
                     /gene="ptsN"
                     /locus_tag="RO454_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDY0931957.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57455..58309
                     /gene="rapZ"
                     /locus_tag="RO454_000065"
     CDS             57455..58309
                     /gene="rapZ"
                     /locus_tag="RO454_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDY0931958.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58306..58578
                     /gene="npr"
                     /locus_tag="RO454_000066"
     CDS             58306..58578
                     /gene="npr"
                     /locus_tag="RO454_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDY0931959.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58791..59423
                     /gene="yrbL"
                     /locus_tag="RO454_000067"
     CDS             58791..59423
                     /gene="yrbL"
                     /locus_tag="RO454_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDY0931960.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59420..60148)
                     /gene="mtgA"
                     /locus_tag="RO454_000068"
     CDS             complement(59420..60148)
                     /gene="mtgA"
                     /locus_tag="RO454_000068"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDY0931961.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(60145..60798)
                     /gene="elbB"
                     /locus_tag="RO454_000069"
     CDS             complement(60145..60798)
                     /gene="elbB"
                     /locus_tag="RO454_000069"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDY0931962.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(61028..63364)
                     /gene="arcB"
                     /locus_tag="RO454_000070"
     CDS             complement(61028..63364)
                     /gene="arcB"
                     /locus_tag="RO454_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDY0931963.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63460..64389)
                     /locus_tag="RO454_000071"
     CDS             complement(63460..64389)
                     /locus_tag="RO454_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDY0931964.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            65064..69524
                     /gene="gltB"
                     /locus_tag="RO454_000072"
     CDS             65064..69524
                     /gene="gltB"
                     /locus_tag="RO454_000072"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDY0931965.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69537..70955
                     /gene="gltD"
                     /locus_tag="RO454_000073"
     CDS             69537..70955
                     /gene="gltD"
                     /locus_tag="RO454_000073"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDY0931966.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(71023..71487)
                     /locus_tag="RO454_000074"
     CDS             complement(71023..71487)
                     /locus_tag="RO454_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDY0931967.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(71484..72359)
                     /gene="nanK"
                     /locus_tag="RO454_000075"
     CDS             complement(71484..72359)
                     /gene="nanK"
                     /locus_tag="RO454_000075"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDY0931968.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(72356..73045)
                     /locus_tag="RO454_000076"
     CDS             complement(72356..73045)
                     /locus_tag="RO454_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDY0931969.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(73093..74583)
                     /gene="nanT"
                     /locus_tag="RO454_000077"
     CDS             complement(73093..74583)
                     /gene="nanT"
                     /locus_tag="RO454_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDY0931970.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(74692..75585)
                     /gene="nanA"
                     /locus_tag="RO454_000078"
     CDS             complement(74692..75585)
                     /gene="nanA"
                     /locus_tag="RO454_000078"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDY0931971.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(75707..76498)
                     /gene="nanR"
                     /locus_tag="RO454_000079"
     CDS             complement(75707..76498)
                     /gene="nanR"
                     /locus_tag="RO454_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDY0931972.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(76606..77103)
                     /gene="sspB"
                     /locus_tag="RO454_000080"
     CDS             complement(76606..77103)
                     /gene="sspB"
                     /locus_tag="RO454_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDY0931973.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77109..77747)
                     /gene="sspA"
                     /locus_tag="RO454_000081"
     CDS             complement(77109..77747)
                     /gene="sspA"
                     /locus_tag="RO454_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDY0931974.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78142..78534)
                     /gene="rpsI"
                     /locus_tag="RO454_000082"
     CDS             complement(78142..78534)
                     /gene="rpsI"
                     /locus_tag="RO454_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDY0931975.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78550..78978)
                     /gene="rplM"
                     /locus_tag="RO454_000083"
     CDS             complement(78550..78978)
                     /gene="rplM"
                     /locus_tag="RO454_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDY0931976.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79198..80325)
                     /gene="zapE"
                     /locus_tag="RO454_000084"
     CDS             complement(79198..80325)
                     /gene="zapE"
                     /locus_tag="RO454_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDY0931977.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80519..80917
                     /locus_tag="RO454_000085"
     CDS             80519..80917
                     /locus_tag="RO454_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDY0931978.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81071..82438
                     /gene="degQ"
                     /locus_tag="RO454_000086"
     CDS             81071..82438
                     /gene="degQ"
                     /locus_tag="RO454_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDY0931979.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            82528..83595
                     /gene="degS"
                     /locus_tag="RO454_000087"
     CDS             82528..83595
                     /gene="degS"
                     /locus_tag="RO454_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDY0931980.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83658..84596)
                     /gene="mdh"
                     /locus_tag="RO454_000088"
     CDS             complement(83658..84596)
                     /gene="mdh"
                     /locus_tag="RO454_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDY0931981.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85031..85501
                     /gene="argR"
                     /locus_tag="RO454_000089"
     CDS             85031..85501
                     /gene="argR"
                     /locus_tag="RO454_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDY0931982.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
     gene            85865..86128
                     /gene="yhcN"
                     /locus_tag="RO454_000090"
     CDS             85865..86128
                     /gene="yhcN"
                     /locus_tag="RO454_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDY0931983.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86183..86455)
                     /locus_tag="RO454_000091"
     CDS             complement(86183..86455)
                     /locus_tag="RO454_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDY0931984.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86547..88514)
                     /gene="aaeB"
                     /locus_tag="RO454_000092"
     CDS             complement(86547..88514)
                     /gene="aaeB"
                     /locus_tag="RO454_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDY0931985.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPCMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(88520..89452)
                     /gene="aaeA"
                     /locus_tag="RO454_000093"
     CDS             complement(88520..89452)
                     /gene="aaeA"
                     /locus_tag="RO454_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDY0931986.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(89460..89663)
                     /locus_tag="RO454_000094"
     CDS             complement(89460..89663)
                     /locus_tag="RO454_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDY0931987.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            89846..90775
                     /gene="aaeR"
                     /locus_tag="RO454_000095"
     CDS             89846..90775
                     /gene="aaeR"
                     /locus_tag="RO454_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDY0931988.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(90897..92342)
                     /gene="tldD"
                     /locus_tag="RO454_000096"
     CDS             complement(90897..92342)
                     /gene="tldD"
                     /locus_tag="RO454_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDY0931989.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(92498..96298)
                     /gene="yhdP"
                     /locus_tag="RO454_000097"
     CDS             complement(92498..96298)
                     /gene="yhdP"
                     /locus_tag="RO454_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDY0931990.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(96366..97835)
                     /gene="rng"
                     /locus_tag="RO454_000098"
     CDS             complement(96366..97835)
                     /gene="rng"
                     /locus_tag="RO454_000098"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDY0931991.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(97825..98418)
                     /gene="yhdE"
                     /locus_tag="RO454_000099"
     CDS             complement(97825..98418)
                     /gene="yhdE"
                     /locus_tag="RO454_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDY0931992.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(98427..98915)
                     /gene="mreD"
                     /locus_tag="RO454_000100"
     CDS             complement(98427..98915)
                     /gene="mreD"
                     /locus_tag="RO454_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDY0931993.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(98915..100018)
                     /gene="mreC"
                     /locus_tag="RO454_000101"
     CDS             complement(98915..100018)
                     /gene="mreC"
                     /locus_tag="RO454_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDY0931994.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(100084..101127)
                     /gene="mreB"
                     /locus_tag="RO454_000102"
     CDS             complement(100084..101127)
                     /gene="mreB"
                     /locus_tag="RO454_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDY0931995.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(101432..103372)
                     /gene="csrD"
                     /locus_tag="RO454_000103"
     CDS             complement(101432..103372)
                     /gene="csrD"
                     /locus_tag="RO454_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDY0931996.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            103524..104498
                     /locus_tag="RO454_000104"
     CDS             103524..104498
                     /locus_tag="RO454_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDY0931997.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            105475..105945
                     /gene="accB"
                     /locus_tag="RO454_000105"
                     /gene_synonym="fabE"
     CDS             105475..105945
                     /gene="accB"
                     /locus_tag="RO454_000105"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDY0931998.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            105956..107305
                     /gene="accC"
                     /locus_tag="RO454_000106"
     CDS             105956..107305
                     /gene="accC"
                     /locus_tag="RO454_000106"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDY0931999.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(107397..108443)
                     /locus_tag="RO454_000107"
     CDS             complement(107397..108443)
                     /locus_tag="RO454_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDY0932000.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(108440..109402)
                     /locus_tag="RO454_000108"
     CDS             complement(108440..109402)
                     /locus_tag="RO454_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0932001.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(109424..110413)
                     /locus_tag="RO454_000109"
     CDS             complement(109424..110413)
                     /locus_tag="RO454_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932002.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(110414..111913)
                     /locus_tag="RO454_000110"
     CDS             complement(110414..111913)
                     /locus_tag="RO454_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDY0932003.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(111974..112864)
                     /locus_tag="RO454_000111"
     CDS             complement(111974..112864)
                     /locus_tag="RO454_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDY0932004.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(112900..113754)
                     /locus_tag="RO454_000112"
     CDS             complement(112900..113754)
                     /locus_tag="RO454_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HDY0932005.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT        28106 a        30674 c        28013 g        27073 t
ORIGIN      
        1 atattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa atattctctt
       61 aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga catccaccat
      121 cggtgcgaat ctgttggtag tcgtggatga tgttgtcgct aacgatgata ttcaacagaa
      181 attaatgggt attaccgcag aaacctacgg ctttggcatt cgtttcttta ctatcgaaaa
      241 aaccattaac gtcatcggca aagctgcacc acatcagaag atcttcctga tttgccgtac
      301 gccacaaacg gtacgtaaat tggtagaagg tggtattgac ctgaaagatg tcaacgtcgg
      361 caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg tcgatgacca
      421 ggatctcacg gacttacgtt atattaaaca acgtggcgtg aatgttttca ttcaggacgt
      481 tcctggcgat caaaaagaac aaatccctga ctaaatctga aatcgcctta atattggttt
      541 gaggtaataa aaatgcatga aataacccta ctccagggat tatccctggc ggcgttagtt
      601 ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc gataatcgtt
      661 tgtaccctta caggtgctat tctcggtgat attcagactg gcttaattac cggaggcctg
      721 acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc caaccctatt
      781 atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc caaaacggca
      841 attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt cttctattcc
      901 gctttctcat tatttatgac caaagccgat aaatgcgcga aagaggcgga tacggcagcg
      961 ttttcccgac ttaactggac aacgatgctc atcgtcgctt cagcgtatgc ggtgattgct
     1021 ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc gatgcccgcc
     1081 tggctgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg ttttggcttg
     1141 ctgctgcgcg tgatgttcaa agcgcaatat atcccttacc tgatcgccgg tttcctgttt
     1201 gtttgctaca tccaggtcag caacctgttg ccggttgccg tgctgggcgc aggctttgcg
     1261 gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt
     1321 aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag atatcacccg
     1381 tctgggcttt cgttcgtcac tgctacaagc aagctttaac tacgaaagga tgcaggcggg
     1441 cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataagaacg acaaaccggg
     1501 cctaagcgcg gcgatgaaag ataacctcga atttattaat acccacccta atctggtcgg
     1561 atttctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg acaccattaa
     1621 aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga ttttctggtt
     1681 tactttgttg ccgattatgg cggggatttg ctcatcgttt gccagtcagg ggaacctgtt
     1741 gggaccgatt ttgtttttcg ccgtttacct gcttatcttt ttcctgcgcg tcggctggac
     1801 ccacgtcggt tattcagtcg gcgtgaaggc tattgataaa gtgcgagaga actcgcagat
     1861 gattgcccgt tcggcaacca tcctcgggat cacagtaatc ggcgggctga tcgcttcgta
     1921 tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg tcgcgctgca
     1981 gcaggatttc ttcgataaag tcttcccgaa tattttaccg atggcctaca ccctgctgat
     2041 gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg tgacgtttgt
     2101 tctttctatt gtttgttctg cattcggcat tttgtaaatg gaacgaggca ccgcgtctgg
     2161 cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcaacaag ttgaaaacta
     2221 tacggcgtta agtgaacgtg ccagcgaata tttattggcc gtgatccgta gcaaaccgga
     2281 tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt atttggtaga
     2341 aaaaatccac cagcagcagg ttgatgtcag ccagctcacc ttcgtgaagc tcgacgaatg
     2401 ggtggatctg ccattaacga tgcctggtac ctgcgaaact ttccttcaac agcatatcgt
     2461 gcagccgctg gggctacgtg aagaccagct catcagcttt cactccgaag agataaatga
     2521 gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg acttatgcgt
     2581 tctcggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa gcctgcaacc
     2641 ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt taaaaaccgc
     2701 tgaccgtccc gtgactcgtg ggatcacctt aggcctgaag gatattctca atgcccgcga
     2761 agttttgtta ctgataactg gcgaaggaaa acaggatgcg acagaacgtt ttctcacggc
     2821 taaagtctcc accgctatcc cggcttcatt tttatggctg cataaccatt tcacatgcgt
     2881 gattgatgaa atgtgtcgtc gttaactcgc ctggtacagg cgttgcagcg ctattcctgc
     2941 tgcgacgcct gtaatcactc tccctgctgc tccagcgcat acttatacag cgcatttttc
     3001 ttcacgccgt gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt
     3061 aacagcgcca gcgtgcgcag ggcatcagca ggcaggtcat cttcctgcgc tttatggcct
     3121 tcgacaatca gcaccatttc gcctttgcga cggttttcat cttcttttac ccacgccagc
     3181 agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt tggttagctc acgcgccaga
     3241 accacgtaac gggattcgcc taataccgca acaatatctt ccaggctatc taacagacgg
     3301 tgggtagatt cataaaaaat cagcgtgcgc ggctccgctt caatagcttt tagcgcatca
     3361 cggcggcctt ttgatttggc aggtaaaaag ccttcataac aaaaacgatc ggatggtaaa
     3421 cccgcggcgc ttaacgcagt gatagcagca cacggccctg gtagcggcac cacgcgaatc
     3481 cccgcttcac gacaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg
     3541 gcatcggaaa ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct
     3601 ttttgttgtt cgttatgatc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc
     3661 agcaataaac cggtgtgacg cgtatcctcg gcggcaatca gatcaacggc ctgtaatacc
     3721 tctaatgcac gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc
     3781 tgcccctgag aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt
     3841 taatattgag cattgcgtaa aaaaaatatc actggataca ttatggtacc ctcaacattt
     3901 tctcgtttga aagccgcgcg ttgtctgcct gttgttttgg cagccctgat tttcgccggt
     3961 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     4021 tctgcttttt acctgcaaca gatgcagcaa agttctgatg ataccaggat caactggcaa
     4081 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttggtt
     4141 aaccaactac cgcaagaact gaatgataat cagcgtcgcg agaaaacact gctggcggca
     4201 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaatcaca
     4261 cctgccgatt tagaacaaaa ccagcaagcc cgttactggc aggcaaaaat cgatgccagc
     4321 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggt
     4381 gcgaaagaaa agcagcagaa tattgacgcc acatggcagg cgctctcctc catgactcag
     4441 gagcaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     4501 ttgcagcgcg tctggtttga taaccgtaac gatcctgaca tgatgaaagc tgggatcgcc
     4561 gactggcaga aacgttatcc gaacaacccg ggcgcgaaaa tgctgccaac gcagttggtt
     4621 aacgtaaaag cgtttaaacc agcatcgacc aacaaaatcg ccctgctgtt gccgctgaat
     4681 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     4741 ggcactcagc cagtggcggc ccaggtagct gccgcacctg ccgcagacgt agcagaacaa
     4801 cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcttcggt tagcgatctg
     4861 accggtgaac agcctgcggc ccagccggta cctgtaagcg ccccggcgac aagcaccgca
     4921 gcggtaaacg cacccgcaaa tccatccgca gagctgaaaa tctacgatac ctcatcacaa
     4981 ccacttagcc agatcttaag ccaggttcag caggatggtg cgagtattgt ggtcggtccg
     5041 ttgctgaaaa ataacgttga agagttgctg aagagcaaca ccccgctgaa cgtgctggca
     5101 ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcgcca
     5161 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     5221 gtgctgatcc cgcgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaagagtgg
     5281 cagaaactgg gtggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     5341 gcgggtgtta acggcggttc tggtattgct ttaacgggta gcccgattac tcccagagcg
     5401 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     5461 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     5521 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     5581 tacgccagtt cccgcagtgc gcaagggacc gctggcccgg atttccgcct ggagatggaa
     5641 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctgccgtt aatgcagcag
     5701 gcgctcagcg cggtgaacaa cgattattcg ctggctcgca tgtatgcgat gggtgtcgat
     5761 gcctggtcgt tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac
     5821 ggtaataccg gaagcctgac ggcaagcccg gattgcgtga ttaacaggaa gttatcatgg
     5881 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     5941 cgtgggaagc acaagcgcgt cgctggctgg aaggcaaagg actacggttt atcgccgcta
     6001 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     6061 ttatcgaggt gcgttaccgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     6121 gcagcaaaca acacaaatta ttacagactg cccgtttgtg gctcgcgcgt cataatggga
     6181 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     6241 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     6301 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     6361 ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     6421 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     6481 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     6541 gttgtcttaa cggcaattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     6601 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc
     6661 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgaccattgt ggcattaacc
     6721 ggctatgacg gcggtgaact tgcaggtttg ttagggccac aggatgtgga gatccgcatt
     6781 ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     6841 gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata catgaaggca
     6901 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     6961 gtagtgggta ctgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     7021 caggtagacg atggtacctt ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacaa
     7081 attaagaaag aagcgcgcat taatgtaacg gcctatcagg gcaaagtgct gctggttggg
     7141 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     7201 gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcgtct
     7261 aacgatacgt ggatcaccac caaagtacgt tcgcagctgt taaccagcga cctggtgaaa
     7321 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     7381 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg taagcggcgt gaagcgggta
     7441 actacggcgt ttacgtttat taaatagcag cccttgtaat gcctgatgcg acgcttgcag
     7501 cgtcttatca ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc
     7561 cgacatgttt tccctcaaat caaactaaca gcgccagccc gccgacaatc acaccagaca
     7621 ctgccaccat cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa
     7681 tcagtgacgg caaactcacc gccgggagcg tcatcaacaa tgccagcgcc ggagcagttc
     7741 ccatacctgc cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa
     7801 acaagcatcc tgccaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat
     7861 cggcatgggg gaataaccag acgcgagcgg cacccaacac cagtactgca aggatgtaaa
     7921 ccgggatcgt actccagaaa agcgtccata gcgccctgcc ccagcggcta aaaaatcctc
     7981 cctgtgcttc cggtatatca atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc
     8041 atttttgcac cagcgtcgca atcagcaaca ccatcaccag ccccgccacc agacgaatcg
     8101 ccgcaaaacc ccagccgagg acgaacccca taaacaccag cgtcgccggg tttaacaacg
     8161 gattccccat ccagaatgcc agcgcaccgc ccatcgacac ctgttggcga cgcattcccg
     8221 ccgcgaccgg agccgcacag caggtacaca tcatacccgg cagcgaaaac agcgttccca
     8281 gcagcgtgcc gcgaaagcgc gattgcccaa gcgtacgcaa caaccagtca cgcgggatca
     8341 gtacctgaat caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga
     8401 aatagatcat cgcgtaatcc aacgccgcct gccacggatt agcatccgcc tgcgcaagga
     8461 tagatttacc gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc
     8521 atttcacata ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact
     8581 gaatgggcgt tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt
     8641 tatgataatt gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag
     8701 ctcttttgcg taattctgaa gagcttaaaa tcgtcacacc ttcatgctca ggccgcatcg
     8761 cctctgccag catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggta
     8821 acaaacggaa cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcaccca
     8881 gtaacatcga cgggcgagca atggtcaatt tcggccagtt ctgggcgatt aatgcttctt
     8941 ccatctcccc tttgacgcgg ttatagaaaa acggcgagtg ggcattagca cccatagcac
     9001 tgaccaccag catatgctgc gcaccgagcc gccgcccggt taatgcggta tccaccacca
     9061 acgtgtaatc ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga
     9121 gacaacaaaa cacaatgtcg atgggatcgg tgacctgcgc cagcgcgtca gtcagttgcg
     9181 gatcatgggg attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcagcaa
     9241 tgacgttaac tttcggttca ttaatcaaca tccgcagcag gtgaccgccc accagccccg
     9301 ttgcgcctgt aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg
     9361 ctccccagct catgcaccag gcttaataga ccatgaggta attatccccg attgtggaaa
     9421 attcgcctca tccaatgcaa caacgcggag gaagcatgag taagaaaatc gccgctttaa
     9481 tcactgatga atttgaggat tcagaattca cttcacccgc agacgagttc cgtaaagccg
     9541 gacacgaagt gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag
     9601 aagccagcgt ggtcatcgat aaatccatcg atgaagtgac gcccgcagag ttcgatgccc
     9661 tgctgctacc gggcggtcat tcaccagatt atctgcgtgg cgacaaccgt tttgtcacct
     9721 tcactcgcga ttttgtgaac agcggcaaac ctgttttcgc catctgtcac ggtccccagt
     9781 tgctgatcag cgccgatgtg atccgtggtc gtaagctgac cgccgttaaa ccgatcgtga
     9841 ttgatgttaa aaacgcgggc gcggaatttt acgatcagga agtggtggtc gataaagatc
     9901 agctggttac cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc
     9961 tgctcggtgc ctgagttacg tagccaaatc atttttttac caaagcccag cgtgttgtcg
    10021 gtgaatttga tttcatcgag ccggatttcc cataccgggg ccgacagcat tctggcaacc
    10081 ggaaagcgac gattgtacgc cttgcgtgcg aggtcgcttt cctcgccttc cagtctgcgg
    10141 atctcacctt taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc
    10201 gttccggcaa ttgccgcctg cggcccgctc atctgcgcat gacgcgtttt ttcttccgtc
    10261 aaaatgtaga aggcgacttt ctgcgcatca aaaagataaa aggcattggc gcaccacagt
    10321 tccccttcct gctgcacaca ccaggtgaca acatgttgtt ttgccagcca acggctgatg
    10381 gcggtgagtg tttccattgc tgttctcttt tatactgtgg gcctgacctt aacatgcgac
    10441 gcagacgatg acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac
    10501 cgggatcacc acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa
    10561 agcactgcga gggaaaggag aactaacgct ggcgttttcc gcgccagtgg gcgaccgttc
    10621 gctggcacta cgagcggaat atcgcgttaa acaactgact aaacggcaaa aagagcgtct
    10681 ggtaacggaa ggcgcagggt ttgcagaact gttgagcagc ctgcaaaccc cggagattaa
    10741 aagcgattga aatgctcgtg atactcgacc agaccagtta cgccgttcag cgcgtcatcc
    10801 gctaaacgat gtacctggaa ggcgctttca gtgcccggcc agcggcaatg cagatcgtga
    10861 tgcgccgcca gttcaaagcc gaaacggctg tacagcgccg gatcgcccag cgtcaccact
    10921 gcggcatagc cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc
    10981 ccctgtccac ggtatttttc atcaaccgcc agcggtgcca tgccgaccca ttgcaggtct
    11041 tcgccctgca catcaaccgg actaaaagcc acatagccaa tgacctgacc ttcgtcatct
    11101 gtcgccacca gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct
    11161 tccgcatcac tttcgaatga acgacgcagc agggcatcaa tacccggcgc atcaatggga
    11221 atttctactc gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt
    11281 tcattcccgc ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca
    11341 tttgctccag ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc
    11401 cttcaatcac cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa
    11461 tcatcagcag atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctaa
    11521 cgaccagttt gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa
    11581 tacttaacca gcgaccttca agaaactcca gctcgccatc atccagcgcc tgacggaatt
    11641 gccagcttaa gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg
    11701 gtacactcaa cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga
    11761 gttttactcc ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg
    11821 tataaatcaa caattccata tgaaattgct gctaccacca atacaacttt aactgcctta
    11881 aatcaaaaat tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt
    11941 tgatagcgca accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg
    12001 cgcttaaggc ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata
    12061 ccaatgcccg tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga
    12121 gttttgtcca tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg
    12181 acggttacgc ccgttggcag cacgccgtgg atatggcggc gcaactgggt gccgacgcgc
    12241 tgatcctcgc cgacctcgcc atgctggagt acgccgccga acgttatccg catattgagc
    12301 gccacgtatc ggtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc
    12361 attttgacgt tgcccgcgtg gtactgccgc gcgtgttgtc gattcatcag gtgaaacagc
    12421 tggcacgggt cacacctgta ccactggaag tgtttgcttt cggcagcctg tgcattatgt
    12481 cggaaggccg ttgctatctg tcgtcgtatc tgacgggtga atcgcccaac accgtggggg
    12541 catgttctcc ggcccgtttc gtgcgctggc agcaaacgcc gcaagggctg gaatcccgcc
    12601 tgaacgaagt gctgatcgac cgttatcagg acggcgaaaa cgcaggttat ccgacgctgt
    12661 gtaaagggcg ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc
    12721 tcaataccct ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg
    12781 aaggccgcca gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta
    12841 tcgaccgttg taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc
    12901 tcgggtcgat gtccgaaggc acgcaaacta cccttggcgc gtatcaccgt aaatggcagt
    12961 gagaaaagca atgaaatatt ccttagggcc ggtgctatgg tactggccaa aagagacgct
    13021 ggaagacttt tatcagcagg ccgccgccag cagcgcagac gtgatttatc ttggtgaagc
    13081 ggtatgcagc aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcact
    13141 cgccggaagt ggtaagcaga ttgtgctctc cacgctggcg ctggtgcagg catcctctga
    13201 actgggcgaa ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct
    13261 cggcgtggtg aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa
    13321 ctgctacaac gcggtgacgc tgaaaatatt gcttaaacag ggcatgatgc gctggtgtat
    13381 gccggtggag ctttcccgcg actggctggt gaatctgctt aatcagtgcg atgagttggg
    13441 cattcgcaac cagtttgaag tggaagtcct gagctacggt catctaccgc tggcctactc
    13501 cgcacgctgc tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg
    13561 cattaagtat ccgaacgggc gcaacgtact gtcgcaggaa aaccaacaag tgtttgtact
    13621 caacggcatt cagaccatga gcggctacgt ttacaacctc ggtaacgagc tggcatccat
    13681 gcaggggctg gtcgatgtgg tccgcctgtc accgcagggt actgacactt tcgcaatgct
    13741 cgatgccttc cgcgctaatg aaaatggcgc tgcgccacta ccgttgacgg caaacagcga
    13801 ctgtaacggc tactggcggc ggctggccgg gctggagctg caagcgtaag tatatagctc
    13861 actttgttaa caactttaac tactctttaa tgcagtatta aagactaatc ggtaacaaag
    13921 tgagctgtta tgactgataa aaccattgcg ttttcgctac tcgatctggc ccccatcccc
    13981 gaaggttctt cagcgcggga agcgttctcc cactctctcg acctcgcccg tctggctgaa
    14041 aagcgcggct atcatcgcta ctggctggca gaacaccaca atatgaccgg cattgccagt
    14101 gctgcaacgt cggtgttaat tggctatctg gcggcgaata ccaccacgct gcatctgggg
    14161 tctggcggcg tgatgttgcc taaccactca ccgttggtca ttgccgaaca gttcggcacg
    14221 cttaatacgc tctatccggg gcgaatcgat ttggggctgg gtcgcgcgcc gggtagcgac
    14281 caacggacaa tgatggcgct acgtcgtcat atgagcggcg atattgataa tttccctcgc
    14341 gatgtcgcgg aactggtgga ctggtttgat gcccgcgatc ccaatccgca tgtgcgccct
    14401 gtaccgggct atggcgagaa aatcccggtg tggttgctag gctccagcct ttacagtgca
    14461 caactggcgg cgcagcttgg tctgccgttc gcgtttgcct cacacttcgc gccggatatg
    14521 ctgttccagg cgctgcatct ttatcgcagc aacttcaaac cgtcggcacg gctggagaaa
    14581 ccgtatgcga tggtgtgcat caatattatt gccgccgaca gtaaccgcga cgctgagttt
    14641 ctgtttacct caatgcagca agcctttgtg aagctgcgtc gcggcgaaac cgggcaactg
    14701 ccgccaccga ttcaaaatat ggatcagttc tggtcaccgt ccgaacagta tggcgtgcag
    14761 caggcgctaa gtatgtcgct ggtgggcgat aaagcgaaag tgcgtcatgg cttgcagtcg
    14821 atcctgcgcg aaaccgacgc cgatgagatt atggttaacg ggcagatttt cgaccaccag
    14881 gcacgcctgc attcgtttga gctggcgatg gatgtgaagg aagagttgtt ggggtagtgt
    14941 gttttaacgt aggaagcctt atccgagatg gcaacgctgt cctacgtagg cctgataagc
    15001 gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcacct ctgattactg
    15061 atacaccggc agcacattaa agctcgataa gatatgcacc agtgcgttgc cgacgccaaa
    15121 caccagaatc agcacaatca tcggcttgcc gccccataca cggaatttcg ggctaccaaa
    15181 gcgtttacgc gatgcgcggg ccagcagcgc cggaacaatt gccgcccaga tggtagccgc
    15241 taagccagca taaccaatgg cgtacaggaa tccgttagga aacagcaggc cacccacaac
    15301 cggcggggca aaggtcagca acgctgtttt caaacggccc acagccgagt cgtcgaaacc
    15361 aaacagatct gccagatagt caaacaggcc cagcgttacg ccaaggaacg aactcgctac
    15421 cgcaaagttt gagaacacaa ccagcagaag gtccaggcta cggctgttca gtacgccgct
    15481 taacgcctgt accagcacat caatattacc gcccttctcg gcgatgccga taaactccgg
    15541 acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca gcgccatcag
    15601 cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat acttcatcag
    15661 gctcggtacg ttaccgtgat aaccaaacga tgccagacag aacggcagcg tcatcaacag
    15721 atacggtgca taagacgcat tgctttcggc gacgttgaac aatgtcgcag gctgcacatg
    15781 ccccagcagg ctaccgaagg tgaggaagaa ggtaatgact ttcgccccca gcacaatcgc
    15841 tgtcatgcga ctgacggctt tggtgctcaa ccacaccaca aacgccacca acagtgcaaa
    15901 accaaaaccc gctgcccgtg ccgggacgtt tagcgacatc tctgcgaagg tgtgatgcag
    15961 aatcgaacca ctggcagaaa tataggcata ggtcaggatg tagagcacaa aggcaatgga
    16021 aatgccgttg accacgttcc agcctttgcc gagcaggtct ttggtgatgg tgtcaaagct
    16081 cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat gcagcataca
    16141 gaaccaggtg aaaatcagcg ccgccattga ccagaagaac cacgccccgg acatgaccac
    16201 tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca cgccgccaag
    16261 cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc tccagtgaaa
    16321 aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acatgagtac aaaaaacaga
    16381 aaaaaagccc cgatgggtaa aatcggggct gtatatatta ttttacagat tgtgttcgct
    16441 gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt catcacgacg
    16501 cggtgcgcgg ccttcacgac gttcgccgct aaaacgacgc ccatcaccac ggccaccttc
    16561 acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga aaccacgacc
    16621 accgccacga cgctcaccgc cagtatgcgg ctgtgcatcg cccagtaact gcatgttcat
    16681 tggcttgttg agaatgcgag tacgcgtaaa gtgttgcagc acttcacccg gcataccttt
    16741 cggcagttcg atggtggagt gagaagcaaa cagcttgatg ttaccgatgt aacggctgct
    16801 gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac catcatcgcg
    16861 gcccacttca atacggtaca actgcatatc gccaacatca cgacgttcgc gacgcggacg
    16921 atcttcacgg tcgccacgcg ggccacggtc gttacgatcg cgcggaccac ggtcatcacg
    16981 gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca gagtacgttc
    17041 accctgtgcc attttcagta gtgcggctgc cagagtttcg agatccagct cttcaccttc
    17101 ggcagtcggc tgaattttgc tcagcagtgc acggtattga tccagatcgc tgctttccag
    17161 ctgctgctgt actttagcgg caaatttttc cagacgacgt ttgcccagca gttctgcgtt
    17221 cggcagttct acttccggaa tggtcagctt catggtgcgt tcgatgttgc gcagcagacg
    17281 acgctcacgg ttctcaacga acagcagtgc acgaccagca cgacctgcac gaccggtacg
    17341 accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa ccaccaggct
    17401 gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga tgtccagacg
    17461 accatctttc aggcgttcca gtgtctgctc acgcagcgcc tggttcatgt caccgttcag
    17521 cgcggcgctg ttgtagccgt tacgctcaag agcttctgcc acttccagag tcgcgttttt
    17581 ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac gtaccagtgc
    17641 ttcgttttta cgcatacccc agacagtcca gtagctctgg ctgatgtcag gacgggtagt
    17701 cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg taatgcgacg
    17761 aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg ggatctgcgc
    17821 cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag cttcatccag
    17881 aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt ccagcagacg
    17941 gcccggtgta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt gcacgtcata
    18001 acgttggccg ccgtacaggg caaccacgtt tacgccgcgc atgtgtttag agaaatccgt
    18061 cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca gaatctgtgg
    18121 tgctttcagc tcaggatcaa gattctgcag cagaggtaaa gagaatgctg cagtttttcc
    18181 gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg gaatacactc
    18241 tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa ggataggagc
    18301 cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg tgtgcctcaa
    18361 aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt ttcattgaaa
    18421 agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac ccgttaaggt
    18481 gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg gtccgattct
    18541 gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta tcggtgctca
    18601 acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc gctgtccaaa
    18661 tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag atgctcagcg
    18721 agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg ttcgctaatg
    18781 ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga tttttcgaag
    18841 tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc gagataaagc
    18901 cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag atcatcttgc
    18961 gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg cgcgtagttg
    19021 taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc aaaattgcct
    19081 gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg tcggattgcc
    19141 agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc atacaacact
    19201 ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact ggcaaggatt
    19261 tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg taccgcgagg
    19321 acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc tgtcgcaacg
    19381 aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt gaatgaacgt
    19441 cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc cgcagcggag
    19501 ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc tgcagcaggt
    19561 tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg atcaacttcc
    19621 agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt ctcaacgcgt
    19681 ttatcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat ggcaacaaat
    19741 gcgccaaagt caacgattcg ggtcacttta ccattgtaga cgcggcctac ttcgatttct
    19801 gcagtgatct cttcgatacg acgaatagcg tttttcgcct tctcgccgtc ggtcgctgcg
    19861 atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc ggtcagagca
    19921 cggattacag aaccgccttt accgataacg tctttgatct tgtctggatt gatcttgatg
    19981 gtatggatac gcggagcgaa ctcagagata tcaccgcgcg gcgcattgat cgcctgttcc
    20041 attacgccca ggatatgcag acgcgcacct ttagcctggt tcagtgcaac ctgcatgatc
    20101 tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc gtcacgagaa
    20161 cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat gtcagacagt
    20221 acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac ggcagctttg
    20281 atcggcacac cggcgtccat cagcgccaga gacgcgccgc acacggaagc catagaagag
    20341 gaaccgttgg attcggtgat ttcagacaca acacgtacgg tgtacgggaa tttgtccata
    20401 tccggcatta ctgccagcac gccgcgcttc gccagacgac cgtgaccaat ttcacgacgc
    20461 ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt gtagtggaac
    20521 aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc acgagcagta
    20581 cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc agaaccgtga
    20641 gtacgcggca gcacaccagt acgcacatcc agaccacgga tcatatcttt ttcacgaccg
    20701 tcgatacgcg gttcgccggc cagtacgcgg ctacgaacaa catttttctc gatagcgtgc
    20761 agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag tgtcgcgatg
    20821 gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt gatgcggtaa
    20881 gcatcgctca gacgagcttc agccagcgca gcaacgcgcg cgttcagcgc ttcgtttacc
    20941 ggctccggct gccaatccca acgcggttta ccggcttctt tcaccagttc attgatgttc
    21001 tgaataacaa cctgctgttg ttcatgaccg aacaccactg cgcccagcat ttggtcttcg
    21061 ctcagcagtt cagcttcaga ttcaaccatc agcacggcag cttctgtacc ggcaacaacc
    21121 aggtccagtt tgctctcttt cagttcgtcc tgagtcgggt tcagtacgta ctggtcattg
    21181 atgtaaccta cacgggcagc accaatcggg ccattgaacg gaataccaga cagagacagt
    21241 gctgcggaag caccaatcat cgcgacgata tccgggttaa cctgcgggtt aacagaaacc
    21301 acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg gcgaatcggg
    21361 cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc acgacggaag
    21421 aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac ggtcagcggg
    21481 aagaagtcct gacctggttt ggcttttttc tgaccaacaa cggtaacaaa taccgcggtg
    21541 tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc ggtttccaga
    21601 gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa tgtaatatcc
    21661 tttctctttc ttagacagta ccttacggta ctggtgttaa tacccgatct tctgcgcatc
    21721 ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc attagccgcg
    21781 cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca gtgaattgct
    21841 gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg caagaattag
    21901 cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt acgtttcagg
    21961 tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg gctgtggtga
    22021 tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt cagcagtgct
    22081 acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga aacgatttta
    22141 gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa tgaaaggacg
    22201 ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca atttacgcga
    22261 cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt aacattacgc
    22321 cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa tttcgcccat
    22381 accgataaac ttgccgttct caccctctgt aacgcgaacc agtccttcca gcggcgcacc
    22441 ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg gaagattcac
    22501 cactgggtag tccgaagctg gactgtccat tggcatcagt aatgggtcaa gtaactccgc
    22561 agccggaata tcctgctgtt cggcttgctc aaccagttca cgcagatgtt ccagggtcac
    22621 catccgttca accggatatt tacttaccgc cagacgacgc aagtaaataa catgcgcgcc
    22681 acaaccgagt ttttcgccga gatcgtcaat gatagtgcgg atataagtgc cttttgagca
    22741 gtgaatttcc agctccagct cattgccttc atggcgaata aacagcaatt cataaacggt
    22801 aatcgggcgc gcttcacgtg gaacttcaat gccctgacgc gcgtattcgt acagtttttt
    22861 gccctgatat ttcagtgctg aatacatcga aggaatctgt tcgatatcgc cacggaaggt
    22921 atccagcgcc gccgccagtt gctctgcact aaaggttacc ggacgttctt caacgatttg
    22981 tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc gataacgttt
    23041 gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa ttggcaacat
    23101 gccggtcgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt tataaatccg
    23161 tttcactttt tgcagcgcat cgttgctgga catcccctgc ggtttatcca gcaacaaaac
    23221 gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt gctgtcgtcc
    23281 gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt tgacatgcgc
    23341 atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat acgcaggcgc
    23401 atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cctgcaacgc tttgatgccc
    23461 gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt ggcatacgcc
    23521 aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccagacg aggatcttta
    23581 atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac gcgctgcggg
    23641 cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt tagccccttt
    23701 ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg cgccagcgtc
    23761 gcatcaggca atccatacag gatgcggcat aaacgcctta tcccgcatgg aaccctaaaa
    23821 accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca catcgccagt
    23881 gcggacatcg ttgtagttct taacgccgat accacattcc ataccgttac ggacttcgtt
    23941 aacgtcatct ttgaagcgtc gcagggactc cagctcgcct tcgtagataa ccacgttgtc
    24001 gcgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca tacaacctgc
    24061 gatagcacca aatttcggtg atttgaacac gtcacgaact tccgccagac cgataatctg
    24121 ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt cgatcaggtt
    24181 atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt tacgtgcaga
    24241 ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag ccagggtagc
    24301 gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa cttcgtcagt
    24361 agacagtttc agcaaggagt cagaaattgc ttccacagaa ccctgtacgt ctgccttcag
    24421 gacgatattc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt tctcgagttt
    24481 agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat aaagtgcaac
    24541 ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag ccggtacgcc
    24601 ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt cctgacccag
    24661 ttcgttacgc attgcacgaa cacgaccgta ttcgaagcca cacagaacga tatcgccctt
    24721 gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt tatcgaggaa
    24781 ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct ccagaacttc
    24841 cgcctgcagc aggatagcat ccagcagttc atcaataccg gtacccgctt ttgcagatac
    24901 gtgtacgaac tggctttcgc cgccccactc ttctggcagg atgccgtact gggagagttc
    24961 gttcttaacg cgatccggat cggcttctgg tttatcgatt ttgttcacag caaccaccac
    25021 cggaacaccc gccgctttcg cgtgctggat agcttcgatg gtctgcggca ttacaccgtc
    25081 gtctgcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg cacgcattga
    25141 agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt cagtttcaac
    25201 gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcca ctttcgttga
    25261 acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga tggtcacaac
    25321 cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg cctcttccag
    25381 ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca gctgtgcggt
    25441 ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca tcgctttgat
    25501 gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga tggtttcgcc
    25561 gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct gcagcgaaga
    25621 acctttacgt tttccgccct taccgccacg tactgctgca cgagcttctt cacgatcagc
    25681 ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt tacgaccacg
    25741 gccacggccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag catgttgaga
    25801 agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag tccatttgtt
    25861 ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt cgagtttacg
    25921 acgcgcttct tcttcggctt tacgcttgag ctctgcagct tcctgctcac ggcgggcttt
    25981 ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt ctttttccgc
    26041 agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg cctcacgttc
    26101 agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg cttgctcttc
    26161 cgcttcacgc tgcgcttgct cttccgctgc aaggcgctca gcctcttgcg gatcgcgttt
    26221 cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc
    26281 aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt
    26341 tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc
    26401 agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg
    26461 ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta
    26521 ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcttt
    26581 ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc
    26641 cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag
    26701 gttcagcaga tcatcagccg gtttgttatc accgaggctt tcttcctggg cctgtgcaat
    26761 ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc
    26821 ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaagcc
    26881 ttcttctacc agaacagtcg cgaagtcttc atcgatgtcg agatatttgg tgaaggtgtc
    26941 gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcatcacgtt
    27001 gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc
    27061 ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac
    27121 gatagaagca acgtctgccg gtgccattgc gttaatcacg aactgcgccg ggttatcatc
    27181 ccacaggacg atatcgatac gctctccgcc cagttcagta gaaaccgcct gaacacgcgc
    27241 gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc
    27301 aattttcgca cgagatcccg ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc
    27361 gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg agcgggtgac
    27421 gaacagctgc gcgccacgcg cttccgggcg aacggaatag agcacgccac gaacgcggtc
    27481 gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt
    27541 gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat
    27601 ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt
    27661 ctgcacgata acctgttttg ccgtctgggt agtgatacga tcaaaggtaa cagactcaat
    27721 ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg cggcttcaag
    27781 ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc
    27841 aaaatcaccg cttttgcgat cgatctgtac gcgaacgtcg atttcttgtt catatttttt
    27901 ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc
    27961 cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc
    28021 acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat tactcagcgc
    28081 gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat
    28141 aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc
    28201 tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga
    28261 gacttccagg ttataagcaa cggtgatggg gtcttcaaca tccagcacag cacttacctg
    28321 gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg
    28381 cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac ccagggcctc
    28441 aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa
    28501 gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat
    28561 aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca
    28621 gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg
    28681 actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc
    28741 ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt
    28801 gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgcg
    28861 ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcac gtgaatgaat
    28921 atccagttca ctttcatttg ttgaatactt ttaccttctc ctgctttccc ttaagcgcat
    28981 tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat taaatgaaaa
    29041 ctcatttatt ttgcataaaa attcagtgag agcagaaatc caggctcatc atcagttaat
    29101 taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt
    29161 ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat gcgacaaaag
    29221 ggagctgttc cttatgcata tactgcaaac ctgggccagc cagacgaaga ggattatgat
    29281 gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat cgactgccgc
    29341 aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca taacaccact
    29401 ggtggactga cctatttcaa cacgacgccg ctgggccgcg ccgtgaccgg caccatgctg
    29461 gttgctgcta tgaaagaaga tggcgtgaat atctggggtg acggcagcac ctataaagga
    29521 aacgatatcg aacgtttcta ccgttacggt ctgctgacca atgctgaact gcagatttac
    29581 aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga gatgtctgaa
    29641 tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagctta ctccacggac
    29701 tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc
    29761 aaaatcgtca acccaattat gggcgtgaag ttttgggatg agagcgtgaa aatcccggca
    29821 gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg taaaaccttt
    29881 agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca cggcctgggc
    29941 atgagcgacc agattgaaaa ccgtatcatc gaagcaaaaa gccgcggcat ttatgaagcc
    30001 cctgggatgg cactgctgca cattgcgtat gaacgtttgt tgaccggtat tcacaacgaa
    30061 gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct gtaccaggga
    30121 cgttggtttg actcccaggc gctgatgctg cgggactctc tgcaacgctg ggttgccagc
    30181 cagatcactg gtgaagttac tctggagctg cgccgcggga acgattattc aatcctgaat
    30241 accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa aggcgactcg
    30301 gtgttctcgc cagatgatcg tatcggtcaa ctgaccatgc gtaacctgga tatcaccgat
    30361 acccgcgaga aacttttcgg ttatgccaaa actggcctgc tctcctcctc tgccgcttca
    30421 ggggtgccgc aggtggagaa tctggaaaat aaaggccagt aattcgacat caaccctgcc
    30481 cttcaggggt ggggttacct tgattatcct ctgtaaactc ctttctgagc atcacataat
    30541 cgaaaacatc atggtttgca tccacaaccc gcgagtctcc tttatagtgt gcaatcactt
    30601 cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta ataatgtaat
    30661 tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca tccccaagaa
    30721 cggggctata ccagataaac atagggacat gatatgcctg ctggctagcc tccctgcctc
    30781 catgaaaata gacgttcttc ttcgtagggt cgcgttccag gccgtggtcg gcaaaataca
    30841 tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa ctatctgtgt
    30901 aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac acagcgctgg
    30961 attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga acaatcagtt
    31021 tcttttgctg cgtcttttgc tgtaacgctt gcgataaatg cggcaacaac aattcatcaa
    31081 atcctctgac atagactgtt tccatggcgc gcatggcgat actggtaact gctgtaccat
    31141 tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct tgattagcca
    31201 tattaataat gttgtccgga taattatgaa tgtcatgact caaaacagaa tcagcagtta
    31261 aagaaagggg aaccgacagc gcagtgtaag gtgcaccgct tattgcttga ttaaacagtt
    31321 tgatctgctt tctttgtgct tcgacttgcg gtgtcgtaga gtgtgtatat ccatacaaag
    31381 acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca atacctgtat
    31441 ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat ctttgatgct
    31501 ctttcgctgc taaagcaaaa taattgagat tgaaaaacgg cgtataggta gcaaatcgcg
    31561 acgctagtat atatggactg aacgcctctt tattttttgc atctttataa gcaaattgac
    31621 aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc actttttttg
    31681 tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat aaaaaggcaa
    31741 aagcacatag ataagggata tacattccca gcattttgac aacttcatcc ggatcactct
    31801 gcagcacact aatcgcaaat ccatcattga aagttgtacc aaagatacac catgaataga
    31861 ggctaatact catatctgcc gcagtcagta acacaaatgg tgcggcaata agcaggcgaa
    31921 gcggaaggga tggtatacgt ttaacaacca ggatggtcag aacgaagaac agcaatcgct
    31981 gaaccatatg cgctccggaa gaagcgacta agttcgtaat accaaaaata ataacacaaa
    32041 gatacaacaa ccaatgagat ttaaaacttt taacaaattt gttgaatact gtcatccaga
    32101 aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta tatcagacat
    32161 gtttttcaac tctaatccat atcagaccga aacactgcta gattttttct ttttaaatga
    32221 tgcggccatt gcgttctcag taattaaata acattatcaa tgcgtctcta aacattacgc
    32281 agaacgtagc catcagtagt tatactgaca ggggcaggat ggtttatcga gaagtaaaca
    32341 tatccgtatt cgtaccggaa atggagttga cacaataaag tgccaattat gtcagcagaa
    32401 gggaaaagct tataactaag gcataccggc accatcgttt caaggtacca gctacgagta
    32461 aaacaactgg acgagataca gatacctgac aaccattcct cagaccagga ccaaaacgaa
    32521 aaaagacgct tttcagcgtc tttctggaat ttggtaccga ggacgggact tgaacccgta
    32581 agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac
    32641 ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggctgagt
    32701 ttgttcggtt ttcgccggcg cactcaggtt ttcccattcg ctacctttat tggttttgtt
    32761 gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca gcagcgccgt
    32821 catgcgagtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc
    32881 tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag
    32941 gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca acctgttcct
    33001 tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggaatattta acctttccca
    33061 ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttattgca
    33121 ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa aaaggcgaca
    33181 atcgccgcct ttttagccag ccatttatcg ttttaaacgg cttttactgc atcggcgatg
    33241 cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac
    33301 ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc cgcttcaact
    33361 tctgcggtca cagctttgac tgattcatgc tccaacggat cgccgctacc tgcggtgtaa
    33421 cgcacattaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc
    33481 atatggttac gcgccatcgc cgccagcacc tgcaagccag caacaatgcc gtcaccggta
    33541 gtggttttat ccagcaggat cacatgaccg gagttctctg caccgatacg ccagcctttc
    33601 tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaatacct
    33661 aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac agcaccacca
    33721 cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg
    33781 actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata
    33841 ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc
    33901 acttcggcat tgatatttac accgttcggc tcacaaccga tagcgataac gttcgccccc
    33961 agttcacgca gcacgttcgg tgcgatgtga taagtcgcac cgtttgcaca atccaccaca
    34021 atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatataa
    34081 cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc aacacagctg
    34141 atctcttttt ccatttccgc ttcgatggct tcttcaactg catccggcag cttagtaccg
    34201 tcaatagaaa agaatttaat gccgttatcg tagaatgggt tatgcgatgc agaaattaca
    34261 attccagcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg
    34321 ccagtgaaga gcgcggaaag accggcagcc gcaagacccg cttccagtgc tgactccagc
    34381 atatagccag aaatacgcgt gtctttacca ataataatct tacgagagcc gtggcgcgcc
    34441 agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc
    34501 cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt
    34561 ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc
    34621 atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc
    34681 gctcaggcgc tcggacggcc ccacgttcag caattgccca atcatcgatt ttcgtgacat
    34741 acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata
    34801 gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc
    34861 gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa
    34921 gacatcgtca tacttcggcg cttcctgcat ggtttttgga tttccctgca tatgcatcag
    34981 acaaaccggt aaaccggttt ctgcagccgc ctccagagca ccaggttcgg aaagggagcg
    35041 gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga
    35101 tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac
    35161 acgttgcaac tcttcttcaa cgcaaacttc cgccgcccct gggcgcgtgg actcgccacc
    35221 aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca ccgcatctaa
    35281 cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat
    35341 tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc
    35401 cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata
    35461 cagtcatctg atacgggaac ttacttgtcg cctaactgct ctgacatggt gttacccggg
    35521 ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa
    35581 ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc
    35641 aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca
    35701 tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc
    35761 agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc
    35821 gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag tggacccaat
    35881 ttctcagaga agccccactg ggtcaccatg ttacgtgcca ggttggtcgc aactttaata
    35941 tcgttggacg ccccggtaga aacatgttcc ggtccgtaga tgatctcttc cgccagacga
    36001 ccaccgtaca gcgtggagat ctgactttcc agtttctgac ggctggcgct gattgcgtcg
    36061 ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcactttg
    36121 tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc ctcgtggtaa
    36181 gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg
    36241 attttgtctt tcgctttctc gaactcaacc atcgaaacaa cgcgtttgtt gccacgagca
    36301 gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa accaggagta
    36361 ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact
    36421 ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg acggtcgaaa
    36481 cggccaggac gcagcagggc tgggtcgaga acgtccggac ggttagtcgc agcgataacg
    36541 atgatacctt cgttaccttc aaaaccatcc atctcaacca gcatctggtt cagagtctgt
    36601 tcacgttcat cgtgaccacc gcccaagcct gcgccgcgct ggcggcctac ggcgtcgatt
    36661 tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca
    36721 cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac
    36781 ggaactttcg cttcgcctgc aattgctttc gccagcaacg ttttaccggt acccggagga
    36841 cctaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctca
    36901 cgcagatact caaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca
    36961 gcaaaggtgg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt accaaacgac
    37021 atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc
    37081 agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc
    37141 ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga
    37201 accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg
    37261 ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc
    37321 accttacggc cattagactc gctgggccca aagctctgga atactgacat cagcacaacg
    37381 gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat
    37441 tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt
    37501 ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt
    37561 gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt
    37621 agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct
    37681 aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc
    37741 accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg gactttgctg
    37801 tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga
    37861 aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg
    37921 ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca
    37981 accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag
    38041 gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc
    38101 tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg
    38161 gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag
    38221 atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag
    38281 tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct
    38341 tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca
    38401 tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct tgatcgtgga
    38461 agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct
    38521 ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac
    38581 acacattcgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc
    38641 tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata catcaacatc
    38701 ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc
    38761 gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat
    38821 aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg
    38881 ataagtctgt tcttcgtcag aatcgagatt cagcaccgtt acggtagcac caaaaataac
    38941 gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca gcttggcttc
    39001 gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc
    39061 gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg
    39121 gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat
    39181 cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca
    39241 ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc
    39301 gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat tttaccctgg
    39361 agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat
    39421 tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg
    39481 tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac tccccgcagg
    39541 tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta ttgattacca
    39601 cagtcagcag atggcgctac ccgccagtac gcagaaagtg attactgcgc tggcggcgtt
    39661 gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag gcaatgtgga
    39721 aaacggcgta cttaagggtg acttagttgc gcgatttggt gccgatccga cgttaaaacg
    39781 tcaggatatt cgcaatatgg tcacgacttt gaaaaaatct ggcgtcaacc aaatcgatgg
    39841 caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg
    39901 gaatgacatg acacaatgct ttagcgcacc gcctgccgcc gccatagttg accgcaactg
    39961 tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta tacgcgtggc
    40021 atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg gttctgccga
    40081 agcgcaatac tgcgaactgg atgtcgtacc aggtgacctg aaccgcttta cgctaacggg
    40141 atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg atggtgccag
    40201 ctatgccggg gcaattctga aagatgagtt aaaacaggcg ggtatcgcct ggagcggaac
    40261 actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc
    40321 cccgctgcac gatctgctta agattatgct gaaaaagtcg gacaacatga ttgccgatac
    40381 ggttttccgc atgatcggcc atgcgcgctt caatgtgcct ggaacatggc gggccggatc
    40441 ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatatcggaa acaccattat
    40501 tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt
    40561 gctgcaatat attgcccaac acgacaatga acttaacttt atctccatgc tgccgctggc
    40621 gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggaaa
    40681 agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac
    40741 agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag tagaaccggc
    40801 ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt ataaagatat
    40861 ttatcagaat aattagccta aagagaaacc ccggcacatg gccggggttt tgctgattaa
    40921 cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcgtc atcccagtcc
    40981 tcatcatcct cttcagcaat ctcttcaagc tgctgacggt gataatcatc ccacatgaat
    41041 tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc aatgataaag
    41101 gtcatcacat cccagcagag atctttcacg cccagtccgc tcgccgcaga gatcagataa
    41161 tatttatctt cccagcccag cgcttcagcg atcgctttcg ctttctcttc ggcttctgcc
    41221 ttatccagca gatcgatctt gttgaaaact aaccaacgcg gtttcgccgc cagatcctgg
    41281 ctgtattttt ccagctcact gataataata cgcgcgtttt caaccggatc ggtgccgtca
    41341 atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg cttcaggaag
    41401 cgaatgccca gacctgcgcc ttctgcagcg ccttcaatca gccctggaat atcggcaaca
    41461 acgaagctct tttcgttgtc cattcgcacc acgcccaggc ttggtaccag agtggtaaac
    41521 ggataatccg ccactttcgg tttagccgcc gatacagcac gaataaaggt cgatttaccc
    41581 gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag cagcagctca
    41641 cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg tacggttaac tgacgatttg
    41701 aaacgagtgt tgcccagacc gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt
    41761 ttggtcatat cacccatggt ttcaccagta ccctggtcga ttacacgcgt acctaccggc
    41821 actttaatcg tgacgtcttt accgcgctta cctgtacagt cgcggcttgc accgttttga
    41881 ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt caggttctcg
    41941 tcggcttcca tccatacgtc accaccgtca ccgccgtcgc cgccatccgg gccgcctttc
    42001 ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc tgcaacgacc
    42061 agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc gcctgcgcgg
    42121 ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg accaatggcg
    42181 gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag gtttaacatc
    42241 ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac
    42301 agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc
    42361 gccagggcgc catatcctac caatgtattc agtccgcaaa aaattaaaca ggcgagctgc
    42421 cagtggctaa gctgcgctat cactccagga tttgccagag ggaagagcgc aattgtacat
    42481 aaagtgtaca gcaaaaacag gatctgcggt gaggccagcc gacgcaataa aaccttttgc
    42541 gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac tccccaggtg
    42601 taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat caccaggccg
    42661 ctcaggagca tcaatgcccc tacaacctga gtactgcgca ttttctcttt caggataaat
    42721 acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga agcggtcgga
    42781 ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc ggcggtcgcc
    42841 actgctaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg caaccgcttc
    42901 ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta aaacacgatt
    42961 gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc cccccagcaa
    43021 attgctgtgg tgagcgccaa aagaatgcca atgcctgcct gctgcttcat accgtattcc
    43081 ctgcaaagcg catatgcgga ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg
    43141 taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg aaaaacttat
    43201 tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa tttcacttta
    43261 ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg
    43321 aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc accaccgaaa
    43381 cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc
    43441 tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca gtaattttca
    43501 catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga cgacgaaact
    43561 taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg attacgccgc
    43621 catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc agcacttcag
    43681 cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg gtctgacctt
    43741 cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa aactccgctt
    43801 ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata gggcgcgaat
    43861 attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac
    43921 aacttgtacg gtaacgttta tctgtgccat tttttcagta caatcaccct atattcctaa
    43981 ccataaaccc taagttgcct ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt
    44041 tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc
    44101 ggcaatcctt gagcagctta attccgacgt ccaactgatc aatcagttag gctattacat
    44161 cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct gtactggctg cacgagctgt
    44221 tggctatgag ggaaatgcgc atgtcaccat tgctgccctg atcgagttta tccacacggc
    44281 gactctgcta cacgacgacg ttgtggatga atcagatatg cgcaggggta aagctaccgc
    44341 caacgccgca tttggcaatg ccgccagcgt gctggtaggc gattttattt atacccgcgc
    44401 gttccagatg atgaccagcc tcggttcact caaagtgctg gaagtcatgt cagaagccgt
    44461 aaacgtcatc gcagaaggtg aagttctgca actgatgaac gttaacgatc cggacatcac
    44521 tgaagaaaac tacatgcgcg ttatctacag caagactgcg cgtctgtttg aggctgccgc
    44581 gcagtgttcc gggattctgg ctggctgtac gccggaggag gagaaaggcc tacaagatta
    44641 tgggcgctat ctcggcactg ctttccagtt gatcgacgat ttactcgatt acaatgccga
    44701 tggcgaacag ttaggtaaaa acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc
    44761 gctgctgcat gcgatgcatc atggcacacc agaacaggca cagatgattc gtaccgccat
    44821 cgaacagggt aacggtcgcc atcttctgga accggttctg gaagcaatga acgcttgtgg
    44881 atctcttgaa tggacgcgtc agcgtgccga ggaagaagca gacaaagcca tcgcagcgtt
    44941 acaggtgctc ccggacaccc cgtggagaga agcacttatc ggcctcgcgc acatcgctgt
    45001 tcaacgcgat cgttaatccc ctcccctcat cccgcgcagt gcgcgggatg attccagtaa
    45061 attccataaa acacttattc agctctataa ctcctgaaaa acagggctgt gtatctttat
    45121 atattctgaa tattcacact ctttacagga actttttaga gcaataggcc atcaggagta
    45181 tagtgatgct cgacagaaac agaagaagta ttctgaatga aagcgactaa tttaaggagt
    45241 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    45301 gcaagaaggg aacgtcaatg gcggcggagt ctcgcagaaa tggtttgagt tcctcaacgc
    45361 tggcgaacgc attatcacgc ccatggccga aaggagagat gattattgcg aaagccctgg
    45421 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    45481 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    45541 agccccgcga acggggctgc cagctctcag acaattactc gcctttcaca cgctcaatat
    45601 ttgcacctaa agcgcgtagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    45661 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    45721 cacgcagatc ggttgccata acctgtgcgc cagaaagttt ttcaacaccg tgacaaataa
    45781 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    45841 agcggttttc aaagaccgtt tcggtgatga atccggtccc ttctgccacc aggttcaaca
    45901 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45961 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    46021 ccgctccagc gtcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    46081 ggcagataat tttgccgcga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    46141 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    46201 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    46261 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    46321 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    46381 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    46441 attgttcgag gccagaaatg tgtagatcaa ccggacgtgc gccgatcgta caaccgccag
    46501 gcagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    46561 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    46621 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagc ttcattgatg
    46681 tatcgacgtc ttttagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    46741 cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    46801 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    46861 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46921 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46981 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcatgccg tcaaacaact
    47041 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    47101 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    47161 gttaataaat cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    47221 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    47281 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    47341 tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacattgttg ccctgctttt
    47401 tcgccagatc gataagatgg agcagcagtg ccagtccacc cgtatccacg cgagacacac
    47461 ggctaagatc gatgctggta atccccttca ccgcttcctc acgtatttcc caaagcggta
    47521 gcaaaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    47581 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    47641 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    47701 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc agttgcccgt
    47761 ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat tcgggtcaat
    47821 aatggtaacg cgaatcggca caatggtttt atcacccagc ggctgttctg gcgcaatctg
    47881 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47941 gaaagcggca aagtaagcat cacgttgagc tggggtcgca ctcttgtaat actggcccag
    48001 caccagcgca ccggcgtatt tcacctgtac gtatggcaac agttcctgat caacaatggt
    48061 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    48121 tttctgcgcc gcctcgtcca tcagcttata cggattggtc tggtctgccg cggttgccgc
    48181 actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    48241 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    48301 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    48361 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    48421 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttcccc
    48481 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    48541 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    48601 ccggcccaca acaacgccac caatgctgac cggagagcgg gctttcaggc cgccaatgtt
    48661 atcgaacgtc gcataaagcg tataggtcgg ttcagtacgt atagacgtca cgttcgctgc
    48721 cttcaggcaa acaaacagcg ccgcaagcag tgctgctaat aaaaagatac ccacccaaat
    48781 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    48841 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48901 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48961 cgaacaccac gctcttaatc agacagttga cgagatccat acgccagtcg acggcatttt
    49021 gcattgccga ccagaagaac ccactatcaa tgcctttcca actgacgccg accagcgatc
    49081 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataactccag
    49141 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    49201 agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa ccagcacgcc
    49261 cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    49321 gcatacccag actggtttcc gcactataag tggtcagaac cagataacct tgcagcccca
    49381 acaccattcc gatgaacacg ccagaaacaa caataatcag catcgacagg acgccgacat
    49441 tatagagctg gcgcaccagc agcggtgcat gtttgcgaaa ttccggtttg ccgaccagcg
    49501 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    49561 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    49621 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    49681 tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cactaccatg agcgacaatt
    49741 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    49801 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagc
    49861 acgcccatgg taatgggatc ttgcccaaca aagggctcat caaacatgat gagatccggc
    49921 tccagcgcaa tcgcacgtgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49981 ggcataagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    50041 tgcaacaatg gcgcgggaag ttgggtatgt tcccgcagtg gataggcgac gttgtcaaat
    50101 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttgcgcact
    50161 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag aatctcacca
    50221 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    50281 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    50341 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    50401 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    50461 aacccaacat atttacagaa tattacctgc cgtggttagc gaaagctggc atttgtttta
    50521 ctttttagcc gcataaagtc aaaattaaga atctgttacg gctttctgaa aatcttcagc
    50581 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    50641 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    50701 ttgccgaacc tttggcatcc cgccgttgat catcggcatg acggtggtca gtattggtac
    50761 atcgttacca gaaatcatcg tctcgcttgc cgcgtctttg cacgaacaac gcgatttagc
    50821 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    50881 gctggttcgt ccttttaccg tccattctga tgttctacgc cgtgaattac ccttaatgtt
    50941 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gcagcgatgg
    51001 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    51061 ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    51121 aggtggattg cccgtcgcat ttttatggct cggcatcgca ctgatcatta tgccagtggc
    51181 cacgcggatg gtagttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    51241 gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg caaccgcaat
    51301 agcaggggtt cgcaaaggtg aaaacgacat tgcggtcgga aatatcattg gcgcaaacat
    51361 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag agattgatcc
    51421 actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    51481 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    51541 tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    51601 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    51661 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    51721 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    51781 aatcggggca catcgggcgc aaaatggcag caacatttgc cagcactggt acaccttcat
    51841 ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc ccgcaggatg
    51901 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    51961 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    52021 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    52081 caccgaccag cagcacaacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    52141 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    52201 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    52261 ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta
    52321 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    52381 gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    52441 cggggggaat acgcgtgcgc ccaggcattc tggccgttga ggcactgaac ttaatgcagt
    52501 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    52561 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    52621 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc
    52681 agagaacatt cgtctgctga tcctcgacgt tgacggcgta ctgtcagatg gcctgattta
    52741 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    52801 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    52861 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    52921 gatcgccttt agcgatctgc tggaaaaact ggcgattgcc ccggaaaatg tggcttatgt
    52981 cggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    53041 cgatgcgcat ccactgttga tcccgcgcgc cgattacgta acgcgcattg ctggcggtcg
    53101 tggcgcagtg cgagaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    53161 caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct atcactggcg
    53221 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    53281 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    53341 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    53401 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    53461 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    53521 gaagtcaatg cactcgtgcc ggactctcaa cttcgcagaa tcacgacgga taacgcgcag
    53581 atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    53641 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    53701 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    53761 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    53821 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    53881 taccggtaat gttatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    53941 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggctacggta aaccggcaac
    54001 gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc agatgcacta
    54061 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    54121 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    54181 cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagttgcagg acaaaaacaa
    54241 taaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    54301 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    54361 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    54421 ctacatggtt gttggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    54481 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccgcagga
    54541 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    54601 tgacgacttg tctgctgaac aacgtgaaga ccgtgcgaac gagctgatgg aagagtttca
    54661 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    54721 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc
    54781 cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgagcacc tgcgcgacag
    54841 cggcctgggc gtactgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    54901 cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag aaatcttaca
    54961 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    55021 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    55081 cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct gttgcagttg
    55141 tcgacgctgg aacttcagca agagctacag caggcgctgg agagcaatcc gctgcttgag
    55201 caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga aacactggac
    55261 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    55321 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgatgacgag
    55381 ctgccggtct atcagggcga aacgacgcag accttacagg attacctgat gtggcaggtc
    55441 gagttgacac cgttttccga cactgaccgc gcgattgcta cctctatcgt cgatgccgtt
    55501 gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    55561 gagattgaca tcgacgaagt tgaagccgtc cttaagcgga tccaacggtt tgatccagtc
    55621 ggtgtggcgg caaaagatct gcgtgactgc ctgctgatcc aactctccca attcgataag
    55681 accacgccat ggctggaaga ggccagactg atcattagcg atcatctcga tctattagcc
    55741 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    55801 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    55861 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggccactg gacggtagaa
    55921 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    55981 aacgcgcgca atgatggtga tagccagttt atccgcagca atctgcagga tgccaaatgg
    56041 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    56101 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    56161 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    56221 tacctgcata gtccacgagg catttttgaa ctgaaatatt tcttttccag tcacgtcaat
    56281 actgaaggcg ggggtgaagc ctcttccaca gcgattcgtg cgctggtgaa gaaattaatc
    56341 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    56401 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    56461 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    56521 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttacagccaa
    56581 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    56641 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    56701 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    56761 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    56821 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgaacctg
    56881 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    56941 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact gccagagcaa
    57001 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tggcgcctca
    57061 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    57121 tattgccatt ccgcatggca aactggaaga agatactctg cgtgccgttg gcgttttcgt
    57181 tcagctcgaa acacctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    57241 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    57301 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    57361 gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa
    57421 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    57481 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    57541 ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg atcgagagat
    57601 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    57661 ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg atgccgaccg
    57721 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    57781 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    57841 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggcag aaatgctgcg
    57901 tacccgtctg ttgggtaaac gtgaacgtga actgaccatg gtctttgagt ctttcggctt
    57961 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    58021 acactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    58081 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    58141 gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg gttgtaccgg
    58201 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    58261 taaaaacgtc cagtcacgcc atcggacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    58321 actgttgaaa ttacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    58381 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa
    58441 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    58501 gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct ctttaactct
    58561 ggttttgatg aagattaatc ttcatcactt tgacattcaa atcccttcaa gctcccccgg
    58621 caataagaaa agcgcgtact gaaatcgtta tcaatcaacg tcgattgtac atttcgtgcg
    58681 ctttttactt tcagaagaac cttaagaaaa ccttaagaag cattgtttag atttcgttta
    58741 agttaatcga ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct
    58801 tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag
    58861 atgcccaacg ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc
    58921 gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac
    58981 cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg
    59041 ccgatttcga cggcaagcct tccattacgc tgaccgaatt tgcagaacaa tgccgttacg
    59101 aagaagacat tgcacaactg cgccagttac tgaaacagct gaagcgttat ttgcaggata
    59161 accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt
    59221 cagaggttat ccccgtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga
    59281 cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc
    59341 aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg
    59401 ccctcacttc gcgcgaagct taatcaagct ggtgttgttg cataaacggc tcaccgccta
    59461 actgatacat ctgccgtaaa atccacgcct gacggctacg cacatagccc gatggcgcgg
    59521 agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg
    59581 taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac
    59641 cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg
    59701 tttctatccc cagcgttaat ccggcttcca gcccttttcg aacccagcta cgcccatccc
    59761 ataaaaagag atttttggct gtctgctgag aaatcgttga agcaccgcga atacggtttt
    59821 cattgcgctc gttgtgcgcc agggcttgct caatggaagc gacatcaaag ccccagtgct
    59881 caggaaattt ctgatcttct gcggcaatca ccgccagtcc catccacggc gagatttgat
    59941 ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc caggcgctga
    60001 cttgccgctc gaccattacc gctgagaaag gaacaggcgc aacgctaaac aacgcgatgc
    60061 ccccgcccca gaaaatggcg aggacaacca ttaaccgcag aagaaaacgg cgaacgaaac
    60121 taaacaccgt taagcggctt ttactcattc agccagaacc agcacgcggg aaaccagctt
    60181 atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc atatatgccg gggtggtgac
    60241 aatcttattg tcttcatcaa ccacgatatc atcgacagga cacggcacat gctccgcgcc
    60301 catctcttcc agcacttctg cggtatcgat atcagtaccg atggtcaaac gtagcgggaa
    60361 atcgaaaatt ttcggcagta tcgccggtgc aatacacata aaaccaagcg gttttccggc
    60421 ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt cgctaccaag
    60481 actggcaaaa ttgcttaaat tcttcgccgc gccaaaaccc cccggcacaa tcaacgcatc
    60541 cagttcagcg gcatcggcct gggccagagg acggatttca ccgcgcgtta ttcgtgccgc
    60601 ttcaatcagc acattgcgcg tttccgtcat cgcttcacca gttaaatggt tgataacatc
    60661 aacctgctgc ttatccggtg caaagcaaac cgcctgcgca ccgctgcgtg aaatagctaa
    60721 caacgtcaat accgcttcat gaatttcaga accgtcatag acgccgcatc cgctcagaat
    60781 tacgccaatt ttcttcattg tgatcttcct ttcgcaattg actgaaacac attaattttt
    60841 ttaataaaaa tgctacgcat cacacattta actgattcat gtaacaaatc atttaagttt
    60901 tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat
    60961 aatttacggc gcagccaaga tttccctggt gttggcgcag tattcgcgca ccccggtcta
    61021 gccggggtca ttttttagtg gcttttgcca cccacgcttt cagtacttct acgtcgtgac
    61081 gccactcttc tttcatctct tcaatccatt caccgacgtt atcttcccag gcgggaaggt
    61141 caggagactg aatttgctga cccagctgtt gcagatggcg taaccccact gaccccgccg
    61201 cacctttaat tttatgccct tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg
    61261 actccagcac gctgacatag cccggcatca ttctctcaaa caccgctaac ccgtcggtga
    61321 tcagcttcgg tcctacaagt tcgagatact gttccagcat gggaatatcg agcaatgctt
    61381 ctgatttact gttctcttct gtcgtcaccg tactctcctc atcatcctgg gtatcccaga
    61441 atttcttgat catcgcggtt agcgccggaa cagaaagcgg cttactcagc acatcatcca
    61501 ttccagcatt gaggtactct tgcttgtctt tcagcacgtt agcggttaag gccaccagcg
    61561 gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg agagatatcc agcccggtca
    61621 tatctggcaa ctgaatatcc agcaatacca ggtcgtattc gcccggttta aacatctcca
    61681 gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc taatttttcc agcacagaac
    61741 gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag cacattcagc gccggtaaag
    61801 gcatatcgtc ttcatcaaac gcatcatcaa cctcttctgc taccgacggt gcgtggatcg
    61861 tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg ccgcccatat
    61921 ttttcgccag acgacgagaa acggccagac caataccggt gccggtggca ggtttaccgc
    61981 catgactgtc tttcacctgg taatacatgg cgaagatttt atccagctca tcctgcggaa
    62041 tgccgatacc agagtcttcc acttcaaaat gcagcatatc gccttcatcg tagcgcacgc
    62101 gcacggtaac ctggccttgc tgagtgaatt tgacggcgtt actgatgagg ttccacaaga
    62161 tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg taatggcagc gtcggctcca
    62221 gattaaagcg caatcctttt tgttgcgcct gcaatgcgga gagattttcc agatcggcaa
    62281 ggaagctggt gaaatcaacc ggctgattat caagctggac cttgcgccgt tccatcttat
    62341 ccatgtcgat aatatcgttg aagatattcc ccagcgtgac agccgaaaca tggatggttt
    62401 tgagatattt ttcctgctcg gcggtgagtt cagtatccag cagaatgcgg ctcaggccga
    62461 cgataccgtt cagcggtgta cgcaattcgt gactgatggt ggagataaac gtcgttttgt
    62521 cgcggctggc gcgttcaagc gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa
    62581 agcccatcaa accgtgacgt ttacccacgc ggtcgtagta cggcacttta cggatttcaa
    62641 agcaggcttt gcgtccgtcg ggataatcca gccactgctc ataggtcagt gacacattat
    62701 gacggaacac tttttcatcg gtttcaatga ctttcgcagc ggcttctggt gagtaaacat
    62761 cagcaggttt caggtgaacc agttgttttt cgctttttcc ggtcagcagc tccatcgcgc
    62821 ggttacagcc ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcgt
    62881 caaggaagga acgtaagaat gaggattgct gctcgagctg aatttgtgtc tcttcgcgct
    62941 ctttgatttc aattttcagt tggccgaagg tttcctgcag ttctgcttcc gctttttcac
    63001 gaacggcgat ttcctgattt agctgggcaa tattatcttt taactgaacg ttgaggctca
    63061 aatcgcgctc gcgcatctcc tccagttttt gcaccagtcg tgacagacgt tgtcgtgact
    63121 cctccagttg ctcgacgacc accgatagaa agtagaccgc ccacggcgta atcagcaaac
    63181 caaagaagat agaacgaata acatcaatgc tttcgacctg accatgcagc accatggtta
    63241 ccgccatttg taccacaatg gcaagaacga cgagggccag cgccagcaac attgagaagc
    63301 gcaccagacc taacttcatc atcaggtcaa cataatactg cgccagcaga cgaatttgct
    63361 tcattaggga attccttcac gacaacctgc cccaataata cccaattctg acagttacgt
    63421 tataaattgt gtgagaaatg cggaattccc ctcaatggcc tacgccgttg gaggtagcca
    63481 cggacgtccc agcgccgatc cctgtacgcc atgttcattc agataacgat ccagctcgac
    63541 cattcccgtc catcgatttt cgcaccacag cggagcaagc agcgtcgggc gacgggcgct
    63601 ggcggaaata cggtggtaga taacttccgg tggcgtatgg cgaatcattt ctccggcagt
    63661 gagcgtgtaa tcctccagtt caattccgtt caaacgcccc gcttcccagg ctttcgccat
    63721 aatgctgcct ttcactatat gcagcggatg cagctttatg ccgtccacgc cggtttcaac
    63781 cactcgctcc agcgtttgca aacattcagc ctgcccttcg ccaggcaggc caacaattaa
    63841 gtgagagcac accttcagcc cacgctcacg cgccagctgc gtcgtgcgct gatagcaggc
    63901 aaaatcatga ccgcgattta tgcgatgcag tgttttgtcg tgggcggttt gtagccccag
    63961 ctccagccac acttcgtagc cctggtcctt atattcgcaa agcagatcca gcaccgcatc
    64021 cggcacgcag tccgggcggg taccaacaca caaaccgaca atattggcct ggctcaccgc
    64081 ctgctgatac atcgaacgca gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa
    64141 gtaggccaga tagcgtttag cgcggttaac taaattcgcc tggtgcgcca gttgctcggc
    64201 aatggaacga tgctgctgcg cttcatcggc aaacgaggca acattacaga atgtgcagcc
    64261 gccacgcccg atggtaccgt cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt
    64321 atgcaccttt tgcccataac gacgggtgag atcaccacca aacatattga ctaatttctg
    64381 taactgcata atctgataga ccgcgccttg aaaagaggcc aaagcctgcc atttttagcc
    64441 caattcggcg atgacctgga tcaatcgtcc cagcctgctt ttatcaactg cataaccaat
    64501 caaaattacc gaaatttcat gcacaatcac ataaatcact gttgcttatc ttgtgtcaga
    64561 tttttttatc ttcttatgga ttttaggcaa aaacagtagc atgaaacgtt attaccaatt
    64621 aaggcagtat aaaatgctgg ctttctcatc aattcaaggc aggataaggg ttaacacgcc
    64681 tttatgacag tcaggaattg gctgtttctc taacgacacc ccttttagcc ttaaatagaa
    64741 aatccacttt aatttcatac aattaataaa gaattttgcg ctaaagcaca tttctgtacc
    64801 aataagcttg ccatttgacc tgtatcagct ttcccgataa gttggaaatc cgctggaagc
    64861 tttctggatg agcagcctgc tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg
    64921 tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg aggtgcgcgt atgacacgca
    64981 aaccccgtcg ccacgctctt actgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc
    65041 agagcctggg ggaggttcac gatatgttgt acgataaatc ccttgagagg gataactgtg
    65101 gtttcggcct gatcgcccac atagaaggcg aacctagcca caaggtagtg cgtactgcaa
    65161 tacacgcact ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt aaaaccggtg
    65221 acggttgcgg cttgctgtta caaaaaccgg atcgtttttt tcgcatcgtt gcgcaggagc
    65281 gcggctggcg tttagcaaaa aactacgccg tcgggatgct cttcctgaat aaagatcctg
    65341 aactcgccgc tgccgcacgc cgcatcgttg aagaagagct gcaacgcgaa acgttgtcga
    65401 ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct gggtgaaatc gccctctcct
    65461 ctctgccacg cattgagcaa atttttgtga acgccccggc aggctggcgt ccgcgcgata
    65521 tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag
    65581 acttctacgt ctgtagcctg tcgaatctgg tcaatattta taaaggtctg tgtatgccgg
    65641 cggatctgcc gcgcttctac ctggacctcg cggacctgcg tctggaatcg gccatctgcc
    65701 tgtttcacca gcgcttttcc actaacaccg taccgcgctg gccgctggcg cagccgttcc
    65761 gctacctcgc gcataacggt gaaatcaaca ccatcactgg taaccgccaa tgggcgcgtg
    65821 cgcgtaccta taaattccag acgccgctga tccctgacct gcacgacgcc gcgccgttcg
    65881 tcaacgaaac cggctctgac tccagttcga tggataacat gctggaactc ctgctggcag
    65941 gtgggatgga tatcatccgt gccatgcgtc tgttagtgcc acctgcctgg cagaacaacc
    66001 cggatatgga cccggaactg cgtgccttct ttgactttaa ctccatgcat atggagccgt
    66061 gggatggccc ggcaggcatc gtgatgtctg acggtcgttt tgccgcctgt aacctcgacc
    66121 gtaacggtct gcgtccggcg cgctacgtca tcaccaaaga taagctcatt acctgcgcct
    66181 ctgaagtcgg tatctgggat tatcagcctg acgaagtggt cgaaaaaggc cgcgtggggc
    66241 caggcgaact gatggttatc gacacccgca gtgggcgtat tctgcactcg gcagaaaccg
    66301 atgacgatct gaaaagccgc cacccttata aagagtggat ggagaaaaac gtccgccgtc
    66361 tggtaccgtt tgaagatctg cccgatgaag aagtgggtag ccgcgaactg gacgacgaca
    66421 cgcttgccag ctaccagaaa caatttaact acagcgcgga agagctggat tcggtgatcc
    66481 gcgtactggg tgaaaacggt caggaagcgg tcggttcgat gggcgacgat accccattcg
    66541 ccgtgctctc cagccagccg cgcattattt acgactactt ccgccagcag tttgctcagg
    66601 taaccaaccc gccaatcgac ccgctgcgcg aagcgcatgt tatgtcgctc gccaccagta
    66661 tcggtcgtga aatgaacgtc ttctgtgaag cagaaggcca ggcgcaccgt ttaagcttta
    66721 aatcgccgat tctgctctat tccgatttca aacagctcac gacgatgaaa gaggatcact
    66781 accgcgcaga tacgctggat atcacctttg acgtcactaa aaccacgctc gaagcgacgg
    66841 tgaaagaact gtgtgacaaa gccgaaaaaa tggtacgtag cggcaccgtg ctgctggtgc
    66901 tctccgaccg gaatatcgct aaagatcgcc tgccggttcc ggctccgatg gcggttggcg
    66961 cgatccagac ccgtctggtc gagcaaagcc tgcgttgcga tgccaacatc atcgtagaaa
    67021 ccgccagcgc ccgtgatccg caccacttcg ccgtgttgct gggtttcggc gcgacggcta
    67081 tttatccata ccttgcctat gaaacgctgg gccgcctggt agacacccac gcgattgcca
    67141 aagattatcg taccgtgatg ctcaactatc gtaacggcat caacaaaggc ttgtacaaaa
    67201 tcatgtccaa aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag
    67261 cggtcggtct gcacgatgat gtcgtgggcc tgtgcttcca gggggcagtt agccgcattg
    67321 ggggggcgag ctttgaagac ttccagcagg atctgctgaa tctgtcgaaa cgagcctggc
    67381 tggcgcgtaa gcctatcagc cagggcggcc tgctgaaata tgtacacggt ggcgaatacc
    67441 acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc ggtacagagc ggcgagtata
    67501 gcgactatca ggaatacgcg aagctggtta atgagcgtcc ggcaaccacg ctacgcgatc
    67561 tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg
    67621 aactgtttaa acgcttcgat accgccgcga tgtctatcgg tgcgttaagc ccggaagccc
    67681 acgaggcgct ggcggaagcg atgaacagca tcggcggtaa ctcaaactcc ggcgaaggcg
    67741 gtgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg catcaagcag gtagcgtccg
    67801 gtcgctttgg ggttactccg gcgtatctgg tcaatgccga cgtcattcag ataaaagtcg
    67861 cccagggcgc gaagccgggt gaaggtggtc agttaccggg tgataaagtc actccttaca
    67921 ttgccaaact gcgctattcg gtgccgggcg tgacgctgat ctccccgccg ccgcaccacg
    67981 atatctactc tattgaggat ttagcgcagc tgattttcga cctcaagcag gtcaatccaa
    68041 aagcgatgat ctccgtgaag ctggtttccg aaccgggcgt aggcaccatc gcgactggcg
    68101 tggcaaaagc ttatgccgat ttaatcacca tcgcaggcta tgacggcggc accggcgcaa
    68161 gtccgctttc atcggtgaaa tacgcaggct gtccgtggga gctggggctt gttgaaaccc
    68221 aacaggctct ggttgccaac ggcttgcgtc acaagatccg tttgcaggtc gatggcggcc
    68281 tgaaaacggg tgtcgatatc atcaaagcgg cgattcttgg cgcggaaagt tttggcttcg
    68341 gcactggccc gatggtagcg cttggctgta aatatctgcg aatttgccac ctgaacaact
    68401 gcgccacggg tgtagcaact caggatgaca aactgcgtaa gaatcactat cacggcctgc
    68461 cgttcaaagt gactaattac tttgagttta tcgcccgtga aacccgcgag ctgatggcac
    68521 agctgggggt aacgcgtctg gtggatctga ttggtcgcac cgacctgctg aaagagctgg
    68581 acggtttcac cgccaaacag cagaaactgg cactgtcgaa gctgctggag actgccgagc
    68641 cgcatcctgg taaagcgctc tactgcaccg aaaacaaccc gccgtttgat aacggcctgc
    68701 tgaacgcgca gttgctgcaa caggcaaaac cgtttgtcga tgaacgccag agcaaaacct
    68761 tctggttcga tatccgcaac accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg
    68821 cccagacgca cggcgatcag gggctggcag ccgatcctat caaagcgtac ttcaacggca
    68881 ctgcaggcca gagcttcggc gtgtggaacg cgggcggcgt ggaactgtac ctgaccggtg
    68941 atgccaacga ctatgttggt aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg
    69001 ttggttccgc cttccgcagc catgaagcga gcattatcgg caacacctgc ctgtatggcg
    69061 cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc gtgcgtaact
    69121 ccggtgctat caccgtggta gaaggcattg gcgacaacgg ctgtgaatat atgacgggtg
    69181 gtatcgtctg tattctgggt aaaaccggcg ttaacttcgg cgcgggcatg actggcggct
    69241 tcgcttacgt tctcgatgaa agcggcgatt tccgcaaacg cgttaacccg gagctggtcg
    69301 aggtcttaag cgttgacgat ctggcgatcc atgaagagca tctgcgtggt cttatcaccg
    69361 agcatgttca gcataccggt tcccagcgtg gtgaagagat tctggcgaac tggtcaacct
    69421 tcgccactaa atttgcgctg gttaaaccga agtccagtga tgtaaaagca ctgctgggtc
    69481 accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga
    69541 gtcagaatgt ttatcaattt atcgacctgc agcgcgttga tccgccaaag aaaccgctga
    69601 agatccgcaa aattgagttt gttgaaattt acgagccgtt ttccgaaggc caggccaaag
    69661 cgcaggctga ccgctgcctg tcgtgcggca acccatactg cgagtggaaa tgcccggtac
    69721 acaactacat cccaaactgg ctgaagctcg ccaatgaggg gcgtattttt gaagcggcgg
    69781 aactgtcgca ccagacaaac accctgccgg aagtttgcgg acgtgtctgc ccgcaagacc
    69841 gtctgtgcga aggttcctgc accctgaacg atgagttcgg tgcggtgacc atcggcaaca
    69901 ttgagcgtta tatcaatgat aaagcgttcg agatgggctg gcgtccggat atgtccggcg
    69961 tgaaacagac cggtaaaaaa gtggcgatta tcggcgcagg tccggcgggt ctggcgtgcg
    70021 cggatgtcct gacgcgcaac ggcgtaaaag cgatcgtctt cgaccgtcac ccagaaattg
    70081 gcggcttgct gaccttcggt attccggctt tcaagctgga aaaagaggta atgacgcgcc
    70141 gccgtgaaat cttcaccggc atgggtattg aattcaaact caataccgaa gtgggccgcg
    70201 acgtgcagct ggacgatctg ctgagtgatt acgatgccgt gttccttggc gtcgggactt
    70261 atcagtcaat gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc
    70321 cgttccttat cgccaacacc aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt
    70381 tcgtcagcat ggaaggcaaa cgcgtggtgg tccttggcgg tggcgacact gcgatggact
    70441 gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg
    70501 aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa ggtgttgagt
    70561 tcaaattcaa cgtccagccg ctgggtatag aagtgaatgg caacggcaaa gtcagcggcg
    70621 taaaaatggt gcgcacagaa atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga
    70681 ttgtggcagg ttccgaacat atcgttccgg cagatgcggt gatcatggcg tttggtttcc
    70741 gtccacacaa catggaatgg ctggcaaaac acagcgtcga gctggattcg caggggcgca
    70801 tcatcgcccc ggaaggcagc gacaacgctt tccagaccag caacccgaaa atctttgctg
    70861 gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag
    70921 cggcagacgg cattatgaac tggctggaag tttaagcgag gtaacaatga attcgccaga
    70981 cagtgtctgg cgaatttata aaacgagcta tccaactaaa ttttaagcca ttaaatcagc
    71041 cttaaccttc actaccacct ttttaatctc accaggttcg ccaacaacgc atcccggttt
    71101 atgcggttct cctggcataa acacggcgaa cattcccggt ttcaggatga tggcttgctc
    71161 gttttcaatg gcgctgcaaa gctgataatc atcttcatgg tggaactctt cacactgacg
    71221 cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca gctggatatc
    71281 aatgtattgt tcgtgcaatt cggctttttt ctcaacgggc gattgagtat taaatgtcat
    71341 gacattcata aagatattgt cgccctgtaa ttcgtaacgg ccaggcgctt tttcttgcgg
    71401 tctggcagct aatgccaacg ttaacgcgtc ctgtaacgca ggatgtaatc cggcagacgg
    71461 taatgactgt acttcaccca tcatcataat atttctccct gggccaacag cgcagcccca
    71521 agtaaacctg catcgtggcg gtagtgcgcc gccagtaaat caacatgaaa tgccgctggc
    71581 tcctgcgcca gatacgtttc caccagcgcc agataccctt ctgccagacc aacgctgcca
    71641 ccgaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag ccttgcaagc
    71701 gtacgtgcgg agcggtgaat cagttgctgc gcctgctcat cgccctgccc ggcgcgcgtg
    71761 aaaatagttt tcgcattcgc accagccaac tccccctgcg ctgcagctgc aatgccgcga
    71821 ccagaagcaa tcgcttccac gcaacctgtg cgtccacagc cgcagactgg accatgcgga
    71881 tcggcaagcg tatgcccgat atgccccgcc agaccgccag ggccggtacg cagtttaccg
    71941 ccgctcacta caccgccgcc aacgccggtg gaaacagtga taaacaccat atcggttata
    72001 tcgccatcca gcgcctgata ctccgcccat gctgcggcct gcgcatcatt aatggcaatg
    72061 gtcggcaaat tggtaagttg ctccagcgtt ttgactaacg gaaagtgtaa caatccaccg
    72121 agattatgcg gattcagcgc cagcaagctg ccgtcgcgaa taattccggt tgaagcgata
    72181 gcaacccgct gcgcatgtgc ttgcaacgga gagactaatg cggataaggc atcacgcagc
    72241 gcctgcggag tctggctggc aggcgtagga agttctcggc gatcgcggat ctgcccgtca
    72301 gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccag tgtggtcata
    72361 gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg
    72421 cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atccgccgcc tgagcaggcg
    72481 tgttgtaacg cccttcggca atcacccggc atccggcgtc actcaacgtt ttcaccagcg
    72541 ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta gtgccaataa
    72601 tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag tcggtcatcg
    72661 ccagtaaacc gtgatggtga atacgtgcca gcagcgtttc aacaggcacc ggacgcggtc
    72721 ggtcagtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca tcaacatctt
    72781 caatataggc cgtgatgcgt accggagaat cctccagatc gcgcttcaca atcccaataa
    72841 tcggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttca atacgaatgg
    72901 caaccgcgcc cgcctgctct gccgctaatg ccatggcagc gacgatttcg ggtttatcga
    72961 gcgggctgtc cggaaccggc tggcaggaga caatcaggcc accgttagca gcgatttttt
    73021 gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg gtacagagac
    73081 cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc caaatggcac
    73141 ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcaa
    73201 ccaacgctga acacgagaag gcatatccag accaatcagc aggatcacca caaacgttaa
    73261 actaaacgag agcgagccaa gtgccgttcc cagatccaga cgttgagcga tcaacgcgcc
    73321 gaggattgga gctagtgcac cgcccagtgc gccaacgttg taggtaaagc ccagccccgc
    73381 cgcacgctgg tcggtatcga aataaccgcc gatcagtttc ggtaaaatcc ctgagatgcc
    73441 ctgaccaagc atttgctgga agaacagtaa caaaccgagc acccagacgt ttgcgccgcc
    73501 aatcgcaaat accggaataa tcagcagctg cgaggccagc agactacaaa cgtacgcttt
    73561 gcgggttccc agccagtcac cgaggaagcc accgacgcag caccccaccg ccgcgccaaa
    73621 gccactaaag aacagcacat tggcgacagt atgcgggtca taagccagat cggttttcag
    73681 atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcgaaca acacgaccac
    73741 catcagcatt acgcccgttg gccagcgttt gcccgtactc tgcaccataa agctgataaa
    73801 gattgcggcg cataacagcc cgagtacagc gacgattgcg gcattttgca ggttaccggc
    73861 gaagcagaac cacagcgcag tagccgccgc cagcgtcatt acgatattgg caatgcgatg
    73921 ttcaccacgg taaagaatgt ctaccatcgt gcgtactggt gctttacccc cgtgtttctc
    73981 tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga tgattggcaa
    74041 aatgccgata aagaacagcg cacgccagcc ccagaccgga accaccagac tatagacctg
    74101 agcggcaacg acggccccca ctgagaagcc tgaaatcaga aaaccactgg ctttgttacg
    74161 cagatgcttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt cacccgccat
    74221 ccccatgccg atgaccagac gagcgataaa catggtgatg tagcctggcg caaagccgca
    74281 ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac gacgcccgta
    74341 gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa aggctgcgga
    74401 gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt cggtgagtac
    74461 cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg cagcggaaaa
    74521 tgcccgccat tgtgcgcggt taagatggcg ataccacggg atattctggg ttgtagtact
    74581 cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta
    74641 cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaacaa ctcacccgcg
    74701 ctcttgcatc aactgctgag ccagcgcctt cagttctggc agatattttt catctaccgg
    74761 accaaacggt ttgcggcaca gcggcacaga aacgacatcc atataatgca ggacagtttt
    74821 cagcccacgg aatacgccag ttttgatcag taaatcaatg actttgttgc actcagtttg
    74881 cagtttctgc gcggtctgga tatcgccttc tttcagcgcc ttcacgatcc cctgataacg
    74941 ccagcccatg atgttgtaag tgctgccgat accgccgtct gcgcccgcca gaagaccaga
    75001 ggcgaagatt tcgtcgtaac cgttatagag caccagatca ggatgttcac gacggatctg
    75061 ctccatctga tagagatcgc cagaggtctg tttcagcgca cctacgccag gcagcgtaac
    75121 caaggtgttg atctgatcca gggtcagttt taccccgctc agggctggaa tgttgtacac
    75181 caccatcggc aacccatccg ctgaatcaat aattgcccga tagtgatcgc agtgttcttc
    75241 aaagctgaaa ggatagtaga acggcgtgac ggcggagacg gcatcgaagc cataacgttt
    75301 agccgatgcc gcaagttgtt ggctttcggc ggtgctgacg caaccgacat gggcgatgag
    75361 tttaatctta cctttcgcct cttcagcgac gatttccagt acctgttcgc gctcggaaag
    75421 gctttgtaca aaggcttcgc cggtcgaacc acccacgtac aaaccgtcga tgccctgctg
    75481 aatattgaac tgaaccaggc ggcgcagact cgctttatcc agtgcttgtt gttggtcaaa
    75541 aggagtcagg agtgcagcca ttacgccacg taaattcgtt gccataaata cctctgaagt
    75601 gatgcttgtc tgataaacga tataccttta tacctgttat accagatcaa ttaagcaaca
    75661 ccccatacag aaagcttata atgcgatctg cttcactaaa gtggcattat ttctttttgt
    75721 tggtggtctg accgaaggcg tgccaggtag cagagacgct gttgagatgc gattgcaacg
    75781 cgcgatcggc ttcgtcagga tcatgacggc ggatcgcgtc aacgatcgca atatgctgtt
    75841 gatagctaac gttgttatgc tcatgcagag cctgatcggt cacggttgga cgtgcggcga
    75901 ttagccagtc gagcagggca acgtggatcg ccatgaagat tgggttaccc gggatctccg
    75961 ccagaacgcg gtggaaatca acgtctgagc gaatgaatgc cgcgttgtta tccagcgact
    76021 gactgttgat ttccagtgct tttgccagca aatcgatttg ctcatcggtg gcgtgttcag
    76081 ccgcatagcg caccaggctg gattcaaaga acagacgtaa ttgttcgaaa tgggcaatcc
    76141 caccaggatg agaaaggaaa tctttcgcca tgccggaaag ctcgccgatg atggtgtccg
    76201 cagaaggacg tgagacgcgg gcacgttcgc cgttgtttat ttgcaccaga cctttgcgtt
    76261 ttaacgctgc cagcgcttcg cgcaccgaag gacgcccgac gttaaagaac gccatcagtt
    76321 cgcgttcaga cggtaattgt tcaccttcgc caaattcacg acggcggatc atctgttcca
    76381 gctcttcttc caccatttcg gagagttttt tacgcgccag cgggcggctg cgcaagttgc
    76441 gcccgatagt ggtagtagaa tcttcagctt gcggatcaaa tgcgttcata ggactcattc
    76501 aggaaaatgc gttagcggat aataacgacg ctatcgtagc acagtatcaa agtcaggcga
    76561 aaagatgaca gatagcaaaa caggccacat gggcctgttt tcattttact tcacaacgcg
    76621 taatgccggt cgaccaccgc gtggtggctg cggaggttca tcgtcaggat gagtttcatc
    76681 atcgtgatct ggcttgtcgc catcgataac cgacataacg gtttcgttgt ctgccgatgc
    76741 ctcttcatca ttcatgatgc tggcatcttc atcgtaggca gcttcaggct caaacatcgt
    76801 gcctgcgcca ttttcacggg cgtagatagc cagcacggca gccagcggca cagaaacctg
    76861 acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca tcattcgcca gttccagatt
    76921 gccgacagca cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat attccatagg
    76981 aacctgcacg ccagggagcg tcacatccac caccaggtgc ggcgtgagct ggttatccag
    77041 caaccactca tagaatgcac gcagcagata gggacgacgt ggtgttagct gtgacaaatc
    77101 catacagatt aactccggcc cagacgcatt tcacgttctg cttcagttaa agaagcaagg
    77161 aaagagtcac gttcaaagac gcgggtcata tagcctttca gctctttcgc acccgggccg
    77221 ctgaactcga tgcccagttg cggcagacgc cacagcagag gagcaaggta gcaatcgacc
    77281 aggctgaact catcgctcag gaagtacggc ttctgaccga agaccggcgc aatcgccagc
    77341 agttcttcgc gcagttgctt acgtgcggca tcggcttcag aagctgaacc gttgatgatg
    77401 gtgttcatca gcgtgtacca gtctttttcg atgcgatgca tgtacagacg gctttcaccg
    77461 cgagctaccg ggtaaacagg catcagtggc ggatgcggga aacgctcatc cagatattcc
    77521 ataatgatgc gagattccca cagggtcagc tcacgatcca ccagggtcgg aacgctctga
    77581 ttcgggttga ggtcaatcag atcctgaggc ggattgtcct tttccacgtg ttcgatctcg
    77641 aaacttacac ctttctcagc cagcacaatg cggacctgat ggctatagat gtcagtagga
    77701 ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga cagccatgaa aacctccagg
    77761 tatagtcaga atttttactg ctaccagcca ccaggtggcc agtcagaagt tgtgttaccc
    77821 agtaaggaac gactctcttt gttcgaaaat caaacaaaaa atgagcaata cccgacattt
    77881 gggcagaaaa ttggatgata gtttaccaga ttttgcgacc tttgtggtga gtcgatgccg
    77941 gaaatgggga aaaagagatg cgctttagtc tgaaatagtt gacttagtcc cttattggcg
    78001 atgtggtttt tgttttacct gtctgtcagg tggcagcaaa aagcaacttt ccatttttta
    78061 cgctgatgca gattttagcc ataaaaaaac ccgccgaagc gggttttttc gcaaattgtt
    78121 ttctgccgga gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg
    78181 cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg
    78241 cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat
    78301 cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc
    78361 aaccatgtcg accagttcca gcggctgacg aactaccatg cgagcagttt cacgaccgaa
    78421 gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac
    78481 gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta
    78541 taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc
    78601 cgcgtaaact ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc
    78661 tttaaccgcg atttcaatca cacgctcagg acggcgagca atcatctctt caaaggtcgc
    78721 ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt
    78781 gccggttaca gcaactttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg
    78841 cggagtgtat tccgctttgt gcttaccgcg caggcgacga gccagttcag tagccagacg
    78901 gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt
    78961 agctgtaaaa gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc
    79021 aaacgtcttc aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata
    79081 gcctatgcca gcacacaaaa agtttcggga aaaaaaactt tcttgtaacg tggggtcgca
    79141 ggattataga gaagtcgggg tcaaagatcg accccttttt gtgatttgtg gcaggtttta
    79201 ccccgccaaa tgctcgcgct tcaaatactc ttcgctttgc atctcttgca gacgtgacag
    79261 gcaacgctgg aactcaaact tcagccgctc gccctgataa atttcataca gcggcacttc
    79321 tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg
    79381 ccgcgcttcg ctctccatca accgcgtcat aactggtaca tcaaacaaca tgaccgtgtg
    79441 aaagaggcgt gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc acagcgtagt
    79501 aaaagaaacc gccagcgtct ggttctcgac gcccattgtc gccaatggcc gatggttgat
    79561 ttctaacgtc ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat
    79621 ttgcgcccgg gtttcatcgt tgaggggcga aagccacaga tgcgcctgag tgagtgtacg
    79681 caggcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
    79741 gattgcaggc aaaaaacgcg cacgttgcag gccatttcga taaagttcgt ccggcggaat
    79801 atttgacgtc gctaccaggg taataccgcg ggcgaacagg gctttcatca gaccgccaag
    79861 tagcatggca tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc
    79921 tgctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgca aagcagttag
    79981 ctcttcatgc acccgcagca taaaacggtg aaagtgcagg cgctgtttcc gctctcccgg
    80041 cagactttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
    80101 atataagcca cgcactggca tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac
    80161 ccgcgccatt agcccactcg tcctgggggc tggtggcgtg ctattgacga gttccttata
    80221 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc
    80281 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattgttatt
    80341 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
    80401 gcgatatgct gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
    80461 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
    80521 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
    80581 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
    80641 taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
    80701 attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc
    80761 cagtagcctg ctgccggaac tgtctgcgga agcaaacccg ttccgtaatc gtctggcaga
    80821 gtctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggcgc
    80881 atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttgggg cgcagccatt
    80941 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
    81001 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
    81061 agcaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
    81121 aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc
    81181 ccccctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
    81241 ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
    81301 tgatgattta ccggatcaac ctgcacaacc cttcgaaggt ttaggctccg gtgtcatcat
    81361 caacgccaat aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat
    81421 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
    81481 gagcgatatc gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
    81541 cgactccgat aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct
    81601 tgggcaaacc gctacctccg ggattatatc tgcgttaggc cgcagcggtc tgaatctcga
    81661 aggtctggaa aactttattc aaactgatgc ctccattaac cgcggcaatt ccggtggtgc
    81721 gctgttaaac cttaacggtg agttaattgg catcaacact gcaatccttg cgcctggcgg
    81781 cggtagcgtc gggattggat ttgccattcc cagtaatatg gcgcgaacac tggcgcagca
    81841 gcttatcgac tttggagaaa tcaaacgcgg cctgttaggt atcaaaggca ccgagatgag
    81901 tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt
    81961 gttgccaggt tctggctcgg cgaaagcggg cgtcaaagcg ggcgatatta ttaccagcct
    82021 caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcga ccaccgagcc
    82081 gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac
    82141 gctcgatacc agcacctctt cgtcggccag cgctgaaatg atcacgccag cgctggaagg
    82201 tgcaacgttg agcgatggtc agctaaaaga tggtggcaaa ggtattaaga tcgatgaggt
    82261 tgtcaaagga agcccagctg ctcaggctgg cttgcaaaaa gacgatgtga tcattggcgt
    82321 caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc
    82381 cataatcgcc ttgcaaatca tacgcggcaa tgaaagcctc tacctactaa tgcgttaatg
    82441 tcgtaaaccg ggcatcaggc tgacgtgtga tgtccggtta actcgtggta tgctgctgcc
    82501 gttccctttt ttaacgatgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt
    82561 ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg
    82621 ctttccactc cgcaatttga cagtaccgat gaaacgcctg ccagctataa tctggcggtt
    82681 cgccgcgccg cgccagcggt ggttaacgtt tataaccgcg gtttgaacac caactctcac
    82741 aaccagcttg agatccgcac cctgggatct ggtgtaatca tggatcaacg cgggtatatc
    82801 atcaccaata aacacgtgat caacgacgcc gatcagatca tcgtcgcctt acaggatgga
    82861 cgtgtttttg aagcgttact ggttggctct gactctctaa ccgatctggc ggtgctgaaa
    82921 attaacgcca caggcggttt accaacgatt ccgatcaatc cacgccgcgt accgcacatt
    82981 ggcgatgtgg ttctggcgat cggtaacccg tacaacctcg ggcagaccat tacccagggg
    83041 attatcagtg ccacgggtcg aatcggtctg aacccgaccg ggcggcaaaa cttcctgcaa
    83101 accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggcgaa
    83161 ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt
    83221 atcggctttg cgattccttt ccagttagcg accaaaatta tggataaact gatccgtgat
    83281 ggccgtgtga tccgtggcta cattggcatt ggtggacgtg agatcgcccc attacatgcg
    83341 cagagtggcg gcatggatca actgcaaggg atcgtggtta acgaagtgtc gcctgatggc
    83401 ccagcggcaa atgcaggcat ccaggtcaac gatctgatta tctctgtgaa taacaaaccc
    83461 gctatttccg ccctggagac aatggatcag gtcgcggaaa ttcgccctgg ttcggtgatc
    83521 ccggttgtcg tgatgcgtga cgataaacag ttaacattgc aggtcaccat tcaggaatat
    83581 ccggcgacca actaaggtca gcgtaaaaaa acaaaaaccg gagtctgtgc tccggttttt
    83641 tattatccgc taatcaatta cttattaacg aactcttcgc ccagggcgat atctttcttc
    83701 agcgtatcca gcataccttc cagcgcgttc tgttcaaatg cgctcagagt accgatagat
    83761 ttacgctctt ccacgccgtt tttacccagc agcagcggtt gagagaagaa acgagcgtac
    83821 tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt gttcgccctg cagtgcgcga
    83881 accagagaca gaccaaaacg tgcagctgcc tggcccatag acagggttgc agacccgcca
    83941 ccggctttcg cttcaaccac ctcagtaccc gcgttctgga tacgtttggt cagatcagcc
    84001 acttcctgct cggtaaaact aacgccagga acctgtgaca gcagcggcag aatggtaaca
    84061 ccagagtgac caccaataac cggcacttca acttcgcctg gctgtttgcc tttcagttcc
    84121 gcaacaaagg tgttggaacg aatgatatcc agcgtggtaa cgccgaacag tttgtttttg
    84181 tcataaacac cggctttttt cagcacttca gcagcaatcg caactgtagt gttaaccggg
    84241 ttagtgataa taccaatgca cgctttcggg caggttttcg caacttgctg taccaggttt
    84301 ttcacgatac cggcgttaac gttaaacagg tcggaacgat ccatacccgg tttacgcgct
    84361 acgcctgcag agataagaac gacatctgca ccttccagcg ccggagtcgc atcttcacct
    84421 gaaaaacctt tgattttcac agcagtaggg atatggctca gatcgacagc cacaccggga
    84481 gtcactggag cgatatcata cagagagagt tctgaacctg aaggcagttg ggtttttaac
    84541 agtagtgcaa gcgcctggcc aataccgcca gcagcgccga ggactgcgac tttcatccta
    84601 aactccttat tatattgata aactaagata tgttgctccg ctgccgcgac cttaatccac
    84661 aaaattgccg tttacaatga ccacatctca agaatgtgta gtcacgcaag tttagcgttt
    84721 atgcatttaa ttgccgtaat caggaaccta acttacgtaa ttaacagtca cttatgcatt
    84781 agcgcaacat tccaacaggt ggtgacaata taccctaccg ctcagccaaa acaatatcaa
    84841 tttgataaca attaatttac ttttaagcag aatttgcagg ccgtgacgca ggcatgtttc
    84901 tcaataacga aatttgataa aatcccgctc tttcataaca ttatttcagc cttcttcagg
    84961 gctgactgtt tgcataaaaa ttcatctgta tgcacaataa tgttgtatca accaccatat
    85021 cgggtgactt atgcgaagct cggctaagca agaagaacta gttaaagcat ttaaagcatt
    85081 acttaaagaa gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc aggagcaagg
    85141 ctttgacaat attaatcagt ctaaagtctc gcggatgttg accaagtttg gtgcggtacg
    85201 tacacgcaat gccaaaatgg aaatggttta ctgcctgcca gctgaactgg gtgtaccaac
    85261 cacctccagt ccattgaaga atctggtact ggatatcgac tacaacgatg cagttgtcgt
    85321 gattcatacc agccctggtg cggcgcagtt aattgctcgc ctgctggact cactgggcaa
    85381 agcagaaggt attctgggca ccatcgctgg cgatgacacc atctttacta cccctgctaa
    85441 cggtttcacc gtcaaagagc tgtacgaagc gattttagag ctgttcgacc aggagcttta
    85501 atctctgccc cgtcgcttct ggcggcgggg aaaatgttgc ttttgcctat caacccactg
    85561 ctttcccctg cgattcattt aacgaatagt gcgttttact gcgacatatc attcacacaa
    85621 tgaatacata tagtaaaaaa gcacattatg caaaattcat tatctaattg aaaatactat
    85681 aattaacaat aaatgatcgt gtttttaatt cttttttgtt attaaaattc acatttttaa
    85741 cacttagtat caactgaaac agttagcgtg gtattaatta gttcaataat tagtgtatac
    85801 ttgattttgt gatatgggtc acgaaacaaa ggcccagcta aaagattatg tcgaggtaaa
    85861 aatcatgaaa atcaaaacca ctgttgctgc attaagcgta ctttctgttc tctctttcgg
    85921 tgcattcgct gccgactcca ttgatgctgc acaagcacaa aatcgtgaag caatcgggac
    85981 cgtatccgta agtggtgtgg cgtcttcgcc aatggatatg cgtgaaatgc tgaacaaaaa
    86041 agcggaagag aaaggcgcaa cggcctacca gattactgaa gctcgtagtg gtgacacctg
    86101 gcacgctacg gctgaactgt acaaataaac cctcatcgtc ttgtccgacg atattgcccc
    86161 cggttcgggg gctttttttg cgctaatgac gaacattaaa acgcaaatgc ccttccagct
    86221 cttcctctgc ctcatcaaac agcaatatta acgcgccaaa acggcgacgc gttttctctc
    86281 ccagatgaac aaattcaatc tcaagtggta gcggcaggac atcgttcatt aacacatccc
    86341 atagtgagtc gagatcgcgt accttatctt tcgccagacc aaaggtttgg ctaaagtcac
    86401 gataaaaatc ctcctgactc tcaatctcat caaaatcaaa ggtataaata ttcatctgtt
    86461 gccaccgtca cgtttcgcgg cgagttctgc cgttcatcgc taagtatagc catgaaaaaa
    86521 ccgacgcttc aggcgtcggt tttgacttaa ctatcggtca acgcatgttg atacttatgg
    86581 agcatacccg taagccgatg tactggttcc gtcacctgcg gtggcgcttg ccagatgcgt
    86641 agcttttcct ggtagatttc cagttcttcc agcaactggc caaagtaccg acgacgttta
    86701 tcatcgctac gggcagatat cacatggtct gctgtgcggc gcatttgtcg gtgaaacgcc
    86761 gataaatcct cgttaaccgg gatcggcgca tcacgcaggc gctggtgcgc gataatcatc
    86821 gtcagcgcca ggcgaaattt cggcaaatcc cctgggaact tattcatcag caaaaacagc
    86881 tgctgataaa gtgccgggag gtgattctct ttacgacgtg ccacattggt agtcatcgcg
    86941 gaaacagcgg cagaaacaaa ctgattaagc agtacacgtc cagtcctgtc gcgcgattta
    87001 tcccgcacca gcaaaataac ggtgaacgcg agcacacagc cgacgatttg ccctaatgcg
    87061 ctgtcgagaa actgactgaa atggaaagtc atcgggttat ccagcacgat aatgtttatg
    87121 gtgctggcca acgcccccat cgagcccaat ctccgtttct gtacttctat gccgaggaaa
    87181 aatcccagca ctgccaggct aatacacagc agcaacatgc tctgttgggt attagggata
    87241 atcaccaaaa agtagagcag ccctaacggc agcgctgcca gcgtcccgta gataaagtcg
    87301 atcgccacca tgcatggatt cggcaaacgc attgccagtg acgtcacgac cgcaatcatc
    87361 accattgcac cactgccgga agtccagccc gtccacagcc agaaaagcgt gcccagaatg
    87421 caagaaagtg tggttcgcca gaagttaacc attgcatgat gacgttcggc tgactctact
    87481 tttacttccg gttcgccttg caggatctct tcttcggtgg cgttgatttt tgtgttactg
    87541 ataacgccgc gcttgagaag ctgataacgc gttgccgccg cgacccaact ataaatggta
    87601 acaggcgttt cccgttcccc ggtccaggcg atcactctcc gcaggcgttt gagctgcttg
    87661 tggacgtcct gcgcagtttc taccggcgtg tcaaaaaatt cgcggaaagt atcagtgatc
    87721 aattccgggc gcgtattctg aataagataa gtttcgcagg attgggtaat cagcgtcagc
    87781 gatagcgtat tgatcgcttt taaacgtcga ttagcccgcg cccagcggga agattccata
    87841 ttcaggttgc tgcgcatacc ttgtaacgcc gtagtacgtc gcaccagatc gccccaggct
    87901 ttatcgacaa cttcaccatc gccatgcttg atacagagtt gcattaattg atattgcgcg
    87961 accagcaaac tttccagctc tcgatccact tcttgtttga tcgatcgcgg agaaaagagc
    88021 aaatccgcca taattgcaca cacaataccg ataacgatct cgctacaacg ttcgacggca
    88081 aactgcggcg taagcaatgg ttccggctga atggtgatca caatgatcag cgcggtataa
    88141 ccggccagcc cccacgcata cgagttttct attcgtacca gcgaggatat ccaggtacaa
    88201 aaaccggccc agatacagca caccagaatc atcaataatg gtgctcggat catcgcaatg
    88261 atgattacca gtccggcaat acagccaata aatgtgccga tgatgcgcaa aaagccacga
    88321 tagcgaatag cgccagaata cggttcacct cccgcagcaa aggccggacc ggcggcaaca
    88381 atcgccgctg tcagtaccgc ccagcgtggc gtttccagct ggaagtgaaa gccaacaaac
    88441 agcgccagta caatggcggt cgccagtttt accgcaaagc gaatatgttg attagcaatg
    88501 gagaaaatac ccatcgtgat taaccaaact cacgcaggcg atgagccatt ttacggaaga
    88561 acgaatcctg actttcgtcg cgatcttgtt tgccagtgac taccaccgta gcagtggtgc
    88621 ccgcaggcca gatgttctct tgctggttgt cgagacgaat acgtaccgga acacgttgcg
    88681 ccaaacgtac ccattcaagg ttagagtcga tggtcgccat ccctttgtcg tcacgcgtgc
    88741 tgctggcatt ggtgacccct gcggcaacac tatcaacagt ccctttcagc actttgttac
    88801 tgccaagcgg cgtgatctcc gcacgatacc cagggcgcac cccttccagc ttagtttctt
    88861 ccatataggc cagtacatag aaggagttct gtttcaccag cgcaaccgcc gttgatccac
    88921 gggtaataaa ctcaccggta tagacgttga ggttggtcac ccagccatct gctggcgcgc
    88981 ggatcaccgt gcgttcaaga tccagttttg ccagatcgcg ggtcgcctgc gctttcgcta
    89041 actgatgcag aaccgtttgt agtacgttgt tggcctggtc gatctcttcg cgggacatcg
    89101 cctgcacacc gagacggtta cgacgtccgg cctcctggcg tttctcctgt gccagtacct
    89161 gataataagc aacatcggct tgcgcttcct caagcgcctt ttgatagcgc ggctggtcga
    89221 tggtgaacag tacctgtcct tttttcacca gctggttatc atgaacattc acctgggtaa
    89281 tgagtccaga aacgtccggc gcgatcgcaa cgacgtcagc gctaaagcgc gcgtcacgcg
    89341 tccacgggga ttcggtgtaa tagacccagg cattaaaaat tgcgatgaag gccagaatga
    89401 ctaatacgac cgtgatggcc gtacgggaga attttcttat tagtgttttc acttcaacct
    89461 caaacgaaca gtcgcgatat caaataaaac aagcagcaat agagcgcggt gttgaacaac
    89521 gccggatgcc agacaaagtc gtagatacct gttggcacaa gtacccggcg caccagccag
    89581 aaaatcgcca gtgataaaag caattcgaaa aatatcggtg ggaaggacag cccaaacacc
    89641 acgataacgg gaaacagact catgttgacc ttggttgtat agagagagca ggcgttatta
    89701 ttttcagcat ctgtcgccgc agagaagggc atagaaagcc gggcgagagc aacattgctg
    89761 tagattgata tttaatatat taacgtaact gttatgctgt tatctatatt atgtgatcta
    89821 aatcactttt aagtcagagt gaataatgga acgactaaaa cgcatgtcgg tgtttgccaa
    89881 agtagttgaa tttggctctt ttaccgccgc cgccagacag ctacagatga gcgtttcgtc
    89941 catcagtcag acggtgtcaa aactggaaga tgagttgcag gtaaagctgt taaaccgtag
    90001 cacacgcagc attggcctga ccgaagccgg tagaatttac taccagggct gccgtcggat
    90061 gctccatgaa gtgcaggatg ttcatgagca actgtatgcc ttcaataaca cccccatcgg
    90121 gacgctgcgc attggctgtt cttcaactat ggcacaaaat gttctcgccg ggctgacagc
    90181 aaaaatgctg aaagaatacc caggtttgag cgtcaatctg gttaccggaa ttcctgcccc
    90241 cgacctgatt gccgacggtc tggatgtggt gatccgcgtc ggcgcgttgc aagattccag
    90301 cctgttttcc cgccgtctgg gcgcgatgcc aatggtggtg tgcgccgcaa aaagctatct
    90361 cacacaatac ggcataccgg aaaaacccgc cgatttaagt agtcactcat ggcttgaata
    90421 cagcgtgcgg cccgacaatg aatttgaact gatcgcaccg gaagggatct cgactcgcct
    90481 gatcccacaa ggaagatttg tgactaatga tccgatgacg ctggtgcgct ggctgacggc
    90541 gggtgccggg atcgcctacg tgccgctgat gtgggtgatc aacgagatca atcgtgggga
    90601 gctggagatc ctgctgccac gttaccagtc agatccacgc ccggtttatg cgttatatac
    90661 cgaaaaagat aagctgccgc tgaaggtaca ggtcgtgatc aactcgctga cggattattt
    90721 tgttgaggtc ggtaaattgt ttcaggagat gcacgggcgc gggaaagaga agtaattaat
    90781 attgtactgt tacgttgtac aaacctgtgc cgacgggttc ccctaacccc agaggggcga
    90841 ggggaccgtt cgtgcacgga gaaggattaa ttatccttct gaaaatactg aaattattac
    90901 gcagtaccgc caacagtcag gttattaact ttcagcgttg gctggcccac gccaaccggc
    90961 aaactttgcc cttctttacc gcagacaccc acgccgttat ccagtttcag gtcgttgcca
    91021 accatcgaaa tctgctgcat ggtttcgata ccggaaccaa tcaacgttgc gcctttcacc
    91081 ggcttagtta ctttaccgtt ttcaatcaga tacgcttctg aggtggagaa aacgaatttg
    91141 ccggaggtga tatccacctg accgccacca aagttcggtg catagatacc gtactcaacg
    91201 gattcaataa tttcctgcgg ggtcgattta cccggcagca tataggtgtt ggtcatacgc
    91261 ggcatgggca gatgggcgta ggattcacgg cgaccgttgc cagtcggcgt catccccatc
    91321 aaacgcgcgt tgagtttatc ctgcatgtag cctttcagaa tgccgttctc aatcagcacg
    91381 ttgtactggc ctggcgtacc ttcgtcatca atcgccaccg aaccacggcg atcgaccatc
    91441 gtgccatcat caaccacggt acacagttct gaagccacca actccccgac ctgtccacta
    91501 aatactgaag tgccacggcg gttgaagtcg ccttccagac cgtgacccac cgcttcatgc
    91561 aacagcacgc ccggccaacc tgcgccaagt actaccggca tggtgcccgc tggtgcagca
    91621 acggcagaaa gattgaccag cgccatacgc actgcttctt ttgcccacgc atccgcacgg
    91681 acttcgccgt cgagatcggc aaggaagaac tcataaccaa aacgaccgcc gccaccactg
    91741 gcaccgcgtt cgcgtttgcc atcttcttcg accaaaacgc tcacggaaag acgcaccagc
    91801 ggacggacat ccgccgctag tgtgccgtcg gtggccgcaa ccagaattaa ttcatagaca
    91861 ccactgaggc tggcagtcac ttcctgtacg cgcttgtccg cttcgcgggc aaccttatcg
    91921 acgcgacgca ggatatccaa cttctcttca cggctcatgc tttgcagcgg atctaccgag
    91981 gtatataacg ggctatgctc taccgcgccc agcgtctgta ctttgccatc accactatca
    92041 cggacgatgg tgcgcgccgc ttgtgcactc tgttccagcg ccagcaggct gatttggtca
    92101 gcataagcaa atccggtttt ttcaccactg attgcacgca caccaacgcc ctgatcgatg
    92161 ttgtaagaac catctttaat aatacggtct tctaaaaccc aggattcgtg atagctcgac
    92221 tgaaaataga gatcgccata atcaaggcga cgttcggcca gttgaccgag gatcgcgaac
    92281 aagtcctgat gtttcaggcc gttcgccgct agcaattgtt cacttaccag gttcagactc
    92341 atcgtttttg ctactcgtta gttactgcag tagaggattt tttacggctg ccggatgcgg
    92401 cgtgaacgcc ctgtccggcc tacggttatg ttctggctcg ttggcctttg gcaacgatta
    92461 tcatatgaga ttggggcaat tacgcgccct cgtcaaatca ttgcgctttt tctttacgcg
    92521 gttgtcgcaa cacttcgttg atttgcggat cgtccagcgg acccgaaatg tgatagcgca
    92581 aaatggagac tttgctccac agcggtccca gcactttact ggcggcaaac actgccgcgc
    92641 caacaatggg gttaaccgca aaggccgcag ccacacccac cgtcgcagaa atctctggtg
    92701 cgacaaccgc ttccatattc aggtcgcgac gtaccagatt taccgaccct ttcatggcga
    92761 tgtccgcttc caggccatcc accagcgtgt cgtcggtgtg cataacgccg tctttaatcc
    92821 acgcggtgct gcgaatggag tcaaaataga acccttcgcc aaaagtgtct ctgaaatcaa
    92881 aacgcagctt acgcatcagg gcatctacgc tcaataagcg cagcaattgc ccggcatgtc
    92941 cggtattgat ttcggtaatt tcgcctttac ccagttgagt atgaatgatg ccattcaacg
    93001 tcgcctcatc tggctgccag ggtgctttgc gccagtgtaa atcgtaatcc acattaaatg
    93061 acgactggcg tatgggcgtc gtgacaccaa aaaattctgc ggcggcatca attttctgcc
    93121 cgcgcagttt tcctttcagc gaggttcgtt cattccccgg attgttaacc cattcaccat
    93181 cggcagtgag ccgcgcaaaa ccagtatcaa tcagtccatt agtcagcgtt aacgtatcgc
    93241 cagaaatggt gatatcactg tcaatgcgac cgaatttttg cccccagaac cagcactctg
    93301 cgcatcgtat ttgtgcgtct ggccagccac ggaagctaat gcgttccgtt gtcgggaaag
    93361 gtgatgactg tgttgaatca ccacgagttt tcgccacgct tgggttgtaa taaagatatt
    93421 tgatattcgc cagccacggc gcgttattac gcatcgccag cgtggcgttg atttcacgcc
    93481 cttgcgcctc aaccagggtg ccatttgccg tcggttgcga aacaatactc aggttattcc
    93541 attgctgatt tcccagcgac aacataggcg tacgtaacgt tatgtgttgt gggaaactcg
    93601 ctgcaccacc gacactctcc gccgcgcctt tctggaacag ggccagccac tcggcaccat
    93661 tcatcggcgg catattgagt tcaacgccac tttgttccgg caacggcggg agcgttttac
    93721 tgtctgccgc ccaaatagca cgatcgagcg tcagcttttg accgagcaac cagcggctat
    93781 tgaaatagtt atccgcacca gcctgtccgg ttaattcaaa gctgttgaga ttgccatcaa
    93841 ccttaacgtt taccgatagt ggttcacccg caggtttggc taacggtgaa ggtaagtgac
    93901 tgctcacatt cttcaaatcg ccgttcagct ctacgttata cgtcgcgcca gcatgataag
    93961 gcagctcaat gcccacttta ccatcccacg ccacgctgcc acttaatgct tcgttcaccg
    94021 ctgcaggcag aacgccggtt ttcgctggtt gccagttacc gttgagattc accgcgacct
    94081 ggtaggcttt tgccccttct ttggtggaaa aatccacgtt caacggctga ttaaaccagc
    94141 ttgctgtcag tggttcactt tgcagatcgc cattgataaa gctgaattta ccgctcaaat
    94201 ttttcagggt gctgtcgagt ggtttgataa acagactgtt attacgcagc gtcacttcac
    94261 ctttcgcggt taccaactcg ccgttcagcg ggatatcaag atgtaagcga gcattcacat
    94321 cgccgtcgag ctggagttct tgcagggtcg cacccaggga atctttcagc ggtgtctcat
    94381 caaagtaagg gccaacggct ttacccggac ctttaatgtc agcgtcaatc agcagttttt
    94441 cttttgagta gtcagggatc actgcggtga gattactcgc gcgcacgccg cccagattaa
    94501 cgccatcggt tttcatccat aaaccgtcgt taataaagtc cagttcaata tcaaggttag
    94561 ttaatgcagg ccagtccggc tggaaggcaa acttcgcgtt gcgcagcggc accagcactt
    94621 caaactgacc ttcgttgtgt ttatagggga agagttgcgg attgccacca taaaccagcg
    94681 tcgcgttatc cgcttcaccg ccctgaatcg cgccacttaa gtaatcaacc aggtctttac
    94741 ccatcaagtt ttccgggaaa tagcgccagg cttgtgaacc atcatcggta ctgatgccag
    94801 ccagaatacc cagccagggt tcatcgttag caggttgcag gtaacgaaaa ccgccgcgcg
    94861 catggacggc tttggcttta acgtcaatat tacgcccatc cagctggaaa cctttgtcat
    94921 tgttcagcca gcttatcgtt gcctggccgt cggcgatttc cagtggcgca cggaataccg
    94981 tttcgtaagg catctttgcc tgcttcatcg acgcagtaag caaaccattt tctacgctgc
    95041 cggaaagcgt cccggagaag tgttccgcac ccggtaataa tttccattgc ttccaggcca
    95101 gatcgctcca cgatgcctga aaacgggtct tgtctgccgc ctgaagcggg atatccagcg
    95161 ccagagtgtt aatcttgccg ctcggttgtg tggagcgcca aacatcaccc agtgcaggtg
    95221 aaagtttcgc gaccagcggg cgtacgccct ccagccctgc cagctccaga ttactggcac
    95281 gaatccggag ttcgtcactg cgtttattgt ctttgccgcc aacgtcctgt tccggtatcc
    95341 aggccagcgt caatgctccg ctcggccagg gtttgccgtc catcgtgatc cgtgtatcgg
    95401 gaatagagaa ctgccatccc ggattttcac gcgtaatatg cgcagtcaga ttatccaccg
    95461 acagcgtatg cgtttccttc tcgcccaacc agctggcacc gccctgtttc agccagacgt
    95521 caccgccggt tacatcgcct ttgtcgatcg tcatccagcc ttcaagggag aactgtgccg
    95581 tttccagcgc aatattgtcc tgcatccatt taccgagcca cggcttcagg tcgatgtcat
    95641 ccgcctggag ccagacgcga ccattgctta acaacccctc atcatcgcgc aaatccatac
    95701 gcacctgcat cacgccgtgc tgtccggtaa ggctggagag gcttaccagg ccttccgcac
    95761 ggtgtcgacg tggatcgttc agccaggtga gctgtgggat cgccagctcg gcgcgctgac
    95821 cggatggcgt caggaaactg acttcactgt cgcggagatc gaaatggtca aattgacgaa
    95881 gaaacagatc gctgatgtga ctggcttcca ggctatcatt accaccgccg ctggtgatag
    95941 aagtgttggt gcgaaagcgc agctgccaga aagtgaggtc gcgaaactgc cagcgcatat
    96001 gtaacaggct ctgccagaca tccagcgcca gggtaacgcg tttaaccgaa aattcgccgc
    96061 catcttttag ttctgcacgg atgtcgtgcg cttcaagcgt cggaccaaaa ttctgccagc
    96121 tggctgagag ctgactggct tctaccggca tgccagtcgc ggattctatt ttgttgagga
    96181 tttccggacg ccaggcgtca agatgcggta aagcaatacg caggccgcta accagcaggg
    96241 cagcgatcac aacgagcgcg gctccagtaa gcagtaaaat ccccggcaat cgcctcaccc
    96301 gtcactcctt gtctgctaaa aatgtgactc aaaaaccctt tgccggatgg cggcccagca
    96361 tctgtttaca tcattacgac gtcaaactgc tcctggttat agagcggttc aatttgtact
    96421 ttaacctgtt tgccaacgaa aatttccact tccgccagcg agtgtgactc ttcgcctttc
    96481 aaggcttcgg ctactgccgg agaagcatag accaggaaac ggtcggagtc gtaagcatgg
    96541 tggacacgaa caatctcgcg catgatttca tagcataccg tttccacggt tttcaccgtt
    96601 ccgcgaccgt ggcaggttgg gcattcgtta cacagtacgt gctcaatgct ttcgcgggtg
    96661 cgtttacgcg tcatctccac cagccccagc gccgaaaaac cattaacgct ggttttcacc
    96721 cgatctttgc tcaacgcctg ctccagcgag tgcagcacgc ggcgacggtg atcttcatta
    96781 ttcatatcga tgaaatcaat aatgataatc ccgcccagat tgcgcaaccg taactggcga
    96841 gcgatagcct gcgtcgcttc aatattggta ttgaaaatgg tgtcgtccag attgcgatga
    96901 ccgacaaacg ccccggtatt gatgtccacg gtggtcatcg cttcggtctg gtcgataatc
    96961 agataaccac cggatttcag ttctactttg cgttccagcg ctcgctggat ttcgttttcg
    97021 acatcaaaga gatcgaaaat cggctggcgt ccggtgtaat gctccagctt gctggtcatc
    97081 tcgggaatgt actctgaggt aaactccagc aatgcttcgt aagtcaggcg tgagtcaacg
    97141 cgaatgcggt cgagttcggc gtcggcgaaa tcacgcagaa cacgctgcgc cagcgccagt
    97201 tcgccgtaca gctgataacg ggtctgcggg cgttttttac gctccataac tttggtccag
    97261 acgcgtttca gataagcagc atcggaggcc agttcggcct cgccaacccc ttccgctgca
    97321 gtacggatga taaacccgcc ctgctcgtcg caatactctg cgaccacttt tttcaggcgt
    97381 tcacgttctg attcgctttc aattcgttgg gataccccaa cgtgagaagc ccctggcata
    97441 aacaccagat agcgagaagg cagcgtgata tcggtggtca ggcgcgcacc cttagtgcca
    97501 agcggatctt tcaccacctg caccatcaga tcttgcccct gacggaccag ttccgagatg
    97561 tcgcgcaccg tgaattgctt ttgttcttca cccgccacac attcggtgtg cggcatgatg
    97621 tcagatgcat gaagaaacgc ggctttatcc agcccaatat ctacaaaagc cgcctgcata
    97681 cccggaagta cacgacttac acgacccttg tagatattgc ctactattcc gcgtcgcgcc
    97741 tcacgttcaa tatgaatttc ctgcagaata ccgccatcaa tatacgccac tcgagtttcc
    97801 gaaggcgtta cgtttaccaa caattcagcc gtcatgttta tcccttttct cacgcagtgc
    97861 gttaaaatta cttaataatt catacgtttc aaccagcggt aagccgacta cggcgtgaaa
    97921 gctgccattt atcttcctga caaaacagcc acccagcccc tgaataccgt atgcacctgc
    97981 tttatctaac ggttcgccgc tggcgacata gcccgcgatg tcttcgtctg ttaacgttct
    98041 gaaagtcaca tcggtgacca ccaggcaatc gagaatgtgc tggctgtcgg ccaacgccac
    98101 tgccgtcatc acctgatggg tctgacccga taatttgcgc aacatctgcg ccgcatgctc
    98161 tgcgtcgcgc ggtttctcga gcacttctcc attcaggata acgatagtat ccgcacccag
    98221 caccgggaga tcctgcgccg tttgcgcgac acccgcctgt gctttctcgc gcgccagacg
    98281 cacaacatac tgctgcgcgc tctcctgcgg ctgacgctgc tcctcaatgc ccgtaacaat
    98341 acgttcaaag gtcacgccaa gttgcgcaag taactcctga cgacgcggag aaccggaagc
    98401 taaatacaga gaagtcatag aaacctttat tgcactgcaa actgctgacg gactttacgc
    98461 atcagcaaga aaatccacgg ccagagaacg ccattgacta cactactcca gaacacttcc
    98521 ggtctgaaag agacgttaat cactaaaaac tctgcccaga aaacaataat atccaccacc
    98581 agcgaaagca acatgacgac cagcgcctgc tgccataatg cgaggttgcg gaaaagctgg
    98641 tatttcagcg ccaccaggta agcaatgatg ctcatcgcca atacgcgtac gccaagcgtc
    98701 gagccgctga tcagatccag tatggcaccc atcacaaaac ctgtgcccac atttacgcga
    98761 tggggcaagg ccaggatcca atacaacaag atgagtaaca cccagtttgg ccggaaaaca
    98821 atcaggttgt ccggccatgg catgatttgc agcaacagcg caatgaggaa agagagccag
    98881 attacccagc gtccctggct acgatagctc gccactattg tcctcccggc gcacgcgcag
    98941 gcggttgagc accactttgc ggcggcgtag ccctttgtgg agagcgatta gcagcgggct
    99001 gtgtgggcgc agcaggcgca gtaactgcat ttcctgtcgc cggttgctgc ggagtcggct
    99061 gagtgatccc cgttgccggt tcaggtaact ttggccccat cgcgtctggc gaaggcaata
    99121 cctgcggcat catctgcatc agacgttcat tagcaacacg atgcacctct tccggcgtca
    99181 tcgggttagc gccgttacga tccgcgcccc acagcagcag cagataacgc aaacgttgca
    99241 gtcctgcagt cggacgcgcc tgaatcacag tataagcgcg ctgggtatcg agttttacgg
    99301 aagagacaac cgcgaccgga tagccttccg ggaaacgacc gcccagaccg gaagtcacca
    99361 gcacatcacc aacacgaata tccgtattcg ctggcagatg ctcaagctgc aaatcatccg
    99421 tacaaccgtt accggctgca attacgcgga tatcgttgcg cagcacctgg attggcagcg
    99481 cgtgggtcgc atcacaaatc agcagcacgc ggctggtcag tttagcgacg gccaccacct
    99541 gaccaacaac acctttgtcg ctgataaccg gctggccttc ataaacgcca ttaacgctac
    99601 ctttatcgat aacaacttga tcgctataag gatcgttaac cgtggagata acctgagtca
    99661 ccattttctg ctcatcctga cgcagcgggg aacccagcag ctcgcgcaga cgcgcgttct
    99721 cctgtttgta ttgtccaagc atcagcagtt cactgttttt cagcaacagt tcctgacgta
    99781 acgcccggtt ttcaagttct agttggtcac gcgaggccag cgtctgcgat acgccatcca
    99841 gcaattcacg aggagcattg gaaacaaagt agaaaggact gacggcggta tccatataag
    99901 tacggatttg actgaacgtc cccaggcggc tgtcggcaat aataatgccg agcgccacca
    99961 gcaccgccag aataaggcga atctgtagcg acgggccacg gctaaaaatt ggcttcataa
   100021 gttatgcgta ttctcgtatc agaccaggca gggtaaacag acacttcccc tgcctgcatc
   100081 cgattactct tcgctgaaca ggtcgccgcc gtgcatgtcg atcatttcca gcgctttgcc
   100141 gccaccgcgc gccacacagg tcagcgggtc ttcagcaaca acgactggaa tgccggtttc
   100201 ttccattaac aaacggtcaa ggttacgcag cagtgcgcca ccaccggtga gcaccatgcc
   100261 gcgctcggag atgtcggaag ccagttccgg cgggcactgt tccagtgcaa ccattaccgc
   100321 gctcacaata ccggtcagcg gttcctgcag tgcttcgagg atttcgttgg agttcagggt
   100381 aaaaccgcgt ggaacacctt ctgcaaggtt acggccacga acttcgattt cacggacttc
   100441 atcgcccggg taagccgaac cgatttcgtg cttgatacgt tctgcggtgg cttcaccgat
   100501 cagagaaccg taattacgac gtacatagtt gatgatagct tcgtcgaaac ggtcaccacc
   100561 aatgcgcacg gaagaggagt aaaccacgcc gttcaaggag ataacagcaa cttcagtagt
   100621 accaccgccg atatcaacca ccatagaacc ggtcgcttca gaaaccggca ggccagcacc
   100681 aattgcggca gccatcggtt cttcaatcag gaagacttca cgggcaccag cgccctgcgc
   100741 ggattcacga attgcgcggc gttcaacctg ggtcgcgcca accggcacac aaaccagaac
   100801 gcgcgggctt ggacgcataa agctgttgct gtgcacttgt ttgatgaagt gctggagcat
   100861 tttttcagtc acgaagaagt cggcgataac gccgtctttc attgggcgaa tggcagcaat
   100921 attgcccggc gtacggccca gcatctgctt cgcgtcatga cctactgcag ctacgctttt
   100981 cggtgaaccg gcacgatcct gacgaatggc caccacggaa ggctcattca atacgatgcc
   101041 ttgtcctttt acataaatga gggtattcgc agtacccagg tcaatggaca agtcattgga
   101101 aaacatgcca cgaaattttt tcaacatact aagggataat cctgaaagct ggggcggaaa
   101161 agaaaatccg cttactttac caaccacacg cagcagcgac aaggcgcaaa aatcatctgc
   101221 tacggtgaaa attagtgcag ttcgtttcct ttgttacaaa tctctgcctg agtccagaaa
   101281 ggcttaatgc atcagcagca ttcctcgcct gtttgcaacc gcgtaaggtc attcatctgc
   101341 atatgtgctg caacaatctg gcgagcagac aagcacactc ccatgagacg cagcgcgcgt
   101401 tattctacgt gaaaacggat taaacggcag gttaaaccga gtatctttgt gaatattttt
   101461 tcacgttagt atcaagtggc tgtgaggacg cgaaaaaatc cccttgccca cctgtaacac
   101521 cgcgctgaat cagggtctgc cactcgcttc gcgaacgcac gccggtggcg taaacctggg
   101581 tgctggtccc ggagcaggct tccaccaggc tttgaaccag cagctggttc tccgttcgct
   101641 tctcaatgtt tctgaccagc cccggatgga gcttgagtaa ctcaacatta agttctttga
   101701 tccaactggt gcttaccagc gtcaaaccag cctggttgac ggctacccgt acccctaaag
   101761 cattcactaa acgaataaca ggttgtaagc gactgatatg ttgaccaaca tcggcctctg
   101821 caagttcaat aattatgcgt cttcgttgcg atttttcaca ttgcattaac gtatcgcgca
   101881 gccaacgctg aaaacgcggg cgaatcagcg actcaacggt aacctgaatc gccagatttt
   101941 cctctggcca gtaacgcaat agtggaataa gacggctgat tagcaagcgg tcatactctt
   102001 ccgataaacc aaactgcaag accatcggca tatactctgc cgagctaacc tcttcattac
   102061 catcgaagat gcggcacatg agttcacgat gatgaacccg gccttcgcga gtaaccgccg
   102121 gtttttgata aaggcgcggg ccgccgcgac tgagcatttg ctcgataagc gtacgccagc
   102181 gaacattacc gcgtcctttt tcaggcaacg agtcatcgta aatagcccag ctattgccgc
   102241 cctgcaatcc cgcattacgc gtggcagact ctgcatgttc cattacctgc tcggtatcct
   102301 gaccactacg ccaggcacag ataccaatgt ggatcatatc gtcgcgatcg agcattttat
   102361 tgttcggcaa ggtatcgacg gctttgatta actgactggc gatgctctct gcctctttta
   102421 gcgtccggtg cggtaacagc gcagcaaaat cactgcgatg gtaacgcgcc agcagtgcgc
   102481 cagggtagcg catcataaat gtcgatagca gattcgtcag agagaagaat tgttcttcaa
   102541 ccaggctgtg cccccaggta tcactcaaca tattgaaatc cggcagacga atcatcatca
   102601 cgatcccgtg ggtacctact ttctcttgat cttccagtaa cgttgctaac tgattatcga
   102661 aaaagagtcg gttattgagg ccggttttca tgtcctgggc ggcataagag cggatcagcg
   102721 tatcaagacg gctgtgttgt tcgcgtgcgt tctgaatttc acaaagcagc gtatccagcg
   102781 cactgctggt tctgggcggc cattcataga tggttcccaa cacattagag ccacgctcac
   102841 cgtttaagat acgagtagcc cgggtttcca gcaattcttg cccggcaagt tgccgttgta
   102901 accagcgtac cgccaggaag agcataagaa tgataaagcc aatcgccccc gtgagcggcg
   102961 cggtggtcat taaagaatgg aaatagttgc ccatcggatc ctgataaacc agacgcagcg
   103021 acatccccgg atgctttatc aacggaacgc tcagttcacg aaacagatcg ttggtgccaa
   103081 ccggacgata actaccattt ctggccaggg tataaacctg cttatcacca tggagcaggt
   103141 ctacacgaac gatatctgcc gacatcatta attcggtaat ttgtggcctt aatgtgctga
   103201 agtcattcga cagtaggtgg gtatcaatcg ccgtcgccac cgcctgaaca cgatgactaa
   103261 acttatactg aatggcgttg tagaaactta gcgaacagcc cagcaaagtc acaaaaattg
   103321 ttaacccggt gagcagcgtg acaaaggccg aaaatttcgt cgttaatctc atccttgtgt
   103381 taactccgat agtgaggaag cgggcatact agcaaatcag atttatctcg caatttattg
   103441 cgcttcatcg gctttgcttt tccattagcg agtatagtct tcagaaatta ttttccaatc
   103501 catcatgcac atgaggacca cttatgcagg cgttactttt agaacagcaa gacggcaaaa
   103561 ctctcgcatc agtacagact ctggacgaaa gtcgcctgcc ggaaggcgat gtcacggttg
   103621 atgttcactg gtcgagcctg aactacaaag atgcgctggc gattaccggt aagggaaaaa
   103681 tcatccgtaa ttttccgatg attcctggga tcgattttgc cggaactgta cgcaccagcg
   103741 aagatccgcg ttttcatgcc ggtcaggagg tgttactcac tggctggggc gttggtgaaa
   103801 accactgggg tgggctggcg gaacaggcgc gagtgaaagg tgactggctg gttgccatgc
   103861 cgcaagggct ggacgcgcgt aaagcaatga ttatcggtac tgccggtttt accgccatgc
   103921 tgtgtgtgat ggcgctggaa gatgccggtg ttcgcccgca ggatggggag attgtcgtga
   103981 cgggtgccag tggtggcgtc ggcagtaccg ccgtggcgct gctgcataag ttgggttatc
   104041 aggtcgttgc tgtttccggt cgcgaaagta cccatgaata tctgaaaagt ttaggtgcca
   104101 gccgtattct tcctcgcgat gagtttgccg aatcccgtcc tctggaaaaa caagtctggg
   104161 ctggggcaat tgacaccgtt ggcgacaaag tgctggcaaa agtgctggcg caaatgaatt
   104221 acggcggctg cgtggcggcc tgtggtctgg cgggtggttt tactctgcca accacggtca
   104281 tgccatttat tctgcgtaat gtccgtttgc aaggggtgga ttcagtaatg acgccaccag
   104341 cacgccgtgc acaagcctgg cagcgactgg tcaccgattt accggaatca ttctataccc
   104401 aggcggcaaa agagatatct ctggcagagg caccgaagtt tgccgaggcc atcattaata
   104461 accagatcca gggtcgcacg ctggtgaagg ttaactaacc atttagcagg gaataataag
   104521 agagggaact cattttgaaa tcattattca atcagttaac gggaaaagcg gttagccgga
   104581 cagctttcgt cgaacatctt ggttatgaag ttgtacaaca tcatccaaac tggaaagtca
   104641 tgatttcgac tgaccataaa ttgatgcgca ttgatgctcc accaaacagc cattattgat
   104701 acgcctccgt cgcctgttag gtttatggtg ctttgccttg gcgacgctac gcttaatctg
   104761 ttactgattt ctggtaccat ggggggaata atctgatttt atttgattac aaattaacca
   104821 cttaaaccta ttgaatgcgg agcattgtca gtcggttaat tttgcgtgct ttagcattca
   104881 catctttccg gacgatgcag tgaaaattgg gtaatcccca gcaaccgctg cgtaatgtcg
   104941 tctatcttgt cgcgatcctg gcatccctac attatttgtg gtctgtgaag attatctcat
   105001 tgcagccccc atcttcgcag ggctggcttt acagcgtttc accttacgtt ataagaagtt
   105061 ccgtcgatga tggcgctaat ttcgtgaatt gtgcggcttg ttgcaaatta cacggtgttg
   105121 aaggttattt acatgttagc tgttgattat cttccctgat aagaccagta tttagctgcc
   105181 aattgctacg aaatcgttat aatgtgcgac ctcgtcctcc ctgacgcagt ttttgcactg
   105241 cggaaaaggt gacattggcg caacgaaggt atattttgtt ttttgccgga ggatagcagc
   105301 agatcgctgc acaatgtccg tcaagtctaa cattgacact ctggggcaaa atagaccggc
   105361 gtcccggcct gctggaattt atcgctatgc atacagctgt cggggcatac gctttacaga
   105421 cggcggtgaa acgcctgtca caatcacact aaacaaagag tacggaaccc actcatggat
   105481 attcgtaaga ttaaaaaact gatcgagctg gttgaagaat caggcatctc cgaactggaa
   105541 atttctgaag gtgaagagtc agtacgcatt agccgtgcag ctcctgccgc aagtttccct
   105601 gtgatgcaac aagcttacgc tgcacctatg atgcagcagc cagctcaatc taacgcagcc
   105661 gctccggcga ccgttccttc catggaagcg ccagcagcag cggaaatcag tggtcacatc
   105721 gtacgttccc cgatggttgg tactttctac cgcaccccaa gcccggacgc aaaagcgttc
   105781 atcgaagtgg gtcagaaagt aaacgtgggc gataccctgt gcatcgttga agccatgaaa
   105841 atgatgaacc agatcgaagc ggacaaatcc ggtaccgtga aagcaattct ggtcgaaagt
   105901 ggacaaccgg tagaatttga cgagccgctg gtcgtcatcg agtaacgagg cgaacatgct
   105961 ggataaaatt gttattgcca accgcggcga gattgcattg cgtattcttc gtgcctgtaa
   106021 agaactgggc atcaagactg tcgctgtgca ctccagtgcg gatcgcgatc taaaacacgt
   106081 attactggca gatgaaacgg tctgtatcgg ccctgctccg tcagtaaaaa gttatctgaa
   106141 catcccggca atcatcagcg ccgctgaaat caccggcgca gtagcaatcc atccgggtta
   106201 cggcttcctc tccgagaacg ccaattttgc cgagcaggtt gaacgctccg gctttatctt
   106261 catcggcccg aaagcagaaa ccattcgcct gatgggcgac aaagtatccg caatcgcggc
   106321 gatgaaaaaa gcaggcgttc cttgcgtacc gggttctgac ggcccgctgg gcgacgatat
   106381 ggataaaaac cgcgccattg ctaaacgcat tggttatccg gtgattatca aagcctccgg
   106441 cggcggcggt ggtcgcggta tgcgcgtagt gcgcagcgac gctgaactgg cacaatccat
   106501 ctccatgacc cgtgcggaag cgaaagcagc tttcagcaac gatatggttt acatggagaa
   106561 atacctggaa aatcctcgcc acgtcgagat tcaggtactg gctgacggtc agggcaacgc
   106621 tatctatctg gcggaacgtg actgctccat gcagcgccgc caccagaaag tggtcgaaga
   106681 agcaccggct ccgggcatta ccccggaact gcgtcgctac atcggcgaac gttgcgctaa
   106741 agcgtgtgtt gatatcggct atcgcggtgc aggtactttc gagttcctgt tcgaaaacgg
   106801 cgagttctat ttcatcgaaa tgaacacccg tattcaggta gaacacccgg ttactgaaat
   106861 gatcaccggc gttgacctga tcaaagaaca gctgcgtatc gctgccggtc aaccgctgtc
   106921 gatcaagcaa gaagaagttc acgttcgcgg ccatgcggtg gaatgccgta tcaacgccga
   106981 agatccgaac accttcctgc caagtccggg caaaatcacc cgtttccacg cacctggtgg
   107041 ttttggcgta cgttgggaat ctcatatcta cgcgggctac accgtaccgc cgtactatga
   107101 ctcaatgatc ggtaagctga tttgctacgg tgaaaaccgt gacgtggcga ttgctcgcat
   107161 gaagaatgcg ctgcaggagc tgatcatcga cggtatcaaa accaacgttg atctgcagat
   107221 ccgcatcatg aatgacgaga acttccagca tggtggcact aacatccact atctggagaa
   107281 aaaactcggt cttcaggaaa aataatttgt gacgcgcggt gttttcatcg cgcttagcaa
   107341 agaacgtaat caacgccggg cctgttaatg ctacccggcg tttttattgc cgttaactaa
   107401 cggtagcctg caatttccgc tttcaacgcg ctaatttcag catcgccatc aacaattccc
   107461 agctcaaagc agtatttctt tgtttcccct ggctcaataa agggcagacg cccctgcgct
   107521 cgttctttat cacgaccgtc aggaaaacta ttactgacct ccagtcccat aacatattca
   107581 cccgcgccta aatttttcca ctggataaag ttgggtagct gcatggtgtc gaatttctcg
   107641 taaattcccc accccagttc ggcgttcacc agcgcaactt tactcatccc ctgactgtcg
   107701 ctattcaacg tgagatagta aacctgctcg tcaaaaccgg gctgtggggt acaaatctcc
   107761 agatgactgg ttaacccttc cgccgcatgc ggagttcgtg gcgttatgct tttagccgac
   107821 agcaagattt ctgcctgtgg cgataataaa ggccagccgt aattaaagtg ataaagaatt
   107881 tgcagcggct ggcgggtgaa accatgatta gtcaccttgt cttcaatgcg cattacattt
   107941 tcgccataac ggcagcgaat ggtcctttcc agcgtcagat tttcaccgaa caatcgtgtt
   108001 tcccgtactt tgccattgat gacaaattca atgctgtcgt cggtccgttc tgtgcgatac
   108061 cccacctctt ctgctggagt cgcagccaaa cgcccgtgta accccagcgt ttctccttca
   108121 tcctcacagg gtgtgccaat gtagctaagt ccgcaggtag tcagtaaccc cgcgtaaaag
   108181 ctacgtaaaa acccgttgcc ctgagactca aaaaaagcgg gggcgacaac accgttgggg
   108241 gtgataaaag agagcgcagt gtccttgtag ctggcccatg caatatccag cgcgcgatct
   108301 tttaccaccg taaatgccag tcccgcacca gtacggacat caaacgcttc tgtacccttt
   108361 gctctgccac tacgtaactc atagtgttta attccggcaa cctgcgagag atcgccaatc
   108421 cacgaccaaa tttcgttttt cattgtgctg cttcccgaat ggacatgtca tggcgctgga
   108481 tgaacgtttc gatttccata aggcgagacg ttccctccat tgggccacgt cgggttaccg
   108541 ccagtgcacc gcaggcgttg gcatattgta atgcccgatg ggcgtcaaac cctagctggc
   108601 ggcaggcaat ccacgcgcca ccaaagcagt ctcctgcacc ggtcgggtca acttcttcca
   108661 ccggatagct ctcaacatga aactgctcat tcgcggagta gtaactggct ccttgattgc
   108721 cgcgcttcac aatcacctct ttaacacctt cttcaagaaa accagcgatt gcacgctccg
   108781 gcgtcgagtg tggcgacaag agcaacactt ccccctcact gggcatatag atatccgtta
   108841 actcaagaac aaagtggagg gcatcgcgca tttctggaat atcaagcatt tctttacgaa
   108901 tattcgggtc gaaagaaata acgccaccat ttgctttaac gatggttact gcttttttta
   108961 ctgcgtctac catatggaat gaaaacaggg atgaacccat aatatgaaaa tgggtacaat
   109021 ccttcagaat attttcatca acatgctgtg ccgataattt accgcaagcg gcattcttga
   109081 tattaaaaat aaaatcgcga tcgccagagt tatgataagt aacaaaggca ctaccggtgg
   109141 cttccagagg taagactgaa atccccctga tgtccacacc gtcagcagcc agacgatgta
   109201 tattaatatc accaaagcca tcattcccca cgcagctaat gatgccgcac ggaacgccaa
   109261 gacgtgtcac ctggtcgata aaaatagcgg gcgcaccact gggataaggg ccgttccaga
   109321 tacctggttg atcaaatggc tggccgattt tactcgccat aatttcaacc agaatttctc
   109381 caattgtgaa gaccttactc atatgattaa ttccttgaaa tgattagctg cgttttcttt
   109441 tatttcttac gtcaaaataa acggctacaa gaatgactaa tcctttaacc accatctggt
   109501 aaaaataagg aacattcatt aagttcatgc cattattaat aacgccgatg gttaatgcgc
   109561 cgatcaccgt ccccaccacg gttccatagc cgccgttcag gctggtgcct cccagtacag
   109621 ctgcggcaat agcatccagt tcgtagctca acgcggaatt cggttgcccg gaatagaggc
   109681 gtgaggcaag gatcatcccg ctgatacctg ccagcacggc tgtaatcata aagactttaa
   109741 ttttcaagcg ggtgacattt atccctgaat aaattgccgc ttctttatta ccaccagtga
   109801 tataaatttt tcgtccaaag gttgttttag taagaatgaa gtggtttatc agcaataaga
   109861 tgattaatat ccatatagga ataggtatat agagaaattc tccattcccc agcgccagaa
   109921 aggcatcatc tttaatcatg atgggcatac catcggtcac tatataagcg ataccacgga
   109981 agatccccat agtagcaacg gtgacaataa acgaagggat ccccgctata gccgtcaagc
   110041 caccatttat catccccaga gccagtgctg caataatgga taatgggacc aataaaacaa
   110101 atggaattcc ccatgaagcc ccaagcgcag caaatgtgcc aatcaatgca atggttgaac
   110161 cgaccgataa atcaatatcg ccaagcaaaa gcacataagt cattccatag gcggtaattg
   110221 caattatcga aacctgaaga ataatattag taatgttatt ggcggtaaaa aaactgtcat
   110281 taataaaact gaacagaatg attaacacca ccattccact gaggatacca ccatattgcc
   110341 tgaagacttt tttaaaatta tttgcaatgg gaattcccga aggtttcgga ctggcaagag
   110401 ttttctctgt catttaacgt actcctgttg cagcggtcat tacctgtgtc tgcgttaaat
   110461 tttcacatga ataaatatcg gcaatagttt tattacgcat taccaccaga cgatcgctaa
   110521 gtgccatgac ttctggcagt tcagaggaca ccagaataat tgctgtccct gccgcagcca
   110581 gttgacgtat cacgttatag atttcaaatt ttgcgccgac atccactccg cgcgttggct
   110641 catcgagcaa taaaatacgc ggagttttct ccagccactt cgataacacg attttctgtt
   110701 gattaccgcc gctcagtgct cccactgcct gatgcacaga actcacttta gtgttcattc
   110761 tggcaattga ttgcagcgtc ttcgcctctt cttgtcggtg attgatgagt cccatccgcg
   110821 aagcgttata ttgaaacgcc acatggtgcg tgttcatatt gacgtcgtgc atcagcacta
   110881 acccttcttc cttgcggttt tcggtcacaa aagcaatgcc atgatcaata gcctgtttgg
   110941 gattagcgat cctgacttcc ttaccatcaa tcaacacagt cccgtgatac gaaacgaggc
   111001 caaatagcgc cttcatcaca tcggaacgcc cggcccccac caggccaaaa aatccgagga
   111061 cttcccccgg acgcacagcg aaactgacct ctttaaaaag cgaaggatgg gaaagatttt
   111121 ttacttccag ttttgcagga acattttcat ccggtttttc cccaagacgt gctggccaaa
   111181 tatttttcat ttcccgacca accatttggg caatcaagtg ctggatatca ctgtttttct
   111241 tttcatgcgt agcgatatac tcaccgtcgc ggatcaccga aatatcatct gaaatccgca
   111301 taatttcatc catgcgatga gagatataaa taaccgcaca gcctcgctgc tttaattttt
   111361 caataatgtt aaacaggatc tccgcttcat tatcacttag cgaagatgtc ggttcatcca
   111421 taatcacgac atccgctgca tagcttaagg ctttagcaat ttctaccagt tgcctatgtg
   111481 ccatcgacat attgccgacc atttccagag ggtctagtgg gatgcccagt tccgcgagga
   111541 ggttctggca atctgcgagc atttttttat cgttaatgat gccaaaacgt cgcggttcat
   111601 tattggcata aatattttcc gccacggtca tattattact caggctcagt tcctgaaaaa
   111661 taatcgcaat ccctaactgg cgagaatgct gcggattatt aatggtgatg gtttcaccac
   111721 gtagtgttat cgttccctgg gttggcggat aaacaccggc cagtattttc attaatgtcg
   111781 atttaccggc accattctcg cctaataaag tatgcacccg ccctttacgt aaagagagtg
   111841 taatatcttt taatgcctga acatgaccaa atgtcttcgc aatatgcgaa acatcaagaa
   111901 taacatccgt cataatgacc tcggtgtcac aacaaaacgc caataacgtt ttgttgtgag
   111961 gtgaagatat ttactattta cctgtataaa cgccgggttg taccgggatc attttttcta
   112021 ccggtttacc attgaggtga tcaacagcgg catcaatggc tttagcaccc atttgatctg
   112081 gatattgccg aatcacggcc gcaaacgttt tttcactgtc aacggcatca cgcgcttctt
   112141 tcatgccgtc aaaaccaatg attttgatgt tgtcgttatg cgcagatttt gctgcgataa
   112201 ccgctgccag cgccgcatca tcaccaaagc caaacagacc attcaggtta gcatttgcct
   112261 gcataatgtt ttgcgcagtc gttaacgctt cagctcgcgt aataccaggc tgttcagcaa
   112321 caattttaat atccggtgta tcgcttaacc ctttcttaaa accatcaacg cgcgcaacca
   112381 ccgattgtag tgccggataa gaaataatgg ctacagtacc tttaccactc aaaagtttac
   112441 ccattaactt gcctgcttca acgcctcccg catagttatc ggtcgcaaca tgcgatacga
   112501 ccggaacgcc ttccgcagcg acgtcaacgg tgataacggg aataccggcc ttatcggctt
   112561 taattatcgc tgcctggacg cctttcgaat cgaccggaga gataataatg gcatccactt
   112621 ttttagtaat aaaatcctca acatcagaaa gttgtttgtt taaatcctga ttggcaatag
   112681 agacacttaa attgacattg tctttttttg cctcttcttt catggcattc gcaagatcaa
   112741 tatagaaagg atgttgttgt gttaataatg acgcaccaat tttatactct tttgccaacg
   112801 aactttgcga gaacaatgcc aggctcacca acatagctga accaagtaat ttaatatttt
   112861 tcataaacaa tcctcattat ttttgtaggg tacaacgtct taaatttttc cttcactacc
   112921 acaaacgcca ataatacgag ttacgacttc tttcatggcg gcctttgcgg gctgcatata
   112981 gtgacgtgga tcatttgccg caggattgtt gtagaaatat ccttttaacg catcggcaaa
   113041 cgcaattttc aattctgtcg cgacattcac tttacaaata ccgcgacgaa tacagtgctg
   113101 aacgtcctgt tccgataagc ccgaagcacc atgcaacacc agcggaatct ccactcgctg
   113161 acgaatctgt tccagccgca caaaatccag tcgtggttca tgcgcatata aaccatgtgc
   113221 tgtgccgatt gctaccgcca gggagtcaat ttgggtacgt tgaacaaact ctgtagctgc
   113281 ggctggcgag gtataagcgg aatctttttc atccacccgc agatcgtcct cctggccgcc
   113341 aagacgccct agctcggcct caacgctaac atcaaagcga tggcaaaagg tggtaacttc
   113401 ttgcactcgc gcaatgttgt cttcaaatgg caaatgggag ccatcaatca tgacagaacg
   113461 gatccctgcg cgaaccttgt taaaaatgtc atcactctct tcatgatggt cgagatgcag
   113521 gactaacggc aggttgtact cgcgagccag cgattgtact aacgaaatta aacttcccac
   113581 gccaccataa cgatatgtac cgggtgttcc cgccagcata acgggagagc gaagttgtgc
   113641 agcgctctca acaactacct gtaaagtttc aaggttatga atgttgaaag cagggacggc
   113701 atatccctct gactgggctt ttttcagcat gtttttgctt gaaataatgt acatcgcagc
   113761 tcctgttatt tgtcgttcgc cttacaaaat gaagaaagag aatcttcact ttcattttga
   113821 aagcaaacta gcacagcaaa agcagcaaaa agcgtgatgc atgtcg
//
LOCUS       DAPQZD010000002        93343 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPQZD010000002 DAPQZD010000000
VERSION     DAPQZD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.002"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            98..265
                     /locus_tag="RO454_000113"
     CDS             98..265
                     /locus_tag="RO454_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDY0932006.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(373..1458)
                     /locus_tag="RO454_000114"
     CDS             complement(373..1458)
                     /locus_tag="RO454_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDY0932007.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(1470..2774)
                     /locus_tag="RO454_000115"
     CDS             complement(1470..2774)
                     /locus_tag="RO454_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDY0932008.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2786..3139)
                     /locus_tag="RO454_000116"
     CDS             complement(2786..3139)
                     /locus_tag="RO454_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDY0932009.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3150..4028)
                     /locus_tag="RO454_000117"
     CDS             complement(3150..4028)
                     /locus_tag="RO454_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDY0932010.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(4025..5251)
                     /locus_tag="RO454_000118"
     CDS             complement(4025..5251)
                     /locus_tag="RO454_000118"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDY0932011.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(5252..6415)
                     /locus_tag="RO454_000119"
     CDS             complement(5252..6415)
                     /locus_tag="RO454_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDY0932012.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            complement(6499..6861)
                     /locus_tag="RO454_000120"
     CDS             complement(6499..6861)
                     /locus_tag="RO454_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDY0932013.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6878..7783)
                     /locus_tag="RO454_000121"
     CDS             complement(6878..7783)
                     /locus_tag="RO454_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932014.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7945..8949)
                     /gene="trpS"
                     /locus_tag="RO454_000122"
     CDS             complement(7945..8949)
                     /gene="trpS"
                     /locus_tag="RO454_000122"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDY0932015.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8942..9700)
                     /gene="gph"
                     /locus_tag="RO454_000123"
     CDS             complement(8942..9700)
                     /gene="gph"
                     /locus_tag="RO454_000123"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDY0932016.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9693..10370)
                     /gene="rpe"
                     /locus_tag="RO454_000124"
     CDS             complement(9693..10370)
                     /gene="rpe"
                     /locus_tag="RO454_000124"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDY0932017.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10388..11224)
                     /gene="dam"
                     /locus_tag="RO454_000125"
     CDS             complement(10388..11224)
                     /gene="dam"
                     /locus_tag="RO454_000125"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDY0932018.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11331..12617)
                     /gene="damX"
                     /locus_tag="RO454_000126"
     CDS             complement(11331..12617)
                     /gene="damX"
                     /locus_tag="RO454_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDY0932019.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12709..13797)
                     /gene="aroB"
                     /locus_tag="RO454_000127"
     CDS             complement(12709..13797)
                     /gene="aroB"
                     /locus_tag="RO454_000127"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDY0932020.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHSIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13854..14375)
                     /gene="aroK"
                     /locus_tag="RO454_000128"
     CDS             complement(13854..14375)
                     /gene="aroK"
                     /locus_tag="RO454_000128"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDY0932021.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14776..16014)
                     /gene="hofQ"
                     /locus_tag="RO454_000129"
     CDS             complement(14776..16014)
                     /gene="hofQ"
                     /locus_tag="RO454_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDY0932022.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15926..16330)
                     /gene="hofP"
                     /locus_tag="RO454_000130"
     CDS             complement(15926..16330)
                     /gene="hofP"
                     /locus_tag="RO454_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDY0932023.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16320..16760)
                     /gene="hofO"
                     /locus_tag="RO454_000131"
     CDS             complement(16320..16760)
                     /gene="hofO"
                     /locus_tag="RO454_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDY0932024.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16744..17283)
                     /gene="hofN"
                     /locus_tag="RO454_000132"
     CDS             complement(16744..17283)
                     /gene="hofN"
                     /locus_tag="RO454_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDY0932025.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17283..18062)
                     /gene="hofM"
                     /locus_tag="RO454_000133"
     CDS             complement(17283..18062)
                     /gene="hofM"
                     /locus_tag="RO454_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDY0932026.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18182..20734
                     /gene="mrcA"
                     /locus_tag="RO454_000134"
     CDS             18182..20734
                     /gene="mrcA"
                     /locus_tag="RO454_000134"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDY0932027.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20900..21460)
                     /gene="nudE"
                     /locus_tag="RO454_000135"
     CDS             complement(20900..21460)
                     /gene="nudE"
                     /locus_tag="RO454_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDY0932028.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            21780..23915
                     /gene="igaA"
                     /locus_tag="RO454_000136"
     CDS             21780..23915
                     /gene="igaA"
                     /locus_tag="RO454_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDY0932029.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMLFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23980..24648
                     /gene="yrfG"
                     /locus_tag="RO454_000137"
     CDS             23980..24648
                     /gene="yrfG"
                     /locus_tag="RO454_000137"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDY0932030.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24659..25060
                     /gene="hslR"
                     /locus_tag="RO454_000138"
     CDS             24659..25060
                     /gene="hslR"
                     /locus_tag="RO454_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDY0932031.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25085..25963
                     /gene="hslO"
                     /locus_tag="RO454_000139"
     CDS             25085..25963
                     /gene="hslO"
                     /locus_tag="RO454_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDY0932032.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26086..27294)
                     /locus_tag="RO454_000140"
     CDS             complement(26086..27294)
                     /locus_tag="RO454_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0932033.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            27302..27499
                     /locus_tag="RO454_000141"
                     /pseudo
     CDS             27302..27499
                     /locus_tag="RO454_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27597..29321)
                     /locus_tag="RO454_000142"
     CDS             complement(27597..29321)
                     /locus_tag="RO454_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDY0932034.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGLLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            29699..31321
                     /gene="pckA"
                     /locus_tag="RO454_000143"
     CDS             29699..31321
                     /gene="pckA"
                     /locus_tag="RO454_000143"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDY0932035.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(31398..32750)
                     /gene="envZ"
                     /locus_tag="RO454_000144"
     CDS             complement(31398..32750)
                     /gene="envZ"
                     /locus_tag="RO454_000144"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDY0932036.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32747..33466)
                     /gene="ompR"
                     /locus_tag="RO454_000145"
     CDS             complement(32747..33466)
                     /gene="ompR"
                     /locus_tag="RO454_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDY0932037.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            33694..34170
                     /gene="greB"
                     /locus_tag="RO454_000146"
     CDS             33694..34170
                     /gene="greB"
                     /locus_tag="RO454_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDY0932038.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            34267..36588
                     /locus_tag="RO454_000147"
     CDS             34267..36588
                     /locus_tag="RO454_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDY0932039.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMEHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            37026..37253
                     /gene="feoA"
                     /locus_tag="RO454_000148"
     CDS             37026..37253
                     /gene="feoA"
                     /locus_tag="RO454_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDY0932040.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            37270..39591
                     /gene="feoB"
                     /locus_tag="RO454_000149"
     CDS             37270..39591
                     /gene="feoB"
                     /locus_tag="RO454_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDY0932041.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQIASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            39602..39838
                     /gene="feoC"
                     /locus_tag="RO454_000150"
     CDS             39602..39838
                     /gene="feoC"
                     /locus_tag="RO454_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDY0932042.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            40105..40467
                     /locus_tag="RO454_000151"
                     /pseudo
     CDS             40105..40467
                     /locus_tag="RO454_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(40496..41266)
                     /gene="bioH"
                     /locus_tag="RO454_000152"
     CDS             complement(40496..41266)
                     /gene="bioH"
                     /locus_tag="RO454_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDY0932043.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            41304..41987
                     /gene="gntX"
                     /locus_tag="RO454_000153"
     CDS             41304..41987
                     /gene="gntX"
                     /locus_tag="RO454_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDY0932044.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            42046..42621
                     /gene="nfuA"
                     /locus_tag="RO454_000154"
     CDS             42046..42621
                     /gene="nfuA"
                     /locus_tag="RO454_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDY0932045.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42985..44301
                     /gene="gntT"
                     /locus_tag="RO454_000155"
     CDS             42985..44301
                     /gene="gntT"
                     /locus_tag="RO454_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDY0932046.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44411..46495)
                     /gene="malQ"
                     /locus_tag="RO454_000156"
     CDS             complement(44411..46495)
                     /gene="malQ"
                     /locus_tag="RO454_000156"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDY0932047.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVIVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46505..48898)
                     /gene="malP"
                     /locus_tag="RO454_000157"
     CDS             complement(46505..48898)
                     /gene="malP"
                     /locus_tag="RO454_000157"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDY0932048.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWVKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49521..52226
                     /gene="malT"
                     /locus_tag="RO454_000158"
     CDS             49521..52226
                     /gene="malT"
                     /locus_tag="RO454_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDY0932049.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52269..52688)
                     /locus_tag="RO454_000159"
                     /pseudo
     CDS             complement(52269..52688)
                     /locus_tag="RO454_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="catalyzes the conversion of terminal3'-phosphate of
                     RNA to the 2',3'-cyclicphosphodiester; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            52618..53055
                     /locus_tag="RO454_000160"
     CDS             52618..53055
                     /locus_tag="RO454_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0932050.1"
                     /translation="MFCSCKEKLPASVAISRSATWRGNFRELSASVTRMATFATSGRI
                     TLDTVEDEINRLRYNWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSA
                     AGRELFDISRQGKASVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(53037..53795)
                     /locus_tag="RO454_000161"
     CDS             complement(53037..53795)
                     /locus_tag="RO454_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDY0932051.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(53812..54642)
                     /gene="glpG"
                     /locus_tag="RO454_000162"
     CDS             complement(53812..54642)
                     /gene="glpG"
                     /locus_tag="RO454_000162"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDY0932052.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(54687..55013)
                     /gene="glpE"
                     /locus_tag="RO454_000163"
     CDS             complement(54687..55013)
                     /gene="glpE"
                     /locus_tag="RO454_000163"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDY0932053.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            55203..56708
                     /gene="glpD"
                     /locus_tag="RO454_000164"
     CDS             55203..56708
                     /gene="glpD"
                     /locus_tag="RO454_000164"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDY0932054.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(56762..57367)
                     /locus_tag="RO454_000165"
     CDS             complement(56762..57367)
                     /locus_tag="RO454_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932055.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(57370..58137)
                     /locus_tag="RO454_000166"
     CDS             complement(57370..58137)
                     /locus_tag="RO454_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932056.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(58240..59748)
                     /locus_tag="RO454_000167"
     CDS             complement(58240..59748)
                     /locus_tag="RO454_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDY0932057.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(59873..62320)
                     /gene="glgP"
                     /locus_tag="RO454_000168"
     CDS             complement(59873..62320)
                     /gene="glgP"
                     /locus_tag="RO454_000168"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDY0932058.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(62339..63772)
                     /gene="glgA"
                     /locus_tag="RO454_000169"
     CDS             complement(62339..63772)
                     /gene="glgA"
                     /locus_tag="RO454_000169"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDY0932059.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(63772..65067)
                     /gene="glgC"
                     /locus_tag="RO454_000170"
     CDS             complement(63772..65067)
                     /gene="glgC"
                     /locus_tag="RO454_000170"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDY0932060.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(65085..67058)
                     /gene="glgX"
                     /locus_tag="RO454_000171"
     CDS             complement(65085..67058)
                     /gene="glgX"
                     /locus_tag="RO454_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDY0932061.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(67055..69241)
                     /gene="glgB"
                     /locus_tag="RO454_000172"
     CDS             complement(67055..69241)
                     /gene="glgB"
                     /locus_tag="RO454_000172"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDY0932062.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(69514..70617)
                     /gene="asd"
                     /locus_tag="RO454_000173"
     CDS             complement(69514..70617)
                     /gene="asd"
                     /locus_tag="RO454_000173"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDY0932063.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            70809..71402
                     /gene="yhgN"
                     /locus_tag="RO454_000174"
     CDS             70809..71402
                     /gene="yhgN"
                     /locus_tag="RO454_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDY0932064.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(71448..72743)
                     /locus_tag="RO454_000175"
     CDS             complement(71448..72743)
                     /locus_tag="RO454_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDY0932065.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(72650..74746)
                     /locus_tag="RO454_000176"
     CDS             complement(72650..74746)
                     /locus_tag="RO454_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDY0932066.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(74887..76227)
                     /gene="gntU"
                     /locus_tag="RO454_000177"
     CDS             complement(74887..76227)
                     /gene="gntU"
                     /locus_tag="RO454_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDY0932067.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76231..76758)
                     /gene="gntK"
                     /locus_tag="RO454_000178"
     CDS             complement(76231..76758)
                     /gene="gntK"
                     /locus_tag="RO454_000178"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDY0932068.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76897..77892)
                     /gene="gntR"
                     /locus_tag="RO454_000179"
     CDS             complement(76897..77892)
                     /gene="gntR"
                     /locus_tag="RO454_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDY0932069.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(77985..78680)
                     /gene="yhhW"
                     /locus_tag="RO454_000180"
     CDS             complement(77985..78680)
                     /gene="yhhW"
                     /locus_tag="RO454_000180"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDY0932070.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(78803..79840)
                     /locus_tag="RO454_000181"
     CDS             complement(78803..79840)
                     /locus_tag="RO454_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDY0932071.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            80174..80662
                     /gene="yhhY"
                     /locus_tag="RO454_000182"
     CDS             80174..80662
                     /gene="yhhY"
                     /locus_tag="RO454_000182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0932072.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(80723..82456)
                     /gene="ggt"
                     /locus_tag="RO454_000183"
     CDS             complement(80723..82456)
                     /gene="ggt"
                     /locus_tag="RO454_000183"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDY0932073.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            82576..83016
                     /locus_tag="RO454_000184"
     CDS             82576..83016
                     /locus_tag="RO454_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDY0932074.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(83003..83746)
                     /gene="ugpQ"
                     /locus_tag="RO454_000185"
     CDS             complement(83003..83746)
                     /gene="ugpQ"
                     /locus_tag="RO454_000185"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDY0932075.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(83743..84813)
                     /gene="ugpC"
                     /locus_tag="RO454_000186"
     CDS             complement(83743..84813)
                     /gene="ugpC"
                     /locus_tag="RO454_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDY0932076.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(84815..85660)
                     /gene="ugpE"
                     /locus_tag="RO454_000187"
     CDS             complement(84815..85660)
                     /gene="ugpE"
                     /locus_tag="RO454_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDY0932077.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(85657..86544)
                     /gene="ugpA"
                     /locus_tag="RO454_000188"
     CDS             complement(85657..86544)
                     /gene="ugpA"
                     /locus_tag="RO454_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDY0932078.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(86642..87958)
                     /gene="ugpB"
                     /locus_tag="RO454_000189"
     CDS             complement(86642..87958)
                     /gene="ugpB"
                     /locus_tag="RO454_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDY0932079.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEARIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(88355..89068)
                     /gene="livF"
                     /locus_tag="RO454_000190"
     CDS             complement(88355..89068)
                     /gene="livF"
                     /locus_tag="RO454_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDY0932080.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(89070..89837)
                     /gene="livG"
                     /locus_tag="RO454_000191"
     CDS             complement(89070..89837)
                     /gene="livG"
                     /locus_tag="RO454_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDY0932081.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGMPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(89834..91111)
                     /gene="livM"
                     /locus_tag="RO454_000192"
     CDS             complement(89834..91111)
                     /gene="livM"
                     /locus_tag="RO454_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDY0932082.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(91108..92034)
                     /gene="livH"
                     /locus_tag="RO454_000193"
     CDS             complement(91108..92034)
                     /gene="livH"
                     /locus_tag="RO454_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDY0932083.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(92082..93191)
                     /gene="livK"
                     /locus_tag="RO454_000194"
     CDS             complement(92082..93191)
                     /gene="livK"
                     /locus_tag="RO454_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDY0932084.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT        22605 a        25551 c        23311 g        21876 t
ORIGIN      
        1 gctaattgaa tattcctgta tcgttcttaa tgagcagtct gcttatattc atataatcaa
       61 tgaatattaa ttaatatata atacataggg aatgtaaatg aacaaattta ttaaagttgc
      121 actggtaggc gcagtactgg ctacgttaac tgcatgtact ggtcatattg aaaaccgtga
      181 taagaactgc tcttacgact acctgctgca cccggcaatt tctatttcta aaatcattgg
      241 cggttgcggt ccgactgcac agtaagtctc tgacagaaat cggctaacgc cggtttaatt
      301 gcctgacgcc ctcggagtta tccgagggcg tatttgtttc tgcattcttt tcgaaaatat
      361 cgtttacaac gattaaatgc tggcaatact ttcacgcaag atccgcaata tcgtctcttc
      421 accgccgcga tttgggttaa tgcgaatcgc gcaatgttct aactgcggat tcgcctggcg
      481 aaacgttccg gaaagtcgat aaaagagcgg cgggatttca tatttcgact ctgcaccaac
      541 cgggtaaggc aaggcaccga gtttctgcgc ctcttccagc actctggcgg caatcggctg
      601 atgaaactcc acaatcaata ccttcgactg tgcattggca atgacggcgc ttttcacttc
      661 cgccaccgcg ccgccgttaa gcaaagccag caaccgttca gatacccccg cctgcaccgc
      721 gtgcatcact ggcgcgagca ccagaccacg caacacctct aatgcctgcg ccccctgaat
      781 ctgactgccg ccggaataga gcgtggcgcg aatccggcta ataacatcgg catcgcctac
      841 caccgcacca acgccctctg gcccaaatag cttgaagcag gaaaatgtcg acacattcgc
      901 cccgcattcg cagccgattc gcgctacctt catcaccgca tagttgtcat cggttaacgc
      961 tggaacacct gccgcgcgca acgttgccag cacgtctgcc agcacatagc ggtcctgcgg
     1021 ctgctggcgc gtatgctgca ccagcgcggc atcaggttgt tgctcgtcaa caacctgctt
     1081 cagtgccgac agatcattga aatcagccgt aatgagcgtt agccccatct gctcaataat
     1141 gacgcgcgtc gttggataaa ccggcgcgtc atgcaccaga agacgctgcc ccgatttgag
     1201 caaagctgcc agcgccgcac ggatcgcgcc agtacccgcg ccctgtacca gcgccgcggc
     1261 ctgtgcgtga aacgcgtcag ccagcacctg ttccacacgc tgggtaatgc ggggctgatt
     1321 tagccccggc gttaagccca aatcaccgca ggtaagaaac tcgctgccag gaaaatgacg
     1381 gcaaatcgtg tccaccagcg caaactgctt ttgctgcgcc tcaataagcg tcaggctttg
     1441 cagaggaaac gtcttcatag gtgccttcct taagccggaa cgaacaggcc aagccagtaa
     1501 agaacattca gcaggatgcc ggtgatcatt actgccacga ccggtgccgc cattttctgt
     1561 accggacggc ccagggattc gttgaggaag taaatggcaa ccgcgataga gaatccggta
     1621 tagcccgcca ttttgattgc tgcaaagatc gaaccgacca gcaacgccac ttccatcagc
     1681 atattcatgg cgttacggat gttatccgac gcattacgta ccgacgggta gcgccccaac
     1741 catttgccga tagaacgaag cagcaagact tccgccgaaa taaccactgc gcctaatacc
     1801 gctgcaacca tcggattcgg cgagagatag cccaccgcat aaacaaaggt aaagcccgca
     1861 actgcataca cgccggtggc taacgccgtg gtggcaatca acggcacaaa acccagcccg
     1921 cgcataaatt ctgccagtgc cgcctggttg attagtgttt gcgattgttc cggcgttacg
     1981 cctgcggagt acgctttctc cagtgtgaag atcgacactt cactaccggc gaaaatcttc
     2041 atgctggcaa cggcagcaat caatgcaccc acgatggcga tatacggtaa gtttttgata
     2101 atccgtgatg tacgctcttc aaacaccgac ataccgctgg catcgatatc attttcatca
     2161 cgatgacgca ggtcatgagt tattgcgatc cccagcagca tcaccatgcc aataaagatt
     2221 tcgatggatt cagggttaag atgtgggaaa tagcgtacta ccaccacacg agtcatcagt
     2281 accaccacgg cggcgatcag actttgcttc cagccaaact ggtagaaaat cgccaccaac
     2341 gggaacagtg caaaagctga aaccaccggc gagcttaatt cccccaggct acctaataca
     2401 tccaccggta gcgcggtcag cagctggttt actggcaaca gacaggtgag gatcaatatt
     2461 ccccagatag caccaagacc aaacgccatc aggctgttta tcgccagtac gccgaggata
     2521 tcggtaggaa gaaagagtaa ccatgcgttg agcagcccgg ttttcagggt aaaagagatc
     2581 cccaccgagg ccacaaaacc aatgctcaga ccaaaagcga tgctccccgc ctcgcgacgg
     2641 ttcatatagc cttcaatcag ttgtggcagg atcgggcgga taccgtcatg aaaaacggcc
     2701 gccgagcgat gcgccagaag cgatgtcata cccgtcaggc acgccaccac gataatctga
     2761 atatacagat ccatagtcgt gccttttatt ttagatggtt aatcagcatc ggaatcgcgt
     2821 gttcaacgtg ctcaacggaa aggccaaacg ccacttttcc ttcagcgatc attttggcga
     2881 tatgttcgtc tttcgcttta atgcccggct tggcaatggt gcaacttttg ttatagccaa
     2941 tcaccgcgat agcaatcgac aaggctgcgc ccgcaccggt attacacgcg ccgatgtagt
     3001 aatccagttg tccggatttc actttcattg ccgcttccat gtcgttgtga atgaaaactt
     3061 caaaacagcc aggagccgtc gcttcaatgg tttttttaat ctgctcacgc tgtaagcctg
     3121 caacgccaat ctttttcatg agtctgtcct tattggaaaa attgagaggg attttcacgt
     3181 aacatcacat ccacatcggc ctgactgaat cctgactggc gcaattgcgg aataaaggtg
     3241 gtcaataaaa agtcatagcc ataaccaccg ttggctttta aatgagagcg ccgcgtaata
     3301 tccatcgaca gcatgacgcg gttcagcaac ccacggtcac gtagcgcatg gagcatcgca
     3361 atgcgctttt cgtccgggta gtaactgttc ttgccgatgg tgtcgaactg cacatacgcg
     3421 ccgagatcga tcatcttcaa aatgttgtcg agattgtctt tcagatcgca gtggccgacg
     3481 gtgacgcgcg aaagatcaac tccgtgggct tgcaacagag ccagttgctc cagccccatc
     3541 gtgctgaacg acgtatgcgt ggagatcggg cgtccggtct ggttatgcgc cagcgcggcg
     3601 gcaataaaca ccttctcttc cagcgacgta atcttccctt cgctggagcc gatctccgcg
     3661 ataattccgg ctttcaggtc agtgccatcg ataccctgct caatttcatc gaccatctcc
     3721 tgcgccagtt cctgcacgct gcgggtcgcc acatgttccg ggaaaaacgc gtcctggtaa
     3781 taaccggtac agcccaccac gttgatcccg gtttcgcgca ttacatcgag cataaattgc
     3841 gcattgcgcc ccatgtaacg gttggtcatc tcaatcacat tacgcacgcc ccgggccatc
     3901 aggtcgttca tctcctggca aatgaacgca tactgatcaa ggcggcagtc cacgttgttt
     3961 ttaaagccgg aaagatcaat atgcagatgc tcatgggcga gggtgtagcc cgtcggatca
     4021 aaactcatag ggtgtctcct gcaaaccgga aggaggaaag aaaagagcga ccattttgtg
     4081 gcggtggcgc acggaaaaat tcacacacgg tagcccccac atcagaaagc gtggtgcgca
     4141 caccgagctg cgtagcgacc agtccttgct gataaaccag cactggcacc acttcgcggg
     4201 tatggtggct gtgaccgatg gtcggatcgt taccgtgatc cgccatcacg accaggcaat
     4261 catctggctg catcgcctca acaagccggg caaggttacg gtcaacgacc tgcaaacgtt
     4321 cggcataacg tgcgacgtct tctgcatgac cagcgaggtc ggtttcctgg atattggtac
     4381 aaataaacgc cgtcggatag gtgttaaatt cgttgagggt gatatccata atccgctggc
     4441 tatccaccag gttttgccag ctcacgccat aaggattgct gacgatatct gccaccttac
     4501 ccaccagcac ggttggcacg cccgcttcat acagtttttg cggcacctgc actttttcat
     4561 cgacaccgta gcccatatgt acgacctgga aaccgttgtc ataggcgcca gaacgcggcg
     4621 cattgatacc aataaagcgc ccttctttgc tttctgcggc atcgaggatg cactggctgt
     4681 cggttaacag gccaccaaat gtaatgaccc gaccgacctg tacctgctca cgcacgatac
     4741 gacccatttt gattgcgtcg tcaaaagaga tcacagagag attggcggtg atgttataga
     4801 cctgacctaa atccgcctcg agattatcgc caatcgcaac cgcctgattg acccacagaa
     4861 attgcagatc atcgccacga cgctcaacct gccagccagc ggaaactaat gcctgctcaa
     4921 cacggtcaat cacatcacga aagggcatcc gcagcggcgg taatgggcgc gtacctaaaa
     4981 tttcctgatg ccccataaag gtatcgccac cttcatgttg cagctctgcc acgccccagg
     5041 ttgcggagtc tgacggctgc atatcgccag gcgcataacc caatgcgttg attagcccca
     5101 gcgtctccag cgttggtagc tgcaaatgcg gcaactggct caggatgtga ccacaggtat
     5161 tcgccccggc atcctgcgga cgcaccaacg tgacatcttt cattgcccct acgccaaagc
     5221 tatcaatcac taacaccaca aatcgcgcca tttacgcctc cagagggttg ccgaggctgt
     5281 cataacgacc aactatttct ggttcgccat ggtgaatacc ggacaccaac accacatcgc
     5341 tacgggtgat aaaaatctgc gtgcgaaaac agagcaccac tgcactgctt accggatgct
     5401 cgcctgccag cggcagggtg tagtcgatac tgctgtcatc cacggcgttg agatatgttt
     5461 cagtaattct ttgattttct ggcgtaaaca ccagcgcatg ttgcgcgtga ccacgacggt
     5521 agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg atgggagatt tcacttaacc
     5581 agagcatcgc gatacgttca ggctgatcgc cccgctggtt tgccggaata gtgcccgtca
     5641 gcgcatgacc gggttcggta tgagtcacac cgtattccac cagcaatggc agcgacgtgc
     5701 agctggtcgc tgaaggcgcg ttcagttgct caatcgcaat accagatttc gccagttgat
     5761 cccgtgcttg taccagcgtg tgaagattcg gtgtcggcaa aactttcccg gcagcctcat
     5821 cccaaagcag gcaagggaaa tgggtaagtc ccgccaaatg tagccccggc agattctgga
     5881 tttcagcaac cacctcgtgc agcgaatgct gtacaaaacc gctctcctgc cccggataaa
     5941 gaaaatcatc gtcgctataa actttgagca gcacagactg cacacgtccg gccttcaccg
     6001 ctgccgcaga aatttcccgc gctttgtcga gggtaaacac agtgatgacg tcggttccct
     6061 gttcaacggc gtcagaaacc tgatgacaag ggatttgtac cagatgcccc tgatgcgcta
     6121 ccggcaaacc ggcgcggcgc atgactcgcg cctctttgta atccaccgcc acaatgccgc
     6181 tgtagcccag agccagcagt ttttctgcca gccacggatt acgaccgaat tgtttggtca
     6241 tcagatacag ttcaatgccg taaagccgcg ccgtctcaat cagccgcttg ccgttttcca
     6301 gaacttgatc cacgtcgatc acccaactgt ccggggcgat cttgccctgc tgccacaaac
     6361 ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa tgcttctaca aacatcttgt
     6421 tgcctcgttt ctgttccttg aattaaatat tcattttttt gaatatttag gttgtacgac
     6481 gtggatttat cgggagcttc aaacctcttc gttagcagcc atccataatc cataaaggtt
     6541 ggccagcaaa tagccttctt cacacgggtt aacttccagt gcaaattcct tcaacaatac
     6601 ctgatgcaat tgcacaacgg cttgccagtg gctggattgc gccagttccg ccagcaattc
     6661 gtcatccagc ggctctattt cttcaccacg gcgactgcgc atcagtgcac tcgccatatg
     6721 tgtcatcgcc atcgttcctt gctcactgcg caccggtaga tggcactctg tttccagtac
     6781 attgacgacc tgcatcatgc ctttgcagat gtccttatca ataacgcctg catcgcaaag
     6841 caggttgagt ctggtttcca tatcagatgc ctttcgttta ataactgggt tcctgttccc
     6901 cactgactac tcgctgttga tgagcaagca gggcatgttt atcaacaact gcacgcagca
     6961 gttgttgcat tgggtaatca tcgactcgcg tcaggaccac ggcttcggtg gcctgtaaaa
     7021 atcgcggatc gtctgtgagc ggcgtcgctt ctaatcccag catggtcagt tcgttttccg
     7081 ccactacgtt ccagataacc gcgtcaacat cgcctttaac aatgcgttgt aaactctcgt
     7141 gataagagag atcgactcgt tccacatcac tatcgccaaa aaagacatcg gtcatgattt
     7201 tctgatccgc cgaacggtta tccagcccca cgcgcttcac gtttgcggac tcgcctttac
     7261 ggcaaatcaa ctggtgctcg ccaacgtagg tatgcggtcc caactccagc gcgaggcata
     7321 atcctttttg cgtgagataa ctttccgccg ccagtcgcga aaccaccgcc atgtcataca
     7381 cgccgttaag taaacactcc acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag
     7441 gaatgccatc aaactgggct ttcaatccgc tcgccaggcc ttcgtacaaa cgggtatagg
     7501 gcaagggcat cgcacatacc acgttgttga tatccacatg agtcagcaat gctttgttat
     7561 ccatctcaac cagataactg ccattgcgcc cacggcgttc aatccgtatc gctccgcttg
     7621 attccagcgt tttcaacgcg gcctgcgtca ggccaaccga tgaacggcat tcagttgcca
     7681 gctcatctat ggttttcagt cgattaccgc acttttcacc caacaaataa cgggccagtg
     7741 tcgtgataac gacgccttct tttttgataa acgttcgacg cataatatat tttcaataaa
     7801 atgaatgttt atatcttcat gaatttgaag ataaggcgca acagcgagat gtggaaacgg
     7861 cgaggcgctt cacatttttt cggattattg caaatgagga taaaaaaacc gggtttcccc
     7921 ggtttttttg gcattaatgt taaattacgg cttcgccaca aaaccaatcg cttcgtacac
     7981 cgcttttagc gtacgggaag cgtgtgcgct ggctttttct gcgccgtctt tcatcacctg
     8041 ttgcaggaag gcttcatcgt tgcggaaacg gtggtaacgt tcctgcaatt cagtcagcat
     8101 accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc cttcgaactg
     8161 tttttccagt tccgggatgc tctggcccgt taccgcagaa aggatatcca gcaggttgga
     8221 aacgcccgct ttgttctgca catcgtagcg aactactggc ggctcgtcgg agtcagtgac
     8281 cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga taacgttatt
     8341 gcggttatcg tcagacttgg acattttctt ggtcggctcc agcagcgaca ttacgcgcgc
     8401 gccagatttc ggaataaacg gctctggcac cttaaagata tcgccgtaca gcgcgttgaa
     8461 acgctgggcg atatcgcggc tcagttccag gtgctgtttc tggtcttcac ccaccggtac
     8521 cagatttgtt tgatacagca ggatgtccgc tgccatcagc accggatagt caaacagacc
     8581 agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgcgtca tgcggctcag
     8641 ttcgccgaag taggtatagc agttcaatgc ccagcctaac tgcgcatgtt ccggcacgtg
     8701 ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat acaacgccag
     8761 cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga tcgcatgttg
     8821 gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact gacgcagcgc
     8881 acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa aaacgatggg
     8941 cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt
     9001 catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg ccgtagttat
     9061 atccgtaggt taagccaact gatgggcaac ctgccgcttt tgctgcctga atatcattgc
     9121 gtgagtcgcc aacaaacagc atctgttgtg gagcaatgcc catccgttca gccaccagta
     9181 acagcgggtc cggatgcggt tttttgtttt gcacatcatc accaccaatc accacgctga
     9241 agtatttggc gatatctaag gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg
     9301 tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg gcaacatgcg
     9361 ggaacaaaaa cgtcccctct tcggcaacct cgccatagta gcgatcgaac agtttacgca
     9421 gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggttta cccattgttt
     9481 tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc agaatatctg
     9541 cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacaggcaac tccagcgcat
     9601 acagcgccat atctaccgca gcagcaagac cgggtgcact gtcaaccagc gtaccgtcga
     9661 gatcaaaagc gacgccgcga atatcttcaa acttattcat gacttacctt tgccagttca
     9721 ctgcgcattt catcaatgac ttttttgtag tctggctggt cgaagattgc cgaaccagca
     9781 acaaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt cacgccaccg
     9841 tccacttcca gtcgaatgtc aaagccagac tcgtcgatac gacgacgtac ttcgcgcagt
     9901 ttatccagtg tttgaggaat gaaagactga ccgccgaaac cagggttaac agacatcagc
     9961 aggatcacat ccagcttgtc cattacataa tccagatagc tcaatggtgt cgccgggtta
    10021 aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc
    10081 tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca
    10141 atgcgatcga cgggtttcac catcagatgt acgtcgatag gggcggtaat gccgtagtta
    10201 cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg gttatccatg
    10261 acgtcaaaat gcacgacatc tgcacccgct gccagggctt ttgcggtatc ttcacccagg
    10321 cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat ccgcttctcc
    10381 ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt
    10441 ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg acgacatgca
    10501 attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag atcagcactg
    10561 gaatatggcg atcaaccaga ccttcagcga tctccgccag atgcgcttgt tgttcaagcg
    10621 taaaactgtt tgtgtgatac gccgtaaagt tggcagtcgc agacagcggc gcataaggcg
    10681 gatcgcaata gacgacagat gcatcatctg cgcgcgccat gctatcggcg taagactcac
    10741 aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct tccgggaaat
    10801 agggtttttt gtagcggcca aacggcacgt taaactcacc gcgcagattg taacgacaca
    10861 ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg aacggatcct
    10921 ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt gtttcgggaa
    10981 caaatagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca atgttataga
    11041 gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg gtgttgagaa
    11101 acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc aaatgccgtt
    11161 taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg cgatttttct
    11221 tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga cagcatccag
    11281 cgcttcaggc aactcccgca atgataaacc attgcgggag ataaccttga ttacttcaga
    11341 tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctggac atctgctggc
    11401 aatgtagata ccgctttttt cgcctcttct ttcgaagcat acacgccaga aaccaggaca
    11461 taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag attctctttc
    11521 ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag agtgtaatgg
    11581 ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc agcggctggt
    11641 gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt
    11701 ggtgctggcg tagaagtcgc agccggtgcc tttgtgctcg ccacaggggc agctggttca
    11761 gttcgcttcg gcgtctgtgc gactggcttc ggttccgttt tcacggttgc ttgcggtttt
    11821 ttcggttcaa tcaccgcctg ctgacgagct gggcgcgtag tggccggacg ttcagcggtt
    11881 tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgaa caggcgcaac cgttgcgggt
    11941 tcggtcggca atgtagaatt gaccgccaca ttgttcaact gttgctgatt ttgtggctgg
    12001 gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc cgttgccgcc
    12061 ggggtttgcc cttgagtcgg cgtagaagag atcggcggca gagaaacatc ctgctgagta
    12121 ttttccgcag acgtggttcc cggcgctggc tgcacgccat tggcctgatc ggtcgcattg
    12181 cctgcaagat caatactctt ctcgccagac gcagtttgat cgctggaaga ggtcgagggg
    12241 gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat gccgacaccc
    12301 atcatcatat actgacgaga agcgggttta ctggctgctt ttttacgctt acgcggacga
    12361 cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc ttcttcgatt
    12421 tccggttcct cattacgcgc tttttgcgca cgagtcggac ggcgatcgtc agtgtcatca
    12481 agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc agaacgttca
    12541 gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag ctcgtcttct
    12601 ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac gttgcaatta
    12661 acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt acgctgattg
    12721 acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa cttcactctt
    12781 accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct tgtcacgcag
    12841 catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg gtaacccagc
    12901 ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaaccgcc cgagacgttc
    12961 cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta accaattgcc
    13021 ataccccatt tcagcttcaa tggaatgacc aaaggtgtgt cccagattca gtaaagcacg
    13081 taacccggtt tcgcgctcgt cggcggcgac aacttctgct ttcagctcac aacaacggcg
    13141 aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat tctcttccag
    13201 ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg ccagccccga
    13261 cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca ctgaagcggg
    13321 ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag ttttgccgcc
    13381 aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac gtacaccgcg
    13441 ctgataactc gccgccgcga agccggtcag atcacccact acgccgccgc caagcgccac
    13501 cagagtagta tcgcgaccat gcggcttttg taacaatgcc gtaaagacgg tatcgagtac
    13561 agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt taacacccgc
    13621 ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt cgttggtgac
    13681 caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat taaacaaacc
    13741 agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc tctccataac
    13801 gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca gaattagttg
    13861 ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc atcagtacga
    13921 atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa cgcttccaga
    13981 acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg ctgcgtgcgt
    14041 gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc ggaaagacgg
    14101 ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat accctgtttc
    14161 tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc gccttctaaa
    14221 tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc ggaatcgtaa
    14281 aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc ggcacccata
    14341 ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact aagactattc
    14401 gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga aacctcataa
    14461 gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg gcaactgaat
    14521 aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc taccaccgct
    14581 ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc cgcctgaatg
    14641 ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat cggtactcct
    14701 tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcgta aaaacattac
    14761 cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc
    14821 gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc
    14881 aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc
    14941 caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat caatcgccag
    15001 cacttcgcca tctgcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt
    15061 cagccggatg cgaccttttt gtaacaccgt gggcgtgact tccatcccca ggacggcctc
    15121 tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct
    15181 cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac tggcgataat
    15241 atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagtaaac gtccgttgat
    15301 gcgtccaatg ttaaaaccga tatgcgttgt cgccgtcgct acggagaggt cgctgccaag
    15361 cgtggtgact tgcccaacgc caccagcttg ttgcgcatcg gccagcgtcc atttcacacc
    15421 taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc
    15481 gaccggcaga tccatttgcg atacccactg ttcaagcgtg cttaacgccg ttttgttatc
    15541 tcgcagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag
    15601 cttctccccc gctttcgcca gctctccggc gtcggcgtat tgcagagtaa tattgcgatt
    15661 ttccagcggc agatttgcct gcgcccgcgt ctgctccgcc tcctggcggg cgatattgtc
    15721 attctgccag gcaacggaat gcaccgagag aatgttgccc tcctggcgcg ttatcaatcc
    15781 ggcgcttttc actacagttt gtagtgcctg cttccaggga acgtcagtta aatgtaacga
    15841 caccgtaccg ctgacgtctg gcgaaaccac caggttcaac ttctcctgtt cagccagcgc
    15901 ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc
    15961 cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat
    16021 ctccttgccg ttgccacaac cagtgtggcg gttcgcagtt tgtcccggtt cccagcgtta
    16081 gtgcgtctgg cgtcagctgt aagatcgtcc agccgttttc cagcacatcg ttttgctgca
    16141 cccgtcgcca ttttttttgc ccatctttta ttacaccgat gatgcgctcg cctcgcccta
    16201 ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct tccggcggtt
    16261 taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac aaccagcgtt
    16321 taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa ggtcatcacc ttccacgctt
    16381 aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc cgacggcact
    16441 gcttcccaca gcgttttcaa cgccaactca ccaccctgcg cggatggatg ccaggaaacc
    16501 agttgcgcgc cggataactg aaaatccagt ggcgaaaagg gcagcgtttt ttcttcgctg
    16561 aagggagtgg cgtctaccag gcgatacagt gcagcccact gctggtgatg actcgcccgt
    16621 agtcgaatta atgcgtcgcg ctcttcctgg tgtgtcgatg acagaaaaat gagcgtaact
    16681 aacaccagca accagactgc ccagcaaagc tggcggaggc ggagtgatgt ggcgaaccac
    16741 cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa attgccagcg
    16801 tccctgcgca tcctgctgcg tggccccccg ctgattgaga tgaaaagaag catcctggcg
    16861 gagtgacgtt tccagcgcgt ttaacgcggt aatgcttgtt gtaagcccct tgatctccag
    16921 cgttccctgc tgccagctta tcgttgtcag ccaggcatgt tcgggtaaaa gcgccgctag
    16981 tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgac gttgcaaacg
    17041 ctgttcgcgt aactgctggc gttccagcaa acgtggtttc gttatctgta agctgcgggt
    17101 gagttgttgt tccgcctgaa gcaaaacggc gtttacgcgt gcttcggcat tgcttgtcag
    17161 acgcagtatt agcgttatcc cgacggccag cagcaaaggc gcaacgaaca tcagcaacca
    17221 gaaacgaaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa ttggcgggtt
    17281 catcagtaca ccttcccaag cgccagcccc agcgcgatgg caaagtctcc acctggtggc
    17341 ggtagcggcg gctgacgaac agaaaccgcc tcccaggggt caaatccgcc ttcgccacat
    17401 agcgcgacgc tttcgggatc aacagataac gctgccgcca gctccttcgc gctagtcatc
    17461 cccaccgcca gtttgcgccc ccagctatag cgcgtcgccc acagccactg ttcgttatca
    17521 cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaatcgctg taatgcactg
    17581 gcatccggag tgatcgcgct aacatgaaca cgcaaccttt ctgccagagt aagcagcgtt
    17641 gccagctctt tgctttgcgc ggcagtcacg ttataggcgg gactgagtga gtcttcgctg
    17701 taatcgaagc gcagggagtc cggatccata tccagttcac gggccatcgt cccggacagc
    17761 caggccattt gctcccgctc accgagggac atcgacgggc gtggaaatga ccgctgtaat
    17821 gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc gcgactccac
    17881 ggtaacaacg tgttagccag ccgctgcaca tcaacaatcc gcccatcttt gataatgtcg
    17941 ttctccagtg gcaaccgcca ccagcggtgc aataagcatt cttttgcgct ccgcacgata
    18001 gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagtt cttaaatgcc
    18061 attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg tctggcttgc
    18121 ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg ggaaatttcc
    18181 agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc tgggagcagg
    18241 ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg cgacattaaa
    18301 agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc tgattgctca
    18361 atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccgccgg agatggtgaa
    18421 agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg acccggtggg
    18481 gatcttccgt gcggcaagcg tggcgctgtt ctccggtcac gcgtcacaag gggcgagtac
    18541 cattacccag cagctggcga gaaacttctt cctcagtcca gaacgcacgc tgatgcgcaa
    18601 gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctaacgaaag acgagatcct
    18661 cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg gtgctgcggc
    18721 acaagtctat ttcggtaaaa ctgtcgacca actgacgttg aacgaaatgg cggtgatagc
    18781 cgggctgccg aaagcgcctt ccaccttcaa cccgctctac tcaatggatc gtgccgtcgc
    18841 gcggcgtaac gtcgtgctgt cgcggatgct ggatgaaggg tatatcaccc aacaacagtt
    18901 cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga ttgctttctc
    18961 tgcgccatac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg gcgaaagtgc
    19021 ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc aggccgcaca
    19081 gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc gcggcccggc
    19141 aaatgtgctg tggaaagtgg gcgaatcggc gtgggataac aacaagatta ccgatacgct
    19201 gaaggcgctg ccgacctatg gtccgctgct gcctgccgca gtcaccagcg ccaatcctca
    19261 ggaagcaacg gcgatgctgg cagacgggtc gaccgtcgta ttgagtatgg aaggtgttcg
    19321 ctgggcgcgt ccttaccgtt cggatactca gcagggaccg acgccgcgta aagtgaccga
    19381 tgttctgcaa acgggtcagc aaatctgggt gcgtcaagtt gacgatgcat ggtggctggc
    19441 gcaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg gtgccgttat
    19501 ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgagcca ctcaggcact
    19561 gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg ataaaggtct
    19621 gacgctggca agtatgttga acgatgtgcc aatttcccgc tgggatgcag gtgccggttc
    19681 tgactggcag ccgaagaact caccaccgca atatgccggt ccaattcgct tacgtcaggg
    19741 gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg gcgtcgacta
    19801 cgctgcagaa tatctgcaac gcttcggctt cccggcacaa aacattgtcc acaccgaatc
    19861 gctggcgctg ggttcagctt ccttcacccc aatgcaggtg gcgcgcggct acgcggtcat
    19921 ggcgaacggc ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa acgatcaggg
    19981 cggcgtaatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata ttccggtgat
    20041 ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag atgtcgctat
    20101 ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg caaatcaggc
    20161 gttagtggcg aagaccggcg cgcaggagta cgcaccgcac gtcatcaaca ctccgctggc
    20221 attcctgatt aagagtgcct tgaacaccaa tatttttggt gaaccaggct ggcagggtac
    20281 tggctggcga gcaggtcgtg atttacaacg gcatgatata ggcggaaaaa cgggtaccac
    20341 taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga cctcggtctg
    20401 gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg gagcgattaa
    20461 agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat gggacgctta
    20521 tatgaaagcc gttcttgaag gtgtaccgga acagccgctg acgccgccac cgggtattgt
    20581 gacggtgaat atcgatcgca gcaccgggca attagctaat ggtggcaaca gccgcgaaga
    20641 gtatttcatc gaaggtacgc agccgacaca acaggcggtg cacgaggtgg gaacgaccat
    20701 tatcgataat ggcgaggcac aggaattgtt ctgattaaaa aggcgcttcg gcgccttttt
    20761 cagtttgctg acaaagtgca cttgtttatg ccggatgcgg gtgaacgcgt tatccggcca
    20821 acaaaaaatt gaaaattcaa taaattgcag gaacttcgta agcctgataa gcattatgca
    20881 tcaggtaaac ttcacgcatt tacactcgcc cctgcccttt caaccattcg cgcacgagga
    20941 acagcgcgct gacattacgc gcttcgttga agtcaggggc ttccagcaaa tccatcatat
    21001 gcgccagcgg ccagcgcacc tgtggtaacg gctctggctc atcgccttcc agtgattccg
    21061 ggtagagatc ttgcgctacc acgatattca ttttgctgga aaagtaagac ggtgccatgc
    21121 tgagcttctt caaaaaagtc agatcgttcg caccaaatcc aacctcttct tttagctcgc
    21181 ggttagcggc ttcaaagacg ctttcacccg gatcaattaa tcctttcgaa aaacctaatt
    21241 cgtaggattc agttcccact gcgtattcgc gaatcaggat caggtgatcg tccacaatcg
    21301 gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca cgccgcacgc
    21361 cattgctgaa ctccagatcc acgctttcga cggtaaacag tcgggaacgg gctacagttt
    21421 caacattcag aatggtgggt ttttgtaatg atttgctcat cgtgggatct atgctgtgaa
    21481 atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc gcgttattta
    21541 catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa taggttgaaa
    21601 taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc agcggttact
    21661 gtgatgtgct taatgatgct caagttaaac tccacgcttg ccgatagcca accgcagaat
    21721 catgtattgt gtccggtgcg actgaccacg cctgacagac taagtaagat ggggaaagca
    21781 tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt gcgggatggc
    21841 tgataaaagt gcgatccaga cggcgtcagc tgccctggac caacgccttc gcggatgcgc
    21901 aaacgcgtaa actcacatct gaagaacgta gcgccgttga aaattatctt gagagcctga
    21961 cgcaggtatt acaggtgcct ggcccaacgg gagccagcgc ggcaccgatc tctctggcgc
    22021 tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacgcgt tatggcatct
    22081 ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc cacctgcccc
    22141 ctttctggga acagtacatc aacgatgaga ataccgttga actgattcat accgattcgc
    22201 tgccgctggt tatttcactc aacggtcata cgctgcagga gtacatgcag gaaattcgcg
    22261 gctatgcctt gcaacctgtt ccgtcaacgc aggcgtcgat tcgcggggaa gagagcgagc
    22321 aaatagagct gctcaatatt cgcaaagaaa cgcatgaaga atatgcgctg agtcgtccgc
    22381 gcgggctgcg cgaagcgttg ctgattgtcg cctccttcct gatgctcttt ttctgcctga
    22441 ttaccccgga tgtctttgtt ccgtggctgg caggcggcgc gttactgctg ctgggcgcag
    22501 gtctgtgggg gctattcgcg cccccggcaa aatcctccct gcgggaaatt cattgtctgc
    22561 gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag atcaacaata
    22621 tttcactcgg tattatcgac ctggtctatc ccgcacactg gcagccctac attgctcagg
    22681 atctcggcca acaaaccgat atcgatatct atctcgaccg ccacgtagtg cgtcaggggc
    22741 gatatctttc gctccatgat gaagtaaaaa actttccgtt acagcactgg ctgcgcagta
    22801 cgattatcgc tgcgggctcg ctactggtgc tgtttatgct gttattctgg atcccgctgg
    22861 atatgccgct gaaattcacc ctctcgtgga tgaaaggcgc gcagaccatt gaagccacca
    22921 gcgtgaaaca actggctgac gctggcgtgc gggttggcga tacattgcgt attagcggta
    22981 cgggaatgtg taatattcgc acttccggta cctggagcgc gaaaaccaac tcaccttttt
    23041 taccgtttga ctgctcgcag atcatctgga acgacgcccg ctcattgcca ttaccagaat
    23101 ctgaattggt caacaaagcg acggcattga ccgaagcagt taatcgccag ctgcacccta
    23161 aaccggaaga tgaatctcgc gtcagcgcct cattacgttc agcaattcaa aaatccggca
    23221 tggtattgct tgatgatttt ggcgacatag tactgaagac agcggatctg tgttctgcca
    23281 aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt aaagactggg
    23341 acgcgctggt aaaacgcgcc aacgccggga aactcgatgg cgtgaatgtg ttattacgcc
    23401 cggtgagtgc ggaatcgctg gataacctgg tggccacctc taccgcgccg tttatcacgc
    23461 atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcggg ttcctgattg
    23521 tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca ctttacgact
    23581 atccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg atgcatacgc
    23641 cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga cgccaatggt acgcagcata
    23701 ttggccttca tccgatcccg gatcgttccg gtctgtggcg ctatctcagc accacgctgc
    23761 tgctactgac gatgctgggt agcgccattt ataatggcgt acaggcctgg cgtcgttacc
    23821 agcgtcatcg tactcgcatg atgaagattc aggcctatta tgaaagctgc ctgaacccgc
    23881 aactgatcac cccttccgaa agccttatcg aataacacgt ttgcgcggca ggttatgcta
    23941 ccctgtcgcg caaattgctt cactctggag atttccctca tgcatatcaa cattgcctgg
    24001 caggacgtag ataccgttct gctggatatg gacggcacgt tgctcgacct cgccttcgat
    24061 aactatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg agttacgcca
    24121 caggaagcga tggaatatat gcgccagcaa tatcacgacg tacagcatac gctaaactgg
    24181 tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat gaccaccgag
    24241 atgggaccgc gtgccgtact gcgtgaagat accattccgt ttcttgaggc actgaaagcc
    24301 agcggtaaac agcgaatttt gctcaccaat gcgcatccgc acaacctggc ggtaaaactt
    24361 gagcataccg gtctggacgc acaccttgat ttattacttt ccacccacac atttggttat
    24421 ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct taaagctgaa
    24481 agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca atttggtatt
    24541 cgttactgcc tcggtgtgac taatcctgac tccgggattg ccgagaaaca gtatcagcgc
    24601 catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag gagacgccat
    24661 gaaagaaaaa cctgctgttg aggttcgact ggataaatgg ctatgggccg cccgttttta
    24721 taaaacccgc gcgctggccc gcgaaatgat cgaaggtggt aaggtgcatt acaacgggca
    24781 gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc gccagggaaa
    24841 tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg ccagcgaggc
    24901 agtcttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgctggc
    24961 acgtaaactt aatgccttaa ccatgccgca tccggaccga cgcccggaca aaaaagagcg
    25021 ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg caagagagat
    25081 gattatgccg caacatgacc aattacatcg ctatctgttt gaaaactttg ccgttcgcgg
    25141 cgaactggta accgtttcgg aaaccctgca acagatcctt gagaatcacg attatccgca
    25201 gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa ccgctacgtt
    25261 gaagtttgat ggtgatatca ccgtacagct gcaaggcgac ggtccgatga atctggcggt
    25321 tattaacggt aacaacaacc agcagatgcg cggtgtggcg cgcgtgcagg gcgaaattcc
    25381 agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca ccatcacccc
    25441 gagcgaaggc gaacgctatc agggcgtggt tggtctggaa ggtgataccc tggcggcctg
    25501 cctggaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg
    25561 cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg caggtaatgc ctgcgcaaaa
    25621 tgcccagcag gacgactttg accacctggc gacgctaacc gaaaccatca aaaccgaaga
    25681 actgctgact ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac
    25741 ggtttacgat ccgcaggatg tggagttcaa atgcacctgc tcgcgtgaac gttgcgccga
    25801 tgcgctgaaa acgctgcctg atgaagaagt cgatagcatc ctggcggaag atggcgaaat
    25861 tgacatgcat tgtgattact gcggtaacca ctatctgttc aatgcgatgg atattgccga
    25921 gatccgcaac aacgcgtctc cggcagatcc gcaagttcat taatgagtat gtccggcaga
    25981 gaggactctg ccggatttta cgacgtaatg gcctgatgcg ctgcgcttac caggcctatg
    26041 aattgacatc ctccacgccc tgaaggacgt ggatccctgc tacgttcagg ctgtcgcctg
    26101 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatct ctccacaggc
    26161 aagaacggcg tgccccgccg ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt
    26221 atatccacat acctggcatc tgaatttact ttgtgacagg cggttttctt tcgctgtatg
    26281 accacagcac gcgcaacgct ggcttgtgta cgctggcgga acagcaagca cctgaccgcc
    26341 actccagagc tgcttgtgct caagctggcg gcgcatttca taccagccct gatccagtat
    26401 cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc
    26461 tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcattg cgtggttttt
    26521 gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
    26581 cagccgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
    26641 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
    26701 aaagactgtg ccatctgaca gcgtggcgag tttagccacg ccagcatcca gcccgaccat
    26761 tgatgctgaa gggtgaaccg gagttgatac ttcactttct gtctgaatac tgatgtacca
    26821 cttaccgcag gactggctga cagtgacatt tttcacaact cccgtgacct gacggctatt
    26881 ccggtagcgc atccaaccaa gtttcggcag aaaaatacgg ctgttttcct gatcgagctt
    26941 aacgccctgc gggtagcgga atgcatcatt ctgtccccgc tttttgaatc ggggaaaagc
    27001 agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg tctttcagtg actgttgcaa
    27061 tggctgtgag ggagaatctt taagccattg cgtttcagtg gcatttttcc actcaaccag
    27121 ccaggaagcc attttcccgt aagggatgta tttattaccg gcctcatgat tctcattctg
    27181 aagcgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg
    27241 ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc tgtagtcgtt tcacaggtca
    27301 aattatattt tggtctatga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
    27361 cctgatgcat gttcacctgg tctttgtcgc caaataccaa cgccagattt ttgaccatga
    27421 cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg aagctgaacg
    27481 ggttgaaatg gatggcgaac ttatatcccc gccctgaagg acggggtttt accgcgcacc
    27541 ggataaatcg caagttattt tggtcagata cagcgtcgta tccgttaaaa gcgttgtcac
    27601 ttacttaagt ttatttccag cgtttcacca tctaccgtca gatcgcgcca cgctttcggc
    27661 ctcgtcccca gttttccatc cttcgcagct gtgagtaatt tttctttcgt tattttgcgc
    27721 ggaagtaaac tcatatcaag cgcgtcccat tcttttctgg ctgagtcaaa gccatagaca
    27781 atgactctgt catcattaaa agcaaacagg atccgctccg cctgaccatc actattcaga
    27841 tcttgctcga ccagtacgca ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc
    27901 tggattaagg ccgaccagaa tgtcgcatcc ggtttaccag aacctggggc aattaacaca
    27961 ttatcggcta atactttttc cgatacctgt tcctgaagat gttgctctcc atctaacgcc
    28021 atcagcagat cccgcgcgcg tttcgggtct ttcatatact cggcatcgct tttcagcgac
    28081 tcaagcgccg cacgtccata gcgaccgctc tgttcgagca tgtagatagt tacctggtct
    28141 ggcgtgtttt taccgctctg ataacgcgcc atatgactgt tcacgctaat gcgcatactg
    28201 tccagcaccg gcgaattaag aagcaccagt attaccaaca ccaataacga aaccgcaagg
    28261 ttcactttgc cctgaagatc aaggggattt tgccctttac gccagacgat actgacaaaa
    28321 taccccagcg accataccag taacaccagc accgccagcg cgccctgcaa gcggtcgaca
    28381 gtccagccgt actgagcgac ccgcagccat aacgcccagg cggcgacgaa cacatacagc
    28441 ggagcaacca gcaaagcggt tttaatcagg caacgcaatg gccccgtcca gggaagtgac
    28501 gctttttgcg gatcgcggac aatagccatt aagatcaatt gcaaaaaggc cagcgtcaac
    28561 agcaacccgg cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa cggcagggtg
    28621 ataataaaca tcagggttag caatgatact aacggcagca accccgtggc gattaacgtg
    28681 aacaactttt gaatagagtc gattaaacgt gactgtgttc gcgcgaggat caccgccagc
    28741 gcggtaacca gacctaacgt gagataaata aaccagtcgg ttgcaaaaaa gagcgttttg
    28801 aaaaacgtga tgccaacaag tttaaacaac tcactccaca gtaaaagtac cagccacgtt
    28861 aggccattgg cgagaaaaat caccagtaat ataagtacgt tatgccatac tgactggtaa
    28921 aagtagcgat agcggctact gtcattgcgg atgcgaaggc tttgttgtat ccacggtagc
    28981 aacaacattg ccatcagcag cagatagcag ccaaaatccc aaagcgcctt ttcagctctc
    29041 cagggggtca tgccatcagt ttgccacttc agcaagccgc tcattcccag cgtggcaata
    29101 aacaccagcg ccagccatcc ccaaaggcga ttctgtttaa aggagatcac ggagaagagt
    29161 aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa caatccaact gagatttttt
    29221 ccggcaagcc aggcgatcag caggtagcaa accagcccct gaagtaatcc ggtagcaatc
    29281 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    29341 aaggtgggtt aattatcgca tcgggcagta gtattttgct tttttcagaa aataatcaaa
    29401 aaaagttagc gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattcatga
    29461 atgcggttcc actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat
    29521 tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg
    29581 gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt
    29641 aacaccccca aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat
    29701 gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca
    29761 tgatatcgtt tacaacccaa gctacgatct gctgtatcag gaagagctgg acccgagcct
    29821 gaccggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat
    29881 cttcaccggt cgttcaccga aagataagta tatcgttcgt gacgatacca ctcgcgatac
    29941 tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacctctct ctccggaaac
    30001 ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt
    30061 cgacgccttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt
    30121 ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc
    30181 aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa
    30241 agaacagggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct
    30301 gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta
    30361 cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gcgagaaagg
    30421 cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc
    30481 gaaacgccgc ctgattggcg atgacgagca cggctgggat gatgacggtg tgtttaactt
    30541 cgaaggcggc tgctacgcga aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta
    30601 caacgctatc cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcacaat
    30661 cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat
    30721 cgataacatc gttaagccgg tttccaaagc gggccacgca actaaagtta ttttcctgac
    30781 agcagacgcc tttggtgtgc tgccgccggt ttctcgtctg actgccgatc aaacccagta
    30841 ccacttcctc tctggcttca ccgccaaact ggctggtaca gagcgcggca tcaccgaacc
    30901 gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc cgacccaata
    30961 cgcagaagtg ctggtgaaac gtatgcaggc agcgggtgcg caggcgtatc tggttaacac
    31021 cggctggaac ggcactggca aacgtatctc cattaaagat acccgcgcta ttatcgacgc
    31081 catcctcaac ggttcgctgg ataacgcaga aaccttcact ctgccgatgt ttaacctggc
    31141 gatcccaacc gaactgccgg gcgtagacac gaagatcctc gatccgcgta acacctacgc
    31201 ttctccggaa cagtggcagg aaaaagccga aacgctggcg aaactgttta tcgacaactt
    31261 cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaagctgta
    31321 atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc
    31381 ttcgcctccc gttttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcact
    31441 ggcagccagg cgcgaatgga aagcccgccc cgttcgctgg tgccaagctc cagcatcccg
    31501 ttatggttat ccacgatacg ctgcacaatc gccagcccta atcccgtgcc gctaatagtg
    31561 cgcgcactgt cgccacgaac aaacggctgg aacaggtgct tacgttgttc cggcgcaata
    31621 cccggaccgt catcttctac ctggaaccag gcgcgattcg gctccgtgcc gctgctgact
    31681 ttgatccagc cattgccgta acgggcggcg ttgaccacca tattcgctac cgcgcgtttg
    31741 atcgacagcg ggtgcatttt cacctcaatg ctgccggggt aaagcgcagt ttcaatttcc
    31801 cgctcatagc cactttcggc agcaataacc tcaccgagta ctgcgttgag atccgccatt
    31861 tccatcggca tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg
    31921 cactcttcga tatctttatt gatcgattct gccaggtagc catcctgctc gctcatcatc
    31981 tcggttgcca ggcgaatacg cgtcagcggc gtgcgcagat catggcttac ccccgccatc
    32041 agcagcgtgc ggtcatccgc cagttgctta acaccagccg ccatatgatt aaaggcgcgg
    32101 gtaacggaac gcacctccga agcgccatat tcacgcagcg gcggcggaat aatcccttta
    32161 cccacctgca aggctgcgtg ttcgagatcg accaacggtc ggttctggat acgaataaac
    32221 agccacgccc cgcctatcgc caatagcata atcgccaacg tataacggaa cagcggggag
    32281 aaatcgccct gatgaatttc ggttagcggc acgcgtaccc agatattggg agacaaccag
    32341 gttttcagcc agacgacagg cgaacttttg ttaacctcga cgcgaacttc cgtcggaccg
    32401 cccagttgct gcgccatctg atggcttaag aattcatagt gttgcgccca acgcagcccg
    32461 gcctcttcgg cggcttcgtt ggaatagaga gagatcccca gctcacggta gatctcccga
    32521 cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc
    32581 aacatacgca cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg
    32641 aagttcagca ccaccagata agtcgtcacc aggctgacaa acagcaaggt gacgatgagc
    32701 aataacgtac gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagaac
    32761 cgtccggtac aaagacgtag cccagacccc agacggtctg aatgtaacgc ggatgcgccg
    32821 gatcttcttc caccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg
    32881 cggaatattc acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg
    32941 gatggctgac cagtgccttc agtaccgcaa actcaccact ggtgagcggc atcggctcgt
    33001 cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg
    33061 cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg acgacgcagc accgcacgaa
    33121 tgcgggccag cagttcacgc gggttaaacg gttttggaat gtaatcgtca gcgccaatct
    33181 ccaggccaac gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg
    33241 ggttactctg gctacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatca
    33301 aatccagtac cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat
    33361 tagcgacgct tcgaacctgg aagccttgtt cggtgagata acgttccagc agcgcgcgca
    33421 ggcgcatgtc gtcatcaacc accagaatct tgtagttctc ttgcattgtt tgtactccca
    33481 aaggttcgca acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta
    33541 aatctggtat gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat
    33601 acaatttatt cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg
    33661 cgcattatca aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa
    33721 gggtatgaaa aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc
    33781 acaaagaaag tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag
    33841 tataataaaa agcgcctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg
    33901 gaaaatctca aaatcgtcga ttactccccg cagcaggaag gcaaagtctt ttttggtgcg
    33961 tgggtggaga ttgaaaacga cgatggtgtg actcaccgtt tccgtattgt cggctacgat
    34021 gaaatttttg gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg
    34081 aaaaaagaag tcggcgatct ggcggtggtg aatacccctg ccggggaagc gagctggtat
    34141 gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg
    34201 gcatttttgc cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact
    34261 caaaccatga tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg
    34321 gaacaggttg acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca
    34381 cgttatcgta aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg
    34441 cgtctgagct atctgcgcga gctggaagag agacgtcagg cgatcctcaa gtctatttcc
    34501 gagcaaggca aactcaccga tgatctggcg aatgccatca acgccaccct aagcaaaacc
    34561 gaactcgaag acctctacct gccctacaaa cctaaacgcc gcacccgtgg gcaaatcgcc
    34621 attgaagcag ggcttgagcc gttggccgat ctgctgtgga gcgatccgtc acacacgcca
    34681 gaagtcgccg ctgcacaata tattgatgcc gataaaggcg tggcagatac caaagccgcg
    34741 ctggacggcg cgcgctatat cctgatggaa cggtttgccg aagatgccgc gctgctggcg
    34801 aaagtgcgtg attatctgtg gaagaacgcg catctggtct caaccgtggt gagcggtaaa
    34861 gaagaggaag gggcaaaatt ccgcgactat ttcgatcatc acgaaccact gtcaacggtg
    34921 ccttctcacc gcgcactggc gatgttccgt ggtcgtaacg aaggcatact tcagctttcg
    34981 ctgaatgccg atccgcagtt cgaagagccg ccgaaagaga gctattgcga gcaaatcatc
    35041 atggaacacc ttggcctgcg cctgaacaat gccccggcgg atagctggcg caaaggtgtg
    35101 gtgagctgga cgtggcgcat caaggtgttg atgcatctgg aaactgaact gatgggcacc
    35161 gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg
    35221 ctgatggcgg cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtacc
    35281 ggggtaaaag tggcggtagt cgatgcaact ggtaaactgg tggcgaccga taccatttat
    35341 ccgcataccg ggcaggccgc aaaagcagcg atgaccgttg ctgcgctgtg tgaaaagcat
    35401 aacgttgaac tggtggcgat cggcaacggt acagcttccc gcgaaaccga acgtttctat
    35461 ctcgacgtgc agaagcagtt cccgaaagtg accgcgcaga aagtgatcgt cagcgaagct
    35521 ggcgcgtcgg tttactcggc ttccgagctg gcagcacagg aattcccgga tctcgacgtt
    35581 tcgctgcgtg gcgcggtgtc tatcgcccgt cgtttgcagg atccgctggc ggagctggtg
    35641 aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcag
    35701 ctggcacgca aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc
    35761 aacaccgctt ctgttcctct gttaacccgc gtggcgggcc tgacgcgcat gatggcgcaa
    35821 aacatcgtag cctggcgcga tgagaacggt cagttccaga accgtcagca actgttaaaa
    35881 gtgagccgtc tggggccgaa agccttcgag cagtgcgcgg gcttcttgcg cattaaccac
    35941 ggtgataacc cactggacgc ttctaccgtt cacccggaag cctatccggt ggtggaacgc
    36001 attctggcag caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt
    36061 aacctgaaag cgtctgactt tactgatgag aaattcggtg tgccgacggt aaccgacatc
    36121 atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt
    36181 gccgatggtg tcgagacaat gaacgacctg caaccgggta tgatcctcga aggtgcggtg
    36241 accaacgtca ccaactttgg cgcgtttgtc gatattggcg tgcatcagga cggcctggtt
    36301 cacatctctt cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc
    36361 gacattgtga aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgacc
    36421 atgcgtctgg acgagcagcc tggcgaaacc aacgcccgtc gcggcggcgg taatgaacgc
    36481 ccgcaaaaca accgcccggc agccaaaccg cgtgggcgtg aagtgcagcc tgccggtaac
    36541 agcgcgatga tggatgcgct ggcggcggca atgggcaaaa aacgttaaac gcccggactg
    36601 gcccctaaac ctgcgcgatt aggcgttcac gccacatctg gcaacgaacg ccttgcctga
    36661 cataaaagtg ccggagaata tctccggcct ttttattcca caagcaaacg cataatatat
    36721 cccgttaata tttatcactt cattaacaac tgaaacctta attaaacatt aaccagccct
    36781 aataattcgt catgcgaatt atattttcgc tgcgatataa gcttgagcca tatcaacatt
    36841 gagtcagatt attattcaaa ccaacattca cacacatttt aagtattgct gatagaaacc
    36901 attctcatta tcattgtact gttgattatt taatctctcc ttcgttggca aatcatctgg
    36961 tctcatgtcg ctgtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg
    37021 cacctatgca atacactcca gatactgcgt ggaaaattac tggcttttcc cgtgaaatca
    37081 gcccggcata tcgccaaaaa ctgctttctc ttggcatgtt acctggctcc tcttttaatg
    37141 tggtgcgcgt cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg
    37201 tattacgcaa aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg
    37261 ataacaacaa tgaaaaaatt aaccattggc ttaattggta atccaaattc tggcaagaca
    37321 acgttattta accagcttac tggctcacgt cagcgtgtag gtaactgggc tggcgttacc
    37381 gtcgaacgta aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg
    37441 cccggcacct attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc
    37501 tgtcactaca ttttgagtgg cgacgcagac ctgctgatta acgtggtgga tgcgtctaac
    37561 cttgagcgta acctgtacct gacgctgcaa ctgttggaac tcggcattcc ctgcatcgtg
    37621 gcgctgaaca tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctctg
    37681 tcggcgcgtc ttggctgccc ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa
    37741 gcgctcaagc tggcgattga tcgctataaa gctaacgaga atgtggaact ggtgcattac
    37801 gctcagccgc tgctcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg
    37861 ctgaaacaac gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc
    37921 tacgccggtg aagcgtcgca gcatctggat gccgccctcg cccgtctgcg taatgagatg
    37981 gacgatccgg cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat
    38041 gtggtaagca acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc
    38101 gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcttg
    38161 ctggctatca acatcggcgg ggcgttacag ccgctgtttg acgtcggctc cgtagcactc
    38221 tttgtgcatg gtattcagtg gattggctac acgctccact tcccggactg gctgactatc
    38281 ttcctcgccc aaggcctggg cggcggtatt aacaccgtgc tgccactggt gccgcagatc
    38341 ggcatgatgt acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcggcg
    38401 tttgtgatgg accgtctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtaccgctg
    38461 atcgttggtt tcggttgtaa cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg
    38521 cgtgaacgtc tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct
    38581 atcttcgcag tattcgcggc tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg
    38641 ctgtatatgc tgggtatcgt aatggcggtg ctgactggcc tgatgctcaa gtacaccatc
    38701 atgcgcggtg aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accccacgtt
    38761 aaaagcctga ttatccagac ctggcagcgt ctgaaaggct tcgttctgcg tgctggtaaa
    38821 gtgattatca tcgtcagcat tttcctgagc gccttcaaca gcttctcatt gagcgggaaa
    38881 atcgtcgata acatcaacga ctcggcgctg gcttccgtca gccgggtgat caccccggtc
    38941 ttcaagccga ttggcgtaca tgaagataac tggcaggcaa cggttggcct gtttacaggt
    39001 gccatggcga aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt
    39061 caggacgaag agttcaatcc ggcggaattt aacctcggtg aagagctgtt cagtgcggta
    39121 gatgaaacct ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt
    39181 gaagcaagca aaggcgacgg cgaaatgggt accggagcga tgggcgtgat ggatcagaaa
    39241 ttcggtagcg cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccatgt
    39301 atctcggtga tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatt
    39361 ctgtgggggc tgaatatcgc ttactcgctg gcaacattgt tctatcagat cgccagctac
    39421 agccagcatc caacctacag cctggtatgc attctggcgg ttattctgtt taacatcgtg
    39481 gttatcggtt tgctgcgtcg cgcgcgtagc cgggtggata tcgaactgct ggcaacgcgc
    39541 aaatcggtaa gcagttgctg tgcggccagt accactggcg attgccacta aggacacgac
    39601 aatggcttcg ctcattcaag tgcgtgattt actggcatta cggggccgta tggaagcggc
    39661 ccagattagc cagacactga acactccgca gccaatgatt aacgccatgc tgaaacagct
    39721 agaaagtatg ggaaaagcag tgcggattca ggaagaacct gacggctgtc tttccggcag
    39781 ttgtaaaagc tgcccggaag gaaaagcctg tctgcacgaa tggtgggcgt tgcgttaacc
    39841 ttgtttcact gcctgttttg aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc
    39901 ggctttccat agcagcgcga cgtaagttcc tctgaaagcg tctcgctggg atgaaaactc
    39961 gctaatgcac aggtgtggag tggcgcgtag agtcgcggca ttcaaataac aggtgaagga
    40021 acgccatgag caaaaagcag agttccaccc cacacgatgc gctgttcaaa ctctttttac
    40081 gccaaccgga gacggcacgc gagcatggcg ctgctggagt taattcaaaa acatattcgt
    40141 caacgcgatc tgataggact ggtagagcaa atggcctgct tattaagtag tggatacgca
    40201 aatgacagac aaatcaaagg gctgtttaat tacatactgc aaaccggcga cgcggtacgt
    40261 tttaacgatt ttatcgatgg tgtggccgaa cgttcaccaa aacacaagga gagtttaatg
    40321 actattgcgg aaagattgcg gcaggaaggg gaacaatcca aagccttgca tatagcaaaa
    40381 ataatgcttg aatccggagt ccctcttgca gacatcatgc gctttaccgg ggtgtcagaa
    40441 gaagagttgg ctgcggcgag ccagtaaagt tctgtcccgc catttcgaaa gccacctaca
    40501 ccctctgctt caacgccacc agcaggtgac aaaactcgtc cggatgtgaa ataaacggcg
    40561 catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta tccagcatcg
    40621 gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa aacggcatgg
    40681 gcacgttttg cagcggctga cggagatcga ccgttttcag gatttccagt ccgccattga
    40741 gtacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc cgcgcatcct
    40801 ggcgcgccgt ttcagtaccc atggtttgta acgccaggaa ccgctccact gtacgctgaa
    40861 aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc cccggccact
    40921 cgtcgcgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga acacgctcgg
    40981 gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct aaccaaatgg
    41041 ctttatctgg tgcctgtcgc agcacgactt cggccatatc agcaagtgat agcgcaccaa
    41101 atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctaa
    41161 gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc agcagcacaa
    41221 gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc tattgttctc
    41281 tttttactta caaggatgaa catatgctaa cagtaccggg attatgctgg ctatgccgaa
    41341 tgccgctggc gttaggtcat tgggggattt gttctgtctg ctctcgcgcc gcccgtacag
    41401 ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctctcatctt ccctgcggtc
    41461 gctgcctgca aaaaccgctg ccctggcaaa gactgctcac ggtcgccgac tatgcgccgc
    41521 cattaagtcc gctcattcac cagcttaagt tttcccggcg cagtgaaatc gccagcgccc
    41581 tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggc ttgcaactgc
    41641 ctgatcgcat catcagcgtt ccgttatggc agcgccgtca ctggcgtcgg ggatttaatc
    41701 agagcgattt gctgtgtcag ccgttatcac gctggctaca ctgccaatgg gatagcgaag
    41761 ccgtcacacg aacacgggcc accgcgaccc agcattttct taacgcccgg ctacgcaaac
    41821 acaacctgaa aaatgccttt cgccttgaat tgcctgtgca aggtcgccat atggtgattg
    41881 tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg cttttacgca
    41941 atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg
    42001 ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat ccgtatttcc
    42061 gatgctgcac aagcgcactt tgccaaactg ctggcaaacc aggaagaagg gacacaaatc
    42121 cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggcgtttc ttattgtccg
    42181 ccggacgctg tggaagccac cgacactgcc ctgaaatttg acctgctgac cgcgtatgtt
    42241 gatgagttaa gcgctcctta cctggaagat gcagagatcg attttgttac cgaccagttg
    42301 ggttcgcagc tgacgctgaa agcgcctaac gccaaaatgc gtaaagtggc tgacgatgcg
    42361 ccgctgatgg agcgcgtgga gtatatgctg caatcacaga tcaacccaca gcttgctggt
    42421 cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat tttgcaattt
    42481 ggcggtggct gtaacggttg ttccatggtc gacgtcaccc tgaaagaggg gatcgagaag
    42541 cagttgctga acgaattccc ggagctgaaa ggtgtacgcg atctcaccga acaccagcgc
    42601 ggcgagcact cttactacta agttctctcc ccttttgtcg attgcccgat gtgacgctaa
    42661 agcgtcttat cgggcctgta cgttgcacga gagtctgacg ttagcgcaga attatctcag
    42721 caaatccaat ctcttcgttt ccccacgata atatgaccaa cctctcataa tttaaattta
    42781 ccccgctctg gtgattctca agcacgtaat gttacccgta tcattctcat gggcaccaaa
    42841 cacactcata acatctgact acactaactc gttttagggg tcatcagccg tggtgccgta
    42901 acatctctgt tcccagttgg gacaaccagg attttccgtc agaaacctga cgttacccat
    42961 aacaaattaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg
    43021 ttattactcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg
    43081 cttgctgttg gattaatgca agggatgccg ctggataaag ttattggctc catcaaagct
    43141 ggtgtcggcg gaacgctcgg tagccttgcc ctgatcatgg gttttggtgc gatgctgggc
    43201 aaaatgctgg cagactgcgg tggtgcacaa cgtatcgcta ccacgctgat tgccaaattt
    43261 ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg
    43321 ttctatgaag tgggtttcgt gctgatgctg ccgctggtgt ttaccatcgc ggcttccgcg
    43381 aatattccac tgctgtatgt tggtgtacca atggcggctg cactgtctgt gacccacggc
    43441 ttcctgccac cgcatccggg tccgactgcg attgccacca ttttcaatgc cgatatgggt
    43501 aaaactctgc tgtacggtac tattctggca atcccgaccg tgattctcgc cggtccggtt
    43561 tacgctcgcg tgttgaaagg tatcgataag ccgatcccgg aaggtctgta cagcgcgaaa
    43621 accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc tctggtgccg
    43681 gtggttctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc
    43741 ctgccggtag cggagttcct cggtgacccg gtaatggcta cgctgattgc cgtgctgatc
    43801 gcgatgttca cctttggtct gaaccgtggc cgttcaatgg atcagattaa cgacacgctg
    43861 gtttcttcca tcaaaatcat tgcgatgatg ctgttgatta tcggtggtgg cggtgcgttc
    43921 aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat gcacgaaacc
    43981 aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat cgcgctgggt
    44041 tccgcaaccg tcgcggcaat cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg
    44101 ggtgttagcc cggagctgat ggttattgcg gttggttccg gtagtgtgat tttctctcac
    44161 gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat cggcgagacc
    44221 atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt aggctgtctg
    44281 ctgctgaata tggtgatttg ataatactga tggccggatg cggcgtgaac gccttatccg
    44341 gcctacgatt cggcattaat tagtaggccg gtaagacgcg tcaagcgtcg catccggcat
    44401 caaccgcact ctacttcttc ttcgctgccg ctctgcgccg tctgtccaaa tccttcagca
    44461 acttgttcac accatcatcg gcaaacatcg actcaagcgt tgcggaaagt ttacgtcgcc
    44521 agtttttata ctggtaactg gtgccgggaa tatttaccgg atctgccata tccagccagt
    44581 cttccggctg gagccctaac agggcgctgt tgctatcggc gatgtagcgc tgtaagccac
    44641 gattcagcgt cggtgtcatc gacatcaacg atgccttatg cccggcacgt tttggcaggc
    44701 aaccatattt atgcagagca tccagcagcc cttgcttcgc cagttcgcga tcctgataca
    44761 gaccgcgcag taccacttca tccggataca gccccagcgt tttgcccagc gttaaatccc
    44821 cactctccca gtagccgcgt agcgtaggca ggtcgtgtgt cgccgcaacc gccatcgact
    44881 gctctgggta cgctttcggt gcgcggaatg tcttctcgtg gtcgttttcg aaatagagca
    44941 ctttgtagga gtacacaccg ctgctgcgca gcttaccgac aatctctacc ggtacggtac
    45001 cgagatcttc accaatcacc atacagcgat gacgtttact ttcgagtgcc agaatcgaga
    45061 gcagatcatc caccggatag tgaacatacg cgccctgatc tgccgtctcg ccatacggta
    45121 tccaccacaa acgcagcatc gacatcacgt gatcaattcg taatgcgccg cagttttgca
    45181 tattggcacg caacagctcg ataaacggtt cataggcacg cgcggtgatg atatgcgggt
    45241 ccattggcgg caatccccag ttctgcccca acgggccgag gatatccggc ggcgcgccaa
    45301 ccgacgcttt caggcaatac agttcacggt cgcaccaggt ttctgcccca ccttctgcta
    45361 caccaacggc cagatcacga tacaagccaa tcggcatttc atagccctgg cttatctccc
    45421 agcaggcggc aaactggctg taagccagcc actgcaacca gagataaaaa tcgacgtcat
    45481 cacgatgttc ttcgcagaac tggcgcactt ctggcgaatc cacgttctga tacatctctg
    45541 gccatgctgg ccagccccag cgcatttcgt cctctttcac ttgctgggca tgtagcgcat
    45601 caaaggctgc ctgccagaac aggctgtcgc cctgctctgc aacaaactgg cgaaacgcgg
    45661 tcatctgctc atcatcacgt tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt
    45721 ttagggcggt aaccgtggag taatcaaccc agtcggcatc ccgcgcctgt tgcagcgtct
    45781 gttgcgtggt cggcaactgc caccaggcct gagcctcttc gctaagatgg aaatcttcaa
    45841 ctgcgttaac gtcgatataa atcacattca gccaacggcg agaagacggg ctgtacgggc
    45901 tggcactttc cggatttgcc agatagagcg catgaatcgg gttcagacca ataaacgacc
    45961 cgccacgttt tgccacatcc accagcatcg ctttgagatc gccaaaatcc ccaatacccc
    46021 agtttttttc cgatcgcagc gtataaagct gaacgcaggc accccacagc ttttgtttat
    46081 tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac aatcacccgg caatgcgcgc
    46141 gctggtcgtc ctgggtaagc gtcagcgtgt gataaccttc cggcagcttc gtcggtagat
    46201 tgaacgcttt gccccccgtc acatggcctt tgtactgctt tccttcttcg gtggtcagca
    46261 gccagctata ttcgccgctg ccctccacca ccatcggcat ttttttgccg ctggtataaa
    46321 ctatgacatt cgggactggc gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt
    46381 caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt
    46441 aattggggct aatccccgcc gccagcgcgg cattatccag acgtttgctt tccatcgaac
    46501 ttccttagcg ttttgcctgc cagatacgag cctgataatc gcgaatagag cgatccgagc
    46561 taaacatacc gcagcgggcg gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc
    46621 ggtacagcac atccacctgc ttttgtgcct ccacataagc ggcgaaatct gccatcacca
    46681 gatacggatc gccgccctgt ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat
    46741 cgccgtcgct gtatttaccg ctttccagct ctttcaatac tgcgtccagc accttatctt
    46801 tcttccgcca tttcaccggg tcgtagcctt tggccagaat tgccttcact tgttccacgg
    46861 tatgaccaaa aataaagata ttttcttcgc cgactttctc agcgatttca acgttcgcac
    46921 catccagcgt accgacagta agcgcaccat tgagcgccag tttcatattg ccggtaccgg
    46981 aagcttcttt acctgcagtc gaaatttgtt cggagatatc cgccgccggg atcagttttt
    47041 ccgccgccga aacacaataa tccggcaaga acaccacctt caacttgtcg ccaaccagcg
    47101 gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa gataatattt ttcgccaggt
    47161 agtagcccgg tgctgctttc gcgccgaaga ggaagacgcg cggtacgcgg tcagactgcg
    47221 ggttttcacg aatttctttg tacaacgcca gaatatgcag cagattcaga tgctggcgtt
    47281 tgtactcatg cagacgtttg atctgaatat cgaaaatcgc ctgtggattg atctcaatac
    47341 cggtacgaac tttcacaaac tctgccagac ggactttatt cgcctgcttg atctcgcgat
    47401 attgctgacg gaatttcgca tcatcagcga atttttccag attgatcagt tgatcgagat
    47461 cgttagccca ctcttttttc agtgatttat ccaacagagc cgccagtgcc gggttgcact
    47521 gtttgaccca gcgacgtggg gtaataccgt tggtgacatt atggaatttg ttcggccata
    47581 gctggtgata ttccgggaac agatctttca ccaccagatc cgagtgcagc gccgcaacac
    47641 cgttcaccgc gaaaccgcca accacacaca ggttcgccat atgcacttgt ttgtcgtgca
    47701 ccaccgccag tttggcccac actttttcat cgcccggcca ggttttctcc actagcgttt
    47761 taaagcgagt attaatttcg ttaataatct gcatgtggcg cggcagtaag cctttcacca
    47821 gtttcacatc ccagcgttcc agcgcttctg gcatcagggt atggttggtg taagcgaaag
    47881 ttttgctggt aatggcccag gcgtcatccc agctcatctg gtgctcatcg atcagcacgc
    47941 gcagcagttc tggaatcgcg attgttggat gggtatcgtt cagctgaata acttcgtaat
    48001 ccgccagttc gtgcaattta cgccccgcca gatgatggcg acgcaaaata tccgctaccg
    48061 agcaggcaca ctggaagtat tgctgcatca ggcgcagctt tttaccggca gtatggttgt
    48121 cgtttggata gagaactttg gtcagttttt ctgcgttgat gccctgctgt tcggcacgca
    48181 ggaaatcacc atcgttaaat ttagtcagat caaacggatg cgcatgcgtc gcctgccaca
    48241 gacgcagcgg ctgcgccacg ccattacgat agccgacaac ggggagatcc cacgcttgac
    48301 cggtaatggt aaattccggc tcccagcgtc cgtctttcgt cactttaccg ccaatcccta
    48361 cctgcacatc cagtgcttcg ttgtggcgga accacgggta gttaccgcga tgccagtcat
    48421 ccggcgcttc aacctgtttg ccatcgacaa aagactggcg gaacaaacca tattgatagt
    48481 tcagaccgta acccgtcgca gactgaccga cagttgccat tgagtcgaga aagcacgccg
    48541 ccagacgtcc cagaccaccg ttccccagcg ccgggtcgat ctcttcttcc aacaggtcag
    48601 tcaggttgat gtcataagcc ttcaacgaat cctgtacatc ctgataccag ccgagattca
    48661 acagattgtt gcccgtcagg cgaccaatca aaaactccat tgagatgtag ttaacatgcc
    48721 gctgattcgc caccggtttg gcaaatggct gagcacgcag catttcggcc agtgcttcgc
    48781 tcactgccag ccaccactga cgaggggtca tttcagccgc agaatttaag ccataacgct
    48841 gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag
    48901 gagttccact tttcttagat tttcaacaca acgttatcgc tagtttgcca ggctcgatgt
    48961 tgaccttcct catcctgcgg gggattaggc agggaggagt tgcggggatg agcaaggaaa
    49021 tgtgatctcg accacttaaa gctagtgcaa tccacaggat tagcagcaaa tcaatgccat
    49081 accgcgcaga aaatctgtat ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg
    49141 gaaggtaact ctataaatta agtaaaggag tgaaacagtt tcacaagtaa aatatcacgt
    49201 gtgctccatc tcattcttaa tagatttatt aagatcatct ttttagatgg cactttcatc
    49261 agaaatgaag aagaaaccct tgcttaaatg aaactgatga acataaggaa aataccgtac
    49321 aacccagtat tcacgctgga tcagcgtcgt tttaggtgag ttgttaataa acatttggaa
    49381 ttgtgacaca gtgcaaattc agacacataa aaaaacgtca tcgcttgcat tagaaaggtt
    49441 tctggccgac cttataacca ttaattacga agcgcaaaaa aaataatatt tcctcatttt
    49501 ccacagtgaa gtgattaact atgctgattc cgtcaaaatt aagtcgcccg gttcgactcg
    49561 accataccgt ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc
    49621 tggcgctgat cacaagtcct gcgggctacg gaaagaccac gctcatttcc cagtgggcgg
    49681 caggcaaaaa cgatatcggc tggtactcgc tggatgaagg tgataaccag caagagcgtt
    49741 tcgccagcta tctcattgcc gccgtgcaac aggcaaccaa cggtcactgc gcgatatgtg
    49801 agacgatggc gcaaaaacgg caatatgcca gcctgacgtc actcttcgcc cagcttttca
    49861 ttgagctggc ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca
    49921 ctaatcctgt gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca
    49981 cccttgtggt gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg
    50041 accagctgct ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaaacagt
    50101 tttttgattg ccgtctgtca tcgccgattg aagctgcaga aagcagtcgg atttgtgatg
    50161 atgtttccgg ttgggcgacg gcactgcagc taatcgccct ctccgcccgg cagaatactc
    50221 actcagccca taagtcggca cgccgtctgg cgggaatcaa tgccagccat ctttcggatt
    50281 atctggtcga tgaggtttta gataacgtcg atctcgcaac acgccatttt ctgttgaaaa
    50341 gcgccatttt gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg
    50401 ggcaaatgcg cctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata
    50461 ccggcgagtg gttttgctat cacccactgt ttggtaactt cctgcgccag cgctgccagt
    50521 gggaactggc ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc
    50581 agggatttcc cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc
    50641 gcgatattct gcttaatcac gcctggagtt tgttcaacca tagcgaactg tcgctgctgg
    50701 aagagtcgct taaggccctg ccgtgggaca gtttgctgga aaatccgcag ttggtgttat
    50761 tgcaggcgtg gctgatgcaa agccagcatc gctacggcga agttaacacc ctgctggccc
    50821 gcgctgaaca tgaaatcaag gacatcagag aaggcaccat gcacgcagaa tttaacgctc
    50881 tgcgcgccca ggtggcgatt aacgatggta atccggatga agcggaacgg ctggcaaaac
    50941 tggcactgga agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc
    51001 tgggtgaagt gttgcactgc aaaggcgaat tgacccgctc actggcgtta atgcagcaaa
    51061 ccgaacagat ggcacgccag cacgatgtct ggcactacgc tttgtggagt ttaatccagc
    51121 aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag
    51181 cgttccagct gatcaacgag cagcatctgg aacagctgcc aatgcatgag tttctggtgc
    51241 gcattcgtgc gcaactgtta tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc
    51301 gcagcgggat tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa
    51361 tgttgattca atgctcgctg gcccgtggtg atttagataa cgcccgtagc cagctgaacc
    51421 gtctggaaaa cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca
    51481 aagtccgggt gatttactgg caaatgacca gcgataaagc cgccgctgcc aactggttgc
    51541 gtcatacggc taaaccagag tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca
    51601 ttgcccgtgc acaaatcttg ctgggcgagt ttgaatctgc ggaaattgtc ctcgaagaac
    51661 tcaatgaaaa tgcccgcagt ctgcggttga tgagcgatct caaccgtaac ctgttgctgc
    51721 ttaatcaact gtactggcag gccggacgta aaagtgacgc tcagcgtgtg ttgctggacg
    51781 cattaaaact ggcgaatcgt accggattta tcagtcattt tgtcatcgaa ggcgaagcga
    51841 tggcgcaaca actgcgccag cttattcaac tcaatacgct gccggagctg gaacagcatc
    51901 gcgcacagcg tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg
    51961 atgagaattt cgttgaacgt ctgctaaatc atccggaagt gccagagctg atccgcacca
    52021 gcccgttgac ccaacgtgaa tggcaggtcc tggggctgat ttactccggt tacagcaatg
    52081 agcaaattgc aggcgagctg gaagtggcgg caaccaccat caaaacgcat atccgtaatc
    52141 tgtaccaaaa actcggtgtc gcccaccgtc aggccgcggt acaacacgcg cagaaactgc
    52201 tgaagatgat gggatatggg gtgtgagttt agccggataa tgcgtcggat ccggcttaca
    52261 tctttacatc attcaatgct aacccgcgtt acgccatctg tttctatcaa accaaaccgc
    52321 accggcaaga aacgctccac cacggcgata ttggtcagca gatgacatga gggatgggcg
    52381 accgtaaatt ctcccgcgcc cgccagcgcc atcggtagca ccagttggtc ggcgagatat
    52441 tcccccaccg ccgccgggct tgccaggtag cgtttcacct ctttcaccaa ctgtgccgcg
    52501 accacctcgg cactgacgcg cttttcaccg acgacaaaaa agcgttcggt gatattttca
    52561 ctttcgactt caagcgagac ggtattaccc ggcccctggt cgcgcggcag gttatgaata
    52621 ttctgttcat gcaaggaaaa actacccgcc agtgtagcga tttcacgctc agcaacatgg
    52681 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    52741 ggacgcatca cactggatac agtggaagat gagatcaacc gtctgcgcta taactggcag
    52801 gagagtcgcc cctccacgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    52861 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    52921 gctgccggac gtgaactgtt tgacatttcg cgccagggta aagccagcgt caatgacgcg
    52981 gatcggctac gcaaatacct ggcgcgtttt ggtctgacat gggaagcaat gcaggatcag
    53041 cacagctcca gttgaatatg atggtccttc agcacctgca tcacgctcgc aggcggcatc
    53101 gtgtcggtgt agacagcatc gaccatgctg atgctaccca tattgaccat tgcgttacgg
    53161 ccaaattttg agtggtcgac aaccagcata acgtggcgcg agttctcaat gatggcgcgt
    53221 ttggtgcgaa cttcgtggta atcgaactcc agtagcgagc cgtcgctatc gatgccgctt
    53281 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt tgcttcgcca
    53341 atgatcccgc catcgcggct gcgtaattcg ccaccggcaa gaatgatacg gaaatcctct
    53401 tttaccatca gcgtgttagc gacgttgaga ttgttggtca caatgcgtaa attgctgtga
    53461 ttaagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    53521 ccattgggaa tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    53581 gccttgcgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatga
    53641 tggcgcagga tcaggttttg ctctgccagc tcgttgagat cgcggcgaat agtctgcggg
    53701 ctaacggaga aatgctctac cagctcttcg gtgctgacat aaccctgctg tttaaccagt
    53761 tcgataatac cgttgtgacg ttgtgtttgt ttcattgata aatccctgga attattttcg
    53821 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat
    53881 atgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    53941 caacgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    54001 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    54061 cccgctgaat ttttgctgca catagccgct taacagggcg ctaatgagag taatgacaat
    54121 tagcttaccg ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag
    54181 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    54241 ctcaaatttc agcgtcggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    54301 gagaatttgc atggcgataa acaccaccac gcaggcgatc atcatcaccc aggttaccgg
    54361 acctgcgcgt tcacgcaagg cggcaaagaa aggataacgg cgataatgca ggccactgtc
    54421 ggtatgaccg gactgccaac tggcggcgag ataacgtgga tctgccgggt tttcgagaaa
    54481 acgagccagc tccgcccgca cgcgttcggc ctgggactca tccgccagcc agacatcgct
    54541 ttggttatgt tgttgaatcg tgaggataac accctgtgtc gccatgtaat caacaaacgc
    54601 ctgcgccact cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    54661 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgg
    54721 cgctgccagg cttcaaaacc gccatcaatg ctgtagacca catcgtagcc ctgttgcagc
    54781 agatactgcg ccgcgccttt gctgctattg ccgtgataac acatcaccat caccggtgtg
    54841 tcaaagtcgt tatcacgcat aaacgcgccc agtgtgtcgt tagttaaatg gaaagcctgg
    54901 gtcgcatgcc ccattgcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    54961 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    55021 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    55081 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    55141 tctttgctaa tatgttcgat aacgaacatt tatgaacttt aacgaaagtg aatgagggca
    55201 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    55261 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    55321 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    55381 aattccgact ggtcagcgag gcgctggctg aacgtgaagt gctactgaaa atggccccgc
    55441 atatcgcctt cccgatgcgt tttcgcctgc cgcatcgtcc gcatctacgc ccggcgtgga
    55501 tgattcgcat tggtctgttt atgtacgatc atctgggcaa gcgcaccagc ctgccgggat
    55561 caaccggttt gcgttttggc gcaaactcag tgttaaaacc ggaaattaag cgcggattcg
    55621 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    55681 tacgtaaagg cggtgaagtg cttactcgta ctcgtgccac gtctgctcgc cgcgaaaacg
    55741 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatataca tggcaggcgc
    55801 gcggcctggt taatgctacc ggcccgtggg tgaaacaatt tttcgacgac gggatgcatc
    55861 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc
    55921 atacccagaa gcaagcctac attctgcaaa acgaagataa acgtattgtc ttcgtgatcc
    55981 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggcgatccaa
    56041 aagcagtgaa gattgaagag agtgaaatca attacctgtt gaaagtgtat aacacgcact
    56101 ttaaaaagca gttaagccgg gacgatatcg tctggaccta ctccggcgtg cgtccgctat
    56161 gtgatgatga gtccgactcg ccgcaggcca ttacccgtga ttacaccctt gatatccatg
    56221 atgaaaacgg caaagccccg ctgctgtcgg tattcggcgg taagctgacc acctaccgca
    56281 aactggcgga gcacgcgctg gaaaaactga cgccatatta tcagggcatt ggtccggcgt
    56341 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg tgaccgtgac gattacgccg
    56401 cccgcctgcg ccgccgttac ccgttcctga ccgaatcgct ggcacgtcac tacgcgcgca
    56461 cttacggcag caacagcgaa ctgttgctcg gcaatgcagg ggcgataagc gacctcggtg
    56521 aagatttcgg tcatgagttc tacgaagcgg agctgaaata tctggtcgac cacgaatggg
    56581 tccgccgcgc cgacgatgcc ctgtggcgtc gcacgaaaca aggcatgtgg ctaaatgcgg
    56641 atcaacaatc tcgtgtgagt cagtggctga tggaatatac gcagcagagg ttgtcgctgg
    56701 cgtcgtaagg atgatgcccg gttgcgaagt gcaaccgggc tatttgaatt ccttagacta
    56761 ttcattgtaa ataattgaat aacggcccca gagctaaaat cgccaaactt aagcacgtta
    56821 cccaatcgcc ccacttcttc ctatggcttc tatttttgct acgccactgg caataatatc
    56881 ccaacgcaaa accaagctgg gcactaatcg ggccgacaaa atcagtccac ggctgcggcc
    56941 aaaaagggga aaaattcagg ggaagccaga caaatgtcag aaagaaattt cggatatttt
    57001 ccagcaacgc tgatttctct ggagaaatat aaagaatgaa attcgagaga attaaccaga
    57061 aatagaccgg agaaaaaacc agccagcaat gtagcctgtt ttttaattca ggaggcgttc
    57121 tgcgggagaa ttgccaaccg atgccaccca ttacactgaa aaaaagtgca tattcaacca
    57181 ataactcaag ttgtaattcc tgaagagaaa tacgcacaac caggagcgga aaggagaata
    57241 ttatttcagc cagaaaagtt aacgtgaata taagtacggc taaggcaata accgtcggcc
    57301 ataccaataa tggtacgata acaaaaccga aaattacagg gtaaatataa agtctgattt
    57361 tattcatctt taatgtcctg atgattcgga tgatgcgtca tacatggcac ataaacactc
    57421 atcaatcatc cactcgtctt cccaggcctg atactgtgtg ccctgttcgg actgcttaag
    57481 cggattctca atcgcctgcc gcgcgcgttc tttcaggatg gattgattat tactagccca
    57541 agtattagtt tgagtggtaa tggctttatc gatatcgtcc tgattatctc tgatataacc
    57601 gtcacgatac gtattcaata aacaggcaag agacgctgcc gaccaaaatc cgggaactga
    57661 aataatggca tcaagcaatg gcgaaagagc gatatcggca cggaaaaaca tttccagctt
    57721 gtactgtaat tcgacagcct catccccgga tacccaatca gtcatatact ggcaagcaaa
    57781 ttgctgcaat atttcccgtt catcctcacg ccagttttct ttactggcaa ggttgatacg
    57841 ccgtagagaa aatacttcgt gaatattgtc aaattgcccc tgcgcaacaa actccagtaa
    57901 acaaggcaga aaataacgaa cctcatcgct gttttgcgga tcaggtcctg gactactatt
    57961 ccaggcattg attaaggaat aaggaatcgc tcgcaacgat gtattccgta atgctttttg
    58021 ttcctgttga ctcagacaat attcacaaca aaccacaaat tgctgcggcg ccggataacg
    58081 acgaaaaata tgatatatct gctcaattaa tgcctgcatg gcaggagtgg ggtgcataat
    58141 taccccctcg cccgctgatc gtgcataaac ggctgataga taaaaagggc attcgccgcg
    58201 ttcaggaaaa tattaatgat atccatattg taatcctgct taatgcaacg ttttatccat
    58261 cggcacatag cccacatctt ccaccaaact ctgcccctgc ggcgttaaaa accactccac
    58321 caatttttgt gtctctgacg tcgtattttc ccgcgttacc attaaggcat cgacgatata
    58381 gggatatttg tcgttgcgaa tattttccgc agtcggtgca ataccgttaa tcgctagcag
    58441 ttttatattt ttatcggcat tcatttgtgt tgcgtaatag cggaaggtgt agcctattgc
    58501 gttttttgta ttacggtatt cggcaacaac gttaatcatt ccctccatca cgcttgccac
    58561 ttccgtttcc tgcggcgaga tcatgcggac atttttcatg acctgcgata gcatcaccgt
    58621 ctggctgcca gagtcttccg ggcgttgcca ggtctggatc tcctgatcgt caccgccaac
    58681 cgcgcgccag ttggtaattg cgccactgaa aatgtcacgc acctgttgtt cagtcaggga
    58741 attaaccgga ttatccgcat tgacgatgaa aacaaacgct tcacgggcaa atggcgtgta
    58801 cagcaaagtg acgcccgatt tctcagcgcg ttttttttgc ccatcggaag gttgcgccac
    58861 gaaaataata tcggcatcac cgttaacgat tttgttatat gcttcttgcg tacgagagtt
    58921 atccagataa tcccaaacgt gaaaatcctc tggtattaca cttaatgcat aaaatgcaga
    58981 ggcataaatg gggtacgccg ccgtagcgcc atcgagtcgc ggccagtttt gcgtaaattg
    59041 aatttgtggt ttaccacgca gtggcgtcag ttgattatta agtttatcgg gtcgccaggc
    59101 ccaggtatca atattttcac gcacaccgac accgggatta agctgtttat ataaataagc
    59161 ctgataccca gcgacaaaac caagtaagag aagtagaaac aggagtaagt tacggcaacg
    59221 tagcccggac gtgccggtta aaggatgcct tgtcggccag ccgtatccca gcgaaaaact
    59281 gatatgcgcg ccaacgggta taatgactat ccaccagtag tcgccactga ttaatgagag
    59341 taacaaaaca ccctgccagg aaagcataag gtttgccaac cagcccgatc ccatctggcg
    59401 aaattgataa tctgccagat gtagcgcgat tatccagcca accagggtta atagcgcggg
    59461 taagagaaaa aagcattgtc gccaggcggc tatttctctt ttttctggtg cgctatcacc
    59521 agcccacacg cgaccagaga aaaatatgat tatcgccccc agcgtcagca taaagacgtt
    59581 gagtagaaga taaattgttg gcgtaaagag taaattgcaa acctcaagaa tagcggcaag
    59641 cccggccccc agcatggcaa caaccgctgc caaaaattgc gccagtatgg ggatgccaaa
    59701 aaaagtcatt accagcgagg tcaaaatcca ttttccgctt tgcattattc tttccattct
    59761 tgctgaatgg cgaaattata ctccccgata accctcgccc cctctggaca cttttcctaa
    59821 ataaaggcga aaaaaacggg accttttggc cccgttctgt tttttagcga acttataatc
    59881 tcaccggatc aatatgccaa atataatcgg catactcttt gatcgtacgg tcagaagaga
    59941 agtaacccat attggcaatg ttcagcatcg cttttgcggt ccactcttcc tgacgctcgt
    60001 agagttcgtc aactttatcc tgacaatcga catagctgcg ataatccgcc agtacctggt
    60061 agtgatcgcc gaagttgatc agcgaatcga ccagatcgcg atagcgaccc ggatcttccg
    60121 gactgaatac accgctgccg atttgcgtca gcacctgatg cagctcctca tctttctcgt
    60181 agtattcacg cggtttgtag ccctgacggc gcaattcttc cacttcttcc gctgtgttac
    60241 caaagataaa gatattgtca gcaccgacat gatccagcat ctcgacattc gcaccgtcca
    60301 gcgtaccgat agtcagcgcg ccgttaagcg caaacttcat gttactggtg ccggaagctt
    60361 ccgtccccgc cagcgaaatc tgttcagaca gatcggccgc cggaatgatc aactgcgcca
    60421 ggctaacact gtagttcggg atgaacacga ctttcagctt gtcgccaatc tgcggatcgt
    60481 tgttgatcac tttcgctacg tcattgatca aatgaataat gtgcttcgcc atgtaatagg
    60541 ccgaagccgc cttaccgcca aaaatattca cgcgcggtac ccacttcgca tccgggtcgg
    60601 ccttgatgcg gttatagcgg gtgatcacgt gcaacacatt catcaattga cgtttgtatt
    60661 cgtgaatacg tttgatttgc acatcgaaca gcgctttcgg atttaccacc acattcagct
    60721 gctgggcgat atactccgcc agacgctttt tgttctccag cttcgcctga tgcaccgcgt
    60781 gattaaccat tgggaaatca cagtgttgtt gcagatcatt aagcaggcta aggtcggtgc
    60841 gccagttgcg gcccaggtgt tcgtccaaca cggctgaaag cgatgggttc gctaccgcca
    60901 gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa acgacccggg aagattttcg
    60961 caaagtcggc aaatagcgat tgcaccatca ggttagagtg cagttccgat acaccgttaa
    61021 ctttgtggct caccacaacc gccagccagg ccatacgcac acgacgacca ttggattcat
    61081 caatgatcga cgcccgtccc agcagatcgg tatcgttcgg atactgttcc tgcaaggttt
    61141 tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg acgcggcaga attttaccca
    61201 gcatatcgac tggccaggtt tccagcgcct cgctcattag cgtgtggttg gtgtaggaga
    61261 agacctgaca acacacttca aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca
    61321 gacgcatcat ctcaggaatc gacagcaccg gatgggtatc gttgaggtga atcgcaattt
    61381 tatccgccag gttatcgtag gttttatgca actgataatg gcggcttaaa atgtcctgaa
    61441 tggtcgagga aaccaggaag tattcctgac gcagtcgcag ctcacgcccg gagtaggtgg
    61501 agtcgtccgg atacagtacg cgagatacgt tctcggagtg gtttttatct tccactgccg
    61561 cgaagtagtc gccctggttg aatttaccga ggttaatttc actactggct tgggcactcc
    61621 acaaacgcaa cgtgttggtc gcgtcggtgt cgtaacctgg gattatctga tcgtaagcta
    61681 ctcccagaat ctcttcggtt tcaatccagc gcgttttttt accttcctgc tgaatgcgac
    61741 caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt
    61801 ccagccagta gtcaggcgac tctttctggc taccgttaac gatgttctgc ttgaacatac
    61861 cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc taacgtcgcc agagaatcga
    61921 ggaagcaagc cgccagacgg cccaggccac cgttaccgag gcccgggtca ttttcttcat
    61981 caatcagctc ttcgagattt aatcccatcg cttccagtgc gccctgtaca tcttcgtaaa
    62041 ttcctagcga caacatggcg ttggagagcg tacggccaat caaaaactcc atcgacaggt
    62101 agtaaacctg acgagtttct tgcgacaact gggcacggtt tgaacgtaac cagcgctcca
    62161 cgagacgatc gcgcacagca aataacgttg cgttcagcca ttcatgttta ttggcgacga
    62221 ccggatcctt tccaatcgta aacatcagct tgtaagcgat agagtgctta agagcttcta
    62281 cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat ataggcgttt cctgaaaact
    62341 atttcaagcg atagtaaagc tcacggtacg acttcgccgc gacctgccag ctaaaatcca
    62401 ttgccatagc ctgacgttgc acaaaccgcc acagtgaagg acgggaccac agtacgaaag
    62461 cacgtcgaat agcccgtaac agcgaccagg cattactatc ttcaaagaca aacccactgg
    62521 cgacgccatc tgcaaggttc tcaagagaac agtcagaaac cgtatcagca agcccaccgg
    62581 tgcgccgcac taacggcagc gtaccgtact tcaatccata aagttgcgtt aagccgcacg
    62641 gttcaaaacg gctgggcacc agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg
    62701 cttcgtgata gccaatctga acgcccacct ggccggggta ttccgctgcc gccgcaagga
    62761 aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca
    62821 gaagaccagg taaggcttcc agcaccaggt cgagaccttt ctggctggtc agacggctca
    62881 ccactgcaaa aagcggcact ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc
    62941 gcttattttc cgctttatct tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt
    63001 ccgtctctgg actccagatt ttctcgtcca cgccgttcag tacgccggaa agacgccctt
    63061 cacggtgacg ctgttgcaac agaccttcca taccgtaggc aaactgcggt tcggtgatct
    63121 cgcgagcgta ggttgggctg actgccgtaa tgtgatcggc atagtacaga ccggccttca
    63181 ggaaagagat ttgtccgttg aattccagcc catgaatatt aaagaatgac catggcaatt
    63241 ggatgtcatt catgtgatgt gcataaaaca tgccttgata ggccaggttg tgcacagtaa
    63301 acaccgactt cgccggacgc ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc
    63361 agtcgtgcgc atgcaccaca tcaggacgcc agaatgggtc aagcccgctg gccatttctg
    63421 ccccaaccca ccccagcagc gcaaaacgca atacgttgtc ggtataggca aataagttgg
    63481 tatcgtgata cgggcttccc ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc
    63541 caaccccgtt gtaatgaccg aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg
    63601 atactacctg cgcatcggtc acgccacggc gaatatcggg aaatgcaggc aacagtacgc
    63661 gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc cccaataaca tcagccagac
    63721 cgccggtttt aagcagcggg aacatctctg aacatacatg taaaacctgc attatcgctc
    63781 ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc agcacgatgc cttcttctga
    63841 acgatagaaa cgacgtgcat cttcctctgc gttttcacca atcaccatgc cttccggaat
    63901 aacacaagca cgatcgatga cgcagcgacg cagacggcac gagcgaccta cccatacttc
    63961 cggtaacaat acggcggaat caatgttgca gaatgaattc acgcgaacgc gcgagaacag
    64021 aacggactgc accaccaccg aaccggagat cacacaaccg ccggatacca gtgagttaag
    64081 ggtcatcccg tggctaccgg agcgatcctg cacgaatttc gctggcggta atgattcatt
    64141 gtaggtgcga attggccaat tgcgatcgta catatccagt tccggcacca cagaggccag
    64201 atcgaggttc gctttccagt aagcttccag cgtacccaca tcgcgccagt acggctcggc
    64261 atccgggtcg gattgtacgc aagagagcgg gaacgggtgc gcataggcca gaccggcttc
    64321 ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg gagttctcat cgcgatcgtc
    64381 ttcttccagc agttcataca gatagtcggc gtcaaagacg tagataccca tactcgccag
    64441 agatttgcct ggatcgttcg gcattgacgg cgggttagct ggtttttcaa cgaattcgat
    64501 aattttatcg ttctcatcaa ccgccataac gccaaacgcg gaggcttctt caatcggtac
    64561 tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg tggtcgataa gcatacgcga
    64621 gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc acgtattccg ctttataacg
    64681 gcggataatg tcgaggtttt gggtgaccgc atctgcggtg ccgcgatacc agttttcccc
    64741 tttcattctc tgctgtgctg gcagcagatc gacaaactcg ttcatttctt cattgaagaa
    64801 tgaccagccg cgctggatgt gctgcaccag agtgtgggac tggtactggg tgatcacgcc
    64861 catacgacgg atcccggagt tgatgcagtt agacagcgca aagtcgataa tgcggaactt
    64921 accgccgaag tgtacggccg gttttgctcg cttattggtt aaatccttca ggcgggtacc
    64981 acgtcctccc gccagtatca gggcaacaga tttcaatggc agctggcgtg ccaacattaa
    65041 gtgatcgttc ttctctaaac taaccatgac taactccttt tttatcatct ctggaacaca
    65101 cacaatccgt gtgcaggtcc ctgccagaca gccgtaatca ctgggttatc ctctccagcg
    65161 aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg
    65221 gcgttaattg cgatcaaaaa gcgatccgag agcagaattt gcagctgttt cggcccgttt
    65281 tgccactcat ccgtgcttaa aggttgagca tatcgattta gccaacggac attgccgtcg
    65341 ccttcttccc accagcgatt ctccatcaaa gcgggaatgc gcttgcgcag atggattaac
    65401 gcggcggtaa atgcggttaa accactgctt gcctgcgacc agtccaacca ggttaattgg
    65461 ttatcctgac agtaggcatt gttattgcca tgctggctgt gaccatgttc gtcaccggcc
    65521 agtaacatcg gcgtaccctg ggagagcaac aacgttgtta acagggcgtg aatgctgtcg
    65581 cgccgccgtt caaccagatc aagagtaccg cctaaccctt ctttaccatg attgttactg
    65641 taattgttgt tggtcccgtc gcgattttct tctccgtttg cttcattgtg tttatggttg
    65701 aagcaaacgc agtcgcgaag cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca
    65761 ctcggcagac gatcattacg tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac
    65821 gcccccagag gcaaatcata atgcagccag aaacgacggg ctgcatcgcg gaaatgatcg
    65881 ttccactcgg caaacagcgg cgggaaattt cccacctgat aaccaccagg agcgatatcc
    65941 cacggttcag caattaactt cacctgcgag agcaccgggc agttctggat agcggtaaac
    66001 aacggcgcat cctgacgaaa ctctggcgta cggcccatga ctgccgccag atcaaagcgg
    66061 aaaccatcga cgtggcaggt ttctacccaa taacgcaggc aggcgctggc ataatctacc
    66121 accgccggat gactcaaatt gagcgtgttg ccacaaccgg tccagttgtg ataatcgccg
    66181 tcttctctta tccaataata gctacggttg tcgatcccac gcagcgagaa taacgggccg
    66241 tcgaggtcca gttccgcact atggttgagc acgatatcaa gaatgacttc gatacccgct
    66301 ttatgcagtg ctttgattgc atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca
    66361 tacgccggat gcagcgcaaa catcgccacc gggttgtaac cccagtagtt actaagcccc
    66421 atacgttgca gacgtggttc actggcaaat tgtgccaccg gcagcagttc cagcgcggta
    66481 atgcccaact gtttcaaata gttgatcatc accggatgcc ccagggcttt ataagtgcca
    66541 cggatctcga ccgggatctc cgggtgcagg tacgttaatc ctttgacatg ggcttcataa
    66601 atgatggtgc tgccccacgg cgtgcgcggc ggggcatcat cttcccagtc atagtgatca
    66661 atcaccacta cgcatttcgg cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga
    66721 ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa tttgccgcgc gcaaggatca
    66781 atcaacaact tcgccgggtt aaagcgatgc ccctcggcag gttgccaggg gccatgaact
    66841 cgataaccat aacgcaaacc cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca
    66901 ctgtgccctg gcaagtcata gcgatgttcc tggccattgg catcaaagac acacagctct
    66961 acccgctcgg catgagcgga gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg
    67021 ccgaggggag tgggtttgcc aatggcgagt tgtgtcattc tgcctcccga accagccaga
    67081 tagtggccag aggtggtagc gtcaggctca gtgaatgttg acgaccgtgg ctggcaatct
    67141 catcgctgtg tactgcgccg ccattgcctg cattactgcc gtgatagtgc atggaatcgg
    67201 tattgaggat ttcacgccat ttacccggct ggttaatacc gaagcgataa tcatgacgcg
    67261 gcaccggtgt aaagttactg gcaacgatga tttcgttacc ctctttatcg cgacgcacaa
    67321 agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa
    67381 aatccagttc atgcattgct ttatggtggc ggtaggtgtg gttcagatcg cgcaccagac
    67441 gctggacacc gtggtgccag ttatcgccgc cttccaacag atgccagtcg aggctggcgt
    67501 catggttcca ctcgcggccc tgggcaaatt cgttccccat gaacagtagt ttcttgcccg
    67561 ggaatgccca catccagccg tagtaggcgc gcaggttagc gaatttctgc catgcgtcgc
    67621 ccggcatccg gtcgagaatc gattttttac cgtggaccac ttcatcatgc gacaacggca
    67681 ggacgaagtt ttcagtgtag ttgtagagca tcccgaaggt cagtttatcg tgatgatact
    67741 gacgataaat cgggtcgagc ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc
    67801 acttgtacca gaagcccaga ccgcccatat cctgtggacg agaaacgcca gggaaatcgg
    67861 tagactcctc cgccattgtc actgcaccgg aaacctgctc accaagaata cggttggtat
    67921 tacgcaagaa ttcaatcgct tcaagattct cgcgaccgcc aaattcgttc gggatccact
    67981 ccccctcttt acggctgtag tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg
    68041 catcaatacc aaaacgttca atccagtaaa gcgcgttacc gacaaggaag ttactgactt
    68101 cacggcgacc atagttgtag atcagcgtgt tccagtcctg atgatagcct tcgcgcggat
    68161 cgctgtgttc atacaagttc gtgccatcaa attcggcaag cgcaaagtca tcggtcggga
    68221 agtggcctgg cacccagtcg agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga
    68281 aataacggaa gtcgtcgcga gtaccaaaac ggcgggtcgg tgcatacagg ccggttggct
    68341 gataacccca actgccatcg aagggatgct cgttaatggg cagtagctcg agatgggtaa
    68401 agcccatcca tttagcataa ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga
    68461 aattgttgtc ggtgtggcga cgccaggagc ccaggtgaac ttcataaata gagattggcg
    68521 catcaaactg attcgctttt ttgcgctctt cagtctgcac aaccttttcc ggcagcccgc
    68581 aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa ggcataaggg tcggacttca
    68641 gacgcaagtt gccattggca tcaatcatct cgtatttata gagctgaccg ttatgcgccc
    68701 cagggataaa cagttcccag attccgctct ctttacgcag gcgcatcgga tgacggcgac
    68761 cgtcccagta gttgaattgc ccaaccaccg agacccgacg ggcgtttgga gcccagacag
    68821 aaaaacgcgt acctgtgacg ccatccatag tatctgcatg cgcgcctaag gtttcatacg
    68881 ggcgtaggtg agtaccttca gataatagcc aggcatccat ttcctggatt agcgggccaa
    68941 aacggtaagg atcatcaatc aggttttgct gaccatgcca gacaacagcc aactgatagc
    69001 ggaaaaaatt cttacgtcgc ggaatgacac cgctaaagaa tccccgtgag tcgagacact
    69061 ccagttttgc gagtttgcgc ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg
    69121 gtaaaagggc acggacttcc agtcccgcgg tggttttatg catccccagt acggaaaaag
    69181 gatccgcaaa atggcctgca attagcgcgt taatcacgtc tctatcgata cgatcggaca
    69241 tgcttgtctt cctgttttat tgtgtcaccc catccaactg aatttttgac ttctggttgt
    69301 gacatttttt tgacctgaac ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact
    69361 tcaggtaagg ctgtgaatac tcgtgtattc cgccaccctt aaagaatagc caatgctcta
    69421 tttaactccc ggtaaatcat gaaacatctg cgcttactcc tgtattacgc actagcaggg
    69481 gcggcatcgc gccccagatt taatgaataa agattacgcc agttgacgaa gcatccgacg
    69541 cagcggctcc gcggcccccc acagcagctg gtcgcccacg gtaaaggctg acaggaactc
    69601 tggtcccata ttcagcttac gcagacggcc taccggcgtg gtcagcgtgc cggtaacggc
    69661 agctggggtt agctcacgca tagtgatttc ccgatcgttt ggaacaactt tcgcccacgg
    69721 attgtgcgca gccagcagtt cttccacggt cggaatagac acatcttttt tcaatttaat
    69781 agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc acacataaac catctaccgg
    69841 aattacggaa gatgtgttga ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg
    69901 gctctgaccg ttatcaagct gtttgtcgat ccacggaatc aggctacccg ccagcggcac
    69961 gccaaagtta tccaccggca gctcaccgct acgggttaag gttgtgactt tgcgttcgat
    70021 atcgagaata gcagaggacg ggttcgcgag ttcatcagcc acatggccat acagatggcc
    70081 catctgggtt aataactcac gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt
    70141 tgcaacggac acccaatcaa caagatcatt ggcgaataaa ccacccagcg acatcaacat
    70201 caggcttacg gtacagttac cgccaacaaa agtcctgatg ccattattta atccgtcggt
    70261 aatgacgtcc tgattgacgg ggtcaagaat gatgatggcg tcatctttca tgcgcagaga
    70321 tgatgctgcg tcaatccagt aaccttgcca tccgctttca cgaagctttg gatagatttc
    70381 gttggtataa tcgccgccct gacaggtcac aatgatatcg agggccttta gcgcctccag
    70441 atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa gacggcgcag cctgaccaag
    70501 ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg
    70561 ttgcatgaga acggagccga ccataccgcg ccagccgata aaaccaacat ttttcataag
    70621 cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctcct ggcacatctt
    70681 tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc
    70741 agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat tatcagggaa
    70801 tttgagttat gaatgaaatc atttctgcaa cagttttatt gatcctgatt atggatccgc
    70861 tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa agacggcggg
    70921 caatcatggt gcgagagttg cttattgctc tccttgtgat gctggtgttc ctgtttgcgg
    70981 gcgagaaaat tctggcattt cttagcctgc gggcagaaac cgtctccatt tctggcggca
    71041 tcattctgtt tctgatcgcc attaagatga ttttccccag cgcttcagga aatagcagcg
    71101 ggcttccggc aggtgaagag ccatttatcg tgccgttggc aattccgtta gtcgccgggc
    71161 cgactattct cgccacgctg atgttgttgt ctcaccagta cccgaatcag atgggacatc
    71221 tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta cagtcttcgc
    71281 tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg atgggattga
    71341 ttctggtgat gatggcaact cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt
    71401 aaggttcaaa caggtgttgc acacaaccag acgcggcacc tgttttatta cctgaactga
    71461 taatgacctt cgcccaatcg gataaccctt ccctcttctt ctgcgcggtt cagccacaca
    71521 ctaatttgtt cgatggttaa accagtacaa gacgccagcc tttcaatact tatcgattcc
    71581 tttgcaagga tcgccagttc cgccacaaag agttgataaa aatcgacagg ggtatgcgcg
    71641 ggcattactg cgtaatttgc gtcatcttcg tgcagcgaaa acagttctgg ttgagcgtta
    71701 cggagtgctc tgtcatacat cagcccagga acgtctggca gtctgatcgt cacattctca
    71761 gcctgttcag cactccatga cgccccattt tctactaatc gtgaattggc tgattgcata
    71821 tcctgatttg gtttaacctg catgggcagc cactgatgtt ttaatgcctc catcgccccg
    71881 gttatcgttc caccggtcat tcccgcacgt acaaccattg cgctttcagc aaggcaataa
    71941 atatatttat tccgcgccat cgcattgccg acggtgaaac gtacctctgg gtaaaacggc
    72001 gaaatcaaca ccaggttgcc tgctataagc ccttcacgcc atttcactaa cgtactcgtt
    72061 tttaacaagc tatcagctaa tacgccaacg gcagttcccc cgacctccag agccgaggcc
    72121 attgcacatt catcaatacc tcgcgcacca ccagagataa cgcaaatccc ctgttgggcc
    72181 agtttcgcgg ccagttgttg ggtatagcgc aaatcgtcag tcggagcatc tcgcgagcca
    72241 acaatcgcca taccttccgc ttgcagtaat gctttattcc cgcagccaaa taaaacggga
    72301 ggtgcatccg ttcgcaatcg gtttttcaag cgaacgggat aatcggcatc tccgcgggtt
    72361 aagatccata aaccggcgcg ttgccactta tctacagcca acgccagact atgaccacgc
    72421 gccagcaagc caagaattcg ctcctgagaa atacgcggat cgctccagcc tgtaagtttc
    72481 tcttgcggtt gcggcaccag aagctcggcg ggactgatac gttggtgctt cagccataat
    72541 gcaaacttcc cccattcact attactaaga ggtttatact cactcgccgc cgcgcgagaa
    72601 aaatcgctgg ttagcaggag agttgcttga gcattggctg aaagattcat caatttttta
    72661 ccgaggtaga agcaagggca agaggataaa ccgtcgggca acctgcctgg cgtaacagtg
    72721 ctgtcagaac tgtcagcgtc catgcagaat ccacgatatc atcaaccagc aacgccggac
    72781 ctggcatcaa aggaggggta atcacaaacg ccccgtcgag attttgacac tggtgaaaac
    72841 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
    72901 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcctta
    72961 atgaaggaac gcagcatacc caggtgggtt caggatgcgg attccagcgt tggcgaacca
    73021 tttccgcaca tgcctccacc agctcatcac tgaagcgccc cgtttttttc tcctgggcta
    73081 cctgctttcc ccacccggaa tctccccaac gagaaaggat tcgccccgta gatccttgta
    73141 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agccccagat gccacctgtt
    73201 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagt
    73261 cgctatcaat gtcgggcgac aacagcggat gctgaagaca actgctacat ttaccacaac
    73321 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
    73381 tgatatagct ttcaacgtct gccaactcac tttttctctg attcatcaaa tggttaattc
    73441 gttcatgatc aggagaaaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
    73501 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgaa
    73561 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
    73621 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat tgatgaatag
    73681 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
    73741 gccccacctg ttggtagtaa ccgacaatag accccggcat ctgataatga atgacgaaac
    73801 ctaaatcagg tttatcaaat cccataccta atgccgtcgt cgcgaccagc actttgattt
    73861 tatttgccag cagtgcctgc tccagatact ccctggcagt attagaatct tccgcacctt
    73921 cacaagtcac gccgctgtaa taagcaaatg cactgatacc atttttcctt aaccactcgg
    73981 cgacaagttc agcatcgcga gtcgtcaggg tataaacaat tcccgatttg gaaaactgag
    74041 ggataaccgt tgctaaccat gccagacggg atgactgttc tcctaagact aaggcatcaa
    74101 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
    74161 ggatatcctc aacgacacgg ttattcgctg tcgcggttgt acccagaata ggggtattcg
    74221 caggcagttg gcgcaaaata tctaatatac gtcggtaatc cgggcggaaa tcgtggcccc
    74281 aatcagagat gcagtgcgcc tcatcgacca ccagtaaacc gatgcggtca gcgactggat
    74341 ataaaaccgt ttcaagaaaa tcctgattcg ccaaccgttc aggtgaaatc agcagacaat
    74401 ctacgccacc ttgcagcaac ttatcgctaa tgcgctgcca ttcttccctg tttgtagaat
    74461 tcaacgtttc agccgtaata cctaaccgtt cagcggcggc aacctggtta cgcatcaggg
    74521 caagcaaagg agagatgata attgtcgggc cagcgccgcg atcgcggaag attttactgg
    74581 cgatgaagta cacggcactt ttcccccagc cggtgcgctg tacaaccagt agttttcgcc
    74641 gctgattaac caccgcatct atagcttccc attgcccttc cctgaaagta gcgctggtgt
    74701 tacttaacat ccgctgtaac agcaattcag ctccgtgttt ctccatcatc ttctccctcg
    74761 tccgttaaca cagtgattag aacggataat gttccggtct ttttgcgaga gagatcaaaa
    74821 agatgaagac aggagaaaag agaaatgaat tactgcgagc aagctcgcag taatttgtcg
    74881 cagtaattaa ctcaacagct ggaacgcaat catcccaacg atggcaccga cagtgccgag
    74941 gatggtttcc atcatggtcc aggttttaag cgtttcggct tcggtcgcgc cggtaaattt
    75001 accgaacaac cagaaacccg cgtcgttaac gtggctgaca acaatcgaac caccggcgat
    75061 acaaatcgac agtgccgcca tttgcgcacc ggagtagttc agttgttcaa tgaccggcat
    75121 taccaatccc accgccgtta aacaggcaac ggtagcagaa ccctgaatga tgcgcactgc
    75181 agctgccagc acgaagcagg tgatggcaat cggcaggccc atgccggtta acgcttcgcc
    75241 cagtgccgga ccgacaccag agtcaaccag cacctgtttg aacacgccgc ccgcaccaat
    75301 caccagcaga atgatccccg ccggttgcag cgcgtgaccg caaatctcca tcactttgtc
    75361 ttttggcata ccctgacgca ttgccagacc gtaaatcgcc accaggcaag caaccagaat
    75421 cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa gcggtagaac cttctggcac
    75481 aaaacgcgcg gcaatggttt tcagccccac cagcaccagt ggcaacagga tcagcgacag
    75541 gctgaatccg aaggacggca ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg
    75601 aatatgcaac tccacgtagc ggctgatgaa attaccccac agcggcccgg caataatcat
    75661 tcccggaatt gccgcgcaca ggccaatcag gatcatccag ccaaagtcgg cgttcatctg
    75721 cgatgccagc agcattggcg ctggcccagg caccaggaac gcagccgctg ccgccacgcc
    75781 ggcgaataat gggattacca gcttcaccag gttcgtgccg gtgtggcgcg ccattgagaa
    75841 agcaacgcta atcagcagaa caatcgccac ttcaaagaac agcggtagcg cacaaaccag
    75901 ccccgcaagg ccgatggcat aatgcacgcg gctgtgaccg aaggatttga gcattttgac
    75961 ggcaatctga tcgaccgcgc cggtttcatg taagatctta ccaaacatgg ctcccagggc
    76021 gacaaccacc gccaggaagc cgagggtgcc tcccatccct ttttccatcg tcgctgcgat
    76081 tttatcgagc ggcataccgg aaaaaaggcc agcccccatg gacaccacca ttaaagccag
    76141 gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc agtaaaacag accctactgc
    76201 tgttaaaaca agcgttaatg tagtcactac ttatttgcct tttttaataa cctcaatggt
    76261 gcttgccaca acaccttcca gcggttgatc aatatccacc accagtacat cggtttcgtc
    76321 cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac atttgggttt taaagaaatg
    76381 gcctttgcgc gctttcaggc ggctttcaat cacatcaaaa tcgcctttca ggtagatgaa
    76441 agagagatta ggattacctt cacgcagcaa gtcgcgatag tgttttttca gtgcagaaca
    76501 gacgatcagc gatactttat tggtgcgctg catagcaaac gcggcgtcgt tcagcgcctg
    76561 caaccacggt ttgcgatcgt cgtcattcag cggttcgcca gacgccattt tttcgatatt
    76621 gcagcgcgga tggaggaaat cgccatcaag aaacgcggca tgaagttgat gcgccacttc
    76681 actggcgacc gcagatttgc cgctgcccga tacgcccatc aagacgtaaa tgtggtgatc
    76741 atggttagtc gtgctcaaag tggtgccccc acaatacaag aattaaaaat gttacgggta
    76801 actgttatcg gtaacattgt ccagccggac aatatcagaa gcaatatcca tccgtgcctt
    76861 aagtgtataa gtgtgagcta cttcaaattt gtaggcttaa atagatccgc ccggtgacaa
    76921 ggtgaaacct aaatctaaca ttttcggtgt cacagattcg ccacgaatac gcgccagcag
    76981 gcgctcagcg ccaatgctgc ccatccgctc acgcggcgtc agcacactcg ccagccgtgg
    77041 ctccatcacc tgaccaatgt catgaccgtg gaaaccggca atcgccatat cgtcaggaac
    77101 tttcaacccc agacgctgac attcaaacgc cgcgccgacc gccaggtcat cattagtaca
    77161 gaacacgcca tccagctgcg gatattcccg ccgcgcctgg cgaatcagtt caataccgga
    77221 agagtaagaa gaagattgct caaccatcac gctatacggc accaggcctg catccagcat
    77281 cgcctgttcg tatcccttct gtttgatgat agtacgttcg tcgagacgtg cgccgagata
    77341 ggcaatgtgg cgatgcccgc gagcaataat ggcggtggtc atctggcgtg ccgcttcaaa
    77401 gttatcaaaa ccgacggcga tatcgagaca cggcgactta ctgtccatca actccaccac
    77461 cggaatcccc gccacttcaa tcatctttaa ggtgcgtggc gtgtgggtac gttcagtgag
    77521 gatcaggccg tcgatattcc atgagagcat tgattcgagg cgttcttgct ccatttccgg
    77581 tttataaccg tagtgcgcca gcatggtttg ataaccgtgc gcgtcggtga cactttcgat
    77641 tccgcgtaat acttccgcga aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc
    77701 ccggctggtg gcgttagaga gtatatcggg cgcgcgattg ggaatataac ccagttcatc
    77761 aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc tgctccgggt tgcgtaaaaa
    77821 acggctgacc gtcattttgg tcacgcctac acggtcagcc acatcctgaa gtacgggtct
    77881 tttctttttc atcgtcctga aggtacaaaa gagatagatt ctcttagttt aacacggacg
    77941 catcacaacc ttcctgattc ctcaggaagg tttgtgagat aaatttagac tggcggtaaa
    78001 tcgaacagca ggatttcact gtcgctatca gcatggatgg aaatcgcctg ctcatcccag
    78061 attgccagac catcgctggt cgaagctttt acgccgttaa tggtgacatt gcctttcacc
    78121 acctggatcc agatgcggcg ttcagcggca atctgatgca ccgactgctc atctttcacc
    78181 agcgcccaac gatacagttc catatcctga tgcactttca atgaaccatc tcgcgcatcc
    78241 ggcgaaagca ccagttgttt gccctgcacg gcatcgaagc gacgctgttc ataacgcggc
    78301 gtaataccgt tttcttcagg catgatccag atctgataca gatgcagacg ctcggtgctg
    78361 cttgggttgt actctgagtg acgaataccc gtacccgcac tcataatctg gaactcgccc
    78421 gccggaacct gctctttatt gcccatgctg tcctggtgct caacagtacc ttccagcacg
    78481 taggtcaaaa tttccatatc tttatgcggg tgagtgccga agccctgccc tgcttcaata
    78541 acgtcgtcgt taatcacgcg cagcgcagag aagcccataa agttcggatc gtaatagttg
    78601 gcaaaagaga aagtatgcca ggagtccagc cagccatgat ttgcatgacc gcgttcattt
    78661 gctttgcgta agtagatcat ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg
    78721 gacccgatcc gcctgggatg atagagggtg aaaattgacc cctctgttca aaaaaattga
    78781 actattcgag gggccatcaa acttacctcg cgagactcac tgcggaggga gaagcttgct
    78841 caaaaccgcg ttcaaggatt tccagattcg taagaacttc agattccttg acgtaatttg
    78901 gcgcaccgtt ggtaatggtt tgatacaacg catcataaac gcgcccataa tcgcccatct
    78961 ctggcttcat ctcttctctt accgtcacgc cctcgtcatt gacatactcc agcacaccga
    79021 ccgaatcatc cgctgcgaat cctggttcgc ccggcataat attagccttc aggctggttt
    79081 cctgctggtc gataccgtat ttaataaacg aacctttctt accgtgaacg ataaatttcg
    79141 gataatcgat tttcaccaga tggctggttt tgacgatggc ttttagatcg ccataaaaca
    79201 gctgcgcttc aaaggtgtcg tccggattgg ctttattacg caggctgcgg atgtcataag
    79261 cgacgtgatc cgggcgaccg aacagagaaa taatctggtc catcgtatgc acaccaaggc
    79321 catagaacgc gccatcctgc ggcagccccg gtttggtttc tgccaccggg cggtaatagt
    79381 caaaatggct ttccacttcg acaatctctc ccagcttgcc gctttcaatc gcttttttcg
    79441 ctgtcaggaa gcaggaatca aagcgacgat tctgatacgg cgtaacgatc agccctttgc
    79501 ttttcgccag tgcaaacagc tctttcgcct gcgcaattgt cggagtgaac ggtttttcga
    79561 ccagcacatt tttcccggct tccagcgcgc gcttcgcgta ctcgaagtgg ctgtccgcgt
    79621 gggtgcagac gacaaccagc ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg
    79681 tgaaatggat atgggaataa atgggggcct gttcttccgg cttcgcatgg cggcgaaaaa
    79741 tatgcgcgac atgccagcta tccttgcggt taagtacata cggcagatgg taacgggtgg
    79801 tgcttttgcc gaagccaata aaggcgcagt taatgaccat gatttcgtcc tttttaaggt
    79861 ggttatttac accttagcgc aaagcggacg tggttcctac tggagtgcgc ataacgaaca
    79921 caagcacttc ccgaggataa attgagaacg aaagatcaaa aaaaaagcca gcacccggct
    79981 ggctaagtaa tactggaagc aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt
    80041 cttcctgatc gcgagacaat aataatcatt ctcattcgca cttgtccaac actttttgca
    80101 aaaaaatgca tttgactcgc atttgaaagt caatgatgtt gaaagggaca tttaccccaa
    80161 agaggacaaa ggaatgagtg agatagtaat acgccacgca gaaacacgcg attacgaggc
    80221 catcaggcag attcacgccc agccggaggt gtattacaac acgctacagg tgcctcatcc
    80281 ttcagaacaa atgtggctgg aacgactcac cgctcgccct ggcatcaagc agttagtcgc
    80341 ctgtattgat ggaatcgtcg taggccatct caccattgac gtgcaacaac gcccacgccg
    80401 cagtcatgtt gccgattttg gtatctgtgt cgactcccgt tggaagaacc gcggcgtcgc
    80461 cagcgccctg atgcgagaga tgattgaaat gtgcgacaac tggttgcggg tagatcgtat
    80521 cgagctaacg gtgttcgtcg ataacgcgcc agcaattaag gtctataaaa aatttggctt
    80581 tgagattgaa gggactggca agaagtacgc attacgaaac ggtgaatatg tcgatgcgta
    80641 ttatatggcg cgtgtgaaat agagaatttg caatctacgt ttgtcattta agccctcacc
    80701 taccgggaga gggcttaaga tattaatacc ccgccgttaa atcatccacc gagcgcgggt
    80761 cggatgcacc gtacagctca ccgtccggcc caaccataat gctttgtgta ctgcccatcg
    80821 cctctttcag cgccactttc tgaccttttg cttccagcag cttgatcgta tccgggctaa
    80881 accctttttc gacacgcagc tcgtccggca accactgatg gtggaaacgc ggcgcattgg
    80941 tcgcttcggc gacgttcatt ccataatcga tgctattcac caccatttgc agcacagtgg
    81001 tgataatccg gctaccgcct gggcttccgg tgaccagcca ggttttacca tccttgacca
    81061 caatggttgg tgacatcgac gacagcgggc gtttattcgg cccgacggcg ttggcatcgc
    81121 cgcccaccag cccgtaaacg ttcggtacgc ttggtttggc ggagaaatca tccatctggt
    81181 tattgagcaa aataccgctc tcgcccgcga caatgcccgt accgaaggtg gtgttcagtg
    81241 tataggtcac cgccacggcg ttaccgtctt tatccactac tgagtaatgg gtagtttgat
    81301 tactctcata aggcgcaagc ttgccaggac gaatttcgct ggatggcttc gctttattga
    81361 tatcgatttg atcggcaata gatttggcat aggctttatt ggtcagcgcc tgccacggta
    81421 ctttgacaaa atccgggtcg ccaagatatt ccgagcggtc ggcgtaggcg tatttctcag
    81481 cttctgccat gatttgcatc gcgtcggcgc tgccaaagcc gtacttctgc atatcgaagt
    81541 tttccagaat attgaggatt tgtacgatat gaataccgcc ggaagatggc ggtggcatgg
    81601 agtaaacctg atacccgcga taatcgccgc ttatcggtgt gcgttcgacc gctttatatg
    81661 ccgctaaatc ttctttgctg atcaagccac cgtttttctg catctcctgg gcaatctgtt
    81721 ccgctatcgt gcctttgtag aattcatccg ggccgttttc ggcaatcatc tccaggctct
    81781 ttgccaggtt cgcctgcacc agcttgtcgc cctttttcag cggttcgccc tctttccaga
    81841 agatagcttt gctgttttcg tggttcggca gcacttcact gccgtaggtt ttgagatcgt
    81901 cagccagcgc gtcgttaacg ataaaaccat cgcgtgccag tttaaacgcc ggttgcacca
    81961 ctttattcaa cggcatggtg ccgtatttat ccagcgccaa cgagaaaccc gctaccgtac
    82021 cgggtgtgcc ggaagccaga tgcgaagtga gtgatttttt gctgtccgtg ttgccctgat
    82081 catcgaggaa catatcgcgg gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg
    82141 tggtattgcc atttttcgag cggatcagca taaaaccacc gccgcccaga ttccctgcct
    82201 gaggatgcgt taccgccagc gcgtagccca ccgccacggc agcatcaacg gcattcccgc
    82261 cctccttgag gatatccact cccacctgag tggcagtaac atctacagac gccaccatcc
    82321 cttgtttcgc gcgtaccgga tggaagacat cttcctccac accataagaa acaggaggcg
    82381 cggctaccgt actaaaacaa cttcctgtga gcagagcagc aatggccacc cggcgtaaaa
    82441 acgtcggttt tatcatcgtt attctccaga gattaagggg caaccccagc taagcctggt
    82501 atataactct gaattaatca tcgttttgcc gggaagcgag taaacttaaa gaatatcttc
    82561 agaggaggat acgcgatgaa acgacttctg cttttgacgg cactcctgcc gtttgtcggc
    82621 tttgcacagc ccattaatac tctgaacaac cctaaccagc cgggatatca gatccccagc
    82681 cagcagcgga tgcaaactca aatgcagacg cagcaaattc agcaaaaagg gatgctgaac
    82741 cagcaactga aaacgcaaac gcagctgcaa caacagcatt tagaaaacca gataaacaat
    82801 aattctcagc gggtgttgca gtcacaaccg ggggagcgaa atcccgcccg gcagcaaatg
    82861 ctgcccaaca ccaacggcgg gatgttaaac agcaaccgta atccggatag ttcgttgaat
    82921 cagcagcata tgctgccgga gaggagaaac ggcgacatgc tgaatcagcc cagcacgccg
    82981 cagcctgaca ttccgttgaa aactattggg ccgtaaagtt cggaccaatc acgtcaatcg
    83041 catcggtaca gatgcaatcc acgccccagc gcagcaactc tgccgcgcgc tggggtttat
    83101 tgacggtata aaccagaatt cgcagtcctg catctttcaa ctgcatcact cgcgctttat
    83161 cgagcaactt ataattgaga tgaatagaga cgcagcccag ccgcgcggtc aattctcgcc
    83221 agtcgtcgcg ccattcatca agcaataaac cacgcggcag ttccggtgct gcctgttgag
    83281 cggcttctaa agcatcaatc tcaaacgatg acagcagcgg cggcgtcata ccggtccaca
    83341 gttcgcgtgc cgccaacgcc accattttgc ccgttaatgg tccagtgtca gtggtgggtt
    83401 tgatttcgat attcgccatc atcccgtgtt tgcgacagcg ttccgccacc tgcgaaagca
    83461 acggcagtgg ctcaccttta aacgctttgc tgtaccaact gcctgcatcc acgcgcagta
    83521 aatcctgcca gttcagttca cccgcgacgc cccagccgtt gctggtgcgt tcaagattgt
    83581 cgtcatggag caggaagatc tcgccatctt tcgataactt cgcgtcaaat tcgatcatct
    83641 tatgaccgta ttttgccccg acgtcgattg ctgccagggt gttctccggg gccagtttac
    83701 cgccgccacg atgggcgacg atgcggggat aaggccagtt actcatactc gttgtcctgt
    83761 ttcaccatca aaaagatgta gctgattttc cggcagatgc agccacagcg tgctgcctgc
    83821 cgtcgggcgc tcctgatgtg ccagtcgcac caccagcttc tgttcgcccc aatgtccgtg
    83881 cgccaggtta tctgcgccga ggatctccag cgtgtccatc accagcggca caccaccttc
    83941 tgcctgcgag cttagcgcta tatgttccgg gcgaatgccg agggtcattt tacgcccgac
    84001 atactgacgg tagccaccgt ttagcggcag cgcaataccg ccatccagtt cgaaatgcgt
    84061 accttcgtta ttcacacggc cagccagtag gttcatcgcc gggctgccga taaaactcgc
    84121 cacaaacagg ctggcgggct tttcgtacac ttcaactggc gtgccaatct gttcggcaac
    84181 gccgccgttc atcaccatta ctcgctgggc gagcgtcatc gcctcaacct gatcgtgggt
    84241 aacgtagagt gaagtcgttt tcaggcgacg gtgcagctgt tgcagttcaa gacgcatctg
    84301 cacacgcaac ttggcatcga ggttagagag cggctcatca aataggaaca ctgccggctc
    84361 acgcacaatc gcacgcccca tcgccacacg ctgacgctga ccgccagata gctcacgcgg
    84421 gcggcgctta agcaggccgt ccagctccag aatgcgcgcc gcttctttaa cacgttcggc
    84481 aatttgttgc ttgcccatgc cgcgaatttt cagcccccac gccatgttct cctctacgct
    84541 catatgcgga tagagcgcgt agttctggaa caccatcgca atcccgcgat ctttcggctc
    84601 catttcggtg acacgcttgc ggtcgatcca gatatcgccc gtcgttaccc gctccagtcc
    84661 ggccaccatt cgcaataacg tcgatttccc gcagccagac ggcccgacca tcacgataaa
    84721 ttcgccatcc gccacatcaa gggtcagcgg tttaatgacc tgggttttgc catcccagct
    84781 tttggttact gcctgtaatt tcagtcctgc catcttattt ctcactatcg accaggccgc
    84841 gcacgaaggc acgctgcatc actaaaacaa tcactactgg tgggatcagg gttaacaaca
    84901 tcgccgccat caccgagttc cattccgttg tgccttcgcc tgtggcgatc atccctttaa
    84961 tccctgccac ggtggtgccg agatcaacgt cggtaataat caacaacggc cacaaatact
    85021 ggttccagcc gtaaataaag gtgatcacaa acagcgccgc cagattggtt ttggacagcg
    85081 gaaaaacgat gtcgcaaaag aaacgcattg gcgacgcgcc gtcgatccgc gcggcttcca
    85141 ccagttcatc cggtagggtc ataaagaact ggcgaaacag aaacgtggcg gtcgccgagg
    85201 ccatcagcgg cagcgttaaa cctgcgtagc tgtcgagcat tttcaggttg gcgatgactt
    85261 ccaccgtcgg gaagatacgc acttcaaccg gcagcatcag ggtgataaaa atcatccaga
    85321 agaagaggtt acgtagcgga aaacgaaacc agacaatggc aaatgccgag agcatcgaga
    85381 cggtaatttt gccgagcgta atgctgaacg ccatcacaaa gctgttaagc aacatccgcc
    85441 agaacggcgc gctattcgtg cctaccccgt ttacccagat gttgtggatg ttttccagca
    85501 gatgtgtgcc gggaataagc gtcatcggcg cggcgtagac ttcttgttta tccagcgtcg
    85561 ccgcgacaaa cgccacgtac agcgggaaga ggatcaccgc gatcccgagg atcagcatgg
    85621 tatggctgaa tattgtcagc cacggacggt tctcaatcat tggtaacgta ccttgctttc
    85681 aacatagcgg aactgcacca ccgtcagcac gatgacgagg aacatcaaca ccaccgactg
    85741 cgcggcagac gaagccaaat ccagtccggt aaagccttca cggtagatct tataaatcag
    85801 cgtcgtggtg gcctgtactg gcccgccgga cgtggcggcg tcgatcaccg ggaaggtgtc
    85861 gaagaaggca tacaccaggt tcaccaccag caggaagaaa ctcaccggag cgataagcgg
    85921 cagcgcaatc ttaaagaagc ggcgaatcgg gccagcccca tcaatggctg cggcttcgat
    85981 caacgaacgg gggatggatt gcagcgcggc atagaagaac agaaagttgt agctgatttg
    86041 cttccacact gaggcaaaca ccaccaggaa catcgcctga ccgctgtttt gcgcgtggtt
    86101 ccagtcgtag ccgaactcag cgagaaaatg ggtgatcagc ccgcgaccgg ggttaaacag
    86161 aaagatccac aatacggcgg caacggcggg agccacggca tacggtagca acatcaaggt
    86221 ttgatagaaa cgactgccgc gcacgatgta ctccaccagc gctgcgaaga acagcgatac
    86281 cagcaaaccg ctgacggtga caaaggtgct gaattttatc gtcgtccaga aggcgtcgag
    86341 atagtagctg tcgtgaaaca gcgtgacgaa attatccagg ccgacaaact ggctggagaa
    86401 accaaacgga tcgacgcttt gtagcgagta ccacaacgct tcgcccgcag gccagataaa
    86461 aaagataacg gtgatgatga gctgcggcgc gaccagcaaa taaggtagcc agcgcgagcg
    86521 gaacaccgga cgggatgatg gcattactgt taattcctga actgtgccgg atgcgcttcg
    86581 cttatccggc ctacacgcga tgccaggtca gataagcgaa acgcatccga cattacactg
    86641 attaagactt cgtcgatttc tcaaagcggc gcagtaactg gttcccacgc tcaacggcag
    86701 tatccagcgc ctgctgtggt gtcttcttac cggtccacac gctctccagc tcttcatcca
    86761 caatcacgcg gatctgcggc atgttgccca gacgcaaacc tttggtgaac ggcaacggtg
    86821 gcttgttcag catctgacgc gtggcaatat ccgctcctgg gtttttctcg taaaagccct
    86881 gctcacgggt caggtcatac gccgctttag tgattggtag ataaccggtt ttctgatgcc
    86941 actcggcagc gttttctggt tttgcgagga agtcgaggaa cttcgccacg ccggtgtaag
    87001 tttctttatc tttaccctgc atcacccaca ggctggcccc gccgataatg gcgttttgcg
    87061 gcgcgtcttt cgcatcggcg tcgtaaggca tcatgcctac gccatagtta aatttggcgt
    87121 actcgcgaat gttggcaaga gaaccggaag aggcggtcgt catcgcgcaa tcaccgttat
    87181 agaacttctc ggtggattca tccttacgcc caacgtagct gaaatcgccc ttcttgttca
    87241 tctcttcgag catggcgatg tgtttcacct gctccggctt gttgaactca agcaccgcgt
    87301 ctgtgccgtc aaagccgttg tttttgctgg caaacggcag accgttccag gcgctaaagt
    87361 tttccagttg gatccagccc tgccagccgc tggcgtagcc gcacttcatg ccggaggctt
    87421 ttagttttgc ggcatagtcc gccagatcct gccaggtttt tggcggctgt tccgggtcta
    87481 atcctgcttt cttgaaggcg tctttgttgt aatagagaac gggggtcgaa ctgttgaacg
    87541 gctgggaaag taagtgccca gttttgctgt cggagtagta accggaaacc gtcggcacaa
    87601 actgcgactc atcgaattga atccttgcct ctttaaatac gtcatacacc ggtttaatgg
    87661 ctttcgacgc catcatggtg gcggtgccaa cttcataaac ctgcaaaata gccggagcgt
    87721 tgccggtacg aaatgcggca atccccgcgc ttaaattctg ttcgtaattg cctttatagg
    87781 tcggtacaat tttgtaatcc gggttttcgg cgttaaaacg ttgggccaga gaatccacct
    87841 ctttacccag ttccccttcc atagaatgcc agaacggaat ggtcgtcact gcctgcgcat
    87901 ttgccattaa agccagtccg agcgccagtg ctgaagctgt ataacgtaac ggtttcattg
    87961 attatctctc ttgttgtacc gaatgcgcga attcacgcgt tttatgctcg cggagtaaca
    88021 tgacatgctc gaattacaga aaaataacta ttttgttaca tttttaagat agtaaggtgt
    88081 cagaaagatg acaaggcggt gacggcgcgg gcgagggaaa atgggagatg gggcacggat
    88141 aagcggaaaa atagaaggtc tgaatcaaac tctacagatt actcatcgtt tcatgccgga
    88201 tgcggcgtaa acgccttatc cggcctacaa gatcgtgcaa attcaatata ttgcaactca
    88261 cccagtaggc ctgataagcg cagcgcatca ggcaatttta catttgtcac ctgtctcaaa
    88321 ggagtctttt gactccttat caatcaacgt gttattaccc gcctaaatat gcacttctca
    88381 ctgcttcatt cgccagcagc gcgtcgccgg tatcggagag cactacatga ccgttttcca
    88441 gcacgtagcc gcgatcagcc agctttagcg cctggttggc gttctgctcg acgaggaaga
    88501 tggtcatccc ctgctcgcgc agttgttcga tggtgtcgaa aatttgctgg atgataatcg
    88561 gtgccagccc gagcgatggc tcatcaagca gtagcaaacg cgggttactc atcaaagcgc
    88621 gaccaatcgc cagcatctgc tgttcaccgc cagacatagt gcccgctcgc tgaacacggc
    88681 gctcatgcag acgcggaaac agttcataga cccactttat gcgctcctgg aactggtcgc
    88741 gttcggcaaa aaagccgccc atcgccaggt tctcttccac cgtcatccgc gagaagacgc
    88801 gacgcccttc cgggacaatc gctaccgctt cgcgcatgat tttcgccgtc tgccagtcgg
    88861 taatgtcttt atcatcaaac acaattcgcc cgctggtggc gcgcggatcg ccgcataacg
    88921 tgccgagcaa ggtggttttc cccgccccgt tcgcgccaat gagggtgaca atctcgccct
    88981 gattgatatg caggctcacc tcatgcagcg cctggatttt gccgtagtgg gcgctgactt
    89041 tgtcaaagga caacatgact ttttccatct tatgcctcac ctaaataggc acggatcaca
    89101 tctgggttat tacggatctg ctccggtgta ccgtttgcca gcggcatccc ctgattgacc
    89161 acgtaaattc tgtctgaaat ccccatcacc agcttcatat cgtgttcaat caacaagata
    89221 gtggtgttgt gatggttacg cagttcggca atcagctcat ccagctcttt cgtctctttc
    89281 gggttaagac ctgctgccgg ttcgtcgagc attaaaatct ccggctgcgt caccatacag
    89341 cgggcaatct ccagacggcg ctggtcacca taggccaggt tactcgcctg acggttggca
    89401 tgttccagca aaccaatgcg ctcaagccag gtggcggcgc ggtcgagcgc ttcgctctgg
    89461 gcgcgacgga aagagggggt tttcaacagg ccggagaaca gcccagtttt cagttgctga
    89521 tgctgtgcca ccagcaggtt ttcaatcacc gtcatttcac ggaacagacg tacatgctgg
    89581 aaggtgcgca ccacgcccat gcgggcaatt tgctgccccg gtaaaccttc caggtgctga
    89641 tcgcgcagta aaatggtgcc gccggtgggt ttatagaatc cggtcaggca gttgaagacc
    89701 gtggtttttc cggcaccgtt agggccgatt aacgagacga tctcctgcgg gtacagttca
    89761 agattgacgt tgttcaccgc cagcaggccg ccgaagcgca tcatcaggcc gttaacagat
    89821 aataatggct gactcatgcc tgctctcctt ttgctgcgcc gtttttcagc ttgagttgcg
    89881 ggcgcgtcat gggcagcaag ccctgcggac gccagatcat catcagcacc atcaaaccac
    89941 cgagcattaa catgctgtat tcgttgaaat cacgcatcaa ctcgcgcgat accaccagca
    90001 aaacagccgc aagaatcact gcaaattgcg agcccatacc gccgagcacc actatcgcca
    90061 gcacaaacgc cgattcggca aaagtgaagg attcagggct gacaaagccc tggcgcgcag
    90121 caaacaacgt tccggcaaaa ccggcaaacg cagcgctgat ggtaaaggca gtcagcttga
    90181 tacgacgcgg gcttaagccc agcgaacgac aagcgatttc atcttcacgc aacgcttccc
    90241 acgcacgccc cagcggcatc cgcagcaggc ggttaatgac aaacaggctt aacaccacta
    90301 gcagcaacgc caccaggtag aggaagatga cgcgatcgga gggatcgtat ttcaggccaa
    90361 agaaattgct gaacgtgtcc cagccgcctt cgcgggcggt acggctgaac tcgagtccga
    90421 agagcgtcgg tttcggaatc tgactgatac cgttcggtcc gccggtaatt tcggtgttat
    90481 tgagcagcaa tatgcgcaca atttcgccga acccgagggt aacgatcgcc agatagtcac
    90541 cgcgcagacg cagcaccgga aaaccgagca ggaagcccgc cgccgccgcc attaacccgg
    90601 caatcggcag acaggtccag aagcccaggc cgtaatagtg attgagcagc gcaaaagtgt
    90661 aagcgccgat ggcgtaaaag ccgccgtatc ccagcaccag cagaccagaa agaccaacaa
    90721 ccacgttcag cccgagaccg agaataatgt agatcatggt cagagtggcg atatccaccg
    90781 tcccacgcga caccataaac ggccacgcca ctgcaagcac cagcagcgcc acgaggaaca
    90841 gtttctgctt caccgtggag ccatcaatcg cgggcagaat aaacttcggc ccggaaacgc
    90901 tttttagccc tttctggaaa gcaggccgca caagctggaa gaaaaagacc accgccgtgc
    90961 cgataaacac ccactgccaa cggatctctg aagccgtgtc gaccaccagt ttggtgccat
    91021 ccagctccag ttgcacgccc ataaagacgc ccgccagcac aaagaacata gcggcagaga
    91081 gcagcgccat tgcaatatgc atcggtttca tactttctct acctccgggc gacccagaat
    91141 accggtcggc atcaccagca gcaccaggat cagcagggcg aatgagacca catctttata
    91201 ttccgtactc agataggcag aagagagcgc ctccgcaatc cccaggatca gcccgccaat
    91261 catcgccccc ggaatgctgc caatcccacc gagcaccgca gcggtaaagg ctttcatccc
    91321 ggccataaag ccgatgtagg ggttaattac gccgtagaac tgaccgagca gcacgcccgc
    91381 cactgccgcc atcgccgcgc caatcacaaa ggtcagcgca atcacccggt cggtgttgat
    91441 gccaagcagg ctcgccattt tcagatcttc cgcgcaggca cggcaggcgc gccccatacg
    91501 ggaatagcga atgaaaatcg tcagcgccag catggcgagg aaagtaacaa tccatatcac
    91561 cgcctgcatg gtggtaatag acgcagagaa gttttcgcta tggcccacca cccactgacc
    91621 gttaaacagg ctgggcagcg ccacatcgcg cgaaccttcg gtcaggctga cgtagttttg
    91681 caggaagata gacatgccga ttgccgagat gagtgcaatc aggcgcttag agttgcgcac
    91741 cgggcgatag gctacccgtt cgatactcca gccgtaggcg ctggcaatga cgattgcgcc
    91801 gacgaatccc gccgccacca gcagccagcc ggtatcaatg cccagcatca tcagtgcggc
    91861 gatgatcata aaggagacgt agctgccaat catgtaaacc tcgccgtggg cgaagttgat
    91921 catgccgata atgccgtaaa ccatggtgta gccgatggct atcagcgcgt aggtactgcc
    91981 cagcgtgacg ccgttaaaca tctgctgcaa gaaatacaaa aactgctcag acataaggta
    92041 acctttctaa acccgcccgt ttttcacgga cggtgggatg atcacttggc tgccgtggat
    92101 gaaccgtcgg cgtgccactg gaagacacca aaatcgaatc ccttaagatc gcctttttca
    92161 tcccagttca gcggcccaat tacggtgttt gcaccgttag cttttaaatc tttcaccagc
    92221 gccagcggct catcgctgcc ggtacgttca agggcagttg ccagagattg caccgccgcg
    92281 taggtgatcc agacataggg cccggacgga tctttcttgt cggctttcag cgcatcaacg
    92341 atgccctggt ttgtcggatc ctggtcatag cgttttggca tggtgaccaa catgccttca
    92401 gcggcatcac cggcaatgtt cgacagtgac gcattaccga caccttccgg ccccataaac
    92461 tgggttttca ggccaacgga acgggcctgg cgcagcatct gccccatttc cgggtagtaa
    92521 ccgccgtagt aaacgaagtc gatgttttct tttttcaggc gggcgatcag cgcggagaaa
    92581 tctttctctc cagcggtaat gccgtcgaag aagacgacgt tggcgttagc cgctttcagc
    92641 ccgtcctgca ccgaacgtgc cagcccttcg ccatactgtt gtttgtcgtg aatgatggcg
    92701 atgcgctggg gcttcaccgt ctcaagaatg tattttgccg ccgttggccc ctgggaagag
    92761 tccagcccgg cagtacgcat gatgtattga taaccgcgtt gggtcagctc cggattggtc
    92821 gctcccggcg agatcatcag aataccttcg tcttcataga tatctgatgc aggttgggta
    92881 gaggaggaac acaagtgccc aataacgtat ttaatgccgt cgttaacgat tttgttggcg
    92941 accgcaacgg cttgtttcgg gtcgcaggcg tcgtcatatt ccacgccaac cagtttatcg
    93001 cccttaattc cccctttggc attaatgtct ttaattgcct gacgcgcgcc gttaaattcc
    93061 atatcgcccc actgggcaat cgggccggac atcgcgccga caacggcgac tttaatatcg
    93121 tcagccatag cggtgtgtga aattgccagt gcaatcatcc ctgcgatgat agttttcgca
    93181 ttccgtttca tagtcaaaaa tccccattcg tgatgttgtg ttgctttgtt cttatgtgtt
    93241 aacaaatcag actgttcttt tttatactgc actgttttta cctgtctgat taagggttta
    93301 gcgcagtatt ttgtgatgat agcgattaaa atccctattt ttc
//
LOCUS       DAPQZD010000003        79936 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPQZD010000003 DAPQZD010000000
VERSION     DAPQZD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.003"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(195..1052)
                     /gene="murI"
                     /locus_tag="RO454_000195"
     CDS             complement(195..1052)
                     /gene="murI"
                     /locus_tag="RO454_000195"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDY0932085.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
     gene            complement(997..2841)
                     /gene="btuB"
                     /locus_tag="RO454_000196"
     CDS             complement(997..2841)
                     /gene="btuB"
                     /locus_tag="RO454_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDY0932086.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2906..3096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3210..4310
                     /gene="trmA"
                     /locus_tag="RO454_000197"
     CDS             3210..4310
                     /gene="trmA"
                     /locus_tag="RO454_000197"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDY0932087.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4350..4709)
                     /locus_tag="RO454_000198"
     CDS             complement(4350..4709)
                     /locus_tag="RO454_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDY0932088.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(4709..5359)
                     /gene="fabR"
                     /locus_tag="RO454_000199"
     CDS             complement(4709..5359)
                     /gene="fabR"
                     /locus_tag="RO454_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDY0932089.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5463..6779)
                     /locus_tag="RO454_000200"
     CDS             complement(5463..6779)
                     /locus_tag="RO454_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0932090.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6822..7988)
                     /locus_tag="RO454_000201"
     CDS             complement(6822..7988)
                     /locus_tag="RO454_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDY0932091.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            8318..9718
                     /gene="sthA"
                     /locus_tag="RO454_000202"
     CDS             8318..9718
                     /gene="sthA"
                     /locus_tag="RO454_000202"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDY0932092.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(9701..10618)
                     /gene="oxyR"
                     /locus_tag="RO454_000203"
     CDS             complement(9701..10618)
                     /gene="oxyR"
                     /locus_tag="RO454_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDY0932093.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(10885..12258)
                     /gene="argH"
                     /locus_tag="RO454_000204"
     CDS             complement(10885..12258)
                     /gene="argH"
                     /locus_tag="RO454_000204"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDY0932094.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(12319..13095)
                     /gene="argB"
                     /locus_tag="RO454_000205"
     CDS             complement(12319..13095)
                     /gene="argB"
                     /locus_tag="RO454_000205"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDY0932095.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(13103..14107)
                     /gene="argC"
                     /locus_tag="RO454_000206"
     CDS             complement(13103..14107)
                     /gene="argC"
                     /locus_tag="RO454_000206"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDY0932096.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            14261..15412
                     /gene="argE"
                     /locus_tag="RO454_000207"
     CDS             14261..15412
                     /gene="argE"
                     /locus_tag="RO454_000207"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDY0932097.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            15714..18365
                     /gene="ppc"
                     /locus_tag="RO454_000208"
     CDS             15714..18365
                     /gene="ppc"
                     /locus_tag="RO454_000208"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDY0932098.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            18548..20281
                     /locus_tag="RO454_000209"
     CDS             18548..20281
                     /locus_tag="RO454_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDY0932099.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            20507..21358
                     /locus_tag="RO454_000210"
     CDS             20507..21358
                     /locus_tag="RO454_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0932100.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21345..21686)
                     /locus_tag="RO454_000211"
     CDS             complement(21345..21686)
                     /locus_tag="RO454_000211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDY0932101.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21688..22566)
                     /locus_tag="RO454_000212"
     CDS             complement(21688..22566)
                     /locus_tag="RO454_000212"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDY0932102.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(22532..24829)
                     /locus_tag="RO454_000213"
     CDS             complement(22532..24829)
                     /locus_tag="RO454_000213"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDY0932103.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(24880..25200)
                     /locus_tag="RO454_000214"
     CDS             complement(24880..25200)
                     /locus_tag="RO454_000214"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDY0932104.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(25215..26294)
                     /locus_tag="RO454_000215"
     CDS             complement(25215..26294)
                     /locus_tag="RO454_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDY0932105.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            26603..29104
                     /gene="ptsP"
                     /locus_tag="RO454_000216"
     CDS             26603..29104
                     /gene="ptsP"
                     /locus_tag="RO454_000216"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDY0932106.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            29116..29778
                     /gene="fsa"
                     /locus_tag="RO454_000217"
     CDS             29116..29778
                     /gene="fsa"
                     /locus_tag="RO454_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDY0932107.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            29789..30892
                     /gene="gldA"
                     /locus_tag="RO454_000218"
     CDS             29789..30892
                     /gene="gldA"
                     /locus_tag="RO454_000218"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDY0932108.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            31168..31785
                     /locus_tag="RO454_000219"
     CDS             31168..31785
                     /locus_tag="RO454_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDY0932109.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(31812..32717)
                     /gene="yijE"
                     /locus_tag="RO454_000220"
     CDS             complement(31812..32717)
                     /gene="yijE"
                     /locus_tag="RO454_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDY0932110.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(32811..34991)
                     /gene="katG"
                     /locus_tag="RO454_000221"
     CDS             complement(32811..34991)
                     /gene="katG"
                     /locus_tag="RO454_000221"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDY0932111.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(35320..36210)
                     /gene="metF"
                     /locus_tag="RO454_000222"
     CDS             complement(35320..36210)
                     /gene="metF"
                     /locus_tag="RO454_000222"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDY0932112.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(36592..38145)
                     /locus_tag="RO454_000223"
     CDS             complement(36592..38145)
                     /locus_tag="RO454_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDY0932113.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            38278..39408
                     /locus_tag="RO454_000224"
     CDS             38278..39408
                     /locus_tag="RO454_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDY0932114.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(39372..41804)
                     /gene="metL"
                     /locus_tag="RO454_000225"
     CDS             complement(39372..41804)
                     /gene="metL"
                     /locus_tag="RO454_000225"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDY0932115.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(41807..42967)
                     /gene="metB"
                     /locus_tag="RO454_000226"
     CDS             complement(41807..42967)
                     /gene="metB"
                     /locus_tag="RO454_000226"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDY0932116.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            43244..43561
                     /gene="metJ"
                     /locus_tag="RO454_000227"
     CDS             43244..43561
                     /gene="metJ"
                     /locus_tag="RO454_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDY0932117.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            43621..44229
                     /locus_tag="RO454_000228"
     CDS             43621..44229
                     /locus_tag="RO454_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDY0932118.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(44290..44502)
                     /gene="rpmE"
                     /locus_tag="RO454_000229"
     CDS             complement(44290..44502)
                     /gene="rpmE"
                     /locus_tag="RO454_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDY0932119.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            44705..46903
                     /gene="priA"
                     /locus_tag="RO454_000230"
     CDS             44705..46903
                     /gene="priA"
                     /locus_tag="RO454_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDY0932120.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            47059..48084
                     /gene="cytR"
                     /locus_tag="RO454_000231"
     CDS             47059..48084
                     /gene="cytR"
                     /locus_tag="RO454_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDY0932121.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            48176..49135
                     /gene="ftsN"
                     /locus_tag="RO454_000232"
     CDS             48176..49135
                     /gene="ftsN"
                     /locus_tag="RO454_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDY0932122.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            49228..49758
                     /gene="hslV"
                     /locus_tag="RO454_000233"
     CDS             49228..49758
                     /gene="hslV"
                     /locus_tag="RO454_000233"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDY0932123.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            49768..51099
                     /gene="hslU"
                     /locus_tag="RO454_000234"
     CDS             49768..51099
                     /gene="hslU"
                     /locus_tag="RO454_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDY0932124.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            51166..52092
                     /gene="menA"
                     /locus_tag="RO454_000235"
     CDS             51166..52092
                     /gene="menA"
                     /locus_tag="RO454_000235"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDY0932125.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            52185..52670
                     /gene="rraA"
                     /locus_tag="RO454_000236"
     CDS             52185..52670
                     /gene="rraA"
                     /locus_tag="RO454_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDY0932126.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(52755..53000)
                     /gene="zapB"
                     /locus_tag="RO454_000237"
     CDS             complement(52755..53000)
                     /gene="zapB"
                     /locus_tag="RO454_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDY0932127.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            53425..54270
                     /gene="glpF"
                     /locus_tag="RO454_000238"
     CDS             53425..54270
                     /gene="glpF"
                     /locus_tag="RO454_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDY0932128.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            54293..55801
                     /gene="glpK"
                     /locus_tag="RO454_000239"
     CDS             54293..55801
                     /gene="glpK"
                     /locus_tag="RO454_000239"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDY0932129.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            55972..56982
                     /gene="glpX"
                     /locus_tag="RO454_000240"
     CDS             55972..56982
                     /gene="glpX"
                     /locus_tag="RO454_000240"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDY0932130.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            57079..57825
                     /gene="fpr"
                     /locus_tag="RO454_000241"
     CDS             57079..57825
                     /gene="fpr"
                     /locus_tag="RO454_000241"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDY0932131.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(57830..58258)
                     /gene="uspD"
                     /locus_tag="RO454_000242"
     CDS             complement(57830..58258)
                     /gene="uspD"
                     /locus_tag="RO454_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDY0932132.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(58285..58584)
                     /locus_tag="RO454_000243"
     CDS             complement(58285..58584)
                     /locus_tag="RO454_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDY0932133.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(58796..59236)
                     /locus_tag="RO454_000244"
     CDS             complement(58796..59236)
                     /locus_tag="RO454_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDY0932134.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            59337..59936
                     /locus_tag="RO454_000245"
     CDS             59337..59936
                     /locus_tag="RO454_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDY0932135.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            60044..60811
                     /gene="tpiA"
                     /locus_tag="RO454_000246"
     CDS             60044..60811
                     /gene="tpiA"
                     /locus_tag="RO454_000246"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDY0932136.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(60866..61621)
                     /gene="cdh"
                     /locus_tag="RO454_000247"
     CDS             complement(60866..61621)
                     /gene="cdh"
                     /locus_tag="RO454_000247"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDY0932137.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(61728..62717)
                     /gene="sbp"
                     /locus_tag="RO454_000248"
     CDS             complement(61728..62717)
                     /gene="sbp"
                     /locus_tag="RO454_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDY0932138.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(63037..63999)
                     /gene="pfkA"
                     /locus_tag="RO454_000249"
     CDS             complement(63037..63999)
                     /gene="pfkA"
                     /locus_tag="RO454_000249"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDY0932139.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(64180..65082)
                     /gene="fieF"
                     /locus_tag="RO454_000250"
     CDS             complement(64180..65082)
                     /gene="fieF"
                     /locus_tag="RO454_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDY0932140.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(65231..65731)
                     /gene="cpxP"
                     /locus_tag="RO454_000251"
     CDS             complement(65231..65731)
                     /gene="cpxP"
                     /locus_tag="RO454_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDY0932141.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            65881..66579
                     /gene="cpxR"
                     /locus_tag="RO454_000252"
     CDS             65881..66579
                     /gene="cpxR"
                     /locus_tag="RO454_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDY0932142.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            66576..67949
                     /gene="cpxA"
                     /locus_tag="RO454_000253"
     CDS             66576..67949
                     /gene="cpxA"
                     /locus_tag="RO454_000253"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDY0932143.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(68120..68794)
                     /gene="yiiM"
                     /locus_tag="RO454_000254"
     CDS             complement(68120..68794)
                     /gene="yiiM"
                     /locus_tag="RO454_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDY0932144.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSVAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(68943..69926)
                     /gene="kdgT"
                     /locus_tag="RO454_000255"
     CDS             complement(68943..69926)
                     /gene="kdgT"
                     /locus_tag="RO454_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDY0932145.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(70186..70806)
                     /gene="sodA"
                     /locus_tag="RO454_000256"
     CDS             complement(70186..70806)
                     /gene="sodA"
                     /locus_tag="RO454_000256"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDY0932146.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQAYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            71091..72125
                     /gene="rhaT"
                     /locus_tag="RO454_000257"
     CDS             71091..72125
                     /gene="rhaT"
                     /locus_tag="RO454_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDY0932147.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(72122..72988)
                     /gene="rhaR"
                     /locus_tag="RO454_000258"
     CDS             complement(72122..72988)
                     /gene="rhaR"
                     /locus_tag="RO454_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDY0932148.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(73044..73880)
                     /gene="rhaS"
                     /locus_tag="RO454_000259"
     CDS             complement(73044..73880)
                     /gene="rhaS"
                     /locus_tag="RO454_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDY0932149.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHCVLQQVRQLVAQLEQQEEGNDLPSTASREILFMQLLLLLRKS
                     SLQESVENSASRLNLLLAWLEDHFADEVNWDAVAAQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            74168..75637
                     /gene="rhaB"
                     /locus_tag="RO454_000260"
     CDS             74168..75637
                     /gene="rhaB"
                     /locus_tag="RO454_000260"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDY0932150.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            75634..76893
                     /gene="rhaA"
                     /locus_tag="RO454_000261"
     CDS             75634..76893
                     /gene="rhaA"
                     /locus_tag="RO454_000261"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDY0932151.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLLDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            77160..77984
                     /gene="rhaD"
                     /locus_tag="RO454_000262"
     CDS             77160..77984
                     /gene="rhaD"
                     /locus_tag="RO454_000262"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDY0932152.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            77994..78308
                     /gene="rhaM"
                     /locus_tag="RO454_000263"
     CDS             77994..78308
                     /gene="rhaM"
                     /locus_tag="RO454_000263"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDY0932153.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(78349..79743)
                     /locus_tag="RO454_000264"
     CDS             complement(78349..79743)
                     /locus_tag="RO454_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDY0932154.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        19681 a        21409 c        20177 g        18669 t
ORIGIN      
        1 tacataatac gctttcccgc tacagagtca agcatttttt tgctttcctc tgttgagatt
       61 ctcaggagaa ccccgctgac ccggcggcgt gtttgccgtt gttccgtgtc agtggtggcg
      121 cattataggg agttattccg gcctgacaag aggaaattta aaataatttt ctgaccgcgc
      181 aacattcaac caaatcaatc taaaactgcc agtttttcga gcgtttcgaa gccgtaacgc
      241 tgtaaaacgg gcaataattg ttcagcttct ggcgtcattg ccatacaaaa tgcaatattc
      301 gcatcggcag attttgcatc cggggcttca tgctctaaca accaggccgt tcggcgagca
      361 atcgctgcgc cagaatccac cagccgggtt ccctctggca gcacttgtaa tagttcttct
      421 tgtagtagag ggaaatgggt gcaacccaat acaactgtat ctggcggctc tttcattctt
      481 aaccacgggc gtaggatacg ttttagcgca tccagagaaa catcttcgcc atgcagcttc
      541 gcttcagcca actcaaccat ctctgccgag cccagcattt ctatctggca ttcattagcg
      601 aaacgcgcga tcagctcatg agtataagaa cgtttaacag ttccgcgggt tgccagtaat
      661 ccgacaatgc catttgccgt cagacgtgca gcaggtttaa tcgccggcac gacaccaaca
      721 accgggaagt cgaacttttc gcgtaatgca ggaagtgaaa cggtactggc ggtgttgcaa
      781 gcgaccacag ccagcgcaag gggataacgt tcttgcactg cggtgacaat ttccaccact
      841 cgctcaacaa taaacacttc gcttttttcg ccatacggga aagcgacgtt atcgaaagca
      901 taaatgtaat ggagatccgg caagagatgc cggatctcgt catagaccga caacccaccg
      961 acgccggagt caaacaccag cacggtggga cgtggttcag aaggtgtagc tgccagacaa
     1021 ggtgtattcc cgtcctgcag tttggtagcc atagactgtc tcgtaatctt tgtcgaacag
     1081 gttggctatt ttaccacgaa ctgtcaggtg agaggtgacc ggatatgcaa cagcaagatc
     1141 ccacaagctc accccgccca ttttcactgt ttgatacgga taagaggagt aatccttatc
     1201 ataacgagaa cctaagtact gataagtaat cccccagtcg aagtcataca actgccagtc
     1261 gagctggtat ttcacctgct gtttagcacg gcgtaacaac ggcgtgtcag taatcgcatt
     1321 gcgcgcatca acataatcat aactcacagt atgcgtcagt ggtccggtat caaaattggc
     1381 ggtcgcctcg acacccttaa tccgcgcttt cccttcgttg taatatttca gggtgtgatc
     1441 atcataatcg atcaagtcac tgacatcgtt acgatatccg gaaatacgcc agttcacccc
     1501 agcagttaag ccttcaaacg cgccttccca ctgtttgctt ttctcagggt cgagattcgg
     1561 atttccgtag aaaccataca gttgtccaag atttggtgcc ttataagatg tcccgtagga
     1621 agcaataaag cgataacctt cgataaattc ccaaccggcg ctggtttgcc aggtgccatg
     1681 acgaccaaac tgtgagttat cgtcgctacg tgctgcgcct tcaaaggtaa aatcgccaac
     1741 ttgttgcagc ccggtcagat agatgccggt attacgttga tcatatccat cctcaacata
     1801 agctgtgccc ggtgccgtgc tctgcttctg ccagtcaaca cccgcaccaa tattaccgtg
     1861 gccaatgatg atgttgtttg cccactggac ggtgtactgt ttcatctcat cgagcgtcgc
     1921 ggatgaatca taacgaccat aatgcggatc gtagttgtaa tctttgctat ggctgtagct
     1981 ggtaatcagt tgtgatttaa tcagttcacc gttatagcgc agtccggcat cccaactttg
     2041 gctatagagt ttacgggtat cgaccagtgg cgagccggga gaataatatg cgtcataatt
     2101 ggtacgatta tcatagccat agccgcgcac aaagccgctc cagacatcag taaaattatg
     2161 ctccagcgcg ccataaagcg ttttacttaa aaaaccatcg ttatctggct gcgcttgcgt
     2221 tccggtatta ccataggcga caacatcata accatgagta tgggcataat cgcccaacag
     2281 cgttacccgt gtcttatccc ccagttgttg ctgtgtagag acatcatagt tctgataact
     2341 attgcttccc caccctgctg aaatttccgt tccggggtga tcgcgcgtcg tgatgatatt
     2401 cacgaccccg cctattgcat ctgaaccata aacagcggag cgcggcccac ggatatattc
     2461 aacacgctgg acaagcgcaa tagggaactg actaagatca gcagaaccac tcacccccgc
     2521 caaattcagg cggacgccat caattaacac caacacatga ctggcatttg taccgcgaat
     2581 aaaaatagat gagagctgac ctgaaccgcc gttttgggtg atatccacgc ccggaagacg
     2641 gcgcagcaca tcattgaccg aggtcgactg ccagcggtcg atatcctgac gcgtcacaac
     2701 agtagttggc gcaagcacag tgctgcgcgg ttgttcaaaa cggttagcag taacgacgag
     2761 agtatccggg ctggtatcct gtgcccaagc ggaaaatgcc gtgacggaac acgccgtcag
     2821 cagcgaagct tttttaatca ttgtaaagca tccacaatag aagaaggatg ccgcaggttt
     2881 catcaatatt acgcgatgat gagaaccaga tgcgacgttg gccggcaggt cttcgggctt
     2941 ggaggggtat ctaagatact aagagatgat gacttcccac cggatggcag tgtccgcata
     3001 acgcaatcat cgcacctttc cttaccgctg cgcgtcagct ccagattcgc actggattcc
     3061 ctattaactc acaggaccgg caagtggatg ctacaggttg taacaagtta ctgtccagac
     3121 gtagctcaca aataggaatt catcaagatc tggacatctg atgagcaatc cctacaatcg
     3181 ccgcgtactt taatttttca ggatacatca tgacccccga acaccttcca acagaacagt
     3241 atgaagcgca gttagccgaa aaagtggtac gtttgcaaag tatgatggca ccgttttctg
     3301 acctggttcc ggaagtgttt cgctcgccgg tcagtcatta tcggatgcgt gcggagttcc
     3361 gcatctggca cgatggcgat gacctgtacc acatcatttt cgatcaacaa accaaaagtc
     3421 gtatccgtgt ggatagcttc cccgccgcca gtgaacttat caaccagttg atgacggcga
     3481 tgattgcggg tgtgcgtaat aatcccattc tgcgccacaa gttgttccag attgattacc
     3541 tcactacgct aagcaatcaa gcggttgttt ccttgctgta ccataaaaag ctggatgatg
     3601 agtggtgtca gcaggcggaa gccctgcgcg atgcactgcg cgcgcagaat ctgaatgtgc
     3661 atctgattgg tcgggcaacg aaaaccaaaa tcgcgctgga tcaggattac atcgacgaac
     3721 gtctgccgat cgcggggaaa gagatgatct accgtcaggt agaaaacagc tttacccaac
     3781 cgaacgcggc gatgaatatt cagatgctgg aatgggcgct ggacgtaacc aaaggctcaa
     3841 aaggcgattt actggagcta tactgcggca acggtaactt ttcattagcg ctggcacgca
     3901 attttgatcg ggtattagcc accgaaatcg ccaagccgtc agtcgccgcc gcgcaataca
     3961 acattgccgc taaccatatt gataacgtac aaattattcg tatggcggca gaagaattta
     4021 ctcaggcgat gaatggtgtg cgcgagttta accgcctgca aggaatcgac ttaaagagtt
     4081 atcagtgcga aaccattttt gtcgacccac cgcgcagcgg tctggacagt gaaaccgaga
     4141 aaatggtgca ggcgtatcca cgtattctgt atatctcctg caatccggaa acgttatgca
     4201 agaatctgga aacattaagc cagacgcaca aggtcgaacg tctggctcta tttgatcagt
     4261 tcccctacac gcaccatatg gagtgcggcg tattactgac cgcgaagtaa aacccatgtc
     4321 ggatgcgcct gcatccggca tgatgctgat tactcagcaa cgtcctgctt acggttacgc
     4381 atcttcgcgc caatccagaa caccataatg acggacagta ctgccgggaa gaagttggaa
     4441 ccgatatccg gatattccgc acgaactacc gtgctgtaca gcagtacgcc gagaataaaa
     4501 caagcagctg ccagacccgg caagcctacc ggcatagtgc gattaagata acgttgatgc
     4561 aggcagtaaa ctgtcaacac cagggaaata atcgggaata cagaaaatgg tacaatggag
     4621 ctaaacagcg ctgcgaaagt accattaatc gataagccag ccactagcgc cagcagcagc
     4681 gtacctctat cttgatttgc ttgtttcatt actcgtcctt cacatttccc ggaataattg
     4741 cggttttctc ttgttcacgg cgataccagt aataagcccc tttcgaaatc attcgcagct
     4801 gcagtaccag tcgctcttct aattgccgac gttgctcgac gccgacatcc aacgcctcgg
     4861 cccccgcact gaagacaatc gtcaccattg cttcggcttg cgcttcagta aacgcacgcg
     4921 gcatatggtt ttcgagttcc agatagtccg caagttccgc aatgaagtgc tgaatttcac
     4981 gcgcaacggc ggcacgaaac gcagcggagg tgccggagcg ttcccgcaat aataaccgga
     5041 aggcgttagg attattaccg atgaactcca taaatgtgga gaccgaggtg cggatcacac
     5101 tcccgccttt ggcgatacgc tgacgcgcct gacgcatgag ttggcgtagc attaaaccgc
     5161 tctcatcaac catggtcaga cccagttcgt ctacgtcgcg gaaatgccga taaaaagagg
     5221 tgggagcaat gcctgcttct cgtgccactt cacgcaaact caggctggcg aagctgcgtt
     5281 cagcacttaa ttggctaaat gcggcttcca ccagcgaacg gcgggttttt tctttttgtt
     5341 gcgctcttac gcccatcacg atgtctgaat ccttgccaaa agtactgctg gcactatacc
     5401 agagaatgaa catactggat tgctccagtg ttgtgaatga acggtaacgc aataaaacat
     5461 atttactgca attctttggc cggttctttt acgtacaaca acgccagcat cgacagcaac
     5521 gtgcctccac ccagatacca cgccggagcc gtcagactat ccagcaacac gatggattgc
     5581 gtagcgaaat attgagcaaa accgccaaag attgccacac caatgctata aaccagcgca
     5641 aaacccaatg cccgaatccg tttcggcaac agttcagaga tcaacaacat cactggcaca
     5701 ccgccaagcg tggtaaagct gaccatcacg aaaaccaccg tcagcaacat gccagggctt
     5761 gggtaattca ccaacagcca gaatgacggc cagctacaga gaagcaccat cacacgggag
     5821 attaaaatca gctttttacg cccgaccgaa tcgcacaaca tccccacaag cagcgcaccc
     5881 acaaacgtaa caacgcccgc cagtaacatc gctgcatgag aataatgttg aggcatcgcc
     5941 agatacttcg ccgcccaggt gccgtaataa aagagggaga tataggttgc gaccgtgcta
     6001 ccaatagcga gtaaaacgcc attaacgata gtcgccttat gttgcaggag cagagaaaac
     6061 gcgctttcgc tggtagcagc ttttttattg cgcacaggct caggtacgtc attttccagg
     6121 ctcagacgca accagcaacc gataggtgct aataacacac caataaaaaa cggcacacgc
     6181 cagccccatt ctgccattac ggtttctgaa cctgtggcga acggtagcca tgcgcttaaa
     6241 cctaaagcga caacaccgcc aaaagttgtt gccaggcctt gagtcgccag tgaccaactg
     6301 ctgtaaaagc cacgacgatt tgccggagct gactccacca gtagcgacat tgaagccccg
     6361 acttcaccac ctgccgcaac gccctgaatc aaacgcgcca gcaccagcgt tgcggttccc
     6421 caatagcccg ccgtttcata cgtcggtgcg atgccgatca tcagcgtacc gaggctcatc
     6481 agagcaatag tgaacaccat cgccggttta cgcccgaagc gatcggccca ggcaccaacg
     6541 ataatcccac cgagcggacg cataaaaaag ctcacaccaa acgttgcaaa tgccagtaac
     6601 agcgcgacgt tatgatcggc actgggtggg aagaaaagcg tactgatata gaccacaaaa
     6661 aagttgtaaa cgccaaaatc aaaaaattcg agcgcattgc caaacgccga ggcaaagatc
     6721 acccgcctgc cgctgggctt tttcttcaca ctcacggttt catttacgat cgccgtcatt
     6781 ccatgttcct tgccaaaaag tgttgttgtg tgttgtttta tttattgcag ccaggcttca
     6841 accagtgcgc cccagtagct gcttgccggg accaggcttg catcgttaaa gtcgtaaccg
     6901 gggttatgca ccatgcagga accttccccg acatcgccat taccgaataa caggaaaccg
     6961 cctttgggtt gggcctccag catgaaagag aaatcttcac tgcccatcag gggtttgatg
     7021 ccaaactcag cccgatcttc accaaatgtt ttgcacgcca cctgccaggc gaagcgcgcc
     7081 atctcctcgt catttactaa taccggtgta ccgttaacat gcgtaacctc agccgtagcg
     7141 ccaaagctgg cagcctgggt ttgtgcaaga gcaggaatac gggtcagaag aagttgtcgg
     7201 gtatcgcggc taagagagcg gacactgatt ttcatctcag cactgtcagg aatgacgtta
     7261 gccgcctcac ccgcaacaat gctgccgacg gtaattaccg ccgcttccag cggatcgaca
     7321 ttgcgcgaca caatgctttg taatgcggtg gtgatatgtg cggcaaccag cactggatcg
     7381 atggctttgt gcgggatcgc accgtgtccg ccacaaccac gaaccgtaat atggaactga
     7441 tccattgacg ccatcagcgc cccaggctgg aagaaaaact tgccgaccgg caaacccggc
     7501 atgttgtgca tcccgaaaat gacatcgcag gggaaacgat caaaaagccc ctctttaacc
     7561 atgatctcac cgccgttaat catctcttcc gctggctgga agataaggcg taacgtgccg
     7621 ttaaaacggc gggtttcggc aaaataacgc gcggccccca gcagtatggt ggtatgcccg
     7681 tcatgcccgc aggcgtgcat taaaccagga tgtttactgg cccagggctt gccactattt
     7741 tcataaatcg gcaacgcatc catatccgcc cgcaggccaa tacttttctc tccgtcgccc
     7801 acttttaacg tggctaccac gccggttgtc gccagccctt gctcaacctc atagccccac
     7861 tggcgcagct tttccgcaac caaagcactg gttttaaatt cttgtaatcc cagctccggg
     7921 ttttcgtgga tctgatggcg gatctcgcga agttcttcct caaacccacg aatatagtgt
     7981 tcgatcatcg gctattcctt tattcactta taaacaggct aattaacgga ttttattgat
     8041 ggtataaata caaataaccc atcaatataa aatatttata catttgtttt ataagaagat
     8101 ttcataaaga caataacaaa aaagcacgct tcggcaagat aaaactatca gccataagaa
     8161 aaagcgatta atagtgaata aataaaataa gaaggaggaa gacaaagcaa aggccgctca
     8221 cgatatagcc agataaatga cggggatcaa ttggcttacc cgcgataaaa tgttaccatt
     8281 ctgttgcttt tatgtataag aacaggtaag ccctaccatg ccacattcct acgattacga
     8341 tgccatagta ataggttccg gccccggcgg cgaaggcgct gcgatgggcc tggttaagca
     8401 aggtgcgcgc gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt gcacccactg
     8461 gggcaccatc ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag aattcaatca
     8521 aaacccacta tacagcgacc attcccgact gctccgctct tcttttgccg atatccttaa
     8581 ccatgccgat aacgtgatta atcaacaaac gcgcatgcgt cagggatttt acgaacgtaa
     8641 tcactgtgaa atattgcagg ggaacgctcg ctttgttgat gagcatacgt tggcgctgga
     8701 ttgcccggac ggcagcgtcg aaacactaac tgctgaaaaa tttgttattg cctgcggttc
     8761 tcgtccatat catccaacag atgttgattt cacccatcca cgcatttatg acagcgactc
     8821 aattcttagc atgcaccacg aaccgcgcca tgtacttatt tacggtgctg gagtgatcgg
     8881 ctgtgaatat gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga tcaacacccg
     8941 cgatcgcctg ctggcatttc tcgatcaaga gatgtcagat tctctctcct atcacttctg
     9001 gaacagtggc gtagtgattc gtcacaacga agagtacgag aagatcgaag gctgtgatga
     9061 cggtgtgatc atgcatctga agtcgggtaa aaaactgaaa gctgactgtc tgctctatgc
     9121 caacggtcgc accggtaata ccgattcgct ggcgttacag aacattgggc tggaaactga
     9181 cagtcgcgga cagctgaagg tcaacagcat gtatcagacc gcacagccgc acgtttacgc
     9241 ggtgggcgac gtgattggtt atccgagcct ggcgtcggca gcctatgacc aggggcgcat
     9301 tgccgcgcag gcgctggtga aaggtgaagc caccgcacat ctgattgaag atatccctac
     9361 cggcatttac accatcccgg aaatcagctc tgtaggcaaa accgaacagc agctgaccgc
     9421 gatgaaagtg ccatatgaag taggccgcgc ccagtttaaa catctggcac gtgcacaaat
     9481 cgtcggcatg aacgtgggca cgctgaaaat tttgttccat cgggaaacaa aagagattct
     9541 gggtattcac tgctttggcg agcgcgctgc cgaaattatt catatcggtc aggcgattat
     9601 ggaacagaaa ggtggcggca acactattga gtacttcgtc aacaccacct ttaactaccc
     9661 gacgatggcg gaagcctatc gggtagctgc gctaaatggc ttaaaccgcc tgttttaaca
     9721 ctttatcgaa atggccatcc attcttgcgc ggatggcctc tgccagctgc tcatagcggc
     9781 tgcgcagcgg tgagccagga cgataaacca ggccaatagt gcggcgtggt tccggcttaa
     9841 tgcacggcag ataaacaacc ccatcgcgtt tgcgttccgg cggcacagcc agcgcaggca
     9901 gtaaagtgat cccgctacct gccgccacca tgttgcgcag agtttccagg ctggtcgcgc
     9961 ggaagtgtgt atcttcatcc gccccggctt caaaacagaa acccattgcc tgatcgcgca
    10021 aacagtgacc atcttccagc atcagcagtt tttcccctgc cagatcggcc atcggtacgc
    10081 attcgcggtt cgcccacggg tggtcttcat agatagccag caacattggc tcatcaaaca
    10141 acggcacttc aatgaatgct tcgctctctt tcaccagcgc gaggatcacg caatcgagtt
    10201 tgccgctgtc cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga tacatttcca
    10261 gctttggaaa ggtctggtgc agcatcggga taatatgcgg tagcaggtac ggtccaaccg
    10321 tgggaatcaa accaatatgc agcggtccgg acatcgtctc gccctgctgg cttgccatct
    10381 ctttaaggac tttcacctca cgcagcacgg tacgcgcctg atccaccagc agcattcccg
    10441 cctgggtgaa taacacttta cggctggtcc gctccagcaa catcacgccc agctcatctt
    10501 ccagcttacg aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa tctgccgcac
    10561 gccgaaaatg gcggtgttca gccaatgcca ccaggtactc aagatcacga atattcatta
    10621 tccatcctcc atcgccacga tagttcatgg cgataggtag aatagcaatg aacgattatc
    10681 cctatcaagc attctgactg ataattgctc acagaaacgg agcggcacct cttttaaccc
    10741 ttgaagtcac agcccgtttc gagagtttct cagctcgaat aactaaagcc aacgtgaact
    10801 tttgcggatc tccaggatcc gctttttttt gccataaaaa agcccagcga taagccaggc
    10861 tcaaatttat acatataaat gttcttaccc caaccgcgcc tgcgcaaaag caatcgcctg
    10921 cgccacctgt tgcggtgaga caccgccttt tgccgcacgc ttgtcgaggc acgattgcag
    10981 cgacagaatc ggatagacat cttcgccaat caccaggctg aatttctgca actcactgag
    11041 tggcagatct tccagcggtt tgccctgacg aatggcttcc accaccgctt cacccacaat
    11101 atggtgcgcc tcgcggaacg gtacgccttt cgccaccaga taatccgcca gttcagtagc
    11161 gttggagtaa ccctgctgcg ccgcttcctg gcaacgcgga cgtttcacct gaatgccgtc
    11221 cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt cgaacagacc
    11281 ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca gcgtcatcat
    11341 catgccggtt aacgccccct gcacccgacc gcatttaccg cgaatcagct ccagcgcatc
    11401 cgggtttttc ttctgcggca ttaatgatga accggaagtc acacggtcag aaagctccac
    11461 aaaccccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg acagatgcac
    11521 catgccgata gcggcagcag aaagcagttc caacacatgg tcacggtcag aaacgctgtc
    11581 gagactgtta cgggtcgccg aagcaaagcc cagccaacct gctaactgtt cacggtcgat
    11641 ttcataggcc gttcctgcca gtgcgccaca gcctagcggg ctgacatcca gacgcttaag
    11701 cgcatcctgc aaacggcttt catcacgcgc cagcatctca acgtaggcca ggcaccagtg
    11761 cgcgaacgtc accggctggg cgcgttgcag gtgggtgtaa cccggcatca ccgcgtcctg
    11821 attgttctgc gcggtctcga ccagagcgga ttgtaactgc cggttagccg tcagcaactc
    11881 gctaacggta tctttgcacc acagtttcag gtcagttgct acctgatcat tacggctacg
    11941 cccggtatgc aattttttgc ctaactgacc cactttgtcg atcagtttgc cttccaccca
    12001 gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc gaacatcttc
    12061 cagcagcacg ttcagcgcct cttccagttg cgcctgctct tctgcggtta acacgccgac
    12121 cgttaccagg gctttggacc aggccacaga gccaacaata tcctgctccg ccagacggta
    12181 atcaaagcgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct gggtaaaacg
    12241 cccgccccaa agtgccataa ctctgtttcc ttattttgaa attcaatgcc ggaaagctgc
    12301 gcctccggcc aacaaaactt aagctaaaat ccgcgtcccc atcggcatac cgttaaacag
    12361 tgccggaagc tgttccgcat gacgccagga ggcgatatct accggacggc ccaacgtgcg
    12421 cgcagcatcc agcgccgcgt tcactttcac aatcatgccg tcagtaataa tgccctgctc
    12481 aatcagttgt tctgctttcg cggcggtcat ttcggcaata cgttgccctt tgccgtcgag
    12541 aatgccgctg acgtcagaga gcaaaatcag atccgcgccc agcgttgccg ccagcgccgt
    12601 cgccgcctgg tcggcattga cgttcatcag ttgcccttcg tctgttacgc caatggagct
    12661 gaccaccggc agataaccgt tctccagcaa actgttgata agcttaggcg aacctggctg
    12721 cgccagtcca acatggccta actcttcatc aagctgggtc actttgatgc tgtcgccgtc
    12781 accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca gcagggtttt
    12841 atttgccgtt cccgccagtg ctccggtgat aatgtctatc tgatccgcag gcgtcacccg
    12901 caggccattt ttctttttca ccggcagatt cagccctttc atcagctcat ccaccacaca
    12961 accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcgcgataat tcaccagtgc
    13021 gctaaacaga cgttccagcg cctcttcact atccagcagt acgccaccca gtttgataat
    13081 taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag ccgaaacgaa
    13141 tattggcgca ctgcaccgct tgtgccgccg cgcctttcag taagttgtct tcggtcgcca
    13201 caataatcag atgctcgccc tgaacggcaa aaccgatatc gcagaatggc agtccaacga
    13261 catttttcag cgccggaacg cctttgtcat acagccgcac cagtggttta tgggcatacg
    13321 cctgctgtaa cgcttgcgcc acttgcacct ggctcacacc cggtttcagg cggcaggtaa
    13381 tggtttcgag aatgccgcgc gggaaattgc ccagatgcgg ggtgaagata acgtcagcac
    13441 cgaggtgtgt ggcgatctct ggttgatggc gatgggtaaa gacgccatac ggttgcaggc
    13501 taacttcaca aaaactattt gaaatcgctg ccttacgtcc tgcaccgctc acgccgctgg
    13561 tggcgttgat caccggccac tgattgaggt caagaagatc ggcatcaatc aacggtttca
    13621 gcgccagctg tgctgccgtc ggataacagc ctggcacggc aatcaagttc gcttctttta
    13681 atttattacc gcaccactcc gccagaccgt aggctgcctg ttccagcagt tccgggtatt
    13741 gatgggtaaa gccgtaatat ttttcataga aggcgacatc gttaacacga aacgcgccgg
    13801 aaaggtcgaa caccacacag cctgcttcaa gaaattgcgg cgctaaatcg tggctgactt
    13861 catgggcggt ggcgagaaac accacgtcca cccctgggct aaactcgccg atatccgaca
    13921 tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg gagattaact
    13981 ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata tgcggatggc
    14041 gatttacata ggtcactagc tctgcgccag cgtagccact ggcacccaca atcagcgtat
    14101 tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt aaaacattca
    14161 ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc ataaatactg
    14221 catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca aattaccgcc
    14281 atttatcgag atttaccgtg ctctgattgc cacaccttca ataagcgcca cggaagaggc
    14341 actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta aggatttagg
    14401 cttcaatgtg gaagtgcagt ctgtgccagg aactcgcaac aaatttaata tgctggccag
    14461 ttgcggacag ggggctggcg gtttgttgct ggcggggcat accgatacgg tgccatttga
    14521 tgacggacgc tggacgcgcg atccatttac gttgacggag catgacggca agctttacgg
    14581 cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc tacgcgatgt
    14641 cgacgtcacg aagcttgcaa aaccgctcta cattctggcg actgccgatg aagaaaccag
    14701 tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt gcgccatcat
    14761 tggcgagcca acgtcgctgc aaccggtacg cgcacataaa ggccacatct caaatgccat
    14821 tcgaattatg ggtcagtcgg gccactccag cgatccgtcg cgcggggtta acgctatcga
    14881 actgatgcac gacgccatcg gccatatttt gcaattgcgc gataacctga aagaacgcta
    14941 tcactatgaa gcgtttaccg tgccataccc cacactcaac ctcgggcata ttcacggcgg
    15001 cgacgcctct aaccgtattt gcgcttgttg tgagttgcat atggatattc gtccgctgcc
    15061 aggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc cagtgagcga
    15121 acgctggccg ggtcgtctga cggtcgacga gctgcacccg ccgatccctg gctatgaatg
    15181 cccaccgaat cataaactgg ttgaagtggt tgagaaattg ctcggagcaa agaccgaagt
    15241 ggtgaattac tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc tggttctggg
    15301 gcctggctca attaatcagg ctcatcagcc tgatgaatat ctggagacga ggtttatcaa
    15361 gcccacccgc gaactgatca cccaggtgat tcaccatttt tgctggcatt aaaacgtagg
    15421 ccaaacaatg tcggatgcga tgcttgcgca tcttatccga cctacacctt tggtgttact
    15481 cgaggcgatt ttttaacatt cccataagtt acgcttattt aaagcgtcgt gaatttaatg
    15541 gcgtaaattc ctgctattta ttcgtttgct gaagcgattt cgcagcattt gacgtcaccg
    15601 cttttacgtg gctttataaa agacgacgaa aagcaaagcc cgagcatatt cgcgccaatg
    15661 cgacgtgaag gatacagggc tatcaaacga taagatgggg tgtctggggt aatatgaacg
    15721 aacaatattc cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg ggagaaacca
    15781 tcaaggatgc gttgggagaa cacattcttg aacgcgtaga aactatccgt aagttgtcca
    15841 aatcttcacg cgctggcaat gatgctaacc gccaggagtt gctcaccacc ttacaaaatt
    15901 tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag tcagtttctg aacctggcca
    15961 acaccgctga gcaataccac agcatttcgc cgaaaggcga agctgccagc aacccggaag
    16021 tgatcgcccg caccctgcgt aaactgaaaa accagccgga actgagcgaa gacaccatca
    16081 aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac ggctcaccca accgaaatta
    16141 cccgtcgtac actgatccac aaaatggtgg aagtgaacgc ctgtttaaaa cagctcgata
    16201 acaaagatat cgccgactac gaacacaacc agctgatgcg ccgcctgcgc cagttgatcg
    16261 cccagtcatg gcataccgat gaaatccgta aactgcgccc aagcccggta gatgaagcca
    16321 aatggggctt tgccgtagta gaaaacagcc tgtggcaagg cgtaccgaat tacctgcgcg
    16381 aactgaacga acaactggaa gagaacctcg gctacaaact gcccgtcgaa tttgttccgg
    16441 tacgttttac ctcgtggatg ggcggtgacc gcgacggcaa tccgaacgtc actgccgata
    16501 tcacccgcca cgtcctgcta ctcagccgct ggaaagccac cgatttgttc ctgaaagata
    16561 ttcaggttct ggtttctgaa ctgtcgatgg ttgaagccac ccctgaactg ctggcactgg
    16621 ttggcgaaga gggtgccgca gaaccgtatc gctatctgat gaaaaacctg cgttctcgcc
    16681 tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg ccaaaaccag
    16741 aaggtctgct gacacaaaac gaagaactgt gggaaccgct ctacgcttgc taccagtcac
    16801 ttcaggcgtg tggcatgggt attatcgcca atggcgatct gctcgacacc ctgcgccgcg
    16861 tgaaatgttt cggcgtaccg ctggtgcgta ttgatatccg tcaggagagc acccgccata
    16921 ccgaagcgct gggcgagctg acccgctacc tcggtatcgg cgactacgag agctggtcag
    16981 aagccgacaa acaggcgttc ctgatccgcg aactgaactc caaacgtccg cttctaccgc
    17041 gcaactggca accaagcgcc gaaacgcgcg aagtgctcga tacctgccag gtgattgccg
    17101 aagcgccgca aggctccatt gccgcctacg tgatctcgat ggcgaaaacg ccgtccgacg
    17161 tgctggctgt ccacctgctg ctgaaagaag cgggtatcgg gtttgcgatg ccggttgctc
    17221 cgctgtttga aaccctcgat gacctgaaca acgccaacga tgtcatgacc cagctgctga
    17281 atatcgactg gtatcgcggc ctgattcagg gcaaacagat ggtgatgatt ggctattccg
    17341 actcagcaaa agatgcgggc gtgatggcag cttcctgggc gcaatatcag gcacaggatg
    17401 cattaatcaa aacctgcgaa aaagcgggta ttgagctgac gttgttccac ggtcgcggcg
    17461 gttccattgg tcgcggcggc gcacctgccc atgcggcgct gctgtcacaa ccgccaggaa
    17521 gcctgaaagg tggcctgcgc gtgaccgaac agggcgagat gatccgcttt aaatatggtc
    17581 tgccagaaat caccgtcagc agcctgtcgc tgtacaccgg ggcgattctg gaagccaacc
    17641 tgctgccacc gcctgagccg aaagagagct ggcgtcgcat tatggatgaa ctgtcagtca
    17701 tctcctgcga tctctaccgt ggctacgtac gtgaaaacaa agattttgtg ccttacttcc
    17761 gctccgctac gccggaacaa gaattgggta aactgccgtt gggttcacgt ccggcgaaac
    17821 gtcgcccgac cggcggcgtc gagtcgctgc gcgccattcc gtggattttt gcctggacgc
    17881 aaaaccgcct gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa aaagtggtcg
    17941 aagatggcaa acagagcgag ctggaagcca tgtgccgcga ttggccattc ttctcgacgc
    18001 gtctcggcat gctggagatg gtcttcgcca aagcagacct gtggctggca gaatactatg
    18061 accaacgcct ggtcgacaaa gcactgtggc cgttaggtaa agagttacgc aatctgcaag
    18121 aagaagacat caaagtggtg ctggcgattg ccaacgattc tcacctgatg gccgatctgc
    18181 cgtggattgc agagtctatt cagctacgga atatttacac cgacccgctg aacgtattgc
    18241 aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga aggccatgaa ccggatcctc
    18301 gcgtcgagca ggcgttaatg gtcactattg ccgggattgc ggcaggtatg cgtaataccg
    18361 gctaatcttc ctctcctgca aaccctcgtg cttttgcgcg agggttttct gaaatactgc
    18421 tgttctaaca ccctcgtttt caatatattt ctgtctgcat tttattcaaa atctggatat
    18481 cccttcagat atccttaagg aattgtcgtt acattcggcg atattttttt caagacaggt
    18541 tcttactatg cattccacag aagtccaggc taaacccctt tttagctgga aagccctggg
    18601 ttgggcactg ctctactttt ggtttttctc tactctgcta caggccatta tttacatcag
    18661 tggttatagt ggcactaacg gcattcgcga ctcgctgtta ttcagttcgc tgtggttgat
    18721 cccggtattc ctctttccga agcggattaa aattattgcc gcagtgatcg gcgtggtgct
    18781 atgggcggcc tctctggcgg cgctgtgcta ttacgtcatc tacggtcagg agttctcgca
    18841 gagcgttctg tttgtgatgt tcgaaaccaa caccaacgaa gccagcgagt atttaagcca
    18901 gtatttcagc ctgaaaattg tgcttatcgc gctggcctat acggcggtgg cagttctgct
    18961 gtggacacgc ctgcgcccgg tctatattcc aaagccgtgg cgttatgttg tctcttttgc
    19021 cctactttat ggcttgattc tgcatccgat cgccatgaat acgtttatca aaaacaagcc
    19081 gtttgagaaa acgttggata acctggcatc gcgtatggag cctgccgcac cgtggcaatt
    19141 cctgaccggc tattatcagt atcgtcagca actaaactcg ctaacaaagc tactgaatga
    19201 aaataatgca ttgccgccgc tggctaattt caaagatgaa tcgggtaacg aaccgcgcac
    19261 cttagtgctg gtaattggcg aatcgaccca gcgtggacgc atgagcctgt acggttatcc
    19321 gcgtgaaacc acgccggagc tggatgcgct gcataaaacc gatccgaatc tgaccgtgtt
    19381 taataacgtg gttacgtctc gtccgtacac cattgaaatc ctgcaacagg cgctgacctt
    19441 tgccaatgaa aagaacccgg atctgtatct gacgcagccg tcgctgatga acatgatgaa
    19501 acaggcgggt tataaaacct tctggatcac caaccagcag acaatgaccg cccgcaatac
    19561 gatgctgacg gtattttcgc gccagaccga caagcagtac tacatgaacc agcaacgtac
    19621 gcagagtgcg cgtgaatacg acaccaacgt gctgaagccg ttccaggaag tgctgaagga
    19681 ccctgcgccg aagaaactga tcatcgttca tctgctgggt acgcatatca agtacaagta
    19741 ccgttatccg gaagatcagg gcaaatttga cggtaataca gagcatgttc caccgggatt
    19801 aaacgcagaa gagctggagt catataatga ttatgacaat gctaacctgt acaacgatca
    19861 cgtggttgcc agcctgatta aagactttaa agcgaccgat ccgaacggtt tcctggttta
    19921 tttctctgac cacggtgaag aggtttacga cacgccgccg cacaaaactc aggggcgtaa
    19981 tgaagacaat ccgacgcgtc atatgtacac cattccgttc ctgctgtgga cgtcagaaaa
    20041 atggcaagcg actcatccgc gtgatttctc acaggatgtt gatcgtaaat acagcctggc
    20101 ggaactgatc cacacctggt cagatttggc gggcttatct tacgacggtt acgatccaac
    20161 ccgttcagtg gtgaatccgc agttcaaaga aactacccgc tggattggta acccgtataa
    20221 gaaaaacgca ctgatcgatt acgacactct gccgtatggc gatcaggtgg gtaatcagta
    20281 attatcagcc agatgttatt tgccggatgc gacgcttgac gcgtcttatc cggcctacag
    20341 atcgcggcgg taggccggat aaggcgttta cgccgcatcc ggcaatacaa gtcgactgaa
    20401 actgccgcaa tgtttacatc tcactcccat caggccacac ttttcaggtg gccttttttc
    20461 ataatgccag ttactgtttt cccgtctaca tacctaaggg aataacatgt atcacgacgt
    20521 cagctatctg ctctcccgcc tgatcaacgg cccgctgtcc ctgcgccaga tttactttgc
    20581 cagcagcaac ggcccggtgc ccgatcttgc gtatcaggtc gattttccac gtctggaaat
    20641 tgtgctggaa ggtgagttta ttgataccgg cgctggagca gcgttagttc ccggcgatgt
    20701 gctgtacgtc cctgctggtg gctggaattt tccacaatgg caagcccccg ccacgacctt
    20761 tagcgtgcta tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca
    20821 atatcaaaat ctggcgaagc aacacgtcgc ccgacgcggc ccacgcattg gttcttttct
    20881 gctacaaacg ctcaatgaaa tgcaaatgca gtcgcaggag cagcaaacgg ccagacttat
    20941 cgtcgccagc ctgcttagcc actgccgcga tttgctcggc agccaaatac agaccgcctc
    21001 acgcagccag gctctattcg aagctattcg tgattatatc gacgaacgct atgcctccgc
    21061 acttacccgc gaatctgtcg cacaggcgtt ctatatttcg ccaaattacc tctcgcacct
    21121 gtttcaaaaa acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca
    21181 cgctaagaca ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg
    21241 ctttgtcgac agcaactact tctgccgtct gtttcgtaaa aataccgaac gctcgccgtc
    21301 ggaatatcgt cggcagtacc acagccaact gacagaaaaa ccgactactc cagaataaga
    21361 tgggtttgct gcggcgcaga aagcactttg cgcactgcgc tcatcaccct ctgcggctca
    21421 cgcaggaaag cgtagatgct gcattgcacg tagcgacaat gttcaaatcg ctcagcaccg
    21481 gcaagctcga tatccgtaat taacagcgca acatccgccc ggcggatatc ctcgtccgct
    21541 aaacgattct ccgttcccaa cgcgccctga gtttcaatgc tgacgcccca cttctctaac
    21601 tggcacaact tttccagccg ttccgccgcc atataagtat gcgccacgcc gcttacgcag
    21661 gcggttactg ccaccagata tgccatttta acctcccacg gtaacctgaa atccggcccg
    21721 ttctgccatt tcacgcatcg ttgccacatc ggctgacgac ggcgcagcaa cctctttcat
    21781 cgaccatgtt ttccccagca ggcggtattt cggctcgccg tactgatgaa acggtaacag
    21841 atggatctgc ttgatattca gcgggatcag cacgtccagc gcctgctgca tattctcccg
    21901 gctgagcgtg aaaccaggga tcagcggtaa acgcgggatc acgttgacac cctcactcac
    21961 cagcaaacgc agattctcca gcacgcgttg caggttcatc ttcaccacat cccgcgcctg
    22021 agccgcatcc ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagcggtaa
    22081 cagcttgctg gctggcgcgt ctccggcagt ttcgatggcg catgacacac cccatagccg
    22141 cagtcgctgt aaaaaacggg tcgcaaactc cgcctgcatt aacacttcgc cgccagaaag
    22201 cgtgacgccg ccgccggacg tgcgaaagaa aatgtcatct ttcatcactt cccgttccag
    22261 cgcgtcaagg ctgatatcgc ggccaatccg ttcaaatgcc ccggaggggc attcatccgc
    22321 atcacgcaaa catttcgcgc agtgcagaca tttcgcctct ctgcgcaccg tctggatttt
    22381 gccggagatc gactccggat tagcacacca tgggcaaaga tgcggacagc ctttaaaaaa
    22441 gaccaccgta cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg
    22501 cgccacatca tcgcggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga
    22561 tgtcatcctg gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca
    22621 ccaccagccc ggcataatct tgtgggcgct gttgcgcttc ccgtaacgtg tcggcgttca
    22681 ccacgttaaa ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag aagtcggcca
    22741 gtttgcgtaa tcccgcttca ccttcgaggg tcgccggagt gaatttcacg ttcagcaatg
    22801 taccgttaga aagcagtgtg ttatcgagct tactgactga tttcagtacc gccgttggtc
    22861 cttgtgcgtc ctgacccagc atgggtgaca agccaccgtc tgccagttgc tctccggcaa
    22921 agcgaccgtc tggcgtcgcg ccaaccaccg ctcccaacgg gacatgagca gaaacggtat
    22981 acgatcccgg cgtgaagtag ccgccgcgcg ggttctggta tttttccact tctttgcagt
    23041 agtggcgtaa cagttcggcg ctaatgttat ccacctcgtc gatatcgtta ccgtatttct
    23101 cgaagcggtt aattaagcga gcgcggactt tttcgccttc tggcgtggcg aagttggctt
    23161 ttaataccga cagcaattcg tcaaaactta aacgctgttg atcaaaaatc atccctttca
    23221 acgcatggag agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat
    23281 aacgcgcgcc gccatcggta atatcgcgac ctttttccag acaatcgctg ataaaagacg
    23341 agagtagcgg tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa
    23401 ccatcagggt gatgtagtgg ctgatcttgg cgcggatctg ttccagtaaa ccttcataag
    23461 tcagcgcagc gttgccttca ttttcatgca ggcagatttc catcactttc agcaggttaa
    23521 acatcgcgat gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca
    23581 ctacggaata gtcgcgcgca tcttccagca aaacgccacg gttgaggaac gctggcacta
    23641 ccacttcatc gttaaagatt tgcggaatac cggtgccgag gcgaatggtt tctgcggttt
    23701 tcatcaggaa cggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta
    23761 attgcacgct ttgataagcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc
    23821 cgttctcggt taacccgccg agcagtgcgg tatagccggt agggaaaccg gcgaaataac
    23881 gcgcgctact ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga
    23941 gcagttcttt caggaatgcc ggatcttcgc cctgggttaa tgatgtctga tagaacggca
    24001 acatatactg gtcgaagcgc cccaacgata gcgaactggc gttggattcg tattgcagaa
    24061 tgatgttcat gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttcgg
    24121 cgttatggcg ggagatctcc gcaatagtca gcaattcttc gcgacgcggg ccatcagtgc
    24181 agcttgccgc catcgtttcc gccagttcgg cgtaacgcaa aatatgtttc tgcgaggctt
    24241 ccagcagtaa cagcgctgcc tgataaaagt gattctccgg ctgttgctga caatgttgct
    24301 gcatctgtgc caccagctcc cccagcccgt gattcagcag gcgcgggtaa tcaataataa
    24361 tgtgcccctg gcctttatcc gtctggttga tgctgaaaat ctgcgtgctg gtcgcggctt
    24421 ttacttcatc cgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg
    24481 ggaacaactc ttcgcggtag atacgtttat cttcttcgct gatagcaaag cggtcctgcg
    24541 gacgcgtcgg gaattgatcc agttctttca gcagccagta agggtccatt tccggcgaca
    24601 taatcccggc gcgtggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg
    24661 aaatttcaac gtgttcaagg atatacgctg ttgctttcgc ccgacgcatt atcaccggct
    24721 ccccttcggt ttgccgatgg ctggcggtat aaagcaacgc ccgctccagc gagatttcac
    24781 gggtattggc aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct
    24841 gtgggaaagc gaacccgccc ttgcgggcgg gataagaact taagcggttt gtgccaggtg
    24901 agcttcaatt ttgctcataa tggcgtcagc acgctttacc gcgtcgctga tgttgacacg
    24961 cacgatagtc ttgccagcaa aacgctcttc aaatttgatg ccgatatctt tggtcaaaat
    25021 caccatatcg gcgcttgcca cgtcttccgc agtcagttca ttttccagac caatcgaccc
    25081 ctgggtttct acttttactt cccagccttt cgctttcgcg gcgctttcca gcgcctctgc
    25141 cgccatataa gtatgggcaa cacctgaagg acatgcggtt actgcgataa ttttcgtcat
    25201 ggctcgttcc tcaattaatt aatttcaaaa tccagatcca ggtcgtcttc cttctcttca
    25261 gttgaagacc catttttacg cgccagactt ttcagcacgt tcacgcagac tgccgtcaca
    25321 accgccccga cagccactgc cgcgatgtaa cccaacttac cttcaaccac cggcagcaca
    25381 atcagaccac cccagcctgc atagcactgc gcaccgacca gcgccgcagt taccgcacca
    25441 caaactgaac cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga
    25501 atcgcccctt cagtaacgcc aacacagccc atcaccagtg ccgccttgcc ggtttcgcgc
    25561 tcttctgcgg agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggtgga
    25621 acacaaatcc caacagcggc aatagccacc acggtataaa caccctgagc aacgcaaatc
    25681 agcatgaagg cataggccac tttgttaacc gggccgccca tatcgaacgc cagcatcaga
    25741 cccatgatca ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgggtc
    25801 aggctgttgg tcaacgcccc caccggctcg cccagccccc acatcatgac gccagcggta
    25861 atcagtgtgc cgacgattgg aatgatgaag attggcatta ccgagcgcag aactttatgc
    25921 actggaattt tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagcgca
    25981 ccaaagaagc ccgcgccaaa gctgttacca acccaggcac cgatagcgca tggagccagc
    26041 gcagaacgtt ctgcaatgga ataaccgatg taagcggcga ggaaaggcac catcagcgtc
    26101 aaacctgcaa cgccgatatc aaacagtttt ttcagattcg gatcggctac ggcatccggc
    26161 actgcgcctt tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg
    26221 aagggaatca tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc
    26281 accaactcat tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg
    26341 aagaaacaat aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac
    26401 ttttgtaata tcagtacaaa aatgcgatcc gcctcataac ttgcgataat ccggaagtac
    26461 gccgcaaaac ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca
    26521 gcttttggca gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga
    26581 aaaccgtacc gggagaaagg caatggccct gattgtggaa tttatttgtg agctacctaa
    26641 cggcgtccat gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca
    26701 aattgagtgg cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct
    26761 gattggcacc gatacgctgg tgggcgataa ctgccagtta ctgatttccg gggccgacga
    26821 acaggaagcg caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgacgc
    26881 accgctggcg gaagtaaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct
    26941 gaatccgcaa attatccgcg ctcgcaccgt gtgcagcggt agcgcaggcg gcattctgac
    27001 gccgatctct tctttagatc tcaatgcgct gggtaatctt cccgcagcca aaggcgttga
    27061 cgccgaacaa tccgcactgg aaaacggtct gacgctggta ctgaaaaaca tagagtttcg
    27121 tctgctggat agcgacggtg ctaccagcgc gattctggaa gcgcaccgat ccctggctgg
    27181 cgatacttcc ctgcgcgaac atttactggc aggtgtcagc gccggattaa gctgcgccga
    27241 agcgattgtt gccagtgcga atcacttttg cgaagagttc gcccgttcca gcagcagtta
    27301 cctgcaagaa cgtgccctgg acgtacgcga cgtctgcttc cagttactcc agcaaatcta
    27361 cggtgagcaa cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga
    27421 tgaactgacc cccagccagt tcctcgaact ggataaaaat catctcaaag gtctgttact
    27481 gaaaagtggc ggcaccacat cacatacggt gatccttgcc cgttcgttca acattccgac
    27541 gctggtaggt gtggatattg atgcccttac tccgtggcag catcagacga tttatatcga
    27601 cggcaacgca ggggcgattg tggttgagcc aggggaatcc gtggcccgtt attatcagca
    27661 agaagcccgc gtacaggacg ctctgcgtga gcaacagcgt gtctggctga cccagcaagc
    27721 gcgtaccgct gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc
    27781 gcaggccgca ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta
    27841 tatggatcgc accagtgcac cgggtgaaag cgagctgtac aacatctttt gtcaggcgct
    27901 ggagtctgcc aacggacgca gcattattgt gcgcactatg gacattggtg gtgacaaacc
    27961 cgttgattat ctgaacattc ccgcagaagc taacccgttc ctcggttatc gcgcggtgcg
    28021 tatttatgaa gaatacgcgt cgttgtttac cacacaacta cgatcgatcc tgcgcgcctc
    28081 tgctcacggc agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg
    28141 ggtgaaagaa aaactggcgg aagccaaaca gcaactgcgt aacgaacaca ttccgtttga
    28201 tgagaagatc cagctcggca tcatgctgga agtgccgtcg gtgatgttca tcatcgatca
    28261 atgctgcgaa gagattgatt tctttagtat tggtagtaat gacctgacgc aatatctact
    28321 ggcagtggat cgcgataacg ctaaggtgac tcgtcactac aacagcctga atccggcctt
    28381 cttgcgggcg ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat ggattggtct
    28441 gtgcggtgaa ctgggagcga aaggttccgt gctgccgttg ctggtcggtt tagggctgga
    28501 tgaactcagc atgagcgcac catcaattcc ggcggcgaaa gcgcggatgg cgcaacttga
    28561 tagccgtgag tgccgccagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt
    28621 ggaacacctg ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg tcaccgccga
    28681 gtgcatcaca ctggaaagcg actggcgcag caaagaagaa gtgctcaaag gcatgaccga
    28741 taacctgctg ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttgtgggc
    28801 gcgcgaggcc gttttctcta ccggactggg ctttagtttc gccattcccc acagtaaatc
    28861 ggaacacatt gagcaatcca ccatcagcgt agcgcgtctg caagcgccag tgcgctgggg
    28921 cgatgatgaa gcgcaattca tcattatgtt aaccctgaac aaacacgccg cgggcgatca
    28981 acatatgcgc attttctcgc gcctcgctcg tcgcatcatg cacgaagaat tccgtaatgc
    29041 gctggttaac gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact
    29101 gtaaaaggaa acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt
    29161 ctggcacgca tattcccgat tgccggggtg acaactaacc cgagcattat cgctgccagc
    29221 aaggagtcca tctgggaagt gctgccgcgc ctgcaaaaag cgatcggtga tgagggcatt
    29281 ctgtttgctc agaccatgag ccgcgacgcg cagggtatgg tgaaagaagc gaaacgcctg
    29341 cgcgacgcta ttccgggcat tgtggtgaaa attccggtaa cctctgaagg tctggcagca
    29401 attaaaatgc tgaagaaaga aggcattact acgctgggaa ccgcagtgta cagcgccgcg
    29461 caaggattac tggcggcgct ggctggagcc aaatacgttg ctccatacgt taaccgcgta
    29521 gatgcccagg gcggtgacgg cattcgtact gtacaggagt tgcaagcgtt actggaaatg
    29581 catgcgccag aaagcatggt gctggctgcc agctttaaaa caccacgtca ggcgctggat
    29641 tgtttgctgg caggatgtga atccatcaca ctgcccttag atgtagcgca acaaatgctt
    29701 aacacccctg cggtagagtc agctatagag aagttcgagc acgactggaa tgccgcattt
    29761 ggcactactc atctctaaag gagtaattat ggaccgcatt attcaatcac caggtaaata
    29821 catccagggc gcagatgtaa ttaatcgtct gggcgagtac ctgaagccac tggcggaacg
    29881 ctggttagtg gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag
    29941 ctttaaagat gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa
    30001 tgagatcgac cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat
    30061 tggtggcgga aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt
    30121 agcgatcgca ccgactatcg cttctaccga cgcaccgtgc agcgcattgt ctgttatcta
    30181 caccgatgag ggtgagtttg accgctatct gctgttgcca aataacccga atatggtcat
    30241 tgtcgacacc aaaatcgtcg ctggcgcgcc cgcgcgtctg ttagcggcgg gtattggcga
    30301 tgcgctggca acctggtttg aagcgcgtgc ctgttcccgt agcggcgcga ccaccatggc
    30361 gggtggcaag tgcactcagg cagcgctggc actggctgaa ctgtgctaca acaccctgct
    30421 ggaagaaggc gaaaaagcga tgcttgccgc cgaacagcat gtagtgactc cggcgctgga
    30481 gcgcgtgatt gaagcgaaca cctatttgag tggcgttggt tttgaaagcg gcgggctggc
    30541 ggcggcacac gcggtacata acggcctgac cgctatcccg gacgcgcatc actattatca
    30601 tggtgaaaaa gtggcattcg gtacgctgac gcagctggtt ctggaaaacg cgccggtgga
    30661 ggaaatcgaa accgtagctg cgcttagcca tgcggtaggt ttgccaataa ctctcgctca
    30721 actggatatt aaagaagatg tcccggcaaa aatgcgaatt gtggcagaag cggcatgtgc
    30781 agaaggtgaa accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc
    30841 actgctggta gccgaccagt atggtcagcg tttcctgcaa gagtgggaat aatctactcc
    30901 aaactcccgg cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag
    30961 gcctacgcaa tctctgcaat atagtaaatt tacgtacttt tgtagaccgg ataaggcgtt
    31021 cacgccgcat ccggcacgct gtgccagtaa tctgaaactt ccacgttgtt cgggtgtttt
    31081 tttatccgct tctggacatt ttctccacag aacaatcgtt agcccggaag gtcgaatctg
    31141 tgactcttat tttcacggac gaaacagatg aaagcttcac tggcgttatt cagtcttctg
    31201 acggtattta ccagttactc cttaaaatct cccgccgttc caccaaccgt ggtacaaatc
    31261 caggccaaca ccaatcttgc catcgcagac ggcgccagac agcaaatcgg cagcacgcta
    31321 ttttacgatc cggcgtatgt gcagcttacc tatcctggcg gtgatgtccc gcaagaacgc
    31381 ggcgtatgtt ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga tttgcagaaa
    31441 ctggtgcacg aagatatggc gaagaatttt gctgcgtacc cgcaaaaatg gcagttaaag
    31501 cgcccggaca gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg gtttacccgc
    31561 cacgataaaa cacgccccac cagcaagaac cccagtgact atcaagcggg cgatattgtc
    31621 tcctggcgat tagataacgg gctggcacat attggggtgg tgtcagatgg cttcgcccgc
    31681 gacggtacgc cgctggtgat acataacatt ggcgcaggcg cgcaggaaga agatgtactg
    31741 tttagctggc ggatggtggg gcactatcgg tattttgcta aataagagat ctccccacat
    31801 gaagaaaaaa ttcagatcct ttttacactg acggcttctt ttttcttacg gctcaccagc
    31861 gccagtgcca gtacaatcag cacaataccg ctaccttcaa ctgcccccgg attctcaccg
    31921 agcagccacc aggaaaagag tacgccgcaa acgggaacgg ccagtgtgct taagctggca
    31981 atactggcag gcaagttttt caatacaaac aaccataagc tccacgccag cgctgtcgcc
    32041 agaatcgcac tgtaggccag cgcccagaac acggtgggtt gccagtcaat ttcacgttgc
    32101 ggcaccagta aagcgaccac gctcatcacc agcgccgcat acagcatctg ccaggatgtt
    32161 agcgacaata aatccacgcg cggatggcgg gcatacagac gtttagcaac aatcgcgctc
    32221 gccccccaac tcacaccgga gaggattgcc agcatggcac ttttcatcga agagaaatcg
    32281 agttgccacg gctgcaacac caaaaataag ccgaaagcgg caatcagtat cgcgaaatat
    32341 tgcccacgtc gcaggcgttc accgagaaac aacgcggcga aaatcaccac ccagaacggc
    32401 atggtatagc tcaggattgc caccttcccc gctccgccgc tgaccagcgc ccactgtgcc
    32461 aggccaacca tcccgcaggt ttgtaacagg gcaatggcta aggtgtattt aaacggcgtc
    32521 gggcgcattc cgcgaccacg taataaaaga acgatgaata aaacgagagc gccgaaaatg
    32581 cagcgtaagg cggtgaagtc gaaggcaccg atgtagctgg tgacttgctt catgaaaatc
    32641 cagctataac tccagataag tgtgagcaca accaggccac tgattgccag tgggttgctc
    32701 tttcctgcgg cagacatgag agatccagtg tgtagttttc ctgtggacac tatacgccgg
    32761 taaagaaact tcagcgactg catgaaaggc agtcgctgaa gaaggtcaga ttacagcagg
    32821 tcgaaacggt cgaggttcat cactttcacc cacgctgcca cgaagtcttt aacaaacttc
    32881 tcgtgggcat cgctgctggc gtagacttcc gccaccgcac gcaggacaga gttagaacca
    32941 aagaccagat ccgcacggct ggctgtgtat ttcacttcgc cagtttcgcg gtcacggcct
    33001 tcgaacagtt ctttcgattc gtcggtcgct ttccactcgt aacgcatatc cagcaagttc
    33061 acgaagaagt cattgctcaa tacaccaacg cggtcagtga agacgccgtt tttgctgcca
    33121 tcgaagttgg cacccagtac gcgcatcccg cccaccagcg cagtcatttc cggcgcggtc
    33181 agcgtcagtt gctgtgcttt atcaatcaac agtgactcgg tggtggaaac gtccagacga
    33241 gcgcgatagt tacggaaacc gtcagcaatt ggttccagca gttcgaacat ctcaatgtca
    33301 gtctgatcct gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat gctcaaacct
    33361 gcggcgcttg cggctttctc aacgccaacc acacctgcca gcacgatgat atccgccagc
    33421 gaagctttgc cagactcttt ctgaattttc tccagaactg gcagagcacg aacggctgcg
    33481 gcgttcacat cccagtcacg ctgcggcatt aatgccagac gcgcaccgtt ggcaccacca
    33541 cgtttgtcgc caccacggaa ggtagaagca gacgcccagg ccaccgatac cagctcacta
    33601 acagacagac cagaatccgc aatcgcgaat ttcagatcga taatgtcctg ctcggtcggg
    33661 ttgtagatcg gctgcggcag cggatcttgc cagatcagat cttctttcgg tacttccggc
    33721 ccgatgtaac gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca ggcacgagca
    33781 aaggcttcgt tgaacgcctg cggatcgttg aggaaacgac gagagatctt ctcgaactca
    33841 ggatcaaaac gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt cttcgacgga
    33901 tcaaacggat ccgggataat ttccggtgcg tctaccgctt caaactggat tgcgccagcc
    33961 gggctgcggg tctgtaccca ctcatacttg aacaggttct cgaagaaata gttgctccac
    34021 tgggtcggcg tctgggtcca gaccacttcc agaccagagg taatggcatc tgcgccaacg
    34081 ccgctgccgt aagtgctcgc ccagcctaaa ccttgttctt caatcggtgc agcttctgga
    34141 tcaggaccta catttgatgt cggaccggca ccgtgggttt tacccagcgt atgaccaccc
    34201 gcaatcagcg ccacggtttc ttcgtcattc atgcccatgt tgccgaaggt cgcgcggata
    34261 gctgctgctg ccgaaagtgg ttcgccgctg tgatccgggc cttccgggtt aacgtaaatc
    34321 aggcccatct cggttgcacc cagcggtgct ttcgccagcg cttccggatg acggtgagtc
    34381 agccaggctt tttcatcacc ccagttaaca tccagatccg gttcccagac gtcttcacga
    34441 ccggcaccaa aaccgaaggt acggaagccg gagttttcca gcgccacgtt acccgcgagg
    34501 ataaacaggt cggcccagga gattttctga ccatatttct gtttgattgg ccacaacagg
    34561 cgacgcgctt tatcgaggct tacgttatcc ggccaggagt tcagcggtgc aaagcgttgc
    34621 tgaccacgac ccgcgccgcc gcgaccgtcg attgaacggt aagtacccgc gccgtgccag
    34681 gccatacgaa taaacagacc agcgtagctg ccccagtcgg caggccacca cggctgagat
    34741 tctgtcagca gggctttcag atcttttttc aggccgtagt aatctaattt gctgaattct
    34801 ttgcggtagt caaagtcctc acccagcggg ttagaacgat tagaatgttg gtttaacagg
    34861 tcaacacgga gttgatttgg ccaccagtcg cgcgtggttg tgcccccacc tgcgctctgg
    34921 tcgtgaccgc cctgatggaa cgggcatttg ccggtggctg tcgtgttatg gatatcgtct
    34981 gacgtgctca tcaatgtgct cccctctaca gtgttaccgt tacgatacac agcgttagag
    35041 agaagttata attgaattaa tccacggatg cgatagttga gatcttacat attgttggtt
    35101 aaagagatgt agatcaaatt gatcgtaatt aagacttttt gattatgtcc gatttaggac
    35161 aaatgcttta taaaaaaggg ttttagattt gtatccttca agtaatgagg gagaaaacaa
    35221 ggctgataac cttattttcc cgccctcatt tcgaggcagc attttgtgct ctgtttaaaa
    35281 tttgtgaaca ctgtgtgatt ttcacaaaag gcacaatact tataaaccag gtcgaactcc
    35341 cagtgtatgg caaatcgcat aactcatttc agcgcggtta agcgtataga agtggaaatc
    35401 tttcactcct tcacggctta aaatcttcac catatccatg gcgatattcg cgcccaccag
    35461 cttgcgggtt tcagcatcgt catccaggcc gtcgaacatt tgtgccatcc acgccggaat
    35521 acgcacgttg gtcatgtcgg caaatttctt cgcctgttta aagttcgaaa ccggcaaaat
    35581 tcccggaatg atttccacat caatgcctgc cgagacgcag cgatcgcgaa aacgcaggta
    35641 gctttcgaca tcgaagaaga actgagtaat ggcgcggttg gctccggcat ccacttttcg
    35701 tctcaggttg agtaaatcgg cctgggcact tttcgcttct ggatgtactt ccggatacgc
    35761 cgccacggag atatcgaaat ctgccacttc ttttaacagc gtcaccaggt cagaagcata
    35821 catttctggc ttaccacttc ccggcggcag gtcgccacgc agcgccacga tatggcgaat
    35881 accgttattc cagtagtcgc gtgcaatgat ccgcagctcg gcaggcgtcg catcaatgca
    35941 ggtaagatgc ggtgccgctt ccaggccggt gcgatcttta atgcctttaa taatgctgtg
    36001 cgtacggtcg cgctcgccgg agtttgcgcc ataggtcacc gatacaaact tcggtttcag
    36061 gctgctaagg cgatcgatgg agttccacag ggtctgctcc atttcactgg tacgcggcgg
    36121 gaaaaattcg aacgaaacgt taatctgccc ctggacttct gccagactct gattcagggc
    36181 atcccgctgg ctggcgtgaa aaaagctcat accttacctc atcaatcgct tatcgttata
    36241 tgttgtgttg tgcacatcta tccgtttaga cgtccagatg taaagatgaa ggaaaagccg
    36301 aagggcgtca accgaaaaat aaccaatgat ggtgaggaaa aatcagggaa gatgaaaaaa
    36361 actcatctta attagaggcg ataaaagatt tctctatagc gcaatccacc tcgcacgttt
    36421 tgactcatat tctgacaaca cagcgacacc tgggctaatt cgaaaaacaa cgtcctgcca
    36481 ccggatggtg atgttaaggc atctgattcg gcctgcgccc tcgcgtaggc cgaatacagg
    36541 atctgcgcaa tcaacgggta acaaacagtt gagtcaattc agtgcgcact tttatttgat
    36601 atccttcacg cgcataccgt tgagctgttc atccgtaatg gtgttgcccg ctttgaagta
    36661 atcaaccacc gcgtgataaa cgtaataacc gcccgttgtg ttacgcgctt tcccttcggt
    36721 aaaggcggta aaaccatctc caccatcagc aaggaaactc tgagtagcaa tgtgataaac
    36781 cgtcgcatct tcaatgggtt tgccattcaa agtcagtgtg attacccgct gaccaaccgg
    36841 cctgttgctg tcgtacttca tttccaggcc tttcgatacc tgtaaaacgc cattgcttaa
    36901 acttgcgccg tgttccatca gactgcgtaa ttgtttaccc atgagttcca tcgtcaccag
    36961 ttcgttgggg aacggaaacg tactgatgac gccgcccatc gtgatcgccc ctgcggggat
    37021 ctcattgcgg ataccaccag agtttgttaa cgccagttgg gtgtttttac ccgccgctac
    37081 caataaagcg tcagccgcca ggttcccgag ggaagccgat tcaccatagg cacgttttaa
    37141 ttcaaccggc gattgcgcca ccgtttgttg caccacttca tcaagctttt tattccagcc
    37201 gtcgatcacc tgtttcgttt gcggatcggg cttccactcg tcagcgtaaa tggttttaag
    37261 ctcgaagttt ttcaccgtaa aattatgcgg cttctctttg tagtcgagaa ccagtttgcc
    37321 cacatcaatc ccgccgctgt cagtggagag gatgagcgta ttgccgactt taatcggttc
    37381 cggcgtaccc acatgtgcat gtccggtgat caaaatatcc agccctttca cctgacttgc
    37441 cgtctgaata tctttatcca acgcgcgacg cacatctgtg cctcccatac tggactggcg
    37501 ggctggaaca ccttcgtgga tcagggcgac ggtcaaatca accttgcctt tgagttcatc
    37561 gatataacgt tgtagccatt taatttcatc gcgcgcttca atacccacgc gcgtggcagc
    37621 agataccgta tcattaaagg caaatactcc gtgcaaacca ataacgccta ttttcacacc
    37681 gtctttttcg atgatggtat agggcttatc ccagaatgat ttactgctgt tctgataaaa
    37741 aatattgccc tgcacaatag ggaattttgc ctggctcaac tgtaataatg tgttgtccca
    37801 gccgtggtca aattcatggt tgcctattgt gacggcatca aacggcatag tattcataat
    37861 atcgataata gccttacctt tggtcaggct gctgatatat ggcccggtaa aatagtcgcc
    37921 cgcatcaaaa aaccatgtcg ctttattttt tgctttttct tgcttaacca gcgtagtgat
    37981 atttgcccag ccgccaatat cccgtttacc gtcagcgatc cacggcactt tataaggctc
    38041 gacatgtgca tggaggtcgt tggtataaat gatggtgaca tctttagcgc aagcccagaa
    38101 tggcagtgtt aatgcgatac ccgcagcaag tattttaatt ttcataaaca gtccctttca
    38161 gtgagataaa ggattgctac attacgttct gaaagactta tttatgtgag agtaatcaca
    38221 tccgcagggg atagttgccg gacaaaatca acgaccttgt gaaatcgttg gtgatatatg
    38281 tcgactctgt ttacgattaa tgcctgtaag tcgtttggtt gccgtaattt agggcagcca
    38341 acatcgacag attatagctg gcccgactat cgactgggtt atccggcact gcactgtcgg
    38401 gcatgtggca gttacccgcc gttgtttgac gagcaacaat ttcgtgactg gttgacagtt
    38461 cacttatcta cttttgcaac agagaaaggc catttttgcc cggtatgtta tggcacagag
    38521 acgatttgtt atggtcacaa tccacagggt tcgcaacgca ttcaatgccg taactgcaaa
    38581 aaagtatgga cacccaaaca atatcagaaa gaaataacga ctcccgaaat aatcgaaacg
    38641 gttgctttgc tcgtgccatt tcagggagtc agcagcgggc agaaactgta tgttttaatc
    38701 agttttgatg ctcttcgcgg taatattctt catctttcta ctaattacac gcagcatcag
    38761 gctggagaaa gtctgcatta tcgatacaga ggaaatgcag aaccagagtt acacgacaat
    38821 aatattgtac aacgcgttga tatgcgcgaa gcacaatttc tacgccgcag tcagtttgat
    38881 gaaattcagt atggcagcgc agcgctcaag cgcaatgcca aaggcgctat tctgcgtccg
    38941 gttatcactg cacacgggca tttcagagta ctgaatattt tattccctac agtaaaaaca
    39001 catgtaattt cacacgagtg ttttttaaga ggtgccatta ttacggcctg ggctgatctg
    39061 tttcgccaac agcaaggtga aatttggttt attgaagagg aaatagctga cgataccgat
    39121 aatatgccct ggcgttttca gggaaaaact tatcacggct ggtggaaaaa tcagtggcaa
    39181 ctctgggttc agggaaaaaa tcgtaaaatg gtatgtgcat taacgggcgg aaagagtagc
    39241 aaagcagaaa tgctctcttt agcaaccagc agacatttta tcgactggct acataaacaa
    39301 gcagtattta ctcacagcgc cccgctttcc gcagggcgcg ttacgcaaat actgctttct
    39361 ctggctcagg attacaacaa ctgcgccagt cggttaatat ccgactgaat cgccccggcg
    39421 gtgacgtcgc gccctgcacc tggcccgcgg atcaccagcg ggttgtcgcg ataccagcgg
    39481 ctttcgatgg caaagacgtt atcgcatggc agtagtgatg ccagcggatg gtcttcacgc
    39541 accgcttcca cgcccacacg cgctttgcca ttggcatcga aacgcgccac gtagcgcagc
    39601 accagcccca tttcgcgggc cgcttccagt cgttgcacca tctgctcgtt aagttcatcg
    39661 ccattttcaa agaaatggtc gatgctgccg ccttcgcaat gggcaggcac cagtgattca
    39721 acacgtacct ggtccggttc aatgttgtaa cccgcttcac gcgccagaat caccagcttg
    39781 cgcattacgt ctttgccgga aaggtcatca cgcgggtcag gttcggttaa gccttgctgc
    39841 cacgcctgat ccaccagttc ggtaaacggc acgctaccgt cgaattgcag gaacagccag
    39901 gagagcgtgc cggagaagat cccactgatc gacaaaatgg tatcgccgct gtcgatcaga
    39961 tcgcgcacgg tatggttgat cggcaaaccc gcaccgacgg tggcgttgta cagccagtga
    40021 cgcccggttt tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gcttgctcct
    40081 gccagtttgt tggcactgat gacgtggaaa ccgtggctgg cgaaatcaag atactggtca
    40141 gcgagctgct ggctggcggt aacgtccagc accactaaat catcatacgg atgggcgcgc
    40201 atccacagga acagcgactc ttcatcctgc tcgaccgctt catcgttgaa gaaggctaac
    40261 gcgcggctgg cgtccagacc gtcatagctc aacaggctac ggcggctgtc caccacgcct
    40321 gccagcacaa attcaaagcc ggtacgtgcc gaaagcgtac tctgctcacg ggcgaacagt
    40381 tccagccagc gggaaccgat attgcccttg ccgaacaata ccaggccgat gcgtttttcc
    40441 gcgcggaaga cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt
    40501 actgccacca ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg ctggcctttc
    40561 agttgctgcc agaagcggtg gcaatgcagc gggttacggg tgacgcccgc cccaaccatc
    40621 gccaccagcg ccagcccctg acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg
    40681 attttcagcg cactgtcggc cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg
    40741 ttatgcacgc caaccgccag tggacgtacc tgagcacgtt tcaggatctg gtcgatctct
    40801 ttatgcgcca gtttgaaatc ctggctggcg ggcacctgaa actcaatcaa gcagacatca
    40861 tcatggctgg tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg
    40921 gaaccttgat ccggcgtgta gctacagcgc agttgcaggt cgatttcgct gccagaaacg
    40981 ggctgtaaag tacgggcatg aagaacggga gccgccagac gcgccagttc gctggcttca
    41041 tccagacgca gcaacggcag caggcaggca tctttcactt tacgcgggtc ggcgctatat
    41101 accccggcga cgtcgctcca gatggttacg cgagaaacac ccgccagcgc accgatttgt
    41161 gtcgcggaat agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttacgg
    41221 ctgataaacc cggtcaccac cagacgtttg cccggatgtt gtaccagtag ctgttgcagc
    41281 aacgggtaag aaagcccttc atcaacctgc ggttgcgcag cgcgttcagc gcgtaaaaac
    41341 tcgcgggcat caagccaggc cgatggcagc ccttgttgat taagcaccgc agacatcagg
    41401 cgcgccgacc acacttcacc gtggcccacc acttccgcat acaccgcatc gttaataccg
    41461 ctgtcgagca acgccgccag gcgctcaaga tcgctgacaa aagcgctaat gaggttatcg
    41521 gcttcttcgg cgggtagcag gccgctaatc agatcgcact gataacgacg taatgtttgt
    41581 tgaacctgat gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg
    41641 ttagtggtgc taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc
    41701 ataatgcccg cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta
    41761 tgcagttgac gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg
    41821 cagcccggaa gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg
    41881 agatacgcag cagcgtctcg gagatcccgg cggcagcacg cgcttctggt gccatgcctg
    41941 catgtgtcat ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca
    42001 gcgtaaacaa cgacagcccg cccaggaaac gacgcagcgt ctgctcatcg ccatccagtt
    42061 caaaactcaa cattgcgcca aagccttttt gctggcgcgc ggcaatttca tgcccctgat
    42121 tttccggcag cgacgggtga tacagtttct tcaccaacgg ctgggtttgc agatatttca
    42181 caatcgcctg cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc
    42241 gcagcagcag atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg
    42301 caagttcagt gacaacgtcc gggtctttag caatcactac gcctgccacc acgtcagagt
    42361 gaccgttcag atatttcgtg catgaatgca acaccagatc ggcacctaat gccagtggat
    42421 tttgtaatgc cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg
    42481 ccagatggca aattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta
    42541 ccagtaccag tttgggtttt tctgccagcg ctgcccgtaa tgcctgttca tcgctttgat
    42601 caacaaacaa cacgcgatag caaccgcgtt tcgccaggct gtcgaacagg cgatagctac
    42661 cgccgtagca gtcgtgcggc gcaaccagca gatcgccagg tttcaaaaag acggtcgtta
    42721 ccaggtgaat cgcggacatg ccggtattag taagtacggc accggcaccg ccttccagtt
    42781 ctgccagcgc acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg
    42841 cacgtggttc attaaatccg gtaaagttat aggtactgga aagatggatg ggagggacaa
    42901 cgcaaccata ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac
    42961 gcgtcatgtg atgaagttcc ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca
    43021 ttgtgttaat ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca
    43081 aatcccaaat agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg
    43141 tcacggtaac gcctgtacgg taaactatgc gggtttacgg tcagtaccca cattaactgt
    43201 gtggtctggt ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg
    43261 cgaatatatc agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac
    43321 ggtttccatt cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt
    43381 gaacaacctg cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt
    43441 taccgggcaa cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg acgaaattcc
    43501 ggaggcggca aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta
    43561 aagcggaaaa gagcggcgcg gagttaacca gccgcttttt tcatacatgg acgtttaacg
    43621 atgaaaatca ggctactgat ccccggcctg ctggtttctg tgcctgcatt tgcctggcaa
    43681 ccacaaaccg gcgacatcat ctttcagatc tctcgctcat cgcaaagtaa agcgatccaa
    43741 ctggcgaccc attccgatta cagccacacc ggtatgctgg tgatacgcaa caaaaagccc
    43801 tacgtctttg aagccgtcgg cccggtaaaa tataccccgc tgaaacagtg gatcgcccac
    43861 ggtgaagata gcaagtacat cgtgcgtcgc gttaaaggcg agctaaatgc cgaacaacag
    43921 caaaaactgg cgcaaacggc aaaacgttat ctcggtaaac cgtatgactc cagcttctcg
    43981 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtctacca gaatgcgctg
    44041 ggaatgcgtg tgggcgagca gcagaagctg aaagagttcg atcttagtaa cccgctagtt
    44101 caggcaaagc tcaaagaacg ctacggcaaa aatattccgc ttgaggaaac ggtagtttca
    44161 ccacaagccg ttttcgacgc gccacaactc accacggtcg ccaaagaatg gccgctgttt
    44221 tcctggtaat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    44281 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    44341 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    44401 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    44461 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    44521 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    44581 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    44641 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    44701 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    44761 gctgccagaa ggcatgacgg ttaaagccgg gagtcgcgtg cgcgtgccgt ttggcaaaca
    44821 acaggagcgc atcggggttg tggtgtcagt gagcgatgtc agtgagctgc cgcttaacga
    44881 gctgaaagcg gtagttgaag tgctggacgt tgagccggta tttactcact ccgtctggcg
    44941 attactgctc tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    45001 cttgccgatt ttactgcgcc aggggcggcc tgcggcaaac gcgcctatgt ggtactggtt
    45061 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgttcgc caaagcaaca
    45121 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gatcaggttg ccacgctcga
    45181 atttaatgat gccgcgttgc aggcactgcg caaaaaaggt ctgtgtgatt tagcaagtga
    45241 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcggtt
    45301 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    45361 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    45421 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    45481 gcaaactatc gcccgttttc gtgaacgttt taatgccccg gtggaagttc tgcattccgg
    45541 cctgaacgac agcgagcgtc tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat
    45601 tgtcatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgttat
    45661 tgatgaagag cacgacagtt cctacaaaca gcaggaaggc tggcgctatc atgcccgcga
    45721 cctggcggtg tatcgcgcgc atagcgagca aatcccaatt attcttggct ccgcaacgcc
    45781 cgcactggaa acgttatgca acgtgcagca gaaaaaatac cgcctgctgc gcctgacccg
    45841 tcgggcgggc aatgcgcgcc cggcaattca acatgtgctg gatttaaaag gtcagaaggt
    45901 gcaggcaggt ctggctccgg cgttgatcac ccgtatgcgc cagcatttac aggccgacaa
    45961 ccaggttatc ctctttctta accgccgggg ttttgcgcct gcgctactgt gccacgactg
    46021 tggctggatt gccgaatgcc cgcgttgcga tcactactac acgctgcatc aggcacaaca
    46081 gcatttgcgc tgtcaccact gtgacagcca acgcccggtg ccgcgccagt gcccttcctg
    46141 cggttccaca cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgctcgc
    46201 gccgctgttc cccggcgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    46261 ggcgctggaa cagcaactgg cggaagtgca tcgcggcggc gcgaggatct tgattggcac
    46321 gcaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtagcgt tactggatgt
    46381 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    46441 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca
    46501 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg actttgccga
    46561 acaggcgctg gctgagcgac ggatgatgca gctgccgcca tggaccagcc atgtgattgt
    46621 gcgtgcggaa gatcataaca atcagcacgc gccgctgttc ctgcaacagt tgcgtaatct
    46681 gatcctcgcc agcccgcttg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    46741 ggcccctaag cgtggtggcc gctggcgctg gcaaattttg ttgcagcacc cttcccgcgt
    46801 gcgattacag cacatcatca atggtacgct ggcgcttatt aatacaatac cggattcccg
    46861 taaagtgaaa tgggtgctgg atgtcgatcc catcgagggc taaaccgctc acgatgcgag
    46921 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    46981 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    47041 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    47101 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    47161 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    47221 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    47281 ccggatatct gcgacccttt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    47341 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    47401 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgttgctggg ttcaaggctg
    47461 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    47521 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    47581 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    47641 gagatgccgc tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc
    47701 attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaga
    47761 gcaatgcaac agctgcttga tcttccacaa ccgcctactg ctgtcttctg ccatagcgat
    47821 gtgatggcgc tcggcgcact ttctcaggca aaacgtcagg ggctgaaagt cccggaagac
    47881 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    47941 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    48001 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    48061 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    48121 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    48181 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    48241 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    48301 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    48361 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    48421 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    48481 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gccagcttct
    48541 tgaacaaatg caggccgata tgcgccagca gccaacgcag ctggttgaag ttccgtggaa
    48601 tgagcagacg ccggaacagc gtcagcaaac gctacagcgc cagcgtcagg cgcagcaatt
    48661 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    48721 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc
    48781 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    48841 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    48901 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    48961 gacggtacgt gctcagctgg cgttcgaagg ctttgactca aaaattacca ccaacaatgg
    49021 ctggaatcgt gtggtgattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    49081 tcgcttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    49141 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    49201 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    49261 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    49321 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    49381 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    49441 ctggtcaaag ccgccgttga gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa
    49501 cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
    49561 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    49621 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    49681 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    49741 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    49801 gaactggata agcacatcat cggccaggac aacgccaagc gttccgtggc gattgctctg
    49861 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    49921 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    49981 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga agtgggctat
    50041 gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    50101 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    50161 ctcgacgtac tgatcccacc ggctaaaaat aactggggac agaccgaaca gcagcaggag
    50221 ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    50281 gaaattgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    50341 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg tcagaagcaa
    50401 aaggcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    50461 aaactggtga acccggaaga gctgaagcaa gatgctatcg acgctgttga gcagcacggg
    50521 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    50581 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg cactgtttcc
    50641 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    50701 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgag
    50761 ctgcaggcac tgaccaccag cgactttgag cgtattttga ccgagccgaa tgcctctatc
    50821 accgtgcagt acaaagcact gatggcaact gagggcgtaa atatcgagtt taccgactcc
    50881 ggtattaagc gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    50941 gctcgtcgtt tacataccgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    51001 gatttaagcg gtcaaactat cactattgac gcagattatg tgagcaaaca tctggatgcg
    51061 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    51121 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    51181 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    51241 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccactggtc
    51301 gccctgctgg cattgattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    51361 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    51421 caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac cgtcgtgctc
    51481 atctgtctct ccgggctggc actggttgca gtggcgtgcc atacgctggc cgattttgtc
    51541 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    51601 cgtccttatg gttatatcgg tttgggtgat atttccgtac tggttttctt tggctggttg
    51661 agcgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    51721 gcaaccgcat gcggcctgct ggcaacggca gtactgaaca ttaataacct gcgtgatatc
    51781 aatagcgacc gcgaaaacgg caaaaacacg ctggtggtgc gcttaggtgc agtaaacgcg
    51841 cgtcgttatc atgcctgcct gctgatgggc tcactggtgt gtctagcgct gtttaatctc
    51901 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccgtt gctggtgaag
    51961 caagctcgtt atgtgatgcg cgaaatggac ccggtggcga tgcgaccaat gctggaacgc
    52021 actgtcaaag gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    52081 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    52141 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    52201 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    52261 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    52321 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    52381 gaggttctgt tcgccgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    52441 aatgggaagg cctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    52501 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    52561 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    52621 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taaaaaaggc
    52681 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    52741 aagatgaagt gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    52801 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    52861 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    52921 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac
    52981 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaatcct
    53041 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    53101 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    53161 tcatccttct cctggtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    53221 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    53281 cattttatga cggagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    53341 ataagtttac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    53401 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    53461 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    53521 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    53581 gcaatggcca tctacctgac cgcaggggtt tccggtgcgc atcttaatcc cgctgttacc
    53641 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    53701 caagttgccg gcgctttctg tgctgcggct ttagtttacg ggctttacta caatttattt
    53761 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    53821 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    53881 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    53941 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    54001 tctatgggcc cattgacggg ctttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    54061 tttgcctggc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    54121 ttcctggtgc cgctttttgg ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    54181 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggagaccaca
    54241 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    54301 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    54361 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    54421 aggctgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    54481 agtgctggcg aaagctgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    54541 gcgtgaaacc actattgtct gggaaaaaga aaccggcaaa cctatctata acgccattgt
    54601 ctggcagtgc cgtcgtaccg ccgagatctg cgaacatctc aaacgcgatg gcatggaaga
    54661 gtacattcgt aacaacaccg gtctggtgat tgacccgtac ttctctggca ccaaagtgaa
    54721 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgcg cgtcgtggtg aattgctgtt
    54781 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    54841 ttacaccaac gcctctcgta ccatgttgtt taacatccat actctggact gggacgacaa
    54901 aatgctggag gtgctggata ttccacgcga gatgctgcca gaagtgcgtc gttcttccga
    54961 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    55021 cgccggtgac cagcaggcgg cgctgtttgg tcagttgtgc gtgaaagaag gaatggcgaa
    55081 gaacacctac ggtaccggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    55141 agaaaacggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtaa actatgcgct
    55201 ggaaggtgcg gtgtttatgg cgggcgcgtc cattcagtgg ctgcgcgatg agatgaagtt
    55261 gattaacgac gcctacgatt ccgaatattt tgccaccaaa gtgcaaaaca ccaatggtgt
    55321 gtatgtggtt ccggccttta ccgggctggg tgcgccatac tgggatccgt atgcacgcgg
    55381 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    55441 gtctattgct tatcagacgc gtgacgtgct ggaagcaatg caggccgact ccggtattcg
    55501 tctgcacgcg ctgcgcgtgg acggcggtgc ggtggcgaac aatttcctga tgcagttcca
    55561 gtccgatatt ctcggtacgc gcgttgagcg cccggaagtg cgcgaagtca ccgctttggg
    55621 tgcggcctat cttgctggtc tggcggttgg cttctggcag aatcttgacg agctgcaaga
    55681 gaaagcggtg attgaacgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    55741 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggca tgggaagaac acgacgagta
    55801 ataatgattg cctgatgcga cgcttgcgcg tcttatcagg cctacgagtc ggatacacat
    55861 ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatcccg
    55921 cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    55981 gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg cgctggctgg ctacaaatgg
    56041 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    56101 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    56161 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    56221 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    56281 ctggcagtag gcgataaagg ctgtttcctc aacgcgccag atatgtatat ggagaagctg
    56341 atcgtcggac cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    56401 cgcaatgttg cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggca
    56461 aaaccgcgcc acgatgccgt tatcgctgaa atgcaacaac tcggcgtacg cgtatttgct
    56521 attccggacg gcgacgttgc agcctcaatt ctcacctgta tgccggacag tgaagtcgat
    56581 gtgctgtacg gcattggcgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca
    56641 ttagatggcg acatgaatgg tcgtctgctg gcacgtcatg acgtcaaagg cgacaacgaa
    56701 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    56761 ggtaaagtat tgcgtttggg cgatatggcg cgcagcgaca acgtcatctt ctctgccacc
    56821 ggtattacca aaggcgatct gctggaaggc attagccgca aaggcaatat cgccaccacc
    56881 gaaacgctgc tgatccgtgg taaatcacgc accattcgtc gcattcagtc catccactat
    56941 ctggatcgta aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag
    57001 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    57061 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    57121 tggaccgacg ccctgtttag tctcaccgtt cacgctcccg tgcttccgtt taccgccggg
    57181 caattcacca aacttggcct tgaaattgaa ggcgaacgcg ttcagcgtgc ctactcttac
    57241 gttaactcgc ccgacaatcc cgatctggag ttttacctgg tcaccgtccc cgatggcaaa
    57301 ttaagcccgc gactggcggc gctaaaaccg ggtgatgaag tgcaagtggt tagtgaggcg
    57361 gcaggcttct ttgtgctgga tgaagtccct gactgcgaaa cactgtggat gctggcgact
    57421 ggtacggcga ttggccctta tttatcgatt ctgcaattgg gcaaagattt agaacgcttc
    57481 aaaaacatgg tgctggttca cgccgcacga tatgccgccg acttaagcta tctacctctg
    57541 atgcaggaac tggaaaaacg ctacgagggg aaactgcgca ttcagacggt ggtcagtcgg
    57601 gaaacggctg cgggttcgct cactgggcgg ataccggcgt taattgaaag tggtgaactg
    57661 gaaagcgcgg ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    57721 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    57781 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttgtcga
    57841 taaacggcac gatcaagaga tccgcactca acttattgat catcccgcga tatgccggca
    57901 tcaaacggtt gataaatgag tggtgatgac cacagacaag gaggtcgcac tgctcttttt
    57961 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    58021 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    58081 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    58141 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    58201 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    58261 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    58321 catctcggcc tggcatgaga aggtgaaggc cgcatcaagc tcaacttgcc cttccagatc
    58381 tttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc
    58441 tttcagtttg gtcataaagg actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    58501 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    58561 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    58621 gacttaagaa taatgcgccg ttaaaggaag aacaaacaag taatgatctt atggattacc
    58681 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggc attgtcactt
    58741 gcatcgtgga aataacaatc ccggaaaaaa atagcgataa gagatatatt aaaggttaat
    58801 tacttgattt attgtcggct ttatacttca catcctgggt atctttacca tatttatttt
    58861 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcagtgttg
    58921 ggataaaacg ccccaccgcc cattgccata cccccggcaa aattgcccag ttacccgcca
    58981 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    59041 agcgcttaac ggttactgcc gctgttggcc aaagcaagca cacaaggcaa aacgccgcgg
    59101 tctgaatatc gagtaaattt ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    59161 acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc
    59221 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tccaggattc tacccttttg
    59281 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    59341 agccagggtg tacgctgttt attctcttat gttctgcatt aaccgttaca acaacggcgc
    59401 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    59461 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    59521 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgcagcaa
    59581 gtaaaattaa cgagaacttg tatgcttcta ctgcgctgga gcgcggtacc ttaaaaatca
    59641 aaagcattca aataacctgg ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga
    59701 aagctcaggc atacatggca gcggtgatcc gcacactcac cccattaatg accaaaactc
    59761 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    59821 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    59881 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    59941 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    60001 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    60061 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    60121 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    60181 catcgatatg gcgaagcgtg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    60241 ggacctgaac ctgtccggcg cattcaccgg tgaaacctct gctgctatgc tgaaagacat
    60301 cggcgcacag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagaatctga
    60361 cgaactgatc gcgaaaaaat tcgcggtgct gaaagagcag ggtctaactc cggttctgtg
    60421 catcggtgaa accgaagcgg aaaacgaagc gggcaaaact gaagaagttt gtgcacgtca
    60481 gatcgacgcg gtactgaaaa cccagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    60541 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    60601 tcacaaattc atccgtgacc acattgctaa agttgacgct aacatcgctg aacaagtgat
    60661 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    60721 catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg ccgtgatcgt
    60781 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    60841 cacttacttt cctaaactcc tcgccttaac gcaaaatctc acactgatga tcctggattt
    60901 cttcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc aatacaaatg
    60961 agttatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    61021 gcacttcttc cgccagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    61081 gacgcgccag gtactcatgc ccacgcaaac cacctggcag tggcaaccag cggctactga
    61141 tgttcgccag attgttatcc agctgttcgc gcacatcagg acgaatacaa gaaatatgaa
    61201 tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccagagat accgcgcgat
    61261 cgggaacagg ctggccgtat ttttggctca taaaatcacg cgcctgccag gctaaccaga
    61321 aaaaattagg cgttgacgga tcggtcaaca atgggctttc agtaccgtta atacggtacg
    61381 ttggcattaa cagatattgc agtgggccat gacgatcttt taaaaccacg tatccggcgt
    61441 tgggtttgac ttccgcacat ggcgacgggt tttggttttc ctgctggttg ggcaaacatt
    61501 gctcaagaac tatcttacgt aacgtgtctg actcttcacc ggttaatttc cagtaaccaa
    61561 taccggcagt cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    61621 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    61681 aacccagtgg cgagaaacca ccgggcttga taattatcct ggggaaatca gcgtttgctg
    61741 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    61801 aactcttcat caatggtgaa taacttcaat tttggaaacg cattttcgta ctttttcgct
    61861 acctcagcgt cgcgcggacg gtagtagttt ttcgctgcaa tttcctgacc ttctggcgag
    61921 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    61981 tcgaccaccg acacggttgg ctctgcgagg atagactcac tcggcgtgac gatttcgaat
    62041 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc aatcagtaca
    62101 tcgccaattc cgcgctcgac aaaagtgtta gtggatccac gcgcgccaga atccagaact
    62161 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    62221 tgatgaagcg cgtagcccca ggctgccagg taattccagc gcgcgccacc agagcttttc
    62281 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    62341 ttcggattac ccttacgtac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    62401 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaattgc gtccacgtca
    62461 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    62521 ttgcctgaac caccgtgtga ctgacgaatc accacgttat cgccagtttg ctgtttccag
    62581 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    62641 ttaagaagct gaatatcctt tgccataacg ctggttgcca ccagcaaaaa tgttaaccct
    62701 acgccccact ttttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    62761 agaaggttga ccaaacatta aagaataaaa aaagattggc tataacttgc gagtatatgt
    62821 tgggggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    62881 atgccggatg cggcacaaac gccttatccg gcctacaaaa gtttgcaaat tcaatagatt
    62941 acagaattca tgtaggcctg ataagcgaag cgcatcaggc ctttttgctt ctgtcatcgg
    63001 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    63061 cagccagtcg cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    63121 aaccagctgt tcgttctgga tacctacgca acgaccaccg taacctgcca gcagcagatc
    63181 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    63241 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    63301 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    63361 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    63421 cggaaccaca acgaattcac agccaccggc aatggccgca gccaacgtca gatcgccaca
    63481 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    63541 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    63601 gcctttgatg tcgttgtcga tagtgcccgg caggccgatg cacgggaagc ccatttcggt
    63661 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatccc
    63721 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    63781 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagaaacgc tgtaacggtc
    63841 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatgc ccattacttc
    63901 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    63961 atcaccgccg cttgtcaaca caccgatttt cttaatcatg actacctctg aactttggaa
    64021 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    64081 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    64141 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    64201 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    64261 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    64321 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    64381 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    64441 ttcctcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    64501 cgcgctatat aaaatataga tgccgatccc caatgcaaac agagcatcgg cgcgatgcca
    64561 gccgtaccag gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    64621 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaacgctg
    64681 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    64741 tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa acaggaatag
    64801 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    64861 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    64921 caacgacgca ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    64981 ggtataccac catgcaaaaa ttttaatcag caatagcagc gaagccatcg ccgtcgcagc
    65041 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    65101 ctgacggtag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    65161 gataaaaaaa acccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    65221 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    65281 tttgccattg cgtcacgtcg cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    65341 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    65401 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    65461 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    65521 gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    65581 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    65641 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    65701 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    65761 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    65821 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    65881 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    65941 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    66001 ctggacgaca gcattgattt acttttgctt gatgtaatga tgccgaagaa aaatggtatc
    66061 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    66121 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    66181 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    66241 agcgaacaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttaagt
    66301 ctcaatcctg gtcgccagga agccaccttc gacgggcaaa cgctggagtt aaccggtacg
    66361 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgagcat
    66421 ttaagccagg aagtgctggg caagcgcctg acgcctttcg accgcgctat cgatatgcac
    66481 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    66541 ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    66601 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    66661 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag ggtctgatga
    66721 ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    66781 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttactggtga
    66841 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    66901 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    66961 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    67021 ccagcagttc tcaatccgat ttcataaact tactgtttga ccgcccgcta ttactgctga
    67081 ttgtcaccat gctggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    67141 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    67201 cggaactgga agcggggcca caggaattcc ttgccgccgg tgccagtttt aaccagatgg
    67261 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct actttctgat atctctcacg
    67321 aactgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    67381 gtgaaagcaa ggaactggag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    67441 acgatctgtt ggtgatgtca cgtaatcagc agaaaaatgc gctggttagc gagaccatca
    67501 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg
    67561 gcaagtcgtt gacggttaac ttcccacctg ggccgtggcc gctgtacggc aacccgaacg
    67621 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    67681 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    67741 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    67801 cacgcgatcg tgaatctggc ggtacaggtt tggggctggc gattgttgaa accgccattc
    67861 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    67921 tttggttgcc gctgtataag cggtcataac gtcaaccttc aggtaaaaaa atgcctgatg
    67981 cactacgctt atcaggccta caaaacctgt tgaatttatg ggttttgtag gctggataag
    68041 gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta
    68101 tcaggcctac actccggggt tattttcccc acaaacggcg ggaaaaatct tcgatcttgc
    68161 cgctcagtcg gcgcttttgc atcgttcgcg tccagctttt cgataaccct gcaacggaga
    68221 gtaaacggtg atactgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct
    68281 gcacggtcac atcgctgaca cgggaaacca actccagtgg cgcatcagca gataccaacc
    68341 ctggtgcaat tacgctatac aaccacccca ctttacccgt gttttgcatc aactgcgcta
    68401 tatcgctgat atcaaaatgg taattgagtt tatagcaagg tgagcgtggc tggctgacct
    68461 gaattaatgc ctctccccag cggaaaatat caccaatata aacattactt tccgtcagac
    68521 cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa cagctccgcc tgttccggaa
    68581 attcccgcgc ccagtagaga taatgctcac gaggataatg acacagcgct ctgtctggcc
    68641 cgccgtggat ctttttctcc gcctgttcgt caccttccag ccccaggtcc gtcagcatca
    68701 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
    68761 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
    68821 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtaagt atccaggcag
    68881 aaacgcaatt gaaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagctg
    68941 gtttatttca ccgtacctaa aatttcgatt ttcgccgctc tggctttgac tttacgtgac
    69001 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
    69061 gaagttgctg ccggagccat aggtttaaac gcaggcacca tttctgcaat cagcacaggt
    69121 gtcgctaccg cggccccagc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
    69181 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
    69241 aacaggatcc ccagcaaacc tgtctgggca attacagtca aatcaatggt attgcccagt
    69301 gcgaaggcaa agaatggaat cagcgtctgc accgctttgc tgaaaaattc tcgtaactca
    69361 gggtcaaggt tccccagggc aaagcccacc aggaacggca atactgcgcc gacgaaaaca
    69421 tgcggttcaa acgaggcaat cccggcagtg cccagaataa tcatcgtcat gagcggcccg
    69481 gactccaacg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    69541 atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc caacgttgaa
    69601 agtccggcaa aaaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaattgcc
    69661 gcaaccaccc acgcgacggc aattttagtt accaccagcg taccggattt acgcagtacc
    69721 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    69781 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    69841 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    69901 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    69961 tgcgtcattc ctcaacggag catcagtgtt gatggaaacg aagataaaca ttcatttgat
    70021 caataaaagt gatcaaaacc acatcaattg aaacgatgtt ttatttttat cggatcgttg
    70081 tttttatttt gaaagtgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    70141 gctgcaatgc ggcgtaaacg cctcattgca gcaggcgtca aatgattatt ttttcgccgc
    70201 gaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttaa tgtagtccgg
    70261 acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccacaatcgg
    70321 gaagccggaa gcgccagaaa tggcttcgcc catcagcgga gagtcctggt tagcagtaga
    70381 aaccaccgcc agtttatcgc ctttttgcac cagccatgcc cagccggaac caaagcggga
    70441 agctgccgct ttttcaaatt ctgctttgaa attatcaacg gaaccgaaat cacgttcgat
    70501 agccgctttc aggtcgccct gcagggtggt gccttttttc agacctttcc agaacagact
    70561 gtggttagcg tgaccgccag cgttattgcg cagtacggtt ttcttgtctg ctggcagctg
    70621 gtccagtttg gtgatcagct cttcaaccgg caggttagcg aattctggca ggctttccag
    70681 cgccgcattg gcgttgttaa cgtaggcctg atggtgtttg gtgtggtgga tttccatggt
    70741 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    70801 actcatattc atctccagtg tggtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    70861 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcataagg
    70921 gtatggtttt gcaggaaaat gcccaagatg tgaagcaaat cacccactta atgccgtgat
    70981 tgccagtaaa tcggcagcgg cggcaacagg cgaaaggtta atcgacagct caatttttac
    71041 actcatctcg tcggagatgt gacgcgacga aaaatgatga ggataagaag atgagtaacg
    71101 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    71161 cgccgttcaa aaaagtaaaa aagtggtcat gggaaaccat gtggtcagtc ggtgggattg
    71221 tttcgtggat tatcctgccg tgggccatca gcgccctgtt actaccgaat ttctgggcgt
    71281 attacagctc atttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    71341 ggatcggtaa tatcaactac ggcctgacca tgcgttatct cggcatgtcg atgggaattg
    71401 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    71461 atttcgatgt gctgattaat accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    71521 cgctgattgg cgtagggatt gtaactcgcg ccgggcagtt gaaagagcgc aagatgggca
    71581 ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
    71641 tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgccg
    71701 cgctgggcgt cgatcccctg tatgtcgctt tgccaagcta tgttgtcatc atgggcggcg
    71761 gcgcaatcat caacctcggc ttctgcttca ttcgtctggc caaagtgaaa gatttgtcgc
    71821 taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta ctctcagcac
    71881 tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    71941 cggcgcagta tgactacatc agctggatgc tgcatatgag cttctatgta ttgtgcggcg
    72001 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg acgccgtccg gtaacggtgt
    72061 tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc ggcatggcga
    72121 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    72181 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa atatcactga
    72241 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
    72301 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaaca cgctcactgc
    72361 atgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta gccgccagcc
    72421 gggtaatcag cttatccagc aatgtttcgc tggatgttgg cggcaacgaa tcactggtgt
    72481 aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc atttcgttag
    72541 caaacgacac atgctgacta ctttcatgct caagctgacc gataacctgc cgcgcctgcg
    72601 ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
    72661 tcgcgccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
    72721 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaata taaaagagat
    72781 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg cagaccatta ccgcgccaga
    72841 caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa gacatcttgc ggataacggt
    72901 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    72961 gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca atatggcgta
    73021 caaatacgtt gagaagattc gcgttattgc agaaaaccat cccgtccctg acgaatatca
    73081 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctgaat
    73141 ccgcagcgat aggcgatgtc agtaacgctg gcctcgctgt ggcgtagcag atgtcgggct
    73201 ttcatcagtc gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg ctgcttaagc
    73261 tgccgatgta gcgtacgcaa tgaaagagaa aattgagccg ccactgcatc ccaattcacc
    73321 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga tgcgctgttt
    73381 tccacgctct cctgcaaact gcttttacgc agtaagagca gtaattgcat aaacagaatt
    73441 tcacgactgg cggtcgaggg taaatcattt ccctcttctt gttgttccaa ctgtgcaacc
    73501 agttggcgca cctgctgtaa tacgcaatgg ttaacgcgcc agtgagacgg atactgccca
    73561 tcctgctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    73621 cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg ccgatcatga
    73681 tcgcgtacga aacaaaccgt cccaccggtg atggtatagg gctgcccatt aaatacatga
    73741 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    73801 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    73861 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    73921 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    73981 tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca caatgaagca
    74041 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    74101 taacgagaag gtcacgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    74161 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    74221 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tccatcgttt
    74281 taacaatggc ctgcatagcc agaacggtta tgtcacctgg aatgtggata gcctggaaag
    74341 tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg atagcattgg
    74401 gattgatacc tggggcgtgg actttgtgct gctcgaccag cacggtcagc gcgtgggtct
    74461 gcccgttgct taccgcgata gccgcaccaa tggcctaatg gcgcaggcac aacagcaact
    74521 cggcaaacgc gatatttatc aacgtagcgg catccagttt ttgcccttca atacgatcta
    74581 tcagctacgt gcactgacag agcagcaacc agaacttatt ccacacattg ctcacgcttt
    74641 gctgataccg gattacttta gttatcgcct gaccggcaag atgaactggg aatataccaa
    74701 cgccacgacc acgcaactgg tcaatatcaa tagcgacgac tgggacgagt cgctactggc
    74761 gtggagcggt gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcatagg
    74821 tcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
    74881 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
    74941 cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca cggcgctggc
    75001 agccaatatc accaacgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    75061 gggcttatgg ctgcttcagc gagtgcttca ggagcggcaa atcaacgatc ttccggcgct
    75121 tatcgccgca acacaggcac ttccggcctg ccgcttcatc atcaatccca atgacgatcg
    75181 ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    75241 accgatcccg gaaagtgatg ctgaactggc gcgctgtatt ttcgacagtc tggcgctgct
    75301 gtatgccgat gtgttgcatg agctggcgca gctgcgtggt gaagatttct cgcaactgca
    75361 tattgtcggc ggcggctgcc agaacacgct gctcaaccag ctgtgtgccg atgcctgcgg
    75421 tattcgggtg atcgccgggc ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt
    75481 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcactaccgc
    75541 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagat
    75601 tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg
    75661 gagcttgcga aacagcgttt cgcggcagta gggattgatg tcgaggaggc actgcgccaa
    75721 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
    75781 aacccggaag gttcgctgac cggtggtatt caggctactg gtaattatcc gggcaaagcg
    75841 cgtaatgcca gcgagctgcg tgccgatctg gaacaggcta tgcggctgat tccggggcca
    75901 aaacggctta atttgcatgc catttatctg gagtcagaca cgccagtctc acgtgaccaa
    75961 atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    76021 gattttaacc cctcctgttt ttcacatccg ctaagcgccg atggctttac actttcccat
    76081 gccgacgacc gcattcgcca gttctggatt gaccactgta aagccagccg ccgtgtttca
    76141 gcctattttg gtgagcaact cggcacaccg tcggtgatga acatctggat cccggatggc
    76201 atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtttgctggc agcactggat
    76261 gaagtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg
    76321 tttggcattg gcgcggagag ctacacggtt ggctccaatg agttttacct ggggtatgcc
    76381 accagccgcc agacggcgct gtgcctggac gccgggcatt tccacccgac tgaagtgatt
    76441 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagccgt
    76501 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcgattgcc
    76561 agtgagattg ttcgtcacga tctgctcgac cgggtgcata tcggcctcga cttcttcgat
    76621 gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg
    76681 ctgcgtgcgt tgctggaacc taccgctgag ctacgcaagc tggaagcggc gggcgattac
    76741 actgcgcgtc tggcgctact ggaagagcag aaatcgttgc cgtggcaggc ggtatgggaa
    76801 atgtattgtc agcgtcacga tacgccagcg ggtagcgaat ggctggagaa cgtgcggact
    76861 tatgagaaaa aaattttgag tcgccgcggg taaacactgc cggatgcggc acaagtgcct
    76921 tatccggcct acgggtcggc aatagttgta ggcctgataa gacgcgacag cgtcgcatca
    76981 ggcattgatt gccggatgcg gcacgagtgc cttatccggc ctacgggtca gcaacagttg
    77041 taggcctgat aagacgcgac agcgtcgcat caggcattga ttgccggatg tggcgcgagt
    77101 gccttatccg gcctacaggt cgtgcatccg acaacaccga attaacagga acacagaaca
    77161 tgcaaaacat aacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct
    77221 ggctgaaagg ctgggacgag cgcaacggcg gcaacctgac gctgcgcctg gatgacgccg
    77281 atatcgcacc atatcacgac aattttcacc aacaaccgcg ctatatcccg ctcagccagc
    77341 ccatgccttt actggcaaat acaccgttta ttgtcaccgg ctccggcaaa ttcttccgta
    77401 acgtccagct tgatcctgcg gctaacttag gcatcgtaaa agtcgacagc gacggcgcgg
    77461 gctaccacat tctttggggg ttaaccaacg aagccgttcc cacttctgaa cttccggctc
    77521 actttctctc ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc
    77581 actgccacgc caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct
    77641 tcactcgcca actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg
    77701 gcattctgcc gtggatggtg cccggcacgg acgaaatcgg ccaggcaact gcacaagaga
    77761 tgcaaaaaca ttcgctggtg ttgtggccct tccacggtgt gttcggcagc gggccgacgc
    77821 tggatgaaac cttcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg
    77881 tttattcgat gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggcc
    77941 agcgtttcgg cgttacacca ctcgccagtg cgctggcgct gtaaggagca aacatgatcc
    78001 gcaaagcctt tgtcatgcag gtaaaccccg acgcccacga agagtatcag cgtcggcata
    78061 atcccatctg gccggaactg gaagcagtgc tgaaatctca cggtgcgcat aactacgcca
    78121 tctatctcga caaagcgcgt aatctgctat ttgccacgat agagattgaa tctgaagagc
    78181 ggtggaatgc ggttgccagt acagatgttt gccaacgttg gtggaaatat atgaccgacg
    78241 ttatgcccgc taatgcggat aacagcccag tcagtagcga actgcaagaa gtgttttacc
    78301 tgccttaatg agttgcggtg ccggaaaata tccagcaccg ccctttcatc agaagaagta
    78361 tttaaagcga acgcgtgcgc cgtaacggtc atcactatcg tcgccgtgta tcttatcgcc
    78421 gtcgctatcc agaatggagt aatacgtgcc gaggtaaata ttaaagttct ccatatccat
    78481 cacattagcg aactgataag acgcatgaat ggtgtgaatg ttgtatgttc cttcatcatc
    78541 aatccagcaa tcgttatcac aaaccacgcc actaaattca tcaatcttat tatgtgcgta
    78601 gatataaccc agctcgaaac gtttccacag cgcgttaatc ccagcggtga aatctttctc
    78661 attattagca tctaaatagg cggtattaag attaaccacg atgccatttt ccggatcggt
    78721 tttcaggcca ttccaggtca tgctcatgcc ataaccggta cgatcggact gatcgacaaa
    78781 attacccttg ctatcggtat aaccataagc attatttacc acgttcgctt ccatcgctgc
    78841 ggaaacggtg aattcttccg tcggcgacca ggcaattacc ggacgcagat aagcaacatt
    78901 tttctgctgt tccatatcgc gtccatgata attaccgtcg ttatataaag atgttccgtc
    78961 ttccagtaac gtgtttaatt caaaatacca gttatcgagt tgtttgctga cgaggaaatt
    79021 accgccagcg ttagaacgcc cgcggccctc tttcatcata tagatataac cgctgccatc
    79081 gtcataaaga tcgttcgcag tattaccgga atgttcaaca aaggtatcct gattcagcgg
    79141 gaacatatcg taggcttcaa aacggcctac tttgaccttc cagtcgtttt ctttgccaaa
    79201 gaagaaaacc gcatcatcga tatttactga accgtgcata tcccccagcg gttgtgcgga
    79261 gaacccagcg aaatagccat tatccagttt tcgcatacca tcaaaaccta acagaatacg
    79321 accattgaga ttccactgtt cattagtggg atcgctattc acatcagcgt tggtcattgc
    79381 cagcaggcca tgattacttt ccgcatccat attaaattct acgtcaccgt agatttttaa
    79441 atcgccatac cctgaaagca ggagattagg cggggtggcg tttttcttct ctgactccgt
    79501 cgttgattga ccgtttaccg gcgtgttgcc ctgtgcggtt ttaatttcgc ggatctcggc
    79561 ggcttgttgt gtctgaagct gtttaacctg cccctctgct tttgctgccc gcatttctgc
    79621 cgcttccagg cgttcttcca gcaatgccat gcgttgttcc agcgttaaat gctgtgtttt
    79681 tgccataccg gtactacatg ccaataataa accgatggct aaagataatg tcgatttttt
    79741 cattatttac gttccctgaa ataaaggaga ccctacgctt tccaaaagga atacgtatat
    79801 agagaaatat aaggtgtgca ctttcgaatt tttaatccaa cttcagcagg ccgatggata
    79861 gtatcccgcc atataatatg agtaaatccg ctgatttcct ggaacgtgag gaatataaca
    79921 atagtgccgt ttttaa
//
LOCUS       DAPQZD010000004        70996 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPQZD010000004 DAPQZD010000000
VERSION     DAPQZD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.004"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(462..1094)
                     /locus_tag="RO454_000265"
     CDS             complement(462..1094)
                     /locus_tag="RO454_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDY0932155.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1389..1664
                     /locus_tag="RO454_000266"
     CDS             1389..1664
                     /locus_tag="RO454_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0932156.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
     gene            complement(1741..1983)
                     /locus_tag="RO454_000267"
     CDS             complement(1741..1983)
                     /locus_tag="RO454_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDY0932157.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2336..3256
                     /gene="lpxP"
                     /locus_tag="RO454_000268"
     CDS             2336..3256
                     /gene="lpxP"
                     /locus_tag="RO454_000268"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDY0932158.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3613..3684
                     /gene="ypdK"
                     /locus_tag="RO454_000269"
     CDS             3613..3684
                     /gene="ypdK"
                     /locus_tag="RO454_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDY0932159.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3749..4987)
                     /gene="alaC"
                     /locus_tag="RO454_000270"
     CDS             complement(3749..4987)
                     /gene="alaC"
                     /locus_tag="RO454_000270"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDY0932160.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5364..7061
                     /gene="ypdA"
                     /locus_tag="RO454_000271"
     CDS             5364..7061
                     /gene="ypdA"
                     /locus_tag="RO454_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDY0932161.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            7076..7810
                     /gene="ypdB"
                     /locus_tag="RO454_000272"
     CDS             7076..7810
                     /gene="ypdB"
                     /locus_tag="RO454_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDY0932162.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSCSKVKEFRQLMHL"
     gene            7823..8680
                     /locus_tag="RO454_000273"
     CDS             7823..8680
                     /locus_tag="RO454_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0932163.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(8683..11178)
                     /gene="ptsP"
                     /locus_tag="RO454_000274"
     CDS             complement(8683..11178)
                     /gene="ptsP"
                     /locus_tag="RO454_000274"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDY0932164.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(11203..12240)
                     /gene="ypdE"
                     /locus_tag="RO454_000275"
     CDS             complement(11203..12240)
                     /gene="ypdE"
                     /locus_tag="RO454_000275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDY0932165.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(12240..13325)
                     /gene="ypdF"
                     /locus_tag="RO454_000276"
     CDS             complement(12240..13325)
                     /gene="ypdF"
                     /locus_tag="RO454_000276"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDY0932166.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            complement(13340..14587)
                     /locus_tag="RO454_000277"
     CDS             complement(13340..14587)
                     /locus_tag="RO454_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDY0932167.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(14609..14935)
                     /locus_tag="RO454_000278"
     CDS             complement(14609..14935)
                     /locus_tag="RO454_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDY0932168.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(15154..16119)
                     /gene="glk"
                     /locus_tag="RO454_000279"
     CDS             complement(15154..16119)
                     /gene="glk"
                     /locus_tag="RO454_000279"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDY0932169.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            16323..17579
                     /locus_tag="RO454_000280"
     CDS             16323..17579
                     /locus_tag="RO454_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDY0932170.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            17694..18020
                     /locus_tag="RO454_000281"
     CDS             17694..18020
                     /locus_tag="RO454_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDY0932171.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(18161..19399)
                     /locus_tag="RO454_000282"
     CDS             complement(18161..19399)
                     /locus_tag="RO454_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDY0932172.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            19735..20937
                     /gene="nupC"
                     /locus_tag="RO454_000283"
     CDS             19735..20937
                     /gene="nupC"
                     /locus_tag="RO454_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDY0932173.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(20987..23176)
                     /locus_tag="RO454_000284"
     CDS             complement(20987..23176)
                     /locus_tag="RO454_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDY0932174.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(23383..23458)
                     /locus_tag="RO454_000285"
     tRNA            complement(23383..23458)
                     /locus_tag="RO454_000285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23423..23425),aa:Ala,seq:ggc)
     gene            complement(23498..23573)
                     /locus_tag="RO454_000286"
     tRNA            complement(23498..23573)
                     /locus_tag="RO454_000286"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23538..23540),aa:Ala,seq:ggc)
     gene            23794..24153
                     /locus_tag="RO454_000287"
     CDS             23794..24153
                     /locus_tag="RO454_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDY0932175.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            24155..24547
                     /locus_tag="RO454_000288"
     CDS             24155..24547
                     /locus_tag="RO454_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDY0932176.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(24598..26013)
                     /gene="gltX"
                     /locus_tag="RO454_000289"
     CDS             complement(24598..26013)
                     /gene="gltX"
                     /locus_tag="RO454_000289"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDY0932177.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            26272..26347
                     /locus_tag="RO454_000290"
     tRNA            26272..26347
                     /locus_tag="RO454_000290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26305..26307,aa:Val,seq:tac)
     gene            26392..26467
                     /locus_tag="RO454_000291"
     tRNA            26392..26467
                     /locus_tag="RO454_000291"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26425..26427,aa:Val,seq:tac)
     gene            26514..26589
                     /locus_tag="RO454_000292"
     tRNA            26514..26589
                     /locus_tag="RO454_000292"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26547..26549,aa:Val,seq:tac)
     gene            26594..26669
                     /locus_tag="RO454_000293"
     tRNA            26594..26669
                     /locus_tag="RO454_000293"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26627..26629,aa:Lys,seq:ttt)
     gene            complement(26780..27664)
                     /gene="xapR"
                     /locus_tag="RO454_000294"
     CDS             complement(26780..27664)
                     /gene="xapR"
                     /locus_tag="RO454_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDY0932178.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(27705..27863)
                     /locus_tag="RO454_000295"
     CDS             complement(27705..27863)
                     /locus_tag="RO454_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDY0932179.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(27916..29172)
                     /gene="xapB"
                     /locus_tag="RO454_000296"
     CDS             complement(27916..29172)
                     /gene="xapB"
                     /locus_tag="RO454_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDY0932180.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTNALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(29233..30066)
                     /gene="xapA"
                     /locus_tag="RO454_000297"
     CDS             complement(29233..30066)
                     /gene="xapA"
                     /locus_tag="RO454_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDY0932181.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            30315..31079
                     /locus_tag="RO454_000298"
     CDS             30315..31079
                     /locus_tag="RO454_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932182.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(31118..32044)
                     /locus_tag="RO454_000299"
     CDS             complement(31118..32044)
                     /locus_tag="RO454_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0932183.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            32134..33132
                     /locus_tag="RO454_000300"
     CDS             32134..33132
                     /locus_tag="RO454_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDY0932184.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(33129..33347)
                     /locus_tag="RO454_000301"
     CDS             complement(33129..33347)
                     /locus_tag="RO454_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDY0932185.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(33349..35364)
                     /gene="ligA"
                     /locus_tag="RO454_000302"
     CDS             complement(33349..35364)
                     /gene="ligA"
                     /locus_tag="RO454_000302"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDY0932186.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(35435..36433)
                     /gene="zipA"
                     /locus_tag="RO454_000303"
     CDS             complement(35435..36433)
                     /gene="zipA"
                     /locus_tag="RO454_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDY0932187.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            36663..37424
                     /gene="cysZ"
                     /locus_tag="RO454_000304"
     CDS             36663..37424
                     /gene="cysZ"
                     /locus_tag="RO454_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDY0932188.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            37609..38580
                     /gene="cysK"
                     /locus_tag="RO454_000305"
     CDS             37609..38580
                     /gene="cysK"
                     /locus_tag="RO454_000305"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDY0932189.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            38964..39221
                     /gene="ptsH"
                     /locus_tag="RO454_000306"
     CDS             38964..39221
                     /gene="ptsH"
                     /locus_tag="RO454_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDY0932190.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            39266..40993
                     /gene="ptsI"
                     /locus_tag="RO454_000307"
     CDS             39266..40993
                     /gene="ptsI"
                     /locus_tag="RO454_000307"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDY0932191.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            41034..41543
                     /gene="crr"
                     /locus_tag="RO454_000308"
     CDS             41034..41543
                     /gene="crr"
                     /locus_tag="RO454_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDY0932192.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(41586..42437)
                     /gene="pdxK"
                     /locus_tag="RO454_000309"
                     /gene_synonym="thiJ"
     CDS             complement(41586..42437)
                     /gene="pdxK"
                     /locus_tag="RO454_000309"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDY0932193.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            42542..42910
                     /locus_tag="RO454_000310"
     CDS             42542..42910
                     /locus_tag="RO454_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDY0932194.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(42913..43824)
                     /gene="cysM"
                     /locus_tag="RO454_000311"
     CDS             complement(42913..43824)
                     /gene="cysM"
                     /locus_tag="RO454_000311"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDY0932195.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(43959..45056)
                     /gene="cysA"
                     /locus_tag="RO454_000312"
     CDS             complement(43959..45056)
                     /gene="cysA"
                     /locus_tag="RO454_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDY0932196.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(45046..45921)
                     /gene="cysW"
                     /locus_tag="RO454_000313"
     CDS             complement(45046..45921)
                     /gene="cysW"
                     /locus_tag="RO454_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDY0932197.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(45921..46754)
                     /gene="cysT"
                     /locus_tag="RO454_000314"
     CDS             complement(45921..46754)
                     /gene="cysT"
                     /locus_tag="RO454_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDY0932198.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(46754..47770)
                     /gene="cysP"
                     /locus_tag="RO454_000315"
     CDS             complement(46754..47770)
                     /gene="cysP"
                     /locus_tag="RO454_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDY0932199.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(47928..48719)
                     /gene="ucpA"
                     /locus_tag="RO454_000316"
     CDS             complement(47928..48719)
                     /gene="ucpA"
                     /locus_tag="RO454_000316"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDY0932200.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            48999..49895
                     /gene="murQ"
                     /locus_tag="RO454_000317"
     CDS             48999..49895
                     /gene="murQ"
                     /locus_tag="RO454_000317"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDY0932201.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            49899..51323
                     /gene="murP"
                     /locus_tag="RO454_000318"
     CDS             49899..51323
                     /gene="murP"
                     /locus_tag="RO454_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDY0932202.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(51501..52400)
                     /gene="yfeX"
                     /locus_tag="RO454_000319"
     CDS             complement(51501..52400)
                     /gene="yfeX"
                     /locus_tag="RO454_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDY0932203.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(52496..53071)
                     /locus_tag="RO454_000320"
     CDS             complement(52496..53071)
                     /locus_tag="RO454_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDY0932204.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(53132..53581)
                     /locus_tag="RO454_000321"
     CDS             complement(53132..53581)
                     /locus_tag="RO454_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDY0932205.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(53568..53993)
                     /locus_tag="RO454_000322"
     CDS             complement(53568..53993)
                     /locus_tag="RO454_000322"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDY0932206.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            54207..55076
                     /gene="amiA"
                     /locus_tag="RO454_000323"
     CDS             54207..55076
                     /gene="amiA"
                     /locus_tag="RO454_000323"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDY0932207.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            55080..55979
                     /gene="hemF"
                     /locus_tag="RO454_000324"
     CDS             55080..55979
                     /gene="hemF"
                     /locus_tag="RO454_000324"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDY0932208.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(55985..57037)
                     /gene="eutR"
                     /locus_tag="RO454_000325"
     CDS             complement(55985..57037)
                     /gene="eutR"
                     /locus_tag="RO454_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDY0932209.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(57083..57583)
                     /gene="eutK"
                     /locus_tag="RO454_000326"
     CDS             complement(57083..57583)
                     /gene="eutK"
                     /locus_tag="RO454_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDY0932210.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(57596..58255)
                     /gene="eutL"
                     /locus_tag="RO454_000327"
     CDS             complement(57596..58255)
                     /gene="eutL"
                     /locus_tag="RO454_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDY0932211.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(58265..59152)
                     /gene="eutC"
                     /locus_tag="RO454_000328"
     CDS             complement(58265..59152)
                     /gene="eutC"
                     /locus_tag="RO454_000328"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDY0932212.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(59173..60534)
                     /gene="eutB"
                     /locus_tag="RO454_000329"
     CDS             complement(59173..60534)
                     /gene="eutB"
                     /locus_tag="RO454_000329"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDY0932213.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCILAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(60546..61949)
                     /gene="eutA"
                     /locus_tag="RO454_000330"
     CDS             complement(60546..61949)
                     /gene="eutA"
                     /locus_tag="RO454_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDY0932214.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(61946..63172)
                     /gene="eutH"
                     /locus_tag="RO454_000331"
     CDS             complement(61946..63172)
                     /gene="eutH"
                     /locus_tag="RO454_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDY0932215.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(63389..64576)
                     /gene="eutG"
                     /locus_tag="RO454_000332"
     CDS             complement(63389..64576)
                     /gene="eutG"
                     /locus_tag="RO454_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDY0932216.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(64566..65402)
                     /gene="eutJ"
                     /locus_tag="RO454_000333"
     CDS             complement(64566..65402)
                     /gene="eutJ"
                     /locus_tag="RO454_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDY0932217.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(65413..66816)
                     /locus_tag="RO454_000334"
     CDS             complement(65413..66816)
                     /locus_tag="RO454_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDY0932218.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGPTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(66828..67115)
                     /gene="eutN"
                     /locus_tag="RO454_000335"
     CDS             complement(66828..67115)
                     /gene="eutN"
                     /locus_tag="RO454_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDY0932219.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(67222..67515)
                     /gene="eutM"
                     /locus_tag="RO454_000336"
     CDS             complement(67222..67515)
                     /gene="eutM"
                     /locus_tag="RO454_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDY0932220.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(67554..68570)
                     /gene="pta"
                     /locus_tag="RO454_000337"
     CDS             complement(67554..68570)
                     /gene="pta"
                     /locus_tag="RO454_000337"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDY0932221.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(68567..69370)
                     /gene="eutT"
                     /locus_tag="RO454_000338"
     CDS             complement(68567..69370)
                     /gene="eutT"
                     /locus_tag="RO454_000338"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDY0932222.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(69367..70068)
                     /gene="eutQ"
                     /locus_tag="RO454_000339"
     CDS             complement(69367..70068)
                     /gene="eutQ"
                     /locus_tag="RO454_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDY0932223.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(70043..70522)
                     /gene="eutP"
                     /locus_tag="RO454_000340"
     CDS             complement(70043..70522)
                     /gene="eutP"
                     /locus_tag="RO454_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDY0932224.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(70535..70870)
                     /gene="eutS"
                     /locus_tag="RO454_000341"
     CDS             complement(70535..70870)
                     /gene="eutS"
                     /locus_tag="RO454_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDY0932225.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        17120 a        19349 c        17678 g        16849 t
ORIGIN      
        1 ggcataacgc ctctataaat aatctgcaaa atatcgtcgt agctgcactc gatattttta
       61 aattcctgta tcaagtgctg aaaatcattt catcgctact tccaattcct ttctgcatgg
      121 taagtagtga ggacgaaaag aagaatataa taaatattgg ttcgcggtaa tcagagagag
      181 ccgcgacatt tgtgatgtta ttcttacaac tgtaagaagt gtcagtgatt tacaagtaag
      241 gttatgatta cactatttaa agcgtaccat aaaatataac ataataaatg atttgtgata
      301 atatgggatt attgcggttt actttgagga agataaaatg cgagtatata gaaccgcgag
      361 cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg
      421 agggagttaa atcctttacc tcaatatatt gcctgtcagg tttagttcac aaacagtgct
      481 tcgctatcaa cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat
      541 tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttgagcggc
      601 atcagatatt ggttttccat tacgccaaca ccggccaggc gcagctcttc cattgcgccc
      661 tttttatccc ctttcgccat tttttcgttg gcaattttta tcgcggcttc tttttcgggt
      721 gtcgctacat aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca
      781 ttgacgttgg tctttttacc tggcttagcg aactttgccc attcggtact atcatctgac
      841 agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga
      901 gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct
      961 gctgctgccg ttttctgggt ttgcttaact tgctgagttt gctgttgtgc cgctaccggt
     1021 gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata
     1081 attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt
     1141 gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacaa
     1201 ctgtagttat tatcgcaaag ccgcttttag tcttacagtt gtaggaatct gcttcctgtc
     1261 gcagagaact attcttatat accacaaaga tatgtcattt attatatcga tgtaaatact
     1321 gattaattaa atataaatac agccataaca tcgtaggttt tcagtgaacc atttccagga
     1381 tattttctat gaaagtaaac ttaatgcttt ttagcttatt tttatttgtc tctattatgg
     1441 catgcaatgt ttttgcattt tctatttctg gtggtggtag tgaggcgagc tataaagaga
     1501 cagaaaaaac gtcagtgatg acgacaacgc attctacaaa acttcagcca tcacaggcga
     1561 ttttgtttaa gatgagagaa gatgcaccac cattaaacct cacagaagac atcacgccta
     1621 cttatccgac aaaggcgaat tatctcattc atcctttgcg atagcgtctg tcacctggca
     1681 ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca
     1741 ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa
     1801 cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga agtaataaat
     1861 gagtgcagcg aggatcacca tgccgaagaa agaagagatc gccgatacca tgttcagtac
     1921 ctgaccgatg tatccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat
     1981 catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga
     2041 caatccttaa cctctggcaa tttttgctaa gcatcaatca aaacagcatg ttaaatgcaa
     2101 gactgttacg tacggaaaaa tatttacttt gcacgattaa taatcagtag ctgaaagcag
     2161 tcagcgagtg ataaagtaca gataccgcat tcatgctcct cgtggttatg tcctgactag
     2221 tctttacact ctttacagga accattgtcg tacatgatgg cctaatcaat tgagtgtacc
     2281 gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt
     2341 tccaaaatgc aaattttccc gcgagtttct gcatcctcgc tactggctca cgtggtttgg
     2401 gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac
     2461 gcgtatcggc acaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa
     2521 cctggaactt tgtttcccgc agcattctgc ggaagagcgc gagaagatga ttgccgaaaa
     2581 ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcgtggttct ggcccgacag
     2641 tcgcgtacgt aaatggtttg atgtggaagg gctggataac cttaaacgcg cacaaatgca
     2701 aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggtcgggt
     2761 gatgggactt tgccaaccaa tgatggccac ctatcgcccg cataataatc agctgatgga
     2821 atgggtccag acccgtggtc gcatgcgctc taacaaagcg atgatcggca gaaataatct
     2881 gcgcggcatt gtcagtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga
     2941 ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac
     3001 caatggcacc tatgtcctct ctcgtctctc tggcgcagcc atgttgaccg taacgatggt
     3061 aagaaaagcg gattacagcg gatatcgttt gtacatcacc ccagagatgg aaggctaccc
     3121 gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg
     3181 cgcaccggag cagtacctct ggatacaccg tcgctttaaa acgcgcccgg tgggtgaatc
     3241 gtcgttatac atttaacaaa cggctctcac tctttccctc ctcaaatcag ccataataac
     3301 ctcatggttt ttatggcttt ttttcttaag gttttttcct tcgctgaata tctaattatt
     3361 accgtcagaa attaaactgt cgccgatatg agacacctgt aagttactaa aagtaaataa
     3421 gaaaatgcct cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt
     3481 agctcagaat tctgggatga tgcccacggt aaaatacagg acgtttaagg tcgttttgcc
     3541 cggtgtcaac tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg
     3601 gagagatgta aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc
     3661 ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccattagg
     3721 ctcctgtttg catttctggc gaaggcgttt attccgcgtt ttcgtgaata tgtttgctgc
     3781 tggcgggtag taaaccgtcg gcacggaaca tcgctttaat cccacgaatc gcctgacgaa
     3841 tacggtcgcg gttttcaatc agtgcaaagc gcacatgcgt gtcgccgtag tcgccaaagc
     3901 caatccctgg agagacacag accttcgctt cgttaagcaa tttcttggca aattccagcg
     3961 agcccatggc agcatacggt tccgggattt tcgcccagac atacatcgaa gccttaggca
     4021 tttcgaccat ccagcccgct tcatgcagcc ctttcaccag cacatcgcgg cggcgtttgt
     4081 actgttcggc aatatcgcgc acgcactgtt gatcgccctc cagcgccgca atagccgcca
     4141 cctgcaacgg cgtaaaggtg ccgtaatcgt gatagctttt aatacgtgcc aacgcgctga
     4201 ccagcgtttt gttaccgacc ataaagccga tacgccagcc cgccatgttg tagcttttcg
     4261 acagcgtaaa gaactcgacc gccacgtcgc gtgcgcccgg cacctgcatg attgacggcg
     4321 ctttccagcc atcgtagacg atatcggcat aggccaggtc gtggaccacc agcacatcgt
     4381 agcgtttagc cagcgccaca accttctcaa agaattccag ttccacgcat tgcgcggttg
     4441 ggtttgacgg gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggcgc
     4501 gctccagttc attgaagaag tcgacacctt ccaccagcgg cactgagcgt acctgcgccc
     4561 cggcaatcac cgcgccgtaa atatgaatgg ggtagcttgg attcggcacc agcactgtgt
     4621 caccatgatc cagcgtcgcc agcatcaggt gcgccagccc ctctttcgaa ccaatagtga
     4681 cgatggcttc tgattccggg tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa
     4741 tggcgcgacg taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct
     4801 gggccaccgt acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca
     4861 tactgaaatc gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtaa
     4921 tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt
     4981 cagccataat ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacga
     5041 tggtggctgt tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt
     5101 ttatcaatgg ctttaaatga ggaactccac ttcaaaaaca ggaaatttat ctacgctaac
     5161 aagaaaatta ccactcattt tattaacatt ataatttcaa taaatttacc aacaagatgt
     5221 gcattatcaa gagatgcatt gctaatacac catttatctt tcaatcccct tttaaaattg
     5281 tggtttttcc tcaaaaaagg cctgctggtc atcagccttc aggttaccta tcatagaggt
     5341 ttaatcctta ttcagagtca cccgtgcacg aaatattcaa catgctgctg gcggtcttcg
     5401 atcgggcagc gttaatgctt atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg
     5461 aactgttgca caagtcggcg cactccccaa aagagttgct cgccgttacc gccattttct
     5521 cgctgttcgc cctgttcagc acctggtccg gcgttcccgt agaaggctcg ctggtgaacg
     5581 tacgtattat cgcggtgatg tcaggcggga ttctgtttgg cccgtgggtg ggcatcatta
     5641 ccggcgtgat tgcgggtatt caccgttatt taattgacat cggcggcgtg acggcgatcc
     5701 cctgctttat caccagcatt ctggcgggtt gtatatcggg ctggatcaac ctgaaaatcc
     5761 ccaaagcaca gcgctggcgc gtcggtattc tcggcggcat gttgtgtgaa acgttgacca
     5821 tgattctggt gattgtctgg gcaccaacta ccgcattggg gatcgatatc gtctctaaaa
     5881 tcgggattcc aatgatcctc ggcagcgtct gtatcggctt tattgtgttg ctggtacaaa
     5941 gtgttgaggg agaaaaagag gccagcgccg cgcggcaggc caagctggcg ctggatatcg
     6001 ccaacaaaac gctgccgctg tttcgccatg tcaatagcga gtcattacgc aaggtctgcg
     6061 aaattatccg cgatgacatt cacgccgatg cggtggcgat gactaatacc gatcatgtgc
     6121 tggcctacgt tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga
     6181 ctacccgtca ggcgatgaat tacgggaaaa tcatcattaa aaacaatgat gaagcccacc
     6241 gcacaccaga gattcattcc atgctggtga tcccattgtg ggagaaaggc gttgtgaccg
     6301 gaacgctgaa aatttactac tgccacgcgc atcagataac ctcgtcatta caggaaatgg
     6361 cggtcggtct gtcacaaatc atctccacgc aacttgaggt gtcacgcgcc gagcagctac
     6421 gtgaaatggc aaataaggca gagcttcgcg ccctgcaaag caaaattaat ccccatttcc
     6481 tgtttaacgc tttgaacgct atttcatcgt caatccgtct gaatccggat actgctcgcc
     6541 agctgatctt taatttgtcg cgctatctgc gctataacat tgaattaaaa gacgatgagc
     6601 aaatcgatat caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc
     6661 gctttggcga caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc
     6721 ccagcctgct gatccagccg ttggtggaaa acgccattgt ccacggtatc cagccttgca
     6781 aaggtaaagg cgttgtcacc atcagcgttg ctgagtgcgg taatcgggta cgcattgcgg
     6841 tgcgagatac cgggcacggc atcgatccaa aggtgattga gcgggtcgaa gcgaacgaaa
     6901 tgccgggcaa taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg
     6961 aggggctgca tatccgccgc ctggaaccag gaacggaaat tgcgttttac attcctaacc
     7021 aacgcacccc agtcgcctca caggctacct tattgctttg agccggagtg atattgtgaa
     7081 agtcatcatt gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga
     7141 gcacagccag atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt
     7201 gcagcataac cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggtgt
     7261 gttgctggca caaaacatca gccagttcgc ccataaaccg tttattgtgt ttatcaccgc
     7321 gtggaaagaa catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc
     7381 gtatcaggag tcacgcatta ccgggatgct gcaaaaactg gaagcagcct ggcaacaaca
     7441 gcagaccagc agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt
     7501 gaaagatgag cgaatcatcg tcacgcccat taacgatatc tattacgccg aagcgcatga
     7561 gaaaatgacc tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga
     7621 gttttgcagc aagctgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa
     7681 tctgaacaaa atccgcgaga tcgaaccgtg gtttaataac acctatattc tgcggctgaa
     7741 agatctggat tttgaagtgc cggtcagctg cagcaaagtt aaagaattcc gccagttaat
     7801 gcatctttaa tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt
     7861 cgccgatctg ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc
     7921 cagccagcct acctcattgc cagtgggcag tttatgtatt gattttcccc gtctggatat
     7981 cgtgctacgc ggcgaatacg gcaatctgct ggaagcaaag cagcaacgta tggtggaagg
     8041 agaaatgctg tttattccag cacgcgcggc taatttaccg gtcaacaaca aaccggtgct
     8101 gctgttaagc ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac
     8161 cacgtcgttg ttacatcctg ctcgccagat ccagcttccc agcctgcaac gcggtgaagg
     8221 tgaagcgatg cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat
     8281 tcagccactg gtgttaagtt tgctgcatct ttgccgcaac gtggtgaata tgccgccggg
     8341 caattcgcaa ccgcgcggcg attttctcta tcacagcatt tgtaactgga ttcaggataa
     8401 ttatgcccag ctgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgccaaatca
     8461 tctgtcaaaa ctgtttgctc agcatggaac aatgcgtttt atcgagtatg ttcgctgggt
     8521 acgaatggca aaagcgagga tgattttgca aaaatatcat ctgtcgattc atgaagtggc
     8581 gcagcgttgc gggtttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttgg
     8641 tctgacgccg ggagagtata gcgcacgttt tcagggctaa cgtcagaagg ttaattccgt
     8701 ttccagcagc gtcagaatac tttgtgcatc ttgcgcggca aacagcgact ggcggaagtt
     8761 tttattcacc agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt
     8821 agcgcccagc gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaac
     8881 cggtttcacc aaccgggcaa tgctgatact ggaatgacgg atccactgtg atttggtgtg
     8941 cgggatcgcc acgccaaaac caacgccagt agtaacaatc tcttcccgct gccagacatc
     9001 ttcttccagc tcgaacggat gttcagtacg cccgttaaca ccgaggttac cgcacaggaa
     9061 ctggatcgcc tgctctttat tactaaagga ctgatcaaca aagatattct ctagtgccag
     9121 cagtgggcga acgtcttctt ccggcgcaaa cgcggtaagt aacgcttcaa tttcctgcgc
     9181 gctgcggcac tcgcacgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg
     9241 aagctggctt ttcactgccg gaatacgcgg gctactcatg cttagctcat ccagccccag
     9301 ccctagcagt agcggcagat aacgactttc accgcccagt tcaccgcaaa tacctaccca
     9361 tttgccgcgc tgatgcgcag cggtaacaat ctgctgcaac atgcgcagga acgatggcgt
     9421 aatcgggtta tacagcggcg atacgcgcgg gttattacga tcgactgcat acagatactg
     9481 ggtcatatcg ttagaaccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat
     9541 gtagcacacc gacggcactt ccaccatgat acccagcgta atcgtctctg catgacgcag
     9601 gccatcgcgc ttaagctcaa cgatcgcttt ttggatctca cccttcaccc ataagatctg
     9661 atcgagactg tggaccattg ggatcatcaa ctgggcgttg ccgaaactgg cagcgcgcaa
     9721 aatcgcccgc aattgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg
     9781 atagccgagg aacgggttct cttcctgggg aatgttgagg taaggaatgc ttttatcgcc
     9841 accgatgtcc atcgtgcgga agataatcgg cttgtcgccc gccgccaata gcacctgctg
     9901 gtacgcttca aactgttcct gctcgtccgg cgcgctgtcg cgatccatat agagcatttc
     9961 ggtacgaaac agcccaacgc cttccgcgcc gttagcaaac gcgcctgggg cttccagagc
    10021 ggtgccaata ttcgccgcaa tatcaatacg tttgttatca cgggaatagg ccagctgcgc
    10081 ggcagcttgc gcctgttgtt tttggcgttt atccgccagc gtctgcgcga cctgataata
    10141 accgctcacc gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcactgc
    10201 aggttgcccg gcataacggg caatcgcatc cagcggcagt ccgctcagta ccgggatcgc
    10261 cgaagcgcgg gccaggatca gggtatgcga ggtgcgcccg gttttctcca gaatcatgcc
    10321 cgcaagattt tttaaatcga ggctcaaaaa ctggcttggg gttaaatctt cagccaccag
    10381 aatggtcagt ttttcaagca ccagattatt gcgcggtttt agttccggcc aggtgatatg
    10441 cagcaactgt tcgctgatat cgcgaatgtc gctgacccgc tcgcgcagat aatcgctggc
    10501 agaagcagaa agtttggcac aaacctgctc catattgctg atgatcgccg cccccagccc
    10561 ctgatgctgt tctgccatca ggtgacggat attgcctgca aattcatcat cctgaatcag
    10621 cgacaaatgg gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctggtt
    10681 cagttgctcg gcaagcgttg ccagactgtg ctccagtagg gtggaatctt gcgcactcgc
    10741 agggatcacc cgataactgt cgaggctgtc gctctgtaac agggtcagtg tacccacgcc
    10801 aacgccgctt gccagcacgt tgccgtacag taaatccggg ttcaggcggc ttaatgaacg
    10861 cggcagcgga tgcgccgtca gttctgccag cgtcggctgg acgctatcgc tgtcgataaa
    10921 gcgcacctgg atatactctt ccagcactcg ccgcgcctgc tcttcatcgc taccgctaat
    10981 gttcaggctg caactgtcat taaataaagt gccagtgcca atcagcgcca gcgagctttt
    11041 cgcatcggcc tttgcgttct ggcgatggtt aataaaagtg atttcgcttt gccactggct
    11101 gcactgctct ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag
    11161 aaattgaatc gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag
    11221 ttgaacaacc gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg
    11281 caaaatgtca cggcagtcag caatcgatgc ggcgcaatgt ccgtggcggg ttgcaggccc
    11341 catcaccact gtgggtacgc cagtgccggt taagtgcacc gccccaccgt ccgtgccgcc
    11401 attgctgaac atatctgact gcaacggtac gccaatttct gccgccacgg tttcgatcca
    11461 ggcggtaagt tttggcggcg caatcagtga cttgtcgctt aacaccagca tcggcccgtt
    11521 accaatctgg cgatggttag ctgcgccata atcaaagttt ttcgcccagc aggcagtatc
    11581 aagcacaatg gcgacatccg gcgacaccgc gcgggtggca gtttgcccgc cgcgtaatcc
    11641 cacctcttcg ctggaacttg ccaccagcca cacttccgca ggcagttcgg cgtcgtgcag
    11701 ctcacgcagt aacgtcacca gcagatagca accgaggcgg tcatcaaagg ctttccccat
    11761 cacccgctgg tgagggagaa cctgaaaagt ggtatcgaag gtgacgcgat cgcctggacg
    11821 aattcctgcc tgcatcactt cgtcacagga gcgcgcgcca atgtccacgc gcatggcgct
    11881 gacgtcattc ccctgccggt cgctgtcaag caggcctgga attttgcact cttcacgggt
    11941 agtgatacgc accgactgaa gctggcgggc agccatgcgt acattgccaa ccggcaacac
    12001 atcgatcgcc ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgcgc
    12061 acagatcatc accttcggac ctgtcgactc attgagtcgg atcagcaccg atcccaggcc
    12121 gtcaaatcgc acttcttttt gcaggcgatc cgcttcttcc cgcaggatct gccgtacttc
    12181 ctgttctgag gaggcgatcg catctgcctc gctcaacgct tttaagagcg ataaatccat
    12241 tatgcctctc ccgtgagcaa cactgttttc ggcacggcgt agagcacttc tgcgccttgc
    12301 ggggtgacca gcacgacatc ttcgatgcgc acgccccctt gccctggcaa ataaatccct
    12361 ggctccacgg tcagtaacat acctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc
    12421 ggttcttcat ggacttcaat accgatagca tgaccggtgt tatggccgaa aaacttgcca
    12481 taaccggcct cggtaatcac gcggcgcgcg gcatcgtcaa cctgctggca gcgcacgccg
    12541 gggcgaatta cagagattgc cgcgagctgt gcctgcagga caatctgata gacgttaaac
    12601 agcggatgag attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca
    12661 gagcagtagc cctgatacag cgcaccgaaa tcgagagtga caaactcgcc cgctgcaaca
    12721 atcttgtcgc tggctttgcc gtgcggcagc gccccacgcc agccgctggc gacaatggtg
    12781 tcaaaagagg ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc
    12841 tcgcattcgc tcattcccgc ctgaataaag cggcgaatat gctctgcgcc acgatcggca
    12901 atcccgcagg caaggcggat tttctccacc tcttctggcg ttttgatttg ccgcagcaca
    12961 tccggcgtgg cgctcaccag tttcgcattg agttcggact gccagcggtg cgcggtttcc
    13021 caactcacct gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttgt
    13081 ctgacgatgg tggcaagcgt gtgcatcgcg tcgagcaaat gcagctggta accttgcgtg
    13141 cgggcttcta catccgcgta atagcgcgaa tccaccagaa tgtgcgcact ttcacggcta
    13201 atcaccacat aacctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag
    13261 gagagaagca ccgcatccag ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat
    13321 gtcatcagga ttgtccttct tacaggctat cgattaacaa tttgccttta cggaacatca
    13381 tcagacgcag gaacaccacc atcagcgcag ttatgactgc ccccagcgcg atccccgcca
    13441 tgtagacgcc gaggttagtg accagcggcc atgcccagat agcggactcc gggaaccatt
    13501 gcactgcgcc cagccagaca gcggcggttg agccgacaat cgcgccgacc atatacgacg
    13561 gaatggcggt gatagggctt tccagtgcaa acggaatcgc accctcactg atccccataa
    13621 acgccaggaa catggcggtt ttaccctgcg gataaagctg ggcgttgaac aagcgtttgc
    13681 cggttaaacg acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag
    13741 cgatagaacg tgcagtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac
    13801 ctgccgcttt gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg
    13861 cgtacatcag tgcgcccttc tcacctgcag cggtcagcac ggtacggata ccgccgttga
    13921 tccagccacc aaagggggtg atgacgtagt acatcgccag catgacaaaa atggcggaaa
    13981 gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct
    14041 ggttcatcca tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg
    14101 tggacggcac cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt
    14161 gcgtcggctg ggtagacatc aagccgccga taaaaccagc cgggaacgcc agtttgccac
    14221 cgatagagtt cgccacaaaa gcagcaaaca tcggaattgc aaaaccaaac aacacgccgc
    14281 cgaaggactg cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc
    14341 cgctattaag agcatccatg atgccgatat cagcaggaat tttaagccag ctataagcaa
    14401 tcagctgact gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa
    14461 tccctgacat cacatgctgc gggagttcac cccagaagct ggatttagag gcctgcggat
    14521 tcttaactgc cgctgccgca ccggatgcgc catgcacaac ggttgcactg cgttttttaa
    14581 tggccataac aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga
    14641 tttctttgat gattcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt
    14701 cgcgtgattc gaaacgctcg ttatcttccg gggtaactgc cacggagtgg atgatgatgg
    14761 tcgcttcggc gatatcctgc gccgtcaggc gattctggat accgtccgcg ccctgggttt
    14821 caattttcac ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa
    14881 aagtgtgagc caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta
    14941 attcctcgtt cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt
    15001 accagacaaa taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg
    15061 tcggccttgt gaggggacac aaagaaaaat ttaattaact gtatgattta aaagattatc
    15121 gggagagtta cctcccgata taacaggaag gatttagaga atgtgaccta aggtctggcg
    15181 taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat ataccggaat
    15241 atcatggaca tattccttaa agcgcccttt atcttcaaat gcggcgcgga aaccagaggc
    15301 tttgaagaac tcaagaaagc gcggcacgat accacccgca ataaacacgc cgccaaatgt
    15361 cccgagattg agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa
    15421 cgcgcggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt
    15481 gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat tcaccagccc
    15541 agggccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca atatttcgag
    15601 gataatgccc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt ctcctggcaa
    15661 gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg ttccggcacc
    15721 gtaaaccgca ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc
    15781 ttttttcagc atcgggatcg ccatcgacac agcagtaaaa tcgttaataa tttccagatg
    15841 gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat
    15901 tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac
    15961 cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga
    16021 ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt
    16081 ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag
    16141 tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg
    16201 caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt
    16261 gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga
    16321 acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg
    16381 ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt
    16441 tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct ctctggatca
    16501 tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc
    16561 atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc
    16621 taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg
    16681 aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac
    16741 cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt
    16801 ttggcacacc tgttgcggcg gcattgatat tttcgcaaac cttaaatggc agtaatgaag
    16861 ttccgctatg ggatcgtctc tttgcgccgt taatggcggc agcagctggt gcacttacta
    16921 ccggattatt tttccatcct catttttcac tgcccattgc gcattacgga cagatggaaa
    16981 tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg
    17041 ccgtatggtg cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat ccggtgttcg
    17101 tgctgggtat tggcggattt attctcggta ttctgggggt tattggtgga ccggtttcgc
    17161 tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg
    17221 attacttttt gctggcggtg ataaaacttg ccgccctggt cgttgctgcc gccagtggct
    17281 ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc
    17341 atgagcacgt acccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg
    17401 tgctggtggt aacacgtgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca
    17461 ataccacatt gctaccgctg ctctgtatcg tcatgctccc ggcatggctg ttattagcag
    17521 gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat aacgtttagc
    17581 aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggcgct ttacctcccc
    17641 gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca
    17701 ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcggca aacgcaggtg
    17761 aaatcaccct actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt
    17821 actgggacgg tggtcactgg cgcgaccgtg actactggca ccgcaattat gaatggcgta
    17881 aaaaccgctg gtggcgtcat gataatggct accatcgtgg ctgggataag cgtaaagcgt
    17941 atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgtgga aaaggccgcg
    18001 gacatggaca ccgccattaa acacattcgc caatggagca caatgcctga tgcgacgcta
    18061 gcgcgtctta tcaggcctac aaaccagctc tttaacgcgt agggcggata aagcatttac
    18121 gccgcatccg ccaatggtgc caaatgcgac gctcattcaa ctacaatccc agtgccgttc
    18181 ctaccagtaa ccagatattc agcgccacta ccagcaccac aatcacccag cctgtctgtt
    18241 ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcaaca
    18301 gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc
    18361 gtgtcggatc taatcccatc agaatgacaa taaatgacgg cagcatggtg acagtacgac
    18421 gcacccacag cgggatatga aagcgaatga agccctgcat cactacctgc cccgccagcg
    18481 tccccaccac cgttgaggac aacccggcag caaccaggct taatccaaag accgttgccg
    18541 cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag
    18601 tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga ttaacaaaac
    18661 cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg
    18721 aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg
    18781 tcgcccctaa cacgcccgcg gccaggaaga ccgcctccga agtaggtaaa ctcgggatca
    18841 ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag
    18901 cagcggcaac aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac
    18961 gttgcagcat taaaataagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg
    19021 aaacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg
    19081 ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat
    19141 cgcgaatctg ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggattt
    19201 gaatcagcat cgccatcagg ttagcccaaa cgacaaccca cagtagctga tagccgaagc
    19261 tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa
    19321 tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct
    19381 caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta
    19441 cgttaaatga gaatgattat caaattcatt taaatgggtt gtgatgattt ctctgataga
    19501 ccaggattat gactacgaaa agatagatga ctcgaatgtt atggttatgt ttaatattag
    19561 cacatttaca taaccttccg cttccataca caacatagca gaaatgtatg acagatcact
    19621 atttttgaag cttgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac
    19681 catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac
    19741 cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc
    19801 agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta
    19861 ctggcgtggt tcttcctgaa ctccgacgtt ggtctgggct tcgtgaaagg cttctccgaa
    19921 atgttcgaaa aactgctcgg ttttgccaac gaagggacta acttcgtctt tggtagcatg
    19981 aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct
    20041 gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt
    20101 ttcctgctct ccaaagtcaa cggcatgggc aaactggaat cctttaacgc cgtcagctcc
    20161 ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg caaaatctcc
    20221 cgcaatcgta tgtacaccat ggcagcaacg gcgatgtcca ccgtgtcgat gtccatcgtt
    20281 ggtgcataca tgaccatgct ggagccgaaa tacgtcgttg cggcgctggt actgaacatg
    20341 ttcagcacct ttatcgtgct gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa
    20401 aatattcaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac
    20461 attctggcag gtttcaaggt tgccattatc gttgcggcga tgctgatcgg ctttatcgcc
    20521 ctgatcgccg cgctgaacgc actgtttgcc accgtgactg gctggtttgg ctacagcatc
    20581 tccttccagg gcatcctggg ttacatcttc tatccgattg catgggtgat gggtgttcct
    20641 tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc caacgaattc
    20701 gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc ggaaggcatc
    20761 atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcgggtgcg
    20821 gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt
    20881 tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac
    20941 catacataaa aaagccgggg ataattcccc ggctttttta cgcctgttaa tcggctaatg
    21001 gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg
    21061 cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca
    21121 gatcggtaat tgatctcaca atcatcgcat ccagcgtgtt cgtgacgata tctttcacaa
    21181 agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat
    21241 atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt
    21301 tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg
    21361 cctgcgggga gatgtaataa cgcttaaaca gacgaataat ccgcccggca atattctttt
    21421 gcagcagcgt gagcggcatt aaattgactg aaaatcgtgg accttttttg tcgcaagggt
    21481 gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt
    21541 taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg tcatacttca
    21601 gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga
    21661 gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag
    21721 tggtttgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga tgatgtgcgc
    21781 aggattgctc tgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc
    21841 catcaaaatg cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa
    21901 tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc
    21961 tcagcactaa cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca
    22021 acggcagcaa cgtacggtaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat
    22081 gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt
    22141 gctccggtgc ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tcggtcagcg
    22201 cctgcaaatg ccagcggcgg ttaagccatt cactacgatg ataaatacgc accatataga
    22261 gcagacaaac actaaaggaa atcagtactg cgagaataaa tgccagcgaa tattcggtta
    22321 gtaccccttg caaaaagttc tggttgtaat tcagcaggca aagcgttgaa acagcccagg
    22381 tgagatttaa aaacggatag cgaagcttac cgacaccgag ggtaaagatg ataaagaaaa
    22441 caggtaccaa gtaaccggca ataaagtcat tttcatacgg tgtgcacatc agaagtagca
    22501 gcacgctaag agctgccagc cagcttaagg taaatgcgcg tttctctttg ctcaacgacg
    22561 gagcgatatc cctacgccac aatatctgtg caaagtgggg acttacaatc atgcgggtga
    22621 gatagtaaaa aagcatgttg taaatcagca ctgcggtgaa aaggcttagc aaatcaacga
    22681 ccgtgaaaat ggcatccgca tcgccgaaaa aggtagatat ctttagcgga aaatcaaaga
    22741 aatttcccac aagatacatg ctgcatttga tgccaatcgg cgccaccaaa ccaagccaga
    22801 acagacgttg ccagacgtat cggctggtca atccataacg ccaacgcgtc cccaattgcc
    22861 agcgtaatat tgcacaggca cacaggactg caaacgcctg gctgccgatc atgacggcgg
    22921 tctgtaaaaa agtgagatga aggttccaca gattggtgca aaacatccca cataaaatgg
    22981 gaacgacacc acgccagcca aagataaaca agattgacag cattacgcac agtggcatcc
    23041 acgccagaaa gatataattg gaatggataa cagcaagtgg cgaaattaaa cgggatagct
    23101 gaataagtac cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt
    23161 tatgctccac aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata
    23221 ataagtttat tcgcatcgcg aagcaaacag gggccagcct gttgctgttt ttgcagtcag
    23281 tgatgcattc accacacggc actgccagga tgtagactgt attacaaaag cggcaaaagg
    23341 cagagacaaa aaacccccgc tttgcagcga gggtttgaaa tttggtggag ctaagcggga
    23401 tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccac
    23461 gagtcgttta cgtaccaaat ttgctgggtg caaaatttgg tggagctaag cgggatcgaa
    23521 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccatcacgt
    23581 aaagcttgtc gagttgacgg gcggcatcat atgaattccg cccgaatgtg tcaacggcaa
    23641 attgccacgt gtagtttcaa tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg
    23701 ctcgcattca gtagttattc atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact
    23761 actaatagac ccacatacat tcagggaatt gttatgttca aggagaggat gacgccagat
    23821 gaacttgcca gactgaccgg ctatagccgc cagaccatta ataaatgggt acgcaaggaa
    23881 ggctggacaa cgtcaccaaa accaggcgtc cagggtggca aggctcgact ggttcacgtc
    23941 aatgaacagg ttcgtgaata tattcgcaat gctgaacgtc cagaaggtca gggagaagcg
    24001 ccagcccttt ctggtgatgc gccgcttgaa gtgttgctgg tgacactggc gaaagagatg
    24061 acgccagttg aacaaaaaca gtttacatcc ttgcttctgc gggaagggat tatcggattg
    24121 ttacaacgct tagggattcg cgatagcaaa taatatgaaa agattacgca ataaaatgac
    24181 caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa actgttaacc gttggatcag
    24241 agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat
    24301 tctggtcgat acgcaagttt gcgagtttat tcagaacacg ccagccttcc ataacacacc
    24361 gatgcttctg gaagccgaag aaccccttgc cgaatacgca ccgggtattc gcactcctgc
    24421 ttatcggcaa atcatcagcg caatagacaa catgactcac agtgagcaag aaaaagttgc
    24481 gcaatttttg tcacgcgaag gaattcgtaa ttttcttgct cgtctcgaca tagatgaatc
    24541 agcataaata aaaaacggca ggggtatctc tcctgccgtt tatcttttat gcgctaatta
    24601 ctgctgattt tcgcgttcag caataaaagc cagcgctttg ttgatacgct cgatactgcg
    24661 ggtcttaccg attgcgtgaa cggtaacatc cagcgccgga gactgccccg caccggttac
    24721 ggcgacacgc agcggcatac caactttacc catacccact tccagctcat ccgccgtcgc
    24781 ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta atcgcagtca gtttgtcacg
    24841 aaccacttcc agcggctggc gcgctaccgg acgcagatgt tttttcgcgg cgtcggcatc
    24901 gaactcagca aaatcttcgt agaaataacg gcagctctgt gccatctctt tcagcgtctt
    24961 gcagcgttcg cccaacagtt tcaccagatc agccagctgc gggccgttac gggtatcgat
    25021 attttcctgc tcaatgtgcc actgtaagtg agtagcaaca tactccggcg gcaacgcgtt
    25081 aatgtagtga tggttcagcc acagcagctt gtcggtgttg aacgcactgg cagatttgct
    25141 gacggcattc agagtgaagt atttaatcat ctcttcacga gtgaagattt cctgatcgcc
    25201 gtgggaccag cccagacgca ccagatagtt cagcagtgct tctggcagat aaccatcatc
    25261 acgatactgc attacgctga ccgccccgtg acgtttggac agttttttac cgtcatcgcc
    25321 gttgatcata gaaacgtgcg cataaaccgg taccggcgct ttcagagcct taagaatgtt
    25381 aatctggcgc ggtgtgttgt tgatatggtc ttcgccacgg ataacgtggg tgatttccat
    25441 atcccagtca tcgacaacca cacagaagtt ataggttggg gaaccatcgg tacggcggat
    25501 aatcagatca tccagctcct ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa
    25561 aacaacagaa ccttcctgcg ggttagcaaa acgtacgaca cacggctcat catcagcgtg
    25621 atgctcatga ctgtggcggc agcgaccgtc ataacgcggc ttctcacctt tcgccatttg
    25681 ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag catttataag ctgtgccctc
    25741 ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg
    25801 accttcatcc cactccaggc tcagccagtt catgccatcc ataatggctt cgatagcttc
    25861 cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc acgaactcac cgccgtggtt
    25921 acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt
    25981 tgggcttggc gcgaagcgag ttttgatttt catgaaatgg ccttacgttt agaaagatgc
    26041 cgacaaccgg caaatcctgg aaaaattgag tgggcgatat tctatcactc ccgcctgatt
    26101 cctcaatgta gttcgggttg caaccttacg cattattgtt ttcgttgaca aattgcgcaa
    26161 cacggtctgt tttgcgatca tcttgcttaa atttacgacg aacgaacaat ttctttagaa
    26221 aatgcgttga ctcattttga actctcccta taatgcgact ccacacagcg ggggtgatta
    26281 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
    26341 cccaccaact actttatgta gtctccgccg tgtagcaaga aattgagaag tgggtgatta
    26401 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
    26461 cccaccactt tctcgccagc tagatttctt gtaacaatgt gaagtaccga agtgggtgat
    26521 tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat
    26581 cacccaccac ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
    26641 cgcaggttcg aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgttttttt
    26701 gctatctgca atttgcgaaa ttgcctgatg cgcttcgctt atcagaccaa ctatgtccgg
    26761 cagttcccgt gcctctcatc tactgcgcca acgccgccag cagtttaacc agcgcgggcg
    26821 tcacctgctg cgtttcataa acaatatata agtctgccgg gatgcgttct ttgagcggac
    26881 ggaatatgac accgggccag ttcatctgcg cgtagctgtc cgctatcaat gtgataccaa
    26941 tacccatact gaccatggcg agtaccgttt gcggttcatt aacttcgcga ataacaaccg
    27001 gtgaaaatcc cacctgctgg caaactcgct gcaaaaaatc ccagtcagtg tacacgggcg
    27061 gcattgtaac gaaatactcg tcacgtagcg cttccagcgg gacggtggaa aatgatgaga
    27121 gatgatgctc ttcaggcatc gccaccagaa acgccgattc atgcaaccgt aagctggtaa
    27181 aaccagtcgg tggttctgtc gccattcgcc agatcccggc atcaagttcg cggcgttcca
    27241 gcaaggccat ttgcatcgcg ggcatctttt cgcgaaacag aacttcaacg ttaggatttt
    27301 ctctaaggaa tcgccgcata accgggcgca tccgtcccca cattgccgtt cccacaaccc
    27361 cgagttcaat ccgccctgct tctccccgac ctatttgttc aacccgagcc aatacattat
    27421 ttgcattcac cagcaatcga cgcgattctt ccatcaagat tttgcccgcg tgtgtcagta
    27481 cgacgctgcg cgaatggcga ataaaaagct gcgtgccgag ttgattttcc agctctttaa
    27541 tatgaatact gagcggaggc tgagacatat ttaaacgcgc tgctgcgcga ccaaaatgca
    27601 actcttccgc tacggcaaga aaataacgga gcaacttaag atctgttcta tatacgcgtt
    27661 ccataattaa gtgctccata taataaatac atcgtattaa attatcatgg cataaagtat
    27721 ttcaccacca gatatccgac atacataaac acgatggata atatacccgg caatacattt
    27781 ggaacaggaa taggtaatcg cacgattgta aacaaagcac caatgccaaa accaacgcca
    27841 gaagaaaata aaatatcttt cactaattaa cctttatcat aaaagcagcc ccaaggaaca
    27901 gagctgcgaa tccttttaat gtgtaactga acgatgcgtt atcttttcag ggtcatggtt
    27961 atatttaaac ccaaagaaaa atatcactgc gaggaaaaga gcataccctg caaacatcag
    28021 ccaaatggta tgccaatctt ttacgccatc caccgagaag taatccactg ccataccgct
    28081 cagaatcgag ccaacccatg cgccgacgcc atttaccatc gtcataaaaa ggccctgggc
    28141 gctggcacga atgctggaat caacttcctg ttcgacaaag accgaaccag aaatattgaa
    28201 gaaatcgaat gcacaaccgt aaacaatcat cgacagcaac agcaaaataa acccggttgg
    28261 tgatggatcg ccataggcaa agaagccaaa gcgcagcgtc cacgccacca tactcatcag
    28321 catgacggtt ttaataccga aacgcttcaa aaagaacggt atcgtcagaa tgaaaccgac
    28381 ttcagccatc tgtgaaactg acagtaaaat ggacgggtat ttcaccacga agctatcagc
    28441 aaactcagga ttacgggcga aatcatgcag gaacgggttg ccaaaaacgt tggtaatttg
    28501 cagcaccgca cccagcatca tggcgaagag gaaaaagata gccatgcgtg gatttttaaa
    28561 cagcacgaag gcatccagac ccagcttgct ggcaagcgat gtagtcgctt ttttctccgc
    28621 aaccggaatc ttcggcaaag tcagcgcata agccgacagc agcaatgacg caccggacgc
    28681 gatatacagt tgcagactac tcaattccag atgcagcaag cttactgccc acatggcgac
    28741 aatgaacccc accgtaccaa aaacgcgaat gggcgggaaa gcggtcaccg ggtcaagccc
    28801 tgcctgggca agacaggaat aagagacgct gttcgataac gcattagtcg gcataaacgc
    28861 catcgcattg actaacatca cccaaaacat catatccgga tcagttacgg atgccgcata
    28921 aaaaagtacg cccgcacaca ccaggtgaca cagcatgtat gcacgttctg cacgcagcca
    28981 tttgtctgcg atgatcccca ttataccagg cataataatc gcggcgatcc ctttggaact
    29041 gtaaaccatg ccaacattag cgccggtgaa atgaagggta ttaatcatgt aagaaccgag
    29101 ggtaaccagc cagctccccc agataaaata ttgcaaaaag gacattacct ttaagcgcaa
    29161 agcgatgctc ataatcgttt ccttgtcgca tcatatggcc tcactcagta aggccgttta
    29221 ttgttgtttt actcaggcaa ttttgcgcag aaagccgcaa ataagattaa taaagttttg
    29281 ctttgagagt tccgctgctg ccagcgtttg ggcatgagaa agcttcacgt cactcagacc
    29341 ttccgccatg ttggtaatcg cagagaccgc aacgacttta agttcgcaat ggcgagctga
    29401 aataacctca ggcaccacag acataccaac aacgtcccca ccaataattt gcatcatgcg
    29461 aatttccgcc gcagtctcga aattcggccc cggatacgag acgaacacgc cctccgtcag
    29521 agggaccccc tcttctttcg ccactttttg taacagtgcg cggtattccg catcgtaggc
    29581 attcgccagc gagaagaagc gctcaccaaa acgttcatca ttaagaccca ccatcggcgt
    29641 tcccggcatg gtgttgatgt gatctttcaa tgcgaccaga ctgccggccc ccacttcagg
    29701 gcgcagtgag ccagccgcat tggtgcagaa cagcaactcg cagcccagca acttaaaggt
    29761 acggattgca tccgtcatga tggtcatccc acgaccttcg tagaaatgtc cgcgaccttt
    29821 catgcacacc actggcaccc cctggagata ccccagcacc agctcacccg catgaccgtg
    29881 tacggtactt accgggaacc caggcagctt ttcgtaggaa attgcgaccg cgttctcaat
    29941 ctgatcggcc agcgcgccca gcccggaacc taaaataaag gccactcgtg gcgtgaaatc
    30001 aggtttataa gtcttgataa tatctacgca atacagtggg ttatgagaaa actgagcctg
    30061 atacatatat atcctttttt tgtagggggg aatctaacgc ctttttataa cgagattgcc
    30121 ctacctcata ccaatacact tttttcatgt atccgatagt tattcgatat cgatatattt
    30181 atattttttc actattaata ggctgaaaaa actgtattgg tactctcctt tcccttgcat
    30241 taacaatatg ccgcttttaa tcaacctttt tattaagcaa tcaactaata acgatcggca
    30301 tagggaatta tgctatgaaa aaacatcttt ttactctgac actctcttct gtattggcga
    30361 taccagcagt atcccatgca gaatttaaag gcggttttgc tgatataggt attcattatc
    30421 ttgactggac tagcagaacc actgagaaat cgtcgaccaa atcacacaaa gatgattttg
    30481 gatatctgga actggaaggc ggcgctaact ttagttgggg agaaatgtac gggttcttcg
    30541 actgggaaaa cttttataat gatcgtcatg ataaaccggg tagcgagcag cgttatacat
    30601 ttaaaaatac caaccgtatt tacctgggtg acaccggatt taatctctac ttgcatgcgt
    30661 atggcaccta cggttctgcg aatcgtgtaa atttccatga cgatatgttc ctttacggga
    30721 tcggctacaa tttcaccggc agcggttggt ggttcaaacc gttctttgca aaacgctata
    30781 cagaccaaac ctattatacc ggcgataacg gttatgtcgc cggttgggtt gcaggttaca
    30841 gctttatgct gggcagtgag aaattcaccc tcaccaactg gaacgagtac gagtttgatc
    30901 gtgatgccac ctacgcggca ggtaatggcg gcaaagaagg tctgaacggt gccgtcgcac
    30961 tttggtggaa tgcaacttca cacatcacca cagggattca gtatcgttat gcggatgaca
    31021 aactgggcga agatttttac caggatgcga taatctactc catcaaattt aatttctgat
    31081 tgattactgc cggagctccc ggcagtatga attcactcta tatctgatac aacggatccc
    31141 cttcccgccc agccattagc gcattgccct ggtctcgtac tacgtcccat aacgcttcag
    31201 cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt tcaaccactg
    31261 gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc agtgcgggaa
    31321 gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga tgtccaatct
    31381 cctgcacaat attcgcctga ataccgttgc gcgccagcgc tgcgtcaatc agcggtcggc
    31441 tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc caggggacat
    31501 aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc tcggaaagaa
    31561 tcgcttcaca ttgcagatcg cccactgggc caggatcgat gacgatgcca aaatcgacat
    31621 ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg acaaactgaa
    31681 tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc gcggaaatgg
    31741 tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc ccggtatcgc
    31801 gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac tgctgccctg
    31861 catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca gtatgatttt
    31921 ccagctcctt cacactgtga ctcactgccg actggctcag gccgatacgc tctcctgcac
    31981 gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgcttt aaagaataat
    32041 tcatctgttt ttttcatgaa tggatgcaat aaatcaattt tatttctcaa actggaagac
    32101 gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat cctcgatcct
    32161 ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc cagaggcgat
    32221 ttcgtcccct tctttgaaaa tctgaccacc gcagcgatag ccctgctgtt ttttatgcac
    32281 ggcgcgaagt tatcgcgtga ggcgattatt gctggcggtg gccactggcg actgcatttg
    32341 tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gcgtgctgtt tgcctggtgg
    32401 aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg cattctcccc
    32461 gcgaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt cgcggcggcg
    32521 gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt gctggttggt
    32581 ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa aattatgctg
    32641 caactgttgc tgccatttgt gttggggcat ctttcccggc cgtggattgg tgactgggtg
    32701 tcgcgtaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt ggtagtttat
    32761 acggcattca gcgaagccgt cgttaacggt atctggcaca aagttggctg gggatcattg
    32821 ctgtttatcg tggtggtcag ttgcgttctt ctggctatcg tgattgtagt taacgtcttt
    32881 atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt ttgtggttcg
    32941 aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac atcggtgatc
    33001 ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt ctgtgcggtg
    33061 ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga aagcagcgcc
    33121 gataaagctt aaagcggacg tttcaggggc tggaccagtt gcgtcagccc ctcgatttta
    33181 atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc atctttgcgg
    33241 gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt gtacttgcca
    33301 aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc agctacccag
    33361 caaacgcagc atttccgttt cgtcgatgac ttcaataccc agttcctgcg ccttcgccag
    33421 tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca cgctgcccgc
    33481 gactttcgcc cccagttcga ccagtcgagc tttagcgtca tcacgcgaca tctggcttaa
    33541 gctgcccgta agcaccacgg ttttaccagc aaacggactg tcaatctctt ccgcattgat
    33601 aacgatcggc tcaggccagt gaacaccttc cgccaacagc tcgctgatga cattgcggtt
    33661 gctttcttcg gcaaagaagt tgtgaacgtg ggaggcaacg acaattccaa catcaggcac
    33721 cttttgcagc tcttcaatcg acgcggcttc cagcgcttcc agcgtgccga aatatgccgc
    33781 cagacctgct gcggtagcct cgccgacttc acggatgcca agtgcataga ggaagcgggc
    33841 aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg atttcggccc
    33901 catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatccg ccggagtgtg
    33961 gacatattct ttttcaacca gttgatcgat gattttgtcg cccattccgt caacatccat
    34021 cgcacggcgg gaaacaaagt gtttcagaga ctctttacgc tgcgcaccgc aaatcaggcc
    34081 accggtacag cgggcaaccg cttcaccttc cacacgctcg acgtcagaac cacataccgg
    34141 acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa gcacgacgtt
    34201 aaccacctgc gggatcacgt cgccagcgcg gcgaataacc actttatcgc caatgcgtaa
    34261 accaagacgt tcgatttcgt cagcattatg cagtgtagcg ttactcacca acacgccagc
    34321 aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc caacctgaaa
    34381 ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg ctaccgccca
    34441 gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt tgaccttaat
    34501 caccacgccg tcgatatcaa agcccagcgt cggacggtct ttttctactt tgtgataaaa
    34561 cgccagcact tcttccgccg attcacaaag tgttacccga tcactgacag gtaatcccca
    34621 tgctttaaat tgcattaagc gtcccaaatg ggtatccggc aattcaccgc cttccagaac
    34681 accaacgccg tagcagaaga aagtgagcgg tcgcttcgct gtaatacgcg gatcaagctg
    34741 acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc ccgtgcgtcg
    34801 cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac cacgcacttc
    34861 cagacgcgcc gggatattct caccgtgtag cttcagcgga atggcgcgaa tagtacgcac
    34921 attagacgtg atatcttccc cggtagtgcc atcgccacga gtcgcggcac tgactaaaac
    34981 gccattttca tacagaatac tgacggcaag accatccagc ttcagctcac agcaccaggt
    35041 gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaagcaa gaaagctttc
    35101 ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcggatct ggctgaaagc
    35161 cgccagcggc gctgcaccta cacgttgggt aggcgaatca ggcgtaatca gttctggatg
    35221 tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt cgggaatttc
    35281 cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc gcagttctgt
    35341 cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc cgacaagcgg
    35401 gggttcgaaa aggagttaat ttgccttaag tgtatcaggc gttggcgtct ttgacttcgc
    35461 ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc tgatcgtcaa
    35521 gcactacacc acccacttca tcggcaatat gctgcgcaga ttgcagcatc agcttgaagt
    35581 tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc ggagtagtga
    35641 aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg ctgaataacg
    35701 ccgggccgct gccatccggg ctaagatgac gatggtaaat gttcatatca ccaaaaatga
    35761 agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt taactcgcta ccgtgatgcg
    35821 ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata actggagccg
    35881 gttccgcaac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg aaagcctgtt
    35941 gtgccggttg cggtgctgaa tgcactggct gcggcgctga cgcgacctgt ggcgaaactg
    36001 gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg ggttgctgca
    36061 ccggctgcgt tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc ggctgctgga
    36121 ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc ggcgacggac
    36181 gagcaacctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga acacgaacct
    36241 caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca cgttttgact
    36301 tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg ctggtccaga
    36361 aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat atcagacgca
    36421 aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc aaacatttac
    36481 tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt tcacagcaca
    36541 aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact gtccattgcg
    36601 caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag ctcatcctga
    36661 ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt gcgcaaggct
    36721 ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg ctggtcaata
    36781 ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc tggatcccga
    36841 ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg tggcccctgg
    36901 ctgtcatctc tgtgctgtta gtgtttggct atttcttctc aacgattgct aactggattg
    36961 ctgctccgtt caacggttta ttggctgaac aactggaagc aagactgact ggcgctacac
    37021 cgccagatac cggaattttc ggtatcatga aagatgtgcc gcgaatcatg aaacgcgaat
    37081 ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt gctaattctt tacttcatcc
    37141 ctggcattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc tggatgttag
    37201 ccatccagta ttgtgattac cccttcgata accacaaagt gccgtttaaa gagatgcgca
    37261 ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc agcctgttta
    37321 cgatgatccc gctgcttaat ctgttcatta tgcccgttgc cgtttgtggc gcgacggcga
    37381 tgtgggttga ttgctatcgc gataaacacg ccatgtggcg gtaacaatct accggttatt
    37441 ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc atcttgcatg
    37501 tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata ttctctgagc
    37561 gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat gagtaagatt
    37621 tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa tcgcatcggt
    37681 aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt taagtgccgt
    37741 atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc aggcgttgaa
    37801 ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtagc tgccgctcgc
    37861 ggttacaaac ttaccctgac catgccagaa accatgagta ttgaacgccg caagctgctg
    37921 aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa aggcgcaatc
    37981 caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct gcaacaattc
    38041 agcaatccgg caaaccctga aattcatgaa aaaaccaccg gtccggagat ctgggaagat
    38101 accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac gctgactggc
    38161 gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt cgccgttgag
    38221 ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa acctggtccg
    38281 cataaaattc agggtattgg cgctggtttt atcccggcta accttgatct caagctggtc
    38341 gacaaagtca ttggcatcac caatgaagaa gccatttcta ccgcacgtcg tctgatggaa
    38401 gaagaaggca ttcttgctgg tatctcttct ggagcagctg ttgctgcggc gttgaaacta
    38461 caagaagatg aaagctttac caataagaat attgtggtta ttctgccgtc atcgggtgag
    38521 cgttatttaa gcactgcatt gtttgccgat ctcttcactg agaaagaatt gcaacagtaa
    38581 tgccagcttg taaaaaatgc gtaaaaaagc acctttttag gtgctttttt gtggcctgct
    38641 tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt taaccagact
    38701 aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa tcgattttat
    38761 gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa acgtcagcgg
    38821 tcaacacccg ccagcaatgg actgtattac gctcttcgtg cgtcgcgtct gttaaaaact
    38881 ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc cacaacacta
    38941 aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc gctccgaacg
    39001 gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc acttctgaaa
    39061 ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa ctgcagactc
    39121 tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac gagcagaaag
    39181 cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg ttcttttaaa
    39241 aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc cgggtatcgc
    39301 tttcggtaaa gctctgcttc tgaaagaaga cgaaattatc attgaccgga aaaaaatttc
    39361 tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca aggcatcagc
    39421 ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa aagaagccat
    39481 ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa tcatagccct
    39541 gattaaagat aagcacatga cagctgacgc agctgctcat gaagttatcg aaggtcaggc
    39601 ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg acgtacgtga
    39661 tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc tgagcgccat
    39721 tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg cacagctgaa
    39781 cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc acacctctat
    39841 catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca cctctcaggt
    39901 gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg tcaatccaac
    39961 caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg aaaaagcaga
    40021 gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg tagaagtgtg
    40081 cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg ctgaaggcgt
    40141 tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgttgccca ctgaagaaga
    40201 acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg ttatcgttcg
    40261 taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga aagaagagaa
    40321 cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga tcctgcgcga
    40381 tcagctccgt gctatcctgc gtgcctctgc tttcggtaaa ttgcgcatca tgttcccgat
    40441 gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct acaaacagga
    40501 actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa tggtggaaac
    40561 accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct ttagtatcgg
    40621 caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata tgatttcaca
    40681 cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta ttgatgcttc
    40741 tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg aacgtgctac
    40801 acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt ctatcccgcg
    40861 cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag cagagcaggc
    40921 tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca ttgaagaaaa
    40981 aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag atcatgggtt
    41041 tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga actattgaga
    41101 tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat gtcgtttttg
    41161 cggaaaaaat cgttggtgat ggcattgcta tcaaaccaac gggtaacaaa atggtcgcgc
    41221 ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcgttctct atcgaatctg
    41281 atagcggcgt tgagctgttc gtccacttcg gtatcgacac cgttgaactg aaaggcgaag
    41341 gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggtgatact gtcattgaat
    41401 ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt gttatctcca
    41461 acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgta ggtgaaaccc
    41521 cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc atcggcgcca
    41581 tttttttatg cttcagccag aggcggcaaa atcaattcat cgctctcatg ctgctgggta
    41641 tagcgcatca cttccagtac gcgcaacccc gcgcggtgca ccgcatcggt taacgccttc
    41701 cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc tttcaggtca
    41761 gttttaaccc gggaatggga aatgacattc acgctgtcgg cactgaccac cacaacctgc
    41821 atctcctgat tttcttcatt gccggaggcg ctggtaatca ccacccactt taatgtgtct
    41881 gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatc tttaccggtc
    41941 aggatttcca gctcaaagat attgggggta attccctgtg ccagcggcag taaatattgt
    42001 cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc gccaatcacc
    42061 ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag ccactcggca
    42121 aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg aagttggcgc
    42181 agcgcatcac gctcctgaag cgcacgcaaa taaccgctaa accattcatc cggaatcgca
    42241 ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg cacggcaaag
    42301 acattcaggc cgttctgctt tatagcaggc accgcaatgc tgttgcctac gctgccgtac
    42361 actacctgcg actgcacggc gacgatatcc gcctgcagtg ccctgctctt atcgttaaac
    42421 aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg tattccagcc
    42481 gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaggat aaggtaattc
    42541 agtgaagaaa atcatttgtc tggtcgttac gctgctaatg acactccccg cttatgcgaa
    42601 gttaacggcc catgaagaag cccgtatcaa cgccatgctg gagggattag cacagaaaaa
    42661 ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg tttctcatct
    42721 gcgcctgaag ctgggcaata cccgtaatcg cattgataca gccgagcagt ttattgacaa
    42781 ggtggcctcg tcgtcatcga ttaccgggaa gccgtatatc gtgaagatgc ccggtaagag
    42841 cgatgagaac gcacagcctt ttttacatgc gttaattgcg cagacggata aaacggtgcc
    42901 tgcgcaataa tcttaaatcc ccgccccctg gctaaaatgc tcttccccaa acaccccggt
    42961 agaaaggtag cgatcgccac gatcgcagat gatcgccacc accaccgcgc cagggttagc
    43021 ttttgccacc cgcagtgctc cggcaaccgc gccgccggag cttacgccgc agaaaatccc
    43081 ttcccgcact gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa tatccagcac
    43141 ctcatccacc agagaagcat tgaaaatccc cggcagatat tccgcaggcc agcggcgaat
    43201 gccgggaata ctgctgcctt cttccggctg caggccaaca atggtcaccg gtttggattg
    43261 ttcgcgcata aagcgtgaca cgccggtgat agtgccggtt gttcccatgc tggagacaaa
    43321 atgggtaata cgcccgccag tttgctgcca gatttccggc ccggtagtgg tgtaatgggc
    43381 gtaagggtta tcgggattat tgaactgatc gagcaacttt ccttcgccgc gattcgccat
    43441 ctccagcgcc agatcgcgtg cgccttccat gccctgttct ttggtgacca gaatcagttc
    43501 tgcaccgtag gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa
    43561 tttcatgcga tagcctttaa gcgccgcaat cattgccagc gcaatgccgg tgttaccact
    43621 ggtggcttcg atcagcacat cacccggttt aatctcgccg cgcttttccg cctcgacaat
    43681 catcgaaagc gccgcacggt cttttaccga tcccgccggg ttattgcctt ccagttttaa
    43741 ccacacttca ctgccgttat ccggcgccat tcgctgcaac ttcaccagag gcgtattgcc
    43801 tattgtttgt tctaatgtac tcacgatctc tgtccatacg tggtgttgcc tgatgcgacg
    43861 cttacgcgtc ttatcagacc tacaggttac aaaaccttgc cattaaaaaa cccggatcgc
    43921 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    43981 agttcctcat cgcgggtttc aatacgctca tcgccgttat acagccgcgc atgttgcaga
    44041 ccaacgtata aacgatcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    44101 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    44161 gcttccagta cctgtacggg tagcggcgaa tcgaggctgg tacggcggct gatatccact
    44221 tcccacgggc gcaggaagag atccaccggc ccctgatacg cgggggtgta tcccagcggc
    44281 cagcgatgtg cgccaacgtg gaactgcccg ccgcgaatgg ttccctgcag gcggtttacc
    44341 tcgcccataa actccagcac aaaacgagtc gccggttcac gccatacctg atccggtgcg
    44401 tcagcttgtt caatattgcc ctgactcatc accactacac gatcagctac ttcggtcgcc
    44461 tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gttcttcatg cagttgacgc
    44521 agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc
    44581 agcaaaattt gcggttccac agccagcgcg cgtgcaagcg ccacacgctg tttctgaccg
    44641 ccggaaagct gcgccggata acgatccgcc agatgagcaa gctgcaccat ctccagcaat
    44701 tttgtcactt tcgctttgat cgccgccgca ttcgggcgtt cacgccgtgg caacaccgtc
    44761 aggccaaaag cgatattgtc aaacaccgtc atatggcgaa acagcgcgta atgctggaac
    44821 acgaaaccga ctttacggtc ccgcgcgtgc agacggctca cgtcggtacc gtgaaagcga
    44881 atatgcccac tggtttgatg ctccagcccg gcgataatgc gcagcagcgt agtttttccg
    44941 gaaccggacg gcccaagcaa cgcgaccatc tgaccagaag gaatatccag tgagatatcg
    45001 ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat gctcatgatg
    45061 ttcctcctgc tgcgcgcgtt tttcctgatt ttccaggcgc cactgcaaca tactctttaa
    45121 aaacagggtg ataatcgcca tcagcgtcaa caacgcggcg gcggtaaagg agccgacggt
    45181 gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt cgccacgaat
    45241 cgagccggaa accaccgata ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    45301 cacgccgtaa agcagcgccc agcggatgtt cggcagcgtg acgcgacgga acatctgcca
    45361 gccggacgcg ccaagtaaaa tcgccgcttc gtcttcctgg ctgccctgac ttaacattac
    45421 cggtaccagt tcgcgcacca caaacggaca cgtcacgaag atggtgacca gcaccatacc
    45481 cggccaggag aacataattt gcagattatg ttcatccagc cagccgccca gcgggccgtt
    45541 agagccgtag aacagcagat acaccagacc agcaaccacc ggcgacacgg caaacggaat
    45601 gtccagcagc gtcagcaata gctggcgtcc ggggaagtta aagcgcgtca ccagccaggc
    45661 cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    45721 ccagatggcg tgcagcatgt ccggatcggc cagattttgt aacaccggca tcagtccttt
    45781 gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgataccag
    45841 catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    45901 caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    45961 gtgttaattg agaacagcaa cagcagcgat gccgcgagga tcaccgaggc aatcgcgctc
    46021 gccgccgggt aatcaaactc ctgtaagcga acaaagatca tcagcgacgt cacttctgtt
    46081 ttccaggcga tattcccggc gataaaaatc accgcgccaa actcaccgag gctgcgggta
    46141 aacgacagcg ccacacccgc caccagtgcc ggagatagtt ccggcagcac cactttgcga
    46201 aaactctgcc agcgtgttgc gccgagcgtt tctgccgcct cttcatattc cgggcctaac
    46261 tcttccagca ccggctgcac ggtgcgcacc acaaacggaa tgctggtaaa ggccatcgcc
    46321 accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag ccattcaccg
    46381 taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    46441 gcaaagggta aatccatcag cgcatcaagc agcgtgcgac ccggaaagcg atagcgggtt
    46501 aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac
    46561 agcagcgtca ctttataggc tgcgaccacc tgcgggttgg tgatcacctc ccagtactgc
    46621 gcccagctca tctgggccag ttgcatcacc agcgcggaga gcggcagcag caaaatcagg
    46681 cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac gcgtcttgaa
    46741 gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    46801 tgaagtgggt tttcatcact tccggccagg agccaaattt atcttccacg cggaacagct
    46861 cggtctgcgg gaatttatct ttcagtttgc ccatgacttc cgggttattt acgcggtagt
    46921 aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg taggcttttg
    46981 ccgctttttc cgtgccgttg gcctgtacgt ttttatccac ccacgccacc gggaattccg
    47041 ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac tgtttacgga
    47101 tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc tcggcaaaag
    47161 tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg aactgggtca
    47221 taaactgttc ggttttggct ttatcgccac cgtcggcttt atccgctgca ccccacgcgg
    47281 ctaaataggt gtaacgcgcg ttaccggagg ttttcgggtt cggaaaaatc agcttcacgt
    47341 cggagcgcac cagatcgttc cagtcgtgga tattcttcgg gttaccctta cgcaccagga
    47401 agcccatggt ggagtagaac ggcgagctgt tgtttggcag acgcgactgc cagtcagccg
    47461 gaataagctt gcctttgtcg tgcaggatct gcacgtctgt cacctggtta taagtgacaa
    47521 cgtcggcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt
    47581 gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca aacggcgggt
    47641 tcagggcggc aaacagctcg cgcgagacgt cataagaact gttcagcagt tccgttgcct
    47701 gtacatggcc cgccagcagc agagaagcga ccagcacgag tgagttcttt ttcagtaagt
    47761 taacggccat tgcgcaccct tataaattta atgactttct aatagccatc atatttataa
    47821 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga
    47881 ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca gatgccgacg
    47941 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc tgttaaatag
    48001 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    48061 cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc ttccgggttc
    48121 gactggcggg caatgctttc cgccattggc gtgcgaacgt atccagggca gatggcgtta
    48181 acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    48241 gctttcgtta aggcgtacgc cgtttcgccc gggtctgcca ccatatcgcc agtgactgaa
    48301 gacatcatca caatgcggcc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg
    48361 acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc atcgctcata
    48421 tcgaggaagc tgcccagacg acaaacgcct gcgttattca ccagaatatc aatgcgtcct
    48481 tctttttcct tagcgcgctt aatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    48541 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca
    48601 ggggagatat ccagcaagat taggtttgcg ccatgacgtg caaaagttct ggcaattcct
    48661 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    48721 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    48781 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaattt agtcgctgct
    48841 ttatgcagca cctcaagcgt tacgtcagcg cgcaatcatt ttcagtgaca tcacatgagt
    48901 aatattcgga cttaatgatt cctttgtgtc tatcaaatcg ggatatatga tcccatttat
    48961 ctgcaattat ttgatcgcaa atattaaggt caccaccgat gcaacttgaa aagatgatta
    49021 ccgaaggctc gaacgccgcc tcggctgaaa ttgaccgcgt atcaacgctg gaaatgtgcc
    49081 ggattatcaa cgatgaagat aaaaccgtac cactcgccgt tgagcgcgta ctgccggata
    49141 tcgccgcggc gatcgatgtt atccacaccc aggtaagcgg cggcggccgt ctgatttatc
    49201 tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt ccgccgacct
    49261 acggcgtgaa accgggcctg gtagttggtt tgattgctgg cggcgaatat gccattcagc
    49321 acgcggtgga aggcgcagaa gatagccggg aaggtggtgt taatgatctg aaaaatatcg
    49381 gcttaaccgc gcaggatgtg gtggttggta ttgcggccag cggacgcacg ccgtatgtga
    49441 ttgccgggct ggaatacgcg cgccaactcg gctgccgtac agtgggcatt tcctgtaatc
    49501 cggggagtgc cgtttcatcc accgcagagt ttgctattac gccggtagtt ggtgccgaag
    49561 ttgttaccgg ttcttcgcga atgaaagcag gcacggcgca gaaactggtg ctcaacatgc
    49621 tctctaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg gtcgatgtgg
    49681 tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgtcaaaaac gctaccggat
    49741 gtaatgccga acaagcggaa gcggcgttaa ttgcttgcga gcgcaactgt aaaacggcca
    49801 tagtgatggt actgaaaaat ctcgatgccg ctgaagctaa aaaatgcctg gatcaacacg
    49861 gcggttttat tcgcaaggct ctggaaaagg aataacccat ggctaaagag atcagcagtg
    49921 aacttctaaa caccattctt acccgggtcg gcggaccggg aaatatcgcc agttgtggta
    49981 actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat cccaatatca
    50041 aaacgctgga aggcgtgaag ggggtcattt tgaccagcga tcaggtgcag gtggtttttg
    50101 ggccaggcaa agcgcatcgt gccgcaaaag ccatgagcga gctgctgggc gaggcaccgg
    50161 tacaggatgc cgcagaaatc gccgcccaga ataaacgcca gttaaaagcc agacaaactt
    50221 ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg atccccggtt
    50281 ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg atgcacgttc
    50341 cggcagatgc tcagggcacg ctacccgatg cgctgaattt tatgaaggtg ttcagcaaag
    50401 gcctgttcac cttcctggta attctggtgg gctataacgc ggcccaggca tttggtggca
    50461 cgggcgtaaa tggcgcaatt atcgctgcgc tctttttgct cggttataac cccgctgcaa
    50521 ccacgggtta ctacgccgga tttcacgatt tctttggtct gcctatcgat ccgcgcggca
    50581 atattatcgg cgtgttgatt gccgcatggg cctgcgcccg catcgaatgc atggtgcgcc
    50641 gctttatgcc ggacgacctc gacatgctgt tgacctcatt aatcaccctg ctgatcaccg
    50701 ctacgcttgc gtacctgatc attatgccgc tgggcggctg gctgtttgaa ggtatgtcat
    50761 ggctgtttat gcacctgaac agtaatccgc tcggttgtgc ggttttagcc gggctgttcc
    50821 tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttatctg gcgttaatgg
    50881 acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgca ggccaggtgg
    50941 gcgcggcgct ggcgctctac tggcgggcgc aaccgcacag tgcattacgc agtcaggtcc
    51001 gcggggcgat tattcccggt ctgcttggcg ttggcgaacc gctgatttac ggtgtcaccc
    51061 tgccccgcat gaaaccgttt gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta
    51121 tcggcctgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttc gggccgtctg
    51181 gtctggtggc gctgccgctg atgacttccg cacaaggcat ccttccggca atggcggttt
    51241 acgctggcgg tattctggtg gcatgggtta gtgggtttat tttcaccaca ctctttggct
    51301 gccgtaacgt caatctggac tgattatgaa acggacaatg ctctatcttt ctctgctggc
    51361 tattagctgt agcgtcagcg ccgcaaaata ccctgttttg gcagaaagta gttgatcaag
    51421 tgtatgcggc gtcaaagcag taataaaaag gccagtcagt tgtgttactg gctggccttt
    51481 aatcaatgaa tcagaaacgc ttacagcgcc atcagcttgt ccagcgacgg tgcgaagtaa
    51541 tagccgccag tgaccggttt ggtgaaacgc aacatcgcat cacgcttacc atcggtatcg
    51601 ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta ggcgcagaag
    51661 tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctgacgaac aatcttcagc
    51721 cctttgccat cttctttcag atcaacgcgg gtcaggtgag aggtttccgg acgttcgtcg
    51781 ccgtcaatct cttcgttggc ctctttggtg cgcccaatca tcatctcttg atcgtgaacg
    51841 ctcatccgat tgagctgctt caggttgtgc tcccaacgct gcacaaacac atagctgccg
    51901 cccgcatcca cgccgtcttt gataacagcc acttcgcggc gcgtctcttc acccgccggg
    51961 ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt caacccaacg gaagccatgg
    52021 atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg agcgacagag
    52081 aagttcacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg ggtcgtcggt
    52141 gcaagacctt taccgtagcc cggaaaatct ttcagctctt cggccccaac gccgccgctc
    52201 agagcgcgcc aggtattgtt accaaaggca accaccgcac caagatgcgc gtccgggaat
    52261 ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg cagggcgtca
    52321 acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc tggcaaaatg
    52381 ccactctgaa cctgagacat tgttcctcct gaaaataata atgccacgca atccgtggca
    52441 ttgttgggcg ctattttacg tgttttttcg cggcggcggt ttgttcagac gcaaattaac
    52501 gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa ggcattaacc
    52561 cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg ccttcggctt
    52621 tacattcgac ggcacgatta tcatcactgt cggccttttg gcaattgcca aatgctttgc
    52681 tgtaaaggtc actaaacggt gtaccgattt taacaccggt gtcagcagga atatcgctat
    52741 ccagcacatc aatgcggctg atcgtgcctt gatcgccgtt aatcaccatc gccacgttgt
    52801 tgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc attccgctgc
    52861 gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt ggtgtggacg
    52921 ccgttaattc acccacgccc tgctcgctca ctttggtgga tgatccaaac cagttccacg
    52981 ggttagcggc cgaccagtta actgacgaca tcgtggaaca gccggtcagc atcaacggca
    53041 tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt caataacgtt
    53101 gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta aaacaagcgc
    53161 gtaaacgtcg attggtcagc agccagatta acgccacgat atccgccact accagcgcca
    53221 gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccaa caaagtaaaa
    53281 ccacttgcgc caacagcaac agaaaataga gcacatgcca gatacgggga aatgaagccc
    53341 gccgaccact cagcaaaaat gccagcaccg ccggaatacc gggaatcaac cccagccaga
    53401 aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg cgcgacgctc
    53461 cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc cagaagagca
    53521 agggtaaacg caggcgaccg tgcgcatcat aatggacagg atgaaactca gtactcttca
    53581 tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc caggcgttca
    53641 tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt cggacagccg
    53701 cgagcaatca gctttttctc cagccgatta agcaacgcat tagcaatccc acgcccacga
    53761 aactcgggat gcacgccaag ataataagca gacccgcgat gcccgtcata accgcccatc
    53821 accgttccga ccacttcacc gttcacctca gcgaccagaa acaaactgac gtcatggttc
    53881 atcttacgct cgatgtccat ttccggatcg ttccacggac tcagcaagtc gcaacgctcc
    53941 caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc catggtattc
    54001 gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt agccaatatc
    54061 tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg aaatgaaaag
    54121 taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata taacatctgg
    54181 aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa acactcactt
    54241 cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga atgtcgcaag
    54301 ccatcgccaa agaagaaccg ttaaaaacca gtaacggaca cagcaagccg aaagcaaaaa
    54361 aaagcggcgg cagacgtgtc gttgttctcg atccaggcca cggcgggatt gataccggag
    54421 cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcaattgcc aagaatgtcc
    54481 gttccatatt gcgtaatcat ggcattgatg cgcgtttaac gcgttctggc gatacgttta
    54541 tcccactgta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg tttatgtcaa
    54601 ttcatgccga cggctttacc aacccgaaag ctgccggtgc ttcggtattt gccctctcta
    54661 accgcggggc aagtagcgca atggcaaaat acctgtctga acgcgaaaac cgcgccgatg
    54721 aagttgccgg taaaaaagcg actgacaagg atcacctatt gcagcaagtg ctgtttgatc
    54781 tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt ctgaaaaaga
    54841 ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt gtggtgttga
    54901 aatcgccgtc ggttccgtcg gtgctggtgg aaacctcgtt tatcaccaac ccggaagaag
    54961 agcggctgtt aggcacggcg gcgtttcgcc agaaaatcgc cacagcgatt gccgaaggcg
    55021 tgatcagtta tttccactgg tttgacaacc agaaagcaca ctcgagaaag cggtaagtga
    55081 tgaaacccga cgcacacctg gttaaacagt tcttgctcaa ccttcaggat acaatttgtc
    55141 aacaactgac cgccgtcgat ggcgcagagt ttgtcgaaga tagctggcaa cgtgaagctg
    55201 gcggcggtgg gcgtagtcgg gtgttgcgta atggtggtgt tttcgaacaa gcgggcgtca
    55261 acttttcaca tgttcacggt gaggcgatgc ctgcttccgc caccgctcat cgtccggaac
    55321 ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg cataatccgt
    55381 atgttcccac cagccacgcg aatgtgcgct tttttattgc cgaaaaaccg ggtgcagagc
    55441 ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt gaagaagacg
    55501 ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggcgaa gacgtttatc
    55561 cgcgttacaa aaaatggtgc gacgaatact tctacctcaa acatcgcaac gaacagcgcg
    55621 gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac tgttttgcct
    55681 ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta gagcgacgga
    55741 aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc ggtcgttatg
    55801 tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact ggcgggcgca
    55861 ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat tatcagccaa
    55921 aagacggcag cccggaagcg gctttaagtg agtttattaa ggtcagagat tgggtgtaac
    55981 tccctcaccc ccactctcgc atccgctgat gcagcgtcag tgacggcttc tcggcaaaca
    56041 gttgctgata atccgtggca aattgcccaa gatgccagaa tccccactgc atggcggcgt
    56101 cttttaccgt tgtgctttgt gaccacggac ttatgagttc gcggcgcacg gcgttcaggc
    56161 gaatacgttt cagccacgcg ttcggaccaa tgcccaaaat ggcgtgaaat gcgttttgta
    56221 gcgtgcggcg gctgacatgc agttgattac acaagtccag caccgtcacc ggttcggaca
    56281 tgttttccag cacatattca cgggcgcggg aaagcaatcg gcggtaactc tgatggctga
    56341 tgctttccgc cgtcatcatc ggctgcgctt cttccagcat cgcccccatc gccatgagca
    56401 aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg ttctcgcaaa
    56461 acgtcgccag cgcctgttgg acaaagcccc acagagcggc tttatgctgc tctttcactt
    56521 ccagcgccga ctggctacgc agcatatgta atacccgatc cggattatgc aaaaagttag
    56581 cctgacgagt gatgacatct tcagaaagca ctacgccaag gatcgtgtaa tcgtccggcg
    56641 tacttaattc aaactcggtt ccacccgggc gcgtcgcaat ttctgcgctt cccagacatt
    56701 gcgaaccgat aaatcccagc tcaccgcgcg tcgccggaat gccaaaccag aacgagttcg
    56761 gccagaccag gcacgactga cgcagcgcca gaccagtgta ttcacgaaaa acctgaatat
    56821 catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc tgatcgtaaa
    56881 tctgctgcca ggcggtaatt gtcaaagcat gttcatagac gtccgttgtc tgtcgttgat
    56941 gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag ggttcatgat
    57001 aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg ggtgttgtag
    57061 gacacccggc acctccgaca ggttaatggg gcttgagacg gtaacgacta ctgcgtttac
    57121 gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc agcggccagc
    57181 caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt aacagcgcca
    57241 acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt acgggttgct
    57301 taggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc cgaccgatct
    57361 ccttgcggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg tccagcgccg
    57421 cacgacacgc cgccagatcg ccttccacca ccagcgttag gcgaccaggg tcaagcactt
    57481 cgtgactgag cagacgaacg ttagctgctt tgagcatggc atcagctgca tctatcgcgg
    57541 cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac ctccgttacg
    57601 cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg ttacaggctg
    57661 ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt tcagacggtg
    57721 gcgggacata ggtcaccagc tgcacgtcgg cagatttcaa cgctgcatca atgccgtagg
    57781 tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg atcccggcgg
    57841 ttgatgagag ataagaaccg gtacgcgaaa ctacatgtgc caggaaggct gtgttttctg
    57901 cgtcattagc ccactggaaa gcagcgccat tttcaatatt cgcaaccatc gcatccagac
    57961 cagcacgcac ttccgccggg tttggtccac caagcataat cagcacctca ccggcagtcg
    58021 gtgacgggcc gtgtgcagcg cccgcataca gcgagcgacc atataccact tcgaccatcg
    58081 cctgcttggt cgcttcgtca gccgcaatgt aggtgacgtc atctgaatca gcagaaatca
    58141 gcccgaggct acgaatatgc ggcggcaatt tcagttcacg tgcaaattcg gcgttaacgg
    58201 aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct ggcatgatgc
    58261 ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt ttcgccaaat
    58321 ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg ttagagatac
    58381 aagtgcggtc agcctcaacg gtggtcgcca tacgcggcga gtaaacggcg tagcaggaga
    58441 ggctttctga ctgccccaac cccggacgtt cgcccaccag caggatcact actttcgcgc
    58501 cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgtaca aagaatggcg
    58561 taccgacttt cagcccggct tgcttcaggc ccgccatcag cggcggcagg atctcttcgt
    58621 agttcacggt gatcgcatca gttgacaggc catcagaaat aacgacctgc acgtccggat
    58681 tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg cggcccatat
    58741 ccgggcgagt caggtagaga tttttgtcgc tgatttcgga gcggacttcc agcaagccct
    58801 gcgctttcac ccactcttcc ggtacttctt tcagaacggt atctttcgaa cgagagtgat
    58861 cggccaggaa acgcagcaac gcctgggtgc gcggacgcgg accggcacga ccggtacaaa
    58921 cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgtgatgc gggttttcaa
    58981 caccaatcca cgcttttgct tccgcagaac ctaaatccag cgcgcagctt tccgaggtca
    59041 ccggtgccgc acaggtggtg gtggcgcact ttgcttctga cggcgcgggg gccgcttgtc
    59101 ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc atgatgtgtt
    59161 atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca ggcgaccgtt
    59221 tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacgggc gcagattcag
    59281 taactgacgc acagtggcag tgtcgtggaa tgcggtggtc tgatagttga gcatgatgtc
    59341 atcgcccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca ggatcatcag
    59401 gttttcgttg aggttctggt cagcgtcagc gtggttggtg tagcagcagt cacagcccat
    59461 agagatgccg ctcagtttgc ccataaagtg atcttctaag cctgcgcgga taatctggcg
    59521 gtcgttgtag agatactccg gcccaataaa gccgaccaca gtgttgacga taaacggatc
    59581 gtaatgacgc gccagcccat agttacgcgc ttccatcgtc acctggtctg cgccgaagtt
    59641 cgcgccagca gacagcgccg aaccttgtcc ggtttcgaag tagaggcagt tttccccggc
    59701 gatacggttg aactccgcgc ccactgcgcg tgcttcatcg agcatcgcca gctctacgcc
    59761 aaactctttc agcccttttt cgctgccgca gatactctgg aaaatcagcc cgcctggtgc
    59821 gccgcgacga atcgcttcga tttgggtagt gacgtgcgcc agtatgcagc cctgagttgg
    59881 gatgttgaat ttgtcgatca cgccatagat ggtatccaac acgcggctta agttttccac
    59941 gtcgtcagtc accgggttaa cgccgatcac cgcatcgccc accccgaagg aaagcccttc
    60001 gtagatttgc gcggcgatac tttgcacgtc gtcacgggta tcgttcggct gcaagcgggc
    60061 gctgaaggtg cccggaatac cgatggtggt attggccttt ttgatcaccg gcattttctt
    60121 cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcca ccacttccga
    60181 ggtcagccct ttgcgggtaa aggcaatgtc atccacgctg gtttcatcgc tcagcacata
    60241 ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt cgttaacgtc
    60301 gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat tattgcggat
    60361 gtccgctaca gtcatttccg acaacacctg ctttgccgcc acgcgctcct gtgagcttgc
    60421 cgcagcaacg cccgccagca catcccccga acgcagttcg ttggctttag ccagcacctc
    60481 ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt tcataagtcg
    60541 ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga tccgccaaaa
    60601 agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc aatgactgcc
    60661 aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc aaagtcctgc
    60721 ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc gaccagcgcg
    60781 ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag cgccagcacg
    60841 tacgcgtcag ttttggggca aagatccagt tgaatcagcg cctgttgcca ggcactgacc
    60901 agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag ttgtacgccc
    60961 tccagccata ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc aatcaccgtg
    61021 gcgcgcacgg tttgcgccgg aaactgcacg ttcatctcac gcagacgcgg atggtcatgc
    61081 agcgccgttg ccagaagcgg gccgatatcg gcaaaacaga acgggtcggc gggctggtgg
    61141 cgataacatt cgcctacccc gccagaaagc gtaatgattt cgggcgtaac acctgcgggc
    61201 agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaga gcgttccgtc aatcacttcg
    61261 acaatcagtt ccgccatccg gcgggtaacc tgcgccaatt gcgcgccggt cagcgaacgg
    61321 gcgtcagtgc ctgggccgaa gcactcatcc acaatcatct gccccggttt atgagcgtaa
    61381 accacgcgcc cctggctgtc ggtttccagc aggcgaccgc cgacgttgag gcaggcagtg
    61441 ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc gatgtcgata
    61501 ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccggcacc gtgaccggcg
    61561 atcacggatt cgagatgcgg cccggcgctg gcgacgacaa aatcgcccag cgaccgagag
    61621 agcgccatca ccgccgggcg agcgttgcgg gttttcgcgc tttcaccggt gatgatgatg
    61681 gcaccagaat caacgctttc cggcgcaatg cccgcagcct gatattgctc gaggattaag
    61741 gttttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa gaacaccggg
    61801 ctttgccagc taatttcgcg tttgatgaat tcgtagcgcg gcacctgcga caccgccgca
    61861 cggttaacca gctccaggtg ggagaagatc acctgggtgg tggtggtgcc gatatcgata
    61921 ccgacgctca gtagctggcg agtgttcacg attgcgcctc cgcttcggtt ttagccgcgc
    61981 tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat tgccgttacg ccgccgatca
    62041 atttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg aagcctaaat
    62101 ggtcgcccag cgcgaaagca gcggaaacgg cgaaggcaca gttgatgact ttgccgcggg
    62161 tatccatctg cttcatcatg ccaaacatcg gaatgttgtt ggcaagcgtt gccaccatgc
    62221 cggctgccgc gatattgttc atattcagca ccttaccgac gctcatcagc ggtttttcaa
    62281 accagcgagt cagcagcagt accattggat acgcccctaa cagaacgcag gagatcgagc
    62341 cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc ataaagatag
    62401 gatcaaggcc cgggatcagt tcccagccaa ggaggaattt cactaccgca gcggcaagac
    62461 cgagggtgat caatgcaacg aggaatttgg cgaagatctg gaagccgttg atcatttttt
    62521 ccgggatgaa tttcagcccc agcgccacca gcaccgcaac gataagtacc gggatcatgt
    62581 tcatcaggat cagcgcaaag gtgaattcaa caggctggcc gttgatctgc acaccggagt
    62641 acatcgctac cagaccacca gcgatacagc caatcggaat ggtcactatg cccgccagca
    62701 cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc accggaatgg
    62761 aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat agccacgctg
    62821 ctacgtcacc gcccgccagc tctttggcga ggaagaagcc gcccatatcg cacgccagca
    62881 gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg ataatcaccg
    62941 gtccgagaac gtgggccagt accggtgcca gtgcggtcat accgaccata gccaggccca
    63001 gagcgcccat tgccataaag ccttcttcga actgaccgcc ggatccttcg atacttttac
    63061 cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg tctacggcag
    63121 ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc atcgccttta
    63181 ctccctgtta gttgttattt attggcggat gcggcgtgaa cgccttatcc gccctacatg
    63241 tgcaatcccg aaggccagat aagacgcggc aagcgtcgca tccggcaatt gcaccgcgcc
    63301 actggcggat gcggcgtgaa cgccttatcc gccctacatg tgcaatcccg taggccggat
    63361 aagacgcggc aagcgtcgca tccggcattt attgcgccgc tgcgtacagg ccgacaatct
    63421 gctcgaggct ggcggtacgc gggttactgc gcagacaaat atcttcctgc gcggcctgcg
    63481 cccatgcacc gtaatgcccg gttgttgcac ccacatcagc cagccgcttg ttgatcccaa
    63541 cttccgctat cagctcactt accgcattaa tagcgtcacg atcgtcggat tttttagttc
    63601 gcagcgcccg accaatctga ctaaaccgat cacgacaaac catccggtta aattccatca
    63661 ccgttggcag caacatggcg ttcgccagac cgtgcggaat atgcagcgcc gcccctggct
    63721 gatgcgccat cgcgtggcat aacccaagcc cggcgctgga aaacgccatt cccgccatac
    63781 atgaagccag caacatgctc tcgcgggcga caaggtcgtg accgtagccc accgctttcg
    63841 gcagcgattg accaatcatc gcaatggcgc caatcgccag gctgtcggta aacggtgtag
    63901 cgttcagcgc gctgtacgct tcaatggcat gggttaacgc atcaatgcca gtcatcgccg
    63961 tgacatgcga cggcacgcct tcggttagca cagcgtcgag aatcgcgaca tccggcatca
    64021 gcgaggcatg ggctaacacc tgtttgcgcc cgcttaccgc atcgataatc accgtcacgt
    64081 tggtggtttc agagccggtt ccggcggtcg ttggaatggc aatcaacggc aggcgcggtt
    64141 gcagaacgct ggtttctgac atttctgcca gcgtgctatc cgggttcgtc accagcaacg
    64201 ccacggcttt cgccgcatcc agcaccgagc cgccgccaaa cgcgatcacc ccgtcacaac
    64261 ctgattcacg caactgcgcc acggctgcac acacgtcggt gatgcacggt tcgcccaccg
    64321 gacatggcca gagcgtcatg gcgatacctt taacggccag gctacgcgtc agcccagcgg
    64381 tcatccctgc ctgatgcaaa aagctgtctg ccatcacgaa cagatgtttc aacccacgcg
    64441 tttgcgcttg ctgcccacaa ctgctcaccg cgcccggacc gcaaagcgtc accggtggaa
    64501 cgctaaatgt ttttacccgt tgcagattca gggtatcgaa cgcctgaaag agcgcggtct
    64561 gcaattcatt ttgcatatag cccctccgct ttctctcgcc cactactggc gattgccagc
    64621 ggggtcataa acaaactgtg ctgcggtaaa tgcacctgta acgccggaaa ttgtttgcaa
    64681 aacagcgccg ctacgcccgg ttgcatacag gaaccgcccg ccagccataa atccgtaatg
    64741 ccctgccctt caatatgacg ggccacaatg tccgccattt tttcgtacac cggtttcacc
    64801 gcgggccaaa tctcgtcacc gtgaccgcgt ttgtactgct ctgcctcttc cagcgaaata
    64861 cggcgatttc cggcgagagt cagagagatg tgatgtccgc cggtggcttc atccgccgag
    64921 tacgtcactt tgcccttttt cacgatggca atgccggtag tgccgccgcc gatatccact
    64981 acaccggcgt tgtccagttg cagcagatcc gcaactgcgg ttggctcatc cagcacatgg
    65041 ctcacttcca gtccggcaga ctccagcacg ttgatggaaa tacgcgggtc cgtaccagga
    65101 ggaaatgagg tcgccgcatg gctaaaacgg cgaccgaatt gctgctcaag ggtgtcgaga
    65161 tggcgacgaa caatggtgac cgcaccgaag aaatcccaga cgatgccgtc gcggaccaca
    65221 tcggcccagt cgaggcatac cgccaccggc tgaccgtcgc ggtcgacaac catcgacacc
    65281 acatcgcagg tgcccagatc cacccccagc cacagcggag attccgtcgc ggcgggcgtc
    65341 tggttacaca gcgttgccgc cgtttgcaga cgtggggtga gccattgttc atcgtgcgcc
    65401 atcggttact ccttaaacaa tgcgaaacgc atcgactaat acacagcgac gcagacggac
    65461 aaacgtccgc gcgctggtta ccccttcacc ggttggcgtg gtgatggtca tcgtggtcca
    65521 gccttccccg cccagcccca gcccggcaat acacggtccg ttcttaacga aaatgctggt
    65581 atcaatggcg ttcgccatct ggttcatgtt ttcgatattg cgcgagtgca ttgccgccgt
    65641 gtggtggcaa ccgccttcca gtttcaccgc cagcgcaatg gcatccgcca cgttggcgac
    65701 gcgcacgacg ggcagcaccg gcatcatcag ttcggtcacg gcaaacggat gttctgcggt
    65761 ggtttctaca aacagcagtc gcgtttgttc cggaacttta aggccgattg ccgccgcgat
    65821 tttcgccgca tcgcgaccaa cccagtcacg gctgacggtg ccttttccgc gctcgtcgat
    65881 atttttcagc aacaccgatt gcagttgctg cgcctgctct gcggtcagtt tcaccgcatg
    65941 ctggccttcc atcagacgca tcagttcatc ggctacgcta tcaacaacaa tcagtacctt
    66001 ttcgtcggca caaatgatgt tgttatcgaa agaagcgcct ttgacgatgg actgagcagc
    66061 acgggcgagg tcggcggttt catccaccac taccggcggg ttgccagcgc ctgcggcaat
    66121 cagacgttta ttggtgtgtt tacgcgccgc ttctactacc gcttcgccgc cggtcaccac
    66181 caaaaggccg atgcccggaa acttgaacaa gcgttgcgcg gtttcgatat ccggatttgt
    66241 tacggtaacc agtaagtttt ccggcccgcc tgcggcaaca atcgccaggt tgagcagcgt
    66301 aatcgcccgc tgggagactt ttttcgccgc cggatgcggg gcaaaaatga cgctgttgcc
    66361 cgcggcaatc aggctgatgg cgttgttaat tacggttgcc gccgggttag tggaaggcgt
    66421 cactgaagcc accacgcccc agggtgcgtt ttcaatcagg gtcgggccgt tgtcgccagt
    66481 cagcacttgc ggagagaggc actcaacgcc tggtgtgccg cgcgcctgag cgacgttttt
    66541 ggcaaattta tcttcaacgc gccccatgcc ggtttcactg acggcaagtt ccgctaaatc
    66601 tctggcgtgt ttttcgcctg cttcacgaat ggcagcaatg gctaactggc gcattgccac
    66661 gctttttaac ccttgctggg cgactttggc tgccgcaacg gcgtcatcca gggacgcgaa
    66721 aacgcccatc tcatgaacgg cggcgggcgg cgtgtcactg ctttgcattt tcagcagtac
    66781 cgctttcacc acctgttcaa tatcctgttg attcatgatg ttctgcctta tttgtggaaa
    66841 atgacctgac cgccagacac cacctcatcg acaatgccaa tcacgcacag atcgaccggt
    66901 gacgtttcgc ttttatgcgc ctggcgggcg gaactgccac tcaccagtaa cacccactcc
    66961 ccggttcccg cgccaatatt gtcgatggcg acggcgcatt gcccgtcagg attaccttgt
    67021 ggatcaatca tttccaccat cagcaatttg tcatgcgcca gtccgtgatg gcgtacggta
    67081 caaacaattt gtccagtgac gactgccagt ttcatacccg cctccgtggc gtatttcagg
    67141 taaaagctcc ccctaccctc cgcagaaggt aaaatgaaaa aggagagagc gtgacgcccg
    67201 aatcgacgtc acacagggtg attacaggtt gctgctatcg cctttcaggc cgatcgggaa
    67261 gacttcttcc aggtcgccgt gcggacgcgg gataacgtgt acagatacca gctcgccgat
    67321 acgctgtgcc gctgcggcac ctgcatcggt cgcggcttta caggctgcaa catcaccacg
    67381 aaccatggca gtacacaggc cgccgccaat ctgcttaaca ccaaccagct tgacgcgcgc
    67441 agctttaacc atcgcgtcag aagcctcaat cagtgcaacc aggccccggg tttcgatcat
    67501 tcctaatgct tccattgtgc tttcctcttt atcagggtcc agaacgggac cgttcattca
    67561 accagtgttt gtaaactgct ttcgcggttc acttctgtct gacgcggcac agctgccacc
    67621 agcgccagtt cgataatttc ctgcacgcta caaccacgag agagatcgtg catcggcgcg
    67681 gcaagtcctt gtatcaatgg cccgacggca cgatatccgc caagtcgttg tgcgattttg
    67741 tagccaatat ttccggcttc cagcgacgga aaaaccatca cattagcttt gccctgtaac
    67801 gggctggcag gcgctttttg cgccgccact tccggcacga aggcggcgtc aaactgcaac
    67861 tcgccatcga caaccagctt tggtgcgcgc tcacggacga tttctgtcgc ctgctggacg
    67921 ttggcaacac aggggtgacg ggcactaccg ttgctggaaa acgacagcat cgccacgcgc
    67981 ggttcttctc cggtgatggc gcgccaggtt tcggcgctgg caagcgcgat atccgccagt
    68041 tgtgccgccg tcggctgtgg caccacgctg caatcggcaa aacccaacgc cggaccgctg
    68101 tactgtggca gcatcaggaa aatggatgag agcgttttac agcctggctg caagccgata
    68161 atgcgtaatc cggcacgcag cacattcgcc gtggaagaga ggttgcccgc gatacagaca
    68221 tccgctttac cggcgctgac cattgcagcg gcgaacatca gcggatcggt aagtttttcc
    68281 agcgcatccg gcggcgtttt ttcgcccgcg cgggccagcc agcgatgagc aaattcttcc
    68341 cgcattgcga ggttgccatg cggatctatc acctgtagcc cgtccatcgc cacgccgtga
    68401 ctgagcgcaa actgacgaag ttcaaacgga ttggcgacca gaatgggcgt tgccagacct
    68461 tgttgatgta aatattgcgc agctttcagc acacgttgat ctaacgcatc cggaaaaact
    68521 actctggcgg gcgctcgcaa cgccagttca cgacaacgtt taataatcat tgcgtttccc
    68581 ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt cgttacgctc aggatcatca
    68641 tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa aatgtccggg cgtaatactt
    68701 caaagctgcg ggtgataaag acctgcgccg ctacggtttc tgtttcccgc actttggtgc
    68761 gtagcagatt gagcaacgcc gcatcgcgcc cgtggctggc ttccggcacc agatgatcgt
    68821 ggtcgagata acgcagcggc tggtgagaaa gccgatgcag atcttcgtca gaaagcccga
    68881 caatcgcttg ctcgcccagc ggttccccca gcgcatcggc acgcataatg ttgccaagac
    68941 gcgagcggat gtccgccagc cacggctgcc acggttctgc cagttcaatt tgcagccaca
    69001 ctgccagcgc aatggtgctg tcgagaacag cgcgaaaacc cagacgcgga tcgcttttgg
    69061 caaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc accggctggc
    69121 ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggtcagcccg tgaacgggct
    69181 gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata aacttgatgc
    69241 gcagatggcg gctttccagt aactcccggg cagagggcgt caggcgactg tccgcaggga
    69301 gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct tcggtgatga
    69361 aatctttcat aaggattgcc agttagccgg ccaggcgaca tacaggaatt tcacgctgga
    69421 tgtcgtacca aattcgatgc tggagccttt cgggataaac atcacatcgc ccgctttggc
    69481 gatcatagtt tcgccttcgt ggcgaacatg cagctcccct tccagcacca tgtcgatttc
    69541 gtcgtagttc agggtccacg gaaagaaggc gttctcccac tgcatgaaac cagcggccat
    69601 actgctgccg tcgtctccgg tcacgagatc agtcaagcct acgcagtgcg gttccgcgcc
    69661 atcgaaacga ccaaacttga cgctgctgcc atcaataact ttgatgccgc ctttgccggt
    69721 caccgattta aagctcggct gcagtgcacc ctgttccagc gactgttttt ccttcatcac
    69781 tttttccatc agctgcgcga ccaggctttc ggtaaactga ccttccggca gttgggcgat
    69841 aatggtttca cggatgcgct ggctttcggt tttatcggct gatgcgctgg cgggaacaga
    69901 cgccgttacc gggatcgatt catcacattc ggtgatggta aaacccaaca gatcagccac
    69961 ttcacgagct tccggggtaa taatgctggc gcgcagaacc acggacattg ccagttcgcc
    70021 gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct gcttcctcct
    70081 gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg tcgtggctgt
    70141 ttagctcgaa aatcggctct tcgaagcccg tctcaagcaa taactttcgc gttgcggcga
    70201 cgtcagcatc tggcatgtcc gttttactga tgacggcgat ttgccgtttg ctgacgccaa
    70261 tatccagcaa cccggcaggc aaccgacttt ccgggtcatt cgcgccgtgg acatagatca
    70321 gcatatccac atcctgtagc gtggtaatta aggcgtgata ccagcgggga tggctaaaat
    70381 attcacccgg cgtatcaata tcgccattat cattaaattc cacggcctgt gtttttcttg
    70441 cgagggtata attcccctgc aacgcattaa ataacgttgt ttttcctgcc ccgacggtac
    70501 ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt tcacaaagcg
    70561 tataatttaa taagcgtcct aaaccactga cggtttgtga taacgcttct tctactgcgc
    70621 ccaccgagcc ataaatcacc agcgcgccac taaaacgatc gagaaaaccg atatgcacat
    70681 cggcggcttt caacgctaaa tcaccggcga tcatcgccgt ttctccggga gttagcgtca
    70741 taatgccgat tgcgcccgca tccggaacgc cgattttctt cgccagttcc tcgccgggat
    70801 gcgcgatgag atgcgccagt gtgacctgtt taccgggcac aaattcctga atgatgcgtt
    70861 ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta tcgtgacaat ttttcgtcag
    70921 agtaaataac aaaattcggc aagcgcgttt aaaaggtgag gtagatcact aagaatggag
    70981 agatatttgc tgtggt
//
LOCUS       DAPQZD010000005        63792 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPQZD010000005 DAPQZD010000000
VERSION     DAPQZD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.005"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            221..1309
                     /gene="ompF"
                     /locus_tag="RO454_000342"
     CDS             221..1309
                     /gene="ompF"
                     /locus_tag="RO454_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDY0932226.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            1494..2684
                     /gene="aspC"
                     /locus_tag="RO454_000343"
     CDS             1494..2684
                     /gene="aspC"
                     /locus_tag="RO454_000343"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDY0932227.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(2734..3381)
                     /gene="gloC"
                     /locus_tag="RO454_000344"
     CDS             complement(2734..3381)
                     /gene="gloC"
                     /locus_tag="RO454_000344"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDY0932228.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3408..3956)
                     /locus_tag="RO454_000345"
     CDS             complement(3408..3956)
                     /locus_tag="RO454_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDY0932229.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(4137..5984)
                     /gene="ldtD"
                     /locus_tag="RO454_000346"
     CDS             complement(4137..5984)
                     /gene="ldtD"
                     /locus_tag="RO454_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDY0932230.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(6245..10705)
                     /gene="mukB"
                     /locus_tag="RO454_000347"
     CDS             complement(6245..10705)
                     /gene="mukB"
                     /locus_tag="RO454_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDY0932231.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVWKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(10705..11409)
                     /gene="mukE"
                     /locus_tag="RO454_000348"
     CDS             complement(10705..11409)
                     /gene="mukE"
                     /locus_tag="RO454_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDY0932232.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(11390..12712)
                     /gene="mukF"
                     /locus_tag="RO454_000349"
     CDS             complement(11390..12712)
                     /gene="mukF"
                     /locus_tag="RO454_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDY0932233.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(12709..13494)
                     /gene="cmoM"
                     /locus_tag="RO454_000350"
     CDS             complement(12709..13494)
                     /gene="cmoM"
                     /locus_tag="RO454_000350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDY0932234.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            13630..14409
                     /gene="elyC"
                     /locus_tag="RO454_000351"
     CDS             13630..14409
                     /gene="elyC"
                     /locus_tag="RO454_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDY0932235.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(14386..15279)
                     /locus_tag="RO454_000352"
     CDS             complement(14386..15279)
                     /locus_tag="RO454_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDY0932236.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(15433..16179)
                     /gene="kdsB"
                     /locus_tag="RO454_000353"
     CDS             complement(15433..16179)
                     /gene="kdsB"
                     /locus_tag="RO454_000353"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDY0932237.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(16176..16358)
                     /gene="ycaR"
                     /locus_tag="RO454_000354"
     CDS             complement(16176..16358)
                     /gene="ycaR"
                     /locus_tag="RO454_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDY0932238.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            complement(16410..17642)
                     /locus_tag="RO454_000355"
     CDS             complement(16410..17642)
                     /locus_tag="RO454_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDY0932239.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            complement(17679..18665)
                     /gene="lpxK"
                     /locus_tag="RO454_000356"
     CDS             complement(17679..18665)
                     /gene="lpxK"
                     /locus_tag="RO454_000356"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDY0932240.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(18662..20410)
                     /gene="msbA"
                     /locus_tag="RO454_000357"
     CDS             complement(18662..20410)
                     /gene="msbA"
                     /locus_tag="RO454_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDY0932241.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(20447..22711)
                     /locus_tag="RO454_000358"
     CDS             complement(20447..22711)
                     /locus_tag="RO454_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDY0932242.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(22917..23201)
                     /gene="ihfB"
                     /locus_tag="RO454_000359"
     CDS             complement(22917..23201)
                     /gene="ihfB"
                     /locus_tag="RO454_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDY0932243.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(23361..25034)
                     /gene="rpsA"
                     /locus_tag="RO454_000360"
     CDS             complement(23361..25034)
                     /gene="rpsA"
                     /locus_tag="RO454_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDY0932244.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(25145..25828)
                     /gene="cmk"
                     /locus_tag="RO454_000361"
     CDS             complement(25145..25828)
                     /gene="cmk"
                     /locus_tag="RO454_000361"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDY0932245.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(26001..26783)
                     /gene="ycaL"
                     /locus_tag="RO454_000362"
     CDS             complement(26001..26783)
                     /gene="ycaL"
                     /locus_tag="RO454_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDY0932246.1"
                     /translation="MYLKSNQKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNA
                     YKAATLSDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYK
                     VYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAI
                     VAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQ
                     GLVGSFEKLASLDGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(26927..27316)
                     /locus_tag="RO454_000363"
     CDS             complement(26927..27316)
                     /locus_tag="RO454_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDY0932247.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPEVADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGKLFADEPDADTEKKE"
     gene            complement(27288..27737)
                     /locus_tag="RO454_000364"
     CDS             complement(27288..27737)
                     /locus_tag="RO454_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDY0932248.1"
                     /translation="MQKKRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKKKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLSTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(27739..27945)
                     /locus_tag="RO454_000365"
     CDS             complement(27739..27945)
                     /locus_tag="RO454_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932249.1"
                     /translation="MTTETIVCFLFWYMYAGSCSARLHRSLGYGRHYDTAHYILYMTA
                     VMLFWPVTLPAATDIVADRLKKRR"
     gene            complement(27935..28165)
                     /locus_tag="RO454_000366"
     CDS             complement(27935..28165)
                     /locus_tag="RO454_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932250.1"
                     /translation="MITLSGKSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHIEWGGIQNDY"
     gene            complement(28162..28845)
                     /locus_tag="RO454_000367"
     CDS             complement(28162..28845)
                     /locus_tag="RO454_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDY0932251.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAAYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(28842..29057)
                     /locus_tag="RO454_000368"
     CDS             complement(28842..29057)
                     /locus_tag="RO454_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932252.1"
                     /translation="MADILREITAWTLILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            complement(29072..29368)
                     /locus_tag="RO454_000369"
     CDS             complement(29072..29368)
                     /locus_tag="RO454_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932253.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLAEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            complement(29378..29650)
                     /locus_tag="RO454_000370"
     CDS             complement(29378..29650)
                     /locus_tag="RO454_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932254.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMATQA
                     IVATNGKEAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            complement(29939..30469)
                     /locus_tag="RO454_000371"
     CDS             complement(29939..30469)
                     /locus_tag="RO454_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDY0932255.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
                     SDIEDFSIIPFEQDVQ"
     gene            complement(30497..30766)
                     /locus_tag="RO454_000372"
     CDS             complement(30497..30766)
                     /locus_tag="RO454_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932256.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            complement(30769..31935)
                     /locus_tag="RO454_000373"
     CDS             complement(30769..31935)
                     /locus_tag="RO454_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0932257.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDAGA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            complement(31946..33715)
                     /locus_tag="RO454_000374"
     CDS             complement(31946..33715)
                     /locus_tag="RO454_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDY0932258.1"
                     /translation="MTPALTEKLVETARAARDAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSALTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFITAGRTDETTSEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVAAPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQMAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDTTLPAYMP
                     KRGQASDVRGPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            complement(33731..34048)
                     /locus_tag="RO454_000375"
     CDS             complement(33731..34048)
                     /locus_tag="RO454_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932259.1"
                     /translation="MNTNNTQNTLMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQETHPFVLTMEVMLMQQIRRLQNQSALRPVVAPQPVNTVVKTCDGETLCDLARKIAA
                     RIG"
     gene            complement(34048..34968)
                     /locus_tag="RO454_000376"
     CDS             complement(34048..34968)
                     /locus_tag="RO454_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDY0932260.1"
                     /translation="MGRTKSPINTELNADVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERERIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFTNILENDLGLAPQVARR
                     MMQASVKFLGNGDEQPKRSALSVLGKTKLYELMVLDDEELDALADGGTVAGATLDDID
                     RMTSRELKAALREARETNAAQQQVLAGKDEKINELATKLEKKSRLQPPPPDEELKKLR
                     AEVTALAVEAESAIAVRLSSVFETLCEYCTKNMIDTPRDFMAGLVCQIERSVHALRET
                     FDLEAAPSGNDAPAWLTEPEPEINRPEDMQ"
     gene            complement(34979..35287)
                     /locus_tag="RO454_000377"
     CDS             complement(34979..35287)
                     /locus_tag="RO454_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0932261.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELATALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            complement(35340..35528)
                     /locus_tag="RO454_000378"
     CDS             complement(35340..35528)
                     /locus_tag="RO454_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDY0932262.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQTKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            35622..35978
                     /locus_tag="RO454_000379"
     CDS             35622..35978
                     /locus_tag="RO454_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0932263.1"
                     /translation="MSKDIGERVISVRKKAGLNQREFAARLGISNGGISQIENGKAMP
                     GGEFLLKMHQEFNVDVNWLLTGMSTDGMTRNQNQISPEKQKLIDTFDDMTPDQQRSFL
                     EIGRFYTQPKPGKIAG"
     gene            complement(36095..36859)
                     /locus_tag="RO454_000380"
     CDS             complement(36095..36859)
                     /locus_tag="RO454_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDY0932264.1"
                     /translation="MKMQTLPIVPWIGGKRRLAKHILPLFPAHECYVEPFCGAAALYF
                     LKTPGKIEVINDINGELVNLYRVVKHHLEEFVRQFKWALVSRQIYKWLQITPEETLTD
                     IQRAARFYYLQKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTVIEHM
                     DWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIISVNDI
                     PQMREVFTGLNIQTVNINYSLAGKPTPRRELLICNF"
     gene            complement(37050..37265)
                     /locus_tag="RO454_000381"
     CDS             complement(37050..37265)
                     /locus_tag="RO454_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932265.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLYRGELSVCRAAFHFQGI
                     QKQFDTSVFAEGITYALDRIRSENRPG"
     gene            37267..37668
                     /locus_tag="RO454_000382"
     CDS             37267..37668
                     /locus_tag="RO454_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932266.1"
                     /translation="MHSLNDDFYVLTGKIFCRLLNTHPAPQVLIYSGFFDIPDQVDHD
                     QKKCRCFIDTLIWLRDNSYIRYSQLLAVGAADVVLTEKTLCLLNAMPTFLQPRPVPFK
                     ESLLDAVKTGENAVIAECVSRIMRYVFNERK"
     gene            complement(37644..38372)
                     /locus_tag="RO454_000383"
     CDS             complement(37644..38372)
                     /locus_tag="RO454_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932267.1"
                     /translation="MTQTLNPVITVTVSGPVGSGKSYVLARIEEVVKQEFGNSVIVDA
                     ADVDGERRMSGDDLSTWQKPRAGTVIRLEEYTGQGLLSYGEERICRTNELLDVICPTG
                     RQPWILPDALHATIAALMTVYDQELLMKELRKLTGQSTECCSSPKYGKQEHIPINQPP
                     RFFDEYLLTPENKAEHEEIKAALFREIRKIAEMQLEGGLDRVMARGEWASLFERVSPE
                     LLPLALAIGIMQTADKPHLRSLNT"
     gene            38503..38853
                     /locus_tag="RO454_000384"
     CDS             38503..38853
                     /locus_tag="RO454_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HDY0932268.1"
                     /translation="MKNLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHDDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            38856..39596
                     /locus_tag="RO454_000385"
     CDS             38856..39596
                     /locus_tag="RO454_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDY0932269.1"
                     /translation="MNWPQITWIVCMSLKLAFEAFRVFIRTERLSVRAGTFLVHMAWV
                     FFVAMLLWCGGFFSQACAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRPDAISPAGAQGLAQFMPATADWISQLIPMLSSREPFNPAWAIRALVSYDRWLWQRV
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILRELAPRYLTWGGSSCVASG"
     gene            39580..40230
                     /locus_tag="RO454_000386"
     CDS             39580..40230
                     /locus_tag="RO454_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932270.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLVGLYAMGYRSGHDSAKRDGDT
                     ALSQLQSAFDAYKTEQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAWQCRRWRNQINALLDEREGLQK"
     gene            40227..40553
                     /locus_tag="RO454_000387"
     CDS             40227..40553
                     /locus_tag="RO454_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932271.1"
                     /translation="MTLQVEFWTVVSFLLTFMRFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            40553..40864
                     /locus_tag="RO454_000388"
     CDS             40553..40864
                     /locus_tag="RO454_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932272.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVELEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            40864..41409
                     /locus_tag="RO454_000389"
     CDS             40864..41409
                     /locus_tag="RO454_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDY0932273.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            41460..43001
                     /locus_tag="RO454_000390"
     CDS             41460..43001
                     /locus_tag="RO454_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932274.1"
                     /translation="MSPMDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIE
                     KSRRTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAA
                     SEIEEGFWEEDDDDKHIKTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQ
                     LDELLKAALAMLIWGGKVRVISTHDGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSE
                     GLFRRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESR
                     MSADTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRS
                     GDLSVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNG
                     AYLAEYAMQRYGSGRVQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAV
                     QVVNGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAAR
                     MLEGF"
     gene            43001..44497
                     /locus_tag="RO454_000391"
     CDS             43001..44497
                     /locus_tag="RO454_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDY0932275.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRSWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELRAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETPPSERQNNMLPLSFAEAIDADIQAQQQLDDALDILMNGGALNGTLEPVLAPLFRRV
                     ENGVNPSELLGELAELYPQMNTDDLQERLARILFVANIRGRLHERDNG"
     gene            44478..45299
                     /locus_tag="RO454_000392"
     CDS             44478..45299
                     /locus_tag="RO454_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDY0932276.1"
                     /translation="MSVTTAELAYCMTLPPKRAINYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQELEPVLKRKGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            45302..45760
                     /locus_tag="RO454_000393"
     CDS             45302..45760
                     /locus_tag="RO454_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDY0932277.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERDGQTLQDTGRLMGSVSTDHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            45975..47090
                     /locus_tag="RO454_000394"
     CDS             45975..47090
                     /locus_tag="RO454_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDY0932278.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            47105..48058
                     /locus_tag="RO454_000395"
     CDS             47105..48058
                     /locus_tag="RO454_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932279.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            48068..48406
                     /locus_tag="RO454_000396"
     CDS             48068..48406
                     /locus_tag="RO454_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDY0932280.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            48408..48854
                     /locus_tag="RO454_000397"
     CDS             48408..48854
                     /locus_tag="RO454_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDY0932281.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            48854..49318
                     /locus_tag="RO454_000398"
     CDS             48854..49318
                     /locus_tag="RO454_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDY0932282.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            49318..49569
                     /locus_tag="RO454_000399"
     CDS             49318..49569
                     /locus_tag="RO454_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932283.1"
                     /translation="MKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVAL
                     KHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            49559..50986
                     /locus_tag="RO454_000400"
     CDS             49559..50986
                     /locus_tag="RO454_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDY0932284.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            50983..51507
                     /locus_tag="RO454_000401"
     CDS             50983..51507
                     /locus_tag="RO454_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDY0932285.1"
                     /translation="MMSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKAL
                     GMVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDE
                     IPLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVG
                     GVDQLTDYRINIGG"
     gene            51510..51791
                     /locus_tag="RO454_000402"
     CDS             51510..51791
                     /locus_tag="RO454_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932286.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGIGKSEGKTLLIAFLRNCKGFRTC"
     gene            51889..52224
                     /locus_tag="RO454_000403"
     CDS             51889..52224
                     /locus_tag="RO454_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDY0932287.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            52148..52306
                     /locus_tag="RO454_000404"
     CDS             52148..52306
                     /locus_tag="RO454_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDY0932288.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            52382..55333
                     /locus_tag="RO454_000405"
     CDS             52382..55333
                     /locus_tag="RO454_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDY0932289.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAMGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQDLLPLIQGTKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVNVWKTAVLLRTNWHRLTTALGEGGKLRWLVTGFSRLTSGGLKLSKVLAGSLVRGF
                     MSAARAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGAVSGWFKQRWADIQEAFNGG
                     IAGTGKLLINWSPAGLLYKAFAAALKYFGVTLPAKFTDFGGHLIDGLINGIKNKWGSL
                     KSSVTGMGDSISSWFKQCWADIQEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLG
                     VDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFM
                     GFGDNIAQGAAIGLQRTTPLAALAGQRMASELLPRMPVSIQGPEIRDNTSGVRFSMPL
                     PDINGFMSSAKNAIGAVINSLSSMPAIPLSTRASILPGQRLANEMTPDVPRIPSPEIL
                     AAGYSGRGAAATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLHELEKMLER
                     LLAQKQRKEYR"
     gene            55333..56217
                     /locus_tag="RO454_000406"
     CDS             55333..56217
                     /locus_tag="RO454_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0932290.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQITLVFHQHYCVPDVELARLRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAVGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            56217..56429
                     /locus_tag="RO454_000407"
     CDS             56217..56429
                     /locus_tag="RO454_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDY0932291.1"
                     /translation="MFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVLP
                     SGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            56417..57571
                     /locus_tag="RO454_000408"
     CDS             56417..57571
                     /locus_tag="RO454_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDY0932292.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVSFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            57568..58164
                     /locus_tag="RO454_000409"
     CDS             57568..58164
                     /locus_tag="RO454_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDY0932293.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLIIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGEGVTATIHGNIEIKGNVHATGSMLSDGENSSHHSH"
     gene            58219..58566
                     /locus_tag="RO454_000410"
     CDS             58219..58566
                     /locus_tag="RO454_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDY0932294.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            58557..59660
                     /locus_tag="RO454_000411"
     CDS             58557..59660
                     /locus_tag="RO454_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDY0932295.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTATCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVNLLVSNLMASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMTGVSDD"
     gene            59653..60231
                     /locus_tag="RO454_000412"
     CDS             59653..60231
                     /locus_tag="RO454_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDY0932296.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            60234..61481
                     /locus_tag="RO454_000413"
     CDS             60234..61481
                     /locus_tag="RO454_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDY0932297.1"
                     /translation="MGNLNETEKWEENIYQLETSDPVLGGADGISNRAPRQLANRTKW
                     LKKKTEEAAQSLAEHVRSRNHPDATLTAKGFTQLSSATNSTSETLAATPKAVKAAYDL
                     AAGKAPASHTHPWSQITGVPAASLTAKGTVQLSSATDSQSETEAATPKAVKAAYDLAA
                     GKAPVSHTHPWSQITGVPAASLTAKGTVQLSSAINSTSEILAATPKAVKAAYDLANGK
                     QPADATLTALAGLATAADRLPYFTGADRAELATLTAIGRAIIAKGSIKDVLNYLGLGE
                     GSALPVGVPVPWPTATPPAGWLKCDGRAFTKEQYPVLARVYPTLRLPDLRGEFIRGWD
                     DGRKVDTGRDLLSRQDGTSFSHYAGNFDIGSGHSINNYDQIVDNQPGFSRFSFAGPSR
                     GDGVNYVTIRPRNIAFNYIVRAA"
     gene            61492..61953
                     /locus_tag="RO454_000414"
     CDS             61492..61953
                     /locus_tag="RO454_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDY0932298.1"
                     /translation="MGEGSALPVGVPVPWPTATPPAGWLQCNGATFTKEQYPVLVRVY
                     PTLRLPDLRGEFIRGWDGGRKVDTGRALLSFQEGTIVSGFDDNDTGDISSLGSTQYGF
                     GDALTSNQLGVLNGKKWIFDASSKGAQRYDWWAYVSARPRNIAFNYIVRAA"
     gene            complement(61960..62574)
                     /locus_tag="RO454_000415"
     CDS             complement(61960..62574)
                     /locus_tag="RO454_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0932299.1"
                     /translation="MSNTAVLDENGIATVAGDITVYHYDEETREYTSSSVEYLALGVG
                     TPAHSCADAPPEAISGYVVCRTATLNGWEHVPDHRGETVYSTENGNPVLITQPGDYPA
                     DTTTKQPATPWDTWNGEAWVTDTERQRAAELEVARQQRQQRVKQAMASVDLINLKLRA
                     GRSLKPEETAKLNAVLDYIDELNALDISKAPEISWPEAPLALAG"
     gene            complement(62574..63098)
                     /locus_tag="RO454_000416"
     CDS             complement(62574..63098)
                     /locus_tag="RO454_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDY0932300.1"
                     /translation="MNVGLGEGSALPVGVPVPWPTATPPAGWLKCDGRAFTKEQYPVL
                     ARVYPTLRLPDLRGEFIRGWDDGRKVDTGRKLLSAQGATLLRTAMLDYYNQDTTGTSG
                     IVGMGFNNEDSITDLREGSFKMPDGTTFSDPVVAMSDNGMQATILTSIRSGYAKGITV
                     RPRSIALNYIVRAV"
     gene            63158..63730
                     /locus_tag="RO454_000417"
     CDS             63158..63730
                     /locus_tag="RO454_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0932301.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMERELIVERTLAGLAAARAQGRVGGRRPKLTKEQHEQIARLLQKGYD
                     RKRLAIIYDIGLSTIYRYHPVGTVITQPEM"
BASE COUNT        14636 a        17158 c        16941 g        15057 t
ORIGIN      
        1 ttccttaatt tttacttttt gtcacacatt ttttcttttt gaaaccaaat ctttatcttt
       61 gtagcacttt cacggtagcg aaacgttagt ttgaatggaa agatgcctgc agacacataa
      121 agacaccaaa ctctcatcaa tagttccgta aatttttatt gacagaactt attgacggca
      181 gtggcaggtg tcataaaaaa aaccatgagg gtaataaata atgatgaagc gcaatattct
      241 ggcagtgatc gtccctgctc tgttagtagc aggtactgca aacgctgcag aaatctataa
      301 caaagatggc aacaaagtag atctgtacgg taaagctgtc ggtctgcatt atttctccaa
      361 gggtaacggt gaagacagtt acggtggcaa tggcgacatg acctatgccc gtcttggttt
      421 taaaggggaa actcaaatca attccgatct gaccggttat ggtcagtggg aatataactt
      481 ccagggtaac aactctgaag gcgctgacgc tcaaactggt aacaaaacgc gtctggcatt
      541 cgcgggtctt aaatacgctg acgttggttc tttcgattac ggccgtaact acggtgtggt
      601 ttatgatgca ctgggttaca ccgatatgct gccagaattt ggtggtgata ctgcatacag
      661 cgatgacttc ttcgttggtc gtgttggcgg cgttgctacc tatcgtaact ccaacttctt
      721 tggtctggtt gatggtctga actttgctgt tcagtacctg ggtaaaaacg agcgtgacac
      781 tgcacgccgc tctaacggcg acggtgttgg cggttctatc agctacgaat acgaaggctt
      841 tggtatcgtt ggtgcttatg gtgccgctga ccgtaccaac ctgcaggaag agtctcccct
      901 tggcaaaggc aaaaaagctg aacagtgggc tactggtctg aagtatgacg cgaacaacat
      961 ctacctggca gcgaactacg gtgaaacccg taacgctacg ccgatcacta acaaatttac
     1021 aaacaccagc ggcttcgcca acaaaacgca agacgttctg ttagttgcgc aataccagtt
     1081 cgatttcggt ctgcgtccgt ccatcgctta cactaaatct aaagcgaaag acgtagacgg
     1141 tatcggtgat gttgatctgg tgaactactt tgaagtgggc gcaacctact acttcaacaa
     1201 aaacatgtcc acctatgttg actacatcat caaccagatc gattctgaca acaagttggg
     1261 cgtaggttcc gacgataccg ttgctgtggg tatcgtttac cagttctaat agcacacctc
     1321 tttgttaaat gccgaaaaaa caggactttg gtcctgtttt ttttatgcct tccagagcaa
     1381 tctcacgtct tgcaaaaaca gcctgcgttt tcatcagtaa tagttggaat tttgtaaatc
     1441 tcccgttacc ctgatagcgg acttcccttc tgtaaccata atggaacctc gtcatgtttg
     1501 agaacattac cgccgctcct gccgacccga ttctgggcct ggccgatctg tttcgtgccg
     1561 atgaacgtcc cggcaaaatt aacctcggga ttggtgtcta taaagatgag acgggtaaaa
     1621 ccccggtact gaccagcgtg aaaaaggctg aacagtatct gctcgaaaat gaaaccacca
     1681 aaaattacct cggcattgac ggcatccctg aatttggtcg ctgcactcag gaactgctgt
     1741 ttggtaaagg tagcgccctg atcaatgaca aacgtgctcg cacggcacag actccgggtg
     1801 gcactggcgc actacgcgtg gctgccgatt tcctggcaaa aaataccagc gttaagcgtg
     1861 tgtgggtgag caacccaagc tggccgaacc ataagagcgt ctttaactct gcaggtctgg
     1921 aagttcgtga atacgcttat tatgatgcgg aaaatcacac tcttgacttc gatgcactga
     1981 ttaacagcct gaacgaagcc caggctggcg acgtagtgct gttccatggc tgctgccata
     2041 acccaaccgg catcgaccct acgctggaac aatggcagac actggcacaa ctctccgttg
     2101 agaaaggctg gttaccgctg tttgacttcg cttaccaggg ttttgctcgg ggtctggaag
     2161 aagatgctga aggactgcgc gctttcgcgg ctctgcataa ggagctgatt gttgccagtt
     2221 cctactctaa aaactttggc ctgtacaacg agcgtgttgg cgcttgtact ctggttgctg
     2281 ctgacagtga aactgttgaa cgtgcattca gccaaatgaa agcggcgatt cgtgctaact
     2341 actctaaccc accagcacac ggcgcttctg ttgttgccac catcctgagc aacgatgcgt
     2401 tacgtgcgat ttgggaacaa gaactgactg atatgcgcca gcgtattcag cgtatgcgtc
     2461 agttgttcgt caatacgctg caggaaaaag gcgcaaatcg cgacttcagc tttatcatca
     2521 aacagaacgg catgttctcc ttcagtggcc tgacaaaaga acaagtgctg cgtctgcgcg
     2581 aagagtttgg cgtatatgct gttgcttctg gtcgcgtaaa cgtggccggg atgacaccag
     2641 ataacatggc accgctgtgc gaagcgattg tggcagtgct gtaagcgtta aaaccgatga
     2701 agcccgcttg aaagcgggct tttttatgcg cctttaccag acaggcattt cgtcttgcaa
     2761 gaagggatta tgcaggcgct cataaccgag agtggataat ggtccgtgac ccggaataaa
     2821 tgtcacgtca tcccccagtg gcagcaattt atctttgatt gaagaaatca gttgattgtg
     2881 atcgccacgc gggaaatcac tgcgccctac tccgcctttg aaaataacat cgccagaaat
     2941 gagcagcctt gcctgctcat taaaaaacac cacatgaccc ggcgtatgcc cggggcaatg
     3001 taacacctgt aaagtcacat tccctatgct gatggtatcg ccttcgttca gccaacgatc
     3061 tggcgtcagc ggctggcact cttccagacc aaacatacga ctttgcgcag gcaagccttg
     3121 cagccagaac tcatcttctt tttccgggcc aaaaaccggc acgccgtaat gttgcgccag
     3181 ttccgccgct gcgccaacgt ggtccagatg accatgagtc agcaggatct gcatcagtgt
     3241 caggccgctg gcatcaactt cctgtttgat tttttccgca tcgccgccag gatcgaccag
     3301 tgcggccaga cgggtttgtt cacaccagat caatgaacag ttctgggaga atgcggtgac
     3361 cggaataata cgatagttca tactgcccct gtttcgttaa gcgattacta ccagtgccgt
     3421 gctggcccgg tatcaatatg cacaaagtta ctacgtgggt aatatcctac accacctgcg
     3481 cgcatagata acgcggcttt gcgaatattg cttaacgcga taccttcaat gtggaaatcc
     3541 atcgcctggc ctttggtgtg atagcttttc ttcgctactc cacggctgcg ggcgcgtagt
     3601 tcattgttgg tatcaatgga acgataaccg gaaatgagtt gcaccggttt gcgggtgcct
     3661 aacaaccctt gcaggcgata caactggtcg aataatcctg ggtcgatgga ctttattttg
     3721 ttcgcgcggt aatcgcggaa aaaatggtta agttttgcca attcttcctg aatatagcct
     3781 ctgccatcga aaaactccgc tttgattgac tctccggtat gaagattatt gagtgtcaaa
     3841 atgcgcgggc gtggggtaga gagtgttgca aacgccgggg tcggcaggat ggcggcaccg
     3901 agtgcaacgc caccaagcgc cagcagtttg cggcgattag cgtcgaattt gtccatgata
     3961 atcaagtcta caggtcaatg ttatcgttta tatgcacttc tggcgcacaa gaggcacctt
     4021 aaccgcccgt aaagaagacg tcaagatgac tcaaccccag cagtgacggg ggctgcagag
     4081 aatcgccccc gatcatgcta attattacga caactgattt ccccgaactg cttcatttac
     4141 ctgattaatt gttccgcttt cgatacaatt tgcgagctgg atcgcgcagg cagatcataa
     4201 ttgtaaatat ctgtacgata ctgggtacga ccatcagcac caacaaaggc cgtcagatag
     4261 tagagattca ccggaatcga ctgccgaata ttgacgtaac gcgtatcgcc ctgcttcagc
     4321 gcatcagaaa tacgtttgtc attccatcct gcatcctgca acagcatatt cgccagatcg
     4381 gaagctttat tcactcgtac acagcctgag ctcaatgcgc gtgtatcacg tttgaacaga
     4441 ttgtggttcg gcgtgtcatg caaataaatg gcctctgaac tcggcatatt gaatttatag
     4501 cgccccagcg agttccgtgg gcctggagcc tgctggaaac ggaacggtaa attcgaggcc
     4561 gtgattgtag accagtcaac ctgccatggg tcaatcgctt ctctgctgtt ccagccgcgc
     4621 atcaccgtat agccatggct ttcgagatat cccggatcgt tgcgcacttt cggcagaata
     4681 tctttgcgtg ccagagttgg tggtacgttc cacggcgggt ttaccactac gttgttaagt
     4741 gcactgctca tcatcggcgt tttgcgatcg gggcgaccga caatgactcg cgaatccagc
     4801 acctgattgc cgttctgata gtagaccagc gaataggctg gaatgttaac catgatcccg
     4861 gtagaaagct ctgttggcag caagcgcaat cgctggatgt tgagtgccaa cacgccagca
     4921 cgctgggcgg gcgttacgtt taaccagtca cgcgttgccg ggccaatagc accatctgct
     4981 cccaatcctt gccatgcctg aaaacgttta acggcttcca ccagttcatt atcgtaggcg
     5041 gcgcgaacgg caggcgcagg tttactacga gacgtagttt gtttgtccat cagcttagtt
     5101 tctgctgttt caacagtaac agccgatggg ctgactaccg cgtcagttgg cgtgtcatcg
     5161 ccaggtagcg taattttcgg tcccccgtcc aacatgcctg tacgttgcaa tatttcgcgc
     5221 aacgccggta cgtcgttact ccactgtcct gggcgcaacg ttgctttacc ggtcagttga
     5281 ggccacggtt tggtgtcact gagtaaggcc agtaacgatt catgcatcgc cgcatattgc
     5341 ggatgctgcg gtgccagtcc tgcaacaaac gtcggcaatt gacctttatc cagcgccagc
     5401 tgccattggt taatcaccga aagcggcggc gttgcaagcg cataaggttt actgctatat
     5461 agccagcgag tgcctttgac cggaatattt gcaatgaaat ggagatagcc catcatcgca
     5521 tcagagagca ccacgtcgcg tgccatcccg ttaacaccag gatcggtcag taactctacc
     5581 catttattaa actgcggctg gaaaccggca atcgccacct ctgccagctg ttgctggaag
     5641 gctttaacag catcacggtt ttcccacatg ggttgcatat cgcgtgcggc atacaacaat
     5701 tgaagctggt taagataaac cggcttataa cctgcgggta attgagattc gatttgcgtg
     5761 cgggcttttt cagccgcacc ttcaggcaat ggctggatcc ccgccattat tgccgtggct
     5821 tgcgcctgcg gcagtgcctc gccctgttca ctgacagcca ccgggctgtc gccagggatt
     5881 acttcaggct catcggcctg cgcattgaac agtggagcga atgttacggc caggcacaaa
     5941 ctgattgccg acagccgacg accacacatc atattaagca acatcccttg ccccctgttt
     6001 ttatcatact ctgaatagcc aggctgttat tttacgactt gcctgtacaa cgccaatact
     6061 cacgaaagta tgacgagcaa gcctgttact catcaggctt atcttcagta tataaacgcc
     6121 gatacgtttt tacataacct aatgtaaaga agtttaaaga aaagcgcgcc gtaatgtgcc
     6181 acttttttag tgcagaaacg gagttttcgg aaaaagaaaa ggcggcattg ctgccgcctt
     6241 aattttaact cgcctgagaa ggcgcttcgt cacttcctgg aagcgtttca gggagttgag
     6301 gcgcaaatcc tcgcaggccg acgacgtgaa cgtgttcggt attctggaag actttacgta
     6361 ccagtttata ggtggtgcct ttctccgggc tgatattttc cggcgctgcg ataatgagct
     6421 gcatttgcaa acgttcacac aattcaaaca gcgtggcgat ggaacgggcg tccagtcgcg
     6481 ctgcttcatc gaggaacagc aggcggcaag gagagatatc tttaccgcgc aggcggcgag
     6541 attcatcttc ccagctttgt accaccatca ccagaatcga cataccggta ccaatcgcct
     6601 caccggtcga caatgcacca gactctgcac gcaaccagcc atcggaacca cggttaacct
     6661 caacttccat ttccagatag ttgcggtaat ccagcagttc ttcaccgatg gtctgcggcg
     6721 tgcgctgccc catatcaatc tgcgggttaa gacgttgata cagtttcgcc agcgcttctg
     6781 agaaggtcag gcggttgctg ttaaacagat cctgatgctg ctcgtgctgt tcagagagca
     6841 catccagcag catggcgtgc gtttcacgta cgttcacgtt cagacgtacg ctgttcacct
     6901 ggccaaacga tacgttctgc aacccctggt tgagcatacg gatacggttc tgctcgcgct
     6961 gaatggtttt gcgaatgatg ttcgccacgc tgcgggaact gatcgccagt ttctgttcac
     7021 gagaggttaa ttcttcggtc agacggctaa gctcgatctc catctgttcg atagcttcca
     7081 ccggatcatc ggtacgaata atatcctgac gaatacgttc acgcagatgc tgataaaccg
     7141 ccacgaagaa ctgaatttta cgttccggac gttttggatc ttccgacatc cgcagcacgt
     7201 cgcgcagatg ttcgttatcc gccaccgcca gacgcagcgc acctaacgcc ttatccgaca
     7261 tggagcgcaa atcatcagcg gagagataag ccagctcacg acggtgtaag cgacgctcaa
     7321 cgccattatc tttcaccatg cgcatcaccg cacaccagcc cgctttggcg gtcactacct
     7381 gctcgcgcat ctcaaagtaa tcccgctcca gcttgcgcag tttacgggtc aggttgtcca
     7441 tctccgcttc gcagaaggta agcgcttttt ccagttgatt gcgtcgtgaa cggttattgc
     7501 tcagttgcgc gtgcaactcg tcacggcgaa tacgtgcccg ctcttctgcc ccgctatcag
     7561 cacgcacgcc gatatcctgc aattcacgtt gcagatcgtt aagtagctct tttttggtgt
     7621 catacgaact tttcagcgaa gccagcacct ggttgtactg attcaactgc gctgcgtgtc
     7681 cgcgcaacgc ttcacgagcg cgggtacgct ccgcttccgc ctgttccaga cgttcacgca
     7741 gtttttcgtt gagatcgctg ttaccgctaa gcatttctgc cgagtcagag tagctaaagt
     7801 gcgcacgacg ctgcaccact tccgtcaggg caaacgcctg ctgacgggca tcgcgctgca
     7861 tctgctgaga gtacgcgtaa tcttctttta attgttcgaa ctgttccggg tcgctctgca
     7921 ataccgaaac gatcggctcc agtttcgcca gttgattgcc aaactgctga acaaaacgcg
     7981 cggcttcctg ggcttcatcc agacgttcgc ggatttcatc gacgcgatcc gccaggctgt
     8041 catcagccaa caggttgaga cgcggcagaa tgcggttcag cgccgtaacg ccctctttcg
     8101 cctgctcaaa ctgaatacgc tgctgctggt tatcattttc atgattactc aacgcccgtt
     8161 ccagttcgac gcgacggctg ttcagttgac ggatttctgc ttccgggtca gactcaaacg
     8221 ccaccgccag atgactgccg ataaagcggc tgaacgcctg atgcagacgc tgggttttct
     8281 gtacatcaaa ggaaagcgtg gcgaagcgtt cggaaagcac ttcccgctcg gcatggaggc
     8341 tttcaatacg gctttcacgc gcagcacgac caaacagcgg cacttccggg aaacgtgaat
     8401 aacgccactg acgatcggcg attttcacca ccaccgcttt ttccagctca tcaacgctga
     8461 acacgctgtc atcgaatgac tgcggatccc cttcgatcaa atagagatct tccgggcaat
     8521 cggtcaagcc ttccaggtgt tcggttacct gtgacagatc tggcaccacg atggcgtggc
     8581 gtgacgggcc atacagtgct gagaagtacg gcgcatcttc caggctaacg tcgtcataaa
     8641 tttctgacag cagcacacca ccaaaacgct ccgccagcgc gttcagacgc tgatcttcag
     8701 aaccacctgg ctggcttaaa cgttcgatct cttcatcgac ggcgtttttg cgcgcgccta
     8761 cttcatcgcg ttcaacaatt gcctcgcgct cacgctccag caactgttgc agaaattcgg
     8821 tgacatcctg gctggaggtg aactcttcgc cgcactgttc gctcaactgg ttgagactgt
     8881 tttgcgctgc cagccaaacc ggcgcacgct gcatcaaact ctgaatgcga gactgcagct
     8941 gttcctgctc ctggcgcagt gccatccgct cttctcgggc gttagagacg ctatcggaaa
     9001 gagaggcaat gcgtgcttcc agctcctgat gcagagcttc cagttcatca atatcaaaat
     9061 tcttgccctg acgtttgcag aaatctgcca gcagacgctc agcttcttgc tgctcacgca
     9121 gacgctgttc cagttcgctt aagcgcatcc gcaacggctg aacctgctct gccaggtgac
     9181 gctgatcgac cccttcgcgc aatagttcgc gagcgacatc ccacgcctcg ttacgcgcca
     9241 gcgggccgtt gattgccacc accagctgat aagcctgctc aaactggctg tgcgcggttt
     9301 gcgccatgct cattttctgc tcaagagaga gcattttttc ggtcgcttcc agctctttcg
     9361 cctggaaggt ttccagccat tcggcagcgc tgtcggcggt taagtccggc agatggcaca
     9421 gttctttggc acgattaagc gcagcaatcg cctggttata ctggatcgcg cgcgtttgct
     9481 ggacgtccag cgcctgctgg tagtcagcaa gctggctttt cagctcgtcc acttccagtt
     9541 cggcagcttc cgcacgagcc tcgttctctt cctggcgttc gatggcttct gccaccactt
     9601 cgttttgctc ttccagacgg atctgcagct catcgagatc cgcttcgtag cgttcgatct
     9661 tctcctgctg acgcagtgcg gtttgcacca ggttcaggtg atcgctggcc gcctgataat
     9721 ccgcttccag atcgccttcg gcaccgttgt gctcagccag ctctcgcgcc atgtcgacgt
     9781 gtttgtactg ctcagccgcc agttgctgac gcgaagtatg tagctcgcga cgaaactcca
     9841 gggccttatc gagatgaaca cggcgctcgt tggcgtgacg catgtagtcc gccgccacgt
     9901 agttggtggc ttcgctgatc agatgcttaa acaggtcacg gtccgactgg gtgacacgaa
     9961 tcgcttccag cgtcatgcgg ttttcacgca gcgccgcttc catgtcctgg aacgctttcc
    10021 acaccccact gttttccggc aacaggtagt cgcgcagaga acgagtaatg gcgctggaga
    10081 tcccgccata cagcgaggct tcgatcagac gatagaattt actacggtca gacgctgagc
    10141 gcagacgacg cgcgatgatg cccagatcga acatcagcga gtggtaatcg gtaatggagt
    10201 tgaactgttt aaactgcacg ccttccatcg cctcgagctt gtctttcagc tcgttaagcg
    10261 gcagcacgcg cgcctggcgt tcgttcagag tttcggtcac cagttgtgtc ggctgcaccg
    10321 acatcggcag tccctgaatg gcaaacggct tgatatccac tttacgatcg cgtccggcaa
    10381 cctgttgcag acgcacaccg accaccacgc gctggtgacg cgagttaatg gtgtcgagca
    10441 tcgaataaca gacacccgct ttcagcttac cgtgcagacc tttatcgcgc gaaccgctgg
    10501 tggccccggc ttccgtagtg ttacggaaat gcagcagggt cagatcgggg ataagcgccg
    10561 taacgaacgc cgccatggtg gtggatttac ccgccccgtt accgccagaa agcgtcgtga
    10621 ccagctcgtc aaggtcaaaa gttcgggcaa aaaagccgtt ccagttaatc agcgtcagtg
    10681 agcgaaattt accgcgttca atcattattc ttcctctccg ctatctggct gactctcttc
    10741 ggtttcatcg ttgagttgca gatgattttc aatcggcatt gcttcaccat cacgaatcag
    10801 gcgacgctgt gcttcacggg gatcgtcgcc agcacgcaca tcggcgccga agcggaacac
    10861 cgattcggta atgcggaact tgctgctgtc gtggcccata aaccacacca tgcctaaacg
    10921 acgcagacgg ttgagcgaag aacgtacttt ttcctgcaac ttctgacggt caacgtctga
    10981 accggttgaa cggttgttca ccagtttcag cagtttcgct tcatcggcca gggtgagcag
    11041 ttcgtcgtac agttcctgtt gagtaaaaat cccctcattc gccagccgtt ccgggctgag
    11101 atagagataa cagaggattt tcccgaccat catatccagt tccgacaaga cggaacgagg
    11161 gatcagcgtg gtggaacgtg ggcgtagata gaagaaccct tccggtgcac gaataagctc
    11221 aacgttataa cgcgcgtaaa actcttccag atattcctga aaatccatca ggaatgcatg
    11281 attatccagt tcgtcgaggc caatatggcg gcctgaacgt aaggcgctat ccagcgccgg
    11341 aaataacgga ttcgccagcg cctgcgccag cttaaccggc atcacttgtt caatatttgt
    11401 cgatgacatg cgcctgtacc ttggctccgt aatcattaat cggctgccat ttcgctggca
    11461 gtccggtgaa atctgcttgc gctacgccaa gacgtaccgc ctgatcaata acaatgcgcg
    11521 caacgtcaaa gtgacgtgca cgcggatact gtgacagata ttcgcgtacc accagaccaa
    11581 gatccaacgg cacttgtctg gttttgtaca cggcaagttg ttcttcgatg atcgccgcca
    11641 gctgttcgcg gatctcgtta aactcttcgt actccagatc ctcaggaagt tccccagtca
    11701 cttcttcatc gcgcagtacc atctcttcgt cacgcatatc cagcagacga tcggcattgg
    11761 cataagttag cgcccacggc tcatcaaaat aggtttgtac cgactgacgt aaccgctgag
    11821 caaagacgcg gtttttatcc atatcgatcg cagtacgaat aaatttgtgt acgtggcggt
    11881 cgtagccaat ccacaagtcg atggattgct ggccccaact gataatacgg tcgagtttgc
    11941 tctgaagatc gaacaccaga cgatcgacaa aatgcaggtc gtcatgggtc atcgtcgcat
    12001 cctgaatgcg taacaggttg gcctgcaatt tatcgcctgc cgcttccagc gtatcctgca
    12061 attcacgcag cgttccggaa gtttctgaaa gcaacaattc acagctggaa atcgccgccc
    12121 gccagtcttt gttcaacaac tgggcgatat catccttcac ctgctgctgc tgttcatcca
    12181 tcagacgttg cgtcaggtcg atactgtcga aaatttctgc taccgaatat ttcagtggcg
    12241 cataaacatt gcggtgccag tgaaattcat caccgccctc ttcggcggca tctgctgcgc
    12301 gtttgagctc acccgccaca atcgacaatt gcatagaaag gcgcagcgta gaaaactcgc
    12361 gctgacgaat gtaatagtca gtgatgccga tgccgagcgg cgtcagacgg taaattgcgt
    12421 tcccttccgc ctgctcgctg gtaaagcggt tcagcagacg ttgacgcacc atgtcgttga
    12481 tcgcgttgtt ggcgcgcacg ccgatggttt cgctggtttg ctcaaacgca tcactcacat
    12541 ggcggaatgc atccaccagc tcgccttcac tcatctcacc atccagacgc tcgccgttca
    12601 gcgtggcaac cgccagcaga aaagagagtc ggtctaccgg cagcgagatg gagaagtcat
    12661 tttttctggc ccaggcaacc agttcgggga ctgtctggga aaattcactc atactttatc
    12721 cttgctctgc ggtttgcgcg cggtgacatg aatataacgc cccagggtaa tatacggttc
    12781 ctgacggcaa taacgcgttt ctaattcaag taatgcttca tagcagtcgc gctgctggtg
    12841 cttctcacgc agataatcat gaaacacgcg aacgcctgtc ttacccagaa tttgccaacc
    12901 agcttcttcc agccacagat aaacctgcgc cgggtcgcgt ggataatctg gcgaaagcgt
    12961 ccgttttttc tttttcggca ttcccgcctg cacgtaatca aaattcccgg cgaccatgtt
    13021 atgcatcaac aaaccatgcg cattgtagaa cattaacgac aacacgccgc ctggacgcaa
    13081 cactgaccac agggtctgta atacgctgcg gggatcggcc acccactcga gcaccgcatg
    13141 gaacaatatc agatcaacgg gcgtttccaa atgcgaagca acatcctgag cggcgcaatg
    13201 tataaattgc atgttgtcgc tcacaccttt tgcttctgcc gcctgttttg cgcggtcgat
    13261 catctgcgca gaaagatcgc ataaaatgac ctgatgcccg cgctcggcca ttttgattgc
    13321 ggtctgccct tctccaccgc cagcatccag cacacgcagt ttttgcgggc ctatttctgc
    13381 cagcacgcga tcgagatcct gccacagaat agcctgtcga agctgccctt tggtggtgcc
    13441 gtaaatgtta cgggaaaact tttccgcaat atcatcaaaa ttgcgatcct gcattgcttt
    13501 ctccaccggt tggctaagac gttttctgat aaagacgcta ttttgtcaca gccacggcga
    13561 gaatgaaccc gtctggtgta atatcgctgg cgatcccttg ctatatggtt aaaaaaggaa
    13621 ccagaaagga tgctttttac actgaaaaaa gtgattggca atatgttgct gccacttccg
    13681 ctaatgttgt tgattattgg cgctggcctg gcgctgctgt ggtttagccg ttttcaaaaa
    13741 accgggaaga tttttattag cattgggtgg ctggcgctct tgttattgag tttgcaaccg
    13801 gtagccgacc gcctgctgcg tcccatcgaa agcacctatc cgacatggaa taattcgcaa
    13861 aaagtggact atatcgtggt gctcggaggt ggttatacct ggaacccgca gtgggcgccg
    13921 agctctaatt taatcaataa cagtctgcca cgcctgaatg aaggtattcg cttatggcgc
    13981 gaaaatccgg gatcaaaact gatcttcacg ggaggcgtag ctaaaaccaa tacggtgagc
    14041 acagccgaag tcggtgccag agttgcgcaa tcgctgggcg tgccgcgcga gcaaattatc
    14101 accctggatt tgccaaaaga taccgaagaa gaagctgcag cagtgaaaca ggcgattggt
    14161 gacgcacctt tcctgctggt gacctccgca tcacatctgc cgcgggcgat gatctttttt
    14221 cagcaggaag ggttaaatcc gctaccagcc ccggcgaatc agttggccat cgactcgccg
    14281 cttaatccgt gggaacgggc aatcccttct ccggtttggt tgatgcatag cgaccgcgtt
    14341 ggctatgaaa cgcttggccg gatctggcag tggctgaaag gttcgtcagg cgagccacgg
    14401 caagagtgat tttgatgcca gatcgaagtt acgccgatta aatcgtccgg tgttgaccag
    14461 ttgcgcaact tcatcccata acacatacaa ccaacgccgc cagatgaacg actccgccac
    14521 tggcgcgcgt tgcagataac tccagagcaa atcttccgcc agagaattat ccattagtcg
    14581 gaacagttcg tattctcgcg gtgcccaaag cattagtccc ggtccgacca tcgccagtaa
    14641 ctgatcgctg cgcgaatctt tcagcatgct gcgtaaacag aagttaccgt gaatcagtac
    14701 acaattgtcg ttaaagcctt caaataatgc cgggagacat tcgcgagtgc gaaacaggat
    14761 ccgcttatcc tgcatcgata gaccggtgtt attgaactga ttgagcgtgg tccatagcac
    14821 ttcaacatgt tgccggtacc atgagggcca gaaattttcc tgagtattgt cgaccgcgcc
    14881 gacgcaacca cgactgtcct gacggtgcca ggccagtaag gcttcaacga tttggtcttt
    14941 gagttgttcc cagcgttctg gtgttcgggc tggtgcctcc accgaaacac cacgcattcg
    15001 ctccagtagc aggacatcgg ggccggggtg ttcttcatgc gtcatcacac cataaatagt
    15061 cggcatacgg acagtcccac ttcttgccag catggtggtt ttccatgcca gttgtcgggc
    15121 ttttccgggc gtactaaagc ttcttgccat taacggcatt gggtttccct ggctgtcata
    15181 caaagcccac aacgccgtat ccgctttttc attgacgcac tcaatacgac tgagtttttc
    15241 gcccagtaaa tggcttaatt cggcacgcag ctgttccatt cagattaccc tcataaaagc
    15301 tactgctttc ataatgagcg ccaatataaa agatgtcacc tgaatgagat caattgttga
    15361 aaagaaaaat tgaggaaaat cgtaacggta cgacactcct cccaaaattg gctggagtgt
    15421 cgtgaagtga aattagcgca tttcagcgcg aacgcgctcg agatcttcag gggtatccac
    15481 acctgtgcca ggaacttcct gggcgacagc aacgtggatt ttttcgccat accacagaac
    15541 acgaagctgc tctaacattt cgatatgttc taacggactt ggctgccagg taacataacg
    15601 acggataaag cctgctcggt agccataaat accaagatga cgcaggaagt tatcgccaac
    15661 ggtttcaagg tcttttgcaa aacgatcacg atcccacgga atggtggcgc gagagaagta
    15721 cagtgcgtac ccttcggcat cgagaaccac tttcaccgca ttcgggttaa acgcttcttc
    15781 cgcattgtgg attggcaccg ccagagtcgc catacccacc tgacgctgag cgaggttatc
    15841 agcaacctga cgaatgattg tcgcagggat catcggttca tcaccctgca cattaacgat
    15901 caccgtgtcg tcgctgaatg cgcatttttc gacaacttcc gccaaacgtt ctgtacctga
    15961 ctgatgatcg gcgcgcgtca tacatacttc gccgccagcg gcttcaacag cgcgggcaac
    16021 atcctcatga tcggttgcca cgatgatgcg ctcggcacct gattcgcgcg cgcgttcaag
    16081 aacatgaaca atcatgggtt tgccgttaat atcaaccaat ggtttaccgg gcagacgcgt
    16141 ggacgcgtaa cgcgcgggaa taatgaccac aaaactcatg atttaccctc atcagcagtc
    16201 agcacgcggg cttccgtttc cagcaacact ggaatgccat cgcgcagcgg gaaagccagg
    16261 ttgtccagtt tgcaaataag ctcttgtttt tcctggttat accaaagttt tccgttgcaa
    16321 accgggcagg caatgatttc aagcagacga tgatccatag ttcctccaga tggatcgggt
    16381 tatgaatgca taaatcttat cataatcatt tatgcgacgg ggtctatttc ccagccggcg
    16441 cggcaatcag taaagaggcc ttgcgagcag cgtcctaatg tcacccgcgt tgcctgctgc
    16501 cagttagcga aatcagtaat cgcctgacgt aacccttttt gcagcgttgt cgttggttta
    16561 atgccttcct gtaaccacag agagataact tcaaggatgc ctttctggcg atgcattttg
    16621 gaatccattc gcccaactaa ttgcccacga tgtaataacg gcagaacaaa atagccatac
    16681 tggcgtttcg gcgctggggt atagcactcc agccggtagc taaaatcaaa aagctgctcc
    16741 gcgcgtttgc gatcccagac aacaggatca aaaggcgaaa gtaccgcgct gtgcgttgca
    16801 gtgagctttc cggcgagcgc acgctcgagt agcggcagca aatcagcatg cagccaaaga
    16861 ttgcccaatt tttcaacatg cacagcaatg atttgctgct gttcagccct cgcttcacgc
    16921 cacgccgcca gtgccggacg ttttagccgg tagtaatctg ccagccactg ttcgcgaaat
    16981 attcccaggc tacgcgcact gttatccagc atgatgattt ctgcttctgc ttgcgaaacg
    17041 agatcgcgct catcatccca gtcaggcatg acacggtggg ttaaatcata aacgcgctgg
    17101 aagttgcgcc gttcaatcac catcaccttt ccggcagtaa ataaaccttc cagatgtcgt
    17161 ttatgcggct tccactccca ccagccgctt gcacctttac gaggatgctc aaaatcggct
    17221 gaacgtaccg gccccctatc atgaatatgc tgaattaaca gtgcaatttc cgccgcatgt
    17281 tcctgcatcc aggcgtcttt atatttccag cccatttttt caggtgccag catgcggtgg
    17341 cgaataagac gaaaatcgct acgcggcata aagcaggctt catgcgccca gtattccatt
    17401 aattcgccac gcgccagaga ctcatccagc cactgggcag gataatttcc cagacgactg
    17461 aaaagcacca gatatgggct acgggcaaca atattgatgg tatcgatttg cagcaaggac
    17521 atgcgggaga tcgttgccgg aatatcctcc aacgacgctc gacggtgggg tttgtttaac
    17581 aggccttgtg cagcgagatg aagattacgc gcatcagcaa gggagaggtg cggcagagac
    17641 attcatgact ccatcaatcg aacgctgccg cggcgtaact agttgccaga agccagcgaa
    17701 gttagttgcg caagcagttt cgctggttca tcacctgaaa gctgtgcgtc tacaggcaaa
    17761 taccaccaat tttcttctgc aaaggcccgg catttcaccg catctttttc agtcattacc
    17821 agcgtttgtc cggcgcttac caacgcgctg acatccgcat gattcaaaaa ctgatgatcg
    17881 gccagcggta cacatttttc gggttgtacg ccacacatct tcagcgtggc aaaaaagcgc
    17941 ggcggatgcc caatccccgc aatcgccacc acatgttcaa gctgagcaac gtcacaacgt
    18001 gtaccggtac gtaaattcac cgcctgaccc ggcagcagat gcatggggat ttcaccgctg
    18061 cgggggacac cgccgttgac gattaccgca tcaaccaact ttaagcgccc tgctcgctca
    18121 cgcattggcc ccgccggcaa ccaccagcca ttgccaaagc gacgcacacc atcaataacg
    18181 acaatttcca catcacgcgc cagacgataa tgctgtaaac catcgtcagt tacgatgatc
    18241 tgcacatcag ggtgttgcgc cagaatggct tttaccgcgt cagaacgcac gggagaaacc
    18301 gcaacaggcg cgccagtgcg ttgataaatc aacacaggct catcacccgc ctgtgctgtg
    18361 gtggtatctg ccgacaataa cagcggataa gattcagcct taccaccata tccccgcgat
    18421 acgaccccca cgcgaatacc gcgctgttgc agctgttcca ccagccagac aacgaccggg
    18481 gtttttccgt tgccgcctgc agtgagattt ccaaccacga caaccggtac gggggcacgc
    18541 caggcgcgct tcagttttag tttatagcaa agacggatcg cgccactcac caggccatac
    18601 aaccaggaga gtggcagcaa tagccgccat aaaggggact caccagacca gattttttcg
    18661 atcattggcc aaactgcatt ttgtgaagtt gcgcgtaaac gccacggtgc tcaagcaaat
    18721 cgttatgcgt accgcgttcc acaatgacac catcctcgac aaccacgatt tcgtctgcct
    18781 tttcaatggt agacaagcgg tgggcaatca ccagagaggt acggtttttc tgcaactcat
    18841 ccagtgctgc ctgaatcgca cgttcggatt cggtatccaa cgccgaggta gcttcgtcca
    18901 gaatcagtat cgggctatca cgcaacaagg ctcgagcgat agcaatacgc tgacgctgac
    18961 cgccagagag caacacgccg ttttcaccaa tgactgtatc gagaccgtta tccatcttat
    19021 tgatgaagtc catggcgtag gccatacgcg cagcttcttc aatttgctca cggctgtact
    19081 gttcagtcct tgcgtaagca atgttgttgg caaccgtatc gttaaacaga tggacattct
    19141 gcgacaccag agcaacctgg ttacgtaacg acgccagggt atactcgcgc agatcgtgac
    19201 catccatcag gatttcgcct tcatcaatat cgtaaaaacg tgtgattagg ctggcgatgg
    19261 ttgatttacc tgaaccagag cggccaacca gagcaaccgt cttccctgcc ggaattttca
    19321 ggttgatgtt acgcaatgca ggcacatcac gtcccggata agtaaaggtg acattgcgaa
    19381 actccacgtc gccagtcgca cgctcgatca cgcgcttacc ttcgtctttc tcctgctcac
    19441 tgtccagaat ggtaaacagc gtctgacaag ccgccatacc gcgctggaac tgggcgttaa
    19501 cgttggtcag cgatttcagc ggacgcataa gtgcaatcat tgaagagaaa acaacggtaa
    19561 tcgtaccggc agtcaggcta tccatgacac ttgggaagct tgccgcatac agaacaaacg
    19621 ccagcgccaa agaggcgatc agctgaatga tcggatcaga gatggaagag gctgaaacca
    19681 ttttcatccc ctgaagacgc attcggttgc tgactttatc gaagcgtttc gtttccactt
    19741 cctgaccacc gaaaatcaat acttctttat ggcctttcag catttgttcg gcgctggtgg
    19801 tcacctgccc catggtgttc tgcatgtttt tactgatgtt gcgaaaacgc ttcgatacaa
    19861 cgcgaatcgc aatcgaaaca atcggtgcca gcacaatcaa aatgatcgac agttgccaac
    19921 tgtaatagaa catcatgatg aacaggccga tgatcgacgc accttcacgc acaacagtaa
    19981 tcagtgcgcc ggaagaagaa gaagcgacct gttcggaatc gtaggtgata cgtgacaaca
    20041 gcgtacccgt tgactgtttg tcaaagaatg aaaccggcat tcccatcatg tgaccaaaca
    20101 ggcggcgacg catagtcatt accacctttc ctgataccca ggagatacag tagctggaga
    20161 tatagctggt gataccacgt aaaatcatca gcccgatcac caccagcggc atccacatca
    20221 gcacggagcg atctgtttta ccaaagccat catcaagaag tggcttaagg agcgataaca
    20281 tgaaggtatc gctggctgcg ttgaggatta acgctacgcc cgccacgatc agacccgctt
    20341 taaaaggcgc aatggttggc cacagtcggc ggaacgtctg ccacgtagag agatctttgt
    20401 cgttatgcat tcaaaaaacc agcatttgtt gaaatagccg catattctac ccgttatcca
    20461 ctggcacgcc aaaccactga tgataccaac gaggtaaaat ttgctctctg aggctgctaa
    20521 tccaccagcc ttgcgctgaa aaattgaccg ttatttgccc ctgatgtgga gtatcaagcc
    20581 acttataacc ctgttgatga tagcgatatt taactttgct tgagggcagt cgccatgcgt
    20641 tatagcgcga tgccgatgcg agtgccactt ttccattcac tcgctgaatt aatggcaacg
    20701 atgatgaggt attactgcca tggtgaggta cctgaagtaa tgttgcctgc atttgctgcc
    20761 agtaacggct tagcatcttt tgttcagcgg gggcttcaat atcaccggtt agaagaatgc
    20821 tattcgtccc gtcatcaatc ttaaccacac aggaatggtt atttccttta tcgttgctac
    20881 cttgtaaagg ccagtgcgcg ctgaaacgca atccttgcca ttgccacgct tcgccacgca
    20941 cacagggctg atgatgttcc cagtttaacg gacttcggat ccataacatc ggccatgtgt
    21001 gcaatattga gtccagccct ccccggtgat ccagatgttc atgactcaaa ataacgcctt
    21061 ccggttcaag attatgccag tggagccagg ggataatcag ttgttgccca ctatcccctt
    21121 cgggccaggc cagtcccgtg tcatagagaa tcgctttgcc gtttcgcgca atgaccattg
    21181 ccagcccctg cccgacatca agcatgtaca cttgccactc gtcaggtcga ggtttttgcc
    21241 acagcggcaa gcacatcagc aagcctacag ccacacacat tgctggcaac gttcgccagg
    21301 cgtttaatcg ccataccacc agtaagaacc atggggaaaa tgttagccat tgccaacgtt
    21361 cagcaatgtt gatccaccct tccggcaaac tctttaaccc ccagaaaagt aaagccaaag
    21421 accggtcggc aagaaaccat aacccttgtt ccagaataaa cggcccgctt aaatgcacaa
    21481 ccatcgcggc gagaatcaac ggaaccgtga taaatgtcac caggggaatt gcaaaaagat
    21541 ttgcaataaa cgaggtcaga ctaatgccat gaaatatgac aatttgcacg ggcataagca
    21601 ggagggtgat tcccagttgc agatggatga gggaaacaac cgcgcgcaat accggcggca
    21661 gctgccactc cggacaggga aaccactgat accaaaaaat cagtgccgcg accgcagcgg
    21721 cagaaagcca taaactttgc gagagaatgg caacaggatc catcagcaaa attgccgcca
    21781 gacaacatat ccatacatcc cagccactcc attgtcgtcc acttaactta agcattcccc
    21841 aaatggcaag cgccaccacg gtacgcaatg caggaggttg cattcccgta agccaggcat
    21901 aaaaagcagc acagcagatt ccaccaatta atggcatttg ccagtggagc cagcacccag
    21961 gcagaaaaac ttgcccaccg cgaatgagtc ctgcagccag caacgccgca aaagcgatgt
    22021 gcaatcctga tatcgccatt aaatgcgccg ttccagtatc acgcatgata tttttgattt
    22081 ctttggggac ggataaccgt tcccccatac ctaatccaag aataaccgcg ttccacatat
    22141 agggttgcag cgttgtttgt agtgacgcca gatactgtgc acgcaggcta caattcggtt
    22201 caatgacact tgcctgcaga aaacggccgg tgagcggttg atgctgggca atggcataac
    22261 gctggctatc aaagccgcca tcattaagtt gaccatgaac tgcacgaact ttgagtttca
    22321 ttgaccatac ttgtccggca caaaccgctt gcggaagata ttcgccatac agtacgaggc
    22381 ctggcgcagg gaatatacgt cgaccttgta gatgagtaat ttgaccgtaa tgagtggtca
    22441 tgccgtcagt agcaatgatc tcaacaattg catcctgcgt cgcgccagtt aaggtttctc
    22501 ctgcccacaa aatttgcttt gctgctaata tgccccaaac gaaaaacagc aacgtcagcg
    22561 cgatataacg gacggtttta acagggataa atgccagtac acaggcgaag agagtcagaa
    22621 acgcaagggt tactgtccct ggcaattggg gcaaaatcag caacggaaaa attccgcaaa
    22681 ttatgcatac gccgactgtc gttattttca tgcaccctcc ctgtttatag aaagtgtgca
    22741 actttgttga acggttgtct ggtgggagtt ttgcgttccc cgaggcacgt tcagaagaat
    22801 ttaacgatag ggtgaaaact gctcaaaact ctgcgaggca gcttccagtt ccagattaga
    22861 gaaatgaaaa aagcacccga caggtgcttt tctcgcgttc aagtttgagt aaaaacttaa
    22921 ccgtaaatat tggcgcgatc gcgcagttct ttacctggtt taaagtgagg aacgtatttt
    22981 ccttccagtt ctactttatc gccagtcttc ggattacgtc cggtacgtgg tgcgcggtag
    23041 tgcaaagaga aactgccgaa accgcggatt tcaatacgct cgccctgcgc aagagtcgag
    23101 gccatatgct ccagcatctc ttttactgca tcttcaaccg tcttggcggg aatgtgcgat
    23161 tgctgggtgg caagtctttc tatcaattct gacttggtca tgattcctcc ggttccttaa
    23221 aggcaaatta agcggctgct gttgctacat tgattaaggg cggccgtagc cgcccttagt
    23281 gcttgattac aggacgaaac ctgcaatctg tcaagtaaac tcaacaaact tcggaataaa
    23341 aatcccgaag agtcagagaa ttactcgcct ttagctgctt tgaaagcttc agccattgcg
    23401 ttgttggaga agtttgcatc ttcctgtttg ttaacagttg cgattgcatc tttctcgtca
    23461 gcttcgtctt tcgcacgaac agacaggctg attgcgcggt ttttacgatc aacgccggtg
    23521 aatttagctt caacttcgtc gccaacgctc agaaccaggg tagcgtcttc aacgcggtca
    23581 cgggatgctt cagaagcacg caggtaacct tcaacgccgt cagccagttc tacggttgcg
    23641 cctttagcgt caactgcagt tactttaccg gttacgatag cgcctttctt gttcagagca
    23701 acccagttgt tgaacggatc ttctgcgagc tgtttaacgc ccagggagat acgttcacgt
    23761 tctgcgtcaa cctgcagaac aactgcagcg atttcgtcgc cttttttgta ttcacgaact
    23821 gcttcttcgc ctgcaacgtt ccaggagatg tcagacaggt gaaccaggcc gtcgatgccg
    23881 ccgtccaggc cgatgaagat accgaagtca gtgatagact tgattttacc ttcaacacgg
    23941 tcgcccttgt tgtgggtttc cgcgaactgc tgccacgggt tagctttgca ctgtttcaga
    24001 cccagggaga tacgacgacg ttcttcgtcg atatccagaa ccataacttc cactacatcg
    24061 ccaacgttaa caactttgga cgggtggatg tttttgttgg tccaatccat ttcggaaacg
    24121 tgtaccaggc cttcaacgcc ttcttcgatt tcaacgaagc agccgtagtc ggtcaggttg
    24181 gtcacgcgac cagtcagttt ggtaccttcc ggataacgtt tagcgatagc tacccacgga
    24241 tcttcgccca gctgtttcag gcccagggat acacgggtac gttcgcggtc gaacttcagc
    24301 actttaacag tgatttcgtc gcccacgttg acgatttcgc tcggatgctt aacgcgtttc
    24361 caggccatgt cagtgatgtg cagcaggccg tcaacgccgc ccagatcaac gaatgcaccg
    24421 tagtcagtga ggttcttaac gataccttta acttccatgc cttcctgcag gttttccagc
    24481 agctgatcgc gctctgcgct gttttcggat tcgataacgg cacgacgaga aacaacaacg
    24541 ttgttgcgct tctgatccag cttgattact ttgaattcaa gctctttgcc ttccaggtgc
    24601 agagtgtcac gcaccggacg aacatctacc agagaacctg gcaggaacgc acgaataccg
    24661 ttcagctcaa cagtgaagcc gcccttaact ttgccgttga taacaccggt aacagtttca
    24721 gcatcttcgt aagctttttc cagcgtgatc caggcttcgt gacgtttagc tttctcacgg
    24781 gacagcagag tttcaccgaa gccgtcttct actgcgtcca gagcaacgtc aacttcgtca
    24841 cctacctgga tttccagctc gccctgggcg tttttgaact gctcagccgg gatggcggac
    24901 tcagatttca gaccagcgtc aaccagtact acgtctttgt cgatagcaac aacaacgcca
    24961 cgaacgatag aacccgggcg ggtttcgatt tcttttaagg actcttcaaa gagttgagca
    25021 aaagattcag tcatgtttaa tcttcaggtt tatatttaac gtccacctgg ctccatgccg
    25081 gatggggttg tttaacatac ccgctgtcat tccattgcaa cgggggtact gcaaattcgg
    25141 tcgcttatgc gagagccaat ttctggcgcg cgtattgtag cgctttttca atcacttgct
    25201 caatgcttaa ggtggtggaa tccaacacta aagcatcggc tgccggaacc agtggcgcta
    25261 ccgctcgatt acgatcgcgg tcgtcacgtt ctttgatctc ggccaaaagg cgctcaaagt
    25321 taacactaaa gcccttctcc tgcaactgta gcatgcggcg atgcgcacgt tcttccgagg
    25381 aggcgtcaag gaaaattttc actggcgcat cagggaatac caccgttccc atgtcgcgcc
    25441 catcggcaat caggcctggt aattcgcgaa acgcgcgttg gcgacgcaat aatgcttcac
    25501 gaacgcgtgg gaatgccgcg acttgtgaag cagcattcgc cacctcctga gtacgaattt
    25561 cgccgctgac atcttcccct tcgaggatca cttccagatt gccgttggtt gacacaaaac
    25621 gtacatccag atgggatgcc agcggcacca gcgcatcttc cgatgcaaca tcaacatggt
    25681 gatgtaatgc cgctagtgcc agtacgcgat aaattgcacc cgaatccagc agatgccatt
    25741 gcaacgcttc cgccatagcc ttacacaagg tgcctttccc tgcaccgctt gggccatcaa
    25801 tggtaataac cggggcaatt gccgtcatct ttatctcctt aaaacaggcg taccgttaac
    25861 ataaccgcgc gcattatacg cgccgacgcc acaaatcgtt aacgttgtgt gcaaaaacca
    25921 gttttgtctg tattcagatg gggatttatt gcgcaattat agcgggctgg cagaagacca
    25981 gcccgaaaga tgacaatgat ttacttacca gaggcgatac gatcacggat gtgttgcgca
    26041 cgctctgttg atggtgggtg agaatcaaac atggactggg tgtgaccgcc atccaggcta
    26101 gcgagttttt caaagctacc aaccagcccc tgggtactga tgccacgttt cttcaacaga
    26161 tcaaaggaga aatcatctgc ttctgactct ttatcacggg agtatttagc attgatagcg
    26221 ccttctgcga tatcacccaa ttgtgagcgg gaaagctggg aagccacacc gctggtagct
    26281 gaaatggcat cgcgtgcggc cacgatcgca taagaagctt tcatttcagc cagcgagtga
    26341 cccaacgcga cgtggcccag ttcatggccc agaacgcctt caatttcgtt gtcgttcatc
    26401 atgtccatca ggccactgta gacacgaaca caaccgttcg ccatcgccca tgcgttgacg
    26461 tcgctggtca tataaacctt atagttgact ggcgtgccat taatattgtt acccaatgct
    26521 ttggcgattt ttgccagacg tttcccgtac ttgctggatt tgcttgccac tttattgccg
    26581 ctgtccattt cgacacagcc ctgattggcg attgctttag catcagcatc gcttaatgtt
    26641 gcggctttat aagcattcag acctgagctg atagccaaat cggtattaat accgtgcgta
    26701 ttctgacatc ctgtcagtaa ggctgcagag gtcgcaatcg ccagcaataa tttagtattc
    26761 ttctgattag acttgagata aatgacaggg aataagtgag atatttatgg gaagaactgg
    26821 gtcaaaatgg gaagagacta ggaggaatat gcaataacct tacagtatgt gaaaatcaga
    26881 ccggcccgaa aaaaaaacag aactcgcgcc agcctgattg cagatattac tcttttttct
    26941 ccgtatctgc atcgggttca tcagcaaata gcttgccctg catccgatcc agttcttctt
    27001 ttcttacccg cttaaccacg ctgtagaccc actgaagcga aacaccaaat ttgcgggcca
    27061 gttcgtggtg gttgcgtccg ttaaactccc tgaagatttc ccggtcgcgc tgactgacct
    27121 tccataccat gcccatcggg aaataaacgt tttgcccgcc ccagacctgc atcattcggt
    27181 tcgcgacggc ctgaccaatc tggtcggcaa cttcgggctc aatatcaata atctcgcgaa
    27241 cggtctcaga ggtatgctgt gccagttcca ccaggagttc cggcccctta cttcgaaact
    27301 gattcaggtc gctcatgttt gactcccgca gctctgcgct gccacttctt cagtttctca
    27361 ataacactgc ttgcctgttc agtactgagc cagcgcaggg cgctgatgcc cgtttcccgc
    27421 ttgatccacc tcgctaatgc attttctgaa cggtcacgaa caatgccggc agcagccatt
    27481 tcaagccata gcgcacgaat tttcctggac tgcggatggt tatcaagcgg taaaccggag
    27541 ctggcttttc tggctggttt aacgcgaaag cctttctttt tcatggattc cagcacgcag
    27601 tttagttgtg tggtatccat tcctttggtt gaggctttac cggtcagccc ctgtaacatc
    27661 tggcggtagg tgtcttcatc catacccagt tcattacggg caatatggat aagctgtatc
    27721 aggcgttttt tctgcatatc atctcctttt tttcagtctg tcggcaacaa tgtcggttgc
    27781 agctggcagg gtgactggcc agaacaacat gacggcggtc atgtacagga tgtaatgcgc
    27841 ggtgtcgtag tgcctgccat agcccaggga acgatgaagt cttgcgctgc atgatcctgc
    27901 gtacatatac cagaaaagaa aacagacaat ggtttcagta gtcattctga atacctcccc
    27961 attcgatatg aatattacgg gcagcaatga cagggtcgtt attccaccat gcacctgaca
    28021 tgtatttttc aacctgttcg cgtccggcaa taacaccaat tgtgatcccc ggcctgacgt
    28081 tcttaaaaaa ggcgcgggca aaaaggtatc tggcagatat tcggcaggct tttaatttcc
    28141 ggcttttacc tgatagcgta atcatctggc ctccagtttc tgttgttcct gcccactgac
    28201 cgggcggtgc agtctgacgt tctgcccttc acgaaacccc gcatagcgcg aggcgtcgcc
    28261 attgcggctt cttcccggtt tacgcgccct ggtggtttgc gtctgcgggt atttatgttc
    28321 cagccactgc tgcatcagtt cacgctcatc atcggtcagg gcaaaggact gtatttcact
    28381 gataacggcc agcacccagc cttcggcaaa ctggtctcca cggctggtgc gggtggcggt
    28441 ttttattctt ttgttctgtg cactgatata ctgctgacgc gcctttttca gctgacgggc
    28501 cagcacttcc caggtgtatg cagccagtgc cgcacgttcc cggttgccgt agaaccccac
    28561 agacggatgc gtgccggggt gaatgatgga gttaacacca aatacctcgc ggatgatgtt
    28621 catcaggccc agcatgtagc gcggtggacg gagactgcct gtcggccagt agtgactgat
    28681 ggtttcatca atatcactca tagcaatgtc ggaatgtgtg atgccgtgaa catccatcag
    28741 tttacgggct cggcgcagtg ccagagcggc ctcgtgcggg ttgccggatg cggccagcgc
    28801 cagcaacttt ttcagtttct caatgtgttt attctggtct gtcattgttc tgtatctccg
    28861 gtatttttct gccgtttcca tgcccggaca gcgtcggaca gctctttcag gctgtatgct
    28921 gctttaagct tttcccataa ccactgtgtt gttatgtgca tcagcagggc agccagggct
    28981 gcccccgcac tgaggcatgt cgccaggcca gtaagaatca gtgtccatgc ggtgatttcc
    29041 ctgagtatgt cagccattga atgcctcccg gttactcgtt cgtgtaaatc acgcccagtc
    29101 gtgcagccag acgttccagt tttttctgtt tgtggaagtc aatcagccgg tccatcccct
    29161 gaaggcgcag ttgctctgtc atgatttcca cgtctgccag ctctgccgcg aggtcacttt
    29221 cgctgccctg tccgttcagg ttgcgggcgg cactggccgc cagttcagcg gcctcttctg
    29281 ccagtttcag ggcctgtgcg tccggcccga aacgctgcag ggccagacgg tagagggcgg
    29341 tgcgggtgag ttcggtgttt cgtgtcatgc cgcgccctca gtgcttccgg ctgacggtga
    29401 tgtgcaggcc gccttctgca gtggtttcca tccggtacgg cacctcatgc tctgccgtgt
    29461 gggtgagtgt gttcatcagt acctgcaggg cagcctcctt tccgttggtc gccacaatgg
    29521 cctgtgtggc catgctgatt agcgccgtca ggacgtgctt cacatcggtg aggtcgcggc
    29581 attcacactc gttgacatag tgttcaacaa gggttcgggt gcgctgtcgt gcttcctgtg
    29641 gggtaatcat tgcgagtcct ccctgtcagg gcgggagaat tccatgacgg gcacgtctgc
    29701 cgaaaaatgc tggctgcagt acgggcagac cagggtgacg cgtaccgcag ggatgtggta
    29761 tttaccggac atcacggcga tggcgctgtg aaaacgcagg gctgttatat ccctctcgca
    29821 ctgaatacat ttaaatatca tgatttaatt ctcctctgtt tccggcgtgc agaagcccgc
    29881 ggcgctgacg tcggaataaa aaagaaaatg tttttattaa ataattaacg tggtgtgttt
    29941 actgcacatc ctgctcaaaa ggaattattg aaaaatcctc aatgtcgctt ttaatggaaa
    30001 taccgggaat atttttcacg gcctcttttt cattcaggat agcgtcttta tttatttcct
    30061 cttttacacg aataaagcgc tcaagcccca gacgtctcag taattcaata acattatccg
    30121 ccccacggat actgactgat ggcggacggt ttcgccactg cacctcgccg gtggtgaggt
    30181 tagcgaactt caccttcccg ttgccggtca gttcatcacg gtgtgcctca caccatgtct
    30241 gaataccgga ctgcagttcg gccatgcgtt ttttcaggct ctcggtgagc ggggcataac
    30301 gtgcggtgat atcgccaatg gcgtcattca tttctgtttc agccctgacc agttcacgtt
    30361 gtgcgtcacc gagtagtctg attccctcaa tgacctcttc acgtgtcccc ggcacccaga
    30421 gtgctgctgc ggccttgata cgttttgcac cttttgtact ttttgccatt ttttatgatt
    30481 tctccagttg tgctgattac cacaaagatt ccggccacac gacgcgacag ccgtgcagtt
    30541 cgaaaacgcc ctgacggaaa tatcccctgt ggtcatgtcc ggtatacaga taacaggcct
    30601 ttccctgctc aagcatgcgc atgcaatgcg cactccggga aacgcggatg acaggtttgt
    30661 tacccctgat ggtgatgctt tgtacatccg tgttcgtcgc cttaagcgcc ataatggctg
    30721 actgcacttt gctgatttgc tggttgatac ctgtggtgga tttcattatt aaaccccttt
    30781 gacaacgtca gcgttgacct gtggaacccc gatttcagcg gccagattca tggcggctat
    30841 taccaggtta ctgacggcca gcggatacag caggctgacc atatttttac ggtgacttcc
    30901 cggattgctc aggcgggcac gtatggcatc cactgcaccg gcgtccataa tgtccgtcag
    30961 ctgtttaccg gcccgttgca gtttgaacgt cagaaattct tcaaggttat tgtccagagg
    31021 cagaagttcg accacctcac agcgctgaac gacttcacgg acttccatat tgcgttcaga
    31081 cagttttgtc gccagttccg gctggccaat cagcacgatg gacagcagtt ttttgaaacc
    31141 ggactccagc tcaaaaaagc gtttgaggtg tttcagtgtc ggaatgggca gactgtgggc
    31201 ctcctcaatc accagaacgt ggctgaaccc cgcctggctg ctgtctttca ggacgcgatg
    31261 caactggcga aaacgggcgt cctgactgcg tctgatgctt tccagtggtg cgatggtact
    31321 gataatggct tcggcaatag ctgctgcctt cagggttttc cctttcacat cgttgtcttc
    31381 catagcgatg atgtatggct cgataacaat taccggcgca ttctcgcggt tgatacgttc
    31441 agtcaggtcg cggcgcagcg tggatttacc cgcaccggac tcaccgatga cggccataaa
    31501 cccaccatga cgggctgtct ggtacagcgc ctcacgcacg tagcgaatgt ccggggtggt
    31561 gaacacatca tcagaaccct gcatggcttc gtcggcgaac gggtcacgga aaagaccaaa
    31621 cgcttttttg gttgctggaa ataacacctg ttttttgagt aacatattct cttcctcact
    31681 gaggctcgtt ttatctgtgg tacccgctgt acggggcgtg gccgcgccct gtacagcatc
    31741 aaaactcttc gttgtatcaa tcccctgact ttccagccag gacgcaagac gccggcgcac
    31801 ttctccgggg ctggtgcggg gccacgcgtt atgattcaca atctgggcca gcgtggcctc
    31861 agaaacatcg acagctctcg ccaccaccgc ctgtggaata cgggcctctt tcagttgctg
    31921 cttcagtacc agcatgtttc cctcctcagt tgccgttaac aatgctgata acactgctgc
    31981 gggccggtgt ggtcagcgtg gccatgactt catccagtgc ggcttccggt acgccgtccg
    32041 ggtactgtgc cgttaactgg cggtaatgtt ccggcgtcca ggtaaggccg tcggcgctga
    32101 acttatcgcg cagggctttc gcggcctcca catgagtcat gggacgttgt tcagtgcgcg
    32161 gcccgcgtac gtcagaggcc tgaccacgct tcggcatata ggccggaagt gtggtgtcgt
    32221 cgatatgttt atacgggtca agccgcccgc cgaacggcag cgccttcgcc ttgcgtgcgg
    32281 cggctgcatc tgcggcgttg tctgtaccgg taaccagcgc ttcggtttct tttgccgcca
    32341 tctgtgccgg ggtttccggc agggctttgt aactttcgcc aaataccgcc gcgccttcag
    32401 caaagccaaa ctcgttcttt ctgacctctt cgaccaggaa gaacgtctcg tggccgtcct
    32461 caccggtcag aaccacctgt gccacatcgc tgcgccatgg gttacgggta atcatcagtt
    32521 tttcaccaac cagtacgccc ggtaccgttg atacgtcaaa ttcagtgccc cggaacgaga
    32581 cacgaagttt tggcgtgact ttgcggagtt ctggtgccgc cacagccagt tcacgacata
    32641 cctcaacgga aggcgctttt ttcagctgct cagcagtaat cttcagccag atatccgtgc
    32701 gggttttacc gtggcggctg tgaacagccg tggcgttaaa gtggctgcgc catttcgcgg
    32761 ccagcgcgtt gagttcttcc agactgtgaa ccggccggaa cttcagaccc ggctccagct
    32821 tgcgttcgat aatgtcacgc gccttttcca cctgtccggt ggcgcgggcg ttatgcggct
    32881 tgtgcgctat caggtcgatg cccagtgagc ggcacatgtt tttcgtcata cccgcggtgt
    32941 ttgccgagcc ggggtcgaga tagagtattt tcggcacgcc gtgcagcacg tctgcgccgc
    33001 cacgctcctg catggcgttg ataagcacag aacacaggtt ctcacctgat tccgcgcccg
    33061 tcacatactc aacgtaaatc cagccgctgg catggtcggt aatctcgtaa ctccacacgc
    33121 ggtcactggc gatacgggca aggttagcgg gcttgttctt gtagaacttc gcgctgtcca
    33181 tcacctgcag ccctttatgt ccattgctca ggtagtaaag cgtgcagagt gaggcgtcaa
    33241 tctgccagac atgattggga tgcagactgg ccacttcggt atgtggtgca ggggcatcca
    33301 gttgttccgg gtgcaggcca tagttacgca gagcacggct gatggcatct tcagacaacg
    33361 ggaaaaactc actggtggtt tcatctgttc tgcctgcggt gataaagccg ttagcccgca
    33421 gggtttccac tgcatctgcg atggaataga gacgcttacc gttcttgcgg gtggcctcac
    33481 gcagtgtggc agatatcagc gcggcttcgt cgcgggtcag ggcgctgcgc ccggcatcgg
    33541 cgcgtttttt gcgtttatca gtcacagata cctccttcag cctgcgcagc agagtggcgc
    33601 gggacatgcc aagttcagca caggcagcgt cgtatattgc accgcgttta ccatgccccg
    33661 cgtcacgtgc cgcgcgggct gtttcaacca gtttttcagt cagtgccggg gtcatagctt
    33721 gtcttccctg ttatccgatt ctggcggcaa tttttctggc gaggtcgcac agtgtttcgc
    33781 catcgcaggt tttcaccacg gtatttaccg gttgcggtgc aactaccggg cgtaatgctg
    33841 actgattctg cagacgtctt atctgctgca tcagcatcac ctccatcgtc agtacaaacg
    33901 ggtgcgtctc ctgtgcgggt acgcagattt caccggaagg ggtcaccacg cggaaaccct
    33961 gcaccatctc cggcagcaga ttacacagtg cgtgccccag tgcctccggg ctgtccatca
    34021 gggtattttg tgtgttattc gtattcatta ctgcatatcc tccggtcggt tgatttcagg
    34081 ttccggttcg gtcagccagg cgggggcatc gttacccgat ggagcggcct caaggtcaaa
    34141 cgtctcacgt aatgcatgaa cactgcgttc aatctggcag accagaccgg ccatgaaatc
    34201 tctgggggta tcgatcatgt ttttggtgca gtattcacac agggtctcaa aaacactgga
    34261 cagccggacg gcgatagccg attcggcctc aaccgctaat gccgtcactt ccgcccgcaa
    34321 ttttttcagt tcttcgtctg gcggcggagg ttgcaggcgc gatttcttct ccagttttgt
    34381 ggccagttcg ttgatttttt cgtcctttcc ggcaagcacc tgttgctgag ctgcgttggt
    34441 ttcgcgcgct tcacgcaggg cggccttcag ttcgcggctg gtcatgcgat caatatcatc
    34501 aagcgttgca ccggcgacgg ttccaccatc agctaatgcg tcgagttctt cgtcatccag
    34561 aaccatcagt tcatataact tggtttttcc caaaacgctc agcgctgagc gttttggctg
    34621 ttcatctcca ttgccgagaa atttcacgct ggcctgcatc atgcgacgag ctacctgtgg
    34681 tgctaatccg aggtcgtttt ccagaatatt tgtgaaatca ccatgcggtt cattttcttt
    34741 taagatgatc agccgcttac ccgcttccaa catagattca gcactctgcg ccatataaaa
    34801 acgtgcttcg tgaacgatgc gttcacgttc ataaggcagt ccatcaccaa actgctgcat
    34861 gatctccatg cgatgctgtg tcatggcatt caggctgacg ttgagatcat ccgacagcgg
    34921 tacatcggcg ttcagttcag tgttaatcgg tgatttggta cgtcccattg attactcctt
    34981 acaaacgact acctgaaatg acacgttggt taatttcgtt aatacgatcc tgtgcacgtg
    35041 ccatctcgtt actgtgggcc atggcgatct gcagtaactg gatgcccggt gcaaaacgtc
    35101 cgttctccag tttcagagcc agcccttctt caatgagggt gttgagtgcc cggttgatgt
    35161 ttgccggtga ctcgtgcagt gctgtcgcca gttcaccgtt agaaataccg ttcagggcat
    35221 gaccgcgcag cgctttgagt acccggagga tgcgactgcc ggagcttgaa acattcgtct
    35281 tactcatctg catacctctt ttgcgatatg tgataacctt ttacaaagac tcaaaatatt
    35341 taggccgcct gagctgttga tttcagtccc aacttcacgg cgatttcatg ggatttaccg
    35401 taacgggctt tggtctgccc gttgagaacg cggtatacct cattgcggtt gtagccgttt
    35461 tcttctgccc agcgggtgaa agtgatcccg cgctggcgga agagagcttt gacttgttct
    35521 gcagtcatcg ttgtctcctt tgttgatgca atgatgtttg ccttatatgt gttagattat
    35581 tgctcataat tatgagcata tcaagataga gattcacttt tatgagcaaa gatataggag
    35641 agcgcgttat ttctgtgagg aagaaagcag ggttgaacca gagagaattt gcagctcgtt
    35701 taggtatttc taatggtggt atcagtcaga ttgaaaatgg aaaggcgatg cctggagggg
    35761 agtttctgtt aaagatgcat caagagttta atgttgatgt aaattggttg ctcactggaa
    35821 tgagcacaga tggtatgact agaaaccaga atcaaatttc cccagaaaaa caaaaactaa
    35881 tagatacttt tgacgacatg acaccagacc aacaacgctc atttcttgag attgggcgat
    35941 tctatactca gccaaaacca ggaaaaatcg caggctgata ctgacccgga cacaaaggac
    36001 aggtggttga tctggatgct ttcagaaaac atcgccagtg acatagtgga agttgtttgg
    36061 tttaccggaa agcattgtgc tttccggttt tgtttcagaa gttgcagatt agcagttcac
    36121 ggcgcggcgt gggtttaccc gccagactgt agttgatatt cactgtctgt atgttcagtc
    36181 cagtgaacac ctcgcgcatc tgcgggatgt cgttcaccga aattatcatt tttcctctga
    36241 tgcaccgggc cagctctgcc agccgcgagt agttcccttc aggaaaatcc acaccatagc
    36301 cttccgtacc caggtacggt gggtcgcagt agaacagcgt gtgcggacga tcataacgct
    36361 caatgcactg ttgccagtcc atgtgttcta tgactgttct ggaaagccgc aggtgtgccg
    36421 ctgacagttc ttcttcaata cgtagcaggt tgaagcgtgg cggggatgtg gtggaggtac
    36481 cgaagctgtg ctctgccacc ttgccgccaa acgcctgctt ctgaaggtag tagaaccgtg
    36541 ccgcacgctg gatatccgtc agtgtttctt ccggggtgat ctgcagccat ttgtagatct
    36601 gacggctgac cagcgcccat ttgaactggc ggacaaattc ttccaggtga tgctttacca
    36661 cccggtacag attcaccagt tcaccgttga tatcattgat gacttcgatc ttgccgggtg
    36721 tcttaagaaa atagagcgct gctgccccac aaaacggctc cacataacat tcatgcgccg
    36781 gaaacagcgg cagaatgtgt tttgcaaggc ggcgtttgcc accaatccac ggcacgatgg
    36841 gtaatgtctg cattttcata atctgcaagc cttttacaat tattaaaaaa tatggcagtc
    36901 tggtcaggtc tcgcgagact gactgaacct tggtcggctc acagcgcatt cctgtggggc
    36961 gatgaccagc ccggctttcg ggtgccgggc tggtcgttct ttcatgatgc ggcagggatg
    37021 gcgggatttc tgttaatgtg gtttagggat tatccggggc gattttctga ccttatccgg
    37081 tccagggcat aagtaatacc ttctgcaaaa acagaagtat caaactgctt ttgtattccc
    37141 tgaaaatgaa aagcggcacg acagacactc agttctccgc ggtataaccg gataatatct
    37201 tccagaacat tgttcactgc ttcagcacgg cgctgatgtt ctgttaacgt taatcgggga
    37261 ttcatgatgc actccttaaa tgatgatttt tatgtactca ccggcaaaat attctgccga
    37321 ttactgaaca cgcatccggc acctcaggtt ttgatttatt ctggtttttt cgatatcccg
    37381 gaccaggttg atcacgacca gaaaaagtgc cggtgtttta ttgatacgct gatatggctt
    37441 cgggataaca gctatattcg ttactctcag ttgctggctg tgggagcggc tgatgtcgtc
    37501 ctcacggaaa aaacgctctg tctgctcaat gccatgccga cctttctgca gccccggccc
    37561 gtaccattta aagagtcact tctggatgca gtaaaaaccg gtgaaaatgc agtcatcgct
    37621 gaatgcgtat cccggattat gcgttacgtg tttaatgaac gtaagtgagg tttatccgct
    37681 gtctgcataa tcccgatagc cagtgcgagg ggaagtaatt ccggggaaac tctttcaaac
    37741 agcgatgccc attctcctct cgccatcact ctgtccagcc cgccctccag ttgcatttca
    37801 gcaatcttcc tgatttcccg gaacaacgcc gctttgatct cttcatgctc agctttattt
    37861 tccggtgtca gaaggtattc gtcgaagaac cggggaggct ggttaatcgg gatgtgttcc
    37921 tgtttgccgt acttcgggga cgaacaacat tctgttgact gaccagtgag tttacgtaac
    37981 tccttcatga gcagctcctg atcgtaaact gtcatcagtg ccgcaatggt ggcatgcagt
    38041 gcatcgggca gtatccaggg ttgccggccc gtggggcata taacatccag cagctcattc
    38101 gtccgacaga tacgctcctc gccgtatgac aacaggccct gtcccgtgta ttcctccagc
    38161 ctgatcactg tgccagcccg tggtttctgc catgtgctca gatcatcccc actcatacgg
    38221 cgctcaccgt ccacgtctgc ggcgtcgaca atgacgctgt ttccgaattc ctgtttcacc
    38281 acttcctcta ttcgcgccag tacgtaactt ttaccgctgc ccacaggacc ggacacggtg
    38341 acagtgatga ccggattcag tgtctgcgtc atatcgtatt ctcctctgtg aataattcat
    38401 cgtttatcct gcacatcccg tatccccggc tctcttaacg cgctttaaaa tccttcgcgc
    38461 cctgtatttg tgatgctgtc tccaccagat aaggagacac acatgaaaaa cctgaaaaaa
    38521 ttcattcccc ctgttaaaaa acctcgcctc agcggctggc tgctgacctc agtgctgttg
    38581 ctgggcacca tcgctctggt ctcaccacag cagctgcctg ttgtgatcta caagctggca
    38641 ctcatcacgc tggcagcagt gctgggttac tggcttgacc gttcgctctt ccccaaagcc
    38701 cgtcccggtc agtacctgaa acatgacgac aggctgatgg ctgaagggcg tttcccggta
    38761 cagaccggcc ttcacctggt gttttctgct gcgttaatcc gccgtgcact gattgttgca
    38821 gcggtctgcc tggctgttgc gatggggctt taatcatgaa ctggccgcag atcacctgga
    38881 ttgtatgcat gtccctgaaa ctggctttcg aagcctttcg ggtcttcatc agaaccgaac
    38941 gcctgtcagt ccgcgccggg acctttctgg ttcatatggc atgggtattc tttgtcgcaa
    39001 tgttgctctg gtgtggcggc tttttcagcc aggcatgcgc agcacaacct ccgcaggctg
    39061 cgctgcagta tcgcgatgat gtgatccgta atgcccggct tgaatgggga ctgtctgcgc
    39121 cggtggccga tttcgccgcg caactgcatc aggaaagcgg ctggcgacct gatgcgatct
    39181 cgccggctgg cgctcaggga ctggcgcagt tcatgcctgc cactgccgac tggataagcc
    39241 agttgatacc gatgctgagc agtcgtgagc cgtttaatcc ggcatgggct atccgggcgc
    39301 tggtcagcta tgaccgctgg ctgtggcagc gtgtcagcgc cgccagtgac tgcgagcgta
    39361 tggccatgac actgtcgggc tataacggtg gtctgggctg ggtacaacgg gacaggcggc
    39421 ttgcatcaca gaaaggtctg gacagcaccc gctggttcgg acatgtcgcc acggtgaatg
    39481 ccggacgcaa tgcggccagc tggcgggaga accgccatta tccgcagcgc atcctgcgcg
    39541 aactggcacc gcgatatctc acatggggag gcagcagttg tgtggcatct ggttaaaaag
    39601 ctgccgtggc gcggcattct gctggccatt cttatcaatg cctttctggt cggcctgtat
    39661 gccatgggat acagaagtgg tcatgactct gcaaagcgtg acggtgatac cgcgctaagt
    39721 cagttgcagt cagcatttga cgcgtacaaa acggagcagg caacgcttga gaatgctgcg
    39781 ctgcgggcct gggccagacg gtatcaggag caggtggccg ccgggcagcg ggctgaagcc
    39841 ggttatcttg agcagattgc tcaactggag agccggaaca aacaactaca ggggcaaatt
    39901 aacgatgtca cacagcgctg gattgatgaa aaaggtaaga gtcatcccat tgagtgcgtg
    39961 tttactcgcg gtttcgtgcg ccagtacaac gccgcactcg gatatgacaa cgcatccgtc
    40021 gacaccggtc attcagactc agttgccgcc actggcaccc gctctggcac agcgaccggg
    40081 caacctgaaa ccactgacac ccggttacgc gattcgggtg tctcccagcg tgacgttctc
    40141 gccaacatca tcgacaacgc atggcaatgt cgtcgctggc ggaaccagat aaacgcgctg
    40201 ctggatgaac gggaaggatt acagaaatga cactgcaggt tgaattctgg acggtggtga
    40261 gttttctgct caccttcatg aggtttgtgg gagggctcgc caaatggttg ttcagtaaaa
    40321 cagaagaacg ccaggcggca cgattcgcct cccttgaaca ggccctgcaa caatccgcct
    40381 ccaactgggg cgagctggaa aaagaattta tgcgatttaa agcggattta ccgctgaatt
    40441 atgtccgtcg agaggattat atccgtggcc agacagtcat tgaggccaaa ctggacgcgc
    40501 tttataacaa actggaagtg gtacagcagt accgcaatac cggaggtcaa taatggtcga
    40561 tattacccgg gtacgccgcg aatccctgcg ctggagtctg ctggttgccc tgaacaagac
    40621 ccgcccttac accgccagcg agacgctgct gctggatgtg tcccgtgcca tctacccgga
    40681 caccacaccg ctggaactgc gccgcgaact ggattatctg gctgaccgta aaatggttga
    40741 gctggagaaa aaaccttctg gcgactggtt tgctgacctg acccgcctcg gcgtagacct
    40801 ggtggaatac accgtggaat gtggtccggg tattgcccgc ccggaaaagt actggagtga
    40861 ataatggcca gacgcagcac aatagaaaag ctgccggaag acgtgcgccg ctggcttgaa
    40921 cgggcgctga ctgaatccgg cttcagcggg tataacgagc tggagtcact gttgcgtgag
    40981 cgggggtacg tcatcagcaa atccgctatc catcgctatg gacagaagat tgagcgccgc
    41041 tatggtgcta tccgtgcggc aacagaagcg gcccgcatgc tgaccgaagg cgcagcagac
    41101 gatcaggatg cgcgttcgga ggctgtgata gcccttattc agaccgagct gttcgagagt
    41161 attgtccagt tgcaggaggc ggaagaaggc gaagtcgatc ctaaagaacg cgtggccctg
    41221 ctgtcgaagg tggcgaagaa tgtggctacg ctgtcccgcg cgtccgtcaa cctcaaaaag
    41281 ttccagtctg aagtacgggc cagagcgcag caggcagcca gcaacgccga gaaaattgcc
    41341 cgtaaggggg gactgtcaag cgacgcagta caggcgcttc gtcgcgagat tctggggatt
    41401 gccacatgac aaaatcatcc ggagtgattt taaacgccgc tggcggcggc cccgaaggga
    41461 tgagtcccat ggacgggagg aataaccttg ctcccgtttt gcctgatacc tcggcgctgg
    41521 atgcccctcc cgttctgttg ccttaccagc agcgctgggt ggcagacacc tctccgctta
    41581 aggtgataga aaagagccgt cgtaccggta ttacatgggc tgaggcatcc gataacgtac
    41641 tgaccgccgc ctcttctgcg ccagcaggcg ggatgaatgt gtattacatc gcttataacc
    41701 aggacatgac cgtcgaatac attcaggcgt gtgcgatgtg ggcacgggca ttcaactatg
    41761 cggccagtga aattgaagaa ggattctggg aagaggacga cgacgacaaa cacatcaaga
    41821 cttacaccat caaatttcct gactccggct ttcgtattgt tgcgctctcc agccgcccgt
    41881 ctaacctgcg tggccgtcag ggtattattg ttatcgacga agcggcgttc catgagcaac
    41941 tggacgaact gctgaaagcg gcgctggcga tgcttatctg ggggggaaag gtacgcgtta
    42001 tctccaccca tgacggtgac gacaatccgt tcaatacgct tatcggggat atccgtgccg
    42061 gacgtcaggg aggcagcata catcgcatca ctttccggga agccgtatct gaggggctgt
    42121 tccggcgcgt ctgtctgcgc accgggaagg aatggtcgga ggcatccgag caggcctgga
    42181 tggcatcggt gtacaaattc tacggtgccg gcgcatccga agagcttgac tgtattccgg
    42241 ccaacggtgg cggtgcctgg ctgtcccgtg ccctgataga gtcccgcatg tccgctgata
    42301 cgccggtatt gcgtctgacc tgcaaggaag gttatgaact gctgtctgat gaggttcgct
    42361 tccgcgagac gcaggactgg cttgatgagt atctgaaacc attgctggag gcactcccca
    42421 ctgatgcccg ctctttcctg gggcgcgact ttggccgtag cggtgatttg tcggtggact
    42481 atcccctgct gcaggagaag aacctggtac gacgcgtgcc attcgtactg gagctgcgta
    42541 acgtgccgtt cagacagcag gagcaaatca cctggtatct gatggatggc ctgcccggtc
    42601 tgctgggtgc agcgtttgat gcccgtggta atggtgccta tctggctgaa tacgccatgc
    42661 agcgctacgg ctccggccgg gttcagcagg tgatgccaac cgaaggctgg taccgggagc
    42721 atatgcctcc ggtcaaagct gcactggaag acggcaacct ggtggactta ccaaaggatg
    42781 aagacacact ggatgacctg cgggccgttc aggtggtgaa cggtgtcccc cgcgtaccgg
    42841 agcaacgctc aaaagcaaag gctgacggtg gtaaacgcca cggggattca gccatcgcac
    42901 tggcgctggc gtatttcgcc agccgtgaaa ttaacaaagg gccggtgaag gcaagctcac
    42961 gccgtcgtcg tcaggcggcc cgtatgctgg agggattctg atggcgaggg gtatctgggt
    43021 ttcacccgat gaatttgttg ctttttctga gcctcagaaa tcactgaccg cgcagattgc
    43081 ctcccgcagc cgcgcgatcg acttttacgg actgggcatg tatctgccca atcctgatcc
    43141 cattctcaag gctcagggac gggatatccg tatctaccgc gaactgcgca ccgacccgct
    43201 ggtcgggggc tgtatccgca gacgtaaagc agcgctcaaa tcgctggagc gtggactgga
    43261 gcgtggtcac gcctctgccc gggtcttccg tttcatccgc gacatgctcg acgatctgga
    43321 tctgtcccgc atcatcggtg aaatgagcga tgccgtgctc tacgggtatc agccctgtga
    43381 aatcatgtgg ggccgttcgg tcaggtcgtg ggcggtgacg gatattgtcg gcaaaccgcc
    43441 tgagtggttt cagtttgata cggacaactg cctgcgcttt cgggcgcgtg atgcgggtgt
    43501 ggagggtgag ctgctgtcac cgtcaaaatt cgtggtgccg gcacaggatg cctcgtatga
    43561 caatccttac ggtttcccgg acctgtccat gtgcttctgg ccggtcgcct tcaagaaagg
    43621 cgggatgaaa ttctggctcc gctttgccga aaagtttggc tccccgtggg tgatcggtaa
    43681 gcacccgagg ggcgcaaatg atgcagagat tgaaaaactg ctggactcca tggagcagat
    43741 ggtggaggac gcggtggccg ccatccccga tgacagcagt attgagctca gggcagcgga
    43801 tggcaaggca gacagcagcg aggtatttcg cgagctgatc acactgtcac gcagtgagat
    43861 ctccattgca ttactcggtc agaatcagac cacggaagcg aacagtaaca aggcctctgc
    43921 acaggccggg ctggaggtaa cggctgatat ccgcgatgcg gatgcggaca tcattcaggc
    43981 ggcagtgaat caggtcatca gaacggtggt caccctgaac ttcggcgatg tgccgtgtcc
    44041 ggtctgggcc atgtgggaac aggaggccat tgatgacacc cgtgccaccc gcgacgaaaa
    44101 actcacccgg gcgggtctgc gtctgacccc gcagtacttc aagcgtgagt accaactgca
    44161 ggacggcgat attgacgaga caccaccgtc ggaacgccag aataacatgc tgccgctgtc
    44221 atttgccgag gcgattgatg ctgatattca ggctcagcag cagcttgacg acgcgctgga
    44281 cattctgatg aacggaggcg cgttaaatgg cacgctggaa cccgtcctgg cacctctgtt
    44341 caggcgggtc gaaaacgggg ttaacccgtc tgagctgctg ggcgaactgg cggagctcta
    44401 ccctcagatg aacacggacg atctgcagga gcggctggcc cgcattctct ttgtggcaaa
    44461 tatccgggga cgtctgcatg agcgtgacaa cggctgaact ggcgtactgc atgacgcttc
    44521 ccccgaagcg ggcaatcaac tacctgaagt ccaaaggata tcagattacc tgggactggg
    44581 aagaaatgtg gcaggaggcc catgcccgcg cctttaccgt tgctaaagtg acccgcctgg
    44641 atatcctgga agatattcgc ggggcactgc agcaggctgt cgatgaagga aaaaccgatc
    44701 gctggttccg gcaggagctg gagccggtgc tgaagcgtaa gggatggtgg ggaccacgtg
    44761 acacgactga cccggtaacg ggtgagccgg tcaccattca gcagggcagt ccgtggcggc
    44821 tcgataccat ctttcgcacc aatatgtccg tactctacag cgccggtcgt tgggcggaac
    44881 agatggaaaa cgtcgacgac aggccgtact ggatgtatac cggcatcaac gacagccata
    44941 cccgcaggag ccatctggcg ctgcatggtc tggtgctgcg ctgggatgac ccgttctggc
    45001 aggcatttta cccgccgaac ggctggcgct gccgctgtag tgttattgcc ctgagtgcgg
    45061 cggatgtacg tgcccgtggc ctgaaggtta tcagctccgg ctctgccatg ggccaggaac
    45121 tgaaactggt ctcagagaaa accggcgaaa tgcggaacgt ggccaccttt aataccggca
    45181 ccacgaaggt gaccaccgac gtcggctggt cttatgctcc gggggcagca taccgtcccg
    45241 acctggcccg ctatcagggt acgcttcagc cactggcaca acaggaactg agaggataac
    45301 aatggcttcc gataacctgg tcagtatcac cattaacgat aagtccctgc gccggagcct
    45361 ccgtgcgctg gatcttgctg ccacagacct ggagcccgcg atgcgcaaaa tcgccggaac
    45421 cctgctggcg gaaacacagt ttaactttct tgatgagggg cgtccggggt ggatgccctc
    45481 gctggcagcg gaagaacgtg acgggcagac actgcaggat accgggcgtc tgatggggtc
    45541 agtatcaacc gaccatgacg accggcaggc tgttgtgggg accaacgttg tttacggtgc
    45601 cattcaccag ttcgggggta aaacggggcg taatgagtct gttgaacttc cggcccgccc
    45661 gttcctgccg gtgacggggg atggagaact acagcctgaa gtggtaatcc ccatcctcga
    45721 taccattgtc cgccatcttg aatcagcggc ccgtcgctga gttttctctc ttcaggcggg
    45781 tgatttatca ttgccagcga atgaggggct gtattacctt tataaaggct ttacagcctc
    45841 tgttttataa ccgcctccgg ttcaccgcat tgctttccct gtccttctcc cctgatgttt
    45901 tctaaagcag attaaaatcg ccgggcctgc atttctcaca aactgtctcc gacaacataa
    45961 cgcgggacag caaaatgtca gccattcaca tttttaaagc cggtactcat accgatatgc
    46021 acggcaaaaa actgccgttc acgccagacg atcttgccgc ctgcgtgaaa gcctatgacc
    46081 cgtccgtcca tgaagcacca ctcgtgattg gtcatcccag aacggaagac ccggcgtggg
    46141 gctgggtgaa agccctgtcg ctcagcggcg tcgatctgat ggcagagcct gcccagctgg
    46201 acccgcagtt tgctgagatg gtcaccgacg gacgattcaa aaaagtgtcc gcctctttct
    46261 acctcccgga ttcaccgtcc aatccgaagc ccggcgtgct ctaccttcgc catgtgggct
    46321 ttctcggggc acagccacct tccgtcaagg ggctgaaaca ggtgtccttc agtgagcagg
    46381 aagaaggtgt ggtggagttc gccgactggc aggccatcac gaatgcctcc ctgtggggaa
    46441 agctgcgcga ttttctgatc gcccgcttca gtctggacga ggcagaaaaa gtcctgccgg
    46501 aatggcagct caacagtctg cgcgaagagg cgtaccgcga cacactgtcg caggatgcag
    46561 caggtgcaca attcagtgag acaggcccgg ggccgtcttc cgcaagtaac gaggaatcat
    46621 cgatgacaaa agaagagatt gaagcccttc aggaggagaa ccgccgcctg aagcagcagg
    46681 ctgctgatcg cgatgcgcgt gatgcacagg tcagacagga gcaactgcat aaggacaatg
    46741 tggcctttgc agaaaaactg gtcgcagagg gccgtctggc tccccgcgcc tcctccgtgg
    46801 tggttgccct gctggatgcc gtcgccggtg gcgacaagcc ggtggagttt gctgaggggg
    46861 aaagccgcac accgctggcc accgcctttc gttcattgct ctccgacggg gagccggtga
    46921 tgaatttcgc cgaacaggcc acaaaagagc gtgtcggcga cacggtgaag gtggatgtgg
    46981 cagagtttgc ggaagccgat cctgagcgtc tggccctgca tcagaaagca gtggccctgt
    47041 ccaaaaaaga aggcatcagc tatgaggctg ctgtcgcacg ctgcctgtaa tttaaggaga
    47101 gagcatgtct gattacttaa aaggtaaacg tgtcgttgat ccggtactga ccagtatcgc
    47161 ccgtggctat aaaaatgccg cattcatcgg cgaacgtatt ttccccgtcg tgctgacgga
    47221 caaggaaggc gtgcgtgtac cgaccttcgg gaaaaccgcc tttgtggaat atgacaccga
    47281 gcgtgccgtc ggggcggaca gcaatgttct ggtccgtgaa aaaacaggca cgctggacct
    47341 ggtgctgggt gaacacgatc tggctgcgcc ggtggactat cgcgagcagg cggagtccat
    47401 gtttaacgaa gagagcaagg ccatccgtcg cgccacgaat ggcgtgaacc tgcgccgtga
    47461 acttatcgct gcccgtctgg ctcaggatga aaaggtctac cgtaccgggc acgtcaaaaa
    47521 actgacagcc agtgatcgct gggccggtgg taagggggac cccatcgggg tgattgaagc
    47581 cggtatggaa gcggtccgta cggccacggg gctgcgtcct aacctgatga ccatgggggc
    47641 cggcgtgatg gcgctgctga agttccaccc ggcgattcag gccgccatcg gagccaacga
    47701 acgcaagcgc atcaccacag aaatcctgca ggacctcttt cagatcgaag agatcgtcat
    47761 cggtgcccca gtctccctgc cgtccatgaa agcggcaatg gataagaaca gcgtgccggc
    47821 ggatatctgg ggagacaatc tgatgctgca ctatgtcggc aaaccgcagc cgggggcgga
    47881 cagcgcggac gagaacgagc cgtccttcgg ctacaccctg cgtcgtaagg ggatgcctgt
    47941 tgccgacaaa tacgacggag ccggtggcaa ggtgaagtac tgccgttata ccgatatcta
    48001 caaagtcgcc gtggttggtg gcgatgccgg gtatctcatt accggtatca gtaaataagg
    48061 aggcgttatg ggaaccactc agcaggtcat tctgatcaca accgtaacgg caggggcagc
    48121 actggcacag cagcgttttg tcggggcaga taataccccc tgtaaagccg gtgccgcagc
    48181 gctcggggtt gccgaagtgg atgctgttac cggcgacagc acgccggtga gcgttctggg
    48241 cattattgct gtcgaggccg gggccgctgt cagccgtggt gtggctgttc agtcagatgc
    48301 tcaggccaga gccgtgccgc agtccggcga cggtaaatcc tgtggtattg cacttgatga
    48361 agccgggggt gaaggcgacg tcattcgtat cctgcgcggg gtgtgacatg tactgcaccc
    48421 tggaggattt gcttgcgcag gtgccggagc ggacgcttat cgagctcacc agtgaagaga
    48481 tggacttcga ctcacctgca acagtgaata cccgtgtggt ggacagctgt atccgctatg
    48541 ccgacgagct gattgatgcc catctgcgcg gacgctatat cctgccactg gcagagatac
    48601 cgactgttct gcgggacatt gccatcacgc tggtccgtta ccggctctac gcccgccgcc
    48661 cggaaggtga cctcccggat acggtgaagg atgaccacaa agaagcgctg cggcaactca
    48721 gggagttacg tgataacagg ctcacgctgg ggctgccgtc cactcagaaa gatgtgcctg
    48781 agcctggcga gtttcgtgta cgcagtcgcc cggccacttt cggcggtcgt gacggtttac
    48841 tggagaaata ctgatgaacg ttctgcccgt ccttgatgcg gtactggccc ggttacgcga
    48901 gaagctgccg caactgcagg tggagtactt cccggagaaa ccggctgaat atcgcctgaa
    48961 ccatccggtt ggcgcgttgc tgttgagcta tgccggttcg cgctttgaca ggccggatga
    49021 taccggtgcg gtgatccagt ctcagactat ccagctctgc gtcacggtgg tcttccgcca
    49081 gctcaacggt aaaaaagggg cgattaatgt cctggatgct gtccgccgca ttctcggtgg
    49141 ccacaccccg cccggctgcc gccgccgtat ctggctgacc cgcgaggtgt ttatcggtga
    49201 agtcaggggg ctgtggcagt acgccctcga cttcgcgact gaaagcgtct ttatcgaaga
    49261 cagcgattta ccgtccggcc cgctgttaac cgaagtgaac tatgaggaaa gcgagtgatg
    49321 aaagaatacc gctattccgg cccggccagc ggcgtcacgc tgtcggacgg aaccgaaatc
    49381 ctgctctggc cggggaagac ggtttccctg ccggaggagc atgactacgt gaaggtactg
    49441 gtggcgctga aacatctgac gccggtacct gaagagacta aacccgccgg cacaccggct
    49501 gtgcagtcac caaagcgcag aagcagcagt gacagcgaag tgaaaacgga ggacacccat
    49561 ggcagctaac tatctgcatg gtcccgaaac cattgaggtg gaaaacggtg cccgcccggt
    49621 taaaacggtg aaatctgccg ttattggcct gattggtacc gccccgatgg gggatgtcaa
    49681 tacgctggta cagtgcctgt ctgagaaaga cgcagcggca tttggcagcc agttcaccgg
    49741 ctttaccatt ccgcaggcgc tggatgcgat ttatgaccat ggtgcaggca ccgttctggt
    49801 cattaacgtc ctcgacccgg cgaaacataa aacggcgatc gaggatgagg tggttacctt
    49861 tgacaaatcg acagggcagg ccagactggc gcatccggtt gtcgctaatg tggtggtgaa
    49921 aaacagtgaa ggcagcacca cccacacggc gaacacggac taccgtgttg atgcgcaggc
    49981 gggtgtgctc acgaacctgg gcaaggctat tgaggccggt ggcagcgtga aggtgagcta
    50041 tgagtacgcg gacccgtcga aggtgactgc ggcggacatc atcggcgggg tgaacagcgc
    50101 cggaaaccga accggcatga agctgcttaa cgacagcttc aacctgtacg gctatttcgc
    50161 caaaattctg attgcgccgg tgttctgcac ccagaagagt gtcgcagttg agcttatcgc
    50221 catggcagag aagctgggcg cggtaaccta cattgatgcg cctgtcggta ccacctttgc
    50281 acaggctctg gcaggtcgtg gcccggaagg caccatcaac ttcaatacca gctccgaccg
    50341 cgtccgtctg tgctatccgc atgtgaaggt atatgacccg gtgacaaaca cagagcgtct
    50401 ggagccgctc agccagcgtg cagcaggtct gcgtgccaga gtcgatctgg acaagggcta
    50461 ctggtggtca tcctccaatc aggagattct ggggatcacc ggcgtggagc gccagctgtc
    50521 cgcgatgatt gatgacccgc agagtgaggt gaacctgctt aacgaacagg ggatcaccac
    50581 ggtattcagc agttacggca gcggccttcg tctgtggggt aaccggacgg cagcatggcc
    50641 aacggtcacc catatgcgta actttgagaa cgttcgccgc accggtgatg tgatcaacga
    50701 gtccattcgt tatttcagcc agcagtacat cgacatgccg attactcagg cgctgattga
    50761 tgcactgacg gagtcggtca acgcctacgg tcgcaaaatg actggtgatg gtgcggtact
    50821 gggcttccgt tgctggtttg atccggcccg caatccggag acggagctgg ccgccgggca
    50881 cctgttgctg agctacaaat atacgccacc accgccgctg gagcgactga cgtttgagac
    50941 tgagatcacc tcggaatacc tgttaaccct gaaagggggc aactgatgtc aaagattgag
    51001 ataaaccgca tcacgaatgc caacatctat ctggatggta ctaacctgct gggacgggct
    51061 gaggaagtta aactccccga tgtctccatg attatgcagg aacacaaggc gctggggatg
    51121 gtgggtaagg tggaactccc ggctggtttt gacaaactgg aaggcgaaat caaatggaac
    51181 agcttttacc gcgatgcgat gctgtctgcc gcgaacccgt acaggtcgct ggcactgcag
    51241 tgtcgttcca gcgtccagcg ctacagctcg caggggctga ttgacgaaat cccgctggtc
    51301 accttcctga cgattatgtt caagaagaac ccgctgggga cgttcaaaca gcacgagaac
    51361 gccgagttct ccagtagctt cacctgcacg tatatcagac aggtactgga tggtgaagag
    51421 ctgctgcaac tggactatct ggccaacatc ttccgggtcg gcggtgttga tcaactgact
    51481 gactaccgta tcaatatcgg gggctgacgg tgagtgttga actgacggat aaaggaggac
    51541 gatgtgcggc actgggcatg tcaaatggta cgtggtttac cctccttgat attccggggg
    51601 tggaaaccct ttttaatacc cgtaaaacca atgacccgat tgactgcaca cgttcaaagg
    51661 cccgcaaact ggcggatttg attgaagcat gggagcctcc cgaccactgg ttctccggca
    51721 tcggcaaatc tgagggaaag acgcttctca tcgctttcct gcgtaactgc aaggggtttc
    51781 gcacttgctg acatcacagg ggctccggcc ccttcttctt aatctccttt aatatccgtc
    51841 acgcgcttct cccgacatac tgccctgaac ttacacagga gcacaatcat gtcacagacc
    51901 ccatccgata cttttaaatt gtcttatccc ttcaccactg ctgcaggcac cagaattgag
    51961 ctggttgaac tgaaacgcct gacggtaaaa gacctgaagc aggtgcgcaa aatcagcaaa
    52021 aacccggcag actgggacga accgctgatt gcccgcagta ctggtcttct cccggaggat
    52081 ctcgacaata tggatctggc tgattacctg cagttacaga aacgatttca gctcatcacg
    52141 gggatgggtg agagcaacca gggcgctgac gcaggcgcag gggctgctgg cgagatggtt
    52201 ccggtttcag ccgggggaga ttgatgccct cgatactgac gatctggaga tgtggctgga
    52261 gcaggctgaa gagcaaatca aaagcgagta cggcgacaaa tcatagtaca gacagccgcc
    52321 agtagcggct gttctgcgtt atcccctcac gtcttttcac cttccccgga ggttaaccac
    52381 tatgtcggga cagttttcag tcggcgttgt tatcggcggg atgattggca gcacattccg
    52441 ttctgcaatg agcggtaccc gccgtgcgct tgattccctg agcgatacct cacgccgcct
    52501 gcaggaacgt cagaacgctt taacccgtgc aacagaacgt tatggtcaac tgggttcttc
    52561 ccggatgcag catctcaaca gcgagctgct gcgggtaagc cgcaccatgg agcaaattga
    52621 gcgccagcag cgccgtctgt cagcggcatc cgctaccagt gatgcgctga aagctaaccg
    52681 catggcgctg tatggtcagg ggattgaagc gtatggcatg gcacagactg tttatcatac
    52741 ggtttcccct gccgttcagc agtccatgtc ttttcaggac aaaatgattg atatgtcgat
    52801 caccgcaaaa tatgacaata aaacgcggga tgcacttgcc ggacagataa aaggctgggc
    52861 gcttaaatac aatcagtatc aggatgagct gcaggaggcg atgggttcac tcatcagcga
    52921 caatattgat aatgtgtcag atatcggttt tctgatgccg gatattgccc gcgcggcaac
    52981 ggcaacacgc acgtctgctc aggactgggc aaaagtggcc gcagtctggc aaaactccct
    53041 gaaaggcgcg gccagagatt ttggtgccgt tcagaatatt atggcttatg ccggtgacca
    53101 ggggtcattt gaaatcccgg atcaggtcaa gtggatgcag tccctggccc caatgatggc
    53161 gggtattgcc agtggaaaag aggctgttgc tgaaatcggg gccagtctcc agatagcaaa
    53221 aatcggtgca ggttccaccg acgaagcagc caataatttt aaaaactttc ttaccaaaat
    53281 ttttgcccgc gatactcaga aacagtttgc tgatctgggt attgatttgc agggatctat
    53341 tgcgagttat aaagctgcgg ggatctctcc gattgaaggg atgttgagtg ttatagaacg
    53401 ttacctcaat gccaaaagcc ccgaagcgct ggccggcttc aaatcagcca tgaaaataaa
    53461 gaatgatacg gcaagagatg aggcacttca ggctctggcg aaaaactttg gtctgggcga
    53521 tatgttcgcg gatatgcagg tcatggcatt tatccgcccg atgctggcca acatggacag
    53581 atatcgagag atccgtgccg gtgctctcag ggctgcggat aacgatttgc ttgccagtgc
    53641 ttatgatcag cggctgaaat ctccccttga agccactaaa acacttatgg tcagcagtcg
    53701 cgatctggca attacgctgg gcgatcaatt agctccatct tttatttctc tgactcagga
    53761 cttgctccca ctcattcagg ggacaaaaca ctgggtggcg actcacccgc aatttgtcag
    53821 tggggctttt aagctcatca gtgcgctcct tgcgattaag atagcgactg ttggtctcaa
    53881 actggggctg aatctcctta tttccccctt tgtgaacgtc tggaaaactg ctgttttact
    53941 ccggaccaac tggcatcgac tgactaccgc actcggagaa ggtggcaaat tacgctggct
    54001 tgtcactgga ttcagccgac tgaccagcgg aggactgaaa ctcagcaaag ttctggcggg
    54061 cagcctcgtt cgcggtttta tgagcgctgc acgtgccgtt ctctggattg ggcgagcgct
    54121 gatgatgaat cccatcggtc tcgttatcac cgctgtcgcg gcagcagctt accttatcta
    54181 ccgcaactgg ggggcagtca gtggctggtt taaacagcgc tgggctgaca ttcaggaagc
    54241 ctttaacggc ggtatcgcgg gaactggtaa gctgctgatt aactggtcgc cggcaggcct
    54301 gctctataaa gcctttgcgg ctgcgctgaa atatttcggc gttactctgc cggcaaagtt
    54361 caccgacttc ggtggccatc ttatcgacgg tttgattaac ggtatcaaaa acaaatgggg
    54421 gtcgctcaaa tccagtgtaa ccggaatggg tgacagcatc agtagctggt ttaaacaatg
    54481 ctgggctgac attcaggaag cctttaacgg cggtatcgcg ggaactggta agctgctgat
    54541 taactggtcg ccggcaggtc tgctctataa agcctttgca gctgcgctga aatatcttgg
    54601 tgttgatctg ccggcaaagt ttaccgactt cggtggccat cttgtcgatg gtctgattaa
    54661 cggtatcaaa aacaaatggg agtcgctcaa atccagtgta accggaatgg gcgacagcat
    54721 cagtggctgg ttcagcgaaa agctgggcat tcattcgccg agccgcgtgt ttatgggctt
    54781 tggtgacaat atcgcgcagg gggccgccat tggcctgcag cggaccactc cgcttgcagc
    54841 tctggccggg cagcgaatgg ccagtgaact gctccccaga atgcccgtga gcattcaggg
    54901 gccagaaata cgggataaca cttcaggtgt tcgcttcagt atgccgttgc ccgatatcaa
    54961 tgggtttatg tcgtctgcta aaaatgcgat cggagccgta atcaatagtt tgtcttccat
    55021 gcccgctatt ccgctctcca cccgggcatc gattttacca gggcaacgtc tggcaaatga
    55081 gatgacaccg gatgttcccc gtatcccctc gcctgaaatc ctggctgccg gatattcagg
    55141 ccgtggtgca gctgcaaccg gcggtggaac gtctggtggt atccaggtca gctttaatcc
    55201 tcagtttttc ctcaatggca gggaaaccac agcgcctgca ggactgactg gtgccctgaa
    55261 tatgagtctg catgagctgg aaaaaatgct ggagcgtctg ctggctcaga aacaacgtaa
    55321 ggagtaccgc tgatgtttgc cgtactgggt gatattgagt ttgagctgat tacctactgg
    55381 gacggcttcg aggccacgtt cggcgtcgat tatgcggagc atgcccgcat cgggggtaag
    55441 cctggtctgc agttcgtcgg cgacaggctg gacgaaatcc agataactct ggttttccat
    55501 cagcattatt gtgtacccga tgtggagctg gcgagactgc gaacagccat gaaagcccat
    55561 caggcactgg cgctggtctt cggcaacggt gactatcgcg gctggttcgt gatcaccgat
    55621 gtgactgcaa ccagcgagca gactgacagt accggcaacg tgctggctgt cagtgccacc
    55681 gtgtctctcc gggaatacac cggtgacccg aaaaatcctc tgcaaccacc ggcaatacgc
    55741 acgaagctcc cgggtgtcgg ggcggtctcc ggtgccatac cttcaccttc aggggtggcg
    55801 cagttcatcc gcaacggcgt caactatgcg aaacaggcgc agtctgtact ccagaccact
    55861 atcagcgccg ttcgggtgac acagaaaatg aaggataacc ccgttgtcgc actgacccgt
    55921 gtgccggggc tgatgagcgg actgggtaat atctccgggg ctctggggaa aagtgttccg
    55981 gcgtttaacg cactctctga atccatgccc gatgccatca gtctggccag aacagccagc
    56041 gaagcagcca cgtatgtaca gcaggcacag tctgcgctga gtggtgtgga caaaagaaat
    56101 attgcaggtg ctctggatac cgtttccggg cagcttaacg ccgtcggcac agcattcaac
    56161 cgcatgtctc cgggattaag tgcaatggcc gccagaatac tgacgaggag tgtgtgatgt
    56221 ttcttgaaca tgttacccgt gacggagagc gctgggattc gctggcatgg cagtactacg
    56281 gtgacccgct gggctatccc cggattattg ccgccaatcc gcacgtggcc atcacgccgg
    56341 tgctgccctc cgggctgttg ttactgatcc cggtgattga ggctgaagaa gcccgtacag
    56401 aagaggatat tgccccatgg ctgagataaa cagcactgcg caagtcacat cagcgttaac
    56461 cggcgtcagc gatgtgctga caccggtgtt cactctgtgg tatctgcaga aaaacatcac
    56521 ctctgatatc gcgccttatg tcacccgtgt gacctggagc gataacatca aaaatgagtc
    56581 cgataccatt gaggtggagc tggacgacac cgatggccgc tggctggata aatggtatcc
    56641 gggcaagggt gacacgctga cgctgaaaat gggctatcag ggcgagaagc tgctgtcctg
    56701 cggtacgttc tctatagacg agatcgaagt gagttcgccc gcttccgttg tttctatccg
    56761 tggggtggcc acctcggtta acagtgccct gcggactaaa accagtcgtg gttttgagaa
    56821 caccacgctg gcagctgttg cggggcggat tgccagaaag caccggctga aactggtggg
    56881 cagcattgag tccatcagaa tcgaccgggt gacccagtat gctgaaaccg acgtgggttt
    56941 tctgcgccgg ctggccagcg agtatggtta tgcagtgaaa gtggtcagtg accagctgat
    57001 tttttctcat ctggccacac tgcgcagtca ggagccggtc aggcagttaa aaccgcagga
    57061 tgtggcccgc ttttccctgc gtgacaccat caaccgggtc tataaatctg caaaggtaaa
    57121 acaccagaaa agcagcagta aaaaactgat cgtctacgaa gctgatggtg gtacccgtga
    57181 aagcgacaaa aagctcaaag gtggtaaggt taccagcgct gactcactta aagttaacag
    57241 ccgcgtcagc gacccggaca gtgcccggat taaagcggat tcagcactgg ccagacataa
    57301 cgaataccag cagaacggct ccctgacgct gacgggaaca cctcaactga cagcaggcaa
    57361 caaaattgaa ctggtgagtt ttgggcagtt atccgggcca tggctcataa ccactgcccg
    57421 ccatgcgttt gaccgtaaca gcggctacac cacagagctg gaagtggcac gggggccagt
    57481 cacaagaggg aaaaaacaaa aaactcagaa actcacggtt tatcacccgg atggcagtac
    57541 atcgacggtg attaaggaga agaaaaaatg actggtgtca ctcgtcaggt cggtacggtc
    57601 agtgccgttg atgccgacag ggttcaggcc cgcgttcgtc tgcctgaatg cgataacctg
    57661 cgcacaaact ggcttaacgt gctgcagcgc aatacccagg ataacaaaga ttactggctc
    57721 cctgacgtgg gggagcaggt tgaggtgctg ctcgatgcca acggcgagga tggtgttatt
    57781 ctgggcgcgg tgtattcaga cgtcgataaa ccaccgttca gtgacaaaaa tatccgggga
    57841 acccggtttg ctgatggtgc agagtacagc tacaaccgga agacgcacac tctgatcatc
    57901 cggggcggca ttgagcatat tgtcattgag tgtggtgctg atgtggtatt gaaaacacag
    57961 aaagccacga ttgacgcacc ggaaaccgaa cttaccggag atctgcgtgt caggggtaag
    58021 ctgatttacg aaggaggcat ggcgggttct ggtggtgaag gtgttaccgc gaccatccat
    58081 ggcaatatcg agattaaagg gaatgtccat gccacgggca gtatgttgtc tgatggcgaa
    58141 aactccagcc accactccca ctgagctttt taaacgcctt taatatcagc gttcccgcac
    58201 gggggcaata ctgcccccat gaaaacaacc tcagtattct ggcaaccagc cctgcaggct
    58261 cccggcgaaa ttgtccgggg gctggatgat atccggcagg cgattcaaat tatcctgcgg
    58321 actccccgcg gcagcgaccc gcatcgcccg gagttcggca gcaatctgca tctttatatc
    58381 gactggcctg tagaccgggc cattccgcat gtggtgcgcg aatccgtcga tgccatccgg
    58441 cgctgggagc cccgctgcca gcttatgtca gttaaacccg ccgtcgacgg cgaacatctt
    58501 acgctccggg tgagctggaa aggctcagac ggacagaccc ggactcagga gctgctatgg
    58561 cgctgacaga acccgatttt attgaacgcg atgccgacaa aatcacggca gaaatgattg
    58621 cgaagtatga agcggatacc ggcaaaacgc tgtacccggc acaggcagaa cgtctgctga
    58681 ttgatctgtg ggcctatcgc gaaatgctgg tcagggttgc ggtacaggag gcagcaaagc
    58741 agaatctggt cgcctttgcc cgtgagccga tgattgatta cctcggtgaa ctggtcggtg
    58801 tataccgtct tgccgcgcag cctgccacca ccacgctcca gttctccgta gatgaggcac
    58861 tggccattga tgtgctgatc ccggcaggca cccgcgtcag cgcttccgac agcgttattt
    58921 ttgccaccga tacagatgtg gtactgaagg ccggattgct gctggtcaat gtcacggcca
    58981 cctgtaccga acccggtacc gctggtaacg gctggcaacc tgcgcaggtc agtcagttac
    59041 tcgatgagat tgataacgtt aacctgctgg tgagcaatct gatggccagt tccggcggtt
    59101 cagaacagga agacgatgac aggctccggg agcgtatcag gctggccccg gagtcattca
    59161 ccaatgccgg aagccgtggc gcataccgct ttcatgccat gcaggcccat cccaacattg
    59221 tcgatgttgc tgtgctttcc ccggttcccg ggaccgtaga tctgtatccg ctgctcagta
    59281 ccggtctgcc ggacggcggt gttctcacgc tggtagagag tttctgctct gatgagaaag
    59341 tcaggccact cactgataca gtgcgggcta aaacaccagt gaaggtggat tacaccattg
    59401 aagccaggat tacgatctat cgtgatcagg atgccaggtc tgtaaaagat gccgctaaca
    59461 gcgccataca gaactgggtg gcatcacgtg ccgccacgct ggggcgcgat attgtcccca
    59521 gccagattat cagtgcattg tccgtttccg gggtgtacca ggttgaactg gtgacaccgg
    59581 cactgcgggt ggtggcagaa aacgaatggg caaactgtac ggcaatcact cttaacatga
    59641 ctggagtgtc tgatgactga gccattacaa ctcccgccac cgcttgaggg tgatatcagt
    59701 ctcaggacgc tgggaagact ggcaggacgg ctggataaca tcgacctgag cgtactgatg
    59761 gtctttctcg tcgatatcgt cgacagttcc gcgctgccat ggctgggcga gcagttctca
    59821 ctgtctggcg atggctggga gctggcggaa tcggacgatg ttcgccgcat gcttataaaa
    59881 gcagccatcg aactgcaccg gtataaaggg acgccgtggt caatccggga agttatccgc
    59941 cgttttggct ttggtgaagt ggatctgatt gaaggcacag gtcgtctcag ttacgacggc
    60001 aatcgcagct ataacggact gtttgttcat ggagatgcag ccgcctgggc agtttatcgc
    60061 gttattctga aacagcccat tactaacgat caggccgcga tgctgcgtca gacgctggct
    60121 gcatttgcac cggcccgctg ccatctggcc agcctggagt atcagtctgt ggccattcgc
    60181 tataacaata ccgccatcca tgatggcagt tataaccacg ggagcagttg attatgggaa
    60241 acctgaatga aacagaaaag tgggaagaaa atatctatca actggagaca tcagatccgg
    60301 ttctgggtgg tgcagacggg atatcaaatc gagccccccg gcaactggca aacaggacga
    60361 aatggctgaa gaagaaaacg gaggaagccg cacagtcact ggctgaacac gtacgttccc
    60421 gtaaccaccc ggacgcgaca ctgacagcta aggggttcac ccaactgagc agtgccacca
    60481 acagcacctc tgaaacgctg gccgccacac caaaagcggt caaggctgca tacgacctgg
    60541 cagccggcaa ggcgcctgcc agccacactc acccgtggag tcagataacg ggagtgcctg
    60601 cggcttcact gacggcaaaa ggcaccgtac aactgagcag cgccacggac agtcaatcag
    60661 aaactgaggc tgccacgccg aaggcggtca aggctgcata cgaccttgca gccggcaagg
    60721 cgcccgtcag ccacactcac ccgtggagtc agataacggg agtgcctgct gcctcgctga
    60781 cggcaaaagg caccgtacaa ttgagcagtg ccatcaacag cacgtctgaa atactggccg
    60841 ccacaccgaa agcggtcaag gctgcatacg atctggcaaa cggaaaacaa ccggcagatg
    60901 ccacgctcac tgctctggca ggacttgcca ctgcagcaga tcggttgcct tattttaccg
    60961 gagcagaccg cgcagagctg gcaaccctta cagctattgg tcgcgctatt atcgctaagg
    61021 gcagtataaa agatgtcctc aattaccttg gtttggggga aggctcggcg ctgcccgttg
    61081 gtgtacctgt tccgtggccc actgccacac cgccagcagg ctggttaaag tgcgacgggc
    61141 gcgcctttac aaaagaacaa tatcctgttc tggccagagt ctacccgacc ctccgtcttc
    61201 ccgatttacg cggtgagttt atccgtggat gggacgacgg gcgaaaagtg gatacagggc
    61261 gcgatctgct ttcacggcag gatggtacaa gtttttctca ttacgcaggt aattttgaca
    61321 ttgggtctgg tcattcaatc aataactatg accaaatcgt tgacaaccaa cctggattct
    61381 cccgtttttc atttgcaggt ccttcgcgag gcgatggggt taattatgtg accattcgtc
    61441 cccgtaacat tgcgtttaat tacatcgtaa gggcggcata gaaacgttgg tttgggggaa
    61501 ggctctgcat tgcctgttgg tgtaccggtt ccatggccca ccgccacacc gccagcaggc
    61561 tggttacagt gcaacggagc tacgttcacc aaagaacaat atcctgttct ggtcagagtc
    61621 tacccgaccc tccgtcttcc cgatttgcgc ggtgagttta tccgtggatg ggacggtggt
    61681 cgcaaggttg ataccgggcg tgcgctactt tcatttcagg aagggactat cgtatccggt
    61741 tttgatgata atgataccgg agatatcagt tcgctcggct ctacacaata cggatttggt
    61801 gacgctttaa cctctaatca actgggggtc ttaaatggca aaaagtggat atttgatgca
    61861 tcctctaaag gtgcacaaag atatgactgg tgggcttatg tatcagctcg cccccgcaat
    61921 atcgcattta actatatcgt gagggcggcg tgataccgtt cagccggcaa gcgccagtgg
    61981 cgcttccggc cagctgattt caggtgcctt gctgatatcc agtgcgttca gctcgtcgat
    62041 ataatccagc acggcgttca gttttgccgt ttcttctggt ttcagactgc gaccagcacg
    62101 cagtttgagg ttgataagat cgacggacgc catcgcctgt ttcacccgtt gctggcgttg
    62161 ctgtctggca acctccagtt ctgcggctcg ctgccgttca gtatcggtta cccacgcctc
    62221 accgttccag gtatcccatg gcgtggctgg ctgttttgtg gtggtgtccg ccgggtaatc
    62281 acccggttgg gtaatcagaa cggggttacc gttctccgtg ctgtataccg tctcgccgcg
    62341 gtgatcaggc acatgctccc acccgttcag cgtggcagtc cggcaaacca cgtaacccga
    62401 aattgcttcc ggcggtgcat cggcgcacga atgtgccgga gtacccaccc caagggcgag
    62461 atactccaca gaggatgagg tgtattcccg ggtttcctcg tcatagtgat atacagtgat
    62521 atcgcccgct acagtggcga taccgttttc atccagaact gcagtgttac tcattaaact
    62581 gccctcacaa tgtaattaag tgcaatgcta cggggtctga cagtgatacc cttcgcatac
    62641 ccgcttctga tagaggtcag aatagtagcc tgcataccat tgtctgacat tgccacgaca
    62701 ggatcgctga atgttgtccc gtccggcatt ttaaagctgc cctcacgaag gtctgtaatg
    62761 gaatcttcat tgttgaatcc catgccgact atccccgagg ttcccgtagt gtcctggtta
    62821 taataatcca gcattgctgt tcttaacagc gttgcccctt gtgcggacag caacttacgt
    62881 cctgtatcaa ctttgcgccc gtcgtcccat ccacggataa actcaccgcg taaatcggga
    62941 agacggaggg tcgggtagac tctggccaga acaggatatt gttcttttgt aaaggcgcgc
    63001 ccgtcgcact ttaaccagcc tgctggcggt gtggcggtgg gccacggaac aggcacacca
    63061 acgggtagcg ccgagccttc tcctaaacca acgtttatct ctcaaatcct ccccacatta
    63121 tctcagtact ttaaccactc aaaagggagt atttttaatg ctgattggct acatacgtgt
    63181 gtcaacaaat gaccaaaaca cagatttgca acgcaatgca ttaatgtgcg caggatgtga
    63241 acagattttt gaggacaaaa tgagcggaac caagtcggaa cgaccaggcc tgaaacgcgc
    63301 tttaaagtgc cttaaaagag gggatacatt ggtggtctgg aagctggatc ggttgggtag
    63361 aagaatgaaa cacctcattg ctctcacaga agagctacgc gcaaaaggtg tcaattttcg
    63421 cagtctgacg gattcaatcg ataccagcac tccgatggga aggttctttt ttcatatgat
    63481 gggtgcactg gcagaaatgg agcgtgaact gatagttgag cgcacgctgg ctggactggc
    63541 tgctgcgcga gcacaaggaa gagttggagg acgtcgcccg aaactgacga aggagcagca
    63601 tgagcagatc gcaagattgc tccagaaagg gtatgacaga aagcggctgg caattatcta
    63661 tgatattgga ctgtcaacga tctaccgcta tcaccctgtt gggactgtca taacgcaacc
    63721 tgaaatgtaa ttctttttca aataatgaaa cgccgcgtgg atgccattta tcgcacaaga
    63781 tagagtgcat tt
//
LOCUS       DAPQZD010000006        61799 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPQZD010000006 DAPQZD010000000
VERSION     DAPQZD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.006"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(78..896)
                     /gene="cof"
                     /locus_tag="RO454_000418"
     CDS             complement(78..896)
                     /gene="cof"
                     /locus_tag="RO454_000418"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDY0932302.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            996..2696
                     /locus_tag="RO454_000419"
     CDS             996..2696
                     /locus_tag="RO454_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDY0932303.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2761..3456
                     /gene="queC"
                     /locus_tag="RO454_000420"
     CDS             2761..3456
                     /gene="queC"
                     /locus_tag="RO454_000420"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDY0932304.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(3508..3906)
                     /gene="fadM"
                     /locus_tag="RO454_000421"
     CDS             complement(3508..3906)
                     /gene="fadM"
                     /locus_tag="RO454_000421"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDY0932305.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4024..4395)
                     /locus_tag="RO454_000422"
     CDS             complement(4024..4395)
                     /locus_tag="RO454_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDY0932306.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            complement(4546..6417)
                     /gene="ppiD"
                     /locus_tag="RO454_000423"
     CDS             complement(4546..6417)
                     /gene="ppiD"
                     /locus_tag="RO454_000423"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDY0932307.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6609..6881)
                     /gene="hupB"
                     /locus_tag="RO454_000424"
     CDS             complement(6609..6881)
                     /gene="hupB"
                     /locus_tag="RO454_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDY0932308.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7090..9444)
                     /gene="lon"
                     /locus_tag="RO454_000425"
     CDS             complement(7090..9444)
                     /gene="lon"
                     /locus_tag="RO454_000425"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDY0932309.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9632..10906)
                     /gene="clpX"
                     /locus_tag="RO454_000426"
     CDS             complement(9632..10906)
                     /gene="clpX"
                     /locus_tag="RO454_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDY0932310.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11032..11655)
                     /gene="clpP"
                     /locus_tag="RO454_000427"
     CDS             complement(11032..11655)
                     /gene="clpP"
                     /locus_tag="RO454_000427"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDY0932311.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11901..13199)
                     /gene="tig"
                     /locus_tag="RO454_000428"
     CDS             complement(11901..13199)
                     /gene="tig"
                     /locus_tag="RO454_000428"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDY0932312.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13543..13860)
                     /gene="bolA"
                     /locus_tag="RO454_000429"
     CDS             complement(13543..13860)
                     /gene="bolA"
                     /locus_tag="RO454_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDY0932313.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14165..14743
                     /locus_tag="RO454_000430"
     CDS             14165..14743
                     /locus_tag="RO454_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0932314.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14787..16262
                     /gene="ampG"
                     /locus_tag="RO454_000431"
     CDS             14787..16262
                     /gene="ampG"
                     /locus_tag="RO454_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDY0932315.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16722..17669
                     /gene="cyoA"
                     /locus_tag="RO454_000432"
     CDS             16722..17669
                     /gene="cyoA"
                     /locus_tag="RO454_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDY0932316.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17691..19682
                     /gene="cyoB"
                     /locus_tag="RO454_000433"
     CDS             17691..19682
                     /gene="cyoB"
                     /locus_tag="RO454_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDY0932317.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19672..20286
                     /locus_tag="RO454_000434"
     CDS             19672..20286
                     /locus_tag="RO454_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDY0932318.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20286..20615
                     /locus_tag="RO454_000435"
     CDS             20286..20615
                     /locus_tag="RO454_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDY0932319.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20627..21517
                     /gene="cyoE"
                     /locus_tag="RO454_000436"
     CDS             20627..21517
                     /gene="cyoE"
                     /locus_tag="RO454_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDY0932320.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21666..23030
                     /locus_tag="RO454_000437"
     CDS             21666..23030
                     /locus_tag="RO454_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0932321.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(23158..23649)
                     /gene="yajQ"
                     /locus_tag="RO454_000438"
     CDS             complement(23158..23649)
                     /gene="yajQ"
                     /locus_tag="RO454_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDY0932322.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            23817..24728
                     /gene="panE"
                     /locus_tag="RO454_000439"
     CDS             23817..24728
                     /gene="panE"
                     /locus_tag="RO454_000439"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDY0932323.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            24691..25281
                     /gene="yajL"
                     /locus_tag="RO454_000440"
     CDS             24691..25281
                     /gene="yajL"
                     /locus_tag="RO454_000440"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDY0932324.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(25335..26783)
                     /gene="thiI"
                     /locus_tag="RO454_000441"
     CDS             complement(25335..26783)
                     /gene="thiI"
                     /locus_tag="RO454_000441"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDY0932325.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            26989..27231
                     /gene="xseB"
                     /locus_tag="RO454_000442"
     CDS             26989..27231
                     /gene="xseB"
                     /locus_tag="RO454_000442"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDY0932326.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            27231..28130
                     /gene="ispA"
                     /locus_tag="RO454_000443"
     CDS             27231..28130
                     /gene="ispA"
                     /locus_tag="RO454_000443"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDY0932327.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            28155..30017
                     /gene="dxs"
                     /locus_tag="RO454_000444"
     CDS             28155..30017
                     /gene="dxs"
                     /locus_tag="RO454_000444"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDY0932328.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            30072..31046
                     /gene="yajO"
                     /locus_tag="RO454_000445"
     CDS             30072..31046
                     /gene="yajO"
                     /locus_tag="RO454_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDY0932329.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(31100..31618)
                     /gene="pgpA"
                     /locus_tag="RO454_000446"
     CDS             complement(31100..31618)
                     /gene="pgpA"
                     /locus_tag="RO454_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDY0932330.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(31596..32573)
                     /gene="thiL"
                     /locus_tag="RO454_000447"
     CDS             complement(31596..32573)
                     /gene="thiL"
                     /locus_tag="RO454_000447"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDY0932331.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(32651..33070)
                     /gene="nusB"
                     /locus_tag="RO454_000448"
     CDS             complement(32651..33070)
                     /gene="nusB"
                     /locus_tag="RO454_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDY0932332.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(33090..33560)
                     /gene="ribE"
                     /locus_tag="RO454_000449"
     CDS             complement(33090..33560)
                     /gene="ribE"
                     /locus_tag="RO454_000449"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDY0932333.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(33649..34752)
                     /gene="ribD"
                     /locus_tag="RO454_000450"
     CDS             complement(33649..34752)
                     /gene="ribD"
                     /locus_tag="RO454_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDY0932334.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(34756..35205)
                     /gene="nrdR"
                     /locus_tag="RO454_000451"
     CDS             complement(34756..35205)
                     /gene="nrdR"
                     /locus_tag="RO454_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDY0932335.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            35356..35895
                     /locus_tag="RO454_000452"
     CDS             35356..35895
                     /locus_tag="RO454_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDY0932336.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            36194..37078
                     /gene="tsx"
                     /locus_tag="RO454_000453"
     CDS             36194..37078
                     /gene="tsx"
                     /locus_tag="RO454_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDY0932337.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(37116..37463)
                     /locus_tag="RO454_000454"
     CDS             complement(37116..37463)
                     /locus_tag="RO454_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDY0932338.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(37593..38564)
                     /gene="secF"
                     /locus_tag="RO454_000455"
     CDS             complement(37593..38564)
                     /gene="secF"
                     /locus_tag="RO454_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDY0932339.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(38575..40422)
                     /gene="secD"
                     /locus_tag="RO454_000456"
     CDS             complement(38575..40422)
                     /gene="secD"
                     /locus_tag="RO454_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDY0932340.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(40450..40782)
                     /gene="yajC"
                     /locus_tag="RO454_000457"
     CDS             complement(40450..40782)
                     /gene="yajC"
                     /locus_tag="RO454_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDY0932341.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(40805..41932)
                     /gene="tgt"
                     /locus_tag="RO454_000458"
     CDS             complement(40805..41932)
                     /gene="tgt"
                     /locus_tag="RO454_000458"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDY0932342.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(41987..43057)
                     /gene="queA"
                     /locus_tag="RO454_000459"
     CDS             complement(41987..43057)
                     /gene="queA"
                     /locus_tag="RO454_000459"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDY0932343.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            43150..43731
                     /gene="acpH"
                     /locus_tag="RO454_000460"
     CDS             43150..43731
                     /gene="acpH"
                     /locus_tag="RO454_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDY0932344.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(43736..45553)
                     /gene="malZ"
                     /locus_tag="RO454_000461"
     CDS             complement(43736..45553)
                     /gene="malZ"
                     /locus_tag="RO454_000461"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDY0932345.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(45709..47082)
                     /gene="proY"
                     /locus_tag="RO454_000462"
     CDS             complement(45709..47082)
                     /gene="proY"
                     /locus_tag="RO454_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDY0932346.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(47158..48477)
                     /gene="brnQ"
                     /locus_tag="RO454_000463"
     CDS             complement(47158..48477)
                     /gene="brnQ"
                     /locus_tag="RO454_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDY0932347.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(48884..50179)
                     /gene="phoR"
                     /locus_tag="RO454_000464"
     CDS             complement(48884..50179)
                     /gene="phoR"
                     /locus_tag="RO454_000464"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDY0932348.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            complement(50237..50926)
                     /gene="phoB"
                     /locus_tag="RO454_000465"
     CDS             complement(50237..50926)
                     /gene="phoB"
                     /locus_tag="RO454_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDY0932349.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            51116..52318
                     /gene="sbcD"
                     /locus_tag="RO454_000466"
     CDS             51116..52318
                     /gene="sbcD"
                     /locus_tag="RO454_000466"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDY0932350.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            52315..55458
                     /gene="sbcC"
                     /locus_tag="RO454_000467"
     CDS             52315..55458
                     /gene="sbcC"
                     /locus_tag="RO454_000467"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDY0932351.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            55584..56768
                     /gene="araJ"
                     /locus_tag="RO454_000468"
     CDS             55584..56768
                     /gene="araJ"
                     /locus_tag="RO454_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDY0932352.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(57037..57945)
                     /gene="mak"
                     /locus_tag="RO454_000469"
     CDS             complement(57037..57945)
                     /gene="mak"
                     /locus_tag="RO454_000469"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDY0932353.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            58070..58981
                     /gene="rdgC"
                     /locus_tag="RO454_000470"
     CDS             58070..58981
                     /gene="rdgC"
                     /locus_tag="RO454_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDY0932354.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(59062..59142)
                     /locus_tag="RO454_000471"
     CDS             complement(59062..59142)
                     /locus_tag="RO454_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932355.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(59139..59480)
                     /locus_tag="RO454_000472"
                     /pseudo
     CDS             complement(59139..59480)
                     /locus_tag="RO454_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(59628..59912)
                     /gene="ppnP"
                     /locus_tag="RO454_000473"
     CDS             complement(59628..59912)
                     /gene="ppnP"
                     /locus_tag="RO454_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDY0932356.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(59984..60661)
                     /locus_tag="RO454_000474"
     CDS             complement(59984..60661)
                     /locus_tag="RO454_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDY0932357.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(60919..61110)
                     /gene="yaiA"
                     /locus_tag="RO454_000475"
     CDS             complement(60919..61110)
                     /gene="yaiA"
                     /locus_tag="RO454_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDY0932358.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(61160..61684)
                     /gene="aroL"
                     /locus_tag="RO454_000476"
     CDS             complement(61160..61684)
                     /gene="aroL"
                     /locus_tag="RO454_000476"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDY0932359.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT        14840 a        16500 c        15429 g        15030 t
ORIGIN      
        1 tttaagcacc acggcacaaa atgacaaatt caacgcaaaa aaaaccgggc aattgcccgg
       61 tgctggaagg gatctcgtta ttcgggggaa taaggtagat gtggatagtc cagccagtgc
      121 gtcaaatagt gagagacagc ctgatttcgg caatgtccaa tcaccggtaa atgcgggagc
      181 tccgcgcgca gttgcggcat cgcattgccc ataataaatc cgctaccgac gctgcctaac
      241 atttcgcgat cgttcatcgc atcaccaaag gccatacaat cgcgtaacga taaacctaaa
      301 tgttgggtca gcaccgtcaa tgcagcgcct ttattgcagc ccaccggcag cacttcgagg
      361 caatccgtgg cggaaaaaca taaatgtgca cgctcgccta atgcttcgta tagctggatc
      421 tgcaagcgtg taagatcgtc gtgatcgcca cagaagcaga tcttggtgac gctgccgagt
      481 ggcatttttt tgacatcgat tatctgataa cgaaaaccgc tatagacaaa tgcctgcaac
      541 aacgcaggga tctctttccc ggtaaaccaa ccatcgtcat tgaagatatg catgcttgct
      601 cgggtatccc attgctgata cagcaccagc tccgcgacat ccgcaggtaa atcatcacga
      661 tgtaaaagtt caccttccag agaatgcacg cgcgttccgt tgccagtaat caaatatgca
      721 tccagcgata gcgccccgag aatatgctgc atctccagcg catgacgccc cgtggcaaaa
      781 gtgagggtaa tgtcgcgttc acgcagccgc gccaaagtag agagggtttt ctcacctaaa
      841 tgatggtcag gcatcaataa agtgccatcc atatcaaatg ctgccagacg agccatttct
      901 ttctccactc tgtgacacag ttaatggtga ttgagtatca cctgatatat acggaagtaa
      961 tagtgaatag ttaaataata ttgttccggg tttatatgcg actgctcaac cgtcttaacc
     1021 agtatcaacg tctgtggcaa ccttccgccg gaaagccgca aaccgtcacc gtcagcgaac
     1081 tggccgaacg ctgtttttgc agcgaacgcc atgttcgtac ggtgttgcgt caggcacagg
     1141 aggcgggatg gctggagtgg caggcgcagt caggacgcgg aaagcgcggt caactacgct
     1201 ttctggtcac gccagaatcg ctacgcaatg cgatgatgga acaggcgctg gaaaccggaa
     1261 agcagcaaga tgtgctggag ctggcgcaac tggccccagg tgagctgcgc actctgttac
     1321 agccgtttat gggcggacaa tggcaaaacg atacgcccac gttgcgtatt ccctactatc
     1381 gcccgctcga accgctacaa ccgggctttt tgcccggccg tgccgagcag catctggccg
     1441 ggcagatatt ttccggcctg acccgcttcg ataataatac tcagcgcccg attggcgatt
     1501 tagcacatca ctgggaaacc tctactgacg ggttacgctg ggacttttat cttcgttcaa
     1561 cgctacactg gcataacggc gatgcagtaa aagcctcaca cttacaccag cgattattga
     1621 tgctgttaca actgccagca ctggatcaat tatttattag cgtgaagcgt attgaagtca
     1681 cccatccgca gtgtctgacc ttctttttac atcgccccga ttactggctt gcgcaccgac
     1741 tggcgagcta ttgcagccat ctggcgcatc cgcaattccc actgatcggc acaggtcctt
     1801 ttcgcttaac acaattcaca gcggaactgg tgcgcctgga gagccatgat tattaccatt
     1861 tacgtcatcc gctgcttaaa gcggttgagt actggataac tccgccgctt ttcgaaaaag
     1921 atttgggaac cagttgtcgg catcccgtgc aaatcaccat tggcaaaccg gaggagctgc
     1981 aacgggtcag ccaggtcagt agcggcatca gtttaggttt ttgctattta acattgcgca
     2041 aaagtctccg actgtccctc tggcaggcgc gaaaagtaat ctccattatt catcaatccg
     2101 gtttattaca aacgttagaa gtcggagaaa acctgatcac cgccagtcat gcattactgc
     2161 ctggctggac tattccgcat tggcaggtac cggatgaagt taaactaccg aaaaccttga
     2221 cgctggttta tcacctaccg atagaacttc ataccatggc agaacgccta caggcgacac
     2281 tggcagcgga aggctgtgaa ctcacaatta tttttcataa cgcaaaaaac tgggacgaca
     2341 cgaccctact ggcacacgca gacctcatga tgggcgacag attaattggc gaagcaccgg
     2401 aatataccct ggagcaatgg ctgcgttgcg atccactgtg gccacatgtt ttcgacgctc
     2461 cagcatatgc acatttgcaa tcgacactgg acgcagtgca agtaatgcct gatgaggaaa
     2521 accgatttaa tgccctgaaa gcggttttta gccagttaat ggcagatgcg acgctgacgc
     2581 cgctgttcaa ctatcactat cgcattagcg cccctcccgg cgtgaacggt gtgcgactga
     2641 caccgcgcgg ctggtttgaa tttaccgaag cctggcttcc cgcgccgtcg caatgaatag
     2701 ctggtccggg ttaatcgcag gcgttaccat aacgctttta ttgttcaagc aggattatct
     2761 atgaaacgtg ctgtcgttgt gttcagtgga ggccaggatt ccaccacctg tctggtgcag
     2821 gcattacaac aatatgatga agtccattgc gtgacgttcg attacggtca acgccatcgc
     2881 gcagaaatcg acgtggcacg cgaactggcg ctgaaactgg gggcacgcgc gcataaggtt
     2941 cttgatgtaa cgctgctcaa cgagctggcg gtcagcagcc tgacgcgtga cagcattccg
     3001 gtgcctgatt atgaacctga agccgatggt atcccgaata cgtttgtccc agggcgtaat
     3061 attttgttcc tgacgctggc ggcaatatat gcgtatcagg taaaagcaga agccgtaatt
     3121 accggcgtct gcgaaacgga tttctccggc tacccggatt gtcgcgatga gtttgtgaaa
     3181 gcactaaacc atgccgtcag tttgggcatg gcgaaagata ttcgttttga aacgccgctg
     3241 atgtggattg ataaagcgga aacctgggcg ctggcagatt attacggcaa gctggattta
     3301 gtccgtaacg aaacgctgac ctgttataac ggcattaaag gcgacggttg cggtcattgt
     3361 gcggcgtgta atttacgtgc caacggtttg aatcattatc tggccgataa accgacggtg
     3421 atggcagcga tgaagcagaa aaccgggttg aagtaattat tccgggtgtc gccggatgcg
     3481 gcttgagcat ccggcaccac aaaacgttta cttaaccatc tgctccagct tttcgcgcaa
     3541 ttccccttcc agagctaatg ctttctgcgt tttaagatca atacaaacaa acgtaataag
     3601 cgcatccgct accacctgtc cttccggctc cagtgtaatg acctggctta agatgccact
     3661 tttaccgttt aattgctgca actgactggt aatggttaac aggtcgctta ataccgccgg
     3721 gcggcgatag ttaatattga tattgaccac gacgaaagcg atgttatggg ccgtcatcca
     3781 gtgaaaactg tcgctatttt ccaacccatg ccagcgggct tcttcgagaa attcaaggta
     3841 gcgggcgttg tttacgtgct ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt
     3901 ttgcatagcg caataacctt acgttgttgt tataagaact gttataagaa ctgttataag
     3961 tacagaggtc ataactggta tgacctcttc agtctggcaa aaaattgcca ctatgcaaat
     4021 taattacagg gttaataccg ccatattacg ttccaccagc gaattgccca tccccggcac
     4081 ctgctttaga tcctccaccg ttttaaacgg accgtactct tcgcgataac tgacaatcgc
     4141 ctgcgctttc ttcaggccaa cgccattcat cgcgcgggct aactcttccg cgctggcatt
     4201 attaatgctg acccgggtgc cttcttcgtc actggctttc gccggtactg ctgctttact
     4261 ttgtgctgca ggagcttccg ctttggtttc taccgttgtc ggttttgccg caggagctgc
     4321 cgccagagca ctatgagaca ttccggcaca ggccagggac agggtaatga gcagtgcttt
     4381 aattccgtgt ttcatactgt tttctccttg tttgttaaca gtggaatcac cttaacggca
     4441 gtgaaaacag taaacaaatg gcaatgttca gaaatggaaa aggccgcgaa agcgaccttt
     4501 tcttgttaca gctgcaaagc atttttttgc gaggcggctt caggattatt gctgttccag
     4561 cgcatcgcca attttgattt tcgcctcttt acgcaggtta ctcatcagag cttcaaagac
     4621 gatttgtgcg ttgttctggg tgataccctg caccatcgct tttttctgat cttccggcat
     4681 tgaaccttgt ttcacttcat ccagcgccag cagaaccaca ttaccttgca tatcggtcgc
     4741 cataccgtag ctcggtttgt ctttcgctgg cagcggcagt gcaaacgcag cctggctaat
     4801 cgggtcacga ccggaacggc ttaaggtttt cggctcgcca aatttcagac cggcagcctg
     4861 catcgcttcc gcacctttgc cggctttcaa atcaaccagc agtttctcag catccacttt
     4921 cgcctgttgt tcagctttgt tgtgctgaac cagtgcctta acttgttcct gaacatctgc
     4981 caacggtttc accgcttccg gtttgtgctc gctgatgcgc agcacgaatg cgcggtcgcc
     5041 gtctacggtg atgatgtcag agttgatgcc cggcgcgccg ttttcaccta ccagaccgcc
     5101 gttgaagata gcgtcggcaa ctggcttgaa gttcaactct tccggcaggt tatctttgct
     5161 gaaccaaccc gtctgagtgg ctttaacgcc ggcagcttgc tctgcaccgg ccagagactc
     5221 ggtgtcgttg cttgccgcat cgctcacttt ctgctgcagc gcgtagtacg catcgagggc
     5281 tttttcgtgt ttcactttcg ccgcaatgtc gtcacgtact tcgtctaacg atttcacttt
     5341 cgctggctga atgtcgtcca gacgtacaat caggaaaccg accgaagatt tgataacacc
     5401 agacagttgg cctttttctt tcagacccgc atttttcagt tcgtccggga tagtggcatc
     5461 ttctaaccaa cccatatcac cgccgttacg ggcagagata atatcggcag atttttcttt
     5521 ggctaatgca gcaaaatcac cgcccttatt cagctcatca agtaccgctt tcgcttcatc
     5581 ttcagttttg gtctggatga tgctgtaacg ggtacgctgc ggctgggtga attgatcctg
     5641 atgctgatcg tagtagctct ggatatccgc atcgctaacc ggttgctgca tcgtcgcggc
     5701 atccagcttg atgtaactca cgcggaattg ttccggcgtc atgaaattgt ttttgttttg
     5761 ttcgtagtag ctggcaattt cctgttcggt cacaggctgc ttcgccgcca gcgcgttaac
     5821 atcgatagcc gcttcacgca ccacgcgttg ttgagctacc agtgccgcca gctcgtcggt
     5881 ttcacctttc agcataaaat cggtaccggc aacgccgtta atcagctgtt gggtagtgag
     5941 ctggttacgc agcgcctggg cgtactgatc ggcggtcatc cccatctggt tgaggatacc
     6001 gttatagcgg ctgttatcaa atttgccgtc aacctggaag gctggggtcg cgaaaatcgc
     6061 ctgtttaacc tgctcatcgc tgatacccag tttcagctca cgagcgtact gatccagaag
     6121 cgcctcgtcg atcagacgat tcagcacctg ttgacgcagg gttttcatat agccttcgtt
     6181 cgctgccagc tcggagtatt gatcgcccag ctgttgctgc atgcgattac gctcgctgtt
     6241 gaaggcgttc tcgaattgcc cacggctgat ttcctggtca ttcacttttg cggcgtagtt
     6301 attgcctccg ccaatcaggt aaccactcac gccggtcaat atgaacgaca cgataatgat
     6361 accgaaaata atcttgagca cgagactgtt tgcagccgtg cgtaagctgt ccatcatggt
     6421 gtaacaacac tccgctgtag atgactgtgt acctcacgca acgtatcgat gcgcgcaaag
     6481 gcctattgtg acaagaaacg ggggcaattg tcagcccaca agacgcagaa agtcaccgaa
     6541 tacaaataaa aaaggcacat cagtagatgc gcccttgaac ttcgtcacat ccccactggg
     6601 gacaacgctt agtttaccgc gtctttcagt gctttacctg cacggaagct cggtacttta
     6661 gcagcagcga tggtgatctc tttaccggtc tgcgggttgc ggccagtacg ggcagcacgt
     6721 tccttaacgg caaaagtacc aaaacctacc agtgctacat catccccttc tttcagagat
     6781 tcagttacgg aagcaataat agcatctaac gcacggccag ccgcagcttt agagatatca
     6841 gcccctgcag caatcttgtc gatcaattga gatttattca ctcttctctt cctctttata
     6901 atttatatcg cacttgaatc cttcaaggta cgaacgcgca gcagttatat caggccagcc
     6961 atccccttac aacacccctt aataagggca agcccgatcc gccatctaac ttagcgagac
     7021 aaaaaaaggc tggcaagccc gaattagcct gccagccctg tttttattag tgcattttgc
     7081 gcgaggtcac tattttgcag tcacaacctg catgccagac ggttcatttt gtagcgccag
     7141 agtcagaact tcctcaatgc gcttcacagg atgaatgtcc agatcggcaa ttacgttgtc
     7201 aggaatctct tccagatcgc gtttattttc gaacggaatt aacactgttt taatcccgcc
     7261 gcgatgcgct gccagtagtt tttctttcaa accaccgatc ggcagtacct gaccacgcag
     7321 agtgatctca ccggtcattg ccacatcggc acgaaccggg ttaccggtca ggcaagaaac
     7381 cagcgcggtg cacatggcaa taccggcact cggaccatct ttcggcgtcg caccttccgg
     7441 tacgtggacg tggatgtcac gtttttcgta aaaatcaggg ttgatcccca gtttttccgc
     7501 acgcgcacga accaccgtta acgccgcctg aatagactcc tgcatcactt cgccgagcga
     7561 accggtatag gtcagtttgc ctttccccgg aacgcacgcg gtttcaatgg tcagcaagtc
     7621 accgcccact tccgtccacg ccagaccggt tacctggccg acacggtttt cgttatccgc
     7681 gcgcccatag tcgaaacgct gaacaccgag gtagtcatgc aggttatcac cgttaatttc
     7741 gatatgtttt aatgacttat cgagcagtaa ctgcttaacc gctttgcgac acagtttgga
     7801 gatttcacgc tccagaccac gcacgcccgc ctcacgggtg tagtaacgaa taatgccgat
     7861 aatggcgcta tcgtcgacgg tcagctcacc ttttttcaat gcattacgtt caatctgctt
     7921 cggcagcagg tgacgtttgg cgatgttcag tttttcatct tcggtataac cggagaggcg
     7981 aatcacttcc atacgatcca gcaacggtgc cggaatgttc atggagttcg acgtagcgac
     8041 aaacattacg tcgctgagat cgtaatccac ttccaggtag tggtcgctga acgctacgtt
     8101 ctgctctgga tccagcactt caagcagtgc ggaggccgga tcaccacgca tgtcagaaga
     8161 cattttgtcg atctcatcga gcaggaacag cgggtttttc acgcccactt tcgccatttt
     8221 ctggatcagt ttacccggca tagaaccgat gtaagtacgg cggtgaccac ggatttccgc
     8281 ttcatcacgc acgccgccca gcgccatacg gacatattta cgcccggtgg ctttggcgat
     8341 ggactgaccc agggacgttt tacctacccc cggcggccct accaggcaga ggatcggacc
     8401 cttgattttg ttgacacggc tttgaaccgc aagatactca aggatacgat ctttcacgcg
     8461 ctccagacca taatggtcgg tatcaaggat ttcctgcgcc tgacgcaggt cttttttgac
     8521 cttgctacgc gcattccacg gcacctgtac catccagtcg atatatccac gcactacggt
     8581 cgcttctgcc gacatcggag acatcatttt cagcttctgc agctctgctt ccgctttctc
     8641 ttttgcctct ttcggcatct tcgccgcgtc gattttgcgc ttcagggctt cgttttcgtc
     8701 cggcgcgtcg tccatttcac cgagttcttt ctgaatagct ttcatttgct cgttcagata
     8761 gtactcacgc tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc gtttctcaac
     8821 ctgcagcaga tcgatttccg attccatcat tgccatcaga tattccagac gttcgttaac
     8881 gtcggacatc tccagaacgg actgtttgtc agccagtttc agcggcatat gtgcagcaat
     8941 ggtatccgcc agacgcgccg gatcgtcgat gctattcagc gacgtcagca cttctggtgg
     9001 gatttttttg tttagcttga tgtagccttc gaactggctg attgcagtac gcaccagcac
     9061 ttcctgttcc cgctcatcaa tggtcggcga ctccagatac tccgccttcg cagaaaagtg
     9121 ttcgccattg tcagagagcg cagaaatacg cgcgcgctgt aacccctcga ccagcacttt
     9181 gacggtgccg tcaggcagtt tcagcatctg caatatagag gccacggtcc cgacggtgaa
     9241 aagatcgttt acacccggct catccgttga agcttctttc tgcgcgacca gcataatttt
     9301 tttatcatgg tccatcgccg cttccagaca acggatggat ttttcccgcc cgacaaataa
     9361 ggggatgacc atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg ggatttcaat
     9421 gcgttcagaa cgctcaggat tcatagagct ctctcttagt ttaatttccg ccaggtaatc
     9481 agatgacacg actgtgcttc acgccatcta ttaacatgta cgtcagtata tggggatgtt
     9541 tcccccacat tcaacgccga gaatagagga aaaattaaag gggagataaa atcccccctt
     9601 ttcggttaac taattgtatg ggaatggtta attattcacc agatgcctgt tgcgcttccg
     9661 gcttaccata aatcagcaac ggtttgcttt gaccatcaat taccgactcg tcgataacca
     9721 ctttttcgac atcttccatg gacggcagat cgtacatggt atcgagcagt gcggcttcta
     9781 cgatggaacg caggccacgg gcaccggttt tacgcgccat cgctttctta gcgatagcat
     9841 ccagcgcctc gtcacggaat tccagatcca cgccttccag attaaacagc gcctgatact
     9901 gcttggtcag ggcgtttttc ggctctttga ggatctgaat cagagcttct tcgctcagtt
     9961 cattcaacgt tgcgacaacc ggcagacgac caatgaactc agggatcaga ccaaacttga
    10021 tcagatcttc cggttcaacc tgcgccagca gttcaccttc gctggctttg tcggacttgg
    10081 ctttcaccgt cgcgccaaaa ccaatgccgg agccggtttc aacacgattg gcaatcactt
    10141 tatccaggcc agcaaatgcg ccgccacaga tgaacaggat cttagaggta tcaacctgca
    10201 agaattcctg ctgcggatgt ttacgcccac cttgcggcgg aacagcagct accgtacctt
    10261 cgatcagttt taacagtgcc tgctgtacgc cttcaccgga aacgtctcgg gtaatggacg
    10321 ggttgtctga cttacgagaa atcttgtcga tttcatcgat gtagacaatc ccgcgctgcg
    10381 ctttctgtac gtcgtaatcg cacttctgca acagcttctg aatgatgttt tcaacgtctt
    10441 cgcccacata accggcttcg gtcagcgtgg ttgcgtcagc catggtgaac ggaacgtcca
    10501 gcaggcgcgc cagcgtttcg gccagcagcg ttttaccgga accggtcgga ccgatcagca
    10561 gaatgttact tttgcccaac tcgacgccat tgctggtatc tccgttgcgc agacgtttgt
    10621 agtggttgta taccgcgacc gccagcactt ttttcgcctg ttcctgaccg ataacgtaat
    10681 cgtccaggtg gttgcgaatt tcatgcggcg tcggtagcgc actgcgttca cgatgcggtg
    10741 caacttcttt aatctcttcg cgaatgatgt cgttacataa atcaacacat tcgtcgcaga
    10801 tatacacgga tggaccggca atcagcttgc gcacttcatg ctggcttttg ccgcaaaaag
    10861 agcaatacag caatttgcct gagccatctt tgcgtttatc tgtcatgggt caaaacctct
    10921 tctttgttct ttgtgccgca cacgacgacg caaatgccat tctcaggcgc aagccgctta
    10981 cagcgttgtg ccgccctgga taagtatagc ggcacagttg cgcctctggc atcaattacg
    11041 atgggtcaga atcgaatcga ccagaccgta ttccaccgct tcaggggcag aaaggaagcg
    11101 atcgcgctcg gtatcacgtt caatctgttc taatgattga cccgtatgaa gcgccataag
    11161 ttcattcatg cgccctttaa ctttcagaat ttcacgggca tgaatttcga tatcggtcgc
    11221 ctggccctgg tagccgccca acggctggtg aatcatcacg cgcgaattcg gcaggcagaa
    11281 acgtttacct tttgcccctg cagtcagcaa gaaagcgccc atcgaggccg cctggcccat
    11341 acagatggtg ctgacatcag gcttgataaa ctgcatggtg tcatagatag acatcccggc
    11401 agtaatcacc ccgcctggag agttaatgta cagatagata tctttttctg ggttttctgc
    11461 ttccaggaac agcatctgcg ccacaatcag gttagccatg tggtcttcaa cctggccagt
    11521 cagaaaaatg acgcgttcct taagtagacg agaatagata tcaaaagagc gctcacctcg
    11581 tgaggtctgt tcaatgacca tcggcaccag cgccatatgg ggtgcaaagt tatctcgttc
    11641 gccgctgtat gacatttccg tctcctggat aaaattgaaa aaacctgctg taccgattgt
    11701 aaccttatgg acggattatc agctagtccc tgtgttatgg gtacggcatc accatatttc
    11761 aagcataaca atcttttgtt gttacgctaa caccgaaaga ggcttttcgc acggttccat
    11821 gcaaaattca tgacaaaaaa aagcccacca cctggcggtg acgggccttt gtgcgaattt
    11881 agcgcgttat gctacgtaaa ttacgcctgc tggttcatca gctcgttgaa agtggtttct
    11941 ttttcagtca ctttcgcttt cgccagtaca gcttcaacag cctgttcttc cagagcaaca
    12001 ttgcgcatgt tgtccatcag ttctttgttt ttgctgtaga actcgataac ttctttcgga
    12061 tcttcgtacg cagaagccat ctcttcgatc aagcctttca cgcgctcttc gtcagctttc
    12121 agctcgttgg tgcggataac ttcgcccagc agcaggccaa caactacgcg gcgtttagcc
    12181 tgttcttcga acagttcgcg cggcagttcc agagcttgtt tttcgttgcc accgaaacgc
    12241 tgtgcagcct ggcgacgcag aacgtcgatt tcgctgtcga tcagcgcagc cggtacatcg
    12301 atgtcgttag cttttaccag accttcgatc gcctgagact taacgcggtt acggatggcg
    12361 ctcttcagct cgcgctccat gtttttacgc acttcagcgc gcagaccttc tacggaacca
    12421 tcttcaacgc cgaaacgttt gatgaattct tcagtcagtt ccggcagttc acgctcttca
    12481 actttcttca ggttgatagc gaatttcgct gctttacctt tcaggttttc tgcgtggtac
    12541 tcttccggga aggtcacgtc gatggtgaac tcttcgccag ctttgtggcc tttgataccg
    12601 tcttcaaagc ccgggatcat gcgaccctga cccatcgcca gtacgaaatc agacgcttta
    12661 ccgccttcga actcttctcc gtctacagaa ccggtgaagt cgatggtcac acggtcttct
    12721 gcttcaacag cgccgtcttt ttctttccag gttgcctgct gtttacgcag agtatccagc
    12781 atgccgtcaa cgtcagcgtc ggtcacttca acaatcggtt tttcaacttc gatcgcttcc
    12841 agaccctgca gttcaacttc cggataaact tcaaactcta cagagtaggt gaagtcttca
    12901 cccagcttgt attcgcccgg aacataggtc ggtgcgccag ccggattgat tttttcttta
    12961 atgatggcgt caatgaagtt acggctcatc aggtcgccca ggacgtcctg gcgtacagac
    13021 gcgccataac gctgagcaac gatattcatt ggcactttgc ctttgcggaa gccgtcaata
    13081 cgtacttttt tcgcaacgtt gaccagctcg cttttaacag cggtctcgat gctgtcagca
    13141 gcgatagtaa tcgttacacg gcggccaagg ccttgagtgg tttcaactga aacttgcatc
    13201 ttgttacctc aaaaaatcac agtgctcggt caactcttac cccgcaagca caggttgttg
    13261 gcttcgcgga accgggatgt tcttataatc aatcacattc cctgttacca gaatcatccc
    13321 gaaagcattc aaataggacg cggcattata gcggcatcac ttttatgagt cgagaacggt
    13381 tattgcgcgc tgtcgcgtat tttttcacaa ttcccgggcg attttcgtct gaatgctggg
    13441 caaaaagcgc aaaatccaga caaaacaaaa cggcccgcag gccgtttttc ataaaatctt
    13501 tagcaacata ctggaaaagc gccgacagtt gcaaatgcgt ttttacgcga tgcttcctgc
    13561 tccacgacag ggaggagagg caaagacggt gtcctgcaac ccttcccact ccttaatagt
    13621 gtaagtatgc agagccagcg catgaacggt agtagagagt tcctccgcta aagtactgta
    13681 aatcattcga tgacgattca gaaaacgttc acccgtaaaa cgatcgctga ccagcacaac
    13741 tttaaaatgg ctttcagagc cggctgggac attgtgacga tagctttcat ccactacttc
    13801 gaggaatacg ggttggaacg ccgcccttaa tttttcttct atccgctcac gtatcatcat
    13861 gaaatatctc ctccgacaac gctgagatgt catccatccc tttaaatact agccgctttt
    13921 accgtttcca ttgcagctta acaacaaata tttaggtttc gtcctggttt ttccgtcaag
    13981 cgtatgaagt ttactgattt aaagattgaa acacaggcct cgcatcagcg tttcttttac
    14041 caggggtgat tatttcgtca acatgatgaa tttacatgcc ttacccactt cccctcgccg
    14101 ttggcaatgt tatgatggcg ggaattttta ccaaacacgc ttaagagtct ctgagagccc
    14161 gaacatgttc aaaaaaatcc tcttcccgtt agttgctctg tttatgcttg caggatgcgc
    14221 aaaaccgcca acaactatcg aagtttcccc gacgattaca ctgccacagc aggatccaag
    14281 cctgatgggc gtcaccgtaa gcattaatgg tgccgatcag cgtaccgatc aggcgctggc
    14341 aaaagtcacc cgcgataatc aaatcgttac cctgaccgcc tcccgcgatc tgcgtttcct
    14401 gctgcaagaa gtgctggaaa aacaaatgac cgcccgtgga tacatggttg gtccgaatgg
    14461 cccggttaat ctgcaaatca tcgttagcca actgtatgct gacgtgtccc agggcaacgt
    14521 gcgctacaac atcgcgacca aagcggatat cgccatcatc gctaccgcgc agaatggcaa
    14581 caaaatgacc aaaaactatc gtgccagcta caacgttgaa ggtgcgttcc aggcctccaa
    14641 caaaaatatc gccgatgcgg ttaacagtgt gctgaccgat accatcgctg atatgtctca
    14701 ggacaccagc atccacgaat tcatcaagca gaacgcgcgt taatttctgc cctctggccc
    14761 ggtgcaagcc gggcctgtag acgcccatgt ccagtcaata tttacgtatt tttcaacagc
    14821 cgcgttcagc catattgctg atcctgggtt ttgcttccgg gctaccgctc gccctgacgt
    14881 ccggcacctt acaggcctgg atgacggtcg agaatatcga tctcaaaacc attggtttct
    14941 tctctctggt aggccaggct tacgttttta aattcctctg gtcaccactg atggaccgct
    15001 acacgccccc atttttcggg cggcggcgcg gttggctgct cgccacgcaa attctgttat
    15061 tagtcgccat tgcggcgatg ggttttctcg aaccaggcac ccagctccgc tggatggcgg
    15121 cactggcggt ggtgatcgct ttttgctctg cctcccagga tattgtcttc gatgcgtgga
    15181 aaaccgatgt gctcccggca gaagaacgtg gtgcgggcgc ggcaatcagc gtgctgggtt
    15241 accgtttagg gatgctggtt tccggcggct tagccctgtg gctggcagat aaatggctgg
    15301 gctggcaggg catgtactgg ttgatggcgg cgctgttgat cccctgtatt atcgcgacgt
    15361 tgcttgcacc agaacccacc gacaccattc ctgtgcccaa aacgctggaa caagcggttg
    15421 ttgcacctct gcgagatttc tttggtcgca ataatgcctg gcttattttg cttcttatcg
    15481 tgctgtataa gctgggcgac gcattcgcta tgagcctgac aaccacgttt ttgattcgcg
    15541 gcgtcgggtt tgatgcgggt gaagtaggcg tggttaacaa aacgcttggc ttactggcga
    15601 ccattgttgg cgcattgtac ggtgggattt tgatgcagcg cctgtcgctg ttccgtgcac
    15661 tgctgatttt cggcatttta caaggtgcat ctaacgccgg ttactggctg ctgtcgatta
    15721 ctgataagca tctctacagc atgggtgcag ccgtcttttt cgaaaacctc tgtggcggga
    15781 tgggtacatc agcctttgtc gcgctgttaa tgacgctgtg taataagtca ttttccgcta
    15841 ctcaatttgc cctgctctca gcgctttctg ctgtagggcg agtttatgtc ggccccgtgg
    15901 cgggttggtt tgttgaagca cacggctggt cgacattcta tctattctcc gtcgccgctg
    15961 ccgtaccagg gcttattttg ctgctggttt gccgccagac gcttgaatat acacgagtaa
    16021 atgacaactt tatctcccgt accgaatatc ctgcaggtta tgcctttgcc atgtggacac
    16081 tggcggcggg catcagcctg ttggccgtgt ggttactgct gttgacgatg gacgcgctgg
    16141 atttgacgca cttctctttc ctgcctgcgc tgctggaagt cggggtttta gtcgccctct
    16201 ctggcgtcgt gcttggtggt ttgctggatt atctggcgct acgaaaaaca catctgacgt
    16261 aatctgtaaa tattatttag ctgtagtaat cactcgccgt aattattacg gctaaataaa
    16321 catcagcatc gcttattgat atttgttctt ttgtgcggct tagcgtctgg aaattattga
    16381 cgccatttat gaattctatt ttttttacac gattcagctg atgagtcctt attttctcac
    16441 cacccagttg tcaccctaat gatagttatt tgttaaataa ttgttttatt ttatattgat
    16501 tataccaatt gcccgcccaa actccttctg cacttccctt tcgttataac gcccttttgc
    16561 aacagcttct taaaatcaac ttgatatgtt ttgcaacata tgtgacctga tagccaaatc
    16621 caagtaacag gaatttaatc atgtttacag taatgtaacc ttcccgtaaa atgcccacac
    16681 actttaaacg ccaccagatc ccgtggaatt gaggtcgtta aatgagactc aggaaataca
    16741 ataaaagttt gggatggttg tcattatttg caggcactgt attgctcagt ggctgtaatt
    16801 ctgcgctgtt agatcccaaa ggacagattg gtctggagca acgttcactg atactgacgg
    16861 catttggcct gatgttgatt gtcgttattc ccgcaatctt gatggctgtt ggtttcgcct
    16921 ggaagtaccg tgcgagcaat aaagatgcta agtacagccc gaactggtca cactccaata
    16981 aagtggaagc tgtggtctgg acggtaccta tcttaatcat catcttcctt gcggtactga
    17041 cctggaaaac cactcacgct cttgagccta gcaagccgct ggcacacgac gagaagccca
    17101 ttaccatcga agtggtttcc atggactgga aatggttctt catctacccg gaacagggca
    17161 ttgcaaccgt gaatgaaatc gctttcccgg cgaacactcc ggtgtacttc aaagtgacct
    17221 ccaactccgt gatgaactcc ttcttcattc cgcgtctggg tagccagatt tatgccatgg
    17281 ccggtatgca gactcgcctg catctgatcg ccaacgaacc cggtacttat gacggtatct
    17341 ccgccagcta cagcggcccg ggcttctcag gcatgaagtt caaagctatt gcaacaccgg
    17401 atcgcgccgc attcgaccaa tgggtcgcaa aagcgaaaca gtcgccgaac agcatgtctg
    17461 acatggcagc gttcgaaaaa ctggccgcgc ctagcgaata caaccaggtg gaatatttct
    17521 ccaacgtgaa accagacttg tttgccgatg tgattaacaa gtttatggct cacggtaaga
    17581 gcatggacat gacccagcca gaaggtgagc acagcgcaca tgaaggtatg gaaggcatgg
    17641 acatgagcca cgcggaatcc gcccattaaa ggggttgagg aagaataaag atgttcggaa
    17701 aattatcact tgatgcagtc ccgttccatg aacctatcgt catggttacg atcgctggca
    17761 ttattttggg aggtctggcg ctcgttggcc tgatcactta cttcggtaag tggacctacc
    17821 tgtggaaaga gtggctgacc tccgttgacc ataaacgtct cggtatcatg tatatcatcg
    17881 tggcgattgt gatgttgctg cgtggttttg ctgacgccat tatgatgcgt agtcagcagg
    17941 cccttgcctc tgcgggcgaa gcgggcttcc tgccacctca ccactacgat cagatcttca
    18001 ccgcacacgg cgtgatcatg atcttcttcg tggcgatgcc tttcgttatc ggtctgatga
    18061 acctggtggt tccgctgcag atcggcgcgc gtgacgttgc gttcccgttc ctcaacaact
    18121 taagcttctg gtttaccgtt gttggtgtga ttctggttaa cgtttctctc ggcgtgggcg
    18181 aatttgcgca gaccggctgg ctggcctatc caccgctatc gggaatagag tacagtccgg
    18241 gagtcggtgt cgattactgg atatggagtc tccagctatc cggtataggt acgacgctta
    18301 ccggtatcaa cttcttcgtt accattctga agatgcgcgc accgggcatg accatgttca
    18361 agatgccagt atttacctgg gcatcactgt gcgcaaacgt actgattatt gcttccttcc
    18421 caattctgac ggttaccgtc gcgttgttga ccctggatcg ctatctgggc acccatttct
    18481 ttaccaacga tatgggtggc aacatgatga tgtatatcaa cctgatttgg gcctggggcc
    18541 acccggaagt ttacatcttg atcctgcctg tattcggtgt gttctccgaa attgcggcaa
    18601 ccttctcgcg taaacgtctg tttggttata cctcgctggt atgggcaacc gtctgtatca
    18661 ccgtgctgtc gttcatcgtc tggctgcacc acttctttac gatgggtgcg ggcgcgaacg
    18721 taaacgcctt ctttggtatc accacgatga ttatcgccat ccctaccggg gtgaagatct
    18781 tcaactggct gttcaccatg tatcagggcc gcatcgtgtt ccattctgcg atgctgtgga
    18841 ccatcggttt tatcgttacc ttctcggtag gcggtatgac tggcgtgctg ctggcagtac
    18901 cgggcgcgga cttcgttctg cataacagcc tgttcctgat tgcgcacttc cataacgtga
    18961 tcatcggcgg cgtggtcttc ggctgcttcg cagggatgac ctactggtgg cctaaagcgt
    19021 tcggtttcaa actgaacgaa acctggggta aacgcgcgtt ctggttctgg atcatcggct
    19081 tcttcgttgc ctttatgcca ctgtatgcgc tgggcttcat gggcatgacc cgtcgtttga
    19141 gccagcagat tgacccgcag ttccacacca tgctgatgat tgcagccagc ggtgcggtac
    19201 tgattgcgct gggtattctc tgcctcgtta ttcagatata cgtttctatt cgtgaccgcg
    19261 accagaaccg tgacctgact ggcgacccgt ggggtggccg tacgctggag tgggcaacct
    19321 cttccccgcc tccgttctat aactttgccg ttgtgccgca cgttcacgaa cgtgatgcat
    19381 tctgggaaat gaaagagaaa ggcgaagcgt acaaaaagcc tgaccactat gaagaaattc
    19441 atatgccgaa aaacagcggt gccggtatcg tcattgcggc tttctccacc atcttcggtt
    19501 tcgccatgat ctggcatatc tggtggctag cgattgttgg cttcgcaggc atgatcatca
    19561 cctggatcgt gaaaagcttc gacgaggacg tggattacta cgtgccggtg gcagaaatcg
    19621 aaaaactgga aaaccagcat ttcgatgaga ttactaaggc agggctgaaa aatggcaact
    19681 gatactttga cgcacgcgac tgcccacgcg cacgaacacg ggcaccacga tgcaggcgga
    19741 accaaaattt tcggattttg gatctacctg atgagcgact gcattctgtt ctctatcttg
    19801 tttgctacct atgccgttct ggtgaacggc accgcaggcg gcccgacagg taaggacatt
    19861 ttcgaactgc cgttcgttct ggttgaaact ttcttgctgt tgttcagctc catcacctat
    19921 ggcatggcgg ctatcgccat gtacaaaaac aacaaaagcc aggtgatctc ctggctggcg
    19981 ttgacatggt tgtttggtgc cggatttatc gggatggaaa tctatgaatt ccatcacctg
    20041 attgttaacg gcatgggtcc ggatcgcagc ggcttcctgt cagcattctt tgcgctggtc
    20101 ggcacgcacg gtctgcacgt cacttccggt cttatctgga tggcggtgct gatggtgcaa
    20161 atcgcccgtc gcggcctgac cagcactaac cgtacccgca tcatgtgcct gagcctgttc
    20221 tggcacttcc tggatgtggt ttggatctgt gtgttcactg ttgtttatct gatgggggcg
    20281 atgtaatgag tcattctaac gcaagcagcg gcgcatccca tggcagcgta aagacctaca
    20341 tgacaggctt tatcctgtcg ataatcctga cggtaattcc gttctggatg gtgatgacag
    20401 gggctgcctc tccggccgta attctgggaa caatcctggc aatggcagtg gtacaaattc
    20461 tggtgcatct ggtgtgcttc ctgcacatga ataccaaatc agatgaaggc tggaatatga
    20521 cggcgtttgt cttcaccgtg ctaatcatcg ccatcctggt tgtgggctcc atctggatta
    20581 tgtggaacct caactacaac atgatgatgc actaagagcg gcggttatga tatttaagca
    20641 atacctgcaa gtaacgaaac caggcatcat ctttggcaac ctgatctcgg tgattggggg
    20701 attcttgctg gcctcaaagg gcagcattga ttatcccctg tttatctaca cgctggttgg
    20761 ggtgtcactg gttgtggcgt cgggttgtgt gtttaacaac tacatcgaca gggatatcga
    20821 cagaaagatg gaaaggacga agaatcgggt gctggttaaa ggcctgatct ctcctgctgt
    20881 ctcgctggtg tacgccacct tgctgggtat tgctggcttt atgctgctgt ggtttggcgc
    20941 gaatccgctg gcctgctggc tgggggtgat gggctttgtg gtttatgtcg gcgtttatag
    21001 cctgtacatg aaacgtcatt ctgtctacgg cacattgatt ggttcgctct ccggcgctgc
    21061 gcctccggtg atcggttact gtgcggtaac cggcgagttc gatagcggcg cagcgatcct
    21121 gttggctatc ttcagcctgt ggcagatgcc tcactcttat gccatagcca ttttccgctt
    21181 taaggattat caggcggcaa acattccggt actgccggtg gtgaaaggca tttcggtggc
    21241 gaagaatcac atcacgctgt atatcatcgc ctttgccgtt gccacgctga tgctctctct
    21301 tggcggctac gctgggtata aatatctggt ggtcgccgct gcggtgagcg tctggtggtt
    21361 aggcatggct ctgcgcggct ataaagttgc tgatgacaga atctgggcgc gcaagctgtt
    21421 cggtttctct atcatcgcta tcactgccct ctcggtgatg atgtccgttg attttatggt
    21481 gccggactcg catacgctgc tggctgctgt gtggtaacac aacctctcag ttaaaaaggt
    21541 gctacggcac cttttttctt agcattagaa tcatatccct ctcgaaatat ttactaaaaa
    21601 atccgcatgt ttaccccatt cgtttgccgc tttacactag tcgcgaattt aaaacagagg
    21661 tggtaatgaa cgattataaa atgacgccag gtgagcggcg cgcgacctgg ggtttaggga
    21721 ccgtattttc attacgcatg ctgggcatgt tcatggttct gccggttctg accacgtacg
    21781 gcatggctct gcaaggtgcc agcgaagcat taatcggtat tgccattggt atttatggtc
    21841 tgactcaggc cgtttttcag attccgtttg gcctgctttc agaccgcatt ggtcgcaaac
    21901 cattaattgt cggtgggctg gcggtgtttg ccgccggtag cgttatcgct gcgctctctg
    21961 actccatctg gggaattatt ctgggccggg cgctacaagg ctccggtgcg attgccgcag
    22021 ccgttatggc gctgctttcc gatctcacgc gcgaacaaaa ccgcaccaaa gcgatggcgt
    22081 ttatcggcgt gagctttggc attacctttg ccattgcgat ggtgcttggc ccgatcatca
    22141 ctcacaaact tgggctgcac gtgctgttct ggatgatcgc tattctggca acaaccggca
    22201 ttgcgttgac catttgggtt gtgcccaaca gtagcactca tgtacttaat cgtgagtccg
    22261 gaatggtaaa aggcagtttc agtaaagtgc tggcggaacc gcggctgctg aaactcaact
    22321 ttggcattat gtgtctgcat attttgctga tgtcgacctt tgttgccctg cccggacaga
    22381 tggctgatgc ggggttcccg gcggctgaac actggaaggt ctacctggca acgatgttaa
    22441 tcgcctttgg ctcggtcgtg cctttcatta tctacgctga agttaagcgc aaaatgaagc
    22501 aagtctttgt cttctgcgtc gggttgatcg tggttgcgga aattgtgttg tggaacgcac
    22561 aaacgcagtt ctggcaactg gtggtcggcg tgcagctttt ctttgtggcg tttaatttga
    22621 tggaagccct tctgccttca cttatcagta aagaatcgcc agcaggttac aaaggtacgg
    22681 cgatgggtgt ttactccacc agccagtttc ttggcgtggc gattggcggt tcgctgggcg
    22741 gctggattga cggcatgttt gacggtcagg gagtatttct cgctggcgca atgctggccg
    22801 cagtgtggct ggcagtcgcc agtaccatga aagaaccgcc gtatgtcagc agcttgcgca
    22861 ttgaaatccc ggcgaacatt gccgcaaacg aggcgttaaa agtgcgtttg ctggaaactg
    22921 aaggcatcaa agaagtgttg attgcagaag aagaacattc agcttatgtg aaaatcgaca
    22981 gcaaagtgac gaatcgcttt gaggttgaac aggcaattcg ccaggcataa aagcgaggcg
    23041 aaattgcctg ctgcgctacg cttatcaggc ctactaagtt cctgagttac aaacctttgt
    23101 aggccgaatc aggcgttcat gccgcatctg acatgaacaa agcgcaggtt gtcgcgatta
    23161 atcgcggaag tttttgaact ggaacggctg acccagatcg ccaccacgta ccatcgccat
    23221 gacagcctgt aaatcatcac gagatttgcc cgtcacacgg atctcatcgc cctgaatttg
    23281 cgcctgcact ttcagtttgc tgtctttgat cattttgacg attttcttct gagtcgcgct
    23341 ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat gaacgatatt
    23401 ttccggtaca tccagtgaac tgccttcaat gccgcgcttc agcaacttgg cacgcagaat
    23461 atccagcaac tgattgacct ggaagtcgga ctcgctcaac actttgatgg ttttgctggc
    23521 gtcattcagc tcaaatgagg cttcaacgtt acggaagtca aaacgggact ccacttcgcg
    23581 gctcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga caatatcgaa
    23641 agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct gcaaagttaa
    23701 ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc aacagcatgc
    23761 acaattaagg gatagcctta ttgaacaccc ggagtggttg cgggtgagga ggaacaatga
    23821 aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca ctttgtaaac
    23881 agggtcacga cgttcagggc tggctgcgcg taccgcaacc ttattgtagt gtgaatctgg
    23941 ttgagacaga tggttcgata tttaatgaat cgctgaccgc caacgatccc gattttctcg
    24001 ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat gccgtcaaaa
    24061 gcctcgcgtc cacactgcct gtaaccacgc caatactgtt aattcacaac ggcatgggca
    24121 ccatcgaaga gttgcaaaac attcagcagc cattactgat gggcaccacc acccatgccg
    24181 cccgccgcga cggcaatgtc attattcatg tggcaaacgg tatcacgcat attggtccgg
    24241 cacggcaaca ggacggcgat tacagttatc tggcggatat tttgcaaacc gtattgcctg
    24301 acgtcgcgtg gcataacaat attcgcgccg agctgtggcg caagctggca gtcaactgtg
    24361 tgattaatcc actgaccgcc atctggaatt gcccgaatgg tgaattacgt catcatccgc
    24421 aagaaattat gcagatatgc gaagaagtcg cggcagtgat cgaacgcgaa gggcatcata
    24481 cttcagcaga agatttgcgt gattacgtga tgcaggtgat tgatgcaaca gcggaaaata
    24541 tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac tatatcaatg
    24601 gttttctctt acgccgcgcc cgcgcgcatg ggattgccgt accggaaaac acccgcctgt
    24661 ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt ttgcctcgcc
    24721 cctggtagtg aagagactga agccgtcacc actatcgacc tcttggttcg cggcggtatc
    24781 aaagtcacca ctgccagcgt cgccagcgat ggtaacctgg cgattacctg ctcgcgcggc
    24841 gtgaagctgc tggcggatgc gccgctggtc gaagtggctg atggcgaata tgacgtgatt
    24901 gtgctgcctg gtggcatcaa aggcgcagag tgttttcgcg atagcaccct gctggttgaa
    24961 accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc cgcgccagcc
    25021 accgtgctgg tcccgcacga tatcttcccg attggcaata tgaccggctt cccaacgctg
    25081 aaagacaaaa ttcccgccga acaatggcag gacaagcgcg tcgtctggga tgcacgggta
    25141 aaattgctga ccagccaggg gccgggtaca gctatcgact ttggtctgaa aattatcgac
    25201 ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac aactggtgat ggcggcaggg
    25261 atttataatt attacgagta gtgtcggatg cggtaagcgt cgcatctgac cagatgcgac
    25321 gttacaacca catattacgg gcgatacacc ttcacattgt taaagccctg ctcgcgcaga
    25381 tagagcgcct gcaggcggct catcaccccg cgctcacacc acaacagcca gtttttgctc
    25441 tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac cacatcaatc
    25501 ccttcgactt tcagtggctt atcttcctgt tcatcgatag aacggatatc gaggatcacg
    25561 tcgttcgggc cgaagccatt gacggtttcc acttccacca cttcctgctc ggtctgctgg
    25621 gcgatttcgc ggatatcaac gttattcgct tcctccacca ctttatcgag aatgctgaag
    25681 tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt cgggcttttg
    25741 gaaatcacgc cgcaatattc cggcatcgtg cgggcaaagt cttcggtgcc aatctggcga
    25801 gccaggttga tgatgtgctc tttgtcgtaa gagatcagcg gacgcaggat cagcgtatcg
    25861 gagacgttat caatcaggcg caggttggtc agcgtctggc tggacacctg gccgagcgct
    25921 tcaccggtaa ccagcgcctg tacgccgtaa cgttctgcca ctttggacgc ggcacgcacc
    25981 atcatacgtt tgaggataac gcccatctga ccgtcgtcga ttttctcgag aatttcaccg
    26041 acgaccggct caaagttaat ggcgacaaaa cgcacgcggt gggagctacc aaaacggttc
    26101 cacagataat gcgccacctg acgaacacca atttcatgcg ccgcgccgcc gagattaaag
    26161 aagcagtaat gcacgcggca gccgcgacgc atcaacatat aactggaaac accggagtcg
    26221 aaaccaccgg aaatgagcga caacacatct tcctgggtgc cgatcgggaa accgccaata
    26281 ccttcgtagc ggcctttaat cagcagcaga cgatcgtctt ccacttccag atggacagtc
    26341 acttccggat tggtcagctt cacgcgcgcg gattcaatat gctgatttaa accgccgccg
    26401 acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cgcggcgctt cacgcgtacg
    26461 cagaaggttt tgccttccag ctgatcgcga tattgaacca acgctttctc gaaaatatcg
    26521 tgcatgtcgg taaacggcac gtcttcgact tcgagaatat ggtggatacc cggaatacgg
    26581 gtcagggcgt cgcgaatagc cagacgctgg ttttcatctt ttgcgcgaac ttcgatgtta
    26641 tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt acgaatgttc
    26701 ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt gatttccggg
    26761 aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt aagcgacttg
    26821 tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga agtataccac
    26881 taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta tttgccctga
    26941 gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat gccgaagaaa
    27001 aatgaggctc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat tgtaacccgt
    27061 ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg cggcgtgcag
    27121 ctggcacgtc aggggcaggc taaattacaa caagccgaac agcgcgtaca aattctgctg
    27181 tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta atggactttc
    27241 cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt tttatcgccc
    27301 cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca ttattaggtg
    27361 gtaagcgcct gcgacctttc ctggtttatg ccactggtca tatgttcggc gttagcacaa
    27421 acacactgga cgcacccgct gccgccgtag agtgtatcca cgcttactca ttaattcatg
    27481 atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc tgccatgtga
    27541 agtttggcga agcaaacgcg attctcgctg gtgacgcttt acaaacgctg gcgttctcga
    27601 ttctaagcga tgccgatatg ccggaagtgt cagatcgcga cagaatttcg atgatttctg
    27661 agttggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta gatttagacg
    27721 ctgaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg
    27781 cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa ggacgtcgtg
    27841 ctctgccagt actcgacaag tacgcagaga gcatcggcct tgccttccag gttcaggatg
    27901 acatcctgga tgtggtggga gatactgcaa cgttgggaaa acgccagggt gccgaccagc
    27961 aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg aagaaagccc
    28021 gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact gactgaacag tcattcgata
    28081 cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa acaataagta
    28141 ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca ctggtcgact
    28201 ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc gacgaactgc
    28261 gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc gggctgggca
    28321 cggtcgaact gaccgtggcg ctgcactatg tctacaatac cccgtttgac cagttaatct
    28381 gggatgtggg gcatcaggct tatccgcata aaattttgac cgggcgccgc gacaaaatcg
    28441 gcaccatccg tcagaaaggt ggcctgcacc cgttcccgtg gcgcggcgaa agcgaatatg
    28501 acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt attgcggttg
    28561 ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt cattggcgat ggcgcgatta
    28621 ctgctggcat ggcgtttgaa gcgatgaatc acgcgggcga tatccgtcct gatatgctgg
    28681 tggttctcaa cgacaatgaa atgtcgattt ccgaaaatgt tggcgcgctc aacaaccatc
    28741 tggcgcagct gctttccggt aagctttact cttcactgcg cgaaggcgga aaaaaagttt
    28801 tctctggcgt gccacccatt aaagagctgc tcaaacgtac cgaagaacat attaaaggca
    28861 tggtagtgcc tggcacgttg tttgaagagc tgggctttaa ctacatcggc ccggtggacg
    28921 gtcacgatgt gctggggctt atcaccacgc ttaagaacat gcgcgacctg aaaggcccgc
    28981 agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa aaagacccaa
    29041 tcaccttcca cgccgtgcct aaatttgatc cctccagcgg ttgtctgccg aaaagtagcg
    29101 gcggtttacc aagctattca aaaatctttg gcgactggtt gtgcgaaacc gcagcgaaag
    29161 ataacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg gtcgagtttt
    29221 cacgtaaatt cccggatcgc tactttgacg tggcaattgc cgagcaacac gcagtaacct
    29281 ttgctgcggg tctggcgatt ggaggctaca aacccattgt cgcgatctac tccaccttcc
    29341 tgcaacgcgc ctatgatcag gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt
    29401 tcgccatcga ccgtgcgggc attgttggcg ctgacggtca aacccaccag ggcgcttttg
    29461 atctctctta cctgcgctgc ataccggaaa tggtcattat gaccccgagc gatgaaaacg
    29521 aatgtcgcca gatgctctat accggctatc actataacga tggcccgtcc gcggtgcgct
    29581 acccgcgcgg caacgcagtt ggcgtggaac tgacgccact ggaaaaactg ccaattggca
    29641 aaggcattgt gaagcgtcgt ggtgagaaac tggcgatcct taactttggt acgctgatgc
    29701 cagaagcggc gaaagtcgct gaatcgctga acgctacgct ggtcgatatg cgtttcgtga
    29761 aaccgcttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg ctggttaccg
    29821 tagaagaaaa cgccattatg ggcggtgcag gcagcggcgt gaacgaagtg ttgatggccc
    29881 atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt ccacaaggaa
    29941 ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccac cggtatggaa gccaaaatca
    30001 aagcctggct ggcataatcc ctactccact cctgctatgc ttaagaaatt attcatggca
    30061 ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgcgtt tcccgacttt
    30121 gcctcggatg tatgaccttt ggcgagccag atcgcggtaa tcacgcatgg acactgccgg
    30181 aagagagcag ccgtcccatc attaaacgtg cgctggaagg cggcataaat ttctttgata
    30241 ccgccaatag ctattccgat ggcagcagcg aagagatcgt cggtcgcgca ctgcgggatt
    30301 tcgcccgtcg tgaagacgtg gtcgttgcga ccaaagtgtt ccatcgcgtt ggtgatttac
    30361 ctgaaggatt atcccgtgca caaattttgc gctctatcga cgacagcctg cgccgtctcg
    30421 gcatggatta tgtcgatatc ctgcaaattc atcgctggga ttacaacacg ccgatcgaag
    30481 agacgctgga agccctgaac gacgtggtaa aagtcgggaa agcgcgttat atcggcgcgt
    30541 catccatgca cgcttcgcag tttgctcaag cactggaact acaaaaacag cacggctggg
    30601 cgcagtttgt cagtatgcag gatcactaca atctgattta tcgtgaagaa gagcgcgaga
    30661 tgctgccgct gtgttatcag gaaggcgtgg cggtgattcc atggagcccg ctggcgcggg
    30721 gccgtctgac gcgtccttgg ggagaaacca ccgcacggct ggtgtcggat gaagtgggga
    30781 aaaatctcta ccaagaaagc gatgaaaatg acgcacaaat tgcagaacgc ttaacgggcg
    30841 tcagtgaaga acttggtgca acgcgagcac aagttgcgct ggcctggttg ttgagtaaac
    30901 cgggcattgc cgcaccgatt atcggaactt ctcgggagga acagcttgat gaactgctaa
    30961 acgcggtgga tatcaccttg aagccggaac aaatcgccga gctggaaacg ccgtataaac
    31021 cgcatccggt cgtaggattt aaataggtat acaggcctga tgagacgtga caagcgtcac
    31081 atcaggcatc ggtgcacaac tacgacagaa tacccagcgg ccagtgatgt ccaataaagt
    31141 acaggatgac tgcggagatc accccggcga caatatcgtc aatcatgatc cccatgccgc
    31201 catgtacatt gcgatcaaac cagcggatcg gccacggctt ccacatatcc agaatacgga
    31261 aaatcacaaa tccggcggta acccactgcc agtcattggt cggcagcgcc atgagcgtaa
    31321 tccacatacc aataaattcg tcccagacaa tactgccgtg atcgtgcacg cccatgtctt
    31381 tcgccgtttg atgacaaaga tagacgccga tacagatccc cagcatcacc accagcgaat
    31441 agagctgcca gggcaaaaaa gtcatcagat accagaaggg aatcgccgcc agcgacccca
    31501 tcgtgccagg aacgatcggg cttaatccac ttccgaatcc gacagcaagc agatgccacg
    31561 gattactcat tttcaggcga cttttcgcga catctttatg gcgtggcaaa atggtcatat
    31621 cctttccagt ctaacgtgac aggttcgccg tcacgaataa aacaaagccc ttcgatatcg
    31681 gcggtcattt gcccgataca ggtaaacggt acgcccaggt gtccgagagc cacatccagc
    31741 gcgccacggt tcagttccgg caccgtaaaa cacaactcgt aatcttcgcc gccagagagc
    31801 gcccagcgca gcgcctgttc cggttcaaca tggcgagaaa gcgcatcaga aaacggcagc
    31861 aatgccaggt caatacgtgc gccgcagtcg ctggctttca cgatatgccc gagatcggaa
    31921 atcagaccgt cagagagatc gatagctgaa tttgccagat cgcgcagtgc ctgcccctgt
    31981 aaaatacgtg gcgacggacg gagatgacgt ttgatcaagt agtccgcatc tttagcatcg
    32041 gcaacctgca aacggttttg caaaatcgcc agcccggcag cgctatcgcc cggtgtaccg
    32101 gtcacataga tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg tcccatcgga
    32161 acaaagccgt ggatacccaa cgtcattgat aatggcccac gcgtggtatc gccgccaata
    32221 agttgcatat cgtaataatt gagaagatca aacaagctgt cgctgaagga ctcaagccac
    32281 gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc ggcccccatc
    32341 gctgccagat cgcttaggtt caccgccagt gctttataag ccagatcagc aggatcgata
    32401 tcagggagga agtggttgcc cgccaccagc gtatcagtgc tgatcgccag ggtctgtttc
    32461 tcggggatat tgagaagagc gcaatcgtcg ccgatgccca gttcgacatc aagacgagaa
    32521 cttcttacac ggtcaaaata acgggcaatc agggagaact cgccacatgc catacgttac
    32581 gcctcagcag ataaaaaaga aaaggccggc gatcatggaa cggtcttccg tgaatctacc
    32641 ggcttggata ttactttttg ttagggcgaa tcacaggtgc tgctttatcg agtacgccgt
    32701 tgacgaactt atggctgtct tctgcgccga acgatttcgc cagttcgatc gcttcgttaa
    32761 tggccacttt gtatggcaca tcgctacgtt tagacagctc gtacagcgca atgcgcagta
    32821 ctgctttttc tacctggccc agttcttcca gcagacggga caggtacggc ttcatcagtc
    32881 cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag ctcacggaag tacaggacgt
    32941 caacgtcttt tacatcctgt tcagccagga actggtattc aacatcagcg atgtcgttct
    33001 gggacaactg ccaggagtag agcgcctgga cggcacactc acgagcgcgg cgacgagcag
    33061 caggtttcac ggatttcccc ttactaattt caggccttga tggctttcaa tacattaatc
    33121 atttcaagcg cggtcagtgc agcttctgca cctttgttgc cagctttggt gccagcacgt
    33181 tcgatcgctt gttcaatgct ttcagtggtc agaacaccaa aagcaaccgg aatttcgctg
    33241 tcctgggcaa catgcgccag gccgttgctt gcaccaccag cgacatattc aaagtgggca
    33301 gtgccaccac gaataaccgt acccagcgca atcaccgcgt cgtatttacc ggttttagcc
    33361 agtgcacccg ccgccagcgg cagctcatag gcaccaggca cccaaacaac ggtaatgttt
    33421 tcatctttta cctgaccgat acgtttcagt gcgtcaattg caccttccag caggctgtca
    33481 ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg ggtagcaacg
    33541 ttagcttcaa taatgttcat tttcttcctt cgggttcgag tatggccccg caggggggcg
    33601 gattttagca taatatttcg tgcgctgctt ccctttcgag ccgggagatc atgcacccac
    33661 taaatgcagg caaacatccg ggcctacatg acgtatctct ttgaatttaa attggggagc
    33721 gtcggctaat ttctcaagcc ctggcagcgt gcataatcca cgggcgtcgt tgcctaatag
    33781 tttaggtgcg atatagacaa tcagctcatc gactaaaccc gcctgtagca atgcgccagc
    33841 gagcgttggc cccgcttcca cccagatgct gttaatctgc tgtttaccca gttgcatcat
    33901 tagtacaacc agatccaggt gacctttatg ctctggaatc agcaaggtac gcaccgtttc
    33961 cggccactca cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg gctgctgcac
    34021 aatgcgatgt tccggcgtca cgcgattttg gctatcaatc acaatacgta ctggctgacg
    34081 gagattttgt tgcgggtaga gcccctgagt ttgttcatcc agttcagacc aacgcactgt
    34141 taaggctgga tcatccgcca gcaccgtggc gctgctggtt aaaatggcat gactttgcgc
    34201 gcgcagtcgt tgtacgtcgc gtcgcgcctg aggcgaagtg atccactggc tttcgccact
    34261 cgccatcgcc gtgcgaccat caagcgatgc gccaagtttt aactgaatat aaggaaagcc
    34321 ggtgcgcatc cgcttgagaa agcctttatt caattgctca gcttcactca tcatcagccc
    34381 gtggctgacg tcaatgccag cctgttgcag acggtaaagt ccacgtccag caacctgcgg
    34441 attggggtct tgcattgcag caaccacgcg cgccacgccc gcagcaatta atgcatcaca
    34501 acacggtggc gtacgaccat gatggctaca gggttcgagt gtgacatacg ccgttgcgcc
    34561 tttggctttt tcgccggcca tgcgcaacgc gtgtacctcg gcatgcggtt cacccgctcg
    34621 ttgatggtaa ccttcaccga caatttcgcc atctttgaca atgacgcacc cgacattcgg
    34681 gttgggatgc gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg ccatgtaata
    34741 ctcgtcctgc acggcttagt cctccaggcg cgcgatctct tcgccaaatt ctttgatatc
    34801 ttcgaaactg cggtagacag aggcaaaacg gatataggcg actttatcga gctttttcaa
    34861 ttgctccatc accagattgc caatcatctt gctcggcact tcgcgctcac cggtggcgcg
    34921 cagctgcgat tttatatgat tgattgccat ttcgacgtca tcggaactca ccggacgttt
    34981 ttccagcgcc cgcagcattc cgctacgtaa tttctcttca ttaaacggtt cacgcacgtc
    35041 gttgcttttt acaacacgcg gcataaccag ctccgccact tcaaaggtgg tgaaacgttc
    35101 attacacacc agacactgcc gacggcggcg tacggatgaa ccctcgccca cgagacgaga
    35161 gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc gtcctgacca
    35221 ggtggttaac agaaatctat tttaccctga actgacgata caacaaaggc gcaacgcttt
    35281 tgagataaag gatcgatgct ttcactgctt ttaccctcta ccattaggct aattgcgacg
    35341 agaaagggac gacctatgaa tactaacgtt tttcgactgc tcctgctggg aagcctgttc
    35401 agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag cgtcagcacg
    35461 ctgaaccagg agatgacgca actcaataaa gaaacggtca aaatcactca gcaaaacaag
    35521 ctgaatgcaa aatccagcag tggggtttac cttctgcctg gagcaaaaac accagcaaga
    35581 ctggaaagcc agatcggtac tttacgtatg tcgctggtga atattacgcc tgacacagat
    35641 ggcactaccc tgacgctacg tattcagggc gaatccaatg accctttacc cgcattcagc
    35701 ggaaccgttg aatatgggca aattcaggga acaatagaca actttcagga aatcaatgtg
    35761 cagaatcaat tgattaacgc ccctgccagc gtcctcgccc ccagcgatgt tgatattccg
    35821 ttacagttaa agggtatttc tgtggatcag ttaggctttg tacgtatcca cgacattcaa
    35881 cctgttatgc agtaaacgta ttgcggggcg atattgtgcg tcccgcaaca tctttctccg
    35941 tcattttgtt actccgctta catcacctgg attgatagta aaagtttgca acaagggtga
    36001 aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg
    36061 atagaactgt gaaacgaaac atatttttgt gagcaatgat ttttataata ggctcctctg
    36121 tatacgaaat atttagaaac gcaatttgcg cctttttcac tcccgcaagg gattttcaaa
    36181 cagtggcata catatgaaaa aaacattact ggcagccggt gcggtactgg cgctctcttc
    36241 gtcttttact gtcaacgcag ctgaaaacga caaaccgcag tatctttccg actggtggca
    36301 ccagagcgtt aacgttgtcg gaagctatca cacccgtttc ggaccgcagc tccgcaacga
    36361 tacctacctt gagtacgaag cattcgctaa aaaagactgg ttcgacttct atggttatgc
    36421 ggatgcgccg gtattcttcg gcggtaactc cgatgcaaaa ggtatctgga accacggttc
    36481 tccgctgttt atggaaatcg aaccgcgttt ctccattgac aagctgacca atactgacct
    36541 tagcttcggt ccgttcaaag agtggtactt cgcgaacaac tacatttacg acatgggtcg
    36601 taataaagat ggccgccaga gcacctggta catgggtctg ggtaccgaca tcgatactgg
    36661 tctgccgatg agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga actacggcgc
    36721 agcgaacgaa aacgagtggg acggttaccg tttcaaagtg aagtactttg tgccgattac
    36781 cgatctgtgg ggcggccagc tgagctacat cggcttcacc aacttcgact ggggttccga
    36841 cctgggcgat gacagcggtt atgcgaacaa cggtattaaa acacgtacca ataactctat
    36901 tgcgtccagc catattctgg cgctgaatta cgatcactgg cactactctg tcgttgcccg
    36961 ttactggcat aacggtggtc agtggaatga cgatgcagaa ctgaacttcg gcaacggcaa
    37021 cttcaacgtt cgctctaccg gctggggtgg ttatctggta gtaggttaca acttctaatt
    37081 gtgagaatgc cggatttcat atccggcatt ttttatcact tcttcttgtt catcatcgct
    37141 ttcaaatcag cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct
    37201 gcgataacgg tcgtaccata ctgatccgct tcggtgtatt tcgaatgctc atgatcgtgg
    37261 caatagagac aaagtaattc ccagttactg ccatcttccg ggttattagt atggtcgtga
    37321 tcaatgtggt gaacggtaag ttcacgcagg ttggaataaa caaactcgcg ggaacagcga
    37381 ccgcagaccc acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgcgca
    37441 tagttttttg ggatgatagc catgaaaaag ttacctggat gaaatgtagg gttacgcgca
    37501 gtttcccaag atgcggggat tttcgcaatc accttacatc aaaaaaaatc ccgatcttct
    37561 gaccgggatt ttcaacatca gctgaaactt gattaaggca gaattgacgg ctgatccgcc
    37621 ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc
    37681 agcgcagatg ccacatagat ggaagatgca gtaccgatgg aaacaccgat aagcatggtc
    37741 agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat aaccatcaag
    37801 gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt
    37861 tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaatacggtc cgataccacg
    37921 atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca
    37981 atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca
    38041 ataacaaccc ctgccgccag tcgccactca aagcggaaac caacgtacac gaggatagac
    38101 agcagcgccg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga
    38161 ccgacgaact caatacgctt cactgccgca ttctgattgg tggattcgtt aatcaccttc
    38221 agaacctggc tgcccagcac ctgaccaccg gtttcgcctt cagcaggcgg catacggacc
    38281 atgatgtcgt ggctgctacc aaagttttgc agcatcggct cttcaaaacc ggctttttgc
    38341 aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc aataaccgta
    38401 ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcggcg
    38461 attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact
    38521 ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat
    38581 tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac
    38641 aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat
    38701 tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc
    38761 gaagatggaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc
    38821 gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc
    38881 aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg gcaacagcga
    38941 cataataccg acaatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa
    39001 cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc
    39061 cagcccctgc tcaatgttct gcatacccag ggttgggcca atggtacgtt cttcaacaat
    39121 ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gaaagctgac gcgcttcgct
    39181 tgggttattg atgccagtaa tacggaagct gttacccaga cgagactgga tgttggcgat
    39241 attaatcacc tcttcctgtt tcaccagaac cgcacgaccg tttgcatctt tcttaccgct
    39301 gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt
    39361 agacatgatg ttaccacccg cgctatcgag agagatgtta acctgcggct ggttgtattc
    39421 gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc gtttgtacag
    39481 cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga
    39541 tgccgcagcg gcctgatcaa cgttggtgtt taccagacgg aattccagcg ttgccgtcgc
    39601 acccagaatc tctttcgcac gcgcagtgtc ctgaatacct ggcagttcaa caacgatacg
    39661 gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg
    39721 caggatattg atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct
    39781 cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt
    39841 gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc
    39901 gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcacgca ggtcgctgcg
    39961 caggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat
    40021 caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc
    40081 tgccagccag cgcggcgttg ccggggcaag attaagcgcc acgacgtatt tgtcacccat
    40141 aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca gtggagtcga agcgcgcaag
    40201 aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt
    40261 ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac
    40321 agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac
    40381 gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa
    40441 aaattttaat tacagcgcct tcatggtgcc tttcggcaga acggcagcta cgaagtcacg
    40501 tttaataact acttcagtgg tgtcattcag tgcgatagca atgtagccgt tttccgctac
    40561 tttggttacg cgaccgacca ggccaccgtt cgtcagcact tcatcacctt tagcgatgga
    40621 gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata
    40681 gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg
    40741 tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt
    40801 aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta
    40861 cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt
    40921 agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga
    40981 gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga
    41041 gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca tcggtcacga
    41101 acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc
    41161 gacgtacgcc ctcaaccagg tcttctggtt taccaacgcc catcaggtaa cgcggtttgt
    41221 ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct ttcggctcac
    41281 ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga cctttaacag
    41341 aaatatcacg taaatcttcg taaacactgc cctgaatgat accaaacagc gcatttttgt
    41401 ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatgg
    41461 agcgttttgc ataatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga
    41521 tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt
    41581 cgccgttgat cgggttacgg aagtgaacac cctgttcggt gattttacga atatcaccaa
    41641 ggctgaaaac ctggaagccg ccggagtcgg taaggattgg tcctttccac tgcataaaat
    41701 cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg
    41761 tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta
    41821 cggtgccgta agtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga
    41881 ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc
    41941 cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccaacgc
    42001 gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc
    42061 gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa ccagcaaatg
    42121 ccgaaaccag cataatcagc gtcgattccg gaaggtggaa gttcgtcacc agcgcatcga
    42181 ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcaa
    42241 tgagatcgtt tttcgctgcc tgagccgcac tttccagcga acgtactgag gtggtgccaa
    42301 ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg
    42361 gcacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct
    42421 ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta
    42481 atttttccag caaaggttcg tcaaagtgca gtccggcggt cggtgctgct accgcgccgg
    42541 gtttttcgct ataaaccgtt tgataaagtt cgcggtctgc atcttcgtcc ggacggtcga
    42601 tgtacggcgg cagcggcata tggccgatgc tgttgagaat atccagcacc gaacgttgat
    42661 cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttaatacttt
    42721 cgtcatcgcc cagcagcagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg
    42781 caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg
    42841 ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc
    42901 cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc
    42961 cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg
    43021 attcgggcaa ttcaaaagag aaatcggtaa cgcgcatgac actgactcag actaaaataa
    43081 gaggcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga
    43141 atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt
    43201 tccggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac
    43261 gtcgtggctg gcattcatat gcatcggcga atcgacgtat taaccgacaa tctgccagaa
    43321 gtccgcgaag cgcgggagtg gtttcgtaat gaaacgcgcc gtgtcgcgcc tattacgctg
    43381 gatgtgatgt gggatcactt tctgtcccgc cactggtcgc agctgtcgcc cgattttccg
    43441 ctacaggaat ttacctgtta tgcccgggag caagtgatga ctattcttcc agactcaccg
    43501 ccacgcttta tcaatctgaa caactactta tggtcagagc agtggttggt gcgctatcgc
    43561 gatatggatt tcatccagag cgtgttaaac ggcatggcaa gccgtcgccc acgtctggat
    43621 gccctgcgtg actcctggta cgatttagac gcccattacg atgctctcga aacccgcttc
    43681 tggcagtttt atccgcggat gatggagcag gcgtcacgca aggcgttata agacgttaat
    43741 tcatccacac cgtagccgaa atggcaggca aagccagaat cccgtccgtc agttgcccgt
    43801 gaccttcttt gcgttgccat tgcgcgacat taagcaacgg cgacgcgggt agcaccactt
    43861 cacaggcttc gccacggttg attgccacca gtacacgctg ctgattcagc acgcggacaa
    43921 ataccaccac gttatcttcc gcatacagca cctgacagcc gccacgacgc aacgcctgac
    43981 ttttcttacg cagcgcaatc attcgctggt acagcgcgaa taacgccgaa tcctgctttt
    44041 ccacctgcca ggggaacggc ttacggcaaa acggatcgtt tttgccatcc agtcccactt
    44101 catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccaggg
    44161 gcaggcgcgc aacatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga
    44221 gctgattaaa catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc
    44281 aggccataca ggtttgggcg tcaatttgct gcggatcgta agagatatcg gtgttggcaa
    44341 gaaatcccca caacgggaat gtgaagccgc gatagttcat ggcggcatct tccacatcgg
    44401 cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac aatatacgca tccggctggg
    44461 tttctttcgc cgcttcggtg atcccggcaa cgtgctgcaa attattgcgc gccccacccg
    44521 cttcccccag catatgcacc acatccagcc gccagccatc catattccac ggcgctttca
    44581 gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact
    44641 gataatccag cttcggcaag ctggcatagc ccagccagtc gagcgccgtg ccatcatcac
    44701 taaacgagta ccagtcacgc cagggtgatt cggggttgtg acaagctccc cccgtgccac
    44761 gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
    44821 ccagtcgtat tcccagctgc tgcgtattgt gtcgcaaacg cagcaacgcc ccatcaccgc
    44881 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgcacg ctgggagctt
    44941 taaacaccgg attgagatac agcgccgtca cgccaagttt tttcagatac ggcagttttt
    45001 cacttatccc gtccagatcg ccgccataga acgttgatcc gccagcctgc gccgtgaccg
    45061 gttcatccca gtcacgcaat atgatctctt gtccggctgc gtgatggtaa tagacatgat
    45121 cctgttcagc ttcacgagga agattacggg cgaaacgatc agggaagatc tgataaaaaa
    45181 tctgatccgc agcccattgt gggccgatat ccggtacatc gatggcaaac tgctcctgtc
    45241 gtgccggcgg cattcggctg aagccttgcg gtgtaaacca acgctggcga tcgtgccaca
    45301 gcaatttgaa actataacgc cgccgaggtt gtccgcagga gagctcgatc gccgcccgcc
    45361 atgcggtgac gccaggctgc ggctgactgc gctgcttatg catcggtact gacatttctt
    45421 cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
    45481 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc agatgccatg
    45541 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
    45601 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
    45661 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaaggatt actggttttc
    45721 agccagctga cgatcgtggc ggcgcttaaa catccagcca atcaacagca caacaatcca
    45781 cgcgaaacca acatacagcg agatacgcgt atcagggtga taaccaatca acccgataat
    45841 aaagagcagg aaaatcaaac cgccgatggt cgttgctacc ccacccggca ctttaaattt
    45901 cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcgatttgcg acagcaggat
    45961 cataatccac acccacaccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
    46021 ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac
    46081 cgtaacccac ggaataccgc gacgcgaggt tttgctgaaa atttttggcg cgctgccctg
    46141 ctctgccata ccgtggagca tacggcctac accaaatacg tcactgttaa ttgccgacag
    46201 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
    46261 gaacgtcagc acaaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
    46321 aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc
    46381 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
    46441 cccaccgtaa gcaaacatca ccatttgcaa cgacatcacc ataccaagcc agccgttact
    46501 gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaat
    46561 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatagtgg cgactttaaa
    46621 gaacgagaac cagaactcca gctcaccgaa taccttcacg ctcatcaggt ttacggcgca
    46681 aatgatcagc accacactca gtacccaaat ccagtgcggc accgtcggga accagacacc
    46741 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta
    46801 ggtccagccg gtaatgtaac ctgccagcgg gccgaggttt tcctgcgcat aacgcgagaa
    46861 agagctggct gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc
    46921 cgcgatacca ccgataatat aggccaacaa cacgcttgga ccggccattt tgatggcatc
    46981 tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
    47041 tcgggtactt agcccacgct ttagcttgtt cttactttcc atcaattcct aacccatcaa
    47101 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
    47161 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
    47221 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
    47281 ccaggacggt aagatatcgc tgaatgcaga tgccttgatc ccgtcgagaa taccaaaaag
    47341 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
    47401 aaaacttaat acaaccagtg cgatacacgg cggataaatg gcggtcagca ccggtacaga
    47461 gatctgaatc agctggctca agccgaggtt agacaccacc atcgagaagc cgccgaggat
    47521 aaacaccagc gtacgataag agagcggtac gtactgggcg aagaattctg cacaagcaca
    47581 ggtcaggcca accgccgtga ccaggcaggc gatgaagatt aacgccgcca gcaggaagct
    47641 accgccgccg ccaaaggtat gctgaacgta agcatgcagg atcgccgcac cgtttgcaga
    47701 ctgatcgacc agcgacgcgc tgtctgaacc cagacggaac agcgccaggt acagcagagt
    47761 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    47821 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    47881 cgtatccatg gtcagatagc cgttgacgaa gccgttagaa aacgcagcgt tttgataagc
    47941 ctcagtcgcc gtgctgatag aacccgccgg ccagataatt gcggcaacag acaggatgac
    48001 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    48061 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtaaataa acagcggcag
    48121 cgcggaatca cccgtcagcg gcgcaatgcc cacttcaaaa gaaacggtag ctgtacgcgg
    48181 cgtagcgaaa agcggcccca ccgccaggta acaaactgtt gccagcagta cgccagcgac
    48241 tttaccaatt ggcgtgctga gactgtcaac accgccgcca acttttgcca gcgccactac
    48301 cgttaatacc ggtaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    48361 ttcgcctgcc tgcaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    48421 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    48481 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    48541 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    48601 atagtttttc gatttaccgc gtaaatgcag tcagtatgac agcacctgaa gcgcaattta
    48661 aacgcttata acgttttaaa gcaagaaggg atctaaaaac cagaggtata tagcgataag
    48721 aaatcaccaa ccagtataaa acactatttc aatgaaaaag acatggctaa tcatgcgaac
    48781 aaaaaatcac caaccattgt aaaatctaac gcccgtattt cgactgcaaa gaaaataagc
    48841 cagcatcggc tggcttatgg caataaaaga tgacaaaggc gaattaatcg ctgtttttgg
    48901 caattaaacg ttccgggata ataaaactga aacgtgtacc ttttcctact gtactctcaa
    48961 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    49021 taccaccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    49081 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac actaaattcg gcaccgtgcg
    49141 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    49201 agaccaggtt cgaaatcgca ctacgtagct gatcttcgtt gcctgacacc ttgaggccgt
    49261 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    49321 cgacgcgcag catcatcggc acatcaacct tttcattgag caaatgcgtc ggtgcggctt
    49381 ctattttcga cagcgtcagc aattgcttca ccaacccttc catccgctgg gtctgctcac
    49441 gcatggtgtg caacgctttt tcacgtaccg cgccttccag cggttgctca tccatcatct
    49501 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    49561 agttacgccg cgccccttcc agttgatgca tttgcgtgac atctcgcgcc accatcagca
    49621 actgtttgtg ggtataaggc atgacgcgaa tctccagatg ccgcccggta ttgagcacca
    49681 gattgagcgg gcgagagaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    49741 gcaagttaag gatgttctgc ccgttatctt ccggccagcg caagccaaga atctgttgcg
    49801 ccagaccgtt gcaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca
    49861 gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt
    49921 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    49981 gtggcggggt catactacga tccacccaca gccaccatga aaggcgcaat aaattccaga
    50041 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taacccaaaa
    50101 atgcacccag gatgaaagcc gggaggcagc aaaatagcag ctccagcacc agccttttcc
    50161 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctacg
    50221 tccgatgagc aaggccttaa aagcgggttg aaaaacgata ccctgtaccg cgcacggtct
    50281 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    50341 cgaccgtacg atcttccaca tacacgttgg ttccccatac gtgatttaac agttgctcgc
    50401 gactgtacac gcgctcggga tgcgtcataa aaaagtgcag cagtttaaat tcagttggcc
    50461 ccatctccag cggttcttcg cccgccatca cccggtgaga tgtcggatcg agacttagcc
    50521 cctgcatctc aatcacctct tccaccgcca tcggcgatat acggcgcatt accgctttaa
    50581 ttcgcgccac cagctccttc ggagaaaacg gcttggtgat atagtcatcc gcaccggttt
    50641 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    50701 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    50761 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    50821 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    50881 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    50941 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    51001 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcacag aaaaaagcaa
    51061 atggcacatc tgtttgggta taatcgcgcc catgcttttt cgccagggaa ccgttatgcg
    51121 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    51181 tgaacatcag gcttttcttg actggctgct ggagacagca caagcccatc tggtggatgc
    51241 gattattgtt gccggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    51301 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    51361 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    51421 taccgtggtc gccagcgccg gacatgcgcc gcaaatattg ccccgtcgcg acgggacgcc
    51481 aggcgcagtg ctgtgtccca tacctttttt acgcccgcgt gacattatta ccagccaggc
    51541 ggggcttaac ggtattgaaa aacagcagca tttgctggcg gctattactg attattatca
    51601 acaacattat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    51661 tggacattta acgaccgtgg gtgccagtaa aagtgacgcc gtgcgtgaca tttatattgg
    51721 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    51781 tattcaccgc gcgcagctta ttggcggcat ggaacatgtt cgctattgcg gctcccccat
    51841 tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa
    51901 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta acgcaaccca tggcggtgct
    51961 gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcacagga
    52021 gccgcctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    52081 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gccggagtcg
    52141 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    52201 cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gatgaatcac agcagcaacg
    52261 tctgcagcat cttttcgcca cgacgttgca tagcctcgcc ggagaacacg aagcatgaaa
    52321 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    52381 acccgcgagc cgttcgccag caacgggctg tttgctatta ccggcccaac cggtgcgggg
    52441 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgcctctct
    52501 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgccgaatg tctggcggaa
    52561 gtggagtttg aagtcaaaga tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    52621 aaccagccgg acggtaattt gcaggtgcca cgcgtagagc tggcgcgctg tgcagacggc
    52681 aaaattctcg ccgacaaagt gaaagataag ctggaactga cggcgacgtt aaccggactg
    52741 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    52801 aatgccaaac ccaaagaacg cgcagaattg ctcgaggagt taaccggcac tgaaatctac
    52861 gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcacaga gctggagaag
    52921 ctgcaagcgc aggccagcgg cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca
    52981 gcgagtttgc aggtacttac tgacgaagaa aaacagttaa ttaccgcgca gcagcaagaa
    53041 caacaatcgc taaactggtt aacgcgtctg gacgaattgc agcaagaagc cagccgccgt
    53101 caacaggcct tgcaacaggc attagccgaa gaagaacaag cgcaacctca actggcggcg
    53161 ctcagtctgg cacaaccggc acgaaatctt cgtccgcact gggaacgcat cgcagaacac
    53221 agcacggcgc tggcgcatac tcgccagcag attgaagaag taaatactcg cttacagagc
    53281 acaatggcgc ttcgtgcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    53341 cagcagcaac aaagcctgaa tgcctggtta caggaacacg accgcttccg tcagtggaac
    53401 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg
    53461 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc
    53521 acgttgacgt taaccgccga tgaagttgct agcgcccagg cgcaacatgc tgagcaacgc
    53581 ccactgcgtc agcgcctggt cgcactgcat gggcagattg ttccccaaca aaaacgtctg
    53641 gcgcagttac aggtcgctat ccagaacgtc actctggagc agacgcaacg taatgccgca
    53701 cttaacaaaa tgcgccatcg ttataaagaa aagatgcaac aacttgccga tgtgaaaacc
    53761 atttgcgagc aggaagcgcg cattaaaacg ctggaagccc agcgcgcaca gttacaggcg
    53821 ggtcagcctt gcccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    53881 ctggaaccgg gcgttaatca ggcccggcta ttaacgctgg aaaaagaagt gaaaaagctc
    53941 ggcgaagaag gtgcgacgct acgtgggcaa ctggacgcct taacaaagca acttcagcgc
    54001 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    54061 gccgtcacgg ccagcctcaa tatcaccctg cagccacagg acgatattca accgtggctg
    54121 gatgcacaag atgagcacga acgccagctg cggttactca gccaacggca tgaattacaa
    54181 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgc
    54241 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    54301 gaagagagct ggttggcgac gcgtcagcaa gaagcgcaga gctggcagca acgccagaac
    54361 gaattaaccg cgctgcaaaa ccgtatgcag cagctgacgc cgattctgga aacgttgccg
    54421 caaagtgatg aactcccgca cagcgaagaa actgtggcgc tggaaaactg gcggcaggtt
    54481 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg
    54541 gcggcgcaaa gtctgcaaaa agcccaggcg cagtttgaca ccgcgctaca ggcgagcgtc
    54601 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg
    54661 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    54721 acagcagaaa cgctgacaca gcatcagcaa caccgacctg gcgggttgtc tctcactgtg
    54781 acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    54841 acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa
    54901 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    54961 aattcgctaa taggttccaa agagggcgat aaattccgca aatttgccca agggctgacg
    55021 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg
    55081 ttacagcgca aagccagcga ggcgctggaa gttgaggttg ttgatacctg gcaggctgat
    55141 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgtcag tctggcgctg
    55201 gcactggcgc tttcagatct ggtcagccat aaaacgcgta ttgactcgct gttccttgat
    55261 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gttggatgcc
    55321 ctgaatgcca gtggcaaaac catcggggtg attagccacg ttgaagcgat gaaagagcgt
    55381 attccggtgc agatcaaagt gaagaagatc aatggactgg gctacagcaa actggaaagt
    55441 gcgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct
    55501 tggcccgact ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    55561 gttctggtgg ttgtgatggt ggtatgaaaa aagttatttt atctttggct ctgggcacgt
    55621 ttggtttggg gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg
    55681 taggaatttc gattcctgcc gccgggcata tgatctcgta ttatgcgctg ggggtggtgg
    55741 tcggtgcgcc aatcatcgcg cttttttcca gtcgctactc actcaaacat atcttgttgt
    55801 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    55861 tgctcgccat tggtcggctg gtttccggct tcccgcatgg tgcatttttt ggcgtcggcg
    55921 cgatcgtgtt atcaaaaatt atcaaacccg gcaaagtcac cgccgccgtg gcagggatgg
    55981 tttccgggat gacagtcgcc aatttgctgg gcattccact gggaacgtat ttaagtcagg
    56041 aatttagctg gcgttacacc tttttgttga tcgctatttt taatattgcg gtgatggcat
    56101 cggtctattt ttgggtgccg gatattcgcg acgaggcgaa aggaaaacta cgcgaacaat
    56161 ttcacttttt acgcagccct gccccgtggt taattttcgc tgctacgatg tttggcaacg
    56221 caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    56281 cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg ctggggaata
    56341 tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact
    56401 ttatcattgt actggcactg ctgatgctct ttttcttcgg cggtatgaaa acaacgtcgc
    56461 ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctacaaatat
    56521 tattactgca aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg caaatagcgt
    56581 ttaacctcgg tagcgccgtc ggcgcatatt gcggtggtat gatgctgacg ctggggctgg
    56641 catataatta cgtggcgctg cccgccgccc tgctttcgtt tgctgcgatg tcgtcgttgc
    56701 tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac
    56761 tggggtagat tgcagtcacg gtgcacgttg cttattgccg gatgcggcgt gaacgcctta
    56821 tccggcctac gggactgttc attttgtagg cctgataaga cacgccagtg tcgcatcagg
    56881 catcggagca ccggggggtt gccgccactg tgcgcgttat ttgttgccgg atgcggcgcg
    56941 agcgccttat ccggcctacc ggacggctca ctttgtaggc ctgataagac gcggcaagcg
    57001 tcgcatcagg catgatacgc caattgcctg ggttttttac tcttgtggcc ataaccatgc
    57061 agcgccgcgt acgccgctcg aatcaccatg tttcgccttg tgtaccggcg tttcacattc
    57121 tccgccgaag acaaattgtt taatcaactg cggaaccgtt tgatataaac ggtctacatt
    57181 gctcattccg ccgcccagga caatcacatc cggatcgaga atattcacga catgtgccag
    57241 cgattttgcc agccgcagct cgtagcgacg caatgccagt tccgctaccg gatcgctttc
    57301 ttcaaccagg cggataattt cactgccttt cagcgcatgt ccgctcaaac gacgataatc
    57361 cgtcgcgaat cccgtgcctg aaataaaggt ttcaatacaa ccttgtttac cgcaataaca
    57421 agggacttcc tcgcgataac gcagttcgtc ttcgtccatc cacggtagcg gattgtgtcc
    57481 ccactcgcct gccgtgccat tgccgccgat atgcgcccgc ccattgaatg ccacgcccgc
    57541 gccgcatccc gtgccgataa tcacggcaaa aaccgtctgc gctcccgctg ccgcgccatc
    57601 taccgcttct gaaaccgcca gacagttggc gtcatttgcc agccgcactt cccgctgcaa
    57661 ccgcgcgctt aagtctttat cgaatggctg accgttgagc caggttgaat tggcattctt
    57721 caccacgccg gtgtagggcg aaatcgaacc tggaatgccc atacctaccg ttccgcgctg
    57781 ccctgttgcc tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag tctgccggta
    57841 atcatcacgc ggcgtgggca gacgatggcg gtacaactgc tcccctgcat cgcccaatgc
    57901 aatcacttca gttttggtgc cgcctaaatc gatacctata cgcacggtac tctccttatt
    57961 tttttcaata tcaatagcgt agagacggac aaccggattg gcaatgcaag gccgccgaca
    58021 attcgttatc atgcccgcta aatttaacga caaggccgtg gaaattatca tgctgtggtt
    58081 caaaaattta atggtttacc gtcttagccg cgagatttcg ctgcgtgcag aagagatgga
    58141 aaaacagcta gcctcgatgg catttacccc atgcggcagc caggacatgg cgaagatggg
    58201 ctgggtacct ccgatgggat cgcacagcga tgcgttaacg cacgttgcca atggtcaaat
    58261 tgttatctgt gcgcgcaaag aagaaaaaat cctcccgtcc ccggtgatta aacaggcgct
    58321 ggaagcgaaa atcgccaaac tggaagcgga acaggtgcgt aagctgaaga aaaccgaaaa
    58381 agattcgctg aaagacgaag tgctgcactc tctgctgccg cgtgctttca gccgttttag
    58441 ccagacaatg atgtggatcg atacggttaa cggtttgatt atggtggact gcgccagcgc
    58501 caaaaaagcg gaagatacgc tggcactgct gcgtaaaagc ctggggtcgt taccggttgt
    58561 accattgagc atggaaaacc cgattgaact gacgctgacc gaatgggtac gttccggcag
    58621 cgcggcacag ggcttccagc tgcttgatga agccgagctg aaatcgttgc tggaagatgg
    58681 cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa gagatcacca accacattga
    58741 agccggaaaa gtggtgacta aactggcgct cgactggcag cagcgcattc agtttgtgat
    58801 gtgcgacgat ggttcgctca agcgtctgaa gttctgcgac gagctgcgcg atcaaaacga
    58861 agatatcgac cgtgaagatt tcgcccagcg ctttgatgcc gatttcatcc tgatgactgg
    58921 tgaactggca gcgttaattc aaaacctgat tgaaggatta ggtggcgaag cacaacgtta
    58981 attgctgatt tacctttaat gccggatgcg acgcctgccg cgtcttatcc ggcgtacgaa
    59041 gccacaccag gaatataatc gttagctaca gcgggcaata attcttagcg cagcaatatt
    59101 atgcgtttta tgctgtaact tgctccaggg gcgttgtgtc attgtttttc ctcaagccgg
    59161 tgacgcgctt cactgcgaat ttttcgcgtt gccccggttt tcactaaatc acgtaacagc
    59221 gaaagatcgg gttgttcgac aaataagagt aatgatggat cctcttttaa ccacgccgtt
    59281 ttcacatcgg cagccagctg tgaattatta atagccagcg accgctcctg aggctcagtc
    59341 aatgtcgtta acaacaacac cggggtatgt gcgttgtgca atgccgtttg gcgcaaatga
    59401 tcattgcgac tttgcagcaa tgcacgccac cattcatcat ctgatttatc ttttatcgtc
    59461 gccagttccg caacaatatc tggagatctt cgctgatatt gatatcaacc ttgatgaatt
    59521 atgaatatat cggaaatcga aaaaatccca gcaaaggaaa tattattatg ggttaaagag
    59581 aggacatttc agaaaaatgc cctcccgttc aaggggaagg cgaggaatta cagatagcgg
    59641 cacagataag aggtgggttc ggcaacttgc agatgaaact cgctgtgacc gggaacatta
    59701 aacaccgaac cggcttcata cacttgccag tcggtcgcgt caggcagtaa cacattcagc
    59761 gcgccactga ttaccgtcat ctcttcaggc tcagcggtgc tgaaggtgta ttcaccttca
    59821 accataacac ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat cgatttcact
    59881 ttgccggaaa agtactcatt actttgaagc ataaactggc cctgtctggt agttgaacat
    59941 ctacttcact ataggggcca gaggcgctgg ctgtcacgca aaattacatc agtaattccg
    60001 cagccagccg tgcaatcaat acgttagaga gcaagacagg aacatcgagc tgtttttgca
    60061 gtaaatcgcg atgacgctgg ttaaatccca aacaatccag catgatgaca tctgcacctt
    60121 ttgccagtaa ttctttcccg gcatcaatga ttttttgttc cgaatcatga atggggttac
    60181 ccaatgaaaa tactggcggt ttctgcaaaa tttgccattt ttgcgcctga acggtcagca
    60241 actcctcaac cggtacgata acccccacct gatgatcttc aacaatagag gaaaccagcg
    60301 gtggcaatat ccgcgacggc tcaagaaaga tcgtattacg cgcggtcata ctactaatgt
    60361 ttgctgtact cattaatata atgacgtcgt aaccctgatt atcgagcact tcaaccacac
    60421 cttgcaggtc tcgctcaact ttgcgacgcg aaacatgggc cagatggttg tcatttaata
    60481 atgtgagaat tgtgtcttcg cctgcttctg gcgcgtactc cgccatcact tcttcgcggc
    60541 ttaacttccc cagcaggcta tgatgggaaa tattatcttc gtcaatgtat tctgtcagga
    60601 gcggcaaaac ttcctgcata ggtacaatgc cgatagtcag gatcgccaac gacgcactca
    60661 ttgttcttcc accttacttt tatttcgtcg ttaattaccg ggcaagtgtt aagcaccact
    60721 gctgacactt acttgtgcta caaatgacaa aaagcgtagc agcagacctg cactgcataa
    60781 tgtaaagatt ccaaatgatt ccagtaatgg attgttattg tttaatattc taattatttg
    60841 aaaaacatga attgtgaaaa atgtgacgca gatcccttgt ggcggcactc agaatccctt
    60901 ccgagcggtc tgatgcggtc actctttgtc agggtcctca tagcgttttt tcgcatccat
    60961 cgcttcctga gcggtcggat gttcactgat caacgagtct ggtttaggat gatcggctct
    61021 gagttggtac caggttaccg tctgtcctgg ctttcctttt tcaatcgtca ctatatatgc
    61081 ttcacgagga taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt
    61141 aagtataggc gctcgaaaat caacaattga tcgtctgtgc cagagcgctg cgaatttcag
    61201 aaatcacctg gctgggttcg tttgttgcgt cgatgataat atgcgcaact tcgcgatata
    61261 gcgcatcgcg ttcttccagc acttcctgaa cttcttcgct cagtggtttt cccgttaagg
    61321 ttggccgtaa atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg
    61381 cacacaaata aaccacgatc ccgttatttt gcatgaagtg acgattaaat tccgtcagaa
    61441 taatgccgcc gcccgtggcg ataacggtgg atggcgcagt taccgcttcc agtgccgccg
    61501 tttctctggc gcggaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat
    61561 tgagctgtga ttgcaaccac tggtcggtat cgacaaaccg acggttaagc gaatcggcaa
    61621 gggccattcc gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg
    61681 tcatcgtggg ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatggc
    61741 gatcatacca tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtga
//
LOCUS       DAPQZD010000007        61429 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPQZD010000007 DAPQZD010000000
VERSION     DAPQZD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.007"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(135..416)
                     /locus_tag="RO454_000477"
     CDS             complement(135..416)
                     /locus_tag="RO454_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDY0932360.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(679..1788)
                     /gene="apbC"
                     /locus_tag="RO454_000478"
     CDS             complement(679..1788)
                     /gene="apbC"
                     /locus_tag="RO454_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDY0932361.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1920..3953
                     /gene="metG"
                     /locus_tag="RO454_000479"
     CDS             1920..3953
                     /gene="metG"
                     /locus_tag="RO454_000479"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDY0932362.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4094..7894
                     /locus_tag="RO454_000480"
     CDS             4094..7894
                     /locus_tag="RO454_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDY0932363.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPLMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            7907..11668
                     /locus_tag="RO454_000481"
     CDS             7907..11668
                     /locus_tag="RO454_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDY0932364.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            11678..15310
                     /locus_tag="RO454_000482"
     CDS             11678..15310
                     /locus_tag="RO454_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDY0932365.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15371..15687
                     /locus_tag="RO454_000483"
                     /pseudo
     CDS             15371..15687
                     /locus_tag="RO454_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16389..17477
                     /locus_tag="RO454_000484"
     CDS             16389..17477
                     /locus_tag="RO454_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDY0932366.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17488..19767
                     /locus_tag="RO454_000485"
     CDS             17488..19767
                     /locus_tag="RO454_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932367.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            19760..20896
                     /locus_tag="RO454_000486"
     CDS             19760..20896
                     /locus_tag="RO454_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDY0932368.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRYSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20893..22896
                     /locus_tag="RO454_000487"
     CDS             20893..22896
                     /locus_tag="RO454_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDY0932369.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            23021..23482
                     /locus_tag="RO454_000488"
     CDS             23021..23482
                     /locus_tag="RO454_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDY0932370.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23523..23993)
                     /locus_tag="RO454_000489"
     CDS             complement(23523..23993)
                     /locus_tag="RO454_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDY0932371.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(24040..24759)
                     /gene="yehT"
                     /locus_tag="RO454_000490"
     CDS             complement(24040..24759)
                     /gene="yehT"
                     /locus_tag="RO454_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDY0932372.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24756..26441)
                     /gene="btsS"
                     /locus_tag="RO454_000491"
     CDS             complement(24756..26441)
                     /gene="btsS"
                     /locus_tag="RO454_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDY0932373.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26663..27394
                     /gene="mlrA"
                     /locus_tag="RO454_000492"
     CDS             26663..27394
                     /gene="mlrA"
                     /locus_tag="RO454_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDY0932374.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27454..27561
                     /locus_tag="RO454_000493"
     CDS             27454..27561
                     /locus_tag="RO454_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDY0932375.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27542..28273)
                     /gene="yehW"
                     /locus_tag="RO454_000494"
     CDS             complement(27542..28273)
                     /gene="yehW"
                     /locus_tag="RO454_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDY0932376.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28278..29204)
                     /gene="yehX"
                     /locus_tag="RO454_000495"
     CDS             complement(28278..29204)
                     /gene="yehX"
                     /locus_tag="RO454_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDY0932377.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(29197..30354)
                     /gene="yehY"
                     /locus_tag="RO454_000496"
     CDS             complement(29197..30354)
                     /gene="yehY"
                     /locus_tag="RO454_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDY0932378.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30361..31278)
                     /gene="osmF"
                     /locus_tag="RO454_000497"
     CDS             complement(30361..31278)
                     /gene="osmF"
                     /locus_tag="RO454_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDY0932379.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31468..33765)
                     /gene="bglX"
                     /locus_tag="RO454_000498"
     CDS             complement(31468..33765)
                     /gene="bglX"
                     /locus_tag="RO454_000498"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDY0932380.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            33961..35676
                     /gene="dld"
                     /locus_tag="RO454_000499"
     CDS             33961..35676
                     /gene="dld"
                     /locus_tag="RO454_000499"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDY0932381.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35715..36647)
                     /gene="pbpG"
                     /locus_tag="RO454_000500"
     CDS             complement(35715..36647)
                     /gene="pbpG"
                     /locus_tag="RO454_000500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDY0932382.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36821..37408)
                     /locus_tag="RO454_000501"
     CDS             complement(36821..37408)
                     /locus_tag="RO454_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDY0932383.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37578..38156
                     /locus_tag="RO454_000502"
     CDS             37578..38156
                     /locus_tag="RO454_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0932384.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38286..39047)
                     /locus_tag="RO454_000503"
     CDS             complement(38286..39047)
                     /locus_tag="RO454_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0932385.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(39100..40536)
                     /gene="mdtQ"
                     /locus_tag="RO454_000504"
     CDS             complement(39100..40536)
                     /gene="mdtQ"
                     /locus_tag="RO454_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDY0932386.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40760..40843
                     /gene="yohP"
                     /locus_tag="RO454_000505"
     CDS             40760..40843
                     /gene="yohP"
                     /locus_tag="RO454_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDY0932387.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41231..42181)
                     /gene="dusC"
                     /locus_tag="RO454_000506"
     CDS             complement(41231..42181)
                     /gene="dusC"
                     /locus_tag="RO454_000506"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDY0932388.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            42420..42818
                     /locus_tag="RO454_000507"
     CDS             42420..42818
                     /locus_tag="RO454_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDY0932389.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42815..43510
                     /locus_tag="RO454_000508"
     CDS             42815..43510
                     /locus_tag="RO454_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDY0932390.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43640..44524
                     /gene="cdd"
                     /locus_tag="RO454_000509"
     CDS             43640..44524
                     /gene="cdd"
                     /locus_tag="RO454_000509"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDY0932391.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44674..45393
                     /gene="sanA"
                     /locus_tag="RO454_000510"
     CDS             44674..45393
                     /gene="sanA"
                     /locus_tag="RO454_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDY0932392.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45396..45635
                     /locus_tag="RO454_000511"
     CDS             45396..45635
                     /locus_tag="RO454_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDY0932393.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            45829..47067
                     /gene="preT"
                     /locus_tag="RO454_000512"
     CDS             45829..47067
                     /gene="preT"
                     /locus_tag="RO454_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDY0932394.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            47061..48296
                     /gene="preA"
                     /locus_tag="RO454_000513"
     CDS             47061..48296
                     /gene="preA"
                     /locus_tag="RO454_000513"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDY0932395.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(48539..49549)
                     /gene="mglC"
                     /locus_tag="RO454_000514"
     CDS             complement(48539..49549)
                     /gene="mglC"
                     /locus_tag="RO454_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDY0932396.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(49565..51085)
                     /gene="mglA"
                     /locus_tag="RO454_000515"
     CDS             complement(49565..51085)
                     /gene="mglA"
                     /locus_tag="RO454_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDY0932397.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(51146..52144)
                     /gene="mglB"
                     /locus_tag="RO454_000516"
     CDS             complement(51146..52144)
                     /gene="mglB"
                     /locus_tag="RO454_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDY0932398.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKTYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(52424..53464)
                     /gene="galS"
                     /locus_tag="RO454_000517"
     CDS             complement(52424..53464)
                     /gene="galS"
                     /locus_tag="RO454_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDY0932399.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(53606..54763)
                     /locus_tag="RO454_000518"
     CDS             complement(53606..54763)
                     /locus_tag="RO454_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDY0932400.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(54780..55448)
                     /gene="folE"
                     /locus_tag="RO454_000519"
     CDS             complement(54780..55448)
                     /gene="folE"
                     /locus_tag="RO454_000519"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDY0932401.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            55706..56542
                     /gene="yeiG"
                     /locus_tag="RO454_000520"
     CDS             55706..56542
                     /gene="yeiG"
                     /locus_tag="RO454_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDY0932402.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(56574..58565)
                     /gene="cirA"
                     /locus_tag="RO454_000521"
     CDS             complement(56574..58565)
                     /gene="cirA"
                     /locus_tag="RO454_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDY0932403.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(58857..60326)
                     /gene="lysP"
                     /locus_tag="RO454_000522"
     CDS             complement(58857..60326)
                     /gene="lysP"
                     /locus_tag="RO454_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDY0932404.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(60531..61412)
                     /locus_tag="RO454_000523"
     CDS             complement(60531..61412)
                     /locus_tag="RO454_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0932405.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
BASE COUNT        15262 a        16178 c        15344 g        14645 t
ORIGIN      
        1 acattaaata tttatcaaat ctattaatta atgtggattt ctataaattg tattattaat
       61 atcaataaaa aagcttaaac cattcatgta aatgatttaa gctttctaaa tccgattaaa
      121 accagggtgg caaattaatc gtagcgattt aacacgattc gacgtttgct atcgctatct
      181 gcagcaatca cttccgtcgt tacaccttta actggcgaag aaacctgatg acgggtatca
      241 acattatacg ttaactgacc acaatgctgt tgaggttgac cattaaccat agccatactg
      301 catgtcgggg gactgatcct accattaatc gttaatgtgg cagtttctga agagaacgcg
      361 ggagaagcca atccataggc aagaaaaata attcctgaca gccagtactt attcattgaa
      421 cgttatccct gtagtaaagg ttatgcctgg aaagaatgag tgcataacaa actatagctg
      481 tacatccact acacagccac gaaggatgat aatgaagcat cgccggtacg atcaatcgac
      541 tttgtagagt ttcggacgaa ggtccgcaga atattcgcaa cattaaataa gtgttcatta
      601 atgttgcaaa aacaaataca ccgcatcaat gtaacatctc tgcggtgtat gaagaataac
      661 cagaatggtt aatacgcgtt agaccgcgcg gaaggaaatc tcgccaggaa tgacttcacc
      721 ctgccagtag agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc
      781 gctctcagga cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat
      841 gtgtaacggc atctgcccca gcagctgcgt gtggtatttc tcggccagtt tctctgcacc
      901 accggtaccg aaaatcggct catggtgacc acagttactg caaatatgca cactcatgtt
      961 ctcgacaata ccgagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc
     1021 atcgatcaac gcgatgtcct gcggggtggt gaccactacc gcgccagtta ctggaatgtt
     1081 ttgtgccagt gtcaattgaa tgtcaccggt acctggcggc atatcaagca ccagatagtc
     1141 gagatccggc cacaaggttt cctgcaacat ttgcattaac gccttgctcg ccattgggcc
     1201 acgccacacc atcgcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc
     1261 atgagacata atcggtgcca tatgggtacc gtccggcgaa gttggacgct gattttctgc
     1321 gcccagcatg gtagggattg atgggccgta gatatcggcg tccagaatgc cgactttcgc
     1381 cccttctgcc gccagtgcca acgccaggtt taccgccgtg gaggatttac ccacgccacc
     1441 tttaccggaa ctgaccgcga taatgttctt cacgccgtta attcccggct gattcttcac
     1501 gcgtttcaga gtggctatgt tatgcgacag tttccagtcg atagctttcg ctccggtgat
     1561 gcgcagcagt tcggcgctac attgctcttt taactcttcg aacgcgctat gccagacgaa
     1621 tggcatgacc agttccacat gcagcgtgtc gtccatccag gcgacatggt gcaacgcttt
     1681 cagcgttgtg aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac
     1741 cattgcgcgc aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt
     1801 tttgtataga taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt
     1861 aacattttcc catttggtac tatctaagcc cttttcacta ttaagaagta atgcctacta
     1921 tgactcaagt cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa
     1981 tccacctcgg ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa
     2041 tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc ccacggcacg ccgatcatgc
     2101 ttaaagctca gcagcttggt atcaccccgg agcagatgat tggcgaaatg agtcaggaac
     2161 atcagactga tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg
     2221 aagagaatcg ccagttgtca gaacttatct actctcgtct gaaagaaaac ggttttatta
     2281 aaaaccgcac catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt
     2341 ttgtaaaagg cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag
     2401 tctgcggcgc gacctacagc ccgactgaac tgatcgagcc gaaatcggtg gtttctggtg
     2461 ctaccccggt aatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa
     2521 tgctgcaggc atggacccgc agcggtgcgt tgcaggagca ggtggcaaat aaaatgcagg
     2581 agtggtttga atctggcctg caacagtggg atatctcccg cgacgcgcct tatttcggtt
     2641 ttgaaattcc gaacgcgccg ggcaaatatt tctatgtctg gctggacgca ccgattggct
     2701 atatgggctc tttcaagaat ctgtgtgaca agcgcggcga cagcgtaagc ttcgatgaat
     2761 actggaagaa agattccact gccgagctgt atcacttcat cggtaaagat atcgtttact
     2821 tccacagcct gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc
     2881 tgtttgttca cggctatgtg acggtgaacg gcgcgaaaat gtccaagtct cgcggcacct
     2941 ttattaaagc cagcacctgg ctgaatcatt ttgacgccga cagcctgcgc tactactaca
     3001 ccgccaaact gtcatcgcgc attgatgata tcgatctcaa cctggaagat ttcgttcagc
     3061 gcgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc gtcccgtaac gctggcttta
     3121 tcaataagcg ttttgacggt gtgctggcaa gcgagctggc tgacccgcag ttgtacaaaa
     3181 ccttcactga tgccgctgaa gtgattggcg aagcgtggga aagccgtgaa tttggtaaag
     3241 ctatccgtga aatcatggcg ctggctgacc tggctaaccg ctatgtcgat gaacaggctc
     3301 cgtgggtggt ggcgaagcag gaaggtcgcg atgccgatct gcaggcgatt tgctcaatgg
     3361 gcatcaacct gttccgcgtg ctgatgactt acctgaaacc ggtactgccg aaactgaccg
     3421 agcgcgcaga agcattcctc aatacagaac tgacctggga tggtatccag caaccgctgt
     3481 tgggccataa agtgaatcca ttcaaggcgc tgtataaccg catcgatatg aaacaggttg
     3541 aagcactggt ggaagcatcc aaagaggaag taaaagccgc tgccgcgcca gtaactggcc
     3601 cactggcaga tgatccgatt caggaaacca tcacctttga cgactttgct aaagttgacc
     3661 tgcgcgtggc gctgattgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc
     3721 tgacgctgga tctcggcggt gaaaaacgca atgtcttctc cggcattcgt tctgcttacc
     3781 cggatccgca agcactaatt ggtcgtcaca ccattatggt ggctaacctg gcaccacgta
     3841 aaatgcgctt cggtatctcc gaaggcatgg taatggctgc tggtcctggt gggaaagata
     3901 tcttcctgct aagcccggat gccggtgcta aaccgggtca tcaggtgaaa taatccccct
     3961 tcaaggcgct gcatcgacag cgccttttct ttataaattc ctaaagttgt tctcttgcga
     4021 ttttgtctct ctctaacccg cataaatact ggtagcatct gcattcaact ggataaaatt
     4081 acagggatgc agaatgagac actttatcta tcaggacgaa aaatcacata aattctgggc
     4141 ggtggagcag caaggcaacg agttacatgt cagttggggc aaagtcggca ctaacgggca
     4201 aaaccaggta aaaagctttg ctgatgctgc ggcggcggaa aaagcaaagc ttaagctgat
     4261 cgcagagaaa acgaaaaaag gctatgtgga aaatgcttct gcaaacgtgc atatcacccc
     4321 catcaccaaa gccactcctg aagttgaaac ttctcctgag agtaaaaacc aacgcccctg
     4381 gctggctgat gatgcggtca ttcggctaac tgacgatatt aatcgatttg cttttcctca
     4441 tcgctctcgc cccagagaga tcaattatct gcacaaagat agtcaaatat ggcagcgcat
     4501 cgcgaataac accagggcat atgatcccgc caacaattac cgttcttatc cagaaaactg
     4561 gcagcaggct tttgctgagt tacaaaggcg aattctggat aatcaacaga cagggagtgc
     4621 gcaatctgat gcggcattgc tctggagttt ctggaattct tactctgcag atgaactagt
     4681 cgatgactta atcattcgct gtggtatgga aagtgccgtt gaaattgccc ttctcgctct
     4741 tcaactgaga tataaaccag taaaaggcgc agtaaccacc atcattccac ctaatcacga
     4801 agcagaatcg ctaccgagtt ggcatcaacg cctatgtcat catctttcac tcgcttcaga
     4861 agatgagtgg caacgctgtg tagataaagt actcgcggct attacgacgc tatccccagc
     4921 acggcaacct tttgcggcgt tactgctccc tgaacgcccg gatattgcca atgctatggc
     4981 tctacgctat gcagatcaaa acgttccggc gatgacctgg ttaagactgg tagtaaccga
     5041 tgaagccgct ctgactgcag ccgaaaaata cgactttcca ccgttatatc gtgattttcg
     5101 cgcttatctg gcaatgttgc tggcaaataa tggggtgagc ggtgtaagcc ggatacttac
     5161 tgagtttgtg gaagagcatt ctgacaatcc cacttatctc ttcgagcgca tcagcgagac
     5221 tgaagatctt gtcaaatggt tatggaaaac gaatcacccg gatgcgattc aaattctgat
     5281 cctcggtgta attggcaaga aaaagcacct ggaatactta agcaaagcat gtcaaaaaca
     5341 tcccgctgcg gctattgccg cttatgcaac tttgctggca atacatgaag ataaagagtg
     5401 gcataaagcg cttgtcaaat tgattaccat cacgccagag ttagtatgcg acgtcattcc
     5461 ctgggttagt gctaaagcgg caggcatctt atctgaatgt cgtcctcagt cagtagctga
     5521 agaatgtgag tatgccactg cggatatgtt gccagagctg tttgtttcgc cgccctggat
     5581 gactaaagag aaaaagaaaa attcgccggt gtttgatctt cctgtactgc cagttccttc
     5641 tgtttctgac gtcactccag agattgccaa aaaactaacc cgtacatacc ttgtcacccg
     5701 cttccagcaa atagcccaac agcaggcaac aaaacaaata ttgtttaccg acctgccgct
     5761 gatggaaaaa gcaagctggg agagacactt agtccccctc acaccagaac agcaaattct
     5821 gtggtgtctg ggttttgaca aatggcggga gagcggagaa aaaatatacg aaaaaattcc
     5881 ggcgccacaa agtgccgttg atgccctgct acgcttcgat tttccagcac tcaatgctga
     5941 atttgcgcaa taccataaca atgcttacaa aagctggaat ctcattgccc tctgttatct
     6001 acctggtcaa caggccattt cttttcttaa ccagatagtc aaagaagata actactctgg
     6061 tgagggatat attctggcca tttttggtag cgccgccatt cctgcattta tgacctgcct
     6121 gcaacgtgag ccacaacggt tgtggttttt caccttcttc cttggtgtca atgaactggc
     6181 attgccaatg gcgcaacgac ttcagaaaaa aatgtcgtat gaagatgcac gaaactggct
     6241 aacggattac ccacgccatg ccgccgccgg gctacttcct gtggcactgg gcaaacaagg
     6301 aaaagatcgt gattgtgccc gccaggcgct ccgtctgctg gtgaatttaa accagcgaga
     6361 aacgattgag gaaatcgccc aaggatacaa ccaacctgat gttttagctg cgctggcaac
     6421 attatttgat agcgagccgc tggaagaata tcccgctaaa atcgccccac tgcccggctt
     6481 ttatcagttc accttatggc gcagaccacg gcttaaaagt aacaaccagc ctctgccaga
     6541 tgacgctatg cgccacctcg gcataatgct gagctttcca cgcgacatta ccccctacgc
     6601 agggcttgcg atcatcaaag aaaccttcac ccgcgaatca ctggctgaat ttggctggga
     6661 tctgtatacc gcctggacag aagctggcgc acccgcaaaa gaaaactggg catttacctc
     6721 gctggggatt ttaggcaatg acgacaccgc gcgtaaatta accccgctca tccgcacctg
     6781 gccgggtgaa tcccagcata aacgcgcggt ttacgggctg gatgtgttag ccagtatcgg
     6841 tagcgacatt gcgctaatgt tgcttaatgg cattgcgaaa aagattaaat tcgttgcatt
     6901 acaggaacat gcctgcgaca aaatcaacat ggtcgctgaa aatcgcgggc tgactatggc
     6961 tgaactggaa gaccgcctgg caccggattt aggacttgat agcagtggct cgttgacact
     7021 ggatttcggc ccacgtcaat ttaccgttgg ttttgacgaa actttaaaac ccgtggttcg
     7081 cgacgcaaac ggcaaagttc tgaaagattt acccaaacca aatcagagcg atgataaaac
     7141 gctggcaact gacgcggtta atctcttcaa acagttgaaa aaagatgtac gcgccatagc
     7201 cagccagcag attgatcgtc tggagcaggc tatgtgccag cgcagacgct ggacggcaga
     7261 gcagttccgc ctgttcctgg tggagcatcc gctggtacgt cacttaaccc gacggttgtt
     7321 atggggggta tatactgagg aaaacacgct gattgcctgc ttccgggtcg cagaagacag
     7381 cacttacagc gatgcgcagg atgagctatt tacgttgcca gcaggaaaca tcggtattcc
     7441 gcatgtgctg gaaatatctc ctgaatccgc cgctgcattt gggcaaattt atactgatta
     7501 cgaacagctt cctccgttcc gccaactcga tcgtggttat taccaccttg ctgataatga
     7561 acgtgattcc catgaattga tacgctggca aggacgtctt tgccaggccg ggcgcattgt
     7621 cgggctggaa cgccgaggct ggcaacgtct ggaagaaagc ggcagcgttt acgcgatgcg
     7681 caaatctact ccttatggcg atctcgaact tgaaacagaa cctttttcat taatttatgg
     7741 tgaaacgggg tatggcgacc agctaccggt tgaaagcgta aaaatcacct cgcctgataa
     7801 tcgctacggt aaacaatcct cactcacttt ttccgcgctc gacgacatca ccgccagcga
     7861 actcattaac gacattgaat cactgtttga ttaaaaggat ttagcaatga aaacctatat
     7921 ttatcaggac gaaaaatcac ataaattctg ggcagtagag cagcaggaaa atgaactgca
     7981 tatcagttgg ggcaaagtcg gcactaacgg gcaaagccag ataaaaagct ttgctgatgc
     8041 ggcggcggcg gaaaaagcgg agctgaagtt gattgcggag aaaacgaaaa agggctacgc
     8101 agaagaagta tccgtgacga caccgacatc tgcccccgtc caggttatcg catgctctga
     8161 aaaagcccct ttgccgcaag acaaaccacc atttgctgaa gaccaccttc cctggctggc
     8221 agacgacgcc caaatcattt taccgacaga ggtcgcacca accacactaa gtcatcgacg
     8281 ctggcctggc gattctgtgc cacaggagaa tgaattaact ctgctacgct ccgtcgccgc
     8341 gcatactcac cggcgtttta aaaaggtgat caccttcgat tacagcacct gttcactgga
     8401 ctggcagcag gcgattacac aagcagtggg ccttatcgac tcaccgatta gcaccgcact
     8461 accaccgatg gttctggcgg tactggttgc gctggaacaa ggcttcaatc gcaacgatca
     8521 cgaagagtta atggatcaaa ttgtccagga gggtggtctg gaatatgcca ctgaggttgt
     8581 cattgcattg caagccatcc gctttgactg gaattatgat gcccacctca ttacgtttac
     8641 cccggataac aagcagcctg gctatctgtc acgctttgcc tcggttgaaa tgcgtctgcg
     8701 taaacatctc tcactagcaa atgacgatgt atggcagcgc tgcgccgaca aactgattgc
     8761 agctctcgga aacatacccg cctggcatca gccgctggtt gcgttattgt tgccggaaaa
     8821 acaagatgtg agccatgaga ttgccagacg tctttgcgga caaaaagggt tatatgcgct
     8881 ggaatggcta aaactaacgg ccgccgacga gcaggtattg gcagaactgg ggaaatacta
     8941 tccaggacag ccagggcagg tatttgatga ttattacggc ggtaaaatct ggtgcgcaac
     9001 gatactcaag gagcaaggtg tcggtgcgct ggcccgtttt gctccttacg ctgctggtga
     9061 cacatgtggt gaagtgttga tgcatatcaa ccacccgcag gcacttacac tacttatcca
     9121 tgcaagcgag cagggaaagc gttgccacga tcgcatgaca aagactttcg ttcgctttcc
     9181 acatgcagcg ctggcggcat tagcagaact tctggcacaa aaagatcaaa agcgttggcg
     9241 catgatgcta atgacaatgc ttatctcaca accaacatta gcagagcgag taatcccctg
     9301 gctctcaaca cccgcagttg ccgtactgaa atcatgccag caacaactga cacagccctc
     9361 aaaccatgcc agcgctgata tgctgcctgc cgtattagtc tcaccgccct ggctttcgaa
     9421 aaagaaaaaa tctgtaatgc cggtattgga tttaacgcca ttaccgctgg agtcatgctg
     9481 cacattgacc gagacggcag aaaaagagat tcatgcaagg catcgctggc acgcacatca
     9541 aatagacatc ggccaaaaag aggatattca gaactacctg acacgtctgg gtttcaatcg
     9601 ctggaataat ggccagtata tgaaagcgtc tgatgctgtt gtagaactct ggcagagggg
     9661 agattattct gcgctaatca gcgaatttaa aaccttctgg cactcctatc aacgggaatg
     9721 gcagctttac atgttagccg ccctgcctat cgaaaaaaca gcgcaggcgt ggaatgtcct
     9781 cagtaaagag ccacacgtcg gtgtcgagtt tgtcatgacg cacctgcaac tcgctgggtt
     9841 acagggtttt attcactcct tttcacgcta tccacaagaa gctctgcctg tagcacaata
     9901 ttttgcggct atcgaattag cgccactgat cgcccgcgcc ttcaacaaac tcaaaacatt
     9961 acgacaagac gcccgtatct ggctgctgaa atacccggaa cacgccatta ccggcctgct
    10021 gcctgcagcg ctcggcaaaa ccggtgaagc acaggacaac gcccgtgctg ccttgcgtat
    10081 gcttaccgaa aacggtcatc agccattgct gcaagaaatc gcccggcgtt ataaccagcc
    10141 ggaagtaacc gatgcggtga acgctctact tgcgctcgat cccttagata atcacccgac
    10201 aaaaatcccc actcttccga ccttttatca gccatcgctc tggacgcgcc cgttattaaa
    10261 agcaaacgcc caatcactgc ctgatagcgc actcctccac ctcggtgaaa tgctccgctt
    10321 ccctcaggaa gaggcactgt atccaggatt attgcaggtg aaagatgcct gtaccaccga
    10381 ctcactggct gaatttgcct gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc
    10441 gaaagagagt tgggcgttca ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa
    10501 actgacgcca ttaatccgtg cctggcctgg cgaatcccag cataaacgtg ccaccgttgg
    10561 gttggatatc ctcgccgcta tcggtagtga tatcgccctg atgcagctta acggcatcgc
    10621 ccagaaactg aaattcaaag cattacagga gcgggcaaaa gaaaaaatag ccaacattgc
    10681 cgagagccgc gaactcacgg tggcggagct tgaagatcgg ttagcaccgg atctcggtct
    10741 ggatgataac ggttcgttgc tgctggattt cggcccacgc cagttcaccg tcagctttga
    10801 tgaaacctta aaaccgtttg tgcgtgacgc ttccggcagt cgtctgaaag acctgcccaa
    10861 accaaacaaa agcgatgatg aatcacaggc gaacaatgct gttaaccgct acaaattgct
    10921 gaaaaaagat gcgcgtaccg tcgccgccca gcaggtagca aggctggaat ccgccatgtg
    10981 cctgcgtcgc cgctggtcac cggaaaactt tcagctcttc ctggttgagc atccgctggt
    11041 ccgccactta acccgccgtc tgatttgggg cgtttatagc gccgaaaacc agctactggc
    11101 ttgctttcgc gtagcggaag ataacagcta cagcaccgct gacgatgatc ttttcaccct
    11161 gccggaaggc gatatctcta ttggtattcc tcacgttctg gaaatatcac cgacggatgc
    11221 tgccgccttc gggcagcttt ttgccgacta cgaactgtta ccaccgttcc gccagcttga
    11281 tcgtaacagt tacgcgctga cagaagccga gcgcaatgcc agcgaactga cacgctgggc
    11341 aggcagaaaa tgcccgagtg gtcgggtaat ggggctggca aataaaggct ggatgcgtgg
    11401 tgaaccgcag gacggtggct ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt
    11461 aatcatggaa atcgatgaag gttttgcggt cggcatgtcg ccagccgaac tcagtgccga
    11521 gcaactctta agcaagctgt ggctatggga aggcaaagcg gaaagctatg gctgggggag
    11581 taattcaaca caggaagcgc agttctccgt actcgatgcc atcaccgcca gcgagctaat
    11641 taacgatatt gaagccctgt ttgaataagg aaagagcatg gacaaggaat taccgtggct
    11701 ggcggataac gcccaactgg aactgaaata taaaaaaggc aaaacaccac tcagccatcg
    11761 caattggcca ggcgaaccgg tacctgttat cactgaaaac atcattcaga cactgggtga
    11821 tgaattgcta caaaaagcag agaagaaaaa aaacattgtc tggcgttatg aaaacttttc
    11881 actggagtgg cagtccgcca tcacgcaggc catcaacttg atcggcgaac acaaaccctc
    11941 aatcacggcc cggacaatgg cggcgctagc ctgtatcgcg caaaatgata gccaacagtt
    12001 gctcgacgaa atcgtccaac aagaggggct ggaatatgca actgaggtgg tgattgcacg
    12061 ccagtttatt gtacggtgtt atgagagtga tcctctggta gtaacattgc aatatcagaa
    12121 cgaggactat ggctatggtt atcgctcaga aacctataac gaattcgatc tccgattgcg
    12181 taagcatctc tctctggcag aggaaagctg ctggcagcgt tgcgccgaca aactcattgc
    12241 cgcactgcca ggaataacca aagttcgccg cccttttatt gcgctgaacc tcccggaaaa
    12301 acccgaaatc gccaacgagc tggtaagcct tgaatgttcg caaactcatt ttcgctcaaa
    12361 agagtggtta aaagttgttg ctgatgaccc taaggcggtg aaagaactcg cacgctactg
    12421 gagccaggat atatttagcg atcgagaagc cagctacatg tcgcatgaaa accacttcgg
    12481 ctacgcggcc tgcgctgccc ttttgcgcga acaaggactg gcagccgttc cgcgcctcgc
    12541 gatgtatgca cataaagaag attgcggcag tctgctggta caaattaacc atccgcaagt
    12601 catccgcacc ttgctactgg tggctgataa aaacaaaccc agcctgcaac gtgtggctaa
    12661 atacagtaaa aacttccccc atgcgacgct cgccgcactg gcagagctgc tggcgttaaa
    12721 agccccccct gcgcgccctg gttatcccat catcgaagac aaaaagctgc ctgcacagca
    12781 aaaagcacgt gatgaatact ggcatacgct gttacaaacg ctgatggcat cgcagccaca
    12841 actggcagaa gaggtgatgc cgtgcttatg tacacaagcc caggcagtgg tgaatggtta
    12901 tttatcggca tcgccaaaac ttgctttcga gagtactcat agcaacgacc tgccagaaat
    12961 acttgtttct ccaccgtggc gaggtaagaa aaaaacggca ttgctgcgcc ttgatttagt
    13021 gcctcttgaa ttagcgccca aagcccgctg gcaaccaggc gaacgagaga gacttgccgc
    13081 cacagagtct gcccgttatt tcagcacggg atcttttacg gaacgcatgg aacgaaaaag
    13141 tggtcgagtt gtactacagg agctaggttt tggggatgat gtatggctgt tccggaatta
    13201 tatactgccc ggcaaactgg atgcggcgcg caaatcatta gtgggtcagt ggcattactc
    13261 cccgcgcaga gtagaggaaa tcaacaatgg ctggcactcc acggaagcaa aatcagccga
    13321 acaggcgctt cgttccggcg acgttgaagc gttaattaac acctgggaaa atgatagcta
    13381 ctcgcattat cgtcaggaaa agagtgtctg gaacctgtat ttattggcgc agttgccacg
    13441 tgagatggct ttgactttct ggctgcgtat caatgagaaa aagcatctat tcgcgggtga
    13501 ggactatttt ctcagtatcc tcgggttgga tacattacct ggcctgttat tggctttttc
    13561 acatcatcca aaagaaacat ttccgttaat tttaaatttc ggcgcaacag aactggccct
    13621 gcccgttgcc cgcgtctggc gacgtttcgc ggcgcagcgt gatctggctc gccagtggat
    13681 tttacaatgg ccggaacata cggctactgc acttatccct cttgtcttta caaaacccag
    13741 cgataatagc gaagctgcat tacttgccct gcgtttactg tatgaacaag ggcatggcga
    13801 attactgcaa accgtggcaa accgctggca gcgtacagat gtatggcctg ccctggagca
    13861 attacttaaa ctgggtccaa tagaaattta cccggcacgt attccaaaag ctcctgattt
    13921 ctggcatccg ggaatgtggt ccaggccgcg ccttatcact aataatcagc ctgttaccga
    13981 tgacgctctg gaaattatcg gcgaaatgct gcgctttacc cagggcggac gtttttatgg
    14041 cggactggaa cagctgaaaa cgttttgcca gccgcaaacg ctggcagctt ttgcctggga
    14101 tcttttcacc gcgtggcaac aagctggtgc ccccgcaaaa gacaactggg catttctggc
    14161 gttaagtctc tttggtgacg aaaccacggc acgagatctg acgacacaga tcctcgcctg
    14221 gccacaagaa ggcaaatctg cccgtgctgt cagtggcctg aacatcctta ccctgatgaa
    14281 taatgatatg gcgctgatac agctgcacca tatatcccaa cgggcgaaat cccgctcctt
    14341 acgtgataac gcggcggaat ttcttcaggt ggtcgcagaa aatcgcgggc taagccagga
    14401 agagttagcg gacagattag tcccaacatt gggccttgat gatccgcagg cgttgagttt
    14461 tgattttggt ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc cggttatctt
    14521 tgatcagcaa aacgttcgcc agaaaagtgt tccccggttg cgcgccgatg acgatcaact
    14581 gaaagcgccc gaggcactgg cccgactaaa agggctaaaa aaagatgcca ctcaggtgag
    14641 caaaaacctg ctcccacgtc ttgaagcagc cctacgtatc acccgccgct ggtcgctggc
    14701 agattttcat tctctgtttg ttaatcatcc ctttacccgc ctggttaccc agcgattaat
    14761 atggggggtt tatccggcaa atgaaccgcg tcgtttactc aacgcctttc gtgtggccgc
    14821 agagggggag ttctgcaatg cacaagatga gcccattgac ctgcctgcgg atgctctgat
    14881 tggcattgcc cacccgttag aaatggcagc agaaatgcgc agtgaatttg cacagctttt
    14941 tgccgattac gaaattatgc cgccttttcg ccagttggcg cggcgcacgg tgctgctcac
    15001 acctgacgag tcagccagta acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg
    15061 gcaacttatg ggaatgcgat acaaaggctg ggagtcaggc tatgaggacg catttgtcta
    15121 tgacttaggt gcgtaccggc tggtccttaa gttttcaccc ggttttaacc actacaatgt
    15181 tgatagcaaa gcgctaatga gcttccgttc tcttcgggtt taccgtgaca ataaatccgt
    15241 cacttttgcc gaacttgatg tgtttgattt gagtgaggca ttaagcgcac ctgacgtcat
    15301 tttccattaa aacacagcgg gcagtgatgc aactgcccgt tatcaacacg acctaccagc
    15361 gggggaagcg atgatcgtgc aaaaagagct ggttgctatt tacgattatg agatccctgt
    15421 acctgaagag ccgttttcct tcagacttga aatccataaa tgctctgaat tatttacagg
    15481 ttccgtctat cgactggagc gattccggct acatccaaca tttcatcaac gtgatcgaga
    15541 agatgccgac cactaataaa tgatgcgttg atttatataa gagatgagtt tattgatgag
    15601 cggaaattac gaggtgaatc acatgaaaca gtaatagcaa tttttaattg tgaactacag
    15661 aatatattca accaagaaat agaataatat actctagata attcaagtcg caggaaggcg
    15721 ataagcgagt gagtccccag gagcttacta aagtaagtga caggggtgaa cgaacgcagt
    15781 cgcagcacat gcaacttgaa gtatggcgag tatagcttta ttaaaggggg aaacggtatt
    15841 cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac cgtaacagag
    15901 ccactaatag ctccagtaca gcaatgagca cggttaactt ttgtgtcagc gtgtccatcc
    15961 gctctccttt taaggagcgc aacaccagca ccttaccctt acactgcctg tcgatactgg
    16021 tttacgtatg ttggaccacg atgttgcact ccggatcagg caccacctgc agcaccaccc
    16081 gtccggtagc caggacttga aattggttcg atccggcgca acctcccaac agtcaggatt
    16141 ataaatctca ttatcttaat tgcaacgggg tccagcggtg gttataattc gttcgttgga
    16201 acacgatgtt gcacaggctg cggtgttagg cctgaaagta ataaataaaa gaacctgcct
    16261 caccagcagg tttttttatt ttttgcgatc tgctttccag atattttccg ctcaaacaac
    16321 taatgcgcca aacatttatt gcgcgtaaaa tatcgtttat ttcattaata catttcaggg
    16381 atgaatatat gtcaccgcag aataatcacc tgcagcgtcc gcctgctgct gtgttatacg
    16441 ccgttgaact ggcaaaatta aaacaaaatg ataacgcacc ttgcccgccc ggttggcagt
    16501 taagtttgcc tgcggcccgt gcttttattc ttggcgacga agcgcaaaat attcgccgta
    16561 aagtggtgat tagtccctct gctgtcgaac gcatgttagt cactcttgcc accgggcgcg
    16621 gtttgatgtt ggtgggggaa ccgggtaccg caaaatctct cctgtctgaa ttactggcaa
    16681 ccgctattag cggcgatgcc gggttaacca tccagggcgg cgcatctacc accgaagatc
    16741 aaatcaagta cggctggaac tacgccctgc ttatcaatca tggtccgtca acggaagcgc
    16801 tggtccctgc cccgctttac ctgggaatgc gcgacggcaa aatcgtccgt tttgaagaga
    16861 taacgcgtac tccactggaa gtacaagact gtttattagg tatgctttct gacagggtta
    16921 tgacggtgcc ggaactcact ggtgaagcca gtcaactcta tgctcgggaa ggctttaata
    16981 ttattgccac tgccaatacg cgcgatcgcg gcgtcaatga aatgagtgcc gcgctaaagc
    17041 gtcgcttcga ttttgaaact gtctttccga ttatggattt cgcccaggag ctggagcttg
    17101 tcgccagtgc ttcggcgcgt ttgctggccc atagcggcat tccgcataaa gtgcctgacg
    17161 cggtactgga gttactggtc cgcacctttc gcgatctgcg tgccaacggc gaaaagaaaa
    17221 cgtcaatgga tacactgaca gcgattatgt ccaccgctga agccgtgaac gtggcccacg
    17281 cggtgggcgt ccgcgcctgg tttttagcga atcgagcagg tgagccagcc gatctggtgg
    17341 actgtattgc cggaaccatc gtcaaagata acgaagaaga ccgcgcgcgc ctgcgccgtt
    17401 acttcgaaca acgtgttgcc acacataaag aagctcactg gcaggcttat tatcaagctc
    17461 gccaccgcct gccgtgagga aagatgcatg agcgagccgt taattgtcgg catccggcat
    17521 catagcccgg cctgcgcccg gctggtgaaa tcgttaatcg aaagccagcg gccacgatac
    17581 gtattgattg aaggcccggc tgattttaat gaccgggtag acgaactgtt tttagcccac
    17641 cagcttccgg tagctattta cagttattgc cagtatcagg acggtgcggc ccccgggcgt
    17701 ggtgcctgga cgccatttgc tgaattttcg ccggagtggc aggcgctaca agccgcacgt
    17761 cgtattcagg cacaaactta cttcatcgat ttgccttgct gggcgcagag tgaagaagag
    17821 gacgattcgc ctgatacaca agatgaaagc cagaccttac tgctgcgtgc cacccgcatg
    17881 gataacagcg ataccctgtg ggatcactta ttcgaagatg aaagccagca aactgcatta
    17941 ccctctacgc tggcgcacta ttttgcccaa ctgcggggcg attcccccgg agatgctctc
    18001 aatcgtcagc gcgaagcctt tatggctcgc tggattacat gggcgatgca gcaaaacaat
    18061 ggcgacgtgt tagtcgtctg cggcggctgg cacgctcctg cactggcaaa aatgtggcgc
    18121 aaatgcccgc aggaaatgaa caagccagaa ttgtcctcgc tggcagatgg cgttacaggt
    18181 tgttatctca caccctacag tgaaaagcgc cttgatgtgc tggcaggata cctttccgga
    18241 atgcctgccc cggtctggca aaactggtgc tggcagtgtg gcttacagca ggcaggtgaa
    18301 caactgctga aaacggttct tacccgtttg cgccagcaca aattgcccgc ttctaccgct
    18361 gatatggctg ctgctcatct gcatgcgatg gcactggcac agttgcgcgg ccatacacta
    18421 cctctacgca ctgactggct ggatgccata gctggctcgc tgattaaaga agccctgaat
    18481 gcgccgttgc cgtggagcta tcgcggagtt attcatcccg ataccgatcc gattttgcta
    18541 acggtgatag acacattagc gggtgacgga ttcggtaaac tttccccttc tacgccacaa
    18601 ccgcctctgc caaaagatgt cacctgcgaa ctggaacgta ccggaatctc tcttccggcg
    18661 gagcttacct taaatcgctt tacccccgat ggactggcgc aaagtcaggt gttacatcgg
    18721 ttggcaatac tggagatccc aggggttgtg cgccagcagg gaagtacgct gacacttgca
    18781 ggcaacggtg aagaatgctg gaaattaacc cgcccgctta gccagcatgc ggcattgatt
    18841 gaggccgcct gctttggtgc cacactccag gaagccgcac gccataaatt agaagccgat
    18901 atgctggacg cgggtggaat cggctgtatc accacatgtc ttagccaggc agcgttagcg
    18961 ggtctggcgt ctttcagtca gcaattactg gaacaactca cattattaat tgcccaggaa
    19021 aatcaattcg ccgaaatggg ccaggcgctg gaagtgctat atgccttatg gcggctggat
    19081 gaaattagcg gtatgcaagg cgcgcagata ttacaaacga cattatgcgc ggccatcgat
    19141 cgcacgctgt ggctatgtga atccaacggc agaccggatg aaaaggagtt tcacgctcac
    19201 ctgcatagct ggcaggcgct ttgccatatc ctgcgcgatc tacatagcgg cgttaattta
    19261 cccggcgttt cgctttctgc ggcggtagcc ttactggagc gccgcagtca ggcgattcat
    19321 gccccggcgc tggatcgcgg cgcgactctt ggcgcactga tgcgtctgga acatcccaac
    19381 gccagtgccg aagcggcgct gacgatgctg gcgcagttat ccccggcaca atccggcgag
    19441 gcgctgcacg gtttgctggc attagcccgt catcaactgg cctgtcagcc ggtatttatc
    19501 gccggtttca gcagtcattt aaatcaactg agtgatgccg attttatcaa tgccctgccc
    19561 gatttacgcg cggcgatggc ctggctacca ccacgagaac gcgggacgct ggcgcatcag
    19621 gtgcttgagc attaccagct cacacagctt cccgtttcag cgctgcaaat gctgttgcat
    19681 tgtccaccac aagccattgc acatcatcaa caactcgaac agcaggcact ggcatcgctg
    19741 caacactggg gagttttcca tgtctgaact gaacgatctt ctgaccaccc gtgagctaca
    19801 acgctggcga ttaattcttg gcgaagcggc agaaacgacc ctttgtgggc tggatgacaa
    19861 cgcccggcag atagaccacg cgctggagtg gctgtatggg cgcgatcctg aacggctcca
    19921 gcgtggtgaa cgttacggtg gattaggtcg ttcaaatctc accacccctg agtggattaa
    19981 cagcattcac acgctgtttc cgcaacaggt gattgagcgg ctggaaagcg atgccgtgct
    20041 gcgctacggc attgaagatg tggtgacgaa tctcgacgtg ctggaacgta tgcagccttc
    20101 tgaaagcctg ctacgcgccg ttttgcacac caaacatctg atgaatcccg aagtactggc
    20161 tgccgcccgc cggatagtgc accaggttgt ggaagaaatt atggctcgac tggcaaagga
    20221 agttcgtcag gctttttctg gagtacgcga tcgccgtcgc cgctcattta tttcactggc
    20281 gcgaaacttt gatttcaaaa gtactctgcg cgccaacctg caacactggc acccgcaaca
    20341 cggcaagttg tatatcgaat ccccccgctt taacagccgc ataaaacgct atagcgaaca
    20401 atggcaactg gtcctactgg ttgatcaaag cggatcgatg gttgattcgg tgatccactc
    20461 cgcagtgatg gcggcctgtt tatggcagtt acccggcatt cgtacccatc tggtcgcttt
    20521 tgacaccagc gtggttgatc tcacggcaga cgttgccgat ccggtagagc tattaatgaa
    20581 agtgcaatta ggcggcggga ccaatatcgc cagtgctgtg gaatatggtc ggcaacttat
    20641 tgaacaacca gcgaaaagcg tcattatcct cgtgagcgat ttttacgaag gggggtcatc
    20701 atcattgctg acgcatcagg tgaaaaagtg tgtccagagc ggcatcaaag tgctgggact
    20761 ggcggcgctc gatagcagcg caacaccttg ctatgaccac gatacggccc aggcgctggt
    20821 taatgtcggc gcacaaatag ccgcaatgac accgggcgag ctggcttcct ggcttgcgga
    20881 gaatcttcag tcatgaattc actacgtccg gaattattag aactgacgcc gcaggctctg
    20941 acggcgttaa gcaatgccgg ttttgttaag cgcagcctta aggaactgga aaatggcaac
    21001 gtcccggaaa tcagtcatga gaacggcgct ttaatcgcca ccttcagtga cggtgtccgt
    21061 acccagctgg cgaacggcca ggcactgaaa gaggctcagt gcacctgcgg ggccagcggc
    21121 atgtgccgtc atcgcgtgat gctggtgtta agttatcaac gactttgtgc caccgcccag
    21181 cctacggaaa aaaaagaaga agagtgggat ccagcaatct ggctgaaaga actggctaac
    21241 ctgcccgatg ccacccgcaa acgcgcgcag gcgctggtcg ctaaaggcat caccattgag
    21301 ttgttctgtg cgcctggcga aattccctct gcccgattac cgatgagcga tgtgcgtttt
    21361 tattcccgca gcagcattcg cttcgcacgc tgtgattgca ttgaaggcac actttgcgaa
    21421 catgtcgtac tggctgtgca ggccttcgtc gaggccaaaa ctcagcaagc ggaatttact
    21481 catttaatct ggcagatgcg cagtgaacac gtgacatcat ctgatgatcc gtttgccagc
    21541 gaagaaggca aaacgtgtcg tcaatatgtt cagcaattaa gccaggcatt atggctgggc
    21601 gggataagtc aaccgccaat ccattacgag gccgctttca gtcgcgcgca gcaagcggcg
    21661 gaacgctgca actggcgatg ggtgagtgaa tcgctacggc aattgcgcgc cagtgttgat
    21721 gccttccacg cccgtgccag ccactaccat gccggagaat gcttacgtca gcttgcggca
    21781 ttaaacagtc gattaaattg cgtacaagag atggcgcgac gcgacagtat cggtgaagtt
    21841 cctcccatgc cgtggcgcac ggtcgttggc gctggcattg ccggagaagc aaaacttgat
    21901 catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca gtatggttta
    21961 cgcatctggt ttaccgatcc cgacacagga agtattttgc atctttcgcg tagttggcag
    22021 agaagcgaac aggaaaactc accggcagcg acgcgtcggc tgtttagttt tcaggctggc
    22081 gcactggcag gtgggcaaat tgtttcacaa gctgcaaaac gcagtgccga tggcgagctg
    22141 ctgttagcga cccgtaaccg cttgagtagt gttgttccac tgtcgccaga cgcctggcaa
    22201 atgttgagcg cgccgttgcg ccagccgggc attgtggctt tgcgggaata tttacgccag
    22261 cgtccccccg cctgcatacg accactcaac caggtcgata acttatttat tctgccggta
    22321 gcggagtgta tttcgctcgg ttgggatagc agccgccaga cactggatgc gcaggtaatc
    22381 agcggcgaag gagaagataa tgtgctgacg ttatcccttc cggcatctgc ctgttctcct
    22441 tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt gagtctggtt
    22501 tctggctttg tcagttttgt agaggggcaa ttgacactgg aaccacgggt gatgatgaca
    22561 aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct accttctgcc
    22621 agcgttctgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca ggcgttactt
    22681 attcaactgc tccataacgg ctggcgctat caggaacaga gtgctattgg tgaggcagag
    22741 ttgctggcga atgacctcac cgcggtgggt ttttatcggc tggcacatgt gttgggacaa
    22801 tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt tttgctttgc
    22861 gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttttta ttaagagttc
    22921 tactcctgtt ccggactgct cacgccacag tattaggcat atcctatata gcccctggtg
    22981 agagtcacca gttccttgat taaataaaat ggagttttac atgaaggctt tcaataagct
    23041 gttttccctt gttgttgcat ctgttctggt tttctctctt gctggctgcg gtgacaaaga
    23101 agaatcaaag aaattcagcg ccaatctgaa cggcactgaa attgccatta cctatgtcta
    23161 caaaggtgac aaggtgctta agcaatcttc tgaaaccaaa attcaatttg cctccattgg
    23221 tgcaaccacc aaagaagatg ccgccaggac acttgagccg ttaagcgcca aatacaaaaa
    23281 catcgcgggt gttgaagaaa aattaaccta taccgatacc tacgcgcagg aaaacgtgac
    23341 tattgatatg gaaaaagtgg attttaaagc cctgcagggt atttcaggaa tcaatgtttc
    23401 tgctgaagat gccaaaaaag gtatcactat ggcgcaaatg gaactggtga tgaaagccgc
    23461 tggttttaaa gaagtgaaat aatctgtcgg cggccatgtt tcgcatggcc gccatacccg
    23521 tcttagcttt tcttcacatg ctggcgcgct gccagtccac gcagaaaata acgtaaaaat
    23581 tgatcgccgc attcgcggaa gtttttatga tccggtgcac gcatcatcgc cgtaatttcc
    23641 ggcatcgaaa cgcggaactg ctgttcggtg aggattgcca gaatgtcatc ggttttaagc
    23701 gaaaacgcga tgcgtaattt tttcagcacg atgttgttat taatgcgacg ttccggctcc
    23761 agtgccggag cagactcatc cttgccgcgt ttttcataaa tcaggccatt gaggaatgaa
    23821 gacaaaacaa tgtccggaca acgctgaaaa ccctcttcgt cttctttacg tagccagacg
    23881 gcgatctgtt ccgcggtggc ttcgacatta cccagcgcca gaatacgcac caggtcatta
    23941 ttattggctt tcaaaatgta gcgcacgctg cgcagaatat cgttacttag catgaggcct
    24001 tcaggtgttg atgaggcaaa aagccatttt agcagtcttt tacaggccaa tcgcctcttt
    24061 taagcttttc agataacggc ggctgaccgg cacggttaag ccattacgca aaatcaactc
    24121 ggcctggccg ttatcttcca gacggatctc ctgtaaatgc gcgaggttaa ccagatactg
    24181 acgatggcag cgcagtaatg gtgtacgact ttccagggta cgtaatgtca gttcggtaaa
    24241 gccctctttc ccttcgtgac tggtaacgta gacaccgctc atccgactgc tgacaaatgc
    24301 cacatctttc atttgcagca aataaatccg actatgcccc gtacaaggga taaatttcag
    24361 cgcctgttga ttttccggta acagcgaaac atcctgcttg ctgcgctcct gacgcaatcg
    24421 cgccagcgtt ttctccagtc gcgcttcatc aattggcttc agcagataat caaaggcatg
    24481 ttcttcaaag gctttaatgg cgtattcgtc aaacgcggtg agaaaaacaa tatacgggcg
    24541 atgttccggg tcaagcatcc ccaccatttc cagaccactg atgcgcggca tctggatatc
    24601 gagaaacagc acatccgggc gcagtttatg caccgcgccg atcccttcca cggcgtttga
    24661 acactctcca acgatttcaa tatcgctctg ctcctgcaaa aatacgcgca ggttctcccg
    24721 tgcaagcggt tcatcatcga caattaagac tttaatcatg cctcgtccct ccatggtagt
    24781 cgtaacgtta ttcgggtgta actatcaggc tcacaggcga cgcttatccc atagtcatcg
    24841 ccaaaccgtt cacgtaaacg cttatccacc agattcatcc ccagcccact ggcattggtt
    24901 accggttgat acaaaccggc attgtcttcg atctccagca tcaaatgttg cccctcacgt
    24961 cgggcgctga ttgccactcg ccctgtatcc agcagttgtg atgtcccatg tttaatggcg
    25021 ttttccacta tcggttgcag ggtaaacgcg ggcaattgct gctgggataa ttcttgcgga
    25081 atagcaatgt tgacctgcaa ccgcgactgg aagcgcgcct tttcaatttg cagataagcg
    25141 ttcacatgtt caatttcgtc ggcgagagta acaaactccg aaggccgctt taagtttttg
    25201 cggaaaaaag tggaaagata ctgcaccagc tggctggcct gttcgctgtc gcggcggatc
    25261 accgctttaa tggtgttaag cgcattaaac aaaaaatggg gattcacctg ggcgtgaagc
    25321 agtttgatct ctgactgggt gagcatcgct ttttgccgct catattgccc ggcgaggatc
    25381 tgcgccgaaa gcagttgcgc aatcccctcg cccagcgtgc ggttgattga actgaataaa
    25441 cggtttttgg cttcatacaa tttgatggta cccatcactc gctgattttc accacgcaac
    25501 ggaatgacca gcgtcgaccc cagtttgcat tgcggatgca aagagcaacg gtaaggcact
    25561 tcgttgccat cagcgtagac cacttcaccg gtttcaatcg cttttaaggt ataagtcgaa
    25621 gaaatcggtt tgccgggtaa atggtggtcg tcaccaattc cggtaaaggc cagcaatttc
    25681 tctcgatcgg taatcgcgac tgcaccaata tccagctcct gatacagcac ctgtgccact
    25741 ttcatgctgt tcacttcgtt aaacccctgg cgcaaaatgc cttccgtcga ggccgccact
    25801 ttcagcgcag tggcagaaaa agccgaagtg tatttttcaa acatcgcgcg tttatcgagc
    25861 aatatacgca taaacaacgc cgcgccgacg gtattggtga ccatcattgg cgcagcaata
    25921 ttactcacca gacgcaccgc atcttcataa ggtcgggcga tcgccaggat gatcaacatt
    25981 tgcaccattt cagcgacgaa cgtgacggca ccggcggtaa tggggttaaa gactttatca
    26041 gtgcgcccgc gacggatcag gatgctgtga accaggccgc cgagcaatcc ttcaacgatg
    26101 gtcgagatca tgcaacttag cgcggtcatg ccccccatcg aatatcgatg taacccgccg
    26161 gtcagaccaa ccagcccacc gacgaccgga ccgccgagta agccgcccat taccgcgcct
    26221 atcgcacggg tattggcaat agaatcgtca atgtgcaacc caaaccaggt gcccatgatg
    26281 cagaagatgg aaaagacgat gtagcagaga aatttatgcg gcagacgaac cgtgacctgc
    26341 attaacggta tgaataatgg cgttttactc attaaccacg caatgactaa aaaaacgcac
    26401 atctgctgaa gcagcagcaa caccagatta aaatcgtaca tacccgcaaa ccacacttcc
    26461 ctttaaaatg cgtaacatac attgcttgcg tttaactttc tttgaactct tgcagaaaaa
    26521 tgagaattcg tgagtacgat cactcaaaat cgcctggcaa aaataaaatc accctataga
    26581 tgcacaaaaa acgggcaaaa ctacctggtt cgcaaaactg cgtctaaagt taaaccggga
    26641 cctcgcgagc aagggtgaga cgatggcgct ttacacaatt ggtgaagtgg cgttgctttg
    26701 tgatattaac cctgtcacgt tacgcgcgtg gcagaggcgt tacggattgc tgaaaccgca
    26761 acggacagac ggcggccatc ggctgttcaa cgatgccgat attgaccgga tccgcgagat
    26821 caaacgctgg atcgacaacg gcgtgcaggt cagcaaagta aaaatgctgc tcagtaatga
    26881 aaatgttgat gtgcagaacg gctggcgcga tcagcaagaa acattactga cttacctgca
    26941 aagcggcaat ctgcatagcc tgcgaacgtg gatcaaagag cgcggtcagg attaccccgc
    27001 ccagacactc accacacatc tgtttattcc tctgcgccga cggcttcagt gccaacaacc
    27061 gactctccag acgctgctgg cgatcctcga cggcgtactg atcaactaca tctctatttg
    27121 cctggcttcg gcgcgcaaaa aacagggtaa agatgcactg gtggttggct ggaatattca
    27181 ggataccact cgtctgtggc tggagggctg gattgccagt caacaaggat ggcgcattga
    27241 tgtcctcgcc cactcgctca atcaactacg ccctgaactg ttcgaaggcc gtacattgct
    27301 ggtgtggtgc ggtgataatc gaacctccgc ccaacagcag caactcacca gttggcaaga
    27361 acaaggccat gatattttcc cactcggcat ttaatgattc gttaacaaat gcgctttact
    27421 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
    27481 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
    27541 tttattttgc gtgctggctg aacgcctgca ccagcctttc aaacaggcgg tctgcgatga
    27601 tcgccgccag tgccaccagt aacgctccct ggatcacata cgcggtatta aatccgctaa
    27661 gtccgatgat gatgggcgta cccagcgtgc tggcccctac cgttgaggcg atcgtcgccg
    27721 taccaatgtt gataatcacc gaagttcgca cgccagccag aatcaccgga gccgccagcg
    27781 gtagctcgac cttacgcagt cgctgaccac gactcattcc catacctttc gcaacttctg
    27841 tcacgctggc atcaatcgct cccagcccgg caagtgtcgc ctgcaggacg ggcagcacgc
    27901 cgtaaaggat caaggcgata atcgctggtt tcagaccaaa gccgatcacc ggaacggcaa
    27961 tcgccagcac cgcgacgggc ggaaaagtct gcccaacggc ggcaatagtt tccaccagtg
    28021 ggcgaaattc cgcaccccac gggcgagtga cagcaattcc ggcaccagta ccaatgatca
    28081 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
    28141 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
    28201 tataaggtag ccagaaaatc agtgccacaa acaaagcaat gagccagaac agcggatcgc
    28261 gcaacatctt catacgctta cgcctcctcc agcagatcct gaaaatgcag cgtgccgcaa
    28321 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagagaa
    28381 agcgcatcgc gtagcgtcat cctttcttcc agtgcctcac cttcagcccg ttcttcacgg
    28441 cgcacgtaat ctgccacgct acgtaacgaa agcaggcgta cacccagctc actacgacca
    28501 aaaaactggc ggacaaaatc attcgccgga tgagtcagca tcgccagagg attgccctgc
    28561 tgcacgactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
    28621 atatcatgag tgaccagcac aatggtacgc ccctgcaaac ggtgaatgcg cgtcatctct
    28681 tgttgcaacg cgccgcgcgt taccgggtcc agcgcgccaa aaggttcatc catcagtaag
    28741 acttgcggat cggcagccag tgcgcgcgcc actcccacac gttgctgctg accaccggaa
    28801 agctgatgcg gataacgctc acgcaaattt ggctccagcc ccagtagcgc cattaattcg
    28861 tcgatacgat cgtcgatccg cgcccgtgac catttttgca attgcggcac ggtggcgatg
    28921 ttttgtgcca cgctccagtg gggaaacagg ccaatagatt gaatggcata gcccatccgg
    28981 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcaccccg
    29041 ctgtcatgct ccaccaggcg gttaatcatt ttcagagtgg tggatttacc ggagcctgat
    29101 gtaccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
    29161 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
    29221 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
    29281 cacccccagc aacactaaat caatggcact gcttagcagc ccctggaaaa ccagcgcacc
    29341 aaaaccgcct gcgccgatta acgccgcaat caccgccatg ccaacggttt gcaccatcac
    29401 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
    29461 ctgcaccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
    29521 caagccgact actacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaataag
    29581 tgcgggtgtc attccggttc ctgctatgcc gagcgttccc agccacggaa aggccgtaac
    29641 cagcgcggca agcggcgcaa tcagcaggcc aaagagcgct accgaaggca cggtctgaat
    29701 gacattcagc agagaaaaaa tcgccccctg ccgcgcggtg gaaaagtagc accagatgcc
    29761 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
    29821 tgccagagcg tcgtcaaaca catcctgacg gttggcgtat tctttcatta acgagagatc
    29881 gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca tatgcaacaa
    29941 ccagcgccac agcggatgcg tggagattcg gcggatggca tcgctacagg ccagcagtgc
    30001 cagcgccgca gccagccaga aaccactgcc gaggctggta cgtgccagcg cactgccatt
    30061 ttgcgccagt tgtgtcgccg cctttccagc tccccacacc agcaatacga agacgaattg
    30121 cgccagaatg agtgcaaaaa tgctgccttt tttagcggga ataaaacagg ctgtcagcca
    30181 ggcgcaacca acgcccacca gcatccagag agtttgcggc cacagctgcc agagatgacg
    30241 cccctcaccc gaaactaaac gattaggcgc gtaactgata aacggcagcg ccgctgcgat
    30301 tgccgtcagc aacagcagca gcgccagaac aggattaata cgtagataag tcacgggaaa
    30361 ttacttcgtc cacccttttt gtttcaggta gtcggcagcc acttttttgg catccagtcc
    30421 ttccacagca atgctggcat tcagttgctg caatgttttt tcatcgaggc tggcgaagac
    30481 tggctgtagc cactgtgcca tttgtggata ctctttcagc accgactcac gcaccactgg
    30541 tgcaggcgcg tagataggtt gcacgccttg cggatcgctt aaggtttgca gccccagcgc
    30601 cgcgaccggg ccgtcagtgc cgtaagccat tgcggcatta acgccagagg tttgctgggc
    30661 agcggctttg atcgttaccg ccgtgtcgcc accagccagt gacagcaact gatcctgacc
    30721 gagcttaaag ccataggctt tttcaaacgc gggtaaggca tcggcgcgtt caataaactc
    30781 tgccgaggct gccagtttga aggtgccgcc ctcttgcaga taacgactca ggtcggcaag
    30841 cgaagtgagc ttgtttttct ctgccacatc ctgacgcacg gcgatggtcc aggtgttatt
    30901 tgcaggtgcg ggcgtcagcc agattaactt gttttgctct gcatcgagtt ttttgacttt
    30961 ctcgtaacct tgctgcacgt ttttccacgc tgcatcgttt tcatctttaa agaagaacgc
    31021 cccattgccg gtatattccg gatagatatc cagctcgcca gaagtgatcg cgccgcgcac
    31081 cacaggagtc gttccaagtt gcactttatt taccgtagga acgccgtgac tttccagcac
    31141 ctgcaaaatg atattgccga gcagcgcgcc ttccgtatcg atttttgaac caaccttaac
    31201 gggggaagct gcctgtagcg gcaggctcac ggctgccaac aaaaccagtg aacctgccca
    31261 gacctttgag agtggcatga tgctttcctc attcttttac tgttgttttc agcgaattaa
    31321 gagaaaagca tagttgataa tgacagggtt agcctgtctg ggtggattca gttgcagaat
    31381 cagataaata cttaaaaggc atattttgcc cggcgtttct tgccggatgc ggcgtaaacg
    31441 ccttatccgg cctacaaaca tcgaaaatta caacaactca aactcggctt tcttaacgcg
    31501 tgcggaatca gtgccgataa agacattgaa cttgccaggc tcggcgtcat atttcatctg
    31561 ttgattccag aacttcagcg cctcaatatc aatcgggaag ctgacggtct gagtttcgcc
    31621 cggtttcaga gtgattttct caaagccttt cagctgcttc accgggcgac tcatggaagc
    31681 cgtcacatcc tgcaagtaca tctgtactac cgtggcccct tcgcgtttac cggtgttcgt
    31741 cacctgcacg ctggccgtca ctttgccgtc acgcttcatg gtcggcgcag aaagtttcac
    31801 atcagagacg gtgaaagtgg tgtagctcag cccatagccg aacggataaa gcgccccgtt
    31861 agcttcatca aaataacgcg aagtgtattt gttcggcttg tcggcattat acggacgccc
    31921 ggtattcaga tggctgtagt acaccgggat ctgcccgaca gaacgcggga aggacatcgg
    31981 cagcttgccg gacgggttat aatcgccaaa caatacatcg gcaattgcat taccgccttc
    32041 agtccccgca aaccaggttt ccagaatcgc atcagcctgc tgatcttctt tcaccagcgc
    32101 cagcggacgc ccattcatca gcaccagcac cagcggttta ccggtggctt tcagcgccgc
    32161 aatcaagtca cgttggcttt gcggaatagt gatatcggta cgactggaag cttcgtgcgc
    32221 catcccctga gcttcaccga ccacagccac caccacatca gattgtttcg cggtttgcac
    32281 cgcttcatca atcatctctt gcggcgaacg cggatcgact ttgaccgctt cttcatactg
    32341 attcaggaaa tcgataatgc ctttgtcact ggtaacgttc gcccctttgg catacagcac
    32401 tttaccgttt tcaccgacgg cgtttttaat cccggttagc acagtaacag attgatcggc
    32461 gacacctgct gccgaccagc ttcccatcac gtcacgcttg ctgtctgcca gcgggccaac
    32521 caccgcaatg gttgcagatt ttttcagcgg cagcgtttcg agacggtttt tcagcaacac
    32581 caggctttcg cgcgccactt cacgcgcttc tttgcggtgc aggcggcttt cggcattggt
    32641 atccaccggg tcagactctt tcggccccaa atggctgtat ggatcgttaa acaatcccat
    32701 atcatattta acgttcagta catggcgggc agcatcgtcc agctcttcca tcgtcacttt
    32761 gccggatttg atcaacccag gcagatactt cgagtaatac tcgtcgctca tgctcatgtt
    32821 gattccggac ttcagcgcca cgcgtaccgc atcctctggg tctgccgccg tgccatgttt
    32881 aatcagttct ttgattgcgc cgtgatcgga aacggtgatg cctttaaagc cccactggtc
    32941 gcgcagaaca tctttcagca gccaggagtc ggaggtggct ggcgtgccgt tcagcgagtt
    33001 cagcgccacc atcaccgcac cgctgcctgc atccagcccc gctttgtacg gcggcatata
    33061 gtcgttaaac aggcgctgcg gactcatatc gacggtgttg tactctttac cgccttctac
    33121 cgcgccgtat gcggcaaagt gtttgacgct ggtcatcacc gagtagcgat ctgccgggct
    33181 tttaccctgc atcgcttcca ccatggtttt acccattatt gaggtgagat acgtatcttc
    33241 gccaaaacct tcggaagcac gtccccagcg cggatcgcgc gagacatcga ccatcggtgc
    33301 ccaggtcata ttcaggccat catctgccgc ttcataagca gagacacgcc cgactgtttt
    33361 caccgcatcg aggttaaaag acgaggccag accgagacta atcgggaaca ccgtgcgctg
    33421 accgtgcagc acgtcgtaag caaagaaaag aggaattttc aggcggctta attccatcac
    33481 ctgatcctgc atggcgcgga tatcctgacg ggttacggtg ttgaaaatcg ccccaacctg
    33541 accgtctttg atcatctcgc ggatcgcctc tttcgggtta tccgggccga cgctgattaa
    33601 gcgcagctga ccaattttct catcaactgt cattttctta agcagttcgg tgacgaacgc
    33661 atcccgcgct tcgggcgtta atggatggtt gccgaataaa tcatccgcca gtgctggctg
    33721 cagggccaga ctcaccgcga ttcctactga acatagccat ttcatatgga tttttcttcc
    33781 tttgttgccc gacgtggcag cgaaaatggt gcaaaaaccg tagtttgcca taagcatgat
    33841 ggagagagaa aaagaatgct cagtttattg tctgaatttt caaaatattc actcgctgaa
    33901 ttgtcataca aggcgctatt ctagtttgtg atattttttc gccaccacaa ggagtggaaa
    33961 atgtcttcca tgacaacaac tgataataaa gcctttttga atgaactttc ccgtctggtc
    34021 ggtcattcac acctgctcac cgatcccgca aaaacggccc gctatcgcaa gggcttccgt
    34081 tctggtcagg gcgacgcgct ggctgtcgtt ttccctggct cactactgga attgtggcgg
    34141 gtgctgaaag cctgcgtcac cgctgacaaa attattctga tgcaggcggc caatacaggc
    34201 ctgaccgaag gatcgacgcc aaacggtaac gattatgatc gcgatatcgt gatcatcagc
    34261 accctgcgtc tcgacaagct gcacgtactc ggtaagggcg aacaggtgct ggcctatccg
    34321 ggcaccacgc tctattcact ggaaaaagcc ctcaaaccgc tgggacgcga accgcactca
    34381 gtgattggat catcgtgtat aggcgcatcg gtcatcggcg gtatttgtaa caactccggc
    34441 ggctcgctgg tgcaacgtgg cccggcgtat accgaaatgt cgttattcgc gcgtataaat
    34501 gaagatggca aactgacgct ggtgaaccat ctggggattg atctgggcga aacgccggag
    34561 cagatcctta gcaagctgga tgacgatcgc atcaaagatg acgatgtgcg ccacgatggt
    34621 cgtcacgccc acgattatga ctatgtccac cgcgttcgtg atattgaagc cgacacgccc
    34681 gcacgttata acgctgatcc ggatcggtta tttgaatctt ctggttgcgc agggaagctg
    34741 gccgtctttg cagtacgtct tgataccttc gaagcggaaa aaaatcagca ggtgttttat
    34801 atcggcacca accagccgga agtgctgacc gaaatccgcc gtcatattct ggctaatttc
    34861 gaaaatctgc cggttgccgg ggaatatatg caccgggata tctacgatat tgcggaaaaa
    34921 tacggcaaag acaccttcct gatgattgat aagttaggca ccgacaagat gccgttcttc
    34981 tttaatctca agggacgcac cgatgcgatg ctggagaaag tgaaattctt ccgtccgcat
    35041 tttaccgacc gtgcaatgca aaaattcggt cacctgttcc ccagccattt accgccgcgc
    35101 atgaaaaact ggcgcgataa atacgagcat catctgctgt taaaaatggc gggcgatggc
    35161 gtcggtgaag ccaaatcgtg gctggtggat tatttcaaac aggccgaggg cgatttcttt
    35221 gtctgtacgc cggaggaagg cagcaaagcg tttttacacc gtttcgccgc tgcgggcgca
    35281 gcaattcgtt atcaggctgt gcattccgat gaagtcgaag acattctggc gctggatatc
    35341 gctctgcggc gtaacgacac cgaatggtat gagcatttac cgccggagat cgacagccag
    35401 ctggtgcaca agctctatta cggccatttt atgtgctatg tcttccatca ggattacatc
    35461 gtgaaaaaag gcgtggatgt gcatgcgttg aaagaacaga tgctggaact gcttcagcag
    35521 cgtggcgcgc aataccctgc cgaacataac gtcggtcatt tgtataaagc accggagacg
    35581 ttgcagaagt tttatcgcga gaacgatccg accaacagta tgaatccggg gatcggtaaa
    35641 accagtaagc ggaaaaactg gcaggaagtg gagtaataat tacggatggc agagtatcgc
    35701 catccgtcat tcatttaatc gttctgtgcc gtctgccccg ccgccgccat ttgggcggct
    35761 ttttgttttt tatagctcaa cgctgctgcc ggaacaggca tcactttacc ggtttcaatc
    35821 caggtacgca ggcggctggc atcggcaaaa tgggtatatt tgccaaacgc gtccatcact
    35881 accagcgcca ccggtttatt attgataacc gtgcgcatca ccagacaatg gcccgccgca
    35941 ttggtaaaac cggttttggt taactgaata ttccagttat cacgatacac cagatggtta
    36001 gtattgcgga acggcagcgt atacgtcggg ttagagaagg ttgccatatc ttcccgggta
    36061 gtacttaact gcccgatcaa cggatattgt ttgctggcaa tgagtagttt ggttaagtca
    36121 cgggcggttg aaacgttatg caccgacaat ccggttggtt caacaaagcg cgtgttgttc
    36181 attccgagcg atttcgcttt cgcattcatc gccttaataa aggctttgta accaccggga
    36241 taatggtgcg caaggcttgc cgccgcgcgg ttttctgaag acatcagcgc cagcaacagc
    36301 atatctttac ggctgatttc gctgttcagt cgtacgcgcg aatagacccc tttcatctcc
    36361 ggcgtctggc tgatatccac ttttagtttt tcatccagcg gcagtcgtgc atccagcaca
    36421 accatcgcgg tcattaattt gctgatagac gcaatcggac gcaccagatc cgggtggttc
    36481 gaatagatca ctttgttggt attcagatca acaatcatcg cgctaccgga ggcaatttcc
    36541 ggttgtgacg cggtggtagc ggctgccgtt ttcgcaacgg cctgcggtgc aaaaggcaca
    36601 gccagcatca gggccaggct aaataaagaa actcgaaatt tcggcatgat gagcattcag
    36661 atagtggttc acgcgcacgg gttgcgcacc gccggagtaa ggatttactg aggctagcga
    36721 cgccatcata acgagcaaaa agtgcgatcg tcaaaggaga atcgtgagga aatgctgcat
    36781 tgctgacatt tacgccagca atgcagcgca aaagaacttt ctagaacaaa cgataaccgt
    36841 agccccatag tataacggta agggcgagca gcacttccag caccagcacg cctatcgcca
    36901 gtgtcgaact ggagaagcta agtccttcct ctttgttgat attcaggaag ctcggaatac
    36961 cgagatacag caggtagccg gtgtaaaaca gcgccaccgt gccgaccagc gcacacaacc
    37021 agaccagcgg gtaaagcgcc accagaccgc ttaaaaacag cggagttgca acgtagcccg
    37081 cgaagaccat gcagtgcgca agtgacggac gctgcggata attacgcgcc atccaccaga
    37141 tgacccgccc catcaccgca accccagcca gcataacgcc gtaaaacaag acagccagcg
    37201 ccagtccggt aaaccaggat aacttcagga tagtgccatc gccaaaattc cagccaatct
    37261 gtgtagtgcc aatgaaggcg caaatcaccg gaatcgccgc catcagcaaa acgtggtggg
    37321 tgtagtgatg agaaatcgtt tcgttttcgc gattaatcac ctgcatttca cgatcgggat
    37381 gggaaaacag tccccagaca tggctcatac cgcccccttg ttgtgagttc atgaacctga
    37441 cagttcaagt ataagtcagc ttgtgattat tttttgttca ctcatgtaat ttgcaccgtc
    37501 tgatgtacgt cctttttcag cgtgaatgat tcacagggtg tatgcttaca ggcaagcaaa
    37561 gggagacaga ctgtcctatg gatctcaata cactcatctc acaatatggt tatgccgcgc
    37621 tggtgatcgg tagcctggcg gaaggtgaaa ccgtgacttt gctgggaggc gttgcggcac
    37681 atcaggggct attaaagttc ccgctggtgg tactttctgt ggcgcttggc ggcatgattg
    37741 gcgaccaggt gctttatctg tgcgggcggc ggtttggcgg caagctatta cgccgtttct
    37801 cgaaacatca ggataaaatt gagcgggcgc agaaactgat ccaacgccat ccgtatctgt
    37861 ttgtcattgg tacgcgtttt atgtatggct ttcgggtcat tggcccgacg ctgattggtg
    37921 ccagccagct gccgccgaaa atctttctgc cactgaatat cctcggcgca tttgcctggg
    37981 cgttgatttt taccactatc ggttacgctg gtggtcaggt gattgcgccg tggttgcata
    38041 atctcgacca gcatttgaag cactgggtct ggttgattct ggtcgtggtt ctggtggtag
    38101 gcgtgcgctg gtggctgaaa cgacgcggga agaaaaagcc ggataatcag gcgtaaaacc
    38161 attgcctagg ataaggcgtt cgcgccgcat ccgacatccg gtgcaccatg cctgatgcga
    38221 cgcttgtgcg tcttatcatg cctacccaaa tattacccat tgaaataacc acgcacctca
    38281 ctgcgctatt ctgggttgaa ctgcggattc gccagcataa agccgccatc cactatcaac
    38341 gactgcccag tggtgtaatt tgcgccctcc gaacaaagcc acaccaccag gctggcaatc
    38401 tcatgcgttg cgccaaaacg ccgcaaggga atcgaaggct ccgcgccggg cttcacgtcg
    38461 ccatcatcca tgccgttcat tggcgtggcg atcgccccag gtgcaaccgc attcaccaaa
    38521 atcttatgcc tgaccagctc cagcgccatc gctttggtta acccgccgag cgcatgttta
    38581 gcggctgtgt aggcgctggc atccggcaac ggcgtatgtt catgcaccga cgtaatgttg
    38641 atgatgcgac caccctgccc ttgtttcacc atctggcggg cagcaatttg cgagcataaa
    38701 aatgcaccat cgacatcaac ggtaaaaatc ttgcgccact catcgaaagc catatcaaga
    38761 aacggcgctt tggtcattgc gcccgcatta ttcaccagca catcaatacg ccccagccgt
    38821 tgaatgagtt tctccagcgc cagtgcccct tctggtagat tgccgagatc cagctgcacg
    38881 atctccgcac gtacgccgtg gctaactacc tcacgcgcgg tatcttttgc cccttcttca
    38941 tctgagtgcc aggtaatgcc aatatcaaac ccctgctgcg ccagtaataa cgcgcactct
    39001 ttgccgatcc ccgaatcgga ggcggtaata atcgcgacct gtgccatcga gttctccact
    39061 taacgctgaa taaacgttaa gtatagaagg cgcatatcat cagcgtttgt aacccccgcc
    39121 caacgcacca gtgagttgaa tggaggcatc cagccactgc ccttgcaata acaggccatt
    39181 ggcccgctca cgcagcgcgg ggattctggc ttcgctgacg cgggaacccg caatgatgcc
    39241 cgcgttaaag cgcgcctgcg caagacctac tacacgcaag gcatcgcgct caatttgcgc
    39301 ctgatgctgg tttttctccg ccagtgtctg aacctgactg gctgcccgcg ccacgtcatt
    39361 caccgcttca accaccgctt tgttgtagct ggcgatagac aagttgcttt cggcttttgc
    39421 gatatcgagg ttggcgttaa gacgaccact atcgaaaatg ggtagcgtca ggcctgccgt
    39481 aacgcccatt tgctgcgcgg aatgacggaa cagatcgctt aagtgcaacg catcctgttg
    39541 caggaaggcc atcaggttga tgtcaggata aaatgccgct tttgccgcat caatagtgct
    39601 taacgatgac tcaacgtacc agtgcgccgc ctgcaaatct gcccgccggg ccagtaagga
    39661 gtaccccagt tcatcaggaa gctgacttgc cactttcggc aacgcgaccg gattaagctt
    39721 caatgccttt gtctggtgat ttgtaagtgc gcttaaccgt gcctcaataa ttttcatttt
    39781 ccccgcgaca tcgttgagct gctgccgggt tttgctggca ttaatatcgg tttccacacc
    39841 ttcaactgaa gaagtaatcc cgttctgata tagctggcga tcggtggcga taatggtgtt
    39901 ctgctctttt tcgatttgct gtaagacagt gtttaacgcc gcctgggttt gccactccca
    39961 gtacaggcgg gctacgctgc cagccagcaa ttggcgggtt tgctcgcgtt ccgccgctcg
    40021 tgctttaacc gtacccaggc gagcagttac ctctgcccga ttctttcccc agatatcgag
    40081 atgccagccc gccgttaagc caaaagtacc gttggtgtac cacggaccgg tcgtacctgc
    40141 ggcaggatcg ttcagagcaa acggccccat taacccttct gcagacattt tttgccgctc
    40201 catatccgca gaaaagtcga tctgcggacc atcctgagta gcaactgcct tcgcctgggc
    40261 ttcagccagt tgaatgcgct gttcagccac ctgcatatcc ggtgcgttct gtagtgcatt
    40321 gttaattaag gaagtgagtt gattatcgtg atactccagc caccattggc tgtctggcca
    40381 accatttttc agcgccgtgg gtaatgcggt gtcaacttgt gcagcgggcg tttgctggct
    40441 taacgcctgg cgggtttcat gcataggtgc acacccggcc agcatcagta acagcggaaa
    40501 acaggcgatg gcgggataaa aggaatcacg attcatgggg gaataatcag gtaagaaaag
    40561 gtgcgcggag attaccgtgt gttgcgatat attttttagt ttcgcgtggc aatacatcag
    40621 tagcaataaa acgacatatc cagaaaaata tacactaagt gaatgatatc ttccgattta
    40681 tcttaatcgt ttatggatag cggcaaaggg cttcgttttt tcctatactt attcagcact
    40741 cacaaataaa ggaacgccaa tgaaaattat actctgggct gtattgatta ttttcctgat
    40801 tgggctactg gtggtgactg gcgtatttaa gatgatattt taaaaatcag atatggcata
    40861 taagttgtca ggtcaggaac tagatatttt gtgtacttga tttgcaaaca gctccggtgt
    40921 tggtggcgtc aaaatcgctg aagcgttccc cgaaggccgg ggcagcccac atggatgtgg
    40981 gctgagggcg cgtcttacaa ggacgttacc tcgcgcccga cccggtagcc gaaggggata
    41041 agctgaaggt accgcgcagc ggcgattttg ttcgccagag cccgggggtg cagggggagg
    41101 aggcgattgt cctccccctg cgcgctcctt gcgccagtgg caatatgtgg cttagctcat
    41161 gaaaggagcg caacaagatg atgaagctga ataagcgatt cattgtctca gataagctgc
    41221 gattccacta ttatcgcagc ttatcgatat caatggcctg aatagccctt gcaatatcag
    41281 gggaattatt caacacccga acatgctgaa ataattccgt tgcttcatcg tattctttac
    41341 gcaaataact caaccactgt ttaatccgcg caacgtgata taacccggta tcgccctgct
    41401 tttccagacg ggtatatttt tgcagcaaag caaccacctc cggccacggc attcgcggtt
    41461 cgttatattt caccacacgg ctcaggttgg gaatattgag cgccccgcga ccaatcatca
    41521 ctgcgtcgca gccgctgatc gccatgcatt gttgcgcact ctgccagtcc cagatttcac
    41581 cgttggcaat caccggaata ttaagccgct ggcgaatctc gccaatcgcc tgccagtcaa
    41641 tatgttccgc gcggtaaccc tgctcttttg tccgcccatg tactaccagc tccgtagcac
    41701 cagcctgttg aaccgcatcg gcgatttcaa atttcttctc accgctgtcc cagcccagac
    41761 gcacttttac gctgacaggc aaatgcgccg gtacagcttc gcgcatcgct tttgcaccct
    41821 ggtagatgag ttcaggatct ttcagtaacg ttgccccacc accgctaccg ttaaccgttt
    41881 tcgacgggca gccgcagtta agatccacgc cccaggaacc taactccacc gcacgggcgg
    41941 cgttctctgc cagccactgt gggaactgcc ctaacaactg cacgcgaacc agcgtaccgg
    42001 atggtgtccg gctggcattt tgtagctcag ggcaaatgcg atgaaagact tttactggca
    42061 gcagttgatc caccacgcgg acaaactcgg tgatgcacag atcgtagtcg ttaacttcgg
    42121 tcagtaattc ccgcaccaga gagtcaagta caccctccat cggagccagt aacacacgca
    42181 tatcatcacc cgcaaaaaaa tgaggcgcta tgttagcgcc tccggtcaac ggattaaagg
    42241 tctgcaaaga gggattgctg caaactgcaa tccctctcta tgcttgatgt atgcctgagt
    42301 tcgctgcgca ttgcgtaata ccggggagcg aaaaaataag cgctgatttc ttaatgtgat
    42361 cggtagcacg ttttacgaat taattgtatg atgaatccat ctcatctggg gtgttgatta
    42421 tgagtaagac actgaacatt atctggcaat atttacgtgc ttttgtcctg atttatgcct
    42481 gcctgtatgc aggcattttc attgcttccc tgctaccggt aaccattccg ggcagcatca
    42541 tcgggatgct gatcctgttt gtcctgctgg ccttgcaaat tcttccggcg aaatgggtca
    42601 atccggggtg ctacgtactg attcgctata tggcgctatt gtttgtgccg attggtgtag
    42661 gcgtcatgca atattttgat ttgctccgcg cacagtttgg tccggtagtg gtttcctgtg
    42721 cagtcagtac gctggtggtt tttctggtgg tgagctggag ttcacaactg gtgcatggtg
    42781 aacgtaaagt cgtaggtcag aaaggatcag aagaatgatg gcgaatatct ggtggtcatt
    42841 accgctgacg ttgattgtct tttttgccgc ccgcaaactg gcggcacggt ataagtttcc
    42901 cctgcttaac ccgttgctgg tagcaatggt ggtgatcatt ccttttttaa tgctgaccgg
    42961 catctcttac gacagctact ttaaaggtag cgaagtgcta aacgatctgc tgcaaccggc
    43021 ggtcgtcgca ctggcctatc ctttatatga gcagctacac cagatccgcg cgcgctggaa
    43081 atcgatcatc accatctgtt ttatcggcag cgtggttgcg atggtgacgg ggacttctgt
    43141 ggcattgttg atgggcgctt caccggaaat cgccgcgtca atcctgccga aatcagtcac
    43201 cacgcctatt gcaatggcag ttggcggcag tattggcggt attccggcaa tcagcgcagt
    43261 ttgcgtgatt ttcgtcggca tcctcggcgc tgtgtttggt catacattgc ttaatgcgat
    43321 gcgtattcgt accaaagctg cgcgcggtct ggcaatgggg acagcctcgc acgccctcgg
    43381 tacggcgcgc tgcgccgagc tggattatca ggaaggtgca tttagttcgc tggcgctggt
    43441 gttatgcggg attattactt cgctgatcgc accgttcctt ttcccgatta ttctggcagt
    43501 aatgggctaa aatttgcgat gcgtcgcgca tttttgatgt aagtttcacg cgttgcataa
    43561 ttaatgagat tcagatcaca tataaagcca tagcgggttc gtaaactgtt atcccattac
    43621 atgattatga ggcaacgcca tgcatccacg ttttcaaacc gcttttgccc aacttgcgga
    43681 taacttgcaa tctgcactgg aacctattct ggcagacaag tacttccccg ctttgttgac
    43741 cggggagcaa gtctcatcgc tgaagagcgc aacggggctg gacgaagacg cgctggcatt
    43801 cgcactactt ccgctggcgg cggcctgtgc gcgtacgcca ttgtcgaatt ttaatgttgg
    43861 cgcaattgcg cgcggtgtga gcggaacctg gtatttcggt gccaatatgg aatttattgg
    43921 tgcgacaatg cagcaaaccg ttcatgccga acaaagcgcg atcagccacg cctggctgag
    43981 tggtgaaaaa gcgcttgcag ccatcaccgt taactacacg ccttgtggtc actgccgtca
    44041 gtttatgaat gaactgaaca gcggtctgga tctgcgtatt catcttccgg gacgcgaggc
    44101 acacgcgctg cgtgactatc taccggatgc ctttgggccg aaagatctgg agattaaaac
    44161 gctgctgatg gacgagcagg atcacggcta tgcgctgact ggtgatgcgc tttctcaggc
    44221 ggcgattgcg gcggcaaacc gttcgcacat gccttacagt aagtcgccaa gcggtgtcgc
    44281 gctggaatgt aaagacggtc gtattttcag cggcagctac gctgaaaacg ccgcattcaa
    44341 cccgactctg ccgccgttgc agggggcgtt aattctgttg aatctcaagg gttatgatta
    44401 cccggatatc cagcgcgcgg ttctggcaga aaaagccgat gcgccgttga ttcagtggga
    44461 tgccacctcc gcaacgctga aagctctcgg ctgtcacaat atcgaccgcg tgcttctcgc
    44521 ttaagcctgg tgccggatgc ggcgtgaacg ccttgtccgg cttgccagcc ctttcctggt
    44581 gtcgaaaatc ccggcaaaca gtttgccgtt tcttgcgcaa aaccagcggg taaagtagcc
    44641 tgatggaaat tttccttaga tcgagtctcc tgcatgttaa agcgcgtgtt cctcagcctg
    44701 ttagtcctga tcggcttgct gctgttgact gtgctcggcc tcgatcgctg gatgagctgg
    44761 aaaaccgcgc cttatatcta cgacgaattg caggatctcc cctaccgcca ggtcggtgtg
    44821 gtgctcggca cagcaaaata ttatcggact ggcgtaatta atcagtatta tcgctaccgc
    44881 attcaaggag cgattaatgc ctataacagc ggtaaggtaa attatctatt actgagcggc
    44941 gataacgcat tgcaaagtta taatgagccg atgaccatgc gcaaagattt aatcgcagcc
    45001 ggtgtcgacc catcagatat tgttctcgat tacgcaggct ttcgtacgct ggattccatc
    45061 gtacgtacac gcaaagtttt cgatactaat gatttcatta ttatcaccca acgtttccac
    45121 tgtgagcggg cattatttat tgcgctgcat atggggattc aggctcagtg ttatgccgta
    45181 ccgtcaccga aagatatgct gtcagtacgt attcgcgaat ttgccgcccg tttcggtgcg
    45241 ctggctgacc tttatatttt taaacgtgaa ccgcgttttt tagggccgct ggtccctatt
    45301 ccggcaatgc atcaagtgcc ggaagatgcg caggggtatc ccgctgtcac acccgaacag
    45361 ttacttgaat tacaaaagaa acaaggaaag tagttatgga tgtacagcag ttttttgtcg
    45421 ttgccgtttt tttccttatc ccgatatttt gtttccgcga agcatggaaa ggctggcgcg
    45481 caggcgcgat tgataaacgg gttaaaaatg caccggaacc ggtgtatgtc tggcgagcga
    45541 aaaatcccgg actcttcttc gcttatatgg tggcatatat cggcttcgga attttatcta
    45601 tcggcatgat tgtttatctt attttctatc gttaatattt tctcttttct gttaattatt
    45661 gagaataatt attacttcac tcaataagct gcggatataa cattctgaac cgcagctatt
    45721 tgtgaatcct ttcacagttt aaatttcccc acgcgcatag ccttaatatc agcacgcaat
    45781 atttatttag cgttcgcctt aattactcat agcattaagg aagatcacat gccgcaacaa
    45841 aattatctgg atgaactcac tccggctttt acgcctttac tggcgattaa agaagcctct
    45901 cgctgtttat tatgtcatga cgctccctgt agtcaggctt gcccggcaca gaccgatccg
    45961 gggaaattta ttcgctcaat ctactttcgt aattttaaag gcgctgccga gacaattcgc
    46021 gaaaataatg ccctcggtgc cgtttgtgcc agagtgtgcc cgacggagaa attatgccaa
    46081 agcggttgta cacgtgccgg tgttgatgct cccattgata tcggtcgctt acagcgtttt
    46141 gttactgatt ttgaacaaca aactgggatg gaaatttatc agcccggtac taaaacgctc
    46201 ggcaaagtcg ccattattgg cgcaggtcct gccggattgc aggccagtgt gacgctgaca
    46261 aaccagggtt atgacgtcac gatctatgag aaagaagcac aacccggtgg ctggttgcgt
    46321 aacggcattc cgcaattccg gttaccgcaa tcggtgctgg atgcagaaat cgcccgtata
    46381 gaaaaaatgg gcgtgaccat taagtgcaat aacgaaatcg gtaaaacact caccctggag
    46441 cagctgaaag cggaaaaccg cgcggtactg gtcaccgtag ggttatcgag cggttccggg
    46501 ttatcgctgt ttgagcatag cgacgttgag attgccgttg acttcctgca acgtgcacga
    46561 caggcgcaag gcgatatcag cattcaacaa agcgcattaa ttatcggcgg cggtgatgtc
    46621 gcgatggacg tagccagcac gctgaaagtt ctcggctgtc aggcggtaac ctgcgtagcg
    46681 cgtgaagagt tagatgagtt tccggcaagc gaaaaagagt ttaccagcgc cagagagctg
    46741 ggcgtttcga tcatcgatgg atttacacca gtagccgtgg aaggcaataa agtcaccttt
    46801 aagcatgttc gtttaccggg tgaactaacg atagcggcgg acaaaattat tctcgctgta
    46861 ggtcaacatg ccgaacttgc tgctttttcc gcactggaac cgcaacgtaa caccatcgaa
    46921 acgcaacatt accagacccg cgacccgcaa gtctttgctg ctggcgatat tgttgagggt
    46981 gacaaaaccg tggtctatgc agtgaaaacc gggaaagaag ccgccggggc gattcatcac
    47041 tatttagagg gagcttgctc atgttaacga aagatctttc gattactttt tgcggcgtga
    47101 agtttcccaa cccgttctgc ctttcttctt cgccggtagg caactgctat gagatgtgcg
    47161 ccaaagccta cgacacaggt tggggcggcg tggtgtttaa aacgatcggt ttttttatcg
    47221 ccaacgaagt ctcgccgcgt tttgatcatc tggtgaaaga agataccggt tttatcggct
    47281 tcaaaaatat ggagcagatt gctgaacatc cgttggaaga gaatctggcc gccctgcgtc
    47341 ggctgaaaga agattacccg gacaaagtat tgatcgcttc aatcatgggc gaaaatgagc
    47401 agcaatggga agagctggcg cgtctggtgc aagaagctgg cgcggatatg atcgagtgta
    47461 acttctcctg tccgcaaatg acttctcatg cgatgggtag cgatgtcggg caaagcccgg
    47521 agctggtaga aaaatattgt cgggcagtaa aacgtggttc cacgctgcca atgctggcga
    47581 agatgacgcc gaatattggt gatatgtgcg aagtggcgct ggcggcgaag cgcggcggcg
    47641 cagatggcat tgcggcgatt aacaccgtta aatccatcac caatatcgat cttaatcaga
    47701 aaattggtat gccgatcgtt aacggtaaat cgagtatttc cggatattcc ggtaaagcgg
    47761 taaaaccgat cgccctacgc tttattcagc aaatgcgcac ccatccagaa ctgcgcgatt
    47821 tcccgatcag cggtatcggc ggcattgaaa cctgggaaga tgcggcggag tttttattgc
    47881 tcggttcagc gacgttgcag gtgaccaccg gcattatgca gtacgggtat cggatagtgg
    47941 aagatatggc aagcgggttg tcgcattatc tcgccgatca agggtttgat tcgttgcagg
    48001 agatggtagg tctggcgaat cacaatattg tcccggcgga agatttagac cgcagttata
    48061 ttgtctatcc ccatatcaat cttgataaat gtgttggctg tggacgctgt tatatttcct
    48121 gttacgacgg cggtcaccag gcgatggaat ggagcgagaa aacccgcaca ccgcattgta
    48181 ataccgagaa atgcgtgggt tgtctgcttt gtggtcacgt ctgcccggta ggttgtattg
    48241 atcttgggga agtgaagttt aagaaaggcg agaaagaaca cccggtaacg ttgtaaaacc
    48301 agcgacgcat ccggcattta gttcctgtcg ccagatgcgg cgtgaatgcc ttacccggca
    48361 tacaaaacaa tataaatcag tatgttgtgc tcattgtagg ccggataaaa cgcgccagcg
    48421 ttgcatccgg catttggctc ctgtcgccgg atgcggcgta aacgccttat ccggcctaca
    48481 aaaccacaca gatcatgatg ttatgtgcgt ttatgaacga atgcggacca cattcacatc
    48541 atttcttacg cgcgtatttc agtgaatcca gcgccacggc aaagataata attgcccctt
    48601 tgatgatgta ctgccagtac gggtttacgc cgatgtacgt caggccatag ttgatgacgg
    48661 taaaaataat caccccggtc accacgccaa tcaccgtccc tacaccgccg ctgaacgata
    48721 cgccgcctac cacgcacgct gcgatagcat ccagctcata catgaagccg aggttgttgg
    48781 tggcagagcc gatacgtccg gcttctaaca tcccgccgaa ggcatagaac acaccagaca
    48841 acgcgtagat catcagcagg ttcaggccga cgttgacacc agatactttc gccgcttccg
    48901 ggttaccgcc aatggcaaaa atgttcttgc cgaagcgggt tttgttccat aacacccaga
    48961 caaacaccac cgcaatcaac gcatagaagg tgatgtaaga gagacggaaa ctccccagcg
    49021 caataaagcc ttgggcaaaa gtggagaaac cactgtcaaa accggaaatt ggcgacgccc
    49081 cgacaaagtc gtaatagagc gagttaatgc cgtagacgat aatcatcgta ccgagcgtgg
    49141 taatgaacgg cgtcacgttg agataggcga taatcaagcc gttgatcaaa ccgatcaccg
    49201 caccaatggc acagacaatc agaataacca gcgcaatcgg catcgtcgcc atttccggga
    49261 acactttgtt ggcgttatcc atggactgca ataacgtcgc tgctactact gccgccagcc
    49321 ccacctggcg acctgcggaa aggtcagtcc cctgggtgac aattaaccct gccacaccaa
    49381 gcgcgataat aatacgcacc gatgactggg tgagaatatt acttaagttc aacagactta
    49441 aaaatgttgg gtcctggaaa ataataatcg ccagcaaaac taaaagaacg acgtaaatac
    49501 cgccctcttt caggtaagta agaaaacttt tcttatttaa cgcactcatg ggaagcccct
    49561 aatcttaaag gtgcaaagac gcaagacgca gaatttcgct ttgcgttgtt gtttttgtat
    49621 cgacaattcc ggaaacgaga ccattgctca tgaccagaat acggtctgtt atccctaaca
    49681 actctggcat ttcggaggag ataataataa tccccttgcc tttcttcgcc agttcggcaa
    49741 ttaactgata aatctcaaac ttagccccaa catcaatacc gcgcgtcggt tcatcgagca
    49801 ttaatatttc tggttgcgtt aacagccagc gaccaataat cactttttgc tgattaccac
    49861 cggagagcga accaatttgc gtccgatgcc ccggtgtttt tacccgcatc gaatcaatca
    49921 cccactgggt atcgcttttc atccgcgagt tatccagtaa accaacttta tttttatagt
    49981 tgcggatatt ggaaattaac gagttaaagc caatatccag ataggcataa attcccgttg
    50041 agcggcgctc ttcagttacc agtgcaaatc cgtggtttat ggcttcgttg gcattatggt
    50101 tattgatctt tttgccgtgc aacgtaatgg tgccagccga tttctcgcga ataccaaata
    50161 acgtctcaac aatatcggta cgtttcgctc ccaccagacc ggcaataccg aggatctccc
    50221 ctttatgcag atcaaacgag acatcgcgaa tcgacggctg gcgcagcgac gtcaggttac
    50281 gcacttccag aatcacttcg cccggcttgt tttctttgtc agggaaacgc tggttaagag
    50341 aacgcccaac catcatggcg atgattttgt ccatcgtcag tcctgccagc ggttcggtag
    50401 cgatccactg accgtcgcgc aatacggtaa cttcatcaca taactggaag atttcttcca
    50461 ttttatgcga gatataaaca ataccgcagc cgcgctcttt taatttacga ataatagtga
    50521 acagatgatt gacctctttt tcggttaacg aagaagtcgg ttcatccata atcacaattt
    50581 tcgcgttata ggaaaacgct ttggcgattt cgatcatctg catttgcgaa acagataatg
    50641 taccgacgcg cgcacgcgga tcgatatcaa tatccagttc atcaaaaatc gctttggttt
    50701 cgcggtacat tttgtcctga tcgacaaaca tgcctttggt gggatatcgc cccagccaca
    50761 tgttgtccat caccgaacgt tgtaatacca ggtttaactc ctgatgtacc atcgaaatac
    50821 cattttccag tgcttctttg gcggaatgga aatcgatctc tttaccctgg aataaaatgg
    50881 tgccggagtc tttttgataa ataccaaaca ggcattttaa taatgtcgat tttcctgcac
    50941 cgttttcccc cattaatgca tggatagaat gtggccggac ttttaagtta acgttatcaa
    51001 gtgccttaac accaggaaaa gacttgttga taccgctcat ttccaacaag tattcacctg
    51061 atgacggagt cgttgagctg accatataat tttaccttgt tggtcataca ataagggcgc
    51121 agtaaacgac tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg ttgtctttat
    51181 ctacgccaac ataaggtacg cggaccactt tgttgtcgat tttccagttg gtgccatcag
    51241 ccgcaccttt gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc gcctggttgt
    51301 tagcatcgtt cagtacggtg cccgccagtg caccggattt caccagcgcc agcgcttctg
    51361 gcagcgcatc gacgccaaac accggaatgc tggacttgtt gtgtgctttc agtgcttcta
    51421 ccgcgcccat tgccatcgcg tcgttgttgg cgataaccac ctcgattttg ttggcgttcg
    51481 ggccagacag ccaggcgtcc atcttatctt tcgcctgagc ggtatcccac atagcggtat
    51541 ctaactgtaa ctgttcagtt ttaatgcctt tgtcgttcaa ttctttaatc acgtaagtgg
    51601 tacgtgcttc tgcatccgga tgccccggct cacctttcag cagtacgaat tgaatctgac
    51661 cgtctttgtt cagatcccaa ccctgattcg ccgcccagtg tttagcaatc aaatcgccct
    51721 gaataatgcc ggactctttg gagtcagtgc caacgtagta ggttttgtcg tagctatcca
    51781 gcgccttacg agacggttct ttgttgaaga aaaccaccgg cacgttttgc ccacgcgctt
    51841 tctcaatcac cgtacccgca gctgccgggt caaccaggtt gattgccagt gccttcaccc
    51901 ctttcgccag caatacgtcg atctgatcgt tctgcttgga ctggtcattc tgagaatcat
    51961 tcatcagcag ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc ttgcgcacta
    52021 cagacataaa gttatcgtcg tacttataga ttgttacacc aatgcgagta tcagcagcgt
    52081 gtgcagcggc accgaataac atgctggcca tcacagcaga cagggttaac accttcttat
    52141 tcatggtatc tccggttttt cttatgcagg gtagtgcttg agataaatgc tcggcggggc
    52201 agtagagtta atgaagtgtt actgaacgcc gaagctcact ttttaaaatt cgttcttcca
    52261 tgcccggtaa cgctccagaa aacggcttta attgttgtta tgacgctatt acctcggtga
    52321 aagtgattat tcactgttac atatgggtta caacgttaaa acggtgcaat catagccatc
    52381 acattgttaa gatactgtga aatcactcac agattgaaag cggttacatc gcctgatttg
    52441 ttgagttagt gatcgccgcc gcattctggc gtgttgcgac agaatggcga cgcactaacg
    52501 tcggcataaa acagtggctg gcacgaggat caatattgtc tgctgccccc tgcaaggcca
    52561 gttcggtagc taatttagcc attgaagcaa tgggataacg cacggtcgtt aattgcgggt
    52621 cggtgtaacg ggcgatggga atatcatcga aaccgatgat tgagagatgt agcggaatcg
    52681 caatgccatt atcttttaat gctgtcagtg cgccagcagc catattgtcg ttataagcaa
    52741 atacagcggt tagttgtaga ttgcgcccca gcagttcaac catcgccgcc tcaccgcccg
    52801 gcatgtccgg cgtaccagtg ccaatccagc tttccggcgg aataatatcc tgctctttca
    52861 acgcactcat ccagcctgct ttacgcatgg cgtcatcttc aatgccgtgg ctggaagaaa
    52921 gataaccaat acgttgatga ccgttattca gcaacatgcg cgtcgccatt cgggcaccgc
    52981 tgagattatc caggcaaacg caacgatggg cgtaccccgg cacaacgcgg ttgattaaca
    53041 ccataccagg aatgttatcc ataaattgtg ccagttcatc gtcactcaat gcttttgagt
    53101 gaacaatcaa cgcattacaa cgctggcgaa ttaacacctc aatggcgtga cgctcttttt
    53161 ccgcttcatg atagctattg ccgattagca cgtatttctg atgctgctgg gcgaccagat
    53221 ccaccgcttt taccagcgcg ccgaaaaaag catcagaaac gtccatcacc accacgccaa
    53281 tggtgtcgct aacctgagtt gccagcgcct gggcattggc gtttggccga taatccagct
    53341 cactcacagc tttcattact gcttcacgcg tgtcggcact gaccagcgtg ctgttattga
    53401 gcacccggga aaccgttgcc acagagacgc cagcctgacg cgctacatca cgaatggtga
    53461 tcatattcac tacctgttat gagattgcag taaatgactg cttgctggcg gctattttgt
    53521 cagcactgaa tacaggactt cgtgaatcga gtcacagcaa tggaaacggt tacagccgtt
    53581 ttgttaacga atgtgatcca gatcgttatc ttgatgtttt agatattgtc ggtccggcag
    53641 cacgcaaagt taactgacgc cagagccatt ccaccggccc ctggcggaag taacgcagcc
    53701 agataacaga gaagaagata ttcgccagcc ataccggaat aacaaacgcc agcagctcca
    53761 gacggtcaaa ctgcataaac aaactgaggt ggtaaaaaag cgtagtacaa atcagcgttt
    53821 gcaatagata gttggtcagt gccatccgtc cgacacaggc gattgccagc accagcttaa
    53881 agcggcttaa ttgcggccag aagccataaa acagcgacgc atagccaatc gcctgaaacg
    53941 gcgcactcag ttcccgcggc atttgaagta agaaggcaca ccagcgatac gcccagtcca
    54001 gctgccattg cagggcgatg gcaggaaggt taatgatcac cccaatcgcc accagcacaa
    54061 aaccagtacg acgatagtga cgtaagctga actgcccttt cagccagccg ctgcgcatca
    54121 gcgccgcacc aatgagcatc atccctgcca gttgccagcc atactgtgcg cccagtgcca
    54181 gtaaactgtt gccaacacca tcggcacgat tattgatcgc ttcaacacca ccgtgaagct
    54241 tccagtattt ttcatacaaa atagccgatg catccggcgt ccaggcgcgg ctggtctggc
    54301 tgtcggaaat cagccccaat aacagcaaaa cgccaagccc caccagataa agcatgacgc
    54361 ctgtattaaa caggcttttt accgatggcg catcgcgcac cagccgccag cagattaagc
    54421 ccaccagtcc gtaagccagc agaatatcgc cgtcccagaa caataaaccg tgaataaagc
    54481 ccagcaagac taacagcgtt aaccgcgact ggatccagcg tctgccacgg ggcagcaaca
    54541 tttgcaggcc cgcaccaaac agcagcgcaa aaagcgtgag gaatttcacc tggccgatga
    54601 gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat agcgccgtac caggcgggat
    54661 tgagataagc cgcctttggt agcccaaagg cgctgatgtt taatagcagg atccccagaa
    54721 tggcgacgcc gcgaacaaaa tcgagcgtga cgttgcgctc catggctcct gccttttaat
    54781 cagttgtgat gacgcacagc gcgcagaaac tcgtggcgcg tattctgact ggatttgaac
    54841 aatccaccaa gagaggtcgt tgttgtggca ctggttgcgt cgcggatgcc acgcgctttc
    54901 acgcagtaat gcaccgcgtc gatagagaca gccacgttat tggtgcccag cagcgtttgt
    54961 agcgcaatga gaatttgctg cgtcagacgt tcctgcacct gcggacgctg ggcaaagaac
    55021 tgcacgatgc ggttaatttt tgacagaccg atcaccgaat ctttcgggat ataagccacc
    55081 gtcgctttgc catctatggt aacaaaatgg tgttcacagg tgctggtcag ggtgatatcg
    55141 cgcacggtga ccatttcatc gaccttcatt ttgttttcaa tgagggtgat tttcgggaaa
    55201 ttggcgtaat ccagaccgga gaaaatttca tcgacataca ttttagcgat gcgatgcggc
    55261 gtttccatca aactgtcatc agccaggtcg agattcagca gctgcatgat ttcggtcata
    55321 tgaccagcaa taaggctttt gcgcgtttcg ttatccattt catgcacggg cgggcgcagc
    55381 ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca gggccgcttc tttactgagt
    55441 gatggcattt atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg gggcaaagtt
    55501 attaagctga tttacagcct gattattgtg cgtgaggcgg cgcacataat ccagtattca
    55561 cagcgataat tattgtaatt gccgctgcct ttcatcagca gatgttaaaa catcgttatg
    55621 caaatacgga agtgaaagtt actcacagca cattgaataa acggtatgat gaagaaattg
    55681 caaacaacac aacaaggagc cacgcatgga aatgctcgaa gagcaccgct gttttgaagg
    55741 ctggcagcaa cgctggcgac acgactccag taccttaaac tgcccgatga cgttcagtat
    55801 ctttctccca ccacctcgtg atcacacccc gccaccagtg ctgtactggc tttccggatt
    55861 aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg cggcggaact
    55921 ggggattgta ctggtaatgc cagacaccag cccgcgcggc gaacaggttg ccaacgacga
    55981 tggctacgat ttaggccagg gcgcggggtt ttatcttaac gccacgcaac caccgtgggc
    56041 gacgcattac cagatgtatg attatctgcg cgatgaatta ccggcactga ttcagtcgca
    56101 atttaatgtc agcgaccgct gcgctattag cggtcattcc atgggtggtc acggcgcgct
    56161 gattatggcg ctgaaaaatc cgggtaaata caccagcgtt tccgcctttg cgccaattgt
    56221 gaatccgtgc agcgtcccgt ggggaatcaa agcgtttagc acttatttag gtgaggacaa
    56281 aaatgcatgg ctggaatggg acagttgcgc gttgatgtat gccagtaacg cgcaggatgc
    56341 aatcccgacg cttattgatc agggtgataa tgatcagttt cttgctgacc aactgcaacc
    56401 tgcagtactg gcagaagccg cgcgccagaa agcgtggccg atgacgctgc gtattcagcc
    56461 gggatatgat cacagttact acttcatcgc ctcttttata gaggatcacc tgcgcttcca
    56521 tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc tcatcagaag
    56581 cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta gtcgtcacga
    56641 ctgagatcct tgtcgccaag gttcagcacg cctgcacgca gtttgacgtc tttagtcacc
    56701 tgccaggccg cgccggtatt ccagatggta taaccgcccg gtgttttagc cgtcgcgctg
    56761 tcggcgcgtt tctgcccggt atagtgacca gaaacataga atgaccagtc ttccagcgcc
    56821 agcggtttcc agtccagcgt accgttagca gtatggaacg gcagatcgga tagcggtttg
    56881 ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt agttgatcga cagtttccat
    56941 tcatcgttga acggaatttt cagttcggtt tccacgccct gaatacgtgc tttgttaacg
    57001 ttgtagtagc taaataccgg tatgcgccgt ccgttagcgc ccgtctcaaa accgacaaag
    57061 ttttggtagc ccggtgcagc gttgacgtca gacgtacggc taatgctgat acgatctttc
    57121 acatcgttac ggaaaacggt aacgctggat tcaacccctt ccagccagcc ttcttcgccc
    57181 atgtagtaaa gccccagttc ccaactttcg ctggtttctg gtttcagatc cgggctaccc
    57241 acaatcttac atgcgccacg gcaggaattg ctcgtccagt cagggctaag ttgcaacaga
    57301 gaaggtgctt taaatgccgt cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta
    57361 taaaccaggt aggcacgcgg actccagtgt tcaccgtagg tttcgtgatc gtccatacgc
    57421 acgccggtcg tcagcgccag cggctcgaag atccgccatt catcttccac aaacagcgcg
    57481 tactggctgg cagacgtttt ggagctggtt cccccggtca ggttcaccgc atcgctaagt
    57541 ttgtcgtgac gccattcacc gccaaccgtg agaaactgat taatcgccgt cagcggcaac
    57601 gtgtatttgc cgtcgaccgt attgctttcg gaagttatcg ggctgctgtt gccagggttt
    57661 ttgttctcga ctttctcacc gtagtatttc agttcgctgg tgccgtaatc ccaacgccca
    57721 ttatggctga cggagtagtt ctggcgttcc aggcggtttt tgtccagcga gtcggaatca
    57781 cgatcctgac ggtcgaaacc gtatccggca gtaaaatcgt gattttgatt cggtgtccag
    57841 gcaaattcga cattgccgtc gcggctggag aatccttcaa tacgcggcgt ttctccggta
    57901 tcggtggtcg ttgagttttg cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct
    57961 ttcattccca gcacgccgtc aattaatggt ccgctggtaa agaactgacc gttataggta
    58021 tcaccgcgat cgcgatgttc ctgaacggtg gtatcgacgg taacggtgcc cgaccatttc
    58081 tgaccgattt ttttggtgat gatattcact acaccgccga gcgcatcgga accgtacagc
    58141 gacgacatcg ggccacggac cacttcaata cgttcgatgg aatcgaccgg gatccagttc
    58201 agatcgaaat cattgtggcg gaagacggca ttgcgggagt taacgcgttt accgtcgaca
    58261 agaatcaggg tgtagctgct gtccagacca cgaatactta cgcccttacg gttatcccct
    58321 tcgttcgtca gttgtacgcc aggcacttct ttgaggacat ccttcagatt ctgtaccggt
    58381 tttcgctgca ggtcttcctg ggtaatgacg ctgatactgg cgggagcatc tttaaggttt
    58441 tgttcaacgg aagatgcagt gacaaccatc gtttcgccat catcatcgac cgctaacaca
    58501 ggccatgcac aagaaatagc ggacaaacac agcccgaccc gtacgaaagg gttcaaccta
    58561 aacattccat atctccatga ggtaactacg aaaataaaat gggtatcgct cacatcttct
    58621 ttacgtcccc ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag gtgatcatcc
    58681 cagaaagccg gaacagcctc tgatttgtca gccttttttg attgcggcgt aacgataatg
    58741 caaacgataa caattatcaa tccaaatgtt aaattttata tccgcatgat ttgtcaggga
    58801 aatggaattg aaaaagccct ctcggttgag agggcttagc aaggaaggga ggaaacttat
    58861 ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt tcctttaatc
    58921 agcttatagc cgaaccaaat aatcaggaac agcgggatac caatatacgt tgccgctacg
    58981 ccgccccagt caatggtatc tttcaggaac gcttcgtagt tctggcccaa agtgataatc
    59041 agacacagaa tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg gtacggcaga
    59101 tcgttaatgt cgtgtccctg caatacgtac ccgcgacgga aacgatagtg gctaatggca
    59161 atccccagcc aggcgataaa acccgtcatc ccggaggtgt tcagcagcca caggtatacc
    59221 gtctggttgc caaacatgga ggtcaggaag cacaaaccgg caatcaccgt cgtcgcatac
    59281 agcgcattac gcggcacgcc accacgcgac agtttagcga aaatgcgcgg cgctttaccg
    59341 tcacacgcca gggtgtacag catacgagta gacgcataca taccggagtt acccgctgac
    59401 agcaccgccg tcagaataac tgcgttcatc accgccgccg cagagagcag acccgcgtgc
    59461 tggaacacca gggtgaacgg actgacgctg atgtctttaa catcgttacg cagcaggctc
    59521 ggatcggtgt acggaataat caggctgata atcaggatcg cgaacacata gaacaacagg
    59581 attcgccaga atacctgacg taccgcgcgt ggaatgtttt tcgccggatc ttcggactcg
    59641 cctgcagcaa taccgatcag ctcggttccc tggaaagaga agccgacaat catagctacg
    59701 ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca gttgctccag
    59761 cccgcaggct gcgcgccttt gaagataccg ataatcatca gcacgccaac gatgataaag
    59821 acaataactg tcgtgacttt gatcagtgag aaccagtatt ccgcttcacc aaagccgcga
    59881 acagagatgt agttcaacag gaagataacg ccgaggaaca acgcactcca gatccagccc
    59941 ggtgtatccg ggaaccacca gctcatgacc agctgtgctg caaccaggtc aacggcaata
    60001 gtcaccgccc agttgtacca gtagttccag cccagcgcga agccaaagcc ttcttcaaca
    60061 tagttctgac cgtacgtggc aaacgaaccg gaaaccggca tatatgcagc cagttcaccg
    60121 agactggtca tcaggaagta aaccatcagg ccaatcagca tatacgagag caatgccccg
    60181 cccggacctg cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt accgatggaa
    60241 ccgccaatgg caatcatcgt caggtgacgc gccttcaatt cacggcgtaa gcccggcgct
    60301 tctgtagttt tagtttcgga acccatacaa aaatgctatc catcttaaaa atgaggcgcg
    60361 attgtagcag acgattggca atccttccgg cagaaataca cggttataag acaccttcat
    60421 gatcgcccag ggattataag taaagcagcc aatctctttt ctggcgaatg catgctgagg
    60481 gcgctgcgtc acgaaatcga cacagcacca gcatgttctt gtacagcacc ttaacgcggt
    60541 acattcgcgg gatcgcaata gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc
    60601 cgatgatgta tacgccacaa cgtacgcatc aggcgcggca gagggaccgc aacttcactt
    60661 aatgtgcctg cctgcaattg atcttcaatc acccgtcgcg acaggcaact aattcccaac
    60721 ccatgacgca ccgcatgttt gattgcctcg gagttaccta attccatcgc catctcaaac
    60781 ttcggtaaat gtgacagcaa cagataatcg acaatctccc gcgtgccgga accgcgttca
    60841 cgcaggatcc acggcgcagc ggccagctgt tctaaggtga ccggaccacg agccaacggc
    60901 gaagtcggcg cggcgaaaac caccagctcg tcttccagcc acggttcaga aatgatttca
    60961 gtgctgtggc acggtccttc aataaagcca atatcaacgc ggaaatccag caccgcttgc
    61021 atcacgtcct ggctattccc aacgctaagt tcaatcggca actgcggata atcatggcga
    61081 taacgggcga taactgcagg tagaatgtag ttaccgatgg tactactggc atagatacga
    61141 atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc aagcagtgcc
    61201 aacgctcgcg gatagagcag ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga
    61261 tcaaacagtt gaacgccaag ctgcccttcc aggtcggtca aggctgcgct cactgctgat
    61321 tgcgacaacg ccagcatcac cgacgcctgg gtggttgatc cacttttcaa tacttctgca
    61381 aaaacttcca actgccggag ggtgatgtgc atagtcgctt accacttat
//
LOCUS       DAPQZD010000008        58766 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPQZD010000008 DAPQZD010000000
VERSION     DAPQZD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.008"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            4..2481
                     /locus_tag="RO454_000524"
     CDS             4..2481
                     /locus_tag="RO454_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0932406.1"
                     /translation="MLLPLHRTFVLTGITFALSAVYSLSYARDEFNLRILELDSPLEN
                     TQVLEDFVNNNNLTPGVYLTSVMWGQEYLDKRNITFILSSDKKRLIPRFTKADLREFG
                     LKVDDIPALQVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISP
                     KYWSDGESAVWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWE
                     SISTSLQRDIKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVR
                     GIANSDAQVVIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQ
                     ASSTVPILQREGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGAS
                     IYHSGVTGIGADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVT
                     LASYRYSTSQFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYL
                     NGYQQDYWGMHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSR
                     WLPDSWAMYNVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRY
                     RSSVGEFGLGYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKV
                     QNAQGVYTDYWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDF
                     DARSGIRALLTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKE
                     INQQCTGVVTVPEKYTTGIYKATMDCR"
     gene            2503..3513
                     /locus_tag="RO454_000525"
     CDS             2503..3513
                     /locus_tag="RO454_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0932407.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFKIQIEKCSQDVKVDYRFTSSGSTGVTGNNILNIESGSGMAEGV
                     GLQILDSNNNVMVFDTDYTPVQKTTLNQNVTIPLKARYIKMGSVKSGRVNSVATFEVY
                     YR"
     gene            3733..5982
                     /gene="fhuA"
                     /locus_tag="RO454_000526"
     CDS             3733..5982
                     /gene="fhuA"
                     /locus_tag="RO454_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDY0932408.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            6034..6831
                     /gene="fhuC"
                     /locus_tag="RO454_000527"
     CDS             6034..6831
                     /gene="fhuC"
                     /locus_tag="RO454_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDY0932409.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            6831..7721
                     /gene="fhuD"
                     /locus_tag="RO454_000528"
     CDS             6831..7721
                     /gene="fhuD"
                     /locus_tag="RO454_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDY0932410.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            7718..9700
                     /gene="fhuB"
                     /locus_tag="RO454_000529"
     CDS             7718..9700
                     /gene="fhuB"
                     /locus_tag="RO454_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDY0932411.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(9735..11015)
                     /gene="hemL"
                     /locus_tag="RO454_000530"
     CDS             complement(9735..11015)
                     /gene="hemL"
                     /locus_tag="RO454_000530"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDY0932412.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            11240..12661
                     /gene="clcA"
                     /locus_tag="RO454_000531"
     CDS             11240..12661
                     /gene="clcA"
                     /locus_tag="RO454_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDY0932413.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            12685..12750
                     /locus_tag="RO454_000532"
     CDS             12685..12750
                     /locus_tag="RO454_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932414.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            12743..13087
                     /gene="erpA"
                     /locus_tag="RO454_000533"
     CDS             12743..13087
                     /gene="erpA"
                     /locus_tag="RO454_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDY0932415.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(13134..13757)
                     /locus_tag="RO454_000534"
     CDS             complement(13134..13757)
                     /locus_tag="RO454_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDY0932416.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(13795..14595)
                     /gene="btuF"
                     /locus_tag="RO454_000535"
     CDS             complement(13795..14595)
                     /gene="btuF"
                     /locus_tag="RO454_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDY0932417.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(14588..15286)
                     /gene="mtnN"
                     /locus_tag="RO454_000536"
     CDS             complement(14588..15286)
                     /gene="mtnN"
                     /locus_tag="RO454_000536"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDY0932418.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            15370..16887
                     /gene="dgt"
                     /locus_tag="RO454_000537"
     CDS             15370..16887
                     /gene="dgt"
                     /locus_tag="RO454_000537"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDY0932419.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            17017..18441
                     /gene="degP"
                     /locus_tag="RO454_000538"
     CDS             17017..18441
                     /gene="degP"
                     /locus_tag="RO454_000538"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDY0932420.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            18596..19753
                     /gene="cdaR"
                     /locus_tag="RO454_000539"
     CDS             18596..19753
                     /gene="cdaR"
                     /locus_tag="RO454_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDY0932421.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(19807..20193)
                     /locus_tag="RO454_000540"
     CDS             complement(19807..20193)
                     /locus_tag="RO454_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDY0932422.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(20503..21327)
                     /gene="dapD"
                     /locus_tag="RO454_000541"
     CDS             complement(20503..21327)
                     /gene="dapD"
                     /locus_tag="RO454_000541"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDY0932423.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(21358..24030)
                     /gene="glnD"
                     /locus_tag="RO454_000542"
     CDS             complement(21358..24030)
                     /gene="glnD"
                     /locus_tag="RO454_000542"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDY0932424.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(24092..24886)
                     /gene="map"
                     /locus_tag="RO454_000543"
     CDS             complement(24092..24886)
                     /gene="map"
                     /locus_tag="RO454_000543"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDY0932425.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            25254..25979
                     /gene="rpsB"
                     /locus_tag="RO454_000544"
     CDS             25254..25979
                     /gene="rpsB"
                     /locus_tag="RO454_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDY0932426.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            26114..26965
                     /gene="tsf"
                     /locus_tag="RO454_000545"
     CDS             26114..26965
                     /gene="tsf"
                     /locus_tag="RO454_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDY0932427.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            27112..27837
                     /gene="pyrH"
                     /locus_tag="RO454_000546"
     CDS             27112..27837
                     /gene="pyrH"
                     /locus_tag="RO454_000546"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDY0932428.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27987..28544
                     /gene="frr"
                     /locus_tag="RO454_000547"
     CDS             27987..28544
                     /gene="frr"
                     /locus_tag="RO454_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDY0932429.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28636..29832
                     /gene="ispC"
                     /locus_tag="RO454_000548"
     CDS             28636..29832
                     /gene="ispC"
                     /locus_tag="RO454_000548"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDY0932430.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            30021..30779
                     /gene="ispU"
                     /locus_tag="RO454_000549"
                     /gene_synonym="uppS"
     CDS             30021..30779
                     /gene="ispU"
                     /locus_tag="RO454_000549"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDY0932431.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30792..31649
                     /gene="cdsA"
                     /locus_tag="RO454_000550"
     CDS             30792..31649
                     /gene="cdsA"
                     /locus_tag="RO454_000550"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDY0932432.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31661..33013
                     /gene="rseP"
                     /locus_tag="RO454_000551"
     CDS             31661..33013
                     /gene="rseP"
                     /locus_tag="RO454_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDY0932433.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            33043..35475
                     /gene="bamA"
                     /locus_tag="RO454_000552"
     CDS             33043..35475
                     /gene="bamA"
                     /locus_tag="RO454_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDY0932434.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35597..36082
                     /gene="skp"
                     /locus_tag="RO454_000553"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35597..36082
                     /gene="skp"
                     /locus_tag="RO454_000553"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDY0932435.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            36086..37111
                     /gene="lpxD"
                     /locus_tag="RO454_000554"
     CDS             36086..37111
                     /gene="lpxD"
                     /locus_tag="RO454_000554"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDY0932436.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            37216..37671
                     /gene="fabZ"
                     /locus_tag="RO454_000555"
     CDS             37216..37671
                     /gene="fabZ"
                     /locus_tag="RO454_000555"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDY0932437.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37675..38463
                     /gene="lpxA"
                     /locus_tag="RO454_000556"
     CDS             37675..38463
                     /gene="lpxA"
                     /locus_tag="RO454_000556"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDY0932438.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            38463..39611
                     /gene="lpxB"
                     /locus_tag="RO454_000557"
     CDS             38463..39611
                     /gene="lpxB"
                     /locus_tag="RO454_000557"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDY0932439.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39608..40204
                     /gene="rnhB"
                     /locus_tag="RO454_000558"
     CDS             39608..40204
                     /gene="rnhB"
                     /locus_tag="RO454_000558"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDY0932440.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            40241..43723
                     /gene="dnaE"
                     /locus_tag="RO454_000559"
     CDS             40241..43723
                     /gene="dnaE"
                     /locus_tag="RO454_000559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDY0932441.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43736..44695
                     /gene="accA"
                     /locus_tag="RO454_000560"
     CDS             43736..44695
                     /gene="accA"
                     /locus_tag="RO454_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDY0932442.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44793..46934
                     /gene="ldcC"
                     /locus_tag="RO454_000561"
     CDS             44793..46934
                     /gene="ldcC"
                     /locus_tag="RO454_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDY0932443.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46991..47380
                     /locus_tag="RO454_000562"
     CDS             46991..47380
                     /locus_tag="RO454_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDY0932444.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            47445..48758
                     /gene="tilS"
                     /locus_tag="RO454_000563"
     CDS             47445..48758
                     /gene="tilS"
                     /locus_tag="RO454_000563"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDY0932445.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(48792..49052)
                     /gene="rof"
                     /locus_tag="RO454_000564"
     CDS             complement(48792..49052)
                     /gene="rof"
                     /locus_tag="RO454_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDY0932446.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(49039..49239)
                     /locus_tag="RO454_000565"
     CDS             complement(49039..49239)
                     /locus_tag="RO454_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDY0932447.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            49405..49950
                     /locus_tag="RO454_000566"
     CDS             49405..49950
                     /locus_tag="RO454_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDY0932448.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            49947..50369
                     /gene="arfB"
                     /locus_tag="RO454_000567"
     CDS             49947..50369
                     /gene="arfB"
                     /locus_tag="RO454_000567"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDY0932449.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            50383..51093
                     /gene="nlpE"
                     /locus_tag="RO454_000568"
                     /gene_synonym="cutF"
     CDS             50383..51093
                     /gene="nlpE"
                     /locus_tag="RO454_000568"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDY0932450.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(51249..52073)
                     /locus_tag="RO454_000569"
     CDS             complement(51249..52073)
                     /locus_tag="RO454_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDY0932451.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(52126..53844)
                     /gene="proS"
                     /locus_tag="RO454_000570"
     CDS             complement(52126..53844)
                     /gene="proS"
                     /locus_tag="RO454_000570"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDY0932452.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(53955..54662)
                     /gene="tsaA"
                     /locus_tag="RO454_000571"
     CDS             complement(53955..54662)
                     /gene="tsaA"
                     /locus_tag="RO454_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDY0932453.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54659..55063)
                     /gene="rcsF"
                     /locus_tag="RO454_000572"
     CDS             complement(54659..55063)
                     /gene="rcsF"
                     /locus_tag="RO454_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDY0932454.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(55181..55996)
                     /gene="metQ"
                     /locus_tag="RO454_000573"
     CDS             complement(55181..55996)
                     /gene="metQ"
                     /locus_tag="RO454_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDY0932455.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(56036..56689)
                     /gene="metI"
                     /locus_tag="RO454_000574"
     CDS             complement(56036..56689)
                     /gene="metI"
                     /locus_tag="RO454_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDY0932456.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56682..57713)
                     /gene="metN"
                     /locus_tag="RO454_000575"
     CDS             complement(56682..57713)
                     /gene="metN"
                     /locus_tag="RO454_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDY0932457.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57901..58473
                     /gene="gmhB"
                     /locus_tag="RO454_000576"
     CDS             57901..58473
                     /gene="gmhB"
                     /locus_tag="RO454_000576"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDY0932458.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        14098 a        14267 c        15982 g        14419 t
ORIGIN      
        1 tttatgctgc taccattgca tcgcacattt gtcttaactg ggataacatt tgcattatcg
       61 gcagtgtatt cattgagcta tgcacgagat gaatttaacc ttcgtatcct ggagctggat
      121 agcccacttg aaaatacaca agtgctggag gattttgtta ataataataa cttaactccg
      181 ggagtatatc ttacctctgt tatgtggggg caagagtatc ttgataagag aaatattacc
      241 ttcattctgt cttcggataa aaaaaggctc atccctcgtt ttacaaaggc ggatctccgt
      301 gagttcggtc ttaaagttga cgacattccc gcgttacaag taatggatga tgacactgag
      361 tttggtgata ttgctcagat tattgatggc gcacgttatg atttccagtt agacagccaa
      421 acgctgtgtc tgcggatccc gcaaatctat cagaatgcgc gggccgcagg gagtatttca
      481 ccgaaatact ggagtgatgg tgagagtgcg gtttggttaa gttattacgc cagtggttcc
      541 cggcaaaaca gtgatggcga taacctgaat tcaaattggt tgaatttaaa ttcagggatt
      601 aatttaggcg tatggcggct acgaaataat accgtttaca gtgactccag ttgggagtcg
      661 atcagcacca gtttgcaacg cgatatcaaa gcattaagaa gccagatgga agtcgggcag
      721 acgttcacaa atggcgatct ttttgacagt gtgcagatga caggtataaa gcttgaaacg
      781 gacaccagta tgctgcctga cagcgaacaa ggctttgcgc cagtggttcg tggtattgcc
      841 aacagcgatg cacaagttgt gattaaacaa aacggctatg ttatttacca aacgtgggta
      901 tcggcagggc catttgaaat taaagactta agccaggtaa cggcgggggc cgatctggaa
      961 gtcaccatca aagaaaccaa cgggcaagaa cacagtttta ttcaggccag ttcaacggtt
     1021 ccaattctac agcgagaagg cgcattaaaa tatagtcttg caactggaaa ataccgtgac
     1081 aacgataatc atgctgagac acctgttttt ggtgttgcaa ccgcgatata tggcttgccc
     1141 tatggaataa cgatttatgg cggcatactt ggtgcctcaa tttatcattc tggtgtgaca
     1201 gggattggcg ctgatttagg ccgtctgggg tcggtatctg ttgatatcac cgcagcagaa
     1261 acgaagtttg atgatgggcg tgatgatgct acaggactgt catggcgtgc acagtatgcg
     1321 aaagattttc cagacactga tacgacagta actctggcaa gttatcgtta ttctacatca
     1381 caattttaca ctttccagga agcacttgat caacgtgata cacctgatga caagggtatt
     1441 tacagttatc gacagacaaa taatcggcgt aatcgtttac aaattaatct ttcacaaaat
     1501 attggtcgtt ggggctcggt ctaccttaac ggttatcaac aggattattg gggaatgcat
     1561 ggggccgaac gcagtatagg aatgggatac agtacgacct ggagtaacat taattggtct
     1621 gttaattaca cactaacgaa aacgccagga atggctggtg agcagcaatt ttcattaaca
     1681 ctcaatattc ctctctctcg ctggctgcca gattcatggg cgatgtataa cgtaaaccgt
     1741 tccgataaaa gtaatacatc acatcaattg ggaattggcg gtactgctct gcaagataac
     1801 aacctgagtt ataatttaca gcaaagttat actgataata atgtgggtta tgatgccagc
     1861 atgaatggac gttatcgtag ctcagtgggg gaattcggtc tgggatatag ctatgataaa
     1921 aatagtcgtc agtggaatta cagtgcgcaa ggtgcggtgg ttgctcatgc tcatggagta
     1981 acattggggc aatcagtgca ggattcattc gcgatcgtgc atattaatga gggtgccaat
     2041 gtaaaagtac aaaatgcgca aggcgtttat actgattatt ggggaaacgc gattgttccc
     2101 aatatgacga attatcgaca taacgctatt actgtgaata cacaggggca cgatagtctt
     2161 gatatttctg atgccacaca agatgttatt ccttcgaaag gggctgttgt tggtgtcgat
     2221 tttgatgcac gtagcggaat acgagccttg ttgacattag tccataataa agaacgcgta
     2281 ccctttgggg cattgctgac attagggaat agtacagcga ttgttggtga agatggggag
     2341 gtttatatta ctggtgtgca agaatcaatg acatttacgg tacagtgggg aaaagagatt
     2401 aaccagcaat gtacaggagt tgtcacagtg ccagaaaaat acaccacggg tatctataaa
     2461 gccacaatgg attgccgata acaaataaga caggaaaaaa taatgaagcc aggattcgtc
     2521 tcttatatac gaacattatt gaccataata tttcttagct ggggcgtcat tcctgaggtt
     2581 ttcgccggtt gtaacatttc tggaaatgga ggaaatccgg caacagtaca attctccgtg
     2641 cctgcgttgg ttgttaatag cgatgccgtt cctggaacga ttattgcgac agtaaatgcg
     2701 aacagtccgt caatttcagt ttcctgtacg gcaaatggtg agatctggca aggatatacc
     2761 gtattgacaa acggtgatat gcgtactgat aacccactta acaatgtgtt tcagactaat
     2821 gtgccaggaa tcggttttcg cgcggcgtgg gcgaataaca ccagtgcgca actcaccgcc
     2881 ggatatttaa tgacgccctg gcataaaggc tcgtcaacag tgattaaagg atatggttat
     2941 ccgattacaa tacatgcagc tattcagttt attgtcacag gacctgtcgg taatgggacc
     3001 attgatacca gcaaactggt tgctgactgg aagtatgaca atcgtgttat tggagagctt
     3061 cgttttagtt ctgttccggt caatattcaa ccgggcacat gcgaccttat agaaaaaaat
     3121 attaccgttc cactcaatga tattggcaca gatgattttg atgttaacaa tgtgtcacct
     3181 gtagtttccg atgatcggtt taaaattcag attgaaaaat gttctcagga tgtaaaagtt
     3241 gattaccgct ttacttcatc aggttctact ggagtaacgg gtaataatat tcttaatatt
     3301 gagtctggtt caggaatggc ggaaggagtt ggtctgcaga tcctcgatag taataacaat
     3361 gtgatggtat ttgatacaga ctatacacct gttcaaaaaa cgacgttaaa tcaaaatgtt
     3421 actattccgt taaaagcacg ctacatcaaa atgggtagcg tcaaaagtgg tcgggttaat
     3481 tctgttgcca ccttcgaagt gtattaccga taagcttttc cctttgccat aaatgttaat
     3541 ttttggtaat ggaattttta tggtttacgg tgcaaagatt aacatttttg attcatctga
     3601 ttgattctcg ccccttcatg gacgcacaga gcgtatatca cttgcgaacc cgccagcgtt
     3661 tcgaatatta tcgcatcttt ataataatca ttctcgttta cgttatcatt cactttcatc
     3721 agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact gcgtaaaatc
     3781 gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc agcggttgaa
     3841 ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag cgcatggggg
     3901 ccagctgcaa ctattgcggc gcgacagtcc gctaccggca ctaaaactga tacgccgatt
     3961 caaaaagtac ctcaatctat ttctgtcgtc accgctgaag agatggcact gcatcagcct
     4021 aagtcggtga aagaagcgct tagctatacc cctggcgttg ctgtgggaac ccgtggcgca
     4081 tctaatactt atgattacct gatcatccgc ggatttgcgg cagacggcca aagccagaac
     4141 aactatctga atggcctgaa gatgcagggc aacttctata acgatgcggt tattgaccca
     4201 tatatgctgg agcgcgctga aatcatgcgt ggcccggttt ccgtgctgta tgggaaaagc
     4261 agcccaggcg gcctgttgaa catggtcagc aagcgcccga ccactgaacc gttgaaagaa
     4321 gttcagttta aggccgggac tgacagccta ttccagactg gttttgactt cagcgatgcg
     4381 ctggatgatg acggcgttta ctcttatcgc ctgaccggcc ttgcgcgttc tgcgaatgcc
     4441 cagcagaagg gggctgaaga gcagcgttat gctattgctc cagcgtttac ctggcgtccg
     4501 aatgataaaa ccaatttcac tttcctttct tacttccaga acgagccgga aaccggttat
     4561 tacggctggt tgccgaaaga ggggactgtc gaaccgctgc caaacggtaa gcgtctgccg
     4621 accgacttta acgaaggcgc gaagaacaac acctattctc gtaacgagaa gatgattggt
     4681 tatagcttcg accacgaatt taacgacacc tttactgtgc gtcagaacct gcgctttgca
     4741 caaaataaag tctcgcaaaa gagcgtatat ggctacggca tgtgctcaga cccgctctat
     4801 tcttcaaacc cctcttcgag tccatgtgct aacgtgccgc agtctcaatg gggccataca
     4861 ctgactcgtc agtacgttat cgataatgag aagttagaaa acttctctgt tgatactcag
     4921 ttgcaaagca aattcgcaac cggttcagtg gatcacactc tgctgactgg cgttgacttt
     4981 atgcgtatgc gtaatgacat cgattcctgg tttggttatg ccggttcagt tgcaccatct
     5041 gatatctata atttagaccg tagtgacttt gattttggcg ctcacccgaa tccgtctggt
     5101 ccataccgcg ttctgctgaa acagaaacaa accggcctgt atgttcagga tcaggcgcag
     5161 tgggataaag tgctggttac gcttggcggt cgctatgact gggccgatca gtcatctttc
     5221 aaccgtgatt acggtaataa atctgaacgt gacgacaaag agttcacctg gcgtggtggt
     5281 gtaaactacc tgttcgacaa cggcgtaacg ccttacttca gctacagtga atcatttgaa
     5341 cccgcttcac agactgatgc gaacggcgat ctgtttgcac cttcgaaagg caaacagtat
     5401 gaagtcggcg tgaaatatgt gccagaagat cgcccaattg tggtgactgg cgcactgtat
     5461 cagcttacca aaactaataa cctgatggcg gatccgaacg gttcactgtt ctcggttgaa
     5521 ggcggtgaga ttcgtgcgcg tggcgtagag ctggaagcga aagcggcact gtcggcgagt
     5581 gttaacttgg ttggttctta cacctatact gacgcggaat acaccactga caccaactac
     5641 aaaggaaata cgcctgcaca ggtgccaaaa cacatggctt cgctgtgggc tgactatacc
     5701 ttctttgacg gtccgctttc aggtctgacg ctgggcaccg gtggtcgtta tactggctcc
     5761 agctatggtg atccggctaa ctcctttaaa gtgggaagtt atacggtcgt ggatgcgtta
     5821 gtgcgttatg atctggcgcg agtcggcatg gcgggctcca acgtggcgct gcatgtcaac
     5881 aacctgttcg atcgtgaata cgtcgccagc tgctttaaca cttatggttg cttctggggc
     5941 gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt tttggggcac
     6001 ggatttccgt gcccgtttca caagttggct gttatgcagg aatacacgaa tcattccgat
     6061 accacttttg cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct tttgcatccg
     6121 ctgtcgttaa cctttcctgc cgggaaagtg accggtctga ttggtcacaa cggttctggt
     6181 aaatccactc tgctcaaaat gcttggtcgt caccagccac cgtcggaagg ggagattctt
     6241 attgatgccc aaccgctgga aagctggagc agcaaagcgt ttgcccgcaa agtggcttat
     6301 ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt ggcgattggt
     6361 cgttacccgt ggcatggcgc gctggggcgc tttggtgttg cagatcgcga aaaggtcgag
     6421 gaagctatct cgctggttgg cttaaaaccg ctggcgcatc ggctggtcga tagcctttct
     6481 ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag ccgctgtctg
     6541 ttgctcgacg aaccgacttc ggcgctggat atcgcccacc aggttgatgt actggcgctg
     6601 gtgcatcgtt taagtcagga gcgtggcctg acggtcattg ccgtgctgca cgatatcaat
     6661 atggcggcgc gctactgtga ttatctggtc gccctacgcg gcggtgaaat gattgctcag
     6721 ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa tgatttatgg catcccgatg
     6781 ggtattctgc cgcatccggc gggtgctgca cctgtgagtt ttgtttattg atgagcggct
     6841 tacctcttat ttcgcgccgc cgactgttaa cggcgatggc gctttctccg ttgttatggc
     6901 agatgaatac cgcccacgcg gcggttattg atcccaatcg tattgtggca ctggagtggt
     6961 tgccggtgga gttactgctg gcgctcggca tcgtgcctta cggcgtggcg gacaccatca
     7021 actatcgcct gtgggtcagc gaaccgccat tgccggactc agtgattgac gttggtttgc
     7081 gtacagaacc caaccttgaa ctgctgaccg aaatgaaacc gtcgtttatg gtctggtctg
     7141 caggatatgg cccttcacca gaaatgctgg cacgtattgc gccggggcgc ggatttaact
     7201 tcagcgacgg caaacagcca ctggcgatgg cgcgtaaatc actgacggaa atggcagatt
     7261 tacttaacct gcaaagcgca gcggaaacgc atttagcgca ctatgaagac tttatccgca
     7321 gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt attgctgacg acgcttatcg
     7381 atccgcgcca tatgttggtc ttcggtccaa acagcttgtt ccaggaaatt ctcgatgagt
     7441 acggcatccc aaatgcctgg caaggggaaa ccaacttctg gggcagtacc gccgtcagta
     7501 tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg ttttgatcac gacaacagca
     7561 aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc catgccgttt gtccgcgccg
     7621 gacgctttca gcgcgtacct gcagtttggt tctatggtgc gacgctttct gcaatgcact
     7681 ttgtgcgtat tctggataac gccatcggag gtaaagcgtg agtaaacgaa ttgcgctttt
     7741 tccggtgtta ttgctggcgc tgctggtggt tgctgctgcg gcgttgacct ggatgaactt
     7801 ctcgcaggcg ctgccgcgca gccagtgggc gcaggccgcc tggtcgccgg atattgacgt
     7861 catcgagcag atgatttttc actacagctt gttgccgcgt ctggcgattt cgctgctggt
     7921 gggcgcgggc ctggggatgg tgggcgtgct gtttcagcaa gtgctgcgta acccgctggc
     7981 ggaaccgacg acgttgggtg ttgcaacagg cgcgcaactg gggattaccg tcaccacgct
     8041 ctgggcgatc cccggcgcga tggcgagcca gtttgctgcg ctggcagggg cttgtgttgt
     8101 tggcttaatc gtctttggcg tcgcgtgggg gaaacggctt tcgccggtaa cgctgatcct
     8161 cgcggggctg gtagtgagcc tttattgcgg cgcaatcaat cagttactgg ttatcttcca
     8221 tcatgaccaa ctgcaaagca tgttcctgtg gagcaccgga acgctgacgc aaaccgactg
     8281 gggcggcgtt gagcgtttat ggccgcagct gctgggcggc gtgatgctga cgttattgct
     8341 acttcgcccg ttaactctga tggggcttga tgatggcgtg gcgcgcaatc tcgggctggc
     8401 cttgtcgctc gctcgtctgg cggcgttgtc gctggcgatt gtcatcagtg cgctgctggt
     8461 gaacgcggtg gggattatcg gctttatcgg tttgttcgcg ccactgctgg cgaaaatgct
     8521 gggggcgcgg cgtttgttgc cacggttgat gctggcatcg ctgattggtg cgttaattct
     8581 gtggctttct gatcaaatca tcctctggct gactcgcgtg tggatggaag tgtctaccgg
     8641 ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg ctgctgccgc gtttacgcag
     8701 cattagcgcg ccggatatga aggtcaacga tcgtgtcgcg actgaacgcc aacacgtgct
     8761 ggcgtttgct ctcgcgggcg gcgtgctgct gttgatggct gtggtggtgg cgctgtcgtt
     8821 tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg ttgctcgagg atttgatgcc
     8881 ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggt gtcatgctgg cggtggcggg
     8941 ctgtattatt cagcggctga ccggaaaccc gatggcaagc ccggaagtgc tggggattag
     9001 ctccggcgcg gcgtttggcg tggtgttaat gctgtttctg gtgccgggta atgcctttgg
     9061 ctggctgtta cccacaggca gtctcggggc ggcggtgacg ctgttgatca ttatgatcgc
     9121 cgccggacgc ggtggatttt ccccacaccg catgttactg gcggggatgg cgttaagtac
     9181 cgcgttcacc atgcttttga tgatgttgca ggcaagtggt gacccgcgaa tggcgcaagt
     9241 tctgacctgg atttccggtt cgacctacaa cgcgaccgat gcgcaggtct ggcgtactgg
     9301 aattgtgatg gtgattttgc tggcgattac cccgctgtgc cgccgctggc tgaccatttt
     9361 accgctgggt ggtgataccg cacgagccgt ggggatggcg ctgacgccga cgcgaattgc
     9421 gctgctgctg ttagcggctt gcctgacggc gaccgcgaca atgaccattg gtccgttgag
     9481 ttttgttggt ttaatggcac cgcatattgc gcggatgatg ggctttcgac ggacgatgcc
     9541 acacatcgta atttcggcgc tggtgggagg attactgctg gtgttcgctg actggtgtgg
     9601 gcggatggta ttgtttccat tccagatccc agcggggctg ctgtcgacct ttatcggcgc
     9661 gccatatttt atctatttgt tgagaaagca gagccgttaa taggatgcct cgcccggtgg
     9721 gcgaggcaaa atcatcacaa cttcgcaaac acccgacgtg cagcatcgat ggtgttattg
     9781 atatcttcca tgctgtgcgc cacagacata aagcccgctt caaacgctga cggtgccaga
     9841 taaacacctt cgtccagcat catatggaag aaacgtttaa agcgttccac gtcacaggac
     9901 atcacatcct ggtagcatgt cacggactcg gcgtcggtaa agaaaatacc gaacatgccg
     9961 ccaacgtggt ttaccaccag cggaattccg gcttcttctg ccgcttcccg cagaccttct
    10021 gccagacgtg ttgtcagctc atccagcgtc tcgtgaacgc ccggctgtgc gacttcattc
    10081 agacaggcga aaccagcagc catcgcaatc gggttaccgg aaagcgtacc cgcctgatag
    10141 accggaccgg tcggggccag cgcatccatt acatcacgac gaccaccgaa tgcgcctacc
    10201 ggcattccac cgccgatgat tttgccgagg caggtgagat ccggttccac accgtaataa
    10261 tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca cttcatcgat gatcagcaac
    10321 gcgccaaatt cgtcgcacag cgcgcgcaga cctggcagga actctggcag cggtggcacg
    10381 cagttcatat tgcctgccac cggctcgacg ataatacagg caatctcttg cgggtattgc
    10441 tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac aggttaaggt atgtttggcg
    10501 aaatctgccg gaacacccgg cgaatttggc tggcctaacg tgagtgcgcc agaaccggct
    10561 ttcaccagca agcagtcagc gtgaccgtgg taacaacctt caaatttaat aattttgtcg
    10621 cgaccggtaa aaccacgggc caggcggatg gcgctcatcg tcgcctcggt gccggagttc
    10681 accatgcgca ccatatccat ggtcgggacc agttcggtca ccagttgcgc cattttcact
    10741 tccatttcgg ttggtgcacc aaagcttaaa ccacgctcgg cggcttcaat cacggcattg
    10801 cggatcgccg gatggttatg gcccagcacc atcggccccc aggaaccgac ataatcgata
    10861 taggctttgc catcaacatc gtacagataa gcgccgtccg ctttttcgat aaacagtgga
    10921 gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca cgccgccagg gatcagctcg
    10981 cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg gttcctgatt cgtagaaaaa
    11041 gtgaatggct gctattctat gttattcata acaagttaaa tactcgtcaa acatcaggct
    11101 gcttgtactg gtgcaatcct gatttcttag agtataaaag ttttgtgcat ttgaaacatt
    11161 acgctttgca aaggattttc atggaacgtg cgagtaaaat gccgtcatct tatttgtatg
    11221 accaataagt gatcattgga tgaaaactga tactccctct ttagaaacac cgcaggccgc
    11281 gcgcctgcga cgcagacaac tgattcgcca acttcttgag cgcgataaaa ccccgttagc
    11341 cattttgttt atggcggcag tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga
    11401 caaaggtgtc gcctggttgc agaaccaacg tatgggggca ctggtacata ctgctgataa
    11461 ttatccgctt ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga tgttcggcta
    11521 ctttttggtg cgcaaatacg cgccggaagc aggtggttcg gggatcccgg aaattgaagg
    11581 ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgtg ttgccggtga aattctttgg
    11641 tgggctgggg acactcggcg gaggcatggt gttggggcgc gaagggccaa ccgtgcagat
    11701 aggcggcaat atcgggcgga tggtactcga tgttttccgc ctgaaaggtg acgaagctcg
    11761 tcatacgcta ctggcaaccg gtgctgctgc ggggctggct gcggccttta acgcgccgct
    11821 ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag tttcgctata cgttaatttc
    11881 gattaaagcg gtatttattg gcgtcattat gtcgaccatt atgtaccgga tttttaatca
    11941 tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg ccgcttaata cgctgtggct
    12001 ttatctgatc ctcggtatta tttttggcat tttcggccct atttttaata aatgggtgct
    12061 ggggatgcag gatttgctgc accgtgtgca cggtggcaat attaccaaat gggtactgat
    12121 gggcggtgcg attggcggtt tgtgtggatt gctggggttt gtggcaccag caacttcggg
    12181 cggcggtttt aacctgattc ctatcgctac cgcggggaat ttcagcatgg gaatgctggt
    12241 gtttatcttc gtcgcgcggg tcattaccac cttactctgc ttctcttccg gcgcgccggg
    12301 cggtattttt gccccgatgc tggcgctggg tactgtgctg ggaaccgctt tcggaatggt
    12361 tgccgttgag ttgtttccgc aatatcacct tgaggcgggg acgtttgcta ttgccggaat
    12421 gggggcgtta ctggcggcat ctattcgcgc gccgttaacg gggatcattc tggttctgga
    12481 aatgaccgat aactaccagc tcattttgcc aatgattatt accggtcttg gcgcaacact
    12541 attagcacaa tttaccggtg ggaaaccgct atactcggcg attcttgcgc gcacgctggc
    12601 aaaacaggaa gctgagcaac tggcgcgaag caaggccgca tcagccagcg agaatacttg
    12661 aacgaaatac cagggtatta gataatggcg attattattg ggttagaatt tgcccaattg
    12721 ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca ctgccgctgg agtttaccga
    12781 cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa gataacccga atctgaaatt
    12841 acgcgtgtat atcaccggtg gcggttgcag cggcttccag tatggtttca cctttgatga
    12901 tcaggtgaac gaaggcgata tgaccatcga aaaacagggc gttggcctgg tggttgatcc
    12961 gatgagcctg caatatctgg tcggcggttc cgttgactat accgaaggtc tggaaggttc
    13021 tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag
    13081 tatctaatct gctgttcatg tgccggataa actatccggc acatctcctc tcttcaacga
    13141 ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc cgccagccga
    13201 atcaatagcg tcacgaccat gcccatcata ctggctgttt ccagtggtac ggaaaatgtg
    13261 taataagccg tagcgtggac aataccgccg ataatacagg cagttgcgta gatttctgta
    13321 cgtaaaatca tggggatttc gcgggccagt acatcacgaa tgattccgcc gccaacgcca
    13381 gtaatgacgc ccatacaaac cgcgattaac ggaccggctt ccgcattaaa ggctttattc
    13441 acgccaatgc cgacaaacac cgccagacca acggcgtcca gcaccggcaa catccatttt
    13501 ggtaagcgtc ttggctggcg caccagcacg atggtcagca tgctggtgac cattgcaacg
    13561 accagatcgg tgggatcttt cacccaaaat accgggccgt gatccagcgc catgtcgcga
    13621 attgttccgc cgcctactgc ggtaaccacg cccaatacca gaacaccaaa agggtccata
    13681 cgcaattttc cggctaacaa aacgccagag atagcaaata ccgctgtgcc gactatatcc
    13741 agccaataga cgagcattgt tcaatcccca ctgagcacct gaaaaggtca ggcactaatc
    13801 tacctgtgaa agcgcattac agagctgttg tgcagcgagg ataatacgtg ggcttgcacg
    13861 ttcaaaccag tcactcgtga gaggaataac gggaattttg agctgttcac cccagtattg
    13921 tttgatttta ggaatttggt ccggtccgcc tgtaatgaca atcgcctgtg gcgagcgtgc
    13981 taacacctgt tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa agatgttttc
    14041 tccgccacaa acttcgagta cctggttctg aatcgactct tttccactgg taaatggcgg
    14101 attaatgccg aattgcagaa aaacacgttt tttaggttta tcagcatatt gcgctttcaa
    14161 ttgcgcgtac tgatccagca gggattgcgc ggcttgttcg gccttatccg gttgcggact
    14221 ccagggggcc agttgacgta acgcattggc aatttgttca atgcttgtcg catcgaccca
    14281 catcactttt attcccagcg aagccagctg gtcaacctgc cgctcggcat tacctccacg
    14341 ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt tccagattca tcccctgcca
    14401 ggtggaaacc tgctcaatct tttgcgcttg tggaggatag tcggaatagc tgctgacccc
    14461 aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac
    14521 gcgcggcgcg gcattgagcc acagcggcgc gagaaaagac agggcgacca gcgccctgaa
    14581 cagtgactta gccatgtgcc agtttctgca ccagtgactc aaccatcagg ctggactgtt
    14641 tagcggcaac agccaggaac tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg
    14701 agatggcgcg tactacgaca aacgggacgt tgaaattgtg gcagacatgg gcgattgccg
    14761 tcgcttccat ctctacagca atggcctgtg ggaagttgtg gcggattttc gccagaccaa
    14821 cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg ttaagattca
    14881 gttcggcaat gcaggcctca gcggcagcga tcagtttatc gtcagctttg aagcctgccg
    14941 ggcagcctgg taactgaccg tattcataac caaatgccgt gacatccgcg tcgtgataac
    15001 gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt tggtgccagg ccaccggcag
    15061 aaccggtgtt aataatcaca tctggcttgc agtgttccaa cagcaaagtg gcacccagcg
    15121 ccgcagcgac tttaccgatg cccgatttca gaagcgcaac ctcggttcca ttcagttggc
    15181 cggtatagat ttcgcaaccg cccagactga tagtttgacg gttttcgatt ttgtcacgca
    15241 gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat tttcatagat ttactcgcga
    15301 taagcccgat ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg
    15361 ggagaggaca tggcacagat tgatttccga aaaaaaataa actggcatcg tcgttaccgt
    15421 tcaccgcagg gcgttaaaac cgaacatgag attctgcgga tcttcgagag cgatcgcggg
    15481 cgtatcatca actctccggc aattcgtcgt ctgcaacaaa agacccaggt ttttccactg
    15541 gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga tggaagtcca gcaggtgggg
    15601 cgttatatcg ctaaagagat tttaagccgc ctgaaagagc ttaaattact ggagacgtat
    15661 ggtctcgatg aactgaccgg gccctttgaa agcattgttg agatgtcatg cctgatgcac
    15721 gatatcggca atccgccgtt tggtcatttt ggcgaagcgg cgataaatga ctggtttcgc
    15781 cagcgtttgt acccggaaga tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg
    15841 gcggcactac gtttacggga cggggaagaa ccgcttaacg cgctgcggcg caagattcgt
    15901 caggacttat gtcattttga aggtaacgca cagggtattc gtttggtgca tacattgatg
    15961 cggatgaatc tcacctgggc gcaggttggc ggtattttaa aatatacccg tccggcgtgg
    16021 tggcgtggtg aaacgcctga gacacatcac tatttaatga aaaagccggg ctattatctt
    16081 tccgaagaag cctatattgc ccggttgcgt aaagaactta atttggcgct ttacagtcgt
    16141 tttccgttaa cgtggattat ggaagctgcc gacgacatct cctattgtgt ggcagacctt
    16201 gaagatgcgg tagagaaaag gatatttacc gttgagcagc tttatcatca tttgcacgag
    16261 gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg tggttgaaaa tgcctgggaa
    16321 aaatcacgct caaatagttt aagccgcagt acggaagatc agttttttat gtatttacgg
    16381 gtaaacaccc taaataaact ggtaccctac gcggcacaac gatttattga taatctgcct
    16441 gcgattttcg ccggaacgtt taatcatgca ttattggaag atgccagcga atgcagcgat
    16501 cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc agatgtcgag
    16561 cagcttgaat tgcagggcta tcgggtcatt agcggattat tagagattta tcgtccttta
    16621 ttaaacctgc cgttatcaga ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc
    16681 cctattgaaa cgcgcttatt ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag
    16741 gctgtcagta aattaccgtc agattctcct gagtttccgc tatgggaata ttattaccgt
    16801 tgccgcctgc tgcaggatta tatcagcggt atgaccgacc tctatgcgtg ggatgaatac
    16861 cgacgtctga tggccgtaga acaataacca ggcttttgta aagacgaaca ataaattttt
    16921 accttttgca gaaactttag ttcggaactt cgcgctataa aacgaatctg aagaacacag
    16981 caattttgcg ttatctgtta atcgagactg aaatacatga aaaaaaccac attagcactg
    17041 agtgcactgg ctctgagttt aggtttggcg ttatctccgc tctctgcaac ggcggctgag
    17101 acttcttcag caacgacagc ccagcagatg ccaagccttg caccgatgct cgaaaaggtg
    17161 atgccttcag tggtcagcat taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg
    17221 ccgcgtaatt tccagcagtt cttcggtgat gattctccgt tctgccagga gggttctccg
    17281 ttccagagct ctccgttctg ccaggggggc ccgggcggta atggcggcgg ccagcaacag
    17341 aaattcatgg cgctgggttc cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc
    17401 aacaaccacg ttgttgataa cgcgacggtg attaaagtcc agcttagcga tggtcgtaag
    17461 ttcgacgcga agatggttgg caaagatccg cgctctgata tcgcgttgat ccagatccag
    17521 aacccgaaaa acctgaccgc aattaagatg gcggattctg atgcgctgcg cgtgggtgat
    17581 tacaccgtag caattggtaa cccgttcggc ctgggcgaga cagtaacttc cgggattgtc
    17641 tctgcgctgg gacgtagcgg cctgaacgct gaaaactacg aaaacttcat ccagaccgat
    17701 gcagcgatca accggggtaa ctcaggtggt gcgttggtta acctgaacgg cgaactgatt
    17761 ggtatcaaca ccgcgatcct cgcaccggac ggcggaaaca tcggtatcgg ttttgctatc
    17821 ccgagcaaca tggtgaaaaa cctgacctcg cagatggtgg aatacggcca ggtgaaacgt
    17881 ggtgagttgg gtattatggg cactgagctg aactccgatc tggcgaaagc gatgaaagtt
    17941 gacgctcagc gcggtgcttt cgtaagccag gttctgccga attcttccgc cgcgaaagcg
    18001 ggcattaaag cgggtgatgt gatcacctca ctgaacggta agccaatcag cagctttgcc
    18061 gcactgcgtg ctcaggtggg cactatgccg gtaggtagca aactgaccct gggcttactg
    18121 cgcgacggga agcaggttaa cgtgaacctg gaacttcagc agagcagcca gaatcaggtt
    18181 gattccagca ccatcttcaa cggcattgaa ggcgctgaga tgagtaacaa aggcaaagat
    18241 cagggcgtgg tggtgaacaa cgtgaaaacg gggactccgg ctgcgcagat cggcctgaag
    18301 aaaggtgatg tgattattgg cgcgaaccag caggcagtga aaaacatcgc tgaactgcgt
    18361 aaagttctcg acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg cgacagcact
    18421 atctacctgt taatgcagta atctccctca accccttcct gaaaacggga aggggttctc
    18481 cttacaatct gtgaacttca ccacaactcc atacatcttc atcatccttt gggcatttgc
    18541 acaatgccgt acgttacgta cttccttatg ctaagccgtg cataatggag gacttatggc
    18601 tggttggcat cttgatacca aaatggcgca ggatattgtg gcacgtacca tgcgcatcat
    18661 cgataccaat atcaacgtaa tggatgcccg cgggcgaatt atcggcagcg gcgatcgtga
    18721 gcgtattggt gaattgcacg aaggagcatt gctggtactt tcacaaggac gagtcgtcga
    18781 tatcgatgac gcggtggcac gtcatctgca cggtgtgcgg caggggatta atctaccgtt
    18841 acggctggaa ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg
    18901 taaatatggc gaactggtct gcatgacggc tgaaatgatg ctggaacagt cgcggttgat
    18961 gcacctgttg gcgcaggata gccgtttgcg ggaagaactg gtgatgaacc tgattcaggc
    19021 tgaggagaat actcccgcac ttactgaatg ggcgcaacgg ctggggatcg atctcaatca
    19081 accgcgagtg gtggctattg ttgaggtcga cagcggtcag cttggtgtgg acagcgcaat
    19141 ggcggagtta cagcaactgc aaaatgcgct gactacgccc gagcgtaata atctagtggc
    19201 gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg
    19261 ggacgcagaa gatcatcgta agcgagttga acaactgatt acccgcatga aagagtatgg
    19321 ccagctgcgt tttcgcgttt cgctgggcaa ctattttacc ggtcctggca gtattgcccg
    19381 atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg
    19441 ctgctatttt tatcaggatc tgatgttacc tgtgttactc gacagtttgc gtggcgactg
    19501 gcaggccaac gaactggcgc gaccgctggc gcggctgaaa gcgatggaca ataacggctt
    19561 gctgcggcga actctggcgg cgtggtttcg ccacaatgtg caaccgctgg caacgtcaaa
    19621 ggcgttgttt attcatcgta atactctgga atatcggctt aatcgtatat cggaactgac
    19681 cgggcttgat ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt tacaactgga
    19741 tgaagagcgg taggtcgatt acaaaattac agccgggtga gactttaccc ggcccacaga
    19801 atgacattat ttacgcgtca gtttttccag atcggcttca atctcgctga tcttattggt
    19861 tactactgac tccaggtgac gtaagtcatc gaggatctta cgcttaagat caacttcgct
    19921 gcggtcacgc tggcagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    19981 ttcctggact tctttataac cctgccccac accatcagcg acaaccgttt tacgctgacg
    20041 cggatattta aacttcacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    20101 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    20161 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    20221 ttgtgcttat tttagcattt ggcctggccc gcccggctcg actgtgatcg cagaaagcct
    20281 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtgatg
    20341 atattccatg ggatggatta gcacatttat cttcattttg cgaattacat gacagtccga
    20401 aacaagatga aaacgtattt aaaaaagggg gaaaatgagt aggacgaatt attctgagcc
    20461 agaaagcaat cgggcagcgt cgtgctgccc gtgtacatac ttttagtcga tggtacgcag
    20521 cagttcgtta ataccaactt taccgcgagt tttcgcgtca actttcttaa cgataactgc
    20581 acagtagaga ctgtatttgc catctttcga cggcagatta cctgaaacaa ccacagaccc
    20641 tgccggaacg cgaccgtagt ggatttcgcc ggtttcacgg tcataaatac gggtgctctg
    20701 accaatgtat acgcccatag agatgacgga accttcttcg acaatcaccc cttcaaccac
    20761 ttcagagcgt gcgccgatga agcagttatc ttcaataatg gtcgggttag cctgcagcgg
    20821 ctccagcacg ccgccgatac ccacgccacc ggaaaggtgg acgtttttac caatctgcgc
    20881 acaggaaccg acggtcgccc aggtatcaac catggtgcct tcatcaacat atgcgccgat
    20941 gttgacgtaa gacggcatca gcacggtgtt acgggcaatg aacgcgccct gacgcactgc
    21001 cgctggtggc acgacgcgga agccttcttt ctggaaacgt gcttcatcgt agttagcgaa
    21061 tttcatcggc actttgtcga agtagcggct ttctgcccct tcgatcacct gattatcatt
    21121 aatacggaaa gagagcagca ccgctttttt caaccactga tgcgtcaccc actgaccgtc
    21181 aattttttcc gctacacgca gtgcgccgga atccagcagg gcgatcacct gatttaccgc
    21241 ttcgcgggta acggtgtctg cattggctgg cgtgatctcg gcacggcgtt caaaagcggt
    21301 ttcaataatg ttctgtaact gctgcattgt taaactcttt tcatatcagt aaacacatca
    21361 ccctttatcg tttggattga gggcctctgt caaccgctga tgcacttcct gctgcaactc
    21421 gttattaagc gcacgccggt cagcggtggc aattatgaat aaatcttcta ctcgctcgcc
    21481 aatggttgta attcgggcgc catgaagcga aattcccaga tcggcaaaaa ttttcccgac
    21541 tcgcgccagc agtccaggtt ggtcgagggc gatcagttcg aggaacgatt tgcggtcggt
    21601 atgggtcggc aaaaacgtca cttcggtttc aacagtaaaa tggcgcaatt tagcgggttg
    21661 gcgacggggc tgcggtggct gccagctact ttgtgtcagc acctgctcca gaccaaaccg
    21721 aataacctca tgacgatctg cggatagcgg gctaccatcg ggttccagca ctataaaggt
    21781 atccatcgcc ataccgtcgc gagtggtgaa aatttgtgcg tcgtgaacac ttaaattgcg
    21841 gcggtctaat tcggcacaga cggcggcaaa cagatagggg cggtccgggc tccagataaa
    21901 aatctcggtg cctccacgcg tagcctgcgg gctaagcagt accagcggtt tgcttaaatc
    21961 atgctgtaat aaatggcgtg cgtgccaggc cagttgattt gggctatggc ggacaaaata
    22021 gttagcgcga cagcgtgacc aaatttggtg cagcgcctct tcgtcgatgt tatccatgcg
    22081 cagtagtgcc agcgcctgga gttggtgatg gcgaacccgt tcgcgcatat ccggcgtgtt
    22141 ttgcattccg cgtcgtagct gcttttcggt ggcaaaatag agttcgcgca acagactttg
    22201 cttccagcta ttccacagtg tttcgttggt ggcgcaaatg tcagccactg tcaggcatac
    22261 cagatagcgc agacgatttt ccgtttggac ttcttcggca aactgcttga tgacttccgg
    22321 gtcctgaata tcgcggcgct gagctgtcac cgacatcagc aagtgctggc gaaccagcca
    22381 ggcgaccagc tgtgtttcgc gtgagttcag cccgtggagt tcggcaaaat gcactacatc
    22441 ctgagcaccg agaatggagt ggtcgccgcc gcgtcctttg gcgatatcgt gaaacagcgc
    22501 ggcgatgaaa atcagctcag ttgacggcag acgcggccag acgtccacac acaacggatg
    22561 gcgctggcgc gtttcttcac tggcaaaact ctccagtttc aacatcacgc ggatagtatg
    22621 ttcatccacc gtgtaggcgt ggaacagatc aaactgcatc tgcccgacga tatgcgacca
    22681 ttgcggcata tacgcgccga gtacgctatg gcgatgcatt ggcaatagcc cgcgccgcac
    22741 cgctccgggg tgacgcagaa tgctcaaaaa cagttttcgc gcttccggaa tattacacag
    22801 aggttgttgc agatggcgac gggcgtggcg taactggcgt agcgtggtgg agtaaatgcc
    22861 ggtgatcgca ctgttgcgca ccatggtgta gaacatacgc aagatggctt ccggctggcg
    22921 cataaatagt gtttcatcac gcaggtcgat cagcgttccg cgtagctgaa actcatcgtc
    22981 gattggacgt ggtttttcgt cggcgggaag ggcgaggatg gcttcatcga acagttgcag
    23041 cagcatctgg ttgagttcac tgacgcggcg tgtaacgcgg aagtaatcct tcatcatccg
    23101 ctcgaccggt tcgttacctt caccactgta attcaaacgc tgggcgacgc taagctggcg
    23161 atcgaataac aggcgattat cgtaacgact gacgaccaga tgcagggcaa agcgaatgcg
    23221 ccacaatata tgcaggcatt cgtttaattc cgctcgctcc gcagaggtta agaagccaaa
    23281 ccctaccatt tcatccagcg atgttgcgcc aaaatgacgg cgggccaccc attgcagagt
    23341 gtggatatcg cgcaagccgc cggggctact tttgatgtct ggttcaaggt tgtagctggt
    23401 gccgtggtaa cgctgatggc gctggttctg ttcttcaact ttcgccgcgt agaacttgtc
    23461 ggaaggccag aatccttcgc taaaaatatg tttttgcagt tcgaggaaca gcgcaacatc
    23521 gccaattaat aagcgggatt cgattaaatt ggtggcgacg gttaaatccg ataacccttc
    23581 cagcatgcac tcttcaagcg tgcgcacgct atgacccact tccagcttta catcccagag
    23641 cagcgttaac agctcgccca ctttttgcgc ctgatcgtcc gggagctttt tacggcttaa
    23701 aatcagtaaa tcgatgtcag aaagtggatg cagctcgcca cgaccgtagc caccgacggc
    23761 gaccaatgcc aggtcggcaa tctggctgaa tcccgcttca atccataatc gttgaagaag
    23821 ctggtcgata aactcggtac gcgcctcaat caactgttct gcggagatac cattgtcaaa
    23881 ggcatcaccc agccaacgct ggaaagtatc gatatgggct tttatcccac cgacggttaa
    23941 ttcatcacgg ggccaggcgc atggattttg cggttgaccg tgcagggtgg ggagagcggt
    24001 gtttgcgtac tgttctggaa gggtattcat tgtgcgccac ccataagatt aaattattgc
    24061 attaaaaaag ccggcattcg ccggcttcat tttattcgtc gtgcgagatt atcgccggga
    24121 tggtgtcatc cttgcgtagc gtcagaattt cgcagccgtt atcagtcacc acaatagtat
    24181 gctcatattg tgcagacaag ctgcgatctt tggtttttac cgtccagcca tctttcatgg
    24241 tgcggatctc ttttttaccc gcgttgacca ttggctcgat ggtgaatgtc atccccggtt
    24301 tcagcacgac gttggtttca cgggagtcat agtgcagcac ctgcggttct tcgtggaagc
    24361 cgcgaccaat accgtgtccg caatattcac gaacgacgga gaagccttct gcttcgacaa
    24421 atttctgaat tgccgcaccg atttcgcgca gattaatgcc tggttttacc atgcgtagcg
    24481 ccaggtacag gctttcttgc gtgatgcggc acagacgttc gcccatgatg gtcggcttac
    24541 cgacgataaa cattttcgag gtatcgccgt ggaaaccatc tttgattacg gtgacatcaa
    24601 tgttaacgat atcgccatct ttcagcagct tggcgtcgtc cgggataccg tggcacacca
    24661 cttcattaat agagatgcaa acggatttcg gatagccgtg atagccgagg caggcagaaa
    24721 ccgcgtgttg ttcattaaca atgtaatcat tacagatgcg atccagctcg ccggtgctga
    24781 cgcccggttt aacatacggt tcgatcatct ccagcacttc ggcagccagt cggccagcga
    24841 cgcgcatttt ttcgatatct tctggggtct tgattgagat agccattaat tctgtccatc
    24901 agcgtcggtg ataccgacaa tatatatgta agtgccgtca atggtatcac acccgggcaa
    24961 attgagaatc attctgaatt tcgccaaacg tgccactgaa ggttttctat aatagaaaat
    25021 tcaacgtctg atgctgtaca cagcgccaac aattattggt gtccacgacg tatttgtggt
    25081 ataaagcgcg ccggacttcc gatccatttc gtatacacag actggacgga agcgacaatc
    25141 tcactttgtg taacaacaca cacgtatcgg cacatattcc ggggtgccct ttggggtcgg
    25201 taatatggga tacgtggagg cataacccca acttttatat agaggtttta atcatggcaa
    25261 ctgtttccat gcgcgacatg ctcaaggctg gtgttcactt cggtcaccag acccgttact
    25321 ggaacccgaa aatgaagccg ttcatcttcg gtgcgcgtaa caaagttcac atcatcaacc
    25381 ttgagaaaac tgtaccgatg ttcaacgaag ctctggctga actgaacaag attgcttctc
    25441 gcaaaggtaa aatccttttc gttggtacta aacgcgctgc aagcgaagcg gtgaaagacg
    25501 ctgctctgag ctgcgaccag ttcttcgtga accatcgctg gctgggcggt atgctgacta
    25561 actggaaaac cgttcgtcag tccatcaaac gtctgaaaga cctggaaact cagtctcagg
    25621 acggtacttt cgacaagctg accaagaaag aagcgctgat gcgcactcgt gagctggaga
    25681 aactggaaaa cagcctgggc ggtatcaaag acatgggcgg tctgccggac gctctgtttg
    25741 taatcgatgc tgaccacgaa cacattgcta tcaaagaagc aaacaacctg ggtattccag
    25801 tatttgctat cgttgatacc aactctgatc cggacggtgt tgacttcgtt atcccgggta
    25861 acgacgacgc aatccgtgct gtgaccctgt acctgggcgc tgttgctgca accgtacgtg
    25921 aaggccgttc tcaggatctg gcttcccagg cggaagaaag cttcgtagaa gctgagtaat
    25981 aaggcacgct cgtacgagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    26041 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    26101 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    26161 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    26221 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    26281 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    26341 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    26401 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    26461 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    26521 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gtgttctggt
    26581 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    26641 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    26701 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaattgcag agaaaatggt
    26761 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    26821 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    26881 tatccgcttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    26941 tgctgcgatg tccaagcagt cttaattatc gaaaaggagc cgcctgaggg cggcttcttt
    27001 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    27061 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    27121 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    27181 actgaaggct tcggtattga tgcaagcata ctggatcgca tggctcagga aatcaaagaa
    27241 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    27301 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    27361 accgtaatga acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    27421 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    27481 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    27541 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    27601 gcaaccaaag ttgacggcgt gtttactgct gatccggcga aagatccaac cgcaaccatg
    27661 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    27721 gccttcacgc ttgctcgtga ccataaatta ccgattcgtg ttttcaacat gaacaaaccg
    27781 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    27841 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggatc ttacccgtaa
    27901 tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc tggtctgact
    27961 gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat gctgaagtac
    28021 gcatggataa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg
    28081 cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg acgccgctgc
    28141 gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac gtgtttgatc
    28201 gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga
    28261 actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag
    28321 atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc
    28381 gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg
    28441 acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg
    28501 aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa
    28561 aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt attctgtctc
    28621 aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc
    28681 agcacgctgg acgtggtgcg ccataatccc gaacatttcc gcgtagttgc gctggtggcc
    28741 ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgccgta
    28801 atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca ggggagccgc
    28861 accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttaa ggatgttgat
    28921 caggtgatgg cagccattgt tggcgctgct ggactgttac ctacgcttgc tgcgatccgc
    28981 gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt
    29041 atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc
    29101 atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa
    29161 aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg
    29221 cgcgatttgg caacaatgac gcccgatcaa gcctgccgtc atccgaactg gtcgatggga
    29281 cgtaaaattt ccgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg
    29341 cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg
    29401 attcactcaa tggtgcgcta ccaggacggc agtgttctgg cgcagctggg tgaaccggat
    29461 atgcgtacgc caattgctca caccatggca tggccgaatc gcgtgaactc tggcgtgaag
    29521 ccgcttgatt tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat
    29581 ccatgtctga aactggcgat ggaggcgttt gaacaaggcc aggcagcgac gacagcattg
    29641 aatgccgcaa atgaaatcac cgtggctgct tttcttgcgc aacaaatccg ctttacggat
    29701 atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg
    29761 gatgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt
    29821 ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc gtagggcttc
    29881 agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc aggtatgccg
    29941 tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg taaaccctgt
    30001 cagggaataa gaaacgcgtg atgttgtctg ctactcaacc acttagcgaa aaattgccag
    30061 cgcatggctg tcgtcatgtt gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc
    30121 aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc cgggctgtct
    30181 cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt agtgaaaact
    30241 ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg gcgctagata
    30301 gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc gataccagtc
    30361 gctttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca gccgggaata
    30421 ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata gtccagggag
    30481 tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag atagatgaag
    30541 agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta gtaattagga
    30601 ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat gccgaacttt
    30661 actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg
    30721 cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat
    30781 gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata cccgtcgtca
    30841 tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg gtggtctgta
    30901 tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg cagcgagtat
    30961 ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg ccggaatatc
    31021 accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg ggttggtgga
    31081 ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg cgtaactcta
    31141 aaacattgcg cattattttt ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg
    31201 cgttacgggc ctggcactac gacgagaatc attacagtgg cgcaatatgg ctgctctatg
    31261 tcatgatcct ggtatggggc gctgactctg gcgcatatat gtttggcaaa ttgtttggta
    31321 aacataaact ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt atcggtggac
    31381 tcgtgaccgc agcggtaatc tcatggggtt atggcatgtg ggtgaatctc gacgtcgctc
    31441 ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc ggcgatctga
    31501 ccgaaagtat gtttaagcgt gaagcgggaa ttaaggacag cggtcattta attccaggac
    31561 acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc tttgcttgct
    31621 tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt ttctctggga
    31681 tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat ttggtcattt
    31741 ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc
    31801 gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga tcccgttggg
    31861 cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac tccgccacca
    31921 tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag gtccagttgc
    31981 aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg tgcctggcgt
    32041 acgtccggtg gttggtgaaa tagcagccaa ttcgatagct gcggaagcac aaattgcacc
    32101 aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg ccgtgcgttt
    32161 gcagttggtc gataaaattg gcgatgaaag caccaccatt acggtagcgc catttggcag
    32221 cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc ctgataaaga
    32281 agatccggta tcttcgttgg ggattcgtcc tcgtgggccg caaattgaac ctgtactgga
    32341 aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt
    32401 taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg tccgggataa
    32461 cccgggtaaa tccttagcgt tagaaatcga aagacagggg agtcccttgt ctttgacatt
    32521 aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta ttgagccgaa
    32581 agtcattcct ttgcctgatg agtataaagt tgtacgccag tatgggccgt tcaacgctat
    32641 cgtcgaagcc acggacaaaa cgtggcagtt gatgaagctg acggtcagta tgctgggaaa
    32701 attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta tcgccaaggg
    32761 ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg cgcttattag
    32821 cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg ggcatctgct
    32881 gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc aagacttttg
    32941 ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt tcaatgattt
    33001 ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat gaaaaagttg
    33061 ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg
    33121 aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg
    33181 ccggtgcgca caggcgacac ggttaatgat gaagatatca gtaataccat tcgcgctctg
    33241 tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac ccttctggtt
    33301 caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa atcggtgaaa
    33361 gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga atccctcgat
    33421 cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag cgtcggtaaa
    33481 tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa
    33541 ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt tggtaaccat
    33601 gctttcacca ccgatgaact gatctctcat ttccaactgc gtgacgaagt gccgtggtgg
    33661 aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct tgaaaccctg
    33721 cgcagttact atctggatcg cggttatgcc cgtttcaaca tcgattctac ccaggtcagt
    33781 ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg cgatcagtac
    33841 aagctttctg gcgttgaagt gagcggcaat cttgccgggc actccgctga aattgagcaa
    33901 ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa aatggaagat
    33961 gacattaaaa agcttcttgg tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct
    34021 gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc gggtaaccgt
    34081 ttctatgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc tgtcctgcgt
    34141 cgcgaaatgc gtcagatgga aggtgcatgg ctggggagcg atctggtcga tcagggtaag
    34201 gagcgtctga accgtctggg cttctttgaa actgtcgata ccgataccca acgtgttccg
    34261 gggagcccgg atcaggttga tgtagtttac aaggtgaaag aacgtaacac cggtagcttc
    34321 aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg tgtgcaacag
    34381 gataactggt taggtacggg ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag
    34441 acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggcgt aagcctcggt
    34501 ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga ctataccaac
    34561 aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa ctcgctgcgt
    34621 gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt tgcgatgtgg
    34681 cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa cagcttcaaa
    34741 acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg tggttacttc
    34801 ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattccggg atcggataac
    34861 gaatactaca aagtgacgtt agacacggcg acttatgtgc cgattgatga cgatcacaaa
    34921 tgggttgttc tgggtcgtac ccgttggggt tatggtgatg gtttaggcgg caaagagatg
    34981 ccgttctatg agaacttcta tgccggtggt tccagcaccg tgcgtggctt ccagtccaat
    35041 accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga tccggactat
    35101 gattacgaat gtgcgactca ggacggcgcg aaagatctgt gtaaatcgga tgatgctgta
    35161 ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc gtttattagc
    35221 gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac cgtttgggat
    35281 acaaactggg attccagcca atattctgga tatccggact atagtgatcc aagcaatatc
    35341 cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt ggtgttctcc
    35401 tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca gtttaacatc
    35461 ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc gatgacttta
    35521 ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt gcaaatggga
    35581 tggtaaggag tttattgtga aaaagtggtt attagctgca ggtctcggtt tagcactggc
    35641 aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc tgttccagca
    35701 ggtagcgcag aaaaccggtg tttctaacac gctggaaaac gagttcaaag gccgtgccag
    35761 cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa
    35821 agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc agacttttgc
    35881 tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa
    35941 actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg atatcgatct
    36001 ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca tcactgccga
    36061 cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt tagcgcagca
    36121 gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg cgtccatgca
    36181 atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg agcatttagg
    36241 cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc
    36301 cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa ttttagatac
    36361 cacgccgcag cccgcgcaga acattgcacc cagtgcggtg attgacgcga cggcgaagct
    36421 gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg aactgggcga
    36481 taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc
    36541 gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga attgcctgat
    36601 ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc gtggtaactg
    36661 ggtgaagatc ccacagattg gtcgggtaat tattggcgat cgcgtggaga tcggtgcctg
    36721 cactactatt gatcgtggcg cgctggatga cactgttatt ggcaatggtg tgatcattga
    36781 taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcag ttgccggtgg
    36841 cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg gagccagcgt
    36901 aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg gtatggtgat
    36961 gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt
    37021 ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc gtctgaaatc
    37081 gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc caaactttac
    37141 ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt cttatatttt
    37201 gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga gattttagaa
    37261 cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt tgaagaaggt
    37321 cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca gggccatttc
    37381 cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca ggcaacaggt
    37441 attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta cttcgccggt
    37501 attgacgaag cgcgctttaa gcgcccggtc gtgcctggcg atcaaatgat catggaagtc
    37561 actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct ggtcgacggt
    37621 aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt
    37681 gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc gattggcgcg
    37741 aacgctcaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc
    37801 gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga taatgagatt
    37861 tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg cgaaccgacc
    37921 cgcgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca
    37981 gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat caacgcgcac
    38041 attgcgcacg attgtacggt aggtaaccgc tgcatcctcg ccaacaacgc aacgctggcg
    38101 ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt ccatcagttc
    38161 tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca ggacgtccct
    38221 ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat cgaagggctg
    38281 aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta taagctgatt
    38341 tatcgtagcg gtaagacgct cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca
    38401 tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg tctgattcgt
    38461 taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc tccggcgata
    38521 tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg
    38581 gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa atggaagaac
    38641 tggcagtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttacta catattcgtg
    38701 ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt attgatgcgc
    38761 ctgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa accattcatt
    38821 acgtcagtcc gtccgtctgg gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca
    38881 ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa tacaacgtac
    38941 cgtgccgctt tatcggtcat accatggctg atgccatgcc attagatcca gataaaaatg
    39001 gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg ttgccgggca
    39061 gccgtggtgc agaagtcgaa atgcttagtg ccgatttcct gaaaacggcc cagcttttgc
    39121 gccagacata tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa cgccgtgagc
    39181 agtttgaacg catcaaagct gcagtcgcgc cagacctgtc ggttcatttg ctggatggaa
    39241 tgggccgtga ggcgatggtc gccagtgatg cggcgctact ggcgtcgggt acggcagcac
    39301 tggagtgtat gctggctaaa tgcccgatgg tagtgggata tcgcatgaag cctttcacct
    39361 tctggctggc gaagcgactg gtgaaaactg attatgtctc gctgccaaat ctgctggcgg
    39421 gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa ttggctgcgg
    39481 cgctgttacc gctgttggcg aacgggaaaa ccagccatgc gatgcacgac accttccgcg
    39541 agctgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc gttctggagt
    39601 tagcacaatg atcgaatttg tctatccgca cacgcagctg gttgcgggtg tggatgaagt
    39661 cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg acccggcgcg
    39721 cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc tggcgctctg
    39781 tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac ctcacgaaat
    39841 cgacgaactg aatattttac atgcgaccat gctggcgatg cagcgtgccg tcgctggact
    39901 gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat taccgatgcc
    39961 ttcgatggct gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg cgtctatcct
    40021 ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc cgcaatatgg
    40081 ttttgcccaa cacaaagggt atccaaccgc ttttcatctg gaaaaactgg ctgaacacgg
    40141 cgcgaccgaa caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg gacttgcgtc
    40201 ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt
    40261 acacctgcgg gtgcacagtg actactcgat gatcgatggc ctggccaaaa ccgcaccgct
    40321 ggtaaaaaag gcggcagcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct
    40381 ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc ccatcgtcgg
    40441 ggcggatttt aacgtccagt gcgacctgct gggtgatgag ttaactcacc tgacggtgct
    40501 ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag cgtatcagcg
    40561 cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat taaacgaagg
    40621 gttgatcctt ctttccggcg ggcgcatggg cgacgttgga cgcagtcttt tgcgtggtaa
    40681 cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt
    40741 tctcgagctg atccgcaccg gcaggccgga cgaagaaagc tatctgcacg ctgcggtgga
    40801 actggcggaa tcacgcggtt tgcccgttgt ggcgaccaac gacgtgcgct ttatcgacag
    40861 cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga
    40921 tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag aggagatgtg
    40981 cgagcttttt gccgacattc ccgaagccct tgccaacacc gttgagatag ccaaacgttg
    41041 taacgtaact gtgcgtctgg gtgaatactt cttgccgcaa ttcccgaccg gggacatgag
    41101 taccgaagat tatctggtca agcgtgcaaa agaaggcctg gaagagcgtc tggcattttt
    41161 attccctgac gaggaagaac gtcttaagcg tcgtccggag tatgacgaac gtctggatac
    41221 tgaacttcag gttatcaacc agatgggctt cccgggttac ttcctcatcg ttatggaatt
    41281 tatccagtgg tcgaaagata acggtgtacc ggtagggcca ggccgtggtt ctggtgcagg
    41341 gtcactggtg gcctacgcgt taaaaatcac cgatctcgat ccgctggaat ttgacctgct
    41401 gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg ttgacttctg
    41461 tatggagaaa cgtgaccagg ttatcgaaca tgtggcggac atgtacggtc gcgatgcggt
    41521 atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg acgtaggccg
    41581 tgtattgggg catccgtacg gatttgtcga tcgtatctcg aaactgatcc cgcccgatcc
    41641 gggtatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc
    41701 ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa
    41761 cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattactg attttgcgcc
    41821 gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa gcgacgttga
    41881 atacgccggg ctggtgaagt tcgacttcct cggtttgcgt acgctcacca tcatcaactg
    41941 ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagcccccgc tggatatcgc
    42001 cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg aaaccacggc
    42061 ggtattccag cttgaatcgc gcggcatgaa agacctgatc aagcgtctgc aacctgactg
    42121 cttcgaagat atgatcgcac tggtggcact gttccgcccc ggcccgttgc aatcagggat
    42181 ggtggataac tttatcgacc gtaaacatgg tcgcgaagag atctcctatc cggacgtgca
    42241 gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca tcctgtatca
    42301 ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg gcgcggatat
    42361 gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc gttctgtatt
    42421 tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct
    42481 ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tcggcggcct atgctttggt
    42541 gtcatatcaa acgttatggc tgaaggcaca ttatccggca gagtttatgg cggcggtaat
    42601 gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt gctggcggat
    42661 ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc acgtcaacga
    42721 cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg gtaggtgaag gcccgattga
    42781 ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg atctctgcgc
    42841 ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca tgtccggggc
    42901 gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggcg atgcgttaaa
    42961 agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct
    43021 ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt ggccggagca
    43081 ggtggtgtta gatggggaac gtgaaacgtt aggtctgtac ctcacgggac accctatcaa
    43141 ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag acatgcaccc
    43201 gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc gggttatggt
    43261 caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga
    43321 agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat
    43381 acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgactgc
    43441 tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc
    43501 gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt ctctggaacc
    43561 ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg cacgcgcgcg
    43621 attgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa acgatctccg
    43681 tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga atactatgag
    43741 tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa tcgattctct
    43801 gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag aagtgcatcg
    43861 tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg gtgcatggca
    43921 gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg ttcgcctggc
    43981 atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata aagctatcgt
    44041 cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg
    44101 tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag gttaccgcaa
    44161 agcactgcgt ctgatgcaaa tggctgaacg ttttaagatg cctatcatca cctttatcga
    44221 caccccgggg gcatatccgg gcgtgggcgc agaagaacgt ggtcagtcgg aagccattgc
    44281 acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga
    44341 aggtggttct ggtggtgcgc tggcgattgg cgtgggcgat aaagtgaata tgctgcaata
    44401 cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga agagcgctga
    44461 caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga aagagctgaa
    44521 actgatcgac tccatcatcc cggaaccgct gggcggtgct caccgtaacc cggaagcgat
    44581 ggcggcgtct ctgaaagcgc agctgctggc ggatctggcc gatctcgacg tgttaagcac
    44641 tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggctacg cgtaatcggc
    44701 aaatgttctg aaaagggtca cttcggtggc ccttttttat cgccacggtt tgagcaggct
    44761 atgattaagg aaggattttc caggaggaac acatgaacat cattgccatt atgggaccgc
    44821 atggcgtctt ttataaagat gagcccatca aagaactgga gtcggcgctg gtggcgcaag
    44881 gctttcagat tatctggcca caaaacagcg ttgatttgct gaagtttatc gaacataacc
    44941 cacgaatttg cggcgtgatt tttgactggg atgagtacag tctcgattta tgtagcgata
    45001 tcaatcaact taatgaatat ctcccgcttt atgccttcat caacacccac tcgacgatgg
    45061 atgtcagcgt gcaggatatg cggatggcgc tctggttttt tgaatatgcg ctggggcagg
    45121 cggaagatat cgccattcgt atgcgtcagt acaccaacga atatcttgat aacattacgc
    45181 cgccgtttac gaaagccttg tttacctacg tcaaagagcg gaagtacacc ttttgtacgc
    45241 cggggcatat gggcggtacc gcatatcaaa aaagcccggt tggctgtctg ttttatgatt
    45301 ttttcggcgg gaataccctc aaggcggacg tctctatttc ggtcaccgag cttggttcgt
    45361 tgctcgacca caccgggcca cacctggaag cggaagagta catcgcgcgg acgtttggcg
    45421 cggaacagag ttatatcgtt actaatggaa catcgacgtc gaacaaaatt gtgggtatgt
    45481 acgccgcgcc atccggcagt acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc
    45541 atctgttgat gatgaacgat gtagtgccag tctggctgaa accgacgcgt aatgcgttgg
    45601 ggattctggg tgggatcccg cgcggtgaat ttactcgcga cagcatcgaa gagaaagtcg
    45661 ctgccaccac gcaggcacaa tggccggttc atgcggtgat caccaactcc acctatgatg
    45721 gcttgctcta caacaccgac tggatcaaac agacgctgga tgtcccgtcg attcacttcg
    45781 attctgcctg ggtgccgtac acccattttc atccgatcta ccagggtaaa agtggtatga
    45841 gcggtgagcg tgttgcggga aaagtgatct tcgaaacgca gtcgacccac aaaatgctgg
    45901 cggcgttgtc ccaggcgtcg ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta
    45961 acgaagcctt tatgatgcat accaccacct cgcccagtta tcccattgtt gcttcggttg
    46021 agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg gctgattaac cgttcagtag
    46081 aacgagcgct gcattttcgc aaagaggtcc agcggctgcg ggaagagtct gacagctggt
    46141 ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga atgctggcac gttgcgcctg
    46201 gcgaacagtg gcacggcttt agcgatgcgg atgccaatca catgtttctc gatccggtta
    46261 aagtcactat tttgacaccg ggaatggacg agcagggcaa tatgagcgag gaggggatcc
    46321 cggcggcgct ggtggcaaaa ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc
    46381 cttataacct gctgtttctc tttagtattg gcatcgataa aaccaaagca atgggattat
    46441 tgcgtgggtt gacggaattt aagcgctctt acgatctcaa cctgcggatc aaaaatatgc
    46501 tgcccgatct ctatgcagaa gatcccgatt tctaccgcaa tatgcgtatt caggatctgg
    46561 cgcaagggat ccataagctg attcgtaaac acgatcttcc cggtttgatg ttgcgggcat
    46621 ttgatacctt gccggagatg atcatgacgc cacatcaggc atggcagaga cagattaaag
    46681 gcgaagtaga aaccattgcg ctggaacaac tggtcggtag agtatcggca aatatgatcc
    46741 tgccttatcc acccggcgta ccgctgctga tgccgggaga aatgcttacc gaagagagcc
    46801 gcacggtact cgattttcta ctgatgcttt gttccgtagg gcaacattac cccggttttg
    46861 aaacggatat tcacggcgcg aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa
    46921 aaatggcggg ataacttgcc agagcggctt ccgggcgagt aacgtgctgt taacaaataa
    46981 aggagacgtt atgctgggtt taaaacaggt tcaccatatt gcgattattg cgacggatta
    47041 tgcgatgagc aaagctttct actgcgatat tcttggtttc acgctgcaaa gcgaagtcta
    47101 tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt aatgggcaat atgtgattga
    47161 gcttttctca tttccgttcc cgccggagcg ccccagccga ccggaagctt gcggcctgcg
    47221 tcatctggct tttagcgttg atgatatcga tgcggcagtg gcgcaccttg aaagccataa
    47281 cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa aaacgcttca cgttctttaa
    47341 cgatccggac gggctgccgt tggaactgta tgagcagtaa ggcttgtcat cgccgcattt
    47401 gcccggtaac gtgccgggca ttgctactgt aaaatcgcac catcatgaca ctcacgctca
    47461 atagacaact tctctcctca cgccagattc tggtggcctt tagcggcggc cttgattcta
    47521 ctgtcttact gcatcaactg gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg
    47581 ctatccatgt gcatcacggt ttaagcgcca atgctgatgc ctgggtgaaa cattgtgaaa
    47641 acatctgcca acagtggcag gtgccgctgg tggtcgaacg tgtgcagctt gcgcaagagg
    47701 ggctaggtat tgaggcccag gcgcggcagg cacgttatca ggcatttgcc cgtaccttgt
    47761 tgcccggtga agtgctggtt acagcgcaac atctcgacga tcaatgtgaa acctttctgc
    47821 tggcgctaaa acgcggtagc ggccctgccg ggctttcggc tatggcggaa gtttcggagt
    47881 ttgccggaac acggcttatc cgcccgttgc ttgcccgcac gcggggggaa ctggagcagt
    47941 gggcgcgtca gtatgattta cgctggattg aagacgaaag taatcaagac gattcctacg
    48001 atcgtaactt cttgcgcctg cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg
    48061 ccgaagcgac ggcccgcagc gccgcacttt gcgccgaaca agagagcctg ctggatgaac
    48121 tgctggcgga tgatttagcg cactgccaaa cgcaacaggg aacgttaaag attgcgccaa
    48181 tgctggcaat gagcgatgcc cgtcgcgcgg cgattatccg ccgctggctg gcagggcaga
    48241 atgcacctat gccttcccgc gaggcgctgg tgcggatctg gcaggaagtg gcgctggcgc
    48301 gggaagatgc ttccccctgt ttacgtttgg gcgcgtttga aatccgccgc tatcagtcgc
    48361 aactgtggtg gattaaatcc gttgccgggc aaagcgaaac cattgtgccg tggcagacgt
    48421 ggctacaacc gctggaatta ccggcggggc tgggaagtgt acagcttact gcgggaggcg
    48481 atattcgccc tccgcgtgca gacgaagcgg tcagcgtgcg tttcaaagcg ccaggactgc
    48541 tgcatattgt cgggcgcaac ggtggacgta agctgaagaa aatctggcaa gagttaggcg
    48601 ttccgccgtg gctacgtgac accacgccac tgctgtttta tggcgaaacg ctgattgcgg
    48661 cggcaggggt atttgtgacg caagaaggtg tggctgtagg tgagaatggc gtcagttttg
    48721 tctggcagaa aacgcttagt caagtgaaag ccggataaga cgcatcaaac gtcgcatccg
    48781 gcgaaagtca atcaggactc gctcaccacc accgtaccga tttccgggtg gctaaagctg
    48841 gtaattttat ccagacgcag ctcgcgggtt gcaccagcgg cctcgacgac cagatactcc
    48901 acatttttgc gggagactaa atcgctggcc ttcgcctgca atttttcgcc atctttcagc
    48961 tcaagtgtca gcattaaatg atgctggcag gcgagctcaa gattatcgta atcatcacaa
    49021 ttgattggtt gatacgtatc attcattgac ataatcgctc accagtaagt ttgccgcagc
    49081 gtatgctgct ttttccctga cagcctcaga aagggcgtcg tcggcagcca tttcattcag
    49141 cactttcaaa acgcagccca gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc
    49201 cgcgtaccgc ttgcgtatta actcacaata tttttccaca tgccctcctg tcagcactct
    49261 gacttaaccg tggatgcaag tctaagccta cgaagataaa ctctgtttcg caaggtgact
    49321 ataccacact catttctgca atatcagcgc cgcaactgca cgtattccgt tacaatggcc
    49381 tcctgattcg aaaggagttt tcttatggcg cttaaagcga caatttataa agcgacggtt
    49441 aatgtggccg atctcgaccg caaccagttt ctcgatgcct ctctgacgtt ggcgcgccat
    49501 ccttcagaaa cccaggagcg tatgatgctg cgcctgctgg cgtggctaaa atatgccgat
    49561 gaacgtctgc aatttacccg tggtttgtgt gccgatgatg agccggaagc gtggctgcgt
    49621 aacgatcatt tgggtattga tttgtggatt gagctggggc tgccggatga gcgacggatt
    49681 aagaaagcct gcacccaggc cgcagaagtg gcgctgtttg cctataatag tcgggcggcg
    49741 caaatctggt ggcagcaaaa tcagagcaaa tgtgcgcagt ttgccaatct ttccgtctgg
    49801 tatctggacg acgagcagct ggcgaaagtg agcgcctttg ccgatcgtac catgacgctg
    49861 caggcaacga ttcaggatgg tgttatctgg ttatcggatg ataagaataa tctggaagtg
    49921 aacttaaccg tctggcaaca accttcatga ttgtgatttc ccgacatgtt gctatccctg
    49981 atggcgagtt tgagatcacc gccattcgtg cgcagggcgc gggcgggcag catgttaata
    50041 agacctcaac ggctattcat ctgcgttttg acattcgggc gtccagcctg ccagagtatt
    50101 acaaagagcg tctgctcgcc gccagccatc atttgatcag cagtgacgga gtgattgtca
    50161 ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga agcggcgctg gcccggctgg
    50221 tggcagtgat taaagattta acaacagaac aaaaagcccg aagacctacg cggcccaccc
    50281 gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc
    50341 gcggcaaagt gcgcagcggt cgggagtaaa aagaaggaat ggatggtgaa aaaagcgata
    50401 gtgacagcga tggctgtaat cagccttttt actctgatgg gatgtaataa tcgggccgaa
    50461 gtcgatacgc tttccccggc gcaggctgcc gaactgaaac cgatgccgca aagttggcgc
    50521 ggcgtgctgc cgtgtgctga ttgcgaagga atcgaaacct ctctgttcct cgaaaaagat
    50581 ggaacctggg tgatgaatga acgttatctg ggggcacgtg aagagccttc ctctttcgct
    50641 tcctacggta catgggcgcg aactgccgac aagctggtat taaccgacag caaaggtgaa
    50701 aagtcatatt atcgagcgaa aggggatgca ctggagatgc tcgatcgtga aggtaatccc
    50761 attgaatcgc agttcaacta cacgctggaa ccggcaaaat cgagcatgcc aatgacgccg
    50821 atgaccctgc gtggcatgta tttctatatg gctgatgcgg cgacctttac tgattgcgcg
    50881 actggaaaac gttttatggt ggcgaataac gcagagctgg agcgtggcta cctggctgcg
    50941 cgcggcaacg gcgaaaaacc ggtgttactg tcagtagaag gtcactttac gcttgaggct
    51001 aatccggata ccggtgcgcc gaccaaagta ttagcgcccg atacggcagg taaattttac
    51061 ccgaaccagg attgcagtag tttggggcag taacccgtct tgaggcagaa acaaatgaaa
    51121 tattgcctca tgcgctgcgc ttatcaggcc taaagcatac aatgcaatat tttgaattag
    51181 cacgattttg taggccggat aaggcgttca cgccgcatcc ggcattttgc gcacgaggct
    51241 ggcttttatc actgagtcag tccaacaaag cgacttgctt taatataaat ccctggcttc
    51301 aggtgcccaa tatactgtaa ctgcgtttct ggtttaaatg cagagacatc accactacgc
    51361 atatgcatca gatcggcagg gctaatccag cctgattgcg ccagcgtaag cggatgcccg
    51421 gctttggcaa atgcatccgt gacaaattcc gaacaaaacc acgacttttt gtctccttcg
    51481 ccaacgttac ttaactgcgc tttcgccagg ccgctgacgc actgttggcg aaaatcctcg
    51541 gagaacggat tcagtgagca catctggcga gtcaccataa atggaataaa ttcgacaatg
    51601 ccgcgatagt tataaccgct atctttaatt ttattggcga acgcggtgat ttctgtggct
    51661 tgttgcgggg taaggtccgg gactcgtaag acgaaaagct tatcactgtg cttcatggct
    51721 tttttaaggg aaacaatctg gacgccagcg cctgtcgctt cggcaacgtt attatttccc
    51781 aaatagattg caacgtgact cacagaggaa gtgctgaata cgcggatacc aaatgaggtt
    51841 accccaaggc ttgaggagaa aagcaaatcg ccgggtttga gatctggttc cgttatttct
    51901 tttattgatt gttcggtgaa agagctttga tgctggaatt taacagccca tgttttcgcc
    51961 tcggcattta ccgctgtggc tgatgagtct ggctggctga tatcaacggt acaggccgaa
    52021 agtaaaagaa agcaggggag aagcaggcgg cagtacgcct ttggtttatc cattttatac
    52081 aatccatgta aaaaagggcc ccgaaattca ggaccctttc tggcatcagc ctttaatctg
    52141 tttcaccaga taatcgacga tgtcaccagt cttaattaac tgtttctcgc cgttacgacg
    52201 atatttatat tcgatatcat cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg
    52261 aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc ggtcatccag
    52321 cagcacttcg ataccttgtg cgcgcagttc gctgtacagt ttctcagcaa gttcctgtac
    52381 gcggaaggat ttgtgcatgt tcattggcag aatcgccacc tggaacggcg cgatagcgtc
    52441 aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgctgcta ccacacgcgt
    52501 tacgccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt caccctgtac
    52561 ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac cgacttcgat
    52621 accacgtttg atcagcagcg taccctgacc atccgggctt ggatcgccag ccaccacgtt
    52681 gcggatatca gcaatttccg gggtagcgac atcgcgatcc cagttgatac cgaagtagtg
    52741 tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg tacggtcaat
    52801 caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt taaccacggc
    52861 acgaatttct tcttcggtcg caaaagtcag cgggctggca acctgcggca gtttttctgc
    52921 tttaacttcg ttcagctcgt ggtcaccgcg caccagcagc gcaaccagcg ggaagctact
    52981 gccttcaacg gctttaacca gcagagtctt aaccgttttc tcaatcggca gattgaactg
    53041 ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca tttcctgggt
    53101 agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt tcgctgcgta
    53161 gtcagaagta tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca cctgaaattc
    53221 gtgagaggcg ctaccgccga tagaaccggt atcggcctgt acggcgcgga aatccagccc
    53281 catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt cctgcaggga
    53341 ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg aacgcatgac
    53401 gccaaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga agttcagcgg
    53461 cagttgtttg taagagctca gctcgttacg aatcaggtca gtgataactt cttcatgagt
    53521 tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta
    53581 ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg gcattaacac
    53641 ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt ttttcagaac
    53701 gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga tcatcccggc
    53761 gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga
    53821 gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta gaacaggctc
    53881 aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag
    53941 agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc gtcggtgacg
    54001 cgccagcgaa cgttaaaatc atgcaaccag acggcatagg ttttgcccgt ttcctcacct
    54061 ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag cgttaactgc
    54121 ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa acaaaccgcc
    54181 atttctgccg ctggcgcact ttgcgcatag ctggcactgg catcgggaag cgattcagca
    54241 aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc cagactgccg
    54301 agcttcagaa tcacgctgtc tttatggcaa acaacctctt tcagctctac cagcgacata
    54361 ccaatcgggt tggggcgaaa agtggagcgc gtagcgaaaa cacccattct ggcattaccg
    54421 ccgagacgag gcggacgcac agtcggacgc cagccgcctt ccatcgtttg atgaaagacg
    54481 aaaaggatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc ctggttgtag
    54541 ggagcaatga gatgtagttc gccgttggcg ctttttacca gacctggctg gcgcggaacg
    54601 gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg gaaactgctc
    54661 atttcgccgt gatgttaagc gcagaaccga tacatacagc ctgacgatag caacctggcg
    54721 taccgctggt gacttcgcag ctatgcagta ataccgcatt ggctttcatt ttcgaggcgt
    54781 tgatttgcat ccgcttacgt gcggttggaa tgctcggcgg agagtcctga ttagaggcct
    54841 ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact aattcttctg
    54901 cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt
    54961 tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac atggaacagc
    55021 cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt cctcaatgta
    55081 tgatcaaaac gtcaatattg aatcaggcgc ttgtaaaaat gacaagacgg gcaagcgccc
    55141 gtcctgaatg atattacaaa ttgtggaaac agcctaaata ttaccagcct ttaacagcgc
    55201 cgccgttaaa cactttgttt gctgcttcgt aaacttcgtc agactgataa gcctgaacga
    55261 atttcttcac gttttcggcg tctttgttgt cttcacgcgt tacgatcagg tttacgtacg
    55321 gggactcttt atcttcgaca aagataccgt ctttcgctgg agtcaggcca atctggctgg
    55381 cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagag cgcggcagct
    55441 gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca tcaagaacgg
    55501 tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc agcagcagca
    55561 gtgaacgacc aaggttagtt gggtcgtttg gcacggcaac ctgcgaacca tcctgcagtt
    55621 catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaat gtgttgccta
    55681 ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt ttatgctgga
    55741 aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca tagtcgttga
    55801 aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc gcgacttctg
    55861 caacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga tctttttcat
    55921 cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc gcaaaggttt
    55981 tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt gaacgttact
    56041 tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa accagaatga
    56101 ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag ccatactgat
    56161 agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa taaccgacca
    56221 gggtaatcag ggtaatagtt gccgcattca ccagacccgg caacgcttcc ggtaacagca
    56281 ctttacggac gatctgcatt ggcgtggcac ccattgcgcg ggaggcttca attaacccgg
    56341 ttgggatctc cagaagagcg ttctcgacca tacgggcaat aaacggtgct gcaccaacgg
    56401 ttaacggaac aatcgctgcc tgcaatccaa tcgatgtacc gacaataacg cgggtaaacg
    56461 gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc acaatcgcag
    56521 aaatggtacg atacaacttc gcgttagcaa taatttgccc cggacgcgtg acataaagca
    56581 gaacgccaac cggcagacca atcacaaagc caaaaaaacc ggatacgaag gtcattgcca
    56641 gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata acccagtacc
    56701 tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg
    56761 ccgtgcattt cagtcagcat aatgccgaac ttaacgccac cggcgtaatc catctgcgcg
    56821 ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag tggggcatcg
    56881 accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa tggctccgct
    56941 tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg aataaacttc
    57001 tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg ctcgatcagt
    57061 tcgccattgc tgatgaccgc cacgcaatca caaatgcgct tcacaacgtc catttcgtga
    57121 gtgatcaaca gaatcgtcaa acccagacgg cggttgatgt ctttcagcag ttcgagaata
    57181 gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa tactttggga
    57241 ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga aagattcgac
    57301 gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt cacgcgacgt
    57361 ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt gccaaaaaca
    57421 gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg acgagctttg
    57481 gtcaactcgg attctgacag cgtggtcagt tcctggccat cgaccagcac gctaccctcg
    57541 gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc gcctgaggca
    57601 ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt caacgcctgg
    57661 atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat cattgattat
    57721 ttattatcgt cattaagtta gtcgtggcat ttcgaatgcc tggaacgggc aacgccgtca
    57781 atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc gaagagcact
    57841 ttcttgcagg tcgaaacatg cgatactagc gtcacatgcc ttattaagga gctataaaag
    57901 gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa tgtcgatcac
    57961 ggttatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga cgccatgcgc
    58021 gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg cattgctcgc
    58081 ggtaaattta ctgaagcaca gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg
    58141 gaccgcgatg tcgatctgga tggtatctat tattgcccgc atcatccgca gggtagtgtt
    58201 gaagagtttc gccaggtctg cgactgccgc aaaccacatc cggggatgtt tttgtcagca
    58261 cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa attagaagat
    58321 atgcaggcag cggctgcggc gagcgtggga acaaaagtgc tggtgcgtac gggtaaacct
    58381 attacgcctg aagcagaaaa cgcggcggat tgggtgttaa atagcctggc agacctgccg
    58441 caagcgataa aaaagcagca aaaaccggcg taatgattaa aagatgagcg gttgaaataa
    58501 aaatgcattt ttccgcttgt cttcctgaga cgactcccta taatgcgcct ccatcgacac
    58561 ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat
    58621 tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa
    58681 agccgcgtcg caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat
    58741 acggattctt gacgtcgcaa gacgaa
//
LOCUS       DAPQZD010000009        58446 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPQZD010000009 DAPQZD010000000
VERSION     DAPQZD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.009"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(9..224)
                     /locus_tag="RO454_000577"
     CDS             complement(9..224)
                     /locus_tag="RO454_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDY0932459.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
     gene            complement(287..1759)
                     /gene="der"
                     /locus_tag="RO454_000578"
     CDS             complement(287..1759)
                     /gene="der"
                     /locus_tag="RO454_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDY0932460.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1877..3055)
                     /gene="bamB"
                     /locus_tag="RO454_000579"
     CDS             complement(1877..3055)
                     /gene="bamB"
                     /locus_tag="RO454_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDY0932461.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3066..3686)
                     /gene="yfgM"
                     /locus_tag="RO454_000580"
     CDS             complement(3066..3686)
                     /gene="yfgM"
                     /locus_tag="RO454_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDY0932462.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3704..4978)
                     /gene="hisS"
                     /locus_tag="RO454_000581"
     CDS             complement(3704..4978)
                     /gene="hisS"
                     /locus_tag="RO454_000581"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDY0932463.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5089..6207)
                     /gene="ispG"
                     /locus_tag="RO454_000582"
                     /gene_synonym="gcpE"
     CDS             complement(5089..6207)
                     /gene="ispG"
                     /locus_tag="RO454_000582"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDY0932464.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6234..7241)
                     /gene="rodZ"
                     /locus_tag="RO454_000583"
     CDS             complement(6234..7241)
                     /gene="rodZ"
                     /locus_tag="RO454_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDY0932465.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7526..8680)
                     /gene="trmG/rlmN"
                     /locus_tag="RO454_000584"
     CDS             complement(7526..8680)
                     /gene="trmG/rlmN"
                     /locus_tag="RO454_000584"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDY0932466.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8830..9261)
                     /gene="ndk"
                     /locus_tag="RO454_000585"
     CDS             complement(8830..9261)
                     /gene="ndk"
                     /locus_tag="RO454_000585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDY0932467.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9410..11722)
                     /gene="pbpC"
                     /locus_tag="RO454_000586"
     CDS             complement(9410..11722)
                     /gene="pbpC"
                     /locus_tag="RO454_000586"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDY0932468.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11723..16684)
                     /gene="yfhM"
                     /locus_tag="RO454_000587"
     CDS             complement(11723..16684)
                     /gene="yfhM"
                     /locus_tag="RO454_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDY0932469.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16891..17736
                     /gene="sseA"
                     /locus_tag="RO454_000588"
     CDS             16891..17736
                     /gene="sseA"
                     /locus_tag="RO454_000588"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDY0932470.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18229..19005)
                     /gene="sseB"
                     /locus_tag="RO454_000589"
     CDS             complement(18229..19005)
                     /gene="sseB"
                     /locus_tag="RO454_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDY0932471.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19147..20430)
                     /gene="pepB"
                     /locus_tag="RO454_000590"
     CDS             complement(19147..20430)
                     /gene="pepB"
                     /locus_tag="RO454_000590"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDY0932472.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20489..20689)
                     /gene="iscX"
                     /locus_tag="RO454_000591"
     CDS             complement(20489..20689)
                     /gene="iscX"
                     /locus_tag="RO454_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDY0932473.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20701..21036)
                     /gene="fdx"
                     /locus_tag="RO454_000592"
     CDS             complement(20701..21036)
                     /gene="fdx"
                     /locus_tag="RO454_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDY0932474.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21038..22888)
                     /gene="hscA"
                     /locus_tag="RO454_000593"
     CDS             complement(21038..22888)
                     /gene="hscA"
                     /locus_tag="RO454_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDY0932475.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22905..23420)
                     /gene="hscB"
                     /locus_tag="RO454_000594"
     CDS             complement(22905..23420)
                     /gene="hscB"
                     /locus_tag="RO454_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDY0932476.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23516..23839)
                     /gene="iscA"
                     /locus_tag="RO454_000595"
     CDS             complement(23516..23839)
                     /gene="iscA"
                     /locus_tag="RO454_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDY0932477.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23856..24242)
                     /gene="iscU"
                     /locus_tag="RO454_000596"
     CDS             complement(23856..24242)
                     /gene="iscU"
                     /locus_tag="RO454_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDY0932478.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24270..25484)
                     /gene="iscS"
                     /locus_tag="RO454_000597"
     CDS             complement(24270..25484)
                     /gene="iscS"
                     /locus_tag="RO454_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDY0932479.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25596..26084)
                     /gene="iscR"
                     /locus_tag="RO454_000598"
     CDS             complement(25596..26084)
                     /gene="iscR"
                     /locus_tag="RO454_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDY0932480.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26355..27095)
                     /gene="trmJ"
                     /locus_tag="RO454_000599"
     CDS             complement(26355..27095)
                     /gene="trmJ"
                     /locus_tag="RO454_000599"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDY0932481.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27214..28017
                     /gene="suhB"
                     /locus_tag="RO454_000600"
     CDS             27214..28017
                     /gene="suhB"
                     /locus_tag="RO454_000600"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDY0932482.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28162..29016
                     /locus_tag="RO454_000601"
     CDS             28162..29016
                     /locus_tag="RO454_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0932483.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29207..30487
                     /gene="csiE"
                     /locus_tag="RO454_000602"
     CDS             29207..30487
                     /gene="csiE"
                     /locus_tag="RO454_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDY0932484.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30479..31618)
                     /locus_tag="RO454_000603"
     CDS             complement(30479..31618)
                     /locus_tag="RO454_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDY0932485.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31988..32410
                     /locus_tag="RO454_000604"
     CDS             31988..32410
                     /locus_tag="RO454_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDY0932486.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32458..33330)
                     /locus_tag="RO454_000605"
     CDS             complement(32458..33330)
                     /locus_tag="RO454_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDY0932487.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33342..34436)
                     /locus_tag="RO454_000606"
     CDS             complement(33342..34436)
                     /locus_tag="RO454_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDY0932488.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34469..35467)
                     /locus_tag="RO454_000607"
     CDS             complement(34469..35467)
                     /locus_tag="RO454_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932489.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35470..36999)
                     /locus_tag="RO454_000608"
     CDS             complement(35470..36999)
                     /locus_tag="RO454_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDY0932490.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(37022..38005)
                     /locus_tag="RO454_000609"
     CDS             complement(37022..38005)
                     /locus_tag="RO454_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDY0932491.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38102..41383)
                     /locus_tag="RO454_000610"
     CDS             complement(38102..41383)
                     /locus_tag="RO454_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDY0932492.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41501..42694
                     /locus_tag="RO454_000611"
     CDS             41501..42694
                     /locus_tag="RO454_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDY0932493.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42758..44011)
                     /gene="glyA"
                     /locus_tag="RO454_000612"
     CDS             complement(42758..44011)
                     /gene="glyA"
                     /locus_tag="RO454_000612"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDY0932494.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44340..45530
                     /gene="hmpA"
                     /locus_tag="RO454_000613"
     CDS             44340..45530
                     /gene="hmpA"
                     /locus_tag="RO454_000613"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDY0932495.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45575..45913)
                     /gene="glnB"
                     /locus_tag="RO454_000614"
     CDS             complement(45575..45913)
                     /gene="glnB"
                     /locus_tag="RO454_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDY0932496.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(45974..47308)
                     /gene="glrR"
                     /locus_tag="RO454_000615"
     CDS             complement(45974..47308)
                     /gene="glrR"
                     /locus_tag="RO454_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDY0932497.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRNELDANDFKE"
     gene            complement(47298..48011)
                     /gene="qseG"
                     /locus_tag="RO454_000616"
     CDS             complement(47298..48011)
                     /gene="qseG"
                     /locus_tag="RO454_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDY0932498.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48176..49603)
                     /gene="qseE"
                     /locus_tag="RO454_000617"
     CDS             complement(48176..49603)
                     /gene="qseE"
                     /locus_tag="RO454_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDY0932499.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50179..54066)
                     /gene="purL"
                     /locus_tag="RO454_000618"
                     /gene_synonym="purI"
     CDS             complement(50179..54066)
                     /gene="purL"
                     /locus_tag="RO454_000618"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDY0932500.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54324..55880
                     /gene="mltF"
                     /locus_tag="RO454_000619"
     CDS             54324..55880
                     /gene="mltF"
                     /locus_tag="RO454_000619"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDY0932501.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55877..56413)
                     /gene="tadA"
                     /locus_tag="RO454_000620"
     CDS             complement(55877..56413)
                     /gene="tadA"
                     /locus_tag="RO454_000620"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDY0932502.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56438..57073)
                     /gene="yfhb"
                     /locus_tag="RO454_000621"
     CDS             complement(56438..57073)
                     /gene="yfhb"
                     /locus_tag="RO454_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDY0932503.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57282..58130
                     /locus_tag="RO454_000622"
     CDS             57282..58130
                     /locus_tag="RO454_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDY0932504.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58186..58446
                     /gene="yfhL"
                     /locus_tag="RO454_000623"
     CDS             58186..58446
                     /gene="yfhL"
                     /locus_tag="RO454_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDY0932505.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13816 a        16294 c        14563 g        13773 t
ORIGIN      
        1 aagtgatatt aagcaataac aaattcaacg cgttttttag atattaaacc gtgcccatgt
       61 tggcagaaat aatccacagc accacaagct tttaacactt gtaacggctg atgacactca
      121 gggcaaagtg cctggacggt aaagtctttg tcgcaagttg cacagtgagc agtctcgcca
      181 ttgcgttcga gaggggtttt gcaggttgga cagttgagtg ccataaatac tcctcgcgtg
      241 ttattcggtt tccctctccg tgtggaaagg gaaaccagat aagtgattac ttacttttct
      301 tgatgtgctt catcagacgc ttacgcttgc gcatctgagt cggcgtcagg gtattacgct
      361 tgttcgcata cgggttttcc ccttctttga actgaatacg aatcggcgat cccattacgt
      421 ccagggattt gcggaagtag ttcatcaggt aacgcttgta ggaatcaggc aggtctttca
      481 cctgattgcc gtgaatcacc acaatcggcg ggttataacc cccggcgtgg gcgtatttca
      541 gcttcacacg acgaccgcgt accagcggcg gttggtgatc ttcaacagcc atcgtcatga
      601 tgcgcgtcag catagaggtc cccacacgac gggtggagct gtcatacgct tcacgtactg
      661 attcaaacaa gtttccaaca ccactgccgt gcaaggcaga gataaagtgc acacgagcaa
      721 aatcgataaa gcccagacgg aagtccagcg tctctttcac ctgctctttc acttcctggc
      781 tcaggccgtc ccacttgttc actacaatca caagtgagcg cccactattg agaataaagc
      841 ccagcaagga gagatcctga tcggaaatac cttcgcgcgc atcaatcacc aacatcacca
      901 cgttggcatc ttcaatggcc tgcaacgttt tgattaccga gaatttctct accgcatcgg
      961 tgattttgcc gcgtttacgt acgccagcgg tgtcaatgag cacatactca cgtccgtcgc
     1021 gttccattgg aatgtagata ctgtcacgcg tcgtgccagg catgtcgtaa acaacaacgc
     1081 gctcttcacc aagaatacgg ttagtgagtg tagacttacc tacgttcggg cgacccacaa
     1141 tcgccagttt gatcggcaga ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt
     1201 tctcttccgc ttcaaattgc gcccagtatt cggcgtcttc gtcgacttct tcttgcggtg
     1261 cgagatcttc catccacggc agcagcacgt gctccagcag acttaatacg ccacgaccgt
     1321 gagacgcggc gatcgggtag atttcaccta aaccaagcgc gtagaaatca accactgcct
     1381 gatcgggatc cagaccgtca gttttgtttg ccaccaggaa ggtcggtttt tcacgggagc
     1441 gcagatgttt agcaatcgct tcatccgccg gcatcaggcc cgcgcgcgca tccaccataa
     1501 acagtacgac gtccgcttct tcaatcgcca gcagcgactg ttccgccatg cgggtttcta
     1561 caccgtcttc tgtgccatca atcccgccgg tatcaatgca gataaactca cggccttcaa
     1621 tttccgcacg accgtactta cggtcacgag tcagacccgg gaaatccgca accagcgcat
     1681 ctcgggtgcg agttagacgg ttaaataacg tggattttcc tacgttaggg cgcccgacaa
     1741 gcgcgaccac aggtaccatg tttaaagcct catttttata aatcatcaga caacgcacgc
     1801 tatattcgtg tcgttgttaa aaacaggaaa acggcccctg tccaggagcc gttttcaaag
     1861 tgaacgacag agacgattaa cgtgtaatag agtacacggt tccgtctttt gcctggatca
     1921 gcagtttgcc gtcagcagca accggttcag tctggaaacc ggaactatca actttttgct
     1981 gggcaacgaa acgaccatct tcgacgttaa tccagtgcag ataaccttca ctgtcgccta
     2041 ccaccaggtt gccattatac agcaccggag aagtcagcag gcgatgcagc agatcgcttt
     2101 gtgtccacag cgtaacgccg ccatcaatgg tcaacgccat cacccggtca ttttgatcga
     2161 ccagatagat gcgattgccg tcgacgatga aatcattcac cgaacccagt tcgcgtttcc
     2221 acataatctg accactgcgc agatcaagcg ccgtcaggtt accattatag gccagcgcga
     2281 aaacaacgcc gttaacaacg acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg
     2341 tagaaccggt cgcctgggaa atacgctgct gccaaatcat ctggccctgt tccatcagca
     2401 ctgcgctgac gcgaccatta tcgccaccca cgacggccgc accaaaagcc gttgccggcg
     2461 cagactcgcc acgcaaagag agcgaaggca tatcgaggtt aactgtccat ttgacagcgc
     2521 cgtcagcttc gttcagcgct tgtaactgac cgttactggt gtggattaac accagaccgt
     2581 cgctgaccac cgggcgcgaa agtgcttcac ccgcgacttt agtttgccat gccacagtac
     2641 catcgctggt attcagcgcg taaacctgcg ccttttcggt gccaatgtag acatgcccac
     2701 cagacacggt cacgccgcca gaaagtaatg caggatcttt agagaaccag ccatctttct
     2761 cggccaggtt gacagaccag atttctttgc cgtcatccgc attcagcgct tttactaaac
     2821 cagcgcggtc cgctgcatag acaacgttgt ccgccagtgc cggatgaaga ttggaataga
     2881 agttgccaat gccgctacca acggaagtgc tccacacagt agtcggcgta aactggtttt
     2941 caacggttgg caatggggac atctttacca catcttcttc gctgttaaac agcgaacagc
     3001 cgcttaaaag ggtaacggaa agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt
     3061 ccctctcaga tggacaaatt attaattttc atctgcatca tttcgctcag tgccggggta
     3121 acatcgcttt tcacgcctgc ttcccatgca ctacgcgcac cttgcttatc acctttgctc
     3181 agcaatgctt caccacgcag gtcggcaaca atggcagccc acccttcacc tttgatagca
     3241 tcaagggttt ttagcgcggc atcagcctgc ttgagctgta cctgaacgcg agcaagacgc
     3301 agatttatca cggctttgag attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg
     3361 gcggcagctt tctctagttc atttttgtca acaaattgct gcgcaagttc caaagaagcc
     3421 agcgcaccat aagtattttt attttcagca gcaaattttt ccgccgctgg gatgctatcc
     3481 ggtttgcctt cgctcactgc ggtaaccgca ttttgatagg caagagaagc ggagcgtgca
     3541 gaatcaacct gatggctatt ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc
     3601 accccaacag ccagtgcttt gccattttca gcaaaaaagc gtttaaccgc ttcaacctgg
     3661 tcgttttcgt tctcgtaaat ttccacgctg tccttctcct tccttaaccc agtaacgtgc
     3721 gcaaatgcgc ggctacgcta tcctgcgcaa ctgcagtttg ctcaccagag cgcaaatcct
     3781 tcactactgc tgtgccgtta gccacttcag actcacccag caccacagca acgcgggcac
     3841 cccatttatc agcacgggca aactgtttct taaagttgcc gccgccgtgg ttggtcatca
     3901 gtttcacacc cggtaattca tcacgcagac gctctgctaa tgccatagcc gcagattgtg
     3961 tgtcagcacc tgaagccacc aggtatatat cgacaacagg atcggcttta aattccggat
     4021 taacggcctg aactaacaat acaagacgtt cgaggcccat cgcaaaaccg actgccggtg
     4081 ttgcacgacc acccagttgt tccaccagac cgtcataacg accacctgca cacacggtgc
     4141 cctgggaacc gaggctgtta gtcacccact cgaaaacggt acggttatag taatccagac
     4201 cgcgcaccag acgctggttt acagtgtaag cgatccccgc gctctcaagc agtttgcaca
     4261 gaccggcaaa gtgctcacga gattcctcgt ccagataatc gcctaatgcc ggagcgtcgt
     4321 tgagaagcac ctgcacttcc ggattttttg aatccagtac gcgcaacggg ttagtgtaca
     4381 tgcggcgttt gcagtcttcg tccagctttt ctttatgctg ctcaaggaat gccaccagcg
     4441 catcgcggta attcgcacga gcttccagcg aaccgataga gttcagctca agagttacgt
     4501 gctcggaaat acccagcgcg cgccaccagc gggcagtgag cataatcagt tcggcgtcga
     4561 tatccggacc ttgcagaccg aaaacttcgc agcccaactg atggaactga cgataacgcc
     4621 ctttctgcgg acgctcgtga cggaacatag gcccgatata ccacagacgc tgttcctgat
     4681 tgtacagaag accatgctcg atgccggcgc gtacacagcc cgccgtccct tcagggcgca
     4741 gagtcaggct gtcgccattg cgatcctcaa aggtgtacat ctctttttca accacgtcgg
     4801 tgacttcacc aatcgcacgt ttgaatagcg gggtctgctc tacaatcggc aagcggattt
     4861 cactgtaacc gtagctgccg agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg
     4921 ccgtttcgcc aggcaggtaa tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt
     4981 ttattctctt tctgaatata aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc
     5041 atgcgggttc aatcatacac gggaagcgag gcgcttccca tcacgttatt atttttcaac
     5101 ctgctgaacg tcaattcgac gcgcttcgtc cagctgactg gctttcgcac gaatgcgcgc
     5161 ttccagctga tcgatcatat cgttgttgtc cagacggtct ttgcgcacgc catcttcata
     5221 gaggccgctt ttcttgttgc cgccagtgac gccgagtgta gaaaccagcg cctcacctgg
     5281 gccattcacc acgcagccga taatcgaaac gtccatcgga gtgattatat cttccaggcg
     5341 ttgctccagc gcgttaaccg taccgataac atcaaattcc tgacgcgaac aggtcgggca
     5401 ggcgatgaag ttgatcccgc gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac
     5461 tttgatctct tcgaccggat cggccgccag cgatacgcgc agcgtgtcgc cgatgccttc
     5521 agacagcagc agacctaaac caatagcgga ttttactgcc ccgctgcgcg caccaccggc
     5581 ttcggtgatc cccagatgca gcggctgatc gatttgtttt gccagcaaac gataagactc
     5641 aacagcgagg aagacgtcag acgctttcac actgactttg aactgatcga agttcaggcg
     5701 atcgagatga tcaacatgac gcatggcgga ttccagcaac gcctgcggcg tcggttcgcc
     5761 atacttttct tgcagatctt tttccaacga tccggcgtta acgccaatac ggatcggaat
     5821 gtttttatcg cgcgcacagt caaccaccat gcgaatacgc tcttcattac cgatattgcc
     5881 agggttaata cgcagacaat cgacgccgta ttccgctact ttcagcgcaa tgcgatagtc
     5941 gaagtggatg tcagccacca gcggcacgtt aacctgctgt ttgatgagtt tgaacgcttc
     6001 tgccgcgtcc atcgtcggta cggagacacg gacgatatca gcgccaacgc gttccagcgc
     6061 tttgatttga ttgaccgttg cttcgacgtc tgtcgtacgc gtattggtca tggactgtac
     6121 ggcgatggga gcaccatcgc caatcggcac attcccaacg taaatacgtg ttgattttct
     6181 acgttgaatt ggagcctggt tatgcatgaa aaatctcccg cgttacccgt ctgttactgc
     6241 gccggtgatt gttcggcatt gagggtcaga cgcgcaacct ggttagttct gataaaacga
     6301 ctcagatcga caggtttccc ttgatactgg atctgtactg cggctggcgc accaattttc
     6361 agtttgtacg gtgcctggcc ggttaagttc aaattaccgt ctttacgctg cataccgcta
     6421 aacagttttt taccggtagc atcggtgact tccagccagc aatcggaagt aaagttcatc
     6481 accagcgcat tcgggtcaac cgccggcgtg gtcacgccag cctgatcggt tggcaacggc
     6541 gcagcgccat ctggtgttgt tgttgccgct ggtgccggag tcgcagcggt atcaacattt
     6601 gcctgcgaag gtggaacaac cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt
     6661 acggcaggtg tttgcgtgtt ggttgcggtg gtatccacag gtgatgtcgg cgtggtcgcc
     6721 atagcctgat ctgtggttgt cgacgtatct aacggtacac tctggctttg attattattc
     6781 agttccgcag aagattgatc ggccatagtg gtgatctctt cctgctgagc tttgtggtct
     6841 tgccaccacc aggcaccgct cagaccaata accacaaaca acaccagcca ggtgaaggtc
     6901 atcagccagc cgtcacgttt tttgcggcgt ttaccgaggg aaaaactctg catcggcgca
     6961 acttttgcag cccgtagtgg agcctgcttt tccagccctg gcagcagttc ttcttctgga
     7021 atatgtacca gacgcgcata agagcggata tatccgcgca ggaatgttga agcaagatcg
     7081 gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg
     7141 gcaacggcct gttgactaag tcctagttgt tcgcgagcat tacgcaggcg agcgccggta
     7201 gtaagtgctt cattttggtc gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa
     7261 atgagaattg agatgccggt gaaccatcat gctcacccac accgcgaaac atccgttaag
     7321 ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc cacattcgct
     7381 aaggctaact tactgttgca tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt
     7441 aatattgacc agacaattgc aacttaatac ttcattcata aaagattacg ccacggtaca
     7501 taaagtaacc gtggcgtaat ggctatcaga ccgctttaat gtcgatggct tcaccctgca
     7561 tccgtttacg cagggtacgt ttcgtacggt cgataacatc gcccgccaac tgaccacagg
     7621 cagcatcaat gtcatcacca cgagttttac gcacaatagt ggtgaaaccg tagctcatca
     7681 gcacttttga gaaacggtcg atacggctgt tcgaactgcg tccatacggc gcgcccggga
     7741 acgggttcca cgggatcagg ttgatcttac acggcgtatc tttcagcagt tctgccagtt
     7801 ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca atagtgactc
     7861 ggccctgatt ggcgttggat ttctccagat aacggcgcac cgccgcaagg aacgtttcga
     7921 tattgtactt tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca
     7981 gggaaattgc cagcgcaacg tcgatcatat cgccgagttt atccagcgcc ggaaccacac
     8041 cggaagtgga aagcgtgacg cgacgttttg acaggccaaa accgaaatca tcaagcatga
     8101 tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg ctcgcccata cccatcatca
     8161 ctacgttagt gattgggcgc tgaccggtga cttttgctgc gccgacaatt ttcgccgcac
     8221 gccacacctg gccgataatt tccgataccc gcaggttgcg attaaagccc tgctgggcgg
     8281 tggaacagaa tttacactcc agcgcacacc ccacctgcga agagacgcag agcgtggcac
     8341 ggtcgtcttc cgggatatac accgtttcaa cgcgctgatc gccaacggcg atcgcccatt
     8401 taatggtgcc gtcagatgaa cgctgttctt caaccacttc cggtgcacgg atttccgcca
     8461 cctctttcag tttgccgcgc aacactttat tgatgtcggt catctcatca aagttgtcgc
     8521 agcaatagtg atacatccac ttcatcacct gatcggcgcg gaagggtttt tcacctaaat
     8581 ctttaaaaaa ctcccgcatc tgctgacggt tgagatccag caggttgatt tttccatctt
     8641 tcgtggtgac gttttcaggt gtgactaatt gttcagacat atgctattcc ggcctcgtta
     8701 ttacacgtta tggcccctgg agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt
     8761 ccgggggcgc tgcattgtac aaattctggc gcacggatgc cacgtttgca cgcggcattt
     8821 acgaaattat taacgggtgc gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc
     8881 gcgagcggca gattcgacgg aatcagaacc gtgggtacca ttttcggtca ggctgtcagc
     8941 gtaatcagca cgcagagtac cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc
     9001 gcggtgacgc tgaacagcat tttcaccttc cagcacggaa acaacgatcg ggccagaagt
     9061 catgaattca accagaccat caaagaacgg ttttccatcg tgttcagcat aaaagccacg
     9121 agcctgttca acggtcaggt gcagcatttt ggtgccgaca attttgaacc ctgcagcttc
     9181 aaagcgcgca aagatactac caatcacgtt ttttgctact gcgttcggtt tgatgatgga
     9241 aaaagtacgt tcaatagcca tttttacctc tgtaaattgt tctgttgttg tctgtaccag
     9301 cgtacgagat ggcgcggatt ataatgagca acagggccgt tgactattga tgaaggtaac
     9361 atttttttaa aataaaccca gtttaagcaa caatcaggga caaaacagac tattgcatga
     9421 caaatttcac tgtcgctatt tgccccacgt catccatcac cagcaattga taatcgcctt
     9481 tatccgtcaa atgcagggtc acgttgcgcc cacgttcagt taacggttcg ccgttcagaa
     9541 accaccagcg ttcacctgcc cctccacttg attgcaacgg caaagtcgct tccgctgcgc
     9601 ccggtaaacg tttaataatc gcgccatcgc gcacacctgt tagctgcagc gggagttgtg
     9661 catcgtgacc gtatggcggg caaattgtcg aggccggagg caagcgcaca gcgcgacgtt
     9721 ctgatgcggg cagccagggt tccagcggta gcggccagac atttatcatt tcttgtcgcg
     9781 cctgagggca atcagcggca acacgtttgc cgttttcatc cagccagatg gggaaacgaa
     9841 tgccattaat gccttcctgc tccggcagta atagagttgg cggctggctc ccgtccagca
     9901 gccaggttgc caggcggcgg cgacagttac cgtcaccttc cggcaaagac tgtccgcccg
     9961 gccagcagat aacgccacga cttacagagt caggacgtgg gtcttccggc aaattcacac
    10021 tgcgcgacag taagatatta ttaacctgat tcaacagcgg tacggcactg gcaaagccaa
    10081 actgaccaac aacgggcgtg ccgtccggtc tgccagtcca gatcccaatg acatagcgag
    10141 cgttaacccc aatcgcccag gcatcacgat agccatagct ggtgcccgtt ttccatgcca
    10201 gtggggcgac gcgcggcaag gcaccatccg gcaagggttg cgcttcatca gccataatcc
    10261 ggcgaatgat ccacgcagcc cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag
    10321 gctgtaagcg caatttgcct gccttgccgt ggcgagcaaa cgcggtatac gctgctgcca
    10381 tatcttccag ttttgcgcca gcgccgccga gaatgagcga aagattcggc gcagcaccgt
    10441 tgggcagata caacggcaat ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat
    10501 aggcttccag cacctgcaca gcaggtaagt tcagcgatcg caccagcgcc tcactcatgc
    10561 tgatcgggcc atgaaaaccg ctgtcaaagt tgccaggtcg gtaatcaccg gtgcgtcggg
    10621 ggacgtcttg caacagcgat gccgggtgga tcacgccttc atccagcgcc agaccataag
    10681 caaacggttt gagcaccgat cctggcgatc ggatcgcatt gaccatatca acatgaccaa
    10741 agcgtgaatc atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc atatcggtat
    10801 gatcaaccac gatcatcgcc agtgagctgc gcggtggcaa tcgtcctttc cagttttgcg
    10861 ccagttcttc cagacgtcgt tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt
    10921 tgcttttacc gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg
    10981 ccagccagat gggttcttcc cttgactctt ttacccgctc acgggaccac acaccttgca
    11041 cggccatccg ttcgagcact ttattacgcg cggcttcggc acgctccggc caacgatccg
    11101 ggcgaagacg gctgggcgct tgcggcaaca ccgccagcat tgccgcctcg gaatagctta
    11161 aattcgcagg cgatttcccg agataagccc aacttgccgc accaatcccc tgcaacgtac
    11221 cgccaaacgg ggcacggtta agatacaagg tcagaatttc acgtttagac agatgccatt
    11281 ccagttgcaa cgcgcgccag agctgacgaa ttttgccgcc aaacgttttg gggtgaggat
    11341 caagcaggcg cgccacctgc atggtgagcg tgctgccgcc ggaaatgacc cgacccgacg
    11401 tgagatcttg ccaggctgca cgcgccaccg agaacggatt cacccccgga tgcttccaga
    11461 accagcgatc ttcataattg atcagtgctt caaggtaacg tggagaaaca tcttcgattg
    11521 ttaccggata acgccagatg ccgtcagcat cggcgaaacg ccagagtggc gtaccatcct
    11581 gcgccaccac cacgcgtgca ggattcactt cttgcaatgg cagaggccag agtttatctg
    11641 ctgcccatgc tgccagaatg atgataaaag gcgcggctgt cagcattatc cagcagccgc
    11701 gtttggttaa caggcgaggc atttacggtc tgactatcaa cagatcgtca gccgcaccag
    11761 tcgcccgcca ttgaggaaca tacattgatt ccaccatagg ctgcggcacc tgatacgttc
    11821 ccggcgtcac cgcccgcgcc agatacacca gcgttaccgg ttgatattca tcaacggcaa
    11881 ccgccgccac aaagcgatcg tcacggaact ctatgtgctt aatgctcgcc tgctgcatct
    11941 ggttcagcaa gttctgcact tcaccaccac tttgctccag gctggcgccg ccgttcgcca
    12001 gattctggtt ttccagttcc agccccgcag gcaacagatc cacgactaac gcatccggta
    12061 cactgttact ggctttaacc tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt
    12121 ccagcgattt gctcttaccg tcagtaccca gaatatgacg ctcaatttgc agcacattgc
    12181 tcgccggtaa aggtgcggat tgcggataac cgctggcatc cacacgcagc cataacggct
    12241 gatcgccact gttggtcacc tgcaaggtgg caagttgatc gctattcaga ttgctggttt
    12301 gcgctttctc gcctgtcagc ggctcagcag agaaagtggt ttgcgcctgc catttgcccg
    12361 gtaaatcctg aagagtacgg gcagccagga acaacgcgtt actttcctgc gtcgatagcc
    12421 agcgttcacc gaacgcctgc tgcgaaagtg tgttcaacag ggagtactgc tcatcgggta
    12481 gcagtttgtt ctcttccagc aatgagagca tcaacgcgct gtcgcgcagt ggactaccgt
    12541 aatcacccag ccatatccgc tcatcactat tacgcggcgt tttcagcgcc agcacaatcg
    12601 cttcttcacc acgcgttgca tcacccatga ttttcagcgc aacgccaagt tgcagcagcg
    12661 gtaaaccaga agcggcatct gcacgatgct cccagatttc acgcagcgca cccagtggtg
    12721 ctttttgctg acgggccaac accagcgcag cgtaagactg tacggcgaat ttactggctt
    12781 tgagattatc agcgtacggg atcgatatca tgcccggatc ttgtaaatag cgcagcagac
    12841 gctcattacc ccggttaatg gcgtctgtcg gtacgctgta accctgttcg cctgcgcgga
    12901 ccaggaaatc catcacgtaa gccgtcagcc agtactcttc gtcaccgttt ttatcccaca
    12961 gcgcaaagcc gccgttatca cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg
    13021 atgcacggcg tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcat
    13081 tggtataaag tgacgggaac aggccgctgg cggtttgctc gagacagccg tacggatacg
    13141 cttttaactc tttgatataa cgtgcgatgt tcagcggtgg tttgccgctc aacaataact
    13201 gcccttccag cgtaacaggc gagaagtttt gcaatccatc agccgggagc gcccaggttt
    13261 caccaggctg taacgccgta ccgtaattga ccgtttgtgc cgggaatgcc ggacggacgc
    13321 cgattttcca ctgcttatgc tgatcggcaa cggtttcccc cggtaacgct aacccgctaa
    13381 tggtggcctg aatgtcacca tcgccataac ccgtcagagc acgcaccggg ataaacagcg
    13441 tagtacgcac ccctggcgct aattcaacag gtgcggggga attactcacc agttcaagca
    13501 agccactggc ggtcagagca acattaagtt tttgcggttt atcggtaaga ttagtgatat
    13561 ccagcgtcag gcgcgaggta tcgccactcg ccataaagcg cggcatgtta agttcagcaa
    13621 tcaccggtgc ggcaacaatc actttgcttt cgttgcttcc gaagtcatct gccgtccagg
    13681 cttgcgccat gacgcgcaat tcaccgttaa aatcgccaat tggcagtgta accgagcctt
    13741 cgccctgttc attgagcgtt accggcagcg cctgctgcgc gacaatattg acgtgattga
    13801 ccggcggttt accaccacgt ttcagctcat caccatcacc accaaaacgc agagctgcca
    13861 gacgcccctg accttcaata acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt
    13921 tctgaccaaa gaacgcctgc cacggatctg gcgtgacgta atcagtaata ttcagcacac
    13981 cgctatcaac ggcagacacc agcacattca cctgttttgg catctcacca tttttattgc
    14041 tggctttaat tttcacggtt aatggctgat tggggcgcat tttggtgggg gtttccagcg
    14101 ccagatcgag gcgacggttt tcatcaccaa gcggcagatg caacacccca accgcgcgtt
    14161 ttggcgtcgc ggagcgagat ttatcgccag gacgtaccac cagcgtactt aaatacagat
    14221 catgacgatt ccaggtttta tcgaccggaa tcgtcagatc cagcccttga gccggaacat
    14281 caatctcttg ccaccacaac ggcccttcac tggactcgac catcgcataa cctttacccg
    14341 ccgttggcgc ggcaatatgc aacttaatgg tatcgccagg gcgataactg gctttatcca
    14401 gtttcagcgt gacacggtcg ggtcgcactg caccaccccc gtcgctgtta tcctgccagc
    14461 tataaccagc ccagaaacgc acactactga ccgcttcatt cggcgcttta acttccagac
    14521 gataagcgcc ccactctacc gggaaactga ccttaccggt ttcgtccgct ttcagatcca
    14581 gagtttgttc attttcgatc agatcttttt gatcaaactg tgactgccag ccttcatatt
    14641 ccgaccagtt ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca
    14701 cggctttttt cacgccttgc gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt
    14761 catcaacaat gggctgtttc accgtgctgt cggtgcgata atcgtaaacc gatttcgagg
    14821 cgaactgcgg acggatcccc ggcaatgcat cggcaggcca gatagcctgc tcagcacggc
    14881 gcgtcaccgg gcgaccgccc gattccagca aactaccctg gaaaataacc tgtaatggag
    14941 aatgtgtttc cttccactgg ctttctgtag aaacttcgcc gcgcccttta tcatccagcg
    15001 tcaactgaac ttcatccagc gtgcgggaaa gattttcggc agctatatcg ccgaattcaa
    15061 aaccaggtaa ggctgacaca gcttcacgca gtgggcgcag gaaaagttgc ccttgcaaag
    15121 tattaccatt cgcaagtgca ccatacaggt agtaccccac cacggagaat ttcacttcat
    15181 ctttcggcgt tagcggggtt ttctcaccgg tcagattcag cgccatacgc tctggcataa
    15241 aatcttcgac gtggaaatcc cacatccgat actggttatc gcccgtgtta gcgcgaatat
    15301 gccacatacc ggttgccgca ttgctatcga gtggccaggt aaagtggtag aggccattct
    15361 ccggctgact aacgacgctc ctgagtacct gcccgtcggg tttaatcacg tctaacttga
    15421 tgggttgatc gggcaatgct ttaccgtctg catcacgcag caaaccattg aggatgaccg
    15481 tttcacccgg gcgataaaga tcgcgcgggc caaacatgaa aaactgtttg ctatagcctg
    15541 gcgcgccagc aatgttaaat tccgctaagt ccagcgccgg gagttttaaa tcgagcagcg
    15601 ttgtctgacc gtttttacgc gccagcagta atgccgcgtt tttatcattt tccagttgca
    15661 catgcccctg agcgtcactg gttgcctgag tcagagtctg ccctttctca tttaataaag
    15721 agacttcaat tccttgcagg gccgcgccgt tttccagact ttgggtaaag atatccagac
    15781 gattgtgata acggtgagct gaaacgccaa tatcacttaa cgtaaacagc gtcgcgggat
    15841 tactgtaatc gtaacgtcca gcctgattca tcacagccag atacacgccc gcctgctgaa
    15901 gcggtttgat atcgcccagc ggcagcaata atttttcacg ggtgttacgc gcaggattga
    15961 gatcaaaccg tccggtgtag accagatccg ccatctgcag cagtttatct gactgccagt
    16021 tcgccagcga attgcggtat tcccactggc taatgaaagc gggcagtgat tctggtttca
    16081 cacggaagaa gttaacatcg acattgttaa cgttaagcgc cattaccggc agcccttcaa
    16141 cgaccttacc aggaagcaac gaaccacgac tggcaaaacc gacgctgggt tggatatcgc
    16201 gggtagttat agttttttcg tcatctttac taaaggttgc gttattgagc gctttgacct
    16261 ccttgccaat agtgacgatc aaatcacgtt tcggttcgag gtggcgtaaa cgcagctctt
    16321 taagattatc tgacagctcc caggcaccat ccactttgcc gcttttttta tcgacgacat
    16381 gaataacgcg tgagaaatcc tgatccgggt cgagagggat ggagaacgtc agcaccagcg
    16441 tggctgcgcc atcgagttga acttcagaga gatccagcaa tgtcagcgcc ttaccggcac
    16501 tctgttgggc cagtttttgt cgctccggcg cggaaagttt tgctgaactc acgttttctg
    16561 aagaggccgc tttagtaact tcagaaggcg catctttttt caccgctgtt ggcgcgttat
    16621 cgttgttgtc gcaccctgcc agcgccagca ttagcatgca ggcggctacg cgtaactttt
    16681 tcatttttca tccctgccac aatggcccgt tagcaacgtc gaataattat tatgcgtgag
    16741 aatttacgtt atgtaattca gtatcacgcc catacaatcc gaattttagt gagaattgtg
    16801 gctcagacag ccaaatcagc ctcttttgct tgtcgctttt cactaaacat ccgacaattt
    16861 agcctaaccc cggcaaaaat ggagatgcct atgtccacga catggtttgt aggagccgac
    16921 tggctcgccg aacatattga tgacccggaa attcagatta tcgatgcccg catggcgtcg
    16981 cctggacagg aagatcgtaa cgttgctcag gagtatctga atggacatat tcccggcgca
    17041 gtgttttttg atatcgaagc gctttctgat cacacttccc cgcttccgca catgctgccg
    17101 cgcccggaaa cgttcgccgt ggcgatgcgt gaattaggcg ttaaccagga taagcacctg
    17161 attgtctatg acgaaggtaa tcttttctca gccccacgag catggtggat gctgcgcacc
    17221 tttggtgtag agaaagtgtc gattctgggt ggtggacttg caggctggct gcgtgatgat
    17281 ctgctattag aagaaggcgc agtagagctg ccggaaggag agtttaacgc cgcgtttaat
    17341 cctgaagccg tggtgaaagt aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa
    17401 attattgatg cccgcccggc tacacgtttt aacgcagaag ttgatgaacc tcgcccaggt
    17461 ttacgtcgcg gacatattcc cggtgcattg aacgttccgt ggacggaact ggtacgtgaa
    17521 ggcgaactaa aaacgaccga tgaactggat gcgatatttt ttggtcgcgg tgtcagctat
    17581 gacaaaccaa ttatcgtcag ctgcggttct ggtgtaacag cagccgtggt tttgttggca
    17641 cttgcgacgt tggatgtgcc aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc
    17701 gcacgggcag atttaccggt tgagcccgta aaataagctt tttacgggca ataaaaaacc
    17761 gccgaatttg gcggtttttt attgctggtc tggttcgcgg cctttccagc aggttgtatt
    17821 accgtagtaa tgcaagcgcg tctcagcgga cacaatactc gccagtaact ctctttttgt
    17881 caagtaaaag agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc
    17941 gtctcgcaag cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag ggcaagcgcg
    18001 tctcagcgct tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca
    18061 tggaccgcac aatgaagata cggtgctttt gtatcgtact tattgtttct ggtgcgctgt
    18121 taaccgaggt aaataataac cgaagtctct tcggcgacaa tttactggtg gttaacaacc
    18181 ttcagagcag caagtaagcc cgaatgccgc cctttggggc ggcatatttt agattatccg
    18241 gttctgttta aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg
    18301 ttcggtaata aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgatggg
    18361 ttcatcacca ggtaatgtat cggtcgctac acttcccgcc acctgaataa tttcttcgat
    18421 atcaccatcg gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat
    18481 tgaacaaata aaagcacgct tcaccggctt aatggtttta aataaggtgg taagtgaatc
    18541 aatcatttgc gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag
    18601 tacttcctgg ctactcagcg gattcccttc ttcaccaatc aacaaactga tttcacgcgg
    18661 cataaactct ttaccggttg gcagtttggc attaaggaag agcgtttcgc caagggtcat
    18721 ctcaaacaat gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg
    18781 aagtgcttct aacgaggtaa aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg
    18841 ctgtaaatca agcgcgctat cttcaactac tgcctcgccc tgcgccgccg taccaggcac
    18901 ccagacggtg gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc
    18961 tgctttttcc agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca
    19021 ttcaacgttc ggggagcaag tgcctgatgc gccacgctta tcaggcctac aagggagtcg
    19081 caatctattg gattttcgca atttgtaggc cggataaggc gtttacgccg catccggcat
    19141 attagtttac gccgttaaca gattagcaat cgtgcgcaca ccaagtcccg tagcacccgc
    19201 agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc
    19261 ttgctgatag ttctcaacaa agtgcgacag gaagcccgca gccgtacttg cacctgccgg
    19321 atacgccgcg cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg
    19381 gaattccgcc agcggcagac gccagaacgg ttcgttctct tgtgaggcac tcgccagcaa
    19441 acgaccggca agcgcatcgt caaaactgaa caacgcgtga taatcattac ccagcgcagt
    19501 tttcgccgcc ccggtgaggg tcgccgcatc aatgatcagt tccggtttct gcgcactggc
    19561 atcaatcagg ccatcggcca gcaccagacg cccttcggca tcagtgttca tcacttcaac
    19621 ttttttaccg ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctgatcag
    19681 gttatccgca caacagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa
    19741 tgccagcgcc ccggtaaccg ttgccgcacc gcccatatcc gacttcatcg agtccataaa
    19801 cgcggtctgt ttgatgctgt agccgccgga atcaaaagtg atgcctttac caaccaggca
    19861 cgcgtacact ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg
    19921 agaacgttct gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc
    19981 ttcgcctttg gtgatccggt aagtcacacg atcccctgcg acgttgcaga tcagatcaac
    20041 agcacgctgt gccagttgcg atggtcccag ctcttctgcc ggtgcgttga tggtgtcacg
    20101 cacccagtca atgatcatca ggcggttatc cagttcctga cgctgggcat cgtccagctc
    20161 tggccactcc actttacgtg tgcctttcgg ggctttgtaa ccttgccaga atgcccagca
    20221 gcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttaatgccca gaccatcaac
    20281 cttgcgcgcc gcacgctgga tcaaccccag atcgtctgcc ccgttcaaat gcagggtaat
    20341 gccatcatta ttaatgctgt aagttgcttt ttctccccag cgcacgtcgg caggttgggt
    20401 agaaagggta attttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa
    20461 acgggctacc cccaggcagc ccgttcattc actcggcttc gtccagccag accaacaaaa
    20521 tcgcttcgag gattttctcg ttcgatgcct gcgggtcgtc gtcgaaatct tccagagcgc
    20581 aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaatatcg ggatacgcat
    20641 cgtacagtgc ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg
    20701 ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc
    20761 ggtaacgcgc gcctggcagc ttaaacggct ttccggctcc agcccccagg ctttgtccag
    20821 catgtcgtct tcctgctctg agctttccgg cagcgagtca aaaccttcgc gaacgatgca
    20881 gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg
    20941 cagcgcaaca tcgagaatgg tttcaccgct attcgcttcc agaacagcgc catcagggca
    21001 gagatcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc
    21061 ctttcagcgc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt
    21121 tgtctacgtt tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac
    21181 tcaggtgagc ggcagcatcg tcgataacct gacgttctgc ggcgcttaac agcgcggcat
    21241 cagcagccag cgcaccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg
    21301 ccagcattcg ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgagg
    21361 cgatttcgct atcggtcagg ccataagacg gtttgacctg aatagacgcc tcaacgccgg
    21421 tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc
    21481 gaatatgcgc accgccagcc ggtagcgccg gaatacctcg cagcgcaaaa cgcgccagcg
    21541 agcggcagtc ctgcaccagt tcgcgctcac cctgcatcac atggatagac atcgccgtct
    21601 gaccatcttt aaaggtggtg aaatcctggg cgcgggccac cggaatcgtg gtattacgcg
    21661 ggatcacttt ctccaccagg ccgcccatcg tttcgaggcc cagcgacagc gggatcacat
    21721 ccagcaacag catttcgctg tccggcttgt tacccaccag aatatccgcc tgaatcgccg
    21781 cgccaatagc gacgacttta tccgggtcga tagaagtcag cggtggacga ccgaaaaatt
    21841 cgcctactcg ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca
    21901 gcacttcatc agcttctacg cccgcatctt tcagcgcgcg acgacaagcc agtaaggttc
    21961 gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc
    22021 ccgcaacgtt aacggtcaca gagtccgcat cgctcagcgc gattttggcc gcaatggcag
    22081 catcgagcag ttcacgctga acgcggttat cgctacgatc aggaatgccc gcctgctcgc
    22141 gaatgtaatc cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg
    22201 tcgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc
    22261 cgccgaggtc ataaacagcg atcacgcctt cctggccgga atccagcccg taggcaatag
    22321 ccgcagcggt cggttcatta agtaagcgca gaacatgcag ccccgccaga cgcgccgcgt
    22381 ctttggtgcc ctgacgctgg gcatcatcaa agtacgccgg aacggtgata acgacaccat
    22441 ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag
    22501 aaacgcgcac cgggttcagc agccccgccg ccgtttcaat catcggcagg ccattctcgc
    22561 tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc
    22621 ccatcaggcg tttaacagaa ctaatggtgt tggcggtgtc gagcgctgcg ttagtacgcg
    22681 cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagacggc agcaggtgac
    22741 ggccttcatg gtcagctaac gtttcggcct gaccgctgcg cactgttgcc accagcgagt
    22801 tggttgtgcc caggtcaata ccggccgcca ggcgacgctg atgcggcgca gcactcaaac
    22861 caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt
    22921 ttttcttcga gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcaca
    22981 gtatccgccg ccgcgtccca cgtctcgttg tctaactgtt caaccatcaa ctgatggcgg
    23041 gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc
    23101 tgttcgatct cgtccagttc ttcgcgcagt tccagctgtt ccatcaggaa cgcggtgtcg
    23161 cgcacagtat gctgctcgct ggcgagatca aaaccgtgca aagaaagcaa atattccgcg
    23221 cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggtcgcgga ttgctgtacg
    23281 gcggcgagtt gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt
    23341 agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca
    23401 aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat
    23461 caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa
    23521 cgtggaagct ttcgccgcaa ccacactcat ctttgacgtt cgggttggtg aatttaaacc
    23581 cttcgttaag gccttctttg acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt
    23641 tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt
    23701 cgtcaacaaa ttccagtaca taagccatac ctgaacaccc ggaggttctc acgcccagac
    23761 gcaggccaaa ccctttaccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt
    23821 cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta
    23881 tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc
    23941 ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga
    24001 gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg
    24061 taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc
    24121 atcacgtcgc cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgttg
    24181 tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc
    24241 attataaatt ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga
    24301 tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat
    24361 ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag
    24421 aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac
    24481 ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ccgcgaggtc tttcagcgcc
    24541 ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg
    24601 tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacga
    24661 ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag
    24721 gcttcgccca taccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg
    24781 tgaccgccgc catgcatttg tgcttcgata cgtacgcgcg gtttacgacg cacatacagc
    24841 gcaccaatac ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc
    24901 aactggctca gatcgatcgg cagtttgccc acgctctgag ttgcatcaac gtgatagata
    24961 atgccacgag cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg
    25021 ttatttacgt gcatgatgga aacaaggatg gtgtcgtcac gcatcgctgc ttcgagttct
    25081 ttcaggtcga taataccgtt acgctgcggt gccaggtaag tgacttcaaa accttcgcgc
    25141 tccagctgac ggcaggtatc cagtaccgct ttatgttcgg ttttactggt gatgatgtgc
    25201 ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt
    25261 gcaccagagg taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta
    25321 cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg
    25381 ttaccaaagg ttccgtccat cgtcataaac tgcatcattt tctcggcaac acgcgggtcc
    25441 accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg
    25501 tacatcactc aatgcaagga accaggctac cggctggatg tacgaccgtg tttacggagt
    25561 gtttagcact ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc
    25621 gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc
    25681 acttcctggt tattaaccag ttcgcctaaa gtaatgttat tgagaaaacc ggtaagacgg
    25741 tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct
    25801 ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac ctcgccaacg
    25861 gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa
    25921 accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc
    25981 tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca
    26041 agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca
    26101 aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa
    26161 gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta
    26221 accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca
    26281 ccttatccga cctgggattc cgtgcactta caggcctgat aagacgcgtt aagcatcgca
    26341 tcaggcaacc actgttattc ggccttgtta cctttattct gctgctcaat agaagccaga
    26401 atcccgcgca ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc
    26461 aatttattca tcacctgtcc cggatggttt tcacggataa aaccggttgc cagcagcgtt
    26521 tgctccagat gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca
    26581 tgctcgacct gctcgccatt ttcctgagtt gccagccagg ccatgcgcac ttcataggca
    26641 ataacctgaa ccgccatcgc caggttcagc gaactgtatt ccgggttagc cgcaattgcg
    26701 acatgataat ggcatttctg caactcttca ttggtcaagc cgacgcgctc gcgaccaaac
    26761 accagcgcca ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg
    26821 tcgagcatcg gccacggcag cgtgcgggag cgagcgctgg tgcccaccac cagactacaa
    26881 ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc
    26941 cctgctgcca gggcaatcgc ctgggagtca ggtttcacta gtggattaac cagccacaga
    27001 ttggttaatc ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac
    27061 gtctccacca gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt
    27121 cacgcatctt atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt
    27181 ttcccgttct ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg
    27241 cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta
    27301 gaagcgagcc agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg
    27361 gtgattatcg acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc
    27421 ggtgaacttg aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggcact
    27481 accaacttta tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc
    27541 cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc
    27601 ggtcagggcg cacagctgaa cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac
    27661 ggtactattc tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc
    27721 aacatcgtcg gcaaactgtt taacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg
    27781 ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgc
    27841 ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag caggcggcat cgtcagcgac
    27901 ttcaccggtg gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt
    27961 gttaaagcca tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga
    28021 ctcaggcggg tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc
    28081 ctcaccgctt tactttcttt cgtcactctc ccaccatctt tcgccgatta atggatgata
    28141 aagaagtcgc aaggaaaggt tatggcgctg ccagtgaaca aacgcgttct caaaattctg
    28201 tttattctct ttgttgttgc cttctgcgtt tatttagtgc cgcgcgtcgc catcaacttc
    28261 ttctattatc ctgacgataa aatatacggt cccgacccct ggtcggcgga atccgtcgaa
    28321 tttacggcta aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct
    28381 gctgacaacg ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc
    28441 cactggccgc tggtcagttg gttacccgag cgtaatttca acgtttttat gtttgattat
    28501 cgcgggtttg gtaaatcaaa aggcacgccg tccctggccg gattgctgga cgatacgcaa
    28561 agtgccatca atgtggtgcg ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc
    28621 gggcagagta ttggcggggc gaatattctg gatgttattg gtcagggtga tcgtgaaggc
    28681 atacgtgcgg tgatcctcga ctccaccttt gcctcttatg cgaccattgc caaccagatg
    28741 atccccggca gtggctactt acttgatgag agttacagcg gcgaaaatta catcgccagc
    28801 gtcagcccga tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag
    28861 cacagcgaaa agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat
    28921 ggcgaacaca ttgatgcttt ttccgatcgt cacggcgatg tttatcgcga acagatggtg
    28981 gattttatcc ttagcgcatt gaatccgcag aactaaccca tgatcgctag cacgataatc
    29041 attcacaaaa ccaccttaag acatgctaat ccactggtca gaacagttta agatgagaaa
    29101 aattctgtga tgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag
    29161 caaactttag ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct
    29221 tgctccacca tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt
    29281 tcagccgggg ttgaccgcca ccacggcaac tttcagcgag cttaatggtg tggatgatga
    29341 tatcgccagt cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac
    29401 actgacgact ggttatgacg gcagctaccg ggttgaaggt acagtgctta accaacgttt
    29461 gtgtttattt cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagccg
    29521 attcaccccc gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga
    29581 cgataccaat ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga
    29641 aacgcgcgat attcatttcc tgtgtctgta tttgcaatat tgtttgttgc aacatcacgc
    29701 tggaattacg cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt
    29761 tcaggtagcg caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc
    29821 tgatgagcca ctatttatgg cactactttt ttccatgttg cgggttcccg atccactgcg
    29881 ggatgcgcat cagcgggaca gacaactgcg tcagtctatc aaacgtctgg taaaccattt
    29941 tcgtgagctg ggaaatgttc gtttttatga tgaacagggg ttatgcgatc agctttatac
    30001 ccacctcgcc caggcgttaa atcgcagttt gtttgccatc ggtattgata acaccctgcc
    30061 ggaagagttc gcccgcctgt atccacgcct ggtgcgcacc acccgcgcgg cgctggccgg
    30121 atttgaaagt gaatacggcg tccatctttc tgatgaggaa agcggtctgg tcgcggtgat
    30181 ttttggtgcc tggctaatgc aggaaaacga cctgcatgag aaacagatta ttctactgac
    30241 cgggaatgat agcgagcgag aagcgcagat tgagcagcag ctacgcgaac taacgttact
    30301 gccgctcaac attaaacata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgcgg
    30361 cgcggcactg attattgcgc cttataccat gccgttaccg ctcttttcac caccgctgat
    30421 ctatacggac ctgacgttga caacacatca acaggagcag atccgcaaaa tgctcgaatc
    30481 agcatgaggg aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatca
    30541 cccagaacac gccgtggccg agatattgat acaggaaacc ggcgaaaacg gtcatgatgg
    30601 cgatactgcc gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac
    30661 cctggcgagc agcaatatag cgcatggcag ccaggtggca gaccgtgaag gtaccgcaat
    30721 gcagaatttg caccactatc aaccacggca acgccgtcgt tgctcccata atgccccagc
    30781 gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa
    30841 gtttattact cagcgcaaaa ataatgactt ccgccaccac gcccagcgac cacaaatacc
    30901 ccaccgccga ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg
    30961 ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca
    31021 acgccaacca cgcagaccag ccggtgctct cctgctggcg gcttgcccct tgtggctgaa
    31081 tcgtcggacg gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcgcga
    31141 ggatcacccg ataatcaaac atactgacca gtttgcccgt cagcgccgag ccaatgacaa
    31201 acgccaccga gccccacagt ctcactttgc cgtaatcaag cgggaactgc ttttgccacg
    31261 tattcgccag tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca
    31321 tcaccagcat tagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg
    31381 tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg
    31441 cgatgagcaa actcccgagg aaacgggcaa ccagacccgc ccccaataac aggccgatgg
    31501 tttctggcgt taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt
    31561 aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga
    31621 gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa
    31681 taagaatatt cgagcctgct tcctgacggt tatcctgcat atcaggaaat gacaaaaaag
    31741 tgcggcccac aacacaacat gtaaaaaaga acgatgaaac aggtgaaaat cgcaatttat
    31801 tgaactgaca aactcatgtg agacggataa ggcgtgcatg tcgcattcgg caggaaacaa
    31861 taagcacatt gtcagccatc tgcgttatgc cgttatacca gaaacgaata tctcaacgcc
    31921 tggggcgtgg cgaggtacca gagtgtgtat tacgtttaaa tcacatcatc ttgcaaaggg
    31981 attggttatg aactcactac gttattttga ttttggcact gcccgccccg ttttattatt
    32041 aattgcccgt atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg
    32101 ctttgacggt acggtccaat atatggcctc gctgggcgcg ccaatgccga tgctggcagc
    32161 gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac
    32221 ccgtccgctg gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca
    32281 ttactgggat atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt
    32341 cagtatcgct ggcgcattcc tgctgttggc aattaccggg ccgggggcga tttctctcga
    32401 tcgccgttag gaaagatgcc ggatgtggcg tgaacgcctt atccggcatt ttataaatta
    32461 cagcaacgca acccgcaacg acatctctga aattgttgat tcccctggtg caagcgcaat
    32521 gaggtcacca ccttccgggc gatgatggtc atccggcgca tgactcatcg gttccagaca
    32581 gaagaaatca aacgcatatc ctttatcaaa cgcagggtct gaaacaaaga tgaaataaca
    32641 cggtgcaggt ggcgtcgttt ccatgatgat ggcatacccc tcctgcggct gctcgatgcg
    32701 ggccttacca ttccatccgg caaaaccatt gtttacccac tggcgcggca acggcgcgag
    32761 ctggttaaaa tccagctcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc
    32821 ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa
    32881 ataaggatgc cagccggtac caaatggcag cgtctctgcc ccttgattgg tgacagcgag
    32941 cgtcaccgtc agcgtatccg ccgttaaatg aaatgcctgg cttacccgat agtgataaac
    33001 accgctgaga tgttcataca ccagacacaa gctatcatcg ctgcgcgaga cacattgcca
    33061 ctcgcccaac cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag
    33121 ttgatactca cgcccctgcc agacaaaacg gttgccgctc acccggtttg caaatggtac
    33181 cagcggaaaa caagaggcat cggttgccac accgcttttt ttaccaggac gtagcagcgg
    33241 cgtcgtatcg cgccagaagc cttcgattac gccgccctga tcagaaacgt ccagctttag
    33301 cggcccgtga gataaggtat agatggtcat ccgcagactc cttaatccgg gaaattaatc
    33361 acaactttcc cacatttgcc gcttgccatc agtgcataag catctcctgc ctgttccagc
    33421 gagaaacgat gggtaatggc gttacgcggc cacagtttcc agtccgttaa atcgtgggcg
    33481 catttttcca tatggaacaa actggtcacc caggagccaa taatccgccg ttgatggtgc
    33541 atcaggtcag cgctaacctc gaactcgact tttccggttt caccaatgta aaccacgcgt
    33601 ccccagtcag ctgtggattg cagtgccagt aagcgacctg cggcattacc ggaacaatca
    33661 agcgcaacat ccgcgccgcc gtgagtgagt tcggcgatta tctgcggcag accttcggtg
    33721 gtcgctaaat agccgtgatc catcactcct aactgtttcg ccatcgccag acgttccggc
    33781 agcatatcaa cgccgatgat cctttttgca ccacgacctt tcgccagcat catcgccatc
    33841 atgccgaccg gccccagacc gaccaccagc acgttatcgc taccggaaac ttcgccgcgc
    33901 aaaatccctt catacgctgt accaacgccg caactgataa acgcaccatc ttcatagctc
    33961 agcgcgtccg gcagcaggat cagatctttt tcttccgcca gcaggtattc agcatggccg
    34021 ccgtcacgct gccagccgta agccgctttt ccttcgccag tacaagaaat aggaaaaccg
    34081 cgacggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc
    34141 tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg
    34201 ttgataaatc cctggtataa cggtttatcg ggtgccgccg ccgtagcgcg gtgctggtgg
    34261 tagatatagt ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg
    34321 ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggtcgaatt tcctggtaaa
    34381 taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct
    34441 gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactacta cgctgggtca
    34501 gcaagatatt tgccagcacc gccaccacaa tgatgacgcc gcgcaccacc tgctggaaaa
    34561 aggagttaat cccgagcagc accagaccgt taccgattag cgtaatcacc agtacgccaa
    34621 gcagcgtacc gaacaacgag ccgcgaccgc cggaaagcgc cgtaccgccg accacgaccg
    34681 cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgcg
    34741 ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaaa ataagaatgc
    34801 gtacccgacg aacgttaata ccgcacaact gcgctgcagt ggcattaccg ccaacagcaa
    34861 aaaccgagcg cccgaaggcg gttttgcggc taatgaacac aaacagcgca aataacacca
    34921 tcatgatcag cgcggatacc ggcacaccga gaaattgtcc gcccagccag tccagcactt
    34981 cgttttcgtt aattggcact ggcaaggcgt tcgtcataaa cagccccatt ccgcgcaggg
    35041 cgctccataa acccagcgtg gcaacaaaac ttggcacgtt aaacacgccg cgcagcaccc
    35101 cggcgagcgt tcccatcagc gcacctaaca gcaacaccag cagacaagca atcgccagcg
    35161 gaacttcaaa ttgcagcaaa aacgccaggc acactgaaac aaaagccacc atcggcccaa
    35221 cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcgatcccga
    35281 tggtggcggc atcgcgcagc acgttcattt ggttattcaa tgagataaag ccaggcgcat
    35341 tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat
    35401 ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag
    35461 cagacatctt caggttcctc ggtaaaattc atcagtggac agaatggcgg acatcagctc
    35521 atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat
    35581 gcggtcacac accagcggca actcctccac ttcactggag ataaacacca cgctttttcc
    35641 ttcggcagcc agctcgcgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    35701 gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    35761 cactttttgc tgattgccgc cagaaagcgt gccgatgggc gtttcgctac tggcggcctt
    35821 gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac
    35881 accgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca
    35941 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    36001 gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc cctgttcata
    36061 cgtctccaga ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcagaccagc
    36121 aatgccgagc acttcgccac gacgtagcgt aaaactgata tcctccagct tgggcttatg
    36181 gcgtaacgca cggacttcca gcacggcttg atccataatt tcctgagggg caaccggcgc
    36241 aatatcaacg tgatcgcgcc cgagcattag cgacacaata tgatgcgtag aggtgttttc
    36301 gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg
    36361 gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt
    36421 tttcaccgcg ctgatcacca gttcaacttc cgcactggca agcgagctgg tgggttcatc
    36481 aagaatgacc acgcgcggct cgcccttcat cacccgcgca atttccacca gttgcttttg
    36541 cgccgggctt agcgttgaaa caagttgttc agggctaacg tcaacgccca gcgcctgtaa
    36601 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca
    36661 ctgaccgagg cagaggtttt ccgccactgt cagcccttcc accagactta actcctgata
    36721 aaccgcgcgt acccccagtt cagcggcacg gcgagtcagc gtagcttcgt caccttccag
    36781 tcgcgtctcg ccaatccaga tatcaccgct atccggacgt tcgctaccgg taagcattcg
    36841 aatgagggtc gattttcccg caccgttttt gcctaacagc gcacgaactt cgcctttttt
    36901 gagcgtgaag ttaacgttat ccagcgcaac gactccggga taacgcttat ttcctgccac
    36961 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    37021 attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc gttttggtat
    37081 agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    37141 atgtttgcgt aaaaacagca ttgcccattt tcttaccaga aatatccacc accgctttca
    37201 gcacctggtt gttttccagc tcctgagcaa tttcagtggt catatcagaa ccgaaaacag
    37261 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgctccgc
    37321 ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccgacag
    37381 aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc
    37441 gggtttttaa tacttcttca aaccctttac gacgttgcac acaaacttca aaggcttcgc
    37501 aattgatgac ggcaattttc ggctggtcaa ttttgttggc aataaaataa tcggcggcag
    37561 cgttacccag ttttttacca aattccagcg ggtcaccaac cagatatgcc gagacatatt
    37621 tatcgacacc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    37681 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat
    37741 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcgccctgag
    37801 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct
    37861 gacgcactcc ggcgtaatat ccctgggtat caaggtatat tgcgccaata gtcatttctt
    37921 tatcagaagc cctggcaaat agtgcgctac ccaatagtgt agccattaat aataaattac
    37981 gggttcttgt cattttttta ggcatagtgc ctcctgtagg gtttttattt acaacggctt
    38041 attttaatta tttgagcgtg agcggcagca cgtgccgctc aaatattaca gttaataggg
    38101 tttagtgaac gtagctaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc
    38161 aggctggatt aatcgccatc aattcagcgc gagcagattc atagtgcgca acctccccta
    38221 atcccgcggc cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
    38281 cgccatacag cgacaacaag tcaggctgcg agacggcaaa gaaatccgcc tcgatactgg
    38341 ttttcgccat ctcttgtgcc cactgtttca tttcactaaa cagttgctgt gcagtttgtt
    38401 gttcgcccag cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt
    38461 tgtaataact gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgcg
    38521 tcgcttcagt ttcatcgccc tgcgcgttgg cgcatactgc ctgccagaac cagatgtcgt
    38581 tgtcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat
    38641 gaagcagttc gctggcctgc tgcggctctc tgtcatcaag atgctgccag gcgcgtaata
    38701 actggttgag gataaactgg ctggtgacct tcccttcccc accttcccac gggtggaatt
    38761 tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga
    38821 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattatct tccagacgcg
    38881 ccagtcgttt ctccggtgtg acaccgctta acttatccag caaatcccgt tcgaaaagca
    38941 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
    39001 aatcgtgctg cttattccac gcatggatcg ccaacccgcg ccagccgtcg gcaaactccg
    39061 gcgacatctc tacgcaacgc tgccataagg caatggcttt gccgtagcta cgcttgttgt
    39121 agtagaagca ggccagtaaa tgccgggcaa accagcactc ttcgatactc tccagcgccg
    39181 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaaggcatca atagcttttg
    39241 cgaccagttc tccgcgttca gcttttggta gcaaactggc ttgcaggtaa agcggtagcg
    39301 tgcgctgaca gtccagtgcg ttcagcatat ctgccgccag ggcgggcatg ccccagttaa
    39361 tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacaaaga cctcgccact
    39421 gcgcgagggc tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca
    39481 gcggataatc acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
    39541 acaccagtag caggttatgc aggcaaagca cttcctgatt ggttgggcag gcgcgaagac
    39601 tttgttggca aaaatccaga ccagcgtcga agttaccatt acgcgccgcc agtcgtgcca
    39661 gaccataata gccgcccgct ttactgttgc cgctccagac cgcacgccag aaatcctctt
    39721 cggcttgttg atattgcccc tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc
    39781 cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag tgcctgactg gcatatacca
    39841 ccgcttgcgg gaaatctgca cggttatatt ccagcattgc cagcgccaga ttacagcgat
    39901 aatccagcgg atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt
    39961 gatgatattg ctccagatgt tgaccgataa accaggcttc atctgtactg gtaatgtctt
    40021 gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa cgcttgcggc tgatgttcat
    40081 gataactcag tacaatattg ccgtcggcat cggagagctc aatggtcagc ctttcaggat
    40141 tgataccgtg caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga
    40201 gcaacgcgtt gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga
    40261 tggcatacag cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc
    40321 gggaggcatt ttgcaccatg cccagagaat gataaggcag gaaatactgc tcgaaacgct
    40381 tctcctcgta agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca
    40441 tcagttcgat atacgggccg ttattgtcag tcaggctctt atcccacgcc tggccaaatt
    40501 cactgtgtcc ccagctccac tgttttttac ctggcgcaat atggtggttg gcaacgtgta
    40561 gcaaaccacc atcttcatcg tgacaccacg cgccaacaaa atcgtactgt gatttttcag
    40621 ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact ccggcggagt
    40681 agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt
    40741 gatcaaacac cgccgttaca tccggcggga agacgctctg atgcccttcc ccccctttca
    40801 ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcgac
    40861 tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
    40921 cgtgcatcga ctccgtttcg ccgacccaca ccgtctgtgc gccgtcgtca tgggcttcga
    40981 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
    41041 tcccgccgga gatccacggc cccagcagtc ccaccagcgc cggtttaatg acttcattgt
    41101 gataaacaaa atcgcgctgt ttcactttat cccacgcgcg atgcacccga ccgcccagtt
    41161 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca
    41221 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
    41281 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
    41341 tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
    41401 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
    41461 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
    41521 tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
    41581 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcgg tcagtaatat
    41641 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa gtcaaacgcg
    41701 cggacatagt agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgtctgaa
    41761 cgtgacgccc accagcattg agtgtcaggt cgctaatgcc tgtttaagtc cgaaaggcga
    41821 atttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
    41881 atgctggcat cgccaccgta aattgtggcc ggatcgcacc atcaatctgg ctctggcaat
    41941 ccacggtcag gtggatccgg tgaccggcgt gtcgcaaacc atgccgcaag cgccgtgggc
    42001 aacaccgatt gaggtgaagt acctgctgga agagaagctg ggtattcggg taatggtcga
    42061 taatgactgc gtgatgctgg cgctggcgga gaaatggcaa aataattcgc aagtgcgtga
    42121 tttctgcgta atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
    42181 ttatcgcggc agtttgtatg gcagtggaca gattggccac accatcgtta atccggatgg
    42241 cgtcgtctgc gactgcgggc gttatggctg cctggaaacc gtcgcctcgt taagcgcatt
    42301 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttaatcctga
    42361 aaaattgact accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggttcaccag
    42421 ttgggttgac cacagtgcca acgccattgg tttgagtctg tataacttcc tcaacatcct
    42481 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa
    42541 taccattatt cgccagacgg gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
    42601 agcgacacag attggctttg ggcaattaag ccgcgcacaa caggtactgg gaattggcta
    42661 tttgtatgtt gaggcgcagt tacggcagat ttgatggcgc gataacgtag aaaggcttcc
    42721 cgaaggaagc cttttttcaa cagattaacc gtttcgctta tgcgtaaacc gggtaacgtg
    42781 cgcagatgtc gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt
    42841 ccagcacgtc acacatccag ccagccagtt ctttcgcttc ggcttcttta aagccacgac
    42901 gggtaatcgc cggagtacct acacgaatac cggaagtcac aaatgggctc ttcggatcgt
    42961 tcggtacgct gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt
    43021 taccggtcag gtttttatca accagatcaa ccaggaacag gtggttatca gtaccgccgg
    43081 aaaccacttt gtagccgcgt tcgaggaaca cttctaccat cgctttggcg tttttcgcga
    43141 cctgctgctg gtaagttttg aactcaggtt ccatcgcttc tttcagagca accgctttac
    43201 cggcgattac gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt
    43261 ttttgtacag ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca
    43321 gggttttgtg agtggtggta gttactacgt gagcatgagg aaccgggttc gggtagacgc
    43381 cagcagcaac caggcccgca acgtgcgcca tatcaacgaa caggtaagca ccgatgctgt
    43441 cagcgatttc acgcattttc gcccagtcaa ccacgccgga atatgcagag aagccaccga
    43501 taatcatttt cggtttgtgt tctttagctt gtttttccag atcggcgtag tcgatatgac
    43561 cagtagcatc gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag
    43621 aaccgtgagt caggtgaccg ccatgcgcca gattcatacc cagaacggta tcgcctggtt
    43681 ccagcagcgc ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag
    43741 cgtagtcagc gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa
    43801 catactcgca accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca
    43861 gctgagaacc ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt
    43921 cgatgtgctc ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat
    43981 aatcggcaat gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa
    44041 atttttggcc tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg
    44101 tgcgataaca ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg
    44161 ctttggagaa taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa
    44221 tttttcactt catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct
    44281 attttttata agatgcattt gagatacatc aattaagatg caaaaaaaag gaagaccata
    44341 tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg gtggaaacgg
    44401 gaccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca gaactcaaag
    44461 aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg tttaacgctg
    44521 ttgccgccta cgccagcaat attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa
    44581 tcgcgcagaa gcacaccagt ttccagatca aaccggaaca gtacaacatc gtcggtgaac
    44641 acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg gacgcgtggg
    44701 gtaaagccta tggcgtactg gctaatgtat ttatcaatcg cgaggcggaa atctataacg
    44761 aaaacgccag caaagccggt ggctgggaag gtactcgcga tttccgcatt gtggctaaaa
    44821 caccacgcag cgcacttatc accagcttcg aactggagcc ggtcgacggt ggcgcagtgg
    44881 cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt ttcccgcatc
    44941 aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat cgtattgcgg
    45001 tgaaacgcga agagggtggg caggtatcca actggctgca caatcacgcc aatgttggcg
    45061 atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca gatgacacac
    45121 cagtgacgtt aatctctgcc ggtgtcggtc aaacgccaat gctggcaatg ctcgacacgc
    45181 tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa aatggcgatg
    45241 ttcacgcctt tgccgatgaa gttaaggaac tgggtcaatc actgccacgc tttaccgcac
    45301 acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt gatagcgaag
    45361 gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg cagttctatc
    45421 tctgcggccc ggttggcttc atgcagtttg ccgcgaaaca gttagtggat ttgggcgtga
    45481 aacaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtga tttgatgttg
    45541 ccggatggac acatccggca acccttgacg cgttttaaat tgccgcgtcg tcctcttcac
    45601 cggtacggat gcgaatgacc cgtgccacgt cgaagacgaa gattttacca tcaccgattt
    45661 tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg tcgtccggta
    45721 cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgca ccacggtaca
    45781 gctcggtatg gcctttctgg cggccaaaac ctttcacctc agtgaccgtc atgccggtaa
    45841 taccgacttc agccagtgct tcgcggacat cgtccagctt gaagggttta ataatcgcat
    45901 caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc taaacgtaac
    45961 acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtttcgg gaaagcagtt
    46021 tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg
    46081 tgatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc tcaacaaagg
    46141 ttggcagcgc cgtattttca ccctccagcg cctgctccac cagcgcatca ctaattaccg
    46201 gagatgaggt cagcgccaca cactgttcaa tcacgttgac caactggcgc acgttgcccg
    46261 gccagcttgc agtcatcagg cgtttcattg catcggtgga gaacgcgcgg acaaacggtt
    46321 tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg tcttctgtgc
    46381 gctccgccag tgccggaatt ttcaggctga caacattgag gcggtaataa agatcttcac
    46441 ggaattcccc acgcgccatc gcttttggca ggtcgcggtg agtggcggaa ataatccgca
    46501 cgtcaatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc tgcaacacgc
    46561 gcagcagttt gacctgcaac ggtgcaggca tatcgccaat ctcatcaaga aatagcgtac
    46621 cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta aacgcgccac
    46681 gtgcatgacc aaacagctcc gactccagca attgctcggg taatgcgcca cagttaatag
    46741 caataaatgg cttgctgttg cgcgggctgg cgttgtggat agcctgggcg aaaatctctt
    46801 tcccggtgcc gctctggccg ttaatcaaaa cgctaacgtc tgattgtgcc accagccgcg
    46861 cctgttccag caagcgcagc atcagcgggc tgcgggtgac aatcgcctcg cgccaacgtt
    46921 cgtcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat agcgcatctt
    46981 tatcgacagg cttggtgaga aaactgaaca cgccctgctg tgttgcagca acggcatcag
    47041 gaatagaacc atgcgcggta agaataatta ctggcattcc cggctgcact ttctggattt
    47101 cagcaaacag ctgcataccg tccatttcat ccatccgcag gtcgctgatg actaaatcta
    47161 ctttttcgcg attcagtacc cgcaatcctt cagcaccact ttccgccgtg accacactgt
    47221 agccttcgct ggtcagacgc aggccaagca gtttcagcaa tcccggatcg tcatcgacca
    47281 gtaataaatg cgcaggttta tggctcatca ggagtgacct catcggtgga tggcgcgggc
    47341 ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg ggtcgagagc
    47401 tggcgttcaa tatcggtcag gttttccagc ttgcgagtag tgagttccag ttgctgttgt
    47461 agaacgtgat gttgctggcg caaagtatcc agctcgctgt cgctggactg ctggagtttg
    47521 ctgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc gcgccaaagt
    47581 tgatacagtg ggcgaacctg tgccgggatc tcggtactta gcgcatcaat gcgcgcgacc
    47641 agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag
    47701 gtattttgcc agttgccgtc gtcatattgg cgggcttgct gacgcgactg cgcaggcatt
    47761 aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc ggttgattta
    47821 ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag ttgataaacc
    47881 ggaatttttt cttctgctgg cgtatcaatg gctggcctat tgtgactctg cacgcaaccc
    47941 agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa
    48001 atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg ttgcaggcag
    48061 tttggacatg gacagcgcgg agaaaccgga gcgtacatgt cgtacgtgaa gatttcgagc
    48121 actgcccggg gccgaactga caaataaaat agcctgatgg gatgggctgt tagcattatt
    48181 tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga
    48241 ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt aatcccagac
    48301 cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt tcgaagatca
    48361 tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata taaacccgcg
    48421 caccatgtaa actgctgcga aggcaaatgt taccggattc agccccgtag tgcaccgcat
    48481 tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg caagctgttg
    48541 ctttgagatc aacgtcggta tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa
    48601 ccaatgtctc caccagcggt gctaactcaa cattctccag ttccaccgca ctgtccgcct
    48661 gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcgg ctgctgctat
    48721 caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca
    48781 gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga gataaatgtc
    48841 ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac caaagaatac
    48901 gttgcccaac cgagcgtaac tcgctcggtc cactgaacga gacgctattg cccagagaac
    48961 gcccttcccc cagccggttg atcatgcgct cgatattttt caccggaccg ataatcatcc
    49021 tcgtgaaaag cagtaccatc accagactca ccagaaatag caccagcgat tgccaaccaa
    49081 aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag aacaccactg
    49141 tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttccaga cgcgcggcgg
    49201 cagcagcatc gggaccgctg ttgttacact gaagttgagc cagattgttc aaatcctgac
    49261 gtaatgcctg gtagagttta tcgtccggca gcacgcctgc gtgggcatcg agcatttcgc
    49321 tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc agcacgcagt
    49381 actgacggta gctgcgctcc atctccagtg ccgcgttggt catcgcttca ctgcgccggg
    49441 catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagtgca ttcaggcttt
    49501 gccaggcttg ccatgccagc accaacaggg gcagcagaat cagcaaaaat gccagcatta
    49561 ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggcgtta ctctcgtcag
    49621 acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc gggaattacc
    49681 cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg gttgatatag
    49741 ctaagctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc gaattttatg
    49801 tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta ctattatgca
    49861 gtaactgtgc caataaagaa aatagtttgg taacatactg attatacgct gctttgaggg
    49921 gtttatggct tctccgctat ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa
    49981 cttaagataa acttattaga taatattaaa atcaatgagt taagtgtcac caaaaagcga
    50041 cacggcaaac cacccattgt cgtgatttaa agacacaaaa aagctcccga agttgggagc
    50101 tcataatagc ggttggtgct taatgccgga tgcggcgtga atgcctcatc cgggctgcaa
    50161 taccaatggg ttgacgactt accccaactg cttacgtgca ttgcggaaaa tgcgcatcca
    50221 tgggccatcc tcgccccagt tttccggatg ccaggagttg ctgacagtac ggaaaactcg
    50281 ttccgggtgc ggcatcataa tggtgactcg accactttca gtcgtgaccg ccgtaatacc
    50341 gttcggtgaa ccgttcgggt tagccgggta ggtttcagtg actttgccga agttatcgac
    50401 atagcgcagt gccaccagcc ctttgctttc cagtgccgcc agatgcgccg catcacgcac
    50461 ttccacgcgc ccttcaccat gagacacagc aatcggcatc tgcgagccca ccatcccctg
    50521 caacagcagc gacgggcttt gggttacttc taccagactg aaacgcgctt caaagcgatc
    50581 ggaggtgtta cgcacaaaac gtggccacaa ctcgctaccc gggatcagtt cacgcagatt
    50641 agacatcatc tggcaaccgt tacatacccc cagcgccagc gtttgcggac gatggaagaa
    50701 ggttgcaaac tcatcgcgta cacggtcatt gaacaggata gacttcgccc aaccttcacc
    50761 agcgcccagc acatcaccgt aggagaaacc accacacgcg accagggcgt ggaaaccctc
    50821 caggcccgtg cgtccagcca gcagatcgct catatgcacg tcgatagcat cgaagcctgc
    50881 acggtggaaa gctgccgcca tttcaacatg cgagttcacg ccctgctcac gcagcacggc
    50941 aactttcggg cgcacgccag tggcaataaa cggtgctgcc acatcttcgt tgatatcaaa
    51001 cgacagtttc acattcaggc ccggatcggc gtcgtttgat ttcgcctggt gttcctgatc
    51061 ggcacactcc gggttgtcac gcaggcgctg catctgccag gtagtttctg cccaccagac
    51121 acgcagcgtg gtgcggcttt cgctaaatac agtctgcccg ttggcagtaa tcacaaaacg
    51181 gtcaccggaa accgcctgcc caacataatg gacgcaatca gcaagcccat gctgtgccag
    51241 taccgcttca acggcttcac gatcagcagc tcgaacctga atcaccgcac ccagttcttc
    51301 gttaaacaac gccgccaggc gatcgtcacc cagagaggcg atatccgcgt caatgccaca
    51361 atgaccagca aaggccattt ccgccagcgt taccagcagg ccgccatcag agcggtcgtg
    51421 atacgccagc agcttacgct gtgcaaccag cgcctgaatc gcgtcataga agcctttcag
    51481 ttgggcaaca tcgcgtacat ctgccggttt gtcgccaagc tgacgataaa cctgcgccag
    51541 tgccgttgcg cccagcgcgt tattgccttt gcccaaatca atcagcaaca gtgcgttatc
    51601 ttcggtagaa agctgcggcg taatggtgtg acgtacatct tccacgcggg caaatgcgga
    51661 aatcaccagc gacaacggcg acgtcatttc gcgctcttcg ttaccttcct gccagcgggt
    51721 tttcatcgac atagagtctt tacccaccgg tatcgtcagg cccagcgccg gacaaagttc
    51781 ttcgcccaca gctttaacgg cttcatacag gcccgcatct tcaccagggt ggcctgccgc
    51841 cgccatccag ttggcggaaa gtttaatgcg tttgatatcg ccaatttgtg ttgcggcgat
    51901 gttggttagc gcttcaccga ctgccagacg ggcagaagcg gcgaaatcca gcagcgcaac
    51961 tggcgcacgt tcgccaattg ccatcgcttc gccatagtag ctgtcgaggc tggcggtagt
    52021 gaccgcgcag ttagcgaccg gcacctgcca cggccccacc atctgatcgc gcgccaccat
    52081 gccggttacg ctgcggtcac cgatggtcac caggaaagtt ttctccgcca cggtcggcag
    52141 atgcagcacg cgtttcaccg cgtcagcgat ggtgatccct tcacgggcca gcgcgtcgcc
    52201 tttcgctttc agcgtttgca catcgcgggt catcttcggc gttttgccaa gcagtacgtc
    52261 cagcggtaga tcaatcggct gattatcaaa atgacgatcg tgcagagaaa gatgcagttc
    52321 ttcggtcgct tcaccaatca ccgcgtacgg tgcgcgttca cgcttacaca gttcgtcaaa
    52381 cagcggtaac tgatcggcag caactgccag cacgtagcgt tcctgggatt cgttacacca
    52441 gatttccagc gggctcatgc ctggctcgtc gctcagaata tcgcgcagtt cgaatttacc
    52501 gccgcgcccg ccgtcgctca ccagttccgg catggcgtta gaaagaccgc ctgcgccaac
    52561 gtcgtggata aacaggattg ggttggcatc accaagctgc cagcaacggt cgatcacttc
    52621 ctggcagcga cgctccatct ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc
    52681 atcagactga ccagatgcca tggaagacgc cgcgccgccg ccaagaccga tgttcattgc
    52741 cgggccgccg agaacgacca gcttcgcacc aacgttgatc tcgtcttttt gtacgtgatc
    52801 ggcgcgaatg ttgccgatcc cacccgccag catgatcggt ttgtgataac cgcgcagctc
    52861 ttcgccattg tggctgttca ctttttcttc ataagtacgg aagtagccgt tcagtgccgg
    52921 gcgaccaaat tcgttgttaa acgccgcgcc gcccagcggg ccttcggtca tgatgtccag
    52981 cgcggtgaca atgcgctcag gcttaccgaa atcttcttcc cacggctgtt caaagcccgg
    53041 aatacgcaag ttggatacgg agaaaccaac cagaccggct ttcggctttg cgccgcgccc
    53101 ggtggcacct tcatcgcgga tttcaccgcc agaaccggtc gccgcacccg gccacggaga
    53161 aatcgccgtc gggtggttgt gggtttcaac tttcatcaga atatgcgccg gttcctgatg
    53221 gaaatcgtag cggcccgttt cgtggtcagc aaagtagcga cccacttcag aaccttccat
    53281 tacagcggcg ttatctttat aagcagagag aacgtggtct ggcgtggttt cgaaggtgtt
    53341 tttgatcatc ttgaacagcg atttcggctg ctgttcaccg tcgataatcc agtcggcgtt
    53401 aaaaattttg tgacggcagt gctcggagtt cgcctgcgcg aacatataca gctcgatgtc
    53461 gttcgggtta cgacccagct ttgtgaacgc atcctgcaga tagtcaattt catcttccgc
    53521 cagtgccagg ccaagacgca ggttagcgtc gattagcgcc tgacggccct gccccagcaa
    53581 atctacgctg gtgaccggag tcggttgatg gtgtgcaaac aactgctctg catcatctaa
    53641 agcaaaaaag accgtttcca tcatgcggtc gtgcagttca gcggtcacct gctgccattg
    53701 ttcattggtc agcgtaccgg cttctatata gtaagccatg ccgcgctcaa ggcggtttac
    53761 ctgttgtaga ccgcaattat gggcgatatc ggtcgctttc gaagaccagg gagagatggt
    53821 gccaggacgc ggggtcacca gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg
    53881 gccatatttc agcaggcgtt caagttgtgc gtgctcatca tcgtttaacg gcgcattgag
    53941 gtcagcaaaa tggacatact cggcgtaaat agtgtgaacc gggagcctgg cagcctgaaa
    54001 acgtgccagc agtttgttga ttcggaatgc cgacaatgca ggcgaaccac gcagaatttc
    54061 catcataagt ctctcgtctt ctaagctttc ggtgtaccca agggggggaa acgggcgtca
    54121 ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga
    54181 attagcaacg cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa
    54241 aatgccgctc atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc
    54301 gcacgacaca gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca
    54361 ttctggcact gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg
    54421 acaaccgtat cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc
    54481 ccctgactta taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac
    54541 agtttgccga ttacctcggt gtaaaactga aagtgaccgt gcggcagaat atcagccagc
    54601 tgtttgacga cctcgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca
    54661 gtgagcgggt aaaaaattat cagcctggtc ctacctatta ttccgtgtca caacaactgg
    54721 tttataaagt gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca
    54781 ccgttgcacc gggtcacgtg gtggttaacg atctccagac cctgaaagac acaaaattcc
    54841 cggaattaag ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca
    54901 tcgaaggaaa actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg
    54961 ttcacccgga actcgccgta gcgctcgata tcaccgatga acaaccggtg acctggttta
    55021 gcccgttaga tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga
    55081 atgaagacgg tacactggca cgcattgaag agaaatacct ggggcatggc gatgattttg
    55141 attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc
    55201 ccctgtttga gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc
    55261 aggaatcgca ctgggatgcg caggccactt caccgacggg tgtgcgcggc atgatgatgt
    55321 taaccaaaaa caccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca
    55381 tcagcggcgg cgtgcgttat ttacaggata tgatgagtaa agtgccggaa agtgtgccgg
    55441 agaacgaacg gatctggttt gccctcgccg cgtacaatat gggctatgcg catatgctgg
    55501 atgcccgcgc cctgacggca aaaaccaaag gtaatcctga cagttgggct gacgtaaaac
    55561 agcgtctgcc tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc
    55621 gtgggcatga agcctacgct tatgtcgaaa atattcgtaa ataccagatt agcctggtgg
    55681 gttatctgca ggagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt
    55741 atccggcggt atcgcctacg gagctgggca aagagaaatt tccttttctc tcgtttcttt
    55801 cccagtcgtc atcaaactat ttgacccact ctccctctct gctgttttcc agaaaaggga
    55861 gtgaagagaa acaaaattaa tctgtcgagg attgcgcttt tttctgcgct ttaatttcct
    55921 ggcggcgcat gcgaaagaag tcactgagca acgccgcaca ctcatccgcc agtattcctt
    55981 ccgtaatttc cacccggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg
    56041 cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca
    56101 ttgctccggc acacattaca cagggttcaa gcgtgacata caacgtggcg tcgatcagac
    56161 gataattttg catcaccaga ccgccctgcc gtagggccat gatttctgca tgtgcggtgg
    56221 gatcatgtcg accaatcgga cggttccagc cttcgccaat aacacgattg ttatgcacta
    56281 ataccgcgcc gaccggtact tcccgctcat cccaggcacg cttcgccagc gtcattgcat
    56341 gacgcatcca gtattcgtag ctaaattcga cttcagacaa aaagaaaact ccggttataa
    56401 aagcgcggcg cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac
    56461 cgcgcggggt tacccgccag cgatgctggc aaaagtaaag cagcgggttg tcctggttac
    56521 tgtcgctata gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgcgcga
    56581 ccttctcatg cccgaggcaa cgcatcgtca atacccaacc accatagcca cgctgaatct
    56641 gactggcgat aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca
    56701 gcggctgcgg agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg
    56761 ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc acgaaaccag cgtacgaaat
    56821 cggcctgcaa cgcctgcaaa cgcgcttcat tgtgaccaaa agtgcatccc cacagaagca
    56881 gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct atgatcggca
    56941 acaacggcag aacaagtaac gcattcagcg gttggcgacg cagtaaataa cgcaaaaaac
    57001 tgccgaacat atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct
    57061 cgtgagttgc caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac
    57121 atagcctaac agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg
    57181 gaatttttaa ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc
    57241 atattgcctg ttgtcgccat tatttcatcg cgaggaaaga tatgaacggc ttacttcgta
    57301 tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc
    57361 tacaacctga tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca
    57421 gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcactaa
    57481 agttggcgtt gagtgaagcg ctggcaagcc agccggaatc tccctccgtg cccattcata
    57541 accaaatccg cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata
    57601 ctgccgctat gtacgcaacg ttaaacgtta atagtgaaga gaaactgcat gaatgcgtgg
    57661 caatgttgcg ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg
    57721 cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata
    57781 tgcatgcgct actcgctacc gtacaggcgt cgtcccctga cgatctgtta ttagccattt
    57841 cctacaccgg tgtacggcgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg
    57901 gaaaagtgct ggcgattacc ggctttactc ccaatgccct gcaacagcgt gcttctcatt
    57961 gcctgtatac cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg
    58021 cgcagggaat gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg
    58081 caccagaacg tattcgtcat agcgaagcgc tggtgaaaaa actggtctga gtaaagaatg
    58141 cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc
    58201 actaaaaaat gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttcg
    58261 atgggtgaac atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac
    58321 gagacaccaa cctgccagaa ggtgtgtccg atccccaata ctattgtgaa agatccgcaa
    58381 catgtcgaga cggaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa
    58441 atttaa
//
LOCUS       DAPQZD010000010        57002 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPQZD010000010 DAPQZD010000000
VERSION     DAPQZD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.010"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            145..1209
                     /gene="ulaG"
                     /locus_tag="RO454_000624"
     CDS             145..1209
                     /gene="ulaG"
                     /locus_tag="RO454_000624"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDY0932506.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1317..2072
                     /gene="ulaR"
                     /locus_tag="RO454_000625"
     CDS             1317..2072
                     /gene="ulaR"
                     /locus_tag="RO454_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDY0932507.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2069..2818)
                     /gene="yjfP"
                     /locus_tag="RO454_000626"
     CDS             complement(2069..2818)
                     /gene="yjfP"
                     /locus_tag="RO454_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDY0932508.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3000..3329
                     /gene="bsmA"
                     /locus_tag="RO454_000627"
     CDS             3000..3329
                     /gene="bsmA"
                     /locus_tag="RO454_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDY0932509.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3478..3753
                     /gene="yjfN"
                     /locus_tag="RO454_000628"
     CDS             3478..3753
                     /gene="yjfN"
                     /locus_tag="RO454_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDY0932510.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3870..5495)
                     /locus_tag="RO454_000629"
     CDS             complement(3870..5495)
                     /locus_tag="RO454_000629"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDY0932511.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5579..6742)
                     /locus_tag="RO454_000630"
     CDS             complement(5579..6742)
                     /locus_tag="RO454_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDY0932512.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6745..7383)
                     /locus_tag="RO454_000631"
     CDS             complement(6745..7383)
                     /locus_tag="RO454_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDY0932513.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7393..7791)
                     /locus_tag="RO454_000632"
     CDS             complement(7393..7791)
                     /locus_tag="RO454_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDY0932514.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7809..8468)
                     /locus_tag="RO454_000633"
     CDS             complement(7809..8468)
                     /locus_tag="RO454_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDY0932515.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(8518..9216)
                     /locus_tag="RO454_000634"
     CDS             complement(8518..9216)
                     /locus_tag="RO454_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDY0932516.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9235..9636)
                     /locus_tag="RO454_000635"
     CDS             complement(9235..9636)
                     /locus_tag="RO454_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDY0932517.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNNESALIIKLNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9763..10494)
                     /gene="rlmB"
                     /locus_tag="RO454_000636"
     CDS             complement(9763..10494)
                     /gene="rlmB"
                     /locus_tag="RO454_000636"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDY0932518.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10585..13026)
                     /gene="rnr"
                     /locus_tag="RO454_000637"
     CDS             complement(10585..13026)
                     /gene="rnr"
                     /locus_tag="RO454_000637"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDY0932519.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13065..13490)
                     /gene="nsrR"
                     /locus_tag="RO454_000638"
     CDS             complement(13065..13490)
                     /gene="nsrR"
                     /locus_tag="RO454_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDY0932520.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13695..14993)
                     /gene="purA"
                     /locus_tag="RO454_000639"
     CDS             complement(13695..14993)
                     /gene="purA"
                     /locus_tag="RO454_000639"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDY0932521.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15097..15294)
                     /locus_tag="RO454_000640"
     CDS             complement(15097..15294)
                     /locus_tag="RO454_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDY0932522.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15376..16380)
                     /gene="hflC"
                     /locus_tag="RO454_000641"
     CDS             complement(15376..16380)
                     /gene="hflC"
                     /locus_tag="RO454_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDY0932523.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16383..17642)
                     /gene="hflK"
                     /locus_tag="RO454_000642"
     CDS             complement(16383..17642)
                     /gene="hflK"
                     /locus_tag="RO454_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDY0932524.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17728..19008)
                     /gene="hflX"
                     /locus_tag="RO454_000643"
     CDS             complement(17728..19008)
                     /gene="hflX"
                     /locus_tag="RO454_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDY0932525.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19084..19392)
                     /gene="hfq"
                     /locus_tag="RO454_000644"
     CDS             complement(19084..19392)
                     /gene="hfq"
                     /locus_tag="RO454_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDY0932526.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19478..20428)
                     /gene="miaA"
                     /locus_tag="RO454_000645"
     CDS             complement(19478..20428)
                     /gene="miaA"
                     /locus_tag="RO454_000645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDY0932527.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20421..22268)
                     /gene="mutL"
                     /locus_tag="RO454_000646"
     CDS             complement(20421..22268)
                     /gene="mutL"
                     /locus_tag="RO454_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDY0932528.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22278..23609)
                     /gene="amiB"
                     /locus_tag="RO454_000647"
     CDS             complement(22278..23609)
                     /gene="amiB"
                     /locus_tag="RO454_000647"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDY0932529.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23628..24089)
                     /gene="tsaE"
                     /locus_tag="RO454_000648"
     CDS             complement(23628..24089)
                     /gene="tsaE"
                     /locus_tag="RO454_000648"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDY0932530.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24061..25608)
                     /gene="nnr"
                     /locus_tag="RO454_000649"
     CDS             complement(24061..25608)
                     /gene="nnr"
                     /locus_tag="RO454_000649"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDY0932531.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25607..26746
                     /gene="queG"
                     /locus_tag="RO454_000650"
     CDS             25607..26746
                     /gene="queG"
                     /locus_tag="RO454_000650"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDY0932532.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26729..26782)
                     /locus_tag="RO454_000651"
     CDS             complement(26729..26782)
                     /locus_tag="RO454_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932533.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(27016..27091)
                     /locus_tag="RO454_000652"
     tRNA            complement(27016..27091)
                     /locus_tag="RO454_000652"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27056..27058),aa:Gly,seq:gcc)
     gene            complement(27127..27202)
                     /locus_tag="RO454_000653"
     tRNA            complement(27127..27202)
                     /locus_tag="RO454_000653"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27167..27169),aa:Gly,seq:gcc)
     gene            complement(27239..27314)
                     /locus_tag="RO454_000654"
     tRNA            complement(27239..27314)
                     /locus_tag="RO454_000654"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27279..27281),aa:Gly,seq:gcc)
     gene            complement(27525..28070)
                     /gene="orn"
                     /locus_tag="RO454_000655"
     CDS             complement(27525..28070)
                     /gene="orn"
                     /locus_tag="RO454_000655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDY0932534.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            28165..29217
                     /gene="rsgA"
                     /locus_tag="RO454_000656"
     CDS             28165..29217
                     /gene="rsgA"
                     /locus_tag="RO454_000656"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDY0932535.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            29314..30282
                     /gene="psd"
                     /locus_tag="RO454_000657"
     CDS             29314..30282
                     /gene="psd"
                     /locus_tag="RO454_000657"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDY0932536.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            30304..33627
                     /gene="mscM"
                     /locus_tag="RO454_000658"
     CDS             30304..33627
                     /gene="mscM"
                     /locus_tag="RO454_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDY0932537.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33658..33972)
                     /locus_tag="RO454_000659"
     CDS             complement(33658..33972)
                     /locus_tag="RO454_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDY0932538.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(34070..35572)
                     /gene="yjeM"
                     /locus_tag="RO454_000660"
     CDS             complement(34070..35572)
                     /gene="yjeM"
                     /locus_tag="RO454_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDY0932539.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(35791..36768)
                     /gene="epmA"
                     /locus_tag="RO454_000661"
     CDS             complement(35791..36768)
                     /gene="epmA"
                     /locus_tag="RO454_000661"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDY0932540.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            37093..38901
                     /gene="frdA"
                     /locus_tag="RO454_000662"
     CDS             37093..38901
                     /gene="frdA"
                     /locus_tag="RO454_000662"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDY0932541.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            38894..39628
                     /gene="frdB"
                     /locus_tag="RO454_000663"
     CDS             38894..39628
                     /gene="frdB"
                     /locus_tag="RO454_000663"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDY0932542.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            39639..40034
                     /gene="frdC"
                     /locus_tag="RO454_000664"
     CDS             39639..40034
                     /gene="frdC"
                     /locus_tag="RO454_000664"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDY0932543.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            40045..40404
                     /gene="frdD"
                     /locus_tag="RO454_000665"
     CDS             40045..40404
                     /gene="frdD"
                     /locus_tag="RO454_000665"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDY0932544.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            40467..41600
                     /locus_tag="RO454_000666"
     CDS             40467..41600
                     /locus_tag="RO454_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDY0932545.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            41689..42222
                     /gene="blc"
                     /locus_tag="RO454_000667"
     CDS             41689..42222
                     /gene="blc"
                     /locus_tag="RO454_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDY0932546.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(42219..42536)
                     /gene="sugE"
                     /locus_tag="RO454_000668"
     CDS             complement(42219..42536)
                     /gene="sugE"
                     /locus_tag="RO454_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDY0932547.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(42712..42858)
                     /gene="ecnB"
                     /locus_tag="RO454_000669"
     CDS             complement(42712..42858)
                     /gene="ecnB"
                     /locus_tag="RO454_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDY0932548.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(42969..43094)
                     /gene="ecnA"
                     /locus_tag="RO454_000670"
     CDS             complement(42969..43094)
                     /gene="ecnA"
                     /locus_tag="RO454_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDY0932549.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(43146..43712)
                     /gene="efp"
                     /locus_tag="RO454_000671"
     CDS             complement(43146..43712)
                     /gene="efp"
                     /locus_tag="RO454_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDY0932550.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            43754..44782
                     /gene="epmB"
                     /locus_tag="RO454_000672"
     CDS             43754..44782
                     /gene="epmB"
                     /locus_tag="RO454_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDY0932551.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            45017..45886
                     /locus_tag="RO454_000673"
     CDS             45017..45886
                     /locus_tag="RO454_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDY0932552.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(45936..46289)
                     /locus_tag="RO454_000674"
     CDS             complement(45936..46289)
                     /locus_tag="RO454_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDY0932553.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(46427..48073)
                     /gene="groL"
                     /locus_tag="RO454_000675"
     CDS             complement(46427..48073)
                     /gene="groL"
                     /locus_tag="RO454_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDY0932554.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(48117..48410)
                     /locus_tag="RO454_000676"
     CDS             complement(48117..48410)
                     /locus_tag="RO454_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDY0932555.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            48686..49942
                     /gene="yjeH"
                     /locus_tag="RO454_000677"
     CDS             48686..49942
                     /gene="yjeH"
                     /locus_tag="RO454_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDY0932556.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(49958..50434)
                     /gene="fxsA"
                     /locus_tag="RO454_000678"
     CDS             complement(49958..50434)
                     /gene="fxsA"
                     /locus_tag="RO454_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDY0932557.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            50771..52207
                     /gene="aspA"
                     /locus_tag="RO454_000679"
     CDS             50771..52207
                     /gene="aspA"
                     /locus_tag="RO454_000679"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDY0932558.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            52325..53626
                     /gene="dcuA"
                     /locus_tag="RO454_000680"
     CDS             52325..53626
                     /gene="dcuA"
                     /locus_tag="RO454_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDY0932559.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            53742..54080
                     /gene="cutA"
                     /locus_tag="RO454_000681"
     CDS             53742..54080
                     /gene="cutA"
                     /locus_tag="RO454_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDY0932560.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            54056..55753
                     /gene="dsbD"
                     /locus_tag="RO454_000682"
     CDS             54056..55753
                     /gene="dsbD"
                     /locus_tag="RO454_000682"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDY0932561.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            55790..56365
                     /locus_tag="RO454_000683"
     CDS             55790..56365
                     /locus_tag="RO454_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0932562.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            56472..56547
                     /locus_tag="RO454_000684"
     tRNA            56472..56547
                     /locus_tag="RO454_000684"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56505..56507,aa:Phe,seq:gaa)
BASE COUNT        13888 a        15302 c        14162 g        13650 t
ORIGIN      
        1 aatctttcta cactcaaaat ccacaataac tctcctgcta aaaagtcaaa aaatggtcat
       61 tgccttcctg aaaaaatagt gtcaggatta atcaaaacca atcacatatt gattcgaatt
      121 gaactatcac gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg tgagtcctgg
      181 atcctgagca ctttcccgga gtggggtagc tggctgaatg aagaaattga gcaagagcag
      241 gtcgcaccag gcactttcgc aatgtggtgg ctcggctgta ccggaatctg gttgaaatca
      301 gaaggcggtg ccaacgtttg tgttgatttt tggtgcggta ctggcaaaca aagccacggt
      361 aacccgttaa tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag
      421 ccaaacctgc gcaccacccc gtttgttctt gatccgtttg cgattcgcca gatcgacgcg
      481 gtactggcga ctcacgatca caacgatcat atcgacgtta acgtcgctgc tgccgtgatg
      541 cagaactgtg ctgatgacgt accgttcatt ggaccgaaaa cctgcgttga tttgtggatt
      601 ggctggggcg taccgaaaga gcgttgcatc gtcgttaaac cgggcgacgt agtgaaggta
      661 aaagacattg aaattcatgc acttgatgct ttcgaccgca cggcactgat cacgctgcca
      721 gccgatcaaa aagcggctgg cgtactgccg gacggcatgg acgatcgcgc ggtaaactac
      781 ctgttcaaaa cgccgggcgg ttccctgtat cacagcggcg attcccacta ctccaactat
      841 tatgcaaaac atggtaacga gcatcagatc gacgtggctt taggatcgta cggagagaac
      901 ccacgtggta tcaccgacaa aatgaccagt gctgatatcc tgcgtatggg cgaagcgctg
      961 aatgcgaaag tagtgatccc gttccaccac gatatctggt caaacttcca ggccgatccg
     1021 caagagatcc gcgtgctgtg ggagatgaaa aaagatcgcc tgaagtatgg cttcaagccg
     1081 tttatctggc aggtcggcgg caaatttacc tggccgctgg ataaagacaa cttcgagtac
     1141 cactatccgc gtggtttcga tgattgcttc actattgaac cagatctgcc gttcaagtcg
     1201 ttcctgtaat ctgtcgcttg taccggatag cgtggagatg ctcgcgctat ccggcatagt
     1261 atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa tagctcatga
     1321 ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc tttgtgaccg
     1381 ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcacgacgc gatatcaata
     1441 aacttgacga aagcggcaaa ctgaaaaaag tgcgcaatgg cgcagaagct attacccaac
     1501 agcgcccgcg ctggacgccg atgaatctgc atcaagcgca gaatcacgat gaaaaagtac
     1561 gtattgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc atcaactgcg
     1621 gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta
     1681 attatctacc gctggcaaat tacctgatcg atcaggagca tgacagcgtg atcattatgg
     1741 gcggacagta caacaaaagt cagtccatca ctttaagccc gcaaggcagc gaaaacagtc
     1801 tctatgccgg gcactggatg tttaccagcg gcaaaggact gacagcagaa gggttgtata
     1861 aaaccgatat gctgacggca atggcagagc agaagatgct gagcgtggta gggaaactgg
     1921 tggtactggt tgatagcagt aagattggcg aacgcgcggg aatgcttttt agccgtgccg
     1981 atcaaatcga tatgctgatc accggcaaaa atgctaaccc ggaaatcctg caacaactgg
     2041 aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag
     2101 catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa caggttagca
     2161 gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg tcggcaggca
     2221 cgacgtcatc gtcaaggcca tgccataaga gcagcggtct gttccccaac tctgtcagat
     2281 ggtttgtcgc ttcccactct gccagcggcg cgacaatgtt attgaattca ttctgctgtg
     2341 ctgccgtttc aggtatcagc ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg
     2401 atcccatcat gctggcggta cattttaccg tggggtggcg agcggtaatt cccagtgccg
     2461 tcatcgcgcc catcgacgcg ccaccgactg ccagacggtc atcacgcagc cagttttctt
     2521 cggctattgc cgcacgtaag gtagtgaatt cctgcatact ttgtagcaag atttgccaga
     2581 attgatttaa ccgccgcgct gcgtcgccac taaaacggct accgtgatcg ggcgcatccg
     2641 gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta tacaccagac
     2701 tggacgaagt aaagccgtga taaaaaatca cacacggtaa cggggtatct ttttgcccga
     2761 caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt tcaatcatct
     2821 gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt ctgctggcgg
     2881 tattagttca taatgttgag atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg
     2941 cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg cactacacta
     3001 tggttagcag gaaacgtaat agcattattt accggtttgc cagtttatta ttggtgttga
     3061 tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca gtaacggacc
     3121 atccgcaaga gattcgccgt gaccagacgc aagggttaca gcgaataggt agcgtaagca
     3181 cgatggttcg aggttctccg gatgacgcat tagcagaaat cagagcgaaa gctgtcgctg
     3241 caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt
     3301 attcacaagc cattttgtac cgtaaataaa tcagacaact aacatagtca gctttacatt
     3361 gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa gctcgggcgt
     3421 ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga gctgacgatg
     3481 aaacaattac ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag cgccacggca
     3541 cagtctgcag aattcgccag tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc
     3601 tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc
     3661 gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca gcagcctgat
     3721 aactggcgcg tacaggcgat actgtatgcg tgagtgtcat ctacagcaaa tgaacaatgc
     3781 cggatgcggc gcgagcgcct tgtccggcct acaattttaa aatatttcat ttagttcatc
     3841 gaaaccagcg tcgcatcagt cgtatacgct tacaaacaca ctcctcccgt cgcccgcagc
     3901 aataaatcat tctggatctg ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc
     3961 ttacaccacg tctgcgccat tggcggagag gcatatttca acatttgcgc accgcagccc
     4021 agcaggaata gctgatgagt aatctctcgc cccaattctt cagctggctt acgcagctgc
     4081 tgctgtaaac gacgaaccgc gcgatcaaaa tagcgatcct gccctttcac ttccacaaat
     4141 gcttccgaca ataagtcgta tacgcccgct tgcttattga gaacgcgcag cacatccagg
     4201 cacataatat tgccggaacc ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc
     4261 ggcagctcgc tctcctcgca ataaccaatg ccgcccagca cctccatcgc ttcggccaca
     4321 aacggaatgc cacgtttgca gatcacaaat ttcgccgcag gcgtaaataa acgcgcccac
     4381 agagcttctt tggcatcggc gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc
     4441 gtttgccctt caagctgaag tgccatgcga cttaagacat gacgcataag gggctgttgg
     4501 atcaatggat taccaaaaac atggcgttga tgtgcatgat aaatcgccag cgaaaatgca
     4561 cggcgcatca tggcatggct acccagggcg caatcaaaac gcgtcatccc acccattttc
     4621 aggatcagac gaattccttc cccttcctgc cccaacaacc agccaatggc atcctgaaat
     4681 tccacttcgc agctggcgtt agagcgatta cccagcttat ctttcagccg ctcgaggcga
     4741 atcgcgttgc gttgcccgtc aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc
     4801 gcggtctgtg ccagcaccag atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc
     4861 cccaccagcc gataagaacc atcgtccaga cgctctgcgc gggtggtgtt gctcatgaca
     4921 tcggaaccgc cctgcttttc cgtcattccc atgccgatca acaagccacg tttttgccca
     4981 cctggcaata agtgagaatc gtagcgatcg ctcaacagcg gcgtggtcca gtcctgaaac
     5041 ggcgcgggta acatctgtaa caacaatggc gtggcggcaa aggtcatcgt tatcggacat
     5101 aacgacccag cctcaacctg cgcatgtaac ataaaacgcg ccgcgcgcgc cacaaatgcg
     5161 ccggagcgag cgtcttcttc ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc
     5221 atcagcaggt gccaggcggg gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg
     5281 tagcgcaata attcaggcgg attcacattc gccagccgcc ccagttcaag ggattcagcc
     5341 gttcctaact gctgaccaat actggcgaga aaatcgctat cccagccagc accttcacgc
     5401 gttaccgctt cgcaaagcgc gccatcagac aggtataagt tgctgttatt taatggtata
     5461 ggttgattaa aaacggtgtg agtttgccag tgcactgtgt ctccctccat caatggcagt
     5521 caccattagt atggtcactg ccatggattc atgacagaaa gagattgcta aaacattctt
     5581 atccagcaat gtaatgcgga acgaaacgtg aggtgtcttt ggtgatcagc gtgttatctt
     5641 cacggatccc cattccgcac gcttcatcat cgacaatcca gctaccgatg agtgtgtagc
     5701 tatcgccaaa tcgcggcaga ggttgaaacg cctggtagat catcggttca tcggcgtaat
     5761 caccatcagc gtggtcaaca acgttattct gaccgtcgaa aatggtgacg tttccgcctt
     5821 cgcgcgagta aatcggttta cgcacatagc tttcgccagc ggcaatctgc ggtttttcac
     5881 catcgaacca ggatgcaaga aggttaggat gaccagggaa gaagcgccaa agcagcggca
     5941 ttagcccttt attactcaag atacttttcc ataacggctc cacccattgc tcacgacgct
     6001 tgcgcagcag cggaccgtta tcgtcacgca tcatccactc cagcggatac agcttaaatg
     6061 cacgctggat gacattatca tcaagatcgg tcagtacgcc gccgacgccc aaaccgagct
     6121 cttcaatgta gataaaccga gactcctgcc ctgcctgctc ggcgcagtcc tgcaagtaca
     6181 gcacggtact cctgtcttca tcggtatcct gacagcagca aaaataaaac ggctcccggc
     6241 tataaagctc gctaaagcgt gaaatcaggc gctcctgaat ggcattgtac tgatcggcat
     6301 cgcgcggaat aacgccgctg cgccgggcat cttccagcca caaccactgg aaataagccg
     6361 actcgtacaa tgaagttggc gtatcggcgt tgtactccag caacttaacc ggtgcattac
     6421 cacaccaggc aaaatccata cggccataca acgaaggatc gcgtgcgcgc cagctttcgg
     6481 cgatcacatc ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcatctttaa
     6541 ccgcacgatc caccacctcc aggcacatct gatgcagttc cgcagtcggt ttttcgatct
     6601 gctcttcaat ctggcgcaga gtaaagcggt aagcccgact ctcatcccaa tagatttcat
     6661 tgtcgatgat atgaaagtca aatccgttat cggcggcaat ccggtccaga tcccgccgca
     6721 caggaacgtt gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg
     6781 accatagccg ccgcgagaaa cggttgacgc ttttttggtg gtaaagccgc ccgaagagta
     6841 agactctttt ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgcgccagac
     6901 ggggcgggaa gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt
     6961 acgaataacc cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt
     7021 gtcgtagtaa caattttcat acttagactg acaattctgc tgagtcatat tcttcggaac
     7081 atcggcataa aatgccgctt tggcgttgtt ccagccatga gcacagatat ctgaattatt
     7141 accatcatca atacaatcct gcacagtcgc gtaaaacgtt ccgtcgccgt cgttatcgac
     7201 atcgccacta tcgccacaac ctttcaatac gaaaaaagcg gcaccgccca ttagcgccaa
     7261 cgttaagtat ttttgtgcgt agcgattgcg gcgcggctca aaaggattat gcggtctacc
     7321 aatgcgactg attgccccat gtccgatatt tttgttattt ctggatttgc gttttctggc
     7381 catgatcgtc cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc
     7441 ggttcccata aacattccgg ctgcggtatt gtgattaata attttttcgc ttaatgcggg
     7501 catataaagc ctgacgccag caaaaacgag taattgaatc accagagcaa tcccgcccca
     7561 tgcgaaatag tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta
     7621 ccccaacaat gttccgctga acgccagtgc cgccgcggtg ttattgtttt tgattaactg
     7681 ccattcgttg tgcggtgtaa ttttggagta gataaacagg aaaataatca ccatcgccac
     7741 gccaataaaa aaataggcac taaaagcaag aagtgaatcc agtatatgca ttgtaaacgt
     7801 ccttgtaatt aaccaataat atgaagtgat gtcagcggga tatcgatacc cagcgcgcgc
     7861 gaaaataacc actccggctc gccgagatcg ttaaaagatt cctcaccatt taacagcaaa
     7921 tattcgtaag tatctccggt cacctgccgt tggtagccca tgctaaaatt atgcacttcc
     7981 catttggcat ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc
     8041 ggttcttcgc tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc
     8101 cccatcgctt tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc
     8161 tcttcataga caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccagtggta
     8221 ttgatttgca ggaactcatc gcccgaagtg tagtaacgga aaatctggct acccccgccc
     8281 agatcgatgt ggctgacagc ggctaccgta aattcctcac ccggcagcgc aatcagcaat
     8341 tcatcttcca gcaaacgaaa cgataatgtg tcgagcgtaa agccactatt gagatgcaga
     8401 cccagcggac cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa
     8461 ccagacatat tttatcctta aaatagttac cagccttgca gtgaggctgg caaaaaatta
     8521 ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc
     8581 gccaattcca gcttccgcca gcttttcatc gagatcgcga ccgtccgcga ctttttccaa
     8641 ctgtgcggca gcatccaggc gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa
     8701 ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg gtagaggtcg ttacagcctg
     8761 ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc
     8821 gatacgctgc gctgtcgctg taactgcttt ttccacgcca tcacgagaga cttccagatt
     8881 cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac
     8941 ttcgttaatc aacgacggat taacgttctt gcttaacgct tccagcgcac gcgcttccag
     9001 actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca cccgtgccag
     9061 cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg
     9121 ttccagcatg cgcacgccct gggtttcttc aatggactct tccgcctggg agataaacga
     9181 tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg
     9241 tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc
     9301 aagcaggata tcttcaagag aagatttaag cgataacgca ccgaaaacaa tgtaatactc
     9361 ttcctgttgc acgctggaga tccctaccga tgacaacggc atcatcttct gatttcttaa
     9421 taagaaggta ttaaattcat ctggattgct gatactactt accggacaaa taaaggtttc
     9481 gatgatcatt tggcgggaag taaaaagaat attgatttgc aaatcgccat aatcattcag
     9541 tttaataatt aatgcacttt cgttatttgt tacatcaata ttttgttcag gtacagtttg
     9601 cagcgccgtc gccagcgcca acggattcca tgtcatatat tattccatat agattaagtt
     9661 taaatattaa taaaatgaat atttgcagta cgtaattatc ttaccagcta tagacaaaaa
     9721 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
     9781 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
     9841 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
     9901 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
     9961 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
    10021 cgccagatta gtcacccgaa tcagcggaac gctttctgcc gcaccacagg ccactttttt
    10081 cgccgtggcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacaccagc
    10141 ggcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
    10201 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
    10261 ccgtcctggc ttcacacgag cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
    10321 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
    10381 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
    10441 ggcgcgctcc agcagggcct gcactgcatg gatgccgtaa atcatttcgc tcattaatgt
    10501 actcgttgtt tacgttttgc cgggagcgcc acgcttgccc ggcctacgga taattcaccc
    10561 tcttctgttt gaagagggta atgatcactc tgccactttt ttcttcgccg cacgcttcgc
    10621 tttggtcgct gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc
    10681 tttcttcgct gctttcggct tcggtttttt ctcgccacgg aaggcgctgt ctggctcaaa
    10741 gttgaccttt ttgccgacct gacgccgctt gccgcctttt ttgcctgcat cgcctttttt
    10801 cgctttctcg cgcgccgttt taccgacgtt gcgcggtgcg cgttcgctgg agatcaggct
    10861 aaagtcgatt ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc
    10921 caggcgataa gtctgaccgc tggattcacc catcaggcgt tgccctacct ggtcaaagcg
    10981 atagtagtca ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag
    11041 acggacgaag aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac
    11101 ctggtcgagc atgaagtcac acttcagcca gtcagacaca tcgcgcgttg cttcatcggc
    11161 acgacgttcc gccatcgaac agtgctgacc cagttgcagc atctcttcca tcgaataatg
    11221 gtagccgccg gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatattt
    11281 aatggcgcgg tgcaacgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata
    11341 ggactgcaat gccagaccga agtgaccacg gttttccgga tcgtaaatcg cctgtttcat
    11401 cgagcgcaaa agcatggttt gcagcatttc tgcatcagga cgatcggcaa ccgattccag
    11461 taactctgca tagtcgcgcg gttccggctt gttaccgccc ggcaattcca gccccagctc
    11521 cgccagcact gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa
    11581 cagtgccggt tctttggctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat
    11641 gcactcttca attaacttgt gggcgtcgtt acgctgagtc tgttcgatac gttcaatacg
    11701 gcgttcagcg ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc
    11761 ttcacgggct ttatccagca ctttatagag gttatgtaac tcttcgagat gcttaaccag
    11821 cggcgcatac tgctcgcgca gatcctgatc gccttgcagt atatgccaga ctttggtgta
    11881 ggtcagacgc gcgtgagaac tcatcaccgc ttcgtagaat ttgtagccag tcaggcggcc
    11941 tttcgacgaa accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga
    12001 gcacaggccg ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac
    12061 cgacgtacca cggttacgcg cttctctgtc cagcggtgtt ggcggacgga catagtaact
    12121 gacgtcggca attgcgaccc acaaacgcca gccaccgccg cgttttttct cacagtaaac
    12181 tgcatcgtca aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcag
    12241 atcgacacgg cccgcttttg cttcttccgg cacttcttct ttcagcccgg caacctgttg
    12301 ctcaacagcc tgcggccaga tgtacggaat ttcatgggta cgcagagcaa tatcaaccgc
    12361 catgccggtg cccatattat cgcccagcac ttcgacgatt ttacccaccg ctttggtacg
    12421 gcgagtcggg cgttgagtca gttcaaccac caccacaaag cccatccgcg cgcccatgat
    12481 ctgatcgggc gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc
    12541 gacgcccgct tcggtgaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg
    12601 gacaatacgc gcttcacgac gacctttacg gtcagcgccc agcggctgag ccagcacctg
    12661 atcgccatga atgcaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc
    12721 ttcaactcgc agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc
    12781 gaggcgttcc ggcagcgcat agcactgacg gcgagtgaag accagttgac catcgcgctc
    12841 catcgcgcgc aggcgtcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc
    12901 taccgccagc tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa
    12961 ttcccgacta gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg
    13021 tgacatctcg gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat
    13081 aatttataaa gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc
    13141 gtcagaaaac tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg
    13201 tggcaaaact cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca
    13261 ccaatacgta tcgcactcgc cggtttaccc aagcgaatgc cgccattttt tccacgaaca
    13321 gcagtcacgt agccggcacg actaagttga ttgattattt tgaccatatg attacgggag
    13381 acgccgtaga cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc
    13441 atgtagatca gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca
    13501 aaagggaaat cggggaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg
    13561 ctaatttatt tcagttcggg gaaggaaaaa aacgaggatt taacactgtg tcggatagcg
    13621 taatggctta accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc
    13681 catgcgtacc agaattacgc gtcgaacggg tcgcgcagaa tcatggtttc agtacgatcc
    13741 ggaccggtag agatgatatc gatcggcaca ccggtcagct cttcaatgcg cttgatgtag
    13801 ttcagtgccg cctgcggcag gccactacga tctttcacgc cgaaggtgga ttcagaccag
    13861 cccggcatgg tttcgtaaat cggctctaga cctttccagt cgtcagctgc cagcggagtg
    13921 gtagtcactt cacgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg
    13981 ccatccagaa cgtccagttt agtcaggcag aagccagaca gggagttcaa ctgtaccgca
    14041 cgacgaacgg caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg
    14101 aattcgttac cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac
    14161 ggacctgccc ccacacgagt ggagtaagct ttgaggatac ccagaacgta atcaacataa
    14221 cgcgggccca ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg
    14281 tacggatatg taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg
    14341 aaatcgccac gctgacgcgc ctggtcgagc aggtcagaaa cgtcaacaac catagaagtc
    14401 aggatgtcgg caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct
    14461 ttgtagtagt taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg
    14521 aaggtttctt tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca
    14581 taagcaggcc cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca
    14641 cgcgcgttat ccagcgcaac gtgataatca aggatcagcg gacatgcttc ggacagcagc
    14701 agacgctcac gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgca
    14761 gccggagaca gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct
    14821 gatggaataa gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg
    14881 tgaccgccct ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta
    14941 cctttacctt cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa
    15001 aatcaccgtt tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt
    15061 gcactcaaaa atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat
    15121 gtagtagacc acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc
    15181 gggcaaattg gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc
    15241 taaaccttcc agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta
    15301 tccttataga aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt
    15361 accgcagtcg ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc
    15421 gctgtccggg ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg
    15481 caggctacgg atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt
    15541 ggctgcttct gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag
    15601 cgttctggtc acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg
    15661 tgaacggtga cgacgtgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc
    15721 agacacttcg gtcggcaggt tgatctgctt aattcgcaca tcgacaactt caatacccag
    15781 cgccgccata ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg
    15841 ctctgccgct tcggcaatgg cgttatctgc tgcaggggta gtaacttcat cttctgtacc
    15901 cgcagaaccg gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt
    15961 gacgatatct ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg
    16021 tttcaacagc acttccgctt gcgaaatgtc gccaccaccc gttgccagat agtaacggct
    16081 gaaatcgctg atgcgccatt tgatgtaaga gtcgacgatc aggtctttct tctctttggt
    16141 cacaaagcgg tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt
    16201 ttcaatgaac ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc
    16261 gtcacgcagt accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa
    16321 gacagacatg taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat
    16381 cgttattccc cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata
    16441 atatcgccct gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct
    16501 ggcggcagac gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttacca
    16561 cctttcagca tctggtctaa tggcagaacc atcaggttgc cacctttatc gttaaccagc
    16621 actttgcggg tgttgcccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta
    16681 atttccggcg cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga
    16741 gcttccagga tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga
    16801 ccgttcgcac gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt
    16861 tcgttttcac gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga
    16921 gcagcctgga agttgacgtc cagcagcgtg atacccatgt catacggacg aatcgtctct
    16981 tccagttcgc gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc
    17041 atggtgtatt taccgataac cccgcgtagg gcgctgtcgg tagcctgacg caggctgtca
    17101 tccgggctgg tcacgctata cagatatttt tccggatcgg tgacgcggta ctgcacgttc
    17161 atctcaacgc gcactacgtt ctcgtccgac gtcagcatca caccagaagc tgccagttca
    17221 cgcacggctt ccacgttcac cggtttgact tcgtcaataa acgtcggttt ccagttcaga
    17281 cccggctcaa ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct
    17341 ttaatggtat agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta
    17401 acgacgcgac cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg
    17461 gtgcctttac cgccacccag accaccgagc tttttgctca gtttgcggaa gatatcatct
    17521 aaatcaggtg gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca
    17581 ggtttgctgc ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc
    17641 atgtttgtgc tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata
    17701 tgactccacg cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg
    17761 tttacagagg cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc
    17821 ctcctccatc cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgccc
    17881 ttcctgcggc ggcagacgca atgtatgctg cgccacctcg ccggaaagac gctccgtcaa
    17941 agcctgaaaa agctgtggta tccccgctcc ggtctgcgca gaaagccaga cacggatcgg
    18001 tttgttctct tcatctctgt caatacgcgg ttcgaaatcg tccagcatat cgatcttgtt
    18061 catcaccagc agggttggga tctcgtgagc atcaatctct tcaagaaccg tattcaccgc
    18121 ttcgatgttt tcttgtacac gcacatccgc cgcgtcaatg acgtgcagca gcaatgtggc
    18181 ttgccgcgtc tcttgtaacg tggctttaaa tgccgccacc agatcgtgcg gcaggtggcg
    18241 aataaaccct acggtatctg ccaacacggt ttcaccgaca tccgcaacgt caatacgccg
    18301 caacgtcggg tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat
    18361 gcgattgaaa agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac
    18421 atcggctttg atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttccaggcg
    18481 cgactgtatc tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc
    18541 acccggacca cgcaaaccga tcccgccttt ctgtctttca aggtgggtcc agccacgcac
    18601 caggcgcgta gccagatggc gcaactgcgc cagctcaacc tgcaacttac cctcatgggt
    18661 acgcgcacgt tgggcgaaaa tatctaaaat aaggccggtg cggtcgatga cacgacactc
    18721 gcacaaacgc tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac
    18781 cgaagcaccc gtcgctttta cggcttccgc aatttcaact gctttacctt cacctacaaa
    18841 atactttggg tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc
    18901 ggaagagacc agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata
    18961 gatgtgtacc agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct
    19021 ctaaatagat cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa acagcccgaa
    19081 accttattcg gtttcttcgc tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg
    19141 atgatagtta ctgctggtac cgccaccggc gttgttactg tgatgagaaa ccgggcgaga
    19201 cgggacaaca gtagaaatcg cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag
    19261 gatcacgaac tgatcaaaag actcgatttg tccttgcagc ttaataccat tcaccaaata
    19321 aatagaaact ggaacacgtt cccgacgcag tgcgttcagg aacggatctt gtaaagattg
    19381 ccccttagcc attctctctt ttccttatat gcttatttgt actttgaacc tttcgattct
    19441 gaaaaaattg cgcacgatac gtctcaattg tacacattca gcctgcgata gcaccaacaa
    19501 cctgtaatac ttcgtcacgc gcctgttctg gtttttcact gtcaagccag tgaacccctt
    19561 cccaaccacg cagccaggtt atctgccgct tcgccaactg tctcgtggcg caaacacctc
    19621 gataaaccat ttcatcgtat gagatttcgc cttcaaggta agaccacatc tggcgataac
    19681 ccacgcaacg aatggaaggc aaatccgtat gcaaatctcc tcgggcaaaa agcgcccgga
    19741 cttctgcttc aaaacctgaa gccaacatct gatgaaaacg ctgctcaatt cgttgatgga
    19801 gcagttcacg gctcgccggg gcgatggcga actgatgcac ctgatacggt agagcgtctc
    19861 ctgacgtttg cgtcagttcc gttaaagttt tacccgaaat gaaaaaaact tccagtgccc
    19921 gggaaagcct ttgtggatca tttggatgaa tccttgctgc cgcaaccgga tctacctcct
    19981 gaagttgacg atgcaatgac tcccaacctt gctctgccgc ctgttgctca attctggccc
    20041 gtacttccgg gtccgccgac ggtagcggcg acaacccttc cagcaatgcc ttgaaataca
    20101 acatcgtacc gcccactaat agtgggatcc gccccgccgc ggtgatatcg gccatttccg
    20161 ccagcgcatc gcggcgaaaa tcagcagccg agtaagcctg cgacggatcg cgaatattca
    20221 gcaatcggtg cggcgcggcg agtaactctt cagcgttcgg cttcgccgtc ccgatatcca
    20281 tccctttgta aataagggca gaatcaacgc ttatcaactc tactggtaaa attttacgca
    20341 gctcaatggc taacgctgtt ttaccggagg ccgtcggccc catcaaaaaa atcgccttag
    20401 gcaggctcgc cttactgata tcactcatct ttcagggctt ttatcgccgg atgtaaatca
    20461 acagattgta acagaccacc cggcggcgtt ttcacaagtt gcggacataa ccgttccacg
    20521 tccgccagca gggttatggc ctgtgccatt gaccactgcg catgttcgct catcagattt
    20581 cgtgcaatcc actgcgcaat attgccaggt tcgaatacgg actgcttcgc caggtagcct
    20641 atcagttcag gaatcaagat ttgtaaattt tgttggcgta agggtaaagg cacggccctg
    20701 atggtcacat gctgcgcatc ggactggaaa tcaataccca attccgccag ggcagactgc
    20761 gctttttcta atgccgattt ttcttcgcca gaaactttta gccgcaacgg aataagcagc
    20821 ggttgggcgc aaactggcac ttcacccggc gtcaattgtg cctgacgcag ccaacgttct
    20881 gccactggca aggataaaag tgaaatgttg ccatcgcgct caagcaacgc acagtcggaa
    20941 tggacgatag tcagtacccg accaaaactc tgactgttcg ccgcaagtgc aggttcctgc
    21001 ggttctggcg cttttggttt ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact
    21061 tcaccttgct gtttctggta acctggctgc gcattcggcc agggggcagc cggacgactg
    21121 cctgaggagg gcgcaggagt gtaacgcgga gctactggct cacgaactgc cggttcagca
    21181 aaatgattgc gccccgccgc cacgcgattt tccggaatgg cacgcggtgc aggttgtggt
    21241 tcatcgtcca gcggtagcgg cgtttccagt tgctgttgta gcacgctcag cacgccctga
    21301 tagataaagt catgcaccag acgcgactga tggaaacgca cttcgtgttt ggcagggtgc
    21361 acgttgacgt ccacctgatg cgggtcgatc tccagataca acacaaatgc cggttgctga
    21421 tcggccccca gtttgtcttc gcaggcctgg cggatcgcgt gattgatcag gcgatcgcgc
    21481 atcatgcgac cgttcacgta gcaatactga atttctgcca gtgcgggcgt ggtgtgattt
    21541 ggatcggcca cccagccgcg cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct
    21601 tgttcgagaa aagcggtgcc gcaaatcgcg cctaagcgcc gttctttttg cccgccttcc
    21661 ggcactgcgc ggtactggcg tacaattttg ccgttatgcg acaggttgat agtgacgtcg
    21721 aaacgcgcca gcgcaatgcg gcggatgatt tcatcgatat ggttaaattc ggttttctcg
    21781 gtgcgcagga atttgcgccg cgccggggtg ttgtagaaca ggtccagcac ctccagcgtc
    21841 gttcccaccg gatgcgccgc cggtttaacc gtcacgtcca tattgcgccc ttcggcataa
    21901 gcctgccagg cttcctgctg ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa
    21961 ctgatactcg ccagtgcctc accgcgaaag cccaggctga taatggcttc gagatcgtcc
    22021 agagaggcga ttttactggt ggcatgacgc gccagcgcca gtgccagctc atcttttttg
    22081 ataccgcagc cgttatcacg aatgcggata agtttcgccc caccgcgttc gatatcaata
    22141 tcgatacgcg tcgcacctgc atcgaggctg ttttccacca gttctttgac taccgacgca
    22201 ggtcgctcga ccacctcacc tgcggcaatc tggttcgcca gttgtggcgg taagacctga
    22261 attggcatca atcgtcctta gtttggcagc gtgcgatctg gcgtcgtcac cgtactggcc
    22321 gtttgtgccg ttgcaccctg cggcgcagat tgcatcggat gcgcaaggaa gtaattgcgc
    22381 aggcctttgt aaatggcttc tgccagctgt tgttggtaat cgtcgctcgc cagcaaacgt
    22441 tcttcgctgt tgttactgat aaaaccggtt tcgaccagta ctgacggaat atccggcgaa
    22501 cgcagaacgc cgagactcgc gtgttctggt cggcgtttat gtatttcgcc aatgcgttgc
    22561 aactgactga tcatactggt cgctacatca taccctaccc gctgggaatg accgaactgt
    22621 aaatccagca ccgcctggct taaatagggg tcggcctgac tgttcgccag cacatcaccc
    22681 gccccgccca gcagctccga ctgcttctca tgctgttcca gccagctggc catttcgctg
    22741 ttggcgcgac ggtttgagag cacccataca gaggcaccgg tggcactacg gtttggtgcg
    22801 gcatcggcat gaatcgacac aaggaaattg gcgttttgct tacgtgccac atcactgcgc
    22861 cccatcaccg agataaagta atccccgtca cgggttaaaa cgcccttaaa catcggatcg
    22921 ttattgagca aggtacgtaa tttacgcgca atggcgatgg tgacattttt ctcccgtgta
    22981 ccaccagggc cgatagcgcc agggtcctga ccgccgtgtc cggcatcaat agcgatgata
    23041 attttatcgc ctgtgttagc cgtcgcgcgc gctgccggac gcgttaccgt attactgctg
    23101 ataacacccg tagtgcggtt actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg
    23161 cgcggtgcga caaccgcagg cgtttcaacg cgtttcgcaa ccattggcgg aggcgatggc
    23221 ggcgcatcgg cgttaatcgt aaagacaaca gtgtaattgc tgccattctg ccgcttcacc
    23281 gcttcggttt taccgttttc ggtgagatcg accaccagcc gtagcgtttg tgcatcttta
    23341 ggcgttccgg agcgaatcgc tttcaccagg ttattaccgc taaacaacaa cggcagcccc
    23401 tgaattaccc ccgtttgttt gatatcgagc gccacggtgc gtttgctttg atggctaaac
    23461 gcataatcag gatcgccaat aaaactcaac gttatccgcg cctgttgatt accgttagaa
    23521 acctgaatat cagagagcgt cgcggccccc gcctgcgcgc acagcagcag caacgtcgct
    23581 actaaccaat ttctgatgcg atacatcatc ccgccacctt tcaaaggtta accggctaaa
    23641 cgcgccagca acaattcacc cgcagaggaa accgcactca cgcgcgcctc acggccttgt
    23701 gcctgataat caatgtgtat ttcgacatcc gggtcaggaa gaacacctgt accttgttgt
    23761 ggccactcca ccaggcagat ggcatcgttg gcaaaataat cgcggatccc cataaactcc
    23821 agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc
    23881 gtatagggtt cgaccagcgt ataagtgggg cttttgacat taccctgatg acccagagcc
    23941 tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta aatcgccata cagatagatt
    24001 acggttgcgc catcgcaggc tttcgctacc cgctcgccca ggtctaatgt tgcctgctca
    24061 tcagggagcg gaattactcg attcatcatg gtttttatca gtcacttccg ggttaacaat
    24121 acgctgtagc gtggaaaaga gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc
    24181 cagtacgtca gctgccgcac cgtgcgcgac acagcccgca caggctgcat cataagggct
    24241 cattttttgc ccaagcaatg cgccaataat accagagagc acatcgccca tgccgccgct
    24301 cgccatgcct gcatttccgg catcaataat gcctaaagcg tcaggatggg cggcgaccac
    24361 ggttccggca cctttcagca ccgctacgcc gccataacgt tgtaccagac gttgggcgca
    24421 atgtaagcgg tcactttcaa tttcagcgac ggaacatcct aacaaccgtg cggcctcacc
    24481 aggatgcggc gtgatcacgc gattgtgacg cttatcggga ttgattgcca gcaggttcaa
    24541 tgcatcggca tcccacaaca tcggtttgcg aaaattctca actttttgca gggctttttt
    24601 cccccactct tgctggccca gaccgggacc aatcaccacc acatcggccc attccaggct
    24661 ttcggtaaga gagcccatag tcagttcatg caccatcaat tccggtcgtg cagtcagcag
    24721 cggcgcaatg ttctcactgc gggtcagtac tcggactaaa ccagcaccag cacgcagcgc
    24781 cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga tcgccaccaa taatcaccag
    24841 ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt atcagccact gagaaagttg
    24901 ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc agccatctat ccagccgcag
    24961 tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt ccagtgagca aacctggttt
    25021 cagcgcaata aaagtgatgg tgtgatcggc gttgattacc gcgcctggcg tagcgccggt
    25081 ttcagccaga aggccggaag ggatatcaac cgccacaatc ggcgcaggat gggaattagc
    25141 gtggtcgatt aactggctaa tggattcgcg gggcgcttgc tgcaaaccgg tgccgagcag
    25201 cgcatcgaca atcagatcta ccgattcggg ccagacaata ttcgaagcat ggatctcgcc
    25261 tcccgcgttt aaccatgctt cgcgtgccag cgcggcctct tccggcaacg gtttgtcact
    25321 ttcctgggcc agcagcgtga catcaatacc gatcgctttg gccagccgcg cgaccacgta
    25381 gccatcgccg ccgttattac catgaccgca cagcaccagc cagtggcggg cgtcaggata
    25441 agccgaacga cacacctgga atgcggcttc gccagcgcga agcatcagct catagagtgt
    25501 gagccccagc gcatctgccg cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac
    25561 ggtgtgtggt atacttacgg ggtttttctt cattgtatgg tccgtcatgt cagagcccct
    25621 cgatctcaat cagttagcgc aaaaaattaa acagtggggg ctggaactgg gctttcagca
    25681 ggtaggtatt accgataccg atctcagcga gtccgagccc aaactgcaag catggctgga
    25741 caaacaatac cacggcgaaa tggactggat ggcgcgtcac ggtatgctgc gtgctcgtcc
    25801 tcacgaatta ttgcccggta cgctgcgcgt gatcagcgtg cggatgaatt accttcctgc
    25861 taacgccgca tttgccagca cgctgaaaaa tcccaaactc ggctatgtta gccgttatgc
    25921 gctgggccgt gactatcaca agcttctgcg caaccgactc aaaaagctgg gcgagatgat
    25981 tcagcaacat tgtgtttcgc tgaattttag accgtttgtc gattctgcgc ctattctcga
    26041 gcgcccgtta gctgaaaaag caggtcttgg ctggacaggt aagcactcac ttatcctcaa
    26101 tcgcgaggcc ggttcgttct tctttttagg cgaattgctg gtcgatattc cgctgcccgt
    26161 ggatcaacca gtcgaggaag gatgcggtaa atgcgtggcc tgtatgacga tttgcccgac
    26221 cggtgccatc gtcgagccat ataccgtcga tgctcgccgc tgtatctctt atctcaccat
    26281 cgaactggaa ggggagatcc cggaagagtt gcgaccgtta atgggaaacc gtatttacgg
    26341 ttgcgatgac tgccagctta tctgcccgtg gaatcgctat tcgcaactca ctacagaaga
    26401 cgatttcagc ccgcgtaagc cgctacacgc accggaactc attgagttat tcgcctggag
    26461 tgaagagaag tttttaaaag tcacggaagg ttcggcgatt cgccgtatag gtcacctgcg
    26521 ttggctgcgt aatatcgccg tagcattagg caatgccccc tgggatgaaa cgattttgac
    26581 agcgctggaa agtcgtaaag gtgagcaccc acttcttgat gagcacatag cgtgggcgat
    26641 tgcgcagcaa atcgagagac gaaatgcgtg cgtggtcgaa gtgcaattac cgaaaaaaca
    26701 gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac gcctgattca tccacagctt
    26761 gtgaataaaa ataaaaacgc attgcaattc aagccggaaa aaatcgtcaa gtgatcgata
    26821 cgacaaaacg aaatcttaat ttttaactta tctatcaata ggttaaaaaa atactattca
    26881 ccgtgcgaag tttctttgaa attaagatta cggcctgagg ctgtggataa ctctgtttac
    26941 aagagtttgt cagagacaac aaaaaccagc aataacgcat cgcgtcgctg tggatatttt
    27001 attgagagaa gaatttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    27061 ggtcgtgctc taccaactga gctattcccg ctttggtggt ctgtgcttta cagcactttc
    27121 aaattttgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct
    27181 ctaccaactg agctattccc gcttgggtgg tctgtgcttt gcagcacttt tcaaattttg
    27241 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
    27301 tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt ctgcgtcgtt
    27361 acgggaggcg cattatacga gaaatctttc cctctgcaac ccccctaaaa aatttttttt
    27421 gagtttttat ctcaagtgct gaaattatca gcaaggcgtt caatttagcg tcaaaatcac
    27481 gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag cttgataaaa
    27541 tgctcgcggt agtaagccaa ctccgccacc gattcacgga tatcatccat cgcctgatgc
    27601 gtcccctgct tggtaaagcc gtcaagaatt tccggcttcc agcggcgcgc cagctctttc
    27661 agggtgctaa catcgagata gcggtagtgg aagtaggctt ccagctccgg catgtattta
    27721 aacaggaagc gacggtcctg accgatgctg ttaccacaaa tcggcgattt tcccgcaggc
    27781 acccactgtt ttaagaattc gagcgttgcc agttcagctt cacgatcgcc catcgtgctc
    27841 gctttcacac gctctaccag cccgctggcg gtatgggtgc gcacgttcca gtcatccatc
    27901 agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc cagaatattc
    27961 aggttggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc gggatccaga
    28021 ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat gctattttcc
    28081 acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt tttgcccatc
    28141 aggggcgacc aggagtctgt acgattgagt aaaaataaac tctccaaagg ccagcagcgc
    28201 cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc cgactacgac
    28261 gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg tatgcacgct
    28321 gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac catccgttcg
    28381 ttggtgaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga aggcgtaaat
    28441 gtcaaaggga tcgtggaagc ggtgcatgag cgcacctcag tgttgacgcg cccggatttc
    28501 tacgacggtg tgaaacctat tgctgccaac atcgaccaga ttgtcattgt ctccgccatt
    28561 ttgccggagc tgtcgctcaa tattatcgac cgttacctgg tggcctgcga aaccttgcag
    28621 attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg catggagttc
    28681 gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat ggtttccagt
    28741 catactcagg atgggctgaa accgctggaa gaggcgttga ccgggcgcat cagcattttt
    28801 gccgggcagt ctggcgttgg caaatccagc ctgctgaacg cgttgctggg tctgcaaaaa
    28861 gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac cacgaccgcc
    28921 gcacgactgt atcacttccc gcacggcggt gatgtgattg attccccagg tgtgcgtgag
    28981 tttggtctct ggcacctgga gccggaacaa atcactcagg gctttgtcga attccatgac
    29041 tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg ctgcgctatc
    29101 cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta tcaccgtatt
    29161 ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga tgactgacaa
    29221 ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg taggccggat
    29281 caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa actttcgcta
    29341 cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc aagcaagcgg
    29401 gcaggatggc tgacaaaact ggttatcgat ctgttcgtta aatactacaa ggtcgacatg
    29461 aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt ctttgtccgt
    29521 ccgctgcgtg atgaagtacg cccaatcgat accgatccga atgtcctggt tatgcctgcc
    29581 gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca agccaaaggc
    29641 cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga tctcttccgt
    29701 aacggtacgt ttgtgaccac ttacctctcc ccacgtgact accaccgcgt acacatgccg
    29761 tgcaacggta ttctgcgtga aatgatctac gtgccgggcg atctcttctc cgttaaccat
    29821 ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat ctgcctgttc
    29881 gataccgaat ttggcccgat ggcgcagatt ctggtcggtg cgacgattgt aggcagcatt
    29941 gagacggtat gggcgggcac cattacgccg ccgcgcgaag gcatcatcaa gcgctggacc
    30001 tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga aatgggtcgc
    30061 tttaaactag gctctaccgt tatcaacctg tttgcaccag gtaaagtgga cctggttgag
    30121 caactggaga gcctgtctgt cacgaaaatt ggccagccgc tggcagtatc taccgaaacc
    30181 tttgttacgc cagatgctga accagccccg cttcctgctg aagagatcga agcagaacac
    30241 gacgccagcc cattggttga cgataaaaaa gaccaggtct aatccatcaa aggaaacgct
    30301 gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg ggcgtacgcc
    30361 gcgacggccc ccgatagcaa acaaatcacc caggaactgg agcaggcaaa agcggcgaaa
    30421 cctgcgcagc cggaagtcgt agaggcgctc cagtctgcct taaatgcgct tgaggaacga
    30481 aaaggttccc ttgagcgcat caagcaatat caagaagtca ttgataatta cccgaaactt
    30541 tccgccactc tgcgcgcgca gttaaacaac atgcgtgacg agccacgcag cgtgtcgccg
    30601 ggaatgtcta acgatgcgct gaatcaggaa attctccaga tcagcagtca gttgctggat
    30661 aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga ttccctgaat
    30721 caactgccgc aacagcaaac cgatgcccgc cgtcagttga atgaaatcga gcgccgcctg
    30781 gggacgctta ccggcaatac cccgctcaat caggcacaaa atttcgcgtt gcagtctgac
    30841 tccgcacgtc tgaaagcgct cgttgatgaa ctggagctgg cgcagctatc tgccaataac
    30901 cgccaggaat tagcgcgctt gcgctcagag ctggccgaaa aagagagcca gcagctggat
    30961 gcatatttgc aagcactacg caaccagttg aatagtcaac gccaactgga agcggaacgg
    31021 gcgctggaaa gtaccgagca actggcggaa agtagcgccg atttgccaaa agatatcgtc
    31081 gcccagttta aaattaaccg cgaactatcg gcggcactga atcaacaggc gcagcggatg
    31141 gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt ccggcaggcg
    31201 ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcat ctaacctgct cggcgaagcg
    31261 ttgcgggcgc aggtggcacg gctaccggaa atgccaaaac cacaacagct tgataccgaa
    31321 atggcgcagt tacgtgtgca acggttacgt tatgaggatc tgcttaataa acagccgctg
    31381 ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ctgagcaaaa ccgtattctg
    31441 gaagcacagc tacgcactca gcgtgagttg ctgaactcat tgttgcaggg tggcgacacg
    31501 ctactgctgg agctgaccaa gctgaaagtc tccaacgggc aactggagga tgcactgaaa
    31561 gaggtgaacg aagcgacgca ccgctatctg ttctggacct ctgacgtgcg cccgatgacc
    31621 atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct ggacaccttc
    31681 agtcagttgg gcaaagccag tgtgatgatg ctgaccagta aagagacgat tttgccgctg
    31741 tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt cacccgtttt
    31801 cttgaacgtt cggcggcgaa agtcggcaaa gtgactcagg atcacttctg gctgacgtta
    31861 cgcactcttt tctggtcgat tctcgtcgcg tcaccgctgc ctgtgctgtg gatgacgctg
    31921 ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg tgatggcgta
    31981 acagccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc tcgcccgaac
    32041 ggcttgttta tcgctcattt tggctggccg cgcgaacgtg tttcccgtgg gatgcgttac
    32101 tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat gttcgataac
    32161 ctcgacgacc gcgaattttc cggttcgctg ggacggcttt gctttatcct catttgcggt
    32221 gcgctggcgg tggtcaccct cagcctgaaa aaggccggga tcccgctgta tctcaacaaa
    32281 gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat tggcgcacct
    32341 ctggttgcca ttctggcgtc ggcggtgggt tatctggcaa cggcgcaggc gctgttagcg
    32401 aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca cgttatccgc
    32461 cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcaaagca tcgccgggca
    32521 gagatgttag cgcagcgtgc gcgtggcgaa gaggaagcgc atcatcacag tagcccggaa
    32581 ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc gcaatccttg
    32641 cggctggtgc gctcaatttt gatgttgatc gccctgcttt cggtcattgt gctgtggtca
    32701 gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt cacttccacg
    32761 gtacagggcg tagaaagcct ggagccgatt accctcggtg cggtgctgat tgccattctg
    32821 gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga actggcgatt
    32881 ctgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat caccaaatat
    32941 ctgctgatgc taattggcgg gctggtcggc ttctcgatga ttggtattga gtggtcgaaa
    33001 ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca ggaaattttc
    33061 gccaacttta tctctggcct gattatcctg ttcgaaaaac cgattcgcat tggcgatacg
    33121 gtaacgatcc gcgatcttac cggtagtgtg acgaaaatta acacccgcgc caccaccatc
    33181 agcgactggg accgcaaaga aattatcgtg ccgaacaagg cgtttattac cgagcagttt
    33241 atcaactggt cgctctctga ctcggtcacg cgcgtggtgt tgacgattcc ggcccctgcc
    33301 aatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg ctgctcgctg
    33361 gtgatcgaca acccggcgcc ggaagtcttc ctggtggatc tgcagcaggg gattcagatt
    33421 ttcgagctgc gtatttacgc cgctgagatg ggtcaccgta tgccgctacg ccatgagatt
    33481 caccagctga ttctggctgg cttccacgcc cacggtatcg atatgccatt cccgcccttc
    33541 cagatgcgtc tggaaagcct gaatggtaaa caaacgggga gaacattaac ttctgcgggc
    33601 aaaggtcgtc aggcgggaag tttgtaaggt ctttgtggcc ggatgaaccc atccggctca
    33661 caagcaaaac tgatagcgca gaaaaaactg ccacaaccag tagccgaaaa tagccagcgc
    33721 ggtggcgtac ataaaccagc cgcgtttttt tcgccacaaa gcgaaccaga gaaaagggaa
    33781 aaagagcggt aacgtcatta tcacaccgac atcgcgagta tcgtcatctt cgagatacat
    33841 caaaacatca cagacgttgt taatcccgcg tccatcaatc atccactctt tgtccagcgc
    33901 ggaggtaatg gttatccaga ggaacgccac aatgaaccag atgcagcaca agacagacat
    33961 gcgcgcactc atgaggtaac ccatagttta atttcgctat agcgcatacg aaatctcact
    34021 aattccctgt cattacgccc tatcgtcagg ccgacgatag ggcatttgat tactcagcca
    34081 cccactgcgg attttttacc actctgctgc aatagttctg gtaaatcgcc atcgctaaca
    34141 gcgagaagaa gacagggccg ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa
    34201 ccacaggttg aatgatggtg aagacgttcg caaatgtcac caccagtacg acaaccactg
    34261 tcgcaatcat tgccgacata cgcgttttga aaatcacaaa cggtctgtcg agatcctgac
    34321 gtgctttaaa gaacgggaaa gccagcgcga ggaacaggta aggaagcgtc atagacacgt
    34381 tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt accgccacca aacgaaacca
    34441 gcaggatgaa gacagtaacc aacccacact gcatccacat ggcgatagac ggcatcccca
    34501 tcgcattcag gcgcgtcatc ggttccggcc acaatgcttt cggcgtcccc tggatgatgg
    34561 ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt ataagcgagg aacatcgaca
    34621 gtccggtaat acgcgcaaac catacgccca gcgacaatga cgcttcaggt gacaaatgca
    34681 gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac ataggtaata ttgccgaggt
    34741 taacggaacc attacttaat acctgctgcc agtttgtgct gacgccccat aaaaatattg
    34801 ccagcgaata accgattgaa ataacaatag cggcaaaaac aatacctttg gcaaagttct
    34861 tttctggatt ttccgtttta tcaaccagac caccaaccgc ttcaattccg ccataggcaa
    34921 aaatggcaaa taccacaaac gatagcattg ccagaccgga ctgataacct gggttcggtg
    34981 atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt taataacaaa atagtaatgc
    35041 ttactaacag caatactaaa ttcagacaca ttactgcaat accgcccacc gcagtaatgc
    35101 gggcaatttt attaatccct tttgaagcaa cgacggtgac cagaatcatc catgccactg
    35161 ccagcagacc aaccacctgc gtaggctcca gtccggcaat atgccagtgc tgggtcatgt
    35221 cgctaccgta gaggaatgtt gagaacggta cccaaacttt cgcggaggta ctcaccatcc
    35281 agatgatata agaggaaaac cacataaacg taccaatgaa ggcaaaacgt ggtccgacac
    35341 tattattcat ccaggaatag ataccgcctt cttctttgcg ataagcagct cccatttcag
    35401 ccatcattaa ggcgaatgga ataaagaata acaatgcaga aaatatataa aaggaaatcg
    35461 cactataacc cattaagtaa taagccgatg ggctattggc aaatccaaat acagaagtaa
    35521 agatcatcaa tatgagtcct atcagactca tcttttttat cgagtgaggc atgaaaccat
    35581 ccttcatttc agcgcgtgta taaaacgcgc ccactgaact gcactgccca acattgagcg
    35641 ggcagcgtaa aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa
    35701 ttgtggcctt tagcggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt
    35761 cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac
    35821 ttcagccagt gtctccgcgc ccagcgccaa catcaccaga cgatcaacac ctaatgccac
    35881 gccggaacag tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg
    35941 ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc
    36001 acgggcatcc gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc
    36061 aaagcgttca gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc
    36121 tggaaagtgg tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt
    36181 aaacagcaat tgtagaagcg tgtcgcggtc ttcttcagta tcggcaacgt tgctcaaatc
    36241 cagtttcgct gccacttccc gcagttgcgt tttgtcggca gagagtgggt caatttccag
    36301 ataacgcaag aaagcttgtt gataagaaag gctttctgct gccggacagt ccagcacctg
    36361 ttgcaagaga tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca
    36421 ctcaagcata gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg
    36481 gcacagctgg aataccggcc cacaaccggc aaccagcagg cgcttcatat ggtattccgg
    36541 gctggtcatt aaccagagat tcatcccctg cgaatgcccg gggccaacga aacgtgtctc
    36601 aaacgggacc aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc
    36661 cagcactcca cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa
    36721 taagttagga atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc
    36781 agtttttgac aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag
    36841 gaataacaca acggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa
    36901 ttaatcatct tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa
    36961 tttcagactt atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt
    37021 cgcagaccgt aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc
    37081 tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg
    37141 ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc
    37201 tcaaaagtat acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc
    37261 gcgcaggatc atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg
    37321 ttgtgtgagc aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa
    37381 ctggaactgt gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc
    37441 ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg
    37501 ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga tccagcgttt cgacgaacat
    37561 ttcgtgctgg atattctagt tgatgatggt catgttcgcg gcctggtagc aatgaacatg
    37621 atggaaggca cgctggtgca gatccgtgct aacgcggtcg ttatggctac cggcggtgca
    37681 ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg
    37741 gcgctaagcc acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt
    37801 ctaccaggtt ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc
    37861 aacaaaaatg gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc
    37921 gagccgaaaa acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg
    37981 cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg gcgatgtggt ttatctcgac
    38041 ctgcgtcacc ttggcgagaa aaaactgcat gaacgtttgc cgttcatctg cgaactggcg
    38101 aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac
    38161 tacaccatgg gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc
    38221 gccgtgggtg aatgttcctc tgttggtctg cacggtgcga accgtctggg ctccaactcg
    38281 ctggcggaac tggtggtctt cggtcgtctg gccggtgaac aagcgacaga gcgtgcagca
    38341 actgccggta atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt
    38401 ctgaaagatc tggttaacca ggatggcggc gaaaactggg ctaagatccg cgacgaaatg
    38461 ggcatggcca tggaagaagg ctgcggtatc taccgtacgc cggaactgat gcagaaaacc
    38521 atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacttcc
    38581 agcgtgttca acaccgacct gctctacacc attgagctgg gccacggtct gaacgttgct
    38641 gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcgca ccagcgtctg
    38701 gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc
    38761 gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca
    38821 gctaaacgtg tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag
    38881 aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc
    38941 ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc
    39001 attactggat gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg
    39061 gtcctgccgt atggcgattt gtggctcctg cggcatgatg gttaacaacg tgccaaaact
    39121 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
    39181 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
    39241 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
    39301 cccggcgcag atggcgaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
    39361 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
    39421 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
    39481 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tactgctccg aagtctgccc
    39541 gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt
    39601 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
    39661 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc
    39721 tgcgcgaagg cacggcagtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
    39781 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
    39841 ttatcgtgat cattaacctg atcactctgg cggcagccct actgcacacc aaaacctggt
    39901 ttgagctggc accaaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
    39961 caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
    40021 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgatgaaccg
    40081 gtattctggg gcctcttcgg ggccggtggt atgtggagcg ccatcattgc gccggtgatg
    40141 atcctactgg ttggtattct gctaccgcta gggctgtttc caggtgatgc gctgagctac
    40201 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
    40261 gttctgccgc tgtggtgtgg tttacaccga atgcaccacg cgatgcacga tctgaaaatc
    40321 cacgtacctg cgggtaaatg ggttttctac ggtctggctg ctatcctgac agttgtcaca
    40381 ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg
    40441 gccgttttgt atggaaacca gaccctatgt tcaaaacgac gctctgcacc ttattaatta
    40501 ccgcctcttg ctccacattt gccgcccctc aacaaatcaa cgatattgtg catcgcacaa
    40561 ttaccccgct tatagagcaa caaaggatcc ccggtatggc ggtagcggta atttatcagg
    40621 gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac
    40681 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
    40741 acgctattgc tcgaggggaa atcaagttaa gcgatcccac aacaaaatac tggcctgaac
    40801 ttaccgctaa acagtggaat gggatcacac tattacatct cgcaacctac accgctggcg
    40861 gcctgccatt gcaggtgccg gatgaggtga aatcctcaag cgacttgctg cgcttctatc
    40921 aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg
    40981 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgaaaa
    41041 ctcgtgtctt ccagccactc aaactcaatc atacgtggat taatgtaccg tccgcagaag
    41101 aaaagaacta cgcctgggga tatcgcgaag gtaaggcagt acatgtttcg ccaggggcgt
    41161 tagatgctga agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa
    41221 gcaatttaaa accccttgat atcaatgaga aaatacttca acaagggata caactggcac
    41281 aatctcgcta ctggcaaacc ggcgatatgt atcagggtct gggctgggaa atgctggact
    41341 ggccggtaaa tcctgacatc atcattaacg gcagtgacaa taaaattgca ctggcagcac
    41401 gccccgtaaa accgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa
    41461 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
    41521 tcgtgatgct ggctaacaaa aactatccca atccagcgag agttaccgcc gcctggcaga
    41581 ttctcaacac tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctccgctt
    41641 ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc
    41701 cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacaccg
    41761 ccgcgtggcg tgaccgtagt gaataatttc gacgctaaac gctatctcgg cacctggtat
    41821 gagattgccc gttttgatca ccgctttgaa cgtggactgg aaaaagtcac cgcaacatac
    41881 agcctgcgtg atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagaa
    41941 atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg
    42001 aaagtgtcat tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa
    42061 taccgccatg cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg
    42121 ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt
    42181 gatgtcagta agtttatttg ggtacagcag cccggtagtt aatgagtgct gagtttcaga
    42241 ccaataatcc ccaatacgat taacgccaga ctcgccagac gcatcgggtt agcggactca
    42301 ccgagcaaca caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca
    42361 taagccgtcc ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc
    42421 atcgccgtca cggtaataac acttggcgtc aaacgactaa agccgtgggt atatttcagg
    42481 ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca ggacatatca
    42541 ggctccggaa aattggggcc gtccccggtg aaaagacgcg tttgcaggtc gtcctgcaaa
    42601 gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctatgta
    42661 actatcgcag aaaaaacaaa cggcacgaca cagctgtcgt accgttattg cttattgctg
    42721 cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc
    42781 acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc
    42841 aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag
    42901 caaattaata atagacaaca tcgccaaaaa tgacggaaat ctgatgattt ttcggaagag
    42961 aagaaaaatt agctggcagc gcgggagatg gagttgccga ggtgtttgat atcttcgccg
    43021 aaacctcgag cggtgttaca gcccgtaagc agcgtgctgg caagcaaaac aagaacgata
    43081 aggcgtttca tcatctctaa cctgaataag tgatggtgca gcctgcaggc cgcaccacaa
    43141 ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg
    43201 ccgatttgta caaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg
    43261 ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatccacgat ttccagttca
    43321 acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct
    43381 gcctgatcca gcaaccattt ggcgttgtcg ccaattgctt ttgcatcagc agacagctgc
    43441 tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg
    43501 ttcatatcga caacatcagc accttcagcg gaatcagtag atttgaaggt tttttctacg
    43561 cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt
    43621 tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca
    43681 gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta
    43741 gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt
    43801 aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga
    43861 cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc
    43921 ccgctcattt atcgatcgca tggagaaagg caatccgaac gatccgcttt tgcgtcaggt
    43981 aattacctcg caagatgagt ttgttgtcgc gcccggattc tccaccgacc cgctggaaga
    44041 acagcacagc gtagtgccag gtttgttgca taaataccac aaccgggcgc ttttgctggt
    44101 caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga
    44161 aaatcagggc aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga
    44221 actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga
    44281 ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg
    44341 tctgccgatt gtgatcccgg cacgcatcac agatgcgctg gttgaacgct tttctcattc
    44401 tacgctgcaa atcttgctgg tgaatcacat taaccatgcc aatgaggtag atgaaacatt
    44461 ccgtcaggcg atggctaagt tgcgccgggt aggcgttact ttgctgaacc agagcgttct
    44521 gttacgtggt gtgaacgata acgcgcaaac gctggcaaac ctgagtaatg cgttgttcga
    44581 tgcgggtgtg atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt
    44641 tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg
    44701 atatctggtg cctaaactgg cgcgagaaat tggcggcgaa cccagcaaaa caccgctgga
    44761 tctccagcta cgccagcagt aactgattaa acagggaagt ttaatctttg cccgttgcat
    44821 aacctttaat tattcatata cattgctgtt attactattt atataaccga caccaataat
    44881 taacctttct tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta
    44941 ttactaccaa gcccatttat ctggcttata aactaaaatt aattatttaa ttgagaaatg
    45001 gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa
    45061 gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt
    45121 gttctttatg tctgccatgg aattgcgcga ccttctgatt gcgctggaaa gtcgtctgca
    45181 tcaaaagcat aaactggatg ctgctgcacg ccttcagtat gaacaagctc gcgataaagt
    45241 cattaagaaa atggcagaaa atatcccaga aattctggtt gatgagctta aaaatgccga
    45301 tattaatcgt cgggttaaca cgctggaact taccgatgat cagggcgaaa atcttacctt
    45361 cgtattaaca ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga
    45421 aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt
    45481 acgtattacc tcactgttag atttcctgcc gttatatgat gtcgactgcc aggatgatgg
    45541 taatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt tcaaccatta
    45601 tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt
    45661 ggtgaaaacg cgtgaagcaa cgccgggtaa agaagtggag gcgatcactc gccgaatgct
    45721 tgatttcagc ccccgactga aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac
    45781 cgtagcggca aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca
    45841 cttacgtgtt caatccacca gcaaaactgc accacaggcg aaataatctt atctgtttta
    45901 ctacgaggct ggaagtatcc agcctctgga tcacatcagt tcgggcactt ataaacctga
    45961 ccgataatct ggctatccgt cgggacaaaa ctggacaaca ttccctgcga cgggctgctg
    46021 atgccataaa tcacgttacc gcccattgca gccgcctggt tgcgcagatc gtttgctgcg
    46081 ccgcgcatag aaccgccttc ttctccgtgt tgcccggaaa gccagttgct ttgcttacct
    46141 gttgcagtac caatcagctg gcactctgca cctggttgct cgtccacaat gcgtacactc
    46201 tgaccggcag cactcaattc gttgctggag ctacaacccg ccatcagtag cgcggcaccg
    46261 acaatccctg ctaagtattt tacgtgcatg ttattcccca taatcaatga gctggacgcc
    46321 actcgcgtcg tccgtgtctg aatcttatac tagaaagatg accaaaagaa aaacccccag
    46381 acatttctgc ccgggggttt gtttatttct gcgaggtgca gggcaattac atcatgccgc
    46441 ccatgccacc catgccgccc ataccgccag cagcgcctaa gtcagctgca tcgtttttcg
    46501 gcaggtcggt aaccatgcac tcggtggtga tcatcaggcc agccacagaa gccgcgtact
    46561 gcagagcaga acgggttact ttggttggat ccaggatacc catatcgatc atgttgccgt
    46621 attcttcagt tgctgcgttg taaccgtagt tgccgtcgcc gcctttaacg gtgttagcaa
    46681 caacagacgg ttcttcgccg cagttcagga cgatctgacg cagcggagct tccattgcac
    46741 gcagtgcaac tttgataccc acgttctggt cttcgttctg accacgcagg tcagccagtt
    46801 tagacgctac gcggatcagc gcaacaccac caccagcaac cacgccttct tctaccgcag
    46861 cacgggtcgc gtgcagggcg tcttcaacgc gtgctttttt ctctttcatt tcaacttcgg
    46921 tagcagcacc aactttgata actgcaacgc cgcctgccag tttcgctacg cgctcctgca
    46981 gtttttcacg gtcgtagtca gaagttgctt cttcaatctg ctgacggatc tgagcaacac
    47041 ggccctggat tgcagcttct tcacccacgc catcgatgat ggtggtggtg tctttgttga
    47101 tcacaacacg tttagcctga cccaggtctt ccagggttgc tttttccagc tccataccga
    47161 tctcttcaga gattacggta ccgccagtca gggttgcgat atcctgcagc atagctttac
    47221 gacgatcgcc gaagcccgga gctttaaccg cagcaacttt cacgatgcca cgcatggtgt
    47281 taacaaccag agttgccagt gcttcgcctt caacatcttc agcgatgatc agcagcggtt
    47341 tacctgcttt ggcaacagct tccagaaccg gcagcatttc gcggatgttg gagattttct
    47401 tgtcagccag caggatgaac gggctttcca gttctactgc gccagtttcc ggcttgttga
    47461 tgaagtaagg agacaagtag ccacggtcga actgcatacc ttcaaccacg tccagttcgt
    47521 cctgcagacc ggtaccgtct tcaacggtga taacgccttc tttaccgact ttgtccatcg
    47581 cttcagcgat cagtttacct acggtttcgt cggagttagc agagatggta ccaacctgag
    47641 caatcgcttt agagtcagag cacggtacgg acagcgcttt cagttcttca actgcagcgg
    47701 taacagcttt gtcgatacca cgtttcaggt ccatcgggtt catgcccgca gcaacagctt
    47761 tcagaccctc agtgatgata gcctgagcca gtacggttgc agtggtggta ccgtcgcctg
    47821 cagcgtcatt cgctttagag gcaacttctt tcaccatctg cgcacccata ttttcgaact
    47881 tgtcttccag ttcgatttca cgagcaacgg aaacaccatc tttggtgatg gtcggtgcac
    47941 cgaaagattt atccagaact acgttacggc ctttaggacc gagggtaact ttcactgcat
    48001 ctgccagtac gtttacgccg cgcagcattt tcacacgagc gtcgttaccg aattttacgt
    48061 ctttagctgc cattatcttt attccttaaa ttcgtatgtt cagtgtcgtg cgcggattac
    48121 gcttcaacaa ttgccagaat gtcgctttcg gacatgatca acacttcttc attgtcgatc
    48181 ttctcagatt tcacaccgta gccatcgttg aaaataacga tgtcgccaac tttcacatcc
    48241 agcggcttca cttcgccatt ttcaaggata cggccattgc cgacagccag cacttcgccg
    48301 cgggtggatt tagccgctgc agagccggtc agaacgatgc cgccagcaga tttagtttca
    48361 acttctttac gcttgacgat cacgcgatca tgcaatggac gaatattcat tgataactct
    48421 cctttgagaa agtccgtatc tgttatgggt gacgccggag cttacgtggt ttcccggctg
    48481 gtgaccagag aaatggggat gaggcttcgc cccttcaagg gggaaaaaga aaaaaaattc
    48541 tgatcagcac aaaatcgggt gaaaaccctg attcacctca catttcatcg ctatttcttc
    48601 atcaccgtca taagcaaatc tgattgtgct accatcgaaa atctacgcag ttgcccaaaa
    48661 tttgggcgca atcggacatc aacccatgag tggactcaaa caagaactgg ggctggccca
    48721 gggcatcggc ctgctatcga cgtcattatt aggcactggc gtgtttgccg ttcctgcgtt
    48781 agctgcgcta gtagcaggca ataacagcct gtgggcgtgg cccgttttga ttatcttagt
    48841 gttcccgatt gcgattgtgt ttgcgattct gggtcgccac tatcccagcg caggcggcgt
    48901 cgcgcacttc gtcggtatgg cgtttggttc gcggcttgag cgagtcaccg gctggttgtt
    48961 tttatcggtc attcccgtgg gtttgcctgc cgcgctacaa attgctgccg gattcggtca
    49021 ggcaatgttt ggctggcata gctggcaact gttgttggca gaactcggta cgctggcgct
    49081 ggtgtggtat atcggtactc gcggtgccag ttccagtgct aatctacaaa cagttattgc
    49141 cggacttatc gtcgcactga ttgtcgctat ctggtgggcg ggcgatatca aacctgcgaa
    49201 tatccccttc cctgcgccag gaaatatcga acttaccggg ttatttgctg cgttatcagt
    49261 gatgttctgg tgttttgtcg gtctggaagc atttgcccat cttgcctcgg aatttaaaaa
    49321 tccagagcgt gattttcctc gtgctttgat gattggcctg ctgctggcag gattagtcta
    49381 ttggggctgt acggtagtcg tcttacactt cgacgcctat ggtgaacaaa tggcggcggc
    49441 agcatcgctt cccaaaattg tagtgcagtt gttcggtgta ggagcgttat ggattgcctg
    49501 cgtaattggc tatctggcct gctttgccag tctcaacatt tatatacaga gcttcgcccg
    49561 cctggtctgg tcgcaggcgc aacataatcc tgaccactac ctggcacgcc tctcttctcg
    49621 ccatatcccg aataatgccc tcaatgcggt gctcggctgc tgcgtggtaa gcacgttggt
    49681 gattcatgct ttagagatca atctggacgc tcttattatt tatgccaatg gcatctttat
    49741 tatgatttat ctgttatgca tgctggcagg ctgtaaatta ttgcaaggac gttatcgact
    49801 actggcggtg gttggcgggc tattatgcgt tctgttactg gcaatggtcg gctggaaaag
    49861 tctctacgcg ctgatcatgc tggcggggtt atggctgttt ctgccaaaac gaaaaacgcc
    49921 ggaaaatggc ataaccacat aatccggcgt ttcgacatta atcctggcga tcgtctttat
    49981 gatcaaggcg gtcgcggtca tcatcctttc gctggtactc accatcaaaa gtattaccgc
    50041 caccggtccc ggcgctaaaa ccgccgcctg gcatgcgaga aaagcgcaaa tgcggcatca
    50101 acttcactgt cagatgcttt tgcaccggcg gcaataaaag cagaagaccg aggaagtcgg
    50161 tgaaaaagcc cggtaataaa agcagcaaac cggcaatgat cagcgaaaca cttttaatca
    50221 tctccgccgc tgggttttca cccgccgcca ttttttgctg catcagcacg aaattcttaa
    50281 agccctggtt acgtaccagt gacataccga taactgacgt gaatataacc agcacgaggg
    50341 tcagcaatac ccccaataca tgagcaacct gaataaagat tgaaatctca atatagacat
    50401 aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat agcagccggt
    50461 taaacccggc tacctgaatg ggttgcgaat cgcgtttagc ttatattgtg gtcattagca
    50521 aaatttcaag atgtttgcgc aactattttt ggtagtaatc ccaaagcggt gatccatttc
    50581 acaaattaat aattaagggg taaaaaccga cacttaaagt gatccaggtt acggtagaaa
    50641 tcctcaagca gcatatgatc tcgggtattc ggtcgatgca ggggataatc gtcggtcgaa
    50701 aaacattcga aaccacatat attctgtgtg tttaaagcaa atcattggca gcttgaaaaa
    50761 gaaggttcac atgtcaaaca acattcgtat cgaagaagat ctgttgggta ccagggaagt
    50821 tccagctgat gcctactatg gtgttcacac tctgagagcg attgaaaact tctatatcag
    50881 caacaacaaa atcagtgata ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc
    50941 cgcagctatg gcaaacaaag agctgcaaac cattcctaaa agtgtagcga atgccatcat
    51001 tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt
    51061 ctaccagggc ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc tggccaatat
    51121 cggtctggaa ctgatgggtc accagaaagg tgaatatcag tacctgaacc cgaacgacca
    51181 tgttaacaaa tgtcagtcta ctaacgacgc ctacccgacc ggtttccgta tcgcagttta
    51241 ctcttccctg attaaactgg tagatgcgat taaccaactg cgtgaaggct ttgaacgtaa
    51301 agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc
    51361 gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa
    51421 catccaacgt accgctgaac tgctgctgga agttaacctt ggcgcaacag caatcggtac
    51481 tggtctgaac acgccgaaag agtactctcc gctggcagtg aaaaaactgg ctgaagtcac
    51541 tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg acctccgact gcggcgctta
    51601 tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga
    51661 cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac gagatcaacc tgccggaact
    51721 gcaggcaggc tcttccatca tgccagctaa agtaaacccg gttgttccgg aagtggttaa
    51781 ccaggtatgc ttcaaagtca tcggtaacga caccactgtt accatggcag cagaagcagg
    51841 tcagctgcag ttgaacgtta tggagccagt cattggccag gccatgttcg aatccgttca
    51901 cattctgacc aacgcttgct acaacctgct ggaaaaatgc attaacggca tcactgctaa
    51961 caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt atcgttactt acctgaaccc
    52021 gttcatcggt caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag
    52081 tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa gcggaacttg acgatatttt
    52141 ctccgtacag aatctgatgc acccggctta caaagcaaaa cgctatactg atgaaagcga
    52201 acagtaatcg tacagggtag tacaaataaa gaaggcacgt cagatggcgt gccttttttc
    52261 ttgtgagcag taacttaaaa ataacaattt aatatcaact tgttaaaaaa caaggaaggc
    52321 taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg gcgccagatt
    52381 ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg ccgctattgg
    52441 cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg ttatcgccgc
    52501 tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga cagaaaagct
    52561 gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct atttcctgac
    52621 tatctttgct ggtactggca acatctctct ggcgacactg ccagttatcg ctgaagttgc
    52681 gaaggaacaa ggcgttaaac cttgccgtcc gctgtctact gcagtggtat ccgcgcagat
    52741 tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg tgatggaagg
    52801 tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc tgctggcggt
    52861 tctggtgatg tccttcctgg tcactatgct gttcaactcc aaactctctg acgatccgat
    52921 ttatcgcaag cgtctggaag agggcctggt tgaactgcgc ggtgaaaagc agattgaaat
    52981 caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg gcgtggttat
    53041 ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccactga tgaacaccac
    53101 caacgcaatc ctgatcatca tgctcagcgt tgcaactctg accaccgtta tctgtaaagt
    53161 cgataccgac aacattctca actccagcac cttcaaagca ggtatgagcg cctgtatttg
    53221 tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg actggatcaa
    53281 agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca tcttcttctt
    53341 tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc cgatggctct
    53401 ggcactgaac gtttctccgc tgaccgctgt tgcttctttt gctgcggtgt ctggtctgtt
    53461 cattctgccg acctacccga cgctggttgc tgcggtacag atggatgaca cgggtactac
    53521 ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc tgggtgttgc
    53581 cctggccgtt tgcttcggct tcgtgctggg tagcttcatg ctgtaatgac ccatcgcggg
    53641 gcgttcacgc cccgctttct ttcccgccga ctaacatcct ttccccgtcc gttgtatagt
    53701 gacctctctc ttgcagttcc atctgttctt gcgaggtgtt tatgcttgat gaaaaaagtt
    53761 cgaataccac gtctgtcgtg gtgctatgta cggcaccgga tgaagcgaca gcccaggatt
    53821 tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc cccggcgcta
    53881 cctctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag atgattttaa
    53941 aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat catccatatc
    54001 aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac ctctcatggc
    54061 tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg ccggattatt
    54121 cgacgcgccg ggacgttcac aatttgtccc cgcggatcaa gcctttgctt ttgattttca
    54181 gcaaaaccaa catgacctta atctgacctg gcagatcaaa gacggttact acctctaccg
    54241 taaacagatc cgcattacgc cggaacacgc gaaaattgct gacgtgcagc tgccgcaagg
    54301 cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc ggctgacgct
    54361 tcccgtaacc atcaaccagg caagtgcggg agcaacgtta actgtcacct accagggctg
    54421 tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa gcgaagtggt
    54481 cgccaacaac gaagcgtcac agcctgtgtc tgttccgcag caagagcagc ccaccgcgca
    54541 attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct ttacgccatg
    54601 cgtgctgcca atgtacccac tgatttctgg catcgtgctg ggcggtaaac agcggctttc
    54661 cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcgc tgacttacac
    54721 ggcgctgggt ctggtggttg ccgccgcagg gttacagttc caggcggcgc tacagcaccc
    54781 atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa tgtttggctt
    54841 gtttactctg caactcccct cttcgctgca aacacgtctc acgctgatga gcaatcgcca
    54901 acagggcggc tcacctggcg gtgtgtttat tatgggggcg attgccggac tgatctgttc
    54961 accatgcacc actgcaccgc ttagcgcgat tctgctgtat atcgcccaaa gcgggaacat
    55021 gtggctgggc ggcggcacgc tttatcttta tgcgctgggc atgggcctgc cgctgatgct
    55081 aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc ccgtggatgg aacaagtcaa
    55141 aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc gagtgattgg
    55201 tgatatatgg ggattacgct tgtggtcggc gcttggtgtc gcattctttg gctgggcctt
    55261 tatcaccagc ctacaggcca aacgcggctg gatgcgcgtg gtgcaaataa tcctgctggc
    55321 agcggcattg gttagcgtac gcccacttca ggattgggca tttggtgcaa cacataccgc
    55381 gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt taaatcaggc
    55441 gctcgttgaa gccaaaggca aaccagtgat gttagatctc tatgccgact ggtgcgtcgc
    55501 ctgtaaagag tttgagaaat acaccttcag cgactcgcag gtgcaaaaag cgttagcaga
    55561 tacggtctta cttcaggcca acgtcactgc caacgacgca caagatgtgg cgctgttaaa
    55621 gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag gccaggagca
    55681 tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac atttgcgcga
    55741 tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg tgcaacgtga
    55801 agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca acaccacgct
    55861 ggagatggtt gctgaacgtg tggattatcc actggacgag ctacgccgct tctggccaga
    55921 caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag atgtctggcg
    55981 tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagttgctgg cacgttatca
    56041 ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctgttta tcgctgcctg
    56101 tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc aaaaaagcgc
    56161 ggcctacgat ttcacccacg aactgttaac cacgctggaa gttgacgatc cggcgatggt
    56221 agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg tgaatcgtag
    56281 ccaggcggat gtcgacaccg cacatcggct ggcggaagat atcctgcgct tcgcccgctg
    56341 ccgtcagggt ggtgcactga cctgacagaa acacagaaaa gaagcgattt gccgcaatct
    56401 taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat tacggtttaa
    56461 tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc
    56521 ttggttcgat tccgagtccg ggcaccaaat tcatataaac ggacctccac ggaggtccat
    56581 ttttcgtttc aggacgccac gatttaagcg tcctgccgcc aaatcaattc taccgaactc
    56641 aaccagattc tccccacatc accagcaatt tgcgggcata tcccaattcg ggaaaatttg
    56701 tttctgagct atagcgctga ctgacgtgaa atgtcgtgcg gccccgtgat gctgttgaac
    56761 gtcaaatgac gtcatcagga gcgtaatgca tccataaagc acaacatcgg gcagaacgcc
    56821 aactgatgag attttctgaa tgagaacaaa gagaaatgta tcagtccgtt tgctcatgca
    56881 aagactaaca atccattaaa atagtaagcg ctccggacaa tttttcatgg attattttct
    56941 gaacattttt ctttggcaaa gatgatgaat tttgatggta aggaaaatta cttctggttc
    57001 tc
//
LOCUS       DAPQZD010000011        54062 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPQZD010000011 DAPQZD010000000
VERSION     DAPQZD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.011"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            89..502
                     /locus_tag="RO454_000685"
     CDS             89..502
                     /locus_tag="RO454_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDY0932563.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(547..864)
                     /gene="hspQ"
                     /locus_tag="RO454_000686"
     CDS             complement(547..864)
                     /gene="hspQ"
                     /locus_tag="RO454_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDY0932564.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(922..2112)
                     /gene="rlmI"
                     /locus_tag="RO454_000687"
     CDS             complement(922..2112)
                     /gene="rlmI"
                     /locus_tag="RO454_000687"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDY0932565.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2207..2485
                     /gene="yccX"
                     /locus_tag="RO454_000688"
     CDS             2207..2485
                     /gene="yccX"
                     /locus_tag="RO454_000688"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDY0932566.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2482..2811)
                     /gene="tusE"
                     /locus_tag="RO454_000689"
     CDS             complement(2482..2811)
                     /gene="tusE"
                     /locus_tag="RO454_000689"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDY0932567.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2903..3562)
                     /gene="yccA"
                     /locus_tag="RO454_000690"
     CDS             complement(2903..3562)
                     /gene="yccA"
                     /locus_tag="RO454_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDY0932568.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3771..3858)
                     /locus_tag="RO454_000691"
     tRNA            complement(3771..3858)
                     /locus_tag="RO454_000691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3822..3824),aa:Ser,seq:tga)
     gene            4286..5404
                     /gene="hyaA"
                     /locus_tag="RO454_000692"
     CDS             4286..5404
                     /gene="hyaA"
                     /locus_tag="RO454_000692"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDY0932569.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5401..7194
                     /gene="hyaB"
                     /locus_tag="RO454_000693"
     CDS             5401..7194
                     /gene="hyaB"
                     /locus_tag="RO454_000693"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDY0932570.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQLIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7213..7920
                     /gene="hyaC"
                     /locus_tag="RO454_000694"
     CDS             7213..7920
                     /gene="hyaC"
                     /locus_tag="RO454_000694"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDY0932571.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7917..8504
                     /locus_tag="RO454_000695"
     CDS             7917..8504
                     /locus_tag="RO454_000695"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDY0932572.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQG"
     gene            8501..8899
                     /gene="hyaE"
                     /locus_tag="RO454_000696"
     CDS             8501..8899
                     /gene="hyaE"
                     /locus_tag="RO454_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDY0932573.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8896..9753
                     /locus_tag="RO454_000697"
     CDS             8896..9753
                     /locus_tag="RO454_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDY0932574.1"
                     /translation="MSETFFHLLGPGTQLNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            9887..11431
                     /gene="appC"
                     /locus_tag="RO454_000698"
     CDS             9887..11431
                     /gene="appC"
                     /locus_tag="RO454_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDY0932575.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11443..12579
                     /gene="appB"
                     /locus_tag="RO454_000699"
     CDS             11443..12579
                     /gene="appB"
                     /locus_tag="RO454_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDY0932576.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12592..12684
                     /gene="cbdX"
                     /locus_tag="RO454_000700"
     CDS             12592..12684
                     /gene="cbdX"
                     /locus_tag="RO454_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDY0932577.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12764..14074
                     /gene="appA"
                     /locus_tag="RO454_000701"
     CDS             12764..14074
                     /gene="appA"
                     /locus_tag="RO454_000701"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDY0932578.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEVRIPACALHQDK"
     gene            complement(14062..14274)
                     /gene="cspH"
                     /locus_tag="RO454_000702"
     CDS             complement(14062..14274)
                     /gene="cspH"
                     /locus_tag="RO454_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDY0932579.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14560..14772
                     /gene="cspG"
                     /locus_tag="RO454_000703"
     CDS             14560..14772
                     /gene="cspG"
                     /locus_tag="RO454_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDY0932580.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14783..14971
                     /locus_tag="RO454_000704"
     CDS             14783..14971
                     /locus_tag="RO454_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDY0932581.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            14946..15176
                     /gene="ymcE"
                     /locus_tag="RO454_000705"
     CDS             14946..15176
                     /gene="ymcE"
                     /locus_tag="RO454_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDY0932582.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            15166..15339
                     /locus_tag="RO454_000706"
     CDS             15166..15339
                     /locus_tag="RO454_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDY0932583.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(15388..16461)
                     /locus_tag="RO454_000707"
     CDS             complement(15388..16461)
                     /locus_tag="RO454_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDY0932584.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLAFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(16544..19276)
                     /gene="torS"
                     /locus_tag="RO454_000708"
     CDS             complement(16544..19276)
                     /gene="torS"
                     /locus_tag="RO454_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDY0932585.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRIDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRVPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            19359..20387
                     /gene="torT"
                     /locus_tag="RO454_000709"
     CDS             19359..20387
                     /gene="torT"
                     /locus_tag="RO454_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDY0932586.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(20360..21052)
                     /gene="torR"
                     /locus_tag="RO454_000710"
     CDS             complement(20360..21052)
                     /gene="torR"
                     /locus_tag="RO454_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDY0932587.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            21182..22354
                     /gene="torC"
                     /locus_tag="RO454_000711"
     CDS             21182..22354
                     /gene="torC"
                     /locus_tag="RO454_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDY0932588.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            22354..24900
                     /gene="torA"
                     /locus_tag="RO454_000712"
     CDS             22354..24900
                     /gene="torA"
                     /locus_tag="RO454_000712"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDY0932589.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            24897..25496
                     /gene="torD"
                     /locus_tag="RO454_000713"
     CDS             24897..25496
                     /gene="torD"
                     /locus_tag="RO454_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDY0932590.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(25751..26056)
                     /gene="cbpM"
                     /locus_tag="RO454_000714"
     CDS             complement(25751..26056)
                     /gene="cbpM"
                     /locus_tag="RO454_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDY0932591.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(26056..26976)
                     /gene="cbpA"
                     /locus_tag="RO454_000715"
     CDS             complement(26056..26976)
                     /gene="cbpA"
                     /locus_tag="RO454_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDY0932592.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            27511..28752
                     /gene="agp"
                     /locus_tag="RO454_000716"
     CDS             27511..28752
                     /gene="agp"
                     /locus_tag="RO454_000716"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDY0932593.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(28790..29017)
                     /locus_tag="RO454_000717"
     CDS             complement(28790..29017)
                     /locus_tag="RO454_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDY0932594.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(29038..29634)
                     /gene="wrbA"
                     /locus_tag="RO454_000718"
     CDS             complement(29038..29634)
                     /gene="wrbA"
                     /locus_tag="RO454_000718"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDY0932595.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30007..30180
                     /locus_tag="RO454_000719"
     CDS             30007..30180
                     /locus_tag="RO454_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDY0932596.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(30263..31591)
                     /gene="rutG"
                     /locus_tag="RO454_000720"
     CDS             complement(30263..31591)
                     /gene="rutG"
                     /locus_tag="RO454_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDY0932597.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(31612..32106)
                     /gene="rutF"
                     /locus_tag="RO454_000721"
     CDS             complement(31612..32106)
                     /gene="rutF"
                     /locus_tag="RO454_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDY0932598.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(32117..32707)
                     /locus_tag="RO454_000722"
     CDS             complement(32117..32707)
                     /locus_tag="RO454_000722"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDY0932599.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(32717..33517)
                     /gene="rutD"
                     /locus_tag="RO454_000723"
     CDS             complement(32717..33517)
                     /gene="rutD"
                     /locus_tag="RO454_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDY0932600.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(33525..33911)
                     /gene="rutC"
                     /locus_tag="RO454_000724"
     CDS             complement(33525..33911)
                     /gene="rutC"
                     /locus_tag="RO454_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDY0932601.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(33923..34615)
                     /gene="rutB"
                     /locus_tag="RO454_000725"
     CDS             complement(33923..34615)
                     /gene="rutB"
                     /locus_tag="RO454_000725"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDY0932602.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(34615..35706)
                     /gene="rutA"
                     /locus_tag="RO454_000726"
     CDS             complement(34615..35706)
                     /gene="rutA"
                     /locus_tag="RO454_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDY0932603.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            35994..36632
                     /gene="rutR"
                     /locus_tag="RO454_000727"
     CDS             35994..36632
                     /gene="rutR"
                     /locus_tag="RO454_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDY0932604.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(36672..40634)
                     /gene="putA"
                     /locus_tag="RO454_000728"
     CDS             complement(36672..40634)
                     /gene="putA"
                     /locus_tag="RO454_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDY0932605.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            40689..40898
                     /locus_tag="RO454_000729"
     CDS             40689..40898
                     /locus_tag="RO454_000729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932606.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            41057..42565
                     /gene="putP"
                     /locus_tag="RO454_000730"
     CDS             41057..42565
                     /gene="putP"
                     /locus_tag="RO454_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDY0932607.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            42785..43615
                     /locus_tag="RO454_000731"
     CDS             42785..43615
                     /locus_tag="RO454_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDY0932608.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            43673..44800
                     /gene="efeO"
                     /locus_tag="RO454_000732"
     CDS             43673..44800
                     /gene="efeO"
                     /locus_tag="RO454_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDY0932609.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            44806..46077
                     /gene="efeB"
                     /locus_tag="RO454_000733"
     CDS             44806..46077
                     /gene="efeB"
                     /locus_tag="RO454_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDY0932610.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            46704..47492
                     /gene="phoH"
                     /locus_tag="RO454_000734"
     CDS             46704..47492
                     /gene="phoH"
                     /locus_tag="RO454_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDY0932611.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(47542..47955)
                     /gene="pgaD"
                     /locus_tag="RO454_000735"
     CDS             complement(47542..47955)
                     /gene="pgaD"
                     /locus_tag="RO454_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDY0932612.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(47957..49282)
                     /gene="pgaC"
                     /locus_tag="RO454_000736"
     CDS             complement(47957..49282)
                     /gene="pgaC"
                     /locus_tag="RO454_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDY0932613.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(49275..51293)
                     /gene="pgaB"
                     /locus_tag="RO454_000737"
     CDS             complement(49275..51293)
                     /gene="pgaB"
                     /locus_tag="RO454_000737"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDY0932614.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(51302..53725)
                     /gene="pgaA"
                     /locus_tag="RO454_000738"
     CDS             complement(51302..53725)
                     /gene="pgaA"
                     /locus_tag="RO454_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDY0932615.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
BASE COUNT        13006 a        13760 c        14266 g        13030 t
ORIGIN      
        1 cccgcttttg ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta
       61 cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac
      121 gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg
      181 cgtgatgaaa tatctactcg accaggggta tcacgtcatt cctgtttcgc caaaagttgc
      241 cggcaaaaca ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaagt
      301 cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcgc aggaagctat
      361 cgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt
      421 actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc
      481 tcgcctgggc ctggcaaaat aaaaaatacc cggaaggcga aaaccttccg ggtatgttta
      541 tgagaattag ttacgcagac gcggcgcctg gagttgtttg cggatggtct gcgccagctc
      601 atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc
      661 caggtaggta tgaaccggta ggccgttatc atcctccatc accacgtgat accacggagc
      721 ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgagtaaac
      781 cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc
      841 gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag
      901 ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc
      961 ttccgggtag gtagcgatca ccggatgatc ggcggcctga cggaactgct ctataaattg
     1021 tacatcacga ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt
     1081 catcagaccg gaacaggaga aagtcaggag aacaccgcct tcattcagca actgaatcgc
     1141 cagcatgttg atgtctttat agccacggca cgcgcccatc aactggcttt tattctcaac
     1201 aaacttcggc gggtccatca cgataacatc aaatttttca ccgcgatcgc gataagtacg
     1261 cagcaattta aagacatcat cacggacaaa ctctgccttg ctcagatcca gtttgttcag
     1321 ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg
     1381 gctgcaaccg cccatcaatg ccgatacggc gaaaccaccg gtataggaga aacagttaag
     1441 tacgcgttta ttttcgacgt agcggcgggt agccaggcgg ctgtcacgct ggtccaggta
     1501 gtagcccgtt ttgtgcccgt gctgaatatc caccagcagt ttcatgccgt gttcttcaat
     1561 cggcagcagg gcaggcggca actcgccggt gatggggccc tgagtcagct ccatcccttc
     1621 ttttttacgt accgcgacgt cgctgcgatc gtaaatggca cattccgggt acaacgtttg
     1681 cagggcgcta attaatgccg cgcgctgata ctctgcgcca gcactcagca attgcagcac
     1741 cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt caccggcgat
     1801 taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg
     1861 ctgcaaacgg cgggtaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac
     1921 gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg
     1981 atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc
     2041 ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa
     2101 acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt
     2161 tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg
     2221 cataattgcc tgggtttacg ggcgagttca gggcgtagga ttccgctaca ccacgcagta
     2281 cgaagcgaaa aaactgggac taaccgggta cgccaaaaat cttgacgatg gcagcgtgga
     2341 agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta atgcagtggt taaaaagtgg
     2401 cggcccgcgt tctgcgcgtg tggaacgggt gctcagcgag ccgcatcatc cctccgggga
     2461 gttaacggat tttcgtatcc gttaaatgca ttttaccggt ttaggcaggc cagcaatttt
     2521 ggtggcttgc tttgccggac ctttcgggaa cagtcggtac agatagcggc tattgccttt
     2581 ctcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg gagaagtatt
     2641 gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttctg gcgacagagt
     2701 aatcccttcg ttttctgcaa tcaccaccgc cagcggctca ctccactggc tgctttcttt
     2761 gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca taattcttta
     2821 ctaccttaat cagactggcg gcagtgtaac aaaaaacaaa gccccgcata aagcgaggct
     2881 cggtaagtaa ccctgggcga gattaatcgc ggctggcgaa gcccagaatg ctcagcaggc
     2941 tgacgaagat gttgtacagc gaaacataca ggctaaccgt ggcacgaata tagttcgttt
     3001 caccgccatg aatgatgttg ctggtttcaa acaagatagc gccagaggag atcagaatga
     3061 agaccgcgct gatcgccaga tgcagagcag gcagttgcag gaagatatta gcaaccatac
     3121 caatcagcac caccacaata cccgccatca gcataccgcc gaggaaagac atatctttgc
     3181 gggtggtcag cacatacgca gagcagcaga agaacactaa cgccgttccg cccagtgcca
     3241 tagcgattac gtcacccatt ccggcagaca gataggtgtt cagaattggt ccgaggatat
     3301 aacccagaaa accggtaaag gcgaatgcgg agataatccc ggtcggctta ttcgccgttt
     3361 tgtaggtcag gaacatcaaa ccatacatac ccaccagcgt cagaatcaga cccggagatg
     3421 gcagcatcag caccgtactg gcagttgcgg taatcgccga aaaggccagc gtcaggctca
     3481 gcagaaaata ggtattacgc agcaccttat gggtgctaag cagcgatgta cggtcatgtg
     3541 aagaactaac tatacgatcc attgagtcac tctctatgac agatgtaatt aattaagcag
     3601 cataatgata atgcgtaagg gcacccagaa gttttaccca tctttacgca tttgatctgg
     3661 aacaggttta acagcggatt atcaggtcat taggcagtga tataacgccc tgagaatttc
     3721 gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc
     3781 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa
     3841 ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac
     3901 ccctaatttg tttgtttgcc tgaaaaatag ccaaaagtgc attgatagcg tgaaacaaca
     3961 gcagattgat catttcatca ccatgaattc cttctctatt tactcgttta gcaaccggct
     4021 aaacatcccc accacccggc aaaaagaaaa ataggtccat ttttatcgct aaaagataaa
     4081 tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa caatactttc
     4141 tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt cagcatctca cgtgaagcga
     4201 tggtttgcct tgctacaggg acgtcgcttg ccgaccatgt gcgcccggtg ttctgccggt
     4261 gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa acattttacc aggccatgcg
     4321 gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg ccacgtcgct
     4381 gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaata aaccgcgcat
     4441 tccggtggta tggatccacg gcctggaatg cacctgctgt accgaatctt ttatccgctc
     4501 cgctcaccca ctggcgaagg acgtcatcct ttccctgatt tccctcgatt acgacgatac
     4561 tttgatggct gccgccggaa cccaggcgga agaagtcttt gaagacatca tcgcgcaata
     4621 caatggcaaa tatatcctcg cagtagaagg taatccgccg ctgggcgagc aggggatgtt
     4681 ctgtatcagc agcggacgac cgtttattga gaaactcaag cgtgccgccg ctggagccag
     4741 tgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gttcaggccg cgcgacctaa
     4801 tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacccatta tcaaagtacc
     4861 tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg tgacgtttga
     4921 tcgtctgccg gatgtcgaca gaatgggccg tccactgatg ttttatggtc agcgtatcca
     4981 cgataaatgc tatcgccgcg ctcacttcga cgccggagag ttcgtccaga gttgggatga
     5041 tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc ctaccaccta
     5101 taacgcctgt tcctccacac gctggaacga tggcgtttcc ttcccgatcc agtcaggtca
     5161 cggctgcctg ggttgtgcgg aaaatggttt ctgggatcgc ggttcgttct acagccgcgt
     5221 ggtcgatatt ccacaaatgg gtacccattc caccgccgac accgtcggtt taaccgcgct
     5281 tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg accagcgcag
     5341 acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg ataaacaggc
     5401 atgagcactc agtacgaaac tcagggatac accatcaaca atgccggacg ccgcctggtg
     5461 gtcgacccga ttacgcgcat tgaaggccac atgcgctgcg aagtgaatat taacgatcag
     5521 aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga gatcattctg
     5581 caagggcgtg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg cgtctgtact
     5641 ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ccatcggtat taaagtgccg
     5701 gacaacgcca atatcatccg caacattatg ctggcaacgc tctggtgcca cgatcatctg
     5761 gtgcacttct atcagcttgc cgggatggat tggatcgatg tgttagatgc gctgaaagcc
     5821 gacccgcgga aaacctccga actggcgcaa agcctctcct catggccgaa atcatcccct
     5881 ggctatttct tcgacgtaca aaaccgcctg aagaaattcg ttgaaggcgg ccagttgggg
     5941 atcttccgca atggctactg ggggcacccg cagtacaaac tgccgccaga agccaacctg
     6001 atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt caaaatccac
     6061 gcggtctttg gcggtaaaaa cccgcatcca aactggattg tcggtgggat gccttgcgcc
     6121 attaacattg acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct gaacctggtg
     6181 cagttgatta tcacccgcac ggcggatttc attaacaacg tgatgatccc cgacgcctta
     6241 gccatcggtc agttcaacaa accgtggagc gaaatcggca cggggctttc tgataaatgc
     6301 gtcctcagtt acggcgcgtt ccctgatatc gccaacgact ttggcgagaa aagtctgctg
     6361 atgcctggcg gcgcggtgat taacggcgac ttcaacaatg tactgccagt ggatttagtt
     6421 gatccgcagc aggtgcagga gtttgttgac catgcctggt atcgttatcc caacgatcag
     6481 gttgggcgtc atccgttcga tggcattacc gacccgtggt acaaccccgg cgatgtcaaa
     6541 ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg gatcaaagcg
     6601 ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cacgcacgtt aatcgcttat
     6661 cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgta tgatgtcggc gttgaaactg
     6721 ccgctttccg gcatccagtc aacgttgggc cgcattttgt gtcgcgcgca cgaagcgcag
     6781 tgggccgcag gtaagttgca gtatttcttc gataaactga tgaccaacct gaaaaacggc
     6841 aatcttgcca ccgcttccac ggaaaaatgg gaaccagcaa cctggccgac agagtgccgt
     6901 ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc actgggctgc cattcgcgat
     6961 ggcaagattg atctctatca gtgcgtggtg ccgaccacct ggaacgccag cccgcgcgat
     7021 cccaaagggc agattggcgc ttatgaagcg gcgctgatga acaccaaaat ggcgatcccc
     7081 gagcaaccgc tggagatcct gcgtactctg cacagctttg acccgtgcct cgcctgttca
     7141 acacacgtgc tgggcgacga cggtagcgag cttatctccg tgcaggtgcg ttaacagcga
     7201 aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gtcactatgt ctttgaagca
     7261 cccgtgcgga tatggcactg gctgacagtg ttatgcatgg cggtgttgat ggtcaccggt
     7321 tactttatcg gcaagccgct accttccgtc agcggcgagg cgacatatct gttctatatg
     7381 ggctacatca ggttaattca cttcagcgcc gggatgattt ttaccgtggt tttgctgatg
     7441 cggatctact gggcttttgt tggcaatcgt tactcccgcg agctgtttat cgtgccggta
     7501 tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct gtttctggca
     7561 aaacgtccga gtgccgatat aggccataac cccatcgccc aggcggcgat gttcggctat
     7621 ttcctgatgt cagtttttat gatcatcacc ggctttgcgc tgtacagcga acacagccag
     7681 tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggac gggcggcaac
     7741 tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc atttgtgatc
     7801 ggccatgtct acatggcgct gcgtgaagac atcatgtccg acgacacggt gatctccact
     7861 atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga gcgttcatga
     7921 gcgagcaacg cgtggtggta atggggctgg gcaacctgct gtgggccgat gaaggcttcg
     7981 gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg gcccgaggat gtggagattg
     8041 tcgatggcgg cacccaagga ctgaacttgc tggggtatgt cgaaagcgcc agtcatctgt
     8101 tgattctcga tgccattgat tacgggctgg aacccggaac gctacgaacg tatgccggag
     8161 aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcaccagaac agtttctccg
     8221 aagtgctggc gctggcagat atccgcggac atctgccagc acatattgcc ctcgtcggtc
     8281 tgcaacccgc aatgctcgac gactacggcg gcagcctgag cgaactggcg cgggagcaac
     8341 tgcccgccgc ggaacaggcg gcgctggcgc agctggctgc gtggggaatt gtgccgcaac
     8401 cggctaatga atcgcgctgt ctcaattatg actgtctgtc gatggaaaat tacgaaggca
     8461 ttcgtttgcg ccagtaccgg atgacactgg aggagcaggg atgagcaacg acacgccatt
     8521 tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccgatcagtg aatcccgtct
     8581 tgacgactgg cttacgcaag cgccagacgg cgtggtgtta ttaagcagcg atccgaaacg
     8641 cacgccagag gtcagcgata atccggtaat gattggcgaa ttattgcgcg agtttcccga
     8701 ttatacatgg caggtagcga ttgctgacct tgagcagagc gaagccatcg gcgatcgctt
     8761 tggcgtcttt cgctttcccg ccacattagt atttacctgc ggaaactatc gcggcgtgct
     8821 gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcggacttg tcgaaccgca
     8881 gcaggagcgt gcctcatgag cgaaactttt ttccatctgc tggggccagg aacgcaactg
     8941 aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg
     9001 agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct gttaaacgag
     9061 ttacaacatc aactaagcga acgccagccg ccgttgggcg aggtactggc agtcgatctg
     9121 ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga aggggaagtg
     9181 tcagtgcgca ttcagcaggc tgatggtagt gaaagtgaaa ttcaggaggc gatcttctgc
     9241 ggattatggc gggtacgcag acgtcacggc gaacagttgc tggaagacaa actggaggct
     9301 ggctgcgcac cgctggcgtt gtggcaggcg gcaacgcaaa acgtcttgcc gacagattcg
     9361 ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggccca tgaattactg
     9421 gcacatgtac gtaaccccga cgcacagccg cacagcatta atctgacgca attacccatc
     9481 agcgaagctg accggctttt tctctcacgt ctctgtgggc cgggaaatat tcagattcgt
     9541 accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt ctggcattta
     9601 cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt atgaaatctg cccaataccg
     9661 gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct tagcgaggta
     9721 tgccagtggc tggcggaagg tgcaccgacg taaaaagacg gtaagtatcg ctttcaggct
     9781 tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa agagggagat
     9841 ctaccattaa cgggttattt ttctctcttc gcctacagga gtgcgcatgt gggatgtcat
     9901 tgatttatcg cgctggcagt ttgctctgac cgcgctgtat cactttttat ttgtacccct
     9961 taccctgggg ctgatttttt tgctggctat catggaaacc atttacgtgg tcaccggcaa
    10021 aacaatctac cgcgatatga cgcgcttctg gggtaagctc ttcggtatca actttgctct
    10081 tggcgtggct accggcctga cgatggagtt tcagtttggt actaactggt cattctattc
    10141 caactatgtg ggcgatattt ttggcgcacc actggcgatg gaagcattaa tggccttctt
    10201 cctcgaatcc acctttgtcg ggctgttctt cttcggctgg caacggctga ataaatacca
    10261 gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat ctctctgcgt tgtggatatt
    10321 gaatgccaac ggttggatgc aatacccgac tggtgcgcat tttgatatcg acaccctgcg
    10381 tatggagatg accagtttca gcgagctggt ctttaacccg gtcagccagg tgaaatttgt
    10441 gcacaccgta atggcgggat acgtgaccgg ggctatgttt attatggcga tcagcgcctg
    10501 gtatttactg cgcggacggg agcgcgatgt cgcattacgc tcgtttgcca tcggttccgt
    10561 cttcggtact ctggcgatta tcggtacgct gcaactcgga gacagttctg cgtatgaagt
    10621 cgcgcaagta caaccggtaa aactggcggc gatggaaggg gagtggcaaa cagaacctgc
    10681 acctgcaccg ttccatgtgg ttgcctggcc ggaacaggat caagagcgta acgcctttgc
    10741 catcaaaatt cccgcgctgc tagggatcct cgccactcac tcattagata aacccgtgcc
    10801 gggtctgaag aatttgatgg ctgaaaccta ccctcgcttg caacgtggac gtatggcctg
    10861 gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg
    10921 ggagctggaa ggcgacctgg gttacggcat gttgctctcc cgctatgcgc cggatatgaa
    10981 tcatgtcaca gccgcacagt accaggcggc gatgcgtggc gcgatacctc aggttgcgcc
    11041 ggtattctgg agtttccgca tcatggtggg ctgtggttcc ctgctgctac tggtgatgct
    11101 gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat
    11161 ggcgctctgg agtttgccgc tgccgtggat tgcgattgaa gccggatggt ttatgaccga
    11221 gtttggtcgt cagccgtggg cgatacagga catcttaccg acatactccg cgcactccgc
    11281 cttaaccaca ggacaactgg ctttctcact gatcatgatc gtagggcttt acaccctgtt
    11341 cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt ctggggccga gcgcgatgca
    11401 gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa tcatgtttga ttatgaaaca
    11461 ttgcgtttca tctggtggct gctgattggc gtgatcctgg tggtctttat gatctccgac
    11521 ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga tgatgaacgt
    11581 cggatagtga taaacagcgt tggcgcgcac tgggaaggca atcaggtctg gttgatcctc
    11641 gcaggtgggg cattatttgc cgcctggccc agagtgtatg cagcggcgtt ttccggcttt
    11701 tatgtggcga tgattctggt gctgtgctcg ctgttcttcc gcccgctggc ctttgattat
    11761 cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg atgccggact ggtcatcggc
    11821 agtctggtgc cgccggtagt cttcggtatt gcattcggca acttgctgct cggcgtgccg
    11881 tttgctttca caccgcaatt acgcgtggaa tatctcggca gcttctggca actgctgacg
    11941 ccattccctt tattgtgtgg attgctcagt cttgggatgg tgattttgca aggtggcgtc
    12001 tggttacaac tgaaaactgt tggtgtgatt catctgcgtt cacagctggc gaccaaacgc
    12061 gccgcactac tggtgatgct gtgctttttg ctggcgggtt actggctgtg ggttggtatt
    12121 gatggctttg tgctgctcgc ccaggatgct aacggtcctt ccaatccatt aatgaaactg
    12181 gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg aatcgcccgt tttgtggatc
    12241 ttcccgctgc tggggttctt ctgcccattg ctgacggtga tggcgattta tcgtggtcgc
    12301 ccgggttggg gatttttgat ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc
    12361 atcacgctgt tcccctttgt catgccgtca agcgtgagtc cgatctccag cctgacgttg
    12421 tgggacagca cttccagtca gctgacgctg agcattatgt tggtaatcgt gctgatattt
    12481 ttgcccattg tgttgctcta cactctctgg agctactaca aaatgtgggg gcgcatgacg
    12541 acagaaactc tccgccgtaa cgaaaacgag ctgtactaag gagcaggaac aatgtggtat
    12601 ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt gttggtatgg
    12661 ctggacccgc gtctgaaaag ttaacgaagg taagcctgat gaggcgcatt agcgtcgcat
    12721 caggcaatca ataatgtcgg atatgaaaag cggaaacata tcgatgaaag cgatcttgat
    12781 cccattctta tctcttctga ttccgttaac accgaaatct gcattcgctc agagtgagcc
    12841 ggagctgaag ctggaaagtg tggtgattgt cagtcgtcac ggtgtgcgtg caccaaccaa
    12901 ggccacgcaa ctgatgcagg atgtcacccc tgacgcatgg ccaacctggc cggtaaaact
    12961 gggttggctg acaccgcgcg gtggtgagct aatcgcttat ctcggacatt accaacgcca
    13021 gcgtctggtg gccgacggat tgctggcaaa agagggctgc ccgcagccag gtcaggtcgc
    13081 gattattgct gatgtcgacg aacgtacccg taaaacaggc gaagccttcg ccgccgggct
    13141 ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc ccgatccgtt
    13201 atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga ctgacgcgat
    13261 cctcagtagg gcaggagggt caattgctga ctttaccggg catcggcaaa cggcgtttcg
    13321 cgaactggaa cgggtgctta atttcccgca atcaaacttg tgccttaacc gtgagaaaca
    13381 ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa
    13441 tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat ttctcctgca
    13501 acaagcacag ggaatgccgg agccggggtg gggaaggatc accgattcac accagtggaa
    13561 caccttgcta agtctacata acgcgcagtt ttatttgctg caacgcacac cagaggttgc
    13621 ccgcagtcgc gccaccccgt tattggattt gatcatgaca gcgttggcgc cccatccacc
    13681 gcaaaaacag gtgtatggtg tgacattacc cacttcagtg ctgtttatcg ccggacacga
    13741 tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc caggtcagcc
    13801 ggataacacg ccgccagggg gtgaactggt gtttgaacgc tggcgtcggc tcagcgataa
    13861 cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc gtgataaaac
    13921 gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg accctggcag gatgtgaaga
    13981 gcgaaatgcg cagggcatgt gttcgttggc cggttttacg caaatcgtga atgaagtacg
    14041 cataccagcg tgcgctctcc atcaagacaa ataaacattt gccgctgttg gtccacgcag
    14101 gccatttacg cggcaaaatt ccacgcgtaa tcctggtata agcacttctg cgtcgcgggg
    14161 agtgaatgcg gaaatatgga cctggacttc tttacgaccg tcggagggga taatgaatcc
    14221 tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg acaaacaaat
    14281 tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc cagcgatatt
    14341 taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc
    14401 tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc
    14461 aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag
    14521 ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt
    14581 ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc
    14641 aaagacgttt tcgtccattt cactgccatc cagagcaatg aattccgcac gctgaacgaa
    14701 aatcagaaag ttgaattttc tattgagcag gggcaacgtg gtcccgcggc agcgaacgtt
    14761 gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc catgttgcca
    14821 tggttcacag taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggtgcaaa
    14881 gtgcattttt tgtaaatcga cgatgatcac ctttgataac gttgcgctgc aaatacgcac
    14941 tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta
    15001 tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg
    15061 aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca
    15121 ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag
    15181 ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg
    15241 aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc
    15301 gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg
    15361 cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgatt gcggaattaa
    15421 gcgttggtac aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc
    15481 aatcgccgca aacataatcc ccataaccag tagcgtcatt aaccagccag acaacgcaaa
    15541 ggctttttta tttgccgcag gtttttgcag tgaaaaggcc aatgttgagg ctaccggaca
    15601 tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc
    15661 gaccgggatc cgtgatgggc aactttttgc gcatttgcca caatcgatac aactttcggc
    15721 attgcgacga atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg
    15781 gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca aaacgatcat
    15841 gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa cgtccatcac
    15901 cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat
    15961 atagagaaaa aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg
    16021 gggaaggaca aattgccgac caaacagttt gttaccgaga tcgccgatta attccgaaag
    16081 cgtaccaacc gggcataacc atgaacaaaa ggcctttttg agtaatagac tgatgacgat
    16141 aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgcaag
    16201 gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac cacccggtct
    16261 ggtgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc agaacgtgat
    16321 ggcaatataa atattaattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc
    16381 attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gttgccagcg
    16441 ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact
    16501 attctagttg ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatta
    16561 accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg ggggcagata
    16621 atggctgctg ctccagttgt gcgcattgct ggctggcgct acgcattccc agactcgagc
    16681 aactgctttt tagctgatgt gcggcacgct ttattttctc gttatcctga ctggcgcggg
    16741 caatgtcgat ttcatcgaga agcggcaggg catgttgttt aaataatatc agccattcgt
    16801 ggatcttctc cgcccccatt aaatgagcat cttcattgag ttgcgataca tccagtggtt
    16861 gatcgttatt gacttgcagt tggagatagt gcgccagtaa ttgaccgagc acttcacgcg
    16921 gcaccggttt agggataatc ccgcgaaata gcgaactggt acgctgtcgc agtgtttcgt
    16981 caatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt gccagttgtc
    17041 gggcaagcgt aatgccgtcg atatccggta gatcaaaatc caccagtgcg gcagcaaacg
    17101 gttcactatt ttgcagtgtc tctaaagcct gcgcggcatt tcctacggca acgacctgcg
    17161 ccccactgct gttcaacatc tcaacggtaa tccgctgggt tagcgggtta tcttcaatta
    17221 acagcaaacg taaaccgtcc agacgcaccg cctgattgac tgtttttggc acgggtgcag
    17281 tggcaacacg taacggcacg cgtaaacaaa aacagcttcc aacctccggc gtgctggtgg
    17341 cgctcaattc gccgcccatc gcctgggcca gacggctact gatagtcagc cccagcccgg
    17401 tgccgccgcg tttgccgcta acctgaacaa atggctggaa gatttcagcc agtttcgcgg
    17461 gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgttcgcca tcaatgcgac
    17521 tacgcagaat aatgtgccct tcgtcggtga aacgcagggc gttgctcaac aggttggtaa
    17581 tgacctgacg aatacgccgc ggatcgccca ttaacgcagt cggtacatcg tcggcaattt
    17641 ctgttgccag gcgaatcggg cgacctttca cccttccgct cattaattgc agggtacttt
    17701 ccagcagcgg gcgcggttca aagggttcat cgctgaccga aacattcttg ccgcctgctt
    17761 cgatagccga ataatcgaga atatcgttga ggatggtcag caacgattca ccagagtcgg
    17821 taatcgcccg caaatcatcg cgctgggcgt taagtgcggg gttatctgcc agcagctgtg
    17881 cggtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc gccagaaacg
    17941 ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt tctatcacca
    18001 gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg cggtggcgat
    18061 tcagtgcatg aacactgctg cgaaacgcat ccatcagccg cccgatggta tccagttccc
    18121 gtacgccagc ggtttccggg aaaggggagt cgatatcacc atccagcaag cgttgcagcg
    18181 cctgcgtttg ttctgcaaga gggcgcgtga ctgagcgata aaccacgcgc cagaggatca
    18241 gaatcagtgc gcaaagagaa actaatccca gcaataacag gctgtattgc ccgcgtgcac
    18301 tggctttttc cagatgagcc agcccgtgct gattacgcag ctcaatggtg tcgaccagct
    18361 gactgacttc gctactaaac tgcgcgaatt gggcgatgtt attttgggcg agagtttgca
    18421 ggtgattgct gatttcactg tcctgctgat acagcgccag caaatcgcta tattggctaa
    18481 cggtagttaa cgttgttgct acctgagtac gaacacccgg atcttcaatg cgtatttgcc
    18541 gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc gcatttttct
    18601 ggatctgttc cagccccaga ttcatcacca tttgctgcac ccgcagggcg ctaaggcgca
    18661 gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg agtgcacttt
    18721 ctgcggcttg acgctgatgt tgttcgatca aatcgtaaat cccggcctgg gtcgctccgg
    18781 cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatttca tcggcggcgg
    18841 cgactatctg ctgactgagt tgctgttgtt gctggcgaag ttgcaaacgc tgccccacca
    18901 gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca atagcggtgg
    18961 tgtcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcaga ctttgtgcgg
    19021 tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc aggttctgcg
    19081 cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc atggtaggaa
    19141 tcaatgcctg agtgttgtct ttttccacct ggctgataaa gcgcaggttg taccatccca
    19201 ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag agtcttcggg
    19261 tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg caaagcggaa
    19321 tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg ctatttttac
    19381 ttctttccct tttcatgtta tcggcatttt cggctgataa cctgttgcgc tggcatgatg
    19441 cgcagcattt cacggtgcaa gcctctacgc cgcttaaagc caaacgcgca tggaaactgt
    19501 gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat ggtatgcagg
    19561 aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc tacagccagt
    19621 tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag gccattttgc
    19681 tcggtagtag cacgacgtca tttcccgatc tgcaaaagca ggtagcaaat ctgccggtga
    19741 tcgaactggt aaatgctatt gatgctcccc acgtgaaaag ccgcgttggt gtgccctggt
    19801 ttcagatggg ctatcaacca gggcgatatc tggtgcaatg ggcgcacggt aaaccactga
    19861 acgtgctgtt gatgcccggc cccgataacg ccgggggcag taaggagatg gtagagggtt
    19921 ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctc ggtgataacg
    19981 atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccatcca gaaatcgacg
    20041 tcgttgccgg aacggccatt gcggcagagg cggcaatggg ggaagggcgt aacctgaaaa
    20101 cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg ttgaagcggg
    20161 gaagagtgat tatggctgcc agcgatcaaa tggtctggca aggggaactg gcggttgagc
    20221 aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca ccgattttag
    20281 tcatgacgcc gaaaaatgcc gaccgcgaac atatccgccg ctcgctgtca ccagggggat
    20341 ttcgtccggt ttatttttat cagcacacat cagcggctaa gaaataacct tcgccatgtt
    20401 gtgtcaccag taaatccgca ctgagtttat ggcgtaaacg acgaattaac acatcgacgg
    20461 tgcgcaggtc agggttttcc acccgacgcg cagagagcat acgcagcaga cgttcacggc
    20521 tgagaatttc gcccggatta gtcacaaatg ccaccagcat ttcatactct gcgcgggtca
    20581 gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg caataaccgg
    20641 caaagcgata gcagttgtcc tgagtgtgcg gttgcgcctg tcgcgcgagg tcgattcgcc
    20701 agagcaaatt tttcactcgt accaccagtt cgcgcagctc cagcggtttg gtgacgtaat
    20761 cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt ccggtaacca
    20821 gaataatccc taccgttgag cgttctcgca gggcgcgggt taacatcagg ccattttcat
    20881 cgggcaaatt gatatccagc agaatcaaat ccaccggctg attctgcata atttcccgca
    20941 gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag taagattgta
    21001 atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc atcagagggt
    21061 tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc ctgaccagtg
    21121 ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataatttta caggggctat
    21181 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
    21241 ggtcgctact gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
    21301 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
    21361 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
    21421 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
    21481 ttaccagacc tttattgccc actccattga tacacctgag aaatttgaag ccaaacgcgc
    21541 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgccgctc
    21601 ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg cgcgtcagat
    21661 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
    21721 gttaccggat atgagtagtg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
    21781 tgatagtggt gacacgctgt actccattga tattaagccg atttatgcgg cgaaaggcga
    21841 taaagaagcg tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
    21901 cgactggctg caaattgaaa tcactggctg gacggaaagt gcaggacgtc agcgtgtact
    21961 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
    22021 ggtgaaaaca ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
    22081 ggccactgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggctta
    22141 tgcggattcg ctgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatatccca
    22201 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagcctcga
    22261 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
    22321 taaagctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
    22381 tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat gctagggccg
    22441 tcattgttaa cgccgcgccg tgcgactgcg gcgcaagcgg cgactgaggc tgtcatctcg
    22501 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt gaaggatggt
    22561 cgctttgtgg cagcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
    22621 ttgccggatc atgtacacaa cgcggcgcgt attcgttacc cgatggtacg cgtggactgg
    22681 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
    22741 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
    22801 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
    22861 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttagtac gggcggagat
    22921 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat ggaagtgtat
    22981 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
    23041 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
    23101 tattacgagc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat cagcatcgat
    23161 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
    23221 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcgc atacgctgta cagtgaaaac
    23281 ctgtacgaca aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt cctgccgtat
    23341 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
    23401 attgatgccg aaaccattcg tgggctggca cggcagatgg cggcgaacag aacgcagatt
    23461 attgctggct ggtgcgtaca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtc
    23521 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ttttggctgg
    23581 cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttttgagtgg tttctccggc
    23641 tctacgtcga ttccgcctgt tcacgacaac agtgactaca aaggctacag cagcaccatt
    23701 ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt
    23761 aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ccggaactaa cccgttccat
    23821 cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac ggttatcgcc
    23881 atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc tgcgaccacg
    23941 cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaatcgtgg catcatcgcc
    24001 atgaaacagg tcgtgccgcc gcagttcgag gcgcgcaacg acttcgatat tttccgcgag
    24061 ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga aatgggctgg
    24121 ctgaaacgca tctggcagga aggtgtacaa cagggcaaag gacgcggcgt tcatctgcca
    24181 gcgtttgatg acttctggaa taacaaagag tatgtcgagt ttgaccatcc gcagatgttt
    24241 gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac gccgagtggc
    24301 ctgattgaga tctactcgaa aaccatcgct gatatgaact acgacgattg tcaggggcat
    24361 ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ctggctcgca gaagtatccg
    24421 ttgcatctgc aatctgtgca tccggatttc cgacttcact cgcagttatg tgagtcggaa
    24481 actctgcgtc agcaatatac cgtagcgggt aaagagccag tatttattag cccgcaggat
    24541 gccagcgcgc gcggtattcg tcacggtgat gtggtacgcg tctttaacgt tcgtggtcag
    24601 gtgttggctg gagcagtggt ttctgaccgt tatgcacccg gcgtggcgcg aattcacgaa
    24661 ggggcatggt acgatccgga caaaggcggc gagccagggg cgctgtgcaa atacggtaac
    24721 cccaacgtct tgaccatcga catcggtact tcgcagctgg cgcaggcgac cagtgcgcac
    24781 actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac ggcgtttaac
    24841 ggccccgtgg agatggtggc gcagtgcgag tatgttcccg cgtcgcaggt gaaatcatga
    24901 ccacgctgac agcacaacag attgcctgtg tttacgcctg gctggcgcag ttgttctccc
    24961 gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct gaatggtttt
    25021 cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa tgggctggaa aacagtattg
    25081 ccactctgac agtacgtgac gatgcccgtc tggaactggc cgctgacttt tgcggcctgt
    25141 ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa caggacgagc
    25201 aagagattaa acgcttgtta gttgaggcgg ggatggaaac cagcggcaat ttcaacgaac
    25261 cggcagatca tctggcgatc tatctcgatt tgttgagtca tctgcatttt tcgctgggag
    25321 aggggaccgt tcctgcgcga agaatcgacg gtttgcggca aaaaacactg acggctctgc
    25381 gggaatggtt accagagttt gctgcgcgtt gtcgacagta tgacagcttt ggtttttacg
    25441 cggcactaag ccagttattg ctggtgttag tggagtgtga ctaccaaaaa agataatgcc
    25501 gtttgtaggc ctgataagac gcggcaggcg tcgcatcagg cattagtgcg cagttgccgg
    25561 atgcggcgag aacgccttat ccggcctacg ggaacagtgc tttttgtggg cctgataaga
    25621 tgcgttaagc gtcgcatcag gcattatggc gcagttgccg gatgcggcat gaacgcctta
    25681 tccggcctac gggaacagtg ctctttgtag gcctgataag acgcgttaag cgtcgcatca
    25741 ggcaacaaac tcacgggtga gccacaaacc gggaaagccg ctggcgcagc aggcggtttt
    25801 cctgcttcag gtgcgcaata tcatccatta acgtcagcgc caccgcgatc cccggccagt
    25861 ccagagccag ttcatgacgc aggcgtaccg cgcgttgcac cacaatggcg gcatggtcgt
    25921 caaataccca ggttgtctcc tggatctcac tcggttcaac cacccccaaa ccgacaattt
    25981 cattcaactc ctcttcagag atgccggtat gcaggcaaaa ttcggtaata gtaaaagtca
    26041 ccgtaacatt agccattatg ctttccccca atctttacgt ggatcaaaag acgactgggc
    26101 gtctgccagt tgctgccaca gcgcggcagt gttttcatcc ggtttcggcg gcatcacgat
    26161 tttcagtacc gcatacagat cgccggtctg ttttttgctc accagacctt tgcctttaac
    26221 gcgcaatcgt tgcccggcct ggctgccagg cgggatagtc agcaaaatgc tttctttcag
    26281 tgttggaacg gtgactttag tacccagcgc cgcttcccac gggctaaccg gcaccactat
    26341 ttccagatcc tggccgacaa tatcaaacag cggatgtggc gcaatgtgaa tcaccagcca
    26401 caggtcgcca tttggaccgc cgttttcgcc cggcgtcccc tggcctttca ggcggatgcg
    26461 ttgaccattg ccgacgcccg ccgggatctt cacattcagc gttttcggga tttcctgttc
    26521 gatcatgcca aaggcgttat aaaccggcag gttatagctg atggtacgct tatgctcagt
    26581 aagcgtttct tcgaggaaaa ccgccacttc gatttcaata tcgtggccgc gtgtggcggg
    26641 gcgttgacgg ctctggcggg catgctgacc gaaaattgac gagaagatat cgtcaaaatc
    26701 ttcggcgtta aaactctgac cgtcgccatg atggaactga cggttaaatt gcggatcgtt
    26761 gcgatgttgc cacatctgat catactcagc gcgacgttgt tcatcactta acacttccca
    26821 ggcttcggcg acctctttga agcgggcttc ggcatccggt tctttgctga catcaggatg
    26881 gtatttgcgg gccagtcgac gataggcggt cttgattgtc ttgagatcgt ccgtcggttt
    26941 cacgcccatg atggcgtaat aatcctttaa ttccatagcg ttatctcgcg taaatcaaca
    27001 caaattgaag gaacccctgt aaggtaactc ctataagtgt agggtaatcc tcaaaatttc
    27061 atatgccaac acagaatatg ttattgaaat catcgcggag gggaggttgc ctcaaggtgg
    27121 gaggaacata ttttatttac ctcactcctg aacttttggc tcatgcttac aagttaaaca
    27181 ataaggaaag gaatgcacaa ctcgactatt taaatcgtta tgcaatttaa tgataaagac
    27241 atgtgctaaa aaccattgct attagtctca cattcttttt ttattggtaa tggtaacgcc
    27301 gctgattttc tttatagcta ctgatgtaat cattagcggc gtaaacgagg ccggggctaa
    27361 tcaggcatag tttgcgtcaa aacttgcctg tttttaaagg ggaatataga aaaacaggcg
    27421 ttcaacgagc cattttgcga acctgttccc ggaaaaatga catatttctg tcacactctt
    27481 taattattga taacaaaaga ggtgtgagga atgaacaaaa cgctaatcgc cgcaactgtg
    27541 gcagggatag ttttactcgc ttcaaatgct caggcacaaa ccgtaccgga aggctatcag
    27601 ctacagcaag tccttatgat gagccgtcat aacttacgtg cgccgctggc gaacaatggc
    27661 agtgtgctgg agcagtcgac gccgaataaa tggcctgaat gggacgtccc cggtgggcag
    27721 ctcaccacta aagggggcgt gctcgaagtg tatatgggcc attatatgcg tgaatggctg
    27781 gcagaacagg ggatggtgaa atcaggggaa tgcccgccac cggacaccgt ttatgcctat
    27841 gccaatagtc tgcaacgcac cgttgcgacc gcacagttct tcattaccgg cgcattcccg
    27901 gggtgtgata ttcctgttca tcaccaggaa aaaatgggca ctatggaccc cacctttaac
    27961 ccagtgatca ccgatgattc cgccgcattc agtgaacagg cggtggcggc aatggagaaa
    28021 gagctcagca aactccagct taccgacagc tacaagctac tggaacaaat cgttaactat
    28081 aaagattctc ctgcgtgtaa agagaagcaa caatgttcgc ttgtagatgg caaaaatacc
    28141 tttagcgcga agtatcaaca agaaccgggc gtttccgggc cgctgaaagt cggcaactcg
    28201 ctggtagatg cgtttacttt gcaatactac gaaggttttc cgatggatca ggtggcctgg
    28261 ggagaaatca aatctgacca gcagtggaag gtgttgtcga agctgaaaaa cggttaccag
    28321 gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg cgaaaccgct ggtcagttat
    28381 atcgacaaag ccctggtcac tgaacgcacc agtgcaccga aaatcacggt attagttggg
    28441 cacgactcca acattgcctc tctgttaacg gcgctggatt tcaaaccgta tcagttgcat
    28501 gaccagaacg aacgcacgcc gattggcggc aaaatcgttt tccagcgttg gcatgacagc
    28561 aaagccaatc gcgatttgat gaaaattgaa tatgtgtacc agagtgcgga acagttacgt
    28621 aatgccgatg cgttaaccct gcaggcacct gcgcagcgcg tgacgctgga attaagcggt
    28681 tgcccgatag atgccaacgg tttttgccca atggataagt ttgatcgcgt attgaatgaa
    28741 gcggtgaaat aacagaaaac tcccccgcgt gaagcggggg agtcgctggt taaacgtttt
    28801 tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt atcggtctta gcaaaggctt
    28861 tgactaacac ttcatcgcta ccttcttccc aaatcttttc cgccattttt tcgtcgtacc
    28921 cggcgacttc gaaaatggcc tcggctattt ccggcgacgt attgcgcaga gatgcccatt
    28981 caccgacgtg atgagctttc gcttcttgag ttggcatgcg tatcctcctg ttgaagatta
    29041 gccgttaagt ttaactgcca gacctgcgac atattcccct tgataacgag caatagacag
    29101 ttcttcctgg ctgggctggc gtgaaccgtc accgcctgcg atggtggttg cgccgtacgg
    29161 cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc gctgcgtagc caatggggac
    29221 aatcaccatg ccgtgatgcg caagggtcgt ccaggtggat gtgatggttt gttcctgacc
    29281 gccgccagta ccggtggaac taaagacgct cgccagtttt ccgtatagtg cgccggaagc
    29341 ccacaggccg cccgtctggt cgaggaaggt acgcatttga ccggacatgt taccaaagcg
    29401 ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt tcttgcgggg ttgcaaccgg
    29461 tgcagtttgc gttttaccgc ctgctttttc aaataattgc ggcggcatgg tttccggtac
    29521 acgcttaacg acaacttctg cgccatccac tttgcttgca ccctcagcaa ctgcgcgagc
    29581 catcgtttca atatgtccgt acatggaata ataaagcacc agaactttag ccatttctaa
    29641 ccactcctcg tgttatctct attccgtagc gattcgctac cacttattta aagataagac
    29701 gtccttttca gagtgcaaat ttcacaacca cttatttgat ttataacaac tttcacaagc
    29761 acgtaatttt gtcgcaaaat gacacatttt tatctcatcg cgttttttta atcataagag
    29821 cggcttatgg ataattattg gagatgatat ctattctcgc taagaagctg ttgcaggata
    29881 ttaccaaacg cggatctgcc cgcgtcagtt cactaagctt agttccacgt agcgaaaata
    29941 tggcagccgc catacgccgc gttaattcta tgcaatatga tgtctatacc cagacggagg
    30001 tcagtaatgg caaaccatcg aggcggttcc ggcaattttg cagaagaccg cgaaagagca
    30061 tcagaagcag gtaaaaaagg tggacagcac agcggaggta atttcaaaaa tgacccgcag
    30121 cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc acggtaaaag cgacaactag
    30181 ccgggctaat caatgacgca tgcatttttg tctgtagctc gtcaaaacgc catcaccgcc
    30241 ggtagccccg gcggttttct gtttatggct tctgatgaac aacttctggc ggtggaacgt
    30301 caaccaacct tctgcttaac aacgcattga gtaaaatcgc gccaaaggtg gctgtaccga
    30361 tccctcccaa cgtaaaaccg cccagtgtga gagcaaaatc acccgcaccc agcactaagg
    30421 ttactgcgac cataatcaaa ttaccgttct ggcttaaatc cacactgttt tgtacccaga
    30481 tccgtgcgcc tgcgacggca atcagcccga acacaacaat tgatgcacca ccaatcaccg
    30541 cagccggaat agtatggatc agcgcgccaa atttcggcga aaagcccaac agcatggcga
    30601 tgacggcagc ggcaacaaac accagcgtcg agtagacttt tgtcacggcc atcacaccga
    30661 tattctcggc gtaggtggtt atgccgctac cgccgacaga gccagaaagc atcgtcgcca
    30721 gcccatcgcc tacgaatgcc cgccccatat acgggtccat attgcgtccg gtcatcccgg
    30781 cgaccgcctt gagatgacct aagttttccg ccaccagaat caccgccacg ggcgcaatca
    30841 gcatcattgc ctgaccatta aaagcaggag tggaaaaatg tggcagaccg aaccaggcag
    30901 catggctgac gagagtaaaa tcgacggctt ttcccagccc taaaacgttg gtcatcacgc
    30961 catacagcag acaggcgaca attaatccta cgagaatcaa taatcgctgg atcatgccac
    31021 gggtaaatac ggccaccagc ccaatacaca gcaccgtcat taccgccatc cagctatcaa
    31081 aggccgaagc cgatacattt ttcactgcga taggcgctaa gttcaggcca atcgccatca
    31141 ctaccgcacc cgtcacaacc ggcggcatta gtcgttcaat ccagcgcgta ccgattttca
    31201 tcaccaccag gccaatgacg gtataaacca gcccacaggc gataatcccg cccagcgcga
    31261 tgctgatgtt cggattaatt ccctgaccat taaagcccgt cgcggcaatc accacgccga
    31321 caaaagccgc gctggaaccg agataactgg gcacgcgccc gccggtaata aagaaaaaca
    31381 gtaacgtgcc gatccccgac attaaaatgg aaagattggg atccagcccc atcagaatcg
    31441 gcattaacac cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt actgccgttt
    31501 gagcaaacgg caatcgttcg tccggcgcga ccacgccgct ctctgtagag gtcgatttta
    31561 actgccagtg aggaaaaccg aacattgcca tcagctgtct ccttaaggag gttaacaagc
    31621 agggcgcatc agcgcgtgat aactgcgatc gaaccacacc agcccgtagg gtgtggcgtg
    31681 acgatgaatc gcttcgatgg cgcaaaacaa aatgtcgtgg gtgccgacgc tcaccacctg
    31741 gctgatacgg cagtcaaacg aaaccagtgc cttttccagt tgcgggcatc cggtcacacc
    31801 agtctgccag cgggcggcgg caaagcggtg ttccatgggc gttttgccgc caaaaaggtt
    31861 tgaaagcggc tcctgcccgg cgctaagtgt atttacacac agcgttcgat tttcattgaa
    31921 taccggccag acggacgccc cacgattcag gcacaccagt aatgtgggcg gcgtgtcggt
    31981 cacgctgcag acggcgctgg cggtgaaccc ggcgcgcccg gctggaccgt ccgtagtgat
    32041 gatattgacc gccgcgccca tacaggacat cgcatcgcga aaggtttgtt gatcgacaat
    32101 gttcattgtt tgctccttac aacagcccgc aggcttcttc aaaggacaga cgtggcaggc
    32161 gcgcaaaaag tttgctggaa tcgccatagc cgatattaat cagcagatcg ctcttcagcg
    32221 tgctgcccgc aaaaaaggcg tcgtcaacgt attgacggtc aaagcccgac atcgggccgg
    32281 tatccagccc cagcgcacgg caggcgacga tcagataggc cgcctgcatg gaactgttgc
    32341 gaaacgctgt ttcttcggca agttgtggac tggaggtaaa ccaactgcgg gcatcgccgt
    32401 gaggaaacag ctgtggtaac cgttcataaa attcactgtc ccaggcgacg atagcggtga
    32461 cgggcgcggt cagggttttt tgcagattgc cgctggaaag cgccgggcgc agacgttctt
    32521 ttccttctgc agtgcggata aacacgatcc gggccggaga acagttagct gatgtcggcc
    32581 cccatttcat cagggcataa atctcccgta acgtctcatc gctgacgggg ggctcccgcc
    32641 agccgttgtg agtgcgggca tcggtgaaca gggtgctaag cgcacctggg ctaacggctt
    32701 cgttcatagc aattccttac agagcggctt cacggtgatg taacaggctg gcaagcccgt
    32761 tgagtaacag agcattaaac gtttcgggat cggtcacgtt gcaggcgtgt ccgccatagc
    32821 gcatcaccat tttctggcta tcgggcaggg cggcatgaag ttcactggaa catgctgttg
    32881 gcaccagcag atcgtcactg gcgcagatga tttgcaccgg gcagcggata cgatccgcat
    32941 ggtgactaaa gtcagcgcgt ttgagggcgt taagtcgacg cagtaaatta tttttgccct
    33001 gaaaatgcgc cagtgccagc gcatcttctg cctcaaggcg aggtgcgcgg gccgccatcc
    33061 agtcggcggg atagaggaac aacggctgcg cttccaccca tgcctgcgcg ccgccgctat
    33121 gtagtaactg ttcgcgaacc tgaaaacagc ggcgcgtatg ggtgtttatt cgtagccagc
    33181 cgttaacgct gaccagcaca gttaccgacg cgggataatc cagcgccagc tgcattccca
    33241 ccagcgcacc gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg gctaccagcg
    33301 cctgatgcag ttccgctgcc atctgggcga tactgtaatc ttctgccagc gtgtcgggat
    33361 tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgatcc agcaccgcca
    33421 gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc actacgggcg
    33481 catcagcata agggggaggt gagagtgaaa gtttcatcgc ggcctcactt ggcgatatgc
    33541 gcaattgtgg cgatttccac cagcgcgtca ggttttacca gtccgcactg aatgcagaat
    33601 cgcgccggtt tgtcacccgg gaaaaactcg gcgtagattt cgttaatcgc ggcgtaattt
    33661 ttccagtcgg taataaagat gctgttgaaa gtcacatccg ccatcgtgcc acccgccgtc
    33721 tcgatgacct tgcggatggt ttccagaacg tggcgggttt gcgcctttgg gtcatcggca
    33781 aacagcacgt tgttatgttg atcaaaagcc agcgtaccgg agacatacac cacgccatca
    33841 gccagcgtgc cgggaacgaa gggggccagc ggtgcgctgc tgccagcagg aataattacg
    33901 gattttggca tcgttaaact ccttaagcga tacgagcaaa ggacgtggaa gaaagcgcgt
    33961 cgcagaacgt ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg aacaacgcag
    34021 ctttctgtgc aaattccggc cccgcctggt gtgttgcgtc ttccagcacc acgccgaaat
    34081 actccagaaa aaagccgtcg cgtagcgtcg attcgacgca gacgttggta gcgataccgg
    34141 tgaaaaccag atggcgtatt ccacggctgc gcaaaatgct gtccagcggc gtattgaaga
    34201 aaccgctgta gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc agttcatcca
    34261 ccagttgata atcccaggag cctttcgcca gcaatttccc ctgcagctgc ggctgcttac
    34321 gcatggtttt cagggcgttc gatttatgaa aattcggtga gccggggccg ccagcctcga
    34381 catactgttc atcccagcca ttttgaaacc agatgatcag catccctgcc gctcgcgctg
    34441 cggtcacggc ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca tcaaacccgg
    34501 cgagatctaa gtagccgcct ggcgtggcat aagcgttttg catatccacc acgatcagcg
    34561 cactttgctg cggatcgaag gtaatggctt ccggtcgagc ggttaaggtc gtcatcatgc
    34621 cacctcctga gtcagcgcag ggagatgggc gcggcactgc atcagtggtt gaatgcgctc
    34681 gccgaaggtt tcgattcccg acagaaaatc atcgaaggtc aacagcacgc cttcggcacc
    34741 aggcacgttt gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac cgactaacgt
    34801 ccccatattg atgtttaccg ccgaagtggg atcggccatc tgacggacgt tggtgtcagt
    34861 acctgagcgg gtatctttct gactttgttc ggttagccag cttaacgcct cttcatccgc
    34921 gcccgctttg tagtgttccc attttgcacg agcggcatcg tcggtttcgt cggcaatcac
    34981 cataaacaac acataagagc caacgtcgcg tccggtttgc tctgcggcct gtttcatccg
    35041 cgcagcggtc ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac agaagttaaa
    35101 atcggcatac cgggcggaga acgccatgcc agcgtcgctt tgcccggcgc agatcacttt
    35161 catggggaca ctcggttgcg gactgacgcg acaatcatcc atagtgaaaa aatcgccttt
    35221 aaaatcgctt ttccccgagc cccacaggtc gcgcagcacc tgaacatatt cggtgagata
    35281 gtcgtaacga cgggagaaat agtcatcgcc aggccagatc cccatctgct catactcggg
    35341 cttctgccag ccagtcacga ggttgacgcc aaaacgcccg ccagagatgg agtcgatggt
    35401 tgcggccata cgggcgacga ttgccggagg taacgttaag gtggcagctg tcgcgtatat
    35461 ctgaatgcgc gaggtcacgg ctgccagccc cgccatcaag gtgaacgact caaggttgtg
    35521 atcccagaac tcagttttgc cgccaaagcc acgcagtttg atcatcgaca gggcgaaatc
    35581 gaaatggtag tgctccgctt tttgcacaat ggctttattc agttcaaatg tcggcatgta
    35641 ctgcggcgcg tgggtcgaaa tgagccagcc gttgttgcca ataggtacga atacgccaat
    35701 tttcatcatc aacctctctt cgtctcgtaa agtgaaagtc agacggggcg ctgcatcctg
    35761 catatccttt tcagccgcgt aatggcttgt ttgcaaagcg gatgccagtt tttaaaaagt
    35821 gaatgttatt aatgtgttaa tgttaagttg tctaaaatgc ctgaaaaaaa gtggactaaa
    35881 cggtcaaaac agttgcacat aaaacatgca ttcgtgcgcg atgagagtgc agaaggtcga
    35941 ggccgggcgg gggttttgct atcctgttgc caatctacaa gaggggagag cgcatgacgc
    36001 aaggcgcagt gaaaacaacg ggtaaacgtt cgcgcgcagt gagcgcgaag aaaaaagcga
    36061 ttcttagcgc agcactggac actttttcac aattcggttt tcacggcaca aggctggagc
    36121 agatcgcgga gttggcgggt gtttcaaaaa ccaatctgct gtattacttt ccgtcaaaag
    36181 aggcgctgta tatagccgta ctgcggcaga ttctcgatat ctggctggca ccgttaaaag
    36241 cgtttcgaga agatttcgcc ccgctggcgg cgatcaaaga gtacatccgt ctgaagctgg
    36301 aagtttcccg cgattatccg caggcttcgc gcctgttctg tatggagatg ctggcaggtg
    36361 caccgctgtt aatggatgaa ctgacgggcg atttgaaggc attaattgat gagaaatcgg
    36421 cgctgattgc cggttgggtt aaaagcggca aactcgcgcc gattgatccg cagcatttga
    36481 tttttatgat ttgggcttcc actcaacatt acgccgattt tgcccctcag gtggaggcgg
    36541 tgacaggcgc gacgttgcgc gatgaggtat ttttcaatca aacggttgaa aacgtgcagc
    36601 ggattattat tgaggggatt cgaccacgct aatggtgccg gaggaaggtg taacatcctc
    36661 cggctacctg tttaaccgat cgtcattaaa ctggcgttac cgccagcggc agcggtattc
    36721 acactcagcg aacgctcgat atacagccgt tccagaagga tattgctttc gccacgggca
    36781 aaaccctgca ccgaaacaat tgcaccatcc cgcgcggcaa cggcttcaca caatgcgcga
    36841 agctgatccg aatcaccgtg gaagatcacc gcatcaaatg gttgagcggt tatattttcc
    36901 gctttcgcca gttgaatacg ttcgctgact gccgatggca atgccttcac taactgacga
    36961 tgcagcgcat tatccggcca cagcacctgg ctacccaccg ccagcacggc ggcgagctga
    37021 gtcagcgcat cctgttcatc atccgcaata cacaacacgc gctcacgcgg cagcagcgtc
    37081 caggtgttgc gttcacccgt cggccctggc aacaatcgtt gtgttcctgc ctgcgccaac
    37141 tcgccatatt gcgtacataa cgcctgcaat tctggacggt ttgccgccca ttcccgcagt
    37201 gcatttagcg gctgagtcaa tgcggctttc aactgcgcat cgaccggata ctccgcatcc
    37261 tgacgcgcga gcgtcaccgc cagcgcactt tccgggcgat tcgccagcag acggtagaga
    37321 tagagcggac cgcctgcttt cggcccggtg ccggacaatc cttcgccgcc gaacggttgc
    37381 accccaacca ctgcgcccac catattacgg ttaacgtaca gattaccgac atgcgccgag
    37441 ccagtgacct gggcgatggt ttcatcaatg cgcgtatgga cgccaagcgt cagaccataa
    37501 ccggaagcgt taatctgctc gatcagctct gggagctggt tacggttgta acgcaccaca
    37561 tgcaacaccg gaccaaagac ctctttttgt aattcggcaa agtcatccag ttcgatcagc
    37621 gtcggagcga caaaggtgcc gctttgccat tcacgggcat cttcgctgtt ttcccgcacc
    37681 gcctggaaca cctgacggcc tttactacgc atggtctgaa tatgacgctc gatattggct
    37741 ttcgcttcgc tatcaatcac cggaccgata tcggtggtca ggcgacccgg attacccatc
    37801 cggcattcgg ccattgcgcc gcgcagcatt ttcagcgtgt ggtcggcaat ctcatcttgc
    37861 aggcacagca cgcgcagtgc cgaacaacgc tgacctgcac tgtcgaacgc cgaggccagc
    37921 acatctatta cgacttgttc ggtcagtgct gaagaatcga caatcatcgc gttcatgccg
    37981 ccggtttcag cgatgagcgg aatagggcga ccctgagcgt ccaggcggct ggcgatattg
    38041 cgctgcagta acgtagcgac ttcggttgaa ccggtaaaca tcaccccgcg cacgcgatca
    38101 tcacccgtca gttgcgcgcc cacggtttca ccccgacctg gcagcaattg caccacgcct
    38161 ggcggtacac ccgcttccag caaaatggcg atcccttgcg cggcaatcag cggcgtttgt
    38221 tctgccggtt ttgccagcac gctgttacct gccgccagtg cggcagcgat ctgtccggtg
    38281 aaaatagcca gcgggaagtt ccacggactg atacatacca caggccctaa tggacggtgg
    38341 gtttcgttag cgaaatcatc ccgcacctgt ccggcatagt agtggagaaa atcgaccgct
    38401 tcgcgcactt cggcaatggc gttactgaag gtttttccgg cttcacgcac cagaatacca
    38461 atcagttgct gcatctggct ttccattagc acggcagcgc gatgcaaaat tgccgcgcgt
    38521 tcagccggag gagtggcaaa ccagattggc gcgttattaa ccgcactttc cagcgcctgt
    38581 tctacttcac gcggcgtggc ttcacgcaca tagcccacaa tatctttcgg ttccgcaggg
    38641 ttaataacgg gcgacatctc acctgccgct accggttgtt ccagcattgg caaggactgc
    38701 catttttgca gtgcactatt gagcagggct gaagagagcg aggccagacg gtgttcgtta
    38761 gcgagatcca gccctgccga gttgtcgcgc ccgtgaccgt aaagatcgcg cggcagggga
    38821 attttcggat gcggtaatcc agtttgccct tcctgttgcg ccagtttttc tacagcagtg
    38881 actggatcgg cgaccagttc atccagtggc aaagaggtgt cggcaatacg gttaacaaac
    38941 gaggtgttag caccgttttc cagcaggcgg cgcaccagat atgccagcag cgtttcatgt
    39001 gtaccgaccg gagcataaat acgacacgga cggttaagtt tgccgtcggc aactttcccg
    39061 gtgacctgct catacagtgg ctcgcccata ccatgcaggc actggaactc gtactgaccc
    39121 gggtagtagt tttgacccgc cagttgataa attgccgcca gcgtatgggc gttgtgcgtc
    39181 gcgaactgcg ggtagattag atttggcacc gccagcagtt ttttcgcaca ggcaagataa
    39241 gaaacgtcgg tatacacctt gcgggtataa accggataac cttcaaggcc gtccatctgt
    39301 gcacgcttga tttcactatc ccagtacgcg cctttcacca ggcgaatcat cagacggcga
    39361 cggctgcggg tggcgagatc aatcaggtaa tcgatcacca acgggcagcg tttttgataa
    39421 gcctgaataa caaaaccgat gccgttccag cctgccagtt caggctcgaa acagagtttt
    39481 tccagcagat cgagggagat ctccaggcga tcggcctctt cggcatcgat gttaatacca
    39541 atatcgtact gacgcgccag cagggtgagt gatttcagac gcgggtaaag ctcttccatt
    39601 acccggtcat actgggcgcg gctataacgc ggatgtagcg ccgacagctt gattgaaatg
    39661 cccggacctt catagatgcc acgaccgtta gacgctttac caatggcgtg aatcgcctgc
    39721 tgataggaaa ccatatacgc ctgtgcgtct gcggcagtca gtgcggcttc gcccagcata
    39781 tcgtaagagt aacggaaacc tttctcttcc agtttgcggg cattggctaa cgcttccgcg
    39841 atggtttcgc ccgtgacgaa ctgctcgccc atcaggcgca tcgccatatc cacacctttg
    39901 cggatcagcg gttcaccgct cttaccgata atgcggttca gcgagcggga gaggctggct
    39961 tcgttatggg tggaaaccag tttgccggta aacagcagcc cccaggtagc ggcattaaca
    40021 aacagggacg ggctacgacc aatgtgtgac tgccagttac cgttactgat tttgtcgcga
    40081 attaacgcgt cacgggtggc tttgtcggga atacgcaaca acgcttccgc cagacacatc
    40141 agcgccacgc cttcctgcga tgacaacgaa aactcctgca ataacccctg aaccatacct
    40201 gcgcgaccac tggcgttttt ttgattacgc agtttatcgg ccagttgata cgccagtttg
    40261 tgcgcctgtt cagcaactgg ctgcggcagg cgagcttgtt ccagcagcat agaaaccgct
    40321 tcggtttccg ggcggcgata ggccgcggtg atcgcggcgc gggaaaccga ctggggcaat
    40381 atttgttcgg caaagtcgag gaaaggctgg tgtggttcct ctgccggagt cggtgcttca
    40441 tcgctctcat tggccgcgcc agaaagcagc gcaggtagct ccgggagagt atcgctgttt
    40501 tccagttgtt caagataaga aaaaatcgcc tgtttaatta accagtgtgg tgtgcgatcg
    40561 atacgtgtcg cagcagactt aatacgctca cgcgtcgcgt cgtccagctt aacccccatg
    40621 gtggtggttc ccatgccatt actcctgttg ttctgaaagg tgcaacttaa cgttatcgtg
    40681 aaatatccat gatgttgcaa ctttgtgcaa ccatgttaaa tgtgacctgc gtagcaagct
    40741 taaaaatgaa tgaaatgtta ataaacgaaa tcgatatgac agggattaaa aaaataactc
    40801 agactttttc tctgcggcag ttaacatttt tgaaaggtgc aaccgcaaaa aatgtgagag
    40861 agtgcaacct gatgaaaaat agtgtcgctg agcactaaaa tttaatgtaa atggtgtgtt
    40921 aaatcgattg tgaataacca gcgcttccgg caggatacgg tcgccctggt aaaacataaa
    40981 ctctgttacc ccgttccggt ggcagatata acggcaagtt tcgacattgc cgataataat
    41041 tttttggaga ctttagatgg ctattagcac accgatgttg gtgacatttt gtgtctatat
    41101 ctttggcatg atattgattg ggtttatcgc ctggcgatca acgaaaaact tcgatgacta
    41161 tattctgggc ggtcgtagtc ttgggccatt cgtgacggca ttatcggcgg gtgcgtcgga
    41221 tatgagcggc tggctgttaa tggggttgcc tggcgctgtt tttctttccg ggatttccga
    41281 aagctggatc gccattggcc tgacattagg cgcgtggatc aactggaagc tggtggccgg
    41341 gcggttgcgt gtgcataccg aatacaacaa taacgcctta acattgccgg attatttcac
    41401 cgggcgcttt gaagataaaa gccgcatttt gcgcattatc tccgcgctgg ttattttgct
    41461 gttcttcacc atttattgcg cttcgggcat tgtggcaggc gcgcgtctgt ttgaaagtac
    41521 ctttggcatg agctacgaaa cggctctgtg ggcgggggct gcggcgacga tcctttacac
    41581 ctttattggc ggtttcctcg cggtgagctg gactgacact gtacaggcca gtctgatgat
    41641 ttttgccctg atcctgacgc cggttatcgt gatcattagc gtcggtggct ttggcgactc
    41701 gctggaagtg attaaacaaa agagcataga aaacgtggat atgctcaaag ggttgaattt
    41761 tgtcgccatt atctcgctga tgggttgggg gctgggttac ttcgggcagc cgcacatcct
    41821 ggcgcgtttt atggcggcag attctcacca cagcattgtc catgcgcgtc gtattagtat
    41881 gacctggatg atcctctgcc tggcaggggc ggtggctgtt ggtttctttg gtatcgctta
    41941 ctttaacggg cacccggcgg tagcaggtgc ggtaaaccag aacgctgaac gcgtgtttat
    42001 tgaactggcg caaattctgt ttaacccgtg gattgccggg attttgctgt cggcgattct
    42061 ggcggcggta atgtcaacgt taagttgcca gctgctggta tgttccagtg caattaccga
    42121 agatctatac aaagcatttc tgcgtaaaca ggccagccag aaagagctgg tgtgggtagg
    42181 gcgtgtgatg gtgctggtgg tggcgctggt ggcgattgcg ctggcggcaa acccggaaaa
    42241 ccgcgtgctg ggcttagtga gctacgcgtg ggcaggcttt ggcgcggcgt ttggtccggt
    42301 ggtgctgttc tcggtgatgt ggtcacgcat gacgcgcaac ggtgcgctgg cggggatgat
    42361 catcggtgcg ctgacggtta tcgtctggaa acagttcggc tggctgggtc tgtacgaaat
    42421 tattccgggc tttatcttcg gcagtatcgg gattgtagtg tttagtttgc tgggtaaagc
    42481 gccgtcagcg gcgatgcaaa aacgctttgc cgaggccgat gcgcactatc attcggctcc
    42541 gccgtcacgg ttgcaggaag ggtaagggaa ggcgttttac cgaaaatttc acgtcataac
    42601 aggaagcagg cagagggcaa tcagtgattg ccctctgcgt tttttctcaa aatattttta
    42661 catcttctca tcattatcac tacatttcac ttgtattttt cttcatcgta atgataatcg
    42721 ctatcactgc gatttacttt tctttgcata gattgactaa gaaaaacgtt taagggtggg
    42781 tggcatgttt gttccgtttc tcattatgtt gcgcgaagga cttgaagccg cgctgattgt
    42841 cagtttgatt gccagctatc ttaagcgtac ccagcgaggc cgatggattg gtgtaatgtg
    42901 gattggcgtg ttgcttgccg ctgcgttgtg cctgggcttg ggtatcttca ttaacgaaac
    42961 caccggcgaa tttccgcaaa aagaacagga actgtttgaa ggcattgtgg cggtgatcgc
    43021 cgtggtgatc cttacctgga tggttttctg gatgcgcaaa gtgtcgcgca acgtcaaagt
    43081 acaactggag caggcggtcg atagcgcatt gcagcgtgga aatcatcatg gctgggcgct
    43141 ggtgatgatg gtcttttttg ccgttgcaag ggaagggctg gagtcggtct ttttcctgct
    43201 ggcggcattt caacaagatg tcggggtctg gccgccgctg ggtgcaatgc tcggtcttgc
    43261 tacagccgtg gtgcttggct tcctgctcta ctggggcggt attcgcctca atcttggtgc
    43321 attttttaaa tggaccagtc tgtttattct cttcgtcgcc gcagggctgg cagctggtgc
    43381 cattcgcgca tttcatgaag ccggattgtg gaaccacttt caggaaatcg ccttcgatat
    43441 gagcgcggtg ctctcaactc actcgctgtt tggcacactg atggaaggga tttttggcta
    43501 tcaggaagca ccgagcgtca gcgaagtcgc cgtctggttt atttatctca tcccggcgct
    43561 ggtggcattt gttctgccac cacgcgcagg ggcgactgcg tcccgctccg tgtagcaaat
    43621 acgacgcaaa ctcttgctta gttacaacat actttaaagg gatagtctcg tcatgaccat
    43681 taacttccgt cgtaacgcat tgcagttgag cgtggctgcg ctgttctctt ctgcttttat
    43741 ggctaacgcc gccgatattc cgcaagtcaa agtcactgtg acagataagc agtgcgagcc
    43801 gatgaccatt acggttaacg ccgggaaaac acagttcatt attcagaacc acagccagaa
    43861 ggcgctggag tgggagatcc tcaaaggcgt gatggtggtg gaagagcggg aaaatatcgc
    43921 ccctggtttt agccagaaaa tgacggcgaa tttacagcct ggcgaatacg atatgacctg
    43981 cggtctgctg actaacccga aagggaagtt gatcgtcaaa ggtgaagcaa cggcggatgc
    44041 ggcgcaaagt gatgcgctgt taagtcttgg tggtgcaatt actgcatata aagcgtatgt
    44101 catggcggaa accacgcaac tggtgaccga caccaaagcc tttaccgacg ctattaaagc
    44161 aggcgatatc gaaaaagcga aagcactgta tgcgccgacg cggcagcact atgagcgcat
    44221 cgaaccgatt gctgaactgt tctccgatct ggatggcagc attgacgccc gtgaagatga
    44281 ttacgagcaa aaagccgccg atccaaaatt caccggtttc caccgtctgg aaaaagcatt
    44341 gtttggcgat aacaccacca aagggatgga taagtatgct gaccagcttt ataccgatgt
    44401 ggtcgatttg caaaaacgca tcagtgaact ggctttccct ccttcaaaag tggtcggcgg
    44461 tgcagccgga ctgattgagg aagtggcggc cagcaaaatc agcggtgaag aagatcgcta
    44521 cagccacacc gatctgtggg atttccaggc taacgttgaa ggctcgcaga aaattgtcga
    44581 tctgctgcgt ccacaactac aaaaagcgaa tccggaactg ttggcaaaag tcgatgccaa
    44641 cttcaaaaag gtcgatacca ttctggcgaa ataccgcact aaagagggtt ttgaaaatta
    44701 cgacaaactg accgatgccg accggaacgc actgaaagga ccgattactg cgctggcgga
    44761 agatctggcg caacttcgtg gtgtgctggg gctggactaa gcgttatgca gtatgaagat
    44821 gaaaacggcg tgaatgaacc gtcacgccga cgtttactga aagggattgg tgcgctggcg
    44881 ctggcgggaa gttgtccggt tgctcatgca caaaaaacgc aaagtgcgcc gggtacgctt
    44941 tcaccggatg cacgcaatga gaaacagccg ttttatggtg agcatcaggc agggatcctg
    45001 acaccacaac aggctgcaat gatgctggtg gcgtttgatg tgcttgccag cgataaagcc
    45061 gatcttgaac ggttgtttcg cttgttgact cagcgttttg cttttctgac tcagggcgga
    45121 gcggcaccag aaacgccaaa tccgcgcctg ccaccactcg attccggcat tcttggcggc
    45181 tacattgcgc ccgataatct caccatcacg ttatcggtgg gtcactcatt gtttgatgag
    45241 cgctttggcc ttgcgccaca gatgccaaaa aagttgcaga agatgacgcg tttccccaac
    45301 gactcgctgg atgcggcgtt atgtcatggt gatgtgttgc tacaaatttg cgccaacacc
    45361 caggacacgg tgatccatgc gctgcgcgat atcatcaaac acacgccgga tttgctcagc
    45421 gtgcgctgga agcgggaagg gtttatttcc gaccacgcag cgcgtagtaa aggcaaagag
    45481 acgccgataa atttgctggg ctttaaagac ggcaccgcca atcccgatag ccagaatgcg
    45541 aagttgatgc aaaaagtggt gtgggtgacg gcagatcagc aggagcctgc gtggacaatc
    45601 ggtggcagct atcaggcagt acgcttgatt cagtttcgag tggaattttg ggacagaacg
    45661 ccgctgaaag agcagcagac gatatttggt cgtgataagc aaaccggtgc gccgctgggc
    45721 atgcagcatg agcatgatgt tcccgattac gctagcgatc cggaagggaa ggtgattgcg
    45781 ctggacagcc atatccggct ggcgaatccc cgcacggcgg agagcgagtc cagcctgatg
    45841 ctgcgtcgtg gctacagtta ttcgttgggc gtcaccaatt ccgggcaact cgatatgggg
    45901 ctactgtttg tctgctacca acacgatctg gaaaagggct tcctgacagt acaaaaaagg
    45961 ttgaatggcg aggcgctgga ggaatatgtc aaacctatcg gtggcggcta tttctttgcg
    46021 ctgccgggtg tgaaggacgc gaacgattat ctcggaagcg cattattgcg ggtttaatgt
    46081 ttttaggcgt ataaggcgtt tacgccgcac caggcgttcg tgcgctgatg cgacgcttgc
    46141 gcgtcttatc atgcctacaa tcggtgcggg tcggtaggcc ggataaggcg ttcacgccgc
    46201 atccggcaag cttttaccag taccgcgaat cctgctgaaa gcacacagct tttttcatca
    46261 ctgtcatcac tctgtcatct ttccagtaga aactaatgtc actgaaatgg tgttttatgg
    46321 ttaaatataa gtgaatatat tgttgcaatg aatgcgaaat ctgttgtact tattaagtag
    46381 cagcggaagt tcccggcagt gatagtcagt cactatggag atcgcggatg gtaacgtcct
    46441 gtactggaca tgttttagag aatcagcgcg ccaccactcg cggagatttc tcctccggta
    46501 gtcatctcgt cactttgtat tttcaacctc atcctttctt ttcatgtgtt accgacgccg
    46561 taaacggtgc gcgtagccgt ttttcggcat tttatccaaa agcaaactat ggcttacaag
    46621 gaagccaacc ctctgatgtt cgtgcgcata atcgcgctgc caacggcgcg tgtgatgaat
    46681 acaaacaact caaggtgctc tccatgggaa gacaaaaagc agtgatcaaa gctcgtcgcg
    46741 aggcaaaacg tgtgctgaga cgggattcgc gcagccataa gcagcgtgaa gaagaatcgg
    46801 tcacctcgct tgtgcaaatg agcggcgtag aagccattgg tatggcccgc gacagtcgcg
    46861 atacctcgcc catcctcgcg cgaaatgaag cgcaattgca ctatctgaag gctattgaga
    46921 gtaagcagct gatatttgcc acgggtgaag ccgggtgcgg aaaaacctgg atcagcgcag
    46981 caaaagcggc agaggccctg atacataagg atgttgacag gattatcgtc acccgtccag
    47041 ttctgcaagc cgatgaagat cttggtttct tacctggtga tatcgcagaa aagtttgctc
    47101 cctattttcg cccggtctac gacgtgctgg tccgtcgctt aggggcttcc tttatgcagt
    47161 actgcctgcg accggaaata gggaaggtgg aaattgcgcc gttcgcctat atgcgtggac
    47221 gtacctttga aaatgcagtc gtcattcttg acgaggcgca gaatgtgact gccgcgcaaa
    47281 tgaaaatgtt tttaacccgc ctcggggaga acgtgacggt tatcgtcaac ggtgatatca
    47341 cgcaatgcga tttgcctcgc ggcgtgcgct ccggattaag tgacgcgctg gaacgtttcg
    47401 aagaggatga aatggtcggg atcgtccgat tcggtaaaga ggattgcgta cgttcggcac
    47461 tttgccaacg tacgctacat gcatacagtt aagtgtatta tcggtgcaga gcccgggcga
    47521 accgggcttt gttttgggtg tttatacccg gactagcgtt ttttctgaaa ccaccatttt
    47581 tatttgtccc tggctggtga aatgtacgct catcctgtgg cttttttgta gttgctgata
    47641 aagctcatca ggtattgcta agctctctgc atattcttgc ggcgtatatt ggtatgctgc
    47701 atgatgctgc tgtttttgaa aacgcagctt attgtacaac gcccagacga ttaacacgac
    47761 ggcattcacc actgccagca aaaaatagaa ctgaagtcgg cttctggcct cgctttgcca
    47821 gaaataaccc gtcagcagat caatggcgaa taagaatatg aataaagcaa ataatgtcca
    47881 caagatagtt gtggcaacat aatcaaccag taaacgtact ggcgattgtc gggtcgtaat
    47941 aattagattg ttcatattaa cctctcagaa tcccacgatc gggacttacc caacgagcgc
    48001 gttgtttttt aggcatcaac atgactcgtg taaatgatac caacgtcgtt gccaggctta
    48061 gcatccagaa aataaccggg aaccaaataa tccagaaaag cgatgaagcc agattgtgct
    48121 catagcgatt ctcaatcacc agactgacaa taaattgcag taaacataac gtacacaata
    48181 atattccggc agtatgtgtc gctgcgatat gtgtcaattc aatatttaac ggtacacctg
    48241 caagttgggc tgcgtaaata atgaaaccga ccaggcaggt aaaggcccat attgtcgtca
    48301 gacagtattc aaaaaacagt ggccacattc gaaagttttc tttgcgccac aaccttgtca
    48361 tatttttgag gaatacttct gcaccgccct gagcccagcg caggcgctga ttccacagcc
    48421 cttttaacgt ttcaggcatt aatatccagc acagcgcccg tggctcgtaa aaaatcgtcc
    48481 actgattcaa ctgcagcttc cagctaatat caatatcttc ggtgatcata tcgtcactcc
    48541 agtaacccac ttctgccagg gcgctgcgac gaaatgcggc aataacaccg gaaacggtaa
    48601 atacgtttcc atagatacgc tgggtccgct tgatcaaacc aataattgag gaatactcgc
    48661 caacctgaat tttacccacc agggtagaac gtgtgcgaat acgaggatta ccggttacgg
    48721 cacccacacg cgggttgtac aacatcggtt ccacaatata tgccgctgca tcgcggtcta
    48781 ataacgcgtc gccatcaatg cacaccagat attcactttt cgccgcggca gctccggttt
    48841 taagcgcaat ggctttcccc tggttttgcg ccagatgaat gacccgcaga cggggaattt
    48901 gtgcagccat gcgatccagg atggcacggg ttttgtctgt tgaaccatca tttacggcaa
    48961 taacttcaat gttctcataa cgctgtgcta aagcggcatg tatggtttct tcaacgtttt
    49021 tctcctcatt aaaacaggga atgataatgg agatagatgg attatctttc aactgggggg
    49081 ctggtgcgtt ttctccccac ggccagtggc gttcacgata gacccagaaa tagacgccgc
    49141 caataatcca cataatggac ataaaaaacg gccagaagaa aacgaacctc atcatcagtt
    49201 caccagagtg aaagtacgct acgcataggg gtatgcataa caccagacat aatataaaaa
    49261 acgatacgat gcgattaatc atttttcgga taccaggctg ttgaaaactc aggacgaata
    49321 acgtctattt caggttggtt atgcagaaaa ttgtcgggat aataaccata atttttcact
    49381 ccattcagtt gtaataggct catccagtga gcgagttgtt gtgaagaaat agcctgatgc
    49441 tgaccatttt tctgccagtt ttgtgcctgt aattctaaaa ttgatttgtc tttagcctgc
    49501 gggatgcttt tcatctgatt ggtcaattgt attaaccatt gatcagccga tttttctgtg
    49561 acgccttcca tataaggcat agccataata gcggtccagt catagctttt taggaaatca
    49621 gcataattct gtgcaaacca ggcttcactt tcaggttgta ttaccggaag tgcaaaaata
    49681 tttcgtgcag ttttaacatg tggaccacga atggctttta cgcgcgcact aagttctaaa
    49741 gtgaagtcag ctaacgcacg acttttaaag cgggtccact gtttaaattg ctccgggttt
    49801 tgtcgaattt cgctcagact cccgctaaag ccagcttgct gataagccgc gatagccgct
    49861 gcactggcat cttcataatc tgaaagcaaa gcatcatcgt ggaacaatat gccatcaaaa
    49921 gcagcatgtc cggcaagatc atcatataac ataccaactt gtgctctgac cctgtcatcg
    49981 aaaggagaga gacggcggta ttgttcagga tgaatttgtg cttttttctc cccagttggt
    50041 aagtatttta ctcgcgttaa tgtgggatct aaatcccagc ttaataccgg catccacgca
    50101 tagatgttta cacctgagcg ggtacgcaat tgccaggcaa cccgactaaa aatatctgct
    50161 ttcattggta gcaaacgatt tggaaaccag acctctttaa ccagcccatc accatcggga
    50221 tcagcaaatg cttgcaaata cacggtagat atttgcatat cttttacccg ctgaattagc
    50281 acatcaatat tgcgatccat ttgctggcgg ttttcgtcat aaacgtaatc aagatcgata
    50341 tgcattaccc gttgtagtgg tttttcctgt acggtaataa tttgctgggc aaactctttt
    50401 aatgagggat tattggcgat taatactcgc ggaatggaat ctaattgcga cgcatttgcc
    50461 aaacctgatt caagggtgaa gaacatgtca taaccgagtt tttttaattc cgctattgct
    50521 atgccattcg cttcgccata aggccaaaca aaaacgtgtg gatttacctc agcctttgta
    50581 cgcaggtatt ccgtcatttt tacagcatcc aggcgaattc tttcccggta ttctgctgcg
    50641 gtttcatacc gtgcgtggtc agtaaaatat gcacgattta catatgcagg caataagctg
    50701 ccggtggcat tagcctgaat accgtagtga gaatcccatg tatgagaagc gacctcaacg
    50761 agccgggaac gcgcaacttc tcgcacttgt tgccacgtgg caaaatattc tcgactgacc
    50821 atctcatcgc caaatttcac ttgtttatcc gctggagtat cgacccaact gccgacggga
    50881 gcccatacag ccggccactg gaaggcctga agaattggga agacacgggt ataaaaactc
    50941 tggtagccgt catcaaaagt cagcactact gctttttccg gtagcggttt tcctcctcga
    51001 tgtgcttcac gaatttgagc aatactgacc ggttgataac cgttctcgcg cagccaggca
    51061 aattgttcac gcagtgctga tgttcgcact gacataaaac gctggtcggc agcttcgtct
    51121 tcaacgttat gccatgaaat caccacaaaa ccattatgtg gccacggttg ctcggcgagt
    51181 aaagattcgc gatcctgtgg cggtataaat gatgttcttg actggctaat gcacgcggtg
    51241 agcataatta tactcaccag catcaggaga tatttatttc cattacgtaa catatttatc
    51301 cttaaaatct gaatgtcata tcgaattcaa cgtataagtt gtgttctctg tcaccgtcat
    51361 aaggtcgttt ttcccagcgt agcgttgcgc cagcatcaat cacgtcattc cagctaatac
    51421 gttgcccgta gccgagttgg gtgacgacat ccgtgccata atgtttttgc caggaggcac
    51481 caacacctgc gctgaatatt tgctcccagc tattttcata gcttcgccat aacaaatgac
    51541 ttgcctcaaa tgccggaaca atatcgaaca tttttatagg gttgtagtat ggggtgtcgt
    51601 gttctgtatt ttgttcgtaa tacagattgg gtaggaaatc gacaatcaaa tatggtgaag
    51661 accagatgcg ttcttgaccc tcaattgaga cttcatgacg ctggttactg tcggaaaaat
    51721 cagtgaaagc ccaggagaca ccgtacttac gccgctcatt ttgataccag cgaacataag
    51781 cctgtgcgct gttgcctgta acaccatttt tcattgcccg taatggaacg cggtgagaga
    51841 ggcgttccag ttgcgaacca atacgccagt tatcattaaa atcataccag ccagacaggc
    51901 gcgcgccggg tttatgctca tgattgaaaa agcgttcagc gtactccgcc tcgagccaga
    51961 tattacgtga ccgccactca acacctgcca gccagtcgcg aacaatccct tttccttcgc
    52021 taaattgtcc atcggcataa ccgaatccag caaaaccgcg ccagttatca ttcagcggtg
    52081 gtgaataaac gatggtggtt aagtcgacat catgtttgcc actatctgga ccttcggcat
    52141 caattcctgt tgagccagcg atacgaagct ctgcaagatt atgtacatca accgcacgtt
    52201 ttaatcgtac aacgccggga tcttgcggtt cacgttcgac aacatcgtgg gttaagacag
    52261 ctgcctgctg ccattcttgt aacgttaatg ctgtccaggc ttgttcaacc tccagattaa
    52321 tattacgtgg ctcgatcact tctgcttttt ttaattcatt ttctgccgca cgaggccaac
    52381 cgcgggcttg taacacactc gcgtaatcga tgcgcagtcc ctgatttcct ggtgcgttat
    52441 aagcaagttc tctggttgtt atttcagctt gaggaagatc attactatat tttgctacgg
    52501 ttgagagaaa cgaatgcccc tgtaaccagg tatcattcgg gatgctcgta ggcgtgccca
    52561 ttagccgaag gaaaggcggc gaagtattaa tggtatgttt ggtgacagtt agcgcgccgg
    52621 gataattttc actctccagg tggctgtaaa agagatccgc aagttcttca tcggataaat
    52681 ccgtggcaat ggtttccttg ttataaaaga gctcggtcat tattgactgt gcttttttcg
    52741 gctgctgatc tttgagataa gccgacgcaa cccaatatag cgcccagggc ggaataattt
    52801 gccccgtctt ttttaaacgc tgatagtgag aaataacgtc tctataacga tcgcgagtta
    52861 ataacgcgcc aagatgatca acctgaatac gctggtactg ggcagtgcgg tctgggttat
    52921 cgtgccacag aatttctaat gcagcgtatt gggcgagggc gcgatcggca atggcataac
    52981 gttcattttc actgcgcgta ggcataaacg acagtctgac cagttcggca tgaatatcag
    53041 cgcgaatatc tggcgttaaa ttggcatcgt caatcgcggc agctagttga ttattacgta
    53101 atgccagcac gtattctgtg ggatattgtt gcttagatgc attttcaggt aatgactctg
    53161 tcatcgcccg taattcatcc tgataacgcc ccgtcagttt atagatatag gcttctgcga
    53221 gtaaattggc tttgtccggt gctccagatt taagctgctt aagtttaaac agcgcagtat
    53281 catagtgacc cgcatctgcc agggttaaaa tttgtccccg ttgataatcc ttattttgcg
    53341 gctccagaga gagcgccttt tgccacagtg taagcgagtt ttgccattgt tgcaggttac
    53401 gataagcgac ggcgacagct gcataaccac gcgctggtaa ttgctgatga cgatagcggt
    53461 tgtaaacggt aataacctgt ttatcttgcc cggcccataa ggcaatctgt aaccagtcag
    53521 caatttggtt attactgagt gttgattttt gtgcaaacca tgacaaagct ggctgagtat
    53581 ttcctttgcg agcttcaata atcaatgcat cataggcgtt attaacagca ctcttcgccg
    53641 cgggactcgc tgctaaaaat gcagcagtaa gaattttcaa agcccatttg gttttcgggc
    53701 acctttttct gctacttgaa tacatcctgt attactccat gtattgccaa aatctctctc
    53761 tgtatctaat tacaggtaac tgaaaagaaa gatatttttg catctcataa tccgttatta
    53821 aacgcggaag agagacgtga attgttgatg atgagaagaa gaaatgatga gcagagtgtc
    53881 catataaaat ccttttctcg cccgaaaatc cattccaatg atgaggatct tcaggaatac
    53941 ggcataaatc ccaatacctt tttcaaaata aattaggatt aaaataatta aatcagtaaa
    54001 ttccgatgca tgatttcgga tttttcgaaa gtcctgatga aaggcagcgc tactgcactc
    54061 ac
//
LOCUS       DAPQZD010000012        52445 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPQZD010000012 DAPQZD010000000
VERSION     DAPQZD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.012"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..907)
                     /gene="wcaM"
                     /locus_tag="RO454_000739"
     CDS             complement(<1..907)
                     /gene="wcaM"
                     /locus_tag="RO454_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="WcaM from Salmonella enterica is essential for
                     biofilm formation on HEp-2 cells and chicken intestinal
                     tissue but not on a plastic surface; may be involved in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDY0932616.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDN"
     gene            complement(918..2138)
                     /gene="wcaL"
                     /locus_tag="RO454_000740"
     CDS             complement(918..2138)
                     /gene="wcaL"
                     /locus_tag="RO454_000740"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDY0932617.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATSLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(2135..3415)
                     /gene="wcaK"
                     /locus_tag="RO454_000741"
     CDS             complement(2135..3415)
                     /gene="wcaK"
                     /locus_tag="RO454_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDY0932618.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDKQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(3884..5362)
                     /gene="wzxC"
                     /locus_tag="RO454_000742"
     CDS             complement(3884..5362)
                     /gene="wzxC"
                     /locus_tag="RO454_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDY0932619.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(5364..6758)
                     /gene="wcaJ"
                     /locus_tag="RO454_000743"
     CDS             complement(5364..6758)
                     /gene="wcaJ"
                     /locus_tag="RO454_000743"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDY0932620.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(6814..8184)
                     /gene="cpsG"
                     /locus_tag="RO454_000744"
                     /gene_synonym="manB"
     CDS             complement(6814..8184)
                     /gene="cpsG"
                     /locus_tag="RO454_000744"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDY0932621.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(8289..9725)
                     /gene="cpsB"
                     /locus_tag="RO454_000745"
     CDS             complement(8289..9725)
                     /gene="cpsB"
                     /locus_tag="RO454_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDY0932622.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(9728..10951)
                     /gene="wcaI"
                     /locus_tag="RO454_000746"
     CDS             complement(9728..10951)
                     /gene="wcaI"
                     /locus_tag="RO454_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDY0932623.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAAMVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(10948..11427)
                     /gene="gmm"
                     /locus_tag="RO454_000747"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(10948..11427)
                     /gene="gmm"
                     /locus_tag="RO454_000747"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDY0932624.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGSDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(11430..12395)
                     /gene="fcl"
                     /locus_tag="RO454_000748"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(11430..12395)
                     /gene="fcl"
                     /locus_tag="RO454_000748"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDY0932625.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(12398..13519)
                     /gene="gmd"
                     /locus_tag="RO454_000749"
     CDS             complement(12398..13519)
                     /gene="gmd"
                     /locus_tag="RO454_000749"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDY0932626.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(13546..14094)
                     /gene="wcaF"
                     /locus_tag="RO454_000750"
     CDS             complement(13546..14094)
                     /gene="wcaF"
                     /locus_tag="RO454_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDY0932627.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            complement(14110..14856)
                     /gene="wcaE"
                     /locus_tag="RO454_000751"
     CDS             complement(14110..14856)
                     /gene="wcaE"
                     /locus_tag="RO454_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDY0932628.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLHFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(14937..16157)
                     /gene="wcaD"
                     /locus_tag="RO454_000752"
     CDS             complement(14937..16157)
                     /gene="wcaD"
                     /locus_tag="RO454_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDY0932629.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(16132..17349)
                     /gene="wcaC"
                     /locus_tag="RO454_000753"
     CDS             complement(16132..17349)
                     /gene="wcaC"
                     /locus_tag="RO454_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDY0932630.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(17346..17834)
                     /gene="wcaB"
                     /locus_tag="RO454_000754"
     CDS             complement(17346..17834)
                     /gene="wcaB"
                     /locus_tag="RO454_000754"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDY0932631.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(17837..18676)
                     /gene="wcaA"
                     /locus_tag="RO454_000755"
     CDS             complement(17837..18676)
                     /gene="wcaA"
                     /locus_tag="RO454_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDY0932632.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(18805..20967)
                     /gene="wzc"
                     /locus_tag="RO454_000756"
     CDS             complement(18805..20967)
                     /gene="wzc"
                     /locus_tag="RO454_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDY0932633.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLTGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKVLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(20970..21413)
                     /gene="wzb"
                     /locus_tag="RO454_000757"
     CDS             complement(20970..21413)
                     /gene="wzb"
                     /locus_tag="RO454_000757"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDY0932634.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYALLERSAHQWAQALNAEQV"
     gene            complement(21419..22558)
                     /gene="wza"
                     /locus_tag="RO454_000758"
     CDS             complement(21419..22558)
                     /gene="wza"
                     /locus_tag="RO454_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDY0932635.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTLGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            22868..23017
                     /locus_tag="RO454_000759"
     CDS             22868..23017
                     /locus_tag="RO454_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932636.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCRSIWFL
                     GVSLD"
     gene            23218..24801
                     /locus_tag="RO454_000760"
     CDS             23218..24801
                     /locus_tag="RO454_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDY0932637.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(25253..27106)
                     /gene="asmA"
                     /locus_tag="RO454_000761"
     CDS             complement(25253..27106)
                     /gene="asmA"
                     /locus_tag="RO454_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDY0932638.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(27128..27709)
                     /gene="dcd"
                     /locus_tag="RO454_000762"
     CDS             complement(27128..27709)
                     /gene="dcd"
                     /locus_tag="RO454_000762"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDY0932639.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(27801..28442)
                     /gene="udk"
                     /locus_tag="RO454_000763"
     CDS             complement(27801..28442)
                     /gene="udk"
                     /locus_tag="RO454_000763"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDY0932640.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            28760..32077
                     /locus_tag="RO454_000764"
     CDS             28760..32077
                     /locus_tag="RO454_000764"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDY0932641.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     SPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(32184..33032)
                     /gene="alkA"
                     /locus_tag="RO454_000765"
     CDS             complement(32184..33032)
                     /gene="alkA"
                     /locus_tag="RO454_000765"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDY0932642.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSVGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            33166..34518
                     /gene="yegD"
                     /locus_tag="RO454_000766"
     CDS             33166..34518
                     /gene="yegD"
                     /locus_tag="RO454_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDY0932643.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(34531..36471)
                     /gene="yegI"
                     /locus_tag="RO454_000767"
     CDS             complement(34531..36471)
                     /gene="yegI"
                     /locus_tag="RO454_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDY0932644.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTS
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPSGLLRREYI
                     ILIEIALSGLSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKVEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(36468..37229)
                     /gene="pphC"
                     /locus_tag="RO454_000768"
     CDS             complement(36468..37229)
                     /gene="pphC"
                     /locus_tag="RO454_000768"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDY0932645.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQIQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLALNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(37226..37885)
                     /locus_tag="RO454_000769"
     CDS             complement(37226..37885)
                     /locus_tag="RO454_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDY0932646.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGSAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(38103..38159)
                     /locus_tag="RO454_000770"
     CDS             complement(38103..38159)
                     /locus_tag="RO454_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDY0932647.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(38431..38487)
                     /locus_tag="RO454_000771"
     CDS             complement(38431..38487)
                     /locus_tag="RO454_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDY0932648.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            38766..40013
                     /gene="mdtA"
                     /locus_tag="RO454_000772"
     CDS             38766..40013
                     /gene="mdtA"
                     /locus_tag="RO454_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDY0932649.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKAASREDTKKGARS"
     gene            40013..43135
                     /gene="mdtB"
                     /locus_tag="RO454_000773"
     CDS             40013..43135
                     /gene="mdtB"
                     /locus_tag="RO454_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDY0932650.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            43136..46213
                     /gene="mdtC"
                     /locus_tag="RO454_000774"
     CDS             43136..46213
                     /gene="mdtC"
                     /locus_tag="RO454_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDY0932651.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            46214..47629
                     /locus_tag="RO454_000775"
     CDS             46214..47629
                     /locus_tag="RO454_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0932652.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            47626..49029
                     /gene="baeS"
                     /locus_tag="RO454_000776"
     CDS             47626..49029
                     /gene="baeS"
                     /locus_tag="RO454_000776"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDY0932653.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            49026..49748
                     /gene="baeR"
                     /locus_tag="RO454_000777"
     CDS             49026..49748
                     /gene="baeR"
                     /locus_tag="RO454_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDY0932654.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            49928..50260
                     /locus_tag="RO454_000778"
     CDS             49928..50260
                     /locus_tag="RO454_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDY0932655.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            50407..51768
                     /gene="yegQ"
                     /locus_tag="RO454_000779"
     CDS             50407..51768
                     /gene="yegQ"
                     /locus_tag="RO454_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDY0932656.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT        12115 a        14202 c        13563 g        12565 t
ORIGIN      
        1 agttgtcaca accataaata gcaactgtgg cgttatcaat acctgctttc ttactgaaat
       61 ccggcgtgat attacgagct ttgatattac ggataacaaa atgtttacca ttttcaacat
      121 ggatcagttg ccgacaatcc gatcccgtga tattcgccac gacaaagttt ttcactgcct
      181 gatcttctgg gtaattatta tcataagtgc ttcccgcaag gcctatgcca atgccccagt
      241 tgattttgcc attagtacag ttgatgcgct cgatgacatg gtcggatatc aaaatatcac
      301 tgtcgttaat cgccacgttc cattcaatag cgtcgccctg taagtcgctg aacttacagt
      361 tggtgatgtt ggcaccgatt atctggttat gaaatccctg gcgtaagatg gcgtaattag
      421 cgtggctaac ggtcaggtta tcgatggtca ggttgtgcat gacccgtttg tttttgccgc
      481 cgatataaat ctgcgttacc ggaccaaagc cgctcatcgc cagccctttg atggtgcagt
      541 cagagccgcg cacatccagg gtgatgttat gcatactgcc gcccccctcc cctgtcacct
      601 ggctgccgtc ctgtaagaca aatcgccctt tgccgttgcc gcgcaggctt ccaaggatgt
      661 gtaacgtttt gccaggagga atgaagatgc cggtgttgat attgtcacaa accaatccgg
      721 cgggcacgac gaccgtttgc ccttcgctga aggcttgttt aaatgaggcg atccagtcgt
      781 gagggttgta gtcgttaatg ttaacgcttt gttgagcggg aagtgcgcgg gcgaaaggcg
      841 tatggaggaa ggcaagcaca gaacttgccg tcaggaaggt gcgtcgggag agttttttaa
      901 atggcatgcg ttctcctcta taaagcctgc agcaagctgg cgagttctcg attgatcacc
      961 tgctggttaa aatcgtgttc gactttttcg cgagcacgtt tgacgacagg agccagttcg
     1021 tcgctgtcca gttggctaaa cgccgccagt cgttgcgcca gtgcgcgagc atcgttctca
     1081 ggcaccagcc agccggattt gtcagcctcc accagttcag gaattccgct atgcagagta
     1141 gaaaccaccg gaataccgac cgccatcgct tccattagcg ccaccggaat accttccata
     1201 tcgccatccg cacccgtaac cgatggcaac aggaagacat ccgcgtcatc gagcatctct
     1261 ttcacttcat ggctcggttt aaagcccggc atctccacca catcttccag ttgatattgt
     1321 tcgatgaggg tgcgcaggcg tcgttcccac gggccaatgc cgaggatgcg atagcgaaat
     1381 gccacgccct gctctttcaa ctgacggcag gcttcaatcg ccacatgcag gccttttttc
     1441 tcggttaagc gtgcgacgga aatgatttcc agcggcgttg caggcgcttt caccggacgc
     1501 gggctaaagc gcgtcatgtc cacgcccatg cgcgatacgg cgattttttc cctcgggcag
     1561 cccatttttt gcagccttcc ggcccacaga tcgcttattg gtagcatcag gtcgccacgg
     1621 cgaaacagtt gctgatattc gggagtgtaa tggttgagca cctcccgact ggagatatca
     1681 atgccgtgga agatagtggc aattttgccg cgaatgacac ccagttcgcg gagttttgct
     1741 gcggttaccc ccgcagggcc aaaatgtgcg ataaagacat cggcatgtaa cgatgttgcg
     1801 acctgaccac aaatggcaga caaaatcagg ttccgcgact ctgcgccata gcgtttgagg
     1861 ttgagcgcct gccaggtatt tttacgatga atgccgcgta aggtctggct ggctcggtgg
     1921 cgcagcttcg ccactttgcc cgtaggttcg tcctgtaacc agcgggttct ggcggcaagg
     1981 ttgtatttcg tccatgccgc gtgggtgttt tgtgtgtcgc ctttttgcaa cgcgacaatc
     2041 tccacctcaa agcccatatc aataaacgca gtgatttggt tgaggacgaa ggtttccgac
     2101 gacagcggaa atttcaataa aaagaagccg accttcattt cacctccccg atgcgctcaa
     2161 gcacagactg caccatctgc attcctgtct gacgttcgcg actgacggct tcgttaagtc
     2221 gtgcgttcag cgccggaagt tggcctaagg tatccgcaac catcgcttgc aggctgccgt
     2281 ctaataaatg acggatatca atcgccatct ccggtagtcc cagctgctgc atgatcccgg
     2341 cggatttatg ttcatagttg atggcaatcg ccggggtggc aaagttcatt gagataatgg
     2401 cggagtgcag gcgcgtaccg acggtgagtt cacaggcccc cagaattttg cccatttcca
     2461 gatcgttgag ttcatccatc accacgtggt aacgggcagg atcgctgatg tgctggcgca
     2521 gattgagcgc caccatgcgg tcgtctttgt tatagctgtc gatgcccgta caggtggaga
     2581 gcgcaatcac ctgatagcct tcgtcgagaa tgcgattgac caccccggca aaggcttttt
     2641 catacgcttg ttgagtggtg ccgaggcgtt tgtcgaacgg tgccagttca cgcagggtaa
     2701 tggccaccgc tttctgttgt gcggcaacgt ccagccagtg ttgaacagca tagctggcgg
     2761 tgaagtcttc tgtgtggtga tcgaccagcc acgcggtatc gacgccatgt tcaacttttg
     2821 cggtagtgat attgctgcgt ttcatcaaat caaggctgac cgattcgcgc aggatcagcg
     2881 cgtcgcagtg accaaaaacg tagttcgcca gttggttaaa ttgcttatcc tggaacgggc
     2941 cgacgctgtg accaatcata aacagcggct ttttcgccat aaacgtgcaa agcgcatgtt
     3001 caaactgcgg cacgccatag agatcgacaa aaaacgatcc gccgacctga ataatggcgt
     3061 cgtaacctga cagcaggcgc acgaagtcag tgaatccctg ggcgatggcg atattacgca
     3121 acttgccagt gtcggtgacg cgtgagagca gtacctgatg ttggtaacgg cgacggagga
     3181 cttttttaac gcgcccgaca acgcccgctg cactgttgtg ttgtttcatt tgcaggaaca
     3241 gcggatcgcc cattaccggg cggttgagca gccaggaaga actgaccgga tagcgactca
     3301 tcacgtccac ttcggcatgt ggattgagga tattgatggc atcaagtaag ccgcgcagga
     3361 tggcgctgtc gccgcgattg ccgcaagtgt ggttgcccag aataagtaat ttcatttttt
     3421 cctcataaat ttgatgccag gtgaggctgt gtttgttgtt ttcattgctg ttttttgcct
     3481 gatgcgacgc tgacgcgtct tatcaggcct acaagatccg agcacagaac cgtaggacgg
     3541 ataaggcgtt cacgccgcat ccggcattca gtgcctgatg cgacgctgac gcgtcttatc
     3601 aggcctacaa gacccgagca cagagccgta ggacggataa ggcgttttac gccgcatccg
     3661 gcattcagtg cctgatgcga cgctgacgcg tcttatcagg cctacaagat ccgagcacag
     3721 aaccgtaggc cggataaggc gttcacgccg catccggcat tcagtgcctg atgctacgct
     3781 aacgcgtctt atcagaccta caagatccaa gcccagcccc ccaggacgga taaggcgttt
     3841 tacgccgcat ccggcaaccg ttgccggaac cgaaaacaac cattcacccc gcccgtaaaa
     3901 gcattttcat tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc
     3961 gcgacagcac aatcatcacc acaaacgcca gcacgcccgt ggctatttgc accgccagca
     4021 gcatccccag cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg
     4081 tcggcagcga gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac
     4141 cgagcaccgg tttaatcatc acgaaatagc tcagaatggt gttgataatc tgcaccagca
     4201 ggaagccaag cgtgacgccg attgcgcccg ccatctgccc accaatgatg attgccggaa
     4261 taaacagaaa ggttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca
     4321 tcagcagcga accaatcggg ttacccacgg agcgcagtag ccccacaata cacagcaatt
     4381 gcagcaccgg aataatgctg ctccactttt cgccaaatac cagcggtaca aagttattcg
     4441 acaccaccat tagccccagc agcgccggaa agttgataat ccccaccacc gacagcagct
     4501 tgtagaagtt gacgcgcagc ttttctgtat cgtcctgaat tttggcgaac gccggaaaca
     4561 acacgcgggt gatgatcggg ttcagcttca tcggtggcac aacggccacg ttgtacgcca
     4621 ggttgtatcc cccagccacg cccgcgccga gaatacgcgc cagcaccagc gttgaaaggt
     4681 tggtgttgag atagttgatg atgctgtccg ccgtcagcca ggcaccaaag cgtaagttcg
     4741 gtgccaccga cgccagcgag aaatgcagac cggggcgata aattttgcgg ccaaagtagc
     4801 caaacagcag cgttctcacc gcactattga ccagataacc gagaatcgcg gtcatcgcca
     4861 gcggccagaa atgggcgcta accaccgtaa aagtaaagcc cgccagcacc gcgctggttt
     4921 cgatcatgcc gattttgttg aactccagct ccttttgcat caacgcgcgg aactgttgcc
     4981 cgtgggggat caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt
     5041 tattcagcaa gtcgccgatg agatcactca acaaaaacac cgccacgcac accacgatcc
     5101 ccagcccgac gttcagccag tacagcgtgg tgagttcaag gtggctgatt tcttttcgct
     5161 gaataatgga gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg atgatcacca
     5221 gcgacacggt aagcaaaccg aactggtggt tatctataat ccgcgccagc acagtcattt
     5281 gcaccagacc gaggccgatg atgatcaccg tggcaatcgc cgaccacttc gcgccgctga
     5341 tggttttttc acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc
     5401 gtcaggaaaa cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg
     5461 aactcgacgc gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccgttaatc
     5521 tgcgcccagc cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc
     5581 tggcgatact gttcgttatg cgccaccgcg tgcggacgcg gaccgacaat cgacatcccg
     5641 ccagtcagca cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc
     5701 cccactttgg tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc
     5761 atcactttca tggaacggaa cttccacact ttgatcggct tgccatccat gccgtagcga
     5821 gtctggcgga aaataaccgg ccccggtgag ctcagtttca ccgccagcgc aatgcagcac
     5881 agcaccgggg agatcagcag caggataagc gtcgccagca caatatcttc cgcacgtttt
     5941 atcaggcggt taaccccgga aagcggcgtg tcatacagcg gcaccaccgg tacgccgttc
     6001 atctcttcga tacgggaatg gagaatgttg aaggtaaaga catcggggat cagcagcacc
     6061 gaacaggtgg tgtccgccag ttgatggacc agttttttca ctcgcgcgcc gtcgcacatc
     6121 tgcatcgcga tatagacgtt atgaatcttg cccgctttcg catcctcgac cagctgttgc
     6181 agattacccg cccagtcgtt agaaacgcca cccggtttcg ggtcgtggta aacgcccacc
     6241 acttcaaacc ctaaccacgg ctgattacgg aagctctcca tcagcatttg tccggcggct
     6301 aaatcccccg ccaccgcgac catgcgcttg ttatagccat gattacgcag ccagcccgcc
     6361 ccaatacgaa tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc
     6421 agccagattt tcaattgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa
     6481 atcacgctta acgtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg
     6541 cgccatgagc gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc
     6601 agcgccacca acaggtgcat gtagaggaat gacagtccgc tcacttcgca aaccagccat
     6661 agcccggcaa acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca
     6721 ttggttttcg ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt ttttagcccc
     6781 ttacccgcag tgggggtaag ggaagatccg acattactcg ttcagcaacg tcagcagagt
     6841 tcgcgttcgc gcttccatca gcggcacatc accgcgtgat tccacattca accgcaccac
     6901 cggttcggtg ttggaggagc gcagattaaa gcgccagtcg gcaaaggtca tgctgatgcc
     6961 atcggtacga tccaccgcca gtgcctcacg gctaaaatgc tgttccacgc ggttaatcgc
     7021 ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt gccgggaatg ccgccatccg
     7081 gtcgcgcacc agttcgccca gcgtttttcc tttcagacac accagttcgg cgaccagcag
     7141 ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg aagtaatggt gggcgctcat
     7201 ttcgccaccg tagatggcgt cttccttgcg catacgttct ttaataaagg cgtgtccggt
     7261 tttcgacatt accggggtgc cgcctgcggc agtcaccaca tcaacggtgt tccaggagag
     7321 acgtggatcg tggatgatct tcgcgccggg atttttttcg aggaacgctt ctgccagcaa
     7381 accgacaatg tagtaaccct cgataaactg ccctttttcg tcaaacagga aacagcggtc
     7441 aaaatcgcca tcaaaagcaa tgcccatatc cgcgccgtgt ttaatgaccg cattgcgggt
     7501 gtcgtcgcga cattccggca gtagcgggtt aggaataccg ttggggaaat tgccgtccgg
     7561 cgtgttatgc actttgatta actccaccgg tgcgccgagg gctttaaagc gggcttcaat
     7621 ggcgtccacc accggacccg ccgcgccgtt cccggagtta atcaccagct tgagcggcgt
     7681 gaggtttttg acgttgatat aaccgaacag gtgatcaacg taagcgtcac gcagattgat
     7741 ttgctgatag cgaccgcgtt tggtttcatc gacgggagga aagtcgttgg cttctgccag
     7801 acgctggacg tcgcgcagtc cggtatcgcc gctgatcggg cgagcccctt cgcgcaccag
     7861 cttcatgccg ttgtaatcca tcgggttatg gctggcggta acttcaatgc cgccatccac
     7921 gccgagatgg aacgtggcga aatagatttc ttcggtgccg gacatgccga tatccagcac
     7981 gtcgacgccc gcatcctgta aacctttcgc cagcgccagt tttaaggttt cgctggtgag
     8041 gcggacatca ccgcctaaca caatggtttt cggtttgaga aattcgccat aggcgcgacc
     8101 aatgcgccag gcgatatctt cattcagttc ttcgcctaat ttcccgcgaa tatcataggc
     8161 tttaaagcag gttaattttt tcatatcgtt accctttttc aggcaatagt tggccctgac
     8221 cgaaacggcc aattttgttt ttgtcgttat ttacatcgcg gtttcgcatt cattgcctga
     8281 tgcgacgttt acacccgtcc gtagcgatcc gcgaagcgca ccacatcatc ctcttcgaga
     8341 taagagccgg agcgcacttc aattaaatcg agcggaattt tccccgggtt ttccaggcaa
     8401 tgcgtcgccc ccagcggaat ataaatggac tcgttttcac caagcagttt gatatcaccg
     8461 tcgatagtga cttttgccgt tcccgcgaca accacccagt gttccgcgcg gtgatgatgc
     8521 atctgtaccg acaacccttc gcccggtttc acggtgatgc gtttcacctg gtagcggtcg
     8581 cccgcgtcga tagagtcata tttgccccac ggacgataca cttcgcgatg cacccgatgc
     8641 tcatggcgac catcagcttt gatctgctcg accacttttt tcacatcctg caccgcgtta
     8701 cggtcagcaa tcagcactgc atctttggtc tgcactacca ccaaatcttt caccccgacg
     8761 gtggtgacca ggccagattc ggcatacaca tagctgtttt cagttttgtg attaatcaca
     8821 tcgccatggc aaacgttgcc ctcggcggtg tgggcactga tctcccataa agaggaccag
     8881 gaaccgacat cactccagcc cgcatccatc ggcaccacaa cggcatctgc cgtgcgttcc
     8941 atgaccgcgt aatctaccga ctcttccgga caggcgagaa acgcctcttc atccacacga
     9001 ataaaatcga gatccggatc gacggcgctc atcgcttttt cacaggcatc gagaatatcc
     9061 ggacgatact ttttcagttc ttcgagataa cgtccggcac ggaacaggaa cataccgctg
     9121 ttccagtaat attcgccact tgccacatag gcctgcgcgg tttccagatt cggtttttcg
     9181 acaaactgcg ccacttcaaa ggccaccgta tcctgctcac ccgccgacac ttcaccgcga
     9241 cgaatatagc catatccggt ttcaggtaga tccggcacaa tgccgaaggt caccagcttg
     9301 cccgcttcgg catacggcat ggcattacgc acggcggcac ggaacgcgtc ttcatcggca
     9361 atcacatgat ccgccgccag caccagcatt aatgggtcgt tctccgggct atgacgtttt
     9421 gccgccagcg ccgccagcgc aatggcaggt gcggtgttac gccccgccgg ttcgagaata
     9481 atgttctcgg tgagtttatt tagttgacgc agctgttccg cgacaataaa gcggtgctgc
     9541 tcattgcaaa tcaccaccgg gctttcgcac tccacgccgt tcaggcggca gatggtggtt
     9601 tgcagcatgg tgagatcgcc tttcaggcat aaaaactgct tggggtaaag tacgcgggaa
     9661 agcggccata agcggctacc ggagccacct gccatcacaa ctggatagag tttcgactgc
     9721 gccataatta tccccgaata tcatttataa attgacgtaa cacgttctct ttatcgagcg
     9781 tgcgttcggc atattcacgt gccaccgtgt tgtgtttggg cagcaggagc gcctgacgaa
     9841 tccccgccac cagcgcctcg accgattccg gttccacgca aaccgcaatg cccggaaagg
     9901 tttcgcaaag ctgccccagt tcggtgtggg cttctgcggt aatcaccgcg ttaccgccta
     9961 ccgccagaat attggtcagt ttcgacggca atacggcatc tgccgcgccg cgtttttgca
    10021 ccaccagatg gcaatccccc atcttcagca gtgcgggtaa agcgtcatac gattgcagcg
    10081 gaaaaaattg catgttgcgc agtccacgct gctgcgccat tttttccagc cgcgctttgc
    10141 cgccgccctg cccgacaatg gcaaaaataa gcggttcatc gcgcaggcga tcggcagctt
    10201 caataacgtt ttccagcccc tgcttttcac caatattgcc ggagtaaaga atgatttttt
    10261 tgttatccgg caggccaagc tggttacgaa gggcatcaac atcggcgtct gcgacatgct
    10321 gaaaacgggc gatttccgac cagtttggga agaaaatgac gttttccgcc gccacgcctt
    10381 tttcgatggc tttattcatc atcgaacgcg aaatcgtgga gacgttatcg acgttatgca
    10441 gtccgctacg ttcgaacgcc gtcgccagct gtgccacttt gccgcctttg ccttttccgg
    10501 caaggcccag ccccagcatg gcgtccactt cgtaatcctg aatatgcagc acggtacgcg
    10561 caccagagag tttcgccagc aggcgcattc ccggcgtgca aaacagcgtt ggcactacgc
    10621 cgataatgcg atccggcttc cagcgacgtt gcgccatcag tggaaagaaa ctgctgacgg
    10681 caaaactgcc gagatgcaac aggcgtttca gagtgctcgg ctgttttggc acgtacagcg
    10741 ggcagcgcca caccatggcg gccccctctt cccgtttgta ccgccaggcg gaatagttct
    10801 cgcccacctg ccactgcggg tagtaaggcg gcgcggtaat gacccgcacc tcatgacctt
    10861 gtgccgccag ccattccacc atctcgccgg tgtatttgcc gatgccggtt aactccggcg
    10921 agtagttaat gccgtagacc agtattttca taatccgggt actccggcac gcttctcagc
    10981 gagaaaatag gcgcggctgt tagcgtgaac attgtcactg gcgagcaacg cgtccggcgt
    11041 cagccagcgg taatcgtcat gctgctcatc cggcagtaac agctcttcct ccgctactct
    11101 gaagcgaaaa ccgagcacca catagtgagt ggtaaaatcc gagccggaga agttatcgtc
    11161 ataaaagtgc tgccagacac cgtaaaactg gcctgctgtt atcggcagac gcaaccccag
    11221 ttccgccatc gtcagccgct caaatgcggc ttccagcgtt tcgtctttct gcacgcgccc
    11281 tcccggcaca aaccagtaac cctgcgccgg gcggttggtt cttttgccaa gcagaaactc
    11341 gccgcgactg ttctcgacaa taaagtcgag agagacaagc ggagtggagc gcactaccgt
    11401 ggcaaagtct tcctgacgta aaaacatcat tacccccgaa agcggtcttg attctcaagg
    11461 aaccactggt aagtgctggc aagccccgct tccagtgaga tttcgtgata ccagccaagc
    11521 tgatgcagcc gcgtcacatc cagcagtttg cgcggcgtgc catccggttt gctggcatca
    11581 aaaaccaccc gacctttgta acccaccact ttggcgatgg tttgcgccag ctcacggata
    11641 gtgcagtcaa cgcccgtgcc gacgttaatg tgcgacagca tcggctgggt gttctcctgc
    11701 cagacttcgt gtgccagctc catgacatga atgctcgccg ctgccatatc atcaacgtgt
    11761 agaaattcgc gcatcggcgt accgctgccc cacaccacca cgtccggcgc attctgtgcc
    11821 gtcgcctcat ggaagcgacg cagcaatgct gggatcacat gcgaattact cgggtggaag
    11881 ttgtcgtgtg gcccgtacag gttggttggc atgactgagc ggtaatcccg tccgtactgg
    11941 cgattgtaag attcacacag tttgatcccg gcgattttgg caatggcgta aggctcgttg
    12001 gtcggctcca gcgtgccctg caacaactcg ctttctgcca tcggctgttt tgccagtttc
    12061 gggtagatgc aggacgatcc gagaaacagc agtttgttca cgtcgttctg atgcgcggcg
    12121 tgaatgatgt tgctctcaat catcatgttc tggtagatga aatctgccgg atacgtgttg
    12181 ttggcaacaa taccacccac tttcgccgcc gccagataga cctggtcaat gctttcgctg
    12241 gcaaagaaat catgcaccgc gcggctgtcc agcaggttca gttcatcgcg ggtgcgtaat
    12301 accagctcca catcaccgcg ctgttcgagc tgccgcctga tggcggaacc gaccatcccg
    12361 cgatggccag caataaaaat gcgttgttta ctcatgctta tgactccagc gcgatcgcca
    12421 cgtcgtagcc gtgagatttc agcagagagt gttttttcgc cgcttcaagg tcattagcca
    12481 ccatttcaga caccatctct ctgagggtga tttccggttt ccagcccagt ttttcgtgcg
    12541 ctttggtcgg gtcaccgagc agcgtttcaa cttctgccgg acggaagtag cgcgggtcaa
    12601 cggcgataat cacatcaccc ggtttaacgc ccggcgcgtc atgcccggtg acggaaacca
    12661 caatgccctt ctcttcaaca cccgtgcctt caaagcgcag tttgatgccc agttgtgccg
    12721 ccgccatttc cacgaactga cgcacggagt actgaacgcc ggtggcaata acgaaatctt
    12781 ccggttgttc ctgttgcagc atcatccact gcatttttac gtagtctttg gcgtggcccc
    12841 agtcacgcag ggaatccata ttgccgagat acaggcacga ctccagcccc tgggcgatgt
    12901 tggcgattgc gcgggtgatt ttgcgggtaa cgaaagtctc gccacggcgc ggggattcat
    12961 ggttgaagag aataccgtta caggcgtaca tgccgtagga ttcgcggtag ttaacggtga
    13021 tccagtaggc gtacagtttg gcaaccgcat acggagatcg cgggtagaac ggcgtggtct
    13081 ctttctgcgg aatttcctgc accagaccgt acagttcaga agtagaagcc tgatagaaac
    13141 gggttttctt ttccagaccg aggaagcgga tcgcctcgag caggcgcagc gtacccatcg
    13201 catcgacgtc tgcggtatat tccggtgact caaaagaaac cgcaacgtgg ctcattgccc
    13261 ccaggttgta cacttcatcc ggctgcactt cacgcaaaat gcgcgtcagg ttggaggtat
    13321 cactcaggtc gccataatgc agatggaatt tcgggttgca ggtgtgcgga tcctgataaa
    13381 tgtgatccac gcgctcggtg ttgaatgacg atgcacgacg cttaatacca tgcacctcgt
    13441 aacctttttc cagcagaaac tctgccaggt aagaaccgtc ttgtccggtt acaccggtga
    13501 tgagagcgac ttttgacatg tcttattcct ctgtattttt tgaatttatt cagtttcaac
    13561 gcgtttgcgt atcaccactg cggggtgccc ccgacaaacc acatttgccg gaagcgattt
    13621 aaaaacactg cttcgcgcac ccacgacggt gccgtcgccg atcgtgacgc caggggcaac
    13681 aaagacatcg gttgccagcc agcatttctc gccaatcaca ataggcgtgg cgttaatggt
    13741 gaaatgttga cttgcatggt cgtggctacc agtgcataaa taactttttt gcgatatcac
    13801 cgaatgtgcg ccaatggtta tttcaccgag ggtatataaa ttgacctcat cgccgaccca
    13861 cgcgtaatca cctaacgtta atttccacgg ataggtaatt tttactgacg gacgaatgac
    13921 tacgtttttt cctatttttg cgccgaataa acgtaataaa aatgcccgcc agcgatacaa
    13981 tacttgtggc gaccaggcaa atattgttgc ctgtactgcc caccacaatt gcactttaat
    14041 agcgttgcca ccccggaacc ctttcggcac cgagaatcca cttaaatcct gcatcgtttt
    14101 cccttatatt cagcttttgt tatataaggc tttcgtcttt gaggtcgtac gttggcgtaa
    14161 atgccaggat aattcagccc aaaagccagg cacgtgcaat atttgccgtt ggactttttt
    14221 ggcgtcagca cacaattcca tattattggt ggtagatacc ccacccatag aaaattcaga
    14281 caccaggcca ttgagttttt taaatgcata accggcttta tacattttgg ccgccagcgc
    14341 gtaatcggaa gaaactttat actccaggtc ataacgccat ttcttcaagc cggacaccgg
    14401 gaaaaatatc gcctgatgac tggcgggcag gctgtggtaa atataccaac ccggtttggc
    14461 gctacgttta attttatgcc cgtcgccaaa atccagcagc gcatcgccgg tgatcatcac
    14521 gttatctttt tgcactttta acttacggac aaaatatgcg gcatcctgat gaaaaatatc
    14581 gcccgagttg agaaacaacg cgaacttgcc ttgcgccatt tcaatacctt tgttcatggc
    14641 gtcgtagata ccgttatctg gttcgctgac aaagtgtaag ttatagatac cattgagatt
    14701 ttccagatac tcacgggtgc cgtcgttgga accgccatca acaacaatcc attcgaagct
    14761 gatatcgtcc gcctgcgcca gatgcgccag cgaggcgtgt gttttgacta ttccttcgag
    14821 gttacgaaac gcgacagtga ttatgctaag caacatgttt tacctcgtaa tacccgtcat
    14881 acttcaagct gcatgtgcgt tggttttcct cgctcaccca cttcctgcaa ctcgaattat
    14941 ttagggtgta tatttaacgc ttttcgcaaa ataaacgggc agacgattaa aaacgcatat
    15001 tctgggctaa atatcgaacc ggtaaaaaac agcgataccg gagtaaaaag atataattgc
    15061 acgcgaaaat tgcggttatc accaaaagcg ttgatcatca tttttatcac ttttcccatg
    15121 taccacagcg ataaaaacac tgcgaaccag gaaaaataaa taatcagcag atacaaacca
    15181 ttgtctatgg tttttccgac atccgcaccg ttaaatattc cgaatgatgc gacatattca
    15241 taaagtgagc caaatctgac tacaccgtca atatgggtta aagaataacc gaccatcacc
    15301 agcggaccga caatacgata atacgatgac gatccttctg tacctaaatc gcccagacgg
    15361 gtggaaatat aagggaacgc gattaccaca ccaaccagga atacagccag agaaatcaat
    15421 gctaacggta actttttctt aatcgcctct ttattcagat attgaaatgc ccactccagc
    15481 aaataaaaaa ggataaaggt cataacccct gaaaatgaac cggataatat tatccctgcg
    15541 agaatcatag catctgtttt aggcgttttg ataccaaact gtttgatgct gagccaaatt
    15601 gagattaatg ccagagcgaa aaatgccggt tcgaaataaa gtgctgtcgt gcgcttgccg
    15661 ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa tcagatattt cgaaatcgac
    15721 tccataatac tgctaccgcc ggtgaggata atttgcgcca tctccaccgc cgccagcacc
    15781 accaccagcc cgaccaccag ataaaagaaa cgtaatatct tgcgatggtt gtgtggcgaa
    15841 atcgttttaa agcgaatact ccacaccatg ccaataatga tcacaatata gacaaacagc
    15901 atggttgaag tgacgtattt actggcatcc agcgactgac caaacaggta gttaaacgcc
    15961 gtaagtcccg cgccaatccc caaggcaatc atcaattttt taacgctgat gcgctctaaa
    16021 aacagcagta acaggacagg taaaaaagtg acgatggtga tggggaaact ttcgccaagc
    16081 tgggcgattt tgacgttaac cagcaggtag atcagcggca gcagcaggta gctacagatt
    16141 ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg cgcggctgcg
    16201 ctggctgaat tcagccagcg tggtaccaaa tatcgcctgt gcgatttccg gtttgcttaa
    16261 ctgcaccagt tgcagcacat cttcttcgct gacggtttta ccaccggatt tttgcagcac
    16321 ttcccgcgcc gcatcgctat gggtggcgat caccggcacg ccaatcgata acgcctcaca
    16381 caaaatcagc gggtagttat cgacgcgaga actgaacacc agcgcatcca tctgattgag
    16441 cacgctcatt aacttgcgct tgtcagtttc aaagccgtga ttgacaacgt taccagcggt
    16501 gaacggcgag aacttaccaa aggtatgcag ttcgattttg tcacccagcg ccatcatctc
    16561 gcgtaccagt tgctggttag ttttgccgtc gtaacgcagg tcatgcgcca ccaccgcgat
    16621 tttcggcttg ccctgggttt cgcgtaccgg aggcaagtcc gccagaatcg cttcggttgc
    16681 catatcaatg ccattattga taatccggca acgccctgga ccgtacagac tattgaaagc
    16741 atcagccaca tgctggctgg gggaaataaa ctgacagccc agtgccagca tctcgcggaa
    16801 taactggcgt ttacccgcca ccagctggtg cgcgcgatca atcttcaccg gtggatagtt
    16861 atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt cggtaaaggc
    16921 gcagcgtccg gtaacgctcc agtggtcgtg tagcgtccag accagagtga catccggttt
    16981 atggtttttc actttttcgc aaaagcgcac cacgctctta agattcaacc agtagctgtg
    17041 cagcacatga aaatgcagaa ccaccgggcc cggtgtgcga gtaatggtgc gatataactc
    17101 attgaaattg ccgaacagat cgcgattaag cagacgaaac aacgcaatgt tcgccatcgc
    17161 ggtcatccgt ggcgtatgtt tgatgacctg cggatagttc tgatggctga cgctctcttt
    17221 gccgcctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca gcgcgcgctg
    17281 gtggagatct aatgccacac ctgctgcccc gccttccgcc agtcgcacat taaattgcag
    17341 aatattcatt taattacctt cactcgcgct ttttctccca ccaccagcgc gttgtccggg
    17401 acagagtcga gcaccacgct gcccgcgccc acggtgacgt tgttaccaat cgtgatatca
    17461 ccaagaataa tgacgttggc accgagttcg acgccgttgc caatgtgtgg acatgccatg
    17521 ttatcggcgc cacgattgcc gatagtgacg ccgtgacgaa tggtgaaatc atcccccgcg
    17581 accacgtttt tattgatcac tacggcgtaa ccgtgatgga tagtaaagcg tcggcctatg
    17641 gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg atacagcacc
    17701 agcagcgggg ccgcccaaag attgttgagg acgttctttt tgcgccacac cgaacaaaaa
    17761 tgagcggcac gataggcaag aaccatgcag cacgggcgta aactccagct gttggcgcgc
    17821 agatcttcca gcatctttag cgcccccgaa taccatcagc cagacgtttg ccgttacgca
    17881 ccgacagcag cgtcagcaaa gtgcgccagg tcatgcgttt attgcggatc tggtagaggg
    17941 taaacagctg atattttttg ctggcgcggt cgaatttgtc tttgtgcttg cggtaaaagt
    18001 ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcgccg tgattgatat
    18061 gcaggatctg cgtcgcctct tccactttcc acggttcgcc gtactccacc accatccgca
    18121 ggaagatgtc gtaatcctgt gccgctttca gttcggtatc gaacaagcac tctttgaaac
    18181 gccacgccca ggtaaagacc tgattgccaa tgatattgcg tttgtaaaac aggcggcgtg
    18241 aatacggcga tttgggatac agcggcaagc ttgccggttg ggaataaact tcgccctggc
    18301 aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag ttgctgttta tgggcgagga
    18361 agacgctcag gcggttgggt gtccattcat catcgtcatc gatcccggtg atgtattccc
    18421 cttgcgccag cataatcgcc tggttacgta ccgcgcacgc cccgctgtta atgtcgttgt
    18481 gaatgtaagt gatacgcgga tcgttgaggg cggtgacgta ctgttgtagt tgttcccagg
    18541 aggtggagca atcatccacg atgatcatct cccagttgtt gtagtcctgg cgcaggaccg
    18601 attttatcgc ccgaatcgcc agttgttggc ggttccaggt cggcatatag attgaaatca
    18661 gcggattgtc tttcataggt tgttctcccg gagtatcgag atgttctgcc ggtttataag
    18721 ctggtttaca ggttctgttg ccggatgcgg cgtgaacgta ggtcggataa gatgcgtcag
    18781 catcgcatcc gacagcggat ctcgttattt cgcatccgac ttatattcgt attcgtaata
    18841 gccataatcc tgatacgcgc tggcgcggcg gaagatggag ttcagaatca cccctttcac
    18901 cggaataccg ttttgctcaa agcggctcag actggtttcc acttctttca atgtgttgac
    18961 cgcataacgc gccaccatca acgtggttcc gacatgacga ccaacaattg ccgcatcggt
    19021 cactgccaga atcggcggcg tatcaatcaa caccaggtcg tagtttttac tcgcccactt
    19081 caccagttcg gcaaagcgtt cgctcattaa cagttcagaa ggatttggcg gtacctgacc
    19141 gcgcgggatc aggtcaaatt tggcaataga ggtcggttta gcagcggtag taatatcgcc
    19201 ctgtccaatc agaatttccg acaggccatt aacgttatta gtgcccaaca gctcgtgggt
    19261 gtagcctttg cgcatatcgc agtcgatcaa caacacccgt ttattggtct ggctaatcac
    19321 cgccgccagg ttggcgcaga caaaggtttt accgattgac gggctaaccc cggtcatcat
    19381 caacacattg ttctgtgcct gcatcatcgc gaagtgcaga ctggtacgca gactacggat
    19441 ggcttcaatc gccagatcgg ttggattccc caccgccagt agctggctct gtttatagcg
    19501 tttaatccct ttgatggttt tgacgctatc gcgcgctttc tgccactccg acagcgggat
    19561 gctggcataa acgctgatac cgtgttcttc cagcacctgc gggctttcga taccgcgatt
    19621 aaacaacgag cgcagcagca cacccacgat agagagcatc aggccaagga taatcgcccc
    19681 aaggataatc agcactttct tcggtttcag tacacctggc tgggtgattg ccgggtcgac
    19741 aatgcgcaca tcgccgacgg tgctggcttc ggtgattttc agctcctgct ctttattcag
    19801 cagttgcata tagacctgct gaccagactc gacatcgcgg gtcagacgca caatttcctg
    19861 ctgggttttc ggcatcgccg ttacgcgacc attaagtttg gctttttcgt cttccagcgc
    19921 ctgccgtttc tccagcagcg tgcggtacgc cgggtgaact ttggtgtaca gcttggagat
    19981 ttccgcctct ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca tcgaatcgag
    20041 caccgctttc gcttccagcg gcagatcaac tgaatcttta tcctgacgga aggcattcag
    20101 tttgttttcg gcaacatcaa ggcggctacg tacttccggt aactgttgcg cgaggaaagc
    20161 gaggcttttc gacgcttccg ccgatttgcg ctcaatattt tgttcctgat agttacgggc
    20221 gatgctgtta agaatgtcgc ggatctgttc gcgatcttca ccggtgtaag tcaggctcag
    20281 aacacctgcg tctttgccgt tctccgttac cgtcaggctg ttttgcagtt ggttgatcat
    20341 ccccagcgtg gagtatttgg tgacggtaaa ctcactgccc gggctggcgt gaatggcttc
    20401 aaccatcagc gtgacgcctt cttttttcag catctggccc gcttgcccac gggcgctaaa
    20461 gccgccatcg ctgctcaggg tgtagttttt gttgtccagc acattaagcg taaacacctg
    20521 atccgccatc tccttcggtc ggttaaaggt ggtcactttc accgtctcgt tctggcgtcc
    20581 cgtcaggcgc tcccagcccg caccgaaaat cgggaacgtg tttttgctca ccgcaatatc
    20641 aaggtcgaga tcgtccaccg ttttaccaag caccagacgc gaacgaatca actggatctc
    20701 ggcgtccgat gcaggcggtt tgttggctaa tgccgatccg atatcctgca ctaacgaatt
    20761 gccgctgctt tgttcgattt gtaccagtgc gtcggcgctg tatatcggcg tagcgaagaa
    20821 ggtgtaaacc acggcacaga gggcgaatac ggcggtaatg ccaatcaccc accagcgtgc
    20881 ttcaatgatg gtgccaacca ggcgaccaat atcgatttca tcactgcccg ttaccggagc
    20941 ggcatgttgt tttacttttt ctgtcattct tatacctgct ctgcgttcaa tgcctgcgcc
    21001 cactggtggg cagaccgttc aagtaatgcg tacaccgctg caaacgtttc ccggcttttg
    21061 cgatatggat cggggatttc acattcgtta tcccagtgac caaacagcat cactttgccg
    21121 cgcatctcgg gtgccatctc gcataagcgt tcgatatggc gcttttccat ggtcagaatc
    21181 aggtcgtagt tgcgacacag gcggcggctg atttgccgtg cacagtgacc ttccagggac
    21241 agttgatgtt ctgcggcgac gctgatagcg gtaggatcag cgcccttacc gaccaacgcg
    21301 ccgagtccag cggactccac ttttagctcc gggtgataac gttgcagtaa gcgttccgcc
    21361 gtcggggaac ggcaaatatt gccgacacag acaactaaga tgttgttaaa catgacgatt
    21421 accagttatg aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta atagttggca
    21481 gcaactgatt gatcagacgg ttccagcggg ataccggggc ggtggtgaca tacaccacgt
    21541 cataaggttg caggcggaat tctgtcgcca ttaccagcga cgtcgcatcg gacatatcca
    21601 gctggtagat attggcaatc ttgccgttac gcccgctctc gcctttcagc ggacgaatga
    21661 caaagatgcc gctggcgttg gaggtggtca tgtcgatgcc ttcagcattg cccagggctt
    21721 cagtcagggt catgccgcta aagtccattt tcagggtgct ctgtttcttc acttcaccca
    21781 tcacaaacac tttcagatca tcattacgtg gcacgtaaag aatatcgccg gggtaaagca
    21841 gtcggttctg attgaggtcg ccgttttgca tcagcgcctg caaagaaatg cgctcttcac
    21901 gaccattgtg tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg
    21961 cgttgatggc gtcgagaatg gtcagtggca cgttggtaat ggcctgttga ccggatttat
    22021 tcacctgacc ggagatatag gctttttgcg agcggaaggc ggcgatatta acgtccacct
    22081 gcgggtcagc gatgtacgtc gctaagcgcc cggtaatatc actgcggatt tcagcgagcg
    22141 ttttccccac cacgtgaact ttgccgatat acgggtagaa catggtgccg tcaggctgta
    22201 cccagttgcc ggtgtcgctg gagctgcggt actgaccggc tggcgtggtg agttccgggt
    22261 gatcccagac ggtgacatta agaacgtccc ccggcccgac gcgatactgg taattcgcga
    22321 tctcgctttc cagcgtcata ttggggcgcg ctacattcgg gcgtgggcgt aattgatcaa
    22381 tcaggcgcgg ggtcagcgga taaacattca ccattttgtc gagatcgaaa tcagcgtcct
    22441 gctgtttgat gacgtctttc cccagcgtcg acatattgct gcccggaagt actgtgcaac
    22501 cgcttatcaa ggttactgac accaataatg gcatcaattt cattttggat ttcatcattg
    22561 tttatttatc actttggcag agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg
    22621 cacatttacc ttgcagttaa ttgaataaaa atttaactgg catcagtcct aaaaaaaatt
    22681 gatttcatcc gcaggctatt gacagaataa ttcagactgg tctttcaggc atccagacac
    22741 gctaccgccc ctggcttttt agctaccaac acactgattt agcttaattt ttcacaccct
    22801 ctctgcatac agtcgttgat gagaaagggt tattgcggaa attaacttcc gaatataagg
    22861 tgacattatg gtaattgaat attggctctc caataatgca ggaggaagtg ttacagctaa
    22921 cggaatagca ggcaaggtaa caattcggca attggctatt tttaagaatt atattaagtg
    22981 tcgttcaata tggtttttag gagtttcttt agattgacaa tatttaatat agtgtctcca
    23041 catgcgatat ttcttaaata atgttttatt attagcacct ttaattctgg gataatgtga
    23101 ggttataacc ctcaaataat atgagataaa tagtgctgca acattgcatt tttgccccga
    23161 tttatccatg atcgaattgt gacatttgtc atacaacgaa taggttttgt acttactatg
    23221 gaatggattg ccgatccgtc tatctgggct gggttaatca cgctgattgt gatcgaactg
    23281 gtcctcggca ttgataacct ggtctttatt gccatcctcg ccgaaaaact gccgccgaag
    23341 cagcgcgacc gcgcacgggt taccgggctg ctgctggcga tgttaatgcg cctgttactg
    23401 ctggcgtcaa tctcctggct ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt
    23461 acctttagcg cccgcgattt aatcatgctg tttggtggtt tcttcctgct gttcaaagcc
    23521 acgatggagc tgaacgaacg gctggaaggg aaagacagca ataaccccac gcaacgcaaa
    23581 ggtgcgaagt tctggggggt ggtgacacaa attgtggtac tggacgccat cttctcactc
    23641 gactcggtga ttaccgccgt cgggatggtc gaccatttac tggtcatgat ggccgccgtg
    23701 gttatcgcta tcagcctgat gttgatggcc agcaagccgt taacgcaatt tgttaacagt
    23761 cacccgacca tcgttattct ctgcctaagc ttcctgttga tgattggctt tagcctggtg
    23821 gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt acgctgctat tggtttctcg
    23881 gtgatgatcg aggcgctcaa tcagttggct atctttaacc ggcgacgttt tctttccgct
    23941 aatcagacgc tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag tgggcaaaaa
    24001 gaagatgcgg aactggacgc cgaaaccgcg tctatgctgg tggatcatgg taaccagcaa
    24061 atctttaatc cgcaggaacg gcggatgatt gagcgggtac ttaatcttaa ccagcgtacc
    24121 gtcagcagca ttatgacgtc gcgccacgat attgagcata tcgatctcaa cgcgccggaa
    24181 gacgagatcc gccagttact ggagcgaaat cagcatacgc ggcttgtcgt tactgatggc
    24241 gatgacgcag aagatttgct cggtgttgtt cacgttatcg acctgctaca acagtcactg
    24301 cgcggcgaac cgctcaacct gcgggtgttg attcgccagc cgctggtgtt cccggaaacc
    24361 ttgccgttgt tacctgccct ggagcagttc cgtaatgccc gcacacactt tgcttttgtg
    24421 gtggatgagt ttggctcggt ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt
    24481 gccggtaact taccgaacga agtggaagag atcgacgccc gccatgatat tcagaagaat
    24541 gcggacggtt cctggacggc gaatggtcat atgccgctgg aagatctggt gcaatatgtg
    24601 ccgttgccgc tggatgaaaa acgtgaatat cacaccattg ccgggctatt gatggaatat
    24661 ttgcaacgta ttccaaaacc tggcgaagaa gttcaggtgg gggattattt gcttaaaacg
    24721 ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta ttccgctgcg taaggatggc
    24781 gagatggagt acgaggtgtg atggcatcgg ccattttacc caatgctgcg taacgcctta
    24841 taacttcagc aatgaagcgt tgtctgtggt acatggacat cacagcgggg gtgtgggtcc
    24901 ggctgtaaat cgccgaatcg tttcctgtag gccggggcag cccacatgga tgtgggctga
    24961 gggcgcgttt tacagggatg ttacctcgcg cccgtacccg atagccgaaa ggaataagat
    25021 gagggaaccg cgccagcggc gatttaccgc cgggagcccg ggttgccagg gtggtggcga
    25081 ttgagccacc ctggcacgtt caccggcttg agcgttccat agtagcgagg aacatcaagt
    25141 gaacggaaca gccttcgatc ttacatattc agaatataat ggtgccgcca cgaacgcctg
    25201 atgcgacgct ttacgcgtct tatcaggcct acaggacagg tcacctggta acctacatct
    25261 tctccagcaa cttcttcaca tctttgccat tgcgggaatc tttattccgc tcggcccagt
    25321 cgttaagacg gcgttttgct tcatcctgta gatgtttacg cagtaactga tccacttgca
    25381 ggctgtaatt aagttgctgc cagttgccat aaacccgcag cggtactggc gtttctttga
    25441 ggaaatcaat cagtttgctt tccccgttcc agccacccac gacccgaata tcaaactggg
    25501 tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc tgtcagcgcc agcacaggcg
    25561 attgaccttg catgtcgttt aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa
    25621 agcggtcaag acgcgtcacg ttatcgaagt tttcagcggc cttcacatca ccaccattac
    25681 gttctaccgc ttgctgaatc atctgctgga agttcatccc ttccatgcgt gtatcagtca
    25741 tttcgacatg tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct atgtcagcac
    25801 cggagaaatc accagccagt gacatctttc cggtcaacga aatcggatag ttaaacgcct
    25861 tcagaatggt gccaatctca acgttttcca gccgtggctg gaagtttatc cgcggattta
    25921 ttgatgtcgc gtccaacgtg cccggcagtg aaacctgtcc accgttaagt ttgccctgca
    25981 gttgagtaat ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat
    26041 tcattccgcg ccagcgcacg ttactggcct gcaacaaaat atcagccgta aagccttgca
    26101 gtccctgata ggccggttca tcaatacgcg aagaaatgac cgggcgcggc aacgttgatt
    26161 ggctctgccc ttgctgcgcg gcaccgttct caccgttcgc tgtttcatta agggggatga
    26221 ggttgtcaag attcagttgc gggaattgca gtctcagctg ccattccggt ttctctgtca
    26281 acgtgacctg tgcttgcccg ctcagcgtac tgtcattggc ggtcaaactg atttggttaa
    26341 atgaaaggcg tttatgtgac tcctgccact gggcctggaa actcccctgc ccctgaatac
    26401 cttgttttgg taaatcggcc ccctgcaact gccagttaat ttgttcaata gccgccgtta
    26461 aatcatgcgg ataatcagaa gcatctactg taccgttaag ggataatgtg agatcgcgct
    26521 gatcgcgatt aacccgcccg gaaaactcaa atgagccacg atgttgggga tcttgttcca
    26581 tttgcaggcg gatattgcgg attgtcacct gctcgtcatc ttcatgctgg aacaccagaa
    26641 cgctgtccgc caccttaaga ctggatatat caaacgacca tccgcgatcg tctgacagat
    26701 ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct
    26761 gcggcgtcag ttggatcact gcccctttta gcatcacctg cttaacgctc agttgatgac
    26821 tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc ggcgcgaacc agtggctggc
    26881 ttgcgccctg ggcggtgaga gacattcgcc cggagaggat actaagctgc ggccagacgt
    26941 gccaacgcag tggcccgtcg agctgcaatt gataaccgct acgtgcagca acttgcttga
    27001 ccatatagtc gcggaaatca ttcggattca ccagcaacac taacgcagat aacccggcca
    27061 ccagcacgac caggagtatc atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg
    27121 gcctgaatta gtctttatcg attcggctgg ctaccgcgcc ctgctggttg cgatatttcg
    27181 catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga gagcggctca aagctcagcg
    27241 caccaattaa catgcccgga cgcagcgcca gcggcagctt accggagttg taaaactcca
    27301 gcacaatgca gccagaccag cccggatcga tacggtgcgc ggtgacgtgc accatcagcc
    27361 ccagacgcgc cagtgaagaa cgcccgtcca gccagcccac cagatcggct ggcagcgtca
    27421 ccgactccag cgtcaccgcc agcgccagct ctcctgggtg aagataaaac gcctcgccct
    27481 cgtcgagcac gatttcatcg ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg
    27541 gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat ttattgccca
    27601 ggcgcacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg ttgatcgaca
    27661 aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg gcattttctc
    27721 ctttcgagca tcactccctt aacgccaatt acgtcaaggg cataccaggt tatcactgaa
    27781 cggtacacaa tttatcaagc ttattcaaag aactgactta ttttcgcttt caatatatcg
    27841 atcgcgatgc ggtttttccc gccgcgcggc acgataatgt ccgcatattg tttagaaggc
    27901 tcaatgaatt gcaggaacat cgggcgcacg gttttttgat attgcgccat cactgaatcc
    27961 attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc gcatcagaca gatatccagc
    28021 ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgta aacgcgcatc cgtcagcaac
    28081 aaaatgcctt cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc tttcatacgc
    28141 gtatgttcaa catagctgta aaccggcagg tcaattgccg agccgcgttt caacgcttgt
    28201 aaatgctcaa gcagcaggct gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg
    28261 cgttcttcca tcgacagatg gctttgatct ttgtaatagc agtcttcggg aattacgccg
    28321 atgtgttcat caccgacttg ctcacgtaat tcacgataaa gggtactggc aataagactc
    28381 ttgccggaag ccgatgcgcc agcgataccg ataatgacgc actgatgaga ctgatcagtc
    28441 atatatttag cgacctgatt aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc
    28501 ggcaattata gggatttcat ccgcctgata ccagtcgaat agcgttgccg cgcgctcaga
    28561 gttaattgtt gacgaagaat tcccggtggc aaattacgtt gatcagtttt atacaaggta
    28621 aaaaatgtta tacgcagttg cgcaaattat ccgcctttac gtcactttat gagcaattcg
    28681 catataaaat gtaaaacttt tgtactagca tgaacacaga aacgaatact gacgacctgg
    28741 ccttgcggat aaagcggtaa tgagcaaaca atcacagcat gtattaattg ccctgcccca
    28801 cccgctgctt cacctggtca gtttaggttt agtctcgttt atcttcaccc ttttctcact
    28861 tgagctttcg cagtttggca cccaactcgc cccactgtgg ttcccgacgt ccatcatgat
    28921 ggtggcgttt tatcgccatg ccgggcgcat gtggccggga attgcgctga gctgctcgct
    28981 gggaaatatc gccgcatcca tcctgctttt ttccaccagc tcgctgaaca tgacctggac
    29041 gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat tgctgccgtg
    29101 gtataacccc ttgcaaaatc tggctgactg gctgcgtctg gcactcggca gcgccattat
    29161 tccgcctctg ttggggggtg ttctggttgt cctgctgacg cccggagacg atcctctcag
    29221 ggcatttttg atatgggtac tgtcagaatc catcggcgct ctggcactgg tgccgctggg
    29281 attgttattt aaaccacact atctgctgcg ccatcgcaac ccaaggttgc tttttgagtc
    29341 gctgctcacg ttagctatca cgctgacgtt aagctggctt tcgatgctgt atctgccgtg
    29401 gccttttact ttcattattg tgctgttgat gtggagcgcc gtgcgcctgc cacgaatgga
    29461 agcctttttg atcttcctta ccacagtgat gatggtatcg ctgatgatgg ccgcggatcc
    29521 ctctctgctt gctacgccgc gtacgtacct gatgagtcat atgccgtggc taccgttttt
    29581 gctgatcctg ctgcccgcca acatcatgac catggtgatg tatgcctttc gtgcggaacg
    29641 caaacacatt tccgaaagcg aaacccgctt tcggaacgcg atggaatatt ccgccatcgg
    29701 catggcatta gtgggcaccg agggacaatg gctgcaatcc aacaaagcgc tctgccagtt
    29761 tctcggttac agtcaggaag aactgcgcgg actcaccttt cagcaactga cctggccgga
    29821 ggatctcaat aaagatctgc aacaggttga aaagctgatc agcggtgaaa taaacaccta
    29881 ttcaatggaa aaacgctact acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt
    29941 ctcactggtg cgccacacgg atggcacgcc gctctatttt atcgctcaga ttgaagacat
    30001 taacgagcta aaacgcaccg aacaggtgaa ccagcaactg atggagcgca tcacgctggc
    30061 taacgaagcg ggcgggattg gcatctggga gtgggagcta aagccgaata tttttagctg
    30121 ggataagcgg atgttcgagc tgtatgaaat tcctccgcat atcaaaccga actggcaggt
    30181 gtggtacgag tgcgtgctgc cggaagatcg ccagcacgcc gaaaaagtga ttcgcgattc
    30241 attgcaatca cgctctccct ttaaactgga atttcgcatt accgtgaaag acggcattcg
    30301 ccatatccgc gccctcgcta accgggtact gaataaagaa ggcgaagtcg aacgcctgct
    30361 cggcattaat atggatatga ccgaggttaa acagcttaac gaggcattgt ttcaggaaaa
    30421 agagcgcctg cacattacgc tggattccat cggtgaagcc gtggtctgta ttgatatggc
    30481 gatgaaaatt acctttatga atccggtggc ggagaagatg agcggctgga cgcaggaaga
    30541 agcgttaggt gttccgctcc tgacggtgtt gcatattact tttggcgaca acggaccatt
    30601 aatggagaac atttacagtg ccgacacctc acgttccgcg attgaacaag atgtggtgtt
    30661 gcactgccga agcggcggca gctacgacgt gcattacagt attacgccgt taagtactct
    30721 ggacggcagc aatattggtt cggttctggt gattcaggac gtcaccgaat cgcgcaaaat
    30781 gctgcgccag ctgagctaca gcgcctccca tgatgcactg acgcatctcg ccaatcgagc
    30841 cagttttgag aagcaactac gcatcctgct gcaaacggta aacagtacac atcagcgaca
    30901 tgcactggtg tttatcgatc ttgatcgctt taaagcggtg aatgacagcg ccgggcacgc
    30961 cgctggcgac gctttactgc gcgaactggc gtcattgatg ctgagtatgc tgcgctccag
    31021 cgacgtgctg gcgcgactcg gtggcgatga atttggtctg ctgctgccag attgtaatgt
    31081 cgaaagtgcg cgttttatcg ctacacgcat tatcagcgcc gtgaatgact atcactttat
    31141 ctgggaagga cgtgtacatc gggtaggtgc cagtgcaggg attaccttga ttgatgacaa
    31201 caatcatcag gcggctgaag tgatgtcgca ggctgatatc gcctgttatg cctccaaaaa
    31261 tggtggccgg ggccgggtga cgatttacga accgcagcaa gttgccgcac atagcgaacg
    31321 ggctgtgatg tcgcttgatg aacagtggcg gatgattaaa gagaatcagt tgatgatgat
    31381 cgcccacggt gtcgcttcgc cgcggatccc gcaagcgcgt aatttgtggc tgatttcact
    31441 taagctctgg agttgcgaag gcgagattat tgatgaacaa acatttcgtc gtagcttcag
    31501 cgatccggca cttagccatg ctcttgaccg acgggtattc cacgattttt tccagcaggc
    31561 cgcaaaagcg attgccagta aaggcttaag catcgccctc cccctttccg ttgccggttt
    31621 gagtagcgcc acgctggtga atgaactaat tgagctgctg gaaaatagcc ctctatcacc
    31681 acggttatta catctgatta ttccggcaga cgcgatttta gatcacgcag aaagcgtgca
    31741 aaaactgcgg ctggcgggat gtcggatcgt attcagtcag gtgggccgcg atctgcaaat
    31801 cttcaactcg ctgaaagcga atatggcaga ttacctgcta cttgatggtg agttatgcgc
    31861 caacgtgcag ggaaatttga tggatgagat gctgattacg atcattcagg ggcacgctca
    31921 gcggctcggg atgaagacca tcgccgggcc agtcgtttta cccttagtga tggatacgct
    31981 ttctggcatc ggcgtcgatc tgatttatgg cgatgtgatt gccgatgccc aaccgctgga
    32041 tttgctggtg aatagcagtt atttcgcgat taactaaggg agcgtttgcc cattgcctga
    32101 tgcgacgcta acgcgtctta tcaggcctac aaatcgctca ttccccaggc cggataagct
    32161 cgcaccgcat ccggcaacca acgtcatgct tcgtctggtt gccagccttc cgtataccag
    32221 atatgcaaca acgcataaga acgccagggc ttccagcgtt cggcataacg gcggatttgt
    32281 gccggtgtca ttcccggaaa tcgctgttta atcagataat catccggcag aaaaacatct
    32341 ttcgcctgcc agccacgcaa agcaaaataa tttgccgtcc agcgcccgat acccggaaag
    32401 gtttgcagcg ttttcatcgc ttgttctacg tcgcccggta ttgtcattgg taaggtgccc
    32461 tccagcgccg catttgccag atgaatcagc gcctctgccc gtttcaacgg catacctaac
    32521 gcttttaatg cctgcgggtc ggctactgcc agccgctgag gcgtggggaa gcagacataa
    32581 tccggaaaat catccagccg ttcgccataa agctgtgcca ctctggaggt caattttgcc
    32641 gccatcgcca cgctcaccag ttggcctaaa atcgcccgca cgccctgctc aaaagcgtca
    32701 acacaaccag gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta
    32761 acaatctgcg ggttacattg cagatcaaac aggcggctca ttttcgccag acactcagcg
    32821 gcaacaggtt ctaaacctac acttaaattt atgtgcagag tatggcgggc tatatccgga
    32881 atagcagtca ccacgccgcg atattcgcct accgccagac tacgggcata ataactgtcc
    32941 gcgaccgttt ccacgccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag
    33001 tcatacggcg gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc
    33061 ataaacgtta ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acggcaacat
    33121 ttcgctaaag tcacgcccct tcttcaccgg catggggatt atttcgtgtt tattggtttt
    33181 gattacggta cagcaaactg ttcagtggcg gtcatgcgcg acggtaaacc gcatttgcta
    33241 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg
    33301 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
    33361 ctgttgcgtc gggcgattcg ttataaccgc gaagaagata tcgatgtcac ggcgaaaagc
    33421 gtgcagttcg gtctttcttc actggcacaa tacattgatg atccggaaga agtgtggttt
    33481 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
    33541 tttgaggatc tggtctgcgc aatgatgttg cacattcgcc aacaggcgca ggcgcagctg
    33601 ccggaggcaa ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggt
    33661 gatgaagcaa acacccaggc gcaagggatt ctggaacgcg cggcgaagcg tgccgggttt
    33721 aaggatgtgg tattccagta cgagccggtc gcggctgggc tggattacga agccaccttg
    33781 caggaagaaa aacgagtgct ggtagtggat atcggcggtg gtacgactga ctgttcattg
    33841 ctgctgatgg ggccgcaatg gcgttcgcgt ctcgatcgtg aagccagcct cctgggtcat
    33901 agtggttgcc gtattggtgg taacgatctg gatatcgcac tggcgtttaa gaacctgatg
    33961 ccgttactgg gcatgggggg cgaaaccgaa aaaggcatcg ccctgccgat tctgccgtgg
    34021 tggaatgcgg ttgccatcaa cgacgtacct gcgcaaagtg atttctacag tagtgccaac
    34081 ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta
    34141 cagaaagtct ggcgtcagcg tttaagctat cgcctggtac gtagcgcaga agagagcaaa
    34201 attgcgcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag cgatgaactg
    34261 gctacgctga ttagccagca agggctggaa agcgccctca accagccact ggcgcggatt
    34321 ctggaacagg tgcaactggc gctggataac gcccaggaaa aaccggatgt tatctatctg
    34381 accggcggta gcgcccgttc accgctgatt aaaaaagcgc tgacagaaca gttgccgggc
    34441 attccgattg caggtggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg
    34501 gaagtggtgt ttcgttaatt gaaaactggc tcagcaacgg cttaaatcag cctgcgcctg
    34561 agccagtttt tctgccgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc
    34621 ttgaaggcgg aatcgctgca actctgtcgc accgacagtc agcgccgatt ccagccgatg
    34681 tcgtttggcg gccatttttg ccattaccgc ttgtctgtct gcaggcgtta ccgcttcgtt
    34741 cggcctgaaa acaaaacggc gttcgcaact cgctttccag tcgataaccg cctgggtcag
    34801 atgatcacca aaacccctaa cttgcttaac gctccgtcgg gtaacgtccg ctgctgtttc
    34861 aataccaaag gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac
    34921 atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcgg tgtcgtgaag
    34981 tgctgccaga tcgcgctttt cttcttccgg taacccaaga atttcgtcct tcattttttc
    35041 gagcatcgcc cgtttggcga taaaaccttc cagcccgccc aactgttgga tctggctgac
    35101 taaatggtca tagtccattt ttgcgcgatt aaaagcctct cttcgtcgct ggacttctac
    35161 tttgtacgct ttgcttgtca ggctgccaat aatccagata gccgccagca cgggaaccaa
    35221 aatgatataa cgcggttctg tctgaaggcc gcaaagcagc agcgataacc ctgacagtgc
    35281 gatctcaatc agaatgatgt attcgcgcct taacaggcct gaaggaagcg gcctgccagc
    35341 agcctggaaa tgatcgggta atggcaattg caatgctggc ggtgctactg acgccatcac
    35401 catcgcccag actttcgcca gcacaaaatt cccgccggtg gtaatgacct cttcgccgag
    35461 atcaataaaa tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg
    35521 accgggataa acatgcattg ccgaaacggt acatttcttt aactgttggc gtagtaaatc
    35581 aagtgccgct acccacgctt ttgccgtcgg acgcccggtt gccacgccac tttccgtgaa
    35641 tgcctgctga aacatggctt caacatcgcc cggtaacatc gacagcggaa tcgatcgtgg
    35701 cggcggtttt aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggcaat
    35761 atccgtctcc agcgcattac ccgcatcaga gataagcggt acaccggaat aaggatgccg
    35821 cccaccaaac aagacgtgaa atatcagcaa cgcaaggcca aaattatcgt gattttctgt
    35881 gcgttcaaag ccgacaaatg atggcatggt ttgcagctct ggcggcgtaa aatgcgacac
    35941 gccgacttcg cataaatgca gtgtaccatt ggcattaatc tgaaaggagt cgctatcaat
    36001 catcaccact ttgctgtcgc gacctaccat aaagctgttc tggtttacgt cacccacgac
    36061 gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag
    36121 aaaatcccac gcacaatgag ggtaactctg gcgacgatgt gccgggctat agatcatatg
    36181 aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc cacctcgtcc
    36241 accgtgaagc gttgcctgcg gccaggcgac ataattcaac aactgcgcgt cagctgtcgc
    36301 agccataaag gcaagtttgt cctgttttaa ggcgggaggc gacgtgtgat aaatcttggc
    36361 gacgctctcg acaaactcct cgatatcata aaccgcacct tcgccgcctt tgcccagttc
    36421 acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac
    36481 agcgccagtg ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa
    36541 aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca
    36601 ttgaaaaacg gggtaaagaa aggcatatgc ggagaattat ccagcatatt taacgccagt
    36661 cgctgaatac catccgtaaa tgctgcaact ttgtgcgcac gctcagtgct ggtgaaagta
    36721 tccagtctgg aaacggcatc ttcatcagta atgaagtgcg tcatattggc atattccccc
    36781 accatcggcg tgagcggcag ttgcaaaccg tgacccaaat ctaccaccac gccgccatcg
    36841 ccaatctgca taatcaaggt gccattagct gatgaaatca atcccaaaaa cgtgcaggca
    36901 aaatcgcgaa ccgctaattc tttggcttcc gcctcggcaa aaagtcgctg acgaatggtc
    36961 agcacaatgt ccgttgcgag gacatcatta agccccaatt ccccgccctg cactttttgc
    37021 gacatataag ccatcgcttc attgacggcg agcatcgcac cttcgcctcc ctgcgaggcg
    37081 ctacctgcgc catcagcgac aaataccgac aataatggct gctggtcatt tagccaggca
    37141 acctgtatct ggcaggcatc ctggcaaggc aagtccgcac tgatgtgcga cgttccaacg
    37201 gctgatgcat agaccagtcg ccagctcaca ctgacgtcca gccttttggc gcttccagca
    37261 caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa
    37321 acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca
    37381 gcgtcttcat atccgccccc tgaacgccga tggtaaagaa ggcaaacttc ttatcttctt
    37441 ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggtgca ccatcggtga
    37501 tcaggaaaat ccacggacga taataggaaa taccattggc acgatattca cgtttccgct
    37561 cctcaaccat atccagggct ttggtaatgg cagagcccat tggtgtatcg ccctgggcaa
    37621 acaggatggg cgggaaaaaa ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc
    37681 cgaacgtcac aatgccgagt tccactcttt ttaaggccag cgaatcggca agcagctcat
    37741 cgcgaaaggt aaccaatccg gcgttaagtt cattaatggg tctaccgttc atagaaccag
    37801 aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg
    37861 tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat tgaggtccgg
    37921 tatcataccg actgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc
    37981 ggcggcgcag aagattttgc tattcgcctc gcaagaacgt gggcttacag attgacaaca
    38041 agggttgagt gacataattt caggagtgag ggtagagcgg ggtttccccc gccctggtag
    38101 tcttagtatg ctgggtagct aatgactaag agcaccacga tgatgagtag cttcatcatg
    38161 accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct
    38221 gaaatcttcg gcttacctcc tttcgccctg tatgcagtct atcgctgact tgcggataaa
    38281 atagtttcaa gtgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc
    38341 agactgcaaa gtctgcggat tgacaattgg accgtgaagg catactttca ggaatgaggg
    38401 ttgagcgggg tttcccccgc cctggtagtc ttagtaagcc gggaagctta tgactaagag
    38461 caccacgatg atgatccact tcatcatgac cctttcctta tttatggccc cttcctcggg
    38521 aggggctttc ccgtttcagc gtcccgctga aatcgtcggc ttatctcctt tcgccatgca
    38581 agcagtctat cgctaacgcg tagataaaat agtttcatgt gttattactg gatgcgtgct
    38641 cgcaaaagtg cccgtcattc agacgattcc cgacagtgtt tcataattcc tccatttttc
    38701 tcccttattg gctggctaca ctagtatcat tccgcgaaac gtttcaggaa gagaaactct
    38761 taacgatgaa aggcagttat aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg
    38821 ccatcgccgc attctggttc tggcaaggcc gcaatgactc ccagagtgca gcccctgggg
    38881 cgacgaaaca agcgcagcaa tcgccagcgg gtggtcgccg tggtatgcgt gccggcccat
    38941 tagctcctgt tcaggcggcg accgcagtag aacaggctgt tccacgttac ctcaccgggc
    39001 ttggcaccat taccgccgct aatactgtta cggtgcgcag ccgcgtggac ggtcaactga
    39061 tggcgttaca tttccaggaa ggccagcagg tcaaagccgg cgatttactg gcagaaattg
    39121 acccaagcca gttcaaagtt gcattagcac aagcccaggg ccaactggca aaagataaag
    39181 ccacgctcgc caacgcccgc cgtgacctgg cgcgttatca acaactggca aaaaccaatc
    39241 tcgtatctcg tcaggaactg gatgcccaac aagcgctggt cagtgaaacc gaaggcacca
    39301 ttaaggctga tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgtatca
    39361 ccgcaccagt tgatggtcgc gttggtctca agcaggttga tgttggtaac caaatctcca
    39421 gtggcgacac caccgggatt gtggtgatca cccaaacaca tcctatcgat ctggtcttta
    39481 ctctgccgga aagcgatatc gccaccgttg tgcaggcgca aaaagccgga aaaccgctgg
    39541 tggtagaagc ctgggatcgc accaactcga agaaattaag tgaaggcaca ctgttaagcc
    39601 ttgataacca aatcgatgcc actaccggta cgattaaagt gaaagcacgc tttaataatc
    39661 aggatgatgc gctgtttccc aatcagtttg ttaacgcacg catgttagtc gacaccgaac
    39721 aaaacgccgt ggtgatccca accgccgccc tgcaaatggg caacgaaggc cattttgtct
    39781 gggtgctgaa tagcgaaaac aaggtcagca aacatctggt gacaccaggc attcaggaca
    39841 gtcagaaagt ggtgatccgc gcaggtattt ctgcgggcga ccgcgtagtg acggacggaa
    39901 ttgatcgcct gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccactactc
    39961 cggaagaaaa agccgccagc cgcgaagaca cgaaaaaagg agcacgctcc tgatgcaggt
    40021 gttacccccg agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac
    40081 cacgctgctg atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt
    40141 ttcggcgctg ccggaagtgg actatccaac cattcaggtg gtcacgcttt atccaggtgc
    40201 aagcccggat gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat
    40261 gtctggcctg aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttaca
    40321 gttccagcta acattgccgc tggatgtcgc cgagcaggaa gtgcaggccg cgattaacgc
    40381 tgcgaccaac ctgctaccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc
    40441 ggcagatcca ccgatcatga cgctcgctgt cacctcaacc gccatgccga tgacgcaggt
    40501 ggaagatatg gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtagggct
    40561 ggtgacactt tccggcgggc agcgtccggc ggtacgcgta aaactcaacg ctcaggcaat
    40621 tgccgctctc ggcctgacca gcgaaagtgt gcgcaccgcc attactggcg cgaacgttaa
    40681 ctcagcgaaa gggagccttg acggcccttc tcgcgcggtc acgctgtcag cgaatgacca
    40741 gatgcaatcc gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcaccaat
    40801 tcgtcttggc gatgtcgcaa ccgtagagca aggagcagaa aacagctggc tcggcgcgtg
    40861 ggcgaacaaa gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat
    40921 ctccaccgcc gacagcatcc ggcagatgct accacagctc accgagagtc tgccgaaatc
    40981 ggtgaaggtg acggtacttt ctgaccgtac caccaatatc cgcgcatcgg tcgacgatac
    41041 ccagtttgag ctgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg
    41101 caatattccg gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa ttggcacctt
    41161 tgcggtcatg gtgtttctcg atttttcaat caataacctg acgttgatgg cgttaactat
    41221 cgccaccgga ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat
    41281 cgaaaaaggc gaaaaaccgc tggcagccgc gctcaaaggc gcgggtgaaa tcggctttac
    41341 cattatctcg ctgaccttct cgctgattgc ggtattgatc cccctgctgt ttatgggcga
    41401 tatcgtcggg cgactgttcc gcgaatttgc tattaccctg gcagtcgcga ttttgatctc
    41461 agcagtggtg tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc
    41521 gctgcgtaaa cagaaccgct tctcccgtgc ctcggagaaa atgttcgaca ggataatcgc
    41581 cgcctatggt cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtagc
    41641 gctcagcacg ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc
    41701 ggtacaggac aacggcataa ttcagggcac tttgcaggca ccgcaatcca gctcctttgc
    41761 caatatggcc cagcggcaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca
    41821 aagtttgacc tcatttgttg gcgttgatgg cactaacccg tcgctgaaca gtgcgcgttt
    41881 acagatcaat ctcaaaccac tggatgaacg agatgaccgg gtgcaaaaag tcatcgcccg
    41941 tctgcaaacg gcggtggata aagtgccggg cgtagatctc ttcctgcaac caacgcagga
    42001 cctgactatt gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc
    42061 actggatgcg ctcagtacct gggtgccaca gttgatggaa aaactccagc agctgccaca
    42121 gctttctgat gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgtcga
    42181 cagggacagc gccagccgtc tggggatcag catggcggat gtcgataacg ccctgtacaa
    42241 cgcgtttggt cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt
    42301 gctggaacac aacaccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac
    42361 cagcagcgac ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc
    42421 gccgctttcc atcaaccatc tggatcagtt cccggtaacg actatctcct ttaacgtgcc
    42481 ggataactat tcgctgggcg atgcggtgca ggcgattatg gacacagaga agacgctgaa
    42541 tctgccggtg gatatcacca cgcagttcca gggcagcacc ctcgccttcc agtcggcgct
    42601 gggcagcact gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat
    42661 tctgtacgag agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt
    42721 cggcgcgctg ctggcgttaa tgattgccgg tagcgaactg gatgtgattg ccattatcgg
    42781 cattattttg ctgatcggta tcgtgaagaa gaacgccatc atgatgatcg acttcgcgct
    42841 ggctgctgag cgcgagcaag gcatgtcgcc gcgcgaagct atttaccagg cctgtctgtt
    42901 gcgttttcgt ccgatcctga tgaccactct ggcggctctg cttggtgcgc tgccgctgat
    42961 gttgagtacc ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg
    43021 tctgattgtc agccaggtgc tgacgctgtt taccacgccg gtgatttatc tgttgttcga
    43081 ccgtctggca ttgtggacca aaagccgttt tgcccgtcat gaagaggagg cgtaagtgaa
    43141 gttttttgcc ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac
    43201 cctgtgcggc atactgggtt tccgtatgct gccagtcgcc ccgctgccgc aggtcgattt
    43261 tccggtgatc atggtcagcg cctcgctgcc cggtgcgtca ccagaaacaa tggcgtcttc
    43321 cgttgccacg ccgctggagc gctcacttgg gcgcattgcc ggagtcagcg aaatgacctc
    43381 cagcagttcg ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa
    43441 cggcgcagcg cgcgatgtgc aggcggcgat caacgccgcg caaagtctgc tacccagcgg
    43501 gatgcccagc cgtccgacct atcgcaaagc gaacccatcg gatgcgccaa ttatgatcct
    43561 cacgctgaca tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacacagct
    43621 ggctccgacg atttcgcaaa tcgacggtgt cggtgatgtc gatgttggcg gcagctcgct
    43681 gcccgccgta cgcgtcgggc tgaatccgca ggcgctgttt aatcagggcg tctcgctgga
    43741 cgacgtacgc accgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga
    43801 tggtactcac cgctggcaga tccagactaa cgatgaactg aaaaccgccg ctgaatatca
    43861 gccgctgatt attcattaca acaacggcgg agcggttcgt ctgggcgatg tggcgacggt
    43921 gaccgactca gtacaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt
    43981 gctgatgatc cgcaagctgc cggaagccaa tattatccag acggtagaca gcatccgcgc
    44041 ccgtctgccg gaattacaat ccaccatccc ggcagcaatt gatctgcaaa ttgcccaaga
    44101 tcgctcccct actatccgcg cgtcgctgga agaggttgag cagacgctgg taatctcagt
    44161 tgcactggtg attctggtgg tgtttttgtt cctgcgttcg ggtcgcgcca ccattattcc
    44221 cgccgttgcg gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgtggttt
    44281 cagtctcaat aatctttcgt taatggcgct cactatcgcc actggctttg tggtggatga
    44341 cgccatcgtg gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccgctaca
    44401 ggccgcgcta caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact
    44461 ggtggcggtg ttcctgccgc tgctgttgat gggcggattg ccgggtcgcc tgttacgcga
    44521 gtttgccgtg acgctttctg tcgccattgg tatttcgctg ctggtttccc tgacgttaac
    44581 gccgatgatg tgcggctgga tgctaaaagc cagcaagccg cgcgagcaaa agcgactgcg
    44641 tggctttggt cgcatgttgg tagccctgca acaaggctat ggcaagtcgc tgaaatgggt
    44701 gctcaatcat acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctg
    44761 gctgtatatc tcgatcccga aaaccttctt cccggagcag gacaccggcg tactgatggg
    44821 tgggatacag gctgaccaga gtatttcgtt tcaggcgatg cgcggtaagt tgcaggactt
    44881 catgaaaatt atccgtgacg atccggcggt ggataatgtc accggcttta ccggcggttc
    44941 acgggtgaac agtggaatga tgtttatcac ccttaaacca cgcggcgagc ggagcgaaac
    45001 ggcccaacag attatcgatc gcttgcgtaa aaaactggct aaagaaccgg gagcgaatct
    45061 gttcctgatg gcggtacagg atattcgcgt tggcggacgt caggctaacg ccagttacca
    45121 gtacacgttg ttatccgacg acctggcggc actgcgcgaa tgggagccga aaatccgcaa
    45181 aaaactggcg acattgccag agctggctga tgttaactcc gatcaggaag ataatggcgc
    45241 ggagatgaac cttatttacg atcgcgacac catggcgcga ttgggaattg acgtgcaagc
    45301 cgccaacagc ctgttaaata acgccttcgg tcagcggcaa atctcgacca tttaccagcc
    45361 gatgaaccag tacaaagtgg tgatggaagt ggatccgcgc tatacccagg acatcagtgc
    45421 actggaaaaa atgttcgtta tcaataacga aggaaaagcg atcccgctgt cgtatttcgc
    45481 taaatggcaa ccggcgaacg ctccactgtc ggtgaatcat cagggattat cggcggcctc
    45541 gaccatttcg ttcaacctgc cgaccgggaa atcgctctcg gacgccagtg cggcgatcga
    45601 tcgcgcaatg acccagcttg gtgtgccttc gacggtgcgc ggcagttttg ccggaacagc
    45661 gcaggtgttc caggagacga tgaactcaca ggtgatcctg attatcgccg ccatcgccac
    45721 ggtatatatc gtactgggta tcctttacga gagttatgtg catccactga cgattctctc
    45781 caccctacct tcggcgggcg tcggagcgct gttggcgctg gagctgttca atgccccgtt
    45841 cagcctaatt gcgctgatag ggatcatgtt attaatcggc atcgtgaaga aaaacgccat
    45901 tatgatggtc gattttgcgc ttgaagccca acggcacggt aacctgacgc cgcaggaagc
    45961 tattttccag gcctgtctgc tgcgttttcg cccgattatg atgactaccc tggcggcgct
    46021 gtttggtgcg ctgccgctgg tattgtcggg cggtgacggc tcggagttgc ggcaaccatt
    46081 ggggatcacc attgtcggcg gtctggtcat gagccagctt ctgacgctgt ataccacgcc
    46141 ggtggtgtat ctctttttcg accgtctgcg gatgcgtttt tcgcgtaaac ctaaacaagc
    46201 ggtaaccgag taaatgacag atcttcccga cagcacccgc tggcaattgt ggattgtggc
    46261 tttcggcttc tttatgcagt cgctggacac caccatcgtt aacaccgcgc ttccctcaat
    46321 ggcgcaaagc ctcggggaaa gtccgttgca tatgcacatg gtcattgtct cttatgtact
    46381 gaccgtggcg gtgatgctgc ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa
    46441 tattttcttt accgccatcg tgctgtttac tctcggttca ctgttttgcg cgctttccgg
    46501 cacgctgaac gaactgttgc tggcacgcgc gttacagggc gttggcggcg cgatgatggt
    46561 gccggtcggc aggttgacgg tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat
    46621 gacctttgtc acgctgcccg gtcaggtcgg gccgctgctt ggcccggcgc tcggcggtct
    46681 gctggtggag tacgcatcgt ggcactggat ctttttgatc aacattccgg tgggaattat
    46741 tggcgcgatc gccacactga tgttaatgcc gaactacacc atgcagacgc ggcgctttga
    46801 tctctccggc tttctgttgc tggcggttgg catggcggta ttaaccctgg cgctggacgg
    46861 cagtaaaggt acaggtttat cgccgctggc gattgctggc ctggtcgcag ttggcgtggt
    46921 agcactggtg ctttatctgc tgcacgccca aaataacaac cgcgccctgt tcagtctgaa
    46981 actgttccgc actcgtacct tttcgctggg cctggcgggg agctttgccg gacgtattgg
    47041 cagtggcatg ttgcccttta tgacaccggt tttcctgcaa attggcctcg gtttctcgcc
    47101 gtttcatgcc ggactgatga tgatcccgat ggtactcggc agcatgggaa tgaagcgaat
    47161 tgtggtacag gtagtgaatc gctttggtta tcgtcgggta ctggtggcga ccacgctggg
    47221 cctgtcgctg gtcaccctgt tgtttatgac taccgctctg ctgggctggt actacgtttt
    47281 gccgttcgtc ctgtttttac aaggaatggt caactcgaca cgtttctcct ccatgaacac
    47341 cctgacgctg aaagatctcc cggacaacct ggcgagcagc ggcaacagtc tgctgtcgat
    47401 gattatgcaa ttgtcgatga gtattggcgt caccatcgcc gggctgttgc tgggactttt
    47461 tggttcgcag catgtcagcg tcgacagcgg caccacacaa accgtcttta tgtacacctg
    47521 gcttagcatg gcgtctatca tcgcccttcc ggcgttcatc tttgccagag tgccgaacga
    47581 tacgcatcaa aatgtggcta tttcacggcg aaaaaggagc gcgcaatgaa gttctggcga
    47641 cccggtatta ccggcaaact gtttctggcg attttcgcca cctgcattgt cttattgatc
    47701 acgatgcact gggcggtacg tatcagtttt gagcgcggct ttatcgatta catcaagcat
    47761 ggtaatgaac aacggctgca aatgctcggc gatgcgcttg gtgagcagta cgcccagcac
    47821 gggaactggc gcttcctgcg taataacgat cgctttgtat ttcagatcct acgttcactg
    47881 gagcatgata acaacgaaga taagcccggc cccggtatgc cgccacacgg ctggcgcaca
    47941 caattttggg tggttgatca aaacaacaaa gtgctggttg gcccgcgagc accagttcca
    48001 cccgacggca cacggcggcc cattatggtc aatggtgcgg aagttggcgc ggtgatcgcc
    48061 tcccctgttg aacgactgac ccgtaatact gatatcaatt ttgacagaca acagcggcaa
    48121 accagttggc tgattgtggc tttatctacc ttattagcgg cgctggcgac attcccactg
    48181 gcgcgcggtt tgctggctcc ggtaaaacga ctggtggatg gcacgcacaa actggcagcg
    48241 ggtgatttca ctactcgcgt agcccccacc agcgaagatg agttgggcag actggcagag
    48301 gatttcaatc aactcgccag cacactggag aaaaaccagc agatgcgccg cgattttatg
    48361 gccgatattt ctcatgaact gcgcacgcct ttagcagtac tgcgcggcga actggaagcc
    48421 attcaggatg gtgtgcgtaa attcacgccg gagacgctgg cttctttaca ggcagaagta
    48481 ggtacactga caaaactggt tgacgatctc catcaattgt ctatgtctga tgaaggcgct
    48541 ctcgcctatc aaaaagcacc gatagatttg atcccactgc tggaagtggc gggaggcgca
    48601 tttcgcgaac gattcgccag ccgtggcctg aaattgcaat tttccctgcc agacagtatt
    48661 accgtatttg gcgatcgcga tcgtttaatg cagttgttca ataacttact ggaaaacagc
    48721 ctgcgctaca ctgacagcgg cggcagcctg aaaatctctg ccgagcagca cgacaaaacg
    48781 gtgcgcctga cctttgccga cagtgcgccg ggtgtcagtg acgatcagct acaaaaattg
    48841 tttgaacgtt tttatcgcac cgaaggttcc cgcaaccgcg ccagcggtgg ttccgggctg
    48901 gggctggcga tttgcctgaa cattgttgaa gcacataacg gccgcattat tgccgcccat
    48961 tcgccttttg gcgggcttag cattacagta gagttaccgc tggaacggga tttacagaga
    49021 gaagtatgac cgagttacca atcgacgaaa acacaccgcg tattttgatc gtggaagatg
    49081 aaccgaagct ggggcagttg ctcattgatt atctgcgtgc tgcgagctat gcgccaacgt
    49141 tgattagcca cggcgatcag gtactgccat atgtgcgcca gacaccaccg gatctgatcc
    49201 tgttggatct gatgctccct ggcaccgatg gcctgacgct gtgccgggaa attcgtcgtt
    49261 tttctgacat tccgatcgtg atggtgacgg caaaaatcga agagatcgac cgcctgctgg
    49321 ggttggagat tggcgcagat gactatatct gcaagccgta cagcccacgg gaagtggtag
    49381 cgcgcgtcaa aaccattttg cgccgttgca aaccgcagcg tgagttgcag caacaggatg
    49441 ctgaaagccc gttgattatc gacgaaggtc gttttcaggc ttcctggcgc ggtaaaatgc
    49501 ttgacctgac gcctgcagaa tttcgactgc tgaaaacgct ctctcatgaa ccaggaaaag
    49561 tgttctcccg cgagcagttg ctgaaccatc tttatgacga ctaccgcgtg gtaaccgacc
    49621 gtaccatcga cagccacatt aaaaacctgc gccgcaagct ggaatctctc gacgccgaac
    49681 agtcatttat ccgcgccgtt tacggcgtcg gttaccgctg ggaagccgac gcctgccgca
    49741 tcgtttaaca tttttacctg ccggatctgg ccttcagacc agatccggca acattatccc
    49801 acgcatagtc agcaaactga catgatctct ccgccctggc aggcaccaga aaatcgctca
    49861 tactttaatc ggcaaacagt ccttttagat gctgttttcg atacgcaatt tcaatcaagg
    49921 agtcattatg gctggttggt ttgaactcag taagagcagt gataatcaat tccggtttgt
    49981 gctaaaagcg ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc
    50041 tgcggaaaag ggcatcgcgt cggtacgtag caacagcccg caagaagaac gctatgagaa
    50101 aaaaacggca agcaacggca aattctattt caatctgaaa gccgccaatc atcaaattat
    50161 cggctccagc cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa
    50221 agcaaatggc acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtaggt
    50281 tgttggcgtc cggcgcttta ccagttacct ccacagcgct acaatgcccg cccttaaagt
    50341 gggggcactc ccctgaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag
    50401 agaattatgt ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac
    50461 gctttcgctt atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctgcgtgtg
    50521 cgcaacaacg aattcaacca cgaaaatctt caactcggca ttaacgaagc ccacgcgctg
    50581 gggaaaaagt tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt
    50641 atccgtgacc tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca
    50701 gggctgatta tgctggtgcg tgagcacttc cctgaaatgc cgatccatct ctcggtacag
    50761 gctaacgccg tgaactgggc gacggtgaaa ttctggcagc aaatgggcct gacccgcgtg
    50821 atcctctctc gcgaactgtc actggaagag attgaagaga tccgcaatca ggtgccggat
    50881 atggaaatcg aaatcttcgt tcacggcgcg ctgtgcatgg cctactccgg tcgctgcctg
    50941 ctctctggct atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc
    51001 tgggagtaca acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac
    51061 gagccgattc cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa
    51121 gtgtttatga tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag
    51181 cacggcactt acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt tgaacgcctg
    51241 accaaaatgg gcgtgcattc gctgaaaatc gaaggccgta ccaaatcttt ctactattgc
    51301 gcacgcaccg cgcaggttta ccgtaaagct atcgatgacg ccgctgcggg caaaccgttc
    51361 gataccagcc tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc
    51421 ctgcgtcgtc atactcacga cgattatcag aactacgaat acggttattc agtttctgac
    51481 cgccagcagt ttgttggtga gtttaccggt gagcgcaagg gggacctcgc ggcggtagcg
    51541 gtgaaaaata aattctccgt tggcgacagc cttgagctaa tgacgccgca aggcaacatt
    51601 aactttaccc ttgagcacat ggaaaacgcc aaaggtgaag ctatgccggt cgcaccaggc
    51661 gatggttata ctgtgtggct cccggtgccg caggatcttg agctaaatta cgcgctgctg
    51721 atgcgtaatt tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt
    51781 atttttcccg gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt
    51841 atcatccgtt tcgctgaaaa acataaccca taaaatgcta gctgtaccag gaaccacctc
    51901 cttagcctgt gtaatctccc ttacacaggc ttatttttta tgcataagcc ctatccctgg
    51961 tcaccgtctt ccattgacca catcgataga atctccctta atagcacgat gcctttcact
    52021 tatcgacatc gtgctcgcac aggttccggt tacgcacagc cagaacgcgc atgtttgacg
    52081 cttaccaaaa aatgaaccta aagcattgga atatttttga catcattttc tgatggctgc
    52141 ataaaataaa aattctgctt tagtttcatc tatctgtttg tcattattac tcacattcaa
    52201 taatggtgtt gaagaatatc ccatcacaga taaaataaat atgtgcatgg tagtcttgaa
    52261 tctattctca ctctccacat ttgaatgtca gacgagcaac gccatgtaat cctgcacctt
    52321 ctgtcttcaa tttaactatc tgcatttttt tgccctgagt aacacagaaa tgggctgcat
    52381 cattttttac tatattttct gcaccagata ttctgcccct ggctaaagaa gcttcggctt
    52441 cggtg
//
LOCUS       DAPQZD010000013        49835 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPQZD010000013 DAPQZD010000000
VERSION     DAPQZD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.013"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            28..1077
                     /locus_tag="RO454_000780"
     CDS             28..1077
                     /locus_tag="RO454_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDY0932657.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1151..2008
                     /gene="nfo"
                     /locus_tag="RO454_000781"
     CDS             1151..2008
                     /gene="nfo"
                     /locus_tag="RO454_000781"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDY0932658.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2012..3100
                     /locus_tag="RO454_000782"
     CDS             2012..3100
                     /locus_tag="RO454_000782"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0932659.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3368..4309)
                     /gene="rihB"
                     /locus_tag="RO454_000783"
     CDS             complement(3368..4309)
                     /gene="rihB"
                     /locus_tag="RO454_000783"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDY0932660.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4439..5137
                     /gene="yeiL"
                     /locus_tag="RO454_000784"
     CDS             4439..5137
                     /gene="yeiL"
                     /locus_tag="RO454_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDY0932661.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(5204..6454)
                     /locus_tag="RO454_000785"
     CDS             complement(5204..6454)
                     /locus_tag="RO454_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDY0932662.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6548..7486)
                     /gene="psuG"
                     /locus_tag="RO454_000786"
     CDS             complement(6548..7486)
                     /gene="psuG"
                     /locus_tag="RO454_000786"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDY0932663.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(7474..8415)
                     /locus_tag="RO454_000787"
     CDS             complement(7474..8415)
                     /locus_tag="RO454_000787"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDY0932664.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(8871..10562)
                     /gene="fruA"
                     /locus_tag="RO454_000788"
     CDS             complement(8871..10562)
                     /gene="fruA"
                     /locus_tag="RO454_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDY0932665.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(10579..11517)
                     /gene="fruK"
                     /locus_tag="RO454_000789"
     CDS             complement(10579..11517)
                     /gene="fruK"
                     /locus_tag="RO454_000789"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDY0932666.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(11517..12647)
                     /gene="fruB"
                     /locus_tag="RO454_000790"
     CDS             complement(11517..12647)
                     /gene="fruB"
                     /locus_tag="RO454_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDY0932667.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            13015..14196
                     /gene="setB"
                     /locus_tag="RO454_000791"
     CDS             13015..14196
                     /gene="setB"
                     /locus_tag="RO454_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDY0932668.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(14193..14447)
                     /locus_tag="RO454_000792"
     CDS             complement(14193..14447)
                     /locus_tag="RO454_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDY0932669.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            14602..15174
                     /gene="yeiP"
                     /locus_tag="RO454_000793"
     CDS             14602..15174
                     /gene="yeiP"
                     /locus_tag="RO454_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDY0932670.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            15397..16863
                     /locus_tag="RO454_000794"
     CDS             15397..16863
                     /locus_tag="RO454_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDY0932671.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            16981..17967
                     /gene="yeiR"
                     /locus_tag="RO454_000795"
     CDS             16981..17967
                     /gene="yeiR"
                     /locus_tag="RO454_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDY0932672.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            18006..18719
                     /gene="lpxT"
                     /locus_tag="RO454_000796"
     CDS             18006..18719
                     /gene="lpxT"
                     /locus_tag="RO454_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDY0932673.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            19131..19697
                     /gene="mepS"
                     /locus_tag="RO454_000797"
     CDS             19131..19697
                     /gene="mepS"
                     /locus_tag="RO454_000797"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDY0932674.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            19878..21434
                     /locus_tag="RO454_000798"
     CDS             19878..21434
                     /locus_tag="RO454_000798"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDY0932675.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            21516..23330
                     /locus_tag="RO454_000799"
     CDS             21516..23330
                     /locus_tag="RO454_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0932676.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKISDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            23331..24425
                     /locus_tag="RO454_000800"
     CDS             23331..24425
                     /locus_tag="RO454_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDY0932677.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            24425..25450
                     /locus_tag="RO454_000801"
     CDS             24425..25450
                     /locus_tag="RO454_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932678.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            25452..27041
                     /gene="yejF"
                     /locus_tag="RO454_000802"
     CDS             25452..27041
                     /gene="yejF"
                     /locus_tag="RO454_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDY0932679.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(27045..27389)
                     /locus_tag="RO454_000803"
     CDS             complement(27045..27389)
                     /locus_tag="RO454_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932680.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(27723..28913)
                     /gene="bcr"
                     /locus_tag="RO454_000804"
     CDS             complement(27723..28913)
                     /gene="bcr"
                     /locus_tag="RO454_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDY0932681.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(28941..29636)
                     /gene="rsuA"
                     /locus_tag="RO454_000805"
     CDS             complement(28941..29636)
                     /gene="rsuA"
                     /locus_tag="RO454_000805"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDY0932682.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            29786..31546
                     /locus_tag="RO454_000806"
     CDS             29786..31546
                     /locus_tag="RO454_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDY0932683.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            31671..31955
                     /gene="rplY"
                     /locus_tag="RO454_000807"
     CDS             31671..31955
                     /gene="rplY"
                     /locus_tag="RO454_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDY0932684.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(32094..33101)
                     /gene="yejK"
                     /locus_tag="RO454_000808"
     CDS             complement(32094..33101)
                     /gene="yejK"
                     /locus_tag="RO454_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDY0932685.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            33283..33510
                     /locus_tag="RO454_000809"
     CDS             33283..33510
                     /locus_tag="RO454_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDY0932686.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            33530..35290
                     /gene="pbgA"
                     /locus_tag="RO454_000810"
     CDS             33530..35290
                     /gene="pbgA"
                     /locus_tag="RO454_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDY0932687.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            35365..35441
                     /locus_tag="RO454_000811"
     tRNA            35365..35441
                     /locus_tag="RO454_000811"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35399..35401,aa:Pro,seq:ggg)
     gene            complement(35552..36495)
                     /locus_tag="RO454_000812"
                     /pseudo
     CDS             complement(35552..36495)
                     /locus_tag="RO454_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            36815..37462
                     /gene="narP"
                     /locus_tag="RO454_000813"
     CDS             36815..37462
                     /gene="narP"
                     /locus_tag="RO454_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDY0932688.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(37497..38549)
                     /gene="ccmH"
                     /locus_tag="RO454_000814"
     CDS             complement(37497..38549)
                     /gene="ccmH"
                     /locus_tag="RO454_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDY0932689.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(38546..39103)
                     /gene="dsbE"
                     /locus_tag="RO454_000815"
     CDS             complement(38546..39103)
                     /gene="dsbE"
                     /locus_tag="RO454_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDY0932690.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(39100..41043)
                     /gene="ccmF"
                     /locus_tag="RO454_000816"
     CDS             complement(39100..41043)
                     /gene="ccmF"
                     /locus_tag="RO454_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDY0932691.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(41040..41519)
                     /gene="ccmE"
                     /locus_tag="RO454_000817"
     CDS             complement(41040..41519)
                     /gene="ccmE"
                     /locus_tag="RO454_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDY0932692.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(41516..41725)
                     /gene="ccmD"
                     /locus_tag="RO454_000818"
     CDS             complement(41516..41725)
                     /gene="ccmD"
                     /locus_tag="RO454_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDY0932693.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(41722..42459)
                     /gene="ccmC"
                     /locus_tag="RO454_000819"
     CDS             complement(41722..42459)
                     /gene="ccmC"
                     /locus_tag="RO454_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDY0932694.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSILGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(42501..43163)
                     /gene="ccmB"
                     /locus_tag="RO454_000820"
     CDS             complement(42501..43163)
                     /gene="ccmB"
                     /locus_tag="RO454_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDY0932695.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(43160..43777)
                     /gene="ccmA"
                     /locus_tag="RO454_000821"
     CDS             complement(43160..43777)
                     /gene="ccmA"
                     /locus_tag="RO454_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDY0932696.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(43796..44398)
                     /gene="napC"
                     /locus_tag="RO454_000822"
     CDS             complement(43796..44398)
                     /gene="napC"
                     /locus_tag="RO454_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDY0932697.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(44408..44857)
                     /gene="napB"
                     /locus_tag="RO454_000823"
     CDS             complement(44408..44857)
                     /gene="napB"
                     /locus_tag="RO454_000823"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDY0932698.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(44854..45717)
                     /gene="napH"
                     /locus_tag="RO454_000824"
     CDS             complement(44854..45717)
                     /gene="napH"
                     /locus_tag="RO454_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDY0932699.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(45704..46399)
                     /gene="napG"
                     /locus_tag="RO454_000825"
     CDS             complement(45704..46399)
                     /gene="napG"
                     /locus_tag="RO454_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDY0932700.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(46406..48892)
                     /gene="napA"
                     /locus_tag="RO454_000826"
     CDS             complement(46406..48892)
                     /gene="napA"
                     /locus_tag="RO454_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDY0932701.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(48889..49152)
                     /gene="napD"
                     /locus_tag="RO454_000827"
     CDS             complement(48889..49152)
                     /gene="napD"
                     /locus_tag="RO454_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDY0932702.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(49142..49636)
                     /gene="napF"
                     /locus_tag="RO454_000828"
     CDS             complement(49142..49636)
                     /gene="napF"
                     /locus_tag="RO454_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDY0932703.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        12182 a        13394 c        12269 g        11990 t
ORIGIN      
        1 ccttatgccc ggttaaagga gagggttatg acgaatatca ccttacagaa acaacatcgt
       61 acactgtggc attttattcc ggggttagcc ctgagtgcag ttatcaccgg ggtcgccctg
      121 tggggtggtt ccattcccgc ggttgcgggt gccgggttta gtgccctcac cctcgcaatc
      181 ttgttgggga tggttttagg caacaccatt tacccgcaca tctggaaaag ctgtgacggt
      241 ggtgtgctgt ttgccaagca atatttatta cgtctgggta tcattcttta tggcttccgt
      301 ctgacgttct cgcaaattgc cgatgtcggt atcagtggga tcatcattga cgtgttgacg
      361 ctgtccagta ccttcctgct ggcttgcttc ctggggcaga aagtgtttgg tctggataag
      421 cacaccagct ggttgatcgg tgccggtagc agtatctgtg gtgctgccgc ggtactggcg
      481 actgagccgg tagtgaaagc agaagccagt aaagtaaccg tggctgttgc aaccgttgtt
      541 atcttcggga ccgtcgcgat tttcctctac ccggcgatat atccgctgat gtctcaatgg
      601 tttagtccgg aaactttcgg tatctacatt ggttctactg tgcacgaagt ggcgcaggtg
      661 gtggcggcag gtcatgccat cagcccggat gcagaaaacg cagcagttat ttccaaaatg
      721 ctgcgcgtga tgatgctggc tccgttcctc atcctgctgg cggcgcgtgt taaacagctg
      781 tctgggacga acagcggcga gaaaagcaaa atcactattc cgtggtttgc catcttgttc
      841 atcgtcgttg ccatctttaa ctcgttccac ctgttaccgc agagcgtggt gaacatgctg
      901 gtaacgctgg ataccttcct gctggcaatg gcgatggcgg cgctgggtct gaccactcac
      961 gtcagcgcgc tgaaaaaagc cggggcgaaa cctctgctga tggcactggt actgtttgcc
     1021 tggctgattg ttggtggtgg tgcgattaac tatgtgattc aaagcgtcat cgcataaacc
     1081 actacatctt gctcctgtta acccgctatc attaccgttt tcctccagcg ggtttaacag
     1141 gagtcctcgc atgaaataca ttggagcgca cgttagtgct gctggcggtc tggcaaatgc
     1201 cgcaattcgc gccgccgaaa tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg
     1261 tcagtggcgt gccgcaccgc tcacgacgca aaccatcgat gaattcaaag ccgcctgtga
     1321 aaaatatcac tacacatcgg cgcaaattct tccccacgac agttatctga ttaacctcgg
     1381 acatccggtt gctgaagctc tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg
     1441 ttgcgaacag ctggggcttt ctttgctcaa cttccaccct ggcagccatc tgatgcagat
     1501 ttcagaagag gattgccttg cgcgtattgc cgaatccatc aacattgcgc tggataaaac
     1561 tcaaggtgtc accgcggtga ttgaaaacac cgccggtcag ggcagtaact tagggtttaa
     1621 attcgaacat ctcgcggcga ttatcgacgg cgtggaagat aaatcccgcg tcggcgtctg
     1681 cattgatacc tgccatgctt tcgctgccgg gtatgatttg cgtactcctg ccgaatgcga
     1741 gaaaacgttc gctgattttg cccgtattgt aggctttaag tatctgcgcg ggatgcacct
     1801 caacgatgcg aaaagcacct ttggcagccg cgttgaccgt catcatagcc tcggcgaagg
     1861 caatatcggt catgatgcgt tccgctggat catgcaggac gaccgtttcg acggcattcc
     1921 gctgatcctt gaaaccatca acccggatat ctgggcagaa gagatcgcct ggctgaaagc
     1981 gcaacaaact gaaaaagcgg tggcctgatc gatgaacgac agggaaaaac agattcttaa
     2041 aattttacgg cgtaacccgc tgattcaaca gaatgaaatc gccgatattc tgcaaatcag
     2101 ccgctcacgt gttgccgctc atattatgga tttgatgcgc aagggtttga ttaaaggcaa
     2161 aggatatatc cttaccgagc aggattactg tgtggttgtg ggtgcaatca atatggatat
     2221 tcgcggaatg gcggatatcc gttacccgca agcagcctct catccaggtt ctgttcactg
     2281 ttccgcaggc ggcgtcggac gtaatatcgc gcataacctg gctctgcttg gtcgggatgt
     2341 gcatttaatt tcagctattg gcaacgattt ttacggcgag acattactgg aagaaacgcg
     2401 ccgcgcgggc gtgaacgttt caaactgcat tcgtctgcat ggacacagca ctgcaactta
     2461 tctcgctatc gctaataagc aggaagaaac tatcctggca atcaatgaca cacacattct
     2521 gcaacagcta acgcctcaac tgctcaatac ttcacgggac ttgattcgcc atgctggtgt
     2581 ggtgctggct gattgtaatc tcacacctga agccctggag tgggtcttta ccattgctga
     2641 tgaaattccg atgtttgtcg ataccgtttc cgaatttaaa gcgaacaaag taaaaaactg
     2701 gtacagccgg atccacactc tgaaaccaac acaaaatgaa ctggaaattt tgtgggggca
     2761 accgattaaa gacgacaacg atcgcatcag agctgtaaat tcgctgcatc aacaaggtgt
     2821 taaacgcatt tttgtttatc tgaaagatga atctgtattt tgcagcgata aggatggaga
     2881 acaatttttg ctgaccgcac cagcgcatac gacagtagac agttttggtg ctgacgatgg
     2941 ttttatggcg ggcctggtat atagctttct tgaaggatac agtttccgcg acagcgcccg
     3001 ttttgcgata gcctgcgcgg caatttcgcg tgccagcggc agcttaaaca accctaccct
     3061 gtctgccgat aacgcgcttt cattagtgcc aatggtgtaa caatgttgcc ggattcgacg
     3121 cttgaccgca tcttatccgg ctcataaatt gcgcactacg ctaaaccaat aaagaatcct
     3181 gcaatagtag cactcatcag gttactgaca gcaaaaacgc aatagccagc aataccacca
     3241 ttcccagaac acttctcatg acatccatag ttattatctc ttcatgtcat tttgggcgac
     3301 ggctctctgc ccatcataaa tagccgggcc tgcatcttgc gatactgacc cggcttgtta
     3361 tcaacactta atgggttttg atgtagccgc gtacgcactc ttcgactaat ccccagaacc
     3421 agtctgtatc aatagtgatg ccgactttgg tattggcggg cttgccaaga acgcccaact
     3481 cgtcgcagac ggtacgccca tagcaagggc cactgttaac gtccacttcg acgtacatct
     3541 cctgggtttt aatgccatca gggttaatca aataaccgat gcaggtggcg tcgtgcaccg
     3601 ggccgccagc caggccgtag ttttcgaact gcgttttgag agtgaagttc atgatgtcgc
     3661 tgaacagctc tcccgcaggg ccgccagccc tttccatccg agcaatcacg tccggggtgc
     3721 aaacggtctg gttggtaaga tcgaggccca tcatcactaa tggaacgccg gaggtgaaca
     3781 ccacgcgtgc ggcttccggg tcagcaaaga tattgaactc agcagatggg gtgaagttgc
     3841 cagtaccata agcgccgccc atcagaacaa tttcacggat tttcggcagg atcgcgggtt
     3901 gcatacgcat tgccaccgcg atgtttgaca acggaccaac cggcaccaga gtgatatcgc
     3961 catcgcttgc cataagggta tcgatgatat atttcaccgc atgagtgttt tctgcctggc
     4021 gggtcagcgg ctcgaatacc gggccatcca gtccggtttc accgtgaata ttatcggcaa
     4081 caatttgttg acgcataatg ggctgcggca tccccgcata aaccggaaca ttaatctcca
     4141 gtttctggca aacattcagg ccattaatta atgttttatc gagggtttga ttacccgcta
     4201 caatggtgat acctaataaa tcaatcgcag gatgtttcgc cgccatcatt atagcaatag
     4261 catcatcatg acccggatca caatccagaa taatttttct cttttccatt gtttatttcc
     4321 tctgtttcca gttgcgttat tgtttctaca gcaaagaaaa ttcgacctgg ccgatgattg
     4381 aatcttaaca acagcgtacg tatgctaaat atgagaaatc tcatagcgga taaacatcgt
     4441 gaaagaaatc cacaataatg atcttaagca gcaattgatg agtgaatctg cgtttaagga
     4501 ttgcttttca acggatgttt cagccgatac gcggctgttt cattttttag cgcgtgacta
     4561 cattgtgcag gaagggcaac agccgtcctg gctgttttac ctgacgcgag gccgcgccag
     4621 gctttacgtc acgcttgcta atggtcgcgt gtcgctgatc gatttctttg ccgcgccctg
     4681 ttttattggc gagattgagt taatcgacaa agaccatgaa ccgcgtgcag tgcaggctat
     4741 tgaagagtgt tggtgccttg cgctccctat gaagcattac cgcccgctgt tattaaacga
     4801 cacgctattt ttacgaaaac tctgcgtcac cttaagtcat aaaaattatc gtaatattgt
     4861 ttctttaact cagaatcaat catttccgtt agtgaatcgc ctggcagctt ttatattact
     4921 ctcgcaggaa ggtgatcttt atcacgaaaa gcatacgcaa gcggcagagt atttaggcgt
     4981 ttcttatcga catcttttat atgttctcgc gcaattcatt aatgacggtt tattaattaa
     5041 aagcaagaaa gggtatctca ttaaaaacag aaagcagttg tcaggactgg cgctggagat
     5101 ggacccggag aataaattct ccgggatgat gcagtaaaaa ttattggcaa tagtgcgatt
     5161 gccggatgca acgcttaata aggcgtacat ccggcaatga aactcaagct aaaccaataa
     5221 agaacccggc aatggtcgcg ctcatcaggt tggaaagcgt cgccgccgcc agcgcccgta
     5281 aaccaagctg ggcgatttcc ggcgcgcgtt ttggcgaaat agccgaaaac gctccaacga
     5341 caacaccgat agaaccaaag ttagcaaacc cacagagggc gaacgagata atggcaatgg
     5401 tcttgacatc cagcgtgccg ctcgtttgca gatagggcga gaaattgaga taagcaacaa
     5461 actcattaat cgccaatttc tgcccaatca aacttccggc aagatttgca tcactccagt
     5521 ccacccccat aatccatgcc aacggggcca gcacatagcc gaaaatgctt tccagagaga
     5581 cattggcaaa accaaaccag ccgccaactc caccaataat gccgttgatc agcgcaatga
     5641 tcgccacaaa tgccattacc accgtcgcca caccggcggc gattttcagc ccggtcattg
     5701 caccgttcgc cgcagcttca ataatgcttt ttggcggtgt ttcgctaaac gacagatttt
     5761 caaacgtaac ttgcgaaggt tccgtcgccg gactaagaat acgtgcaaac aggataccgc
     5821 caggaatagc catcagcgaa gcggctaaca aatagtcaat cggcacgccc attccggcat
     5881 agccaatcat catcgaaccc gcaatagacg ccatgccgct acaaatagcc gtaaatagct
     5941 cgttgcgatt catgcgatca ataaaaggtt taacgatcgc cgggatctca ttttgcccga
     6001 ggaagatagt ggtgactgcg acaaaagatt cgattttgct gatgttgagg gctttctgaa
     6061 aaatactgcc aagaatacga atcagcagcc ccattacgcc aatgtagtac agcaagctga
     6121 taagcgcggt aacgaaaata atcgccggga gtacgcgaaa ggcgaagata aagcccgcac
     6181 cgtcaaacag aacatccatt ttgggaccga ccagcgaacc aaaaataaag gcacttcctg
     6241 cgtcactgta ggacatcact ttatggacgc ctaatgccgc ctgctctact gcccattttc
     6301 ccgggggaaa atagagcatg atgccaccaa tggcaatttg cagcagcagc gcagccccaa
     6361 ccgtgcgcaa gctgatgctc tttttattta ccgacaacac aaaggcaatt gccagcaata
     6421 ccaccatccc cacaacactt ctcattatat ccataatgat tttcccttca tgccagtaaa
     6481 cccggcgtca gcgccaggtt ttggtatgtt tgatgagtac gggcgacggc tttctgccca
     6541 ccagaaatta acccacgaga cgctgatatt ctttggcaat ttcgctcgcc agaatggcgt
     6601 tattaaagac cagttgaata ttggatttca ggctatcacc gccggttagc tccgcaacac
     6661 gcgccagcag gaacggtgta ctctctttcc caataacacc ctgttcttcg gcttcagcaa
     6721 cggcttgatc aattgctgca ttgatggttt cttctggcat cgcaaactgt tccgggatcg
     6781 ggttcgctac caccaggccg ccgttcaaac cggtttgcca cttcactgcc atcgcgcgag
     6841 caatttcgcg ggcactgtcg agacgaatgc tgacatcaaa tgggctggtt cggcagaaaa
     6901 acgcaggcag cgcttttgtc tgataaccaa ttaacggtac accgaaggtt tctaaatatt
     6961 ctgttgttaa tcctaaatcg agaatagatt ttgcccccgc acaaacaaca gtgacattgg
     7021 tatttgccag ttcctgtaaa tcggcagaaa tatcgaaggt atgttccgct ccgcgatgca
     7081 caccaccaat acctccggtt gcaaaaactt taatcccagc cagtgctgca ataatcatgg
     7141 ttgatgcaac ggtagtcgcg ccatttttcc ctgcagcaac gacaaaaggt aaatcacgac
     7201 gactaacttt cgtcacgtta tgtccttcac ggcccagcaa ttcgatttct tctttactta
     7261 agcccacttt catcacaccg ccaataatgg cgatagtggc tggtactgcg ccctgtttac
     7321 gaattgtttc ttctacttca attgcggttt gggcattttg tgggaatggc attccgtgag
     7381 aaattatggt cgattccagc gcaacaaccg gttttttatt ttttaaagca tcctgtactt
     7441 cagcggatat ttgtaataat tcaggggaca gagttaattc agacattctg tgttctccac
     7501 taacgatgta acattggcaa ttgataattc agggttgttg gtatattcac aggcaagtgc
     7561 cattgacgaa catccctgcg caaatcgaac agaatcgata aacggcatac cgtctaccca
     7621 acaggaggcc agccccgcca tcatggcgtc gcctgcccca gtaacattga tgacattcgt
     7681 tttaataggg gccgaccacc cactttcacc gttgatatca ctgtagtaaa cgccatcccc
     7741 tcccatgctc agcaccaggc ggttcaggcc gtgttgatga aaccaggcgg cgacctttgc
     7801 gacatcttca cgtccggaaa gtgcaattcc gctcaacgtt tcggcttcaa gacggttagg
     7861 ttttaacgta tggattttgc tcagatgatc gcggattttg acgcatttcc atgccgatac
     7921 cggatcaata aatacaggcg tttccccgct attttccaga acccagacca gcgcctcttc
     7981 actaaggttg caatccgcga caataacctt tgcaccgcga ataaactcct gatgctgggc
     8041 aagaaattct gcagaaatac agtcactaat actcatatca tttatcgcga caagcatttc
     8101 tcctgtatta tcgagcagtg ataaatagct ggatgtattc tcacccggaa caattaagca
     8161 tttatcgaca taaacaccgg attgattagt ttgcgccagt aatgattgcc cataaaaatc
     8221 accacctact gcacttaaaa gccaggcatt tttccccagt aacgccagat tatgagcgat
     8281 attccttccc acgccacctg gggtaaattt aatttttccg ggattcgaat ccgcataatt
     8341 caatggatga tgtgaatacc cagccacatc aatattcgcc gagccaataa ttactatgta
     8401 gtccttttcg cgcatagcac gccccctggc aataaattgc caacgaagaa attgggtaaa
     8461 atagtcatta cagtcaataa gactattaga gcatgtgttc agatttaaac acatgttcat
     8521 aataagcatg tttaacgtaa tgtaaatcga tcagaaaaaa cgaaatcggc cagatatgaa
     8581 ggcggtcaca aaatttgtct atttatgcag caacaaaaga gaaagttgtg atgtgtaaca
     8641 cacaatatgg caggcaagaa aaagggcaga gatgtttctg ccctcagatt gttgacaatg
     8701 cgcgctttgt tcatgctgga tgtggcgtaa acgccttatc cgacctacaa aagcgcaaaa
     8761 attcaatata ttgcagggtt catgtaggtc tgataagcat agcgcatcag gcaattttgc
     8821 gtttgtcatc atcggggcag agatgtctca tccccgatga tgaagagaaa ttatgcgttt
     8881 ttttctacta tttgtgtttc cgggcgtttg aggaaggcat aggccaaacc cgccaccagc
     8941 gtaccggcaa taattgctac caggtaaccc aataccggcg taatagcgcc agggatcagc
     9001 agaacaaaca gaccaccgtg cggagccatc agtttcgcac caatcgccat tgagattgcg
     9061 ccagtcagcg ccccaccaac gatacagcac ggtagcacac gcatcggatc acgagcagca
     9121 aacggaattg caccttccga aataaagcac agaccaagta ccagagcggc tttgccacct
     9181 tcctgctgcg ctttatcgaa tttacgacgc gccaccattg ttgccagacc cattgccagc
     9241 ggtggcacca tacctgccgc cataatcgcc gccatcgggc cataggtttg agtactcagc
     9301 agacccacac cgaatgcgta cgctgctttg tttaccggac cgcccatgtc agtacacatc
     9361 atgccaccga ggatcgcccc cagcagaacc gcattcgcag tccccatggt ctgcagccag
     9421 tgagtcaacc catcgagaat gccagcaacc ggtttaccga ttaggtagat catcgccaga
     9481 ccgaccacca gactggaaat tagcggaatg atcaggatcg gtttcagtgc ctccatactc
     9541 tgtggcagtt tcagttgcgt actgattaac ttcgcaatgt aacccgccag gaagcccgca
     9601 ataataccgc caatgaagcc agaaccggtg ctaaccgcca gcataccgcc aatcagaccc
     9661 ggcgtgagac ccggacgatc ggcaatggag aaggcaatat aacctgccag taccggcacc
     9721 atcagcgcaa aggctgaacc accaccaatc tgcatcagcg ccgctgccaa cgtacccggc
     9781 tctttaaacg cttcgatacc aaaagcaaaa gaaagcgcga tacacagacc acctgcaaca
     9841 accatcggca gcatgtaaga gacgcccgtc agcaagtgac ggtaagcgcc tgcactctct
     9901 ttcttacctt cagtggtcgc tgtttgagct ttgcccgctg gttcatacgg cgttgcttca
     9961 gcaaccgctt tatccagttc ctgcgcagtt ttcttcagcg ccagaccggt agaggtgcga
    10021 tacatcggtt taccagcaaa tttcgccaga tccacttcga tatctgccgc cacaatcacc
    10081 agatccgctg ctgcgacttc ttcaggtgtg attgcattac ccgcgccaac agaaccacgg
    10141 gtttcaactt tcacccacca gccacgtttt ttcgcttcgg tttcaatggc ttcagccgcc
    10201 ataaaggtgt gtgctacacc agtcgggcaa gcagtcaccg caactacgcg tttcggaccg
    10261 ctagcagcaa ccggtgctgt cgcagcgacc ggcgcagtgt aaggtttcgc atggcctttg
    10321 gcttcactca ggaacagctc agggtgcgca actgcccggg aaatatcgcc cagccagaca
    10381 tttttaccgt tcagcgcgct gtcattcggg atgaaatcac cgagaacaat cgccatttct
    10441 gcgtcgttcg gattgtcgat gatttccagt tttgcttttc gcgccgccgc gcccagcagg
    10501 gtcttcgcca tataggcgcg tgcctgaccg agattagcgt caataatcag cagcgttttc
    10561 attatgcctc tcctgctgtc agttaaaagg ttgtaagtcg acgcgcgcca tcattgcggc
    10621 caactgcgga cgatcggtaa tacccacatt gctttgactt accgccaggg ctgcaacagc
    10681 tgtcgccaga cgcagtgtgt gttcactgga ttcacgcatc agcaagccat aaatcaggcc
    10741 accaaccata gaatcccctg cgccaacggt gcttacgaca tcgactgacg gtggtttggc
    10801 gatccattcg ccggaggcat taacccaaag cgcgccttcg gcacccagtg aaataacaac
    10861 atgcgcaata ccttgttcac gtagcgcatg tgcagcttca atcacatctt tcatttcagg
    10921 cagtttacgg cctgcccaga tttccagctc gcggcggtta ggtttcacca gccacggtgc
    10981 cgctttcaaa cctgctacta acgcttcacg gctactatca aagataatgc aaggacactg
    11041 actacgcagg cgagtcatcc agtcggtgaa cgcttccggg ctgacgcctg acggtaagct
    11101 tccgctgaca cagaccatat cgaactgacc gagccagctc agagaatcag tcacaaagcg
    11161 ttcccagtcg gcgggggtaa cttcaaaacc cgagaagttg aagtcggtca cttcgccgtc
    11221 tttttccgtc agcttaacgt taattcgggt gcgcccctgt acaacctgga aacggttggc
    11281 aatgcccagc tcgctgaaca gttgctgaaa accatcctga ttgtctttac ccaggaagcc
    11341 gccaacggtg acatcaattc ccaggtcttt taatactttg gccacgttga tgcctttacc
    11401 cgccgcatgc agaccggtgg ttttcaccag gttcacttcg ccgcgttcaa tttccgggca
    11461 gaaaccaaca aggtcataag ccggattaag ggtgatcgta gcaacacgtc tgctcattat
    11521 gcgccctccc caagaccagc agcgatagcg tcgccaatgg ctttcagcgc ctgttcagca
    11581 tctgcaccct gggcggtaaa gcgtaggcga tgacctttct taacgccaag tgccacaact
    11641 ttcatcagac tacgtccgtt tgccggtttg ccggtgccat caaggtttgt cacggtaata
    11701 tcactgttaa attgtttaat ggtattgacc agcatggtac ctggacgagc atgcaggccg
    11761 tgttcattgc gcaccacaaa ctccgcgctt aacacgtcgt cggtcggcgc atcatcgctg
    11821 gtcagcagcg ccagcaacgt tgccgcatcc gctttcagca agcggtcagc tttattatcg
    11881 agcaacaaat cagcgagacg cttaagaacc gcgatgggct gatcgtcatt cattgccaca
    11941 ctcaccagca tggctgccgt ttcgccgtcc acatcaaaag catttgccgc acggcttacc
    12001 gcaatcgcgc tacgcagatt gccttcggcg ctatcgctca gccagatacc ctgtccgagg
    12061 ttcagcggtt gttcattgat ggctttggtg acgaaagtgg cgtcaactgc ccccgcttct
    12121 ttcagacgcg cagcgttcag cgcctgaaga gtcagcagat cgctggcgac gatatccagc
    12181 gtcagcattt cgttgtcgag cttcagctgc tcactctgct tttcgcccat cagcaatgcg
    12241 cgaagttctt ctgctgttgt tgctgacttc agttgttcag caacggaatc atcgctcagt
    12301 acgtgggtca gctggcgtag caggcccaga tgttcatccg agctggcagc aataccgatc
    12361 gccacgtacg ctacctggcc gtccccccag gtgacgcctt ccgggaactg aaatacctga
    12421 acgccggttt tcagcacctg atcgcgggtg tcggtagtgc cgtgtggaat agcaatacca
    12481 ttgccgagga acgttgaggt ttgctgttcg cgcgccagca tgccattgac gtagccttca
    12541 gctacattac cggcctgcac cagcgccgca gcgacctggc gaatcgcctc ttctttgtct
    12601 ccggcctttt cgcccggatg gatgtcctgt acagataact ggaacatagt tctcctctct
    12661 tgctgaattg aaacgattca gcttctatga gaaaaaaagc gccaacctgg cttagggtta
    12721 aagacaagat cgcgctgaaa cgtttcaagg aagcataata cttctgtttc agcgcgcaag
    12781 gaaagccgta aagttatgag caaaagtttg atgtgctgca caattttttg tcatttttcc
    12841 taattgctga tgggaaaatc cgttatcagc aatttcattt cagcagtgtc agcagccaat
    12901 cagcaacagt gaaatggcat tttgattatt cgtggcgctt ttgatttcgc tgtctgttta
    12961 ttttctgaca agcagcgtaa actccgcgtc ttcctcttcc agtgatcgac cagcatgcat
    13021 aactccccca cagtcaccag cgcgaaatcg tttgacctga cctcgacggc gtttttaatc
    13081 gttgcctttc tcaccggtat tgcgggcgct ctgcaaaccc cgacactcag tatttttctt
    13141 actgatgaag tacatgcccg tccggcgatg gtgggattct tctttaccgg cagcgctgtc
    13201 attgggattc tggtgagtca gtttctcgcc gggcgctctg ataagcgcgg cgatcgcaaa
    13261 tcgctgattg tcttttgctg cctgttaggc gtgctggcct gcaccctttt tgcctggaat
    13321 cgcaactact ttgttttgct attcgttggc gtctttctta gcagctttgg ctcgaccgct
    13381 aacccgcaaa tgtttgccct tgcccgtgaa catgctgaca aaaccggacg tgaggcggtg
    13441 atgttcagct cttttttacg cgctcaggtt tcactggcat gggtcattgg cccaccgctg
    13501 gcttatgcct tagcgatggg tttcagcttt acggtaatgt atctgagcgc agcggtagcg
    13561 tttattgttt gcggcgtgat ggtgtggctg tttttgccgt cgatgcaaaa agagcttccg
    13621 ctggcgaccg gcacggttga agcgccgcgc cgtaaccgtc gcgatacgtt gctgctgttt
    13681 gtcatttgta cattgatgtg gggctcgaac agcctgtaca tcatcaacat gccgctattt
    13741 attatcaacg aactgcatct tcccgagaaa ctggccggtg tgatgatggg gaccgccgcc
    13801 gggctggaaa tcccgaccat gttgatcgcc ggatattttg ccaaacgtct gggtaagcgc
    13861 ttcttaatgc gcgttgctgc cgtgggtggc gtctgttttt acgcaggaat gctgatggcg
    13921 cattcacctg tcattctgtt gggcttgcag ctgctaaatg ctatttttat tggcattctt
    13981 ggcggcatcg ggatgctcta ttttcaggat ttgatgcccg gtcaggcagg ttcagccacc
    14041 acgctctata ccaacacttc gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc
    14101 gtcgccgaga tctggaatta tcacgctgtg ttctggtttg cgatggtgat gattatcgcc
    14161 actctgtttt gcttactgcg aattaaagat gtttaaggcg cggtcaacat ttccagatcg
    14221 ataagccaga tcatggcctg ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag
    14281 cctgcacaga ctttcggtcg cagtggcgag gtgaaaattt tacagcgttg ctgttcatca
    14341 agctgaatgc agggagtatt ggcgggcttg ccatctggca taccgggaat agggctggaa
    14401 attgaagggg cagtgcaaca cgccccgcaa cccggacggc attccatacg ttgttctctt
    14461 aaggttaacc accggattga tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt
    14521 gcgaatacca cttgcccgaa aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt
    14581 tttgactaca ggaatttttc gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg
    14641 aattacaacg gcaaactgct gctggttaag gacattgata ttcagtcgcc cactgcccgc
    14701 ggtgccgcta cgctgtacaa aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa
    14761 gagcgtttca aaggtgatga tatcgttgac accgtgacgc tgacccgccg ttacgttgac
    14821 ttctcctatg tcgatggcaa cgaatatgtc tttatggata aagaagacta taccccgtat
    14881 accttcacca aagatcagat tgaagaagag ttgctgttta tgcctgaggg cggcatgccg
    14941 gacatgcagg tgctgacctg ggacggtcag ttgctggcgc ttgagctgcc gcagaccgtt
    15001 gatctggaaa tcgttgaaac tgcaccaggt atcaaagggg catctgccag cgcccgtaac
    15061 aaaccggcga cattgagcac tggtctggtg attcaggtac cagaatactt aagcccgggc
    15121 gaaaaaattc gtatccatat cgaagaacgc cgttatatgg gtcgtgctga ctaacttcag
    15181 ccgcatgcag aaaagggata gctcaggctg tccctttttt aatttattat accaatcttc
    15241 tattttgcgc ttcctgaaca ccttacgccc gcaattttca ctcgttgatt gatcgccctc
    15301 acacttcatc gcattaacaa tccagaccaa tttcaattgc tgtcatataa ctttacactg
    15361 tcattgttaa ttaatcgtta ctaagacgtg actcctatga atacaatcgc ctccgttacg
    15421 ctcccgcatc atgtacacgc tccacgctac gatcgacagc agttgcaatc acgtatcgtt
    15481 cattttggct ttggagcctt tcaccgcgca catcaggcat tactgaccga tcgtgtgctg
    15541 aatgcccagg gcggcgactg ggggatctgt gaaatcagct tgttcagcgg cgatcaactg
    15601 atgagccagc tccgcgcaca gaaccattta tataccgtgc tggagaaagg tgcggacggc
    15661 aatcaggcga taattgtcgg tgccgttcac gaatgtctga atgcaaaact ggattcctta
    15721 gcggcaatta ttgagaaatt ttgcgagcca caggtggcaa ttgtttccct gacaattacc
    15781 gaaaaaggct attgtattga cccggccacc ggtgcactcg acaccagcaa tccgcgtatt
    15841 attcacgatc tacaaaaccc tgaagaacct cactccgcgc caggtattct cgtcgaagca
    15901 ctgaaacgcc gccgtgagcg cggccataca ccgtttaccg tgctctcctg cgacaatatt
    15961 cccgacaatg gccatgtggt gaaaaacgcg gtgctgggaa tggcagaaaa acgttcgcca
    16021 gaactcgccg ggtggataaa agaacacgtc agttttccgg gaaccatggt cgaccgcatt
    16081 gttccggctg caacaaacga atcactggcg gaaataagcc agcatctggg ggtgaatgat
    16141 ccctgcgcga ttagctgcga accgtttatc cagtgggtgg ttgaagataa cttcgtcgct
    16201 gggcgtcctg cctgggaagt cgcaggtgta caaatggtga atgatgtcct gccatgggaa
    16261 gagatgaaac tgcggatgct taatggcagc cactcttttc tcgcttatct gggttacctc
    16321 tcaggattcg cccatatcag tgattgcatg caggatcgcg catttcgcca tgccgccaga
    16381 acattaatgc tggatgagca agcgccgaca ctgcggatta aagatgtcga tttaacacaa
    16441 tatgcggata agttaattgc acgttttgct aatccggcac tgaaacataa gacctggcaa
    16501 atcgcgatgg atggcagcca gaaattaccg caacgcatgt tggcaggtat tcgcatacat
    16561 ctggggcgcg aaacggactg gtcgttgctg gcattaggcg ttgcaggctg gatgcgttac
    16621 gtcagcggcg ttgatgatgc cggaaatgcc attgatgttc gcgatccgct tagcgataaa
    16681 attcgtgaac ttgttgcagg cagcagcagt gaacaacgcg taaccgccct gctttccctg
    16741 cgtgaaattt tcggtgatga tctgccagat aacccgcatt ttgtgcaggt catcgaacaa
    16801 gcctggcaac aaatcgcaca attcggcgcg catcaggcgc tattaaacac cctcaaaatt
    16861 taacgatttc tgcggttaaa gcggatgaag ctcgccttcg tccgctctcc ccttctcttt
    16921 tctgcctttt ttagccagga ttaacgctca gttaacttac cagagtattg cggagccgtt
    16981 gtgaccagaa ccaacctcat caccggtttt ctcggcagcg ggaaaactac gtcgattctt
    17041 catctgttag cccataaaga tcccaacgaa aaatgggcgg tactggttaa tgaatttggg
    17101 gaagtcggaa ttgatggtgc tttgctcgcc gatagcggcg cactgctgaa agagatcccc
    17161 ggcggttgca tgtgctgcgt taatggttta cccatgcagg tagggttgaa taccttactg
    17221 cgtcagggaa aaccagaccg cttgttgata gagccgaccg ggctgggcca tccgaaacag
    17281 atcctcgatc tcttaaccgc gccagtctat gaaccgtgga tagatctgcg cgccacgttg
    17341 tgcattctcg atccacgcct gctgctggac gaaaaaagcg ccagcaatga aaacttccgt
    17401 gaccagctgg ctgccgcaga cattattgtc gccaataaat ccgaccgtgc gacgcccgaa
    17461 agtgagcaag cgctagagcg ctggtggcag caaaatggtg gcgatcggca attaattcac
    17521 agcgagcatg gaaaaattga cggtcatctt ctggatttgc cgcgtcgcaa tttagccgag
    17581 ttgcccgcca gcgccgcgca ttctcatcag catagcgtga aaaaagggtt agcagcgtta
    17641 agcctgccag agcatcaacg ctggcgtcgc agtctgaaca gcggacaagg gcatcaggcc
    17701 tgtggctgga tattcgacgc tgatacggta ttcgacacca ttggtattct ggaatgggcg
    17761 cgacttgcac cggtggaacg cgtcaaaggc gtgctgcgta ttcccgaagg gctggtgcga
    17821 atcaaccgtc agggcgatga cctgcacatt gaaacgcaaa acgttgcgcc accggacagt
    17881 cgtattgagc tgatttccag cagcgaagct gactggaatg ccttgcagag cgcgctgttg
    17941 aagcttcgtt tagcgactac cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag
    18001 aaaatatgat taaaaatttg ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt
    18061 ttctttcctg gtatattccc attaatcatg gattctggtt gccgattgat gcggatattt
    18121 tttatttctt taatcagaaa ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga
    18181 ccaacaatcg cgctttcgac ggttgttcac tgctggcgat gggaatgttg atgctgagtt
    18241 tctggctgaa agaaaacgcc ccaggcagac gacgtatcgt gattattggt ctggtcatgc
    18301 tattaactgc agtggtatta aaccagctgg gtcaggcatt aattcctgta aaacgggcca
    18361 gtccaacgtt gacttttacc aatattaacc gcgtcagcga actgctctct gttcccacga
    18421 aagatgcctc acgagatagc tttcccggcg atcacggtat gatgctgctt attttttcgg
    18481 cattcatgtg gcgttatttc ggcaaagttg ctggccttat cgcccttatt atttttgtgg
    18541 tttttgcgtt tcccagagta atgattggcg cacactggtt tactgacatc atcgtcggtt
    18601 ccatgaccgt gatattgatc ggtttgccct gggtgttgct gacgccatta agtgatcgat
    18661 taatcacctt ttttgacaaa tcactacctg gaaaaaacaa acatttccaa aacaaataac
    18721 tcacagtaat taacatcatc aggtttattt ttatagtgag gataatcctg atgatgcgcc
    18781 tcctgctttc atccatcgag cgcaaaaatc attctctaag taaatgaatg gattgcatgc
    18841 gtttcactca atcgtacttt aattgaccaa tcccgctgat taactttctg tatcactttt
    18901 tcttataaaa aatcatgtaa aaccgctcgc caagaccgca ccaatcgggt aatctcgaac
    18961 tcgttttgcc tcggcggtag attatcctca cagcatataa ttttgtgcgt tagtccacag
    19021 atttggcctt aaggaattgt ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat
    19081 ttttttataa cgatatttgt cgttaaggac ttcaagggaa aacaaacaac atggtcaaat
    19141 ctcaaccgat tttgagatat atcttgcgcg ggatccccgc gattgcagta gcggttctgc
    19201 tttctgcatg tagtgcaaat aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg
    19261 gtagtgaaac atcatcactg caagcttctc aggatgaatt tgaaaacctg gttcgtaatg
    19321 tcgacgtaaa atcgcgaatt atggatcagt atgctgactg gaaaggcgta cgctatcgtc
    19381 tgggcggcag cactaaaaaa ggtatcgatt gttctggttt cgtacagcgt acattccgtg
    19441 agcaatttgg cttagaactt ccgcgttcga cttatgaaca gcaggaaatg ggtaaatctg
    19501 tttcccgcag taatttgcgt acgggtgatt tagttctgtt ccgtgccggt tcaacgggac
    19561 gccatgtcgg tatttatatc ggcaacaacc agtttgtcca tgcttccacc agcagtggtg
    19621 ttattatttc cagcatgaat gaaccatact ggaagaagcg ttacaacgaa gcacgccggg
    19681 ttctcagccg cagctaataa accgtttgga tgcaatccct tggctatcct gacgagttaa
    19741 ctgaaagcac tgcttaggca gtgctttttt gttttcattc atcagagaaa atgatgtttc
    19801 cgcgtcttga tccaggctat agtccggtca ttgttatctt ttaaatgttg tcgtaatttc
    19861 aggaaattaa cggaatcatg tttatacgcg ctcccaattc tggacgtaag ctcctgctta
    19921 cctgcattgt tgcaggcgtg atgattgcga tactggtgag ctgccttcag tttttagtgg
    19981 cctggcataa gcacgaagtc aaatacgaca cactgattac cgacgtacaa aagtatctcg
    20041 atacctattt tgccgacctt aaatccacta ctgaccggct ccagccgctg accttagata
    20101 cctgccagca ggctaacccc gaactgaccg cccgcgcggc gtttagcatg aatgttcgta
    20161 cgtttgtgct ggtaaaagat aaaaaaacat tctgttcatc tgcaactggt gagatggaca
    20221 ttccactcaa tgaattgatt ccggcgctcg acattaataa aaacgtcgat atggcgatct
    20281 tacccggcac gccgatggtg ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt
    20341 tgctgaaaaa tagcggcgtg tttgccgctc tgaatctcaa cctgacgcct tcactctttt
    20401 atagttcacg gcaggaagat tacgatggcc tcgccctcat tattggtaat actgcgctat
    20461 ctaccttttc ttcacgtttg atgaacgtta atgaattaac cgacatgccg gtccgtgaaa
    20521 ctaaaattgc gggcattcct ctgaccgttc ggctttatgc agatgactgg acatggaacg
    20581 atgtgtggta cgcattttta ctgggtggca tgagtggaac tttcgttgga cttctctgct
    20641 attacctgat gagtgtgcgt atgcgcccag gcagagaaat catgaccgcc atcaagcgcg
    20701 aacaatttta cgtggtatat caaccggtgg ttgatacaca agctttgcgg gtaacgggcc
    20761 tggaagtact gctacgctgg cggcatcctg tagcaggaga aatacccccg gatgccttca
    20821 ttaactttgc cgaagcgcaa aagatgattg tgccactgac tcagcacctg tttgagttga
    20881 ttgcccgcga tgccgcagaa ttagaaaaag tactgccggt aggcgtcaaa tttggcatta
    20941 acattgcgcc ggcccacttg cacagcgaaa gctttaaagc ggatatccag aaactgctca
    21001 cttccctgcc cgcacaccat ttccagattg tgctggaaat taccgagcgc gatatgctga
    21061 aagagcgaga agccacacaa ctcttcgcct ggctgcattc ggtcggcgta gaaatcgcta
    21121 ttgatgactt cggcaccggg cacagcgcgc ttatctatct tgagcgtttt acgctcgatt
    21181 atctgaaaat tgatcgtgga tttatcaacg ccatcggtac ggaaacgatc acttcacccg
    21241 tacttgacgc ggtgctgacg ctggcgaaac gtctcaatat gctgacagtt gctgaagggg
    21301 tcgaaacgcc agaacaggca cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct
    21361 actggattag tcgcccgtta ccgctggacg attttgttcg ctggctgaag aaaccgtata
    21421 cgccgcagtg gtaaggtgtg cttacgtccc ttattattca tagtgaaagc atgccggatt
    21481 gcggctaatg atgagtaaaa ggaaatccgt tgcagatgat tgtgcgcata ctgctgctgt
    21541 ttatcgctct gttcaccttt ggtgcgcagg cgcaggctat caaggaaagc tatgcctttg
    21601 cagtactggg cgaaccccgg tacgcattta atttcaacca ttttgattat gtgaaccccg
    21661 ccgcgccaaa aggtgggcaa ataacgttgt ctgccctcgg caccttcgat aatttcaacc
    21721 gctatgcact gcgcggcaat ccgggcgcac gcaccgagca gctgtacgac acgctattta
    21781 cgacttccga tgacgaacca ggcagttatt acccgctgat tgctgaaagc gcacgctatg
    21841 ctgacgatta ttcctgggtg gaggtcgcta ttaatccacg cgcccgtttt catgatggtt
    21901 cgcccattac tgcccgcgat gtagagttta cttttcaaaa atttatgacc gaaggcgtgc
    21961 cgcaatttcg tctggtctac aaaggcacca ccgtcaaagc cattgcgccg ttaaccgtgc
    22021 gcattgagtt agctaaaccc ggcaaagaag atatgctgag tctgttttcg ctgccggtat
    22081 ttccagaaaa gtactggaaa gatcacaaaa ttagcgaccc gctcgccacg cctccgcttg
    22141 ccagtggtcc gtaccgcatt acgtcctgga aaatggggca aaatattgtc tattcccgcg
    22201 tgaaagatta ctgggcagca aacttaccgg taaaccgtgg acgctggaat ttcgacacca
    22261 ttcgctacga ttattacctc gatgataatg tcgcctttga agcgtttaaa gcaggagcct
    22321 ttgatttgcg tatggaaaac gacgccaaaa actgggccac gcgctatacc ggtaaaaatt
    22381 tcgataaaaa atacatcatc aaagatgagc aaaagaacga atcagcccag gatacgcgct
    22441 ggctggcgtt taatatccaa cgtccggtat tcagcgatcg ccgggtgcgg gaagcgatca
    22501 ctctcgcctt tgactttgaa tggatgaaca aggcgttgtt ttacaatacc tggagtcgca
    22561 cgaacagtta ttttcagaat accgaatatg cggccagaaa ttaccccgac gccgcggaac
    22621 tggtgcttct ggcaccaatg aaaaaagatc taccgccaga agtcttcacg caaatctacc
    22681 agccgccggt atccaaaggc gatggctacg atcgtgacaa cctgttaaaa gccgacaaac
    22741 ttctcaacga agcaggctgg gtgctgaagg gtcagcaacg cgttaatgcc acaacaggtc
    22801 agccactcag ctttgaatta ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt
    22861 tccagcacag cctgcaacgg ctgggtatca acatggacat tcgcaaggtg gataactcgc
    22921 aaatcaccaa ccgcatgcgc agtcgcgact atgacatgat gccgcgcgta tggcgggcga
    22981 tgccgtggcc cagttccgat ttacagattt cctggtcatc ggaatatatc aattccactt
    23041 ataatgcccc cggcgtgcaa agcccggtta tcgactcgct gatcaaccaa attattgccg
    23101 cgcagggaaa taaagaaaaa ttactgccgt tgggacgagc actggatcgc gtattaacgt
    23161 ggaattatta catgctgcca atgtggtaca tggcggaaga ccgtctcgcc tggtgggata
    23221 aattctccca acccgctgta cgcccagttt acagcctggg tattgatacc tggtggtatg
    23281 acgttaataa agcgacgaaa ctgccgtcag ccagacaaca gggagagtag atgggcgctt
    23341 atctgatccg ccgtctgttg ctggtgatcc caacgctatg ggcgattatc actatcaact
    23401 ttttcatcgt gcaaattgcg cctggcggtc cggtcgacca ggccatcgcc gccattgagt
    23461 ttggcaatgc cggagtatta cccggcgcag gcggtgaagg tgttcgcgcc agccatgcgc
    23521 aaacgggcgt cggcaatatc agcgacagta attaccgtgg cggacgcgga ttagatccag
    23581 aagtgatcgc cgagatcact catcgctacg gtttcgataa gccgatccac gaacgttact
    23641 tcaaaatgct ctgggactac atccgttttg attttggcga cagccttttt cgcagcgcct
    23701 cggtgctgac gctgattaaa gacagtctgc cggtctccat caccctcgga ttgtggagca
    23761 cgctgattat ctatctggtg tcgattccgt taggcattcg caaagctgtt tataatggga
    23821 gccgttttga cgtctggagt agtgcattta tcatcatcgg ctacgccatt ccggcctttt
    23881 tgtttgccat cctgctgatt gtcttcttcg cgggcggcag ctatttcgat ctgttccctc
    23941 tgcgcggcct ggtttccgct aactttgatt cgctgccgtg gtatcagaaa atcaccgatt
    24001 atctgtggca tatcacgctg ccggtgctgg cgacagtgat tggcggcttt gcggctctga
    24061 ccatgctgac aaaaaactca ttccttgatg aagtgcgtaa gcaatacgtg gtgacggcac
    24121 gtgcgaaagg ggtaagtgaa aaaaatattc tctggaaaca tgtgttccgc aacgccatgc
    24181 tgctggtgat tgccggtttt ccggcgacgt ttatcagcat gttttttacc ggctcgctgc
    24241 tgattgaggt gatgttttca ctcaatggtc ttggcttact gggctacgaa gcgaccgtct
    24301 cgcgcgatta tcctgtaatg tttggtacct tgtatatttt caccctgatt ggcctgctgc
    24361 tgaatattgt cagtgatatc agctatacgc tggtcgatcc gcgtatagac tttgagggac
    24421 gttaatgtcg cgactcagcc ccgtcaatca ggcccgttgg gcgcgttttc gccataaccg
    24481 tcgcggctac tggtcgttat ggattttcct cctcttgttt ggtttgagtt tgtgttctga
    24541 acttatcgcc aacgataaac cgttgctggt gcgttatgac ggcagttggt atttcccgtt
    24601 gttgaaaaac tacagcgaaa gcgattttgg cggcccgctg gcaagtcagg ctgattatca
    24661 ggacccgtgg ctgaaacaac gactggaaaa taacggctgg gtactgtggg caccgattcg
    24721 ctttggtgct accagtatca actttgctac cgataagccc ttcccttctc caccctcccg
    24781 gcaaaactgg ctgggaacgg atgccaatgg cggcgatgtg ctggcgcgca ttctctatgg
    24841 cacgcggatc tcagttctgt ttggcctgat gctgaccctc tgctccagcg tgatgggtgt
    24901 gctggcgggg gcgctacaag gctattacgg tggtaaagtc gatctctggg ggcaacgttt
    24961 tattgaagta tggtcgggga tgccgacgct gtttttgatt attttacttt ccagcgtcgt
    25021 acagcctaac ttctggtggc tgctggcaat tactgtcttg tttggctgga tgagtctggt
    25081 cggcgtggtg cgggcggagt ttttacgtac ccgtaatttc gactacatcc gcgcggcgca
    25141 ggcgcttggc gtcagcgatc gcagtatcat cctgcgtcat atgttgccaa atgccatggt
    25201 cgctaccctc acttttttac cgtttatttt atgtagttcg ataaccaccc tgacctcact
    25261 cgatttcctc ggcttcggtc tgccgctcgg ttcaccgtca ctcggcgaac tcctgttaca
    25321 agggaaaaat aaccttcagg ccccgtggct tgggatcacc gccttcttat cggtggcgat
    25381 attgttgtct ttgctgatct ttattggtga agccgtccgc gacgcatttg atcctaataa
    25441 ggcggtgtag catgacgcaa actctgttag cgattgaaaa tttgtcggtg ggttttcgcc
    25501 atcagcaaac tgttcgtacc gtagtgaatg atgtgtcact acacattgag gctggcgaaa
    25561 cgctggcgct ggtaggtgag tctggttcag gtaagagcgt taccgcgctg tcaattttac
    25621 gcctgctccc ttgcccgccg gttgaatatc tctccggcga tattcgtttt catggcgaat
    25681 cgctgcttca tgccagcgat caaacgttgc gcggtgtacg cggtaataag atcgccatga
    25741 tttttcagga gccgatggtg tcgttaaatc cattacatac cctggaaaaa cagctttatg
    25801 aagtgctttc actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa attcttaact
    25861 gccttgatcg cgtcggtatc cgccaggcgg caaaacggct gacagattat ccgcatcagc
    25921 tctccggcgg cgaacgccag cgggtgatga ttgcgatggc gctgttaacg cgaccggaat
    25981 tattaattgc cgatgaacca accaccgcgc tggacgtctc tgtccaggcg cagattttac
    26041 agctgttgcg cgaactgcaa ggcgagctga atatgggtat gctgtttatt actcataacc
    26101 tcagcattgt tagaaaactg gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg
    26161 agaaaaataa cgccgctacg ctatttgcct cacccactca tccttacaca caaaagctac
    26221 tcaacagtga accatctggc gacccggtgc cattgccaga acctgcctca acgttgctgg
    26281 atgttgaaca acttcaggtt gccttcccca ttcgcaaagg gattttgaag cgcattgtgg
    26341 atcataatgt ggtggtgaaa aacatcagtt ttacgctacg ggcgggtgaa acactgggtt
    26401 tagtgggcga gtccggttcc gggaaaagca cgacgggact ggcgctgctg cgactgatta
    26461 actctcatgg cagcatcgtc tttgacggtc agccactgca aaatttaaat cgccgccagc
    26521 tgttacctat tcgtcatcgc attcaggtgg tatttcagga tccaaactcc tcactcaacc
    26581 cacgactcaa cgttttgcaa attattgagg aaggcttacg ggttcaccag ccgacgcttt
    26641 ctgccgcaca acgcgaacaa caagtgatag ccgtgatgca tgaagtggga ttagatcctg
    26701 aaacacgcca ccgttatccg gcggagttct ctggtggtca gcggcaacgt attgcgattg
    26761 ccagggcgtt aattcttaag ccctcgctga tcattcttga tgaaccaaca tcatcactcg
    26821 acaaaacggt gcaggcgcaa atattgacgc tattgaaatc attgcaacaa aagcatcaac
    26881 tggcctattt gtttatcagc cacgatttgc acgttgtccg cgcgttatgt catcaggtta
    26941 tcgtactgcg acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg tttgccgcac
    27001 cgcagcagga gtatacgcgt cagctactgg cgttgagctg acgcttaaaa aggattgtag
    27061 tttgaaaagg gttcggcaat tgccacacca aaatttttca atcggcatgt tgcagcaaat
    27121 tcgtcctggc gatttacaaa caggcacggc tcaccttcac actccagcac cgaacatgga
    27181 acttcgatgt cgcttaacgc ctggcttagt ttgtacatca ccgaccatgc attatcacca
    27241 tccgggctaa gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc attttgcgga
    27301 atcggttcaa ccttcgaacc tgcgaaacca gcagaccagc gatagttctg cggcagtcga
    27361 tggacgattg agagttgtaa tgaagtcact tattttcccc ggaagtacat tttacttcac
    27421 aatttattta catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag
    27481 attgatgcct cgcctgcgtg gcctatatgg ctctgattta aataatatct gcgggcgtgg
    27541 tcacgtttgc ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca
    27601 atgatgtgac tttttacata aattgatttt acataaaata aacatatatc gggggaatga
    27661 tagatttgtg gttgacatgc atcaacaaag gaagcctttt agcttcctcg ttgtgcaata
    27721 gatcaccgtt ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcacag
    27781 aatgcaattg accaaatcat cggccatgca gagttaaagg tcgcaagaga aagtaatgcg
    27841 ccaacaattg cccctatgcc gaaacggaag gttcctgcca gcgaagatgc cgttcccgcc
    27901 atatggggaa actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag
    27961 cccacaaacg ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagtgcactg
    28021 atgaccatcc acgctgccat aataaactgt atccacaacc ccgagcggaa catatttaac
    28081 gcgccaatac ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaagaatg
    28141 tttagcgcaa agtaataacc aaagttttcc ggcgcgacgt ggttgatttc aatgtagaca
    28201 aacggtccgg cgcttaagaa tgagaacatc ccggcaaagc tgaaaccgct ggccagcata
    28261 taactcagga cacgcttatg acggaacagc gccgcaaagt taccaatagt ggtacgaatg
    28321 tgaaaaggct ggcgacgctc cggtggtaag gtttctttga tcagaaagaa gatcattgcc
    28381 gaagccagaa tcgccgccac ggcgaggatc cagaagatgt aatgccagct cagccacacc
    28441 agcacccagc cgccaactat cggtgccatc agcggtgcaa tggtcgtcac cagcatgaca
    28501 aacgacatca tccgtgagaa ctcttctttc gggtagatat cacgcatcaa agcgttgata
    28561 accacgctgg ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg
    28621 atggtttgtg ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt accgccaaga
    28681 accaccggct tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca
    28741 aagcccagaa tataagtact gagggtcatc tgcgtactgc ccgccggtac accaaactgc
    28801 gctgaaatta ccggtagcgc aggcagatac atatcaatcg acagcggcat caacatggcc
    28861 agcaggccaa ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc
    28921 tcctgaaagt cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga
    28981 tattcaccgg gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc
    29041 tcaaccacat ggttacccac ggcggcgaac atacgtttca cctgatgata acgcccttcg
    29101 ctgatggtca aacgaacctg cgttggggta atcacttcca gcaccgccgg cttagtgaga
    29161 tctttttcgt tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctacg
    29221 ggtgattcca gcgtcaccag ataggtcttc tcgcaatgat ggcgcgggga agtaatgcgg
    29281 tgcgaccact gaccatcatc tgtcattagt accagaccgg tggtatcgat atccagacgc
    29341 cctgcagcgt gcagtttcca cgctaccggt tcatcaagaa aatagagcac cgttgggtga
    29401 tccgggtcgt ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga
    29461 ccgtgttgct gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg
    29521 aacgctgcat tacggacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc
    29581 ccggcaatag cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt
    29641 gtgattttgc ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct
    29701 gcccagtata gcggtctaat aacttccctc aagggaaaaa gatccgtggc atactattag
    29761 cagaataatc tacctacgcg agaccatgat ttttacactc cgcccatatc agcaagaagc
    29821 cgtggatgcc acgctcaacc attttcgtcg tcataaaacc cctgccgtaa tcgtgctgcc
    29881 caccggtgca ggtaaaagcc tggtgatagc ggaactggcg cggctggcgc gtggtcgcgt
    29941 gctggtgctg gcacacgtta aagaactggt ggcacaaaac catgcaaaat atcaggcgct
    30001 ggggctggaa gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt
    30061 ggtatttggc agcgtgcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc
    30121 gctgttgatt gtcgatgaat gccaccgtat tggtgacgat gaagagagcc aatatcagca
    30181 aatcctcact cacctgacca aagtgaatcc ccacttacgc ctgctggggc tgactgccac
    30241 gccttttcga ctgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg
    30301 cgatgagaaa gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa
    30361 acacggctat ctgacgccgc cagaacggct ggatatgcca gtagtgcaat acgatttcag
    30421 ccgcttgcag gcacagagta acggactgtt cagcgaagcc gatctcaacc gcgagctgaa
    30481 aaaacagcaa cgcattacgc cacacatcat cagccaaatc atggagtttg ctgcaacgcg
    30541 caaaggggtg atgattttcg ccgcgacggt cgaacacgca aaagagatag tgggattact
    30601 gcctgccgaa gatgcagcac tgattactgg cgacacaccc ggctctgagc gcgatgtgtt
    30661 aattgatgat tttaaagccc agcgttttcg ctatctggtc aacgtcgcgg tactgaccac
    30721 cggatttgac gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcggtgag
    30781 tctttaccaa caaattgtcg ggcgcggtct gcgcctggcc cccggcaaga ctgattgctt
    30841 aattcttgat tatgcgggta accctcacga tctctacgcg ccggaagttg gtacaccgaa
    30901 aggcaaaagt gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac
    30961 cttttggggg aaaacgaccg ccgacgggac attgattgaa cactttggtc gtcgctgtca
    31021 gggatggttt gaagatgacg acggacatcg cgaacagtgt gacttccgtt tccgttttaa
    31081 aaattgcccg caatgtaacg ctgaaaatga tattgccgcc cgccgttgcc gcgagtgtga
    31141 caccattctg gttgacccgg atgatatgtt aaaagcggcg ctacgactga aagacgcgct
    31201 ggtattacgc tgtagcggca tgtctttgca gcatgggcac gacgaaaaag gcgaatggtt
    31261 gaaaatcacc tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac
    31321 gcccgcccag cgaactgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg
    31381 catcccgctg cgctggatca ccgccgccga tgtcctcgcc cagcaagcct tattgcgaca
    31441 cccggatttt gtcgtcgccc gcatgaaagg ccagtactgg caagtgcgtg aaaaagtgtt
    31501 cgattacgaa ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt
    31561 gatgtataag gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac
    31621 ttacctgttg ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta
    31681 tcaacgcaga agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta
    31741 acaagttccc ggcaatcatc tacggtggca aagaagcgcc gctggctgtc gaactggatc
    31801 acgacaaagt catgaacatg caagttaaag ctgaattcta cagcgaagtt ctgaccatcg
    31861 ttgttgacgg taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac
    31921 cgaagctgct gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc
    31981 cgctccggcg gggttttttg tctctgcaaa ttatgcctga ttacggtatt gctatttttt
    32041 gcaggccaga taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg
    32101 ccgccagacg tccggcgctg caattgatcg cgcaagttcg gcggcgtgcc tttaatggtc
    32161 agggtatcgg ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta
    32221 atagtcaagc caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct
    32281 gccgggaagc tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact
    32341 tcgctgacgc ctgccagctc tttcgacagc gattccagtt caatctcttc acctgcctgt
    32401 agctgctcat tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc
    32461 agttgtgctt cggcagtgaa atcatcgacc gcttgcaaca gcccacggtt ttgcgctttc
    32521 gcgtttaacc cttcgctggc accgaggaaa tccataaaga aatccgccac tttgcgccct
    32581 actcgccctt tcaggaaagt gagatagcgg gtcgactctg gattggtttc ccactcggtt
    32641 aaatcgatac gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg
    32701 tcgagatttt cgttaacacg catactgctc aggttgctca gaactgccac cagcagatac
    32761 tccaccgcca gatagcgata gtggcaaaac agcacgaatc caccgtcagc aaaaggatat
    32821 ttcgctaatt cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct
    32881 tcgccctgac gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg
    32941 taagctttat ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt
    33001 tccgtcggtt ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg
    33061 ataagctggt gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga
    33121 ccgggcggta ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac
    33181 agaaggcggc gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta
    33241 cggtaatatg ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc
    33301 tacagtgatg aacaggttga acaattgctc gcagagctgc tcaacatact ggaaaaacat
    33361 aaggctccga ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac
    33421 accagcattg ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag
    33481 tcctctatca acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca
    33541 tcgtcagcgc taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact
    33601 gttcaatatt ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc
    33661 gacaacgctt gctggtcgca tttattccta cgtaagcatt atcggtcatt tcagcttcct
    33721 ggtgttcgcc acctacttgc tgatcctctt cccgctaacc tttatcgtcg gctcccagag
    33781 gctgatgagg tttttgtccg tcattctggc aacggcggga atgacgctat tactgatcga
    33841 cagcgaagtc tttactcgtt tccatctcca tcttaatccc atcgtctggc agctggttat
    33901 caacccagac gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt
    33961 tattttattg cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac
    34021 gcgtcgtcgg cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc
    34081 gcatgtggtt tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc
    34141 taacctgccg ctttcgtacc cgatgacggc acgacgtttt cttgagaagc atggcctgct
    34201 tgatgcgcaa gagtatcaac gccgtcttat tgagcaaggc aatccagacg ccgtttccgt
    34261 tcagtatccg ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt
    34321 gattactgtc gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg
    34381 ttttgctgag caaaatattt cgttcacgcg tcatatgagc tccggcaaca ctacagacaa
    34441 cggcatcttt ggcctgtttt atggcatctc gccaagctat atggacggca ttttgtcgac
    34501 ccgtacgcct gcggcgttaa ttactgcgct taatcagcaa ggctatcagc tggggttatt
    34561 ctcgtcagat ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat
    34621 gccgagtgta cgcacccaat ccgacgaaca gaccgccacg cagtggatta actggctggg
    34681 ccgctacgca caagaagata accgctggtt ctcgtgggtc tctttcaatg gcacaaacat
    34741 tgacgacagc aatcagcagg catttgcacg gaaatatagc cgggcagcag gcaatgtcga
    34801 tgaccagatc aaccgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt
    34861 ggtgattatc actgccgggc ggggtattcc gctgagcgaa gaggaagaaa cctttgactg
    34921 gtcccacggt catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg
    34981 tattaatgcg ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctaca
    35041 tgtcagcaca cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg
    35101 ccattactgg gttaccgccg cggataacga tacgctggca attaccaccc cgaaaaagac
    35161 gctggtgctg aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa
    35221 agatgaaaaa ccacagttaa gtttgttatt gcaagtactg acagacgaga agcgttttat
    35281 cgctaactga ttaattataa atcagttagc aaaatatctt acttgcaatc ggtgtggaaa
    35341 acggtagtat tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg
    35401 ggtgtcgggg gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaccaa
    35461 cctattgcgg ttggtttttt tatatctgca tttaattcga taaacagacc gcgacacatc
    35521 acggcctgtt tattttctgt tatcagaacg tccagaccac acccgcctga gttgtgctgc
    35581 tttcttcacg ggagaaacgg ctgtcgatac caagcgttag ccccgtcaat gtttgctcaa
    35641 agccagcgcc tgcatcagtg gtcacgttgt tgcggctgtt aattgccgaa ctccacatcg
    35701 ccgtatcgta attaacgcct tttacgctac caagacgccc acgcacggtg tccagttctg
    35761 catcaaatac cagcggttgt gctgccgcca tattcagcac atccgtggtt gaagggggca
    35821 tttgtgcgcg attctctgcg agactccagc tatggttgcc attatccagc aaggtatatt
    35881 catacgtact gatatcaaca acgcctccgg cattgcccaa cgtaaatgcc gcatcaccgc
    35941 gcccgttgtt accagtgtaa ggttatctcc tgctgccggg ctggcaccgg tgtccgtcac
    36001 gaatattttg aaatcacctg ttgctttaac ctccagtagc cctccctttt ctataacggc
    36061 aaagacggca atcccatctt tttcaacaat taagcgccct cctttaatcg tgggatcagt
    36121 cgccgcgcca taaacctgta gcactccatt attgatcgta gtctggttta ccgcgccacc
    36181 catttgaaca atttgataac catcatttaa tactgagtat tctgcggtgc catttatttc
    36241 aatgtactga tatcccccgt taatttcagt attactactt acaccaaatt cttatatatg
    36301 ctgttctccg ccaggattga ttttcgtatt attggcggcc ctacaaacca gctggatatc
    36361 tttttccagg acaacaccat caactgtctc accatactca acagttgatg ccaatgctat
    36421 gggcgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa gagaaacaga
    36481 accagattgg tgcattaagc tttcatccta tgaaattaat tgctgttaaa agcattgggg
    36541 acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt taaagaaatg
    36601 cacaagtatt gtgattgatt ttttagttgt ttttcctgat gagaagctga tgcaaaattc
    36661 cgtctttata ataaagatga tgccaaagcg aacgacaaag ttgtagtttt cactacatgt
    36721 ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc tacgctcaaa
    36781 cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc cttttcaggt
    36841 gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac tggagcttga
    36901 ttctggcttt gaagtggtcg ccgaagcggg cgacggcgca agcgcgatcg atctggcgaa
    36961 tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggcatga gtggcctgga
    37021 taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc tgaccgtatc
    37081 agatgcctcc agcgatgtct ttgcactgat agacgcaggc gcagacggtt atctgttgaa
    37141 agacagcgac ccggaagtgt tactggaagc gattcgtgcc ggagcgaaag gcagcaaagt
    37201 ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg cggaagaaga
    37261 ccccttcagc gtgctgacag aacgcgagct ggatgttctg cacgagctgg cacaggggct
    37321 gtcaaataaa cagattgcct cggtgttgaa tatttccgag cagacagtaa aagtccatat
    37381 tcgcaatctg ctgcgtaaac tcaacgtccg ctcacgcgtg gcggccacca ttctgttcct
    37441 gcaacaacgc ggggcacaat aaaaatagcc cgatggcatc accatcgggc cactttttat
    37501 ttactctcct gcggcgacaa atgttgcatc gcctgcgcga tactacgttc gatcaccgca
    37561 cggcgagtat cgttggcagg taagagtttc aacatcattt cccacgcggc aacggcttcg
    37621 ccaaatcgtt gctgctcaaa ggcattaaat gcatacatgc ttaacacacg gatattgcta
    37681 tggtccgttc tcaccaactg gcgcagcagt tcgccgccga ggcggttgtc gttgggatca
    37741 gatgaacgtg tcaacgcttc agcgtatccc agtgcagcat cgctgttttt cggatcgagg
    37801 cgatacgcag tggcgtatgc atcggtggcg atactggcgt tacccagcgc catgccaacg
    37861 cggcccaaca taatccagcc ttctatatct cccggatttt tttgcagttg agtacgcatc
    37921 cccagcgcaa ggcgcgacat ctcttcttcg ttgagcggat cggctttcgg atccagcgcc
    37981 ctgtccagta acgccggagc ctgtgccgtg gcctgctgcc agattttcac ctgctgataa
    38041 ttgccagtct ggtagtagct gacgccagcc acaattaacg ccaccacaat acccggcaga
    38101 taaacaacat atccggcacg cttaccttcc ggcacgcttt gttctggaaa cgcttccggc
    38161 accacgcgta cccgacgccg cgaacgggcg tatatgaccc aaccgccaat gccaatagcc
    38221 actactggca gcacccacag cagcacggtc agcggcgtta acggcggatc gtaagtgacg
    38281 aagttgccgt aacgcgccac catataatcg acaatctctt tcttactttt gccttcctgc
    38341 atcagttcat acactttctg gcgcaggtcg gtggcaatca tcgagttgga atcggcaatg
    38401 ctgttgttct ggcatttcgg gcagcgcagt tcttcagtga gctgacggaa ctgttgttcc
    38461 tgtgcttcat ctttaaactg caacacgtcg atggtcgcca gcgctgagcc ggagatcatc
    38521 agcatcagca cgcccaataa aaacctcatt gtgcggcctc cttactgtat ttctcccaca
    38581 gtggcttgat ctcttcttcc cagacgcgag gattgagatc gcccgcatgg cgatagcgaa
    38641 tgatgccgtt gccgtcaata aggaacgttt ctggcgcgcc atagacaccg agatccagcc
    38701 ctaacatgcc gtcgccatca aacaggctta gcgcgtaagg attgcccagc tctttcagcc
    38761 agctgattgc cttctggcga tcgtctttat agttcatgcc gaccacgcgg atgccctgcg
    38821 cagaaagctg attcagatat tgatgttccg cacggcaggt cggacaccag gtcgcccaga
    38881 cgttaagcag cactggtttg ccctgagtca gcacatccgc ctgataaaac tgccccggat
    38941 tgtccagtga ttcgaggcga aacttcggca ctggcttgcc aatgagcgcc gattccagat
    39001 tggtcggatc atccccttcg gcattacgcg ccagctgcca cagcagcgcc gcggcaatcg
    39061 ccaggaagat aatcaacgga attaacaata ccttgcgctt catgcagcct ccggcgcagt
    39121 tttctgcggg ttcacgcgct tacgatagcg aggatcaaac agacacagca gtccgcccaa
    39181 cgccatcatc agcccgcccg cccaaatcca gcgaacaaat ggtttgtagt aaagacgcac
    39241 ggcccacgcg ccgttttcca gctcttcacc gagcgccgcg tacaggtcac gcgtgatgcc
    39301 accgtcaatc gccgcttcgg tcatcatcga cccggcagtg ttgtaataac gtttttctgc
    39361 atacagcact gtttccggct tgccatctcg cgttacgccg atagtcgcca caccgccacg
    39421 ccagtttgga ccagtcactt ctttgacatc acggaaggtg aagcgatatt catgaatatc
    39481 gacgctatcg ccggacttca tgcgcacatc acgctcaacg ctatagttct gactaaaagc
    39541 aatgccaaca attgtcactg ccagccccag gtgagctgcc accatccccc aataactgaa
    39601 ggtggttttc gtgccgcgtg aaatacgcag cgcagcttcc gcaattgcca gcaccgcaat
    39661 ccagcaggcc attgccaggc cgagcaccgt catcgccaca actttgcttt cgaacagcca
    39721 cggcaacagc agcgacagca ccagcgtaga gatgaaggcg ataatcaaca aattgcggat
    39781 tttacgtggg cgatcgcgtc cccagcgcac cagagggccg acaccaagca gtagcgcaaa
    39841 cggcaccatc agccaggtaa acatggtgtt gaagaacggt tcgccaatcg aaatactgcc
    39901 cagtcccagt tgcttgtgca ccagcggcag caacgtcccc agcaacacca ccagcatcgc
    39961 ggctaccagc aaaacgttgt tcgctaacag caaagattcc cgcgaccaca gcgcattgtt
    40021 tacgcgtgag cgaactttgt gtccacgcgc ggcaaacagc agcagcgaac cgccaatcac
    40081 cagcaccata aaggcgagga taaacatacc gcgcgccgga tcagacgcaa acgcgtgtac
    40141 cgataccagc acgccggaac gcacgaggaa agtccccagc agacacaacg agaaggcact
    40201 gattgccagc agtaatgtcc acgctttgaa gctggcgcgt tgttcagtga ccgccagtga
    40261 gtgcatcagc gcagtcccca ccagccacgg cataaacgag gcgttttcta ccggatccca
    40321 gaaccaccag ccgccccagc cgagttcgta ataggcccat gcggaaccga gcacaatgcc
    40381 gagcgtcagg aagatccacg cagccagcgt ccacggacga gtaaaacgcg cataagtgct
    40441 gtccagacgc ccactcaaca aagatgcaat ggcaaaagca aacgccaccg agaaaccgac
    40501 gtaccccata tagagcagag gcgggtggaa aatcagcccc ggatcctgca acaacgggtt
    40561 aagatcgcgc ccttcaatcg ggaaattcgg cagcgtgcgt gagaacgggt tagaggtaaa
    40621 gagaatgaac agcaaaaagc cgacactgac catccccatt atcgccagta cgcgggcaac
    40681 aatatccagc ggaatacgct gactaaaaat cgctaccgca aaggtccagc cgctcatcag
    40741 tagcacccac agcaggagcg agccttcatg cgcgccccag gtagccgcca cgcgatacca
    40801 caccggaagc tgggtattgg agttgctggc aacataggtg acggtaaagt cgttgaccac
    40861 gaaagcattg accagcacca gaaatgcgcc agccacagac ataaacagca gccaggcaaa
    40921 caagcgggaa gacgccatca tgcgcgcgtc cccgcgcgcc acgccccata gcggatacac
    40981 agacagcagc agcgcaattc ctagcgccag acacagcagc ccgttaccaa tttctggcat
    41041 catgatgctg ggtccttata aacactcgcc gggcgacggt ggttagcttc catcgctttc
    41101 tcaacttctg gcggcgtgta gttttcatcg tgtttcgcca gcacttcttt cgcgaggata
    41161 tgattgcctt tttccagttc gccctgcacc acaacgccct gcccttcacg gaacagatcc
    41221 ggcaaaatgc cttcgtaaga gacatctact gaaccttcag cgtcgtaaat ggtgaaagtt
    41281 acttttagcg aattgggatc gcgctgcaca ctccccggca tcaccatccc gccaacgcgc
    41341 agacgctgac cgacttccgg catctgctga gtttcacgct tgccgtagag aatctccccc
    41401 ggcgtataaa agagatcgat attcgagcgc agcgcatata gcaccagacc gatagtcagc
    41461 gccagccccg ccagcacggc acaggcaatc cacaagcggt ttttacggcg aatattcatg
    41521 cagcctcctg ctgttgcgca gcacgtaaac gcgcctcacg cgcccgctgt tgcgccacgc
    41581 cacgcagaat tgcgcgatgt tgcatcaccg agtgcacgac taaaaccacc agcggaataa
    41641 cggtcatcac caccgccagc cagacaaaaa aggcgtaacc gcccattgcg aaaaattcat
    41701 tccaggaagc aaatgcaggg gtcatttacg gcctcttttc agtatcagtt cactcaccca
    41761 cggacggcgt ttttccatca gcaaaatcaa attacgcatc cgcatcagcg tcagcgtggc
    41821 agacaggagc aggaagccca aaatcgacca gcgcagcggc gaacgcatcg ccggatcgat
    41881 actttgctgc attcgcgttg atccctgatg cagggtgttc caccactcta cggagtaatg
    41941 aataatcggc agattcacca cgccaatcag caccaggata cctgccgcac gcccagccag
    42001 acgacggtcg tcgaaggcgt gccacagggc aatgacacca acatacagaa acagcagcac
    42061 cagttcagaa gtcagacgtg catcccatac ccaccaggtg ccccacatcg gttttcccca
    42121 tgcagagccg gtaaccaggg caataaaggt aaacacggca ccaatggggg ccatcgccgc
    42181 caccgccagg ttggccattt tcatctgcca gacaaggcca ataaacgccg ccaccgccat
    42241 tgatgcataa atgcccatcg accagatcgc cgcaggcaca tgcaggtaga taatgcggta
    42301 gctatttccc tgctgataat cagccggagc aaagccgaac ccccagatcc agccgacggt
    42361 aagcacgacc acactggcaa ttgccagcca cggtataaac cagccacaga tttgatacag
    42421 ccgtggtggg atcgccagtt gatgcagtgt tttccacata gtttcgatac cagactcgaa
    42481 caaaaatcag taatccagcg ttattgaatg ctgattcgta acgctgccgc cgtcgcaaaa
    42541 ggacttaatg tcgcggtgcc tgccagcaac gcgcctaaaa ttgccagata cccatcaacg
    42601 ggcaaatgca tagaagccgc gtccatcgcg gcggtggcaa agatgagtaa tgggatagtc
    42661 agcggtaaca ccagtatgct gagcagcaca ccaccgcgct taagtcccac tgtcagcgcc
    42721 acgcccggcg caccaagaaa gccaagcgta ggggttccca gcagtagcgt taacgccatc
    42781 acttgccagc cataaacatc cattcccagt agcattgcta ccaggggcga aaggatgagt
    42841 aacggcagac cggttaccat ccagtgcgcc atcaccttcg ccagcacaac ggcgggcaag
    42901 ggtaacggca acaacatcaa ttgttcaaga ctgccgtcct gcaaatcgtc acggaacagt
    42961 cgctcgagtg ccagcaagga tgaaagcagc gcggcaaccc agataatacc cggcgcaata
    43021 cgcgccagca gttgcggctc cggaccgata ctgagcggaa aaagggtaat gacaatcagg
    43081 aagaaccaca gcgggttggc gatttcggcg ctgtggcgaa acgctacgcg cagctcaaga
    43141 cggaaaatgc gccagaacat catgcggccc tcgtttgcgt cagtgaaatg cggcgaattt
    43201 tactttcagc aacgttgagc ggctggtggg tagtcagaat cacaatcccc ccctgctccg
    43261 tatgctgcgc catacgctgg gtcagacgat cgacgccatt aacgtcaatc gcggtaaaag
    43321 gctcgtcgag gatccataac gtggcacggg tcagccacag acgcgctaaa gcgacgcggc
    43381 gttgttgccc ggcagaaagt tgatttaccg gaatatcttc gaatccggca agcccggcct
    43441 gcgccagggc ttccagacat tgtgcggtat cgccatcgcg atgataaaag tgcagatttt
    43501 ctaacgccgt cagccgggtt ttgatccccg gctgatggcc tatccataac aggttttgat
    43561 ggtagctgtc gcgtacctga tgcaagggct gcccttgcca gagaacctcg cctgcatcag
    43621 ggcgagacaa ccccgtcagc aaacggagaa gcgttgtctt ccccgcgccg ttgctaccgg
    43681 tgatttgtac ccactctcct gcgttcagcg taaatgataa accactaaat aaggttcgtt
    43741 catcccgctc gcaaagtaac tctctggctt caagcatact cacgcaataa ccctgttaaa
    43801 aacctggctc gacttcacgc atgtcgggca gcttgtgcgc tatcccttta tggcaatcaa
    43861 tacaggtttg cccatctttc accgcctggt catgcatctt cgcggcaacc gatttctggg
    43921 cggttgtatc catatactcg aagttgtgac agttacggca ctcctgcgag ttgttgtcct
    43981 tcatgcgccg ccactcattc tgtgccatcg tcaggcgatg agcttcaaat ttctgcggcg
    44041 tgtcaataac gccaaaaatt ttaccataca gctctttact tgctttgagc ttgcgtatca
    44101 tctttggcac aaactcgtgc ggaacgtgac aatccggaca ggtcgcacgg acgccgctac
    44161 ggttgttgta gtgcacggaa tccatgtatt cctgatacac cgtgttacgc atttcgtggc
    44221 agctaatgca gaactcttcg gtattggctt tttccatccc ggtgttaaag ccgccccaga
    44281 agacgatgcc accgacaaaa ccgatcaaca gcagcgtccc cagcgccaga cggctggggg
    44341 tacgccacca tttccacagg cgcttaatca gaccaggctt acggtcagaa tttcccataa
    44401 taacctctta tttcccgtaa ccttttgacg gggtaaaggt attccccacg attggcgcgg
    44461 tatcggcctg cggtacgtga cattgcagac agaaataacg acgcggagcc acttccgcgc
    44521 ccactttgcc gtcgctgtcc ataaagtgag taggactaat gcgcggcgcg ccagtggtgc
    44581 gataactttc gacaccgtgg cactgcaagc agcggttggt attggtcgtt acctgataac
    44641 cttcaacgct atgcgggatc attggcggct gattcacata gttcagcggc atccgatcct
    44701 gctcttttgg catccgaatg gccccttcct gcgtcccgga tacttccggc gactggctaa
    44761 aatcgacgcc attagccgcc caaaccgcgc cgcttaccac cagggccagc atcgccgtcc
    44821 attgacacag cgctttcttc aggtcatggc ttttcatgat ttcgctcccg aactccatcg
    44881 tgtagttatt gtaaatacat cctcagaaca gacatccacg cagcgaccgc acgtcatgca
    44941 atcgcggctg gtgacctgca ccgggctttg ttcatccagc accggggcac gtagcacatg
    45001 cggttccggg caaacatgaa aacaatccat acagcggtta catttctgcc gatcggtggc
    45061 cgcaacggta atcacacctt tgctacccag cacgccatac agtgcaccaa ccggacaaat
    45121 gtgcccgcac cagccgtgtt caacgaccag tagatcaaat aaaaacagcg cgagaataag
    45181 cagcgcgccg ctgccgaagc ccattaccag gctacgcccc atcagggaaa cggggttgat
    45241 ccattcccag atgagcgtgc cagttaaggc cgatcccacc agaatgatca ccaacagcac
    45301 gtagcgaatg tggcgaggga tcgtcgcaga ctgattgagg tcaaatcgtc tgcgtaacca
    45361 atttgctaag tcggtaatcg ggttcagcgg gcagacccag ctgcaaaata atcgctttcc
    45421 tgccagggcg tagagcacgg tgataatcac cgcaccagtc agcgctaccg tggcaggcag
    45481 atgaccgctg gcaagacttt gcagtgtcat cagcggatcg gtcagtggaa cggtgtcgaa
    45541 taacaggcta ctgctgtagt tgccgtgcaa gatccacaca ccaaaccacg gaccgctcaa
    45601 aaacatcccc agcacgaaga actgacaaag gcgacgtaac actaaccaac ggtgactgcg
    45661 ccaccagcct tttttctcca gcgcctcgcg cccggcgtca cgtttacgat ttgccattgt
    45721 tcccctccag ccagccgaag cggtaatggt gacctaactc ccctttcgcc agtgacagcg
    45781 gtaacacctt gattgccggt tgttccagca cgcacacttt ttcgcactta ccgcagccgg
    45841 tacaggcgtc gctgtgaacc gtcggcagaa agcgggcgtg cttaccggtt cgcgtgttgc
    45901 gctccagctc cagggtgatg gcctcatcaa ttttcgggca ttcgcgataa caaacatcgc
    45961 agcgcagccc ctgaaagttg agacagtttt cctggtccac cagtaccgcc agccccatcc
    46021 gcgcgtcgtc gatcgattcg atctcacgat ccagcgcacc gctcgggcac actttggcgc
    46081 acggaatgtc ctcacacatt tcgcaaggaa tatcccgtgc gacaaaatat ggcgtgcccg
    46141 ccgacagacc agaggccagc gtcgccagtt ttaaggtgtc gtaagggcaa gcctgaacac
    46201 actgaccgca acgcacacag gcactggcaa aggcgttctc gtttatggct ccgggcgggc
    46261 gcaaccgcac accagatgcg cgtgcggttt gctgttgtaa ccccagcgcc acacccacgg
    46321 cagccagccc gcctgctgtg cgaacgacat cgcgcagaaa gcggcggcga ccattttgag
    46381 gtttcgctga ccgggacata atggcttaca ccttctccag tttgaccgcg cacttcttga
    46441 agtccgtctc tttcgagagc ggatcggtcg catccagcgt cagtttgtta accagctgtg
    46501 cggcgtcgaa gaacggcatg tacaccaggc cctgcggcgg acggttacga ccgcgcgttt
    46561 cgacaatcga gatcacttcg ccacggcgag aaaccacttt cactttgtcg ccacggcgca
    46621 gatcgcgcgc tttcgcatcc agcgggtgaa taaacagaac cgcttccggg aaggcgcggt
    46681 gcagttccgg tacacggcga gtcatactgc cggtatgcca gtgctccagg acgcgtccgg
    46741 tagagagcca caggtcgtac tcttcatccg gcgcttccgc cgcaggttcg aacggcagcg
    46801 cgaagatcac cgctttgcca tccggtttac cgtagaactt gtagccttcg cccgctttca
    46861 cgtacgggtc gttaccttcg ctgtaacgcc actgcgtttc tttaccgttc accaccggcc
    46921 agcgcagacc gcgtgctttg tggtaatcat cgaacggcgc gagatcgtga ccgtgaccgc
    46981 gaccaaacca tgcgtactct tcgaacaacc ctttttgcag atagaagccc agctcgcgag
    47041 attcgtcgtt cagctgatct tccgccagtt cggataccgg gaatttgctc acttccggag
    47101 tggcatacag aacctcatac agcgttttgc cacgcagttc cggtttctta gccagcagct
    47161 cttccggcca cacttcttca gttttgaagc gacgggagaa ctgaactaac tgccagagat
    47221 ccgattttgc ttcacccggt gcctgtacct gctgacgcca gaactgagta cggcgttcgg
    47281 cattaccgta agccccctct ttctctaccc acattgcggt cggcaggatc aagtccgctg
    47341 ccagcgcact gaccgtcgga tatggatcgg aaacgatgat aaagttgcgc ggatcgcgcc
    47401 agcccggcat acgctcttca ttaatgttcg gtccggcctg catgttattg gtacacatgg
    47461 tccagtaaac attcagtttg ccgtctttca gcgcacggtc ttgtgccacc gcgtgcagac
    47521 cgattttcgc cggaatggtg ccactcggga tattccactt cttctcgcaa atatcacgat
    47581 gtttctcgtt agtcaccacc atgtccgcag gcagacggtg agcaaaggtg cccacttcac
    47641 gcgcagtacc acacgcggaa ggctgcccgg tcagggagaa cggaccgcaa cccggctggg
    47701 aaattttgcc ggtcagcagg tgcaggttgt agaccaggtt gttggcccac acaccacggg
    47761 tatgctggtt gaagcccatc gtccagtagg agatgacttt cttgttcgga tcggcataca
    47821 gctgcgccag ttgttctaac tggtctttcg gcacgccggt catttcggcg gttttttcca
    47881 gcgtatattc agcaacgaag gctttgtaat cttcaaagct catcggttcg gaggcgtcag
    47941 aacccggatt cttcgctgct ttttccagcg gatgggtcgg acgtaaaccg tagccgatgt
    48001 ccgtcgcccc tttgcgcagg ttaacgtgtt tgctgaagaa gtcctgattt atcgcattgt
    48061 tttgaatgat atagttggcg atatagttca ggatcaccag gtcagattgc ggcgtaaaga
    48121 tgatgccgtt atccgccagc tcgaagctac gatgctggta ggtagaaagc accgccacgg
    48181 taacgttctg gttagagaga cgacggttag tgatgcgcga ccagaggatc gggtgcatct
    48241 ccgccatgtt tgcgccccac agcacaaacg cgtcagcctg ctcgatgtcg tcatagcagc
    48301 ccatcggctc atccatacca aaggtacgca taaagccaac tactgccgac gccatacagt
    48361 gacgcgcgtt cgggtcgatg ttgttcgaac ggaagcccgc tttaaacagc ttggacgcgg
    48421 cataaccttc ccagatagtc cactgaccag aaccgaacat accgatcgat tccggccctt
    48481 tttctttcag ggcggttttg aatttctctt ccatcacatc gaaggcctga tcccaggtga
    48541 ttggggtaaa ttcgccttct ttatcatatt taccgttttt catacgcagc agcggctgcg
    48601 tcaaacggtc tttaccgtac atgattttgg gcaggaaata gcccttaatg cagttcaggc
    48661 cacggttaac cggtgcgtcc gggtcgccct gacaagccac cacacgcccc tgctgcgttc
    48721 cgaccagaac gccgcaaccc gtaccgcaga aacggcacgg cgctttatcc catttaatgg
    48781 cttcctgctg accaacaacg gcgcgggcaa cgcccggcac gctgagaccg gcagccgccg
    48841 cagcggccgc aacggcgtta gctttcataa agctacgacg actgagtttc atggtgtttc
    48901 ctcaccttgc tcttcctgct ggtgataaac cagcgacacc gccagcacgc cctctacgtt
    48961 gcgtactgac tcaatggttt ggatcagcgt ttcgctgtct tctgcttcca ccaccacaat
    49021 caactgaccg ctcggcgcgt cgctgacagc aacttcacag ccggtaaagg cgttcagttg
    49081 ggtgctgatg tctgaaattc gttcgctttt ggcctgcacg accaggctgc aaacttgcca
    49141 gttagtgtgc atggagatac tccgcagtta tggctgatac cggacagctg gctgcacatg
    49201 ctccacatcc gttgcaaagt tggctattaa gttgcggctg gtagatcccg gagagcgtcg
    49261 ggcgaaagat aattgccatt ggctcacagc tatcctgaca gcggcggcat tcaactgact
    49321 gatacgccag gcacgcgtcc ccaatggtga actgcaaatc ccaggccctg gtgtggcgcg
    49381 gagaaaatag cgattcgggg caagcctgcg cgcaggcgta acagaagctg cactcgttat
    49441 ttttgaaatt aacgctcgga tagctacccg cgccgcgttg cagaatgttg ttttcacaag
    49501 cattaataca ggcgtcgcag cgggtgcaat gggtcagaaa atgagattca tcaccggacc
    49561 agggcggacg gataccgtta ctggctttgc gccagcgacc agtgagtatg ccccgacggg
    49621 atgcatcaat cttcacattg accttccatc attaacgcgc tctgaaaatt gagagcgacc
    49681 aaataaaccg cataattaat aagccatttt tatagccgct aagatattaa aggatgtgtc
    49741 aaagatgcat accccgatcg gggtaaaacc tgtagcagga tcaaaagagt ggcgggaagc
    49801 gtggcaaaaa cgggcttttg ctcacatttc aaatg
//
LOCUS       DAPQZD010000014        47901 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPQZD010000014 DAPQZD010000000
VERSION     DAPQZD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.014"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            77..997
                     /locus_tag="RO454_000829"
     CDS             77..997
                     /locus_tag="RO454_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0932704.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            1068..1706
                     /locus_tag="RO454_000830"
     CDS             1068..1706
                     /locus_tag="RO454_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0932705.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1997..2725
                     /gene="mpaA"
                     /locus_tag="RO454_000831"
     CDS             1997..2725
                     /gene="mpaA"
                     /locus_tag="RO454_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDY0932706.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2700..3665)
                     /gene="ycjG"
                     /locus_tag="RO454_000832"
     CDS             complement(2700..3665)
                     /gene="ycjG"
                     /locus_tag="RO454_000832"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDY0932707.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3784..4290
                     /gene="tpx"
                     /locus_tag="RO454_000833"
     CDS             3784..4290
                     /gene="tpx"
                     /locus_tag="RO454_000833"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDY0932708.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(4334..5875)
                     /gene="tyrR"
                     /locus_tag="RO454_000834"
     CDS             complement(4334..5875)
                     /gene="tyrR"
                     /locus_tag="RO454_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDY0932709.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6023..7084)
                     /locus_tag="RO454_000835"
     CDS             complement(6023..7084)
                     /locus_tag="RO454_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDY0932710.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7081..8478)
                     /locus_tag="RO454_000836"
     CDS             complement(7081..8478)
                     /locus_tag="RO454_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDY0932711.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8634..9632
                     /locus_tag="RO454_000837"
     CDS             8634..9632
                     /locus_tag="RO454_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDY0932712.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(9683..10588)
                     /gene="ompG"
                     /locus_tag="RO454_000838"
     CDS             complement(9683..10588)
                     /gene="ompG"
                     /locus_tag="RO454_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDY0932713.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNNWHFNIGAMYEIENVEGYV
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSLGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10633..11715)
                     /locus_tag="RO454_000839"
     CDS             complement(10633..11715)
                     /locus_tag="RO454_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0932714.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     IQKIAKEMIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(11729..12388)
                     /gene="pgmB"
                     /locus_tag="RO454_000840"
     CDS             complement(11729..12388)
                     /gene="pgmB"
                     /locus_tag="RO454_000840"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDY0932715.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(12385..14652)
                     /gene="ycjT"
                     /locus_tag="RO454_000841"
     CDS             complement(12385..14652)
                     /gene="ycjT"
                     /locus_tag="RO454_000841"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDY0932716.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGRDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNERLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(14649..15704)
                     /locus_tag="RO454_000842"
     CDS             complement(14649..15704)
                     /locus_tag="RO454_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDY0932717.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASLWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFAFDTQQLREQVTNGVLGEIYVTTARA
                     LRRSGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(15714..16502)
                     /locus_tag="RO454_000843"
     CDS             complement(15714..16502)
                     /locus_tag="RO454_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDY0932718.1"
                     /translation="MKIGTQNQAFFPGNILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDHRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            complement(16520..17572)
                     /locus_tag="RO454_000844"
     CDS             complement(16520..17572)
                     /locus_tag="RO454_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDY0932719.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17603..18445)
                     /locus_tag="RO454_000845"
     CDS             complement(17603..18445)
                     /locus_tag="RO454_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDY0932720.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18432..19313)
                     /locus_tag="RO454_000846"
     CDS             complement(18432..19313)
                     /locus_tag="RO454_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDY0932721.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(19334..20626)
                     /locus_tag="RO454_000847"
     CDS             complement(19334..20626)
                     /locus_tag="RO454_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0932722.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQR"
     gene            complement(20640..22319)
                     /gene="ycjM"
                     /locus_tag="RO454_000848"
     CDS             complement(20640..22319)
                     /gene="ycjM"
                     /locus_tag="RO454_000848"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDY0932723.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(22530..22844)
                     /gene="pspE"
                     /locus_tag="RO454_000849"
     CDS             complement(22530..22844)
                     /gene="pspE"
                     /locus_tag="RO454_000849"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDY0932724.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(22919..23140)
                     /gene="pspD"
                     /locus_tag="RO454_000850"
     CDS             complement(22919..23140)
                     /gene="pspD"
                     /locus_tag="RO454_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDY0932725.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(23149..23508)
                     /gene="pspC"
                     /locus_tag="RO454_000851"
     CDS             complement(23149..23508)
                     /gene="pspC"
                     /locus_tag="RO454_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDY0932726.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(23508..23732)
                     /gene="pspB"
                     /locus_tag="RO454_000852"
     CDS             complement(23508..23732)
                     /gene="pspB"
                     /locus_tag="RO454_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDY0932727.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(23786..24454)
                     /gene="pspA"
                     /locus_tag="RO454_000853"
     CDS             complement(23786..24454)
                     /gene="pspA"
                     /locus_tag="RO454_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDY0932728.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            24621..25598
                     /gene="pspF"
                     /locus_tag="RO454_000854"
     CDS             24621..25598
                     /gene="pspF"
                     /locus_tag="RO454_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDY0932729.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            25752..25889
                     /locus_tag="RO454_000855"
                     /pseudo
     CDS             25752..25889
                     /locus_tag="RO454_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            26023..26268
                     /locus_tag="RO454_000856"
     CDS             26023..26268
                     /locus_tag="RO454_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDY0932730.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            26581..28224
                     /gene="sapA"
                     /locus_tag="RO454_000857"
     CDS             26581..28224
                     /gene="sapA"
                     /locus_tag="RO454_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDY0932731.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            28221..29186
                     /gene="sapB"
                     /locus_tag="RO454_000858"
     CDS             28221..29186
                     /gene="sapB"
                     /locus_tag="RO454_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDY0932732.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            29173..30063
                     /gene="sapC"
                     /locus_tag="RO454_000859"
     CDS             29173..30063
                     /gene="sapC"
                     /locus_tag="RO454_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDY0932733.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            30063..31055
                     /gene="sapD"
                     /locus_tag="RO454_000860"
     CDS             30063..31055
                     /gene="sapD"
                     /locus_tag="RO454_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDY0932734.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            31057..31863
                     /gene="sapF"
                     /locus_tag="RO454_000861"
     CDS             31057..31863
                     /gene="sapF"
                     /locus_tag="RO454_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDY0932735.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(31911..33074)
                     /locus_tag="RO454_000862"
     CDS             complement(31911..33074)
                     /locus_tag="RO454_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDY0932736.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(33083..34456)
                     /locus_tag="RO454_000863"
     CDS             complement(33083..34456)
                     /locus_tag="RO454_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDY0932737.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(34460..37567)
                     /locus_tag="RO454_000864"
     CDS             complement(34460..37567)
                     /locus_tag="RO454_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDY0932738.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(37567..38688)
                     /locus_tag="RO454_000865"
     CDS             complement(37567..38688)
                     /locus_tag="RO454_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDY0932739.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(38837..39403)
                     /locus_tag="RO454_000866"
     CDS             complement(38837..39403)
                     /locus_tag="RO454_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0932740.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            39881..40669
                     /gene="fabI"
                     /locus_tag="RO454_000867"
     CDS             39881..40669
                     /gene="fabI"
                     /locus_tag="RO454_000867"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDY0932741.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            40814..41941
                     /locus_tag="RO454_000868"
     CDS             40814..41941
                     /locus_tag="RO454_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDY0932742.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            42009..43943
                     /gene="rnb"
                     /locus_tag="RO454_000869"
     CDS             42009..43943
                     /gene="rnb"
                     /locus_tag="RO454_000869"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDY0932743.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(44020..44123)
                     /locus_tag="RO454_000870"
                     /pseudo
     CDS             complement(44020..44123)
                     /locus_tag="RO454_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44177..46162
                     /gene="pdeR"
                     /locus_tag="RO454_000871"
     CDS             44177..46162
                     /gene="pdeR"
                     /locus_tag="RO454_000871"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDY0932744.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            46310..46483
                     /locus_tag="RO454_000872"
     CDS             46310..46483
                     /locus_tag="RO454_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932745.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            46572..47321
                     /locus_tag="RO454_000873"
     CDS             46572..47321
                     /locus_tag="RO454_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0932746.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            47590..47808
                     /gene="osmB"
                     /locus_tag="RO454_000874"
     CDS             47590..47808
                     /gene="osmB"
                     /locus_tag="RO454_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDY0932747.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT        11764 a        12681 c        11644 g        11812 t
ORIGIN      
        1 taatcaactt aatgatcaat atctatagtg atatcaccaa ccgttaaaac gaaccgacaa
       61 ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca ataatccaac
      121 catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc
      181 ggcaattgtg gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc
      241 cgaaaaactg gcggaaaaag gatttgtcac tattgcttat gacgcatctt atcaaggtga
      301 aagcggcggc gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc
      361 cgtgattgat tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg
      421 aatttgtgcc ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc
      481 catcggtact gttagtgcgg tcaatattgg ttcgatgttc cgtaatggtt gggaaaacaa
      541 tgtgaaatct attgatgccc taccatacgt tgacgcgggt tcaaacgccc gaaccagcga
      601 tattagcagc ggagaatacg ccactatgcc actggcacca atgaaggagt ccgatgcgcc
      661 gaacgaagaa ctgcgtcagg cctgggaata ttaccacacc cctcgcgcgc agtatccaac
      721 agcaccgggt tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca
      781 tatggcggaa gtgtacctga ctcagccgat acagattgtg gcgggcagcc tggctgggag
      841 caaatggatg agtgacgacc tgtacgatcg agcatcaagc caggataaac gctatcacat
      901 tgttgaaggc gcaaaccata tggatcttta tgatggcaaa gtctatgtgg ctgaagtaat
      961 ttcagtatta gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgtaat
     1021 agtgatatgg cctggcatat tgctgccaat aatctgggaa aagtttcatg aaaaatgtac
     1081 tgattcttgg tgctggcggt cagatagccc gccatgtgat taatcaactg gcagataaac
     1141 aaaccattaa acaaacttta tttgcccgtc agccagcaaa aatccacaaa ccctatccaa
     1201 ctaatagcaa gattattatg ggggatgtac tgaatcatgc agcactgaag caggccatgc
     1261 agggacagga tgttgtgtat gccaacctta cgggggaaga tttagatatt caggcaaatt
     1321 cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg
     1381 gtatttatga tgaagttcca ggaaaatttg gcgaatggaa taatgcagtt attggtgaac
     1441 ccctaaaacc attccgcagg gctgcggatg caattgaggc ttctggactg gaatatacaa
     1501 ttcttcgccc tgcctggctg actgatgaag atattattga ttatgagctt acctcacgca
     1561 atgaaccatt caaaggtacg atcgtatcac gtaagagtgt cgcagcatta atcacagata
     1621 tcatcgataa acctgagaaa cacattggtg agaatatagg catcaatcaa cccgggactg
     1681 atggtgataa gcccttcttc atgtaaatca attatatagc taccatcgta ctgtactatg
     1741 tgcagtacga tttattaaac ttattaaggt gaactgatca atcagatgcg attaacatgt
     1801 gtagaaccag taagaataaa atacgccatg tatataacat tacgacaatt ctaatatctg
     1861 cacatcaatg aaaatgcaaa caacttccgg caaaaaagtg atacaagatc gatgacatag
     1921 cgttgaagaa aaggatttga taatccgcaa aatttgcaca acactgcctg taatacttcc
     1981 tgaaccagag acgatcatga ccgtaactcg cccacgcgcc gaacgcggcg catttccgcc
     2041 cggaacagaa cattacggac gttcattatt gggcgcgccg ttaatctggt ttccggcccc
     2101 tgcagccagt cacgaaagtg gtttgattct ggctggcact cacggtgatg aaaactcttc
     2161 gatcgtcacg ctctcctgtg ctctgcggac tttgacacct tctttgcgcc gccatcatgt
     2221 ggtgctgtgt gtgaatcctg acggctgcca gttagggtta cgggccaatg ctaatggtgt
     2281 ggatttaaac cgaaactttc cggcagcgaa ctggaaggaa ggtgaaacag tttatcgctg
     2341 gaacagcgcc gctgaagaac gtgatgttgt attactgaca ggtgacaaac ctggctccga
     2401 acctgaaacc caggcgctgt gccagcttat acaccgcatt caacctgcct gggtggtctc
     2461 attccacgat ccgctggcct gtattgaaga tcccagacac agcgaattag gtgaatggct
     2521 ggcccaggcg tttgaattgc ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt
     2581 cggcagttgg tgtgcagatc tcaacttgca ttgcatcacc gcagagtttc cacccatctc
     2641 ctccgacgaa gccagcgaaa aatatctctt tgcgatggca aacttgctgc gctggcatcc
     2701 taaagatgca attcgcccgt cgtgaactga agcgccggtt ccacatctac cgccagccag
     2761 gtcggtccgt caagatcggc gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg
     2821 gcacgagagg tacacaacat gcagcccagc atcagacgaa aaccttgtgc acgcgcttca
     2881 gtcgccagcg ccagcgcttc cgtcagacca ccggttttat caagcttaat gttaaccatc
     2941 tcatagcgcc ctttcagcgc cttcagattg ctacgagtat gacaactttc atcagcacaa
     3001 atcggtaacg gatgaataaa attctccagc gccgcatcgt cctgcgcagg aagcggttgt
     3061 tcaagcatcg caacgcctaa atccgccaat agctggcaac gcgccgccaa cccttctgca
     3121 cgccaggatt catttgcatc aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc
     3181 accatccgct cactgataag atggttatcc agcttcactt tcagtaattt cgcgcctgcc
     3241 tgccagagtg ttgatgcact attggccatc tgatcaggcg taccgatgac tatcgtctgt
     3301 gccgtggtga ctgtctcggg aagcgttatg ccgatcaaat cagccagcga ttgctgctgt
     3361 tttcgcgccg ccagatccca caatgcacaa tccagcgcat tacgtgctgc gccggcaggg
     3421 aggatttttt gcaactcctc ccgtgtcagc cctttctcta gttgcggcac gacgctcata
     3481 atttgcgcca ttaccgaggc atcgctttcc ccataacgcg gatacggcgt gcattcaccg
     3541 gtgcctttga taccctcttc ttccagttca accaccacca cgcgcgcttc actgcgactt
     3601 ccccgagcaa tcacaaacgg ggtatgtaag ggccaggcct cctcaaatac cttaacggtt
     3661 ctcatcttta ctccttgttg tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg
     3721 tgttattgac agttagcata aactaggtgt gacgttaact atatgtaaac aggaaagata
     3781 atcatgtcac aaaccgttca tttccagggc aacccggtta cagtcgccaa ttccatcccg
     3841 caggcgggta gcaaagcgca gacttttact ctcgtggcaa aagatctgtc tgacgtcacc
     3901 ctcggtcagt ttgcgggtaa acgcaaagtg ctgaacattt tcccgagtat tgataccggt
     3961 gtttgcgccg catcagtacg taagtttaac caactggcaa ccgagatcga caacaccgtt
     4021 gtgctgtgta tctctgccga tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt
     4081 ctgaacaacg ttatcaccct ctccactttc cgtaacgctg aattcctgca agcctacggt
     4141 gtggcaattg ctgatggccc actgaaaggt ctggcagcgc gtgccgttgt ggttattgat
     4201 gaaaatgaca atgtgatttt cagccagctg gtggatgaaa tcaccaccga gccggattac
     4261 gaagcagctc tggctgtact gaaagcataa gttaatatgc ctgatggtat tgcaccatca
     4321 ggcatattca cgcttactct tcgttcttct tctgactcag accatattcc cgcaacttat
     4381 tggcaatcgc ggtatgtgaa acgccgagac gttttgccag tttgcgcgtg ctgggataat
     4441 tgcgataaag ctgggttaat accgagcgtt caaaacggct ggtgatttcg tccagcgaac
     4501 cttccatcgc atcttcgccc acggctaccg ttgcggcgtc ataatccggc aacaaaatat
     4561 cctgtggacg cagctcataa ccgtccagtt gtgtcagtgc acgatagata gcgttcttta
     4621 actgccgcac atttcccggc cacgcataac gcgtaagtac agtattcaga tcagcggcca
     4681 gtttcggacg cggcacgccc tgctcgtcgg caaagcgggc gacgaacagc tcggttaacg
     4741 gcatgatgtc ctgcggacag tcacgtagcg gcggcagatt gagcgtcaac acgttcagac
     4801 gataatagag atcttcacgg aacacgcctt tttgcaccag ttcgaccaga ttcttctgcg
     4861 tagcgcaaat cacccgcaca tcgacatgca cctcatggtc ttcgccaacc cgacggaagg
     4921 tgccatcatt aaggaaacgc agtaatttcg cctgcatccg tggtgacatc tcacctattt
     4981 catccaacag caccgaacca ccgttcgcct gctcaaagaa ccctttcttc ccttccggag
     5041 catgaccaaa cagttcactc tcgaccgcat cttccggtat agacgcacag ttcagcgcca
     5101 ggtaaggttt gctcgctctg gggcttgcct gatgacaggc gtaggcaaag agatcttttc
     5161 cggtgccggt atcacccgta atcagcagtg gcgcgcttag catcgccagt ttctgcgcct
     5221 gttcgacaac atgcttcatt ttcgggctga cagcgacaat ttgactgaag gcgctgacat
     5281 cctgggcggc gacattttgc aactgacggc ccatacgaat cgttgatcgc agcatcacca
     5341 ccgcaccggt caggacgtgt tgatcatttt catcctgaag ataaacaggc gtaatctcca
     5401 tcaggaaatt ctgcccatta ataacgacat gctcgttatg cgaatcttgc ggttcgcttt
     5461 ccagccagcg taaaaaatta aagccgttaa tcaattgcgc ggcggtatgg ttgcgcaggc
     5521 gatccaattt ttgcccaaaa agctgacagc tcgccgggtt cgccatatcc actttgcttt
     5581 tcatatcgac agagagcaca ggttcaggca acgcctccag taacgcgctc aacgccagat
     5641 gctcacgttc ggaaggcatc cacgggacag tacgcacatc ggtaacacct gcaatacggc
     5701 gtatttcggc catcagactg ctgaaactct caaactccag ttcagcaaaa ttgaggtaga
     5761 ttcgcccaat ggggtcaatc tcaataccgc gtaaatcaat gcctcttagc acgagtagat
     5821 cgagtaattc gcgggtcaga ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa
     5881 ccttcacctg aaaaaagaac aattaatatg atgatatgac actatttgga tagcaggaag
     5941 gtttctgtca acaatcattg acacaaagac ggaaaaacgg gcacttcggc gtaaagatat
     6001 ccggtcagca cccgatattg cattattttt cgctgggcgt tttgcctttt tgcagagttt
     6061 ctttcacctg accgataagc tgacgacgga aatccccgag gcgtggcttg tcatcgtcaa
     6121 tccacggcag cgggcggcaa agctccatag ctttaatccc gagtcgtgcc gtcagaagtc
     6181 cagcaccaat cccctgagct gcccgggtag acaaacgcgc agcgagatct tgcgacatcc
     6241 agtccatccc cacttcgcgc accagttcgc tggctccggc gaaagcgata ttcagcaata
     6301 ccagcttaaa caggcgcaaa cggctgtaat atcccagttc aatgccatac agcgtggcga
     6361 tgcgattaat caaacgcaga ttgcgccagg cgataaacgc catatccacc agcgccagtg
     6421 ggctaacggc aatcattaac gttgattccg ccgctgagcg gctgatttcg cgccgcgcct
     6481 gggcatctaa aactggctgg accaggtgag catacaaact gaccacttca cgatcgttct
     6541 gcgtttcatg gattgaggca taccagcgct gcagcgccgg atgtgactga tcgatgcccg
     6601 cctgctgcgc cagtttttcg cagaatgcac ggcctttacc cgtgccgtga ctgtgcaaca
     6661 aatcgcgcgc ttcatcgcgt tcatgggcgc gctgtcgcaa gcgccataag cgccgccact
     6721 ctgttaccac agaacctacg ccagcgccga taatcaatgc cccagcggca catccaccca
     6781 gcgccaccca gtcctgggtt tgccaggcgt tcattgtcca ctgcacgccc tgcccgacaa
     6841 cgcttgcgcc aaacagagcc agcccgccca tcaccatttt gcgccacagg ctgcgtttcg
     6901 gacgtaacgc tgcgtccatc accgcttcaa cttgcccctc ttcttcctgt gcttcgtcga
     6961 gcgtggccgg ggcaaaattt tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt
     7021 ttggattctg atcgacctcc agtggaccgt cgaaatcaat acgtggtttt aacggttcgg
     7081 tcatcgcaat ttatctccta ttaaaaattc cagcgcagca tcaagacgaa tatgcggtag
     7141 cggtttgtcg acatccatca cctgcggacg aaacgcctca aactggaagc cttgcttatc
     7201 ccagaacgcc tgacctggca aacgtgcggg aacttcgcca ggataaacag tgagcggtgc
     7261 gccatcgctg agtcgattac cgcgcagcgc cgggattttc tcaccgttaa catcaatcat
     7321 tccgctggtg gtcgcctgaa cagacgccag ccccaggcag tccatactga tcccttcgaa
     7381 cgccgcattt tgccatgcat cctgaatcag ttgttgcagc aatgaaacca tattggcgtg
     7441 ctgatcgatg gtcacatggt ccgctttagt ggcagcaaac aatagcttat cgataaccgg
     7501 cgaaaacaaa cgccggaaca gggtacgctg cccgtagtgg aaactttgca tcagctgcgt
     7561 cagcgccaga cgcatatcat taaatgcctg tggcccactg ttgagaggtt gcaggcaatc
     7621 caccagcaca atctggcggt caaagcgcag aaaatgattc ttatagaacc ccttcaccac
     7681 tttctcgcag taataattaa accgctcgcg caacattccg gcattagtgt gcttatcggc
     7741 ctgcgccagt ttggactcgc cccaggcatc gacatccggc cacgggaaga attgcagcgc
     7801 gggtgctcct gccatatctc ccggcaagac aaagcgccca ggctgaataa agtgcaaccc
     7861 ctgctgtttg cagtgatgga gatactcggt ccaggcggcg gcaatgtctg ccagccggtt
     7921 ttcgtcggca ggcgctagcg ggtccagccc ttcgcacatc attcgccatt tcgccgacca
     7981 ttcgccgcgc tgaccattga gtaagcccgt catctggcgc gaccagctta aatagtcctg
     8041 cgccagcatc ggcaggtcga gcaaccattc gccggggtaa tccacaatct ccagatacag
     8101 cgtggaggta tccttaaagt ggcgcagcag cgaatcgttc gatttaaaac gtaacgccag
     8161 gcgaatttca ctgacaccgc gcgtaggcgt cggccaggcg ggaggatcgc catataactg
     8221 cgccaaccct tcatcatagg tgaagcgcgg aatgccaaag tcacgctggg gaatgcgttt
     8281 tacgcccagc aggcgctctt cacgcaccgc acttaaaagc ggcaaacgtg ctccggcatg
     8341 aatattgagc aactgattaa ccatcgcggt gataaacgct gttttgccgc tgcggctaag
     8401 tccggttaca gcgaggcgca gatgtctgtc gacaccccga ttcaccagcg cattgagttc
     8461 atttttaagt cgcttcattg ctgtcctgtg ctgctctgaa gaccagaaaa aaaatcgttc
     8521 gtaaggatat gaatgtgggg gccaggccat tgattatcaa ctacccgtgt tgtcatcgac
     8581 gggcgaactc cataagatgt gcagcattca cgttcggacg gagttcctga cctatgtccc
     8641 ctactattta tgatattgcc agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc
     8701 tgaataagca aaccaatatc tccccggaag cgcgcgaaaa agtgttacgg gccattgaag
     8761 aattacagta tcaaccaaac aagctggccc gcgcgctgac ctcttcgggt tttgatgcca
     8821 ttatggtgat ttctactcgt tcgaccaaaa ctacggcggg taatccattt ttctctgaag
     8881 ttttgcatgc catcaccgcc aaagcagaag aagaaggttt cgacgtgata ttacaaacgt
     8941 cgcacaatcc ggcagaagat ctgcaaaaat gcgaaagcaa aattaagcag aaaatgatta
     9001 aaggcattat tatgctaagt tcgcctgccg atgaatcctt ctttgcacaa ctcgacaaat
     9061 acgagatccc ggtggtggtg atcggtaaag ttgaaggtca atacagccat gtttattccg
     9121 tcgataccga taattatggc gacagtattg cgctgaccaa tgcgttaatt gaaagcgggc
     9181 ataagaatat agcctgcctg catgcgccgc ttgatgtaca agtttcagtg gatcgggtaa
     9241 atggttataa gcaaagtctg gctacgcata atattgcagt gcgtgatgaa tggattgttg
     9301 acggcggtta tacccatgag acagcattaa aagcagcacg ggaattatta agccagtcac
     9361 cgttgcctga ggcagtgttt gctactgaca gcctgaaatt aatgagtatt tatcgtgcgg
     9421 cggcagagaa aaacattgct attccgcagc agttagcggt ggtgggttat agtaatgaaa
     9481 cgctgtcgtt tatattaacg cctgcaccag gcggcatcga tgttccgacg caggagttag
     9541 gtcaacaaag ctgcgagtta ttattccagt taattgccgg aaaaccgtca ccacaaaata
     9601 ttaccgttgc tacgcatatg tcgttgaaat aaatgtatgc cggatttggc gctcgccccg
     9661 catccggcaa tttagcacat tatcagaacg agtaattcac cccaacaccc gcataatgga
     9721 atttatcgct gtcgccttcg tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg
     9781 aaagaccatt ctggaaatca taaccgtaaa ataaacccac acggttaaaa tcatggcctt
     9841 cacgttcaat atcatcctgc cagtcccagt tactccagcg atccagccca atgcgcgtat
     9901 acggcgtcac cgagtggttg ccgagcgtca gcggcaaata ggcgcgaatt tcttgcgtgg
     9961 aaaactcacc gttattgcgg ctatcatcca tattgaaacc acgctcgaga taatagttca
    10021 ctcgcaaggc aaccgtttcg ttgaaggtat attgcagacc cgtttccgtt tcgacacggg
    10081 tatcagcata accggtcgta ttcagatcgt tggcaaattt atacatcgac agccaaccgt
    10141 tgaaacgtaa atcgtcagtc agtttcacat cccagtctgg cgcgattttc cagcgctgca
    10201 tatttgccgt gtctttaccc ggttcatcaa cgtagtgata accatagtta cggaaaccac
    10261 cagtcagacc gagactgaaa tcatcgcttt cgaggaactg ataatgtacc tccagctccg
    10321 ggcgatcaaa ccacgttcca cgtttacccg cgctataatc taccggccct tcctgataat
    10381 aggccagaga aattctccac ggcccgttgg cggcattaaa atagacagaa ggctctgcca
    10441 gcccatccat atcttcgaca taaccctcga cgttttctat ttcgtacatc gcgccgatat
    10501 taaagtgcca gttgttcttt tcctcggcct gtgcgcaggc cattcccgca cacatcacca
    10561 gtgcggtaca gggtaataac tttttcatta ttgttccttg tattatcctg ggagaatgcc
    10621 ttccccctgt atttagcaaa ttgctatttc cgtttctgca tcaaagaaat gacatttagt
    10681 catatcaaaa tgaatagtga tattttctcc tgcatgataa tcatttaacg caccagcacg
    10741 gaccactaac tcgtgccccc caaccgtggt gtagagcata aattccgcac cggttaattc
    10801 cgccacgcta attttggcgg aaatgttatt ttcttcttgc gcatccggat gaatatcctc
    10861 cgggcgtatt cccatcacaa tgggtttatg taaactttcc tgcgttttca gaaccgctaa
    10921 tttctcttcg ggaatggtta atttaagtgt ttccgtaacg aatttatcgc catcgatcgt
    10981 gccgcgaata aaattcattg ctggtgaccc aataaatccg gcaacaaaca tattcgccgg
    11041 ttggttatag accgttttcg gcgcaccgac ctgctgaaca atcccatcct tcataatgac
    11101 aatccgcgtc gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt
    11161 gttcagtttc tgatgcagct tgctgatctc tgcgcgcatt tgcacacgca gcttggcatc
    11221 caggttagag agcggctcat ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc
    11281 aagcgccact cgctgacgtt gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc
    11341 acgcaggccg aggatttgcg ccgcccagtt aacccgctca tcaatcatct ctttggcgat
    11401 tttttgtatc ttcaggccaa acgccatgtt gtcgtagacc gtcatatgcg gatacagcgc
    11461 gtagttctgg aacaccatcg cgatattgcg tgctttggct ggaacgtcat tcattcgttt
    11521 gccgtcgatc aacagctcac cgccgctgat ctcctcaagc ccggcaatca tacgcagggt
    11581 ggtcgattta ccgcagcccg acggaccgac aaacacgatg aactctttat cggcaatctc
    11641 caggctgaag tccttcacca catgcacctg gttatcgtag atcttttgaa tatgttgtaa
    11701 cgaaagctgt gccatgttga ttcctttgct atacgttttg ccagaaggcc gataaccgcg
    11761 gccaggtcag ggagtccgtt gagggcaaca ataattgcgc cccggttaag cccgcgccga
    11821 tccccaccga gcgcataccg ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa
    11881 tgccgataca tgcctgtggc ggcacgccca gccctgcaca ggcggcgaga aagatttccg
    11941 ggtccggttt cgaatttttg agttgggaag cgtccgcgca gaaggtgaaa aactcgcgca
    12001 gctccagcgc tgctaaaatc gtcggcgcat tcagggagac agaagccagc ccaactggaa
    12061 tctgctgtgc ccggagatct accagcaaat tgtgaatgcc aggcagcaca gcattaaccg
    12121 tcagttcgcg cagtgagtgg acgtaaagca gatttttgcg atacgccagt tgcgcccgtt
    12181 cctgtgggtt aaaatcgccc tctttacccc cgtgctgcag aattcgccgc agggactcat
    12241 cgcggctgat cccttttagg aattcgttaa actgcgcatc aatgctgatg ccaatttcag
    12301 cggcaatctg ctgccacgcc tggaaatgca gatgcgcggt atcggtgatt actccatcca
    12361 gatcgaaaat taccccttgc agtttcattc accctcctga tgcgtggtag cggtcccatt
    12421 gaagggcaaa ataaaatccc ccaaacagaa aacagattct tcagcgacgt aaataagctg
    12481 accgttcaaa cgcagtgaaa caggcgcaga agttcgaatc gcaatacgct gcgcgtcgag
    12541 agtgacctgt aattcgcagc cctgccagaa cagagggaaa gacaactgtt gccactgctc
    12601 aggtaacgcc ggattgagat gtaattcacc gtcacgcacg ctcaccccgg caaaaccctg
    12661 aatcgccccc agccagatag cgccagttgc ggcagcgtgg ataccgtcat cacagctatg
    12721 cggatcagca ccaagatcga tttcagtccc ctcgcgccag aactgataac tttgggtcag
    12781 caggccacag cgtgcggcaa caatgccgtg gattgcttta cttaatgacg agtcgtgaat
    12841 agtgcgcggt tcataaaatt gcagattggc aagacacgat gccgctgaga actgctccgg
    12901 cagcatgtaa ttgagcatca ccacatcagc ttgtttgagg atctgcatct cgttcacttc
    12961 tgcgcgtgaa taatccagca gaatggtttg cttccccgcc gccgctttgt atttcgccag
    13021 attaatcacc ggcttagcca taaacgaatc atcctgcggc aaaacgccgt cgggctgaat
    13081 ttctggcagc cgcagttctt tgaggtacat ttctgcacga tgaataaacg cgtcgtcgct
    13141 acagccaaac tgacgagcga tattcagcgc ctgctggacg ttgtaatacg ccatatagct
    13201 ggtgaaggcg ttattattga catgttcggt atattcgtct ggcccaataa catcatgaat
    13261 ttccagacgc tcgttaaccc tcaccgcgcg gctaatccag aactttgccg tctccagaag
    13321 tagcgccatg ccttcatgag caatgaaact ttcatccccc gtggtccgcc agtattgaat
    13381 caccgcccag gcgatatcgg cgaccagatg gtgttccgcc tgcgcagagg ccactttttg
    13441 ccgcagcccg gtacgaatgt taatggcggc aaattccggc gtctcttctt cgccgctacg
    13501 cgcgctttcc cacggaaaca gagcgccctg ccagccgttg cgtcgcgctt tctcctgcgc
    13561 gccaggcaag ttgtgccagc gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa
    13621 cagatggaac ggtagcaaaa atacttctgt atcccagaaa acgtggcctt tgtagccttc
    13681 gccggttaaa cctttcgccg caatgctgct gcgctcatcg tgggcaggcg tcatgatgcg
    13741 caggtgataa agcgcataat ctaatgcttg ctgatcgtgc gcatcgcggc cgtttaccgt
    13801 gatacgacgt ttctgccacc attgacgcca gttttctgtt gatgctgcaa gaagttggtc
    13861 gtaactctgc tgcgcgcaca tttcaagctg acgaagcgac gcactgcccc attcgtctaa
    13921 agcggcttgt ctgtcgtccc gccagtcgat ccacaccagt ttttgcagcg tcagcgtctc
    13981 acctgcgtga agctgcgcgc aggtatgttg caataaacgg cgctctttgg cggtatagca
    14041 ttgctgcaca tcaccgctca ccttacagca acagctgatg gccacatcac tacgcccatc
    14101 ctgggtggtg tagatcccct gcatcagatg ctgaccaaac acccgcacct ggatttcgtc
    14161 aatatgctgg cgaccgtggt tggtttgcgt ggcgtcaatg cctgttgaaa tcagcactga
    14221 cgcgtcggcg tccagtggag taatagtgat ttccagcgca atgagcggca gttggtctgc
    14281 cgaaacaaag cgacggctgg cgatggtgta accagtgccg ttgctggtac gccagacaac
    14341 gttgcgacgt aattcgccac tggcaaagtt aagctcacgc tgccatgctt cgcgggataa
    14401 cgagaaaatc tcaccattta tggcaatctc catccccacg acatcaggca ggttcaccag
    14461 ttcgttgatt tctcctttac ccgctcgatg atacagcccc gccagataca tcccacgggt
    14521 ctggcgcgtg taatcttctt catggctggc acgaagccca agatagccgt tcccctgcgc
    14581 catcagcgaa gcatacttgt tcagggtatg ctggctgaaa tggggttctg ataacgttac
    14641 tggcctggtc ataattccac acgcgtccct gtttccgcgg attgatacaa cgccgccacc
    14701 agttgctgga tgatgtaccc ctgctcggcg tcggctatca tcacgggctt gccctgtacg
    14761 tgattgataa aggcttccat gctgcgcaaa tggcggttgt cgtctgccat ttcccgttgc
    14821 atcagcgtca ttaattcacc gttgttatcg gtgtagatat gtgccggaaa cagcgtcgca
    14881 ccggctttat caccacaaaa gctgacattc ataatcgact gttcgcggat gttgagtgca
    14941 aatgacgttt ccagccacag aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa
    15001 tcttcaacgc tgtaagttgc cgggtcccac tcgccaaatt gaccacagct cttttgcgtg
    15061 ccgatctttt gaaagctatg cgcattcacg cttttcaccg ccggaaagcc cagcacatac
    15121 atcgcagcat ccagcatatg aatgccgatg tcgatcagcg ggccaccacc ctgcagttct
    15181 ttattggtaa agacacccca gccgggaacg ccgctgcgac gcagggcgcg ggcggtggta
    15241 acgtaaatct ctcccaaaac gccgttggtc acctgttcac gcagctgttg cgtatcgaac
    15301 gcaaaacgat ggtgaaagtc gtaggccagc accttgccct gtttgcgcgc ggtatcgcac
    15361 atttcccgcg cctgttctgc cgtcatggcg ggcggttttt cgcacatcac atggcagccc
    15421 gcttcaagcg ccatcagggt atgttcgtaa tgaaaacggt tcggtgagca gacgctaacc
    15481 acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacaagga tgcattaccg
    15541 tatttttctg ccagcgcctg cgcctgggaa aggcggctgt cacagacggc caccagttcc
    15601 agatcgttgc gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcctgcgccg
    15661 attatcgcga cacgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag
    15721 gtgcgcaacc aggccaacga atcacggtac gcctgggcag gagcttctgc ccggatacgc
    15781 ccttcataca ccacataacc ctgatagtta tcggcgcgca gctgttcaaa cagcgcgtgg
    15841 aaatcgaggg tgccgctgcc cggctggtaa cgatggttat cggcaatatg cacatgaccg
    15901 agcaagtcgc ggtggtcatg cagcgcctgc gccaggttat cttcttcgat gttcatgtga
    15961 tagaaatcgc cgataatctg cacatgttta agatcgtttt cgacgatgta acggcgggca
    16021 tcggcgagag tattgatcat atgatcctga taacggttta acggttcgag atacaccacg
    16081 gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cattttgcgg
    16141 tcgccgtcca ggctgcgcgg cgaggtcatc ggcggtaaac ggaaggtaaa catgccccat
    16201 gcagcaggaa cgacgatacc tttaccgccc acttctgcca gcgcttcgag aatgcgttcg
    16261 atctgcttta agccattaag acgacgctct tcgataaaat caccaatcca gccgtcatag
    16321 ccgccgcagg cagtggtcac cggtaaaccg gtttctttga tcgccgcttt gacttcttcg
    16381 atgttgttga ccagcagttt gccgtcaatc tcaaaaccat cgaagcccat ctctttgatg
    16441 taacgaaatt tctccagaat atttcccgga aagaatgcct gattctgtgt gcctattttc
    16501 attttttcat tcctttgaat taaaacgtaa cgcccatttt gatgctctgt tccggatgct
    16561 gatcgacata ctgcatgtag ctttccgggc tgttggcaaa ggtcactacc gggtcgatta
    16621 aatcttcgca attgagataa ccgttcatca gcagttccca gcaggtttct tcaatacgct
    16681 tacggctcca gcgcggataa tccgggttcg gttcgctgca cgcgcgggag aaaacaattt
    16741 tggcgttatt gaaatgcgct tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa
    16801 acgcgacata ggagatggtg ccgccgtagg ccagaccgcg gagcgccgac tgcagcgcgt
    16861 cggcgtagcc gctggtttcg ataatcacat cggcaccctg cttgccggtc agcgttttga
    16921 tctctttacc tacgtcagcg ccaatggggt taagacagaa atccgcaccg tggcgacggg
    16981 caatatcaca gcgatgagcg ataggatcga caccaatcac caccgacgcg ccagcgcgtt
    17041 ttgccagttg aatggcaatt tgaccaatcg cgccaagccc taccaccacc acaaaatcgc
    17101 ccacgcgcac gttggcatcg cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaga
    17161 cggcgttttt ccaggagctg ccttgcggca ttttgcgcag cttgtagtta ttcactgcgt
    17221 taatgatgac cgtctcggag agcgggccgt agccgcatac gctgtcgccc accgcgtagt
    17281 cgttaacgtc gctgccgcac tcgataatgt cgccaaccac catgttgcca agctggaact
    17341 tgccaaactc aatgccgcgc ggcgcatttg ccggacgcgg gctgaacatc tgccattcac
    17401 cgttaaagtc ttcatcgata aacggactgg cagcgcggaa atcgaccact tccgtcccgt
    17461 gcttcggagc gccgaaacgc acgcggatct tcacttcatt ggctaaaatc gcccgctctt
    17521 catactcaac cagcgcagct acacgcggtg ctgtggctac taactttttc atgtaaaact
    17581 ccttgtaaag aaattggtcg ctttagccct tcacgccacc agcggtcaaa ccactcttaa
    17641 taaaacgttc cgaaagcgca tacataatca ctaccggtag tgcggtcacc agcgaggccg
    17701 ccatcatccg tccccagatg taatcaggcg tactgaacag cgcgttcagg cccacaggta
    17761 aggtgaaatt gctggcgctg gaaaggaaaa tcgacgcaaa cagatagtcg ttccacgcca
    17821 ccataaagca gtagacgaat acggatatca gcccggacat tgccaggggc acggtgatac
    17881 ggaagataat ttgcagacga ttgaggccat ccatcattgc cgcttcttcg atttcatcgg
    17941 ggatggtgtc gaagtagctt ttcagcataa acacggcggt aggcagagtt tgcgtcacca
    18001 tggtgataat cagcgccatc tcggtgtcgt aaatgccaag tgcggtgatg attttgaaca
    18061 gcggcaccac cagcaaaatg ccggagaaca tgtacaccgt gtaaaagctg gcgttgatgg
    18121 tcattcgccc tttaaagcgc aggcgggaaa gcgcgtaagc cccaagaatg ccgagaaata
    18181 ccgccaccac ggatgaaacc accgacacca ccatgctgtt acggaagtag tcgacaaacg
    18241 ggaaaatcac cggattaaaa atgtcgacgt aatgctccag cgtccactgc tgtggcagca
    18301 acgtaggatg cagcgagatc gcctcttttg cgctcttgaa cgaggtcatc agcatcacaa
    18361 aaaatggaaa cagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa ccgattcgac
    18421 tgagtgtgcg tttatttgtt gccattgagg ttcacccgtt tcctggtcag cagaatgaca
    18481 gcgaaaataa tgatgaaaag caccaccgag atcgccgccg ctttccctaa gtcattaaag
    18541 gcaaaggcgg ttttgtagag atagacaccg agaatatcga ccttggtcgt cagcagataa
    18601 acatccgcga acatgtagaa catccagata gtgcgcaggg tcaccaccgt cgccagaacc
    18661 ggcataatcg cgggcagcgt gacgatgcga aaccgttgcc aggcattcgc gccatccatc
    18721 tctgccgctt cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag
    18781 gcatacggga agtagcgcca gatggcgaac agcaccacca gcacaaaact actgcccggg
    18841 ttatcgaacc acagcggcgc ctgctcatag agatgcagaa gatcgacgcc gaggtagttc
    18901 acaatgccgt agccgttgtt gaacatgtat ttccaggcga acaccagcga aatagacggc
    18961 gttacgtagg aaaggatcac cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg
    19021 aaaaacatcg ccacggccag cccgagcacg gtgctgcccg ccaccacaag cgcggtatac
    19081 cagaccgtca tccacagcga gtgccagaaa ccaggatcgg agagaatgcg cacatagtta
    19141 ctcaccccaa caaacgttga ctcgatgttg ggattaagcg gcagacgtaa aaaactgatt
    19201 tcgatattcg acaccatcgg ccacgccacc agaccgccca gcagtaataa gctgggcgcg
    19261 agaagcaaca gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat
    19321 tcctgtcctg ggtttagcgc tgttcgacca actcatccag ctttttctgg ctcgcttgca
    19381 gcgtgccagg gaggtcggct ttacccacgg tgacgttatg caccattgaa ctcaccacac
    19441 cagaccccgt cacatcaccc atgcgggtaa agtttttatc ccctactgcg ccaaagacct
    19501 gaatatttgg caattcgctg attagctgat tcggcaattc tcccagcgcc ttaataacgt
    19561 cgttgtcttt ccaggtggcg gtagtgacaa ccgctttgtt caccggcagc gccgcgcctg
    19621 gtgacatcat cacccagtcg gcaatgttgt ccgcctgctc cataaaggtg acaaattttt
    19681 cagccgcttc ggtctcttca gtcttttgcc cggcggtaat ggtcagcgag gtcaacatgc
    19741 cgtagaccgc agaatttttc tcggttggca ccacgaaacc aacgtttttc ggatcgcctt
    19801 ctttaatcac agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca
    19861 taaaagcgtc tttcacttcc atgatgtcgt tagaacctgg catggtgttg gcagcaaggt
    19921 tgcggtaata ggtcagcgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
    19981 cgttaaagac gttagcctga ttggataacg caaactggga gaaagactgt tccgtcaaca
    20041 cgctttctgc tgtaggcagc gcaataccgt attttttatt cgccgggtca ttcagtttct
    20101 gtgcaacgtc cagcaattgc tgccagtttt ttggctcctc cagcccagct tttgccagca
    20161 catctttgcg ataccagatc ccgccaatcc atgcgctgac aggaacaccg gtccaggcgc
    20221 tgccatcttc ggtgcgcaca atccgcagta cgccatcgta gaacgcgcct tcaccgacgt
    20281 tgctgatgac cgtggcaacc gctttacgat cgataagctg ctctttgtcc atcactttgg
    20341 cgtagtcatg gctggtttcg atcacttccg gcagcgaacc gctacgtgaa agggtaatga
    20401 ctttggtgtt ataggcgtct tcttccacgg gcacttgttt tacggtgata cctgggtttt
    20461 ctttttcaaa acgggcaatc aatttgctga tgacggcctg gcgctcctgc tccaccgaag
    20521 aatgcataaa ttcgatggat acattatttt tattttcttc tttacagcct gaaatcaggg
    20581 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaaa tgttcctttt
    20641 tatttaatcc acataacctg ccagggatgc agtgttattt cattacccaa tatatcaact
    20701 tcactaatca gatcccgacc gtgcaggtca gtaatattta tatgctgaat attttcgctg
    20761 acattaaaca atcccgtcag gcaatcgcca tccgcattgc ttctttgaat tcgcattaca
    20821 aatgaattaa ttgcatcgat ggtgaaatta ttatccggat gaaactcttt atgactgcga
    20881 cgaagggtaa ttaaatgcga caacttataa tataccgaat accttaatgt cgctttattg
    20941 tccagttctg tcgttatctc tttactagag tattttttac ggttaatcgc gcgattatat
    21001 ccaagttttt cgacaccggc gtaatcatta cgcgacccca gaatgctttg aatatatatc
    21061 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcacaccgt
    21121 tcttcgtcgc tacactcacg gcggctcaac gcatccatat aggtgacatt catttcatag
    21181 gggctacgcg taccgtcggg attatttttc cagtttacta atgccccttc ctgctgtaac
    21241 acctcgacca gcgctaatat ttcgttttcg ggtaacaacc cccgcagtgg gttaagcccg
    21301 atgccatcgt gagaggcaag gaagttaaac caggtggtgt tgctggaagg tagtgtcagg
    21361 ttttgcgccc acgcacaaag tgcctcaacg ttctgttttt gcaccgcatg cagcaccagc
    21421 ggcggcagcg agaactggta caccatatgc gcttcgtcat cgccttcgcc aaagtaagca
    21481 atgttgtctt tatgcggaac attggtctcg gtaatgatca ctgtgcctgg cgcgacgtta
    21541 tcaataatcg aacgtaacag tttgataatc agatgtgttt tttccagatg gatgcagttt
    21601 gttcccggct ctttccacat aaagccaacg gcatccagac ggacatactc tgcgcccttt
    21661 tccaggtagc acagcaatac atccaccatc gccagcaaca cttcagggct acggtaattg
    21721 aggtcaattt gatcgtcgct aaaggtggtc cataaatgac gcgttgaatg atcgcgcatc
    21781 tggaatggcg ttaataacgg taacgcacgc gggcgagtga cggcgctgag atcggttttc
    21841 ggatcaacgg taataaaaaa atcttcaaaa cctggctgct gttgtaaata gtttttaaac
    21901 cattcacttt ttgccgacat atggttgcag acaaaatcaa acattaaatg gctgcattca
    21961 ccgagttgct gaatatcctg ccactccccc gcttcactgg cgacctgatg ataatcaatt
    22021 accgaaaatc catcatcaga tgaccatgga taaaaaggta ataaatgaac atgtgaaaaa
    22081 attctttgca gccattgagg gtaaaactga ttaaatgtgg gtagtggctt taaatcatta
    22141 ctgtgaaatt gatcggcata ggtaattaac acgacatcac tttcatccca gtgtttttta
    22201 cgtcgctgtg taattaatcg tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga
    22261 gtcgcggtaa atgttccacc gtagatttcg tccaggtaat ccgtaatttt ctgttgcatg
    22321 ttgcctttcc gtggtagcgg tcccatgaag cgagagacta ctccgcataa aataaactgt
    22381 caattgagct caaggggttt agtcacgcag gaaatctttt tttacctggg aaaaagcgta
    22441 tttgtgagct gacgcaaata atctatgggg atgtaaataa ggtgtgacag aaaaaaacgg
    22501 cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct
    22561 ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgcc
    22621 tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg
    22681 gcggtagcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc
    22741 tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc
    22801 agtgaaaaca ccagtgtcag agcaagtaag ccttttttaa acatgtaagc gtccttctat
    22861 ggatgaaaat tgctatccat tatacgcctg agatttaacc atttttgcga aactcccctc
    22921 accttttata acgctgcgcc agtttattgg cagcccgact taacagcggt tccagcgcca
    22981 cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac
    23041 ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaacccag
    23101 gcttcacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg
    23161 gaaacggcta cgcagcgtga aagtgtcgga agtgacataa cgttccatct cgcgtaaacg
    23221 cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag
    23281 ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta
    23341 agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac
    23401 cagttttact ggtacatcaa aatagttggc aatcccggcg cagacgccac ggaccatgcc
    23461 ctgctgcgga atacgccata attttttatt gagattaatg cccgccatta gcgatccctc
    23521 cagttcggat gttctgcatc aagaatggat tccagcgcct gaatacgctc gcgcatccgt
    23581 tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgtgacaa ttcactgcga
    23641 ccggaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg
    23701 gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc
    23761 gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt
    23821 gtgccagttg ctcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg
    23881 atttttgttt accgaagctg tggctttctg cttccgcttc catctggtcg atacggcgtt
    23941 cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca
    24001 catcgcgcga cgagtttgcc gcttgatggc gtaacatcaa tgcctgctgg cgagcgcgtg
    24061 tttcgctcaa tttgttttcc agctcgccga tctctttctt catacgcact agcgtatcgt
    24121 ccaccagcgt cacttcatgt tccaggctct taatcagatc ggtcagtttc tgtttttcaa
    24181 ttaacgccgc acgcgccaga tcctctctct ctttcagcag tgccagttcc gctttttcct
    24241 gccattcaat ttcacgagcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg
    24301 ccagcgcacg cgccgaagta gaacgtactt caaccaatgt atcttccatc tcctggatca
    24361 tcagacgcac cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat
    24421 tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt
    24481 caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt
    24541 tataaagatc tgattgaaga atcaacagca acatgccagg atgaattagc gaattacact
    24601 aacaagtggc gaatttcatc atggcagaat acaaagataa tttactcggt gaggcgaaca
    24661 gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca
    24721 tcatcggcga gcgcggcacc ggtaaagagc tgattgccag ccgtctgcat tatctctcct
    24781 cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa aatttgctgg
    24841 attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatccag
    24901 ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga
    24961 tgatggtgca ggaaaaatta ttgcgtgtga ttgagtacgg tgaactggag cgcgtcggcg
    25021 gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg
    25081 caatggtcaa tgaagggact tttcgcgctg acctgctcga ccgactagcg ttcgatgttg
    25141 tgcaactgcc gccactacgc gaacgtgaaa gcgacattat gttgatggca gaacactttg
    25201 ccatccagat gtgtcgggaa atcaagctgc ctctgttccc gggttttacc gagcacgcca
    25261 gagaaacatt gctgaattat cgctggccgg gaaatatacg tgaattgaaa aacgtggtgg
    25321 aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc
    25381 cctttaaacg ccgtccatct gaagaagcca tcgccgtttc agaaaacacc tcgcttccga
    25441 cgctgccgct ggatttacgc gagtttcaga tgcagcagga aaaagaactg cttcaggtca
    25501 gcttggaaca agggaagtat aaccagaagc gcgccgctga attgctgggg ttaacctatc
    25561 atcagttccg cgcgttgctg aaaaagcacc agctttagca tttcgacaat gcaacttgcc
    25621 gggtgtgccg tgaacgcctt atcaggccta caaaatcaat acgtcgtttg caggcctgat
    25681 aagcgctgcg catcaggcat tattgcgtta accctgcttc tgcgtattaa tctctcagcg
    25741 catcctgcaa aattttcatt gccgcatcga attgcgcaac cgggatggta tgggcttcgt
    25801 taggcggcgc atatttgtgg tatttgatcc gccgctatcg caaagtgcca ttgtatgatg
    25861 gcgacagaac gccggtgagc gaaacgtaat agtcgaatgc gcggcgcatt atcctgcctg
    25921 agggtaatgc accgcgccat taactataaa aatatgtatg acccggatca cgagtgctat
    25981 catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg
    26041 ctaaaatatt atgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac
    26101 gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat
    26161 gaagagatca gtgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
    26221 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc
    26281 agactatcgg tcagtatcgt tgcccggata aagcgttcgc gccgcatccg gctaaaaatg
    26341 acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
    26401 agttgttctg tgagttatag ttataaagaa atcacgggta cgaatgttga actgatggcg
    26461 acgccacctg ttcaatgacc aaccacacgg ccctcattat tacctctgaa cacaaacggt
    26521 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt
    26581 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
    26641 gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc
    26701 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
    26761 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgtctgatg
    26821 ccggaacttg ccgaaagctg ggaagtactc gacaacggcg caacctatcg cttccacctg
    26881 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
    26941 gacgatgtgg tttttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtt
    27001 aatggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc
    27061 cgcaaactgg ataatcatac cgttgagttc cgtctggctc agccggatgc ttcttttttg
    27121 tggcacctcg cgacccatta tgcttcggtc atgtcggcag aatatgcagg gaagttagag
    27181 aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg
    27241 tcggaatacc gcgccgggca atatattcgc ttacaacgtc atgatgattt ctggcgtggt
    27301 aagccgttga tgccacaagt ggtggtggat ttaggctctg gcggtacagg acgattgtcg
    27361 aaactcctga ccggggagtg cgacgttctg gcctggcctg ctgccagtca gctatccatt
    27421 ttgcgtgacg acccacgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg
    27481 gcattcaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg
    27541 gcaattaata accagcgcct gatgcaatcc atctattatg gtacggctga aacagcggcc
    27601 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat
    27661 ccggcaaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg
    27721 tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaaccgc cgaactgatt
    27781 caggcggata tggcacaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgtttt
    27841 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac
    27901 agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcgcag
    27961 accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
    28021 tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag cgcaaagtat tctggcgcag
    28081 gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
    28141 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
    28201 caggatgagg tgaaaaagcc atgattatct tcaccttacg ccgtattttg ttattgattg
    28261 tgaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcacc ccacatgcgc
    28321 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact
    28381 gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct
    28441 tcccggcaac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
    28501 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
    28561 tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt
    28621 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga
    28681 aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcaccg tggcgggatg
    28741 aaatgatcat tagcgcaatc cgccatatgg tattgcccgt gatcaccctg tcggttgcgc
    28801 caacaactga agtgatccga ctgatgcgta tcagcactat cgaagtgtat gaccagaact
    28861 atgttaaagc agcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
    28921 tgcataacgc gttacctccg gttattcctc gcctgggctt acagttttcc accatgctga
    28981 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
    29041 acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
    29101 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
    29161 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
    29221 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
    29281 gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag
    29341 caatttctcg gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc
    29401 ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg
    29461 ccaaccgtag gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg
    29521 ggcaccttcg ccggggcgac ccacggccta cgctcagcgg tgcttaacca tattctggat
    29581 accttactgg cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt tgccggaccg
    29641 agtttgtctc acgccatgtt tgctgtctgg ctggcgctgc tgccacgtat ggtacgttcg
    29701 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
    29761 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc ccaacatcac cgccgggctg
    29821 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
    29881 ctcgatctcg gcgcacaact gccctcgcct gaatggggag cgatgctcgg tgatgcgctg
    29941 gaactgattt atgtcgcccc gtggactgtc atgctgcctg gtgcggcgat tatgattagc
    30001 gtgctgttgg ttaacctcct tggcgacggt gtccgtcgtg caattattgc gggggtggaa
    30061 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
    30121 ttaaagccgt cgaccgcgta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
    30181 gcgaatccgg ttccggtaaa agtttgattg cgaaagcgat ttgtggggtg aataaagata
    30241 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
    30301 cacgcgaacg gcgcaagctg gttggtcaca acgtgtcgat gattttccag gaaccgcagt
    30361 cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
    30421 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
    30481 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
    30541 ccgaaggcga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
    30601 tgattgctga cgaaccgaca aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
    30661 tcctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
    30721 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acggtggaaa
    30781 ccgcgccgag taaggagttg gtgacgatgc cacaccatcc ttatacccag gcgctgatcc
    30841 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg
    30901 gcgctatccc gctactggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt
    30961 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
    31021 cctgtcattt cccactgaac atggagaaag agtgagatga tcgaaacgct acttgaagtg
    31081 cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa
    31141 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tatcggcgag
    31201 aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga acctaccagc
    31261 ggcgagttat tgattgacga tcatccgctg cattttggcg attattcctt ccgcagtcag
    31321 cgtattcgca tgatttttca ggatccttcc acctcgttga atccccgtca acgtatctcg
    31381 caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa
    31441 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
    31501 catatgttgg cacccgggca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc
    31561 ccaaaagtca ttattgccga tgaagccctc gcctcactgg atatgtcgat gcgttcgcag
    31621 ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc
    31681 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
    31741 gttgtcgagc gaggcagcac cgcagatgtg ctggcatcgc cgctgcatga actcaccaaa
    31801 cggctgatcg ccggtcattt tggcgaggca ttgacggcgg atgcgtggcg taaggaccgt
    31861 tagtgttttg cacctcatcc tgacaggccc ccctcaggat gaggtgttca ttacttttgg
    31921 ttgtcgattc tggtgagagc aaccagcaat gccagcgtat aacacaccgt aattgccaga
    31981 ctcattttta acagtgtttc tccgccaatt cccgacaatg gcgcggctat accgccgaag
    32041 acaaacatgc tcatccccat caacgcagac gccgttccag attcctgcgt ccctactgca
    32101 ctcatagcct ctgccccgcc aacagtgccg atgccgctac aaaatgcgat ggtgaaaaat
    32161 aatgccacca gtgccggaat gggcaattgt atccatgcgc ataagaccgt gagcaatgca
    32221 cacaaaattg ccgctatcag gccacctcgc aacaatgtca tcgcgttaat ccgtcgcgcc
    32281 aggcgcgaga agatccatga agcaatgata agtccggtgc cgttaaggcc aaacaccagg
    32341 ctaaattgca ttggactaaa gccaaattcc ttctgcaaca cgaacgacga agagccgata
    32401 tatgcaaaca aaccggccag cataaaactt tgaatcaggc aaaagcgcat aaaacggcgg
    32461 ttctgcacca cgcttcgtcc ggttaatagc aatgatgagc cccttttatt ttctggcaag
    32521 gtctcatgaa taaataacag acagcccagc aacagtacgg tgctaatttc agccattacc
    32581 cagaataaag tgcgccagtc aaaagtgcta acaatgtacc cgcccagcac tggcgacaac
    32641 accggtgcca ggccattcac cgtcattaac agcgcaaaaa actgggtcaa cgttactccc
    32701 tgatatttgt cacgagcaat agaacgagag agcaccgaac cacccgcccc cgcgatccct
    32761 tgaataaaac gccagaccac cagccagtaa atattgttcg ttgtcgcgca taaaatggaa
    32821 gaaacaataa acaatagcaa cgacaagatt aacggacgtt tacgccctat tttgtcgctc
    32881 agagggccaa ataacagttg tccgacgcca agaccaatca gtgatgcggt cagggttaat
    32941 tgcgttatgg tcgtggttgc cgccagttgc tcagacatct ccggcaaagc cggcaaataa
    33001 agatcggtac acatcgggcc gataccggct aaaagaccaa gaatcaatgc ccatgaaaga
    33061 gagactctag ccatattatt ccttacttga gtgatgacat accgccgccc agcgactgcc
    33121 agagcgtgat tcggtttgta aaatcagtca gttgcagtgc cagcagctcc tgctgtgcgc
    33181 tccacaagct gcgctgcgcc gtcaacacgg tcaggtaatc accaacaccc gcctgatagc
    33241 gacgcaagcc aacatcgacc gtttgttgtt cagctttaac atactggcgc tgtgcatcca
    33301 gttgctcttc aagcgtggtc cgccgcgcca gtgcgttagc gacatcttta aatgcgctct
    33361 gaacggtttt ttcataggtg gcaatgagtc cacgcttttg tgcttctgcg taacgaagct
    33421 gcgccagatt acttccgcca gtaaacagcg gtaatgtgac cgatggcgca aatgaccaga
    33481 tctgcattcc gtggctgaac aacgacgaca acgcgtcact gccaacaccg gcactggcag
    33541 tcagcgaaat cgtcgggaaa aagttagccc gcgccgcgcc aatatctgca ttggcgctct
    33601 tcaaattatg ttccgcttcc tggatatccg ggcgacgcag cagaacatca gatgatactc
    33661 ccgcaggaac cagacttatc atttgttccg gcaggctttc cagtgttccc ggtagcagat
    33721 tttccgcaag cgttgtacct gccagtaagt tcagcgcatt tttatcctgc atcacttgtg
    33781 tctgatagct ggcaacgctg gcgcgcgctt gctgataaac actcatcgcc tcactgacat
    33841 ctgtcgctgc cgccgtgcca acctgttgct ggcgctggat aattttcaat gagttttctg
    33901 cactggccat cgtttctttt gccagggcca ggttgctgtt atcagccgcc agtgtcagcc
    33961 aggcggtgct gatttcagca atcagcgtta aacgagtgtt ctgggctgta aattcactgg
    34021 caagccaggt ttcccgcgcc gcccgagata aactctgatt acgaccaaac agatctaacg
    34081 tataactgga caccgtgccg tcagcctcgg cagttgttcc cgtaccattc gccagcgaac
    34141 ggctgcgggt cgaacttaag gccgcattca ccgtcggaaa taacgacgca ttagtttgcc
    34201 catagagcgc ccgtgctgag tcaatatcag caatcgcttt ttgcacatcg cggttactgt
    34261 tcagtgcaat ggttactacc tgctgcaatc ggggatcgtg gatcacctgc tgccagtcat
    34321 ggctgattgc cttcccctgg ccctgtgctc ccggcaaggt cgccgggatt ggggattcag
    34381 gcgtactgta gtgaggatcg agcgagacac atcccgcact caacaacacc agaaaaataa
    34441 gagaacgacg caacataact tactcctgac ggcgcggttt accggcaaac aaacgtttca
    34501 ccagtacaaa aaacagagga acgaagaaaa tggccagtaa tgtagcggtc agcgtgccgc
    34561 caataatgcc cgtaccaatg gcgatgcggc tgttcgcccc tgcgccggtt gctatcgcca
    34621 gcggcattac ccccgcaata aacgccagcg aggtcatgat gattgggcgt aaacgagtct
    34681 gcgccgcgcg taatgccgca cgactcaagg aatagccctc tgcaaccgcc gcttcagcaa
    34741 attccacaat cagaatggcg tttttcgacg acaagccgat agtagttaat agcgctacct
    34801 ggaagtaaac gtcgttgttt aaatcacgca tccaggccgc cagcgccgcg ccaagaagcc
    34861 ccagagggat caccagaatg accgagaacg ggactgacca gctttcgtac agtgctgcaa
    34921 ggcagaggaa aaccaccaga atagaaactg catacagact taatgcctga ccgctggcca
    34981 gtttctcctg caatgacaaa ccactccagg cccaggttgt tcccgcagga agactgtttg
    35041 ccagttcctc catttttgtc attgcatcgc cggaactaaa gccagtcgcg ttttcgccct
    35101 gaatttcata ggctgccgag ccgttgtagc gcaccagtct ttccggtcca tacagccagc
    35161 gggtggtcgc aaaagcagag aatggtgtca tggcgttatc gctgccgcgc acaaaccatt
    35221 tacctaagtc tgacggcgcg gaacgaaatt cgctgtcgcc ctgaatgtag acttttttca
    35281 cacgaccacg gtcaataaag tcattcacat aagtaccgcc ccacgcactg gacagggtgt
    35341 cggtgacatc attcaaactc aaccctaatg acaccgcttt attactatca atatctacct
    35401 gcaattgcgg catttgtggc aaatcattgg cgcgtacaga atgtaactcc ggactttgat
    35461 tcgctttttc tatcaactga ttacgcattt gcagcagtgt ttcacgatcg gttccaccgt
    35521 tagctaacaa ttcaaacgta aaaccattgc tttggcccag cccatcaacg gctggcggcg
    35581 tcatcgcaaa taccgtggca tcacgaattg tgcccagctc tttggttgcc cgtagggcga
    35641 tagcctgggc ggtgttttct gcccctttac gttgagacca gtttttcaac gaaacaaacg
    35701 ccatcccggt gttctgtccg ctaccgctaa aactgaaacc atcaacggta aagatgacat
    35761 cggtatttgc tttctcgtta atcaggaacc agtcaacaat ctggcgattc acttctgctg
    35821 tacgggcagc ggtcgccccg gccggcagcg tgtactgcac catgatttcg ccctggtctt
    35881 cagtgggtaa gaaactgccc ggcagtttcc acatcatcag ggccattcca ccacccagca
    35941 cgacataaag ccccatcgtt cgggctgcac ggcgcaggac ataaattacg cctcgctgat
    36001 atttatcttc agtacggcgg taaaagcggt taaatgcgcc gaaaaagcct tttttatgtg
    36061 gtggaacatg ctggaggacg gaaccgcaca gggcgggagt caaggtcaat gccaccacca
    36121 cggaaagcag cattgcggag atgatggtga tggagaactg acgataaatt acccccgtag
    36181 atcccccaaa gaaggccatc ggcaggaata cggctgacaa caccagcgca atggcaacca
    36241 gtgcgccaga aatctcgccc attgattttt ccgtggcttc acgcgcgggc aacccttcgt
    36301 cacgcataat gcgttcgacg ttctctacca ccacaatggc atcgtccacc agcaagccga
    36361 tcgccagcac cattgcaaac aacgtcaggg tattaatcga atagccaaac aacgcaagaa
    36421 cgccgaatgt gcccagcaaa acgacgggga cagctaacgc cggaatcagt gtggcacgca
    36481 ggttttgcag gaataaatac atcacgcaaa ccaccaatac gatggcttca aacagcgtct
    36541 gaattacgtc ctctacggag attttaataa attcagtgct gtctttcggg tatgcgatgt
    36601 cgtatccctg cggcatgttc cgctggaatt cggcaatctt atccttgacc agcgtcgccg
    36661 tattcagcgc attcgcaccg ggcgaaagca ttacagcaac cccggcagac gggtgaccgt
    36721 taagtttgcc gatagcggta taatcttcac tgcccatctc aacgcgagcc acatctttta
    36781 tacgaacaac tgcgccgtct gactggcttt tcacgataat atttttgaac tgatccaccg
    36841 tctgcaagcg agactgcgca cgtaccgttg cggtcagttg ctgagtatcc ggtgaaggca
    36901 atgcccctat tttcccggca gtaacctgca cgttttgcgc ttcaatagca ctttgcacgt
    36961 ctgaaggcat cagcgagtaa gacgccagtt tggccgggtc aagccagatg cgcatagcgt
    37021 attccgcgcc aaagacttgc agactcccca caccttcaac acgcgccagc gggtcctgaa
    37081 cgttactgac cagccagtcg gcgatatccg aactggaggc tttgtcagtg gtgtcataca
    37141 cggcagcaat cagcaaaaag ttgctttgtg atttctccac cgtaacaccc gtttgctgca
    37201 cttcgctggg taggcgcgac tccgcctgct gaattttatt ctgcacctga acctgtgcag
    37261 tatctggatc ggtaccttgt tcaaaggtca cattaatact gaccgaacca tcagagctac
    37321 tggttgagct gaaatagagt aaattatcaa gcccagtgag ttgttgctcg ataacctgag
    37381 tcacgctgtt ctccagcgtt tcggcagaag caccagtata agtggctgaa attttaatgg
    37441 tcggcggtgc aacgtcagga tattgcgcga cgggcaatgt gcgaatagcc agaatccctg
    37501 ccaacataat caaaatggcg ataacccagg caaagaccgg gcggcgaaca aaaaagcgcg
    37561 aaaacatcag gcgttgcctc cattagcctg aacttcaaca gccttgacgg tctgccctga
    37621 agtgactttg gcagaacctt caacaatcag tcggtcgccg ttgtgcaggc cgttcagcac
    37681 cagccattta tcaccatacg tttctcccgt ttcgagcgtt cgctgctcta ctttattgtc
    37741 tttattcacc accagcgcag ttgcattgcc tttagcatcg cgcgtaacgc cctgttgagg
    37801 cgcgagaata gcgttttcca tcacgccttc atcgacacga gcgcgaacaa acattcccgg
    37861 taataactgc tgttgtggat tggggaaaat tgcccgtaat gtcaccgaac cggtagactc
    37921 atccaccgcg acttcggtga gttccagacg ccctttttcg ctgtaggttg tgccatcttc
    37981 cagaataagt gagacgctca tggtgtcact gttggtcgcc agtgactgtt tacgcagacg
    38041 taataaatcg acactggagc gagtgaggtc gacatacatt gtatccagac cacgaatagt
    38101 cgttaacgct gtatcctgcg acgcggtcac cagtgcgcca ggggttaccg acgaaatacc
    38161 aatacgcccc gaaatcggtg cggttaccgt ggtccagtca agattaatgc gcgcagtttc
    38221 cagtgcggct tttttcgcct ctacactggc tttatcttgt gcacaggtag actgagcatc
    38281 atcagcatcc tgttgtgaaa caccattctc tttcaccagt cgggcataac gctgcgcttt
    38341 ctggcaatcg gcttttacca gtgcctgtgc ttgttgtaat gctgcccggg cttcattcca
    38401 tgcagcctga taactggccg catcaatctg gtagagaggc tgtccagcct tgaccagatc
    38461 accttcctta aataagcgtt tctggataat tcccccaacc tgcggacgta cttcagcact
    38521 gagcgcagca ctggtgcgtc cggttaattc actgactacc gaaaccggtt gagatttgag
    38581 cgtaacaacg ccgacttctg tttctgacgg ggatgaattg ttactttgcg tattatcaca
    38641 acccgataag agcagcattg ccactacaga tgttgctata tacttcatta ttttctccag
    38701 ggtgagaaat accctcctca ttgaaattaa tgaggatatg tcagggatat tcagttataa
    38761 cgaacccgca tcgcgaaatg aatttcgcaa tgaaataagg gggattaata tcccccgcta
    38821 ttggttaatt ctgttcttat ttatttatca acatactaac aatatcctga tatatttcct
    38881 gcagatgttg tggatcgctt ttttgcggtg ttaaacgacg ataaaccgta ccttccatca
    38941 tggtagcaaa cacttcaacg cagcaacgaa tgtgctcgtc gctaagatgg ggatgcattc
    39001 ttttcatatg tgcacaaccg ttagcgaaca ttctggcgtc agcttcttca agcattgcca
    39061 taacccgcgg gttgcgtgtg gcttctgcag ccacttccag catcagcgcg tcgtcgtcct
    39121 catttaagga tttacgcaag gcaaggactt ccgggaagtg atcggtacgc gcgtcaatgt
    39181 ccatttgggc aatacgaaag tcgatgatac ggcgaaccat ctcttcaata atggcgtctt
    39241 tgttggcgaa gtaacgatat atctgaccca cgctaagctg agcttcgctg gcaatttgcg
    39301 acatactggc ggcatgaaat ccgcagcgac gaaaacaccg acgggcggcg ttaatgattt
    39361 catcctgacg ctgtttgtgg cgtatctcca gcttgctggt catttctgtc tcctggccta
    39421 tcacttggtg agaatgatca ttctcatttt tgctaattat acgtttctcc aaagtaatgg
    39481 aaagtatttt tatcttattc atgatgcata ttctgataat gaagagattg tggagattct
    39541 gtgaagatag ttgacaatat taagttggag tcaattcatc agaaagacag cgacttgttg
    39601 tcgccagttc tgttttcatc attaaactta taggagctaa tacgaatagt ttcaaagtca
    39661 gattctataa gatatttccc caacctcccc cttgcaggcg tcaggagtag ataacgcaaa
    39721 atgctctgta ccgcagcttc tctccgctac agaaagcgca actatagcca cccacagcca
    39781 ggttgattat aataaccgtt tatcagttcg tactgtttac taaaacgacg aatcgcctga
    39841 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
    39901 gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta tcgctcaggc
    39961 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
    40021 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgccga
    40081 agataccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
    40141 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
    40201 cgttactcgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
    40261 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
    40321 tggcgctgag cgcgctatcc cgaattacaa cgttatgggt ctggcaaaag cgtctctgga
    40381 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
    40441 ctctgctggt ccgatccgta ctctggcggc ttccggtatc aaagacttcc gcaaaatgct
    40501 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
    40561 ctccgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
    40621 tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ctgaaataat cgttctgttg
    40681 gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt atacctttct gatatttgtt
    40741 atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt tcctttacga
    40801 ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt atcatgaaac
    40861 gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg caaaggtcag
    40921 cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggccccct tcctttcatg
    40981 gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gacccgctta ccgtgactcg
    41041 ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg ctcaggtttg
    41101 cggcgtccaa cggttatgta actactattc tgcgcgactt acgccgctcc ccgggcctga
    41161 ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc gccaactggc
    41221 tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg ttggtcttac
    41281 tgtctgtgac tgtgtgatca ttaaccaaat cattggtttt attggctttc aggcgcggac
    41341 cattgcgaca tttcaggctt atctcgggca tccagtacgc tggttacccg ggctggagat
    41401 acaaaactac gccgacgcgt cactgtttgc tgatgaatca ttacgctggc gaagcagcta
    41461 tgaggtggaa aaactccctg aagagcacac aaaaagttca actgcagaac tttgccaact
    41521 ggccaacaca ctctctctcc accctatttc actttccctt ctcgaaaagt tgttaaacag
    41581 cacacgggtc aatacacagc cggataatca gcttgcggcg ttgttatgcg cgcggataaa
    41641 tggcagtcct gcttgttttg ccgcctgtat gaattcatca aatgaatata aaaaaatcag
    41701 cccccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc attctgttga
    41761 gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct ttagcgccgc
    41821 ccagttcagc ccattactcg aacacgaaaa atcatcaacg cagattatta atctgctagt
    41881 atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg agacatatta
    41941 accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat
    42001 agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa ctgcattccc
    42061 agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc ttcctggaag
    42121 tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg
    42181 accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag ccagaagaac
    42241 tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg
    42301 ccatcgttcc tgaccatcca ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc
    42361 tgaaccacga gtttaaagaa ggtgactggg cggttgccga aatgcgccgt catccgctga
    42421 aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt gacgatcact
    42481 ttgtaccgtg gtgggttacc cttgcgcgtc ataatctgga aaaagaagca ccagacggcg
    42541 ttgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgactgcg ctggattttg
    42601 tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgcc aaggcgttac
    42661 cggatggcaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg attgctgaag
    42721 gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg cctggcttca
    42781 acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc gccaatgaag
    42841 tccgcccggt actggcatgc cgcatgacgc tctccactga tggcaccatt gaagataata
    42901 tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac caggtttctg
    42961 actggctgga aaataccggt gactggcagc ctgaaagcga agcaattgcc gaacaagtcc
    43021 gtttgctagc acaaatttgc caacgccgcg gcgagtggcg tcataaccac gcactggtgt
    43081 ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg
    43141 tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca
    43201 tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac gtgcatatgg
    43261 gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg
    43321 tcgatgctga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt gaactggatg
    43381 cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt gctgaaatta
    43441 gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccact tggacttcgc
    43501 cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt atcaaaggcg
    43561 aaactgcgac gcgtccacag gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca
    43621 accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg aaagacaaag
    43681 ccgggaccga cacccgtttc gcagcggaaa ttgtcgatat cagccgtggc ggcatgcgtg
    43741 ttcgtttggt tgataacggc gctatcgcct ttattccagc acctttctta cacgctgtgc
    43801 gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt gaaacggctt
    43861 acaaagtgac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa acccgcagca
    43921 ttattgcgcg cccagtcgcg taatctcctt tcacggccca ttcctcatgg atgggccgtt
    43981 tatttccccg ctcaaccttc gtcatatccc ggcagatttt taactgactt tcgtttgaaa
    44041 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat gtgtttagcc
    44101 gccgttaatt tcatgtaagt catgttgttc cttttattta actgcggact ccgctgttaa
    44161 ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca actttctcgg
    44221 atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct tttctaccac
    44281 cgaaatcaca gaacccgatc gtattttgca actatctgcc gaacaggctg ctcgcatcag
    44341 ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg aaagcgatct
    44401 ttctgtgcat ctggtaggac gaaaaatcaa taaacgagaa tggggaggca acgcgtctgc
    44461 atggcatgac acaccggcgg ttgctcgtga tttatcacat gggctttcct ttgctgagca
    44521 ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga atatccagcg
    44581 ctttaatcgg ttatgtgaag attacacagg cttgaaagaa cacgacgtca ttgggcaaag
    44641 cgtgtttaaa ctgtttatga gtcgtcgtga agctgcggca tccaggcgca ataaccgtgt
    44701 attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac gtaaaggcca
    44761 gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa acgagatttt
    44821 tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggagc gactgcgtat
    44881 tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc aggagttaat
    44941 cgatcacgct attaatcagg cagataacaa taaagttggg gttgtgtatc ttgatttgga
    45001 taattttaaa aaggtcaacg acgcctatgg gcatttgttt ggtgaccagt tattacgcga
    45061 cgtgtcattg gctattttaa gctgtctcga acatgaccag gtattggcgc gtccaggtgg
    45121 cgatgagttt ctggtactgg catccaatac ctcacaaagc gcgctggaag caatggcatc
    45181 acgaattttg acccgcttac ggctcccctt tcgcattggt ttaattgaag tttataccag
    45241 ctgttcagta ggtatttcac tctctcccga acatggttcg gacagcgcgg ctattattcg
    45301 tcacgccgac acagcaatgt acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt
    45361 taccccagaa atgaatcaga gggtatttga atatctctgg ctggatacca acttgcgtaa
    45421 agcactggaa aacgatcagt tggttattca ctatcaaccg aaaatcacct ggcgtggtga
    45481 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    45541 gttggacttc atttcctacg ccgaagaatc agggctaatt gtgcctttag gccgttgggt
    45601 gattctcgat gtcgtacgcc aggtagcgaa gtggcgggat aaaggcatta acctgcgagt
    45661 agcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    45721 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cggagagctg
    45781 cctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc gattaggtgc
    45841 gcaagttcat ctggacgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    45901 tccgatcgat gccatcaaac ttgaccaggt ttttgttcgg gatattcaca aacagcctgt
    45961 ctcgcaatca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    46021 cgccgaaggt gtagaaagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    46081 gcgacaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    46141 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat attccgtcat
    46201 tatatatcgt attcccggat gataattagc cgttgctgtt aaacccttct cttttagcgg
    46261 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    46321 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    46381 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    46441 tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt gccttcaggt
    46501 tttcctattt gaggatgccg gcgcgtcagt atttttttat ttagtattat aacgttataa
    46561 gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt gaccagggtc
    46621 aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc attcgccagg
    46681 atctcaacac cctcgaaaaa ctgagttacc tccgacgtgc acatggtttt gcagtttcgc
    46741 ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag cgtgaactcg
    46801 ccgagtttgc cgcgtcactg gttcaaccgg gcgaaaccat ctttatcgaa aatggtagca
    46861 gcaatgccct gctcgcccgg actctgggcg agcagaagaa aaatgtcact atcatcacgg
    46921 tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg
    46981 gcgtgtacca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag tgcatccaac
    47041 aggtgcattt cagcaaagca tttattggta ttgatggctg gcaacctgaa actgggttta
    47101 ccggtcgcga tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa gagtgcgagg
    47161 cgatagtcct gaccgacagc tcgaaatttg gtgctgtaca ttcatactcc atcggccccg
    47221 ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat ctgatgcatc
    47281 ttgaacaaag taaactcacc gttcacgtcg ttgacattta accatttccc ttctctacgg
    47341 atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat aattaagact
    47401 atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat aacaggaagt
    47461 cattatcacc tgcgtgatat aaccctgcgc gcgagcggat ttcacggaat aatttcacct
    47521 ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat aaattcagga
    47581 gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc aattaccctg
    47641 gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac cgcaatcggc
    47701 gcaggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt gggtacatta
    47761 ggtggtgcag ccgtcggtgg tgttattggt caccaggtcg gtaaataatc gtaaataaat
    47821 gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa ataactgaat
    47881 aatacacaca gtaaaatatt c
//
LOCUS       DAPQZD010000015        47324 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPQZD010000015 DAPQZD010000000
VERSION     DAPQZD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.015"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..441
                     /locus_tag="RO454_000875"
     CDS             <1..441
                     /locus_tag="RO454_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0932748.1"
                     /translation="RQKEKALDFSDLPPQKAIEKIIDFTWDYYISNPWFLKIVHSENQ
                     SKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNINIAALGGYYLI
                     NQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(425..1063)
                     /gene="ytfB"
                     /locus_tag="RO454_000876"
     CDS             complement(425..1063)
                     /gene="ytfB"
                     /locus_tag="RO454_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDY0932749.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     ESQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1282..1902
                     /gene="fklB"
                     /locus_tag="RO454_000877"
     CDS             1282..1902
                     /gene="fklB"
                     /locus_tag="RO454_000877"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDY0932750.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2211..3623
                     /gene="cycA"
                     /locus_tag="RO454_000878"
                     /gene_synonym="dagA"
     CDS             2211..3623
                     /gene="cycA"
                     /locus_tag="RO454_000878"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDY0932751.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3668..4330)
                     /gene="ytfE"
                     /locus_tag="RO454_000879"
     CDS             complement(3668..4330)
                     /gene="ytfE"
                     /locus_tag="RO454_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDY0932752.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4438..5403)
                     /locus_tag="RO454_000880"
     CDS             complement(4438..5403)
                     /locus_tag="RO454_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDY0932753.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5511..6371)
                     /gene="qorB"
                     /locus_tag="RO454_000881"
     CDS             complement(5511..6371)
                     /gene="qorB"
                     /locus_tag="RO454_000881"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDY0932754.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETDKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6460..6840
                     /locus_tag="RO454_000882"
     CDS             6460..6840
                     /locus_tag="RO454_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDY0932755.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6969..8912)
                     /gene="cpdB"
                     /locus_tag="RO454_000883"
     CDS             complement(6969..8912)
                     /gene="cpdB"
                     /locus_tag="RO454_000883"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDY0932756.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9102..9842
                     /gene="cysQ"
                     /locus_tag="RO454_000884"
     CDS             9102..9842
                     /gene="cysQ"
                     /locus_tag="RO454_000884"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDY0932757.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9832..10389)
                     /locus_tag="RO454_000885"
     CDS             complement(9832..10389)
                     /locus_tag="RO454_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDY0932758.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10714..10920
                     /locus_tag="RO454_000886"
     CDS             10714..10920
                     /locus_tag="RO454_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDY0932759.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10982..12325)
                     /locus_tag="RO454_000887"
     CDS             complement(10982..12325)
                     /locus_tag="RO454_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDY0932760.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12648..13286)
                     /gene="msrA"
                     /locus_tag="RO454_000888"
     CDS             complement(12648..13286)
                     /gene="msrA"
                     /locus_tag="RO454_000888"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDY0932761.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13492..15225
                     /gene="tamA"
                     /locus_tag="RO454_000889"
     CDS             13492..15225
                     /gene="tamA"
                     /locus_tag="RO454_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDY0932762.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15222..19001
                     /gene="tamB"
                     /locus_tag="RO454_000890"
     CDS             15222..19001
                     /gene="tamB"
                     /locus_tag="RO454_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDY0932763.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            19004..19345
                     /locus_tag="RO454_000891"
     CDS             19004..19345
                     /locus_tag="RO454_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDY0932764.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            19557..19808
                     /gene="chpS"
                     /locus_tag="RO454_000892"
     CDS             19557..19808
                     /gene="chpS"
                     /locus_tag="RO454_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDY0932765.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            19802..20152
                     /gene="chpB"
                     /locus_tag="RO454_000893"
     CDS             19802..20152
                     /gene="chpB"
                     /locus_tag="RO454_000893"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDY0932766.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(20232..20762)
                     /gene="ppa"
                     /locus_tag="RO454_000894"
     CDS             complement(20232..20762)
                     /gene="ppa"
                     /locus_tag="RO454_000894"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDY0932767.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            21072..22028
                     /gene="ytfQ"
                     /locus_tag="RO454_000895"
     CDS             21072..22028
                     /gene="ytfQ"
                     /locus_tag="RO454_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDY0932768.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22168..23670
                     /locus_tag="RO454_000896"
     CDS             22168..23670
                     /locus_tag="RO454_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDY0932769.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            23684..24706
                     /locus_tag="RO454_000897"
     CDS             23684..24706
                     /locus_tag="RO454_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932770.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            24693..25688
                     /gene="yjfF"
                     /locus_tag="RO454_000898"
     CDS             24693..25688
                     /gene="yjfF"
                     /locus_tag="RO454_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDY0932771.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(25721..26719)
                     /gene="fbp"
                     /locus_tag="RO454_000899"
     CDS             complement(25721..26719)
                     /gene="fbp"
                     /locus_tag="RO454_000899"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDY0932772.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            26895..28268
                     /gene="mpl"
                     /locus_tag="RO454_000900"
     CDS             26895..28268
                     /gene="mpl"
                     /locus_tag="RO454_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDY0932773.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(28419..28970)
                     /locus_tag="RO454_000901"
     CDS             complement(28419..28970)
                     /locus_tag="RO454_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDY0932774.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            29064..30416
                     /gene="pmbA"
                     /locus_tag="RO454_000902"
     CDS             29064..30416
                     /gene="pmbA"
                     /locus_tag="RO454_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDY0932775.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            30599..30985
                     /gene="cybC"
                     /locus_tag="RO454_000903"
     CDS             30599..30985
                     /gene="cybC"
                     /locus_tag="RO454_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDY0932776.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(31030..31494)
                     /gene="nrdG"
                     /locus_tag="RO454_000904"
     CDS             complement(31030..31494)
                     /gene="nrdG"
                     /locus_tag="RO454_000904"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDY0932777.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(31653..33791)
                     /gene="nrdD"
                     /locus_tag="RO454_000905"
     CDS             complement(31653..33791)
                     /gene="nrdD"
                     /locus_tag="RO454_000905"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDY0932778.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(34185..35840)
                     /gene="treC"
                     /locus_tag="RO454_000906"
     CDS             complement(34185..35840)
                     /gene="treC"
                     /locus_tag="RO454_000906"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDY0932779.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(35890..37311)
                     /gene="treB"
                     /locus_tag="RO454_000907"
     CDS             complement(35890..37311)
                     /gene="treB"
                     /locus_tag="RO454_000907"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDY0932780.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGNFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(37430..38377)
                     /gene="treR"
                     /locus_tag="RO454_000908"
     CDS             complement(37430..38377)
                     /gene="treR"
                     /locus_tag="RO454_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDY0932781.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            38562..38615
                     /gene="mgtL"
                     /locus_tag="RO454_000909"
     CDS             38562..38615
                     /gene="mgtL"
                     /locus_tag="RO454_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDY0932782.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            38756..41452
                     /gene="mgtA"
                     /locus_tag="RO454_000910"
     CDS             38756..41452
                     /gene="mgtA"
                     /locus_tag="RO454_000910"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDY0932783.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(41658..42044)
                     /gene="ridA"
                     /locus_tag="RO454_000911"
     CDS             complement(41658..42044)
                     /gene="ridA"
                     /locus_tag="RO454_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDY0932784.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(42117..42578)
                     /gene="pyrI"
                     /locus_tag="RO454_000912"
     CDS             complement(42117..42578)
                     /gene="pyrI"
                     /locus_tag="RO454_000912"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDY0932785.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(42591..43526)
                     /gene="pyrB"
                     /locus_tag="RO454_000913"
     CDS             complement(42591..43526)
                     /gene="pyrB"
                     /locus_tag="RO454_000913"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDY0932786.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(43530..43664)
                     /gene="pyrL"
                     /locus_tag="RO454_000914"
     CDS             complement(43530..43664)
                     /gene="pyrL"
                     /locus_tag="RO454_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDY0932787.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(43945..44340)
                     /locus_tag="RO454_000915"
     CDS             complement(43945..44340)
                     /locus_tag="RO454_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDY0932788.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(44471..45184)
                     /gene="bdcA"
                     /locus_tag="RO454_000916"
     CDS             complement(44471..45184)
                     /gene="bdcA"
                     /locus_tag="RO454_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0932789.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            45255..45848
                     /locus_tag="RO454_000917"
     CDS             45255..45848
                     /locus_tag="RO454_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0932790.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            45993..46445
                     /gene="tabA"
                     /locus_tag="RO454_000918"
     CDS             45993..46445
                     /gene="tabA"
                     /locus_tag="RO454_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDY0932791.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            46568..47137
                     /locus_tag="RO454_000919"
                     /pseudo
     CDS             46568..47137
                     /locus_tag="RO454_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11288 a        11820 c        12631 g        11585 t
ORIGIN      
        1 cgacaaaaag aaaaggccct tgatttctca gatcttccgc cacagaaagc cattgagaaa
       61 ataatcgatt ttacatggga ttattatatt tctaatccct ggtttttaaa aattgtgcat
      121 agtgaaaatc aaagtaaggg tgtgcattat gcaaaatcac agcgtctttt agaaattaac
      181 catgcgcact tgcagttaat ggaatcctta ttggatgaag ggaagaaaca aaatattttt
      241 aagccagata tcgacccatt gcaggtgaat attaatattg ctgcgcttgg cggatattat
      301 ttgatcaacc agcatacgct tggcctggtt tatcatatca gtatggtttc cccccaggca
      361 ctggaagcca gacgtaaggt catcaaagag acaatcctta gctggctttt ggttgaccct
      421 tcatctaccg cgcacgaata aaactgccat ccggctggcg ggtgaacagg acctgttgat
      481 tattccccgt atcaatggtt aagcccgtca ccacgccgct ggcgttctgg cggattttca
      541 ccatttgccc gttttgcaaa ttactcagcg gcttacccgc gccttctacc tgcgccattg
      601 catagacatc ggtagcgggc agtccgtggt cgcggaacag ctgcgccatc gttttaccgg
      661 gttcgacacg gtaagagcgc cattgattat caattccgct atctggctgg aatggttgtg
      721 tttgggtagt ttgcggctgt tcttctggct ggctttcctg tataggttct ggagcgaccg
      781 gagccacctg atcaggatca ttttgtggtg tgactaattg cgcccgcagc tgttcttctg
      841 tcggcggttg tgattgcgac tgaatatcta actgcgcttc acgcgtcacc accggcgcgt
      901 tgggcgtatc atcagatgga agcagaaatc cgaccaccaa tataatggcg gcaataatga
      961 tcccccgacg atgcatcggc ggcagcgggt ccatgaagcg aaaattgtcc ggcgcgtgcc
     1021 agactttctc cagggttggt tttagttcaa agcgcccggg catggtttcc tcctgctccg
     1081 cgtcttgttc ctcaatcata gcctatgaat aagctaacgc tatgatgtcc gtggtaaacc
     1141 cgcctttatt atattcatac gggatattgc tattgtttct ttttccccgg gatttgtcat
     1201 cattcctgcg acaaagtttt acccaaagaa gtgtggctga tatgctgccc gctactttta
     1261 taccctaaga aaggaaatac gatgaccacc ccaacttttg acaccatcga agcgcaagca
     1321 agctacggca ttggtttgca ggtagggcaa caactgagtg aatctggcct ggaagggctg
     1381 ctgccagaag cactggttgc aggtattgcc gatgcgctgg aaggcaaaca tccggctgtt
     1441 ccggttgatg tggtgcatcg cgcgctgcgt gaaatccacg agcgcgccga tgccgttcgt
     1501 cgtcagcgtt tccaggcgat ggctgctgaa ggtgtgaaat acctggaaga aaacgccaaa
     1561 aaagaaggtg tgaatagcac cgaatctggc ctgcaattcc gcgtgatcaa ccagggtgaa
     1621 ggcgcaattc cggcacgtac cgaccgcgtt cgtgttcatt acaccggtaa actgatcgac
     1681 ggcaccgtgt ttgacagctc cgttgctcgt ggtgaacccg ctgaatttcc ggttaatggc
     1741 gtgatccctg gctggattga agcactgact ctgatgccgg taggttctaa atgggaactg
     1801 actatcccgc aggaactggc atatggcgag cgcggcgcag gcgcatccat ccctccgttc
     1861 agcaccctgg tgtttgaagt cgaactgctg gaaatcctct aagcagcgca ttctgttccc
     1921 ctcgaaccag aggggagcag gcattcagca ataaaccctt cagtttgcca aacggcgcta
     1981 ttttgtgttg caaagacccc gtaagcgtgt atttttgtga gctgtttcgc gttatcaccg
     2041 tgatatgaca ctcactttaa acataaaatt aacattagat ctaaatctta gtattcatcc
     2101 cgcgtattgt tacctaatat cgatgagtcc cgatacagat tcgtcgtatc atagactgac
     2161 taaaggccgt agagcctgaa caacacagac aggtacagga agaaaaaaac atggtagatc
     2221 aggtaaaagt cgttgccgat gatcaggctc cggctgaaca gtcgctacgg cgcaatctca
     2281 caaaccgaca tattcagctt attgccattg gcggtgccat tggtacgggg ttgtttatgg
     2341 ggtctggcaa aacgattagc cttgccgggc cgtcgatcat tttcgtttat atgatcatcg
     2401 gttttatgct ctttttcgtg atgcgggcaa tgggggaatt gctgctttcg aatctggaat
     2461 acaaatcttt tagtgacttc gcttccgatt tactcgggcc gtgggcagga tatttcaccg
     2521 gctggactta ctggttctgc tgggttgtaa ccggtatggc agacgtggtg gcgatcactg
     2581 cttatgctca gttctggttc cccgatctct ccgactgggt cgcctcgctg gcggtgatag
     2641 tgctgctgct gacgctcaat ctcgccaccg tgaaaatgtt cggtgagatg gagttctggt
     2701 ttgcgatgat caaaatcgtc gccattgtgt cgctgattgt cgtcggcctg gttatggtgg
     2761 cgatgcactt tcagtcaccg actggtgtgg aagcgtcatt cgcgcatttg tggaatgatg
     2821 gcggctggtt cccgaaaggt ttaagtggct tctttgccgg attccagata gcggttttcg
     2881 ctttcgtagg gattgagctg gtaggtacaa ccgctgcgga aaccaaagat ccggagaaat
     2941 cactgccgcg tgcaattaac tctattccga tccgtatcat tatgttctac gtcttcgcgc
     3001 tgattgtaat tatgtccgtg acgccgtgga gttcggtagt cccggagaaa agcccgtttg
     3061 ttgaactgtt cgtgttggta gggctgcctg ctgccgcaag cgtgatcaac tttgtggtgc
     3121 tgacctctgc ggcgtcttcc gctaacagcg gcgtcttctc taccagccgt atgctgtttg
     3181 gtctggcgca ggaaggtgta gcaccgaaag cgttcgctaa actttctaag cgcgcagtac
     3241 ccgcgaaagg gttgaccttc tcgtgtatct gtctgctcgg cggcgtggtg atgttgtatg
     3301 tgaatcctag cgtgattggc gcgttcacga tgattacaac cgtttccgcg attctgttta
     3361 tgttcgtctg gacaattatc ctttgctcgt acctggttta ccgcaaacag cgtcctcatc
     3421 tgcatgagaa gtcgatctac aagatgccgc tcggcaagct gatgtgctgg gtatgtatgg
     3481 cgttctttgt gttcgtggtc gtgttgctga cactggaaga tgacactcgc caggcgctgt
     3541 tggtcacccc gctgtggttt atcgcactgg ggctgggctg gttgtttatt ggtaagaagc
     3601 gggctgctga actgcggaaa taaccgcatt atcatgctgg atggcgcaat gccatccagc
     3661 ttttagatta ctcacccgcc agcgcgcgtg ggaacagtac attgttttcc aggctgatgt
     3721 gatccatcag gtcatcaatc agttcattaa taccgttata catcgctttc caggtggtgc
     3781 aggcttccgg cggcggtgtg acgttattgg tggtgtgttt aatcacttcc agcagttcgc
     3841 ccgcttcatc gtgctcgctt tccatcacgc tgattggtcc cattgcctgg ctgcccatgc
     3901 cttgtttgat catcgggaag aggatctgct cttctttcat catgtggctg gaaagctctt
     3961 catgcagcat agtcaggtat tttgtcagcc cttttggcac gctcggtttg tcggcgtgaa
     4021 cgcgctcgac tttagtcgct tgcaggatca gctccggcag ctgctcgcgg tgacgatcgt
     4081 ggtagcgcac gatgatatgg tcaatgattt ctgccagcgg ggcgctacgc cagtctttct
     4141 caatcggttg ttcagcaagc tttgccagtt cagcttcaat gacctcaaca tccagttctt
     4201 tacgtgccgc cgcgcgcgcc agcgtctgct taccgccaca gcagtaatcc atatcatatt
     4261 tacgaaacag agccgaagcg cgagggatag agagcgccag ttcacctaaa ggttggtcgc
     4321 gataagccat agctgatacc tcattcttaa taatataaga tgtattttaa atgcatcttt
     4381 aaggcaaaag ctataaccct tacgtagcag aggcttattt aactcactgc cagcgtgtca
     4441 actttctgag acagattcag ttaaaggttt ttctggtttt gctctgaccg caatgaccac
     4501 gccaatcact aacagcgcga taccgctcag cgtcattagc ggcggcattt gctggcggag
     4561 taaaaaggtg tacagcaaac ctgccagcgt ttcgaaaaca atcagcggcc cgagaatcac
     4621 tgtcggtaat cgctggctgg cgacgttcca gcagagtgcg ccaacccatg agcaaagcac
     4681 ggctatcgca accatcagac taataaacac cagcggacgg gggccaaaag gtaaggagaa
     4741 gtccggcgtt tgtatattca gccagtaaca ggcgacgaga tagccgatga gagaaaccgg
     4801 cagcgtgacc agcgcctgcg ccgtcgccca catcatcgga tgtttgtcgg gattttcccg
     4861 cagccagcgg gcgttgcgca gggcatacca tgcccagcag accacggaaa ctaacgctag
     4921 cacgatgcca gaggtataac gtgcccagtc aaaatcgggg agtccgtggt ttaactcagc
     4981 aatattcaca ctcgccaggc cgatgccaat acaaatcagt gccggggcga gttttcccca
     5041 cgcgagtttg ccgtcgcgct ggctataaag cagattggca aagacaggaa tgaccaccgg
     5101 cagggtgccg ataatcatcg tggaaacagg cgcgccagta cgttgaatgg cactggcaag
     5161 gcagaagtaa taaatgaggt tgcccatcat agtgagcatc aaggcggtaa gccagtcccg
     5221 acgcgccaac tgacgcagac gcacgcgtcc cagccaggca atgggcagcg caattaaccc
     5281 taacgccaga taacgcccca tcgactgcaa catcgccggg tattccggta cgatcaacgg
     5341 cccgacaaaa ataagccccc acatcaaccc tgctaacagg gcgtacagca cgccgctaat
     5401 cattactggc atccattggt ctgtcagaag aaagttcagg aagcagacat gattgctccc
     5461 ggcatcccgt cattattaga gaggatgcgg gaggatgcca ctttaattaa ctagttatta
     5521 acattaaaaa gatggcttac gctttcggct aacgttgtcg ttgggcggcc aatcaatttg
     5581 ctaagcgttt tgctgttatc aaacagacca cctttcgatg cgccaacgtc agaatccgcc
     5641 agcatatccg ccagtccgtc gggcagtccg acgcttttca gtgccgcggc gaaatcggct
     5701 tcgctcagat tttgataggt aacggatttt ccgctctgtt tggtcagctc tgccgctaac
     5761 tgtgtcaacg tccaggcact atcgcccgcc agttcgtaaa ccttgccttc gtgaccggct
     5821 tcgctaatca cgcgtgccgc agctgccgca taatctgccc gcgttgctga ggcgattttg
     5881 ccatcgcccg ccgcaccgat aaatacgccg tgttccagtg ctgccggggc gctggcgagg
     5941 tagttttcgg tgtaccagcc gttgcgcagc agggtgtaaa cgatgccaga atcagccagc
     6001 attttatccg tctcgatgtg ctcatcggcg aggccgagcg gggaggtatc tgcatgtagc
     6061 aggctggtat aagcgataaa tttcacgcca gccgcctttg cggcattaat aacattacga
     6121 tgctgcgggg cacgttgacc cacttcgctg gaagagatca gcagtagttt ttccactccc
     6181 tgaagtgcag atgtcagtgc ggcttcatcg ccgtagtcag cctgacgcac ggtaatgcct
     6241 tgtgctgcca gggcctgggc ttttgccgga ttacgaacga tagccactat ttggctggca
     6301 ggaaccgttt tcatcaagga ttcaataaca tagtgaccaa gttggccagt ggcaccagta
     6361 atagcgatca tgggaagtct ccatcgtttt gtcttgtgtt taaatacgct aacacctaaa
     6421 cttactttta gtaagtacgt acaaaaaggt aagtatgaaa tgagtcaggt tagcctgtcg
     6481 caacaactga aagagggtaa tctctttgcg gaacagtgcc cgtcgcgcga ggtgttgaaa
     6541 cacgtcacca gccgttgggg ggtattgatt ctggtggcgc tacgcgaagg tactcatcgc
     6601 tttagcgacc tgcggcgcaa aatcggtggg gtgagtgaaa agatgcttgc gcagtcgtta
     6661 caggcgttag aacaggatgg ttttcttaac cgtatcgcgt atccggtggt gccgccgcat
     6721 gtggaataca gcctcacgcc gctgggcgag caggtgagcg aaaaggttgc tgcactggcg
     6781 gactggattg agttgaattt gcccgaggtg ttggcggtgc gggatgaacg tgcggcataa
     6841 ctgccggatg cgctgagctt atctggtcta cggtgcatgg cttgtagggc ggataagatg
     6901 cgccagcatc gcatccgcca ataaatgccg gatgcggttg atgatttata ggccgaaaaa
     6961 gaagtgtttt acttactcaa atccacctga taaatcgcaa acccgatatc atcggtcgcg
     7021 actttattca tcggatattg ccctttctct ttaataaacg ctgcggcttt atctgacggg
     7081 gaagtttcga aacggatatc cagtttctta tcgccagcaa tcggtgctaa acgccagttg
     7141 ttatctgccg ccgggtgaat ttcccccgca cgtttcgact catcagcaat ccacgctgcc
     7201 agcaccgagc ggttctcatc cggtgaagca aaagcgatat ggctgtcgcc cgtaccggca
     7261 aatttgccgc cgtaagcgcg atagttattg gtggcaacga ggaacatggc gttcggatca
     7321 atcggcttgc cattaaaggt caggttctta atcctttccg cattggcatt aatcatctgg
     7381 cactcgccgt cataacgggc aggctgggta acatcaatct gataattcac accatcaatc
     7441 acatcaaagt tataggtgcg gaaaccatcc cagttgatga gtgattgcgg tttcgtgctg
     7501 ttaggatcaa tctggttaaa ctgtccggcg gagcactcca gccactcttt cacctcttta
     7561 ccgctggctt tcactacaat cagcgtattg gggtagagat aaagatcggc ggcattacgg
     7621 aaggtcaact ggcctttttc cacctccaca tagcttgccg ggtcattttt gcgaccacca
     7681 actttaaacg gtgcggcagc tgaaagcacc ggcagttttg ccagatccgg atcgccctga
     7741 atgtaatgtt cgacatacgc tttttgcgcg ttgttcacta cttgcacggt cggatcgtcc
     7801 tgcaccagcg ccagatagct atacatattg tcggcagatt taccgattgg cttgctgacg
     7861 aactggcgtg tggcatcgtg atcggctttg agtgtttcta ccagcttgct gtcttccgcc
     7921 gcgagggatt ttttattggc gatgtcgtaa atcggccgag cttccgcttt cgcctgcgtc
     7981 acctgccatt taccgctgtc attactgagt tgtaagtcga ccacaccaag atgatcgccc
     8041 cacatgcctg gcattaccgc cggaacacca ttcagcgtgc ctttcgcgat atcagctcct
     8101 tcgatatcag caaaatcttt acttgggaaa acggcgtgag catggccaaa cataatggcg
     8161 tcaacacccg gaatttcact gagataataa actgagtttt ccgccatcac tttatacgga
     8221 tcggcagaca gcccggaatg cgccagaacg acaacgagat cggcaccttt ctcgcgcatt
     8281 tcaggcacgt atttgcgcac ggtttcggta atatcattca ccgtcacttt cccggataaa
     8341 ttagctttat cccagcccat gatttgcggc ggtacgacgc caatatagcc aatcttcagc
     8401 gtctgttttt ttccgtcttt atcgaccact tcggtgtctt taattaaata cggtgtaaac
     8461 attggctgtt tggttctggc gtcaatgacg ttggcattta cataagggaa tttcgctccc
     8521 gccagcgcat ttttcaggta atccagaccg tagttaaact catggttgcc aagcgtgccg
     8581 acggtatagt ccagcgtatt taatgcctta tacactgggt gaatatcacc tgcttttaat
     8641 cctttcgccg acatgtaatc tgccagcgga ctcccctgaa tcaaatcgcc gttatcgacc
     8701 agtacgctgt ttttcacttc attgcgggca tcgttaatca ggcttgccgt acgtaccagt
     8761 ccgaattttt ccgtggcggt gtctttgtaa taatcgaaat ccatcatgtt gctatgcaga
     8821 tcagtggttt ccatgatacg cagatcgacc gtcgctgcat tcacactggc ggcaatcagc
     8881 gtggccagga gcgttgcgct aaacttaatc atcagggaca tccttttatc atcgggaata
     8941 cgaaagaaaa gggagaataa acgtcttact tatagaacag tgaagaatgc cacaatttta
     9001 cgctttgaaa atgatgacac tatcacagtt ggcgcattca ttaacgatag ggtataagta
     9061 aaacaataag ttaacaccgc tcacagagac gaggtggaga aatgttagat caagtatgcc
     9121 agcttgcacg gaatgcaggc gatgccatta tgcaggtcta cgacgggacg aaaccgatgg
     9181 acgtcgtcag caaagcggac aattctccgg taacggcagc ggatattgcc gcacacaccg
     9241 ttatcatgga cggtttacgt acgctgacac cggatattcc ggtcctttct gaagaagatc
     9301 ctcccggttg ggaagtccgt cagcactggc agcgttactg gctggtagac ccgctggatg
     9361 gtactaaaga gtttattaaa cgcaatggcg aattcaccgt taacattgcg ctcattgacc
     9421 atggcaaacc gattttaggt gtggtgtatg cgccggtaat gaacgtaatg tacagcgcgg
     9481 cagaaggcaa ggcctggaaa gaagagtgcg gtgtgcgcaa gcagattcag gtccgcgatg
     9541 cgcgcccgcc gctggtggtt atcagccgtt cccatgcaga tgcggagctg aaagagtatc
     9601 tgcaacagct tggcgaacat cagaccacgt ccatcggctc ttcgctgaaa ttctgcctgg
     9661 tggcggaagg acaggcgcag ctgtacccgc gcttcggacc aacgaatatt tgggacaccg
     9721 ccgctggaca tgctgtggcc gcagctgccg gagcgcacgt tcacgactgg cagggtaaac
     9781 cgctggatta cactccgcgc gagtcgttcc tgaatcctgg gttcagagtg tctatttatt
     9841 aattaattta tgcagcaggt ccatcacttg ctgcacctct tcctgagtaa gcgccccgtc
     9901 tttggcccat tgcacgcgac cgtctttatc cagcaccact acagcggaac ttttttcatc
     9961 cagctgccag gcaccgcgtg cgacgccatt gctatcaaca ataaactgcg accagggata
    10021 aagcttttta ttactctcca gactgctgcg cacaaacatg ccggaacccg gaattgcgtc
    10081 gtcggtgtta acaatggtgg tggtctggta acgatcgtgc ggtaacttcg ctgatttaat
    10141 cgcttcaatc agcgtcgcgt ttttctcttt tgcagaggtg cgaccagcaa tatgttgcag
    10201 tactcgcact tttcccacta actgcgcgct gttccaggtt ttgtagctaa actgatcttt
    10261 atcaagcacc aactcgccac gatcggtgat gccaatcggc ggcactcgct gaccggtttc
    10321 gaactgatgt gcggaagcca tcatcggcaa cagcaggcag gtgagtgcca gaatcttgcg
    10381 tagggtcatg gtgtttcctt cttatgatat gcaggtgatc cgaccacttg ggtctgagtt
    10441 ttaatcatat gtgctattta tcgaatttcc cgcaagtgtg atgccagttt gcggtcaagc
    10501 gcacaaatca tatgaaaaat gaatgcttat actgaagacc gcgcttcggt aaaaagataa
    10561 ttctgaataa ttgtaacctt taggtaaaaa aagttatacg cggtggaaac attgcccgga
    10621 tagtctatag tcactaggca ttaaaatttg cgcctcataa tagttgggcc gattgtggca
    10681 ccgcacaggc gtaatactca gcaggagata acaatgaaaa ttttccaacg gtacaaccca
    10741 cttcaggtgg cgaagtacgt aaagatcctg ttccgtggac ggttatacat caaggacgtt
    10801 ggcgcttttg aatttgataa gggtaagatt cttatcccaa aagtgaagga taagctgcat
    10861 ttgtcagtga tgtccgaagt taaccgtcag gttatgcgtc tgcaaacaga gatggcttaa
    10921 ccaaagtgct atgcagtaat aagacggctc ctgattcagg agccgttgat gtttctgggg
    10981 gttacgcgac gctttcttct ttatctttag cgtcaggcag ttttggcgaa agggcggtgg
    11041 ccttgctgtc gatccgggtc accagcagct ggtcgatgcg gtagttatcg atatccacca
    11101 cttcaaattt gtagccggcg aatttcaccg aatcggtgcg tttcgggatc ttacgcagca
    11161 taaacatcat aaagcctccg atggtttcgt agttgcccga ctgcgggaac tcgtcaatat
    11221 ccagcacgcg catgacgtcg tcaattgggg tgccgccgtc aatcagccat gagttctcat
    11281 cacgggcgac aatctgctct tccagcccct gaccgaccag atcgcccatc agcgtggtca
    11341 tcacgtcgtt gagggtgatg atccccacca ccagcgcgta ctcgttcatg atcaccgcga
    11401 agtcttcacc tgcggtttta aaactttcca acgcctctga aagggttaac gtatccggca
    11461 caatcagcgt gttgcgaatt tgtacgccgc tgttcagtgc caggctttgg ttagccagca
    11521 cgcggttcag caggtcttta gaatcgacat aaccgatgat gtggtcaata tcttcattac
    11581 agacgaggaa cttagagtgc ggatgttccg ccaccttatt cttcaggctt tgctcatctt
    11641 cgtggagatc aaaccaaatc acgttttcac gcggtgtcat tgaagacgga acggtacggg
    11701 attccagctc aaagacgttt tcaatcagct cgtgttcctg tttacgtaac acgcccgcca
    11761 gcgcaccggc ttccactacc gcgtagatgt catcagaagt gatgtcatct ttacgtacca
    11821 ttggcagttt gaaaatacgg aagatcatgt tcgccaggcc gttgaagaac cacaccagcg
    11881 gggtgcaaac gtacaggcag aagcgcatcg ggttgatgat acgcaaagcc accgcttctg
    11941 gcgcaatcat accgatgcgt ttcggggtta aatccgcaaa caggataaac atgccagtca
    12001 ctaacgagaa agagagaata aagctcagtt gctcagagag ttctgccgac atatagcggg
    12061 agaacaggct gtgaaaagct ggagaaaatg ccgcatcacc gacgataccg ccgagaatcg
    12121 caactgcgtt cagaccgatt tggaccaccg taaagaacat gccgggattt tcctgcatat
    12181 tcagaacgcg ttgggcattt atattgcctt catcagccag cagtttaagt ttgattttgc
    12241 gtgaggcggc aagtgagatc tcagacatcg agaagaacgc acttacagcg atcaagcaga
    12301 gtatgactaa aatactgttt aacatatctt atccgacctt tcaggccaga tcctcggaag
    12361 gggaagttga ttaatttgtg tgaaatacac attgaaagcc gattcgaaga aagtgaaccg
    12421 gcattttcag tggctagtat agcgtaaggt actgtaaagt cgccagaggg ttaaaattca
    12481 gactgctgat aaatgcaata ctgcctgatg cgctatgccg atcaggccta cgcagtccct
    12541 gcaatatatt gaatttggac gattttgtag gcctggtaag gcattcacgc cgcatctggc
    12601 ataaataaag cgcactttat caacaatcta catttgtacc cgtaacgcta tgcttccggt
    12661 ggcagacaga cgccaattcc gccaattcca cagtaaccat acgggttttt atgcagatat
    12721 tgctggtggt catcttcggc ataataaaac ggtgtggcgt tagcgatttc cgtggtgatg
    12781 tgacgatcgt catcggcggc aagcatcgcc gcctgaaaac gttccagact ggcgcgagct
    12841 gcggcatcct gttctggggt cagcggataa atcgctgaac gatactgcgt gccgtggtca
    12901 ttgccctgac gcattccctg ggcgggatcg tgattctccc aaaatacctg tagcaactgc
    12961 tcatagctga tgacggaagg atcgtaaaca atgcgtaccg cttcggcatg acccgtatca
    13021 ccggagcaca cttcccgata agtcggattt ggcgtatagc ctccggtata gcctgcggcg
    13081 gtgctgtaaa cgccgggtaa ctgccagaac agacgctcca ctccccagaa acaacccatc
    13141 gcaaaaatgg caatctccat tccgtcaggt acattggtca ttgagtgacc gttgaccgca
    13201 tgcagcgtgg ctacgggcat cggggtgtta cgtccaggca gggcatcggc gggggaaacc
    13261 agatgctttt tatcaaataa actcatggtg tcgctctccc gaaaatcggt cattggggtt
    13321 aaggttgtaa caagaggcgt atttgcacac aataaccacc gtgaataggt ctaaagtaaa
    13381 acataagaaa tatttggggt ttagtctgct ttttaatcca tattactgga tttttgttaa
    13441 gccgtttaac ggcgttccag gggcaggaaa aaaggatatt caggagaaaa tgtgcgctat
    13501 atccgacagt tatgctgtgt aagcttactc tgcttaagcg gatctgccgt cgccgcgaac
    13561 gtccgtctac aggtcgaggg gttatcggga cagctggaaa agaacgttcg tgcgcagctt
    13621 tctacgattg aaagtgatga agtgacgcca gaccgtcgct ttcgcgcacg cgtcgatgat
    13681 gccatccgcg aaggtctgaa agcgctgggt tattaccagc cgaccattga atttgatctc
    13741 cgtccaccgc caaagaaagg gcggcaggta ttgatcgcca aagtcacgcc aggtgtgccg
    13801 gtgttaattg gcggcaccga tgtggtattg cgcggcggcg cgcggaccga taaagactat
    13861 ttgaaattgc tcgatactcg cccggctatt ggcacggtgc tgaaccaggg cgattatgaa
    13921 aatttcaaaa agtccttaac cagcattgcg ttgcgtaaag gttatttcga tagcgaattt
    13981 accaaagcgc agctgggcat tgcgctcggc ctgcataaag ccttctggga tattgattat
    14041 aacagtggcg aacgttaccg ctttgggcat gtgacctttg aaggatcaca aatccgcgat
    14101 gaatacctgc aaaatctggt gccgtttaaa gagggcgatg agtacgaatc gaaagatctg
    14161 gcggaactga accgccgact ttctgctacc ggctggttta actcggtggt ggtggctcca
    14221 caatttgata aagcgcgcga aacgaaagta ttacccttga cgggcgtggt ttcgccgcga
    14281 actgaaaaca ccatcgaaac cggggtcggt tactctacgg acgtgggacc gcgcgtgaaa
    14341 gcgacgtgga aaaagccgtg gatgaactct tatggtcaca gtctgaccac cagtaccagt
    14401 atttccgcgc cggaacagac cctcgacttc agctataaaa tgccgctgct gaagaatcca
    14461 ctggaacaat attatttggt gcagggcggt tttaagcgca ctgacctgaa cgataccgaa
    14521 tctgactcca ctacgctggt ggcttctcgc tactgggatc tctccagcgg ctggcagcgt
    14581 gccattaacc tgcgctggag cctcgaccac tttacccagg gtgaaattac caacaccacg
    14641 atgctgtttt atcctggggt gatgattagc cgcacgcgtt ctcgtggtgg cctgatgcca
    14701 acctggggcg actcgcaacg ctactctatc gactactcca acacggcctg gggttcagat
    14761 gtcgatttct ccgttttcca ggcgcagaac gtctggatcc gcacactgta cgatcgccat
    14821 cgtttcgtta cacgcggcac gctgggctgg attgaaaccg gtgatttcga caaagtaccg
    14881 ccggatctgc gtttcttcgc cgggggcgat cgcagtattc gcggctacaa atacaaatct
    14941 atcgccccga aatacgccaa cggtgacctg aaaggggcct cgaagttgat aaccggatcg
    15001 ctggaatacc agtacaacgt gaccggaaaa tggtggggcg cggtgtttgt cgatagtggc
    15061 gaagcggtaa gcgatattcg ccgcagcgac tttaaaaccg gtaccggggt cggcgtgcgc
    15121 tgggaatcgc cggtcgggcc aatcaaactc gattttgccg taccggtcgc ggataaagac
    15181 gaacacgggt tacagtttta catcggtctg gggccagaat tatgagttta tggaaaaaaa
    15241 tcagcctcgg cgtggttatc gttatcttac tgttgctggg atcggtggcg tttctggtgg
    15301 gcaccaccag cggcctgcat cttgtattta aagcggcgga tcgctgggtg ccaggactgg
    15361 atattggcaa ggtcaccggc ggctggcgcg atctcacctt gtctgacgtt cgttatgagc
    15421 agccaggcgt ggcggtaaaa gcgggtaatc tgcatctggc ggtcggtctt gagtgcctgt
    15481 ggaacagtag cgtttgtatt aatgacctgg cgctgaaaga cattcaggtc aacatcgaca
    15541 gtaaaaaaat gcctccttct gaacaggttg aagaagagga agatagcggt ccgctggatc
    15601 tctccacgcc gtatcccatc accctgacac gggtggcgct ggacaacgtc aacatcaaga
    15661 ttgatgacac cacggtgtcg gtgatggact tcacctccgg cctgaactgg caggagaaaa
    15721 ccctgaccct gaaaccgacg tcgctgaaag gcctgctgat tgctctgccg aaagtggcgg
    15781 aagtggcgca ggaagaagtg gtcgaaccga aaattgaaaa tccgcagccg gaggaaaagc
    15841 cgctcggcga aacgctgaaa gatctctttt ctcgcccggt attgccggaa atgaccgacg
    15901 tgcatttgcc gcttaacctg aacattgaag agtttaaagg cgagcagtta cgcgtgacgg
    15961 gcgacacgga catcaccgtg cgcaccatgc tgctgaaagt gagcagcatt gacggcaata
    16021 ctaaactgga cgccctggat atcgattcca gtcaagggat cgtcaacgcc agcggcacgg
    16081 cgcagctgtc agacaactgg ccggtggata tcaccctcaa cagcacactg aacgtggagc
    16141 cgttgaaagg agaaaaactg aagctgaaag tgggcggtgc gctgcgcgaa cagctggaga
    16201 ttggcgttaa cctttccggt ccggtggata tggatttacg cgcccagacg cgactggcgg
    16261 aagccggatt gccgctcaac gtggaagtga acagcaaaca gctttactgg ccgttcactg
    16321 gtgagaagca gtatcaggcg gatgatctga aactgaaact taccggcaaa atgaccgatt
    16381 acacgctctc tatgcgtacg gcagtgaagg gacaggagat cccgccagcc accattaccc
    16441 ttgatgccaa aggtaatgaa cagcaggtca atctcgacaa actcaccgtc gcggcgctgg
    16501 aagggaaaac tgaactcaag gcgttgctcg actggcagca ggccattagt tggcgcggtg
    16561 agctaacgct taatggcatt aacaccgcca aagagttccc ggagtggccg tcgaaactca
    16621 atggcttgat taaaacccgc ggtagcctgt acggcggcac ctggcagatg gaggtgccag
    16681 aactgaagct gaccggtaac gtcaaacaga acaaagtgaa cgttgacggc acgctgaaag
    16741 gcaacagtta tatgcagtgg atgatcccag ggctgcatct ggaactcggg ccaaacagtg
    16801 ccgaagtgaa aggcgagctg ggggtaaaag atctcaatct tgatgccacc atcaacgcgc
    16861 cggggctgga taacgcgctg ccggggcttg gcggtacagc gaaagggctg gtgaaagtac
    16921 gcggcacggt ggaagcgcca caactactgg cagatatcac cgcgcgcggc ctgcgctggc
    16981 aggaactttc cgtggcgcag gttcgcgtgg aaggcgacat taaatccacc gatcagatcg
    17041 ccgggaaact cgacgtacgc gttgagcaaa tttcgcagcc ggatgtaaat atcaacctcg
    17101 tcaccctgaa tgccaaaggc agcgaaaagc agcacgagct acagttgcgg attcagggcg
    17161 agcctgtctc cgggcagctt aatctggcag gaagttttga tcgcaaagaa gaacgctgga
    17221 agggaactct tagcaatacc cgcttccaga cgccggtcgg cccgtggtcg ctgacccgcg
    17281 atattgcgct ggattaccgc aataaggagc aaaaaatcag catcgggcca cactgctggc
    17341 ttaacccgaa tgcggaactg tgcgtgccgc aaactatcga tgcgggggcc gaagggcgtg
    17401 cggtggtgaa tctcaaccgc ttcgacctcg ccatgctgaa accgtttatg ccagaaacca
    17461 ctcaggccag cggtatcttc acgggtaaag cggatgttgc ctgggacacc acgaaagagg
    17521 ggctgccgca gggcagtatc accctttcgg ggcgtaacgt gcaggtaacg caaaccgtca
    17581 acgatgcggc gctgcccgtg gcgtttcaga cactgaatct gacggcggaa ttgcgtaaca
    17641 accgtgccga attgggctgg accatccgcc tgaccaataa cggccagttt gatggacagg
    17701 tgcaggtgac cgatccgcaa ggccgccgta atcttggtgg caacgtcaat atccgtaact
    17761 tcaaccttgc gatgataaac cccatcttta accgtgggga aaaagcagcg gggatggtga
    17821 gtgccaactt gcgtctgggt ggtgatgtgc aaagcccgca gttgtttggt cagcttcagg
    17881 ttacgggtgt ggatatcgac ggcaacttta tgccgtttga tatgcagccg agccagcttg
    17941 cggtcaactt taacggtatg cgctcgacgc ttgccggtac agtacggacc cagcagggtg
    18001 aaatctacct gaacggtgat gccgactgga gccaaattga aaactggcgg gcacgagtaa
    18061 cggcgaaagg cagtaaagtg cggatcaccg tgccgccgat ggtacgaatg gatgtatcgc
    18121 cagatgttgt attcgaggct acaccaaacc tgtttaccct cgatggtcgc gtggatgtcc
    18181 cgtgggcgcg catcgtggtg cacgatctgc cggaaagcgc agtaggcgtc tccagcgatg
    18241 tggtgatgct taacgataac ctgcaaccgg aagagccgaa aacggcgtcg attccgatta
    18301 acagtaacct gattgtccac gttggcaaca atgtgcgcat tgacgccttt ggcctgaaag
    18361 cgcggctgac gggcgatctt aacgtcgttc aggacaaaca agggctgggc ctgaacgggc
    18421 agatcaacat ccctgaaggg cgcttccatg cctatggtca ggatctgatt gtgcgtaagg
    18481 gtgagttact gttctctggt ccgccggatc aaccgtatct taatattgaa gctattcgta
    18541 acccggatgc tacagaagac gacgtaatcg ccggagttcg cgtcactggt ctggcggacg
    18601 aaccgaaagc ggagatcttc tctgacccgg cgatgtcgca acaagctgca ttgtcttatt
    18661 tgctacgtgg acaagggctg gagagcgatc agagcgacag tgcggcaatg acctcgatgc
    18721 tgattggtct gggggttgcg caaagtggcc agattgtggg taaaatcggc gagacgtttg
    18781 gcgtaagcaa tttagcgctc gacacccagg gagtaggcga ctcctcccag gtagtagtca
    18841 gcggctatgt attgccaggt ctgcaagtga aatacggcgt gggtatattt gactctatag
    18901 caacactcac gttacgttat cgcctgatgc ctaagctata tctggaagcc gtgtctggtg
    18961 tagaccaggc actggatttg ctctatcagt tcgagtttta gcaatgcgaa tatttgtcta
    19021 cggcagttta cgccacaaac aaggcaacag tcactggatg accaatgccc agttactggg
    19081 cgatttcagt atcgataact accagttgta tagcctgggc cactatccag gcgcagttcc
    19141 ggggaacgga acagtacacg gtgaagttta tcgtattgac aacgccacgc tggccgaact
    19201 tgatgccttg cgcaccaggg gcggtgaata cgcgcgccag ttgattcaga cgccgtacgg
    19261 gagtgcatgg atgtacgttt atcaacgacc cgtcgatgga ttaaagctaa ttgaaagcgg
    19321 cgactggtta gacagggata agtaaccata tgcatacgcc accttcgggt ggcgttgttt
    19381 tttgcgagac gactcgcatt ctgttttgta attccctcac cctttgcttt tctctccgag
    19441 ccgctttcca tatctgttaa cgcataaaaa actctgctgg cgttcacaaa tgcgcagggg
    19501 taaaacgttt cctgtagcac cgtgagttat actttgtata acttaaggag gtgcagatgc
    19561 gtattaccat aaaaagatgg gggaacagtg caggtatggt cattcccaat atcgtaatga
    19621 aagaacttaa cttacagccg gggcagagcg tggaggcgca agtgagcaac aatcaactga
    19681 ttctgacacc catctccagg cgctactcgc ttgatgaact gctggcacag tgtgacatga
    19741 acgccgcgga acttagcgag caggatgtct ggggtaaatc cacccctgcg ggtgacgaaa
    19801 tatggtaaag aaaagtgaat ttgaacgggg agacattgtg ctggttggct ttgatccagc
    19861 aagcggccat gaacagcaag gtgctggtcg acctgcgctt gtgctctccg ttcaagcctt
    19921 taatcaactg ggaatgacgc tggtggcccc cattacgcag ggcggaaatt ttgcccgtta
    19981 tgccggattt agcgttcctt tacattgcga agaaggcgat gtgcacggcg tggtgctggt
    20041 gaatcaggtg cggatgatgg atctacgcgc ccggctggca aagcgtattg gtctggctgc
    20101 ggatgaggtg gtggaagagg cgttattacg cttgcaggcg gtggtggaat aaggtgtgtt
    20161 tatttatcgc gggcataaaa aaacccttac taaccgaagc tcggcgttca gggttattgc
    20221 gctagaagaa cttatttatt ctttgcgcgc tcgaaggaag caacgatttc agctttagcg
    20281 gcttctgcgt tttcccaacc ttcaactttc acccacttgc ctttttcgag gtctttgtag
    20341 tgctcgaaga agtgagcgat ctgcgctttc agcagctctg gcagatcgtt aacgtcttta
    20401 atgtgatcgt attctttgct cagcttgctg tgcggaaccg caaccagttt cgcatcttca
    20461 ccggcttcgt cggtcatttt cagaacgcca accggacggc aacggatcac agaacccggc
    20521 tgcagcgggt acggagtcgg gaccagtacg tcaaccgggt caccgtccag agacagggtg
    20581 tggttgatgt aaccgtagtt gcacggatag aacatcgcgg tggacatgaa gcggtcaacg
    20641 aacagtgcgc cgctctcttt gtcgatttcg tatttgatcg gatctgcgtt agccgggatc
    20701 tcaataacca cgtagatgtc ttccggcaga tctttacccg cagggacgtt gagtaagctc
    20761 atgtctgttt cctttaaaaa tatgtggcaa acaagtgccg agtattatag ccaactcgcg
    20821 ccgaatgtct ttgcttgttt tcgtcttcat tttccttttt cgccagtttt aagacggtat
    20881 acagagcagg aaaatccata accgtagccg catttttcat agtgagatga aagcgattac
    20941 aaacttgtga ttaacgtttt atttactttt ttgaagtgtg atgtaacgca atccgttaca
    21001 taacgaattg tctatagttt tttcgcgaac atcttttcac caataataat taccccgacg
    21061 aggacaaccc tatgtggaaa cgcttacttg tagtctctgc agtctcggca gccatgtcgt
    21121 ctatggcgtt ggccgctcca ttaaccgtag gattttcgca ggtcggatcg gaatccggct
    21181 ggcgcgctgc agaaaccaat gtggcgaaaa gtgaggccga aaaacgcgga attacgttga
    21241 aaattgccga tggtcagcaa aagcaggaaa accagattaa agcggtacgt tccttcgttg
    21301 cacaaggggt ggatgcgatc tttatcgctc cagtggttgc gacgggttgg gaaccggtat
    21361 taaaagaggc gaaagatgcc gaaatcccgg tcttcttgct tgaccgttcc atcgatgtga
    21421 aagacaaatc tctctatatg accaccgtca ctgccgacaa catcctcgaa ggcaagttga
    21481 ttggtgactg gctggtaaaa gaagtgaatg gcaaaccatg caacgtggtg gaactgcagg
    21541 gcaccgttgg ggccagcgtc gccattgacc gtaagaaagg ctttgccgaa gccattaaga
    21601 atgcgccaaa tatcaaaatc atccgctcgc agtcaggtga cttcacccgc agtaaaggca
    21661 aagaagttat ggagagcttt atcaaagcgg aaaacaacgg caaaaacatc tgcatggttt
    21721 acgcccataa cgatgacatg gtgattggcg caattcaggc aattaaagaa gcgggcctga
    21781 aaccgggcaa agatatcctc acgggttcca ttgacggtgt acccgatatc tacaaagcga
    21841 tgattgatgg cgaagcgaac gccagtgttg aactgacgcc gaatatggca ggtcccgcct
    21901 tcgacgcgct ggagaaatac aaaaaagacg gcaccatgcc tgaaaagctg acgctgacca
    21961 aatccaccct ttatctgcct gataccgcaa aagaagagtt agagaagaag aaaaatatgg
    22021 ggtattgagg gttgctatgc ctgatgccga ttcgtaggcc ggataaggcg ctcgcgccgc
    22081 atccggcgat ggtgcactga agcctgatgc gacgcttacc gcgtcttatc atgcctactg
    22141 ggagcacgct ttacaccggg ggaaaccatg acgaccgacc aacaccagga gatcctccgc
    22201 accgaaggat taagtaaatt tttccccggc gtcaaagcgt tagacaacgt tgatttcagc
    22261 ctgcgccgtg gcgaaatcat ggcgctgctt ggtgaaaacg gggcgggaaa atcaacgcta
    22321 atcaaagcat taactggtgt ataccacgcc gatcgcggca ccatctggct ggaaggccag
    22381 gctatctcac cgaaaaatac cgcccatgca caacaactcg gtatcggcac cgtctatcag
    22441 gaagtcaacc tgctacccaa tatgtcggtt gctgataatc tatttatagg ccgcgaaccc
    22501 aaacgtttcg gccttctacg ccgtaaagag atggaaaagc gcgccaccga actgatggca
    22561 tcttacggtt tctccctcga cgtgcgcgaa ccgcttaacc gtttttcggt ggcgatgcag
    22621 caaatcgtcg ccatttgccg ggctatcgat ctctccgcca aagtgctgat cctcgatgaa
    22681 cccaccgcca gcctcgacac ccaggaagtg gagctattat ttggcctgat gcgtcagttg
    22741 cgcgatcgcg gcgtcagcct gatctttgtc actcactttc tcgatcaggt ctatcaggtc
    22801 agcgatcgga tcaccgtctt acgcaacggc agtttcgtag gctgtcggga aacgcgcgag
    22861 ctaccgcaga tcgaactggt aaaaatgatg ctggggcgcg agctggatac ccacgcgcta
    22921 cagcgtgccg ggcgaacatt gttgagcgac aaacccgttg ccgcgttcaa aaattacggc
    22981 aaaaaaggaa cgatagcacc gtttgatctc gaagtgcgcc ccggcgagat cgtcggtctg
    23041 gctggcttgc tgggatccgg acgtaccgaa accgccgaag tgatcttcgg tatcaaacct
    23101 gctgacagcg gcacggcgtt gatcaaaggc aaaccgcaaa ccctgcgatc gccacatcag
    23161 gcttcagtac tgggcatcgg attctgcccg gaagacagga aaaccgatgg catcatcgct
    23221 gccgcctcgg tgcgggaaaa tatcatcctc gctctccagg cccagcgcgg ctggctacga
    23281 cccatttccc gcaaagagca gcaagagatt gccgaacgct ttatccgcca gcttggcatt
    23341 cgcacacctt caactgaaca accgattgaa tttctctctg gcggcaatca gcaaaaagtg
    23401 ttgctttcac gttggctact gacccgaccg caatttctga tcctcgacga gccaacccgc
    23461 ggcattgatg ttggtgccca cgccgagatc atccgcctga ttgaaacgct gtgtgctgac
    23521 ggtctggcgc tgttggtgat ctcctccgaa ctggaagagc tggtgggcta tgccgatcgg
    23581 gtgattatca tgcgcgatcg caaacaggtg gcggagatcc cgctggcaga gctttccgtt
    23641 ccggcgatca tgaacgccat tgcggcgtaa ggagaacagt gtgatgcctc aatctctccc
    23701 ggacactacg ccgccgaaaa ggcgctttcg ctggccaacg ggaatgccgc agctggcggc
    23761 gctattgctg gtgctgctgg tcgatagcct ggtagccccg catttctggc aggtggtgct
    23821 tcaggatggg cgtttgttcg gtagccctat cgacattctt aaccgtgcag ctcccgttgc
    23881 gttactggcg attggtatga cgctggtgat cgccaccggt gggattgatc tctccgtagg
    23941 ggcggtgatg gctatcgccg gagccacaac ggctgcgatg acggtcgcgg gattcagcct
    24001 gccgattgtt ttgttaagcg ccctgggcac cggcatcctg gcgggattgt ggaacggcat
    24061 actggtagcg atcctcaaaa ttcagccgtt tgtcgccacc ctgatcctga tggtcgccgg
    24121 gcgcggcgtg gcgcaactga tcaccgccgg gcagatcgtc acgtttaact cgccggatct
    24181 ctcatggttt ggcagcggat cgctgttgtt cctgccaacg ccggtcatta tcgcggtgct
    24241 gacgcttctc ctgttctggc tgttgacccg caaaacggcg ctggggatgt ttatcgaagc
    24301 cgttggtatc aacattcggg cggcaaaaaa tgccggggta aacacgcgga tcatcgtcat
    24361 gctcacctac gtgttgagcg ggctgtgtgc ggcgattgcg ggcattatcg tggcggcgga
    24421 tattcgcggt gccgatgcca ataacgccgg gttatggctg gagctggacg ccattcttgc
    24481 ggtggtgatt ggcggcggat cgctgatggg cgggcgcttt aacctgctgc tctcagtcgt
    24541 gggggcgctg attattcagg ggatgaacac cggaatttta ctttctggct ttccgccgga
    24601 gatgaaccag gtcgtaaaag cggtggtggt gctttgtgtg ctgattgttc agtcgcaacg
    24661 ctttatcagt ctgattaaag gagtacgtag ccatgataaa acgtaacttg ccgctgatga
    24721 tcaccatcgg cgtctttgtg ctgggctatc tctactgcct gacccagttt cccggttttg
    24781 cttccacaag agtgatctgc aatatcctga ccgataacgc ctttcttggg ataatcgccg
    24841 ttggcatgac ctttgtgatc ctctctggcg gtatcgatct ctccgtcggt tcggtgatcg
    24901 cctttactgg cgtgtttctg gcaaaagtga ttggcgattt cggcctctcg ccgctgctgg
    24961 cgtttccgct agtgctggtg atgggctgtg cctttggcgc atttatgggg cttttgatcg
    25021 acgccctgaa gatcccggca tttatcatta ccctggcagg aatgttcttt ttgcgcggcg
    25081 tcagctatct cgtttcagaa gagtcgatcc cgataaacca tcccatttat gacacgctct
    25141 caagccttgc gtggaaaatc cctggcggcg gtcgcttaag tgcgatggga ctgctgatgc
    25201 tggcggtggt ggttatcggc atattcctcg cgcatcgtac ccgttttggt aatcaggtat
    25261 acgccattgg cggcaacgca acgtcggcaa acctgatggg gatttctacc cgcagcacca
    25321 ctattcgcat ttatatgctc tccaccggac tggcaacgct ggcggggatt gtcttctcga
    25381 tttataccca ggccggatat gcgctggcgg gcgtaggtgt ggaactggat gctatcgcct
    25441 cagtggtaat tggcggtacg cttttgagcg gtggcgttgg aacggtatta gggacgcttt
    25501 ttggcgtggc gattcagggg ctaattcaga cttatatcaa ctttgacggt acgctgagtt
    25561 cctggtggac gaaaatcgcc atcggcattt tgctgttcat ttttattgcc ttacagcgtg
    25621 gattaacggt gctgtgggag aatcgtcaga gttcgccagt gacaagagtc aatattgcgc
    25681 agcgataaaa acgcctctcc gtgtggagag gcgcaggaga ttacgcgtcc gggaattcac
    25741 ggataaagcg ttcgacatct tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct
    25801 ggtgcagagt ttccgggatg atatccagaa tacgctcttt gccatcgctc gctttaccgc
    25861 ccgcttgttc cgccaggaat gccatcgggt tgcactcata cagcaaacgc agtttgccgt
    25921 ccgggtggct ggcggtgctt gggtagagat aaataccgcc tttcagcagg ttacggtgga
    25981 aatccgcgac cagtgaaccg atataacgtg aggtatacgg gcggttggtg gatttatctt
    26041 cttcctggca gaatttaatg tacttcttca ccccgttcgg aaacttaatg tagtttcctt
    26101 cgttgatgga gtaggttttg cctttctccg ggaagcgcat ccgttcctgg cacaggcaga
    26161 aaacgcccag cgaaggatcg taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca
    26221 gcatggtaga ggagccgtat accacgtaac ctgccgcaac ctgtttgtta ccaggctgga
    26281 ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac gcggcggtag atggagaaaa
    26341 tggtaccgac agagacgtta acatcgatgt tggacgagcc atccaggggg tccatcagca
    26401 ccacgtattt tgcgtgttca cagccttcaa agacgacaat ctcatcttct tcttcagagg
    26461 caatgcccgc aacgatatcg cgtgctttca gtgcggcttt cagtttttca ttagcgaaca
    26521 agtcgagttt ctgctgaacc tcgccctgca cgttctcagc accgctggca cccaggatat
    26581 caaccagtcc tgctttgttg atatcgcgat ggataatctt ggcgcccagt tttattgccg
    26641 acagcaaagc agtgagctca ccggtagcat gagaaaactc gtgctgcttt tcgacaataa
    26701 attcacctaa cgttttcata aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa
    26761 caatcttaac aaataatctc aatgttgcgc tcaggtgaat cgcgccagca aattgcggat
    26821 tatcctgaaa tgcgtttctc acttgcccga catatgcgta aaatgagcgg cagattaaaa
    26881 aaggatagtg acgtatgcgc attcatattt taggaatttg tggcacattt atgggcggtc
    26941 tggcgatgct ggcgcgccag ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc
    27001 cgccgatgag caccttactt gagaagcaag gcattgagct gattcagggt tacgatgcca
    27061 gccagctcga tccgcagccg gatctggtga ttattggcaa cgccatgacc cgtggaaatc
    27121 cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc
    27181 acgattttgt gctgcgcgac cgctgggtgc tggccgttgc cggtacacac ggcaaaacta
    27241 ccaccgcggg aatggcgacc tggattctgg aacagtgcgg ttacaaaccg ggatttgtga
    27301 tcggcggtgt gccggggaac tttgaggttt cggcgcgtct gggcgaaagc gacttctttg
    27361 ttatcgaagc ggatgagtat gactgcgcct tcttcgacaa acgctctaaa tttgtccatt
    27421 actgcccgcg tacgctgatc ctcaacaacc ttgagttcga tcacgccgat atctttgacg
    27481 acctgaaagc gatccagaaa cagttccacc atctggtgcg tatcgttccg gggcagggcc
    27541 gtattatctg gccggaaaat gacatcaacc tgaaacagac catggcgatg ggctgctgga
    27601 gcgagcagga gctggtgggt gagcagggtc actggcaggc gaaaaagctg accactgacg
    27661 cttctgaatg ggaagtcttg ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg
    27721 tgggcgaaca taatatgcac aatggcctga tggcgattgc ggcggctcgc catgttggtg
    27781 tagcgcctgc agatgccgct aacgcgctgg gttcgtttat taacgctcgt cgccgtctgg
    27841 agttgcgtgg tgaagcgaat ggcgtgacgg tatatgacga ttttgcccat cacccgacgg
    27901 caattctggc aacgcttgcg gcgctgcgtg gcaaagttgg tggtacggcg cgcattattg
    27961 ctgtgctgga gccgcgctcg aataccatga aaatggggat ctgcaaagac gatctggcac
    28021 cttcattagg tcgtgccgat gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg
    28081 tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg gagtggcgat gtggatacgc
    28141 tggcagatat ggtggtgaaa accgctcagc ctggcgacca tattctggtc atgagcaacg
    28201 gcggttttgg tgggatccat cagaaactgc tggatggtct ggcgaagaag gcggaagctg
    28261 cgcagtaatt cggcctcagc ctgagatagc attgccggat aaggcgttta cgccgcatcc
    28321 ggcatttgag catagtgcct gatgcgacgc ttgatgcgtc ttatccggcc tacaggtgca
    28381 ccgcatccgg caaaccatac tcacatcaac aacgaaaatt acccttcatt ctccgccagt
    28441 tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg gcttattccc ttctttctct
    28501 ttcttcgcgt tacggatcag agtacgcagc tgctgacgat cggcatcggg ccacagattc
    28561 aatacttcag cgatggcgtc atcaccttga tcgatcagac gatcgcgcag gttttccagt
    28621 ttatggaaca gcaccacctg ctggttgtga cggtttttca gcttatccag cgcctgacga
    28681 ataggctcta cgtcgcgctg gcgcagcatc ttaccgatga gctgcaactg gcggcggcga
    28741 ccttccatct taatacgctg ggcgagctca atagccgcgc gcagatccgc atctaacggg
    28801 atcttatcca gcgcgttttt ccccagatca acaatttccg cgccaaggcg ttttagctcc
    28861 tcggcatcac gtttaatttc acttttactg acccagataa tttcatcgtc ttcgtcttcg
    28921 atgtcatcac cgggaacgtc gtcgagccag tcttcgggct gcttagtcat ctcaggctcc
    28981 ttaaaaaaag aggctaatgt taccagttaa gatgcgcact gaaaaacggt tctctgttag
    29041 acttcagaga aactctctac attatggcac ttgcaatgaa agtaatctct caagttgaag
    29101 cgcagcgcaa gattctggaa gaagcagttt ccactgcgct ggagttggcc tcaggcaaat
    29161 cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat tagcgtaagc acgcgttatg
    29221 gtgaagtgga gaatgttgaa ttcaatagcg atggcgcgct ggggatcact gtttatcacc
    29281 agaaccgcaa aggtagcgca tcatccaccg atttaagccc gcaggccatt gcccgcaccg
    29341 tacaggcggc gctggatatt gcccgttata cctcgccaga tccctgtgcc ggcgtggcag
    29401 acaaagagct gctggccttt gacgcaccag atctcgactt gttccaccct gcggaagttt
    29461 ccccggatga agccattgaa ctggcggccc gcgcagaaca ggcggcattg caggcggaca
    29521 aacgcattac caataccgaa ggcggcagtt ttaacagcca ctacggtgtc aaagtttttg
    29581 gcaacagcca cggcatgttg cagggttact gctctacgcg tcattcactc tccagctgtg
    29641 taattgccga agaaaatggc gatatggagc gtgattacgc ctacaccatt ggccgcgcga
    29701 tgagcgatct gcaaacgcca gagtgggtcg gggccgactg tgctcgccgt actttatcgc
    29761 gcctgtcacc gcgtaaactc tccaccatga aagcgccagt catttttgcc aatgaagtgg
    29821 caaccgggct ttttggccat ctggtggggg cgatagcggg tggctcggtt taccgtaaat
    29881 ctacctttct gctggattcg ctgggtaaac aaattctgcc ggactggctg accattgaag
    29941 agcatccgca tctgctgaaa gggctggcgt cgacgccatt cgacagcgaa ggtgtgcgca
    30001 ccgagcgtcg cgatattatt aaagatggca tcctgactca gtggctgctg accagctact
    30061 cggcgcggaa actggggctg aaaagcaccg gacatgcggg cggtattcac aactggcgga
    30121 ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga gatgggcacc gggctggtgg
    30181 tgacggagct gatgggccag ggcgtgagtg ccattaccgg tgattattcc cgtggtgcag
    30241 cgggcttctg ggtagagaat ggcgaaattc agtatcccgt tagtgaaatc accatcgcag
    30301 gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg taacgatatt gaaacacgca
    30361 gtaatataca gtgtggttct gtgctgttgc cggagatgaa aatcgccggg cagtaaaaaa
    30421 ctggcgcgac ctgccgcgct ataaactaaa aaattacaca aaatcattcg cactgcatcg
    30481 agacggaaag tgaatgaggg ccggggagcg tactcgcagt acgtgacccg ggcgaatgag
    30541 cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa taaaaaagga agtgagcaat
    30601 gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg gtattcagtt ctgcgtcgtt
    30661 tgccgctgat cttgaagaca atatggaaac cctcaacgac aatttaaaag tgatcgaaaa
    30721 agcggataac gcggcgcaag tcaaagacgc gttaacgaag atgcgcgccg cagcgctgga
    30781 tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca ccggacagcc cggaaatgaa
    30841 agatttccgc cacggtttcg acattctggt cggtcagatt gacgacgcgc tgaagctggc
    30901 aaatgaaggt aaagtaaaag aagcgcaggc tgctgcagag caactgaaaa cgacccgcaa
    30961 cgcctatcac cagaagtatc gttaattcct catttccctg ttgcctgcac tcaggtaaca
    31021 gggaacttat catcgcaaat gatgcaccac ctgattgctg ctgccgcgcc agatcaggga
    31081 agggtctttt aaatcctgca caaatttgcc gtcgaccagt acgttaatca aatcaacaac
    31141 ctgcatttgc gcagcgttga gttcgtcgag tttatagcct gtccacaccc agatgtcttt
    31201 acccggacac tcggcgcgga tgcgttttac cagtttcaga atatccggca cgttttgcgg
    31261 atgcagcgga tcgccgccgg agagggaaat accctggcgt ttgatacgag tgtcattcag
    31321 atcgttaata atctggtctt ccattgcttt agtaaatgtc tgaccggaat ttacccgcca
    31381 tgtgcttttg ttatagcaac cggggcattc atgaacacac ccggagacaa acagggtgca
    31441 gcgagtgccg gggccgttga cgatgtcgac aggatagtac tgatgataat tcattgtttc
    31501 gcctggctta tcagtccgcg tataggatgc atccggtaat caacgcctga tgcgacgctg
    31561 tcgcgtctta tcagacctac aatcgttgtt gccatgtagg ccggataagg cgtttacgcc
    31621 gcatccggca tttgcttaac gtagagtgaa gattaaccta tctgcccatt ccccaaatgt
    31681 ttaacgcggc gcttaacttc ttcctgctta ccggcgttaa atggacgtgc atccgggcta
    31741 cctaaatatc cgcacacgcg gcgagtcacc gacacacggg aggcgtcatg gttaccacat
    31801 ttcgggcaag tgaagccttt gctggtgcac tcgaactcac cggtaaagcc acactcgtag
    31861 cactcatcaa tcggtgtatt ggtgccgtaa tacggtacat gctgatagct gtaatcccag
    31921 acatcttcca gcgccttcag gttgtgctga atgtttggat actcgccgta gcaaatgaaa
    31981 ccaccgttcg ctagcggcgg gtaaggcgct tcaaagtcga tcttgtcgta cgggttcacc
    32041 ttcttctcca catcgaggtg gaaactgttg gtgtagtaac ctttatcggt cacgcccggc
    32101 accacgccaa actcagcagt atcgagacgg cagaagcggt cgcacaggtt ttcactcggc
    32161 gtgctgtaga gactgaaacc ataacccgtt tcttctttcc actgatccac tgcctggcgc
    32221 agacgttcaa caatggcgat acctttcgcg cgaagctgct cgttgtcgta aacatgctcg
    32281 ccgccgaaca gcgcgttaat ggtttcgtgg atgccgatgt aacccagcga aatagacgca
    32341 cgaccgtttt tgaagatttc agaaacatca tcgtcagcat tcagacgtac gccacaagca
    32401 ccttccatat agaggatcgg ggcgacgcgc gctttcacgc cttcgagacg agcgatacgg
    32461 gtcatcagtg ccttacgtgc cagcaccaga cgttcatcca gcagcttcca gaaggtggct
    32521 tcatcgcctt ttgcttccag agcaatacgc ggcaggttca ggctgatcac gccgaggttg
    32581 ttacgaccat cgtggatctg ctcgccgttt tcattttccc acacgccgag gaagctgcgg
    32641 cagcccatcg gggttttaaa cgaaccggtg actttcacta cctgatcgta gttcaggata
    32701 tccggataca tgcgcttgct tgcgcactcc agcgccagct gtttgatgtc gtagttcgga
    32761 tcgccttttt tatggttcag gccatcgcga atcgcaaaca ccagtttcgg gaacaccgca
    32821 gttttacggt ttttaccaag gcctgcgata cggttacgca ggatagattc ctgaatcagg
    32881 cgcgactccc agctggtgcc cagaccaaaa ccaaaggtta caaacggcgt ctgaccgttg
    32941 gcggtgtgca gggtgtttac ttcgtactcc agcgactgga aggcatcgta gcactcttta
    33001 atggtacgag agttagcgta gccttcggcg tccgggatgc tccactcttc tgcggtcttg
    33061 cgatgtttgt tgtagctggc ggtgacaaac ggtgccagca cttcatcgat acggttaatg
    33121 gtggtgccgc cataaatatg gctggcaacc tgagcaataa tctgcgcagt taccgcggtt
    33181 gccgttgaga tcgacttcgg cggttcaatc tcggcgttcc ccattttaaa gccctgggtc
    33241 agcatgcctt tcaggtcgat caacatgcag ttgaacatcg ggaaaaacgg tgagtaatcg
    33301 agatcgtgat agtgaatatc gccacgctca tgtgcctgca ccacgtcacg cggcagcagg
    33361 tgctgacgtg catagtgttt agccacgatc ccggccagca ggtcgcgctg ggttggaatc
    33421 accttgctgt ctttgttggc gttttcgttg agtaacgagg cgttggtctg ctcgaccaga
    33481 ccacggatct cctggttcag gcgaccgcgt ttttcacgtt caatgtcgcg atcgtgacgg
    33541 tactcgatgt aagcgcgagc cagttgtttg tatggacccg acatcagctg gttttcaact
    33601 gcggtctgga tctcgttgat atccacctgg ttgcggccct gcatctgctc gctgacaacc
    33661 gcggcaacag tggcgcaata atcggcatca tcgacttccg ctgctttagc tgcacgcaga
    33721 atcgcttctt tgatgcgctc tgatttaaac ggcactttgc agccgtctcg tttcatcaca
    33781 tgcggtgtca tgatcgctcc atatttttaa gaacaggtta tccacagaaa ttgggaaagg
    33841 cgttttccgg ttttttcgtt tcgttttccg gtgctttccg caatcgccat ccgcttatcc
    33901 acaacgccgc acctttttcg tggggagctg tagtagcaat tatagtcgat taatacaaca
    33961 tattgggttg ggacgcattt taaagtctat atatagtgct ttgcatcaag gatgttttaa
    34021 ctttttttga tgtagctcaa agtaaaaagc agagcgtacg gatgacgggc gctacagcgt
    34081 tatgtaaatt ttttaatgaa tttgctggtt gaaaaatcaa caaaaacaac atgctgacag
    34141 acaaaacccc ggaatgaccg gcattaccgg ggcttaggga agatttactt ctgtaaccac
    34201 cagacagcct caaaaggccg taaattcatg gcacagggtt gtggtgaggc ttcttcgtag
    34261 ttatgcatca caagctgcca gttgccgcgc atttgccctg gctgccaggg ttggatctca
    34321 cggctaaggt tggcaatgac cagcaaggtt tgccccttcc attcacggcg atagcaccac
    34381 aatacagggc tgtttggcag cagatcctgg taattgcccc atgtcaggat ggcttcttgc
    34441 ttacgcagtg cgattaactt ttggtaggtg taaaacaccg aggactcatc ggccagcgcg
    34501 gcttctacgt tgatttgttg atagttatcg cccaggccaa tccacggttc gccagccgta
    34561 aacccggcat tatcgccgtt gctccattgc atgggcgtgc ggctgttgtc acgggattta
    34621 ctggcgagga ttgccaataa ctcgtcggca tcacgaccat cgttgcgcag ctcggcaaac
    34681 atattgaggc tctccacgtc gcgatagtca gtaatgcgcg tgaaatgcgg gttggtcatg
    34741 ccaatctctt cgccctggta gatatacggc gttccctgca tgccatgcag caccatcgcc
    34801 agcatttttg ccgcaggcac gcggtattca ccttcatcac caaagcgaga aacaatgcgc
    34861 ggctgatcgt ggttacacca gaacaaggca ttccatgcca cgttgtgcat tccttgttgc
    34921 cagtggcgga acaatgtttt caacgccaca aagtcaggtt tagccagcgt ccatttttca
    34981 ccaccgggat aatcgacctt caggtgatga aaattaaagg tcatcgacaa ttcactgcct
    35041 gtcagtgccg cgtatcgctg gcaatgctca aggctggtgg aggacatttc acctacggtc
    35101 attaacccgc gtggcgtaaa cacatcgcgg ttcatctcgt gcaaaaactc gtgtgctcgt
    35161 ggcccgtcgg tgtagaagcg acgcccgtcg ccatccaggt cttcagggaa acgcgggtct
    35221 ttggagatca gattcaccac atccaggcgc aacccgtcga ccccacgatc ggcccagaat
    35281 tcacagactt ttttcagctc tgcgcgtacc gctggattct cccagttgag atccgcctgt
    35341 tctggtgcaa agagatgcaa atagtactgt tcgctttccg catgccagcg ccacgcacta
    35401 ccgccaaatt ttgaacgcca gttgttcggt ggcgtttctg gttcgccatc gcgccagata
    35461 taaaactggc ggtaagggct ttctttgttc agcgcctcgc gaaaccaggc atgttgggta
    35521 gaggtatggt taaacaccat atcgagaatg atacgaatcc cgcgcgattt tgcctgcgtc
    35581 accagttcgt caaaatcgtc cagcgtgccg taggtgggat caatcgccgt atagttcgct
    35641 acatcgtaac cgttatcgac ctggggagag acataaaagg gggttagcca gatggcatca
    35701 acgcccagtt tatgcagata gtccaggcgt tggataacgc cacgtaaatc gccggtgccg
    35761 ctacccgtgg tgtcctgaaa actctttgga taaatctggt agataacgcc gttttgccac
    35821 cagtggggaa gattagtcat tacgttattc ctgcaaatgc gagggggcgc aattgcgccc
    35881 cgaagaaaat taaacaatgt ccagcgtgcc caggcggtat ttccgctgat agataaacga
    35941 ggtgagtaca atcgggatga tgatggcgat agccattgcc agcgcaaata cctgccagta
    36001 gctcggttga atcgagagaa ttcccggcag gccgcctacg ccgatgccat tcgccataac
    36061 gccgttcagg ccgcatagca atcctgccag accagaacca atcatcgcgc acagcatcgg
    36121 gaagcgatat ttcaggttga tgccgtacat tgcaggctca gtgaccccaa gccaggcgga
    36181 gatagcggca ggcacggaga tctcgcgttc attgtgcttg cggctggaaa tgataatgcc
    36241 tatcacggcg gagccctgag cgatattcga cagcgcaatc agcggccaca ctggcgtgcc
    36301 acccatgctt tgaatcatct gcaaatcaat agcaagggtg gtctggtgta cgccggtgat
    36361 caccagcggc gcgtacagga agccaaacaa tgccgcacca atcggagcaa agttgccggt
    36421 catcaggtga cgtaccgcaa aggcaacgcc atcgccaatc atgcgaccaa acggaccaat
    36481 cagcgcatgg gcgaggaaca ccgcgaggat cagcgaacag acgggtacca ccaccagata
    36541 gaggtaatcc ggcacgatgc gtttaaggcg agtttcaata acgcccagcg ccagcccggc
    36601 taacagtgcc gggatcacct gcgcctgata gcctactttg gcgatgctga acatgccaaa
    36661 gtcccacact tccggcagct gctgcccgag cagataagcg ttcatcagct ggggagaaac
    36721 cagtgtcacg ccaagcacga taccaaggat cggcgtgccg cccatttttt tcaccgctga
    36781 ccagcaaata ccgaccggca ggtagaagaa gatcgcttca ccgatcaacc acagaaaatc
    36841 gtagatcgtt tgcagggaag ggtacatttg cgccagcgtc tgaccgttgc tcatgggcaa
    36901 atcgccgatc acattgcgaa aaccgaggat caaaccgccg ctaatcaacg cgggcagcag
    36961 cgggaagaag atctccgcga aatgagagat caactgctca tgccatttca tattctgccg
    37021 ggcggctttt tttacctgct ctttatcaac ctgcgcctgt ccggttgacg caatcagtgc
    37081 ttgatagtaa tcacccacat tggtgccaat caccacctga aattgcccgg cattggtgaa
    37141 acagcctttc accatgggga gttgctcaat ttctttcggt ctggcattgg ccggttggtt
    37201 gaggacaaag cgcaggcgag taatacagtg gctcaccgtc gcaatattgc cgcgcccgcc
    37261 gaccagttca atcaaccgat cgatatccgt ttggtttatt ttgctcatca taaagcccca
    37321 tggcagatga catttttggt tggctgcaga atatattgcg cggaaaaatt taaaaacggg
    37381 aacgttcccg aaacgcagcg aagatcacaa tttatcgttc aggaaacgat caggacaggg
    37441 tggcggggat gatgatttgt tgcggttcgc tgcgcccggt tacctgtgcg atcaattggc
    37501 aggccgcctg gcgtccagct tcggcgtaac cgggatctac ggtgacgatc tccggatgga
    37561 ggaatttcat taacggtgta ttaccgacgc tcgccagttg caaggtgtcg atgcgttgct
    37621 cttgcaggta tttacttgcg ccaagtgcca gcgtgtcggt tgcgcacagt aacgcagtag
    37681 tttcaggcgt aatcactttt gccacgttct catagccttg cttcatagca agccctggca
    37741 gagcggcaac gggatgcagt ttatgcgctt tgcagaacgc caggtaggct tcgtgacgtc
    37801 gcttaccggt tgtcacgtca ctatgcggca cgccgagata actgatatta cgatgcccct
    37861 ggtcatacag ccgttgcatc aggatcttga ttgccccttc gtcgtcataa cagaccgaag
    37921 caaagccttt tgcgtcacgc gccagcagaa ccagcgatga ctgccagtgg gctaacattt
    37981 cttctgttat gccagtaaaa ccgaacagca ctacgccgtc gatattacgc cgtttcagca
    38041 cccccaaatg ttcggcaact aattgtgggg aaaactgact ttccatcatg attgggtcgt
    38101 aaccttgttc atagaacgct ggcagcatgg tttgaacggc gagattttct gacaacgaat
    38161 ccagacgggt aacaatgatg gcgaccactt tatcgctttg cccacgcata gcgcgcgcag
    38221 agcgggaagg ggaaaatcca tgctgattca tcactgcttc aacacgctcg cgggtgcgct
    38281 ggctcacgcc gctttcgtta ttcagcaccc gggaaactgt agatttcccc acgccgctta
    38341 agcgtgcgat gtctttgatg gtcagccgat tttgcatcct gttgtcctgt aacgtgttgt
    38401 ttaattattt gagcctaact ttacccgtgc attcagcaat gggtaaagtc tggtttatcg
    38461 ttggtttagt tgccagcagg tattatatcg ccatagatgc tacgaatatt attggattct
    38521 ccttattatt tgcggcgctt ttttcactta ccggaggtta tatggaacct gatcccacgc
    38581 ctctccctcg acggagatta aaacttttcc ggtaagcccg tcttttcacg gcgttaccgg
    38641 atgcgtaagg ccgtgacgtt ttaacgtccc tgctcagctt tattaccttc aggtaaggct
    38701 tcgccacgcc tgaagacatt tctgtactgt ttcagacagt gcggagggac tccttatgtt
    38761 taaagaaatt tttacccggc tcattcgcca tttaccttcc cgtctggttc atcgcgatcc
    38821 attgcctggc gcgcagcaga cagtgaatac ggcggtcccg ccgtccttaa gtgcgcattg
    38881 cctgaaaatg gcggtgatgc ccgaagaaga attatggaaa acgttcgaca cccatccgga
    38941 agggttaaat caggcggaag tggaatctgc ccgcgaacaa catggtgaaa ataaattacc
    39001 cgcacaacaa ccgtcgccgt ggtgggtaca tttatgggtc tgctatcgca acccatttaa
    39061 tattttactc accattctcg gcgctatttc ttacgccacg gaagatttat ttgccgcggg
    39121 cgttatcgcg ctaatggtcg ctatttctac gttgctgaac tttattcagg aagcacgttc
    39181 cactaaagcg gcagatgccc tgaaagcgat ggtcagcaat actgcgacgg tgctgcgcgt
    39241 aattaacgac aaaggcgaaa atggctggct ggagatcccg atcgaccagc tggtgcccgg
    39301 cgatattata aaactggcgg cgggggatat gatcccggca gatttacgta tcttgcaggc
    39361 gcgggatctg ttcgtcgctc aggcgtcgtt aaccggtgag tctctgcccg tagaaaaagc
    39421 agctaccact cgccagccgg agcacagcaa tccgctggag tgcgacaccc tgtgttttat
    39481 gggcaccacc gtggtgagcg gcacggcaca agcaatggtg attgctacag gtgccaatac
    39541 ctggtttggt caactggcgg ggcgtgttag tgagcaggaa agcgagccga atgcctttca
    39601 gcaagggatc agccgcgtca gtatgctgct gattcgcttt atgctggtga tggctccggt
    39661 ggtgctgtta atcaatggtt acaccaaagg cgactggtgg gaagcggcac tgtttgcgct
    39721 ttcggtcgcg gtaggcttaa cgccggaaat gttgccgatg attgttacct cgacgctggc
    39781 gcgcggcgca gtaaagctgt cgaaacagaa agtgatcgtc aaacatctgg atgctattca
    39841 gaactttggc gcaatggata ttctgtgcac tgataaaact ggcaccctga cacaggacaa
    39901 aattgtgctg gagaatcata ccgatatctc cggtaaaacc agcgaacgcg tgctgcatag
    39961 cgcgtggttg aacagtcatt atcagaccgg acttaaaaac ctgctcgata cagcggtgct
    40021 cgaaggtacg gatgaagagt cagcgcgctc gctggccagt cgttggcaaa aaatcgatga
    40081 gattccgttt gatttcgagc gtcgccggat gtcggtggta gtggcagaaa ataccgagca
    40141 ccatcagctg gtttgcaaag gtgcattgca ggaaatcctc aatgtgtgtt cgcaggtgcg
    40201 tcacaatggc gagattgtgc cgctcgatga catcatgctg cgtaagatta agcgggttac
    40261 tgatacgctg aatcgtcagg ggctgcgcgt ggttgcggtg gcgacgaaat acctgccagc
    40321 gcgtgaagga gattaccagc gggcggatga atccgacctg atcctcgaag gatatattgc
    40381 tttccttgat ccgccgaaag agacaactgc tccggcactg aaggcattaa aagcgagtgg
    40441 aattaccgta aaaatcctta ctggcgacag tgagttagtt gcagcgaaag tgtgccatga
    40501 agtggggctg gacgcgggag aggtggtcat tggtagtgat attgaaacgc tatctgacga
    40561 cgaactggca aatctcgcac agcgtaccac gctgtttgct cgtctgacgc cgatgcataa
    40621 agaacgcatt gtgaccttac tgaagcgcga agggcatgtg gttggcttta tgggcgatgg
    40681 tattaatgat gcgcccgcct tacgcgctgc ggatatcggc atttctgtgg acggcgcggt
    40741 agatattgcc cgtgaagcgg ctgatatcat cctgctggaa aaaagcctga tggtgctgga
    40801 agagggggtt attgaaggac gtcgcacttt cgccaacatg ctgaaataca tcaaaatgac
    40861 ggcgagctcc aacttcggta atgtgttcag cgtgctggtg gcgagtgctt tcttgccctt
    40921 cctgccgatg ttgccgttac acttgcttat tcagaacctg ctgtatgatg tgtcacaggt
    40981 ggcgatcccg tttgataacg tcgacgacga gcaaattcaa aagccgcagc gttggaaccc
    41041 ggcggatctg gggcgcttta tgatcttctt cggaccgatc agctcgatct tcgatattct
    41101 gacgttttgc ctgatgtggt gggtattcca tgccaacacg ccggaaacgc aaacgctgtt
    41161 ccagtcggga tggtttgtgg tgggcttact gtcgcaaacg ctgattgtgc atatgatccg
    41221 cacccgccgt gtgccgttta ttcagagctg tgcatcgtgg ccgttaatga tcatgaccgt
    41281 gatcgtgatg attgtcggga tcgcattgcc gttttcaccg ctggccagtt atctgcaatt
    41341 acaggcgctg ccgttaagct atttcccgtg gctggttgcg attctggcag ggtatatgac
    41401 attaacccag ttggtgaaag ggttctatag ccgtcgttac ggctggcaat aaagaataaa
    41461 ctgggcacga tagccccgat tcagattgca gacaaagtaa gctttgttca tgccggatgc
    41521 ggcgtaaatg ccttatccgg ccaacaaatt caataaattg caggaatcac gtaggcctga
    41581 taagcgtagc gcatcaggcg attttgcgtt tgtcatcaga cttaatccgg gcatgatagc
    41641 ccggatttcc atcaagatta gcgacgaaca gcgatcgctt cgatctcaat cttcacgtct
    41701 ttcggcagac gggcgacttc aacgcaagaa cgtgccggga aggtggcgtt gtgttcggtg
    41761 aagaaggctt cgtaggtagc attaacggta gcgaagtcgt tcagatcttt cacaaacacg
    41821 gtggttttaa cgatgtcgcc cactttcagg ccagcggctt cgacgatcgc ttttacgtta
    41881 tccagcgact gacgtgcctg tgcagcgacg tctgccggta cttcacccgt tttcggattt
    41941 accgggatct gaccggaggt gatgatcata ttgcccagat caacgccctg tacgtaagga
    42001 ccgatagctg ccggtgcatt ttccgtcgcg atagttttgc tcatgatttc tcctttatta
    42061 cagcggtaaa aagtctggct cattataggg agccagactt ttattaccaa ccgcaattaa
    42121 ttggccagca ccacattatg ggaaaactct ttttcacagt atttgcattt gagcgcgatg
    42181 tcattggcgc gttttcgcac ggcaaagctg gaagaaaccg gttcggcatg gctgatacag
    42241 ttgctgttcg ggcagaccag cacattgtcg atgcgctctg gcagactggg gcgcgattta
    42301 cccaccactt catagttgtc gatacggtta accgtggctt gcggcgcata caatgccagt
    42361 tgatctacct gatcttcact caaaaaggta ttttcgattt tgatcagatc tttgcggccc
    42421 atctcgccag aaggcaagtt cagaccgatg gtgatgcgct gatccgtttc ggtcagcttg
    42481 aacagactca acagcttaaa accgatctgg gcggggatat ggtcaattac cgtgccgcgt
    42541 ttaatagctt caacctgcaa tttattatcg tgtgtcatct ctatttcccc ttacagtacc
    42601 agatcgcgat tcagaaccag tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc
    42661 tgctggaagt accaggcgtg tggcgtttta tccacatcgg tggcaatctc atcaacacgc
    42721 ggcagcggat gcagcacttt catattggct ttggcgttgt ggagatcgct ggcgcgaaga
    42781 acaaactgcg ctttcacgtt ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg
    42841 gtcatgtaca ggatgtctac ttccgccatc acttcttcaa tagagctgtg cagactccat
    42901 gcgatccctt tttcatcgag catatccaga atgtattgcg gcattgccag cgcgtccggc
    42961 gcgatgaagt aaaaacggtt gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg
    43021 gtgcggccat atttcaggtc accaaccatt gcgacgtgga gattgtccag acgcccctgg
    43081 gtttcctgaa tagtgaataa gtccagcaag gtttgcgtcg gatgttggtt ggagccatca
    43141 ccggcattca gtaccggtac attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc
    43201 tgcggatgac gcatcactat cgcatcgacg taagtgctga taaccgaaat ggtatcggcc
    43261 agcgtttcgc cctttttacc cagtgatgta ttggcgctgt cggagaagcc caccacgctg
    43321 gcacccaggc ggtgcataga tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag
    43381 aaacagctgg caatgacttt gtgcttcaac agctctggtt gcgggtttgc tttcagtttc
    43441 gccgctgtcg ccagcaccag attaaggtca tcgcgactaa ggtcgtttat ggaaatgata
    43501 tgtttctgat atagcggatt agccatcttt tatctcctga cgcctgggca aaaaaaagcc
    43561 cctcgattga ggggctggga atgggtgatc aacgggaaga aaaacggcag gccagcgtct
    43621 tttttcagac gcggtaagac aaaatgtcga acacactgaa ccatacatcc tcccggcaaa
    43681 ttgtccggca ttatactcat agccagaagc ggtgcaagca tttgatgcaa tattttgtca
    43741 gcgcaaacgg tttatttgaa ttaaaagtca aaatgtatgc atttttattt gctttgattc
    43801 tggaagagaa ctgtcgttcg gtatcaataa ttgcagacat ttcctgcttt tttctttttc
    43861 actttcacgc aatcagatat gcattttatt ccattcatcc gacttatacg gcggagtgtg
    43921 aaaacgaacg gctaacacta ttgcttacag ctcagggatg cgcgctatca ctttaatttc
    43981 aaaatcaaag cctgccagcc aggtaacgcc caccgccgtc cagtttggat aaggaggggc
    44041 gctaaatatt tcatttttga ccgtcatgat gtcttcaaat tgtttttccg gatcggtatg
    44101 aaagctcgta acatcaatga tatcgtcaaa agtgcatccc gcagctgcca gggtcgcatg
    44161 caaattatca aatgccagtc tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc
    44221 tcgacttcct acttgcccgg aaacaaacag caaatcgccg gaacgaatag ccgccgaata
    44281 acgatgttca gcatacagtg aatgtcggcc agcagggaaa acagcggttc tttctaccat
    44341 ttggttatcc tcaagattta cgacatgaac agaagattta tatttaccgg gagccgcttt
    44401 tagggaacga cgtgagtaaa caaaacccag acatcatgga taatggctgg gcttaattga
    44461 gcgtagtcgg ttatgcccca aacgcgccat caatggtatg catcgcgccg gtaacaaaac
    44521 tggcttctgg ccctgctaac catgcgacca ttccagcgac ctcttccggt tgcccatgtc
    44581 ttttgatagc cataaaacta tgcaacatat cgcgcattgg cccgttggcg ggattagcgt
    44641 cggtatctat tggccctggc tggacgacgt tgatggtgat cccacgcggt ccaaaatcac
    44701 gggccagccc gcgcgccatg ccttgcaggg cagatttact ggcggcataa gcagccatgc
    44761 ctgcaacagg catacgatcg ccattcacgg agccgatgat taagatgcgc ccgccttcgg
    44821 gcatctgccg ggcggcttca acagaggcat ggtaaggggc atgaatattg attttgaaaa
    44881 ggcgatcaat atcgtcggca tttaattcca gggcatcgcc aaagacgcca atacctgcat
    44941 ttaccaccag gatatccaat gcgccgctct tacgaacgac atcaataaca gcgtctctgt
    45001 cagcactatc tgtgaatact gctgtcgctc cagtctcatg tgccaggcgt tcagcggcat
    45061 ctttcgaccc cgcataggtg aatcgtacat tggctccatc ggtgacgaaa cgacgtacga
    45121 tagcggcacc tattccacgg ctaccaccga ggatgagaac tgtcttacct gtaaaagcgc
    45181 ccataaggac tccttgattt attatgtaac atgcattaca aaactgtttt aactttctgt
    45241 caacaagttt tgtaatggtc actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt
    45301 tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg
    45361 ctgtcagtgt tgctgaagtt actgattatc ttggtattaa ccccccgagt ctctacgcgg
    45421 cttttggcag taaggctggg ttatttagcc gtgtactcaa tgaatatgtc ggtacggaag
    45481 ctattccgct tgccgatatt cttcgtgatg atcgtccggt aggggagtgc ctggctgagg
    45541 tattaaaaga agcggcgcgc agatatagcc aaaacggcgg ctgcgctggc tgtatggttc
    45601 ttgaaggcat tcatagtcat gatccacaag cgcgtgatat tgccgttcaa tattatcacg
    45661 ccgcagaaac gaccatttat gactatatcg ccaggcggca tccacaaagc gcacaatgtg
    45721 tgactgattt tatgagtacc gtgatgtcag ggctttctgc gaaggcacga gaggggcact
    45781 ccctcgaaca actctgtgca acagctgcac tggcggggga agcgataaaa actattctca
    45841 aggagtgatg ctgaccttga tccgaaaggc gggaacgcgc ctgccgataa gttgtgataa
    45901 gacaataatt cacgcgttaa ggctggcgga attgatcatc ttttcgtata acggtggaaa
    45961 tgaaacattg ttttaattaa ggagtggaaa agatgatcat cggaaatatt cataatcttc
    46021 agccatggct accccaggag ttacgccagg cgattgagca tatcaaagca cacgttacgg
    46081 cagaaacgcc aaagggtaag cacgatatcg aaggcaatcg tctgttttat cttatctcgg
    46141 aagatatgac cgagccgtac aaagctcgcc gtgcggagta ccatgcccgt tatctcgaca
    46201 ttcagattgt gttaaaaggt caggaaggca tgaccttcag cacacaacct gcaggtacgc
    46261 cggagaccga ctggttagta gataaagaca tcgcattttt gccggaagtc gttgatgaga
    46321 aaacagttat cctgaatgaa ggtgattttg ttgtgtttta tccgggggaa gtgcataaac
    46381 cgctgtgcgc agtgggtgca ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg
    46441 cgtgatgact tttcgccgta aataactcca ggtttacggc gagtttgtga aaagagcgat
    46501 ttttgatatt tttttgtgag taaaatttgt aatgcttaga cgttcttatt aactcaagga
    46561 gttcgtcatg gcaaaaatat cagatttgaa ttgttatcaa cacatggcat cagccgacaa
    46621 tgttaaacca aaaaatgaag tcttaaatac ctggtgtgtg ggaatgaatg attttgctcg
    46681 taatgccgag gggcaggata gcagaaggaa tattctatct cctggagcat ttttggagtt
    46741 tttggcaaaa atatttaccc tgggttatgt ggattttagc aaatgctcca acgaagcggg
    46801 tagaaatatg atggctcata ttgagtcctc atcttatatc aaaaataatg atggcagtga
    46861 aataatgaat tttgttatga ataatcctga aggtgagcgt gcggattcat ctaaggtgat
    46921 tatagaaatt tcactttcta ctaccactac tatggggact cgtcaaggac atacagccat
    46981 tatatttcca caatctgatt ttttgagttt tcgttatgaa ggtaagtctt ttgaaagaaa
    47041 agatgatagt tcattacacc tgattactaa caaggttctg gcgtgttacc aacgtgaagc
    47101 taacaagaat atggcgcttc tattaaataa tcatcagttg aactggaagg acgcaattga
    47161 ttataacgag ttattagaaa agccattagc cagtacatta gaaaaaataa agaaagaaca
    47221 tctgctatta atgcctcatg tatgtgatga cacgattcca tacttactag gcgaaggtgg
    47281 tatacttgaa gaaataaatg atctgaaaac gttacatgat acca
//
LOCUS       DAPQZD010000016        45688 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPQZD010000016 DAPQZD010000000
VERSION     DAPQZD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.016"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..422
                     /gene="cmtB"
                     /locus_tag="RO454_000920"
     CDS             <1..422
                     /gene="cmtB"
                     /locus_tag="RO454_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDY0932792.1"
                     /translation="ESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQSIKDSTIS
                     NGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIGLSAADANS
                     HIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            450..1838
                     /gene="cmtA"
                     /locus_tag="RO454_000921"
     CDS             450..1838
                     /gene="cmtA"
                     /locus_tag="RO454_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDY0932793.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLFCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1853..3130
                     /locus_tag="RO454_000922"
     CDS             1853..3130
                     /locus_tag="RO454_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDY0932794.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3127..4092
                     /gene="yggF"
                     /locus_tag="RO454_000923"
     CDS             3127..4092
                     /gene="yggF"
                     /locus_tag="RO454_000923"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDY0932795.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4114..4623
                     /gene="fumE"
                     /locus_tag="RO454_000924"
     CDS             4114..4623
                     /gene="fumE"
                     /locus_tag="RO454_000924"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDY0932796.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4620..5333
                     /locus_tag="RO454_000925"
     CDS             4620..5333
                     /locus_tag="RO454_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDY0932797.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5305..5982)
                     /locus_tag="RO454_000926"
     CDS             complement(5305..5982)
                     /locus_tag="RO454_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDY0932798.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5976..6653)
                     /locus_tag="RO454_000927"
     CDS             complement(5976..6653)
                     /locus_tag="RO454_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDY0932799.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6641..7348)
                     /locus_tag="RO454_000928"
     CDS             complement(6641..7348)
                     /locus_tag="RO454_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDY0932800.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7349..7927)
                     /locus_tag="RO454_000929"
     CDS             complement(7349..7927)
                     /locus_tag="RO454_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDY0932801.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7950..8381)
                     /locus_tag="RO454_000930"
     CDS             complement(7950..8381)
                     /locus_tag="RO454_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDY0932802.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8753..9772
                     /gene="epd"
                     /locus_tag="RO454_000931"
     CDS             8753..9772
                     /gene="epd"
                     /locus_tag="RO454_000931"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDY0932803.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9822..10985
                     /gene="pgk"
                     /locus_tag="RO454_000932"
     CDS             9822..10985
                     /gene="pgk"
                     /locus_tag="RO454_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDY0932804.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11199..12278
                     /gene="fbaA"
                     /locus_tag="RO454_000933"
     CDS             11199..12278
                     /gene="fbaA"
                     /locus_tag="RO454_000933"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDY0932805.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12469..13329
                     /gene="mscS"
                     /locus_tag="RO454_000934"
     CDS             12469..13329
                     /gene="mscS"
                     /locus_tag="RO454_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDY0932806.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13468..14103
                     /gene="argO"
                     /locus_tag="RO454_000935"
     CDS             13468..14103
                     /gene="argO"
                     /locus_tag="RO454_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDY0932807.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14196..14936
                     /locus_tag="RO454_000936"
     CDS             14196..14936
                     /locus_tag="RO454_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDY0932808.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15104..16000
                     /locus_tag="RO454_000937"
                     /pseudo
     CDS             15104..16000
                     /locus_tag="RO454_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(15997..17052)
                     /locus_tag="RO454_000938"
     CDS             complement(15997..17052)
                     /locus_tag="RO454_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDY0932809.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(17255..18148)
                     /gene="argP"
                     /locus_tag="RO454_000939"
     CDS             complement(17255..18148)
                     /gene="argP"
                     /locus_tag="RO454_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDY0932810.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18289..18519
                     /locus_tag="RO454_000940"
     CDS             18289..18519
                     /locus_tag="RO454_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932811.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18575..19234
                     /gene="rpiA"
                     /locus_tag="RO454_000941"
     CDS             18575..19234
                     /gene="rpiA"
                     /locus_tag="RO454_000941"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDY0932812.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19490..20722
                     /gene="serA"
                     /locus_tag="RO454_000942"
     CDS             19490..20722
                     /gene="serA"
                     /locus_tag="RO454_000942"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDY0932813.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            20951..21010
                     /gene="ibsC"
                     /locus_tag="RO454_000943"
     CDS             20951..21010
                     /gene="ibsC"
                     /locus_tag="RO454_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDY0932814.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21111..21713)
                     /locus_tag="RO454_000944"
     CDS             complement(21111..21713)
                     /locus_tag="RO454_000944"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDY0932815.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21734..21917)
                     /gene="ssrS"
                     /locus_tag="RO454_000945"
     ncRNA           complement(21734..21917)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RO454_000945"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21959..22288)
                     /gene="zapA"
                     /locus_tag="RO454_000946"
     CDS             complement(21959..22288)
                     /gene="zapA"
                     /locus_tag="RO454_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDY0932816.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22456..23034
                     /gene="ygfB"
                     /locus_tag="RO454_000947"
     CDS             22456..23034
                     /gene="ygfB"
                     /locus_tag="RO454_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDY0932817.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23060..24385
                     /gene="pepP"
                     /locus_tag="RO454_000948"
     CDS             23060..24385
                     /gene="pepP"
                     /locus_tag="RO454_000948"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDY0932818.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24382..25560
                     /gene="ubiH"
                     /locus_tag="RO454_000949"
                     /gene_synonym="visB"
     CDS             24382..25560
                     /gene="ubiH"
                     /locus_tag="RO454_000949"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDY0932819.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25583..26785
                     /gene="ubiI"
                     /locus_tag="RO454_000950"
                     /gene_synonym="visC"
     CDS             25583..26785
                     /gene="ubiI"
                     /locus_tag="RO454_000950"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDY0932820.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(26859..26942)
                     /locus_tag="RO454_000951"
     CDS             complement(26859..26942)
                     /locus_tag="RO454_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932821.1"
                     /translation="MSKNTKSKIRGIRKYNAKSQVKLVYFK"
     gene            27233..28327
                     /gene="gcvT"
                     /locus_tag="RO454_000952"
     CDS             27233..28327
                     /gene="gcvT"
                     /locus_tag="RO454_000952"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDY0932822.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            28351..28740
                     /gene="gcvH"
                     /locus_tag="RO454_000953"
     CDS             28351..28740
                     /gene="gcvH"
                     /locus_tag="RO454_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDY0932823.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            28859..31732
                     /gene="gcvP"
                     /locus_tag="RO454_000954"
     CDS             28859..31732
                     /gene="gcvP"
                     /locus_tag="RO454_000954"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDY0932824.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLIRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(31898..33331)
                     /gene="bglA"
                     /locus_tag="RO454_000955"
     CDS             complement(31898..33331)
                     /gene="bglA"
                     /locus_tag="RO454_000955"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDY0932825.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            33376..33687
                     /locus_tag="RO454_000956"
     CDS             33376..33687
                     /locus_tag="RO454_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDY0932826.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            33851..34510
                     /locus_tag="RO454_000957"
     CDS             33851..34510
                     /locus_tag="RO454_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDY0932827.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(34587..35567)
                     /gene="ygfZ"
                     /locus_tag="RO454_000958"
     CDS             complement(34587..35567)
                     /gene="ygfZ"
                     /locus_tag="RO454_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDY0932828.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARTALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            35810..36076
                     /gene="sdhE"
                     /locus_tag="RO454_000959"
     CDS             35810..36076
                     /gene="sdhE"
                     /locus_tag="RO454_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDY0932829.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            36057..36464
                     /locus_tag="RO454_000960"
     CDS             36057..36464
                     /locus_tag="RO454_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDY0932830.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(36504..37025)
                     /gene="fldB"
                     /locus_tag="RO454_000961"
     CDS             complement(36504..37025)
                     /gene="fldB"
                     /locus_tag="RO454_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDY0932831.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            37137..38033
                     /gene="xerD"
                     /locus_tag="RO454_000962"
     CDS             37137..38033
                     /gene="xerD"
                     /locus_tag="RO454_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDY0932832.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            38058..38768
                     /gene="dsbC"
                     /locus_tag="RO454_000963"
     CDS             38058..38768
                     /gene="dsbC"
                     /locus_tag="RO454_000963"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDY0932833.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            38774..40507
                     /gene="recJ"
                     /locus_tag="RO454_000964"
     CDS             38774..40507
                     /gene="recJ"
                     /locus_tag="RO454_000964"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDY0932834.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            40598..41696
                     /gene="prfB"
                     /locus_tag="RO454_000965"
     CDS             join(40598..40672,40674..41696)
                     /gene="prfB"
                     /locus_tag="RO454_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDY0932835.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            41706..43223
                     /gene="lysS"
                     /locus_tag="RO454_000966"
     CDS             41706..43223
                     /gene="lysS"
                     /locus_tag="RO454_000966"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDY0932836.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(43266..43814)
                     /gene="idi"
                     /locus_tag="RO454_000967"
     CDS             complement(43266..43814)
                     /gene="idi"
                     /locus_tag="RO454_000967"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDY0932837.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            43869..43940
                     /locus_tag="RO454_000968"
     CDS             43869..43940
                     /locus_tag="RO454_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932838.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(43937..44062)
                     /locus_tag="RO454_000969"
     CDS             complement(43937..44062)
                     /locus_tag="RO454_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932839.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(44064..45512)
                     /gene="uacT"
                     /locus_tag="RO454_000970"
     CDS             complement(44064..45512)
                     /gene="uacT"
                     /locus_tag="RO454_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDY0932840.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11082 a        11269 c        12277 g        11060 t
ORIGIN      
        1 cagaatcatc aatctcagtt atacattcag caaaagactg gcaggaagct atcgatttct
       61 cgatggcatc attgctggat aaaaactata tcagcgagaa ttacattcaa tctattaaag
      121 actccaccat cagcaatggt ccttattata ttctcgcacc aggcgtggca atgcctcatg
      181 cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt gacgttactt aaacaaggcg
      241 tttatttccc ggggaaggac gaaccgatta aattactcat cgggctttct gctgccgatg
      301 ccaattcgca cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac
      361 tcgaacaact cttaacagca tcatcagaaa aacaattagc ggacattatc agccgcggat
      421 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      481 aggcttttgg gggctttttg actgcaatgg tcatccccaa tatcggtgct tttattgcct
      541 ggggatttat aacggcatta tttattccca ccggctggct gccgaatgaa catttcgcca
      601 aaattgttgg tccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc
      661 atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat tggtacgatt ggtgtgatcg
      721 tcggcgctga aatcccgatg ttccttggct cgatgattat ggggccgctc ggtggtctgg
      781 tgattaaata tatcgacaag tcgctggaaa aacgcatacc tgctggtttt gagatggtta
      841 tcaataactt ctcattaggt atcgcgggga tgctcttttg tctgctgggt tttgaagtta
      901 tcggtccggc ggtgttaatt gctaatactt tcgtcaaaga gtgtattgag gcgctggtac
      961 atgcgggtta tctgcctttg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctta
     1021 ataatgcgat tgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg
     1081 gtaaatccat cttcttcatg gtggcctcca acccaggtcc tggtctgggg ctgctgctgg
     1141 cgtttacctt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1201 acttcctcgg tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca
     1261 ttattgccat gattgcgggc ggtatgtctg gcacctggat gtttaactta ctggacggtg
     1321 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1381 gctcgttcct ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatca
     1441 cttcgctgat actgaagatg gaaaaaacgg tggaaacgaa gagcgaagat gagtttaccg
     1501 agtcagccaa tgcggttaag gcgatgaaac aagaaggtgc attctcgtta agccgtgtta
     1561 agcgtattgc ctttgtttgc gatgcaggga tgggctccag tgcgatgggc gcaaccacct
     1621 tccgtaaacg cctggaaaaa gcggggctgg caatagaagt aaaacattac gccatagaaa
     1681 acgtgcctgc ggatgcggat atcgtcgtta ctcacgccag tctggaaggg cgcgtgaaac
     1741 gtgtgacgga taaaccactg atattgatta ataactatat tggcgatcca aaactcgaca
     1801 ctttatttaa tcaattaacc gctgaacata aacactgatt acagaggtaa aaatgaaaac
     1861 caaagttgct gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga
     1921 aattactgat aatgaactgt tagtgagtgt aatttctgac agcgtttgtt tatccacctg
     1981 gaaagcggcg ttgcttggta gtgaacataa acgcgtcccc gacgatttag aaaatcatcc
     2041 ggttattacc gggcatgaat gtgctggggt tgttgtcgaa gtgggtaaaa atctcactgg
     2101 caaatataaa aaagggcagc gttttgtatt gcaaccggcg atggggctac caagcggata
     2161 ttcagccgga tacagctacg aatattttgg tggcaatgcc acctatatga ttattcccga
     2221 aatcgcgatt aatttaggct gtgtattacc gtatcacggc tcttattttg ctgcggcgtc
     2281 gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc acaccacgca
     2341 atatgtttat gagcatcgta tgggcgtcaa acctggcggc aatattgccc tgctggcgtg
     2401 tgctggcccg atgggcattg gcgctatcga ttacgccatt aacggcggca tacaaccggc
     2461 gcgggtggtg gtagtcgata tcgacgacaa acggctggcg caggtgcaga agttgctgcc
     2521 ggtggagctg gcggccagta aaggcattga gctggtgtat atgaatacca aagggatgag
     2581 cgatccggtc cagacgctgc gggcgctgac ggatgatgcc ggatttgatg acgtatttgt
     2641 ctatgcagcg gtgccttcag tcgtcgagat ggctgatgaa ttactggctg aagatggctg
     2701 cctgaacttc tttgccggtc cgacggataa aaacttcaaa gtgccgttta atttctacaa
     2761 cgtccattac aacagtacgc acattgtcgg cacgtctggc ggttccacgg acgacatgaa
     2821 agaggcgatt gcccttagcg ccacggggca gttacagccg tcgtttatgg tgacccatat
     2881 tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc ctggcggtaa
     2941 aaaactcatt tataacggcg tgaccatgcc gctgaccgcc attgccgatt ttgccgaaaa
     3001 aggcaaaacc gatccgctgt ttaaagagct ggcgcggctg gttgagaaaa cgcacggtat
     3061 ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata ttggggaggc
     3121 cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct
     3181 gcctggccgc aaaccggctg tggcgacaaa aataaaattg atggccttgc ggtcaccgcg
     3241 atgcgccagg cattaaacga cgttgctttt cgtgggcgag tggttatcgg cgagggggaa
     3301 attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga tgggccagaa
     3361 gtcgatatcg cggtcgatcc cattgaaggc acgcggatgg tggcgatggg gcagagcaat
     3421 gcgctggcgg tcatggcttt cgcccctcga gatagcctgt tacatgcgcc cgatatgtat
     3481 atgaaaaagc tggttgttaa tcgactggct gccggggcga tagatctgtc tttgcctctg
     3541 gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa gctgcgcatg
     3601 gttacgctgg ataaaccgcg cctgagtgcc gcaattgagg aagcaacaca gctcggcgtg
     3661 aaggtttttg ccctgccgga tggcgatgtc gccgccagcg tgctgacttg ctggcaggat
     3721 aatccttacg atgtgatgta caccatcggt ggcgcaccgg aaggtgttat ttctgcctgt
     3781 gccgttaaag cattaggcgg ggatatgcag gccgagctga ttgacttttg tcaggctaaa
     3841 ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg caaggcaatg
     3901 ggcgttgacg ttaaccgtgt atactcgctc gatgaactgg tgagggggaa cgatatcctc
     3961 tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca gacggcgaat
     4021 ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa tataatagac
     4081 tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga agatgatgtg
     4141 cttgagcaac tgaatgcaca ggacaattta ttttcattta tgaaaactgc gcattctatt
     4201 ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga tgaagagatc
     4261 gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga tattgatgtt
     4321 gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtacgc cgatatcacg
     4381 catttttccc agttttggca ttacctgaac gaacaggatg aaacgcccag atttgctgat
     4441 gacataacct gggattttat cagcaacgtc aatagcataa cccgcaatgc gacgctctat
     4501 gacgcgttga aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc gcgttttagc
     4561 gggatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga attaacgccg
     4621 tgaaaatcga actgacggtg aatggactaa aggtacaggc gcagtactct gatgatgaaa
     4681 ttgaaaatgt acataaacca ttgctgcgta tgctggcggc cttacagaca gtaaatccgc
     4741 agcggcgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct accctaacca
     4801 ccttctggga atatctcgcg caacaagacc cggaactgcc cgctattcag acgctgccga
     4861 tggatggttt tcaccattac aatagctggc tggatgtgca tcaattgcga cccttcaaag
     4921 gcgcaccaga gacattcaac gttgcgaaac tggcggaaaa tctgtgccgg gttgtggaag
     4981 gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa gatgtgttgc
     5041 acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgttggac gacgagaagt
     5101 ggtgccagct tgcgcaattt tgtgatttct cgatctttat caaagctccc gcatctgcgt
     5161 tgcgggagcg tctggtgggg cgcaagctgg cgggagggtt atcgctggca gacgcagaag
     5221 ccttttatga ccgtaccgac gggccgaatg ttcgccgggt gctggaagag agtctgcctg
     5281 caaatttgac cttaatgatg accgctacgg gtgaatatcg tctgatggat taacgttcct
     5341 gattacacca tcttccagtc gcaccacacg agagaaatga cgccgggtaa atgcggcgtc
     5401 atgactgatt gctacgacgc tcgtgcctcg ctggcggcac ttttccagcc agctttcaaa
     5461 cacgctcaac caattttcgt caaaatcccg actcggctcg tccagtagca atagtggcgg
     5521 agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat gcagatctaa
     5581 cggatgcgcg ctggcagtgt ctgctaactg acagcattgc agggcagcat tggtacgttg
     5641 agtgatttca tccgccgggc atttttgcag tatcaaacca aatgcgattt cgtcagcaac
     5701 ggtgctgtga aaaagctgat tttccgcttc ctgaaacagt acgccaatct ttgccgcgcg
     5761 ctggcggttt ttcaggtttt tcatcgcttg ttgttgcagc ataaccgtac cggctgtggg
     5821 cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt cccgcgccat tgtcgccggt
     5881 cagcgccagc cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgtagc
     5941 cgcgccaggc cagcgataag atatctgatt taacgttaac atagtggctg gagcgctccg
     6001 ttttgcaatt gccacgcttg ctggcagtgg ttcaggaaag gtgtgtgatg acgttcaaaa
     6061 agaataatca gcgaaccacg ttcaagcgcc cagtgttgta agcgctgcaa cagcatttcg
     6121 cgggcctgcg gcgtcaggcg gctaaaggct tcatcaagaa ttaagagctt tggctgcatc
     6181 gcgatggcgc aggcgatgac cacacgctgt gtctctccac cggagagtgt tgccgggtga
     6241 cgatggcgca acggctggca ctcggtcagg gccagggcgg cgtcaatccg cgccataatc
     6301 tctttttcag caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag
     6361 gtacagccgg agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt
     6421 tcattaagcg ggagacgccc aatgggcgta ccgagaagcg tccccgtgcc ggttatctct
     6481 cctggtagaa agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg
     6541 ccgttgtcac caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac
     6601 ggaggatgcg ttgagtgtgt cgggcaatag cgaaactgtt ctaacgtaac catatccacg
     6661 ctccgaattc agccagcatt agcaatatca tggcgtaacg tgctacgcat tgtagggtgc
     6721 tatcggctgg tgcccataac gtagtgcgcc tattgttgat tcgaaatgcc cgcatatcca
     6781 gcgccgcacc acggacagca agatcgttta gcgcattgtg tgttagcgga ataattaacg
     6841 cgggcatcgc acgtaaacgc tgataccagc cctcatccag cggcacgccg cgggcgcact
     6901 gcgcttcatg aataatcgcc aattgccgtt ttaactgctc aacgaccaat aacggcccgg
     6961 caaacagata agcgacgccc ggcggcaggc gagaagcaaa caaagcgcgg ataaaacgtt
     7021 gcacggggac gtactgcatc cacaactgtg aggttgaaac aatcgccaga atccgcaacc
     7081 agagcgtaat agcatgcgcc caccgttctg gagagtgtgg cgtgccgctt agccattctg
     7141 tcagccagcc accgtgcacc agccacagtc cagcccccag cgagaacatc agccagacga
     7201 catattttgc ccgccgccgc gtggccttta gcgcaataag gcagaaaaag gtcgccgcgc
     7261 tatagactgg caatattgtc tgtgtaggca ggataagcgt ggtgcaggcc gccagcgccc
     7321 acagcgttaa ggatgtaaaa ggatgcattt agcgcacggc agccattgcc ggaaaatggc
     7381 gcgtggtgcg aatcggcagc tgacgcagta aaagccagac tatcaccgct gtaagaattt
     7441 tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca
     7501 tcgagtgcat ccaggcaacg aacaaatcgg ctccgcttcc tgtcacgccg ccgaacagcg
     7561 cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctaacaa
     7621 cgacttttgg cagggtgcga aaccaacccg ctcgcgccag ccaaccggcg accaggccga
     7681 tgaccatcgc gacgggcgca aatgcggcgg cgatcgggtc ggtcagcaat ccccagagta
     7741 aattggtcag taaaccggtc agcatcccga ttatcggacc aagcagtacc gcgctaataa
     7801 gtgtgccgat ggagtcgaga aaaataggta atttaaccat gctggccagt tgcccgccga
     7861 tcatattgat ggcgatagag ataacgatca acaccagtgc ctgactggaa aagtggcgac
     7921 gcgccataag aaaacctctc tgaaaatagt tatttaacgg gggaaatatg caactcgtcg
     7981 taaccagaaa gcgcgcaaag ctggcggcta aacgccagtt cctcaaggca gccgatcacc
     8041 agttcaagcg tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg
     8101 tgtggatcgg aaacgcagag atgaagatgt tcaccactct gttccagcgt gccgttcaat
     8161 gcgatgactt cgaacttacc gcttagcagg gcagtgtttt cttgcccggc atagcgtaag
     8221 gcgacgtcag tcagactgcc ggtacaacct gcaatccacg cggcgtgaag ttgttgttgc
     8281 tgcgcgaagg cgcgtaattg ggagagaact tcctgcccgg ggagcaggcg cagcgcgtaa
     8341 aagcgtgcgg aggaagaata tgggtagggc aatgtcatca ttggagtact ccgaaactcg
     8401 gacgtattgc ggataatatc gacgctgcat cagtcatatt ctgatataag tcagggtttg
     8461 tgaccgcaca acgaaaagat attgctacgc ttattcgtga ttctgcaaag tggaataaat
     8521 tccacagggg cggggatgaa aacgtgacat aagtcagata attgtcttct ttgactggac
     8581 aaacattcct tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat
     8641 gttcgacaat taaccaatca gtcgcagttt gcgacaggta aggtttcccc gaacgatttg
     8701 ctggattact ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt
     8761 acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga
     8821 atccggacgc cgtgcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg
     8881 catggcgcat ttgttgaaat atgacaccag ccatggccgt tttgcatggg aagtacgaca
     8941 ggaacgcgat caactttttg ttggtgatga cgccatccgt gtattgcatg aacgttcact
     9001 gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata
     9061 tggctcccgc gagcatggtg aagcacacat tgccgccggg gctaaaaaag tgctcttttc
     9121 acatcctggc agtaacgatc tcgacgcgac cgttgtttat ggcgtcaatc aggatcaact
     9181 tcgtgcggaa caccgcatcg tttctaatgc ttcctgtacc acgaattgca taattcccgt
     9241 catcaaattg ttagatgatg cgtacggtat tgagtctggc actgtgacca caattcactc
     9301 cgccatgcat gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcactcgggc
     9361 agccagtcag tcgatcattc cggtcgatac taaactggcc gccggtatca cacgattttt
     9421 tccgcaattt aacgatcgct ttgaggcgat tgcggtacgt gtgccaacca taaatgtgac
     9481 ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt
     9541 gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt
     9601 ggtctctgta gattttaacc acgatccgca cagtgccatt gtcgatggca ctcaaacccg
     9661 ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc
     9721 taaccgaatg ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag
     9781 cagcgtctac aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga
     9841 ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa
     9901 aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg
     9961 ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg
    10021 agtacaacga agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta
    10081 acccggttcg tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg
    10141 ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa actctgtcca
    10201 aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg
    10261 cgcaggcttc tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc
    10321 tggcagctga actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg
    10381 ctatcgttgg tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa
    10441 tcgctgacca gctgatcgtt ggtggtggta ttgctaacac ctttattgcg gcacaaggcc
    10501 acgatgtggg taaatccctg tacgaagctg acctggttga cgaagctaaa cgtctgctga
    10561 ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaactg
    10621 caccggctac cctgaaatct gttaacgatg tgaaagctga cgagcagatc ctggatatcg
    10681 gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga
    10741 acggtccggt tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta
    10801 acgctatcgc agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa
    10861 tcgacctgtt cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc
    10921 tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga
    10981 agtaaaaaat cacagggcag ggaaacctgc ccttgtttca gcgcgctttt agagcacgca
    11041 ctttttcata ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatcccagga
    11101 gcttacataa gtaagtgatt ggggtgagcg aacgcagacg cagcacatgc aacttgaagt
    11161 atgacgagta taaggcccga cgatacagga caagagacat gtctaagatt tttgatttcg
    11221 taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta gcaaaagaaa
    11281 acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac gccgtgctgg
    11341 aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt ggtgcttcct
    11401 ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc ctgggcgcga
    11461 tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg gttatcctgc
    11521 acaccgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg gacgcgggtg
    11581 aaaaacactt cgcagctacc ggcaagccgc tgttctcttc tcacatgatc gacctgtctg
    11641 aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgc atgtccaaaa
    11701 tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac ggcgtggaca
    11761 acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat tatgcgtata
    11821 ccgagctgag caaaatcagc ccgcgtttca ccatcgcagc gtccttcggt aacgtacacg
    11881 gtgtttacaa gccgggtaac gttgttctga ctccgaccat cctgcgcgac tctcaggaat
    11941 atgtttctaa gaaacacaac ctgccgcaca acagcctgaa cttcgtattc cacggtggtt
    12001 ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg aaaatgaaca
    12061 tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac aaagcgaacg
    12121 aagcttacct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg aacaagaaat
    12181 actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct cgtctggaaa
    12241 aagcattcca ggaactgaac gcgatcgacg ttctgtaagt cattcttttc tgcttatctc
    12301 aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg tgatgcagat gagaaatctg
    12361 tgatctattt ggcaaaatta tgctttattg tttacccttg tcagactgcc cgccataagg
    12421 cggcggagtg tatttctcca ttttgagtca gttgaaaagg aatattgaat ggaagatttg
    12481 aatgttgtcg atagcataaa cggcgcggga agctggctgg tggctaacca ggcgctgctg
    12541 ctaagttatg cagtaaacat cgtggcggca ctcgcgatca tcatcgttgg tttgattatc
    12601 gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact
    12661 gttgctgatt ttctttctgc attagtccgt tacggaatta tcgcctttac gctaatcgct
    12721 gcactggggc gcgtgggcgt acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc
    12781 ttagctgttg gtctggcttt gcaggggtca ctgtctaacc tggccgctgg cgtgttactt
    12841 gtcatgttcc gcccgttccg tgccggtgaa tatgttgacc tcggcggcgt agccggcact
    12901 gtgctgagtg tgcagatttt ctccaccacc atgcgtactg cagacggtaa aattatcgtt
    12961 attccgaatg gtaaaattat tgccgggaat attattaact tctcccgcga gccagctcgc
    13021 cgtaacgaat ttattattgg cgtggcgtat gattccgata tcgatcaggt taagcagatc
    13081 ctgaccgata ttatccagtc tgaagatcgc attttgaaag atcgcgaaat gactgtgcgt
    13141 ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc gagtctggag caacagcggc
    13201 gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc
    13261 ggtatcagct tcccgtatcc gcaaatggat gtgaacttta agcgggtgaa agaagacaaa
    13321 gctgcgtaat caacgctgag gccagataat actctctggc ctctctctta ttagtttttc
    13381 tgattgccaa ttaatattat caatttccgc taataacaat cccgcgatat agtctctgca
    13441 tcagatactt aattcggaat atctaacgtg ttttcttatt actttcaagg tcttgcactt
    13501 ggggcggcta tgatcctacc gctcggtcca caaaatgctt ttgtgatgaa tcagggcatt
    13561 cgtcgtcagt accacattat gattgcctta ctttgtgcta tcagcgattt ggtcctgatt
    13621 tgtgcgggga tttttggtgg cagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg
    13681 gtcacctggg gcggcgtagc cttcttgctg tggtatggtt ttggcgcttt taaaacagca
    13741 atgagcagta acattgagtt agccagtgcc gaagtcctga aacaaggcag atggaaaatt
    13801 atcgccacca tgttggcagt gacctggctg aatccgcatg tttacctgga tacttttgtt
    13861 gtgctgggca gccttggcgg gcaacttgat gtggaaccaa aacgttggtt tgcgctcggg
    13921 acaattagcg cctctttcct gtggttcttt ggtctggcta ttctcgcagc ctggctggca
    13981 ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc tggtagtggg atgtgttatg
    14041 tggtttattg ccttgcagct ggcgagagac ggtattgctc atgcacaagc cttgttcagt
    14101 taggcgctat ctgatggaaa aataaaacag aggcgctaag cttgcctcca gaggtcctga
    14161 attttgggca agtcaattaa cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg
    14221 cggcattaat gggtattagc gggatggcag cgcaggctaa cgaattgccg gatggaccgc
    14281 atattgtcac ctccggtacg gcaagcgtgg atgcggtgcc agacattgcc actcttgcga
    14341 ttgaagttaa cgtggccgcg aaggatgccg ctactgccaa gaaacaggca gatgagcgcg
    14401 tcgcacaata catttccttc cttgagctca atcagatcgc gaaaaaagat atcagctcag
    14461 cgaacttacg cactcagcca gattatgatt atcaggatgg taaaagtatc ctgaaaggct
    14521 accgcgcagt gagaacggtg gaagtcacgc tccgtcagtt agacaaactg aattccttgc
    14581 tggacggcgc gctaaaggcg ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc
    14641 agccggatgc ctataaagac aaagcgcgta aggcagcgat tgataacgcg attcatcagg
    14701 cacaggaact ggcgaatggc tttaatcgta aactggggcc ggtatatagc gtgcgctacc
    14761 atgtttccaa ctatcagccc agcccaatgg tgcggatgat gaaagctgat gctgcaccgg
    14821 tgtccgcaca ggaaacttac gagcaggccg ctattcagtt tgatgatcag gtcgatgtag
    14881 tcttccagtt agaacctgtg gatcaacaac ctgctaaaac acctgcagca caataatact
    14941 tgtagcccga cgtattcgtc gggctaatgc ctttacccga tattgcctcc tttttctgta
    15001 attttctgta aaaccatgac gtaatcacct atctttgctc gcggtttcat caaggatagg
    15061 ctgactgaaa cggttaagcg gacaaggatg cgggagaggg gggatggaca tttttatttc
    15121 gaaaaagatg cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga
    15181 aaaaatccat atgacggctt cgccatttgg caaaagcatt gccgcgctgg aagaacaaat
    15241 tggctatacg ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggacagga
    15301 actgtatcaa aaactgttcc cggtttatca acgactttct gcgattgata atgaaatcca
    15361 taactcagga cgtcgttaac gagaaattgt gataggcata gataatactt atccgacaat
    15421 tatttttgat cagttgatta gtctcggaga taagtacgaa ggcgtgacca tccagccagt
    15481 tgagttcagc gaaaatggcg ttatcgataa tctttttgac cgtcagttag attttattat
    15541 ctccccacag catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaact
    15601 accaccgctg cgattgggct ttctcgtttc tcgccgttat gaagggcgac aggagcaaga
    15661 gctgcttcag gagctgccct agctacaaat gcgctttcaa aaccgggcta acttcgaagc
    15721 aatgattgat gcgaatatgc gtccttgtgg gatcaatcca accattattt atcgtcccta
    15781 tagttttatg gctaaaatca gcgccgtgga gcggggacat tttctgacgg tgatcccgca
    15841 ttttgcctgg cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc
    15901 gatgtatatg caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatgct
    15961 tcagcatatt gctgaagatc gtgacgggac aaaccattaa cccagcatcg atccggttgc
    16021 aattaaatta cggtgtaagt cgaagacgtg gctaagatcg tggtgaatat gtccgccgga
    16081 cgcattttcc agatatcgat gcagataatc ccgatacatg ggatgtgcac aatgatcaat
    16141 ggtggtgcgg ataacgttgt tatacggatc ctgtgcgcca aatccactga ccgcttcgct
    16201 ccataaataa cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccaatgcc
    16261 gaagaaaaac gacaggcgag gggcgaagtc atcaattttc agcccggcgg agattgctgc
    16321 tttgatgtac tcaatcctat cggcgagcgt aaatgctacc tgctgcacgc agttggcacc
    16381 cgcttcaccc atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga
    16441 acaccaggcg atgatgtctg cgataatacg cattgacggt tttggtgggt aaatataggt
    16501 gttgcggcaa agatactctt tgaggatatc gttttgaatg gtgccggtca gtttatcgga
    16561 tgtaacacct tgctcttctg cggcgacgat ataaaacgcc agtactggta gcactgcgcc
    16621 attcatggtc atcgaaaccg acattttatc cagcgggatc tggtcgaaca ggacttttat
    16681 atcttccacg gtgtcgatag cgacgcccgc tttgccgacg tcgcccgcca cgcgtgggtt
    16741 atcggagtcg tagcctcggt gggtggccag gttacggcga taaaaagcgt tggactcttt
    16801 tgctgtcgag aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    16861 acgcgggcca cgaacgtagg ggggcaaacc aggaagggta cccgtcacct ccagattatc
    16921 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    16981 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    17041 cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    17101 tgagcttttt acgtgggttt gtcgccagca aaaaaatgca ttcagacgtc gttttttgcc
    17161 gcctgagatt tgtctatttt gcgatgtgat tcaaggaata caaaaagaag tgaggataac
    17221 gcctgatatg tgctatcagg cgttatttga tggattaatc ctgacgaaga actttgtgac
    17281 cataatcgag caacgcatca gtgactttac gcatcatgcg gctttcagga gcaaagcggt
    17341 gccagtagag catccggcgt tgaaatagcc ctggcgttaa gtcaatcagt tcgccgctgg
    17401 ccagctcttt ctcgatttgc aggtgcggga tcatacagca ggtggtgccc tgacgagcaa
    17461 gttgcacgaa cgcttccgaa gaattaacga tatggcaggg tacgctgcca ggaggcagat
    17521 cgaaattttg ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac gcgaccactg
    17581 gcgctttcag taatgccgaa cgcgttacgc cgttagggaa atatttttcg gcaaatggtt
    17641 ttgagctgac gaacagatag tcgagcgcac cgagtttatc gacaagacaa ctcggcagcg
    17701 cctgatgttg aatactcact gcgccgacca cttcgccgcg acgcagacgt tcctgagtgc
    17761 gggtttcatc ttctacctgc aagttgaggc ggatgggcga atcagccaac acaggagcca
    17821 gtgccggaag caaccacgtc gccagactgt cggcgttgac cgccagtgaa agcagcagcg
    17881 gagtcgaacc ggtttgttca tcgcccagcc actcttcttc cagcagctcc acctggcgta
    17941 gtagcgccaa cagtttttgc ccttgttccg tcgggcgcgg cggtacggta cgcactaaca
    18001 acggctgccc gaacatattt tccagttgct taatgcgctg tgagacggct gattgtgtaa
    18061 tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca tccagtgcct
    18121 gtaatgttct gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa atgctttttc
    18181 ctgcactatg acacaatttt gtgtcgggtt gcatataccg aagccgttgg cgggtaacat
    18241 ttgtatcagt ggttaacgcg cagcttaaaa gatcagagat ttgcgctaat gcatttcgct
    18301 cagcgagttc gcgccctggt tgtgttgaat ggcgtggcgt tattgcctca gtttgcctgt
    18361 aaacaggggc ttgcgaacgg ggaactggtg cgcctgttcg caccgtggag cggcataccc
    18421 agaccgttgt atgctttatt tgcggggcga aaagggatgc ccgccattgc gcgatatttt
    18481 atggatgagt taaccacgcg gcttgccaac ggggtctgaa tcgctttttt tgtatataat
    18541 gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc
    18601 agtaggatgg gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg
    18661 ttccaccgcc gcacacttta ttgacgcact cggtacaatg aaaggccaga ttgaaggggc
    18721 cgtttccagt tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga
    18781 tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca
    18841 catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt
    18901 tgcggaaaaa tttatctgta ttgcagacgc ttccaagcag gttgatattc tgggcaaatt
    18961 cccgctgcca gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa
    19021 actgggcggt cgtccggaat accgtcaggg cgtagtgacc gataacggca acgtgatcct
    19081 cgacgtccac ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat
    19141 tcctggcgtg gtgactgttg gtttgtttgc taaccgtggc gcggacgttg cgctgattgg
    19201 cacacctgac ggtgtcaaaa ctattgtgaa atgatctgac gggggaacct cccccgttaa
    19261 aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt tttaccaggc aattgtcgat
    19321 tgctctaaat aaatcctcta aaccagcata ttcatccaag aaatactttt gcgtgatatt
    19381 tcctcaacat cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa
    19441 aggcggatgc aaatccgcac acaacatttc aaaagacagg attgggtaaa tggcaaaggt
    19501 atcgctggag aaagacaaga ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct
    19561 ggaaagcctt cgtgcagctg gttacaccaa catcgaattt cacaaaggcg cgctggatga
    19621 tgaacaatta aaagaatcca tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca
    19681 tctgactgaa gacgtgatca acgccgcaga aaaactggtc gctattggct gtttctgtat
    19741 cggaacaaac caggttgatc tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc
    19801 accgttctca aatacgcgct ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt
    19861 gcgcggcgtg ccggaagcca atgctaaagc gcatcgtggc gtgtggaaca aactggcggc
    19921 gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc ggctacggtc atattggtac
    19981 gcaattgggc attctggctg aatcgctggg aatgtatgtt tacttttatg atattgaaaa
    20041 caaactgccg ctgggcaacg ccactcaggt acagcatctt tctgacctgc tgaatatgag
    20101 cgatgtggtg agtctgcatg taccagagaa tccgtcaacc aaaaatatga tgggcgcgaa
    20161 agagatttcg ctaatgaaac ccggctcgct gctgattaat gcttcgcgcg gtactgtggt
    20221 ggatattcca gcgctgtgtg acgcgctggc gagcaaacat ctggcggggg cggcaatcga
    20281 cgtattcccg acggaaccgg cgaccaatag cgatccattt acctctccgc tgtgtgaatt
    20341 cgacaatgtc cttctgacgc cacacattgg cggttcgact caggaagcgc aggagaatat
    20401 tggcctggaa gttgcgggta aattgatcaa gtattctgac aatggttcaa cgctctctgc
    20461 ggtgaacttc ccggaagtct cgctgccact gcacggtggt cgtcgtctga tgcacatcca
    20521 cgaaaaccgt ccgggtgtgc tgactgcgct caacaaaatt tttgccgagc agggcgtcaa
    20581 catcgccgcg caatatctgc aaacttccgc ccagatgggt tatgtagtta ttgatattga
    20641 agccgacgaa gacgttgccg aaaaagcgct gcaggcaatg aaagctattc cgggtaccat
    20701 tcgcgcccgt ctgctatact aaatcccttt ctctgaaaat caacgggcag gtcactgact
    20761 tgcccgttat tttatccctt ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt
    20821 ctgctccaga aaataaatca aagtcagatc aatgcgttgt gtttatttaa tggggattta
    20881 cgatggcggg actgtacagg gctgtaacgg taaagccctc accgaagcga gggcttgaag
    20941 gagaagggtt atgatgcgac ttgtcatcat actgattgta ctgttactca taagtttcag
    21001 cgcttattaa cagtcagtca caggggagga gcaatccccc cttaccctta ctcactaaat
    21061 taggttaaag aatcaacgat gtcaatcagg gcgatgcagg tgtgtcgccc tcaccactcc
    21121 cagactttcg acggtgtaac caccgcaggc agtgggatat cccactcttc aacggggagt
    21181 ttttccacca actgacaatc atgcgcataa cccaccggtt gcattttata gtgctgccag
    21241 ttttgtaagg tccgatcata aaaaccaccg cccattccca ggcgctgacc gtactcatca
    21301 aaggcgacca gcggtgtaat caacacgtct aatcgggaaa ggggtagcac gtcacgcaca
    21361 tccagttttg gctcatggat cttcaacttg ttcatcacca gttcgctttg tggatggtaa
    21421 ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa ctggaagata tacgcgctta
    21481 ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga gttcgccatc aaaagagagg
    21541 aatacagcga ccgtatgtgc catcaccacc gggggataag tcatcatccg ggtagcggct
    21601 tgttgaccca tttcctgctg ttgttccggc gttaacgcac gacgacgttg ccgaatcatt
    21661 ttgcggattt cttgtcggga taatgtcagt gggagttctg gtaattgcgt catggctggt
    21721 gccagataag aagggaatct ccgagatgcc gccgcaggct gtaacccttg aacccttggt
    21781 tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc atgctcacca
    21841 accgcggagc gccacattct tgtggtatga aatatcggct caggggactg gcccgcttgc
    21901 gaacatctca gagaaatttt gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc
    21961 attcaaagtt ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta
    22021 tggtctgctg cagcatccga atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg
    22081 ctttttcttg cgctaactca tagctgatat tcagtgcggc aatgaagacc aactgttcag
    22141 tatttgtgac tctagtgcgt tctttcagat cttgcaaccg ttggttcaga tcgtccgctg
    22201 cctgattcaa cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa
    22261 tttggatatc gacgggttgt gcagacatgc caccttcctg ctgattgact gcgctgcctt
    22321 cgtctttaga cccatggtct gcgaaggggc gacactatag ctaccctgat gagaagagac
    22381 aagccctttt ctggtccacc agggcccaaa gtggtagcat atcatgaata ttcctccctt
    22441 tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg gttacaacga aatgaaccag
    22501 tatctgaacc aacaagggac gggtctgacc cctgctgaga tgcatggttt aatcagtggg
    22561 atgatatgtg gcggtaacga tgacagctca tggctgccgc tacttcacga cctgacgaac
    22621 gaaggcatgg ctttcggtca tgagctggca caggcactgc gcaaaatgca ctctgccacc
    22681 agcgatgccc tgcaggatga cggcttcctt tttcagcttt atctacctga tggcgatgat
    22741 gtcagcgttt tcgatcgggc tgatgcgctg gctggttggg tcaatcactt cctgcttggt
    22801 cttggcgtta cgcaaccgaa gctggacaaa gtgaccggcg aaaccggtga agccatcgac
    22861 gatctgcgta acatcgcgca gttgggttac gacgaagacg aagatcagga agagcttgaa
    22921 atgtcgcttg aagagatcat cgagtacgtc cgtgttgccg cgctgttatg ccacgacacc
    22981 tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa
    23041 aaaacgtaag gagagtgtta tgagtgatat atcccggcaa gaatttcagc gtcgccgtca
    23101 ggccctggta gagcaaatgc aacccggcag cgccgcgctg atttttgctg caccagaagt
    23161 aacacgtagc gccgacagcg aataccccta tcgtcagaac agtgacttct ggtacttcac
    23221 cggctttaac gaaccggaag cggtgctggt gctgattaaa agcgatgaca ctcataacca
    23281 cagcgttttg tttaaccgcg ttcgcgacct gacggcagag atctggtttg gtcgtcgctt
    23341 aggccaggat gccgcgccag agaaactggg cgttgaccgc gcactggcat tcagcgaaat
    23401 caatcaacaa ctttatcaac tacttaatgg tctggatgtg gtttaccacg cccagggcga
    23461 atatgcatat gctgatgaaa tcgtgaacag cgcgcttgag aaactgcgta aaggttcgcg
    23521 gcaaaatctc accgcaccgg caacgatgat cgactggcgt cctgttgttc atgaaatgcg
    23581 cctgttcaaa tcaccagaag agattgccgt acttcgccgc gcgggtgaaa tcaccgcact
    23641 ggcgcataca cgggcgatgg aaaaatgccg tccggggatg ttcgagtacc atctggaagg
    23701 cgaaattcac cacgaattta accgccacgg tgcgcgttac ccttcctata acaccattgt
    23761 tggcagcggt gaaaacggct gcattttgca ttacaccgaa aacgagtgtg aactgcgtga
    23821 cggtgacctg gtgttgattg acgcgggttg tgaatacaaa ggttacgctg gcgacattac
    23881 ccgcaccttc ccggtcaacg gcaaattcac tcaggctcag cgtgaaatct atgacattgt
    23941 gctggagtct cttgaaacca gcctgcgcct gtatcgtccg ggaacttcca ttcaggaagt
    24001 cactggtgaa gtggtgcgca tcatggttag cggtctggta aaactcggca tcctgaaagg
    24061 tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct ttctttatgc atggccttag
    24121 ccactggtta ggactggatg ttcatgacgt tggtgtttat ggtcaggatc gctcgcgtat
    24181 tctggaaccg ggtatggtac tgaccgtaga accaggactg tatattgcgc cggatgcaga
    24241 agtgccagaa cagtatcgcg gtatcggcat tcgtattgaa gacgatattg tgattaccga
    24301 aaccggtaat gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt
    24361 gatggcagct gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg
    24421 acgctggcgc tggctatttc ccggttaagt cacggggcgc tgccagtaca tttgattgaa
    24481 gcgaccgcgc cagagtcaca tgcgcatccg ggctttgatg gacgagctat tgcgctggcg
    24541 gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc tgattgcgca
    24601 actgccatca ccaccgtgca tgttagcgat cgtggtcacg ctggatttgt caccctcgcc
    24661 gcagaagatt accaactggc ggcgctgggg caggttgtcg aattgcacaa tgtcgggcaa
    24721 cggctgtttg cattgctgcg caaagcacct ggcgtaacgc tgcattgccc tgatcgcgtg
    24781 gctaacgttg cccgtactca gagccacgtt gaagtgacgc tggagagtgg cgagacgctg
    24841 acgggccgcg tgctggtagc cgctgatggc acccattcag cgttagccac cgcgtgcggt
    24901 gttgactggc agcaggagcc ttacgaacaa ctggctgtga ttgccaacgt tgcgacttcc
    24961 gttgcgcatg aagggcgcgc ttttgaacgc tttacacaat atggcccgct ggcgatgttg
    25021 ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag
    25081 gtgctgtcgt ggagcgacga gaagttctgc cgtgaactcc agtcggcctt tggctggcga
    25141 ctggggcaaa ttacccacgc cggaaaacgt agtgcttatc cgctggcgtt aacccgcgcc
    25201 gccaaaccta ttacccatcg taccgtgctg gtggggaatg cggcgcaaac tctgcatccc
    25261 atcgccgggc aaggatttaa cctcggtatg cgtgatgtga tgagtcttgc agaaaccctg
    25321 actcaggcgc aggaacgcag agaagacatg ggtgattacg gcgtattgtg ccgttatcag
    25381 cagcgtcgac agcgcgatcg cgaagcaacc atcggcgtca cggacagcct tgtacatctt
    25441 tttgccaacc gttggacgcc gctggttgtc gggcgcaaca tcgggctgat gacgatggaa
    25501 ttatttaccc cggcacgcga tgtgctggcg cagcgcactc ttggttgggt ggcgcgttga
    25561 aggcggtttt aaggaataaa caatgcaaag tgttgatgta gccattgttg gcggcggcat
    25621 ggtcgggctg gcggttgcct gcggcttaca gggtatcggc ttacgcgttg ccgtactgga
    25681 gcagcgcgta ccggaacctc tggcggcgga tgcaccacca caactgcgcg tttcggctat
    25741 caatgccgcc agcgaaaaat tactcacccg tcttggcgtc tggcaggaca ttctctctcg
    25801 tagggcctgt tgctatcacg gcatggaagt gtgggacaaa gacagctttg gtcacatctc
    25861 gtttgacgat caaagcatgg gctatagcca tcttgggcat atcgttgaaa actcagtgat
    25921 tcactacgcg ctgtggaaca aagcgctgca gtcgtcagat atcactctgt tggcccccgc
    25981 agaattacag caggtcgcct ggggtgaaaa tgaaaccttc ctgacgttga aagatggcag
    26041 catgttaacg gcgcgtctgg tgattggcgc ggacggcgct aattcctggt tgcgcaacaa
    26101 agccgatatt ccgctgactt tctgggatta tcagcatcac gcgctggtgg cgaccattcg
    26161 cacggaagaa ccgcatgatg cggtggcgcg acaggttttt catggcgaag gcattctggc
    26221 ctttttgccg cttagcgatc cgcatctttg ctcgattgtc tggtcactgt cgccagagga
    26281 agcgcagcgg atgcagcagg caggcgaaga cgaatttaat cgcgcgttaa atatcgcttt
    26341 tgataatcgc ctgggcttat gcaaggttga aagtgagcgc ctggtgttcc cgctaacggg
    26401 gcgttatgcg cgccagtttg ccgcgcaccg tctggcgctg gtgggcgacg ccgcgcatac
    26461 cattcacccg ttggcggggc agggggtaaa tctcggcttt atggatgctg cagagctgat
    26521 tgctgaactg aaacggttgc atcgtcaggg taaagacatc gggcagtaca tttacctgcg
    26581 tcgctatgag cgtagccgca agcacagcgc ggcgctgatg ctggctggta tgcagggatt
    26641 ccgcgatctg ttttccgggg ctaatccggc gaaaaaattg ctgcgtgata ttggtctgaa
    26701 actggctgat acgcttcctg gcgttaagcc acaacttatt cgccaggcaa tgggattaaa
    26761 cgatttgcct gaatggctgc gttaaaaatt tctcttctgc tgtttatttg atacccatca
    26821 cactttcatc tcccggtttt ttcgccggga gattttcctc atttgaaata aactaatttc
    26881 acctgcgatt tcgcattata ttttctaatg ccacgaattt ttgatttagt gttttttgac
    26941 atttttttag ttcttaatgt tgtcttattc aaattaactt tctcatctat ttatctttat
    27001 atagagattt gttcattttt tggtttttta ttctgttacg attttggcgt tttttaacca
    27061 taagctaatg tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc
    27121 aggcaaaaga gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt
    27181 gataatttcg ccatagaaaa gttgtcagcc ccgcttattc aatgaggaca agatggcaca
    27241 acagactcct ttgtacgaac aacacacgct ttgcggcgct cgcatggtgg atttccacgg
    27301 ctggatgatg ccgctgcatt acggttcgca aatcgacgaa catcatgcgg tacgtaccga
    27361 tgccggaatg tttgatgtgt cacatatgac catcgtcgat cttcgcggca gccgcacccg
    27421 ggagtttcta cgttatctgc tggcgaacga tgtggcgaag ctcaccaaaa gcggcaaagc
    27481 cctttactcg gggatgttga atgcctctgg cggtgtgata gatgatctca tcgtctacta
    27541 ctttactgaa gatttcttcc gcctcgttgt taactccgcc acccgcgaaa aagacctctc
    27601 ctggattacc caacacgctg aacctttcgg catcgaaatt actgttcgtg atgacctttc
    27661 catgatcgcc gtacaagggc cgaatgcgca ggcaaaagct gccacactgt ttaatgacgc
    27721 ccagcgtcag gcggtggaag ggatgaaacc gttctttggc gtgcaggcgg gcgatctgtt
    27781 tattgccacc accggttaca ccggtgaagc gggctacgaa attgcgctgc ccaatgaaaa
    27841 agcggccgat ttctggcgcg cgctggtgga agctggcgtt aagccatgtg gcctgggcgc
    27901 gcgtgacacg ctgcgtctgg aagcgggcat gaatttgtat agccaggaga tggacgaaac
    27961 tatttctcct ttagccgcca atatgggctg gactatcgcc tgggaaccgg cagatcgtga
    28021 ctttatcggt cgtgaagctc tggaagcgca gcgtgaacat ggcacagaaa aactggttgg
    28081 tctggtgatg accgaaaaag gcgtgctgcg taatgaactg ccggtacgtt ttactgatgc
    28141 gcagggcaac cagcatgaag gcattatcac cagcggtact ttctccccaa cgctgggtta
    28201 tagcattgcg ctggcgcgcg tgccggaagg tattggtgaa acggcgattg tgcaaattcg
    28261 caaccgtgaa atgccggtta aagtgacgaa acctgttttt gtgcgtaacg gcaaagccgt
    28321 cgcgtgattt acttttttgg agattgattg atgagcaacg taccagcaga actgaaatac
    28381 agcaaagaac acgaatggtt gcgtaaagaa gccgacggca cttacaccgt tggcatcacc
    28441 gaacacgctc aggagctgtt aggcgatatg gtgtttgttg acctgccgga agtgggcgca
    28501 acggttagcg cgggcgatga ctgcgcggtt gccgaatcgg taaaagcggc gtcagatatt
    28561 tatgcgccag taagcggtga aatcgtggcg gtaaacgatg cactgagcga ttctccggaa
    28621 ctggtcaaca gcgaaccgta tgcaggcggc tggattttca aaatcaaagc cagcgatgaa
    28681 agcgaactgg aatcactgct ggatgcaacc gcatacgaag cattgttaga agacgagtaa
    28741 cggctttatt cctcttctgc gggagaggat cagggtgagg aacatttacg cctcaccctc
    28801 actctcttcg taaggagaga ggttcacaat tcactgcacg tttcaggaac catcgctcat
    28861 gacacagacg ttaagccagc ttgaaaacag cggcgctttt attgaacgcc atatcggacc
    28921 ggacgccgcg caacagcaag aaatgctgaa tgccgttggc gcacaatcgt taaacgcgct
    28981 gaccggccag attgtgccga aagatattca gcttgcgact ccgccgcagg ttggcgcacc
    29041 ggcgaccgaa tacgccgcac tggcagaact caaggctatt gccagtcgca ataaacgctt
    29101 cacgtcttac atcggcatgg gttacaccgc cgtgcagcta ccgccggtta tcctgcgtaa
    29161 catgctggaa aatccgggct ggtataccgc gtatactccg tatcaacctg aagtctccca
    29221 gggccgcctt gaagcactgc tcaacttcca gcaggtaacg ctggatttaa ccgggctgga
    29281 tatggcctct gcttctcttc ttgacgaagc caccgctgcc gccgaagcaa tggcgatggc
    29341 gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttt gttgcttctg acgttcatcc
    29401 gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt ggttttgaag tgattgtcga
    29461 tgatgcgcaa aaagtgctcg accatcagga cgtcttcggc gcgctgttac agcaggtcgg
    29521 cactactggt gaaattcacg actacactgc gcttattagc gaactgaaat cacgcaaaat
    29581 tgtggtcagc gttgccgccg atattatggc gctggtgctg ttaactgcgc caggtaaaca
    29641 gggcgcggat attgtttttg gttcggcgca acgctttggc gtaccgatgg gctacggtgg
    29701 cccacacgcg gcattctttg cggcgaaaga cgaatacaaa cgctcaatgc cgggccgtat
    29761 tatcggtgta tcgaaagatg cagctggcaa caccgctctg cgcatggcga tgcagactcg
    29821 tgagcaacat attcgtcgtg aaaaagcgaa ctccaacatt tgtacatccc aggtcttgct
    29881 ggcaaacatt gccagtctgt atgccgttta ccacggtccg gttggcctga aacgtatcgc
    29941 taaccgcatc caccgtctga ccgatatcct ggcggcgggc ctgcaacaaa aaggtctgaa
    30001 gctgcgccat gcgcactatt tcgatacttt gtgtgtggaa gtggccgaca aagcgggcgt
    30061 gctggcgcgt gccgaagcgg ctgaaatcaa cctgcgtagc gatattctga acgcggttgg
    30121 gatcaccctt gatgaaacca ctacgcgcga aaacgtgatg cagcttttca gcgtgctgtt
    30181 gggcgataac cacgggctgg acatcgacac actggacaaa gacgtggctc acgacagtcg
    30241 ctctattcag gctgcgatgc tgcgcgacga cgaaatcctc acccatccgg tgtttaatcg
    30301 ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg ctggagcgta aagatctggc
    30361 gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg aaactgaacg ccgccgccga
    30421 gatgatcccg atcacctggc cggaatttgc cgaactgcac ccgttctgcc cgccggaaca
    30481 ggctgaaggt tatcagcaga tgatcgcgca gctggctgag tggctggtga aactgaccgg
    30541 ttacgacgcc gtttgtatgc agccgaactc tggcgcacag ggcgaatacg cgggcctgct
    30601 ggcgattcgt cattatcatg aaagccgcaa cgaagggcat cgcgatatct gcctgatccc
    30661 ggcttctgcg cacggcacta acccggcttc cgcgcatatg gcaggaatgc aggtggtggt
    30721 tgtggcgtgt gataaaaacg gcaacatcga tctgactgat ctgcgcgcga aagccgaaca
    30781 ggcgggcgat aacctctcct gtatcatggt gacctatcct tccacccacg gcgtgtatga
    30841 agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc ggcggtcagg tttaccttga
    30901 tggcgcgaac atgaacgctc aggttggcat cacctcgccg ggctttattg gtgcggacgt
    30961 ttcccacctt aacctgcata aaactttctg cattccgcat ggcggtggtg gtccgggtat
    31021 gggaccgatc ggtgtgaaag cgcatctggc tccgtttgta ccgggtcata gcgtggtgca
    31081 aatcgaaggc atgttaatcc gtcagggcgc ggtttctgcg gcaccgttcg gtagtgcctc
    31141 tatcctgcca atcagctgga tgtacatccg catgatgggc gcagaagggc tgaaaaaagc
    31201 aagccaggtg gcaattctta acgccaacta tattgccagc cgtctgcagg atgccttccc
    31261 ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa tgtattctcg atattcgccc
    31321 gctgaaagaa gaaaccggca tcagcgagct ggatattgcc aagcgcctga tcgactacgg
    31381 tttccacgcg ccgaccatgt cgttcccggt ggcgggtacg ctgatggttg aaccgactga
    31441 atctgaaagc aaagtggaac tggatcgctt tatcgatgcg atgctggcta tccgcgcaga
    31501 aattgaccag gtgaaagccg gtgtctggcc gctggaagat aacccgctgg tgaacgcgcc
    31561 gcacattcag agcgaactgg tcgccgagtg ggcgcatccg tacagtcgtg aagttgcagt
    31621 attcccggca ggtgtggcag acaaatactg gccgaccgtg aaacgtctgg atgatgttta
    31681 cggcgaccgt aacctgttct gctcctgcgt accgattagc gaataccagt aatttactga
    31741 ttcgactatc ttctaaaggc gcttcggcgc ctttttcgct tgttaataaa gtgcgctttg
    31801 tgtgtttcgg ttgaggcgta atataaaata ttgcgagctc aatatattaa aagagtgcca
    31861 ggcctgatga gcggagcaca tcaggcacat cggctaatta caaattctcg ccgttgctgg
    31921 caatcacctc tttgtaccag ttaaagctct tcttacgtga acgcgacata tcgccagtac
    31981 cgtcgtcatg tttattcaca tagataaagc cgtagcgttt gctgtactgc ccggtagtga
    32041 acgacacgca gtcgatgcag ccccacggtg tgtagcccat cagatccacg ccatcgtaag
    32101 tcaccgcttt tttcatctct tcaatatggg cgcgcaggta gtcaatgcgg taatcgtcat
    32161 tgatgctgcc atcatcttcc actttgtcgt aagcgccaaa accgttttcg acaataaaca
    32221 gcggcttctg gtaacgctca tacagctcgc aaagcgcgta acgcagacca accggatcaa
    32281 tctgccagcc ccagtcagac gctttaacat acgggttcgg tacgctgcct tcaaaaccag
    32341 agatcgcatc gccggtgccg ccttcggcct tcaccgcgtt ggtcatgtaa tagctgaagc
    32401 cgagataatc gcaggtgcct tcgcgcagta catccagatc gccgtcttcc attttgatgt
    32461 taaatccgcg gcgttcccac tcgttcaaca cataggacgg gtaatagcca cgcaactgca
    32521 catcggtaaa gacgtaacgt tcgcgcatcg acttctgggc aaacatcaca tcgtccgggt
    32581 tacaggagta gggatagagc ggcaccatcg ccagcataca accgactttc atctccgggt
    32641 tgatgcgatg cgcggctttc accgccaggg cgctggcgac aaactggtga tgcagcacct
    32701 gatacatggt ctcttccggg ttttcatgtt cggtatacac cacgccggag cagcagtaac
    32761 cgaacagcgg tgcacgccag ttacgctggt tgttaatttc gttgaaggtc atccagtatt
    32821 tgactttgtg cttatagcgt tcgaatacca cttccgcgaa acggacaaag aaatcaacca
    32881 ctttacggtt ggtccagcta ccatattgct gcaccagatg cagcggcatt tcaaagtggg
    32941 agagggtgat caccggttcg atgttgtatt tcagcagttc atcgatcata tcatcgtaga
    33001 acttcagccc ttcttcgttt ggctgagctt catcgccttt tggaaaaatg cgcgtccagg
    33061 cgatggaagt acgaaaacat ttgaagccca tttcggcaaa tagcttgatg tcctccttgt
    33121 agtgaccata aaaatcaacg gcttcatggt ttggatagta tttgccaggc acaacttctt
    33181 tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa atgctcggcc
    33241 ctttgccgcc tttgttccag ccgccttcga cctgatgagc ggcaactgcg ccgccccata
    33301 agaaatcttt cggtaaggtg agttttttca ctatcatagg ctccagatga gtcaattctt
    33361 acaggaaata ttaccatgca gccaaacgac atcacttttt ttcaacgttt ccaggatgac
    33421 attctggctg ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg
    33481 ggtgatgtgc ttcgtgtcgg acgttttgaa gatgacggtt atttttgcac gattgaagtc
    33541 accgcaacct caaccgtaac gctggatacg ctgacggaaa aacatgcaga gcaggaaaat
    33601 atgaccctga ctgaactgaa aaaggtcatt gccgacatct atcccgacca gacacagttt
    33661 tatgtgattg aatttaaatg tctttaaatt gaactcacgc ctgttagttg aaatttcgca
    33721 tttaatttca tgatttttcg gagattaatc tcaaatcaga atttatttta gctaacaggt
    33781 gttcactgga actattctca gttacgctgg agaggtatac atgtgcgtgt acgtttttcc
    33841 ggagtaagtt atggttcaga agcccctcat taagcaggga tattcgctgg cagaggaaat
    33901 tgccaacagc gtcagtcacg gcattgggct ggtgtttggt atcgttgggc tggtgttgct
    33961 actggttcag gcggtggatc tcaatgccag cgccacggcg ataaccagtt acagcctcta
    34021 tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca
    34081 acgggcaaaa atgtggctga agaaatttga ccactgcgct atttatctgt tgattgccgg
    34141 aacctacacg ccgtttttgc tggtggggct ggattctccg ttagcgcgcg ggttgatgat
    34201 tgttatctgg agcctggcat tgctgggtat tctgtttaaa ctgaccatcg cgcaccgatt
    34261 caaaatttta tctctggtga cctatctggc gatgggctgg ctgtcgctgg tggtaattta
    34321 tgaaatggca gttaagctcg cggcgggcag cgttacctta ctggcggtag gcggtgtggt
    34381 ttattcgctc ggggtgattt tctacgtctg caagcgcatt ccatacaatc atgccatctg
    34441 gcacggcttt gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt tgtatattgg
    34501 gcaggtgtaa ttggcgtgcc tttgtaggcc tgaaaagaca caccagtgtc gcatcaggcg
    34561 ataaatgcct gatgcgatac agagatttac tcttccagcg aatatggcag tggctcaata
    34621 cgcaatgtat tcgcatcgtc acgcacgcgg aagatgctgt ccggttccat atcgttattc
    34681 atgacaacct gcaccacaac ctgaccatct tccagtttga ccgcagccag caccgtaccg
    34741 gtacgacgcc agttctcgcc cattttcagc tctaagtctt caccagcttc cggcagtcgg
    34801 ctggcgctac ccgtcagcaa ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc
    34861 gccaccatct cttgtccggt atagcagcct ttcttaaagc tgatgccgcc cagcgcctgg
    34921 aggttggtcg cctgggggat aaactgcccg ctgttggcgg catcaatcac cggaaaaccc
    34981 gcttcaatgt ttaatgccag ccactgttgg ctattgttca gttccgcttc accgcgcagt
    35041 ttatcggtca gcatattggc agtagcttca tcggttacga tcaggaaacg ttctgccggg
    35101 tgttcaaacc atagcaacgt ggtcgcgcct tctttgacta cctgtttttc tctcgaaggt
    35161 agttcactaa agagatttgc caacgcggtg cgcgcctgaa aaccggcaac accaagcagc
    35221 acacgctcgt cgtctggcgc gatggtcact ttagagaaca ccgcatattt tttcagttca
    35281 gtcagctgcg gttcacgcac gctgcgccgt tcaatccatg caaagccatc gccgtcgcgg
    35341 aacagacgta aattgctcca cattttacct ttggcgtcgc aatgggcggc gagcaggtgc
    35401 tgatcttcag tcatctggct gacatctgct gtcacctggc cttgcatata tttttcgctg
    35461 tccgcgccag taatagtggc aagcgcccag tcatcaagcg tcatcagcgt cagtggcaaa
    35521 cgggcagaag ccgtaggctg acggggagga aaaggtgtaa aagccataat agagtcctga
    35581 gttgcttaac ggcgtattaa tcactaatgg taaaagagct atgaggcaat gcaagtgctt
    35641 tcactgcgcg atctgtgcct tctgcgaggc gacttccaaa aaaatgaagc ccttgaattg
    35701 ttgtgaatta atttcgcgat cgcgattgtg tttactgata ttcccgtaaa agaagtcgaa
    35761 aaacacgtta caatagccgg gtactggatt ttcgtgagac aggaagaaga tggacattaa
    35821 caacaaagcc cgcattcatt gggcatgccg ccgtggtatg cgcgaactcg atatttcaat
    35881 catgccgttt ttcgaacatg agtacgacag cttaagcgat gacgaaaaac gcacctttat
    35941 tcgtctgctg gaatgtgacg atccggacct gtttaactgg ctgatgaatc acggtaaacc
    36001 agccgatgca gaactggaaa tgatggtccg actcatccag acacggaacc gggaacgtgg
    36061 tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca gtggctttcc ttgctgattc
    36121 atgggctggt tgccgctgtt attttactca ttccctggcc gctcagttac accccgttat
    36181 ggatggtgtt actttcgctg gtggtgtttg attgcgttcg cagccagcgg cgcattaatg
    36241 ctcgccaggg ggaaattcgc ttgttgatgg acgggcgttt gcgttggcaa gggcaggagt
    36301 ggagcatcgt caaagcgccg tggatgatta agagcggcat gatgctgcgt ttacgttctg
    36361 acagcggaaa acggcaacat ttatggctgg cagctgacag catggacgaa gccgaatggc
    36421 gggatttacg gcggatattg ttgcaacaag agacgcaaag ataaagatga cggcaaagca
    36481 tatgtgctaa gccgtcagtg acatcaggcg taatgctctg ccatttcgtt gaggatttgc
    36541 tcgcaccagc tttgaatacg ctcgtcgcta aggtcatact ggttagtttc atccagcgcc
    36601 agacccacga acagttgccc gtcagcaatc accggtttcg gactggtaaa ttcatatcct
    36661 tccgttggcc agtagccgac gaacttcacg cctttggtcg agagtttgtc atgcagcata
    36721 ccgagcgcat cgaggaacca ctcgccgtat cccagttgat cgccaagccc atacagcgca
    36781 acaattttac cttcgaggtt caggtcgtcg agctgatccc agacggcttc ccagtcttcc
    36841 tggatttcgc caaaatccca ggtcgggata cccagaatga gcacatcgta ctgctccatt
    36901 aatttcgggg agtcgtcctt gaggttatgt aaggtcacca gttctgggcc gataatatcg
    36961 cggatttttt ctgccgccat ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata
    37021 ttcataacgt aagcttctcg attctgctgt ggcttttgcg gctagtgtat cagaatcgct
    37081 ttagtcacgg cacaataagg cataatggat caaatgagtg accataaggg gcgcaagtga
    37141 aacaggatct ggcacgcatc gagcagtttc ttgatgctct gtggctggaa aaaaatctgg
    37201 ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat gatggtggag tggttgcatc
    37261 accgcgggtt gacgctggcg acggcgcaaa gtgacgattt gcaggcatta ctggcagaac
    37321 ggctggaggg cgggtataaa gccaccagct cagcgcgttt gctgagtgca gtgcgccgat
    37381 tgttccagta tctttatcgc gaaaagtttc gtgaagatga tcccagtgcg catctcgctt
    37441 caccgaaatt gccccagcgt ttgccaaaag atttaagcga agcgcaggtc gaacgtttat
    37501 tacaggcacc attaattgat cagccactgg agctacgcga taaagccatg cttgaagtgt
    37561 tgtatgctac cggactgcgt gtctctgaac tggtcgggct gacaatgagt gatatcagcc
    37621 tgcgtcaggg cgtggtgcgg gtcattggta aaggcaacaa agagcgcctg gtgccgttag
    37681 gtgaagaggc ggtttactgg ctggaaacct atctggaaca tgggcgtccg tggctgttga
    37741 atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc gcagcagatg acgcgacaga
    37801 ccttctggca ccgcattaaa cattatgccg tgctggcggg tatcgacagc gaaaagctgt
    37861 caccgcatgt gttgcgtcac gcttttgcca ctcacttatt aaatcatggt gcggatttac
    37921 gcgtggtgca gatgctactg ggccacagcg atctctccac cacgcaaatt tatacgcatg
    37981 tcgctaccga gcgtctgcga caacttcatc aacagcatca cccgcgggcg tgatgtctga
    38041 aaagaacagg aaaatttatg aagaaaggtt ttatgttatt taccttgttg gcagcgtttt
    38101 caggctttgt tcaggctgat gacgcggcaa ttcaacaaac gttagccaaa atgggcatca
    38161 aaagcagcga tattcagccc gcgcccgtag ctggcatgaa gacagttctg actaacagcg
    38221 gtgtgttgta catcaccgat gatggtaaac atatcattca ggggccaatg tatgacgtta
    38281 gtggcacggc tccggtcaat gtcaccaata agatgctgtt aaagcagttg aatgcgcttg
    38341 aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg
    38401 atattacctg tggttactgc cacaaactgc atgagcaaat ggcagactat aacgcgctgg
    38461 ggatcaccgt gcgttatctt gctttcccgc gccaggggct ggacagcgat gcagagaaag
    38521 aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc gtttgatgat gtgatggcag
    38581 gtaaaagcgt cgcaccagcc agctgcgacg tagatattgc cgaccattac gcacttggcg
    38641 tccagcttgg cgttagcggt actccggcag ttgtgctgag caatggcaca cttgttccgg
    38701 gttaccagcc gccgaaagac atgaaagaat tcctcgacga acaccaaaaa atgaccagcg
    38761 gtaaataatt cgcgtgaaac aacagataca acttcgtcgc cgtgaagtcg atgaaacggc
    38821 cgacttgccc gctgaattgc ctcccttgct gcgccgttta tatgccagcc ggggtgtgcg
    38881 tagcgcgcag gaactggaac gcagtgttaa aggcatgctg ccctggcagc aactgagcgg
    38941 cgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc gaaggaacgc ggattattgt
    39001 ggtcggtgat ttcgacgccg acggtgccac cagcacggct ctaagcgtgc tggcgatgcg
    39061 ctcgcttggt tgcagcaata tcgactacct ggttccaaac cgtttcgaag acggttacgg
    39121 cttaagcccg gaagtagtcg atcaggctca tgcccgtggc gcgcagttaa ttgtcacggt
    39181 ggataacggt atttcctccc atgcgggggt tgaacacgct cgttcgttgg gcattccggt
    39241 tattgttacc gatcaccatt tgccaggcga cacattaccc gcagcggaag cgatcattaa
    39301 ccctaacttg cgcgactgta atttcccgtc gaaatcactg gcgggcgttg gcgtggcgtt
    39361 ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag ggctggtttg atgagcgcgg
    39421 catcgcaatt cctaatctgg cagaactgct ggatctggta gcgttgggga cagtggcgga
    39481 cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg caggggatga gtcgcatccg
    39541 tgccggaaag tgccgtccgg ggattaaagc gttgctggaa gtggccaatc gcgatccaca
    39601 aaaactcgcc gccagcgatt taggttttgc gctggggcca cgtctcaatg ctgccgggcg
    39661 actggacgat atgtccgttg gcgtggcgct gttgctgtgc gacaacatcg gcgaagcgcg
    39721 cgtgctggca aatgaactcg atgcgctaaa ccagacgcga aaagagatcg aacagggaat
    39781 gcaagttgaa gccctgacct tgtgcgagaa actggagcgc agccgcgaca cgctacccgg
    39841 cgggctggca atgtatcacc ccgaatggca tcagggcgtt gtcggtattc tggcttcgcg
    39901 catcaaagag cgttttcatc gtccggttat cgcctttgcg ccagctggtg atggtacgct
    39961 gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt gatgcgctgg agcggctaga
    40021 cacgctttac cccggcatga tgctcaagtt tggcggtcat gcgatggcgg cgggtttgtc
    40081 gctggaagag gagaaattcg aactctttca acaacggttt ggcgaactgg ttactgagtg
    40141 gctggaccct tcgctattgc aaggcgaagt ggtgtcggac ggtccgttaa gtccggccga
    40201 aatgaccatg gaagtggcgc agctgttgcg cgatgctggc ccgtgggggc agatgttccc
    40261 ggagccgctg tttgatggtc atttccgtct gctgcaacag cggctggtgg gcgaacgtca
    40321 tttgaaagtc atggtcgaac cggtcggcgg cggtccactg ctggacggga ttgcttttaa
    40381 tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa gtgcaactgg cttacaagct
    40441 cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt atcatcgaca atatctggcc
    40501 aatttagcgt catcttctct ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc
    40561 accgcatttt gactagctca ataaaagaaa tcagaccatg tttgaaatta atccggtaaa
    40621 taatcgcatt caggacctca cggaacgctc cgacgttctt agggggtatc tttgactacg
    40681 acgccaagaa agagcgtctg gaagaagtaa acgccgagct ggaacagccg gatgtctgga
    40741 acgaacccga acgcgcacag gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg
    40801 acaccctcga ccaaatgaaa caggggctgg aagatgtttc tggtctgctg gaactggctg
    40861 tagaagctga cgacgaagaa acctttaacg aagccgttgc tgaactcgac gccctggaag
    40921 aaaaactggc gcagcttgag ttccgccgta tgttctctgg cgaatatgac agcgccgact
    40981 gctacctcga tattcaggcg gggtctggcg gtacggaagc acaggactgg gcgagcatgc
    41041 ttgagcgtat gtatctgcgc tgggctgaat cgcgtggttt caaaactgaa atcatcgaag
    41101 agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat caaaatctcc ggcgattacg
    41161 cttatggctg gctgcgtaca gaaaccggcg ttcaccgcct ggtgcgtaag agcccgtttg
    41221 actccggcgg tcgtcgccac acgtcgttca gctccgcgtt tgtttacccg gaagttgatg
    41281 atgatatcga tatcgaaatc aatccagcgg atctgcgcat cgacgtttat cgcgcatccg
    41341 gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt gcgtattact cacatcccaa
    41401 ccgggatcgt gacccaatgc cagaacgacc gttcccagca caagaacaaa gaccaggcca
    41461 tgaagcagat gaaagcgaag ctttatgaac tggagatgca gaagaaaaat gccgagaaac
    41521 aggcgatgga agataacaaa tccgacatcg gctggggcag ccagattcgt tcttatgtcc
    41581 ttgatgactc ccgcattaaa gatctgcgta ccggggtaga aactcgcaac acgcaggccg
    41641 tgctggacgg cagcctggat caatttatcg aagcaagttt gaaagcaggg ttatgaggaa
    41701 ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt agtcgatctt aacaatgaac
    41761 tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca ggggattgcc ttcccgaacg
    41821 atttccgtcg cgatcatacc tctgaccaat tgcacgcaga attcgacggt aaagagaacg
    41881 aagaactgga agcgctgaac atcgaagtcg ccgttgctgg ccgcatgatg acccgtcgta
    41941 ttatgggtaa agcgtctttc gttaccctgc aggacgttgg cggtcgcatt cagttgtacg
    42001 ttgcccgtga cgatctgccg gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg
    42061 gcgatatcct cggcgcgaaa ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc
    42121 actgtaccga gctgcgtctg ctgaccaaag cactgcgtcc gctgccggat aaattccacg
    42181 gcttgcagga tcaggaagcg cgctatcgtc agcgttattt ggatctcatc tctaacgatg
    42241 aatcccgcaa cacctttaaa gtgcgctcgc agatcctctc tggtattcgc cagttcatgg
    42301 tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca ggtgatccct ggcggtgccg
    42361 ctgcgcgtcc gtttatcact caccataacg cgctggatct cgacatgtac ctgcgtatcg
    42421 cgccggaact gtacctcaag cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca
    42481 accgtaactt ccgtaacgaa ggtatttccg tacgtcataa cccagagttc accatgatgg
    42541 aactctatat ggcttacgca gattacaaag atctgatcga gctgaccgaa tcgctgttcc
    42601 gtactctggc acaggatatt ctcggtaaga cggaagtgac ctacggcgac gtaacgctgg
    42661 acttcggtaa accgttcgaa aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg
    42721 aaaccgacat ggcggatctg gacaacttcg actctgcgaa agcgattgct gaatctatcg
    42781 gcatccacgt tgaaaagagc tggggtctgg gccgtatcgt taccgagatc ttcgaagaag
    42841 tggcagaagc tcatctgatc cagccgacct tcattactga atatccggca gaagtttctc
    42901 ctctggcgcg tcgtaacgac gttaacccgg aaatcacaga ccgctttgag ttcttcattg
    42961 gtgggcgtga aatcggtaac ggctttagcg aactgaatga cgcggaagat caggcgcagc
    43021 gcttcctgga tcaggttgcc gcgaaagatg caggtgacga cgaagcgatg ttctacgacg
    43081 aagattatgt caccgcactg gaacatggct taccgccgac agccggtctg ggaattggta
    43141 tcgaccgtat ggtgatgctg ttcaccaaca gccataccat ccgcgacgtt attctgttcc
    43201 cggcgatgcg tccggtaaaa taagcattac gttatgctca caaccccggc aaccgtcggg
    43261 gttttttatt taagttgggt aaatgcagat aatcgttttc tggcttcgcg atttgtcgcc
    43321 tgcatcacca tccacggact gaacgcccac ggcgtagcat caataccgcg taaaaccgct
    43381 gctaaatcac accattgata atccatcact tcatcatcgt tgatctgtaa cgcactggtc
    43441 gtgcgtgcgg caaataccgg acacacttca ttttccacaa tgccattcgg atcggtggcg
    43501 cggtagcgaa agtcaggata gatagattca ggaggcgtaa tttctacgcc aagctcataa
    43561 cggcaacggc ggatcaccgc ctcttcgttg ctttctccca gttgtgggtg cccacaaacc
    43621 gagttagtcc acacgccagg ccatgctttt ttgctaaggg cgcggcgggt aactaataat
    43681 tgccctttgg cattaaacag ccaactggaa aacgcgagat gtaagagggt gtctgccgtg
    43741 tgtgcggcat acttttccag cgtacccgtg ggaactccct gtgcattcaa taaaatgacg
    43801 tgttccgttt gcataatttc tcacatgtaa ttctgatcac tcatcattat agataataga
    43861 agagtgaaat gattaaccaa gtgagcgttt atcgacaacc gcccgttttg agcggatgtc
    43921 gacaggtaaa aaccatttaa aaaagcagca tcatatattt gaagcgactt tcaatggcca
    43981 ttgcaacaca atcagaaatg acaggaatag agagagtaaa aagcaacagc agactgaata
    44041 tagcgcgcat taaaagattc atattattct ccatgctcat ttttcttcag cttgcgcatc
    44101 agaagccaca tacgtactgt cctgactttt aaatctttat cagagacgga ttccttcacc
    44161 aggtcagcat gatgctggta gccattaaag aagacgttca gcacaacggc gctaagggtt
    44221 gcgagcataa tgccgctatg cagcaacggt tgcagtacgg ccggtaactt agaaaagaaa
    44281 tcgtgagaga gcgttggcgt catgccaaca ccgagactga tcgccacaat atagaggttg
    44341 taacggttag tggtgtagtt acagcgcgac agaattcgaa tccctgtcgc cagtaccatg
    44401 ccgaacatca ccagccccgc gccgcccagc acaaattgcg gaatggaggc gaccagcacc
    44461 gccattttgg gcaccatgcc aaacaggatt aatataattc ccgaagaaat acacacccag
    44521 cggctgtgga cgcgcgtcac gctgaccagc ccgacgttct gcgaaaatga cgtatgtggg
    44581 aagctgttaa acgtgcctcc aatcattgtc ccgaccccat ctacgcgtaa cccacgaata
    44641 atgtcctggg aagagagttt gcgaccgact atttcaccca gtgccaggaa catccccatt
    44701 gactcaataa atacgatgat gagtactgcg gtcatggtca aaatggaaac gggatcgaaa
    44761 atcggcatac caaatgacat cggcgtgaca atcgcaaacc atgaagcatc atgtagcccg
    44821 gataaattga cttcattcat catccacgaa agtaaaaagc caaatacaat ccccagtaat
    44881 acggcgacgt tggacataaa tcctttcgca tagcgagtaa tgagcaagat aaaaattaag
    44941 acggcaaagg agatacctaa ataaacggga ttaccatatt gcggattccc tttacctccg
    45001 gcggcccagt caatacccac ctgaatgatg ctaagcccga tagaagtaat caccacaccg
    45061 gtaaccagtg gcgggaataa aggcatcaag cgaccgataa gtggcgctaa taatgtggtg
    45121 ataaaacccg cggcgatagt ggcaccgaat atccccagca ggccgatatc cgggttcata
    45181 ccaatggcta tcattggtgt tacagcggcg aaggtcaccg acataatcac cggcaggcgg
    45241 atccccataa agcggccgat accgatacat tgcaataatg tgacgatccc gcagcaaaag
    45301 agatccgagc taatgagcat cgcaatagct tcttttgata ggccgagtcg gtcaccaatc
    45361 ataagaggaa cagcgactgc acctgcgtac atgacaagga cgtgttgcag accgagtatg
    45421 atcagctttc ctggtgataa tatgcggtca acctcatcag ttgggcggtc ttgcccagaa
    45481 gatgagggaa gttgggaatc tatggcgctc atcagtgttt ctcctttcgc ccgccagagt
    45541 aaaaagcaac cgtttatgtt catgaaactg tctaaacaca tgctcgtgga gtgccagttt
    45601 ttgcgaggaa ggggagttag agacaggaat tgcggaggca atcacataat cgtggttaaa
    45661 ccacgaaaat gagaagagaa cctgacaa
//
LOCUS       DAPQZD010000017        44815 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPQZD010000017 DAPQZD010000000
VERSION     DAPQZD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.017"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(328..870)
                     /locus_tag="RO454_000971"
     CDS             complement(328..870)
                     /locus_tag="RO454_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0932841.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(893..1918)
                     /gene="nagZ"
                     /locus_tag="RO454_000972"
     CDS             complement(893..1918)
                     /gene="nagZ"
                     /locus_tag="RO454_000972"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDY0932842.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1929..2753)
                     /gene="thiK"
                     /locus_tag="RO454_000973"
     CDS             complement(1929..2753)
                     /gene="thiK"
                     /locus_tag="RO454_000973"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDY0932843.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2734..3375)
                     /gene="lpoB"
                     /locus_tag="RO454_000974"
     CDS             complement(2734..3375)
                     /gene="lpoB"
                     /locus_tag="RO454_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDY0932844.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3389..3766)
                     /locus_tag="RO454_000975"
     CDS             complement(3389..3766)
                     /locus_tag="RO454_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDY0932845.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3769..4128)
                     /gene="hinT"
                     /locus_tag="RO454_000976"
     CDS             complement(3769..4128)
                     /gene="hinT"
                     /locus_tag="RO454_000976"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDY0932846.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4462..6651
                     /gene="fhuE"
                     /locus_tag="RO454_000977"
     CDS             4462..6651
                     /gene="fhuE"
                     /locus_tag="RO454_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDY0932847.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGTYQF"
     gene            complement(6711..8144)
                     /gene="ptsG"
                     /locus_tag="RO454_000978"
     CDS             complement(6711..8144)
                     /gene="ptsG"
                     /locus_tag="RO454_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDY0932848.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8439..9236)
                     /locus_tag="RO454_000979"
     CDS             complement(8439..9236)
                     /locus_tag="RO454_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDY0932849.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9247..10251)
                     /gene="holB"
                     /locus_tag="RO454_000980"
     CDS             complement(9247..10251)
                     /gene="holB"
                     /locus_tag="RO454_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDY0932850.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10248..10889)
                     /locus_tag="RO454_000981"
     CDS             complement(10248..10889)
                     /locus_tag="RO454_000981"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDY0932851.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(10879..11901)
                     /gene="yceG"
                     /locus_tag="RO454_000982"
     CDS             complement(10879..11901)
                     /gene="yceG"
                     /locus_tag="RO454_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDY0932852.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11904..12713)
                     /gene="pabC"
                     /locus_tag="RO454_000983"
     CDS             complement(11904..12713)
                     /gene="pabC"
                     /locus_tag="RO454_000983"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDY0932853.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12833..14074)
                     /gene="fabF"
                     /locus_tag="RO454_000984"
     CDS             complement(12833..14074)
                     /gene="fabF"
                     /locus_tag="RO454_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDY0932854.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14162..14398)
                     /gene="acpP"
                     /locus_tag="RO454_000985"
     CDS             complement(14162..14398)
                     /gene="acpP"
                     /locus_tag="RO454_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDY0932855.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14609..15343)
                     /gene="fabG"
                     /locus_tag="RO454_000986"
     CDS             complement(14609..15343)
                     /gene="fabG"
                     /locus_tag="RO454_000986"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDY0932856.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15356..16285)
                     /gene="fabD"
                     /locus_tag="RO454_000987"
     CDS             complement(15356..16285)
                     /gene="fabD"
                     /locus_tag="RO454_000987"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDY0932857.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16301..17254)
                     /gene="fabH"
                     /locus_tag="RO454_000988"
     CDS             complement(16301..17254)
                     /gene="fabH"
                     /locus_tag="RO454_000988"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDY0932858.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17322..18392)
                     /gene="plsX"
                     /locus_tag="RO454_000989"
     CDS             complement(17322..18392)
                     /gene="plsX"
                     /locus_tag="RO454_000989"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDY0932859.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18473..18646)
                     /gene="rpmF"
                     /locus_tag="RO454_000990"
     CDS             complement(18473..18646)
                     /gene="rpmF"
                     /locus_tag="RO454_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDY0932860.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18698..19219)
                     /gene="yceD"
                     /locus_tag="RO454_000991"
     CDS             complement(18698..19219)
                     /gene="yceD"
                     /locus_tag="RO454_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDY0932861.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19418..20002
                     /gene="yceF"
                     /locus_tag="RO454_000992"
     CDS             19418..20002
                     /gene="yceF"
                     /locus_tag="RO454_000992"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDY0932862.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20114..21073)
                     /gene="rluC"
                     /locus_tag="RO454_000993"
     CDS             complement(20114..21073)
                     /gene="rluC"
                     /locus_tag="RO454_000993"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDY0932863.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21646..24831
                     /gene="rne"
                     /locus_tag="RO454_000994"
     CDS             21646..24831
                     /gene="rne"
                     /locus_tag="RO454_000994"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDY0932864.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25026..25979)
                     /gene="flgL"
                     /locus_tag="RO454_000995"
     CDS             complement(25026..25979)
                     /gene="flgL"
                     /locus_tag="RO454_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDY0932865.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25991..27634)
                     /gene="flgK"
                     /locus_tag="RO454_000996"
     CDS             complement(25991..27634)
                     /gene="flgK"
                     /locus_tag="RO454_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDY0932866.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27700..28641)
                     /gene="flgJ"
                     /locus_tag="RO454_000997"
     CDS             complement(27700..28641)
                     /gene="flgJ"
                     /locus_tag="RO454_000997"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDY0932867.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28641..29738)
                     /gene="flgI"
                     /locus_tag="RO454_000998"
     CDS             complement(28641..29738)
                     /gene="flgI"
                     /locus_tag="RO454_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDY0932868.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29750..30448)
                     /gene="flgH"
                     /locus_tag="RO454_000999"
     CDS             complement(29750..30448)
                     /gene="flgH"
                     /locus_tag="RO454_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDY0932869.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30502..31284)
                     /gene="flgG"
                     /locus_tag="RO454_001000"
     CDS             complement(30502..31284)
                     /gene="flgG"
                     /locus_tag="RO454_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDY0932870.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31456..32211)
                     /gene="flgF"
                     /locus_tag="RO454_001001"
     CDS             complement(31456..32211)
                     /gene="flgF"
                     /locus_tag="RO454_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDY0932871.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32231..33436)
                     /gene="flgE"
                     /locus_tag="RO454_001002"
     CDS             complement(32231..33436)
                     /gene="flgE"
                     /locus_tag="RO454_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDY0932872.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWDVYTLDSSDP
                     TGTANPATTLVFNANGVLTSDPTKDITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33461..34156)
                     /gene="flgD"
                     /locus_tag="RO454_001003"
     CDS             complement(33461..34156)
                     /gene="flgD"
                     /locus_tag="RO454_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDY0932873.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34168..34572)
                     /gene="flgC"
                     /locus_tag="RO454_001004"
     CDS             complement(34168..34572)
                     /gene="flgC"
                     /locus_tag="RO454_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDY0932874.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34576..34992)
                     /gene="flgB"
                     /locus_tag="RO454_001005"
     CDS             complement(34576..34992)
                     /gene="flgB"
                     /locus_tag="RO454_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDY0932875.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35147..35806
                     /gene="flgA"
                     /locus_tag="RO454_001006"
     CDS             35147..35806
                     /gene="flgA"
                     /locus_tag="RO454_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDY0932876.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            35882..36175
                     /gene="flgM"
                     /locus_tag="RO454_001007"
     CDS             35882..36175
                     /gene="flgM"
                     /locus_tag="RO454_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDY0932877.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36180..36596
                     /gene="flgN"
                     /locus_tag="RO454_001008"
     CDS             36180..36596
                     /gene="flgN"
                     /locus_tag="RO454_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDY0932878.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(36636..38171)
                     /gene="murJ"
                     /locus_tag="RO454_001009"
     CDS             complement(36636..38171)
                     /gene="murJ"
                     /locus_tag="RO454_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDY0932879.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38281..39204)
                     /locus_tag="RO454_001010"
     CDS             complement(38281..39204)
                     /locus_tag="RO454_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDY0932880.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39206..39853)
                     /locus_tag="RO454_001011"
     CDS             complement(39206..39853)
                     /locus_tag="RO454_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDY0932881.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39864..40448)
                     /gene="rimJ"
                     /locus_tag="RO454_001012"
     CDS             complement(39864..40448)
                     /gene="rimJ"
                     /locus_tag="RO454_001012"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDY0932882.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40684..41892
                     /gene="mdtH"
                     /locus_tag="RO454_001013"
     CDS             40684..41892
                     /gene="mdtH"
                     /locus_tag="RO454_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDY0932883.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41956..42603
                     /gene="grxB"
                     /locus_tag="RO454_001014"
     CDS             41956..42603
                     /gene="grxB"
                     /locus_tag="RO454_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDY0932884.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42736..43296
                     /locus_tag="RO454_001015"
     CDS             42736..43296
                     /locus_tag="RO454_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0932885.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43402..44445
                     /gene="pyrC"
                     /locus_tag="RO454_001016"
     CDS             43402..44445
                     /gene="pyrC"
                     /locus_tag="RO454_001016"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDY0932886.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVK"
     gene            44522..44767
                     /gene="dinI"
                     /locus_tag="RO454_001017"
     CDS             44522..44767
                     /gene="dinI"
                     /locus_tag="RO454_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDY0932887.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10834 a        12103 c        11154 g        10724 t
ORIGIN      
        1 ttatagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca ttggtcatac
       61 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggtgagat
      121 caaaaaacag agtttgttgt tcattatttg agctgatttg tgagaatgat atagagtgag
      181 gtgtgtatgg agggcgttcg gtaggcctga taagaggcgc atcaggcatt gatgctcgac
      241 tgtcggatgc ggcggaacgc cttatccaac ccacagaatc aggagcgcat caggtaccgg
      301 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
      361 taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
      421 ataatgatgc aactcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
      481 aatcaccagg caacgatcgc gattcttctc acggaagttg gtcacacact tagtggcaat
      541 atcggcatac tcttccgggc gatcaatctt cccttccatg ttctcataag ggaacaaatt
      601 ggggttgaag atcacctggc ggatatcaca gagaaaacca atccgctcag cccaataacc
      661 gcccagacca acaccacaaa tgagcgggcg ctcgtcgacg ttcagttgca acattttgtc
      721 cacttctttg agcaaatgct gcatatcatg ttttggatgc cgcgtactgt agcttattag
      781 ccgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
      841 agagtcaaaa ccgtgcaaat agataatcat cgcttcctca cataagccag ggttaatgac
      901 ctgccttctc ttcctgccag cgttcatgta actgattcag acgggcgctg atcgctttcc
      961 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
     1021 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
     1081 tgcagaccag gatcatatcg caacctgcat ccagtgatgc ctgtccacgc tcggcataac
     1141 tgcccataat cgctgcgcct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
     1201 ccaattcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc ggacgcggat
     1261 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
     1321 aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgcggacgt gggtcgcacg
     1381 gtgtttcttt gtgtgagtct gccgttactg caccgtgacc tgggaagtgt ttcccggtag
     1441 ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt
     1501 gtggatcggc atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acgtccagca
     1561 ctggcgcaaa gctgatatca atatccatag caatcatttc gctggccatc aaccaacctg
     1621 cctcttgcgc cagttttccg ccttcttcca ttcccaacag cgcagcgaat gattgtgccg
     1681 ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg
     1741 ccaccaccag atgatttcgc gatgctgcgc ggatctggcg caccagttca cgtaactggg
     1801 caggatcatg atagtttcgc gtaaagagaa tcagccctcc caccagcgga tgcgccagta
     1861 tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac
     1921 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
     1981 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
     2041 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
     2101 gcatagtcat tgaccaattg ccgatgctgg tcagtatttt ccacccacac cgccgccagt
     2161 tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg
     2221 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaagccgt
     2281 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
     2341 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
     2401 cgcggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
     2461 gtttttacct cgccgggcag atagtcaacc accatccagt cacggagata taaatgcggc
     2521 ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
     2581 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
     2641 agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
     2701 aagcgcgaca gcaattcgtc gcgcgtgagg ggattattgc tgcgaaacgg cacctttacc
     2761 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
     2821 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccaacattgc gggcaatgcc
     2881 tatggcttta ctacgggtgc ctaaactgtc ctgcggcgac aaacctaatt gttgcttagc
     2941 catcgacagc tgctgggcgg aaaccagggt aaatttaccg ttattagcca gcgcatttcg
     3001 cagcgtttcg gtcgcttctg cggcattcaa cgaaccgtta gtacggttgt taacgctatc
     3061 aaccagcagg acgctacccg cagtcacgcc atcagcccca agcatcttac tgaccatcgg
     3121 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg cagtttgatc
     3181 ttcgtgctca attgggcctg gctgctgcgg tatcgtcggc accgatggca ctgtggggac
     3241 aggctgttgt ggctcggttg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
     3301 ttcacgttgc cccacacacc cggcaagaaa catcgccagc gcggtaatca aggcgtagcg
     3361 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
     3421 cccccagaaa attggcgctg ccgtacagcg tgaccgccga atgtgcggga atggtaacgc
     3481 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     3541 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
     3601 tatcagatgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     3661 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     3721 gcactaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     3781 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     3841 ctcttgtccg ccatggcggt tggtgttcat gatcagacga tagccatctt cggcaatacc
     3901 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     3961 agctgacacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
     4021 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     4081 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     4141 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     4201 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaaccgtttt
     4261 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattt
     4321 tctgtttggt ttacacacca aatatgaatg cgtatatttc tcatttgcat ttacatacaa
     4381 aattattcgc acactaaata taaccgctca tcaccctgct ccggcatgag cctgacgcca
     4441 acataaacca agagatttca aatgctttcg acacaattta acagggataa tcaacatcaa
     4501 gccatcatca aaccgtcact ccttgccggt tgcatagcac tggcactatt accttccgcc
     4561 gcttttgctg caccagccac tgaagaaacg gtgattgttg agggttcagc cacagcccca
     4621 gatgatggcg aaaatgatta caacgtaacg tctacctctg cgggtacaaa aatgcagatg
     4681 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
     4741 cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg
     4801 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
     4861 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
     4921 ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggcc tcatgaccgg gacgggtaat
     4981 ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat
     5041 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
     5101 ctcaccgaag acggtaaaat ccgcgcacga attgtcggcg gctaccagaa taacgactca
     5161 tggctggacc gctacaacag tgaaaagacc ttcttctccg gcattgtcga tgctgattta
     5221 ggcgatctta cgatgctttc agccggttac gaatatcagc gtattgatgt taatagccca
     5281 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgcgca
     5341 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
     5401 ctgaagcagc ggtttgctga tacctggcaa gcgacgctga atgccaccca ctctgaagtc
     5461 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
     5521 gttgggccat acagtaatta tggccctggt tttgattatg tcggaggcac cggttggaac
     5581 agcggcaaac gtaaagttga tgcgctggat ctgttcgctg acggtagtta tgaattgttt
     5641 ggtcgtcagc acaatttaat gtttggtggc agttacagca aacaaaacaa tcgttacttc
     5701 agttcatggg ccaacatttt cccggatgaa attggcagtt tctacaactt taatggcaat
     5761 ttcccacaaa ccgacaggtc accacagagt ctggcgcagg acgataccac acatatgaaa
     5821 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
     5881 cgttatacca agtggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
     5941 ccttatgctg gactggtgtt tgacatcaat gataactggt cgacctacgc cagctatacc
     6001 tctatttttc agccgcaaaa tgatcgtgac agttcaggca aatatctggc accaatcacc
     6061 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
     6121 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
     6181 ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa aggtgtggaa
     6241 tttgaactca acggcgcaat taccgacaac tggcagctga cgtttggcgc aacgcgctat
     6301 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
     6361 atgttcacca gctatcggtt gcctgtcatg ccagagttga cggtcggcgg tggtgttaac
     6421 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
     6481 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
     6541 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga aggttctatt
     6601 gtctacggcg caccgcgtaa tttaagcatt accggcacgt atcaattctg aataacgcct
     6661 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacgga ttagtggtta
     6721 cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa aatcgcctga
     6781 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc
     6841 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
     6901 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtc
     6961 gcttttgcgt cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
     7021 agcacgcgga agatggtgta gtaaacaatg gcataaccga taccgacgat cgggaacagc
     7081 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
     7141 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
     7201 tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata
     7261 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt
     7321 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
     7381 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
     7441 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
     7501 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
     7561 aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagagcca
     7621 ataggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagtcag gcctgaaatg
     7681 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
     7741 aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccccccgag tacgccagta
     7801 tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
     7861 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
     7921 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
     7981 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
     8041 gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc
     8101 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
     8161 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagtgcc tcgccgtgta
     8221 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccgatttgt
     8281 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
     8341 tatgtcacaa tatttcaaac gtggttattc accgcccgat tttcggcggt gaactttact
     8401 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
     8461 gaagcatcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
     8521 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
     8581 ttctcttttc ctcgatgcgg tactggcgca aggtaaggtg agtcagtttc caccagtaac
     8641 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
     8701 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
     8761 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
     8821 agcgtatcgg cgcgggcgtc acgggtgtgg acgataaccg gcttgttcag ttcacgacca
     8881 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg cgtgtaataa
     8941 taatccagcc cggtttcacc cagcgctaca acgccctctt ctgccgccag acgacgtaaa
     9001 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
     9061 ttgtcgcgtt cgcctaccag atcccgcatg tgtaaataac ccggtaatgt tgtggcgact
     9121 gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
     9181 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg
     9241 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
     9301 cagtaaaaga tcggtgatga gaagctcgcg gttgatacct gtgacagaca ttaactgttc
     9361 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
     9421 cagttcgacg accagccccg gcacatcaac attggtcacg tgcgcagcgc cataatggcg
     9481 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatt
     9541 attaagggcc gctagcagcg aataccagtc gcccgattgc acgccatatg ccagcgcctg
     9601 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgccccagg
     9661 cgaaccggcg cttaagcgca atgcggcaag tagtgcatcc tgtgacattg tcacttcgcg
     9721 tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg
     9781 actacgtaat gttgccagta aacgttctgg ctcgcgggta gcaaggaaaa accacgtctc
     9841 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg ttagtaaggc
     9901 agcatcggtt acccagacaa ctttcgcacc acctaagcgt gcgtgctcat tcagcttttc
     9961 ggtgacctca cgtaccgcat caatgcccag cgcatttttc cctttttcgg gagccagggt
    10021 gtagtaatcg ggatgtgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    10081 tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    10141 atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata
    10201 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    10261 tccttcaccc aattggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    10321 gtggcatcaa tggtatgaat gcttttatct tgtgctgcca gttccagata gcgggcgcgg
    10381 gtgcgattaa agaaatcgaa tgactcttgc tcaatacgat ccagctcacc acgggcgcga
    10441 gcacgtttta agccgacctc tggggtgaca tcaagataaa gcgttaagtc agggcgaaaa
    10501 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctcca
    10561 ccctgatagg cttgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    10621 agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcaaaact
    10681 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    10741 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    10801 agttgctcaa gcgtctcaac caccacgtta cgcgcggtag ttttgcctgc gccttccagc
    10861 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    10921 cacagacttg ttatgactgg caagattggt attaaacgtg tgaccacctt taccatcggc
    10981 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    11041 cggcgtcgct atcgcacctg gcggcagacc ggtaatggta taggtgttat acgctgtcgg
    11101 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    11161 ggtcgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ctgaggcaac
    11221 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccaa
    11281 ctggttttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    11341 tttcaccatt ttcttgtgcg ctcgtttgag taacgcgaca tcggtggtat tggcggtata
    11401 catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    11461 ctgcgctacg gtggcgtact tatcgtcgct cagcgtgtgc ttgatatacg gagcctcacg
    11521 caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc
    11581 ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    11641 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attggaaaac
    11701 ccgtgggcga ttgatgatct tatcggcata aagctgttca ccgagcgcca gacgtccggt
    11761 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    11821 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    11881 tatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    11941 tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt
    12001 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga ggcttgcact
    12061 tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg
    12121 ttaacacctg cctgatccag tcgcggcgta taaactacgt tgcccttccg ccagaacaaa
    12181 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    12241 tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag atgtttaata
    12301 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg
    12361 ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg cgttgccggt
    12421 ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagt
    12481 acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag
    12541 tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac
    12601 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    12661 gcccgatcgc ttactgccag cgattcctgc ttatgaccgt taattaagaa cataccggct
    12721 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    12781 ctaagaaaaa aggcccgcaa gcggaccttt tataagggtg gaaaatgaca atttagatct
    12841 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggaattacac agagtgtatt
    12901 ccattccgct aacctgacgc gcttcgtgcg gtacgaaatc caaatcgcaa ccttcatccg
    12961 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgcaagg atggagtaga
    13021 tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta
    13081 ccaacacgcg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    13141 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    13201 ctgcatcacg cagagcattt gccatcgcca gagctgcgcc tgcgccattt tctggcggtg
    13261 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    13321 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    13381 gtacgaaacc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    13441 gggtagataa tgcgcgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    13501 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatggc
    13561 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    13621 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    13681 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    13741 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    13801 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    13861 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    13921 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    13981 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    14041 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat
    14101 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    14161 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    14221 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    14281 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    14341 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    14401 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    14461 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    14521 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    14581 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    14641 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    14701 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    14761 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    14821 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    14881 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    14941 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    15001 aaacagatga aaggttggtt tcgataatat cgttccactc ttcatctttc attcgcatta
    15061 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    15121 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    15181 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    15241 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    15301 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    15361 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    15421 caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc gacttcatag agatgttcta
    15481 cgccttgcgc tgccatgtat tcaacagact tcgtccactg aaccgggtta tacaactgac
    15541 gtaccagtgc gtcacgaatg gcatcaccat tggtttcgca tttcacatca acgttattca
    15601 caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    15661 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtagcggc agagcgcgtt
    15721 ttgcgcccgc agctttacaa gcagcacccg cacgctcaac cgcttcttta tgaccggcaa
    15781 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    15841 cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcacccg
    15901 ttccttccgg tacggcttct tgcatgaact tgccgcgcat ctcaaccaga cgcaccgcat
    15961 cagcgaaatc aatcacacca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    16021 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    16081 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    16141 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    16201 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    16261 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg
    16321 agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    16381 gcccgtcgcg tacagcttca tccagagcgc acgggacaga ggccgcagag gtattaccgt
    16441 ggcgatccag cgtcaccacg acattgtcca tcgacatgcc gagttttttc gctgttgcac
    16501 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    16561 tattcgccgt cagcgtctca tcaacgatgt gcgccagttc tgttaccgca accttgaaga
    16621 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacacgg tcagcattag
    16681 gcagcgtcag caactcacca tagctaccgt cggcatgcag atgggtggag atgattcccg
    16741 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    16801 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    16861 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc
    16921 aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc cagcatgctt tgaatctgac
    16981 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    17041 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    17101 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    17161 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    17221 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    17281 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    17341 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    17401 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    17461 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    17521 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    17581 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    17641 gaaaggaaca tcctgacaac accttccatc gtctttaatg tgacatttcc tgtaaagccg
    17701 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    17761 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc
    17821 tttacttctt cttcaccaat attgagcaac gccacgcgag gattaggaat ttccaccacc
    17881 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtact gtcacaatcg
    17941 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgcgg taataccgtc
    18001 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatt
    18061 agcgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    18121 agggccacgc gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    18181 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    18241 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    18301 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    18361 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    18421 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt aattacttag
    18481 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    18541 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    18601 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    18661 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    18721 atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag accatgtccg
    18781 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    18841 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    18901 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    18961 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    19021 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    19081 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcaggag
    19141 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    19201 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    19261 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    19321 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    19381 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctgaactta ttttagcctc
    19441 cacctcgccc tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    19501 acccgaggtc gacgaaactc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    19561 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    19621 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    19681 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    19741 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    19801 tcacctgagc gaggcggaga ttgataatta cgtgcgtaaa gagcatcccc tgcactgcgc
    19861 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag aggggcgtga
    19921 tcctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    19981 aaaaaacccg ctgatgggat agttttttgc gccggatgcg gttacgtcgc atccggcaat
    20041 cggtgctggc ggcctgatgc ggcataaacg ccttatcagg ccaaccgccc attatcatgc
    20101 ttatatgctc gtattagcgc gcgttacgca gtttttgcaa acaacgcttc aaaccatcat
    20161 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    20221 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    20281 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatattgt gtatgcacac
    20341 ggatctgatg agtacgccct gttaccgggc tacaacgcac cagggtggca aatgcatagc
    20401 gctcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactcaca cgcacgatac
    20461 gttcgccgct ttgcagaata tttttcagta acggcgcttg aacgctcttc acatgcgact
    20521 gccactgacc gcgcaccagc gccaggtaat ctttttgcat ccctttttca cgtaattgct
    20581 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaggtgtccc
    20641 ggtcaagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    20701 cgataacgcc gaagcttaaa ccactaccgc catgtaccgc cgtaccggaa ggtttattca
    20761 gcaccaggat atggtcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    20821 gatgtggcga aaccgcctct tcttcccgtt cagcaacgcg aaccggtggg atacgcacct
    20881 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcgc
    20941 ctttacgcaa aatacggtaa atcatacttt ttggtacacc tttcaattgg gtacgcaaaa
    21001 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    21061 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    21121 tatgcttata tttagttttg caccttatta cttcactaca tgatcacttt attgatggtt
    21181 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    21241 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    21301 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    21361 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    21421 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    21481 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    21541 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    21601 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    21661 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    21721 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    21781 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    21841 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    21901 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    21961 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    22021 tatctggttc tgatgccgaa caacccgcgc gcgggtggta tttctcgtcg tatcgaaggc
    22081 gacgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    22141 cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc
    22201 ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    22261 ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgccaggac
    22321 atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct
    22381 gcattaggcc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    22441 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agttcgtctg
    22501 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacggccat cgacatcaac
    22561 tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg cgtttaacac caacctcgaa
    22621 gctgccgatg agattgctcg tcagttgcgc ctgcgtgacc tcggcggcct gattgttatc
    22681 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    22741 gcggtgcgtc aggaccgggc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    22801 gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    22861 cgctgctccg gtaccggtac cgttcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    22921 ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    22981 ccaattgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgcgat tgaaactcgt
    23041 caggacggcg tacgctgtgt gattgtgcca aacgatcaga tggaaacccc gcactaccat
    23101 gtgctgcgcg tgcgcaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg
    23161 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa
    23221 caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcaccaac gccagctgaa
    23281 cctgccgcgc ctgtcgtagc cccagcacct aaatctgcac cggcaacacc agccgctcct
    23341 gcccaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    23401 gaagaaacca aaccgtccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    23461 caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgaccct
    23521 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    23581 gcacagcagc agactgccga gacgcgtgag agccgtcagc aggcagaggt aacggaaaaa
    23641 gcgcgtacca ccgacgagca gcaaacgccg cgtcgtgaac gtagccgccg ccgtaacgat
    23701 gataaacgtc aggcgcaaca agaagtgaag gcgctgaatg ttgaagagca atctgttcag
    23761 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    23821 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    23881 gaagaaactg tcgcagccga accaattgtt caggaagcgg cagccccacg cacagaattg
    23941 gtgaaagttc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    24001 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    24061 cgcgtaagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    24121 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    24181 tatccaattg tacgtccgca agatgtacag gttgaagagc agcgcgaaca ggaagaagta
    24241 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgccgaacc tgttgttagc
    24301 gcgccagttg ttgaagaagt ggctgaagtc gtagaagccc ccgttcaggt tgccgaaccg
    24361 caaccggaag tggttgaaac gacgcatcct gaagtgattg ctgccgcggt aactgaacag
    24421 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagttgcaga acacgcagaa
    24481 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    24541 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    24601 acagcagaag ttgaaacggt agctgcggtt gaacctgagg tcaccgttga gcataaccac
    24661 gctaccgcgc cgatgacgcg cgctccggca ccggaatatg ttccggaagc accgcgtcac
    24721 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    24781 gcaacgcatc atgcatctgc cgctcctgcg cgtccgcaac ctgttgagta atagttagct
    24841 caagtaatca agccctggta actgccaggg cttttttatt tcatctttga gtcaacatat
    24901 taaaattcat caaatggcat ccttgctaac caacaataca aaataggcaa aaaaatgccc
    24961 gctccggcga tcccagagcg ggcagacaaa acatacccag tttcatgata tgttttaaag
    25021 cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac
    25081 gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca
    25141 tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc
    25201 agcgactcca gttcgttcag ttgcgtgcct aattccgcgc gaacagtcag cacattgttc
    25261 agcgagtttt tcagtccgcg gttggtttta tccagtgccg cagcggcggt ttctttatcc
    25321 gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa
    25381 agattggttt cagaagcgct accgtctggt tccgctaccg cgttgctagt aatactgtcg
    25441 aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgttta
    25501 atactttctg ctccgccttc gtatttgcca tccgcctcgc taaacggtgc ggattctgtt
    25561 ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac
    25621 tggtcacgaa gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg
    25681 ccattgctgg cgtagacaat tttttcctga gcattctgta tagcggttgt gacctggcta
    25741 agtacactct cttccagtga cactttttga gtggcgaaag tacgcgccag cgtgtactgg
    25801 ctgttttgcg cctgtgcctg ggagagaaca acagcttgtg atgcagcaat gggatcatct
    25861 gaagggttaa cgactcgctt acccgtcgac atctgttcgc cgtacttcat ccattctgcc
    25921 tgagaattgg tgatgccgcg catgttttgc tggtacatca tctgggtact gaaacgcatt
    25981 ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa
    26041 cctgcgcatt cgcgaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga
    26101 gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgag
    26161 tggtgctact ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat
    26221 aagcgtcgtt aaaggatttc gcaccgccca ccgttttact gttgctttgc agatcgagca
    26281 gggcctgacc gttgcggttg tcgctatcac ccgcatcttc tgcgctcgcc atggcgattt
    26341 tcgcttcgtc ggtgattaat acatccatgt tgacgatggc gtcacttacc ggtttcagcg
    26401 tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca
    26461 ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct
    26521 gccactgatt attatcgaac gagattttgt aatctgtcgc cagtaccgcg gaggtatcag
    26581 ttaccgttgc accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct
    26641 taccgatagc aaagaaatct tcaccggcat cgccgttagc atcaaatccg gctttgtgtt
    26701 gggtgttgaa agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt
    26761 ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct
    26821 ccgggatctc aatattgcct gccgtcccat caacataagc gacagtcgta cgagaagggt
    26881 cggcgctgga aggaactgcc gcaagttgcc gcgccgtact tccctgaacc agtgagtagc
    26941 cattggccat cgtgatgtta taagtgccac catcctgaac actgacttct acgccaacaa
    27001 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    27061 ctgcccccac gcctgtcagg cgggagatct gatcgttcag gctggcgatt tgtttagcgt
    27121 aattgttgat ctgatcaaca ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    27181 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    27241 cctggcgcgc tgccggatct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    27301 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    27361 tctgctcata gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt aactggttgg
    27421 taataaacgc atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    27481 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    27541 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    27601 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    27661 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    27721 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgaa tcatgttggt
    27781 gagtttgcgg gcatagtgag gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc
    27841 ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgcg ttaacagccc
    27901 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    27961 cttcgcttcg ccattttcat attcagtcgt ggtgatttca gtgactggcc ctttccagtt
    28021 gccagaggct ttgacaccaa acaggttata gctcggctcg ccgttttcac ggcggatttg
    28081 ccgttgtccc cagccagatt ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc
    28141 actttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc
    28201 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    28261 ctgattttga taacgcacca cggtttcgag cgggaatttt atcggtgctg ctggcgtgga
    28321 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg ccagccccag
    28381 acctttgccc gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg
    28441 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    28501 catctgcacg aacatccctt ccacctggcg ggccaccgga cggatatttg ccgctggatc
    28561 ttcgcccgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    28621 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    28681 tgcattgatt gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    28741 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    28801 cgtaaatcga tctgcgtttg tggagtaacc acagtctgcc caccaccaaa cggtgtatct
    28861 ggctggctga cattggcctg acgattaact gttactgaga gattcccctg cgctaccgcg
    28921 cagctgtcga gggtcacttc gcgattcatc accaccgaac cagtgcgcga gttaatcact
    28981 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg
    29041 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    29101 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catactgaaa
    29161 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    29221 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttgattcac ctgaacactg
    29281 ctaccgccag cggaggcccc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    29341 acctgactgt caacgccctt aagcggtgtc atcaataacg taccgccacg cagacttttg
    29401 gcatttccca tggaagaaac caccacgtca atggtttgcc cctgacgtcc aaacggtgga
    29461 agtgacgctg tcaccataac cgcagcgacg ttttttagct gcatattagt gcccgtcgga
    29521 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctga
    29581 gttgtctggt cacctgtacc atcaagcccc accaccaggc catagccaat cagtgagttt
    29641 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtggta
    29701 accagtaaaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    29761 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    29821 tgcctacgta ttcaatgcgc gcatctgcca cctgagtaga cggtacggta ttgctgccgc
    29881 tgatagtgcg tggattaacc accccagaga agcgaataaa ttcggtaccc tgattaatgg
    29941 cgatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    30001 ccgtcaacgt accgctaaag gtattgctgg cattggcccc gccttttccg ttgaacgtgt
    30061 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa caacccctgc aaatagcgcg
    30121 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    30181 tgctggcgct gacgttctcc tgcaacacga tagtcagcgt atcgccaata ttgcgtggtc
    30241 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    30301 acccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgtacca
    30361 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag cgccaacaag ctggaaatgg
    30421 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    30481 aacctgccca ccggttaagc cttagagttg cgtcagtttt tgcagcatct gatcggtggt
    30541 ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc
    30601 ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt
    30661 caggcccggc gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt agaggttttc
    30721 gccaatgctc tccagtccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg
    30781 aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat
    30841 acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cggccgtcac
    30901 cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgagccatc
    30961 tggcaacatc acctggaaaa agccctgccc tttaatcgcg acgtctttac tgttattggt
    31021 ttgcgacagg ttgccctggc tgtgtaaacg ttccgtggcg acagggcgta cccccgtacc
    31081 gatttgtaat ccggatggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat
    31141 ggtttgataa agtagatctt caaacaccgc gcgctgacgc ttaaaaccgt tggtactaac
    31201 gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt
    31261 ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac aaagtgcata
    31321 tgtcttcttg ccggatgcgg cgtaaacgcc ttatccggcc tacaagagct cgcaaaatcg
    31381 ataaattgcg attgccccca tagacccgat aaacttgcgc atagggcaac ttatacttgt
    31441 catgtatcct ttcaattaac tcatcgacag cagttggttg gcacggcctg cgttatcatc
    31501 gacgctgctg atcaccttca tctgcatttc aaaacgccgt gcgctggcaa tcatgtcgct
    31561 cattgccgca acggcattga cgttactgcc ttccagaacc cccgacatca cacgcaaggt
    31621 tggatctgcc tgcagtaccg gcccacgcgt agcctggctt tctgcgctta aacgaaaaat
    31681 gccatcgtca ccgcgctgga cttcgctgcc cgtggctttc accagcttca gacgccccac
    31741 tggcgcaacc gtatttgccg gatcgcccgg attcagcgcc gagattgtgc catcggcagc
    31801 aatagtgatt tccgcccctt ccggcacagc aattggcccc gcctcgccta ccaccggatg
    31861 tccctgaatt gtcagttgcc cggtgggatc aacctgaatg ctgccattac gcgtataccc
    31921 ttcactgccg tcagcggtct gcacgaccag ccaaccatcc tgctgcaacg caacgtccag
    31981 cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt
    32041 taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa
    32101 ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta ctggcggtta ccgcctgttg
    32161 attcagcgtc tggctggctg ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc
    32221 ccgtcagcga ttagcgtaag ttaaccagcg tattgaggat ctggtcctgg gttttgatgg
    32281 tctgggcgtt agactgatag ttacgctggg caacgatcat attgaccagt tctttactga
    32341 gatcgacgtt ggacgcttcc agcgcaccgt tggtcagggt gccaaagttg cccgtcccgg
    32401 ctgtccccaa cagcgccacg ccagaagatt gcgtagcgga ccagacgttg tcgccttcgg
    32461 atgccagacc ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc agttgggttt
    32521 gttcgttgga atagttgccg acaaccgtac cgtcatcatt gatttgataa ctcaccagat
    32581 cgcccggttt gtagccgttc tgggtggttg ccacaatgtt gttagcgccg gtattttgct
    32641 gcatggagtt gaggaagctc aggctaaacg tggcgggatc tgcgccgtta attgcgccgg
    32701 tggtaatatc ctttgttgga tctgaggtca gaacgccatt ggcattaaac accagcgtcg
    32761 ttgcagggtt agctgtgcct gttggatcac tgctatccag ggtgtaaaca tcccagttat
    32821 tatccccggt cttcacaaag tagacgctca tgtcatgagc attaccctga ctatcgaaaa
    32881 cagtcaccga accttttttg ttatagctat ccgcattgct ggcatcaaat gcgttaacag
    32941 agggaagcga atcactggaa ttcaggttga tctgcatcga cgccgtggtg gtagttttcg
    33001 ctgccatcag ggtattcgga atcgaaatat tggtcggatt cgccccttgc tgaatagtcg
    33061 gcggcgtacc ggttgccggg taacccgtca gctgtaaacc ttgcatattc accaggttac
    33121 ggttttcatc cagcttaaat tgtccgttac ggctgtagaa taccgaacca ttgctgtcta
    33181 ccagacggaa aaaaccgttc tggctgatag caacgtccag accacgcccg gtgttggtgg
    33241 tcgtgccatc ggtaaagtcc tgagtgatac cagcaacttt tacccccagt cccactttcg
    33301 aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag gtggcggagt
    33361 tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat ccgctaaccg
    33421 cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc tgccgtactt
    33481 cgtcgagggt agtggtgccg taagtgccaa gatccagcgt attaccgttg ttgccgcgga
    33541 tcaccccctg caccagagca aactgcagcg gttgggcaac cagttgtgta ccaccgttac
    33601 tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccgtca gtcaacgtac
    33661 cgtcccaggt gaaactgtgg actccggcag tcagttcacc gatatcaatg gtacggacga
    33721 ccgcgccgtt tttatcggtg atggtggccg tcactttgtc tgctgcctgt tgcaactcaa
    33781 caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc gcaagaactg
    33841 tggtaccggg gatcatcacg ccgtgaccga taaggttact ggcctgtaac gactgactgt
    33901 tatcaatctg tccggaaata gatccgagcg tggtattgag tttttcaatc ccactgaccg
    33961 tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg gtcgggtcct
    34021 ggtttttcag ctgcgccacc aacaaagtca gaaaactgct ttgtaaatct gcggcgttgc
    34081 tgcccgtgag cgaactactg ctggtggtac tgacgccggt attcgtcgga tcggtggtgg
    34141 ttaccgcaat agacatagct ttctccttta ttgaccgagc gtaagggttt tcagcatcat
    34201 gcttttcacc gtgttgagca cttcaacatt ggcctgataa ctgcgtgacg ccgacatggt
    34261 gttaaccatc tccccgacga catcaacgtt tggcattttc acgtagccct ttgcatccgc
    34321 cagcggatta cccggttcat aaaccagttt gtccggggcc tgactttcta taacatcggc
    34381 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    34441 tgcccgatat ggctgtccat cgggaccggt cacgctatca gcattcgcca gattactggc
    34501 cgccacgttc agacgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    34561 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt taatttgccc
    34621 gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc
    34681 gcgatccata tcgacagtat taccgtcaag cgaaggctgg tccggaatac ggtattgcag
    34741 ttctgcggaa ggaggcgtca gcgcctgcgc cggaatgtgt tgcgttgagg tcatcgtcag
    34801 agcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa
    34861 atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac
    34921 ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag
    34981 cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta aatgccttta
    35041 cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca
    35101 ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa
    35161 acgtagcgtg gcgatcatcg cgatactctt cagtccgtta agtacggcga gcaatctcac
    35221 atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt
    35281 ttctattcgt acagcgccca atctgctacc gccgtgcgag cagccattgc tttcgatgag
    35341 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    35401 atatttacag gttaatgtac aggccacagg aaattatgtg gttgccgcga tgcccattgt
    35461 gcgtggagga aagctggaag ctggcaatgt caaactgaaa cgcggacggc tggatacctt
    35521 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc tgcgcgatct
    35581 atcacccgat caacctatcc agttaaccca ctttcgccag gcatggcggg taaaagcggg
    35641 acaacgcgtc aacgttatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    35701 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atgatatcgg gacaagtggt
    35761 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    35821 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    35881 catgagtatt gatcgcactt cgcctctgaa gccagtaagc accgttcaac cgcgcgaaac
    35941 cactgacgcg ccggtaacaa acacccgagc ggcaaaaaca acagcctcca ccagcaccag
    36001 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    36061 tgaacgtgtc gaagcgttaa agctggcgat tcgtaacggt gaactaaaaa tggacactgg
    36121 caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta attgatcgta
    36181 tgacgcgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    36241 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    36301 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    36361 ggaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattgctg
    36421 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    36481 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctgtatg
    36541 gggcgaacgg tcagacctca accccctatc gcggcagtaa aaagatttcg atctgaagat
    36601 tatctccggc ctgcactgca ggccggaatg tatcgttaca ccgtccggcg ggcaaattct
    36661 ttaactttga agcccagtac tgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    36721 accgccatta aacgcagtaa acgccagggc atggtaccca atgaccactc cggcatgata
    36781 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    36841 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    36901 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    36961 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    37021 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    37081 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaag
    37141 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gaccggaaag aatgcccaac
    37201 gcaaccgcac ttggcagcgc caacaggaaa caaagacgca gcccccagtc catcaaacgg
    37261 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggtag caaaatggtg
    37321 ccaagcgcca cgcccagcac accggacgga aactccatta agcggtcggc gtaatacatc
    37381 cacgacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    37441 tggctcacag agacgccaag gattgccggt cccatctgtt tcaccacacg cattgctccg
    37501 gcatcgtgga agttaatgcg tggcaaaacc agcatgccga tcttcttcag atgaggtagc
    37561 tgatacacca gctgcaggat gccgccgacc gtaacagccc aggccagcgc cagcaccggt
    37621 ggattaaagt acggcgcggc aaacagcgca aaaccaatca tgctgatatt gagcaatgtc
    37681 ggggcaaacg ccggaattga gaagcggttc caggtattca gaatcgctcc caccagcgac
    37741 gccagggaga tcagcaagat atagggaaag gttatcttta gtagctggct ggtcagggca
    37801 aatttgtcgg cagtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcagcg
    37861 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    37921 acaaagaccc gcgtggcgtc ttcaccctgc ttgcttttat attctgccag aatcggtaca
    37981 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    38041 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    38101 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    38161 aataaattca tcggtgttct aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct
    38221 gaaagaggcg aatagtctac ctggattatg gtgaattgct accgccagat gttacagggt
    38281 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcacggcctg
    38341 ttcgccggct gtttgcggaa ctgtctgatt ttgcacgcat tcaatgaagt gccgcgcaca
    38401 gtcgacaaat ccacgttgct caagcgtact ctgccaacca ggaatcggtt tatgcactac
    38461 gccctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatca gcgcaccatc
    38521 agtcacggcc tgcacggttt cacgctgact tccggcccgg cgatgcatac aggtggtgat
    38581 ctgcaaagga ccagccgaaa aatggtgctc ggcaaacagc atttcgccgg actcattggt
    38641 cagcaacgta ccgctttcga gagttgcatt gccgccagac aaccacagcg cggtatcaac
    38701 cacatgcaaa taatcatcca gcaatgtgaa ataaagatcg tgtggcccga cgctattggt
    38761 acgatgttta tccattctta gcgaggacgc ggtggcgagt tgcgttttta actcaccgta
    38821 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    38881 ttccaccagc cgttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    38941 tacccccgca ttgagtaacg tactgaccac gccaaagtgg ctggcggtgc tggaatgcac
    39001 aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa tcagcataag gaatgcgcca
    39061 gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggcacctt gtaacgtcca
    39121 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcgatgccgc ctaaccccac
    39181 tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    39241 gctgtttcag ttctgccact tcgatttcca gggcttcgac gcgggcctgt aaatcaccgt
    39301 caaccgcgtt cgacatatcc gttaccgccg gcggattctc aacctcacca ctgaaaagat
    39361 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    39421 cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    39481 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacagcg
    39541 tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct gaattacaaa
    39601 aacgttgctc atatttggtg acccgattac caaaaccgct caccgtgcgt agataatgac
    39661 gtttgaccag attatccagc tgttcctgca cttcagattc actcagattc ataaccggtt
    39721 cacggttcgt tttctgatta caggccgtga ctacaccatt tacggagagc gggtattgct
    39781 ccggcgtggt cacctgtttt tccagcaagc aaccaatcac gcgggcttcc agggcggtca
    39841 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    39901 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    39961 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    40021 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc gctcagagct
    40081 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    40141 tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    40201 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatccct
    40261 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    40321 ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc acgatcatgc
    40381 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    40441 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    40501 acctgcgcaa ccagaccctg tgaaaagtcc agaatgccat tttctgcatt attcacatat
    40561 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat
    40621 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    40681 gaaatgtccc gcgtgtcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    40741 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    40801 caaatgggct gggccgccgt catggtcggt attgccctcg gtctacgcca gtttattcag
    40861 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    40921 gtcaccggta tgctgatgcg tgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    40981 tggctactgt ggttttcatg cctgctttcg ggactcggcg gcacgttgtt tgatcctccg
    41041 cgttcggcgc tggtggtgaa actaatccgt ccacaacagc gtggtcgttt tttctcgctg
    41101 ttgatgatgc aggacagtgc cggtgcggtc attggcgcat tgttggggag ctggctgttg
    41161 caatacgact ttcgcctggt ctgcgccaca ggggcagttt tgtttgtgct gtgtgcggcg
    41221 ttcaatgcgt ggttgttacc agcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    41281 ggcatgaccc gcgtgatgcg cgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    41341 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    41401 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    41461 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttaatggct
    41521 gggctgttga taatgtcatt aagcatgatg ccagtgggca tggtcagcgg cctgcaacaa
    41581 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    41641 accttaagtg cttcgctggc agacgcaaga gctcgcggca gctatatggg atttagccgt
    41701 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gcggctggct gtttgacctg
    41761 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggcattat tggcatcttc
    41821 actttccttg cgctgggttg gcagtttagc cagaaacgcg ccgcgcgtcg tttgcttgaa
    41881 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagaga actgacagcc
    41941 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    42001 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    42061 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    42121 agccgctata tgccagaaag catggacatc gttcactatg tcgataaact cgacggcaaa
    42181 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    42241 tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    42301 cctgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    42361 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttacg tgcgctggac
    42421 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    42481 tttccgctcc tgcgtaatct gacgctagta gccggaatca actggccaag ccgcgttgct
    42541 gattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    42601 taatcctgac ccgtcgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    42661 gctggcgcgt tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc
    42721 atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg
    42781 ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg
    42841 cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat
    42901 atcgttctga caaacctgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta
    42961 accggagacg ccaatctgga catgaactcc ctgttcggta gccagaaagc gaccatgaaa
    43021 ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gcgcgatctt cctgaaagag
    43081 atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg
    43141 cttccctatt taaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg
    43201 cgcgaagatg gcagccaggg cgaagcaatg gcgaagaaac tggcgaaagg cattgaagtg
    43261 aagccaggcg aaattgtcat tccgtttact gattaatcac gagggcgcat tcgcgccctt
    43321 tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct
    43381 tcagccggag catagagatt aatgactgca ccatcccagg ttttaaagat ccgccgccca
    43441 gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc
    43501 agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccaccgta
    43561 gaggctgccg tggcgtatcg ccagcgtatt cttgacgccg tacctgccgg gcacgatttc
    43621 accccgctga tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga
    43681 tttaacgaag gcgtgttcac cgctgcaaaa ctttatccgg caaatgcaac cactaactcc
    43741 agccacggcg ttacgtcagt tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc
    43801 ggtatgccgc tactggtgca tggtgaagta acacatgcag atatcgacat ttttgatcgt
    43861 gaagcgcgct ttatagaaag cgtgatggaa ccgctgcgcc agcgcctgac cgcgctgaaa
    43921 gtcgtttttg agcatatcac caccaaagac gctgccgact atgtccgtga cggaaatgaa
    43981 cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt
    44041 ggtggtgtgc gtccgcacct gtattgtcta cccatcctca aacgcaatat ccaccaacag
    44101 gcattgcgtg aactggtcgc cagcggtttt aatcgtgtat tcctcggaac ggattctgcg
    44161 ccacatgcac gtcatcgcaa agagagcagc tgcggctgcg cgggctgttt caacgcccca
    44221 accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctttgca gcactttgaa
    44281 gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc
    44341 gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga tgacacgctg
    44401 gtgccattcc tcgccggaga aacggtacgc tggtccgtta aataataaaa atcgtagctt
    44461 cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat
    44521 tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc
    44581 cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt
    44641 atcggtacgt tatgccgcag caaataattt atcggttatt ggcgcgacaa aagaagataa
    44701 acagcgcatt agcgaaattc tccaggagac gtgggaaagc gccgatgact ggtttgtcag
    44761 cgaataatat gcagtgattt ttgtttttgc cgggtcgccc cggctttttt atttt
//
LOCUS       DAPQZD010000018        43885 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPQZD010000018 DAPQZD010000000
VERSION     DAPQZD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.018"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(134..3283)
                     /locus_tag="RO454_001018"
     CDS             complement(134..3283)
                     /locus_tag="RO454_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDY0932888.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="RO454_001019"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="RO454_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDY0932889.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="RO454_001020"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="RO454_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDY0932890.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="RO454_001021"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="RO454_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDY0932891.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8817..8981)
                     /gene="rsmS"
                     /locus_tag="RO454_001022"
     CDS             complement(8817..8981)
                     /gene="rsmS"
                     /locus_tag="RO454_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDY0932892.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(8995..9522)
                     /gene="priC"
                     /locus_tag="RO454_001023"
     CDS             complement(8995..9522)
                     /gene="priC"
                     /locus_tag="RO454_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDY0932893.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9592..9969
                     /locus_tag="RO454_001024"
     CDS             9592..9969
                     /locus_tag="RO454_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDY0932894.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10122..10673
                     /gene="apt"
                     /locus_tag="RO454_001025"
     CDS             10122..10673
                     /gene="apt"
                     /locus_tag="RO454_001025"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDY0932895.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10802..12733
                     /gene="dnaX"
                     /locus_tag="RO454_001026"
     CDS             10802..12733
                     /gene="dnaX"
                     /locus_tag="RO454_001026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDY0932896.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12066..12130
                     /gene="dnaX"
                     /locus_tag="RO454_001026"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12786..13115
                     /locus_tag="RO454_001027"
     CDS             12786..13115
                     /locus_tag="RO454_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDY0932897.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13115..13720
                     /gene="recR"
                     /locus_tag="RO454_001028"
     CDS             13115..13720
                     /gene="recR"
                     /locus_tag="RO454_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDY0932898.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13830..15704
                     /gene="htpG"
                     /locus_tag="RO454_001029"
     CDS             13830..15704
                     /gene="htpG"
                     /locus_tag="RO454_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDY0932899.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWSVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15885..16529
                     /gene="adk"
                     /locus_tag="RO454_001030"
     CDS             15885..16529
                     /gene="adk"
                     /locus_tag="RO454_001030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDY0932900.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16661..17623
                     /gene="hemH"
                     /locus_tag="RO454_001031"
     CDS             16661..17623
                     /gene="hemH"
                     /locus_tag="RO454_001031"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDY0932901.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17620..18579)
                     /gene="aes"
                     /locus_tag="RO454_001032"
     CDS             complement(17620..18579)
                     /gene="aes"
                     /locus_tag="RO454_001032"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDY0932902.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18731..20035
                     /gene="gsk"
                     /locus_tag="RO454_001033"
     CDS             18731..20035
                     /gene="gsk"
                     /locus_tag="RO454_001033"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDY0932903.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20165..21841)
                     /locus_tag="RO454_001034"
     CDS             complement(20165..21841)
                     /locus_tag="RO454_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDY0932904.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22080..23300)
                     /gene="fsr"
                     /locus_tag="RO454_001035"
     CDS             complement(22080..23300)
                     /gene="fsr"
                     /locus_tag="RO454_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDY0932905.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23518..25170
                     /gene="ushA"
                     /locus_tag="RO454_001036"
     CDS             23518..25170
                     /gene="ushA"
                     /locus_tag="RO454_001036"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDY0932906.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25208..25711)
                     /locus_tag="RO454_001037"
     CDS             complement(25208..25711)
                     /locus_tag="RO454_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932907.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25708..26508)
                     /locus_tag="RO454_001038"
     CDS             complement(25708..26508)
                     /locus_tag="RO454_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932908.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26532..27011)
                     /gene="ybaK"
                     /locus_tag="RO454_001039"
     CDS             complement(26532..27011)
                     /gene="ybaK"
                     /locus_tag="RO454_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDY0932909.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(27215..28009)
                     /locus_tag="RO454_001040"
     CDS             complement(27215..28009)
                     /locus_tag="RO454_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDY0932910.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            28142..28444
                     /locus_tag="RO454_001041"
     CDS             28142..28444
                     /locus_tag="RO454_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDY0932911.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            28480..28821
                     /locus_tag="RO454_001042"
     CDS             28480..28821
                     /locus_tag="RO454_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDY0932912.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(28879..31383)
                     /gene="copA"
                     /locus_tag="RO454_001043"
     CDS             complement(28879..31383)
                     /gene="copA"
                     /locus_tag="RO454_001043"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDY0932913.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            31645..32577
                     /gene="glsA"
                     /locus_tag="RO454_001044"
     CDS             31645..32577
                     /gene="glsA"
                     /locus_tag="RO454_001044"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDY0932914.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            32580..33872
                     /locus_tag="RO454_001045"
     CDS             32580..33872
                     /locus_tag="RO454_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDY0932915.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVSTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            33997..34404
                     /gene="cueR"
                     /locus_tag="RO454_001046"
     CDS             33997..34404
                     /gene="cueR"
                     /locus_tag="RO454_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDY0932916.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(34405..35140)
                     /locus_tag="RO454_001047"
                     /pseudo
     CDS             complement(34405..35140)
                     /locus_tag="RO454_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35234..35692)
                     /locus_tag="RO454_001048"
     CDS             complement(35234..35692)
                     /locus_tag="RO454_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDY0932917.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(35689..36606)
                     /locus_tag="RO454_001049"
     CDS             complement(35689..36606)
                     /locus_tag="RO454_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDY0932918.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36752..37429
                     /gene="fetA"
                     /locus_tag="RO454_001050"
     CDS             36752..37429
                     /gene="fetA"
                     /locus_tag="RO454_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDY0932919.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            37416..38195
                     /gene="fetB"
                     /locus_tag="RO454_001051"
     CDS             37416..38195
                     /gene="fetB"
                     /locus_tag="RO454_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDY0932920.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(38378..39232)
                     /gene="cnoX"
                     /locus_tag="RO454_001052"
     CDS             complement(38378..39232)
                     /gene="cnoX"
                     /locus_tag="RO454_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDY0932921.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(39293..40102)
                     /gene="ybbO"
                     /locus_tag="RO454_001053"
     CDS             complement(39293..40102)
                     /gene="ybbO"
                     /locus_tag="RO454_001053"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDY0932922.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAVMVLKRLLPGRVMDKILQG"
     gene            complement(40092..40715)
                     /gene="tesA"
                     /locus_tag="RO454_001054"
     CDS             complement(40092..40715)
                     /gene="tesA"
                     /locus_tag="RO454_001054"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDY0932923.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            40686..41372
                     /locus_tag="RO454_001055"
     CDS             40686..41372
                     /locus_tag="RO454_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0932924.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            41369..43783
                     /locus_tag="RO454_001056"
     CDS             41369..43783
                     /locus_tag="RO454_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932925.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT        10553 a        11465 c        11598 g        10269 t
ORIGIN      
        1 ttttatgaat tttatacgtg gttaatactg gttttcgtat gggatcctga gttggtggtt
       61 caatacatcc ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg
      121 attacacgtt gtatcaatga tgatcgacag tatggttgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccaacgag gtcatcagga
      361 ttgggcgtaa acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaaaccaa
      481 tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc
      601 agctctcgta cagcgcagcc agacacagga acacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tagatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaacggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcctctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcggtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcacgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaattcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgta
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ccaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtggcttct tttggtggca aaccttcttc cgccatcaca cgctcaacgt
     2041 tttctaccac aacgatggcg tcatccacca acaggccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcaag aacagataca
     2221 ttaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggtgtggtg tcatacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg ttcgcaccgg
     2401 tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca attttcgcca catcacgcag cagcacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgaccagctg
     2641 caacctgggc gttctgtgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagttc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 cgtcacccac gcccgacgta cggctgatgg catctttcat attcgccgcc acgtagtcgg
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgtac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccagggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaattggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc gatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caaccaccag tacggtggca
     3541 tcgccacgtg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcacg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg
     3841 ctggactggg tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgtttg acttaccaat gcgaccacta
     3961 atcggagagg tgactttggt gtaagccaga ttgattcgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgt
     4081 ttactgatgt actgagtacc gagcaatttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgc cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca cctgcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactcg
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgagtccacg gtgacagaag aacggcgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgcgcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaac cctgccacta
     5281 acgaataacc ctgaatctga ctccaggaat tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcacgggcg
     5521 tcatcgaatg gtgatttacc gacaaaagcg gacctgcagg cacaacttga ctcactgaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcgtaaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagtgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agcgcgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa atgatctggc gtcttataac
     5881 agccaactgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gtccagcagg ctttgctgaa tgccgagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac actgtcttgc aggatacctt gcaaaagcag
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttacaact gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg cgcaggaagc tgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaagc aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact caaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgtgcg ctgcaatcgg aacgtaatat taaggagcag
     6421 attgccgttc tgaagggcag tctgctgttg tcacgtatcc tgtaccagca acaacaaaca
     6481 ctcccctcgg cagatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag tcaaccagca gcgtgacgcg ctgttccaga gcgatgcgtt cgtcagcaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcatt attgcaagta
     6661 gttgatatgc gtcgcgaatt gctggatcag cttaacaaac agttgggtaa tcagctgatg
     6721 atggccatta acctgcaaat taaccagcag cagttaatga gcgtatcgaa aaatctgaaa
     6781 tccatcctga cgcagcaaat tttttgggtg aacagtaacc gcccgatgga ctgggactgg
     6841 attaaagcgt tcccgcaaac gctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcccgc cgtattcatc gccttcctcg ctggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca aaaactggct
     7021 tccgctgtgg gttccctgcg taacgacagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcactgcc ggtgtgcctg attattctcg ctgtcggcct gattctgctg
     7141 actatgcagc tcaatatcag cgagttattg tggtcgttca gcaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaagtg ctggagaaaa atggtgttgc cgtgcgtcac
     7261 tttggtatgc cggaacagca gaccagccac tggcgtcggc aaattgtccg catcagcctc
     7321 gcgttgctgc cgatccattt ctggtctgtg gtggcagaac tctccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggtg
     7441 tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7501 attaccgtgc tgtcgattat cccgattgcg ttgatggtgc tgaccgccac gggttacttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatttggt gatcatctgg
     7621 aacctgctgt accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgccgtgcac tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc gctggagcag gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtctga tttgatcacc
     7861 gtgttcagct atctcgacag cattacgctc tggcattaca acgctactga agctggtgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag tgctggtgct ctcgcgactg
     8041 aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt gggataaact ccagtggctg
     8161 gcggcagcat tatccgtagg tcttggtttt ggtttacaag agatcttcgg taacttcgtt
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgcattg gcgacacggt aaccattggt
     8281 agcttctcgg gtacggtcag caagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgattatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca cgactacgcg tctggtgatc cgtctcggcg tagcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatt
     8641 gatcagttgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga agtgacggaa gtgaaacgcg actacaaagg cgacgatccg
     8761 acgccagcgg tagggtaagg acaataaagg gcgacagtga tgccgccctt tattttttac
     8821 tcgctttgtg aagtctcatc gctctgtaac ttcttttcat aatcgcgttt tacaatatcc
     8881 agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg
     8941 acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc attgctagcg
     9001 ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcaac gtgccaggcg
     9061 cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat cggtcgctcg
     9121 cgccagacgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg
     9181 ctgaatacgt ttacgctgcc agcgggcaat tttcggtggt gcgctatccc actcgcgtag
     9241 cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat gttctgccag
     9301 ccaggcgact tgcggcagct gttgctgctc aaccgcatga cgaagcgcag ccaggttatc
     9361 gcccgactca tcgagacaag cctgtagtgt tgtcgcacgg gtctgaaaaa ggtgcctgtc
     9421 aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac gcagcgtggc
     9481 gagctgtcct tccagttttt ccagtagcag ggcggttttc acgataagac ctcaatgaaa
     9541 atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga
     9601 atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta
     9661 ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc
     9721 ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg
     9781 cagaaacatc atgcgatgcc gcgcggcgca aaaccacggg cgattttgct tattttgatt
     9841 acgtttacga tttcgctgtg gttcgtccag atgccgtggg tgcgcatcat gttgctggta
     9901 attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga aaagcaagaa
     9961 aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg
    10021 ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgttcaa
    10081 aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc
    10141 agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc
    10201 ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact
    10261 tgctggttga gcgttacaaa aatgcgggca ttaccaaagt ggtcggcacc gaagcgcgtg
    10321 gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta
    10381 aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg
    10441 atcaactgga aatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg
    10501 atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg
    10561 gtgaagtagc tgacgctgcg ttcattatca acctgttcga tctcggcggt gaacagcgtc
    10621 tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgt
    10681 cagtcttgcg ctgctcacgc tacgggcagc acaagatgtg cattcagcct cgccgttctg
    10741 acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc
    10801 aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg
    10861 ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc
    10921 ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa
    10981 ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg
    11041 tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgtaccaa
    11101 agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt
    11161 caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta acgcactgtt
    11221 aaaaaccctt gaagagccgc cagagcacgt taagtttttg ctggcgacga ccgatccgca
    11281 gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaagg cgctggatgt
    11341 cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc
    11401 gcgggcgctg caattgttgg cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct
    11461 gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggggg tcagtgcgat
    11521 gctgggtacg cttgacgacg atcaggcgtt atcgctggtt gaagcgatgg tcgaggccaa
    11581 cggcgagcgc gtaatggcgc taattaatga agctgctgcc cgtggtatcg agtgggaagc
    11641 gttgctggtg gaaatgctcg gtctgttgca tcgtattgcg atggtacaac tttcgcctgc
    11701 tgcacttggc aacgacatgg ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat
    11761 accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc
    11821 gtatgcgccg gaccgccgta tgggcgttga gatgacgctg ctgcgcgcac tggcgttcca
    11881 tccgcgtatg cctctgcctg atccagaagt gccacgccag tcctttgcgc ccgtcgcgcc
    11941 aacggcagta atgacgccaa cacaggtgcc gccgcaaccg caatcagcgc cgcagcaggc
    12001 accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca
    12061 gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg
    12121 gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg
    12181 cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc
    12241 gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc
    12301 gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag ccattgagcg
    12361 cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg aacaggtggc
    12421 gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc gctcctctca
    12481 gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga gtacgttcaa
    12541 aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc gtacgccgct
    12601 ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc
    12661 ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg aagaaagtat
    12721 ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga ttgagagaga
    12781 aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag cagatgcaag
    12841 aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc gaatctggcg
    12901 caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta gagatcgacc
    12961 cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca gcattcaacg
    13021 acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa
    13081 tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc tgttaacaca
    13141 gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc agcgtatggc
    13201 gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg
    13261 ggcgatgtcg gaaatcggcc actgcgccga ttgtcgcacc ttcaccgaac aggaagtctg
    13321 taacatctgt tcgaatccac gtcgtcagga aaacggtcaa atctgcgtgg tggagagtcc
    13381 ggcggacatt tacgccatcg agcagacggg gcagttttca ggtcgttatt ttgtgttgat
    13441 gggacacctg tcaccgctgg acggcatcgg tcctgatgat atcgggcttg atcgtctgga
    13501 acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc ccacggttga
    13561 aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg tggaagccag
    13621 ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg gcaccacgct
    13681 gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga gcaggatcac
    13741 ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct gtttttaacc
    13801 ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt ggttttcagt
    13861 cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat aaagaaatct
    13921 tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt ttccgcgcgc
    13981 tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc tctttcgata
    14041 aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc gacgaagtga
    14101 ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa tccctgggtt
    14161 ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc tactctgcgt
    14221 ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg
    14281 gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc actaaagaag
    14341 atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc ctcgatgact
    14401 ggagtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg gtagagatcg
    14461 aaaaacgcga agagaaagac ggcgaaacca ttatctcctg ggagaaaatc aacaaagcgc
    14521 aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa gagttctaca
    14581 aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc gttgaaggta
    14641 agcaggagta caccagcctg ctgtacattc cgtctcaggc accgtgggat atgtggaacc
    14701 gcgatcataa acacggtctg aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag
    14761 aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc
    14821 cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac ctgcgcaatg
    14881 cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac gcggaaaaat
    14941 accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg gaagatttcg
    15001 ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tacccatacc gattcttctg
    15061 cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag gagaaaatct
    15121 actatatcac cgcagacagc tacgcggcag cgaagagcag cccgcacctg gaactgctgc
    15181 gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact
    15241 atctgactga gttcgacggt aaaccgttcc agtctgtgtc taaagttgac gagtcgctgg
    15301 aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca ctgactccgt
    15361 tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc
    15421 gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc actcagatgg
    15481 cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc ttcgaactga
    15541 acccggatca cgtactggtg aaacgtgcgg cggatactga agatgaagcg aagttcagcg
    15601 agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggcacg ctggaagatc
    15661 cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat gccggatgac
    15721 cttcgtgtca tccggcattt ttcttttctt catctgcact ttccgcaaat tatcttgcca
    15781 ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg gtatcgttta
    15841 tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt atcattctgc
    15901 ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag aaatatggta
    15961 ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc tccgagctgg
    16021 gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa ctggtgatcg
    16081 cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg ttggacggct
    16141 tcccgcgtac cattccgcag gcagacgcaa tgaaagaagc gggcatcaat gttgattacg
    16201 ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc cgccgcgttc
    16261 acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta gaaggcaaag
    16321 acgacgttac cggtgaagag ctgactaccc gtaaagacga tcaggaagag accgtacgta
    16381 aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac tccaaagaag
    16441 cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg gctgaagttc
    16501 gcgctgatct ggaaaaaatc ctcggctaat caagccgcag cagatctgct ttcgggcagg
    16561 tctgctcatc ctcctcaggc aatacctctt acagcaatta gttcttcttc ttcacttttc
    16621 cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg
    16681 tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg taaaacgcta
    16741 tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt tatggtggcc
    16801 gttgctgcgc ggcgtgattt tgccgctgcg ttcgccgcgt gtggcgaagc tgtatgcctc
    16861 tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac agcaggcgct
    16921 ggcgcaacgt ttaccggaga cgcccgtggc gctgggaatg agctacggct cgccatcact
    16981 ggaaagcgcc gtagatgaac ttctggcaga gcatgtagat catattgtgg tgctgccgct
    17041 ttatccgcaa tactcctgtt caacggtcgg tgcggtatgg gatgaactgg cacgcattct
    17101 ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg ataaccacga
    17161 ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg gcgaaccgga
    17221 tctgctgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag gcgatgatta
    17281 cccgcaacgt tgccgcacaa caactcgcga actcgcttcc gcactggaga tggcaccgga
    17341 aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga tgccttatac
    17401 cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtgggt catattcagg tgatgtgccc
    17461 gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa accgtgaagt
    17521 cttcctcggc gctggcggga aaaaatacga atatattccg gcgcttaatg ctacaccgga
    17581 gcatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa
    17641 ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa
    17701 ggcgtgcagc gtgcctgggt agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt
    17761 ctggtaaagc agatggctgt catccagcag cggatcgaac tccgccccgg caataaaaca
    17821 gggcggaact tcgcgagtga gatcattatt aaacaggcag taatatggcg actcgcggtc
    17881 cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg ttaagccatc
    17941 ccagacacca cccaacagac gacgagccac ggaatcccgt aatccgtaaa gcccatacca
    18001 cagcaaaacg cccgcaactt taccgcaatc gatctgttta tcgcgcaacc acaacgcact
    18061 ggcgagcgcc agcatggcac ctgcggaatc accggcaaaa ccaatgcggg acatattgat
    18121 ttgataatcc tccgcctgct ggtggaaata acaacaggca gccacaattt cctctatcgc
    18181 ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaatg ccaatcaccg tacattggct
    18241 gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga gaataaaacc
    18301 gcctccatgc aaataaaata gcgtcgccgg gctatctggc tgcggataaa agatacgtgt
    18361 tttcacctgc ccatattttg ttggaaccct gtaagctctg gttgccattt ctggagcgtc
    18421 cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa tcgctcccgt
    18481 tgcaggccag ggcggtaaat ccggctgaag agtattcaca acggtcttca tttcagcaga
    18541 gataaggtcc agaacgggta atttgttttc cggcttcatg caaaaactcc tttcaaatta
    18601 cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg
    18661 ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt aaccacccgt
    18721 aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc ccgtaaacgc
    18781 acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgctg cctgggtagt
    18841 gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat tcattgagcg
    18901 ttatggatta agcgccgggc attcactggt gattgaggat gacgttgccg aagcgcttta
    18961 tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca ccattggtaa
    19021 caccatgcac aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg
    19081 cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacacct ccagccgtac
    19141 cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta cgctgattgg
    19201 cgagtccggg gaacgtacct ttgctatcag cccgggccac atgaaccagc tgcgggctga
    19261 aagtattccg gaagatgtga ttgccggagc ctcggcactg gttctcactt cttatctggt
    19321 gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagccattg agtacgcgaa
    19381 gaaatataac gtaccggtgg tgctgacgct gggcaccaag tttgtcattg ccgagaatcc
    19441 gcagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga acgaagatga
    19501 agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg cgctggactg
    19561 ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg gctttaccga
    19621 agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggggctatag cggaatttaa
    19681 tcagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc tgcgtgtcta
    19741 ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta atggagcagg
    19801 ggatggcgca ctggcagcgt tgctgcatga cattaccgcc aacagctacc accgtagcaa
    19861 cgtaccaaac tccagcaaac ataaattcac ctggttgacg tattcgtcat tagcgcaggt
    19921 gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac ctcgtttaac
    19981 gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc gttaagttat
    20041 cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcattttgc tctcaatgcc
    20101 tgatgcgacg ctggcgcgtc ttatcaggcc tacatatccg cgcctattat ccggcaacca
    20161 ccgtttacct cgtcaccacc tcacccgccg gtggcgtttc cagcagttcc agcatagtac
    20221 gggcaatttc acgctcaccc atcactacct gattcgcacc acgttcggtg atatacgcca
    20281 cttcatcgtc ataatgggcg cgggcaataa tctcaatatc cggatttttc gcgcgggcag
    20341 atgccacaat ctcgcccgct tcatagccgt tgggaatcgt caggatcagc cattttgcac
    20401 attccagatg cgccagttgc ataatttctt cgttagccgc attgcccaat actgcgcgga
    20461 ccccgcgctc gcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa
    20521 tatcagaggc gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccagta
    20581 gcgcatggtt gcaaatatcc actgggatct gcttctcttc ttcgattgcc tcttccagcg
    20641 tctgctcttc cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg
    20701 ggttgagcat aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca
    20761 gtaaattcaa tgccattcct agtcccgcca ggataaacgc gaactcacca atctgcgcca
    20821 ggctggcggc gatggttaac gccgtgcgct gggagtgacc aaacaaacgc acaaggaaca
    20881 gggccgcgac cgatttacca aacagaataa ttgccagcgt cgccagtact gccagcggtt
    20941 gctgaatcag aattaacgga tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa
    21001 acgcgtcgcg caatggcagt gtatcgtggg cggcacggtg actcagttca gactcgttca
    21061 gtaccatccc ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac
    21121 caaaggcaat ccctaacgcc agcgccagca ccgacagggt aaacagctcg cgggaaccgg
    21181 ttgctgcgct gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg
    21241 cgataaatgc gatcaccttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc
    21301 ccacatcgcc ctgttccatc attcctgcta ccgcgggtag caataccagt gtcaggacca
    21361 tcaccaggtc ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtcaatta
    21421 attgccgttc ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataagc
    21481 cgaacacgat accggtcatt aacgaccagc ccagcacggc ggagagcgcc atacccagca
    21541 gcgtcgccac ggctatctgg gcgatcgcgc cgggaatggc gatggacttt accgccatca
    21601 aatccttgag cgaaaaatgc aaaccgacgc cgaacatcag caaaataacg cccagttcag
    21661 ccagttccgg cgcaagctta gtatcggcaa caaagcccgg agtgaatggt cctgccagca
    21721 cacccgctaa cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga
    21781 ggataaaggc gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca
    21841 ttccgtctcc ttttcctggt ggttattgtc catttttggc cgggaaaacc aaaattacag
    21901 gtaatagttt atgacaattt cattgatgat gtttatgaat aattgttgaa ttttgcagaa
    21961 aaatggaatt agctgcaaaa aaaagcacgg atcggaaaac agaaggcggt ttaacagagg
    22021 aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct gcaatgaaat
    22081 cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag tggcaggaaa
    22141 gcacagattt tatagactaa ctctatgctg gtgtggtcgg cgataagccc cagaactgcc
    22201 gcccccagac ctcccatgcc gaaagcaaaa ccgaaaaata gtccagaaac cataccgata
    22261 cgtcccggaa gcagctcctg ggcatagacc agaatggcag agaatgccga agcgaggata
    22321 aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg taaaatcagc
    22381 gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgtcc aattttatcc
    22441 cctacaggcc cgccaatcac cgtacctgcc gcaacggcaa acaggaaggc aaacaaatga
    22501 agctgagcat tctggataga taatccgaat ttttgcatca gataaaaggt gtaatagctg
    22561 ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat gctgaccgcc
    22621 agaacaactt tattgcgcgg cagtggattg ataatcgtcg ctttgggttt tcctttattc
    22681 attcggtgct gtgccgagta ccaacggctg atttgcgcca acaccacgat cgccagcagt
    22741 gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat caccgccgcc
    22801 agcaaggggc ccagggaact gccaaagttg ccgccgacct gaaatataga ttgcgccagg
    22861 ccatgacgcc cgccggaagc catacgggcc acgcgagaag attccggatg aaagaccgat
    22921 gaaccggtac cgaccagcgc cgctgccagc agaactgcgc cgaaactgcc cgccagcgcc
    22981 agcagcacca gaccacttaa ggtaaagcac atgccaatcg gcaacgacca cggcatcgga
    23041 tatttatcgg tccagtagcc gaccaccggt tgcagtagcg aagaggcgag ctggaaggtg
    23101 agggttatca tgccaatctg cataaatgtc agagaaaatt ctgactgaag cagtggataa
    23161 atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct gatagcacct
    23221 aaaataccaa acgatgttcg ggccttagtc gttgacgcag ccgcgcccgc cacaggctgg
    23281 gtttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt aaagaatgat
    23341 cttatttgtg attattaccc gactaacata cctgtatgcg ccgcctgaag gaagtctcaa
    23401 cacggaatac agaatttcta atctggatgc agatttatct tcaccggacg cagacttgtt
    23461 tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga gagaagtatg
    23521 aaattattgc agcggggcgt ggcgttagca ctgttaacca catttacact ggcgagtgaa
    23581 actgctctgg cgtacgagca ggataaaacc tacaaaatta cagttctgca caccaatgat
    23641 catcatgggc atttttggcg caatgaatat ggtgaatatg gtctggcggc gcaaaaaacg
    23701 ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct gctactttcc
    23761 ggtggcgaca ttaacactgg cgtgcccgag tccgacttac aggatgccga acctgatttt
    23821 cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga atttgataac
    23881 ccgctcaccg tattacgcca gcaggaaaag tgggctaagt tcccgttgct ttccgccaat
    23941 atctaccaga aaagtaccgg cgagcgcctg tttaaaccat gggcgctgtt taagcgtcag
    24001 gatctgaaaa ttgccgttat tggcctgacg acggatgaca cagccaaaat tggtaatccg
    24061 gaatatttca ctgatatcga atttcgtaag cccgctgatg aagcgaagct ggtgattcag
    24121 gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat ggggcattac
    24181 gacaatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct
    24241 gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg tatggcggca
    24301 gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccgga tcaacaaaac
    24361 ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc tgatttcgag
    24421 tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa cctgaagaag
    24481 aaagtgacct gggaagacgg gaaaagcgag cgcgtgctgt acacccctga aatcgctgaa
    24541 aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc gcagctggaa
    24601 gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta
    24661 cagaccaata tggggcggtt gattctggca gcacaaatgg atcgcactgg tgccgacttt
    24721 gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggtgatat cagctataaa
    24781 aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat gaccggtaaa
    24841 gaggtgattg attacctgac agccgtcgca caaatgaagc cagattcagg tgcctacccg
    24901 caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct gaaaatcaaa
    24961 ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga cattaaactt caatgccacc
    25021 ggcggtgatg gctatccgcg ccttgataac aaaccgggct atgtgaatac cggctttatt
    25081 gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt gagtgtttat
    25141 gaaccgaaag gtgaggtgag ctggcagtaa tgaaccccgt cgcggcgtca tgatatgacg
    25201 ccgccattta tgaatattga aatcttgcat cgtaatcgcg atgtgtcaaa ccaaatgcca
    25261 tcagcatata attctgcgga tcaccaggct gttcttcatc tgcttcaaac caaagtgtca
    25321 cgcctgagcg attgacgtgg attaagagtg tatggggcga ttgacggcgg atttgcagat
    25381 cagcgccttc gttaacaagc atgttaaata cacgcgtaac gttcaactca gcgacgttat
    25441 tcaatattcc tttatcaaat cggatcgctt tgcccaaatg gatttggctc agataatcgc
    25501 accaaatcat atatagcttg ttgttttcga aatgaaactc aacattacca aatagccaga
    25561 tataaaaacc atttcccata tcatttttag agtccggctc aacaaagttt tgttccaacc
    25621 atgccattgt ttgccccagt tcgacaaaat cgaattttcc gttaaagaca aaattgtgga
    25681 agctaacatc aacaagatgg tcaaatttca tctttcgctc ctttgtgaat tttttacatg
    25741 cttcaaaagc cagcgtaatt tacatcggtg caggcgactc tccagtgttg agcgaaaatt
    25801 tgcgatgtcg acattggacc gatctaccgc agcaaccccc gtgtattgtg ggatcggtga
    25861 agggattttt tgtaactttc ccagcgccag cagttgttga atgatcctgc taccgtcgtg
    25921 gtaatcatcg taaatagcca ctggcagagg ctgccaggcg ggggtcgatt tgataaatcc
    25981 ggaaaaaaaa tacgggtcca gatcgccgaa atcaaagccg taggcgaggg gaacttcaat
    26041 attttgctct gtttttttat tctcaaatgt acaatgaaaa ccgtgaaaac aatactgcca
    26101 ctttcccatt ttgccttttc gcttacggtt gcatttgaag ggggcaaagg taactagcgg
    26161 gttattttcc gccatctcaa cgttcagttg ttcagccaat gcaaatataa gctctgtcgc
    26221 cagcttacga tattctttcg cgcagtgttc gaaaaaatcc tgatggcggt taatctcttc
    26281 aagcgatatt aatctggcga tcctgataac cgggtcggca gaatgcatgc caacaacaaa
    26341 ttcatcgagc tgtgctttca gcaccagccc gaattcctca cgatacctct ctgcttcgct
    26401 ggcaaaatgc agatgtaata ccttctctct cgatgtttgc cgaagtaagg cgatatccgg
    26461 tatctcatct gcacaagcga tacttaatgt ctctgtaatc ctttccacat ctcatcttcc
    26521 gtgtgcatca cttaatcgcg acgggcgata tcagcaaatt tggcatcgag tatctttgcc
    26581 aggtcgcttg ccgccagttc gatatccagt ccgcgcttgc cgccggaaac ataaatagtg
    26641 gcaaattctt gtgcgggggc gtcgataatc gttggcagac gttttttctg ccccagtggg
    26701 ctaatccccc ctaccagata ccccgtcgaa cgctgcgcga ccatcggatc ggccatctca
    26761 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
    26821 gtaacagcca cggcaaggtg tttcatatca ccgttcactg cgaccagcag cgttttgtag
    26881 acctgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagca
    26941 ggatcgtgct cgtaggtgtg gatttgaaac gaaatcttgt ttttttcgag taatttaact
    27001 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
    27061 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
    27121 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
    27181 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatctgc ggcaaattcc
    27241 cttctccata caggtgtagt gcaccgaccg cgaccacata gcgccccggc ggcatggcgc
    27301 gtaatttgtc ccgccaggcg aggtttcgct gatgcatcag cacatcgtac agcgactgac
    27361 tgaacgtatt gggcagcgtt atatcattat tttgcggcgg tgcattcagc caccagctca
    27421 tcatttgttg cagcaaccgt gcgttggtgt gccagtgggt cagcgtatcg tccagcagcg
    27481 ccagtccttt gtcagggagc tggagcaaca tggcaatctg gttttcagcc ccttccagtt
    27541 caatcacggg tttatgttgt tgcttagccg cctgcaatag ctggtaatcg ataccgtatt
    27601 ctgcccgcag ccccagtttt tgcgcctgcg tcgcctgaag aaccatcgcg atttgccaca
    27661 gcggttgggt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcaggt
    27721 tttggagttg gtcctcgcta atgcgctctt ccagggcctc gcaggcaggc aaattggcaa
    27781 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
    27841 ttttgagcaa acgggtgggc aggggagcca tatcgtggct acccatatga atactgccga
    27901 tcagatgaaa atggcgattg ccgggaagag agatatcgat agcaggccag gtgtaatgat
    27961 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
    28021 ttgtgaaata tcatgctagc gcgcggggag ggatggcgca aatggagttg attgttgcac
    28081 ttgtgcacag tgcttgtgtg cattacaatg attaaataat ggacaaggag atgagagagg
    28141 aatggctcaa aagaaaaata taaggagttt tcgtgatgcc tggttggctg atttttttgt
    28201 ccattcaacg ccacatcgaa agatccctgc tgaaatccat accacgttat cacgaaagtt
    28261 ggacatcatt aatgctgcca cttctcatcg ggatttacgc tcccctccgg ggaaccgata
    28321 cgaagagtta tccgggaaat tacaggaata ctcctccatt agagtgaata aacagtaccg
    28381 actgattttt aaatgggtta atggcaaggc cgaagaattg tttttagacc cacacaacta
    28441 ctaaaacata gctgtcgtat tgacgagaaa ggtaaaacta tgaaacaggc aacaagaaaa
    28501 ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga tttgaaaatc
    28561 aatgagttag cagagttgct gcatgttcat cgtaatagcg tcagtgcact gatcaataac
    28621 aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga taccacagtc
    28681 gatttttggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa caacatgcgt
    28741 acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg ccgtgaagag
    28801 cgtgcaaaaa aggtcgcgta gaataatttc cctataacgg atgcgccacc ctaaagcagc
    28861 gcatccgcaa tgatgtactt attccttcgg tttaaaccgc agcaatcggt tggcgttact
    28921 cactacggtg atcgacgaga gcgccattgc cgctccggca accaccgggt taagcagtgt
    28981 tccagtgaac ggccacaaaa taccggcggc gaccggaata ccaatgctgt tgtagataaa
    29041 cgcaccgagc agattctgct tcatgttgcg cagcgttgcg cgggaaattg caagcgcatc
    29101 cgcaacaccc atcaggctat ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc
    29161 actgccgcca cccatcgcaa taccgacatc cgcctgagcc agcgctggcg cgtcgttaat
    29221 gccgtcgcct accattgcca cctgacgtcc ttcactttgc agacgtttga tcgcttcggc
    29281 tttaccgtct ggcagcacgc cggctatcac ctcatcaatc cctgcttctt tggcgatcgc
    29341 attggcggtg gtcgggttat ccccggttag catcaccagg cgatatcccg ctttatgcag
    29401 gcgttgcagc gccgccacgc tatcactacg caacggatcg cgtacagcca gcagggctac
    29461 cgctttcccg tcaaccgcca gcagcacagg cgttgcccct tgcgatgcct gagctgaaat
    29521 atctgcttca attgctttgg tatcgacctg ttggtcattt aacagcgcct gattgcccag
    29581 caataacgca tgaccttcag cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc
    29641 gttgacctgc ggtagctgca tatcacctgc tttatcgagg atcgcccgtg ccagagggtg
    29701 gctggaacct tgctccagcg ccgccgccag acgcaatgct tgcgcttcat caatatcagc
    29761 aaatgttttt actgcgacaa cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa
    29821 cactacggtg tcgagcgtac tggcgcgttg cagcgcgtcg gcgtcccgta ccagcacgcc
    29881 aaactcagcc gcccgcccga cgcccgaaat aatcgacatc ggcgtcgcca gccccagcgc
    29941 acacggacag gcaataatca gcaccgtggt ggcaatcacc agggtataga caatctgcgg
    30001 tgccggacca aagaaatacc agattgccgc actgatcagc gcaatcacca ccactaccgg
    30061 cacaaatacg gctgagattt tatccgccag ctgaccgatt tctggcttac tgctctgggc
    30121 ctggcgcacc atacgaataa tccgcgacag cgtagtatgg ctgccaaccg cactggcgcg
    30181 aaacagcacg ctaccgtcct gcaccactgt cccggcatgg acgctatcgc cttcgccttt
    30241 ttgctgcggg ataggttcgc ccgtcagcat cgcttcatcc agccatgctt cgccctgggt
    30301 aatctctcca tctaccggta cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg
    30361 cacttccgcc agtggcacgc ttttttcacc ttcgtcagta accaggcgcg ccgtcggcgg
    30421 agttaaatcg agcaatttct ccagcgcctt tgaagaacgc tgacgtgcgc gcgcttccag
    30481 catatgaccg agattaatca gaccgataat catcgcactg gcttcatagt aaagatgccg
    30541 cgcttccatc gggaaccact gcggccacag gttgacgctc atcgaataga gccacgccac
    30601 acccgttccc agcgccacca gcgtatccat cgtcgccgca ccgttcagca ggcttttcca
    30661 tgcgctgcgg taaaaatgcc cgccggcgaa aaccattacc gccagggtta tcaggccgat
    30721 aactaaccac aggctgcggt tgtcagcggt gaccatcatg ttatcgccga tcatccccca
    30781 gaccatcacc gggataccca ccgccagcgc gacaattgcc tgccagcgga agcgcttcat
    30841 cgtagcgacg gcggtttctt gctggcgctc gcggcgttta gcgtcatctt caatcgcttc
    30901 cgcgccgtag cccgcttttt ccaccgcctg cactaaatct tgtggggagg cactgcccat
    30961 caccagcgca gtacgctccg ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg
    31021 caacgcattt tgtacgcggg tgacacagct ggcgcagctc atgccgctca gcagcaactg
    31081 ctggctgtca tcgtcatcgg cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc
    31141 ttccgacggg attgatgact ccgccagcgg tttagccttt gggtggctta cagatgcgtc
    31201 ataacccgct tgtttgatgg tttcgattag ctgttctgca ctggcagtcc cggtaacgtg
    31261 cgcttcagtg atagacacat ccgcctgttc aacatccggg cgctgttcga gactttcttt
    31321 cacgcgttta acgcagtgac cgcaggacag gccgtccagg gtcaggtcga tagtttgtga
    31381 cataaaacac tcctttaaga cagttttgac tggctgtgat aaaggttaaa ccttccagca
    31441 aggggaaggt caagaaatta ataaaccagg cgggtaaaag tccgtaaaga ttaaaaaatc
    31501 ggctcgattt gcatcaggat tagacattta tctctttgtt ttcctgtagt taagttgcgg
    31561 gtgctaagtt aaatcaggat gcatgaaaat cggcaccggg gtgaggaatt acctcccgca
    31621 tctataaaaa ggagttaaca aaagatgtta gatgcaaaca aattacagca ggcagtggat
    31681 caggcttaca cccaatttca ctcacttaac ggcggacaaa atgccgatta cattcccttt
    31741 ctggcgaatg tccctggtca actggcggca gtggctatcg tgaccagcga tggcaacgtc
    31801 tatagtgcgg gtgacagtga ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg
    31861 ttagcccttg cgttagaaga tgtcggcccg caggcggtac aggacaaagt tggcgctgac
    31921 ccgaccggat tgccctttaa ctcagttatc gccttagagt tgcatggcgg caaaccgctt
    31981 tcgccactgg taaatgctgg cgctattgcc accaccagcc tgattaacgc tgaaaatact
    32041 gaacaacgct ggcagcgaat tttacatatc caacaacaac tggctggtga gcaggtagcg
    32101 ctctctgatg aagtcaacca gtcggaacaa acaaccaact tccataaccg ggccattgcc
    32161 tggctgctgt actcagccgg atatctctat tgtgatgcaa tggaagcctg tgacgtgtac
    32221 acccgtcagt gctccacgct catcaatact attgaactgg caacgcttgg cgcgacgctg
    32281 gcggcgggtg gtgtgaatcc gctgacgcat aaacgcgttc ttcaggccga caatgtgccg
    32341 tacattctgg ccgaaatgat gatggaaggg ctgtatggtc gctccggtga ctgggcgtat
    32401 cgcgttggtt taccgggcaa aagcggtgta ggtggcggta ttctggcggt cgttcctggc
    32461 gtgatgggaa ttgccgcgtt ttcaccacca ctggacgaag aaggcaacag tgttcgcggt
    32521 caaaaaatgg tggcatcggt cgctaagcaa ctcggctata acgtgtttaa gggctgatca
    32581 tgatgaacac ggaaggtaat aacggtaaca aacccctcgg tctatggaac gtcgtttcca
    32641 tcggcattgg ggcaatggtg ggggcgggga tcttcgcgct gctggggcag gctgcgttgc
    32701 taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg
    32761 gttatgccta tgcgcgtctg ggggcgagct atcccagcaa tggcggtatt atcgacttct
    32821 ttcgtcgcgg attaggcaac ggcgtttttt cgctggcgct ctcgttattg tacctgttga
    32881 cgctggcggt gagtatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt
    32941 tgcatgaagg cagtcaggag gagcacctta ttttgctcta cgcattgggg atcattgcgg
    33001 tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg
    33061 tcggcattaa aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac
    33121 cggcgcatat ttccgtttct gcgcccccca gctccggggc gttcttctcc tgtatcggga
    33181 taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag
    33241 atccggaggt cataatgcca cgggcgtttc tggtggcgat tggcgttacc acgttgcttt
    33301 atatctcgct ggcgctggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg
    33361 ccgataccgc cgtagcgcag gctgcttctc cgctcctcgg gcatgtgggg tatgtgatcg
    33421 tcgtcatcgg cgctttactg gcgacggctt cagccatcaa cgcaaacctg ttcgccgtgt
    33481 ttaacatcat ggacaacatg ggtagcgaac gcgaactgcc gaagctaatg aataaacctc
    33541 tgtggcggca gagtacctgg ggtaacatca tcgtcgtggt gttgattatg ctgatgacgg
    33601 cggcgctgaa tttaggctca ctcgccagcg ttgccagtgc cacctttttg atctgctatc
    33661 tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc
    33721 ttatcgttag tacgttggtt atgttgttgg tgatcgttgg ctttatctac agtctctggt
    33781 cccagggcag ccgtgcgctg atatggatta ttggcgcact cttactcagc cttattgtgg
    33841 caatggtcat gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acctcctgta
    33901 ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa
    33961 accctttaac aaagcacagg aggcgttgcg cgaacgatga acattagcga tgtagcaaaa
    34021 attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg
    34081 ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc
    34141 ttactgcgcc aggcacgcca ggtgggcttt aacctggaag agagcggcga actggtgaat
    34201 ctgtttaacg atccgcagcg tcacagcgcc gacgtcaaac gtcgtacgct ggagaaagta
    34261 gcggagatcg aacagcatat cgaagaactg caatccatgc gtaaccagct gctggcgctg
    34321 gcgaatgcct gccccggtga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc
    34381 tgctgtcatc atcgggcggg gtgattaagg cttgcgaaaa tagtcgtcca ttattttcgt
    34441 tagctccgga cgttccccgt catcattgat ggcaaaatcc agaaaaaaag catctgcttg
    34501 cggcgttcgg ttttgcgcca tcaaccggaa ggtgaggggg cctgaccagg tttcggatgg
    34561 gctgatgtgc ttatcagggc cgaaactctc ccatgcgctg ggcggtaatg ttttgcacgc
    34621 ggtgagtttt ggtatgtggt attgcagctc tgttttgtgc agacattccc cgccaggaaa
    34681 taccagggtt tgttgctcat cattaaaatg ggttgccgtg ctgagcacca gtgttttgag
    34741 cggtcgccct tccagcgcgg cggcagtagc cttatggtgt ccgtcgagta gaacgcagag
    34801 ataggaatca gggagatacc aggccagacc acgggcaatt ccagttttcg agagcagaca
    34861 aaaaccgggc ggctccctgt tcatcctccg caaaaccgct gtagacaaat tccgcacagg
    34921 tagggcatcc agcctcggac cagaaacgta ttaacgggac atcctgccag cagagcgaat
    34981 ggataagacc gtgcgagtgc ggatcgtaaa tcacacattg acgcagaaat cctttcccgg
    35041 acttcaccga aaaaatgacg cccgcagcgg tatttttcag cagacgtact tcaggttttc
    35101 gaaaggggga aacatggcta ctccttagcc gaacattcaa ttaacactat gtggggggct
    35161 atatgccgga tgcggcgtaa acgccttatc cggcatgggt tttggtacag atttccaggt
    35221 gtgataagac gcgtcaggcg atgaccgcac ggataatcag cgttattcct tctatcgcaa
    35281 tcacctcaac atgcgtacca gcgccgagat cctcgctggc gctgacaggc catgaactgt
    35341 caccgacgcg catatgaccg cgcccgttga ccagcggaga ttccagaaca aaacgtcggc
    35401 caatcaactg ctgcccgcgc tggtttaaat gactgtcgct gtgcttttgt tcgcgcaccc
    35461 gccgcgacaa ccatttccac cacagccagg cggcgagcag cgtcaggacg gcaaacatta
    35521 ccccttgcca ctcccaaccc agcggcagca accagaccac cagaccggta atcaccgctg
    35581 ccacgccgct ccacaacaga taaccattgc cgcccaacat ctcggctgcc agcagcaaac
    35641 cgccgagact tagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt
    35701 ccgtttgttg gcgctgtctt tcaccagctc agcaatcccg gcaatcgacc ccatcaggct
    35761 gctggcctct aatggcatca tcaccacttt gctgttactg gacgaaccga tctgctgtaa
    35821 cgcttcggtg tatttctgtg cgacgaagta gttcaccgcc tgaatatcac cggaggcgat
    35881 ggcttcagac accattttgg tggcgcgggc ttctgcttca gcggaacgtt cacgcgcttc
    35941 agcctgtaaa aacgccgact gacgttcgcc ttccgctttc aggatttgtg actgtttttc
    36001 accttcggct ttgaggattt ccgcctgacg gatcccttcc gcttcaagga tgtaagcgcg
    36061 tttggtacgt tccgctttca tctgcgcgtt cattgaagag ataagctctg cgggcgggcg
    36121 cacatcacga atttcgatac gggtgacttt aatcccccac gggttggtgg cctcatcgac
    36181 aatatgcagc agacgcgagt taatgctgtc gcgctgagag agcatttcat caagttccat
    36241 tgaacccagc acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt
    36301 gctgacttca taagccgcgc gcggcgcgtc aatcacctga ataaagcaca cagcgtcgat
    36361 ggtaacgttg gcgttatctt tcgagataac ttcctgggaa gggatgtcga gcacttgctc
    36421 catcatattg atcttgcgac caatgcgatc cataaacggc accaccaggc ttagacctgg
    36481 ctgtaacgtt ttggtatagc gaccaaagcg ttctaccgtc cattgatagc cctgcggcac
    36541 gattttgacg cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat
    36601 aagcatcgga aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa
    36661 acaaattata cctgattact gaaagagagt tcccccttat tcctgcgaag gataaactgt
    36721 ttttagtaaa aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct
    36781 acaaaacgta ggatatctgg cgggtgatac gaagattctt aataacatca atttttcgct
    36841 gcgtgctggc gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacactgct
    36901 aaaaatagtt gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga
    36961 tgtcagcaca ctaaagccag aaatctaccg tcagcaagtc tcttactgcg cccagacgcc
    37021 gacgctgttt ggcgatacgg tatacgataa cctgattttt ccctggcaga tccgtaacca
    37081 gcagcctgac ccggccattt ttctcgattt tcttgaacgc ttcgctttac cggacagcat
    37141 tttgacgaag aatatcgccg agctatctgg tggtgaaaaa caacgcatct cattgattcg
    37201 taacctgcaa tttatgccga aggttttatt gctcgacgaa atcaccagtg cgctggatga
    37261 aagtaataaa cataacgtta atgagatgat ccaccgttat gtacgcgagc aaaatattgc
    37321 tgtgctgtgg gtgacacacg ataaagacga aattaatcat gcggataaag tgattacact
    37381 gcaaccgcat gctggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg
    37441 aatcattagc actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa
    37501 aactggcgct ggagaaagat attctctgga gcgtcgggcg cgcgataatc cagctgatta
    37561 ttgtcggcta tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga
    37621 tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg
    37681 ctaaagcttt tatctcatcg tttattgcca ttacggtcgg ggcgggaatt accctggcgg
    37741 tactgatcct ctctgggtcg attgaattta tcccgatgca ggtgatccct atcgccggga
    37801 tgatagccgg taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg
    37861 tcattagcga acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaaacagg
    37921 cttcagcgat attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag
    37981 caaaaacggt aggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga
    38041 ttgatccggt gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaaccg
    38101 ccagcttgtc gaccattatt gcctgctatt taacctatcg taagttttac aattcgcgcc
    38161 accaattggt ggtgacgcaa ttgaagaaga aatgatgcga tgccggatgc gtcttatcag
    38221 acctacatgg cacctgcaat gtattgaatt agcacggttt ggtaggccgg ataaggcgtt
    38281 cacgccgcat ccggcggcta tgcgcggatg cctgatgcga cgctggcgcg tcttatcagg
    38341 cctacaaacc agcacagcat cgcatttcaa cggaaaatca atacaacaac gcatacagct
    38401 ggcggcgata cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg atctcctgga
    38461 atgttttacg tgtctgacca tctgcggcgg tgagatcttt acgcagatgc ccaaacagta
    38521 actccagcgc ctcttcattg cgcccaacct ggtgcagttg cagtgccagt tgcgtcgcca
    38581 gtgcggcatc ttctggattc tccgccacct gctgttgcaa ctgttgaatt tccggcgtat
    38641 cagccgcctg cttcagcagt tcgatttgcg ccaccagccc ctgatagcgg gtgtcctgat
    38701 cctgcaacgg aatggttttc agcacggctt ctgcatcttc agaacggttc agcgcaatca
    38761 gcgtttctgc caacagcagg ccgatttccc cgttctgatt cgacaactgc caggcgtctt
    38821 tcagcaatgg cagggcgtcg gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg
    38881 ctttcagctc ttcttcacgc ggcagcactt tatccagcag ggcgcggatc gcctcttccg
    38941 gttgcggccc ctggaagccg tctactggtt gcccgttctg gaacagatat acggtcggaa
    39001 tcgcgcgcag accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca
    39061 gaataaattg cccgttgtac tgcgccgcga ggctttccag aattggggtt aactgcaaac
    39121 agtgctggct acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa
    39181 gaacctgttg caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc
    39241 tctctgttgt cgatattttc tttgacatgg gggcttaagc gcgcgcttca actcacccct
    39301 gcaatatttt gtccatcacg cgccccggca gcaggcgctt aagcaccatt accgccctgg
    39361 tcaccagcgt caccggatag cgcatcttcg gcttctcgct aataaaagca tggcgcactt
    39421 tgccgaccac cgcttccggt cccaacgtaa agcgggcggc gatgccagga ttttcgactg
    39481 gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccaggtt
    39541 cgatcaggct gactttaatt ccgctgtggc gcagctccat gcgcagtgcg tctgaccacg
    39601 cctccagcgc atatttactg gccgcgtaag cgccacgacc cggtgtggag attaatccca
    39661 tcaccgatga ggtcatcaca atacgccctt cacggtgcgg caacatcgcg ggtaacaggc
    39721 gcatggtgag ctggtgtgcg ccgaaaaagt tagcggaaaa ctgctgttcc atctgcgcac
    39781 ggctgatggt ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca
    39841 gacaattatc ggtcagggcg atcacttcgt cggctgcgcg atcaacactt tctggcgaat
    39901 ccagatcgat caacacgccg gtaaatccca tgctgttcat gcgctcaaca tcatccggtt
    39961 tccggcaacc tgccagcaca tgaaaaccct ggcgttttaa ttcgagtgcg ctttccaggc
    40021 caataccact ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga
    40081 tctccgttgc tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg
    40141 gcaataaacg gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc
    40201 ttgaggtaga cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt
    40261 ttggggtaaa tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt
    40321 atttgcatta acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc
    40381 gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccgt cattgccgcc cagttcaacc
    40441 agcacccagc gcggctgatg ctgtttcagc agagccggaa ggcgcgccag cccttgttgc
    40501 gaggtgtcac cgctgatgct ggcattgact accgacgttt tactctgcca cttatcattc
    40561 aacaaggcag gccaggccgc gctggcagac attcgatacc cggcgctcag gctatcaccc
    40621 agaatcaata acgtgtccgc tgcggcggca cggaaggtta acaggaccag gaacaggaag
    40681 ggcaaatgcc agcggaaaac attgttgaag ttcatcatct taagaagtcc gtcggtcagg
    40741 gggagcatga actctccatc ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca
    40801 tcgcactggt gggcgagtcg ggatcgggta agtcaacctt gctggcgatc ctcgccgggc
    40861 tggatgacgg cagcagtggc gaagtgagtc tggtgggaca accgctacat aatatggacg
    40921 aagaagcgcg ggcaaagttg cgcgcgaagc acgtaggctt tgtttttcag tcatttatgt
    40981 taattcctac ccttaacgcg ctggaaaacg tcgagcttcc ggcgctgctg cgcggtgaga
    41041 gtagcgcaga aagtcgtaac ggggcgaaag cgttgctcga acagttaggg ctgggtaaac
    41101 gtcttgatca tcttccggca cagctttctg gcggcgaaca gcaacgagtg gcgctggcgc
    41161 gagcctttaa tggtcgacct gatgtgctgt ttgccgacga acccaccggt aaccttgacc
    41221 gccagacggg cgataaaatt gccgatctgc ttttttccct caaccgtgag catggcacca
    41281 cgttgattat ggtgacccac gacctgcaac tggcggcacg ctgtgaccgc tgcttacggc
    41341 tggtgaacgg gcagttgcag gaggaagcat gattgcacgt tggttctggc gcgaatggcg
    41401 ctcgccgtcg ctattaattg tctggctggc gcttagcctg gcggtggcct gcgtgctggc
    41461 gctgggcaat atcagcgatc gcatggagaa gggcttaagc cagcaaagcc gtgagtttat
    41521 ggcgggcgat cgggcgttgc gcagttcgcg cgaagtgccg caagcatggc tggaggaagc
    41581 gcaaaagcgc ggcctgaaag ttggcaagca gctgactttc gccaccatga cctttgcagg
    41641 cgacacgccg caactggcga acgtcaaagc ggtggatgat atctacccga tgtatggcga
    41701 tctgcaaact aatccccctg gcctgaaacc gcaggcgggc agcgtattgc tggccccacg
    41761 cctgatggca ctgcttaacc tgaaaacggg cgataccatt gacgtgggcg atgccacctt
    41821 gcggattgcc ggagaagtga ttcaggaacc ggattccggt tttaatcctt tccagatagc
    41881 cccacgcctg atgatgaatc tggcggatgt tgataaaacc ggagccgtgc agccggggag
    41941 tcgggtcacc tggcgttata aattcggcgg cagcgagaac cagctcgacg gctatgagaa
    42001 atggttgtta ccccagctta aacccgaaca acgctggtac ggtctggaac aggacgaagg
    42061 cgcgctgggg cgatcgatag aacgttcgca acagttcctg ctgctttcgg cgcttctgac
    42121 cttgctgctg gcggtggcgg cggtcgcggt ggcgatgaat cattactgtc gcagtcgcta
    42181 cgatctggtg gcgatcctca agacgctggg ggcagggcga gcgcaactgc gtaagttaat
    42241 cgtcggtcag tggttgatgg tgctggtact ttcagccgtt accggtgggg cgataggcct
    42301 gttgttcgaa aacgtgttga tggtgctgct caagcccgtt ctgcctgctg cactaccgcc
    42361 agccagcctc tggccgtggc tgtgggcgct cggcaccatg acggttattt cgctgctggt
    42421 ggggctacga ccataccgcc tgttgttggc aacgcagcct ttacgcgtat tacgtaatga
    42481 tgtggtagcg aacgtctggc cgctgaagtt ttatctgccg attgtcaccg tggtggttgt
    42541 gctactgctc gccgggctga tgggcggcag tatgctgctc tgggcggtgc tggcgggcgc
    42601 ggtagtactg gctttgctgt gcggtgtcct gggctggatg ctgctgaatg tacttcgccg
    42661 catgacgctg aaatcgctac ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg
    42721 ggcgacgttg agccagcttt cggcattttc gctctccttt atgctgctgg cactgttgct
    42781 ggtgttgcgt ggcgatctgc tcgaccgctg gcaacagcag ctaccgccag aaagtcctaa
    42841 ctacttttta atcaacatcg ctacggaaca ggttacgccg ctgaaagcgt ttctcgcgga
    42901 acatcagata gtcccggaat cgttttatcc ggtggtgcgg gcgcggttga cggcgattaa
    42961 cgacaagccg acagaaggca atggagatga ggcgctcaac cgcgagctga atctcacctg
    43021 gcaaaatacg cggcctgatc ataacccgat cgtcgccggt aactggccgc caaaagccgg
    43081 tgaagtgtcg atggaagagg ggctggcgaa acgcttaaac gttgccctcg gcgataccgt
    43141 gacttttatg ggcgataccc aggagttccg cgctaaagtg accagcctgc gcaaagtgga
    43201 ctgggaaagt ctgcggccta atttctattt tattttcccg gaaggggcat tagacgggca
    43261 accgcagagc tggcttacca gtttccgctg ggagaatggc aacggcatgt tgacgcaact
    43321 caaccgccag ttcccgacca ttagcctgtt agacattggc gcgattttaa aacaggtcgg
    43381 tcaggtgctg gagcaggtaa gtcgggcgct ggaagtgatg gtggtgctgg tcaccgcctg
    43441 cggtatgttg ctgttgctgg cacaggtgca ggtgggaatg cgtcagcgtc atcaggagct
    43501 ggtggtgtgg cgcactctcg gtgcgggcaa aaaactgctg cgcacgacat tgtggtgtga
    43561 gttcgccatg ctcgggtttg tatccggcct ggtggccgca attggtgcgg aaacggcact
    43621 ggcggtactg caatcgaaag tgtttgattt cccgtgggag ccagactggc gattgtggat
    43681 tgtgctgccg tgcagcggtg cgctactgct gtcgctttgc ggcggctggc tgggtgcgcg
    43741 actgcttaag ggtaaggcgc tattcaggca gtttgtgggg tgatgtgata aagccgttta
    43801 tttctacctg gtagataaac ggctttatta ttagtgcatc acgtaattaa aaaaatgtta
    43861 tgcccaaaca tataattaat ataca
//
LOCUS       DAPQZD010000019        43406 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPQZD010000019 DAPQZD010000000
VERSION     DAPQZD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.019"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            107..349
                     /locus_tag="RO454_001057"
     CDS             107..349
                     /locus_tag="RO454_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932926.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            464..1102
                     /locus_tag="RO454_001058"
     CDS             464..1102
                     /locus_tag="RO454_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDY0932927.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1099..1836
                     /locus_tag="RO454_001059"
     CDS             1099..1836
                     /locus_tag="RO454_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDY0932928.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1836..3932
                     /locus_tag="RO454_001060"
     CDS             1836..3932
                     /locus_tag="RO454_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDY0932929.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3979..4257)
                     /locus_tag="RO454_001061"
     CDS             complement(3979..4257)
                     /locus_tag="RO454_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0932930.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4472..4882
                     /gene="psiE"
                     /locus_tag="RO454_001062"
     CDS             4472..4882
                     /gene="psiE"
                     /locus_tag="RO454_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDY0932931.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4976..5866)
                     /gene="malG"
                     /locus_tag="RO454_001063"
     CDS             complement(4976..5866)
                     /gene="malG"
                     /locus_tag="RO454_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDY0932932.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5881..7425)
                     /gene="malF"
                     /locus_tag="RO454_001064"
     CDS             complement(5881..7425)
                     /gene="malF"
                     /locus_tag="RO454_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDY0932933.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7579..8769)
                     /gene="malE"
                     /locus_tag="RO454_001065"
     CDS             complement(7579..8769)
                     /gene="malE"
                     /locus_tag="RO454_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDY0932934.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9134..10249
                     /gene="malK"
                     /locus_tag="RO454_001066"
     CDS             9134..10249
                     /gene="malK"
                     /locus_tag="RO454_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDY0932935.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10321..11661
                     /gene="lamB"
                     /locus_tag="RO454_001067"
     CDS             10321..11661
                     /gene="lamB"
                     /locus_tag="RO454_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDY0932936.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11894..12814
                     /gene="malM"
                     /locus_tag="RO454_001068"
     CDS             11894..12814
                     /gene="malM"
                     /locus_tag="RO454_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDY0932937.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            12993..13490
                     /gene="ubiC"
                     /locus_tag="RO454_001069"
     CDS             12993..13490
                     /gene="ubiC"
                     /locus_tag="RO454_001069"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDY0932938.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13503..14375
                     /gene="ubiA"
                     /locus_tag="RO454_001070"
     CDS             13503..14375
                     /gene="ubiA"
                     /locus_tag="RO454_001070"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDY0932939.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14530..16953)
                     /gene="plsB"
                     /locus_tag="RO454_001071"
     CDS             complement(14530..16953)
                     /gene="plsB"
                     /locus_tag="RO454_001071"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDY0932940.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTKQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17124..17492
                     /gene="dgkA"
                     /locus_tag="RO454_001072"
     CDS             17124..17492
                     /gene="dgkA"
                     /locus_tag="RO454_001072"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDY0932941.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17602..18210
                     /gene="lexA"
                     /locus_tag="RO454_001073"
     CDS             17602..18210
                     /gene="lexA"
                     /locus_tag="RO454_001073"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDY0932942.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18283..19608
                     /gene="dinF"
                     /locus_tag="RO454_001074"
     CDS             18283..19608
                     /gene="dinF"
                     /locus_tag="RO454_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDY0932943.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            19724..19933
                     /locus_tag="RO454_001075"
     CDS             19724..19933
                     /locus_tag="RO454_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDY0932944.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(19975..20490)
                     /gene="zur"
                     /locus_tag="RO454_001076"
     CDS             complement(19975..20490)
                     /gene="zur"
                     /locus_tag="RO454_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDY0932945.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20646..21641
                     /gene="dusA"
                     /locus_tag="RO454_001077"
     CDS             20646..21641
                     /gene="dusA"
                     /locus_tag="RO454_001077"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDY0932946.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21775..22017
                     /gene="pspG"
                     /locus_tag="RO454_001078"
     CDS             21775..22017
                     /gene="pspG"
                     /locus_tag="RO454_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDY0932947.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(22182..23165)
                     /locus_tag="RO454_001079"
     CDS             complement(22182..23165)
                     /locus_tag="RO454_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDY0932948.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(23271..25307)
                     /locus_tag="RO454_001080"
     CDS             complement(23271..25307)
                     /locus_tag="RO454_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDY0932949.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            complement(25318..26598)
                     /locus_tag="RO454_001081"
     CDS             complement(25318..26598)
                     /locus_tag="RO454_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDY0932950.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            complement(26625..27404)
                     /locus_tag="RO454_001082"
     CDS             complement(26625..27404)
                     /locus_tag="RO454_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDY0932951.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            complement(27397..28968)
                     /locus_tag="RO454_001083"
     CDS             complement(27397..28968)
                     /locus_tag="RO454_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDY0932952.1"
                     /translation="MCSVRTITAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERF
                     QRAGHPQNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQM
                     INDNAIPAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSL
                     VERVRFANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGG
                     IIIAQVKRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSA
                     FRYMEHGPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAI
                     GGVPLLEFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPAR
                     PHVTAGCGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVT
                     FSGKRAIRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDL
                     KLMNPLYFQQGSHHE"
     gene            29224..30258
                     /locus_tag="RO454_001084"
     CDS             29224..30258
                     /locus_tag="RO454_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDY0932953.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            30331..31746
                     /gene="dnaB"
                     /locus_tag="RO454_001085"
     CDS             30331..31746
                     /gene="dnaB"
                     /locus_tag="RO454_001085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDY0932954.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            31799..32878
                     /gene="alr"
                     /locus_tag="RO454_001086"
     CDS             31799..32878
                     /gene="alr"
                     /locus_tag="RO454_001086"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDY0932955.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            32901..33458
                     /locus_tag="RO454_001087"
     CDS             32901..33458
                     /locus_tag="RO454_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDY0932956.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            33455..34459
                     /locus_tag="RO454_001088"
     CDS             33455..34459
                     /locus_tag="RO454_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0932957.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            34541..35734
                     /gene="tyrB"
                     /locus_tag="RO454_001089"
     CDS             34541..35734
                     /gene="tyrB"
                     /locus_tag="RO454_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDY0932958.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            35916..38660
                     /locus_tag="RO454_001090"
     CDS             35916..38660
                     /locus_tag="RO454_001090"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDY0932959.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            38693..39847
                     /gene="odhB"
                     /locus_tag="RO454_001091"
     CDS             38693..39847
                     /gene="odhB"
                     /locus_tag="RO454_001091"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDY0932960.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            39859..41276
                     /gene="lpdA"
                     /locus_tag="RO454_001092"
                     /pseudo
     CDS             39859..41276
                     /gene="lpdA"
                     /locus_tag="RO454_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
     gene            41298..42467
                     /gene="sucC"
                     /locus_tag="RO454_001093"
     CDS             41298..42467
                     /gene="sucC"
                     /locus_tag="RO454_001093"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDY0932961.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            42480..43352
                     /gene="sucD"
                     /locus_tag="RO454_001094"
     CDS             42480..43352
                     /gene="sucD"
                     /locus_tag="RO454_001094"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDY0932962.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
BASE COUNT        10295 a        10918 c        11748 g        10445 t
ORIGIN      
        1 atcactaatt ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg
       61 aaatgttttg ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct
      121 gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca
      181 ggtgggcgaa gcggcaggat cggcagcaac gtcggtttcg gcgggtagtt cctccgcgac
      241 cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga
      301 tggttctaat accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta
      361 tccccaaaat aattcgagtc atcgcatctg aagcttgaag tatgaagggg attaaccata
      421 accacactcc ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gaccagcact
      481 cattcttatc tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc
      541 actgtgggac agtctgttcg gtacgccagg cgtacagctg acggacgatg atattcaaaa
      601 tatgccctac gccagccagt acatgcagct taatggcggg ccgcagttat ttgtggtgct
      661 ggccttcgct gaagacggac aacaaaaatg ggtcactcag gatcaggcca cccttgtcac
      721 acaacatggt cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa
      781 ccttgccgcc gacccgctga ttaaacccgc acaaattgtt gatggtgcaa cctggacgcg
      841 cacgatgggc tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa
      901 atgggatggc accgataccg tcaaagtcgg cagcgatgaa accccggttc gcgtgctgga
      961 cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg
     1021 gcaaattcgc cagtcggaac agtatctcgg cgcggattac ttcccggtga aaaccaccct
     1081 catcaaggcg gcaaaacaat gattaaacaa actattgtcg cattgatttt gagtgtgggg
     1141 gcgtcatcgg tcttcgcggc gggaaccgtc aaggtattca gcaatggcag cagtgatgcc
     1201 aaaacgctga cgggtgcaga gcatttaatc gatctggtag gccaaccgcg gctggcaaac
     1261 agctggtggc ctggtgcggt gattagcgaa gagctggcca cggcggcagc attgcgtcag
     1321 cagcaggcgt tgatgacacg gatggcagaa ctggccgcag actccagcgc cgacgatgcc
     1381 gccgcaatta acgccttacg ccagcaaatt caggcgttga aagtgacggg caggcaaaaa
     1441 atcaatcttg atcctgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc
     1501 aactacacgc tgtgggtcgg accgccgccg tccacggtca cgttgttcgg gcttatcagt
     1561 cgtcctggca agcagtcatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag
     1621 agcctgctca gcggtgcgga tcgcagctac gcgtgggtgg tttacccgga cggacgcacg
     1681 caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt
     1741 atttatgttg gcctcgcgga ctccgtctgg agtgagacac ctgatgccct taacgccgac
     1801 attcttcaga ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct
     1861 tactggcgct gggtattagc acagcttgct acggcgaaac atatcctgcg cccattggtc
     1921 cgtcgcaatc ggatttcggt ggcgtaggat tattacaaac gcccaccgcg cgcatggcgc
     1981 gggaagggga gctgagcctg aactatcgcg ataacgatca gtaccgttat tactcagctt
     2041 cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc
     2101 agtacagcag cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc
     2161 tcaaactgcg tttgtgggaa gagagttatt ggctgccgca agtggcggtt ggtgcgcggg
     2221 atatcggcgg tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc
     2281 cgttcgattt tacactcggc ctgggctggg gatatctggg caccagcggt aacgtgaaaa
     2341 atccgctctg ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg
     2401 gatctatcga cggcagccag atgttccacg gcccggcctc actgtttggc ggcgtggaat
     2461 accagacgcc ctggcaaccg ctgcgtctga aactggagta tgaaggcaat aattatcagc
     2521 aggattttgc cggaaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg
     2581 ttaccgattg ggccgacgtt aaccttagct atgaacgtgg caacactttt atgtttggcg
     2641 tcacgctgcg caccaacttt aacgatctgc gcccgtctta caacgataac gcccgcccgc
     2701 aatatcaacc gcagccgcag gatgccattt tgcagcattc ggtggtggcg aatcagttaa
     2761 cgctgttgaa atacaatgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc
     2821 tgtatgttac cggcgagcag gtgaaatatc gtgatccgcg cgaagggatc atccgtgcta
     2881 atcggatcgt gatgaacgat ctgccggatg ggataaaaac gatccgcatt acggaaaatc
     2941 gccttaatat gccacaggtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg
     3001 ccggagagcc gttgggccac gaaacgaagc tggcgcaaaa acgcgtcgag ccagtggttc
     3061 cgaagtccac cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc
     3121 cggtgctgaa ccagtcgatc ggtggcccgg aaaactttta catgtatcag ctgggcgtga
     3181 tgggaacggc ggatttgtgg ctgacggacc atctgctgac cactggcagc ctgtttgcta
     3241 accttgccaa caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac
     3301 cgcgcgtgcg tacccatgtg cgcgagtatg tgcagaatga tgtctatgtg aataacctgc
     3361 aagccaacta cttccagcat ctgggcaacg gcttctacgg tcaggtctac ggaggttatc
     3421 tcgaaaccat gtttggcggc gtgggggcag aagtgttgta tcgcccgctg gatagcaact
     3481 gggcgtttgg tctggatgcc aactacgtta aacagcgcga ctggcgtagc gcaaaagata
     3541 tgatgaaatt caccgattac agcgtgaaaa ccggacatct gaccgcgtac tggacgccat
     3601 ctttcgccca ggacgtgtta gtgaaagcga gcgtcgggca gtatctggct ggggataaag
     3661 gcggcacgct ggagatcgct aaacgctttg acagcggcgt ggtggttggc ggctatgcca
     3721 cgatcacgaa tgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg
     3781 tctctgtacc gctggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct
     3841 ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca
     3901 tgaccagcga ccgtagcgtc aatttccgct aagtcatggg aaaggtgcca ggtttcgcac
     3961 tcactggcac ctttattctt aatggcctgt cgagccatca ggaacgcagt tcacccccgg
     4021 tttgcgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg
     4081 caggtctggt tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag
     4141 gtcggcttta tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa
     4201 catcaaagca gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg
     4261 ttccagagga acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg
     4321 gtgatttttt tgggctgtgt gttttgccag atgagacatt gatgggagat gcccacttaa
     4381 ttgagctgaa caaaacatac acaaaaaata tagatctccg tcacattttt gcgttataca
     4441 ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga
     4501 gtttatctcc accattttgc agaccgtact caatcttggc ctgttgtgcc tcggcctgat
     4561 tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt tcgcgccaga
     4621 acaaaccagt aaatatgagc tggtagaagg tctggtggtt tactttctct atttcgaatt
     4681 tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac gttactttgt
     4741 ctatattggg atcactgcca ttgtgcgctt gatcatcgtc gatcataaat cgccactgga
     4801 tgtgttgatc tactcggcag cgatcctgtt actggtgatc accctgtggt tgtgtaattc
     4861 gaaacggctg aagcgggagt aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa
     4921 gttgggataa cgtaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
     4981 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
     5041 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
     5101 gcgggttgag gtattgctgc atccccacgg ccagggtgta gctgtttacg tcacgcagta
     5161 atagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaggatg aacacaaccg
     5221 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
     5281 catccagcgc cgccgcttct tccagcgaac tgtcgatggt ttcgaaataa cctttgatgg
     5341 tccagacatg cagcgcaatc ccgcccagat acgcgaaaat tacgccgccg tgagtattca
     5401 ggccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
     5461 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
     5521 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg
     5581 cggaaatccc ggcgaccttt accgagttcc acagccacag cagtaccggg aatggcggtg
     5641 gcgtaatgcg accatcagcc tgttccacgc taaaacctaa cgccagtttc cagtgatccc
     5701 aggagatttg ctccgggatc agactaccgg tcgcgaagtt cccctgacgc agcgagatgg
     5761 cgacgaccat cagcagcggg aacataattg ccgcgataaa aagtagcagt agcaggtgag
     5821 taataaacaa acgtgctttt tgcgatttcg gttggaccat tgccattttt gttatctccc
     5881 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcgc ccaccagcag
     5941 gaagatcagc gtggcaattg ctgctgccag accgaagtcc tgacccccgc cgccttcaaa
     6001 agcgatacgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
     6061 aagacgatcc gggccgccgt tggttaacag ttgaatcagc acgaagttgt taaagttaaa
     6121 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
     6181 aaagaagttc tggaatggac ctgcgccatc cattgctgag gcttcataca aatcgtccgg
     6241 aatcgctttc agcaagccca tgcagaggat catcatgtac ggataaccca gccaggtgtt
     6301 gacgataatc agcatcgtgc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
     6361 aaacagcgcg ctcaacatca tgttgatttc accaaaactc tggttaaaca accctttgaa
     6421 aatcaagatt gaaatgaacg atggcaccgc gtagggcaga atcagcagga cgcgatagac
     6481 cgctttgccg cgcaacgctt cccactgcac cagacacgcc agaaccatgc cgaccgccac
     6541 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
     6601 tttctgaatg ccttcgtcgg taaagacgcg ggtaaagttt ttccagccgg tggtcacggt
     6661 gtaaccgggg cttagctttt catcacccca gttaccgtcg gcagtgatgg actggtaaaa
     6721 accaatctgg tcgtttggac gatattttac gccactctga ttattggtca gcgtgccgtc
     6781 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
     6841 tttgttgcca tccggcagaa tggcggtaat gtcactcagt gcctgacgat tctgggtaat
     6901 tacgcgcaga ttcgcgcgtt cgccttcggg ctgggcggtc gtttctttca gttgcagttt
     6961 ttgctcgccg ccaaatttaa aagcgtcgga gaggtaattt ttgccggttt cgccgtcgct
     7021 gagcgccagt tgccactcat cgcccgccgg ataaagacca aaattataga ttttgcctgc
     7081 ttgccaggag cgatctagca acacttcctg cgcacgttca aaagtcagct ggttagtgct
     7141 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
     7201 cattccagcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
     7261 atacagcccc gctgagctca atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc
     7321 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctaaca ctgaccattt
     7381 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
     7441 tccaggacgg ataaggcttt cataccttat ccgacaacaa ctgcctgatg cgacgctgac
     7501 gcgtcttatc aggcctacat acgtttcaat ttcgtaggcc ggataaggcg ttcacgccgc
     7561 atccggcatt tcacagcatt acttggtgat acgagtctgt gcatctttca gcgcagcatc
     7621 cacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
     7681 ggacatctgc gggatgttcg gcataatttc acctttctga gcgttatcca tggtggcggc
     7741 aatacgtgga tctttcgcaa gttcatcctg ataggacttc agcgctactg cacccagcgg
     7801 tttgtcttta ttgaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
     7861 tttcgccagc tctttgttcg gactggcggc gttaatacct gcgctcagca cgccaacgaa
     7921 cggtttagat ggttgacctt tgaaggtcgg cagtaccgtt acaccataat tcactttact
     7981 ggtgtcgatg ttggaccatg cccacgggcc gttgatagtc atcgctgttt cgcctttatt
     8041 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaatcag
     8101 atcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccta cgtctttaat
     8161 gtcgtacttg ccgttttcgt acttgaacgc ataacccccg tcagccgcaa ttagcggcca
     8221 ggtgaagtat ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
     8281 tttatccagc gccgggatct cttcccaggt ttttggcggg ttcggcagca gatctttgtt
     8341 ataaatcagt gataacgctt cgacagcgat cgggtaagca atcaacttgc cgttgtaacg
     8401 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
     8461 agccaacagg ccagattgag catagccacc aaagcggtcg tgcgcccaga agataatgtc
     8521 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
     8581 aacggtgact ttaattccgg tatctgtctc gaatttctta ccgacttcag cgagaccgtt
     8641 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
     8701 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
     8761 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
     8821 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc atcgttgctt
     8881 tgtgtgatct ctgttacaga attggcggta atgtggagat gcgcacataa aatcgccacg
     8941 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagccg
     9001 cgttctcatc ctcccgcctc ctcccccata aaaaagccag ggggtggagg atttaagcca
     9061 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
     9121 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
     9181 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
     9241 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
     9301 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac actccgcctg cagaacgcgg
     9361 cgttggtatg gtgtttcagt cttacgcgct ctatccccac ctgtcagtag cagaaaacat
     9421 gtcatttggc ctgaaactgg ctggcgcgaa aaaagaagtg attaaccagc gagttaacca
     9481 ggttgcggaa gtgctgcaac tggcgcattt gctggatcgc aaaccgaaag cgctctccgg
     9541 tggtcagcgt cagcgtgtgg cgattggccg tacgctggtg gccgagccaa gcgtattttt
     9601 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
     9661 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
     9721 agcgatgacg ctggccgaca aaatcgtggt gctggacgct ggtcgcgtgg cgcaggttgg
     9781 gaaaccgctg gagctgtatc actatccggc agaccgtttt gtcgccggat ttatcggttc
     9841 gccaaagatg aacttcctgc cagtaaaagt gaccgccacc gcaatcgatc aagtgcaggt
     9901 ggagctgccg atgccgaatc gtcagcaagt ctggctaccg gtcgaaagcc gtgatgtcca
     9961 ggtgggagcc aatatgtcgc tgggtattcg cccggagcat ctactgccga gtgatatcgc
    10021 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacgcaaat
    10081 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    10141 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    10201 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    10261 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    10321 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    10381 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    10441 agcggcggcg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    10501 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    10561 aagagcttct atttcgacac taacgtggcc tattccgtcg cgcaacagaa tgactgggaa
    10621 gctaccgatc cggctttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
    10681 ccaggctcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    10741 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    10801 ttcggtaaac tctctctggc agcaacccgc tcctctgaag cggggggttc ttcctctttc
    10861 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
    10921 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    10981 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
    11041 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    11101 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat
    11161 aacgaaaaat ttgcctacaa tatcaataac aacggtcaca tgctgcgtat cctcgaccac
    11221 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    11281 aactgggata acgacaacgg caccaagtgg tggaccgtcg gtattcgccc gatgtacaag
    11341 tggacgccaa tcatgagcac cgtgatggaa atcggctacg acaacgtcga atcccagcgc
    11401 accggcgaca agaacaatca gtacaaaatt accctcgcac aacaatggca ggctggcgac
    11461 agcatctggt cacgcccggc tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa
    11521 tggggttatg actacaccgg tagctcttca accaacccat actacggcaa ggctgtgtcg
    11581 gctgatttca atggcggtag cttcggtcgt ggcgacagcg acgagtggac cttcggtgct
    11641 cagatggaaa tctggtggta atagcaaaaa actgggccgg ataaggtgtt tgcaccgcat
    11701 ccggcaatca atgcctgatg cgacgctgtt gcgtcttatc aggcctacaa ccgctgccga
    11761 aagtaggccg gataaggcgt ttacgccgca tccggcataa aaacagcttt aaggggcgaa
    11821 agcccctctg attatcgggt ttagcgcgct attgcctggc taccgctgag ctccagattt
    11881 tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgct ctctgtttat cagcagggtt
    11941 gctggcaagc gcacctggaa tcagccttgc cgatgttaac tacgtaccgc aaaacaccag
    12001 cgacgcgcca gccattccat ctgccgcgct gcaacaactc acctggacac cggtcgatca
    12061 atctaaaacc cagacgactc aactgtcgac cggcggccaa caactgaacg tttccggcat
    12121 cagtggtccg gttgctgcgt acagcgtccc ggcaaacatt ggtgaactga cactgacgct
    12181 gaccagcgaa gtgaacaaac aaaccagcgt atttgcgccg aacgtgctga ttcttgatca
    12241 gaacatgacc ccatcagcct tcttccccag cagttatttc acctaccagg aacccggcgt
    12301 gatgagtgca gatcgtctgg aaggcgttat gcgcctgaca ccggcactgg ggcaacagaa
    12361 actctatgta ctggtcttca ccacggaaaa agatctccag cagacaaccc aactgctcga
    12421 cccggcaaaa gcctatgcca aaggcgtagg taactcggtg ccggatattc ctgacccgat
    12481 tgcccgtcac accactgatg gtttgctgaa actgaaagtg aaaacgaact ccagctccag
    12541 cgtgttggta ggaccactat ttggttcttc cgctccagct ccggttacgg taggtaacac
    12601 ggcggcacca gctgtggctg cacccgctcc ggcaccggtg aagaaaagcg agccgatgct
    12661 caacgacacg gaaagttatt ttaataccgc gatcaaaaac gctgtcgcga aaggtgatgt
    12721 tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg gggtcgacat ctgcccgttc
    12781 cacctttatc agcagtgtaa aaggcaaggg gtaattacgc tccacagtgc tgattttgca
    12841 acaactggtg cgtctcctgg cgcacctttt tttctcctcc tgtactattg ttgcgttttt
    12901 gttgcgcatt agttcactta atttgcttta catctcccgt aaatattttt ctgcgataca
    12961 atgcctttac gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta acgcaactgc
    13021 gtgcgctgcg ctattttaca gagatccctg ccctggagcc gcaactgctc gactggctgt
    13081 tgctggaaga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg agcgtgacga
    13141 tgatccgcga agggtttgtc gagcagaatg aaatccccga agaactgcca ctgctgccga
    13201 aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa ccgtggcttg
    13261 ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc ggagctggcg ttacaaaaat
    13321 taggtaaaac gccgttagga cgctatctgt tcacatcatc gacattaacc cgggacttta
    13381 ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga ttaagcggta
    13441 aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa gaggaaaaaa
    13501 atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta atgcgtacgg
    13561 ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg tgggtggcga
    13621 caccgggcgt tccccagctc tggatcctgg cggtgtttgt cgcgggcgtc tggctgatgc
    13681 gcgctgctgg ttgcgtggtg aatgattatg ctgaccgcaa gtttgatggt catgttaagc
    13741 gcacggcgaa ccgaccactt cccagcggcg cggtgacaga gaaagaagcg cgtacgctgt
    13801 ttgtagtgct ggtactgatt tcatttttac tggtgctgac gctgaatacg atgactattc
    13861 tgttgtcaat tgccgcgctg gcgctggcgt gggtataccc gtttatgaag cggtataccc
    13921 atctaccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg gcttttgccg
    13981 ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat attctctggg
    14041 cggtggctta cgacacgcag tatgcgatgg ttgaccgcga tgatgatgtg aagattggca
    14101 ttaaatccac cgcaatcctg ttcggccaat acgataaatt gattattggt attttgcaga
    14161 ttggcgtact agcgctgatg gcgatcatcg gtgagttaaa tggcttaggc tggggatatt
    14221 actggtcaat tctggtagct ggcgcgctgt ttgtttatca acaaaaactg attgccaacc
    14281 gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt ctggtactgt
    14341 ttttagggct ggcaatgagt tactggcatt tctgatgatg taaaaaagcc ggatgatcat
    14401 ccggctttct tctgggttgc ctgatgcgct gcgcttctca ggcctacaca acatatcgca
    14461 atttattgaa tttgcagatc atggtaggcc gaataaggcg tttgcgtcgc atccggcaat
    14521 tctcactgat tacccttcgc cctgcgtcgc actctcaatc gtcaaacgca cgtctgatgt
    14581 aatcaactcc gccagcaact gatatacctt catcgtttct gccggttcgg cgtcgccgct
    14641 atcgctgata tacccttcat cacgcagcgt cagcaccaga gaactgaaca ccgccttgtc
    14701 gaagaattcc ggcgcgttga taccgtgcag cacggagaga cgttgcgcga cggtgcggct
    14761 ctctttttcc agcgtaccgc ggttgatcga cgggttggca ctcaacagcc agaaggtgat
    14821 ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtagcg tgcgagaatg
    14881 cgccgggttg atatgcaact catcatcttg cagggtaatc agcccctgac gttgcatctc
    14941 atttgccagc gcatcaataa cgtccggtaa ctcgtcgcga tcccagcgca ggaacagttc
    15001 cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc gggagatgtg
    15061 gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat gcgcaatgtt
    15121 gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc agaatgatga tgtcgccgat
    15181 agtgtctttc tcgacttcaa acttgttcat ttgcagcgcg tgatcgataa gctcgctggc
    15241 gctggctgaa ggaacggtag agtccgtgga gtaaggcacg ttgcgcatca gatccaggta
    15301 gcagttgagt tgcttggtta actgctcgcg ggtgagtgag cgctgacgtg acgccagaag
    15361 cgcagtacag cacaggttca tggcgttcgc cgcgcctgcg ttgttaatgc gtaccatcag
    15421 atcggcagca atattattga ccgtcggcgt taaccaggcc ggacgcaccg cttcgatggg
    15481 atcgatagat tcacgccagt ctggtacgtg ctggttaagg taggtcatca acggcattgg
    15541 ttcaccgaag ttgacgtaac cctgaccgag attacgcagc ttgcttaaac cgcgcagcat
    15601 ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt tctttggcgt aagtacccac
    15661 ttccatgacg tgctcataac cgatatagat cggaatcagc gtaatcggac gagtgccgcc
    15721 acgcagcatc gcctgaatgg tcatcgatag cgtaccggtt ttcggatcca gcaaacgccc
    15781 cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa taaccacggc tgaacagttc
    15841 gccgagatac tcacggaaaa cggtggaata aagtttattg cctttaaacg tacggcgaat
    15901 aaagaacgcc cccagacggc ggaaaatcgg cccggccggc cagaagttca ggttgatccc
    15961 ggcggcgata tgcggcggca ccagcccctg gtgatacagc acgtaagaaa gcagcaggta
    16021 gtccatgtga ctgcggtggc aaggcacata cactagctca tggccgtcgt gggccagctg
    16081 gcgaacgcgc tcggcgttat ggacgttgat accctggtaa agtcggttcc aggtgaagcc
    16141 cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa ttcgccgcaa tctcttccat
    16201 cagcgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc gtgcttcatc
    16261 ttctaccgct ttcgcaatgg cgcgggaggc gagcagctta ttaaacagat cctgacgagc
    16321 aggcagacgt ggacccacgg cagccagacg ttgacgggca aagtgcatac gcgccacgcg
    16381 cgccagtttc tgagcgatag ttttatccgt gccgtgttca tccgccatac ggcgcagtga
    16441 aactgacggc gagaaacgca caaaactgtc gcgaccgagc cacagtacag cgaaaaattt
    16501 ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg cctttttcac gccccggcgc
    16561 gcgaccaaac atcaccgata ctggcaccat ctgcacatcc agatttgggt tgctgcggtg
    16621 caaatcgaga tagtcgtgga acagcttaat agactcttct ttcggcgtgt aataggtgaa
    16681 cacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt cgatttccag
    16741 cggttctaac gggtcaggca ggtcatgtgc cagacactgg gcgcgcaacg tcagcaagtc
    16801 agctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca gccccagttc
    16861 cggggcagga tccgccggaa tagacttgct ttttaccagg atgcttaatg gtaaattcag
    16921 taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc tggttaataa
    16981 tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat ccgctcatgt
    17041 ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga tattgacgct
    17101 cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcatc cgaattatta
    17161 aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa gcggcattcc
    17221 gtcaggaagg cgtagcggta ttgttagcgg tggttatcgc ctgctggctg gatgtggacg
    17281 cgattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg gaaattctca
    17341 atagcgccat cgaagctgtg gttgaccgaa ttggctctga ataccatgag ctttccggac
    17401 gcgctaaaga tatgggatct gccgcagtgc tgattgccat tatcgtcgcc gtgattacct
    17461 ggtgcatcct gttatggtcg cattttggat aacccttcca gatttcgata aatctctggt
    17521 ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt atatactcac agcataactg
    17581 tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat
    17641 ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag
    17701 cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa
    17761 ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtttattgca ggaagaggaa
    17821 gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgcttctggc gcaacagcat
    17881 attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga tttcctgctg
    17941 cgtgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgattt gctggcagtg
    18001 cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga tgacgaagtt
    18061 accgttaaac gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga aaacagcgag
    18121 tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaggg gctggcggtt
    18181 ggggttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat gccgcctggc
    18241 gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt cctcacttca
    18301 tctgataaag cactctggca tctcgcctta cccatgattt tctccaatat caccgttccg
    18361 ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg atagtccggt ttatttaggc
    18421 ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta tgttgttgct gtttttacgc
    18481 atgagcacca ccgggctgac tgcacaggct tatggtgcca aaaatcctca ggcattagcc
    18541 cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggccg gggcgttaat tgcgctgctg
    18601 cgtacgccga ttatcgatct ggcgctgcat attgttggtg gtagtgaagc agtcctggaa
    18661 caggcgcgac gctttcttga aatccgctgg ttaagcgcac cggcgtcgct ggcgaatctg
    18721 gtattacttg gctggttact cggcgtgcaa tatgcccgtg cgccagtaat tttgttagtg
    18781 gtcggcaata tcctcaacat tgtgctggat gtctggctgg tgatggggct gcatatgaac
    18841 gtgcagggcg cggctctggc gacggttatt gcggaatatg caacattgct gattggtctg
    18901 ctaatggtgc gtaaaattct caaactacgc ggaatctccg gcgaaatgct gaaaacagcc
    18961 tggcgaggaa acttccgtca cttgctggcg cttaaccgcg atatcatgct gcgctcgctg
    19021 ttgctgcaac tctgtttcgg cgcgatcact gtacttggcg cgcgactggg gagtgacatt
    19081 atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc gctggatggt
    19141 tttgcctacg cggttgaagc acattccggt caggcatacg gtgcgcgcga cggtagtcaa
    19201 ttgctggatg tctggcgggc agcgtgccgc cagtcgggga tcgtagcgtt actgttttcg
    19261 gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt aacccagatt
    19321 cagcagctgg ctgaccgcta tcttatctgg caggtgattt tgccgttggt cggcgtctgg
    19381 tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat gcgtaacagt
    19441 atggcggtgg ccgccgcagg ttttgcactg acgctcctta cgctgccgtg gctggggaat
    19501 catggtttgt ggctggcgtt aaccgtcttt ctggcgttac gcgggctttc tctggcggct
    19561 atctggcggc gtcactggcg taacgatacc tggtttgtcg caacgtgacg gttaaaaatt
    19621 ctgaataaat aatcctaagc caaattgctg actacactta atctcacgtt cagaagaaaa
    19681 gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata aagatgaagc
    19741 cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca aactgaccga
    19801 tgatgatatg acgatcattg aaggaaaacg tgatcagtta gtgggtaaaa tccaggaacg
    19861 ctacggttat cagaaagatc aggcagaaaa agaggttgat agttgggaaa aacgccatga
    19921 ttaccgctgg taattaatcc ctcctgcccg acatgtacaa ggatgtacac ccttttagcg
    19981 aggtttcttt ttcacctgaa tagagtggtc atgatggcac tgttcaggat gacgacacgc
    20041 ttctacttct acacatgctg aacataatcc atgtgcttca ataacattat gccgcagggc
    20101 aaaacccatt tttgccgcca gcgtatgcat gatgtcttcc acgccttcag cacattcttc
    20161 tttcaccgcg ccgcagcgat cgcaaataaa catggctgaa gtatgggtgg gctgatcgaa
    20221 cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac cttgttcaag
    20281 cagaaactcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag cttcgcgcag
    20341 caaatcaagc agatcataag cgctgatagc gccatcctgc agactcatca ggcgcaacac
    20401 ttcgagacgc tgtggggtca ggcgcacatt acgctgtgcg cagatttttt cagcctgcgc
    20461 taataactcc tgcgttgtgg tcttttccat tgggcacctc gacagtgggg ttaagaatgt
    20521 ccttacttta ccatagttct cacttaaacg ccgagaacag ggtgtcatgg ctgtcacatt
    20581 gcctgatcgc atattcgtta tgctatagta gcgcccccaa tttaccagat gcttaaaaag
    20641 tcgccatgcc agcagaatca cacactgttt atcccgctta ccgtttctcg attgccccga
    20701 tgcttgactg gacggacaga cattgccgtt atttcctgcg tctgctttcc cgcaatacgt
    20761 tgctgtatac cgaaatggtg accacagggg cgattattca cggtaaaggt gattacctgg
    20821 cgtacagtga agaagaacat ccggtagcgt tgcaactcgg gggtagcgat ccggcggcgc
    20881 tggcacagtg tgcgaagctg gcagaagcgc gcggatatga tgagatcaac ctgaatgtcg
    20941 gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg ggtaatgcgc
    21001 agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattccg gtgacggtga
    21061 aaacgcgtat tggcatcgat gatcaggaca gctatgaatt tctctgcgat ttcatcaaca
    21121 ccgtttccgg caaaggtgag tgtgagatgt tcatcatcca cgcacgtaaa gcctggcttt
    21181 ctgggttaag tccgaaagaa aaccgtgaga tcccgccgct cgattatccg cgtgtgtatc
    21241 aactgaagcg tgactttccg catctgacga tgtcgattaa cggtggtatc aagtcgctgg
    21301 aagaagctaa agcgcatttg caacatatgg atggcgtgat ggtcgggcgc gaggcgtatc
    21361 agaatccggg gattctggcg gcggtagacc gagagatttt tggttcctcg gataccgatg
    21421 ccgatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa ctcagccagg
    21481 ggacgtatct cggccatatt acccggcata tgctgggctt gttccagggt attcctggcg
    21541 cgcggcagtg gcgacgttat ttaagtgaaa atgcccataa agcgggtgca gacattaatg
    21601 tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc aaaaagtagt
    21661 caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc aatagattat
    21721 ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag catcatgctg
    21781 gaactacttt ttgtaattgg cttttttgtc atgctgatgg tcactggtgt ttcgttgctg
    21841 ggtattatcg ccgcgctgat tgtggcgacg gcctttatgt tcctcggcgg tatgctggca
    21901 ttgatgatta agttgctgcc gtggctgctg ttggcggttg cggtggtgtg ggtgatcagg
    21961 gcgattaaag caccaaaagt gccgaaatat cagcattatg accgctggcg ttactaaggg
    22021 attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga attgataatc
    22081 aaatctgtca ctattgcgtg tcaaacaaat tcatcgagct gtaccctaca tacagccgaa
    22141 ctataaaaga aagggcttcc caggtggaag cccaatttct tttatggaat caacaggctg
    22201 gaaccctgcg tcgcccggct ttccagaatc tcatgcgcac gccgcgcatc cttcagcgga
    22261 tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt
    22321 tcattactgg cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt
    22381 gtcacataca acgagccttt ctgattgaga atgcctaagt tcacaccggt aaccgcacct
    22441 gatgagttgc caaaactcac cattaagccg cggcgttgca ggcaatccag cgaccgttcc
    22501 caggtgtctc tgcccactga atcgtacacc acgcgcactt tcttgccacc ggtgatctct
    22561 tttaaccgct cgaccagatt ctcttcacga tagttaataa cctgccatgc gcccgctttt
    22621 agcgcactct gcgctttttg cgcggttccc acggtgccga taagtttcgc gcccagggct
    22681 tttgcccact ggcaggcaat caagccaacg ccgccagccg ctgcgtggaa caggaactgt
    22741 tcatcgggtt taatttcata ggttttgcgc agcagataat aaaccgttaa gcctttcagg
    22801 aaggatgccg cagcttgctc aaaagaaatt gccgcaggca gaatcgccgc tttatccgca
    22861 ttaatgttat gcatagagct gtaagcgcct aatgccgatt gtgcatagac tacgcgatcg
    22921 cctgccttaa tatgctttac accactgccg actttactca cgatgcctgc cgcttcggtg
    22981 cctaatccgc tgggtagcga tggtggcggg taaaggccgc tgcggatata cgtgtcgata
    23041 aaattgatgc cgatggcttt atttttgacc tggatttcat tctccgccgg atcggcagga
    23101 gtgaactcca cggcctgaag tacttccggg ccaccgtgct tgtgaaattc gatgcgcgtt
    23161 gccatttaaa ctcctttact gagcgacata tctgttttaa gcctaacagt agtcgccagg
    23221 ccgcgcgggg attttttccg ggctgcggtc acaacccgga atatctctga tcagtattga
    23281 aacggcgtcg ccgcaatcca gttttgccac tggcgctcaa agttggttag cgttaatacg
    23341 ccccgttcac ggcacgggcg agtccaggtt tggtaatcat tagcttcccg acaaacacaa
    23401 atcatatatt gcctggcatc ctgattttct ggccgaaaac gccaggccag gagtggccca
    23461 cctggtgttt gcggtgcaat accggcttcg ccatcataga tagccctggc accacgactc
    23521 ccaccaccgt tttcgacata acattgtaaa gaacgcagta cagccagcgc cagttgcgca
    23581 ctttgccgcc actggaaaga ttgtgccagg ctgttttcat cgcattgaat accttcccgg
    23641 cgaatatctt cacaaagtgt tgccagggtt tgcgcggcag tgttgatccc ctgtgtatga
    23701 cacaaaattg cggcatggtg gctcatggta ttttgtatcg tttctcgcac gtcgtcacgt
    23761 agcagcccct gattcaggta ctgttgcgcc tcattaatcg tactgagaat ttgttgcctc
    23821 tcgagaggcc ggtcctggct tttctgatga gcaatatgta atgcacagcg gcgggcgaaa
    23881 acttgcccgg cattgagcgc tgctccacct gggcgcgtga caccatgtgt tccggcattt
    23941 tctccggcag cataacaacc agttagcgat gtacgtcccc aaatatcaac ttcaattccg
    24001 ccattaagat gttgattatt tagcgtaaac ttgagcggtg aggctgtgag atcatgaccg
    24061 tgcatctgat acaaacgaat agccagagga ttcatctgtg ttaatctcgc cagcggttgt
    24121 gccaacaggg cgtgattttg ttgcagatag tcgatgacat catcatccag ttcttgcagg
    24181 ttgaatgacg ttccgtccgc tgcgggttct ggttcccgta gaaaatcaag aaaaacatcg
    24241 cgcccttttt gagtctcttc ataaactgcg acatccagca ggctggagcc atattccagc
    24301 attcgttccg cgtgaaacgg ccactggtaa cctttacgaa atattgcgga ggccagcata
    24361 cgagtcgtgg ggtaataagc tgccagaaaa ttatactgac gcccggagtg gtcctgcgaa
    24421 tagatgcgcg gcatcgcctg catgtaagta ccggaaagat tccacggaaa ctggctgcgc
    24481 ggtgtaccga taccaaactg actttccgta agattaacca gagttatacc tgcttccagt
    24541 gccatgccca acgcactaaa acagttcacc ggataaacac tgtcacgata aagttcacct
    24601 ggtccgcccg tggctaaaac cagatgctgg cagcgaatca cgaccattcg ccagggatta
    24661 tcccgacagg ctttatcgat ggcgatcacc cccacgactc gtcgattttc cccttcacca
    24721 ctggtcagga tatgaatcgc cgttgtgtga tcgcaaagag gaatattaag gcgcattgcc
    24781 tcctgggcca gcaccttcac catcaggcgc gatgttcgag gaccgcagct agtggcacgg
    24841 ccaaattcat cgtgatcggt ttgataacgc aacgttgcgc caaagagatc ttcaggcaga
    24901 tccagaccca gatatttcag tacttcacag gtgttcaccg atccgaccgc ctcaacatat
    24961 gcggtgtcat gatccatcgc accacctgcc gccagggtgt tacttaatgt cagaaaatgg
    25021 tcaccatttg cacgcgtatt cgcggtgtga agcgtttgtt tatcggaacc agaacaggcg
    25081 gaagtgcccc agaaggcttt gctggaggcg accattacat cgacattttg ccgtttcagc
    25141 tcgacggcag ctcgccatcc tgccgcgcca ctgcccagaa ccagcgtgtt gcagcagtac
    25201 tctggtaaaa tggtaccgtt aaggtcgata ctaccgctgg cttgagccag cggtgcgtaa
    25261 tggggcattt tgatatcagt gagcgccgtt agcgtttcgt caatcattgc gtcccaccta
    25321 tttcatcaga ctttcgatta agccgctcat tgccatatag cccatgatta gcgtaaatac
    25381 gcttatggca ataaacatga cgttttccca gcgtgagcaa cgatatttgc ccattaacga
    25441 aggcttgttc gccagccaga acatacataa aacagtcagc ggcagaataa tggcgttaaa
    25501 ggcctggctg gcaatcatca ccaataccgg gcgtgcgtgg aacagcggca caaccagaca
    25561 aagcagagac ataaccgtca tcaggatacg gtaacctggg ctaacttcgt tgctggtatc
    25621 acgccagtca gccagcagcc agggcgttaa cagcaaattt gggaactggc tggaaagccc
    25681 ggctgcaaca ataccaataa caaagagccc cacggcagct gagccagcaa taggttcgag
    25741 aataatgacc atttgcgagg cattatttaa acctgtgcca gtttggtaca acgtacccgc
    25801 agccgcagcc atcaccgccg cactgatgat gaacatcatt cctgcactga caatggcatc
    25861 acgattttga tgaggcaaat tgtccacacc ccaaccatta ctttttacca gggtcgtgcg
    25921 cataagaaac attgctggtg ctacggtggt acccaccatg ccagaaatta tcagaaatgg
    25981 cgtactgtta tcgccagcga caggatcggg aacagacgga ataaatccgg ccagcagatc
    26041 tgcgaccggc ggcatcataa tgaaaaaatt aatcacaaac gccagcccca taatgccaac
    26101 cagcgcggag aggattttgt cgaacccggc gttgctgttg gtccagagca tcacgttaat
    26161 cataatgacc atcgcgatag cccaggcgag agctgagata ccgccactaa accaggtcgt
    26221 ggaccatgtt gccattacat ccgccagaat acccattacc cccataatgg aaccgctcac
    26281 ggcaacggtt aacccgatca gaaacaacat cgcaacgccg ggatggatat ggcgacgaaa
    26341 tgcagtcaac gcgcgttcac cagtgacgga agcataacga ccataaatac ggattaaaaa
    26401 ccaggtaaca atgcaagaaa gcgccacggc ccacatgagc gagacaccat agttagcacc
    26461 tgctttagcc atagtggtga tgctgcctgt tccgatgctg tagccaataa gaaagatgcc
    26521 aggtgcaaag gccgcgagtt ttgtcattat tcgccgaaat aaagaaggcg tttggggaac
    26581 agaggttgtc atattcatta tctttctttc cttgtaagag gcgatcagcg tgcctgaaag
    26641 tgcggttgac gcttctcagt aaatgcctga ctgccttcat gagcatcttc agtcgtggcg
    26701 gaaaaagcgc cagccagtgc ttcaagcgtc gaggccacag caataccttc tccggcatca
    26761 ataacctgct tggcgatctg cactgcaacg ggagcacgct gggcgatacg ttgtgccagt
    26821 tgcagtccgg cgtcgtgtaa cgcctccctg gcgacaacgc gattcaccag cccgcaggac
    26881 agtgcccgct gtgcatccag tggttcaccg gtaaatacca gttctttcac cagtgagcgc
    26941 ccaagcagtg ccgtcagccg ctgcgttcct gaccagccag gcactgtcgc cagcccggtt
    27001 tctggtagtg ccatttgtgc ttcggcgcta caaatacgga tatcggcggt agcagccagt
    27061 tccagaccac cgccataagc caggccgtgc aacagggcga ttacgggctg tggcagttgt
    27121 gccagagcat caaatgcctg atgaccgcgg cggatccagt tgcgccacat atcgagtgca
    27181 gaaagagagg tccagcgatg aatatccgcg ccgacacaaa acgctttttc actggcactg
    27241 gtcagcagta ctacgcggat ctccgaatca gtttccaacg tgtggcaatg cgccagcagt
    27301 tgttcgagca tctctggggt taatgcgttg agtttcgccg ggcgattaat caccaaagtg
    27361 gcaatgaaac cgttgcgctg gaggtcaatg gtgccgttat tcatggtgac ttccttgttg
    27421 aaaatagagg ggattcatta gtttgagatc gtctgccaga cggagtgggg catcgctttg
    27481 ggcgagaatg tcgcgttcag gatcaatacc tggggcaatt tctgtgacca gtagcccgtc
    27541 tggggttaat tccagaacgc agcgttcagt aatgtactgt acttgttgcc cgagtcttat
    27601 tgcgcgtttg ccggagaacg tcacgtgggc gacgtcctgt accagttttt tggctttgcc
    27661 ttctttaatg atacgcagct gaccctcttc cagttgcagt tgggcaccgg cattgaaaaa
    27721 gccagagaaa ataatgcgtt tcgcatgtga ggtgatatca ataaatccac cgcatccggc
    27781 agtgacatgt ggacgagcgg ggagatgtga gacgttcact gagccatctg cgccgatttg
    27841 taaaaacgac atcagagtga gatcaaaacc accaccctgg aaataggtga actgctgggg
    27901 agagggcaaa aatgcttctg cattagaggc acaaccgaac tgaaattcca gcagtggaac
    27961 tccgccaatc gcgccctgtt ccagcagcca ggtgacgtca ccgtggcgac cttgttccag
    28021 taaaatgcgt ggtacgttgg cagaaatacc aaagccaata tttaccgcat ctccactttg
    28081 tagttcttgc gcgactcgct gggcaataac tcgcgccggg ccatgttcca tataacgaaa
    28141 ggctgacagc ggacgactga tttcgccgga aatggcaggt tcatattgcg tttgtgtcgt
    28201 ttgtgtttgt tccggcgcaa tcacgatgta atccaccagt acacccggaa tacgcacctc
    28261 ttttggtttc agacttccgg cggcaacctg tcgttttact tgcgcaatta tgatgccacc
    28321 attattgcga acggcagtag cctgttccag tggccccaga taagcccctt catgttcata
    28381 agtcaggttg ccgcgttcat cggcagtggt agcgcgaatg atcgccacct gcggcacgat
    28441 actggggaaa aacagccagt cgtcgttagc aaatcgaaca cgttcaacca gagattgttg
    28501 cgccgctttt tcattcattg caccgccctg caacagggga tccacatagg tatccatgcc
    28561 tgtttgtgtc agcacgccag gacgatgagc cgccgcttcc cggtgcatat caaataagat
    28621 cccggaaggc agattccagg cggggatcgc gttgtcattg atcatttgcc agattaacgg
    28681 aggcgtcatt gatgatggac cggaggggta ggagccagcg atcacttttg ccagtaagcc
    28741 cggttgcgcc agatgatcga tgccttttac gccatacata tcaccagcgg cgatagggtg
    28801 gatggaggtg atgttttgcg gatgtccggc acgttgaaag cgttcaccta ttgctgccag
    28861 caccgcatcc ggacacccca ttccactgga ggaggagacg gtgacaactg cgccatcaac
    28921 aatcaaatct gcagcttccg gtgcagtgat cgtgcgtaca gagcacataa taatttcctt
    28981 gtttcgatat aaaaataatt aaaaatatac atttaaaaca atatgataat tttcataaat
    29041 taattggaac gatctaattt atgcgtaatt tgtacgatta gggtgtaaaa aacacaacaa
    29101 gggaagtgcg aatctgtgat cgaaacgacg atctcctgcg atcccttgcg ttaacggatc
    29161 gcatgagtaa aaggagatta tgataaagta attgccttga tcttatgggg gtttggagca
    29221 ggcatggcga agtcggatac aggaagaaag cgggtaacat taaccgatgt tgcgcgtgcg
    29281 gctggcgttt cgaaatcgac tgtatcgctg gttctcaacg acagttcact tatcaaaaaa
    29341 gagacgcaac aaaaagtcca gcaagccatt gaacaattag gctatgtcta taaccgtttt
    29401 gctgccaatc tgcgttcgca aaaatcgcta actatcggcg tagtcattga cgatttgata
    29461 aatcctttct tcgctgaatt tactatgggt cttgagatga cgctggcgga gcacggtttt
    29521 atcaccgtga tgtccaatac ctcccagcgt agtgaccggc aaaaacaggt gcttgatacc
    29581 ttgctggaac accacgtcgc gggcattgtc ctctgtccag tgaacagtac ctcagaagcc
    29641 gatttacagc gttatgccaa cagttccaca cccttattaa tcacgatgcg ccctctggat
    29701 tggcaacaat tgcccgtcga ttacgttggc gtggacagcc acgcaggtgt acgggaagcg
    29761 acggaatatc tcattcaaca agggcacaga gatatcgtgt ttattggtgg tcttacgcac
    29821 catatgcgtt atcagggata ccttgaggcg atgaatcacc atggtttaca gccgtggagt
    29881 actgatgctt tttcgctgcg tgctgaaccg actcaggcca acggctacca gttgatgcaa
    29941 cagctgttgg atatgccttc gccacccaca gcggtgattt gttataacga tttaatggct
    30001 tttggcgcgg agtcggcact aggcgaaaga ggattatttg ccggggaaga tatttcgttg
    30061 attggtaatg atggcgtggc ggcttgtgct tacagtaacc cgccgttaac cacaattgcg
    30121 gtagaaccat tggctctggg gaaacaggcg gcgcagcaaa ttttacggcg tattgcgcaa
    30181 ccagacgcac cgctcagcca ttacatctat cgcccgaccc tgcaaattcg cgcttccacc
    30241 ggaaagcggg ggcgttaaat gctgtagcga gaaactgttt ctcgctatac tctcttgtcc
    30301 cccattcaac agcaggtaac accattcgtt atggcaggca atagaccttt caacaaacaa
    30361 cagactgata accgcgaacg cgatccacaa gttgccgggc tgaaagtgcc tccgcactcg
    30421 atcgaagcgg agcagtcggt gttgggcggt ttaatgctgg ataacgaacg ctgggatgat
    30481 gtagccgagc gtgtggtggc agacgatttt tacacccgcc cacaccgtca tatctttact
    30541 gaaatggcgc gtttgcagga aagcggtagt cctatcgatc tgattaccct tgcggaatcg
    30601 ctggaacgcc aggggcaact cgatagcgtc ggtggttttg cttatctggc agagctgtca
    30661 aaaaatacgc caagtgcggc gaacatcagt gcctatgcgg atatcgtgcg cgaacgtgcc
    30721 gttgtccgtg agatgatctc cgttgcgaat gagattgccg aagccggttt tgatccacaa
    30781 gggcgtacca gtgaagatct gctggacctt gctgaatccc gcgtcttcaa aattgccgaa
    30841 agtcgtgcaa acaaagacga agggccgaag aacatcgccg atgtgctcga cgcaaccgtg
    30901 gcgcgtattg agcagttgtt tcagcagcca cacgatggcg ttaccggggt aaacaccggt
    30961 tatgacgatc tcaacaaaaa aaccgctggc ttgcagccgt cggatttgat catcgttgcc
    31021 gcgcgtccgt cgatgggtaa aacgacattt gcgatgaacc tcgtcgaaaa cgcggcgatg
    31081 ttgcaggata aaccggtact tatcttctcg ctggagatgc catcagaaca gattatgatg
    31141 cgttctctgg cgtcgctgtc gcgtgttgac cagactaaaa tccgtaccgg gcagctcgat
    31201 gatgaagatt gggcacgcat ttccggcacc atgggtattt tgctcgaaaa acgcaatatc
    31261 tatatcgatg attcctccgg cctgacgcca acggaagtgc gttcccgcgc acgccgtatt
    31321 gcccgtgaac acggcggcat cgggcttatc atgatcgact acctgcaact gatgcgcgta
    31381 ccggcgcttt ccgataaccg tacgctggaa attgcagaaa tctcccgctc gctgaaagca
    31441 ctggcgaaag aactgaacgt gccggtggtg gcgctgtccc agttgaaccg ttctctggaa
    31501 caacgtgccg acaaacgccc ggtcaactcc gacctgcgtg aatctggctc tatcgagcag
    31561 gatgcggact tgatcatgtt tatctatcgt gatgaggtgt atcacgaaaa cagtgattta
    31621 aaaggcatcg cggaaattat tatcggtaaa caacgtaacg gcccaatcgg gacggtacgc
    31681 ctgaccttta acggtcaatg gtcgcgcttc gacaactatg cggggccgca gtacgacgac
    31741 gaataataat tattttatga attaggtaat taaagcaaac acttatcaag gaacacaaat
    31801 gcaagcggca actgttgtga ttaaccgccg cgctctgcga cacaacctgc aacgtcttcg
    31861 tgaactggca cctgccagta aaatggttgc ggtggtgaaa gcgaacgctt atggtcacgg
    31921 tcttcttgag accgcgcgaa cgctccccga tgctgacgcc tttggcgtag cccgtctcga
    31981 agaagctctg cgactgcgtg cggggggaat caccaaacct gtacttttac tcgaaggctt
    32041 ttttgatgcc agagatctac cgacgatttc tgcgcaacat tttcataccg ccgtgcataa
    32101 cgaagaacag cttgctgcgc tggaagaggc taacctggac gagccggtta ccgtctggat
    32161 gaaactcgat accggtatgc accgtctggg cgtaaggccg gaacaggctg aggcgtttta
    32221 tcatcgtctg acccagtgca aaaacgttcg tcagccggtg aatatcgtca gccattttgc
    32281 gcgcgcggat gaaccaaaat gtggcgcaac cgagaagcaa ctcgctatct ttaatacctt
    32341 ttgcgaaggc aagcctggtc aacgttccat tgccgcgtcg ggtggcattt tactctggcc
    32401 acagtcgcat tttgactggg tgcgcccggg catcattctt tacggcgtat ctccgctgga
    32461 agatcgctcc accggtgccg attttggctg tcagccagtg atgtcgctaa cctccagcct
    32521 gattgccgtg cgtgagcata aagccggaga gcctgtcggt tatggtggaa cctgggtaag
    32581 cgaacgtgat actcgtcttg gcgtagtcgc gatgggctat ggcgatggtt atccgcgcgc
    32641 cgcgccgtcc ggtacgccag tgctggtgaa cggtcgcgaa gtgccgattg tcgggcgcgt
    32701 ggcgatggat atgatctgcg tagacttagg tccacaggcg caggataaag ccggggcccc
    32761 ggtcatttta tggggcgaag gtttgcccgt agaacgtatc gctgaaatga cgaaggtaag
    32821 tgcttacgaa cttattacgc gcctgacttc aagagtcgcg atgaaatacg tggattaata
    32881 ttcgtctcca gggagggaca atgtcttatt cagaaatcgc cgcgtgtctg gcatacgcgg
    32941 tttccgtctg gctggctgcc agaaacaatg tgcatacatg gtggatcggc ataattggca
    33001 gcatattgta cggctgggtt ttttggtccg tgcaactcta tgccgacgtt acgctccagt
    33061 tattcttcat cgtgaccagc atcactggct ggatccactg gctgaaaggt cagggtggcg
    33121 acatcttgcc ggtgcgccga acgcaagcca gtcacttttt ccttttgttg ctctgtgctg
    33181 tcgtcgtggc aggcggttac ggctttttac tccacacctt taccaatgct tgggcaccct
    33241 ggctggattc gttgattttg accttcagcg ttctggcaca attcatgttg atgggaagac
    33301 gtatcgaaaa ctggtacgtc tggttagcgg tgaataccct ggcggtgcca ctgtatatga
    33361 cgcgcggttt aaacctgacc gctggcttat atttcctgtt ctggattaac gcctggtatg
    33421 gtttgtatca atggcgcaaa gagttgcaaa catcatgaag ccttttgcaa cgggtctggt
    33481 ggttggtaaa ttcgcgcctt tgcattgtgg tcatgaaaag ttgatcaata ccgcactggc
    33541 gcaatgtgaa gaactgttca tcatcagtta ttccgtgccg gaaatgcctg actgtgaacc
    33601 tgaaaagcgc ctgacgtggc tgcaagtgcg cttcccgcaa gcgacgtttt tggtactgac
    33661 gccagagctt gttgcgcgtt ataacttacc tgctatcccg cataacaatg ctgatgccga
    33721 catccaccgt cattatgtgg cgacgctatg cttgcagata ttacgttgcc gcccacacgc
    33781 tgtatttact gcagaagact atggcgatgg ctttgctaac gtgctggcgc ggcgttttgc
    33841 ccaacccgtt gaacacgttc gaatggcgcg cccagttggc gatgaagcgc cttcgggaac
    33901 gcttattcgt tccgatgttc accgctatcg ctacatgttg gcgaatgatg tttattacag
    33961 tttcgttcgc cgtatctgtc tgcttggtgg cgaatctacc ggtaaaagca cgctttcaaa
    34021 agcgctggcg ggcgtgcttg atacggtgta tgtcgctgaa tttggccgcg actattggga
    34081 agaaaaaaac ggtatcttaa ctgcggatga tttgttgcat atcgcctgcg aacaggtacg
    34141 ccgggaacaa caagcagaag ctaatcacta tcttatatgt gacacgtcgc cactcacgac
    34201 cttattctat gcccttgatc aatatggtca tgctccgcaa gaacttcacc aactggcgga
    34261 gcgggaatac tctctggtag tgctttgcgg cgcagaattt cccttcgtgc aggatggcac
    34321 ccggcaagga gaggtatttc gcgcacggca acaggtgtgg tatgaacagg aattgtcgcg
    34381 caggaacatt ccttatcttt cggtttcggg gccactgcaa gagcgagtgg agcaaattct
    34441 tcgccaactt cccgactgac tttgaacatc ctctcgatct tcgccttctt ccggtttatt
    34501 gtggtttaac cacctgcccg taaacctgga gaaccatcgc gtgtttcaaa aagttgacgc
    34561 ctacgctggc gacccgattc ttacgcttat ggagcgtttt aaagaagacc ctcgcagcga
    34621 caaagtgaat ttaagtatcg gtctgtacta caacgaagac ggaattattc cacaactgaa
    34681 agccgtggcg gatgcggaag cgcgcctgaa tgcgcagcct catggtgctt cgctttattt
    34741 accgatggaa gggcttaaca gctatcgcca tgccattgcg ccgctgctgt ttggtgcgga
    34801 ccatccggta ctgcaacaac agcgcgtagc aaccattcaa acccttggcg gctcaggggc
    34861 attgaaagtg ggcgcagatt tcctgaaacg ctacttcccg gaatcaagcg tctgggtcag
    34921 cgatcctacc tgggaaaacc acgtagcaat attcgccggg gctggattcg aagtaagtac
    34981 ttacccctgg tatgacgaag cgactaacgg cgtgcgcttt aatgacctgt tggcgacgct
    35041 gaaaacatta cctgcccgca gtattgtgtt gctgcatcca tgttgccaca acccaacggg
    35101 tgccgatctc actaatgagc agtgggatgc ggtgattgaa attctcaaag cccgtgagct
    35161 tatcccattt cttgatattg cctatcaagg atttggtgcc ggtatggaag aggatgccta
    35221 cgccattcgc gccattgcca gcgctggatt acccgctctg gtgagcaatt cgttctcgaa
    35281 aattttctcc ctttacggcg agcgcgtcgg cggactttct gttctgtgtg aagatgccga
    35341 agccgcaggc cgcgtactgg ggcaattgaa agcaactgtt cgccgcaact actccagccc
    35401 gccgaatttt ggtgcgcagg tggtggctgc ggtgctgaat gacgaggcat tgaaagccag
    35461 ctggctggtg gaagtagaag agatgcgaac tcgcattctg gcaatgcgtc aggaactggt
    35521 gaaggtattg agcacagaga tgccagaacg caatttcgat tatctgctta atcagcgcgg
    35581 catgttcagt tataccggtt taagtgccgc tcaggttgac caactacgtg aagaatttgg
    35641 tgtctatctc atcgccagcg gtcgcatgtg tgtcgccggg cttaacacgc aaaatgtgca
    35701 tcgcgtggca aaggcgtttg ctgcggtgat gtgattttaa tcaacaggcg gatctctccg
    35761 cctgttcctg actccagcca ctccctacta ttttgtccct tttttcgccc acttttacat
    35821 catctctttt ctataagttt ttgaaaatta gtgtttttaa ctgttggtac ggattgtgca
    35881 gtaatgcagc tgtctatgac catcaggaga aaaacatgga gaacatgacc tcgcccggca
    35941 cgctgcttag tggagataac gcgacctggc ttgaagagta ctaccagacc tggttgcgta
    36001 cgccggaaca actcccggaa gactggcggc gtttttttct ttcacctgaa ctgactgtcc
    36061 aaagcgtatc cggtgataac aatatcagcg gtgcgacctt aaaaaaacag gctgcggtaa
    36121 tacaactgat caatgcctgg cgcacgcagg ggcatctgcg cgcaaagctt gatccacttg
    36181 gtttgaatcc cccggctgac gttccctccc tccagcctgg tttttgggga ttaagcgaag
    36241 aagacttgtt gcaggagttt agcgtcacct ttggcgcaca caccacgcaa atgccactta
    36301 agcaactgct taacctgctg gagcaagcct gggccagtag ccaggcttat gaacttgctc
    36361 atctggaaaa tcgtgaagaa atcaactggt tgttatcacg gattgagagc agcaatgcgc
    36421 cacaagctga tgcccaaacc tgtattgctc gttttgaaaa actgatggcg gcagagacgt
    36481 tagagcgtta tctgcatacc cgttatgttg ggcagaaacg gttttctctg gaagggggcg
    36541 aaagtgcaat cccggcgctc gatacgctga caaaacgcct gcgtgcgcaa ggtgtggagg
    36601 aaatggtcat tggcatggcg caccggggaa gacttaatgt tctggttaat ctgttgaata
    36661 aagacccggc acagcttttt gctgagtttg aaggcaagca gacgatcggc agtggttcag
    36721 gcgatgtgaa ataccacatg gggtattcca gcaatctgga aacacctgcc ggttcgctgc
    36781 atgtggcgct ggcgtataac ccctcacatc tggagatcgt gaatccggtg gtgctgggcc
    36841 aggtgcgagc caggcaggaa cgccgtggtg aggatggtca ggcaaaagtg gtgggcgtac
    36901 ttattcatgg ggattccgcg ttgggcggcc tcggcgtcaa ccaaaccacg tttaacttat
    36961 cgcagactca gggctacggc actggcggta cgctacatct ggtgattaac aatcagattg
    37021 gcttcaccac ttcccgcttg caggatatgc gttcttcacg gtactgcacc gatattgcca
    37081 aaatggtagc ggccccaatt atccacgtta atggcgatga cgttgacgct gtttgccagg
    37141 taatggaact ggcgtgcgaa tggcgagaca ccttccggcg tgacatcatc atcgatattt
    37201 gctgcttccg taagcacgga cacaacgaaa gcgatgagcc acgtcttacc cagccacaaa
    37261 tgtatcaggc agtggatgct caccctggta cgctcgcccg ttatggtgaa tctcttgccc
    37321 gccgcggcct gttaactcag gcgcaacaag atgagatgac ggcacgctat cgcgactggc
    37381 tggatagctg ccagaaacgt gaaccgcagc ctctcaaacc tgcaattcac tcttttagcg
    37441 ctaactggta tgggttgacc aaccctcact ggagcgcccc ggtatctacg gcattgcctc
    37501 ggcaaaagct ggcagcgtat ggtgaaatca tttctacact gccacccgat gtggtcgcac
    37561 atcctaccat taaacggcaa ctggcgttac gccaggatat ggccgcaggt actcagccag
    37621 tagactgggg aatggctgaa atgctggcct atgcatcgct tgtggatgcg ggtgttggtg
    37681 tgcgtctttc tggtgaagac tcagggcgtg gcacgttcag tcatcgccat gcagtggtac
    37741 atcatcaaac ggaagcccgt cgttatctgc cgttacagca tatccgcgct ggtcaggcat
    37801 ctttcgatgt ttatgattcg gtgcttaatg aagaagcgct cttagccttt gaatatggct
    37861 attcaacgtc tgcgcctcag caactggtta tttgggaagc acagtttggt gactttgcca
    37921 acggcgctca ggttgctatt gaccaattta tctcgtctgg cgaaaccaag tgggatcgct
    37981 atagcggttt aaccattctt ctgccgcatg gctatgacgg acaagggccg gagcactctt
    38041 ccgcacgtcc tgagcgctgg ctgcaactgt gcgcagaaaa caacatgcag gtggtgatgc
    38101 ccagtgagtc agcacaaatg ttccatttgt tgcgcggtca ggcattacgc ccaatgcgca
    38161 aaccgttagt gataatgatg agtaagcgtt tacttcggtt taaaggcgcg atgagcgagc
    38221 tttgtgaatt cactgatgac gcttataaac cggtaatcac cgatccgcaa ttgcatcagt
    38281 cccagaaagt taagcgcgtt attttgtgta gcggccaggt ctattacgac gtactggaag
    38341 cccgtaagca gcgtgagcat gaagacgagg tagcgattgt tcgcctggag caactctacc
    38401 cgttcccggt cgcagaactg aatgatgttc tggcgagttg gccgaactgc tgcgaatgga
    38461 tttggttgca ggaagagcca gaaaaccagg gcgcgtggcg gcagattcgt catgagctgg
    38521 cggcactgaa aattaacact ccttactggc agtatgcagg ccgcccggca gcggcagcac
    38581 cagcgacagg gtatgggcgt gtacataaac aacaaatcga cgaatttctt gctgctgcgt
    38641 ttgcagatat tcagccatga aaggcgcaca aaaataataa ggagaacatt ttatgatcga
    38701 aattactgtc cctgtattac cggaatctgt cacggaaggc acgctgacaa cctggtgtaa
    38761 acaagaaggc gagcacgtaa aacgcgatga tgtgattgcc gagctggaaa ccgataaagt
    38821 cattctggaa ataccggccc cgcacgatgg cgtgttaagc aatattatcg tcagcgaagg
    38881 tagcacggtc acgtccgcgc aactgttggc gcatcttaag ccgcaagctg tcatagaaga
    38941 aaccgtcaca ccagtcaccg aaaccctggc gatgccttcc gcacggctgg aggcacagcg
    39001 tagtggtgtc gaacttgctg acgttgcggg gagcgggaga aatggacgta tcctgaaaga
    39061 ggatgtgcag cgcgttactc ctgcaccagc cattcagcct gaacgggtgg cggagatcgc
    39121 gccagctaaa cccttgacac cgggtgctcg tcaggaacgt cgtgagccga tgtcgcgctt
    39181 acgtcagcga attgctgagc gtctgctggc ttcccaacaa aataacgcca ttcttaccac
    39241 attcaacgaa gtaaacatgc agagcgtgat ggacttacgc gcccgctgga aagatcgttt
    39301 tgccgagaag cacggcgtga agctgggctt tatgtccttc tttgttaagg ccgttacccg
    39361 ggcgctggag cgcttccctg tagtgaatgc cagcgtcgat ggcaacgaga ttatctggcg
    39421 tgattattgc gatatcggca ttgcagtgag cagcaaccgc ggcctggtag tgcctgtgtt
    39481 acgcaatgcg caatcactct ctttggtgga aattgaacgg caaattgccg aatacgccac
    39541 acaggcacgc aatggcaaat tgccgctgga ggcgttacag ggcggcacgt tctctatcac
    39601 caacggcggt acttttggtt cgatgatgtc aacgccaatc attaacccgc cgcagtcggc
    39661 cattcttggg atgcatgcca tcacgccgcg cccggtcgca gaaaacggtc aggttgttat
    39721 tcgtcccatg atgtaccttg ctctcagcta tgaccatcgc attatcgatg gtcaggaagc
    39781 cgtgcataca ttggtggcaa tacgtgagct actggaatca ccggaacaac tgttgctgga
    39841 tctttaagga gggcgccgat gagtacaatt tttgatgtgg cagttatggg ggggggccag
    39901 gtggttacgt agcggcactg cgggctgcgc agaatgggct ttccgtagta tgtattgatg
    39961 atggtgttaa tgcacagggc gaaccttcgc cgggcggtac gtgtctcaat gtcggctgta
    40021 ttccgtcaaa atcgttgctg caatcttccg aactgtacgc gcaagtgcag catgaggcga
    40081 gcattcatgg cgttaatgtt gacggcgtat ctttcaatgc agctgcgatg attcaacgta
    40141 aagacgccat agtaagccgc ctgacaatgg gcatcagctt gctgtttaag aaaaataaag
    40201 tgaagcacct gtgcggcctc gcaacgttag aatgtgctca ggatgagatt tggcaattac
    40261 gtgtaaacga tcaacacctt cacgcccgta atgtagtgat tgcaacaggt tctcagccgc
    40321 gtcagctgcc tggcgttacc attgataatc aacagatcct cgacaatcgt ggcgccctgg
    40381 cattgagcga agtgccgccg cgtctcggtg tgatcggcgc tggcgtgatt ggccttgaac
    40441 tggggtctgt gtggaatcgg gttggttcag acgtcacgtt gctggagatg gcgccaacgt
    40501 tcttgcctgc actggaagct cgcctgtcga acgaagtccg taaagcaatg attgccagtg
    40561 ggatgaaaat gcaactggca gtagagattg aggctattga acaacgggat gacggtgtgc
    40621 atgtgcgctg gcggcagggt gagaagcgag aagaaagccg cttcgataaa ttaattcttg
    40681 ccattggacg tgtaccgcga ctttctggtg tcgatctcgt gcaactcggt cttgaagccg
    40741 ataaccgtgg cggtattgcc gtcgataact tatgccgtac cgggaaagcc gggctgtggg
    40801 caattggtga tgtcgtgcga gggccgatgc tggcgcataa agcgatggct gaaggtgtag
    40861 tggtggctga tcaaattgcc ggactggtgg tagagccgat taattttgcg ctgatccctt
    40921 cggtgattta cacccaaccg gaagttgcgt gggtagggga aaacgaggcc tcactgaaag
    40981 ccgctggcag agtctttaac aaaggaaatt ctctgtttgc cggtaacggt cgcgccttag
    41041 cattaggcca ggaaggtggg cgttgcacgt tatacagtga taaacacact gaccgcgtac
    41101 ttggcggagc gattgttggc ccgcaggcat cggagctgat taatgaaatt gcgctggcga
    41161 tgacttttag cgcctctggc gaggatattg cctgtgcgat ccatgcgcat ccgacattaa
    41221 gcgaagtcat ccacgaagca gcaatggcct tgaataataa agcattacac ggataacaca
    41281 caccactgag ggaatatatg aatttacatg agtatcaggc caaatcgtta ctggctggca
    41341 tggggatgcc gtgtccaaaa gagatagcta tccaacagat ttcgcaactg gcagatgcct
    41401 ggcaacatat tgcttgcccg agtaaaggcg cggtacttaa agcgcaggta catgctggtg
    41461 ggcgcggtaa agctggcggc gtaaaagtgc ttaagcagct gccggaagca caggcttttg
    41521 tacagcaaat gttgggatcg caactggtga cttatcagac cgggccagaa ggacagtatg
    41581 tcagcagtat tttactgtgc gaaaacattt atccggtacg ccaggaactc tattttggca
    41641 tggtggtgga tcgtgaaagc cagcgggtca cgtttattgt cagcccggaa ggtggtgtgg
    41701 aaattgaaaa agtcgcccat gagacaccgg agaaaatcag cagtgtcagc attgatccac
    41761 tgacaggcgt gcagccttgc catatccgcg aaatgtttgc tgttctgcaa ctggaacacg
    41821 gactatttgc tgcctttagt cgcctggtta atcaggcgtg gaaagccttt aacgaactgg
    41881 attttgccct gctggaaatt aaccctctgg tgttgcggga aacgggcgaa tttatgtgtg
    41941 cagacgcgaa agtatcgctg gatgataacg cgctgtatcg tcacccggaa ctccaggtgc
    42001 tacgtgatga aacccaggaa gatccacgcg aaagccaggc agcgaagctt gatcttaatt
    42061 atgtctcgct ggacggaaat atcggctgca tggtcaatgg tgcagggctg gctatggcaa
    42121 ccatggacat catcaagcta tacggtgaac aacccgcaaa cttcctggat gttggcggtg
    42181 gcgcaaccca ggagcgcgtc agtgaagcct ttcggctgat tgtttccgac agcaaagtga
    42241 aagccattct ggttaatatt tttggtggga ttgtccgttg cgacatgatc gccagagcca
    42301 ttattcatgc tctgaatgag gccagcatca ccctgcctgt cgtggtgcgt ctctccggta
    42361 ataacgccgc cgaagggcag cggttactgg cagaaagtgg tctgacggtg gaagccgtcg
    42421 attctctgga tgatgccgcg aaacgcatca ttgcgttact gaattaacag gaggttaaga
    42481 tgagcgtttt gatcaacaaa cacacgcggg tactggtgca gggcattacc ggcaaaaacg
    42541 gcacatttca tacagagcag gctattgcgt atggcacgca gattgtgggt ggagtgacgc
    42601 ctggtaaggg cgggcaacgg catctggatc gcccggtttt tgattcaatg aaagatgcga
    42661 tgcgacaaac tgaggccgat gccagcgtga tttatgtgcc tgcaccgttt gtgctggatt
    42721 ctgttgttga ggctattgaa gctggcgtca aattggttgt ggtgatcact gaaggtgtac
    42781 cgacactgga tatggtgaaa gttcgtcggc tgcttgactg ccacccggat gtgcggctga
    42841 ttgggccaaa ctgcccagga gttatcacgc cgggtgagtg caaaatcggg attatgcctg
    42901 gtgaaatcca ccgcccaggg cgtattggga ttgtttcccg ctccggtaca ttaacctacg
    42961 aagctgtagc gcaaaccacg gcgctgggac ttgggcaatc cacctgtatt ggtattggcg
    43021 gcgatccggt tccggggact aactttattg atgcgctgcg tttattcgaa aatgatccgc
    43081 aaacagacgc ggtaattatg atcggtgaaa ttggtggtaa tgcggaagaa cgggccgcgg
    43141 aatttattcg ccatgatatg aataaaccgg tcgtaggtta tattgccggt gttactgcac
    43201 caaaaggaaa acgcatggga cacgccggag ccattatttc tggcggtagc ggcagtgcag
    43261 aggataaatt ccgtgcgttc gataaagcag gcatggcatg gacaagaaac ccggcgttgc
    43321 ttggcgaaac gttatggaat gttattaagt aatccataaa tgaatatcaa gggagcatat
    43381 cgctcccttt ttttatctgt gtggta
//
LOCUS       DAPQZD010000020        41505 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPQZD010000020 DAPQZD010000000
VERSION     DAPQZD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.020"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..3843)
                     /gene="entF"
                     /locus_tag="RO454_001095"
     CDS             complement(<1..3843)
                     /gene="entF"
                     /locus_tag="RO454_001095"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDY0932963.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFK"
     gene            complement(3840..4058)
                     /gene="ybdZ"
                     /locus_tag="RO454_001096"
     CDS             complement(3840..4058)
                     /gene="ybdZ"
                     /locus_tag="RO454_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDY0932964.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4061..5263)
                     /gene="fes"
                     /locus_tag="RO454_001097"
     CDS             complement(4061..5263)
                     /gene="fes"
                     /locus_tag="RO454_001097"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDY0932965.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     AIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTIGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5301..5501
                     /locus_tag="RO454_001098"
                     /pseudo
     CDS             5301..5501
                     /locus_tag="RO454_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5506..7746
                     /gene="fepA"
                     /locus_tag="RO454_001099"
     CDS             5506..7746
                     /gene="fepA"
                     /locus_tag="RO454_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDY0932966.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7921..8580
                     /gene="entD"
                     /locus_tag="RO454_001100"
     CDS             7921..8580
                     /gene="entD"
                     /locus_tag="RO454_001100"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDY0932967.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPTGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDAGFLRYQIRNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(8582..9517)
                     /locus_tag="RO454_001101"
     CDS             complement(8582..9517)
                     /locus_tag="RO454_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDY0932968.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGITLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(9510..10340)
                     /locus_tag="RO454_001102"
     CDS             complement(9510..10340)
                     /locus_tag="RO454_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDY0932969.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
     gene            10703..11653
                     /locus_tag="RO454_001103"
     CDS             10703..11653
                     /locus_tag="RO454_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDY0932970.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            12004..13524
                     /locus_tag="RO454_001104"
     CDS             12004..13524
                     /locus_tag="RO454_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDY0932971.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            13628..14563
                     /locus_tag="RO454_001105"
     CDS             13628..14563
                     /locus_tag="RO454_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0932972.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            14610..15230
                     /locus_tag="RO454_001106"
     CDS             14610..15230
                     /locus_tag="RO454_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HDY0932973.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGSITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            15232..16776
                     /locus_tag="RO454_001107"
     CDS             15232..16776
                     /locus_tag="RO454_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDY0932974.1"
                     /translation="MLPQEIAPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELN
                     LFPNMNVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQ
                     LVEIARALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELME
                     IGDFITIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTA
                     LHPSGGYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETID
                     KCHFQARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESA
                     SVKEMILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAK
                     TDVYQLVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIA
                     ASTPQD"
     gene            16794..17861
                     /locus_tag="RO454_001108"
     CDS             16794..17861
                     /locus_tag="RO454_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0932975.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            17915..18712
                     /locus_tag="RO454_001109"
     CDS             17915..18712
                     /locus_tag="RO454_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0932976.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLVHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            18745..19530
                     /locus_tag="RO454_001110"
     CDS             18745..19530
                     /locus_tag="RO454_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0932977.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGDGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(19640..19792)
                     /gene="hokE"
                     /locus_tag="RO454_001111"
     CDS             complement(19640..19792)
                     /gene="hokE"
                     /locus_tag="RO454_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDY0932978.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(20142..20294)
                     /gene="hokE"
                     /locus_tag="RO454_001112"
     CDS             complement(20142..20294)
                     /gene="hokE"
                     /locus_tag="RO454_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDY0932979.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            20737..21855
                     /locus_tag="RO454_001113"
     CDS             20737..21855
                     /locus_tag="RO454_001113"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDY0932980.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FISLSAASPYMQGTDTRFASSRPNIFSAFPDNGPMQWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            21921..22169
                     /locus_tag="RO454_001114"
     CDS             21921..22169
                     /locus_tag="RO454_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDY0932981.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            22234..22617
                     /locus_tag="RO454_001115"
     CDS             22234..22617
                     /locus_tag="RO454_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDY0932982.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            22696..23349
                     /gene="nfsB"
                     /locus_tag="RO454_001116"
     CDS             22696..23349
                     /gene="nfsB"
                     /locus_tag="RO454_001116"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDY0932983.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            23457..24704
                     /gene="ybdG"
                     /locus_tag="RO454_001117"
     CDS             23457..24704
                     /gene="ybdG"
                     /locus_tag="RO454_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDY0932984.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(24862..26244)
                     /gene="pheP"
                     /locus_tag="RO454_001118"
     CDS             complement(24862..26244)
                     /gene="pheP"
                     /locus_tag="RO454_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDY0932985.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(26346..29489)
                     /gene="cusA"
                     /locus_tag="RO454_001119"
     CDS             complement(26346..29489)
                     /gene="cusA"
                     /locus_tag="RO454_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDY0932986.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVSDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(29501..30724)
                     /gene="cusB"
                     /locus_tag="RO454_001120"
     CDS             complement(29501..30724)
                     /gene="cusB"
                     /locus_tag="RO454_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDY0932987.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEIDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(30740..31072)
                     /gene="cusF"
                     /locus_tag="RO454_001121"
     CDS             complement(30740..31072)
                     /gene="cusF"
                     /locus_tag="RO454_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDY0932988.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(31096..32478)
                     /gene="cusC"
                     /locus_tag="RO454_001122"
     CDS             complement(31096..32478)
                     /gene="cusC"
                     /locus_tag="RO454_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDY0932989.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDITEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            32635..33318
                     /gene="cusR"
                     /locus_tag="RO454_001123"
     CDS             32635..33318
                     /gene="cusR"
                     /locus_tag="RO454_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDY0932990.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            33308..34750
                     /gene="cusS"
                     /locus_tag="RO454_001124"
     CDS             33308..34750
                     /gene="cusS"
                     /locus_tag="RO454_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDY0932991.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            34900..37137
                     /gene="nrfB"
                     /locus_tag="RO454_001125"
     CDS             34900..37137
                     /gene="nrfB"
                     /locus_tag="RO454_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDY0932992.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            37124..40096
                     /gene="nfrA"
                     /locus_tag="RO454_001126"
     CDS             37124..40096
                     /gene="nfrA"
                     /locus_tag="RO454_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDY0932993.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPASLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WSFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            40097..40987
                     /locus_tag="RO454_001127"
     CDS             40097..40987
                     /locus_tag="RO454_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDY0932994.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISIFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT        10206 a        10902 c        10568 g         9829 t
ORIGIN      
        1 tttaaaatac tccggggaaa taatatccac atgcgcacaa tcctgacgat agatatccag
       61 ctcagcaatc cacggtgccc aggcctgttc aggactcata ccttcctgaa gcgtgcgttc
      121 ggcaacaaat agcgtcgctt taccatcaaa cgctacgcta tgagctgtcg tcagcaggcg
      181 cacagcatca gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag tacttccctg
      241 ctgtgccgcc aggaaggctt cgcgctcgcg gttaatctcc gccagcactt ccgggtccag
      301 accattggct tctttttcct gccagttttg cgtttctggc ggccaggtat ccagcaagcc
      361 aagaaatgcc acctgttcgc cacgggcacg cagtcgcgcc gcaatgccct gcgccagcgt
      421 accgccaagt gaataaccca gcaggtaata agggccgtgc ggttgttgtt caaataacgt
      481 tgccagatgc gcttcgcaga cttcatccag gtttgccgcc gtctgcatgg ggccatgagg
      541 gcgcggcgac tgaatgccga taatcgacca ttgtggatcg agataacgcg agagcacgct
      601 gaactgccag gcaaaaccgg atgccggatg gaaacaaaac agcgtcgggc cattaccttc
      661 acgcaacggc agaatggttt cgaatcccat gcgtcgggtg ctgtcttctt caccatcaat
      721 aatcgttgcc agtttggcga cagttgacgc gaccatcacc tgccccggcg tcacctgacg
      781 ggcaaactgt cgacttaact gcgctgccag tttcattgcc agtagcgaat gaccgccaag
      841 cgcaaagaaa tcagcatcgg catcctgcac gtcacaaccc agcaacgacg agaatgccgc
      901 ggcgataatc gtttcactgc ccgctttcgg cacacgcccc ggcgtttgtg ccttcagttc
      961 aggcaacggt aaggctttgc gatccagctt gccgttggcg ctaagtggta actgtggaag
     1021 ttgcagcaga accaccggta ccatatgtgg tggcaatgtt tctcgaagct gcgcctgtaa
     1081 tgcgctggta tccaacggca gacctgattg cgacaccaga tagcccacca actgacgcgc
     1141 atcaccacca gtggctgccg cctggttaat cacacaggcg tgggtaacgg cttgttcgac
     1201 atccggcagc gcctgcatca cgcgatcaat ttcgcccagt tcgatacgct gcccgcgaat
     1261 ttttagctga tcgtcactgc gcccgaggta ctccactgcg ccgttatcca gccagcgggc
     1321 aacgtctccg gtacggtaca tccgttcacc tggggcaaaa ggatcggcaa taaagcggct
     1381 ggcggtcaga tcgggtcgtc caagataccc ctgcgccagt tgaataccgg tgagatagag
     1441 atcgcccgcc acacccggcg gcaccggatg catcatcgca tcgagaatgc gcaggcccgt
     1501 attccacacc ggataaccaa tcggcacact gctgccgcgc acctctgcca gttcctcgcc
     1561 aaaagccgga taccaactga catctaccgc cgcttccgtc gggccatata gattatgcaa
     1621 cggcgcgccg gttaactgtt gccattcgcg gcataaatca gccggtaggg cttcaccact
     1681 acagaaaacc tgtttcaacg tcgagcaatt ctgacgagcg gtttgcggcg tcagcgatgc
     1741 aacaaatgct gccagcatcg acggcacaaa gtgcgtggtc gttacaccat attcggcaaa
     1801 gaattgctgc atagcgagcg ggtcgcggtg cgcttccggt tcagccatca ccagtttagc
     1861 cccggcaata aacggccaga aaaactccca caccgagaca tcaaaactgc acggcgtttt
     1921 ttgggcaacg acatcttcgc ctgtaagcgg gtagtggttt tgcatccaaa gcaggcggtt
     1981 aacgatagcc gtctgcccga ccattacccc tttcggcctg ccggtggagc cagaggtaaa
     2041 gatgatataa gccgtgtggt gcggttgtga aagctgcagc ggcgcactgc cctgcggtgt
     2101 aagcggggcg ttatagcaaa ggcgggttaa atctggaaca tcggcaaagc gcggcagttg
     2161 atcgtcagtg gtgattaaca gcgacggacg cgcatcttcc agcatcattt tcaggcgatc
     2221 gtccggatag ccggtatcca gcggtaacca ggccgcacca gcctcaacaa ttgcatgtag
     2281 cgcgagagtc aaaaagaccg agcgcggtag agccaccgcc acgctgtcgc ccggtttaac
     2341 gccgcgctca cgcagcagat tcgccagcgc caccacctgc tcgcgcattt cccgatagct
     2401 gaactggtaa cgcgcatctg ccagcgccgg agcatccggt gtttttgccg cttgttctgc
     2461 caccagcgcg ctcagcgtgg tttctggaat ctcaatctga gtggcgttga tctgcgccag
     2521 ctgcgcatac tcacctggca gcataatatc gacatcgccg cacaacagcg ccggatccgc
     2581 ggcgaactgg gcaatcagca ttttcaggcg ttcagcgtgt tgaattaacg ttggctcatc
     2641 gtaacgctgt ttattggcga ggatttcaat actcaaatct ccgtgttcat ccgggaacag
     2701 ggccagttca aggtcattaa ccggaccggt tgccagggga tgggtttgcg cctgaacacc
     2761 aggaatatcc agttggtaat caaatacctt gatattgagt accggaccga acagcggttc
     2821 ctctcctgcc gctcgcccac tgtcacggac aatttgttcg gcgtcgtagc gttgatggcg
     2881 gcgcattttt ttcagttgtg ctgccagtcg ggttgccagt tgcggcagcg tttcttgcgc
     2941 cgcaatgtga atacccaacg gtaaaacgtt gagcacgggt ccggtagccg tcagcgccgc
     3001 cgagcccagt cgacgcataa agataaatcc ggcagcgtag tccatgcggt tgcacaatcg
     3061 ccccagccac aaggctgcca acgcaagtgc taaatcggta cgctgcacac ctgaaagttg
     3121 cgtagccagc tggcggaatt ccccgtcggt aaattccagt ttcaggcgca aaatatctgc
     3181 cgaagcgctg cgccccggta aaggtgccgg agaaagtgac gcgggcggtg gcagttgacg
     3241 acgctgttcc gcccagaatg ccgcatcacg ctgccaggct tcactttcgc ggtactgctg
     3301 atactcttcc actacatcag cgaaaggcgt aaacggcgaa gcaggcgttg gttcgccacg
     3361 cagcaatgcg cagtaaatat tggcgatctg gcgtgtaatg gccgggaaac tgaagccatc
     3421 gaccagcaaa tggtgataac gctgatacca gtaccagcgg ttatccgcca cctgtatcag
     3481 ctgatgaaag accagtggtt taccactatc gacgcgcaga tcttgttgca aatccgcctg
     3541 cattaatgcc cgcgcagtac cgtgcggatc aatgttggtt cgcaggtcga taatttctgg
     3601 cagttcgaac gtctgcgcat catcgaccca ctgccagact tcgccgttat cttccgtaaa
     3661 acgcatccgc aacgtatctg cttgcgctag tccggcaacc accgcgcggg ccagtagtgg
     3721 cgcatcaacc tctccggtta actcaacgta atgcgccacg ctccaggcgg agggtaaatc
     3781 cgacagtttt tctgccatcc agatgccggg ctgtgcggcg accaaaggta aatgctggct
     3841 cattgtgcct cctgcaactg ggtgaaattc gccggtgtca gagtacgcca gtgggcttcc
     3901 agccactgct ggcaggacgc ctgtgactgc ggctgacaca caacgtccca gcctgccggt
     3961 aaggcgcatt gttgcggcca cagactgaat tgcccctgcg cattgcgcaa aatgtaaaac
     4021 gctccctgtg gatcatcgaa gggattactg aatgccatat tcaactcctg tcatggaaaa
     4081 gtggttgcca gaggtcgatt agcccctgca tcaagccacc gcgccaacaa agcgcatcat
     4141 gtccgccgtc aacctgacgc cagaaaatgg attcttttag ggggtgtaat tgcgcataca
     4201 gcgcctgatt ggcctgcatg atcatcggct cgcgaacacc cgcttccagc acaatgcgca
     4261 gaccttcggc gctaacttca ccagttttta gctgttcaag taacatgccc tcttggtgcc
     4321 cgccccgatg cggccaccag tacgaacctg actggcttaa tacacagcca aagcgttcag
     4381 gccagtgcag tccggcatac agcgcggaaa gcccaccaaa actctgtccg gcgaccacgg
     4441 tgcgatcggc gcgatcgcta aaaggggcaa tagctttcac ctggggtaat aactcttgct
     4501 gtactgccag ccagaaatcc gcattacacg gcagttcgtg ggcgcggtgc gtggtgtcga
     4561 tagcgtcgat caacacatac acggcgggcg gaagttgccg acgatgggtc agcgaagtca
     4621 gcgcaggcca gacgggcata ctctgcgccc aaaattcgcc atcaagcaaa actgccagcg
     4681 ggcgttcttc tgctgttgca tcgccgatgg taaaaatcca tacacggcgt gaatttttca
     4741 accgttcact tttccagata atttctttgg caggcgtttc tggcgcttgc ggacaatccc
     4801 atcccggttg cagaggcgct tgcggcattt cgagtgcaga aacagcgtgc cctcgcccgc
     4861 ctttccagct ttgtggattc agcggatcgg ctatcgcctg gggtaatagt tttcgccagc
     4921 cttcgcgtaa ttcgaggcga tcgggggatg gtgcagaaaa aatgtcatcg cgttcggtag
     4981 gaataaagca gtagctgccg cgccagttgg cattgagttg tgtcgtccac tgccaaacat
     5041 cggttcctgc aattcgctgc atcgaccggg gctggctgtt ctgatggtga tcggtcatgc
     5101 cagtgatata gacccatacg cgctttatcg ccgagtggcc ttcagaacct tgtggatcac
     5161 gccaccaaaa agtgacttca aacatttcgt cattcagacg ttgccattcc gggccatgtt
     5221 tcgactgcca ccagctctca cttcctactt ttaacgccgt cacaccataa ccccatgttt
     5281 actgtgcaat tttttattga ttgcagaaat atattgataa tattattgat aactatttgc
     5341 atttgcaata gcgtaatggc gcgccgtggg aagcgcggac attaattaat cagctgcact
     5401 gcgtgtcttt caggatcaaa ggttttcgcg gtagcgggat gcgtcgtgtt gatgacgacc
     5461 atgcccgaca gttgcaattc gtggcaaaaa tgcaggaata aaacaatgaa caagaagatt
     5521 cattccctgg ccttgttggt caatctgggg atttatgggg tagcacaggc acaagagccg
     5581 accgatactc ctgtttcaca tgacgatact attgttgtta ccgccgccga gcagaacttg
     5641 caggcgcccg gcgtttcgac cattaccgca gatgaaatcc gcaaaaaccc ggttgcccgc
     5701 gatgtatcgg agatcattcg taccatgcct ggcgttaacc tgaccggtaa ttccaccagt
     5761 ggtcagcgtg gtaataaccg ccagattgat attcgcggca tgggtccgga aaacacgctg
     5821 attttgattg acggcaagcc ggtaagcagc cgtaactctg tgcgtcaggg ctggcgtggc
     5881 gagcgcgata cccgtggtga tacctcctgg gtgccgcctg aaatgattga acgtattgaa
     5941 gttctacgtg gtccggcagc tgcgcgttat ggcaacggcg cggcgggcgg cgtggttaac
     6001 atcattacca aaaaaggcag cggtgagtgg cacggctcct gggatgctta tttcaatgcg
     6061 ccagaacata aagaggaagg tgccaccaaa cgcaccaact tcagcctgac cggtccgctg
     6121 ggcgacgaat tcagcttccg cttgtatggc aacctcgaca aaacccaggc tgacgcgtgg
     6181 gatatcaacc agggccatca gtccgcgcgt gccggaacgt atgccacgac gttaccagcc
     6241 gggcgcgaag gggtgatcaa taaagatatt aacggcgtgg tgcgctggga cttcgcgcct
     6301 ctgcaatcac tcgaactgga agcgggctac agccgccagg gtaacctgta tgcgggtgat
     6361 acgcagaaca ccaactctga cgcttacact cgctcgaaat atggcgatga aaccaaccgc
     6421 ctgtatcgcc agaactactc gctgacctgg aacggcggct gggataacgg cgtgaccacc
     6481 agcaactggg tgcagtacga acacacccgt aactcgcgta ttccggaagg tctggcgggc
     6541 ggtaccgaag ggaaatttaa cgaaaaagcg gcacaggatt ttgtcgatat cgatcttgat
     6601 gacgtgatgc tgcacagcga agttaacctg ccgattgatt tcctcgtaaa ccaaacgctg
     6661 acgctgggta cagagtggaa tcagcaacgg atgaaggact tgagttccaa cacccaggca
     6721 ctcaccggaa cgaataccgg cggtgctatt gatggtgtga gtgccaccga ccgtagccca
     6781 tattcaaaag cagaaatttt ctcgctgttt gccgaaaaca atatggagct gactgacagc
     6841 accatcgtaa cgccggggct gcgtttcgat catcacagta ttgtcggcaa taactggagc
     6901 ccggcgctga acatatcgca aggtttaggc gatgacttca cgctgaaaat gggcatcgcc
     6961 cgcgcctata aagcgccgag cctgtaccag actaacccaa actacattct ctacagtaaa
     7021 ggtcagggct gctatgccag cgcgggcggc tgctatctgc aaggtaacga tgacctgaaa
     7081 gcagaaacca gcatcaacaa ggagattggt ctggagttca aacgcgacgg ttggctggcg
     7141 ggcgtgacct ggttccgtaa cgattatcgc aataagattg aagcgggcta tgtagctgta
     7201 gggaaaaacg cagtcggcac cgatctctat cagtgggata acgtgccgaa agcggtggtt
     7261 gaaggtctgg aaggatcgtt aaacgtaccg gttagcgaaa cggtgatgtg gaccaataac
     7321 atcacttata tgctgaagag tgaaaacaaa accacgggcg accgtttgtc gatcatcccg
     7381 gagtatacgt tgaactcaac gctgagctgg caggcacggg aagatttgtc gatgcaaacg
     7441 accttcacct ggtacggcaa acagcagccg aagaagtaca actataaagg tcagccagcg
     7501 gttggaccgg aaaccaaaga aatcagtccg tacagcattg ttggcctgag cgcgacctgg
     7561 gatgtgacca agaatgtcag tctgaccggc ggcgtggaca acctgttcga caaacgtttg
     7621 tggcgtgcgg gtaatgccca gaccacgggc gatctggcag gagccaacta tatcgcaggt
     7681 gccggggcgt atacctataa cgagccggga cgtacgtggt atatgagcat taatactcac
     7741 ttctgaagct aacgtcagat tgttgacaaa gtgcgcgtag ttcatgccgg atgcggcgtg
     7801 aacgccttat ccggcctaca aaaatcttgc caattcaata tattgcagga ccacgtaggc
     7861 ctgataagcg tagcgcatca ggcagttttg cgtttgtcat cattctctaa tatggttgac
     7921 atgaaaacta cgcatacctc cctccccttt gccggacata cgctgcattt tgttgagttc
     7981 gatccggcga gttttcgcga gcaggattta ctctggctgc cgcactacgc acaacttcaa
     8041 cacgctggac gtaaacgtaa aacagagcat ttagccgggc ggatcgctgc aatttatgcg
     8101 ctgcgggaat atggctataa atgtgtgcct gcaatcggcg agctacgcca acctgtgtgg
     8161 cctacggggg tatacggcag cattagtcac tgtgggacta cggcattagc cgtggtatct
     8221 cgtcaaccga ttggcattga tatcgaagag attttctctg tacaaaccgc aagagaattg
     8281 acagacaaca ttattacacc agcagaacac gagcgactcg cagaatgcgg tttaaccttt
     8341 tctctggcgc tgacactggc attttccgcc aaagagagcg catttaaggc atgtgaaaga
     8401 caaactgatg caggatttct gcgctaccaa atcagaaatt gtcagcatca acagatgaat
     8461 ataagcgggc cgaatggcga ccatatcgtg cagtggttag taaggggtaa taatgttatt
     8521 accttgtgcc tggcagataa acacccgcac actgcagcat gcgggttatc tggcgggtaa
     8581 ttcaaagcat ttcccgtgcc tgagctgcaa ttccggcagc atcaaatcca tgatgctgac
     8641 gcaaccaccc acgatccgcc gctgcggcat attctccatc cataatgcct gcgcgtttta
     8701 atgtgatccc cgttccggcc tctgccaaca cttccgcgac caggctacca aggccgccat
     8761 tgacgttatg ctcttcaacc gtcatcactg ctttgcatcc ttttaacgct gccagcaatg
     8821 ctttggtgtc gcatgggcga atcgacggta cgctgacaac tgtagccgcg ataccagact
     8881 gcgctaaaag ttctgcggca tcgaccactt catgaacggt agagcctgtg gctaccagcg
     8941 ccagttcgct gccctcgcgc aaagtcacca cggcacctgg tacaaatcgg tagtcctcat
     9001 cgtggatttc cggcagggct ttaccgtcca tacggatata aacaggcccc tgatagcgaa
     9061 gagcataatc aatcacctga cggcactccc gcggtgaaga cggggcaaag atttgaatat
     9121 tgccgaaacc acgcataacc gcaatgtcat caatcgcatg atgcgtgctc gccagcggtc
     9181 cataactcgc cccggcattc agaccgaaca attttacgtt ggtaaagttg tagcagacat
     9241 caaccttgat ttgctcgtta gcgcgagcga taagaaacgg cgcggcattg caggtaacag
     9301 ccaccttgcc gcccagcgcc agcccggcag ctgtaccaac catgctctgt tcggcaatac
     9361 ccacattcac cagtcggtca gggaattgct taataaaggg agcaattttt gccgtagacg
     9421 ttgaatcagc caccacaggc accaaatcga tcccctcatt tacggcatcg ataaacgcct
     9481 gaaccatcac atttgccaga tgttcactgt tacgcatctt tgagttcctc cagtgcctgt
     9541 tcaatttcat cgcctttcgg cacccggtga tgccattccg cacgcccttg tataaaggaa
     9601 atgcctgccc ctttagtggt gtgggcaatc accacattcg gtttgccttc ctgtttaagc
     9661 tcttcaagcg tcgcgacaac cgaagccata tcattgccct gacattcagt aacctgcata
     9721 ccgaatgcca gccatttctc tgccagagga tcggtgttca tgatttcgcg cgttggcccg
     9781 gctaattgca gattattttt gtcattaata atgaccaggt tatcaagccc gtaatgcgcc
     9841 gccgccagtg cagcttccca gttactgccc tcagccagct caccatcgcc agtaattaag
     9901 aagatacgac gacggctttg actttttttc gccgccagcg caagtcctac agccacaggg
     9961 aaaccatgcc ccagtgctcc ggtattcagt tcaatacctg gcgttttttg ccgaaccgga
    10021 tggccgggta agtgtgaatt tggctgctga taagtggaaa gccattccag tgggaagaat
    10081 cctgcttccg ccagcacgca gtagtaacat ccaacagcat ggccttttga ctgaatgtat
    10141 atatcccggt catcgtcgtg aaggcgatcc ggtgcgacat tgagaatacg aaaatagagt
    10201 gcggtcagta attcaacctg agagagatcg gccccggtgt gtccacctgc cgggctattg
    10261 gcatttaagc gcacgatatg gcggcgtacc gcccgcgctt tacgctcaag ggtctggata
    10321 tcatagctat agggattcat ttaatacctc tgaatttatt ttcaacatgg aatatttatt
    10381 ccaccgggga ctaaaaaaac cagaaatccc agggcgttgc gcggcaagac ttacgctgct
    10441 gcatcatact caggaacatt ctgcctcctt acaccgtcag ttacatgtcc gggtaatcat
    10501 cagttgacct gatatcatct tgaatatata ttcaataatg attctttatt caattatgcc
    10561 gcctgttttt tatttttgtc catcgctgat tttaaggatt ggttaaagtt agaactccat
    10621 cacacttaac tgtcttcttt tttcgcgtat gataaaagcc tctaagataa gggcggtacg
    10681 ttaatgattt acggggaaat atatggctaa gcaggatgaa cagcggctac tggtgaagat
    10741 cgccactctt tactatcttg aggggcgaaa acagtctgac atcgcccaac ttctctcgct
    10801 ttctcagtca tttatatcca gagcgctaac ccgttgccag aaagaaggcg tggttaaaat
    10861 cagcgttgtc cagccgtcaa atatctttct caatctggaa aaaggcattg aagagcgtta
    10921 cggcattaaa caagcgattg ttgtcgatac cgaagatgat gccagtgatc acaccattaa
    10981 acgcgccatc ggctctgccg ccgcgcacta tctggaaacg cgtttaaggc caaaagattt
    11041 cattggcgtc tcttcgtgga gttctacgat tcgcgcgatg gtcgatgaag ttcacgccca
    11101 aaacttaaaa gccagcggcg ttattcagct gctgggcggc gtggggccaa acggcaatgt
    11161 gcaggcaact attttgacac agaccctggc ccagcatctg aattgcgaag cctggttgct
    11221 tccttctcaa agcattgaag gttcggtaga agagaaaaaa cgcctggtgg caagccagga
    11281 tgtggctgac gttatcgcca gatttgacga cgtcgatatc gccattgtcg gcatcggtat
    11341 ccttgaaccc tcgcagttgc taaaaacctc gggcaactat tatcacgagg atatgttaca
    11401 ggtactagcg gatcgcggtg ctgtgggcga tatctgcctg cactactacg ataaacatgg
    11461 tcagcccgtg ttgcaggacg atgaagatcc agtgattggt atggcactgg ataaaatcaa
    11521 aaaatgcccg aatgtggtgg cactggcggg cggcaaagac aaagttgccg ccatcaaagg
    11581 cgccatgcag ggtggttata ttgatgtttt gatcactgat ttccccaccg ccagaatgct
    11641 gattgcggat taatcctctc ctccctttct tctgcccgcc tttgcgaaaa aatcttctcg
    11701 caaaggtgat tgcttaaaaa gcctttaata tcaataaact gaaaggattc aacaatcctg
    11761 tttctcgagg ctttctcacc ctgtcaccac gcttctaagt gtgatagcca gcacatttga
    11821 tgattttttc tctagctcag gaagaataat ctggctaagg tataaataaa taatcagtat
    11881 cgaatatata ttcatgcctt aacacaaaat gtcacagcag ggcctgatga agtaataacg
    11941 tgaccatgag gaacgcctca tctccttcca cgccaggatg caatgtgact caggagacta
    12001 cctatgtttg caggaaataa agagttcgtt attgcgttgg atgagggtac caccaatgca
    12061 aaagccatcg cacttgatgg tttcggtaac gtggtggcga agttttctca accactggct
    12121 attcagacgc cacgtgaagg ctgggttgag caatccgggg aagcgttaat ctctgcctcg
    12181 cttgatgtta ttgcccaggc catcaaccac gtgggggcag aaaatgtagc ggctctggct
    12241 atcagtaatc agcgtgaaac ggctatcggc tggtatcgtc agagtggaaa accactcaat
    12301 gcggcgatta cctggcaatg cacgcgcagt gccgagtttt gtgaaacact gcgtcgcgag
    12361 ggcaaagagc agcaaattaa aacgactacg ggtcttccta tcgccccgct attttccgcg
    12421 tccaagatgc gttggctact ggaatctaca cctgagggtg tgcaacttgc cgaccagggt
    12481 gaaatttgtc tcggcaccat tgatgcctgg ttgctatgga acttgactgc tggcgattca
    12541 ttttgctgcg actactctaa cgccgcgcga actcaattac ttaacctgaa aacaggcgag
    12601 tgggatgagg aaatgctggc gttgtttcac attccacgcg ctgcattgcc agaaattaaa
    12661 ccttccagtg gcctgttcgg ttatacccga gggcttaaaa caattccgga tggtattccc
    12721 gtcatggcga tggtgggcga ttctcacgcc gctctttttg gtcatgcgtt gggagaagcg
    12781 ggatgtgtga aagcgaccta tgggaccggt tcttccgtca tggcccccgt caactctgct
    12841 cagtgtgata tcgccacact tgccacaacc attgcctggc atgatggcga aaatattatt
    12901 tacggtctgg aaggaaatat cccccacacc ggcgatgccg tggcatggat ggctgacagc
    12961 accggtttaa gtgaactgac agacgcggag ctggccctcg aactgaatac cctgccagcc
    13021 tcagtggatt cgactatggg tgtttatttt gttcctgctc tcactggcct gggcgcgcca
    13081 tggtgggatg acagcgctcg cggcgtggtg tgtggcttaa gccgtggcgt aaagcgcgcg
    13141 catttgattc gtgccgcact ggaatcaatc acttatcaaa ttgcagatgt agtggaggca
    13201 atgcaacagc atgatgattt tcacttatcg gcgttaatgg tggatggcgg cccgaccaaa
    13261 aatgactggt tgatgcaata tcaggcggat ctgctgggtt gcccggtgat gtgcagtgat
    13321 gtgccagagc tttcagccat tggtgcgggc ctgctcgccc gcaaagcgct tatccccggt
    13381 accgttgcag atttaaaagc gttattaact gaacacagtg aatttaaacc ggacatggcg
    13441 cgtcatcagc gactgcaaaa gagatggcag gagtggcgtc atgcggtaaa cagaacatta
    13501 tggaaaccgg catcgtcaat ttgacgaaca agactaacca acgtaaggag aataccggcc
    13561 cataaataat tcaggcttta cgttgtttca ccgtacccga caataacaac acagagagaa
    13621 cattgtcatg aaattacgtt taacactgtt aaccgctgcg accctgactg ctctttcttt
    13681 ttctgctcat gcggtagaaa aaggaaccat tatgattatg gtcaattcac tggataatcc
    13741 ttattacgcc tcggaagcga aaggtgccag tgaaaaagcc cagcaactgg gatataaaac
    13801 gaccattctt tctcatggtg aagatgtcaa aaaacagaat gaactgatcg atactgcaat
    13861 tggtaaaaaa gttcagggca ttattttaga taatgccgac tccactgcca gtgttgcagc
    13921 cattgaaaaa gcaaaaaaag caggtattcc cgttatatta attaatcgcg aaatacctgt
    13981 ggatgatgtt gcactggtgc aaattaccca taataacttc caggctggct cggaagttgc
    14041 caacgcattt gtcgagaaaa tgggagagaa aggaaaatat gccgaactca cctgtaatct
    14101 tgctgataat aattgcgtaa ctcgctcgaa atctttccac caggttattg accagttccc
    14161 tgaaatggtc agcgtggcga agcaagatgc caaaggcacg ttaattgatg gcaaacgaat
    14221 aatggacagt attctgcaag cccatcccga tgttaaaggg gtgatttgtg gtaatggtcc
    14281 ggttgcactg ggagctatcg cagcactgaa agccgccaat cgcaatgacg ttattgttgt
    14341 cggcattgat ggcagtaacg atgagcgcga tgccgtgaaa gccgaatcat tgcaggctac
    14401 cgtgatgtta caagcacaag ccattgccgc gcagggtgtt accgatctgg ataattacct
    14461 gcaaaaagga gttaaaccag ccaaacagcg ggtgatgttc cgcggcattc tgataaccaa
    14521 agataacgca gataaagtcc aggatttcaa tatcaaatcc tgatcccagg tcgttgcgcc
    14581 ctcacaatga gggcgcagag gagaatatta tggataaaaa agtctggctg gcgctggcaa
    14641 ttctgagcct gggtggatgt cgcatcgtct cgcagcagga acttgccgat ctcaaatccc
    14701 cgcctaatcc tcatatggca aatatcgatc agacctggca gaaaaacatc gtgccgcagg
    14761 tggtagaaaa tgcgcgtccg gttgcggagt taatggcggc gctacaggca gaaaaagatg
    14821 tcgatgctgc ctgtaaaacg ctgggctatc gttctcagga agaaaacccg tgtattttct
    14881 acgttaaggt cgaaggtagc atcaccaata ttgatgcgaa atcccgtagc ggaaaaatga
    14941 ccatcacaga tatcagcggc acaaatattg tcgtgcagac ggggccaact cttcgcggca
    15001 cgcttctgcg agatgcatat aaaggtgcaa gctatgagca ttttaacgat caggttttat
    15061 ttggtgaata tggtcgtgct atcaatgagc aagccatcaa tatgattcag tcggcaaagc
    15121 ttaaaaccgg ggaaacggtt caggtttatg gcgtattttc tgcatgggat attccgcaaa
    15181 ctatcccgga tataacgcct gcgaaaatag ccagggcggg aggcgagtaa tatgttgccg
    15241 caagaaattg ccccccccca gactgatgaa tcacctgtaa tcatagaaac gcatgactta
    15301 tcgcgtattt atccgggtgt ggtggcgtta gataacgtga attaccgagt ctatcgcaat
    15361 aaagtaaatg tgcttattgg tgaaaacggt gcgggtaaat ccacaatgat gaaaatgctg
    15421 gcgggggttg aaacaccttc ctcaggcaag atcattcttg atggtaaaga agtcacccta
    15481 aattctaccc atcaggccga aaaacatgga attagtatca tttttcagga gctgaattta
    15541 ttcccgaata tgaatgtcat ggataatatt ttcatggcga atgaattttt tcagaaaggg
    15601 aagattaacg aaaaatatca atatgaatta gccaaatctc tattagaaag actggaactg
    15661 aatgttgatc cctataccca actgggagag ctaggtattg gccatcagca actggtggag
    15721 attgcccgtg ccctttctaa agatacccgg gtactgatta tggatgaacc gacatcggcc
    15781 ctcagccagt cggaagtaaa agtccttttc aatgtgatcg cacaactgaa gcgccgtggt
    15841 gtcaccatca tttatatctc ccatcgtctg gaagagttga tggagattgg cgatttcatc
    15901 accattttcc gtgatggtcg ctttattagt gaacgcgccg tgagcgatgc cagtattcca
    15961 tggatcatcg agcaaatggt cggtgataag aaaaaacatt tcgattatca accagctaca
    16021 caaggtgaca ctgtactggc ggtacaaggg ttaacggcgc tgcatcccag cggtggttac
    16081 aaactcaatg atgtgacatt cagcctgcat aaaggcgaag ttatcggcat ttacggcttg
    16141 ctgggcgcag gacgtactga actatttaag ggattggtgg gcctgatgcc ctgtcagagt
    16201 gggaagatcc agcttaatgg tgaaaccatc gacaaatgcc atttccaggc gcggctcaaa
    16261 aaggggctgg cgttggtgcc agaagatcgc cagggcgaag gcgtcgttca gatgatgtcg
    16321 attcagtcca atatgacgct atccgatttc agcctgcaag gttttcgccg tgcctggaaa
    16381 tggctgaatc cgcaaaaaga gagcgccagt gtgaaagaga tgatcctgca actggcgatc
    16441 aaagtcagcg atgccgaact ccctattacc tcactcagcg gcggtaacca gcaaaaagtg
    16501 gtacttggca aagcgttgat gacccagcca caggtggtgt ttcttgatga acccacacgc
    16561 ggcattgatg ttggcgcaaa aacagatgtc tatcagctgg tgggaaaaat ggcacaacag
    16621 gggctggcag tcatgttctc ttcttcagaa ctcgatgaag tcatggcact ggcagatcgc
    16681 attctggtga tggctgatgg ccgtattacc gctgatctcc cccggggcga agtcacccga
    16741 gagaaactga ttgcggcttc aacacctcaa gattaatcag gctggaattt cttatgaatc
    16801 agaaatatat gatctatatg tacctgttga aggccagaac gtttatcgct ctgctattag
    16861 tgatcgcctt ctttagcgtg atggtgccta acttcctgac agcctctaac ctgttgatca
    16921 tgactcaaca cgttgcgatt accgggctgc tggcaatcgg aatgacgctg gtgatcctga
    16981 ccggcggcat cgatctttcc gtcggcgcgg tagcggggat ctgcggaatg gttgccgggg
    17041 ctcttctaac aaacggcctg ccattgtgga gcggcaatat tattttcttc aacgtgccgg
    17101 aagtgatcct ctgcgtggcg gtatttggca ttttagtggg actggtgaat ggcgcggtaa
    17161 tcacccgctt tggtgtcgcg ccctttatct gcaccctcgg tatgatgtac gtcgctcgtg
    17221 gctctgccct gctgtttaat gacggcagca cttatcccaa tctcaacggc atggaaacgc
    17281 tgggcaacac gggtttcgcc acccttggtt ctggcacgtt tttaggcatt tatctaccaa
    17341 tctggctgat gattggcttt ttgattttag gctactggat cacgactaaa acgccgctcg
    17401 gtcgttatat ctatgccatt ggtggcaatg aatctgccgc gcgactggca ggcgtcccca
    17461 tcgtaaaagt taaagtgttc gtctatgcgt tctccgggtt atgcgccgca tttgtcggtt
    17521 tgattgttgc ctcgcagctg caaactgctc acccaatgac cggcaatatg ttcgaaatgg
    17581 atgctattgg cgccacggtt cttggtggaa ctgcactggc gggtggtcgg ggacgtgtct
    17641 ctggctcgat tatcggtgcc tttgtcattg tcttcctcgc cgatggcatg gtgatgatgg
    17701 gtgtcagtga cttctggcaa atggtcatta aaggcctcgt tatcgtcacc gccgtggtgg
    17761 tcgatcagtt ccagcaaaaa ttgcaaaaca aagtcatttt gatgcgtcgc catgaagaaa
    17821 agatggccgc cgcacctgcc gtaaaaccgc tttccagtta attaacatca ttccaccggg
    17881 cattaaattg cccggcttat acaggggaaa aaatatgcaa cgtaattttc agggtaagac
    17941 tgtagttatt accggggcat gccggggaat tggggccggg atcgctgaac gcttcgcccg
    18001 cgatggcgca aatctggtga tggtttctaa cgctgaacgc gtacatgaaa ctgcagagca
    18061 actgcgccaa tgttatcagg ctgacatttt gtcattagaa gtcgatgtga cagatgaagc
    18121 gcaagtacag gcactctatg aacaagcggc tgcacgtttt ggcagcatcg atgtttccat
    18181 tcagaatgca ggcattatca ccatcgatta ttttgatcga atgccggctg ctgatttcga
    18241 aaaagtgctg gcggtgaaca ctacggcagt ctggctctgc tgccgggaag cagccaaata
    18301 tatggtgaaa cagaatcacg gctgcctgat caacacctct tctggccagg gacgtcaggg
    18361 gtttatctat accccacact atgcagccag caaaatgggt gtgatcggca ttacgcaaag
    18421 cctggtgcac gagttagcgc agtggaatat cactgttaac gccttctgcc cgggtattat
    18481 cgaaagcgaa atgtgggact acaacgaccg cgtatgggga gaaattctca gtaccgatga
    18541 caagcgttat ggcaaaggcg agcttatggc ggaatgggtg gaaggtattc cgatgaagcg
    18601 tgccggtaaa cctgaagatg ttgccggact ggttgccttc ctcgcctctg acgatgcgcg
    18661 ttatctcacc ggtcaaacca tcaacatcga cggcggcctg attatgtcgt aatgcccctg
    18721 cgggctacta agagagatat caaaatgatc gcaaaagatt ttgctcaaca attgtttgat
    18781 ctccacgatc gcgtcgcctt tgtcactggc gcaggtagcg gtatcggaca aatgatcgct
    18841 ctggctttag ccagcgcagg cgcacgagtg gtgtgttttg atctacggga agatggcgga
    18901 ctcaaagaga cggttaatca tatcgaagca cttggcggac aagccttgta ttacacgggc
    18961 gatgttcggg aattgagaga tcttcgcgca gcagtcgctc tggcaaaaac acattttggt
    19021 cgtctggata ttgccgtaaa cgccgctgga atcgctaacg caaacccggc gctggagatg
    19081 gaaagcgagc aatggcaacg cgttatcgat atcaacctga ctggtgtgtg gaattcctgt
    19141 aaggcggaag ctgagttaat ggtagagaac ggagacggtt cgattataaa tattgcgtca
    19201 atgtccggga ttattgtcaa tcgtggtctg gaacaggccc actacaacag ttccaaagcc
    19261 ggcgtgattc atttatctaa aagtcttgcg atggaatggg tgagtaaggg gatccgcgtg
    19321 aactcaatca gcccaggata taccgcgaca ccaatgaata cacgacctga aatggtacat
    19381 cagactcggg agtttgaaag ccagacgccg attcaacgga tggcgaaagt ggaagagatg
    19441 gcgggtccag cgctgttcct tgccagcgat gcggcgtcgt tctgcacggg tgtggatctg
    19501 gtggtcgatg gtggctttgt ctgctggtaa aaatggtttc gcccgcagcc atcactgacc
    19561 gcgggcgaaa gtgtaaagca ggtgccttac caccctgacc tgacaaccgg atatgcgggg
    19621 attgctcccc gcacaacggc tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa
    19681 tattacgttc ctttacggta aactcacaca gcgagtctcc gaccagaagc gtaaatccca
    19741 gcaccgtcaa acacagcact atgactgcca caagggcata tttcgtcagc atgtttatat
    19801 tgcctccatg cgaagaaggg actaccatcc aaattgtctg gtttggagtg gcagccccag
    19861 ggttgataga aataactcct ggggcttcat ctttccagac ctcaaagtta acctgaaacc
    19921 ggaaagcttc aggcacccgc cgttatctta cccttcctca acaaaatagc tgtttattta
    19981 tacagtaagt cctgtttcgg acttctgttg gcaaaactga tcattgccct taaaacgaca
    20041 tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accaccctga
    20101 cctgacaacc ggatatgcgg ggattactcc ccgcacaacg gctacttctt cggttcgtaa
    20161 gcgagaacag ccttaaactc aatattacgt tccttcaccg taaactcaca cagcgagtct
    20221 ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc cgcaagggca
    20281 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccaaattgtt
    20341 caggtttggg taggtagccc cagggttgat agaaatatcg tcctggggct tcatctttcc
    20401 agacctcaga gtaaacctga aaccggaaag cttcaggcac ccgcagttat cttacccttc
    20461 ctcaacaaaa tagctgttta tttatacagt aaatcctgtt tcggacttct cactccccat
    20521 ttaatcaact gaagctgaac ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc
    20581 cgccagccag ctctgccata acttgtcaca ttgcaaaatg tagccaattt gcttcagtca
    20641 ccatcaccaa aaaaagaaac attccagaac ttccgcatct cgcccagccg ctcccacttc
    20701 tatactgaat agaaaacgcc aacataagag aaacctatgc cattacccga ttttcatgtt
    20761 tctgaacctt ttaccctcgg tattgaactg gaaatgcagg tggttaatcc gccgggctat
    20821 gacttaagcc aggactcatc aatgctgatt gacacggtta aaaatcagat cacggccgga
    20881 gaggtaaagc acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat
    20941 atcaaccagg ctgccgggca attttcagcg atgcagaaag tcgtattgca ggcagcggca
    21001 gaccatcatc tggaaatttg cggcggtggc acgcatccgt ttcagaaatg gcagcgtcag
    21061 gaggtatgcg ataacgaacg ctatcaacga acgctggaaa actttggcta tctcatccag
    21121 caggcgaccg tttttggtca gcatgtccat gttggctgcg ccagtggcga tgacgccatt
    21181 tatttgctgc acggcttgtc acggtttgtg ccgcacttta tctccctttc cgccgcgtcg
    21241 ccatatatgc aaggaacgga tacgcgtttt gcctcttcac gaccgaatat tttttccgcc
    21301 tttcccgata atggcccgat gcagtgggtc agtaactggc aacaatttga agccctgttt
    21361 cgctgcctga gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc
    21421 cccagccccc attttggtac ggtggaagtt cgggtgatgg ataccccgtt aacccttagc
    21481 cacgcggtaa atatggcggg attaattcag gctaccgccc actggttact gacggaacgc
    21541 ccgtttaaac atcaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgt
    21601 cgctatgggc tggaaggtgt cattaccgat ccgcatactg gcgatcgtcg gtcactaacg
    21661 gaagccacct tgcgtttact ggaaaaaatc gccccttctg cacataaaat tggcgcatcg
    21721 agcgcaattg aagcactgca ccgtcaggtc gtcagtggtc tgaatgaagc gcagctaatg
    21781 cgtgatttcg tcgccgatgg tggctcgcta attgggctgg tgaaaaagca ttgtgagatc
    21841 tgggccggag aataagtcgg gaattgcctt acagaagaat atccctgaca atggtgtttt
    21901 aacttacatt taacaatgtt atgaaacacc ctttagaaac cttgaccacc gcagcaggca
    21961 ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc
    22021 tggcacaaaa actggtgacc acgttccatc tgatggatct tagccagctt tacacgttat
    22081 tgttttgtct gtggttttta gtgctgggcg ctattgagta ttttgttcta cgctttatct
    22141 ggcgacgctg gttctcgctg gcggattaaa cgtcgattat cgccgaactt cgcggcatac
    22201 tttgcttatt ctccttcgcc taaaggaaca tttatggata agcaatcact gcacgaaacg
    22261 gcgaaacgcc tggctcttga gttacccttt gtcgagcttt gctggccttt tggcccggag
    22321 ttcgatgttt ttaaaattgg cggcaagatt tttatgctgt cgtcggagct gcgcggcgtc
    22381 ccctttatta atctgaagtc cgatccacaa aaatccctgc taaatcagca aatataccca
    22441 agcatcaagc cagggtatca catgaataaa aagcactgga tttcggtgta tcccggcgag
    22501 gaaatctccg aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat
    22561 ggtctggcta aacgcgatca aaaacgactg cgtcctgact caaccgtaaa tcaatagcgc
    22621 atttttctcg cttaccattt catctcgatc cttgtaatct gctggcacgc aaaattactt
    22681 tcacatggag tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca
    22741 tttgatgcca gcaaaaaact taccccggaa caggccgagc agatcaaaac tctactgcaa
    22801 tacagcccat ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa
    22861 ggtaaagcgc gtgttgccaa atccgctgcc gataattacg tgttcaacga gcgtaaaatg
    22921 cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg
    22981 aagctggttg ttgaccagga agatgccgat ggccgctttg ccacgccgga agcgaaagcc
    23041 gcgaacgata aaggtcgcaa gttcttcgcc gatatgcacc gtaaagatct gcatgatgat
    23101 gcagagtgga tggcaaaaca ggtttatctc aacgtcggta actttctgct gggcgtggcg
    23161 gcgctgggtc tggacgcggt gcccatcgaa ggttttgacg ccgccatcct cgatgaagaa
    23221 tttggtctga aagagaaagg ctacaccagt ctggtggttg ttccggtggg tcatcacagc
    23281 gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc cgcaaaacat taccttaacc
    23341 gaagtgtaat tctcccttgc cgggcatctg cccggctatt tcccctcaga ttctcctgat
    23401 ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt gtataaagga gacgttatgc
    23461 aggatttaat atcccaggtt gaagatttag cgggtattga gattgatcac accacctcga
    23521 tggtgatgat tttcggtatt atttttctga ccgccgtcgt ggtgcatatt tttttgcact
    23581 gggtggtact gcggaccttc gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa
    23641 tcattaccca gaataaactc ttccaccgtt tagcttttac cctgcagggg attatcgtca
    23701 atattcaggc ggtattctgg ctacaaaaag gcaccgaagc ggcagatatt ctgactacct
    23761 gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt cttctcgttg ctggatgtga
    23821 ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt accgctgaaa gggatttttc
    23881 aggggattaa actgatcggc gcgattctgg tcggcatttt gatgatctcg ctgctgattg
    23941 gtcagtcgcc agcgattctg atcagtggtc ttggtgcaat ggctgccgtg ctaatgttgg
    24001 tatttaaaga tccgattctt ggtctggtgg caggtattca gctttccgcg aacgatatgc
    24061 tgaaactggg tgactggctg gagatgccga aatacggcgc ggatggcgcg gtgatcgata
    24121 ttgggttaac caccgtcaaa gtgcgtaact gggacaatac cattaccact attcccacct
    24181 ggtctctggt ttctgactcc tttaaaaact ggagcgggat gtcagcttct ggcgggcgac
    24241 gtattaaacg cagtatcagt attgatgtca ccagtattcg ttttcttgat gaagacgaaa
    24301 tgcaacgcct gaataaagcg catttgttaa agccttattt aaccagccgc catcaggaaa
    24361 ttaatgagtg gaatcgccag caaggttcta cggagtcggt attaaacctg cgccgaatga
    24421 ccaatattgg aacctttcgt gcctatctga acgaatatct gcggaatcat ccgcggattc
    24481 gcaaagatat gaccttaatg gtacgccaac tggctcctgg tgataacggt ttaccgcttg
    24541 agatctatgc gtttaccaac accgtggtgt ggctggaata tgaaagcatt caggctgata
    24601 tattcgatca catatttgcg attgtcgaag agtttggtct gcgacttcat cagtcgccaa
    24661 ccggcaatga tattcgctct ctggcgggtg catttaagca gtaattaaaa aaaccgctct
    24721 catcgaatgg atgagagcgg ctttcagatt gttgacatca ttttgtcgga tgcggcgtga
    24781 acgccttatc cgacctacag aatcgttaca aattgtaggc ccgataagct tgcgcatcgg
    24841 gcaaatcagc ctcacatcag cttaattcac tttcctacgc aatgttttaa acgccacaaa
    24901 caggaacaca atccacaccg gcagcagaat cgctgacaag cgcatatcat ccatcgtgca
    24961 catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag
    25021 cagcgcctta aattgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg
    25081 cgccaggcag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    25141 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    25201 ggaaagcatc agcgagttaa tcggcacgcc gcgacggctg acgcgagtca aaaactttgg
    25261 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    25321 atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    25381 gtcgagatta tggaaaatca tcacaaacgg gctactattg gatttcactt ccacccacgg
    25441 gtagagcgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc gatacaccac
    25501 ctgattcact gctttaggga tgcttttttc cggatcgcgc gcttcagccg cagtaatccc
    25561 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagtccatt
    25621 ccagccggtg gcaaagaaac cgccgtagcg ccagaggtta tcgatactgg ctttctcacc
    25681 gccgtgccca gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac
    25741 tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    25801 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    25861 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    25921 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    25981 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccgagctggc gcataatcag
    26041 gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat
    26101 cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    26161 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    26221 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    26281 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    26341 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    26401 ataaacagcg acagcaaagg cgcggtgatc atgccgccaa tcatcggcgc ggcaatccgg
    26461 ctcatcacct ctgaccctgc tcctgttccc cacagaatcg gcagcagacc agcgataatc
    26521 accgccaccg tcatcgcttt tgggcgcacg cgcagtaccg cgccgtgata taacgcctca
    26581 tccagcttct gctcgctgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    26641 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    26701 aaaccggtgc ccgtcgccac ggaaagatga aagcccatcc accacagcaa ccagatgccg
    26761 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaac
    26821 gccagataca acagcacgaa gatgatcatc agcgtcatcg gcaccatcag tttcagctta
    26881 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt acccggtttt
    26941 aactgcactt tttcagctat cgctttttgc agatcgtgaa ccaccgacac catgtcacga
    27001 tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    27061 ggtccggtag agactttaac gtcggccacg tccgccagag tgatttgctg cttcatcggc
    27121 gtgaggatcg gcaactggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    27181 agattaattg gataacgagc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    27241 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    27301 cggttaatct caacgttgat ataacgccca ccttccagac gctcagcaag cgcagaagcc
    27361 acgcctggca ccgttcgcgc cacttcttca atttgctccg ccatcgtgtc gatatccgcc
    27421 agcacagtgc cggaaacttt aatgccgata gggcttttaa tgccagtcga gagcatatcg
    27481 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg cacggtgtta
    27541 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    27601 agctggatag tcgtttctac catctccagt ggagctgaat cggtggcggt ttccgctttc
    27661 ccggttttgc caaatacccg cgccacttcc ggtacgctca taatcagctt gtcgtttttt
    27721 tgcagcatgc tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    27781 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    27841 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    27901 agcaacagcg gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    27961 ggaattttgc cacggatcca gtagcccatc aggatcggga tcactacgat cgccagtagc
    28021 gccgcgcccg ccatcgcata ggttttggtg aacgccagcg ggccaaacag acgaccttcc
    28081 tgcccttcca gggtgaagat cgggataaac gacagcgtga taatcagcag actgataaac
    28141 agcgccggcc ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    28201 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc attctcgatc
    28261 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc caacgacata
    28321 atattggcgt tcagtccctg aaagtgcatg acaataaaag caatacacaa ccccagcggc
    28381 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    28441 gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcaatggc gcggtcaatg
    28501 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact gcttttcagc
    28561 gtttccagtt tgtccttcac agcggcgatc acttcacggg cgtttttgcc ggaacgcagg
    28621 atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt cggcaatgcc ccggcgcatc
    28681 tccgggccaa cctggacctt cgcaacatca cgcagataaa cgggcacgcc attttcactg
    28741 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    28801 atatattccg cttccgccag ttctatcgac gaaccgcccg cttcctggtt tgaagcatcc
    28861 agcgcgcttt ttacgtcggc gagactgatg ccatactgcg ccaggcgctg aggatcgcta
    28921 accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac gtcagggatg
    28981 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    29041 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggc
    29101 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    29161 acccgcgagc gcgcccagta cggatcggtg ccatcttcga aaatgacata cacataagag
    29221 tcgccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    29281 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    29341 ttaataatca cctgcacatc ggaaagatcc ggcagtgcat ccactggcgt gttaatgatg
    29401 gtccaggtgc cccagatact cagaaacaac gcgcccatca gcaccagaaa acggttcgcc
    29461 accgagcgac gaataatcca ttcaatcatt ggctattccc tcaatgcgca tgggtagcac
    29521 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcagaatca atcaggaaca
    29581 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaacgcg gtgacgcctt
    29641 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg cccgtcagca tcaacggtaa
    29701 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    29761 cgctggcggt attgagttgc aacccggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    29821 tgtcgatttc cagacgcaac tgcagcgtgc gggtcgcggc atccacgccg ggtagcaggg
    29881 tccatttgcg aatggtgagt gttttatcag gtcgcgccgg aacggtgagc gtaaactgcg
    29941 aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc cacaccgggt
    30001 ccataccctg aattttcgcg accacgttat ctttggcgat attcattccc gcgcgcagat
    30061 caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt
    30121 gcgtggcgat caggcggcga atatcagcct ccggcattcc cgccagtcgc agtcgctcaa
    30181 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    30241 gcgcttccac ccagtcggga atggtcaggt cgagaagcgg cgcgcccttt tgcactttat
    30301 cgcccacggt aagcggatac accttgtcga taaacccggc ggcgcgggcc tgcacaatgg
    30361 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggaaaaa gtcagcggcc
    30421 cgcgcgtgac ggtcgccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    30481 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    30541 gcgatttccc tggtttatcg aaccgcgtat tgggatacat cgggtcgtac cagaataaga
    30601 ctttacgttc tgcggtcgac gttttttctg cgggcggttc ctcctttgca aaccaggtaa
    30661 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    30721 tcatctcatt aaacccggat tactggctga ctttaatatc ctgtaataaa gaaagattgc
    30781 cctgctggac aaaattaaac gctactttat cgccggtttt aatttcgctc attttcgtct
    30841 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    30901 gatggatggt gatttttttg ctttccagat caacaccttt taccacgcca gtggcgctaa
    30961 taacctgtgg ttgtgcttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    31021 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    31081 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtatata aagaaatttc
    31141 gttaacctga cgggcataat tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg
    31201 ttcagcatcc agcacttcca gataactgac tgcgccgtgc tgatataatg cccgcgcccg
    31261 ttgcagggta atttgcagcg acgcaagata acgctgctgg gcgctgattt gatcgttcag
    31321 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    31381 ataattcacc accgactgct gctggcgaat ttcggtgata tccagattgg cctggttgcg
    31441 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctggc
    31501 gttaaataat gacgacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    31561 cgggaaaaat gccgcacgcg cagcaccaat attggcatta gccgccatta acgcgtgttc
    31621 agcttccata atatcagggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    31681 aacgctttgc aggctgtcgc tgtttactgt ctgcgcttgc ggcagcttgc cgtagcttcc
    31741 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    31801 gctgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    31861 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    31921 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    31981 aaccaacaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttggcgctc
    32041 ggcttcgctc atgttcttta agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc
    32101 agtcgataac tcccgcgtcg tggctgaatc gcctttaaga ttgccgctcc agctgccgct
    32161 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgcgc
    32221 ttcctgcact ttcagcgccg ccatgcgcaa atcccggtta ttcaccaacg cctcgctaat
    32281 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccgcgttct ggtagttatc
    32341 tgctgcgtta accaggccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgg
    32401 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    32461 aagtttacaa ggagacatag gctcataatt tctggtgatt ttatgctgcc aactttactc
    32521 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    32581 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaatatgaaa
    32641 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    32701 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    32761 ggtgattatg atctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    32821 gtgcgcatgt tgcgttccgc caataagggg atgccgattc tgttgcttac cgcgcttggc
    32881 accattgaac atcgcgtcaa ggggctggag ttgggggcgg atgactatct ggtgaagccg
    32941 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctac tgcggcgcgg agcggcggta
    33001 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    33061 acccgcagcg gcacgcgcat cactctgacc agtaaagaat ttactctgct ggagtttttc
    33121 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt atgggacatg
    33181 aattttgaca gcgataccaa cgccattgat gtggcggtga agcggctgcg cggcaaaatc
    33241 gacaacgact ttgagccaaa gttgattcag accgtgcgcg gcgtgggtta catgctagag
    33301 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gcctgacctt
    33361 ttttatcagc ctcgccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    33421 ggtaaaagtg cattttgccg agcaggatat taatgattta aaggagatta gcgccaccct
    33481 tgaacgggta cttaatcacc ctgacgaaac gcaagcccga cgcttaatga cgctggaaga
    33541 tatcgtcagt ggttattcca atgtgttgat ctccctggca aatagccacg gtaaaacggt
    33601 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    33661 agacgcgcag gctggcgagg tgtatctcct ttccggcccg acgatgatga tgccagggca
    33721 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgct
    33781 ggttgacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    33841 ttacatcaat gatttgatga ataaacttat tatgaccgca tcggtaatca gcatcctgat
    33901 cgtctttatc gtgctgttgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    33961 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc
    34021 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    34081 ctttacccga cagtccaatt tctcagcgga tatcgcccac gaaattcgca cacctattac
    34141 taatctcatt acgcaaacgg aaatcgccct cagccagtcg cgcagccaga aggagctgga
    34201 agatgtgctc tactctaatc tcgaagaact gacgcgaatg gcgaaaatgg tcagcgatat
    34261 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    34321 ggcggatgaa gtcggcaaag tgttcgattt tttcgaggcg ttagcagaag atcgcggcgt
    34381 ggagctgcgg tttgttggcg acgagtgtca ggttgcgggc gatccgctga tgctgcgtcg
    34441 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccggag agacaattgt
    34501 tgtgcgctgc cagacggtcg atcacctggt gcaagttacc gtcgaaaacc ccggtacgcc
    34561 cattgcgccc gaacacttac cgcgattgtt tgaccgtttc taccgcgttg atccttcccg
    34621 ccagcgaaaa ggcgaaggta gcggcattgg cctggcgata gtgaaatcga ttgttgtcgc
    34681 gcataaaggc acagttgcgg taacgtcaga tgctcggggg acaaggtttg ttatcatatt
    34741 acccgcttaa tccttcagca aacggcaact tttataacca gtgtaaaaat aacatgccgc
    34801 aatagtaata cctattaatg ataaaaagct gtcacaattc ataaaaaacc ttaatatacg
    34861 ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg tggactggct tcttgatgtt
    34921 tttgctacct ggctctacgg cttaaaagta atcgcgataa cgttagcggt cattatgttc
    34981 atcagcgggc tggacgattt ttttattgat gtcgtctact gggtacgccg cattaaacgc
    35041 aagttgagtg tttatcgccg ctacccgcga atgagttacc gcgaactgta taaaccagat
    35101 gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg
    35161 gccgagctgg cggcgaccac gctcgactac gaaaactatc atatctttgt tggcacctac
    35221 cccaacgacc ccgatactca gcgtgatgtt gacgaagtgt gcgctcgctt cccgaacgtg
    35281 cataaggtag tctgcgcgcg tcctggcccc accagcaaag ccgactgtct gaacaacgtg
    35341 ctggacgcca tcacccaatt tgaacgtagc gccaatttcg cttttgctgg ttttattctg
    35401 catgacgccg aagatgtgat ttcaccaatg gaattgcgtc tgttcaacta tctggtcgag
    35461 cgtaaagatc tgattcagat cccagtgtat ccgttcgaac gcgaatggac gcacttcacc
    35521 agcatgacct acattgatga gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa
    35581 gccctcgccg gacaggtgcc cagcgcagga gtcggcacct gtttcagccg ccgcgccgtg
    35641 accgcactgt tagctgacgg tgacggtatt gctttcgacg tgcagagtct gactgaagat
    35701 tacgacattg gcttccgcct gaaagagaaa ggtatgacgg aaatttttgt ccgttttccg
    35761 gtggtggacg aagccaaaga acgcgagcag cgtaaatttt tacagcatgc gcggacgtca
    35821 aacatgatct gtgtgcgcga atatttcccc gatacctttt cgactgcggt tcggcaaaaa
    35881 tctcgctgga tcatcggcat tgttttccag ggctttaaaa cccacaaatg gacctccagc
    35941 ctgacgctga actactttct ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc
    36001 ttcctcgcga tgctggtgat gatccagctt ttgctgttgc tggcgtatga aagtttgtgg
    36061 cccgatgcct ggcatttcct ttctattttc agcggcagcg catggttaat gactctgctg
    36121 tggctaaact ttggcttgat gattaaccgc atcgtgcagc gggtgatttt cgttactggc
    36181 tactacggcc tgacgcaggg gctgctatct gtcctgcgtc ttttctgggg caacctgatt
    36241 aacttcatgg ccaactggcg cgcgttaaaa caggtacttc aacacggcga tccacgtcgt
    36301 gtcgcgtggg ataaaacaac gcatgacttc cccagcgtga ctggcgatac ccgctcgttg
    36361 cgtccgttag gtcaaatcct gctggaaaat caggtcatca ctgaagaaca actcgataca
    36421 gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt caatgctgat gcaggggctg
    36481 attagcgccg agcaactggc acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc
    36541 atcgatgcct ggcagatccc ttcctcgctg attgccgaaa tgccggcctc cgtggcgttg
    36601 cattatgcgg tactgccgct gcgtctggac aatgatgagt taattgtcgg cagtgaagat
    36661 ggtattgacc cggtttcgct ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac
    36721 gtcattgttc tgcgaggaca aattgtcacc ggattacgcc actggtatgc acgccgacgc
    36781 ggtcacgatc cgcgggcaat gttgtacaat gcagttcagc atcagtggct cacggaacag
    36841 caggccggtg aaatctggcg gcaatatgtg ccgcatcagt tcctgttcgc agaaatactg
    36901 accacgctcg gtcatattaa tcgttcagca attaacgtgt tgttattgcg ccatgaacgc
    36961 agttctctgc cgctcggcaa gtttttggtc accgaaggcg ttattagcca ggaaacgttg
    37021 gatcgcgtcc tgacaattca acgcgaatta caagtttcga tgcaatcact attactcaaa
    37081 gcaggtttaa acactgaaca ggttgcacaa ctggagtccg aaaatgaagg agaataacct
    37141 taatcgcgtc atcggatggt ctggtttact gctgacatct ttattgagta ccagcgcact
    37201 cgcagacaat atcggcacca gcgcagaaga gctggggctg agcgattatc gccattttgt
    37261 tatttatccc cgtctcgata aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc
    37321 catccgcgaa tttgaatata tacaccagca ggtgccggat aatattccgc tgactttata
    37381 ccttgcggaa gcctatcgcc attttggtca tgatgaccgg gcgcggctgt tgcttgagga
    37441 tcaactgaaa cgtcaccctg gagatgaccg gcttgagcgc agtctggcgg ctattccagt
    37501 tgaagtgaaa agcgttacga ctgttgaaga actgcttgcc cagcaaaaag cgtgcgatgc
    37561 tgcgccgacc ctgcgttgtc gcagtgaagt cgggcagaat gccctgcggc tggcacaatt
    37621 acctgtcgcc agagcgcaac ttaacgatgc gacgtttgct gcatcgccgg aaggaaaaac
    37681 gttgcgaacc gatctgctgc aacgggcaat ctacctgaaa caatggtccc aggcagatac
    37741 gctatataat gaagcacgcc agcagaacac attaagcgcg gcagaacgcc gtcagtggtt
    37801 tgacgtgctt cttgccgcgc agctggatga tcggatcctg gcgctgcaat cacaggggat
    37861 cttcaccgat cctcagtcat atattactta tgcgaccgcg ctggcttatc gtggcgaaaa
    37921 agcacgcctc cagcattatc tcattgaaaa taagccgctg tttaccacgg acgcacaaga
    37981 gaaaagttgg ctctatctgt tatctaaata cagcgccaac cccgttcagg cgttggcgaa
    38041 ttatacggtg cagtttgccg ataaccgcca gtatgttgtt ggcgcgacgc taccagtgct
    38101 gttaaaagaa ggtcagtacg acgcagcgca aaaactgctc gccaccctcc ccgccaatga
    38161 aatgcttgag gagcgttatg ccgttagcgt agcaacccgt aacaaggctg aagctctgcg
    38221 tctggcacga ttgctgtatc agcaagaacc ggcaagtctt acccgtctgg atcaactgac
    38281 ctggcaactg atgcagaacg ggcagtcacg cgaagctgcc gatttattgc tgcaacgcta
    38341 tccttttcag ggcgatgcgc gtgtcagcca gacgttaatg gcgcgactgg cgtctctgct
    38401 ggaaagtcat ccttacctgg caacgccggc gaaggtggca attttatcga aacctttacc
    38461 gctggcggag caacgtcagt ggcaaagtca gttgccgggt attgctgata attgcccggc
    38521 aatagttcgc ttgctgggcg atatgtcgcc ttcttacgat gctgccgcct ggaaccgtct
    38581 ggcaaagtgt tatcgggaca cgctacccgg tgtggcgttg tatgcatggc ttcaggccga
    38641 acaacgacaa ccggacgcct ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga
    38701 ggactatgcc accgcactga cagcctggca gaaaatcagt cttcacgaca tgagcaatga
    38761 ggatctgctt gctgccgcca ataccgccca ggcggcagga aatggcgcgg ctcgcgatcg
    38821 ctggttacaa caggcagaac aacgtggact gggaaacaat gccctctact ggtggctgca
    38881 tgcgcaacgt tacattccag gtcagccgga actcgcactg aacgatctca cgcgctcaat
    38941 caatattgcg ccttctgcca acgcttacgt tgcgcgggca acaatttatc gccaacgtca
    39001 taatatcccg gcggcggtga gtgatttgcg cgccgcgctg gaactggaac cgaataatag
    39061 caacacccag gcagcgcttg gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg
    39121 ggaaatgctc gaacaggcgc ataaagggct accggacgat ccggcactta tccgacaact
    39181 ggcctacgtg aaccaacgtc tggatgacat gcctgcgacg cagcactacg cccgactggt
    39241 gattgatgac attgataatc aggcgctgat aaccccactg accccagagc aaaatcagca
    39301 acgcttcaat ttccgccgtc tgcatgagga ggtcggtcgc cgctggtcgt tcagtttcga
    39361 ttcttctatc ggcttgcgtt ccggcgcaat gagtaccgct aacaataatg tcggcggcgc
    39421 agcgccaggg aaaagctatc gtagctacgg gcaactggaa gccgagtacc gcctcggacg
    39481 caatatgctg ctggaaggcg acctgctctc agtttatagc cgcgtctttg ccgataccgg
    39541 tgaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc ggcaccggtc tgcgctggaa
    39601 gccactgcgc gatcagatct ttttcctcgc cgtcgaacag cagttgccgc tgaacggaca
    39661 aaatggcgca tccgatacca tgctgcgcgc cagcgcctca ttctttaatg gcggcaaata
    39721 cagcgacgaa tggcacccga acggttcagg ctggtttgcc caaaacctgt acctcgatgc
    39781 ggcgcaatat atccgccagg atattcaggc gtggacggca gattatcgcg tcagctggca
    39841 tcagaaggtg gcgaacgggc agactattga gccttatgct cacgttcagg acaacggcta
    39901 tcgtgataaa ggcactcagg gcgcgcagct tggcggtgtc ggggtccgct ggaatatctg
    39961 gaccggcgag acgcactacg acgcctggcc gcacaaagtc agtctcggcg tcgaatatca
    40021 acataccttt aaggcgatta atcaacgtaa cggagagcgc aacaacgcgt ttctcaccat
    40081 tggagtgcac tggtaaatgc gtaagttcat tttcgtattg ctgacactgc ttttggtcag
    40141 ccctttttcc tttgcgatga aaggtattat ctggcaacca caaaaccgtg atagtcaggt
    40201 taccgatacc cagtggcagg ggctgatgag tcagttacgt ttgcaaggct tcgatacact
    40261 ggttttgcaa tggacccgtt acggcgatgc attcacccag ccagaacagc gcgcgttatt
    40321 gtttaagcgt gctacagctg cgcaacaggc tggcctgaag ctgattgtcg ggctgaacgc
    40381 cgatccggaa ttttttatgc accagaaaca gtcgtccgca gcgctggaaa gctatcttaa
    40441 tcgcctgctg gctgccgatc tccagcaagc cagattatgg agcgccgtgc ctggcgtaac
    40501 gccggatggc tggtacatca gcgcggaaat tgacgacctg aactggcgca gcgaagccgc
    40561 ccgtcagcct ttgctaacat ggttaaacaa cgcgcagcgg ttgattagcg atgtttcagc
    40621 aaaaccggtt tatatcagta tttttttcgc cggaaacatg tcgccagatg gctatcgcca
    40681 actgctggaa caagttaaag caaccggtgt taatgtctgg gtacaggatg gcagcggcgt
    40741 ggataaactg accgctgaac agcgtgaacg ttatctacag gccagcgccg attgccaaag
    40801 ttccgcccca gcaagcggca ttgtttatga actgtttgtc gctggtaaag gtaaaacgtt
    40861 tacagcgaaa ccgaaaccgg atgcagagat cgcctcgctg ttagcgaaac gttcctcttg
    40921 cggtaaagac acgctctatt tctctctgcg ctatttgccc gtcgcgcaga gtatccttga
    40981 gtattaatga ttaaagggag caacttagct tgctcctttc cttttcactt tcctaatcaa
    41041 aatcctggca agatgaatta tttagcagaa tatctgtttt cattttattc gtctgacgtg
    41101 ttccaattat ttagcaaaac cgcgacaaca aagtgatggt gtaatttgta aaagggaaca
    41161 aataaatggc tcgccctgca ggattcgaac ctgtagccca cggcttagaa actccaggaa
    41221 cgacatttta agtcaacaaa ttaccgcgcc atctctgcgt tcacacgtcc cactacctca
    41281 aaatatgtaa aaccttgcaa gccattgcga ggccttatgt gtctcagttt tgttcctctt
    41341 ttttatacta aaaaacacag caattgagga taaacctcat gctattatcg ctaatatgca
    41401 tctaaaggca tggcacttga atagataaaa actccacaaa gaaaaaaata attagcccat
    41461 aaaaccacaa tgcaaaacac aaacagacaa ttaaactaaa aaact
//
LOCUS       DAPQZD010000021        40364 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPQZD010000021 DAPQZD010000000
VERSION     DAPQZD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.021"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            157..1374
                     /gene="alaA"
                     /locus_tag="RO454_001128"
     CDS             157..1374
                     /gene="alaA"
                     /locus_tag="RO454_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDY0932995.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1458..2057
                     /gene="yfbR"
                     /locus_tag="RO454_001129"
     CDS             1458..2057
                     /gene="yfbR"
                     /locus_tag="RO454_001129"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDY0932996.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2116..3948)
                     /locus_tag="RO454_001130"
     CDS             complement(2116..3948)
                     /locus_tag="RO454_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDY0932997.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4035..4685)
                     /gene="hxpA"
                     /locus_tag="RO454_001131"
     CDS             complement(4035..4685)
                     /gene="hxpA"
                     /locus_tag="RO454_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDY0932998.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4696..5190)
                     /locus_tag="RO454_001132"
     CDS             complement(4696..5190)
                     /locus_tag="RO454_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDY0932999.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5273..5728)
                     /locus_tag="RO454_001133"
     CDS             complement(5273..5728)
                     /locus_tag="RO454_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDY0933000.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6066..7268
                     /gene="ackA"
                     /locus_tag="RO454_001134"
     CDS             6066..7268
                     /gene="ackA"
                     /locus_tag="RO454_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDY0933001.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7343..9487
                     /gene="pta"
                     /locus_tag="RO454_001135"
     CDS             7343..9487
                     /gene="pta"
                     /locus_tag="RO454_001135"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDY0933002.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9677..11197
                     /gene="yfcC"
                     /locus_tag="RO454_001136"
     CDS             9677..11197
                     /gene="yfcC"
                     /locus_tag="RO454_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDY0933003.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11230..11772)
                     /gene="yfcD"
                     /locus_tag="RO454_001137"
     CDS             complement(11230..11772)
                     /gene="yfcD"
                     /locus_tag="RO454_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDY0933004.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11830..12381)
                     /gene="yfcE"
                     /locus_tag="RO454_001138"
     CDS             complement(11830..12381)
                     /gene="yfcE"
                     /locus_tag="RO454_001138"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDY0933005.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12437..13081)
                     /gene="yfcF"
                     /locus_tag="RO454_001139"
     CDS             complement(12437..13081)
                     /gene="yfcF"
                     /locus_tag="RO454_001139"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDY0933006.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13217..13864
                     /gene="yfcG"
                     /locus_tag="RO454_001140"
     CDS             13217..13864
                     /gene="yfcG"
                     /locus_tag="RO454_001140"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDY0933007.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13921..14283
                     /gene="folX"
                     /locus_tag="RO454_001141"
     CDS             13921..14283
                     /gene="folX"
                     /locus_tag="RO454_001141"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDY0933008.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            14304..15197
                     /locus_tag="RO454_001142"
     CDS             14304..15197
                     /locus_tag="RO454_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDY0933009.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15356..16129)
                     /gene="hisP"
                     /locus_tag="RO454_001143"
     CDS             complement(15356..16129)
                     /gene="hisP"
                     /locus_tag="RO454_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDY0933010.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16137..16853)
                     /locus_tag="RO454_001144"
     CDS             complement(16137..16853)
                     /locus_tag="RO454_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0933011.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(16850..17536)
                     /gene="hisQ"
                     /locus_tag="RO454_001145"
     CDS             complement(16850..17536)
                     /gene="hisQ"
                     /locus_tag="RO454_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDY0933012.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17626..18408)
                     /gene="hisJ"
                     /locus_tag="RO454_001146"
     CDS             complement(17626..18408)
                     /gene="hisJ"
                     /locus_tag="RO454_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDY0933013.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18629..19411)
                     /gene="argT"
                     /locus_tag="RO454_001147"
     CDS             complement(18629..19411)
                     /gene="argT"
                     /locus_tag="RO454_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDY0933014.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19677..20246)
                     /gene="ubiX"
                     /locus_tag="RO454_001148"
     CDS             complement(19677..20246)
                     /gene="ubiX"
                     /locus_tag="RO454_001148"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDY0933015.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(20341..21858)
                     /gene="purF"
                     /locus_tag="RO454_001149"
     CDS             complement(20341..21858)
                     /gene="purF"
                     /locus_tag="RO454_001149"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDY0933016.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(21895..22383)
                     /gene="cvpA"
                     /locus_tag="RO454_001150"
     CDS             complement(21895..22383)
                     /gene="cvpA"
                     /locus_tag="RO454_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDY0933017.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(22824..23486)
                     /gene="dedD"
                     /locus_tag="RO454_001151"
     CDS             complement(22824..23486)
                     /gene="dedD"
                     /locus_tag="RO454_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDY0933018.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(23476..24744)
                     /gene="folC"
                     /locus_tag="RO454_001152"
     CDS             complement(23476..24744)
                     /gene="folC"
                     /locus_tag="RO454_001152"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDY0933019.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(24814..25728)
                     /gene="accD"
                     /locus_tag="RO454_001153"
     CDS             complement(24814..25728)
                     /gene="accD"
                     /locus_tag="RO454_001153"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDY0933020.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(25884..26543)
                     /locus_tag="RO454_001154"
     CDS             complement(25884..26543)
                     /locus_tag="RO454_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0933021.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(26626..27438)
                     /gene="truA"
                     /locus_tag="RO454_001155"
     CDS             complement(26626..27438)
                     /gene="truA"
                     /locus_tag="RO454_001155"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDY0933022.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(27438..28451)
                     /locus_tag="RO454_001156"
     CDS             complement(27438..28451)
                     /locus_tag="RO454_001156"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDY0933023.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(28517..29653)
                     /gene="pdxB"
                     /locus_tag="RO454_001157"
     CDS             complement(28517..29653)
                     /gene="pdxB"
                     /locus_tag="RO454_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDY0933024.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            29752..30747
                     /gene="flk"
                     /locus_tag="RO454_001158"
     CDS             29752..30747
                     /gene="flk"
                     /locus_tag="RO454_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDY0933025.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(30744..31922)
                     /locus_tag="RO454_001159"
     CDS             complement(30744..31922)
                     /locus_tag="RO454_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDY0933026.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32187..33407)
                     /gene="fabB"
                     /locus_tag="RO454_001160"
     CDS             complement(32187..33407)
                     /gene="fabB"
                     /locus_tag="RO454_001160"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDY0933027.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            33566..35572
                     /gene="mnmC"
                     /locus_tag="RO454_001161"
     CDS             33566..35572
                     /gene="mnmC"
                     /locus_tag="RO454_001161"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDY0933028.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(35693..35971)
                     /locus_tag="RO454_001162"
     CDS             complement(35693..35971)
                     /locus_tag="RO454_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDY0933029.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(36005..36553)
                     /gene="epmC"
                     /locus_tag="RO454_001163"
     CDS             complement(36005..36553)
                     /gene="epmC"
                     /locus_tag="RO454_001163"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDY0933030.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(36553..37362)
                     /locus_tag="RO454_001164"
     CDS             complement(36553..37362)
                     /locus_tag="RO454_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDY0933031.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(37362..38186)
                     /gene="mepA"
                     /locus_tag="RO454_001165"
     CDS             complement(37362..38186)
                     /gene="mepA"
                     /locus_tag="RO454_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDY0933032.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38190..39275)
                     /gene="aroC"
                     /locus_tag="RO454_001166"
     CDS             complement(38190..39275)
                     /gene="aroC"
                     /locus_tag="RO454_001166"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDY0933033.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(39310..40242)
                     /gene="prmB"
                     /locus_tag="RO454_001167"
     CDS             complement(39310..40242)
                     /gene="prmB"
                     /locus_tag="RO454_001167"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDY0933034.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
BASE COUNT         9893 a        11015 c        10127 g         9329 t
ORIGIN      
        1 tttgccatta ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt
       61 ctgcagacct tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc
      121 agggagtggc gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc
      181 aaattagaga atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg
      241 gaagaagaag gtaacaaggt tctgaaactg aacatcggca accctgcccc gttcggtttt
      301 gacgcgccag atgaaattct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat
      361 tgcgattcca aaggtcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt
      421 ggcatgcgtg atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc
      481 gttcaggcaa tgcaagcatt gctgaacagc ggggacgaaa tgttggttcc tgcacctgat
      541 tacccactat ggaccgcggc ggtttcactc tccagcggta aagcggtgca ttatctttgc
      601 gatgaatcct ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt
      661 acgcgtggga tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag
      721 cttttaatgg agattgtgga gattgcacgt cagcataatc tcattatctt tgccgatgaa
      781 atttatgaca aaattctcta cgacgacgct gagcatcact ccattgcgcc gcttgcacct
      841 gacctgctga ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt
      901 caggggtgga tggtgttgaa cgggccgaaa aaacacgcta aaggttacat cgaaggtctg
      961 gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc
     1021 gcactgggtg gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag
     1081 cagcgtaacc gcgcgtggga attgatcaac gatattccgg gcgtttcctg cgtgaaacct
     1141 cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat
     1201 cagaaaatgg tgctggattt cctgttgcag gaaaaagtcc tgttggtgca agggacggca
     1261 ttcaactggc cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc
     1321 gagctgtctt tgagtaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa
     1381 tttcactgcc ggagattgca tccggcagtg ttatcccgcc acaatgacct gatgatgtca
     1441 tcatacgtaa ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac
     1501 tcattaaccg ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt
     1561 tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa aaatcgtaaa tttggcggta
     1621 atgtcaacgc cgaacgtatc gctttactgg cgatgtacca cgatgcttca gaagtgctca
     1681 ccggagatct ccctactccg gtgaaatact tcaattcgca aatcgctcag gaatacaagg
     1741 ctattgaaaa aatcgctcag caaaaactgg tcgatatggt tccggaagag ctacaggata
     1801 tctttgcgcc gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac
     1861 aggcagatgc actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg
     1921 aattcttgct ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga
     1981 tggactactt catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc
     2041 aggattcacc gctgtaagca gctggagtct gcgtcgcatc aggcaataag cgccggatgc
     2101 gacatcaggc tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat
     2161 cacgataatg gtgaacggta cccccaactt aacaaagtca ctgaagctgt aattccccgg
     2221 acctaaaacc agtgtgttaa caggtgaaga tatcggtgtc ataaaggcgg cggatgctgc
     2281 catcgccacg accatcgcaa atggataagg cgacaccccc atcgttttgg cagcagccag
     2341 cgcaatcgga gccatcaaca ccgccgtcgc agtattagaa ataaatagcc caataaccgc
     2401 cgacaagaca aacaaacaac ccagcatcat atgtggccca taaccgccgc caatgtccat
     2461 cagccctttc accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat
     2521 catcccaacg atcaaaataa tgctcggcca gtgaatggat ttataggcac tttcggcatc
     2581 gatacagcgg aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat
     2641 ttcatcggtc agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg
     2701 gctgtgcgcg ggcgatgctt cactcacctc ttccggtaag ttcagcgcca cgaagtcgcg
     2761 gccctgtttg gccagcatac cgatcagttt ccagttacca acaaccagaa tgatatcgcc
     2821 cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc acaccattgc gctttagccc
     2881 caccacattc aatccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaataag
     2941 ttctgactca ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa
     3001 atactcgccg cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc
     3061 gacatcagcc gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc
     3121 attaacgttc acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc
     3181 accataacgt tcacgtaatt tgagatcgtc cagtcgttga ccaatcattg gcgatccggg
     3241 gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa
     3301 ggttcggcgc gtccagcctt cgcgttgtgg ggtctgggta tcccctttca gcatgaaacg
     3361 catcactaac atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct
     3421 aaagaaactg aagccgtgat agccttcacg cagtaattca ctgttgacta ccaggttcgg
     3481 cggcgtcgcc accagcgtca tcatgccgct tatcagcccg gcaaaactta acggcatcat
     3541 cagacgcgac ggcgacgttt gcatacgcat ggcaacgctt aacaccacgg ggataaagat
     3601 agcgacaacg ccggttgaac tcataaacgc gccaagtccc gcgacggtca gcatcaacaa
     3661 aaccaacatt ttgatttcac tattgcccgc cactttgacc agccatgtcc ccattacagt
     3721 ggcaacaccg gtacggacca aaccatcgcc aataatgaac aaggcggcaa tcaggacaac
     3781 gttaggatcg gaaaagccgg aaaatacttc ggggaccgtc agcgttccgc ttaatgcaaa
     3841 cgcgacaata acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac
     3901 gatggcaacg gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg
     3961 ttaattgagg gggataactt gattctgcca taaaaaagcc ccgacgagac ggggctaaat
     4021 catgatcagg tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt
     4081 tgctccagac tgtggaggac taaatcgact tcattcaggc gcggggtatc tgccggggca
     4141 ttaaccgcaa taacatggca acccgccgcc agacctgaaa gcacgccagc gggagcatct
     4201 tccaccacaa cacactcctg cggcgcaagc cccagcagct gcgcgcctaa cagatacgca
     4261 tcaggttctg gttttccgcg cttcacccgc tcagcggtta caaacacctc tggcgcggga
     4321 agccccgcta ttttatgacg cgctcgcgct accggcatgg agccagaagt cacaatagcc
     4381 cacggaatac ctgctttatt caaatgattg agtaaggcga ttgcccccgg aagcgcggta
     4441 ataccttcgg tttccgtggc ctcgatgtgt tccagacgcg taaactcggc ggcaatgtct
     4501 gcctcggatt tgcccgccat aaaatggcgc agagaggtga tcgcctgttt accgtgaatg
     4561 aaagccagca cctcttccgg cgataaccca tgacgtctgg cccagttgct ccacgcccgt
     4621 tctaccgcag gcagggaatc caccagcgtt ccatcaagat caaacagaaa acctttgcac
     4681 cgcacgcggg cctcctcagg cgttaataat ttgattaatt tcgttggcgc tcaaatggta
     4741 ctgacgcggg caggcatgcc acacattaag catgcgctga tatttttccc acattggcgt
     4801 ctgggcgtta aaaccgtgag ttccagcgtc aaaatgggta tagcgccctt ccacattaac
     4861 cataaagcgg acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc cgaggaaggt
     4921 gacacgacgt tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa catgcaacgc
     4981 atgatacatc tccataatgt cgatgatagt gcggcaggtt tcttctttca gctcgccaaa
     5041 ctcgcgatcc agttcacgca tctgtaatcc ataaccacgc tcaatgattg tttgcaggcg
     5101 acggtaacgt tcagcatttg ccggatcgag catagtcatc atcttgtact ggttagacaa
     5161 aataagacgt tgcgcgttgg tcatttccat tattgactcc tgtatcactc tactacggtg
     5221 aaaaaaaaga aggctgagta tgccttcttt tatatgcgta atcaggggtc aattacaaat
     5281 catcaaggaa agttttatcc agttgtttga aggcacgctt aagcgtgtca gctaatgcct
     5341 ggtaatcagg cttgccttca acgggtgcca acacctgtcc agactcctgc aatttaccgc
     5401 gaacttcata aaaccagttg aggattgcag gaggtaatgg cgttacagaa cgcttgccca
     5461 gccaccacaa tccctgcatg ggtaaactta aggcgaacag cgcagtggca actgccggcc
     5521 caagctgacc gcccagggca atctgccagc agagagtaaa tacggcgatc ggcggcataa
     5581 aacggatcgc ataacgcgtc atcttgataa cgcgattttc gacaaagact ggggcaaggc
     5641 gtttttccag cggccacgtc tttgagtaat gctgtccccg gcgaaacaag ctaaaaaaat
     5701 taacagaacg attatccggc gttgacatgc ttcacctcaa cttcacatat aaagattcaa
     5761 aaatttgtgc aaattcacaa ctcagcggga caacgttcaa aacattttgt cttccatacc
     5821 cactatcagg tatcctttag cagcctgaag gcctaagtag tacatattca ttgagtcgtc
     5881 aaattcatat acattatgcc attggctgaa aattacgcaa aatggcatag actcaagata
     5941 tttcttccat catgcaaaaa aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc
     6001 atcatgcgct acgctctatg gctccctgac gtttttttag ccacgtatca attataggta
     6061 cttccatgtc gagtaagtta gtactggttc tgaactgcgg tagttcttca ctgaaatttg
     6121 ccatcatcga tgcagtaaat ggtgaagagt acctttctgg tttagccgaa tgtttccacc
     6181 tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa acaggaagcg gctttaggtg
     6241 caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa tactattctg gcacaaaaac
     6301 cagaactgtc tgcgcagctg actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt
     6361 ataccagctc cgtagtgatt gatgagtctg ttattcaggg tatcaaagat gcagcttctt
     6421 ttgcaccgct gcacaacccg gctcacctga tcggtatcga agaagctctg aaatctttcc
     6481 cacagctgaa agacaaaaac gttgctgtat ttgacaccgc attccaccag actatgccgg
     6541 aagagtctta tctctacgcc ctgccgtaca acctgtacaa agagcacggc atccgtcgtt
     6601 acggcgcgca cggcaccagc cacttctatg taacccagga agcggcaaaa atgctgaaca
     6661 aaccggtaga agaactgaac atcatcacct gccacctggg caacggtggt tccgtttctg
     6721 ctatccgcaa cggtaaatgc gttgacacct ctatgggcct gaccccgctg gaaggtctgg
     6781 tcatgggtac ccgttctggt gatatcgatc cggcgatcat cttccacctg cacgacaccc
     6841 tgggcatgag cgttgacgca atcaacaaac tgctgaccaa agagtctggc ctgctgggtc
     6901 tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg
     6961 cgaagcgcgc aatggacgtt tactgccacc gcctggccaa atacatcggt gcctacactg
     7021 cgctgatgga tggtcgtctg gacgctgttg tattcaccgg tggtatcggt gaaaatgccg
     7081 cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg
     7141 aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc
     7201 ctgcggtggt tatcccaacc aacgaagaac tggttatcgc gcaagacgcg agccgcctga
     7261 ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg
     7321 cggtaacgaa agaggataaa ccgtgtcccg tattattatg ctgatcccta ccggaaccag
     7381 cgtcggtctg accagcgtca gccttggcgt gatccgtgca atggaacgca aaggcgttcg
     7441 tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac
     7501 tacgactatc gtgcgtgcga actcttccac cacgacggcc gctgaaccgc tgaaaatgag
     7561 ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag agatcatcgc
     7621 gaactaccac gctaacacca aagacgctga agtcgttctg gtggaaggtc tggtcccgac
     7681 acgtaagcac cagtttgccc agtctctgaa ctacgaaatc gccaaaacgc tgaacgcaga
     7741 aatcgtcttc gttatgtctc agggcactga tactccggaa cagttgaaag agcgtatcga
     7801 actgactcgc aacagcttcg gcggtgcaaa aaacaccaat attaccggcg ttatcgttaa
     7861 caaactgaac gctccggttg atgagcaggg tcgtacccgt ccggatctgt ccgagatttt
     7921 tgacgactcc accaaagcaa aagtgaacaa cgttgatccg gcgaagctgc aagaatccag
     7981 cccgctgccg gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat
     8041 cgatatggct cgccacctga atgcgaccat catcaacgaa ggcgacatca atactcgccg
     8101 cgttaaatcc gtcactttct gcgcacgcag cattccgcac atgctggagc acttccgtgc
     8161 cggttctctg ctggtgactt ccgcagaccg ccctgacgtg ctggttgccg cttgcctggc
     8221 tgccatgaac ggcgtagaaa tcggtgccct gctgctgact ggcggctacg aaatggacgc
     8281 gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa
     8341 caccaacacc tggcagactt ctctgagcct gcagagcttc aacctggaag ttccggttga
     8401 cgatcacgag cgtatcgaga aagttcagga atacgttgct aactacatca acgctgactg
     8461 gatcgattct ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc cagcgttccg
     8521 ttatcagctg actgaacttg cgcgcaaagc gggcaaacgt atcgttctgc cggaaggtga
     8581 cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt
     8641 actgctgggt aatccggcag agatcaaccg tgttgcagcc tctcagggtg tagaactggg
     8701 tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa aactatgttg gtcgtctggt
     8761 cgaactgcgt aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa
     8821 cgtggttctc ggtacgctga tgctggaaca agatgaagtt gatggtctgg tttccggtgc
     8881 tgttcacacc accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg
     8941 cagctccctg gtatcttccg tgttcttcat gctgttgccg gaacaggttt acgtttacgg
     9001 tgactgtgcg atcaacccgg atccgaccgc agaacagctg gcagaaatcg cgattcagtc
     9061 cgctgattcc gctgcggcct tcggtatcga accgcgcgtt gctatgctct cctactccac
     9121 cggtacttct ggtgctggta gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca
     9181 ggaaaaacgt cctgatctga tgatcgacgg tccgctgcag tacgacgctg cggtaatggc
     9241 tgacgttgcg aaatccaaag caccgaactc tccggttgca ggtcgcgcta ccgtgttcat
     9301 cttcccggat ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt ctgctgacct
     9361 gatctctatc ggaccgatgc tgcagggcat gcgcaagccg gttaacgacc tgtcccgtgg
     9421 cgcactggtt gatgatatcg tctacaccat cgcgctgact gcaattcagt ctgcacagca
     9481 gcagtaatct cgtcatcatc cgcagctttg cgctgcggat atctgaaccg gaaatagtca
     9541 ctatttccgg ttttttattc tcttaatttg cattaatcct ttctgattat cttgcttaac
     9601 tgcgctgcat caatgaattg cgccatctca ctttgcatac ttaccacttt gttttgtgca
     9661 agggaatatt tgcgctatgt ccgcaatcac tgaatctaaa ccaacaagaa gatgggcaat
     9721 gcccgatacg ttggtgatta tcttttttgt tgccatttta accagccttg ccacctgggt
     9781 agttccggtg ggcatgtttg acagtcagga agtgcagtat caggttgatg gtcaaacaaa
     9841 aacacgcaaa gtcgtagatc cacactcatt tcgcattctg actaacgaag caggcgaacc
     9901 tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa cgcccgggtc tgatgaactt
     9961 cccgtttgaa gggttaacct caggatcgaa atacgggaca gccgttggca tcatcatgtt
    10021 tatgctggtg attggcggcg cgtttggcat tgtgatgcgt acaggaacca ttgataacgg
    10081 tatcctggcg cttattcgcc atacccgcgg aaatgaaatt ctctttattc ctgcgctgtt
    10141 tattctgttt tcacttggcg gcgcaatatt tggtatggga gaagaggccg tcgcctttgc
    10201 cattatcatc gcaccgctaa tggtccggct gggctatgac agtattacca ccgtccttgt
    10261 gacctatatt gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt
    10321 tgttgctcag ggtattgccg gcgttccggt gctttctggc tccgggttgc gcatcgtggt
    10381 gtgggttatc gccactctga ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa
    10441 aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc
    10501 tgatgttgaa caacgtccgt ttacctttgg tgactggctg gtattgattg tcctgaccgc
    10561 cgtaatggtc tgggttattt ggggcgtgat cgttaatgcc tggtttattc ctgaaattgc
    10621 cagccagttc ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt tccgccttaa
    10681 cggcatgacg gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc
    10741 ccctgccctg ctggtgggtt tcgccaaagg gattttgctg ctggtcggta atggtgaagc
    10801 gggtgatgcc agcgtgttaa ataccatcct caacagcatt gccaatgcca ttagcggtct
    10861 ggataacgca gtcgcggcct ggtttatgtt gctcttccag gcggtattta atttcttcgt
    10921 gacgtccggt tctggtcagg cggcgttaac catgccgtta ctggcaccgc ttggcgatct
    10981 ggtcggtgtt aaccgtcagg ttaccgtgct ggcgttccag tttggtgatg gcttcagcca
    11041 catcatttac ccaacctcag cttcgttaat ggcgacgctc ggtgtttgcc gggtggactt
    11101 ccgtaactgg ctgaaggtgg gtgcgaccct gcttggactg ctgtttatta tgtccagcgt
    11161 cgtggtgatc ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc cgcgattgcg
    11221 gctctttttt cattctgccg tttcagtctc gacggcttca tttttggcat tgcgcttcat
    11281 ccacaacgcc agcgctttca gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc
    11341 ttccggcgtc agccagcaaa cttcactgac ttcatcttcc tgtagagcga agggaccgtg
    11401 agagacgcag ctgaacaacg cgccccagac acggcaattt ttatcttcga aatagaactg
    11461 cccgtgctcg gcaaagggga caccggcaat gcccaactct tcttccgctt cgcgacgcgc
    11521 ggattccagc agctgctcat cggcctggac tacaccgcct gcggtcgcat ctaacatgcc
    11581 gggtaaaaag tcttttgtct cggtacgacg ctggaccaga attttgccca tgccatcatg
    11641 cacgacgata taagttgcac gatgacgcag acactgtgcc cgcatttgtt cccggctggc
    11701 ttgtgcaatg acttcgttct cttcattgac aatatccacc cattcagtac ttgccaaacg
    11761 acgctgttcc attatcagga aaccttcttt ttctggcgct cttacggcgc attttgagtt
    11821 gtgggtaaat tacggattaa tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat
    11881 tacactaagt acgtcattat ccagcatgcc ataactcgcc ggattaccgc ctttcggaat
    11941 actcaccgag ccggggttga agtggaaaat ctccccccgc tgttctgcca ctggcagatg
    12001 ggtatgaccg tacaccagca catcgttctg gtttaaagca ggtagatttt ccgggccaaa
    12061 aagatgacca tgcgtcaaaa acagacgttg tttttccagt aatacctgtt gccacggcgc
    12121 ggttatcggg aaatgcagca gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc
    12181 gataacctta tgtgccactt cattaagccg ttcagcgact ttggctggcg cgtaaccctc
    12241 cggtaaagca ttacgcgggc catgattcaa cacgtcgcca aggatcacca gccactgggc
    12301 accgctttgg gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc
    12361 cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg
    12421 gattctgtcg ttgctatcag ccagattgct tcgccgaaag tgcaataaaa cgctggacag
    12481 acgctcgctg ccactggaat gtcgcataat ccaccaggcg ttccggcacc tcatcgccat
    12541 gtagtaccag gcggttaatc attagcgcca gatcagtatc agcaatgcac cattcaccaa
    12601 ataaattcgg ctgacccagt gctaacaaat gctctgccat cgcgaacagt ttctctgcac
    12661 tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg
    12721 ggcgttcttc gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc tgtcgtgcac
    12781 gcgcacgatt ttctaaatca agcggataaa tacgttccca ggtcggtggc gcaaatcgat
    12841 cttccagata ctccgcaatg gcagaagatt cactcaactc aaaatcatcg atttgtaata
    12901 acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg ctgcaaatgt tcaccgctgt
    12961 cgaggtcgat ggtcttgata tgaaacgaca ggcctttctc ctgcaacgcc acccaggcgg
    13021 ataacacata aggggagaaa aaatgggcat ctgaccaaag cgtaatagcg ggtttactca
    13081 ttatgtcctc ggcaataaag ggggcttgct atcaaactat agagcctttt tgtcactgtc
    13141 acgtgacgaa tactcacgca agtcgccgcg ctttctatac ttgtggtgtt cagatgatta
    13201 gacaaccgga gctgcaatga tcgatctcta tttcgccccg acacccaatg gtcacaaaat
    13261 tacgctgttt ctcgaagaag cagggctgga ttatcgcttg ataaaggtag acctggggaa
    13321 aggaggtcag tttcgcccgg aatttttgcg catttcgcct aacaacaaaa ttccggcaat
    13381 tgttgatcat tctccagccg atggcggcga accgctaagc ctctttgagt ctggtgccat
    13441 tttgttgtat ctggctgaga aaacgggact ctttttgaat catgaaacgc gtgaacgcgc
    13501 cgtcacatta cagtggttat tctggcaggt aggcggactg gggccgatgc ttgggcaaaa
    13561 tcatcatttt aatcacgcag ccccccaaac cattccttac gctattgaac gttatcaggt
    13621 tgaaactcag cgtctgtacc atgtactgaa caagcggctg gaaaattcgc cctggctggg
    13681 aggcgagaac tacagcattg cggatattgc ctgctggccg tgggttaatg cctggactcg
    13741 ccagcgaatt gacctcgcaa tgtatccggc agtcaagaac tggcatgagc ggatccgttc
    13801 gcgccctgcc accgggcagg cactgctaaa agcacaacac ggtgatgagc gttcggatag
    13861 ttaacagaaa caggttctcg tgtattattt cattctaagt aaaacaacgg agaacctgca
    13921 atggcacaac ctgccgctat tattcgtata aagaaccttc gtttacgtac gtttatcgga
    13981 attaaggaag aagaaattaa caaccgtcag gatattgtta tcaatgtgac gatccactac
    14041 cccgccgata aagcgcgcac tagcgaagat atcaacgatg cgctgaatta tcgcaccgta
    14101 acgaaaaaca ttattcagca tgtagagaat aaccgtttct ctttgctgga aaaattaact
    14161 caggatgtgc tcgatatcgc acgcgaacat cactgggtga cgtatgctga agtggagatc
    14221 gataaactgc acgcgctgcg ctacaccgat tcggtatcca tgaccttaag ctggcagcgt
    14281 taaccaccat atcgggaggc tgcatgaaca tagtgatcac cggagggacg ggattaattg
    14341 gtcgccattt gattccacgt ttgctggacc tgggccatca aatcacggta gtgacgcgta
    14401 acccgcagaa agccagttcc gttctcggcc cccgagtgac actatggcaa gggcttgccg
    14461 aacaaagcaa cctcaacggc gttgatgcgg taatcaacct ggccggagaa ccgattgctg
    14521 ataaacgctg gactcacgag cgaaaagagc gtctctgcca aagccgctgg aataccacgc
    14581 aaaaactggt cgatttgatt aatgccagcg acacgccacc gtcggtactc atttccggct
    14641 cggcaacggg ctattatggc gacttaggtg aagtggtggt taccgaagag gaaccgccgc
    14701 ataacgaatt tacccataaa ctctgcgccc gctgggaaga gattgcctgt cgggcgcaaa
    14761 gtgacaaaac gcgagtgtgc ctgctgcgta ccggcgtagt gctggcacca gacggtggta
    14821 tcctcggtaa aatgctgccg ccatttcgtt ttggcctggg cgggccgatt ggctccggtc
    14881 ggcagtatct ggcctggatt catattgatg atatggtcaa cggcattctc tggctgctgg
    14941 ataacgagct gcgcgggcca tttaatatgg tttcgcccta cccggtacgc aatgaacaat
    15001 ttgcccatgc gctcggtcat gcgctgcatc gcccgaccat tttgcgcgtc cccgcgaccg
    15061 ccattcgact gttaatgggc gaatcttcag tactggtatt aggtggacaa cgcgcgctgc
    15121 caaaaaagct ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa gaggcgctgg
    15181 cggatgtcgt tcgctgatgt ggtttactgc aaacatccgc cagcggaaac gctgatgaat
    15241 aaatatagat aattcagaac tatttttagc ggactgcaac ttttacattt cagcggaagc
    15301 ggactcgcaa attccccatg aaatactccg gcagcgttaa ccaccggaga ggaaattatt
    15361 tcagcgaacc tttaaggaac tgttgcagac gagggctttg cgggttaccg aataactgct
    15421 ccggcgcccc ttcttcttct attttcccct gatggaggaa aatgacgtga gtagaaacat
    15481 gacgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct tctgccagtt
    15541 gctgcataat acgcaacact tcgcctacca gttcaggatc gagcgctgaa gtaggttcat
    15601 caaacagtaa aacttccggt tccatcgcta gcgcccgcgc gatagaaaca cgctgttgct
    15661 gaccaccgga caggtgcacc gggtatttcc cctgcgcacg ttcgtctatc ccgacttttg
    15721 ccagatactt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg
    15781 gcgcttccat gacgttttcc agcaccgtca tatggctcca gagattgaag tgctgaaata
    15841 ccatcgtcag gcgtgtgcgc aataagcgca gctgattttt atcggcgact ttgagttgac
    15901 catctttatc gcgcaccaga ttgatcgtct ggccattgac cacgatcgat ccatcgctcg
    15961 gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga
    16021 tgatgcttat gacatctccg gcattcgctt gcaatgagac ccctttcagc acttcgtgtt
    16081 cgccgtagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag
    16141 tgcgttgaag aaggtttcac atgctgcaac cagcgttttt cagctctacg aaagaggctg
    16201 atcagaacat aagagatgat taaatagagc accgcggcaa tgccgaaagc ggtaaaaggt
    16261 tgatatgtgg cggcgttaat atcgcgggca attttcagca gatccggcac cgtggcagta
    16321 aatgccagcg cagtagagtg cagcatcagg atcacttcgt tgctgtatgc cggtaacgca
    16381 atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga gaagccatag
    16441 gctctggcgg cttcaatttc cccatgcggc actgaacgga ttgccccggc aaaaatctcg
    16501 gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag gccactgcga
    16561 aaaaaggcgt taaggaactc ggttccctta acaatctcca gcgtgtacat gccggaatag
    16621 aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt
    16681 ggaaactgga tgtatttatt acttgagacg cgaccaatcg ccagaaacag cgccagaact
    16741 ccgcctatca ctaccgacaa aatcagcagc cacagggtga tcgccacgcc agtaaagcga
    16801 taaccgtcag tccacagcag tggtttccag tattcatgta agatttcgat cacaggtcag
    16861 ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca ttggaaacgg
    16921 tggtgaaaac aagataaatc acgccgcaga cgatagcgaa atagaacggt tcccaggtac
    16981 ttttgcctgc cagctgcgtg gctttgacca catcttccag gccgagtaac gaaaccagtg
    17041 cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca
    17101 tcgccggaaa catgatccgc cgaaacactt gcccacgagt aaaaccgaac gccgtcgccg
    17161 cctctatatg tcctttcggc actgccataa acgcgccacg aaacgtttcg gtaaaataag
    17221 caccgtagat aaaaccgaga gtgataatac ctgcgaccat cggatcaata tcaatctgcc
    17281 cgacgcccat cgcctccgtc accgtgttta gcgcaatctg caaaccgtag aaaatcagca
    17341 gcatcaatac taaatccggc acgcctcgaa tcagcgtggt gtacccttcg aaaataaggc
    17401 ccgaaagccg attttgtgag agcttaccgc cagcgccaat taaaccgatg attacagcga
    17461 gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacttg
    17521 aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc
    17581 cctccggtta cccgaaggga gcgggctttc tttcaccgat ggcgattaac caccataaac
    17641 atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc
    17701 aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt
    17761 ccctacgcca aacagttttt catctttaac agacgggcca ccgaatttgt aatctttacc
    17821 gacgggttgt ttgaggaaac cttcgctggc tgcgacctca tcctggaacg cggcatcaat
    17881 acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgataagaaa cgatttcaat
    17941 gccttttggt gcccagtgtt cgttaccgaa cgtctcctgg gtggtgccct gcaatacgcc
    18001 tacccgtttg cctttcagcg attcgactgt cggctgaatg tcggaatttt tcgccaccac
    18061 cagacgagaa tcggctgcgt acagtttgtc ggtgaaggct atttcttgct gacgtttttc
    18121 cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa
    18181 cgcatccagt gggttttcga caaacgtaca ttgcgtattg atgcgtttgc acaactcctt
    18241 ggccagatcg atatcgaagc caaccagttc accttgtgaa ttttttgatt caaatggcgc
    18301 ataggtcgga tcggtaccga tgcggatgtt ttgcggaatc gcagcaaatg ctgcagttgc
    18361 gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac
    18421 tgacagtctt tttatcggga atatttacag gtttgttaca cttatcgtgc cataaaattg
    18481 accatcaagg caaaatatcc cgccccgcta gacgtattat ttttcattaa aaatgcttaa
    18541 gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt
    18601 ccatcaggta cagcttccct gcgacgtatc agtcaccgta gacattaaag tcaaaatact
    18661 ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc ttattaaacg
    18721 cagccgtcag ttccgcatca tctttacgta gccctacacc ggtgccgtca ccgaagtatt
    18781 ttttgtcttt cactgatggg cctgcaaagg cgaaatcttt accggcgggt tgcttaagga
    18841 aaccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct gcagccagat
    18901 cggaatagac taaatcctgg ttggcatagg ccaccacatc cacgcctttg ctacgccagg
    18961 tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt ttacctttca
    19021 gtgaatccag cgttggctga atcggtgaac ctttggcggc aatcaaacga gaatctgcgg
    19081 cgtacagctt gtcggagaaa gcaatctcct gctgacgttt atcggtaatg gagagcgacg
    19141 aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca aagtcgctgg
    19201 caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa
    19261 agccaacaaa gtcacctttg gcatctttcg atgagaacgg tgcgtaggtg gtatcggttc
    19321 cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag agaccgacta
    19381 acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt
    19441 tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta tgccatcttg
    19501 acgacagcag taacattcaa cgttaaatat gttaataaga agttgcatta ttgtcctaaa
    19561 gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa
    19621 atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca gcggagttat
    19681 gcgccctgcc agcgggcaaa gagatcttca ggaagggtta ccgcaaactg gtcaagaaca
    19741 cgattaaccg tctgatttat cacatcatca agggattgtg ggcgatgata aaacgccgga
    19801 acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg cagatggcct
    19861 aagtgcagtg gcgtttcacg cacgcagagc accaacgggc gacgctcttt cagcaccaca
    19921 tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga cagggttttg
    19981 attgaacagg gtaaaatcac catccccaac gtctggaaag aaccggaaga aatgctggca
    20041 gcaatatcgc gcgcatcgtg tgtcacatca gctaacgcct gcacttcgcg cagggaaaaa
    20101 tccgtttcga gggataaggt ctggcgcgcc gcctggctca ttaccagatg cgtttcgata
    20161 tctgtgacat cgcgcagaac ctgtaataaa cgcacgccat aaatcgcgcc gctggcaccg
    20221 ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc aggcagactt
    20281 tgcaggattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctg agcgaaggca
    20341 tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc
    20401 gtcgtcatta cgtaaagtat cgaggaaatc gaggtagccc tgatcaacgt ctttggtgac
    20461 gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccgggtttt cagcgcgaac
    20521 ggcttcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg
    20581 aatttcatct acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcgatacc
    20641 gtaaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc
    20701 ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga tagagtcgtc
    20761 gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcgttcagtt tacggcgcac
    20821 ggacttacga cgcagctgct gacccggcat gatgaaggtg cggccaacat agcggttttt
    20881 aacgaagccc tggcggtacg gtttgcccag aatacgcgca atttccagcg cgatatcgca
    20941 ggaagtttcc gggatcggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat
    21001 tttctcgccc agcttcgtgc ccatattcac acgcgcgctg taaacggaaa ttttgtcgat
    21061 gaacgagtcc gggcgagcaa agtatacata ctcaaacagg cacggattgc tgaccggatt
    21121 gtcagcacac tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgccagg
    21181 ggcgacatca cgcaggaact caaagcccag cgtatcgagc gcaacgcttt cggaagcgac
    21241 catatattct gtgcgattat cgtcaatatc acgtttgccc agtacaagcg gacgaatccc
    21301 gttaggatcg cggaaagcaa ccataccgtg gccgataatc atcaccacac aggcatacgc
    21361 gccgcggatt aagcggtttg tagcagcgat ggcggcgaaa atgttgtcgg cttccagcgg
    21421 gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt ccgagtcgga
    21481 agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt
    21541 ggtcagattg ccgttgtggg caagcgtaat gccatacgga gagttaacgt aaaacggctg
    21601 cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt
    21661 gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt
    21721 ccgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg
    21781 atgctgaagc accgttaagg catcataaat cgactggtta accggcataa caccggcgat
    21841 accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc
    21901 tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact
    21961 gaactgcggg atcagctgtg atttgctcca gtcttcgctt ttcgacaccc cggtaaagga
    22021 gtcgagaaag aagagaatgg cagcaacaat caacacaccg cgcaaggcac cgaaacagac
    22081 gcccaacacc cgatcggtgc ctgacaaccc cgttttctcc accaactggc ctatcacgaa
    22141 gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg caatcccatt
    22201 tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact
    22261 ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg
    22321 gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac
    22381 catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa
    22441 agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat
    22501 attcaacgcc atcgactttt tatgcctttg cggcatcggg caatgcgtgt cggatgcggc
    22561 gtgaacgcct tatccgacct acggctctac ccctgcacag gcctgataag acgcactggc
    22621 gtcacatcag gcaacggctg tcggatgcag cgtgaacgcc ttatccgacc tacggttcta
    22681 cccctgcaca ggcctgataa gacgcgctgg cgtcgcatca ggcaacggct gtcggatgcg
    22741 gcgtgaacgc cttatccgac ctacggttct acccctgcac aggcctgata agatgcgcca
    22801 gcgtcgcatc aggcaaaacc gtattaattc ggtgtatagc ccattaccac accacttaag
    22861 ccagaaagct gcttgagctc acccagcgat cctttcagct tctctttgga agcatctggc
    22921 ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga
    22981 taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgcg
    23041 cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc
    23101 ggttctggct tcggcgcagg tggtgcttca accttcggtt taggcgactc caccggtttc
    23161 ggcttcggtg ggacgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata
    23221 gtggccggat cgagcgacgg tgctgcggca tcgcctgccc gcacctcttc cgctgcgcct
    23281 tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga
    23341 tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc
    23401 ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact
    23461 aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg
    23521 tgcgaccgtg tggaacgaac cacacaccag cacggtgtct tccgctttag cgtccgccat
    23581 tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag
    23641 cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac
    23701 cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac
    23761 cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc
    23821 atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg
    23881 tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga cctccagccc
    23941 gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg
    24001 cagattttcc agcgtgccgt gagcatcgct aaacgtccag tcatgatcgg tgacggaata
    24061 gttccactca acgccccgac gttgtagcag tgcacctttt tcctgcccca catcagcaat
    24121 ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgaa agatacctgc
    24181 tttctcgcga ccaatacttt cgcgatctgg ccccagccag tcggtatgat ccagcgcaat
    24241 actggttact accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag
    24301 ccctacttcc agaatcacta cgtcaagttg tgcctgcttg aacagccaca atgccgacag
    24361 cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa
    24421 agaagcggtg tgggccgatt ccggcaattc ctgcccctgc acacgtacgc gctcggtata
    24481 acgcaccaga tgcggcgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga
    24541 ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg
    24601 cgctggtttc agaacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc
    24661 gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc
    24721 ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa
    24781 gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac
    24841 cggtggcacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa
    24901 cttcgccaga atgctcgcca gtttcagacg catttccgga cgacggacga tcatgtcgat
    24961 cgcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac
    25021 agtctgttcg ataacacgcg gaccggcaaa gccaattaac gctttcggtt cagcgatgtt
    25081 gagatcgccc agcatggcga aacttgcaga aacaccgccc atggtcgggt cggtcagcac
    25141 ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat
    25201 ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat
    25261 cagtgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgcg cacctaccac
    25321 agaccccatg gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc
    25381 atacagagtg cctttcatca ccaccagcgc atctttttcg ccggtttctt tctgcgcaga
    25441 tgccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag
    25501 ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc
    25561 tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc
    25621 gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc
    25681 cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc
    25741 tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc
    25801 ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag
    25861 tggtcgaacc gcagagttac tttttatttt gcggcgcgtg ccgctgcgcg tttgtggcga
    25921 ataaattcga aaacacctgg caatattgaa acaaaaataa tgccaacaat catcagttta
    25981 aggttgctct gaatgaaggg gagcgtgccg aagaaatagc ctgcgtaggt aaaaagcagt
    26041 acccacaaca gtgcgccaat cacgttataa gcggcgaaat gacggtacga catgtggccc
    26101 attcccgcaa caaacggggc gaacgttctg acgatcggca caaaacgggc gagaataatc
    26161 gttttgccgc catgtttctc ataaaactga tgggttttgt cgagataact gcgacggaaa
    26221 attttcgaat tgggattact gaataacttt tcaccgaaca accgcccgat agtgtaattg
    26281 accgcgtcac ccacaatcgc ggcgatcagc atcagcacca ccatcatatg gacgttgaga
    26341 tcgttggtct ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc acccggtaaa
    26401 aacggcgtta ccaccagacc ggtttcacag aacaaaatta agaacaaaat ggcataaacc
    26461 cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg
    26521 atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc tatcaggctg
    26581 taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa
    26641 tagcgggccc attggcggtt ttggaagatc atatcggtca gggtaatcca ccgcgaccag
    26701 atatagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa
    26761 ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat
    26821 attcctgacc atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc
    26881 gtgacgcgtg acattaatat gcataacgtt gcgccacggg gtccgggact ggcactgcac
    26941 cgcacggaac gaggtgaaat cattctcgcc cagcaaacat tgcgcagccc gatgcatccg
    27001 ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag
    27061 ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa
    27121 atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac
    27181 gcccaacgtc cacgcagcgt ctttgcgctg cgcggtcgtt tcgaaatgca caacctgccc
    27241 ggtaccgtgt acaccggcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc
    27301 cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg
    27361 ccagccgtaa tacctactgc cgtcgtactc aatgcccagc gcaattttat aaactggcgg
    27421 ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc aatttttact
    27481 gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg aacttgctcc
    27541 ggcataccgt agtcattacg tacgcagcca acagaaagat gcggcgtacc agaagcatcc
    27601 cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac
    27661 gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca
    27721 tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc
    27781 aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc
    27841 ggcagtaacg gcagcatgtt gaacgccagc tgacgcccga agaaatcttc ttcgtcaatc
    27901 ggaatgccat tgagtaattt agcactctgc cccgctaacg catcgaccgc ttttttgccc
    27961 tgggcggagg ctgaaatcag gctggtaacg ctgatacgtg ataaaccgcc ctgatcaatt
    28021 agcggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc
    28081 cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg
    28141 ggttcgagag caaacaatcc gctgctgtcg atcaccagac aacctgagtt ggtcgcttct
    28201 tcaacccagg cagcggtagc ctctttgcct gcgacaaaaa atgccagctg cgcctgcgtc
    28261 cagtcgaatt cagcagcatc ctgcacggtg attgtcttac caccaaagcg cagttgttcg
    28321 cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc
    28381 agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag
    28441 ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaata ttacagcaga
    28501 gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca
    28561 gcaatgatgc cgcgctggcg tcatcacaaa tcacatacag agatgaccat tcacggcgct
    28621 caagatagtt tttgcgcagc ttatcgaact cacccggtat tccggcgact ttacgcagcg
    28681 gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag cctttttagc gtcggttgat
    28741 cgagcggacc atgcagcgta atgcggccaa actctggcgc gggcagcaac gtatccagcg
    28801 caacgtgttg ttcatgccca ataagcttgc tataggcttc aaacacttgc gtagtaccgc
    28861 gtgctttacc ttccagggta tagcctgcga tatgcggtgt gccgatatcc acttttttca
    28921 gcaattccac gttgagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct
    28981 ggccttcgtt cagacaggtc aatagggcag tattatcgac gactgcgcca cggcaggcgt
    29041 taatcagaat cgctcctggt ttcaggctac ggatcagttt ttcatccgcc agatgcagcg
    29101 ttttgtacgg cccgtctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa
    29161 ctaattcatc cagcgagcgg aaatccccct catccccgcg gtcggcgcga ggcggatcgc
    29221 aaagcaaggt tgtaatccct aacgcttcca gtcgcgcctg taaacggcga ccaacgttac
    29281 caacgcccac gatccccact gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca
    29341 tcagcagtga ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg
    29401 aaaaaccaat tcccgcctgc tttaaccatg cttcatcgac atggtcggtc cccgcggtgg
    29461 cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg
    29521 aacgcaccat aagcgcatcc gcgtctgcca gttgagcgac aggaattgga cgcccgggaa
    29581 ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa
    29641 caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccagac aagtgtgcca
    29701 taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa
    29761 cctatttccg gccctcctcc tgggcaacca ccaggccagg gagataacct gccgtctggc
    29821 gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa
    29881 gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag gcacgatatt
    29941 ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcacaatctg
    30001 gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta
    30061 ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac
    30121 tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaagaccat tctcaccctg
    30181 ttgcaggaag ggaagatggt tattccgcaa cctcagcagc gcgaggcgac cgaccgtccg
    30241 ttattaccgg cggagcacaa tgcgctcaaa cagctggtga ccaaacttgc ggcggcaacg
    30301 ggagaaccca gcaagcagat ctggcaatcg atgctggaac tttccggggt gaaagatggc
    30361 gagttaattc cagcgaaact gtttaaccat ctggtgacct ggctacaggc gcgtcagacg
    30421 ctaagccagc aaaatacgcc gacgctggaa tcactccaga tggcgctaaa acaaccttta
    30481 gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg
    30541 caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg
    30601 gttaaaggga ttgagccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg
    30661 gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc
    30721 ctgatgctgg tctggctggt tcgttagccc cgacgaaatg acagtatcgt gacaataatt
    30781 cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc
    30841 agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg
    30901 cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca
    30961 ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca
    31021 ccaggggctt gccagagcag caacaagccc accgtttcta caagcagaga gacgatcgcc
    31081 actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat
    31141 gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttact ggcaaagtag
    31201 agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag acctaaccct
    31261 ggtttccaga taagcccgac aacgctccac agcgatggac gttctcccgc cagggccggt
    31321 actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaatgccat tgtggtgagc
    31381 gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggcgc accaacagcg
    31441 agggcaccgt aaatcgccat tccgttccat gacatcactt tgccggaatg ttttggccca
    31501 acgatgccta atccccaggt cagagcgccc gtcagtaact ggctttcacc aaagccaaga
    31561 atcaaacgcc cgacgaccaa cagggcaaat ttgaacggtg cggagacggg caaaatcgcc
    31621 gccaacagca gcgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt
    31681 tttgcgccat attgatcggc cagtcgcccg gcgtaaccac gcgtcagcac cgtagccaga
    31741 aactgaatgc cgacggcaat accgaccatg gtgttgccat agcccagttc atgatgaaca
    31801 aacagcggga taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa
    31861 gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc
    31921 atgcattact ccagaatgca gcgcaaggcg aggaatttcc ccgtctcatc tctctggttt
    31981 cagggttacg gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa
    32041 agcgggaagt ttgcctggaa caggcggcga ttgtcaataa tgtgaaaaag ggaaccatca
    32101 ggttcccttt tgcgttagtg tcggatgccg cattggcgcg taatgtcgga tgcgacgctg
    32161 gcgcgtctta tccgacctac tccgaattaa tctttcagct tgcgcattac cagcgtggcg
    32221 ttggtgccgc cgaagccgaa gctgttagac ataactgtgg tcagttcgcg atcggtcgtt
    32281 tcggtcacga tgttcagacc cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg
    32341 gcgataaaac cgtgttccag catcagcaga gagtagatag cttcctgtac gccagcagcg
    32401 cccagagagt gaccggtcat tgctttggtt gcagaaatcg ccgggctctt atcgccgaac
    32461 acttcacgga tagctgccag ttctttcacg tcgccaaccg gagtcgaagt accatgagag
    32521 ttcaggtagt cgattggggt atcaacaccg tgcatcgcca tcttcatgca gcgcactgca
    32581 ccttcgccag acggagcaac catgtctgcg ccatcagagg ttgcgccgta gccaacgatt
    32641 tcagcataga tgtgagcacc acgcgccagc gcgtgttcca gctcttcaac cactaccata
    32701 ccgccaccgc cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt
    32761 tccggggtgt cgttgtattt agtagacagc gcacccatcg cgtcgaattc gcaagccatt
    32821 tcccagcaca gctcttcgcc gccgccagca aacacgatgt cctgtttgcc cagttggatc
    32881 tgctctactg cgttaccgat acagtgtgcg gaagtcgcac acgcggagct gatggagtag
    32941 ttaacgccgt gaattttaaa cggcgtggcg aggcaggcag aaacgccgga tgccatcgct
    33001 ttggtgacca catacgggcc aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg
    33061 ccgaaaacct ggaaacgcgg ggagccgccg ccggaacctg caatcaggcc aacgcgcggg
    33121 ttattctggt aagcttccgg ggagaggcca gcatcagcaa ttgcctgctc catagaaagg
    33181 aatgcataaa tggatgcgtc gctcataaag cgcacaactt tgcggtcaat gaggccagtg
    33241 gtatccagtt ttacgttgcc ccagacgtgg ctacgcatgc cggaatcctt cagctcctga
    33301 gagaaagtga tccctgaacg tccttcacgc agagatgcca ggacttcctg ctggttatta
    33361 ccgatgctgg aaacaatgcc caggccagta atcactgcac gtttcattca atacctctgt
    33421 aagtcgcaca tagagtaagt ttcgaatgca caatagcgta cacttgtacg ccgaacaagt
    33481 ccgatcagcc aattaataga gaaatttgcg cagcctgaca cacatcgcta agatcgagcc
    33541 accgcctgta agacgagtaa cttacgtgaa acactactcc atacaacctg ccaacctcga
    33601 atttaatgct gagggtacac ctgtttcccg agattttgac gatgtctatt tttccaacga
    33661 taacggactg gaagagacgc gttatgtttt tctcgggggt aaccatttag aggcacgttt
    33721 tcctgaacat ccacatcctc tgtttgtggt agcagagagc ggcttcggca ccggattaaa
    33781 cttcctgacg ctatggcagg catttgatca gtttcgcgaa gcatatccgc aagcgcaatt
    33841 acaacgctta catttcatta gttttgagaa atttcccctc gcccgtgcgg atttagcgct
    33901 ggcgcatcaa cactggccgg agctggcccc gtgggcggaa cagcttcagg cgcaatggcc
    33961 tttgcccttg cccggttgcc atcgtttatt gctcgatgaa ggccgcataa cgctggattt
    34021 atggtttggc gatataaacg aactgaccag ccaactggac gattcgctaa atcaaaaagt
    34081 agatgcctgg tttctggacg gctttgcgcc agcgaaaaac ccggatatgt ggacgcaaaa
    34141 tctgtttaac gccatggccc ggctggcgcg tccgggcggc acgctggcga cctttacgtc
    34201 tgccggtttt gtccgccgcg gtttgcagga agccggattc actatgcaaa aacgtaaggg
    34261 ctttgggcgc aaacgggaaa tgctttgtgg ggtgatggaa ctgacattac ccctcccctg
    34321 ctccacgccg tggtttaacc gtacgggcag cagcaaacgg gaagcggcga ttatcggcgg
    34381 tggtattgcc agcgcgttgt tgtcgctggc gctattgcgt cgcggctggc aggtaacact
    34441 ttattgcgcg gatgaggccc ccgcactggg tgcttccggc aatcgccagg gggcgctgta
    34501 tccgctttta agtaaacacg atgaagcgct aaaccgtttt ttctctaatg cgtttacttt
    34561 tgctcgtcgg ttttacgatt tattgcccgt taaatttgat catgactggt gcggcgtcac
    34621 gcagttaggc tgggatgaga aaagccagca taaaatcgca cagatgttgt cgatggattt
    34681 acccacagaa ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc
    34741 gacaaattgc agcggtatta cttatccgca aggtggctgg ctgtgtccag cagaactgac
    34801 ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag attcattatc aatatcagtt
    34861 acaggattta tcccgtaagg atgacggttg gttgttgaat tttgcaggag atcagcaagc
    34921 aacacacagt ttagtggtac tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc
    34981 atctctgcca gtatattcgg tggccggtca agtcagccat attccgacaa cgccggagct
    35041 ggcgaaactg aagcaagtgc tgtgctatga cggttatctc acgccgcaaa atccggcgaa
    35101 tcagcaccat tgtattggtg ccagttatca tcgcggcagc gaagagacgg cgtacagtga
    35161 tgaagatcag cagcagaatc gccagcggtt gattgattgt ttcccgcagg tacagtgggc
    35221 aaaagaggtt gatgtcagtg gtaaagaggc gcgctgcggt gtgcgttgtg ctactcgcga
    35281 tcatctgcca atggtaggca acgtgcctga ttatgacgct acactcgtgg aatatgcgtc
    35341 gctggcggag aagaaagatg aagcggtaag cgcgccggtt tatgacgatc tctttatgtt
    35401 tgctgcttta ggttcgcgcg gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc
    35461 acagatgagt gaagaaccga ttccgatgga tgccagcacg ctggcggcgt taaacccgaa
    35521 tcggttatgg gtgcggaagt tgttgaaggg taaagcggtt aaggcggggt aatctgctct
    35581 ggcattgttt gtcggatgcg gcgtaaacgc cttatccgac ctacgcgtta cctgtaggcc
    35641 tgataagacg cgataagcgt cgcatcaggc atggtgctcc agacgtcaca acttactgct
    35701 gtgaggcctg ctgaaacagg tgttcccaca tatcggtcac cagcgcctgg tcacgcggcg
    35761 acaattcacc ggcaccaatg gctttttcca gactctggct aacggttgta tgtaccgcct
    35821 gagcggagtg gtcgtcacca ctttccagtt ctgcgatggc taatgtcagg tggccacgca
    35881 aatacccact ggcaaacaac tcatcatcac tggcatggtc aaccataccg tcgattaatg
    35941 ccagaatgcg tgattcaaac tccgcgatca tcttctttcc tcattgtaaa acgtggcgct
    36001 ggcttcagtt gagcgcttcc ggccacggga actgttccgc cgtaagttcc ggcgtgtgat
    36061 aataattttg tagtgcttta atgaagcgtg ccggacgttc gggaatgccg tttgccagat
    36121 aatccatcac ctgcgcatgt acccgccgtt gaaacaccac gcggtccggt tcgaaatccc
    36181 cttccagatt gtcgcagcta acattaaacg gatatcccgc tgccacgcag aacaaccagt
    36241 ccagcgcctg cggcttcact tcaacatctt caaactggct ttgtgtctgg gcatcgcgtc
    36301 cgtccgggca ataccagtag ccgaaatcaa ccagctcacg gcgcgctttc ccggcaatac
    36361 accagtgcga aatctcgtgg atggcgctgg cataaaagcc atgagcgaag acgattctgt
    36421 tatacggtac ttccgcatca gcaggaagat agatcggttc gtcgtcgcct ttaatcagac
    36481 gggtattaaa ttcatcggca aagcagctat taaaaatctc aatcaactgc tcatagtggt
    36541 gtgtactgtt cattagttca tccccaacca gtggaggatc tcctgtccat ggctatcgta
    36601 aagtaatttg gcactcatca ccgccgagac gataacgatc atcgggcgga tcagcttttg
    36661 tcctttgctc aacaccagtc ttgaacccat gcgcgcgccg aggaactgcc ccaccagcat
    36721 cacaaagccc gtcgcccaaa tcactttgcc gccgagaata aacagcagca gaccgccaat
    36781 gtttgacgtt gcgttaagta atttggcgtg agcagtggct ttggcgaggt tgaatccgca
    36841 cagcgtaacg aaggccagcg cgtaaaacga tccggcagcc gggccaaaaa atccatcgta
    36901 aaaaccgacg cagccccccg ccaccagcgc aaatggcagt ccatacatcc gacgctggcg
    36961 atcttcttca cccagctttg gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat
    37021 aggcaaaatc tgccgtaaga catcggcctg aacatactgc accagtagcg cgccgctcat
    37081 tgagccgaca aaggtcatgg cgatattgag tttttgatcg cttaaactaa ccactttacg
    37141 gcgaataaag tagatagtgg cggaaataga gccaccgcag gcttgcagtt tattggttgc
    37201 cagcgcgtta gcgggagaca tccccgccgc catcaatgcc ggaatggtga gtaacccacc
    37261 gccaccggca atcgagtcga taaatcccgc cagcatggcg acaaaaaaga gaactcccag
    37321 cagcagcggg gaaaccataa acaggctatt aaacgtttcc attagatcac gtgctcatcc
    37381 agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt
    37441 cccggttttg gaggttcaaa ccagctttgc agttctgccc cgcaaccatc gcctggtggc
    37501 ggtaaaggtt gatcctcaca ctccagacta tcggcaggac aacgtaatcg tacatgcata
    37561 tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca
    37621 tcaaggcaaa gttgttgctt aatcgccgga ttaacaaaaa tgcgcgtgac gtctttatcc
    37681 tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc acagcgtcgg gacaacgtgt
    37741 ttaccgtcgc gggaaactaa atctaacgct tgcgggcgca agagttgcgc ggaggtccag
    37801 cgtgttttcg gcagttgcag gaagatatcg acatccagtc cggtctggtg gctggcgtga
    37861 ccgccgttga aacgaccacc agcaggcatc cccatatcgc caatcagcac cgtacccatg
    37921 cccagattgc tcacctggcg actcagacgc tggataaaca tcaccaggtc tgggtgaccg
    37981 aaatagcgac gctgatcggt acgcatgacc tgataatgtt cggactgtat cggcaacgtg
    38041 tcagcaccga tgatacagcc attagaaaaa ctgcctatcg attgtgcgct acccggcaca
    38101 ggttgggtta ttttttgcca cggcgtcgct gccaggctgg cgctactggc aagcagagcc
    38161 agcagcgcaa tcgcggtttt gttcattttt taccagcgtg gaatttcagt cttcacatcg
    38221 gcattttgcg cccgctgccg taacaggtga tccatcaaaa cgatcgccag catcgcttct
    38281 gcgatcggca ccgcgcggat cccaacacaa ggatcgtgac ggcctttggt gatcatctca
    38341 acttcttcgc caaagcggtt aatagtgcgc cccggcacgg taatgctgga ggtcggtttc
    38401 agcgccatgt gggcaatgat ttgctgcccg ctgctgatac caccaagaat gccgcccgca
    38461 tggttgctct ggaaaccgtc tttggtgatt tcgtcgcggt tctggctgcc acgcagcgct
    38521 accacgtcaa aaccatcacc aatttccacg cctttcaccg cgttaatgct cattagcgca
    38581 tgggcgatgt cggcatccag gcggtcaaag accggctcgc caagtccggc gggtacgcca
    38641 ctggcaacga cggtgacttt cgcaccaata gagtcgcctt cttttttcag cgcgcgcatc
    38701 agttcatcta acgcgtcgat tttgtccggg tccgggcaga agaatggatt ttgctcgacc
    38761 tgagaccagt ctttgatttc cagcggaatg tcgcccatct gggtcaggca accgcgaatc
    38821 tcaatgccaa atttctcggc gagatatttt ttggcaatcg cccctgctgc aacgcgcata
    38881 gcggtttcac gggcggaaga acgtccaccg ccgcgataat cacgcaggcc gtatttttgt
    38941 tcgtaagtgt aatcggcatg acctggacgg aaaacgtcct taatcgcact ataatcctga
    39001 gaacgctggt cggtattttc gatcaacaat ccgatgctgg tgccagtagt aacaccttca
    39061 aaaacaccgg agagaatttt gacctgatcc ggctcgcggc gctgggtggt atagcgcgat
    39121 gtcccagggc gacgacgatc aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct
    39181 ggcggaacac catcgacgat gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt
    39241 acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg
    39301 cgtgtttact taatctttat aaatcgcgaa atgttctcgt gcggcaataa gctgctcttt
    39361 agtgagcata aacacaccat cgccgccgtt atcaaactcc agccaggtga acggaacatc
    39421 cggatattgt tccataagat gtaccatgct gttgccgact tcacaaatca gcacgccatc
    39481 atcagcgagg taatctgccg cgtttccgag aatgcgacgc gtcagtttca ggccgtcggt
    39541 gccagatgcc aggcccagtt ccggctcgtg gcgatattcg tttggcaggt cggacatatc
    39601 ttccgcatcg acatacggcg ggttagtgac aatcaggtcg tactgcactt tcggcaagtc
    39661 gcggaacaaa tcggaccgaa taggaatgac gttgtggatc agaccgtgtt cttcgatgtt
    39721 ctgttcagca accgccagcg cgtcgggaga gatgtccacc gcgtcgactt ccgcttccgg
    39781 gaaggcataa gcacaggcaa tggcgatgca gccgctaccg gtacacatat ctaaaatgtg
    39841 ctgcggttgc ttgctgatga gtccggcaaa tttattgttg atcagttcac caatcggcga
    39901 gcgcggcacc agcacgcgtt catcgacgta aaattcatgg ccgcagaacc aggctctgtt
    39961 ggtcaggtaa gccaccggaa tgcgttcgtt gacgcggcgg atcacgcgct caacaatacg
    40021 gtgtttttcg ctggaggtca gacgcgcggt gcgcatgtct tccggaatat ccagcggcag
    40081 gtagagtgaa ggcaacacca gttgcacggc ttcatcccac gggttatcgg tgccgtggcc
    40141 gtaccagata ttcgccgcgc tgaagcggct caccgaccag cgcaacatgt cctgaatggt
    40201 ttgcagctca tttactgctt catcaacgaa aattttatcc acgtattcct ccagggcatg
    40261 atcgcaataa tttcggcggc tagtttgcca tgaagatgac gataaatcag cattcacgcg
    40321 cggtgagtga ggaaaaatac gtttaaaacg atcgattgcg ctac
//
LOCUS       DAPQZD010000022        40300 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPQZD010000022 DAPQZD010000000
VERSION     DAPQZD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.022"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..624
                     /gene="nfeR"
                     /locus_tag="RO454_001168"
                     /gene_synonym="yqjI"
     CDS             1..624
                     /gene="nfeR"
                     /locus_tag="RO454_001168"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDY0933035.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLCVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(731..2251)
                     /gene="aer"
                     /locus_tag="RO454_001169"
     CDS             complement(731..2251)
                     /gene="aer"
                     /locus_tag="RO454_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDY0933036.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            2669..4048
                     /gene="ygjG"
                     /locus_tag="RO454_001170"
     CDS             2669..4048
                     /gene="ygjG"
                     /locus_tag="RO454_001170"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDY0933037.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4090..4422)
                     /locus_tag="RO454_001171"
     CDS             complement(4090..4422)
                     /locus_tag="RO454_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDY0933038.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4641..5624
                     /gene="ebgR"
                     /locus_tag="RO454_001172"
     CDS             4641..5624
                     /gene="ebgR"
                     /locus_tag="RO454_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDY0933039.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5808..8900
                     /gene="ebgA"
                     /locus_tag="RO454_001173"
     CDS             5808..8900
                     /gene="ebgA"
                     /locus_tag="RO454_001173"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDY0933040.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
     gene            8897..9346
                     /locus_tag="RO454_001174"
     CDS             8897..9346
                     /locus_tag="RO454_001174"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDY0933041.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9409..10842
                     /locus_tag="RO454_001175"
     CDS             9409..10842
                     /locus_tag="RO454_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDY0933042.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            10976..12046
                     /gene="ygjJ"
                     /locus_tag="RO454_001176"
     CDS             10976..12046
                     /gene="ygjJ"
                     /locus_tag="RO454_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDY0933043.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            12063..14414
                     /gene="ygjK"
                     /locus_tag="RO454_001177"
     CDS             12063..14414
                     /gene="ygjK"
                     /locus_tag="RO454_001177"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDY0933044.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPESWSPLFNGEATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            14640..16658
                     /gene="fadH"
                     /locus_tag="RO454_001178"
     CDS             14640..16658
                     /gene="fadH"
                     /locus_tag="RO454_001178"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDY0933045.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16703..17119)
                     /gene="higA"
                     /locus_tag="RO454_001179"
     CDS             complement(16703..17119)
                     /gene="higA"
                     /locus_tag="RO454_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDY0933046.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17116..17430)
                     /gene="higB"
                     /locus_tag="RO454_001180"
     CDS             complement(17116..17430)
                     /gene="higB"
                     /locus_tag="RO454_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HDY0933047.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(17715..18851)
                     /gene="rlmG"
                     /locus_tag="RO454_001181"
     CDS             complement(17715..18851)
                     /gene="rlmG"
                     /locus_tag="RO454_001181"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDY0933048.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18936..19439
                     /locus_tag="RO454_001182"
     CDS             18936..19439
                     /locus_tag="RO454_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDY0933049.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            19516..20208
                     /locus_tag="RO454_001183"
     CDS             19516..20208
                     /locus_tag="RO454_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDY0933050.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            20287..21273
                     /locus_tag="RO454_001184"
     CDS             20287..21273
                     /locus_tag="RO454_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDY0933051.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            21556..22521
                     /gene="alx"
                     /locus_tag="RO454_001185"
     CDS             21556..22521
                     /gene="alx"
                     /locus_tag="RO454_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDY0933052.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            22845..24089
                     /gene="sstT"
                     /locus_tag="RO454_001186"
     CDS             22845..24089
                     /gene="sstT"
                     /locus_tag="RO454_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDY0933053.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(24093..24644)
                     /locus_tag="RO454_001187"
     CDS             complement(24093..24644)
                     /locus_tag="RO454_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDY0933054.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(24727..26214)
                     /gene="uxaA"
                     /locus_tag="RO454_001188"
     CDS             complement(24727..26214)
                     /gene="uxaA"
                     /locus_tag="RO454_001188"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDY0933055.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(26229..27641)
                     /gene="uxaC"
                     /locus_tag="RO454_001189"
     CDS             complement(26229..27641)
                     /gene="uxaC"
                     /locus_tag="RO454_001189"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDY0933056.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            28124..29422
                     /gene="exuT"
                     /locus_tag="RO454_001190"
     CDS             28124..29422
                     /gene="exuT"
                     /locus_tag="RO454_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDY0933057.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            29552..30328
                     /gene="exuR"
                     /locus_tag="RO454_001191"
     CDS             29552..30328
                     /gene="exuR"
                     /locus_tag="RO454_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDY0933058.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            30673..31335
                     /locus_tag="RO454_001192"
     CDS             30673..31335
                     /locus_tag="RO454_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0933059.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKFEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            31339..31722
                     /gene="mzrA"
                     /locus_tag="RO454_001193"
     CDS             31339..31722
                     /gene="mzrA"
                     /locus_tag="RO454_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDY0933060.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            31869..32237
                     /locus_tag="RO454_001194"
     CDS             31869..32237
                     /locus_tag="RO454_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDY0933061.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            32275..32580
                     /locus_tag="RO454_001195"
     CDS             32275..32580
                     /locus_tag="RO454_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDY0933062.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            32583..32987
                     /locus_tag="RO454_001196"
     CDS             32583..32987
                     /locus_tag="RO454_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDY0933063.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            32977..33276
                     /locus_tag="RO454_001197"
     CDS             32977..33276
                     /locus_tag="RO454_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDY0933064.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            33463..33855
                     /locus_tag="RO454_001198"
     CDS             33463..33855
                     /locus_tag="RO454_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDY0933065.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            33925..34911
                     /gene="yqjG"
                     /locus_tag="RO454_001199"
     CDS             33925..34911
                     /gene="yqjG"
                     /locus_tag="RO454_001199"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDY0933066.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            35194..35562
                     /locus_tag="RO454_001200"
     CDS             35194..35562
                     /locus_tag="RO454_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDY0933067.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(35596..36492)
                     /gene="yhaJ"
                     /locus_tag="RO454_001201"
     CDS             complement(35596..36492)
                     /gene="yhaJ"
                     /locus_tag="RO454_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDY0933068.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            36597..37298
                     /locus_tag="RO454_001202"
     CDS             36597..37298
                     /locus_tag="RO454_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDY0933069.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            37321..37485
                     /locus_tag="RO454_001203"
     CDS             37321..37485
                     /locus_tag="RO454_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933070.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(37546..38856)
                     /locus_tag="RO454_001204"
     CDS             complement(37546..38856)
                     /locus_tag="RO454_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDY0933071.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(38884..39762)
                     /locus_tag="RO454_001205"
                     /pseudo
     CDS             complement(38884..39762)
                     /locus_tag="RO454_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein"
     gene            39835..40300
                     /locus_tag="RO454_001206"
                     /pseudo
     CDS             39835..40300
                     /locus_tag="RO454_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
BASE COUNT         9618 a        10574 c        10405 g         9703 t
ORIGIN      
        1 atgagccatc atcacgaagg gtgttgtaaa catgaaggcc agccacgcca tgagggctgc
       61 tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt
      121 caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac
      181 ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgag
      241 ttgattaaag cgattgaaaa tctgacccag gggaattaca ccccaagccc gggcgtcatc
      301 tacccgacgc tggattttct gcaggagcag tcgctgatta ccatccgcga agaggaaggt
      361 ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa
      421 caggtagaga tgattgaaga acgcatcaaa gcgcgttgcg ttggcgctgc gctgcgccag
      481 aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tgctggattt atgcgtcaac
      541 cagagcgata tcactgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct
      601 tttgatatta cccaactgga ttaatcaccg attgccggat gcggcgtacc ctacacaccc
      661 cgtaggcctg ataagatgcg ccagcatcgc atcaggcatt gtgctccaac cgccggatcc
      721 ggcataccga ttaatgcagc accgtcaccg cgtcttccag tcggctggcg cggtgtttca
      781 ccatcgccga cgcctgcgca ctctcttcca ctaactcggc gtttttctgg gtgatcaggt
      841 taagctcatc cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc agcgttgaat
      901 ggctaatctg agctatcaac tgggtgacgt tttttacctg cgccacaata tcttccatcg
      961 tccgtccggc ggcgtgtacc tgctgcgaac cagattgcac cttatcagca ctggcatcaa
     1021 tcagcttgcg aatatcgttg gcagcattgg cgctgcggct ggctaaatga cgcacttccc
     1081 ccgccaccac tgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc accgccgcat
     1141 tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta atggtgccaa
     1201 tgcgctgggt actgtcggca atatcgtcca ttgtcttgat caccgtagtc atcgcctccc
     1261 cgccctgcac tgccgcatta ctggcggtga tagaaagttt atcggcagcc gacgccgtgg
     1321 cagagttctg tttcaccgac gccgccattt ggttcatggt cgccaccgtt tgctgaacgt
     1381 tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc agcgtctcac
     1441 tgccgtttct gacactggac acctggcttg agacatcatt aattagccaa cggcacatca
     1501 ggccaagctg ccctaccgcg cgtaatgtca gccccagctc atcgctgcgc ttcagatgct
     1561 caacactatt acgttctccg gtcgccactt tcagtgcctg gctggcgaca ttttctatcg
     1621 gacgtacaat ttgccattca aaacaggcat ttcccaacaa catcactaat gcacagaaga
     1681 tatacgtcac caccggggca gcaacaaacc agagcatggc cgccagcacg ataaacatca
     1741 gggtcatcac tccacgcgcc cgccagcgaa gcggtaatga aggcagttta cccagccagc
     1801 ctttacgcac caccaggcct ttatgaatac gcttaccggt acgtccggca ttcagcgctt
     1861 tgtacagcgg ctccaccgcc gcgatctctt catccgtcgc ccgggtacga atcgacatat
     1921 agccactgat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc caataatggt
     1981 cgccattttt gcggcgattt ttcacgatgc cgctccaggg ttcccctttt ttcagggtga
     2041 accacatatc cgcaaacgcc gcttttggca tatccgggtg acgcaccatg ttgtgcggtt
     2101 gcccctgtaa ctcctgcaag gtatagccgc tcacctgcac aaaagtgtca ttagcatgag
     2161 tgatatagct ttgcagatcg gtagtggaca tcagagtggt gtcgtccgcc agcggggtat
     2221 tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt tatatggttg
     2281 ttaacttctt gtcagagttt atgtcggccc cgctgcggtt atctttaacc aattaatttg
     2341 atttagatcg caatttgcga ttaaagcaca aatctaattc cttgatttaa aatactttca
     2401 ctctgttact atacgaaaac gttaattatc ttgcccaaaa atcaggcaat tattgccctg
     2461 aaagcgtgca tttgcgcagc aatcatcaaa tccatacccg acaaaaaccg tgcaaaataa
     2521 cagcaaatgt tagcaaatag cattaaatat tgcgcaaatg ataaccacat ttgtgtttat
     2581 cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa atcggtatca
     2641 tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg catcagcttt
     2701 agcgtgcagc gcccacgccc tgaatctcat tgaaaagcga acgttggatc atgaggagat
     2761 gaaagcactt aaccgggagg tgattgaata cttcaaagag catgtcaatc cggggttttt
     2821 agagtatcgc aaatctgtta ccgccggcgg ggattacgga gccgtagagt ggcaagcggg
     2881 aggtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc tgggaggttt
     2941 tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac agaatcaact
     3001 tgcgaaacaa ccgcttcaca gccaggagct gcttgatccg ttacgggcga tgctggcgaa
     3061 aacccttgcg gcgctaacgc ccggtaaact gaaatacagc ttcttctgta atagcggcac
     3121 cgaatccgtt gaagcggcgc tgaagctggc gaaggcttat cagtcaccgc gcggcaagtt
     3181 tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc tgtcggccac
     3241 ggcgaaatcg accttccgca aaccgtttat gccactgctg ccagggtttc gtcatgtacc
     3301 gtttggcaat atcgaagcca tgcgcacggc tcttaacgag tgcaaaaaaa ccggtgatga
     3361 tgttgctgcg gtgatcctcg aaccgattca gggcgaaggt ggcgtaattc tgccgccgcc
     3421 aggctatctc accgccgtgc gtaagctgtg cgatgagttc ggcgcactga tgatcctcga
     3481 tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgagc atgagaacgt
     3541 acagccggat atcctttgtc tggcaaaagc gctcggcggc ggcgtgatgc cgattggcgc
     3601 aaccatcgcc actgaagagg tgttctcagt cctgttcgac aatccgttcc tgcacactac
     3661 cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca atgtgttgct
     3721 ggagcagaac ttaccggctc aggctgaaca aaaaggcgat atgttgctgg acggtttccg
     3781 tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggtaaag ggatgttgat
     3841 ggcgattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga tgttccgcca
     3901 gcgcgtactg gtggccggaa cactcaacaa cgctaaaacg atccgcattg aaccgccact
     3961 gacactgacc attgaacagt gtgaactggt gatcaaagcg gcgcgtaagg cgctggcggc
     4021 catgcgagtc agtgtcgaag aagcgtaata tcaaatcgga tggcgatacg acgtcgccat
     4081 ccgatctttt tatacgatac gtacgcccgc aggcatcatc cgctccggcg ttaacagcac
     4141 gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg tttcaccgcg
     4201 cattttcgct ttttgcagat tgcataagac caccactgtt tttcccatca gctcttcttc
     4261 gctgtagtac ggcaccagac tggtcacggt ttgcagcgtt ttttccccca catcaacctg
     4321 tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg
     4381 catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgtttcct cccttcaagt
     4441 aaaattttac taaactatag caaaagtttt tctcaatcct gtaggctaaa aatggagaat
     4501 gcaggcgtga tcacattcgt gagccgctgt gttaccgtca cagcgtcaaa gaaacgcgct
     4561 ttatttactg aaaacaggtg acccgataag cacttcctct acaatggggg cgcacatcag
     4621 ggaaagtaaa aaaggtaaac atggcaacac taaaagacat cgcaatcgaa gctggcgtat
     4681 ccctggcgac agtatccagg gtcttgaatg acgatccgac attgaatgtg aaagaagaga
     4741 cgaaacatcg cattctcgag atcgccgaaa agctggagta caagaccagt agcgcccgta
     4801 aactccagac aggcgcagtc aaccaacacc atattctggc tatctacagc taccaacagg
     4861 agttggagat caacgatcct tactatctgg cgatccgcca cggcattgaa actcagtgcg
     4921 aaaagctggc gatcgaactg accaactgtt atgaacacaa cggcttacca gacattaaaa
     4981 acgtcaccgg tattttaatt gtcggcaaac ccacgtccgc cctgcgcgcc gccgcctgtg
     5041 cgttgaccga caatatctgt tttatcgact ttcacgaacc cggcagcggt tacgatgcgg
     5101 tggatatcga tctggcacgt atcagtaaag aaatcatcga tttctatatc aatcagggcg
     5161 ttaatcgtat tggttttatt ggcggtgaag acttacccgg caaggcggat attcgtgagg
     5221 tcgcctttgc ggaatatggt cgtctgaaac acgtggtacg cgaagaggat atctggcgcg
     5281 gcggtttttc cagttcgtcg ggctatgaac tggcaaaaca gatgctggcg cgggaagact
     5341 atccgaaggc actgtttgtt gcttccgatt ccattgctat cggcgtactg cgggcaattc
     5401 atgaacgtgg cctgaacatc ccacaggata tttcgcttat cagcgttaac gatatcccca
     5461 ccgcgcgatt tacctttccg ccgctctcca ccgtgcgcat ccattccgag atgatgggaa
     5521 gtcagggcgt taacctggtg tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgttgg
     5581 tcttcgttcc cagcaaatta aaactgcgcg gcacgacccg ttaaatcccc ttacacactg
     5641 tccggcaatc gtttttgccg gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa
     5701 atgcatttac ctgctatttt ttagtaaaaa ttttactaaa ctccccagca attacacaaa
     5761 ctaccatcac cataaatggt tccgatttct ctctaccggg aggccctatg aatcgctggg
     5821 aaaacattca gctcacccac gaaaaccgac ttgcgccgcg tgcgtacttt ttttcatatg
     5881 attctgttgc gcaagcgcgt acctttgccc gcgaaaccag cagcctgttt ctgtccttaa
     5941 gcggtcagtg gaatttccac ttttttgacc atccgctgca agtaccagaa gccttcacct
     6001 ctgagttaat ggctgactgg gggcatatta ccgtccccgc catgtggcaa atggaaggtc
     6061 acggcaaact gcaatatacc gacgaaggtt ttccgttccc catcgatgtg ccgtttgtcc
     6121 ccagcgataa cccaaccggt gcctatcaac gtattttcac cctcagcgac ggctggcagg
     6181 gtaaacagac gctgattaaa tttgacggcg tcgaaaccta ttttgaagtc tacgttaacg
     6241 gtcagtatgt ggggttcagc aaaggcagtc gcctgaccgc agagtttgac atcagcgcga
     6301 tggttaaaac cggcgacaac ctgttgtgtg tgcgcgtgat gcaatgggcg gactctacct
     6361 acgtggaaga ccaggatatg tggtggtcgg cggggatctt ccgcgatgtt tatctgatcg
     6421 gaaaacaact cacgcacatt aacgatttca ccgtgcgcac cgactttgac gaagcctatt
     6481 gcgatgccac actttcctgc gaagtggtgc tggaaaatct cgccgcctcc cctgtcgtca
     6541 cgacgctgga atataccctg tttgatggcg aacgcgtggt gcacagcagc gccattgatc
     6601 atttggcaat tgaaaaactg accagcgccc gctttgattt tactgtcgaa cagccgcagc
     6661 aatggtcagc agaatcccct tatctttacc atctggtcat gacgctgaaa gacgccgacg
     6721 gcaacgttct ggaagtggtg ccacaacgcg ttggcttccg tgatatcaaa gtgcgcgacg
     6781 gtctgttctg gatcaataac cgttatgtga tgctgcacgg cgtcaaccgt cacgacaacg
     6841 atcatcgcaa aggccgcgcc gttggaatgg atcgcgtcga gaaagatctc cagttgatga
     6901 agcagcacaa catcaactcc gtgcgtaccg ctcactaccc gaacgatccg cgtttttacg
     6961 aactgtgtga tatctacggc ctgtttgtga tggcggaaac cgacgtcgaa tcgcacggct
     7021 ttgctaatgt cggcgatatc agccgtatta ccgacgatcc gcagtgggaa aacgtctacg
     7081 tcgagcgcat tgttcgccat attcacgcgc agaaaaacca tccgtcgatc atcatctggt
     7141 cgctgggcaa tgaatccggc tatggctgta acatccgcgc gatgtaccat gcggctaagg
     7201 cgctggatga cacgcgactg gtgcactacg aagaagatcg cgatgctgaa gtggtcgata
     7261 ttatttccac catgtacacc cgcgtgccgc tgatgaatga gtttggtgaa tacccgcatc
     7321 cgaagccgcg catcatctgt gaatatgctc atgcgatggg gaacggaccg ggcgggctga
     7381 cggagtacca gaacgtcttc tataagcacg attgcattca gggacattat gtctgggaat
     7441 ggtgcgacca cgggatccag gcgcaggatg acaacggcaa tgtctggtat aaattcggcg
     7501 gcgactacgg cgactatccc aacaactata acttctgtct tgatggtttg atctattccg
     7561 atcagacgcc tgggccgggc ctgaaagagt acaaacaggt tatcgcgccg gtaaaaatcc
     7621 acgcgttgga tctgactcgc ggcgagttga aagtcgaaaa taaactgtgg tttaccacgc
     7681 tggatgacta caccctgcac gcagaggtgc gcgccgaagg tgaaacgctc gcgacgcagc
     7741 agattaaact gtgcgacgtt gcaccgaaca gcgaagcccc cttgcagatc acgctgccgc
     7801 aactggacgc tcgtgaagcg tttctcaaca ttacggtgac caaagattcc cgcacccgct
     7861 acagcgaagc cggacaccct atcgctactt atcagttccc gctgaaggaa aataccgcgc
     7921 agcctgtacc tttcgcgcca aataatgcgc gtccgctgac gctggaagac gatcgtttga
     7981 gctgcaccgt tcgcggctac aacttcgcaa tcaccttctc aaaaatgagt ggcaaaccga
     8041 catcctggca ggtgaatggc gagtcgctgc tgacccgcga gccaaagatc aacttcttca
     8101 agccaatgat cgacaaccac aagcaggagt acgaagggct gtggcagccg aatcatttgc
     8161 agatcatgca ggaacatctg cgcgactttg ccgtagaaca gagcgatggt gaagtgttga
     8221 tcatcagccg cacggttatt gccccaccgg tgtttgactt cgggatgcgc tgcacctaca
     8281 tctggcgcat cgctgccgat ggccaggtta acgtggcgct ttccggcgag cgttacggcg
     8341 actatccgca catcattccg tgcatcggtt tcaccatggg gattaacggc gaatacgatc
     8401 aggtggcgta ttacggtcgt ggaccgggcg aaaactacgc cgacagccag caggctaaca
     8461 tcatcgatat ctggcgcagc accgtcgatg ccatgttcga gaactatccc ttcccacaga
     8521 acaacggcaa ccgtcagcat gtccgctgga cggcactgac taaccgccac ggcaatggtc
     8581 tgctggttgt tccgcagcgc ccaattaact tcagtgcctg gcactacacc caggaaaaca
     8641 tccacgcagc ccagcactgt aacgagctgc agcgcagtga tgacatcacc ctgaatctcg
     8701 accaccagtt gcctggcctc ggctccaact cctggggcag cgaggtgctg gactcctggc
     8761 gcgtctggtt ccgtgacttc agctacggct ttacgttgct gccggtttct ggcggagaag
     8821 ctaccgcgca aagccaggcg tcgtatgagt tcggcgcagg gttcttttcc acgaatttgc
     8881 acagcgagaa taagcaatga ggatcatcga taacttagaa cagttccgcc agatttacgc
     8941 ctctggcaag aagtggcaac gctgcgttga agcgattgaa aatatcgaca acattcagcc
     9001 tggcgtcgcc cactccatcg gtgactcatt gacttaccgc gtggagacgg actccgcgac
     9061 cgatgcgcta tttaccgggc atcgacgcta ttttgaagtg cattactacc tgcaagggca
     9121 gcaaaaaatt gaatatgcgc cgaaagagac attacaggta gtggaatatt atcgtgatga
     9181 aactgaccgc gaatatttaa aaggctgcgg agaaaccgta gaggtccacg aagggcaaat
     9241 cgttatttgc gatatccatg aagcgtatcg gtttatctgc aataacgcgg tcaaaaaagt
     9301 ggttctcaaa gtcaccatcg aagatggtta tttccataac aaataaaaac tacggcggca
     9361 aaaggagttt gccgccaccg ctaccctact cattttcgga gatgtgttat gtctgatacc
     9421 aaacgtaata caatcggcaa attcggcttg ctctcgctga cttttgccgc cgtttacagc
     9481 tttaacaacg ttatcaataa taatattgag cttggactgg cctcggcacc gatgtttttc
     9541 ctcgcaacaa ttttttattt tattcccttc tgtctgatca tcgcagaatt tgtttcgtta
     9601 aataaaaact cggaagccgg tgtctacgcg tgggtaaaaa gttcgctggg cggacgttgg
     9661 gcatttatta ctgcctatac ctactggttc gtaaacctgt tctttttcac ctcattattg
     9721 ccgcgcgtta ttgcttacgc ctcgtatgca ttcctcggct acgaatatat tatgacgccg
     9781 gttgccacca ccattatcag tatggtgctg ttcgcctttt ccacctgggt ttctaccaac
     9841 ggggcgaaaa tgctggggcc aattacctcc gtcacttcaa cgctgatgct gctgttaacg
     9901 ctctcctaca ttttactggc aggtacggca ctggttggcg gcgtacagcc tgctgacccc
     9961 atcactgttg acgcgatgat cccaaacttt aactgggcgt tcctcggcgt taccacctgg
    10021 atctttatgg ctgcaggtgg cgcggagtcc gtcgcggtgt acgttaacga cgtcaaaggc
    10081 ggttcgaaat cgttcgttaa agtgatcatc ctcgccggga tttttattgg cgtactgtac
    10141 tccgtctcct cggtactgat taacgtcttc gtcagcagca aagagttgaa gtttaccggc
    10201 ggatcggtac aggtattcca cggcatggcg gcgtattttg gtctaccgga agcactgatg
    10261 aatcgctttg tcggtctggt gtcctttacc gcgatgttcg gttccctgct gatgtggacg
    10321 gcaacgccgg tgaaaatttt cttctccgaa attccggaag gcatctttgg taagaaaacc
    10381 gtcgaactga acgaaaacgg cgttccggcg cgcgcagcgt ggatccagtt cctgatcgtc
    10441 atcccgctga tgattatccc gatgctcggt tccaataccg tgcaggatct gatgaatact
    10501 attattaata tgactgctgc agcgtccatg cttccgccgt tattcatcat gctggcttac
    10561 ctgaatttac gcgccaaatt agatcacctg ccacgcgatt tccgtatggg ctcccgccgc
    10621 accggtatta tcgttgtttc aatgctgatt gcgatatttg cagtagggtt tgtcgcttcg
    10681 acattcccga ctggcgcgaa tattctgacc atcatttttt ataacgtcgg cggtattgtt
    10741 atcttcctcg gctttgcgtg gtggaaatac agtaaatata taaagggatt aacggctgaa
    10801 gagcgccata ttgaagcgac gccagccagc aatgttgatt aagcagaata ataaaaaatg
    10861 agcaggcatt ttccctcttc ctggatgggg agagggaaaa tagtttctgc ctttatattt
    10921 ttataattac aacgataaaa ggctgtactt tttctttagc tcatggatta acacaatgaa
    10981 attaatcact gcaccatgca gagcattact tgccctgcct ttttgctacg ctttttctgc
    11041 acttggcgaa gaagcacgtc cggcagaaca tgacgacaca aaaacccccg aaatgatctc
    11101 gacatcttct ccttcatttc gtttttacgg cgaattaggg gttggtgggt atatggattt
    11161 agagggtgag aataaacata aatacagcga cggcacctat attgaaggtg gcctggagat
    11221 gaagtacgac tcctggttcg gcctgattta cggcgaaggc tggaccgtgc aggccgacca
    11281 cgacggtaat gcctgggtgc ctgaccatag ctggggtggt ttcgaaggtg ggattaaccg
    11341 tttctatggc ggttatcgta ccaatgatgg caccgaaatc atgctcagtc tgcgtcagga
    11401 ttcctcgctg gatgacctgc aatggtgggg cgatttcacg cccgatctgg gctacgtcat
    11461 tcccaatacc cgcgacatta tgactgcgct gaaggtacag aacttaagcg gcaactttcg
    11521 ttatagcgtt accgccacac ctgccggaca tcatgacgaa agcaaagcct ggctacattt
    11581 tggcaagtac gatcgctacg acgacaaata cacctatccg gcaatgatga acggttacat
    11641 ccagtatgac cttgcagaag gtatcacctg gatgaacggt ctggaaatca ccgacggcac
    11701 aggccaactc tatctcacgg gcctgcttac ccctaacttt gccgctcgcg cctggcacca
    11761 taccggacgc gccgacgggc tggacgtacc gggaagtgaa agtgggatga tggtgagcgc
    11821 catgtatgaa gcgttaaagg gcgtttatct ctccaccgct tacacctacg ccaaacatcg
    11881 ccctgaccac gctgacgatg aaaccacctc tttcatgcag tttggtatct ggtacgaata
    11941 cggcggcgga cgtttcgcaa cggcttttga tagccgcttc tacatgaaaa atgcctctca
    12001 cgatcccagc gaccaactct tcctgatgca atatttttac tggtaataag gacagtaatc
    12061 tcatgaaaat caaaactatt ttaacaccag tagcctgtgc tctgctgatg agcttttccg
    12121 cccatgccgc taacgctgac aattataaaa acgtgattaa ccgtactggc gcgccgcagt
    12181 acatgaagga ttacgattac gacgatcacc agcgttttaa tccgtttttc gatctcggag
    12241 cctggcatgg tcatctgttg ccagacggcc cgaacactat gggcggcttt ccaggcgttg
    12301 cgttgttgac ggaagagtac atcaacttta tggccagcaa tttcgaccgc ctgaccgtct
    12361 ggcaggacgg caagaaagtc gatttcacgc tggaggcata cagtattccc ggcgcgctgg
    12421 tgcaaaagtt gatatcgaaa gatgtacagg tcgaaatgat tctgcgcttc gccacgccgc
    12481 gcacatcact actggaaacc aaaatcacca gcgataaacc gctggatctg gtgtgggatg
    12541 gcgaactgct ggaaaaactg gaagcgaaag aagggaaacc gctttccgat aaaaccattg
    12601 ctggcgagta ccctgactat cagcgcaaaa tcagcgccac ccgcgatggc ctgaaagtta
    12661 cctttggcaa agtgcgcgcc acctgggatc tgctgacctc cggtgaatca gaatatcagg
    12721 tgcataaatc attaccgatg cagactgaaa tcaacggcaa tcgctttacc agtaaggcgc
    12781 atatcaacgg atcgacgacg ctctatacca cctattccca tctgctgacc gctcaggaag
    12841 ttagcaaaga gcaaatgcag atccgcgata ttctggcgcg tccggcgttt tatctcaccg
    12901 cctcgcagca acgttgggaa gaatacctga agaaaggatt aaccaatccg gatgcgacgc
    12961 cggaacagac gcgcgtcgcg gtgaaagcca tcgaaacgct caacggtaac tggcgatcgc
    13021 cgggcggtgc ggtgaaatat aacaccgtca cgccgtcggt gaccgggcgc tggttctccg
    13081 gcaaccagac ctggccgtgg gatacctgga agcaggcgtt tgcgatggcg catttcaatc
    13141 cggacatcgc caaagagaat atccgcgcgg tcttctcctg gcagatccag ccaggcgacc
    13201 gcgtgcgtcc gcaggatgtg ggctttgtcc ccgacctgat agcgtggaac cttagccccg
    13261 agcgtggcgg cgatggcggc aactggaacg aacgtaatac caaacccagc cttgccgcct
    13321 ggtcagtaat ggaagtgtat aacgtcaccc aggataaggc ctggttggca gagatgtacc
    13381 cgaagctggt ggcctatcac gactggtggt tacgtaaccg cgatcacaac ggcaacggcg
    13441 tgccggaata tggcgcgacc cgcgacaaag cccacaacac cgagagcggc gagatgctgt
    13501 ttacggtgaa aaaaggcgac aaagaggaga cgcagtctgg gctgaacaac tacgcccgtg
    13561 tggtggagaa aggccagtac gacagtctgg aaattccggc acaggttgct gcgtcgtggg
    13621 aatcaggtcg tgatgacgcc gccgttttcg ggtttatcga caaagaacag ctggataaat
    13681 atgtcgctaa cggcggcaaa cgtagcgact ggacggtgaa attcgccgaa aaccgcagtc
    13741 aggacggaac gttgctgggc tactcgctat tgcaggagtc ggtggatcag gccagctata
    13801 tgtacagcga taaccattat ctggcggaga tggcgacgat tctcggtaag ccggaagagg
    13861 ccaaacgcta tcgtcagtta gcacaacagc tcgcggacta catcaacact tgtatgttcg
    13921 acccgactac acagttctac tatgacgtgc gtattgaaga taaaccgctg gcgaacggct
    13981 gcgcgggcaa accgattatt gagcgcggta aagggccgga aagctggtcg ccgctgttta
    14041 acggtgaggc aacgcaggcc aatgccgacg cggtggtgaa ggtgatgctg gatccgaaag
    14101 aattcaacac ctttgtcccg ctgggaacgg cggcgttaac caatccggct tttggcgcgg
    14161 atatctactg gcgcgggcgc gtatgggtgg atcagttctg gtttggtctg aaagggatgg
    14221 agcgttacgg ttatcgtgat gatgccctga agctggcgga tacgttcttc cggcacgcca
    14281 aaggattaac cgccgatggc ccaattcagg agaattacaa cccgctcact ggctcgcaac
    14341 aaggcgcacc aaatttctcc tggagtgccg cgcatttgta tatgttgtat aacgattttt
    14401 tccgtaagca gtaatttgca acggctggcg gatgcggcgt gaacgcctta tccgccctac
    14461 atgtgcaatc ccccaggccg gataaaacgc ggcaagcgtc gcatccggcg ttaccaccgg
    14521 gcgtattctt tttgaatccc atcacaaacc ccgcactccc cttttccctt ttctccggcg
    14581 acggctaaat tagaactcat ccgaccacat aacaattatt ttacatactg gacaatttta
    14641 tgagctaccc gtcgctgttc gccccgctgg atttaggttt taccacgtta aaaaaccgcg
    14701 tgttgatggg ctcaatgcac accgggctgg aggagtaccc ggacggcgct gaacggctgg
    14761 cagcgtttta tgccgaacgc gcccgtcacg gcgtggcgtt gattgtcagc ggcggtatcg
    14821 caccagattt aacaggcgtt ggcatggaag gcggtgcaat gctcaatgac gccaaccaga
    14881 tcccacacca tcgcaccatt accgaagccg tacatcagga aggcggcaaa atcgccctgc
    14941 aaattttgca taccgggcgc tacagctacc aaccgcatct ggtcgctccc tccgcattgc
    15001 aggcccccat caaccgtttc gttcctcacg aactcaccca tgaagagatc ctgcaactga
    15061 tcgacgattt cgcccactgc gcgcaattgg cacgggaggc aggatacgac ggtgtagagg
    15121 tgatggggtc cgaagggtat ttgatcaacg aatttctgac gctgcgcacc aatcagcgta
    15181 gtgaccagtg gggcggcgat taccgcaacc ggatgcggtt tgccgtagaa gtagtgcgtg
    15241 cggtgcgcga acgtgtcggc aacgacttca ttattatcta ccgtttgtcg atgctcgacc
    15301 tggtcgaagg cggcggtact tttgccgaaa tggtagagct ggcgcaggcc attgaagcgg
    15361 cgggcgcgac cattatcaac accggcattg gctggcatga agcgcggatt ccgaccatcg
    15421 ccacgcccgt gccgcgcggc gcctttagct gggtcacgcg caaactgaaa gggcacgttt
    15481 cgctgccact ggtgaccacc aaccggatta acgatccgca ggttgccgac gacattctct
    15541 cgcgcggtga tgcggacatg gtatcgatgg cgcgaccgtt tctcgctgat gcggagctgc
    15601 tatcaaaagc gcaatcggga cgagccgatg agatcaacac ttgtattggc tgcaaccagg
    15661 cctgtctcga tcagatcttc gttggcaaag tcacctcgtg cctggtgaat cctcgcgcct
    15721 gccacgaaac caaaatgcca atccttcctg ccgtgcagaa aaaaaatctg gcggtggtcg
    15781 gtgcgggacc tgctgggctg gcgtttgcca ttaacgcggc ggcgcgtggg catcaggtaa
    15841 cattgtttga tgcgcacagc gagatcggcg ggcagtttaa tatcgccaaa cagatccccg
    15901 gcaaagagga gttttacgaa acgctgcgct attaccgccg gatgatcgaa gtgacgggtg
    15961 taacgctaaa actcaatcac accgtgacgg cggatcagtt acaggcgttc gatgaaacga
    16021 tcctcgccag tgggatcgtg ccgcgcgttc cgcctatcga cgggatcgat catccgaagg
    16081 tactgagtta tctcgatgta ctgcgcgaca aagcgccggt gggcaacaaa gttgccatca
    16141 tcggttgtgg cggaattggt tttgatacgg cgatgtattt aagtcagccg ggcgaatcca
    16201 ccagccagaa tatcgccggg ttctgtaatg agtggggaat cgacagtagc ctgcaacagg
    16261 ccggaggttt gagtccgcaa ggcatgcaaa tcccccgtag cccacggcag attgtgatgc
    16321 tccagcgcaa agccagcaaa ccaggacaag ggttaggcaa aaccaccggc tggatccatc
    16381 gcaccaccct gctctcacgc ggcgtgaaaa tgatcccagg cgtaagttac cagaagatcg
    16441 acgatgacgg gctgcatgta gtgatcaacg gcgaaacgca ggtactagca gtggacaatg
    16501 tggtgatctg cgcggggcaa gagccaaacc gcgcattggc gcaaccgctg attgatagtg
    16561 ggaaaactgt gcatttaatt ggcggctgcg atgtagctat ggagctggac gcacggcggg
    16621 caattgccca gggaacacgg ctggcgctgg agatttaaat cgttgtttga agcgccggat
    16681 gcgacgcatc cggcacgtcg cgttaatcaa taaacaaggc gggagaaata ccgaatcgcg
    16741 ttgccagttt tttagcgtgt tccagcgtta atttcctttt cccgctcaaa acgcgtgaca
    16801 ccatcgattt actgcctatt tccggaagat cggaaagggt taaaccatat tgatccataa
    16861 gggtacgaat cacggctata ccgccaggca tggcttgagc catggcatta aattccacaa
    16921 attcgggcgc tgattcttcc cacgcggtta ttttggcaca caccagatcc agtaaggggt
    16981 tttcaggatc gttgagcagc agatgatcta ccagttccag cgcctgggtg tattgttcct
    17041 cgttctgaat acccgccaga aaaggtgcca cagcagttag cttttctcct gcttgcaaga
    17101 tgtcggcaat cgcaatcatt ttttcccctt agtacgatga acggcggtaa agaaatcgta
    17161 ttctttatgc gtcataactt cacgtatgta gcacttttgc gattcaaaaa agaccattgc
    17221 tacaacacgt aattcattgc ccccgacatt gaaaacataa tgcttatcca gatatttgaa
    17281 gttatccaga gaagggaata ctgcttttaa tgactcaggt tttttgaaat atcccttagc
    17341 aatcgtgttc cccagagcca ccaactccgt tttatgttgc gggtattttt ccgcagcatc
    17401 tttcaatgct ttttgagtta tcaggtgcat tcttcatcac gtccgttgac aaattggcaa
    17461 tatgataaca tccgttgaca gattggcaac gaatgaattt atctgtgtga cggaagaata
    17521 aacgtagaaa aacgctaaac aagagaatgt agaggatggt gacaggaatt ttttgtttta
    17581 tggaagcgcg attccagaac tgggcagaca ttgccggatg cgacgcttgc cgcgtcttat
    17641 ccgacctggg ggtaatgtgc tgaacgtatg ccggataagg cgtttacgcc gcatccggca
    17701 ggtaaccctc aatcttaccg acgacgcccc agcttcactg ctttcagcac cacaaattta
    17761 ttattcgtgg cgatggtggt gcagttgccg aaaattttct tcagtttatg gaagtaatcc
    17821 aagtgacggt tggcaacgat atacagctcg ccgttgattt tcaggcagcg gcgggcgtgg
    17881 tggaacatct cccaggcgac gttatcggtc agcgcatgtt gctgatgaaa cggcgggttg
    17941 cagagcacag cattaaaacg gaaaggctcc acgccggaaa gcgcgttgtt gatcataaac
    18001 tcgcagcgat ccagtgcctc gggcatgttg gtttcaacgt tcaaacggct ggaagcaacc
    18061 gccatcggtg attcatcgac aaacaccact ttcgcctgcg ggtttttatc gagcaatgtc
    18121 aggccaataa cgccattacc gcaaccgaga tcgacaatct caccttcgag attctctggc
    18181 agatgttgca taaagaagcg cgcgccaata tccagcccgg tgcgggagaa gacattcgca
    18241 tggttgtgga tagtccagtc agtaccttcc agcttccagc taacggtctg cggcgcatcg
    18301 gccaacggcg gttcgttgaa ggtgcagtta atcaggcgcg ctttcttcca tgccagcgtg
    18361 gtggtggtcg ggccgagcac tttttcgaac aattccagcg tggaagtgtg aatgtcacgg
    18421 gctttggcac cagcaataat gcgcgtatct ggcgtgacca ctttgcgcag cgcgcgcagt
    18481 tgttgttcca gcaatgccag tgttttcggc actttgatca gcaccacacc cggctgttgc
    18541 gggtagtcgg cggtgctgtc gagaaacttc acgctcgact catcaatccc gttgaggcgt
    18601 aaattctcgc gcgtggccag ttcactgatg tatgagtcgc caatgctgta cggcttatgt
    18661 tccgccagca cacagcttaa cgcaccaaaa gcatcattca ggatcaacac cgggccgcgg
    18721 atttctgtgt cgtccaactg ttgcagcaaa tactcatccg ccgcttccca cgcctgtagc
    18781 gggttaacgt catccgtcgc cggaaaacgt tgtagtgtca gtgaacggaa accgttgtct
    18841 aagtggctca tcggccctcc tgaatgataa aatttcgacg ttgcccctga aaagggtgcg
    18901 tgagtatacc ctttttctct ttttcgtggg cggttatgag caatcttact tatcttcagg
    18961 gttatcccga gcagctactt tcccaggtgc gaacgctgat taacgaacag cgtctgggcg
    19021 atgtgctggc aaaacgttat ccgggaacac acgactacgc caccgataaa gccctctggc
    19081 aatatactca ggatcttaaa aatcagtttc tgcgcaatgc cccgccgatc aataaagtga
    19141 tgtatgacaa taagatccac gtgctgaaaa acgcgctcgg gttacatact gccgtttcgc
    19201 gtgtgcaggg cggcaagctg aaagcaaaag cagagatccg cgtcgctacc gtgtttcgca
    19261 acgcgccgga accgtttttg cgcatgatcg tggtgcacga gctggcgcac ctgaaggaga
    19321 aagagcataa caaagcgttt taccagttgt gctgtcatat ggaaccgcag taccaccagc
    19381 ttgagttcga cacccggctg tggctaacgc agttgtcgct tgggcaggac aaaatctgaa
    19441 aatgatgtca ggcgttattt tttcctcctt tagcgcctaa aatcgatctc ctccctttcg
    19501 ttaaggataa cgaccatgct gcgcgcattt gcccgccttc ttcttcgtat ttgtttttct
    19561 cgccggactc ttaaaattgg ctgtttattg ttgcttatag caggagcgac catttttatt
    19621 gccgatcgcg ttatggtcaa cgccagcaag cagctgacct ggagtgatgt caacgccgtt
    19681 ccggcgcgga acgtgggttt attgctgggg gcgaggccgg gtaatcgcta ctttacccga
    19741 cgtattgata gcgccgcaga gttgtaccac gccggaaaag tgaaatggct attggtgagc
    19801 ggcgataatg gacgtaaaaa ttacgatgaa gcgtcaggaa tgcagcaagc cttgatcgct
    19861 aaaggcgtac cggcaaaagc catcttctgc gactatgccg gattctcaac gcttgattcg
    19921 gtggtgcgtg ccaacaaggt gtttagtgaa aaccatatca ccattatctc gcaagaattt
    19981 cataaccaac gcgccatctg gctggcaaaa cagtatggta tcgatgctat cggttttaac
    20041 gcgccagacc tgaatatgaa gcacggttta tatactcaac tgcgggaaaa acttgccaga
    20101 gtcagcgcgg tgattgatgc gaaaatcctc catcgccagc cgaaatatct cgggccgttg
    20161 gttatgattg ggccgtttag tgagcatggc tgtccggcaa aagaataatg cgtatctgcg
    20221 cacgtcgaag ataaaaaagg cgtgctacag tgacgacaga atccctttat ggagtatcca
    20281 cgcgttatga tacgtttcgc tgtgattggt acgaactgga tcactcgcca gttcgtcgag
    20341 gccgcccatg agagcggtaa atacaagtta accgccgtct attcccgcag ccttgaacag
    20401 gcccagcact tcgccaatga tttttctgtc gagcatctgt ttacgtcgct ggaagcgatg
    20461 gcagaaagcg atgccattga cgcggtgtat attgccagcc caaattccct gcacttttcc
    20521 cagacacaac ttttccttag tcacaaaatt catgtgattt gcgagaagcc actggcgtcg
    20581 aatcaggcgg aagtggatgc cgccattgcc tgtgcgcggg aaaatcaggt ggtgctgttt
    20641 gaggcattta aaacggccta cctgccgaac tttcatctgt tacgccaggc gctgccgaaa
    20701 gtcggcaaac tgcgtaaagt ctttttcaac tattgccagt attcctcgcg gtatcagcgt
    20761 tacctggacg gagagaatcc caacaccttc aatccggcat tctccaacgg ttcgattatg
    20821 gatatcggct tttactgcct ggcgtcggcg gtggcattat tcggtgaacc gaaaagcgtg
    20881 caggcaaccg ccagtttgct ggcaagcggt gtggatgccc acggcgtggt ggtgatggat
    20941 tacggtgatt tcagcgttac gttgcagcac tcaaaagtga gcgattctgt cctggcaagc
    21001 gagattcagg gcgaagcggg atcgctggtg attgaaaaac tctctgaatg ccagaaagtc
    21061 tgctttgtac cgcgtggcag ccagatgcag gatctcaccc agccacagca tattaatacc
    21121 atgctctacg aagcagagct gttcgctacc ctggtggatg agcatctggt gaatcatcca
    21181 gggctggcgg tcagtcgtat caccgccaaa ctgctgactg agatccgccg ccagactggg
    21241 gtgatttttc cggcagataa cgtaaaacta taattgccaa agtaaaacag tgtaaaaggt
    21301 atgtaacaga ccattgactg actgaatggt ctgtcatact ttgttacctg caaaggggag
    21361 taacttcatt gtcggtcgat cgtcattacg gtgtgtgaaa aaccacatcc ggtcaccggg
    21421 caacccgaaa ggaatacgca gacgtattcc ttttttgttg taagtgagac cttgccggaa
    21481 ggcgaggtct atgcataaaa agcagcggct gacgtcttcc gacgttggcc gtttttttat
    21541 gtgtaaggaa cttctatgaa tactgtcggc acgccgttgc tatggggcgg attcgctgtt
    21601 gttgtcacca ttatgctggc tatcgacctg ttgttgcagg ggcgtcgtgg ggcacatgcc
    21661 atgaccatga aacaggctgc ggcctggtcg ctggtctggg tgacgctgtc gttactgttt
    21721 aacgccgctt tctggtggta tctggtgcaa accgaaggtc gcgccgttgc cgatccacag
    21781 gcactggcct ttctcacggg ttatctgatt gagaaatcgc tggcggtcga taacgtcttt
    21841 gtctggctga tgttgttcag ctatttctct gttccggcgg cattacaacg ccgcgtgctg
    21901 gtgtatggcg tactcggggc gattgttctg cgtaccatca tgatcttcac tggtagctgg
    21961 ctgatttcac agttcgactg gatcttgtat atcttcggtg ccttcctgct gtttaccggc
    22021 gtgaagatgg cgctcgccca tgaagatgaa tcaggcattg gcgacaagcc gctggtgcgc
    22081 tggctgcgcg gtcatttgcg catgaccgac accatcgaca acgagcattt ctttgtgcgt
    22141 aagaatggtt tgttgtatgc cacgccgctg atgctggtgc tgattctggt ggagttgagc
    22201 gacgtgattt tcgccgtgga tagtattccg gctatcttcg ccgtgaccac cgatccgttt
    22261 attgtgctga cctcaaacct gtttgcgatc ctcggcctgc gtgcgatgta tttcctgctg
    22321 gcaggtgtgg cagagcgttt ctcgatgctc aaatatggtc tggcggtgat tctggtgttt
    22381 atcggtatca agatgctgat tgtcgacttc taccatattc cgatcgccgt ctcgctgggc
    22441 gtggtgtttg gcattctggt gatgacgttt attatcaacg cctgggtgaa ttatcggcat
    22501 gataagcagc gggttggatg atttttaatc tgcctaagcc gtgtaccctg tcatccacat
    22561 gagcaccgtt ttctccctct ccccaaaagg ggcgagggga ctgaccgagt acttttttga
    22621 cgttgtcatc agtctggaag ccgcacgttg ggctttattt tcatgtcaaa caaatgtaac
    22681 cattaagttg cgaaatatga cttctcctta aaatccagca tatttcgctt cccgaagctg
    22741 taactttcct tatactcgac cttgcaaaca ctttgttaca tcctgaaaga tgcgtcgaca
    22801 gaacgcacca gggatgtgcg acaacacaat gaaaggatcg aaaaatgact acgcaacatt
    22861 caccggggct attccggcgt ctggctcatg gcagcctggt aaaacaaatc ctggtcggcc
    22921 ttatactggg gattcttctg gcatggatct caaaacccgc ggcggaagct gttggtctgt
    22981 taggtacttt gttcgtcggc gcactgaaag ccgttgcccc catcctggtg ttgatgctgg
    23041 taatggcatc tattgctaac caccagcacg ggcagaaaac caatatccgc cctattttgt
    23101 tcctctatct gctgggcacc ttctctgctg ctctggccgc agtaatcttc agttttgcct
    23161 tcccttctac cctgcacttg tccagtagcg cgggtgatat ttcgccgccg tcaggcattg
    23221 tagaagtgat gcgcgggctg gttatgagca tggtttccaa ccccattgat gcgctgctga
    23281 aaggtaacta catcgggatc ctggtgtggg caattggcct cggtttcgca ctgcgtcacg
    23341 gtaacgaaac caccaaaaac ctggtcaacg atatgtcgaa tgccgtcacc tttatggtga
    23401 aactggtgat tcgcttcgca ccgatcggta tttttggtct ggtttcttct accctggcaa
    23461 ccaccggttt ctccacgctg tggggctacg cgcaactgct ggtcgtgttg gttggctgta
    23521 tgttactggt ggcgctggtg gttaacccct tgctggtgtg gtggaaaatt cgtcgtaacc
    23581 cgttcccgct ggtgctgctg tgcctgcgcg aaagcggcgt gtatgccttc ttcacccgca
    23641 gctctgcggc gaacattccg gtgaatatgg cgctgtgtga aaagctgaat ctggatcgcg
    23701 atacctattc cgtttctatt ccgctgggag ccaccatcaa tatggcgggc gcagcaatca
    23761 ccattaccgt gttgacgctg gctgcagtta atacgctggg tattccggtt gatctgccca
    23821 cagcgctgct gttgagcgta gtggcttctc tgtgtgcctg tggcgcatcc ggcgtggcgg
    23881 ggggatctct gttgctgatc ccactggcct gtaatatgtt cggtatttcg aacgatatcg
    23941 ccatgcaggt ggttgctgtc ggctttatta tcggcgtatt gcaggactcc tgtgaaaccg
    24001 cgctgaactc gtcaactgac gtgctgttca ctgcggcagc ttgccaggcg gaagacgatc
    24061 gtctggcaaa tagcgccctg cgtaactaat acttagcccc tttcgtctac ggcggaaggg
    24121 gttttctcca ctttaaacgg atcaattccc cttctctgca tacgccagaa acgaatgata
    24181 ttcaggccat tcatcagcag aaaactgcct tcaatcatcg tgccgcctat cgaccccgcc
    24241 cagaagttgt gaatcaccca gcaacacgtt gaaaaccaca ttacgcagcg catggtcagc
    24301 cctttacagc ggaacagcgc ccaggtactg acaatcgtgc cgattaccgg caatagttca
    24361 acaggatgat ggaacttcgc gaggccaatt ccgccagtca gcacaataaa aatcgccatt
    24421 acccataagc tgcgcgtgcg taaggtaatc aatgtacgaa tggcattaag gatggcactg
    24481 gcaccggcag gataggtgcc cagaagaaaa aaatgtacgc caataacggc gctatagacc
    24541 gaaagttgct ttttgaagcg acgttcgtca cgattgaaaa atgttgtgat accaatcaga
    24601 aaggcgatga cacccacgcc ctgggccagc caatacgcgg tcatgataaa tccttagcag
    24661 gtatggaaaa gcaaacggcg cttcacatta tgaaacgccg ttttttatta acaactcatt
    24721 tcgactttat agcgttacgc cgcttttaaa gatcgccagt tcgcggaagt cgttacgctc
    24781 gttacaggtt tgcttaccgt tggcaaactc aacgatggtg tcgataaatt cttccagcaa
    24841 ctgcggcatc gctttaccgt ggatcagctg acccgcgtca aagtcgatcc agtgtttttt
    24901 cttcgccgcc agttcactgt tggtggcgat tttcaccgtc ggcacaaatc caccatacgg
    24961 cgtgccacga ccagtactga acagcaccat atggcagccc gcacccgcca gggcgctggt
    25021 cgctacggca tcgttacccg gcgcacttaa caagttcagc cccggcgttt tcagacgctc
    25081 gccgtaacgc agcacgtcaa ccacgacact ggaacccgct ttctgggtac agccaagcga
    25141 tttatcttcc agcgtggtga taccgcctgc tttgttcccc ggtgatgggt tttcgtagat
    25201 cggctggtca tgggcaataa agtactgctt gaagtcattg accatggtga ccagtttttc
    25261 aaacgttgct tcgtcgcggc aatggtccat cagcaactgc tctgcgccaa acatctccgg
    25321 cacttcggtc agtacggtag taccgccgtt agcaatcacg tagtcagaga aacgccccag
    25381 catcgggtta gcagtaatac cagaaagacc gtcagaacca ccgcactcca gaccaaactt
    25441 cagttcgctg agtttgcccg gctcgcgttt atcgttgcgc atcacgctat acagctgatg
    25501 caaatgcgcg atcccggcct cgatttcatc atcctgctgt tggcagatca tgaaatgaac
    25561 gcgttcagga tcgatatcgc ccaacgtttc acggaatgcg gcaacctggt tattttcgca
    25621 acccagacca atcaccagca ctgcgcccgc gttcgggtgg cgcaccatgt tttgcagcat
    25681 ggtgcgggta ttgatgtgat cgtcgcccag ttgtgagcag ccgtaggtgt ggctgaagag
    25741 gaacacgccg tcggtacctt cagcgttgtt ggtctctttc aggaaacgat tttggatctg
    25801 ccgcgcgatg ccgttgacac agcccacggt tggcaggatc cacagctcat tacgcacccc
    25861 gacatcaccg ttagcgcgac gatagatctg cacttcacga tctgccgctt gcgcaggcag
    25921 atcctgaaaa tcaggttgat agcgatactg atccagatcg ctcagattcg tgcgcgtatt
    25981 gtgggcgtga acgtgttccc ccgccgcaat atccgccaat gcataaccaa tcggcaggcc
    26041 atatttaatg acgttggccc cttttgcgat atccgttaac gcaaatttat gtccacgagc
    26101 aacatcctgg cgcagcgtaa cagtctggtt atcgacactg acttctgtgc cttcagccaa
    26161 atccgctaaa gcaaccgcga cgttatccag cgcatggatc ttgatgtatt gcatatcaac
    26221 cccagacctt agttcagttc aatggcgaag tagtcacgcg cattgttaaa gcaaatattt
    26281 ttcaccatct cgcccagcag gttgatgtcc gccggtgctt cgcctgcttc cacccagcga
    26341 ccgatcatct ggcacagaat gcggcggaag tattcgtgac gggtgtatga caggaagcta
    26401 cggctgtcag tcagcatgcc gacaaagcgg ctcagcagac cgagctgcgc cagttgggtc
    26461 atctgacgtt ccataccgtc tttctggtcg ttaaaccacc agccggaacc gaactgcatt
    26521 ttgcccggca taccttcgcc ctggaagtta ccgatcatgg tgcccagcac ttcgttatcg
    26581 cgcgggttca ggcagtagag aatggttttc ggcagcaggt tttcttcgtt ctgcttgctc
    26641 agcagcttag atagctcttc cgccatcgga cggtcgttga tggagtcaaa gccgacatcc
    26701 ggccccagca gtttgaactg acgcaggttg ttattacgca gtgcgccaat gtggtactgc
    26761 tgtacccatc cgcgacgtgc atattcggca ccaaggaaca ccagcaccgc agttttgaac
    26821 tgtgctactt cgtgctcgct cagggtttcg cctgccagac ggcgcgcgag gatgctgtcc
    26881 agttccgctt cgttcgcttc agcaaacatc accacatcca gcgcgtggtc agacacttta
    26941 cagccgtgag cggcgaagtg atccagacgt ttagtcaggg cagtttgcag gtcagcaaag
    27001 cggcgaatgt cggtatcaga aacttcgccc agcttcgcca tgtagtcgtt aaaggtcgcc
    27061 tgttcaatgt taaaggcttt gtccggacgc cagctcggca gcactttaat ggtgaaagag
    27121 ccgtctttgg cgatctctgc gtgatgctcc agagaatcga tcgggtcatc ggtggtgccg
    27181 accattttca cgttcatctg ctgcatgatg ccgcgcgcgg agaagttatc ctgcgccagc
    27241 agttcgttac attcgttcca gatttcatcg gcagttgacg gagaaagcaa tttgccagtg
    27301 ataccaaacg gacgacgcag ttcgaggtgc gtccagtggt ataacgggtt gccgatagtg
    27361 tgcggaacag tcgccgccca ggcgtcaaat ttttcacggt cagacgcatc accggtgcac
    27421 agacgctcgg ccacaccgtt ggtacgcata gcgcgccatt tgtagtgatc gcccttcaac
    27481 cagatgtcat acaggttttt aaaacgatag tcttccgcaa tctgctgcgg cggcaaatgg
    27541 caatggtaat cgaaaatcgg ctggtctttt gcgtagtcgt gatacagacg gcgggcaaat
    27601 tcagtatcta acaggaaatc ttcagtcata aacggagtca ttttcgtctt cctctcaacg
    27661 agtgagctag cttgcttatg gtgcgatgct gacaaagtta tcacaccaat ttccagagtc
    27721 cgaagatatt ttcgtgagtt agatcaataa acgtagttaa aaaaattact ctcaaagtgg
    27781 taaatctcgc tgcaggccgc gccagtactg gcctcgtcgt cgtcaggtaa tgtccctaca
    27841 aatattcgca catttgtgat ggctctcacc ttttaaagtt gtatgacaag ttatctttct
    27901 gccgtcgcaa atcataagtc gacggaatgc aaattgccga ttcattcatt tgttagatga
    27961 atcgggttaa ccggtacgga agccgaatta gcacgaaact ttcatggcaa cgttcggggc
    28021 gtgccggttt tttttcggtt acccggtcgt aactaacatc ttcagcctct ggcgggatga
    28081 tggccgcgct tcctgcggat ataacaaaac gatgaggttt tacatgcgta aaattaaagg
    28141 gttacgttgg tatatgatcg cactggtgac gctcggcacc gtgcttggtt acctgacgcg
    28201 taacactgtg gcggcagctg cgccaactct gatggaagag ttaaacatct ccacccaaca
    28261 gtattcctat atcatcgcag cgtattctgc tgcttatacg gtcatgcaac cggtagcggg
    28321 ttatgtactg gatgtgctgg gtacgaaaat cggttatgca atgtttgctg tactgtgggc
    28381 cgtgttctgt ggtgcaaccg cgctggcagg tagttggggt ggcctggcgg ttgctcgtgg
    28441 tgcggtcggt gccgcggaag ccgcgatgat tccggcgggt ctgaaagcca gctccgaatg
    28501 gttcccggca aaagagcgtt ccatcgcggt aggttacttt aacgtaggtt cttcgattgg
    28561 tgcgatgatt gcgccgccgc tggtggtatg ggcaatcgtg atgcacagct ggcagatggc
    28621 atttatcatc tccggtgcgt tgagctttat ctgggcgatg gcatggctga ttttctacaa
    28681 gcatccgcgc gaccagaaac atctgaccga tgaagaacgc gactacatta ttaatggtca
    28741 ggaagcccag caccaggttg acacagcgaa gaaaatgtcc gttggtcaga tcctgcgtaa
    28801 ccgtcagttc tggggtatcg cgctgccgcg cttcctggca gaaccggcat ggggtacttt
    28861 taacgcgtgg atcccgctgt tcatgtttaa agtttacggc tttaacctga aagagatcgc
    28921 gatgtttgcc tggatgccga tgctgtttgc tgacctcggt tgtatcctcg gtggttacct
    28981 gccaccactg ttccagcgtt ggtttggtgt gaacctgatc gtttcccgta agatggtcgt
    29041 aacgctgggt gcggtgctga tgattggccc gggtatgatc ggtctgttca ccaacccgta
    29101 tgtcgcgatt atgctgctgt gtatcggtgg ttttgctcac caggcactgt ctggcgcact
    29161 gattacgctc tcgtctgacg tgttcggtcg taacgaagtg gcgacggcga acggtttaac
    29221 cgggatgtcc gcatggctgg caagtacgct gtttgcactg gtagtcggtg cactggctga
    29281 caccatcggc ttcagcccgc tgttcgcagt tctggcagtg ttcgacctgt taggtgcgct
    29341 ggttatctgg accgtgttgc agaacaaacc agcgattgag gtggcgcagg aatcacataa
    29401 cgatcccgca ccgcaacatt aaggcttcgt gtaagtttta gtttcccttc aaagccgcct
    29461 tctcaggcgg ctttttcatc actgcgagta gagctaaact cgcaaaagtg gtataacaaa
    29521 tatagtctgc cgtatcatgc ctggagcgca tatggaaatc actgaaccac gccgtttgta
    29581 tcaacaactt gccgctgatc tgaaagagcg catcgaacag ggcgtctatc tggtgggtga
    29641 taaactgcct gccgaacgct ttattgccga tgaaaaaaac gtcagccgca ccgtggttcg
    29701 tgaagccatc atcatgctgg aagttgaagg ctatgtcgaa gtgcgtaaag gttcaggtat
    29761 tcatgtggtt tccaaccagc cgcgccatca gcaggcggct gacaataata tggaattcgc
    29821 caattacggt ccgtttgagt tgctccaggc tcgccagctc atcgaaagta atattgccga
    29881 gttcgcggca actcaggtca caaaacagga catcatgaaa ttgatggcca ttcaggagca
    29941 ggcacgcggc gaacaatgct tccgtgattc cgagtgggat ttgcagttcc atattcaggt
    30001 agccctggcg acgcagaact ccgccctggc ggctatcgtt gaaaaaatgt ggacccagcg
    30061 tagtcataac ccgtactgga aaaaactgca cgaacacatt gatgcccgta ccgtcgataa
    30121 ctggtgtgat gaccacgatc aaatcctcaa ggcgctgatt cgcaaagatc ctcatgctgc
    30181 taagctggca atgtggcagc acctggaaaa caccaagatc atgttattta acgaaaccag
    30241 cgacgatttc gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tggtgcatct
    30301 cgatacagcc accagcggca gtaaatgaaa ttcctgtccg acaggcgcta cagtgcgcct
    30361 gtttgtctcg tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgcctc
    30421 cctcccctgc attcagcaaa atcagactcc atggacatgt aattttgata acgaacaacg
    30481 ttgacctttg ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc
    30541 acgatttaac agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa
    30601 gacagcaata acattgcggt agtgcatcat ttaaaaccag cgtggcgtta accgattcac
    30661 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
    30721 gaaaccctgg ccaatccgtc gatgattggc atgttgtatt ttgtcttgtt tgtcattttg
    30781 ttccttgaaa acggcttgct tccggcggcc tttttaccgg gcgacagttt actggtactg
    30841 gtcggcgtat tgattgcgaa aggcgcgatg ggctatccgc aaacgattct gctgctgacc
    30901 gttgccgcca gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
    30961 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
    31021 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc gtttgtcaga
    31081 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc
    31141 aactggatga gtggtctgct gtgggtgttg atcctgacaa ctctgggtta catgctcggc
    31201 aaaacaccgg tatttttaaa gttcgaggac cagctgatgt catgcctgat gctgctcccg
    31261 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
    31321 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcac ttcgccagct agcctggtcc
    31381 ggcgctgttt tacttctggt cagcacacta ttgctggcct ggtccgcggt tcgccagcaa
    31441 gagtctacgc tggcgattcg tgccgttcat caagggacaa cgatgccaga cggtttttca
    31501 atctggcatc accttgacgc tcacggcatt cctttcaaaa gtatcacccc caaaaacgac
    31561 actctgttaa ttacctttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
    31621 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
    31681 tggctgaccc ggttgcggga taactctcat cgcttcggat aacttcctgt tatccgaaac
    31741 agatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
    31801 acgaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
    31861 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
    31921 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
    31981 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
    32041 gggccaactg ttcagatagc caactgcgtg ccgatcatca gaagaaaatc gcaaagcaga
    32101 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgcag
    32161 ataagattgc caaacgcgaa cggaaactgg cagaagcgca ggaagagctg aaaaagctgg
    32221 aagcgcgcga ctactaactc acaatagtca ctacttactc acctggagaa aactatgtcg
    32281 aaagaacaca ctacggaaca tctgcgtgct gagttgaaat ccctttccga tacgctggaa
    32341 gaggtgctta gctcatctgg cgagaagtca aaagaagagt tgagtaagat tcgtagcaaa
    32401 gcggagcagg cactgaaaca gagccgttat cgcctgggtg aaaccggtga tgccattgcc
    32461 aaacaaaccc gtgtcgcggc ggcgcgtgcc gatgagtatg tgcgtgaaaa tccgtggacg
    32521 ggcgtgggca ttggcgcagc aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa
    32581 ttatggcgga cactcatcac gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc
    32641 gaattgtttc catcatggtt gaaatggtgg agacacgtct gcggctggcg gtggtggagc
    32701 tggaagagga aaaagcgaat ctctttcaac ttttactgat gctgggcctg acgatgcttt
    32761 tcgctgcatt tggtcttatg agcctgatgg tgctaattat ctgggcggtt gatccgcaat
    32821 atcgcctgaa tgcgatgatt gccaccaccg tggtgttgct gctactggca ctgattggcg
    32881 gcatctggac gttacgtaaa tcgcgtaagt ctacgttgct gcgccacacg cgccatgagc
    32941 tggcaaacga tcggcagctg ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa
    33001 cgacgtaagg cgcaactgct tagccagatc cagcaacaac ggctggatct ttccgccact
    33061 cgtcgtgaat ggctggaggc aacaggcgct tacgatcgtc gctggaatat gctgttgagt
    33121 ctgcgctcct gggcgctggt tggcagtagc gtgatggcta tctggacgat tcgccatcct
    33181 aatatgctgg tccgctgggc cagacgcggt tttggtgtat ggagcgcctg gcgtctggtg
    33241 aaaacaaccc tcaagcaaca acagcttcgc ggttaacttc ctctctggcc ggagaaattc
    33301 cggccttatt cctcaaattt tttgaaaata tttgacagtt ttccttgcta acaatcacgc
    33361 ttcgccacgt ttatgattct ctccatcgac agcaacgacg ctaatagcgc gcccttgcac
    33421 aaaaaaacaa tcagcagcct gagcggcgca gtggagagta tgatgaaaaa attagaagat
    33481 gttggtgtac tggtagcgcg tattttaatg ccgattctgt ttattaccgc aggctgggga
    33541 aaaattactg gctacgcggg tacccaacaa tatatggaag caatgggcgt ccccggcttt
    33601 atgctgccgc tggtgattct gcttgagttt ggtggtggtc tggcaatcct gttcggtttc
    33661 ctgacacgta ccacagccct gtttactgcg ggctttacgc tgctgacggc atttttattt
    33721 cacagcaatt ttgcagaagg catgaactcg ctgatgttta tgaaaaacct gactatttct
    33781 ggcggattcc tgctgctggc aattaccggt ccaggcgcat tcagcatcga ccgcctgctg
    33841 aataaaaagt ggtaagcacg cgctatactt aacgataaaa aacgaggggg aagctcctcg
    33901 tttttgctat tggaggatag aaaaatgggt caactgattg acggcgtctg gcatgacacc
    33961 tggtacgata ccaaatccac cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac
    34021 tggctcactg ccgatggcgc gccaggcccc actggcaaag gcggttttgc cgcagagaaa
    34081 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cacaccgcac gctgatcatg
    34141 cgtaagctca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
    34201 gaaaacggct ggacctttga tgacagtttt ccgggagcaa gcggcgacac gctctatcaa
    34261 catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
    34321 gttcccgtgc tgtgggacaa aaaaaaccac accatcgtca gcaacgaatc agcagaaatc
    34381 atacgcatgt tcaataccgc gtttgatgcg ctgggtgcga aagctggaga ttactaccca
    34441 ccagccctgc aaccgaaaat tgacgaactt aacggctgga tttacgacac cattaacaat
    34501 ggcgtgtata aagccggttt tgccaccagc cagcaagcct acgacgaggc ggtggcgaaa
    34561 gtgtttgaat cgctggcgcg actggaacag attttaggtc agcaccgtta cctgaccggc
    34621 aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtgcgttt tgatccagtg
    34681 tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa tctgtatggc
    34741 ttcctgcgcg atatctacca gatgccggga attgccgaaa cagtcaattt cgatcacatc
    34801 cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat ttcaattggt
    34861 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca
    34921 ccttacgagg tgcctctgat aattagctaa gtacgtgttg ttcatgtcgg atgcggcgta
    34981 aacgccttat caaacctaca aaacatttat attcaatatg ttgcaatgac cgcgcaggcc
    35041 agataagcgt agcgcatctg gcaattttgc gtttgccctt atccttcaca gcgccttatt
    35101 aattcatcgc gataatattt actgccattt cattcacgtc tattcttaat ttgctgcttt
    35161 aaaaacaagt gattgagcaa aattgaggca aaaatggact ggtatctgaa agtactaaaa
    35221 aattatgtcg gtttccgggg tcgtgcgcgg cgcaaagagt actggatgtt tattctggtc
    35281 aacatcatct ttacgttcgt gctggggcta ctggataaaa tgttaggctg gcaacgtgcc
    35341 ggtggcgaag gcatcctgac gacaatctac ggtattctgg tgtttttacc atggtgggca
    35401 gttcagttcc gccgcctgca cgacaccgac cgctcggcgt ggtgggcact gctattctta
    35461 atcccgttta tcggctggct tattatcatc gtctttaact gccaggcggg tacgccaggc
    35521 gaaaaccgct ttggaccaga tccgaaactg gaacaagaat aataattaag caccttgtaa
    35581 aaggtgctta atcgattatt ttccgctaaa aagtttggga atttcccgca gacaccagga
    35641 tttcgcctcc cccatactgt cacgacgcca ggccataata atatcgatct cgctggtcga
    35701 ttccgggctg acgacacgca accgcccttc cgcaatatct ttttcgacca tcggatacgg
    35761 catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt ttatcttcaa tcgtgctcac
    35821 cgttaagcgc ggctgtttgt ccagcaactg tacggtcaac accgggcgct cacgagcggt
    35881 atccgccacc gcaattccac gatatttcac gcgcgtcact tcagataacg gttccggctc
    35941 ctgatgaatc gggtgatccg gcgcggcaac atagacgttc attaaggtat agagtttgcg
    36001 cgagttgatc tccgacgagg aacgaaaatg catatccggc gcgataacga tatccgcccg
    36061 cccctgctcc agccgttccc acgcccccgc cagcacttcg gtgataattg ccagttgggt
    36121 attggctttt gccgccagtt tgtcgattaa cgggaaaaag gcaggtgtcg gtaccagcgc
    36181 ctcggtcaca atggtgagat gcgtttccca accgcgcgcg agggcttccg catcggtagt
    36241 cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc agcaacatcc tcccgacatt
    36301 ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc acatccagct cttcttccag
    36361 tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc agctcatccg ccgccgccgc
    36421 aaaactgccc cggcgatcga tcgcatccat aacccgtagt gcttccagcg ttaatgccct
    36481 ttctttggcc atttcgttct cattcaggaa atttgaacat accgggcaga atatctggct
    36541 aacaatgtag cgtccagccc cttaccataa aaggaagtaa agagaggtca agaattatga
    36601 ttactacccg aactgccagg cagtgtggac aagcagacta cggatggttg caggcccggt
    36661 atactttttc ctttggacac tacttcgacc cgaaattgtt aggctatgcc tctctgcgcg
    36721 tgcttaacca ggaagtgctg gccccaggtg ccgcctttca gccacgaacc tatcccaaag
    36781 tcgatatttt aaatgtgatt ctggatggag aagctgagta tcgcgacagc gaaggcaatc
    36841 atgttcaggc cagcactggt gaggtgttgc tgctctctac ccagccgggt gtcagctata
    36901 gcgaacacaa tctcagcaaa gacaaaccgt taacgcgaat gcagctttgg ctggatgcct
    36961 gcccgcagcg agagaatccg ctgattcaaa agctggcgct taatatgagc aagcagcaat
    37021 taatcgcctc gccagaaggt gggatgggaa gcctgcaatt gcgccagcaa gtgtggctgc
    37081 accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt
    37141 atttgcaatc gattcacggg aaatttcatg cgcttacgca tcatgaagag aaagcggcgc
    37201 taacctgcgg tgatggggcg tttattcgtg acgaggctaa cattacgctg gttgccgatt
    37261 ccccactgcg cgctttgctg atagatttgc ctgtctagtt gtttttacag ggagatgatg
    37321 atgagtaaaa aatcggccaa aaagcgccag ccagtgaagc ccgtggtggc gaaagaacct
    37381 gctcgtaccg ccaaaaattt tggctatgaa gagatgttga gcgaactgga agccatcgtc
    37441 gcggatgctg aaacgcgttt agccgaggat gaagctaccg cgtaaagaca ggttgccgga
    37501 tgcggcgcaa acgccttatc cggcacaata cccaaaaccg ggcgtttatc tggccttgct
    37561 cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca
    37621 caggttggca atcgactgct caacatcatg cgccacaatc ccttcattgc cggtcacggc
    37681 ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt
    37741 catcgcgcag ctgttcgatg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    37801 gctgatcgcc atcgagatgg tttcataacg cccatccacc agccatgcca tcccggcagc
    37861 ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta actggttatg
    37921 gatgtaaatc gcgctcaaat gcgaaagcat cagcgcacgc gccagccgtt catcatccgc
    37981 tccgaagtgt tcagctacca ccaccacggg cattgttgcg gtgatcccct ggttacccga
    38041 gccggagtta ctcattgccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    38101 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgcg cgcactgttt
    38161 ttccagcgtc gcaccaatat gcagccccca gttaccgctc aaaccttcct gcgataacgc
    38221 acaatttaac ttcgcagaat cgagaataaa gcggatcgcc gcaaacggga cttcattgac
    38281 gaacttcagg atctcagcca gcgtcgtcct ggaaagcacc gtcagcggcg actcttgctc
    38341 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    38401 aatgttggta tgcccgccga cgatggtgac acacgcccat ttctcaccgt tccagacttt
    38461 ggcgcgtgag aagaggattt catcgcaagg ttcctggatc ttaactgaga ctttccccgc
    38521 cgccagcagt gctttggcat cggaaattgc ctgcgcagtt gcgtctttca gcacttccag
    38581 cccggcgtta gcatttccac ctaacgctcc cagcgccgcc gcaattggca gccccaccat
    38641 tcctgtaccg ggaacggtga cacccagacc gttcttcatc agatttggcg aaacccaggc
    38701 ttctacacgt tcaaccggac cttccagttc agccgcagca accgccgccg ccagcgccag
    38761 tgaaatcggt tcagtacacc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    38821 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    38881 aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga cgataatcag
    38941 gtacaaagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    39001 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    39061 ggcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    39121 aacgagattt tcgttaatct tctcggcagg tatcttgcga cgcaggatgt tcattacgat
    39181 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca ttacagcaaa
    39241 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    39301 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    39361 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    39421 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    39481 ataagagatc accatcgggc ttaacgtctg gataaataga atcgacgtca gggtaaacgg
    39541 cagcgtaata atggcgtttt tcatcagcag ccccagcggc ggtagcgaac cgacgttata
    39601 cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca gcttggtcag
    39661 caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaggaga tcgccaccag
    39721 aatgcaaatc agtaccagac cataaaaggg actgtctgac agtgaagtgg tttactgaat
    39781 ttggccacct gaacagaggt gatatgctca cctcagaaca acacaggtgc cataatgaaa
    39841 aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc aactggtcgt tgaccagaat
    39901 tacaccgtgg cagatgcagc cagcgctatg gatgtcggcc tttccacaat gacgcgatgg
    39961 gtgaaacaat tacgtgatga gcggcaggga aaaacaccaa aagcctcccc tattaccccg
    40021 gaacaaattg aaatccgtga gctcaggaaa aagctacaac gtattgaaat ggaaaatgaa
    40081 atattaaaaa aggctaccgc gctcttgatg tcagactccc tgaacagttc tcgataatcg
    40141 ggaaactcag ggcgcgttat cctgtggcca ctctctgcca tgtgttcggg gtccatcgca
    40201 gcagctacaa atactggaaa aaccgtcctg aaaagccaga cggcagacgg gctgtattac
    40261 gcagccaggt acttgaactg catggcatca gccacggctc
//
LOCUS       DAPQZD010000023        39529 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPQZD010000023 DAPQZD010000000
VERSION     DAPQZD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.023"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(103..804)
                     /locus_tag="RO454_001207"
     CDS             complement(103..804)
                     /locus_tag="RO454_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDY0933072.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSDGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGAMK"
     gene            complement(801..1820)
                     /locus_tag="RO454_001208"
     CDS             complement(801..1820)
                     /locus_tag="RO454_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDY0933073.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(1817..2356)
                     /locus_tag="RO454_001209"
     CDS             complement(1817..2356)
                     /locus_tag="RO454_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HDY0933074.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYLEQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2426..2656)
                     /locus_tag="RO454_001210"
     CDS             complement(2426..2656)
                     /locus_tag="RO454_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933075.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            2761..3450
                     /locus_tag="RO454_001211"
     CDS             2761..3450
                     /locus_tag="RO454_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0933076.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4046..4252
                     /locus_tag="RO454_001212"
     CDS             4046..4252
                     /locus_tag="RO454_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HDY0933077.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4328..4624
                     /locus_tag="RO454_001213"
     CDS             4328..4624
                     /locus_tag="RO454_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDY0933078.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHHVDIDSEV"
     gene            4630..5415
                     /gene="bet"
                     /locus_tag="RO454_001214"
     CDS             4630..5415
                     /gene="bet"
                     /locus_tag="RO454_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDY0933079.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            5412..6092
                     /locus_tag="RO454_001215"
     CDS             5412..6092
                     /locus_tag="RO454_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDY0933080.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6089..6271
                     /locus_tag="RO454_001216"
     CDS             6089..6271
                     /locus_tag="RO454_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDY0933081.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            6244..6435
                     /locus_tag="RO454_001217"
     CDS             6244..6435
                     /locus_tag="RO454_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDY0933082.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            6446..6727
                     /locus_tag="RO454_001218"
     CDS             6446..6727
                     /locus_tag="RO454_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDY0933083.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            6826..7047
                     /locus_tag="RO454_001219"
     CDS             6826..7047
                     /locus_tag="RO454_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDY0933084.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            7047..7373
                     /locus_tag="RO454_001220"
     CDS             7047..7373
                     /locus_tag="RO454_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDY0933085.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            7357..7596
                     /locus_tag="RO454_001221"
     CDS             7357..7596
                     /locus_tag="RO454_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDY0933086.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            7736..7972
                     /locus_tag="RO454_001222"
     CDS             7736..7972
                     /locus_tag="RO454_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDY0933087.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            7962..9104
                     /locus_tag="RO454_001223"
     CDS             7962..9104
                     /locus_tag="RO454_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDY0933088.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(9218..10468)
                     /gene="icd"
                     /locus_tag="RO454_001224"
     CDS             complement(9218..10468)
                     /gene="icd"
                     /locus_tag="RO454_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDY0933089.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            10640..11293
                     /gene="rluE"
                     /locus_tag="RO454_001225"
     CDS             10640..11293
                     /gene="rluE"
                     /locus_tag="RO454_001225"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDY0933090.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            11303..11764
                     /gene="nudJ"
                     /locus_tag="RO454_001226"
     CDS             11303..11764
                     /gene="nudJ"
                     /locus_tag="RO454_001226"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDY0933091.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            11818..12924
                     /gene="mnmA"
                     /locus_tag="RO454_001227"
     CDS             11818..12924
                     /gene="mnmA"
                     /locus_tag="RO454_001227"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDY0933092.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            12960..13601
                     /gene="hflD"
                     /locus_tag="RO454_001228"
     CDS             12960..13601
                     /gene="hflD"
                     /locus_tag="RO454_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDY0933093.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            13605..14975
                     /gene="purB"
                     /locus_tag="RO454_001229"
     CDS             13605..14975
                     /gene="purB"
                     /locus_tag="RO454_001229"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDY0933094.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            15144..15815
                     /gene="phoP"
                     /locus_tag="RO454_001230"
     CDS             15144..15815
                     /gene="phoP"
                     /locus_tag="RO454_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDY0933095.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            15815..17275
                     /gene="phoQ"
                     /locus_tag="RO454_001231"
     CDS             15815..17275
                     /gene="phoQ"
                     /locus_tag="RO454_001231"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDY0933096.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            17351..18472
                     /gene="roxA"
                     /locus_tag="RO454_001232"
                     /gene_synonym="ycfD"
     CDS             17351..18472
                     /gene="roxA"
                     /locus_tag="RO454_001232"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDY0933097.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18521..19747)
                     /gene="pepT"
                     /locus_tag="RO454_001233"
     CDS             complement(18521..19747)
                     /gene="pepT"
                     /locus_tag="RO454_001233"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDY0933098.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            19997..21133
                     /gene="potA"
                     /locus_tag="RO454_001234"
     CDS             19997..21133
                     /gene="potA"
                     /locus_tag="RO454_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDY0933099.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            21117..21974
                     /gene="potB"
                     /locus_tag="RO454_001235"
     CDS             21117..21974
                     /gene="potB"
                     /locus_tag="RO454_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDY0933100.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFIFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            21971..22765
                     /gene="potC"
                     /locus_tag="RO454_001236"
     CDS             21971..22765
                     /gene="potC"
                     /locus_tag="RO454_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDY0933101.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            22762..23808
                     /gene="potD"
                     /locus_tag="RO454_001237"
     CDS             22762..23808
                     /gene="potD"
                     /locus_tag="RO454_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDY0933102.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            23866..24327
                     /locus_tag="RO454_001238"
     CDS             23866..24327
                     /locus_tag="RO454_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDY0933103.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            24324..25112
                     /locus_tag="RO454_001239"
     CDS             24324..25112
                     /locus_tag="RO454_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDY0933104.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(25250..26071)
                     /gene="cobB"
                     /locus_tag="RO454_001240"
     CDS             complement(25250..26071)
                     /gene="cobB"
                     /locus_tag="RO454_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDY0933105.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(26087..26998)
                     /gene="nagK"
                     /locus_tag="RO454_001241"
     CDS             complement(26087..26998)
                     /gene="nagK"
                     /locus_tag="RO454_001241"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDY0933106.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(27027..28271)
                     /gene="lolE"
                     /locus_tag="RO454_001242"
     CDS             complement(27027..28271)
                     /gene="lolE"
                     /locus_tag="RO454_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDY0933107.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(28271..28972)
                     /gene="lolD"
                     /locus_tag="RO454_001243"
     CDS             complement(28271..28972)
                     /gene="lolD"
                     /locus_tag="RO454_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDY0933108.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(28965..30164)
                     /gene="lolC"
                     /locus_tag="RO454_001244"
     CDS             complement(28965..30164)
                     /gene="lolC"
                     /locus_tag="RO454_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDY0933109.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            30425..31498
                     /locus_tag="RO454_001245"
     CDS             30425..31498
                     /locus_tag="RO454_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDY0933110.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            31625..35071
                     /gene="mfd"
                     /locus_tag="RO454_001246"
     CDS             31625..35071
                     /gene="mfd"
                     /locus_tag="RO454_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDY0933111.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            35214..36176
                     /gene="ldtC"
                     /locus_tag="RO454_001247"
     CDS             35214..36176
                     /gene="ldtC"
                     /locus_tag="RO454_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDY0933112.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(36259..36516)
                     /gene="bhsA"
                     /locus_tag="RO454_001248"
     CDS             complement(36259..36516)
                     /gene="bhsA"
                     /locus_tag="RO454_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDY0933113.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            36757..37389
                     /gene="comR"
                     /locus_tag="RO454_001249"
     CDS             36757..37389
                     /gene="comR"
                     /locus_tag="RO454_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDY0933114.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(37451..37990)
                     /locus_tag="RO454_001250"
     CDS             complement(37451..37990)
                     /locus_tag="RO454_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDY0933115.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(38217..39521)
                     /gene="ndh"
                     /locus_tag="RO454_001251"
     CDS             complement(38217..39521)
                     /gene="ndh"
                     /locus_tag="RO454_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDY0933116.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         9882 a        10096 c         9839 g         9712 t
ORIGIN      
        1 agaaaaaatt tgttgctcac gcaatcaatc atttcgggcg ttacaactag tcctagatta
       61 tattcgctag gtatacttta tttttaagct aatactttag ctctatttca tcgctccttt
      121 cagcccgaac ttagctttga tttctgcgat cttcgccaga gcctgtgcac gatttagagg
      181 cctaccgccc atgacaggaa gttgttttac tggttcaggt atagcctcac cacggttaat
      241 tcgcgcggtc atacaggcca gttcatcggc agccttgcgc cgtaattccg cgtcagtcaa
      301 cgcattggcc cgcatgttct gatacaggtt ggtaaccagc cagtagtgcg cgtttgattt
      361 ccacggataa gactctgcat ccggatacag accacgcttc cggcaatact cgtaaaccat
      421 atcaaccagc tcgctgacgt ttggcagccc ggcggtaacg gatgcttctt cccggcacca
      481 tgcaacaaac tgcccgggtg atggcagaaa tggtctattc tgccgacggg caacacgcat
      541 tccgtcgctc acctgctcca tcgtggtgat cccgttttcc cggaaagcca gaacccactg
      601 gcggcggatt tcgttcagtt cgttctggtc acgattagcc aggctcgccg ggaaagttgc
      661 cagtaactgg ctgaatacac cgttgattat ctgcgctacc tgctgtacct gcggcttttc
      721 gtcgtactgt tccggcatgt tgttggcgat ccgacgcatc tgctcacggt caaagttaat
      781 catctgtgcg gcgatgtttt tcatagatcc accccgtaaa tccagtctgt gtttgtcagg
      841 tcgagttttg gtttgctagc tgtcacgcct gcctgttgct tgttacggtt gatttcgagt
      901 tgggtccact tgtcgcggag tttggccgga cttagcacgt taccggacca gaagttgtcc
      961 tggcatgccc agcggaacag cacgcacatg tcgcggtggt tacgtccgtc acgttcacgc
     1021 atcaggcgga tatcgttagc ccaccctgca aaattcggtt ttctggctga tggcgcgatg
     1081 gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt cttctgctgt cccccacttg
     1141 ctgccgctct gaattgcagc atccggtttc accacaggaa gatcgttttc tggttggtca
     1201 gaggattcgc cagaattctc ggacgaaaaa ggttttatat tgtcttttgt tagtttgtct
     1261 tttgtgttta cctgattcgg gtaaacgcct ttacctgatt tgggtaaact ttttttacct
     1321 gattcaggta aatttacctc tttcaggtaa actttatttt tcttacctga ttcgggtaat
     1381 gttgaccatt cactgaccac attattaatg ccggtattcc gcccgctctg aataaaaatc
     1441 ccacgcttta ccagaacact ttttgcagca gaacacttgt gcggcaatat cccggttaat
     1501 tcggaaagtt gctcgttgct aacccaatcc agttttttat taaagccata tgttttgcgc
     1561 atgacagcca gaaagaccag aagctggtgc tgtgttaatc cggccagcat cacagcttcc
     1621 agcaactcat ttgcaatgcg cgtataacca tcatcgagat ctgccacgcg cggctccttt
     1681 tgtgccgcat ccggcactgg aaaattgaat atctcagcag tgtttgccat aattcctccc
     1741 gcaatgagtg tgttacgatt tgcacctgaa agtcggttct gttccagcag accggctttc
     1801 gccatttctg aacctgtcat atcgccccca gcatggtagt aaccatcgcc atcaatggac
     1861 cagccagatc tgggtccaca cgaaacatcg acacaatacc ttcactaatt tccttcagtt
     1921 tctggtggcg tggtgcgttg agaatgacag cctgttttgc ctcactgagt tccttttcca
     1981 tttcagccaa cctagccatg aagctatcct gctcaaccag gtaaccgcga tattccagcg
     2041 gtagtaccgc cagaattgcc ggggtcagtt cacgcacgtt atttcggtat ttttcagaat
     2101 cgaatttgtt atcgaggaag cggaacagct tctggcgtgc acggctgaca tcatcaggga
     2161 aatcgatggt gccgccgccc tgctccagat actcattcac aatgagtgtg gcaacgacat
     2221 cctgattatc tacagccgac caggcgcgga cggcatcacg gattttttcg tggcctggca
     2281 cctgttttgt ttgagaacga tttatcaccg cagtcgggct aaatccgcta gtctgttggt
     2341 atgtaagtgg ttgcataatt gactccttta gtttgaattg actgttaagt tgattgctta
     2401 ttgttaaaga gcgtgaaatg gaaatttaag ctgcgttctt ttcggtgtgt ggaaacaact
     2461 tcggaagatc cgggcgaatc tggtatgcct tcactactcc accagtagcc gtaacaatgc
     2521 tgccgacatg ttcaggggat acctttgctt tgttgtgaag ccacttatag acggcctgct
     2581 gtgaaacttc gcaagcagcg cccagtttct tttgtgaacc aacgatattg atcgctgttt
     2641 tgatagctgg gttcataaca acctccgtgg ttaatttgaa tcaagattaa aactatggtt
     2701 gtttttagtc aacaaccatt ttcgtttgat ggaataaaac cttggttgta catttggact
     2761 atgaaaacaa cactctcaga aagacttaaa gaagccagat tagcgcgagg ccttacacaa
     2821 aaggcgcttg gggatttggt cggggttagc caggctgcta ttcagaaaat cgaaacaggg
     2881 aaagctaatc aaacaactaa aatcgtggag atcgcgaacg ctttgggtgt gcgcgcagaa
     2941 tggttatctt ctggcgttgg aaatatgtca gacagtacag tgcaaccaat acaatcaact
     3001 gtcagccatt ccaaatactt caagattgac gttcttgata tagaagtcag tgctgggccg
     3061 ggagtcatca accgtgagtt tgtagaagtt ctacgctcgg ttgagtactc gtttgacgat
     3121 gctcgtcaca tgttcgatgg taggaaggcg gaaaatatcc gcatcattaa cgtgcgtggt
     3181 gacagcatgt caggaacgat cgaaccaggt gatctgctgt tcgttgatat cacagttaaa
     3241 tctttcgacg gtgatggtat ctatgcgttt ctgtacgacg acacagccca tgtaaagcgc
     3301 ctgcaaatga tgaaggataa gctgctggtc atctctgata acaaaagcta ctcaccgtgg
     3361 gacccgatcg agaaagacga gatgaaccgg gtgttcatct tcggtaaggt tattgggagc
     3421 atgccgcaga catataggaa gcatgggtag taccaattaa aaattatcaa ctgggcattg
     3481 tgctcattca gtaaagaact aattcctatc tttgctctag gtagtaatat taagccaccg
     3541 caataatatc tttacctaac ggcgtaagaa tcccggtcac cgtgccgggt tttcttttgc
     3601 cctcccctca tcacacacac cgttaaaaaa accaccataa cctcgcttca gttatcgcta
     3661 tgcgattcaa gtcacaaaat aaatccatcc taaatacaac cagttatatc taaaacaacc
     3721 aataaaacaa cttttgttgt tgacgataaa acaactatag ttttaaataa gttcatcgca
     3781 acaacacaac gatacggcaa ctacctgatt caccgttgcg atgaccgctt agatccgcag
     3841 tttgaatttc agcaggcttc ggggagtgcg aggggtgaaa cggacgcgtg aacgtcggtg
     3901 tgaccagctg aaattaactc aacatttcat accttagtcg cttcaacgag gcggcttagt
     3961 tatgacaacc ggcggccatc caccgcctga atacgcgcag aagtctctat atgttcagca
     4021 gcccagctta cgggcaggag tttttatggt tcatcaacat tacggaacgc agaccgttaa
     4081 tcgcggtgcg gtcatgccag gaatgctggt caaacacaaa gatggtacct ggactgcatc
     4141 agctaattta cgcggacggc tttatctgca tcgcggcatc gagcgcactt atacccgtga
     4201 tttgctcgtg gaagtttttc tcgacggacg cggcaacggc ctgaatcact aatccccttt
     4261 cctgtttacc taatcagccc ggcatttcgc aggcgatatt ttcacagcca ttttcaggag
     4321 ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcaaagct gggcgcgtca
     4381 ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
     4441 tcttccacag cacctgtttg aatcactctg catcgatcat ttgcaacgct gcggggccag
     4501 caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
     4561 acacatccgg tacatggttg aaaccattgc tcaccaccat gttgatattg attcagaggt
     4621 ataaaacgga tgagtacagc actcgcaacg ctggctggga agctggctga acgtgtcggc
     4681 atggattctg tcgacccaca ggaactgatc accactcttc gtcagacggc atttaaaggt
     4741 gatgccagcg atgcgcagtt catcgcattg ctgatcgtcg ccaaccagta cggccttaat
     4801 ccgtggacga aagaaattta cgccttccct gataagcaga acggcatcgt tccggtggtg
     4861 ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt
     4921 gagcaggaca atgaatcatg tacgtgccgg atttaccgca aagaccgcaa tcatccgatc
     4981 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga
     5041 gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacggcataa agccatgatt
     5101 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     5161 attgtcgaaa atactgcata cactgcggaa cgtcagccgg aacgcgacat cactccggtt
     5221 aacgatgaaa ccatgcagga gattaacact ctgctgattg ccctggacaa aacatgggat
     5281 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgcgc atcgtcagaa
     5341 ctgacacagg ccgaagcagt gaaagctctt ggattcctga aacagaaagc cactgagcag
     5401 aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgac gtgagagctg
     5461 tcgaacaggg ggatgatgca tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     5521 ttcacaacgt gatagcaaag ccccgctcag gaaagaagtg gcctgacatg aaaatgtcct
     5581 acttccacac cctgctggct gaggtttgca ccggtgtggc tccggaagtt aatgctaagg
     5641 cgctggcgtg gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg
     5701 gcgtgaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgc accgcctgct
     5761 ctcccgatgg tttatgcagt gacggcaatg gccttgagct gaaatgcccg tttacctccc
     5821 gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaagtcggct tacatggccc
     5881 aggtgcagta cagcatgtgg gtgacacgaa aagatgcctg gtactttgcc aactatgacc
     5941 cacgaatgaa gcgtgaaggc ctgcattatg tcgtggttga gcgggatgaa aagtacatgg
     6001 cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaagca ctggctgaaa
     6061 ttggttttgt atttggagag caatggcgat gacccatcct cacgataata tccgggtagg
     6121 cgcgatcact ttcgtctact ccgttacaaa gcgaggctgg gtatttcacg gtctttctgt
     6181 tatccgaaat ccgctgaaag cccagcggct ggctgaggag ataaataata aacggggggc
     6241 ggtatgcaca aagcatctcc tgttgagtta agaacgagta ttgatttggc acatagcctt
     6301 gctcaaattg gagtcaggtt tgtgccaata ccagcagaaa cagacgaaga atttcatacg
     6361 ttagccacat ccctttcaca aaagctggaa atgatggtgg cgaaagcaga agcagatgag
     6421 agagaccagg tatgacaacc actgaatgca tttttctggc agcgggcttc atattctgtg
     6481 tgcttatgct tgccgacatg gggcttgttc agtgacacct cagcaagaaa atgcccttcg
     6541 cagcattgcc cgtcaggcta attctgaaat caaaaaagtc agacagcagt ttccggataa
     6601 aaacgtcaat gacatttgcc gtagcgtact gaagaagcac cgcgaaacgg taacgctgaa
     6661 gggattcaca ccgactcatt taagcctggc aatcggcatg ttaaacggcg tctttaagga
     6721 acgatgaaca tgaaaagcaa aattatcagg gagctacagg ctcctttttt attgttcgca
     6781 ttcaccctca agcgtattaa ccaacagttc agggattaat gaaagatggc agacatcatt
     6841 gattcagcat cagaaataga agaattacag cgcaacacag caataaaaat gcgccgcctg
     6901 aactacctgg ctgtatctgc cactcattgt tgtgagtgtg gcgatccgat agatgaacga
     6961 agacgcctgg ccgttcaggg ttgtcggact tgcgccagtt gccagcaaga tctggagctt
     7021 atccgcaaac aaagggggat taagtgatgg ctaacctgca acttgctgtt aaaagtgaat
     7081 acttcgatgc catgattcgc ggagagaaaa cggaagagta tcgcttgtgt aatgactact
     7141 ggaataagcg aattatgttc cgggaatatg accgcctgat tatcacaaag ggatatccga
     7201 agcgcgacga ttccagtcgc agaattgatg ttccgtatga cggatatgaa gtgaagacaa
     7261 tcacacatcc gcactttggc gataaaccgg taaaggtgtt cgctataaag gtgaatatca
     7321 gcactgaata tcaatccgca caacacaagg tcaagaatgt tcagagtgat tgaccctaac
     7381 acctggtacg tcgaccacca cggcactccc tgcaaaatcc tgcgttctac ccacaacaaa
     7441 gttcactaca tccgaaaagg cagaacatgt atcgccagca tgttccgctt taatcatgac
     7501 tttgaacctg tgaataaagc tgatgcagat cggatagcag aagagatcga aacggcagaa
     7561 cacattaaga agttacgtga catgcgcagg aaatagaaaa attgataaat tcaatactgc
     7621 atttctcagc attaaattta tctctatgac cagtcaagag atgtacctgc catgagctta
     7681 atatcatgtc agatatatcg gtcacaaact ccctcagcag ctaagaggag gacaaatgtc
     7741 tcgactaatc actttacagg actgggctaa agaagaattt ggggacttag caccaagtga
     7801 gcgagttctg aaaaaatacg cgcaagggaa aatgatggcc ccacccgcta taaaagttgg
     7861 tcgctactgg atgattgacc gaaattcccg ttttgtagga acgcttgcag aaccgcaact
     7921 cccaataaac gcaaacccaa aactccaaag gataatcgct gatggctgct agaccccgat
     7981 ctcacaaaat ctctataccc aatttatatt gcaaattaga taagcgaacc ggaaaggtat
     8041 attggcaata caaacatcca ctatccggtc gttttcatag cttaggaact gatgagaatg
     8101 aagcaaaaca agttgctact gaagcaaata ccattattgc tgaacaacgt accagacaaa
     8161 tattaagcgt caatgagcgt ctggaaagaa tgaaaggcag gcgctcagac attacggtga
     8221 cagaatggct tgataaatat atttctatcc aagaggacag gctgcaacat aatgaactaa
     8281 gacccaactc ctatcggcaa aaaggcaaac ccattcgtct tttccgtgag cattgtggaa
     8341 tgcaacacct caaggatatt accgcacttg atattgccga aataattgat gctgtaaagg
     8401 ctgaaggtca taacaggatg gcgcaagtcg tgagaatggt gttgatcgac gtcttcaaag
     8461 aagcacaaca cgcaggacat gttccgccag gatttaaccc agcgcaggca acaaaacaac
     8521 cgcgaaatcg agtaaaccgc caaagattgt cactgcccga atggcaggca atatttgaca
     8581 gcgtaagcag acggcagccc tatttaaaat gcggcatgct acttgctctt gttactggac
     8641 aacgtttagg cgatatctgc aatttgaaat tctctgatat atgggacgac atgttgcaca
     8701 ttactcagga aaaaaccggt tcaaaacttg ctattccgct taacctgaaa tgcgatgctc
     8761 tgaatattac ccttcgtgaa gttatatctc agtgcaggga tgctgttgtt agtaaatatc
     8821 tggtccatta ccgtcacact acctctcaag caaacagagg agaccaggtt tctgcaaata
     8881 ctctgacaac ggcttttaaa aaggcccggg aaaaatgtgg cataaaatgg gagcaaggaa
     8941 ctgcgcccac atttcatgag cagcgatctc tgtcagaacg gttatatcgg gaacagggtc
     9001 tggatacgca aaagttgtta ggccataaat ccagaaaaat gaccgaccga tacaatgatg
     9061 atcgtggtaa agactggatt atcgtagata tcaaaacagc atagaaaata gccagttttg
     9121 gggaagggtt ttggggaaag ttttggggaa gattttacat catcataaaa caacgggcgt
     9181 gttatacgcc cgtttcaata tttaacacat gtagtgatta catattcttg atgatcgcgt
     9241 caccaaactc tgaacatttc agcagtttag cgccttccat cagacgttcg aagtcatagg
     9301 ttacggtctt ggcattgatt gcgccttcca tacctttaac aatcaggtca gccgcttcag
     9361 tccaacccat atggcgcagc atcatctcag cggagagaat aatagagcct gggtttactt
     9421 tgtcctgtcc ggcatatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt
     9481 caccgatgtt tgcgccaggg gcgataccga taccaccaac ctgcgctgcc agggcgtcag
     9541 aaatgtagtc accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca
     9601 ggatctgttg caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat
     9661 tcgggttttt aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac
     9721 gcgccagttg gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt
     9781 tgtgcaccag ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa
     9841 ccagacgttt ggtgccttct tccgaacacg gcttaatacc gataccgcaa tgttccggga
     9901 agcgaatttt cttcacgccc atctcttcac gcaggaattt aatcactttc tcagcgtcgg
     9961 cagagtctgc tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa
    10021 ccatatcggt cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg
    10081 gacgcaggca gatgtagaga tccagttcct ggcgcagggc aacgttcaga gagcgaatac
    10141 cgccaccaac cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat
    10201 caagggtttc agcaggcaac cagacgtcct gaccataaac ctgtgtggat ttttcaccgg
    10261 tgtaaatttc catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt
    10321 cgaccacttt cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag
    10381 ggataatcgg attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt
    10441 gtgccggaac aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc
    10501 gtctgccacc acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt
    10561 tttgttaatg atttgtaatt agcttgtcaa tactacccta ttgtttgccg tcatgaaaga
    10621 tgcgcgttat taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac
    10681 ttcttttaga aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaacc
    10741 tgacaaccag cccacgcgtg tgatcctgtt caataaaccc tacgatgttc ttccgcagtt
    10801 caccgatgaa gccggacgca aaacattaaa agaattcatc ccggttcagg gggtgtatgc
    10861 ggcaggtcgt ctcgaccgcg acagcgaagg gttactggtg ctgaccaata acggcgcgtt
    10921 gcaggcgcgt ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga
    10981 aggtattccc acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg
    11041 ccctaccctg cccgcaggcg cggagctggt tgaagaaccc gcgtggttat ggccgcggaa
    11101 tccaccaatt cgtgagcgca aaagtattcc caccagctgg ctgaagatca ccttatatga
    11161 aggacgtaat cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact
    11221 gattcgctat gcgatggggg attactcttt ggataatctt gccaatggcg aatggcgaga
    11281 cgcgacagat taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca
    11341 cgcagaaggc aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca
    11401 acctgccggg cacctggaag ccgatgaaac cttagtggaa gccgccgccc gtgagctgtg
    11461 ggaagaaacc ggcattagcg cgcagccgca acactttatt cgtatgcatc agtggattgc
    11521 gcccgataaa acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc
    11581 gacgcagcct catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt
    11641 acaggcgtca aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg
    11701 gcaacgctat ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat
    11761 ctaaaaggct gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg
    11821 tctgaaaccg caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct
    11881 gcctggctgt tacaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa
    11941 gaagacgacg gtgaggaata ttgcacagcg gcagcggatc tggctgatgc ccaggctgtc
    12001 tgcgacaagc tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac
    12061 gtcttcgaac tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg
    12121 tgcaacaaag agatcaaatt taaagctttc ctcgaatttg ccgccgaaga tttaggtgcc
    12181 gattatatcg ctaccggtca ttacgtacgt cgggcagatg tcgatggcaa gagccgcctg
    12241 ctgcgtggtc tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag
    12301 cagattgcgc aaagcctgtt cccggtcggc gaactggaaa aaccgcaggt gcgtaagatt
    12361 gctgaagatc ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt
    12421 ggcgaacgta aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc
    12481 attaccgtcg atggcgatga aattggtgag caccaggggc tgatgtatca caccctcggt
    12541 cagcgtaaag gtctgggtat cggtggcacc aaagacggca ccgaagaacc gtggtatgtg
    12601 gtcgataaag acgtcgaaaa caacattctg attgttgcgc aaggccatga acacccgcgt
    12661 ctgatgtctg tcggtctgat tgcccagcag ctgcactggg tcgatcgcga accgttcacc
    12721 ggtactatgc gttgcacagt aaaaacccgc tatcgccaga ccgacatccc gtgcaccgtc
    12781 aaggcgctgg acgatgatcg cattgaagtg attttcgatg agccggttgc cgccgtgacg
    12841 ccgggccagt ctgccgtttt ctataacggt gaagtgtgcc tcggtggcgg tattattgag
    12901 cagcgtctgc cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg
    12961 tggcaaagaa ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc
    13021 tggtgcaaca actcgctcac caggggcatt gtgatggcga tgcgctacac gtctcactca
    13081 acagtattat cgatatgaac cccagctcaa cgctggcggt ttttggcggc agcgaagcca
    13141 acctgcgcgt cgggctggaa accttgctcg gcgtactcaa tgccagcagt cgccaaggct
    13201 taaacgccga attaacccgc tacacactca gtctgatggt gcttgagcgc aaactctcct
    13261 cagcgaaagg tgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg
    13321 aacacttcga tttacagtcc gaaacgctga tgagcgcgat ggctgccatc tatgttgatg
    13381 ttatcagccc gcttggcccg cgcattcagg tcacgggttc ccctgctgta ctgcaaagcc
    13441 cacaggtgca ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct
    13501 ggcaccaggt cggcggcggt cgtctgcaac tgatgttttc tcgtaatcgc ctgaccactc
    13561 aggcaaaaca aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac
    13621 tgaccgccgt ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga
    13681 ttttcagcga atacggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa
    13741 aactggccgc gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt
    13801 accttgatgc aattgtcgcc agtttcagcg aagaagatgc agcacgcatc aaaaccatcg
    13861 agcgtaccac taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg
    13921 atatcccgga actgcacgcg gtttctgaat tcatccattt tgcctgtact tcggaagata
    13981 tcaataacct ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt
    14041 actggcgtca actgattgat ggcattaaag atctcgccgt tcagtatcgc gatatcccgc
    14101 tgctgtcccg tacccacggt cagccagcca cgccgtcaac cattggtaaa gagatggcta
    14161 acgtcgccta ccgtatggag cgccagtacc gccagcttaa ccaggtggag atcctcggca
    14221 aaatcaacgg cgctgtcggt aactacaacg cccacatcgc cgcttacccg gaagttgact
    14281 ggcatcagtt cagcgaagag ttcgtcacct cgctgggtat tcagtggaac ccgtacacca
    14341 cccagatcga accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca
    14401 ccattctgat cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac
    14461 agaaaaccat tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg
    14521 acttcgaaaa ctccgaaggg aacctgggcc tttccaacgc ggtattgcag cacctggcaa
    14581 gcaaactgcc agtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc
    14641 tcggcgtggg tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca
    14701 aactggaagt gaaccatgac catctgctgg aggagctgga tcacaactgg gaagtgctgg
    14761 ctgagccaat ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga
    14821 aagagctgac tcgcggtaag cgcgttgacg ccgaaggcat gaagcaattt atcgacggtc
    14881 tcgcgctgcc agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg
    14941 gtcgcgccat caccatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga
    15001 tgctgtccgg cctgcttatt aagattatcc gctttttatt ttttcacttt acctcccctt
    15061 cccgctggtt tatttaatgt ttacccccat aaccacataa tcgcgttaca ctattttaat
    15121 aattaagaca gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac
    15181 gtcaccatct taaagttcag atacaggatg ctggtcatca ggtcgatgac gcagaagatg
    15241 ccaaagaagc cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg
    15301 gattgcctga cgaggatggt ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac
    15361 tgccgattct ggtattaacc gcccgtgaga gctggcagga caaagtcgaa gtattaagtg
    15421 ccggtgctga tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc
    15481 aggcattaat gcggcgtaat agcggtctgg cttcacaggt catttcgcta ccaccgtttc
    15541 aggttgatct ctcccgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt
    15601 tcgaatacac cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt
    15661 cgttaatgct acaactctac ccggatgcgg agctgcggga aagccatacc attgatgtac
    15721 tgatgggacg tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtg attaccaccg
    15781 ttcgcggcca gggctatctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt
    15841 cccgctctcg ctgcgggtac gttttctgtt agcaacggca gcagtagtat tggtgctttc
    15901 gcttgcctac ggaatggttg cgctgatcgg ttatagcgtc agtttcgata aaaccacgtt
    15961 tcggctgtta cgtggcgaga gcaatctgtt ctataccctt gcgaagtggg aaaacaataa
    16021 gttgcatgtc gagttacccg aaaatatcga caagcaaagc cccacgatga cactaattta
    16081 tgatgagaac gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaagatgat
    16141 ccagcccgac tggctaaaat cgaacggttt tcatgaaatt gaagcggatg ttaacgatac
    16201 cagcctcttg ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga
    16261 tgatgacgac gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg
    16321 gatgccaaag ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta
    16381 tatggtctgg agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct
    16441 gctgtgggtc gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg
    16501 cgaactggaa gaacataacc gcgaattgct caatccagcc acaacgcggg aactgactag
    16561 tctggtacga aacctgaacc gactgttaaa aagtgaacgc gaacgttacg acaaatatcg
    16621 tacgacgctc accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac
    16681 gctgcgttct ctgcgtagtg agaagatgag cgtcagtgat gctgagccgg taatgctgga
    16741 gcaaatcagc cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg
    16801 cgggacattg cttagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc
    16861 ggcgctgaac aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga
    16921 gatcagcttt gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa
    16981 tgcctgtaaa tattgcctcg agtttgtcga aatatctgca aggcaaacag acgagcatct
    17041 ctatattgtg gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt
    17101 cgaccgtggt caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc
    17161 ccgcgaaatc accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg
    17221 tgcgcggatg gaggtgattt ttggtcgcca gcattctgca ccgaaagatg aataaatatg
    17281 tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg
    17341 gatgcttaac atggaatacc aactcactct taactggccc gattttcttg aacgtcactg
    17401 gcagaaacgt ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc
    17461 agacgagttg gcgggtttgg cgatggaaag cgaagtcgac agtcgactgg tcagtcatca
    17521 ggatggcaaa tggcaggtca gccacggtcc attcgaaagc tacgatcatc tcggtgaaac
    17581 taactggtca ttgttagtgc aagcagtgaa ccactggcat gagccgaccg ccgcgctaat
    17641 gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgatct ctttttctgt
    17701 accgggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac
    17761 cggtcgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gccctcaccc
    17821 ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggcga
    17881 tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa
    17941 ctattccgtg ggttttcgcg cgccaaatac gcgggaactg attagcggat ttgccgatta
    18001 tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat cctgatgttc cacctcgcgc
    18061 tcatcctgcg gacgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt
    18121 gatcaaccag ccagaacatt ttaagcaatg gtttggcgag tttatatccc agtcacgtca
    18181 tgaactggat atcgcgccgc cagagccgcc ttatcagccg gatgaaatct acgatgcgct
    18241 gaaacaaggt gatgtgctgg tgcgcctggg gggtctgcgc gtattgcgca ttggcgacga
    18301 cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcgctcgc
    18361 cagcaacatt gcgctgaccg cggagaattt tggcgatgcg ctggaagatc cgtcattcct
    18421 cgcgatgctc gcggcgctgg tcaatagcgg atactggttc ttcgaagggt aagtttggat
    18481 ttaagccgga tgcggcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtca
    18541 actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga gtcacaaact
    18601 catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcacggt aatcccataa
    18661 acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc
    18721 agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc
    18781 gcatattgta gtaactgtct tcaatcacca gttcaatgta gcaatcagga tgtaaccctt
    18841 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
    18901 cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca
    18961 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
    19021 catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccgggt
    19081 gaacattgtt accgacaatt ttgatattga ccgacgcggc gttgaagttt tcaaactcca
    19141 gttcacctac gccaccacca tcaacggtgt aagcccagcg ggcatcgaag gcgtcaacat
    19201 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
    19261 catgcggaat gtttttttgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
    19321 ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcagcgtc tgacccagta
    19381 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
    19441 tatcgccacc gcgatagttt tcaacaattt gcggattcac atttttgccg ctgcaatccg
    19501 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg
    19561 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatctctt
    19621 cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
    19681 tcacccctgc ctttgattgg gtatcaagag acacgtagtt caaaaatcgc tcaagtagtt
    19741 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
    19801 tattgcgtca ggtcactttt agccctgcaa gcgggaatat ttatcagcat taccttaatg
    19861 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
    19921 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
    19981 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
    20041 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
    20101 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
    20161 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
    20221 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt
    20281 ccaaagctac gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
    20341 catgcaaaaa acccccgcag ctgaaattac gccccgcgtg atggaagccc tgcggatggt
    20401 gcagctggaa accttcgctc aacgcaaacc gcatcagctc tctggtggtc aacagcaacg
    20461 cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
    20521 ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg
    20581 taagcttggc attacattcg tgttcgtaac tcacgaccag gaagaagcac ttaccatgtc
    20641 ggacaggatc gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat
    20701 ctacgaagag ccgaaaaacc tgtttgttgc cgggtttatt ggcgaaatca atatgtttaa
    20761 cgccactgtc atcgaacgtc tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga
    20821 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
    20881 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgccgaag ggctgattgg
    20941 ttacgttcgc gagcgtaact acaaaggcat gacgctggag tcggttgttg agctggaaaa
    21001 tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc
    21061 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
    21121 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
    21181 ttatctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
    21241 gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
    21301 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
    21361 tgggctatcc ttttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
    21421 tgtttctgct gattgttccg ttctggacca actcattgat tcgtatttac gggctgaaaa
    21481 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgata
    21541 caccaattcg catcatgttc acgcctagtg cggtgattat cggtctggtt tacattctgc
    21601 tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg
    21661 aggcggcgcg cgatctcggt gccagcaagt tgcagacctt tattcgcatc attatcccac
    21721 tgacgatgcc gggaattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
    21781 tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg
    21841 tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta
    21901 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg aataagaagg
    21961 tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta
    22021 cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat
    22081 caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg acagcctgtt
    22141 acaggcagcg cagcattcac ttacaatggc ggtgttttcg gcgacgtttg ctacgcttat
    22201 cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgtggtaagc cgttcgttag
    22261 cggaatgctg tttgtggtga tgatgtcacc agatatcgta atggcgattt cgctgctggt
    22321 gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac
    22381 cttctgcctg ccatttgtgg tagtgacagt gtattcgcgc ctgaaaggtt ttgacgtgcg
    22441 aatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc gaaaaatcat
    22501 tctgccactg gcaatgccag cggtagcggc gggctgggtg ttaagcttta ccctgtcgat
    22561 ggacgacgtg gtggtttctt cgtttgtcac cgggccgagt tatgaaattc tgccgttaaa
    22621 aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt
    22681 actggtgctg tcgctggtga tggtaattgc cagccagctt attgctcgtg ataaaacgaa
    22741 aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct
    22801 ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta tttctacaac
    22861 tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag
    22921 gttatctatt cgacttacga gtcgaacgaa accatgtacg cgaagctgaa aacgtacaaa
    22981 gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagaa
    23041 gggatgatcc agaagatcga caagtcgaag ttaagcaatt tcagcaacct cgatccagac
    23101 atgctcaaca agccgtttga cccgaataac gactattcca ttccgtatat ctggggtgcg
    23161 acggcgattg gcgttaacgg tgatgcggtg gatccgaaat ctgtcaccag ctgggccgat
    23221 ctgtggaagc ctgagtacaa aggcagcctg ctgctgaccg acgatgcccg tgaagtgttc
    23281 cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa agagattgaa
    23341 gctgcataca acgagctgaa aaagctgatg ccaaacgtcg cggcgtttaa ctccgataac
    23401 ccggcgaacc cgtatatgga aggcgaagtt aacctcggca tgatctggaa cggttctgct
    23461 ttcgttgcac gccaggcggg tacgccaatt gatgtggtgt ggccgaaaga aggcggtatt
    23521 ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa
    23581 ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac tatcggttat
    23641 ccaacgccaa accttgcggc gcgtaagctg ttaagtccgg aagtggcgaa cgataaaacg
    23701 ctctacccgg atgctgaaac cattaaaaat ggtgaatggc agaatgacgt tggcgcagcc
    23761 agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt cagcaagcgg
    23821 gcggtgatat taccgcccgt tttttattta gcaaggatgt ggctaatgag ccaggacagt
    23881 agagttttct ttcgtatatt cttcggtatc ggtctggtac tgatcctcat ttctgttgtt
    23941 atcttttata accagttcac ctatagcaaa gacgcgattc atactgaagg cgtcattgtt
    24001 gataccgtct ggcactccag tcattctcat cgcacgggta aaaatggttc gtggtatccc
    24061 gtcgttgcct ttcgtccgac gccagattac acgctaattt tcaattcgag tattggcagc
    24121 gatttttatg aaggcagcga aggcgataaa gttaatgttt attattctcc aggacatcct
    24181 gaaaaggctg agataaataa cccgtgggtt aattttttta aatgggggtt tatcggcatt
    24241 atgggcgtta tttttatctc cgtcggttta ttaatcagta tgccctcctc taaaaagaca
    24301 cgcaggaaac ggaagtcccg cccatgaaaa aaatcataat attgctcagt ttactgatac
    24361 tcctgcctct gacggcaacc agtaagccct taattccaat aatgaagaca ctttttacgg
    24421 atgttaccgg tactgtacca gatgctgaag agatcgcgca taaagccgag ctttttcgcc
    24481 aacaaaccgg agttgcaccg tttattgtgg ttttacccga cattaataat gaagccagtc
    24541 tcaggcaaaa tggcaaagcg atgctggcgc atgcggcatc ttcaatgagt aatgtaaaag
    24601 gaagtgttct gttactcttt actacccgcg agccacggtt aattatgata accaacggcc
    24661 aggttgaaag cggtctggac gataaacatc tcggccttct ggtagaaaat cacacgctgg
    24721 cttatttaca tgcggacctc tggtatcagg gtatcaataa tgcattggct gttttacaag
    24781 cacagatatt aaaacaaccg acgccaccac tgacatatta tccgcatccc ggacaacagc
    24841 atgaaaatga tccacccggc agtaccacga cattaggcct ttttgcctgg gcagttgcat
    24901 tcatcgtctt tgctgcattt tttaactaca caacgcgcct ttattatgcg ttaaaatttg
    24961 cagtggcgat ggctgttgcc aatatgggtt atcaggcttt atgtttgtat atcgataaca
    25021 gctttgcgat taccagaata tcgccactct gggcagggct gattggtgtc tgtacgttta
    25081 ttgccgcgtt actatggacg agtaaacggt aaaagaggca gtaaagttcg cgtgtattat
    25141 gccggatgcg gcgcgaacgc cttatccggc ccacaaaacc cgcaaattca attaattgcg
    25201 ccccttcaca ggcctgacaa gcgtagcgca tcaggcaatt tttcccgctt tataatccct
    25261 tcagcaactt ttcaacaaac tctggcacca cctggcttgc cggaccgtaa tatttctcgg
    25321 caaattcatt accaacctga ctcggttcaa gattcagttc cacggtgtgc gcgccatgca
    25381 gtttcgcttc gtgaacaaac ccagccgccg gataaacatg ccccgaagta ccaatggcaa
    25441 tgaaaatatc ggccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc
    25501 caaaccacac tacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt
    25561 ctggcgtaac gtctccggtc cagtcgagga cctggccact ttgcgaacaa cgtactttca
    25621 gcaattcccc atgcatatga atcacattgg tattgcctgc gcgttcatgc aggttatcga
    25681 tattttgcgt caccagcaaa aagcgatcgc cgagagcatc ttgcagtttc gccagcgcaa
    25741 gatgtgcagc gttaggctga atttctggct gctgcaactg tcgacgacgg gcgttataaa
    25801 acgtttgcac cagttcagga tcgcgatcaa aaccttccgg cgttgccaca tcttcaaccc
    25861 gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa
    25921 tccctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat
    25981 ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac
    26041 gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgattaa tcggttagat
    26101 gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg
    26161 gaacacgagc tacaggtaag agatgacgag gcaacctttc cgccagttgc gttgtgattg
    26221 ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga
    26281 tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct
    26341 catcgccttg atcataaagc gcaatgatct caggagcctg caacggttga tgataatagt
    26401 gttgatacag ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac
    26461 cacagccgca gcggcgtaac gggaaatcca gccccatcat ggttaacgca tcaaccggca
    26521 gacgcatatg gccaaactcg ccggtaatat agcttttacc ggtaattggt ttaccgttga
    26581 aaatcagccc gccgccaacg ccggtgccga gaatcaaccc catcaccagt ggatattgag
    26641 taaattcatc atcccatgct tcagaaaggg caaaacagtt ggcatcgtta tcaaggcgta
    26701 catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg gcagcaggga
    26761 cattggcggc atacagcgta ccatcttctg tttccggcat acccggaata ccaatgccga
    26821 cagaaccttt acagccaaaa cgttggtcag cttcggctac cagctcgcac actgcatcta
    26881 aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac
    26941 cgctatcaaa cacgcctagc gcaatttttg ttccaccaat atcaaacccg taatacattg
    27001 ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata
    27061 ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc
    27121 agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaatagata
    27181 tcgctggaga ggaactgatg gccgatcagc ttttcaatcc actcaataat cggggtaagt
    27241 tgcagtgaaa caactacgcc gataatcaca ccacacaggc tgccgaacag ccctgccagc
    27301 aatccatacc agacaaagat ggcgcggatt aaaccatctt tcgcacccaa cgttcttaat
    27361 actgcgatat cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa
    27421 caggccacac caatcaccag taccatcgcc agatacataa tggcgcggat catctgaata
    27481 tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata gctgttggtc
    27541 acttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca
    27601 atacctgaca cgctggaacc catatcaaga tattgctgag catcggccag cgggatcatg
    27661 gcaaaactgt gatcgagttg accactcaac tgcaaaatac cggcaacgtg caaacgcaca
    27721 cgttttggct gcatcagttt atgctcggga ttcgaatttg ggatcataat cgacacccaa
    27781 tccccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg
    27841 cctgctttaa aattgctcca cgcatcgtcc tggacaaacg agggtaatgc gctcaaacgc
    27901 tgttcctgtt gcgggttaac gcccttgacc tggattgcgc gcagattcgc gccactttcc
    27961 accagcccgg tgaaattgat atacggcgca gcggcggcaa tgcccggcac tttctgcacg
    28021 ttatccagtg cttcctgcca gttggtccac ggctgatcta ccgcctcaat ttcaccgtgc
    28081 ggtactaccg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag
    28141 ccgacgatca ataccgccac gccgagggca atgccaatgg tagaaatgac ggagatcaac
    28201 gacaccatgc cgccgcgtcg ccgtccgcgg ctaaaacgca ggccaatcaa taacgataaa
    28261 ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca
    28321 tttctagttg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg
    28381 cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg gcgttacgtg
    28441 catcgaggtt accagtaggt tcatccgcca gtaccagccg cgggttattg accagcgcac
    28501 gggcaatcgc cacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac
    28561 gatgctccag ccccaccgct tttaacatct ccagcgcacg gctgttgatt tcagcgggct
    28621 ttttcttgcc aatcaacagc ggcatagcca cgttttccag ggcggtaaaa tccggcagca
    28681 ggtggtgaaa ctgataaata aatcccagct tctggttgcg cagttctgct ttcgccgccg
    28741 aagacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt ggcgtatcca
    28801 gcccgcctaa caggtgcagc aaggtgcttt taccggaacc agagctgccg acgatcgcca
    28861 tcatttcacc ttcgcccacg ctgaaactga cattgtgcag tacatcggtt tgcacactgc
    28921 cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa
    28981 gcctcagcgg gttgagtggc ggcagcgcgc caggaagggt aaagcgtaga cagcagcgcg
    29041 atagccatcg ccaccagcgc aataacaatg acctgtaaag gctcgatagc gaccggcagc
    29101 gccgcgccat caagcaggac gccgattatc ggcatcagat tattcaactg gctggcaagc
    29161 agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc cccttgcacc
    29221 ataaagacca tcatgatttg ccgcggagtc agcccctgag tctgaagaat ggccacttcg
    29281 ccctgcttct ccatcaccat cagtcccagc gaggtaataa tgttaaacgc cgcaacggcg
    29341 acaatcaggc tcagcagcaa gcccatcata tttttttcca tgcgtacggc ctggaacagc
    29401 tcgcctttac ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt
    29461 aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat attgcctgcc
    29521 ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact
    29581 tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc
    29641 atcggcgtga actggctagc agatggcacc atcacgcgga tttgatcacc gcgattgacg
    29701 cccaactgtg aggcaagctg ttcaccgagg atgacattat atttccccgg ctcgaggtca
    29761 gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccc
    29821 agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata
    29881 ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta
    29941 agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc
    30001 agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg
    30061 gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc tgcacgccca
    30121 cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg
    30181 ctgtagcaaa gacacgagta tataagcgaa agcaggatgg gtaaacgtct gaactgtaag
    30241 tgatcgtcag ggctaatctg aaaagtacca gcaaatcaat cggctaaagt gcctcatttt
    30301 atcatcggcc atgtggcctg atctttatct gaaaaataac caaatacaga cagatacgca
    30361 ttacaccacg cgactcgccc cgcaggatca accgcatgtt aaataaaaag agcgtgttgt
    30421 cccgatgaaa caaaaagagc tatggattaa ccagatcaaa gggttatgta tttgtctggt
    30481 ggtgatttat cactcggtca ttacctttta tccgcatctg accacttttc agcatccgtt
    30541 atcggaagtc ctgagcaaat gctggatcta tttcaatctt taccttgcac cctttcgtat
    30601 gccggttttt ttctttatct ctggctattt gattcgccgc tatatcgaca gcgtaccgtg
    30661 gggaaattgt ctcgataaac gcatctggaa catcttctgg gtgctggcgc tttggggcgt
    30721 ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg gcacctgagc gcgatttaag
    30781 caatgcctcc aatgccgctt atgccgattc caccggtgag ttcctgcacg ggatgatcac
    30841 cgccagcacc agcttgtggt atctgtatgc gttaattgtc tatttcgtga tatgtaaaat
    30901 ttttaacccc ctggccctgc cgttatttgt tctgtttgta ctgatgagtg tggctgttaa
    30961 tttcgtaccc acaccgtggt ggggaatgaa cagtgtgatc cgcaatttgc cttattacag
    31021 ccttggcgca tggtttggcg caacattaat gacctgtgtt aaagcggtac cgttgcgccg
    31081 ccatctgctg atggcttctt tgctgaccgt tctggcggtc ggtgcctggt tgtttaatat
    31141 ctcgctgctg ttgtcgctgg tatcgattgt ggtaatcatg aagctgtttt atcagtacga
    31201 gcagcgtttc ggtatgcgtt cctccagcct gctgaatgtg attggctcca acaccattgc
    31261 tatctatacc acccatcgca ttctggtaga aatattcagc ttaagcctga ttccgcaaat
    31321 gaacgcggta cgctggtcgc cgcaagtcga actgacgctc ctgctgattt acccctttgt
    31381 cagtttgttg atttgtactg ttgcgggctt gctggtaaga aaactttcac agcgcgcatt
    31441 cagcgatctg ttgttctccc cgccttctct gcccgctacc gtcagttact cccgctaagc
    31501 catcgcccca gccggggcga ttcaatttgc taaaccatgt cgattacgga taatgaacag
    31561 cattgcttat cagagatatg ccccatatgt tgaggcatat cctaacgaga atctgacaac
    31621 cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct
    31681 gggcgagtta accggcgctg cctgcgcgac gctggtagca gaaattgccg aacgtcacgc
    31741 cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat
    31801 cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaaccc tcccttacga
    31861 cagtttttcg cctcatcagg acattatctc ctcgcgtctt tccacccttt accagttacc
    31921 atcgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc
    31981 gcacagcttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga
    32041 tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg tgatggagca
    32101 cggcgaatac gccacgcgcg gcgcgttgtt ggatctcttc ccgatgggga gtgagctgcc
    32161 ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgatag
    32221 ccagcgcacg ctggaggaag tagaagcgat caatctgttg cccgcgcacg aatttccgac
    32281 cgataaagcg gcaattgaac tgttccgcag ccagtggcgt gataccttcg aagtgaagcg
    32341 cgacccggaa catatttatc agcaagtgag taaaggcaca ttacctgccg ggatcgagta
    32401 ctggcagccg ctattcttca gcgaaccgct gccgccactg ttcagttatt tccctgccaa
    32461 tacgctgttg gtgaatactg gcgatctgga aaacagtgcc gaacgtttcc aggctgacac
    32521 gctcgcgcgt tttgagaatc gcggcatcga cccaatgcgc ccactgttgc caccacaatc
    32581 gctctggctg cgggtggatg agctcttctc agagctgaaa aactggcccc gtgtgcagct
    32641 aaaaactgaa catttaccga caaaagcagc gaatgccaat ttaggtttcc agaaactgcc
    32701 agacctggcc attcaggcac aacaaaaagc gccgctggat gcgctgcgta agttcctcga
    32761 gtctttcgac ggtccggtgg tgttctcggt agaaagtgaa ggccgccgtg aagcgttggg
    32821 tgaactgctc gcgcgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag
    32881 cgaccgtggg cgttatctga tgattggcgc tgcagagcat ggttttgtcg atacggtacg
    32941 taatctggcg ctgatttgcg aaagcgatct gctcggtgaa cgcgttgccc gtcgtcgtca
    33001 ggattctcgc cgcgccatca accccgatac actgatccgt aaccttgcgg agctgcatat
    33061 tggtcagccg gtggtccatc tggagcacgg cgtcggtcgt tatgccggaa tgaccacgct
    33121 cgaagctgga ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact
    33181 gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcgggtggcg cagaagaaaa
    33241 cgcaccactg cataaacttg gtggcgatgc ctggtcacgc gcgcggcaga aagcggcgga
    33301 aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga
    33361 gggcttcgcg tttaaacacg atcgcgagca gtatcagttg ttctgcgaca gcttcccgtt
    33421 tgaaaccacg ccggatcagg cgcaggccat taatgcggtg cttagtgaca tgtgtcagcc
    33481 gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat
    33541 gcgcgccgct ttcatggcgg tagataacca caaacaggtg gcggtgctgg tgcctaccac
    33601 ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg
    33661 tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt
    33721 ggcggaaggg aaaattgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa
    33781 gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa
    33841 agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttaccg caacgccgat
    33901 cccacgtacg ctgaatatgg caatgagcgg aatgcgcgat ctgtcgatta tcgccacgcc
    33961 gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagtc tggtggtccg
    34021 ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct acaatgatgt
    34081 ggaaaacatc cagaaagccg ccgaacggct ggcagaactg gtacctgaag cgcggattgc
    34141 tatcggtcac gggcaaatgc gtgagcgcga actggaacgg gtgatgaatg atttccatca
    34201 tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg acattcctac
    34261 cgccaacacc attatcattg aacgcgcgga tcacttcggt ctggcacagt tgcaccagtt
    34321 gcgcggtcgc gtcgggcgtt cgcaccacca ggcatatgca tggctgctga cgccgcatcc
    34381 aaaagcgatg actaccgatg cgcaaaaacg tcttgaagcg attgcctcgc tggaagatct
    34441 cggtgcaggt tttgcgctgg caacgcacga tctggagatc cgcggcgcgg gtgaactgct
    34501 tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata tggagttgct
    34561 ggaaaacgcc gtcgatgcgc tgaaagccgg acgcgagccg tcgctggaag atctcaccag
    34621 ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcatccctga
    34681 cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg aaaacgaact
    34741 ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac
    34801 cctgctggat attgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga
    34861 aggtaatgag aaaggcggcg tgatcgaatt tgccgagaag aatcacgtta atccggcctg
    34921 gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgtct
    34981 gaagtttatt caggatttga gcgagcggaa aacgcgtatc gaatgggtac gccagtttat
    35041 gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc
    35101 atctgcaata tttacaaatt ctttgcactt ccctgcatta tccgacactg tcaccctcca
    35161 taattcagac ttaccttttt attacaataa ccttatattt attatggact ttggtgatga
    35221 tcaaaacgcg tttttctcgc tggctaacat tttttacgtt cgccgctgcc gtggcgctgg
    35281 cgctaccggc aaaagccaac acctggccgc tgccgtcagc aggcagtcgt ctggttggtg
    35341 aaaacaaatt tcatgttgtg gaaaatgacg gtggttctct ggaagccatc gccaaaaaat
    35401 ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct tacgtaccgc
    35461 gcgcgggtag cgtgttaacg atcccgttgc aaaccctact tccagacgcg ccgcgcgaag
    35521 gcattgtgat caacattgcg gagctgcgtc tctattatta tccgccggga aaaaattcgg
    35581 taaccgtata tcccatcggc attggtcaat taggtggtga cacgctgaca ccgacaatgg
    35641 tgaccaccgt ttctgacaag cgtgcaaacc caacctggac gccaacggca aacattcgcg
    35701 cgcggtataa ggcacaggga attgagttgc ctgcggtagt tccggctgga ccggataacc
    35761 caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg cttcacggta
    35821 cgaacgctga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg
    35881 acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat atcatcaaca
    35941 ccccgattaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc
    36001 tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat agcgcaatgc
    36061 aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg atggatgtcc
    36121 gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact ctgtaaagct
    36181 cggataaaaa aatccccgcg acacggcatg tcgcggggaa aaaaacaagc agtagcaggc
    36241 attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg tattcggacc
    36301 ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt gcgccagctg
    36361 ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga ctttttgttg
    36421 gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa
    36481 aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat gcagatgaat
    36541 gacgtcgcaa gcaattactt aacgatcgat aactaaatga tagatgtgat ctggatcaca
    36601 tacaagatat tttttataac aatcattaat taatttaaaa acctttaaat ttcagttaaa
    36661 tagaaagcga ttatttttat ggtttctgga ctggtgaacg gaggtgatta tttttataat
    36721 aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc aacacaatgt
    36781 gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc
    36841 atgaaattgt tctggcagca cggttatgaa gcgacttctc ttgcggacct cgttgaagcg
    36901 accggagcta aagcgcccac gctatacgcg gaatttacca acaaagaggg gttattccgc
    36961 gccgtactcg accactatat cgatcgtttt gccgccaagc atgaagcaca gctgttttgt
    37021 gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc caactgcttt
    37081 accagcaaag acactccggc tggctgcttc atgatcaaca actgcaccac cctctccccg
    37141 gattcaggag atatcgccaa tacgctgaaa tcacgccatg cgatgcaaga gcgcactttg
    37201 cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg tgacgtgacg
    37261 catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc acgcgaaggt
    37321 gcatcactgg aaaaactgat gcagattgcc cgaacgactt tgcgtttatg gccggaactg
    37381 ctgaaatagg tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcta aattgcagag
    37441 tacagccttg ttatactttg ttattcaaaa tcagctgccc gttgctatct agcgggatct
    37501 gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc ttataggtca
    37561 catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc tgttgcgtag
    37621 tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttacctgca tatccacccc
    37681 ccagcgcccc cacaacagtg gcgacatctt taccgcgacc acctccaaac tgatgcccaa
    37741 tcacgccgcc agcaacagcg ccgagcaccg acccggtaat gcgattttca tcctgcaccg
    37801 gtcgacgatg ggtcactgta acgttgcgac actcctgacg cggtgtttta accgtttcct
    37861 tgattggggt tgcagaaaca acctgagcgt attgcgggcc acgttcaaac acgttcagac
    37921 tggccactgc cgctacgccc agcgcagctg cgacaccaat cccgataccc gccaacattg
    37981 acttattcac gggaacacct ccttctttgt agttatgcca atcatgcaag cgaaggagaa
    38041 aagcagctat cagacagaag atgaaaagcg cgggaatggc tggaaatact cagggattcg
    38101 gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg
    38161 taccatttgt ccagggcgga taaggcgttt atgccccatc cgccagtaca agtcgattaa
    38221 tgcaacttca aacgtggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg
    38281 gttttaaagt aaccatgcag cgcaatctgg tgcattcggt acagcgagat gtatacaaag
    38341 cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctaccg
    38401 actgtggaaa agttcgacag cgataccagc gaaccgtgat ctttatactg ataatttttc
    38461 agcggcttac cgttcatctg cgccagaatg ttgttcattg cgcaggttgc catctgatgt
    38521 gcggcctgag cgcgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg
    38581 ccaatagcgt atatgtctgg atcgcgagtg gtttgcagcg tcggttccac caccagctgg
    38641 ttgatacggt tggtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg
    38701 gctgcccaca ccatcagatc agcctcaata tattcgccat ctttagtgtg caggccgcct
    38761 tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttaactca
    38821 ctatgggccg cagcagagat acgtggcggc aatgcaggca aaatacgttc gcccgcttct
    38881 accagcgtta cgttcagggc ttcgttggtc agacctttat aaccgtaact gtgcagttgc
    38941 ttgaccgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc
    39001 gcaatgttta ctttgccatt cgcgcccagg ttggcggagt atttcaggaa cagattcagc
    39061 atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg
    39121 acacctggtg tattgaaatc gttagaggtg ctacccagcg ccattaccag ggtgtcatag
    39181 gcgattttac gttccggaac cagcagttca cctttctcgt cgcgcagttc tgcaatagtg
    39241 attgttttcg cttcacgatc aatatcaatt acggaaccca gctggaactg gaaaccatga
    39301 ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc
    39361 acttcgtgca gcagcggttt ccacaagtgg ctgtggttac gatcgaccag cgtaattttg
    39421 gcttttttct tgcgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca
    39481 ccgccgccga caatcacaat ttttttcaat ggcgtagtca acgtgaccc
//
LOCUS       DAPQZD010000024        39115 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPQZD010000024 DAPQZD010000000
VERSION     DAPQZD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.024"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            88..645
                     /locus_tag="RO454_001252"
     CDS             88..645
                     /locus_tag="RO454_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDY0933117.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            687..2195
                     /locus_tag="RO454_001253"
     CDS             687..2195
                     /locus_tag="RO454_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDY0933118.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            2246..4243
                     /gene="tkt"
                     /locus_tag="RO454_001254"
     CDS             2246..4243
                     /gene="tkt"
                     /locus_tag="RO454_001254"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDY0933119.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(4273..5118)
                     /locus_tag="RO454_001255"
     CDS             complement(4273..5118)
                     /locus_tag="RO454_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDY0933120.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(5318..6223)
                     /locus_tag="RO454_001256"
     CDS             complement(5318..6223)
                     /locus_tag="RO454_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0933121.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNQQLMTRFVFN"
     gene            complement(6301..7524)
                     /locus_tag="RO454_001257"
     CDS             complement(6301..7524)
                     /locus_tag="RO454_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDY0933122.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(7550..7939)
                     /locus_tag="RO454_001258"
     CDS             complement(7550..7939)
                     /locus_tag="RO454_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HDY0933123.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(7956..8912)
                     /gene="arcC"
                     /locus_tag="RO454_001259"
     CDS             complement(7956..8912)
                     /gene="arcC"
                     /locus_tag="RO454_001259"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDY0933124.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(8905..10329)
                     /locus_tag="RO454_001260"
     CDS             complement(8905..10329)
                     /locus_tag="RO454_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDY0933125.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(10326..11885)
                     /gene="fdrA"
                     /locus_tag="RO454_001261"
     CDS             complement(10326..11885)
                     /gene="fdrA"
                     /locus_tag="RO454_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDY0933126.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(11980..12840)
                     /locus_tag="RO454_001262"
     CDS             complement(11980..12840)
                     /locus_tag="RO454_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDY0933127.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(12846..13514)
                     /locus_tag="RO454_001263"
     CDS             complement(12846..13514)
                     /locus_tag="RO454_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDY0933128.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(13771..14580)
                     /locus_tag="RO454_001264"
     CDS             complement(13771..14580)
                     /locus_tag="RO454_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDY0933129.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            14842..15606
                     /gene="udp"
                     /locus_tag="RO454_001265"
     CDS             14842..15606
                     /gene="udp"
                     /locus_tag="RO454_001265"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDY0933130.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(15641..17131)
                     /locus_tag="RO454_001266"
     CDS             complement(15641..17131)
                     /locus_tag="RO454_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDY0933131.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(17144..17650)
                     /locus_tag="RO454_001267"
     CDS             complement(17144..17650)
                     /locus_tag="RO454_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDY0933132.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(17667..18641)
                     /locus_tag="RO454_001268"
     CDS             complement(17667..18641)
                     /locus_tag="RO454_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDY0933133.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(18666..19295)
                     /locus_tag="RO454_001269"
     CDS             complement(18666..19295)
                     /locus_tag="RO454_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDY0933134.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(19285..20289)
                     /locus_tag="RO454_001270"
     CDS             complement(19285..20289)
                     /locus_tag="RO454_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDY0933135.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(20313..21080)
                     /locus_tag="RO454_001271"
     CDS             complement(20313..21080)
                     /locus_tag="RO454_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDY0933136.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            21297..22724
                     /gene="rmuC"
                     /locus_tag="RO454_001272"
     CDS             21297..22724
                     /gene="rmuC"
                     /locus_tag="RO454_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDY0933137.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            22819..23574
                     /gene="ubiE"
                     /locus_tag="RO454_001273"
     CDS             22819..23574
                     /gene="ubiE"
                     /locus_tag="RO454_001273"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDY0933138.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            23588..24193
                     /gene="ubiJ"
                     /locus_tag="RO454_001274"
     CDS             23588..24193
                     /gene="ubiJ"
                     /locus_tag="RO454_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDY0933139.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            24190..25830
                     /gene="ubiB"
                     /locus_tag="RO454_001275"
     CDS             24190..25830
                     /gene="ubiB"
                     /locus_tag="RO454_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDY0933140.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            25909..26178
                     /gene="tatA"
                     /locus_tag="RO454_001276"
     CDS             25909..26178
                     /gene="tatA"
                     /locus_tag="RO454_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDY0933141.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            26182..26697
                     /gene="tatB"
                     /locus_tag="RO454_001277"
     CDS             26182..26697
                     /gene="tatB"
                     /locus_tag="RO454_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDY0933142.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            26700..27476
                     /gene="tatC"
                     /locus_tag="RO454_001278"
     CDS             26700..27476
                     /gene="tatC"
                     /locus_tag="RO454_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDY0933143.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            27518..28300
                     /gene="tatD"
                     /locus_tag="RO454_001279"
     CDS             27518..28300
                     /gene="tatD"
                     /locus_tag="RO454_001279"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDY0933144.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(28297..28785)
                     /gene="rfaH"
                     /locus_tag="RO454_001280"
     CDS             complement(28297..28785)
                     /gene="rfaH"
                     /locus_tag="RO454_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDY0933145.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            28952..30445
                     /gene="ubiD"
                     /locus_tag="RO454_001281"
     CDS             28952..30445
                     /gene="ubiD"
                     /locus_tag="RO454_001281"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDY0933146.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            30491..31192
                     /gene="fre"
                     /locus_tag="RO454_001282"
     CDS             30491..31192
                     /gene="fre"
                     /locus_tag="RO454_001282"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDY0933147.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(31373..32536)
                     /gene="fadA"
                     /locus_tag="RO454_001283"
     CDS             complement(31373..32536)
                     /gene="fadA"
                     /locus_tag="RO454_001283"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDY0933148.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(32546..34735)
                     /gene="fadB"
                     /locus_tag="RO454_001284"
     CDS             complement(32546..34735)
                     /gene="fadB"
                     /locus_tag="RO454_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDY0933149.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            34925..36256
                     /gene="pepQ"
                     /locus_tag="RO454_001285"
     CDS             34925..36256
                     /gene="pepQ"
                     /locus_tag="RO454_001285"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDY0933150.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            36256..36870
                     /locus_tag="RO454_001286"
     CDS             36256..36870
                     /locus_tag="RO454_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDY0933151.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            36909..38360
                     /gene="trkH"
                     /locus_tag="RO454_001287"
     CDS             36909..38360
                     /gene="trkH"
                     /locus_tag="RO454_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDY0933152.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            38372..38917
                     /gene="hemG"
                     /locus_tag="RO454_001288"
     CDS             38372..38917
                     /gene="hemG"
                     /locus_tag="RO454_001288"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDY0933153.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         9292 a         9848 c        10433 g         9542 t
ORIGIN      
        1 tagcttcaca tttcatactg atgcattgga atgaaataaa atttcatgag attttgtgtt
       61 tgagaaaatt atttcatgag gtgtgaaatg gacgcaaagc tgtacatcac tgagatcatc
      121 aatgaattaa ccttcgttaa ggactctatc cgtgaggatg actgcaccaa attgatcgac
      181 actattcagc aaagccagcg cgttttctgc tatggcctgg gacgcgccgg tttttctatg
      241 aaagccttca ccatgcgcct gatgcatatg ggaaaagagg tttacttcct gacggaaacc
      301 attacgccga actttgggcc tggcgatctg tttatcgttt cttctgcttc cggtgagaca
      361 gcacaactgg tggcgctggc gaaaaaagca cgtcagctcg gcggcgctgt ggcggtgtta
      421 accacgaatc gacacgccac tatcaccgag tttgtcgacg tcatcgtgca aatcaatgcc
      481 ccttccaaaa atcaaaaaga ttctgttttc cgctcggcac agccgatggc gtcgttgtat
      541 gagcaagctt tgctggtgat tgccgatgca ttagtgatga agatggctgc tgaaagtggt
      601 gcgccggaaa gtgaactgtt taagcgccac gccaatctgg aatagtcgtc gccgttattt
      661 gctcgatacc ctacaggaga ataaaaatga gcaaaaaaat aacatccttg tttaacttca
      721 agaagttgca gaaattttct tctgctctga tgttaccaat cgccattctt ccggcggcgg
      781 gtttgatttt agccatcggg atttcattta acattgagct gctggcgaat tgcggcggcc
      841 tgattttcgc caaccttcct ttgctgtttt gtgtcggtat tgtgattggc ctgacaggtg
      901 atggttcttc atcactttca gcgattatca gttacatgat catgaatgcc acgatgggaa
      961 cgcaacttgg cattactgcg gagtccgttg cagccagtaa cggtgattat gcgatgatcc
     1021 tcggggtacc gacgttacaa acgggagtgt ttggcggtct gattgtcgcc atctttgttt
     1081 atctgctcta tacccgcttc cacaatattg aattgcctca gtttcttggt ttcttcggtg
     1141 gaaaacgctt tgtaccgatt attacggctg tggcggcaat tattgtcggc tatttattgc
     1201 cgtggctgtg gataccggta caaaaaggtc tggcgtcgct ttcaacgctg gtgaccagtg
     1261 gacataacgc gaacttcgcg gcctttattt acgccgttgg cgaacgttcg ctgattcctt
     1321 ttggcctgca tcatatctgg aacgtgccgt tttattacaa ctttggcgac tacatgacca
     1381 aatccgggca actggtgacg ggcgatattc cggtgttctt tgcccaattg cgcgacggtg
     1441 ctccgttgac ggcggggctg tttatgacag gtcgtttccc gatcatgatg tttgcgttac
     1501 ctgccgccgc gttggcgatg tatcaggaag caaaaccgga acgtaaggcg ctgattaaag
     1561 gccttttact ctccggtgcg ctgaccacat ttgttaccgg gattacggag ccgctggaat
     1621 atgcattttt gtttgcagcg ccggttctct acatcattca catcttctta tatgccactt
     1681 catttgtgct gatgaacatc ctgaatgtgc acattggtca tccttttgct ggcggtctta
     1741 ttgatttcgt actgaatggc gttatgccaa accgcacgcc gtggtatctg gtgttcctgg
     1801 caggcgcggg ctatgcagcg atttactaca ccgtatttcg ggtactgatc cgcaagttga
     1861 atctgaaaac accggggcgt gaggatgaag agattgccgt tgcaaccaca ttaacgcgcg
     1921 aagaacgtcc ataccaaatc atcgaagcag taggtggttt cgacaatatc gaagatgtgg
     1981 atgcctgcgc aacacgccta aggctggcgc tggtagacga taagaaagtg aatgaaaaac
     2041 ggctgaagga gttgggggct gcgggtttag tgaaactggg tgatggcggt gtacaggtga
     2101 tatttggcgg caaatcgcaa atcctgcgcg acgaaatcaa aaccgtaatg agcagaccgc
     2161 gcccgacaga aatgacagtg gcctgtgcta actaattcga gattagcggg ctggtttcag
     2221 cccgccagac agagagagct gaagtatgaa ttcacaatta ctggctaatg ccatccgaat
     2281 gttaagtgtt gatgcgatcc agaaagcgaa ctccggtcat ccgggcgcgc cgatggggat
     2341 ggcggatatt gccgaagtgg tctggcgtcg tcacctgcgt cataacccaa aaaatccgca
     2401 gtggtttaac cgcgaccgct atgttcagtc gaacggtcac ggctccatgt tgatttacgc
     2461 tttgctgcat cttacaggct acgacctgtc gatggatgat attcgcgact ttcgtcagct
     2521 ccactcccgc acgccgggtc atccagaata tggttatacg ccgggtgttg aaaccaccac
     2581 cggcccgttg gggcagggcg tagctaatgc ggtggggatg gcgattgcgg agaaggcgct
     2641 ggcggcagaa tttaacaaac cgggctttaa tattgttgat catcacacat ggttattttt
     2701 gggtgatggc tgtctgatgg aagggatctc ccacgaggcc tgtggtcttg caggaacgct
     2761 gaagctcggc aatctgattg ctatctggga cgataacggt atttcgatcg atggtcacgt
     2821 agaaggctgg ttcgctgaag atacggctgc ccgttttcgt gcctacggct ggcatgtcat
     2881 tgaaggcgtt gatggtcacg atccagaagc ggtagatgct gcggtgcgtg aagcgaaaag
     2941 cgtcacggat aagcccagtc tgctgtgctg caaaacgatt atcggttttg gttcgccaaa
     3001 taaagccaac agccacgatt gccacggctc ggcgctgggt gcagatgaag tggcgctggt
     3061 gcgtgaacgt ctgcaatggc cgtatgcccc tttcgaaatt cctggtgaaa tttatgccga
     3121 atgggatgcg accgagaaag gcgcgcaggt tcaacaggaa tgggatgcac tgtttgccga
     3181 ctacgcgaag cagtggccgg aactggcggc ggagtttact cgtcgcatga agggtgattt
     3241 gcctgctggc tgggtcgaga atatgcagaa atatgtgcac gatctgcaat cccatccggc
     3301 ggcgctggca acccgtcagg ttagccagaa atgcctcaat cacttcgcag atatgctgcc
     3361 ggaactgatg ggcggctcgg cggacttgtc gccctctaac ctgactcgcc atcagaaatc
     3421 ggttgatttc accggagaaa atccggcggg gaattacatc tcctatggcg tgcgtgaatt
     3481 tggtatgtcg gccatcatga acggtcttgc gctgcatggt gggtttatcc cttacggcgg
     3541 tactttcctg atgtttatgg aatatgcgcg taatgcactg cgcatggcgg cgctgatgaa
     3601 gatccgctcg gtgtttgttt atacccacga taccatcggc ctgggcgaag acggcccgac
     3661 acatcagccc gttgagcaac tggcgtcgct gcgtctgacg ccgaatatgg aaacctggcg
     3721 cggttgcgat caggtggaag tggcggtggc ctggcaacag gcgattgagc gcaaagatgg
     3781 cccaacgtcg ttggtattaa cgcgccaacc tctggcgcaa cagccgcgca ccgctgcaca
     3841 gttggcagag attgcgcgtg gtggttatgt actgagtgat tgtgacggtc agcctgagat
     3901 gatcctcatt tctgcgggtt cagagattga gttagtggtc agcgccgcaa aagcgttaac
     3961 ggaagaaggg cgtaaagttc gcgtggtttc catgccgtgt accgagcgtt tcgataatca
     4021 ggatgcggcg tacaaagagt cggtattgcc aaaagctgta cgcaaacgtc tggcggtaga
     4081 agccagcatc gccggattct gggaacgcta cgtgggcctg gacggtaaag tgatcggcat
     4141 gacgagcttt ggtgagtctg ctccggcgaa tgtgttgttc aaacactttg gttttacgcc
     4201 ggaaaatgtc ctggcgcagg cgcgtgagct actgaacagc taaacctgca caaggggtga
     4261 aaggatgacc ttttacccct tgaaacggga gccaagaaac tcttcacggg tgcggtattt
     4321 ctcgtaatgc gccttgtttt tatacataaa caacagcacc agcatatcaa taacgcccat
     4381 tgccggaacg cgagtggagt cgaaggcttc aaagaagatt ttatcgctga ccgcggtatt
     4441 gagttgatgg gtggagagtt ttgataacgg agatttaata tagttagtga tacaaacgga
     4501 tttggcaccg cgctctttca ccactttaaa gatatcgacc attaagttgg tgctgccaga
     4561 gttagaaata ccaatcgcca ggtcgcgttt ccccatagaa tatgccgcat taatttgcat
     4621 ttccgggctg acgtgaacaa cggtttttaa tccgagttgg ctgaatttat aagcactata
     4681 aacggccagt gggtttgaat ttccggcagc aaaaaagaaa atagtatcgc aattagcaag
     4741 tagtttgaga atggactcaa gcacgtcggc attaaaaaat tgcgttgatt gtattaaatc
     4801 ttcggcctgt ttttttaata aaaaggttgc caaagatgtt atatcgctga agtcggtatt
     4861 ttcaggatag ctattgtcag gattaatgac ttcttttgct aatgcaattt ttaaatggta
     4921 ataaccctga taacctaatt tcttacaggt gcgtacgata gtcgcttcac tggcatggct
     4981 tttttctgat agttcagtga cactatagtt cacgacttca tgtgcgtggc taagaatata
     5041 gtcagcgacc tttttttccg cctgatgcag tgtagggtaa ttgctttgaa tcagactttt
     5101 tacggttatg ttattcataa ttagtaaatt tcattctgac agaaagtgct gattataaca
     5161 tagttttcag gcgtggatag gtgtgaaaaa atgaaggtgt gcagtgaacg taatgattaa
     5221 cagaccggtt aattttcatg aattacgtca actgcacaaa aataatttaa acgggcgctc
     5281 taattaaagc aagacttata tttttatcat tgtattgtca attaaataca aatcgtgtca
     5341 ttaattgctg gtttcttaaa tattctatgc accactgaca ggctttgccc ataccatcgg
     5401 aacgagcggc aagaaataac ttggtggcgt gtttatgttc gcgcattggc tttttaagca
     5461 atttaccgct gttcaataac ggtaatgcca gatgatgtgg aatataacca ataccgacgt
     5521 tttgttctat taaggcgatt gccgtagcga agtcaggaac aaaaatgggt ttttgtcctt
     5581 ccaacaacca ggcttgcatc ggggggaagt taacggaggt atcgcggata caaaccgctg
     5641 gataatgacg caattcactg ttttctaatg gatgccgctt cgctgccagt gggtggagtg
     5701 ggctgacgac aaaatcccat tccatttgtc cgaccggttc gctaatgatg ccttcactgc
     5761 ttggtactgc atgaggagca ccgtagacca gatcagcacg cttactgtac agcgcatccc
     5821 agcagccgtt gtatacctca agatctaccg tcagtgttgt tgatgtgaat tgccgttcaa
     5881 aatctcgaat aaatgccacc agcaaatcac caggaataat attgtttacc gcaattgtca
     5941 gttcacgctc aataccatcg tgaatcagca ctgtgttgcg ctttaatgct tccaggtcgc
     6001 caagaatagt tttgctatgt tcgatgaagt aacttcccgc tggcgataat tcaatatagc
     6061 gtcccttacg aatgaacagt tcaacgccca ggctctcttc cgctttcttg atggtgtaac
     6121 tgatggcgga agggacttta tttaactgct cggctgcggt ggtaatgctc tggtgtctgg
     6181 cgaccagatg aataacacga agcgtttcgt ctgagatgaa cataaggctc cctggtcagt
     6241 ggtaaagtgg ctgaatctct ccagattcag ccgtaatcat tgcctgagta tacccaggaa
     6301 ttagcggtgt tgacttgccc gactgagaat cgcatacgcg atacatgcca ccagaatcga
     6361 gtctaccgaa gggatcgtcg tcagggtgaa taagcctttt acggtcataa agccaaccgc
     6421 tgcgcctatg atccatgctg cgaatgtcag cactttaatc tgtgactcct gggctaacac
     6481 tgactcttga tagccattac ggcgataaag gaaaaagtca gcgatataaa ttccggcaac
     6541 gggtggcgtg gcgataccca ggaacagcag aaacggaata aaccacgcca taatatccat
     6601 actgcctacg atggcggaaa ggatacccag cgccacggta atctgccatt tgggaaagcg
     6661 ggtaagcagt gtggaaacca ccagcgtgcc ctggaacata ttgcccgcgt taccggtgac
     6721 gcaggcgaaa atcaacaaca gcgccgcagg cagaactgca ccgaataccg ccatcgcacc
     6781 cagtaaagat ccctgaccac taatggcgct cggcgttgca cccgcccagt agagcagagg
     6841 gtaggcaacc agaaacgtca cgcctgcggc aatcagcgca tgtttgcggt tatgcacaaa
     6901 actaccgaaa tcaggcagtg tggcaaccag cacaataatg gtgccaacaa cggtggaaac
     6961 ggcgacacct gtattcatct ccacaatcgc cgctggtact gccacttcgg tatgggttgc
     7021 cacatagagg atgtaacaca gcaccagcgc gataaccggc acggcaacct gggcaatttt
     7081 tcccagaacg gcaaagccaa atgccgtaga ggcgacaaaa atgacacagc cagccgccac
     7141 taacagtgcc agtggaactt ccagtccatg ttgagccagt aagtcatgta ccgaatgacc
     7201 aaacatattg gcggtaacgg caatccagcc gaacagactt atcgccatca aaatgttaat
     7261 ggcgatggcc cctttttcgc caaacgaata tttcacaatg ccatacgttg gcaggcgggc
     7321 tttttctccc acgctaatgg tgatggctga aagtaaggtc agaatgacgc cgcctaacaa
     7381 tacgacgcta attaaactgc cgctggaaag ttgattgccg aggcttgagg atgacaacag
     7441 cacaggtgtg acggcaatac ccagcaaaat catggcaata cgcaatcctg atgccgtatt
     7501 cagattctct tcttttttac gcatagtatg ttccctttat aaatgcgcgt tacacagtat
     7561 gaaaatgccc ggtgtattta cgccagctac ccagttctgt aatttcccgt tggccttcgg
     7621 tcagccagtt ttctgccagt acgcccagca cttcgctacc atcggcaagt ttgattttgc
     7681 caattgccag cccagcgggt tcgctcatta acagagcggc aaaactggcc agcggtaatt
     7741 gccaaatttc tacgtcgacg tgtgttcctc cttcattcac gcgaatcatt cccggatggc
     7801 gatcattaat actccacaac cggtaatggg cgtcagttcg gtcttcacgg acaaaaatac
     7861 cgcccgcttt ttgcatattt gggttgagtt ccaggccgcg catcagcgtt ccgttaacgg
     7921 ctaaaagcgt tgtagtcatt gtgttctcca gagaattaaa gggttatcca tgtaccgctc
     7981 tgatgcgcca gggctgcttt tatggtttgc ggtgaagtaa tcaggccgct ggcctgtttg
     8041 ccttgttgct ggctggcatt gatgaaatca acaatggctt ccactttggg ttgcatactg
     8101 ccgacaccaa actgaccttc ccgcaacagc gtttgcgctt cggcaacgct gatagcgtcg
     8161 agccactgtt gttgtggtgt accaaagtta atcgccactt tttctacacc ggtggggatc
     8221 accagcagat ctgcgcctaa ctgactggcg agcagcgcgg aggccagatc tttatcgatc
     8281 acggcttcta cacctttcag ttgttgcccg tctcgcacca ccggaatacc gccgccgccg
     8341 caggcaataa ccagatatcc ctggcgcacc aggtgagcga tggtgtcgtg ctcaataatt
     8401 tccagtggtg caggagaggg aactgtacgc cgccaaccac gcccggcgtc ctccatcagc
     8461 gtccagccga gttgttgttg gcgttgttgg gccgttgctt catcgagaaa cgcgccaatc
     8521 ggtttactgg ggctggcgaa agcatcgtca tgtgggctga ctcgcgtttg tgtcaccagg
     8581 gcaattaccg gtttgtttat gccacggcga gcgagttcgt tatgcagcgc tttctggaac
     8641 atgtagccaa ttgcaccttg tgtatcaccc acggcgtaat caagtggaac cggagaaact
     8701 tcgttactgg cgagttcaga gcggcgcaga ataaagccca cctgcgggcc attaccgtgg
     8761 gttagcacca ggtcccatcc ggcttcaacc atatcaacga tatgctgcac gctttccatc
     8821 actgcaacat attgatcggg aatactattg cgttgctcgt cctgaatgag cgcattgccg
     8881 ccaaccgcga cgacagccag tggcttaacc atgatggttc tcctttgcga cgatctccgc
     8941 cagtgcacgt acagcggcga caaaacaggc catcggcgca gtggtgatcc cggcaccaat
     9001 ttgcccgacc cccgcttgtt tgtgggcgat acccgtattg atgaccggta aaatgccgcg
     9061 atcggtcact ttacgcgcat caatccctgc tgcggtgggg gcaaaattga gggctggaag
     9121 tgtaaatgcg gggttaccgc cgagggtgat ggcttgcatc cgacggctgt tattggtggc
     9181 atcggcaggg gtgcctccca caaatttgac gatcgccgga gacgacgcca tcgcgaagcc
     9241 acccacacct gcggtttcag taattgcgct atcacccagg tcagcagcag cgtcgtcaac
     9301 gccaaatccg gggaagaaca gaccttcaac ggcattggca ggagcctgga accagcgatc
     9361 gccagtaccg gaaatccgaa taccaaaatt caccccgtta cgcgccatca ccgtaaccat
     9421 tgaactgaac ggtacattgg cggcggcatc catcattgct ttacaggcgg ccatcgacag
     9481 attgaggaag aagtgatcgt taccggtgat aaatgccacg acgcgctgga tatgttcctg
     9541 tggaagagag caggccagca atgccgggag caggcgctta atcagcaaac cggtagcggc
     9601 agcattgcgg ttatgaactt catcccccat atgcagcgcc tgcgccatta atggcttgag
     9661 ttccagctcg ccgtgttgtg cgattgctgc tttcattgcc ggcgtcagtt catcgcgcat
     9721 ccaggccaga cgattgagaa cttcatcgtt attggcgcca aaacggagaa ccttacccag
     9781 accttcattg aaattgctaa aagtacggtg gccgttggtt ttgttttcta ccacccataa
     9841 cggcatcgac gggctgatga tgcctgccat tgggccaacc gcgtgataat gatgacaagg
     9901 ggcaaggtcg atttcgcctg cgtttatctg gttttcagcg tcttgctggc tggtggccca
     9961 accttcgtac agcatggcac caataatcgc cccttttacc ggaccacaca tctcttccca
    10021 ggcaatcggc gggccagagt gcaaaatacg tttttgttca gccattgctg gaatggcctc
    10081 tttagcaagc atgacatcca ccagcacggg ctgtgcttcg agataacgtg ccatggcgat
    10141 ttggttggca ttttctacca ggggatgacg tagcaacgat gccagatcca atccagcgtc
    10201 aatatcgcct tgtgctggtg gctgccagtt gagtgcaatt gcgcttcccc cggcatgttg
    10261 aatattgtca gcaaaactgt gtagtcccgc gttcacaacc ttcaaaggct gattaaataa
    10321 ggtattcatg cgtttagccc ctttttctgc aactgagtct gtgcaacggt gctggcccat
    10381 agcgccgcct gtgcattgct gctggcgatg atgacccctg cattttgtag tgcgcttatc
    10441 tgctgactgc gtatttgcgg atcagcttcc gttccgcaaa cgtgcgcaat cagcaacgga
    10501 gcgttattca tgtcgatgtg tgaaagttgg tccagtaatt cgctggcagg cgtcgtggaa
    10561 gcgccataac caagtaccag gtcgaataac acgacggcag tgtgtgagtc gttgagttcg
    10621 ttgagcagac gttgattgcg cagagtcggg tcgatcattg gatgcggttt gccacgggtg
    10681 aaatcatcgt cgcccatatc gatcaacgtg tgatcttcgc tttgccagat atttgccaga
    10741 gcacgattac ccgcaactgg cgtattggaa gctgcgctga atcccttttg ttggcagata
    10801 agttgcgctt cgtagcagaa ggttcctccg gcgaaaacac cgcgtatcgc ctggcgatgg
    10861 gctggcaggc gctggcaggc gttttgtaac attgtgagat cgtcgcaggg tacatcggtt
    10921 tcgacagccg gaagtggctg agcattcagt aacgccacag cgatgtcggc ggcgctggca
    10981 agtgtggtgg cggcagtgat attgggacgt gccagatcat gcggattcgc cccgaggaaa
    11041 ttcaccacca cgggttttcc acaagcctcg gcgcgctcca gaatagtccg ggcaacgatc
    11101 ggtgaaggcg gttttgaaat cagcacaatt acgcgagttt catcgtcttg cgccagcgca
    11161 tctaaagcga agagcatcga tattccacca atctctttac tgagatcatg tccgcctgtt
    11221 cccaacgcct gagaaatacc cgctccgagc tggtcgattc ggcaagtcac ttcctgtagc
    11281 ccggtgcctg acgcgccaat aacaccaatt gctccgcgtt tcaccacatt agcgaagcct
    11341 aacggaatgc cgttaacaat ggcggttcca cagtcaggtc ccattacgat gcgctgacgt
    11401 tctcgggcaa gcgttttgat ggatttttcc tgcacgatac tgacgttatc gctgaacatc
    11461 atcacgttca ttccgaggtt taacgcttta attgcttcgg cggcggcgta gtctcccggt
    11521 acagaaatga gtgccaggtt ggcttcgggt tcggcttcca gtgccatttc caggctgacc
    11581 ttttccggtg ttttaatgcc actatccgtt tcatcatcgg gtttgctggt cagacgttgc
    11641 tgggccagta tcagtgcttc attgcaaata tcttcttcac ccatgactgc aatcactaaa
    11701 tcattgggac tggcgttgat ttcgttaccc agacccgcat cgcgcaattg ttcgagatta
    11761 ttgggcgttc ccatgacgac cgaggcctgt tgaataccgg gcaacttgct gatttgcgcg
    11821 gaaatctgca tcagagaaac tgagtcctga taaagattac tgaaaacttt atatttaact
    11881 ttcatgctgt tacgcctgtt tcattccgcc cgatagcaaa attcgggcaa agcatccccg
    11941 tgtagcccca taaacgggct acgggagaaa aggttgaaat caattcatgg tgttcagggt
    12001 tcgaaagcca tgcagcattc ctgcaaggca atctactcct gacgttgcgc caaattgcat
    12061 gacctgttct gcacaggatg cgattgtcat tgccgatgcg ctcccaacca gttgtgcgag
    12121 taactggcaa atcggttctg aaaaatgtcc ctgaagcgca cgctccagat aatgacgact
    12181 gatatccgtg gtgttgtgcg catgttgatc aattgcctgc tgcaaccggt attgatgatc
    12241 ggcaagtggt gcagggagtt gccataacca gagcgctgcc agatagccca acaaatagtc
    12301 atctccatcg ggcgttaagc ctttaccaaa gccgatcagt tgtggcacct ggcgttccag
    12361 cgcatctgga tgctccataa tcgctaatcc ctggtgcgtg agactatccg gcaggtgcaa
    12421 tgtgctttga acgttatgct ccagacaaaa taaaagccgt tgcgatgcca tcgttgggta
    12481 gtgctggaaa accaaaggga atgcctcaag tgtcagcatt ccccccacaa atggcgactg
    12541 ccagagcgtt gcgttatcga gcgccacaca aaatcgaggt gtccgcagca agcctggggt
    12601 taactgaatg ggatctgaat gggcaatctc tcttcgccag tcccaacatt caggtagcca
    12661 gactctggca gcatcaggga gattcagata cgtttgcgca cacaacagag tgatgaatgt
    12721 tcccgcgtct gaatacagat ttaccgcatg tttaaaagcg ctatgtagac gtaaatcttg
    12781 cgtttcatcc tgcgggaaaa gatatcccaa cgacgatgcg caaagcgtaa cctgtaccat
    12841 cggcgttagc cttccagacc tgcaataatg gctttggaat cagtaaccca accgaaaatg
    12901 gcaccttgtg ctttgatcat ctcgagggcg tatttctgga attccgggaa ataggagccg
    12961 acacaatctt ccgggataat gcattcatag ccacgatcat ttgcttcgcg cacggtggtg
    13021 gttacgcaaa cttcggttgt tacaccacaa actatcagcg tcttgatacc gtggttttgc
    13081 aaaatcagat gtaagtcggt ttgataaaac gcgcctttgc caggcttatc aatcaccggt
    13141 tcgcctgcca ccgggtacag ttcaggaatg atgtcatgac ctgcttcacc tctgaccaga
    13201 atgcgtccca tcggtcctgg ttcaccaata aaggtttttc cgccgcgcgt cagtttggct
    13261 ggcgggcagt cgcttaagtc tgcacgatgg ccttcacgag tatgaataac cataattcct
    13321 ttttgacgtg cggctgccag cacctctgta catggtgcga tagccgtgcg tacccgtgaa
    13381 acatcattgc ccagtgcctc accgaagccc ccggcttcga caaagtcacg ctgcatgtcg
    13441 atcatgacca gtgcagttgt gcgcggatca aaaggtaatt caaagggttg agcctgaaaa
    13501 atggattgcg tcatggtaaa tctcctgtct gatgtttaaa cgatccagtt gttctgagaa
    13561 cgagtattca ggagaaaagc ggcacaaaac aggaaaaaag tttgccgcac agcaacgaaa
    13621 tttttgcatt cagcaggtgg tgtgatgacg aatgtgaagg gagtcaaaga gtggcataca
    13681 gcaacaaatt ttttgatgct gtatggtttt gagattaaaa aaatcgttga aattgtgtga
    13741 gcttaatcat cagatttagc gactatggcg ttacttttta acaccatact gtgtaaacca
    13801 ttccaacatt cgctgccagc cgtctttcgc agactcggca tgatagctcg gacgataatc
    13861 ggcgttgaac gcatgctctg catccgggta cacgataatc tctgctttcg cgtttgctgc
    13921 ccgcagcgcc tggcgcatcg tttccacact ctcttgcgga atgctgttat cctggccgcc
    13981 atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt gtttcggtga
    14041 attcagcgac ttatcgcccg tcagtttgcc gtaccacgcc actgcggctt ttagctgtgg
    14101 attatgcgcg gcatacagcc aggtgatacg tccaccccag cagaatccgg tgatcattaa
    14161 acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga gatcggccag
    14221 cacctgcgag tcaggcactt ttgctaccag accgccaagc aacgtgggga tatcggcaaa
    14281 atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat acccctccag
    14341 cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt cctgcactac
    14401 aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag caggcatgtt
    14461 atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtccg gagtctgaac
    14521 gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca gattgttgtg ttgttgccat
    14581 ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca gcatgcaaca
    14641 aatcacattg cctgaatcgg ctcatctttt atgcagtcct gcagaatgaa gagtgattta
    14701 tgtgatttgc atcacttttg atgggtaaat ttatgcaaca cattttcgtc atggtgatga
    14761 gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt ctgactaaac
    14821 cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg cctcactaaa
    14881 aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg tgtggaaaag
    14941 atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt caccacctgg
    15001 cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg cggcccgtct
    15061 acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct gcgtatcggt
    15121 acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac cacggcgtct
    15181 gttcgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc tgtcgctgat
    15241 ttcgaatgta cgactgcgct ggttgaagct gcgaaatcca ttggcgcgac gactcacgtt
    15301 ggcgtgacag cttcttctga taccttctac ccaggtcagg aacgttacga tacttactct
    15361 ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat gggcgtaatg
    15421 aactatgaaa tggaatctgc aaccctgctg accatgtgtg caagtcaggg cctgcgtgcc
    15481 ggtatggtag cgggggttat cgttaaccgc acccagcaag agatcccgaa tgctgagacg
    15541 atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg
    15601 aaataaaaaa acgctcccca acttcgggga gcgatatcag ttattgacgc cgtaaacgca
    15661 ttattttgcg gattattggc gtcagtagcg taaaggctgc cagagtcagc aatgccagag
    15721 tgatcggtcg ggtatacaga aagtcgagtg acccaccaga aagcgacagc gcacggcgaa
    15781 aattactttc cgccattggc cccataatca atgccagcac tacgggtgat gccggatatc
    15841 ctcctttctg catgaaatag cccaccacgc ctgcaatcag cataatgcca acatcaaaaa
    15901 ggctattgtt caacgcataa gtcccgacca cacaaagtgc gaggatcatc ggtgtgagaa
    15961 tattgccggg aattttcacc acattaccga tgactttcaa agagagcagt ccaagaacta
    16021 gtagcatgaa gtaacagaag atcatgccga caaacagtgt ataaaccata tcgccgtggt
    16081 cggtaaacat cagcgggcct ggttgcattc cctgtaacgt taacgcgccc agcatgatgg
    16141 cggttaccgc atcgccaggg atccccagcg tcagcatagg taacaatgcg ccaccggtgc
    16201 agccattcgc gcctgcctca caggaggcca cagctttaat ttcaccctta ccaaagtttt
    16261 ctggtgtctt actgaaacga cgagtttcgt tataggcaac aaaagctgca atatccgccc
    16321 ccgcaccggg gatcatgccg ataaaaaccc ctaatcctga tgagcgcaaa atggttaatg
    16381 cgatgcggcg tagtgtttgc cagggcagta acagactgcc gatagccacc ttcgccccct
    16441 gacgaatttg ctggttttgc tccatagagc ggaatgcttc tgaagcagca aataaaccaa
    16501 tcatcaccgg gataaatggc acattcatca gctccacaaa tccacctgta aaccgtggaa
    16561 aaccgcccat tggatcaagg cctatcgttg cgatcagcaa tccgccaacc ccggcaatca
    16621 gccccttaat cagcgactca ccggaaatac tggcaataat gctcagtccg aaggttgcca
    16681 gggcgaagga ctccgaggca ctaaatttca gcgcccaact ggcaagtacc ggagagagaa
    16741 acatcagcac gataatgctg agcgttccgc cactaaagga agagaacgtg gcggcactca
    16801 atgccagccc tgcttttccc tgcttcgtca gctcatatcc gtcaatggcg gtggctgcgg
    16861 cggcaggcgt accgggaatt ttgagcaaaa ttgcggtgat agaaccgcca tagacgccgc
    16921 caaaaaagac gccgcaaatc atcaacaagc cagaaacagg ctccatgccg taggtgaaag
    16981 gcagcaaaat cgccacaccc atggtcgctg tcagaccagg cagagcgcca atcacaatac
    17041 ccagcatcac cccaaaagtg gcaaacagca gcgcctgagg attattgagt aatgtcgaaa
    17101 aaccctgagt taaaagttca ctttcaaaca ttgctggccc ctgttattcg aaaaatgtgc
    17161 cgacaggcaa tggcacctgt aacgcttcga caaacagcaa ccagaggatt gccgttaaca
    17221 gcagcgcaat acccggtgtg agtacccatc gcctgtagcc catcaacgtc ataatgacga
    17281 tgaggaaaac aggagtagta atgagataac cgcagtagct catcgccaca atataaaacg
    17341 cggttgccgc aattcctgtt gccgttttcc tgaatgccgg tttttcctgt gcgttggcgc
    17401 aagaggctgg ttttgcctgc aaaagatcgc gtatcaccag caagacagcg agcacaatca
    17461 gggcacaggc atagaaggta gggaaggcac ttgcaccggg atccgtttca atggcagttg
    17521 ctggaaaatc agcggcatta atcatcaccg cgccagcaac cacacaaagt aacagcgcca
    17581 ccaccatcgt tgatgaaagg cgcttgcggt gcggtacggc gagttgagca acagactgtt
    17641 ttgtgttcat caaccgtacc tcaggtttat tttttcaggc ccaggcgagt cagcaactcg
    17701 tcaaagtact tttcctgttc gctgatttgg gtctggaagc tggcagcgtc aagccatgca
    17761 tagttgagat tcaacttttg tagtgcatcc tggaatgcag gctcttcagc cgtctctttt
    17821 gctgcctttg ccagaacatc caccacatcc tgcggcgttt tttgcgacac aatcaggccg
    17881 cgccaggtgc cgatggagag atcaacgcct ttctctttta acgtcgggac gtcaggcatg
    17941 gttttcattc gctcatccgc catcactacc agtgtcttca gcttgccgcc tttcacatgg
    18001 ttgataactt ctcctgggct tacggaaacc gcttcaatgt gcccgcctaa caggcctgta
    18061 atggcagggg ctgcgccgtc atacgggaca tgagaaaact ttgcgcccgt tttgtcttcc
    18121 agtgcagctg ccgccagatg ccagatagct ccggtgcctg agttaccaat gcgtactttt
    18181 ccgggattcg ctttggcgta agtcataaat tcttcatagc tattccacgg cgcatcggca
    18241 cggactgtga tagcagccgg atccgcattc agacgggcaa tgggtttaaa gtcgctggtt
    18301 ttaaaacgca ccattccgag gctgggaagg gtggtcagtt caaccgtgcc taaaccaatt
    18361 ttgtaaccgc cagggcgggc agcggcgatt tcactcaggc cgatagcacc gcctccgcca
    18421 ggtttattga taaccccgat gctgacgggt aaatggtttt tggcggcatc agcaaaggca
    18481 cgggcaacca gatccgtacc gcctccggca gcgtagggaa cgactaactc gatctgtttg
    18541 gttggccagg aggcagcctg tacaggcgca atgctgaggg cagcgcctgc aagaagtacc
    18601 attgcagcca tttttttcgg taaggtaaga tgttttatca ttgcttttat atccttattt
    18661 atcagttagt tgctatttaa attgttccat gcgttcataa acagtactgc attttcatag
    18721 acttgctctg gcgtcatgcc ggggtgatat agcgcagaac cgaggccaaa gccgtttgcg
    18781 ccgtggctca gataacgcgc catggtttcc ggtgtgatac cgccaaccgg taacatcgga
    18841 acggattgtg gaataaccgc acgccaggct ttggtaactt ctggcgtaat ttgttcagcc
    18901 gggaacagct tgatggcatt agcaccatgt tcaatggcag taaaggcttc ggtgggcgtg
    18961 gcgacgccag gcgcacaata catacccagc gcacgggcgc gacggatcac cgcagtatcg
    19021 caatgcggca tcacgataat ctggccccca gcctctttaa ccgcaaccac ctgctctacc
    19081 gaaagcacag ttccggctcc gaccaggcag tccttaggca gggcgtcgcg cagtaaactg
    19141 atactttgca acgcctccgg cgagttcata ggaatctcaa tcaggcggaa tccggcggcg
    19201 tacagttttt caccgatggc gacaatctca tccggtttca cgccgcgtaa aatggcgact
    19261 aacccggttt tttgcaaaag cgtattaaat ggcatgggta cattccctgg cgttggtgga
    19321 cagtaacccg gcagcgtggg cgattcgcca taatccctgt tcagtggcat taggaatggc
    19381 gtgtatctgt ctgtcgggaa aaacgtggct gaatgcttgt gaatagcggc tgttcagatt
    19441 tgcgctaccg attaaggcga tcggtgttgt ggcgggtaaa tcacgtagca aactggatgc
    19501 cagaccacat aattcatggc ctatcagtaa tccggaaagg taatccccct gcgaggacga
    19561 cgtaagatta ctggtcagca ggcgcgaacg ggtcgaaaac agcgtcgata ataatccggt
    19621 ctgatgatgc tcttgtgctg ctgtcagccc gtgggtgaag gcatcccagt gcagttcatc
    19681 tccaggatgc atggtggcac cgagaatcga atggcgggat aaaacatcaa ataactcgcc
    19741 cgtcataaag gttcggaaat cggtaatggt gttgttttca actactgccc atttcgcatg
    19801 tgtgccgggt aagcctatca gcactggcat accctgatgg atcgcatttt gcagggacgg
    19861 ttccatcgag atagcaccga agatttgtgt ctcttcgcca cgcatcactt cgggcatttc
    19921 accttcttta atcacgccag gaatgatgtg cagcattgtc tgctgctggt ggataacagg
    19981 cgtcaactgc ttcgccagac cctcgagagt gaccgggcaa ggtaaataag ctgctggttg
    20041 ccacccttgc gcactgccga gcatgccgca agccagtacc gggcatgggg tttgtgccag
    20101 ccagtcgccg cagacacgta aaaacgtatc gtggaaagcg tcctcacgtg ttgctgcctg
    20161 ggagggtaat ttcatgatcc cccaggggaa ttcacgtttt tcttgtggat ttggtgattc
    20221 gccaaaacgc catgcccgaa gcgatgacgt tccccagtcc agggcaataa atgagggtgc
    20281 tcccggcatt agtcgtttcc tttaatcatg gtttatttat ctttgcggac gtgcttaccc
    20341 cagcccagtt cagcggatat ctcttctgca caggatttga cgtaaggtgc cagttcttcc
    20401 agacgcgcca gcgacatata cgtggtggta ctggcgacag aaatagccgc cacaatttca
    20461 tcccgggcgt tgtaaaccgg cgctgcaacg caacggatag tcggctcgtt ctcttccaga
    20521 tcaaaagcaa aaccactggc ggcataacga cgcatgttct gaataaatgc gcccagtttt
    20581 gtttcatcac cacaggtctt aaacagcgtg cgccactctt cttctgttcg gttgaggatc
    20641 aatgccttgc cgatgccggt gattgccagt ggcatgcgat ggcctgggcg tgagcgcatt
    20701 tccagcccgc gctgactgtt gatcttctcc agatacagca cttcgtcgcc ttcaacgatc
    20761 cccagatgca cggtgtccag ggtgtaacgg gcaaggcgtt gcaaaacggg ctgggcaatt
    20821 tcgtacagcg aggtgctttc cagtgctctg gcaccgaatt cgatcagttt ggcacccagt
    20881 tggtagccct gcgttgggac atggcgcagg tagcgttgtt ccaccagaac ggtgactaaa
    20941 cgatgggttg tgctccgggt cgtgccggtg aactcgccga tactttttag gtcatggcag
    21001 ccgttataaa cagcattaag aaccgccagt ccacgtaata acgtttgtgc gcctgcggtg
    21061 gttgaagagg tttctgccat acgtttctcc tgatgatatt gctgaagtgt tccacataat
    21121 gacgtgggga aaaggtgata tatcccacaa tataaacttc atctcatatt atgggataaa
    21181 ataatgaaaa ttaacgatcg ttttgcgtag cgcctcgcag gaaacttctt tcaaactgga
    21241 cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg
    21301 atttctcaat catggtttac gcagtcattg cgttggtggg tgtggcaatt ggctggctgt
    21361 ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg
    21421 tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgcgcagagt
    21481 gtgaattgct caataacgaa gtgcgcagcc tgcaaagtat taacacctcg ctggaggccg
    21541 atctgcgtga agtgaccacg cggatggaag ccgcacagca acacgctgac gataaaattc
    21601 gccagatgat caacagcgaa cagcgcctca gtgagcaatt tgaaaacctt gccaaccgta
    21661 tttttgaaca cagtaatcgt cgggttgatg agcaaaatcg ccagagtctg aacagtcttt
    21721 tgtcgccgtt gcgtgaacaa ctggacggtt ttcgccgtca ggttcaggac agtttcggta
    21781 aagaagcaca agaacgtcat accctgactc acgaaatccg caatctccag caactcaacg
    21841 cgcaaatggc ccaggaagcg atcaacctga cgcgtgcgct gaaaggcgac aataaaaccc
    21901 agggcaactg gggcgaagtg gtgctaaccc gcgtactgga ggcgtctggt ctgcgtgaag
    21961 ggtatgaata tcagactcaa gtgagtatcg aaaccgacac tcgttcgcga atgcaaccgg
    22021 atgtcatcgt gcggctgccg cagggcaaag atgtggtgat cgacgccaaa atgacgctgg
    22081 ttgcctatga acgttatttc aacgctgaag atgactacac acgtgaaagc gcgctacagg
    22141 aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc
    22201 tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt
    22261 tactggcgct tgaccgtcag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc
    22321 tggttagccc gactacgctg ctggtggcgc tgcgcactat cgccaacctg tggcgttatg
    22381 agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga
    22441 tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata
    22501 actatcgcca ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag
    22561 aagcgtttcg cggtttaggg gtagaaatta aacgcgagat taatccggat ttggctgaac
    22621 aggcggtgag ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg
    22681 aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt gggggtagtt
    22741 aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt
    22801 tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag
    22861 accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca
    22921 aaatacgatg tcatgaatga tttgatgtca ttcggtattc atcgtttgtg gaagcgattc
    22981 acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc
    23041 ggcgacctga cagcgaaatt ctctcgcctg gtcggagaaa ctggcaaagt ggtccttgct
    23101 gatatcaatg aatccatgct caaaatgggc cgcgagaagc tgcgtaatat cggtgtgatt
    23161 ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt
    23221 gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt
    23281 tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca
    23341 attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt
    23401 ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg
    23461 catcccgatc aggataccct gaaaaccatg atgcaggatg ccggattcga aagtgttgac
    23521 tactacaatc tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag
    23581 accggaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt
    23641 cctgtatcgc tcacccgcgc tgaaaacggc ccgctcgcgt ctgttgggca aagtgctgcg
    23701 cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggtcga
    23761 tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcttacg ccagcgtgtt
    23821 gccgaaactt cgcgatcgcc agcagcttgc cgcactgatt cgtagtggtg agctggaagt
    23881 gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg ctgagttcga
    23941 ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggta tcagcaaagc
    24001 cctgcgcggg ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc
    24061 ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc
    24121 ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact
    24181 ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta
    24241 agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg
    24301 cgatactcat tattctggat gccaaatcgg cataaagaca aacctttagg tgagcgacta
    24361 cgactggccc tgcaagaact gggaccagta tggatcaagt tcgggcagat gttatcaacc
    24421 cgccgcgatc tttttccgcc gcatattgcc gatcagctgg cgttattgca ggacaaagtc
    24481 gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg
    24541 gtagaagcgt ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag
    24601 gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt tatccgcccg
    24661 gatattttgc cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg
    24721 ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa
    24781 aagaccttga ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcga
    24841 cgcaattttg aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt
    24901 gaagggatga tggtgatgga gcgtatttac ggcattccgg tgtctgatgt tgcgacgctg
    24961 gagaaaaacg gcaccaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact
    25021 caggtctttc gcgacagctt tttccatgct gatatgcacc ctggcaacat cttcgtaagc
    25081 tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta
    25141 aacaaagaag ataaacgcta tctggcggaa aactttatcg ccttctttaa tcgcgactat
    25201 cgcaaagtgg cagagctaca cgtcgattct ggttgggtgc caccagatac caacgttgaa
    25261 gagttcgaat ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa
    25321 atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa
    25381 gtgcagccgc aactggtgtt actccagaaa accctgctct acgtcgaagg ggtaggacgc
    25441 cagctttatc cgcaactcga tttatggaaa acggcgaagc ctttcctgga gtcgtggatt
    25501 aaagatcagg tcggtattcc tgcgctggtg agagcattta aagaaaaagc gccgttctgg
    25561 gtcgaaaaaa tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat
    25621 ttacagcata gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga
    25681 caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg
    25741 gtcagccgac ctgaatgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc
    25801 tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa
    25861 cgtataatgc ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc
    25921 agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag
    25981 ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc
    26041 gatgatgaac caaagcagga taaaaccagc caggatgctg attttactgc gaaaactatc
    26101 gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac
    26161 gataaagagc aggtgtaatc tgtgtttgat atcggtttta gcgaactgct attggtgttc
    26221 atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg
    26281 ggctggattc gtgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag
    26341 ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac
    26401 ctgacgcccg aactgaaagc gtcgatggat gaattacgcc aggctgcgga gtcgatgaag
    26461 cgttcctacg ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac
    26521 ccggtggtga aagacaatga aactgtgcat gaaggcgtaa cgcctgctgc tgcacaaacg
    26581 caggccagtt cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct
    26641 gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca
    26701 tgtctgtaga agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc
    26761 tgaactgcat tatctcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca
    26821 tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg
    26881 ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga
    26941 ttctgtcagc gccggtgatt ctctatcagg tgtgggcgtt tatcgcccca gcgctgtata
    27001 agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg
    27061 gcatggcgtt cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg
    27121 cgccggaagg ggtacaggta tccaccgaca tcgcgagcta tttaagcttc gttatggcgc
    27181 tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga
    27241 tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat
    27301 tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgctg ttggcgatcc
    27361 cgatgtactg cctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc
    27421 gaaaccggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc
    27481 aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt
    27541 gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg
    27601 agttaatggg ctactcatca ccggtaccaa tctgcgtgaa agccagcagg cgcaaaagct
    27661 ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca
    27721 gtggcaagct gtgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc
    27781 gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg
    27841 cgcttttgtt gcccagctac gcattgccgc agaattaaac atgccggtat ttatgcactg
    27901 tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg
    27961 tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgtgtgg
    28021 aatttatatc ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga
    28081 attgttgccg ttgattccgg cggagaaatt gctgatcgaa actgatgcgc cgtatctgct
    28141 ccctcgcgat ctcacgccaa agccatcatc ccggcgcaac gcgccagccc atctgcccca
    28201 tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac
    28261 ggatgccaat gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt
    28321 ctttacgctg tgcttaattt ctttattgat aagattaagc aatagcatgg agcgagcctc
    28381 tccgtctggc tcggtgaaaa tggcctgaaa accttcgaat gcgccttcag taataatcac
    28441 tttatcacca ggatacggcg tcgatggatc gacaatatct ttcggtttat acactgataa
    28501 ctgatgaatc accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtggct
    28561 gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tccggatcga actccacaaa
    28621 caagtagttg gggaacaatg gctcactgac tgcagtacgt ttgccacgca cgattttttc
    28681 cagggtgatc atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg
    28741 ttgaagttgc ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg
    28801 tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt
    28861 gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg
    28921 ccgtataatg ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg
    28981 cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga
    29041 tccgcacctg gaaatcactg aaattgctga ccgcactttg cgtgccggtg ggcctgcgct
    29101 gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc
    29161 aaagcgcgtg gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa
    29221 attattggcg tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa
    29281 actgccgcag tttaagcaag tattgaacat gccgacaaag cgactgcgtg gtgcaccctg
    29341 ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg
    29401 ctggccggaa gatgccgcgc cgctgattac ctgggggctc accgtaacgc gtggcccgca
    29461 taaagagcgg cagaatctgg gcatttatcg ccagcagcta attggtaaaa acaaactgat
    29521 tatgcgctgg ctgtcgcatc gcggcggcgc gttggattat caggagtggt gtgcggcgca
    29581 tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg
    29641 tgcagtcacc cccgttccgg atacgctttc agagtatgcg tttgccggat tgctgcgcgg
    29701 taccaagacc gaagtggtga agtgtatctc caatgacctt gaagtgcccg ccagtgcgga
    29761 gattgtgctg gaagggtata tcgaacaagg cgaaactgcg ccggaagggc cgtatggcga
    29821 ccataccggt tactataacg aagtcgatag ttttccggta tttaccgtga cgcatattac
    29881 ccagcgtgaa gatgcgattt atcattccac ctataccggg cgtccgccag atgaacctgc
    29941 ggtactgggt gtagcactga acgaagtgtt cgtgccgatt ctgcaaaaac agttcccgga
    30001 aattgtcgat ttttatctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat
    30061 caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg
    30121 ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cccgcgactg
    30181 gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt
    30241 agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa
    30301 aatggggctg gatgccacga ataaatggcc gggtgaaacc cagcgtgaat ggggacgtcc
    30361 catcaaaaaa gatccagatg ttgtcgcgca tattgacgcc atttgggatg aactggctat
    30421 ttttaacaac ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca
    30481 gagaaagcgc atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac
    30541 cgtatatcgt gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt
    30601 gatggtagtg atggatgagc gcgacaaacg tccgttctca atggcttcga cgccagatga
    30661 aaaagggttt atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt
    30721 catggaccgc atcctcaaag atcatcaaat cgtggtcgat attccccacg gagaagcgtg
    30781 gctgcgcgat gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta
    30841 tgcccgctcg attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta
    30901 ctggggcggg cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt
    30961 gaagcatcct ggtctacaag tggtgccggt ggttgaacaa ccggaagcgg gctggcgtgg
    31021 gcgtactggc accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga
    31081 tatctatatt gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagcga
    31141 gcgtaatgcg cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa
    31201 aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttatacagt ggcattactc
    31261 tacgttgcat ccggcaaaca gtgcccgatg caacgctgcc gcgtcttatc gggcctacaa
    31321 aagttctgaa ccgtgggccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    31381 gctcaaacac cgttgcaatg ccctgaccca gaccgataca catcgtcgcc agaccaaact
    31441 ggacgtcttt gtgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    31501 ccagcggatg acccagcgcg atcgcgccac cgttgaggtt gatcttctcg tcaatctgct
    31561 ccattagccc gagatcttta atacacggca ggatctgcgc ggcaaaggct tcgttcatct
    31621 cgaatacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    31681 gaaccggacc ataacccata atagatgggt cacaacccac aaccgccatc gaacgcacgc
    31741 gggcgcgcgg cttaagacct aattcacggg cgcggctttc actcatcacc agcatggcag
    31801 ctgcgccatc ggaaagcgca gaagatgtac ccgccgttac cgtaccgctg acgggatcaa
    31861 acgccggacg cagtgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    31921 aattaaactg cttcaatacg ccgtcggcat cgtgaccacc ggtcgggatg atttcatttt
    31981 taaatgcgcc tgactgcgtg gcggcccacg cgcgggcgtg tgaccgcgcg gcaaaggcat
    32041 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    32101 tgcccgccgc tttggcgaca ttacggctca ggccggggtg aaaatcgacg ccgtgactca
    32161 ttggcacatg gcccatatgc tccacgccgc caaccagaca tgcctgcgca tcaccggtca
    32221 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattaacgg
    32281 taaccgccgg gacagagtgc ggcacttcag ccaacagtgc cgcgttacgg gcgatattaa
    32341 aaccctgctc cagtgtttgc tgcacacaac cccagtaaat atcgtcaagg gccgccgctt
    32401 ccagtgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    32461 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    32521 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    32581 gctcaaccgg gggatagtac ggttcgttat gacgtgcttt attacgcaga ccttccggta
    32641 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctgc
    32701 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    32761 ccagcgccat atccgcttct gccggagtgg cgataatgcc ctcttccaga cagcggacca
    32821 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    32881 tcggctggct cacttttgcc agcaggtcgt caacgacgac atcttcttct ttcttcggct
    32941 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    33001 tggcatcaaa cagcgcgtcg atggcatcgc gataatcttt ctgcatccgc tgcgggaagc
    33061 ctgctgccat gacagcctgg gcgtgatgcg cggtgtcaat gcccacaaca tccagcagat
    33121 atgccggacc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    33181 ccgcgccgtc gcgcagcaac tggctgaaac cggcgaaata ggggaacagt acacggttga
    33241 cgaagaagcc ggggcagtcg ttaaccacaa tcggcgtttt gcccatcttg ctcgcccagg
    33301 cgacgacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    33361 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccgga cgttccagcg
    33421 cgttggccag ttcgctgata ggaatggttg aagtgttcga cgccagtacg gtatccgggc
    33481 gtactttctg ctcggtttct gccagcacgg cttttttcac tttcgggttt tcaacaaccg
    33541 cttctaccac aacatccacg cggtcaaacc cggcgtagtc gagcgttggg tgaattgtgg
    33601 agatcacgcc agccagtttc agaccatcga tcttgccgcg ctcaagctgc ttgttcagca
    33661 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    33721 ccggcacgcc tttccacgca gactggtaag cgatgccgcc gcccataatg cctgcgccca
    33781 gcacagcggc ctgtttcggg gtttcaacat ctttggtgag tttcttcgct ttacctttta
    33841 catattgatc gttaaggaaa atgccgacca gtgcgcgggc ttcgttggta tgcgccagcg
    33901 ggacaaaact tttgttttcc aggtttaagg cttcttcacg accaaaacgg gccgcagctt
    33961 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgtgcgacca
    34021 tccctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcca
    34081 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    34141 ccatcgcccc ttcaatcagt ttttctgctt tgactacacc atccaccaga ccgattttca
    34201 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagcgca ctgtcagcgc
    34261 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    34321 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    34381 cgcagccacc gcccagcgca tagccgttga cggcagcaat ggtcggcacc gggagatctt
    34441 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    34501 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    34561 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    34621 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    34681 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    34741 tctcctgaat ccgcaaggtg atctggtcgt accagatgca tcgaagtgtg tattttgtgt
    34801 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    34861 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    34921 aaaaatggaa tcactggcct cactctataa aaatcatatc gctaccttac aagaacggac
    34981 tcgcgatgcg ctggcgcgct tcaagctgga tgcgttactt attcactccg gcgaactgtt
    35041 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    35101 ggtgccggta actcaggtgc caaactgctg gctgctggtg gatggcgtga acaagccgaa
    35161 actgtggttt tatctgccgg ttgattactg gcacaacgtc gaaccgctac cgacctcctt
    35221 ctggactgaa gatgtagaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    35281 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    35341 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attactaccg
    35401 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    35461 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaacat
    35521 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttatagca acattgtggc
    35581 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    35641 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgatctgac
    35701 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    35761 tgagcaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccatat
    35821 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    35881 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtatcggcca
    35941 tccgctgggc ctgcaggtgc atgacgtagc cggttttatg caggatgata gcggtacgca
    36001 cctcgcggca ccggcaaaat atccgtacct gcgctgcacc cgtattctcc agccgggcat
    36061 ggtgttaacc atcgaaccgg gtatctactt catcgaatcg ctgctggcac cgtggcgtga
    36121 agggcagttc agcaaacact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    36181 cattcgtatc gaagacaacg tggtgattca cgaaaacaac gtggaaaaca tgacccggga
    36241 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    36301 gagatcaaaa agagccgctt cattacgctg ttggcgcata ccgatggcgt tgaggcggca
    36361 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    36421 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    36481 gcgggaacgg caggtaaacc gatgctcgcc caactaatgg gcagcggcgt cggggaaatt
    36541 accgctgtgg tggtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    36601 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacaccg
    36661 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    36721 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    36781 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    36841 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    36901 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtt atcttatttt
    36961 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    37021 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctttgg tggccgaacc
    37081 gcaaagagaa gggtgaactg aaatcccgtg aggggtttct gatagtggtg ctgttctgga
    37141 ccgtgctggg tagcgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    37201 ttaccgatgc gttttttgaa tctttctctg gcctgaccac cacgggggcc actacgctgg
    37261 tggggctgga ttcgctccct cacgccatcc tcttttatcg ccagatgctg caatggtttg
    37321 gcgggatggg gatcatcgtg ttggcggttg cgatactgcc tatcctcggc gtgggtggga
    37381 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    37441 ttgcggaaac ggcgaaaacc ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    37501 tggcgttgtg gtttgctgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    37561 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agcccgacta
    37621 ttaacactat cattgctatc ttcctgctga tctctggctg taactacggt ctgcactttt
    37681 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    37741 tcggcgtaca gttttcactg gtggttattt gtacactcgt actgtggttt cataatgtct
    37801 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtgtcg atggcgacaa
    37861 ccgccgggtt tacaactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    37921 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    37981 gcatcctgtt gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    38041 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    38101 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    38161 cgggcgtgga tgacttttct gcctttgcct cggttgttgc aacattgaat aacctggggc
    38221 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    38281 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attgctggtg ctctttaccc
    38341 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    38401 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    38461 ccaggcggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    38521 tgtggttatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    38581 caaaaagcat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaatctggt
    38641 ggcgcgtaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    38701 gaactcgcaa tggcgtcccg atcgctgtgc cgtcattgcc ggggcgctgc gttacccacg
    38761 ttatcgctgg tacgaccgtt ttatgatcaa gctgattatg aagatgtcag gcggtgaaac
    38821 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    38881 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    38941 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    39001 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaatct
    39061 caacagagaa aagcaaaaaa atgcttgact ctgtagcggg aaagcgtatt atgca
//
LOCUS       DAPQZD010000025        38962 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPQZD010000025 DAPQZD010000000
VERSION     DAPQZD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.025"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(726..1763)
                     /locus_tag="RO454_001289"
     CDS             complement(726..1763)
                     /locus_tag="RO454_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDY0933154.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            complement(1816..1910)
                     /locus_tag="RO454_001290"
     tRNA            complement(1816..1910)
                     /locus_tag="RO454_001290"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1874..1876),aa:Sec,seq:tca)
     gene            2203..3585
                     /locus_tag="RO454_001291"
     CDS             2203..3585
                     /locus_tag="RO454_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDY0933155.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3595..5913
                     /gene="yicI"
                     /locus_tag="RO454_001292"
     CDS             3595..5913
                     /gene="yicI"
                     /locus_tag="RO454_001292"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDY0933156.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            6208..7761
                     /locus_tag="RO454_001293"
     CDS             6208..7761
                     /locus_tag="RO454_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDY0933157.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVDYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            7788..8258
                     /locus_tag="RO454_001294"
     CDS             7788..8258
                     /locus_tag="RO454_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDY0933158.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8276..8590
                     /locus_tag="RO454_001295"
     CDS             8276..8590
                     /locus_tag="RO454_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDY0933159.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8615..9706
                     /locus_tag="RO454_001296"
     CDS             8615..9706
                     /locus_tag="RO454_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDY0933160.1"
                     /translation="MKENKITVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            9718..10569
                     /locus_tag="RO454_001297"
     CDS             9718..10569
                     /locus_tag="RO454_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDY0933161.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            10650..11510
                     /locus_tag="RO454_001298"
     CDS             10650..11510
                     /locus_tag="RO454_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDY0933162.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            11558..12490
                     /locus_tag="RO454_001299"
     CDS             11558..12490
                     /locus_tag="RO454_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDY0933163.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            12471..13151
                     /locus_tag="RO454_001300"
     CDS             12471..13151
                     /locus_tag="RO454_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDY0933164.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(13195..14904)
                     /locus_tag="RO454_001301"
     CDS             complement(13195..14904)
                     /locus_tag="RO454_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDY0933165.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLKNVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFAPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15025..16416)
                     /gene="xanP"
                     /locus_tag="RO454_001302"
     CDS             complement(15025..16416)
                     /gene="xanP"
                     /locus_tag="RO454_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDY0933166.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16696..17901
                     /gene="gltS"
                     /locus_tag="RO454_001303"
     CDS             16696..17901
                     /gene="gltS"
                     /locus_tag="RO454_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDY0933167.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(17935..20016)
                     /gene="recG"
                     /locus_tag="RO454_001304"
     CDS             complement(17935..20016)
                     /gene="recG"
                     /locus_tag="RO454_001304"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDY0933168.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20022..20711)
                     /gene="trmH"
                     /locus_tag="RO454_001305"
     CDS             complement(20022..20711)
                     /gene="trmH"
                     /locus_tag="RO454_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDY0933169.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(20718..22826)
                     /gene="spoT"
                     /locus_tag="RO454_001306"
     CDS             complement(20718..22826)
                     /gene="spoT"
                     /locus_tag="RO454_001306"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDY0933170.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(22845..23120)
                     /gene="rpoZ"
                     /locus_tag="RO454_001307"
     CDS             complement(22845..23120)
                     /gene="rpoZ"
                     /locus_tag="RO454_001307"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDY0933171.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23175..23798)
                     /gene="gmk"
                     /locus_tag="RO454_001308"
     CDS             complement(23175..23798)
                     /gene="gmk"
                     /locus_tag="RO454_001308"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDY0933172.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24056..25738
                     /gene="ligB"
                     /locus_tag="RO454_001309"
     CDS             24056..25738
                     /gene="ligB"
                     /locus_tag="RO454_001309"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDY0933173.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(25735..26352)
                     /locus_tag="RO454_001310"
     CDS             complement(25735..26352)
                     /locus_tag="RO454_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDY0933174.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26604..27467)
                     /locus_tag="RO454_001311"
     CDS             complement(26604..27467)
                     /locus_tag="RO454_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDY0933175.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27594..28310
                     /gene="rph"
                     /locus_tag="RO454_001312"
     CDS             27594..28310
                     /gene="rph"
                     /locus_tag="RO454_001312"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDY0933176.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            28376..29017
                     /gene="pyrE"
                     /locus_tag="RO454_001313"
     CDS             28376..29017
                     /gene="pyrE"
                     /locus_tag="RO454_001313"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDY0933177.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(29054..29650)
                     /gene="slmA"
                     /locus_tag="RO454_001314"
     CDS             complement(29054..29650)
                     /gene="slmA"
                     /locus_tag="RO454_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDY0933178.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(29757..30215)
                     /gene="dut"
                     /locus_tag="RO454_001315"
     CDS             complement(29757..30215)
                     /gene="dut"
                     /locus_tag="RO454_001315"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDY0933179.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(30193..31413)
                     /gene="coaBC"
                     /locus_tag="RO454_001316"
     CDS             complement(30193..31413)
                     /gene="coaBC"
                     /locus_tag="RO454_001316"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDY0933180.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31585..32253
                     /gene="radC"
                     /locus_tag="RO454_001317"
     CDS             31585..32253
                     /gene="radC"
                     /locus_tag="RO454_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDY0933181.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            32470..32706
                     /gene="rpmB"
                     /locus_tag="RO454_001318"
     CDS             32470..32706
                     /gene="rpmB"
                     /locus_tag="RO454_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDY0933182.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            32727..32894
                     /gene="rpmG"
                     /locus_tag="RO454_001319"
     CDS             32727..32894
                     /gene="rpmG"
                     /locus_tag="RO454_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDY0933183.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            32992..33801
                     /gene="mutM"
                     /locus_tag="RO454_001320"
     CDS             32992..33801
                     /gene="mutM"
                     /locus_tag="RO454_001320"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDY0933184.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(33840..34319)
                     /gene="coaD"
                     /locus_tag="RO454_001321"
     CDS             complement(33840..34319)
                     /gene="coaD"
                     /locus_tag="RO454_001321"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDY0933185.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(34327..35604)
                     /gene="waaA"
                     /locus_tag="RO454_001322"
     CDS             complement(34327..35604)
                     /gene="waaA"
                     /locus_tag="RO454_001322"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDY0933186.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            36018..37076
                     /gene="rfaQ"
                     /locus_tag="RO454_001323"
     CDS             36018..37076
                     /gene="rfaQ"
                     /locus_tag="RO454_001323"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDY0933187.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            37073..38197
                     /locus_tag="RO454_001324"
     CDS             37073..38197
                     /locus_tag="RO454_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDY0933188.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            38190..>38962
                     /gene="rfaP"
                     /locus_tag="RO454_001325"
     CDS             38190..>38962
                     /gene="rfaP"
                     /locus_tag="RO454_001325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDY0933189.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLLNMASVKAEHIK"
BASE COUNT         9328 a         9469 c        10374 g         9791 t
ORIGIN      
        1 attgttagcg caagtttgag ttgctagcca ggatgccgct ggatgactca aattaatatg
       61 tgtgctaaag actttgacgc gactacttct gtttttctct ttactttcaa ttaaatgcct
      121 agcgctgttg gctgctttac aagttccatg ccccacacgg caaaaataat ctcctgaaag
      181 gatacttaca atcagtatta caccaatgac gacagaaaaa atagttccga tgagtttgat
      241 gatgacaatc cttgtgcagt gttattttca ccatggatta taatgttata gtgaaattat
      301 tgcacctgta atttttcttt gattgtttta atcatcacat ttacgcactt cttcatcttt
      361 gtagcaggta aaagccatat atggatggct ggtgtccggt gcagataaaa tgcaacttat
      421 tcttgatagc ttgaaaaaag agaggttttt ttgagtgacg gttgatggtc ggtggcaagg
      481 aagacgtttt cgtacactcg gcaagaaaaa tgaatcactc tgtcatacta ccagatacat
      541 cagcagggtc aggttgctca gaaataagca ggatggggat cactgaagag gcattttggc
      601 gatcacagga gtcgcatttg atggttaagt tattgattta atgtttatat tttgaacttg
      661 ataaatgagt aaccccataa gtacccccaa atggttttgc gtatggcgtt tggggggatt
      721 ccgggctact ttttcgactc tttattccac tgtagcaaac cagcgatatc cttctcgcct
      781 tcggcttctt ttaagcgccg ttgttgttcg gcaaactgaa catactccgc ctgcgctttt
      841 tcatcggcca ttttcttgct gattttacca gcgccctgta gaacctctcg gtcgttaaat
      901 tgcaggaact gatccagctt atcctgccag tcgcgcaaaa agacttgctg acgacgacgg
      961 gcctgatcct cggcaaagtc cagccacata ttaaccacgc gattaagttc gctgacctca
     1021 tcctgggtga gataattttt tgccaccgtc acgtcgccct tacgtacctc ttcaccttta
     1081 taactggtca agcccatgtg cggctggctg gcatctgctc gtttgtggat gagttccgca
     1141 gcggtatgcc cggtacaggc aaaatgcagt ttattctgga tggtttgaaa aaattgcgtg
     1201 gtttctttaa gcgatggctg atagtcggca gctaaagcaa agatctcccg cacccgcaaa
     1261 tatacccgac gctcgctggc gcgaatatcg cggatgcgtt caagcatctc atcaaaatag
     1321 tcgggcacaa ccgatgaacc gacaggcgga ttcttcagcc gctcatcgtc catcacaaaa
     1381 cctttgatca ggtattcctg aagcgtctgg gtggcccact ggcggaattg cgtgcctcga
     1441 ggcgagcgaa cgcgatagcc gacagcgaga atggcttcga ggttgtaatg gtctatctct
     1501 ctggaaacct gacgttttcc ttcctggcga actatccgga atttccggat ggttgagttt
     1561 tgaacaagtt caccttcgga aaatatattg attaaatgtt cgttaatagt gcgaacatct
     1621 ttatcgtaca gttcagccat tgccgcctgg gataaccaga ttgtgtctga ctcaaagcga
     1681 cattcaacac gcactcttcc gtcaccgctg gcaaacataa taaactcgcc tgctgggggc
     1741 tgggttaagt ctttgtctga catgttgcct ccgaaggagt gttgttgacg tcgggagagt
     1801 tcaggagagg cattttggcg gaagatcaca ggagtcgaac ctgcccggga ccgctggcgg
     1861 ccccaactgg atttgaagtc cagccgcctc accggagacg acgatcttcc gcgcctcgat
     1921 tgctacatgg aggcggggcg cattatagct acttccttga gtttctacat cccccagatc
     1981 agttttttcc gcctaataca cgcagcgtat tatcacgaaa gcattatagt tcatataaaa
     2041 ctcatatttt acaatgatat atgagcgaaa ttaacgctac cacgatcaca taaaacaaga
     2101 atcataaatt aataaccaga tatcggaata ttcgctctcg cagggatggt ttacaaaatg
     2161 cgttatcggt ctcagcaccc ctatagcatc aaggaaaagc agatgaagag tgaagtgttg
     2221 tccgttaaag agaaaattgg ttatggcatg ggagacgccg ccagccacat tattttcgat
     2281 aacgtaatgt tatatatgat gttcttttat accgatattt ttggcattcc tgccggattt
     2341 gtcggaacca tgtttttggt cgctcgtgca ctggatgcga tttccgatcc ttgcatggga
     2401 ttgttggccg atcgaacgcg ctctcgctgg ggtaaatttc ggccgtgggt actgtttggt
     2461 gcactgccat tcgggatcgt ctgtgtacta gcctatagca cgccagatct cagtatgaac
     2521 ggaaaaatga tttatgcagc aattacttac accctactta ccttacttta taccatcgtc
     2581 aatatccctt actgcgcatt gggtggtgta atcaccaatg acccgactca gcgtatctcg
     2641 ctgcaatcct ggcgttttgt gctggcgacc gcgggaggca tgctctctac tgttctgatg
     2701 atgccactgg ttaatttaat tggtggtgat aataaaccac tcggtttcca gggcggtatc
     2761 gcggtccttt ccgtggtggc atttatgatg ctggcattct gtttcttcac cactaaagaa
     2821 cgcgttgaag caccacctac aacgacgtct atgcgggatg atttacgtga tatctggcaa
     2881 aacgaccagt ggcgtattgt cggtttcctg accattttca atatcctggc ggtgtgcgta
     2941 cgtggtgggg cgatgatgta ttacgtcaca tggattttgg gtacgccgga agtatttgtt
     3001 gcttttctca ccacttattg cgtgggtaac ctgattggtt ccgcactggc aaaaccgctg
     3061 accgactgga aatgtaaagt cactatcttc tggtggacga acgccctgct ggcagtgatt
     3121 agcctcgcga tgttctttgt tcctatgcag gccagcatca ccatgtttgt cttcatcttc
     3181 gtgattggtg tgttgcatca actggtgaca cctatccagt gggtaatgat gtccgatacc
     3241 gtcgactacg gcgagtggtg taatggtaaa cgcctgaccg ggatcagttt tgctggcacg
     3301 ctgtttgtgc tcaaactggg gttggccttc ggtggtgccc ttatcggctg gatgctggct
     3361 tatggcggat atgatgcggc agaaaaagcg cagaacagcg ccacaattag catcattatc
     3421 gcgctattca cgattgttcc ggcgatctgt tacttgctga gcgcgattat cgctaaacgc
     3481 tactactcgc tcacgacgca caatctgaaa accgttatgg aacagctggc gcagggcaaa
     3541 cgccgttgcc agcaacaatt cacctctcaa gaagtgcaga actaaggaac ggcaatgaaa
     3601 attagcgacg gaaactggtt gattcaacct ggcctcaatt tgattcaccc gcttcaggtg
     3661 ttcgaggttg aacagcaggg taatgaaatg gtggtctatg ctgcccctcg tgatgtgcgt
     3721 gaacgtacct ggcagcttga tacgccttta tttacgctgc gctttttctc cccacaggaa
     3781 ggtattgtcg gtgtacggat tgagcatttt cagggggcgc tgaataacgg tcctcattac
     3841 ccgctcaata ttttgcagga cgtgaaggtc actatcgaaa acacagaacg ttacgctgag
     3901 tttaaaagtg gcaacttaag tgcgcgtgtc agcaaaggtg agttctggtc actggatttt
     3961 ctgcgcaacg gcgaacgtat taccggtagt caggtgaaaa ataatggcta cgtgcaggac
     4021 acgaataatc aacgaaatta tatgtttgaa cggctggatc ttggcgttgg cgaaacagtt
     4081 tacggcctgg gagagcgctt tactgccctg gtgcgcaatg gtcagacggt agaaacctgg
     4141 aaccgggacg gcggcacaag tactgaacag gcgtataaaa atatcccgtt ttatgtgact
     4201 aaccgtggtt atggggtact ggtcaatcat ccccaatgtg tctcttttga agtgggatcg
     4261 gagaaagtct ccaaagtaca gttcagcgtt gagagtgaat atctcgaata ctttgttatc
     4321 gacggcccga cgcctaaagc ggtacttgat cgttataccc gttttactgg ccgtccggcg
     4381 ctgccgcccg cgtggtcctt cggtctgtgg ctaaccactt catttaccac caactacgac
     4441 gaagcgacgg taaacagctt tatcgatggt atggcggaac gcaatctgcc gctgcatgtt
     4501 ttccactttg actgtttctg gatgaaagcc ttccagtggt gcgattttga gtgggatccg
     4561 ctgacttttc cggacccgga agggatgatc cgccgcctga aagcgaaagg gctgaaaatc
     4621 tgcgtctgga ttaaccccta tattgggcaa aaatcccccg tctttaaaga gttacaagag
     4681 aaaggttatt tactcaaacg cccggacggt tcgctgtggc agtgggataa atggcagcca
     4741 ggtctggcga tttatgactt taccaatccg gatgcctgca aatggtacgc cgacaaactg
     4801 aaaggtctgg tcgcgatggg cgttgattgc tttaagaccg actttggcga acgtattcca
     4861 accgatgttc aatggtttga tggttccgat ccgcagaaaa tgcataacca ttatgcatac
     4921 atctacaacg aactggtgtg gaacgtgctc aaggacaccg ttggtgagga agaagccgtc
     4981 ttgtttgccc gctcggcctc cgttggtgcg cagaaatttc cggtacactg gggtggcgac
     5041 tgttacgcta actacgaatc aatggcggaa agcctgcgcg gtggtttgtc tattggcctt
     5101 tcaggttttg gcttctggag ccacgatatc ggcggctttg aaaataccgc tccggcgcac
     5161 gtttacaaac gctggtgcgc gtttggtttg ctctccagcc atagccgttt acatggcagc
     5221 aaatcttatc gtgtgccgtg ggcctacgat gatgagtcct gtgatgtggt gcgcttcttc
     5281 acgcaactga aatgccgcat gatgccgtat ctgtatcgtg aagctgcacg tgcaaacgcc
     5341 cggggtacgc cgatgatgcg ggccatgatg atggagttcc cggacgatcc ggcttgtgat
     5401 taccttgacc gtcaatacat gttaggcgac aacgtgatgg ttgcgcctgt gttcactgaa
     5461 gcgggcgatg tgcagttcta cctgccggaa ggtcgctgga cacacctgtg gcacaacgat
     5521 gaactcgacg gtagtcgctg gcataaacag cagcacagct tcctgagtct gcccgtttat
     5581 gtgcgtgata gcaccctact ggcgctgggc aacaacgatc aacgtcccga ttacgcgtgg
     5641 cacgaaggta cggcattcca cctctttaat ctgcaagacg ggcatgaagc cgtctgtgaa
     5701 gtgcccactg ctgacggttc ggtgatcttt actttaaaag cagcacgtac tggcaacacg
     5761 attactgtga ctggtacggg cgaggcgaag aactggacac tgtgcttgcg caatattgtg
     5821 aaagtaaatg gtctgcaagg cggttcgcag gctgaaagtg agcagggact ggtggtgact
     5881 cctcaaggga atgcgctgac aattacgttg taatctattg aagaatgccg gatacgaatg
     5941 gttttgtgtc gtatccggta aataatctca catctggtaa taaatatttc ttccatcgta
     6001 tttgcgagca tgatcacgat tattcatcac gaaaatgtga tctgtgctga aaaatatccc
     6061 ttaaccgcct tgctttgttt tatcactgtc cggattacgg acagatttta gtattgttgt
     6121 gaaattattc tgctttatat ttaccctcag agatggcaac tgaagctctt ccatatgggg
     6181 cttcttaatc tgtgataagg gtaaaaaatg caaatgatta cctcgcgaca aaacagatta
     6241 ttacgattcc ttttacctcg aagggaatat acgactattg ttacgattgc cggctattta
     6301 aatgtttcgg aaaaaaccat tcaacgtgat ttacgtttac ttgagcaatg gctgggacaa
     6361 tggagaataa atgttgagaa gcgtgctggc gcgggtgtga tgttaagcgc ggagaatatt
     6421 gctgatttgc tgcatcttga tcatttgttg gtggcagaat gtgaagagat tgatggtgta
     6481 atgaataatg ccaggcgcgt taaaatagcg tcgcagttat taagtgaaac accgaatgaa
     6541 acgtcgatca gtaaattgtc agaacgctat tttatcagcg gagcctctat tgttaatgac
     6601 ctgagagtaa ttgagtcctg gcttgcgccg ttggggttat cgttgatccg cagcccaagt
     6661 ggtacgcata ttgaaggtag tgaagggcaa gtccgacagg caatggcatt actgattaac
     6721 ggcattatta accataatga gccgcaaggt gtcgtgtatt cacgtctgga tcccggaagc
     6781 tataaagcat tagtccatta ttttggagag gaagaagtgt tatttgtcca gtcattgtta
     6841 ctggatatgg aaaatgaatt aagttggtct ttgggagaac cttattacgt taacattttt
     6901 actcatatcc ttattatgat gtatcgcaac acgcacggga atgcgttatc aagagaagaa
     6961 gatcaaacca ggcaatatga tgaaaatatc tttaatgttg ccagtcagat gattcataag
     7021 atagaacaac gaattgcaca tacattgccc gatgatgaag tctggtttat ttatcaatat
     7081 atcatttcat caggtgtggc gattgatgga caaaaagatg ttagcattat ttcacatatg
     7141 caggccagca atgaagcgcg tctgattacc tggcgtttaa ttacggtatt cagtgacatc
     7201 gtggactgcg attttagtga agacagcgca ttatatgatg gcttattggt gcatattaaa
     7261 ccgctgatta accgactaaa ttatcgtatt catatccgta atccattgct ggaagatatt
     7321 aaagcagaat tagcggatgt ctggcggttg acgcaatatg tggtgaatca ggtatttaaa
     7381 acctggggtg agaatgcggt gagcgaggat gaagtggatt acctgaccgt tcattttcag
     7441 gctgcgatgg aacggcaaat tgcccgtaaa cgcgtattac tggtctgttc aaccggaatc
     7501 ggaacttcgc atctactgaa aagccgtatt ctgcgagcat ttcctgaatg gacgattgtt
     7561 gatgttattt cagcagcgaa tttatcacag gttttgcctg acaatatcga actgattatt
     7621 tcgaccatta atttgcctac agtcactatg ccggtcgctt atgttaccgc tttttttaat
     7681 gatgccgata ttaagcgggt cactgaaatg gtgattacgg aaaaattaca tcatgcgacg
     7741 tctcgggtcg ttgaaattta atgtcagaat tttatgaggt atgacgaatg gacataacaa
     7801 aaatcctgaa tacgaatcgc gttattttag atatgcaagc gacgaataaa gcggaggcga
     7861 ttgaagaatt aactaatctt ctcaaaaaag atggtgcgat cgattgtcgg gaaaccttta
     7921 ttaaagatgt ctggcagcgc gaagcggaag gctcgactgg ttttgaaaat catattgcga
     7981 tcccgcatgg taaatcttca gctgtgatta atactacgct ggcaattggc cgtacccgcc
     8041 aggatattcc ctgggaaacg ctggatggca gtagcgtgcg ctgtattatt ttatttgcgg
     8101 tacgtcttga agatcaaaat accacccata tccgtttact ttcccaggtc gcgggcgcgc
     8161 tggcggatga tgacatcata gaacaattgt tagttgagac gagtccgcaa aaaatcattg
     8221 atctctttag tcagtatgca caatccgatg tctgctaaat aacagggagt atgtcatgaa
     8281 tattgtctgt gttgccgcct gtacggcggg tattgctcat acctatattg ctcgtgaaaa
     8341 gttaatcaaa ggagcaaaag ctctgggcca taccattaaa gttgaaaccc agggaaccat
     8401 tggtacagaa aatgaattac aggcggcaga tatctctgct gctgacgtcg tgattcttgc
     8461 tgtagatgtg aaaataaaag gagaagaacg cttcaccaat aaacgtattg tgcgagtaaa
     8521 gacagagatc gtcattaagt cgcccgttca atttcttgaa aaggtcgaaa aatcactggg
     8581 aaacaaatga tccttatact ggagggatct taatatgaaa gaaaataaaa ttacggtttc
     8641 acaggaaatt aaaaaacatt tattaaccgg catctcgtgg atgatacccc ttattgtggc
     8701 cgccgggata tgtattgcgt tggggcaagt tcttggcggt acagatgttg gtgaaaaaac
     8761 aggaacaatc ccgtggatgc ttaatcagat tggcggctgg ggaatggggc tgattgtgcc
     8821 gctgattagc gcggcaattg cctattctat tgccgatcgt cccggatttg ccccgggcct
     8881 gattgtcggc tttctctgtg ggcaaatcca taccggattt attggcggta tgttgggtgg
     8941 tttcctggtg ggctacacca ttttgctact taaacgctat atccgcctgc cgcagtcgat
     9001 gcaaggactc atgccaatca tggtgttgcc ggtattaagc accattatcg gtgggctgtt
     9061 aatgatgacg ctgattggta aacccatcgc ctggttacaa gaagcactga ttcatttgct
     9121 cgagtcgatg cagggcggat cgcgcttcct gatgggggcc atacttggcg cgatggcaac
     9181 cttcgatttc ggtggtccgg tcaacaaaac catgtcgctg ttttccgatg gcttgctggt
     9241 cagtggcgtc tacggaccag aagccgtgaa atttgtcggc tcaatcatcc ctccgtttgg
     9301 catcacactt tcgttcttac tgacacgcca taaatacacc cgtgcggagc gcgaggcgct
     9361 gaaagccgcg tttccgatgg ggatctgcat gattactgaa ggagtcattc cgatcgccgc
     9421 gcgtgaccta ctgcgagtag tgggatcttg tgtggtggca tcggcggtcg cgggcggctt
     9481 aatcatggtg tggggcgtgg agagtccggt tccgcatggc ggcatgtttg ttgtgcctct
     9541 gttcacccat cctctgctgt tttgcctggc gctgggtata ggcacggtga tttgtggcgt
     9601 gatgctttca ctctggaaga aaccggtaac ggaacgcgac gaagagtttg acgaactgag
     9661 cgatcaaaag ctgaaagatg aagagatcac tttcaccctc gaataaccgg aggccctatg
     9721 tttgccgata tgaaaagtat ggtgatgaaa gcctggcatg aacattacgc cttgctggcg
     9781 atcaactgta tgaatctgga aagtgctcac gcagctatcc gcgtggcgga gaagaatcgc
     9841 gctccgatca ttctcaatct ctatcagggg catctggcgc attttcctgc acccgtcgcg
     9901 gcggcagtgg ttaaaacgct cgcggaatct gcctcagtac cggtcgctct ggcgcttgat
     9961 catggcaaaa atccggactg tattcgtcag gcgtttcgtg ctggtttcag cggattaatg
    10021 attgatgctt ctgcttttcc cctggaggaa aacgtgcgcc agaccagggc ggtggtggaa
    10081 ctcgccgcca gcgcgcagct ctgcgtggaa ggcgaactgg gccatctggc agatgcgccg
    10141 cgttacgacc aggccgccaa tgcagacctg atgacgcaac ccactgatgt ggaaccgttt
    10201 atcgcgcaga caggcattga tctactggcc gtctcggtag gcacggcgca cggcatgtac
    10261 gcgcctggcg ttgtacctgc tattgatttt cagcgtctgg cggagatttt ccactgctcg
    10321 agcgtgcctc tggcattaca tggcggtagc ggtacgccgt tcgatcagct ccagctctgt
    10381 acgactctgg gggtagcaaa aatcaacgtg ggggcggcca tttttgaacg ggggaaatcc
    10441 gcgctcctgc ataccctctg tcacgatata tccattgaac tggtggacgc gctgaaagct
    10501 atggaactgg ccttcgaaga agcgattaca ccgtatttac aggcgagcgg cgctatcggc
    10561 aaagcctgat tcattttctg tcatggcctt tatgccagtg caataacgca ccggcagggc
    10621 ggactacacg ctgaaactgg agatttaaca tgttagtttc gatgaaagaa ttactgcaac
    10681 ctacccgcca gcacggcttt gccattggcg catttaatgt ggcggataac tgtttccttc
    10741 gcgcagtcgt ggatgaagca gaagcgacaa atactccggc gattattgcc atccatccca
    10801 gcgaacatga ttttgtgggc gatgcctttt tcgctcacgt tcgcgaaatt acccagcgta
    10861 gtccggtccc gtttgtactg cacctcgatc atggtgcctc tgtagagcat gttttgcgtg
    10921 cgattcgctg cggatttacc tcagtaatga tcgacggttc gctgctgccg tatgaagaaa
    10981 acgtggcgct gaccgcagaa gtggttcgcc tcgctcacgc ggttggcgtg tcggttgagg
    11041 gtgaactggg aaccatcgga caaacaggaa cgtcggtaga aggtggtgtt tcacaagtga
    11101 cctacacaga tccggcgcag gccgccgatt ttattgctcg tacaggtgcc gacacactgg
    11161 ctgtggcgat tggtacagcg catggcatct atccaaaagg gatgcagccg aagctgcaaa
    11221 tggacatttt gcgcgacatt gccggacgta ttgatattcc gctggtgcta cacggtggtt
    11281 cggcaaaccc ggacgcggag attgctgaat cagttacgct cggcgtcggc aaaatcaata
    11341 tctccagcga tatgaaatac gcctatttcc agaaagtgcg tgaaattctc gctaaagaga
    11401 gctggtggga cccgaacgtc atctatccgg atgcgattaa cgcggcgcgg gaagtcattc
    11461 gccacaaaat gaaactgttc ggttcgctcg gtaaagcatc actgtactaa gccagaaggg
    11521 cggggtgacc cgccttttac gcaacaagga acgagatatg cactatctgg ggttggatat
    11581 tggtgggacc aaaatcgccg ccgttgtcat ggatgcgcat ggctgggaga ttcgccgtta
    11641 ccgctgcccg acgcaaaagt cgacatatca acaatttgtc tcatgcgttg tggcgcttat
    11701 cgagcagatt aggcgggacg ttcaacgacc gatgctgaca gggatcgcct tacccggcag
    11761 tatctcgcca ctcactggcc tgattaaaaa cgcgaatatt caggtgatta acggtcatgc
    11821 gttacaggct gatttgcagc aattgcttgg gcaaccggtg gtgatagcca atgatggtaa
    11881 ctgtttcgcg ctatcagaag cttgcgacgg tgccgggcaa gattatgacg tggtatttgg
    11941 tattacgctt ggtacgggct gcggcggtgg cattgccatc aagcaacgac cgtttatagg
    12001 ggcctgggga aatgctgccg aatgcggtca tatcacgttg ccaggctata cggagcagga
    12061 agatggtcca tcagtcagtt gctattgcgg caaacacaac tgcgtggagt cgtttgtttc
    12121 cggcagcggt tttagtgaac gctatcaaca gatgactggt aacttgctca ctcctgcggc
    12181 gattgtcacc ctggcacaac gtggtgatgc ttgtgccatg cagcaggtgg cacgttttcg
    12241 ccaacagctt gcccgcacgc tggcaaccat cgttaacgtt gttgaccctg gcgtgattgt
    12301 gatcggcggc gggctttcga atgtggaact gcttatcacc gatctgaacg cagaagtcgc
    12361 tccactggtt ttcaccgatc aattcaccac tcccattgta aaagcacagc acggcgacag
    12421 tagcggaatg cgtggcgctg cctggcttgc tatgcgcaac ggagaagcca atgaaacgtt
    12481 cacaaattaa ttatgttatt gataaagcac atgccattgc gcagaccttc cgcgtttgtt
    12541 tgccagagtt cgcctatttt acggtggatg actggctgca gcgggaacgg gataactggc
    12601 aagaagtggt cgacttacag ctcggttggg atatcaccga ttttggtcgt ggtgatttca
    12661 accaaacggg actaacactg ctaacgatgc gcaacggcgc gctggggtcg gcggcatatc
    12721 caaaaccgta tgctgagaaa atgttgcaga tccaacaaga tcagcagact ccctggcatt
    12781 ttcacaccca caaaatggaa gatatcctca atcggggcgg tggagactta tgcatgcagt
    12841 tggcctgggc caacgaagcc aatctctgtg atgatcaacg agtcattacc gtgagtgttg
    12901 atggtcagcg gcgtaccatg aaacctggtg aaacgctggt gctaaagccg gggcagggca
    12961 tttgcctgcc gccgcgtctg tatcatcgct tctgggcgga aaaagcgttt gttctcggct
    13021 gggaaatctc gatggtaaat gatgaccaac acgataatta ttttcttgag ccgggaggac
    13081 gttttccggc gatagaagaa gatgaaccgg tgaaatggtt attgtgcggt gagtatggaa
    13141 ttttgcgcta ataacgggga acgctagcgc tgatatccgc tagcgtcctt atttttactg
    13201 caccggctcc gtcgacgtaa cttcaccact ggaaactacg cccgcattca tggtttgagt
    13261 cacttgctgc tttgcggcat tcacggcatg caattgcccg ctaaccgtag gtttcagcgg
    13321 gacattgacc tgaatatcgc cactggcggt aagctgaata ttgccgtcgc cagtcaacgg
    13381 taattcaggc catccccaat gttgcaaaat attcaccggc acaccgcgtc cattcaggct
    13441 gatatgggtc tgacgttgtg gcgtttgtga aacactggcg gtggcttcca gaatgccttt
    13501 ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcag ttagcgccag
    13561 cgacggacga cggacatcaa cacgattaaa cgttgcggcg gcggcattca gattcgccga
    13621 gccactccag acgccccatt tatgatcggt aaccagcgtc agattggcac cgtaaccatc
    13681 gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat tgcggctggc
    13741 gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca tccacaactg
    13801 ttgccagttt ttgggcaagg tatattccag tccggcaatt gccgcatcat cgaggatcag
    13861 cgttttcccg tcgcgttgcc agtttcctga cgttctgacc ataccccctt cccagcggct
    13921 ggtgaactgg cgcagtgcta cgccctgcgg ggcaaattcc gcgtttataa tcggatcaaa
    13981 taaatgcagc gagccataaa taaactcgct ggcgttcatc gacagtttac cttcctgtgt
    14041 ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt cggccaccgc
    14101 ccagtccgga ccttgcaaac gagcgtcgat gacttccagg cgaccaatct gcaacgacgg
    14161 gacagagcgt aatggtgcaa agaagtcggt tagcgatttt tcgctttgta gacggatatc
    14221 cgccatgcgc aggttttcca cttgccagct gccgtcagcg ttacgctgcg cgtttccggt
    14281 taatgtcccg cgggcgatgt cggcacccag gttagtcagc gtaacgcgat cgttatcaat
    14341 actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg atccggcact
    14401 aaactgaatc tgtgccttcg tacccagcac tttaccggct tctggtgacc acggaaccac
    14461 gccgccattt acccgctgcg cgctcagttt ccattcgcta ttcgggctat taaacgccat
    14521 atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt cggtgagatt
    14581 cagcgtcccg ttttccagca ggatggtatc gacatggcgt ggttcggtca gttgccgact
    14641 gcttagcgca atgtcgacac tttttgctac cagggtcgcg ggctggccat cacggccaaa
    14701 cgtgacgttc ttcagcacga tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa
    14761 ggccagatga tagtcgctat tctcggaaac ccatgcgctg atatgttctg ctccccagcg
    14821 agtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga gaatgtagag
    14881 aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaatgcacat aagggagtta
    14941 tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga ctgcggcggt
    15001 aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca gattcagcac
    15061 gatggcagta ataccgcccg cggcaatccc ggaggagagc aggtttttca gccattcagg
    15121 ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga ccgaccgcca gcgacagcgc
    15181 gataatcaga atcgcccggc ggttcagcgg ctcacgagaa acgatacgca caccggaggc
    15241 ggcgatggtg ccaaacatta caagcgttgc gccgcccaga accggttctg gaatgtgttg
    15301 tacaaaaccg ctcactgccg ggaacagacc cagcacgatc aacatcagcg cgacgacaaa
    15361 accgacatag cggctggcaa caccggttaa ctggatcacc ccgttgttct gcccgaagca
    15421 ggagttcggg aaggtattaa ataccgccga gacaaacgag ttcaggccgt ttgccagtac
    15481 gccgcctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg aaacgtcaga
    15541 ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca gcatcagcgg
    15601 cagcagcaga ctccattcaa tgccaagacc gtaatagagc ggcgttggca ccataatcag
    15661 ttcttgtgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg ccagcgcata
    15721 tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag taagggttac gttgacggtt
    15781 aagcaggata attaaggcta agaccacgcc tgccagcagc agatttttcg gtgcgccgaa
    15841 ggtgttatcg ctcatggctg cgtaaccgcc gccaatggag gtcagtccaa cctgaattag
    15901 cgacaggccg ataatcatca ccacaacgcc agaaaccagc ggcgtaataa tgcggcgcgc
    15961 cagatgcaga acgcgggaga tcaccatctc ggtacaactt gccagcatca acgtgccgaa
    16021 caaagctgcc atcatggtag gaacatcagc accaccggtc ttcagcgctg tgccgcccat
    16081 aatcagcgga gcaacaaagt tgaagctggt gccctgaata gacaacaacc cggagccaac
    16141 cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca gcgacatact
    16201 aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta atagcgctgg
    16261 cgtgatcacc gcaacgaaca tcgccagcaa atgctgacag gcggcaaaca gagtttgagg
    16321 aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg
    16381 ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg tggtagcaaa
    16441 gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt gccacatcct catctaaata
    16501 tttaaatgag ataaatgtac ttttttatat aacttttagt tgttttctgc ttaaaatcca
    16561 tgccattttt ctattttcag cgaagaattc gaaccaggaa agcatgactc gatactcgtc
    16621 atatttcgcc ttactgcaaa tgcgcgatga agtatgacga gtatgaaaga gtgatgcgga
    16681 cacaaaggag taactatgtt tcatctcgat actttagcaa cgcttgttgc cgcaacgctg
    16741 acgttgctgc tcgggcgcaa gttggtccat tccgtctcct ttttgaagaa atacaccata
    16801 ccggaacctg ttgcgggtgg tttattagtg gcgctggcgc tgctgatact gaaaaaaagc
    16861 atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc cgttgatgct ggctttcttc
    16921 gccaccattg gcctgaacgc caaccttgcc agtctgcgag ctggtgggcg tgtggtgggg
    16981 gttttcctga ttgtggttgt tggcctgttg gtgatgcaaa atgccattgg cattggtatg
    17041 gctagcctgt tagggcttga tccgctgatg gggctgctgg ccggttctat tacgctttcc
    17101 ggcggtcacg gtacgggtgc tgcatggagt aaattgttca ttgaacgtta tggtttcacc
    17161 aacgcgacag aagtggcgat ggcctgtgca acgttcggtt tggtgctggg cggcttgatt
    17221 ggcggtccgg tggcgcgcta tctggtgaaa cactccacca caccgaacgg tattccggat
    17281 gaccaggaag ttccgacggc gttcgaaaag ccggatgtgg gccgcatgat cacctcgctg
    17341 gtgctgattg aaactatcgc gctgattgct atctgcctga cggtggggaa aattgttgcg
    17401 caacttttgg ctggtactgc ttttgaacta ccgaccttcg tctgtgtact atttgttggc
    17461 gtgattctga gcaacggtct gtcaatgatg ggcttttacc gcgtctttga gcgagcggta
    17521 tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat ggggctgaaa
    17581 ctgtgggagc tggcttcgct ggcgttgccg atgctggcga ttctggtggt acagaccatc
    17641 ttcatggcgt tgtacgccat cttcgttacc tggcgcatga tgggcaaaaa ctacgatgcg
    17701 gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac ggcaatcgcc
    17761 aacatgcagg cgatcactga acgctttggc ccgtcgcaca tggcgttcct ggtcgtgccg
    17821 atggtcggtg cgttctttat cgatatcgtc aatgcgctgg tgattaagct gtatttgatg
    17881 ttgccgattt ttgccggtta accgatgaag cggcggtctt ctcaccgccg ctttttacgc
    17941 attcgagtaa cgttctgttt ctggcatcca gcgttctatc agggcttttg cctgttgtgg
    18001 gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg
    18061 cagtaaatcc gccactttaa attcagcatt acccgtctga cgtgtaccta acaattcgcc
    18121 agggccgcga atctccagat ctttctgtgc aatcacaaaa ccatcgttgc tgtcgcgtag
    18181 tacctgcagg cggatttgcg cggttttgga gagcggcgtt ttgtagagca gcacgcagtg
    18241 agaagccacc gcgccacgac ctacacgccc gcgaagctgg tgtaactgcg ccagacccag
    18301 acgctccggg ttttcgataa tcatcagact ggcattgggt acatcaacac cgacttcaat
    18361 gacagtagtg gcaaccagca ggtgtagctc gccttgttta aacgatgtca tcaccgcctg
    18421 tttctcggcg ggtttcatcc gtccgtgtac caggccaaca ttcaactctg gtagcgccag
    18481 tttcaactct tcccaggtcg cttccgccgc ctgcgcttcc agtaattccg actcttcaat
    18541 caacgtacaa acccagtacg cctgacgccc ttcagtcata caggcgtggc gcacgcggtc
    18601 aatgatgtcg gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg
    18661 tggcagctca tctatcaccg aggtatcgag atcggcatac gcagtcatcg ccagcgtgcg
    18721 ggggatgggc gtggcggtca tgatcaactg atgcggatgg aagccctgct gctggccttt
    18781 ctcccataac gccagacgct gatgcacgcc aaaacgatgc tgttcgtcga taatcaccag
    18841 cgccaggccg ttaaactgca cctgttcctg gaaaatggcg tgcgtaccaa caatcatctg
    18901 cacctgaccg ctggcgatgg cttcttgctg agccagtcgt gctttacctt tctgttttcc
    18961 ggcgagccag cctacttcga taccgagcgg tgcaaaccag ttacggaagt tattggcgtg
    19021 ctgctcggcg agtaattcgg ttggtgccat caatgccacc tgtttgccgt gcgcaatcgc
    19081 acgcaacgcg gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgcaccag
    19141 acgcatcatc ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg
    19201 tgcgcccgtt ggcttgaacg gtaaggcggc gaggagttta tttttcagcg cgtcattggc
    19261 gctcagcggc tgagcatgaa aacgttgtgc accggcacgt aacgccaaca tgctgaggtt
    19321 gtgcgccagc aactcttcca gaatcagccg gcgttgtgcc ggatgctgcc cggtttcgag
    19381 atcgctaagc tgtagcgtcg gcggtgggcg gtgcaaagtg cgcaacgctt ccggtagcgt
    19441 catcattcct tgtgacagtt ccggcggcag gagttcttca atggcgcatg tgtcgagcag
    19501 atccagcgcc tggtcggtta atttacgcag cgtagcctgc tttacgcctt ccgtagttgg
    19561 gtaaaccggc gtgagcgttt cctgtaactc tggcgtgctg agatcgccct gtacgcggta
    19621 ttccgggtgg atcatctccg caccatattt gccgcgcttt gcttcgccat aggccagcac
    19681 gcggcggcct gtcgccaggc tatttttcat tgccgcattg aagttgaaaa agcgcatagt
    19741 gagaatgccg gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat
    19801 attgcagttc agcacttcgc cttccaccgt ggcataaaca cccggcagta gttcaccgat
    19861 ggggtagaga tgtgtgcgat cttcgtagcg cagaggaagg tgtaagagca aatcctgtac
    19921 ggtatgcagg ttgattttcg ccagcttgtt actaagcgct gcgccaacgc ccgttaggga
    19981 actgagcggg acagcatcta acaggcgacc tttcatcgcg cttaccctgc ggcctgcatg
    20041 gtggaccacc agtcagcatc ggcttcaatc tcgccttgct gattaacgtg ggggtagggc
    20101 agacctttgc gttttgcgac tttcgccagc accggatagc cgccttcaaa cagcaggcgt
    20161 tgttgctcgg cttccggcaa catgctgttt tcacgcaggt acatgcccgc attttgccgc
    20221 tggcgctggg cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc
    20281 atgccgatca tcggaatgat gatatcctga tccgccaggg ccaacgcttc ctgcgtgatg
    20341 cccgttttct cctgtcccat caaaatacag gtcgggcgag tgtaatcaat ttcgcggaaa
    20401 tcaacagcgt tatcagaaag atgggttgcc agaatctgca tgccctggcc tttcagatga
    20461 gcgacggcat cgccaatggt gcggtgcgtt ttcacctgta cccagctgtt actacccgca
    20521 gctgccgaag ccatggtgcg catccggctg ccaggccaga cagcatgaac ttcatgtacg
    20581 ccaacggcat ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc
    20641 atgcagacgg tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt
    20701 gttgggttca taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg
    20761 cggattttgc gcatgatatt cgccagatgc acacggtcac gggcggtcag acgaataaag
    20821 gcgctataga cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaggtcgtg
    20881 gtgttaattg ccgccgtcag gtttgccagc gcgccctgat gattgaacat ctccaccttg
    20941 atttcggtga tgaattcctg cgccgtctct ttatcccatt caacagccat aaacttctct
    21001 ggctctttct ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta
    21061 ccggggctga cgtgggcgat aatcgggtcg ccaggaattg ggcggcagca tttcgcaaag
    21121 gtgatcagca cgccatcggc acctttaatg ggcagatgtc cgtgactttg ggttgccggt
    21181 ggaatggagg cgtccccatg ttgcagattt ttcgcgacca ccacgctcat tgcgttacca
    21241 aggccgattt ctgccagcag atcgtcaagc gttgccagct tcatgcggtc cagctcgcgc
    21301 tgaatatttt cctgcgggat ttcattgagc ttacggctac cacccaacgc atggttgagc
    21361 agacgacggc ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt
    21421 ttggcgcgcg ctttcgagct gacgacaaag ttcagccacg cggcattcgg gcgagcaccc
    21481 ggtgcggtaa tgatttcaac ggtttgaccg ctagtaagcg gctgcgacag cgggtaaggc
    21541 tggcggtcga cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac cgcataagcg
    21601 aagtcgacgg gcgttgcacc ggcaggcagt tcgacaatgc gcccttctgg tgtgaaaacg
    21661 taaatctcat ccgggaagag atcggattta acgctctcga taaattcaaa cgagctaccg
    21721 gcactctgtt gcagctccag caggctttgc atccagcgct gggcgcgtat ttgcgcggta
    21781 gtgctggttt cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc
    21841 tgatccatat cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc
    21901 gaggtgtgca aagactgata gccgttcgct tttggaatgg cgatatagtc tttcacgcgg
    21961 cccggacgcg gcttgtacag gctgtgcatc tggcccagca cgcgataaca ggtgtcagaa
    22021 tcattgacga tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg
    22081 agcaccattt tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt
    22141 attcccgctt cctgcaaacg cccttcgatt tcagaaagaa tcttctggat catctcttta
    22201 cggttgccgc gcgcggcttt caccacttct ttgattacgc gataacggtt gggatacagc
    22261 gcctcaaaac ccagctcctc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc
    22321 agcgggctgt atatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt
    22381 gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt tgatgagaat gacgcggata
    22441 tcctgcacca tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg
    22501 cggaacttga gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca
    22561 aaaagctgtt ccatgtcctg gtaggtggcg ggtgtatctt caatcacatc atgcagcagc
    22621 gccgccatca gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgccacc
    22681 ggatgcgtga tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca
    22741 acgagatacg cctgccgcag acgcttgatt tggtcttccg gcaggtagtt ttgaatcagc
    22801 tgattcaggc tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca
    22861 gcaatagcgg taacggcttg taattcagcg gcttcctgct cttgctgctc ctggcgttcg
    22921 cgaacgtcga ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca
    22981 gtggttttat cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga
    23041 gcgcgacgcg cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc
    23101 tgaacagtta cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga
    23161 tactgaaatc aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt
    23221 tggcggctca tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta
    23281 tcgaagtcat cattcacaat caggtaatca tattcggcgt aatggctcat ttctgcaaca
    23341 gcttgcgcca tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg
    23401 cggtccagtt cgattttaga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc
    23461 tggcgaattt gctgcgcacc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc
    23521 agtacttgct caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt
    23581 tcgaggaacg catctctact aatcatttct ttaaattcat catgattaac aaagaaataa
    23641 tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg
    23701 gtgtcataca acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca
    23761 ctgggggcag aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta
    23821 gcgaaataaa gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt
    23881 gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc tgcttccgca tttgcctgtg
    23941 attcactgca ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc
    24001 ggctttgcct tcaccacatt gtatatctgc ctgactatct acagcggagg tgatgatgaa
    24061 agtatggatg gcgatattaa taagtatctt gtgctggcaa tcatcggtgt gggcggtctg
    24121 tccggcctgg tcgccagcca gagcacagga agaaatttcc cgcctgcaac agcaaataaa
    24181 acagtgggac gatgactact ggaaggaagg gaaaagtgag gtggaagacg gtgtttacga
    24241 tcagttaagc gcccgtctta cgcagtggca acgctgtttt gggaacgaga cccgcgatgt
    24301 gatgataccg cctttaaacg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa
    24361 aatggcggat aaaaacgcat taagtctttg gatgcgcgaa cgtagcgatc tttgggtgca
    24421 gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc
    24481 aatcagtcgc ggcaatggcc tgaaaggcga ggactggacg cagaaagttc gcttaatttc
    24541 cgctgtgccg caaaccgtta gcgggccttt agccaacagc acgcttcagg gggaaatatt
    24601 tctcaagcgt aaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt
    24661 tgctggcctg atgatgcgcc aggggaatag cgatacgcta aactcactgg ctgtttttgt
    24721 ctgggcatgg ccggatggac cgcatttaat gaccgatcgt ttaaaggatc tggctaccgc
    24781 aggttttact ttaacgcaga cgtatacccg cgcggttaaa aatgctgatg aggttgcgca
    24841 cgtacgcaat gagtggtgga aagcaaaatt acccttcgtc accgatggcg tggttgtgcg
    24901 agcggcgaaa gaacccgaat cccgtcattg gctaccgggc caggcggagt ggctggtggc
    24961 ctggaaatat caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg
    25021 taagagcggt aaaatatcgg tggttgcgtc acttgtacct gtcatgctgg atgataaaaa
    25081 agtccagcgg gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg
    25141 tgatcagatc ctcgtcagcc ttgccggtca ggggattcct cgcattgatg atgtggtctg
    25201 gcgcggagca gaacgtacaa aaccgacacc accagaaaac cgctttaact cgttgacctg
    25261 ctactttgct tctgatgttt gtcaggaaca gttcatttca cgcttagtct ggctggggtc
    25321 aaaacaggtt ctggggcttg atggcattgg tgaggccggc tggcgcgccc tgcatcagac
    25381 tcatcgcttt gagcatatct tttcctggct tttattaacg ccagagcaat tacagaacac
    25441 gccggggatc gcgaaaagta aaagtgcgca gttatggcat cagtttaatc tggctcgtca
    25501 gcagcctttt actcgctggg tgatggcaat gggaataccg ctaactcggg cggcgcttaa
    25561 tgccagtgat gaacggtcct ggtcgcaact tttatttagc acggaacagt tctggcagca
    25621 gctgccgggg actggatcgg ggcgcgccag acaggttatt gaatggaagg aaaatgcgca
    25681 aatcaagaag ctgggcagtt ggctggctgc ccagcagatc acaggttttg aaccttagtg
    25741 accttcgtgg ctgtagtaaa agaccggtaa tcccagctta agacgtagcg ccagtaaacg
    25801 ggcaaaaaag ccgaatacca gggtggagat aatcaccaca tcatggttag aaacatagtg
    25861 ttgcagcgcg atgtacagca cggcggaggc aaatgagaca cccgcataca actctttctg
    25921 gaataccagt gggatgcgtt tacagaacat atcgcgcaaa acgccgccaa acacgccggt
    25981 ggttaccgcc gcgacaacgg caataatcgg gccgtgcccc atatccagag caacctgtgc
    26041 gccgatgata gaaaatacca ccagtccgag cgcatcgagc accagaaaca ctttgcgcag
    26101 ataaggcatt acaggagcaa ctatagtggt gagtactgca gcggtagcga cgataatcac
    26161 atattccggg tgtttgaccc agccgagcgg atagtggccc agcagaatat cgcgcactga
    26221 ccctccgcca attgccgtgg cggtcgcaat aataattacg ccaaatgtat ccatgcgccg
    26281 tcgtccggcc gccagcgccc ccgtcatggc ttcggcagta atgccaacca aatacaaaat
    26341 gtgtaacagc atggttctct ccctaaaaat ctgtctggaa gggtagcgat ttgtgcgcat
    26401 tgtcacgatt gagatttttt aaggagagaa tagattgata atttgtagtt atgtgaaatt
    26461 gctattttct gtagtaagaa tgcatttttc tgattttgtg ataagtaaaa tgcattagaa
    26521 aaagtatgga gtaaataaat atgaggacga aactatatgc gtagcgccgc tgtaattaac
    26581 aacggcctac ggagatatgt cgcttactcg atattctgaa tctgctcgcg catctgttca
    26641 atcaacactt tcagctcgat ggcggagttt gtcacttcgg cgttgataga tttcgacgca
    26701 agagtgttcg actcgcggtt gaactcctgc atcataaagt ccagacgacg accaacggct
    26761 tcttttttct tcagaatgtt gtaggtctct ttgacatgcg cttcgaggcg gtccagttct
    26821 tcggcaacgt caattcgttg tgccagcaga accaactcct gctccagacg gttgttttcc
    26881 agttgcacct gagcatcttc cagcttcgct accagacgct cacgctgcca ttgcaggatt
    26941 tccggcatat gggcgcggac tttgaccact tcggcggtga cgccttccag acgctgctca
    27001 atcaacactt tcagcgcctg accttcggtt tcgcgcgcga tgataaagtc atccagggtg
    27061 ccatccaacg ccgcgagaat ttcagctgca atcgcgtcga gatcttgctc ctgggctgcc
    27121 atcacgcccg gccagcgtag aatatcaacc gggttgattt ctccttcgtc gctctgcatt
    27181 tttacccagt tcgcggcagt taccagctgt ttagccagtt tttcgttgag gatcagctca
    27241 ccttgcgcgc taacatctgg ctcatagcgc agagtacatt ccactttacc gcgcgtcagg
    27301 cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac ggaactgctc cggcagacga
    27361 aagtaagttt ccagataacg ctggtttacc gagcgcattt cccaggttgc gctcccccat
    27421 tcacccttga tttcacgccg ggcgtaggcg gtcatactgc ggatcataga cgttcctgtt
    27481 tataaaagga gaggtggaag gattatagcc atcgatgcct tgtcaggata ggaataaccg
    27541 ccggaagtcc gtataatgcg cagccacatt tgtttcaagc cggagatttc aatatgcgtc
    27601 cagcaggccg tagcaataat caggtgcgtc ccgttaccct gactcgtaac tatacaaaac
    27661 atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa agtattgtgt accgcctcta
    27721 ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc accgcagagt
    27781 acggtatgct gccgcgttct acccataccc gtaacgcgcg tgaagcggcg aaaggtaagc
    27841 agggtggacg cacaatggaa atccagcgtc taatcgcccg tgctcttcgc gcggcagtag
    27901 atttgaaagc actgggtgag ttcaccatta cgctggactg tgacgtgctt caggctgatg
    27961 gtggcacgcg taccgcttcg attacgggtg cctgcgtggc gctggcagat gcgctacaga
    28021 agctggtgga aaacggcaag ctgaaaacta atccaatgaa agggatggta gccgcggtct
    28081 ctgtcgggat tgtgaacggt gaagcggttt gcgatctgga atatgttgaa gactctgccg
    28141 cagagaccga catgaacgta gtgatgaccg aagacgggcg tatcattgaa gtgcagggga
    28201 cggcagaagg cgaaccgttc acccacgaag agttactcac cttgttggcg ctggcccgag
    28261 ggggaatcga atccattgta gcgacgcaga aggcggcgct ggcaaactga tttttaaggc
    28321 gactgatgag tcgccttttt tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa
    28381 accatatcag cgccagttta ttgaatttgc gcttagcaag caggtgttaa agtttggcga
    28441 gtttacgctg aaatccgggc gcaaaagccc ctatttcttc aacgccgggc tgtttaatac
    28501 cgggcgcgat ctggcactgt taggccgttt ttacgctgaa gcattggtgg attccggcat
    28561 tgagttcgat ctgctgtttg gccctgctta caaagggatc ccgattgcca ccactaccgc
    28621 cgtggcgctg gcggagcatc atgaccttga cctaccgtac tgctttaacc gcaaagaggc
    28681 aaaagaccac ggtgaaggcg gtaatctggt tggtagcgcg ttacaaggac gcgtaatgct
    28741 ggtagatgat gtgatcaccg ccggaacggc gattcgcgag tcgatggaaa ttattcaggc
    28801 caatggcgcg acgcttgcag gcgtgttgat ttcgcttgat cgtcaggaac gcgggcgcgg
    28861 cgaaatttcg gcgattcagg aagttgagcg tgattacaac tgcaaggtga tctctatcat
    28921 caccctgaaa gacctgattg cttacctgga agagaagcca gaaatggcgg aacatctggc
    28981 ggcggttaag gcctatcgcg aagagtttgg cgtttaacga aactcgccgg atgaaaagtc
    29041 atccggcgtc atattactgc aactgtgcgg caattagcgg ccagcgggcg tcaaaatcat
    29101 ccgtcgggcg gtatttaaac tcgctgcgga caaatcgtga cagcatacct tcacaaaatg
    29161 ccagcaactg gcttgccagc agggtttcat cggtggtgta accttcaccc tcacgcattc
    29221 tcttttcacg caatacctga cgcaactgcg cttcaatacg ctcgaacagc tggttgatgc
    29281 gcccttgcag acgatcctgt tcgaacatta gcgcatgacc agtgaggatg cgagtcaggc
    29341 caggattacg ctcaccaaaa ccgaggagca acaacacgat cagacgcagg cgcgctgtgg
    29401 tgtctttctc atctttcaga atcaggttga tacgagtaat caggctatct tcgataaact
    29461 caatcaggct atcgaacatg cgggtcttac tggggaagtg gcgatacagt gccgcttcgg
    29521 aaacgccgac agaggcggcc agttttgccg tcgtgatacg ttggcttcca tcgctggatt
    29581 ccagcatcag cgccagagac tgaagtattt cctcgcgacg gttccttttc gcagtttgtt
    29641 tttctgccat gttacaaaat acccctgaaa ataagcactt gccaggcggc acccacgcta
    29701 tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg tatgtgttac
    29761 tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc
    29821 agattaaatt cagcctgtac taccggaaca aaaatcatct gggcgatgcg ttcgccaggt
    29881 tgaatggtga agctgtcctg accacggttc cacacggaaa tcatcaactg gccctgatag
    29941 tcagaatcga tcaatcctac caggttacca agcacgatac cgtgcttatg tcccaatccg
    30001 gagcgcggta gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc
    30061 ggaaccagcg tagtgtcacc cggagccagt tctacggcgt cgtcgagaca ggcacgcagg
    30121 tcaagtccgg cagagccaga ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc
    30181 gggtccagaa tcttaacgtc gatttttttc atcataacgg gtcacgatct cgtcgagtaa
    30241 taattggcca aggagctctt tgcgctcaag cggtaagact ttatctccgt cctgccagaa
    30301 aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc
    30361 gcagatcaga tcaaggtttt tacggatacg tttttgccgg gcgtattctt ccacattatt
    30421 tgtttcggcg gcaaatccaa cgacgtaagg tcgatggtct tttagtgcgg caacgcctgc
    30481 gacgatatcg gggtttttaa ccatttttat tgttaattca tcaccctgcg tggcctgctt
    30541 tttgattttt tctggggcca cggtagctgc gcgataatcc gccacggcgg cgcaaccgat
    30601 aaaaatattt tgctgctgta ctgaagcatt cacggcggct tccatttcca gcgcggtcat
    30661 cacatcaaca cggttaacaa acggtggcgt cggtagtgaa accggacctg ataccagcgt
    30721 gacgttcgcg ccacgacggg ctgcggcggc ggcgatagca aaacccatct tgccggagct
    30781 gtgattagag atataacgca cgggatcgag cggttcacgc gtcggaccgg cggtaatcat
    30841 aatgttcaga tgtttcaggt cgttgacggg cgaaaaatgc gctaccgcca tatccacaat
    30901 agttaacgga tcgagcattc gtcctgggcc gatatcacca caagcctgac tgccgctgtc
    30961 tggcccccag atgagcaaac cacgtgaggc aagcacctct aaattatgct gggtggcggc
    31021 ggcacggtac atctgctggt tcatggcggg gagcacggct acaggcgcag gtgtagccag
    31081 acaaatcgtc gataccaggt cattcgccat tccggctgca acacgggcaa ttaaatctgc
    31141 cgtggcaggg gcgagaatca ctaaatcagc ccattttccc agctcaatat ggcccatagc
    31201 ggcttcggct gccgggtcca gcagactgtc ggaaacggga taaccagaaa ccgcctgcaa
    31261 gctaagtggg gtgataaagg cttttgccgc ttcggtcatg gctacgcaga catcggcccc
    31321 gcgatcgcgc aaacgacgca ccagttcagg ggttttatag gcagcaatac cgccgctaac
    31381 gccgagaacg atttttttac cggccaggct catgatgatt tttcctgttg ggtgacacca
    31441 gaagttggcg attttatcac aatacttttg ttgtcgtgcc ttcacctgag attcactttg
    31501 cgaggcgctt tccaggattg aaaactggcc gtcgatttaa aggaacggct atgacaggat
    31561 gcgagcacca caaaggaggt gaaggtgaaa aacaatgtac agctgttgat gccgcgcgaa
    31621 aaaatgctga agtttggtat tagcgcctta acggatgtcg agctgctggc gctatttctg
    31681 cgtaccggaa cgcgcggtaa agatgtatta accctggcaa aagagatgct ggagaatttc
    31741 ggctctcttt atggcttgtt aacctctgaa tatgagcaat ttagtggcgt tcatggaatt
    31801 ggcgtggcga aattcgccca gttaaagggg attgccgaac tggcgcggcg ttactacaac
    31861 gtacggatgc gtgaagaaag ccctttactc agcccggaga tgacgaggga atttttacaa
    31921 agccagctca cgggcgagga gcgggagatc tttatggtga tctttctcga ctcccaacac
    31981 agggttataa cgcatagccg tcttttttcc ggcacgctaa accatgttga agtccatcct
    32041 cgggaaatta tccgcgaagc gataaaaata aacgcctcgg cgctgatcct tgcgcataat
    32101 cacccttcgg gttgtgctga acccagtaaa gcggataaac tcattactga acggataata
    32161 aagagttgtc agttcatgga tttacgcgtg ctcgaccata tcgtgattgg gcgtggagag
    32221 tatgtttctt ttgccgaacg cggctggatt taacccgcta tgcgcgatcc ttcgggatct
    32281 ttgtctgttc gggacttgag cacatcgctg agtcagcgta tactacgcca cctttgagaa
    32341 tctcgggttt ggcatttggg cctggcaatc gagagttcac atagaactgc gatgaccggg
    32401 ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct cgagctaatt tgatttttgg
    32461 agaatagaca tgtcccgagt ctgccaagtt actggcaagc gtccggtgac cggtaacaac
    32521 cgttcccacg cactgaacgc gactaaacgc cgtttcctgc cgaacctgca ctctcaccgt
    32581 ttctgggttg agagcgagaa gcgttttgtc accctgcgcg tatctgctaa aggtatgcgt
    32641 gtaatcgata aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg tggcgaaaag
    32701 tactaagtac ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa atcaagctgg
    32761 tttcttctgc tggtactggt cacttctata ccactacgaa gaacaaacgt actaagccgg
    32821 aaaaactgga actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc tacaaagaag
    32881 cgaaaatcaa ataatttccg ctttgatgta acaaaaaacc tcgccccggc ggggtttttt
    32941 gttatctgct tgcccccata ttgactgcat ctgttcattc ctggagatgc tatgcctgaa
    33001 ttacccgaag ttgaaaccag ccgtcgcggc atagaaccgc atctcgttgg tgcaaccatt
    33061 cttcatgcgg tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga gatctaccgt
    33121 ttaagcgacc aaccagtgct tagcgtgcag cgccgggcta aatatctgct gctggagctg
    33181 cctgagggct ggattatcat tcatttgggg atgtctggca gcctgcgcat ccttccagaa
    33241 gaacttcccc ctgaaaagca tgaccatgtg gatttggtga tgagcaacgg caaagtgctg
    33301 cgctacaccg atccgcgccg ctttggtgcc tggctatgga ccaaagagct ggaagggcac
    33361 aacgtgctgg cccatcttgg accggagccg cttagtgacg atttcaacgg tgagtacctg
    33421 catcagaagt gcgcgaagaa aaaaacggcg attaaaccgt ggctgatgga taacaagctg
    33481 gtggtaggcg tagggaatat ttatgccagc gaatcgctgt ttgcggcggg gatccatccg
    33541 gatcggctgg cgtcatcact gtcgctggcg gagtgtgaat tgttagctcg ggtgattaaa
    33601 gcggtgttgc tgcgttcgat tgagcagggc ggtacaacgc tgaaagattt tctgcaaagt
    33661 gatggtaaac cgggctattt cgctcaggaa ttgcaggttt acgggcgaaa aggtgagccg
    33721 tgtcgggtgt gcggtacgcc gattgtggcg actaaacatg cgcagcgggc aacgttttat
    33781 tgtcggcagt gccagaagta attgatgcgc gccggatggc ataccatccg gcataaactc
    33841 tacgctaact tcgccatcag cgcctgatgg acattctccg gcaggaaatg ggtgacatcg
    33901 ccctgatggc gcgccacctc tttcaccaac gatgaagaga taaacgacca ctctttcgac
    33961 ggcatcagaa acacactttc cagctccggc attaagtggc gattcatatg cgccagctgc
    34021 atttcatatt caaaatccgc caccgcacgc aggccacgaa tcagcaccgt agcgtgttga
    34081 ttacgggcga agttcgccat caaatcacta aacccgacca cttccacgtt ctccagatgc
    34141 gcggttgcct gctgtgccag ctccacacgc tcttccaggg taaacatcgg ttttttactg
    34201 ggactagcgg caatcgccag aataacgtga tcgaacattt gcgaggcgcg cgtcacgata
    34261 tcgatatgac cattggtaat gggatcgaaa gtacccggat aaatcgcccg tttttgcata
    34321 acaacctcaa tgcgttttcg gtggcaggta aggttccagc agttgaagca gacgctgtag
    34381 tgcgccctgg ttttgataca gcacttcaac ggcatgacgg ccatagaaac tacggtaatc
    34441 ggcgtcggtg agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg taacggtaat
    34501 cagcccgctt gcctgctcca gccgcgcgca aatgtcttta aagttaaacg tatgcggccc
    34561 catcaatacc ggaatagcgt gtgcggccgc ttccagcgga ttatgcccac cacgttcaac
    34621 cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac agtaacatca actcgcccat
    34681 cgtatcgcca accacaacct gcgtgctggt ggaggggact tcccctgaag atcgtgtgat
    34741 atagcttagt ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt ctggatgacg
    34801 gggtaccagg atgagcaata aattcgggaa ttgctgtaac aatgcctgat gtgcggcgat
    34861 caccacactc tcttcgcctt catgagtgct ggtggcaatc cataccgggc ggtgtggtgc
    34921 ccactggcgg cgcagcgtca cggctttagc agccaactgc ggcgttacag aaatatcgaa
    34981 tttcaggcta ccggtaacgg tcacctgatt attttttgcg cccagcgcca caaaacgtgc
    35041 accatcttct tcattttggg cggcaatcag cgtaatacga cgcagcaagc ggcggacgaa
    35101 tttacccagt ttggcataac ctgcggccga gcgggcagag agtcgcgcgt tagcgatcac
    35161 cagcggaatt ttacgtttat gtagcgccgc aatcaggtta ggccatagtt cggtttccat
    35221 aatcaacacc agtttagggt cgactttatt caggaaacgg ttgagtgcat cgggcaggtc
    35281 atacggcaga taaacgtgct gaacatcctt cccgaaagcc gattgtacgc gctccgaacc
    35341 ggttggagtc atggttgtta cggtaatcgg taaatcagga taacgatgac gcagtgcgcg
    35401 caccaacggg atcgccgcca gagtttcacc gacggagacg gagtgcagca taatgccgcc
    35461 tggttttagc ggatggcggt aaaaaccgta acgttcaccc cagcgttttc gataggccgg
    35521 agccttacgt ccgcgcaccc agagccgtat ccagatcagt ggctgaataa ggtagagaag
    35581 ggcggtgtaa agcaattcga gcatagtaaa tagctgactt atggatgtgc tggggattct
    35641 atgtatttag ctgtggcttt accattactt ttcccgtttt tgacttaaat agcaatttac
    35701 ccgccaattc ttgctgaaca tctatacttt ttttgtattt ttatgcgatt cattgatttc
    35761 tgcagtacgt gcaggaagga atcgcagagc aacagtaaaa agaatatggt tgctaatgat
    35821 tttatttgat tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca ttcgggtcgg
    35881 attgccgcga aagaagtgat acactagcgc gtcaatatca gtgcggtatc agtattcagg
    35941 tagctgttga gcctggggcg gtagcgtgct ttttctgctt caccttaatc ggataatctc
    36001 aacaaaaaga gttacttgtg gataagccat ttcgaagaat tttgctcatt aagatgcgtt
    36061 ttcatgggga tatgttatta actactcccg tcattagttc gctgaaaaaa aattaccctg
    36121 acgcaaaaat cgatgtgctg ctttatcagg acaccatccc gatcctgtct gaaaatccag
    36181 agattaacgc gctctacggc ataaaaaata aaaaagcaaa agcctcagaa aaaattgcca
    36241 acttttttca tctcatcaag gtattacgtg ccaataagta tgaccttatc gttaatctta
    36301 ccgatcaatg gatggttgct atactggttc gcttattaaa tgcccgtgtg aaaatttccc
    36361 aggattatca tcatcggcag tctgcttttt ggcgtaatag tttcactcat ttggtgccgt
    36421 tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct gacgccattg ggacttgaat
    36481 cgttggtgaa gcagacaacc atgagttacc cgcctgcaaa ctggaaacgt atgcgtcgcg
    36541 aacttgatca cgctggtgtt ggacaaaatt atgtggttat ccaacctacg gcgcggcaaa
    36601 tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat tgatgcctta catgctcgtg
    36661 gttatgaagt cgttctgacg tccggcccag ataaagacga tctggcctgc gtcaatgaaa
    36721 ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc cggaaaggtg accttcccgg
    36781 aacttggtgc gttaatcgat catgcgcagc tgtttattgg cgttgattcc gcaccggcgc
    36841 atattgccgc tgcagttaat acgccgctga tatcgctgtt tggtgcgacg gaccatattt
    36901 tctggcgtcc ctggtcaaat aacatgattc aattctgggc gggagattac cgggaaatgc
    36961 caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc ggttattccg gcggcagatg
    37021 tcattgcagc tgtcgataaa ttacttccct cctccacgac aggtacgtcg ttatgatcgt
    37081 tgctttttgt ttatataaat attttccctt tggcggtttg cagcgcgatt ttatgcgtat
    37141 tgctcagaca gtcgccgccc gaggccatca tgttcgggtt tatacccagt cgtgggaagg
    37201 cgaatgccct gatgtatttg aactgatcaa agtgccggtt aaatcgcata ccaatcacgg
    37261 gcgcaatgcg gaatattttg cctgggtgca aaaacattta cgtgaacacc ccgtcgatag
    37321 agttgttgga tttaataaaa tgccggggct ggacgtttat tatgccgctg atgtttgtta
    37381 tgccgagaaa gtagcgcagg agaaaggctt tttctatcgc ctgacgtcac gttatcgcca
    37441 ttatgctgcc tttgagcggg caaccttcga acagggcaag ccgacacagc tgctgatgct
    37501 gacagataag caaatcgccg atttccagaa acattatcag actgaagcgg aacgttttca
    37561 tattctgcca ccggggattt atcctgatcg taaatatagc cagcagccag ccaatagccg
    37621 tgaaatcttc cgtaagaaga atggaataac cgaacaacaa tatttattgt tgcaggttgg
    37681 ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt cgttaccgga
    37741 ttcgctgcgc cacaacacat tgctatatgt tgttgggcag gataaaccgc gaaaatttga
    37801 ggcgctggca gaaaaacgcg gcgtgcgcag taatgttcac ttcttctcgg ggcgcaacga
    37861 tgtctcggaa ttaatggcgg cggcggattt attacttcat cctgcctacc aggaagccgc
    37921 gggaattgta ctgctggaag cgataactgc aggattaccg gtactaacaa cagccgtttg
    37981 tggctatgcg cattatattg ttgacgccaa ttgcggcgag gctattgctg agccattccg
    38041 ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg caatcttcat tgcgccaggc
    38101 ctgggcggaa aatgcgcgac attatgctga tacacaagat ttatacagtc tgccagagaa
    38161 agcggcggac atcataacgg gtggtctgga tggttgaact taaagagccg tttgccacgt
    38221 tatggcgcgg taaagatcct tttgaggaag ttaaaacctt gcagggtgag gtatttcgtg
    38281 aactggaaac tcgccgcact ctgcgctttg aaatggcggg caaaagctat tttctcaaat
    38341 ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt actctcattg cggatgccag
    38401 tattaggcgc agaccgcgaa tggaatgcga ttcatcgact gcgggatgtc ggcgttgata
    38461 ctatgtatgg ggtggcattc ggcgaaaaag gcattaatcc gctgaccaga acctcgttta
    38521 ttattaccga agatctgaca ccaaccatca gtctggaaga ttactgtgct gactgggcga
    38581 ccaacccacc agatgttcgc gtgaagcgta tgcttattaa gcgtgtcgcg acgatggtgc
    38641 gcgatatgca tgctgcgggc attaaccacc gtgactgtta tatctgtcat ttcctgctgc
    38701 acttgccttt ttccggtaag gaagaggagt taaaaatttc agtaattgac ctgcaccggg
    38761 cgcagatacg tgcaaaagta ccgcgtcgtt ggcgggataa agatttgatt ggtttgtatt
    38821 tttcatcaat gaatattggg ctgacgcaga gagatatctg gcgatttatg aaggtttatt
    38881 ttgggatgcc tttacgtaag atattatctt ttgaacggaa tttattgaac atggcaagtg
    38941 ttaaggccga gcatattaaa ga
//
LOCUS       DAPQZD010000026        38568 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPQZD010000026 DAPQZD010000000
VERSION     DAPQZD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.026"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(84..1748)
                     /gene="glnS"
                     /locus_tag="RO454_001326"
     CDS             complement(84..1748)
                     /gene="glnS"
                     /locus_tag="RO454_001326"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDY0933190.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1968..2729)
                     /locus_tag="RO454_001327"
     CDS             complement(1968..2729)
                     /locus_tag="RO454_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0933191.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2731..3717)
                     /locus_tag="RO454_001328"
     CDS             complement(2731..3717)
                     /locus_tag="RO454_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HDY0933192.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3710..4945)
                     /locus_tag="RO454_001329"
     CDS             complement(3710..4945)
                     /locus_tag="RO454_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDY0933193.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            5115..6272
                     /locus_tag="RO454_001330"
     CDS             5115..6272
                     /locus_tag="RO454_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDY0933194.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6276..7163
                     /locus_tag="RO454_001331"
     CDS             6276..7163
                     /locus_tag="RO454_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDY0933195.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7223..7981
                     /locus_tag="RO454_001332"
     CDS             7223..7981
                     /locus_tag="RO454_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDY0933196.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            8017..9186
                     /locus_tag="RO454_001333"
     CDS             8017..9186
                     /locus_tag="RO454_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDY0933197.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9183..9653
                     /locus_tag="RO454_001334"
     CDS             9183..9653
                     /locus_tag="RO454_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDY0933198.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9667..10947
                     /locus_tag="RO454_001335"
     CDS             9667..10947
                     /locus_tag="RO454_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDY0933199.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(11036..12982)
                     /gene="nagE"
                     /locus_tag="RO454_001336"
     CDS             complement(11036..12982)
                     /gene="nagE"
                     /locus_tag="RO454_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDY0933200.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13315..14115
                     /gene="nagB"
                     /locus_tag="RO454_001337"
     CDS             13315..14115
                     /gene="nagB"
                     /locus_tag="RO454_001337"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDY0933201.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            14175..15323
                     /gene="nagA"
                     /locus_tag="RO454_001338"
     CDS             14175..15323
                     /gene="nagA"
                     /locus_tag="RO454_001338"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDY0933202.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15332..16552
                     /gene="nagC"
                     /locus_tag="RO454_001339"
                     /gene_synonym="nagR"
     CDS             15332..16552
                     /gene="nagC"
                     /locus_tag="RO454_001339"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDY0933203.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16600..17352
                     /gene="nagD"
                     /locus_tag="RO454_001340"
     CDS             16600..17352
                     /gene="nagD"
                     /locus_tag="RO454_001340"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDY0933204.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17608..19272
                     /gene="asnB"
                     /locus_tag="RO454_001341"
     CDS             17608..19272
                     /gene="asnB"
                     /locus_tag="RO454_001341"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDY0933205.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(19314..19751)
                     /locus_tag="RO454_001342"
     CDS             complement(19314..19751)
                     /locus_tag="RO454_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933206.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            20085..20161
                     /locus_tag="RO454_001343"
     tRNA            20085..20161
                     /locus_tag="RO454_001343"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20119..20121,aa:Met,seq:cat)
     gene            20172..20256
                     /locus_tag="RO454_001344"
     tRNA            20172..20256
                     /locus_tag="RO454_001344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20206..20208,aa:Leu,seq:tag)
     gene            20280..20354
                     /locus_tag="RO454_001345"
     tRNA            20280..20354
                     /locus_tag="RO454_001345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20312..20314,aa:Gln,seq:ttg)
     gene            20389..20463
                     /locus_tag="RO454_001346"
     tRNA            20389..20463
                     /locus_tag="RO454_001346"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20421..20423,aa:Gln,seq:ttg)
     gene            20479..20555
                     /locus_tag="RO454_001347"
     tRNA            20479..20555
                     /locus_tag="RO454_001347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20513..20515,aa:Met,seq:cat)
     gene            20604..20678
                     /locus_tag="RO454_001348"
     tRNA            20604..20678
                     /locus_tag="RO454_001348"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20636..20638,aa:Gln,seq:ctg)
     gene            20716..20790
                     /locus_tag="RO454_001349"
     tRNA            20716..20790
                     /locus_tag="RO454_001349"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20748..20750,aa:Gln,seq:ctg)
     gene            complement(20945..22120)
                     /gene="ubiF"
                     /locus_tag="RO454_001350"
     CDS             complement(20945..22120)
                     /gene="ubiF"
                     /locus_tag="RO454_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDY0933207.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            22266..23690
                     /gene="miaB"
                     /locus_tag="RO454_001351"
     CDS             22266..23690
                     /gene="miaB"
                     /locus_tag="RO454_001351"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDY0933208.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            23843..24883
                     /locus_tag="RO454_001352"
     CDS             23843..24883
                     /locus_tag="RO454_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDY0933209.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            24880..25347
                     /gene="ybeY"
                     /locus_tag="RO454_001353"
     CDS             24880..25347
                     /gene="ybeY"
                     /locus_tag="RO454_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDY0933210.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            25437..26315
                     /gene="corC"
                     /locus_tag="RO454_001354"
     CDS             25437..26315
                     /gene="corC"
                     /locus_tag="RO454_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDY0933211.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            26340..27878
                     /gene="lnt"
                     /locus_tag="RO454_001355"
     CDS             26340..27878
                     /gene="lnt"
                     /locus_tag="RO454_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDY0933212.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSTAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYSRTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(28005..29882)
                     /locus_tag="RO454_001356"
     CDS             complement(28005..29882)
                     /locus_tag="RO454_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDY0933213.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            30336..31244
                     /gene="gltI"
                     /locus_tag="RO454_001357"
     CDS             30336..31244
                     /gene="gltI"
                     /locus_tag="RO454_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDY0933214.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31414..32154
                     /gene="gltJ"
                     /locus_tag="RO454_001358"
     CDS             31414..32154
                     /gene="gltJ"
                     /locus_tag="RO454_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDY0933215.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32154..32828
                     /gene="gltK"
                     /locus_tag="RO454_001359"
     CDS             32154..32828
                     /gene="gltK"
                     /locus_tag="RO454_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDY0933216.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32828..33553
                     /gene="gltL"
                     /locus_tag="RO454_001360"
     CDS             32828..33553
                     /gene="gltL"
                     /locus_tag="RO454_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDY0933217.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            33671..34606
                     /gene="rihA"
                     /locus_tag="RO454_001361"
     CDS             33671..34606
                     /gene="rihA"
                     /locus_tag="RO454_001361"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDY0933218.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            34690..36360
                     /gene="hscC"
                     /locus_tag="RO454_001362"
     CDS             34690..36360
                     /gene="hscC"
                     /locus_tag="RO454_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDY0933219.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(36420..37871)
                     /gene="djlC"
                     /locus_tag="RO454_001363"
     CDS             complement(36420..37871)
                     /gene="djlC"
                     /locus_tag="RO454_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDY0933220.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37868..>38568)
                     /locus_tag="RO454_001364"
     CDS             complement(37868..>38568)
                     /locus_tag="RO454_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDY0933221.1"
                     /translation="EEQYLLFALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGI
                     NNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQT
                     AMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMHYFS
                     AYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTV
                     QPECPVTLKDVSDL"
BASE COUNT         9375 a        10087 c         9718 g         9388 t
ORIGIN      
        1 aaacgccgca gagcgcggcg tttgggataa tttgcatatt tcttgccgga tgcggcgtaa
       61 acgctctatc cgacaacgta aacttactcg cctactttcg cccaggtatc acgcagccca
      121 acggtgcggt taaataccgg tttttccgcc gtagaatggc ggctgtcgag gcagaagtaa
      181 ccttcacgct caaactggaa tgctttaccc gcaaccgcat ctttcagcga cggttcagca
      241 aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca
      301 ccagggttcg gcacgctgaa cagacggtca tacaaacgga tttcaaccgg cagcgcatgt
      361 gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg atctttgctt
      421 aaggtgtcgg cgtcataagt acagaagatg gtggtgatat tgccttcggc atctttctcc
      481 acgcgttctg ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga
      541 cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg
      601 ctaaatggca cctgacggct gcccatttcc ggtttattcg gatggttcgg catggtaacc
      661 atttcgcctt cgccctggta gttttcgata accagtttca ccggatcgat aaccgccatt
      721 gcgcgcggcg cattttcgtt gagatcttca cggatgcagg attccagtga cgccatctca
      781 atggtgttgt cctgcttggt cacaccaatg cgtttgcaga actcacgaat agaagccgca
      841 gtgtaaccac gacgacgcag accagaaatg gtcggcatac gcgggtcatc ccagccttca
      901 acgtgcttgt cggtcaccag caggttcagc ttacgcttgg acatcacagt gtattccaga
      961 ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt gtccagtacc
     1021 cagtcgtaca gacgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct
     1081 tccagcgcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg
     1141 ccagtctggt ggtgttcagc aaacttaatg cggtacagca ccggatcgcg catcacgata
     1201 aacggcgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccgccg
     1261 gtacgcattt tctcgaacag cgccaggttc tcttcaacgc tgcgatcgcg gtacgggctg
     1321 tttttacccg gctgcgtcaa ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca
     1381 tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa
     1441 taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt
     1501 ttgatcgaat cgacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg
     1561 ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg
     1621 ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt ggtgtgctta
     1681 ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg ggcttctgcc
     1741 tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc
     1801 gggacaggct gacaactgtt agttagcgaa aaaatggttt tgcgtaagat attggggcgt
     1861 tttctgcgct actgccagca gatcctcgcg ctggaaacgt aaggccaaac gtcgccagcc
     1921 tgacgcattt attaaatttc cggatgagct ggccgcagag tcgtagatta gtcggctatg
     1981 attaaacgcg tcgacaagga cgcaagcgtt ctacgcgtct gcccatcaag ctgactatcc
     2041 gtaataatct ctgtcagttc attaagatgc gcgatattaa acagcgacca tgaaccgtat
     2101 tttgagctgt cagccaggag aattttgcgc gaagcatgcg cgataagatc gcgttttagt
     2161 gcggctttct cttccgtggg tgctgtaata cccttttcaa gatcccagct gttacagctt
     2221 aaaaatgcca cgtccggcca ggtattttgc agcaatttac gaccatgttc gccaatgcag
     2281 gactggctgg agtcgtcaat acgtccgccg ataatcgtca cttcaatttg cttgaattca
     2341 gccaaaaaaa gcgcgatatg caggtcgctt gtgatcacgc gaagcggtaa atgtgtaatc
     2401 tgccgcgcaa gttcaatcat tgtcgtaccg gcatcaagga cgacggaatc ccccgctttg
     2461 accattgacg ccgctgcgct ggcgatggca tgtttttccg ccaggctgcg ctgcattttc
     2521 tcatgggtag tcggttgggt aggaataaac cggttgagag tcacaccgcc gtgtgtacgg
     2581 ctgataacac cctgctcatc gagttttatc aggtctcgac gaatcgtagc cggcgacgca
     2641 ttggttaccg ccaccagctc atctacggtg accagattat ggcttttcag gtaatccatg
     2701 atctgctcta accggttata tcccttcatt ttattctcgc gaaatctgca ttgctaactg
     2761 gatggaaact gccatgctct cagacttggc ttttcctgtc caggcaatat caaacgcggt
     2821 gccgtggtcg gcagaagtgc gaataaatgg caacccggcg gtgatattca ccccatcata
     2881 aaatccaagc agttttaaag ggatatgccc ctgatcgtgg tacatagcca ctaccatatc
     2941 gaatagccct tcatggcatt gcataaacac ggtatctggc gggcacgggc cagtaacatc
     3001 aatatcttcg gcctgcattg cttcaatcgc aggcgcgata atctcgattt cctcagtgcc
     3061 gaataaacca tgttcccctg catgtggatt tacacccgcc acggcaatac gcggacgctc
     3121 aataccaaca cgtttcagga agtggttcgc aactcgaata accgttttga ccctttcacc
     3181 attcaaagta tccaggaatt tacgcagtga gatgtgagtc gaaatatgga taaccttgag
     3241 cttctcggta tagagcacca tcgcgtactc tttgctgccg gtaagatggg ccagcaattc
     3301 cgtatgcccc ggatagttat gcccgccaag atgcagcgct tctttattca gcggcgcagt
     3361 ggctatggct ttcacctcgc ctgataaggc cagagccgtt gcacgtttga tacagcgata
     3421 cgctaaatcg cctgccacag cctgtaccac tccgggtgtc agcgcatcgg ggttagccag
     3481 cggctcatcc atcacgttaa tcacgccggg agcaaaatgg gcgtcgcaca ccttttgaat
     3541 gacgcgtaac tctgcctgcg gcgtgatatt catcgccata atacgtcgca gcgtgcgcac
     3601 acagcccacc accaccaccg gggcaccagc aagcccggct tcgctcaggg atttaataat
     3661 gatctccggg ccaatacccg ccggatcgcc catcgttacc gcaataatat tattcactac
     3721 gcttctcctc gataaaattc agaacctgat gcaacgtggt ctcatcgcca aacccaccgg
     3781 ctttcgtcat caccgaacgg gaccagacgc cgcccagaaa gtgaccgtat ggaacgcatt
     3841 gcgccacctt ccccgtaata cgaaatccgg tcgcgcctaa cgcactggct gttgccattg
     3901 cgacatcacc accagagaga taaagcgcat cgggcattgc gcaaagcaga acttgccggg
     3961 taagttcgcc aagaaaccgg caaatttttt ccccaagtgc agcgcgactc atctgccaac
     4021 gctggcaaag ggtgtcgatc tgatgccttg ctacagaatc gttacaggtg tggacaatac
     4081 agtggtcgcc tttctgcaag gcctgcacaa tacgcgcgtc atagtcactg gcattgcctg
     4141 ccagaatgtg ttcaacatcg acgtatatct gtgtgatacg tgggtgtgaa tgaagtgtgg
     4201 cgatctgccg ttgagcgatt tcgctcattg aaccaataat ggccagcacg gtgggtggcg
     4261 gcataatcgc ggagcgccgg gcaacggcat cacaaatacc agcggagccg accagcagca
     4321 gcggttgttt agcctgcatc acagcggcag cgatgcgatc caggtcgctg tccgtctgcg
     4381 catctatcac gccaatccac ggttgctcat aagacaaatg gctgagctga gaaaccgtaa
     4441 cgggctggca tggaatggcc gtctgcaacc ggatgatgtc cgcaattcgg gcgctcaata
     4501 ccggggtttt cggatcgcta gcaaactcgg tttccgttat ccgcacaccg tttacccaac
     4561 actcaccggc cactgtggtc cttcccgcct gaggaaatgc gggggcaacc actgcgcccg
     4621 ttataccgca tgccttcatc agggcttcaa tttctgcgcc gatattgcct cgcagcgtgg
     4681 aatctatctt tttaatcacg agcggcgggt tattcgccag agggagatga ctcatcagcg
     4741 aggttatttt catcgccgcc agcttaggat ccatcgcccg actgtcgctg ttaatcaccc
     4801 atacgctggc atcaccgcga taaggcattt caaaggccac atcaactgta tgccccacct
     4861 gcgccaggct tacccccgcg tcgttggctc ccgtgaaatc atcggcaacg atgagaatgc
     4921 tcggcttagt gattcccttt tccatcatca ctccaggttg ttttatgata acaatgatta
     4981 tattcaatca tgattgatta aatgtgagca agctcaagtt attaatcact cgaataaatt
     5041 ataaaattag accatgttac gtaggctacg gcattttgaa tgattgaatt caatcaccag
     5101 aagagaggtt atttgtggta attatcaata gcacgatact gagcggcgca ggcgctatcc
     5161 cttccctgac gtcgctctta cccgacatca gaaaaatgct gctggtcact gaccgtaata
     5221 ttgcgcagct cgacggtgtg cagcagattc gcgccttact ggaaaagcac tgcccgcagg
     5281 ttaacgttat cgataatgtg cccgcagagc ccacgcatca tgatgtgcgc cagctaatgg
     5341 atgcccttgg cgatgcctct tttgatgtgg tggtcgggat cggcggtggc agcgtgttgg
     5401 atgtggcgaa gctgctatcg gtgctttgcc atccacaatc accggggctg gatgcgctgc
     5461 ttgcgggtga aaaaccgact cagcgggtgc aatcatggtt gattcctaca accgccggaa
     5521 ccggctcaga agccacgccg aatgcgattc tggcaatccc tgagcaaagc acgaaggtgg
     5581 gtattatttc ccaggtgctg ttaccagact atgtggcgct tttcccggaa ctgaccacca
     5641 gtatgcccgc gcatattgcg gcgtccacgg gcattgatgc tctttgccac ttactggagt
     5701 gttttaccgc gaccgtggca aatccggtca gcgataacgc ggcgctgact gggttaagta
     5761 aacttttccg gcacattcaa cccgccgtga acgatcctca ggatctgcgc gcaaaactgg
     5821 aaatgctgtg ggcgtcttac tatggcggcg tagcgataac ccatgcgggc acgcatctcg
     5881 ttcatgcgct ctcctacccg ttaggtggca aatatcatct gccgcatggc gtcgcgaatg
     5941 ccatcttgct ggcgccgtgc atggcgtttg ttcgcccctg ggcggtcgag aaatttgccc
     6001 aggtctggga ttgcattccc gatgcggaaa ccgccctgag cgcggaagaa aaatctcatg
     6061 ccctggtgac ctggttacag gcattagtca atcaactcaa gctacccaac aatctcgcgg
     6121 atctcggcgt accgccagag gatattgcct ctctgagcga ggcggcactg aacgtgaagc
     6181 gccttatgaa caatgtgccg tgccagcttg atctacagga cgtacaggcc atttaccaaa
     6241 cactgtttcc gcaacatcaa tttaaggaat aaatcatgcc gaaaaccatt gaaggtgtat
     6301 taaccgctat cgtcaccccg tttaccgcga ccggcagtct gaatatcccg gcgttaaaag
     6361 tgcaggtgaa tcgtcaactg gaagcgggta acgcgatttt ttgcggcggc accaacggcg
     6421 agttttttgt gttgaatgaa caggagaagc tcagcgttac gcaaacctgc gtggatgaag
     6481 tggctggtcg tgcgccagtc gtggcacaca ttggcgagat ctctacgcgc gaaacgattc
     6541 gtctgggtca gcagattgaa aaacttggcg tagatgccgt gtcggtcatt gccccttact
     6601 tcgtaccgct gaaacaggaa gagttgattg cgcattacag cgcaattgcc gacacactga
     6661 gcattccgat gtttctctat aacatcccgg cccgcactgg caatacgatc cagccagaaa
     6721 ccgcgcgtgc tctggcgtcg caccccaata tcattgggat taaagacagc gacggaagcg
     6781 acgaaagcct gaagggtttt ctcgatgcgg tgcgcgacat tgacggcttt aatgtgctca
     6841 acggcccgga ttcgctgatc cataaagggt ttgtcgaggg atgttcagcc tgtatctctg
     6901 ggctcgccaa cgtcgcgccg cgagaaatca atgccatctg gtctcgcttc catgccggtg
     6961 atattgaagg gtcttatgaa gcacaggaga gtgtaacggg cctgcgtacc gatctttata
     7021 aagtagcatt ctccccggct gcggtgaaaa aagcgttgca gcttatgggg catgatgttg
     7081 gtgatagccg ctacgcggtt aatttcacgc ctgaacagat cagcgagatt caacaaatta
     7141 ttcgtcatta taacattaat taaattaact ctattatcca gcccgctata aagtttagat
     7201 aaagagaata aaggattacc ccatgttttt gtggtttatg cagccagata tctggctcac
     7261 tttaattacc ctcacctttc tggaaattat tcttggggtg gataatatta tttttctttc
     7321 actggtggtg gcaaaacttc cgttatcaca acaaaatatg gcgcgtaaac tgggtttaag
     7381 ctgcgcgatg attatgcgga tattattgct gatatccatt gcgtggcttt cacatattac
     7441 ccagccgctt tttaccgtgc tgacgctgga gatttcgttc cgcacgttga ttctgctcgg
     7501 gggcgggctg ttcctgattt ataaagcgat acaggagata cgcgaagagc tgtcgcccgc
     7561 gcaggaagac gaacatggca gcggatcgcg gcaactgtcg ttgtgcggtg ccattgtaca
     7621 gataatgctg ctcgatatcg tgtttagcct ggattcggtg atcaccgccg tcggcctttc
     7681 tcagcatata tttatcatga ttgccgccgt aatgattgcc gtcggtgtca tgatgtttgc
     7741 ggcgaaaaca attggtgatt tcgttaacgc cacgccgtca ataaaaatac tggcgttaac
     7801 ctttttattg tttgtcggtg tattgctggt tgccgatagt ctcaacattc atattgccaa
     7861 agagtatctc tacttcgcga tatttttctc attaagtgtt gagacactca atattatccg
     7921 tgaaagaaaa aataaaaaag tttcgacatt gatcgaaacg catgaatcgc actcgcatta
     7981 ataaatctat taccaacttt aagatggagt ctaactatgt ctgtagccct tattcgtgac
     8041 ggcgtcatcc gtccatccga aaacgattcg catgtgctta ccgccatgct gccctcgtcc
     8101 tgcccgcaaa accacgcggc gaatattctg cctttgccgg atggcgcgtt aatgtgcgtc
     8161 tggtttgcgg gaacgcagga aggtattgcc gatatttctg tctggggatc gcgattgccc
     8221 gctggcggaa tgcagtggag cgatgcggcc aaactttccc acgatgacac gcgttcagag
     8281 caaaacccgg tgctgtttct cgcgccggat aacgtgctgt ggctcctatg gaccgcacaa
     8341 atatccggta atcaggacac cgccatcgtc cgctaccgta agtcagacga cctcggacaa
     8401 acctggggcg agattgccac cctgcttgat aaacccggta cgttcattcg acagccaatc
     8461 acggtgcttg ataacggcaa ctggctgctg cccgtctttt attgccgtac ccaacccggc
     8521 gaaaaatggg tcggcaatga tgatatcagc gcagtgaaaa tttccgctga cggtggtcac
     8581 tcctggcgcg atgtcgaggt gccgcaaagt ctcggctgtg ttcatatgaa tatcacaatg
     8641 ttgcacgacg gtacgttggt tgctctcttt cgcagccgtt gggcagacaa catctatatc
     8701 agccattcag tggacaacgg cgagagttgg tcggtgccgc aagcaaccga attgcccaac
     8761 aacaattcgt ctattcaggt aacgacgctt gccagcggcg aactggcgct ggtgtacaac
     8821 gcgatgagtg cggcaggcgc agtagaacgt cgcgcgtcgc tttatgacga aatcgacgat
     8881 ggcgacgaca gccgcaaaga accgacagcc gtcggacgct ccgctttctg gggtgcccca
     8941 cgcgcgccga tgacggtagc gatttctgcc gatggtggga aaagctggtc gtggcggcgc
     9001 aatctcgatg aaggcgacgg ctactgcatg accaacaatt ccctggaaaa gctaaatcgt
     9061 gagttctcct accccagcat caaacaaagc ccggacggta cgctacatat cgcttacacc
     9121 tggtggcgtc aggccatcaa gtacgtacgc atctcccccg agtgggttaa ggggcaggca
     9181 tcatgattat cggtaatctc aaccatttgt cgctggcggg gcttcctgcc tgggtgagaa
     9241 atattctgct gcgcccggag tgttcgttat cggcgttatc caccagagag gacggtcgct
     9301 ggcaacctga aggatgtcgg tggttctgct cgctgggaac ttcagacacg caacccgccg
     9361 aacttcgcca tacggagtat caccatctat gggccgatat acaggtggtg attactggtt
     9421 gtgagggcat taatgcggga acgcggccca tcgctcgcga aaacgatgaa gaacgtaagc
     9481 ccgatctgtt tatcgcccca tcccctgaaa acagtgtcgc catcacccta cacgccggtg
     9541 atttcgccgt ctttatgcct gaggaacctc accaggcact gtgcgcaatt ggcacggcta
     9601 cttcaatacg caaagcggtc tttaaagtac cgcgcgacat gctggaagcc taaaaggaga
     9661 gaaaaaatga caatggaaat gatcctggca ttaggaatac tggtcctgat gatcgtgctg
     9721 atcatgtcgg acaaaatgcc ttttggcgca ccgccactgc ttgcgtgttt attgctggtt
     9781 gtatcgggac tttcgaccgt tcaacaggct tttgccggat tcgtcaatcc aagcgtggtg
     9841 atgattgcag ggtttatggt ggtcatggcg gcgctacaaa aaacccggct tatcagcaac
     9901 gttaaatctg cgatgataag cctggtcaat aaaggcagtt accgcagcta tgggctgttg
     9961 ttagtcattg ttatgctggg tgccagtctt gccggcaccg gagcgaccgg ttattacgtg
    10021 ctaattctgt cgctggtatc caccattccg tacagtaaaa agctaccaac atcgaaatta
    10081 atgatgcccc tgggatttgc caccaaccac ccgcttcttc ctatcaatct cgcgcttctc
    10141 tttggcgtta cggcgaccgt actcgaaacc gcggggtttc atcaggcgat ttcgatgggg
    10201 cgctttgcgc tggtgaatct catcatgtca gcggctttcc tcgcatggag tcttattgcc
    10261 taccgttttc ttccggatca tcccattgcg gatgcttccg aggatgcgct ggcggcacgc
    10321 gaggaaacgt ttaacgccct tcctgcctgg aaagaatatt gcactattgc cgcattcgct
    10381 gtcagcgtta tcggcatgat gctaatgaac attctgggaa atatcgcctt cgttattccg
    10441 ggtcttgcgg gtgcgtttgt tctgatgatc gatgtactgg acttcaaaga agttcgcgat
    10501 catatggggg ctccggtcat tttaatgatg gcgggggtga tcggtgtagc tgatgcactg
    10561 gcgggcaccg gctttacggc gatggttggc gatgccgttg caggtgtgct cggttctggc
    10621 gtctcaccgt ttgtatttat tgtcgccttt gctttgctta ccagtacctg cgccaccttc
    10681 acgggttcga atatggggtc agtatatatt tttgccccca tcgccattgc cgcctgtact
    10741 agccttgggc ttaacccgac cgctgccgcg attgcggtgg tgatttctgg ctggaatggc
    10801 ggctatatgc ctattgacgg aatgccagcc atgatcctcg gaatggggaa atacaaactt
    10861 cccgaattct gggtgttctc tgtaccaatg tatctcatcc gcattctggc gctttgtgca
    10921 ggcgctgttt ttatcttccc aatgtaatct cagacaaaaa aagcggcaga gaagatccct
    10981 gccgcttttt attgttggat gcgacgctgc cgcgtcgcat cagacataaa gcagattact
    11041 ttttgatttc atacagcggt gtttgacccg ccacaacatg gccctgagct ttaatgataa
    11101 ggccgctgaa atcatcgata ttgctgcaaa ccaccgggct aatcatcgaa cgggcgttag
    11161 cgttcaggta atccagatcc atttccagaa tcggttgccc tgcgcttacc tgcgcgccct
    11221 cttccaccag acgtttaaag cctttacctt ccagcgctac ggtgtcgata cccatatgga
    11281 cgacgatctc cgcgcctttt tcggtttcca gacagaacgc gtggttggtg ttgaagattt
    11341 tcacgattgt tcctgcggct ggtgatacga cgattttatc tgtcggtttc accgccacac
    11401 cgtcacccac cgctttgctg gcgaatgctt cgtcaggaat ctgatccagt gccacaacat
    11461 ccccggtaat cggcgatacc agctccgcga tagataccgc gtttggtaca gcctgcggtt
    11521 ttgctacagg agcggcagtt gccggagttg cttcagctga cgcagcggct accggaccac
    11581 gggcaacgac tttcttcatc gcatcgccga tggattctgc tttcgcgcca acaatcacct
    11641 gaatagtctg tttgttcagt tttactaccc cagaagcacc cagacgttta cacatcgcat
    11701 cgttgacgcg agctgagtca accacggtaa ggcgcagacg agtaatacag gcgtcaatgg
    11761 ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt ggttgccagt tgattgagac
    11821 cttcttcagt attgctgtta gcctcttcag taacgatctc gtcttcttta tcttcacgac
    11881 ccggcgtttt caggttgaac atgcggataa ccaaactgaa caccacgaag tagatagcga
    11941 agaagacaac ccccatcacc agcagcatcc agacgttctg gctggcggcc ggcaggttat
    12001 acatcaacgc gtagtcgata gcccccgcag agaaagagaa gccggcatgg atacccagca
    12061 acgttgccac aaacaggctg ataccggtca gcagtgcgtg caggaggtac agcagcggtg
    12121 caaggaacat gaacaggaat tccagcggct cagtcacacc ggtcaggaac gcagtaacag
    12181 caacagaaag cagcatcccg ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca
    12241 tcgccagcgc cgcacccggc aggccgaaca tcataatcgg gaagaagccg gacatgaaca
    12301 tccccgcggt gccgtcaccg gcatagaagc ggttgatatc accgtggaaa accgttcccg
    12361 ccgcgttggt gaattcacca atctggaacc aggcgatggt gttcagcacc tgatgcagac
    12421 cggttgggat cagcagacgg ttgatgaaac caaagatacc ggaacccagc gcgcccgcag
    12481 aaacgatcca ctcgccgcct gcatggatag cgtgctgtac cggtggccag acgtaaccaa
    12541 aaatggccgc cagcaccaga cagaagaagc cggtggctat cggcacaaag cgtttgccgc
    12601 cgaagaagct caggaagtcc ggcagtttaa tatcggacca acggttatag gctgcgccac
    12661 caaccagacc ggtaatgata cccgccagta cacccatgtt aatttctggg ttgatggtca
    12721 ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc cagtgccgcc gcacctgcgc
    12781 tgtctttcga ccagctggat gccacgccga tggcgaatat taatgcgagg ttatcaaaaa
    12841 tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa atctggctga ccgaatcgca
    12901 gcaacagcgc cgccaccggc agcaccgcga tagggagctg taacgcccta ccgagtcgct
    12961 ggaaaaaacc taaaatattc atcttattcc ccctacgaga accctatttg gctcgtttaa
    13021 agccgtattt ttattttgct gcaaattgta ctgccgatgt tctgtaatca gattgttaga
    13081 tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt
    13141 gtgagttttg tcaccaaata tcgttatcat cactcccttt tactggctaa accagaaaac
    13201 ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac
    13261 gcttaaagat gcctaatccg ccaacggctt acattttact tattgaggtg aataatgaga
    13321 ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat
    13381 cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg tactgggcct gccgactggc
    13441 ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc
    13501 tttaagcacg ttgtcacctt caacatggac gaatatgtcg gtctgccgaa agagcatccg
    13561 gaaagctact acagctttat gcaccgtaat ttcttcgatc acgttgatat tccagcagaa
    13621 aatatcaacc ttctcaacgg caacgccccg gatatcgacg ccgaatgccg ccagtatgaa
    13681 gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg taacgacggt
    13741 catattgcat ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat caaaaccctg
    13801 actcatgaca ctcgcgtcgc gaactctcgt ttctttgata acgatgttaa tcaggtgcca
    13861 aaatatgccc tgactgtcgg tgttggtaca ttgctggatg ccgaagaagt gatgattctg
    13921 gtgctgggta gtcagaaagc actggcactg caggccgccg ttgaaggttg cgtgaaccac
    13981 atgtggacca tcagttgtct gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct
    14041 tccaccatgg agctgaaagt taagacttta agatatttca atgaattaga agcagaaaat
    14101 atcaagggtc tgtaattgtt atccctgccc tggctccttg ctcagggcaa tattttttaa
    14161 aatcgggggt cagaatgtat gcattaaccc agggccggat ctttaccggc cacgaatttc
    14221 ttgatgacca cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg
    14281 aactgccgcc agagatcgaa caacgttcac tgaacggggc cattctctcc cccggtttta
    14341 tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa cgacaccgct gaagcggtca
    14401 gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa atcaggctgt actaactatc
    14461 tgccgacgct tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg
    14521 agtacctggc aaaacatccg aaccaggcgt taggtctgca tctggaaggt ccgtggctga
    14581 atctggtaaa aaaaggcacc cataatccga attttgtgcg taagcctgat gccgcgctgg
    14641 tcgatttcct gtgtgagaac gccgacgtga ttaccaaagt gactctggca ccggaaatgg
    14701 ttccagcgga agtcatcagc aaactggcaa atgccgggat tgtggtttct gctggtcact
    14761 ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc tttgccaccc
    14821 atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc
    14881 tcgacgaagc tgacatttat tgcggtatta tcgctgatgg cctgcatgtt gattacgcca
    14941 acattcgtaa cgctaaacgt ctgaaaggcg acaaattgtg tctggttacc gatgccaccg
    15001 cgccagcagg tgccaacatt gaacagttca tttttgcggg taaaacaata tactaccgta
    15061 acggactttg tgtggatgag aacggtacgt taagcggttc atccttaacc atgattgaag
    15121 gcgtgcgtaa tctggtcgaa cattgtggta tcgcactgga tgaagtgctg cgtatggcga
    15181 cgctctatcc ggcacgtgcg attggcgttg agaaacgtct cggcacgctc gccgcaggta
    15241 aagtagccaa cctgactgca ttcacacctg attttaaaat caccaggacc atcgttaacg
    15301 gtaacgaggt cgtaactcaa taagagaaag tatgacacca ggcggacaag ctcagatagg
    15361 taatgttgat ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga ttgaccagta
    15421 cgggccaatc tcgcggattc agattgccga gcaaagtcag cttgcccccg ccagcgtaac
    15481 caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc
    15541 caccgggggc cgccgcgcta tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg
    15601 cgtacggctt ggtcgtcacg acgccaccat caccttgttt gatctcagca gcaaagtgct
    15661 ggcagaagaa cattacccgc tgccggaacg tacccagcag acgctggaac atgccctgct
    15721 gaatgccatt gctcagttta ttgatagcta ccagcgcaag ctacgcgagc tgatcgcgat
    15781 ttcggtgatc ctgccagggc ttgtagaccc ggacagcggc aaaattcatt acatgccgca
    15841 tattcaggta gaaaactggg ggctggttga agcactggaa gagcgtttta aagtgacctg
    15901 ttttgtcggc cacgatatcc gcagtctggc gctggcagag cactacttcg gtgcaagtca
    15961 ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga accggggccg ggattatctc
    16021 taacgggcga atttttatcg ggcgcaacgg caacgtcggt gaaattggcc atattcaggt
    16081 cgaaccgctg ggtgaacgct gccactgcgg caactttggc tgcctggaaa ctatcgccgc
    16141 caatgctgcc attgaacaac gggtgttgaa tctgttaaag cagggctacc agagccgtgt
    16201 gccactggac gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag gcgatagcct
    16261 ggcctcggaa gtgattgaat atgtcggtcg tcatctgggt aaaaccatcg ccattgctat
    16321 caacttattt aatccgcaaa aaattgttat tgctggagaa atcaccgaag ccgataaagt
    16381 gctgctccct gctattgaaa gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa
    16441 tctaccggtg gtacgttctg agttggatca ccgctcggca atcggcgctt ttgcgctggt
    16501 aaaacgcgcc atgctcaacg gtattttgct ccagcatttg ctggaaaatt aatgtgcttt
    16561 tatagtggcg cttattgttg tcaatattct gggtagtcca tgaccattaa aaatgtaatt
    16621 tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg taccgggtgc agcggaattt
    16681 ttgcacggga ttatggataa aggcctgccg ctggtgttgc tgaccaacta tccttcgcag
    16741 accgggcaag atctggcgaa tcgctttgcc actgccggtg tcgatgtacc ggatagcgtg
    16801 ttttatacct ccgcaatggc gaccgccgat tttctgcgtc gccaggaagg caagaaagcg
    16861 tatgtggtgg gcgaaggcgc actgattcat gaactgtaca aagccggttt cactattacc
    16921 gatgtgaacc ctgattttgt gattgttggc gaaacgcgtt cctacaactg ggacatgatg
    16981 cataaagcag cctatttcgt cgctaacggt gcacgcttta tcgccaccaa cccggacacc
    17041 cacgggcgcg gtttttatcc cgcctgtggc gcgttgtgtg cagggatcga gaaaatctcc
    17101 gggcgcaaac cgttctatgt tggaaagccg agtccgtgga tcatccgcgc ggcattaaac
    17161 aaaatgcagg cgcattcgga agaaacggtg attgtcggcg ataacttgcg taccgatatt
    17221 ctggcgggct tccaggccgg tctggagacg attctggtgc tttctggtgt ttcgtcgctc
    17281 gacgatatcg acagtatgcc gttccgccca agttggattt acccatcggt cgctgaaatc
    17341 gacgttatct gaatagcaac cacgcctcag ggcgtggttt cttattttta cccatcatta
    17401 aaatcacccc attcaataaa acccgtcaaa aactatcgat ccatcaataa atatacccaa
    17461 aagccctccc ctttttcatc aatcagcaat cgccattacg ttttttattt ttccactgcc
    17521 aaatcgcttg cgcaccgtgc tgctttgcgg cattttttta acaagtaaac acaacacgca
    17581 gtaaacacca ggttaatgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
    17641 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
    17701 ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgccca cgaacgtctg
    17761 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aactcatgtg
    17821 ctggcagtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt
    17881 taccagttcc agaccgggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
    17941 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
    18001 gatgcatatc tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac
    18061 gaacacggtc agctgtatgt ggcctcagaa atgaaagccc tcgtgccagt ttgccgcacg
    18121 attaaagagt ttccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat
    18181 tatcatcgcg actggttcga ctacgatgcg gtgaaagata acgtgaccga caaaaacgag
    18241 ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt
    18301 gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgcc
    18361 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt gcactccttt
    18421 gctgtaggtc tgccgggttc accggatctg aaagcagccc aggaagtggc aaaccatctg
    18481 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
    18541 gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat
    18601 ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct
    18661 gacgaagtgt ttggcggtta tctctacttc cacaaagcgc cgaacgctaa agagttgcat
    18721 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg tgccaacaaa
    18781 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
    18841 gtggcgatgc gcattaaccc acaggataaa atgtgcggta acggcaaaat ggaaaaacac
    18901 atcctgcgtg aatgttttga agcgtatctg cctgcaagcg tggcctggcg gcaaaaagag
    18961 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcagcgcag
    19021 caggtttctg atcaacagct ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc
    19081 tcaaaagaag cgtatctgta ccgggagatc tttgaagaat tgttcccgct tccgagcgcc
    19141 gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt ccgctaaagc gattgagtgg
    19201 gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg cggttggcgt tcaccagtcg
    19261 gcatataaat aagacgtgta atattgcccc ggagaaatcc ggggcaattt cagttactgg
    19321 ggcgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac atcgctattt
    19381 gacaactcat cactgtaggc caccaaccag gtcgcctgct catccggaat atgcagtctg
    19441 ccatttccct ctttcaggcg taataactca aaagatttgc ccttaattac ggcggtatag
    19501 gatttctcac cttcctgtaa tgctttcact ttacagcgaa atatctgacc atcgatgtca
    19561 ttatcaacat gcgatacctg aatgagggct tcattagaag ctttcgggcc tatccgcatc
    19621 atccacacca cagcgccttc ctgcccacga tagccaaaaa catatttacc gatttccggt
    19681 ttaaccgtat cggcgaacgc agattgcata agtaataacg cacccatcaa cgccgtcata
    19741 aaacgcagca tagtattccc tcaataaatc gcctgaagtg gtagcagaga tgcaggcgta
    19801 gcgatccctg ccctgtcctg ttgcaacggg ccagtatttc atagattaat ttcccaccac
    19861 tattgttgaa ttccgaattt catcctgtat gacaaaagaa tggctgtagg gtggggaaaa
    19921 aacacaaagt aaacaataat tgacgaatat agcgccatgc tgttcgcaac ctaaccaaac
    19981 agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat
    20041 aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa agatggctac gtagctcagt
    20101 tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc
    20161 atcttttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    20221 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    20281 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    20341 aggtacccca gccatattct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
    20401 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    20461 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    20521 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc
    20581 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    20641 tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga cgcttacctt
    20701 gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    20761 tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg gcgagctttt
    20821 tgcttttctg cgtatattca atgccgaata cgatgttgac tcgtcttatc cttacaaagt
    20881 cggatgcgac gctgccgcgt cttatccgac ctacggttgg catgcatcca gcaatgttgt
    20941 gaggctacaa ccctaacgca tatttcagcg ccttacgttt caacacgcca gcacgctccg
    21001 ccgccatcaa accaagatta cgcacaaaac gcagcggtgg cagattattg ctgaatccgg
    21061 cataaaacag atccatacca ctttgcatca tgaagttatc cgccatacgc cgcatctggt
    21121 aacgcttgag cacaggataa ctggcccacg cttcgccgta gctgcgggcg ttcaccagaa
    21181 cgtcaatcag ggcatcgaca tcacgataac caagattcac tccctgcccc gccagcggat
    21241 ggatggtatg cgcggcatcg cccaccagag caagccctgg ttgcacatac tgcaacgcat
    21301 ggcgacgtat aagcggaaac gcaccagcgg caagcggtgt cacgtaaccc agacgcgacg
    21361 ggaaatgctt cgcgatttcc gcctgtagct gcgccatatt catattctgc aactggcgaa
    21421 tgcgcgccgg agagtcatac cacaccagcg atgcccagtt atcaaacaac ggcagaaacg
    21481 cacgcggtcc gtccggggta aattgctgcc aggtgctgtc gccgggatcg ttctcacact
    21541 gtacgctaat caacatacac gactgcgcat actgccaggc atgaacgcca attcccgcca
    21601 tctgtcgcac ctgcgaattt gcgccgtcgg caccaatcac cagcttcgcg aggatcgttt
    21661 caccgccctt cagttccagc tcctgaagat cattatggcg atgcagtgca gtcagcgagg
    21721 ttggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaaa gcctgttgca
    21781 ggacagtgtt ttccaccatg tagccaagca acggtagctt aagttcagcg gcgtcaaaca
    21841 cgacatgcgc cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg caacgcatag
    21901 cctgtactgc atcccagacc cctaaccctt taagcaatga taccgaagcc gcgctgatcg
    21961 ccgagatccg cacatccggt tggctgtcag cgacaaacgg cgcaggttct gcgtgctcga
    22021 tcaccgttac cgtaaacccg tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc
    22081 ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat cctgttagca
    22141 atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca tactggtcac
    22201 aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt cgcaagagca
    22261 agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac gagtacgatt
    22321 catcgaagat ggccgatctg ctggatgcca cccacggcta tcaactgacc gacgtggcgg
    22381 aagaagcgga tgtgctgctg ctgaacacct gctcaatccg cgagaaggct caggaaaaag
    22441 tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac ctgattatcg
    22501 gcgtcggcgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc gcccactatg
    22561 tcgatattat ttttgggccg caaacgctgc atcgtctacc ggagatgatc aactccgtgc
    22621 gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag tttgaccgtc
    22681 tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa ggctgcaata
    22741 aatattgcac ctactgcgtg gtgccttaca cccgtggtga agaggtaagc cgtccgtccg
    22801 acgatattct gtttgagatc gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc
    22861 tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga cggcaccacc ggaacgtttg
    22921 ccgatctgct gcgtctggtt gcggcgattg acgggatcga tcgtattcgc tttaccacca
    22981 gccatccgat cgaattcacc gacgatatca tcgaagtgta tcgcgacacg ccggagctgg
    23041 tgagcttcct gcacttgccg gtacagagcg gttccgatcg cattctgaac ctgatgggcc
    23101 gtactcacac ggcgctggag tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccgg
    23161 atattcagat cagttctgac ttcatcgttg gcttccctgg cgaaaccacc gacgacttcg
    23221 agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc tttatcttct
    23281 ccgctcgtcc aggaacgcct gccgccgata tggttgatga tgttccggaa gaagagaaga
    23341 agcagcgtct gtatattctg caagagcgca ttaatcagca agcgatggca tggagccgcc
    23401 gtatgctcgg caccacccag cgtattctgg tagaaggtac atcgcgtaag agcatcatgg
    23461 agctttccgg tcgtactgaa aataaccgcg tggttaactt cgaaggcacg ccggatatga
    23521 tcggtaaatt cgtcgatgta gaaattaccg acgtctatcc aaactccctg cgcggaaaag
    23581 tggtgcgtac tgaagatgaa atgggtctgc gcgtcgcaga aacaccggaa tcggtgattg
    23641 cccgtacccg caaagaaaac gaccttggcg tgggttatta tcagccgtaa ttctcaggcc
    23701 cgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg caatgcttgc
    23761 gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca gcttccggcg
    23821 gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc tggagccagc
    23881 agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca aacaactcga
    23941 acgccgtctc ggcatcgaaa tcaatcgccg tgataaccac tttaaactga ccggccgtcc
    24001 gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata ctgccccgat
    24061 gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta aagaagcgcg
    24121 ggtactggag caaagcgcgg agagcgtgcc ggagtacggc aaagcagtca atatcaaaac
    24181 caaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct
    24241 cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct acctggcagt
    24301 ggctgcggca gttgatgccc tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc
    24361 ggcggtcgaa gccggtgaga aactgggctt cctgcctggc gatttaagcc agaaagtaga
    24421 cccgtatctg cgcccgctgt acgacgcgct gtttgaaatg ctgggctttg agaaagtcga
    24481 gaaactgatt gagcgcaacg ttattgaagt cgcaccgctg gcctatatgc gtggtcgtac
    24541 gctgaacgac gcgtttatca ttcttgatga gagccagaac accaccatcg aacagatgaa
    24601 gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggcg acgtcacaca
    24661 gatcgacctg ccgcgtaata ccaagtctgg tttgcgtcat gccatcgaag tgctggctga
    24721 tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgtc acccggtggt
    24781 ggcgcgtatc gttaacgctt atgaagcctg ggaagaagcc gaacaaaaac gtaaagcggc
    24841 gctggcggca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat
    24901 ttacaactgg catgtgaaga taattccggg ttaccggaag agagccagtt tcagacatgg
    24961 ctgaatgcgg tgatcccgca gtttcaggaa gaatcggaag tgactattcg cgtggtcgat
    25021 accgccgaaa gccacagtct gaatctgacc tatcgcggta aggataagcc gaccaacgtg
    25081 ctctccttcc cgtttgaagt gccgcctggc atggaaatgt cgctactggg cgatctggtt
    25141 atctgccgtc aggtggttga gaaggaagct caggagcaag gcaaaccact ggaggcgcac
    25201 tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca catcgaagat
    25261 gacgaagcag aagaaatgga agccatcgaa acagagatta tgcttgctct gggctatgag
    25321 gatccgtaca ttgccgagaa agaataattt gccaggcatt tgactggcag acgtccccgc
    25381 cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac gacgccatga
    25441 gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt ttctccctgt
    25501 tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc
    25561 gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc gaaggggtga
    25621 tggacatcgc agaccaacgc gttcgcgaca tcatgatccc ccgctcccag atgattaccc
    25681 tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc gcccactcac
    25741 gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg gcgaaagact
    25801 tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg
    25861 cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt cgctctcagc
    25921 gttaccacat ggcgatcgtc attgacgaat tcggtggggt ttccggtctc gtgaccattg
    25981 aagacatcct ggaactgatt gttggtgaga ttgaagacga gtatgacgaa gaagatgata
    26041 tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct tctattgaag
    26101 acttcaacga agcgttcggc acccacttta gcgatgaaga ggttgacact atcggtggtc
    26161 tggtgatgca ggcatttggg catcttccgg cgcgtggcga aaccatcgac atcgacggtt
    26221 accagttcaa agtggcgatg gccgacagtc ggcgtattat tcaggttcat gtcaaaatcc
    26281 cggatgactc accccagccg aagctggatg aataaaaccg aaactggata gataactaca
    26341 tggcttttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg ttattattcg
    26401 gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg gcctgcggcg attatttcgc
    26461 tgatgggact tcaggcgctg acctttaacc gccgtccact ccagtctacc gctattggct
    26521 tttgctgggg atttggcctc tttggcagcg gtattaactg ggtctatgtc agcatcgcga
    26581 cctttggcgg aatgcctggc ccggttaaca tcttcctggt ggtactgctg gcggcgtatt
    26641 tgtcgctgta taccggactg tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct
    26701 ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt
    26761 gggtacttac aggcttcccg tggttacagt tcggctatag ccagattgat ggcccgttaa
    26821 aagggctggc accgataatg ggcgtggaag ccattaactt cctgctgatg atggttagtg
    26881 gcctgctggc actggcgttg gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc
    26941 tgtttgccct tcccttcccg ctgcgttacg tccagtggtt taccccgcaa ccggagaaaa
    27001 ccattcaggt ttcgatggtt cagggcaata ttccgcaatc gctgaaatgg gacgaaggcc
    27061 agcttcttaa tacgctgaag atttactaca acgcaacggc accgctgatg ggcaaatcat
    27121 cgctgattat ctggccggag tcggcgataa ccgatctgga aattaatcag caaccgttcc
    27181 tcaaagctct ggacggtgag ttgcgcgata aaggtagctc gctgatcacc gggattgtcg
    27241 acgcgcgtct caataaacag aaccgctacg atacctacaa caccatcatc acactgggta
    27301 aaggcgcacc gtacagctat gaatcagccg atcgctataa caaaaaccat ctggtgccgt
    27361 ttggcgagtt tgtcccactg gagtcgattc tgcgtccatt agcaccgttc tttgatttgc
    27421 cgatgtcgtc attcagccgc ggaccatata ttcagccacc gctgtcgttg aatggcattc
    27481 aacttactgc ggccatttgc tacgaaatca ttctcggcga gcaagtgcgc gataacttcc
    27541 gcccggatac cgactatctg ctgactatct ccaacgatgc gtggtttggt aagtctattg
    27601 gtccatggca gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt
    27661 tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag attcaggcga
    27721 tgatcccgca gttcacccgc gaggtgttaa ccactaacgt aacgccgacc accggactca
    27781 ccccgtactc acgtaccggc aactggccac tgtgggtgct gacggcactg tttggctttg
    27841 ccgctgtact gatgagtctg cgtgctcgca aacgttaatg tgcctgatgc gcgacgctta
    27901 tcaggcctac gcgatttttg caatatgttg aattttcgcg attttgtagg ataaagcgta
    27961 ctttttcaac acgctctggg gcatgattat catgcccttt tttcttacgg ttcagggata
    28021 caagccacat cgccatattg gggatgccga ataattgcct gcggatcaag tgaactttct
    28081 ctgtccagtt gtattagccc gttcttcgcc ataacaaata tctttctacc atccgctgaa
    28141 aaacgaagat gattcacgta gctggcagga taaacgccaa taatctgccc ggcattaata
    28201 ttgagcagga tgacataacc ttgcaggcca tatgacacca acgctaaata ttgaccgtcc
    28261 gggctaatac cctcaacgga ataaacgctc ctgctacacc attgcgtcat aatgctggct
    28321 tgttgatcct cagctcttat acgtgaaatc ccctggctat cggcaaaaat aatctcacca
    28381 ttttttggtc ggaatcccat cgcagaaacg acaccacgcg catcacctgc gggaaagtct
    28441 tctttctgat gcgtgatggt attgatccgc acgatgttac ctctgcctgc tcccggaacc
    28501 agtaatgttt tttcatccgg ggaaagtgcc ataacccagg cgcctggttt accgctcatc
    28561 cccgtcccgt cgtatttctc cagcgggata tccgccacat gctgataagc caccagatca
    28621 atcgctgaca cactattact ggtgcgattc atcacccagg cttttgtgcc atcacgcgag
    28681 aggataaggc tgtcaggatg tgcgccggtg gtaatcgact gaataatctc ccccgttgcc
    28741 acgttaatga ccgataatgc attcgcttca aatgaagtcg catacagcaa cgtttcatcc
    28801 gggcttagcg ccagcgacct gacgccctga tctttacagc caccacaatg tttgactgac
    28861 ttttctttcg ctatgggggc tgtaaaggta tgcaggattt tcttttgtcc gaggtcgaaa
    28921 acaacaatgc tgttcgtgtt ttcaattgcg gcatacaacg tattagcatc ccgactgatc
    28981 gccatatcat ggatatcggc aatctcagcg agtaaatcac cactggcctg ctcacgactt
    29041 accggagcgg ataatgtttc cctgagggcg ttgcgctcct ccactaactg ctgctttttt
    29101 tgtcgtcgct cttgttcaat ctcctgcgga taaaacgctt cacgcaggct ttgcctgacc
    29161 tgtaaaactg attcatcaat ctgctgctgc acaagccaga tagtcccggt cagaagaaga
    29221 gtgaccgcaa caataatccc gccttccgta acgagccgat tttgcccaac taaacgcgca
    29281 ctcagccaac ccaacgcgcc ccccgcaatc aggccgccaa tgtggcaagc gttatctatt
    29341 cctgtttgcc gggtaccata caacagtgtc agcgccacca tacccaacag aggaaatact
    29401 cgccgctggt ttttatggtg tgtgcccgca cctgaagcaa gctgtgttgc tatcgcagcg
    29461 cccgctattc ccataattgc cccggatgcc ccaacgctga tgactatctg ctcactttcg
    29521 cgtaacgcat aacaggcgct gatcagccca ccaaagatgc cggagaataa ccagataatc
    29581 aggagtcgcc atttccccag tatccgttcc gccactgtcc ccaccaccag gagtgcaaac
    29641 atattcatca gcaaatggga aaaactactg tgcagaaaaa cactgctcaa taagcgccag
    29701 gattcatccg acaacgtgag agaagcaacg ttagcgccga gatggatgag tgcggattga
    29761 tcgacttctt cgtaactcat ccattgtgcg aggtaacaaa atatcccaac acagagcgca
    29821 aaaaggatca gggttattgc gggtaattga acatttaacg gcttaaccga agatgcagac
    29881 ataaggtgat tcttccttga aaagactagt aaaaagcatt ttcccgtgtg tagttatcat
    29941 cccggcaaac gtcccacacc gaaaagttaa cactgtccga aatatgactt tttcaaactg
    30001 aaattacaat cgtaattaat tcacccctaa gtattttttg gcatgtaagt tgctttattt
    30061 actgtgtaag caatgaaatc agccgcaacg caaccgggtt gcaccaccac aaatcagcgg
    30121 tagcaccaaa aaggtgcggt ttaagaattt tgcttcggaa tggtgcggcg cacctcgcaa
    30181 aaacaaacaa taaaacagca atatctttac atttatccaa actaaatgtt acctaacaag
    30241 cacaacactg cacaataaag tcgcagatga taacaacaca aacactcaca acgggtatcc
    30301 atgcgttttt aacgcagaag ataaaggagt tggatatgca attacgtaaa cctgccacag
    30361 caatcctggc cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag
    30421 ctggcaatac gctggacaaa atcgccaaaa acggcgtgat tgtcgtcggt caccgtgaat
    30481 cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggctac tcgcaggatt
    30541 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccagac ttacaggtga
    30601 aattgattcc gatcacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt
    30661 ttgaatgcgg ttctaccacc aacaatgccg aacgccaaaa acaggcggct ttctctaaca
    30721 ctattttcgt ggtcggtacg cgcctgctga ccaaaaaggg cggtgatatc aaagattttg
    30781 ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac cacctctgaa gttttgctca
    30841 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
    30901 actctttccg taccctggaa agcggtcgtg ccgttgcctt tatgatggat gatgctctgc
    30961 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc
    31021 agtctcagga agcctatggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga
    31081 tggatgatac catcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataagt
    31141 ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa
    31201 tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg
    31261 cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc ctggtggtcg
    31321 ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta
    31381 acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt
    31441 tttttacaac aagccccgtt cggcaacacc acctatcttg gttggatctg gagtggcttt
    31501 caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt
    31561 ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc
    31621 gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtacct agtgatcccg
    31681 gagctgctgc cagagaaaat cggcatgtgg tttaaggcag agctggatcc caatattcag
    31741 tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag
    31801 gtacgcgcag ctattcagtc gctgccgcgt gggcaaaaaa atgccgcgct ggcgatgggt
    31861 ctgacgctac cgcaggctta ccgctacgta ctgctgccta atgcttatcg cgttatcgtc
    31921 ccgccgatga cctcagagat gatgaacctg gtgaaaaact ccgccatcgc ctcgactatc
    31981 ggtctggtgg atatggcggc gcaggcgggt aaattgctcg attactcggc ccacgcctgg
    32041 gagtcgttta ccgccatcac gctggcgtat gtattgatta acgcttttat catgctggtg
    32101 atgacgctgg ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa ataatgtacg
    32161 agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca
    32221 cgctgaaaat caccgtcacg gcagtggtga ttggtatttt gtggggcacc atgctggcgg
    32281 tgatgcgctt gtccagcttt gcgcccgtag cgtggtttgc caaagcctac gttaacgtat
    32341 tccgctcgat tcctttagtt atggttttgc tgtggtttta cctgatcgta ccgggttttc
    32401 tgcaaaacgt gctgggatta tcacccaaaa atgatatccg tttaatttcg gcgatggtgg
    32461 cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgctggt attcaaagta
    32521 tttctcgtgg tcagtcgagc gccgcgctgg cgttgggaat gactcactgg cagtcgatga
    32581 aactgattat tctgccgcag gcgttccgcg cgatggtgcc gctgttgctc actcagggca
    32641 tcgtactgtt tcaggatacc tcactggtgt atgtgttatc tctggccgat ttcttccgta
    32701 ccgcctcaac cattggtgag cgtgatggta cgcaggttga gatgatcctg tttgccggat
    32761 ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga
    32821 cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac
    32881 cgactgctca accgaagtga aaaaaggcga agttgtggtg gtttgcggcc cgtctggttc
    32941 cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccggtgcagc aaggtgaaat
    33001 caccgtcgat ggcatcgtgg ttaacgacaa gaaaaccgat ctggcgaaac tgcgttcccg
    33061 cgtcgggatg gtgtttcagc atttcgagct gttcccccat ctgtcgatta tcgaaaacct
    33121 gaccctggcg caggtgaaag tgcttaaacg caataaagcg ccggctcgtg aaaaagcatt
    33181 gaaattgctg gagcgcgtcg ggctttccgc acatgccaat aagtttccgg cgcagctttc
    33241 cggcggccag cagcagcgtg tggcgatcgc tcgcgcattg tgcatggatc ctattgccat
    33301 gctgttcgac gaaccaacgt cggcgctgga tccggagatg atcaacgaag tgctggacgt
    33361 gatggtcgaa ctggcgaacg aaggaatgac gatgatggtg gtgacccacg aaatgggctt
    33421 tgcccgtaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaatta tcgaagactc
    33481 gccgaaagac gctttcttcg ataatccgaa atcggaccgc gcaaaagact tcctcgcaaa
    33541 aatcctgcat taatcactct ggcgcgcata ctcaattgcg cgccatctca cgctttctcg
    33601 tttcgtccct cgtatctgtt acatcccggc gttacccttg tcgcaaagaa acacacaaca
    33661 aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg acgacgctat
    33721 cgcaatagtt ctcgccctcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc
    33781 cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa
    33841 tcgcaccgat attccggtag caagcggtgc ggtaaaaccg ttaatgcgta atttgattat
    33901 tgcggacaac gtgcacggcg aaagcggcct ggacggcccg gcattgccgg aaccggcatt
    33961 tgcaccgcaa aactgcacgg cagtggagct gatggcgaaa acgctgcgtg aaagtgcgga
    34021 acctgtcacc attgtttcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca
    34081 cccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg
    34141 taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt
    34201 cttccagtca ggaattccgg tggtgatggc cggtctggat gttactcaca aagcacaaat
    34261 ccacgttgaa gacactgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc
    34321 cgaactgctg gatttcttcc tcgaatatca caaagacgaa aaatggggct ttgtcggcgc
    34381 accactgcat gacccatgca ccatcgcctg gctgttgaaa ccggagttgt ttaccactgt
    34441 tgagcgctgg gttggcgtgg aaacacaagg gaaatatacc cagggtatga cagttgttga
    34501 ttattattat ctgactggca ataaaccgaa tgccaccgta atggtcgatg ttgatcgtca
    34561 gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaacata cgtcattgat
    34621 taagatcacc acgcattacc tgggtggtct taatctggtt atctcttttt ctttcaagat
    34681 ataaatgcaa tggataatgc agaacttgct attggtatcg atctcggtac taccaatagt
    34741 ttaattgccg tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat
    34801 ttaacaccat ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct
    34861 gtatcacggc ggacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc
    34921 agtaatacca actggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg
    34981 gtattacgct cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg
    35041 gtgatctccg ttcctgctta tttcagcgat gaacaacgca agcatacccg tttagcagca
    35101 gagttagccg ggctaaatgc agtgcgcctg attaatgagc caacggcagc cgcgatggcg
    35161 tatggcctgc atacgcagca aaatacccgc tcgctggttt ttgatctcgg tggcggcacg
    35221 ttcgacgtta cggtgctcga gtacgccacg ccggtgattg aagttaacgc ctccgctggc
    35281 gataacttcc tcggtggcga agattttacc catatgctgg tcgatgaggt tttaaaacgc
    35341 gcggaagttg ccaaaaccac gcttaacgat agcgaactgg cagccttgta cgcctgcgtg
    35401 gaagcggcaa aatgtagcaa tcaatcgcca atgcacattc gctggcagta ccaggatgaa
    35461 atgcggaaat gcgaatttta cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat
    35521 cgcttgcgag tgccgattga acaggcgttg cgcgatgcac gtctgaagcc gagtcaaatc
    35581 gacagtctgg tgctggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgcagtg
    35641 cgtctgtttg gcaaattacc gtatcaaagt tacgatccga gcaccattgt cgcgctgggc
    35701 gcagcaatcc aggccgcctg ccgcttacgc aatgaagata ttgaagaagt gatcctcact
    35761 gatatttgcc cttactcgtt gggtgttgaa gttaaccgtc agggcgtatc cggcattttc
    35821 tcgccgatta ttgaacgaaa caccacagta ccggtgtcgc gtgtagaaac ttattcaacc
    35881 atgcacccgg aacaggattc aattacggtt aacgtctatc agggagaaaa ccacaaagtt
    35941 aaaaataaca ttcttgtgga atccttcgat gtgccgttga agaaaaccgg gacttatcag
    36001 tcgattgata ttcgctttag ttatgatatc aacggcttgc ttgaagttga cgtgcttctg
    36061 gaagacggca gcgttaagtc cagagtgatt aatcacagtc cggtaacatt gagcgcgcag
    36121 cagattgaag agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc
    36181 atcaatcgca cctttaaagc caaactggaa gagttgtggg cgcgggcgct gggtgacgag
    36241 cgagaagaga ttggtcgggt gatcaccgat tttgatgcgg cgttgcagtc aaacgatatg
    36301 gcccgcgtgg atgaagttcg gcggcgggcg agcgattatt tagccattga gatcccataa
    36361 acacggctac cggatgcggc gtaaacgctg catccggcat cggcgcaaaa aacacatcgt
    36421 caactaaaca acatcgcgat tttgccgaga gcataaccta tcaccagcac cgttaaactg
    36481 attttgataa tgctggtaat gctctgtggt ttttcatcct catcatttat ttcttcatgg
    36541 ttttcaggtg ttggctcatc gacatcttca tcctccggct cgagcttcac tttcgtcaac
    36601 agcattcggg tgataatggt ctctaatgga tgtgcctctc cgcgttccag ccgcgcgtcc
    36661 agacgggcca gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga
    36721 tacaaatcca gtaacggtga atcttgctct gtctcgctac cgactctcga tttccaccac
    36781 gcccatgctt tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat
    36841 tttccgctat gtaaaatctc cgcccataac agccgcgtcc gggcgctctc accaggaaca
    36901 tatttcaacg gcttaccttg cgcatctacc atgctccgcg cttcgataac catcactaat
    36961 aacgcgaaat aatccgggca atgacgcttg ctccactgca acaacaaatt atcggcttgt
    37021 gtagatgggt aactttcccg gtacgcgcag agatcggcaa gcaggctttc cccctcgccg
    37081 cagtacaacc gttgtgcgca ctggtaataa cgggcatctt cgctttctgg ttcttccgct
    37141 tgccactgtt gagcgacagg aatgagttcc gccatacctg tttgcaccga gctgtaccag
    37201 cgcaacagtt tgcgatgcaa cttcgcatca tcaggaatga accacggacc ttccatcgcc
    37261 aaaaacgcag tgacatagtc aggatggtaa cggcagcaac gttccagcga gaagtaatac
    37321 tcaaccgtct ggttctgcaa cgcaacggga tattccagaa ggctaaggaa atcgaaacaa
    37381 tcaccagcct gaatggcctc aagaaaagcc tccctttctt cctcatctat tccttcgcag
    37441 tcattaaacg aatgccagtt caaacgctct gccagcaaag aagcacaatt taatgaaaga
    37501 tatcgcgctt ctatagcgat tgcacacagc ggccagcgta attgatcgac atcctccatg
    37561 ttccaggtat ttaattgctg aataaatttc tgccaggcgg agggctgaaa acgatcattt
    37621 tctgaatcca gtaatgccct gaatacacgt agtatttcat gctcagtggc agcatctttt
    37681 tcttcatcat ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc
    37741 tggcgtaatg ctttaaaccc ttgtggatcg gtttcgggat gacataacgg taagcgagca
    37801 agataagcct ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa
    37861 catgttttca taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta
    37921 atggctgcca gggaagcgtg gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca
    37981 tccgactccc ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt
    38041 cttcgcactg agaagattgc cagtagagac gccctaacga ataggcagaa aaatagtgca
    38101 tccagctgtg atagtaatgg tgtgctcgta tatagacgcg cgattgcagc cataaactct
    38161 cgtcttccgt caaccacttg ttgagcacac caacccgtga taaaaaaccc atccgcacat
    38221 aatcccaggc tttgattccc ccttccccac aacacatagc ggtttgggca acgaattggg
    38281 tataaatcaa ccctctctcc gagccgccag caattaatgc tttccactct tcaggggaag
    38341 agcgatgcca tgtcagatat aagccagcta aatcattggc atgaccatca tcggtcatct
    38401 gataaatagt ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga
    38461 attcttttat cccggtatac atttttggcg agtcatgcgc gggcttgcaa ccctgattaa
    38521 gtatttccat gggcgtagaa agcgcaaaaa gaagatattg ttcttcct
//
LOCUS       DAPQZD010000027        38298 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPQZD010000027 DAPQZD010000000
VERSION     DAPQZD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.027"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            184..1839
                     /gene="aslA"
                     /locus_tag="RO454_001365"
     CDS             184..1839
                     /gene="aslA"
                     /locus_tag="RO454_001365"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDY0933222.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1930..3165)
                     /locus_tag="RO454_001366"
     CDS             complement(1930..3165)
                     /locus_tag="RO454_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDY0933223.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPELNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3312..3388)
                     /locus_tag="RO454_001367"
     tRNA            complement(3312..3388)
                     /locus_tag="RO454_001367"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Pro,seq:tgg)
     gene            complement(3431..3517)
                     /locus_tag="RO454_001368"
     tRNA            complement(3431..3517)
                     /locus_tag="RO454_001368"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3481..3483),aa:Leu,seq:cag)
     gene            complement(3538..3613)
                     /locus_tag="RO454_001369"
     tRNA            complement(3538..3613)
                     /locus_tag="RO454_001369"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3578..3580),aa:His,seq:gtg)
     gene            complement(3672..3748)
                     /locus_tag="RO454_001370"
     tRNA            complement(3672..3748)
                     /locus_tag="RO454_001370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3712..3714),aa:Arg,seq:ccg)
     gene            complement(3851..5236)
                     /locus_tag="RO454_001371"
     CDS             complement(3851..5236)
                     /locus_tag="RO454_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDY0933224.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5427..6167)
                     /gene="rffM"
                     /locus_tag="RO454_001372"
     CDS             complement(5427..6167)
                     /gene="rffM"
                     /locus_tag="RO454_001372"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDY0933225.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6170..7522)
                     /gene="wzyE"
                     /locus_tag="RO454_001373"
     CDS             complement(6170..7522)
                     /gene="wzyE"
                     /locus_tag="RO454_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDY0933226.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7519..8598)
                     /gene="rffT"
                     /locus_tag="RO454_001374"
                     /gene_synonym="wecF"
     CDS             complement(7519..8598)
                     /gene="rffT"
                     /locus_tag="RO454_001374"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDY0933227.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8595..9845)
                     /gene="wzxE"
                     /locus_tag="RO454_001375"
     CDS             complement(8595..9845)
                     /gene="wzxE"
                     /locus_tag="RO454_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDY0933228.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9847..10977)
                     /gene="rffA"
                     /locus_tag="RO454_001376"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9847..10977)
                     /gene="rffA"
                     /locus_tag="RO454_001376"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDY0933229.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10982..11656)
                     /gene="rffC"
                     /locus_tag="RO454_001377"
                     /gene_synonym="wecD"
     CDS             complement(10982..11656)
                     /gene="rffC"
                     /locus_tag="RO454_001377"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDY0933230.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11634..12515)
                     /gene="rfbA"
                     /locus_tag="RO454_001378"
     CDS             complement(11634..12515)
                     /gene="rfbA"
                     /locus_tag="RO454_001378"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDY0933231.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12534..13601)
                     /gene="rffG"
                     /locus_tag="RO454_001379"
     CDS             complement(12534..13601)
                     /gene="rffG"
                     /locus_tag="RO454_001379"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDY0933232.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13601..14863)
                     /gene="wecC"
                     /locus_tag="RO454_001380"
     CDS             complement(13601..14863)
                     /gene="wecC"
                     /locus_tag="RO454_001380"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDY0933233.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14860..15990)
                     /gene="wecB"
                     /locus_tag="RO454_001381"
     CDS             complement(14860..15990)
                     /gene="wecB"
                     /locus_tag="RO454_001381"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDY0933234.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16046..17092)
                     /gene="wzzE"
                     /locus_tag="RO454_001382"
     CDS             complement(16046..17092)
                     /gene="wzzE"
                     /locus_tag="RO454_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDY0933235.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17104..18207)
                     /gene="wecA"
                     /locus_tag="RO454_001383"
     CDS             complement(17104..18207)
                     /gene="wecA"
                     /locus_tag="RO454_001383"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDY0933236.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18447..19706)
                     /gene="rho"
                     /locus_tag="RO454_001384"
     CDS             complement(18447..19706)
                     /gene="rho"
                     /locus_tag="RO454_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDY0933237.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19791..19892)
                     /gene="rhoL"
                     /locus_tag="RO454_001385"
     CDS             complement(19791..19892)
                     /gene="rhoL"
                     /locus_tag="RO454_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDY0933238.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20033..20362)
                     /gene="trxA"
                     /locus_tag="RO454_001386"
     CDS             complement(20033..20362)
                     /gene="trxA"
                     /locus_tag="RO454_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDY0933239.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20493..21758
                     /gene="rhlB"
                     /locus_tag="RO454_001387"
     CDS             20493..21758
                     /gene="rhlB"
                     /locus_tag="RO454_001387"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDY0933240.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21894..23378
                     /gene="gppA"
                     /locus_tag="RO454_001388"
     CDS             21894..23378
                     /gene="gppA"
                     /locus_tag="RO454_001388"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDY0933241.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPPQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFEQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFANRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23425..25446)
                     /gene="rep"
                     /locus_tag="RO454_001389"
     CDS             complement(23425..25446)
                     /gene="rep"
                     /locus_tag="RO454_001389"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDY0933242.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25533..25814
                     /gene="ppiC"
                     /locus_tag="RO454_001390"
     CDS             25533..25814
                     /gene="ppiC"
                     /locus_tag="RO454_001390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDY0933243.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25860..27335)
                     /gene="ilvC"
                     /locus_tag="RO454_001391"
     CDS             complement(25860..27335)
                     /gene="ilvC"
                     /locus_tag="RO454_001391"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDY0933244.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27485..28375
                     /gene="ilvY"
                     /locus_tag="RO454_001392"
     CDS             27485..28375
                     /gene="ilvY"
                     /locus_tag="RO454_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDY0933245.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28372..29916)
                     /gene="ilvA"
                     /locus_tag="RO454_001393"
     CDS             complement(28372..29916)
                     /gene="ilvA"
                     /locus_tag="RO454_001393"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDY0933246.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29919..31769)
                     /gene="ilvD"
                     /locus_tag="RO454_001394"
     CDS             complement(29919..31769)
                     /gene="ilvD"
                     /locus_tag="RO454_001394"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDY0933247.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31834..32763)
                     /gene="ilvE"
                     /locus_tag="RO454_001395"
     CDS             complement(31834..32763)
                     /gene="ilvE"
                     /locus_tag="RO454_001395"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDY0933248.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32783..33046)
                     /gene="ilvM"
                     /locus_tag="RO454_001396"
     CDS             complement(32783..33046)
                     /gene="ilvM"
                     /locus_tag="RO454_001396"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDY0933249.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33043..34689)
                     /locus_tag="RO454_001397"
     CDS             complement(33043..34689)
                     /locus_tag="RO454_001397"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDY0933250.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34692..34742)
                     /locus_tag="RO454_001398"
     CDS             complement(34692..34742)
                     /locus_tag="RO454_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933251.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34829..34927)
                     /gene="ilvL"
                     /locus_tag="RO454_001399"
     CDS             complement(34829..34927)
                     /gene="ilvL"
                     /locus_tag="RO454_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDY0933252.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35280..36800
                     /locus_tag="RO454_001400"
     CDS             35280..36800
                     /locus_tag="RO454_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDY0933253.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36825..37163)
                     /gene="maoP"
                     /locus_tag="RO454_001401"
     CDS             complement(36825..37163)
                     /gene="maoP"
                     /locus_tag="RO454_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDY0933254.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37282..38121
                     /gene="hdfR"
                     /locus_tag="RO454_001402"
     CDS             37282..38121
                     /gene="hdfR"
                     /locus_tag="RO454_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDY0933255.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38217..38292)
                     /locus_tag="RO454_001403"
     tRNA            complement(38217..38292)
                     /locus_tag="RO454_001403"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38257..38259),aa:Trp,seq:cca)
BASE COUNT         9365 a        10459 c         9659 g         8815 t
ORIGIN      
        1 tatcacctgc gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg
       61 gataaataaa taatttattt ttatcaccat tatttatgca atcatcctgt cagggagagg
      121 gatctcaatt atcaatgctt aattacgtca tcattttgac gacatgaaac cggagcattt
      181 tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc agctcttcct
      241 ctcatagcca gcgcagcaga taccccgtca accgccaccg cacgcaaagg ctttgccgga
      301 tacgatcacc caaaccagta tctggttaaa ccggcgacca ctattgccga caacatgatg
      361 ccggtgatgc agcatcctgc acaagacaaa gaaacccagc agaaactggc ggaacttgag
      421 aaaaaaaccg gtaagaaacc gaacgtggtt gttttcttgc tggatgatgt gggctggatg
      481 gacgttggct ttaacggcgg cggcgtggcg gtgggtaacc ctacaccaga tatcgacgcc
      541 gttgccagcc aggggctgat tttaacttcg gcgtattctc aaccgagttc ttccccaacc
      601 cgcgccacga ttctcaccgg acaatactcc atccaccacg gcattctgat gccgccaatg
      661 tacgggcaac cgggcggtct gcaagggtta accacgctgc cacagttgct gcacgatcag
      721 ggctacgtca cccaggctat cgggaaatgg catatggggg aaaacaaaga gtcgcagccg
      781 cagaacgttg gctttgatga tttccgcggc tttaactcgg tatctgatat gtataccgaa
      841 tggcgtgatg ttcatgtcaa cccggaagtc gccctgagcc cggatcgttc tgaatacatc
      901 caaaaactgc cgtttagcaa agatgacgtt catgccgtgc gcggcggtga acaggaagcc
      961 attgccgaca ttacgccgaa atatatggaa gatctggatc aacgctggat ggaatatggc
     1021 gttaagttcc tcgacaagat ggcgaagagt gataagcctt tcttcctcta ttacggcact
     1081 cgcggctgtc acttcgataa ctacccgaac gccaaatatg cgggtagctc tccggcgcgc
     1141 acctcttacg gcgactgcat ggtggagatg aacgatatct tcgccaatct gtataaagca
     1201 ctggagaaaa acggtcagct tgataacacg ctgattgtgt tcacctctga taacggtccg
     1261 gaagccgaag ttccgccgca cggtcgcacg ccgttccgcg gtgcgaaagg ttctacatgg
     1321 gaaggcggcg ttcgtgtgcc aaccttcgtt tactggaaag gcatgatcca accgcgtaaa
     1381 tctgacggca ttgtcgatct ggcggatctc ttccctacgg cgctggatct ggcggggcat
     1441 cctggggcga aagtagcgaa tttagtaccg aaaaccacct ttatcgatgg tgtggaccag
     1501 acatctttct tcctgggaac aaatggtcag tctaaccgta aggccgagca ttacttcctc
     1561 aacggtaaac tttctgctgt gcgtatggat gagttcaagt atcacgtcct gattcagcaa
     1621 ccttacgctt atacccagag cggatatcag ggtggattca ccggcacagt aatgcaaacg
     1681 gcgggatcgt cggtgtttaa cctgtacacc gatccgcagg aaagcgactc tatcggcgtg
     1741 cgccatatcc caatgggtgt accgctacag accgaaatgc atgcgtatat ggagatcctg
     1801 aaaaaatatc caccacgcgc gcagattaaa tctgattaag ccggcgctat cgctgagatc
     1861 tacctttgcc ggatgcgatg ctgacgcatc cgacaatcaa ccgcaggcgg ccacaaaatg
     1921 gctgcttttt tacttattca ccaccagcaa atgcgcctgc ataatgtcgc tggccggacg
     1981 accgtgcgcc agcaaatcaa ccattgcttt aagatatggc ggtagatggc ggaaataacg
     2041 ctgatacccg gcacacaaat aattcagctc gggtttgccg ctggcatcga gcataaagcg
     2101 gtgtttcggg cagcctcccc agcacgcttt taacacgttg caactgcgac actgtgccgg
     2161 taactgcttg aatttatctt caccaaatgc ctgctgttgc ggggaatcaa tcatttctgc
     2221 aatcatctgc tgaagcatat tccctagtcg atactgcgga taaacatagt gatcgcaggc
     2281 ataaacgtca ccgttgtgct caacaatcac cgagcgccca caggttggct gatgatggca
     2341 aaccgcgccc ggtgcgccga caaaattggc gaacgcccat tcgatattca ttacgaaaat
     2401 cttgccgacg tcgcgcttaa tccagtggtc gaaaatcgcc accagaaact caccgaactc
     2461 ttgcgggcac accgaccatt ccgtcagttc gccctgaata tcgccgggag catgtagctt
     2521 aagtccagca tgagcagccg tttcattagc caggcgctcg accaccggaa taaactggat
     2581 gaactcaaca cctgcatcac acagaaaatc atagacttgc aaaggttgta acgcactggt
     2641 gcgattgacg cagaccagca cgttatagtc gacatgatgt ttttgcagga gcgtcagggc
     2701 acgcatcacc agcttatgcg tgggtctgcc acctttggtc acgcgatatt gattgtggat
     2761 ctcagccgga ccatccagcg ataaccctac aagaaaatga ttttctgcca gaaatccaca
     2821 ccattcgtca tcgagcagca cgccgttagt ctggaagcta ttacttatct tcctgccagc
     2881 accatatttc gcctgtagcg ccacggcacg gcggtaaaac tccagcccga gtaacgttgg
     2941 ttcgccgccc tgccaggtaa aagccacttc gttttgggtt tcgctggcag cgatatagtg
     3001 acggacatac gcttccagcg tgtcatcgtc catatgcgtg actggctttt cgtggtaaag
     3061 ggattgtttt tcgagataaa aacagtagtc acagttcaga ttacaatcag aaccgctcgg
     3121 tttcgccatc acatgaaaag cacgcgttgg aacctgttgc tgcatggtcg ctccttgcct
     3181 gaagtcatcc ttcaataagt taagggcgaa atcataccgt ggcgcaatgc gttattacgg
     3241 gcgtttattg cgaagggaaa gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg
     3301 gggttcaaaa ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag
     3361 ttgcgctacc aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc
     3421 tttaaagtcg tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac
     3481 ctgaagctag cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg
     3541 ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact
     3601 gagctatagc caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg
     3661 ccgggactaa atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca
     3721 gcgctctatc cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac
     3781 ttcccacccc tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc
     3841 gttttgctgc ttattcctcc agtttatgcg ctttaccgtg gcgattaagc ccaaaaactt
     3901 tataaatcgc cgtcaccgtc agcataaaga tgatgccgac aaacagcgac atacgcgtat
     3961 cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg
     4021 cccaagggaa caggattgag cggaacggat ggctggcaat tgccgcttta tgcgcacgac
     4081 gaaaacgcag ctggctaatc aatatcacaa accatggcac catccccgga agcacgctgg
     4141 cactgtagac atagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc
     4201 caattagcag aatagcaata gataccgcca cacccgcaac cggtacgccg tgacgggaaa
     4261 ctttcgccat cgccgccggt aactgacggt ttttcgccag tgcatagagc atacgtccgc
     4321 aactgtacat gccactgtta cagccagaaa gcgctgccgt cagcaccaca aagttaataa
     4381 tgcccgccgc agcggtaatg ccgattttgg cgaaagtcag tacgaacggg ctgccgttgc
     4441 tgcctatttc attccacggg aagatggtaa cgataacgaa aatcgcgcct acgtagaaaa
     4501 tcaggattcg ccacagcacc ttgcctacag cgctacgcag cgtcacctgc ggattcttcg
     4561 cttcaccggc agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca
     4621 atgcggtcag gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc
     4681 taaaaccaat cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga
     4741 caatcatcac gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt
     4801 acaacctcac cgcagccaga ttcgccaacg ccaccagcgc caccgcgatc aatgcgggta
     4861 tccactgcgc catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg
     4921 agatccccac cgccatccac ataaaccagt aagaccaggc ggtgagatag ccaaagaacg
     4981 ggctcatata acgatgcgcg taaacggcga acgaaccggt aaccggttcg aggaacaaca
     5041 tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca
     5101 atacggatgg cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa
     5161 tggtgccccc cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg
     5221 gtttgttatc tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta
     5281 tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa
     5341 agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg gagtgaccac
     5401 ttcgtcgctt tacaaagaga ggaaaatcag aggttgccgg tatagtgcca gcgtaaataa
     5461 cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac
     5521 tccagcccca gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg
     5581 taggtcccgc caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg
     5641 aagatctcct gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga
     5701 atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat aaccatcctg gctgccaacg
     5761 atattcacat tccactggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta
     5821 ccgcccacaa gaaataccgg tgtcccttct ttgcctgcgc gcgccatcag ctcttcccag
     5881 agatcggccc cagcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt
     5941 acaacgctta tgccatccgc atatttaaat tcggcagcgt taattagctc ccggacctcg
     6001 gcgttatctt caatggtcag cattttttcg gcattaatgg caaccagcgt tccttgctta
     6061 agctgcccgt cagcgaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac
     6121 tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt tattcattat tatccttcaa
     6181 cctgcgtccg gagcgatgat tttgttcgtt tatgaatgag tccggcgctt tcaaaaagcc
     6241 agtacaacag ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagaccacgc
     6301 gtgagacaaa cgaatccagc ccttcacgcg ccagcacgat catattgaag atcgccccga
     6361 agcagaaact gtgcaatatc gcagccttat agcgattagt ctcgcggttg cccagctcat
     6421 acagccagtc gaaccatttg atgatcagtc caacaacaat cgccccgagc gggatgaaca
     6481 acgcgccgcc catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt
     6541 ggttattcag cacttcccag gtaaagtagt tggcggagtt cagcaccata ctcgggcgac
     6601 ccggccacag ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct
     6661 ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggtgaaaagg
     6721 tgtcgcgagt gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat
     6781 agcgttttaa cgccagccag aacatgccca caatccccag cacgcccgcc gccgccagca
     6841 tccacaacga aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga
     6901 tgatattggc gcgagtgccg ccgacaatca tataagtcag caagccaaaa gcgaccgtgc
     6961 tgacgaggaa aaacagccac gctttgctgt cctggcgcag aaagtagacc accagcatcg
     7021 ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggagaaga
     7081 tctgactgta ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga
     7141 cgcttaccag cgcgatgccc atcaggatca cccacgtgag attggtttcc acgcggttca
     7201 tggtaaacag cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg
     7261 tgacgtagta aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg
     7321 gcggcgcggc accaacatca aagcgaaaaa ccagcacgct ggtcagcggg aagccgaaga
     7381 aaaaggtgag caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa
     7441 actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag cagccagaca acaaacaggc
     7501 cactgaattg cagcagactc atgcgacctc cccggcggca atcgccaacg cccgctgcca
     7561 gccttgcaga tagttcgggc taaagaaggc aatggtgttt ttatccaccg acaccaactg
     7621 gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcaccggcaa
     7681 atgctgttcc gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc
     7741 cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc
     7801 aagatcgcac tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcagagt
     7861 ttgtagattt tcttcgctga ataactccag ccccgcctga cgaacttcct cgatgtacgc
     7921 ttcgttatta ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg
     7981 ataaacggcg cgcaaggcag caatatgttc attgctgcgg tcaccggagt tccccaccag
     8041 aatggtcatt ttcccttcac gttgccgatc attcgccatc atattgagtg aagggtccat
     8101 ccgcgtcggg aagtacagca gttctccccg cacctttgga tgcgttttgg caaaaaagct
     8161 caaatcaccg cgggtggcaa atacacagcc gactctcttt tgcgccaggc gacgtagcgg
     8221 gtaaaaaagc ttatatctca agccactgga aagctcgtac aggtctgccc cccagatatg
     8281 ccagtaaaac tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt
     8341 gaactgaccg tggaagaaaa aacgctgctg acggttagct tttgctttcg cgatgaccgc
     8401 ttccgccagc gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact
     8461 taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag
     8521 cgcgtcattg aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac
     8581 gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa
     8641 aaatagacga tataggttgc catataggcc tgcgccgcgc ccagtgcacc atgcgcaggg
     8701 attagccagt gggcaaatac catcaataaa gtgaactggc tgacttccgc cagaatataa
     8761 aaccgcagtg acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac
     8821 acatcaccca ccaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac
     8881 agcagccaga tagcaaaatc acgcagcaac cagacggtaa aactcgccgc cgccactgcc
     8941 ggcaagacga atttaagcga tttaaccact tcccgggtga tatcgcgctt ttccgttagc
     9001 cgcgacaacg tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca
     9061 tcggaaatac tgctcacccc ttgccagatc cccacctcat cccagctata ctgcgccgcc
     9121 agcagtttac gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg
     9181 agcgtaaatt tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt
     9241 aacgggatga caccacgttt gatcaacatg atggcggcag gaattaccac cagcgcggga
     9301 atcagcgcca gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac
     9361 gcgagaacgc caatcaagct gccaacaatc agagataacg cattacctgc ggcatcgcga
     9421 aagcctttca tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc
     9481 actaaacgca ccagcccctg atagtcggta ttaccaaaca atccctggct gattggcgca
     9541 gctgccagca caaaaaccag cgccatcagc gtagagaaac caagtaccat cgctgatgaa
     9601 gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt
     9661 acaccgttaa agatgccagc cccggcaagc acgccgagca cggtaatcaa ctggcggaaa
     9721 tttgccgcca gcccaagccc cgccggacca aatgacaccg ccagcaactt accgactagt
     9781 aacccggcac caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac
     9841 gacatatcag gagaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga
     9901 caggttgtag aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc
     9961 ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg
    10021 aaacaccgcc atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat
    10081 atcccgcagt ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg
    10141 cagctcgata cgcccatttt ttgccagagg cgccagtgca tcgtagtagt tttgccacag
    10201 cgccagacgc tgctgattga tacgatccgc tgcttccagt tgcgcccaca ggtacgctgc
    10261 ttgcagatcg gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg
    10321 accacggaag aactggctgc ggtttgtgcc tttttcacgg atgatctcgg ctcgttcgat
    10381 caacgcttta tcgttaatca gcgtcgcgcc gccttcacca cccgccgtgt agtttttggt
    10441 ttcatggaag ctaaagcagc caatatgacc aatggttccc agcgcacgcc ctttgtaagt
    10501 ggacatcacg ccctgagcgg catcttccac cacaaataga ttatgcttct tcgccaacgc
    10561 cataatagtg tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt
    10621 tttgtcggtg atcgccgctt caatcagcgt ttcgttgatg ttcatggtgt ccgggcgaac
    10681 atccacaaag acgattttcg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt
    10741 gtagctcggc atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc
    10801 cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag
    10861 ccactgctgg cagcgacggg taaaaccacc atcgccgcac agtttgccgc tacccattgc
    10921 cgactgcata tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg
    10981 atcacctgta taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt
    11041 taagcgcggc ggtgttgccc atctgggtcg ccacccgcaa agttgtttta ccgcgagcat
    11101 acgcccagtt tagcgccgtt tgcattagct cagcacctgc accgcgtcca gccagcaggc
    11161 caattcgcgc atctgtcgca ttgagttccc gtaaagagac ataaccgcga atatcgccgg
    11221 acgccgcacg taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc
    11281 actgtgcata aaagcgaccg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt
    11341 gcgcaaatgc ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta
    11401 ctgcaccgct atcactgaca ttgttcacgg gtagtgccaa atcaacttca ccttctatca
    11461 gggagaatcc cagctgttgc agagcatcca gttcacccgt atttgatgcc gcaattttgg
    11521 cctgcacccg tgaccacggc gctaaggcgt ctggcgtcag gagtgccgcg tcagggctaa
    11581 tacgcacaat ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac
    11641 tggcgcggac gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat
    11701 gaactggcag cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcaatctct
    11761 tccaggcagg caatcttaaa gccctggcgt ttttctactg tctgcacaaa agtgctggcc
    11821 tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa atccgcgccc gagcagttca
    11881 acggtcaggt tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca
    11941 cgctccgacg gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc
    12001 ccggtcaccg cccagtttga tttcggctgt tttggttttt cttccagcga gatagcgcgg
    12061 aaattgtcgt caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat
    12121 accgtcgccc cttccgtgcg cgccgcaaca tgacgcagtt tcggactgaa gccctgtccg
    12181 aagaagatgt tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg
    12241 atgataaagg cctgcgccag gccgtccgga ctgggctgtt cggcatattc cagctggata
    12301 ccgaactcac tgccgtcacc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg
    12361 atgatgagaa tttcgcggat accggccagc atcagcaccg acagcggata gtaaatcatt
    12421 ggcttatcgt aaatcggcaa cagttgctta gatacgccgc gcgtaatcgg atgcaatcgg
    12481 gtgccggaac cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct
    12541 ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca
    12601 ttagccagat accactgcac cgttttacgc attccacttt caaaggtttc ctgcggcagc
    12661 cagccaagtt cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga
    12721 cggtcagcga caaaggtgat caggtcacga tagtgcgcca cgccgtgcgg cttattcgga
    12781 gccagttctt ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg
    12841 tgaccaccaa tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg
    12901 cgggcgtgat cttcaacata cagccagtca cggatttgct gcccgttgcc atataccggc
    12961 agcggtttac ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg
    13021 taaggaccgt agttattcga gcagttggtg ataagcgtcg gcagaccata ggtccgcagc
    13081 caggcgcgca ccagatggtc gctgctggct tttgacgcgg aataagggct gctcggcgca
    13141 tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg
    13201 gtggagatat gatgaaaacg gaacgctgat tttttatctt ccgtcagcgc agtccagtaa
    13261 gcccgcgccg cttcaagcag tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc
    13321 gggccgtcaa tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc
    13381 tgatactcag tgaatacgcg cgccagttct gcccgatcgc agatatcaac tttctcaaag
    13441 gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc
    13501 gtatcgacca ccaccaccgc gtcgctcgtt tcgttgatga tataacgcac cagcgccgag
    13561 ccaataaacc cggcaccacc tgttatcaga atctttctca tcagcgccag actcctttgg
    13621 catcgacgac atactgctga tggacgttgt cgccattgat aactttgaac tgactatgat
    13681 cgaccagcat caccagcaca tctgccgttg ccagcgcctc gtcaagctgc gccagagtac
    13741 aaagcccggt cagtttcttc ggcaactggt ggatgttagg ctcaacaacc agcgtttcgc
    13801 cgctgtgcca ctgggcgatc agttcagcga tttccattgc cggactttcg cgcaggtcat
    13861 caatattcgg tttaaacgcc agaccaaagc aggcgatttt cagttcattg gcgcgtttat
    13921 cggtagcagc cagacaatca gccaccgccg ctttcacctg atcgataacc cagaacggtt
    13981 tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca
    14041 cgataaacca cggatcaaca gcgatgcagt gaccgcccac gccagggcct ggctgaagaa
    14101 tattgacgcg agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat
    14161 cggcacaaat cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt
    14221 cggtgagctt acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga
    14281 aaattttgta cagttcgctg gcgcgggccg aacaaaccgg cgtcatacca ccaatcacgc
    14341 gatcgttttt aatcagctcg accataacct ggcccggtaa tacgcgttcc gggcagtaag
    14401 caatgttgac gtccgccaac tcgcccacct gctgtgggaa agtgagatct ggacgcatct
    14461 ctgctaacca ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca
    14521 ctaacgcgcc ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca
    14581 tatctggctc atgatcgcct ttaaacggtg tgggtacagc aatcaaccag gcatccgctt
    14641 caactggcgt cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg
    14701 ccaaatcagg ctcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt
    14761 tgatatcgac accaatcacc tgtttttgcc ttgaggcaaa cgcagctgct gtaggcagcc
    14821 cgatgtaacc cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt
    14881 ttttaacgct tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg
    14941 gctcatagct tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg
    15001 ctgcttatcc gtgccgacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt
    15061 ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc
    15121 tgagtcggtc aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg
    15181 gggatcgatc agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac
    15241 gttcgggttg agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc
    15301 tgccagcgca tggcagattt cttcaaagcc acgaccgaag ctctcacgcc tgtgaccggt
    15361 caccagaatc atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg
    15421 cagcgtatcg ctgctcatca cctggtcacg cacccataac agtgcatcaa tgactgtatt
    15481 accagtaatg aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt
    15541 ttcggttgga gaaaaatgat acatcgccag atgcccggtc aatgtacggt tagcctcttc
    15601 cggccacggc gaatagagat cgcccgtgcg cagaccagcc tcaacgtgac caacaggaat
    15661 acgctgataa aacgccgcca ggctggttgc cagcgtcgtc gtagtatcgc cgtgaaccag
    15721 cacgacatct ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt
    15781 tatctctgta aggccttgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga
    15841 aaagagtttc agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc
    15901 ctcaaaaaaa ggatcttttg ccaacgcatg caccaacggt gccatcttga tggcttccgg
    15961 gcgcgtacca aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc
    16021 ctttgcgctc accgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag
    16081 ccccgatcag tcccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat
    16141 cgcgttttac cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca
    16201 gggttggacc aacattcagg gtgtttaaca tggcccgatt ttgatcatag tcgagatcaa
    16261 aggccggacc gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca
    16321 ggaacatttc tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat
    16381 tatgctgctc agcaattttc agcgcctgtt caatactgtt catccggcgg tcatagatgg
    16441 ctttcgccac ctcttcctga cgcttcacct gagctttcat ctggatagtg cgtgccgccc
    16501 atgcgccttt cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat
    16561 actgacgtaa caggttatta gcgtcaggcg cggtttcggc aataagcttc acgctgtcat
    16621 tgaccgcgcg ggtaaagtca ccgggaataa acacgatgtt gttaatcatt tcatccagca
    16681 acgccgcatc ggctttgctg ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc
    16741 agaactctct gcgggtatcc cacgaggcca gttgcataac aaactcttta taagcttcgt
    16801 ccatgaccga tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa
    16861 attgctgctg tgagtagtac ccgcccagca tattcaccgt tggacgatcg gtaatcgccg
    16921 tcgcgctcca ctcctgacga gcaaaaaaag tatacgccag cgcgattaac gcaaacgcca
    16981 gccccatgcc aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat
    17041 ccagttcatt ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct
    17101 catttatttg gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg
    17161 cttaataaag cgagcaactt tccaggcacg cttgatgcaa tatccataga ggaagaatgc
    17221 tagcaaaaag agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac
    17281 gccaatggaa gcgagcaatg ctgcggcaag ggtaatcagc acaaacgcct ggcgggaagt
    17341 aaacccggca cgcatgatca aatggtgaat atgctgacgg tctggagaga atgggctcat
    17401 gcctttacgc aggcgacggt acataatcgc caccatatcc attagcggaa tggcgataat
    17461 ccacaaagcg gtaaccgggc tgatgggatg agttttgccc tgggtcgttt cgagcaggat
    17521 ccagataacg gtaaaaccaa tcagcgtact gcccgcatcc cccataaaga ctttgtagcg
    17581 gcgacccagg ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa
    17641 gcaccagatt gcgagactgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa
    17701 cgagacgcag gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt
    17761 aatggccgcc cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc
    17821 ccaggagcca aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac
    17881 aatgccaaca gcggcctgta tggtggctcg gatttttacg ctgatatcaa aacggtcatc
    17941 cagcgcgcca atgaaaacaa gcacaccagc acaagcgaga tagagagatg catgcggaat
    18001 atagtagtcg acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac
    18061 gagaggtatc aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt
    18121 ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag
    18181 atcagtactc actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa
    18241 gtataaccac gaagaccttt attcagaaag gaaagtccgt tccgaaccta ccagcactat
    18301 gcgaaaatta accacttgat atgcaaattg cataacacac cagcaaattc caggaatttt
    18361 cctaacatcg ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca
    18421 cgtggcgttt ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca
    18481 ttggtcttgg tcattgccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc
    18541 gggtgaatga ttttgcgcag gatccacatt ttctgcagtt cttcctgagt cgtgagcagc
    18601 tcttctttac gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg
    18661 atcttacgag agaggtgcag ttccatgttg cctgtacctt taaactcttc gtagataact
    18721 tcgtccattt tagaaccggt atcgataagc gccgtcgcga taatggtcag gctgccgccc
    18781 tcttccacgt tacgcgccgc accgaagaag cgtttcggac gatgcagggc gttggcgtcc
    18841 acaccaccgg tcaacacttt acctgacgcc ggaacaacgg tgttgtaagc gcgcgccaga
    18901 cgagtgatgg agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttcgcc
    18961 ttctcgatca ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa
    19021 gcaacaactt caccttttac cagtcgctgc atctcggtta cttcttccgg acgttcgtcg
    19081 atcagcagaa ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc
    19141 tgcagcagca tggttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga
    19201 ccgataggtg atgccagatc cagtacgcga gcggttaaat cttccgtaga accgttacca
    19261 cgttccatac gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg
    19321 ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag
    19381 cgttcacctt ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg
    19441 aaacggcgga tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg
    19501 tctgcggaac ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag
    19561 atatcttcgc cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc
    19621 atacgagcca ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc
    19681 gtattcttta attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc
    19741 gaacttaatg ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc
    19801 atgtcttttc gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg
    19861 agtgacgagc cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat
    19921 tacaagattc aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac
    19981 tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca
    20041 ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcgcccact ttggttgccg
    20101 ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg
    20161 cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt
    20221 cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga
    20281 aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca
    20341 ggtgaataat tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc
    20401 gttgttggtg taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt
    20461 aaagcaatag taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga
    20521 agttttccga cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc
    20581 ataactgtac gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag
    20641 ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt
    20701 atcttctctc tcatcccgcg attgctgatc gcaaggtgaa tcagccgcgt gccttaatta
    20761 tggcaccgac gcgtgaactt gccgtgcaga tccacgccga cgcggaaccg ctggcggaag
    20821 ctaccggcct gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag
    20881 tgctggaaag cggcgttgac attctgattg gcaccacggg tcgtttaatt gactacgcca
    20941 agcagaacca cattaacctc ggtgccattc aggtggtggt gctggacgaa gccgatcgca
    21001 tgtacgatct gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa
    21061 accagcgcct caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt
    21121 tcgagcagat gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc
    21181 gtattaaaga agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc
    21241 tgatcgaaga agagtggcca gacagagcga ttattttcgc caacaccaaa caccgttgtg
    21301 aagagatctg gggccacctg gcggcagatg gtcatcgtgt cggtttattg acaggcgatg
    21361 tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc
    21421 tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc tgctgtgaca cacgtcttta
    21481 actacgattt acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg
    21541 ctggcgcaag cggtcattcc atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg
    21601 ctattgagac ctatattggt cactcaattc cggtaagtaa atacaatccg gacgcattga
    21661 tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg cacaggcaat ggtccgcgtc
    21721 gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaag aaaaaatgtc atactccaaa
    21781 tcattcaagc tgtatcaaga tggcaagaga atgaatcccg atgagtttac tgtcgtaagt
    21841 gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt
    21901 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    21961 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg
    22021 ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat
    22081 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc cccgcaaatt cgcgttgtcg
    22141 ctacggcgac attacgcctt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa
    22201 tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcgcgtctg atttatcagg
    22261 gcgttgctca caccactggc ggtgccgatc agcgcctggt ggtggatatc ggcggagcca
    22321 gtactgaact ggtaaccggc acgggtgcac aaaccacctc tttgttcagc ctgtcgatgg
    22381 gctgcgttac ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg
    22441 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc
    22501 acggctggaa agtgtgcgtt ggcgcttccg gcaccgtgca ggcgttacag gaaatcatga
    22561 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaactg aaacaacgcg
    22621 ccattcattg cggtcggctg gaagagctag agattgacgg gctgacgctg gaacgtgcgt
    22681 tagtgttccc gagtggtctg gcgatcctga tagccatttt taccgaactg aatattcagt
    22741 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggtatg ttgcatctta
    22801 ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg
    22861 atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttcttcgaa caggtggaaa
    22921 atgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    22981 aaatcggcct gagcgttgac ttcaaacaag cgccgcaaca cgctgcttat ctggtgcgta
    23041 acctggatct tcccggtttt acccccgcac agaaaaaact gctggcgacg ctactgctca
    23101 accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg
    23161 tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccaac cgtcgccgtg
    23221 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    23281 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgatcag gaaagccagt
    23341 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    23401 ctgacacatt ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    23461 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc
    23521 ctgttcccaa atcaaatcat cctgcggcag ctccagcagg aagcggctcg gctccgggcg
    23581 caccagttcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    23641 gcgggtaatg ccgacatagg ccagccgccg ctcctcatca atattgtctt catcgatgct
    23701 gctctggtgg ggcaaaaacc cttcttccat accaaccatg tagacatagg gaaactccag
    23761 ccctttcgac gcgtgcagag tcatcagttg cacctgatcc agttcttcct cgctctcgcc
    23821 gcgctccatc atgtcgcgca gagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    23881 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    23941 catgcgcatt tcggcagctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    24001 catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    24061 ttctgccagc cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    24121 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccattc
    24181 acctagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag
    24241 aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    24301 ttcaggacga gagaaaaacg acgtaccgcc agatattttg tacgggatgc ggttttgcat
    24361 caggaatttt tcaaataccc gcgactgatg gttgccgcga taaagaatgg cgtaatcttt
    24421 gtactgcgtt ttattgacga agtgatgggc gatcagctcg ccggtgacgc gctcggcttc
    24481 atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa
    24541 cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    24601 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    24661 gctcagcagc accagattct gcggacgtgc gccgcgccag gagtagatcg actggtcatc
    24721 gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg
    24781 gctggtgttg gtgtcctgat actcatccac cagcaggtag cgaattttgt tctgccagcg
    24841 ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa
    24901 gtcgagaacg ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    24961 acggtcccgc tcgccctttg cctctgccgc cgcctgcgcc ggtgttttga gatcgttctt
    25021 ccagttggag atggtcgaaa ttagctgttg caggagaact ttgtcatctt caatcagccc
    25081 ctcggtcaac tctttaagca aagcaagctg atcggtatcg tcaaacagcg aaaagttagc
    25141 cttcatcccc agcgccgcat actcgcgttt gatgatatcc agccccagcg tgtggaaggt
    25201 ggaaatcatc agcccacgcg cctctttgcg ccccagcgtc tgcccgacac gctctttcat
    25261 ctcgcgtgcc gccttattgg taaaggtcac agccgcgata tgtcgcgcct gataaccgca
    25321 accgcggatc agatgggcga ttttattggt gataacacga gttttaccgg aacccgcgcc
    25381 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    25441 acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag
    25501 ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata
    25561 tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagatcaag aatggtgccg
    25621 atttcggcaa gctggcgaag aaacactcca tttgcccgtc aggcaaacgc ggcggtgatt
    25681 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagtggtg ttttcttgcc
    25741 cggtgctgga accaaccgga ccgctgcata cccagttcgg ttaccacatc attaaggtgc
    25801 tataccgtaa ttaacagcaa ggccttctcc gggagaaggc cttgaactct gaatcgctat
    25861 taacccgcaa cagcaatgcg tttcatatct gtcatatagc cgcgcagttt cttacctacc
    25921 tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc
    25981 gcaccttccg gaatagcttt acccaggtcg cccggttgca gctctgccat aaacggtttc
    26041 agcagtggca cacaagcgta agagaacaga tagttaccgt actcggcagt atcggagata
    26101 accacgttca tttcgtacag acgcttacgg gcgatggtgt tggcaatcag cggcagttcg
    26161 tgcagtgatt catagtaagc agattcttcg atgatgccag aatcaaccat ggtttcaaac
    26221 gccagttcaa cgcctgcttt taccatcgcg atcatcagta cgcctttatc gaagtactcc
    26281 tgttcgccga ttttgccttc atactgcggc gcggtttcga atgcggtttt gccagtctct
    26341 tcacgccagg tcagcagttt cttatcgtcg ttggcccagt cagccatcat gccggaggag
    26401 aattcgccgg agatgatgtc gtccatatgt ttctggaaca gcggcgccat gatctctttc
    26461 agttgctcag agagcgcgta agcacgcagt ttcgccgggt tagaaagacg gtccatcatc
    26521 agggtgatgc cgccctgttt cagcgcttcg gtgatggttt cccaaccgaa ctgaatcagt
    26581 ttttctgcgt atgccggatc ggtgccttct tccaccagct tgtcgaagca cagcagagag
    26641 ccagcctgca acataccgca caggatggtt tgctcgccca tcaggtcaga tttcacttcc
    26701 gcaacgaaag aggattccag aacgcccgca cggtgaccgc cggttgcagc cgcccaggct
    26761 ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc
    26821 ggtacgccga atccacgttt gtactcttca cgtacttcgg tgccagggca tttcggcgca
    26881 accattacga cggtgatgtc tttacggatc tgctcaccca cttctacgat attgaaacca
    26941 tgagagtagc ccagcgccgc gccgtctttc atcagcggct gaacggaacg cactacgtca
    27001 gagtgctgct tgtctggcgt caggttaacc accagatccg cctgcgggat cagttcttcg
    27061 taagtgccaa ctttgaagcc gttttcggtt gctttacgcc aggatgcgcg cttctcggca
    27121 atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat gttcaggccc
    27181 tggttcagac cctgtgcacc acagccgacg atgactactt ttttaccctg aagatagctc
    27241 gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccagctgc
    27301 tggcgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt
    27361 cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg
    27421 gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat taagggctgc
    27481 gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg
    27541 ccgcagcgcg cgggcaatgc acgttagccc gtccacgctc tctcggcaga ttcagcgcct
    27601 ggaagacgat ctcggtcagc cgctgtttgt gcgcgataac cgcacggtaa cgctaaccga
    27661 agcaggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc aacagctgcg
    27721 ccacaccatc gatcagcaag gaccgtcgct ctctggcgaa ttacatatct tctgctcggt
    27781 gaccgctgcc tacagccatc tgccgccgat tctcgatcgc ttccgcgcgg aacacccgtc
    27841 ggtggagatt aaactcacga ctggtgatgc ggcagatgcg atggaaaagg tcgtcactgg
    27901 cgaagcggat ctggcgattg ccgggaaacc ggagactctg cccggcgcag tggcgttttc
    27961 gatgctggag aatctggcag tagtgctgat tgcccccgcg ctaccctgcc cggtgcgtaa
    28021 ccaagtttcg gcagataagc cggactggtc tacagtgccg tttatcatgg ccgatcaggg
    28081 accagtacgc cggcgcattg agttgtggtt tcgacgcaac aaaatcagca acccgatgat
    28141 ttacgccacg gtcggcgggc atgaagcgat ggtgtcgatg gtggcgctcg gctgtggcgt
    28201 agccttgttg ccggaagtgg tgctggaaaa cagcccggaa ccggtgcgta accgcgtgat
    28261 gattttagag cgcagtgatg agaaaacgcc gtttgaactg ggcgtgtgtg tgcagaaaaa
    28321 gcggctacat gagccgctga ttgaggcatt ctggaagatt ttgccgaacc attaacccgc
    28381 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtaac ccaactcatt
    28441 caaccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc
    28501 gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg tacccagcgt
    28561 gttgaggaag cgcaacagcg cgcccggcga ttccgggaat tcgaagctgt agaggcgttc
    28621 ctgcaacgga tgcgacggac gcccgccgac catatagcgc acatgcagct tcgccatttc
    28681 gtcgtcggag agatccacta cgctgtagcc gccatcgttg agcatctgca aaatttcttt
    28741 gcgctcttcg aggccacggc tcaggcgcac accgacaaag atgcaggcgt ttttggcatc
    28801 ggcaaaacgg tagttgaact cggtgaccga gcgcccgcca agcagctggc agaacttcag
    28861 gaagctgccc ttctcttccg ggatggttac cgccagcaac gcttcacgct gttcgcccag
    28921 ttcgcaacgt tccgagacgt agcgcaggcc gtgaaagttc acgttagcac cagaaagaat
    28981 atgcgccagc cgctcaccgc gaatgttgtg ctgggcgata tattttttca ttcccgccag
    29041 tgccagcgcg ccagagggtt ccgctaccgc gcgcacatct tcgaacaggt ctttcatcgc
    29101 cgcacatatg gcatcgctat cgacggtgat gatgtcgtcg agatactcct ggcataaacg
    29161 gaaggtttcg tcgccgatgc gtttcaccgc aacaccttcg gcaaacagcc ccacgcgtgg
    29221 cagatcaacc ggatgacctg catccagcgc cgctttcagg caggcggagt cttccgcttc
    29281 cacggcaatc actttgattt gcggcatcag ttgtttgatc agtaccgcca cgcccgccgc
    29341 cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct gctggagcag
    29401 ttccagcgcc agcgtgcctt gcccggcgat caccatcgga tggtcgaacg gcggcaccca
    29461 ggtgaagccc tgctgctgtg acagttcgat cgctttcgct tttgcttcat caaagttcgc
    29521 gccgtgaaga agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga tatcggcggt
    29581 ggcggttggc atgacgatca gtgccttcac gcctaaccgt gcggaagaaa acgcgacgcc
    29641 ctgcgcgtgg ttacccgcag aagcggtgat cacgccgtgc gctttctgtt cttccgtcag
    29701 gcccgccatc atggcgtatg cgccgcgcag cttaaagcta tgaactggct gacggtcttc
    29761 gcgtttcacc agaatcacgt tatcgagacg cgacgagagt ttttccattt tttgtagcgg
    29821 cgtgacctgc gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat attcggcgcc
    29881 ttccggggcg ccggagagag gttgtgagtc tgccatcatt aaccccccag tttcgattta
    29941 tcacgcaccg cgcctttgtc ggcgctggtt gccaggctgg cgtaggcacg cagcgcaaag
    30001 gaaacctgac gttcacgatt tttcggtgtc caggctttgt tgccacgagc ttcctgcgct
    30061 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcggaatg
    30121 tcgatagcga tcaagtcacc atcttcaatc aggccaatgc tgccgccact tgccgcttcc
    30181 ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc gtcggtgatc
    30241 agcgcacagg ctttaccgag acccattgat ttcaggaaac tggttgggta gagcatttcc
    30301 tgcatccccg gaccgccttt cgggccttca tagcgaatta ctaccacatc tccggcgaca
    30361 actttaccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtagac tttcgccggg
    30421 ccagtgaatt tgaggatgct gtcatcgacg cctgcggttt taacgatgca gccgttttct
    30481 gcgaaattac cgtagagtac cgccaggccg ccgtctttgc tgtaagcgtg ttccagcgag
    30541 cggatacagc cattggagcg atcgtcgtcc agcgtatccc agcggcaatc ctgcgagaat
    30601 gcctgtgtag tacgaatgcc cgccgggcca gcgcggaaca tattttttac cgcatcatcc
    30661 tgggtcacaa tgatgtcgta ttgttccagc gtttgcggca acgtcaggcc aagtacattt
    30721 ttcacatcac ggttcagtaa ccccgcacga tccagctcgc cgagaatacc gataacacca
    30781 ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcgac tttacacagc
    30841 tgcggtactt tgcgggagag cttatcgata tcactcatgg tgaagtcgat ttccgcttcc
    30901 tgcgccgccg ccagcaggtg aagaacggtg ttggtagagc cgcccatcgc gatatccagc
    30961 gtcatggcgt tttcaaacgc cgccttactg gcgatgttac gcggcagtgc gctttcatca
    31021 tcctgttcgt agtaacgttt ggtcaattca acaatgcgtt taccagcatt gaggaacagt
    31081 tgcttgcgat cagcgtgggt tgccagcagc gagccgttgc ccggctgcga caggcccagc
    31141 gcttcggtca ggcagttcat tgagttagca gtaaacatcc cggagcagga accgcaggtt
    31201 gggcaggcgg atcgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcgccc
    31261 tggatcatcg catcaaccag atcgagctta atgatctgat cggacagttt ggttttcccg
    31321 gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc
    31381 agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtgg
    31441 gcgttgacca tatattcaac ggaatcagcg atcagttcgc gagatggcag tgaataaagc
    31501 atgcccccgt ggcccatagc gatcccatca tccaccgcaa tggtgttgaa ctctttggca
    31561 acgccgcccg ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatgaacg
    31621 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt accgaaatcg
    31681 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    31741 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    31801 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    31861 ccatttatct tcggtctcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt
    31921 aaccgggccg caacggcctt caccaacctg aataccatct acactgcgca ctggcgtgat
    31981 ttctgccgcc gtaccggaca taaagacttc atccgccagg tacagggatt cgcgcgacag
    32041 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt
    32101 aatacccggc agcgcggagg aggtgaacgg tggagtaaac agcacaccgt ctttcacttc
    32161 aaacaggttt tcgcctgcgc cttcagagat ataaccattc acatccaacg cgataccttc
    32221 ctgataaccg tggcggcgcg cttcgctacc caccaacagg gaagagaggt agttaccacc
    32281 agctttcgct gcggttggga tggtgtttgg tgcagcacgg ttccaggagg aaaccatcgc
    32341 atcgatcccc tgctccagcg cttctgcgcc cagatacgcc ccccacggga aagcggcgat
    32401 aatcacatcg gttgagtatc ccgctggcgg gtttacgccc atgccaacat caccgacgaa
    32461 aatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cgcgacaagc
    32521 ttccatcagc tcatcaatgc tctgcgaaac cgggaaacga tagattttgg cggagtcatg
    32581 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg agtcgtagca
    32641 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    32701 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    32761 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    32821 agatagcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    32881 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcagcca
    32941 tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    33001 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    33061 catttcagaa ttactggcac caggcggcac cagcggccag acgttctcaa gttcgtcgat
    33121 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    33181 ctggtcttta tgggtgatgt gttggccagg gatgccgaag gcgctggcta acatgaggaa
    33241 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tggaaaaaca gttgctgcca
    33301 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    33361 gcgttttacg gtgcccagct cttgcacatt catcataaaa gagccgtcac cggagataca
    33421 gacgacagta tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta aaccgaaacc
    33481 catggtgcct aagccgctgg aggtgatgaa gttttccggg cgagtgtgga cgatgtgctg
    33541 ggcagcccac atctggtgct gccccacatc tgtggtcacg atgcaatccg caggtttacg
    33601 atccgacagt tgttttaaca acaacggcgc gtagatagcg tcaccaggat ggtcgtaacg
    33661 ccaggcatgt tcatcacgca gttgcgcgca gtgttgctgc cagtcatcga tatttaacgg
    33721 ctgctgtaat gctggtaaca gagcatttaa atcaccctgt aatgccacat gtgcctgacg
    33781 cagcttgttc atttctgccg gatcgatatc catatggata acgctggcgt gtggtgcgaa
    33841 ggtgttcagt ttgccggtca cccggtcatc aaaacgtgcg cccacggcta tcagtagatc
    33901 gcactcctgc actgcgaagt tcgccgcttt ggtgccgtgc atccccagca tgcccagata
    33961 gtacggataa tctgcttcta ctgcgcccag ccctttcaac gtacaggtgg caggcatttt
    34021 tgtggcagcg agaaattcac gcaaagccga aactgcctgc gccatgccca cgccaccgcc
    34081 aacgtacagc atcggttttt gcgcttttgc cagcatctga cgcgcttgct cgacttcggc
    34141 atgtgggaaa gtcacttcgt tttcaacagt ggtgaaccac ggttccagat cgccgctggc
    34201 taactggata tcttttggaa tatcgaccaa aaccggacca ggacgaccag agctggcgac
    34261 gtcaaacgct tcggccatga tacgcggtaa ctcttccagt gattgcacca ggaaactgtg
    34321 cttggtgcag gctaacgaca accccagaat atccacctcc tgaaatgcgt cagtgccgat
    34381 aaacggtgcg gatacctgac cggtgatagc aacaacgggg atggaatcta acagcgcgtc
    34441 cgcaagcccg gttatcaggt tggttgcacc tgggccagac gtggcgatgc aaacgccagt
    34501 tttgccggta gcacgggcat aaccgatagc cgccattgct gcaccttgct cgtgtcggca
    34561 cagcaagtgt tccacgccac cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    34621 cggataaccg aaaacggtgt ttacaccctg tgcccgcaac gcatgtacca cccactgtgc
    34681 accattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc
    34741 attgtctgct cctcagttat gtttttaagg tcaaaaaaaa cccccggacc tttcggtgcg
    34801 ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt gcagccccgc
    34861 atggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact cgtagaaggg
    34921 ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt taccatagat
    34981 tttgcgaata gcacaagata ttttttggcc gatttttatg atgtctttaa taatctcaag
    35041 agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa aaattcaatt tcagtatgaa
    35101 tgttgttccc ttcctcgtag ttcatccttt gcccgctgca tttttgcgag catcattcca
    35161 ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac
    35221 gaatttgcgg gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggctta
    35281 tgtcactgtc aattgttcat acccgcgcag ccctgggagt aaatgcgccc ccaatcactg
    35341 ttgaggtaca tatcagtaaa ggtctaccag gcttaacgat ggtgggctta ccagaaacaa
    35401 cggtaaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc
    35461 cggcgaaaaa aatcaccatc aaccttgctc cagccgatct gccaaaagaa gggggacgat
    35521 atgatttgcc tatcgctatt gcgttgctgg cggcctcaga acagcttaca gccaataagt
    35581 tagatgaata tgaattagtc ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg
    35641 gcgcaatctc cagtgcaact gaagctatta agtcgggcag aaaaattatc gtcgcgaaag
    35701 ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc
    35761 aggctgtctg cgcgtttctg gaaggtaagc acgctctcga acacccgaaa ccaactaatg
    35821 cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag caaggaaagc
    35881 gaggactgga aattaccgcc gctggcgggc acaacctttt actgattgga ccgccgggaa
    35941 caggtaaaac aatgctcgcc agccgtatta atggcctttt gccagattta agcaatgaag
    36001 aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat
    36061 ggcggcaacg cccgttccgc tcacctcatc acagtgcgtc gttaactgcg atggtgggcg
    36121 gtggtgcaat tccagggcca ggtgaaattt cgctggcgca taacggcgtg ctttttcttg
    36181 atgagctacc tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg
    36241 ggcagatcca tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccagcttg
    36301 tcgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacac
    36361 cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc
    36421 tctcactgga gatcccatta ccgcctcccg gcattttgag taaaacggta gtgccgggag
    36481 aaaacagcac caccgttaaa caacgtgtaa tggccgccag agagcgccaa tttaagcggc
    36541 agaataaact gaacgcctgg ctggataatc cggaaatacg ccaattctgc aagcttgaga
    36601 gcgaagatgc gcagtggctg gaagaaacgc tgatccatct ggggttatcg attcgtgcct
    36661 ggcagcggtt attgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca
    36721 cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc
    36781 tgcaaaaact actgacataa aaaaagggca tttcgccctt tttattaatc gtcagaatcg
    36841 gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt
    36901 ggacgcttga tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggttca
    36961 cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg
    37021 ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg
    37081 gtgaagtcac catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta
    37141 gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg
    37201 gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca
    37261 aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc
    37321 cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccaatc agccgtgagc
    37381 tttcgaatca gacaactgga aaatcaactg ggtgtgaatc ttttcacccg ccacagaaac
    37441 aatatccgtt taaccgctgc cggagaaaag cttctgcctt atgcagaaac gctcatgagc
    37501 acctggcagg ccgcccgcaa ggaggtggcg catacctcac gacataatga gttttctatc
    37561 ggtgccagcg cctcgctgtg ggagtgtatg cttaatcagt ggctgggacg cttgtatcaa
    37621 aatcaggatg tccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg
    37681 gtaaaacagt tgcatgaacg ccagcttgat cttcttatta caactgaagc ccccaaaatg
    37741 gacgaatttt gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct
    37801 tctaaactaa agggagatct taactatctg cgactagagt gggggccaga ctttcaacag
    37861 catgaggcag gtttgatcgg tgctgacgaa gttcccattc tgacaaccag ttctgctgaa
    37921 ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt cagttgggca
    37981 cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat
    38041 gccatatggc tacaaaatag cgataaaaat gcgttgattc gcgatctatt gaaaattaac
    38101 gtgctggatg aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact
    38161 tcggagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
    38221 aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg
    38281 aactacgccc ctaattag
//
LOCUS       DAPQZD010000028        38145 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPQZD010000028 DAPQZD010000000
VERSION     DAPQZD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.028"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(24..305)
                     /locus_tag="RO454_001404"
     CDS             complement(24..305)
                     /locus_tag="RO454_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933256.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQQLSRSLIHENQA"
     gene            406..825
                     /locus_tag="RO454_001405"
     CDS             406..825
                     /locus_tag="RO454_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933257.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADKLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1091..1246
                     /locus_tag="RO454_001406"
     CDS             1091..1246
                     /locus_tag="RO454_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDY0933258.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            1406..1624
                     /locus_tag="RO454_001407"
     CDS             1406..1624
                     /locus_tag="RO454_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933259.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYQR"
     gene            2192..2380
                     /gene="dicB"
                     /locus_tag="RO454_001408"
                     /gene_synonym="ftsT"
     CDS             2192..2380
                     /gene="dicB"
                     /locus_tag="RO454_001408"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDY0933260.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKV
                     CIVSMLARLRLMPKGCAQ"
     gene            2377..2568
                     /locus_tag="RO454_001409"
     CDS             2377..2568
                     /locus_tag="RO454_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDY0933261.1"
                     /translation="MNPVFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            2661..5132
                     /locus_tag="RO454_001410"
     CDS             2661..5132
                     /locus_tag="RO454_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDY0933262.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGLQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITPTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADF"
     gene            5191..5394
                     /locus_tag="RO454_001411"
     CDS             5191..5394
                     /locus_tag="RO454_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDY0933263.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLIENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            5394..6419
                     /locus_tag="RO454_001412"
     CDS             5394..6419
                     /locus_tag="RO454_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDY0933264.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVDARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(6472..6561)
                     /locus_tag="RO454_001413"
     tRNA            complement(6472..6561)
                     /locus_tag="RO454_001413"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6525..6527),aa:Ser,seq:cga)
     gene            6655..7452
                     /gene="mtfA"
                     /locus_tag="RO454_001414"
     CDS             6655..7452
                     /gene="mtfA"
                     /locus_tag="RO454_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDY0933265.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7553..7628
                     /locus_tag="RO454_001415"
     tRNA            7553..7628
                     /locus_tag="RO454_001415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7586..7588,aa:Asn,seq:gtt)
     gene            7790..9052
                     /locus_tag="RO454_001416"
     CDS             7790..9052
                     /locus_tag="RO454_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDY0933266.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(9246..10550)
                     /gene="ybtS"
                     /locus_tag="RO454_001417"
     CDS             complement(9246..10550)
                     /gene="ybtS"
                     /locus_tag="RO454_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDY0933267.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(10578..11858)
                     /gene="ybtX"
                     /locus_tag="RO454_001418"
     CDS             complement(10578..11858)
                     /gene="ybtX"
                     /locus_tag="RO454_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDY0933268.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(11851..13653)
                     /gene="ybtQ"
                     /locus_tag="RO454_001419"
     CDS             complement(11851..13653)
                     /gene="ybtQ"
                     /locus_tag="RO454_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDY0933269.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWGHE"
     gene            complement(13640..15352)
                     /gene="ybtP"
                     /locus_tag="RO454_001420"
     CDS             complement(13640..15352)
                     /gene="ybtP"
                     /locus_tag="RO454_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDY0933270.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVVRLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            15609..16568
                     /gene="ybtA"
                     /locus_tag="RO454_001421"
     CDS             15609..16568
                     /gene="ybtA"
                     /locus_tag="RO454_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDY0933271.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFTPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            16759..22866
                     /gene="irp2"
                     /locus_tag="RO454_001422"
     CDS             16759..22866
                     /gene="irp2"
                     /locus_tag="RO454_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDY0933272.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            22954..32445
                     /gene="irp1"
                     /locus_tag="RO454_001423"
     CDS             22954..32445
                     /gene="irp1"
                     /locus_tag="RO454_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDY0933273.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            32442..33542
                     /gene="ybtU"
                     /locus_tag="RO454_001424"
     CDS             32442..33542
                     /gene="ybtU"
                     /locus_tag="RO454_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDY0933274.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            33539..34342
                     /gene="ybtT"
                     /locus_tag="RO454_001425"
     CDS             33539..34342
                     /gene="ybtT"
                     /locus_tag="RO454_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDY0933275.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRNITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            34346..35923
                     /gene="ybtE"
                     /locus_tag="RO454_001426"
     CDS             34346..35923
                     /gene="ybtE"
                     /locus_tag="RO454_001426"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDY0933276.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENLAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            36054..38075
                     /gene="fyuA"
                     /locus_tag="RO454_001427"
     CDS             36054..38075
                     /gene="fyuA"
                     /locus_tag="RO454_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDY0933277.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         8771 a        10991 c        10336 g         8047 t
ORIGIN      
        1 cattcatcgc catgcggatg tgttcatgct tgattttcat gaatcaagct cctagaaagt
       61 tgttgtgtta acgttttggt atcttccagc tcgggccaaa tattcatcca atcaaaaggc
      121 cttagttgct gacgtgtaac ttcaccatta ctggctcgct caataaggac acataacgat
      181 gcccctaaca cttgaccttt actcaatgcc tttcttagat aaccgatgct ggtaccacac
      241 tcgcatgcaa acatacgctg ttcatctgac gaaagagaat tgagaaatat tcttaattct
      301 tccatagcta ctccttagta aacacagcaa agaataccca caggtaaaca aaagtcaata
      361 cccacaggtt gtttaccttg cggtaatcgc atctattatt tacctatgga caaatatgaa
      421 tttagacgac agcaactcat caaaattcgt gatgagaaat gcgatggtaa agcggttaac
      481 gtggccagaa agatcgggcg cgagccttct tatgtatcaa gaatgttgta cccagagggg
      541 aaaaagggaa aaaaacggat cgctgatgat atggtggaga ttatcgaaga gtcctttggg
      601 ttaccccggg gatggatgga tggtatcgtt tcatcatcaa cgaacacagc ctccagttat
      661 gaaacaaggg ttctaacgcc acgacaacgt atttttttag atctcttaga cgaactgcca
      721 gaaagtgaag cggataaatt attaaaaact cttgaagaga aaaaacagta ttacaatatg
      781 atctacgaag aaatccgtaa aaagaaagca caaaacgcat catagctcac caaacaacta
      841 gtcaccagtt aagacaccgc aaaaatttac ccatgggtat ttacttttta aatacctatg
      901 ggtatccttc ttttcatacc aacccacccc gccccacaga atgcagggca atacttcgag
      961 ttaccaggca gtggtcaggg gttaagtagc cagcccgagg cgtaagaaca tgacggcagg
     1021 gttcaacttt aataactatg cagcaggttt ttgttccgct accccggcgt taaggggaaa
     1081 tgaggtcaac atggatacta tcgatcttgg caacaacgaa tctctggtgt acggcgtgtt
     1141 tcccaaccag gacggcacat tcaccgcgat gacgtatacc aaaagcaaaa cgtttaaaac
     1201 cgaaaatggt gcccgtcgct ggctggaaag aaactcaggt gagtgatatg gatttcgaca
     1261 caatcatgaa aaaggcttac gaagaatact tcgaaggcct taccgaaggc gaagaagctc
     1321 tcagcttcag tgagtttaaa caggcgcttt ccagctcggc aaaatctaac ggctgataag
     1381 cgaagcagca ccgcgaggaa tcagtatgca gaaacgagaa cccgtcatca tcgcgccaga
     1441 ctataccgat gatgaacttt atgagtggat gcgccagaaa attaatgcag cgcaggatct
     1501 gaaatgggcc aatgaagcca gggctaagca ggctgaaaat ctgtccgctc tggagcagga
     1561 tatcaccagg ctggaaaaag cagcggcatt aagcattgcc agaatgatta cataccagcg
     1621 ttaatagcta accaacgaag ctaaggttgg taattaagga gttctccacg ggtgaggtgg
     1681 agtgcgtgcg ccggacacgg gtgagcatcc ggcactgaca gtttactgaa aggatatttc
     1741 catgaaaagt cagaccataa cgcgaaagcg cacggcgagg tagctggttc atagatagcc
     1801 tgtcgttaaa ttttcgtcga ccgtgcgctt ccggttgtgg cactacgcga aatggcgcgg
     1861 cggtaagtat ggcggggtta ttccttcccc gttgaggaca ccgggttgtc aggttgacca
     1921 tacgcttaag tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg
     1981 gcggtatcag ttttactccg tgactgctct gcctcccttt ttaaagtgaa ttttgtgatg
     2041 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga
     2101 ttctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcaccg
     2161 catcacaaaa ctcattgttg agggcgcgat aatgaaaacg ttattaccaa acgttaatac
     2221 gtctgaaggt tgttttgaaa ttggtgtcac tatcagtaat cctgtattta ctgaagatgc
     2281 cattaacaaa agaaaacacg aacgggagtt attaaataaa gtatgcattg tttcaatgct
     2341 ggcccgttta cgtctgatgc caaaaggatg tgcacaatga atccagtatt tgcacttatt
     2401 ctgacggttt ttcttgtttc cggagagcca gttgatattg cagtcagtgt tcacagaaca
     2461 atgcaggaat gtatggcagc agcaaccgaa cagaaaattc ccggtaactg ttacccggtc
     2521 gataaagtta ttcaccagga taatatcgaa atcccggcag gtctttaaaa cagttccgta
     2581 ataaatatcc ggtttcattc ttatatgcca gcaatggcag ggatttgttc atccttaaat
     2641 ctgtcatgag gttaaaacaa atgagtaaag tctttatttg cgccgctatt cctgacgaac
     2701 tggcaacaag ggaagaaggc gctgtggctg tagccacagc cattgaagct ggcgacgaac
     2761 gccgtgctcg agcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact
     2821 gcgcttataa atttattgtc tgcgaggata aacctggcat accccgccct gccctcgatt
     2881 catgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcttttgtcc
     2941 cggttgagac tgaatccgat ccgatgaacg tcacttttga caagctggcc cctgaagtac
     3001 agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat atggttatta
     3061 gcgcacagga attgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt
     3121 tgatgaaaat gccagaagtt aacgccatgt atccggagct taagctgcac gccattgggt
     3181 gggttaagca taaatgtatt cctggtgcta aatggcccga aattcaggca gagatgcgca
     3241 tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg
     3301 atctcgcccg cgccagagcc aatcaacagt acactgaaaa ttcaacagga aaaatcagcc
     3361 cggtcattgc tgccattcat cgcgaataca agcagacatg gaaaacactg gatgacgaac
     3421 tggcctacgc tctctggcct ggtgatgtgg atgccggaaa cattgacggc agcatccatc
     3481 gctgggcaaa aaaagaagtt atcgacaacg accgcgaaga ctggaagcgt atctcggcat
     3541 caatgcgcaa acagcctgat gcccttcgct acgaccgcca aactattttt ggccttgtcc
     3601 gtgagcgtcc gatcgacatt cacaaagatc ccgtagcact gaacaaatat atctgcgaat
     3661 acctgacgac aaagggcgtg tttgagaatg aagaaacaga cctgggcact gttgatgttc
     3721 tccagtcatc agaaacacaa actgatgcag tggaaactga ggtatctgat atcccaaaaa
     3781 atgaaaccgc gccggaagct gaaccatctg tagagcgtga ggggccgttc tatttcctct
     3841 tcgcagataa ggacggagaa aaatacggtc gcgcaaacaa actctctggt ctggataagg
     3901 cactggctgc tggcgccact gaaatcacaa aagaagaata ttttgcccga aaaaatggca
     3961 catacacggg cttaccgcaa aatgtagata ccgctgaaga ttcagaacaa ccagagccga
     4021 taaaagttac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     4081 acgccgataa gttgcttgct gcatcacgtg gtgaatttgt tgaagggatt agtgacccga
     4141 atgatccgaa atgggttaag gggctccaga cccgcgattc tgtgaaccag aaccagcatg
     4201 aatcggaacg gaactaccaa aaagcggaac aaaacagtcc aaatgcgtta caaaacgagc
     4261 cagaaacgaa acaatccgaa ccagtagcgc aacaggaacc ggaaaaagtc tgcaccgcct
     4321 gcggtcagac cggcggcggc aactgccctg attgtggcgc ggtgatgggc gacgcaacat
     4381 accaggaaac attcgatgaa gagtatcagg ttgaagttca ggaagatgat ccggaggaaa
     4441 tggaaggcgc tgaacatcca cacaaggaga acactggcgg caatcagcat cacaatagcg
     4501 ataatgaaac tggcgagacg gcagatcacc caattaaggt gaacggtcat cacgaaatca
     4561 cacccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc
     4621 ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg
     4681 gaccggaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca
     4741 ccattaaatg gtggcttaag caatcacgcg aagcgcaatc tgccattatg accgatgaaa
     4801 tcccgttaga tgatgcactg ttacaattgc gagaatttat cgacgaaaac tccggtgaat
     4861 tttttgttca ggtctgggga aatggagcca acttcgacaa cacgattttg cgccgttcat
     4921 acgaacggca ggggatcccc tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa
     4981 tcgttgagct ggggaaagcc atagacttcg atgccagaac ggctattcca ttcgaaggtg
     5041 agcgccataa tgcacttgat gacgctcgtt accaggcaaa atacgtttca gctatctggc
     5101 aaaaactgat cccgagtcag gctgattttt aatgttcaac cctaattgcc gctaaccgta
     5161 tatagttagc ggcggttatg agatatagct atgagcagct tatttttaac cgaagatgaa
     5221 ttgctaatat taacgggctg caaatatgca agccaccagc gaaaatggtt aatagaaaac
     5281 gggcttccgt tctataccaa tcgtagtggc aaaccgattg tcagccggga tctatttacc
     5341 tgcaataaaa ctttaccacc acgcgaggta gagccgaatt ttggtgcgat ctgatgggaa
     5401 gacgaaggaa aaatcctgaa cacgaaaaat tacctccaaa tgtataccca aataaatata
     5461 gttatgtatg gaaaccaaca tccagagaat ctgtcacact aaccgccatc aaggatggtt
     5521 tagctgcttt atggaaaaag tatgaggaaa ctgtaaataa tcgcgatcgt gcaatgacat
     5581 tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta cagtgacctt agtccaagaa
     5641 cacaaaaaga ttatctgcaa catcaaaaaa agttgcttgc cgtattcggt aaggtaccag
     5701 cggattccat aaaaccagaa cacatccgtc gatacatgga caaaagaggg gagcagagta
     5761 aaacgcaagc caaccatgaa aaaagcagta tgtcccgtgt ttacagttgg gggtatgagc
     5821 gagggtacgt gaaggctaac ccatgtgcag gtgtaagtaa attcaaggcc aaaaaccgcg
     5881 aacgatatgt aaccgacaaa gaataccagg cagtattaag cgttgcacct cttcctgttt
     5941 ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt ttccgatgtg ttatcgctga
     6001 aatgggaaca gattggaaac gacgggatat tcatccagca agggaaaacc ggaaaaaaac
     6061 agataaaagc atggagtcca cgattacagg cagcgatcga aaaagcaaaa cagttaccaa
     6121 aatctgccta tgtgatcagc aatcaatacg gcaaccgata tatgtacaaa ggctttaacg
     6181 aaatgtgggt agatgcaaga aatcgtgctg gaaaaatttc aggtatttta accgacttca
     6241 cctttcatga tctgaaggcg aaaggaattt cagactatga aggaagcagc cgggataagc
     6301 aacttttctc tggtcacaaa accgaagggc aagtgctaat ctatgacagg aaggttaaag
     6361 tttcaccaac acttgatgtc ccgttacctg aaaatattcc aagaaaatat tccaagtaat
     6421 tccaagtgtg atttttgtca ctgacttaat gatgtgtaag tgattgaatt ttggcggaga
     6481 gagggggatt tgaacccccg gtggagttgc ccccactccg gttttcgaga ccggtccgtt
     6541 cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa tttggcattt
     6601 taacagtccc tgtccgtgca attttgttca agtgacgagt ttgcgagcaa aacgatgatt
     6661 aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg gcaggaagca
     6721 ctatcgatcc cccttttaac gtgtctgaca gaacaggaac aaagcaaatt ggtcgctctt
     6781 gccgaacgtt ttttacagca aaaacggctt gttcctttac agggctttga gctgaattca
     6841 ttaagaagct gccggatagc acttctattt tgcctgcccg ttctggagtt aggactggaa
     6901 tggctggatg gttttcatga aatcttaatt tatcctgcgc catttgtggt cgatgatgaa
     6961 tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg tcagagctgg
     7021 cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga tgcttctggt
     7081 tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc
     7141 agcggagttc cctttatttc gttgcgtgag gttgctggct gggaacacga tcttcatgct
     7201 gcaatgaaca acattcagga agaaatcgaa ttagttggtg agaatgcggc gagcattgat
     7261 gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac tttctgaata tttctttagc
     7321 gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg tcaattttat
     7381 caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt ttcggcgacg
     7441 aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatggc ttatgaaatt
     7501 tagtgttgac agacaaggta ccgctaagta atatgcgccc cgttctcacg attcctctgt
     7561 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     7621 aggagccaat tttctgtttt catgcatcct tgcgaatctt tatctgatgt tgattcaaca
     7681 ggttagtatg aaaactcttc tcagtgcatt ttgattttac cctacgcatc gggaaaaatt
     7741 ggtggtcaaa tctggggtca ggttagttcg ataatggagt gacccccata tgtcccttac
     7801 cagcgcaaaa atccgcaccc tcaagccttc tgataaaccc tttaaagtct ccgattctca
     7861 cggtctgtat ctgcgggtca agccgggtgg ctcccgccac tggtatctca aataccgtat
     7921 tagcggtaaa gaatcccgca ttgcgctggg tacctatcca gccatctccc tgtctgatgc
     7981 gcgacagcaa cgtgaaggta tccgtaaaat gctggcgctg aatatcaacc cggtacagca
     8041 gcgggctgct gtacgtggct cacgaacacc ggagaaagtt tttaaaaacg tggcgctggc
     8101 gtggcataaa agtaacagga aatggtcgca gaacaccgcc gaccgtctgc ttgccagcct
     8161 gaacaatcac atctttccgg tcatcgggaa cctacctgta tcagaactta aaccccgtca
     8221 tttcattgac ctgctgaaag ggatcgagga aaaaggtctg ctggaggttg cgtcccgcac
     8281 acggcagcac ctgagtaaca taatgcgcca tgcggtccat caggagttaa tcgatacgaa
     8341 ccctgcagca aaccttggcg gcgtgaccac acctcctgtc agacggcact atcctgccct
     8401 gccgctggag cggctgcctg aactgcttga acgtattggg gcatatcatc agggccgtga
     8461 actgacccgg catgccgttc tgctgatgct gcatgtgttc attcgctcca gtgaactgcg
     8521 tttcgcccgc tggtcagaga ttgatttcac aaaccgagtc tggacgatac ccgcgacgcg
     8581 agaacccatt attggcgtgc gttattccgg ccgcggggca aaaatgcgaa tgccgcatat
     8641 cgtccccctc tcagaacagt ccatcgccat tctgaaacag attaaggata tcaccggtaa
     8701 taatgaactg atcttccccg gcgaccataa cccgtataag ccaatgtgtg aaaacacggt
     8761 caataaggca ctgcgggtga tgggttacga cacgaaaaag gatatctgcg gtcacggctt
     8821 ccgggcaatg gcatgcagtg cgctgatgga atcgggttta tgggcaaagg acgcagtaga
     8881 acgccagatg agtcatcagg agcgcaatac cgtgcgcatg gcttatattc ataaggcaga
     8941 gcacctggaa gcccgcaaag cgatgatgca gtggtggtcg gattatctgg aagcatgccg
     9001 agaatcttat gcaccgcctt atacaattgg taaaaataag tttatcccat agtatactaa
     9061 cgaatcccac actctggcaa attaccacca cctccacaag aggtggttct atatagactt
     9121 ttatcaactt catgacgaaa aacacctatt ttatcccaaa agaagacaat agagtcacag
     9181 aggaatcaaa gagcgactca attgtctttg gacagtctgg ttgtgaggtt agaaaacagt
     9241 tactcctaca ccattaaata gggcgcaatg ctcgctaatt tctcccgggt ttctgtcagt
     9301 tcgcgttccg gcgtagattg cgcgatgatt cccgcccccg cctgtatcca gcagcgctgg
     9361 ctatcctgaa atacggaacg cagaactagc gccgcatcaa agcgcgtatc gtccagcagc
     9421 aggattgcgc cggaataaag ctctcgcggc gtcttctcaa tttgcataat cgcgttcaga
     9481 gcagcatttt tagggatacc tgaggcggta atcgacggaa acagcacggt aaacgcatcc
     9541 caggcatcct tgttttccgc aagctgaccg ctcacgccag accccagatg ctgaacgctg
     9601 ccgcgctggc gaaccgacat taaatcttca accaccacgc tgcccggctg gcaaacggcc
     9661 tccagttcag caatagcttc tttgacagaa aggatatgct caagcacctc tttactgtcg
     9721 tgcagcagtt ctgcctcttt cgccttatta tgctccgggt ttcccatgcg atcgcgggtg
     9781 cccgcaagcg gttcagtgac cactttattg cccgtcactg acatcaccag ttctgggcta
     9841 aagcccagcg cttcgcgtcc ttcctgacgg aacataaacg agcgcacagg cgtgtttgcc
     9901 tgccgcccgt ataacagcgt ggcgggcata tcaatccgcg atggcagggg aatggcgcgc
     9961 gagacaatca ctttgacata ctcgccacgg cggatctccg caacggcgcg cgcaacctgt
    10021 tgtttatatg cctcaccatt gagggcggta tccaccgcca gtggtgcgtt ctgcgtcgtt
    10081 gtaccggcct cttttaccca ctcgcacaaa cgtcggcacc cgtcggcgga gtccgcataa
    10141 acggtgacat ttcccttttc aaaaataagt tcttcacggg gaacggttaa cgtcagcagc
    10201 ggccactccc cggcgttaaa ggcaatcccc cgcgcatgcg ccgcaaagtt gaacccaacc
    10261 tgtccatata cccgacgtcc tttcacctga ggatgcgcca taaaacgacg cgcgcagtcg
    10321 gcaatggaat ccaccgccca tttttgctcc ccggcatcat caataaatgt cgcctgcgtt
    10381 ccgtcggcat taatgtgcag ccgtgcctgg caccctttgc ctaaatacca acagggttga
    10441 cgctcataga cataacactc ttcttctgcg aactggcgta aaacgccaga aatcgtcggt
    10501 agccattgtt cctctggtaa cgccagatgt agaaattcac tgattttcat cccttacctc
    10561 tctgtgttat tcgcggatca atcaattgcg cttgtcatca gttctcttgc cgaatgcagc
    10621 cggatataag caacccacgc cgccagcaac gtgaatgccc cggcaaacag gaagcaggcc
    10681 gcatagccaa aatgttgagc aacaacgcca ccgataacgc ctgccagaat ggcgattgcc
    10741 gcatcagtac attgaaagag ggtgaagtcg acaccggcct gcaaaggcga tgaaagcgac
    10801 atcagcgtgg cgtaaagaca gaccagcgcg caggccagcg aaatggactg gacaatgacc
    10861 agacactgga gaatctgcag cagcagatga cccggcgcca tcatgagcgt catcaccacc
    10921 gccagcagcg cgatcccctg gacgccataa gccgtcaaca gcgctctgcc gggtgaggtg
    10981 tatttcatca gcaatccgcc ggccagcgtt cctgctatgc ccgctgcaat attgccgccg
    11041 ctgaacagcg cgcccaatgc ggacatgctc aacccatgat ccaacaacag cggtgccaga
    11101 agaggcagca caaaccgcat gcctgaattc agcatcaata ccagcagtaa gcccaggcgc
    11161 gcctgcttcc tccttagcgc ataacctaac gccggaacat gcgggatagt cgctgttcgc
    11221 gtcggctccg taatgcgcca cagcgggagt gacagcgcca taatcagcac cgccatcatc
    11281 agcatggcga caggccagcc ggatgccgcc gacagcatca tgaacacccc gccgccgcac
    11341 atcattccca gatagctgcc gccgacctgc acgctgtttc cccagccgta acccgtgcgg
    11401 gtcagttgat cgacacaaaa gccgtcgctg gcgatatcaa tggtggaggc gaccgtgcct
    11461 gccaccataa acagaacaaa taacgcgacg atttgcgtat cggggaatcc cccttctcgc
    11521 ccaaaccagc caatcgctgc ggcaatagtc aggatcgccg ctagcgcgac ctgaccacgc
    11581 aggattaaca tgcgggaacg gcgttcctgg ctaccgggcg gaagccgcca gcgctcgatc
    11641 cacggcgccc aaataaactt cagcgcccag ggcagcataa acagggttgt cgcaccggca
    11701 agcgccagcg aaccgccagc agcgcgcacc agcgcgggta aggactgcat agacagcgcc
    11761 gataccagac tctgcgtcac atagacgccg gcaatagcaa aaatcacccg cccggtcgtc
    11821 aacgtcagcg gtttcacatt cgactgaaca tcactcatgc ccccactctc cagacgcgga
    11881 aaccccgtcg tcgcgccaca cgcgcgcggc catttgcgcc tgccacagcg cctgataacg
    11941 tccatgatgt gagagcaatt gcgcatgagt accctgctca accacctgtc cctcttccat
    12001 caccagaatg tttccggccc cggcgatggt ggataaacgg tgagcgatga taatcaccgt
    12061 ccggttgtaa accaggttat cgatcgcttt ttgcaccgcc agctcgcttt caatatccag
    12121 cgcggcagtc ggttcatcga gaataacgac cggcgcgttt ttcaataacg ctctggcaat
    12181 ggaaatccgc tggcgctcgc cgcccgatag ctggccgccc atctctccca ttggcgtcag
    12241 ccagccttgc ggaagacggg aaataaactc aaggcactgc gccgcgcggg cggcctcttc
    12301 tacctcctgc cgcgtcgcct gtgggcgcgc gatacggata ttcgccagca gcgtgtcatc
    12361 aaacagccag acatcctgga aaacaacaga aatcaggcta ttgagctgtt ccggcgtcag
    12421 gcggcgaata tcgacgccgc caatagaaat ctgcccttgc tgcggatcgg catagcgcat
    12481 taacagtttg gtgacggtag ttttgcctgc gccggaggca cccaccagcg cgctcatact
    12541 ggctgccggg aacgtcaaag aaacatgatt aagcgcgtgg ccgtcacctt cttcgtagcg
    12601 atagctgacg ttgtcaaagc ggatatcgta acgttccggc atctcgcttt gctctgcgac
    12661 gggaagcggt gctatcgcca taaactgctc aatcctttgc agggcgctgg cgatcagttc
    12721 cacaaccgag gtgtagctga taaacatcgc catcggttct gcgaagcgca taatcatcgc
    12781 gacggcggca atcaaaaagg cgaggttcag agtgcccgtc actacccaga cgatcccgga
    12841 taacaccacc acctgtaggc ccaactccac gacgctggcg atcagcatcg tcgcgccagc
    12901 gccctgacgg tgagtgcggg tctgtaagtt ttccagcgca ttgaaatgcg ccagcagcgc
    12961 ccggctttta tcggcatcgc tgccgcaggt gcgcaatacc atcatcccct gagcaaattc
    13021 aacgatatcg ccgctcagac gctggtgcgc ttcccccagc gtctgcattt gtcgccgcat
    13081 cgccgggcgg cgccagtaat aaaacggcac cagcagaggg aagatcagca acatcaccag
    13141 ccccagccgc cagtctatcc acaatgtcgc cagcgacgcc gtcagcggcg tgacaatggt
    13201 gagcagcaaa atattggcta tcgcaataac ataattgagg ttttcatcca cgctgcccag
    13261 cagcaaggcg ttcatttcac ccgccctgcc gcgctggagc ttctccagcg gcacgcggcg
    13321 taactgctcg ccaagtcgca ggcgcaactc atgggtagcc tgcgccagat gaccacggta
    13381 ttcaaagccc aggccatacc agcgtagcac cagcgtcaca attgcggcga cgctgaaggc
    13441 catagcccag ttaagaagtt gcggcgcgtc tccccgtaac agcgcatcaa tgatcggata
    13501 aagacaggca aaggcgatcc cctgcatgaa tgccgccagc aacagcgcca gcatactgcg
    13561 tcgcagcatc ctggcctgac tgccaacgct ggagataagc tggcgccaga tgacgcgcca
    13621 ggccaggtta tccgcaggat tattgtcttt cattttccat cacctcttcc tgtgttccac
    13681 ccggcaccca atgctgcgcc tgctgatagt gttgccagag ccgttgatac aggccgccct
    13741 gcgccaacag ttgcgtatgg ttccccattt ccctgagctg tccgtcggaa aacagcaata
    13801 tcacatcggc ctgagtcacc attgagagac gatgcgcgac catgatgacc gtccggccac
    13861 gcatggccgc cgcgagcgcc ttgataagcg ccgcttcgtt ttccgggtca gcaaacgccg
    13921 tcgcctcatc gagcaccagg atggggcgat cctgcaaaag cgcccgggcg atagtaatgc
    13981 gctgccgctg gccgccggag agaaataccc cacgttcccc gactcgagtg ttgtaacctt
    14041 ctggcagagc gctaataaaa tcatgggcct gcgccactct ggccgccgct ataaccgcct
    14101 ccagcggcgt atccggcgcg cccagacgaa tgttattggc tatcgtgtcg gcaaaaagga
    14161 agttgtcctg aaacacaaac gagagttgct tcatcagggt gtccgtctgc atatcacgca
    14221 gatccacgcc gccaatacgg atatggcctt tgtccgggtc ggcgtaacgc agcagcaagc
    14281 gcaccacggt gctttttccg gcgccgcttg gcccgaccag cgccacaatt tgcccggcag
    14341 gcacatgaaa gctcacctcc tgcaacgcgg cgccagtacg cgcttgcgga taatgaaagc
    14401 tcacctgctc aaaggtaatg ctcgcctctt gcggctgctg gtcagactgc ggcagcgata
    14461 actccggcat cgcgagaacc tgataaatac gctgaatgct taaacgcgtt tgcgcgacca
    14521 ggttattgag catcatcatt ggcattacgg cttcggccat tccgctgccc agcagtaaaa
    14581 cggccaccca cgcgataaaa tcgaaactgc catagtgcaa caggccgtat cccgaccaga
    14641 tcaggacaaa cagggtcggg agaggattca gaatcgagaa ggaaaaacgc gctgaaaaac
    14701 cggctttgcg ataccaggtt ttgagcacat cgacccactc ttcaagggcg cgttgatagc
    14761 gcaaaaaact ggtgctgccg ctatcaaacg ttctcaccac gggcatggcc tgcacgaact
    14821 caataaccgc cgcgctaacc tgctcgcgcg ccttatgata acgctgagcc atatcttccg
    14881 aacgctggcg ggcgagcacg agaacaaccg atccaaacgc cagtaccccc agcgtcgcga
    14941 ttgccagccg ccagtccagc cagaacaaga taacgattgt cgccagcggc atgatgatcg
    15001 cgcgggcata gagcggcgtg ctgtcggcca caaaaatatg taacgacttc acgtcatcca
    15061 gcatcacttt cgccactgac ccgctgccca tttgctgtaa cacgccaggc ggaagctgca
    15121 atgctttacg ggctaacctg ctgcgcagaa tgttctccag atgaaaagcc gcgtaatgag
    15181 aggtgttgaa cgcctgtaag cgcagaacaa aagttaagac cacgcagccg atcacgccgc
    15241 ccagcggcca ggccggaata gcgtcaggcg tcgcgcgaat gtcacgcagg ctccacgcca
    15301 gaaaaaggag cgcccccagg ctggttaatg ccgccagccc cgccagcgcc atagctaaaa
    15361 taaccttcct gcgaaccggt gtaatcacct gccaaagagg aaatcgggac agcgattcag
    15421 tattggatga ttgagatgac atgggagtaa ctgaatttcc tgatgaattt ttacggacgc
    15481 caaaaacgac aattcagggt ggcgttctga gaattaatgt gaataataac cattatcaat
    15541 aactcatcta ccccattcgg ttctatttat acccgcattg gtctaagcca cagggagata
    15601 accaggtcat gacggagtca ccgcaaacgc aatctgaaat ctctattcac cagttggtgg
    15661 tcgggaaacc tgcaaacgat ggcaatattc ctgctcagtg tgaactgctg cgctgttcgt
    15721 tgcaggaagg tatggatatt ttgctctgga ggggccattt tgcgcggcct gagacgttgc
    15781 agctacacga cgatctgggc aggattaact tctcgtgcat cctggaaggc acatcgcgct
    15841 ttgcgattca gggactccgg cgtcacaccg actgggagct tgcgcgcaat cgccattaca
    15901 ttacccatac gccagactgt cgcggcagcg cctcctattg tggccgattc gagagcatta
    15961 ccctctcctt cagccctgaa accctcgcgc tgtgggtgcc ggatattagc gcggtcatta
    16021 agaataagat cgactcccac tgctgttgcc agcagcaccg ttgtaatgct gaaacccact
    16081 taacggctca ggccttgcgt cacgcgctga tgcgcatgca cagcggcttc agtcatgaac
    16141 aaaagccgtc gacgctatgg ttgcaggggc aaagtctggt catgctcagt ctggtgctgg
    16201 atgaacatcg cgaagacgcc agttgcctct cctgccactt caccccgatg gaacgccaga
    16261 aactgctgcg ggcgaaggat ctgctgctgg ctgatctgac gcaagcgccg ggcgtcgcgg
    16321 agctggccag agagtccggt ctgagcgttc tcaaaattaa gcgaggcttc cgcgttctgt
    16381 tcaacaacag cgtctatggc ctgtttcagg ctgaacgaat gcaggaagcc agaagacgcc
    16441 tggccaacgg caatacctcc gtcatgacgg tggcggccga tctgggctat gccaacgcca
    16501 gccatttctc tgccgccttc cagaaacagt ttggcgtaac tccttcgacc tttaaacgcg
    16561 ggatgtaacc ggggtcgcgc ccccctaaac tcctccctga cagaggcccg gataccccat
    16621 cagggcctct gttctttccg cctgttacca acccgaaacg ggcataaata aacccgtttc
    16681 gggtagcatc caattgttaa taattattat tctcatatga gtaatgcttt tcggtaagac
    16741 gtgccatcag gaggaagaat gatttctggc gcaccatctc aggattcgct gttaccggac
    16801 aaccgccacg cggctgatta ccaacaatta cgcgagcggc tcatacagga actgaattta
    16861 acgccgcagc agttacatga agagagcaac ctgatccagg ccggcctgga ttccataaga
    16921 ttgatgagat ggttacactg gtttcgtaaa aatggctacc gccttaccct tcgcgagctg
    16981 tatgccgccc ccacgctggc ggcatggaac cagttaatgc tcagccggtc gccggagaac
    17041 gcggaagaag aaacgccgcc cgacgaatca tcctggccga acatgaccga aagtaccccc
    17101 ttcccattga cgccagtaca gcacgcctac ctgacgggcc gcatgccggg gcagacgctt
    17161 ggcggcgtgg gttgccacct gtatcaggag tttgaaggcc attgtctgac ggcgtcgcag
    17221 ctggagcagg ccatcacgac cttgctgcaa cgccacccaa tgctgcatat cgcctttcgc
    17281 cccgacgggc agcaggtctg gctaccgcaa ccttactgga acggcgtcac cgttcatgat
    17341 ttacgccata acgacgctga aagccgccag gcctatctgg acgcactgcg ccagcgcctg
    17401 agccaccgtc ttttacgcgt ggaaatcggc gaaacgtttg attttcagct gacgctcttg
    17461 ccggacaatc gccaccgcct ccatgtcaat attgacctgc tgattatgga tgcctccagc
    17521 tttacgcttt tcttcgatga gcttaacgcc ctgctggccg gagaatcgct gccggctatc
    17581 gacacccgct atgatttccg ctcgtatttg ctgcaccagc agaagatcaa tcaaccactg
    17641 agagacgacg cgcgcgctta ctggctggcg aaagcatcga cgcttccccc cgcgcccgtc
    17701 ttgccgctgg cctgcgaacc cgccacgcta cgtgaagtcc gtaatacccg acgccgcatg
    17761 attgtcccgg caacacgctg gcacgccttt agcaaccggg ccggcgagta tggcgtgacg
    17821 ccgacaatgg cactggcgac ctgtttttct gccgtgctgg ctcgctgggg cggcctgacg
    17881 cgtctgctgc ttaacatcac cttattcgac cgccagccgc tgcacccggc ggttggcgcg
    17941 atgcttgccg acttcaccaa tattcttctg ctggataccg cctgcgatgg cgataccgtc
    18001 agcaacctgg cgcgtaaaaa ccagctcacg tttacggagg actgggagca tcgccactgg
    18061 tccggcgtcg aattactccg tgaactcaaa cgccagcagc gctaccccca cggcgccccg
    18121 gtggtattta ccagcaatct ggggcgttcc ctctacagca gccgcgcaga atcgccgttg
    18181 ggcgagccgg aatggggcat ctcgcaaacg ccgcaggtct ggatagatca tctggcgttc
    18241 gagcatcacg gcgaggtctg gctacaatgg gacagcaacg acgcgctgtt ccctccggcg
    18301 ttagtcgaaa cattgttcga tacctactgc cagttgatta accaactctg cgatgacgaa
    18361 agcgcctggc aaaagccgtt cgcagatatg atgcccgcca gccagcgcgc gatacgcgaa
    18421 cgggtcaacg ccaccggcgc ccccattccc gaaggcttgc tgcatgaagg cattttccgt
    18481 atcgctctgc aacagccgca ggcgctggcg gtaacggaca tgcgttatca gtggaattat
    18541 catgagctga cagactatgc ccgccgttgc gcgggcaggt taatcgagtg cggggttcag
    18601 cccggcgata atgtggctat cacgatgtcg aaaggtgcag gacaacttgt tgcggttctg
    18661 gccgtcctgc tggctggggc ggtttacgtt ccggtttcgc tggatcagcc tgccgcacgg
    18721 cgcgagaaaa tctacgctga cgccagcgtc cggctggtgc tcatttgtca gcacgacgcc
    18781 agcgccgggt cagacgatat tcccgtcctt gcctggcagc aggccattga ggcggagccg
    18841 atcgccaacc cggtagtacg cgcccccacg caaccggcct acattatcta cacctccggc
    18901 tctaccggta cgccgaaagg ggtagtcatt tctcaccggg gagcgcttaa cacctgttgc
    18961 gatatcaata cccgctatca ggttggcccg catgacaggg tgctggccct ctccgcccta
    19021 cattttgatt tatcggttta cgacattttt ggcgtactgc gcgcgggcgg cgcgctggtg
    19081 atggtgatgg aaaatcaacg gcgcgatcct cacgcatggt gtgagctgat ccagcgccat
    19141 caggtcacgc tctggaacag cgtcccggcg ctgttcgata tgctgctgac ctggtgtgaa
    19201 ggtttcgccg acgccacgcc ggaaaacctg cgcgcagtga tgctttccgg cgactggatc
    19261 gggcttgacc tccccgcccg ttatcgggcc ttccggccac aaggacaatt tatcgcgatg
    19321 ggcggtgcca ccgaggcgtc tatctggtct aacgcctgcg aaattcacga cgtccccgcc
    19381 cactggcgct ccatccctta cggttttccg ctaaccaacc aacgctaccg ggtggtggat
    19441 gaacagggcc gggactgccc tgactgggtg ccgggtgaat tatggattgg cggcattggg
    19501 gtcgcggaag gctatttcaa cgatcccctg cgtagcgagc agcaattttt gacgctcccg
    19561 gacgagcgct ggtatcgcac cggcgatctc ggctgctact ggccagatgg cacaatcgag
    19621 ttcctcggtc gtcgcgacaa gcaggtcaaa gtcggaggat atcgcatcga gctgggcgaa
    19681 atcgaaagcg cgctcagcca gctggcgggg gtgaaacaag caaccgttct ggcgatcggc
    19741 gaaaaagaaa aaacgctggc ggcatacgtt gttcctcagg gcgaggcttt ttgcgttacc
    19801 gatcatcgga acccggcact gccgcaggcg tggcacacgc ttgcgggaac gttgccctgt
    19861 tgcgccatct cgccagagat ctccgcagaa caggtagccg atttccttca gcatcgcctg
    19921 ctaaaactga agccgggtca caccgctggc gccgatcctc tccccctgat gaactcactc
    19981 gctatccagc cgcgctggca ggccgtggtg gaacgctggt tagcatttct ggtgacacaa
    20041 cggcgactga agcccgctgc tgaaggttat caggtctgcg ctggtgaaga acgcgaggat
    20101 gagcacccgc acttcagcgg acatgattta acgttatcgc aaattcttcg cggtgcccgt
    20161 aacgaactgt cgttactgaa cgacgcgcag tggtcgccgg aaagcctggc ctttaaccat
    20221 ccggccagcg ccccgtatat tcaggaactg gcgacaattt gccaacagct tgcacagcgc
    20281 ttacagcgcc cggtgcgcct gcttgaggtg ggaacccgca ctggccgcgc cgcagaatcg
    20341 ctgttagcac agctcaacgc cggacagatt gagtatgtcg ggcttgagca gagccaggag
    20401 atgctgctga gcgcccggca gaggctcgcc ccctggcctg gcgcccgtct gtccctctgg
    20461 aatgcagaca cgctggcggc gcacgctcac tcggcggaca ttatctggct taataacgcc
    20521 ctgcatcgtc tgctgccgga agatcccggg ctccttgcga cattacaaca gcttgccgtt
    20581 cccggcgcgc tgctctacgt gatggagttt cgccagttaa cgccgtccgc cctactcagc
    20641 acgctcctgt taaccaatgg gcagccggag gccttgctgc ataacagcgc cgactgggcg
    20701 gcattattta gcgcggccgg cttcaactgt cagcatggcg atgaggtcgc ggggttacaa
    20761 cgcttcctcg tacaatgtcc tgacaggcag gtgcgccgcg atccccgtca acttcaggcc
    20821 gccctcgccg ggcgtctgcc ggggtggatg gtgccgcaac ggatcgtctt cctcgacgcc
    20881 ttaccgctga cggctaacgg gaaaattgac taccaggcgc tgaagcgtcg tcatacccct
    20941 gaagcggaaa acccggccga agcggattta ccccagggcg acattgaaaa acaggttgcc
    21001 gccctctggc agcaactctt atcaactggc aatgtcacca gagaaaccga cttcttccag
    21061 caaggcggcg atagcctgct ggcgacccgt ctgaccgggc aacttcatca ggcaggttat
    21121 gaagcgcaat taagcgacct gtttaatcat ccccggctgg cggattttgc cgccacgctg
    21181 cggaaaaccg acgtcccggt cgaacaacca ttcgtccact cccctgaaga tcgctaccag
    21241 ccctttgcgc ttaccgacgt gcagcaggct tacctagtgg ggcgtcagcc gggctttgcc
    21301 ctgggcggcg tcggctcaca tttctttgtt gaatttgaaa ttgccgatct ggatctcacc
    21361 cggttggaga cggtctggaa ccgattaatc gcccgccacg atatgctacg cgccgtcgtg
    21421 cgtgatggac agcaacaggt gctcgaacag acgcctcgct gggtaatacc cgcacacatc
    21481 ctccatacgc ctgaagaggc gttgcaggtg cgcgaaaaac tggcccatca ggtactcaac
    21541 cccgaagtct ggccggtatt cgatctccag gtcggttacg ttgacgggat gcccgcccgc
    21601 ctgtggctgt gtctggataa cctgttactt gacgggctga gcatgcagat tctgttggcg
    21661 gagctggagc acggctaccg ctacccgcaa cagctgcttc cgccgctgcc cgtcaccttc
    21721 agggattatc tgcaacaacc ctcgctacag tcgcccaatc cagattctct ggcatggtgg
    21781 caggcgcagc ttgatgatat tcctccggcg cctgcgttgc cgctgcgctg cttgcctcag
    21841 gaggttgaaa caccgcgctt cgcccgcctg aacggcgcac tggacagcac gcgctggcat
    21901 cggctgaaaa aacgggcggc tgacgcccat ctcaccccgt cggccgtact gttgtcggtg
    21961 tggtcaacgg ttctctctgc atggagtgca cagcctgagt tcacgcttaa ccttacgctt
    22021 ttcgacaggc gaccgctgca cccgcaaatc aaccagattc tgggcgattt cacctcgctg
    22081 atgctgctga gctggcatcc cggcgaaagc tggctgcaca gcgcgcagtc actacagcag
    22141 cggctgagcc agaacctcaa ccaccgcgat gtgtcagcca tccgcgtgat gcgtcaactg
    22201 gcgcaacggc aaaacgtgcc agccgttccg atgcccgtcg tctttaccag cgcgctgggc
    22261 tttgagcagg ataacttcct cgcccggcgt aatctgctca aaccggtctg gggcatctcc
    22321 cagacgccgc aggtctggct cgatcaccag atttatgaat ccgaaggcga actgcgcttt
    22381 aactgggatt ttgtcgccgc gctgtttcct gccgggcagg tggagcgcca gtttgaacag
    22441 tattgcgcat tgctaaaccg aatggccgag gatgaaagcg gctggcaact gccgctcgcc
    22501 gcgctggtgc ctcccgttaa acacgcaggg caatgcgcag agcgctcacc gcgcgtatgc
    22561 cctgagcact ctcagccaca cattgcggcg gacgagagca ccgtcagcct gatttgcgac
    22621 gccttccgcg aggtggttgg cgagtctgtc acgcccgcag aaaacttctt tgaggcgggc
    22681 gcaacgtcgc tgaatctggt gcaactgcac gttttgttac aacgtcacga attttccacc
    22741 ctgacgttgc ttgacctctt cacccaccct tctcctgctg ccctggccga ttatctggcc
    22801 ggcgtcgcca cggtggagaa aacaaaacga cctcgccctg ttcgccgtcg tcagcggcgg
    22861 atatagcgcg aagcaaactg attttccccg gaacgccatc gcgaacgcat ggcgttccat
    22921 tgactttgtg aatcttagga aacgggaccg attatggata acttgcgctt ctcttctgcg
    22981 ccgacagcag attccattga tgcatcgatc gctcaacact acccggactg cgaacctgtc
    23041 gcggttatcg gctacgcctg ccattttcct gaatcgccgg atggcgaaac gttctggcaa
    23101 aatctgctgg aaggtcgtga atgcagccga cgctttacgc gcgaagagct tctggccgtc
    23161 ggtctggatg ccgccatcat tgacgatcct cattatgtca atatcggtac ggtgttagac
    23221 aacgccgact gcttcgacgc caccctgttt ggctattcgc gacaggaagc ggagtcgatg
    23281 gacccgcagc agcgcctgtt tttgcaggcg gtctggcatg cgctggaaca tgccggttat
    23341 gcccccggcg ccgtccccca taagaccggc gttttcgcct cttcccggat gagtacctac
    23401 cccggtcgcg aagcattgaa cgtgacagaa gtcgcgcagg taaaaggtct gcaatctctg
    23461 atgggcaatg ataaagacta tattgccacc cgcgccgcgt acaaactcaa cctgcacggc
    23521 ccggcgttat cggtacagac cgcctgctcc agctcgctgg ttgccgtgca tctggcctgt
    23581 gaaagcctgc gcgcaggcga atccgatatg gcggttgccg gcggcgtggc gctctctttc
    23641 ccccagcagg caggctaccg ctaccagccc ggaatgattt tctctcctga tggtcactgt
    23701 cgtccctttg acgcctcggc tgagggcacc tgggccggta acggtctcgg ctgcgtggtg
    23761 ctgcgtcgcc tgagagacgc gctgctgtca ggcgatccga ttatctcggt gatcctctcc
    23821 agcgcggtca acaacgacgg caacagaaag gtcggctata ccgccccttc cgtcgcaggg
    23881 caacaagcag tcatcgaaga ggcgttaatg ctggcggcca tcgacgacag gcaggtaggt
    23941 tacattgaaa cccacggcac cggcacaccg ctgggcgacg cgattgaaat tgaagcgtta
    24001 cgcaacgtct atgcgcctcg cccgcaggat cagcgctgtg cgctcggttc cgtgaaaagt
    24061 aacatgggcc atctggatac cgcggcgggc attgccggac tgctgaaaac cgttctggca
    24121 gtcagtcgcg ggcaaattcc tcccttactg aattttcaca cccccaaccc ggcgctgaaa
    24181 cttgaagaga gcccctttac cataccggtg tcggcacagg catggcagga cgaaatgcgc
    24241 tatgcgggcg tctcctcctt tggtattggc ggcaccaact gccatatgat cgtcgcctcg
    24301 ctgcccgacg cgctcaacgc gcgcctcccc aatacggata gcggcagaaa aagtaccgcg
    24361 ctgctgctca gcgccgccag cgacagcgcg ttgcggcggc tggcgacgga ttatgccggg
    24421 gcgctgagag agaatgcgga tgccagctct ctggccttca cagccctgca cgcgcgccgt
    24481 ctcgatctcc ccttccgcct ggcggcgcca ttaaaccgtg aaaccgccga ggcgctcagc
    24541 gcctgggccg gtgagaaatc gggggcgctg gtttacagcg gccacggcgc cagcggcaag
    24601 caggtgtggc tgtttaccgg ccagggctcg cactggcgca ctatgggtca aacgatgtac
    24661 cagcactcaa cggcgtttgc cgacacgctg gatcgctgtt tttccgcctg tagcgaaatg
    24721 ctcacgccgt cactgcgcga agcgatgttt aaccccgatt cggcgcagct ggacaatatg
    24781 gcctgggcgc agccggcgat tgtcgcgttt gaaatcgcga tggcggcgca ctggcgtgct
    24841 gaaggactga agccagactt cgccattggg cattccgtcg gtgaatttgc cgctgccgtt
    24901 gtctgcggac actatacgat tgaacaggtc atgccactgg tttgtcggcg cggcgcgcta
    24961 atgcagcagt gcgcaagcgg cgcgatggtg gcggtatttg cagacgaaga cacgctgatg
    25021 ccgctggctc gccagtttga gctggatctc gccgccaaca acggtacgca acatacggta
    25081 ttttccgggc cggaagcccg tctcgcggta ttttgcgcca cgctctcgca gcatgacatt
    25141 aactatcgtc gcctgagcgt aaccggtgcg gcgcactccg ctttactgga gccgatactc
    25201 gatcggttcc aggacgcctg cgcgggactg cacgcggagc cggggcaaat accgattatt
    25261 tccacgctca ccgccgacgt cattgatgag tcaacgctca accaggcgga ttactggcgc
    25321 cgacacatgc gccagccggt gcgttttatc cagagtattc aggtggcgca tcagctcggc
    25381 gcccgcgttt ttctggagat ggggcccgat gcccagttgg ttgcttgcgg gcagcgcgaa
    25441 taccgcgata acgcatactg gatagccagc gcccggcgta acaaagaggc gagcgatgtc
    25501 ctcaatcagg ccctgctcca gctttacgct gccggcgtcg ccctaccgtg ggccgacctg
    25561 ctggcgggcg atggacaacg tatcgctgcg ccatgttatc cgtttgatac tgaacgttac
    25621 tggaaagagc gcgtctcccc ggcctgcgag cctgccgacg cagcgctgtc tgccgggctg
    25681 gaggtggcga gtcgcgccgc gacagcgctc gatctccctc gcctggatgc gcttaaacag
    25741 tgcgccacgc gactgcacgc catctacgtc gatcaactgg tacaacgctg taccggcgat
    25801 gccattgaga acggcgtgga cgccatgacc atcatgcgcc gtggacgtct gctgccccgc
    25861 taccagcagc tactccagcg cctgctgaat aactgcgtgg tcgacggcga ttaccgctgc
    25921 accgacgggc gatacgtccg cgcccgcccc attgaacatc aacagcggga atcactgctg
    25981 acggaacttg ccggttattg tgaaggtttt caggctattc ccgacaccat cgcccgtgcc
    26041 ggcgatcggt tatatgaaat gatgagcggc gcggaagaac cggtggcgat tatcttcccg
    26101 caaagcgcct ccgacggcgt ggaagtgctg tatcaggaat tcagctttgg ccgctatttc
    26161 aaccaaatcg ccgccggggt attacgcggc attgtccaga cgcgtcagcc ccgccagccg
    26221 ttgcgtattc ttgaagttgg cggcggaacc ggcggcacca ccgcgtggct gctgccggaa
    26281 ctcaacggcg ttccggcact ggagtaccat ttcaccgata tctcggcgct gttcacccgt
    26341 cgcgcccagc agaaattcgc cgactatgat tttgtgaagt atagcgagct ggatctcgaa
    26401 aaagaggcgc agtctcaggg tttccaggca cagtcttacg atcttatcgt ggcagcgaac
    26461 gtgattcacg ccacccgcca tattggccgc acgctcgata atctgcgccc cctgctcaag
    26521 ccgggcgggc gcctgctgat gcgcgaaatc acccagccaa tgcgtctgtt tgacttcgtt
    26581 ttcggcccgc tggttcttcc gctacaggat ctcgacgccc gcgaaggtga gttattcctc
    26641 accaccgctc agtggcaaca acagtgccgc cacgccggat tcagcaaagt ggcgtggcta
    26701 ccgcaggatg gcagcccgac cgccgggatg agcgaacata tcattctcgc cacgctgccc
    26761 ggtcaggcgg ttagcgccgt aacattcacc gcgccatcag aacccgtgtt ggggcaggcg
    26821 ctgacggata acggtgatta tctcgccgac tggtctgatt gcgcaggtca gcccgaacgg
    26881 tttaacgccc gctggcagga ggcatggcgt ctgctttcac agcgtcatgg cgacgctctg
    26941 cctgtggaac cgccccccgt cgccgccccg gagtggctgg ggaaggttcg cttaagctgg
    27001 caaaacgaag ccttttcccg tggtcagatg cgcgttgaag cccgtcatcc tgctggcgag
    27061 tggctgccgc tatcgcccgc cgcgcctctt cctgcgccgc aaacgcatta tcaatggcgc
    27121 tggacgcccc tcaacgtcgc cagcattgac catccgctta cctttagctt cagcgccggt
    27181 acgcttgcgc gcagcgacga gctggcgcaa tacggcatca ttcacgatcc gcacgcctct
    27241 tcacgactga tgattgttga ggagagcgag gatacgctgg ccttagcgga gaaagtgata
    27301 gcagcgctca ccgccagcgc agccggattg attgtggtta ctcgccgcgc gtggcgagtc
    27361 gaggaaaatg aagcactctc tgcatcccat cacgcgctat gggccttgct tcgcgtcgcg
    27421 gccaacgaac agccggaacg gttgcttgcc gccatcgatc tcgccgaaaa caccccgtgg
    27481 gaaacgctgc atcaagggtt gagcgcagtc tcactatcac agcgctggct cgccgcacgg
    27541 ggtgacaccc tttggctccc ttcactgacg cccaatacgg gatgcgccgc tgaattaccg
    27601 gcaaacgtgt ttaccggcga tagccgctgg catctggtga ccggagcgtt tggcggatta
    27661 ggccgccttg ccgtgaactg gctcagagaa aaaggggcgc gacgcatcgc cctgctggca
    27721 ccgcgcgtgg atgagtcatg gctacgcgac gtggagggcg ggcagacgcg cgtctgccgt
    27781 tgtgatgtgg gcgatgccgg gcaactggcc acggttcttg acgatctggc ggccaacggc
    27841 ggcattgccg gagcgattca tgccgctggc gtattggctg acgcgccctt gcaggagctt
    27901 gatgaccacc agctggctgc cgttttcgcg gtaaaagcgc aggcggcaag ccaactgttg
    27961 caaaccctgc gcaaccacga cggacgctat cttattctct actcttccgc tgccgccacc
    28021 ctcggcgcgc cgggtcagag cgcccatgcg ctggcctgcg gctacctgga cgggctggcc
    28081 cagcagtttt ccacccttga tgcgccgaaa acgctctctg tcgcctgggg cgcatgggga
    28141 gaaagcggtc gggcggccac gccggaaatg ctggcgacgc tcgccagccg aggtatgggc
    28201 gcgttaagcg atgccgaagg ctgctggcac ctggaacagg cggtgatgcg cggcgccccg
    28261 tggcgactgg cgatgcgcgt ttttaccgac aaaatgcccc cgttacaaca ggctctgttt
    28321 aacatcagcg ccacagaaaa agccgcaacg ccggtcattc ctcctgctga tgacaacgcc
    28381 tttaacggca gcctgagcga tgaaacagcg gtgatggcat ggctgaaaaa gcggattgcg
    28441 gttcagctaa ggctgagcga tccggcgtca ctgcatccaa accaggatct gttgcaactc
    28501 ggcatggact cgctgctctt ccttgaactc agtagcgata ttcagcacta cctgggtgta
    28561 cgcatcaatg cggaacgggc gtggcaggat ctgtctcctc atggactcac gcagcttatc
    28621 tgttctaagc cagaggcgac gcctgccgct tcgcagccgg aagtattgcg gcacgacgcc
    28681 gacgagcgtt atgcgccctt ccctttgacg cccattcagc acgcctactg gctggggcga
    28741 acccacctca ttggctatgg cggcgtcgcc tgtcacgtcc tgtttgagtg ggataaacgc
    28801 cacgatgagt tcgatctcgc catactggag aaagcatgga accagctcat cgcacgccac
    28861 gatatgttgc gtatggtggt tgatgccgac gggcagcagc gaatcctggc gacaacgccg
    28921 gagtatcaca tcccgcgtga cgatctgcgc gcgctttccc cggaagaaca gcgcatcgca
    28981 ctggaaaaac ggcggcatga actgagctat cgcgttttgc ctgccgacca gtggcctctt
    29041 tttgagctgg tggtcagcga aatcgacgat tgccattacc gtctgcatat gaacctcgac
    29101 cttttgcagt ttgatgtgca gagttttaaa gtcatgatgg acgacctggc gcaggtctgg
    29161 cgcggtgaaa cgctggcgcc gctcgctatt accttccgtg attatgtgat ggctgaacag
    29221 gcgcgccgac agacatcggc atggcacgat gcctgggatt actggcagga aaaactgccg
    29281 caactgccct tagcgccaga gctgccggtg gttgagacgc ccccggaaac gccacacttc
    29341 accaccttca aatcgacgat cggcaagaca gaatggcagg ccgtgaaaca gcgctggcag
    29401 cagcaaggcg tcacaccgtc tgccgcgctg ctcacgctgt ttgccgccac ccttgagcgc
    29461 tggagccgta ccacaacatt tacgctgaac ctgacgttct tcaatcgcca gccgatccat
    29521 ccgcaaatca accagttgat tggtgatttt acctccgtca cgctggttga ttttaacttc
    29581 tcagcgccgg tgacgttgca agagcagatg caacagaccc aacagcgcct ctggcaaaac
    29641 atggcgcaca gtgaaatgaa cggtgttgag gtgatccgtg agctgggccg cctgcgcgga
    29701 tcacaacgtc aaccgctgat gccggtagtg tttaccagta tgctggggat gacgctggaa
    29761 ggcatgacta tcgatcaggc gatgagccat ctgttcggcg aaccctgcta tgtattcacg
    29821 caaacgccgc aggtctggct ggatcatcag gtcatggaga gcgacggcga gttgatgttt
    29881 agctggtact gcatggacaa cgtgctggaa cccggcgctg ccgaggcgat gtttaatgac
    29941 tattgcgcca tcctgcaagc cgtcatcgcc gcccctgaaa gcctgaagac tctcgccagc
    30001 ggcatcgcca ggcacattcc ccgccgacgc tggccgctga acgcgcaggc ggactacgac
    30061 ctgcgggata ttgagcaggc gacgctcgaa taccccggca tccggcaggc cagagcggaa
    30121 ataaccgaac agggcgcgtt gacgctggat atcgtgatgg ccgacgatcc ttcgccatca
    30181 gcggcgatgc ctgatgagca cgaacttacc caactggcgc tgccgttgcc tgagcaggcg
    30241 cagcttgatg agctggaggc gacctggcgc tggctggagg cgcgtgcgct acaggggatc
    30301 gcggctacgc taaatcgtca cggcctgttt accacgccgg agatcgccca tcgctttagc
    30361 gcagtagtac aggcgctgtc cgcgcaagcg tctcaccagc gtctgctgcg ccagtggcta
    30421 cagtgtctga cggaaagaga gtggttaatc cgcgaaggtg aaagctggcg ctgccgcatt
    30481 ccgctcagcg agattcctga gcctcaggaa gcgtgcccgc aaagccaatg gagccaggcg
    30541 ctggcgcagt atctggaaac ctgcatcgcc cggcacgacg ccctcttctc cgggcagtgt
    30601 tctccgctgg aattgctgtt caacgagcag catcgcgtta ccgacgcgct gtatcgcgac
    30661 aaccccgcca gcgcctgtct gaatcgctat accgcgcaga ttgccgcctt gtgcagcgca
    30721 gaacggattc tggaggttgg cgccggaacc gcagccacta ccgcgccggt gctgaaggcc
    30781 acgcggaaca cgcggcagtc gtaccacttc acggacgtct ccgcgcagtt cctcaatgac
    30841 gccagagccc gtttccatga tgaatcgcag gtgtcttatg ccttgttcga catcaaccag
    30901 ccgctggatt tcaccgccca cccggaggcg ggttacgacc tgatcgttgc cgtcaatgtg
    30961 ctccacgacg ccagccatgt cgtccagacg ttgcgcagat taaaactgtt gctgaaagcc
    31021 ggcggacgtt tgctgatcgt tgaagcgacg gagcgaaaca gcgtattcca gctggcgagc
    31081 gtgggcttta ttgagggatt aagcggatac cgcgatttcc gccgccggga tgagaaaccg
    31141 atgctcaccc gctccgcatg gcaggaggtt ctcgttcagg ccgggtttgc aaacgagctg
    31201 gcgtggcccg cgcaggaatc gtcgccgctg cgccagcatc tgctggtagc gcgttcgcct
    31261 ggcgtaaatc gcccggataa aaaagccgtg agccgctatt tacagcagcg ctttggcacc
    31321 ggtctgccca ttttacagat ccggcaaaga gaagcgttat ttacgccgct gcatgccccg
    31381 tctgatgcgc cgactgagcc agccaaaccc acgccagttg ccggggggaa tccggcactg
    31441 gaaaaacagg tggctgaact ctggcaatcg ctgctgtctc gccccgtggc aaggcatcac
    31501 gactttttcg aactgggcgg cgacagcctg atggcgacaa ggatggtcgc gcagctaaac
    31561 cggagaggga ttgccagggc taaccttcag gatctgttca gccattcgac gctgagcgac
    31621 ttctgcgccc atctacaggc ggctacgtca ggagaggaca acccgatacc cctttgccag
    31681 ggcgacggtg aggaaaccct gtttgtcttc cacgcttcag acggcgatat cagcgcctgg
    31741 ctgccgctcg ctagcgcgtt gaacaggcgc gttttcggcc tgcaagcaaa atcgccgcag
    31801 cgctttgcca cgctcgacca gatgatcgat gagtatgtcg ggtgcatccg tcgtcagcag
    31861 cctcacggcc cttatgtgct ggcgggttgg tcgtatggcg cgtttcttgc ggcgggcgcc
    31921 gcacaacgcc tgtacgccaa aggcgagcag gttcggatgg tgttaatcga tcccgtgtgc
    31981 cgacaggatt tctgttgcga aaaccgggcg gccctgctgc gcctgttagc cgaaggacaa
    32041 acgcctctgg cactgcccga acatttcgac cagcagacgc ccgacagcca gcttgccgac
    32101 tttatcagcc tcgctaaaac ggccggtatg gtgtcgcaaa acctgacgct gcaagcggca
    32161 gaaacgtggc tcgacaacat cgcgcatctg ctgcgtttac tgactgagca tacgccgggc
    32221 gaaagcgttc cggtcccctg tctcatggtg tatgccgccg ggagacccgc gcactggacg
    32281 ccagcagaaa ccgagtggca gggctggata aacaacgccg acgacgctgt gattgaagcc
    32341 agccactggc aaatcatgat ggaagccccc cacgttcagg cttgtgcgca acacattacg
    32401 cgctggcttt gcgcaacctc aacgcaaccg gagaacacgt tatgatgccg tccgcctccc
    32461 caaaacaacg cgtactgatt gtgggcgcca aatttggcga aatgtacctg aatgccttta
    32521 tgcagccccc ggaggggctg gaactggtcg gcctgctggc gcagggaagc gcccgttcaa
    32581 gagagctggc tcatgcgttt ggcattccgc tgtatacctc gccggaacag ataaccagga
    32641 tgccggatat cgcctgtatt gtcgtgcgtt cgaccgtcgc cggaggaacc ggtacgcagc
    32701 ttgccagaca ctttctgacg cgcggcgtac acgtcattca ggagcatccc ctccacccgg
    32761 atgacatcag ttccttacaa acgctggcgc aggaacaggg ctgctgctat tgggtaaaca
    32821 cgttttatcc gcatacgcgc gcgggacgca catggcttcg cgatgcgcag cagctgcgtc
    32881 gctgtctggc taagacgccg caggtggtcc acgccaccac cagccgacag ttgctctact
    32941 ccactctgga tttgttgttg ctggctctgg gcgttgatgc cgccgccgtg gagtgcgacg
    33001 tggttggcag ctttagcgat tttcactgtc tgagactctt ctggccggag ggagaagcct
    33061 gcctgctgct tcagcgctat ctcgatcctg acgatccaga tatgcatagc cttatcatgc
    33121 accgcctgct gctgggctgg ccggaaggtc atctttcgct ggaggcgagc tacggccccg
    33181 tcatctggtc atccagcctg tttgtcgcag accatcagga gaacgcccac agcctctacc
    33241 gcagaccgga gatcctgcgc gatctgccgg gtctgacgcg cagcgcggcg cccctgagct
    33301 ggcgcgactg ctgcgaaacc gtcgggccgg agggcgtcag ctggttgcta caccagttgc
    33361 gcagccatct ggcgggcgaa catccaccag cggcctgcca aagcgtacat cagatcgcgt
    33421 tatcacgttt atggcagcag attttacgta agaccggcaa tgcggagatc agacgcctga
    33481 cgccgccgca ccacgaccgg ttagccggtt tctacaacga tgatgataag gaggcgctgt
    33541 gacgcaatct gcaatgtgca tcccgctgtg gcccgcccgg aacggcaata ctgcgcatct
    33601 ggtcatgtgc cctttcgctg gcggcagcag tagcgcgttt cgccactggc aagctgagca
    33661 actggctgat tgcgcgcttt ctctggtgac ctggccgggg cgcgatcgcc ttcgccatct
    33721 ggaaccgctc agaaacatta cacaactggc ggcactgctg gcgaacgagc tggaagcatc
    33781 cgtatcgcct gacacgccgc ttttactcgc cgggcacagc atgggggcgc aggtggcgtt
    33841 tgaaacctgc cgacttctgg agcaacgggg gcttgcgcca caaggactga ttatttccgg
    33901 gtgccatgcc ccgcatctgc attctgaacg ccagctcagc catcgcgatg atgccgactt
    33961 tatcgctgag ctgatagaca ttggcggatg ttctcctgaa ctgcgggaaa accaggaatt
    34021 aatgtcgctg tttcttcctc ttctgcgcgc tgatttttac gccaccgaga gctatcacta
    34081 cgactcgccc gacgtctgtc cgccgctgcg cacgcctgcg ctgttattgt gcggcagcca
    34141 cgatcgcgaa gcctcctggc agcaggtcga tgcctggcgt cagtggctga gccacgttac
    34201 aggcccggtg gtgattgacg gcgatcattt ctatcccatt caacaagccc ggtccttttt
    34261 tacgcagatt gtccgccatt ttccccacgc attttctgca atgaccgcgt ggcaaaaaca
    34321 gcccagtact tcagaaaggt gacgcatgaa ttcttccttt gaatctctga ttgaacagta
    34381 tcccttaccc attgccgaac agttgcgcca ctgggcggcc cgttatgcct cgcgaattgc
    34441 cgtcgttgat gcaaaggggt cgttaaccta cagcgcgctt gatgcacaag ttgacgaact
    34501 tgccgcaggt ctgtcatcac tgggtttgcg ttcgggggag catgtaattg tgcagcttcc
    34561 caacgacaac gcgtttgtta ccctgctgtt cgccttgtta agactgggcg ttatccccgt
    34621 gctggcgatg ccctcgcaac gggctctgga tatcgacgcg ctgattgagc tggcgcaacc
    34681 cgtcgcttac gttattcacg gggaaaacct cgcagagctg gcccgacaga tggcgcacaa
    34741 acacgcctgc ttgcgtcatg ttctggtcgc tggagagacc gtgagcgacg attttacgcc
    34801 gctcttctcc cttcacggtg agcgacaggc atggccgcag cctgatgttt ccgccaccgc
    34861 gttgttgttg ctctcaggcg gcacaaccgg cacgcccaaa ctcatcccgc gccgacatgc
    34921 cgactatagc tataacttca gcgcttctgc tgaactgtgc ggcatcagcc aacagagcgt
    34981 gtatctcgcc gtcctcccgg tggcgcataa ctttccgctg gcctgccccg gtattctggg
    35041 aacgcttgcc tgcggcggaa aagtggtgct gaccgacagc gccagctgtg atgaggtgat
    35101 gcctttaatc gcgcaggaaa gagtgactca cgtcgccctg gttccggcgc tggcgcaatt
    35161 atgggtgcag gccagggagt gggaagacag cgacctttcg tcgctgcgcg tcattcaggc
    35221 aggcggcgcc cggctcgacc cgacgcttgc tgagcaggtt atcgccacct ttgactgtac
    35281 cctgcaacag gttttcggta tggcggaagg cctgctctgt tttacccggc tggacgatcc
    35341 gcatgccacc attctccaca gccaggggcg cccgttgtcc cctctggatg aaatccgcat
    35401 cgttgatcaa gacgagaacg acgtcgcgcc gggcgaaacc gggcaattgt taacgcgcgg
    35461 cccttatacc atttcgggct attaccgcgc ccctgcccac aacgcgcagg cctttaccgc
    35521 gcaagggttt taccgcacag gcgacaatgt caggctggat gaggtgggga acctgcacgt
    35581 tgagggacgc ataaaagagc agatcaaccg cgccggagaa aaaatagccg cggctgaagt
    35641 ggaatcggca ctgctgcgtt tagcggaagt gcaagattgc gcggtggtcg ccgcgccgga
    35701 cacgctgctt ggcgagcgga tttgcgcgtt tatcatcgcg cagcaggtgc caactgacta
    35761 tcagcagttg cgtcaacaac tgacccgtat ggggctcagc gcgtggaaaa ttcctgacca
    35821 aatcgagttt ctggaccact ggccgctcac cgccgtcggc aagatagaca aaaaacgcct
    35881 gacggctctc gccgtcgacc gttatcgcca ttctgcccaa taagcgcaaa ccgacccgaa
    35941 acaggttgaa ataaacccgt ttcgggtagc accactatta gaaatagtta tcattttcaa
    36001 ttcaccattg tcggtatttt tggcgtttcg ccgtcttaca gggactcaca acaatgaaaa
    36061 tgacacggct ttatcctctg gccttggggg gattattgct ccccgccatt gctaatgccc
    36121 agacttcaca gcaagacgaa agcacgctgg tggttaccgc cagtaaacaa tcttcccgct
    36181 cggcatcagc caacaacgtc tcatctactg ttgtcagcgc gccggaatta agcgacgccg
    36241 gcgtcaccgc cagcgacaaa ctccccagag tcttgcccgg gctcaatatt gaaaatagcg
    36301 gcaacatgct tttttcgacg atctcgctac gcggcgtctc ttcagcgcag gacttctata
    36361 accccgccgt caccctgtat gtcgatggcg tccctcagct ttccaccaac accatccagg
    36421 cgcttaccga tgtgcaaagc gtggagttgc tgcgaggccc acagggaacg ttatatggca
    36481 aaagcgctca gggcgggatc atcaacatcg tcacccagca gccggacagc acgccgcgcg
    36541 gctatattga aggcggcgtc agtagccgcg acagttatcg aagtaagttc aacctgagcg
    36601 gccccattca ggatggcctg ctgtacggca gcgtcaccct gttacgccaa gttgatgacg
    36661 gcgacatgat taaccccgcg acgggaagcg atgacttagg cggcacccgc gccagcatag
    36721 ggaatgtgaa actgcgtctg gcgccggacg atcagccctg ggaaatgggc tttgccgcct
    36781 cacgcgaatg tacccgcgcc acccaggacg cctatgtggg atggaatgat attaagggcc
    36841 gtaagctgtc gatcagcgat ggttcaccag acccgtacat gcggcgctgc actgacagcc
    36901 agaccctgag tgggaaatac accaccgatg actgggtttt caacctgatc agcgcctggc
    36961 agcagcagca ttattcgcgc accttccctt ccggttcgtt aatcgtcaat atgcctcagc
    37021 gctggaatca ggatgtgcag gagctgcgcg ctgcaaccct gggcgatgcg cgtaccgttg
    37081 atatggtgtt tgggctgtac cggcagaaca cccgcgagaa gttaaattca gcctacgaca
    37141 tgccgacaat gccttattta agcagtaccg gctataccac cgctgaaacg ctggccgcat
    37201 acagtgacct gacctggcat ttaaccgatc gttttgatat cggcggcggc gtgcgcttct
    37261 cgcatgataa atccagtaca caatatcacg gcagcatgct cggcaacccg tttggcgacc
    37321 agggtaagag caatgacgat caggtgctcg ggcagctatc cgcaggctat atgctgaccg
    37381 atgactggag agtgtatacc cgtgtagccc agggatataa accttccggg tacaacatcg
    37441 tgcctactgc gggtcttgat gccaaaccgt tcgtcgccga gaaatccatc aactatgaac
    37501 ttggcacccg ctacgaaacc gctgacgtca cgctgcaagc cgcgacgttt tatacccaca
    37561 ccaaagacat gcagctttac tctggcccgg tcgggatgca gacattaagc aatgcgggta
    37621 aagccgacgc caccggcgtt gagcttgaag cgaagtggcg gtttgcgcca ggctggtcat
    37681 gggatatcaa tggcaacgtg atccgttccg aattcaccaa tgacagtgag ttgtatcacg
    37741 gtaaccgggt gccgttcgta ccacgttatg gcgcgggaag cagcgtgaac ggcgtgattg
    37801 atacgcgcta tggcgcactg atgccccgac tggcggttaa tctggtcggg ccgcattatt
    37861 tcgatggcga caaccagttg cggcaaggca cctatgccac cctggacagc agcctgggct
    37921 ggcaggcgac tgaacggatg aacatttccg tctatgtcga taacctgttc gaccgtcgtt
    37981 accgtaccta tggctacatg aacggcagca gcgccgtcgc gcaggtcaat atgggtcgca
    38041 ccgtcggtat caatacgcga attgatttct tctgattatt gtaaaaggga taccgaaaag
    38101 gtatcccttt tacaccacta gttaaaacca gtaactcagc agagt
//
LOCUS       DAPQZD010000029        38011 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPQZD010000029 DAPQZD010000000
VERSION     DAPQZD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.029"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            145..771
                     /locus_tag="RO454_001428"
     CDS             145..771
                     /locus_tag="RO454_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDY0933278.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            792..2894
                     /locus_tag="RO454_001429"
     CDS             792..2894
                     /locus_tag="RO454_001429"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDY0933279.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2907..3545
                     /locus_tag="RO454_001430"
     CDS             2907..3545
                     /locus_tag="RO454_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDY0933280.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3609..4277
                     /locus_tag="RO454_001431"
     CDS             3609..4277
                     /locus_tag="RO454_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDY0933281.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4274..5059
                     /locus_tag="RO454_001432"
     CDS             4274..5059
                     /locus_tag="RO454_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDY0933282.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5063..5875
                     /locus_tag="RO454_001433"
     CDS             5063..5875
                     /locus_tag="RO454_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933283.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5887..7491)
                     /locus_tag="RO454_001434"
     CDS             complement(5887..7491)
                     /locus_tag="RO454_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDY0933284.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7617..7922)
                     /locus_tag="RO454_001435"
     CDS             complement(7617..7922)
                     /locus_tag="RO454_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDY0933285.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(8031..8107)
                     /locus_tag="RO454_001436"
     tRNA            complement(8031..8107)
                     /locus_tag="RO454_001436"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8071..8073),aa:Val,seq:gac)
     gene            complement(8112..8188)
                     /locus_tag="RO454_001437"
     tRNA            complement(8112..8188)
                     /locus_tag="RO454_001437"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8152..8154),aa:Val,seq:gac)
     gene            8496..9752
                     /locus_tag="RO454_001438"
     CDS             8496..9752
                     /locus_tag="RO454_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDY0933286.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9793..11166)
                     /gene="mdtK"
                     /locus_tag="RO454_001439"
     CDS             complement(9793..11166)
                     /gene="mdtK"
                     /locus_tag="RO454_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDY0933287.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11381..12022
                     /gene="ribE"
                     /locus_tag="RO454_001440"
     CDS             11381..12022
                     /gene="ribE"
                     /locus_tag="RO454_001440"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDY0933288.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12062..13210)
                     /gene="cfa"
                     /locus_tag="RO454_001441"
     CDS             complement(12062..13210)
                     /gene="cfa"
                     /locus_tag="RO454_001441"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDY0933289.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13501..14712)
                     /locus_tag="RO454_001442"
     CDS             complement(13501..14712)
                     /locus_tag="RO454_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDY0933290.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14825..15757
                     /locus_tag="RO454_001443"
     CDS             14825..15757
                     /locus_tag="RO454_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0933291.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15754..16779)
                     /gene="purR"
                     /locus_tag="RO454_001444"
     CDS             complement(15754..16779)
                     /gene="purR"
                     /locus_tag="RO454_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDY0933292.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17078..17167
                     /gene="ynhF"
                     /locus_tag="RO454_001445"
     CDS             17078..17167
                     /gene="ynhF"
                     /locus_tag="RO454_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDY0933293.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17333..18502
                     /locus_tag="RO454_001446"
     CDS             17333..18502
                     /locus_tag="RO454_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933294.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18648..19229)
                     /gene="sodB"
                     /locus_tag="RO454_001447"
     CDS             complement(18648..19229)
                     /gene="sodB"
                     /locus_tag="RO454_001447"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDY0933295.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19357..20184)
                     /gene="mepH"
                     /locus_tag="RO454_001448"
     CDS             complement(19357..20184)
                     /gene="mepH"
                     /locus_tag="RO454_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDY0933296.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20519..20866
                     /gene="grxD"
                     /locus_tag="RO454_001449"
     CDS             20519..20866
                     /gene="grxD"
                     /locus_tag="RO454_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDY0933297.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20917..21009)
                     /locus_tag="RO454_001450"
     CDS             complement(20917..21009)
                     /locus_tag="RO454_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDY0933298.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(21238..21885)
                     /gene="rnt"
                     /locus_tag="RO454_001451"
     CDS             complement(21238..21885)
                     /gene="rnt"
                     /locus_tag="RO454_001451"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDY0933299.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(21988..22395)
                     /gene="gloA"
                     /locus_tag="RO454_001452"
     CDS             complement(21988..22395)
                     /gene="gloA"
                     /locus_tag="RO454_001452"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDY0933300.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22476..23573)
                     /gene="nemA"
                     /locus_tag="RO454_001453"
     CDS             complement(22476..23573)
                     /gene="nemA"
                     /locus_tag="RO454_001453"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDY0933301.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(23610..24209)
                     /gene="nemR"
                     /locus_tag="RO454_001454"
     CDS             complement(23610..24209)
                     /gene="nemR"
                     /locus_tag="RO454_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDY0933302.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24312..24551
                     /locus_tag="RO454_001455"
     CDS             24312..24551
                     /locus_tag="RO454_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDY0933303.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            24600..25496
                     /locus_tag="RO454_001456"
     CDS             24600..25496
                     /locus_tag="RO454_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDY0933304.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25577..26098
                     /gene="sodC"
                     /locus_tag="RO454_001457"
     CDS             25577..26098
                     /gene="sodC"
                     /locus_tag="RO454_001457"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDY0933305.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26099..28111)
                     /locus_tag="RO454_001458"
     CDS             complement(26099..28111)
                     /locus_tag="RO454_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDY0933306.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28111..28968)
                     /locus_tag="RO454_001459"
     CDS             complement(28111..28968)
                     /locus_tag="RO454_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDY0933307.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(28971..29207)
                     /locus_tag="RO454_001460"
     CDS             complement(28971..29207)
                     /locus_tag="RO454_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDY0933308.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29408..29842
                     /gene="slyA"
                     /locus_tag="RO454_001461"
     CDS             29408..29842
                     /gene="slyA"
                     /locus_tag="RO454_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDY0933309.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29889..30356)
                     /gene="slyB"
                     /locus_tag="RO454_001462"
     CDS             complement(29889..30356)
                     /gene="slyB"
                     /locus_tag="RO454_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDY0933310.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30630..31739
                     /gene="anmK"
                     /locus_tag="RO454_001463"
     CDS             30630..31739
                     /gene="anmK"
                     /locus_tag="RO454_001463"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDY0933311.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31837..32166
                     /gene="mliC"
                     /locus_tag="RO454_001464"
     CDS             31837..32166
                     /gene="mliC"
                     /locus_tag="RO454_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDY0933312.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32225..32881
                     /gene="pdxH"
                     /locus_tag="RO454_001465"
     CDS             32225..32881
                     /gene="pdxH"
                     /locus_tag="RO454_001465"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDY0933313.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33010..34284
                     /gene="tyrS"
                     /locus_tag="RO454_001466"
     CDS             33010..34284
                     /gene="tyrS"
                     /locus_tag="RO454_001466"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDY0933314.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34346..35206
                     /gene="pdxY"
                     /locus_tag="RO454_001467"
     CDS             34346..35206
                     /gene="pdxY"
                     /locus_tag="RO454_001467"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDY0933315.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35250..35855)
                     /gene="gstA"
                     /locus_tag="RO454_001468"
     CDS             complement(35250..35855)
                     /gene="gstA"
                     /locus_tag="RO454_001468"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDY0933316.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(35961..37463)
                     /gene="dtpA"
                     /locus_tag="RO454_001469"
     CDS             complement(35961..37463)
                     /gene="dtpA"
                     /locus_tag="RO454_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDY0933317.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         9727 a         9130 c         9862 g         9292 t
ORIGIN      
        1 gttagcgtta aatcttttct catacactat cgggttaggg aaaatatctg gtgttgagga
       61 cagcgtcagc agcgtattag ttgagtgctt aatatcaggc tgttgaattg tcagcactgt
      121 gtttgcgaag aggatgcgaa ataaatgaac ccggttgatc gtccactatt aaatattggc
      181 ttaacgcgat tagaattctt gcgtatatca ggaaaaggcc ttgctggttt aacaattgcc
      241 cctgctttac tgtcactttt gggttgcaaa caggaagata ttgatagcgg cacagtaggg
      301 ttgataaata cacccaaagg ggtgctggtg acccaacgtg cgcgatgcac gggttgccac
      361 cgctgtgaaa tctcttgtac caacttcaac gatggctcag tagggacatt cttctcccgt
      421 atcaaaatcc atcgcaatta tttctttggc gacaacgggg ttggctctgg cggtggcctg
      481 tatggcgatc tcaactatac cgcggatacc tgccgtcaat gcaaagagtc gcaatgcatg
      541 aacgtctgcc cgattggtgc gattacctgg cagcagaaag aaggctgtat taccgtcgat
      601 cataaacgtt gtattggctg tagcgcctgt actacggcat gcccgtggat gatggccacc
      661 gtaaataccg aaagtaaaaa atcctcgaaa tgtgtgttat gcggtgaatg cgcaaacgcc
      721 tgcccaacag gggcgttaaa aattatcgag tggaaagata ttactgtgtg aatcttgcaa
      781 aggaaaaaat catggctaac ggttggacag gtaatatatt aagagtcaat ctcacgacag
      841 gaaatattac cctcgaagat tccagtaaat ttaaaagttt tgtcggtggc atgggcttcg
      901 gctacaaaat tatgtatgac gaagtaccgc caggcacgaa acctttcgat gaagcgaata
      961 aattagtttt tgctaccggc ccattaactg gatctggtgc cccctgtagt tctcgcgtaa
     1021 atatcacctc actttctact tttaccaaag gaaatttagt cgtcgatgcc catatggggg
     1081 gcttttttgc agcgcaaatg aaattcgctg gatacgacgt cattattatc gaagggaaag
     1141 cgaaatcacc ggtatggctg aagattaaag atgacaaagt tagccttgaa aaagccgatt
     1201 tcttatgggg aaaagggacg cgcgcaacga cggaagaaat ttgtcgcttg accagtccgg
     1261 aaacctgtgt ggcggctatt ggtcaggctg gagaaaacct tgttcctctc tctggcatgt
     1321 tgaatagccg taaccacagc ggcggtgcgg gaactggcgc aataatgggt tcgaaaaacc
     1381 tgaaagcgat tgcggttgaa gggacgaaag gggtcaacat tgccgatcgt caggagatga
     1441 agcgtctcaa cgattacatg atgactgaac ttatcggtgc gaataacaac catgtcgtgc
     1501 caagtacgcc gcaatcgtgg gcagagtatt cagatcccaa gtcacgttgg acagcacgta
     1561 aagggctgtt ttggggcgcg gctgaaggtg gtccgattga aacgggggaa attccgccag
     1621 gcaatcagaa tacggtcggc tttcgtacct ataaatccgt ttttgactta ggtccggcgg
     1681 cagagaaata cacagtaaaa atgagtggct gccactcttg cccgatccgt tgtatgaccc
     1741 aaatgaatat tcctcgggtg aaagagtttg gcgtgcccag cacaggtggt aacacttgtg
     1801 tagcaaactt tgtccatacc accatcttcc cgaacgggcc gaaagatttt gaagataagg
     1861 acgatggtcg tgtgattggt aacctggttg gtctgaattt gttcgatgac tacggcctat
     1921 ggtgtaacta cgggcagttg catcgcgact ttacttattg ttacagcaaa ggtgtgttca
     1981 agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg gaagcgggtg
     2041 acgttaactt tattaaagat ttttactacc gtctggcgca tcgcgtgggt gagttgagtc
     2101 acctggctga tggttcatat gccatcgcag aacgctggaa tttgggtgaa gagtactggg
     2161 gctacgcgaa aaataaactc tggtcgccgt ttggctatcc ggttcaccat gccaatgaag
     2221 cgtcagcgca agtcggttcc attgttaact gtatgttcaa ccgtgactgc atgacgcata
     2281 cccatatcaa ttttattggt tccggcttgc cattgaaact gcagcgtgaa gtggcgaaag
     2341 agctttttgg ttctgaagat gcttacgatg aaaccaaaaa ctacacaccg atcaatgacg
     2401 caaaaattaa atatgctaag tggtcgctgt tgcgggtctg tttgcataac gccgtcaccc
     2461 tgtgcaactg ggtctggcca atgaccgttt cgccgctgaa aagccgtaat taccggggtg
     2521 atctggcgct tgaagccaaa ttcttcaaag cgatcactgg cgaagatatg actcaggaaa
     2581 aattagattt agctgcagag cgtatcttta cgttgcatcg cgcctacacg gtaaaactga
     2641 tgcaaaccaa agatatgcgt aatgaacacg atcttatctg ttcctgggta ttcgacaagg
     2701 atccgcagat cccggtcttt actgaaggta cagacaaaat ggaccgtgac gatatgcatg
     2761 cttcgctcac gatgttctac aaagaaatgg gctgggaccc acagcttggt tgtccgaccc
     2821 gcgaaacatt gcagcgtctg gggctggaag atattgccgc cgatctggca gcacacaatc
     2881 tactgcctgc gtaaggaatg ggagagatga accatcagga tgaattgccg ttagcgaaag
     2941 tcagcgaaat tgatgaggcg aagcggcaat ggttgcaggg gatgcgtcac cccgttgata
     3001 cggtaaccga accagaaccg gctgaaatcc tggcagagtt cattcgccag cattctgcgg
     3061 ctgggcagct ggtggcgcgc gccgtgtttc tctcgccacc ctattcggtt gcagaagagg
     3121 aactgtcagt tttgctggaa aacattaagc agaacggaga ctacgcggat atcgcttgta
     3181 tgacgggaag ccaggatgat tactattact caacgcaggc catgagtgaa aactacgcgg
     3241 cgatgtcttt gcaagttgtg gagcaggaca tttgtcgcgc cattgcacac gccgttcgtt
     3301 ttgagtgcca gacgtatcct cgtccttaca aagtcgccat gctgatgcaa gcgccatatt
     3361 acttccagga agcgcagatt gaagccgcga tcgccgcgat ggatgttgca cctgaatatg
     3421 ccgatatacg gcaggtggag tcatcgacgg cagtgttgta cttgtttagc gagcgattta
     3481 tgacctatgg caaggcgtat ggcttatgcg aatggttcga ggtagagcag tttcagaacc
     3541 cctgaaattt cagggggatg tcatggatag ggtggacagt tgcagccact gcattagggg
     3601 ataatcagat gtccttcact cgacgcaaat ttgttctggg gatgggaaca gtaatctttt
     3661 ttactggttc tgcgtcttct ctgttagcga atacgaggca agaaaaggaa gttcggtatg
     3721 ccatgattca tgacgagtca cggtgcaatg gctgtaatat ctgcgcccgc gcatgccgta
     3781 aaactaacca cgtccccgct cagggaagcc gtttatcgat agcacacatt ccagtcatcg
     3841 ataacgacaa tggaacgcag tatcactttt ttcgtcagtc atgtcagcac tgcgaagatg
     3901 ctccttgcat cgacgtttgc ccgacagggg cgtcgtggcg tgatgagcag gggatcgtgc
     3961 gggtagaaaa atcgcagtgc atcggctgca gttattgcat tggcgtttgc ccgtaccagg
     4021 ttcgctacct taaccctgtc actaaagtgg cagataaatg tgatttctgc gctgagaccc
     4081 gactggcaaa aggctttccg cccatttgcg tcagtgcctg cccggaacac gcattgatct
     4141 ttggtcacga agatagccca gaaattcagg cgtggttgca ggaaaataaa tactatcaat
     4201 atcagctacc tggcgccggg aaaccgcatc tgtatcgccg gttcggtcaa catttgatta
     4261 aaaaggaaaa tgtatgaacc cgtcgcaaca tgctgaacag tttcagagcc agttggcgaa
     4321 ctatgtgcca cagttcaccc cccaattttg gccggtgtgg ttgatcattg ccggactatt
     4381 gctggttggg atgtggttgg tgctggggct acatgccttg cttcgtgctc gtggcgtgaa
     4441 gaaatcagcc accgatcata gtgagaggat ttatctttac agcaaagcgg tcagattatg
     4501 gcactggtcg aatgcgttac tctttgtatt gttgctggca agtgggctga taaatcactt
     4561 tgcgctggtg ggcgcaactg cggttaaaag tctggttgcg gtgcatgaag tttgcggatt
     4621 tttgttactg gcatgctggc ttggctttgt tctgatcaat gccgttgggg gtaatggtca
     4681 ccactatcgc attcgtcgtc agggttggct ggatcgagcg gcaaaacaaa cgcgatttta
     4741 tttgtttggc attatgcagg gggaagaaca tcctttcccg gcaacaaccc agtctaaatt
     4801 taatccctta cagcaggtcg cctatgttgg tgtcatgtat ggattgttgc cattgttact
     4861 attgacgggg ctgctgtgtc tctatccgca agccgtggga gatgtgtttc ctggcgtaag
     4921 atactggtta ttgcaggcgc attttgctct ggcatttata agcctctttt ttatcttcgg
     4981 tcatctttat ctttgcacca cggggcgtac gccacacgaa acctttaaaa gcatggtcga
     5041 tggctatcac cggcactaac gaatgatcat tacacgagca gacctgcgag aatggcgtat
     5101 cggagcggtg atgtatcgct ggtttctgcg ccatttccca cgtggcggta gttatgccga
     5161 tattcatcat gcactgattg aggaaggata tacggactgg gcggagagtc tggttgagta
     5221 tgcgtggaaa aaatggctgg cagatgaaaa cttcgcgcat caggaagttt cctctatgca
     5281 aaaactggcc acagatcccg gagaacgact attttgcagt cagtttgtcc gcagcgacga
     5341 tcatgctcgc ctcggctgtt gtgaggataa cgccagaatc gcaaccgctg ggtatgccgc
     5401 gcaaattgcc agtatgggtt atagcgtgcg gattggaagt gtcggcttta acagtcacat
     5461 tggcagctcc ggtgcacgcg cacgggttgc tgtaaccggt aattcttcgc ggataagcag
     5521 cgctggagat agcggtcgga ttgcgaatac cggaatgcga gtacgcgttt gtacattggg
     5581 tgaacggtgt catgtcgcca gtaatggtga cctggtgcaa atcgccagct ttggcgcaaa
     5641 cgccagaatt gccaacagcg gcgacaatgt acacatcatt gccagtggtg aaaattcaac
     5701 ggtagtgagc acgggcgtgg tcgactcaat tattctcggg ccagggggat gcgccgcact
     5761 ggcatatcat gacggcgaaa gagttcgctt cgccgtcgcc attgaaggtg aaaacaatat
     5821 tcgagccgga gtccgctatc gtcttaatga acaacatcag tttgttgagt gctgatagct
     5881 ttacctttaa tcaaacgaaa gacgccgacg cgcacgggat gcaggtttta cgaccgcccg
     5941 agccatcgcc tctccaattt ctgcacatgc cgtaattccc tgaacgaaag gctggtcgtg
     6001 catcaaccag ggcaacgcgc cgaacgctac gcgcccacga atatcttcgg gttgctgtaa
     6061 cgtataatct tcgccaacat tagggatttc atcccctgtt ttctgtaatt gttcgcgtag
     6121 tccagggaaa ggaatatctt tcactttaag cggacgctgc ccgcgggcat caataaaaac
     6181 gtcaaacgag tagctattgt cttccgtttt caggacggtg cgcgactcat taatttccat
     6241 ttcgtagtct tcaccgaggg cgagaatatg aatgattccc gcttcacgta aggccagtag
     6301 tcgacgaata gactctgaag ggattgccgc ataattatcg atgaaaaccc gggcaaggcc
     6361 tttactgaac cgtttatggt cgtgttcatt cagatgtgga acaatttcct gtacggcttc
     6421 atgcaggcgc agaatgacat aacgccaggg aacagtatgt ttttctcgtt tattgcgttc
     6481 gacttcctgg agattttctt ctgcccagtc aaatgggtcg cgttgtttgc gctcggcaaa
     6541 ccaggcttga gcaaaggaat cgacattcag gctctctaag gctatgcgtt ggctccagtc
     6601 tggatcagca aacttgattt cctctactat caatctaaat acccgatcca aaaggcccac
     6661 ttcgcctttt tgaatctcag catttaatgc ctgatcggtg atgatgtgta agggctcgta
     6721 gggaataggg caatagaaat cggcttcggg taaaatacct gtgcgcgaca ttaacgtaat
     6781 atttagcttt tcatttgcgt tatcgcggtg aaaagtgacg tgttgtttat catcttcaat
     6841 gaacgaacca tgctgaatag ccactgccat tgccgcatcc agtccgctca aggatgttcc
     6901 cataataccc acgttacacg catcgacctt tgcttccatc aggcctgacc acgggctggg
     6961 aaaatacgtt ctggttgctt cttcttcatc aggccagacg tgccccgttg cgatcaccgc
     7021 taaatcaaac gtctcgctgg gtaaattcag atttgtagcg agcatgacgc cagcatttgt
     7081 aatttgcaga tcggtaacct ggcatgattc ataaacagcc actgcaaatt tttgctgtcg
     7141 tgcctggtct actaatcgta aaaattgatc gcggaaatat tcgcccagca gaattcgcgg
     7201 taaaaactga cgatcgtgca aggtttcttt tttaacgcca taacgctgga gatggctggc
     7261 ttcttgcttt tgtagccatt cgagatacgt gcaataaatc ggcggtattt caatactggc
     7321 aatgtttgcc agcatcagtt ttgagttttc ctcatcactg tatggcatcc cgataccggc
     7381 ttcgtcagcc tgatcgaaga tagaaataga aagtggcgtt tgttgctgta gaagcgagaa
     7441 taaggtgtag atccccgtag gcccggcacc cacaatagca attgttttca ttgttactta
     7501 cctgtacgca ttctttcgaa tttagtgtta tcaggtaagt ttggttgaga tttttagaat
     7561 ttgagaaatt tcagacaggg atgaaaaggg cactaaccgc ccttgattaa aatctgtcag
     7621 gcaagttttg cctgattgat ttgactcagt ggcacattga catcgaaaac tttggcgaca
     7681 acttcctcaa cgccgagatt tttcagtcgg gcagtatgtt tttccagata ggcaagggcg
     7741 cttttttcat cagtgaaaag gtagatccca ccggcttcgt ggttcttttc actttctgtc
     7801 caaaccttcc acagaaaacc aggttcctga ttaatcgact cagcaagcgg tttaagctgc
     7861 tcagccattg tgtcgccaaa tgggccattg aaagaaaaat gaagttgtaa caaagttgcc
     7921 atagcgattc tccttctgat aacgcatacg aagacagtgt aaatctttta acgtattgga
     7981 tacagagaag aaacagaggg aaaaaaggag aagaccagat taagaaaatc tggtgcgtcc
     8041 gagtggactc gaaccaccga cccccaccat gtcaaggtgg tgctctaacc aactgagcta
     8101 cggacgcagg atggtgcgtt caattggact cgaaccaacg acccccacca tgtcaaggtg
     8161 gtgctctaac caactgagct atgaacgcaa cgttgtaggt gacaacgggg acgaatatta
     8221 gcggcagagt gggaaggtgg caagaggcaa aacgtaattt tctgcgctat ttcgaccgtt
     8281 tgcagagctt ttaagcaaat tggctatatt ttgttgattt gcaagggcga tttttattca
     8341 ggatcgcatt tacatctgat acaacccaat aatccgtgta tcagagttta aacagagttg
     8401 tttgccacca tctcaaatct gtcgtggata aatccttcgt agcctttaca cttaggtagc
     8461 aataaaggca cacagatgca gcaaaggaat aaatcatggg atctgatgcg aaaaacttga
     8521 tgagcgacgg gaacgtgcaa attgttaaga ccggcgaggt tattggcgcg acgcaactta
     8581 ctgaaggcga gttgattgtt gaagctggcg gaagagccga aaataccgtg gtcacggggg
     8641 ctggctggtt gaaagtggca accggtggga tagccaaatg cacacagtac ggtaacaatg
     8701 gcacactatc ggtcagcgac ggtgccattg ccacagatat tgttcagtcc gagggaggcg
     8761 caattagtct ctctacgctc gctacggtta atggccgcca tcccgaaggt gaattcagcg
     8821 ttgataaagg ttatgcctgc ggtttgttgc tggaaaatgg cggtaacctg cgtgtactgg
     8881 aaggccatcg cgcggaaaaa attattctcg atcaagaggg tggcctgttg gtcaatggga
     8941 caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt ggtgtatcca ggtggggaag
     9001 cctgcaattg tgagattaat cagggcggcg tttttatgct ggcggggaaa gccagtgata
     9061 cgttgcttgc tggtggcacc atgaataatc tcggtggtga agactctgac actattgttg
     9121 agaatggagc catctatcgt ctggggacgg atggtcttca gctctacagt tccggtaaga
     9181 cgcaaaacct gtccgttaat gtgggtggtc gggctgaagt gcatgccggt acgctggaaa
     9241 atgcggtaat acaaggtggg acagtgatcc tgttgtcacc caccagcgcg gacgaaaatt
     9301 ttgtcgtaga ggaagatcgc gcaccggttg aactgaccgg tagtgttgca ttactggacg
     9361 gcgcttcaat gattattggc tatggcgcag atctgcaaca atcaacgatt actgtacagc
     9421 agggcggtgt attgattctc gacggcagta cggtaaaagg tgacagtgtc acttttggta
     9481 ttggtaacat caatctaaat ggcggaaaac tgtggctgat cactgatgcg gcaacgcatg
     9541 tgcaactgaa agtgaaacgc ctgcgcggag agggagccat ttgcctgcaa accagtgcga
     9601 aagaaatttc acctgacttc atcaatgtga aaggggaagt tactggtgat atacacgttg
     9661 agataacaga tgccagtcgg caaactctgt gtaacgcact gaaactacag ccagacgaag
     9721 acgggattgg cgcaacgctc cagcctgcgt aaaaacacag gcgcaacccg tgaaggttgc
     9781 gccttgtctt tactagcgag ctgctcgttg cagaatgatg gctgacggca gacgttgcag
     9841 gaaccgcata cgcagcatca tcataatggc tgccgaagtc aggccaataa taaagcctat
     9901 ccagaagcct gctggcccca taggttcaac gaccagatcg gtcagtgcca gaatatagcc
     9961 gcttggcaag cccagcaccc agtaagccgt aaaggtgata tagaaaatgg aacgtgtatc
    10021 tttataacca cgcaaaatcc cactgccaat cacctggata gagtcagaaa tctgatatac
    10081 cgccgccagc agcatcaaat gcgcagccag cgttacaacc tcgggattgt cgttgtacaa
    10141 cagggcgatt tgctctcgca gtgaaaccgt gaatatggcc gtcagggttg ccatacagac
    10201 acccaccata agcccggtcc gcgcagcggt ttgcgcatcc agcgttgagc cttgacccag
    10261 acgataacct acgcggatgg ttaccgctgc cgccagcgac atgggaagca cgaacatcag
    10321 tgaactaaag ttcagggcaa tctgatgtcc tgcgacatca acaataccga gcggagacac
    10381 taacagagcc acgacggcaa acagcgttac ttcaaagaac agtgccagcg caattggcaa
    10441 accgagttga atcagtcgtt tcataaccgc aggatcgggc ttcgcggtgc ctttttcgtt
    10501 acgaatatcg cgcatcgagc gggcgcgttt aatgtaagaa accatggcaa ggaacatgac
    10561 ccaatacacc gccgcagtag ccacgccaca accaacgcca ccgagctcag gcataccgaa
    10621 atgaccataa ataaagatat agttcaccgg aatgttcacc agcaggccga taaagcccat
    10681 caccataccc ggcttggttt ttgccagacc ttcacactgg ttacgggcaa cctggaagaa
    10741 aagatagccc ggcgcgcccc atagcaacgc acgcagataa cccacggctt tgtccgccag
    10801 agccggatcg atgttttcca tggagcggat aatgtaaccc gcattccaca gtaccagcat
    10861 aatgagaacg gaaacaaaac ctgccagcca gaaaccttgt cgcacctgat gcgcaatgcg
    10921 ctcacgtcga ccggaaccat ttaattgcgc gataaccggc gttaatgcca gcagcagtcc
    10981 gtgaccaaag aggatcgccg gaagccagat agaagttccg atagcaaccg ccgccatgtc
    11041 ggtggcacta tagccgcccg ccatcacggt atcgacaaaa cccatcgcag tttgggcgat
    11101 ttgcgcgaga atcaccggga ttgctaatgc taataacaga cgcgcttcac tgatatactt
    11161 ctgcacgtga acacctttta tttgtagtta tatgagagac taaaaagccg cctgaatggg
    11221 cagcaaaaag aagaagcagg ggaaattcag tctattgtag cgaggattta ttaattctcc
    11281 agtgaaaaaa tagccattct taccccttca ctggtaaggg cgttttccac ctgctattgt
    11341 gctgaacaga atgtcttaac tgatttcagg agttgtaagt atgtttacgg ggattgtaca
    11401 gggcaccgca aaactggtgt cgattgatga gaaaccaaat tttcgcacgc atgtggtgga
    11461 gttacccgac cacatgctgg acggactgga aaccggtgcg tccgtggcgc ataacggttg
    11521 ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt tttgacctga tgaaagaaac
    11581 gttacgcatt actaatcttg gtgatttaaa agtgggggat tgggtaaacg ttgagcgtgc
    11641 ggcgaaattc agcgatgaaa ttggcggaca cttaatgtca ggtcatatta tgaccaccgc
    11701 tgaagtggcg aaaatattaa cctcggaaaa taatcgtcag atctggttta aagtccagga
    11761 tagtcagttg atgaaatata tcctgtacaa aggatttatt ggcatcgacg gtattagcct
    11821 gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat ttaattccgg aaacactgga
    11881 acgcacgact cttgggaaga aaaaactcgg cgcacgcgtc aacattgaaa tcgatccaca
    11941 aactcaggca gtggtagata cggtagaacg tgtgctggcg gcacgagaaa atgccatgaa
    12001 tcaaccaggt acagaagcct gatctgaaat cgcccccgga gcgggggcga tagaatagcc
    12061 tttagcgagc gactcgaagg ccgttttcaa caccgcgtga gaacacgacc tgccagagct
    12121 gaatatcacg ggcgcggaaa gcacctgcac aggcattcag ataataggta aacatccgtt
    12181 taaagcgttc actgtagtta tccgcaattt ctggccatgc ggcgaggaat cgttcatacc
    12241 acgccatcaa cgtagtatcg taatcagcac cgaagttatg ccagtcttcc atcacaaagt
    12301 ggggttcgct ggactgagct atctggcgta cagagggcag gcaaccgttc ggaaaaatat
    12361 atttattaat ccagggatca acattcagat cggttttttt cgaaccgatg gtatggagca
    12421 ggaatatacc ttccggtttc aaattacgat ccaccaccgc aaaataggta tcgtaatttt
    12481 tcggtccgac gtgttcgaac atccccacag aaacaatacg atcaaactgg tcgttcaggt
    12541 cacgataatc ttgcagcaaa atggtgacat ccaggccttc acagcgttcc tgagccattt
    12601 tttgctgttc ggcagaaatg gtgacgccga ccacgcttac gtcataatta gacgccatgt
    12661 agtgcgccag tccgccccag ccacagccaa tatccagtac gcgcatccct ggttttaact
    12721 gcaatttttc acaaatcatt ttgagcttcg cctgctgggc agattccaga ttatcggcat
    12781 ctttccagta agcacaggaa tattgcatga aggggtcaag catgcggctg aacaagtcat
    12841 tacccaaatc gtaatgctct ttgccgacta tccaggcacg ttttttactc tgcagattga
    12901 agagacgagc gccggcaata cgcagcgtgt ctttgaaatg atggggaagt tggttctcga
    12961 gacctgcgcg taagactttg ctaaaaaaca tatccagtcg gtcacattcc caccagccat
    13021 ccatataact ttcgcctaac cccaacgatc cttcctgcag aacgcgttta aaaaaatcgg
    13081 ggtttttgac acgaatatcc gccggggcag aaccgttaat ggctataccg gcacggctaa
    13141 gtaattcgtt ggcgatacgg taccagttgt catctggtac actgacttct tctatacacg
    13201 atgaactcat agtttctcca tcactggtcg gtgatcagaa ccttaaaaca gcgtagacgc
    13261 tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga gtctataatc cgacgtagaa
    13321 cagagggaaa ggaggaaccc ttgccgaaat gaccatccat agtgagtcgg gaacgatcct
    13381 gtccccgtaa taacataata tttatcgtag ttttaacaac tcgaaattag tataggccgc
    13441 taatttcaat catacaacaa aattttgtta gcagcctaag tataagtata tcgatataga
    13501 tcagtgtgat tcactatgag cgacttcggc attgccatga ttctggcagc caacttcttc
    13561 acaacgttgc atcatgtaac ccagagcgac cagcactact gttgataaca tcacgctggt
    13621 ggtggtgagc aatggcgtac tgatactgat aagccaggaa actaccagac ttgcgaggaa
    13681 gcacagaccc agttgcagag tgttctgcaa cgctgcggcg cgaccggttg catgtgggaa
    13741 gggacgcagc gcctgggcaa caacaatcgg gtagatcgcg ccgttggcaa tcgccatcac
    13801 acagaatggg atcaggattt cgaccaacga cacatggcta atgaagcccg cagcccaggt
    13861 cgcaatgacg ctgacagcaa acagtaccag caaccacggt aataactgct tgccttgcca
    13921 tttctgcagc gcggcgcgac agccataacc accaatcaga aacgcgatag tttgcgggac
    13981 ataacttaaa ccaataactg ccgggctgta acccatttca ctaaggatga acggtgaacc
    14041 ggtcagccat gcaaaaaaac tggctgaaca ggctgcgtat atcagcacgt tgccgcgata
    14101 ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc tgactattgt tacgggcctt
    14161 cgtcgtgggt ttgagccaga aaataggcag aatcagcacc acggtaatgg caaacagggt
    14221 ggcgaaaatc gcctgccagg aaaaatggac cagcagccag cttcctaaca gaggagccag
    14281 tgccggagat agacccacca gcggcatgat ggtcgcaaaa atacggttaa ctttctgtga
    14341 aggataataa tctgtcacta atgcttgcca gataaccgcc gcggcgcaga cacccacagc
    14401 ctgtacaaaa cgcaatatca gcagcgtggc ggcgttttcc acccacagca tccccagact
    14461 acctaacgca aaaattgtca ggccgatgaa taacaccggt ttgcgaccat aacggtcgga
    14521 gagtggcccc cacagaagct gggctgcggc aaaaccggca aggaacagac taaggctggc
    14581 actgacagca gacgcaggcg tttgcaggtc ggcctgtatg gcggcgaaag caggcagata
    14641 catatcggtt gccagaaaac cgagtacgct caaacccgcc agccagacta aaaatctttt
    14701 cccaggttgc atgtatattc tctaaatctc tcaattaaac accgccgcaa agtgtaggga
    14761 gtgaaattag tgttgtgaaa cggtaatatt tgatgattgg tttcaaaaat tttgcaggca
    14821 gactatgtgg tcagaatatt ctctcgaagt tgttgatgcg gtagcgcgta atggtagttt
    14881 tagcgccgca gcccaggagt tgcatcgcgt tccttctgcg gtcagctata ccgtgcgtca
    14941 gctggaagag tggctggcgg tgccgctgtt tgaacgacgt caccgtgatg tggaactgac
    15001 cgcagccggg gcgtggtttc tcaaagaagg gcgctctgtt gtcaaaaaaa tgcagatcac
    15061 ccgccagcaa tgtcagcaga tagcgaacgg ctggcgcggt cagttagcta tcgcagtgga
    15121 taatattgtc aggccagaga gaacacggca gatgatcgtt gatttttatc gccattttga
    15181 tgatgtcgaa ctccttgtct ttcaggaagt gttcaacggt gtctgggatg cactttccga
    15241 cgggcgcgtg gaactggcga ttggcgcaac acgcgcgatt ccggtaggcg gtcgttatgc
    15301 cttccgggat atggggatgt taagctggag ttgcgttgtt gccagccacc acccgctggc
    15361 gttgatggat ggcccgttca gcgatgatac gttgcgcaac tggccgtcat tggtgcgcga
    15421 agacacctcg cgaacgctac ctaaacgtat tacctggttg ctggataacc aaaaaagact
    15481 tgtcgtgcct gactgggaat catcggcaac ctgtatctcg gcaggattat gcatagggat
    15541 ggtgccaaca catttcgcca aaccgtggct taatgagggg aagtgggtag cactaaaact
    15601 ggaaaacccc ttcccggatt cggcatgttg tctgacatgg cagcaaaatg atatgtcgcc
    15661 tgcgttaacc tggttgcttg agtatctcgg cgatagtgag acgctgaata aggagtggct
    15721 gcgggagccg gaagagactc ccgcaacggg tgattaacga cgatagtcgc ggaacgggcc
    15781 gtcagccacg gagcggcgtt caatcaaccg cggatgcact tcaatggact gcggttcttc
    15841 acgtttgttg acgatacgat ccaacagcat gttgaacgct gtttcgccca gcgaatcttt
    15901 tggctgatgg atcgtggtca gcgccggcgt aaaatagcgg gcgttgcgca cgttatcata
    15961 accgatcagc gaaacatcct gcggcacgcg cagacccatt tcatcagcag cacaaagtgc
    16021 gcccattgcc atgatatcgc caccacagaa gacggcagta gggcgatgcg actgcgacag
    16081 gatttgctgc atggcgcgat aaccggattc aggttcaaag tcaccctgca caatccagct
    16141 ttccggcact ttgatcatcg cttcttccat cgccttcata aaaccagcaa gacggcctgc
    16201 gccggtgtta cgttccagcg ggccagggat aacgccgatt tcgcgatgac cgcgttcaat
    16261 cagataacgc ccggccatgt agccgccttc gaacgcgtta tcaatgaccg catcggtgaa
    16321 gtcagctttt gcttcacccc agtccatcac caccattggg atatggcgat actcttccag
    16381 catcgccagc aacggctctg ggtactcaga acacatcacc agcagaccat cgacgcgttt
    16441 ttgcgccatc atcgacagat aagcccgctg tttctcaaga ttgttccacg cattgcccag
    16501 aatcagggtg taacctttct ggaagcaatt tttttcgacc gcttcaatga tctcggcaaa
    16561 ataggccgct tcgctgctgg tcgccagcaa accgatagac ttggtgtggt taaccttcag
    16621 gctacgcgcc accgcgctag gggagtagtg taattctttg attgctgccc acacggcatt
    16681 gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg tgtgacacag ttgtagtgga
    16741 aacgtttgct cgtttcgcta catcttttat tgttgccatt ccatttcact ccagacccta
    16801 acttcagctc ctgcaaattc gcaaggtaaa cgtttgcctt tacacacctt acgcacaaca
    16861 aaaatgttgc ggtacgccgg aaaaacgaca cggagcgtca ggaaggggtc aatggccagt
    16921 acgcaaatag tgtaagcgtg gaattttggc cgatcttgac gaaaagggga agtagtaaaa
    16981 acacttatca ttataaatga cgggagattt tttcatcaca gtgtgtaaaa atgcgtaata
    17041 tcactttttg tgtggtaata aaaaaggaga aaacttgatg agcaccgacc ttaaattttc
    17101 actggtaaca acgattatcg tcctcggttt gatcgtggcc gtgggtttga ctgccgcgct
    17161 gcactgattt cctctcgagg gagcgcgttc tccctcgcat ctccttcctg tttgttagca
    17221 aaatggcaaa agtgttttct ctttttgtta ttttcatttt tttgtgatta ctgtcatgct
    17281 ttcgccactt taatgaatat cgcgtaatga cgcgaagagg tggagataag gcatgaaaat
    17341 taactatccg ttgctggcgc tggcgattgg cgcgtttggt atcgggacaa cggagttctc
    17401 gccaatgggc ttgttgcccg tcattgcgcg cggtgtggat gtctcgattc ccgctgccgg
    17461 aatgttaatc agtgcttatg cagttggcgt aatggttggc gcgccgctga tgacgcttct
    17521 actttctcat cgtgcccgcc gcagtgcgtt gattttcctg atggcgattt tcacgcttgg
    17581 caacgtactt tccgccatcg cgccggatta tatgaccctg atgctttcac gcattttgac
    17641 cagcctgaat cacggggcat tttttggttt gggttcagtc gtggccgcaa gcgtagtgcc
    17701 aaaacataaa caggccagcg cagttgccac tatgtttatg gggttaaccc tggcaaatat
    17761 cggtggcgtg ccggcggcga cctggttggg cgaaaccatc ggctggcgga tgtcatttct
    17821 ggcaacagcg gggctgggcg tgatttcaat ggtaagtctg ttcctctcat tacctaaagg
    17881 tggcgcaggg gcgcgacctg aagtgaaaaa agagctggcg gtattaatgc gtccgcaggt
    17941 gctgtctgca ttgctgacga cggtactggg cgctggtgca atgtttaccc tctacaccta
    18001 tatctctccg gtactgcaaa gcattaccca cgtaacgccg gtgttcgtca cggcaatgct
    18061 ggtgctgatt ggtgtcggat tctctatcgg taactatctc ggcggcaaac tggcagatcg
    18121 ttcagttaac ggcacgttga aaggcttttt gttgttgctg atggtgatta tgctggcaat
    18181 cccgttcctg gcccgcaatg agttcggcgc agctattagc atggtggtgt ggggagctgc
    18241 aacctttgcg atcgtaccgc cgttacagat gcgcgtgatg cgtgtcgcca gtgaagcgcc
    18301 aggtctgtct tcatcagtca atattggtgc ctttaatctt ggaaatgcgc tgggagcagc
    18361 tgctggtggt gcggtaattt ccgctgggct gggatacagt tttgtgccgg tgatgggggc
    18421 gattgtcgcg ggactggcat tattgctggt gtttatgtca gccagaaaac aacctgaagc
    18481 agtttgcgtt gccaacagct aattaaacaa aaacgcagaa ggataacctt ctgcgttaca
    18541 gttgctggca acgtgcgatt tatcttgccg aatgcgctgt taacgcctca tccgcgttgg
    18601 catggtaagc gaagcgcatc aggtaatgtt gcatttgcca tcagttatta tgcagcgaga
    18661 tttttcgcta cgaattccca gttcaccagc gcccagaagt gctccagata gccagggcgt
    18721 gcattgcgat agtcgatgta ataagcgtgt tcccagacat caacggtcag cagcggagtc
    18781 gcatcggtgg tcagtggagt acccgcgtta gaggttgaaa cgatagccag tttgccatcg
    18841 ctgtttttca ccagccaggt ccagccagaa ccaaagtttt tgatcgctgc atcagtaaac
    18901 tgagctttga aatcggcaaa gctgccaaaa gatgcggcga tagcttcagc gacttttcca
    18961 gtcggttcgc cacccgcgtt cggtgccagg cagttccagt agaaagtatg gttccagacc
    19021 tgagctgcgt tgttgaatac gccaccttca gagctgcgaa taatctcttc cagtggttta
    19081 ccttcaaacg cggtaccttt gatcaggttg ttcaggttag tgacataagt ctgatgatgt
    19141 ttgccgtagt gatactcgat ggtttccgca gaaatgtgcg gtgccagagc atctttagca
    19201 tatggtagtg caggtaattc gaatgacatt gctactctcc tttattatta atttgcatac
    19261 gtacaatagc cttattgtgc gtatggatag ggtagcaaat taaaaggtga gacaaaagat
    19321 gaacttaccc tgttgccgta acaacagggt aaagttttag cgaagtgttt ttggggtcat
    19381 tacccggcga gcgccaacat agtggcgctg ccagtagtct tcactgagag aagtgatttg
    19441 aatttcctgg cccgtgcgcg gtgactgaat aaatttgccg ttgccgacat acacgccgac
    19501 atgatcggct gtaccgcgtc cctgagtacg gaagaagacc aggtcgccgt ttttcagttc
    19561 actacgttcg attggcgctg catcgcgcag gtgatacatt tcattcgccg tacgcggaat
    19621 acgaattttc accaaatctt tataagcgta ataaaccaga ccgctgcaat caaaaccggt
    19681 acgcggtgag ctgccacccc aacgatatgg cttaccaatt tgctgcatca gtttattcat
    19741 tgccactttt gtggcttttt gcaccttcgc tttgtgcgcg tcggcaaggg ttcctgacgc
    19801 ggcatttttg actttcacac aatgcgattt atagccctta cgtttggtac acttttcggt
    19861 aaagctggct gaggccgttt tattgacggc gtgtttgctg cgagttttgg cgtttttaga
    19921 agcggtaatg gaagattttt taacggaaga agcggttttg cttttggtcg tacttttact
    19981 ggcggctttt ttcgctgttt tctggctggc tttctgggtc ttttgggtgg ttgccgtgct
    20041 tttcttttta tctgcttttt tggtgatatg agtggtagca gaactctccc tggcttgctt
    20101 tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat
    20161 cgaaatacgg tttatccgcg ccactgagcg tccctctgtt aaggcaggtc tatcatgcct
    20221 gcgtctgatt tacaaaactt gatgattcta atgcatagaa gaagagaaag ttaatagctt
    20281 ttattgttaa aaaatacgtt tcgtcggcga gcgcaaaaaa atgcagcagt gaaagctaaa
    20341 aatggttata aatacgccag tttgccgtgc atcagggaag aaacgaatat ttgaaatgca
    20401 acaaatagag caaagggata aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc
    20461 gcactgaccc gctacaatgt cagattactg ccgtaaagaa gtttgaggaa gcaagacaat
    20521 gagcaccact atcgaaaaaa tccaacgcca gattgctgaa aacccgatcc tgctgtacat
    20581 gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc
    20641 cgcatgtggc gaacgttttg cctatgttga tattctgcag aatccggaca ttcgtgcgga
    20701 actgccgaaa tatgcgaact ggccgacctt cccgcaactg tgggttgacg gcgagctggt
    20761 gggcggttgt gatatcgtga tcgaaatgta tcagcgtggt gaactgcagc agctgatcaa
    20821 agaaactgcc gctaaataca agtctgaaga gccggacgcg gaataatccg tcctgcaata
    20881 ctgacggatg cgaacgggca tccgtcagtc ctttctctat ccccaatcca gcccttgtgg
    20941 cgaacttgaa aatcccgcct cacgtaacag cgtaaacatc ggcgtttgcc ggaccggtag
    21001 gtcattcact tctgttagcg taaagcgcag ccgtggttcg cgactagaca gcggtcggct
    21061 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggcga aaacacgtcc ggcaggggcg
    21121 atgaagggtt tggattattt gccattaacg ctattccccg tgaatatcat gcatgatgcg
    21181 tggaatagcc tcactataga tgcctgaatc attgcatgtc atcaggcatc gactcgatta
    21241 cacctctgcg gcggcaggta gtggccagcc tcccagacgt ttccagcggt tgacgatttc
    21301 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
    21361 gaagtccatg ccagctgtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
    21421 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagtgaggc
    21481 gcgttctgcg gcggccatca taaagctgtg atcgaaattg gcgttgtgcg ccaccataat
    21541 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
    21601 cgcctcgtac tcgctgaccg cgccgcgatc gggatcgttc ggatcaatgc cattgaaggc
    21661 aagggcctct ggttgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
    21721 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
    21781 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
    21841 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
    21901 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
    21961 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttctc
    22021 ttcgattaat tcaattttgt aaccgtccgg atcttccaca aatgcgataa ccgtagtacc
    22081 gccttttacc ggacccgctt cacgggttac gttacccccg ttttgacgga ttttttcgca
    22141 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
    22201 tttatccacg ccccagttgt aggtcagttc aatcactgct tcttcggttt ccgggccgta
    22261 accaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
    22321 gcccagcacg ttggtataaa aatcgatgga gcgttgcaga tcgccaacgc gcagcatggt
    22381 atgaagaaga cgcatcttta tatcctcaaa atggctaaca gattcaaaaa gttgttttag
    22441 tatagcggcg gctttgcgcc gctcgcaatg ttggattaca gcgtcgggta atcagtgtaa
    22501 ccttcagcac cgccaccata aaaactttcg gcacgctgcg ggttaagctc agctttgcgc
    22561 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc taccgcatca
    22621 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
    22681 atcggaccgt ggaaacgggc gcgtactttt tcgcgaaacg catcagtata tggttcacct
    22741 cccgcccaat ctggttctga catatgaaga taagcaatgc cgcgtttacc cagttgttca
    22801 atcagataca gtgcatcggc ttcttcattc gggccgttat ccgtgttctg gaaagtaccg
    22861 attggcgaaa tgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
    22921 acttccagca ccagacgcgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
    22981 tttgaagaag gggaaaggaa ctgatgcagc aaataaccgt gagcagagtg gagctctacc
    23041 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgataccc
    23101 ggaatctctt ccagttcaag cgcacgcggc atggatgttt caacacggat cgcctgacca
    23161 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
    23221 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgtacggcc
    23281 atatgaccat tttcagcatg aacgccagcg gtaatttttt tccatgcggc aatttgttcc
    23341 gggctatgga tgccaggtgc acctgcgtat ccttttgcct gggcagaaat ttgcgtggct
    23401 tcgctaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
    23461 gggatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
    23521 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
    23581 cccggtcgtc tggtgataaa aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
    23641 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcaaattc
    23701 gcaccaagcc acagtgcgta aagcacttgt gcctgttgca gcggttcgcc acaaaaggtt
    23761 aaacaatggt tctcacggcc attttccagc gcctgagaga gcagggcgat cacgccgcga
    23821 gcccctttat ccattgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
    23881 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
    23941 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
    24001 cgctggtgat atgcggcgta atggcgctca agcatggcaa cgccaaacgc ttctttagaa
    24061 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttaggag ttcgcttagc
    24121 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg
    24181 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
    24241 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
    24301 aggagggcga ggtgacggag caattagagt tctttcccgt ccagagcccg tgccggggaa
    24361 tttgccagtc tgacgaacgg ggattttgtc gcggctgttt tcgtagccgg gatgagcgtt
    24421 ttaactggaa taaaatgagc gatggcgaaa aacaagaagt gttgcgcctt tgtcggcagc
    24481 gattaatgcg caaattacgt gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
    24541 cactcttttg agcctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
    24601 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccatttt gtgatgggct
    24661 actggcgatt gatggactgg aacatgtccg cccgccagtt ggtcagtttt attgaagagc
    24721 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
    24781 aagcggcgtt tggcgaggcg ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
    24841 tcagtaaatg cggtatcgcg acgaccgcgt gtgaagaaaa cgtcattggc cattacatca
    24901 ctgaccgcga tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
    24961 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
    25021 acgcctttaa acatctgcat cagagcggca aagtgcgtca ttttggtgta tcgaacttta
    25081 cacctgcgca atttgcgctg ttgcaatcac gcctgccatt tacccttgcc actaatcagg
    25141 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactgcaac
    25201 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
    25261 attatttcca gccgctgcgt gatgaactgg ctgtggtggc agaggagtta aacgcgggct
    25321 cgattgaaca ggtggtttac gcctgggtat tacgtttacc gtcgcagccg ctgccaatta
    25381 tcggctcagg taaaattgag cgcgtacggg cagcagtcga agcagaaaca ctgaaaatga
    25441 cccgtcaaca atggttccgt atccgtaaag cggcactagg ttacgacgta ccgtaagcac
    25501 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
    25561 aatgaacgga ggtcctatga aacgttttag tctggccatt ctggcgctgg ttgttgcgac
    25621 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggttgg
    25681 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcgcccga
    25741 tctgaaagca ttacctcccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
    25801 gccagccacc aaagatggca aagccagcgc cgcagaatcc gcaggcgggc atcttgatcc
    25861 acaaaatacc ggtaaacatg aagggccaga aggtgccggg catttaggcg atctgcctgc
    25921 actggtagtc aataatgacg gcaaggcgac cgatgccgtc atcgcgcctc gtctgaaatc
    25981 actggatgaa gtcaaagaca aagcgctgat agtccacgtt ggcggcgata atatgtccga
    26041 tcaacccaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    26101 aagaggccag cgtaccttgc ggcggtgctt gctcaagttg cgaaagcgag cagtacagcc
    26161 gccagacaat tgccgccagt tctcgggcgg caggttgatg atgacgggca aggctgtcgc
    26221 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    26281 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    26341 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcaac actacacccc
    26401 agcgtaataa ccaacggcga gccagcgcat cctggctgtt actcaactga ctgacgtgat
    26461 gataagtgag cgattcaaat tcgctttcac tcagtgttgg atggcggctt agttgatcga
    26521 caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt acgagcatcc gatcctggac
    26581 gcagaatggc gaacgctaac cacgccaacg cgacgccaac aatttttgcc agattatcgt
    26641 taagaaaatc agcaaaatca tacaccgggg gattagtgac agcgataaac gaacccataa
    26701 aaacaatcag ttgcccccac agtgcggcaa atttgggcat ctgcaattta agaagctgca
    26761 ttgtcgccag cagtggaaag agaaacagta aaaattgcca cagatcgcta atctgcacca
    26821 tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa caccagcgtg cgcatcagca
    26881 gtgacaaaga cttaaacggt gctgcgacgg cggagtagag tacgcagcta attgctgcca
    26941 gcgttaaagc attggcaccg gcatcccatt gcgaagcaat actccatgcg ccaatcatca
    27001 tcaacgtaca aaatgtacgc agcccgctcc acatagcttc ggcgttatcg gtatgacgag
    27061 ccaggccgga tgtccgtggg agtctggcat gatcatctac accgctttgt agacgatgta
    27121 attcctgact actttgcaga taaaggcggc aaaaataacg tagtcgctgc cagaaggcga
    27181 tgtgccggta gtcggtgacg ttggtcgggc gtagtgggga gataatacgt gcgacggtgt
    27241 aaacatctgt ttgcgaactg gcgagcgccg tcagcagctg ttcgagaatt tctcgcgtgg
    27301 catctggcgg tgagggccag ttgagcaaca tacgtcgcag gctggagatg acactggtca
    27361 tacgtaattg ctggtggagc agcgcattaa ggcgtgcgtt ttgctggcga aaacgatagt
    27421 ggctccagaa agcctggata cgcagcaaat tcatggtcag tatctgccca atcacccctt
    27481 catgtgctgc acgaatggca tcggttgttt caggccgcca gagtaaactg gcatgttcca
    27541 gtaatcgggc gtgcatgttt ttcaatgcgg ttaaaagggc tgtagcatcg gaactgctcg
    27601 gcaggatcat catcatcatg ccgccgcaca aaataccgac tatcacctcg caaacacgcg
    27661 cctgagcgat atcccacagc tggctggcct cagtaatatt aaccatcgga aaggcgatga
    27721 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
    27781 aggcccaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
    27841 tgagcgtatg tccggcaaga agcagtgctg caatggctcc gagcaaactg ccagcgatgc
    27901 gtccgaggct tttgctgatg acaccgccaa cggtgggaaa gctaaccacc gcagccgagg
    27961 tcatcgccca atagggttca tccagattca aataataggc aaccgtcagc gccagacaca
    28021 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagggca
    28081 aattgcgcaa ggaccatgac gatgcgttca ttattgacct acggcaatgc tacaggtggt
    28141 gcccgacacc agcgtgacat cgcctgggac tttatccagc gtaaagcgga cgggaacgcg
    28201 ttgggcgagg cgaacccagg ggacgttagg tttcacatcc ggaataaggc ttgagtcact
    28261 ttcaacgctc tgatcataaa tcgcccgacc gatactggac acgtgaccct gtaacgtttt
    28321 gttatcacta tagagggtaa tttgtgctgg tgcaccttcg cggatatggc gcaacttggt
    28381 ttcttcaaaa taaccaatga cgtaaaacga gtggctgtca acaagggcaa acagcggttt
    28441 cccggtgtca gcgtagtcgc cgatgcgggt agtgagatta gtcacccatc cactcaccgg
    28501 ggcgcgaatt tccgtttgcg ccagttgcca ttgtgcctgt ttcagcgtcg cctgtgcggc
    28561 atcaacgctg gcctgcatcg ctttaacatt gaggttagcg gtatcgagtt cttcggcaga
    28621 aataaaattt tgtgagagat gacgacgacg attcgcttcg ttattggctt ttgcgaggtc
    28681 agattgcgct ttcgccagct gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt
    28741 atcgatcgtg agcagaagat cccccgcgtt aaccagctgg ttgtctttta tattcagctc
    28801 aacaatacgt cccgacacct gtggggtgat agaaacctgt tctgcgcgta ttttcccgtc
    28861 gcgtgtccac ggagattgca tgtaataatt ccacagccac catccggcaa gaaccgcaac
    28921 taccgctaca atgattgttg agaaatactt aattgtttta attgacatag ttaccacgca
    28981 atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag
    29041 ggatgccaga tgtcaccagc gtagatccag ccacgaagca ggcggtgtat gacaagccag
    29101 atgacgaatc caaacgcgaa ggctttgaaa aacggaggaa agtagacgga cgcaccgaac
    29161 accagatctt gtaagggcaa tcctgttgca ttgagcataa acttcacaaa gtagattcct
    29221 ttacgacaga atatattgcg ttagattaat aaatattctt taagtgcgaa aaatttacgc
    29281 gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag caatacattt gttttagcaa
    29341 tacaattgct gcacactatt ctaaaagccg cataatatct tagcaagcta attataagga
    29401 gatgaaattg gaatcgccac taggttctga tctggcacgg ttggtgcgca tatggcgtgc
    29461 tctgatagac catcgcctga aaccgctgga gttaacacaa acccattggg ttacgttaca
    29521 caatatccat cagttacctc cagaccagtc gcaaattcaa ctggcaaaag cgattggcat
    29581 cgagcagcca tcactggtcc gtacgctgga ccaactggaa gaaaaagggt taatttcgcg
    29641 tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc
    29701 actgatcagc gaaatggagg ctgttattaa caaaacccgc gcggaaattt tacatggcat
    29761 ctccgcagag gaactggaac aactgattaa gctcatcgca aaacttgagc ataatatcat
    29821 tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc
    29881 aaacttattt agcgcgggga aacggtcacc tgactgccat tgctggccag tacgacacgt
    29941 tggcctggag agaaatgagt gttcccttgt ttctgtacca ccatgatggt gttgccatca
    30001 tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg tacgccctga
    30061 ccggctacac cacctgcaac ggcccctgct gcagtagcca gagaacgccc ggttccgcca
    30121 ccaatagtat ttcccaggaa accaccaagt acagcaccgc caattgcgcc gataacgttg
    30181 gaatcatcac cgccctgaat ctgtacctga cgtacattaa cgatggtgcc atagctgaca
    30241 ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt gtcgttatta
    30301 acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt aatcattgaa
    30361 aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa gtatatggca
    30421 tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg aaagtaggat
    30481 caaccaatta taaacaaaat attcatgatg attattgatt tgaagataat gttttactac
    30541 caacggtaaa aattggtcat tccgcgtggt attcacgaaa agtttatgtt tgagtggtgc
    30601 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
    30661 caggcaccag ccttgatggt gttgatgttg tgttagcgac aattgatgaa caccgggtcg
    30721 cacagctggc aagtttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
    30781 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
    30841 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
    30901 tcgttgcgat cggctgtcac ggtcaaaccg tctggcacga accgacgggc gtggcaccac
    30961 acactttaca gattggcgat aacaatcaaa ttgtggcgcg caccggaatt acggttgtcg
    31021 gtgattttcg ccgtcgcgat attgccctgg gaggacaagg tgcaccgctg gtacctgcgt
    31081 tccatcatgc gctgctggct cacccaaccg agcgacgaat ggtgctcaat attggcggca
    31141 tcgccaatct gtcactgctc attcctggcc agccggttgg gggctacgat accggtcctg
    31201 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
    31261 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
    31321 catatttctc gcaacccgca ccgaaaagca ccggacgcga atactttaac tacggctggc
    31381 tggagcgcca tttgcgccat tttccggggg ttgatccccg tgatgtgcag gccacagtgg
    31441 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
    31501 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
    31561 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
    31621 cattggcgtt cgcctggctt gcctggcgaa cgctggcggg attaccagga aatctgcctt
    31681 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccccgct aacccgtgat
    31741 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
    31801 gcgtattgcc ctccagacca ggaaagtctt cgggatatgt ctatgaaaaa actgttactg
    31861 atctgtttgc cggtgcttct caccggctgt agcgcgttca atcaactggt tgagcgcatg
    31921 caaaccgata cgcttgaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
    31981 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
    32041 tcagcgtctg gtgcacgtta caccgacgga atctatgttt tctggtcgaa aggcgaggaa
    32101 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccgcag
    32161 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
    32221 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
    32281 aggcgggtta cgccgccgcg atctacccgc cgatccatta accctttttg aacgttggct
    32341 ctctcaggct tgtgaagcca aactggctga ccctaccgcg atggtggtcg ctaccgtgga
    32401 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
    32461 ggtgttttac accaacctcg gcagccgtaa agcacatcaa atcgaaaata atccgcgcgt
    32521 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcgga
    32581 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
    32641 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
    32701 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccaa gtttttgggg
    32761 tggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
    32821 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
    32881 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
    32941 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
    33001 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
    33061 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
    33121 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
    33181 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
    33241 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacactga agaaactgtt
    33301 caggagtggg tggacaaaat ccgcaagcag gttgccccgt tcctcgattt cgactgtgga
    33361 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
    33421 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
    33481 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
    33541 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaatcggt
    33601 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
    33661 aatcaggtgt ttggcttgac cgttccgctg atcaccaaag cagacggcac caaatttggt
    33721 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
    33781 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttc
    33841 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
    33901 cgcgcccagt atgtgcttgc agaacaggtg acacgtctgg tgcatggtga agaaggtttg
    33961 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ccttgagtgc gctgagtgaa
    34021 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
    34081 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
    34141 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
    34201 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
    34261 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
    34321 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
    34381 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
    34441 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggaccggttg
    34501 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
    34561 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
    34621 cctcggtatc gttcgtcagg tgaaagcagc gaatccacag gcgaaatatt tttgcgatcc
    34681 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
    34741 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
    34801 ctgtgagcat ccggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
    34861 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
    34921 ttttgaaatg ctgctagtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
    34981 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
    35041 actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg cagtatacga
    35101 aatcatggtg accacaaaag caatgcagga atatgagctg caagtggttg ctgctcagga
    35161 tcgtattgcc aatccagaac attatttcag cgcaacaaag ctctgaaata tctcatttcg
    35221 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
    35281 tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc taacccttcc
    35341 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
    35401 gtgaatcgtt gcccgcagat ccagtgctca tccttcaggg cctcgttcac atattgcagc
    35461 ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc agggcgaaac
    35521 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
    35581 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
    35641 tactgcataa tcgctacgcc ttccgtcagc aaagtaccat cgtccagcag caatgcaggt
    35701 acctgtccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
    35761 aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
    35821 agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
    35881 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
    35941 taaatgagtt tccctgaatt ttacgctacg gctgctttcg ccgctttgtc tgcagcgtca
    36001 tcctgcgtca tgcggtgcag tttcggcgcg gtaaacagca tcagtactgc aataacggca
    36061 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcggatcg
    36121 gtaacgttat ccggcacagc catcatacct gcaacataac caccaatcag gtttgcaccg
    36181 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
    36241 gcaaccattg ccagacccag gccagagatc atcagttccc cgatactctg caggccatag
    36301 ctcgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
    36361 ggcagaatca ggaacgcacc agaacacatc accatgccga tagcaaactt ggttggcatc
    36421 ggcagggtat cgcccatctt gttatagata gcggccagaa tcgggctacc aatgatgatc
    36481 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccaggcccag aatggtgtgt
    36541 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctata aagcacgaag
    36601 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
    36661 ttcatcgcga aggcttcttt accgaagata accacaatac cgaaggcaac aacgcccagc
    36721 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
    36781 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
    36841 ccgtactgtt taacccaacg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
    36901 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
    36961 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
    37021 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
    37081 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
    37141 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
    37201 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
    37261 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
    37321 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
    37381 gagaagatga gatagaacgc cttcggttgt ttaaaagcgt tcaaactgac gctttcagtt
    37441 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
    37501 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
    37561 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
    37621 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
    37681 aacgtaacgg cagatttttt cactaccctg agagggtgat gatggcttaa cggcgatatg
    37741 ttctactatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat agccagtgta
    37801 atgttttgta ggtcaatatt tgttgtgtgt gtaaatttaa atgattttaa cctgattctt
    37861 gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
    37921 ctattaaaaa aacaattaac ctgcattttt ggttatctgc aaacggatta cctgatgata
    37981 acgctaagca atggcatcat tgacgttaaa a
//
LOCUS       DAPQZD010000030        37649 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPQZD010000030 DAPQZD010000000
VERSION     DAPQZD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.030"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            31..753
                     /gene="mngR"
                     /locus_tag="RO454_001470"
     CDS             31..753
                     /gene="mngR"
                     /locus_tag="RO454_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDY0933318.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
     gene            complement(857..1726)
                     /gene="sucD"
                     /locus_tag="RO454_001471"
     CDS             complement(857..1726)
                     /gene="sucD"
                     /locus_tag="RO454_001471"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDY0933319.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(1726..2892)
                     /gene="sucC"
                     /locus_tag="RO454_001472"
     CDS             complement(1726..2892)
                     /gene="sucC"
                     /locus_tag="RO454_001472"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDY0933320.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(2986..4203)
                     /gene="odhB"
                     /locus_tag="RO454_001473"
     CDS             complement(2986..4203)
                     /gene="odhB"
                     /locus_tag="RO454_001473"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDY0933321.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4218..7019)
                     /gene="sucA"
                     /locus_tag="RO454_001474"
     CDS             complement(4218..7019)
                     /gene="sucA"
                     /locus_tag="RO454_001474"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDY0933322.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(7381..8097)
                     /gene="sdhB"
                     /locus_tag="RO454_001475"
     CDS             complement(7381..8097)
                     /gene="sdhB"
                     /locus_tag="RO454_001475"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDY0933323.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8113..9879)
                     /gene="sdhA"
                     /locus_tag="RO454_001476"
     CDS             complement(8113..9879)
                     /gene="sdhA"
                     /locus_tag="RO454_001476"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDY0933324.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(9879..10226)
                     /gene="sdhD"
                     /locus_tag="RO454_001477"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(9879..10226)
                     /gene="sdhD"
                     /locus_tag="RO454_001477"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDY0933325.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10220..10624)
                     /gene="sdhC"
                     /locus_tag="RO454_001478"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10220..10624)
                     /gene="sdhC"
                     /locus_tag="RO454_001478"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDY0933326.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11005..11112
                     /locus_tag="RO454_001479"
     CDS             11005..11112
                     /locus_tag="RO454_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933327.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11318..12601
                     /gene="gltA"
                     /locus_tag="RO454_001480"
     CDS             11318..12601
                     /gene="gltA"
                     /locus_tag="RO454_001480"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDY0933328.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSDIKK"
     gene            complement(12648..13958)
                     /locus_tag="RO454_001481"
     CDS             complement(12648..13958)
                     /locus_tag="RO454_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDY0933329.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
     gene            complement(13958..14419)
                     /locus_tag="RO454_001482"
     CDS             complement(13958..14419)
                     /locus_tag="RO454_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDY0933330.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            complement(14479..15447)
                     /locus_tag="RO454_001483"
     CDS             complement(14479..15447)
                     /locus_tag="RO454_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HDY0933331.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            complement(15475..16671)
                     /locus_tag="RO454_001484"
     CDS             complement(15475..16671)
                     /locus_tag="RO454_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDY0933332.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            complement(16671..16982)
                     /locus_tag="RO454_001485"
     CDS             complement(16671..16982)
                     /locus_tag="RO454_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDY0933333.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            complement(16997..17752)
                     /locus_tag="RO454_001486"
     CDS             complement(16997..17752)
                     /locus_tag="RO454_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0933334.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            complement(17764..18777)
                     /locus_tag="RO454_001487"
     CDS             complement(17764..18777)
                     /locus_tag="RO454_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDY0933335.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            complement(18790..19476)
                     /locus_tag="RO454_001488"
     CDS             complement(18790..19476)
                     /locus_tag="RO454_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HDY0933336.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            19740..20453
                     /locus_tag="RO454_001489"
     CDS             19740..20453
                     /locus_tag="RO454_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDY0933337.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            20633..21679
                     /locus_tag="RO454_001490"
     CDS             20633..21679
                     /locus_tag="RO454_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDY0933338.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIIAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(21676..22467)
                     /gene="nei"
                     /locus_tag="RO454_001491"
     CDS             complement(21676..22467)
                     /gene="nei"
                     /locus_tag="RO454_001491"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDY0933339.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(22503..23237)
                     /gene="pxpA"
                     /locus_tag="RO454_001492"
     CDS             complement(22503..23237)
                     /gene="pxpA"
                     /locus_tag="RO454_001492"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDY0933340.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(23227..24159)
                     /gene="pxpC"
                     /locus_tag="RO454_001493"
     CDS             complement(23227..24159)
                     /gene="pxpC"
                     /locus_tag="RO454_001493"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDY0933341.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(24153..24809)
                     /gene="pxpB"
                     /locus_tag="RO454_001494"
     CDS             complement(24153..24809)
                     /gene="pxpB"
                     /locus_tag="RO454_001494"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDY0933342.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(24832..25575)
                     /gene="ybgI"
                     /locus_tag="RO454_001495"
     CDS             complement(24832..25575)
                     /gene="ybgI"
                     /locus_tag="RO454_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDY0933343.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            25846..27327
                     /gene="dtpD"
                     /locus_tag="RO454_001496"
     CDS             25846..27327
                     /gene="dtpD"
                     /locus_tag="RO454_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDY0933344.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(27369..28787)
                     /gene="phrB"
                     /locus_tag="RO454_001497"
     CDS             complement(27369..28787)
                     /gene="phrB"
                     /locus_tag="RO454_001497"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDY0933345.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(28784..29293)
                     /locus_tag="RO454_001498"
     CDS             complement(28784..29293)
                     /locus_tag="RO454_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDY0933346.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(29394..29600)
                     /locus_tag="RO454_001499"
     CDS             complement(29394..29600)
                     /locus_tag="RO454_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDY0933347.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            29913..30002
                     /gene="kdpF"
                     /locus_tag="RO454_001500"
     CDS             29913..30002
                     /gene="kdpF"
                     /locus_tag="RO454_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDY0933348.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            30002..31675
                     /gene="kdpA"
                     /locus_tag="RO454_001501"
     CDS             30002..31675
                     /gene="kdpA"
                     /locus_tag="RO454_001501"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDY0933349.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            31698..33746
                     /gene="kdpB"
                     /locus_tag="RO454_001502"
     CDS             31698..33746
                     /gene="kdpB"
                     /locus_tag="RO454_001502"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDY0933350.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            33755..34327
                     /gene="kdpC"
                     /locus_tag="RO454_001503"
     CDS             33755..34327
                     /gene="kdpC"
                     /locus_tag="RO454_001503"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDY0933351.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            34320..37004
                     /gene="kdpD"
                     /locus_tag="RO454_001504"
     CDS             34320..37004
                     /gene="kdpD"
                     /locus_tag="RO454_001504"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDY0933352.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            37001..>37649
                     /gene="kdpE"
                     /locus_tag="RO454_001505"
     CDS             37001..>37649
                     /gene="kdpE"
                     /locus_tag="RO454_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDY0933353.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITE"
BASE COUNT         8775 a        10225 c         9570 g         9079 t
ORIGIN      
        1 ggctaaattc gtattaatga gatactagaa atgggacaca aacccttata ccggcagatt
       61 gccgatcgca ttcgtgagca gattacgtgc ggcgagctaa aacctggcga tgcgttaccc
      121 actgaatcgg cattacaaac agagtttggc gtcagccggg ttacggtgcg ccaggctttg
      181 cgccagttag tcgagcagca gatcctcgaa agcatccagg gcagcgggac ttacgtcaaa
      241 gaagagcggg tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac
      301 cgtcacgtcg aaacacatag tgaagtcctg atatttgaag taattccggc tgacaatttt
      361 cttcagcagc agctgcaaat tagcgagcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc
      421 tatcgcaagc aaaagccggt ggcgctggaa gaaacctgga tgccgcttgc attattcccg
      481 gatctcacct ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag
      541 atggttatcg atcgtagcga acaggagata attcccctga tgcccacgga agagatgagc
      601 cgtctgctta atatcagcca gactaagccg attcttgaaa aagtatcgcg tggatattta
      661 gttgatggcc aggtgtttga atatagccgc aacgctttta atactgatga ctataaattt
      721 accctgatag cgcagcgaaa atcgccgcga taagcataaa aaaggccatc ataagatggc
      781 caaccatgtc gaaaccgaca gcccttcgcc cttcctgtag ggggaagcgg cgagggctat
      841 ttcttattac agatatttat ttcagaacag ttttcagtgc ttcaccgata tcagccaggc
      901 tgcgaacggt tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtacctt
      961 taccaccggc aatgatggca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac
     1021 cagcgatgta acccacaact ggcttggtga cgtgctcttt gatgtacgca gccgcttctt
     1081 cttcagcgct accgccgatc tcaccgatca tcacgatcgc ttcagtctgc ggatcttttt
     1141 cgaacatttc gagaatatcg ataaagttag agccagggat cgggtcgccg ccgataccaa
     1201 cacaggtcga ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagcg
     1261 taccggaacg ggaaacgata cccactttac ctggtttgtg aatgtgaccc ggctggatac
     1321 cgattttgca ttcccccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc
     1381 ctgcttcatc cagtttcact ttcaccgtca gcatatccag cgtcgggatg ccttcagtga
     1441 tggtgataat cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg
     1501 gtgctggtac gtagataaca gaggcggtag cgccagtggc agcaacagct tcacgcacgg
     1561 tgttaaacac cggcaggccg aggtgggtag taccgccttt acctggggtt acgccgccaa
     1621 ccattttagt gccgtatgca atggcctgtt cagagtggaa agtcccctgg ctaccagtaa
     1681 agccctggca gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac
     1741 tgcggcaaca acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc
     1801 gctgtcagcc agtttcttcg caccgagttc ggcgttgtta ccttccagac gtaccacgac
     1861 cggtacgtta acacccactt ctgctaccgc gccgatgata ccgtcagcga tcaggtcgca
     1921 acgaacgata ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat
     1981 gattttgaac gcttcggtta cacgttcttt ggttgcgcca ccgccaacgt ccaggaagtt
     2041 agccggttcg ccgccgtgca gtttaacgat gtccatcgta cccatcgcca gacctgcgcc
     2101 gttaaccata caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc
     2161 ctgtgcttca cgcggatctt cctgcgactg gtcacgcatt tcacgcagat caggctggcg
     2221 gaacagtgcg ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt
     2281 ggtaataacc agcgggttga tttcgataag cgcaaggtcg cgctccagga aaatggtcgc
     2341 caggcccatg aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttaaa
     2401 cgccagctcg cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttgtg
     2461 gatcaggtgc ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc
     2521 cataaagacc acgcgacggg aactacggtc aacaacggca ccgagataca gctctttagc
     2581 gatatcggtc gctgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg
     2641 atacgttacc agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct
     2701 gtttacaact ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac
     2761 tacccacgga ccggcaccga tttttgaagc ggcttcttct gcttcgcgtg gagtagtaca
     2821 ggcataaccc accggtgctg gtaagccata gcgggcaaaa agttgttttg cctgatattc
     2881 atgtaagttc atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg
     2941 accccctatc cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag
     3001 cagacgcgtc ggatcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg
     3061 accatcgatc aaacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac
     3121 ctggccattc accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg
     3181 cggcgggttg atgataggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt
     3241 aaagttacca ccagtcagat cttcaaccgt cagcttgccg tcacggcctt taactgccag
     3301 ctctttaatt ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg
     3361 cgtcaccagg ccgcgcggcg tagaaaccgc catactgacg tcgaaatagt tgtggtagac
     3421 cacatcatcg ccgtcgatag acgcattcac ttccgggtaa cgtttcaggg cttcaaccac
     3481 cgctttcacg tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc
     3541 accgtactgc ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag
     3601 catggcggtg gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt
     3661 catcggcaca cgtttttcgc tacgtgcagc cagggccggt tgcgccgccg gagcagccgc
     3721 tgccggagca gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgggt
     3781 cagacgacca cccacacccg tgcctttaat ggcgctggcg tcgaggttat gttcagccag
     3841 caggcgacgg atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg
     3901 gcgttgcgcc ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct
     3961 gttgccttca cgcaggcgac caaggatctg acgagacgtt acggttgtac cttcatcttc
     4021 cagaaccgca tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt
     4081 ttcgatttct accagcactt catcacgtac gactacgtcg ccgggttttt tatgccaggt
     4141 tgcgacggtg gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact
     4201 cattgtgtat cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt
     4261 ttctggtgaa cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa
     4321 cgcagagaag ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg
     4381 ccctggttga gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc
     4441 aacacttcct gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg
     4501 acatcgtgtt gattgttctt acgacgttgt tccagcagat cgtaataaac cttaccagaa
     4561 cacatcacta cgcgcttcac gcccttcgga tcaagctcgt cgatttcact gatggctggc
     4621 aggaaggtgc cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg
     4681 gatttcggcg acatcacgac cagtggacga cgcatcccgc gcaacgcctg acgacgcagc
     4741 atgtggtaaa cctgtgccgg ggtagaaggt acgcaaacct gcatgttttg ctcagcgcaa
     4801 agttgcagat aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg
     4861 tgcggcagca acatcaccag gccacacatc cggccccatt tctgttcgcc agaggagatg
     4921 aactggtcga taaccacctg tgcaccgttg gcgaagtcac cgaactgtgc ttcccagatg
     4981 gtcagggtgc gtggttctgc ggtggcataa ccgtattcaa acgccagtac ggcttcttca
     5041 gacagtacag agtcccagac gcggaacgcg ccctggccgt tatggatatg ttgcagcggc
     5101 gtgtaagtgg aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg
     5161 cgaccggagt cttcacccga caggcgaacc ggaatgcctt cgtcaaccaa cgtggcgtaa
     5221 gcgaggtttt ccgcgccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga
     5281 tcgccataaa ttttggcaac gcgagactgc atttcaaccg cttccggcac cgtgctgatg
     5341 cgtttagcca gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat
     5401 tcgtggttga ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgct
     5461 acaacgcaat cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct
     5521 tccagcgtcg cgactttttc ctgctccagc ttgtcagcgt agattttgcg cggcgtcgga
     5581 tgttttttga ttttctgata catcagcggc tgggttgcgc tcggctcgtc ggcttcgttg
     5641 tggccgtgac ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg
     5701 aaatcgagcg ccagacgggt cacaaaggca acggcttccg gatcatccgc attaacgtgg
     5761 aaaatcggtg cctgaaccat cttaccgata tcagtacagt acggcgtcga acgggcatcc
     5821 agcgggttag aggtggtgaa gccaacctgg ttgttgataa cgatacgtac cgtaccgcca
     5881 acttcataac cacgcgcttt cgacatgttc agggtttcct gaactacgcc ctgcccggtc
     5941 actgcggcgt caccatgaat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt
     6001 ctgtccagac gggcacgaac agaaccgata acgaccgggc ttacaatctc aaggtgagac
     6061 gggttaaacg ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag
     6121 cccatgtggt atttcacgtc gcccgtgccg aggtgttctt tatgtttacc ggcaaactcg
     6181 tcgaacaagt cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg
     6241 tgcgccatcc cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct
     6301 ttaagcatcg ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat
     6361 tttgcgccaa ggtaacgttc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt
     6421 ttctcttcgc tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt
     6481 tcttcggtgc tggtaatgtg catatactcg gcaccaatcg ggccacagta ggtttgcttg
     6541 agggcttcca gcagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg
     6601 ttgaaggtct cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc
     6661 actttatctt gctgccacag tcccagcgga tcgagattcg catgctggtg accacggaag
     6721 cggtatgcgt taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag
     6781 atcgttgaag agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga
     6841 gagtggaatt gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag
     6901 ttagcgtcaa ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc
     6961 tggtttgcgc cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc
     7021 gtgatccctt aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacatttc
     7081 atatctgatg tgcgtgccgg ggttcacttg ttgcggactt gtcgtgtttt ctgcgtccgc
     7141 taaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt tcctgcattt
     7201 cttttgtagg ccggataagg cattcacgcc gcatccggcg ctgtttgcct gatgcgacgc
     7261 ttgcgcgtct tatcaggcct acggtttctg catttctttt gtaggccgga taaggcgttc
     7321 acgccgcatc cggcactggt tgcctgatgc gacgcttgtg cgtcttatca ggcctacggt
     7381 ttacgcatta cgttgcaaca acatcgactt gatatggccg atggcgcgcg tcgggttcag
     7441 ccccttcgga catacactga cgcagttcat gatgctgtga cagcggaata cactgaatgc
     7501 atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca cggctatcga tcaggaaacg
     7561 atatgccgct aacaagcctg ccggaccgat aaacttatcg ggattccacc agaaagacgg
     7621 acaagaggtt gaacaacatg cgcagagaat acattcatac aacccgtcga gtttttcgcg
     7681 ctgctctggc atctgtaaat gctcgcgagc tggcggattt tgtccattat tcaacaggta
     7741 aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg tctaccacca aatcgcggat
     7801 caccggtaaa cctggcagcg gacgaatcac aatcttcttg cccggctggt tgagtgccga
     7861 aatcggggta atacaggcca gaccattctt accgttcatg ttcagaccgt cggaaccgca
     7921 cacaccttca cggcaggagc ggcggaacga caggctggga tctttctctt tcagctgaat
     7981 aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct tccagggtgt aatcctgcat
     8041 acgcggagca tcatcaacat ccgggttata gcgataaatt gaaaactcga gtctcatttt
     8101 cctgtctccg cattagtaag tacgaatctt cggcgggaat gccgggcgca gtttcggttc
     8161 catgttgacg cttcggcgcg tcatggattc cgactctggc agatacaggg agtggcacag
     8221 ccagttttca tcatcacgat ccgggaagtc gaagcggcta tgcgcgccac ggctttcggt
     8281 acggaagttg gcagaaacag ccgttgcata cgccgtttcc atcaggttat ccagttccag
     8341 gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca tccagacggg catttttcag
     8401 acgctcgcgg ataactttca actgctcaag ccctttcgcc atcgcatcac cttcacggaa
     8461 gaccgagaag ttatgctgca tacattcttg cagtgcttta cggatcgcca ccggatcttc
     8521 accgttacgg ttattgttcc agcggttcag gcgatccaga gacgcttcta catcagactc
     8581 gctggcatcg cgcagtgcgc cctgctcggc gatagactct tgcagatgca gacctgccgc
     8641 acgaccaaat accaccaggt ccagcagcga gttgccgccc agacggttag cgccgtgtac
     8701 cgatacacaa gcgatttcac caacggcaaa cagccccgga acaaccacat cttcgccttt
     8761 ctcattcacc gtcagcgcct gaccggtcac tttggtcgga ataccgccca tcatgtagtg
     8821 acaggttggg ataaccggaa tcggctcttt caccggatcg acgtgagcga aggtacggga
     8881 gagttccaga atacccggca gacgggattc gagaacttct ttccccagat ggtcaagttt
     8941 cagttttgcg tgtggccccc acggaccatc acagccgcga ccttcacgga tttcgatcat
     9001 gatggaacgc gccaccacgt cacggcccgc caggtctttg gcgttcggcg cataacgctc
     9061 cataaagcgt tcgccatgtt tgttcagcag ataaccgcct tcaccacggc acccttcggt
     9121 gaccagtacg cccgcaccgg caataccggt cgggtggaac tgccacattt ccatatcctg
     9181 caccggtacg cctgcacgga tagccatgcc gacaccgtcg ccagtgttaa tgtgggcgtt
     9241 ggtggtggac tgataaatac gccctgcccc gccagtcgcc agcactgtcg cgcgagcttt
     9301 aaagtaaacc acttcaccag tttcgatgca cagtgcggta caaccgacca ctgcgccatc
     9361 ctggtttttc accagatcca gcgcatacca ctcggagaaa atggtggtgt ggtttttcag
     9421 gttctgctga taaagcgtgt gcaacagtgc gtgaccggta cggtcagctg ccgccgcagt
     9481 acgtgccgcc tgctcgccgc cgaagttttt cgactgaccg ccaaacggac gttgatagat
     9541 acgaccatca tcaagacgcg agaacggcag gcccatatgt tccagttcca gaatcgcttc
     9601 cggcccggtt ttacacatat attcaatcgc gtcctggtca ccgatatagt ccgacccttt
     9661 tacggtgtcg tacatatgcc attcccagtt atcttcatgg gtattaccca gcgcaacggt
     9721 aataccacct tgcgcagaaa cggtatggga acgggtcggg aagaccttag agagcagcgc
     9781 acaggtctgg ccactctggg aaatttgcag cgccgcgcgc atacctgcgc cgccagcacc
     9841 aatcacaact gcatcaaatt ctctgactgg caatttcatc acacacccca caccacaacg
     9901 aatccataaa tcacgtaaac caccagtgca acgacaatca ccagttgcag catcaggcgc
     9961 aaggccagcg gtttaacgta gtcggtcaac acctgccaca tgccgatcca ggcatggatc
    10021 aagatagaaa acagcgccag cagggtgaac actttggtga acgcagaggc gaagaaacca
    10081 atccagactt catatgtcag ctcgccactg gtagcgaaaa aaccgaccat ataaatgatg
    10141 tagagcgtca ggacgatagc ggtagcacga acgaggatga aatcatgtac gccattgcgt
    10201 cctaatgcgg aggcgttgct taccatacga ggactcctgc gagaagtgaa agcacgacag
    10261 taataacaaa ggagattttg gcggagcgtt tacccgcttc gaatgtttct tccagatagc
    10321 caaaatccat catcatgtgg cgaataccta cgacgacgtg atatgccaga gcggtaagga
    10381 tgccccacat gataaatttg acgaagaagc tgcccataat cgcggaagct tgctcgaaac
    10441 cttcagggga agagaggctg gtacccagaa gccacagcag gatgcccact gcaacaaagg
    10501 tgatcacacc ggaaacgcga tggagaatgg acgctatcgc cgtgacgggg aaccggatgg
    10561 tctgtaggtc cagattaaca ggtctttgtt ttttcacatt tcttatcatg aataacgccc
    10621 acatgctgtt cttattattc cctggggact acgggcacag aggttaactt tctgttacca
    10681 ggagacgtcg ggatttcctt cctccggtct gcttgcgggt cagacagcgt cctttctata
    10741 actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac gacacgttac aacgctgggt
    10801 ggctcgggat tgcagggtgt tccggagacc tggcggcagt ataggctgtt cacaaaatca
    10861 ttacaattaa cctacatata gtttgtcggg ttttatcccg aacagtgatc tgcgtcacga
    10921 taacaacatt tatttaattt ttaatcatct aatttgacaa tcattcaaca aagttgttac
    10981 aaacattact aggaaaagca tataatgcgt aaaagttatg aagtcggtat ttcacctaag
    11041 attaacttat gtaacagtgt ggaagtattg accaattcat tcgggacagt tattagtggt
    11101 agacaagttt aataattcgg attgctaagt acttgattcg ccatttattc gtcatcaata
    11161 gatcctttac ctgcaagcgc ccagagctct gtacccaggt tttcccctct ttcacagagc
    11221 ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg cgaaggcaaa tttaagttcc
    11281 ggcagtctta cgcaataagg cgctaaggag accttaaatg gctgatacaa aagcaaaact
    11341 caccctcaac ggggacacag ctgttgaact ggatgtgctg aaaggcacgc tgggtcaaga
    11401 tgttattgat atccgtactc tcggttcaaa aggtgtgttc acctttgacc caggcttcac
    11461 ttcaaccgca tcctgcgaat ctaaaattac ttttattgat ggtgatgaag gtattttgct
    11521 gcaccgcggt ttcccgatcg atcagctggc gaccgattct aactacctgg aagtttgtta
    11581 catcctgctg aatggtgaaa aaccgactca ggaacagtat gacgaattta aaactacggt
    11641 gacccgtcat accatgatcc acgagcagat tacccgtctg ttccatgctt tccgtcgcga
    11701 ctcgcatcca atggcagtca tgtgtggtat taccggcgcg ctggcagcgt tctatcacga
    11761 ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc gcgttccgcc tgctgtcaaa
    11821 aatgccgacc atggccgcga tgtgttacaa gtattccatt ggtcagccat ttgtttaccc
    11881 gcgcaacgat ctctcctacg ccggtaactt cctgaatatg atgttctcca caccgtgcga
    11941 accgtatgaa gttaatccga ttctggaacg tgctatggac cgtattctga tcctgcacgc
    12001 tgaccatgaa cagaacgcct ccacctccac cgtgcgtacc gctggctctt cgggtgcgaa
    12061 cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg ggacctgcgc acggtggtgc
    12121 taacgaagcg gcgctgaaaa tgctggaaga aatcagctcc gttaaacaca ttccggaatt
    12181 tgttcgtcgt gcgaaagaca aaaatgattc tttccgcctg atgggcttcg gtcatcgcgt
    12241 gtacaaaaac tacgacccgc gcgccaccgt aatgcgtgaa acctgccatg aagtgctgaa
    12301 agagctgggc accaaagatg acctgctgga agtggctatg gagctggaaa acatcgcgct
    12361 gaacgacccg tactttatcg agaaaaaact gtacccgaac gtcgatttct actctggtat
    12421 catcctgaaa gcgatgggta ttccgtcttc tatgttcacc gtgatcttcg ctatggcacg
    12481 tacggttggc tggatcgccc actggagcga aatgcacagt gacggtatga agattgcccg
    12541 tccgcgtcag ctgtataccg gctacgcgca gcgtgatttt aagtcggata ttaagaagta
    12601 aaaatgacac cgacaaaacc ctgcgtggac acgcagggtt tttaattcta caccagcagc
    12661 aaagatattg ccggaatata ggtaatgacc aacagaagta aaataagact gatcagtaat
    12721 ggcaccgctt cacgcataaa ttgcccgaca gaaacctgta cggtattaca taccagcagc
    12781 atcaccgttc cgacaggtgg tgataacgtt cctaacgata agttgaggat cataatgatg
    12841 ccaaaatgca ccggatcaat gccaaaatga gccaccacgg gtttaagtaa tgggactaat
    12901 atcagcagaa tggcactacc ttcaaggaac atgccaagca ccagcagcaa tagattgata
    12961 atcaataaaa agacgtattt attatcggtc agagaagtta caaactccgc aaccgtgata
    13021 cctgcccgct caagggaaaa tatccagccc agcgcggcac tcgccataat gattaacatg
    13081 accgatgccg tggaacgcgc cgtctcagag agtgctgtaa atacatggct gattttcagt
    13141 tcgcgataaa ttacaaaacc aataaacatt accaataata ctgctaccgc tccggcttcc
    13201 gttggcgtaa agatatttgc ccgaatacca ccgataaccg caacaattaa gaacagtgct
    13261 ggccaggcct gcaccagcgc cactgacact tctttcggtt ttggccagtg ttcgctggaa
    13321 ggttttaaac cgcgacgaat agccaccaac catacggtaa cgatcaacat ggcggagcac
    13381 agcagacctg gcaggatccc ggccataaac atggagccga tggagacatc cgccaccagt
    13441 ccgtaaagga tcagtgcaat acccggtgga ataattggtg taatcagcga accacttgcc
    13501 gttatcgccg ccgcaaagcc acgggaataa cctttctgtt ccatttccgg caccatcatg
    13561 cgcgataaca ttgcggcatc cgccagattt gaggcactga caccgcccat catggtactg
    13621 accagaatat tagccccacc caggccaccg cgcattctgc caatcaacac gttggcaatt
    13681 tgcaaaatac gcgcggcaat accggtatag ctcattaacg tgcctaataa gataaaaaac
    13741 ggaatagcta acagcgaagg ggtttgcgtc gtggcaatca aacgctgaat tgccaccatt
    13801 aacggcacat ttgaatagca aaggaagtag gtcagaatgc cgcaaaacat cgccagccat
    13861 accggcatat tgataaataa acagacgaac ataagcggaa tgatcatcag ccagttatcc
    13921 agccagcttc ccgccagtaa ttcactcaat gctgtcatta ttcaccttct ttgcgtggcg
    13981 ttttcttgat cagttgtaag gcagtaaaac aggcgacacc gatagcgcca accgtgacgg
    14041 caatatcgat ccaaaaccag gatattttta atatttgcgt ggttttaaag gctgtcccgg
    14101 cagctaattt ccatgctgac caggccatga ccaccagcaa aataatagac atcagagaga
    14161 ctaaaaacgc taatatacgt tgcccgcgtg aagtaagtgc atcggctaaa aatggaatcg
    14221 ataaatgggt attatctcgt tcagcaacaa ccgcaccaaa catgactacc caaatcatta
    14281 ataaaccgga aacctcttct gtccactgta atggcatatg aaaacagtaa cgcattatta
    14341 ccgcagcaat ggtcatgatt accaatataa agagcgttaa taccgcagca acttcaatca
    14401 ttttccctaa aagacgcata aacacctctg ctcttaacgg tcggtgccga aacaccgacc
    14461 agtccttatg ccatgaagtt atttcagctg ttgctgaata gtgtcataca aaccaggggt
    14521 ccattccggg aattgttgat agacttttaa ggctttttct ttgaaagggg cgacgtcagg
    14581 atagatgatt tccacgccct gctcctgcat ggttttcaac attgtcgcat cctgctcagt
    14641 ggtaattttc tggctatata aaccagcttc atacgctgtc tggtgaataa tatctaactg
    14701 cgcaggtgtc agggtactga aaaacgcttc gccacccaac cataatgatg tattggtcag
    14761 atagttcacc attgacagat atttcgcctg ctcatgcagc ttttgcccat agagaacaga
    14821 aaccgggttt tccacaccat cgatcatccc ctgagtcagc gccggataaa catctcccag
    14881 cggcattggt gttggggttg cgcccatcgc ttcgatagct ttaatctgca tgacgttatt
    14941 cggcacgcgg atcttcatcc ctttcaagtc ttcaggtgta cgaatcggtt ttttagcaat
    15001 gatctgcctg gtaccgtaca gatagttgct catgacaata tgcacacctt tttcccgtag
    15061 tttctgttct ttttgtttaa accagtcact ggcataaatc gcaaacagtt tctgcggatc
    15121 ttcggttaaa taaggcccaa acaatattcc caagtctgca tcgtagtcag ttaaaaatcc
    15181 tacatcactg atggtgataa tattcatccc catcaacgct tgttcagtaa tatcttgttt
    15241 agaacctaac tgtgaacttg ggtaaagttc gaaaacaata tctccattgc tttttttctt
    15301 cacaagttct gcccagtgct tcactactgt atccgctggc tcgccggggt tactttcata
    15361 ggcgattttg atagttaccg gtgcggcatt cagcatcgga ctaaataaag ctaaaagaaa
    15421 taaaagtggt gtcagtaatt ttttcattat tattacctgt aagagcttta tcgattaacg
    15481 gggatctgcc cgacagactg cacttttgct gtaatcaaat gctttatacg ttaaaacaaa
    15541 ctcctgatgt tccagtcgtt ctgcggcaca atattgaccg ttggcgacat gttcaatcac
    15601 ctgcataata tcgtctgcaa tatcgtcgag cgttgcgcct tcgctaatta cacgaccggc
    15661 attaacgtcc atatcgtcag ataaatgctc ccacgtctgc gggttattac acactttaat
    15721 aataggagcg atcactgaac caataactga accgcgacct gtggtaaata acaccatatg
    15781 acagccagag gcgataagtt ccagtgcttc tgaggaatcg ttaatattcg gatacccaaa
    15841 acgaggttca ccatcaggca ccacgtccat caaccaaagc ccaggaaagg gcgtttgttg
    15901 cgtcggcaaa ataacgccag caatcagcgc atcgcccgac ttggtataag ccccaaggga
    15961 tttttcttcc tgagtggtta agccgccttc agcattaccg ttagaaaaac tccccagccc
    16021 catcaaactg taataacgtt cggcttttgt cattgccact tcgatggcat cacgtgcctg
    16081 cgaactagcc gcgcgcgaca tcatatgctg ctcacaacca agcaactcac cgctttcttc
    16141 aaaaatacag gtagcccctt tctccagcag ttggtcaaag gcacgtccta ctgccgggtt
    16201 acccgtgata ccactggtgc catcggagcc accgcagata gtaccaacca ccaggtcgct
    16261 ccagttcagg atacaacgcg gtgtatccgc aagctgttcc agcatttcag cgaccaactg
    16321 cttacccaga gtaatggtgt tggtcgtgcc tttattttcc tgaatgacca gcgtatggca
    16381 cggtcgccca ctttctcgta cttcttgctc cagccgacgg cgatcgaaat tctcgcaccc
    16441 cagcgaaata atcagtacac cacctacgtt gctatgagtc gccagatttt tcaccagccg
    16501 atagccataa tcgctgggat aacaacctga aaaaccaaaa tactgcacgt cgtgacaagg
    16561 aaatccttcg gcaatctttt tagcgacatg gctggcacac tccaccataa agattacctg
    16621 cacagtatta cgaatacctg gtcgaccatt ttgccgtaca taaccttcca ttatgctttt
    16681 tcctctgact gatagccggc atcatcatga tgaaaaatgg cgatggcatt gctggcaaga
    16741 ttatgggaat gcacatgttc acccatggca atatcgcgag tggcgtgtcc gataatggcg
    16801 ccgtatttca gaatcgaatc accattgttc agcgcttcgc aggccagttt gtgccccagg
    16861 gtaacggaac tacaggccac catttccccc tcatcccacc agacaatatc gccactggcg
    16921 atgtcattca gcgcgactac acagttatct ttggcatgta gctttaacgc acgtttgcgc
    16981 ataattcctc cgtacatcag aagccgatca atgcgccgcc atccaccacc agcacctgac
    17041 cactgacaaa cgccgctgcg tctgaagcca gataaaccgc agcccaaccg atatcctccg
    17101 gcttgccgaa ttttttcatc ggggtacgac ccaggatttt gttacggcgt tcgtcatcac
    17161 cttcaatggc cttacgcaac attggtgtat cgatccagcc tggcgcaata ccattgacgc
    17221 gaatcccatg aacggcaagc tccgtggtca taccgcgaat catgccgaga taggctgact
    17281 tcgctgcgct gtagccagta accagaggct gcccaagaaa tgaggtcatc gatgcagtaa
    17341 aaagcacaca gcctttaccg cgttgtttca tatgcggcac cagcgctttg gttaacgcaa
    17401 atgcgccgac tacgtgaacg tcgagtactg actcaaaatc actgacgctc atctcttcaa
    17461 tcggtttttt gcagtgattg cctgcgttat tgaccaggat atcgatagcc ccgtgttcgc
    17521 taataagcac atcaactttt tcctgcgcgg ctgcggtatc cccgacatcc atttgtatcc
    17581 aactggcctg cgaccccaat gttccggcgg tcgcttgcgc cagctcgccg ttcaggtcag
    17641 caataatgac cttcgccccg ctgactatca tgcatttcgc cattgccagc cccagcccac
    17701 tggctgcacc ggtgatcaac gcggttttgt tatgtaaaga aaaagcattc atggtgtcgc
    17761 tccttaaagg tcaatgagga ttttggtata cgcgccggga tcacttgccc agtcgctaaa
    17821 agcgtgatca gcctgttcca gcgggtagtg agcactcacc agattgctga ccggataacg
    17881 cccggactcc atcaatgcca gcacggcacg aaaatccgcc acggtggcat tgcgcgaacc
    17941 acggatatcc agttctttca tcaggaactg cgcactctga taactcactg gctgtttggc
    18001 gtaaccgaca taaaccacac gaccgctaaa cgccaccaac cctgtagcct gttcaatggt
    18061 ttgttgatta cccaccgctt caatcaccac ttccgggccg cgaccgtcat ttaacagcac
    18121 cagttctgcc gccacgtcac attgctgcga attaagaaaa cgttgcgcac cgcattccag
    18181 ggcaatgttc cgcttgcgat catcaatatc gatagcaatt acgcgcgccc ccatggcgga
    18241 tgccgcggcg atagcgccaa ggccaattac cccacaaccg ataactgcga cccattcacc
    18301 cacattaatt tgcgcacgtc tggccgcatg tagtcccacg gccagcggtt ctaccagcgc
    18361 taactcatca acacttaagg cattacagac aatgacttta tgtgcaggca tcgtcagttg
    18421 ttgcgccatc cccccctgac gttgcacacc aagagtttgg ttgtttttac aggcgttagg
    18481 acgaccgctc agacaggagc tgcacacgcc acactcagtg caaggcagaa cgaccacccg
    18541 gtcacctggc ttgatcgccc ctgccatatc gccagttgcc agcaccgtgc cgccaatttc
    18601 atgccccgga atacagggat aattaaccag cggatttagt ccccgccagg tattgaggtc
    18661 agaaccacac agtccgacac gctcaacatt gattagcact tctcctttac cgcagacagg
    18721 ttcggggata tcaacaaatc gcgcctgact tccctgctca atggttaatg ccttcattca
    18781 ttgctctcct tacattacgc cgaaggtttc aaaggcctgt accgtgctca tgccatcact
    18841 gatggctttg cgcacttcac tttcagttgc cactttttcc agggcctggg taataacttc
    18901 tttctcaaca acctggggaa taatcagtaa tccgtcacga tcaccaaaca cgatatcgcc
    18961 cggattcact cgcacgccgt gaaattcaat aggtacgccg aagtcgatga ccttgccgcg
    19021 cggaccttga tcctgtgcgt aagtgccatg acaaaaagtg ggaaatttca attcaagaat
    19081 ttcagcagag tcacgggcgt aaccatcgag taccgcacct gctgctttca gatgcatggc
    19141 acgggtagac atcaacccgc cccacaaggc atagcgcaga gagctgcccg tagtgatgta
    19201 aacatcgttc tctttgaggg catccagcgc ttcaaacatg atgccaaacg gtttttcgcc
    19261 aatcgccgtc ttacccgacg tcgatttggt ttcgtaaacg tccgactcca gcaccggcat
    19321 tgcccggcct gccaccaccg tattcacatc cagcggcttg attgccggag aaacaaactg
    19381 gtgcatatag cccagcgtgt cgagaatatc gccaaccacg gcggtaaata gccgggtttt
    19441 catcaaagca aaaagttcgt tatcactctt ccacattatt ttcgtcctgt aagcctgttt
    19501 aacaggttta cagggtaagg agaaaaagcg cacaatactg cgaagggcgt cacgaaaaag
    19561 ttgtgtattt actatttctt cgccactctt tcacacaata tgtctttgat tcgcactgca
    19621 tggcgggcgc acaggcgcag tagtctattg ataatcaagg gttctggagg aaaatatgaa
    19681 ggatacagtc ccggtattct ggatttcacc gaccaggaaa aagtacaaca ggagtaaaaa
    19741 tgagtgagag tgcgttattg accagcgtaa cgttatcgtc acaggtcaaa aatcatattc
    19801 ttaatctgat cgccgaacaa aaattgacac cggggatgcc agttcccagt gaagttcagc
    19861 tgattcagaa attgggtgtc agtcggggta ttgtgcgtga ggcctatcgt tcacttgccg
    19921 ccctggggat tctggaaacg cacagcggta aagtgccacg aatcaaaaaa tttgatgatg
    19981 aagtgctgcg cgtggtgttt ggatttgccc tggcgaccga acaagtgaca acagagcaaa
    20041 ttcttcaagt acgccgctgg ctggagttgg gcgttgtgga agctgctgcc cgtaatggcg
    20101 aagaggaaga ttttgctaac ctacgggctg aaatggcaaa aatccgcgaa cagttcaatg
    20161 atgttgaaag ctttatcacc catgatatcc gcttccatca gttacttgcc gttgcctcaa
    20221 aaaatccgct ttttatgatg ttattgcagg cattacatca gcagttgcgg caatccatcc
    20281 aggagggttt aaacgcccaa agcgatcaga aagagtatgg caatcacatc atcacactac
    20341 accagaaaat ctgcgatgct gtgtgtgcca gggatgtcga aacggcgact caagccatga
    20401 aatcacactt cgacagcccg gtcagtgcac tgatcaataa ttcctcgtca taagacttat
    20461 ctcagttcaa tcgtttactc ttccctcttt tacaaacgag agggagacaa cggtacttaa
    20521 tacgcccttt agccaaaatc tgtctttttc ttgattaccg ctattttttc gttgaggcgc
    20581 attctttatg ctgtatcgat acaccaaagg taagttaaac agggagtgac ggatgccagt
    20641 gctacagtgg ggaatgttat gtgttctgtc acttctcctt tctattggct tcctcgcttt
    20701 acatctcccg gcggcactcc tgcttggccc gatgatcgcg gggatcatct tcagtatgca
    20761 cggagtcact ctgcaacttc cccgctccgc ttttctcgct gcccaagcca ttcttggctg
    20821 catgattgca caaaacctca ctggttcgat tctcaccacg ctggccgtca actggccgat
    20881 cgtgcttgcg attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt
    20941 gcgctatagc tcactgcctg ggaataccgg ggcctggggt tcttctcctg gcggcgcggc
    21001 ggcaatggtc gcaatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca
    21061 atatctgcga gtgctgttcg tcgcaggagc cgctgttctg gtcacccgca tgatgctggg
    21121 cgataacgct gaagcggtta accagcagat cgtctggttc ccgccagtga gcattaatct
    21181 cctgcttacg attttgctcg cggtcgttgc cggtacggcg ggatgcttgc tacgtcttcc
    21241 ttccggcacg atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat
    21301 cacgattgaa ctcccggaat ggctgctggc gatggcatat atggcaattg gctggcggat
    21361 tggtcttggt ttcgataagc aaatattact gcgggcattg cgcccgctac cgcaaatcct
    21421 gctgtcgatt tttgctctgc tggctatttg tgcgggcatg gcgtgggggc tgacccggtt
    21481 tatgcatatt gattttatga ccgcctacct cgccaccagc cccggcgggc ttgataccgt
    21541 ggcggtcatc gccgcaggga gcaatgccga tatggcgctc atcattgcga tgcaaaccct
    21601 gcgcctgttc agtattttgc tgacggggcc tgccatcgca cggtttattt caacctatgc
    21661 gccgaagcgt tcggcctagt gctggcagcc tgggcaccag taaaacgggc gcgatgacag
    21721 cgtggttttc tcaatgatgc cgccacagcg ttcgcaaggt tcgccatcac gatgaaaaac
    21781 cttaaagcga aacagcgccc catgatattt gttttcatcc acctgccccc gcgtagcgta
    21841 agaaagtcgc ggaatatcca gtaacgcgtg tgcgagtgca tccagttgcg gcgcattgag
    21901 atctttcgct ttatgatttc cggtcaaccc aacctgccag aggatctcca cccgcaaata
    21961 attgccaagt cctgccagaa acgcctgatc gagcagtaat ccagcaaact gacggttacg
    22021 aaagcgcggc gacaataatc gttcctttac cacctccggc gtcagattcg gatccagcac
    22081 atcgggacca acgcgttgta aaaacggatg cgtggtcagt tgttccggag tcaacatctc
    22141 aatatccgag gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa
    22201 tactcgcgtg gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg
    22261 attatggctg tagagcgtta agtcgttgga aaaatgagtt aacaacgcct taccacgcgt
    22321 ttccacatgg gtaacgtgtt gaccgataag tcgtgattga taagatttta actgcgggaa
    22381 ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc
    22441 acggcggatc tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg
    22501 ggttatgctg cgacaacaat ccccttttca gcaaatgtag ctcgtaatcg gcgggcgaac
    22561 gccagtgcgt gctcgccatc gccatgtagg cagaccgttt gcgccgtgac cattgcccat
    22621 tcgccggtga tacttttgac tctgccgtgt tgcaccatct ccaacgtttg cgccagcgcc
    22681 tgttcttcgt tttcaatcaa cgcgcctggc tggcttcgcg gcaccagcga accgtctgcc
    22741 tgataaccac gatcggcaaa cacttcctcg cgcgttacca ggccatatcg ttcgcctgca
    22801 cgaatcagct cgcttcctgc cagcccgacg agaatcaatg ccggatcgca agcgtatacc
    22861 gctctggcga tagcgtctgc cagttgtgcc tctttcgccg cctggttgta taacatgccg
    22921 tgcggtttga catgacacat tacgccgcct tgcgcacggg taatcgctgc cagcgcgcca
    22981 atttgataca gcgtctgggc gaaaatggtt tctggcggca gctgcatggc gctgcgacca
    23041 aaattttccc tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct
    23101 tcacgcacgc aagcctgcat ggtttgcgca tctcctgcat gaaatccaca ggcaatattg
    23161 gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg
    23221 ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt tcgaaataac gttgctgatc
    23281 ttgccgcgct ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag
    23341 cggaatttgc gccagatggt acatatcagc ctcaatgata caggcaatac gcgggtaacc
    23401 accggtggtc tgtgcgtcgt tcatcaatac aatcggctgg ccgttatgcg gcacctgcac
    23461 cacgcccggc aacaaaccgt gagataacag ttcgcgatcg gtggtgcgtt ttaaaatttg
    23521 cccctgtaag cgatagccca tgcggttact ttgcgggcta agctgccagg gcgaacgcca
    23581 gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat
    23641 gcggttgccc cagagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgcttt
    23701 gccaatcggg agtcggtcac catccctcag taaacggcct tccagcccgc caatccccac
    23761 tttgagatcg gtgctgcatg atcccattac cggcggaaca tcaataccac ccgcgaccgc
    23821 cagataactg cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat
    23881 cggcaatcgc cagccggtcc agacggcatt atcatccagc cgcgcttcgc aaccggcacc
    23941 cgtcagagca aaccacccat cagtttcgaa ctcaaccgtt aactgaccga gcgtgatctc
    24001 cagcgcgggg gcattggcgt cattacccac cagcaggtta gcaatgcgta acgcaggcat
    24061 atccagtgcg ccacagtggc tgataccaga ctgacgaaag ccgtgacggc cgccatcctg
    24121 cacggtggtg tacatgcccg cacgaataat cttcagcata ctccctcctt ctgcggtaca
    24181 aagcgcacgc tgtctcccgg acgtaataag atgggttcgt cacacgccgg atcaaacagg
    24241 ctgagtgagg tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacaccg
    24301 gtctgcggcc cgccgatccc gacagaacct gccggaacgt gcaagcgcgg ttcagcgcgc
    24361 cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct
    24421 aaaaaccaga ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaat
    24481 ccacaatgcg ccgcgaccac cgccaaatcc ggtccggctg cgccaccgta aactaccgga
    24541 atttcaataa agcgagactc cggctccagc gcctcactct cctcccacca gcgttgcaaa
    24601 cgctctatgg catccagcgc cagcgactca ggattacgca aaatcactgt gatattgttc
    24661 atgccaggaa tggcttcaac cacattcggc atatccacca gacgctgtgc cagtcgccag
    24721 atccgtttct ggctagccag cgtcaccggc ggttccagtt ccagtactac cgccgtttca
    24781 cctatcagat aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg
    24841 attaggaata tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa
    24901 tgcgcgaata ccaccgcgtt cagtggcatg gtgacccgca gcataaaaat gcaatccctg
    24961 ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc
    25021 aaaacgcgcg gcgctatcga taaaactttg cccacccccc gtgcaccagg cgacgcgctg
    25081 gaccacctca ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat
    25141 ccaggaggcc agttccagtc ccggcacagg catggtcagt tcgccccacg gcaccaacgg
    25201 ctcaatttcg cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattacccag
    25261 ctccggatgc gcgtccaggg gcagatgcca gccatacagg ttgatatcat tcgccagcaa
    25321 cgtttttaaa cggttacgct tcattccgcg aatgaccgga gattcacctt tccagaagta
    25381 gccatgatgc acaatgaccg catccgcgcc cagacgcact gcctcatcga gcaaagcctg
    25441 gctggcagtt acaccggtaa caattttttg caccatctct ttgccttcca cctgcaaacc
    25501 attcggcgcg taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag
    25561 ttcggtgttt ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt
    25621 tacctcgcct gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca
    25681 aaagacgcaa attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca
    25741 gcatattgca taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac
    25801 gccgcgcaac tattacgtga cgttgcagtc aattgtggaa ttattatgaa taaacacgca
    25861 tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt
    25921 tacggcatgc gtgccctgct gatcctctat ctcaccaatc aactaaaata tagcgataat
    25981 cacgcctacg agttatttag cgcctactgt tcgctggtat atgtcacgcc aatcctcggt
    26041 ggctttttgg cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg
    26101 atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg
    26161 tccctggcga ttatcgtctg cggctatggt ctgtttaaat ccaacgtcag ttgcctgctc
    26221 ggtgagctgt atgagccaac cgatccacgt cgtgatggcg gtttctcgct gatgtatgcg
    26281 gcgggtaacg tggggtctat tatcgcacct atcgcctgtg gtttcgccca ggaagagtac
    26341 agttgggcga tgggctttgg cctggcagcg gtgggtatga tcgcgggtct ggtcattttc
    26401 ttatgtggca atcgtcattt cacacatacc cgcggcgtta acaaaaaggt actgcgtgcg
    26461 acaaactttc tcctgcccaa ctggggatgg ctactggttc tgctggtggc aacgcctgcg
    26521 ctgattaccg tgttgttctg gaaagagtgg tcggtatacg ccttgattgt cgcgacgatc
    26581 attggccttg gggttctggc aaaaatttat cgcaaagcag aaaaccaaaa acagcgtaag
    26641 gagctggggc tgattgttac cctcaccttc ttcagcatgt tgttctgggc attcgcacaa
    26701 caggggggta gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt
    26761 tataccgttc cgactgctat gttccagtcg attaatgcct tcgcagttat gctgtgcggt
    26821 gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg
    26881 ggaaaatttg ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc
    26941 gcccgctggt ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctggcg
    27001 gtgatgggct ttgcggaact gtttatcgac ccggttgcta tgtcgcagat tacgcgcatt
    27061 gaaatccccg gcgtgaccgg cgtattaacc ggcatttata tgctgctttc cggcgcgatt
    27121 gcgaactatc tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc
    27181 ggggcgatca actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc
    27241 gcactggcgt gtgtaggact ggtactgatg atttggctgt atcaggcgct taagttcaga
    27301 aaccgcgcgc tggcgctgga gtcttaataa gccagcacca tgcctgataa aacgctcgct
    27361 gcgttttatc aggctccttt cctcgcttct tcgtacgctg ccagcgttcg cagccttgcc
    27421 tctttgtgat cgactatcgg ttgcggataa tccaacgtca cacctgtttt ctctgcccac
    27481 ttccacggct catgcaccgc tttccccggt acatcgcgca gctccggtag ccactggcgg
    27541 ataaattcgc cttcgcggtc aaatttctcg ccctgagttg tcgggttgaa aatacgaaaa
    27601 tacggcgctg catcagtacc cgtagaagcg gcccactgcc agccaccgtt attagctgcc
    27661 aaatcaccat caatcagctg cgacatgaaa tatcgctcgc cttcgcgcca gtcgatcaac
    27721 aaatctttca ccagaaaact ggctgtaatc atccgtagcc ggttatgcat ccagccagtg
    27781 ctgttaagct gacgcatggc agcatcgaca atcgggtatc ccgttttgcc ttcctgccag
    27841 gcctttaaat gggcgggatt actttgccac tgtacacgat ccgtccaggc aataaacgga
    27901 cgatgtttac acagcgaagg ataatacgtc atcaaatggc ggtagaactc gcgccagatc
    27961 agctcactaa gccagacact accggctcca ccgtccagcg cctgcggctg ttccgccaac
    28021 aagcgatgca aacactggcg aggcgataac ccgcccgttg ccaggctggc ggacaaacga
    28081 ctggtgcctt ctactgccgg aaaatctcgt tgttgctcat actctccggc accgttctgg
    28141 caaaactggc gtaattgcgc aatcgccgct ttttcttcca ccgggaaatg cgcagtatcg
    28201 aaagactgac gaggataatt cagcgtaatg gatggcgcgg gatctatcgc tccactacta
    28261 cgtacttttg gcgcagcgac gcactccggc atcccttccc gcagccgttt cagccaggca
    28321 ttcttaaaag gcgtaaagac tttgtacatc tcatgattgc cagtcatcac cgcccccggc
    28381 ggcaggatca cgctgtcatc aaatccttca cacaccacgt tacgcagcgc tctttcaact
    28441 tccacatccc gcgcccgctc atttacctca tactgatagt tataaaaaag atgagtaacg
    28501 cggttttccg cgcacacctg tttaactatt tcgacgctgg cggcaaagtc atccacttca
    28561 cggaacaata aaggaatacc tttttccgcc agcgcgtttt gcagcgcatt aagttgtgta
    28621 ttgataagtt cagcctgacg cggcgacaca ttatgcgccg cccactggcg tggggtagcg
    28681 atatacaacg ccagcacgcg tgcagacgaa ttgcggcagg cggcagccag tgcgagatta
    28741 tcgtgcagac gtaaatcctg gcgaaaccag accagatggg tagtcataaa actcctgcat
    28801 ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg ctgccctgaa agccaggcgt
    28861 caggataaag cgccatatag tgtttgatac gcatcagcgg cgcaagaagc gattgctcgc
    28921 cacggcgata actgtcgata agcgccgcca gctgctggcg ttctgtggaa tcaatatgtg
    28981 ggcgaaaata tccctgaacg tgcatcaaaa cgttagtatg atcacgccgg ttggcagggt
    29041 gagaaagcaa aacaatcacg cgttggcggt actggttgta atagtcatcc agactttgcc
    29101 accggtgaat atcagccaca aacgggccaa gtttgcggta ctccggctga gaatgcgcca
    29161 gaaagacgag tttatagcgg ctgtgataat cgagaagcgc cccgcgcgtt agtcctcgat
    29221 ctttgagatt gtgtaactca tgcaaagcaa aaatgcggat aagggtgctg tcatcaaaaa
    29281 gttgttgatt cattactgct cctgaacggt ttacaggagt aagtgtagac gccgaaaaaa
    29341 gaaaaccgcc agcgtttcca ccggcggttt tctttaaacg tcatgtttgg caatcagtaa
    29401 aaatcaccgg ttgccttttc ggcctgatcc atccacaccg gtttatcgct ggttttcgac
    29461 cagacgcggt gcaggtagct ataaaagcgg gcgcggtctt tccagaaaag catgaaaggc
    29521 agcgccagaa cgcccgctgc aaccgcacaa gtacggcgta aaaagataag ccatgcagga
    29581 tattctttgt agagttccat acttttctcc cccttttgta tctacccggt gaatagcacc
    29641 ggaaaaatga attcgtttat ctgatgaaaa tagtaccgct gtttgtgtaa ttttactact
    29701 catccgacca cttatttttg cttattgatg gtttatttat attcaccgtg taattaagtt
    29761 acataaaagt taaattaata ctaaacatta gttaaatcat tgcttttgcc atttttatac
    29821 tttttttaca ccacgcccgc agatttttgc gaaatctttg cagccagaac tctacccttc
    29881 cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtgt
    29941 tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcattct
    30001 gatggctgcg caagggttct tactgattgc cacgttttta ctggtattaa tggtgctggc
    30061 gcgtccctta ggtagcgggc tggcgcggct gattaatgat attcctcttc ctggtacgac
    30121 gggcgttgaa cgcgtactct ttagcgcgct tggcgtctct gaccgcgaga tgaactggaa
    30181 gcagtatctc tcagccattc ttggcctgaa tatactgggg ctggcggtgc tgttttttat
    30241 gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccagggc tgtcgtggga
    30301 tctggcgctc aataccgccg tcagctttgt caccaatacc aactggcaat cttatagcgg
    30361 tgaaaccacg ttgagctatt tcagccagat ggcgggctta acggtacaaa actttctttc
    30421 tgccgccagc gggattgcgg tgatttttgc cctcattcgt gcatttaccc gccagagcat
    30481 gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg tgctagtccc
    30541 tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt gcgctgcaaa actttctgcc
    30601 ctatcaggcg gtgaataccg ttgaaggagc acaacagctg ttacccatgg ggccagtagc
    30661 ttctcaggaa gcgatcaaaa tgctcggaac taacggcggt ggctttttta atgccaactc
    30721 gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcaaatgc tggcgatttt
    30781 cttgatccca acagcgctgt gctttgcctt tggtgaagtg gcgggcgatc gtcgccaggg
    30841 gcgcatgttg ctgtgggcga tgtcggtgat ttttgtcatc tgcgtaggcg tagtgatgtg
    30901 ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcggacagca gcatcaatat
    30961 ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac
    31021 ggcggcttcc tgcggcgcgg tgattgcgat gcatgattcg tttaccgctc ttggtggcat
    31081 ggtgccgatg tggttgatgc aaattggtga agtggtattc ggcggcgtcg gttccggcct
    31141 ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg
    31201 tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga ctgcactggc
    31261 gattctggtc accccgacgc tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc
    31321 cggacgtagc gccatgctca accctggccc gcatggtttt agcgaagtgc tgtacgccgt
    31381 gtcatccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actctccatt
    31441 ctggaactgt ttactggcgt tgtgcatgtt tgtcgggcgc ttcggggtga ttatcccggt
    31501 gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct ccggcacatt
    31561 gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc
    31621 actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct cctgatgata
    31681 ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgtcg
    31741 ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg
    31801 tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc gcgatggcaa
    31861 gcggtgtgat gcctggtaat gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca
    31921 ccgtactgtt cgctaatttc gccgaggcgt tggcagaagg ccgcagtaaa gcgcaggcca
    31981 acagtctgaa aggggtgaaa aaaaccgctt ttgctcgcaa gctgcgcgaa ccgaaatatg
    32041 gcgctgtggc agacaaagtt cctgccgatc aactgcgtaa aggcgatatc gtactggtag
    32101 aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca tcggtcgatg
    32161 aaagcgccat caccggggaa tcggctccgg tgatccgtga atccggcggt gattttgcct
    32221 ccgtcactgg cggcacgcgt attctttctg actggctggt gattgagtgc agcgttaacc
    32281 ccggcgagac atttctggat cggatgatcg cgatggtgga aggtgcgcag cgacgtaaaa
    32341 cgccgaacga gattgccctg accattctcc tgattgccct gactatcgtc tttttactgg
    32401 caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgcagtcagc gtaacggtac
    32461 tggtggcact gctggtctgt ctgatcccaa ccactattgg cggcctgttg tcagcgattg
    32521 gcgtcgccgg gatgagtcgg atgctgggcg cgaatgttat tgccaccagc gggcgtgcag
    32581 ttgaagcggc gggcgacgtg gacgtcctgc tgctggataa aaccggcacc atcacactcg
    32641 gtaaccgtca ggcgtcggag tttatccctg cgcagggcgt ggatgaaaaa acgctggctg
    32701 acgccgcaca actggcttcg ctggctgatg aaacgccgga aggccgcagt attgtgatcc
    32761 tcgccaagca gcgttttaac ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg
    32821 taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc atgatccgta
    32881 aaggttctgt cgatgccatt cgccgccatg ttgaggctaa cggcggtcac ttccctgccg
    32941 atgttgatca aaaagtcgat caagttgcgc gtcagggagc cacgccgctg gtggtggtgg
    33001 aaggttctcg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag
    33061 agcgcttcgc ccagctgcgt aaaatgggca ttaaaacggt gatgattacc ggcgataacc
    33121 gtctgactgc cgccgcgatt gccgcggaag cgggcgtcga tgattttctc gccgaagcaa
    33181 caccggaggc caagctggcg ttgattcgtc agtatcaggc agaaggccgt ttagtggcga
    33241 tgaccggcga tggcaccaac gatgctccgg cgctggcgca ggcggatgtc gcggtggcga
    33301 tgaactccgg cacccaggcg gcgaaagaag caggcaatat ggtggatctc gactccaacc
    33361 cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat gctgatgacc cgtggctcgt
    33421 tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccagcggcgt
    33481 tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact
    33541 ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg attcccctgg
    33601 cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat
    33661 ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac
    33721 tgctaaccgt ttgcggtctg gtgtgaggtt taccatgagt ggattacgtc cggcattatc
    33781 gacatttctc tttctgttat tgattactgg cggcgtttac ccgctgctga ccaccgcact
    33841 ggggcaatgg tggtttccct ggcaggccaa cggttcattg attcgtgaag gtgatacggt
    33901 gcgcggttcg gcattaatcg ggcaaaattt taccggcaac ggctattttc atggtcgccc
    33961 gtcggcaaca gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcagt
    34021 cagtaacccg gtgctggata aacagatagc cgcacgcgtt gccgcattac gggccgctaa
    34081 cccggacgcc agcacgagcg ttccggttga actggtgaca gcgtcagcaa gcgggctgga
    34141 caataatatt accccgcaag cggcggcctg gcagatccca cgcgtggcga aagcgcgtaa
    34201 tctcagcgtt gaacagctca cgcaactgat cgcaaaatac agccaacaac cgctggtgaa
    34261 ctatatcggc cagccggtcg tcaacgttgt agaactcaat ctggcgctgg ataagcttga
    34321 tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact gccgcgccgc
    34381 atcgggggaa gctgaaagtt ttcttcggtg cctgcgcagg cgtcggaaag acctgggcga
    34441 tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag
    34501 aaacccacgg gcggaaagat accgccgcaa tgctggaagg tctggctgtt ctgccgctga
    34561 aacgccaggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc
    34621 gccgtccggc actgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc
    34681 atcctaaacg ctggcaggac atcgaagagc tgctggaagc aggcattgat gttttcacta
    34741 ccgtcaacgt tcagcatctg gaaagcctga acgatgtggt cagtggcgta accggaattc
    34801 aggttcggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgta gtactggtgg
    34861 acttgccccc ggacgatctg cgccaacgac tgaaagaagg caaagtctat attgccgggc
    34921 aggcagaacg cgccattgag cattttttcc gcaaaggcaa tctgatcgcc ctgcgcgagc
    34981 tggcgctacg ccgtactgcc gatcgcgtag atgagcaaat gcgcgcgtgg cgggggcatc
    35041 ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc gggcataaca
    35101 ccggcagtga gaaactggtc cgcgcggcgg cgcggctggc gtcgcgcctg ggcagtgtct
    35161 ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggagaaa aaacgtcggg
    35221 caattctcag cgccttacgt ctggcgcagg agttgggcgc ggagacggca acactttctg
    35281 atccagcgga agaaaaagca gtagtgcgtt atgcccgtga acataatctc ggcaagatta
    35341 ttctcggtcg cccggcctcg cgccgctggt ggcgtcggga aacgtttgct gaccggctgg
    35401 cgcgcatcgc ccccgatctc gatcaggtac tggtcgcgct tgatgaacca cccgcccgca
    35461 cgattaataa cgcgccggat agccgctctt ttaaagacaa gtggcgggta cagattcagg
    35521 gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg cagtggctga
    35581 tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtt gtggtggcac
    35641 tattttacgg acgctggcct tcggtggttg cgaccgtcat taatgtggtg agtttcgatc
    35701 tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct
    35761 tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgctggc gtgcgttatc
    35821 aggcgcgggt agcccgttac cgcgaacaac gcacacggca cttatatgaa atgtccaaag
    35881 ctctggctgt gggccgtagt tcgcaggata tcgccgccac cagcgaacag tttattgcct
    35941 ccacgtttca tgcccgtagc caggtgttgc tgcctgatga caacggtaaa ttgcagcctt
    36001 taacacatcc gcaaggaatg acgccgtggg acgatgccat cgcgcagtgg agttttgata
    36061 aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg tgtaccgtac cagattttgc
    36121 cgctaaaaag cggcgagaaa acctacggac tggtggtggt ggaaccgggg aatctgcgcc
    36181 agttgatgat cccggaacag cagcgcctgc tggagacgtt tacgctgtta gtcgccaatg
    36241 cccttgagcg actgacactg accgccagcg aagaacaggc gcggatggca agcgaacgtg
    36301 aacagatccg caacgccctg ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg
    36361 tgctgttcgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcgccccacg
    36421 cccgccaggc cagcgagatt cgtcagcatg tgctgaacac cacccgactg gtgaataatc
    36481 tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc
    36541 tggaagaagt ggtcggcagc gcgctgcaaa tgctggaacc gggtttatcg tcgcccatca
    36601 atctttctct gccagagccg ctgaccttaa tccacgttga cgggccactc tttgaacggg
    36661 tgctgattaa cctgctggag aacgcggtga aatatgcggg ggcgcaggcc gaaattggta
    36721 tcaatgccca catcgagggc gaaaatctac aactggatgt ctgggataac ggccccggtc
    36781 ttccaccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg
    36841 cggtaccggg tgtagggctt gggttggcaa tttgtcgggc gatagtggat gtacacggcg
    36901 gcaccattac cgcattcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc
    36961 agcaaacagc ccctgaactt gaagaatttc atgaggatat gtgacaaacg ttctgattgt
    37021 tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg atgggatgcg
    37081 cgtctttgag gccgaaacgc tgcaacgcgg cttgctggaa gcggctaccc gtaagccaga
    37141 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct
    37201 gcgccagtgg agcgcggtgc cggtgattgt gctttccgca cgtagcgaag agagcgacaa
    37261 aatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg gcattggcga
    37321 attgcaggcc cgtctgcgcg tcgctttacg tcgccactcc gccaccgccg cgcccgatcc
    37381 gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc cgcgttatcc atcggggcga
    37441 ggaagaggtg catctcaccc cgattgagtt ccgcctgctg gcagtgttgc tcaacaacgc
    37501 cggaaaagtg ctcacccagc gccagttact caatcaggtg tggggaccaa acgcggtcga
    37561 acacagccac tatttgcgta tttatatggg gcatctgcga caaaaactgg agcaagatcc
    37621 cgcccgcccc cgccatttta ttactgaaa
//
LOCUS       DAPQZD010000031        37478 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPQZD010000031 DAPQZD010000000
VERSION     DAPQZD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.031"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            57..1001
                     /gene="leuO"
                     /locus_tag="RO454_001506"
     CDS             57..1001
                     /gene="leuO"
                     /locus_tag="RO454_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDY0933354.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1162..1200)
                     /locus_tag="RO454_001507"
     CDS             complement(1162..1200)
                     /locus_tag="RO454_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933355.1"
                     /translation="MKRNDKILHYRG"
     gene            1319..3043
                     /gene="ilvI"
                     /locus_tag="RO454_001508"
     CDS             1319..3043
                     /gene="ilvI"
                     /locus_tag="RO454_001508"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDY0933356.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGARFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3046..3537
                     /gene="ilvN"
                     /locus_tag="RO454_001509"
     CDS             3046..3537
                     /gene="ilvN"
                     /locus_tag="RO454_001509"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDY0933357.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3549..3635
                     /locus_tag="RO454_001510"
     CDS             3549..3635
                     /locus_tag="RO454_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDY0933358.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3717..4721
                     /gene="cra"
                     /locus_tag="RO454_001511"
     CDS             3717..4721
                     /gene="cra"
                     /locus_tag="RO454_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDY0933359.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            5323..5781
                     /gene="mraZ"
                     /locus_tag="RO454_001512"
     CDS             5323..5781
                     /gene="mraZ"
                     /locus_tag="RO454_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDY0933360.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            5783..6724
                     /gene="rsmH"
                     /locus_tag="RO454_001513"
     CDS             5783..6724
                     /gene="rsmH"
                     /locus_tag="RO454_001513"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDY0933361.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            6721..7086
                     /gene="ftsL"
                     /locus_tag="RO454_001514"
     CDS             6721..7086
                     /gene="ftsL"
                     /locus_tag="RO454_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDY0933362.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            7102..8868
                     /gene="ftsI"
                     /locus_tag="RO454_001515"
     CDS             7102..8868
                     /gene="ftsI"
                     /locus_tag="RO454_001515"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDY0933363.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            8855..10342
                     /gene="murE"
                     /locus_tag="RO454_001516"
     CDS             8855..10342
                     /gene="murE"
                     /locus_tag="RO454_001516"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDY0933364.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            10339..11697
                     /gene="murF"
                     /locus_tag="RO454_001517"
     CDS             10339..11697
                     /gene="murF"
                     /locus_tag="RO454_001517"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDY0933365.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            11691..12773
                     /gene="mraY"
                     /locus_tag="RO454_001518"
     CDS             11691..12773
                     /gene="mraY"
                     /locus_tag="RO454_001518"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDY0933366.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            12776..14092
                     /gene="murD"
                     /locus_tag="RO454_001519"
     CDS             12776..14092
                     /gene="murD"
                     /locus_tag="RO454_001519"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDY0933367.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            14092..15336
                     /gene="ftsW"
                     /locus_tag="RO454_001520"
     CDS             14092..15336
                     /gene="ftsW"
                     /locus_tag="RO454_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDY0933368.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            15333..16400
                     /gene="murG"
                     /locus_tag="RO454_001521"
     CDS             15333..16400
                     /gene="murG"
                     /locus_tag="RO454_001521"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDY0933369.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            16454..17929
                     /gene="murC"
                     /locus_tag="RO454_001522"
     CDS             16454..17929
                     /gene="murC"
                     /locus_tag="RO454_001522"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDY0933370.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            17922..18842
                     /gene="ddlB"
                     /locus_tag="RO454_001523"
     CDS             17922..18842
                     /gene="ddlB"
                     /locus_tag="RO454_001523"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDY0933371.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            18844..19674
                     /gene="ftsQ"
                     /locus_tag="RO454_001524"
     CDS             18844..19674
                     /gene="ftsQ"
                     /locus_tag="RO454_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDY0933372.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            19671..20933
                     /gene="ftsA"
                     /locus_tag="RO454_001525"
     CDS             19671..20933
                     /gene="ftsA"
                     /locus_tag="RO454_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDY0933373.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            20994..22145
                     /gene="ftsZ"
                     /locus_tag="RO454_001526"
     CDS             20994..22145
                     /gene="ftsZ"
                     /locus_tag="RO454_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDY0933374.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            22246..23163
                     /gene="lpxC"
                     /locus_tag="RO454_001527"
     CDS             22246..23163
                     /gene="lpxC"
                     /locus_tag="RO454_001527"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDY0933375.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            23393..23905
                     /gene="secM"
                     /locus_tag="RO454_001528"
     CDS             23393..23905
                     /gene="secM"
                     /locus_tag="RO454_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDY0933376.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            23967..26672
                     /gene="secA"
                     /locus_tag="RO454_001529"
     CDS             23967..26672
                     /gene="secA"
                     /locus_tag="RO454_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDY0933377.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            26732..27130
                     /gene="mutT"
                     /locus_tag="RO454_001530"
     CDS             26732..27130
                     /gene="mutT"
                     /locus_tag="RO454_001530"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDY0933378.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(27218..27415)
                     /gene="yacG"
                     /locus_tag="RO454_001531"
     CDS             complement(27218..27415)
                     /gene="yacG"
                     /locus_tag="RO454_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDY0933379.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(27425..28168)
                     /gene="zapD"
                     /locus_tag="RO454_001532"
     CDS             complement(27425..28168)
                     /gene="zapD"
                     /locus_tag="RO454_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDY0933380.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            complement(28168..28788)
                     /gene="coaE"
                     /locus_tag="RO454_001533"
     CDS             complement(28168..28788)
                     /gene="coaE"
                     /locus_tag="RO454_001533"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDY0933381.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            28813..28857
                     /locus_tag="RO454_001534"
     CDS             28813..28857
                     /locus_tag="RO454_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933382.1"
                     /translation="MIIKTLKMSARKRN"
     gene            29013..30056
                     /gene="guaC"
                     /locus_tag="RO454_001535"
     CDS             29013..30056
                     /gene="guaC"
                     /locus_tag="RO454_001535"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDY0933383.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(30091..31293)
                     /gene="hofC"
                     /locus_tag="RO454_001536"
     CDS             complement(30091..31293)
                     /gene="hofC"
                     /locus_tag="RO454_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDY0933384.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(31283..32668)
                     /gene="gspE"
                     /locus_tag="RO454_001537"
                     /pseudo
     CDS             complement(31283..32668)
                     /gene="gspE"
                     /locus_tag="RO454_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            complement(32678..33118)
                     /gene="ppdD"
                     /locus_tag="RO454_001538"
     CDS             complement(32678..33118)
                     /gene="ppdD"
                     /locus_tag="RO454_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDY0933385.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            complement(33322..34215)
                     /gene="nadC"
                     /locus_tag="RO454_001539"
     CDS             complement(33322..34215)
                     /gene="nadC"
                     /locus_tag="RO454_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDY0933386.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            34303..34854
                     /gene="ampD"
                     /locus_tag="RO454_001540"
     CDS             34303..34854
                     /gene="ampD"
                     /locus_tag="RO454_001540"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDY0933387.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            34851..35705
                     /gene="ampE"
                     /locus_tag="RO454_001541"
     CDS             34851..35705
                     /gene="ampE"
                     /locus_tag="RO454_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDY0933388.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(35748..37118)
                     /gene="aroP"
                     /locus_tag="RO454_001542"
     CDS             complement(35748..37118)
                     /gene="aroP"
                     /locus_tag="RO454_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDY0933389.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
BASE COUNT         8863 a         9108 c        10431 g         9076 t
ORIGIN      
        1 tatttctctg cattccaata agggaaaggg agttaagtgt gacagtggag ttaagtatgc
       61 cagaggtaca aacagatcat ccagagacgg cggagttaag caagccacag ctacgcatgg
      121 tcgatctcaa cttattaacc gttttcgatg ccgtgatgca ggagcaaaac atcacccgtg
      181 ccgctcatgc tctgggaatg tcgcaacctg cggtcagtaa cgctgttgca cgcctgaagg
      241 tgatgtttaa tgacgagctt tttgttcgtt atggtcgtgg tattcaaccg actgctcgcg
      301 catttcaact ttttggctca gttcgtcagg cattgcaatt agtacaaaat gaattaccag
      361 gttcaggctt tgagcctgcc agtagtgaac gtgtatttca tctttgtgtc tgcagcccgt
      421 tagacagcat tctgacctcg cagatttata atcacattga gcagattgcg ccaaatatac
      481 atgttatgtt caagtcttca ttaaatcaga acactgaaca tcagctgcgt tatcaggaaa
      541 cggagtttgt gattagttat gaagacttcc atcgtcctga atttaccagc gtgccattat
      601 ttaaagatga aatggtgttg gtagccagca aaaatcatcc aacaattaaa ggcccgttac
      661 tgaaacatga tgtttataac gaacaacatg cggcggtttc gctcgatcgt ttcgcgtcat
      721 ttagtcaaac ctggtatgac acggtagata agcaagccag tatcgcgtat cagggcatgg
      781 caatgatgag cgtacttagc gtggtgtcgc aaacgcatct ggttgctatt gcgccgcgtt
      841 ggttggctga agagttcgct gaatccttag agttacaggt attaccgctg ccgttaaaac
      901 aaaacagcag aacctgttat ctctcctggc atgaagctgc cgggcgcgat aaaggccatc
      961 agtggatgga agagcaatta gtctcaattt gcaaacgcta actgaatgca gaataggtca
     1021 gacatcaatg tctggtttat tctgcatttt ttattgaatg tagaatttta ttctgaatat
     1081 ttgtgctctc tattttagga ttaattaaaa aaatagagaa attgctgtaa gttgtaggat
     1141 tcagccgatt tattatcaat ttcatcctct gtaatggagg attttatcgt ttcttttcac
     1201 ctttcctcct gtttattctt attaccccgt gtttatgtct ctgtctgcca attgcttaag
     1261 caagatcgga cggttaatgt gttttacaca ttttttccgt caaacagtga ggcaggccat
     1321 ggagatgttg tctggagccg agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca
     1381 agtattcggt tatcctggag gcgcggtcct tgatatttac gatgcattgc ataccgtggg
     1441 tggtatcgat catgttttag ttcgccatga gcaggcggcg gtgcatatgg ccgatggtct
     1501 ggcgcgcgcg accggggaag tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa
     1561 tgcgattact ggcattgcca ccgcttatat ggattccatt ccattagttg tcctttccgg
     1621 gcaggtagcg acttcgttga taggttatga tgcctttcag gagtgcgaca tggtggggat
     1681 ttctcgcccg gtggttaaac acagttttct ggttaagcaa acggaagaca ttccgcaggt
     1741 gctgaaaaag gctttctggc tagcggcaag tggtcgtcct ggacccgtgg tcgttgattt
     1801 accgaaagat attctaaatc cggcgaacaa attaccctat gtctggccgg agtcggtcag
     1861 tatgcgttct tacaatccca cgacctccgg gcataaaggg caaattaagc gcgctctgca
     1921 aacgctggtg gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa tcacggcggg
     1981 ctgccatctg cagttgaaag aagcggtgga ggcgttgaat ctgcccgttg tttcctcttt
     2041 gatggggctg ggggcgtttc cggcaacgca tcgtcaggca ctgggcatgt tgggaatgca
     2101 cggtacctac gaagccaata tgacgatgca taacgcggat gtgattttcg ccgtcggggc
     2161 acgttttgat gaccgaacga cgaacaatct ggcaaaatac tgcccaaatg ccactgttct
     2221 gcatatcgat attgatccaa cctcaatttc taaaaccgtg actgctgata tcccgattgt
     2281 gggggatgct cgccaggtcc tagaacaaat gcttgaactc ctgtcgcaag aatccgccca
     2341 tcaaccgctg gatgagatcc gcgactggtg gcagcagatt gaacagtggc gcgctcgtca
     2401 gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg caggcggtga tcgagactct
     2461 ttggcggttg actaatgggg acgcttacgt gacgtccgat gtcgggcagc accagatgtt
     2521 tgctgcgctt tattatccat tcgacaaacc gcgtcgctgg atcaactctg gcggcctcgg
     2581 cacgatgggt tttggtttac ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac
     2641 cgtggtttgc gtcactggtg acggcagtat tcagatgaac atccaggaac tgtctaccgc
     2701 cttgcaatac gagttgcccg tactggtggt gaatctcaat aatcgctatc tggggatggt
     2761 gaagcagtgg caggacatga tctattccgg ccgtcattca caatcttata tgcagtcctt
     2821 accagacttc gtccgtctgg cggaagccta tggtcacgtc gggatccaga tctctcatcc
     2881 gcaagaactg gaaagcaaac ttagcgaggc gctggaacag gtgcgcaata atcgcctggt
     2941 gtttgttgat gttaccgtcg atggcagtga gcacgtctac ccgatgcaga ttcgcggggg
     3001 cggaatggat gaaatgtggt taagcaaaac ggagagaacc tgattatgcg ccggatatta
     3061 tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct tttttcccag
     3121 cgtggctaca acattgaaag cctgaccgtt gcgccaaccg acgatccgac attatcgcgt
     3181 atgaccatcc agaccgtggg cgatgaaaaa gtgcttgagc aaatcgaaaa gcaattacac
     3241 aagctggtcg atgtcttgcg cgtgagcgag ttggggcagg gcgcgcacgt tgagcgggaa
     3301 atcatgctgg tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg
     3361 gaaatattcc gtgggcaaat tatcgatgtc acaccctcgc tttataccgt tcaattagca
     3421 ggcaccagcg ataagcttga tgcattttta gcatcgattc gcgatgtggc gaaaattgtg
     3481 gaagttgctc gctctggtgt ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa
     3541 tgatctcaat gcgcaatttg cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag
     3601 tgggaacctg gaataaaagc agttgccgca gttaattttc tgcgcttaga tgttaatgaa
     3661 tttaacccat accagtacaa tggctatggt ttttacattt tacgcaaggg gcaattgtga
     3721 aactggatga aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc tatgttatta
     3781 acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg
     3841 tgcgtgagca caattaccac ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac
     3901 gttctattgg tcttgtgatc cccgatctgg agaacaccag ctatacccgc atcgctaact
     3961 atcttgaacg ccaggcgcgg caacggggtt atcaactgct gattgcctgc tcggaagatc
     4021 agcccgacaa cgaaatgcgc tgtattgagc accttttaca gcgtcaggtt gatgccatta
     4081 ttgtttcgac gtcgttgcca cctgagcatc ctttttatca acgctgggct aacgacccgt
     4141 tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca cttcaccagc gtggttggtg
     4201 ccgatcagga tgatgccgaa atgctggcgg aagagttacg taagtttccc gccgagacgg
     4261 tgctttatct tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa caaggcttcc
     4321 gtactgcctg gaaagatgat ccgcgcgaag tgcatttcct ttatgccaac agctatgagc
     4381 gggaggcagc tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc
     4441 tgttcacaac gtcgtttgcg ttgttgcaag gggtgatgga tgtcacgcta cgtcgcgacg
     4501 gcaaactgcc ttctgacctg gcaattgcca cctttggcga taacgaactg ctcgacttct
     4561 tacagtgccc ggtgctggca gtggctcaac gtcaccgcga tgtcgcagaa cgtgtgctgg
     4621 agattgtcct ggcgagcctg gacgaaccgc gtaagccaaa acctggttta acgcgcatta
     4681 aacgtaatct ctatcgccgc ggcgtgctca gccgtagcta aaccgcgaac aaaagtacgc
     4741 gccaggtgaa tttctctctg gcgcgttgag tacgggaatg gacatcaata tccttaaagt
     4801 aaataagact attcctggct attattgata aatgctttta aacccgcccg ttaattaact
     4861 cacctgctga aatgcacaat aattaaatga tattgacagc gcgtttttgc attattttgt
     4921 tacatgcaga gatgaattgc cgatttaaca aacacttttc tttgtttttg cgcaaacctg
     4981 ctggcatcaa gcgccacaca gacgcaacag ggactgttaa ccagagaaga tatgtcctaa
     5041 aatgccgctc gcgtcgcaaa ctgacacttt atatttgctg tggaaaatag tgagtcattt
     5101 taaaacggtg atgacgatga gggatttttt cttacagcta ttcataacgt taatttgctt
     5161 cgcacgttgg acgtaaaata aacaacgctg atattagccg taaacatcgg gttttttacc
     5221 tcggtatgcc ttgtgactgg cttgacaagc ttttcctccg ctccgtaaac tccttttagt
     5281 gggaaattgt ggggcaaagt gggaataagg ggtgaggctg gcatgttccg gggagcaacg
     5341 ttagtcaatc tcgacagcaa agggcgctta tcagtgccta cccgttatcg ggaacagctg
     5401 cttgagaacg ctgccggtca aatggtttgc accattgaca ttcatcaccc gtgcctgctg
     5461 ctttaccccc tgcctgaatg ggaaattatc gagcaaaaat tatcgcgtct gtcgagcatg
     5521 aacccggttg agcgccgtgt gcagcgccta ctgttaggtc atgccagcga atgtcagatg
     5581 gatggcgcag gtcgattgtt aatcgcgcca gtactgcggc aacatgccgg gctgacaaaa
     5641 gaagtgatgc tggttggaca gttcaacaag tttgagctgt gggatgaaac aacctggcat
     5701 caacaggtca aggaagatat cgacgcagag cagttggcta ccggagactt atcggagcga
     5761 ctgcaggact tgtctctata aaatgatgga aaactataaa catactacgg tgctgctgga
     5821 tgaagccgtt aatggcctca atatccgtcc tgatggcatc tacattgatg ggacttttgg
     5881 tcgcggtggt cactcacgtc tgatcctctc gcagcttggc gaagaggggc gtttgctggc
     5941 gatcgatcgc gacccgcagg ctatcgccgt tgcgaagact attgatgatc cgcgcttctc
     6001 catcatccac ggacctttct ctgcgctggg cgaatatgtt gccgagcgcg atcttatcgg
     6061 caagatcgac ggcattctcc tcgatcttgg cgtctcttca ccgcaacttg atgatgctga
     6121 acgcggcttt tcctttatgc gcgatggtcc gctggacatg cgtatggacc caacccgtgg
     6181 gcagtcagcc gctgaatggc tacaaaccgc agaagaagcc gatatcgcct gggtattgaa
     6241 aacctatggt gaagagcgtt ttgccaaacg cattgctcgc gccattgtcg agcgtaaccg
     6301 cgaacagccg atgacccgca ccaaagaact ggcggaagtc gtggctgccg caacgccggt
     6361 gaaagataag tttaaacatc ccgcgacccg taccttccag gcggtgcgca tttgggtaaa
     6421 cagtgaactg gaggagatag agcaggcgct aaaaagctcg ctcaatgtgc tggccccggg
     6481 ggggcggctt tcgatcatca gcttccactc acttgaagac cgcattgtga aacgttttat
     6541 gcgtgaaaac agccgcggtc cgcaagttcc ggcagggtta ccgatgacag aagagcagct
     6601 caaaaaactg ggggggcgtc agttgcgagc actaggtaag ttgatgccgg gcgaagaaga
     6661 ggtggctgag aaccctcgtg cccgtagttc agttctgcgt attgcagaga ggacgaacgc
     6721 atgatcagca gagtgacaga agctctaagc aaagttaaag gatcgatggg aagccacgag
     6781 cgccatgcct tgcctggcgt tatcggtgac gatcttttgc gatttgggaa gctgccactc
     6841 tgcctgttca tttgcattat tttgacggcg gtgaccgtgg taaccacggc gcaccatacc
     6901 cgtttactga ccgctcagcg cgaacaactg gtgctggagc gagatgcttt agacattgaa
     6961 tggcgcaacc tgatccttga agagaatgcg ctcggcgacc atagccgggt ggaaaggata
     7021 gccacggaaa agctgcaaat gcagcatgtt gatccgtcac aagaaaatat cgtagtgcaa
     7081 aaataaggat aaacgcgacg catgaaagca gcggcgaaaa cgcagaaacc aaaacgtcag
     7141 gaagaacatg ccaactttat cagttggcgt tttgcgttgt tatgcggctg tattctcctg
     7201 gcgctggctt ttctgctcgg acgcgtagcg tggttacaag ttatctcccc ggatatgctg
     7261 gtgaaagagg gcgacatgcg ttctcttcgc gttcagcaag tttccacctc ccgcggcatg
     7321 attactgacc gttctggtcg cccgttagcg gtgagtgtgc cagtaaaagc gatttgggct
     7381 gacccgaaag aagtgcatga cgctggcggt atcagcgtcg gtgaccgctg gaaggcgctg
     7441 gctaacgcgc tcaatattcc gctggatcag ctctcagccc gcattaacgc caacccgaaa
     7501 gggcgcttta tttatctggc gcgtcaggta aaccctgaca tggcggacta catcaaaaaa
     7561 ctgaaactgc cgggaattca tctgcgtgaa gaatctcgcc gttattatcc atccggcgaa
     7621 gtgactgctc acctcatcgg ctttactaac gtcgatagcc aagggattga gggggtcgag
     7681 aagagtttcg ataaatggct taccgggcag ccgggtgagc gcattgtgcg taaagaccgc
     7741 tatggtcgcg taattgaaga tatttcttct actgacagcc aggcagcgca caacctgacg
     7801 ctgagtattg atgaacgcct gcaggcgctg gtttatcgcg aactgaacaa cgcagtggcc
     7861 tttaacaagg ctgaatctgg tagcgccgtg ctggtggatg tcaacaccgg tgaagtgctg
     7921 gcgatggcta acagcccgtc atacaaccca aataatctga gcggcacgcc gaaagaagcg
     7981 atgcgtaacc gtaccatcac cgacgtgttt gaaccgggct caacggttaa accgatggtg
     8041 gtgatgaccg cgttgcaacg tggcgtggtg cgggaaaact cggtactcaa taccgttcct
     8101 tatcgaatta acggccacga aatcaaagac gtggcacgct acagcgaatt aaccctgacc
     8161 ggggtattac agaagtcgag taacgtcggt gtttccaaac tggcgttagc gatgccgtcc
     8221 tcagcgttag tagatactta ctcacgtttt gggctgggaa aagcgaccaa tttggggttg
     8281 gtcggagaac gcagtggctt atatcctcaa aaacaacggt ggtctgacat agagagggcc
     8341 accttctctt tcggctacgg gctaatggta acaccgttac agttagcgcg agtctacgca
     8401 actatcggca gctacggcat ttatcgccca ctgtcgatta ccaaagttga ccccccggtt
     8461 cccggtgaac gtgtcttccc ggaatccatt gttcgcaccg tggtgcatat gatggaaagc
     8521 gtggcgctac cgggcggcgg cggcgtgaag gcggcgatta aaggctatcg tatcgccatt
     8581 aaaaccggta ccgcgaaaaa ggtcgggccg gatggtcgct acatcaataa atatattgct
     8641 tataccgcag gcgttgcgcc tgcgagtcag ccgcgcttcg cgctggttgt tgttatcaac
     8701 gatccgcagg cgggtaaata ctacggcggc gccgtttccg cgccggtctt tggtgccatc
     8761 atgggcggcg tattgcgtac catgaacatc gagccggatg cgctgacaac aggcgataaa
     8821 aatgaatttg tgattaatca aggcgagggt acaggtggca gatcgtaatt tgcgcgacct
     8881 tcttgctccg tgggtgccag acgcaccttc gcgagcactg cgagagatga cactcgacag
     8941 ccgtgtggct gcggcgggcg atctctttgt agctgtagta ggtcatcagg cggacgggcg
     9001 tcgatatatc ccgcaggcga tagcgcaagg tgtggctgcc attattgcag aggcgaaaga
     9061 tgaggcgacc gacggtgaaa tccgtgaaat gcacggcgta ccggtcatct atctcagcca
     9121 gctcaacgag cgtttatctg cactggcggg ccgcttttac catgaaccct ctgacaattt
     9181 acgtctcgtg ggcgtaacgg gcaccaacgg caaaaccacg actacccagc tgttggcgca
     9241 gtggagtcaa ctgcttggcg aaaccagcgc ggtaatgggc accgttggta acggcctgct
     9301 ggggaaagtg atcccgacag aaaatacaac cggttcggca gtcgatgttc agcatgagct
     9361 ggcggggctg gtggatcagg gcgcgacttt ttgcgcaatg gaagtttcat cccacgggct
     9421 ggttcaacac cgtgtggcgg cattgaaatt tgcggcgtcg gtctttacca acttaagccg
     9481 cgatcacctt gattatcatg gtgatatgga acactacgaa gccgcgaaat ggctgcttta
     9541 ttctgcacat cattgcggtc aggcgattat taacgccgac gatgaagtgg gccgccgctg
     9601 gctggcaaaa ctgccggacg cggttgcggt atcaatggaa gatcatatca atccgaactg
     9661 tcacggacgc tggttgaaag cgaccgacgt gaactatcac gacagcggtg cgaccatttg
     9721 ctttagctcg agttggggcg atggggaaat tgaaagtcgc ctgatgggcg cattcaacgt
     9781 cagcaacctg ctgctggcgc tggcgacact gctggcactc ggctatccgc tggctgattt
     9841 gctgaaaacc gccgcgcgtc tgcaaccggt ttgcggacgt atggaagtgt tcactgcacc
     9901 aggcaaaccg acggtggtgg tggattacgc gcatacgccg gatgcactgg aaaaagcctt
     9961 acaggcggcg cgcctgcact gtgcgggcaa gctgtggtgc gtctttggct gcggtggcga
    10021 tcgtgataaa ggcaaacgtc cactgatggg cgcaattgct gaagagtttg ctgacgtggt
    10081 ggtggtgacg gacgataacc cgcgtaccga agagcctcgt gccatcatca acgatattct
    10141 ggcgggaatg ttagatgccg gatatgccaa agtgatggaa ggccgtgctg aagcggtgac
    10201 ttgcgccgtt atgcaggcta aagagaatga tgtggtactg gtcgcgggca aaggccatga
    10261 ggactaccag attgttggca atcagcgtct ggactactcc gatcgcgtca cggtggcgcg
    10321 tctgctgggg gtgattgcat gattagcgta acccttagcc aacttaccga tattctcaac
    10381 ggtgaactgc aaggtgcaga tattaccctt gatgctgtaa ccactgacac gcgaaaactg
    10441 acgccgggct gcctgtttgt tgccctgaaa ggcgaacgtt tcgatgctca tgattttgcc
    10501 gaccaggcga aagctggcgg cgcaggcgca ctactggtta gccgtccgct ggatattgat
    10561 ctgccgcagt taatcgtcaa ggatacgcgt ctggcgtttg gtgaactggc tgcatgggtt
    10621 cgccagcaag ttccggcgcg cgtggttgct ctgacaggtt cctccggcaa aacctccgtt
    10681 aaagagatga cggcggcaat tttaagccag tgcggcaaca cactttatac ggcaggcaat
    10741 ctcaataacg acatcggtgt accgatgacg ctgttgcgct taacgccgga atacgattac
    10801 gcagttattg aacttggcgc gaaccatcag ggtgaaattg cctggactgt gagtctgact
    10861 cgcccggaag cggcgctggt caacaacctg gcagcggcgc atctggaagg ttttggctcg
    10921 cttgcgggtg tcgcgaaagc gaaaggtgaa atctttagcg gcctgccgga aaacggtatc
    10981 gccattatga acgccgacaa caacgactgg ttgaactggc agagcgtaat tggctcacgc
    11041 aaagtgtggc gtttctcacc taatgccgcc aacagcgatt tcaccgccac caatatccat
    11101 gtaacctcgc acggtacgga atttaccctg caaaccccta caggtagcgt cgatgttctg
    11161 ctgccgttgc cggggcgtca caatattgcg aatgcgctgg cagccgctgc gctctccatg
    11221 tctgtgggcg caacgcttga tgctatcaaa gcggggctgg taaatctgaa agctgttcca
    11281 ggccgtctgt tccccataaa actggcagaa aaccagttgc tgctcgacga ctcctacaac
    11341 gccaatgtcg gttcaatgac cgccgctgtt caggttctgg ctgaaatgcc gggctaccgc
    11401 gtgctggtgg tgggcgatat ggcggaactg ggcgctgaaa gcgaagcctg ccatgtacag
    11461 gtgggcgaag cggcaaaagc tgctggtatt gactgcgtgt taagtgtggg taaacaaagc
    11521 catgctatca gcaccgccag cggcgttggc gaacattttt ccgataaaac tgcgcttatc
    11581 gcgcgtctta aatcactgat tgctgagcaa caggtaatta cgattttagt taagggttca
    11641 cgtagtgctg ccatggaaga ggtagtacgc gctttacagg agaatgggac atgttagttt
    11701 ggctggccga acatttggtc aaatattatt ccggctttaa cgtcttttcc tatctgacgt
    11761 ttcgcgccat cgtcagcctg ctgaccgcgc tgttcatctc attgtggatg ggcccgcgta
    11821 tgattgctca tttgcaaaaa ctttcctttg gtcaggtggt gcgtaacgac ggtcctgaat
    11881 cacacttcag caagcgcggt acaccgacca tgggcgggat aatgatcctg acggcgattg
    11941 tgatctccgt gctgctgtgg gcttacccgt ccaatccgta cgtctggtgc gtactggtgg
    12001 tgctggtagg ttacggtgtt attggctttg ttgatgatta tcgcaaagtg gtgcgtaaag
    12061 acaccaaggg cctgattgcc cgttggaagt atttctggat gtcggtcatt gcactgggtg
    12121 ttgccttcgc gctgtacctt gccggcaaag acacgcccgc aacgcagttg gtggttccgt
    12181 tctttaaaga tgtgatgccg caactggggc tgttctacat tctgctggct tactttgtca
    12241 ttgtgggtac tggcaacgcg gtaaacctga ccgatggtct cgacggtctg gcaattatgc
    12301 cgaccgtatt tgtcgccggt ggttttgcgc tggtggcgtg ggcgaccggc aacatgaact
    12361 ttgccagcta tttacacata ccgtatctac gacacgccgg ggaactggtc attgtctgta
    12421 ccgcgatagt cggggcaggg ctgggcttcc tgtggtttaa cacctatccg gcgcaggtct
    12481 ttatgggcga tgtaggttcg ctggcgttag gtggtgcgtt aggtattatc gccgtactgc
    12541 tgcgtcagga attcctgctg gtgattatgg ggggcgtgtt cgtggtagaa acgctttccg
    12601 tcatcctgca ggtcggctcc tttaaactgc gcggacaacg tattttccgc atggcaccga
    12661 ttcatcacca ctatgaactg aaaggctggc cggaaccgcg cgtcattgtg cgtttctgga
    12721 ttatttcgct gatgctggtc ctgattggtc tggcaacgct gaaggtacgt taatcatggc
    12781 tgattaccag ggtaaaaatg tcgtcattat cggcctgggc ctcaccgggc tttcctgcgt
    12841 ggactttttc ctcgctcgcg gtgtgacgcc gcgcgttatg gatacgcgta tgacaccgcc
    12901 tggcctggat aaattacccg aagccgtaga acgccacacg ggcggtctga atgatgaatg
    12961 gctgatggcg gcagatctga ttgtcgccag tcccggtatt gcactggcgc atccatcctt
    13021 aagcgctgcc gctgatgccg gaatcgaaat cgttggcgat atcgagctgt tctgtcgtga
    13081 agcacaagca ccgattgtgg cgatcaccgg ttctaacggc aaaagcacgg tcaccacgct
    13141 agtgggtgaa atggcgaaag cggcgggggt taacgttggt gtgggtggta atattggcct
    13201 gcctgcgttg atgctgttgg atgatgagtg cgaactgtac gtgctggaac tgtcgagttt
    13261 ccagctggaa accacttcca gcttacaagc ggtggcggcg accattctga acgtgactga
    13321 agatcatatg gatcgctatc cgtttggttt acaacagtat cgtgcagcaa aactgcgcat
    13381 ttacgaaaac gcaaaagttt gcgtggttaa tgctgatgat gccttaacaa tgccgattcg
    13441 cggtgcggat gaacgctgcg tcagctttgg cgtcaacatg ggcgactatc acctgaatca
    13501 tcagcagggc gaaacctggc tgcgggtgaa aggcgagaaa gtgctgaacg tgaaagaaat
    13561 gaaactttcc gggcagcata actacaccaa tgcgctggcg gcgctggcgc tggcagatgc
    13621 cgcagggtta ccgcgtgcca gcagcctgaa agcgttaacc acattcactg gtctgccgca
    13681 tcgctttgaa gttgtgctgg agcataacgg tgtgcgctgg gttaacgatt caaaagcgac
    13741 caacgtcggc agtacggaag cggcgctgaa tggcctgcat gtagacggca cgctgcattt
    13801 gttgctgggt ggcgatggta aatcggcgga ctttagccca ctggcgcgtt acctgaatgg
    13861 cgataatgta cgtctgtatt gtttcggtcg ggacggcgcg cagctggcgg cgctacgccc
    13921 ggaagtggca gaacaaaccg aaaccatgga acaggcgatg cgcttgcttg ctccgcgtgt
    13981 tcagccgggc gatatggttc tgctctcccc ggcctgtgcc agccttgatc aattcaagaa
    14041 ctttgaacaa cgaggcaatg agtttgcccg tctggcgaag gagttaggtt gatgcgttta
    14101 tctctccctc gcctgaaaat gccgcgcctg ccaggattca gtattctggt ctggatctcc
    14161 acggcgctaa agggctgggt gatgggctcg cgggaaaaag ataccgacag cctgatcatg
    14221 tacgatcgca ccttactgtg gctgaccttc ggcctcgcgg cgattggctt tatcatggtg
    14281 acctcggcgt caatgcccat agggcaacgc ttaaccaacg atccgttctt cttcgcgaag
    14341 cgtgatggcg tctatctgat cctggcattc attctggcga tcattacgct gcgtctgccg
    14401 atggagttct ggcaacgcta cagtgccacg atgctgcttg gatctatcat cctgctgatg
    14461 atcgttctgg tagtgggtag ctcggttaaa ggggcatcgc gttggatcga tctcggtttg
    14521 ctgcgtatcc agcctgcgga gctgacaaaa ctgtcgctgt tttgctatat cgccaactat
    14581 ctggtgcgta aaggcgacga agtacgtaat aacctgcgcg gctttctgaa accgatgggc
    14641 gtaattctgg tgttggctgt gttactgctg gcacagccag accttggtac ggtggtggtg
    14701 ttgtttgtga ctacgctggc gatgttgttc ctggcgggag cgaaattgtg gcagttcatt
    14761 gccattatcg gtatgggcat ttcagcggtt gtgttgctga tcctcgccga accgtaccgt
    14821 atccgccgtg ttaccgcatt ctggaatccg tgggaagatc cctttggcag cggctatcag
    14881 ttaacgcaat cgttgatggc gtttggtcgc ggtgaactgt ggggacaagg tttaggtaac
    14941 tcggtacaaa aactggagta tctgccggaa gcgcacactg actttatttt cgccattatc
    15001 ggcgaagaac tgggatatgt cggtgtggtg ctggcacttt taatggtatt cttcgtcgct
    15061 tttcgcgcga tgtcgattgg ccgcaaagca ttagaaattg accaccgttt ttccggtttt
    15121 ctcgcctgtt ctattggtat ctggtttagt tttcaggcgc tggttaacgt aggcgcggcg
    15181 gcggggatgt taccgaccaa aggtctgaca ttgccgctga tcagttacgg tggttcgagc
    15241 ttactgatta tgtcgacagc catcatgatg ctgttgcgta ttgattatga aacgcgtctg
    15301 gagaaagcgc aggcgtttgt acgaggttca cgatgagtgg tcaaggaaag cgattaatgg
    15361 tgatggcagg cggaaccggt ggacatgtat tcccggggct ggcggttgcg caccatctaa
    15421 tggctcaggg ttggcaagtt cgctggctgg ggactgccga ccgtatggaa gcggacttag
    15481 tgccaaaaca tggcatcgaa attgatttca ttcgtatctc tggtctgcgt ggaaaaggta
    15541 taaaagcact gatagctgcg ccgctgcgta tcttcaacgc ctggcgtcag gcgcgggcga
    15601 ttatgaaagc gtacaaacct gacgtggtgc tcggtatggg cggctatgta tcaggtccag
    15661 gtggtctggc cgcgtggtcg ttaggcattc cggttgtact tcatgaacaa aacggtattg
    15721 cgggcttaac caataaatgg ctggcgaaga ttgctaccaa agtgatgcag gcgtttccag
    15781 gtgctttccc taatgcggaa gtggtgggta acccggtgcg taccgatgtg ctggcgctgc
    15841 cgttgccgca gcaacgtttg gctggacgtg aaggtccggt tcgtgtgttg gtagtgggtg
    15901 gttcccaggg cgcacgcatt cttaatcaga caatgccgca ggttgctgcg aaactgggtg
    15961 attcagtcac tatctggcat cagagcggca aaggttcgca acaatccgtt gaacaggcgt
    16021 atgccgaagt ggggcaaccg cagcataaag tgacggaatt tattgatgat atggcggcgg
    16081 cgtatgcgtg ggcggatgtc gtcgtttgcc gctccggtgc gttaacggtg agtgaaatcg
    16141 ccgcggcagg actaccggcg ttgtttgtgc cgtttcaaca taaagaccgt cagcaatact
    16201 ggaatgcgct accgctggaa aaagcgggcg cagccaaaat tatcgagcag tcacagctta
    16261 gcgtggatgc tgtcgcgaac accctggccg ggtggtcgcg agaaacctta ttaaccatgg
    16321 cagaacgcgc ccgcgctgca tccattccgg atgccaccga gcgagtggca aatgaagtga
    16381 gccgggctgc ccgggcgtaa ttgtagcgat gccttttgca tcgtatgaat ttaagaagtt
    16441 aatggcgtaa agaatgaata cacaacaatt ggcaaaactg cgttccatcg tgcccgaaat
    16501 gcgtcgcgtt cggcacatac attttgtcgg catcggtggt gccggtatgg gcggtattgc
    16561 cgaagttctg gccaatgaag gttatcagat cagtggttcc gatttagcgc caaatccggt
    16621 cacgcagcag ttaatgaatc tgggagcgac gatttatttc aaccatcgcc cggaaaacgt
    16681 acgtgatgcc agcgtggtcg ttgtttccag cgcgatttct gccgataacc cggaaattgt
    16741 cgcagctcat gaagcgcgta ttccggtgat ccgtcgtgcc gaaatgctgg ctgagttaat
    16801 gcgttttcgt catggcatcg ccattgccgg aacgcacggc aaaacgacaa ccaccgcgat
    16861 ggtttccagc atctacgcag aagcggggct cgacccaacc ttcgttaacg gcgggctggt
    16921 aaaagcggcg ggggttcatg cgcgtttggg gcatggtcgg tacctgattg ccgaagcaga
    16981 tgagagtgat gcatcgttcc tgcatctgca accgatggtg gcgattgtca ccaatatcga
    17041 agccgaccac atggatacct accagggcga ctttgagaat ttaaaacaga cttttattaa
    17101 ttttctgcac aacctgccgt tttacggtcg cgcggtgatg tgtgttgatg atccggtgat
    17161 ccgcgaattg ttaccgcgtg tggggcgtca gaccacgact tacggcttca gcgaagatgc
    17221 cgacgtgcgt gtagaagatt atcagcagat tggcccgcag gggcacttta cgctgctgcg
    17281 ccaggacaaa gagccgatgc gcgtcaccct gaatgcgcca ggtcgtcata acgcgctgaa
    17341 cgccgcagct gcggttgcgg ttgctacgga agagggaatt gacgacgagg ctattttgcg
    17401 tgcgctggag agcttccagg ggacggggcg ccgtttcgat ttcctcggtg aattcccgct
    17461 ggagccagtg aatggtaaaa gcggtacggc aatgctggtc gatgactacg gccaccaccc
    17521 gacggaagtg gacgccacta ttaaagcggc gcgtgcaggc tggccggata aaaacctggt
    17581 aatgctgttt cagccgcacc gttttacccg tacgcgcgac ctgtatgatg atttcgccaa
    17641 tgtgctgacg caggttgata ccctgttgat gctggaagtg tatccggctg gcgaagcgcc
    17701 aattccggga gcggacagcc gttcgctgtg tcgcacaatt cgtggacgtg ggaaaattga
    17761 tcccattctg gtgccggacc cggcgcaggt agccgagatg ctggcaccgg tattaaccgg
    17821 taacgacctg attctcgttc agggggctgg taatatcgga aaaattgctc gttctttagc
    17881 tgaaatcaaa ctgaagccgc aaactccgga ggaagaacaa catgactgat aaaatcgcgg
    17941 tcctgttggg cgggacctcc gctgagcggg aagtttctct gaattctggc gcagcggtgt
    18001 tagccgggct gcgtgaaggc ggtattgacg cgtatcctgt cgacccgaaa gaagtcgacg
    18061 tgacgcaact gaagtcgatg ggttttcaga aagtgtttat cgcgctacac ggtcgcggcg
    18121 gtgaagatgg tacgctgcag ggcatgctcg agctgatggg cttgccttat accggcagcg
    18181 gagtgatggc atctgcgctt tcaatggata aactacgcag caaacttctg tggcagagtg
    18241 ccggtttacc ggtcgcgccg tgggtagcat taacccgcgc agagtttgaa aaaggcctta
    18301 acgataagca gttagcagaa atttcttctc tgggtttgcc ggtaatcgtt aagccgagcc
    18361 gcgaaggttc cagtgtggga atgtcaaaag tagtagcaga aaatgctcta caagatgcat
    18421 taagattggc atttcagcac gatgaagaag tattgattga aaaatggcta agtgggccgg
    18481 agttcacggt tgcgatactc ggtgaagaaa ttttaccgtc aatacgtatt caaccggccg
    18541 gaaccttcta tgattatgag gcgaagtatc tctctgatga gacacagtat ttctgccccg
    18601 caggtctgga agcgtcacaa gaggccaatt tgcaggcatt agtgctaaaa gcatggacga
    18661 cgttaggttg caaaggatgg ggacgtattg acgttatgct ggacagcgat ggacagtttt
    18721 atctgctgga agccaatacc tcaccgggta tgaccagcca cagtctggtg ccgatggcgg
    18781 cacgtcaggc aggtatgagc ttctcgcagt tggtagtacg aattctggaa ctggcggact
    18841 aatatgtcgc aggctgctct gaacacgcga aatagcgaag aagaggtttc ttctcgccgc
    18901 aataatggaa cgcgtctggc ggggatcctt ttcctgctga ccgttttaac gacagtgttg
    18961 gtgagcggct gggtcgtgtt gggctggatg gaagatgcgc aacgcctgcc gctctcaaag
    19021 ctggtgttga ccggtgaacg ccattacacg cgtaatgacg atatccggca gtcgatcctg
    19081 gcattgggtg agccaggcac ctttatgacc caggatgtca acatcatcca gacgcaaata
    19141 gaacaacgcc tgccgtggat taagcaggtg agcgtcagaa agcagtggcc tgatgaattg
    19201 aagattcatc tggttgaata tgtgccgatt gcgcggtgga atgatcaaca tatggtagac
    19261 gcggaaggaa acaccttcag cgtgccgcca gatcgcacca gcaagcaggt gcttccaatg
    19321 ctgtatggcc cggaaggcag cgccaatgaa gtgttgcagg gctatcgcga aatggggcag
    19381 atgctggcaa aggacagatt tactctgaag gaagcggcga tgaccgcgcg acgttcctgg
    19441 cagttgacgc tgaataacga tattaagctc aatcttggcc ggggcgatac gatgaaacgt
    19501 ttggctcgct ttgtagaact ttatccggtt ttacagcagc aggcgcaaac cgatggcaaa
    19561 cggattagct acgttgattt gcgttatgac tctggagcgg cagtaggctg ggcgcccttg
    19621 ccgccagagg aatctactca gcaacaaaat caggcacagg cagaacaaca atgatcaagg
    19681 cgacggacag aaaactggta gtaggactgg agattggtac cgcgaaggtt gccgctttag
    19741 taggggaagt tctgcccgac ggtatggtca atatcattgg cgtgggtagt tgcccgtcgc
    19801 gtggtatgga taaaggcggg gtgaacgacc tcgaatccgt ggtcaagtgc gtacaacgcg
    19861 ccattgacca ggcagaattg atggcagatt gtcagatatc ttcggtatat ctggcgcttt
    19921 ctggtaagca catcagctgc cagaatgaaa ttggtatggt gcctatttct gaagaagaag
    19981 tgacgcaaga agatgtggaa aacgtcgtcc ataccgcgaa atcggtgcgt gtgcgcgatg
    20041 agcatcgtgt gctgcatgtg atcccgcaag agtatgcgat tgactatcag gaagggatca
    20101 agaatccggt aggactttcg ggcgtgcgga tgcaggcaaa agtgcacctg atcacatgtc
    20161 acaatgatat ggcgaaaaac atcgtcaaag cggttgaacg ttgtgggctg aaagttgacc
    20221 aactgatatt tgccggtctg gcatcaagtt attcggtatt gacggaagat gaacgtgaac
    20281 tgggtgtctg cgtcgtcgat atcggtggtg gtacaatgga tatcgccgtt tataccggtg
    20341 gggcattgcg ccacacgaag gtaattcctt atgctggcaa tgtcgtgacc agtgatatcg
    20401 cttacgcctt tggcacaccg ccaagtgacg ccgaagcgat taaagttcgc cacggttgtg
    20461 cgctgggctc catcgttgga aaagatgaga gcgtggaagt gccgagcgta ggcggtcgtc
    20521 cgccgcggag tctgcaacgt cagaccctgg cagaggtgat cgagccacgc tataccgagc
    20581 tgctcaatct ggtcaacgaa gagatattgc agttgcagga aaagcttcgc caacaagggg
    20641 ttaaacatca cctggcggca ggcattgtat taaccggtgg cgcagcgcag atcgaaggtc
    20701 ttgcagcctg tgctcagcgc gtgtttcata cgcaagtgcg tatcggcgcg ccgctgaaca
    20761 ttaccggttt aacggattat gctcaggagc cgtattattc gacggcggtg ggattgcttc
    20821 actatgggaa agagtcacat cttaacggtg aagctgaagt agaaaaacgt gttacagcat
    20881 cagttggctc gtggatcaag cgactcaata gttggctgcg aaaagagttt taatttttat
    20941 gaggccgacg atgattacgg cctcaggcga caggcacaaa tcggagagaa actatgtttg
    21001 aaccaatgga acttaccaat gacgcggtga ttaaagtcat cggcgtcggc ggcggcggcg
    21061 gtaatgctgt tgaacacatg gtgcgtgagc gcattgaagg tgttgaattc ttcgcggtaa
    21121 ataccgatgc acaagcgctg cgtaaaacag cggttggaca gacgattcaa atcggtagcg
    21181 gtatcaccaa aggactgggc gctggcgcta atccagaagt tggccgcaat gcggctgatg
    21241 aggatcgcga tgcactgcgt gcggcgctgg aaggtgcaga catggtcttt attgctgcgg
    21301 gtatgggtgg tggcaccggt acaggtgcag caccagtcgt cgctgaagtg gcaaaagatt
    21361 tgggtatcct gaccgttgct gtcgtcacta agcctttcaa ctttgaaggc aagaagcgta
    21421 tggcattcgc ggagcagggg atcactgaac tgtccaagca tgtggactct ctgatcacta
    21481 tcccgaacga caaactgctg aaagttctgg gccgtggtat ctccctgctg gatgcgtttg
    21541 gcgcagcgaa cgatgtactg aaaggcgctg tgcaaggtat cgccgaactg attactcgtc
    21601 cgggtttgat gaacgtggac tttgcagacg tacgcaccgt aatgtctgaa atgggctacg
    21661 caatgatggg ttctggtgtg gcgagcggtg aagaccgtgc ggaagaagct gctgaaatgg
    21721 ctatctcttc tccgctgctg gaagatatcg acctgtctgg cgcacgcggc gtgctggtaa
    21781 acatcacagc gggcttcgac ctgcgtctgg atgagttcga aacggtaggt aacaccatcc
    21841 gtgcatttgc ttccgacaac gcgaccgtgg ttatcggtac ttctcttgac ccggatatga
    21901 atgacgagct gcgcgtaact gttgttgcga caggtatcgg catggacaaa cgtcctgaaa
    21961 tcactctggt gaccaataag caggttcagc agccagtgat ggatcgctac cagcagcacg
    22021 gtatggctcc gctgacccag gagcagaagc cggttgctaa agtcgtgaat gacaatgcgc
    22081 cgcaaactgc gaaagagccg gattatctgg atatcccagc attcctgcgt aagcaagctg
    22141 attaagaatt gactggaatt tgggtttcga ggctctttgt gctaaactgg cccgccgaat
    22201 gtatagtaca cttcggttgg ataggtaatt tggcgagata atacgatgat caaacaaagg
    22261 acacttaaac gtatcgttca ggcgacgggt gtcggtttac ataccggcaa gaaagtcacc
    22321 ctgacgttac gccctgcgcc ggccaacacc ggggtcatct atcgtcgcac cgacttgaat
    22381 ccaccggtag atttcccggc cgatgccaaa tctgtgcgtg ataccatgct ctgtacgtgt
    22441 ctggtcaacg agcatgatgt acggatttca accgtagagc acctcaatgc tgctctcgcg
    22501 ggcttgggca tcgataacat tgttatcgaa gttaacgcgc cggaaatccc gatcatggac
    22561 ggcagcgccg ctccgtttgt atacctgctg cttgacgccg gtatcgacga gttgaactgc
    22621 gccaagaaat ttgttcgcat taaagagact gttcgtgtcg aagatggcga taagtgggct
    22681 gaatttaaac cgtacaatgg tttttcgctg gatttcacca tcgattttaa ccatccggct
    22741 attgattcca gcaaccagcg ctatgcgatg aacttctccg ctgatgcgtt tatgcgccag
    22801 atcagccgtg cgcgtacgtt cggtttcatg cgtgatatcg aatatctgca gtcccgtggt
    22861 ttgtgcctgg gcggcagctt cgattgtgcc atcgttgttg acgattatcg cgtactgaac
    22921 gaagacggcc tgcgttttga agacgaattt gtgcgtcaca aaatgctcga tgcgatcggt
    22981 gacttgttca tgtgtggtca caatattatt ggtgcattta ccgcttataa atccggtcat
    23041 gcactgaata acaaactgct gcaggctgtc ctggcgaaac aggaagcctg ggaatatgtg
    23101 accttccagg acgacgcaga actgccgttg gccttcaaag cgccttcagc cgtactggca
    23161 taacgacatt tatactgtcg tataaaattc gactggcaaa tctggcactc tctccggcca
    23221 ggtgaaccag tcgttttttt tgaattttat aagagctata aaaaacggtg cgaacgctgt
    23281 tttcttaagc acttttacgc acaacttatc ttcattcgtg ctgtggactt caggctttaa
    23341 tgataagatt tgtgcgctaa atacgtttga atacgatcgg gatggcaata acgtgagtgg
    23401 aatactgacg cgctggcgac agtttggtaa acgctacttc tggccgcatc tcttattagg
    23461 gatggtcgcg gcgagtttag gtttgcctgc actcagcaat gccgccgaac caaacgcgcc
    23521 cgcaaaagcg acaacccgca accacgagcc ttcagccaaa gttaactttg gtcaattggc
    23581 cttgctggaa acgaacacgc gccgcccgaa ttcgaactat tccgttgatt actggcatca
    23641 acatgccatt cgcacggtaa tccgccatct ttcctttgca atggcaccgc aaacgctgcc
    23701 cgttgctgaa gaatctttgc ctcttcaggc gcaacatctt gcattactgg atacgctcag
    23761 cgcgctgctg acccaggaag gcacgccgtc tgaaaagggt tatcgcattg attatgcgca
    23821 ttttacccca caagcaaaat tcagcacgcc cgtctggata agccaggcgc aaggcatccg
    23881 tgctggccct caacgcctca gctaacaaca ataaaccttt acttcatttt attaactccg
    23941 caacgcgggg cgtttgagat tttattatgc taatcaaatt attaactaaa gttttcggta
    24001 gtcgtaacga tcgcaccctg cgccggatgc gcaaagtggt caacatcatc aatgccatgg
    24061 aaccggagat tgaaaaactc tccgacgaag aactgaaagg aaaaacggca gagttccgcg
    24121 cgcgtctgga aaaaggcgaa gtgctggaaa acttgatccc ggaagccttc gccgtggtgc
    24181 gtgaagccag taagcgtgtt tttggcatgc gccacttcga cgtacagctg ctcggcggta
    24241 tggttcttaa tgaacgctgc atcgcggaaa tgcgtaccgg tgaaggtaaa accctgaccg
    24301 caacgctgcc tgcttacctg aacgcactaa ccggtaaagg cgtgcacgtc gttaccgtca
    24361 acgactacct ggcgcaacgt gacgccgaaa acaaccgtcc gctgtttgaa ttccttggcc
    24421 tgactgtcgg tatcaacctg ccgggcatgc cagcaccggc aaagcgtgaa gcctacgctg
    24481 ctgacatcac ttacggtacg aacaacgaat acggctttga ctacctgcgt gacaacatgg
    24541 cgttcagtcc tgaagaacgt gtacagcgta aactgcacta tgcgctggtg gacgaagtgg
    24601 actccatcct gatcgatgaa gcgcgtacac cgctgatcat ttctggcccg gcagaagaca
    24661 gctcggaaat gtataaacgc gtgaataaaa ttattccgca cctgatccgt caggaaaaag
    24721 aagactccga aaccttccag ggcgaaggcc acttctcggt ggacgaaaaa tctcgccagg
    24781 tgaacctgac cgaacgtggt ctggtgctga ttgaagaact gctggtgaaa gaaggcatca
    24841 tggatgaagg ggagtctctg tactctccgg ccaacatcat gctgatgcac cacgtaacgg
    24901 cggcgctgcg cgctcatgcg ctgtttaccc gtgacgtcga ctacatcgtt aaagatggtg
    24961 aagttatcat cgttgacgaa cacactggtc gtaccatgca ggggcgtcgc tggtctgatg
    25021 gtctgcacca ggctgtggaa gcgaaagaag gtgtgcagat ccagaacgaa aaccaaacgc
    25081 tggcttcgat caccttccag aactacttcc gtctgtatga aaaactggcg gggatgactg
    25141 gtactgctga taccgaagct ttcgaattca gctccatcta taagctggat actgtcgttg
    25201 ttccgaccaa ccgtccaatg attcgtaaag atctgccgga cctggtctac atgactgaag
    25261 cggaaaaaat tcaggcgatc attgaagata tcaaagaacg tactgcgaaa ggccagccgg
    25321 tgctggtggg tacaatctcc atcgaaaaat cggagctggt gtcaaatgaa ctgaccaaag
    25381 ccggtattaa gcacaacgtc ctgaacgcca aattccatgc caacgaagcg gcgattgttg
    25441 ctcaggcagg ttatccggct gcggtgacta tcgcgaccaa catggcgggt cgtggtaccg
    25501 atattgtgct cggtggtagc tggcaggcag aagttgccgc gctggaaaat ccgactgcag
    25561 agcaaattga aaaaattaaa gccgactggc aggtacgtca cgatgcggta ctggcagcag
    25621 gtggcctgca tatcatcggt actgaacgtc acgaatcccg tcgtatcgat aaccagctgc
    25681 gcggtcgttc tggtcgtcag ggggatgctg gttcttcccg tttctatctg tcgatggaag
    25741 atgcgctgat gcgtattttt gcctccgacc gagtatccgg catgatgcgt aaactaggta
    25801 tgaagccagg cgaagccatt gagcacccgt gggtgaccaa agcgattgcc aacgcccagc
    25861 gtaaagttga aagccgtaac ttcgacattc gtaagcaact gctggaatat gatgacgtgg
    25921 ctaacgatca gcgtcgcgcc atttactccc agcgtaacga actgctggat gtcagcgatg
    25981 tgagcgaaac catcaacagc attcgtgaag atgtgttcaa agcgaccatt gatgcctaca
    26041 ttccgccaca gtcgctggaa gaaatgtggg atattccggg gctgcaggaa cgtctgaaga
    26101 acgatttcga cctcgatttg ccaattgccg agtggctgga taaagaacca gaactgcatg
    26161 aagagacgct gcgtgagcgc attctggcgc agtccatcga agtgtatcag cgtaaagaag
    26221 aagtggttgg tgctgagatg atgcgtcact tcgagaaagg cgtcatgctg caaacgcttg
    26281 actctctgtg gaaagagcac ctggctgcga tggactatct gcgtcagggt atccacctgc
    26341 gtggctatgc acagaaagat ccgaagcagg aatacaaacg tgaatcgttc tccatgtttg
    26401 cagcgatgct ggagtcgtta aaatatgaag ttatcagcac gctgagcaaa gttcaggtgc
    26461 gtatgcctga agaggttgag gagctggaac aacagcgtcg tatggaagcc gagcgtttag
    26521 cgcaaatgca gcagcttagc catcaggatg acgactctgc agccgcagct gcactggcgg
    26581 cgcaaaccgg tgaacgaaaa gtaggacgta acgatccttg cccgtgtggt tctggtaaaa
    26641 aatacaagca gtgccatggc cgcctgcaat aaaagctaac tgttgaagta aaaggcgcag
    26701 gattctgcgc cttttttata ggtttaagac aatgaaaaag ctgcaaattg cggtaggtat
    26761 tattcgcaac gagaacaatg aaatctttat aacgcgtcgc gcagcagatg cgcacatggc
    26821 gaataaactg gagttttccg gcggtaaaat tgaaatgggc gaaacgccgg aacaggcagt
    26881 ggtacgtgaa cttcaggaag aagtcgggat taccccacaa catttttcgc tatttgaaaa
    26941 actggaatat gaattcccgg acaggcatat aactctgtgg ttctggctgg tcgaaagctg
    27001 ggaaggggag ccgtggggta aagaagggca acccggtgag tggatgtcgc tggtcggtct
    27061 taatgccgat gattttccgc cagccaatga accggtaatt gcgaaactta agcgtgtgta
    27121 tgtcgaataa ggcgtcacgc tgcatccgac atttgcatat gatgcctgat gcgacgctgg
    27181 cgcgttttat caggccagca tcgcatcaga tagaatgtca ctgctttggc tcttcgctcc
    27241 agtcatcgct ttcggaaaga tcgccactgc tggggattcg tttttcttca gcagcccatt
    27301 ctccgaggtc gatcagctga caacgtttgg agcaaaatgg ccgaaacggg ctgatttcac
    27361 cccacaccac cgttttcccg caggttgggc aattcaccgt aatagtttct gacattttta
    27421 ctccttagca acaggccagt tcgaaatcca gacgttctgg tacttgtccg ttttcactgt
    27481 ccagcggcat aaaacgaatg gcaaaacggc tcttatgacc ggaaatttgc ggataaagct
    27541 gtgaatcgag cgacagattc aggcgcagca agtcggcatc gccaccgtta tcctgataaa
    27601 aaccattcag gctggtttgt ttacggaagg gggccgactg gcgaattaaa tccagcacca
    27661 tggtaagtgc ctgggtaagt gggttcaggc tggcaatcca ggtttctacc tggctgtcgc
    27721 gctgcgcctg gggtagatgc agccaaatgt gcaatgtagg taaatcaaag ctgcaacagc
    27781 cgcccgggat gctcagtcgc tgacgcacca gagcaatcaa acgatcttca cgtagaaatt
    27841 gcccgatacg cggcgcggaa attaatacgc tccccgccgc ttttaactgc tgaattaatg
    27901 cttcaatacg gctctggtcc acgccgggca cgccaatcca ggtctggagt ttacgttgct
    27961 gccggtcaag ttctttcaac agctcagtgc ggacttcgcc gcgctcgaaa acatccagta
    28021 attcgctgac attacggaag aaatgcagcg cgccagcgtg gtcaacgatg ggtaaattaa
    28081 cggtgagttg ctgaatcaaa aactcaatgc gcagccatgt acgcattttt tcatttagtg
    28141 gatgttcaaa aaggacctgg gtctgcatta cggtttttcc tgtgagacaa actgcgacgc
    28201 aagctgcaaa tagtgtgcgt gcaggcgggc aacatccgat gcgatagcat ccggtgcgcc
    28261 gttattatca atgacgtcat ctgccacggc aaggcgagct tcgcgcgttg cctgagcagc
    28321 aaggatttgt tcgacatgct cgcgagttac atcgtcgcgc tgcatggtgc gcttaagttg
    28381 cgtttctggg ctgacatcca ccaccagcac tcgattcgct tttttataca gtgagttttc
    28441 taccagcaat ggcacaaccc atagtacata gggggaagta gcttgctgga tctggtgttg
    28501 cgtctcttgc tgaatcagcg gatgcagcag ggcgttaagc cagtttttct cttccgggtt
    28561 ggcgaagatc cgctcgcgca aggcccggcg ctgcaatgtt ccatcagcag caatcatgtt
    28621 agcgccaaag tgatcagcaa tggcatgtag cgcaggtgca cctggttcaa ccacctgacg
    28681 cgcaataata tcggcatcaa tgacgttaat tccgagatca gcaaacgcat tggcaacggt
    28741 actcttgcca ctgccaatgc ctcccgttaa ggcaactata tacctcataa tttatattcc
    28801 cgggaattca tcatgattat caaaacgtta aaaatgagcg cacgaaagcg aaattgatga
    28861 aacgttcgct cactatttac caggtaaatt tatgggattg tagcgtaaaa aaagacaatt
    28921 tcgcagtctt gcgccgcatt gattagtgcg tatgataacg tcactggagt tgcgctctta
    28981 cccttatagc cattaacccc aggaatccgc acatgcgtat tgaagaagat ctgaagttag
    29041 gttttaaaga cgttctcatc cgccctaaac gctccactct taaaagccgt tccgatgttg
    29101 aactggaacg tcaattcacc ttcaaacatt caggtcagcg ctggtccggc gtgccaatta
    29161 tcgccgccaa tatggacacc gtaggcacat tttctatggc ctctgcgctg gcttcttttg
    29221 atattttgac tgctgtgcat aaacactatt ctgtcgaaga gtggcaagcg tttatcaaca
    29281 attcttccgc tgatgtgctg aaacatgtga tggtttctac cggcacgtct gatgcggatt
    29341 tcgaaaaaac taaacagatt ctcgacctga acccggcatt aaacttcgtt tgtattgacg
    29401 tggcgaatgg ttattccgaa cacttcgtgc agttcgttgc gaaagcgcgt gaagcgtggc
    29461 cgaccaaaac catttgtgct ggcaacgtag tgactggtga aatgtgtgag gagcttatcc
    29521 tctccggtgc tgatatcgtt aaagttggca ttggcccagg ttctgtttgt acaactcgcg
    29581 tcaaaacagg cgtcggttat ccgcaacttt ctgcggtgat cgaatgtgcc gatgctgcgc
    29641 atggtctggg cggaatgatc gtcagcgacg gtggctgcac cacgccgggc gatgtcgcga
    29701 aagcctttgg cggcggtgcc gatttcgtca tgcttggcgg catgctggcg ggccacgaag
    29761 agagcggcgg tcgcatcgtt gaggagaacg gcgagaaatt tatgctgttc tacggcatga
    29821 gctcagagtc tgcgatgaaa cgtcacgttg gcggtgttgc ggaatatcgc gcagcagaag
    29881 gtaaaaccgt taagctgccg ctgcgaggcc cggttgaaaa taccgcgcgt gatattttgg
    29941 gcggcctgcg ttcagcttgt acatacgttg gggcttcacg cctgaaagaa ctgaccaagc
    30001 gcaccacatt tatccgtgtg caggaacaag aaaaccgcat cttcaacaac ctgtaatata
    30061 ccaacgctgg cgtggagcaa cacgccagcg ttatcccatt ccactcatcg catcgcctaa
    30121 atggaaaatt ggcagataca ttgccaccac cagcgtaccg ataattcctc ccgttatgat
    30181 cagcaacgcc ggttccagta aggctgcgag gttatccgcc agcgccattg tgttatcccg
    30241 atgatgatgg gcgaggttgt ctaacatgag atccagcgag ccggatgcct ctcctgttct
    30301 cactaattgc aaacagagcg ggctaaactc accggcattt tttagcgcca gccagatggg
    30361 gtgaccgtta ctgatatcgt gctggatttg tgtcagaagt tgcacccagt acggacagcg
    30421 cattgtttct ctgacgctct caacgccctg taaaaaagaa atgcctgcac tttgtgtcag
    30481 cgtcagaatc gtaaagatct gcgtgagttt ttgtccccgc atcagtgaac ccataatcgg
    30541 gatgcgtaac agtaatttct gccgtgcgat aagcctggtt gggtggcgca tcagcagctt
    30601 attggctatc gccagcagaa agccgaatag caccagcaac caaccccatt cgccactaaa
    30661 acttgccagc gtcatgatcc cctgcgttag cgccggtagc ggagtgttaa aggtcttata
    30721 gatagcggca aactccggca gaacaaaatg cagcattgcc acaaccacca tgattgccat
    30781 cgctaaaatg ataatgggat aacgtaacgc tgatttcact ttgtcggtca actgacgctg
    30841 ggctttttgc tgacgcgcca gttcaaagca gcattcatcc agcttaccgg tcagttcacc
    30901 tgtgcggatc atcgcctgat agagcggcgg aaatacctct gaccagggta ataaggcatt
    30961 ggaaaaggca acgccctgtt caagatcgtg cgccagtgat tgcagcaacg cttgccactg
    31021 cttactggga tgctgttccg ccagcagagc cagcccttca gaaagcgtta acccggcttt
    31081 gagtagcgtc gccagttgat gaatgacttc cgcgctttta tctcctcgcc actgcgcaga
    31141 attgatggcg attcgcttca ggcttagtgg ggtaaccatc tgttgctgta gtgccatgag
    31201 cagcaaagca cggctctctg cccatagcat cccatcttgc gcattgccgt cgccggttat
    31261 gccacgccag cgccagagtt gcttactcgc catgcggcat ccccaatacg cggattaact
    31321 cttcaaaggt ggttaagcct tgctccacag ccaggcagcc gttttcaaaa agcgttcgca
    31381 tacccgcctg tcgggcgtgc gtttccagcg attcaacgtc ggtattagcg gaaataagct
    31441 gacgtatgac cggtgttatg ggcagaactt caaataaggc cgtgcgacca taaaaaccgt
    31501 ggtagcaatg tacacaaccg ggtgcctgtc agtggggcag cggcgacggc catacattgt
    31561 ctggaatatg gatgggctcc ccttgctgcc ggcgacaatg cgggcaaagt ttgcgtacca
    31621 gacgctgggc tattaccagc gtaagcgccg atgatagcat ccagcgggcg actcccattt
    31681 gctgtaaacg taccagcgtt tcgcaggtgg aattagtgtg tagggtagac aacaccaggt
    31741 gaccggtttg cgcggctttt atggcaattt ctgccgtttc gccatcgcgg atctctccga
    31801 tcatgatgac gtcaggatcc tggcgcaata acgcacgcaa aacgccctga aaggtgagtc
    31861 cggcacgcga atggatttgc gtctggttta atccggctat ggggatctca accggatctt
    31921 cgacgctaca aatattaatg tcagcggtat tcagcgtttg cagggcacta taaagcgtga
    31981 ccgttttgcc gctgcctgta gggccagtta ccagcaccag tccctgtggt tgttgcaagg
    32041 catgagcaaa gtccaccagt tgtgacggct gcattccaag cgtgttaaca tccagtgcct
    32101 ggttcacctg ctgtaacaac cttaatacca ccttttcacc accccgacat gctaaggtcg
    32161 caatacgaaa tgagacggcg tttcctgcca gttcgacagt gaattgcccg tcctgcggca
    32221 ggcgatgttc cgcaatatcc aggtttccca gcacttttaa tctggcggtt aatgcgactc
    32281 cggcatccgg tgaaacgtcc ggtaaaggat gcaatacgcc gtcgatacgc aagcggatgc
    32341 ggtaggcatt gtccgctggt tcgatatgaa tatcagacgc gcgttgttct agcgcagatt
    32401 gtaacgttcg agtcagcaac tctgctttgg gctgatgctt ctcctgaaca gctacgggca
    32461 atgtctgttg tgtgcgactg gcgtgacctt ccatttgttg gcgcgtccag caggtgatct
    32521 caatacgttt ggtggtagcg aaatgcaatg cgtccagtag ctcatgcgaa ggtgcatcga
    32581 ctaccgcaac atgaaccacc tcttcgctgg catccagcaa gactccctga taacgcagac
    32641 acagggcagt gagctgtgga atattcattg ccgctcctta gttggcgtca tcaaagcgga
    32701 agacatcttc gcaggcttgc tgcaatgcgc tgtcactttg aatattgcag ttacgcgtcc
    32761 agccggtgac gccgtttgcg ttatcccaac ccggtgtcat gacgacgctt agcccattga
    32821 gactttcttg cccggtcagc gacaccacgc cctttgccac actcatggct gaaacatagc
    32881 gggtggtggt aggcgaagga atgccattgc tgccaccgtc gcaggtattt aatccaccat
    32941 gttccagcgc gcacaactct acggcggtac ggtaaggcac aaaggtttgt agcatgtcgg
    33001 tgagtgcggc tttgcgcagg tagttttgat aagcgggaat accaatggcg cttaaaatgg
    33061 caatgatgcc aataaccacc atcagttcaa taagtgtaaa accgcgttgc ttgtccatct
    33121 gtttcgctcc ttgatttggt tggcgctact ttggcaaacg ccatcagcac aagcgagggg
    33181 caaaaaacga aacgggaaag cagcttccga ggttttttac ttcgttgcag cgaaagacaa
    33241 gaaatttgcg aggcgttacg aagaaagttg gggaagggga gatttatccg cccgcgatgg
    33301 agcggataaa tctgttagca attagcgaaa acgcattgaa aggtcgagtg cctgtacgtg
    33361 tttagttagt gcaccaacgg agataaagtc cacgcccgtt tcggcaaatt cgcgcagtgt
    33421 tttgtcagtg acgttgccag acacttccag tagcgccttg ccgttggtgc gtttgacggc
    33481 ttcgcgcatc tgttctgttt cgaagttatc cagcatgatg atatcggcac ctgctttcag
    33541 ggcttcatca agttcttcca gattctctac ttcgacttct actggcgcat ccgggtgcaa
    33601 ccaggacgct ttttcgaccg cctgccgcac tgagccggag gcaataatat ggttttcttt
    33661 gatcaggaag gcatcagaaa gccccagacg gtgattcgct ccgccgccgc aaagtaccgc
    33721 gtatttcaga gctgaacgca ggccgggtaa ggttttgcgc gtatccaaca gctgcgtgtt
    33781 ggtgccttcc agtaattcga catagtggcg taccttactg gcaactcctg aaagggtttg
    33841 cacaaaatta agcgcagtgc gttcgcccgt taacagcacg cgagatgggc cttcaagttc
    33901 gaacaagggt tgattggcgt tgatgacatc gccgtcatcc acatgccaga ttatggtgac
    33961 atcgtcgcct gccagttgaa taaacacctc ttcaacccag cgtttgccgc aaaatacgcc
    34021 attctcgcgg gtgatcactg tggcatgaga gcgagagttt tccggtaaaa gttttgccgt
    34081 aatgtcattg ttggcatcga ctgttccgcc taaatcttcc cgcagcgcct gggccaccgc
    34141 gccggggata tcgagattaa tgcgttccag cagctcgtca cgtcgggtgt cagggttata
    34201 gcggcgaggc ggcatgttaa aactccagat agctaacgaa tcataaggta gaaacatgct
    34261 actctgaacc gggtattagc accacatata aggagatcct gcatgttgtt agaacagggg
    34321 tggctggctg gcgcgcgccg cgttccctca ccacattacg attgccgccc ggatgacgaa
    34381 acacccaccc tgctggtggt gcacaatatt agcctgccgc caggcgagtt tggcggtccg
    34441 tggatcgacg cattattcac tggaactatt gatccgcagg cgcatccttt ctttgcggag
    34501 atcgcccatt tgcgcgtctc cgctcactgt ttgattcgcc gtgatggtga aatagtccag
    34561 tatgttcctt tcgataaacg agcctggcat gcgggagtct ctcagtatca ggggcgcgaa
    34621 cgctgcaatg atttttctat tgggattgag ctggaaggca ccgatacgct ggcgtatacc
    34681 gatgcgcagt atcaacagct tgcggcggtt acacgggcac tgattgatcg ctatccggat
    34741 atcgctaata acatgacggg ccattgtgat attgcgccgg atcggaaaac cgatcccggt
    34801 cctgcatttg attgggcacg gtttcgtgcg ctggtcagca aggagacaac atgacgctat
    34861 ttacaacctt actggtgtta attttcgagc gcctgtttaa gttgggcgag cactggcagc
    34921 ttgatcatcg tcttgaagcg ttctttcggc gggtgaaaca tttttctctc gggcgcacgt
    34981 tatgcatgac cattattgcg atgggcgtga cttttttact gttacgcgca ttgcagggtg
    35041 tattgttcaa cgttcccacg ttgctggtgt ggctgctgat tggtttgctg tgtattggcg
    35101 caggtaaagt tcgtcttcat tatcatgctt atctgacagc tgcttcacgt aatgatagcc
    35161 atgcccatgc cacgatggct ggcgaactca ccatgattca cggcgtcccg gcaggctgcg
    35221 atgaacgtga gtatttgcgt gagctgcaaa atgcattgct gtggattaac tttcgttttt
    35281 atcttgctcc gctgttctgg ctgattgtgg ggggaacctg gggacccgtt acgctgatgg
    35341 ggtatgcgtt tttgcgtgca tggcaatact ggctggcgcg ataccagacg ccgcatcatc
    35401 gtttacagtc cggcattgat gccgtgcttc atgcactgga ttgggtgccg gttcgtcttg
    35461 cgggtgtggt atatgccttg atcggtcatg gtgagaaagc gttaccggcc tggtttgctt
    35521 cgctgggtga tttccatact tcgcaatatc aggtattaac gcgtctggca cagttctctc
    35581 tggcgcgtga accgcatgtc gataaggtgg agacgccaaa ggccgcggtt tcaatggcga
    35641 agaaaacctc gttcgtggtc gtggtggtaa ttgcgctact gacgatttac ggggcgttgg
    35701 tgtaaagatt attgccctca ccctgcacgg gtgagggcgt agagagatta atgcgctttt
    35761 acggctttgg cggttttctc tttaaacaga tagccgatac ctaacacgat cagccatacc
    35821 gggatcaggt ataccgaaat cgccattcct ggggtcatca gcataatcac cagtaccgcc
    35881 gccataaaca gcaggcagat ccagttaccc agcggataaa gcagggcagg gaagcgagtt
    35941 accacgcctt gttcttgttt ggcacgacgg aatttcatat gcgccagact gatcatcgcc
    36001 cagttgatga ccagtgcaga taccaccagc gccattaaca gtccgaaagc ggactctggg
    36061 gcaaggtagt taatcagtac gcacaatgcc gtaaccagcg cagacaccag aatagtgtta
    36121 accggtacgc cgcgtttatc gacagacgcc agcgcttttg gcgcgttccc ttgttgtgcc
    36181 agaccgaaca gcatacggct gttgcaatat acgcagctgt tgtacacgga gagcgcagca
    36241 gtcagtacca cgatattcag cgcattcgct acaaaggtat cgcctaactc gtggaagatc
    36301 agcacaaacg gactggtatc ggcggtaacg cgggtccacg gcattagtga gagcagaacg
    36361 gctaacgagc caatatagaa aatcaggatg cggtagataa cctggttagt tgctttcggg
    36421 atactttgct ccgggttatc agcttctgct gcggtgatcc ccaccagttc cagaccaccg
    36481 aaagagaaca tgataatcgc catcatcatc accagaccgg tgaagccgtg tggcaggaaa
    36541 ccaccctgat cccacaggtt gctaacgctc gcctgcgggc cgccgttgcc gctgaatagc
    36601 agccaggcgc cgaagatgat catcgctacc accgcgataa ctttgataat ggcaaaccag
    36661 aactccatct cgccaaacac ttttacgttt gtcaggttga tggcgttgat caccacaaag
    36721 aacacggcgg cagaaaccca ggttgggatt tccggatacc agaactgaat gtatttaccc
    36781 acggcagtca gctctgccat ggcaactaaa acgtacagta cccagtagtt ccagccagag
    36841 gcgaaaccgg caaaactgcc ccagtattta taagcaaagt ggctaaagga gcctgcgaca
    36901 ggttcttcga ccaccatttc acctagctga cgcatgatca gaaaggcgat aaaaccagca
    36961 atggcgtaac ccaggataat ccctggccct gcggactgta ttacggaggc gctacccagg
    37021 aatagccctg tccctatcgc gccacccagc gcgataagct gaatatggcg gtttttaagg
    37081 ccgcgcttta gctgctcgcc gtgctgttga ccttccatta tgaaacctcg tgttgtgttg
    37141 tttttttgaa ctacgcagca atgcgtgtgt aattttgtaa ttccgtttgt tgtattggtt
    37201 tctttacatt ttatgtaact gaattatgat gatagtactt tcaactatca caagataatg
    37261 gtatacgcgc gagaatgcac ctgctttatg tagatgtgtt gattttctgg ataaaaaaaa
    37321 agcaattgta agtttttcat cacatttaat agatacttta agtctgataa catactgttt
    37381 taactagcta tataccggat ttgtctgcct gctgaacacc atgccttagc caccagcaca
    37441 ataatcttac aaaaggtcat ataagaataa atagatgt
//
LOCUS       DAPQZD010000032        36224 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPQZD010000032 DAPQZD010000000
VERSION     DAPQZD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.032"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(128..415)
                     /locus_tag="RO454_001543"
     CDS             complement(128..415)
                     /locus_tag="RO454_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDY0933390.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(490..633)
                     /gene="mgrB"
                     /locus_tag="RO454_001544"
     CDS             complement(490..633)
                     /gene="mgrB"
                     /locus_tag="RO454_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDY0933391.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            792..1031
                     /locus_tag="RO454_001545"
     CDS             792..1031
                     /locus_tag="RO454_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933392.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1175..1966)
                     /gene="kdgR"
                     /locus_tag="RO454_001546"
     CDS             complement(1175..1966)
                     /gene="kdgR"
                     /locus_tag="RO454_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDY0933393.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2143..3516
                     /locus_tag="RO454_001547"
     CDS             2143..3516
                     /locus_tag="RO454_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933394.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3562..4443)
                     /gene="htpX"
                     /locus_tag="RO454_001548"
     CDS             complement(3562..4443)
                     /gene="htpX"
                     /locus_tag="RO454_001548"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDY0933395.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4635..6683)
                     /gene="prc"
                     /locus_tag="RO454_001549"
     CDS             complement(4635..6683)
                     /gene="prc"
                     /locus_tag="RO454_001549"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDY0933396.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6703..7401)
                     /gene="proQ"
                     /locus_tag="RO454_001550"
     CDS             complement(6703..7401)
                     /gene="proQ"
                     /locus_tag="RO454_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDY0933397.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7498..7995)
                     /gene="msrC"
                     /locus_tag="RO454_001551"
     CDS             complement(7498..7995)
                     /gene="msrC"
                     /locus_tag="RO454_001551"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDY0933398.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8125..9408
                     /gene="yebS"
                     /locus_tag="RO454_001552"
     CDS             8125..9408
                     /gene="yebS"
                     /locus_tag="RO454_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDY0933399.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9377..12010
                     /gene="yebT"
                     /locus_tag="RO454_001553"
     CDS             9377..12010
                     /gene="yebT"
                     /locus_tag="RO454_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDY0933400.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12090..13529
                     /gene="rsmF"
                     /locus_tag="RO454_001554"
     CDS             12090..13529
                     /gene="rsmF"
                     /locus_tag="RO454_001554"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDY0933401.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13646..13882
                     /locus_tag="RO454_001555"
     CDS             13646..13882
                     /locus_tag="RO454_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDY0933402.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13987..14178
                     /locus_tag="RO454_001556"
     CDS             13987..14178
                     /locus_tag="RO454_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDY0933403.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14179..14835)
                     /gene="pphA"
                     /locus_tag="RO454_001557"
     CDS             complement(14179..14835)
                     /gene="pphA"
                     /locus_tag="RO454_001557"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDY0933404.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15230..15571)
                     /locus_tag="RO454_001558"
     CDS             complement(15230..15571)
                     /locus_tag="RO454_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDY0933405.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15584..16456)
                     /gene="copD"
                     /locus_tag="RO454_001559"
     CDS             complement(15584..16456)
                     /gene="copD"
                     /locus_tag="RO454_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDY0933406.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16460..16834)
                     /gene="yobA"
                     /locus_tag="RO454_001560"
     CDS             complement(16460..16834)
                     /gene="yobA"
                     /locus_tag="RO454_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDY0933407.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16973..17203
                     /gene="holE"
                     /locus_tag="RO454_001561"
     CDS             16973..17203
                     /gene="holE"
                     /locus_tag="RO454_001561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDY0933408.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17305..17961
                     /locus_tag="RO454_001562"
     CDS             17305..17961
                     /locus_tag="RO454_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDY0933409.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17985..18647
                     /gene="exoX"
                     /locus_tag="RO454_001563"
     CDS             17985..18647
                     /gene="exoX"
                     /locus_tag="RO454_001563"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDY0933410.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18644..20704)
                     /gene="ptrB"
                     /locus_tag="RO454_001564"
     CDS             complement(18644..20704)
                     /gene="ptrB"
                     /locus_tag="RO454_001564"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDY0933411.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20913..21572)
                     /locus_tag="RO454_001565"
     CDS             complement(20913..21572)
                     /locus_tag="RO454_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDY0933412.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21899..22255)
                     /locus_tag="RO454_001566"
     CDS             complement(21899..22255)
                     /locus_tag="RO454_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDY0933413.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22322..22612)
                     /gene="yebG"
                     /locus_tag="RO454_001567"
     CDS             complement(22322..22612)
                     /gene="yebG"
                     /locus_tag="RO454_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDY0933414.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22746..23924
                     /gene="purT"
                     /locus_tag="RO454_001568"
     CDS             22746..23924
                     /gene="purT"
                     /locus_tag="RO454_001568"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDY0933415.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23980..24621)
                     /gene="kdgA"
                     /locus_tag="RO454_001569"
                     /gene_synonym="eda"
     CDS             complement(23980..24621)
                     /gene="kdgA"
                     /locus_tag="RO454_001569"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDY0933416.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24658..26469)
                     /gene="edd"
                     /locus_tag="RO454_001570"
     CDS             complement(24658..26469)
                     /gene="edd"
                     /locus_tag="RO454_001570"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDY0933417.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26704..28179)
                     /gene="zwf"
                     /locus_tag="RO454_001571"
     CDS             complement(26704..28179)
                     /gene="zwf"
                     /locus_tag="RO454_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDY0933418.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28517..29386
                     /gene="hexR"
                     /locus_tag="RO454_001572"
                     /gene_synonym="yebK"
     CDS             28517..29386
                     /gene="hexR"
                     /locus_tag="RO454_001572"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDY0933419.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29514..30956
                     /gene="pyk"
                     /locus_tag="RO454_001573"
     CDS             29514..30956
                     /gene="pyk"
                     /locus_tag="RO454_001573"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDY0933420.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31088..32059)
                     /gene="lpxM"
                     /locus_tag="RO454_001574"
                     /gene_synonym="msbB"
     CDS             complement(31088..32059)
                     /gene="lpxM"
                     /locus_tag="RO454_001574"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDY0933421.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32178..33500)
                     /gene="mepM"
                     /locus_tag="RO454_001575"
     CDS             complement(32178..33500)
                     /gene="mepM"
                     /locus_tag="RO454_001575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDY0933422.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33516..34448)
                     /gene="znuA"
                     /locus_tag="RO454_001576"
     CDS             complement(33516..34448)
                     /gene="znuA"
                     /locus_tag="RO454_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDY0933423.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34527..35282
                     /gene="znuC"
                     /locus_tag="RO454_001577"
     CDS             34527..35282
                     /gene="znuC"
                     /locus_tag="RO454_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDY0933424.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35279..36064
                     /gene="znuB"
                     /locus_tag="RO454_001578"
     CDS             35279..36064
                     /gene="znuB"
                     /locus_tag="RO454_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDY0933425.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
BASE COUNT         8668 a         9292 c         8923 g         9341 t
ORIGIN      
        1 ataatatgat aattatttga agtcagacca gagttttaat ttgaagcagt tgcaccatga
       61 cagtgcgtat aatcagcatg aattgagtaa cccttcaacg acggggttac tcaagattga
      121 ggaaggatta tcgttcggcg accagacgaa taatatcttc gtcttcaacc gatttttgac
      181 tctcaacggt ctttttatct gctttctcag gttcgtttgc ttcgcacaaa cgacgtaaca
      241 gtgcattttg acgtttttgc tgatccagca acgcctcaag cagttcaatc tgttcgttag
      301 tccgtgaact ggcacgattg ataaaaaacc acaagatgag cccgataaga agaaccacca
      361 ccgatacaac caaagacgca atgttcatca cgcctgaatt tacaacttcg ttcatttcac
      421 cacctctgag tagaggcgct attctaccac tgctggagag gaagaaaatc cagtgctgaa
      481 aaaatgatat caccacggga taaactggtt aatggcacaa attccgctga aaaattgtac
      541 atcctgatcg cacatcatgt tgaatacctg cgcccaaagc agcaagcaag ccaacaccac
      601 gacaaccaga gcgacccatc gaaacttttt cactccaatc tccgtttcac ctaccttatg
      661 tcattaaact agcatgcgtt agttaaacag cgcctaacta tgtcgatatt cgtgcttttt
      721 cagaatgagt cgcttgcttc agcgcatatt gccgctacga ttaagcgaac ataaaaagag
      781 aagaggttgt aatgcgattc atcattcgca cagttatgct gatagcactg gtatggatag
      841 gattattact cagcggatat ggcgttttga ttggaagcaa agagaatgcc gcgggattag
      901 gtttgcaatg tacctatctg accgccagag gcaccagcac ggtacaatac ctgcacacta
      961 aaagtggttt tctggggata acagattgcc cgttgttacg caaaagcaat attgtggtcg
     1021 ataatggtta agggtaataa ataaccagat tctcactcat gagtaaaata ttcttatcgc
     1081 aagtgcgttt aatataacgc caccttcaac tttatttttc tcatgctaaa taaagaacct
     1141 gtctgtttga tattcagaca ggttcttact ctcatcagaa cggatagtcg tgataaccca
     1201 tttgggcaga aattttgcgc gccgcggtat gcaacattgc gacatattcc tgtaaacgct
     1261 cttcagagaa acgcaacgtc gggaaggaga tgctcagacc ggcaatgacc acgccaaagc
     1321 gatcaaatac cggaaccgca atgcaacgca gtccttcttc ctgctcttcg ttatcttcgc
     1381 catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta ctgatgatgg
     1441 tccgttcggt actgcgttta tactccacgc cctcaagaat ttgcatcact tcatcgcgat
     1501 cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta cgacgcccaa
     1561 tccgtgaata catgcgcaaa ttgtacattg agtcaatttt gtggatgtaa acaatactgt
     1621 cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc tcacgcatct
     1681 ggatatctgc gctacgaatt aaatcgacgt tttgcaacgc gcgggcgccc agttcaaaca
     1741 atttcagggt cagcgaatat ttctccgact ccccttcctg cgccacataa cctaaggttt
     1801 tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc tgcgacaact
     1861 cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa acttttagca
     1921 cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat cacctcattg
     1981 cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcacctg ggtggtcgca
     2041 atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat cagtgctaga
     2101 atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa agttcaggcc
     2161 gacggcctgc cattgcccct gcgatacggt gcgatattaa ccattgtgat tggtatttcg
     2221 atggctgtcc ttgacggcgc aatcgccaac gtcgccttgc caacaatcgc tacggacctt
     2281 catgccacgc cggccagttc catctgggta gtgaacgcct atcaaatcgc cattgtcatc
     2341 tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat ttataaatgc
     2401 ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc gctgcaaatg
     2461 ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag cgttaatacc
     2521 gcacttatcc gcctgatcta tccacaacgt tttctgggta gagggatggg cataaactcg
     2581 tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat cctctccatc
     2641 gcatcctgga aatggttatt tttaatcaac gtaccgttgg gtattatcgc cctgcttctg
     2701 gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt cgacctgccc
     2761 agcgccgtga tgaacgcgtt aaccttcggc ctgcttatta ctgcattgag tggtttcgct
     2821 caggggcaat cgctgacatt gattggtgcg gaactggtgg taatggttgt cgttggtatt
     2881 ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga tttactgcgt
     2941 atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc acaaatgctg
     3001 gcaatggttt cccttccctt ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca
     3061 ggtttacttc tgacaccgtg gccgttagca acgatggtta tggctccact ggcaggctat
     3121 ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttattcat catggctgcg
     3181 gggctttttt ccctggttct gctgccagcg tcacctgcgg atatcaatat tatctggccg
     3241 atgatcttat gtggtgctgg atttggcttg ttccagtcac ccaataacca caccattatt
     3301 acctccgctc ctcgcgaacg tagcggtgga gccagtggca tgttagggac ggctcgtctt
     3361 ctgggtcaga gtagcggcgc ggctctggta gcgctgatgc taaatcagtt tggtgataat
     3421 ggtacgcacg tctcgctgat agctgcggct attctggcag tgattgctgc ctgtgtcagt
     3481 ggtttacgta tcactcagcc acgatccagg gcataaaaaa agcgcgtcga tcaggacgcg
     3541 ctttttagta tttacgtcat attacttcag gtattcaccc gtacgcagag cttcaatacg
     3601 tttatccagc ggcgggtggg tcatgaacaa ctcactgagc gatttcgact taccgttaat
     3661 gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca ggcgttgcag
     3721 cgcggcaatc attttctcgc gaccaaccag ttttgctgaa cccgcatcag catggaattc
     3781 acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca ccagttccag
     3841 aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac cttcatcacg
     3901 gtttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa agataacgaa
     3961 ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg cgatgtggct
     4021 gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca gcaaaccagt
     4081 gctgacagca accagagagg catcacggcg cgcaccagtt gcaaaagcat tgatgtccgg
     4141 cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct gggttgctac
     4201 agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt ccccgccaac
     4261 agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac caaaaccgaa
     4321 cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg tcaggctcag
     4381 taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg cgattcgcat
     4441 cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca catcgtatgg
     4501 gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac acaactttac
     4561 attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc acaatttctt
     4621 gtgcctgata gatattactt gacgggagcg ggttgttccg cgggtctggc tttttcaagc
     4681 ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatctgg ctcctggtaa
     4741 tctttcggta gatcatccag tttcttcaac tccggtttac cttcgcgttt aaagcgttcg
     4801 ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg cacagcgtaa
     4861 ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat atccttcatg
     4921 atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag cagctccggt
     4981 tcaaaggccg ttaaatctcc tgatttcaca taagtcgcgg catcaatgct atcccacggc
     5041 agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt cggcatgatg
     5101 atgtctggcg ttacgccttt acgttgcgta ctgccgccgt taacgcgata gaacttctgg
     5161 atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg atcgtaaata
     5221 cggttcaatg aacggtactg ctgaacggtg cctttaccaa acgtcggttc acccacaacc
     5281 agggcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga agcactgaag
     5341 cggtcaacca gcaccaccag cgggccttta tagaagacct gtccgtcggt gtcgctgtct
     5401 tcacgaacct tgccgttgtt atcgcggacc tgaacaatcg ggccggatgg aatgaacaga
     5461 ccggagagcg atacggcttc agtcaacgcc ccaccaccat tgctacgcag gtcgataatc
     5521 acactgctga cattctgttt ttccagtttc tgcagttgca ctttgacatc gtctgtcaaa
     5581 cccacataga agcctggaat atccagcacg ccgactttct ctttaccgac ggtcttcacc
     5641 gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt tacagtacgc
     5701 gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc cttaggccct
     5761 ttaattaagg ccaccacatc atcaagacgc cagccaatca cgtcaaccat cggcttgcct
     5821 gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttgctctt cgctgccgga
     5881 cctcctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag cactgcgcca
     5941 ataccttcca gcgacaaact catttcagtg ttgaactgtt cggtattacg cggggaaaga
     6001 tagttggtat gcgggtcgat ttcacgtgca aacgccgtca ttgccagcga gaaaacatct
     6061 tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgagt cagggtttca
     6121 cgaatttcct tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa tttgacttta
     6181 ctgtcccaca gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt gctgcggtca
     6241 aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga caaagcgtac
     6301 tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa aacgtcgagt
     6361 ttacctgaac gcagttcatc gcctaactca gttttctttt tcgcgaactg ttcaacatcg
     6421 cttgccagca gcacattatg gctgtaatcg agcagattca ggtagcggtc aaagattttg
     6481 gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa gcgcgacgtt
     6541 acgcgctcac tcaccgtcgc atgctgcgtc tcttccttta ataccggaat ttgatcagca
     6601 cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc agctaacgcg
     6661 gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca ccaggtgttc
     6721 tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt ctttggtgat
     6781 ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg cttgtccgac
     6841 agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg cttttactgt
     6901 ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc cttctttgcg
     6961 gcgtggcgtg gtcgggcgtg gcttgcgttc gcggcgcggt gcgtcttctt tctcaccagc
     7021 agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct gaacacgcgc
     7081 tttcgcttct tcaagctgct tgcgagcatg ctcgacatgt tgctcgtcca gctcaccgca
     7141 tgggttgccg tcaagatcaa cacgcgttgc gccggattta acaccgtaaa gataacgcca
     7201 gctcgaagtg tagagacgta aagcggatcg caattgcgtt ttgctcaggt tcatttcccc
     7261 ggcaacacga tcgaccaaat cctgaaaaat accgattttc agcggacgcg cttcaccttc
     7321 cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt tactgctatt
     7381 caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc aacaagcgca
     7441 ggcatgaaca ggcgtcatta taatgacgct atcagtaaat gctacgttat ccgttgatta
     7501 tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt tttcaagctg
     7561 tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgaccga agacggtact
     7621 atcgatgtcg agaacaccaa taatctgatt tttcaccacc agtggcagaa caatttcaga
     7681 attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct cgatacgctg
     7741 cacttgattg cgggcaaccg cagtgccgca tacgccgcgc ccaacgggta tccggacaca
     7801 ggcaattttg ccctgaaatg gtccgagcac cagtgtatcg tcctcaagca aataaaaacc
     7861 tgcccagttt acgtcagtga gacgctcata taacaacgca ctggtgttcg caagcgttgc
     7921 cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat ccgcgtaaaa
     7981 ttctgctttg ttcattatat aatcacttgg ttgtcttacc tggaactgcc agcctattaa
     8041 aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa gattaatgtt
     8101 aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac gccgacaaag
     8161 aagataacag tgagggctat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
     8221 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
     8281 cgctgtcagg caaaaattcg tgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
     8341 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
     8401 ctgttaggca ttcgtattga cgccaacgtt atgcaaggca tctggcaaat gaccaaacag
     8461 ggcgatgcga taacgggggc gatggtcttt ttctgcgtta tcggtgcccc cctcattctg
     8521 gtgacctcca tagcttattt atggtttggt aaccgattgg gaatgaattt acgcccggta
     8581 ctgctgatgc ttgagcggct gaaagagtgg gtgatgttgg atatctacct ggtcggcatt
     8641 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggggt cggcttgttc
     8701 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaagaa
     8761 ctgtgggagc gattttatcc gcagcgcccc gctacgcgaa gggacgagaa actccgtgtc
     8821 tgtcttgggt gccattttac cggctatcct gatcagcgtg gtcgctgccc gcgttgccat
     8881 atcccgctac tcctgcgtcg ccgtcatagt ctgcaaaaat gctgggcggc gctgttagcg
     8941 tcaatcgttt tgttgttacc tgccaacctg ttgcctatct ctatcattta tctgaatggt
     9001 ggtcggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag caacattgca
     9061 gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
     9121 ttcactttac tgttgagtat tcattttaaa tgccagcaag gtttacgcac acgcattctg
     9181 ttactgcgga tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct
     9241 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
     9301 ttttatttcg gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc
     9361 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
     9421 aaataaacgc cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgctgg
     9481 ttggctgatt tgggacagtt atcaggaccg gggcaatact gtcaccatcg actttatgtc
     9541 ggcggatggt attgttccag gccgtacgcc tgttcgttat cagggcgttg aagttggaac
     9601 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
     9661 gtccgatatg aaagatgcgc tgcgcgaaga gacacagttc tggttggtga cgccaaaggc
     9721 ctcgttggca ggtgtctccg ggctggacgc cctcgtcggt ggtaattata tcggcatgat
     9781 gccgggtaaa ggtaaagagc aggatcactt tgtcgcactc gacacccaac cgaaatatcg
     9841 gctggacaat ggcgatctga tgatccacct gcaagccccc gatctcggtt cactgagcag
     9901 tggttcattg gtctatttcc gcaagatccc ggtgggtaaa gtctacgact atgccatcaa
     9961 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
    10021 gaaaaaaggt agccgtttct ggaacgtttc cggcgttgat gccaacgtca gtatcagtgg
    10081 cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg ccttcgattc
    10141 acctgaagag tcgaaacctg ccgaagcgga agataccttt ggtctgtatg aagacctggc
    10201 ccatagccaa cgtggcgtaa taataaaact ggaactgcca ggcggtgccg gattaaccgc
    10261 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
    10321 aaatcctggt ggtaaagtca ctggggaaat gaccgttgat cccagcgtcg tcaccctgct
    10381 tcgtgaaaat acccgcatcg aattacgtaa tccaaaatta tcccttagcg atgctaatct
    10441 cagtgccctg ctgaccggca aaaccttcga actggtgccc ggcgatggcg agccacgtaa
    10501 ggagttcgtg gttgtgccag gcgaaaaagc cctgctgcaa gagccggatg ttctgacact
    10561 gaccctgaca gcaccagaaa gttacggtat tgatgctggt cagccgctca ttcttcacgg
    10621 cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt
    10681 cgccatcgag cctcagcatc gcgaactggt aaaaggcgat agcaaatttg tcgtcaacag
    10741 ccgtgtcgac gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga
    10801 atggatcaat ggcggaatac gtattctgcc gggcgataaa ggcgagatga aagccagcta
    10861 tccactgtat gccaatctgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac
    10921 aaccgtgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctgta
    10981 ccgtaaattt gaagttggtg aagttattac tgtgcgtccg cgagctaacg cgtttgatat
    11041 cgatctgcat attaaaccgg agtatcgcaa ccttctgacc agcaacagcg tgttctgggc
    11101 agaaggtggg gcgaaagttc agctgaatgg tagtggcctg accgtacagg catccccgct
    11161 ctccagagca ttaaaggggg ctattagctt cgataacctc agcggtgcca gcgccagtca
    11221 gcgtaaaggc gacaagcgta ttctgtatgc ttccgaaaca gcggcccgtg cggttggcgg
    11281 gcaaattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta
    11341 tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcgc gcaatgaagt
    11401 acaggcaaag gcggtgctct atccggaata tgtccagacc tttgctcgcg gtggtacgcg
    11461 cttctcagtg gtcacaccgc aaatttcggc agctggcgtt gagcatcttg atactatcct
    11521 ccagccgtat atcaacgtcg aaccaggccg gggcaatcct cgccgcgact ttgaattaca
    11581 agaggccacc attactgatt cgcgttacct ggatggctta agcattattg ttgaagcgcc
    11641 ggaagccggt tcgttaggta tcggtacacc tgtgctgttc cgtggtctgg aagtaggtac
    11701 ggttaccgga atgacgctgg ggacattgtc agatcgcgtg atgattgcga tgcgcatcag
    11761 taaacgctat caacacctgg tgcgtaacaa ttccgtcttc tggttggcat cgggttacag
    11821 tctggacttt ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc agtttatccg
    11881 tggcggcatc gccttcgcca cgcctccggg tacgccactg gcaccgaaag cccaggaagg
    11941 caagcacttc ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg gaactgcgct
    12001 tcccaaataa tgcccactgc tccggcgtgc ctgcgccgga gcgtttatgc taaactgcac
    12061 gcctgttttt ttgccagtgg tatatgctcg tggcccaaca caccgtttat ttcccggacg
    12121 cctttctgac acaaatgcgc gaagcgatgc cttcgacgct ctcttttgat gattttcttg
    12181 ccgcctgtca gcgcccgttg cgccgcagca ttcgcgttaa tacgctgaaa acctccgttg
    12241 ctgatttcct gcaattaacc gctccttatg gctggacgct tacgccaatt ccgtggtgtg
    12301 aagaaggttt ctggattgaa cgcgacaatg aagatgcatt gccgttgggt agtaccgccg
    12361 agcatttaag cggcctgttt tatattcagg aagccagttc aatgttgcct gttgccgcct
    12421 tgtttgctga cggtaatgca ccacagcggg tgatggatgt cgctgctgca ccaggttcca
    12481 aaacgacgca aattgccgcg cggatgaata acgaaggggc aatccttgcc aatgagtttt
    12541 ccgccagtcg ggtaaaagtg ttacatgcca atatcagccg ctgtggcatc agtaatgttg
    12601 cgctcacgca ttttgatggc cgcgtatttg gtgcggcagt gccagaaatg ttcgatgcca
    12661 ttttgctgga cgctccctgc tccggcgaag gcgtggtgcg taaagatccc gatgcgctaa
    12721 aaaactggtc accagaaagc aatcaggaaa tcgcagcgac ccaacgggag ctgatcgaca
    12781 gcgcctttca tgcattacgt cctggcggta cgctggttta ctcgacctgt accttaaacc
    12841 gggaagaaaa cgaagccgtt tgcctgtggc tgaaagagac ttaccccgac gcagtagagt
    12901 ttttaccgct tggcgatctc ttccctggtg caaacaaggc gctgaccgag gaaggctttt
    12961 tgcatgtttt cccacaaatt tacgactgcg aaggcttctt cgttgctcgt ctgcgtaaaa
    13021 ctcaggcgat ccccgtctta cccgccccaa aatacaaagt gggcaatttc ccgtttagcc
    13081 cagtgaaaga tcgcgaagcc ggtcaaattc gtcaggcggc tgcaagtgtt ggcttaaact
    13141 gggatgaaaa cctgcgactc tggcagcgtg acaaagaact gtggttgttc ccggtgggca
    13201 ttgaagccct gatcggtaaa gtccgatttt ctcgcttggg gattaaactt gccgaaacgc
    13261 acaacaaagg ttatcgctgg cagcatgagg ccgttattgc ccttgcctcc cccgacaatg
    13321 tgaacgcttt tgaactgaca ccgcaggaag cggaggagtg gtatcgcggg cgcgatgtat
    13381 acccgcatgc cgcgccagtg gcggatgatg tattggttac tttccagcat cagccgattg
    13441 gtttagccaa acggattggt tcacgactga aaaacagcta cccgcgtgaa ctggtgcgcg
    13501 acgggaaact ttttaccggt aacgcctgac agcgcacaaa aaagcgcact ttttgactgg
    13561 cacattcgac tgcctcaact aggctgaaaa atggtgcgat cggactggtc gtaccacaac
    13621 cggcagctaa atggagagca cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat
    13681 cgacgacggc gaattacacg gcgaatcgcc gggtgatcga acgttaacca ttccttgtaa
    13741 atcagacccc gatttatgta tgcaactgga tgcctgggat gctgaaacca gtatccctgc
    13801 cctgctaaat ggcgaacact ctgtccttta ccgtacccgt tacgatcaac aatctgatgc
    13861 ctggattatg cgtcttgcct gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc
    13921 gggttattcc cccgttgtaa aatctctcct aaacttaacg gtacggcacc acacttcggg
    13981 gatgaaatgt tcgcgctggt actttttgtt tgctacctgg atggcggttg tgaagatatt
    14041 gttgtggatg tctacaacac ggaacagcag tgtctttatt ctatgagcga tcaacggatc
    14101 cgccatggcg gttgttttcc gattgaggat tttatagatg gtttctggcg acctgcacag
    14161 gagtacggtg atttttaatt attgcaattg cacaagagtc agttctcccc caaagacagc
    14221 accggtatca atataatgca ggttgccaat atccacgcga tggcgcaaag gtgtatgacc
    14281 aaaccagaaa tgatcagcac ctgcaattcc ctgccctttt tggcgttcac ctaatcgcga
    14341 gcggctccac aagacctgat gcaaatcgac gtccttttgc cattcataaa catcatctgg
    14401 ataatcggca tgagcaataa catgtttgcc ggtgcggctg tgtacttcaa gaataaaggg
    14461 caaatgctga catttttcca gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc
    14521 aataaaccag tcgccgccat tcatcaacca taaagacatc tgccgggatg ccagcgcatc
    14581 catcgccatc tgttcatgat tgcctcttac cgcacgaacc caatgttgtt ccagtaactg
    14641 caggcaacgt aaactttgcg gcccacgatc gataacgtct cccactgaga taagtaaatc
    14701 tcgccacgga tcaaaacgac aatgccataa tttgcggcgc aattgctcaa gacaaccgtg
    14761 tatatcgcct gaaagccaga tatgtcgcca ttgatgaccc gcaattctct gataaacggg
    14821 cgcaggctgt ttcatcaata ttttcctccc gcgctaaaga tcacataatc ttaacaagaa
    14881 tgttaaaaaa cgctggactc aaacagtaga gtgtgtgtta tggttgacta taaagtcagc
    14941 gaagggaatg cttctggctt ttaacagata aaaagagacc gaacacgatt cctgtattcg
    15001 gtccagggaa atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct
    15061 tagttgcctt gctctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa
    15121 tggtcaataa aaagcgtggt ggtcatcagc ttaaatatta aaaaccgccc gttctggtga
    15181 aagaactgag gcggtttttt attggaaatc aaaaggctat tttaggtaat taacagagtt
    15241 tttcagctcg ctctataaac ggtgccagac tcattttttc gccgggattg ttaggatcat
    15301 caatctgaat caccgaaatg ggttgggctt tagtcttccc actggcaact tccttttgtg
    15361 cgatatcgtt taaaggatac tgcacgagag tacttgggtt aatgacatac aaagcattac
    15421 ccggtcggca agtcagcatc acctcttcgc gattaaacgc ccatttgtct ttacctactt
    15481 caaaacggct gacggtaatc acctgcggtg ccgccagcgc agctgcagaa ctggtgagta
    15541 acagaaacgc cagaatactt tttttcatca ttttcgtaat ccatcaaaaa ggttcgagag
    15601 tcgcaagcag gctgatgatc accagcacta ccgcacctat cgcccattcg agtttagtca
    15661 tccagacaaa ccagggagcc gcacgatctt catcctgcct catgcgcggt acaagaacat
    15721 aacgattagc cagcgcgatg accaccataa tcatcaccag gatggctttg agcaataaga
    15781 gttggcccca gtgagtcgta agcgtggggg gaaatccggt aatcaacaat gcattaagca
    15841 cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca ccaggaaaaa cgcatcagcg
    15901 cctgaatagc ctggtgtcgc caacggcctt tgatgagttg catacaccag agcaccggca
    15961 gtaatccccc aaaccacgcc gccgcacaga tcaggtgaat tgcatgatta gtctgatgga
    16021 ttttcgccgt taccccttca ttcagcgtcg catgaccgac tcccgccagc agaataaatt
    16081 gcgcggtagt gagcataaat agcaaacgtg gcatattctg cggttgcata agggcgacga
    16141 tcaatgttac gagggcgaga acaatttgcc ataaccagac gccaccaaac tgcgtttgca
    16201 aaacagccag ccaaatgttt ggcgaaaata catctgacca tcccgttccc atcaacccgc
    16261 cctgaacggc gagcattgca gtggcactga tcaaactcca gacggcagcg tgctgttgta
    16321 aacgcagaaa acgcttcgtc agcaagcgac gaatcgttaa gggggccagc caggcaccgt
    16381 acattgcgaa gccaaaaacc agcatcagcg aggtgaaatg gataaatcgc agcgcgatcc
    16441 aggtaaacgc cagcatgatt tatttcacgc taaaggtgta atgtcctttc gttttatgcc
    16501 catccaccga aacaacatgc cagtcaacgg tgtaggttcc gggtttcagc gaatcggcca
    16561 gtggaacaat cagttgcttt tggtcctgct cattacgctt cgccggtaat gttttaatat
    16621 tttcgttttt tggccccgtg atttttgcgc cactgaatcc tgtttcaacg ccttccgaga
    16681 agtttaaagt gattgcctgc ggagctgctg tcacttgtgc atttgccgca ggatactgat
    16741 gcgttaaatg cgcatgtgcc cagacggaag gtgtcaccaa cgaagtggtc aggattgcca
    16801 gcgcgtagcg aagggagcgt gcagttgaag ccatattatc tattcctttt tgtaataact
    16861 tttttacaga gcataacctt gtctaatgtc tgagtcgagg atcatcaatt ccggcttgcc
    16921 atcaaggctc actcttagta acttttgccc gcgaatgatg aggagattaa gaatgctgaa
    16981 gaatctggct aaactggatc aaacagaaat ggataaagtg aatgtcgatt tggcggcggc
    17041 aggggtggca tttaaagaac gctacaatat gccagtgatc gctgaagcgg ttgaacgtga
    17101 acagcctgaa catttgcgca gctggtttcg cgagcggctt atcgcccacc gtttggcttc
    17161 ggtcaatctg tcacgtttac cttacgagcc caaacttaaa taaaacttat acatagttac
    17221 actttcttac ataatgcctg ctaaattatg agtattttct aaaccgcccc cataatttgc
    17281 agtcattttg aaaaggaagt cattatgtcg ttctggaaag ttgcagcagc gcaatatgag
    17341 cctcgtaaaa cctcactcac cgaacaggta gctcatcatc tggagtttgt gagggccgcc
    17401 gccaggcaac aatgtcagct gttagttttt ccatcgcttt ctttactggg gtgtgattat
    17461 tcgcgacgtg cccttcctgc cccacccgat ctctcgctat tagacccgct ttgttatgcc
    17521 gcaacgacct ggcgaatgac catcattgcc ggccttcccg ttgaatataa cgatcgcttt
    17581 attcgtggaa ttgcggtatt cgcaccgtgg cgaaaaacgc cagggatcta ccatcagagt
    17641 tatggtgcat gtctgggccg tcgttccaga accatcacgg tggtcgatga acaaccgcaa
    17701 ggtatggata tggacccgac ctgttcactg tttacgacag ggcaatgtct gggagaaccc
    17761 gacctgctgg cgtcggcccg tcgcttacaa tttttttcac atcagtactc gattgctgtg
    17821 ctgatggcaa atgcccgtgg taacagtgca ctatgggatg aacatggtcg tctcatcgtt
    17881 cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt catcacaggg ttggcaaggc
    17941 gatatcattc cattacgcta ggctttttcg gcctggagca tgccatgttg cgcattatcg
    18001 atacagaaac ctgcggtttg cagggaggga tcgttgagat tgcctctgtt gatgtcattg
    18061 acggaaaaat cgtcaatccc atgagccacc tggtgcgtcc cgatcgtcct attagtccac
    18121 aggcgatggc gattcatcgc atcaccgaag ccatggtcgc cgataaaccg tggattgaag
    18181 atgtgatccc gcactattac ggtagtgaat ggtatgtcgc gcataacgcc agctttgacc
    18241 gccgcgtact gcctgaaatg cccggtgagt ggatttgcac tatgaaactg gcccgtcgtt
    18301 tgtggccagg gatcaagtac agcaatatgg cgttatataa aacacggaag ctcaatgtac
    18361 agacgccgcc gggcctgcat catcaccgcg cgttgtatga ctgttatatc accgctgcgt
    18421 tgcttatcga tattatgaac acctccggct ggacggcaga acagatggcc gatatcaccg
    18481 gacgtccatc gttgatgacg actttcacct ttggcaaata ccgtggcaaa gcggtttccg
    18541 acgttgccga acgcgatccg ggctatctgc gctggttatt taataacctg gacagcatga
    18601 gcccggagct gcgtttaaca ctgaaacatt atctggaaaa tacttaatcc gcaggcgtag
    18661 cgggtaatgt tccctgcgcc agcgcgacca gaaaagcata ttccatcgct acgccttcgt
    18721 acgatttaaa gcgaccagat ttaccgccat ggcctgagtc catgtcggta cagagcaata
    18781 aaagatggtt atcggttttc agctcgcgca atttagcgac ccattttgcc ggttcccaat
    18841 attgcacctg agaatcgtgt aaaccggtcg ttaccagtaa atgcggataa gcctgtgcgg
    18901 tgacgttgtc atacgggctg tagcttttca tatactcgta atattgcgga tcctgcggat
    18961 tcccccactc ttcaaattcg ccggtggtaa gaggaattga ttcatccagc attgttgtta
    19021 caacatcaac aaacggcacc tgggcgataa cgccgtggaa caattccggg cgttgattaa
    19081 tcgcaacgcc catcaacata ccccccgcac tcccgcccat cgcataacag agcgaaggag
    19141 agccatagcc cagttttaac aatgcatcgc aggcatcaag ataatcatta aacgtatttt
    19201 tcttcttcag aaatttaccg tcctcgtacc attgttgccc cagctcaccg ccgccacgaa
    19261 catggacaat ggcgtagaca aatccacgat ccaacaaact caagcggcta aaactgaaat
    19321 cggcatcaat acttgcgccg taagaaccgt agccatacac cagcagcggg ttgtgtcctt
    19381 tgcgaaaatg tttgcgatga tagaccagcg aaaccggaac ttcgacgcca tcacgggcga
    19441 ctatccacag gtgttcactg cggtaattcg ccgcatcaaa accaggaact tccgtttgtt
    19501 ttaatacacg acgctcaccg gtatccatat ccagttcaaa caaagtatct ggtgtggtca
    19561 tggacgaata accataacgc aatcgcgcgg tttcaggttc tggattgtag gcaatccagg
    19621 tcacataggc cggatcatca aaggcaatgc caatgacttc ccgggtcttg cggttaattt
    19681 gccgcaaact ggttaacccg cgctgacgct cttcaaccac cagccagtcg gtaaacagcg
    19741 taaacccttc cagcataatg ttatcacgtg gcggaattaa ctcttcccac tgttgctcat
    19801 cacgcatacg ggtacggtat aagccaaagt ttttaccgtg gcggttggaa cgcagataaa
    19861 accgatgctg gtagtgatca aggctgtatt cgtgatcttt gcggcgcggc agaaaaacaa
    19921 acggctcggc atcggctatt tccgcgtcca gcaggcgaac ttcactggtg gtggcgctgg
    19981 ccagatgaat gactacatag tgcttcgagg tcgttttatg caggctgacg taataggtat
    20041 cgtctttttc ttcgtagatc agtttatcct gcgatgctgg cgtaccgatg gcgtgacgcc
    20101 agacctgata aggaagcagc gtcaccggat gcttgcgaac atagtagaaa gtccaggaat
    20161 catttgccca gacaaagctg gggtcaacgt tatccagcag ttccggatac cagttacctg
    20221 tttccagatt acgaaaacga atgccgtact ggcgtcggga aagaaaatct tctgccagtg
    20281 ccataatggt gttatcgggc gtaatcgcca ttccgcccat cgaataaaac tcactatgag
    20341 ccgcgcgctt gttggcatcg agcaatgttt cccactcatc ccactcttcg ctgaacgccg
    20401 attgacgctg gtagatagca tattcacagc ctggttcata aatatgccga tagcggtagc
    20461 cattttttat gtagggcgca gaaacttctc tttgcggaat gcggtcgatg atttccttta
    20521 agatgcgatc ctgcaaggct tgttgcgagg ccatcacccg atgaccgtaa ctattttctt
    20581 gttgcagata gtccaggact tccggctgag aacgcgtatc gtcccgcagc cagtagtaat
    20641 tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg aatgcgggcg gcttttggta
    20701 gcatgttatt gttctttctg gttgaaacat cttataaggg tggcaaaact caccggggat
    20761 gcaagcgaaa cagggtagtc attgcttaga tgatgacagg taatggcgcg gatatcgact
    20821 gttatgcaga cagagaaaat cagcctgttc aaatggctgt gcggttctgg ataacccgaa
    20881 atagtcaact tcaggctatc cagagagcgg aattactccg ccaaagtacg tttttgctgt
    20941 tcgagatcgc gttcaatgcc ttcacgaacg tcctggggga ttttcagcgc atcacccagt
    21001 gcattcaggt aactgcgttc cataaaatgg tcaatatcaa tagccgcgca gctcaggaaa
    21061 tagatttcca gcgcctcttc ttcattgcgg accccggtag ccaggcgttg tggatccagc
    21121 ggttgttcga ttgcctgctc aatgagtaca cgccccttct cttccacacc ggcttcacgc
    21181 aattgcaggt cgattgccgc acgttctttg gcatcaatat gaccatcact tttagcggca
    21241 aagaccagcg caaggatcaa acgttccgta cgctcatcca gcggcgtact ttgcgcgcca
    21301 aactgcggtt cgtcctgatg cgccgcgcga attttatctt tgtatttatt ccacagcacc
    21361 gtacccgcta ccgctccgcc gccaaccagt aacgcgtttg tgccatattt cgtaagaagt
    21421 ttacgtgctg atttatttgc gaccagcagc ccagccagtc cgcctaatgc gccaggcact
    21481 aaaagtttgc ccaatccctg atccgcagac gaggaggtag aagaacggct ttgcccaagc
    21541 agggattgca gttgatttaa ccagttagcc atatttgctc ctcaataacc atttttagtg
    21601 cctgtagcat agcggatgag gatgtcagaa aatgtatatc gagacaaaag atgcgcaaat
    21661 aagcggggcg gtaggccgtt attcaaagaa attcgcgcca ggtaaggtgt tcactgtata
    21721 taaggtgata agggtatttt aggtgaaagt agtttgacat tttgagtgga actgtttgat
    21781 gctgattgcc cggttcgacg ctactgcgtc ttctcaggcc tacgattgtg ttgcgttgta
    21841 gggcggacaa ggcacccgcg ccgcatccgc caacggtcaa tcgaattatc agaagccatt
    21901 aacgccgctg atattccgcc attcccgctt tgcagtccac gctgacctgg taatgaatat
    21961 cggcactttt accacgcacg gttagcggta ctgaccattt atcatctttc ccctgaatgt
    22021 cctgcaaact gacccatgcc acgggatcgg cctgaccgac aattttttga tcatcagccc
    22081 agcgcgccac gcgattttgt tggtaatcac gttttacgct cgcggcaatt ccggcagcat
    22141 ccagaccttc acactttggg aaagtgaccg acttgctggt ttcattattg gcagcgaaaa
    22201 ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc ccctcttttt ttcatgtttt
    22261 tctccatagc acaatgattc aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc
    22321 ttcaggccgc gtgtgaggca tcttcctctt cggattcagc accgacgacc tgtggggacg
    22381 gtaatttgcc ggttttcaga atggtgctca gcacatcttt ttgttctgcc agccatagcg
    22441 aaagggcttc acgttgctcg tcttccattt gcactggaga atttgtcagc caggtgtcaa
    22501 gcagatcggc cgtatcaagc atcttgtcat aagcatcggc ttcctttttg ctggtaaacg
    22561 acattttctc ttcgccctca cgaatgacta cgtatttaac ttcaaccgcc atttgcagcc
    22621 tctcataata actgtggttt tatacagtat atttcttttc agttgggaaa tcaacatcag
    22681 caataaagac acacgcaaac gttttcgttt atactgcgcg cggaattaat caggggatat
    22741 tcgttatgac gttattaggc actgcgctgc gtccggcagc aactcgcgtg atgttattag
    22801 gctccggtga actgggtaaa gaagtggcaa tcgagtgtca gcgtctcggc gtagaggtga
    22861 ttgccgtcga tcgctatgcc gacgcaccag ccatgcatgt cgcgcatcgc tcccatgtca
    22921 ttaatatgct tgatggtgat gcattacgcc gtgtggttga actggaaaaa ccacattata
    22981 tcgtgccgga gatcgaagct attgccaccg atatgctgat ccaacttgaa gaggaaggac
    23041 tgaatgttgt cccctgcgct cgcgcaacga aattaacgat gaatcgcgag ggtatccgtc
    23101 gcctggcggc agaagagctg cagctgccca cttccactta tcgttttgcc gatagcgaaa
    23161 accttttccg cgaagcggtt gccgccattg gctacccctg tattgttaaa ccggtgatga
    23221 gttcttccgg taaagggcag acgtttattc gttctgcaga gcaacttgct catgcatggg
    23281 agtacgctca gcaaggtggt cgcgccggag cgggccgcgt aattgttgaa ggcgtcgtta
    23341 agtttgactt cgaaattacc ctgctaaccg tcagtgcggt ggatggcgtc catttctgtg
    23401 caccggtagg tcatcgccag gaagatggcg actatcgtga atcctggcaa ccacagcaaa
    23461 tgagcccgct tgcccttgaa cgtgcgcagg agattgcccg taaagtggtg ctggcacttg
    23521 gcggttatgg gctgtttggt gtcgagctgt ttgtctgtgg tgatgaggtg attttcagtg
    23581 aagtctcccc tcgcccgcat gacaccggga tggtgacgct aatttctcag gatctctcgg
    23641 agtttgccct gcatgtacgt gccttcctcg gacttccggt tggcgggatc cgccagtatg
    23701 gtcctgctgc ttctgccgtt atcctgccgc aactgaccag tcagaatgtc acgtttgata
    23761 atgtgcagaa tgccgtaggc gcagatttgc agattcgttt atttggtaag ccggaaattg
    23821 atggcagccg tcgtctgggg gtagcactgg ctactgcaga gagtgttgtt gacgccattg
    23881 aacgcgcgaa gcacgccgcc ggacaggtaa aagtacaggg ttaaacccgg caaaaaaacg
    23941 ctacaaaaat gcccgatcct cgatcgggca ttttgacttt tacagcttag cgccttctac
    24001 agcttcacgc gccagcttag taatgcggtc gtaatcgccc gcttccagcg catctgccgg
    24061 aaccagccag gaaccaccga tgcacagcac gcttttcagc gccaggtagt cacggtagtt
    24121 agccggagaa ataccacctg tcgggcagaa acggacctgg gagaacggac ccgcgatcgc
    24181 ctgcagggct ttcacaccgc cgttagcttc agccgggaag aatttgaact ctttcaaacc
    24241 gtagtccata cccagcatca gttcggaaac agtgctgatc cccggaatca gaggaatagt
    24301 cccttcggta gcagctttca gcagcggctc ggtcagaccc gggctaattg caaactgtgc
    24361 acctgcttca gtgacttctg ccagctgctg tggattcagc accgtaccgg cacccacaat
    24421 cgcttcaggc acttctttgg cgatagcacg gatagcgtca actgcgcact cggtacgcag
    24481 agtcacttcc agaacgcgca ccccaccagc aaccaacgct tttgctatcg gcaccgcgtg
    24541 ttccagtttt tttaccacga taaccggtac aaccgggccg gtggtcagga ttgattctgc
    24601 acttgttttc cagtttttca tcagagtttt ctctcgcctg attacaaatt tgtcgtctta
    24661 aaaagtgata caggttgcgc cctgttcggc accggacagt ttttcacgca aggcgctgaa
    24721 taattcacgt cctgttccca cgcgtgacgc gctcaggtca ggaatgtgcg gttcgcgagc
    24781 agccagttcc gcttcgtcta ccagcagcgt cagttcgcct gtctgtccat tcacacgaat
    24841 gatgtccccg tcgcgcactt ttgccagcag cccgccatcg taggcctctg gtgttacgtg
    24901 gatagctgac ggcactttac ctgaagcgcc ggagagtcgt ccatcggtaa ctaacgcaat
    24961 tttgaaacac cggtccaata atacaccaag tggcggcatg agtttatgta attctggcat
    25021 tccgttcgct tttggcccct gatgacggac aacaacgaca caatcgcggt ccagtaaacc
    25081 cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa acaaccgctg gcgcttcaat
    25141 cacctggttc tctaccggca cggcagaggt tttcataacc gctcgcccca ggttaccgct
    25201 taacactttt gtcccaccat gatgagagaa aggttgttcg aaggacgcga tcacattgtt
    25261 gtcgagtgat ttttctgccc cttcccgcca gtccagttca ccattattca gccatggttc
    25321 aagggtataa cgagacagac caaagcctgc caccgtattg acatcttcat gcagcaggcc
    25381 tgctttgagc agttcacgta ccagaaccgg tacgccacct gccgcctgga agtggttaat
    25441 atcggccgga ccgttcgggt agagacgtgc catcagcggt acaacatcag aaaggtcaga
    25501 gaagtcatcc cagttaatct gaataccggc tgcgcgcgcc atcgccacta ggtgcatggt
    25561 gtggttagtg gaaccaccgg tcgccagcag tgcaacgata ccgttcacca ccactttctc
    25621 atcgatcatc ttaccgatcg gcatccattc attaccatta ccggtcatgc gcgtaacctg
    25681 gcgcgcagct gcagcggtca aagcatcgcg cagcggagaa tccggatgaa caaaagaaga
    25741 gcctggcaac tgcatcccca taaactccac caccatctgg ttggtgttgg cagtaccgta
    25801 gaaagtacat gttcccggcg catgataaga cgcggcttct gactccagta atgccatgcg
    25861 gtccacttta ccttcggcat aaagctggcg aatacgcact ttttctttat ttggcaaacc
    25921 gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa tgaccaaacg acagggctgc
    25981 catggtcaga cctgggacaa tcttgtcgca cacaccgaga aacagagcac catcaaacat
    26041 gttatgggac agccccaccg ccgcagacat cgcaatcact tcgcggctta acagcgacaa
    26101 ttccattcca tcctgcccct gggtgacacc atcacacatc gccggaacac cgcccgcaac
    26161 ctgaccaacc gcattcgctt catgcagggc tttacgaatg atttctggat agtgttcata
    26221 aggctggtga gcggagagca tgtcgttata ggaggtgatg atggcgatat tgttacgcaa
    26281 catgcttttc aaagaggctt tgtcttctgg ctggcaggca gcgaaaccgt gtgccaggtt
    26341 accgcatgcc aactgcgaac gatgaacggt cgaagttttc gcttgttcta tccgggcgag
    26401 gtaagcagag cgagtctcgc gcgaacgttc aatgattcga tttgttacgc gtaacaattg
    26461 tgggttcata aaggctcctg aaattgagtt gtcagagcag gatgattcac aacgcgtttc
    26521 atacatggaa attatgattg aaacgcctgt aaccggagct catagggcaa aaacgtttca
    26581 gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg aggcctgaaa gtgtaaaaat
    26641 tgttctacaa tctgtgcaag atcatgttac cggtaaaata accataaagg atgagcgcag
    26701 atattactca aactcattcc aggaacgacc atcacgggta atcatcgcca ctgaggcaac
    26761 gggtccccag gttccggcct gatacggttt cggcgcatca ttgtccatcg cccacgcctc
    26821 agtaatggag tctacccatt tccaggcttc ttccacttcg tcgcgacgta caaacagtgc
    26881 ctgaatacca cgcatggttt ccagcagcaa acgttcatag gcatccgcca gatgcgtctg
    26941 attaaaggtt tctgaatagc tcagatccag cttggtgatt tgcaggttat gtttgtggtc
    27001 aaggccagga actttattca gtacctggat atccacgcct tcatcaggtt gcagacggat
    27061 agtcagttta ttttgcggta gatcctgcca cgactcttta aacagattca gttcaggtgt
    27121 tttgaaatag accacgactt cagaacattt ggtcggcaga cgtttaccag tacgcaggta
    27181 gaatggcaca ccggcccagc gccagttatc aatgtcgacg cggatcgcca cgaaggtttc
    27241 tgtattgctg ctcttgttcg cgccctcttc ttccagatag cccggcactt ttttgccctg
    27301 agcgaagccc gcagtatatt gcccgcgtac ggttttttcg cgcacattgg agcggtcgat
    27361 gcggcgcaga gacttcagta ctttcacttt ttcatcgcgg atgctgtctg cgctcaggtc
    27421 agacggcgga gacatcgcaa tcatgcaaag aatttgcagc aggtggttct ggatcatgtc
    27481 gcgcatctga ccggctttat caaaatagcc ccagcgccct tcgatcccca cttcttctgc
    27541 cacggtaatc tcaacatgat caatggtgcg attgtcccag ttgttcacaa acagagagtt
    27601 agcaaaacgc agcgccaaca ggttcagcac tgtttcttta ccaagatagt ggtcgatacg
    27661 gtaaacctgg cactcctcga agtattcgcc aacctgatcg ttgatttcct gcgaggtcgc
    27721 cagcgacgtc cccagcggtt tttccatgac tacgcgtgcc ggtttagcat tcagttttgc
    27781 ctcaccaagc cctttgcaaa ttgcgccaaa agtgctgggc ggcatggcaa agtagttaat
    27841 ggtgatacga tttttttgat ccagcatcgc gccgagacgg ctgaatgcag cagtgtcatt
    27901 gacatcaaga ttacaaaaat ccagacgtgc actcagggtg tcccataaac cttcatcaat
    27961 ggtttctttc atgaaagttt cgagcgcctc gcggacaact ttggtataag ccgctttatc
    28021 ccagtcagca cgccctacgc cgataatccg ggtgtccggg ttgagctgac cggctttttc
    28081 cagttgatac agggaaggca gcaatttacg acgcgcaagg tcgcctttcg cgccgaaaat
    28141 gaccaggtca caggcctggg ctgtttgcgt taccgccatg tcattctcct taagttaact
    28201 aacccggtac ttaagccagg gtatacttgt aattttctta cggtgcactg tactgctttt
    28261 acgagcttgc gaaaactgta aacgcttatc cacccgtgcg attacggtaa aagcgcgcaa
    28321 agtgcggcaa aactgataaa aaaatcatcg ttttccattg ggtaaaaatc tgacactgat
    28381 catgttatga aaaaaaataa caactttttt atctgctttt gtcattaaca cagcacacag
    28441 gcgtaatatc tgacaaaact gcatttcgat ttctttcaga gcggaaatcg tcattacccg
    28501 tgagtctctt tacatcatga atatgctgga aaaaatccag tctcagctgg aacatttgag
    28561 caaatctgag cgcaaagttg ccgaggtcat tctggcttcg cccgataacg cgatccattc
    28621 gagtattgct gctctggcgc tggaagccaa tgttagcgaa ccgacggtga atcgtttctg
    28681 tcgcagcatg gacacacgcg gttttcctga ttttaaactt catctggcac aaagtctggc
    28741 gaatggcact ccctatgtta atcgcaacgt caatgaagat gacagcgttg aatcatacac
    28801 ggggaaaata tttgagtccg caatggcaac gctcgatcat gtccgtcatt cactggataa
    28861 atctgccatc aaccgcgccg tcgacttgct cactcaggca aaaaaaatcg cctttttcgg
    28921 attaggttct tcagccgccg ttgcccacga tgcgatgaat aagttctttc gttttaatgt
    28981 tccggtggtg tactccgatg atatcgtgct gcaacgcatg agttgtatga attgtagcga
    29041 cggagacgtg gtggtgctga tttctcacac gggaagaaca aaaaatctgg tcgagctggc
    29101 gcagctggca cgcgaaaacg acgctatggt aattgccctc acctctgcgg gtaccccact
    29161 cgcccgggaa gcaacgctgg caattaccct cgacgtaccg gaagatactg acatttatat
    29221 gcccatggtt tctcgacttg cacagctgac cgtgatagat gtgctggcga caggatttac
    29281 tttgcggcgc ggtacaaaat tcagagataa cttgaagcgg gtcaaggaag cgctgaagga
    29341 atcgcgtttt gataagcagt tacttaattt aagtgacgat cgctaaaaac gactgtcact
    29401 gtcctaatct tatacgacat ccgaatgaga ttaatttatc gccatcacgg cgttatttca
    29461 ttcggatttc atgttcaagc aacacctggt tgtttcagtc aacggagtat tacatgtcca
    29521 gaaggcttcg cagaactaaa atcgttacca cgttaggccc ggcaacagat cgcgataata
    29581 accttgaaaa agttatcgcg gcgggtgcca acgttgtacg tatgaatttt tctcacggct
    29641 cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga gattgccgca aaactggggc
    29701 gtcatgtggc tattctgggt gacctccagg ggcccaaaat ccgtgtatcc acctttaaag
    29761 aaggcaaagt tttcctcaat attggggata aattcctgct cgacgccaac ctgggtaaag
    29821 gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg cctgcctgct gacgttgtgc
    29881 ctggtgacat cctgctgctg gacgatggtc gcgtccagtt aaaagtactg gaagtccagg
    29941 gcatgaaagt gttcaccgaa gtgaccgtcg gtggtcccct ctccaacaat aaaggtatca
    30001 acaaacttgg cggcggtttg tcggctgaag cgctgaccga aaaagacaaa gcagacatta
    30061 agactgcggc gttgattggc gtagattacc tggctgtctc cttcccacgc tgcggcgaag
    30121 atctgaacta tgcccgtcgt ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca
    30181 aggttgaacg tgcggaagcc gtttgcagcc aggaggcaat ggatgacatc atcctcgcct
    30241 ctgacgtggt aatggttgct cgcggcgacc tcggtgtgga aattggcgat ccggaactgg
    30301 tcggcattca gaaagcgttg atccgtcgtg cacgtcagct aaaccgcgcg gtaatcacgg
    30361 cgacccagat gatggaatca atgattacta acccgatgcc gacgcgtgca gaagtcatgg
    30421 acgtagcaaa cgccgttctg gatggtactg acgctgtgat gctgtcggca gaaactgctg
    30481 ctggtcaata cccgtcagaa actgttgcag ccatggcgcg cgtttgcctg ggggcggaaa
    30541 aaatcccgag catcaacgtt tctaaacacc gtctggacgt tcagttcgac aatgtggaag
    30601 aagccattgc catgtcagca atgtacgcgg ctaaccacct gaaaggcgtt acggcgatca
    30661 tcaccatgac cgaatcgggt cgtaccgcgc tgatgacctc ccgtatcagc tctggtctgc
    30721 caattttcgc catgtcacga catgaacgta cgctgaacct gactgctctc tatcgcggcg
    30781 ttacgccggt gcactttgat agcgctaatg acggcgtagc agctgccagc gaagcggtta
    30841 atctgctgcg tgataaaggt tacttgatgt ctggtgacct ggtgattgtc acccagggcg
    30901 acgtgatgag taccgtgggt tctactaata ccacgcgtat tttaacggta gagtaagtac
    30961 gttgccggat gcggcgaaaa cgcctcatcc ggcctacagt tcaatgataa ttcaacagat
    31021 ttcgaatctt ctgaagcaaa cttgaactta tcatcagtcg aaggcctctc ctcgcgagag
    31081 gcatttttta tttgatggga taaagatctt tgcgcttata cggttggatt tcgcccggtt
    31141 tgcgagtttt cagcaatttt aatatccagg tgtattgttc tggtcgcgga ccaacaaaaa
    31201 tctcgacttc ttcattcatc cgccgcgcaa tcgtatgatc atccgcctct aacagatcat
    31261 ccatcggtgg gcgcacctga atcgtcagac gatgcgtctt gccatcataa atcggaaaca
    31321 gcggtacaac gcgcgcacgg cacactttca tcaaacgacc aatcgcgggc aacgtcgctt
    31381 tataggtggc aaagaaatca acaaattcgc tgtgttctgg gccatgatcc tgatcgggta
    31441 aataatatcc ccagtaaccc tgacgtaccg actggatgaa tggtttaata ccatcatttc
    31501 tcgcatgcag acgaccacca aagcgacggc gcaccgtgtt ccagacataa tcaaaaaccg
    31561 ggttgccctg attatggaac atcgctgcca ttttctgccc ttgcgaggcc atcagcatgg
    31621 caggaatatc gacggcccaa ccgtgcggca ccagaaaaat cactttctcg ttattacgcc
    31681 gcatctcttc gatgatctcc agcccttgcc agtcaacgcg cggctgaatt ttctccggcc
    31741 cgcgtattgc caactcagcc atcattacca tcgcttgcgg cgcggtggca aacatctcat
    31801 ctacaatcgc ttcgcgttca gcttcactac gttctggaaa gcagagcgac agattgatta
    31861 acgcacgacg gcgtgagctt tttcccagtc gtccggcaaa acgtcccagc cgtgccagaa
    31921 tgggatcacg gaactttggc ggcgttaaag cgatacccgc catcgctgct acgcccagcc
    31981 atgctcccca gtagcgcggg tggcgaaagg atttatcaaa ctcaggaatg tattcgctat
    32041 tatttttttt cgtttccatg cttttccagt ttcggataag gcaaaaatca atctggtgat
    32101 agtgtagcgg cgcaacttgc cccgcaccaa ataaaaaagc cggtactgac tgcgtaccgg
    32161 ctgcgaatgg atgttaatta atcaaaccgt agctgcggca caatctcttt ggcctgtgcc
    32221 aggaattcgc gacgatcgga gccggtcagc ccttcggtac gcggcagttt tgccgtcagc
    32281 gggtttacgg cctgctggtt tatccatact tcatagtgca gatgcggccc ggttgaacgt
    32341 ccggtattac cggaaagcgc gatacggtcg ccacgtttca ccttctgtcc cggtttcacc
    32401 aggatcttgc gcaagtgcat ataacgcgtg gtgtagctgc gaccatgacg aatagccaca
    32461 taataacctg ctgcgccact acgtttggca accaccactt caccgtcacc cactgaaagc
    32521 actggcgtac cttgtggcat ggcaaaatca acacctctgt gtggcgcaac gcgaccggtc
    32581 accggattag tacgacgcgg gttaaagtta gatgagatac ggaactgttt cgccgtcggg
    32641 aatcgcaaga atcctttcgc cagaccagta ccgttacgat catagaattt gccatcttca
    32701 gcgcggattg cataataatc tttaccttct gaacgcaaac gtacgcccag cagctggctt
    32761 tgctcacgtt taccatcaag catttctcgt gacattaaca ccgcaaattc atcgcctttt
    32821 ttcagtttgc ggaaatccat ttgccactgc atggctttaa tcactgcgct cacttcggcg
    32881 ctggttaaac cggcgtttct ggcgctggca acaaagcttc ccccgacggt acctttcagc
    32941 agattgttga cccactctcc ttgctgcatt tcgctggtca ttttaaaacc gttagcggca
    33001 gtacggtcat aggttcgggt ttcacgacga gacacttccc aggtgaggcg ctgcagttcg
    33061 ccgtccgcgg ttaatgtcca ggagagttgt tgaccgattt tcaggttacg caattctttg
    33121 tcggcagcag ccagttgggt gatatcaccc atatcaatac catactgatt gagaatgctg
    33181 cttagcgtat cgccagtgga aacaacatat tcatgcacgc ccgcttcacc ggcgattttg
    33241 tcatccagtt cgtcctgggg aatggcttca tcttcttgtg cagcttgatc aatcggctca
    33301 ctggcttcag gtaagagcga acgaatttcg ttctgttcca gctcaatggt tttgacaatt
    33361 ggcgtggcat cacggtgata aacatagggc cgccagacag cgacggccag agtaagaacg
    33421 gtgagcgacc ccaacataac gcggtgtggt cgcggtaaat tattaaacgc cagggcgaca
    33481 gagcgggcta tctgttgcac gtaatcactt cctcattaat ctcctttcag gcagctcgca
    33541 tactggttgg ctaattgatt caggaattct gaatagcttg ttttacccag tttgatattc
    33601 gtccccaggg gatccaacgt tcccatacga acggatgtcc ctcgtgcgac gctctcaacg
    33661 accgctggcc tgaactgtgg ctcagcaaaa acgcaggttg ctttttgctc aaccaactgt
    33721 gttcttattt catgtaaacg ctgcgcgcca ggttgaatct cagggttaac ggtaaaatga
    33781 ccaagcggtg tcagtccgaa ctgtttttcg aagtagccgt aagcatcgtg aaaaacgaaa
    33841 taacctttcc ccttgagcgg cgcgagctcg ttaccaacct gcgtttcggt tgaggctaat
    33901 tgtgcctcaa aatccttcag gttggcgtca agtttggctc gactttgcgg cataagttcc
    33961 actaattttc catggattgc aaccgctgta gcccgcgcta tctctgggga aagccaaaga
    34021 tgcatgttga aatcgccgtg atggtgatct tcgtcacttt tttccgcgtg atcgtgatca
    34081 tcatcatcgt cgtgaatact tttcatcagc agcggtttca catcttctag ctgcgcaatc
    34141 gttacctgtt tcgcttctgg taatttactt accggttttt gcatgaacgc ttccatctcc
    34201 gggccaaccc aaacgactaa gtccgcgttc tgtaagcgtt ttacatccga tggacgcagt
    34261 gaataatcat gttctgaagc gccgtcaggc agtaaaacct gcgtttctgt taccccatca
    34321 gcaatggcag aagcgatgaa cccaacgggt ttaagcgaag cgacaacggc ggcatctgcg
    34381 gcctgtgttg caccgcccca gagagcggcg gataatgctg cgaaaagaag cgttttttta
    34441 tgtaacataa tgcgaccaat catcgtaatg aatatgagaa gtgtgatatt ataacatttc
    34501 atgactactg caagactaaa attaacatga caagtctggt ttccctggaa aatgtctcgg
    34561 tttcttttgg ccaacgccgc gtcctctctg atgtgtcgct ggaacttaaa cctggaaaaa
    34621 ttttgacttt acttgggcca aacggcgcag gtaagtcgac actggtacgg gtagtgctcg
    34681 gactggtaac acccgatgaa ggggttatca agcgcaacgg aaaactgcgc atcggctatg
    34741 taccgcagaa gctgtatctc gacaccacgt tgccactgac cgtaaaccgt tttttacgct
    34801 tacgccctgg cacacataaa gaagatattt tgcctgcact gaaacgtgtc caggccgggc
    34861 atctgattaa cgcaccgatg caaaagctct cgggtggcga aacgcagcgt gtactgttag
    34921 cgcgagcatt gttaaatcga ccgcaattat tagtgctgga tgaacccact cagggcgtgg
    34981 atgtgaatgg tcaggtggcg ttatatgacc ttattgacca actgcgtcgt gaactggatt
    35041 gtggcgtttt aatggtatct cacgatctgc atctggtaat ggcaaaaacc gatgaagtgc
    35101 tttgcctgaa tcaccacatt tgttgttccg gcacaccgga agttgtttcc ctgcatccgg
    35161 agtttatttc tatgtttggt cctcgtggtg ctgaacaact gggtatctat cgccatcatc
    35221 ataatcatcg tcacgattta cagggacgaa ttgttttgcg tcggggaaat gatcgctcat
    35281 gattgaatta ttatttcccg gttggttagc cgggatcatg ctcgcctgtg ccgcgggtcc
    35341 gctgggttcg tttgtagtct ggcgtcgtat gtcttatttc ggtgatacgc tggctcatgc
    35401 ctcattactt ggcgtcgcgt ttggtttgtt gctggacgtg aatccattct atgcggtgat
    35461 tgcagttacg ctgctgctgg cgggcggtct ggtatggctc gagaagcgtc cacagctggc
    35521 gatcgacacg ttattaggga ttatggcgca cagtgccctg tcgctgggcc tggtggtcgt
    35581 tagtctgatg tctaatattc gtgttgattt gatggcttac ctgttcggtg atttactggc
    35641 agtgacgcca gaagatctca tctctattgc gattggcgtg gtcatcgtgg tggctatttt
    35701 gttctggcaa tggcgcaatt tgctgtcgat gacgattagc ccggatctgg cgtttgttga
    35761 tggtgtgaaa ttacagcgcg tgaaattgtt gttgatgctg gtgacggcat tgacgattgg
    35821 tgtagcgatg aaattcgtcg gcgcgttgat tattacttca ctgctgatta ttcctgctgc
    35881 tactgcacgt cgctttgccc gcacgccgga acagatggct ggtgtcgctg ttttggtggg
    35941 gatggtggca gtgactggcg gtttaacctt ttccgcattt tacgatacac ctgcaggccc
    36001 gtcggtggtg ctatgcgcgg cactgttatt tattatcagt atgatgaaaa aacaggccag
    36061 ctaatctttc gctgaacata tttgtcggat gcggcgcgag cgccttatcc gaccttgtat
    36121 tatccctcca gtgcagagaa aatcggtcag ttttctctgc ctgcagtccg catgccttat
    36181 ccgacctacg gttcgctatc tctggtaggc ctgataagac gaac
//
LOCUS       DAPQZD010000033        35665 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPQZD010000033 DAPQZD010000000
VERSION     DAPQZD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.033"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..536
                     /locus_tag="RO454_001579"
     CDS             <1..536
                     /locus_tag="RO454_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDY0933426.1"
                     /translation="VYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEWEKLDAA
                     DAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDKQVTLIY
                     LQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQIPTGDLD
                     TAVKYGERVATITARLK"
     gene            complement(707..1699)
                     /gene="rpoS"
                     /locus_tag="RO454_001580"
     CDS             complement(707..1699)
                     /gene="rpoS"
                     /locus_tag="RO454_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDY0933427.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1762..2901)
                     /gene="nlpD"
                     /locus_tag="RO454_001581"
     CDS             complement(1762..2901)
                     /gene="nlpD"
                     /locus_tag="RO454_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDY0933428.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3041..3667)
                     /gene="pcm"
                     /locus_tag="RO454_001582"
     CDS             complement(3041..3667)
                     /gene="pcm"
                     /locus_tag="RO454_001582"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDY0933429.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3661..4422)
                     /gene="surE"
                     /locus_tag="RO454_001583"
     CDS             complement(3661..4422)
                     /gene="surE"
                     /locus_tag="RO454_001583"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDY0933430.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4403..5452)
                     /gene="truD"
                     /locus_tag="RO454_001584"
     CDS             complement(4403..5452)
                     /gene="truD"
                     /locus_tag="RO454_001584"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDY0933431.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5449..5928)
                     /gene="ispF"
                     /locus_tag="RO454_001585"
     CDS             complement(5449..5928)
                     /gene="ispF"
                     /locus_tag="RO454_001585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDY0933432.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5928..6638)
                     /gene="ispD"
                     /locus_tag="RO454_001586"
     CDS             complement(5928..6638)
                     /gene="ispD"
                     /locus_tag="RO454_001586"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDY0933433.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6657..6968)
                     /gene="ftsB"
                     /locus_tag="RO454_001587"
     CDS             complement(6657..6968)
                     /gene="ftsB"
                     /locus_tag="RO454_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDY0933434.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7161..7484)
                     /locus_tag="RO454_001588"
     CDS             complement(7161..7484)
                     /locus_tag="RO454_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDY0933435.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7533..8138)
                     /gene="cysC"
                     /locus_tag="RO454_001589"
     CDS             complement(7533..8138)
                     /gene="cysC"
                     /locus_tag="RO454_001589"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDY0933436.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8138..9565)
                     /gene="cysN"
                     /locus_tag="RO454_001590"
     CDS             complement(8138..9565)
                     /gene="cysN"
                     /locus_tag="RO454_001590"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDY0933437.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9567..10475)
                     /gene="cysD"
                     /locus_tag="RO454_001591"
     CDS             complement(9567..10475)
                     /gene="cysD"
                     /locus_tag="RO454_001591"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDY0933438.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10727..11764
                     /gene="iap"
                     /locus_tag="RO454_001592"
     CDS             10727..11764
                     /gene="iap"
                     /locus_tag="RO454_001592"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDY0933439.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11890..12042)
                     /locus_tag="RO454_001593"
     CDS             complement(11890..12042)
                     /locus_tag="RO454_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDY0933440.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12308..13042)
                     /gene="cysH"
                     /locus_tag="RO454_001594"
     CDS             complement(12308..13042)
                     /gene="cysH"
                     /locus_tag="RO454_001594"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDY0933441.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13116..14828)
                     /gene="cysI"
                     /locus_tag="RO454_001595"
     CDS             complement(13116..14828)
                     /gene="cysI"
                     /locus_tag="RO454_001595"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDY0933442.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14828..16627)
                     /gene="cysJ"
                     /locus_tag="RO454_001596"
     CDS             complement(14828..16627)
                     /gene="cysJ"
                     /locus_tag="RO454_001596"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDY0933443.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            16946..17308
                     /gene="queD"
                     /locus_tag="RO454_001597"
     CDS             16946..17308
                     /gene="queD"
                     /locus_tag="RO454_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDY0933444.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            17386..18657
                     /locus_tag="RO454_001598"
     CDS             17386..18657
                     /locus_tag="RO454_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDY0933445.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18648..18908
                     /locus_tag="RO454_001599"
     CDS             18648..18908
                     /locus_tag="RO454_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDY0933446.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18925..19500
                     /locus_tag="RO454_001600"
     CDS             18925..19500
                     /locus_tag="RO454_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDY0933447.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19648..20508)
                     /locus_tag="RO454_001601"
     CDS             complement(19648..20508)
                     /locus_tag="RO454_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDY0933448.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20505..21284)
                     /locus_tag="RO454_001602"
     CDS             complement(20505..21284)
                     /locus_tag="RO454_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDY0933449.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(21262..22671)
                     /locus_tag="RO454_001603"
     CDS             complement(21262..22671)
                     /locus_tag="RO454_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933450.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22693..24147)
                     /locus_tag="RO454_001604"
     CDS             complement(22693..24147)
                     /locus_tag="RO454_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDY0933451.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKKVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24217..25002)
                     /locus_tag="RO454_001605"
     CDS             complement(24217..25002)
                     /locus_tag="RO454_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0933452.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            25321..26598
                     /locus_tag="RO454_001606"
     CDS             25321..26598
                     /locus_tag="RO454_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933453.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            26625..28103
                     /locus_tag="RO454_001607"
     CDS             26625..28103
                     /locus_tag="RO454_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0933454.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            28268..29188
                     /locus_tag="RO454_001608"
     CDS             28268..29188
                     /locus_tag="RO454_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDY0933455.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            29356..30873
                     /locus_tag="RO454_001609"
     CDS             29356..30873
                     /locus_tag="RO454_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDY0933456.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30934..32304
                     /locus_tag="RO454_001610"
     CDS             30934..32304
                     /locus_tag="RO454_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDY0933457.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            32304..33713
                     /locus_tag="RO454_001611"
     CDS             32304..33713
                     /locus_tag="RO454_001611"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDY0933458.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            33739..34746
                     /locus_tag="RO454_001612"
     CDS             33739..34746
                     /locus_tag="RO454_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDY0933459.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34831..35502)
                     /gene="queE"
                     /locus_tag="RO454_001613"
     CDS             complement(34831..35502)
                     /gene="queE"
                     /locus_tag="RO454_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDY0933460.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         8643 a         9253 c         8860 g         8909 t
ORIGIN      
        1 tggtttatta ttcaggatat ggtcacacta aactggttgc tgaaaaagtg gcacaaggcg
       61 cccatgcaga tttaatttcg attgataacg aaggaaatat cactgagcag gagtgggaaa
      121 aactggacgc agccgatgcc attatcttcg gtgctcccac ctatatgggc ggtgcgccat
      181 ggcaattcaa gaaatttgct gatgccagtt ccaaagcgtg gtttacacgt aaatggcagg
      241 ataaagtctt cgggggtttc accaacagcg ccagcctgaa tggcgataaa caagttacgc
      301 tgatctatct gcaaacgctg gcttcacagc atggcggtct gtgggtgagt ttaggtcagg
      361 ctcctgccaa tgtgcttgcg tcaaccaggg aagatgtaaa caatctgggc ggttcggtcg
      421 gtttgctggt gcaatctcca gccgatgcgg gtgcagagca aatccctacg ggcgatctgg
      481 atacagccgt gaaatatggt gaacgcgtgg cgacaattac tgcgcgctta aaatgatgat
      541 tgccgaatgt gatgctgacg cgtctatccg gtcttctaca tctaaaaagt gccgtcccct
      601 ggtcagtcag ataaagtttt aacgctgtat ccgataacag ttcaacatgt accaaaagaa
      661 aaaaagccag cctcgcttga gactggcctt tctgacagat gcttacttat tcgcggaaca
      721 gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg caaacggcgc aggccttcaa
      781 cctgaatctg gcgaacacgt tcacgggtga ggccaatttc acgacctaca tcttccagtg
      841 ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag cacttcacgc tgtttggcgt
      901 tcagctcgaa cagccatttg acgatgctct gcttcatatc gtcatcttgc gtggtatctt
      961 ccggaccatt ctctttttca tcggccagga tgtccagcaa tgctttttcg gaatcaccac
     1021 ccagcggggt gtctaccgag gtaatgcgct cgttaagacg aagcatacgg ctgacgtcat
     1081 caactggctt atccagttgc tctgcgatct cttccgcact tggttcgtgg tccagcttat
     1141 gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc ctttacgatg tgaatcggca
     1201 aacgaatagt acgggtttgg ttcataatcg cccgttcgat cgtctggcga atccaccagg
     1261 ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa cttctctacc gcgcggatca
     1321 gccccaggtt gccctcttcg atcaggtcca gcaacgccag accacgattg ccataacggc
     1381 gggcaatttt taccaccaga cgcaagttac tctcgatcat ccggcggcga gaggcgacat
     1441 ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc ggccgttaac agtggtgaat
     1501 aaccaatctc accaaggtaa agctgagtcg cgtccagcac acgctgtgtg gctccctgcg
     1561 ataacagttc ctcttcggcc aaatcgttat cactgggttc ctcttctact aaggcctttt
     1621 cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc ttcatttaaa tcatgaactt
     1681 tcagcgtatt ctgactcata aggtggctcc tacccgtgat cccttgacgg aacattcaag
     1741 caaaagcctg gttccgccga tttatcgctg cggcaaataa cgcagcgggt ttacggattt
     1801 ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg gttccggtgc tacccatggt
     1861 tgctattttt tgccccgcct tcacttcttg ttgttcccgg accagcattg tgtcgttatg
     1921 ggcgtaggca ctcaggtaat catcattatg tttgatgata atcagattac cgtagccgcg
     1981 cagcgcgtta ccggcataaa caacgcggcc atctgcggtc gcgataattg cctgtccttt
     2041 gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa gcgccaaagg tttcgatcac
     2101 tttgccctca gtcggccagc gccaggtgga gataggcgta ctggttgatg tactgctgac
     2161 agtcggctcg gttgtgcttg ctgttggtac cgttacaggc actgtgaccg tggtcgcagt
     2221 tggcttgttg ttcggcaaca ttttgttagc actctgttca cccgaagact cagaatacgt
     2281 aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt gcaggcttga tcacaactcc
     2341 ttgctctgct gcgtcggcct gggtaatggc atttccgcct gtgattggcg taccggaagc
     2401 attacccacc tgtaaggtct gaccgacgtt cagcgcgtat ggtgcctgaa tattgttgcg
     2461 ctgagcaagg tcacggaaat cgttgccagt aatccaggcg atatagaaaa gcgtgtcgcc
     2521 ttttttcacg gtgtatgtac tgccgctata actgcctttc ggaatgttcc catactgacg
     2581 gttgtagacg attcgtccgt tttccatctg taccggctgc tgagctactg gctgcacagg
     2641 ctggatttgc ggttgttgag tagcctgaat ttgtggctgc tgtaccggct gaatttgcgg
     2701 ttgctgcgct gtagacgtcg tccccatttt cggcggcggc gtaatcaaca taccagaatt
     2761 ggtatttgcg ggcgcattgc cattaacgga gctgaccggt gccggtggat ttgaagtgtc
     2821 agaacagcct gccagccata gcgaaaccag tgacaatgcc gcaatgcggc gaacggtgaa
     2881 ttttgggctt cccgcgctca tttatccccc aggaaaaatt ggttaataac cagtgacata
     2941 attaccgtgc aaggcaccat actgaacact ggaaaagatg ttcacgatac gctgacttgc
     3001 ggcaaaataa ccaggaaaaa tccaggtatt tcctcacgtt ttaagccagc tcacccttca
     3061 ctaaagggac aaagcgcacg gcctccacgg tatcgataat aaattcgcct ccccgacgac
     3121 gcacccgttt caaatactgg tgctcctccc ctacgggtaa gacgagaatc ccgccttcgt
     3181 ccagctgcgt cattagcgca gttggaattt caggcggtgc cgccgtaaca atgatagcgt
     3241 caaacggcgc gcgtgcctgc caaccttgcc atccatcgcc atgacgggtt gaaacattat
     3301 gtaaatcaag atttttcagg cggcgacgcg cctgccactg caagccttta atccgttcaa
     3361 ccgagcaaac atgctggaca agatgcgcca ggattgcagt ttgatatccc gaaccagtgc
     3421 caatttccag cacccgcgac tgcggcgtta gctcgagtaa ttcggtcatt cgcgccacca
     3481 tatacggctg cgaaattgtc tgcccctgac ctatcggcaa ggcgatattg tcccaggctt
     3541 tttgttcaaa cgcttcatca acgaattttt cacgcggcac ggcggcaagt gcattcagca
     3601 cctgctcatc ctgaatacct tgcgcacgta attgatccag aagtgcttgt acgcgtctgc
     3661 ttaccattgc gtgccaactc ccacgctgtt taaccagtct gaaaccacat cttgcgcgct
     3721 atgcgcagtt aaatccacat gcagcggcgt gatggaaaca tagccctcat ctaccgcagc
     3781 aaaatcggtc cccggaccgg catcacattt accgcccggc gggccaatcc agtacagcgt
     3841 attgccgcgc ggatcttgct gcgggatcac ctgatctgcc ggatgtcgtg taccgcagcg
     3901 cgtcacgcga atacctttga tttgatccaa tggtaaatcc ggaacgttaa tattaagaat
     3961 acgcccggtg cgcagcggct ctttacacaa tgcgcgcaaa attgagcagg ttaccgccgc
     4021 ggcagtgtcg taatgtttat gcccgtcaag cgagacggca agcgccggaa aacctaaatg
     4081 acggccttcc atcgcggccg ctaccgtacc ggaataaata acatcatccc ccagattcgg
     4141 cccggcgtta attccggaca caacaatgtc cgggcgcggg cgcatcagag catttacgcc
     4201 aagatagacg caatcggtcg gggttcccat ttgcacagca atatcaccat tttcaaaggt
     4261 aaacgtgcgc agggaagatt ccagtgtcag agaattagaa gcgccgctgc ggttacgatc
     4321 gggggcgacc acctgaacgt cagcaaactc acgcaaggct ttcgccagcg tttgtatacc
     4381 gggtgcatgt accccgtcat cattactcag caatatgcgc ataatcacct gttgtgttga
     4441 taagttccct gacaacgctg gttgcaaaac ttcccgccgg aagccagaaa cggatttcta
     4501 cggtgacgtc atcccaccag ttccagctta attgttgcgg atacagcagc atcgctctgc
     4561 gcgcggcttc aactttttca cgcaccagta aagtttgtaa ttcagtttct tcggcgacag
     4621 ctgcttgttc gaatgccagc gcttcacgct gagttcccca ttcgccactg cccagcaatg
     4681 cggcggttat cattagctct ttatcgttga cgcgacgctg taattccgcc agttcttcgg
     4741 tggttgcaac aaaccagcta ccacgtccgg ctaattgtag cgcatcgccg tcaacaactt
     4801 gattaacgtc tgcttttttg aggcgctcag caacaatctg attaaacaac gcactgcggg
     4861 ctgccgacaa ccaaaaactc cgtttattgc gatcgcgcac cggagtattg gtttgcgccc
     4921 agcgcagcgc gccctgcaag ttgctaccgc caatcccaaa acgttgggca ccgaagtagt
     4981 tcggtacacc ttttacgcaa atatcgatca gacgttgttc aacgtcatcg cgattgctca
     5041 cttcgcgcag aaccagggta aaggcgttac ctttcagcgc gcctaaacgc agcttgcgct
     5101 tgtgccgcgc atactccagc acctggcagc cttccagttg aaaggcgctc agatcgggca
     5161 tctctttgcc cggcacgcga gcgcataacc actgttccgt aacagcatgt ttatcttttt
     5221 gcccagcgaa gctgacttca cgggcatgaa ttttcaggaa tttcgccagt gcatccgcca
     5281 caaaacgggt attgcagcca tttttgagga ttctaaccag aatatgctca ccttcaccat
     5341 caggctcaaa gcccaaatct tccaccacca caaagtcttc cggattggct ttcagcagcc
     5401 cggtgccttg cggtttaccg tggaggtaag tgagattatc aaactcaatc attttgttgc
     5461 cttaatgagt agcgccaccg cttcacaggc aatcccttcc ccacgtccgg taaatcccag
     5521 tttttccgta gtagtggctt tcacgttaac atcatccata tggcagccga gatcttcggc
     5581 aataaacacg cgcatttgtg gaatgtgcgg caacatcttc ggtgcctgag cgatgatagt
     5641 gacatcgacg ttgccgaggg cataaccttt cgcctgaata cgacgccagg cttcgcgcaa
     5701 cagctcgcgg ctgtcggcac ctttaaatgc cggatcggta tccgggaaca gcttgcctat
     5761 atctcccagc gccgccgcgc caagcaatgc atcggtcaac gcatgcagcg ccacgtcgcc
     5821 atcagaatgc gccagcagcc ctttttcgta aggaatgcgt acgccaccaa tgataattgg
     5881 gccttcaccg ccaaaggcgt gtacgtcaaa accgtgtcca attcgcatta tgtattctcc
     5941 tgatggatgg ttcgggtgag gtaaaattcg gccagcgcca aatcctccgg gcgcgttacc
     6001 ttgatgttat ccgcgcggcc ttcaacaagt tgcggatgaa aaccgcaata ttccagcgcc
     6061 gaggcctcat cggtaatggt agcgccttca tttagagcgc gcgtcaggca gtcatgtaac
     6121 agctcacgag ggaaaaattg cggcgtcagc gcgtgccata agccgttgcg atcaacggta
     6181 tgagcaatgg catttttgcc cggttcggca cgtttcatcg tatcgcgcac cggcgcagcg
     6241 aggatccctc ccgtgcggct ggtttcgctc aacgccaaca atcgcgcgag gtcatcctga
     6301 tgcagacaag gacgagcggc gtcatgcacc aatacccact gcgcgtcgcc agcggctttc
     6361 agacctgcca gcacggaatc ggcacgctct tcaccaccat caacaaccgt gattcgtgga
     6421 tgattcgcca gaggaagttg tgcaaagcgg ctatcgccag gacttatggc aatgacgaca
     6481 cgtttcaccc ggggatgcgc cagcagcgca tgcaccgagt gttcaagaat ggtttgatta
     6541 ccgattgaga gatattgctt aggacattcc gtttgcattc ggcggccaaa tccggccgcc
     6601 ggaaccacgg cgcaaacatc caaatgagtg gttgccatgt taattcccgg gctgatttat
     6661 cgattgtttt gccccgcaga ctgtgcgcgc tttgacgcgt caggcaccag acgataaaac
     6721 gtttcgcccg gcctggtcat gctgagttca ttacgcgcac gctcttcgag cgcctcctgg
     6781 ccgccattga gatcgtcaat ttcggcaaaa agctgatcgt ttcgcgcttt aagtttcgcg
     6841 tttgtagctt gctgtgccgc cacatcatta ttgacgcgag tatagtcatg tataccgttc
     6901 ttaccgaacc acagcgaata ctgtagccag accagaatag ccagcaacag cagcgttagt
     6961 ttacccatcc tgccccctga aaaacggcat cttcatccca tgcatccgaa gacgactcta
     7021 catcctctgt tggggatacc gcgacaacgc gggcaaatgt accacatttg tccattgtta
     7081 cgtataccca gggcgtgcag aaaataatct cattattagt tacggattga attatgaaca
     7141 gaatagacgg gaaagaacaa ttaacccaat agccacataa acagtgcacc aaacataatg
     7201 ccaaccgtcg ccagagtgaa aacaatactg tagcgtagct ttccgtccat caatgaatgc
     7261 agtgcaatcc ccaccactac agcgacgggc atcagcgcca gaaagaaagg ccaggtgtag
     7321 ataaaaaaga gcagcgtgtt agagccataa atcaacatcg gaattgccaa cgcgcacagc
     7381 cacgataaaa aaccaaccac agcgcctggc agcgaccatg tggtgtcttc aacctcagta
     7441 aggctgtcgt tatttgttaa tgtaatgttg tggctgttac gcatatctga tcctgttact
     7501 ttgacgaacc gggcatgaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc
     7561 aacagatcta ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt
     7621 tctgccgatt caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg
     7681 gcacgcgctt tcttatataa accttttgga tcgcgggctt cgcaaatcgc cagcggcgta
     7741 tcgacaaaca cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg
     7801 gcgcggtgtg gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc
     7861 gccacttcac cgacgcgacg gatattctct ttacggtcgg catcgctaaa accgagatcg
     7921 ctgcataatc cgtggcgaac gttgtcgcca tccagcagat acgtactgac gccaagttta
     7981 tgtaacgcct cctccagcgc ccccgcgacg gttgatttac cggaaccaga gaggccggta
     8041 aaccacagca caacaccacg atgaccgtgg tgctgttcgc gttgttgcag agttactggg
     8101 tggctatgcc agacgacgtt ttcgtcatgc agagccatta tttatctccc agtaaatcgc
     8161 gcgcacccca atgcgggaaa tggcggcgaa ccagggcatt caattccagt tcgaatgcac
     8221 tgaattcaga tggcgcagca gttgcctggc taactggttc gtgcaccata ccggcaccta
     8281 cggtcacatt actcaggcga tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat
     8341 aacgatctaa caccagtggc tcgtcaaagg tgagatctac caggccgatg ccgttcagcg
     8401 gcaggttttc aacttcgcgt tgggtaaggt tattgatatc aacctgataa cgaatgccat
     8461 caacacgagc acgcgtcttc ttaccggcaa ttttgatgtc gtaactctgg cccggggaaa
     8521 gcggctgttc cgccatccat accacatcca ccgacgcgct ctgcacagcc ggtaacactt
     8581 cgtctgccgc cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg
     8641 tgatcgcttc tccggcaaag gcttcttcgc gatcgccatc aaaggtcacg atccgcgcga
     8701 cgtttgattc cacaccagag ggcagcactt tgacacgttg cccgacttcc acacgaccgg
     8761 atgccagcgt tccggcgtag ccacgaaaat cgagattcgg acggttaacg tactgcaccg
     8821 ggaagcgcat tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga
     8881 gcagtgtcgg accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt
     8941 ccagtgcgga gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaagg
     9001 tcagataatc ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt
     9061 tgttgatcgc cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac
     9121 gggtttgatc gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg
     9181 atgcgccagt cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa
     9241 atttacgctt ctcggtagag aaatagcggt aagccacgtc aatggtgatg ccctgttcgc
     9301 gctcagcttg caggccgtcc accagcagtg ccagatccag cttttcgccc tgggtgccgt
     9361 gacgcttact gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt
     9421 gtagcaggcg accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac
     9481 gcagcaggct tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga
     9541 tttgttgtgc aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata
     9601 gaccccgcct ggtcgcggtc aattacgcgg ccctgacgtt cactggtggt ggaaaccagc
     9661 atctcttcga tgatctccgg cagtgtttgt gcgtttgact ccaccgcgcc ggtcagcggc
     9721 cagcagccca gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg
     9781 tcgatacggt tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc
     9841 gcagcgagat acagtggaac aatgtcaata ttttccagcc agatgtattg ccagatatcc
     9901 tgctcggtcc agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg
     9961 ttgtagttgt gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca
    10021 cggaaagagt agattcgctc tttagcgcgg gatttctctt cgtcgcggcg cgcaccaccg
    10081 aaggcggcat caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata
    10141 tcggtatgtt tcgcgctgcc atgaacgaat ggattaatcc ccatcgctac gccttccggg
    10201 tttttatgca ccagcagctc gcagccgtag gctttagccg tacgatcgcg gaactcatac
    10261 atctcgcgga atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga
    10321 taaaacgcct tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc
    10381 atcaccggat ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc
    10441 agttgccgca ggtgggtaag tcgtttttga tccataaccg ttcctttgca ataccgctat
    10501 tttcttgcca tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat
    10561 tagaatgagt agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt
    10621 aaggcaccgg atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt
    10681 catactatgg cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt
    10741 gcgccaccgt accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc
    10801 gtcacctaaa cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc
    10861 gggacgcatg accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt
    10921 tcagcaaatg ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc
    10981 ccgcgataat cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga
    11041 aggcaaaacg ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag
    11101 cgatgctgac gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc
    11161 cgcaggttta ggtgtcatgc tggaactggc cgagcgcctg aaaaatacgc ctaccgagta
    11221 tggtattcga tttgtggcga ccagcggcga agaggaagga aaattaggcg ctgagaattt
    11281 actcaagcgg atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa
    11341 cttaattgtt ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag
    11401 gaaattaacg cgcgaccggg cgctggcaat tgcccgcagc catggaattg ccgccaccac
    11461 aaatccaggt ttgaataaaa actatccgaa aggcaccggg tgttgtaatg acgcagaaat
    11521 attcgacaaa gccggtattg ctgtactttc ggtggaagcg actaactgga atcttggcaa
    11581 taaggatggt tatcagcaac gagcaaaaac agccgcattc cctgcgggaa atagctggca
    11641 tgacgtaaga ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg
    11701 tcgctgccgt gatgttatgc ggataatgct accgttggtg aaggagctgg cgaaggcatc
    11761 ttaatgggtt tgaaatgatt actcccttgc ctgcaggaaa tcaatgccac cagaaacaga
    11821 gtataaaaaa ggggtgcagg aggccaaacc atcacaattc agtacgcggg gttttacccc
    11881 gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca taccacgttc
    11941 tctgatactc aactcacaca acgagtcgcc taccatcaac gtgaatccca gtactgtaca
    12001 acacagtacg atgattgcca caagggcata ttttgtcagc atgtcttgcc tccctgcaaa
    12061 gaagagacta acatccgaat tgctacgttc gaacgcgagc ctcaggttga tagaaatatc
    12121 gcctggggct tttgtccatc tggaaccttg cgaatgctta acgccagaca gcctcaagca
    12181 cccaatgcca ttctatacct gttaattttc cctgcgatgt tcacctttaa atgccggaat
    12241 gctgctcgca aaaaataaaa acgcctccat catttctgac agaggcgttt aatttatccg
    12301 gcaatattta cccttcgtgt aatccacatt ccctttttaa gccaaagaaa cgcgtttctt
    12361 cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc gataaatatc
    12421 cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga taaatagttc
    12481 ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg
    12541 gtaaattggc gcggctgccg gattgttcac ggcgcaggcc agcaaaccag gtttgcgcat
    12601 tcagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa
    12661 tgccttcaac gccctgctcc cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt
    12721 cggtagcacg atacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc
    12781 ggtaggtttc cgggaacaga tagccggtat cggtgaggat cactggaata tccgggcgaa
    12841 tctgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat
    12901 attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc agtttttcca
    12961 gttgggcgtt agtttccgcc agcgccagaa tccggtctac ctttggcagt tcgttcaggg
    13021 cgtttagatc gagtttggac ataagttcct cactgtttgc cttgcctgat gcaacgctca
    13081 cacgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
    13141 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
    13201 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
    13261 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
    13321 aggttgtagc gacccggcgc tttccccacc agacccactt ccgccagcat cgcgcgacca
    13381 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
    13441 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc ttccgccatc
    13501 gccagcgggc aagtcgggaa tgacacgcag gccattgagt tttcacgctg cggcgtgacg
    13561 gcattcatta acccgctttc tttggcgatc ttctcgatct tcgctttctc gctttccggc
    13621 acgccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt gtggatcttc
    13681 gcgatctcca gcaagccggt tttcagcggg cgccccggat aatcaagaat tcgaccattt
    13741 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatcg
    13801 ccgcgtccgg taaactcata cgggcgaatc ggctcgaatt tgatccccgc gcgacgttca
    13861 acttccgctt taaacgtctc aacccctacg cgctccagcg tgtatttggt tttggcattt
    13921 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc cgccaccgcc
    13981 agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta ggttttcttg
    14041 ttgccgtgtt cgatggaaag cccaccgccc accagcaggt taaagcccac cagcttgccg
    14101 ttttcggcga tcgccacgaa gttcatgtcg ttggcgtgca gatcgatatc gttctgcggc
    14161 gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag gatcggctct
    14221 tcatcagtag tggcgacttt ttcctgatcg agccagatct ccgcatacgc gcgggtacga
    14281 ggcagcagat gttcagaaat tttcttcgcc cattcatatg cttccgcatg cagctgcgac
    14341 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttggcggt cgccagcgca
    14401 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
    14461 tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
    14521 gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg cagacgacag
    14581 cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc gcgaatgtcg
    14641 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc gcccttaaag
    14701 ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
    14761 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
    14821 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
    14881 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
    14941 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
    15001 tccagcgcca cagctctgcg ccctgttcgc gcagtttgtc ttgtacgtaa attttttctt
    15061 tttgatcgcg cgaccaggcg agatcgatgc gcgtcagcac gccttctttg acgtaacgct
    15121 gccattccac ctggtagagg aaatcttccg taaagtgtgg gttgccaaag aatagccagt
    15181 ttttacccgg cgcttcgtcg gcggcacgct gctgcataaa ggcacggaac ggcgcgatgc
    15241 cggtgccagg gccaatcatg atcaccgggg tttccggatt ggttggcagg cggaagttgt
    15301 cgttatgttc gataaatacg cggacttcac cctcttcttc cacacgatcc gcgaggaagc
    15361 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtaa
    15421 cgtgcacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg cgcggggtca
    15481 gtgggcgcag cagattaatc agcgcttcgg catccagttg cgccggagag aaacgcacca
    15541 tgtcgacaat cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
    15601 gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg gcggtgttga
    15661 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
    15721 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccggat
    15781 cgttttgata ccagacgccc agcgcgtcac ccggctggta acgcaggtca gagtcaccta
    15841 agtcaatttc aatgtgacga acgtcttttt cagagttacg cccggtaatt ttctggttaa
    15901 cagagaggct cgccaccagc ggcgcgtctt tgctatacgg gctggtgtgg atttcattta
    15961 ccgtgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
    16021 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg gcatcgacac
    16081 ggtcgagcag gcgttcaccg cccagttccg ccagcttact gtcaaaatct ttcccggact
    16141 ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca
    16201 gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
    16261 ccccttcccc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg atttgtttga
    16321 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgctaat aaatcatcgc
    16381 gtaatgcttc agcaacccga cgcgcattgc cagtttgcga ggcggagata atagttatac
    16441 ccggcatttc tgcggcgggc gctggcgtcg ctgcaagcgc agcaggctgc tggttgagca
    16501 cgccccagaa atagccagaa acccaggcaa gctgggtagg agttaaatcg gtcgtggccg
    16561 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
    16621 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
    16681 aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
    16741 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
    16801 taaccaaccc aatgaaaaac aaatgaatat tgcccatcaa taagataaat cagcgatttt
    16861 gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt
    16921 tttatcgtca tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
    16981 agccgctcac cgcttgccgc acgtcccaca agggcataaa tgtggtcgcc tgcacgggca
    17041 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
    17101 cgatttcgct gaactgaaag cggcgtttaa accaacttat gagcgcctcg atcaccatta
    17161 tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa aatggatttg
    17221 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
    17281 cgcaggttgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag
    17341 taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga agacgactgc
    17401 gacattatta ttatcggtgc cggaattgct ggcaccgctt gcgcgttacg ctgcgcgcga
    17461 gcgggtttat ccgttttgtt actggaacga gcggagattc ccggcagcaa aaatctttcc
    17521 ggcgggcgtt tatataccca tgcgctcgcg gaactcctcc cgcaatttca cctgaccgcg
    17581 ccccttgaac gacgcatcac tcacgaaagt ctttctctgt taacgccaga tggcgcaacg
    17641 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acgattcgat
    17701 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg tgcgacggta
    17761 gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga cgatattctt
    17821 cacgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggcgga acgccacggg
    17881 ttagtgactc gtcctgctgg cgaagcgatg gcattgggaa tcaaagaagt gctgacgctg
    17941 gaaccatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgttg
    18001 ttcagcggcg ggatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa
    18061 acgctctcgt tagggattgt ttgcccgctc tcctccctta cgcaaagtag tgttccggca
    18121 agcgagctgc tgactcgctt taaaacgcat ccggcagtgc gcccgcttat caaaaacacg
    18181 gaattactgg agtatggtgc gcatctggtg ccagaaggtg gcctgcgcag tatgccggtg
    18241 caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc
    18301 ggaatttccg tgcgcggcat ggatatggcg ctaactggcg cgcaggcggc ggcacaaacg
    18361 ctgataagcg cctgccaaca ccgcgagccg caaaatctgt ttccgcttta tcatcacaac
    18421 gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc ggcgcttttg
    18481 caacgtcctg gctggtatcg ggtgtggcct gggttaatgc aggatatttc ccgtgattta
    18541 tgggatcagg ggaatacacc tgttccaccg ctgcgccagt tgctctggcg tcatttacgt
    18601 cgtcacggcc tgtggcatct gacgggcgat gttatcagga gtctgcgatg tctgtagccc
    18661 gtaatctctg gcgcgctgct gatgcgccgc acattgttcc gactgactcc gttgagcgcc
    18721 agacggcaca acggttgatt aacgcctgtc cagcaggtct tttttcgctc acacctgaag
    18781 gtggcttacg tgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg
    18841 acgaatcaac actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgct
    18901 ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca gaatccggtg
    18961 attgctgccg ttaaagataa tgccagcctg caactggcaa tcgactctga atgccagttt
    19021 atttccgtgt tgtacggcaa tatctgcacc attagtaata tcgttaaaaa gattaagaac
    19081 gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtg
    19141 gtgattcagt ttttgaaact ggtaaccgag gcggacggaa ttatcagtac caaagcctca
    19201 atgctgaaag cagcaagagc ggaaggtttt ttctgtattc atcgactgtt tattgttgat
    19261 tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag
    19321 atcctgccag gctgcatgcc taaagtgctg ggctgggtga cggagaaaat ccgccaaccg
    19381 ctgattgctg gcgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt
    19441 gttgtggcgc tttccaccac gaatactggg gtctggacgt tagcgaaaaa attgctttga
    19501 cgggataatc gtaacaagtt gaatttggtg tgatttgtag gctgcacgcc acatccgaca
    19561 ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc cagcccagaa
    19621 ccatcggcag gatcatccgg cagcacgtta ttggcaatct gcgtgaatgt ttgtcaccag
    19681 tgcctccagc accactttcc agtcatcaac tacgccaaca tctgcctgtg aaaataccgc
    19741 tgcactggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa
    19801 cgctgctgcg ccggaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga
    19861 aataccaatc actttttcgg catcaacgcc accgttcatc actcgtgccc gactgtagcc
    19921 cacttcggct cccatctttt ctgccagcat agcgatttcc tggttatctg cctctccgcc
    19981 ctgccccacc accagcaccc gtctggcttc agctaatggg tcgcggataa catttttcag
    20041 attttctatg ctaaccagcc agtcgggaag cacacctggg acaatgttca gttgctgcat
    20101 cccagagggc aacgtggcgt ttttatctgc tcccgcctga cgtgccagcg aaaggcatag
    20161 cgggcgtttg tctgtttgca gcgtcgccgt caacgcattt ccccagtggg atttgcgcac
    20221 gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca
    20281 ggccagccgg gtcgacagtt catcgccaaa cgcgcccgga ggaaaaagga caacgtccgc
    20341 aggcgttcgt tgccactgtt ccaccagtgc atccagcacc tgttccgcaa ccatcggctg
    20401 tggttctatt tgccaatgtg ccagggtaca accgctgaaa tcctgcgccg ccagccagct
    20461 cgctattgcg gcatggtcct gatttattgt cacaattacg atattcatgg ctgcatcctt
    20521 tggcgtaaat agtcctgcca tagcttttgc gctttttctg ctaccgtttg cccaccaatc
    20581 agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcaat
    20641 tcgacagcaa cagttttacg gataattgct gctttccccg cagccattcg ctggcgcatt
    20701 cccggaactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga
    20761 acccggcaac agcgcacccc atgttcagta cgctgttcga gggtgataaa cggtgcgtcg
    20821 agagtaaagc gctccacctg ggtgaagcag ggccagacca gcatttccgc cagcaaaaat
    20881 ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga
    20941 ttctgacgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct
    21001 gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag
    21061 tgcaacgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt
    21121 ctctgcgcca acagcagcgc agctgcggcc tgttcatcag caccgagtaa acttcgcaga
    21181 agcgaaacat ccggtccgct attaccctga gccgccgcct gccactcttt ttccgccagc
    21241 attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt
    21301 tcccgccgcc accagcggga gtgctttggt ttctggtgcc catagccagg tcacaacaaa
    21361 accaaccaac aacactgccg ccaggagtaa taaggtggct tgcattcccc actgcgccag
    21421 cacccacggc agcaggccag tacttaccgc cgcccccaac cgactcatgg cggtggcaaa
    21481 accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag
    21541 attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact
    21601 gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa aacttcccag
    21661 caaaaacttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg cgcccacaat
    21721 taacaacgca ttaagcatca gactggcagt cagcgcatct tccagtccaa tagtctgggc
    21781 gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga caaagaagac
    21841 gctgttaaac gctgtgcgcc gccagtagcg cgaagagaac aaggttttga tgtgtttatg
    21901 ggtcgccgtt gccacttcat cgcccagtaa aacatgcggg ccaaaatagc gatgaacgat
    21961 agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggcgatt ccggcgtgcc
    22021 ccaacgtagc agcgtaatca gcaaggctgg taacgccgct gatgccagca gccagcgcca
    22081 ggcctccggg ctttcggaaa taaagtgatg tccggcaata cttgccagca catagcctac
    22141 ggtccacacc acactgaatg cgcccagcaa aataccgcga tggcggcgcg gggaaaattc
    22201 agccagcaag gtgtgaccga ctgagtaatc gcctcccaga ccaatgccga tcaaaatgcg
    22261 taatccaata agatgctctg gcgtggtggc aaaaaattgc aagaacgaag cgagcgtaat
    22321 cagcataaaa ctgaaggtga agattttttg ccgcccgata tggtcggaga tccaccccaa
    22381 aaccaggctg ccaagaaaca aaccaagtaa cgccgagccg ccgatcattc ccgccataaa
    22441 cggcgtcagt tgcatggcgg gcgtaagctg aataatggca taaccaatga cgccgagaac
    22501 ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca attcgacagt gaaaacggtt
    22561 aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt cattgtccgg
    22621 taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgcaccgtca tcgcggaacg
    22681 ctgcctgaca cattattttt caatcggata gatagtaccc gtgttcataa tgccattagg
    22741 atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct
    22801 ccagtgaacg cgatgtttac cgataccgtg gtgatgcacc atcgaaccgc cgaggcgaat
    22861 ggtttcttcg cagatgattt tgttgagcgg attgtggtac ttgtcgattt cctcttccgg
    22921 cttacagtca acgacgttgt agtcgtagac gaagtacatg ttggtgccgt tctgatagct
    22981 atgagaggag tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact cggtacgaat
    23041 acggttaata acgctttcgt agatttcgtg aatgcagctc cagcagccgg acacttcagt
    23101 ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca ctttatccgg
    23161 tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc gctggcactg
    23221 cgggtagcgc gccacgattt cagcaatccc ttcacccgtc gccttcgcaa tgcgagggtt
    23281 accttcagcc ataaagatca gcacgcattt gccgtcggca aaatgggtga agtgttgggt
    23341 gccatcttca gcatcataca aacgagcaat cgacggacga tacccttcca ccatgatttc
    23401 acgcaggatg ttgaagccgg ttttcatatc ttccaggata tagccgtaga agagattgtt
    23461 ttccggcgta aatttgaaga ttttcactgt gacttcagtg atatagcaca gtgcaccttc
    23521 gttgccgatg atgatatgac gaatgtccgg gccagccgcg cgacgtggca cttttttaat
    23581 gcgtgtgacg gtgccatctg ccagcaccgc ttccagacca acgaccatat cttcgattgc
    23641 gccgtagagt gtggagaact gcccgatact gcgggtcgcc accaagccgc ccatctgcgc
    23701 cagcggcttt gactgcggag aatgccccgt ggtgtaacct ttttcgcgca atgcgttttc
    23761 cagcacctcc agcggaacgc cacattgcgc cgtggcctgc atattctcaa tatcaatatt
    23821 aatgatttga ttcatggcgg agccgtcgag caccaccgag ttttccacaa cggtttccag
    23881 tccaccttcg gtggcggaag caccggtacg tggcacaccg ttaattttgt gcgcattcat
    23941 aaaattcagc acacgggata cttgttctgt ggaaccgagt tttacgaccg ctgccggaat
    24001 cggcaaagta taaattccat gaatatccgg aaatttacga aaacggtcaa tactgttttt
    24061 ctttaatacc gtttcatcgg taataacgcg atctgcacca acaatttcct ttagctggtc
    24121 gacaatcgct acgcgagata aagacataat aattccttcc tgataataaa aatggaatag
    24181 ttattaataa gagaaaacag aaaccaccgt tatgaattag cgcactaaat aaccgccatc
    24241 aaccactaat aaatgtccgt tgacataatt cgatgccgga cttgcgagga atacggctgc
    24301 ccccattaaa tcctgagtat caccccaacg gtttgccgga atatgatcaa gaacacgctg
    24361 gttagtttct ggattactac gggtcgccag cgtaatatcg gttgcataat aaccaggagc
    24421 aataccattc acctgaatat tatattggcc taattcatcg caataggctt tggtgaatcc
    24481 ggcaagagca tgtttagtgg cagaataagc aggtgaccat tgaccaccta agtaagagaa
    24541 caatgaacag atattaatga ttttaccgct tttctgcggg atcataattt ttgccgcttc
    24601 gtagcttaac tcgaatgcgg cggtcaggtt cacgtcaatc atcgggtccc agtcggcacg
    24661 accaaagtcc agcaccttat tcagcttaca aataccggca ttattgacca gaatatcgac
    24721 ggtaccgaag cgctcacagc aggcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
    24781 atccacctgc ataaaatcaa cttcaacgcc ctgtttttca atcatttcct ttgtttcgcc
    24841 gttatctttg acgaaactag gaataaagac atttgcgcca gctttggcca acgccatggc
    24901 aaatgcctgg cctaaaccgc tattcccgcc ggtaacaatt gctgttttac ctttcaggga
    24961 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
    25021 taaggcaaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat
    25081 ctctggtaac tggagataat ttttagcata aggacataac tgctaatgtg atcgtaatca
    25141 cagtgtgatg ttcgtattgt caaataattt aaaaaagcag aagcgtgatt actctcacac
    25201 aacacctctg ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat
    25261 ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat
    25321 atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc
    25381 gttagtttcg acctggctta tttacgttat atttatcaaa ttcccatggc aaaatttatg
    25441 ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc ggccattatt
    25501 ttctatgccc ctagcggcgt tattgccgat aaattttcac atcgcaaaat gattacttcc
    25561 gcgatgatca ttaccggatt actgggtctg ttaatggcaa catatccacc gctgtgggta
    25621 atgctctgta ttcaggtcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg
    25681 attaaagccg catcgttgct gggcgatcat agcgagcaag ggaaaattat gggctggatg
    25741 gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct
    25801 cgctttgcac cggatgacag cgccagcctg aaaacggtca ttattatcta tagtgtggtt
    25861 tacattctgc tgggcatttt gtgttggttt tttgttagcg ataacaacaa cctacgcagt
    25921 gccaataacg aagaaaaaca gtcattccag cttagcgaca tcctggccgt tttgcgcatc
    25981 agcactacct ggtattgcag catggtgatt tttggtgtct tcaccatcta cgccattctg
    26041 agttactcca ccaactatct gaccgaaatg tacggcatgt cgctggtggc ggcgagctac
    26101 atggggattg tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg cggcattatc
    26161 accacctaca gtaaagtgaa atcccctacc cgcgtggtcc aaatcctttc catcatcggt
    26221 ttgctggcgt tgacagccct gctcgtcacg aactctaacc cgcaatcggt cgcgatgggg
    26281 attggcttga ttttattact gggattcacc tgttacgcct cacgcgggct gtactgggcc
    26341 tgccctggcg aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg
    26401 gtgattggat tcctgccgga tgtcttcgtt tacccgatta tcggctactg gcaagacacc
    26461 ctgcccgctg cagaagccta tcgcaacatg tggctaatgg gcatggcggc gcttggcatg
    26521 gtgatcatct ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca
    26581 atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca
    26641 tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta
    26701 atgtggtttg cgaaggcaaa ggcttattgc agccgatgca cacgccagat gccgataccg
    26761 cagaacatcc tgacgatgat ttatgggcat cactatgttt tgccagtcac gatttgatga
    26821 gccagtttgc cgggaataaa gaagatattg tcggcattgg cctgggctca atccgttgct
    26881 gccgtgcgct attgaaagcc gatggtacgc cagccgcgcc gttgattagc tggcaggatg
    26941 cacgcgttac acgcccttac gaacacacta atcctgacgt ggcctatgtc acctcttttt
    27001 cgggttatct gacacatcgc ttaaccggcg agtttaaaga caatatcgcc aactattttg
    27061 gtcagtggcc ggtggattat aagagctggg catggagcga agatgctacg gtaatggaaa
    27121 agtttaatat cccccgccag atgctttttg acgtgcaaat gccgggcacc attcttgggc
    27181 atatcacacc acaagctgcg ctggcgacac atttcccggc tgggctgccg gttgtttgta
    27241 ccaccagtga taaaccggta gaagctctgg gagccgggtt actggatgat gaaacggcgg
    27301 taatttctct tggcacctat atcgcgttga tgatgaacgg caaagcactg ccgaaagatc
    27361 cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg
    27421 gtattcgcaa aggcatgtgg acggtgagct ggctgcgcga catgttaggc gagtcgttaa
    27481 ttcaggatgc caaagcacag gatctttcac cggaagattt gctcaacaaa aaagcgtctt
    27541 gcgtgccgcc tggctgtaat ggtctgatga cggtgctgga ctggctgacc aatccgtggg
    27601 aaccgtacaa acgcgggatt atgatcggtt ttgattccag catggattac gcatggatat
    27661 atcgttcgat actggaaagc gtggcgctga cgctgaagaa taattacgac aatatgtgtc
    27721 atgaaatgaa tcactttgca aagcatgtga tcattactgg cggcggttcg aacagcgatc
    27781 tgtttatgca gatttttgcc gacgtgttca accttccggc acgccgaaac gccattaacg
    27841 gttgtgcaag tctgggggct gcgatcaata cggcggtagg tctggggcta tacccggatt
    27901 acacaacggc tgtcgataaa atggttcgcg tgaaagatat cttcataccg attgagagca
    27961 atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc aaacatactg
    28021 atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat gcggattcga
    28081 tccagagctg gtcgaatgcg taagtgagat aaacagtgcc gggtagatga ttaatcccgg
    28141 catattcacg tttccactac aaaataccgg gtaaccactg ctgaatattg ttctctcctg
    28201 agcagcgttt atgctttaat tgctaaaccg atttaattag tttagcaatt agtatattgg
    28261 agcctttatg agcacaagag tatgggtact cggtgatgcg gttgttgatt tattacccga
    28321 aagccagggg agactactac agtgtcctgg tggggcgcct gctaatgttg cagtcggtat
    28381 cgcaaggctg ggggggaaaa gtgcctttat tggcaaagtt ggcgatgatc ctttcggtcg
    28441 ctttatgtat caaacactga gtacagaaaa tgttgataca cgttatatgt ctcttgatcc
    28501 tcaacatcgc acctcaattg tggctgtagg acttgatgag caaggagaaa gaaactttac
    28561 ctttatggta cgcccaagtg ccgatctttt tttacaacct gatgaccttc ctgcatttgg
    28621 gccgggtgaa tggctccatc tttgttccat tgcgctcagt gcagaacctt cccgaagtac
    28681 cgcatttctg gctatggaga aaatacgtca ggctggcgga aacatcagtt ttgatcccaa
    28741 tatccgcagc gatctctggc agagtgaagc gctgttaagg aaataccttg atcgtgcgct
    28801 ttcgctggcg aatatcgcta aattgtccga agaagagttg ttattcatca gtgacgaaag
    28861 ccaggttcag caaggcgcat attcattagt acaacgttat ccgttgactt tattgcttat
    28921 tacacaagga aaaaatggcg tacttgtgta ttttcagggg cagtttatcc actatcccgc
    28981 caaacctgtt tctgtcgtcg atacgaccgg ggcaggagat gcttttgtcg ctggattact
    29041 tgccggtctg gctgattctg gaataccaac aaataccaga cagcttgaac gaatcattgc
    29101 acaagctcag atttgtggtg ctctggcgac cacggctaaa ggcgcgatga ccgccttacc
    29161 ccgacaacac gatctccctt cacaatagtg atcctgctgt tgtatctgta acccgatttg
    29221 tcgtgcctgt cacatttact aaatcggttt agcgaaaata attgctctgg ctaaaaagtc
    29281 agctttatta tcaacaccta aaccggttta gcattttagc aatttttata attctctgat
    29341 gggacgcgac aaattatgta taaaaaaaca actttggcag tgttaattgc tttgctgacc
    29401 ggtgctacaa cggtacatgc gcaaacggat attagcagta tcgaatctcg actggcggcg
    29461 ttggaacaac gtttaaaaaa tgcggaatcc cgcgcccagg cggcagaagc aagggccaaa
    29521 acagctgaat tacaggttca gaaactggct gaaacacaac aacaaaatca gctaacaact
    29581 caagaagtag cacagagaac agttcagctc gaacagaaat ccgcagaaaa cagtggtttt
    29641 gagtttcatg gctatgcccg ttccgggtta ctgatgaatg atgccgcttc cagtagcaaa
    29701 agtgggccgt atctgactcc cgcaggtgaa actggtggag ctgttggccg tctgggaaat
    29761 gaagccgata cctatgtcga gttaaatgta gaacataaac aaacactgga taacggtgcg
    29821 accacacgct ttaaagcaat gttggctgac ggacaaagag attacaacga ctggactggc
    29881 ggctccagta acctgaatat ccgacaggct tttgccgaac tgggcgcatt accaagtttt
    29941 accggagcat tcaaagacag tactgtctgg gctggtaaac gctttgatcg cgacaatttt
    30001 gatattcact ggttagactc cgatgtcgta tttttagcgg gaacgggcgg cggtatctat
    30061 gacgtaaaat ggaacgagac attccgcagt aacttttctc tctacggacg taatttcggc
    30121 gatcttgatg atatcgacaa taacgttcag aactacatcc tcaccatgaa tcattatgca
    30181 ggccccttcc agttgatggt tagcggatta cgggcaaaag ataatgatga tcgaaaagat
    30241 gccaatggtg atctcattca aactgatgct gcaaatactg gcgtacatgc gttagttggt
    30301 ctgcacaatg acactttcta tggcctgcgt gaagggacgg caaaaacagc actgctatat
    30361 ggccatggcc tgggtgcgga agtcaaaggg attggctccg atggcgctct gctgtctgag
    30421 gcgaatacct ggcgcttcgc atcttacggc acaacacctc tgggaagcgg ttggtatgtt
    30481 gcgccagcaa ttctcgcaca aagcagtaaa gatcgttacg tcaaaggcga tagctacgaa
    30541 tgggtgacct tcaatacacg tctgatcaaa gaggtaacac agaattttgc tctggccttt
    30601 gagggtagct atcaatatat ggatctgaag ccaaaggggt atcaaaacca caacgccgta
    30661 aacggcagct tctataaact cacctttgct ccaactctaa aagctaacga tatcaataat
    30721 ttctttagcc gtccggagct tcgcctgttt gccacctgga tggactggag cagcaaactt
    30781 gatgattttg ccagcaatga tgctttcggc agcagtggtt tcaatactgg cggagagtgg
    30841 aattttggtg tccaaatgga aacctggttt taataccaga cagcctcacg gatgtgaggc
    30901 ttttactgaa ataacaatga attagagggt tatatggatt tcgataaaat cgcccaatcg
    30961 ttgcttcctt tattaggtgg taaggagaac atcgccagtg cagcacattg cgccactcgc
    31021 ctacggctgg tactggttga cgacacactt gccgatcaac atgccattgg ccagattgat
    31081 ggagtgaaag gttgttttcg taattcaggg caaatgcaaa tcatctttgg caccggcgtg
    31141 gttaataaag tctatgctgc ctttattcag gttgcaggta ttagcgaatc cagtaaagca
    31201 gataccgctc gactcgccgc tcaaaaactc aatccttttc agcgaatagc acggctactt
    31261 tctaatattt ttgtccccat cattccagcc attgttgctt ctggtttatt aatggggctt
    31321 ctgggaatgg tgaaaacata tggctgggtg aatgccgata atgcgattta tatcctgctg
    31381 gatatgtgca gttcagccgc atttattatt ctccccatcc ttattggctt tactgctgct
    31441 cgtgaatttg gtggaaatcc ttatcttggc gcgacattag gagggattct gactcacccg
    31501 gcactcacaa atgcctgggg cgttgctgcg ggatttcaga caatgaactt cttcggtttt
    31561 gaaatcgcca tgattggcta tcagggaacg gttttccccg tgcttctggc agtatggttt
    31621 atgagcatag tggaaaaaca gttacgcagg tttatccctg atgctctgga tctcattctg
    31681 acgccatttc tgactgtcgt catttctggc tttatcgctc ttttgattat tggtccggca
    31741 ggacgagcgt taggtgacgg tatctctttc gttctcagca cgctgattgc acacgcaggc
    31801 tggttagcag ggttgttatt cggcgggcta tattcagcga ttgttattac gggtattcat
    31861 catagcttcc acgcaattga agcaggactt ttaggtaacc ctgcaatcgg tgttaatttc
    31921 ctgctgccta tttgggcaat ggcaaacgtt gcgcaaggcg gtgcatgtct ggcggtatgg
    31981 tttaaaacca aagatacaaa aattaaagca atcaccctac cctcggcctt ttccgcaatg
    32041 ttagggatta ctgaagccgc tatttttggt atcaaccttc gttttgttaa accttttatt
    32101 gcggccttaa ttggtggcgc tgccggcggt gcctgggttg tctctgttca cgtttatatg
    32161 actgccgttg gtctgaccgc aatacccggc atggcaatcg ttcagccaac atcgttggtt
    32221 aattatatta ttggaatggt gattgctttc gctgtcgctt ttagcctctc tttattactc
    32281 aaatacaaaa cggacgagga gtaatgtcga tgccttcaca aatgcccgcg attttacagg
    32341 cagtgatgaa aggtcagtcc aaagcactgg cagatgccca ctatccttgt tggcatctcg
    32401 ctccagtcac cggactaatg aacgatccaa atggcttttg ttggtctgga ggccgctatc
    32461 atctgtttta tcaatggaat ccactggctt gtgatcataa gtacaaatgt tgggggcact
    32521 ggagttcgac ggatttattg cactggcagc atgaaccatt agcgctaatg cccgataaag
    32581 agtacgaccg taatggttgt tactcaggaa gcgcggttaa taatcagggc gttctcacac
    32641 tttgctatac cggtaatgtt aagtttgatg atggcagcag aacagcatgg caatgtctgg
    32701 ccaccgaaaa taaccagggc gggtttgata aactaggccc tgtcataccg ttacctgatg
    32761 gttacaccgg acatgttcgt gatccaaaag tctggaagca caacagccaa tggtacatgg
    32821 tgcttggcgc gcaagataaa gagaaacggg ggaaagtact gctgtattcc tctgtcgatc
    32881 tctatacctg gtcttttcat ggagaaattg ctggtaatgg tctgaatgaa atagataacg
    32941 caggatacat gtgggaatgt cctgatcttt tcgcccttga tggtgaatac attctcctct
    33001 gctgccccca gggaatggcg cgcgaacatg agcgctatct gaatacttat ccctgcgcct
    33061 ggctacatgg acagtttgat tacgagacag gcaagtttac gcatggcgct ttctcagagc
    33121 tggatgcggg atttgaattc tatgcaccac agacgatgga agccccggac gggcgacgat
    33181 tgctggtcgg ctggatgggg gttcctgatg gcgaagagat gttgcaacca accagaaagc
    33241 atcattggca gcatcaaatg acctgttttc gcgaactctc attccagaag ggaaaattgt
    33301 ttcagatgcc aatcagggaa ctcgcgcaac tgcgtgaagc tgaacacttc tggcaaggaa
    33361 aagcggatca tgcgccgcat gtcgaaattg aacgcctgga aatggacatc attccgtcgg
    33421 gtgagctgta tctgaatttt ggcaatgcgc tggcactgca tcttaatgac gatggtattc
    33481 aactacagag gagaagcctt gcagggcaag agaaactaac tcgttactgg cgaggaagcg
    33541 taacgtcact gaagattctc tgtgacagtt ccagcgtaga gatattcatc aataatggtg
    33601 aaggtgtaat gagtaatcgt tattttcccc atcatccagc ttcgctgata ttacagggtg
    33661 agtctgacgt cacgttacgc tactggtcgc tacgcgcctg catggtagaa tgagggtttt
    33721 gaattacgga tcctcgtcgt gagaaaaacc aaacgcgtta ctattaaaga cattgctgaa
    33781 ctggcgggag tgtcgaaagc aaccgccagt ctggttctca atggtcgtgg caaagagtta
    33841 cgtgttgcgc aggaaacgcg tgagcgtgtg ctgagtattg cccaacagca gcattatcag
    33901 cccagcattc atgcccgctc attacgggac aaccgcagtc ataccattgg tctcgtcgtt
    33961 cctgaaatca ctaactacgg atttgcagta ttttcccatg aactggaaac attgtgtcgg
    34021 gaagcgggcg tccaactcct catatcctgt actgatgaaa atccttcaca ggaaacaatg
    34081 gtggtgaaca atatgattgc ccgccaggtt gatgggttaa tcgtcgcatc cagtatgctg
    34141 catgataatg actatcaaaa acttagcgag caattacctg ttgtgttgtt cgatcgccac
    34201 atgaacggca gcacattgcc attggtcatt actgactctg tttctcccac cgccgctctg
    34261 gtggcagata ttgcacggag tcacccggat gaattctatt ttctgggggg acaaccccgt
    34321 ttgtcaccga ctcgtgatcg actggaagga tttacacaag gcttgcagca agccggagta
    34381 acgctacaac ctgaatggat tattcacggt aattatcatc cgagctcagg ctatgaaatg
    34441 tttgccgcgc tatgcgctcg tttaggacgt ccccctaaag ccttattcac tgccgcttgt
    34501 ggattactgg aaggcgttct gcgctatatg agccaatata atttactcga cagtaaaatc
    34561 cacctggcga gttttgatga tcattacttg tatgattcac tttcggtaag aatcgacaca
    34621 atacaacagg ataatcgcca acttgctttt cactgctttg aacttatttc gcaactgatt
    34681 gaaggtgaaa cgccatcccc attacagcgt tatcttcctg ccagtctaca aaaacgctac
    34741 cgataatccc gtcactatct tgcatcgccc ctgtaaaacg gggcgattat tttcatcctg
    34801 aacgttaaat atcaatgatg cataatcaaa tcaggcaata tttaaatatt tatgcgtttg
    34861 catcgacaaa cgccagttgc gcgcaatgca ggtatcgata cataaacgcg tagcatcttc
    34921 cttctggcta attggctgca gtgcaatgac tcgcggttta tcatcggtca gcgtcgcaag
    34981 caattcatcc agtgcttcga tatcacgtac gcgtcccact gggtgtttga tttcgttggc
    35041 tcgctccagt gcctgtgaca acacttcata gccaccgcgc atattcagct ttggcgacac
    35101 ggtgacccag gtattcggtg tacagcgtac ttcatgagta ccactggttt ctatctggca
    35161 gctaaaaccg ttcttttcga gcaggtcagt cagcggcagc aaatcatgaa tgcaaggctc
    35221 accacccgta atcaccacgt gacgcgcggt gtatccctga cggccaatga cagccagcaa
    35281 atcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa
    35341 aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca
    35401 gccaaccgga catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc
    35461 accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg
    35521 gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa cattctgtta
    35581 ccggcaggtt taaagaaaaa tgcgaaacat atcgttaata ataaaaggga gtgacgtatt
    35641 atgtcagaaa gaaataaagt aaagg
//
LOCUS       DAPQZD010000034        34771 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPQZD010000034 DAPQZD010000000
VERSION     DAPQZD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.034"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1970
                     /locus_tag="RO454_001614"
     CDS             <1..1970
                     /locus_tag="RO454_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDY0933461.1"
                     /translation="AGSQAGTVRVTAKVQGKTFTLSIKQTATTEPDSEVSAVLTAAPA
                     EQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYSATVTSK
                     RAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRNMVTVSL
                     ADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVITAHAGS
                     QMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNASVNGQKI
                     QLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGSVKLTFV
                     PDVASAELNMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTGASLTSS
                     NLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVLEITSSA
                     SSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTTDENGQA
                     TVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPGYETNDGSPV
                     GIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEFSTYESG
                     YHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
     gene            complement(2007..3050)
                     /locus_tag="RO454_001615"
     CDS             complement(2007..3050)
                     /locus_tag="RO454_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0933462.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            3400..3501
                     /locus_tag="RO454_001616"
     CDS             3400..3501
                     /locus_tag="RO454_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933463.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHLASDLCGGEA"
     gene            3498..3953
                     /gene="ybcN"
                     /locus_tag="RO454_001617"
     CDS             3498..3953
                     /gene="ybcN"
                     /locus_tag="RO454_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDY0933464.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            3953..4123
                     /locus_tag="RO454_001618"
     CDS             3953..4123
                     /locus_tag="RO454_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933465.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            4116..4406
                     /locus_tag="RO454_001619"
     CDS             4116..4406
                     /locus_tag="RO454_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDY0933466.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            4403..4765
                     /locus_tag="RO454_001620"
     CDS             4403..4765
                     /locus_tag="RO454_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDY0933467.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            4762..4902
                     /locus_tag="RO454_001621"
     CDS             4762..4902
                     /locus_tag="RO454_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDY0933468.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPSSVLTDYRILKNYAKTLKGA
                     AA"
     gene            4899..5588
                     /locus_tag="RO454_001622"
     CDS             4899..5588
                     /locus_tag="RO454_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDY0933469.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQLYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     IFSQQDIAKLD"
     gene            5910..6215
                     /locus_tag="RO454_001623"
     CDS             5910..6215
                     /locus_tag="RO454_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDY0933470.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            6202..6678
                     /locus_tag="RO454_001624"
     CDS             6202..6678
                     /locus_tag="RO454_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDY0933471.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            6895..7077
                     /gene="rzoD"
                     /locus_tag="RO454_001625"
     CDS             6895..7077
                     /gene="rzoD"
                     /locus_tag="RO454_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDY0933472.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(7168..7461)
                     /gene="iss"
                     /locus_tag="RO454_001626"
     CDS             complement(7168..7461)
                     /gene="iss"
                     /locus_tag="RO454_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDY0933473.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            7942..8268
                     /locus_tag="RO454_001627"
     CDS             7942..8268
                     /locus_tag="RO454_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HDY0933474.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            8475..8657
                     /locus_tag="RO454_001628"
     CDS             8475..8657
                     /locus_tag="RO454_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933475.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            9221..9766
                     /locus_tag="RO454_001629"
     CDS             9221..9766
                     /locus_tag="RO454_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDY0933476.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     DVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            9741..11666
                     /locus_tag="RO454_001630"
     CDS             9741..11666
                     /locus_tag="RO454_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDY0933477.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHCMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLHVDEAINKTYT
                     RRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            11663..11869
                     /locus_tag="RO454_001631"
     CDS             11663..11869
                     /locus_tag="RO454_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDY0933478.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            11866..13467
                     /locus_tag="RO454_001632"
     CDS             11866..13467
                     /locus_tag="RO454_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0933479.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            13448..14767
                     /locus_tag="RO454_001633"
     CDS             13448..14767
                     /locus_tag="RO454_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDY0933480.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNLASDAVNDLLNTPV"
     gene            14777..15109
                     /locus_tag="RO454_001634"
     CDS             14777..15109
                     /locus_tag="RO454_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDY0933481.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            15165..16190
                     /locus_tag="RO454_001635"
     CDS             15165..16190
                     /locus_tag="RO454_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDY0933482.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLV
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQLEGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            16232..16630
                     /locus_tag="RO454_001636"
     CDS             16232..16630
                     /locus_tag="RO454_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDY0933483.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVTLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            16642..16995
                     /locus_tag="RO454_001637"
     CDS             16642..16995
                     /locus_tag="RO454_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDY0933484.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            17007..17585
                     /locus_tag="RO454_001638"
     CDS             17007..17585
                     /locus_tag="RO454_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933485.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGDSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            17582..17977
                     /locus_tag="RO454_001639"
     CDS             17582..17977
                     /locus_tag="RO454_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933486.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            17985..18725
                     /locus_tag="RO454_001640"
     CDS             17985..18725
                     /locus_tag="RO454_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933487.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELSAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            18741..19163
                     /locus_tag="RO454_001641"
     CDS             18741..19163
                     /locus_tag="RO454_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDY0933488.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKRQAEPAGSDSN
                     RQVTVEDVIRTGAFLVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            19145..19579
                     /locus_tag="RO454_001642"
     CDS             19145..19579
                     /locus_tag="RO454_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDY0933489.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            19572..22133
                     /locus_tag="RO454_001643"
     CDS             19572..22133
                     /locus_tag="RO454_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDY0933490.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYAARQEELNKALKDGKILQADYNTLMAAAKKDYETT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSVQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            22130..22459
                     /locus_tag="RO454_001644"
     CDS             22130..22459
                     /locus_tag="RO454_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933491.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWQQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            22459..23157
                     /locus_tag="RO454_001645"
     CDS             22459..23157
                     /locus_tag="RO454_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDY0933492.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            23162..23905
                     /locus_tag="RO454_001646"
     CDS             23162..23905
                     /locus_tag="RO454_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933493.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPKGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            23842..24444
                     /locus_tag="RO454_001647"
     CDS             23842..24444
                     /locus_tag="RO454_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDY0933494.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            24505..27987
                     /locus_tag="RO454_001648"
     CDS             24505..27987
                     /locus_tag="RO454_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDY0933495.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLLNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKETIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVEASARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PTFSLTPDGRLSARNADISGNVNANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVG
                     KAFPRNGSYASGTITVTVYDDQAFDRQIVVPPVLFRGGKHENFNSNNQQSYWYSTCKL
                     QVLKNGQEIFQQPATDVSRVFSSVIDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLL
                     VVVMKKSTAGISIS"
     gene            28046..30067
                     /locus_tag="RO454_001649"
     CDS             28046..30067
                     /locus_tag="RO454_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDY0933496.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVALLVEGFPPSHAGTITVYEDSKPGTLNDFLGAATEDDVCPEA
                     LYRFEKMVEEAARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPCANIVPPLPDVWIPFNDSLDMITGFSP
                     SYKKIVIGDDEITMPGDKIVKFKRASTATYINKSGQLKLAEVDEPRFERDGLLIEGQR
                     TNYLRNSNKPDSWTVHSALNKTFGTDKQGFNYATVTPTESIVGTTGGYTVHGVVAADR
                     FPLASGECFTFSCRVKGAKARCRLRVSVIIGGTDTFSADSYLDLDTRIATVSGNTSLI
                     TAKAEQQGEWTYYEATYTANTDIDTVNCAFYMTNKISNETFYDDSTLTMTTPQIELGN
                     TASSFIVTTMPTTRASDVVTIPSQNNLSTRPFTVLCEVSRNWSTPPNVAPRIFDVGGH
                     SIDDNYLSLGFVATGKISANVGMVQPQIASDGERFIVGVRAKSDLSVNAICNGNYTTN
                     LNGKIFGVTATSYRFGGQTAAGTRHLFGHIRNFRVWFKELNDRQIKEAV"
     gene            30064..30342
                     /locus_tag="RO454_001650"
     CDS             30064..30342
                     /locus_tag="RO454_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933497.1"
                     /translation="MKDLTLKFPGNREFKSFLSSLDWEEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCVRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            30355..30648
                     /locus_tag="RO454_001651"
     CDS             30355..30648
                     /locus_tag="RO454_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933498.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(30740..31597)
                     /gene="sitD"
                     /locus_tag="RO454_001652"
     CDS             complement(30740..31597)
                     /gene="sitD"
                     /locus_tag="RO454_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDY0933499.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(31594..32451)
                     /gene="sitC"
                     /locus_tag="RO454_001653"
     CDS             complement(31594..32451)
                     /gene="sitC"
                     /locus_tag="RO454_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDY0933500.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(32448..33275)
                     /gene="sitB"
                     /locus_tag="RO454_001654"
     CDS             complement(32448..33275)
                     /gene="sitB"
                     /locus_tag="RO454_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDY0933501.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEDVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(33275..34189)
                     /gene="sitA"
                     /locus_tag="RO454_001655"
     CDS             complement(33275..34189)
                     /gene="sitA"
                     /locus_tag="RO454_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDY0933502.1"
                     /translation="MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         8652 a         8328 c         9920 g         7871 t
ORIGIN      
        1 ctgctggttc gcaggctggt acggtacgtg taactgcaaa agttcaggga aaaacattta
       61 ctctcagtat taagcaaaca gcaacaacag aacctgattc tgaggtcagt gctgttttaa
      121 ccgctgcacc agcagaacag gtagttggtt acaatatcaa tctacaactg gcagtgaagg
      181 attcacaagg aaatgcaatc actggtgata acactctgag cttttatgct cttaaccagg
      241 cagaaggagt cgagtttggg actgtaacgg aaaaagacgg tgtctatagc gcgacagtaa
      301 cttcaaaacg agctggtaag attactatcg gtgttaaatc tgatagtcat gatttctcag
      361 gtgttaagaa agaaatcacc tttattgagg accggcagca ggttacattc tctcagtttg
      421 aagccagcca aaataatgca ttggctgatg gaaaacagag aaatatggta actgtaagcc
      481 ttgcggaccg tttcggcaat gttgttcctg gttatgctgt cacattgtct ttaccagcag
      541 ggataactca agttggtggt gaacatgctg tttcaactga cgaaaatggt aatgctattt
      601 ttgcgttaat aagctccact ccggggtctt acgtgattac agctcatgct ggttctcaga
      661 tgtctactga gttaacagtg acctttgcat caaatatgac cggagcttca ctgtcactga
      721 cgcctgaaag cagtagttta atttccaata ttcctgctaa tggtaaggat gcagcggtat
      781 tagatgtgca gctgacaaat acgaatgcgt cggtcaatgg gcaaaagatt cagctcatca
      841 cctcatctga aggattatct gtaccaacaa atatcgtgac tgattcaaca gggcatgttt
      901 ctgttccgct tacaacggtg aaagctggtg agtacacggt gactgcacgg gtgactgacg
      961 gttcccatag cgtggaatct ggcagcgtta agttaacttt tgtgcccgat gttgcttctg
     1021 cagaactgaa tatgagtgta tcaaaacagg agattgtggc cgatggcagt gaaaacgcaa
     1081 cagtaaacat tcagttggtt gatgcgaata acaatgcgtt tacaggcgag gtggatttaa
     1141 cgattacacc atcgacggga gcatcattga ccagtagtaa tctgcaactg gatgctcatg
     1201 gacaggcaac aacgcagttc actgcatcga aatcaggcca gtacacaatt caggccgagt
     1261 atgttctgga tgggaaacgc ataaccgcaa gccagaatat tgatgccgtg acggatgtga
     1321 agggggctgt gttggagatc acatcatctg catcttcggc tgtagtgagc gataccaaca
     1381 atctggtatt tacgttactg ttgaaaagta agtcaggcga agcattaagt ggtcgagcat
     1441 tgaatgttaa gacatctggg ccgtctaaat atggtgcgtt ggtggttgat aaaaccacag
     1501 taacgactga tgagaacggt caggctacgg taagcgtaca tggtcgaacg gttggttcgt
     1561 ataaattaac ggcgacctta aacgaacttg gcagtgatgt tagcgcggtg aaatcattct
     1621 ccctgtacgc tgatgacgca aatggagtat tgactctaac aaaagatcct ggttatgaga
     1681 caaacgatgg atctccagta ggtatttatg ctcgatttgt cgatcatttt ggaaacccat
     1741 tatctggtac ggttgagttt tctgctggta gtgaagatag tccagactct caacgggtca
     1801 aaatggagcc tgctacagta acgttacact ggacaggtaa tgcagcttcg gaattcagca
     1861 cctatgaaag tggttatcac tggattaagg ccaaaataac gaatagcaaa aatacatatg
     1921 agaaaacaat aaggacttat gttgtaaaac tacctgaaaa ggatagttaa caaaattgaa
     1981 tggggagggc ttagccctcc ctctttttac aggttagcgt ttgggttttg ttgttgggga
     2041 gctgttttta taggcgctcc caggttttta gtaaaccact caacgactct gtttataata
     2101 attggctgat accatgtctt atcgccatgc tcggcccctt ctaccagcac gtactcagcg
     2161 ttatcaccgt tcttcttcaa catcctgaat agtttggcgc tttgctcagg tgaaaccaga
     2221 gtgtctttgc taccatgcat aataagaaat gggggtttta ctcctttcat atgtccgatt
     2281 ggactggcgt ttagcgcttt ttcttttgac gctgtgatgg gggctcccgc aaaatttctg
     2341 aatgcagggc cattgatcat taaggcttcg gttacggcag gagagcgatg aacctcctgc
     2401 actgattcag ggaacccctc gccaatattc aacaagtcag aaatcccata aagtgtggca
     2461 actgcctgaa catctgagga ttgctgaaga aagtcacctt tatcaaaggt tttgtcacca
     2521 tttgtagttc ccatcatctg ggcaagccat ccaccggcag agtcacccag aactccgatt
     2581 ctttgaggat caatcccata attgctggca tgctctctca ggtaacgtat tgcagctttt
     2641 ccatcctcaa ctggtgctgg aaatgtatca ggaattgttc tgtattctac agcggccaca
     2701 acaaaaccag cttctgccag agccattctc atttcaataa atttgtcatg ttcagaagac
     2761 atgaatccgc cgccgggata ataaataatg gctggtttta aatcatttgt tcgcgggaca
     2821 agaactgaca tgtgaagctg tctgacagaa cgagttcctt ttatctggga ataaacaaca
     2881 tcaccaatga gatcgacctg gtttctggtc ggtgaaacac taatgatatc tgcacccggg
     2941 gtgtaaccag gaagattagt ctggacaggt gttgcacaac tcccgaccga catggccatt
     3001 gacataccat acaatagtgt tgcagatgat aatatcttgt taattttcat gttttacatg
     3061 ccttgactta tcggatgaaa ttctcttact aaatttaacg gcagtttaat agccgtacaa
     3121 ctcgtggtag ccagggaaat atcccagtcg tggttaaggt tgtacttgac ggctattatt
     3181 tcaacaatgc ttaaagtgag agcttaaggt tatgcgtatg ttttacagtc cgagtagatt
     3241 ctgaccaata gcttctttgt ataattctgt cagaaataaa tcttggcttg catgaagttt
     3301 gagaccttat cttgtttgat tatgaataat caattcgcca taattgtatc accggagcct
     3361 gaacaactcc ggtgacttct gcgctaaacg gggacgttta tgcgcacaca caatccaaac
     3421 tctcttctcc cttcacagat gcagaaatgc acctgcgttt ttttgcatct agcgtctgac
     3481 ctctgtggag gtgaagcgtg aacctaccac aagatggcat caaactgcat cgcggtaact
     3541 tcatcgctat cggccagcag atccagcctt atctggagaa cggaaaatgc tttcgcatgg
     3601 tgcttaaacc gtggcgtgag aaacgcagtc tttcccagaa tgcactcagc cacatgtggt
     3661 acagcgaaat cagtgaatac ctcatcagca gggggaaatc gttcgctacc gcagcatggg
     3721 taaaagatgc tctcaaacac acatacctcg gttatgaaac caaggacctg gttgatgtcg
     3781 taaccggcga aatcactact atccagtcgt tacgccatac ctccgatctc gataccggag
     3841 agatgtatgt cttcctgtgt aaggttgaag cctgggcggt gaatattggc tgccacctga
     3901 ctattccgca gagctgcgag ttccagctgc tgcgcgacaa gcaggaggcg taatggctac
     3961 accgcttatt cgtgtcatga acggacacat ctacagagta ccaaatcgtc gtaagcgtaa
     4021 gcctgagctg aaaccatccg aaataccaac actgctcgga tataccgcca gcctggttga
     4081 taaaaaatgg ttgcgactgg cagcaaggag gaatcatggc tgacttgaga aaagcagcgc
     4141 gtggtcggga atgccaggta agaatccctg gcgtatgtaa tggcaatcct gaaacgtcag
     4201 tactggcaca tatccggctg gctggattgt gcggtaccgg tatcaaaccg ccagacctga
     4261 ttgccaccat tgcatgttct gcctgccacg acgaaatcga ccgtcgcacg cattttgttg
     4321 acgctggata tgcaaaagaa tgtgcgctgg aaggtatggc gagaacacag gttatctggc
     4381 tgaaagaggg ggtaattaag gcgtgaatac ttactgcatc acactaccct ggccgccgag
     4441 caataatcgc tattaccgcc ataatcgcgg acgcacgcat atcagcgcag aggggcaggc
     4501 ataccgcgat aatgtcgccc gaatcattaa aaacgcaatg ctggatatcg gcctggctat
     4561 tcctgtgaaa atcagcattg agtgccacat gccggatcgc cgtcgccgtg accttgataa
     4621 tctgcaaaag gccgcttttg acgcactcac caaagcaggt ttctggctgg atgatgctca
     4681 ggtcgttgat taccgcgttg tgaagatgcc cgttaccaaa ggtgggaagc tggagctgac
     4741 tatcactgaa ctgggagatg aatgatgttt gagtcttata tggcagaacg tcttcgccgc
     4801 cgctgggtgc gcctgcgctt atatcgtttt cctagttctg ttttgaccga ttaccggata
     4861 ctgaagaatt acgccaaaac actgaaagga gctgccgcat gaatacccaa tatttacagt
     4921 atgtccgcga gcaactcatt gtggctaccg ctgatttgag cggagcaacg aaaggacagc
     4981 ttgaagcctg gctggagcat gcacaatttg atactggtac atacaaacga aagaagccgc
     5041 gcattctgga tgaggtaact ggcaggatga ttacgctgga taatccgccg atttccggta
     5101 agcagttgta cgcaaaaggt tcatccattg cactggtcag ccaggttgag ttctcaacct
     5161 cgtcatggcg ccgcgcggtt ctgtctctcg aagaacatca gaaagcgtgg ttgctgtgga
     5221 gttacagcga aagtgttcgc tgggaacatc aggtcaccat aacgcagtgg gcatggagcg
     5281 agtttaagac tctgttgggt accaggaaaa ttgcaggtaa aacactggaa cgtttgaaga
     5341 agttgatctg gctggcggca caggatgtga agaacgagct ggcagggcgt aagacctatg
     5401 aataccagga gctggcatca ctggtgggag tgacatcaaa aaactggtct gagacattta
     5461 ctgaacgctg ggttgcaatg aagcacattt ttctacagct tgatagccaa gctttattgc
     5521 ttttaacgaa aacacgttca aaacaaaaga ccatattttc acagcaagat attgcaaaac
     5581 tggattaaaa atcatatatt ttatgtaaat ctgatatttt gccaatgttg tacgcactgg
     5641 cagtaatcca aattcaagcc cgaggtttaa agccttgggc ttttctgttt ctgaacggtg
     5701 agtagccttc caacctaccc cagccagggt gtcttcagct gttgagttga tattgcttaa
     5761 ccctctgttg ccagctacat gctggctttt ttattccagg cttgcgggga gcatcaactc
     5821 cgtgctttgt cgttaaatta ccccgtgagc ctgatttctg acatttaacg tcccggcctt
     5881 ttgtcggcgg cgaaacattg gctattcata tgcacgaaaa agagagcctt gccggagcgt
     5941 tctggctcgt tttgctgatc atcgcaggtt ggggcggtct ggtccgctac ctgatagatg
     6001 tgaagcagag taaagcaacg tggagttgga taaatgctct ggctcaaata gtggtatcag
     6061 gattcaccgg tgttattggt ggcctgatca gcatcgaaag tggattcagt atttacatga
     6121 ttctcgcgac agcggggatt agtggtgcga tgggttcggt tgcactgacg tacttctggg
     6181 aacgactgac aggggtgaaa aatgcaaaat cttaatcctc agcgtaaagc cttcctcgat
     6241 atgttggcgt ggtcagaagg aacggataac gggcgacaac cgacacgtaa ccacggttat
     6301 gatgttattg ttggcggcga actgtttact gattactccg atcaccctcg caaacttgtc
     6361 acgctacacc ccaaactcaa atcaacagcc gccggacgtt atcagcttct ttcacgctgg
     6421 tgggatgctt accgtaaaca gcttggtttg aaagacttct cccccaaaag ccaggacgca
     6481 gtggcattac agcagattaa agagcgtggt gctttaccga tgattgatcg cggtgatatc
     6541 cgtcaggcaa tcgaccgttg cagcaatatc tgggcttcac tgccgggcgc tggttatggt
     6601 cagttcgagc ataaggctga cagcctgatt gcaaaattca aagaagctgg cggaacgctc
     6661 agagagagtg aggtatgagc agaataaccg cgattatctc cgctctggtt atctgcgtca
     6721 tcgtctgcct ttcatgggcg gttaatcatt accgcgataa cgcgattacc tacaaagccc
     6781 agcgcgacaa aaatgccaga gaactgacgc tggcgaacgc ggcaattact gacatacaga
     6841 tgcgtcagcg tgatgttgct gcgctcgatg caaaatacac gaaggagtta gctgatgcga
     6901 aagctgaaaa tgatgctctg cgtgatgatg ttgccgctgg tcgtcgtcgg ttgcacatca
     6961 aagcagtctg tcagtcagtg cgtgaagcca ccaccgcctc cggcgtggat aatgcagcct
     7021 ccccccgact ggcagacacc gctgaacggg attatttcac cctcagagag aggctgatca
     7081 ctatgcaaaa acaactggaa ggaacccaga agtatattaa tgagcagtgc agatagagct
     7141 gcccatatcg atgggcaact catgcaatta ttgtgagcaa tacacacgcg cttccagcgg
     7201 agtataaatg cctaaagtaa taaaaccgag caatccattt acgaatgttt gctgggtttc
     7261 tgttttaaca acattttctg caccaccaca aatttttgct gcatcgacag ttttcttctg
     7321 cccaattccc gaaacgaaga aatgatgggt gatggtttcc tttggtgtta ctgctgtagg
     7381 tttgtttcca acagtaaacg tctgttgagc acatcctgta ataagcattg ccagagcggc
     7441 agaaaacaac atttttttca tcttattatc ctgcattgtt aaaaacggca gaatcctatg
     7501 tgacaacaat taaacgatag ttaaatggat tgatgaaaat taaaactaca caggtgggct
     7561 cagactattg gaggaagttg gggacactca gaatcctgtg gaatgaaata aaccggtcta
     7621 tccgtccatt acccttttag ctgcgctgta tcgtcgccgt attcccgcat taaccatgac
     7681 cgtagcccga cggggaattc cttctgcgtg agtgtgcggg aataattaaa aacgatgcac
     7741 accgggtttt taccgcgtta atgattcgcg ggtttatccc ggtgcgatgg tggaagaaac
     7801 aggaagctgt attacagaaa gtgctactac tgtatcccga tgcgatgtat gtaatgtgag
     7861 tcagataatg gcacaggatg tggtgatgtg gcagtctgga acacaggata tattgtcaga
     7921 ataagacccg taggaataaa aatgaaaaga cgccttttac tactttttct gttatctgtc
     7981 ctggcagtgg gatgctcgca gcaaaaagct gatgagcccc ggcaattagt gacggtgtat
     8041 ccacgatatc cggaatatgc tgcagcaaat tatatcaagg ggctggttga ggttaagttc
     8101 gatattggtg ctgatgggac tgtgacacgg atcgtttttc tccgctcaga gcctcataat
     8161 ttgtttcgtg atgaagtggt gaaggccatg gcgaaatggc gatttgaaaa gaatcgcccc
     8221 tgtcagggag tgaagagaca atttatcttt acgccgtcac gtccttgatg cttccaggta
     8281 gagaggggct ggaagcagga gaaaaatgaa agagccagcg gttatatttt tgtcatggct
     8341 gacgaggaat gatggaagaa ggcgttgtat gccacacaac gcctcactgt tcatttcttc
     8401 tttttctctg gtggaacccg atgaataaga gttgcactgg tttccgatga gatggcgata
     8461 tactcgggca aagtatgctg gcagttttcc aactggtcaa aaatacctgc tctcgtctgt
     8521 tgcaatgcct gcagcatgcg gcggcaatgc gccttgcttt tactaaccat ctttccttcc
     8581 tctatcagtc gctgcgtgaa ctcatcatgg aataccaggt aaatgcggat gttatcggtt
     8641 ttggctacgc agcatagtac aaaacggaca ggtgcatccc gggacggggg aggcgtcaca
     8701 tgtccctgtg atggttgttc cgggtaatgt actgtgtggg gcataaaaat gtccgataat
     8761 tttactttct accgcagtta gttgattcgt tggtcctggt agcacattgg gcgaggattt
     8821 aaatgccagg caactgaagg atgatgtcgc aagggagata gcgagaatat ttctgatttt
     8881 catttgatga tgcctctgtg tgaaatgacg gtaaacgacg cacttgtgcc ggcacataat
     8941 agcaagcacc ataatagatc agattcgatt cttgctgtaa gtgataatta ttctcgtttt
     9001 cgggtccttt ccgtcgatcc aacaggttac ggggcggcga cctcgcgggt tttcgctatt
     9061 tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gccgtaactt aatgttttta
     9121 tttaaaacac tccctgaaaa gaaaggaaac gacaggtgct gaaaacgggc tttttggcct
     9181 ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtca acaaaaagca
     9241 gctggctgac attttcggcg cgagtatccg tactattcag aactggcagg agcagggaat
     9301 gcccgttctg cgaggcggtg ggaagggtaa tgaggtgctt tatgactctg ccgacgtcat
     9361 aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaggttga
     9421 agaactgcga caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca
     9481 tcgacttacg cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga
     9541 agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag
     9601 tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca
     9661 tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg cgctggatga
     9721 actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg ctgcggcatt
     9781 ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acagaccgcc gttgaatggg
     9841 cggatgctaa ttactatctc ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac
     9901 tgccctttca gcgggccatc atgaatgcga tgggcagcga ctacatccgt gaggtgaatg
     9961 tggtgaagtc tgcccgtgtc ggttattcca aaatgctgct gggtgtttat gcctacttta
    10021 tagagcacaa gcagcgcaac acccttatct ggttgccgac ggatggtgat gccgagaact
    10081 ttatgaaaac ccacgttgag ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc
    10141 cgtggtatgg caaaaagcac cgggataaca cgctcactat gaagcgtttt tccaatggtc
    10201 gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg
    10261 tggcgggtta tgatgaactt gctgcctttg atgaggatat tgaacaggaa ggctctccga
    10321 cgttccttgg cgacaaacgt attgaaggct cggtctggcc aaagtccatc cgtggctcca
    10381 cgcccaaagt gagaggcacc tgtcagattg agcgtgcagc cagtgaatcc ccgcattgta
    10441 tgcgttttca tgttgcctgc ccgcactgcg gggaggagca gtaccttaaa tttggcgata
    10501 aagagacgcc gtttggcctc aaatggacgc cggatgatcc ctccagcgtg ttttatctct
    10561 gcgaacataa tgcctgcgtc atccgccagc aggagctgga cttcactgat gcccgttata
    10621 tctgcgaaaa gaccgggatc tggacccgtg atggcattct ctggttttcg tcatccggtg
    10681 aagagattga gccgccggac agcgtgacct ttcacatctg gacggcgtac agcccgttca
    10741 ccacctgggt gcagattgtc aaagactgga tgaaaacgaa aggagatacg ggaaaacgta
    10801 aaaccttcgt gaacaccacg ctcggtgaga catgggaagc gaaaatcggt gaacgtccgg
    10861 atgctgaagt gatggcagag cggaaagagc attattcagc gcccgttcct gaccgtgtgg
    10921 cttacctgac tgccggtatc gactcccagc tggatcgcta cgaaatgcgc gtatggggat
    10981 gggggccggg tgaggaaagc tggctgattg accggcagat tattatgggc cgccacgacg
    11041 atgaacagac gctgctgcat gtggatgagg ccatcaataa aacctatacc cgccggaatg
    11101 gtgcagaaat gtcggtatcc cgtatctgct gggatattgg cgggattgac ccgaccatcg
    11161 tgtatgaacg ctcgaaaaaa catgggctgt tccgggtgat ccccattaaa ggggcatccg
    11221 tctacggaaa gccggtggcc agcatgccac gtaagcgaaa caaaaacggg gtttacctta
    11281 cggaaatcgg tacggatacc gcgaaagagc agatttataa ccgcttcaca ctgacgccgg
    11341 aaggggatga accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc
    11401 tgaccgaagc gcagcagctg actgctgaag agcaggtcga aaaatgggtg gatggcagga
    11461 aaaaaatact gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc ttcgtttatg
    11521 cgctggcggc gctgcgcatc agtatttccc gctggcagct ggatctcagt gcactgctgg
    11581 cgagcctgca ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgccc
    11641 gtgccttatc cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc
    11701 actgcatgac ctgatgacag gtaaacgggt ggcaacggta cagaaagacg gacggcgagt
    11761 ggagtttacg gccacttccg tgtctgacct gaaaaaatac attgcggagc tggaggtgca
    11821 gaccggcatg acacagcgac gcaggggacc tgcaggattt tatgtatgaa aacgcccacc
    11881 attcccaccc ttctgggacc ggacggcatg acatcgctgc gtgaatatgc cggttatcac
    11941 ggcggtggca gcggatttgg tgggcagttg cgggcgtgga acccaccggg tgaaagtgtg
    12001 gatgcagccc tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtacgc
    12061 aataacggct atgctgccaa cgccatccag ctgcatcagg atcatatcgt cgggtctttt
    12121 ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga agacgcccgt
    12181 gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc
    12241 attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac
    12301 gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc
    12361 cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac
    12421 agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac
    12481 gtcagtgagg acggctatcc tggctggatg ccgcagaaat ggacatggat accccgtgag
    12541 ttaccgggcg gtcgtgcttc gttcattcac gtctttgaac ccgtggagga cgggcagacc
    12601 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
    12661 aacacgcagc tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat cgagagtgag
    12721 ctggatacgc agtcagcgat ggattttatt cttggcgcga acagtaagga gcagcgggac
    12781 aggctgaccg gctggattgg tgaaattgcc gcgtattacg ccgcagcacc ggtccggctg
    12841 ggaggcgcaa aagtgcctca cctgatgccg ggtgactcac tgaacctgca gacggctcag
    12901 gacacggata acggctactc cgtgtttgag cagtcactgt tgcggtatat cgctgccggg
    12961 ctgggtgtct cgtatgagca gctttcccgg aattacgccc agatgagcta ctccacggca
    13021 cgggccagtg cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc
    13081 cgtcaggcga gccagatgtt tctgtgctgg ctggaagagg ccatcgctcg ccgcgtggtg
    13141 acgttacctt caaaagcgcg cttcagtttt caggaagccc gaagtgcctg ggggaactgc
    13201 gactggatag gctccggtcg tatggccatc gatggtctga aagaagtaca ggaagcggtg
    13261 atgctgatag aagccggact gagtacctac gagaaagagt gcgcaaaacg cggtgacgac
    13321 tatcaggaaa tttttgccca gcaggtccgt gaaacgatgg agcgccgtgc agccggtctt
    13381 aaaccgcccg cctgggcggc tgcggcattt gaatccgggc tgcgacaatc aacagaggag
    13441 gagaagagtg acagcagagc tgcgtaatct cccgcatatt gccagtatgg cctttaatga
    13501 gccgctgatg cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg caggccagct
    13561 tgggatcagc cgcctgacgg atgcggtatc cggcgacagc ctgactgccg gagaggcacc
    13621 cgcggcgctg gcgttatccg ttgatgatga cggaccacga caggcccgca gttatcaggt
    13681 catgaacggc atcgccgtgc tgccggtgtc cggcacgctg gtcagtcgga cgcgagcgct
    13741 gcagccgtat tcggggatga ccggttacaa cggcattatc gcccgtctgc aacaggctgc
    13801 cagcgacccg atggtggacg gcattctgct cgatatggat acgccaggcg ggatggtggc
    13861 aggggcattt gactgcgctg acatcatcgc ccgtgtgcgt gacataaagc cggtatgggc
    13921 gctggccaac gacatgaact gcagtgcagg tcagttgctt gccagcgccg cctcccgacg
    13981 tctggtcacg cagaccgccc ggacaggctc catcggcgtc atgatggctc acagtaatta
    14041 cggtgctgcg ctggagaaac agggcgtgga aatcacgctg atttacagcg gcagccataa
    14101 ggtggatggc aacccctaca gccatctacc ggatgatgtc cgggaaacac tgcagtcccg
    14161 gatggatgca acccgccgga tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt
    14221 gcaggctgtg ctggataccg aggctgcagt gtacagcggt caggaggcca ttgatgccgg
    14281 actggctgat gaacttgtta acagtaccga tgcgatcacc gtcatgcgtg atgcactgga
    14341 tgcacgtaaa tcccgtctct caggagggcg aatgaccaaa gagactcaat caacaactgt
    14401 ttcagccact gcttcgcagg ctgacgttac tggcgtggtg ccagcgacgg agggcgagaa
    14461 cgccagcgct gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa
    14521 cagtcgcatt atggggatcc tcaactgtga ggaagctcac ggacgcgaag aacaggcacg
    14581 cgtgctggcc gaaacccctg gtatgaccgt ggaaacggcc cgccgcattc tggcagttgc
    14641 accacagagt gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc
    14701 ggcaccgctg gctgcaggta acctggcatc tgatgccgtt aacgatttgc tgaacacacc
    14761 agtgtaaggg atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa
    14821 cagtgacccg gctcataccg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat
    14881 gaccccgctg atgctggaca cctccagccg taagctggtt gcgtgggatg gcaccaccga
    14941 cggtgctgcc gttggcattc ttgcggttgc tgctgaccag accagcacca cgctgacgtt
    15001 ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga
    15061 gacgaaaaaa cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac tttacccttc
    15121 atcactaaag gccgcctgtg cggctttttt tacgggattt ttttatgtcg atgtacacaa
    15181 ccgcccagct gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc
    15241 tctttttccg tgagagctat cccttcacca cggagaaagt ctatctctca caaattccgg
    15301 gactggtaaa catggcgctg tacgtttcgc cgattgtttc cggtgaggtt atccgttccc
    15361 gtggcggctc cacctctgaa tttacgccgg gatatgtcaa gccgaagcat gaagtgaatc
    15421 cgcagatgac cctgcgtcgc ctgccggatg aagatccgca gaatctggtg gacccggctt
    15481 accgccgccg tcgcatcatc atgcagaaca tgcgtgacga agagctggcc attgctcagg
    15541 tcgaagagat gcaggcagtt tctgccgtgc ttaagggcaa atacaccatg accggtgaag
    15601 ccttcgatcc ggttgaggtg gatatgggcc gcagtgagga gaataacatc acgcagtccg
    15661 gcggcacgga gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag
    15721 cctacgcgct gaacgccagc ggtgtggtga atatcatcgt gttcgatccg aaaggctggg
    15781 cgctgttccg ttccttcaaa gccgtcaagg agaagctgga tacccgtcgt ggttctaatt
    15841 ccgagctgga gacagcggtg aaagacctgg gtaaagcggt gtcctataag gggatgtatg
    15901 gcgatgtggc catcgtcgtg tattccggac agtacgtgga aaacggcgtc aaaaagaact
    15961 tcctgccgga caacacgatg gtgctgggga acactcaggc acgcggtctg cgcacctatg
    16021 gctgcattca ggatgcggac gcacagctcg aaggcattaa cgcctctgcc cgttacccga
    16081 aaaactgggt gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc
    16141 tgatgctgct ggctgaccct gatgagttcg tgtccgtaca actggcgtaa tcatggccct
    16201 tcggggccat tgtttctctg tggaggagtc catgacgaaa gatgaactga ttgcccgtct
    16261 ccgctcgctg ggtgaacaac tgaaccgtga tgtcagcctg acggggacga aagaagaact
    16321 ggcgctccgt gtggcagagc tggaagagga gcttgatgac acggatgaaa ctgccggtca
    16381 ggacacccct ctcagccggg aaaatgtgct gaccggacat gaaaatgagg tgggatcagc
    16441 gcagccggat accgtgattc tggatacgtc tgaactggtc acggtcgtgg cactggtgac
    16501 gctgcatact gatgcacttc acgccacgcg ggatgaacct gtggcatttg tgcttccggg
    16561 aacggcgttc cgtgtctctg ccggtgtggc agccgaaatg acagaacgtg gcctggccag
    16621 aatgcaataa cgggaggcgc tgtggctgat ttcgataacc tgttcgatgc tgccattgtc
    16681 cgcgccgatg aaacgatacg cgggtacatg ggaacgtcag ccaccatgac atccggtgag
    16741 cagtccggcg cagtaatacg tggtgttttt gatgaccctg aaaatatcag ctatgccgga
    16801 cagggcgtgc gcgttgaagg ctccagcccg tccctgtttg tccggactga tgatgtgcgg
    16861 cagctgcggc gcggcgacac gctgaccatc ggtgaggaaa acttctggat agaccggatt
    16921 tcgacggatg atggcggaag ctgtcatctc tggcttgggc gtggcgtgcc gcctgccgtt
    16981 aaccgtcgtc gctgaagggg ggatgtatgg ccataaaagg tcttgagcag gccgttgaaa
    17041 acctcagccg tatcagcaga acggcggtgc ccggtgccgc cgcaatggcc attaaccgcg
    17101 ttgcttcatc cgcgatatcg cagtcggcgt cacaggttgc ccgtgagaca aaggtacgcc
    17161 ggaaactggt aaaggaaagg gccaggctga aaagggccac ggtcaaaaat ccgcaggcca
    17221 gaatcaaggt taaccggggg gatttgcccg taatcaggct gggtaacgcg cgggttgtcc
    17281 tgtcccgacg caggcgtcgt aaaaaggggc agcgttcatc cctgaaaggt ggcgacagcg
    17341 tgcttgtggt gggaaaccgt cgtattcccg gcgcgtttat tcagcaactg aaaaatggcc
    17401 gatggcatgt catgcagcgt gtggccggga aaaaccgtta ccccattgat gtggtgaaaa
    17461 tcccgatggc agtgccgctg accacggcgt ttaaacagaa tattgagcgg atacggcgtg
    17521 aacgtcttcc gaaagagctg ggctatgcgc tgcagcatca actgaggatg gtaataaagc
    17581 gatgaaacat acagaactcc gtgcagccgt actggatgca ctggaaaaac atgacaccgg
    17641 ggcgacgctt tttgatggtc gccccgctgt ttttgatgag gaggattttc cggcagttgc
    17701 cgtttatctc accggcgctg aatacacggg cgaagagctg gacagcgata cctggcaggc
    17761 ggagctgcat attgaagttt tcctgcctgc tcaggtgccg gattcagagc tggattcgtg
    17821 gatggagtcc cggatttatc cggtgatgag cgatgtcccg gcactgtcag atttgatcac
    17881 cagtatggtg gccagtggct atgactaccg gcgcgacgat gatgcgggcc tgtggagttc
    17941 agccgatctg acttatgtca ttacctatga aatgtgagga cgatatgcct gtaccaaatc
    18001 cagtaatgcc ggtgaaaggg gccgggacca ccctgtgggt ttataagggg aacggtgacc
    18061 cttatgcgaa cccgctttca gacgttgact ggtcgcgtct ggctaaagtt aaagacctga
    18121 cgcccggcga actgagcgct gagtcctatg acgacagcta tctcgatgat gaagatgcgg
    18181 actggactgc gaccgggcag gggcagaaat ctgccggaga taccagcttc acgctggcgt
    18241 ggatgcccgg agagcagggg cagcaggcgc tgctggcgtg gtttaatgaa ggggataccc
    18301 gtgcctataa aatccgcttc ccgaacggca cggtcgatgt gttccgcggc tgggtcagca
    18361 gtatcggtaa ggcggtgacg gcgaaggaag tgatcacccg cacggtgaaa gtcaccaacg
    18421 tgggacgtcc gtcgatggca gaagatcgca gcacggtgac ggcgacaacc ggcatgacgg
    18481 tgacacccgc cagtgcttcc gtagtgaaag ggcagagcac tacgctgacc gtggcattcc
    18541 agccggaggg cgcaaccgac aagagcttcc gtgcggtgtc tgcggataaa acaaaagcca
    18601 ccgtgtcggt cagcggtatg accatcaccg tgaacggcgt tgctgcaggc aaggtcaaca
    18661 ttccggttgt atccggtaat ggtgagtttg ctgcggttgc agaaatcacc gtcaccgcca
    18721 gttaatccgg agagtcagcg atgttcctga aaaccgaatc atttgaatat aacggcgtga
    18781 ccgtcacgct ttctgaactg tcagccctgc agcgtattga gcatcttgcc ctgatgaaac
    18841 ggcaggcaga accggcggga tccgacagca accggcaggt tactgtggaa gacgtcatca
    18901 gaaccggcgc gtttctggtg gcgatgtccc tgtggcataa ccatccgaag aagacgcaga
    18961 tgccgtccat gaatgaagcc gttaaacaga ttgagcagga agtgcttacc acctggccca
    19021 cagaggcaat ttctcatgct gaaaacgtgg tgtaccggct gtccggtatg tatgagtttg
    19081 tggtgaataa tgcccccgaa caggcagagg acgccgggcc tgcagagcct gtttctgcgg
    19141 gaaagtgttc gacggtgagc tgagttttgc cctgaaactg gcgcgtgaga tggggcgacc
    19201 cgactggcgt gccatgcttg ccgggatgtc atccacggag tatgccgact ggcaccgctt
    19261 ttacagtacc cattattttc atgatgttct gctggatatg cacttttccg ggctgacgta
    19321 caccgtgctc agcctgtttt tcagcgatcc ggatatgcat ccgctggatt tcagtctgct
    19381 gaaccggcgc gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg
    19441 gcttgccgga ggtgtccgct ttggcccgga cgggaatgaa gttatccccg cttccccgga
    19501 tgtggcggac atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg
    19561 aggagtccgg tatggctgaa ccggtaggcg atctggtcgt tgatttgagt ctggatgcgg
    19621 ccagatttga cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaagtgatg
    19681 cgaaaaaaac agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga
    19741 aagcggggat ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca
    19801 ccgacgtggc cacgcagctt gcaggcgggc aaagtccgtg gctgatcctg ctgcaacagg
    19861 gggggcaggt gaaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggtg
    19921 cgatcaccct gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt
    19981 atgcctggta tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg
    20041 gcaatcaggc gggtctgacg gcagatcgta tgctggtcct gtccagagcc gggcaggcgg
    20101 cagggctgac gtttaaccag accagcgagt cactcagcgc actggttaag gcgggggtaa
    20161 gcggtgaggc tcagattgcg tccatcagcc agagtgtggc gcgtttctcc tctgcatccg
    20221 gcgtggaggt ggacaaggtc gctgaagcct tcgggaagct gaccacagac ccgacgtcgg
    20281 ggctgacggc gatggcacgc cagttccata acgtgacggc ggagcagatt gcgtatgttg
    20341 ctcagttgca gcgttccggc gatgaagccg gggcattgca ggcggcgaac gaggccgcaa
    20401 cgaaagggtt tgatgaccag acccgccgcc tgaaagagaa catgggcacg ctggagacct
    20461 gggcagacag gactgcgcgg gcattcaaat ccatgtggga tgcggtgctg gatattggtc
    20521 gtcctgatac cgcgcaggag atgctgatta aggcagaggc tgcgtttaag aaagcagacg
    20581 acatctggaa tctgcgcaag gatgattatt ttgttaacga tgaagcgcgg gcgcgttact
    20641 gggatgatcg tgaaaaggcc cgtcttgcgc ttgaagccgc ccgaaagaag gctgaacagc
    20701 agagtcaaca ggacaaaaat gcgcagcagc agagcgatac cgaagcgtca cggctgaaat
    20761 ataccgaaga ggcgcagaag gcttacgaac ggctgcagac gccgctggag aaatatgccg
    20821 cccgtcagga agaactgaat aaggccctga aagacggaaa aatcctgcag gcagattaca
    20881 acacgctgat ggcggcagcg aaaaaggact atgaaacgac actgaaaaag ccgaagcagt
    20941 ccggcgtgaa ggtgtctgcg ggcgatcgtc aggaagacag tgctcatgct gccctgctga
    21001 cgcttcaggc tgaactccgg acgctggaga agcatgccgg agcaaatgag aaaatcagcc
    21061 agcagcgccg ggatttgtgg aaggcggaga gtcagttcgc ggtactggag gaggcggcgc
    21121 aacgtcgcca gctgtctgta caggagaaat ccctgctggc gcataaagat gagacgctgg
    21181 agtacaaacg ccagctggct gcacttggcg acaaggttac gtatcaggag cgcctgaacg
    21241 cgctggcgca gcaggcggat aaattcgcac agcagcaacg ggcaaaacgg gccgccattg
    21301 atgcgaaaag ccgggggctg actgaccggc aggcagaacg ggaagccacg gaacagcgcc
    21361 tgaaggaaca gtatggcgat aatccgctgg cgctgaataa cgtcatgtca gagcagaaaa
    21421 agacctgggc ggctgaagac cagcttcgcg ggaactggat ggcaggcctg aagtccggct
    21481 ggagtgagtg ggaagagagc gccacggaca gtatgtcgca ggtaaaaagt gcagccacgc
    21541 agacctttga tggtattgca cagaatatgg cggcgatgct gaccggcagt gagcagaact
    21601 ggcgcagctt cacccgttcc gtgctgtcca tgatgacaga aattctgctt aagcaggcaa
    21661 tggtggggat tgtcgggagt atcggcagcg ccattggcgg ggctgttggt ggcggcgcat
    21721 ccgcgtcagg cggtacagcc attcaggccg ctgcggcgaa attccatttt gcaaccggag
    21781 gatttacggg aaccggcggc aaatatgagc cagcggggat tgttcaccgt ggtgagtttg
    21841 tcttcacgaa ggaggcaacc agccggattg gcgtggggaa tctttaccgg ctgatgcgcg
    21901 gctatgccac cggcggttat gtcggtacac cgggcagcat ggcagacagc cggtcgcagg
    21961 cgtccgggac gtttgagcag aataaccatg tggtgattaa caacgacggc acgaacgggc
    22021 agataggtcc ggctgctctg aaggcggtgt atgacatggc ccgcaagggt gcccgtgatg
    22081 aaattcagac acagatgcgt gatggtggcc tgttctccgg aggtggacga tgaagacctt
    22141 ccgctggaaa gtgaaacccg gtatggatgt ggcttcggtc ccttctgtaa gaaaggtgcg
    22201 ctttggtgat ggctattctc agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta
    22261 cagcgtgacg ctttctgtcc cccgtgagga ggccacggta ctggagtcgt ttctggaaga
    22321 gcacgggggc tggaaatcct ttctgtggac gccgccttat gagtggcagc agataaaggt
    22381 gacctgcgca aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca gcgcagagtt
    22441 tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt
    22501 gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc tgacagaggt cggtggagaa
    22561 cgttattttt tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcga
    22621 cagtatcagc cgtatcccat tcaggggagc ggttttgaac tgaatggcaa aggcaccagt
    22681 acgcgcccca cgctgacggt ttctaacctg tacggtatgg tcaccgggat ggcggaagat
    22741 ctgcagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat
    22801 gcggtgaact tcgtcaacgg aaacagttat gccgatccgg agcaggaggt gatcagccgc
    22861 tggcgcattg agcagtgcag cgaactgagc gcggtgagtg cctcctttgt actgtccacg
    22921 ccgacggaaa cggatggcgc tgtttttccg ggacgtatca tgctggccaa cacctgcacc
    22981 tggacctatc gcggtgacga gtgcggttat agcggtccgg ctgtcgcgga tgaatatgac
    23041 cagccaacgt ccgatatcac gaaggataaa tgcagcaaat gcctgagcgg ttgtaagttc
    23101 cgcaataacg tcggcaactt tggcggcttc ctttccatta acaaactttc gcagtaatcc
    23161 catgacacag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc
    23221 gtgtggcttc gtggtgagaa cgccgaaggg ggaaagatat tttccctgcg tgaatatctc
    23281 cggtgagccg gaggcgtatt tccggatgtc gccggaggac tggctgcggg cagagatgca
    23341 gggtgagatt gtggcgctgg tccacagcca ccccggtggt ctgccctggc tgagtgaggc
    23401 tgacaggcgg ctgcaggtgc agagcgattt gccgtggtgg ctggtctgcc ggggtgagat
    23461 tcataaattc cgctgtgtgc cgcatcttac cgggcggtgc tttgagcacg gggtgacgga
    23521 ctgttacacg ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca
    23581 tcgcggggat gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac
    23641 agggctgtat caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt
    23701 tggttcatcg gtgccgaatc atgccgccat ttactgtggt gatggcgagc tgctgcacca
    23761 tattcctgaa caactgagca aacgagagag gtataccgac aaatggcagc gacgcacaca
    23821 ctccctctgg cgtcaccggg catggcgcgc atctgccttt acggggattt acaacgattt
    23881 ggccgccgca tcgaccttcg tgtgaaaacg ggggctgaag ccatccgggc gctggccaca
    23941 cagctcccgg cgtttcgtca gaaactgagc gacggctggt atcaggtacg gattgccggg
    24001 cgtgatacag gtgaaacgga attatcagcc cgtcttaatg aaccgctggc aaatggtgct
    24061 gtgatccata tcgtgccgcg tctggcagga gccaaaagtg gcggtgtgtt tcaggctgtg
    24121 ctgggggcgg ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt
    24181 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggcc
    24241 cctaaaccca gaaccccccg cacacagaca acggataacg gcaaacagaa cacctatttt
    24301 tcttcactgg ataatatggt tgcccagggc aatgttctgc ccgttctgta cggtgaaatg
    24361 cgcgtgggat cacgtgtggt atctcaggag atcagcacag cagatgaagg tgatggtggt
    24421 caggttgtgg tgattggtcg ctgatgaaaa acgttttatg tgaaaccgcc tccgggcggt
    24481 tttatcgttt atggagcatg acgaatgggt aaaggcagca gtaaggggca taccccgcgc
    24541 gaagcgaagg acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc catcagcgaa
    24601 gggccggttg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccggtg
    24661 ctggacagtg aggggaatac caatatatcc ggcgtcacgg tggtgttccg ggccggtgag
    24721 caggagcagt caccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg
    24781 gaagtgaaat atgacacgcc gatcacccgg accatcacgt cggcaaacat cgaccgtctg
    24841 cgctttacct tcggtgtaca ggcactggtg gaaaccacct caaagggaga caggaatccg
    24901 tcggaagttc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
    24961 atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt ggataacctg
    25021 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc cggacagcac cacagaccag
    25081 ctgctgaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
    25141 ccgaacacgg cactggttgg cgtgcaggtg gactcggagc agttcggcag ccagcaggtg
    25201 agccgtaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
    25261 acgcggcaat acagcggtat ctgggacgga acgttaaaac cggcatacag caacaacatg
    25321 gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa acgtcttggt
    25381 gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcggtg
    25441 ccggacggct ttggcggcac ggagccgcgc attacctgta atgcgtacct gaccacacag
    25501 cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
    25561 aacgggcaga cgctgacgtt cgtgcaggac cgaccatcag ataaggtgtg gacctataac
    25621 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
    25681 aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cggataacgg ctgggagacg
    25741 gcgacagagc tcgtggagga tacgcaggcc attgcccgtt acggccgtaa cgtcacgaag
    25801 atggatgcct ttggctgtac cagccggggg caggcacacc gcgccgggct gtggctgatt
    25861 aaaacggagc tgctggaaac gcagaccgtg gacttcagcg tgggtgctga agggcttcgc
    25921 catgtaccgg gggatgtcat tgaaatctgc gatgatgact atgccggtat cagcaccggt
    25981 ggtcgtgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
    26041 ctgccatcct ccggtaccac gctgataagc ctggttgacg gaagtggcaa tccggtcagc
    26101 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgtgt tcctgacggt
    26161 gttgctgaat acagcgtatg ggggctgaag ctgccgacgc tgcgccagcg actgttccgc
    26221 tgcgtgagta tccgtgaaaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
    26281 ccggaaaaag agaccatcgt ggataacggg gcgcactttg acggcgaaca gagtggcacg
    26341 gtgaatggtg tcacgccgcc cgcggtgcag cacctgaccg cagaagtcac cgcagacagc
    26401 ggggaatatc aggtgctggc gcgatgggac acaccgaagg tggtgaaggg ggtgagcttt
    26461 atgcttcgcc tgaccgtggc agcggatgac ggcagtgagc ggctggtcag cacggccagg
    26521 acgacggaaa ccacataccg cttcaggcaa ctggcgctgg ggaactacag gctgacagtc
    26581 cgggcggtaa atgcgtgggg gcagcagggg gatccggcgt cggtatcgtt ccggattgcc
    26641 gcaccggcag cgccatcgcg gattgagctg acgccgggct attttcagat aaccgcaacg
    26701 ccgcatcttg ccgtttatga tccgacggta cagtttgagt tctggttctc ggaaaagcgg
    26761 attgcggata tcaggcaggt tgaagccagc gcgcgttatc ttggtacggc gctgtactgg
    26821 atagccgcca gtatcaatat caaaccgggc catgattatt atttttacat tcgcagtgtg
    26881 aacaccgttg gcaaatcggc atttgtggag gctgttggcc agccgagtga tgatgcatcc
    26941 ggctatctgg attttttcaa aggagagata gggaaaaccc atctggctca ggagttgtgg
    27001 acgcagattg ataacggtca gcttgcgcct gacctggctg aaatcaggac gtccattacg
    27061 gatgtcagca atgaaatcac gcagaccgtc aataagaaac tggaagacca gagtgcagcg
    27121 atccagcaga tacagaaggt tcaggttgat acaaataata acctgaacag catgtgggct
    27181 gtgaagctgc agcagatgca ggacggacgc ctttatatcg cgggtattgg tgccggtatt
    27241 gagaataccc ctgacggtat gcagagtcag gtgctgctgg cggcggacag gattgcgatg
    27301 attaatcctg cgaatggcaa tacaaagccg atgtttgttg ggcagggcga tcagatattc
    27361 atgaacgaag tgttcctgaa atatctgacg gctcccacca ttaccagcgg tggcaatccc
    27421 ccaacgtttt cactgacacc tgatggtcga ctttctgcga gaaatgcgga tatcagcggt
    27481 aacgtgaatg cgaactccgg gacgctcaat aatgtcacga ttaaccagaa ctgtcggatt
    27541 ctgggaaaac tgtcagctaa ccagattgaa ggtgatattg tcaaaacggt gggaaaagcc
    27601 tttccgagaa atggcagtta tgccagcggt acaataacgg tcactgtgta cgatgaccag
    27661 gcttttgacc gtcagatagt cgtccccccc gttctgtttc gcggtggtaa gcatgaaaac
    27721 ttcaacagca acaaccaaca gtcatactgg tattcaacct gtaagctgca ggtgctgaag
    27781 aacggacagg aaatctttca gcaacccgcg acggatgtca gcagggtatt ttcatcagtc
    27841 attgatatgc ctgccggaca cggccatgtc accctgactt tcaatgtttc ttcatatggt
    27901 gcaaataact ggacgccaac gaccagtatc agcgaccttc ttgttgtcgt gatgaagaaa
    27961 tcaacagccg gcatcagtat cagttgaatt ttaaaaccca aattcgggca ccagaaatgg
    28021 tgcctttttt atttgtggag tgaatatggc agtacagatt tcaggtgtgc tgaaagacgg
    28081 tgcaggaaaa ccgatacaga actgcaccat tcagctcaaa gcaaaacgta acagcaccac
    28141 agttgtggtg aacacggtgg cctcagaaaa tccggatgaa gccggacgtt acagcatgga
    28201 tgttgagtat ggccagtaca gcgtcgccct gctggttgaa ggttttccgc cttcacatgc
    28261 cgggaccatc accgtgtatg aggattctaa gccggggaca ttgaatgatt ttctgggcgc
    28321 tgcaacagaa gatgatgttt gtccggaggc actgtatcgt tttgaaaaga tggtggaaga
    28381 ggcggcacgc aacgctgaag ccgcctctca gagcgcagcg gcagcaaaga aatcagaaac
    28441 agcagcggca tcgtccagga acgcggcgaa aacatcagag acgaatgcag gtaacagcgc
    28501 gaaagcggca gcttcttcaa aaacagccgc acaaaacgca gcaacagcgg cagaacgttc
    28561 agagacaaat gcccgtgcgt cagaagaagc ctccgcagac agtgaagagg cttcccgccg
    28621 taatgcagag tcagccgctg aaaatgccgg agtcgccacc acaaaagcgc gggaggccgc
    28681 agcagacgca acaaaggccg ggcagaaaaa ggatgaggct ctgtcggcag cgacacgagc
    28741 tgaaaaggcg gcagaccgcg cagaagccgc agcggaagtg actgcagagc cctgtgcgaa
    28801 tatagtgccg ccgctgcctg atgtgtggat accgtttaac gattcactgg atatgattac
    28861 gggtttttcg ccatcttata aaaagatagt tattggtgac gatgaaataa caatgccagg
    28921 cgacaagatt gttaagttta aacgtgcttc aacagcaacg tatattaata agtccggcca
    28981 actcaagctt gctgaagttg acgaaccgcg atttgagcgc gatggcttat tgattgaagg
    29041 acagaggaca aattatctga ggaactcaaa taaaccagac tcatggactg ttcattccgc
    29101 actgaataaa acatttggca ctgataaaca ggggttcaat tatgccacgg tgacacccac
    29161 ggaaagtata gtgggaacaa caggtggcta tactgtgcat ggtgtggttg cagcagacag
    29221 attcccgctg gcaagtggtg aatgtttcac tttttcgtgc cgggttaaag gcgctaaagc
    29281 acgatgcagg ttaagagttt cagttattat tggtggaaca gataccttct ctgctgactc
    29341 ttatcttgat ctggataccc ggatcgcaac agtaagcggt aatacatccc ttataacagc
    29401 caaagctgaa caacagggcg agtggaccta ctatgaggcc acttatacag ctaatacgga
    29461 cattgatacc gttaactgtg ctttttatat gacaaataaa ataagtaatg agacattcta
    29521 tgatgactca acattaacca tgacgacgcc gcaaattgaa ctgggcaata cggcatcgtc
    29581 atttattgta actacaatgc caacaacacg cgcaagtgat gtagttacta tcccatcgca
    29641 gaataacctg tcaacacggc cttttacggt attgtgcgaa gtaagcagga actggagtac
    29701 accgcccaat gttgcgccaa ggatatttga tgttggaggg cacagtattg atgataatta
    29761 tttatcgctg gggtttgttg caacaggaaa gataagcgcc aacgtaggaa tggttcagcc
    29821 acaaattgcc tcagatggag aaaggttcat tgtgggtgtg agagctaaat ctgatttatc
    29881 agtaaatgca atatgcaatg gtaattatac aacaaacctt aatggtaaaa tatttggagt
    29941 tacagcaaca tcgtaccggt ttggtgggca gaccgcagca ggaacgcgtc atttgtttgg
    30001 acacatcaga aatttcagag tctggtttaa agaattaaat gacaggcaaa tcaaggaggc
    30061 agtatgaaag atttaacttt gaaatttcct ggtaacagag agtttaaatc cttcctgtca
    30121 tctcttgact gggaggagga tgaagacctc cagaataaac tgttagtcga tgaaattggt
    30181 ttcacctaca cagaaacagg ggtaacagaa gagggagaac ctgtctgtgt ccggaatgac
    30241 ggttattttg tcaacattcg cattcttgat gacttgtttg atgtttctgt attctctgat
    30301 tatgtcgtgg agctggaaac accgcttcgg gaatggagct gaaaggagga aataatggat
    30361 ataagcccct tacttcatgc actttgtgct gtggctgcgc agatactggt tggtcttttt
    30421 accggaaact gggcttacgg agcgatagcc ggttgtacgt tcttcattgc gcgtgaacat
    30481 acccaggcag aatatcgctg gattgaaatg ttcgggcatg gcaagcgaat gaatatgccg
    30541 tggtggggcg gttttgatcc gcgagcttgg gatgtggcaa gcctgatgga ttttgctgtg
    30601 ccggtggtgg cgtgtctgct ggtctggctg ttggttaatc gtgggtgaaa aaaggtgagc
    30661 tgtatatgca acggaggaag aaaccccgtt gctggaagcc aggaaaaagt atcgggtgtt
    30721 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gagtttccct
    30781 ttttgcgtgc gttgatgctg gtaacggcaa acgtcatgat gaacaaaacc gcgaataaga
    30841 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga caaccagaca ccagtcattg
    30901 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
    30961 gtaccgcaat ggcgccaggc gcaatgagca gagaaatgga gagaatgata cctaccgctt
    31021 tcagcgtcgc cacaatggtc agagagacca tgcacagcag cccataatgc agccatctcg
    31081 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
    31141 attttacgct aataacaagt gtaaccagcc cggcaataat catcgtctgg attatatcgc
    31201 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttga
    31261 ctgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccatcactg
    31321 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgca cagaatactc
    31381 cggcgacgaa tgcccctgta gccaaaggta accccagaat ccaggcaagc acgatgccag
    31441 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
    31501 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
    31561 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgccttatg
    31621 cgcacgccga cgatttgcca aaagcccatg ggtgggcgca aagacaaatg ctaaaagaaa
    31681 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
    31741 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaatcatga gcaggcgggg
    31801 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
    31861 aaaggcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
    31921 gagttttaat ctgcccggat gcaatccaat agcacgggcg tgattctcgt cgaaaaaggt
    31981 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
    32041 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
    32101 gttcacagat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
    32161 gatcagaccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
    32221 gcccgcagcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
    32281 tgcccccgca acacccggga caatagagtg cgacaacgca tcgccaatca gcgaccagcc
    32341 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccatcg ccgagaccca
    32401 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tctttcttcc
    32461 ctctgcaccg ctgacggtcg atgagccaca aaagggcgtt catcgtcagt aataatgctt
    32521 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctctaggttc
    32581 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
    32641 gtataatcgc aaaatgtcgt gacggatccg agattgtgtg ttgagacaag catcgttttg
    32701 ccttccgcgc gtaactccct caacaggctg atgattttgg cttcggtttt cacatcaacg
    32761 ccagtaaaag gttcatccag caggatcaca tcaccctgct gtgcaatcgc tctcgccaga
    32821 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gtcgatggcg aaaattcacc
    32881 atatcgacac gttccagcgc atcggtgaca atttgacgat ccctcttttt ggcgatacgc
    32941 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
    33001 gaccagtcaa catcttctga ctgagggacg taggcaacca ggtttttctg taacgcctgt
    33061 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
    33121 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
    33181 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
    33241 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccagctttaa
    33301 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgtc ggtaccgggc
    33361 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
    33421 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
    33481 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
    33541 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
    33601 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
    33661 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattgcgt tggtaggttt
    33721 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
    33781 cattatctgg cgacatccat gcatgggggt taggtttgcc ctcatagggt ccttcggtga
    33841 tccctacagg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
    33901 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
    33961 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
    34021 ttatggatga gacttctgca gcatctcccg ccacgttttt ggccatatcc gcgatgatgg
    34081 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
    34141 tgagtgcgag cccccccaag agcatggtta ctttttttat cgagtgcata atttagtacc
    34201 tatagtgagc aaaattagaa caaatatagc aacggctatg ttttatagca cagcctatac
    34261 aaaatgaaaa tgattatcat tagcagcttt gaggcccaaa acagggatta ggttgtttcc
    34321 tgctggcgac gcgttattga ggaaaaaagt aaggcagcta gtaggctttg aaataacgtt
    34381 ttgcggttgg ctgatgcaat ttcgagagtg catgtattga atgatttcta gccgttactg
    34441 attttcgtat tttttgtatg ggaggatttg cacctccccc caccgagcct ccatgacttt
    34501 actccactgt ctctagggct gctgtgtgcc aggagcggaa gcaggggggc cagagactcc
    34561 ttaaaagttg atagctcgtt ttgcagggag acttacacgg taagcgttat tccgaagagc
    34621 ttaaaattga agcagtcaaa cgggttgttg agcgtgttta tgcgctgcca tctgattaat
    34681 cgacggaagc agttccgttt ggtctatgat gcagttgtcc ggcaggcgct ctgtgacgca
    34741 aaagaacgat aggagtagca ttgaagtgaa c
//
LOCUS       DAPQZD010000035        34361 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPQZD010000035 DAPQZD010000000
VERSION     DAPQZD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.035"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            7..747
                     /locus_tag="RO454_001656"
     CDS             7..747
                     /locus_tag="RO454_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDY0933503.1"
                     /translation="MFFNTKYTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     VLPKDRESVFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPL
                     ALKWSWSGSEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTG
                     LNGKANSAKVHFYAINDFGGAIEGNASL"
     gene            782..3382
                     /gene="htrE"
                     /locus_tag="RO454_001657"
     CDS             782..3382
                     /gene="htrE"
                     /locus_tag="RO454_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDY0933504.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3399..3971
                     /locus_tag="RO454_001658"
     CDS             3399..3971
                     /locus_tag="RO454_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0933505.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            3986..4588
                     /gene="yadL"
                     /locus_tag="RO454_001659"
     CDS             3986..4588
                     /gene="yadL"
                     /locus_tag="RO454_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDY0933506.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4615..5211
                     /locus_tag="RO454_001660"
     CDS             4615..5211
                     /locus_tag="RO454_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0933507.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            5263..6516
                     /locus_tag="RO454_001661"
     CDS             5263..6516
                     /locus_tag="RO454_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDY0933508.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VAVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6629..7423
                     /gene="panB"
                     /locus_tag="RO454_001662"
     CDS             6629..7423
                     /gene="panB"
                     /locus_tag="RO454_001662"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDY0933509.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7435..8286
                     /gene="panC"
                     /locus_tag="RO454_001663"
     CDS             7435..8286
                     /gene="panC"
                     /locus_tag="RO454_001663"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDY0933510.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8368..8565)
                     /locus_tag="RO454_001664"
     CDS             complement(8368..8565)
                     /locus_tag="RO454_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0933511.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLALRLLSKGM
                     SREDVAEMANLPLAEIDKMIN"
     gene            complement(8634..9554)
                     /locus_tag="RO454_001665"
     CDS             complement(8634..9554)
                     /locus_tag="RO454_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDY0933512.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEVRQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9828..10208
                     /gene="panD"
                     /locus_tag="RO454_001666"
     CDS             9828..10208
                     /gene="panD"
                     /locus_tag="RO454_001666"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDY0933513.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10212..11441)
                     /locus_tag="RO454_001667"
     CDS             complement(10212..11441)
                     /locus_tag="RO454_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDY0933514.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGLGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTQVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11505..11945)
                     /locus_tag="RO454_001668"
     CDS             complement(11505..11945)
                     /locus_tag="RO454_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDY0933515.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12050..12820)
                     /locus_tag="RO454_001669"
     CDS             complement(12050..12820)
                     /locus_tag="RO454_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0933516.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12817..13743)
                     /locus_tag="RO454_001670"
     CDS             complement(12817..13743)
                     /locus_tag="RO454_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0933517.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13852..14514
                     /gene="can"
                     /locus_tag="RO454_001671"
     CDS             13852..14514
                     /gene="can"
                     /locus_tag="RO454_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDY0933518.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14555..15091)
                     /gene="hpt"
                     /locus_tag="RO454_001672"
     CDS             complement(14555..15091)
                     /gene="hpt"
                     /locus_tag="RO454_001672"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDY0933519.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15297..17687
                     /gene="gcd"
                     /locus_tag="RO454_001673"
     CDS             15297..17687
                     /gene="gcd"
                     /locus_tag="RO454_001673"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDY0933520.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLSVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17734..19284)
                     /gene="cueO"
                     /locus_tag="RO454_001674"
     CDS             complement(17734..19284)
                     /gene="cueO"
                     /locus_tag="RO454_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDY0933521.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRNLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19450..19797
                     /locus_tag="RO454_001675"
     CDS             19450..19797
                     /locus_tag="RO454_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDY0933522.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19903..20769
                     /gene="speE"
                     /locus_tag="RO454_001676"
     CDS             19903..20769
                     /gene="speE"
                     /locus_tag="RO454_001676"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDY0933523.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20785..21579
                     /gene="speD"
                     /locus_tag="RO454_001677"
     CDS             20785..21579
                     /gene="speD"
                     /locus_tag="RO454_001677"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDY0933524.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21617..21979)
                     /locus_tag="RO454_001678"
     CDS             complement(21617..21979)
                     /locus_tag="RO454_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDY0933525.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22154..24751)
                     /gene="acnB"
                     /locus_tag="RO454_001679"
     CDS             complement(22154..24751)
                     /gene="acnB"
                     /locus_tag="RO454_001679"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDY0933526.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25106..26869
                     /locus_tag="RO454_001680"
     CDS             25106..26869
                     /locus_tag="RO454_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDY0933527.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(26940..28364)
                     /gene="lpdA"
                     /locus_tag="RO454_001681"
     CDS             complement(26940..28364)
                     /gene="lpdA"
                     /locus_tag="RO454_001681"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDY0933528.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28572..30464)
                     /gene="aceF"
                     /locus_tag="RO454_001682"
     CDS             complement(28572..30464)
                     /gene="aceF"
                     /locus_tag="RO454_001682"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDY0933529.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30479..33142)
                     /gene="aceE"
                     /locus_tag="RO454_001683"
     CDS             complement(30479..33142)
                     /gene="aceE"
                     /locus_tag="RO454_001683"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDY0933530.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33303..34067)
                     /gene="pdhR"
                     /locus_tag="RO454_001684"
     CDS             complement(33303..34067)
                     /gene="pdhR"
                     /locus_tag="RO454_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDY0933531.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8589 a         8971 c         8244 g         8557 t
ORIGIN      
        1 tggtttatgt ttttcaacac taaatataca gcagcattat gcttcgcaac ctgtgtagct
       61 tttagctcgt cagctatagc agatatcgta atttccggta cacgcgtaat ctataaaagc
      121 gaccaaaaaa gtgtcaacat tcgcctggaa aataaaggca ataacccatt acttgttcag
      181 agttggctgg atactggtga tgacaatgct gagcctggca gtattaccgt cccctttact
      241 gcgactccgc ccgtatcacg cattgatgca aaacgcggcc aaaccatcaa actgatgtac
      301 accgccagca gcgtcctgcc aaaagacaga gaaagtgtgt tctggtttaa cgtactagaa
      361 gttcctccaa aaccagatgc cgcaacagtt gcaaatcaaa gcctgctgca attggcattt
      421 cgcacacgta taaaactgtt ctatcgcccg gaagcattga aggggaatcc ctcagaagca
      481 ccattagccc tgaaatggtc ctggtcaggt tccgagggta aagcagcatt acgagtggcc
      541 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagccag cggtaaacgc
      601 tttccgattg atgtgaaaat gattgcacca ttcagtgatg aagtcatgaa agtcactggc
      661 cttaatggca aagcaaattc tgcaaaagtg catttttacg ccattaatga ttttggtggt
      721 gccattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      781 cgtgactata aaaccaacta aaaattatca caatcatctg acccgtatcg ccacgttttg
      841 cgcgctgctg tattgcaatg gtgctttctg tgctgaactt gttgaatatg accatacctt
      901 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccccgc
      961 tataccgggt gtgtacgacg ttagtgttta tgtcaacgat caacctgtta ttaaccaaag
     1021 cattacattt gtcgcaattg aaggaaaaaa gaacgcacaa gcctgtatca cacttaaaaa
     1081 tttattgcag ttccatatta attctcccga tataaacagc gaaaaagccg ttctgcttgc
     1141 ccgggatgaa gctctcggca attgcctcaa tttgacggaa attatccctc aggcttccgt
     1201 tcgttatgac gttaacgatc aacgtctgga tatagacgtt cctcaggcct gggtaatgaa
     1261 aaattaccaa aactatgttg atccatcgtt atgggaaaat ggcattaatg cagccatgtt
     1321 gtcatacaac ctcaacggat atcatagtga aacccctggt cgaaaaaatg acagcattta
     1381 tgctgctttt aacggtggga tgaatttggg tgcatggcgg ctgcgtgcct caggcaacta
     1441 caactggatg accgattctg gcggtaatta tgattttaag aatcggtata tacagcgtga
     1501 tatcgcctca ctacgctctc aattcattat tggtgagtct tatacgacgg gcgaaacctt
     1561 tgattccgtc agtatccgag gcattcgctt atacagtgac agccgcatgt tgcctccgac
     1621 attagccagc tttgcgccta tcattcatgg cgttgccaat accaacgcca aagtcactat
     1681 tacgcaaggt ggctataaga tttatgaaac gacggtgccg ccaggcgctt tcgtcattga
     1741 tgatctgagt ccatcagggt acggcagtga tcttattgtt accatcgaag aatccgatgg
     1801 ctcaaagcgg acattctcgc aacctttttc atcggtcgtt caaatgttac gccctggcgt
     1861 tggacgttgg gatattagcg gcggccaggt cttaaaagat gatattcagg atgagcctaa
     1921 tttatttcag gccagctact actacggtct gaataactat ctgacgggtt ataccggtat
     1981 tcagattacc gataataact ataccgctgg tttgttaggt cttggtctga atacttcggt
     2041 cggtgcattt tctttcgatg tgactcactc caatgttcgt ataccggatg ataaaacata
     2101 ccaggggcaa agttatcgtg tttcctggaa caagttattc gaagaaacaa gtacttcact
     2161 gaatatcgcg gcctatcgct attcgacaca gaattatctt ggtcttaatg atgcactaac
     2221 tctgattgat gaagtgaaac atccagaaca agatcttgaa ccgaaatcca tgcgtaatta
     2281 ctcgcgcatg aaaaatcagg ttacagtcag tattaaccaa ccgttgaaat ttgaaaaaaa
     2341 agattacggt tctttttatc tttctggtag ttggtccgat tactgggctt ccggacaaaa
     2401 tcgtagcaat tactccattg gctacagtaa cagtgcatcc tggggcagct acagcatcag
     2461 tgcacagcgt tcatggaatg aggacggcga cgctgatgat agcgtttatc tcagtttcac
     2521 cattcctatt gaaaaattac ttggcactga acaacgtact tcaggtttcc agagtatcga
     2581 tactcaaatg agcagtgact ttaagggtaa taaccaactc aacgtcagta gcagtggcta
     2641 tagcgataac gctcgcgtca gttatagcgt gaatactggc tatacgatga ataaagccag
     2701 caaagatttg agttatgttg ggggttatgc cagctatgaa tcaccgtggg gaactctggc
     2761 aggttcagtt tctgcaaaca gcgataacag tcgtcaagtt tctctcagca ccgacggtgg
     2821 ttttgtattg catagtggcg gcctgacctt cagtaatgac agttttagcg actccgatac
     2881 actggcggta gttcaggctc caggtgctca aggagcgcga ataaattatg gcaacagtac
     2941 tatcgatcga tggggttatg gtgtcaccag cgctctttct ccttatcatg aaaaccgtat
     3001 cgcgctggat atcaacgatc ttgagaacga tgttgaatta aaaagtacca gtgctgtagc
     3061 tgtgcctcgt cagggttctg tcgtttttgc tgattttgaa accgtgcaag ggcaatcagc
     3121 cattatgaac atcacacgta gtgatggcaa aaatattcca tttgctgcag atatttatga
     3181 tgaccaaggc aatgtcattg gtaatgttgg acagggtgga caggcttttg ttcgtggtat
     3241 tgaacagcaa ggaaatatca acattaaatg gctcgaagaa agtaaacctg taagttgtct
     3301 tgcgcattat caacaaagtt cagaagcaga aaaaatagcg caatctatta ttctgaatgg
     3361 aattcggtgt cagattcagt aactacaagg gacgtcaaat gataaaaaca acgccacata
     3421 aaatagcgat actgatggga gtattattat caccctcagt atttgcaacg gatattaatg
     3481 ttgagtttac agccactgtc aaagcgacaa cctgtaacat cacacttact ggtaataatg
     3541 tcacgaatga tggcaataat aactacactt tgagaatccc taagatgggc ctggataaga
     3601 tcacgaataa aacgacagaa tctcaggctg attttaaact ggttgccagt gggtgcagca
     3661 gtggcattag ctggattgat accactctga ccggaaacgc attatcaagc tcatccaagc
     3721 ttattatacc gctgtctggt gatttatctt cgacgacaag taatatcggt atgggtttca
     3781 aaaaacggac tactgatgat gccactttcc ttcaacctaa caacagtgcg gacaagatac
     3841 gctggagcac agaagaaatg cagcccgata aaggtcttga aatgactgtc gcgctacgtg
     3901 aaaccgcagc agggcaaggc gtgccgggga tttttcgcgc cctggcgacg tttaatttca
     3961 tctatcaata agaaggataa ttaagatgac atttaaaaat ttacgttatg gattatccag
     4021 cagtgtcgtt ttggctgcct cattgttcag cgtaccctct tatgcggcaa ctgacagcat
     4081 tgggctgacc gtgattacca ccgtagaaat gggtacttgt accgctacat tagtaaatga
     4141 ctctgatcag gacatttctg ttgttgattt tggtgatgta tatatttctg aaatcaatgc
     4201 caagaccaaa gtaaaaacat tcaaactcaa attcaaagac tgtgcgggta tccccaataa
     4261 aaaagcgcaa ataaaattaa ccaagcgagc cacatgcgag gggactgcta atgacggtgc
     4321 agggttcgca aatggttcca cagccacaga taaagcaagt gccgtcgccg ttgaagtctg
     4381 gagcaccgaa actccggcaa cagggagagc aacacaattt agttgtgtga caccagcatc
     4441 tcaagaggta actatttcca ctgctgccaa tgcggttgtt tattacccga tgagtgctcg
     4501 cctggtcgtt gaaaaaaata aaaccgtaag taatgtcact gcaggtaaat tttctgcacc
     4561 tgccacattt acagtaacct ataactaaca gactgacaga ttcaggaact atatatgcat
     4621 cccactcaac gtaagctgat gaagagaata attctgtttc tgtcattact gtttcccatt
     4681 acctgtccag cgatcgctgg acaggatatt gaccttgttg ctaatgtaaa aaacagcact
     4741 tgcaaaagcg gaatcagtaa ccagggtaat attgatcttg gtgtcgttgg ggtgggatat
     4801 ttttcaggta atgttactcc tgaaagctat caaccaggtg gaaaagagtt cactgtcact
     4861 gtctcagact gtgcattaca gggaactggc gatgtgctaa atcagttaca tattgatttt
     4921 agagccctta gcggtgtcat ggctgcaggc tccaggcaaa tatttgccaa tgaagtgaca
     4981 accggggcta aaaatgtcgg tgtcgttctc ttttccattc aggacccggc caatatattc
     5041 aatgttctta gttcagcggg aaattctcgt tcaatctatc cagtaatgac ttctgccctg
     5101 aataattcgt catggaaatt ttatgccaga atgcaaaaaa tcgatcccgc gctggatgtc
     5161 atatctggcc aggtgatgag tcatatatta gttgatgtgc actacgaata atatatcaaa
     5221 acaaacgact gctattatga cagcagcgaa tagtgggtaa tcatgaagac attcttcaaa
     5281 tactttgttt ttttagtact atcctgctct tgctttacag cgattagtaa ggatacaagt
     5341 tttcttgttg gtaacaatgc cggggtaggc gatggtaatt tcaacggccc gagcactgcg
     5401 gctcaggtca cttttagata tgcatcaaca accaataatt tagtttttta taaacctact
     5461 caacttggcc cgacaggtgt taaattacgc tgggaacaat tagatacagc cagcggtggc
     5521 ggttttcttt actgtaatgc ctctgacagg gcaaatccag atggactaat ggatattgaa
     5581 aatgccatgg ctgattctgg taaaacctat ggtggacata agttatttaa aacctctgtc
     5641 ccaggcttgt attacactct atttattgca aaactttggt ctgcatattc aacaaaaaca
     5701 actattagcg actctggttt atatattgga gataccacac cacaaaaatt ccagtggatc
     5761 atcacagacc ctgatctaca acatatcggc tgcgataacg cgatgcggta cgatagattc
     5821 tgggccatcg gcggtgttgt acacgatctt acgattgaat tttatactga taccgatttt
     5881 aaccctacaa caaaccagga cgtatcattg tccagtaatt cgacatatct ttatgcgttt
     5941 aaggcctaca gtcccggttc tcgtgttgtt gatcacagcc atcaccttta tatcgacttt
     6001 gatctactca atgttaaatt aactaacccc acctgcttta ctgctgtact taccggaaaa
     6061 tcagtaagtg gttcgacggt aaaaatggga gaatatgcac cagggcagat taaaaatggt
     6121 gctactccgg ttccttttga tatttctctc cagaactgtg ttcgcgttgg cgatatagag
     6181 acaaagctat cttcagggaa gctgggtaca gaaaataagc aactcctggg taacactctt
     6241 actggaagcg gcgcagcaaa aggcgttgcg gtattgattg aaggattagc aaacagaaaa
     6301 agtgccctaa tgatactgaa accgaatgat tcaacttctg tttataaaga taatacgggt
     6361 caggctcagg atcacgattc agatgcaatt tacccagaag atcaaggtat aacatatcca
     6421 cttcatttcc aggcgacgtt aaagcaagac ggaaatattg ctatcgaacc aggagaattt
     6481 aaagccacca gtactttcca ggtaacctat ccctgataac attgtggcac cgtctggtaa
     6541 tggcggtgcc acaatagata aatcattgcc cccaccaatg tggctgataa aatgccagac
     6601 agcacgacat tacttatcag gatacgttat gaaaccaacc actattgctt cattgcaaaa
     6661 atgcaaacaa gaaaaaaaac gtttcgcgac catcactgct tacgattata gcttcgcaaa
     6721 actgtttgcc gaagaaggac ttaacgtcat gctggtcggc gattctctgg gcatgacggt
     6781 tcaggggcat gactccacgc taccggttac cgttgctgat atcgcctacc acactgccgc
     6841 cgtgcgtcgc ggcgcgccaa actgccttct gttagcagac ctgccattta tggcgtatgc
     6901 cacgccggaa caagcctttg aaaacgccgc aacggtaatg cgtgccggag ccaacatggt
     6961 caaaattgaa ggcggtgagt ggctggtaga aaccgtacaa atgctgaccg aacgtgccgt
     7021 tccagtttgt ggtcatttag gcctaacccc acagtcagta aatattttcg gtggctacaa
     7081 agtccagggg cgcggcgatg aagcggccga tcgactgctc agcgatgcat tagccttaga
     7141 agctgctggg gcacaactgc tggtactgga gtgcgtgccg gttgaactgg cgaaacgcat
     7201 taccgaagca ctggcgatcc cggttatcgg tattggcgca ggcaacgtca ctgacgggca
     7261 aatcctcgtg atgcacgacg cgttcggcat taccggcggt catattccta aattcgccaa
     7321 aaatttcctc gccgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt
     7381 ggagtccggc gtttatccgg gcgaagaaca cagtttccat tgaggagtga cgttgtgtta
     7441 attatcgaaa ccctgccgct gctgcgtcag caaatccgcc gcctgcgtat ggaaggtaag
     7501 cgcgtggcgc tggtgcccac catgggtaac ctgcacgacg gccatatgaa gctggtcgac
     7561 gaagccaaag cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc aatgcagttt
     7621 gaccgcccgg aagatctggc acgttatcca cggaccttgc aagaggactg cgaaaagctg
     7681 aacaaacgta aagtggattt agttttcgcg ccttcggtaa aagagatcta cccgaacggt
     7741 acagaaaccc acacctatgt tgatgttcct ggtctttcca ccatgctgga aggtgccagc
     7801 cgtccgggac attttcgcgg cgtttcgact attgtcagca agctgttcaa cctggtccag
     7861 ccggacatcg cttgcttcgg cgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg
     7921 gttgccgata tgggctttga tattgagatt atcggcgtac cgattatgcg cgccaaagac
     7981 ggtctggcgc tgagttcccg taacggttat ctgacggcgg aacaacgcaa aattgcgccc
     8041 ggtctgtaca aggttttaag ttcgattgcc gacaaattgc aggctggcga acgggatctc
     8101 gatgaaatta ttgccattgc cgggcaagaa ctgaatgaaa aaggcttccg cgccgatgat
     8161 attcagattc gtgatgccga cacattgctg gaagtctctg aaaacagcaa acgcgcagtg
     8221 attctggtag ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgaactg
     8281 gcgtaatgct taactggcgc tacggctgat ggcgccagct attcattgcc acgttatctg
     8341 taagcgatta ttcataacgc agacaactta gttaatcatt ttatcaatct cagcaagagg
     8401 taaatttgcc atctctgcaa cgtcttcccg agacattcct ttactcagaa gacgcagggc
     8461 cagtcttacc ttctggagtc cctctttata tcccttctga aatcccttct cttcaaacca
     8521 ctgcgccaac gtctgcctgg tttctctccg tgtctttatt tccatatgtt cccttaatta
     8581 tctggtcagt taacaataac aattatctgg ctataactga taatgcagat aacttagcta
     8641 atcagcttat caacctcagc aagaggtaaa tttgccatct ctgcaacgtc ttcccgagac
     8701 atccctttac tcagaaaacg cagggcgaat tcctgtctta cttcctgtct aacctcttgt
     8761 attacctcct gtcttacctc ctgtcttacc tcctgccttc ccctctcttc aaaccactgc
     8821 gccagcgtca tcatagactc ccctcccgtt tccctgtctc gcaacacacc ataaaacaaa
     8881 tccgcttgtt cagtatgacc gcgttgcagc atatagtttt gcatggcaac taactgactt
     8941 ccgctagtgt acccttcgtc aatcagcgtg accagttgct ccagcaataa cattaagtcg
     9001 cgctggcgaa tatgcttttg cagtagttcg agaatcgcaa tccgccggtg ctgcatgatt
     9061 tcgtcatccg gcgtgatggt gatatccacc agcgggaaag ggttgttgta aacgcgtcgc
     9121 gccagctccg gcgagtaaaa catatcaaac cagcacattg agagcggata aggtgtggcc
     9181 tcgccctgat aaaacagaat cggcaccacc agcggcagct tatcgtgatc cgcctccaga
     9241 tgccggtgca tggcggcaat ggcatagcgc atcatgcgaa aggtcatttt cttatccggc
     9301 ttgctttggt gttcaatcac aacatgcaga taacccgtac taccctgcat ctgtacggaa
     9361 tagagcacgt cagtgctgtg ccctttcagg ctttcttcaa taaaactccc cgactcaaga
     9421 tgcagcgtgt cgaggtcaca cagttcgcgt agctccgctg gcaaatggat atccagaaag
     9481 tcgcgagccg tttccgcatg cattaaaaat tgtttaaaca ccgcatcatg cggtgtggta
     9541 ctcggtgcat ccatctcccc tgccttccgt gttgtcttgc gtgaaggcag tatccagtca
     9601 aggtgtgccg tcgtcagccg cctctttagt gtttaccgag cagcgttcag ataaatttca
     9661 attggttaca ggcgttacat ggcacgcttc ggcacggtac gcaaactgct gatgcaaatg
     9721 ccgtcagatg agtagacact aaacaaaaat cgggcaatac tgcgtgagaa tttcctcaaa
     9781 gcaggcatcg cctgcttcgt taacgacagg gtagaaaggt agaagttatg attcgcacga
     9841 tgctgcaggg caaactccac cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt
     9901 cttgcgccat tgatcaggat tttcttgacg cagccggtat tctcgaaaac gaagccattg
     9961 atatctggaa tgttaccaac ggcaagcgtt tctccaccta tgccatcgcg gcagaacgcg
    10021 gttcgagaat tatttctgtc aacggtgcgg cggcccactg cgccagcgtc ggcgatattg
    10081 tcatcatcgc cagcttcgtt accatgccag atgaagaagc tcgcacctgg cgacccaacg
    10141 tcgcctattt tgaaggcgac aacgaaatga aacgtaccgc gaaagcgatt ccggtacagg
    10201 ttgcctgatt gttatccctg cggctggtta ctcaccagcc gcgacatcgt ctccagcgaa
    10261 tctgttctta agatataaag tcgttttagt aacaacggat tatcccccgg ttttactttg
    10321 cctttcatgg tcgtcacagc caggtgaaat ccggcttctt ttgctgcctt cacggctttg
    10381 tcattaaatc cgccaaatgg ataagaaaga tacaaaacat gtggattaaa ttgcgccaga
    10441 gcgcggcgtg aacgtgcaaa atcaaacaga atattgtgct cactacggct cagcaatata
    10501 ggccgccgat aaccatctat ccgatgcaaa aaatgagtat gtgactggaa atcaaataca
    10561 tcgcgaattt cgttaagttc agaaacgctc ataaattgca gcgattttgg gttccatttc
    10621 tgcgggtgac gtttgatgcg cgaggtaaca ataaacgccg tcgccttcat gccatattgt
    10681 ttcaacacag gatacgcata gcggctcacc gacttgaggc catcatcaaa ggtaatcacc
    10741 accgctcgcg cagggagatt gatcttattc ttcacgtaac cttccagctg cgccatgctc
    10801 agtgtcgcgt atcccctgtc gcgcagccag gccatctggt tattgaaagc gcgtaccgag
    10861 gtggtcgtcg aagtatggcg aaaacgggtg ttttcttcat cgcgcaaaat atggtgatag
    10921 gttagcaccg gcaggccatt atcgggttgg gcatccagcg cgctgatata ggccagtcga
    10981 tcgccaatac ggatctgata ccaggtttga tttaacctgt ctttcagttt atgcaaaatc
    11041 gggtagcgca aattgtccgc cagtactcca aatggcgcac tgcccacact cggcgcgtta
    11101 tagacctgcg tatctttcca ggtaactaag ttctgattac tcagcggctt gttgaggtca
    11161 cccaaaccgt cttcaacttt ttgtcgcccc tgaaccggct cgagatgccc tttatcgata
    11221 aaccctttgc caaggccaaa attaaatacg taataacttg cggcagtggg ctccacggca
    11281 atgatttgtc cggcccgaat attccccacc gtcaccatct tgtcaccaat ttgcgcccag
    11341 accgcggcat tttcgatggt ttgcatataa cgggcaggta acgcggcact gacacttgcg
    11401 gtaaacagca tcagcaggag aataacagct tgtttgtaca tgaaaaaata accgagggtg
    11461 aaacaacgct tttattgtag caaaagcgtc atcagaatcg cgggttaata ctcatacaaa
    11521 ttatgtttga ggacgaaagg cggatttttt tgttgctggt gccaaagact actcacctcc
    11581 ggcgcaccca gttcgacaat acgctcgcga aacgctgaac tggtcatggt ttcacggcaa
    11641 gccatcatct gttcaattaa cggtaacgtg acaccagaaa tcacttcgca acgggagtgt
    11701 ttgtggctta ataatgaagc cacgcggtac ggtggcgctc cggctatatc ggttaagaaa
    11761 ataacgcctt caccagagtc cgtttcatgc aaggcatcgc acatcatgcg gctgagcata
    11821 ttagagctta atccgcgcca gaaattcacg gcccggcact gaagaagtgc cccatgtttt
    11881 ttctccagcg catccagtat ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct
    11941 aacatcgcct tcctccttag ccgaaaatca gtctaccctg accattgttg ataaagggtg
    12001 atagccgtca aaactcctct cccccaaatc cagaggagag gaaatagcct tagctacgca
    12061 aaccacgtcc acgttggatc agcgaccaac agatcaaata aaacgccaca ataaagacca
    12121 ccagtacgcc aaaggtagtg accagcggaa catcattgat accgaggaag ccgtagcgga
    12181 aaccactgat catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca
    12241 acaaagtcag ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg
    12301 gcaccaggct gatgtcatcg aacgttttag caaacacgcc gttcagcaaa cccgcaaggg
    12361 agaatagcac cgcagtgagc accagggtta aggcaacgaa tacccacgaa tgcacctgaa
    12421 acggcacaaa aaacagtgaa attgccgtca ccagaatacc aacaaacaga ccacgcgcca
    12481 cgccaccacc gacatatccg gcaataatga catgagtcgg aaccggcgct accagcagct
    12541 cttcaatatt acgctggaac ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcgt
    12601 tggtgatcac cgacatcatg atcagccccg gtacgatgaa ctgcatgtag ctgaagccat
    12661 gcatatcgcc aatacgcgaa ccaatcaggt taccgaagat aataaagtaa agggtcatgg
    12721 tgatgactgg cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg
    12781 cccagatgct ttttagcgcc acccagtaaa gatgcatcat gtgcgatctc cttgtttttc
    12841 attaaccagt gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac
    12901 ctgaatgccc tgctcactta actgcgtaaa tacgctgttg atgccctgct cacgcagcac
    12961 ttcaacttcc agcgtggcgg tatctaccag tcgatactga tagccatcga gcttcggtaa
    13021 cggacttttc ggtgcgagat cgagaataaa ggtttctgat ttcagcttcg ccagcagcgc
    13081 cttcatcgaa gtattttcca ccaactcacc gtgttgaata atgccgatat tgcggcacag
    13141 catttctgct tcttccaggt agtgtgtggt gagaatgatg gtggtgcctt tgtcgtttaa
    13201 atccttcaaa aagccccaca ttgagcggcg aagttcaata tccacgcctg cggtcggttc
    13261 gtcgagaatc agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt
    13321 catcccgcca gataacatac gcgcacgttc gttgcgtttt ccccatagat cgagttgttt
    13381 aagatacttt tcgctgcgaa tgtacgcttc tttgcgctcc acaccgtagt accctgcctg
    13441 attcaccaca atttgctgca cggtttcaaa cgggttgaag ttaaattcct gtggcaccag
    13501 acccaactga cgtttagcgt ttacgacatc tttctcgaga tcgtaaccaa atacgctgac
    13561 ccgcccggag gttttattta ccagagagct gataataccg atagtggtcg attttccggc
    13621 cccgttcggc ccgagaagcg cataaaaatc acccgcttcg acctgcaaat cgattccacg
    13681 aagcgcctga acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt
    13741 cataaatttt tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag
    13801 ttgccctata ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac
    13861 atagatacac tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc
    13921 gggttttttg agaaactggc acaagcgcaa aaaccgcgct ttctatggat tggatgttcc
    13981 gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac
    14041 cgtaatgttg ctaacctggt cattcacacc gacctgaact gcctttccgt ggttcagtat
    14101 gcagtggatg tactcgaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc
    14161 gtacaagccg cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc
    14221 cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg
    14281 gataccttgt gtgaactgaa cgtcatggaa caggtgtata acctgggcca ctccaccatt
    14341 atgcaatcag cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctacggcatt
    14401 cacgacggct tgctgcgtga tctggatgtc acagcgacta accgcgaaac ccttgagcaa
    14461 cgttaccgtc acgggatctc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc
    14521 catgccggat gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc
    14581 cgatatacgg cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc accacaaact
    14641 catccgggat cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt
    14701 tatccagcag cgtacaaatc gccaacgact tcggttcgcg caggcttaag atctcacgca
    14761 ctttcgacag tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac
    14821 ggatatcttc atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac
    14881 cgtagctgga ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt
    14941 ccgccataaa cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt
    15001 ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt
    15061 cggggatcat tacttctaca gtatgtttca tatctataac catatgattt aaaaacaaat
    15121 cacttcgcac gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta
    15181 aaactattca ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt
    15241 taaaacctat aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg
    15301 caattaacaa taccggctcg cggcgattac tcgtcacgct aacagccctt tttgcagcgc
    15361 tttgcgggct gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt
    15421 actaccctat cgctggcctt gtgatgctca gcgtcgcctg gatgctgtgg cgcagtaaac
    15481 gcgccgcgct ttggctgtac gcagccctgc tgctcggcac tatgatttgg ggcgtctggg
    15541 aagttggttt cgacttctgg gcgctgactc cgcgcagcga cattctggtc ttcttcggca
    15601 tctggctgat cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg
    15661 ccgcactggt ggtcgcactg ctgattagcg gtggtatcct gacctgggcc ggatttaacg
    15721 atccgcagga aatcagcggc accttaagcg ccgataccac gccagcagaa gccatctccc
    15781 ccgttgccga tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac
    15841 ctctgaaaca aattaacgct gataacgtcc acaagctgaa agaagcctgg gtgttccgta
    15901 ctggcgatgt gaagcagccg aacgatccgg gcgaaatcac caatgaagtg acgccaatta
    15961 aagtgggcga caccctttac ctgtgtaccg cgcaccaacg tctgtttgcg ctggatgccg
    16021 ccagcggtaa agagaaatgg cattacgatc ctgagctgaa aaccaacgag tccttccagc
    16081 atgtaacctg ccgtggcgtc tcttatcatg aagccaaagc agaaactgct tcgccggaag
    16141 tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcgt ctgattgcaa
    16201 ttaacgctga aaacggcaag ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc
    16261 aaagcaatat gccggacacc aaaccgggtc tgtatgagcc gacttcgccg ccgattatca
    16321 ccgataaaac catcgtgatg gccggttcag tcaccgataa cttctcaacc cgtgaaacat
    16381 ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct gctgtgggct tttgatccgg
    16441 gcgcgaaaga tccgaatgcg atcccgtctg acgaacatac ctttaccttt aactcaccaa
    16501 actcgtgggc accagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg
    16561 tcaccacgcc agatatttgg ggtggtaacc gcacaccgga acaggaacgt tatgccagct
    16621 cgattctggc gctgaatgcc actaccggga aactggcgtg gagctaccag accgttcacc
    16681 acgacctgtg ggatatggat cttccggcac agccgacgct ggcggacatc accgttaatg
    16741 gtcagaaagt gccgattatt tacgctccgg cgaaaaccgg caacattttt gtgctcgatc
    16801 gtcgtaatgg tgaactggtg gttccggccc cggaaaaacc ggttccccaa ggtgctgcca
    16861 aaggcgatta cgtgacccct actcaaccct tctcggaact gagcttccgt ccgacgaaag
    16921 atttgagcgg tgcggatatg tggggtgcca ccatgtttga ccagttggtg tgccgcgtga
    16981 tgttccacca gatgcgctat gaaggcattt ttacgccgcc atctgaacag ggtacgctgg
    17041 tcttcccggg taacctgggg atgttcgaat ggggcgggat ttccgttgat ccgaatcgtg
    17101 aagtggcgat tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc
    17161 caggcaaccc gatggaacag ccgaaagatg ccaaaggcac gggtacggaa tccggcatcc
    17221 agccacagta cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccgtttggtc
    17281 tgccatgtaa acagccagca tggggctata tctcagcatt ggatctgaaa accaatgaag
    17341 tggtgtggaa aaaacgtatt ggtacgccgc aggacagtat gccattcccg atgcctgtac
    17401 cggtgccgtt caatatgggt atgccgatgc ttggcgggcc aatctccacg gcgggtaacg
    17461 tgctgtttat cgccgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg
    17521 aaaaactgtg gcagggtcgt ttaccagcgg gtggtcaggc tacgccaatg acctatgaag
    17581 tgaatggcaa gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga
    17641 tgggcgacta tattgtggct tatgcgctgc cggatgatgt gaagtaacca taatgcccgg
    17701 agagatccgg gcatatttcc gaatacggtc tttttatacc gtaaacccta acatcatccc
    17761 cgtatcttca tgctccagca gatggcagtg cgccatataa gcatgttctt tcggtgcatc
    17821 gtgattaaac ttcaccagca cttcgctgac attaccttct accttaacgg tatctttcca
    17881 gcctgcgcga tgcgtcgctg gcggtttgcc attttctgac aagatacgga actgcgtacc
    17941 gtggatatgg aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta
    18001 ctgccctttc gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt
    18061 gttggcatgg tggaaatcga acttcccgct gtggttcata tgattcatat tgccgtgccc
    18121 catatggccc atcatctggc tgtgatccat cccggccatg gcctgatcgc catatttctc
    18181 catcagcatc tgcatcccca tcatatcgag cattgggtcc atagaaagtt gcagattgcg
    18241 taccgtcagc ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc
    18301 accagaggca ctaatggcaa tcggctgaat ccgcattacc ggatgcggct tgtcaaacgg
    18361 cgcaatcgcc atccccatct ggctgaccgg cagcgtcacc aggtcaaagg gtttgttatc
    18421 gttaacctcc accagcactt caaaacgctc gcccatcagc actggcagct cgttcacctt
    18481 caccggttca ggtagcagac cgccgtcgct ggcaatcaca taaagcgggc gattgtcgct
    18541 ggtggcgaaa ttgagcgagc gggcattaca gccattgagc aaacgcaggc gcagccagcc
    18601 acgcggggca gcgtgttgcg ggtagattgc accgttggtc agcaacgtat cgccaaacca
    18661 acccacagcg gcggtcatca catccagttg ataatcaatc tgcccgtctg cgttaaattt
    18721 tttatcctga acaatcaccg ggacatcatc aataccccac tgttttggca gcattaattt
    18781 caggatctcg tcatcttcaa tcaccaccaa accagccagc cccatcgcca cctgacgtcc
    18841 ggtcttgcca tgttgatgtg gatggaacca gcaggtagcg gcaggttgat caacgttcaa
    18901 cgtcaccgag cgcttgccac ctggcggaat aattccctgc gggccgccgt ccacttcacc
    18961 cggtacttcc agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tgtagatatc
    19021 aaccgttacc gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata
    19081 gccccaggta gttgcggttt tcccgccaaa ggtggactga cctgcgccaa tagttaactg
    19141 aatgcgatta cgggcatcgg tcgtgagcaa atcagggatt ggtaacgttg ggcgttccgc
    19201 cgcaaatact gcgcggctcc acagcggcaa ggctgaagcc acccccagcg cgacggaata
    19261 tttcaagaaa tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat
    19321 caaacgttga gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc
    19381 gacaataatg gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg
    19441 gtagaagcaa tgaagacgtt tttcagaaca gtgttattcg gcagcctgat ggccgtttgc
    19501 gcaaacagtt acgcgctcag cgagtctgaa gccgaagata tggccgattt aacggcagtt
    19561 tttgtttttc tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc
    19621 gcgctggtct ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac
    19681 atgaaagccc tcggtgaaga cagctaccgc gatctcagtg gcattggcat tcccgtcgct
    19741 aaaaagtgca aagccctggc ccgcgattcc ttaagcctgc ttgcctacgt caaataatcc
    19801 ctgatacctt tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt
    19861 gcgccatttt tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag
    19921 tggcatgaaa cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat
    19981 catgaaaaga ccgatcacca ggatctgatc atttttgaga acgccgcatt tggtcgcgta
    20041 atggcgctgg atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg
    20101 atgacccatg ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc
    20161 ggcgacggtg ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg
    20221 gtggaaatcg atgcgggtgt cgtgtcgttc tgccgtcagt atcttcccaa ccataacgcc
    20281 ggtagttacg acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa tttcgttaat
    20341 caaaccagcc agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc
    20401 gaaagtctgt tcacttcggc attttatgaa ggctgcaaac gttgcctgaa tcctggcggt
    20461 attttcgttg cgcagaacgg cgtctgcttt ttacagcagg aagaggccat cgacagtcat
    20521 cgcaaactca gccattactt cagcgatgtt ggcttttatc aggcggcgat cccgacctat
    20581 tacggcggca tcatgacctt tgcatgggcg acagataacg acgccttacg ccatctttca
    20641 accgaaatta ttcaggcgcg ttttctcgct tctggcctga aatgccgtta ttacaatccg
    20701 gcagtccata cggcagcttt tgccttaccc cagtatctgc aagatgcact ggcttcacag
    20761 ccgtcctaag gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg
    20821 accaaaagtc tgagtttttg tatttacgat atctgctacg ccaaaactac cgaagagcgc
    20881 gacggttata ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg
    20941 tcagaaacct gttccattat cggggccaat attcttaaca tcgcccgcca ggattacgaa
    21001 ccacaaggtg ccagcgtcac tattctggtg agtgaagaac cggtcgaccc gaaacttatc
    21061 gacaaaacag aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt
    21121 catatttgcg tacataccta cccggaaagt catcctgaag gcggtttatg taccttccgc
    21181 gccgatattg aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg
    21241 atccaccagc ttgagtccga tattgtgact attgattatc gcgtgcgcgg ttttactcgc
    21301 gacattaacg gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg
    21361 tctgacgata tgaaggcgct gtatgacatg gtggatgtga acgtctatca ggagaatatc
    21421 ttccatacca agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa
    21481 ccggaagatt taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg
    21541 cgcgagattt attacgggcg caatatgccc gctgtttaac gtctcaggcg gagcacggag
    21601 gctccgcctg atttacttac ttctgctgca cgaaattacg gtaagccgcc accacctgaa
    21661 gaaaatcctc aacgccacac agtgacaagc tctcttcatc gtagtagttc atcccctctt
    21721 ccatttcatc gccagcgaat tccagttgat tggcgcgaac catcacctct tcaccatcca
    21781 tccatagcgt gtattcatgc cctgcccgtt gccaggaacg ttcgctacct ttcaatgcgt
    21841 gcgccgcttg ttccacttca tcaagcaagg ccaggttttc tttcacctct tcattaaacc
    21901 agtgcccgac cacttcatgc cccatggaca tgcgcacctt taccactccg gtaatatcgc
    21961 gcagaaattc gtaatccata gtgtgttcct ctgctcccgg caatgcgccg aaatcgcatt
    22021 gcgccattga tgtaattatc gcagtcttac cggagaaaaa cagcggaacg gcgatggttt
    22081 agaaataaaa aatgcccggg aatctcattc accgggcatt gtgtcgttta tgcgcagcgc
    22141 gtgcgcttac ttattaaacc gcagtctgga aaatcacccc gtccgctttt tcggtgtact
    22201 gagaaagctg gtcgaagttc agataacggt aagtatcaac ggcagtttta tctacttgcg
    22261 ccacgtaggt ctggtactct tccggcgtcg gcagtttgcc aatcagcgcc gcaacagcag
    22321 ccagttccgc agaagccagg aagacattcg cgccagtacc caaacggttc gggaagttac
    22381 gggtagaggt ggaaaccacc gtcgcaccgt ctgctacacg cgcctggtta cccatacaca
    22441 gggaacagcc agggatctcg atacgagcac cactcttacc gaagacgctg tagtagcctt
    22501 cttcggtcag ctgcgcagcg tccatacgcg ttggcggtgc cacccacagg cgggtcggca
    22561 actgaccttt gtgcgcatcc agcagtttac cagcagcacg gaagtgaccg atgttagtca
    22621 tgcaagaacc gataaatact tcgtcgatct tctcgccctg taccgcagac agcggacgtg
    22681 cgtcgtccgg gtcattcggt gcacagagga ttggctcttt gatatccgcc agatcgatgt
    22741 cgatcactgc cgcgtattcc gcatctgcat cggcttccag cagctcagga ttcgccagcc
    22801 atttttccat gccctgaata cgacgctcca gggtacgacg atcgccgtaa ccttccgcga
    22861 tcatccactt cagcaggacg atgttagagt tcaggtattc aatgatcggt tctttgttca
    22921 gcttgatggt acaaccggca gcagaacgct cggcagacgc atcggtcagc tcaaatgcct
    22981 gctcaacttt cagatccggc agaccttcaa tttccagaat acggccagag aagatgtttt
    23041 tcttaccttt cttctcaacg gtcagcagac cttgtttgat tgcatacagc ggaatggcgt
    23101 gtaccagatc gcgcagggtg atacccggct gcattttgcc tttgaagcgt accagaacgg
    23161 attccggcat atccagcggc attacgcctg ttgcggctgc aaatgccacc agaccagaac
    23221 ccgccgggaa agagataccg atcgggaaac gggtatggga gtcaccaccg gtaccgacgg
    23281 tatccggcag cagcatacgg ttcagccagg agtgaatgac gccgtccccc ggacgcagcg
    23341 acacaccgcc acggttcata atgaagtccg gcagcgtgtg gtgcgtgttc acgtcaactg
    23401 gcttcggata cgccgcggtg tggcagaaag actgcatcac caggtcagcc gagaagccca
    23461 ggcacgccag gtctttcagt tcatcacggg tcatcgggcc ggtggtgtcc tgggaaccta
    23521 cagaagtcat tttcggttca cagtacgcac ccggacgaat gcctttcacg ccacaggcac
    23581 ggcctaccat tttctgcgcc agcgagaagc cgcgatcgct ctcagcgaca tctttcgcct
    23641 gacggaacac atcactatgc ggcaggccaa gtgcttcacg cgctttggtg gtcaggccac
    23701 gcccgataat cagcggaata cggccgccag cacgcacttc atcaatcagc acgtcggttt
    23761 tcagttcgaa ggttgccagc agttcgccgg tttcgtggtt acgcacttca cctttgtacg
    23821 ggtaaacgtc aattacgtcg cccatgttca ggttagagac gtcgacttcg attggcagtg
    23881 cacccgcgtc ttccatcgtg ttaaagaaga ttggtgcaat tttaccgccg aggcacaaac
    23941 caccgccgcg tttgttcggc acatgtggaa tatcatcgcc cataaaccac agaacggagt
    24001 tcgtggcgga tttacgcgat gaccccgtac ccacaacgtc accgacgtac gccagcggga
    24061 aacctttctg ttgcagagct tcaatctgtt tgatcgggcc aacaacacca ggctgatctg
    24121 gctcaatacc ttcacgggcg tttttcagca tcgccagcgc gtgcagtggg atatccgggc
    24181 gtgaccacgc atccggtgcc ggagaaaggt catcggtgtt ggtttcgccg gtcactttga
    24241 agacggtaac ggtcagtttt tcagccagcg ccgggcgatt caggaaccat tcggcatccg
    24301 cccaggactg cattacctgc ttcgcatatt cgttacctgc tttggctttc tcttctacgt
    24361 catagaagtt atcgaacatc agcagtgtgt gagaaagtgc tttggcagcg atcggtgcca
    24421 gtttggcatc atccagcgcg tcgatcagcg gatgaatgtt gtaaccaccc tgcatagtgc
    24481 ccagcagttc aatggctttt tccggagtca gcagtgggga tttggcttca cctttggcga
    24541 cagcagccag gaagcctgct ttgacatagg cggcttcatc gacgccaggg ggaacacggt
    24601 tggttaacag atctaacagg aattcttctt cgcccgcggg cgggtttttc agcagctcta
    24661 caagcgcggc catttggttt gcatccaggg gtttgggcgc aatcccctca gcggcacgct
    24721 cagctacgtg cttacggtat tcttctagca cgacggttct cctcgctctc attgtcatag
    24781 tgcggcaggc gattctcttc acgctcctgt gagacagcag tttgtagggt aaatgcccag
    24841 taccgcaacg ggcagaatag caggattttg acgatgtgtt aatctgttta caaaaaagca
    24901 acataaaaaa gtggctgaat cgttaaggat ggtctagaga ttatttccct ccgtaaaaac
    24961 ttcgttaatt cgcatggtga taatcacctt tctcaacgaa tcccaagatt agtcaaaatt
    25021 taaactacgg cctctttata ctcggattca cagcacctgc gggtggcagt tcgccgacca
    25081 ttgcgatttc cttgagatcc gaattatgaa aatgactttg ccgtttaaac cccatgtgct
    25141 ggcgctaatt tgcagtgccg ggctctgtgc cgcctctgcc gggctatata taaaaagccg
    25201 cacagtggaa gcgcctgtgg aagcgcaatc gacacaacag actgcgcctg acatctccgc
    25261 agttacgctt ccagcaacgg tttccgtgcc tcctgtaaca cctgccgtcg tcaaatccgc
    25321 attcagcact gctcaaatag atcaatgggt tgcacctgtc gcgctatacc ccgactctct
    25381 gctttcacaa gtgttaatgg catcaaccta tccggcaaac gttgctcaag cagtgcaatg
    25441 gtcgcacgat aatccactta aacaaggcga tgctgctatt caggcggtat ctgaccagcc
    25501 ctgggacgcc agcgtcaaat cactggtggc ctttccacag ttgatggcat tgatgggcga
    25561 aaacccgcaa tgggtgcaaa acctgggcga tgcttttctg gcgcagccgc aggacgtgat
    25621 ggactcggta cagcgattgc ggcaactggc gcaacaaacc ggctcgctga agtcatcaac
    25681 tgaacaaaag atcatcacga caacgaaaaa agtcgtaccg gtaaatcagc ccgccaacgc
    25741 ccctgccaca caatcaaata ccgtttctac gtccagcccc gtcgtcgcag aacctgcacc
    25801 gaccgtaata accattgagc cagccaatcc ggatgtggtt tatattccga actacaaccc
    25861 aaacgtggtt tacggcagtt gggccaatac cgcttatcca ccagtttatc tgccgccgcc
    25921 agccggagaa ccgtttgttg acagctttgt gcgcggattc ggctatagca tgggcgttgc
    25981 taccacatac gcactattca gcagcatcga ctgggacgac gacgatcatg accatgacca
    26041 tcatcatcat gacgatgatg attatcatca ccacgatggc ggtcatcgtg acggtaatga
    26101 ctggcaacac aacggcgaca acatcaatat cgacgtcaac aatttcaacc gtatcaccgg
    26161 tgagcatctt actgataaga atatggcatg gcggcacaat ccaaactacc gtaatggtgt
    26221 gacctatcat gatcaggata tggcaaagcg gtttcatcaa accgatgtca acggcggaat
    26281 gagcgccacg cagttacctg ctctatcgcg cgacagccag cgtcaggcgg cagcaagtca
    26341 gtttcagcaa cgaacacacg ccgcccccgt cattacgcga gatacccaac gtcaggcagc
    26401 ggcacagcgg tttaatgaag cggaacacta tggcaattat gacgacttcc gcgagttcag
    26461 ccgccaccaa ccactgaccc agcagcaaaa ggacgccgct cgccagcgtt atcagtctgc
    26521 ctcacctgag cagcgccagg cggcccgtga gaaaatgcag actagcccgc agaatcaaca
    26581 gcaaagagat gcagcacgtc agcgtattca gtccgcgtcg cctgagcagc gccaggtgtt
    26641 taaggaaaaa gtacagcagc gcccactgaa ccaagagcaa cgtgataacg cccgccagcg
    26701 tgttcaatcg gcatcacctg aacaacgtcc gttttttcgg gagaaagttc aggagagccg
    26761 cccgcaacgt ctaaacgaca gtaaccgtac tgccaggctg aataacgagc agcggtcagc
    26821 agtacgcgaa cgtctctctg aacgtggggc aagaagactg gaaaggtaaa ttgcagacgt
    26881 aaaaaaagcg gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaaaaat
    26941 tacttcttct tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc
    27001 gccaggccca cagactcgtg cagagtcggg tgcgcatgga tggtcagtgc gatgtcttca
    27061 gcgtcacaac ccatttcgat tgccaagccg atttcaccca gcaactcgcc gccgttagtg
    27121 ccgacaatcg caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata
    27181 ccgtctgcgc agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt
    27241 tcgtagctga tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct
    27301 ggttcggtat aggcgatgga cgggataact ttcggatcga agtaatgttt tttaccggcg
    27361 ataacttcag cggcaacgtg accttcgtga acacctttgt gtgccagcat tggctgaccg
    27421 acgatatcgc cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg
    27481 atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc
    27541 ggcacacgac caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt
    27601 ttgccttcca tcgtcacata aatgccgtct tctttcgctt caacggcggt aactttggtt
    27661 tccagcatca ggttgaattt cttgctgata cgcttggtaa agactttaac gatgtcttta
    27721 tcagctgccg ggatgacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg
    27781 tggtatacgg tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct
    27841 ggtacttctt tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga
    27901 ataaacggca gttgaatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc
    27961 acggttttac cgttctcacc ttcaacttcc agggtgttag ccccggtgaa tttacccaga
    28021 ccgttgacca ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga
    28081 ttgatcactt tctctttcca ggtacgaatc ttgtcgatat cggttttcgg ttcgccgaag
    28141 acgataccgt gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt
    28201 gctttagaag ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct
    28261 acgattacgg tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg
    28321 cctgccccaa gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat
    28381 ttatctccgg cgggtctgac gtttttttta tgacgcccat tatccaccgg gtcgttctat
    28441 ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc
    28501 tattcgctaa taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc
    28561 ggctctttta cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta
    28621 atgaaacggg caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc
    28681 agcatcagac gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa
    28741 acgccgagga tagccacttc cggcgcattc acaatcggcg cgaagtgggt agtacccagg
    28801 ccgccgatgc tcgagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg
    28861 tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg
    28921 ttgacgtctt tgaataccgg gacaaccaga ccgttcgggg tatccaccgc cacaccgatg
    28981 ttgatgtatt tcttcagggt cagacgctga ccgtcttccg acagcgaact gttgaagcga
    29041 ggcatctgct caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc
    29101 acatccagct tacgtttcgc tgcttcttcg ttctgctgtt tacggaacgc ttccagctca
    29161 gtgatatcgg ttttgtcgaa gtgagtaacg tgcgggatca tcacccagtt acggctcagg
    29221 ttagcaccag agattttctg aatgcggccc agttccactt cttcgatttc accaaacttg
    29281 ctgaagtcca ctttaggcca cggcagcatg cctgggatac cgccgccagt tgccgccgga
    29341 gctgcttctg cacgtttgat tgcttctttc acgtaagcct gaacgtcttc gcgcaggata
    29401 cgacctttac ggccagtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga
    29461 cggcggatca gcggagtcgc gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct
    29521 tcggctttcg cagccggtgc tgctgccggg gcttcagctt ttgctgccgg agccggcgct
    29581 gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata
    29641 atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct
    29701 gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtgat cagtgactgt
    29761 tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg
    29821 ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcagaggc cgctgccgga
    29881 gccgcttcct gtttagccgc cggagctgcc gcgccagctt cacccgcgac ttcgaagacc
    29941 ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggta
    30001 cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt gatcagcgac
    30061 tgttcagctt caactttgtc gcccactttg accaggattt cggtcacttc aacttcgtcg
    30121 ctgccgatat ccggaacgtt aacgtctttt gccgctgcag ccgctggtgc tgctgccgga
    30181 gcggcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa
    30241 tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct
    30301 ttaacgatac ccgcctgcgg agatggtact tccatagagg ctttgtcgcc ttctacggtg
    30361 atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca
    30421 acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt
    30481 acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa
    30541 ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat
    30601 aagaagcatc aacttcaaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc
    30661 catcagtacc cagtacgcgg tagtcgtcag ccggtacgta agtacggacc tgctcagcga
    30721 acagtttcat atagtcggta gatgccactg ccggagcgtc gttcatcacc tgagcgatat
    30781 acggaacgcg cggagtttcc agcgggtgca gcatgttcca gcgttcacaa tcctgaccat
    30841 cacgcgccag ttcggtgaag gaagtcacgc tataaacgtc agaacctacg ccgtaatctt
    30901 tcgccaggat ctctgccgct tcacggacgt gacgcaggat agaaccggag cccagcagct
    30961 gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt
    31021 cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta gtgatgtagt
    31081 agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc atgatgacag
    31141 caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa
    31201 tatggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag
    31261 taccgccgat caggaagcca cgcgcttgct ggtcgccagc cgcccagcac agatcgccga
    31321 tacgctggaa accgaacatc gagtaataga tgtagaacgg gatcatcggc agattgttgg
    31381 tgctgtaaga ggtcgccgct gccagccagg aacaacctgc gcccagctcg ttgatccctt
    31441 cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg
    31501 gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac
    31561 cgaaggtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct
    31621 tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagatctct ttgctctgct
    31681 cttccagcag cgcgccgaaa tcttgcaggc tcggcagctc cagcttctcg gtgaagttcg
    31741 gctggcggct tggcagataa ccgtgcagtt tctgacgttg agcgtgcaga taggtatgct
    31801 cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg
    31861 gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc ttaacctggt
    31921 gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa
    31981 ggattaccgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct
    32041 tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca
    32101 gtgctgcggt ttcaggatat ttaccgaaga agtgttcgcg aacatacgca ccatctttcg
    32161 atttgaaggt ctggtaatcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc
    32221 tggtatcttt acgcagcagt tcgtcccaac ggctacccca catcactttg atcacgttcc
    32281 agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg
    32341 ggccgtcaag acgctgcagg ttacagttga taacgaagac cagattatcc agtttttcac
    32401 gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga
    32461 acgcgtaaac ggtttgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga
    32521 atttagcctg gtaaatagca ccaatcggac ccagccccat agatacggtc gggaactgcc
    32581 agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct
    32641 gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca cgagcgtaaa
    32701 cgcctgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg
    32761 cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca
    32821 tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg atggcgttcc
    32881 agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt
    32941 cttcaacggg gatggtgttg atgtagttgc tgataccggt gcctgcagct acgttgactc
    33001 cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt
    33061 cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt
    33121 catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct
    33181 ggaacctgtc ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt
    33241 gccgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat
    33301 cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct ctcttcacgg
    33361 cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct
    33421 tcttccggct taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc
    33481 ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgagccaac
    33541 atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg
    33601 gcaatctgat actggagtac ggcatttgat tccgcgtcca gatcgccaga ctgctgcgcc
    33661 agctctatgg catggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc
    33721 gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt
    33781 gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg ccagaggctg
    33841 ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgaggcgt
    33901 tgaatcgcct cacgcaaaga gggacgggag acgtcgaact gttttgccag ttcgcgttcc
    33961 ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc
    34021 tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt
    34081 aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca
    34141 aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta
    34201 acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtta aatttaccca
    34261 tcaaggggtg accttaacag aattgaaacg aaaaaatatt tcctctgaac cgattcacca
    34321 agcaacaaaa cgcataacaa ttcaattatg tgaattaata t
//
LOCUS       DAPQZD010000036        34116 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPQZD010000036 DAPQZD010000000
VERSION     DAPQZD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.036"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(128..268)
                     /locus_tag="RO454_001685"
     CDS             complement(128..268)
                     /locus_tag="RO454_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933532.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            364..1080
                     /gene="arcA"
                     /locus_tag="RO454_001686"
     CDS             364..1080
                     /gene="arcA"
                     /locus_tag="RO454_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDY0933533.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1140..2492)
                     /gene="creD"
                     /locus_tag="RO454_001687"
     CDS             complement(1140..2492)
                     /gene="creD"
                     /locus_tag="RO454_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDY0933534.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDLRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLVLLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(2550..3974)
                     /gene="creC"
                     /locus_tag="RO454_001688"
     CDS             complement(2550..3974)
                     /gene="creC"
                     /locus_tag="RO454_001688"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDY0933535.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3974..4663)
                     /gene="creB"
                     /locus_tag="RO454_001689"
     CDS             complement(3974..4663)
                     /gene="creB"
                     /locus_tag="RO454_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDY0933536.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFGVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4676..5149)
                     /gene="creA"
                     /locus_tag="RO454_001690"
     CDS             complement(4676..5149)
                     /gene="creA"
                     /locus_tag="RO454_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDY0933537.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5360..6229
                     /gene="robA"
                     /locus_tag="RO454_001691"
     CDS             5360..6229
                     /gene="robA"
                     /locus_tag="RO454_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDY0933538.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6226..6873)
                     /gene="gpmB"
                     /locus_tag="RO454_001692"
     CDS             complement(6226..6873)
                     /gene="gpmB"
                     /locus_tag="RO454_001692"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDY0933539.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6925..7452
                     /gene="yjjX"
                     /locus_tag="RO454_001693"
     CDS             6925..7452
                     /gene="yjjX"
                     /locus_tag="RO454_001693"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDY0933540.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="RO454_001694"
     CDS             complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="RO454_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDY0933541.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="RO454_001695"
     CDS             complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="RO454_001695"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDY0933542.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10091..11758
                     /gene="ettA"
                     /locus_tag="RO454_001696"
     CDS             10091..11758
                     /gene="ettA"
                     /locus_tag="RO454_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDY0933543.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11879..13111)
                     /gene="nadR"
                     /locus_tag="RO454_001697"
     CDS             complement(11879..13111)
                     /gene="nadR"
                     /locus_tag="RO454_001697"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDY0933544.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13132..14514)
                     /gene="radA"
                     /locus_tag="RO454_001698"
     CDS             complement(13132..14514)
                     /gene="radA"
                     /locus_tag="RO454_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDY0933545.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14563..15531)
                     /gene="serB"
                     /locus_tag="RO454_001699"
     CDS             complement(14563..15531)
                     /gene="serB"
                     /locus_tag="RO454_001699"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDY0933546.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMSGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15637..16281
                     /locus_tag="RO454_001700"
     CDS             15637..16281
                     /locus_tag="RO454_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDY0933547.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16309..17325
                     /gene="lplA"
                     /locus_tag="RO454_001701"
     CDS             16309..17325
                     /gene="lplA"
                     /locus_tag="RO454_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDY0933548.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFQEQEKELRELSAWMA
                     GAVR"
     gene            complement(17326..18657)
                     /gene="yjjJ"
                     /locus_tag="RO454_001702"
     CDS             complement(17326..18657)
                     /gene="yjjJ"
                     /locus_tag="RO454_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HDY0933549.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVARENRVIRFGK
                     ARATRYVLLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTDDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     EGNYQRWITAQRPAEIPLDQKLTRYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRHLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNIS
                     KSAWLTAIPLAQQFWQTVARDPRISEAFRHIAQEMPEKIRQIEEKVTRMGG"
     gene            complement(18824..19543)
                     /gene="deoD"
                     /locus_tag="RO454_001703"
     CDS             complement(18824..19543)
                     /gene="deoD"
                     /locus_tag="RO454_001703"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDY0933550.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19600..20823)
                     /gene="deoB"
                     /locus_tag="RO454_001704"
     CDS             complement(19600..20823)
                     /gene="deoB"
                     /locus_tag="RO454_001704"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDY0933551.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20875..22197)
                     /gene="deoA"
                     /locus_tag="RO454_001705"
     CDS             complement(20875..22197)
                     /gene="deoA"
                     /locus_tag="RO454_001705"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDY0933552.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEHRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(22275..23054)
                     /gene="deoC"
                     /locus_tag="RO454_001706"
     CDS             complement(22275..23054)
                     /gene="deoC"
                     /locus_tag="RO454_001706"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDY0933553.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            23312..24862
                     /locus_tag="RO454_001707"
     CDS             23312..24862
                     /locus_tag="RO454_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDY0933554.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNVCECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLIDPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24834..25697
                     /locus_tag="RO454_001708"
     CDS             24834..25697
                     /locus_tag="RO454_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDY0933555.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSSGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(26213..26995)
                     /locus_tag="RO454_001709"
     CDS             complement(26213..26995)
                     /locus_tag="RO454_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDY0933556.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26992..28065)
                     /locus_tag="RO454_001710"
     CDS             complement(26992..28065)
                     /locus_tag="RO454_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDY0933557.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTCHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(28187..28348)
                     /locus_tag="RO454_001711"
     CDS             complement(28187..28348)
                     /locus_tag="RO454_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDY0933558.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(28475..29080)
                     /gene="osmY"
                     /locus_tag="RO454_001712"
     CDS             complement(28475..29080)
                     /gene="osmY"
                     /locus_tag="RO454_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDY0933559.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(29473..31062)
                     /gene="prfC"
                     /locus_tag="RO454_001713"
     CDS             complement(29473..31062)
                     /gene="prfC"
                     /locus_tag="RO454_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDY0933560.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(31153..31830)
                     /gene="yjjG"
                     /locus_tag="RO454_001714"
     CDS             complement(31153..31830)
                     /gene="yjjG"
                     /locus_tag="RO454_001714"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDY0933561.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31845..32291)
                     /gene="rimI"
                     /locus_tag="RO454_001715"
     CDS             complement(31845..32291)
                     /gene="rimI"
                     /locus_tag="RO454_001715"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDY0933562.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(32260..32673)
                     /gene="holD"
                     /locus_tag="RO454_001716"
     CDS             complement(32260..32673)
                     /gene="holD"
                     /locus_tag="RO454_001716"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDY0933563.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32776..33807
                     /gene="rsmC"
                     /locus_tag="RO454_001717"
     CDS             32776..33807
                     /gene="rsmC"
                     /locus_tag="RO454_001717"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDY0933564.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLNRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            34007..34093
                     /locus_tag="RO454_001718"
     tRNA            34007..34093
                     /locus_tag="RO454_001718"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34041..34043,aa:Leu,seq:cag)
BASE COUNT         7978 a         9405 c         8750 g         7983 t
ORIGIN      
        1 taaaataatg tgacgaaagc tagcatttag atacgatcat ttcatcaaac tgttaacgtg
       61 ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc ctgcccctct
      121 tagcctgtta tattgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt
      181 cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat
      241 caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca
      301 tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca
      361 aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg
      421 aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg
      481 catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt
      541 aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc
      601 ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac
      661 tacatcacca aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa cctgctgtcc
      721 cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc
      781 aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac
      841 aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggtaaaatt
      901 cagtcccgtg ctgaactgct gaagaaaatg accggccgtg agctgaaacc gcacgaccgt
      961 actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg
     1021 gaaatcatcg ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa
     1081 tcggctttac caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacat
     1141 tatttccaga tacgtaactc atcgtccatg gtaacttctt tactggcttt cattttcggc
     1201 agtgaaaacg cataccagtc gatattacgg gtcacaaaca tcatgccggc cagcgccacc
     1261 accagcacac tggttcccaa caacagcgcg ctatcggcag agttgagcag tccccacatc
     1321 acaccatcca gcaacaacag cacgagggta aacaacatgc tattacgcca gcctttcaat
     1381 accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc
     1441 acagtaaaac cggtatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa
     1501 agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca
     1561 aagaatgcca taaaagtcag cgcaatcagc agaatggcgt acttagtcgc ccggtccgtt
     1621 aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag
     1681 ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca gctactttgc
     1741 cattgcgctt gaaaacctga ctcgctaact tcccgtttgg ctggtagaaa atcacctaaa
     1801 aaactgggat gcggccagtt gctggttaag gtcatttcgc tattacgccc gccaggcacc
     1861 acagaaagat cgccggtacc gcttaaattc agcgccacat tcagcttcag gttctgcttg
     1921 cgcaagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggtg
     1981 ccgggttcaa tggtcagcgc cgtaccgtta acttcaggcg ctttcaccac accaatacca
     2041 cgcgcatccc cgacgctaat cacaataaat ggcttgccta aggtgatatt tggcgcgtcg
     2101 agttcgctaa gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc
     2161 tgaccggtat agatgcctat cttgcgttct tccacattct gattgccatc aaccatcaat
     2221 gactcaggta accaaaaatg aataaaactt cgtttccgct ccacggtttt atcgtcttcc
     2281 tgcaccgtat aaagctcggt cacaggaata gcgatgagcg gcccaacgag tttttgcggc
     2341 ccgctggtac tttggcgaat cacatcttcc acatcgctac ggtaatcggc acgttcgaca
     2401 atcacctgcc gaatcagcat tatcggaatc aacaacagca atactgcacc aaacaaggtg
     2461 gtcattttcc agaacagggg ggatttcaac atagtcttct cctttgcaat gacagcagga
     2521 tacgaaggct atgtgggaag aatttgaagc tatgtgaagt gacggtgaag tcgaagcgag
     2581 gccagcacgc caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg
     2641 acctcactga caaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa
     2701 gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag gaatcccact accggtatcc
     2761 agcaccttaa gggtgacgta ttcctgatcc acttcggcgc ttagcgttat gcgaccgctc
     2821 tcgggagtaa aatcgatggc gttatccagt aaattcccca gcgcctgctc cagtaacgcc
     2881 ggttcagcag caacgttaat ctcagtaggc ataacatgca aagtgatgtt tttttctgcc
     2941 aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacagcagtc
     3001 agaacgactt cctgacgatt ctccagtctt gcctggcgta gtaacgtttc caccagtgcc
     3061 tgcattcgcg cattttgcgt cagaatgttg tcggtaaaac gagccaccac ttccggcggc
     3121 ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca
     3181 tgagttaacg cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata
     3241 ctttccagcg cctgcgcgag tttacgcagc tcgctactac cgagatcggg gagaggaacg
     3301 ggcttattgt cagtgacgga atcagcatag cgagtgagcc gggcaataga gcggttgatc
     3361 caccaaacca tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc gctggcccat
     3421 aaaattcgcc gctcgctacg cttaatgacc ggggccatcg ccgcgttcgg tttgcctacg
     3481 ctcaaaacgc caataagccg cgagccgtcc ataatcggcg cggcaacata catcacagaa
     3541 ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt
     3601 agccagacgt cattccagcg cgaataatcc tgtccaacgg ctttatttgc cgaatcaaac
     3661 aatactttgc cctgcgcatc ggtcatatag acatgatatt cgttgcgcac tttgttaatg
     3721 ccaccgatat tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc
     3781 ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg
     3841 gtgtcgatta acgtcccctc ggttgctctt cgcacgcccg gtttaacttc tttgacaaaa
     3901 atagccagta cgaaccaggc cgccaccgcc accagtaaaa aatagcccag caacaaccgc
     3961 atgccgatac gcattacagg cccctcaggc tatatcccat gccgcgatga gtattaatcg
     4021 gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag cgttttaatg tgggtatcga
     4081 cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg
     4141 accagacgcg gcccggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca
     4201 gcgttaatgg cgtgtcaaac cagctgatct gcgccgcagg ttcattcaat tcaaaatgac
     4261 caatacggat aacgggagac ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca
     4321 ctctggcgca cacttcgcga ggtgaaaacg gtttagccac gtagtcgtca gcgccaattt
     4381 ccagcccaag caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg
     4441 ccggatggag cgccagtaac tggcggcaca attcaaagcc gctgatatca ggcaaaccaa
     4501 catcgagaat catgacatcg ggaacctgct ggcgagcttt atccagcact ggcaagcctc
     4561 gctcaaagac ctcgacgcca aaaccttcct gttgcaacat gtagaccagc gtatcggcta
     4621 tcccttgctc gtcttctact aaccagaccg tttcccgttg cataaatcgc ctctgttatt
     4681 gccgccacgg catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt
     4741 tgtcggagta agccagataa gcgagcgcgt tgcgtttagc gtcataaaag cgcacgacct
     4801 gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc
     4861 cgtttttaat acgatccgac agttcaatcg gcccgacttg ttgacaagaa atggccgcat
     4921 cggaggtatc ttctgccagg cccaatcccc ctttaatacc accggttttc gcccggctca
     4981 cataacaggt gacatttttc acatcgggat cgtcaaaggc ttcaacaaca attttgtgat
     5041 ccgggccgat cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg
     5101 gccccagcat tatcaggctt aaagaaagga ttaaatgctt gtatttcata ttgttaccat
     5161 ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa
     5221 aatctcaata cttttatttc cgtcaagccc taaaacatac tcgactaaga aaaaaacact
     5281 gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt
     5341 gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct
     5401 ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc
     5461 caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat
     5521 tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct
     5581 ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa
     5641 gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat
     5701 tcgcccgccg ctgcgtctgg gtgaattcac tatgccagag cacaaatttg tcaccctgga
     5761 agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga
     5821 tttccgccat gaaatgcgct accagttctg gcacgatttt ctcggcaacg cgccgaccat
     5881 tccgccggtg ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgatgaaca
     5941 agaggtattc tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg
     6001 gcatccggtg atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac
     6061 cggcgtgcag gagtttatcc tgacggtata tggaacatgc atgccaatgc tcaacctgac
     6121 gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagatgcca aagcgggaga
     6181 tcgcccaatt aatctgcgct gtgaactgct gattccgatc cgtcgttaac gctgcagctc
     6241 atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacca cccagccaga
     6301 cgccagccac aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa
     6361 gcggcgctct gcccatgctg gtaatccgag aatcgtactc accaggcatc ccagtgcaat
     6421 accatggctc accagtagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc
     6481 cgcattaacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac
     6541 ggtgccattg accagttgcc gacgccagtt ctcttcttct tcggtgagtg aatcgatatt
     6601 tcttgtttcc agtacaccca tatttaattc acgcaggcga gaatcaaata tgatgtcaca
     6661 gccgcaggcc tgggcgatga tttccgccgt gcgccgggtg cgtcctaaat cgctactgat
     6721 gatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc
     6781 tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt
     6841 ttcaccgtgg cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg
     6901 ttaattttct gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct
     6961 aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt
     7021 gcatccgttg ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc
     7081 gctggcgcac gaaatagggt agccaatgcc cgccgtttac ttcctgaggc tgatttttgg
     7141 gtggcgattg aagctggcat cgatggtgac agcactttca gttgggtggt gattgaaaac
     7201 gccagccagc gcggcgaggc gcgttcggct actttgccgt tgcctgcggt gattctggaa
     7261 aaagtgcgtg aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag
     7321 attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcaccagc
     7381 gtctatcacc aggcggtgat cctggcgctg agtccgtttc ataatgccgt gtatcagcca
     7441 ttgcaggcct gattaggcgt tcacgccgca tccggcattc ggtgcacgat gcctgatgcg
     7501 acgctgacgc atcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cgtcttccag
     7561 ccactggcgc agctcgacgg gcgcggcttt cagactatta gatccacgcg taatcgtcgc
     7621 gatgcccgcg ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc
     7681 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
     7741 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
     7801 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccatagt
     7861 atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt
     7921 acgatagcat atcataaacg tgtggatcag taacgacgtc cccattccgt ggcgctcatc
     7981 aacgtcggtt tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgtttttc
     8041 acataaccgc gcgtctcgga gaatggaata ctctcgacaa atgccactgc gtcgatacgc
     8101 cctgcgctgt tgccaagcca ggtccgcacc cgccctggtc cggcgttata agctgctgag
     8161 gagaaaatac ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg
     8221 ttgatgttcg tttccggatc cagcaattgc ccagggctgc tataaccggg aatagagaac
     8281 atcttcaccg tatgggtcgc ggtaccaggc ataatctgca tcaggccgct ggcccctacc
     8341 ggtgatttca ctttcggatt ccaggcactc tcctgacgag caatcgccat cgcatagctt
     8401 tgcgggatct ccttaccact ggtgtagcgt ttgaaaagat cgttgtaagc cagcgggaat
     8461 cgctcttcca gatgatccca cagcttcccg gcgatcgttg cctgaacgct aagatcccac
     8521 cattggttat tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc
     8581 accagattgg cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc
     8641 acgcgcgcca tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca
     8701 atcttcagct catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc
     8761 tgttgcatga gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcagt
     8821 aaatccgcct gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc
     8881 agccaggtat tcaggccgcg acgatcgccg gtgccaagcg ccattcgcac acggcgttca
     8941 ataagcgaag tagattgcga gcgcataatc gcgtcatcgc gccatttcgc ctgctcgtcg
     9001 gtgacatcgt tgcccatcaa acgccaggcg acaatatcgc gcagctcctg aatctgatct
     9061 tcattaagct gctgtgcctg agcaagcgac gggatcatca gccgtgcatt ttcagcatcc
     9121 tgccgcgcca cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg
     9181 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgaa
     9241 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccggtgtta
     9301 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
     9361 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
     9421 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
     9481 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
     9541 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
     9601 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca
     9661 tcagtgatct ggcggtattc caggtaggga taaagtgggt aatctttcag tccaggcatc
     9721 atttgttcga ccacatccat ttgtcgatta tcccaggcct gcttaatttg cgcgtaacgg
     9781 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
     9841 ccggcagcca acagccgcca ggtaacgtgt ttggcttttt ccacaagcgc atcctctaag
     9901 tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc
     9961 gtttacgcca cgttacggac acttttttac ttttacgggg tgatcgactg gctgggatta
    10021 gacagggaaa caggctacac ttcgcctttg aaaactcatc atcacgataa acatagaggc
    10081 gaagtccaac gtggctcaat tcgtttatac catgcatcgt gtcggcaaag ttgttccgcc
    10141 gaaacgtcat attttgaaaa acatctctct gagtttcttc cctggggcaa aaattggtgt
    10201 cctgggtctg aacggcgcgg gtaagtccac cctgctgcgc attatggcgg gcattgataa
    10261 agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag attggttatc tgccgcagga
    10321 accgcagctg aacccggaac acaccgttcg tgagtccatt gaagaagcgg tttctgaagt
    10381 ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg tatgccgatc cggatgccga
    10441 ttttgacaag ctggccgctg aacaaggccg tctggaagag atcattcagg ctcacgacgg
    10501 tcacaacctg aacgttcagc tggagcgtgc ggcggatgcg ctgcgtctgc cggactggga
    10561 tgcgaaaatc gctaaccttt ccggtggtga gcgtcgtcgc gtggcgttgt gccgcctgct
    10621 gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc aaccacctgg atgcggaatc
    10681 cgtagcgtgg ctggagcgct tcctgcacga cttcgaaggc accgtggtgg cgattaccca
    10741 cgaccgttac ttcctcgata acgttgcggg ctggatcctc gaacttgacc gcggtgaagg
    10801 tattccgtgg gaaggtaact actcctcctg gctggagcag aaagatcagc gtctggcgca
    10861 ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg agtgggtacg
    10921 tcagggaact aaaggccgtc agtcaaaagg taaagcacgt ctggcgcgct ttgaagaact
    10981 caacagcacc gaatatcaga aacgtaacga aaccaacgaa ctgtttattc cacctggacc
    11041 gcgtctgggc gataaagtgc tggaagtcag caacctgcgt aaatcctatg gtgatcgtct
    11101 gctgattgac tccctgagct tctcgatccc gaaaggagcg atcgtcggga tcatcggtcc
    11161 gaacggtgcg ggtaaatcga cgctgttccg tatgatctct ggtcaggaac agccggacag
    11221 cggcaccatc actttgggtg aaacagtgaa actggcgtcg gttgatcagt tccgtgactc
    11281 aatggataac agcaaaaccg tttgggaaga agtttccggc gggctggata tcatgaagat
    11341 cggcaacacc gagatgccga gccgcgccta cgttggccgc tttaacttta aaggggttga
    11401 tcagggtaaa cgcgttggcg aactctccgg cggtgagcgc ggtcgtctgc atctggcgaa
    11461 gctgctgcag gttggcggca acatgctgct gctcgacgaa ccaaccaacg acctggatat
    11521 cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc ccgggctgcg cgatggttat
    11581 ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac attctggatt accaggatga
    11641 aggtaaagtt gagttcttcg aaggtaactt taccgagtac gaagagtaca agaaacgcac
    11701 gctgggcgca gacgcgctgg agccgaagcg tatcaagtac aagcgtattg cgaagtaatg
    11761 cgtaaaatgc cggatgcggc gtaaacgcct tatccggcct acaaaccaag caatttcaat
    11821 acgttgtgaa tctccaggcc tgataagacg cggtaagcgt cgcatcaggc attggttctt
    11881 acctctgctc ccccatcatc tcccgcacca gctccacgca gcgcagaaaa cggctgtcgt
    11941 aatcgtcctc ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca
    12001 agttctggaa ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc
    12061 acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca atcaacgcct
    12121 gcacaaacgg atgctcacgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat
    12181 cggtgtcgat aaacgccact ttattggcat atttcactgc aaaatcaata tattgcgcat
    12241 gtcccagcgc gattttatcg tagtcggaat actgcaacgc gatctcatcg ccaccgaggt
    12301 gcgaaaagac ataatcacga ccatattccc acgcactggt agtgttgaag atattggcaa
    12361 gtttgtttac cagggtggat ttaccgctcg attcaccacc gaggatcgcc acggtacgca
    12421 caaagaatgg cttcacttcc gtaggaatat attcccagta gcggaacggg ttttcgcgga
    12481 tctgcgcacc gctgatactc ataaaggtac gcttcggatc gaccagcacc gtctcgatcc
    12541 ccagatgttc catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct
    12601 gaatcccttt ttcagccata aactttttga tgccgttgct ccatacatcc cagccgtgtg
    12661 gatatggctc catgccctct tcattgaaag catgaatacg gatatttttc tgatatttaa
    12721 aggtttgcaa tagccagcgc agacgatccg gcaccgtggg ctgctgcgac atggcgctgt
    12781 cttcgaacaa tgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa
    12841 cctggctaca ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg taaaacttac
    12901 cgaatacgac accgatcgtt ttcttctgcc ggggaaattc aagtcccaaa taacggtgca
    12961 atgcctccag cttttgtgcg ctggggcttt taattttggc attcagtaac tggcttaaat
    13021 accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga
    13081 tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc
    13141 gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg
    13201 cgtttttttc ggcacgttag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc
    13261 ttctgagatt cgttcctggc cgctgggcac cgggcggatc tcccctgcca gcccgacttc
    13321 accaaacacc accagatcct gcggtagcgg tctgtcacgc aggctggaaa ccatcgccag
    13381 cagtaacgct aaatcggcac tggtttccgt caccttcacg ccaccgacca cgttaacaaa
    13441 cacatcctga tcggccattt gtagaccacc gtgacggtga agcaccgcca gcaggattgc
    13501 cagacggttt tgctccagcc cgactgccac gcggcgtgga ttcgccatca tcgagtgatc
    13561 gacgagcgcc tgaatctcca ccagcagcgg acgcgttcct tcccacacta ccatcactga
    13621 gctaccggag gtcacttcat cgccgcgact taagaaaatt gccgaagggt tgctgacttc
    13681 acgcagcccc tgctcggtca ttgcaaagac acccagctca ttcaccgcgc cgaagcggtt
    13741 tttatggctg cgtaaggtgc gaaaacggga gtcggcatcg ccatccaaaa gcaccgaaca
    13801 gtcgatacag tgttccagca ctttagggcc agccagcgag ccatctttgg ttacgtgccc
    13861 aaccatgaca atcgccacac cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc
    13921 acgcacctgt gccacgctgc caggcgatga ttgtacatcc gccatatgca tcacctggat
    13981 ggagtcaatt accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat
    14041 gctggtttcc gacagcatat taagattgtc agtcggcagg ccaaggcgat gagcgcgcat
    14101 tgccacctgt tgcagcgact cttcgccggt gacatacagc gttttcatct gctgcgccag
    14161 tttgcacagc gtttgcagca acagcgtgga tttccccgca ccagggttac cgccaatcag
    14221 aatggcgctt cccggcacca cgccgccgcc taatacgcgg tcgaactctt taaatccggt
    14281 ggaaaaacgc ggcagctctt caaggctgat gtcggaaagt ttctggactt ttgccactcc
    14341 ggcgctaccg gcatagccac tgagacgctc gttacgcgcc accgttggcg acgctgcaag
    14401 acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct gccagcgcgg
    14461 ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacgatgta
    14521 cctcgttaat gctgtgccgc ccgcaggatg acgggcggac aattacttct gattcaggct
    14581 gccagagagg atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgtctt
    14641 ttcattcact tttggcttgg catggtaggc aatccccagc cctgccgctt tgatcatcgg
    14701 caggtcattg gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg
    14761 cgcgaggcgg gtcagggttt tcgctttata ttgcgcgtca acgatgtcgc cgatcacatt
    14821 gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag
    14881 cttgtcgcgc aggtattcag caaagaaagt aaagccgccg gaggcaatcg ccactttcca
    14941 gcccagcgtt tccagcttga gtaccagctg cgttaagcct gacatcagcg gcagattttc
    15001 acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag
    15061 gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg ccaccctctc
    15121 gcccgttccg gccagtttgg caatttcatc aatacattca atctggatgg cggtggaatc
    15181 catgtccatc accagcaaac ccggcgtgcg caggtgtggg attttcccca gcggtgcgac
    15241 atccagttgc gcttcatggg ccaggcgcgt agcccgcgca gtgagtgaac ctgccagacg
    15301 aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatagccg cgcccagttt
    15361 gctctggtat tgggtcagac gttgtttatc cagcccacga ccatacagca gccagccgct
    15421 acgacctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc
    15481 aggccataaa gagacatctt caggcaagtc gcaccaggta atgttaggca ttaaggctcc
    15541 tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct
    15601 ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc
    15661 cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc gctgatgcag
    15721 ggggcgtcat ggtttagtca aaaccaccag cgacagcgta atccgcaact ggaagaactg
    15781 gcccgcaccc tggcgcggca ggtgacgcta aacgttgcac cgctgatgcg taccgactca
    15841 ccggatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag ccgcattctc
    15901 gacgcaggcg tatatgatga acaaggcgat cttatcgccc gttctggcga aagcgtcgac
    15961 gtgcgcgacc ggctggcgct cgacggcaaa aaagcgggcg gctattttaa tcagcagatt
    16021 gtcgaaccca ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac gctcgacacc
    16081 catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg
    16141 ttgctgctct cgctggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc
    16201 acccgctggc agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaggaa
    16261 gaaagcgaga aaaaagagtg acccattact acaagaaagg aaatcgttat gtccacatta
    16321 cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtaga ggagtgtatt
    16381 tttcgccaga tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta
    16441 gtgattggtc gtgcgcagaa cccgtggaaa gagtgtaata cccggcggat ggaagaagat
    16501 aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga tctcggcaat
    16561 acctgtttta cctttatggc tggcaagccg gagtacgata aaactatctc cacgtcgatt
    16621 gtgctcaatg cgctgaacgc gcttggcgtc agcgccgaag cttccgggcg taacgatctg
    16681 gtggtgaaaa ccgccgaagg cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa
    16741 gatcgtggct tccaccacgg cactttgctg ctcaatgccg accttagccg cctggcaaac
    16801 tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacctcagt gcgttcccgc
    16861 gtgaccaatc tgaccgaatt gctaccgggg atcacccatg agcaggtttg cgaggccata
    16921 accgaggcct ttttcgccca ttatggcgag cgcgtggaag cggaaatcat ctccccggac
    16981 aaaacgccag acttgccaaa cttcgccgaa acctttgccc gccagagtag ctgggaatgg
    17041 aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttac ctggggcggc
    17101 gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac
    17161 agtctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg tctgtaccgc
    17221 gcggatatgc tgcaacagga gtgcgaagcg ctgctggttg acttccagga acaggaaaaa
    17281 gagctacggg agttgtcggc atggatggca ggggcggtgc ggtagttacc cacccatgcg
    17341 ggtaactttc tcttcgattt gccggatttt ttccggcatt tcttgcgcaa tgtggcgaaa
    17401 cgcctcgctg atacgcggat ctctggcgac cgtttgccag aactgctgcg ccagcgggat
    17461 cgccgttaac catgcgcttt tactgatgtt aagatcaaac ttcacctcaa tggcagcatc
    17521 acgcagcatt ccggcgctgt ttggcgcata ggccatcggc agcatgtcgt agacgggtgt
    17581 cagcgcaaat ggtggttcag ataaataaaa tgataaattg cctgcgtgca tatcgctgtt
    17641 ggcgataagt cgcccaaacg cccagatcac ttctgtttgc gccacgctct ggtgagtgac
    17701 aagttgttgc tcacacaaat gccgcattgc ctccggccac gatcccggag aagagataaa
    17761 ctcactttgc accgcctcca gcgacacaat gggcaagcga ccatcgttac ctttgcagtc
    17821 aaagcgttcc gcttccagga atacctgcct gttacttgtt acaagcaccg ttgactcgat
    17881 ggcgtggatc ccgccgtcac gcaggatttg cgcggcaata gattcagcaa ttagcaggtc
    17941 cccccaacgt tggctgactg cggtttgctg tggcacggtg aatttcacca acacatgttt
    18001 attgcctgac ggcgtttgtg catagcaggt aaattttggc tgctcgccgc ccgcagaaga
    18061 acccacaatt tctccagcca gagcatcgct tgccaactgt tcgtaacggg tgagtttttg
    18121 atccaaagga atttccgcag ggcgttgtgc agtaatccat cgctgataat tcccctcccc
    18181 gaccaaccaa ccgccagtat attcaccgtt aaatacggtc agggcgtaga gcacatcttc
    18241 ttcctgccag agacgtatat catcagtcag attcagttgc gcggctaact ttctgcccca
    18301 cgcgcgccct aaaaagccct gcggtcggag atcggtcaaa taccagggca aaccatcaaa
    18361 ccactgttcg tcgccatctg cgcctgttac cagacaactt ccctgtggcc agcacaaccg
    18421 gatgtcggcg aatttatgcg cctttccggc atcgtccacc cgccagacgg gaatacgctc
    18481 aattccgcga taaggacgca gcagtacata tcgcgttgcc cgtgctttac caaagcgaat
    18541 cacccgattt tctctggcaa caaggcgtga gaacgtcgcc tgactgattg ccagacgctg
    18601 gcgcaattcc ggggcagaac gcggcccttg cagtaaaaga tcggtcagtt cgctcattaa
    18661 tgaatagatt cgtgaataga tacttgaata gatttattaa catatttcac tgaaaagtat
    18721 agcgaaaata ttcaagctgg agagataaat gaataaatac taaaaagccg gagcagtctc
    18781 ccactccggc ttcacacgac aatcgccttt cagcaaaaca caattactct ttatcgccca
    18841 gcagaacgga ttccagcgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    18901 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    18961 cgccaaattc tgcagcgacg ccgtagatac ccgccgcttc catttccacg ccgaggatgc
    19021 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    19081 acaggttacc aacgcgagca tcaacgccca gtgcttttgc cgcgtctacc gcgttacgca
    19141 ccatgtcgaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    19201 tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    19261 cagcgccaca ggaacccacg cggataattt tcttcacgcc gaaatcagtg atcagttctt
    19321 tggtgtagat ggagcaggac gggataccca taccgtggcc cattacggaa attttgcggc
    19381 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    19441 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    19501 cagcaaaatc gcccatttct gcattaatgt gtggagtagc catcgtttta tcctttttgt
    19561 gacatattaa aggcggagtc actgctccgc ccaaatccat cagaacatgg ctttgccata
    19621 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    19681 acgatgaccc agcgaacctg gtttcacttt cgggccgtaa accagtaccg gaatgtgttc
    19741 acgcgtgtgg tcagtaccgg tccaggtcgg gtcacaaccg tggtcagcag tgagaatcag
    19801 aatatcgtca tcgcgcagca aagacatcag ctccggcaga cggcggtcga acagttccag
    19861 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    19921 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    19981 cagcgcgtcc agcccggtcg ctttcacttt tttggtgata ccgcagttgg catagatgtc
    20041 tgcaatttta ccgaccgaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    20101 ggtcggtgct ggcggctcaa cagccaggtc gtgacggtta ccagtacgct ggaagttacc
    20161 ggctttgtcg ccgataaacg gacgagcgat aacacgaccg atgttgtagc cgccgttggt
    20221 cagctcttca cgggcgattt cgcacagctc gtagagttta tccaggccaa aggtttcttc
    20281 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    20341 catatgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    20401 gtaacccggc agattagcgc gttcgaccag tttatccagc aactcttgcg ggaagctgtt
    20461 ttcgtgatcg gagaagtatc cccactcaaa cagaaccggc acaccagcaa tttcccagtg
    20521 accagacggg gtgtctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    20581 ttcagcgttg ccatccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    20641 cagcccaaga cgggtcagat ttggcagatt gagcgggcct ttacgaccgt ggtcagcttc
    20701 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    20761 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    20821 catatgttct ccgtactcag tgcttcaaat ttaaatgatc ggatcagtat accgttattc
    20881 gctgatgcgg cgatagacag ttggtgtgct ttccggtgct ttatcggcaa gtttaattgc
    20941 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcgt ggataactgc
    21001 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    21061 gtaatcgatg gtgtcagatg cctgacggcg tccgccgccc attgcaacca ctgccatccc
    21121 cagtgcgcgg gtatccattt cactgacaaa cccttcggta tcagcataga ctgctttcgt
    21181 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    21241 tgccgcgacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    21301 tttcgcgcgc gcttcggcgt catttttcgc cagtttgccg gagatcagca tctccacgca
    21361 cagtgccatc gtgacatcaa acagacgcgg gttacgatgt tcacccgtca ggaactgcac
    21421 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatatcggt
    21481 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    21541 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    21601 cagcgcgtcc agaccttccg ccagtttctt cgccagaata gaggcggtga tcagcgggat
    21661 tgaatccacg gttgcggtaa tatcgcgggt cgcgtagaaa cgtttatccg ccggagccag
    21721 tgagctggtc tggccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    21781 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    21841 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccacaggcag cgaccattgg
    21901 ccccaacatc agcgaagtca catcgccgac accgccggtc gagtgtttat caacaatcgg
    21961 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    22021 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    22081 aatctgccct tcggagatag tgttgtcgcg aatgccgttg ataaagaaac gaatttcttc
    22141 atcactcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    22201 aacctcccgt ggaaaagaga cgaggtaatc ggggaaccgg atgagcatcg ctgcatcatc
    22261 cggcaaatca agccttagta gctgctggcg ctcttaccgt cgccgtggcc cagagctttc
    22321 agcaggcttg ccagcaagct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    22381 gcaccgaaca gttcatctgc aatggcgaga tatttctgcg catcttccgc agtacgcacg
    22441 ccgcctgccg gtttgaaacc aacggttttt tctacgccca tatcgcggat cacttccatc
    22501 atgatgcgcg cgctttccgg cgtcgcattc acagccactt taccggtaga ggttttgatg
    22561 aagtcagcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    22621 agttcgccgg tttcaataat cactttcagc agtacgttcg ccgcagcgca agcctcttta
    22681 caggctttca ccaggtcaaa accaacctgc tcgttacctg ccatcagcgc gcggtacggg
    22741 aacaccacgt caacttcatc ggcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    22801 atttcgatgt cgtcgttacc gtgtgggaag ttagttaccg tagcgatacg gatttccggg
    22861 gtgccctgct ctttcagagt tttgcgagca atcgggataa agcgaggata gatacagata
    22921 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gtgcgatcac tttctcatcg
    22981 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    23041 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    23101 ttagtattct aacatctact ccgcaacaca cttcgatgta catcacaatt aaggaaatct
    23161 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    23221 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    23281 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    23341 acaacgttgc cagcaaattg tcaccagccc ggtacttagc ccggagcaga agcgccattt
    23401 tctggcactg gaagcagaaa acaatctgcc ttacccacag ctgcctgccg aagcccgccg
    23461 cgcgctggat gagggggtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    23521 ctatgtctta cccgattacg ctcgttttct ggcgcacggt tccgaatggc tggagctgga
    23581 aggcgcgaaa gaccttgatg acgcactctc tctgctgacc attctttacc accatgtacc
    23641 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    23701 tagaattcta acacaagacg agatcgatat tcgaataaaa cgtttctggc gttacctcga
    23761 cagaaccctg ccagacgcct ttatgcatgc caatatcggt ccgtctgatt cgcccattac
    23821 ccgtgcaata ttacgtgcag acgcagagct gaagcaggtt tcaccgaacc tgacctttat
    23881 ctacgatcct gatatcaccc ctgatgacct gctgctggaa gtggcgaaaa acgtctgtga
    23941 atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg
    24001 ctacgggatt gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt
    24061 acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg ctggatgact tctttacgcg
    24121 cactctaccg cactactgcc aacagcagat cgccatcatc gatgcgcggt gtgaattcct
    24181 ctatcaacaa tcacacttct ttgagaatag cttcctggtg aaagaagggt tgattgatcc
    24241 tgaacgtttt gtgccgatgt ttggcatgta tgggctggcg gaagcggtta acttgctgtg
    24301 tgaaaaagaa gggattgccg cacgctacgg taaagaggcc gccgcaaatg aagtgggtta
    24361 tcgcattagc gcgcaactgg cggagtttgt cgccaatacc cccgtgaaat atggctggca
    24421 aaaacgcgcc atgttacacg cacagtcggg gatcagttcc gatatcggca ccacgccggg
    24481 cgcgcgtttg ccatatggtg atgagccaga tccgattacc catctgcaaa ctgtcgcccc
    24541 gcatcatgct tattattatt ccggcatcag cgacattctg acgctcgacg aaaccatcaa
    24601 acgtaatccg caggcgctgg tacagctttg cctcggtgcc tttaaagccg gaatgcgtga
    24661 atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt accggttata tggtgcgttt
    24721 gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc aacaccacct ggctgggaga
    24781 agaagccgca cgcaacactc gtattctgga acgccagccg cgcgtgataa gccatgaaca
    24841 gcagatgcgc tttagtcagt aagattatcc ccttctcctg cgttgacggg ccaggcagtc
    24901 gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga
    24961 cgatgggacg ttgcaatgac tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc
    25021 agattgttga cggcaaagtg gtgtggaacg ctgtggtttg cgagcagtgt gatacctgcc
    25081 tgaagaggtg tccgcaacat gccacgccaa tggcgcaatc catgagcgtg gacgaagtgc
    25141 ttagccatgt ccgcaaagcg gtgctgttta ttgaagggat aacggtaagc agcggcgaag
    25201 ccacgaccca actgccgttt gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac
    25261 tgcgccacct cacctgcctg gtggacagta acggtatgtt gagcgaaacc ggctgggaaa
    25321 aattactccc ggtgtgcgac ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc
    25381 atcagcaatt aaccggacgc gataatcagc agattaagcg cagcatctgt ttgctggcag
    25441 agcgcggtaa gctggcggaa ctgcgtttgc tggtgatccc tggccaggtg gattatttgc
    25501 aacacatcga agaactggcg gcgtttatca agggactcgg cgatgttccg gtacgcctga
    25561 acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg
    25621 aagacgttga gccgttggct gatgcgttaa aggtgcgcgg ggtgagccgg ttgatatttc
    25681 cggcgctcta tttgtgacat gccggatacg gcctacggtt cggcacagac ttgtaggcat
    25741 gataagacgc gacaagcgtc gcatcaggca ttatgcgccg actgccggat gcggcgtgaa
    25801 cgccttatcc ggcctacggt tcggcacaga cttgtaggca tgataagacg cgtcagcgtc
    25861 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    25921 tcggcacaga cttgtaggca tgataagacg cgtcaagcgt cgcatcaggc attgtgcgcc
    25981 aactgccgga tgcggcgtga acgccttatc cggcctacgg ttcggcacaa acctgtaggc
    26041 atgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg
    26101 aacgccttat ccggcctacg gttcggcaca aacctgtagg catgataaga cgcgacaagc
    26161 gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggta aactacccac
    26221 taacgctaaa caacgcatac gtattattaa gcaacacttc ggcaatctca tcttctggtt
    26281 ccgggcgcaa ctcgcaaagc acgtcaaaca cccgcacagc ctgttccggt cggttaggct
    26341 gcccctgaaa gccgttgagc ggcatatccg gcgcgtcggt ttccagcaac aacgatgcca
    26401 gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg atagtaccgc
    26461 ctacgccgat tttgtagccc agctgtacaa accgctcggc ctgttgcagg ctgccggaaa
    26521 aaccgtgaac cacgccagtg cacggtaaat cgtgacgttt aagatgcatc gccagtttgt
    26581 cgtgtgtacg ccgggaatga aggatcaccg gcagatcgta gcgtttcgcc agtttcagct
    26641 gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag agatccagac
    26701 cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc tgtagctgat
    26761 caagagacac atcgctatgt ttttccaaca tccccggatg caaccccaat gcggcataca
    26821 gcggttgata tttttccgct aacgcctgca cgcgggcaaa attttccgcc tctgtcgccg
    26881 gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc tcttcatcgc
    26941 cactaaacgg cgggaaatca aaatggcagt gggtatcgat aaaacggcaa atcaagccag
    27001 atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagca tcgctcactt cggcaaccag
    27061 cgtatcgttg gcgactggcg caggcggaat aacgatcgat tccggcgtca ccactggcac
    27121 cagatggcag gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg cgaggaaata
    27181 acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc gactgccaag
    27241 cgcgatacta tgtaatggct tcggtggata aatttcgaat atccgcagct tgcccggtgg
    27301 tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca ccagattgac
    27361 cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc actgcggcgt
    27421 gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc cctgccttgc
    27481 cgcctcttta accggaatcg catcactgat cccgccatcc aggtagttaa tgccttccag
    27541 cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca catccagcca
    27601 gttttgtttg gttggtaaaa agtaattagg cgcgtagtca tcctgacgac aggcgcacat
    27661 ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca acggcatctg
    27721 gcttgcggtg gcctccacca gccagtcgag atcgataaga tttcctccac ggacaaagcg
    27781 caatggatcg aaaaattcgc gttttgtggt atagcgcatg atgactttgc gcgcgtaacc
    27841 gggctgattg cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc cgagataaag
    27901 atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc acgccagccg tgaaaattcc
    27961 acgctgtccg ccgccttcgc acaccagagc tattcgtcca ggctggaacg gccttagcga
    28021 caacggcgca atattaccaa gcgttacagg tattcgctgc cccacctctg ccttcctgtt
    28081 tttgttgtga aagaatgaca aagtaacgca atttattatt cgctaaaacc gcaaaagcca
    28141 gtccgctgga ctggctttgg ataaaatttt cttaatgatc tgaaatctag ggtcgttttc
    28201 ggcccatgaa caaactcacc aggaacagaa taatcccgac gacaaagaca attttagctg
    28261 cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt aacgcgataa
    28321 ccagaaatat gatgccccaa cgaaacataa gcgtctcctt taccatagtt aatgtcaccg
    28381 cttgatatga gcgtgaaaat cactcaatgc gatattttta gtgtggtgca catcgcgcct
    28441 cccgacaaag ttcgggagga cgaattacga cgaattactt agttttcaga tcatttttaa
    28501 cgcttttcac accatctacc gctttggcga tactttcagc acggtcgctc tgtgcctggg
    28561 aatcgacggt accggagagc tgaaccacgc cgtcggtggt ttcaactttc acatgacggg
    28621 aagggacgat atcgtccgcc agcagtttgg ctttgatttc actggtggtg gcggtgtcac
    28681 ccgcgtagcc cttcacagag ccttctttag cgtcgcgaac gtgcagtttg tcgctgacag
    28741 aggtcacccc ttcaacgcct ttcgccactt tcactgcctc ttcggcctga gcctggcttt
    28801 caacgaaacc gctcagggtc acgacttttt gatcggtttt gacagagata tcggtgctct
    28861 tgatgttgtc atgatccacc agggccgcct tcactttcgc ggtgatggcg ctgtcatcca
    28921 tgaaattacc gactttattc atagagctat cgactttttg ccctgcgctt tcattggtag
    28981 tctgcgcgtt gttttccgcg taggcagagc ctgtcgcgac ggcagaggtc aacattacag
    29041 ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat cgatttattc ctgtaagttt
    29101 gctcgtaatt tgagcctggc aacacgaggt tgcattgctg aatagggaga gacttcaccc
    29161 tctacagaag tcaatggtcg ccatcacaaa agcgatgggt gatgaataac gaccattaca
    29221 gcctctgaat cagttattaa tatcggtaga atgacaatct acggctttag atactgatat
    29281 ctacgcattg aacggtattt aacgccgtca gaaatgtcat tactttgtta aatatagatc
    29341 acaattttga aaccgctcgg aatatcatcg aaaacataag caaaagtgaa tgttttaaga
    29401 acattccgta agcggctaat aaggaaggga aattgacagg gcgcagcggc taccgcgccc
    29461 tggaggcaag aattaatgct cgcgggtctg gtggaactga acgtccggat aacgttcctg
    29521 cgccaggcgc aggttgacca tgctggtagc gatgtaagcg aggttatcgc cgccatcaag
    29581 cgccagttgg ctttcgttct tacgcttgaa ctcttcgaat ttcttcgcgt ctgcacattc
    29641 tacccagcgg gcggtggcaa cgttgactga ttcatatact gcttcaacgt tgtattcgct
    29701 cttcaggcgc gataccacca catcaaactg cagcacacca accgcaccaa cgatcagatc
    29761 gttgttggag attggacgga acacctgcac cgcgccctct tcggaaagct gtaccagccc
    29821 tttgagcagc tgtttttgct tcagcggatc tttcaggcgg atgcgacgga acagttctgg
    29881 cgcgaagttc ggaataccgg tgaacttcat catctcaccc tgggtaaagg tgtcgccaat
    29941 ctgaatggtg ccgtggttgt gcaggccgag gatatcgccc ggatatgctt cttcaacgtg
    30001 cgaacggtca cctgccataa aggtcagcgc gtcggagatc accacatctt tcgcagtgcg
    30061 cacctggcgc agcttcatgc ctttttcata tttaccggat accacgcgca taaacgccac
    30121 gcggtcgcgg tgtttcgggt ccatgttggc ctgaatttta aatacgaagc cggtaaattt
    30181 gtcttcgctc gcctctacgg tacgggtatc agtctgacgc ggcatcggcg caggagccca
    30241 ctccaccagg ccatccagca tatgatcgac accgaagtta cccagcgcag taccgaagaa
    30301 taccggagtg atttcgcccg caaggaacag ctctttgtcg aactcattag acgcgccttt
    30361 caccagttcc agttcgtcac gcagctgctg tgccagatct tcaccgaccg ctgcatcgag
    30421 atccgggtta ttcagccctt taacaatgcg gacttcctga atggtgtggc ctttaccgct
    30481 ctgatagaga taggtttcgt ctttataaag gtggtaaacg cctttaaaca gcttgccgca
    30541 gccgatcggc caggtgatcg gtgcgcagcc gattttcagc tcgttctcaa cttcatcgag
    30601 cagttccatc gggtcgcgga tatcacggtc aagtttgttc ataaaggtga ggatcggcgt
    30661 gtcgcgcaga cgggtaactt ccatcagctt gcgggtacga tcttcaacac cttttgcggc
    30721 gtcgataacc atcagacagc agtccaccgc cgtcagggta cgataggtat cttccgagaa
    30781 gtcttcgtgc cccggggtgt cgagcaggtt aaccaggcaa tcgtgatacg gaaactgcat
    30841 cacagacgta gtaatggaga tcccacgctg cttttccatc tccatccagt ccgacttagc
    30901 gtgctggttg gaaccacggc cttttactgt accggcggtc tgaatggcct gtccgaacag
    30961 cagcaccttc tcggtgatgg tagtcttacc ggcgtccggg tgagaaataa tggcaaaagt
    31021 gcggcgcttc gccacctctt gcaaataagg agacaacgtc ataattcttt cttcttgagt
    31081 aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta cccatcaacg
    31141 ggggggaggc aatcagtgtt tacacaggag ctgctccagt tcgtgcaacg aagaaacggt
    31201 ccaggtgggc gcgatgcctt ctggttgctc gcgatggtgc gcattcagcc agcaggtcgc
    31261 aagcccggcg ttgatgccac cgagaatatc ggactcggca gtgtcgccaa ccatcagcac
    31321 gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg
    31381 tttggcaacg ccaacttctt cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt
    31441 gcgttccaga cgcacttgct gcaaggcgct aaagccgttg gtgatgatgc cgattttagc
    31501 gttgccacga atggcgttaa gcagggaaac cgcccccggc aacggcgtac agatttccgc
    31561 catcgcatta ataaaggcct cgttgagttt acccggttcg acgttcagcc gttcggccca
    31621 gctctcaaac cgcccgtgct gaagctgtaa tgaagtgatc acgccgtttt gataatccac
    31681 ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct
    31741 gtaatcaaga aacatccgct gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc
    31801 ggcatcaaag aaaatccagt cccacttcat tattccacct tgtcttacat actgattggt
    31861 aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc
    31921 gtcgcctcgt taaagcctaa actttcgtac agggcaatgg cggcagcatt tgaggcacgg
    31981 acttccagcc atagcgtcgc cacgccgcgt ttttccagtt cgtcgatcag atgttccagc
    32041 agcgcccttc ccagtccctg acgctgatag tcaggatcga ccgcaatatt gaacaatgta
    32101 gcttcatcca gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac
    32161 tgaaagttga gatagcgctc gccctggttg ctggcaaagg ttttttcact ccacggaaag
    32221 gtgtgggcgc gttgttcaat gtggaaagcc gctggtaaat cagtcgtttc gagggaagaa
    32281 atcgtgttca tatgtgcaaa tttgttgcca taacgcggcg cgtgccgttg ggtttgcccg
    32341 taattcggtg agcgccggtg atgtcacctg agcgccttcc agtgatagcg gttcgtcagt
    32401 acccaaccgc cagctgttgc agcggctgcc ttgcggcagc atcgcgattt tttctggcgt
    32461 cagttgcagc acttgatcgg ggctgacggt taacgcacgc agaacatcgc tcaccagagg
    32521 atcggtcagg gcgggaagat cgtttgccac catcaccaga cggacgtgtg gcgggatcgc
    32581 aatggcaatc tccccctgca acgcgccagg gcgacgcagc gaccactggg taatgcccag
    32641 ttgctgtaac tgccagtctc gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg
    32701 caaatatagc aaattcgtcg ataccgcgcc aacatatggc tataatcgcc gccagtatca
    32761 attgaggagc attccatgtc tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt
    32821 gatgatttcg aacaaagccg tattctgttt gccggagact tacaggatga cctgcccgcg
    32881 cgtttagata ccgcggccag ccgtgctcat acccagcaat tccaccactg gcaggtgtta
    32941 aaccgccaga tgggggataa cgcccgtttt agcctggtcg ccacggcgga tgatgtcgct
    33001 gattgcgata ctttgattta ctactggccg aagaataaac cggaagccca gttccagttg
    33061 atgaatttac tttctctgct tccggtgggc acggatattt ttgtcgttgg cgagaaccgc
    33121 agcggcgtgc gcagcgccga gcagatgctg gccgattatg cgccgttgaa taaggtagac
    33181 agcgcccgtc gctgcggcct ctattttggt cgtctggaaa agcagccggt atttgatgcc
    33241 aataaattct ggggcgaata cagcgtcgat ggcttgacgg tcaaaacgct gccgggcgtg
    33301 tttagccgcg acggtctgga tgtcggcagt cagttgttgc tctcgacgct aaccccgcac
    33361 acgaaaggta aagtgctgga tgtcggctgc ggcgcaggcg ttctttccgt tgcttttgcg
    33421 cgccattcac cgaagatccg cctcactctg tgtgatgttt ctgcaccagc ggtagaagcc
    33481 agtcgcgcaa cacttgctgc caacagtgtt gaaggtgaag tctttgccag caacgtcttt
    33541 tccgaggtga aaggtcgttt tgatatgatc atctccaacc cgccgttcca cgatgggatg
    33601 caaaccagcc tggatgcggc gcaaacgctg attcgcggcg cggtgcgtca tcttaatagc
    33661 ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt gctggatgag
    33721 acatttggtt tccacgaagt gattgcgcaa accgggcgct tcaaggtgta tcgcgccatt
    33781 atgacccgcc aggcgaagaa aggttaagta tctgatatct gtcgccgcat accgtgttgg
    33841 atgcggcgta cacgccttat ctgaccgaca aacgaattat ccacaatccg ctgatggaca
    33901 tttttccagc aattacacct ctgtcgataa ttaactattg acgaaaagct gaaaaccact
    33961 agaatgcgcc tccgtggtag caattctttt taagaattga tggtatgcga aggtggcgga
    34021 attggtagac gcgctagctt caggtgttag tgtccttacg gacgtggggg ttcaagtccc
    34081 ccccctcgca ccaaaaacca cgttgatatt gctcgc
//
LOCUS       DAPQZD010000037        34023 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPQZD010000037 DAPQZD010000000
VERSION     DAPQZD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.037"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(59..754)
                     /gene="aqpZ"
                     /locus_tag="RO454_001719"
     CDS             complement(59..754)
                     /gene="aqpZ"
                     /locus_tag="RO454_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDY0933565.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASVFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1179..2837
                     /locus_tag="RO454_001720"
     CDS             1179..2837
                     /locus_tag="RO454_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDY0933566.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2834..3790)
                     /locus_tag="RO454_001721"
     CDS             complement(2834..3790)
                     /locus_tag="RO454_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDY0933567.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            3941..5056
                     /gene="macA"
                     /locus_tag="RO454_001722"
     CDS             3941..5056
                     /gene="macA"
                     /locus_tag="RO454_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDY0933568.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5053..6999
                     /gene="macB"
                     /locus_tag="RO454_001723"
     CDS             5053..6999
                     /gene="macB"
                     /locus_tag="RO454_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDY0933569.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7072..7296)
                     /gene="cspD"
                     /locus_tag="RO454_001724"
     CDS             complement(7072..7296)
                     /gene="cspD"
                     /locus_tag="RO454_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDY0933570.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7619..7939
                     /gene="clpS"
                     /locus_tag="RO454_001725"
     CDS             7619..7939
                     /gene="clpS"
                     /locus_tag="RO454_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDY0933571.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7970..10246
                     /gene="clpA"
                     /locus_tag="RO454_001726"
     CDS             7970..10246
                     /gene="clpA"
                     /locus_tag="RO454_001726"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDY0933572.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10590..10677)
                     /locus_tag="RO454_001727"
     tRNA            complement(10590..10677)
                     /locus_tag="RO454_001727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10641..10643),aa:Ser,seq:gga)
     gene            complement(10931..11149)
                     /gene="infA"
                     /locus_tag="RO454_001728"
     CDS             complement(10931..11149)
                     /gene="infA"
                     /locus_tag="RO454_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDY0933573.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11434..12138)
                     /gene="aat"
                     /locus_tag="RO454_001729"
     CDS             complement(11434..12138)
                     /gene="aat"
                     /locus_tag="RO454_001729"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDY0933574.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(12180..13901)
                     /gene="cydC"
                     /locus_tag="RO454_001730"
     CDS             complement(12180..13901)
                     /gene="cydC"
                     /locus_tag="RO454_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDY0933575.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13902..15668)
                     /gene="cydD"
                     /locus_tag="RO454_001731"
     CDS             complement(13902..15668)
                     /gene="cydD"
                     /locus_tag="RO454_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDY0933576.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15791..16756)
                     /gene="trxB"
                     /locus_tag="RO454_001732"
     CDS             complement(15791..16756)
                     /gene="trxB"
                     /locus_tag="RO454_001732"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDY0933577.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17300..17794
                     /gene="lrp"
                     /locus_tag="RO454_001733"
     CDS             17300..17794
                     /gene="lrp"
                     /locus_tag="RO454_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDY0933578.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17929..22035
                     /gene="ftsK"
                     /locus_tag="RO454_001734"
     CDS             17929..22035
                     /gene="ftsK"
                     /locus_tag="RO454_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDY0933579.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22194..22805
                     /gene="lolA"
                     /locus_tag="RO454_001735"
     CDS             22194..22805
                     /gene="lolA"
                     /locus_tag="RO454_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDY0933580.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22816..24159
                     /gene="rarA"
                     /locus_tag="RO454_001736"
                     /gene_synonym="mgsA"
     CDS             22816..24159
                     /gene="rarA"
                     /locus_tag="RO454_001736"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDY0933581.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24250..25542
                     /gene="serS"
                     /locus_tag="RO454_001737"
     CDS             24250..25542
                     /gene="serS"
                     /locus_tag="RO454_001737"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDY0933582.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(25848..25988)
                     /locus_tag="RO454_001738"
     CDS             complement(25848..25988)
                     /locus_tag="RO454_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDY0933583.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(26180..26440)
                     /locus_tag="RO454_001739"
     CDS             complement(26180..26440)
                     /locus_tag="RO454_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDY0933584.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHDLPASHRGNNQMSLPLSQN"
     gene            complement(26481..27590)
                     /locus_tag="RO454_001740"
     CDS             complement(26481..27590)
                     /locus_tag="RO454_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDY0933585.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            27748..28932
                     /locus_tag="RO454_001741"
     CDS             27748..28932
                     /locus_tag="RO454_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDY0933586.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGRTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            28932..29444
                     /locus_tag="RO454_001742"
     CDS             28932..29444
                     /locus_tag="RO454_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDY0933587.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            29500..29874
                     /locus_tag="RO454_001743"
     CDS             29500..29874
                     /locus_tag="RO454_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933588.1"
                     /translation="MKDIDTETRNNTVADDVTSGEDMAVERGVKLSRPIERGAEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            29883..30038
                     /locus_tag="RO454_001744"
     CDS             29883..30038
                     /locus_tag="RO454_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDY0933589.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            30025..32832
                     /locus_tag="RO454_001745"
     CDS             30025..32832
                     /locus_tag="RO454_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDY0933590.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     QARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRLLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            32845..33333
                     /locus_tag="RO454_001746"
     CDS             32845..33333
                     /locus_tag="RO454_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0933591.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(33362..33961)
                     /locus_tag="RO454_001747"
     CDS             complement(33362..33961)
                     /locus_tag="RO454_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0933592.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
BASE COUNT         8324 a         8459 c         9256 g         7984 t
ORIGIN      
        1 caacatataa caacatctta aaaaaaggcc tgacattgcg ccaggccttc tgcgttaatt
       61 aattacgctt ttccagtaga gtccggtaaa tcagaccacc gataataccg ccgacaattg
      121 gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg
      181 cagtactgcg cgccgggtta acggaagtgt tagtcaccgg aatactgatt aagtgaatca
      241 gggttaaggc cagaccaatg gcaattggcg caaaaccagc aggagcgaat ttgtcagttg
      301 cgccgtggat caccagcagg aaacctgcac tcaataccag ttcaactacc agcgcggaaa
      361 gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa acgctggctg
      421 ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgcc gcaacaatac
      481 cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccgggaaa cgtccgccag
      541 cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcaa
      601 aggccattgt cagaacggtc agaccgaacg ccaacgccac gccggcaaaa ccaatgccta
      661 attccgggaa gcctgcggcc agtacggcac taccacagcc gccaaaaaca agccagaaag
      721 taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct
      781 gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga aataatgcgc
      841 ggctatttta aaaacgaagg caaatcattc gccagataaa taaatccagt aaatttgatt
      901 tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagcg
      961 aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg
     1021 ttgtttttgc aggcttatca gcaagaggga gtataacgcg attattcgct catttttcag
     1081 acatttgcca tgcttaaatg tgatgccatc acgtattagc aggcctttcc cgttatactg
     1141 ccagcgtaaa gaataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa
     1201 ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga
     1261 ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc
     1321 cagaatcaga tctctaccat tttgagcgcg atgatttctg gttcccgccg ggagatatca
     1381 acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac
     1441 atcgggttcg ccgctatcgg ccgctggaag cgtgctggac gccatgcacc gatggttatc
     1501 accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc
     1561 gcagttttct cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggctc
     1621 gtcatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta
     1681 ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt
     1741 tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg
     1801 gactttccgc aatggtgcaa ctgcttgagc attatttttc tgagcaggga gctggacagg
     1861 cgcgatatcg tttaatgcgg cggcgagcca gcaatgagca acgaagctgg cgctatctgg
     1921 atatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg
     1981 gcctgtttgc tactttgttg caggcaaaag gcacattgcg actggataaa gacgcccgtc
     2041 cattgttgct gatcgaagat ccagaaaccc gtttgcaccc cattatgctt tcagttgcct
     2101 ggcatctgtt gaaccttctg ccattgcagc gcatcgctac taccaactcg ggagagttgc
     2161 tttcgttaac gccggtagag catgtttgcc gactggtacg tgagtcctct cgcgttgccg
     2221 cctggcgtct gggaccgagt ggcttgagta ccgaagatag ccgacgcatc tcctttcaca
     2281 ttcgttttaa ccgtccgtca tcgctgtttg cccgttgttg gctgctggtg gaaggggaaa
     2341 cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag
     2401 ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccggc
     2461 gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg
     2521 ctacggtacg cagcctgttg aataatgatc gggaagccga acgagaacat ttaacggcgt
     2581 taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc
     2641 gcgtggcgca aatcccggaa aatataccga tgaatctgcg caaaattatc tcgaaagcga
     2701 tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggcc ggacgtcgtg
     2761 gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc
     2821 gcggtcgggc cgactaacta caaaacatcg tggccatttg tggctgaata gcgtcgagca
     2881 tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc
     2941 gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt
     3001 cccagaaagc gttgtaatcg gcatggatct tgccttcttt atcgcgataa cgcaggctgc
     3061 ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa
     3121 acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg
     3181 cattctggat ttcctggtgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca
     3241 tcgtcctttt cccctgatat tcacacaggg taaaagtgag ctctgccaga ggaatacctt
     3301 cgctgttgcg gaacaggatg gtgctgtcac cttctttatc cattgagatc atcatggtca
     3361 gctcaagcgt gaactgctcg ccgtttttgc cttccagctt cgccagttgc agcccggggg
     3421 tattcaaata taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt
     3481 aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgta
     3541 gacgtacagg cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa
     3601 gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct
     3661 ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtcctcgt gccagactaa
     3721 gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg
     3781 acatattcat gaaatcaatg gttatacatg acgtcgattt aaccattgcg tatcttaacc
     3841 aaacatcaat agtgtgatta ttaacgtaaa ttttagggtt ttgttgatat ttcgttgaag
     3901 ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt
     3961 gaagaagcgt tacgttattg cgatggtgat agtcatcgcc gggctgatta cgttatggag
     4021 aattcttaac gcacccgtgc cgacttatca gacgctgatt gtgcgccccg gtgatttaca
     4081 gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca
     4141 ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca
     4201 gcttttaggg gttattgatc ctgaacaggc tgaaaaccag atcaaggagg tggaagcaac
     4261 gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt
     4321 gacgtattcc cgtcagcaac gtcttgcaca aacgcaagcg gtttcacagc aggatctcga
     4381 taacgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat
     4441 caagcgcaat caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgcatcgt
     4501 tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc
     4561 cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc
     4621 gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt
     4681 gcttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga
     4741 aaagattaac gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt
     4801 actgcggctg gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgct
     4861 gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt
     4921 gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga
     4981 tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc
     5041 aggagctgcg caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc
     5101 ggtgatgagc aggttgaggt gctgaagggc atcagcctcg atatttatgc cggtgagatg
     5161 gtagcgattg tcggtgcttc gggttctggt aaatcgaccc tgatgaatat tctcggctgc
     5221 ctggataagg ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc cacgctggac
     5281 gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat
     5341 ttgctttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt
     5401 gagtggaaac agcggctgct tcgcgcccag gagttactgc aacgactggg gctggaagac
     5461 cgtacagagt attacccggc acagctttcg ggtggccagc aacagcgcgt cagtatcgcg
     5521 cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac
     5581 agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg
     5641 gtgattatcg tcacacacga tccgcaggtt gctgctcagg ccgagcgggt gatcgaaatt
     5701 cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgc tactggcgga
     5761 acggaacctg ttgtcaacac ggcgtctggc tggcggcagt ttgtcagcgg ttttaacgag
     5821 gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg
     5881 ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg cgacgccgcc
     5941 aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga tgtctatccc
     6001 gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta
     6061 atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg
     6121 cgcctgcgtt ataacaatgt tgatgtggct gccagtgcca atggcgtgag cggcgattat
     6181 tttaatgtct atggcatgac tttcagtgaa gggaacacct ttaatcagga gcagctgaac
     6241 ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa
     6301 gcagatgtgg ttggcgaggt gattctggtt ggcaatatgc ccgccagagt cattggtgtg
     6361 gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctgccatac
     6421 agcacgatgt ccgggcgagt tatgggtcag tcgtggctga actccataac tgtcagggtg
     6481 aaagaaggct ttgacagcgc tgaggcggaa cagcaactca cgcgtttact ttcactgcgc
     6541 cacggtaaga aggatttctt tacctggaac atggatggcg tcttgaaaac cgttgaaaag
     6601 accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc
     6661 ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc
     6721 attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagtttct gatcgaagcc
     6781 gtactggttt gcctggttgg tggcgctttg ggaataacac tgtcactgtt aattgctttc
     6841 accttgcagc ttttcttacc cggctgggag attggttttt caccgttagc gctgctgctg
     6901 gcgtttctct gctcgacggt caccgggatt ttatttggct ggttacccgc acgaaatgcg
     6961 gcacgactgg atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag
     7021 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
     7081 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
     7141 gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt
     7201 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
     7261 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
     7321 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
     7381 ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg
     7441 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
     7501 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     7561 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     7621 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     7681 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattata ctccgatgga
     7741 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     7801 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ctgaggttgc
     7861 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     7921 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     7981 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     8041 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     8101 ggaagcgtgt tctgtagatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     8161 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgttgagttt
     8221 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac
     8281 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     8341 gcgcaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa
     8401 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     8461 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gagtgggcgg
     8521 aatcgaccca ctaattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     8581 tcgccgtaaa aacaacccgc tgctggtggg ggagtctggt gtcggtaaaa ccgcgattgc
     8641 ggaggggctt gcatggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
     8701 gatttactct ctcgatatcg gttccctgtt agcgggcaca aaatatcgcg gcgactttga
     8761 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
     8821 tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggccagg tcgatgcggc
     8881 taacctgatt aaaccgttgc tctccagcgg taaaatccgc gtaattggtt cgacaaccta
     8941 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     9001 tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     9061 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct
     9121 ggcggtgaaa tacattaacg atcgtcatct gccggataaa gcgattgatg ttatcgacga
     9181 agcgggcgct cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     9241 ggatattgaa tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag
     9301 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
     9361 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     9421 acataaaccg gtcggttcgt tcctgtttgc cggccctact ggggtcggga aaacagaggt
     9481 gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta
     9541 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     9601 tcagggcggt ttgctgactg atgcggtcat caagcatcca catgcggtgc tgctgctgga
     9661 cgaaatcgag aaagcgcacc cggacgtgtt caatattctg ttgcaggtga tggataacgg
     9721 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
     9781 cactaatgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa
     9841 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
     9901 cgacaacatt atctggttcg atcatctgtc aaccgacgtg atccatcagg tggtggataa
     9961 attcatcgtc gagttgcagg ttcagttgga tcagaaaggt gtttctctgg aagtgagcca
    10021 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat
    10081 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
    10141 gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta
    10201 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtctggt
    10261 aggttggtga agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggggagga
    10321 ggttcagacc ctttttttga tgatgatggt aagttattga taattagtgc tgcgggtagg
    10381 taaggataaa aaagggtggc agcaggagat tgagatggtt ttgctttatt aacaacgggc
    10441 taaacgtgta gtatttgagt tcactgccgt acaggcagct tagaaattgc cgcggatcct
    10501 gtctgccaat aatgacaagt tcactgccgt acaggcagat aaaatgcgaa aaaaaagccc
    10561 gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
    10621 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
    10681 ttgttttttt gcctgacctc atggggggca acggggcgct actataggga gttggaataa
    10741 aacggtcaag aagaattttt atgataatta ttgtttgctc atactgtaaa caagttgtgc
    10801 agtatatcta catcgagaca agttacggac ttatacttct aaagtactcc atacatatca
    10861 caaaataaaa aggccggcta aaccgacctt ttactcattc tttctcttcg cccatcaggc
    10921 ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca
    10981 gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg
    11041 agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca
    11101 acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg
    11161 gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa
    11221 gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta
    11281 agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga
    11341 cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt
    11401 ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcatcg
    11461 aggtacccag aaattattcg acaatcgtcc gaggcgcatt tgattgagat aattaaggta
    11521 atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca
    11581 gtcgataagt ttaccgccat gacggataaa ttcatcacag aagaccagaa gcgccgtttt
    11641 ggacgcattt tccatccggc tgaacatgga ctcgccacaa aatagtgttc cctgggccac
    11701 gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt gggcatgacc
    11761 gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc
    11821 gcgatcgctg gcacagcctt cgatgacctg accaaaagcg taattcatcg tgacacgata
    11881 gggtgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt ctggccataa
    11941 caccgcgcgg ggatcgggcg accaccagag gatggggtcg ccaggagaaa accacgggaa
    12001 aataccacgc tgataagcca ttaataggcg cgcagggcta agatcgcccc ccagtgccag
    12061 caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga
    12121 aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct
    12181 tacaaaccct gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta
    12241 ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg agagagcccg
    12301 cgaagtcgat gggtgaccat taacaccgtt ttctcgcaca tcatttctgc aagcaattca
    12361 aggatctgac tttcggttgt ggcatctaag ccttctgtag gttcatccag caataccagc
    12421 ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc
    12481 tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagcag cttctccaga
    12541 ccaacgcgac gcaagatctc cgccagagcc tcatcactac taccaggcga ggcgagtaac
    12601 agattatcac gcagcgtggc gctaaacaga tgcactcgct ggggaacaac gctgatggtc
    12661 tgtcgtagag ccgcttcatt caggctggct atgggactat cgttaagcaa aatctcgccc
    12721 tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg ttgacttgcc gcatccggtt
    12781 cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa gataaatccc tttaagtgcc
    12841 tgttgggatt gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg
    12901 gcaacacgag tttgggtatc agggaaggtg actttcggtt tttgatccgt taaatcagtg
    12961 atccgtacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc
    13021 agcgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga
    13081 gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca
    13141 atgagcagca ttatcgcttg cgacaatgcg gttagttcag attgacggcg ttgcgcttcc
    13201 agccattgaa tttctgtatt ctctaattgc gtgcgataac gatcgctggc accaaaaatg
    13261 gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg cccgcgaaga
    13321 tgagtcagat tttgcccggt gctttttccc gcacgataaa acagtggtgg catcaggaaa
    13381 agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac
    13441 ccgattgtca ccaccataat caccacaaaa gcgcctacca gcggcgagat aacgcgcagg
    13501 taaagatgat cgagcgtatc aacatccgcc accacgcggt tgagtaattc gccctgacga
    13561 tagcgcgcca gtcctgcagg ggagaggggc agtaatttgc tgaaggtgta aatgcgcaga
    13621 tgctgcaaca cgcggaaagt cgcatcgtga cttaccaggc gttcaaaata gcgcccggca
    13681 gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta gcatatagtt gaagctgtac
    13741 agtccggcaa cccccgcaac cgctgaggcg gagaggaacc agcccgaaag tgtcaacaga
    13801 ccgatactgg cgagcagcgt gacaattgcc agcacgatac caagacttaa catccattta
    13861 tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga
    13921 tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc
    13981 tcaataatct gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac
    14041 tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg
    14101 cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag
    14161 ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca
    14221 gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcactgacc
    14281 caggcgttat ccagcgctgt ttgtaactct tgttcgctgg catcaggtcg cgccagtagt
    14341 acgttatccc gtaatgttgc tgccggtaat tgtgggtttt gcccaaccca ggagagatgt
    14401 ttacgccatg attccggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc
    14461 tgatatgaga gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga
    14521 ccaaccaaca ctgctcgttg gcctgctggc aaagtaaaat tcagcggtcc ggccagcgtt
    14581 ttaccttccg gcgacgtgat aaacagatcc tcggcttcaa tggtcaccgg atcggtcgat
    14641 gctaattccg cttcaccgcg ctgcggatgg gctagcgggg tttccataaa cgttttcaaa
    14701 ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat
    14761 ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca
    14821 gtatcgtagt gaccaaaatc cagctcgccg aggtaggaaa aaccaaagta gaccgccacc
    14881 agagcaatcg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa cgccagccgt
    14941 agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca
    15001 gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc
    15061 ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca
    15121 accagcgcca taaataacgg aattagcggt gcagtgccca gcagaatgag cgccgcggcc
    15181 cagttagagg ggaagatagc caccacaatc agcagcggca ccgacactgc cagcgccatt
    15241 tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc
    15301 gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg
    15361 agaacctgac ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg
    15421 cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa acagtaacgt aaagggaagc
    15481 agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac
    15541 caggcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga aatattcagc
    15601 caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaattcttt ttgacgggat
    15661 ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac
    15721 ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttctttact tttgttactg
    15781 atttgtaaaa ttattttgcg tcagctaaac cgtcgaggta gcgttccgca tcaagagctg
    15841 ccatgcagcc tgtaccagca gatgtaatgg cctggcgata aatgtgatcc atcacgtcgc
    15901 ctgcggcaaa gacgccaggg atgctggtct gggtggcatt accatgaata cccgactgta
    15961 ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta ttcgggctgt
    16021 gaccgatagc aacaaacaga ccggcaacgt cgagtgactc gatattatcg gtgttttgcg
    16081 tatcgcgcag acgaacgcca gtgacgccca tctgatcgcc ggtcacttct tccagcgtac
    16141 ggttggtgtg cagaataatg ttgccgttct ccactttatc catcagacgc ttaatgagga
    16201 ttttttccgc acggaaacca tcacggcggt gaatcagatg aacttccgaa gcgatgttag
    16261 acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt
    16321 tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac tccacggcct ttaaaggctt
    16381 cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc
    16441 aagtgtattc gccgctatcg ccagtcagac ggaacggacg attctgcaaa tcaactttgt
    16501 tgatgtgatc aaagatgatt tcagtttcaa atttggtggc atgttcgtgc atgcgctcca
    16561 ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact tccgtggtgg
    16621 tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg
    16681 ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt
    16741 gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatggcagg attgtaggga
    16801 atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca
    16861 tcgataagca ggtcaaattc tcacgtcatt atcacctctg ctacttaaat ttcccgcttt
    16921 ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta
    16981 aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa
    17041 aaaaacagta ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag
    17101 tgaaatctac gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca
    17161 acggtcttct caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta
    17221 tcgtgacggg tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga
    17281 atacagagag acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg
    17341 tatcgatcgt aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct
    17401 ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag
    17461 acaagggttt attcagggct atacggcgct gctgaacccc cattatctgg atgcatcact
    17521 tctggtattc gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa
    17581 taccgctgta caaaaacttg aagaaattca ggagtgtcat ttagtatccg gtgatttcga
    17641 ctacctgttg aaaacacgcg tgccggatat gtctgcttac cgtaagttac tgggggaaac
    17701 cctgctgcgt ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa
    17761 gcagagtaat cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta
    17821 ttttgattac actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg
    17881 tccgttttag catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa
    17941 tacactgaag acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag
    18001 gcgttgctga tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt
    18061 aacccttcgg accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt
    18121 gggatgcccg gtgcgtggct ggcagatacg ctgttcttta tttttggcgt gatggcttac
    18181 accattcccg tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac
    18241 gaatacattg attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt
    18301 acctcctgtg gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc
    18361 gtcattggca gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact
    18421 attgcgctgc tctgcgtttg ggcagcgggc ctgacgctgt tcaccggttg gtcatgggtg
    18481 accattgctg aaaaactcgg cggctggatt ttaaatattc ttaccttcgc cagtaatcgt
    18541 acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa
    18601 gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta
    18661 gcgcgtcgta aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct
    18721 gcgttgttct ccggtaagcg gatggatgat gacgaagaga ttacctacac tgcacgcggt
    18781 gtggctgccg accctgacga cgtcctcttt tcgggcaatc gtgcaacgca gccagaatat
    18841 gacgagtacg atccattatt aaacggtgcg ccaattaccg aacctgttgc tgtagcagct
    18901 gctgctacca cggcgacaca aagctgggct gcaccggttg aacctgtgac tcagacgccg
    18961 ccagttgcct ctgttgatgt tccacctgcg caatctacag tagcctggca gcctgtaccg
    19021 gggccgcaaa cgggagaacc ggttattgct cctgcaccgg aaggttaccc acagcagcca
    19081 caatatgcgc agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccacag
    19141 cagccgtatt atgcacctgc agctgaacaa cctgcgcaac aaccttacta cgctcctgcg
    19201 gcggaacaac ctgttcagca accgtattac gcaactgcgg cggaacagcc tgcgcaacag
    19261 ccgtattacg cccctgcgcc agaacaagcg gtggcaggta acgcctggca agccgaagag
    19321 cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca
    19381 gccgctcagg aaccgttgta ccaacagccg caacctgttg aacagcagcc tgttgtggag
    19441 cctgaacccg ttgtagaaga gacaaaaccc acgcgtccgc cgctttacta ctttgaagaa
    19501 gtggaagaga agcgagcccg tgaacgcgaa caacttgcgg cctggtatca accgattcca
    19561 gaacctgtta aagaaccaga accgatcaaa tcttcgctaa aagcaccttc tgttgcagca
    19621 gtacctccag tggaagccgc tgccgccgtt tctccgctgg catctggcgt gaaaaaagcg
    19681 acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc
    19741 ggtggaccgc gtccgcaggt caaagagggg attggcccgc agttgccacg accgaaacgc
    19801 atccgcgtgc caactcgtcg tgaactggcg tcttacggta ttaagctgcc ctcacagcgt
    19861 gcggcagaag aaaaagcccg tgaagcccag cgtaatcagt acgattctgg cgatcagtac
    19921 aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagacg
    19981 cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtgaacac agaagatgca
    20041 gatgctgcgg cagaggcaga actggctcgt cagtttgctc aaactcaaca acaacgttat
    20101 tccggcgaac aaccggctgg ggcgaatccg ttctcgctgg atgattttga attttcgcca
    20161 atgaaagcgt tgctggatga tggtccacac gaaccgctgt ttacgccaat tgttgaacct
    20221 gtacagcagc cgcaacaacc ggtagcaccg cagcagcaat atcagcagcc gcaacaaccg
    20281 gttgcaccgc agccgcagta tcagcagccg caacaaccgg ttgcaccgca gcagcaatat
    20341 cagcagccac aacaaccggt tgcacagcag ccgcagtatc agcagccaca acagccggtt
    20401 acacagcagc cgcagtatca gcagccgcag cagccggttg taccacagcc gcaatatcag
    20461 cagccgcaac agccagttgc gccacagccg caggataccc tgcttcatcc gctgttgatg
    20521 cgtaatggcg acagccgtcc gttgcataaa ccgacgacgc cactgccttc tctggatttg
    20581 ctgacaccgc cgccgagcga agtggagccg gtagatacct ttgcgcttga acaaatggcg
    20641 cgcctggtag aagcgcgtct ggctgatttc cgtattaaag ccgatgttgt caattactct
    20701 ccggggccag ttatcactcg ctttgaattg aacctggcac cgggcgtaaa agcggcgcgc
    20761 atttctaact tgtcacgtga ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt
    20821 gaagttattc ctggcaaacc ctatgtaggt ctggagttac cgaataaaaa acgacaaacc
    20881 gtttatctgc gcgaggtttt ggataacgcc aaattccgcg ataatccgtc gccattaacc
    20941 gtggtgctgg gtaaagatat cgccggtgag ccggtggttg ccgatctggc gaaaatgccg
    21001 cacttgttgg ttgcggggac taccggttcc ggtaagtctg tcggggtgaa cgcgatgatc
    21061 ctgagcatgc tttataaagc acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa
    21121 atgctggagc tttcggttta tgaaggcatt ccgcacctgt taacggaagt tgttactgat
    21181 atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg tcgttataaa
    21241 ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc
    21301 gatcgcatga tgcgtccgat tccagacccg tactggaagc cgggtgatag tatggatgcg
    21361 cagcatccgg tgctgaaaaa agaaccgtac atagtggtgt tggtggacga atttgccgac
    21421 ctgatgatga cggtaggtaa aaaagtggaa gagctgatag cacgtctggc gcaaaaagct
    21481 cgtgccgcgg gtatccacct cgtactggca acgcagcgtc catcggttga tgttattact
    21541 ggtctgatta aagcgaatat tccgacccgt atcgccttta ccgtatccag taagattgac
    21601 tcacgtacca ttcttgatca ggctggtgcg gaatcactgc tgggtatggg ggatatgctc
    21661 tactctgggc cgaactccac tttgccggta cgtgtccatg gtgcttttgt tcgcgatcaa
    21721 gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta tgttgatggc
    21781 atcacctccg acagcgaaag cgaaggtggc gcgggcggtt tcgatggcgc tgaagagctg
    21841 gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc atcaatttct
    21901 ggcgtacagc gtcagttccg cattggttat aaccgtgcag cgcgtattat cgaacagatg
    21961 gaagcgcagg ggattgtcag cgaacagggg cacaacggta atcgtgaagt gctggcccca
    22021 ccgccgtttg actaattaat gcatcgtagg ccggataagg cgcggtagcg tcgcatccgg
    22081 caatctggta actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
    22141 atagagagta gagggagctc ccggtcggga gtgacgtaat ttgaggaata atgatgaaaa
    22201 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
    22261 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
    22321 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
    22381 aacgtccaaa cttattcaac tggcatatga ctcaacctga tgaaagcatt ctggtttctg
    22441 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acctggctga
    22501 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
    22561 agcagtacaa tatcaaacag aatggcgatg actttgttct gacgccgaaa gccagcaatg
    22621 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
    22681 cggtggagca ggacgatcag cgcagcagtt atcagctgaa atcccagcaa aatggggctg
    22741 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
    22801 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaaccc
    22861 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca acatttgctg
    22921 gctgtgggga agccgttgcc gcgcgctatc gaagccgggc atctgcattc tatgatcctc
    22981 tgggggccgc cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac
    23041 gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg cgaggcgatc
    23101 gaacgcgctc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt tgacgaagtt
    23161 caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
    23221 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
    23281 cgtgcccgtg tctatctgtt gaaatccctg agtacagagg atattgagca agtactaact
    23341 caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa
    23401 acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
    23461 gaaatgatgg cggatatggc cgaagtcgat gatagcggta agcgggtcct gaagcctgaa
    23521 ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt
    23581 tacgatctga tttccgcatt gcataagtcg gtacgtggta gcgcacccga tgcggcgctc
    23641 tactggtatg cgcgaattat taccgccggt ggcgatccgt tatatgttgc gcgtcgttgc
    23701 ctggcaattg cttccgaaga tgtcggcaat gccgacccac gggcgatgca ggtggcaatt
    23761 gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
    23821 gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc
    23881 gcgctggccg atgctcgcga acgtccggat tatgacgtgc cggttcattt gcgtaatgcg
    23941 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
    24001 gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat
    24061 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
    24121 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
    24181 gttaatgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
    24241 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
    24301 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga ggagcgtcgt
    24361 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
    24421 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
    24481 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
    24541 gatatcgcgc taaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
    24601 aatgacaacg ttgaagtcag tcgctggggc accccgcgtg agtttgactt tgaagttcgt
    24661 gaccatgtga cgctgggtga aatgcactct ggcctcgact ttgcagccgc agttaagttg
    24721 actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
    24781 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
    24841 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
    24901 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaacg
    24961 gcagaagttc cgctgaccaa cctggtacgc ggtgaaatca tcgatgaaga tgatctgcca
    25021 attaagatga ccgcccacac cccatgtttc cgttctgaag ctggttcata tggtcgtgac
    25081 actcgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
    25141 ccggaagact caatggcggc gctggaagag atgaccggtc atgcggaaaa agtcctgcag
    25201 ctgctgggcc tgccgtaccg taaaatcatc ctttgtaccg gcgacatggg ctttggcgct
    25261 tgcaaaactt atgacctgga agtatggatc ccggcacaga acacctaccg cgagatctct
    25321 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg ccgcagcaag
    25381 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtcttgc tgttggtcgt
    25441 acgcttgttg cagtaatgga aaactatcag caggctgatg gtcgtattga agtaccagaa
    25501 gttctgcgtc cgtatatgaa tggactggaa tatattggct aatacccaat ttttctgaat
    25561 ctaaaaagcg cctgcgggcg ctttaagtag aggtttaatc atttgaaaat aaatggattt
    25621 tatttttaag tgtccatata acgtccatct ttgaatgcaa aaagcccaca gcttctatgc
    25681 tgtgggcttt gttttttggt gtcctcatga gtgagggaac caaaaagaaa acccgcagtt
    25741 tttacgctgc gggcttgttg ttcacgtcaa ctggaaatag ggtgcctttt cgacctttac
    25801 cctggcaacc gattgacggg gaagttgcct tccccgtcgc ggtttcctta ctgcttacac
    25861 tgtaagaacg ccgcaaactc cgctccccag aaactcatcc gtatttcaca cagcgaaccg
    25921 tgcagcatcc agatgatgag gattgccgtc acgcagaatg tgatggccgt aagcgatttt
    25981 tgcgacatag cgcttgctcc tttttcgggg aggcgctaac ctatcacttg ctgagggtag
    26041 atggttaggg cctcgggtta acgttaaagt tgactcgggg cctttccaca tctggccttc
    26101 gggtattccc tccgaccatc agccgaaagg cacccgcacg taatctatcg cttttttgtt
    26161 actccggcaa ttctgcctgt taattctgag ataaaggcaa actcatctga ttgtttcccc
    26221 tgtgtgaagc tggcagatca tgccacggga taccttctga agagtgaacg ccggaggcgt
    26281 gttttgatgt gaatttatgg aaagcttcca gtgttgagaa gcatacgccg cattccagat
    26341 tgttacactg gtaatacttt tgccgcacgg tgtttgaatc attttccgga cgactggtgc
    26401 ggatacgggc agatgcgcca caaagcggac aacggaacat agcgacctcc cttaacgtgg
    26461 tgctgcctat tctaagttgc tcactctgtt tccgctatcc attccgggat ttttgcctca
    26521 agctcaagct gcgtggtaaa accgctgtta tcaatggtgt gctcggcttt tgcaatgatc
    26581 cagtcctgat tatcaatctc gcttttaaat cctgttaccg tgccatgcat ttcggggtag
    26641 agttctgcac gtccacgcgc cagcgtgatg gagaatgatg cggctccgcg ttgtagttgc
    26701 tgccactttg ccgccgctgc gcgtcttgct gcctgctcgt tctgataagt cttgcgtaac
    26761 acaaacacat tgccttccgc gccttccata taatcacctt cgcggctgct gcttttctcc
    26821 tttttgggtt ttggcggttt acggcgtttc acgctgactt ttttcttttt cccgtaatta
    26881 agatcaagcc agtaagcgcg tacccccgta tacgcttcgc ggtcagcaat gcggaactga
    26941 tggcgatcgc cgctgctgcg tgtgatggca aacgatggca acggctggcc ctgtgcgttc
    27001 acgccaccgc cgggcatgat gaataacaga ttaccgcttt ttaccgtggt gattgcgccc
    27061 agcatttccg ccatgcgcgt aaggaaggac atgtcgcttt cttcggtctg gtcggcgtgg
    27121 tcgatttcga tatccatcag catttcgctg atttgcggtt tcagaccata ccgatgagcg
    27181 atggcggata ccacacgctc aacggtcaca tcatgccagg acacctcacg tttaacgtta
    27241 aattcatccc gaaaatctgc gcttctggct gaaacagtca gcctgtccgg cggtccttcg
    27301 tgagcgattt catcaacaat gtaagtgcct ttttctgtca gcggttctcc tttccagcca
    27361 atgagaaccg tcaggcgcgc gccccgtggc ggtagctgca actgaccatc cgcatcatcc
    27421 agcgtgatgg tgagctggtc cgcctcaaat ccccggttgt cggtcagtga caggctcatc
    27481 aggcgctctg ccacgcctga cagcgtttta ccctccgcga gaatatcaaa atccggcatt
    27541 ttcacggggt ctgtgccctg actgagcaat tgcatggtgg tgtcggtcat ctgctccctc
    27601 cctgtgcggc atggtcgcat gtgcgtgcgg agggggttac tgctttttgt tgtcgccgtg
    27661 gcgggagaac ggcgcagggg tgagattacg cgcgtggtgg gtgatgattg ttgccgaatc
    27721 atttaacgga tacaaggggc tgaagctatg agtgaaactc gttttcatgg tgcccgtgtc
    27781 acggaaagta ccgacctggt aacagcgatt aatgatgttg attccagcgt tatcggtatc
    27841 gtggcaacgg cggatgatgc ggatgcggag ctgttcccgc tgaacaagcc cacactgctg
    27901 acccgcgtca atgacgtgct gggaaaatgc gggactacgg ggacgcttta tcgtgcgctt
    27961 aaggctatcg cagaccaggt gagcacaaaa gtgatcgtcg ttcgcgtggc tgaacacaaa
    28021 gaagaaggcg gtaggacgca ggatcaactg gttatcggtg gttctgaatc tgacggcagc
    28081 tatacgggga tgtatgcgct gcttgttgca gagcaggatg aaagcatcgg ataccgtccg
    28141 cgtattctgg ccgcgccgga gctggacacg gaggcggtga caaaatccct gtgcgtgatt
    28201 gcgggtaaac tgcgcgcgtt tgtgtatgcc acatgtcatg gttgtaacac gatggctgag
    28261 gcgattacct accgccagaa attcaacgaa cgtgaggtga tgctcttatg gcctgacttc
    28321 atcgcctaca acccgaaaag tggcaaaaac gaaacgttcc ccgcgcctgc ttatgcgtgc
    28381 ggccttcgtg cgtacattga ccatgagcag ggctggcaca aatcgctgtc caacgttccg
    28441 gttaaaaatg tgctggggat gtcgaggcat gtgttctggt cgttacaggc cgaagacagt
    28501 gatgccaaca gcctcaacaa caaagaaatc acgaccatta ttcgtcgtaa cgggttccgc
    28561 ttctggggca accgcacacc ggaaacgaac gcctacatct ttgaggtgta tacccgaacc
    28621 gcacaggtgc tggctgattc aattgcggaa gcgcagtttg aaaccatcga cagtccactg
    28681 acgcctgcga acgtgaagga tgttatcagt gccatcaggg caaaattgga ttcgctggtt
    28741 actgccggga aactgattgg cgcggagtgc tggtatgacg tggtggataa cggcaccacg
    28801 gatttacgtc aggggcgtgt gcgtattcgc tacaaatata cgcccgttcc cccgctggaa
    28861 gacatggagc tttaccagac gtttactgat gaattctttg gtcccgcatt tgcggtgctg
    28921 ggaggtgcct gatggctgta ccaaaacatc ttcgcttttt tacgttgttt gtggatggtg
    28981 aaaacgaagt gggtaaagtg acatccgtca cgccgcccaa actgacgcgc aaaaccgaca
    29041 gctatcgtgg tggtggcatg atgggagcag taagtattga tctcggtctg gacgactccg
    29101 cgcttgatgc gagctttgtc atggggggag ctgttcgtgc actgttcctt aagtatggcg
    29161 gcacgattga cggcacgctg ctgcgttttg cgggtgaata ttacaccgat gcagaaagcg
    29221 atctgtatga aatcgagatg cgcgggcgtg tgacggaaat tgatatgggg gaggccaaac
    29281 agggcgaagc cacatcacac acttacgctg tcaaaaacac ctactacaag ctgagcgtta
    29341 acgatcgccc gctgtgggaa atcgacctgt tgaaccacat ctaccggaag gacggcaagg
    29401 acattgtgcc tgaccgcatt cgttccgcgc ttgggcttgg ctgataagta atatgcaggc
    29461 ggcgcagtgc gtcgcctctg actgaaaagg agacaactga tgaaagatat cgatactgaa
    29521 acccggaata acaccgtagc ggatgatgtg acgtcaggtg aggatatggc tgtcgaacgt
    29581 ggtgtaaaac tttcccggcc aattgagcgt ggtgccgaaa aaatcacata tgtggagatc
    29641 accggggcta ttgagcaggc gggatccctg cgtggtctgt cgctgtctga tgtgctgaat
    29701 ctgaaagcgg ataccatgtt cacgctgttg cctcgcgtga cctcgccacg actggatgaa
    29761 gtgatgatta aaaaaatgtc gtcacgcgat tttattcagt tgtgcgctgt ggctgtaaat
    29821 tttatgagcg agccagactc tggcgcgaag agcgtgcagg agacggcagc gtaatcaccc
    29881 tggtgtgctt tgagcacatc gaagatctgg tggcggatat tgccgccatt ttcaactggt
    29941 cgcccgccga aatcttcatg atgacgcccg gcgaagtggt tagctggcgt gagcgggcgg
    30001 cacttcgcag cgggaatgca gataatgaag actcttgaca tccgggtcgc tttcagcgcc
    30061 gttgacaggc tgacccggcc tgccgaaaac gcccgccgcc tgatggggca gtttggtgac
    30121 tccattcagc gaacgcaggg ggcgatcaaa aatctcgagc gtcaggcgcg ttcatttgag
    30181 cgcgcccgcg acgctgtcag taaagcggat gcgggtatcg tgaaagcacg acgccagctt
    30241 aacgccctta atcagttaca acgcacgggt acagtgctca gcgaaaaaca acaaaagctg
    30301 atgcagcagt taagcacccg gcttgaacgc ctgaatgaat cgcgcacacg ggaaattcag
    30361 aaaatgcggg agcttggcgg agaactgaaa cgccacggca tttccctgac aggcagcgat
    30421 aacaccatcc agcaggccat cagacgcacc gaacaataca acaaccagct tgaacgcgaa
    30481 cggcaggcgc ttgcgcgtgt aacgcaggct cgtgagcggt attcgcgcgc gcaggaaacc
    30541 gcgggaaaac tgaaaacagg tggtgcgctg gcaattggtg cggcagcggc gggcggctat
    30601 gctgccgggc gttttttgca gcccgcgatc gggttcggca aagagatgtc ccgcgttcag
    30661 gcgctgacgc gaatcgacaa aaacagcccg cagtttaagg cgctgcgtga gcaggcgtta
    30721 aaacttggct ctgaaacaca gtttactgcg agtgatgccg ccagtgggca gagctttctg
    30781 gcaatggctg gttttactcc gcaggccatt caggccgcat tgcccggtgt tcttaatatg
    30841 gcgctggcag gtggcgtcga actcggcgag acggctgata taggctccaa tatcctcaca
    30901 cagttcaacc tgacagccga tcaaatggac cgggttggcg atacgctgac agcggcattc
    30961 acccggacca atactgattt acgcgcgctg ggcgaaacca tgaagtatac cggtccggtt
    31021 gccgcaaaac ttggtatcag tcttgaagaa gcggcagcca tggccgggat gcttgccaat
    31081 aatggtcttc gtggaagcga tgctggtacg gccatgcgcg caagtctgtc ccgccttgca
    31141 tcaccgccaa aagctgcggc tgatgcgctg aaagagctgg gggtgtcagt tgctgacgcc
    31201 agaggcaaaa tgcgcccgat ggaggatgtg ctgcttgatc tctataaggc gacacaaaaa
    31261 tacggacagg tggaccaggt ttccttcttc aaggacatcg ccggagaaga ggcgttcgtt
    31321 ggtttgcaga cgcttgttgc ggcggctggt tcaggagagc tgcaaaaact gaccagagaa
    31381 ttgcaggggg caaggggaga ggccgatcgc gttgcaaaag taatggccga taatcttgat
    31441 ggggacctga aaaatctcga cagcgcatgg gaaggtcttc gtattcgcat cagtgatctg
    31501 gttgacggtc cgctgcgttc tgtcacgcag tggctcacgc gggtgcttga aaaaatcacc
    31561 tcgctggcgc aggcccatcc ggtactgacg cgccagctac tgatagcagg cggtgcgttg
    31621 ctggcaatga ctgcaacggt tggctcgttg tcgctggcta ttggtgtgct tgctggcccg
    31681 ctggcaaaac tgcgtcttgg cttttccctc ctgaccggtt caatgaatgc tgtcagactc
    31741 ctgccagcac tatggggaat ggtgacgggt tccgtttcgt tactgggggg cgctatcggg
    31801 gcgttgttca gtccggtcgg ccttatcgtg gctgcgcttg ccggagctgc cgttctcatc
    31861 tggaaatact gggatcccat cagggcattt tttgccgggg tgttcagcgg gattatggaa
    31921 aggctgaccc cgttgcgcga aacctttgaa cggtttggtc ctgtttttga cgcaatcggg
    31981 agtgggatca gccaggtgtt taactggttt aaatcgctgc tgtctccgat ggagtccagc
    32041 aaggaaactc tggataaatg taccagtgct ggcgagatat tcggtaacgt tcttggcggt
    32101 gcgttacaac ttgttctgac acctgcaaaa atgttgctgg atacgctggc gtggatactt
    32161 gaaaagcttg gtgtgcttcc ggatgaagcg gaaagggcgc gcaagaaaat cgaagacgca
    32221 cagcgtgcgg ccattcttca ggacaaggtt gccttgcttc agggggacct ggcgaaaatc
    32281 aatccgccga agcctgtgga aaatggcaat ggcaccggag gtgataaccc caaagacaat
    32341 aaaccgctca cagacagcaa taccggtacg ctgcgcagac tcagcaaaat tgctgataac
    32401 acaggtaagc tggttgagga gacgaaaaaa cgcattggcc ccggcgatat tgtctttaaa
    32461 aacctgcccc gagcacttgc tgttcgtggg gagtggcagg agcggaagat tgcgcaggtc
    32521 agtaagcctg cccccgcaat taatatcaca cccgtggtcc cggctccgct gcctccggcg
    32581 ctggtccctg ttgttgcggc cagctcccgc ccggtggcgg aggctatacg atctccagtg
    32641 gcatcagttc ctgtaacttc ccgtaaccgg gagcctgttg cctccggatt tggtggtgaa
    32701 attcatgttc atctgcataa cgttgttacg cagaatcccc gcgaactggc gaaactggtc
    32761 ggtgaaatgg tcagggcaga aatggaacgg cgcgcccgtg ccgggcgtgg cagtttttac
    32821 gataaagatt gaggagtcat ggccatgatg atgatctacg gcatgtttgt ttttgagctg
    32881 cgcacgctgc cgcatcagca gttacagcaa aacaaaagct ggcggcatgt gaaaaatgaa
    32941 cgcgttaacc gttcagcaag ctggcagtat atcggtgcag gtgatgatcg catcgttctt
    33001 tctggtgtgc tttatcctga aattacaggt ggcgaagtgt cgctgtcgct gctgaccacg
    33061 caggcgtata caggacgacc ctggcctttg attgatggcg tcgggcagat ttacggcatg
    33121 tatgtcctga ctgaaacgaa tacgacccgc tccgagtttg atcgctacgg taaggcgaaa
    33181 aagatagaat tttcactgac tcttgaacgc tgtgatgagg atttgcggga gcgcctgcaa
    33241 tcctcatcgt tcagtgatat gctgtccggc ttcaaagata aggtgacatc atctcttaac
    33301 agcgcggcca gttcagttaa agggctgttc tgatttaaca caaaaaccgc taatggtcag
    33361 attagcggtt ttcattttcc tgagtctgcc cggttgtttc ttcagtctgt atatcgccta
    33421 cagggtgata acgataaatc gtcgatgtgc caatgccgta aattattgcc agttgttttc
    33481 tgtcgtgccc gtttttgatt agccttgcta tttgctcatg ctgttctttt gtcagcttcg
    33541 gtcgacgtcc gcctgtgcgc ccccttgcgc gcgctgccgc cagcccggcc agtgtacgtt
    33601 caacaattaa ttcacgttcc atttcagcca gggcacccat cacgtggaag aaaaaacgcc
    33661 ccattggaga agatgtgtct atgctgtcgg tcagactgcg aaaattaatc cctcgctccc
    33721 gtagttcccc gacgagagaa atcagatgtt tcatgcttcg cccgaggcga tccagtttcc
    33781 agacaaccag cgtgtcacct ttttgaaggc gctttaaagc gcgttttaat cccggtcggt
    33841 ctgtctttgt cccgcttaat ttatcttcaa atatttgttc acatcctgca caaacaagag
    33901 cgtttcgttg caggtctgta ttctggtcat ttgttgatac ccttacatag ccaatcagca
    33961 cactgaatct cccgtccaaa agcgcaaatc atgccatgca ggccagaaac ggccattatt
    34021 taa
//
LOCUS       DAPQZD010000038        34017 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPQZD010000038 DAPQZD010000000
VERSION     DAPQZD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.038"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            14..760
                     /gene="mipA"
                     /locus_tag="RO454_001748"
     CDS             14..760
                     /gene="mipA"
                     /locus_tag="RO454_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDY0933593.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            850..1704
                     /gene="yeaE"
                     /locus_tag="RO454_001749"
     CDS             850..1704
                     /gene="yeaE"
                     /locus_tag="RO454_001749"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDY0933594.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1755..2639)
                     /locus_tag="RO454_001750"
     CDS             complement(1755..2639)
                     /locus_tag="RO454_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDY0933595.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(2723..3718)
                     /gene="gapA"
                     /locus_tag="RO454_001751"
     CDS             complement(2723..3718)
                     /gene="gapA"
                     /locus_tag="RO454_001751"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDY0933596.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            4060..4473
                     /gene="msrB"
                     /locus_tag="RO454_001752"
     CDS             4060..4473
                     /gene="msrB"
                     /locus_tag="RO454_001752"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDY0933597.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            4515..4787
                     /locus_tag="RO454_001753"
     CDS             4515..4787
                     /locus_tag="RO454_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDY0933598.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            5157..6233
                     /locus_tag="RO454_001754"
     CDS             5157..6233
                     /locus_tag="RO454_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDY0933599.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            6260..7639
                     /locus_tag="RO454_001755"
     CDS             6260..7639
                     /locus_tag="RO454_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933600.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            7656..8699
                     /locus_tag="RO454_001756"
     CDS             7656..8699
                     /locus_tag="RO454_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDY0933601.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            8720..9556
                     /locus_tag="RO454_001757"
     CDS             8720..9556
                     /locus_tag="RO454_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDY0933602.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            9561..10508
                     /locus_tag="RO454_001758"
     CDS             9561..10508
                     /locus_tag="RO454_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDY0933603.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            10518..11498
                     /gene="ydjG"
                     /locus_tag="RO454_001759"
     CDS             10518..11498
                     /gene="ydjG"
                     /locus_tag="RO454_001759"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDY0933604.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            11635..12393
                     /locus_tag="RO454_001760"
     CDS             11635..12393
                     /locus_tag="RO454_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0933605.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            12511..13869
                     /locus_tag="RO454_001761"
     CDS             12511..13869
                     /locus_tag="RO454_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933606.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(13962..14603)
                     /gene="pncA"
                     /locus_tag="RO454_001762"
     CDS             complement(13962..14603)
                     /gene="pncA"
                     /locus_tag="RO454_001762"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDY0933607.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(14614..15630)
                     /gene="ansA"
                     /locus_tag="RO454_001763"
     CDS             complement(14614..15630)
                     /gene="ansA"
                     /locus_tag="RO454_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDY0933608.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQKLDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(15762..16472)
                     /locus_tag="RO454_001764"
     CDS             complement(15762..16472)
                     /locus_tag="RO454_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDY0933609.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(16588..18444)
                     /gene="sppA"
                     /locus_tag="RO454_001765"
     CDS             complement(16588..18444)
                     /gene="sppA"
                     /locus_tag="RO454_001765"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDY0933610.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            18605..19156
                     /locus_tag="RO454_001766"
     CDS             18605..19156
                     /locus_tag="RO454_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDY0933611.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19273..20316
                     /gene="selD"
                     /locus_tag="RO454_001767"
     CDS             19273..20316
                     /gene="selD"
                     /locus_tag="RO454_001767"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDY0933612.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGVSFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20321..22276
                     /gene="topB"
                     /locus_tag="RO454_001768"
     CDS             20321..22276
                     /gene="topB"
                     /locus_tag="RO454_001768"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDY0933613.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(22353..23696)
                     /gene="gdhA"
                     /locus_tag="RO454_001769"
     CDS             complement(22353..23696)
                     /gene="gdhA"
                     /locus_tag="RO454_001769"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDY0933614.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            23932..24204
                     /locus_tag="RO454_001770"
     CDS             23932..24204
                     /locus_tag="RO454_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDY0933615.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(24170..24577)
                     /gene="nudG"
                     /locus_tag="RO454_001771"
     CDS             complement(24170..24577)
                     /gene="nudG"
                     /locus_tag="RO454_001771"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDY0933616.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            24664..25284
                     /locus_tag="RO454_001772"
     CDS             24664..25284
                     /locus_tag="RO454_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDY0933617.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(25293..26600)
                     /gene="ynjE"
                     /locus_tag="RO454_001773"
     CDS             complement(25293..26600)
                     /gene="ynjE"
                     /locus_tag="RO454_001773"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDY0933618.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(26667..27320)
                     /locus_tag="RO454_001774"
     CDS             complement(26667..27320)
                     /locus_tag="RO454_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDY0933619.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(27320..28855)
                     /locus_tag="RO454_001775"
     CDS             complement(27320..28855)
                     /locus_tag="RO454_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0933620.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVVKWVGYVRQ
                     GLR"
     gene            complement(28828..29994)
                     /locus_tag="RO454_001776"
     CDS             complement(28828..29994)
                     /locus_tag="RO454_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0933621.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(30004..30552)
                     /locus_tag="RO454_001777"
     CDS             complement(30004..30552)
                     /locus_tag="RO454_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDY0933622.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(30552..31259)
                     /locus_tag="RO454_001778"
     CDS             complement(30552..31259)
                     /locus_tag="RO454_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDY0933623.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(31273..31950)
                     /gene="ydjY"
                     /locus_tag="RO454_001779"
     CDS             complement(31273..31950)
                     /gene="ydjY"
                     /locus_tag="RO454_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDY0933624.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(31955..32665)
                     /locus_tag="RO454_001780"
     CDS             complement(31955..32665)
                     /locus_tag="RO454_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDY0933625.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(32832..33638)
                     /gene="xthA"
                     /locus_tag="RO454_001781"
     CDS             complement(32832..33638)
                     /gene="xthA"
                     /locus_tag="RO454_001781"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDY0933626.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         8485 a         8262 c         8810 g         8460 t
ORIGIN      
        1 ggaatgatta attgtgacca aactcaaact tctggcactt ggagtgctta tcgcaacgtc
       61 tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga
      121 acacccatat aaagattacg ataccgatgt ttacccagta ccggtaatca actatgaagg
      181 cgataacttc tggttccgtg gcttaggtgg tggttactac ctgtggaatg acgcgacgga
      241 caaactttca attaccgctt actggtcgcc gctttacttc aaagctaaag acagtggcga
      301 tcaccaaatg cgtcacctcg atgaccgtaa gagcaccatg atggctggtc tgtcttatgc
      361 tcactttacc cagtacggtt acctgcgtac caccctggct ggcgataccc tggataacag
      421 caacggcatc gtctgggata tggcctggtt gtatcgttac accaacggtg gcctgaccgt
      481 gactccgggt attggtgtgc agtggaacag cgaaaaccag aacgaatact attatggcgt
      541 atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat aacccgaacg acagctggag
      601 cccttacctg gagctgagcg ccagctacaa cttcctcggc gactggagtg tttacggtac
      661 cgcgcgctac acccgtctgt ctgatgaagt tactgacagc ccaatggtgg ataaatcctg
      721 gactggcctg atttctaccg ggatcaccta caaattctga taatgcatct ttccagggca
      781 ttttgtgcat aaaaacaggg cgtaatgccc tgttttgcat tacggtggtg caatacctgg
      841 gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg atgtctcact gccagccata
      901 gggcagggaa catggtatat gggcgaagat gccagtcagc gtaaaacaga agttgctgca
      961 ctacgcgcgg gcattgaact cggtttaacc ctcattgata ccgccgaaat gtatgccgat
     1021 ggcggtgccg aaaaagtggt tggggaagca ttaatcggtc tgcgtgagaa cgtctttctc
     1081 gtctctaaag tctatccgtg gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc
     1141 agtttacgcc gtctcaatac tgattatctc gatctctact tattacactg gtctggcagt
     1201 ttcgctctcg aagagactgt cgcagcgatg gaaaaattga tcgcccaggg aaaaatccgc
     1261 cgctggggcg tttctaacct tgattatgcc gatatgcagg aactctggca gctgccgggg
     1321 ggaaatcagt gtgccactaa tcaggtgctt taccatctcg gttcacgagg gattgagtac
     1381 gatctacttc cctggtgcca gaaacagcag atgccggtga tggcttacag tccattagcc
     1441 caggccgggc ggttgcgcaa tggactgtta aaaaatgcgg tagtcaacga aattgcacat
     1501 gctcacaata tcagtgcggc acaagtattg ttggcgtggg taatcagcca tcagggtgtg
     1561 atggcgattc caaaagcggc aactgtcgca catgtccaac aaaatgcggc tgtgcttgag
     1621 gttgaacttt cgtcagcgga attagttatg ctggataaag cgtatccggc accaaaagga
     1681 aaaactgcgc tggatatggt gtgatgttga ttcgtgccgg atgcatcgca cccggcacac
     1741 aatattaacg caaattaacg tttagcaacg cgaatggatt gcgccagatg tgcaggtttc
     1801 tctttggtga ctttttgcgt ttctgaagcg taagccgttt ctacacaaac aaaggtcttg
     1861 tagccatcat ccggcatatc gcccatgcta actgaaagcg ccggtcctgg gttccagcct
     1921 acaacgttca gatgatgctg gtggcctacg gcgataatac gattcagcgc ttcgtcatta
     1981 attacgctgc aatcttgcgg attcagatac acgcgatcgg tacggtcagg gaaggtctga
     2041 ataccgtcgg tcagtacatc ttctttcgcg tcatttactt tatcaatgaa gcgatcgcct
     2101 agtccactga cgcttacctt agcgatatca cccacgttaa agtaggtatg cagagcagag
     2161 gtagtttcaa attcgccgtg tgcttcaaga tcgatttcac aggttttacc cacgtggaaa
     2221 tgcgctaaca gcgtaaagtc gtgcggccag aattttttcg tctcttcgct ttgtgtcaat
     2281 tcaaaagtca gcgctacacc atcagcatct tcacgatgtg atttcagcgt ccacggcagg
     2341 ttgcgggcaa aaccgtgcgc aggcagacct tgttgtgcag ccggaccaaa ccacggccag
     2401 caaaccggta tgccaccgcg aatggcgacg ccatttttga acggtgtgtt gttactcaac
     2461 cacagaactt cttcttcacc cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca
     2521 aaagaggctt ttacctgggg atgatcgacc acaatgaggt ccagttcatc cagtttacga
     2581 cgggagagga caggggagat ttgttcgatg accggaaggg caaaaatttt cttaatcatg
     2641 acgcagtcct ttaacttcat tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt
     2701 tagatcacag tgtcatctca acttatttgg agatgtgagc gatcaggtcc agaactttgt
     2761 tggagtaacc ggtttcgttg tcgtaccagg ataccagttt cacgaagttg tcgttcagag
     2821 cgatacccgc tttagcatcg aacacggaag tgcaaacttc gccgttgaaa tcggtagata
     2881 ctacgtcatc ttcggtgtag cccagaacgc ctttcatttc gccttcagca gcagctttaa
     2941 cggcagcttt gatctgctcg taagttgcag ctttttccag acgaacggtc aggtcaacta
     3001 cggatacgtt cggggtcgga acgcggaacg ccataccagt cagtttgcca ttcagttctg
     3061 gcagtacttt acctacagct ttagcagcac cggtagaaga cgggatgatg ttctgggaag
     3121 cgccgcggcc gccgcgccag tctttgtgag acgggccatc aacggttttc tgagtagcgg
     3181 tagtagcgtg aacggtggtc atcagacctt cgatgatgcc gaagttatcg ttgataactt
     3241 tagccagcgg agccaggcag ttggtggtgc aggatgcgtt ggaaacgatg tcctggccag
     3301 catatttgtc gaagttagcg cctttaacga acatcggagt gttgtcttta gacggaccag
     3361 tcataaccac tttcttcgca ccagcggtga tgtgtttacg agcagtttcg tcagtcagga
     3421 acagaccagt tgcttcagcg acaacgtcaa caccaacttc gtcccatttc aggttagccg
     3481 gatcacgttc agcggtaaca cggatttttt taccgttaac gatcagatga ccgtctttca
     3541 cttcaacggt accgtcgaaa cggccgtgag tggagtcata tttcagcatg tatgccatgt
     3601 aatcagcgtc taacaggtcg ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag
     3661 cacggaaaac aatgcgaccg atacggccaa aaccgttgat acctactttg atagtcatat
     3721 attccaccag ctatttgtta gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg
     3781 cagcgtcaag cggaatcgtg tcaatcattg cgacaaatca atcctgtgcc taagcattac
     3841 gcgactgact cgcctcactc ttcctttggg cttgagacca catggggtcg gcgccccgaa
     3901 ttttaaaggg caattacgat aaaaatgtga ttttcatcac gatttaggtg cagccatttc
     3961 gtcgagatca agtttaggac aaaattttgc cagttggtgt taatgttttg ttagaatcag
     4021 tcacgtaatg tgagcacgat taaagtgaga tgtgagcaaa tggctaataa accttcggca
     4081 gaagaactga aaaaaaattt gtccgagatg cagttctacg tgacgcagaa tcatgggaca
     4141 gaaccgccat ttacgggtcg tttactgcat aacaagcgtg acggcgtata tcactgtttg
     4201 atctgcgatg ccccgctgtt tcattcccaa accaagtatg attccggctg tggctggccc
     4261 agtttctacg aaccggtgag tgaagaatct attcgttata tcaaagactt gtcacatgga
     4321 atgcagcgca tagaaattcg ttgcggtaac tgcgatgccc atctgggcca tgtctttcct
     4381 gacgggccgc agccaacggg cgaacgttat tgtgttaact ctgcttcttt acgcttcact
     4441 gatggcgaaa acggcgaaga aatcaacggt tgaaaaaacg attcagcaac ttattccaca
     4501 ggagcggaaa aattatgaat cttgatgaca ttatcaacag catgacgcct gaggtatacc
     4561 agcgtttgtc gaccgccgtt gaactgggga aatggcctga tggcgttacg ttgaccgagg
     4621 aacaaaaaga gaactgcctg caactggtga tgctgtggca agcccgccat aataccgaag
     4681 cacagcatat gacaattgac acaaatggtc agatggtcat gaagagcaaa cagcagttaa
     4741 aagaagattt tggtatctca gcaaagccta ttgccatgtt taagtaagtg gtttatcccc
     4801 tttttgccgc ggttataaag gggatagcca acaatgaccc cacgactcat ctcaattatc
     4861 tcgccccgac ctggctaagt cccagtccga tctcttgcag tcgcgttcac acctgaatga
     4921 cttctcctga acttaagtct caggcctatt tggccgggaa tccctctcga atttgctctt
     4981 tttgcgtccc gccttccatc aacctgtaac aaccccagcc attttctgcg taattgtgat
     5041 ctaagttgta aaacgcagat cattatctgt gttttacaga tttaatgttg acctcaaaaa
     5101 tcatgtgggt aagtatgatg accatctaag caaaacaaaa cacagaggat gacgcaatga
     5161 aagcactggc tcggtttggc aaggcttttg gtggctacaa gatgattgat gtcccacaac
     5221 ccatttgtgg cccggaagat gtcgtgattg aaattaaagc cgcggcaatc tgcggcgcag
     5281 acatgaagca ctacaatgtc gatagcggtt cggatgagtt taactctatc cgcggccatg
     5341 agttcgcagg ttgtattgcg caggttggtg aaaaagtcaa agactggaaa gtggggcaac
     5401 gcgtcgtgtc ggataacagc ggtcacgttt gcggcgtttg cccggcctgt gaacaaggtg
     5461 attttctgtg ttgtacagaa aaagtaaacc ttggtctgga taacaatacc tggggcggtg
     5521 gtttttccaa atattgtctt gttcctggtg aaattctcaa aattcatcgt catgcgttgt
     5581 gggaaatccc tgatggtgtt gattatgagg acgcagccgt acttgaccct atctgtaatg
     5641 cctataaatc catcgcgcag caatcgaaat tccttccagg tcaggatgtg gtcgtcatcg
     5701 gcactggccc actcgggctg ttctccgtac aaatggcgcg gattatgggg gcggtaaata
     5761 tcgtcgtcgt tggtctgcaa gaagatgtaa cggtccgctt cccggttgca aaagaactgg
     5821 gtgcgacggc agtagtaaat ggttctaccg aagatgtggt ggcacgctgc cagcaaattt
     5881 gtggcaaaga caatctgggg ctggtgattg aatgctccgg tgccaatatc gcattgaaac
     5941 aagccatcga aatgctccgc ccgaatgggg aagtagtacg cgttggaatg ggcttcaaac
     6001 ctcttgattt ctcgattaat gacattaccg cctggaacaa aagcatcatt gggcatatgg
     6061 cctatgactc cacttcatgg cgtaacgcta tcaggctatt agccagcggc gctatcaaag
     6121 tcaaaccgat gatcacgcat cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga
     6181 tggtcgataa aactgcaatc aaagtgatca tgacttacga ctttgatgaa taactactct
     6241 cctgaaggaa cgttatcgca tggaacagat aacaaaaccg cattgtggtg cacggctgga
     6301 tcgcttaccg gattgccgct ggcattcgtc gatgtttgcc atcgtcgcgt ttggcctgct
     6361 tgtctgctgg agtaatgccg ttggtggctt gatcctcgcg cagctgaaag cgttgggctg
     6421 gacagataat tccaccactg ccacattctc agcaatcacg accgccggaa tgtttctcgg
     6481 tgcgctggtg ggcggcatca tcggtgacaa aaccggtcgc agaaatgcgt ttatcctcta
     6541 tgaggccatt catattgcct cgatggttgt cggtgctttc tcaccgaata tggatttcct
     6601 tattgcctgc cgttttgtga tgggcgttgg gctgggagct ttactggtta cactgtttgc
     6661 tggtttcacc gaatatatgc ccggaagaaa tcgcggaacg tggtccagtc gggtttcctt
     6721 tattggtaac tggtcatatc cgctctgttc attgattgcg atgggactca cgccgctgat
     6781 tagtgcagag tggaactggc gggtacaact gcttatccct gcaatattgt cacttatcgc
     6841 tacggcgctg gcctggcgct actttcctga atccccgcgc tggctggaat cgcgcggacg
     6901 ttatcaggaa gccgagaaag tcatgcggag tatagaagaa ggcgtcatac gccagacggg
     6961 taaacctttg ccgcctgtgg ttattgctga tgacggtaaa gcaccacaag cggtgccgta
     7021 ttcagcctta ctgacaggag tattactgaa acgcgtgata ttaggctctt gtgtgctgat
     7081 tgccatgaac gttgtgcagt acacactaat taactggttg ccaacgatat tcatgaccca
     7141 gggaattaat ttaaaagact cgattgtttt aaataccatg agtatgtttg gcgcgccatt
     7201 tggtattttt attgccatgc tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg
     7261 gctattaatc ctgattgcgg tgctcggata tatctattca ctgcaaacca gtatgttgct
     7321 cataacgctg attggtttct tcctgattac tttcgtctat atgtacgttt gctatgcctc
     7381 ggcagtgtat gtccctgaga tctggccgac agaggccaaa ctccgtggct ccggtctggc
     7441 gaatgcggta gggcgaatca gtggtattgc cgcaccttat gccgttgcag tgctgctcag
     7501 tagttatggc gtaacgggag tctttattct tctgggggct gtttcaatta ttgtcgcaat
     7561 tgctatcgcc accattggaa ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga
     7621 tgcagtagcc aataaataat actggtggat tcaaaatgaa aaattcaaaa gcaatgttgc
     7681 aggtgccggg cacaatgaaa attatttcag cagaaatacc tgtacctaaa gaagatgaag
     7741 ttttgattaa agtggaatat gtcggtattt gtggttcaga tgtacatggt tttgaatcag
     7801 gcccgtttat tcctcctaaa gacccaaatc aagaaattgg cctgggtcat gaatgcgccg
     7861 ggacggttgt ggctgtggga agccgcgtgc gcaaatttaa accgggggat cgggtaaata
     7921 tcgaacctgg cgttccttgc ggtcactgtc gttactgtct ggaaggcaaa tataacatct
     7981 gcccggacgt tgattttatg gcgacacaac ccaactaccg cggcgcatta acgcactatc
     8041 tgtgtcatcc ggagagcttt acttacaaac tgccagacaa tatggacacg atggaagggg
     8101 cgctggtgga gcctgccgca gtcgggatgc atgccgcgat gctggcagat gttaaaccgg
     8161 gtaagaagat aattattctg ggagcgggtt gtattggttt gatgacgttg caagcgtgca
     8221 aatgcctggg agcaacggaa attgccgtcg ttgatgtgct ggaaaaacgt ctgacaatgg
     8281 cggaacagct cggcgcgaca gtggttatta acggcgcaaa agaagacact attgcacgct
     8341 gtcagcaatt taccgaagac atgggcgcag atattgtttt tgaaacagcg ggttctgcgg
     8401 tcaccgttaa acaggcacct tatctggtca tgcgcggcgg taaaattatg attgttggta
     8461 ctgtacccgg cgattcggca atcaatttcc tcaaaatcaa tcgcgaagtc actatccaga
     8521 cggtattccg ctatgccaat cgttatccgg tcacgattga agctatttct tcagggcgat
     8581 tcgatgtgaa atcgatggtg acgcatattt acgattatcg ggatgtacaa caggcatttg
     8641 aagagtcagt taacaacaaa cgcgacatta ttaaaggcgt tattaaaatt agcgattaat
     8701 taatacggga gaacagaaca tgctcgcaga tatcagatat tgggaaaacg atgccacaaa
     8761 taaacattat gcaattgccc attttaacgt atggaatgca gaaatgttga tgggcgttat
     8821 cgacgcagcc gaagaagcga aatcaccggt tattatttct tttggcacag gttttgtcgg
     8881 caacacctca tttgaagatt tctctcacat gatggtatca atggcacaaa aagcaacggt
     8941 gccggtaata actcattggg atcatggtcg gagtatggag attattcata acgcctggac
     9001 tcatggtatg aattcattaa tgcgtgatgc ttctgcattt gatttcgaag aaaatattcg
     9061 tttaaccaaa gaggctgttg atttcttcca tccgctgggt attccggtag aggcggaatt
     9121 agggcatgtc ggtaatgaaa ccgtttatga ggaagcgctg gcaggctatc actataccga
     9181 tcctgaccag gcggctgaat ttgttgaacg tacgggctgt gactcattgg ctgtcgccat
     9241 cggcaaccag catggggttt atacgtcaga gccacaattg aactttgagg tcgtcaaacg
     9301 cgtacgcgat gctgtttctg ttccgctggt tttgcatggc gcatcaggga tcagtgatgc
     9361 cgacattaaa actgcaattt cgttgggtat tgcgaaaatc aacattcata cggagctctg
     9421 tcaggccgca atggtagcag tgaaagaaaa ccaggatcag cctttcctgc atttagaacg
     9481 tgaagtgcgt aaagctataa aagaacgggc attggataaa attaaactgt tcggttctga
     9541 tggcaaagcg gaataataat atggataatc tcgacgttat ttgtataggt gccgctattg
     9601 ttgatattcc attgcaaccg gtcagtaaaa atatctttga tgtggattct tatcctcttg
     9661 aaagaatcgc aatgaccacc gggggtgatg caataaatga agcaacaatt atttctcgtc
     9721 tgggccatcg cacagcatta atgagtcgta ttggtaaaga tgccgcaggg caatttattc
     9781 tcgaccattg ccgcaaagag aatattgata ttcaaagcct gaagcaggac gtcagcatag
     9841 atacctctat taacgttgga ctggtgacgg aggatggcga gcggaccttt gtcacaaacc
     9901 gtaacggcag tctgtggaaa ttgaatattg acgatgttga ttttgcgcgg ttttctcagg
     9961 cgaaattatt atcactggcc agtattttca acagtcctct attggatggt cgagcgctaa
    10021 cagaaatttt tacgcaagct aaaacccggc agatgattat ctgtgccgat atgatcaaac
    10081 cgcggttgaa tgaaacgctg aatgatattt gcgaagcatt gagttatgtc gattatctgt
    10141 ttcctaattt tgccgaggca aaattactca ccgggaaaga gacactggat gaaattgctg
    10201 actgctttct tgcgtgcggc gtaaaaacgg tggtgattaa aacgggtaaa gacggctgct
    10261 ttatcaagcg tggtgacatg acgatgaagg tgccggcggt cgcaggaata accgctatcg
    10321 acaccattgg tgcgggcgat aactttgctt caggttttat tgcagcactg ttagaaggca
    10381 aaaatctgcg agaatgcgca cgctttgcca atgcaacggc ggctatctcg gttctaagca
    10441 tcggcgccac caccggtgta aaaaacagaa agctggtcga acaattgctg gaagaatacg
    10501 aaggataatg aaggcaaatg aaaaagatac ctttaggcac agcggatata acgctttcgc
    10561 gaatggggtt gggtacatgg gctattggcg gcggtcctgc atggaatggc gatctcgatc
    10621 gacaaatatg cattgatacg attcttgaag cccatcgttg cggcattaac ctgattgata
    10681 ctgcgccagg atataacttt ggcaatagtg aagttatcgt tggtcaggcg ttaaaaaaac
    10741 tgccccgtga acaggttgta gtagaaacca aatgcggcat tgtctgggaa cgaaaaggaa
    10801 gtttattcaa caaagttggc gatcggcagt tgtataaaaa cctttctccg gaatctatcc
    10861 gcgaagaggt agaagccagc ttgcaacgtc tgggtattga ttacatcgat atctacatga
    10921 cgcactggca gtcggtgccg ccatttttta cgccgatagc tgaaactgtt gcagtgctta
    10981 atgcgttaaa ggccgaaggg aaaattcgcg ctataggcgc tgctaacgtc gatgctgacc
    11041 atatccgcga gtatctgcaa catggtgaac tggatattat tcaggcgaaa tacagtattc
    11101 tcgaccgggc gatggaaaac gaactgctgc cgctatgtcg tgataatggc attgtggttc
    11161 aggtttattc cccgctagag cagggattgt tgaccggcac catcacgcgt gattacgttc
    11221 cgggcggcgc tcgggcaaat aaagtctggt tccagcgtga aaacatgctg aaagtgattg
    11281 atatgcttga acagtggcag ccactctgtg ctcgttatca gtgcacaatt cccactctgg
    11341 cactggcgtg gatattaaaa cagagtgatt taatctccat tcttagtggg gctactgcgc
    11401 cggaacaggt acgtgaaaat gtcgcggcac tgaatatcaa cttatcggat gcagacgcaa
    11461 cattgatgag ggaaatggca gaggccctgg agcgttaaat actcaacacg attcacctct
    11521 gtttctgtga aaactgtgat gaaaatctgt gattcaaaca ggttattttg aaagtaaaca
    11581 tcggttatgc gataattgcg ctaatgtgat gtaaaaagat acaggggtgt aatcgtggcg
    11641 gcaaaagaca ggattcaggc aattaagcaa atggttgcca acgataaaaa ggtgacagtc
    11701 tcaaatttga gtgggatttt tcaggtaacc gaagaaacca ttcgccgcga tcttgagaag
    11761 ttggaagatg aaggcttttt gaccagaacc tatggtggtg ctgttttaaa tacagcgatg
    11821 ttgacggaga atatccattt ttataagcgc gcttcatcgt tttatgaaga gaagcagctt
    11881 attgcacgca aggcactacc ctttgtcgac aataaaacca ccatggcagc cgattccagt
    11941 agtaccgtaa tggaattgct caaattgtta caggaccgta gtgacctgac gttgctaacc
    12001 aactcggcgg aagcgattca tgttctggct cagtcagaaa ttaaagtcgt ttcaacgggt
    12061 ggggaactaa acaaaaatac actttcactg caaggaagaa ttactaaaga gatcatcagc
    12121 cgctaccatg tcgatatcat ggtaatgagt tgtaaaggtc ttgatattaa cagcggcgcg
    12181 ctggactcta atgaagcgga agctgaaatc aaaaagacaa tgatccgtca ggcgacagaa
    12241 gttgcgttat tggttgatca ctctaagttt gatcgcaaag cttttgtcca gttagctgac
    12301 tttagtcata ttaattacat aataactgat aaatcaccgg gtgcagagtg gatagcattt
    12361 tgcaaagaca ataatatcca gctggtgtgg taatttaagc acacattgtc aatattattt
    12421 gttcctgcgc ctggaaataa aatgatgata aagatgcggc aagtgaattt gccgcaattg
    12481 ctaccttttt tagcaaatcc gaggcaccca atggaacaat atgatcaaat tggcgcaaga
    12541 ctggaccgct tgcctttggc ccggtttcat tatcgtatat ttggtataat aagctttagt
    12601 ctgttattaa cggggttttt gagttactcc ggtaatgtcg tcttagcaaa gctggtaagc
    12661 aatggatggt caaataattt cctcaatgcc gcctttacct cggcattaat gtttggttat
    12721 ttcatcggct ctcttaccgg tgggtttatt ggtgactact ttgggcggcg cagggcgttt
    12781 cgcataaatc ttctcatcgt cggtattgcg gcaacagggg ccgcttttgt ccctgatatg
    12841 tactggctca tcttctttcg cttcctgatg ggaacaggaa tgggggcgct gattatggtt
    12901 ggctatgcct catttacgga gtttatcccc gcgacggtgc gtggaaaatg gtccgcgcgg
    12961 ctctcatttg ttggaaactg gtcgcccatg ctgtctgcgg cgataggcgt ggtggttatc
    13021 gcttttttta gttggcggat aatgtttctg ctgggcggta ttggcatact gttagcctgg
    13081 tttctctcag gtaaatactt tattgagtcg ccacgatggt tggcagggaa agggcgaatc
    13141 gcaggtgcag aaagccaact tcgtgaagta gagcagcaaa ttgaaagaga gaagagtatt
    13201 cgtttacccc cgcttacttt gaaccagagc aacagcaagg ttaaggtaat caagggtacc
    13261 ttctggctcc tgtttaaagg ggaaatgtta cgacgtacat tagtcgcgat tactgtttta
    13321 attgcaatga acatttcgct ttataccatc accgtatgga tgccgaccat atttgttaac
    13381 tccggcattg atgtcgataa atcaatatta atgaccgctg ttattatgat tggcgctccg
    13441 gtagggatat ttattgcggc attaattatt gatcatttcc ctcgtcgatt atttggttcc
    13501 gccttactta ttattattgc cgtgttaggc tatatctatt caattcagac aacagagtgg
    13561 gcgattttaa tctatggcct ggtgatgatc ttctttttat acatgtatgt ttgcttcgcg
    13621 tcggcggttt atatcccgga actttggcca acgcatttac gcctgcgtgg ttcgggtttc
    13681 gttaatgccg taggacggat cgtcgcagtc ttcacgccct atggcgttgc ggcattatta
    13741 acacattatg ggtcgatcac ggtgtttatg gtgcttggtg tcatgttagt gctctgtgcg
    13801 ctggttctct ccatttttgg catcgaaacg cggaaggtgt cgctggaaga gatttctgag
    13861 gtgaattaaa tctttcgttg atggcgtcaa ttaccagatg tatcactgat gtgttttatc
    13921 tggcctacaa tgccagtaca gaattgtagg ccagcgtaaa attactcctg cgtttcttcc
    13981 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tttccataaa cgcgtgcgca
    14041 ctgtcctgtg gctggatatt tacgccacga caaccatcgg taatcacgtt caccttatag
    14101 cctaactgta aagcgtccag cacggtaaac ttaacgcaat agtcagtagc cagacccata
    14161 acgatcaatt cattgattac atgagcgcgc aaccagtcat cgagcgcagt tttctgccga
    14221 cgaccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
    14281 accgctgcga tcgctttttg cttcagtaac ggatgtaatt gtgcgccttc actgttctgc
    14341 acacagtgat cgggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
    14401 tctacaccgt gctgactggc aaaactgccg tgattcgccg gatgccagtc ctgactggcg
    14461 ataactgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
    14521 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
    14581 agtaacaggg cgcgaggggg cattgcagtc tccttaatca tccggcgtca gttcgccacg
    14641 caggttttgg ctcatggcct tgcgaatggt ttcagtatca agtttctggc taagcaggta
    14701 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
    14761 ggcgagggcg ttaccggtgg cataaccacc catgttcact ttaccggaca tacattgtgt
    14821 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
    14881 gttttgtggc gcattaccca cgccatatga gcgcagaatc aatgctttca caggttggcg
    14941 cagaaaattg cgcacgacgt cagcagaaat ccccggataa atcgtcacta cgccaattgg
    15001 ttgcggcgtg attggatgaa cgatcaattc cccttcgccg tgcggggcgg gtggcgtatt
    15061 caaacgacga atatggatac ctgcttccag taacggagga aggtttggag aggcaaacgc
    15121 atcaaaacca tcggcatggg ctttggtggt tcggttgcca cgatacagtc gattattaaa
    15181 aaagagagtt acttcgttga taggataatt cgccgcaacg tacaacgcat tcagcagatt
    15241 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
    15301 tttactgaga ttctcgagca tgaacgacag tgcagaggcg gtatacgcca tcgtgtctgt
    15361 gccgtgcaga atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
    15421 atgctgccag tcttctggcg tcatatcaga agaatccatc aatggcgtat attcatgaat
    15481 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgctgtag
    15541 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
    15601 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
    15661 aaagtatata cgtgaggggc agcgagcgca cctggatact ctgtggatat gagaaaagtc
    15721 cggcagaacg gagggattag tgtcccgccg gagaaaagtg gttaatcctg caacgcatag
    15781 gttacggtgg tgactacgcg taccgtttta ttaatcgccg taggatcatc ttcatcactg
    15841 ccactggcga agatctggaa cacgccttgt gaggcatcgc taattgaccc cacctggcta
    15901 cctgagtccg ttgcaaattg taatgctgcg ttgcgtgcat ctttggtggc ggcggcaatc
    15961 atctcagcgc gcagtgcgcc aataccatta aagcgatagg cgactttgct ctcagcgata
    16021 gcgccctccg tagcgacttg cagttcgttt aatttcgcga cgactttttc gacagcagca
    16081 acattatggg tttttatcgt cactgacccg tctgcgttat agcgtggcat tgtgggatca
    16141 tctttcagat aaaaggaatc tgttcgcttg cttgtccatt ggcccagggt tatctctttc
    16201 tcgttaatcc cctgagcatt gagttcggca acaatttttt tctgcgcgtc ttccagtttc
    16261 gggaacagtt cgccgggaat attaccctta aagttaatcg caattgttaa ttctgccgta
    16321 tctgcgcgaa cttctttttc agaaagccca cgcacattta cataacggtt attggttttt
    16381 aaatgtttaa caccatcgcc aacaaaataa ccacaggcgc taatccccag tccgaggacg
    16441 atagcggcaa ttaaagaggg ggccttattc actgatgact ccatatcaga tgctaaatga
    16501 gcaagggaat gtagtggaga gagggagtga aagacagtcc gattcaggag tgtggccgac
    16561 agcatatcgg ccacattttt gcaggactta acgcacgttg gcgcaggtca ggcaaaacgc
    16621 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    16681 ggctaccgag gccagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    16741 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    16801 ttccagatgc cactgtttca cttttgccag ctctgctgct ttggcgaccg catcatcgaa
    16861 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    16921 ctgggcgatt ttatcaatct gctccggcgt tgaatgacgc gcatcagcaa ccagcgtgat
    16981 aaagcgttta tagccattct caatgcttaa ctgcatcatc tgctgcactt ccggcggcag
    17041 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    17101 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    17161 cagggtgctg gggttagcca caatgtaatt agctggtgtg gaaatccagt aaccaccaga
    17221 tgccgccatg ccacccatcg atacaaccac aggcttaccc gctgcccggg ctgctgccag
    17281 ttcagcgcga atcacttcag acgcggtaac gctgccgccg ggactattaa cacgcagaac
    17341 aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc
    17401 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    17461 gatactgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    17521 ataattttta tcagttttac tccagccgaa ctctttggtc agagtttttt cgatttcggc
    17581 actcgatgcc agtgcatcga ccagcttgtt ttccagcgca tatttcgcgg tatcgccacc
    17641 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    17701 ctggcggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    17761 gtcagcttcg cgggctgccg gcgacatatc atcacgaata aacggttcaa cggcagattt
    17821 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagta acgatttgta
    17881 gtacagaccg ttggtggcaa agccgtgcag atcaaccacg ccttgcggag acagccaaat
    17941 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    18001 aaccggtttc ccgctgtcac gaaactcttt cagagctttg ccgatgtact gcatagacgg
    18061 ctggtcgccg cctgcgaagt ttttcagatc catcacaata ccggtgatat tgcggtcgtc
    18121 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgaa gacgatcgga
    18181 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt cgggtttatc
    18241 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    18301 accaccactg acctgcatcc agatccccac accaaccagc acgagaaaaa taaagaacag
    18361 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    18421 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtttcaggc
    18481 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    18541 ggctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcatga caggagttaa
    18601 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc
    18661 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    18721 taagtccatg caaccgtggc atttctttgt gattgaaggg gaagggcgcg agcgtttcag
    18781 cgccgtactg gagcaggggg cgattgctgc cggtagtgat aacaaagcaa tcgacaaagc
    18841 gcgtaatgcg ccgttccgcg caccgctcat cataacggtg gtggcgaaat gtgaagaaaa
    18901 tcataaagtc ccgcgctggg aacaggaaat gtctgcggga tgcgcggtca tggcgatgca
    18961 aatggcagca gttgcccagg ggtttggcgg catctggcgc agtggtgcat taactgaaag
    19021 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    19081 cctcggtacg ccgcagttga aagcatctac gtcgataaac gtcccggacc cgacgccgtt
    19141 tgtgacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    19201 ttcagacact ctcacttatc acttcatgga atgagggtta ccatacccgg attgcattga
    19261 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggcgctggt
    19321 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    19381 tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    19441 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    19501 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt tgctatgggc
    19561 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    19621 attgcccgcg aagtgaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    19681 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    19741 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    19801 acgaaaccgc tggggattgg cgttcttacc acggctgaga aaaaatcact gctgaaacca
    19861 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgtgtccttt
    19921 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gttttggttt actggggcat
    19981 ttaagcgaaa tgtgtcaggg cgcaggtgtg caggcgcgcg tcgactatga cgcgatcccg
    20041 aaattgccgg gggttgaaga gtacattaag ttgggcgcag tgcctggcgg caccgaacgt
    20101 aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg
    20161 tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    20221 gtcaaagcta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    20281 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattca atgcggttgt ttattgccga
    20341 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgcct aaaccgcacc ggaaaggcga
    20401 tggctttatc gagtgcggta atggtcaggt ggtgacctgg tgtatcggtc acctgcttga
    20461 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    20521 aattgtcccg gaaaagtggc aattacagcc ccgaccctcc gtgactaaac aacttaatgt
    20581 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggagacc cggatcgcga
    20641 agggcaactg ctggtggatg aagtgctgga ctacctgcaa ctggcaccgg aaaagcgcca
    20701 gcaggtacag cgttgtttga taaacgactt gaacccgcag gcggttgagc gggcgatcga
    20761 tcgtcttcgc tccaatagcg agtttgtacc gctgtgcgtt tctgcactgg cgcgggcgcg
    20821 ggctgactgg ctgtacggca ttaatatgac ccgtgcgtat accattctcg gccgcaatgc
    20881 cggttatcag ggtgtgcttt ccgtgggacg cgtgcagacg cccgtactcg gactggtggt
    20941 gcgccgcgac gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    21001 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    21061 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    21121 ccgtattagt ggtcaaccag ctattgtcac cagctataac gataaacggg aatcagaatc
    21181 cgcgccgctg cctttttcgc tttcggcgtt gcagattgaa gcggctaaac gttttggtct
    21241 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgtaca agctaatcac
    21301 ttatccgcgt tccgattgcc gctatttgcc agaagaacat tttgccggac gccacgcggt
    21361 gatgaatgct atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga
    21421 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcattccgac
    21481 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    21541 ccgtcagtac ttgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    21601 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    21661 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccgc tgcctgtggt
    21721 ggcgaaaggc gatgaattgt tgtgcgaaaa aggcgaagtg gtagagcggc aaacccagcc
    21781 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    21841 gcaggacaaa gatctgaaaa agatccttcg tgcgaccgat ggtctgggga cagaagcaac
    21901 gcgtgccggg attattgaac tgttgttcaa gcggggtttc ctgaccaaaa aagggcgcta
    21961 tatccactcc accgacgccg gaaaagcgct attccattcg ctgccagaaa tggcgacgcg
    22021 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    22081 ctatcaggac tttatgcagc cgctggtggg gacattgtac cagttgattg atcaggcaaa
    22141 gcgcacgtcg gtaaggcagt ttcggggaat aatggctccc ggcggtggag aagggaagaa
    22201 aaaggattcg ccacgcaaga gagcgccgaa aaaaagcccg ccatcagaag aggcgggcaa
    22261 tggagtgata atataaaggt tctttcaacc atctgatact gaagattttt atggccggat
    22321 aaggcgtttg cgctgtatcc ggcacaattg tcttaaatca cgccctggct taacatcgca
    22381 tccgcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt ttgctcacct
    22441 tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg caaacgtgcg
    22501 tcaactttct cggctttcca gcccaggcgc gcagcgtttt gcgccatttc caggcccgat
    22561 gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc tgcctgctgg
    22621 aacagttcag tcgcttcgat ggtggtcggc atatttgccc cttcggcgac ggctttaacg
    22681 ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt ggcgcaaggc
    22741 agggcgatat caaccggtac agaccacggt tgttggcctt cgagatagac cagaccaaat
    22801 tctttggcgt aatctgccac tcgaccatcg cggctggctt tgatttcgat aaggcgcgcc
    22861 agtttctctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc tgacgcagtg
    22921 atcactcgag caccaaattc catcgctttt tcgatagcgt actgggcgac gttgccggag
    22981 ccagaaacgg aaacgcgcat cccttcaaaa cccataccgt ggcgttttag cattgcttct
    23041 gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactgcc gccaaatgaa
    23101 aggcccttac cggtgaagac gcaggcggta ttgttggaga gctttttcat catccccgcc
    23161 ataaagccga cttcacgacc accaacgccg atatcacctg ccggaacgtc agtatccgcg
    23221 cccaggtggc gatacagttc agtcatcagc gcctggcaga aacgcataac ttcgccttcg
    23281 cttttccctt tcggatcaaa gtcgctgccg cctttaccac cacccatcgg cagagtagtc
    23341 agggcatttt taaaggtttg ctcgaagccg aggaatttga gaatggaaag gtttactgaa
    23401 gggtggaagc gcataccgcc tttgtacgga ccgatggcgg agctgaattg cacacgccat
    23461 gcacggttga cctgcacctg gttgcgatca tcaacccata ctacgcgaaa ttggatcacg
    23521 cgctccggtt caaccagacg ctccagtaat gacatctgac gatatttggg attttgttca
    23581 agaaaaggcc agagtgtggt cattacttca cgaacggctt gcgcgaactc ggtttgattc
    23641 gggtcgcgct tttggacatg gttgaggaat gactccagag aatatgtctg atccatagat
    23701 ataaaaccct tatatattaa tacgattatg cttatgttgt ttatgtcatg tgcttttgca
    23761 gttttcgact ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac ccaggaaagc
    23821 aaattaataa cgagagtaat ctcataacca aatgcgataa ctcagacgct aaagttttgc
    23881 cgcccggcta ccgttatctg tagaagagac aataacgtta aggagttccc tgtgagtcga
    23941 gcattgatcg ccgtagtatt agcatttcca ttaattacac tggctaaccc gcattatcat
    24001 ccggacgtgg aggtaaacgt accgccagaa gtgttcagtt caggcgggca gtcagcgcag
    24061 ccctgtactc agtgctgcgt ttatcaggat caaaattatt ctgaaggggc agtaattaaa
    24121 gccgaaggaa tactgctgca atgccaacgg gatgacaaaa ccctaagcac taatccgctg
    24181 gtctggcggc gcgtgaagcc ataaacgcct ctaataatgg aatgtcagca ggggccagcg
    24241 gatatcgcag cgcctcttca ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta
    24301 acgtcccgtg gaagtcgggt acgtgccagg catgaagatg gataatccgc cccgaaactt
    24361 ctcgctgatg gctggcaaca tagtcaccaa cagttgcttc gatgcccagt tcttcgttta
    24421 actcacgcac cagcgcctgc cgctggcttt catccggctc gattttacca ccggcaaact
    24481 cccataatcc cgcctgatcg ctctgggcag ggcgttgcgc gagtaaaatt ttgccatcac
    24541 gttcgatgat ggcggcaaca acttcaatca ttttcataaa ttacacacgg gcagataagc
    24601 gaaaaatgcc ataatatccg cactgttgct caatcagaaa ggctttatat ttaaggagtg
    24661 cctgtgctag accgccatct tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg
    24721 gttcttgata aaccgggcat tacgccagat ggtttaacat tagtgggatt tgccatcggc
    24781 gtgctggcac tgccttttct ggcgctgggc tggtatctgg cggcactagt cgccattttg
    24841 ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc ggcgcagagg gcttaccgac
    24901 gcgggcggct ttctcgatat ttctctcgat tttctttttt acgcgctggt gccttttggg
    24961 tttatcctcg ccgcaccgga gcaaaacgcc ctggcaggcg gctggctgct gtttgcgttt
    25021 atcggcacgg gcagcagttt tctcgctttt gctgcgctgg cggcgaaaca tcagattgat
    25081 aatcctggtt atgcgcataa atcgttttat tatctgggcg gcttaaccga aggcagcgag
    25141 acgattttgc tgtttgttct gagttgcctc tttccggcct gttttccgtg gtttgcgtgg
    25201 gtatttggtg cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg gtatctgacg
    25261 ctgaagtcgc tccagcgtca gtaattgcaa tgttatttac tgctgtccgg gccgcgttca
    25321 ccggttgcta ccggattttt tggatcgctg ctccattcgt accagccacc gtcgtaaacg
    25381 gagacgttat tccagcccat tgcgcgtgcg tacataaagg tttctgacgc gcgccagcca
    25441 gtgccgcagt agaatgaaac ttgctgatct ggtttgatat tccatgcttt ccacatagcg
    25501 gttatatcat cggcgctgcg catggtgcca tccgggttat ggaaatcttc catatgcgtc
    25561 gagtcgctac ctgcgtgtcc ccaacgtgct ccggctattt caccttttgg tttaatatag
    25621 ctgtaaccgc tggtcgtacc gataaattct ggccacgaac gaatgctcac cagcgatgca
    25681 tcctggcgat gcagcagtcc acgcgcttgt tccatatcaa gcatcagttg cggttgtgcc
    25741 gggatcttca cgccgaaatc cggttccgct ttcactttcg gtggcgtacc gcgctcaacg
    25801 ggcagacccg cgtcggacca ggtttgccag cctccgtcca gcaggcgcac atctttcacg
    25861 ccggcataaa gcataatttg cgccacacgc gctgcagcgt atacgtcacg cccgtacaga
    25921 atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca tcgctttcag ttgttcatca
    25981 gaaactttgt tccacagcgg ttcgctttcc acttcgttgg tgtcgatgta gtcagcgccg
    26041 ggaatatggc tgataaggta aaattttgga gcgccccagg ccgcttcaat gactttccag
    26101 tcaccggcag gtttcgccgt aacctcttta ccttgttgca ggtcatgcag ccactgcgga
    26161 tataccagct gttcaaagtg cggcagtttt tgcagacggg aaggttcgct tagcgcgtca
    26221 ctcaggatgg agatatgcgt aaaacctgct ttttgcagtc gcgttttgac ggcgtcgaca
    26281 tctttgtcat taccgtacag tgccaccggg gcatcggctt tcaggttatg ttgcttgatc
    26341 cactcgttga gctgttcggt gctcattttg tcgagccagc tagcagagag gtttaaggcg
    26401 gcaggttcat gaccggaagg gccatttaag gtttgtggcc agccgttata aaacgcgctg
    26461 gggcgagtat ctatcgcttt gccattttgt tgttgaagct ggtcaagtgt aagaggcttc
    26521 gccagttcag cggcccacga agaagcgcaa gccagcccta aagccattgc cagcgcggtc
    26581 atttgagaaa cacgtttcat cgaaaatccc gacattgtag ttttgagagc gccattctga
    26641 actgacgcat cgggaaaaac tttgcgttat cgcagtttgt tgtaattttc tgaccactgc
    26701 gccatatcca gaacggggct atcaggagga acatcctgaa gatcgtgcgt cacctgcaca
    26761 acagggatcg ccatggcgcg aatttcgctg aacacccaat ggcgaaaatt atcgcgcagg
    26821 gccacatcaa gacggctgaa tggctcatcc agtagcaacg cttttggctg agcgagaagg
    26881 gcgcgaagca gagcaacgcg cgctcgctga ccgccagaca aagtggcagg atcctgatgg
    26941 aacgctccgt caaggcctga acgctcaagt gcatcattca cggcattacg tcgggcattc
    27001 cccttaagtg tcgccggtag cgccagcagt aaattttgcc cgacgctgaa ctggtcaaat
    27061 aacagtgcat cctggaaaag aatgccaatc tgacgctgtg cggtgggtag catgtcaatc
    27121 cgttgctcat taagccatag ctcccctgta caagaaaact gtccggccag cgcaccaatc
    27181 atccacgaaa acagagtgga tttcccgcag ccggatggtc ccattaacgt gacaatgtca
    27241 cctttatcta ctgtaaagtt aacgtttgtc agcaagcggc tttctggtaa acgtagcgaa
    27301 acatttttca cgcagagcat tagcggagtc cttgtctgac ataacctacc cattttacga
    27361 ctaacgcggt cagggcaaaa ataataagcg gtaacagcag ttgccataaa gcctgggcgg
    27421 cgagaatacc gttgctgccg ccgctgctta atgccaccgc ttcagtggtg agcgtcggaa
    27481 aacgccccgc gcccagccac agcgtcggca tatactgcgc aatactgact gaaaatccca
    27541 ccgcgaaggc aatcagcgca gggcgcaaca tgagtgggca ttttacgtaa aagaagattt
    27601 tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca atgcgctgcc
    27661 aggcgggttg caggataaac agcatccacg gcattaccca cagcagatgc ccccagacca
    27721 ccgccgtcca gcttccatcc aggttcagcc atagcgccag cgtgtactgg cccgccacca
    27781 gcggcagagc gggtaataaa atgggcaacc atagccacaa ctggcgacgc tgtggccccc
    27841 attccagcca cagcagtaac aagagcaaag cgatgaatgc cgccaccagc cccattgtca
    27901 ggctgttgat gagcgcttga ctattgatcg tcgactgatc cgcgaggatc gccagcagaa
    27961 ccacacacag cacaccggtt aagggtaaaa aactcgccag cgtattgctt ggcaataaag
    28021 gcgtggcagg cttacgaacg ccatctacgc ggggaatagt acgccgccag ccacgccata
    28081 gcagatagcc cagcaaaacg taggcggcga gtaacagcat cagcagcagg ctggcgagtg
    28141 cgcctttcgt ttgttgatcg gcgtcgccct gagttaacca ctgccagcta attaccgcca
    28201 gcgtcggggg attacctggc ccaagaataa tcgccacatc cacgaccgac agcgaccagg
    28261 cgacaatcgc cagcatcgcc attgctaacg cagacgcgac ggagggcaac agcagccagt
    28321 tcaggcattg ccagcggctg tagcccagcg aatccagcac aatgagttgc tgcaacagcc
    28381 gtttttcgct caatactgcc gctaagatcc acagcagaaa tgcgctttct ttcaccgcca
    28441 gggtgaggcc cagcccaata cccaatttgt ccattggcgg agtgaaatac gggaaatagt
    28501 cataaagcag ccctccgtca gcaaagagca gaagggcgct ggtggcaaaa gccacatggg
    28561 gaatggcgag cagccacggc agacgggcgc acatacgctg ccatttcggc cctggccaca
    28621 acgccacaat caccagcagg gcaatcaaca acgccccgac cgccgcaatg gttgtcgaca
    28681 ccagcgtcgc cagtaatgcc tgcggtaact gtggatcggc aaataacgcc tgccagtgcg
    28741 tcaacgacaa agcaggcgat atcagcgtga gagctgccgg gatcagcggt gcataaatca
    28801 ccgctatcat cgcccacagc aaaaagatta atgcgtaccg taacggtgta gccattcttg
    28861 ttccagcgca tttacccaac ctgcgtgcgg ttcagccagt accggcggca gatcctgcgg
    28921 cattctcgat tgcaatgttt cgcgctgccc gtcaggcagt ttttgcgggt cgagaacaga
    28981 aggatcgccc cagacagcag gatctgcttt acgcagttgc gcatcgggtg agagcaggaa
    29041 attggcaact accttcgccg cagcactggc attcgcgttg gcaggaatgg tgacgaaatg
    29101 gacgttgcca atcatcccct cgcgaaaacc aaaactgtaa ctgcttgcag gcaaatcgcc
    29161 gctggcgatt ttttgctgcg catgcgcggg gttaaaggtc agcgacaggc gcaatgtgtt
    29221 agctttcagc agagcatcca tccgcgcggg cgaaggcggg aaatcctttc cttcgcgcca
    29281 caaatacgga tgcagtgcat caagatattg ccacaagggc gcagtgacgc gggcgaaagt
    29341 ggcatcgtcc ggcgcttctt ttaatgctgt gggatctggc gttagcataa tcagcaactg
    29401 ttcaagaaac gccgtgccgg taaagtccgg tgggcgcgga taggtaaccg tgccgggatt
    29461 ggctttagca aactccagta aggcttgcgg cgtttgtggt ggctgtagcg taacatcgcg
    29521 gcgggcgata aacgtcagtt gtgcgccgcc ccagggcgat tcagcccctt ctgtaggcac
    29581 tgaaaaatct tcccgcaccg gaagctgtgt gtcgacatag cgccagttgg gcagagtctc
    29641 cgcccagccg gtttgtaata aattggcctc ttttaaggtg cggaagtttt cgccgttcac
    29701 ccagagcaga tccaccgaac cgcccgtttt acgtccggca gcaacttcgg tctgaatgcg
    29761 tttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag cgtaatgggt
    29821 tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc cgccccaggc
    29881 gttaaaccag acggtttgtc ctttggcctc attttttatt tcctgccagt cactggcatg
    29941 tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc gcatcttctc
    30001 tccttaagaa cgtttttcag gaatacggca cagcccctga gcgggaatgt ccatggcaga
    30061 attaaaacgg gctgacagat tttgcaggcc tatcaatgcc gtcagttcgg tgagcgcctg
    30121 agcgtcaaaa tgcgtagcca gtcgggcgcg cagagcatca tcgaccgttg gcggcgttac
    30181 gcttgcggct tcggcgtact ccagcgccag ccgttcttca tcgctaaaga gcgggctttg
    30241 ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg cgactttcat
    30301 actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg cgctgacaag
    30361 cgatcgtact accggatcga gcggtgagcg tttgcgctcc agccagccga caaacatcga
    30421 caccagataa aagataaacg ggatccgccc ccaccagcga atcgggctta gcacctcgcc
    30481 gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt tgctaagcgg
    30541 cggtaaaccc atcaggcatt cctcttctgg cgctcaagcc atattttctt cgccataaaa
    30601 atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa agtaccgcct
    30661 gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc aagcccggtg
    30721 gcgataaaaa acgagcgaaa acgtattgaa gtcaaaccgg cagcatagct gattggatcg
    30781 aaagggacaa aaggcaataa ccgacatacc aggatggtgt gtttgccgta gcgagtgaaa
    30841 aagccgtcca tactgtcaag tacggttttg ccggttaatt tttccaccac ttcgcggccc
    30901 atcactctgg cgataaaaaa gcacagcgcc gcgccggcca tcgaactggt ccacgacagt
    30961 aagccgcccc agaacgcgcc aaacagcgac gcattggcaa aggtgatcaa aaacgcaggt
    31021 agcggtgcag caatggcctg taaaatcatc aacaagaacg agacaactgc cgctagtgcg
    31081 ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa cgccgcaacg
    31141 ctgcgattga taaactcatg gacgccggga agcagcgccc atgccagcag cacagcgaac
    31201 aacaggatga tgagggttat acggtagtac cagattttac gcgattgcat cttcatcatc
    31261 ctttatcctg ctttattcgg tgattttaaa ggtaaccgtt gccagcgtgt tatccgccgg
    31321 gagaactgag gcattgcctt tgaatttcac ttcgccgcgt ttttccaccg caccataagt
    31381 gtatgttgca ttgctgacga tgccgaccgg gcagctatcc agacacacca ggcaacccgt
    31441 tttcttctct tctgctgccg ttaaattacc gccaaagcgc atgtccagtt tcttgccatt
    31501 actgtcaaca atcacttcat cgaaggaata cgcttttgcc gccccttgcc agttgaccga
    31561 aatatccagt ttgctgcctg tgacatgagt cgtttcttta ttatccattg tcatgttttc
    31621 gcccggcgta ccacctgcct ctttcagggc ttcataaaat gctttcgggg tcgcataacc
    31681 cataaacagc gatttatgtc cgttggagcc atctttaaac acaataccgt gacgagtgtc
    31741 gtcggtgagg aaacgaccat taatttgcac cagcatggtt accgtatggt tattggcatc
    31801 gacacgcagc ggttggctgt tcgaaagtcc gtcgtattcc acttttgcag ccgcattctc
    31861 tttttgatcg cagccgctaa gcccagcaac agccagtaag cagagtgcag ccagtccttt
    31921 tttccatgac actgaataat gttgcaacat cgttctatcc ttcatttttt gggtgagaaa
    31981 gtgggctgcg gcgagaagca gacagatcca catgtttgtg tcgggcgtag agttttgcga
    32041 gctggacgag aataaacagt gccaggcccg ccagacagag ttgtaaaata aagcgcagcg
    32101 taatgccttc attggcgaga tcgcttgcca tcacggtata aataacaata ccgggtaggg
    32161 tcgtgagtgc cgaaataagg gtataaggcc agaaggcgat tgtggttaat ccgtaagcgt
    32221 aattttgaat attgtaagga aacaacggga ttaagcgggt cagaataaga aaatcaatac
    32281 cgttacgcgc aatgcctttt tcaatggcct gaaaggtatg gctatggcca acgtatttca
    32341 gcagtaaatc acgtcccagc cagcgcgcca gaaggaatga gcacgaggag gccagcgtgg
    32401 cggcaattaa tgatagtagt gtccctaaga gcgggccaaa aactattcct cccgcgatca
    32461 ccagtatgct tcctggtaac agaaagaggg tggcaatgat gaataacaga atatagaggc
    32521 tatagccgaa caatccgctc tggcgaatga gtgtctgtaa gtggggtaaa tcggtgagca
    32581 gatcgaataa accgaaagcg tggatagcgt aaatgaccag cgcaaaaata agacaggcaa
    32641 aaagaaattt acgctcagcg ttcatcgtgg agtttcctat atcaaacttg ataatagaga
    32701 agtggtcaga caactaaaaa cgcagtgacg gtataacaaa ggacggcggg caacaaatca
    32761 ttactgacag tttgttgatt tttctcaagg ttaattctcc tgacccagtt tgagccagga
    32821 gatcagctaa attagcggcg gaaggtcgcc cagacggggg cgtgatcgga cggtttttcc
    32881 atgctgcgaa tttcatagtc gatgccggtt tctacgcaac attctgccag cggttggctg
    32941 gcgagcagca ggtcgatgcg cagaccgcga ttatcgtcaa aaccttttga gcggtaatca
    33001 aaccatgaga aacgatctgc tgtttgcgga ttcgcatggc ggaaggtatc gaccaacccc
    33061 cagctcatca gcctctccat ccattcgcgt tcctccggaa ggaaagaaca ttttccggta
    33121 cgcagccagc gcttacggtt ctcttcgcca atgccaatat ccaggtcgcc aggactgata
    33181 ttcatatcgc ccataatcag caccggattt tcacgcttga gttcggtttc caggtagttt
    33241 tgcagattct gataaaactg cgcctttgcc gggaatttta tcggatggtc gcggctttca
    33301 ccctgcggga agtaaccgtt gatcacggtg atattgccca gcggcgaggg gatttccgcc
    33361 ataataatcc gccgttgcgc ctcttcgtcg tcaccgggaa agccgcgacg cacggaaatc
    33421 ggcgtctctt tggtcagcag cgccacgcca taatggcctt tctgcccgtg ataaaacacg
    33481 ttgtagccga gcttcgccac ctcttcgagc ggaaacatat cgtcatgaac ttttgtctcc
    33541 tgcaggccaa tcacatccgg ttggtgcttt tcgacgatgg cttcaagctg gtgaggtctg
    33601 gcgcgcaggc cgttgatatt aaaagagaca aatttcatag tcgctgccat ttattcagat
    33661 aaagagtgcg tggatggtag cagaatttcg ctttgcttac cgcctgttgt taagtgattt
    33721 gatcgatggt tgttaatgat gtcaacgatg gcgcaaaaaa tgcccgcttt tggtgcgcgt
    33781 tgcgtcagaa tggcgcagta atttccagta aatttcgtca atgatcacaa ttccagaatt
    33841 atattttact tctgcataac attgcgtttt ttattctttt tattcaccca catcgcaaac
    33901 gtattcactt tatatgcact ttaaatgcat atgatgtggt tataactgct tgatttccag
    33961 ttagcctccg ccgtttatgc acttttatca ctggctggca cgaaccctgc aatctac
//
LOCUS       DAPQZD010000039        33463 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPQZD010000039 DAPQZD010000000
VERSION     DAPQZD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.039"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            142..477
                     /locus_tag="RO454_001782"
     CDS             142..477
                     /locus_tag="RO454_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDY0933627.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            570..653
                     /locus_tag="RO454_001783"
     CDS             570..653
                     /locus_tag="RO454_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933628.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            753..1574
                     /locus_tag="RO454_001784"
     CDS             753..1574
                     /locus_tag="RO454_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDY0933629.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1613..1942)
                     /gene="mdtI"
                     /locus_tag="RO454_001785"
     CDS             complement(1613..1942)
                     /gene="mdtI"
                     /locus_tag="RO454_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDY0933630.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1929..2294)
                     /gene="mdtJ"
                     /locus_tag="RO454_001786"
     CDS             complement(1929..2294)
                     /gene="mdtJ"
                     /locus_tag="RO454_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDY0933631.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2706..3740
                     /gene="tqsA"
                     /locus_tag="RO454_001787"
     CDS             2706..3740
                     /gene="tqsA"
                     /locus_tag="RO454_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDY0933632.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3765..5153)
                     /gene="pntB"
                     /locus_tag="RO454_001788"
     CDS             complement(3765..5153)
                     /gene="pntB"
                     /locus_tag="RO454_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDY0933633.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5164..6696)
                     /gene="pntA"
                     /locus_tag="RO454_001789"
     CDS             complement(5164..6696)
                     /gene="pntA"
                     /locus_tag="RO454_001789"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDY0933634.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7220..8164
                     /locus_tag="RO454_001790"
     CDS             7220..8164
                     /locus_tag="RO454_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0933635.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8350..9732
                     /locus_tag="RO454_001791"
     CDS             8350..9732
                     /locus_tag="RO454_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDY0933636.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9769..10491
                     /gene="folM"
                     /locus_tag="RO454_001792"
     CDS             9769..10491
                     /gene="folM"
                     /locus_tag="RO454_001792"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDY0933637.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(10488..10823)
                     /locus_tag="RO454_001793"
     CDS             complement(10488..10823)
                     /locus_tag="RO454_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDY0933638.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10952..11671
                     /gene="rstA"
                     /locus_tag="RO454_001794"
     CDS             10952..11671
                     /gene="rstA"
                     /locus_tag="RO454_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDY0933639.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11675..12976
                     /gene="rstB"
                     /locus_tag="RO454_001795"
     CDS             11675..12976
                     /gene="rstB"
                     /locus_tag="RO454_001795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDY0933640.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13052..13981
                     /gene="tus"
                     /locus_tag="RO454_001796"
     CDS             13052..13981
                     /gene="tus"
                     /locus_tag="RO454_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDY0933641.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13978..15381)
                     /gene="fumC"
                     /locus_tag="RO454_001797"
     CDS             complement(13978..15381)
                     /gene="fumC"
                     /locus_tag="RO454_001797"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDY0933642.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(15524..17170)
                     /gene="fumB"
                     /locus_tag="RO454_001798"
     CDS             complement(15524..17170)
                     /gene="fumB"
                     /locus_tag="RO454_001798"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDY0933643.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17369..18544
                     /gene="manA"
                     /locus_tag="RO454_001799"
     CDS             17369..18544
                     /gene="manA"
                     /locus_tag="RO454_001799"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDY0933644.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18645..20153
                     /locus_tag="RO454_001800"
     CDS             18645..20153
                     /locus_tag="RO454_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDY0933645.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(20198..21464)
                     /gene="uidC"
                     /locus_tag="RO454_001801"
                     /pseudo
     CDS             complement(20198..21464)
                     /gene="uidC"
                     /locus_tag="RO454_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(21503..22875)
                     /gene="uidB"
                     /locus_tag="RO454_001802"
                     /pseudo
     CDS             complement(21503..22875)
                     /gene="uidB"
                     /locus_tag="RO454_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(22872..24683)
                     /gene="uidA"
                     /locus_tag="RO454_001803"
     CDS             complement(22872..24683)
                     /gene="uidA"
                     /locus_tag="RO454_001803"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDY0933646.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25073..25663)
                     /gene="uidR"
                     /locus_tag="RO454_001804"
     CDS             complement(25073..25663)
                     /gene="uidR"
                     /locus_tag="RO454_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDY0933647.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25892..26659)
                     /gene="hdhA"
                     /locus_tag="RO454_001805"
     CDS             complement(25892..26659)
                     /gene="hdhA"
                     /locus_tag="RO454_001805"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDY0933648.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGDGVQELN"
     gene            complement(26771..27799)
                     /gene="malI"
                     /locus_tag="RO454_001806"
     CDS             complement(26771..27799)
                     /gene="malI"
                     /locus_tag="RO454_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDY0933649.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            27974..29566
                     /gene="malX"
                     /locus_tag="RO454_001807"
     CDS             27974..29566
                     /gene="malX"
                     /locus_tag="RO454_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDY0933650.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            29576..30748
                     /gene="malY"
                     /locus_tag="RO454_001808"
     CDS             29576..30748
                     /gene="malY"
                     /locus_tag="RO454_001808"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDY0933651.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            30852..31853
                     /gene="add"
                     /locus_tag="RO454_001809"
     CDS             30852..31853
                     /gene="add"
                     /locus_tag="RO454_001809"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDY0933652.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31889..32929)
                     /locus_tag="RO454_001810"
     CDS             complement(31889..32929)
                     /locus_tag="RO454_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDY0933653.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            33172..33297
                     /gene="blr"
                     /locus_tag="RO454_001811"
     CDS             33172..33297
                     /gene="blr"
                     /locus_tag="RO454_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDY0933654.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         8270 a         8691 c         8086 g         8416 t
ORIGIN      
        1 tccttaagtc acaatacgtt attatcaatg ctgtaattta ttcagcgttt gtacatatcg
       61 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      121 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      181 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ctgcctttgc tgcagagact
      241 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgcctgcgaa aaccacacat
      301 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      361 aaaaatgcca aagctgaaca gaaagctcct gaacaaaaag cgcaggcagc gaagaaacac
      421 gccaagaaac acagccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      481 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      541 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      601 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgatcgtagt
      661 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      721 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      781 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      841 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      901 cgacccaatc accgtgggat gcggttggcc aactggaaac ggccagcggc aatttatgta
      961 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1021 caaagggtaa agccgataaa gcggtagcgc tgcgtttcgt gtcaaataaa ggtctttggc
     1081 gctatgagat ccacgacata gaaggccgcg ttgatccaac actgggaaag cggttaaaag
     1141 cagatgggga tggctggatt gtacctcccg cagctgcgcc gtgggacttc ggtttgattg
     1201 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1261 cgcttaccgc cgcattaaaa gcggctggtc gtaaagtgac tcaggcgggc taccctgaag
     1321 atcatctcga tacgttgtac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1381 cggtgatgtc gcatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1441 ataccgatga cggctggcaa ttaattgggg tgcaaagctc ggctcctgcc gcgaaagatc
     1501 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1561 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtgtt catcaggcaa
     1621 gtttcaccat gatcattcca gccaacagca gcaccaggcc aatccagcct ttacgattta
     1681 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1741 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1801 cagccagcac cgccgccaga gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1861 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1921 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     1981 cctgacttga tcaacacaat cccggccacc agggtggtta acccggcaat tttcatcagc
     2041 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accaatacct
     2101 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2161 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2221 gcccatttca ttgacagcgt accggtaatt tccgtagcaa tagccaggcc taataaaatc
     2281 caataaatat acattgtcct tctcctgcaa gagaattatt ttaattttcg cttaattcag
     2341 cgaaaccaaa gtaaagtgat caagccccgc gcatagcgca agcttaggca gaagaaagga
     2401 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2461 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2521 agtgttgctt ctcgtcatcg cggatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2581 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2641 ttcgttatga tgaaggagcg aaaaagacat tattattagc aaaggaagaa aaaacgggga
     2701 caagcatggc aaagccgatc atcacgctca atggcctaaa aatcgtcatt atgttgggaa
     2761 tgctggtcat tattctctgc ggtatccgtt ttgccgccga gattatcgtg ccgtttattc
     2821 tcgcattatt tattgctgtt attcttaacc cgctggtgca acacatggtc cgctggcgtg
     2881 tgccgcgtgt actggcagtg tcgattttga tgaccatcat cgtgatggcg atggtgttgc
     2941 tgttagctta tctggggtcc gcgctcaatg agttgacgcg gacgttaccg caatatcgca
     3001 actctattat gacgccgctg caagcgattg aaccgttgtt gcaacgcgta gggattgatg
     3061 tctcagttga ccaactggcg cattacattg atccgaacgc ggcgatgacg ttgctcacca
     3121 acttattgac gcagttatct aatgccatgt catcaatctt tttattgctg ctgacggtgc
     3181 tgtttatgtt gctcgaagtg ccacaattgc ccggaaaatt tcagcaaatg atggcgcgtc
     3241 cggttgaagg gatggcggcg attcagcgcg cgattgacag cgtgtctcat tatctggtgc
     3301 tgaaaacagc catcagcatc atcaccggcc tggtcgcctg ggcgatgctc gccgcactcg
     3361 atgttcgctt cgcttttgtc tggggattgc tggcctttgc gcttaattac atccccaata
     3421 ttggttcagt tctcgcggca atccccccta tcgctcaggt actggtgttt aatggcttct
     3481 acgaagcgtt gctggtgctg gcgggatatc tgctgattaa tcttgtcttc ggcaatattc
     3541 tggagccgcg catcatgggg cgtgggctgg ggctttccac attggtggta tttttgtcgt
     3601 tgattttttg gggatggttg ttaggaccgg tgggtatgct gctttccgtg ccgttgacaa
     3661 ttattgtcaa aattgcgctt gaacaaacag cgggaggtca aagcatcgcc gttctgttaa
     3721 gcgatctcaa taaagagtga cggcctccgc agaggccgtc agggttacag agctttcagg
     3781 attgcatcca cgctggcttt ggcgtcacca aacagcatgt gggtgttttc cttgaagaac
     3841 agcgggtttt gcacaccagc atagccagtg ttcatcgaac gtttaaagac aatcacgttc
     3901 tgcgctttcc acacttccag cacaggcata ccagcaatcg gactcttcgg atcatcctgc
     3961 gccgccgggt taaccgtatc gttagcacca atcaccagta cggtatcggt atcagcaaag
     4021 tcatcgttga tctcgtccat ttccagcacg atgtcatacg gtacttttgc ttcagccagc
     4081 aatacgttca tatgtccagg caaacgcccc gcaaccgggt ggataccgaa acgcacgtta
     4141 ataccacgag cgcgcaattt ctcagtaatt tcagcgacag gatattgcgc ctgcgcaact
     4201 gccatgccgt accccggagt aatgatcact gaatgggaat ttttcagcag ttccgctgtc
     4261 tcttctgcgg tgatttcgcg gtgctcgccc acttcctgat catcaccagt agaagagccg
     4321 tcggtgccga aaccgcccgc aataacgctg ataaaggaac ggttcatcgc cttacacata
     4381 atgtaagaaa ggatagcccc cgaagaaccg accagcgcac cggtcacaat cagcaggtcg
     4441 ttgctgagca taaagcccgc agccgcagcc gcccagccgg agtacgagtt cagcatcgac
     4501 accaccactg gcatatctgc accaccgatg gaggcgacta aatgccagcc gaataccagc
     4561 gcaatggcgg tcattatcag caatgccagc acttgcaggc cgacgctatc cgtgcgaaca
     4621 aatacaatca gcagcaggaa ggaaacgatc agagccgcca ggttcatttt gtgacggttt
     4681 ggcagcatca acggtttaga cgaaatcttg ccacacagtt tgccgaacgc caccaccgaa
     4741 cccgtaaacg ttaccgcccc gatgaagata ccgaggaaca cttccgtcag gtgaatattg
     4801 accagaatcg gtgccattcc cgcgtcatga tgcagatagc tgttaaaacc aaccagcact
     4861 gccgccagac ccacgaagct atgcaggatc gccaccagtt ctggcatttc ggtcatttca
     4921 actttcttcg ccagacggat accaattgcc ccaccaataa ccatcgccag caagatccag
     4981 ccaacattac ccgtatccgg tccaaaaatg gttgcgatta acgcaatcgc cataccggcg
     5041 ataccgaaat tgttaccctg gcgagacgtt tcatgtttcg aaagcccggc cagactgaag
     5101 ataaacagga tcgcggcaac aatgtatgca gctgtaacta atcctccaga catatgttac
     5161 cccttaattt ttgcggaaca ttttcagcat gcgctgagtc acggtgaagc caccgaaaat
     5221 attaatgctg gctataagca ccgcgataaa actaaggaag ctaacccagc cgccctggcc
     5281 aatctgcaac agtgctccga caacaataat ccctgaaatc gcgttggtga ccgacatcaa
     5341 cggtgtatgc agcgcgtgcg atacattcca caccacgtaa taaccgacaa cgcaggccag
     5401 cgcgaacaca gtgaagtgtc caaggaactc ttttggcgca acgcttgcca tccagccgaa
     5461 aaggatgatt gccaacgcca tcaacgcgta tttacgccac ggtgagcagg cacatttttc
     5521 ctcagttttc acttccggtg ccgctttttg tgccgtctgc ggctgagctg atacctgaat
     5581 cggcggtgcc ggccaggtaa tttcgcccgc acggatcaca gtcacgccac gaatcaccac
     5641 atcatcaaaa tccacagtga tgttgccgtc tttctctttg cacaacagtt tcagcagatt
     5701 aacgaggtta gtgccgtaaa gctgtgagga ttgcgtcggc agacggcccg gaagatcggt
     5761 gtaaccaatt actttgacac cattttccgt agtgaagatt tcacccggca cggtgtattc
     5821 acagttgccg ccgttttggg ctgccaggtc gacaatcaca ctgcccgcct tcatggagtc
     5881 aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg cggtggtgac
     5941 aatgatatcg acctcttttg cctgggcggc aaagagttcc atttccgctt tgatgaacgc
     6001 gtccgacatc actttggcat agccatcgcc gctgcccgct tcctctttaa aatccagctc
     6061 gaggaattct gcgcccatac tttgaacttg ttctttcact tccgggcggg tgtcgaatgc
     6121 acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccagacctg cgacaccggc
     6181 accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtgattt gtccggtaaa
     6241 gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga tattcgccat
     6301 cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagagt ccatcgccat
     6361 cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg caggccagat
     6421 aaaactcacc agcgttgtcc caggattcag taacgcaatt tcatcatcta acggcgcatt
     6481 gaccttcaga atgatctctg actgccagac gctattccct tctacaattt cagcgcccgc
     6541 ttgcacaaac gccttatcgt caaaacttgc cagttgaccc gcgccgctct ctaccgcgac
     6601 ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa cacgggtttc
     6661 attggttaac cgttctcttg gtattccaat tcgcatgata ttcccttcca tcggttttat
     6721 tgatgatggt ttgcttgtgt caggagccac acaagccgct catgtacgag ctaaatgtta
     6781 ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta tgcctgtgat
     6841 ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat tcagccgtga
     6901 tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc ggcggccggg
     6961 taaaacgaga catcgccccg gcacaaatca ctacttaaca ttaaattaac ttatacaatt
     7021 cagttgcttc agtagtattg atggtgatac ggctgttttt taagcataga cggtcatttg
     7081 agcagaatta aaattggttt aaggaatgtg atatgaaaaa tgacgcagac agttacaccg
     7141 tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc tggtttgcgc
     7201 aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg cactgctttc
     7261 tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag cggcagcggt
     7321 taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg aagcggtgaa
     7381 agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg tcgacacttc
     7441 tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag ccgatgctga
     7501 aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt gtcgaactgc cgaaagatca
     7561 ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc gtagccagcc
     7621 agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa ggtgcctact ctttctacat
     7681 tgttcgtcaa atcgatgcca accagggcgg taaccagcgt attactgcat tcatctataa
     7741 aaaagatgct aagaaacgta tcgtccagag cccggatgta atcccggcag attccgaagc
     7801 aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga tcccgggtgt
     7861 tgcgactacc gcatcaccaa gttctgaagt cggtcgcttc tttgaaaccc agtcatcaaa
     7921 aggcgggcgt tacaccgtca cgctcccgga tggcactaaa gtcgaagaac tgaacaaagc
     7981 gacagcagcg atgatggtcc cgttcgacag catcaaattc tctggcaact acggcaacat
     8041 gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta agtattacca
     8101 catcacccgc cagtggcagg aacgtggtaa taacctgacc gtcagcgcag atctgtataa
     8161 ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt tttagtcata
     8221 aatttccctg cgaccattgc atgcagacac atctcttcgt aaaatcccgc gcttcagtgg
     8281 caaactccat ttttatgcgt tttgccctaa tatttattct tcttgtcacg ttttaatcac
     8341 tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt attaagctca
     8401 atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc cagcccggca
     8461 gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc ctttgccatg
     8521 ctgatcctca cgcgcattcg ccccgaactt gacggcggta tctttaccta tgcccgcgaa
     8581 gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg cgcggtcatc
     8641 gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac ggacacgccg
     8701 gaattgcgcc tgtttggcga tggtaatacc tggcaatcga tcgtcggtgc atcggcttta
     8761 ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag cattaacctt
     8821 gtggcgacac tggcaaaatt gttgccgttg ggtctgtttg ttgtgctggc gatgatgatg
     8881 ttcaaactgg ataccttcaa gctcgacttc accggacttg cacttggcgt acccgtgtgg
     8941 gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt ggaaggtgcc
     9001 gtggttgttt ctgcgcgtgc gcgtaataag cgtgatgttg gcaaagcgac actgctggcg
     9061 gttctctccg ctctgggcgt ttatttgctg gtaacgctgc tttcgctggg cgtggtggca
     9121 cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt ggaaatgatg
     9181 ggaccgtggg gcgaaatcat catcgctgcc ggtttgatcg tttccgtttg cggcgcgtac
     9241 ttaagctgga ccatcatggc agcagaagtt ccgttccttg cagccactca taaagcattc
     9301 ccgcgcattt ttgcgcgcca gaacgcacaa gctgcgccat ccgcttcact gtggctgacc
     9361 aacatctgcg tgcaaatttg tctggtgctc atctggctga ccggttccga ttacaacaca
     9421 ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg cgcgttcttg
     9481 ctgaaaatcg caacccgtcc actgcataag gcggtaggtg tcggtgcctg catttatggc
     9541 ttatggttac tgtacgcctc tggtccaatg cacttgctgt tatccgttgt tctgtatgcg
     9601 ccggggctgc tggttttcct gtacgcgcgt aaaacgcata cacatgataa tgtactgaac
     9661 cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc gacctggatg
     9721 ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat gggtaaaacc
     9781 cagtccttgc caatattaat tactggcgga ggtcgccgca tcggcctcgc cctcgcatgg
     9841 catttcatta atcaaaagca accggtgatt gtcagctatc ggacgcacta tcccgccatt
     9901 gatggactga ttaaggcagg tgcgcagtgt attcaggctg atttttcgac caacgacggt
     9961 gtgatggcgt ttgccgatga agtactaaaa agcacccatg gtctgcgtgc tattttgcat
    10021 aacgccagcg cgtggatggc ggaaaaaccg ggagcgccac tgaccgacgt actggcttgc
    10081 atgatgcaga tccacgttaa taccccatac ctgctcaacc atgctctgga aagattactg
    10141 cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt ggtggagcgc
    10201 ggtagcgaca aacatattgc ctatgctgca agcaaagcgg cactggataa tatgacccgc
    10261 tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgcgcc atcgctgatc
    10321 ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa atcactgatg
    10381 aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact taccagctgc
    10441 tttgtcaccg gacgcagtct cccacttgat ggcggtcgtc atctgcgtta atgcagctta
    10501 atccagcata taatcaacac ccatgcgctt attccccagc acgcaacaga gccaacaaaa
    10561 gcagcgggca agcgcatcat tccggtgaaa taccatagcg acgccaggta aacaaaatag
    10621 ggaataatcg accacatact aaaaatgatg gttgcgcgta aggcttcaat gccgcgttcg
    10681 ctggcaacaa tataatgcgc gataagcgca aaggtcggga aaagtggaat cagcccggcg
    10741 atataataat ttttcgtttt tgctaaaaca ccaatcaaca gcactaccag cgcaccgagc
    10801 gcggctttga ttaccagccc catcttttta ccttaacact tccataacaa gtcatcagta
    10861 gaatacctga tgaaaccttg tttagaaacg attgatagta agtaaaaaca gtgcggtgta
    10921 ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact atcgtatttg tggaagatga
    10981 tgcggaagtc ggtgcactga ttgccgcata cctggcaaaa catgatatgc aggttaccgt
    11041 agagccgcgc ggcgaccggg ccgaagaaac tattctgcga gaaaatccgg atttggtgtt
    11101 actcgacatc atgctaccag gcaaggacgg catgaccatt tgtcgtgatt tacgcgcaaa
    11161 gtggtccgga ccgattgttc ttctaacctc tctcgatagc gatatgaacc acatcctggc
    11221 actggaaatg ggcgcctgcg actatattct caaaacgacg ccccctgctg ttttgttagc
    11281 gcgtttacgc ttgcatttgc gtcagaatga gcaagccaca gtgaccaaag gtattcagga
    11341 aacgtctctg actccctaca aagccctgca tttcggcacg ttgaccatcg atcccatcaa
    11401 ccgcgtagtc tccctggcta acactgaaat ctcgctctcg acagctgatt tcgaattatt
    11461 gtgggaatta gctacccatg ccgggcaaat catggaccgc gatgcattgc tgaaaaattt
    11521 acgcggcgtc agttatgacg ggctggatcg tagcgtggac gtggctattt cgcggttaag
    11581 aaaaaaactg ctcgataacg ccgcagaacc ttatcgtatt aaaaccgtgc gtaacaaagg
    11641 ctatcttttt gcgcctcatg cgtgggaata agcgatgaaa aaactgttta tccagtttta
    11701 cctgttattg tttgtctgct tccttgtgat gtctctgctg gtcgggctgg tgtacaaatt
    11761 taccgccgaa cgcgcaggca aacagtcgct ggatgatttg atgaacagtt cgctgtatct
    11821 gatgcgcagc gaattgcgtg agatcccccc acacgactgg ggtaagacgc tgaaagagat
    11881 ggatttaaat ctctctttcg atctgcgtgt cgagccgctg agtaaatatc atcttgatga
    11941 tatttccatg caccggctgc gtggcggcga aattgtcgct ctggacgatc agtacacttt
    12001 tttgcaacgt atcccacgca gccactacgt gctggcagtt ggtcctgttc cttatcttta
    12061 ttacctccat cagatgcgat tgctggatat cgccctgatc gcttttattg ccatttccct
    12121 cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa aactggaagc
    12181 agcggcgcaa cgatttggcg atgggcatct cagtgagcgt atccactttg atgaaggttc
    12241 gagctttgaa cgacttggcg tcgcatttaa ccagatggcg gacaatatca acgccttgat
    12301 tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac cgttagtgcg
    12361 cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc aggcgttgaa
    12421 tcgcgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg cccggctcga
    12481 tcgcccacaa aatgagcttc atcttagcga accagacctg ccgttgtggc tgtcaacgca
    12541 tctggcagat attcaggcag taacgcccga taaaacggta cggataaaaa cgctcgcgca
    12601 aggccattat gctgcgttgg atatgcgctt aatggagcgc gtgctggata atttgctcaa
    12661 taacgccctg cgctactgtc actcaacggt tgaaaccagc ctgctactgt cggggaatag
    12721 agcgacatta attgttgagg atgatggccc agggattgcc ccagaaaacc gcgaacatat
    12781 ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc tcaaccggcg gctgcgggct
    12841 ggggctggcc attgtccact ctatagcact ggcaatgggc ggtacggtta attgtgacac
    12901 cagcgaactg gggggtgccc gcttctcgtt cagctggccg ttatggcata acatcccgca
    12961 atttacctct gcctgacact atgcgcacgg tggtcaagtc accaccactg tgctataaaa
    13021 taaggatgtt gtaactaaag tgattaatat tatggcgcgt tacgatctcg tagaccgact
    13081 caacactact tttcgccaga tggaacaaga gctggctgca tttgccgctc atcttgagca
    13141 acacaagcta ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag aggatgagca
    13201 taatccgctt aatcgtattg aggtaaaaca acatctcggc aacgacgcgc agtcgctggc
    13261 gttgcgtcat ttccgccatt tatttattca acaacagtcc gaaaatcgca gcagcaaagc
    13321 cgccgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacttttcgc aagcagcgtt
    13381 ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata tcgtcacggt
    13441 tgaatcagaa ctcccctccg tggcacgttt tgaatgggta catcgtcatt ttccggggct
    13501 gattaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca ctttacgttt
    13561 tggctgggct aataaacata tcattaaaaa tttgcatcgt gatgaagtcc tggcacagct
    13621 ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg agtggcaaag
    13681 aaaactggag cgagaatatc aggatatcgc tgccctgcca cagaacgcga agttaaaaat
    13741 caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg tataagggag atcaaaaaca
    13801 agtacaacac gcctgcccta cgccgctgat tgcgctgatt aatcgggata atggcgcggg
    13861 cgtgccggac gttggtgagt tgttaaatta cgatgccgac aatgtgcagc accgttataa
    13921 accacaggcg cagccgcttc gtttaatcat tccacggctg cacctgtatg ttgcagatta
    13981 acccccggct ttcatactgc cgaccatctg ttctggccgt acccagcggt caaactcggc
    14041 ttcgctaaga taccccagcg caagggccgc cgcttttaag gtcagccctt ctttatgcgc
    14101 ctttttggcg atctcggcgg ctttgtcata accaatgtgg gtgttaagcg cagtcaccag
    14161 catcagcgat tcattgagta attgattgat tcgctcacga ttcggttcaa tgcccactgc
    14221 gcagtgtttg ttgaaacttt ccatgccatc tgccagcaag cgcaccgatt gcaggaaatt
    14281 atggatcacc atcggacgga agacgttcag ttcaaagtta ccggaagcgc cacccatgtt
    14341 gatcgccacg tcattcccca tcacctgaca gcagagcatg gttaatgctt cacactgtgt
    14401 tgggttcact ttccctggca tgattgagct gcccggctca ttttccggga ttgagatttc
    14461 accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcaa ttttcatcag
    14521 tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agggcatcac aggtcgccag
    14581 cgcttcaaat ttgttcggtg cggtaacaaa cggtgcacag gtaatgactg ccagttcatc
    14641 tgctacgcga cgcgcatatt ccggatgggt atttagtcca gtacccaccg ctgtaccgcc
    14701 cagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat tatgctccag
    14761 catcgctacc cagccggaaa tctcctgccc cagcgttaac ggcgtggcgt cctgcaagtg
    14821 ggtacgaccg attttgacga tatcagcaaa tgcgcgggat ttttcactca gcgtctgggt
    14881 cagggtttta agttgtggaa tgagttgctt gcgcagcgcc agtagtgccg caacgtgcat
    14941 cgccgtcgga aagacatcgt tggaactttg gcttttgttc acgtcgtcgt tagggtgaac
    15001 tttacgttcc atcccgcgca cgccaccgag taattcactg gcccggttag ccagcacttc
    15061 gttcatattc atgttacttt gcgtgccgga gccggtctgc cagatagcca gcgggaattc
    15121 gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc tcgctttctc
    15181 ttcagacaac aagcctaaat cttcattaac ttttgccgct gcgcgcttgg ttagcgccag
    15241 cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct ccagcgagcg
    15301 ctgagtttgt gcgccccaca gcttatctgc cgggacatca atcgctccca tcgaatcttt
    15361 ttcgctgcgt actgtattca tggcctgctc ctcacctgat taattttttc tttctgtttt
    15421 gctttcgtta agcaactttt agctcactca ttatttacca tttgataaca aacgtttggt
    15481 ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc acacaacggg
    15541 tgcattgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc acaaggataa
    15601 acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt tccggatatt
    15661 caacacattc caggctctta atacttccct gcgccaatac agctgccgga ccaccgatac
    15721 tgccgaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg ctgcggttgc
    15781 ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca taagaatcca
    15841 tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc gttttggccg
    15901 gacccgcgta gtaaatcgga tgatctttaa tatactgcgg cagcccttca ccgttatcca
    15961 tccgctcttt cagtttggcg tgagcaatat cacgaccgac gataatcgtg ccgttaagcg
    16021 ataagcgtgt agaaacggga tactgcgaca actgtgcgag gatctctttc atcggacggt
    16081 tgaggtcaac gcgcaccgct tcgccttctc ctgctttgcg cagctcttcc gggatatatt
    16141 tgcctggatt atgttccagt ttttcgatcc agatcccctg acggttgatc ttcgctttga
    16201 tattacggtc agcagaacag gagacgccca taccgaccgg gcaggatgcg ccgtgacgtg
    16261 gcaggcgaat cacgcggatg tcatgagcga agtatttacc accaaactgc gcacccagac
    16321 caagattttg cgcttcgatc agcaattctt tttccagttc cacatcgcgg aacgcctgac
    16381 cgtgctcatt cccttccgtt ggcagttcat catagtattt agcggaagcc agtttcaccg
    16441 ttttaagatt cgtttctgca gaagttccac caataacgaa cgcaatatga tacggaggac
    16501 aggccgccgt acccagcgtg cgcatcttct caaccaggta atttttcagt tttcccggcg
    16561 tcagtaacgc tttggtttcc tgatagagat acgtcttgtt tgccgaaccg ccacctttgg
    16621 cgatacagag gaatttgtac tcgtcgccat caacggcata aagatcgatc tgcgctggca
    16681 agttggtgcc ggtattcacc tctttataca tatccagcgg cgcgttttgc gagtagcgca
    16741 gattatcctc gatataagtg ttataaacac cgcgcgccag cgctgcttca tcaccgccac
    16801 cggtccatac acgctgccct tttttaccaa caataatcgc ggtgccggta tcctgacagg
    16861 ttggcagaac gccttttgcc gcgatgtcgg agttacgcag gaattgcagc gccacatatt
    16921 tatcattttc gctggcctcc gggtcacaca gaatgtcggc cacctgttgt tggtgagccg
    16981 gacgcagcat aaatgacgca tcatgaaacg cctgacgcgc caacagagtt aacgcttcgg
    17041 gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt tcgctggtta
    17101 gcaggtaata ctcagtatca tcttttttga gtggaaaagg agcctgataa tgaaagggtt
    17161 tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc gggtgttccg
    17221 ttgccctgtt aaaagcgagt aacaatatcc tacacacttt tttaacaaaa actgagacta
    17281 gtacgacttt ttgcggctcc aggttacttc ccgtaggatt cttgctttaa tagcgggatt
    17341 aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag tgcaaaacta
    17401 tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc cgtccagcca
    17461 gccgatggcc gagttgtgga tgggcgcgca tccaaagagc agttcacgtg tgcacgatgc
    17521 cgcaggagat atcgtttcac tgcgtgatgt gattgagagt gataaagcga ctcttttggg
    17581 agatgccgtt gccaaacgct ttggcgagtt gcctttcctg ttcaaagtat tatgcgcagc
    17641 ccagccgctc tccattcagg ttcatccaaa caaacgcaat tctgaaatcg gttttgccaa
    17701 agaaaatgct gcaggtattc cgatggatgc cgctgagcgt aactataaag atcctaatca
    17761 caagcctgag ctggttttcg ccctgacccc tttcctcgcg atgaacgcgt ttcgtgagtt
    17821 ttccgagatt gtctccctgc tccagccggt cgcaggtgca catccggcga ttgctcactt
    17881 tttacaacag cctgatgccg aacgtttaag cgaactgttc gccagcctgt tgaatatgca
    17941 gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gccctcgaca gccagcaggg
    18001 tgaaccatgg caaacgattc gtttaatttc tgaattttac ccggaagaca gcggcctgtt
    18061 ctccccgctg ttgctgaatg tggtgaaatt gaatcctggc gaagcgatgt tcctgttcgc
    18121 tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga actccgataa
    18181 cgtgctgcgt gcaggtctga cgcctaaata cattgatatt ccggaactgg ttgccaacgt
    18241 gaagttcgaa gccaaaccgg ctaacaagtt gttgacccag ccggtgaaac aaggtgcaga
    18301 actggacttc ccgattcctg tggatgattt tgccttctcg ttgcatgacc ttagtgataa
    18361 agaaaccacc atcagccagc agagtgccgc cattttgttc tgcgtcgaag gcgatgcaac
    18421 gctgtgtaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt ttattgccgc
    18481 caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt acaacaagct
    18541 gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc ttataacacc
    18601 ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat aaatcgctgg
    18661 tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca tggtatacag
    18721 gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg caactcaaac
    18781 tgacagctcc tgaatccaac ctggaagtca gttatcaaaa ctatcatcgc ggcgtattca
    18841 gcagccagtt gcaactgttg gtgaaaccca ttgccggaaa agtaaatccg tggattaaaa
    18901 gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg cttgcccagc
    18961 ttaaaaaact gaatctgatc ccgtcgatgg catcaattca aaccacgctg gttaataacg
    19021 aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag attaactccc
    19081 gcattggtta cagcggagat tccagttccg acatttcgct caagccactg aattacgagc
    19141 aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgca gacagagacg
    19201 gcaaagctat ctccctttcc ggggaggcgc aaagtggtcg gatagacgcg gttaacgaat
    19261 acaaccagaa agtgcagtta acctttaata atctgaaaac cgacggttcc agcacgctgg
    19321 caagttttgg tgagcgcgta ggaaaccaaa aactgtcgct ggaaaaaatg accatttcag
    19381 tggagggcaa agaactggca ctgctggaag gcatggagat cagcggtaaa tcggatctgg
    19441 tcaatgacgg taaaacgatc aatagccaac tggattactc gctaaacagc ctgaaggtac
    19501 agaatcagga tctgggcagt ggcaagctga ctttaaaagt cggccaaatt gatggcgaag
    19561 cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg gcacagccag
    19621 aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc tttagcgctc
    19681 tgccgctgat gctgaaaggc gatccggtga ttactatcgc gccgctaagc tggaaaaaca
    19741 gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca acgactaaag
    19801 aagcgccgca aacgctggcg caggaagtgg accgctcggt taaatctctg gatgcgaaac
    19861 tgaccattcc ggtggatatg gcaactgagt tgatgactca ggtagcgaag ctggaaggtt
    19921 atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca tcagcaatgg
    19981 ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc ctgcaatatg
    20041 ctaacggtca gataacgtta aacgggcaga aaatgccact ggaagatttc gtgggtatgt
    20101 ttgcgatgcc gacattaaac gttccggctg tacccgctat tccgcagcag taattcacag
    20161 ccaaaacgcc ggagattttt ctctccggcg ttttttctta cttcagcata aagtcatact
    20221 gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg ccatcgcgct
    20281 tttcccgtcc tttatcggca gcaagaaaga aattcatccc ttgtaacggc ccttcgctga
    20341 cccgatagtt cataccccct aagatacccg ctccttcaat ccgcacatct cgtttactgg
    20401 attcatagcc gcgggtcaca atcggtgcaa atgtcagcgt aaattgcgga gttaaacgat
    20461 agttgacgcc caattgccag gactgcattc cttcacggtt acgatcaaga cttaatgaaa
    20521 agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc aaataaccac
    20581 cactgccaaa tacgcttccc cacggcgcat tccaggtcaa ttgtccacta accaacgccg
    20641 tttcattggg ctggctggta ttacgactta aaccttcaag ttgcgcgtaa aagccgagca
    20701 actccccttt caacgtataa ttcgggtcaa gcggtatgct tttctcgaga atactttgta
    20761 tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg gtatcatcat
    20821 gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc gtctggctgt
    20881 tataaacacc ttcatcacga ataccggttg ttgaatcggt ccataaaaat actcgtgaac
    20941 gccacattcc ccacagatca atatttggcg ttaaagccca ataacctaat gctccttcag
    21001 aaacagtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga taaggtaagc
    21061 taccataact ataatccgtc ccgaagcgac ctaattttag caggctattt tcactgggtt
    21121 ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga taccaccggg
    21181 tactcatatc agcacgagca cctaatttat aaacataata cgccccgcct tcaacgccaa
    21241 taattattgg aataataacc actattgaaa tcgagcaatc ctccctgtac ccatgcgtaa
    21301 atatctcttc ccgtgccagc actgggctta tcagccctgc gtaattcgtt tttcagtctg
    21361 atgcgtagtc ccgcttcatc gtcagccagt tgtgccgcgt aagcagaggt aaggccagag
    21421 gcagccgtca ggcaaataac ggccatggcc actatttttc tcatagttgc acctttgatg
    21481 ttctgattat ttttattgaa tattaactag tgatatcgtt gattaattgc tgctgcactt
    21541 ttttacgatt atcaatttca acaacgattt ctttgaattt tttatccgtg agcggataaa
    21601 accagataat gacgaatgcc agtagcataa atccgcaagg tactaaggca attgatgtgc
    21661 ggatgcccgt aataacttcc ggcgtttgcg cctgattggc gatatatccg cttaatccca
    21721 aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta aatgagaata
    21781 gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat tctacggtat
    21841 cagcttccag tgcccacatc acggtcatgg taacgccctg gccaattgat gcgatggcca
    21901 acgcaaccaa cgccaccggc agtgaccaga cggaaaccca gaagaacagc agataaccgc
    21961 aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc gcgaccatcc
    22021 ccggcaccag cggtgccgat gccacagtac cgaccaggtt ttgcaccagt accagcacag
    22081 tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag gcgctgaccg
    22141 cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc gggcgattcc
    22201 gtttcagggt ttgcagactg atcttcaatg acggctgcgc cacgatacgt accacattct
    22261 cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc agcacaattg
    22321 tccagaaatg atataccgac accatctctt ccgggctgga attcttaatg ctcggtccta
    22381 tcagaaatgc caggcagaca aaggtcaatg aagcgcaatc ccgcgagccg cgcccagacg
    22441 ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag gaatattcac
    22501 caggctgtag caaagcccga gtcccatgta ggtcaaatat gcatacacaa ctttgctgct
    22561 atggctccag tcggtcggca cccagaatac cagcacgctg aagatcatta acggcgcagt
    22621 accgaagagt aaaaacgggc ggaattttcc ccatcgggta ttcacactgt ccaccactcg
    22681 tccggcaaag acatcggcga aggcatcgaa tacccgcacc agtaacagca tggtgcccgc
    22741 cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga gcgcccccat
    22801 tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg cgcgccagga
    22861 gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt catgccggtc
    22921 cagcgttttt gcagcagaaa agccgccgac ttcggtttgc ggtcgcgagt gaagatccct
    22981 ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg caaaatcggc gaaattccat
    23041 acctgctcac cgacgacagc gctgacgcga tcaaagacgc ggtgatacat atccagccat
    23101 gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcaggcc ggctaacgta
    23161 tccacaccgt attcggtgat gataatcggc tgatgcagtt tctcctgcca ggccagaagt
    23221 tctttttcca gtaccttctc tgccgtttcc aaatcgccgc tttggacata ccaaccgtaa
    23281 taacggttca ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg agcgtcgcag
    23341 aacattacat tgacacaggt gatcggacgc gtcgggtcga gtttacgcgt tgcttccgcc
    23401 agtggcgcaa aatattcccg tgcaccttgc ggacgggtat ccggttcgtt ggcaatactc
    23461 cacatcacca cgcttgggtg gtttttgtca cgcgcaatca gctctttaat cgcctgtaag
    23521 tgcgcctgct gggtttcccc gttgactgcc tcttcgctgt acagttcttt cggcttgttg
    23581 cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagctgcagt ttcatcaatc
    23641 accacgattc catgttcatc tgcccagtcg agcatctctt cagcgtaagg gtaatgcgag
    23701 gtacggtagg agttggcccc aatccagtcc attagtgcgt gatcgtgcac catcagcacg
    23761 ttatcgaatc ctttgccgcg caaatccgca tcttcatgac gaccaaagcc agtaaagtag
    23821 aacggtttgt ggttgatcag gaactgttcg cccttcactg ccactgaccg gataccgacg
    23881 cgcagcaggt agatatcaca ctctgtctgg cttttggctg tgacgcacag ttcatagaga
    23941 taaccttcac ccggttgcca gaggtgcgga ttcactactt gcaaagtccc gctggtgcct
    24001 tgtccagttg caaccacctg ttgatccgca tcacgcagtt caacgctgac atcaccattt
    24061 gccaccacct gccagtcaac agatgcgtgg ttacagtctt gcgcgacatg cgtcaccacg
    24121 gtgatatcgt ccacccaggt gttcggcgtg gtgtagagca ttacgctgcg atggatcccg
    24181 gcgtagttaa agaaatcatg aaagtaagac tgctttttct tgccgttttc gtcggtaatc
    24241 accattcccg gcgggatagt ctgccagttc agttcgttgt tcacacaaac agtgatacgt
    24301 acacttttcc cggcaataac atacggtgtg acatcggctt caaatggcgt atagccgccc
    24361 tgatgctcca tcacttcctg attatttacc cacactttgc cgtaatgagt gaccgcatcg
    24421 aaacgcagta caatacgctg gcccgcccaa cctttcggta taaaaacttc gcgctgatac
    24481 cagacgttgc ccacataatt acgaatatct gcatcggcga actgatcgtt aaaactgccc
    24541 ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc accaatgctg atcaattcca
    24601 cagttttcgc gatccagact gaatgcccac aggccgtcga gttttttgat ttcacgggtt
    24661 ggggtttcta caggacgtaa cataagggac tcctcattaa gataaaaata ctggtcaacc
    24721 tttaatctga ttagttaacc aattccgtgt tgcacacagt tatctgtgag aaacccatgg
    24781 aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa aaaccaaaaa ctgcaatacc
    24841 cacagcgtga tattgatcgc attaatcttt aaaacagcgt tgacagaagc cacaaaataa
    24901 gcaatcgaac gttcattcga acatattggg aacgataaac cctcatgata tattgctacg
    24961 atgaatattt aggtggaatt ctccgatccc tgggaggatg atttttgcac agagaaatta
    25021 cgatatgaaa cgcgggctca gttgtcacca gcccgcggaa agagagagag gatcaggatg
    25081 cggttaatat accgccaatc atcgcgcgta atccctgagc aaacgacgct tcacgcgcca
    25141 gatcttccgc ttcgatatcg gccagtacac cgtaggtcag atccagcagt aaacgtgaag
    25201 tcattgccgt gttgatgtct gggcttatct cgcctttttg ctgggcatca cgcatccgct
    25261 gggcaacaaa ttccgtgatc gtcatatgct tatttttaag catggcggcg acctgtgggt
    25321 tacgcatccc ttccgccata atttcgacca ccagcgctcg ttgcccaaag gcttcatggg
    25381 tgagagagac aatagactcg accatatagt caacgaaatg aatcccttca atcggttccc
    25441 ggaaacgggc cagcgccctc tcctggtcct gtaagataat cgcctgaatc aaggcttctt
    25501 tggagatgaa atggtgatag agcgtcccgg gactaatggc gcaggattta cagatggctt
    25561 tcatcgaggc actgtgaaat ccattttctg aaaaaatctc tctggcagca ttgaggatcc
    25621 gggtccgtgt cggttgtgct tcagtctgca tgttatccat cattaaacca ttatagttag
    25681 catgaataac tagtttaccg cgttgtcagg tgagcgccta tcattgctgt tgaatagtgc
    25741 gaaggcacac tcaattcgta agctttttat ccagatattg gttaaccaat agccgtgatt
    25801 tggctgccag ttgtttagca gagacgttga cgagttgcac acgctatccc ggtaaacaac
    25861 acatacggct gtttaccggt tcgttagtgt attaattgag ttcctgtacc ccatcaccgg
    25921 agacggtgag aatttgtccg cttacccagc tggctgcagg cgaacaaagg aacagcgccg
    25981 cgttagcaat atcttgcggt tggcccagac gtctgattgg tgtgtgttgc aacattttct
    26041 gttcaatttc tggggtaata acggatttca gggcattggt taatattgcc cccggcgcaa
    26101 tgccattcac ccgaatattt ttttcaccca ggtcaaacgc catatttctg accagatgac
    26161 tggccgcagc tttagatgat gcataggagg tcatgtttat atttttattt tctgccgcca
    26221 tagaagtaat ggtcagaata acgccaccgc catttttttc catttctggc gcaataagtt
    26281 gtgacagatg gaaaaaagaa aatacattca gttcataagc gcggcgaaaa tctgccattg
    26341 gcatatcaaa cggtttaggc ccaccgccac cggcgttgtt aaccaggata tcaactttac
    26401 ccaacttact gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcgc
    26461 agcggcaggc aaatgcctga ccacccagtt gttgaatttc atctacaaca tgattagctg
    26521 catcggcgtt aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga
    26581 tttctttacc aatacctgca cccgcacctg taatgatggc gcattttccg tcgagtctca
    26641 ggttgtcaga attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc
    26701 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
    26761 aaagagagga ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
    26821 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
    26881 ctggcgtgct ggcccagata acgggtatat catcaagtgt ggtaggcgtt gcatcggtaa
    26941 atgccgccag cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
    27001 ttcctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
    27061 cggcgctgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
    27121 ggctggaagt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
    27181 tcgcacaata gccccccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
    27241 aggcgatacg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
    27301 tgttgtccgg gcgaaccgta tcaacgtcat caagataact ggcacgggaa gcgaaaatca
    27361 ccgggatagc tttttcttct gctatccgtc gcaggtcatc gctgcttcct gcagccccgg
    27421 caattaccac accatcgaca ccctgattca gtaatagtga aaaccgctgt gccagctgct
    27481 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
    27541 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
    27601 tgacgccact ttgcccgccg cgcagcgccg acgcctgccg attgcgcaca aatcccagct
    27661 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
    27721 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
    27781 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
    27841 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcctcact
    27901 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
    27961 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
    28021 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
    28081 cggcattggt agttctctta gcagccatga tgtcatcacc ctgatcccgg tcctgggcaa
    28141 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
    28201 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
    28261 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
    28321 gaccaataaa ggcattctgc caaccatgga cgccgccgtt ctgaaagcca ataacatcca
    28381 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
    28441 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgctggcatt
    28501 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggtc ttgtcggcct
    28561 ggtgattcca ttagtctggc cgattttcgc catgggtatt agcggcttag gccatatgat
    28621 caacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
    28681 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
    28741 ggaagtctgc ggtcaaaccg tcagcggtgc actgaccatc ttccaggcgc aattgagttg
    28801 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gtaaaatgcc
    28861 tgcgtttctc ggcggtctgc caggtgcagc gttagctatg tatcactgtg cgcgcccgga
    28921 aaatcgccat aaaattaaag gtctgctgat ttctggcctg atcgcctgtg tcgttggcgg
    28981 cacgaccgaa cctctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca
    29041 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
    29101 taccgacggc aatatcatcg actttgtggt gttcggtatt ttgcatggtc tgtcaaccaa
    29161 gtggtacatg gtgccagtag tggcggcaat ctggtttgtc gtttactaca tcatcttccg
    29221 tttcgctatc acccgcttca atctcaaaac cccggggcgc gatagtgaag ttgccagttc
    29281 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
    29341 cgaagcctta ggcggtgccg acaatattgt cagtctcgat aactgcatta cgcgtctgcg
    29401 tttgtctgta aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
    29461 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
    29521 agtaaaagat gaaatggtcg gtctgatgcg tactgtccag gcataaggat aagatatgtt
    29581 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acgcagtggg attatgtcgc
    29641 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
    29701 tgccccctgc attatcgagg cgctgaatca gcgcctgata cacggcgtgt ttggctacag
    29761 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
    29821 caccgccatc gatcctcagt cggtggtgta tggcccttct gttatctata tggtttcaga
    29881 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacac ccgcctatga
    29941 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
    30001 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgtgctgg cgaaaccaga
    30061 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
    30121 tgagctggag atcatggctg acctgtgcga gcgtcatggt gtgcgggtta tttccgatga
    30181 aatccatatg gatatggttt ggggcgagca gccgcatatt ccctggagta atgtggcgcg
    30241 cggagactgg gcgttgctaa cgtcgggatc aaaaagtttc aatattcccg ccctgaccgg
    30301 tgcttacggg attatagaaa atagcagtag ccgcgatgcc tatttatcgg cactgaaagg
    30361 acgtgatggg ctttcttccc cttcggtact ggcgttaact gctcatatcg ccgcctatca
    30421 gcaaggcgcg ccatggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
    30481 cgcagataaa atgaacgctg cgtttcctga actcaactgg cagatcccgc aatccactta
    30541 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
    30601 tatcgagcaa gaaaagatcg cgatcatgcc gggatatacc tacggtgaag aaggtcgtgg
    30661 ttttgtccga ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
    30721 aattaacgcc atccgcgctg ttggttaacc ccaattgcgc aacgtaaaaa atcgttgcgc
    30781 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
    30841 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
    30901 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
    30961 cacaatccct ggaaacactg actccccacg ttcaggtcat tgccaacgaa cccgatctgg
    31021 tgagctttct gactaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
    31081 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgaac
    31141 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
    31201 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
    31261 tcggtattat gagccggacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
    31321 tagcccaccg tgaccagatt accgcacttg atttagccgg tgatgaactt ggtttcccgg
    31381 gaagtctgtt cctttctcat ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
    31441 atgcaggcga agctgccgga ccggaaagca tctggcaggc gattcgtgaa ctgggggcgg
    31501 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
    31561 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtggcgg
    31621 atctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
    31681 ctgacgatcc cggcgtgcag ggagtggata tcattcacga atataccgtt gccgcgccag
    31741 ctgctgggtt atcccgcgag caaatccgcc aggcacagat taatggtctg gaaatggctt
    31801 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
    31861 ggatggtgct aattgcacca tccccttttc acgcaaggca caaagtcgcg cgatgtttgg
    31921 cggattcgat gcccagctca atcaactcca ttacctggat tgcctgactt gccggaaccg
    31981 gattttcacc atcgccattt aacgcatcac gaatagccgc atagtaagcc ggataattcc
    32041 ccggcactgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
    32101 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
    32161 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
    32221 gtgctgactc agcggctgcc agcatggtgc cgtgtaaaat cactcgccgc tgcggatagg
    32281 acaagatagc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
    32341 ccgtcatgct aaccggtaaa ccaaatagcg taatggcctg atcaagaagg tgcggtgcta
    32401 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgtacctgcg
    32461 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc ccccagcacg ccttccgcga
    32521 gcaaaccttt tagggtcaga aaatcgctat cccagcgacg gttatggaat acagacaaca
    32581 cacgccccag ggtttttgcc agcgcatcca gctctcgtgc ttgtgacagt gtcacggtaa
    32641 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttagct aacggaaaat
    32701 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
    32761 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcgctgc
    32821 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
    32881 tgctcgcata accataccca atcaccccaa cacggatgtt gtcgctcatg tcattgcctc
    32941 tcattgaagt atgatggcta tttgacacta tcctttaccc tcgctcaaca gtttaataac
    33001 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aatagcgatt tttccaacga
    33061 caaaagctaa atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt
    33121 aacatttacc ccctgaagtt aatggatcaa tcaacagaga tgtgggctgt aatgaatcgt
    33181 cttattgaat taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg
    33241 agtcacattg acaactatca gccacctgaa cagagtacta cggtacaaca caagtaagct
    33301 ctgcacttgt tgagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta
    33361 aaccaggaat ttattatctt gttcgatgtt gttgatgatt gtcagggata gtaaagcgcg
    33421 ttactctttt cagagcagtt tttcatccct gaatatttgc cat
//
LOCUS       DAPQZD010000040        33200 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPQZD010000040 DAPQZD010000000
VERSION     DAPQZD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.040"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            404..1471
                     /locus_tag="RO454_001812"
     CDS             404..1471
                     /locus_tag="RO454_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDY0933655.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1468..4203
                     /gene="rbbA"
                     /locus_tag="RO454_001813"
     CDS             1468..4203
                     /gene="rbbA"
                     /locus_tag="RO454_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDY0933656.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4203..5327
                     /locus_tag="RO454_001814"
     CDS             4203..5327
                     /locus_tag="RO454_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0933657.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5436..6287)
                     /locus_tag="RO454_001815"
     CDS             complement(5436..6287)
                     /locus_tag="RO454_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HDY0933658.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(6319..6588)
                     /locus_tag="RO454_001816"
     CDS             complement(6319..6588)
                     /locus_tag="RO454_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDY0933659.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKCSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(6578..8086)
                     /locus_tag="RO454_001817"
     CDS             complement(6578..8086)
                     /locus_tag="RO454_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDY0933660.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKATALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(8079..9437)
                     /locus_tag="RO454_001818"
     CDS             complement(8079..9437)
                     /locus_tag="RO454_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDY0933661.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSSGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(9514..9795)
                     /locus_tag="RO454_001819"
     CDS             complement(9514..9795)
                     /locus_tag="RO454_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDY0933662.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(9792..10265)
                     /locus_tag="RO454_001820"
     CDS             complement(9792..10265)
                     /locus_tag="RO454_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDY0933663.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            complement(10290..11036)
                     /locus_tag="RO454_001821"
     CDS             complement(10290..11036)
                     /locus_tag="RO454_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDY0933664.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(11235..11636)
                     /gene="nikR"
                     /locus_tag="RO454_001822"
     CDS             complement(11235..11636)
                     /gene="nikR"
                     /locus_tag="RO454_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDY0933665.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(11642..12448)
                     /gene="nikE"
                     /locus_tag="RO454_001823"
     CDS             complement(11642..12448)
                     /gene="nikE"
                     /locus_tag="RO454_001823"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDY0933666.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARTSEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(12445..13209)
                     /gene="nikD"
                     /locus_tag="RO454_001824"
     CDS             complement(12445..13209)
                     /gene="nikD"
                     /locus_tag="RO454_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDY0933667.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13209..14042)
                     /gene="nikC"
                     /locus_tag="RO454_001825"
     CDS             complement(13209..14042)
                     /gene="nikC"
                     /locus_tag="RO454_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDY0933668.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14039..14983)
                     /gene="nikB"
                     /locus_tag="RO454_001826"
     CDS             complement(14039..14983)
                     /gene="nikB"
                     /locus_tag="RO454_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDY0933669.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(14983..16557)
                     /gene="nikA"
                     /locus_tag="RO454_001827"
     CDS             complement(14983..16557)
                     /gene="nikA"
                     /locus_tag="RO454_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDY0933670.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     SLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(16668..17255)
                     /gene="acpT"
                     /locus_tag="RO454_001828"
     CDS             complement(16668..17255)
                     /gene="acpT"
                     /locus_tag="RO454_001828"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDY0933671.1"
                     /translation="MYRIVLGKVSTLSAAPLPLGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17310..18359)
                     /locus_tag="RO454_001829"
     CDS             complement(17310..18359)
                     /locus_tag="RO454_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDY0933672.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            18458..19708
                     /locus_tag="RO454_001830"
     CDS             18458..19708
                     /locus_tag="RO454_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933673.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(19712..20269)
                     /gene="dcrB"
                     /locus_tag="RO454_001831"
     CDS             complement(19712..20269)
                     /gene="dcrB"
                     /locus_tag="RO454_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDY0933674.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(20342..21007)
                     /locus_tag="RO454_001832"
     CDS             complement(20342..21007)
                     /locus_tag="RO454_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDY0933675.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21217..21462
                     /gene="tusA"
                     /locus_tag="RO454_001833"
     CDS             21217..21462
                     /gene="tusA"
                     /locus_tag="RO454_001833"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDY0933676.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(21564..23762)
                     /gene="zntA"
                     /locus_tag="RO454_001834"
     CDS             complement(21564..23762)
                     /gene="zntA"
                     /locus_tag="RO454_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDY0933677.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAATGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLSNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(23836..24462)
                     /locus_tag="RO454_001835"
     CDS             complement(23836..24462)
                     /locus_tag="RO454_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDY0933678.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            24603..24962
                     /locus_tag="RO454_001836"
     CDS             24603..24962
                     /locus_tag="RO454_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDY0933679.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(24965..25234)
                     /locus_tag="RO454_001837"
     CDS             complement(24965..25234)
                     /locus_tag="RO454_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDY0933680.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(25224..25820)
                     /gene="rsmD"
                     /locus_tag="RO454_001838"
     CDS             complement(25224..25820)
                     /gene="rsmD"
                     /locus_tag="RO454_001838"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDY0933681.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            25970..27466
                     /gene="ftsY"
                     /locus_tag="RO454_001839"
     CDS             25970..27466
                     /gene="ftsY"
                     /locus_tag="RO454_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDY0933682.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            27469..28137
                     /gene="ftsE"
                     /locus_tag="RO454_001840"
     CDS             27469..28137
                     /gene="ftsE"
                     /locus_tag="RO454_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDY0933683.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            28130..29188
                     /gene="ftsX"
                     /locus_tag="RO454_001841"
     CDS             28130..29188
                     /gene="ftsX"
                     /locus_tag="RO454_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDY0933684.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYASHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            29433..30287
                     /gene="rpoH"
                     /locus_tag="RO454_001842"
     CDS             29433..30287
                     /gene="rpoH"
                     /locus_tag="RO454_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDY0933685.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            30558..31661
                     /gene="livJ"
                     /locus_tag="RO454_001843"
     CDS             30558..31661
                     /gene="livJ"
                     /locus_tag="RO454_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDY0933686.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            31810..32076
                     /locus_tag="RO454_001844"
     CDS             31810..32076
                     /locus_tag="RO454_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDY0933687.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            32083..32610
                     /locus_tag="RO454_001845"
     CDS             32083..32610
                     /locus_tag="RO454_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0933688.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(32607..32990)
                     /gene="panM"
                     /locus_tag="RO454_001846"
     CDS             complement(32607..32990)
                     /gene="panM"
                     /locus_tag="RO454_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDY0933689.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
BASE COUNT         7936 a         8629 c         9023 g         7612 t
ORIGIN      
        1 gataataacg ctatgtgata gtcatcaggt ttccctgtta cggaaacctg tgcaacagaa
       61 tctgtgaacc tgtacagaat atttttggaa aaaacaccag cgaacggaag aacatcgcga
      121 tgtatcacaa gttgatggca atcaaataga caacatgaat ataactgtta gtatttaatt
      181 gatttcactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
      241 tactacccgt tttcactaag ccttccccat tggcgctcaa tgctctgtgc gctggtcgta
      301 tttgccattt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggtggcagtt
      361 gtaacacgtg tcgattgctt acagggatta tggcgggtcg attatggata agagtaagcg
      421 ccatctggcg tggtggggtg tcggggcact ggcggtggct gccgtcgtgg cgtggtggct
      481 gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac
      541 ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg
      601 ccagtttgtt cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca
      661 gcgactggaa gccatcgcgc aaatcaaaga agcacaaagt gccgttgccg ccgcgcaggc
      721 tttgctggag caacgccaaa gcgaaactcg tgccgcacag tcgctggtta atcaacgcca
      781 ggcagaactg gactccgtag caaaacgtca tacgcgttcc cgctcactgg cacaacgggg
      841 ggcaatttct gcgcaacagt tggatgacga ccgcgccgcc gctgagagcg cccgagctgc
      901 gctggaatcg gcaaaagctc aggtttctgc ttctaaagcc gctatagagg cggcgcgcac
      961 caatatcatt caggcgcaaa ctcgcgtcga agcggcacaa gccactgaac ggcgcattgc
     1021 cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt
     1081 tgccgagcca ggcgaagtgc tggcagcagg cggtcgggtg ctgaatatgg tcgatctcag
     1141 cgacgtctat atgaccttct ttctgccaac cgaacaggcg ggcacgctga aactgggcgg
     1201 tgaagcacgt ttgattcttg atgctgcacc agatctgcgt attcccgcga ccatcagttt
     1261 tgtcgccagc gtcgcccagt tcacgccaaa aactgtcgaa accagcgatg agcggctgaa
     1321 attgatgttc cgtgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     1381 tgtcaaaacc ggattgccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     1441 tgacgacctg gtggtgaggt tgccgcaatg atgcatctgg aactggttcc cgtcccgcct
     1501 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc
     1561 acactcgata ttccggcccg ttgcatggtc gggctgattg gaccggatgg tgtcggtaaa
     1621 tcgagcttgt tgtcgttgat ttccggtgct cgcgtcattg agcagggcgg cgtgatggtg
     1681 ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg
     1741 atgccgcagg ggctgggcaa aaatctctac cacaccttgt cggtgtatga aaacgtcgat
     1801 tttttcgccc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat taatgagctg
     1861 ctgaccagca ccggattagc accgtttcgc gatcgtccgg cagggaaact ctccggcggg
     1921 atgaagcaaa aacttgggct gtgctgtgcg ttaatccacg acccggaatt gttgattctt
     1981 gatgagccaa caacgggggt tgacccgctc tctcgcgccc agttctggga tctgatcgac
     2041 agtattcgcc agcggcagag caatatgagc gtgctggttg ccaccgccta catggaagag
     2101 gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc aaccggtagc
     2161 gccgaagagt tgcggcagca aacgcaaagc gccacgctgg aagaagcatt tataaatctg
     2221 ttaccgcaag cacaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa
     2281 aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     2341 gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct tggttcgaat
     2401 ggctgcggta aatccaccac catgaaaatg cttaccggat tgctgcccgc cagcgaaggt
     2461 gaggcgtggc tgttcgggca gccggttgat ccaaaagata tcgatacccg ccgtcgtgtg
     2521 ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaacctggag
     2581 ttacatgctc gtctgtttca tatcccggaa gcggaaattc ccgcgcgagt ggctgaaatg
     2641 agcgagcgtt ttaagctcaa cgacgtcgaa gatgtattgc cggagtcatt gccgctcggc
     2701 attcgccagc ggctatcgct ggcggtggcg gtgattcatc gcccggagat gctaatcctc
     2761 gatgagccga cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtg
     2821 gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat gaacgaagcg
     2881 gaacgttgtg accgtatctc actgatgcac gccggaaaag tgctcgccag cggtacaccg
     2941 caggagttgg ttgagaaacg cggagccgcc agtctcgaag aggcatttat cgcttatttg
     3001 caggaagcgg cagggcagag caacgaagcc gaagcgccgc ctgtggtaca tgacactacc
     3061 cacgcgccac gtcagggatt cagcctgcgc cgtctgttta gctacagccg ccgcgaagcg
     3121 ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac ggtgatcctg
     3181 atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctt
     3241 gaccgcgatc aaaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     3301 tttatcgaac agccaccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc
     3361 gatatcacgg tggcaattga gatccctccc aatttcgggc gcgatatcgc gcgtggtacg
     3421 cccgtggaac tcggtgtctg gatcgacggt gcgatgccga gccgcgccga aacggtaaaa
     3481 ggctacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg acagtccaca
     3541 cccgccagcc aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta
     3601 aaaagcctgc cggcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     3661 atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac
     3721 gtgaccccca ccacccgtag cgaattttta cttggcaaac agctgccata catcgcgctg
     3781 gggatgctga actttttcct gctctgcgcc ctgtcggtgt ttgtgtttgg cgtaccgcat
     3841 aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat cgccaccgga
     3901 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggtacggca
     3961 attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     4021 gaaggtccag gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     4081 cgcggaacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg
     4141 ctgatagcca taccgctggt gatgggctta agcatcctgc tgctgaaaaa acaggaggga
     4201 tgatgcgcca tttacgtaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg
     4261 gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag
     4321 cgaccgttac gccaggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat
     4381 cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga
     4441 tgatcaccgc cgatgagatg gatgccgggc tggacgccgg acgttatacc ttcgcgataa
     4501 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga
     4561 acgttgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta
     4621 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     4681 cgctggaaac acggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga
     4741 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc
     4801 gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga
     4861 tcatgatggc aaaggtctgg tcgatggggc tggtggtgct ggtggtgtcg ggattatcgc
     4921 tggtgctgat ggtgaaaggc gtgctgggcg taccgataga aggctcgatc ccgctgttta
     4981 tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga
     5041 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa
     5101 tgctttccgg tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     5161 tgaccatgcc gacgacacac tttgtcagcc tcgcgcaggc catcctctat cgtggtgccg
     5221 gattcgaaat cgtctggccg cagttcctga cgctgatagc gattggcggc gcgtttttta
     5281 ccattgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaaata cagtgcccga
     5341 tacgcaagct tatcgggcct acgcgatggt gcaatttatt gaattctagt ggttttgtag
     5401 gccggataag gcgtcacgcc gcatccggcg aggatttatt ttaccgaccg atgcccctca
     5461 ttcatcatca ggattttgct atacacatct tcctcgacgg cgttttttgc tgatgccatc
     5521 actcgtgcca gattcgcctc gttgtgatta ttcacatacg ctttgccgac tgcggtgata
     5581 aacgctttgc gcagatcgct ggcgatattc actttagcga cgcgataatt aacgaaactg
     5641 cgtaatatct ccggggcgat accggagccg ccgtggataa ccagcggcac cgggctgact
     5701 tcggcaatgc gcttcagcag ggggatatcg atccgcggta tatcgtccag gccatgaacg
     5761 ttaccaatag aaactgccag catgtcgcaa ccggtgcgtt cgacaaaggt cttcaccttt
     5821 tccggttccg ttttgcaatc ggcctcgctg acgtgatcat cttctttgcc gagaatcgcc
     5881 cccagctccg cttcaaccgg cacgccataa gatttacaga aatcgacagc ttcctgggtg
     5941 aaagcgatat tctcttcaaa agagaatgcg gcaccgtcaa tcatgaccga ggtaaagccc
     6001 gcgcgcaccg cctgtttaac atcctcctgg gttttcccgt gatccagatg aagactgacc
     6061 ggaacatcca ttttatcggc gtgacgtttc acgatttcat atatgtaatc gtatcccgac
     6121 agttgtgcat tggtgggcgc aatctgaatg aagttgggta atcccgaacg ctcaatagcg
     6181 tttaaaattg aaattgtcgt ctccagattg gtggtattaa acgcgcccgc taatacccgt
     6241 ttttcctgaa tgcggtcgag cagtatccgt ccattcacta atggcatatt gaaaacctca
     6301 ttttttaata tcagttaatt attcagcttc accgaagcgg tcgcgaaata aggcggtgag
     6361 agtctgtatg gcctcgtttt catcatcacc gtcgcagcgt atttccaccg tttttccgcc
     6421 ttttattccg gcgcgcataa tattcatcat actggtggca ttaatatcat taccctcata
     6481 gtgcagcgtc actttacagc tacatttttt ggcggctttc gccagacagg ccgccggacg
     6541 tgcatgcagg ccagtactgt ttctgacctc cacggtttta gtcaacatag tgctcctccc
     6601 gatgatgtaa tggcatcacg ccttcttgct gtaatttcaa ctgataccag cttttccatt
     6661 tgtgctgata acgctgtaat tgatgatatc ttgcccgatt cggtaacaaa cgttgggcta
     6721 tggtaacttg cggcggcgga atatccggtg ccgcggttaa taaggctccg acagaaaccg
     6781 cctcgctaaa tgcaattgct tcaaccggac gctgtaaaat atctgccttt aattgcagcc
     6841 aaagggggtt atgggtcgcc ggaccgatca cttttaacac gctggtgcca ggcacattaa
     6901 aacaggcaaa gcagttggca aattccatgg ttaatcccag tgagacgctg gaaattaaca
     6961 tgtcgatatt taacgtatcg ccaagcccat aaaataatcc gcgggtgtgg cgatttttat
     7021 agggtgaacc cgaaccgcga agatgcggaa taaagacggg aatgtcatca agcgaccagt
     7081 tccccgccag gtaatccgca tacccacgat tgagcgacgc ggcgatctcc tcatcagtta
     7141 gccggaacgt gttgcgcagc cactccagcg cgaaaccgcc cacgggcaga gaagcaaaca
     7201 gggtgaactg gttggcaagc gagtaacagc cgtttgccag cttgtcacgt ttggcctgtt
     7261 catccagcgt cggttgtgta tccagttgca gaatgccttc cgtggtgccc gtcgagttaa
     7321 ggatatcgcc cggcatcatc tgcaacgccc gcgcacccac catatggtca tgtccggcca
     7381 gcgtcacgct gaccggttgc gagaacccaa gcgttttgca gagcgactcg ctgacccatc
     7441 cggcgtgttc gcctggctgc accaatggcg caaatgctga acagggaacg tgtaacaacg
     7501 ccgtcgcttt cgctgaccat tcgcctttgc gcacgtccag acacagtgta cggctggcta
     7561 aggtgatatc cgttcgctgt tcgccagtca gccgccagag cagcacttcc ggtgcatgta
     7621 gccagcgcag gccgcgacga ttgcgcagcg gataatgctc cagcaaccaa cgcattttga
     7681 aggcagagta attgctgtgc agtggtaatc cgcaaatgtc ataaagtgcc gcactgtctg
     7741 cctcgctaag cgttgccaga tactcttcac cgcgacggtc ataccatgcc agcattggcg
     7801 tcaggatctc gccatgttcg tcaagaaaca cccctgactc gccaaaactg gcaatgctga
     7861 tccgcctgac aggcagcggt gaagcggcgt tcagttgcgc tatcgcctgg cggacctcct
     7921 gccagagggc gtcgatatcg aaatcgacat cgccctgtgg ggagatctgt tttgccgtca
     7981 cgaatttatg cgcgcccaac gtcgttgcgt ccaggcagga aaagcaggtg actttgcaat
     8041 tggtggtgcc aatatcgata acgatagcgg cgctgttatc aggcataaac cgtgctccgc
     8101 gtcattcgga aaatgccata cagcaccagg gcaataaccg gcaccagagc aatggcgatg
     8161 agattgccgt tgaagatttc cagcagccag aagcggaacg ggttaccgcc atcaaggaaa
     8221 ctggaaacct ggccgctgga gccttcggca aatttgaagt taacgtcttt cgccattccg
     8281 gtaataaacg gcgcgatttt ggtagcaatc cacaggtcgg caataatcac cgggatcgcc
     8341 gccagaacgg tgcggaaaat gttgccacgg ctggctaaag caatcatcga cgccattacc
     8401 gccaggttgg caaggtcgcc aagcggcagg acttcattac ctgggaggac aaacgcgata
     8461 aagaccgaga ttggcgttag cagcagggcg gtggagataa tcgccggatt acctacggca
     8521 acggcgatat ccaggccgat atagagatcg tcacggtcag ggtatttggc attgagatac
     8581 tttttaatgg cttcagacag cggtaataaa ccttccatca gaatgcgaac catgcgtggc
     8641 aggatgaaca tcacaccgcc aatgctgatg ccaagcagca atactttttt gaaatcgtat
     8701 ccggcaatta cgccgagaaa aatgcccaga atagtgccaa ccatcatcgg ttcgccgaaa
     8761 atgccaaact ttttctgtac ggtttccgga tcgatatgaa tacgattgag gccggggata
     8821 tggtcgatga ttttgtcgac caacagaccg accggaaaga acaccaccga agagagcgtc
     8881 ggcaaagaga tcccttccag gccaaagtat ttttgtacca gtggcgcaga ccagtcggcg
     8941 agtttaagta ctaccaccgc agcaatcgcc gccgccagca aaccaaagaa caggctgccg
     9001 gagctgtaat aaaccagcgt accggcaagg gcaaaatgcc agtagttcca catatctacg
     9061 tcaacggtgc gggttttatt taacgccagc atcgccacgt taatcagaat agtcagggga
     9121 ataacaaacg gggcaattgg cgaaccccat gtaatagcgg aaagcggcgg ccagcctaaa
     9181 tccaccaccg gtaaattaat tccggtgcgt tcaaccatcg cctgggccgc tggcccgaca
     9241 ttgctggtaa gaatatcgaa aatggcataa ataccgacaa aaccaatgcc gaccgttaat
     9301 gacgaacgta aggctttagc gggttttact ttgaaaaaga gagcaagaat aaataacacc
     9361 aacggcaaca ttaccgtggg accaaatccc aatatatatt gcacaatatt atagagggta
     9421 tgcgcgatat cgttcataat aaacctcttt ttcaggcgag agcgcacctc gcgttagcgc
     9481 ctgatgtagt gtgttgaaat taattattat taattacttt tttaggatag aaacaatttg
     9541 cgccaatact ttctcttcgc caataccagt aataattggc aagccgttaa ccaccggcac
     9601 gtcgagttca taaggcactt tagtcgttga aacaaccagt gctacgccgt cggtatttcc
     9661 aggtaaagag gcaacgttgg tttgagaata attaacgtta agaccgtgct ctttacaata
     9721 ttccatgact ttttctgtta ctgccgtgga tgtggcaata cccgtggcgc agacaaataa
     9781 aatggtttga ctcatatttc acctcgttct ttcattttgt ttaagattgc tgtgtgtatt
     9841 tctgttgttt ccatcgtaag cagggcgcac ataaagggcg tatcctgaat catctccata
     9901 atccacccca gggcatttaa ttgtttgtta ctttcgccta aggcgagcaa aaaaatcacc
     9961 ctgacaggta cgggatcagg gtcgccgccc atatcggcaa acactaccgg ttccggcagg
    10021 atgccgacgg caattgcatt gtgcctgaca tgtttatggt ccgtgtgcgg aatggcgaca
    10081 cacggtggtt cggtaggcag tcccgttggg tacgtcgcct cacgttccag tacatgatcg
    10141 gcaaaatcag ggaccaccag ttcggcggta accaacggtt gcgctaactg acggatgatc
    10201 tgttcaacgc ttaaccctgc cggaagtcgt tgaataaaat tttcctgaaa ctgtatgcct
    10261 tgcattttat tcctcttaaa aaagtgtact taactcactt cccgccgttg cagcacggtg
    10321 ccaagatagt atttatttcc ttttaaccag acgttgccaa actcaacggg taatccgcgg
    10381 gagaaaaaga ctaactgctc cagatgcaaa acgggtgcat cttcactgat atctaaaaaa
    10441 tgaccgcgtt cattaccaat taatcgcgcg gcatagcgac tttccgagta acgaattttt
    10501 ctttgcgaca aactttctat tgttggaaat aaattgtgtt gattaaaatc gatttccacg
    10561 atgccggggc atagctcaat attgatacgg ttctctatca gcatcgcttt ttcatcacca
    10621 attgaacgta aacgctcaag gtaaagaata tcctgaccgg gcgttattct taatttctct
    10681 gccacataac gattagccgg ttcaatccgt gacgtaataa cttcagtggt aaaatgtatt
    10741 ttctggcttt ccagcgattc cgcgaatgac aataatcctt cacctaacgg atatgccacg
    10801 ttctcttttt tgacaaatgt tcccttcccc tgaacctgaa tcaacacgcc ttcttttacc
    10861 agttgggcga ccgctttttt aacggttccc cgactcactt ccagcatatc cataatctgg
    10921 tattccgaag gaatgcgatc gtcttccacc agatccccgg tataaatact ttctcttatc
    10981 cactccacca cttgttgata gagcggtatt tgtgacgact tactcaatga tttcatggcg
    11041 cgcacctgaa catttacagt tgtctatgga caatcatgga caacttcaag cgtatagtag
    11101 gtgaacaggc gaaaaagagg atcgaagcgt ctcacatttt aggggaccaa atcgtgatca
    11161 aaaaaatgtg acgtgagtcg atatttggca ggagtctact gcgccgtcag gaaataacgg
    11221 cgcgttggga ggtgttaatc ttccttcggc aggcactgca aatgcccgtg ccgcacgccg
    11281 cgctgggcga tgacgtcgtc ggcaaaatgt tgtacgtcac ccatgtcacc tttcaacacg
    11341 gcgatttcca gacagtcgtc gtgattaatg tgcacatgca gagtagcaac cgagagatcg
    11401 tggtgatgat gctgggtgga gacaatacgg ctggctaagt cacgtttttc gtgttcatac
    11461 acatacgaca gcaccgcgaa accttgtgtg ccgtgttgtt gagtggcttc ctgcgccaga
    11521 gcgctacgca gaatatcgcg gatcgcctcg gaacggttgt tataaccacg acgctggctc
    11581 aggctgtcca gcgtctccag taaatcgtca tcaagcgtga tggtgactcg ttgcatttgc
    11641 gttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta
    11701 gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga
    11761 tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt
    11821 gggtgatgaa caggcaggcg gtgccaaact gttgttgcag ctttttcagc aggcgaatga
    11881 cgcccgcctg taacacgaga tcaaggttcg aaacggcttc atccagaatc agcagtttcg
    11941 gttcgaccgc cagcgcgcgt gccagacaga cacgctggag ctggccgccg cttaactgcg
    12001 gtgggcgttt gtcgagcacg ctgtcgtcga gatcgaccgc cttcagcatt tcgctggtgc
    12061 gcgccagttg ttcggctttt ttcagcgata gcaggtggcg catcggttca cgcaggatct
    12121 cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc ctgaaacacc atttgaatat
    12181 cacggcggaa cgctttacgt tgcgcgcgat tgagcttcgc caacggttcg ccgcgccagc
    12241 taatatttcc ctgtgagggc gactctaacc ctaccagcag ccgcgcgagg gtactttttc
    12301 cgcagccgct gcgtcccagc agggcgacgg tttcaccgct tttcagggcc agggaaacgt
    12361 tattcagcac cgcctggtgc tgatgttttc cgctaaatcc accgtgcgca tagtgatggg
    12421 aaaggtcgga gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg
    12481 cgcggaaacc aggctgcgcg ttaccgcatg tttgggggcg ttaaacagcg tttctacatc
    12541 gccctgttcg acaattttac cgtgagacat taccgccaca tcgtctgcca gacgcgctac
    12601 tacgcccata tcatgggtaa ccagcagcat tcccggtgct tgtttttgca taatgctttc
    12661 cagtagatcg aggatgcgtg cttgtgctac cacgtcgaga tcggtggttg gttcatcggc
    12721 gatgataaac ggtgattcgc acagcaccgc catcgcaatc atcatgcgct gcaacatgcc
    12781 gccgctcatc tcgaacgggt agagcttcag cacgcgcgcg gcgttttcca gccccaccgc
    12841 ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc
    12901 acgcgcgtgg gtgtgcatgg tgtgcagcgg attaaaggca ctgcgcgggt tctgcatgat
    12961 ggtggcaatt ttgatgccgc gcagggcgca gggagaaacc ggttttccat cggccaaaat
    13021 ttcccccgcc gtctggcgaa cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag
    13081 cgacttcccg ctgccactac cgccgactaa cgccagcaca cgcccacgtt gcagggttaa
    13141 cgatacgccg tgcaccagcg gctgcgcggc ctgtagcgcg atattacgga gttcaatctg
    13201 ttgtggcatt agtgtgcgtg ctccgtcacc agatgagggt ccagatgatc gcgcagtgcg
    13261 tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt
    13321 tgcagcggct gggtccagat atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg
    13381 gtcggcgcgg tcacgccgag accgaggaag gacatccccg cgacgtgtag catcatatga
    13441 ccgatatcca gcgtcgccag caccagcagc gaggggatca ccgcacctgc cagatgatca
    13501 ataaacaccc gcacatggcc cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg
    13561 cgcagtgaaa tcaccagact gcgcaccatg cgcgcgtacc atgcccagtg cgaaagggcg
    13621 atagcgataa ttacgttggt caacccggtg ccaagcacgc cgaccataaa gaacgacaga
    13681 atcgaggttg ggaaggtcat aaacatatcc gcgacgcgca tggtggcctg atcaacgcgc
    13741 ccgccaatca gcccggcgct gccgccaata atcagcccta atgtcagcac caacagcagg
    13801 caggccatta ccgagccgag cgacacgcgg gtcgctgcca tcagccgcga gaaaatatcg
    13861 cgccctaaat gatcggtgcc cagccagtgc tgcgcatccg gcgagagcag gcgcgacggc
    13921 aaatcaatag cctgagggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc
    13981 agcagggcga taatgatcag cgccaggcga accgaccagc gggaggagag gaaaaagttc
    14041 acgcgtgcgc tccttcatga cgacgaatac gcgggtccag cgcggcgttg agcaaatcga
    14101 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
    14161 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
    14221 tgttttcgat aatcatcgtc ccgccaatca gttcgccaat gtgcatcccc acggcagtga
    14281 tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg tcattcaggc
    14341 cgcgcagccg cgcccaggtc acatgacgct gaccggcgac gtccagcata ctggcgcgca
    14401 gcaaacgcgc gttaatcgcc agcgacataa aggcgatgga taccgcaggc aaaatgatgt
    14461 gctgccagcc gccgtaaccc atcgcgggta gccattgcag atacaccgaa aacgccatca
    14521 ccagtaaaaa cgccagccag aagttaggca tcgacacgcc gagaaacgcg atgaaacgga
    14581 cggcgaaatc gggcaagcga tcgcggtggc gcgccgccca gataccgagc ggcacggagg
    14641 tgagcagaat taataccagc gccgcacccg ccagttccag cgtggcgggc aggaagttca
    14701 gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
    14761 ccttccatag ccaggtgcca tactggacgt acagcggctg atccagcccc agcatagtgc
    14821 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaaa taatcaagcg
    14881 ccgggtcgcc ggtgccgagg cgcagcatca aaaaaatgat caccgaggcg gcgagcacca
    14941 tcgggatcag cagcagaaag cggcgtaata cataacgcaa cattaaggtt tcaccggttt
    15001 aatctgttcg aacgggattt cggtggcgat cggcgcgtag gggatgttac ccagttccgg
    15061 ttttgaaacc accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag
    15121 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
    15181 ttcgccgatc tctttatcaa tcagcgactt gtcggctaaa ccttgctgtg cctggaagtc
    15241 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
    15301 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
    15361 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
    15421 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
    15481 accatttttc tcgcggatgt ctttgccagc aggcagcgtc caaccggctt tttccagcaa
    15541 cgctttcgct ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggcac
    15601 agaaggggca aacagggtgt cggcgacctg ctgggtacca tacaacgcgt tatcaatcag
    15661 cgattttttg ttcaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
    15721 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta
    15781 agccgggttc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
    15841 cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt ccgggatcac
    15901 gttaaaggtg atctttttaa tcgccggttt ttcgccccag tagttttcgt tacggacgaa
    15961 gacatcgtac tgattcagtt tcgattcctg caaaacccac ggtccggtgc caatcggcgc
    16021 ttttatcccg ttcatggttt cgtgattttt aaattgtgac ggggcaataa agcggaaagg
    16081 acgcggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag
    16141 ctcgtttttg ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg
    16201 acgttgacgg ttatcgagca ctgcacggaa gttttctgcc gccgcctctg catcaaacgg
    16261 ttcgccgttg gagaatttca cgtcatcacg caaggtgaag gtccaggttt tgccatcttc
    16321 cgaatgggtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
    16381 cagcggttca taaaccatgc tctgggcgaa catttggtta ggcgtgtaaa ggtgcgggtt
    16441 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
    16501 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtag agaacatggt
    16561 aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat
    16621 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
    16681 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg taggggtgca gatcgccagg
    16741 cttaaatttt ctagctgaca atggctgact gacagcgagg agttataggt gctgtctacg
    16801 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
    16861 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc
    16921 ccgaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
    16981 atatcgcagc cgacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg
    17041 cttaagttga accatagcgg cgtttccggc gcgaatgcag gtttgccttg ttcgccatag
    17101 atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt
    17161 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaaccca gtggcagagg agctgcgctt
    17221 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
    17281 tgaaagtgcg tatcgtaaca cttgtcgcct catcccggta accgactttt cggtctgcct
    17341 ggccccagta aaatcgccag tttgctgccg cctttggtgg tttccatcca gattttacac
    17401 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccaaatt
    17461 aacaatgaaa gaaataccac catggtggac atccccaggc gatggcccat catccgtggt
    17521 tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac cagaatacat
    17581 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgca
    17641 ccgatattgg gcacgtagtt gagcaaaaac gccagtactg cccacatcag cgcaaactgc
    17701 acccccatca gctccaaccc cagccagacg attacgcctg tccataaact gagtagcgtc
    17761 tttaacgcca gataatgcga aacgccttta agtgcgcggt gtagtccggc gatgtgaatc
    17821 tgtggattat tcaacgcgaa acgcattttg taagggacgt ggcgcacttc aaacagcata
    17881 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
    17941 agcgccgtgg tgaaggtaac cactttttcc gagtccatcc gctgtagtat tcgctccggc
    18001 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc
    18061 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
    18121 accagcgcgg tgagtgcgat cagcatcacc acaaccacaa tcgtaatggc aacggggcgt
    18181 tgtactcccc gacgaataaa ccaggtgacg agcgggttga gaacgatggc aaaaaacagc
    18241 gccagtaata gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
    18301 agcgaggcca acttgagcag aatgcgcatg cccgttttat cgggttgagg ggtttccatt
    18361 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
    18421 ttcttactgc tgattttcac gtcagcacat ggtaaaaatg aaacactgtt gtaaaaatgt
    18481 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
    18541 gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
    18601 cgctgtatta ccaggctatg tccatgatgt gatgggcttt agtgccttct gggcagggtt
    18661 ggttatcagc ctgcaatatt tcgccacctt gctgagccgt cctcatgccg gacgttacgc
    18721 cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttatgagcgg
    18781 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
    18841 gtgcctgggg cgcgtaatcc ttgggattgg acaaagtttt gccggaacgg gatcgaccct
    18901 gtggggtgtt ggcgtggttg gttcgctaca tatcgggcgg gtgatttcgt ggaacggtat
    18961 tgtcacttac ggggcgatgg cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg
    19021 cggattgcag gcgttagcgt taatcattat gggcgtggca ctggtggcca ttttgttggc
    19081 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gtgcggtgct
    19141 tgggcgcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgttat
    19201 cgccaccttt atcacgttgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
    19261 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
    19321 tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt gagataatcg gcctgctact
    19381 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggcggg
    19441 gttttcgctg gtgttccctg cattgggcgt agtggcggta aaagcggttc cgcagcaaaa
    19501 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgaccgg
    19561 accactggct gggctggtga tgagttgggc gggcgttccg gtaatttatc tggcggcggc
    19621 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ccccggtgga
    19681 aatacctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
    19741 tttctgcggt ggtctgtgct ttttgctgat tgtcagcggg cagcgtaatt tgcatggtta
    19801 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
    19861 agataatgct gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
    19921 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccagcaccg
    19981 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttctgc ccggtggcgt
    20041 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
    20101 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtcgcgt
    20161 tactttctgc gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
    20221 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
    20281 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
    20341 tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc aacagcatat
    20401 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
    20461 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
    20521 aggcaatgaa gaagaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg
    20581 gtgccagcca ccagtgacga ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct
    20641 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
    20701 gggcgagcgc gccgaatcct tgccatgacc ccatataaaa cagcgatgag atgacgtagg
    20761 agatcagcaa tgcggggatc attaccgcaa agatgatgcg acgagccagc ggcgcgccaa
    20821 aaatacgcac ggtcaggtca gtagcgagaa agataaacgg aaagctaaac gcgccccagg
    20881 tggtatgaaa accaaaaatg gagaccggaa gctgaaccag atagttactg gaggtgatca
    20941 ccagaagatg aaataacgat aaccagaaca acgccttata gcgttgcgtt tgcgagaaaa
    21001 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtgcgacac
    21061 gctattttat acgtgctgac ccttttttcg agccgccgca tgatactgct ttacgttgac
    21121 aatgcaatgg ttaattttca cgcaatcgtt aacctggttt gcttacggac tcacagggcg
    21181 taaactagcg ccgttttttt atgtgatgag aagaaaatga ccgatctctt ttccagccct
    21241 gaccacacac tcgacgcact tggcctgcgc tgcccggaac cagtaatgat ggtgcgcaaa
    21301 acagtgcgca atatgcagcc aggtgaaacg ttgctgatta ttgctgacga tccggcgacg
    21361 acccgcgata ttcctgggtt ttgtaccttt atggaacacg aactggttgc taaagagacg
    21421 gatggactgc cttatcgtta tttgattcgt aaaggcggtt gatgggggaa gattggcttc
    21481 gatgccgcct tttcccctca ccctaaccct ctccccagag gagcgagggg accgatcgcg
    21541 ctcaatgttg cgatcggttt gccttatttc ctgcgcaaca gccttaacgc attcgccgtt
    21601 accagcaccg tcgcaccggt atctgccagc actgccagcc acagtccggt catccctaac
    21661 agcgtcgtga cgaggaaaac gcctttcagc cccagcgcaa tagtgatgtt ctggcggata
    21721 ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca gaccgcgcag gtggttatgg
    21781 gttaatgctg catcggcggt ttccagcgcc acgtctgtgc cgctacccat tgcaatcccg
    21841 atggcggcag ctttcatcgc tggcgcgtcg ttaataccat caccgaccat cgccagcggc
    21901 gcatgttgat tcagctcggt caccgctttg actttatctt ccggcaagag gcccgcttta
    21961 aattccagcc ccagttcccc ggcgattgcc gctgctgcgc gtggattatc gcctgtaagg
    22021 atcacccctt tgacgcccag cgcgttcagt tcactgatag cagttgcagc atcggcgcgc
    22081 agggtatcct gcaacgcaat gacacccagc acgtcatcgt tacgtactac cagcactacc
    22141 gtttgcccgg cgctttccag ttcattaatc aaaccagcaa atgcatcagc aggatgtttt
    22201 ccggcagcgc atatcaacac gcgctcaccg ttaacctgcg cttcaatgcc agacccgacc
    22261 agcgcccgct gtgattgggc ggtaggaatg gcgagttcag caacctgtgc ttcgcgcaca
    22321 atggcctgtg ccagtggatg cgtcgcgcct tgctcgaccg ccgccgccag tgtcagcagt
    22381 tcagattcac taatacccgt tgccggatga atcgcggtaa cgcgcggttt accgacggtc
    22441 agcgtaccgg ttttatcaaa cgccacctgg gtaacacgac ccagctgctc cagcgccgcg
    22501 ccgcctttaa tcaacgcccc acgacgcgct gctgccgcca gcccggaggt aatcgccgcg
    22561 ggcgtggaga taactaaagc acacgggcaa ccaatcagca gcagcgtcag ccctttataa
    22621 atccactcct gccagctggc ggcaaacagc agcggcggca ccagcgttac cagcagggcg
    22681 accgccataa tcgctggcgt gtaaatacgg ctgaaacggt cgataaaccg ctcaataggt
    22741 gcgcggcgct cttcggcttc ttcaatcagt ttcagaatcc ggtcaatggc gctggcaccc
    22801 ggttctgaca gcacttccag cgttaccaga cggtctacgc tggtggcgcc tgcaggaact
    22861 ttatcgcccg tcgcgcgctc caccggaatg gattcgccgg tcagggcact ttcatcaaaa
    22921 ctggcaaacg gtgagagcag tttaccgtcg gcaggcaaac gcccacctgc cgcaacttca
    22981 atcacatcgc caggccgcag gctgtttatt gccacctctt cacgctcacc gttactcaga
    23041 cgcgtggcgg tttctggctt cagcgccatt aacgcgctga ccccttgacg cgcgcgactg
    23101 gcggcccagc cttccagtcg ttcaccaatc aaaaacagca gcaacaccat cgcagcttca
    23161 gccgttgcgc caataaacag cgccccagta gcggctacgc tcattaaggt ttcaatggcg
    23221 aagtagctgc cagatttgat cagccgtaat gcctgacgag caattgggta cagcccaacc
    23281 agcgtggtcg cgataaacgc cagttgacca aacggatgat tgaactgctc cagcccccaa
    23341 ctgattgcca tcatcacgat aagcgtaatc agcggcagat tctctttcag gcgtgattct
    23401 tgtggttcgt cggaggcctg ttcatcgcgc agggaatagc ccgctttttg caccgcagat
    23461 tcaacttgtg cccggatgtc attgtcggca tccaccacca gtttttcggt ggcgaacaac
    23521 acctgtacct gattcacgcc tgcaagctgg cgcacggcat tttccacttt gcgcgcacag
    23581 gcggcgcagt ccataccgct gactttccag ctatagcggg tgccggagac gttttcagag
    23641 agagttggcg tgctggaaca tgcgccgtcg cagcaacagt cgttggtgtt ctgtaccgta
    23701 gttagcggtt tgaacgcagc aaattgaggg gctttcttgc cgtgattgtc aggagtcgac
    23761 atggcatcct ccggttaagt tttttctcat taaccgaagg atacactctg gagtcgactc
    23821 cagagtcaag ttttatcaga gatacagcga gcggacgatg aggaagtgac cagcaaagta
    23881 gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg cgatagtgac tacccagcca
    23941 gacaaagtta ctgataaaca gcaacgaagc gccaacaaac gcagagagcg ccggagcggt
    24001 cggacggaag aaccacagtt cacctgccag ccacaccatc accagcgtca tgccgataaa
    24061 cgtgcagata ggccaacggt actcttccag gcgcgtccag ataatcgcca gtaacagcgc
    24121 gcccagaacc agcagcacca gtggcagagg ccagaagaaa gagagcgtca tctgactggc
    24181 gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt acatcagacg
    24241 ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag gcacacagcc ccgccagcac
    24301 cagatagcta atggcgtcga acatcggcgc ttgccaggcc agcaacagca ggagaagaag
    24361 ggttaacggt ttaaacaccc agcgttgcca ggttggccca cgatacgatg catccacaga
    24421 tagccatgcg gaaagacaga cagcgataaa cgaccaaagc atcttagttc cttgaattcg
    24481 ttattttttc gtaagcggaa tgcctacggt cttgcttcca gtttagtggc gagggtggac
    24541 ggatgacaac accataactg gcagggtatg cttatttccg cattttccga tgagggataa
    24601 agatgagtaa accacctctt ttctttattg ttatcattgg cttaatcatc gtcgccgcat
    24661 cgtttcgttt tatgcaacaa cgacgggaaa aagctgataa tgatatggca ccgctccagc
    24721 aaaagctggt ggtggtgagc aacaagcggg aaaaaccgat taacgatcgc cgttcgcgcc
    24781 agcaggaagt gacgccggca ggcaccagta tgcgctatga ggcaagcttc aaaccgcaaa
    24841 gcggaggaat ggagcagacg tttcgcctcg acgcccagca gtaccacgcc ctgacagtgg
    24901 gcgataaagg tacgctgagc tataaaggaa cgcgctttgt cagctttgta ggcgaacaat
    24961 aaggttattt tttaacttta aatttcttct gccagaccag cagttcaaac acgccgaaaa
    25021 ggaaaatccg caccttttcg gcggtggtca tctgcgggcc atctttcggt aaagtggatt
    25081 tcaatagcgc cagctgcata ccatgcatca gcacggtaaa aatcagcgcc acgttaacga
    25141 aaatattcag cgggcgtggg aagggatgca ccaggttgag gattaaaaat ccccaaacac
    25201 agagcattaa caaacgacca atattaatca gcatcacttt ctccttgtgc ttcgcgttga
    25261 tacagccgat aagccacctg acccgccact ttttcccgat gtaatgacca gtttgccgga
    25321 acagtgggca gacccttttc gacttcgctt tcgacataaa tcagggcttc gtcagccagc
    25381 cagccgttat cttccagtaa atttatcgtc tcttctaaca agccacggcg gaacggtgga
    25441 tcgacaaaca cgatattatg cggtgtgcct ttttgcgcca ggaatgacat cgcgttgctg
    25501 ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat tcttaattaa ctgctgagaa
    25561 accgcacgat ccatctcaat caacgtggcc cccgcagcgt agcgcgataa cgcttccagc
    25621 cccagcgcgc cgctcccggc gaagcaatcc agacattggg cgtcaacaat gaccggagcc
    25681 agccagttaa acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc tggactatca
    25741 ggaaccggga gtttacggcc tcgccactgc ccgccaataa tgcggatttg gccgctgccg
    25801 gaatgattcg gttttttcat gataaattgc tcaatccgcc agataacaca tacttgcagg
    25861 cggtgatgtg aattaagtta agtgatagac tatttcatca tttttttagc tgctatgtac
    25921 atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa tggcgaaaga
    25981 aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa aaagagcaga ccccggaaaa
    26041 agagacagaa gttcagaatg aacaaccagt tgtagaagaa atcgttcagg agcaagagcc
    26101 tgtgaaggct tctgaacaag ccgttgaaga gcagccgcag gcgcatactg aagccgaggc
    26161 ggaaactttt gctgccgacg ttgtggaagt tactgaacag gttgctgaaa gtgaaaaagc
    26221 gcagcctgaa gcggaagtcg ttgcacagcc ggaaccggtc gtagaagaaa cgccggagcc
    26281 agtggctatc gaacgtgaag aactgccgtt gccggaagac gtcaacgccg aagcggtttc
    26341 gccagaagag tggcaggccg aagcggaaac cgtagagatt gtcgaagcgg cggaagaaga
    26401 agcggctaaa gaagaaatca ccgacgaaga gccggaggca caggcgctgg ctgccgaagt
    26461 ggcagaagag gcggtaatgg tggtttctcc ggcagaagaa gatcagccgg tggaagaaat
    26521 cgctcaggag caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct
    26581 gttaaaaacc aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat
    26641 cgacgatgat ctgtttgaag agctggaaga acagctgttg atcgccgatg tgggcgtgga
    26701 aaccactcgt aaaattatca ccaatctgac agaaggcgca tcccgcaagc agcttcgtga
    26761 tgccgaggcg ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga
    26821 tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg
    26881 tgtgggtaaa accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc
    26941 ggtgatgctg gcggcaggcg atactttccg tgcggcagcg gttgaacagc ttcaggtctg
    27001 gggtctgcgc aacaatattc cggtgattgc ccagcatacc ggtgcggatt ccgcttctgt
    27061 tatcttcgat gccattcagg ccgctaaagc gcgtaacatc gacgtcctga ttgccgatac
    27121 cgccggacgc ctgcaaaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt
    27181 gatgaagaaa ctcgacgttg aagctccgca tgaagttatg ctgaccatcg atgccagcac
    27241 cgggcagaat gcggtaagcc aggccaaact gttccacgaa gccgttggct taaccggcat
    27301 cacgctaacg aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca
    27361 gtttggtatc cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt
    27421 taaggcggac gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt
    27481 gaacatgtca gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat
    27541 atgcagccgg gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc
    27601 ctgaagctga tctgtgggat tgagcggccc agcgccggga aaatctggtt tagcggccat
    27661 gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt
    27721 ttccaggatc accatctact gatggaccgt actgtctatg ataacgtggc gatcccgctg
    27781 attatcgccg gtgccagcgg tgatgacatt cgtcgccggg tgtcggcggc gctggataaa
    27841 gtcgggctac tggacaaagc gaagaacttc cctattcagc tttccggcgg tgaacaacag
    27901 cgtgttggca ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg
    27961 actggtaacc tggacgacgc gctgtcggaa ggcattttac gtctgtttga agagtttaac
    28021 cgcgttggag taaccgtatt gatggcaacg cacgacatca acctgatttc gcgacgttcc
    28081 tatcgcatgc tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg
    28141 cgatgcaatc aatcacattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt
    28201 cggcggctct ggcgacggcg gtcgtaacgc gccgaaacgc gcgaaatcct cgccaaaacc
    28261 ggtaaatcgc aaaaccaacg ttttcaacga acaggtacgc tatgcctccc acggcgcatt
    28321 gcaggatctg aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc
    28381 cctgacgctg cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca
    28441 atattatccg tcaccgcaaa tcactgttta tctgcaaaaa acgctggatg atgacgccgc
    28501 tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg gagaaagtga attatctttc
    28561 tcgtgaagac gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat
    28621 gctggaagaa aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg attttcaggg
    28681 gacggagtca ctgaatacgc tgcgtgatcg tatcacgcag attaacggca ttgacgaagt
    28741 gcggatggat gacagctggt ttgcccgcct ggcggcgttg accgggctgg tcgggcgcgt
    28801 ttcggcaatg atcggcgtat tgatggtggc ggcagtattc ctcgtcatcg gtaacagtgt
    28861 gcgcctgagt atctttgctc gccgtgattc tattaacgtg cagaaactga ttggtgcgac
    28921 ggatggtttc atcctgcgtc cgttcctgta cggtggcgca ttgctgggat tttctggcgc
    28981 attgttgtca ctaattttgt cagaaattct ggtgctgcgt ttgtcatcgg cggttgcgga
    29041 agtggcacag gttttcggaa cgaagtttga tatcaatggc ttatcgttcg atgaatgcct
    29101 gctattgctg ctggtatgct cgatgattgg ctgggtggca gcgtggcttg ccacggtaca
    29161 acatttacgc cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg
    29221 attccgtagc agggaaagag accccgttgt ctcttcccgg tattttatct ctatgtcaca
    29281 ttttgtgcgt aatttattca caaacttgca ttgaacttgt ggataaaatc acggtctgat
    29341 aaaacagtga atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga
    29401 agcgccagaa gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt
    29461 tagccccagt tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt
    29521 cggctgacga ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag
    29581 ctaaaacgct gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg
    29641 gctatggcct gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag
    29701 tgcgccgttt taacccggaa gtgggtgtgc gcctggtctc cttcgccgtt cactggatca
    29761 aagcagagat ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgaccacca
    29821 aagcgcaacg caaactgttc ttcaacctgc gtaaaaccaa gcagcgtctg ggctggttta
    29881 accaggatga agtcgagatg gtggcccgtg aactgggcgt aaccagcaaa gacgtacgtg
    29941 agatggaatc acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt
    30001 ccgacagcca gccgatggct ccggtgctct atctgcagga taaatcatct aactttgccg
    30061 acggcatcga agatgataac tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc
    30121 aaggtctgga cgagcgtagc caggatatca tccgcgcgcg ctggctggac gaagacaaca
    30181 agtccacgtt gcaggaactg gctgaccgtt acggtgtttc cgctgagcgt gtgcgtcagc
    30241 tggaaaagaa cgcgatgaaa aaattgcgcg ctgctattga agcgtaattt ccgctattaa
    30301 gcagagaacc ctggatgaga gtccggggtt tttgtttttt gggcctctac aataatcaat
    30361 tccccctccg gcaaaacgcc aatccccacg cagattgtta ataaactgtc aaaatagcta
    30421 ttccaatatc ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagc
    30481 tacgtataaa acgaaataaa gtgctgcaca acaacatcac aacacacgta ataaccagaa
    30541 gagtggggat tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc
    30601 tggcattcag caatatggct ctggcagaag atattaaagt cgccgtcgta ggcgcaatgt
    30661 ccggtccggt ggcgcagtac ggtgaccagg agtttactgg cgcagagcag gcggttgcgg
    30721 atatcaatgc taaagggggt atcaaaggca acaaactgca aatcgtaaaa tatgacgatg
    30781 cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat
    30841 acgtgattgg tcacctctgc tcttcatcaa cgcagccagc gtctgacatc tacgaagacg
    30901 aaggcatttt aatgatcacc ccagcggcaa ccgcaccaga gctgaccgcc cgtggctatc
    30961 agctgatcct gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata
    31021 ttcttgagaa agtgaaaccg caacgcattg ctatcgttca cgacaaacag cagtacggcg
    31081 aaggtctggc gcgtgcagtg caggacggcc tgaagaaagg caatgctaac gtggtgttct
    31141 ttgatggcat caccgccggg gaaaaagatt tctctaccct ggtggcgcgt ttgaagaaag
    31201 agaatatcga tttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc
    31261 aggcacgcgc ggcagggctg aaaactcagt ttatggggcc agaaggcgtg gctaacgtct
    31321 cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact
    31381 acgatcaggt tccggcgaac aaaccgattg ttgacgcgat caaagcgaaa aaacaggacc
    31441 caagcggcgc gttcgtctgg accacctacg ccgcgctgca atctttgcaa gcggggctga
    31501 atcagtctga cgatccggcg gagatcgcta aatacctgaa agcgaactcg gttgatacgg
    31561 tgatggggcc gcttagctgg gatgagaaag gcgatctgaa aggctttgag ttcggtgtgt
    31621 ttgactggca cgccaacggc acggcgactg atgcgaagta acagatagat aaatgaaaat
    31681 acccgatgcg ctgaactgcc atcgggtttt tttatattta tcgaaatggt tgtagaaaga
    31741 ttgaacaatt caggatatgc ttatgtacgg tgatatgccg tacatattat acggagcagg
    31801 agagttcaca tgtctgctgt taaaaagcag cgtattgatc tgcgtttaac tgacgatgac
    31861 aaaagtatga ttgaggaagc ggcagcgata tctaatcagt ccgtcagcca atttatgttg
    31921 aacagcgctt cgcagcgagc tgcggaagtg attgaacagc atcggcggat gattctcact
    31981 gaggaatcct ggacgcgggt gatggatgcg ctgagtaatc caccgtcacc aggtgaaaag
    32041 ctaaaacgtg cggcaaaacg tcttcaggga atgtaatact ttatggatga tctgacgata
    32101 gagattctga ccgatgatgc agattatgat ctacagcgat tcgactgcgg cgaggaagca
    32161 ttaaatctct ttctgacgac acatctggtt cgtcaacatc gcaataaaat tctgcgagcg
    32221 tatatcctgt gtcgcaacac tccagaacgt caggtgctgg gatattacac attatccggc
    32281 agttgttttg aacgagccgc actgccctcg aaatcgaaac agaaaaaaat tccctacaaa
    32341 aatattccca gcgtaactct tgggcgtctg gcaattgatc gttcattaca ggggcaggga
    32401 tggggagcaa cactggttgc tcatgccatg aaagttgtct ggtcagcctc tttagcggta
    32461 ggtattcacg gtctttttgt cgaggcgctg aatgaaaaag cccatacgtt ttatcagtcg
    32521 ctgggcttta tcccattggt cggagaaaac gaaaatgcgt tatttttccc gaccaaatct
    32581 atagagctac tttttacacc agacgattaa cgcttctccc agccaccctg ttgtgccgta
    32641 aatcccagtg ctcgcataaa cgccgtcatc acgccgcgat cttccacgcc agcatccgcc
    32701 atccaccaac atgaaacgcc aggattgtta cgcaaaacct cttccagcag atattgcccc
    32761 acaccgcgac gtcgggttac ttcccgcacg cgtagagaat ccagtgcccc ctcggtgccg
    32821 cttaaggtta cccgcacggc agcgagcagg cgctcgttaa aacgcgcggc gtagatgcgg
    32881 tggttatcgt caacctgtaa agaggaaggg gaatactccg gccagatctt ttgcaggtca
    32941 atccggtcct ggtcgctaaa gttttctaat cgaatgatgg tcagcttcat ggggaacccg
    33001 tgtaaatcac aaaagtggaa ccagtgtagc gaaataattt aatcggaggc tttctctttt
    33061 ttatttcttt tggcaggtga ttaatttttt ttcagcaata attacaaagt taaaacatta
    33121 gagaatgaaa aatatccagc ataatcccct aaatggtagt gaattattct gccctttgta
    33181 ccgttatttt atgctgacaa
//
LOCUS       DAPQZD010000041        33156 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPQZD010000041 DAPQZD010000000
VERSION     DAPQZD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.041"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..616
                     /locus_tag="RO454_001847"
     CDS             <1..616
                     /locus_tag="RO454_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0933690.1"
                     /translation="GYEFYIANYPEAGLYLAEMLCYPIDLIEKFQKFYAITDQIRNTT
                     GFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSL
                     ISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSA
                     IQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(773..1297)
                     /gene="tnpA"
                     /locus_tag="RO454_001848"
     CDS             complement(773..1297)
                     /gene="tnpA"
                     /locus_tag="RO454_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDY0933691.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            1320..1649
                     /locus_tag="RO454_001849"
     CDS             1320..1649
                     /locus_tag="RO454_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933692.1"
                     /translation="MKRLQAFKFRLRPGGQQERGMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            complement(1531..2028)
                     /locus_tag="RO454_001850"
     CDS             complement(1531..2028)
                     /locus_tag="RO454_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDY0933693.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(2137..2370)
                     /gene="yedF"
                     /locus_tag="RO454_001851"
     CDS             complement(2137..2370)
                     /gene="yedF"
                     /locus_tag="RO454_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDY0933694.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(2367..3572)
                     /gene="yedE"
                     /locus_tag="RO454_001852"
     CDS             complement(2367..3572)
                     /gene="yedE"
                     /locus_tag="RO454_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDY0933695.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            3759..4172
                     /locus_tag="RO454_001853"
     CDS             3759..4172
                     /locus_tag="RO454_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0933696.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(4206..5693)
                     /gene="amyA"
                     /locus_tag="RO454_001854"
     CDS             complement(4206..5693)
                     /gene="amyA"
                     /locus_tag="RO454_001854"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDY0933697.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(5771..6136)
                     /gene="fliT"
                     /locus_tag="RO454_001855"
     CDS             complement(5771..6136)
                     /gene="fliT"
                     /locus_tag="RO454_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDY0933698.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKIKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(6136..6546)
                     /gene="fliS"
                     /locus_tag="RO454_001856"
     CDS             complement(6136..6546)
                     /gene="fliS"
                     /locus_tag="RO454_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDY0933699.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(6562..7977)
                     /gene="fliD"
                     /locus_tag="RO454_001857"
     CDS             complement(6562..7977)
                     /gene="fliD"
                     /locus_tag="RO454_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDY0933700.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            8225..9274
                     /locus_tag="RO454_001858"
     CDS             8225..9274
                     /locus_tag="RO454_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDY0933701.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            9438..10157
                     /gene="fliA"
                     /locus_tag="RO454_001859"
     CDS             9438..10157
                     /gene="fliA"
                     /locus_tag="RO454_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDY0933702.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            10203..10754
                     /gene="fliZ"
                     /locus_tag="RO454_001860"
     CDS             10203..10754
                     /gene="fliZ"
                     /locus_tag="RO454_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDY0933703.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            10842..11642
                     /gene="tcyJ"
                     /locus_tag="RO454_001861"
     CDS             10842..11642
                     /gene="tcyJ"
                     /locus_tag="RO454_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0933704.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            11747..12733
                     /gene="dcyD"
                     /locus_tag="RO454_001862"
     CDS             11747..12733
                     /gene="dcyD"
                     /locus_tag="RO454_001862"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDY0933705.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEVAVNISSVVVASGSAGTHAGLAVGLEQLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            12748..13416
                     /gene="tcyL"
                     /locus_tag="RO454_001863"
     CDS             12748..13416
                     /gene="tcyL"
                     /locus_tag="RO454_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDY0933706.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            13413..14165
                     /gene="yecC"
                     /locus_tag="RO454_001864"
     CDS             13413..14165
                     /gene="yecC"
                     /locus_tag="RO454_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDY0933707.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            14395..15117
                     /gene="sdiA"
                     /locus_tag="RO454_001865"
     CDS             14395..15117
                     /gene="sdiA"
                     /locus_tag="RO454_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDY0933708.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(15185..15409)
                     /locus_tag="RO454_001866"
     CDS             complement(15185..15409)
                     /locus_tag="RO454_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDY0933709.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15396..15572)
                     /locus_tag="RO454_001867"
     CDS             complement(15396..15572)
                     /locus_tag="RO454_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933710.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            15868..16524
                     /gene="uvrY"
                     /locus_tag="RO454_001868"
     CDS             15868..16524
                     /gene="uvrY"
                     /locus_tag="RO454_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDY0933711.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16521..18353
                     /gene="uvrC"
                     /locus_tag="RO454_001869"
     CDS             16521..18353
                     /gene="uvrC"
                     /locus_tag="RO454_001869"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDY0933712.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            18410..18958
                     /gene="pgsA"
                     /locus_tag="RO454_001870"
     CDS             18410..18958
                     /gene="pgsA"
                     /locus_tag="RO454_001870"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDY0933713.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            19110..19185
                     /locus_tag="RO454_001871"
     tRNA            19110..19185
                     /locus_tag="RO454_001871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19143..19145,aa:Gly,seq:gcc)
     gene            19239..19312
                     /locus_tag="RO454_001872"
     tRNA            19239..19312
                     /locus_tag="RO454_001872"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19271..19273,aa:Cys,seq:gca)
     gene            19325..19411
                     /locus_tag="RO454_001873"
     tRNA            19325..19411
                     /locus_tag="RO454_001873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19359..19361,aa:Leu,seq:taa)
     gene            complement(19542..20558)
                     /locus_tag="RO454_001874"
     CDS             complement(19542..20558)
                     /locus_tag="RO454_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDY0933714.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALTEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKEK
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKTGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(20527..20790)
                     /locus_tag="RO454_001875"
     CDS             complement(20527..20790)
                     /locus_tag="RO454_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDY0933715.1"
                     /translation="MKSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFMNPIKFRNLQEVTTRKDDEPDFGAIFNGRKEKEPSR"
     gene            complement(21000..21182)
                     /locus_tag="RO454_001876"
     CDS             complement(21000..21182)
                     /locus_tag="RO454_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933716.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFTPQELITPNQK"
     gene            complement(21182..21751)
                     /locus_tag="RO454_001877"
     CDS             complement(21182..21751)
                     /locus_tag="RO454_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDY0933717.1"
                     /translation="MSTAIAIAKEYASLIDLLETADELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNANTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRIR"
     gene            complement(21748..23964)
                     /locus_tag="RO454_001878"
     CDS             complement(21748..23964)
                     /locus_tag="RO454_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933718.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIAAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKICEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVDSDCSSA
                     DISQVSDLMKLPARERFAAVLLFSHDANEVDSELLVQTREYLEMLDNSDTDSEDEVDA
                     FNRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPG
                     KRDEMLPEVKPETASARPYKQTHATLDREIACALLPVAPEKITPSILKAADEIISQDR
                     EDFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRD
                     ANAVEEKPKEAAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLR
                     EQFVTPRHVYDVPENNAVSQREPAAITLAEETPPQEQLSEQVKDLVQNVDALVERIHA
                     EEHKRQNAISAIETELKDSDDTDNLALWKNVFKTDERFTSAFSQNGGGTSINGTYIAM
                     KATREFGPFGIGWGVEVLEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTM
                     KVRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSAD
                     IFMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTANEV
                     NKVFGLIAREVDVHRKAAETKGDKEYSSYLGSRLRRITDIKTERLAALTAAQEQTA"
     gene            complement(23995..24315)
                     /locus_tag="RO454_001879"
     CDS             complement(23995..24315)
                     /locus_tag="RO454_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933719.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLLVATVDMSKNFPDRYILLES
                     REIEITVNQPEPIDIIGLQVEQLREQKQKTVADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            complement(25326..25739)
                     /locus_tag="RO454_001880"
     CDS             complement(25326..25739)
                     /locus_tag="RO454_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933720.1"
                     /translation="MNTKTKNMAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCN
                     GVTPRKDKLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRG
                     LPESEKNKLINELKEKREHFDLLLKELLEAKNQTK"
     gene            25838..26068
                     /locus_tag="RO454_001881"
     CDS             25838..26068
                     /locus_tag="RO454_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933721.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTEAA"
     gene            26127..26603
                     /locus_tag="RO454_001882"
     CDS             26127..26603
                     /locus_tag="RO454_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933722.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPELTIQEIDEAIAALVATRGAVIRTAQDHHA"
     gene            26643..26867
                     /locus_tag="RO454_001883"
     CDS             26643..26867
                     /locus_tag="RO454_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933723.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            26864..27619
                     /locus_tag="RO454_001884"
     CDS             26864..27619
                     /locus_tag="RO454_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDY0933724.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGVLLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEIITDGESADADTQQPADQRINYQV
                     ILDTYHEILPEMPPVKILTDGRKKHLRTFWKKFNFNESRWRAYLEFIGGNCRWMLEDR
                     PNGRGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            27609..29024
                     /locus_tag="RO454_001885"
     CDS             27609..29024
                     /locus_tag="RO454_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0933725.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIKGLFQADKPTDLITLTELMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMIQPADMTATDRITAVQQVIGQVAEHSRTGHKGG
                     LRPASEVVDDWIDDLERRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPNLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRLT
                     NDRSEKNKRYSRSDNSRTNEF"
     gene            29063..29473
                     /locus_tag="RO454_001886"
     CDS             29063..29473
                     /locus_tag="RO454_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933726.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAKPARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            29475..29711
                     /locus_tag="RO454_001887"
     CDS             29475..29711
                     /locus_tag="RO454_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933727.1"
                     /translation="MAGQSDYLPPGLPHNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYREFFRERLNYWRGVMEQ"
     gene            29708..30019
                     /locus_tag="RO454_001888"
     CDS             29708..30019
                     /locus_tag="RO454_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDY0933728.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSVKYLQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            30016..30240
                     /locus_tag="RO454_001889"
     CDS             30016..30240
                     /locus_tag="RO454_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933729.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            30922..31710
                     /locus_tag="RO454_001890"
     CDS             30922..31710
                     /locus_tag="RO454_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDY0933730.1"
                     /translation="MTVQISAESLSPLHHNQLPVITTELLASLYGTEVNGIQQNFKRN
                     IARFVVGKHFFKLEGNELRDFKNRLTDSQSVAKHTRSLILWTERGAARHAKMLETEQA
                     WEVFEKLEDCYFSQRPERRSTTTDDRTPLRDAVNILVGKKGMMYPDAYTMIHQRFAVS
                     HIEQLSQTQMLEAIEYIHCLLLDENHPTIPDFSFITTIKNGKVTGMRHVAKGEHLMTF
                     DAFKEIAERAGYLVIHSDNLRSMTLDKLMVMGKKSAISVANSAF"
     gene            complement(31885..32808)
                     /locus_tag="RO454_001891"
     CDS             complement(31885..32808)
                     /locus_tag="RO454_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HDY0933731.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDEIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLSANE
                     YVQLSDAYRKNFNRNLDKQMESLFLENNANAFSKRITYTVQATDDQKNVFVKTSTDNP
                     ISVGAALLTSLSIITPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQAKYEAEKQIER
                     QAQLQPQKADNLDQSRMAFANADSDLSTVWSKLTPAKKKDLLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
BASE COUNT         8268 a         7856 c         8416 g         8616 t
ORIGIN      
        1 tggatatgaa ttttatattg cgaattatcc agaagcaggt ctttatcttg cagaaatgct
       61 ttgttatccc attgatctaa ttgaaaagtt tcaaaaattt tatgcgataa ctgatcaaat
      121 tcgtaacacg acaggtttct gcttacctca gaaccccgag ttaatatatt gttgggagca
      181 actaaaaaca tctatttccc gaggcttctc aacgcaaatt caggaacact tagcaatggg
      241 cgttctactt tcattaggag cacatcatgt taattgttta cttttatcag atagtaaaca
      301 atcattaata agtcgttgtt ataacctaat gctatccgaa cctggaacaa aatggacagc
      361 aaacaaggta gcgagatatc tctacatttc tgtttccaca ttgcatcgcc gtctggcaag
      421 cgagggagta agttttcaaa gtatattgga cgacgtgagg ttaaataatg cgttgtctgc
      481 tatacaaacg acagtaaaac ccatcagcga gattgccagg gaaaatggtt acaagtgtcc
      541 ttctcgtttt actgaaaggt tccataatcg ttttaagata acaccaagag agctaagaaa
      601 agcgtccaga gagtaaaagt attttatgaa ggagcaacag catcgatttt tattttggaa
      661 aataaatacg gcatactcaa cctattggag tctgaacaat ccggtgactt ctgcgctaat
      721 cggggacgtt tctaagcaca tataattgat actatcttcc tccatcacaa tttcagaaat
      781 gcacgtgtga tttttttgca tccggcgttt ggactatgtg gagctcaaga gtgggaaagt
      841 tacaagaggt tgaggggata attaaaggga tgcttatgac attagatatc cggttaggta
      901 cggatatcgt tgttattaaa tctagttgat acgttaagat gcagctactg acaaaatagc
      961 caggactcca caaaacgcct ttgtcgtaat acctgactgc aatatctggt cgatctcgtc
     1021 gcagtaacct actcgatacg cctttgaggc tgtttaccag actggatatc gccagtttgg
     1081 gaggatagtt aattaacaaa tggacgtgat ctggtccgcc atccatttca acccgttcag
     1141 cttcaaaatc agcacataca tttgaaaagt aagtgcgtag tttttctgtc gcgtcatggt
     1201 caaaaatctg acgtcggtat ttagtaacga agaccaggtg aacatgcttc aggaaaacac
     1261 aatgccttcc gcgtcggata tcggtttctt ttttcatagg ccaaagtatg atttggccca
     1321 tgaaacgact acaggcattc aaattccggt taagaccagg tggtcaacag gagcgtggaa
     1381 tgaggcgctt cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggcg cttcagaatg
     1441 agaatcatga ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggttggttg
     1501 agtggaaaaa tgccactgaa acgcaatggc ttaaagattc gccctcacag ccattgcaac
     1561 agtcactgaa agaccctgag cgggcttaca aaaacttctt ccggcttcgt caccacgctc
     1621 aaacggtgtg ctacctatcg cggctataaa aacagcttgt ccatcagcac gatagataaa
     1681 ataaggctgc tttttgtcgc cttctttttt ccactcaaac cagccatcgg caaaacagat
     1741 tgcccgacca tgttgccaga gcggtttaaa catgtgactg gttgcggcag tttctacgcg
     1801 ggcgttaatc agcggcggtt tatcccacca tccgggagca tatccccaga aaaccggatc
     1861 cagatgaagg tgttcatcac gttcactgag cagaaggact ttggttcccg gcgcgacgtt
     1921 gtatctgcca ataggttcgg gatcgtaggg aatatcgcgt tcaatatctt ccgcgagaaa
     1981 cgcgaggtaa tcttcacgcg tttgggattg ggcaaagcgt ccacacatag aaacctcctg
     2041 agagcacact gaaaagaata ggagagaggg cgggtaaaat ctatgtgata gagaggtaac
     2101 gatgttcccg ctgtgggcca cagcgggaat gagggattac ttctgaatta aataacgaat
     2161 ggtcggcccg tcttgctgaa tatccagcac cgtatagccg tgattacgcg catccagtgg
     2221 aatattattg atcgactgcg gacagtcgct caccacttcc aggatctccc ctttttttaa
     2281 ctgcggcatc gcctcaaggg ttgcgactgc cggataaggg cagggttcac ccaccatatc
     2341 aagacggtaa tcaggaacga tatttttcat gcgatctcct tagcagtctg cggtgccgca
     2401 cggcggaaga agcgtttttc ccagccaata atcaacatta gagcagcaaa caacagcaaa
     2461 tatgtcacca gcaggccgcc catcgggcca aaggttttca gcaggttgat tttgtcccag
     2521 tcggtggcca gcgccggagc gaaatcatcc cagtagtacg ccagaatcgt tgagccgatc
     2581 acgttgccca gaccgaccca ccagtagtgc acctggcctt ctaccgcgcg gtacatccag
     2641 ccggtttcgc agccgcctgc cagcacgatg ccaaaaccaa acagtaaacc accaattacc
     2701 gcgtttggtc ccgcccacat gattttgggt tcaacgccta actgtacgta actgaagatc
     2761 ccgatggcac ttaccgccat accgataatg attgctttcg ccatatgggt acgtccggtg
     2821 atccacatat cgcggaacgc cgaagtaaag cagatttgcg cacgttcaat cagtaaacca
     2881 aagccgacgc caaacagcat tgccagcccc agtttgggct ggttcatcgc cgtcagcagc
     2941 gcccagccca gcatgccgaa aaagaccagc atcccgagac gaaaacgacg ccgcgcctga
     3001 tccggttttt gcgtcaacgg tgaggcagca gaaactttct gcattttcac gggaatacgg
     3061 aagatgggca gaagggtaaa gcgcgcgcca aaccatgaac caatggcagt ggcgatggca
     3121 aagaaccagg cgtgcagcga gaactgtgga attccggtaa agaacgccgc caggttacag
     3181 cccattgcca gacgtgcgcc aaaaccggcg ataataccgc caatgatggc ctgcataatg
     3241 cggatacggc tgcgcggcat gcgcagtttg acattgttgg cccacagcgc tgcggcaaaa
     3301 cagccgccaa acataccgag gatcatcatt ccgtcgatgc gggttaatgg cgatccttcc
     3361 agatggataa ttttaaaata accccactct tcagcatgga cgccgaacaa ctgcaggagc
     3421 tgaccgcccc aacgggtaaa ttcacccgtg actgcccaaa aggtgccagt gatgccaaaa
     3481 tagtaagtgg agagaatacc cgccgcgatg accgcgggga tgggcgccca gaatttaatc
     3541 aaccaggcgt gtttgaattg ctgccatgac atgaatgtag cctctgaact cagaaggttt
     3601 gaaacaagag tcgcgaatca tacaccatgc aaatttattt tcggggtaat aattcacaaa
     3661 aataatggtt agatcaaatt tctctctgaa cctggaaagg ctggcgacat cagggcttta
     3721 atgccacaat cctcttttct tctcatgcag gacgaatgat gaaaaaactc gcaattgcag
     3781 gtgcgctcat gttgctggcg ggctgtgccg aagtcgaaaa ctataacaat gtcgtgaaaa
     3841 cccccgcgcc ggactggctg acgggttact ggcagaccaa agggccgcaa cgtgcgttgg
     3901 tcagtccgga agcgataggc agcctcattg tcactaaaga gggcgatacc ctggattgcc
     3961 gtcagtggca gcgggtgatt gccgtgccgg gtaagttgac attgatgtct gatgatctca
     4021 ctaacgttac cgtaaaacgt gagctgtacg aggttgagcg tgacggtaac accattgagt
     4081 acgatggtat gacgatggag cgtgtggatc gcccgacggc agagtgtgcc gccgcgctgg
     4141 ataaagcgcc gttaccgacg ccactgccgt aatgaaatct ccccggcttg ccgccgggga
     4201 tggatttaaa tcacctcttc gataacccac acgctgacgc tgccgccgtt gcaaaagaag
     4261 gtcgcttcgc cgttttcgtc tgtcactacg ctctcttgcc ggttccccag aaaatcgcgc
     4321 caggttttat tgccgtaatt ctctcccaga tgaatggttt tttcgccatc atctccgttc
     4381 gacatgacca ccacgcagcc gggatattcg tcggtgccac tgcggctaaa ggcaatgcag
     4441 ttcggatggt cgaaaaataa cgtctgtaca ccgtgggcga aacgctgacg ggcgagaatt
     4501 aactcatcaa gctgttcgat tattggcata tctatcggat aggtttgccc gtcaccaccg
     4561 acatcttcgt aatgtgcacc gtagaggtcc ggatagaaca ccgaaggaac gccattttcc
     4621 cgcaacaaaa ttaaggcata cgccagcggt ttaaaccacg gttcgacggg ggcttcgagg
     4681 gcttgcaacg gttgggtgtc gtggttggca acgagggtta cggcgtgaaa aggatcggct
     4741 tccaccagcg tcccagtgaa gatctgcgtc atgtcgtagt cgcgacccat gcgcgatgct
     4801 tcatggaatt tcatctgcag cggcgcatca aacagcatgg ttttgccttc cacctgatca
     4861 atatacgttt gcagtttatc gacttcatgc gaccagtatt cagccacaat aaacaacggc
     4921 tttggcgcaa cttcctgtac gtgttcgatc cactctttat aaaaccatgc cggaatatgt
     4981 ttgaccgcat caagacgaaa gccgtcgcat tgcgtttgtt ccatcaccca gcgcgcccag
     5041 tatttaatct cttccgtcac ggcatgattg cgaaaatcga tattctcccc catcagataa
     5101 tcgaaattac cgagttcatc atcaacctga tcgttccagc cttctccggt atagtcgtta
     5161 acaattttaa aaatgccatc ttcatcaggg ttttcgatat ggtcgatacc gctaaaacat
     5221 ttgaaatccc agataaactg cgagtattgc ccggcacgag cggggaaggt gtaacgcgtc
     5281 cagccttcac actcaatgat ttcttcgtca atttgcgtac ggtcatcagc atttacacgc
     5341 tgcacgcgaa tagcttcttt ttcatccgcg cccattttgt ggttgactac cacgtccagc
     5401 agcaccgcaa tgtcattacg tttcagggcg tcaatggcgg ccagcagttg tgctttatca
     5461 ccatatttag tagggatgct gcctttctga tcaaactcac ctagatcaaa taaatcatag
     5521 gagtcgtagc cgaccgaata gccgcccgat gcgcctttat aggcgggcgg caaccagacc
     5581 atattgatac caatatcatt aaaaccgtct gcgcgctcgg ccagttcagg ccagagctta
     5641 ccgccttccg ggtaatacca gtgaaaacat tgtaacagcg tgggattacg cattcttcga
     5701 ctccattcaa ggggaatatt agaagcgtag ccgtaatcgg attattcgcg agccatcggc
     5761 tcattaagat tcaaaagagg ttatcctgcg gagccagcac aaagccgccc tgatcgccat
     5821 aggcacttaa caccgatttt tgcaccgatg actgaccgac cagtttcgcc agttcatcca
     5881 tccgaatctg taataactgc tttatcttgc tttcgttgtc gagaatcagg cgcagcatcg
     5941 ggcggagctg ctcctgcatc gtagtggacg gggcaacctc ttcagtcagg tgtgcgatct
     6001 cctgcaccgc attcacatac gccatttcgc tggcgatgag ttcgtcccat tgttcttccg
     6061 ttgccaggcg taacatgagc tggctttttt cgacgagttg ttgccaggcg aaatacaaat
     6121 gcggtgcatt gttcatcaga ctgggtcctg aatcaaagaa ggggagagta aagactcttt
     6181 ccaggcatcg gcaatattgc gcattaatgc ttccacttct tcgactgcgg agacatcgtt
     6241 gcgtaaattg gcttgcagca aacgcctgac catatagcta taaagagcaa tcaagttttg
     6301 ggttagttcg tctttgctct cttcatcaag actcacccgc agtccgttct caatgatgtt
     6361 gatcgctttt gacaaagaga cgcctttgcc ttgctgattg ttgtcctgca taaacaggcg
     6421 cgctctaacc agtgcgctca acactccatc aaatagcatg gtgaccaact gctgttggct
     6481 ggcgctcatt acggcgcttt cgacgccaat ttgtgcatag gcctgggtgc cttttgccgc
     6541 gtacatagtc tctcctgaag attatttcga gttgctattt gaggtgtcga actgctgtgt
     6601 cagatagcta ctagtggagt tcagcgagtt cattaacaca tctaattggg tgaactgggc
     6661 tttatagcgc gtcattaagg tatcgatacg ttcactggct gcgttgtact ggtcggtcaa
     6721 attattcagc gttttgctga cgccatcttt tgctgcctgg attatcccgg tggaagagag
     6781 ccaactggtc aggttgctac taatgccggt ggttacccca gtacttttgc cgtcacctat
     6841 aaacatttca ccgataccag cggcatcatt ttttagcgcc gtttgcagtt tgtcggtcgc
     6901 gatttccagc ttgccggtac tggcatcgct ggtaatgcca atctgtgcca gcgttttata
     6961 gtttgaactg ctgtgagtat tggcgagcaa ggttttaagc tgagtctgga tggtacgcag
     7021 tgtggagtca ccgagtaatg cgccattact ggagtcctga ctttcagctc cggcatcgac
     7081 agcggtgtat ttggttaagc tgctgaatgt atcctgcaat gtgttatagg catccaccca
     7141 cgctttaacc gcattttcag ctttggatgt gtctttactg atggtcagtg tctgattacc
     7201 ggtggtgacg tcattcaggt tgagggtgat atcttccagt gagtcgctga tagtgttact
     7261 gctgttttca atctcaacgt tattgactgt cagtttggcg ttttgcgcgg tgacgctttc
     7321 aatcatgcca ttgctgctat cacccggcgt tccgttgtaa cccataaaac tttccagggc
     7381 gctgtcgccg ctgacgctaa gtttcatagc gttattgctg cccgtatcgt tagcggtgat
     7441 ggacagacga tattcgccgt ttcccacatt gatgatactg gcgctgacac cagctttggc
     7501 gttgttaatg gcatcgcgaa tgccggaaag tgacgagtta cccgcgctga tatcaatcgt
     7561 gaccggatct ttaccgccgc cttgttggat ggtcagtacg ctatcgctgg tggcgatggc
     7621 ggctttactg tctttttgac tattttttgt tgtcagcgtc tgtgcctgcg ccagttggct
     7681 aacgctaatg gtgtatttcc cagcaatcgc gctgcccgtg gtggtggcgc tgaaagccga
     7741 tgaactgctg gtggttgagg tggcggtaaa taaatcggct ttattcagtg ctgtgttggc
     7801 tgtctggaaa ctctctagtg cacttttcag cgtcccgtag gcgctcagtt tcgcggtgta
     7861 agagctttgc tgttttgata tcggcgtcag ggtagatttt tctgccgcct ccagactgtc
     7921 aaggatgctg cttaagtcca gccccgaccc aaccccaagc gttgaaatac tagccatata
     7981 gtttccttaa tcatgcgacg tgtatagaac gaataccggg gttatcggcg taagcggggt
     8041 aaagtttacg atttattttt tgccttaatg gcacgaacag caacgaggaa ggggagtatt
     8101 tcgaccgcta gaaaaaaatt ctaaaggttg tgagtgacca gacgataaca gggttgacgg
     8161 cgacgaagcc gaagggtgga agcccaatac ttaaaccgta gacttgaaaa caggaaaatg
     8221 aattatggca caagtcatta ataccaacag cctctcgctg atcactcaga acaacatcaa
     8281 caaaaaccag tctgcgctgt cgacttctat cgagcgcctc tcttctggtc tgcgtattaa
     8341 cagcgctaaa gatgacgccg cgggccaggc gattgctaac cgctttactt ctaacatcaa
     8401 aggtctgact caggccgcac gtaacgccaa cgacggtatt tctctggcgc agacggctga
     8461 aggcgcgctg tcagagatta acaacaactt gcagcgtatt cgtgaactga ccgttcaggc
     8521 ctctaccggc acgaactctg attccgacct gtcttctatt caggacgaaa tcaaatcccg
     8581 tcttgatgaa attgaccgtg tatctggtca gacccagttc aacggtgtga acgtgctgtc
     8641 gaaaaacgat tcgatgaaga ttcagattgg tgccaatgat aaccagacga tcagcattgg
     8701 cttgcaacaa atcgacagta ccactttgaa tctgaaagga tttaccgtgt ccggcatggc
     8761 ggatttcagc gcggcgaaac tgacggctgc tgatggtaca gcaattgctg ctgcggatgt
     8821 caaggatgct gggggtaaac aagtcaattt actgtcttac actgacaccg cgtctaacag
     8881 tactaaatat gcggtcgttg attctgcaac cggtaaatac atggaagcca ctgtagtcat
     8941 taccggtacg gcggcggcgg taactgttgg tgcagcggaa gtggcgggag ccgctacagc
     9001 cgatccgtta aaagcactgg atgccgcaat cgctaaagtc gacaaattcc gctcctccct
     9061 cggtgccgtt caaaaccgtc tggattctgc ggtcaccaac ctgaacaaca ccaccaccaa
     9121 cctgtctgaa gcgcagtccc gtattcagga cgccgactat gcgaccgaag tgtccaacat
     9181 gtcgaaagcg cagattatcc agcaggcggg caactccgtg ctgtctaaag ccaaccaggt
     9241 accgcagcaa gttctgtctc tgttacaagg ctaatggcct taacctgcct gaccccgcca
     9301 acggcggggt tttttctgcc cgcaatttac cggtaacccc caaataaccc ctcatttcac
     9361 ccactaatcg tccgattaaa aaccctgcag aaacggataa tcatgccgat aactcatata
     9421 acgcagggct gtttatcgtg aattcactct ataccgctga aggtgtaatg gataaacact
     9481 cgctgtggca gcgttatgtc ccgctggtgc gtcacgaagc attgcgcctg caggttcgac
     9541 tgcccgcgag cgtggaactt gacgatctgc tacaggcggg cggcattggg ttacttaatg
     9601 ccgtcgaacg ctatgacgcc ctacaaggaa cggcatttac aacttacgca gtgcagcgta
     9661 tccgtggcgc tatgctggat gaacttcgca gccgtgactg ggtgccgcgc agcgtgcgac
     9721 gcaacgcgcg tgaagtggca caggcaatag ggcaactgga gcaggaactt ggccgcaacg
     9781 ccacggaaac tgaggtagcg gaacgtttag ggatcgatat tgccgattat cgccaaatgt
     9841 tgctcgacac caataacagc cagctcttct cctacgatga gtggcgcgaa gagcacggcg
     9901 atagcatcga actggttact gatgatcatc agcgagaaaa cccgctacaa caactgctgg
     9961 acaataatct gcgccagcgg gtgatggaag ccatcgaaac gttgccggag cgcgaaaaac
    10021 tggtattaac cctctattat caggaagagc tgaatctcaa agagattggt gcggtgctgg
    10081 aggtcgggga atcgcgggtc agtcagttac acagccaggc tattaaacgg ttacgcacta
    10141 aactgggtaa gttataacgt cagtaaatgc cgcactttaa ttttgactac caggagttct
    10201 taatgatggt gcagcacctg aaaagacggc cattaagccg ctatcttaaa gactttaaac
    10261 acagccagac ccattgcgcg cattgccgta aattactcga tcgcattacc ctcgttcgcg
    10321 acggcaaaat agtgaataaa atcgagattt cccgcctgga cacgctgctt gatgaaaatg
    10381 gctggcaagt ggaacaacaa tcatgggcgg cattgtgccg attttgcggt gatttacatt
    10441 gcaaaacgca gagtgatttt ttcgatatta tcggctttaa gcaatttctt tttgagcaaa
    10501 ctgaaatgag ccctggtacg gtgcgtgaat atgtcgttcg tctgcgccgt ttggggaatc
    10561 atctacacga gcaaaatatt tccctcgatc agctgcagga cggtttcctc gatgaaatcc
    10621 tcgccccgtg gctgccctcc accagcacca acaattaccg catcgcgtta cggaagtatc
    10681 aacactatca gcgtcaaacg tgtaccggac ttgtgcagaa atcctccagc ctgcctgctt
    10741 ctgatatata ttaaataaga ataagatgta gcggagttgt ttttgtgttt acaaacaatg
    10801 gctctacact gcaaacagac ataacaacat tcggggtgaa tatgaaatta gcacatctgg
    10861 gacgtcaggc attgatgggt gtgatggccg tggcgctggt tgcgggtatg agcgttaaaa
    10921 gttttgcaga tgaaggtctg cttaataaag tcaaagagcg cggcacgctg ctggtagggc
    10981 tggaaggaac ttatccgccg ttcagttttc agggggatga cggcaaatta accggttttg
    11041 aagtggaatt tgcccaacaa ctggcaaaac atcttggcgt tgaggcgtca ctaaaaccga
    11101 ccaaatggga cggtatgctg gcgtcgctgg actctaaacg cattgatgtg gtgattaacc
    11161 aggtcaccat ttctgacgag cgcaagaaaa aatacgattt ctctaccccg tacaccattt
    11221 ctggtattca ggcgctggtg aaaaaaggca acgaaggcac cattaaaacg gctgatgatc
    11281 tgaaaggcaa aaaagtgggt gtcggtctgg gcaccaacta tgaagagtgg ctgcgtcaga
    11341 atgttcaggg cgtcgatgtg cgtacctatg atgatgaccc gaccaaatat caggatctgc
    11401 gcgtaggccg tatcgatgcg atcctcgttg atcgtctggc ggcgctggat ctggtgaaga
    11461 aaaccaacga tacgctggca gtaaccggtg aagcattctc tcgtcaggag tctggcgtgg
    11521 cgctgcgtaa aggaaatgaa gacctgctga aagcggtgaa tgatgcaatt gcggaaatgc
    11581 aaaaagatgg cactttgcaa gccctttccg aaaaatggtt tggtgctgat gtgaccaaat
    11641 aatcagcata atgacaaaaa agggcgcttt cactagcgcc ttttttattt acgcgttttc
    11701 agcgtgcata ataagaaaat cacaacaaca cacatcggag gctgttatgc cactgcataa
    11761 tttaacccgt tttccacggc tggagtttat tggcgcgcca acgccgctcg aatatctgcc
    11821 gcgcttttct gattatcttg gacgggaaat tttcatcaaa cgggatgacg tcactcccat
    11881 ggcaatgggc ggcaataaat tacgtaagct ggaatttctc gcggcagatg ctctgcgtga
    11941 aggtgccgat acgctgatta ctgccggggc gatccagtct aaccatgtgc gccagactgc
    12001 cgcagtcgct gcgaaactcg gtctgcactg cgtggcgctg ctggaaaatc ctattggtac
    12061 aaccgcggaa aactatttaa ccaacggcaa tcgtttgttg ctggatctgt tcaataccca
    12121 gattgaaatg tgcgacgcac tgaccgatcc caatgcccaa ctggaagagc tggcgacgcg
    12181 agtcgaagca caaggctttc gcccgtatgt cattccggtt ggcggttcta atgctttggg
    12241 cgcgctgggt tatgtggaga gtgcgctgga aatcgcgcaa cagtgtgaag tggcggttaa
    12301 tatttcgtcg gtggtggtcg catcgggcag tgccggaact cacgccggac tggctgttgg
    12361 gctggaacag ctgatgcctg aaagcgaact gattggcgtg actgtgtcgc gttcagttgc
    12421 cgatcaattg ccgaaagtgg ttaacctcca acaggcgatt gcgaaagaac tggagctgac
    12481 cgcatcagcg gaaattttac tctgggatga ctattttgca cctggctacg gcgtgccgaa
    12541 cgacgaaggc atggaagcag tgaaattgct ggcgaggctg gaaggcattc tgcttgatcc
    12601 tgtgtatacc ggaaaagcga tggcggggct gattgacggt atcagtcaga aacgcttcaa
    12661 agatgaaggg ccgattttgt ttattcatac cggcggtgcg cctgcgctgt tcgcctatca
    12721 tccccacgtt tagctccggg aacgctcatg caagaaagta tacaactggt tattgattcc
    12781 ttgccgttcc tgctgaaagg ggctgggtat acgctgcaac tcagtatcgg aggcatgttt
    12841 tttggcttac tgctcgggtt tattctcgcg ctgatgcgcc tgtcgccaat ctggcccgtg
    12901 cgctggctgg cgcgttttta tatctctatt tttcgtggta cgccgctcat cgcacaactg
    12961 tttatgatct attacggact gccacagttt ggtatcgaat tagatcccat tccgtcagca
    13021 atgattggtc tgtcgctgaa cactgccgcc tatgctgctg aaacgctgcg cgccgccatt
    13081 tcttctattg ataaaggtca gtgggaagct gcagccagta ttggtatgac gccatggcag
    13141 accatgcgtc gcgcgatttt gccacaggcg gcgcgggtgg cgttgccgcc gctgtcgaac
    13201 agctttatca gcctggtaaa agatacgtcg ctggccgcga caatccaggt gccggagctg
    13261 ttccgtcagg cgcagttgat tacctcgcgc acgctggagg tgttcaccat gtacctggcg
    13321 gcttcgctca tctactggat catggcgact gtgttatcga cgttgcagaa ccattttgag
    13381 aatcaactta atcgccagga gagagaaccc aaatgagtgc cattgaagtt aagaacctgg
    13441 tgaaaaaatt ccacggtcag acggtgctgc atggtatcga ccttgaggtg aagccaggcg
    13501 aagtagtggc aattatcggt ccgagtggtt ccggcaaaac cacgttgcta cgcagcataa
    13561 atctgctgga acaaccggaa gcgggaacca tcaccgttgg cgatatcact attgatactg
    13621 cacgttcatt aagtcagcaa aaatcgctga ttcgccagtt gcgtcagcac gtcgggtttg
    13681 tcttccagaa ctttaatctg tttccgcatc gtacggtgct ggaaaacatt attgaagggc
    13741 cggtgatcgt caaaggcgaa ccgaaagaag aggccacggc gcgcgctcgc gagctgctgg
    13801 cgaaagttgg gctggccggt aaagaaacca gttatccacg tcgtttgtct ggcggtcaac
    13861 agcagcgcgt tgcgattgcg cgtgctctgg caatgcgtcc tgaagtgatt ttgtttgacg
    13921 agccaacgtc ggcgctggat ccagagctgg tgggcgaagt cctgaacacc attcgtcagc
    13981 tggcgcagga aaagcgcacg atggtgattg tgacgcacga aatgagcttt gcccgggatg
    14041 ttgcggaccg ggcgattttt atggaccagg ggcggatagt cgagcagggg gtagcaaaag
    14101 cgttatttgc tgatcctcag cagcctcgca cccgccagtt cctggagaag tttctgctgc
    14161 aataataaga aaaaatcagc cccgacgatt caccagtcgg ggcgggacgc catttcaaac
    14221 gtgataaaac tgcttaacaa atcagcataa ctcattaata acataagaga atgcgatggc
    14281 ttgcaaaagt aattcattgc ctgaataata taaattatat ataaatctta tttatgtgat
    14341 agtttgaatt atcattataa atgatactca ctctcagggg cgttgcggtt tactatgcag
    14401 gatacggatt ttttcagctg gcttcgcacg atgctgttgc gttttcagag gatggaggcc
    14461 gcagaagagg tctaccatga aatagagctt caggctcagc agctggagta cgattactat
    14521 tcgttatgtg ttcgccaccc ggtaccattc acgcgaccta aagtggcttt ctacaccaat
    14581 taccctgagt cgtgggttag ttattatcag gcaaaaaatt ttctcgcaat tgatccggtg
    14641 ctgaaccctg aaaactttag tcagggccat ttaatgtgga atgatgactt attcagcgaa
    14701 gcacagccgt tatgggaagc tgcgcgcgca catggtttac gccgcggtgt cactcagtat
    14761 ttaatgctgc caaatcgggc gttgggcttt ttgtcctttt cccgttgcag cacgcgcgaa
    14821 atacccattc ttagtgatga actgcaatta aaaatgcagc tactggtgcg cgaaagtctg
    14881 atggctctga tgcgtttaaa tgatgaaata gtgatgacgc cagagatgaa tttcagcaag
    14941 cgcgaaaaag aaattctgaa atggacggcg gaaggtaaga catcagcaga gatagcgatg
    15001 attttgtcaa tctctgagaa tacggtcaat ttccatcaga aaaacatgca gaaaaaaatc
    15061 aatgcaccga ataagacgca ggttgcctgt tacgcggccg ctactggctt aatttgatct
    15121 cttttctgtt ctgcgtgcca gatgcaaaaa ccggctgaaa ggcacgctct cagccggttt
    15181 tatattactg acggtaggct tgtttaattt gcttaaccgt tttggaaaat actgcagcct
    15241 gggtttcgtc ttcgatcagc gcaagctgtt tttccatctt taacatcacg cggcccgcgt
    15301 cagcttgtcc catcgcctgc agcatcagcg tcagcatcgc tttcaggcag gagacttcat
    15361 ttgccagttc ttgattattc tcggcagtag aaaaatcagg cgtactcatt tattttcctc
    15421 gtcatgttgc aatgaaaatt tgcggtgaaa aatgttaagg cggcggagta taccataagc
    15481 tttgctaaaa atagcagtgg ttgttttttg agcgtgatat caacagtact ataaaatacg
    15541 ttaatataat gaccaataaa tatttttatc atgaatgttt tttgcccgta ttgttcgggt
    15601 taattaatgt tacatattca gcgggctgat tttcattttt gctgaataaa gtcaattttt
    15661 gtcacatttc atcgccgggc ttactgtgaa acgatcccgg aagccgttgg tgacgggcgt
    15721 gaccataact gtggacaatc gaattgacaa aaacgagaga aaaatcgaat acccaccatt
    15781 tttaacgttt caaagttgca ataaaaaccg ctaatatacg aatgactaac tatcagtagc
    15841 gttatcccta tttctggaga tattcctttg atcaacgttc tacttgttga tgaccacgaa
    15901 ctggtgcgcg cagggatacg acgcattctg gaagatataa agggtataaa agtcgtcggt
    15961 gaggcatcgt gcggtgaaga cgccgttaaa tggtgccggg caaatgccgt tgacgtggtg
    16021 ctaatggaca tgagtatgcc gggcattggc ggtcttgagg cgacgcgtaa aatcgcgcgt
    16081 tccacagctg atgtcaaaat catcatgctt accgtccata cagaaaaccc tttaccagcg
    16141 aaagtcatgc aggccggagc tgcgggctac ctcagcaaag gcgcggctcc gcaggaagtc
    16201 gtgagtgcga ttcgttctgt ctattcaggg cagcgttaca ttgcttctga tatcgctcaa
    16261 caaatggcgt taagccagat cgaaccagaa aaaacagaaa gcccatttgc cagtttgtct
    16321 gaacgtgaat tgcagattat gctgatgatc accaagggcc agaaggtcaa tgagatctca
    16381 gaacagctca atctcagtcc gaaaacggtg aacagctacc gctatcgtat gttcagtaaa
    16441 ctaaacattc atggcgatgt tgagctgact cacctggcaa ttcgccatgg tctgtgtaat
    16501 gcggagacat tatcaagtca gtgagtgatc agtttgacgc aaaagcgttt ttaaaaaccg
    16561 taaccagcca gccaggcgtt tatcgcatgt atgatgctgg tggtacggtt atctatgtcg
    16621 gcaaagcgaa agacctgaaa aaacggcttt ccagctattt ccgtagcaac ctcgcttcgc
    16681 gcaaaaccga agcgctggtc gcccagatcc agcaaattga tgtaacggtt actcatacag
    16741 aaaccgaagc gctgttactg gaacacaact acatcaaact ttatcagccg cgttacaacg
    16801 ttttgctacg cgatgataaa tcatatcctt ttatcttcct gagtggcgat acccacccgc
    16861 gtctggcgat gcatcgtgga gcgaagcatg ccaaaggtga atatttcggc ccgttcccga
    16921 atggctatgc cgtacgtgaa acactggcgc tactgcaaaa gattttcccc attcgccagt
    16981 gcgaaaacag tgtttatcgc aaccgctcgc gtccgtgtct gcaatatcag atagggcgct
    17041 gtctggggcc gtgcgttgaa ggactggtga gtgaagaaga atacgctcag caggtcgagt
    17101 atgtgcgcct gtttttgtct ggcaaagatg atcaggtgct tacgcaactc attagtcgta
    17161 tggaaactgc cagccagaat ctggagtttg aagaagcggc gcggattcgc gaccaaattc
    17221 aggcggtgcg acgtgtcacc gaaaaacagt ttgtttccaa taccggcgac gaccttgacg
    17281 ttattggagt ggcgttcgat gcgggtatgg cttgtgtcca cgtattgttc attcgtcagg
    17341 gcaaagtgct cggcagccgc agctatttcc cgaaagtgcc tggcggtacg gaactgagcg
    17401 aggtggtgga aaccttcgta ggtcagttct atttacaagg cagccagatg cgcaccttac
    17461 cgggtgagat cctgctcgat tttaatctta gcgataaaac gctgctcgcc gattcccttt
    17521 cagaactggc gggacgcaag attaatgttc aaaccaaacc tcgtggcgat agagcgcgtt
    17581 atctgaaact cgcgcgtacc aatgcggcga cggccttaac cagcaaactt tcgcagcaat
    17641 ctaccgttca ccagcggctg accgcgcttg ccagcgtgtt gaaattgccg gaagtgaagc
    17701 ggatggagtg ctttgacatc agccatacca tgggtgaaca aaccgtcgct tcctgtgtgg
    17761 tgtttgatgc caacggcccg ctgcgtgcgg agtatcggcg ctataacatt actggcatca
    17821 cgccgggcga tgattacgcg gcgatgaatc aggtgctgcg tcggcgttat ggtaaagcta
    17881 tcgacgacag taagatcccg gatgtgatcc ttattgacgg cggcaaaggc cagcttgcgc
    17941 aggcgaaaaa tgttttcgcc gaactggatg tctcatggga taaaaatcat ccgctgctac
    18001 ttggcgttgc caaaggagca gatcgtaagg ctgggctgga aacgctgttc tttgagccgg
    18061 aaggtgaggg atttagtttg ccgccagatt ctcccgcgct gcatgttatc cagcatattc
    18121 gcgatgaatc acacgatcac gcgattggcg ggcaccgtaa aaaacgggcg aaggtcaaaa
    18181 ataccagttc cctggaaacc attgaaggcg tcgggccaaa acgtcggcaa atgttgttga
    18241 aatatatggg cggtttgcaa ggtttacgta acgccagcgt cgaggaaatt gcaaaagtgc
    18301 cgggtatttc gcaaggtctg gcagaaaaga tcttctggtc gttgaaacat tgatgtctct
    18361 gtagcaacat aggggtaatc ttactgacaa cagatagtta cccgtcatta tgcaatttaa
    18421 tatccctacg ttgcttacac tgttccgtgt catccttatc ccattctttg tattggtctt
    18481 ttatttgcct gtcacctggt cgccgtttgc cgccgcgctc attttctgcg tcgcggcggt
    18541 gactgactgg ttcgatggtt ttctggcacg ccgctggaat cagagtaccc ggtttggtgc
    18601 tttccttgac cctgtcgcag ataaagttct cgtggctatc gccatggtgc tggtaaccga
    18661 gcattaccac agctggtggg tgaccttacc ggcagcaacg atgatcgccc gtgaaattat
    18721 tatttctgcg ctacgcgaat ggatggcgga gttgggtaaa cgcagtagcg tggctgtctc
    18781 ctggattgga aaagtgaaaa ccactgccca gatggtggcg ttggcatggc tgctgtggcg
    18841 tccgaatatt tgggttgagt acgccggtat tgcacttttc tttgtggctg cggtactgac
    18901 tctgtggtca atgttgcaat atttgagcgc tgcgcgtgca gatttgcttg atcagtgatc
    18961 gtttcggcgc aattttcagc aaacgatcaa aagtggtgaa aaatatcgtt gactcatcgc
    19021 gccaggtaag tagaatgcaa cgcatcgaac ggcggcactg attgccagac gataataaaa
    19081 tcaagtgatt aactgattgc ttgatgaatg cgggaatagc tcagttggta gagcacgacc
    19141 ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccagttta aaaggcatcg
    19201 gcgtcagcgg atgtctggct gaaaggcctg aagaatttgg cgcgttaaca aagcggttat
    19261 gtagcggatt gcaaatccgt ctagtccggt tcgactccgg aacgcgcctc cacttttttc
    19321 ccgagcccgg atggtggaat cggtagacac aagggattta aaatccctcg gcgttcgcgc
    19381 tgtgcgggtt caagtcccgc tccgggtacc atgggaaaga taagaataaa atcaaagcaa
    19441 taagcagtgt cgtgaaacca ccttcgggtg gtttttttgt atcttctttc tcatttccga
    19501 agatgcgtcc gaagatgatt tttctctgga cgcatcatca ttcattttct ctggccacca
    19561 actacgggca ctactttaac tttccggtca tatgctgctg tctgtcgcgg gttcttatgc
    19621 ccggaaattg cttgcttctc ttccaggctg ccttccagat cagagatacc ttttgctttc
    19681 agatcatgaa atgtgaagtc tatttgcaga tgaggatatt tttcctgtgc tgccgctttg
    19741 acatcacgcc aacgagagtt aaaaccatca cgggtatact tgccaccggt agtttgatga
    19801 atcacaaaca ggctactgat tcccgttttt aatggcagag aacgagccag agctatcgct
    19861 ttctgcagac gaggcgacca ggctttgatt tgcttaacgc cggtttttcc ctgacgaatg
    19921 tagatcccgg aatcgaacag ctggtctttc tgcaatgaaa gtacatcact ctgtctggcc
    19981 acgcataagt aggcaatctc cattgctacg cgaacaacat caggagacac ttcataaacg
    20041 gcctgatatt cttcatccgt aatataacgt tcgcgagctt tctctttaaa ctgtttaact
    20101 ccctgacaag gattcctctg aacataacct cgttcataac cccaacggaa tacccgggac
    20161 ataaagcttt tttccctgtt tgcctgagtt cggcttgata aaccgcgttg atccatatag
    20221 cgccgaatat gttcaggttt gattttatcc gggtcgatct tcccgaagac aggcagaact
    20281 tttccggaat attttgtgta gtctttccgg gtttcgactg ccaaatccat aaaatcaggg
    20341 gaggtcatga attgttccgt gagcgcctga aaagtatttc tttctttttc gtcaccgacc
    20401 gctttttcat atgccagcca tacggctgat cgtggtgcat ccaatgcgca caggcgtatc
    20461 gctttgttat ctttattcct gaattcgtag gccgctttgc cctggtaaac acgaggcggc
    20521 atccagttat ctgctgggtt ctttctcttt ccggccatta aatattgctc caaaatcagg
    20581 ttcatcatct tttcgcgtcg tcacctcctg taaattgcga aattttattg ggttcatgaa
    20641 atggccccag gttgtttttg ggtgtccgtc agccctttcc atgaagaaaa ttccagcccg
    20701 gcgaagagcc tcacattgct ttgatttaag tgggcttccc gtcagctcga ccatttcttc
    20761 tctggtgatt atgtcgtgat cgcttttcat ggtctttcct cattatgtag cttattgcgt
    20821 catctgcctt ctggcaggca cgggcaatat cagactcggt caaagtctgt ttcctgacgc
    20881 tggccgacag tcgaccaatt ttgatatcaa atgcggagag cagagttgct cccggttgcc
    20941 atcgcagcat tgtgatcctc cggtgtctgg tgaatcgcaa tgctagcgat ggtgagtttt
    21001 tatttctgat taggcgtaat cagttcttgt ggggtgaatg ccgatttctc ccgagtgacc
    21061 ttaatgtttt ctggcaggtg tagcccaagt tcgcagcggc tccgtgcttc aataatgcca
    21121 ttggtaccat cggcaagtac caggtgaacc gcgtcaccac gctttaatgt gagtttaagc
    21181 attagcgtat cctcagtgac cgttcgccgc gttccagatg tgcgcctggt accgggttta
    21241 ataactcggc tggtggtgtt tctccacgcg ccagtatttc ggcggcaacc gcctccgcag
    21301 attcaatcac ttctttaatg gctcgcttgt ttggtgtaat aaccgtttct acatcaacaa
    21361 gtgacacgcc ctcatactca tccgggatct tatctttgct atcgataatt acccggatag
    21421 ccccctgtgc atcagtaaat gtgttttttg cggttttcag tttatccagg ccagcagctt
    21481 gcaggcaggt gagcatatgc tttcgaattg ctttttcctt gccttcaaag gacgtcttac
    21541 gggttgccag gcgagacatt tcttcagcgc aggtattggc attgccctgt aaattacggc
    21601 agatgaccat catggcgtcc agtttgtctc ccagttctcc ttccatgcct tcaagcgtgt
    21661 cggcgatcat ctctggcgtc agttcatcgg cggtttctaa caggtcaatc agactggcat
    21721 attcttttgc aatagcgata gcagtgctca tgctgtttgc tcctgtgcag cggtaagggc
    21781 ggcgaggcgt tcagttttaa tgtcggtgat acggcgcaga cgtgatccca gatacgatga
    21841 atattcttta tcgcctttgg tttccgctgc tttcctgtgt acatccactt cgcgggcgat
    21901 gaggccaaat accttgttta cttcgttggc ggttaccgcg gcggccagcg tattggcaac
    21961 gttgataagc ttctcatcca gttccttacg cagacgggta gcatcttccg cattctcgct
    22021 ggcattcttg atatcaaact cagctttatt cctctggcgg tattccggat tgtcgtacag
    22081 gcccatgaaa atatcggcgc tgaatccgag aggggatagc gctttcttaa cagcatccgt
    22141 ccaggatttc ttcggtgctt cgccgtcgct tgttggtccg tatttagtgt cgtagatata
    22201 aggtgtgcat ccgtaagcct cggactcacc gcgtacccca ttcaatatgt accagactct
    22261 cactttcatg gtgtggtgca tttcacatag gtacccgcca acaccgtctt gaatcagatc
    22321 ccattctatt ttttcaccaa cctgtttttt gcgaactatt ggtgcgcctt tatcaaagcg
    22381 ctcctccagc acctctaccc cccagccaat accaaatggc ccaaattcgc gagtcgcttt
    22441 catcgctata taagtgccgt tgatggatgt gccgccgcca ttctgagaga aagcactggt
    22501 gaagcgctca tcggttttga atacgttctt ccacaatgcc aggttatccg tgtcatctga
    22561 gtcctttaac tccgtttcga tagctgagat tgcattctgc cgcttgtgct cttcggcatg
    22621 gatccgctca accagcgcgt caacgttctg caccagatcc tttacctgct cgctaagttg
    22681 ttcttgtgga ggagtctctt ctgccagggt aatagctgca ggttccctct gtgatacggc
    22741 gttattttca ggtacgtcat acacatggcg aggcgtgaca aactgctcac gcaactgctg
    22801 caaactacgt tctccagcat ttgcagcttc gcttttttcg ccctgatttg agggtgtttc
    22861 tgacatcatc ccatcaattg agaatcgtcc gccgccgtgg ttggtgactt ttacatcatc
    22921 cttgggtgct gcttcctttg gcttctcttc aacagcattt gcatcacgct gcccgttagc
    22981 ttgcagccag ttgtcgatgt gactgcgcag ggattgtggg aagtggtacg tgtcttttgc
    23041 gggggcgctc tgaataacac cgaatactga tgcgcggtca taggcaagga tctgatccgt
    23101 tgtgcgcaag gccattgacc agcgtttaaa atcttccctg tcctgactga ttatttcgtc
    23161 tgccgctttc aggatgctgg gggtgatttt ttccggggca acaggtaaca gggcgcaggc
    23221 aatttcacga tccagagtgg cgtgagtctg tttgtaagga cgtgcggatg ctgtttctgg
    23281 ttttacctcc ggcagcattt catctcgttt accgggattc tctacccatt tttttataaa
    23341 tttggagata gtccaactgg tcgggttttg ttcctgggta tcgcaacttg ccaaaattgc
    23401 atgtaccaga ttattcaatc cagcaatatg catgtactcg atgggcttac acgccaccat
    23461 ggcatcaaga acgatgcggt taaacgcgtc aacctcatcc tcactgtcag tatcactgtt
    23521 atcgagcatc tccaggtatt cacgcgtctg cacaagcaat tcactgtcga cttcgttggc
    23581 gtcgtggctg aacagcagaa cggcggcgaa tcgttcacga gccggcagtt tcatcaggtc
    23641 gctaacctgt gagatatctg ctgaactgca atctgaatca acaggagtgt taactaccca
    23701 tttttcgcca tcaaaactgt gttctgtggc aaacgtctca tcaaactcac cgacgcctgg
    23761 gcgcggttgg cccggggcat cttcgcagat tttaggtttg aaatagttgt cgccgtggtc
    23821 agggtaggct tcccacaatt tgccgatgat gatgttctcg gcaacttttt tgttaggggc
    23881 ggcgatggcg atggccagcg ccgggatcgc gccgtttttg agcgcgcctt ttttcgcttc
    23941 taacaagccg ttaaaaatgg tcattggtct ttcctcatta caggtcagtg gtttttagta
    24001 ggggagctca tcggtatctg gcgtgtattc gatgcaaagt aactgctgga ttttgtcatc
    24061 gatagcagca atacgctgtt gggcgtcggc gactgttttc tgtttttgtt cacgcagttg
    24121 ctcgacctgt aaaccgatga tgtcgattgg ctcgggctgg ttaacggtaa tttcaatttc
    24181 gcggctttcc agaaggatgt acctgtctgg aaaatttttt gacatatcaa cagtcgcaac
    24241 aagcagcttt tcgtggctga aagttgaagt tgcataatga ataaagagtg ttactgggat
    24301 ggtgcgcgct tccatagcga ctccttggta atgtatactc agagccgatc agcgaatatt
    24361 gatcagcttc catagcgtca gtgtcgttgg tctttcctca tcacaagttt ggtcacttgt
    24421 gataaatccg aatggtttgg tcgccgttcg gggtaactgg cccgccttgt gcgggtcttt
    24481 tgctatctaa agggtgccgg ttacgtttcc ggcgtcgtta tgtttccatt acgaccgtac
    24541 agggctggtc aggcccgggc tggtctttcc tgttttgagc tggtcaggct catggtcaac
    24601 gctggtcagg cgtggtactt cctccggtct ttccctggtg tcacgctgcg ggtttggcct
    24661 ctcctgttgg ggactcaggc acagcgataa aacctggctg tgtgaaaaaa tggcccatcg
    24721 tgcggactgg gcaaagacta aacacagcaa attgatgatg ggcgcccgga atcgaaccgg
    24781 gtaacgggca gggagttccc gttaaacacc tgttcaccac aaccgggagc gcactccgcc
    24841 atttcaaaat ttaacgacaa agctcaaagt tgagtcgatg aagtgcgctc tcgtgttgta
    24901 gccaggactc ttccctggtg gtcacaccgt atcgccttga tggtgaatca atcgctcata
    24961 cctggctgtg gcttgcacat tccggctacc tgctatggag acaacgcgtt aaggctccgc
    25021 ttccatccag accgcttcga cacatgtgcc atatgccggt gaagtctttc cctctgtcac
    25081 cgtgttggcg ccgacgcgca aaatttggtg ccgtctttcc ggctgtcaga acgtgtttct
    25141 gaacaactgc cgctgggtaa gcgttgttga tgaaagtgac tttacaattt gtatttgtaa
    25201 tggtcaatgt gtttttaaaa aataatttgt agtgaggggc aaaaaaatat tcactgtgta
    25261 aaggtgtttt tgtgttaggg gaaagttgca tgccagcagc ggctggcctg gaaatttgaa
    25321 ctgaactatt ttgtctggtt tttggcttca agtagttctt ttagcagtag atcgaagtgc
    25381 tctctttttt cttttagctc attgattaat ttattttttt cgctctcagg cagtcctctg
    25441 aatagttgca gtagagcctg ttcttgcggg gttagttctt gattgtaatt acccgggtgt
    25501 agtgtggatt catccaaata ttccccttca ttcatgaaga aaaaatgaac agggtatccg
    25561 gtcacttttg ctagcttgtc taatttatct tttcttggag ttaccccatt acaccatgcc
    25621 tgaactgatt gagccgtaac cccaatatgg cgagctaact cagattgaga ccagcctaag
    25681 tcttttagaa tccgttgtag tcggctagca aaagccatgt ttttcgtttt tgtgttcata
    25741 ccgtaaggat acaaggttta attgtaggga gcattgcaaa tataatttgt aattacagtt
    25801 attgtttgta atgatgacgc ggacacaagg agtccatatg gataaacaac tgcaagacaa
    25861 aattctttcc gtaatgagtc aatccgagtt agggcggcga ttagggaaaa aaccacaaac
    25921 ggtaagcctc tggttcaaag gacgggttcc cggtgaagag gttttgcgca catctgaagc
    25981 tcttgaatgg cgcgtgaccc ctcatgatct tagaccggat ctttacccaa acccagatga
    26041 cagcttgcct aaaacggaag cagcttaact gttggtgagc cccaaatctg attaagcgta
    26101 atcaattttt agcgacagga gacgctatgg aaaaccaaga ggaattacaa aaagagattt
    26161 tgacctgggc ggcaagggca gggcaggaac tcgtcacgat tgaaatctgc cgggcctggt
    26221 tcagtcaggg acgcaatgat gagttgagac tacatgaatt tgaggacgcg gacggcaacg
    26281 tggactggag agccatcaac aacaatcggc agaaaatctt tcgctggtta cgtggcgaga
    26341 caacggcggc gcgccgaaaa actcaggtgc tggccagtgt gatgaaagcc gtgctacccg
    26401 cagaacggcg ggcacgtctg gagtcgccgg gcgatcccgt tttgctggca acgctggcgg
    26461 caaaagaagg ggtggaagcg attaacgctg tacatctcca cttcgcgcca gagttaacta
    26521 ttcaggaaat tgacgaagca atagcggcgc tggtagcgac gcgaggagca gtgatacgca
    26581 ctgcgcaaga ccaccatgca tgagcgcctg accagcgttt aacttatacc gaggaaagac
    26641 cgatgttaag acatattgac cgcattacct ggcgtaacgg ctggcacctg aatggacgcc
    26701 cggcacatgt tgcagagatc cgccccatat tcgatggccg cgtcgcagct gcacgttctg
    26761 tatgggaaaa gtacgaagaa gaaaaagcga aactgcgtga gcaaaatctc tctggcgctg
    26821 cctatgaggc tggctgtcgc gttctctcag aggctttggg catatgaaca tcctcccgtt
    26881 acttgataga cccatagcct tccagagaag ttttatccgt cttgaaatgg gcgtgactgc
    26941 cgcattgttt ctgtcgcagt tgacgtactg gacaaacaga acaactgacg acggctgggt
    27001 atataaaacc caggatgagt gggaagagga aacagggctt tcacgatacg agcaggaggg
    27061 cgcgcgcaaa aaactacgtg gtctcggcgt cttgctggaa cgtaaacagg gcttgcctgc
    27121 caggctgtac tacaaaatcg ataatgatgt gttgtgccag ctacttacgt ccgcatacaa
    27181 ggatgcggaa aaaccacata caggtgaggg gaaaaccacc aggcctgtac gtggaaaacc
    27241 aacaaacatt cttacagaga atactacaga gattattact gatggtgaat cggctgacgc
    27301 cgacacccaa caacctgccg accagagaat aaattatcag gtgatactcg atacctatca
    27361 cgagattttg ccagagatgc caccagtgaa aatcctcact gacggcagaa aaaaacacct
    27421 tcgcactttc tggaaaaaat tcaatttcaa tgagtcccgt tggcgggcct atctggaatt
    27481 tatcggtggc aattgtcgct ggatgctgga ggacaggcca aatggcaggg gaggattctg
    27541 gcggcgtaaa aatcttgatt atctgattac cgaacgctgc tatgtttcgg tcaaggaaga
    27601 acgcgcaaat gataaataac gcgattgcac tgtacagcgt tgacgcagaa caggctgttc
    27661 tgggctgtct gatgctgaat accgaccacg acagaaccaa tgcggtattt gcgttactga
    27721 aaccggaaac gttttatatc aatgcgcatc aggttatcta ccgggagatc aaaggattat
    27781 ttcaagctga taaaccaact gacctgataa cactgaccga actgatggaa tcgaaggggc
    27841 tgtatgagcg cgtgggggga ttcgcatacc tggctgaaat gagtaaggcg gcgattccgg
    27901 cctcaatggt caactacgcg aaaattgtac gtgaaaaagc cattcttcgt tatgccgtgg
    27961 aaaagttgaa ttcttgtctg gagattatga tccagcctgc agatatgact gctactgaca
    28021 ggatcaccgc cgttcagcaa gtgattgggc aggtggccga acattcccgt accgggcata
    28081 agggcggttt gcgtccggca agtgaagtcg tcgatgactg gatagatgac cttgagcgcc
    28141 gtttcaatga tccagcacat gcagccggac taacgctcgg aatcgaaagc cttgatcgct
    28201 taatggcacc aaagcaggcg ttaagaggtt cattggtcgt tattggtgcc cggccaaaaa
    28261 tgggcaagac agccgcgttt aataaaattg cggtgcattt tgctctgaat catcgtttgc
    28321 cgacgctggt atttagcctg gaaatgactg acagaagtct cattgaacgc atggttgcgc
    28381 aggaagcaaa ggtcaattcg gagatttttt atcttggccc taacgatgaa tctgaattga
    28441 gtctcgcaat agcaaaggcg ggggagattg ccgaatccaa catgatgatt gacagcacgg
    28501 caggtgtaac ccttgcgcat attgtcgctg aatgtcgaaa agttaaacgg atgcgtggct
    28561 cggttggcct ggtggcaatt gactatttga cgctgatgaa gaccgaatcg gcagagcgta
    28621 gggatattgc gtatggcgat atcacaaccg gtttgaagaa cctggcaaaa gagctggatt
    28681 gtgttgtggt cctgctgacg caactcaacc gaaagcttga agaacgtgcc gataagcggc
    28741 ctaacccgag cgacagtaaa gataccgggc agatcgaaca agattgcgat gtctggattg
    28801 gcctctatcg tgatgctgtt tataacgaaa atgccgaccc aaatctcatg gaaatgttgc
    28861 tacgtctgaa ccgcgaagga ccatcgggaa cagcctatgc cctgatgaaa aatggctcag
    28921 ttattgatat cagcgatgag gaggtttatc gccttaccaa cgaccgctca gaaaaaaata
    28981 aacgttattc ccgtagcgat aattctcgca caaatgaatt ttaaccacgc ctgaccagcg
    29041 tgaaataacc gaggaaagac caatgaccac cactccaggc aaattagagt acccgtcgga
    29101 caaaggacac atagacgacg gtaaaaacta tctcgacgtc attttgtgga atatgaacgt
    29161 cgggccacgt gcgcgcactc gcgcggtttt tgtgccaaga cctaaagcag gcaatttttc
    29221 aacccctgct aagcctgccc gtcaggcatc cgtagccgca gcgccagcag tgaaacgtaa
    29281 gggcaaaacg cacaccggga tcgcgattcg cagaaatggt gagcggcaag tgaaactcca
    29341 cgaaacagcg acaacctggt gtgcctcacc gcatgaaact tacgacaaga taaccgggca
    29401 gcgcattggc gcgcctggtc gctgccgcct gctgctgagt tccatcactc cgatagcaaa
    29461 gaaaggggcg tgatatggcc gggcaatcag attacctgcc gcccggctta ccacacaacc
    29521 gcgccaaatg gccccaggaa taccagctta aagagcacta cgacatgcga gcggcggcgc
    29581 tgatccgcca actctttgag aaacgtattc cgaggggaag cgtgattgag caaatcggaa
    29641 tgacgccaga tacgtaccgg gaatttttca gagagcgtct gaactactgg agaggggtga
    29701 tggaacaatg aaatacaaac ggtgggtgcg tgcggaagtc gtaatcatca aacaatgtgc
    29761 gggcagcatg acagttgaac gtatagggca gcttattggc agaaccggtg cagcggtacg
    29821 cacaaaagcg cgcgagctga aaatctgtat gtatctgcgg ggtaattatc accagtcagt
    29881 gaaatacctc caggaagata tcgagctggc aagggaatta catcagtcgg gtattaatcg
    29941 ccaggatatc gcagaaaaac tcgaaatgcc gatcggagcg gtgaatcagt ttgtttattt
    30001 tgagcggaga atttcatgaa agaacttttt cttgcatttg tgccccggtt tattaatgac
    30061 cagatcgcac tgactgataa tggtgaacaa tatgaaattg cctgcagcat ggtggatgtg
    30121 aatcctggcg aacggtatga cgcgatgtgt gacctgaaaa tatttacctg gctgggttgg
    30181 gctattccgt gtggagaacc aaccaatatt cgtccgtttg agagcaggga ggctgtatga
    30241 gtgaattgaa aatacgtcgc aaggcaatag cacgcgttaa aaatctatta ccagtgcctg
    30301 tcaaatatcg tttttgtggt gggaatgtac ggattggtac ggatgtatgg tgattgcgga
    30361 gtgacgtaaa acgaatcgtt gtgggtgtaa tatcgtcaat atcattggtt tcccggaggg
    30421 aaaggacatg tttggcgata aactggagtt accacggaac gggcggatag tgctctatat
    30481 ttcagattac aaagtaaccc gggctgaggt gttggaaccg gaaaaacatc tggtcacgtt
    30541 accggacatt attgagttgt ataagcgggt cggatatgtg gacttgcatc gcgatgatgt
    30601 tcccgttgag tgtatggaat gtgaacaccg gaggtttgaa gttgattgaa ttgacttaga
    30661 aaaagtccgg ggctatagtt tccgcgcagc cgcaaaatcg gttgccggga ttggcgtccc
    30721 ggatatctaa gtgacgcata gccgcgttag cggttttttt atgtgttagg cacggccaca
    30781 ttcgcattat ggtgggctgt gtgggggctt ctgtggaagc gccgggttca cttagccggt
    30841 tacgccaacc ctgcacagct caccaccaga tgattggcgt cgctggtggt gatgaaaaaa
    30901 acactaagtg aggccactgc gatgacagtc caaatatctg cggaatccct ttctccgcta
    30961 catcataacc aacttcccgt catcactact gaattgcttg ccagtttgta tggcacggaa
    31021 gtaaatggta tccagcaaaa cttcaagcgc aacatcgcca gatttgtcgt aggaaagcat
    31081 ttttttaagc tggaaggtaa tgaattacgc gacttcaaaa acagactgac agatagtcag
    31141 tcagttgcta aacatacccg atccctcatt ctctggacag aacgcggcgc agctcgccat
    31201 gccaaaatgc tggaaacaga acaggcctgg gaagttttcg aaaagctgga agattgttat
    31261 tttagccagc gtccagagag gcggagtaca actaccgatg accgtactcc cttgcgtgat
    31321 gccgttaata tactggtcgg caagaaaggc atgatgtatc ccgatgccta caccatgatt
    31381 catcaacgct tcgccgtctc tcacatagaa caactgtcac agacgcaaat gttagaggcg
    31441 attgaataca tccactgcct gctactcgat gaaaatcacc caaccattcc agatttttct
    31501 tttatcacca ctatcaaaaa tggaaaagtg accgggatgc gccatgttgc caaaggcgaa
    31561 catctgatga cctttgacgc tttcaaagag atcgctgagc gggcagggta tctggtgatc
    31621 cacagcgaca atctgcgaag tatgacactg gataaattga tggtaatggg gaaaaagtcg
    31681 gctatatccg ttgctaattc agcattttga caggttgttt ggggcggaaa tactctattt
    31741 gcgcttaaaa aggtatttga ccacgaccag cacgagaccg aatacctctg tcaaagtacc
    31801 gcccatataa aacagttaca tatgcacttg atgactaagc tggcaccagg ttaacacctg
    31861 gcgccagctt gaaggagact tcagttattg gtttctgagg aaagcgattc ttgaaagggt
    31921 catttgcgtc tggcagtcga ccattttctt gacttcagca tctgttccct gcattgaaac
    31981 tttaccgcac atagcatcct tagttttgat ccactgacgc tgagaaggca gcaaatcttt
    32041 tttcttcgcc ggggttaact tgctccagac ggtactcaaa tcagagtcgg cattcgcaaa
    32101 tgccattcgg gactgatcaa ggttgtcagc tttttgcggc tgaagttgtg cctgtctctc
    32161 tatttgcttc tccgcctcat acttagcctg ttcttgcgcc ctgagttgca cctgttgttc
    32221 cgcttcaatt tgactctgtt gggcatcctt agcctgctgg attttctgct gctcaacgat
    32281 cggggtgata atggaaagcg atgtcagtaa agcggcgccc actgatatcg gattatcagt
    32341 agaggttttt acaaaaacgt ttttctgatc gtcggtcgcc tgcacggtgt aggtgatacg
    32401 ttttgaaaag gcgtttgcgt tgttctccaa aaacaggctt tccatttgct tatcgagatt
    32461 acgattaaaa ttctttctgt aagcatctga aagctggacg tactcgttcg cagatagagt
    32521 catcgtcacg gtcccttcac aagttttcat tgtgctgccc gtgtcacttg atgttgtgga
    32581 gatctcagag atggctagtt tgatcttgtc cagtgtgctt cgtttggtct gattagtgac
    32641 atcagggtat ttatcgacct gttcagagag tccttcataa gcagattttt ttaataaatc
    32701 catgagagct gactgggtta tttccgaaga acaaccgatc tcatcttttt tgttatcaca
    32761 gcctgtaagg gcaatggcga gtaagagtac tgagtatttt aatctcatga attcccttta
    32821 gagtaaggat tggtttttaa gacgcggtct gtaatcagtc ccgagagcta cagtaaacaa
    32881 cgctgagcac tgaacgcatc acaggtaagg taaaaggtga tatcggcaaa ggctgtgaaa
    32941 cctttaattc aacagatggg aacggtatgc gcaggatcct gtaacgacgc tcccaaaacc
    33001 ggaaacctgt aaggcatgtt ttcgctgctg aacttaatat acgaacctgc actatcatga
    33061 acatatttta tatcgttata ccccgagtga ttgtgcccgg caatggatag tgtcctggag
    33121 gaactatttc tactgtaaaa ggtagatatt taattt
//
LOCUS       DAPQZD010000042        33142 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPQZD010000042 DAPQZD010000000
VERSION     DAPQZD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.042"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            79..1410
                     /gene="argA"
                     /locus_tag="RO454_001892"
     CDS             79..1410
                     /gene="argA"
                     /locus_tag="RO454_001892"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDY0933732.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQTRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(1492..3318)
                     /gene="recD"
                     /locus_tag="RO454_001893"
     CDS             complement(1492..3318)
                     /gene="recD"
                     /locus_tag="RO454_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDY0933733.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEVLAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(3318..6860)
                     /gene="recB"
                     /locus_tag="RO454_001894"
     CDS             complement(3318..6860)
                     /gene="recB"
                     /locus_tag="RO454_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDY0933734.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(6853..9741)
                     /gene="ptrA"
                     /locus_tag="RO454_001895"
     CDS             complement(6853..9741)
                     /gene="ptrA"
                     /locus_tag="RO454_001895"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDY0933735.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(9917..13285)
                     /gene="recC"
                     /locus_tag="RO454_001896"
     CDS             complement(9917..13285)
                     /gene="recC"
                     /locus_tag="RO454_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDY0933736.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(13298..13621)
                     /locus_tag="RO454_001897"
     CDS             complement(13298..13621)
                     /locus_tag="RO454_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDY0933737.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(13606..14013)
                     /locus_tag="RO454_001898"
     CDS             complement(13606..14013)
                     /locus_tag="RO454_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDY0933738.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(14010..14573)
                     /locus_tag="RO454_001899"
     CDS             complement(14010..14573)
                     /locus_tag="RO454_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDY0933739.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(14564..15034)
                     /locus_tag="RO454_001900"
     CDS             complement(14564..15034)
                     /locus_tag="RO454_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDY0933740.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(15219..16013)
                     /gene="thyA"
                     /locus_tag="RO454_001901"
     CDS             complement(15219..16013)
                     /gene="thyA"
                     /locus_tag="RO454_001901"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDY0933741.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(16020..16895)
                     /gene="lgt"
                     /locus_tag="RO454_001902"
     CDS             complement(16020..16895)
                     /gene="lgt"
                     /locus_tag="RO454_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDY0933742.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(17046..19292)
                     /gene="ptsP"
                     /locus_tag="RO454_001903"
     CDS             complement(17046..19292)
                     /gene="ptsP"
                     /locus_tag="RO454_001903"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDY0933743.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(19305..19835)
                     /gene="rppH"
                     /locus_tag="RO454_001904"
     CDS             complement(19305..19835)
                     /gene="rppH"
                     /locus_tag="RO454_001904"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDY0933744.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            20520..21209
                     /gene="mutH"
                     /locus_tag="RO454_001905"
     CDS             20520..21209
                     /gene="mutH"
                     /locus_tag="RO454_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDY0933745.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            21278..21991
                     /locus_tag="RO454_001906"
     CDS             21278..21991
                     /locus_tag="RO454_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDY0933746.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            22129..22347
                     /gene="ygdR"
                     /locus_tag="RO454_001907"
     CDS             22129..22347
                     /gene="ygdR"
                     /locus_tag="RO454_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDY0933747.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            22455..23495
                     /locus_tag="RO454_001908"
     CDS             22455..23495
                     /locus_tag="RO454_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDY0933748.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(23527..24720)
                     /gene="lplT"
                     /locus_tag="RO454_001909"
     CDS             complement(23527..24720)
                     /gene="lplT"
                     /locus_tag="RO454_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDY0933749.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(24713..26872)
                     /gene="aas"
                     /locus_tag="RO454_001910"
     CDS             complement(24713..26872)
                     /gene="aas"
                     /locus_tag="RO454_001910"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDY0933750.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            27458..28489
                     /gene="galR"
                     /locus_tag="RO454_001911"
     CDS             27458..28489
                     /gene="galR"
                     /locus_tag="RO454_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDY0933751.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(28496..29758)
                     /gene="lysA"
                     /locus_tag="RO454_001912"
     CDS             complement(28496..29758)
                     /gene="lysA"
                     /locus_tag="RO454_001912"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDY0933752.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            29880..30815
                     /gene="lysR"
                     /locus_tag="RO454_001913"
     CDS             29880..30815
                     /gene="lysR"
                     /locus_tag="RO454_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDY0933753.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(30802..31494)
                     /gene="ygeA"
                     /locus_tag="RO454_001914"
     CDS             complement(30802..31494)
                     /gene="ygeA"
                     /locus_tag="RO454_001914"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDY0933754.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(31623..33041)
                     /gene="araE"
                     /locus_tag="RO454_001915"
     CDS             complement(31623..33041)
                     /gene="araE"
                     /locus_tag="RO454_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDY0933755.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8161 a         9097 c         8208 g         7676 t
ORIGIN      
        1 taaaatttac acttgcaccc tgcgaaaaaa cagaataaaa atacaataat ttagaataat
       61 catgcaaaga ggtgtaccgt ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc
      121 cattcggttc cctatatcaa tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt
      181 gaagccattg agcatgagaa tttctccagt atcgttaatg atatcgggtt gttgcacagc
      241 ctcggcatcc gtctggtggt ggtctatggc gcacgtccgc agatcgacgc aaatctggct
      301 gcacatcacc acgaaccgct gtatcacaag aatatacgtg tgaccgacgc caaaacactg
      361 gaactggtga agcaggctgc gggaacattg caactggata ttactgctcg cctgtcgatg
      421 agtctcaata acacaccgct gcagggcgcg catatcaacg tcgtcagtgg taattttatt
      481 attgcccagc cgctgggcgt cgatgacggc gtggattact gtcatagcgg gcgtatccgg
      541 cggattgatg aagacgcgat ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg
      601 ccggtcgctg tttcggtcac tggcgagagc tttaatctga cctcggaaga gattgccact
      661 caactggcca tcaaactgaa agctgaaaaa atgattggtt tttgctcttc ccagggcgtc
      721 actaatgacg acggtgatat tgtctccgaa cttttcccta acgaagcgca aacgcgggta
      781 gaagcccagg aagagaaagg cgattacaac tccggtacgg tgcgcttttt gcgtggcgca
      841 gtgaaagcct gccgcagcgg cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc
      901 gcgctattgc aagagttgtt ctcacgcgac ggtatcggta cgcagattgt gatggaaagc
      961 gccgagcaga ttcgtcgcgc aacaatcaac gatattggcg gtattctgga gttgattcgc
     1021 ccgctggagc aacaaggtat tctggtacgc cgttctcgcg agcagctgga gatggaaatc
     1081 gacaaattca ccattattca gcgcgataac acgactattg cctgcgccgc gctctatccg
     1141 ttcccggaag agaagattgg ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt
     1201 tcatcacggg gcgaggttct gctggaacgc attgccgctc aggcgaagca gagcggctta
     1261 agcaaattgt ttgtgctgac cacgcgcagt attcactggt tccaggaacg tggatttacc
     1321 ccagtggata ttgatttact acccgagagc aaaaagcagt tgtacaacta ccagcgtaaa
     1381 tccaaagtgt tgatggcgga tttgggttaa gcgatgcaaa tcgccggatg cgacatgcac
     1441 aacattcata ggtatgataa gacccacatg cgtcgcatca ggcaattacg tttatccccg
     1501 tgaactaaac aacgccgcca gaccactgcg ccgctcggtg cgcgtggcaa ttgccgcact
     1561 taaaatgcgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca ccgcggtgta
     1621 aaccagctct cgcgttacta ccggcgtgcg ttgactcggt aaaatcaacg ccgcatggtc
     1681 gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtcgtttcgt gctctggcag
     1741 gcgactcggt tgcacagact taatattgcc gtccggcatc gcaaaccaga cgcgcgtccc
     1801 ctgcccgcga tccagcgcaa taccaatatc gccattaaac aacccaagcg cgctgtcatt
     1861 acgggcaatc atcactggtc gaccttcgta ccaacgagag tgcggatgac gatgaatttt
     1921 gcgcttctgt tgcataaatt gctcaattcg ctcattcaat ccagccacgc caaacggtcc
     1981 ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc tgaatgatta aatccggctc
     2041 ggcacgcgct tgcagcagat ccagataacg tccgtaaccc gcaagaactt cctcaagcat
     2101 cgcaatataa tcttcgccac tctgtaaaag ccgtttttcg atatcagtaa aatcctgctg
     2161 aaaaacggtt ttcactgccg ttttatcacc tcggttaatc gccgcagcta actgaccaat
     2221 gccagaatcg ctgccgaaac gatagctttt ttgcagcagg cagaggctgt cgcgcaaaga
     2281 tgccgcttct gtgccagttc ctgctggaac gtggcttccc gtcaggcggc tcaactgccc
     2341 ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag atatcgccca gcacagcccc
     2401 agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg catgatcggg
     2461 caaggcgtcg atcagtctcg acatcatagg aagatcgatc attgacgctt catctaccac
     2521 cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc tacccggctg
     2581 cgcgcccagc aatcggtgca aagtgctggc atcttccgga atgcgtttct tttgttcagc
     2641 ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg cggcagcttt
     2701 acccgttggt gcagccagac ggatacggca gcgttctccg tcggccattt gaattaaggc
     2761 tgccagcaac ttcgctacgg tggtcgtttt accggtgcca ggaccgccgg aaatcaccga
     2821 aatccgccgc gtcagcgcca ctgccgccgc aactttttgc cagttaattt catcgcttac
     2881 tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa tggcatgatt
     2941 cacttcgttg aaaaaacgtg ccactgtgcg ctcgttacac cacatgcgat tcaaataaag
     3001 acgatcgcca cagaggatca tcggcgttgg ttcatctccc cggctgaccg cttgtgaagc
     3061 cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg tcgccaacag
     3121 cggatgcgac gcctcgttat tttccagtcg tgaaagcggc aaacaaacgt gtccctctcc
     3181 ggcatcatga cttaatagtg ccgccgcgag ggtgacggca ggatgttcat ctcccgccac
     3241 ggtcagggca aactgcacgt ccagcgggcg tagctgtttg tgctccacag cttccagtaa
     3301 ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat ctcatccatc
     3361 aggtcaatca acccggcgtt gggtcgggtc gcgtagatcc cttgttgcgg atgttcttta
     3421 tcaacgccac gcaggaacag ataaataacg ccgccaaagt ggcgctcata gtcgtaatca
     3481 gcaatacgat ggcgcagata acggtgcagc gccagggtat aaagctgata ttgcagatca
     3541 tagcggtgtg cctgcattgc cgctgccata gcctgttggg tgtaagccga actgtcttca
     3601 cccaaccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg gaacaccaga
     3661 tcgataaagc cttttaacat gccacgtacc tgcatgaact ccagcggcgg gcagccagca
     3721 gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag cggttcgcta
     3781 attggcaggt aaaactccat ctccacctgt ttatcgcgat cggaaagctg actcaggcta
     3841 atacccgttt cattgagagg tgccaggagg acagccgtga tccactcagt caacaccggt
     3901 tcccactgcg gttcaaagcc accgagctcc agtttttcct gtacccagtt cgggtcaacc
     3961 ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg tgacgcaccg
     4021 cgcgggaact gatgcggtgt taacgtcggt acttcaacga cgctgaccac gcctgcggca
     4081 tcaacatcca accgtggcat cagatcctga gcgataccgt gaccacgctg ctgcaaaccg
     4141 gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagcgtcct cgcattcagt
     4201 tctgcagtta atgcatcatt aacctgccag ggctggttat caccaatttg tgccgtttgc
     4261 caggcaatat catcatcgca caacgcttca atgcaggtac gaagccctgc cacatcttgc
     4321 ggttctcctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt atcacctttt
     4381 ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca aaccgaacgc
     4441 gtcagcgcca cgtaaagtaa acgcagatct tccgccagac gttcggcctc cgcgaggtcg
     4501 acgctttctg gcgcagcatt aagatccaga acggcctcaa acgagtggcg atcgtgataa
     4561 aacgcctgat cctggacgcg gaaattggta ataaatggca accagaccag cggatattcc
     4621 agcccttttg atttgtgtat cgtgacaatc tgcaccagat gtttatcact ttcgaggcgc
     4681 atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa ccagcgtacc
     4741 agcgcatgtt cactttccag ctgcgttccg gcttcttgta gcagttcgct gatatgcaag
     4801 atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttctgc aatgttacgc
     4861 gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat ttgccgataa
     4921 ccatcgaact cttcgactac cgcatcccac gcatgttcgt cattattcag cgtttcaata
     4981 tccagcgcat tcagccccat cattgacgtt gccagcgcac tgcgcagggt gttctcacgt
     5041 tcgggcgtca tcaccgcctg caacagccaa agcatctcct gtgcttccag agtttcaaaa
     5101 acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcagcgttaa ggcatcgcgc
     5161 acctgagcgg cctcctggcg gctgcgcacc agcacactga tgtccgaagc acgcaccgga
     5221 cgcgcatcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg tagccagtcg
     5281 cggatttgcg cagcacatac ctgcgccatg gtactttgat agtcgccaac gccgcagctt
     5341 tcgccttcca tcagccacat tttcatcgca ggctgcattt cacctttaaa cacaaagcgt
     5401 aacgcctgat ttttcccggc agatttcaca ggaataaacg gtatttcacg aaacatgaac
     5461 gcgtcatcag tctggctgaa aagtttgtta acgctgttca ccattcccgg tgcggaacgc
     5521 cagttggtat ctaaggtgta gtgagcatga acttcgctac gcgccttcat ataagtgaag
     5581 atatccgcac cccggaatgc atatatggcc tgtttcgggt cgccaatcag caacaatgcg
     5641 gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg gtcggtatcc
     5701 tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc caatacctcg
     5761 ccgctttcac tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa acccaattcg
     5821 ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc gcgggtgatc
     5881 accagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc aaacagtgga
     5941 tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta agaaacgctg agagaatttt
     6001 tccagcgact ccggcaactg atagctattg gtctcttctt ctgcccaggc gctgatcttc
     6061 tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc agaagattcg
     6121 atcagcgcat ccagttcacc cactgcgtcg cgccactgct gttttacggc atcaatacgc
     6181 gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cggggggcgg tgctttgata
     6241 actggcgctt cgccttgcag ataacgatta atatcgcgca gcaacgcctg cggccctttc
     6301 caggtttcaa agacgacctg ggcaatttca cgcggcagcg ggtagcagtg gcgacgccag
     6361 aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg ctgctcaaac
     6421 agcatgccgg attcaaaggc attcaggttg agcatgcgct ggcaaaagcc gtggatagta
     6481 aagactgccg cttcatccat ctgccgctcg gctaacaaca accactgcgc ggcttgcgct
     6541 ttatcgtcga tctcttccag caggcgtttg tacagtggat tgtcggtggt ttcacgcaga
     6601 caggcgatgc gcaactcgtg gatattgcta cggatacgac cacgcaattc tgccgtggca
     6661 gcctcggtaa aggtcaccac cagcagttct tcaacggtca gcgggcgggg aaaggcggcc
     6721 gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa ggttttgcct
     6781 gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa aggatctagt
     6841 gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct gcaacgcgct
     6901 gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc cattctggct
     6961 gccggaaatc tgcgacagaa tagccatacc ttgcggctcg accaccgcct gatggaagaa
     7021 atcagcaatt ttttgcggcg tcagcagttt tatctgggcc acgattttat cacgcgaatc
     7081 gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt cgccgagcgt
     7141 ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa actcttctgg
     7201 cttcatcgcc cgcaattttg cctctgcggt tgggaaaaac gccttgtaac gctcccacaa
     7261 gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact gacgccccac
     7321 gctcattgga aacgcaaaca cggcatagcc caactgttct tcggtacgca actgattgta
     7381 gaaccacggc tgtacgatct gccccaacag agaactatac gctgagctgg tgtattcatc
     7441 gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac cggctttttc
     7501 aaagatgacg gattgttttt tatcgaccac gacatctttg ttacgacacc actctgaacc
     7561 atcagcgccc aactgttttt gcacatggcg tgccagcgtt attgcctggg cttcagtcat
     7621 gttgccgata accataaact ctggtctagc ccctgatttt aaggcgtcgc gataggccag
     7681 cacctctttc aacgtaatgg agggcaaaat tttgcgccgt tcatctcgcg agaagtacgg
     7741 cacttgcgag agcatctgcg cgggcataat cgcctgctcg aacgctttgc ccttttctgc
     7801 ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt ccgtagcggt
     7861 atagctaaag tagccctcca gcagagcctg gaacagctgc ggcagacgct gggtgtaacc
     7921 attagcatta accataaggc cgttgttagc attggtggaa aaacttatgc caccaaccga
     7981 cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga gtgcaaacat
     8041 cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc tgacatcagc
     8101 tttgggctcg ctggcaaaat aacggcttgg cgcatacacc acgcgcagat tcgactcatc
     8161 aacaatcaac tctggatggt cgtatttctt ctctgactta atcagcgaga aatcgtcagg
     8221 aatatagggg ttaagctccg gtaaggagag cgcaatattg gcggcttttt tctgccagtc
     8281 agcgaaagtt tgtgcgctaa ttttatcgac ctgatacggc gcatcgacaa agtaagccgt
     8341 tttgttgtgc ggctctttcg ggctgatata ccagatacgc gcattctgtg gcgtcatcat
     8401 cgccagacgt tcttttaccg ctttagcgtc gtaccgatcg gcaatattga ctgcatccag
     8461 tgtatgctca acaggaacgc gaatcatggt atctgccagc cattcgacgt aatccatatc
     8521 acgggtgatt gacggataac ggaagtcgat atccagcaca ttcgccagtt catcgaagta
     8581 ttgtttatcg atcccttttt cacgtaacag attgaggtag ctaaaaatgg ccgccacaac
     8641 ctgatcgcga ttcgccagac ctttatcggt taaagacgca gagatcgcta atacgccgct
     8701 gttgccgttg acgataggat cggaattggc gctaatgccc tcaactaatc cctgcttttg
     8761 cagccagtca gaaagtgtac cagggctgcg attaccaatc agataggtaa tcaattcatc
     8821 cgttttacta cggaacttcg ctgaattgtt atcgatgcga aactcaacgc gcagaacttt
     8881 acgcggcagc gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg tgactaccgg
     8941 cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg tgtccgccgc
     9001 cattttcgcc aactccggca acggtttatt gctgtaaata accgccttca tcagattggc
     9061 ggaatagtac ttctcgtgga aatctttcag cgcctgctgt accggattac ctggtttgtc
     9121 gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg ggttaatggt
     9181 ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg ttaattcggc
     9241 attcactgca ttacgctcac gttcggcgta tttcttgtcg agcaagggtt ctgcaatagc
     9301 atcggccagg cggtctaccg caccgggtaa ggcatcgttc tcaacttcca gatagaaagc
     9361 cgtgcgatac ggtgccgtgc tggcattgtg actgccgccg tgcattttga gatattcggc
     9421 cagactgtca gcctgcggat actttttcga ccccatcaga ctcatatgtt caaggtaatg
     9481 tgccagcccc tggtacgcct caggatcttc cagcgaccca acgggcacca ccagcgccga
     9541 gagcgattta actgcctgcg gatcagaaac cagtaagacc accataccgt tatccagacg
     9601 tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct gaatcggctg
     9661 ccatcccgtt tctgcctgac ttaagggtgc ccagagggca acaaacaaca ataatgcttt
     9721 gaaccagatg ctgcggggca ttcacggacc tcataatcaa ctttattttc tggccagatt
     9781 caacacgtta acgcaaatca tctgccagaa tttaatcttg tgctgaacga gtcagcctat
     9841 gtctatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga tagattgcgc
     9901 aatttttata caacactcat gactgattaa agcgaaacag cggtaacagg aaacgttgcg
     9961 actgttcaac gatagcctcc attgtctctg gtgttaattg ccgccagagc cgttgatacc
    10021 agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga aggaatttcg
    10081 tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc gcgtcataac
    10141 aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg gacattcctt
    10201 cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct gcaagcggcg
    10261 gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta ccaccgctgg
    10321 cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt aataaagagg
    10381 gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagcccgtt atttgcacac
    10441 cgttgcaggc gagatcaatc tccatactct gccccggctg gcgacaggca atgactctgt
    10501 cggcaagctg ctgcatctcc tgacactgcg tttcccagaa aatttcacca aaagcgccat
    10561 acggtaaatc ccctgccgcc cggaagcgac ggaacaagcg ttcggcatca tcctgctcaa
    10621 ccagtgcatt caataactgc tgattgattt gataacggct aagtccttcc agaataaatg
    10681 gctcggtgtc ggggatttcg ctgtcttcgg tacggaagtt cacctgcaaa cgcatctgga
    10741 aaaatgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc acggtttccg
    10801 gtaaggtaaa tggcagcggc tgaacaaatt cagaatgtgc tttaccagcc tggctggccg
    10861 caggtagcca ttcacgagca taactttgtc gttcgcctgg ctggtagttt tgtggatcaa
    10921 acggcattcg ggtatggagg caagtaagat gcgcttttac ccttgcctcg ctttcatcac
    10981 agttgagcgc ttcatcgccc ggtagataat ggctttgccc gatgtagtcg atcagttcct
    11041 gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg atatagctga
    11101 tatagagttt ttgctgcgcg gaaattaacg cttccaggaa cagatagcgg tcgtcatcgc
    11161 gacggctacg gtcgccacgc ttcggtttct ggctcatcag atcaaagccc aatggcgcaa
    11221 gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg aacggaattg
    11281 aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc tggctgatac
    11341 gttcttgatc caggcgctgt gccagttcat cacgcaatag tgacagcggc accgcgtcgc
    11401 catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg atcagcgtca
    11461 tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg cgacaaaccg
    11521 gcaaccactc ttccagcgga cgctcctgcg ccagcccgcg acgccagata tttagctgca
    11581 ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat tcatcataag
    11641 gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc aacatgcgcg
    11701 tcaggccaaa ccgccaggtg tgttgaccgg tagcgggaag ttccagctcg cgaacgttgt
    11761 cgtcatctat cccccaacga atgccggact cgttgaccca ctggcgtaaa taacgcagcc
    11821 cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagtaat gccagcacgt
    11881 cttccgacac aaagcggctg tctggcaacg ataacaggct gataaacgct tcaagtacag
    11941 gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg caggtaacga tccgcaggtg
    12001 cgctaccaaa cacagcctga ataaacggac tgtagctgtc gatatcagcc accatcacga
    12061 tgatgtcgcg cggagtaagt gtcgggtctt cctccagcat cgccagcagg cgatcgtgta
    12121 aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg ctatccagtg
    12181 gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca ccagcaacgg
    12241 cgcggttttc cagttccaga atgtcagact gaatattatg cagcaggtta tctggcgtca
    12301 catcgacaaa agcgtccagc tcctggctgc tctccaggtc agaaaggaga taaatgtagt
    12361 cgcgcccaag cttgccccat gaagccagca gcgggttgcc gacatcctgt tcaccatcgc
    12421 tgttaaagag ctgcccggca ttttcgctgt cgcgaaatag cggtaattcg cgatcttcaa
    12481 aactgtgtcg gcgctggcga gtcagtagtt tcgccagata agcgggatct ttaatgtcgc
    12541 cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt ttacccagcg
    12601 cctgtagcgc ctggagataa acaggcggta acgcggaaat accgcatata aagacgcgcg
    12661 aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag cgctgataga
    12721 gattggcgcg gtgccagcgc ggttgcccaa gttcgtcggt atattccacc aacgccttcc
    12781 acaacggcgc ttgccaggcc tgtgcttctc ccaacccttc taccagatgt cctgtttccc
    12841 actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc gccgcttttg
    12901 aagaaagctg gaacagtttt cgcttgtcac tatcgtcagt cagataatgc cgcaacaggg
    12961 taaagtcttc gcgctccaac agttgcggca gcagtgtcat cagtttccag ctcatgctct
    13021 gtttgttaaa ggcgctctct ttggggatct ccggtaacac ccggacgaac atatcccaga
    13081 taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt tgcgacaggg
    13141 tcatttgcag ccactgtgcc ataccggtac tttgcaccag aatcatctct ggttcgaaag
    13201 gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc acgtccagac
    13261 gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc gggcaatgca
    13321 ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg atgctgacac
    13381 atcccgcctg cgatgtctgc attcggttga cctgccagtt agcaggtggc gaaatcgcgc
    13441 gcagttgcgt ttgctgccag ccatgccgcc agagctgttg gtactggttt ctgctggcaa
    13501 gactgttcat taatgttcgc tggaaacccg ataacgcagt gacaatcatc accatcaaca
    13561 ccatcgccaa cattacctcc ggcagactaa agccttgttg attcctcagg gaagctgaca
    13621 taacgccctc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa aaacaatgtt
    13681 tccatcgatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccag caatcaataa
    13741 ggcttcatta tctgccagta aacgcagaca aacccgggca ccggtggcag catacagtaa
    13801 gcactgaact gcgggcagcg tctgccagga gtgcattttt ccccactcca gcgccgactg
    13861 aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagaag caaaactgcg
    13921 atcctgctga ctcattcctt gcaatagcaa gctacccaaa atcagcagca tcaggaccag
    13981 agccagtgac gaaacaccct tttcgcggtt cacaggttgg atcctgtcac gctatagctg
    14041 gcatccacca cggtttgcgg atcagccttg ctggcagcgt gcatattaac cgtcaacacc
    14101 ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat gatggcatcc
    14161 ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc
    14221 agcacatgct ccttcagacg aaatccaatc tggtcggact cttttaccgg ttcgcgatcc
    14281 cagataccat tactgttcgc atcccactgc acaatgacac agtcaccctg tccgacaatt
    14341 tccagccctt cgcccgtaca gctgccatgg caataacccg ccctctggag atgcttcgcg
    14401 acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt gttcgttaaa
    14461 ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac gctactgatc
    14521 gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg catccttctg
    14581 tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccactagc caccactcgc
    14641 ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg ccaaagaaag
    14701 caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgcggcaca aagaccaatg
    14761 gtgaactgcc atgacaggta ttcgccccag caacggaact cacaaggcac cataacgtcc
    14821 cctccctgat aagactgata ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat
    14881 agagcaaata gtcccgcgcc tggctggcgg tttgccaaag ccgttgcgac tgctgccagt
    14941 attgccagcc atagagccca cttgcgctta gcatgaccag aatcagcatc gcgaccagcg
    15001 tttcaatcag cgtataacca cgttgttttt tcatgccggc agtatggagc gaggagaaaa
    15061 aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta cgtcgttgca
    15121 ggacgttgca aaatttcggg aaggtgtctc gaagaattta acggagggca aaaaaaaccg
    15181 acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac cggcgcttta
    15241 atgcccggat gtggatcgta tccttcaatc tcaaagtctt cgaaacggta gtcgaagatg
    15301 gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg gcttaattgc
    15361 agatgagttt gatccatatg gttgctgtac agatgcgtgt cgccaccggt ccagacaaaa
    15421 tcacccactt ccagatcgca ctgctgcgcc atcatatgca ccagtaacgc gtagctggca
    15481 atgttgaacg gcaggccgag gaagacgtca caggagcgct gataaagctg gcaagagagt
    15541 ttgccgtctg ccacatagaa ctggaagaat gcatggcacg gtgccagcgc cattttatcc
    15601 agttcgccta cgttccacgc tgaaacaata atgcgacggg aatccgggtc gtttttcagc
    15661 tggttcagta ccgtagtgat ctggtcaata tgacgaccat ccggcgttgg ccaggcgcgc
    15721 cactgtttac catacactgg cccgaggtcg ccgttttcat cggcccattc gtcccagatg
    15781 gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc cctgcaggaa ccacagcagt
    15841 tcgtggatga tggaacgcag gtggcaacgt ttagttgtca ccagcgggaa tccatcctgc
    15901 aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt acggtcgttt
    15961 ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt catggttcct
    16021 caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat cacacccgcg
    16081 acaatcatcg ggatggaaag aatttgcccc atgctgatgt actgcaccca ggcaccagta
    16141 aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgctcc gtaaccaatc
    16201 aggaacaagc ctgagacagc tcccattggg cgcggtttac gaatatacag gttgaggata
    16261 ataaacagca ccacaccttc cagcagcagc tcgtaaagct gtgatgggtg gcgcggcagc
    16321 acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg tttgcagcag caaaatatct
    16381 tctgtacggg agccagggaa cagcatggca aacgggaagt tcgggtcaac gcggccccac
    16441 aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg aatgagtgga
    16501 gcaataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa gataatcatc
    16561 accacgataa cgccaatcag gccgccgtgg aaggacatgc cgccgtccca gacacggaaa
    16621 agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata accaatacgt
    16681 ccaccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc atttttggtc
    16741 cagccactgc ccggacgatt cgcccgtcgt gttgccagcc acattgcaaa aatgaaaccc
    16801 accagataca tcagtccgta ccagtgaagc gccacgggtc ctattgagaa aatgaccgga
    16861 tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt cttgttattt
    16921 cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc caaaggtgcg
    16981 aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata tgtatatgat
    17041 ccgcgctata accccccacg aatcagcccg cccatgccgc gacgctccat aaaggctgca
    17101 acctgatggc gaacttcggt cgccagttgc gcttccagac tacgctgcgc aagattttct
    17161 gcttcggcaa aatcaatgcg ccgcagcagg tattttacgc gcgctacaga acgtccgttc
    17221 atagacagat ggcgataccc aagcccaatg aggattgcca cgcacatcgg atcgcccgcc
    17281 atttcaccgc acaaacggag atcgattcca tgtatttccg cttcccgggc gatcatcgcc
    17341 agagctcgta acattgcagg atgaagactg tcataaatgt tcgccacccg ggtattgttg
    17401 cgatcaacgg ccaggatgta ttgcgtcaga tcgttggtgc caacagagat gaaatcgacc
    17461 cgctttgcca gatgcggcag cataaacacc attgacggca cttccagcat gatgccgata
    17521 cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc acgttcaatc
    17581 aggcggcgtg cttcgtcaac ttcatcgagg cttgtgacca tcggcaacag aatattcagg
    17641 ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa gatctccggc
    17701 tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc gctgatcggc
    17761 atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac gggtttatca
    17821 ttaaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga cggaaaacca
    17881 ctttgcagca tgaatgggat ttcagtgcga taaagtccta tgccatcaat acggctgccc
    17941 agtttttctt catgttccgg gcttaaaccg gcattgagca tgactttaat gcgttcgccg
    18001 ctttttaact gggcgggtaa attgacgtca tcttccgcca gacggctaag ctcgatctct
    18061 tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac cagcaattca
    18121 ccgcgataac catcgacgat cagcgtccga cgatgcagta ccgaaggctg aatatccgcg
    18181 cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga gttggcagcg
    18241 ccatcgcgca cgacaacacc gactaagcga tcctggggca gctcagcaag cgttgtcgct
    18301 gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc ttgattagcg
    18361 tcatcaagat gaaacagcaa tcgctgacca agtgcacgta aatcgccagc ccgctctttg
    18421 agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac cgtttttacc
    18481 gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg cagccgggta
    18541 tccgaaagca ggtgtgagta aagatcgaaa atagccgccg tttctttttg tgcaccggca
    18601 gcaaagcgtt tgctatagcg gcgaaactcg ttagccgcct cttccaacgc cccggtcagt
    18661 cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc cattaaaggt
    18721 aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg taatgcacgg
    18781 attcgcgtct ggcgatattg cccaaacaag gcagtcaact gcgactgaga aagaatagct
    18841 gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata ctggcgcaac
    18901 tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat tggtacgcct
    18961 aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct ggggtgcttt
    19021 tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac gatcccttca
    19081 tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gtccccgggt cgccatcagg
    19141 tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt atccatcgca
    19201 agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc gctggctacc
    19261 ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc tcttttacgt
    19321 cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat caccacactc
    19381 gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac ctgtctgact
    19441 ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct ggtttgcata
    19501 ttgatttctg catcgccgct caccagctgc aagagaaacc atttttgttt ttggccgata
    19561 caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg caaccagtta
    19621 cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc aaacaattca
    19681 cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg aaattgccag
    19741 gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca aatcacgata
    19801 cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa taaagcttta
    19861 tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc agggcattac
    19921 acactaataa caattgaata actcacagtt atgtgcagag ttataaacag aggaaggggt
    19981 ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta cggaattact
    20041 acgggaaaac ccggagcatt ctgaataaga cagatataga gattttaaaa tattcattat
    20101 cagtcaatgt attacatgtg atttgagtaa taaaaaagag taattcgtga cccaggtcac
    20161 acctctcatt tacgggttgg cgaaagatca accaacgccg tatttatcca cagaatgtgc
    20221 cactaagtta agcactgaac cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa
    20281 tggaaacagt agtgaaggtt tttcacagtt atcccagctt tctgtggata acatggtgta
    20341 agatcctgtt tattttcagt gaccagattt ggaaaacccg ttgcagtgtt gcgcaactcg
    20401 attaccggca acctaaaaag caatataaat cagagaattg aacaacgcat gtggaaaaag
    20461 ttacactgcg aatattcggc acataattgc tgtttgtttt ttaaccaagg tatcatgaca
    20521 tgtcccaacc tcgcccactg ctctctcctc ccgaaactga agaacaattg ttagcgcaag
    20581 cacaacaact ttctggttat acattgggag aactggcggc acttgccggg ctggttacgc
    20641 cggagaattt aaaacgcgat aagggctgga ttggcgtgtt actggagatc tggctaggtg
    20701 ccagcgcagg gagtaaacct gagcaagatt ttgctgctct gggcgtggaa cttaaaacta
    20761 tccctgtgga tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt gccccgttaa
    20821 cgggcaatag cggggtgaca tgggaaacca gccacgtgcg ccacaagctc aaacgcgtgc
    20881 tgtggatacc ggttgaaggc gagcgcagca tcccgctggc aaagcgtcgc gtaggatcac
    20941 cgttgctgtg gagcccgaat gaagaggaag accggcagct acgcgaagac tgggaagaat
    21001 taatggatat gattgttctc ggtcagattg agcggatcac cgctcgtcac ggggaatatt
    21061 tacagatacg accgaaagca gcgaatgcga aagcgctgac cgaagctatt ggtgcccggg
    21121 gcgaacggat tctgacgctg ccgcgcggat tttatttgaa gaagaatttc accagtgcgc
    21181 tactggcccg tcattttctg atccagtagc catcgctttg acctgccgct ttccgggcat
    21241 ataattaccg cttcattttt ttggcagggc tttttagatg ttatttgcat ggataaccga
    21301 tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg gagatcgttc ttgggatcga
    21361 caatattatt ttcctttctc tggtggtggc aaagctccca acagcacaac gtgctcatgc
    21421 gcgccgtctg gggttggcgg gagccatggt catgcgtctg gcgctgctgg catccatcgc
    21481 ctgggttacg cgcctgacga atccgctttt tacaatattc agtcaggaaa tttccgcccg
    21541 tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg aaagccagca aggaaatcca
    21601 cgaatccatc gaaggtgaag aagaagggct gaaaacacgc gtttcatcat tcctcggcgc
    21661 tatcgtgcag attatgctgc tggatattat cttcagcctc gactcggtga ttaccgctgt
    21721 gggtctgtca gatcacctgt ttattatgat ggctgccgtg gtgattgccg taggcgtgat
    21781 gatgttcgcc gcgcgctcga ttggtgattt tgtcgagcgc catccttcgg taaaaatgct
    21841 ggcgctctct ttcctgattc tggtgggctt taccctgatt ctggaaagtt tcgacatcca
    21901 cgtaccgaaa ggttacatct acttcgcgat gttcttctct attgcggttg aaagcctaaa
    21961 cctgattcgc aacaaaaaga atccgctctg atactccgta cgctctcctg cgggagggcg
    22021 tactcactcc tgttaccctc acagtttaag attttcctgc tttcaagata tataacgtcg
    22081 gtttataaac agactattat cataggtggg ccaaatgagg ataaccggat gaaaaaatgg
    22141 gcagtaataa tttctgcagt cggactggcg tttgctgttt ccgggtgttc cagtgattac
    22201 gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga aattgatgat
    22261 gataccgggc tggtgagtta tcacgatcag caaggtaacg cgatgcaaat taaccgtgat
    22321 gatgtttcgc aaattattga acgttaacaa ataaggtcag catccggctg gccttaagat
    22381 ttttctcttt cccttttccc ttccctctgc catttttata ttccttatgt cgtgattata
    22441 aaaaggaaac ggctatgcaa tatcaccgta taccccacag ttcgctggaa gtcagcacgc
    22501 tggggcttgg cacgatgacg tttggtgaac agaacagcga agccgacgcc cacgcacaac
    22561 tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg tacccagtac
    22621 ctccgcgccc cgaaactcaa gggctaaccg aaacctacgt cggcaactgg ctggcgaaac
    22681 atggcagccg cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg cgcaataatg
    22741 acaaaggcat ccgcccggat caggcgctgg atcggaagaa tatccgtgaa gcgctgcatg
    22801 acagcctcaa gcgtctgcag actgattacc tcgatctcta tcaggtgcac tggccgcagc
    22861 gcccaaccaa ctgcttcggc aaactcggtt atagctggac agattctgcg cctgcggttt
    22921 cgctgctgga tacgctggac gcactggcag agtaccaacg cgcgggaaaa attcgttata
    22981 taggcgtgtc gaacgaaact gcatttggcg taatgcgcta cctgcatctg gcggacaaac
    23041 acgatctgcc gcgtattgtc accattcaga acccctacag cctgttaaac cgcagttttg
    23101 aagtaggtct ggcagaagtc agccagtatg aaggggtcga actgctggcc tattcgtgcc
    23161 tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc aaaacccgct ggcgcacgta
    23221 atacgctctt tagtcgcttc acccgctata gcggtgagca aacgcaaaaa gccgtcgcgg
    23281 cgtatgttga tatcgccaga cgtcatagcc tggaccctgc tcagatggcg ctcgcatttg
    23341 tacgccgtca accgtttgtt gccagcactc tgctgggcgc aaccacgatg gagcagctga
    23401 aaactaacgt cgaaagtttg catctggagt tgagcgaaga cgtattagct gaaattgaag
    23461 cggtgcatca ggtttatacg tatcctgcac cataagatta acgccggtta aaaccggcgt
    23521 taaatattaa tgacggcgct gccagatcca cagcgccgtt attgccagcg caaacagcgc
    23581 accgaagcca atgccaatgg gcacgaccgg gatgcctacc attaccgcca gcgagtaaat
    23641 gcccagcatc aacaacatgg cgctgttttc gccaaggttt tgtactgcaa tcgcattccc
    23701 cgccccgacg ctttttttac cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa
    23761 accccccagc acgccaatca gcatcaacaa agcataggct ggcagcagct cgtgttgcag
    23821 ggagaaaatc agtaccacca cgccaatcaa aatcccggct ggcatacagc gtgacacggt
    23881 ttccagcgta actaacttcg ctgccgcacc tgcaccaacc acgataccaa tcgctaccat
    23941 cgcgttgaga taggtcggcg tagcgttatc ggtaataccc agcgccaccg gtacccacag
    24001 caccaacagg aaacgcagcg tgacacccgc tccccagaat aaactggtgc ccaccagcga
    24061 aaaacgcgtt tcaccattgc gccatagcga ggtgcaggca ttcaggaaac tgcgggtcat
    24121 gctgatgaga ttccaggact gccccggacg cgccgccgcc agtttgggaa tgtagatatt
    24181 ggcaatgacc gcgccaccgt aggccagtgc gcatgcggcc agggcgatca ggacatgcca
    24241 gtcagccaga acaccaccag ctacggaacc gagcaaaatc gccgctatgg tagacgcctc
    24301 cattaaaccg ttagctttta ctaacttact acccgtggtt aattcgccga gaataccgta
    24361 tttcgccggt gaataggctg cagcaccaac acccaccaac gtatagccga gaaacggatt
    24421 gataccaaag cagatactgg ctgcgcccag tagcttcagg ccgttggcaa acatcatcac
    24481 ccggcctttg gcgaagctat ccgccacctg cccgacaaac ggcgcaaaaa gaatgtaagc
    24541 acctacaaac accatttgca ggatgggctg gctccactcc ggatagaact gcgctttcag
    24601 taacgccaga gtagcaaaca gtagcgcatt atcgccaaac gcagagagaa actgcgccac
    24661 gataaccgct ttcatcccct tcgaccacaa cgaagtgtta gtgtgcactg actcactcat
    24721 cgtgttgttc cgcttcgtct acccagcttt tcaacgtgac aaagtcaggt ttgccgctgc
    24781 caagtaatgg catctgtttc agatagcgaa tatcgcgcgg tacagcaagc tccggcacgc
    24841 cgtgctcgcg ggcatactgt tgtaacttat cgcgcgtcag ttcgttatct gtggtgaaaa
    24901 gcaccagtgc ctcgcctttg ctggcatcgc tcttaatcgc agtggcatgg actttatctg
    24961 gcgaaacacc aagtgccagt tgttccacca tttccagcga caccatttcg cctgcaattt
    25021 tggcaaagcg ttttgcgcgg ccctgaatct gcacaaagcc ctgctcatca aaacgcacga
    25081 tatcgccagt gtcataccag ccgcgctcca tttcgccgcg aatattctcg gcggtgggca
    25141 cttccagtac accaggcttc tccacccgca gatagccgtt cattatgttc ggccctttca
    25201 gttgcaggcg tccgccctct tcgataccag gaaccgacaa caggcgcgca tccattcctg
    25261 gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg tacgttgata gaaacgacag
    25321 gcgcgcattc ggtcacaccg tagccttcaa ggatgcgcag gccaaattta tcctgccaaa
    25381 gctgtttggt actttcttgt agtttttctg cgcctgccac cacatagcgt agacgataga
    25441 agtcatacgg gttggcaaag cgcgcgtagt gaccgaggaa agtcgaggta ccgaacaaca
    25501 cggtgcaact gcggtcatac accaactccg gcacaatgcg gtaatgcagc gggcttggat
    25561 aaaggaacac ttctgcacct gtgagcagtg gcgtaaacag gcctaccgtc agcccaaagg
    25621 agtgaaacag cggtaacgcc gacataaagc gatcgttggt ggtgaagtcg gcaatcgttt
    25681 taatctgctc aacgttcgcc agaatgcttt tatggctatg gacgacgcct ttcggatggc
    25741 cttcagaacc ggaggtaaag aggatcagcg cttcttcttc cggctgctgt tttaactgtg
    25801 ccagacgcgg catcagcaaa tgagcgaaaa tccacacttt gtcggcagtg gtgacatctg
    25861 cttttaaatc ttccagatag acccagcgca cctgagtaag ttgctccggc agatgccaga
    25921 gtttgccttt atcgagaaac tgacgggaag tgaagatggt tttgatttca gccgccgtaa
    25981 tagcactggt cagccctttt accccggcgg tgtagttcat cattgcggga atgcggcgac
    26041 gggcgatggc cccaaaaatc actgccgcac tgatgcctgc attgggcagc attaagccga
    26101 tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc aacaaacagc gttttcgtaa
    26161 gcaatttgcg ataggagtct ggggtaaagt tgacgtcttc gacacatttc ttcccggctc
    26221 cgaagcggta cattgcgctc agtaaagatt cgtacagcgt ttcacgcggg cgcaccgcca
    26281 tacgcgcctc catcattatt tgatgcagca tttcgccagc gattttgcga cggtcacggg
    26341 cacgcggcgc atccggcatc tccacctgcg ttggtggcaa aatatgcaga gtaatttgcg
    26401 ggaacaagcg acgtttaacc agacctttca ggcggctgaa gtgcgtaagt tccgcccctt
    26461 caatacgcac aggaataacc gttgcaccag acttcgccgc gacaaatccc gcgccatcgt
    26521 agattttcat cagcgagcca gtggtggtga tgcgtccttc agggaaaatc accactggtc
    26581 ggccctgttc caccagacgt accagatgtt taatcgccat aggttgcgtc gggtcgagag
    26641 gaacaaagtc gataaatgat ttcagccaac gcatatacca ctgttggctt attgaggtgt
    26701 aaacggcaaa taccggacgt acgggtaaaa acaatgccag cagtatgcca tcaataaaag
    26761 agacgtgatt aggcgtgatt agaacgcgct cgcccttcag tgccttggtg tcacccgtaa
    26821 cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa gctaaaaagc attccaactc
    26881 cctttgctct gattcagtaa aagcgaatgg agggagatta cacgagataa agaacgcgag
    26941 cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc gcaggttggt gcaagagaca
    27001 gggtacgaag agcgtaccga ataatctcac caatcaatac ctctgggatc ttgattgtgg
    27061 tctgcacgac gactcttcgc cagcgagaaa acgcaaagga atgaagggaa atgcaacgag
    27121 gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg caaaaaaaaa cctgcgcatc
    27181 tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg acttcaccta tcaatacctc
    27241 tgggatcacc actttagcaa cctgaagcca aacgccacca gcggtcaatc gcaacagcgt
    27301 ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg tcttacttat ttgcgtttgg
    27361 ctcacattcc cacaatgaaa acacgccgcc ccttgaacca acgggcgttt tccgtaacac
    27421 tgaaagaatg taagcgttta cccactaagg tattttcatg gcgaccataa aggatgtagc
    27481 ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc attaataatt cacccaaagc
    27541 cagcgaagct tcccgactgg ctgtgcatag tgcaatggag tctcttagct atcacccgaa
    27601 cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg atcggtctgg tcgttggtga
    27661 tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac
    27721 tggtaatttt ttattgattg gcaacggtta ccacaacgaa caaaaagagc gtcaggccat
    27781 tgagcaactg atccgccatc gctgtgctgc gctggttgtc catgccaaaa tgatcccgga
    27841 tgccgattta gcctcattaa tgaaacaaat gcccggtatg gtgctgatca accgtatcct
    27901 gcctggcttt gaaaaccgtt gtattgctct ggacgatcgt tacggtgcct ggctggcaac
    27961 gcgtcattta attcagcaag gtcatacccg cattggttat ctgtgctcta accactctat
    28021 ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc cttgctgaaa gtggtattcc
    28081 ggccaatgac cggctggtga catttggcga accagacgaa agcggcggcg aacaggcaat
    28141 gaccgagctt ttgggacgag gcagaaattt cactgcggta gcctgttata acgattcaat
    28201 ggcggcgggc gcgatgggcg tgctcaatga taatggtatt gatgtaccgg gtgagatttc
    28261 gttaattggc tttgatgatg tgctggtgtc acgctatgtg cgtccgcgcc tgaccaccgt
    28321 gcgttaccca atcgtgacga tggcgacgca ggctgccgaa ctggctttgg cgctggcgga
    28381 taatcgccct ctcccggaaa tcactaatgt ctttagtccg acgctggtac gtcgccattc
    28441 agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc agcgactaac cgcagttaaa
    28501 gcaattccag cgccagtaat tcttcaatgg tctggcgacg gcgaatcaac cgcgcctgac
    28561 cattatcaaa cagaacttct ggtaacagcg gacggctgtt gtagttggat gacattgacg
    28621 cgccatatgc ccctgtatca tgcagtacca gataatcacc cgccttcact tccggcaagg
    28681 cgcgggtttc gacatttccc ccttcctgct gggtaaagac atcgcccgat tcacataacg
    28741 gcccggcgac gacggtttcc accgttggcg cgtgttccag agaacgacca tcagctgcca
    28801 gggcactgat atggtggtaa ctaccgtaca ttgccgggcg catcagatcg ttgaacccgg
    28861 catcaaccag aacaaagtgg cggctaccca tttgtttgac gctccgcact tgagtaatta
    28921 atacgccagc ctgcgctacc agaaagcgac ccggttcaat ttccagtttc acaggatggc
    28981 ccaaatggcg ggcgatttgc tcacgcgcgg cattccacag accataataa tgttcggtat
    29041 caaccgcctc ttcaccctgt tgataaggaa tagaaagccc accgcccgca gaaatagcct
    29101 gtaaatcctg accgaattcg aggacttgac gcaccatagc accacatacc tgttccagat
    29161 gggcataatc gacgccagaa ccaatgtgca tgtgaatgcc gaccagctgt agatgatgac
    29221 gttgtatcac gtccagtgcg gcgggcagat cggtgtacca gataccgtgc ttgctgtttt
    29281 cgccaccggt attggttttt tggctatgcc cgtgaccaaa ccccggatta acacgcagcc
    29341 atacccgatg ccctggcgaa acctgcccca gttggtcgag catatcaaca gaacccgcat
    29401 tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg
    29461 taaaaacaat atcatcaggg tgcgtttgcg gattgtaacc cgccgccagt gcacgctcta
    29521 tttcacctaa cgagacggaa tccactttta cgccctgctc acgcattaag cgcaaaatat
    29581 gaatattgga acaggctttc tgtgcaaagc gcaccacatc aaactgtttc agcgccgcaa
    29641 tctgccgacg aataatttgc gcatcgtaga cccacaccgg gcagccaaat tctgcgggca
    29701 aacgcagcag attttcggcg gtgagatcgg tatcagtgct gaacagtgaa tgtggcataa
    29761 caaactccag ataagtgctt ttttatgatt acgccacatc ataaaaagaa taaaaaatat
    29821 cgatttatgt cgagtctatg caaaaatgat atggattacc ggattgcgag agagcgctaa
    29881 tggccgccgt taacttacgt catattgaaa tttttcatgc ggtaatgacc gccggaagcc
    29941 tgactgaggc ggcacacctg ctacacacct cacagccaac cgtcagccgc gaactggcgc
    30001 gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcat acgtggacga ttacatccta
    30061 ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc ctggtacgga ctggatcgca
    30121 ttgtcagtgc cgcagaaagt ctgcgcgagt ttcgccaggg agaactgtct attgcctgcc
    30181 tgccggtctt ttcgcaatct tttttaccgc agctcctgca accctttctg gcacgttatc
    30241 ccgatgtcag cttaaatatc gtgccccagg aatcaccgct acttgaagag tggctctcgg
    30301 cccagcgtca tgatttagga ctcactgaaa cgctccatac gcctgcggga acagaacgta
    30361 ccgaattact ctctttagat gaagtgtgtg tgttacctcc gggccatccg ctggcggtaa
    30421 aaaaggtatt aacgccggat gattttcacg gtgagaacta catcagcctt tcccgtactg
    30481 acagctatcg ccagttgctg gatcaattgt ttactgagaa tcaggttaaa cgacgcatga
    30541 tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt acgggcgggg gtaggcgttt
    30601 cagtggttaa cccgctcacc gcactggatt atgcggcaag cggtttagtg gtgcggcggt
    30661 tcagcattgc ggttccattc accgtcagcc tgatccgccc cctgcaccgc ccgtcatcag
    30721 cgctggtgca ggcgtttagt gagcatttac aagcgggatt accgaaactg gtcacttctc
    30781 ttgacgctat tttgtcgtca gctacgacag cataaaagcg acagcatcct cggcatggat
    30841 cgccgcggta tcaaacacag gcagaacact gcgctcttct ggcaccagta aaccaatttc
    30901 tgtgcagcca aaaatgacgc cctgtgcgcc ctgttctgca aggcgagcaa tcacttgcgc
    30961 ataataagcg cgtgacgctt cggtaaattg ccccagacac agttcttcaa aaataatctg
    31021 attaattttc gcccggtcat ccgcttcagg aataagacag ttgatggaaa attgttccgt
    31081 cagccgcccg cgataaaaat cctgttccat ggtgtaacgt gtacccagca gcgccacacg
    31141 agtcattcct gccccggtaa ttgcacgtcc agtggcatcc gctatgtgta agaaaggcag
    31201 agagcaacga gactcaatgg catccgccac tttgtgcatc gtattggtgc ataacacaat
    31261 accttctgcg cccgcccgct gtaagccaag cgccgcctca gccagaatat ccccggtttt
    31321 gtcccactca ccgcgacgct ggcactcttc tatttcatga aaatcgacgc tatgtagcag
    31381 cacttgcgca gagtgaagcc caccaagccg ctgtttaatg ccttcattta tcagacgata
    31441 gtaaggaata gtggattccc agctcattcc tcccagcaaa ccaattgttt tcattcctct
    31501 ctcctgatga gtgttaccgc aacaaggctg aataaggaac gaggcaaacg cctcaacggc
    31561 caggtgccta atctctatta acgaaaaaag ggccggatgt acagcacatc cggcccgtga
    31621 aatcagacgc cgatatttct caacttttcg cctgccatca gtttgcgctc gatatgttcc
    31681 agcgtgacat ttttggtttc cggaatgagc cagaaagtga tgcccacaaa cgcaatgttc
    31741 agcgcagtgt agagccagaa cgtaccggca gcgccaatgc tatcaagcag tgtcaggaag
    31801 gtcgcgccga taatcatatt cgacacccag tttgtcgtcg ttgagcaggt gatgccaaaa
    31861 tcgcggcatt tcagcggctg aatttcagag cacaagatcc acaccactgg cgcggcgctc
    31921 atcgcataac cggcaataca catcatcgtc atgccaacag agagccagga caagccactg
    31981 gaagccgtac cgttatcaaa ctgcatcagg cagtaaccca gcaccagagt gcctaacgcc
    32041 atcacgctga aaccaatttt cagcgccggc ttacgccctg ctttatctac cgtaaagacc
    32101 gcaatgaagg tagcgaacat aaaggtcagc cccacgacca gagtcgcaat catctgttgt
    32161 tctgtggtcg taaagcccgc cattttgaag atacgtggcg cgtagtacat gatgatgttc
    32221 ataccggtaa actgctgcat cgcctgcaac aacataccga gaaatacagc acgacggacg
    32281 ttacggttga tcttaaacag tgcccaaccg ccctgtttta acttcaggct ttcgcgaatt
    32341 tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca gcatacgcaa tacttcttcc
    32401 gcctcaatat gacgcccctt ttccgccagc cagcgcgggc tatttggcag gaagaccacc
    32461 agaataatca gcagaactgc tggtaaagca agaaccccca acattgcgcg ccagttaccg
    32521 ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagagt gaccatcaac
    32581 tggtacatgc tgatcatctt accgcgaacg ttttcacttg ccatttcaga aagatataga
    32641 ggagcggtgt aagacgcgat cccgacagca atgcccaaca ccacacgagc ggcgattaac
    32701 atctctacgc tggtcgcaaa agcggaccct atcgaaccga gtacaaacag gatggccccc
    32761 gccatcaggc tgtatttacg ccccaggcgg aacgacagcc aaccattaaa cagcgcacca
    32821 attgctgcac cgagcatcat gctactgacc acccattcct gcaaacgact ggtcagcaca
    32881 aagtgatcgg taatgaacgg caacgctccg gcgattacgc cgatatcaag accaaataac
    32941 aatcctgcga ccgcagcagc taccgaaaca aacatattca tacgccgcgt atcccgcaga
    33001 ggacgtggcg ttaaagcaga ttccgtattg atagtaacca ttttttcctg ccagcagaga
    33061 gtaagacata gtgaaaaaat acgtgaacaa ttcacacagg tgtcaggtcg gaaatagcat
    33121 aaatatggat taaattgcta cg
//
LOCUS       DAPQZD010000043        32438 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPQZD010000043 DAPQZD010000000
VERSION     DAPQZD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.043"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            163..270
                     /locus_tag="RO454_001916"
     CDS             163..270
                     /locus_tag="RO454_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDY0933756.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(356..2035)
                     /gene="bcsG"
                     /locus_tag="RO454_001917"
     CDS             complement(356..2035)
                     /gene="bcsG"
                     /locus_tag="RO454_001917"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDY0933757.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPHYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPIVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2032..2223)
                     /gene="bcsF"
                     /locus_tag="RO454_001918"
     CDS             complement(2032..2223)
                     /gene="bcsF"
                     /locus_tag="RO454_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDY0933758.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2220..3791)
                     /gene="bcsE"
                     /locus_tag="RO454_001919"
     CDS             complement(2220..3791)
                     /gene="bcsE"
                     /locus_tag="RO454_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDY0933759.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4064..4252
                     /locus_tag="RO454_001920"
     CDS             4064..4252
                     /locus_tag="RO454_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDY0933760.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4264..5016
                     /gene="bcsQ"
                     /locus_tag="RO454_001921"
     CDS             4264..5016
                     /gene="bcsQ"
                     /locus_tag="RO454_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDY0933761.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5013..7631
                     /gene="bcsA"
                     /locus_tag="RO454_001922"
     CDS             5013..7631
                     /gene="bcsA"
                     /locus_tag="RO454_001922"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDY0933762.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7642..9981
                     /gene="bcsB"
                     /locus_tag="RO454_001923"
     CDS             7642..9981
                     /gene="bcsB"
                     /locus_tag="RO454_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDY0933763.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9988..11094
                     /gene="bcsZ"
                     /locus_tag="RO454_001924"
     CDS             9988..11094
                     /gene="bcsZ"
                     /locus_tag="RO454_001924"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDY0933764.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11076..14549
                     /gene="bcsC"
                     /locus_tag="RO454_001925"
     CDS             11076..14549
                     /gene="bcsC"
                     /locus_tag="RO454_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDY0933765.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14631..16619
                     /gene="hmsP"
                     /locus_tag="RO454_001926"
     CDS             14631..16619
                     /gene="hmsP"
                     /locus_tag="RO454_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDY0933766.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16802..18088
                     /gene="dctA"
                     /locus_tag="RO454_001927"
     CDS             16802..18088
                     /gene="dctA"
                     /locus_tag="RO454_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDY0933767.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18308..19804
                     /locus_tag="RO454_001928"
     CDS             18308..19804
                     /locus_tag="RO454_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDY0933768.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19900..20829)
                     /gene="kdgK"
                     /locus_tag="RO454_001929"
     CDS             complement(19900..20829)
                     /gene="kdgK"
                     /locus_tag="RO454_001929"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDY0933769.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            21061..21828
                     /gene="pdeH"
                     /locus_tag="RO454_001930"
     CDS             21061..21828
                     /gene="pdeH"
                     /locus_tag="RO454_001930"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDY0933770.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21898..23958
                     /locus_tag="RO454_001931"
     CDS             21898..23958
                     /locus_tag="RO454_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDY0933771.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(24137..25459)
                     /locus_tag="RO454_001932"
     CDS             complement(24137..25459)
                     /locus_tag="RO454_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDY0933772.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25852..26865)
                     /gene="yhjD"
                     /locus_tag="RO454_001933"
     CDS             complement(25852..26865)
                     /gene="yhjD"
                     /locus_tag="RO454_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDY0933773.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26913..27812)
                     /locus_tag="RO454_001934"
     CDS             complement(26913..27812)
                     /locus_tag="RO454_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0933774.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGS
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            28332..28934
                     /locus_tag="RO454_001935"
     CDS             28332..28934
                     /locus_tag="RO454_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDY0933775.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28985..30634)
                     /locus_tag="RO454_001936"
     CDS             complement(28985..30634)
                     /locus_tag="RO454_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDY0933776.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            31039..32436
                     /gene="ccp"
                     /locus_tag="RO454_001937"
     CDS             31039..32436
                     /gene="ccp"
                     /locus_tag="RO454_001937"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDY0933777.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
BASE COUNT         7773 a         8129 c         8880 g         7656 t
ORIGIN      
        1 cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg
       61 tacgttgcag gtatgcacct gacaacatca cgggggctaa tcttgaacct taacaactca
      121 agaatagccg cgaaacgttg tcattacaac acaggcggct atatgacgct cgcagagctg
      181 ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg
      241 atcgtgaact ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat
      301 ggcaatttgc gcccggacca ggccgcaggg ggaaactctg cggccttttt cgttcttact
      361 gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatcc tggtattgaa
      421 taactactgc atttgagttc tcggagaccg gtgctgtttg tggtaaccca ctggtgagtt
      481 ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga accaccagat
      541 cggagatagc caggaagctg ctcggttggt cgatgacaat cggagccccc tgatgtggtg
      601 ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg ctagggatat
      661 cacgtagacc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt tccggcacca
      721 cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg tccagttcat
      781 caaagaattt ttgcgcccgc gctttgtaat ccgctgtttt gctgacgccc ggataatggt
      841 tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt ttatcttttt
      901 cggtaacgtc cagccaacgg ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa
      961 agcccagcaa aataaccggc agatttgttt gatccatcaa ttcgctctgc atgccgccat
     1021 tttcgcgaac ttccttcaaa aaaccaccga actggccgtt atgtcccatc atcaggtgct
     1081 gggtaaagcc cagtttcgac aggttatcaa acagatagca gtcgttattt gccggttgat
     1141 acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc gccgccgggc
     1201 cactgtagga ggtggcggag ttaaagttct tgaactcaat atcgaaatgc gaccacagtg
     1261 gatgcgacat caatccggcg gcttcaatat ccgaccagga aagcgaacag atattaatca
     1321 ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaagatc gatttacgtt
     1381 tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gtttgctgtt gttggcggtg
     1441 cagtttgtgc gggcatatca cccactatcg gtgcgccacc cgtcgccgca acggttgccg
     1501 ctgcgttacc acccgtcgtt gttacagtgg tcgttggttg accggctggc cacaaggaga
     1561 aacttggtcc cgccagggta agtacgttca gccatagcag aatggcaacc acaaaaacgg
     1621 taatgcgaat ccattgtgac aggaataacc aggccacgaa taaaacaaaa atggccccaa
     1681 tcatctgcca gttaataaag cgtgtgacaa gatcgattaa ataatcggta ctgaaccccg
     1741 ccacctgcga accctggctc attatgcttt ccggaccagg caaccaggtg tcatgccaga
     1801 acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg ctatagtgcg
     1861 gaatgggcat cagtagaaac gcggcaaaca ccaaattgag gagcggatgg aagttaagat
     1921 atcccgccca caacaggccg aacttaacca gaaaatagaa gttccagccg gaaaggccgc
     1981 gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga gtcatttttt
     2041 ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat aacgaggttt
     2101 agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc
     2161 cagcgggaaa aatatcagtg cgcaaacgac aataatttca atgatatcgc tgatggtcat
     2221 catgatgagc gctccacagc atcatctaac agtcgcattg gttctggtat tcgtcgccag
     2281 tggcgaccat catgctcggc attgatgacc ggtttagaac tttgcgctaa aggcagcggc
     2341 atgccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact gatttgatca
     2401 tcttcaaacc agaccattcg gtttgagaaa atgtcgccag taggcaatgg gaaaatatga
     2461 ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac cagccgatta
     2521 ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag ggcttgctca
     2581 acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg ggcaggtaat
     2641 agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt ctggaaacca
     2701 cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg cacatagcga
     2761 ctaaacttct gcccttgcac gctttcgatc atcgtcagac agcgggagag tggcgcattc
     2821 cacgggataa ccatatttgc accgcaggcc aataataaac gttcatcggt ggcgcgcagg
     2881 ctggcggtat tttcccgtac gaggattttc atcgcactac cgcgctggcg acgcagagta
     2941 tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc
     3001 gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa cagttgccag
     3061 tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat gcgtttttcg
     3121 tcgctgcgtg gttggatttc cgcctcttgg tgttgaacta atgtccagca accattttgt
     3181 tgctgaacgc taagttgctg acgggcgctg accccttttt cgttgcacca gaaagcaata
     3241 tccagcaaat gttggtcgcc ctgaaaacgc aaactggcaa gaccaaaaag tgaccgatat
     3301 tcctcaagta acaatgaaaa ttgtttatcg ttattatttc cggaattaat taccaaaagc
     3361 gaacaatggt ttagccggct ccatttattc attttatcca accatgagcg aagccgctcg
     3421 gcaggaatgt tttgccatgc attatttgcg caaacaagaa taaaaaggta attatgggga
     3481 tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg atttagcatc
     3541 gaaaataatg ttattttttc cggcccttga gaatcatcta attgaaagat tttcgctgga
     3601 tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc aattgtttga
     3661 ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac gccgcctgct
     3721 ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac agggtccaca
     3781 atgtccctca tgtcaacatt ttgtctgatt atcagtttac tagcgaaagc taagaaataa
     3841 acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta gcaggtcaca
     3901 agacatgtga ttttagtctt ttcgttttta actttttctg ttgcataacg gaaagtcaaa
     3961 aagtgagcac attcccgtct cgccgtaaac aataaaccga cgaaatgctc acagttagaa
     4021 ttgtctcatg aacggtacgg ttatttcata gggatcaaga aaaatgaata acaatgaacc
     4081 agatactctg cctgatcccg cgataggcta tatcttccag aatgatattt tggcgttaaa
     4141 gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg aacagttggc
     4201 cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt aggggattgg
     4261 tgaatggccg tactgggatt gcagggggtt cggggaggcg tggggacaac aaccatcacc
     4321 gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt cgatgccagc
     4381 ccggacaact tgttgcgcct gtcatttaac gttgatttta cccaccgtca gggttgggcc
     4441 agagctatgc tggatggcca ggactggcgt gacgccgggt tgcgctatac ctcgcagctc
     4501 gatttgctgc cttttggtca gttatcgatt gaagaacaag aaaatccaca gcactggcaa
     4561 acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg gcgttaccag
     4621 tggattttaa ttgacttacc gcgtgatgcc tcgcagataa cccaccagct gctgagtttg
     4681 tgcgatcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg actgcatcag
     4741 caagcgctgc cggatggtgc acatattttg attaatgact tccgtattgg cagccaggtt
     4801 caggacgata tttaccagct ttggttgcaa agccagcgcc gattgctgcc gatgctcatt
     4861 catcgtgatg aagcgatggc tgaatgtctg gcagctaagc aaccagtcgg tgaatatcgc
     4921 agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct gttgaattat
     4981 tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg tggttgctta
     5041 tcccgccggt caacgcacgg cttattgggc gttatcgcga ttatcgtcgt cacggcgcgt
     5101 cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt tttattccgc
     5161 tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat ccgcatatca
     5221 acgcctcgcg tccgcgtccg ctggacccgg tccgttatct tattcaaaca tgctggttac
     5281 tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag gcgggcattt tcaggtctac
     5341 aaaatattcg tggacgttac catcaatgga tgaatgagct gcctgagcgc gttagccata
     5401 aaacacagca tcttgatgag aaaaaagagc tcggtcattt gagtgccggg acgcggcggt
     5461 tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc gttactcagc
     5521 cgtttaaccc gctagcgcag tttatcttcc tgatgctgct gtggggggta gcgctgatcg
     5581 tacggcggat gccggggcgc ttctcggcgc tgatgctgat tgtgctgtcg ctgaccgttt
     5641 cttgccgtta tatctggtgg cgttacacct cgacgttaaa ctgggacgat ccggtcagcc
     5701 tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg ctggtgctcg
     5761 gctatttcca ggtcgtatgg ccgctgaatc gtcagccggt gccattgccg aaagatatgt
     5821 cgctgtggcc gtcggtggat atctttgtcc cgacttacaa cgaagatctc aacgtggtga
     5881 aaaataccat ttacgcttcg ctgggtatcg actggccgaa agacaagctg aacatctgga
     5941 tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg ggggtgaaat
     6001 atatcgcccg taccactcat gaacatgcga aagcgggcaa catcaacaat gcgctgaaat
     6061 atgccaaagg cgagttcgtg tcgatattcg actgcgacca cgtaccaacg cgatcgttcc
     6121 tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg cagacgccgc
     6181 accacttctt ctcgccggat ccgtttgaac gcaacctggg gcgttttcgt aaaacgccga
     6241 acgaaggcac gctgttttat ggtctggtgc aggacggcaa tgatatgtgg gatgccactt
     6301 tcttctgcgg ttcatgtgcg gtgattcgcc gtaagccgct ggatgagatt ggcggtattg
     6361 ctgtcgaaac ggtaaccgaa gatgcgcaca cttctctgcg tctgcaccgt cgtggctaca
     6421 cctcagcgta tatgcgtatt ccgcaggcag cggggctggc gaccgaaagt ctctcggcgc
     6481 atatcggtca gcgtattcgc tgggcgcgcg ggatggtgca aatcttccgt ctcgataacc
     6541 cgctcaccgg taaagggctg aagtttgctc agcggctgtg ctatgtcaac gccatgttcc
     6601 acttcttgtc gggcattcca cgactgatct tcctgactgc gccgctggcg ttcctgctgt
     6661 ttcatgccta catcatctac gcgccagcgt tgatgatcgc cctgttcgtg ctaccgcata
     6721 tgatccatgc cagcctgact aactcgaaga tccagggcaa atatcgccac tctttctgga
     6781 gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg gtggcgctga
     6841 ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg gaagaagagt
     6901 acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctgctcaac ctggtgggcg
     6961 tagcggttgg gatctggcgc tacatctatg gtccgccaac cgagatgctc accgtggtcg
     7021 tcagtatggt gtgggtattc tacaacctga ttattctcgg cggcgcagtc gcagtatcgg
     7081 tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc gcagcaattg
     7141 cccgcgaaga tggtcacctc ttctcgtgta ccgttcagga tttctccgac ggtggtttgg
     7201 ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat ctgttgctta
     7261 aacgcggtca gcaggaatac gtcttcccgg ctcaggtggc gcgcgtgatg ggtaatgaag
     7321 ttgggctgaa attaatgccg ctcactaccc agcaacatat cgattttgtg cagtgtacgt
     7381 ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat aagccgctgg
     7441 aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg gcggaatttg
     7501 cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc tgggttgtat
     7561 cgtttattcc gcgccgcccg gagcggagcg aaacggcaca accatcggat caggctttgg
     7621 ctcaacaatg atgataacgc gatgaaaaga aaaatattct ggatttgtgc agtggctctg
     7681 gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca accactgatc
     7741 aatgctgagc cagctgtagc cgcccaggcg gagcaaaatc cgcaggtggg gcaagtgatg
     7801 ccgggcgtgc agggcgcaga tgcgccagtc gtggcgcaga acggtccttc ccgtgatgtg
     7861 aagctgacct ttgcgcaaat tgcgccgccg ccgggcagca tggtgctacg tggcattaac
     7921 ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc
     7981 aacctcgaat acacgccatc gccatcatta ctgcctgtcc agtcgcagtt aaaggtttat
     8041 ctcaatgatg aactgatggg cgtgctgcca gtgaccaaag aacagttggg taaaaaaacg
     8101 ctggcacaaa tgcccattaa cccgctgttt attactgatt tcaaccgtgt gcggctggag
     8161 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gcaccacgct ttggctggat
     8221 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
     8281 tcacacttcc cggtgccatt ctttgacccg cgtgataacc gcaccaacac cttgccgatg
     8341 gtctttgcgg gtgcgccgga tgttgagctg caacaagcat ctgccattgt cgcctcgtgg
     8401 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaactgccg
     8461 gatcgcaatg ccattgtctt tgccaccaat gacaaacgcc cggacttcct gcgcgatcat
     8521 ccggcggtaa aagccccggt gattgagatg attaaccatc cgcagaatcc ttacgtcaaa
     8581 ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct
     8641 cagggtaaca ttctgttccg tggtgaaagt gtggtagtga atgaagtgaa accgctgcta
     8701 ccgcgtaaac cgtacgatgc gccgaactgg gtgcgtaccg atcgtcctgt cacctttggt
     8761 gaactgaaaa cctatgaaga acaattacaa tccagcggtc ttgagccagc agcgattaac
     8821 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
     8881 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
     8941 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgcctgctg
     9001 ctgcgtattc cggtattaca aggtttgctg gatggcaaaa cggatgtctc tattccggcg
     9061 ctgaaactgg gcgcgaccaa ccagttgcgg ttcgactttg agtatatgaa cccgatgccg
     9121 ggcggttcgg tggataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
     9181 gacgactcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
     9241 gcctttgcta acgcgggctt cccgttcagc cggatggcgg atttgtcgca aaccatcacc
     9301 gtgatgccga aaacgccaaa cgaagcacag atggaaacct tgctgaatac cgttggcttt
     9361 atcggtgcgc agacgggctt cccggcgatt aacctgacgg tgaccgatga tggcagcacc
     9421 attcagggca aagatgccga catcatgatc gtcggtggta tcccggacaa actgaaagac
     9481 gataagcaga tcgacctgtt ggtgcaggcg accgaaagct gggtgaaaac gccgatgcgc
     9541 cagaccccgt tccccggtat tgtgccggac gagagcgatc gcgcggcaga aacccagtca
     9601 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
     9661 cagcgcagcg tgattgcgct gctggcagat agcccacgcg gttatgaaat gcttaacgat
     9721 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
     9781 tccggtatca acagcctacg tgttggcgat gtttattacg taggtcatct gccgtggttc
     9841 gagcgcttgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
     9901 agtgtgatat tgctggcgtg ggtactgtgg cgtctgctgc gtattattag tcgtcgtcgt
     9961 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggact cgtgacgatg
    10021 ctgctgctgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg ggagcagttt
    10081 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    10141 accacctccg aagggcaaag ttacggcatg ttctttgccc tggcggctaa cgaccgtgca
    10201 gctttcgata atcttctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    10261 aatttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    10321 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    10381 tggaaagagc agcgttatac cgacatcggc agcgcattgc taaaacgtat cgcgcgggag
    10441 gaagtggtga cggtgcctgg gctgggctcc atgttgttac cgggcaaagt gggttttgct
    10501 gaggataaca gctggcgttt taaccccagc tacctgccgc cgacgctggc gcagtatttc
    10561 acccgctttg gcgcgccgtg gaccacgctg cgcgaaacca atcaacgttt attgctggaa
    10621 accgccccga aaggcttttc gccagactgg gtgcgctatg agaaagacaa aggctggcag
    10681 ctaaaagccg aaaaaacgtt gatcagcagc tacgacgcta tccgcgttta catgtgggta
    10741 ggcatgatgc ctgacagcga tccgcaaaaa gcgcggatgc tcaaccggtt taaaccgatg
    10801 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    10861 gcgcagggta aaggaccggt cggtttttct gccgccatgc tgcccttttt acaaaaccgc
    10921 gatgcgcagg ccgttcagcg ccagcgcgtg gccgataact ttcccggcag cgatgcctat
    10981 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt ccgcttctcg
    11041 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    11101 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    11161 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagac ctggtgcaac
    11221 agtcgttgta tcggctggaa cttattgatc cgaataaccc ggatgtgatt gctgcccgtt
    11281 ttcgttctct gttacgtcag ggcgatattg atggcgcaca aaaacagctc gatcggctgt
    11341 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctgctttcca
    11401 cgccggatgg tcgtcaggca ctgcaacagg caagattgca ggcaactacc ggtcatgcgg
    11461 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    11521 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    11581 agctaaagcg cattaatgcg gatacgccgg gcaacacggg cctgcaaaac aatctggcgc
    11641 tattgctgtt tagtagcgat cgccgtgacg aaggctttgc cgtcctggaa caaatggcga
    11701 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    11761 ccgtcagcga tgccagcgtg caggcactga aaaaatatct ctcgatcttt agcgatggcg
    11821 atagcgtggc tgctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    11881 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggcatggcg ggtaaagcca
    11941 ttcccgaact acaacaggcg gtgcgggcga acccgaaaga cagtgaggct ctgggggcgc
    12001 tgggccaggc gtattcccag aaaggtgatc gcgccaatgc agtggcgaat ctggaaaaag
    12061 ccctcgcgct ggatccgcac aacagcaata acgacaaatg gaacagtctg ctgaaagtaa
    12121 accgctactg gctggcgatc cagcagggcg atgccgcgct gaaagccaat aatcctgacc
    12181 gggcagaacg gctgttccag caggcacgta atgtcgataa caccgacagc tatgcagtgc
    12241 ttgggctggg cgatgtggcg atggcgcgca aagattatcc cgccgccgaa cgctattacc
    12301 agcagacctt gcgtatggac agcggcaaca ctaacgccgt gcgcgggctg gcgaatattt
    12361 accgccagca gtcgccagaa aaagccgaag cgtatatcgc atcgctctct gccagccagc
    12421 ggcgcagcat tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag
    12481 aggcactgga aaaccagggg aaatgggcgc aggcggcagc acttcagcgg caacgactgg
    12541 cgctggaccc cggcagtgta tggattactt accgactttc gcaggatctc tggcaagccg
    12601 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag ccgaacgacc
    12661 cggagcaggt ttacgcttac gggctgtatc tctccggtca cgaccaggac agagcggcgc
    12721 tggcgcatat caacagcctg ccgcgcgcgc agtggaacag caatattcag gagctggtta
    12781 atcgactgca aaacgatcag gtgctggaaa ccgctaatcg cctgcgagaa aacggtaaag
    12841 aggcagaagc ggaagcgctg ctgcgccagc aaccgccttc ctcgcgtatt gacctcacgc
    12901 tggctgactg ggcgcagcag cgacgtgatt acaccgccgc ccgcgcggct tatcagaacg
    12961 ttttgacgcg cgagcccact aacgccgatg ccattcttgg tctgacggaa gtggatattg
    13021 ctgccggtga caaagcggcg gcacgtagcc agctggcgaa actgcctgcc actgataacg
    13081 cctcgctgaa cacacagcgg cgcgtggcgc tggcacaggc gcagcttggc gataccgcag
    13141 cggcgcagca gacatttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    13201 aaagcgcgat ggtactgcgt gatggtgcga agtttgaagc gcaggcgggc gatccaatgc
    13261 aggcgctgga aacctacaaa gacgccatgg ttgcatccgg tgtgaccacg acgcgtccgc
    13321 aggataacga cacctttacc cggctgaccc gtaacgacga gaaagatgac tggctgaaac
    13381 gcggcgtgcg cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcactctcg
    13441 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacca
    13501 ctatgttgca ggtggacgcg ccctattctg acgggcggat gttctttcgc agtgatttcg
    13561 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    13621 gcacctgtac attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg
    13681 tggcggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    13741 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    13801 acaccgttaa cgcccaccgt cggcctatct ccagttcttt gctggccttt ggtgggcaaa
    13861 aagactctcc gagcaatacc gggaaaaaat ggggcggcgt gcgtgccgac ggcgtggggc
    13921 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agcggtgatc
    13981 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    14041 ataaggtcat caaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    14101 attacgacaa agacctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    14161 aatacctgtc gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    14221 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    14281 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    14341 gcagcagtca gggctttggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    14401 actggtttgt cggcacggca attgatatcc agcaggcaaa agattacgca cccagccatt
    14461 tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccaccgc
    14521 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    14581 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    14641 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    14701 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    14761 cgcaactgga aagcattgct cgctctgtcc gcgaaccctt atcttctgcc attttgaaag
    14821 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    14881 gtgccgatgt agtgctgccc aaccagttcc aggcgctgcg taaaagtttt attccagagc
    14941 gtccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggtgcaaatc tcgctgggcg
    15001 tttactcgct ggaacgtccg gcaaacccgc agccaattgc ctatctggtg ctacaggcgg
    15061 attccttccg catgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact
    15121 tacttttgtc gttaatattg actgtggcga ttagctggtg cattaaccgc ctgattttgc
    15181 atccattacg caatattgct cgcgaactta acgccatccc accccaggat cttgttggtc
    15241 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt
    15301 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    15361 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggtgg
    15421 tggcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    15481 cgggtgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    15541 aatcggtact gtcgccacgc atgatcctcg cgcagattag cggttatgac tttgctgtca
    15601 ttgccaacgg tgtacaggaa ccgtggcacg ctatcacatt aggtcagcaa gtgctcacta
    15661 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    15721 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    15781 catttaccgc tcgccataaa ggcaaaaatc agattcagtt ctttgatccg cagcagatgg
    15841 aagccgccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    15901 agtttgcgat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    15961 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    16021 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    16081 gtcgattgct tgctgcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    16141 cagcgctgca actgatgcat ccaaatatgg tggcggatat gctggaactg ttaacccgct
    16201 atcgcattca gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgatc
    16261 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    16321 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    16381 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggttt gccggaagat agcagcatga
    16441 ttgctgcaat tatcatgctg gcgcagagcc tgaacctaca aatgattgcc gaaggcgtgg
    16501 agactgaagc acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    16561 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaaatagc
    16621 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    16681 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    16741 ttttaaggcc gcaggtaccc cataacctta caagacctgt ggttttacta aaggacaccc
    16801 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    16861 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    16921 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttctgtaccg tcgtaacggg
    16981 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    17041 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    17101 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    17161 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag
    17221 cgtcattggc gcatttgcta gcggtaacat tctgcaggta ctgctgtttg ccgtactgtt
    17281 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    17341 tttctcgcag gtcattttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    17401 gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    17461 acagctgatt atctgtttct acatcacctg tatcctgttt gtggtgctgg tattgggttc
    17521 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgttacatcc gtgaagaact
    17581 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    17641 ggagaaactc ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt
    17701 taatcttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    17761 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    17821 taaaggggcg gcaggggtaa cgggtagtgg ttttatcgtg ctggcagcga cgctctctgc
    17881 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    17941 agaagctcgt gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    18001 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    18061 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    18121 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    18181 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    18241 taacgtcatt tagactgacc gacaacgggc agttttttat taccaggata gttgatcagg
    18301 ggttcacatg cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac
    18361 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    18421 ttccaacggt ttacagtggc aagtgctgac tacaccccag cgtcccagcg atcgtgttga
    18481 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    18541 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cgcaggcgcg
    18601 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    18661 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgacttgc tgaaagaggc
    18721 gctctcttat ctggcaaatg ccactggcaa actgactatc acgccagaaa ccatcaacca
    18781 tgcgttgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    18841 gcgttatcgt ctgaaaggat caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    18901 acccgttgaa gcggaaaaga ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    18961 gacgctgctg gtggtgggaa acgtggatgc gcgctcggtc gtcgaccaaa tcaataaaac
    19021 gtttggcgaa ctgaaaggca aacgtgaaac accggctccg gtgccgacgc tttctccgct
    19081 gcgtgcggaa gcggtgagta ttatgaccga cgcggtgcgt caggaccggt tatctatcat
    19141 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    19201 ggacctggcc cgtgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag
    19261 taaagacatc ggtcttggat ttgactgtcg tgtgctgtat ctgcgtgcgc agtgtgccat
    19321 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    19381 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    19441 gaaactggag ctgcagaagc tgtttgccgc ctatgcgcga gctgataccg atattctgat
    19501 ggggcagcgg atgcgttcgt tgcaaaatca ggttgtcgat atcgcgccgg agcaatatca
    19561 gaaactgcgt caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg
    19621 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    19681 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgcggctgc
    19741 cgccacatct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    19801 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    19861 gggggtaaca ctctatccag ccttttgcat atgctgcttt tacgctggca tcgcttcacg
    19921 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    19981 tgcgtcttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcac tgaaagagtc
    20041 gccagctgcg gtggtatcga tcactttttc tttcggcagt tttaccgccg gaacatccac
    20101 taacccttca ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    20161 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    20221 cgcgtcttca tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta
    20281 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    20341 cacttttccg ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    20401 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    20461 ttcgcaaatc gtcgcggatt gctcactctc cagccagaat ttggcggcgg cttcgttacg
    20521 ccagtagtag aacgtacgtt cgccggtgct gtcggtttca atgtagtaaa ggcccggcag
    20581 acggttttcc atccgttggg tcagggaagt atcaacgttc tcgccgcgcc aggcgtccag
    20641 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    20701 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgcccc cgaaaccgcg
    20761 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    20821 tttggacata gttgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    20881 ttgctggtca accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    20941 gaaagattcc gttgtggagt ggggaaattt accccggcca ggcggtaaag ttctgccttt
    21001 gcgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    21061 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    21121 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    21181 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    21241 caacgcctgc cgccggatcg ctattttact gaaatcaccg ttagccatcg gatggaggtt
    21301 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttgtaga gcacggcctg
    21361 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    21421 ctgcgccaga ttgaggagct tccctggctg cgtttcgaac tggtggaaca catccgcctg
    21481 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt
    21541 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    21601 gcgcgagaac tgtttgtgat gctgcgtcag tcaccggaag gacgcacact cttttctcag
    21661 cttttacatc taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacacca
    21721 gaagagtggc gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    21781 cgcccggcac cgatagaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    21841 tccgcctggc tggactatct ttaggactgg cacagaaaga atatgaggaa gcgaacgatg
    21901 agcaaggcag gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    21961 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    22021 gtctctgcgg agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag
    22081 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggccccatgt acacgcggaa
    22141 gacatcattc ttggcaatcc accggatatt cccgaagtca caatggtgca tttgccgcga
    22201 gtagaagcga cgctggcccc gctggcgctg ctgaccaaaa cggtctggtt gccgtggatc
    22261 aagctcgaaa agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg
    22321 tttaatctcg ccaacgatga caacaaagac gcgaatgcaa agccgtcggc gtggtcgttt
    22381 cggctggata atattctgtt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    22441 gcggatctgg aaatttttgt cgatccgtta ggcaagccgc tgtcgttcag cgaagttacc
    22501 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg
    22561 cagggacgtt ataacggtga accgctcact ggtacgggaa aaataggcgg tatgctggcg
    22621 ctgcgtggcg aaggaacgcc gtttccggta caggctgatt tccgctctgg taacacccgt
    22681 gttgcttttg atggcgttgt gaatgaccca atgaagatgg ggggtgtcga tttacggctt
    22741 aaattttctg gcgattcgct gggtgatctc tatgaactga cgggcgttct gctgcccgat
    22801 acaccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgatactga aaaatcgtcg
    22861 gtctttgatt atcgcggttt taatgggcgc attggcgata gcgatatcca cggttctctg
    22921 atctacacca ccggcaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    22981 cggctggcgg acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg
    23041 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    23101 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg
    23161 cggcgcattg agcatggcag cagcctgccg attagcgatc tttctactca tatcatcctc
    23221 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tagcgggcgg cagcattgcg
    23281 gcgaatattc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    23341 cgtcgactga aactgaaaga actgatgccc gatgtagaac tgatgcagaa gacgctgggg
    23401 gaaatgaacg gtgacgcgga actgcgcggt agcggtaact cggtggcggc gcttttaggc
    23461 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    23521 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgatgatgag
    23581 gtgcgggtga actgcgcggc ggcgaatctg gatattgcca acggcgtggc gcgcccgcag
    23641 atttttgctt tcgatactga gaacgcgttg attaacgtta ccggtacggc aagttttgct
    23701 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacattg
    23761 cgttcgccgc tgtatgtacg tgggacgttt aaaaatccgc aggctggggt gaaagccggg
    23821 ccgttgattg ctcgcggagc cgttgcggcg gcactggcaa cgctggtaac accggcggcg
    23881 gcgttactgg cgctgatctc accttccgaa ggggaggcta atcagtgcag gacaattttg
    23941 tcgcagatga agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt
    24001 tagattgttg gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc ctccggctta
    24061 caaatccttg cgaattcaat atattgtatg gaaattctgg cctgataagc gtagcgcatc
    24121 aggcttttca ctcttattac aacgactgat gtcgcgtctc atgggtcagc agcagggcga
    24181 ttaacgtcaa gccagccatc gccgccagat atagccccac cgcacctaac ccgtagttag
    24241 tctgcaacca ggctgcgata tacggcgcaa cggaagcccc gagaatcgac gatacgttgt
    24301 aagagaacga tgctccggtg taacgcactt ctgtcgggaa cagctctggt aacagcgcgc
    24361 ccatcggacc gaaggtcagc cccatcagac ttaaccccag cagcagaaag gcaaaaacca
    24421 gaatcgggtt gccagaaccg agcaaagggg taaaggcgaa tagcgcgaac aggatgatca
    24481 gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt aatccggcga
    24541 ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac acttcgttac
    24601 gcggcaggcc aagcccaact ggcgcggcgg cggtactaaa ggtcatagag tagaccgtca
    24661 tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg gttacgcgaa
    24721 catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct ttagcgactt
    24781 tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata atgaccagca
    24841 ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctcg tcggtcagta
    24901 gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt gcgcccagct
    24961 gcgggaagga gccgtacaat gcacgtttgc gcggtggggc gttttcagtc gccagcagcg
    25021 ccgcgccgcc ccattcacca cctaagccca gaccctgacc aaatcgagcc agcgccagca
    25081 gcagcggggc gaaaatacca atcgtggcat agcctggcag cagaccaatc accacggtcg
    25141 aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa
    25201 aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg aaggtggcga
    25261 gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc ggaaacacaa
    25321 taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg ccaatgagag
    25381 aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgctcg tggtcgagtg
    25441 ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag ccatattacg
    25501 cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa aatcaggcaa
    25561 aaagggaata atgtttaggc cagtgatttt tcttctatga aatgcttcac ataattttat
    25621 aaatggtgga ttaaactggt tttaggttaa ataaaagtta ccaactggat ttatatgctg
    25681 gggttatgag ttggaatgaa attgtttgct cctgaataga tggctaaaga ggctctggga
    25741 aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg catcttatcc
    25801 ggcctacggg gagccaggcc ggataaattc gtacgccgca tccggcctat tttaaggctg
    25861 tgttttcccc ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca
    25921 aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca cggaaccaaa
    25981 tgctgcgcca gagggtgatt tcatcaacga tggcagggtg taggtcatca cgattttaat
    26041 cacttcaaaa ccaatagcag ccagaaatgt tccgcggatc agggcttttt tgcgcggacg
    26101 gtggcgcggc aaccgccaga agatccagaa gaaaagcaga tagttggcga agatggaaat
    26161 cgccacgcca atcaaccgcc atgtcggttt cagccactca atgctgttca ggtgcagggc
    26221 gctaataatc atttgctgcg cagaaccggc aaccgaggtg atagaaagcg tcacaatcag
    26281 cgcaatcaac aaaccaatca gcgaaataaa atcacgcaga tattttaccc agaacttctc
    26341 ctgatcttgc ggcgagcgtt cccagacatc gcgtgactgg gcgcgaatcg cttcacgcag
    26401 gttacccatc cagttgatgc cggaataaag tgccactgcc agaccgacaa gccctacagt
    26461 cgtacgctgc tgaacggcgg tgttgatggt gtttttcaat gtggcggcta gcgtcggatc
    26521 gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat gggaggcgag
    26581 cacaaagccc cctgcggcaa acgacaccat caaaatgggg atcatcgaca aaaatgagaa
    26641 ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg
    26701 aatcaggtgc gcaataaccg gttggcgttg gactttctcg gcagtggtgg tgacggtttc
    26761 cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa tcggctcgtg
    26821 ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct cttttgtcct
    26881 tctgtcgtgg tcattcttaa aagtatagtc ggtcagtcca ggtactcttt cattacgcca
    26941 gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg cggataaacc
    27001 agtgaaaggg acagcggctc ggcacggtag ccaggcaaca cttcaataag ccgcccggca
    27061 cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc ggcaagaccg
    27121 gcggcgagat agttttcgct actgtttacc gtcaacatgc tgccagactt aaaccactgg
    27181 acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg cggtgtataa
    27241 cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga
    27301 tagtgcggac tggcacagtt gaccatggtc agtttaccga ggggacgggc gataacgcca
    27361 tcttccggta atgcgccagt gcgtatcacg caatcaaaac catcatgaag aatatctacc
    27421 ggacggtcat ggctactcag ttccagctca attcccggat gcagatagag aaattccgac
    27481 aagcgcggca gtaacaggct tttcgcgatt cctgacggga tgtcgatgcg taatttaccg
    27541 ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt actcaacaca
    27601 tcttttgctc gttgataata ggtcatgcct tctggcgtga gtttgacccg tcgcgtggtg
    27661 cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg cgaaacactt
    27721 ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc cgcgactttg
    27781 atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg ttcacaacga
    27841 aacagtagcg tgcatatcgc attgtttatt gttttttcta acatctaatg ccgggtttca
    27901 caatgttgtc atgatgaatg tggcgatttc tcagcaggaa aagggaggaa tagctttatg
    27961 agaaaggtaa attcccttaa acagagcgta tttttcagcg attcccttta aagtgaatag
    28021 aagatgtatt ggctatttgt gcttattttt atctcattgc cgtttgttat ttttgtttat
    28081 ggtcgcggaa tttcattttt aattgcatgc gtactaacta ccgatataag gaaatcctca
    28141 aaaatgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt aactatttat
    28201 atcggtaact attttctacg tgtcatctct gtagaatggc caccgccaaa tggcgaattc
    28261 ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg tattcaggtg
    28321 gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt catggaatga
    28381 aaatcagttt acagcatcgt attccaggag tgagtattca gggggccagt caggcagacg
    28441 agttatggca aaagctggaa agttaccctg aagccttagt gatgctcgat ggcgatcagg
    28501 atggtgagtt ttgctactgg ttgctgcaaa aaactgtggt gcaatttcct gaggttaagg
    28561 tgttaattac ggcgacggat tgcaacaaac ggtggttaca ggaagttatt cattttaatg
    28621 tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg
    28681 cggcgatggg gatgatgttt ctgcctggag actggcgaac gacgccggaa aaggacatta
    28741 aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta gcggcgggcg
    28801 aatcaaataa agagatcggc agggcgctga atatcagtac cggaacggta aaagcgcatc
    28861 tggaatcact gtatcgccgt ctggaagtca aaaatcgcac ccaggcggca atgatgttaa
    28921 atatctcctc ctgaagagga taattttaca gccagcagga agttagcggc tggctgtaaa
    28981 aatattatgg ttcgccgtac aaaccaatta aacggcgtac cacaccgtta gtccagccaa
    29041 acccatcctg caacggatac tcgccaccgc cgccttcgcg aggaacacca tcggcaatat
    29101 ggtatttctc gatcagtttg tgctgttcca gatagaactg attcaccgtc ttcagccagt
    29161 tgcgcgcgat ttcatcaccc agaaggtcat cgccgtacat cttaaatccc tgaattgcca
    29221 tccactgtaa cggtgcccag ccgttgggtt tatcccactg ttcaccggtt tcgtactcgc
    29281 ttgccagaat cccgccaggt gtcagtaacc ggctgcgcac ggcgtttgcc agacgatcgg
    29341 cctgttcatg gttcgccata ccgacataga gcggcacaat ggcggcagcg gaaaacagcg
    29401 ccagttgctc gcgtcgccag tcgtaatcgc ggtagatacc gttttcatca tcccagaggt
    29461 aacggtttac cgcatcgcga cgggcactgg ccttctggcg gaacagtgct tctgtctctt
    29521 tctcgccttt cagtgccgag atgttggcga tggcgctctc cagtttaaac aggaaggcat
    29581 tcagatcgat ggggatgaac tgggtggtac gaatgctcgc cagacgacca gtatcacgca
    29641 gccaacggga agagtaatcc caaccggagg ccgcccccgc acgtaaatcg cggtacacct
    29701 cgttaggcgg gcgaccagaa tgtttcgcgg tttcaacgtc ctcaagccag gattcgtcac
    29761 gcggcgtgtc gcgatcgtcc cagtaacggt tgagcagcga gccgtccggc atccgcacaa
    29821 catggcgata ggcctgatta gggatcaacg attctgcacc gtccatccag aaggcatatt
    29881 ccattttcag gtggtcgaga tagcggcgcg cgccgcgtac accatcttct tcaaacaact
    29941 ccaccatcag cgcaaaaacc ggtggttgcg agcggctcaa ataataggtg cggttgccgt
    30001 tggggatgtg gccgtagttt tcgatcatcc aggcgaagtt atcggccatg catttcagca
    30061 aatcttcccg accactttcc gccagcccca gcatggtgaa ataggaatcc caatagtagg
    30121 tttcgctaaa acggccgccc gggacaatat atgactgcgg cagcgccagc agagaagacc
    30181 acggaatgtg atcctgtggt tcacgggtta gcaccggcca cagctggtcg atatgctctt
    30241 tcagggaatt ttgcgggtcc gatacatact cgctggagta gacctccggc agccagaagt
    30301 ggttttcaac aaacttgcgc aagtcaaaat cacgatgacg gcgcacttta cggtagcgga
    30361 ttaaaatatc cagcgggtcc attttgggtg cgcagtcggg aaaggtttta ctgtcgggga
    30421 aaattttcgc cgactgaaca tgctcgaaca gttcgagata gcgatcggca ggcgtcagcg
    30481 catcagaggc gggcagccct tcaatcatct cgggttccgg ttctgcctcg atcatctcat
    30541 ccagttttaa ttcatacggg tccagctcat agcagagatc gacctcgatc atcagttcgt
    30601 ctggattagg gttttgaatt ttctgattga gcataaggat ccgacctccg aaattcgtcg
    30661 ctaattatcg ggcttttctt taagggtagt cattcgctcc gcctggtggg cgacaaagcg
    30721 tgcggtggat cacgtttatt aacctgccaa aaaatataac gcactaatat caatggaatt
    30781 tataaaaata cggatttcgt gttgtttttc attttcagaa cattcaatcc tgctctcgcc
    30841 gcaatcatga tatgccattc ctgatctggg taataggcac tggctatctt attgatagtt
    30901 tatattcatg taattgattg attcctatca aaaagcatat ctccctgttt tttatgatgg
    30961 ctttgtctcg cagagacgtt tgcagcattc tggaaaattt tctgggtcac aataactcac
    31021 taccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg cctggctggc
    31081 gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa taaacgcagt
    31141 aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt aggctttctc
    31201 cgcgaaaaag gatgcgatta ttgccatacg ccttcggcag aattacccgc ctattattat
    31261 attcccggcg cgaaacagtt gatggattac gacataaagc ttggatataa atcttttaac
    31321 ctcgaggccg tgcgtgcggc tctgctggct aataaacccg tttcgcaaag cgatctgaat
    31381 aagattgaat gggtgatgca gtatgaaact atgccgccaa cgcgttatac cgcgctacac
    31441 tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat tgcaaaacag
    31501 cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga accggtgcag
    31561 cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggttt tgcgctgtat
    31621 cacgatcccc gtttatcggc ggatagcacc atttcatgcg ctcattgcca tgcgttgaat
    31681 gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt tgggccgatt
    31741 aacgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga tggtcgtgcg
    31801 gcaacattgc aggatcaggc tggtggaccg ccgttgaacc cgattgaaat ggcgtcgaaa
    31861 tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc gcagttcctc
    31921 gaagtctatc cgcaaggttt cagtggcgaa aatattactg atgccattgc tgaatttgag
    31981 aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga tgagaatgct
    32041 ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg tgcaacttgt
    32101 catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa aaaagacttt
    32161 aactttggtg aaattacggc ggcggatatt ggtcgtatga atgttactaa agaagagcgt
    32221 gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc accgtacttc
    32281 catcgcggtg acgtgccgac gctggacggg gcagtgaaac tgatgctgcg ctatcaggta
    32341 ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca cagtctgaac
    32401 ggggtttaca tgccgtatat gcaggataaa caataatt
//
LOCUS       DAPQZD010000044        31444 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPQZD010000044 DAPQZD010000000
VERSION     DAPQZD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.044"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(46..642)
                     /gene="nrfG"
                     /locus_tag="RO454_001938"
     CDS             complement(46..642)
                     /gene="nrfG"
                     /locus_tag="RO454_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDY0933778.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(639..1022)
                     /gene="nrfF"
                     /locus_tag="RO454_001939"
     CDS             complement(639..1022)
                     /gene="nrfF"
                     /locus_tag="RO454_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDY0933779.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1015..2733)
                     /locus_tag="RO454_001940"
     CDS             complement(1015..2733)
                     /locus_tag="RO454_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDY0933780.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2753..3709)
                     /gene="nrfD"
                     /locus_tag="RO454_001941"
     CDS             complement(2753..3709)
                     /gene="nrfD"
                     /locus_tag="RO454_001941"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDY0933781.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3706..4377)
                     /gene="nrfC"
                     /locus_tag="RO454_001942"
     CDS             complement(3706..4377)
                     /gene="nrfC"
                     /locus_tag="RO454_001942"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDY0933782.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4374..4940)
                     /gene="nrfB"
                     /locus_tag="RO454_001943"
     CDS             complement(4374..4940)
                     /gene="nrfB"
                     /locus_tag="RO454_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDY0933783.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4985..6421)
                     /gene="nrfA"
                     /locus_tag="RO454_001944"
     CDS             complement(4985..6421)
                     /gene="nrfA"
                     /locus_tag="RO454_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDY0933784.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6813..8771
                     /gene="acs"
                     /locus_tag="RO454_001945"
     CDS             6813..8771
                     /gene="acs"
                     /locus_tag="RO454_001945"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDY0933785.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8981..9295
                     /locus_tag="RO454_001946"
     CDS             8981..9295
                     /locus_tag="RO454_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDY0933786.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9292..10941
                     /gene="actP"
                     /locus_tag="RO454_001947"
     CDS             9292..10941
                     /gene="actP"
                     /locus_tag="RO454_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDY0933787.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(10980..11669)
                     /locus_tag="RO454_001948"
     CDS             complement(10980..11669)
                     /locus_tag="RO454_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDY0933788.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(11662..12072)
                     /locus_tag="RO454_001949"
     CDS             complement(11662..12072)
                     /locus_tag="RO454_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDY0933789.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            12176..13060
                     /locus_tag="RO454_001950"
     CDS             12176..13060
                     /locus_tag="RO454_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0933790.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(13096..14745)
                     /locus_tag="RO454_001951"
     CDS             complement(13096..14745)
                     /locus_tag="RO454_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDY0933791.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(14896..16245)
                     /gene="ghxP"
                     /locus_tag="RO454_001952"
                     /gene_synonym="yjcD"
     CDS             complement(14896..16245)
                     /gene="ghxP"
                     /locus_tag="RO454_001952"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDY0933792.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(16792..17256)
                     /gene="soxR"
                     /locus_tag="RO454_001953"
     CDS             complement(16792..17256)
                     /gene="soxR"
                     /locus_tag="RO454_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDY0933793.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            17342..17665
                     /gene="soxS"
                     /locus_tag="RO454_001954"
     CDS             17342..17665
                     /gene="soxS"
                     /locus_tag="RO454_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDY0933794.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(17668..19254)
                     /gene="pdeC"
                     /locus_tag="RO454_001955"
     CDS             complement(17668..19254)
                     /gene="pdeC"
                     /locus_tag="RO454_001955"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDY0933795.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLFLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            19683..19964
                     /locus_tag="RO454_001956"
     CDS             19683..19964
                     /locus_tag="RO454_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDY0933796.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(20063..20599)
                     /gene="ssb1"
                     /locus_tag="RO454_001957"
     CDS             complement(20063..20599)
                     /gene="ssb1"
                     /locus_tag="RO454_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDY0933797.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            20854..23676
                     /gene="uvrA"
                     /locus_tag="RO454_001958"
     CDS             20854..23676
                     /gene="uvrA"
                     /locus_tag="RO454_001958"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDY0933798.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPDGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(23711..24067)
                     /locus_tag="RO454_001959"
     CDS             complement(23711..24067)
                     /locus_tag="RO454_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDY0933799.1"
                     /translation="MTILELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(24071..24487)
                     /locus_tag="RO454_001960"
     CDS             complement(24071..24487)
                     /locus_tag="RO454_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDY0933800.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(24598..25311)
                     /gene="aphA"
                     /locus_tag="RO454_001961"
     CDS             complement(24598..25311)
                     /gene="aphA"
                     /locus_tag="RO454_001961"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDY0933801.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            25588..27408
                     /locus_tag="RO454_001962"
     CDS             25588..27408
                     /locus_tag="RO454_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDY0933802.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEKHE"
     gene            27401..28759
                     /locus_tag="RO454_001963"
     CDS             27401..28759
                     /locus_tag="RO454_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDY0933803.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(28748..29821)
                     /locus_tag="RO454_001964"
     CDS             complement(28748..29821)
                     /locus_tag="RO454_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HDY0933804.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     TKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(29833..31332)
                     /locus_tag="RO454_001965"
     CDS             complement(29833..31332)
                     /locus_tag="RO454_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDY0933805.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSSAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT         8038 a         8356 c         7838 g         7212 t
ORIGIN      
        1 ttatttttta tgcagctcaa taacataatc ttcatcacca gatgattaat ctgatctccg
       61 ctgcaacaat ttcgccatat taatcgactc gaccagctgt gtccggttga tccggggtga
      121 gttgagatcc atcacttttt gccatagttc gatggcttgc gcgtaattcg cctgcataaa
      181 cgcatcggaa gccagcagca tcagagcggt gatttcgtta gagtccagcg cgagggcttt
      241 gtcgatcatt gcgcgagcct ggacggtcat atgttggctg gcctggtaat acagcaccgt
      301 cgccagcgcc gcatacagtt cagcgttctc accacgtagt tgcaacgcct gacggtacgc
      361 cagcagcgaa ttgctgtaat cgttttgcca cagataatac tcgcccagta acgcccactg
      421 ttcgctgttt tgtggattag cacggatttt atcctgcaat gcctgaagct gcgcttccgg
      481 ggtttgctgg ctggcaaact gatgcagcgg atcgcgctga cgctgatact ccgcacgtac
      541 cgcctgccat tttggactta acagataact gccgacacag agaaatacca tcaaaatcgt
      601 gagtgttgtc agcattttca ccggtatttg gggctgtttc atcgcttcgc cctcactcgc
      661 cagaggatca gtgccatcag cagtaacaac actactggca gcgcccacaa caccagagtc
      721 tgacccgtta acggtgggtt atagcgaaca aaatctccgt agcgttcggt catccagcca
      781 atgatttcga cttcactttt cccctcttcc accatggtgt aaacctgatg gcgcatactg
      841 actgccaccg gcgcattgga ttccagtaag ttctgattct ggcactgcgg acaacgtaac
      901 tggctggcaa tatttaacgc ctgttcctgt tgttgcggat tagcgaattg ccaggtgtct
      961 acaacctgag cgcgcgcaaa acaggtaaat aacagcagta gcgtgagaag ccctttattc
     1021 atcgcgcttc cttccccgcc atccgcttaa caatgcgccc gcaatcatca acaatcctcc
     1081 cccccagatc cagcgcacac cgctttgtac atacaaacga aatgcgtaac gatccgctcc
     1141 ggttttctct cccatgaccg cataccagtc atggatgccg ttccagcgaa tcgagggttc
     1201 catcatttgc tgacggcgtg cttcgtaaaa acgccgctcc ggcgttaatt caccaatgcg
     1261 ttgctgatgg tcaaacagtg ccactatcgc tttttcgctg gtgtaattgc ctttggcttg
     1321 cagatcgagg cgctcaaaac ggaaggtgta tcctgccagc gtcacctgct gacccggctg
     1381 taaattcagg ctgatctcct gacggctgac gctggaaacc acgatccccg cggcaaataa
     1441 cagcacgccc gcatgcgcca gcagcgtcgg aagccgcgcg cgtttgccag agacaaacgt
     1501 cgccagcaca atcaccacca gcatcaacag agcaaacggt aacgtcgcgc ggttaaaata
     1561 cggcgcgcca acggagaggc gtccccagcc cagcaggccg taaatcatcg gataaagcgt
     1621 tcccaccagc acgatcagta gcactgcgca aaacagcaac agcgtagcga ggattaacat
     1681 ttcccgcgat aaccccgaaa aacgcaccgc cgggccaccg tcccgcgctc gccagccata
     1741 cagagccaga gacgcaaggc taatcagtac aaacaggccg aacaacggca cggcgcggac
     1801 gttatccagc gcgaacgcat gaaccgaaac cagaatgcca gaacggacaa tcaacgtgcc
     1861 cagcagcgac agcatcagag taactatcgt cagcaacagc gaccagtggc ggaaaatccc
     1921 ccgctggcgt gtcagggata aactgtgcag cagcgctgtg gcagaaagcc agggtagtaa
     1981 agaggcgttt tccaccggat cccagaacca ccagccgccc cagcccagtt cgcaataggc
     2041 ccaccaggaa ccgaggatga tccccgctgt taatgcgctc cagccaggta gtgcccaacg
     2101 ccagcaaatt cgggcgcagg taccatcaaa ctcgccgcgc agtaaactcg ccagcgccac
     2161 gctcgccgcc accatcaaac cgccgtagcc gagataaagc agcggtggat gaaagataag
     2221 accgggatgt tgcagcatcg gattgagatc gcggccttcg attgctggtg gaaatatccg
     2281 cacgaaggga tcggaccaca gcaccacaaa cagtagcagt gcggcgagca tgagagataa
     2341 aacggctagc gtcagcggaa atagcggatc ggtttgctgc cggtaatgcc aggcaaacag
     2401 cgcgctccag gcggaaagca gcagcaccca aagcaacagc gaaccttcat gaccgcccca
     2461 caccgccgcc agtttgagtt cccacgacag caggctatag ctatgttggg cgacataaat
     2521 aaccgagaaa tcgctgatga gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag
     2581 cgcgaactgc gccagaatgc cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg
     2641 cactcccgcg aaggccgtca acgggatcaa cacgttgacc ccgagactta acaacaacgc
     2701 cagaaaaccg acttcaggaa ggaatacgtc cagaaaccac ctctgttctg gcttacgcca
     2761 ccgttaactg cccggcgtag agaatgaaaa agcgcagcat caacacgccg accaggctgg
     2821 ccccacatgc cgccagcacg gcaggaatgc cggaactgcg attgacccac ggtttgagca
     2881 acattggcac aatcagcccc agcccggcga caccaagcca gaaccaccac gtccagaaac
     2941 cgccgcccaa tgccgccacc agcgcacgca ctttaccgtc atcgcccagc gccagaccga
     3001 caaaaaacgc caccagcagg aagatttcac cccataccac ggggatttcc atacggtgta
     3061 caaagtgcgc ttccgtggaa tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg
     3121 ccaccgccgc accggacgag atgccggaga agaggaacag caccggcagg atcgggttat
     3181 tgaggaacgg atacgatttc agcgccgaca gcagaaagcc ggtataagcc cccaacaaca
     3241 ccgccagcac cagcatcaat gtttccagtc cgcgatgaac gggcgtcagc aggctcaaga
     3301 ccttttgcac gatccccagc ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt
     3361 caaagatttt cgccagccac agcaccagca ccaccatgta aagctgaaac agcatcaccc
     3421 ccatcgacat caccgaggta aaactgtagt ggaacatcag cttccagaag gtccacggtc
     3481 ttgtcaggtg gaagacgagg atcaacagac cgaggatcac cgcgccaggc ccgacaatca
     3541 gcgtggtgcg cagcaacgta ctgtctgcac cgcctgcctg cgggtagaag cgacgtaaca
     3601 gcacggccag tgtcaccaga ccggcagaaa taccaatcaa aaacaggtag atggcaatcg
     3661 gccagtccca caccagcgat tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc
     3721 cccgtattta aacggtacgc ggtacagctt cggtttggtg cccagcgcca gcttgtagcg
     3781 gtaagtgggc ttctggcgca gcagttgcga aatctcactg ttgggatcgt ccagattgcc
     3841 aaacgtcagc gccttcgtcg ggcaagcttc aacgcacgcg ggcagcttac cggcctgcaa
     3901 atgggtctta cggcagaaat cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg
     3961 cacgcggtac ggacaggcgg cgatgcagta ctgacaaccg acgcagagat ccggattaac
     4021 atcgacaatc ccactggcag cgtcgcgaaa agacgcaccg gtcgggcaga cgtcaacgca
     4081 cggcgcatga tcgcagtgct ggcaagactt acggaagaag cgatatttca catcaggaaa
     4141 ttcgccctgc ggctcgctac gaataatcgt caggcgcgag acgccttccg gcaccttgtt
     4201 cactttccga caagcatcca tacaggcggt acagccgatg cataacgact cgtcatgcac
     4261 catgccgtaa cgcaccccat tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc
     4321 gctgacggcg gccagcacac cgacgccggt gagaaactgg cgacgagacc aggtcatggc
     4381 tgctccttaa gcaacggaac agacgccggg ttaaagttcg gattggtgcg ctgatcgctg
     4441 tggcaatcga cgcaaatctt aatccgtcct ttgtcactta acgtctgcat cgtatcttgc
     4501 tgtggatgca gggaatggca gctggcgcac gccactttgg ttacgtggac atcgtgcggc
     4561 cagaatgctt tttgcaactg ttccggcaga tggcaggaca tacagacgct gttctgctct
     4621 ccaaccttgt acatcggctc gttaaagcgc atcacatctt tcaccccttc gcggtgttgc
     4681 ggtgatggct ggccgtgaca gttggtgcag gtgaccggca gtttgttatt cgggttgata
     4741 acggaagcat gtttgccatg cataccttcg gtatctggct tatgacagtc cagacaggcg
     4801 gcatccgggt tacgctgctg ggtaacttcg tagcgatcac cagatgcctg cgccgcaggg
     4861 gtagcggtaa ttccgttcag gctccacaac aggcccgacg ccagcacccc ggcagttaat
     4921 aacgaacgta atacgctcat attcactcca ttgtgttttc cgccccttgc gaggcggaac
     4981 ggggttattg gcttaacaga ccgtttttac gtgcctgctc ttcccactgc gggatcaccg
     5041 ttttgatgaa gtcctgcttc tcggccttga tttgctccat gttcaggcca atggcctgct
     5101 gggctttctc tttggtcgag atatccggga tctggatttc atgggtaatg cctttggtcg
     5161 ccagcaggcg cgccagtttg gtgcgtgcat ccgccgcttt atccatcgcc gtaccgagca
     5221 tcctcagacc ttcttccggt gcgtgcatat gaatgccgtg ggaagcgatc gccaaatccc
     5281 aacgccactg ggcatgacgg atatcgtcct gaatcggctt catttcggct tccgttgcgc
     5341 ctgcatccag cgccgctttc gcttcgaagt gagcgtgaac cagttgatct tcaaccttga
     5401 ttttcaggtc gttaatcgac tgcttacgtt ccgcgaccac tttttgcaag gcagctttgt
     5461 cctgggtatg gcagttcgca caagtctggg cgaagttatc aaacggatta ccaattttat
     5521 ggtcggtgta gagtttgcct tcggcgttct gcacttttgg catatggcaa tcgatacagg
     5581 tcacgttgtt tttgccgtga atgcccgctg tccaggtttc atattccggg tgctgcgctt
     5641 tcagcattgg cgttttcgac agggagttag tccagtcaga gaaggcaatt ttgtcgtaat
     5701 actgctccat attttcgact ttcatgccgt catcccacgg gaatttaacc gctttgtttt
     5761 tgccgtcgaa gtaatactcc acatggcact gaccgcaaac catcgattgc tggtcgaaac
     5821 gtccggcttt ctcaaaaggt ttaccaatgg cttccatcgc gcgagccgca tacggacggg
     5881 aaagggttaa ctccggtttg cctttggcga actctggtga ggcggtgtta tggcaatcgg
     5941 cacaacctaa gttgttgacg atttccgggc cgccgcgcgc ccatttaccg tggaagtagc
     6001 catcttcgcc gtctttctgg atcagacgcg ccacatccgg gcttttacaa ctccagcacg
     6061 ccatcggtag cggaccatct tcagcgtttt tcggcgcacc ggtacgcaag gtttcacgca
     6121 catcggtcac agcaaaggca tgtccacgcg gcttgttgta atcgcgcgag aagggatacc
     6181 ccgcccacag gatcaccagc cgtggatctt ccgccagggc gtcaacacgc tctgactgtt
     6241 ccgaggtagc tttccaggag agatattgat cgggatgctg cggggcaaag gtttcattct
     6301 tcgcttccac agttacaggt gttgcgggag cagccgtttg ttcagcgtga acagaagtga
     6361 aaaagaaaaa aggaatcaat aagctgaaga tacggcgtgc gtttattttt atccttgtca
     6421 tagggcttca tccgaattga gccattgttg caatggcggt ttttattgtt tttcacgaca
     6481 gtaaccgcac ctacactgtc atgacattgc tcgcccctat gtgtaacaaa taaccacact
     6541 atgaatgttg tctttaatca attgtaagtg catgtaaaat accactttag agttagtcgg
     6601 tatcttcctc tttttcaaca acatgcataa ctgcatgttc ctcaaagaat taatcaactt
     6661 ttgttgctga ccttcaaaaa ttaccctgcc gtttatttgc acaattctac ttttgcgtga
     6721 tctgtcgccc aaatactaaa caaaactgcc aataccccta catttaacgc ttatgccaca
     6781 tattattaac atcctacaag gagaacaaaa gcatgagtca aattcacaaa cacaccattc
     6841 ctgccaacat cgcagaccgt tgcctgataa accctcagca gtacgaggcg atgtatcaac
     6901 aatctattga cgcaccagat accttctggg gcgaacaggg aaaaattctc gactggatca
     6961 caccgtacca gaaggtgaaa aacacctcct ttgcccccgg taatgtgtcc attaaatggt
     7021 acgaggacgg cacgctgaat ctggcggcaa actgccttga ccgccatctg caagaaaacg
     7081 gcgatcgtac cgccatcatc tgggaaggcg acgacgccag ccagagcaaa catattagct
     7141 ataaagagct gcaccgcgac gtctgccgct tcgccaatac cctgctcgaa ctgggcatta
     7201 aaaaaggtga cgtagttgcg atttatatgc cgatggtacc ggaagccgcg gtcgcgatgc
     7261 tggcctgcgc ccgcattggc gcggtacatt cggtgatttt cggcggcttc tcgccggaag
     7321 ccgttgccgg acgcattatc gactccagtt cacgactggt gatcacttcc gacgaaggtg
     7381 tgcgtgccgg gcgtagtatt ccgctgaaga aaaacgttga tgacgcgctg aaaaacccga
     7441 acgtcaccag cgtagagcat gtggtggtac tgaagcgtac tggcggaaaa attgactggc
     7501 aggaagggcg cgacctgtgg tggcacgacc tggttgagca agccagcgat cagcaccagg
     7561 cggaagagat gaacgccgaa gatccgctgt ttattctcta tacctccggt tctaccggta
     7621 agccaaaagg cgtgctgcac actaccggcg gttatctggt gtacgcggcg ctgaccttta
     7681 aatatgtctt tgattatcat ccgggcgaca tctactggtg caccgccgat gtgggctggg
     7741 ttaccggaca cagttatttg ctgtatggcc cgctggcctg cggcgcgacc acgctgatgt
     7801 ttgaaggcgt accgaactgg ccgacgcctg cccgtatggc gcaggtggtg gacaagcatc
     7861 aggtcaatat tctctatacc gcgcccacgg cgattcgcgc gctgatggcg gaaggcgata
     7921 aagcgatcga aggcaccgac cgttcgtcgc tgcgcattct cggttccgtg ggcgagccaa
     7981 ttaacccgga agcgtgggag tggtactgga agaagatcgg caacgagaaa tgcccggtgg
     8041 tcgacacctg gtggcagacc gaaactggcg gtttcatgat cacaccactg cctggcgcta
     8101 ccgagctgaa agccggttcg gcaacacgtc cgttcttcgg cgtgcaaccg gcgctggtcg
     8161 ataacgaagg taacccgctg aagggggcca ccgaaggcag tctggtgatc accgactcct
     8221 ggccgggtca ggcgcgtacg ctgtttggcg atcacgaacg ttttgagcag acctacttct
     8281 ccaccttcaa aaatatgtat ttcagcggcg acggcgcgcg tcgtgatgaa gatggctatt
     8341 actggatcac cgggcgcgtg gacgacgtgc tgaacgtctc cggtcaccgc ctggggacgg
     8401 cagagattga gtcggcgctg gtggcgcatc cgaaaattgc cgaagccgct gtggtcggta
     8461 ttccgcacaa tattaaaggt caggcgatct acgcctacgt cacgcttaat cacggggagg
     8521 aaccgtcacc agaactgtac gcagaagtcc gcaactgggt gcgtaaagag attggcccgc
     8581 tggcgacgcc agacgtgctg cactggaccg actccctgcc taaaacccgc tccggcaaaa
     8641 ttatgcgccg tattctgcgc aaaattgcgg cgggcgatac cagcaacctg ggcgatacct
     8701 cgacgcttgc cgatcctggc gtagtcgaga agctgcttga agagaagcag gccatcgcga
     8761 tgccatcgta acccacaatt gtcggatgcg ctccgaccta catctggcaa cggctgtagg
     8821 tcggataaga tgcgccagca tcgcatccga caacaagcgc cggatgcggc gtgaatgcct
     8881 tatccggcct acattcggca agggttaccc aagcgttaac cttctcccat aagggagcgg
     8941 gaattaaaac aatccctaca ttacctctgg agaatctgtg atgaatggta cgatttatca
     9001 gcggatagaa gacaatgcgc atttcaggga gttagtcgaa aaacggcaac ggtttgccac
     9061 catcctgtcg attattatgc tggcagttta tatcggcttt attttactga tcgccttcgc
     9121 gcccggctgg ctgggcaccc cgctgaatcc gaacaccagc gtcacacgcg gtattccagt
     9181 cggtgtcggg gtaattgtga tctcctttgt tctcactggt atctacatct ggcgggcgaa
     9241 cggcgaattc gaccgtctta ataatgaagt cctgcatgag gtacaagcat catgaaaaga
     9301 gttctgacgg cgcttgccgc cacactccct ttcgcagcta acgccgcgga tgctattagc
     9361 ggggccgtag agcgccagcc aacgaactgg caggcgatta ttatgttcct gattttcgtc
     9421 gtgtttacgc tcggcattac ctactgggcg tcaaaacgcg tacgttcgcg taatgactac
     9481 tacaccgcag gcggcaatat caccggtttt cagaacgggc tggcgattgc cggggactat
     9541 atgtccgccg cctcattctt ggggatctcc gcgctggtgt ttacctccgg ctatgacggc
     9601 ttaatttact cgctgggctt cctggtgggc tggccgatca ttctgttcct gattgccgaa
     9661 cgtctgcgta acctcggtcg ctacaccttt gccgatgtgg cctcttaccg tctgaaacaa
     9721 gggccgattc gtattctttc ggcctgtggt tctctggtgg tggtggcgct ttaccttatc
     9781 gcccagatgg tgggcgcagg taaactgatc gagctgctgt ttggccttaa ctatcacatt
     9841 gcggtagtgc tggtcggcgt gctgatgatg atgtacgtcc tgttcggcgg catgctggcg
     9901 accacctggg tacaaattat taaagccgtg ctgttgctgt tcggtgccag ctttatggcc
     9961 tttatggtga tgaaacacgt cggctttagc ttcaacaatc tgttcagcga agcgatggcg
    10021 gtacacccga aaggtgtcga catcatgaag ccgggcgggc tggtgaaaga tcctatctcc
    10081 gcgctctctt tggggctagg actgatgttt ggtacggcgg gcttgccgca tattctgatg
    10141 cgcttcttta cggtcagcga tgcccgcgaa gcacgtaaga gcgtgttcta cgccaccgga
    10201 tttatgggct acttctacat cctgaccttt attatcggct ttggcgcgat catgctggtc
    10261 ggtgcgaatc cggaatataa agacgcggcg ggccatctga ttggtggtaa caacatggca
    10321 gcagttcacc tggccaatgc ggtgggcggc aacctgttcc tcggctttat ttcagcggtt
    10381 gctttcgcca ccattcttgc ggtggttgcg ggtctgacgc tggcgggcgc atcggcggtg
    10441 tctcacgacc tgtacgctaa cgtcttcaaa aaaggtgcga ccgaacgtga agagctgcgg
    10501 gtatcgaaaa tcaccgtact gatcctcggt gtgattgcca ttatcctcgg cgtgctgttt
    10561 gagaatcaga acatcgcctt tatggtgggg ctggcgtttg ccatcgcggc gagctgtaac
    10621 ttcccgatca ttctgctttc catgtactgg tcgaaactga ctacccgtgg cgcaatgctg
    10681 ggcggctggt taggactgat tactgcggtg gtgctgatga tcctcggccc gacgatttgg
    10741 gtacagatcc tcggtcacga aaaagccatt ttcccgtatg aatacccggc gctgttctct
    10801 atcagcgtgg cattcctcgg tatctggttc ttctcggcaa cggataattc agcggaaggt
    10861 gcgcgcgagc gtgaactgtt ccgtgcgcag tttatccgct cccagactgg ctttggcgtt
    10921 gagcagggcc gcgcgcatta atctctcccc ttccccggtc gcttgatcgg ggaatttctt
    10981 cagaacatca tcctggccac cagcggcgaa accaatacca tcaatacacc tgaaagcatc
    11041 atgaccaggc ttgcaactac gccttcttgt tgacccagtt catacgaccg cgcagtcccg
    11101 gcaccatgag atgccgcgcc gaatccagca cctttcgcca tcccttcgcg aatagagaga
    11161 cgtaaaaaca acacgtcgcc aatcgccatg ccaaacacac cggtgaccac cacaaacagc
    11221 gccaccagat caggctgtcc gccgagcgac tggctcgctg ccaaagcaaa tggcgtagtg
    11281 accgaacgca ccgccaggct acgctggatc tcatctggca gggtaaacaa tcgcgccagc
    11341 cacactgagc tggtcactgc caccaccgtt gccgtcagta cgcctgccgt caacgacatc
    11401 cagtgacgct taatgatcgc ccgattgtca tacactggta ccgcaaaggc gatggtggcc
    11461 ggacctaata gccatagtaa ccagtgcgct tcgccaatat agttttgcca ggaaatattg
    11521 ccaaaaacca gcagcataac cagtagcgta ggcgtcagca ccagaggcat caatggcaat
    11581 ttacgaaagc ggcgataaag gcgtttgtta agaaaataga taactagcgt aattgccaga
    11641 cagagcatac ttagctgaaa attagccacg gtttaacctg cgcatttcat agcgatagac
    11701 tttttccact acccaggcag tcgtgcccag caccatcacc gtactcagcg cgatcacagc
    11761 aaaaatacgc cagccatcca ccagtaatag ttgtgcatag ttcaccacag ccaccacggc
    11821 agggacaaaa aacagcaaca tttcggccaa tagccaacgc gccccggctc gaacccattg
    11881 cagaggaaga atgcgacaaa caatcagcgc cagcatcagg atcatcccca ccaggttggc
    11941 aggcagcggc aaatgcagcc aggagacgag atactgcgca aaaataaata aacctgcata
    12001 cagcacaacc tgaaaaaata cctggataga ttgcactacg gcaggggtaa tgcggcgaag
    12061 cgccacgggc atgagaggat atcctgaaaa taaaacagaa tggtaagtat agagagcgct
    12121 cgcccctgac tgaaatgaat taaaatcatt gcaggtatag tcaaagggaa tagttatgga
    12181 tatcagaacg ctgcgttatt ttgtcgaagt cgtgcgccag caaagcttca cccgcgcagc
    12241 ggagaagttg tttgtcaccc aaccaactat cagcaagatg ctgaagaatc tcgaagatga
    12301 gctgaactgt atattgttga tccgcgatgg tcgtcggctg cttctcactg atactggtcg
    12361 cgtcgtattt gagcgcgggc tggctttact ggctgagttt cggcaactgg aagctgagct
    12421 tagcgatatc aatcagctta aaaccggcgt tttgcggctg ggaatcccgc cgatggttgg
    12481 gatgttgatg gccgggccga ttggcctttt tcgccagcgt taccccggcg tagaattgaa
    12541 aatttcggag tttggtggtc tgacggtaca acaagcggtg atgaacggtg aactggatat
    12601 ggcactgaca gccctgccag tagaagaaag cggcctgaca acgttatcgc tgtttagtca
    12661 tccactatgt gtactgacgc cacattcagg taaatggctt gaatgtgatg ccgtctcacc
    12721 ggagacattg gcggaacatc ctctacttat ctataacgaa gattttgcct taagtaagca
    12781 attgatgaag ttattcgatg aacatggcgt taagccgaga attgcagttc gcagcgggca
    12841 atgggatttt ctcgccgcga tggttcaggc cggtgtcgga atagccatat tgccggaacc
    12901 catttgccag cgattagata aaaacacctt aaaatggcta ccgctggaaa gcgaactaag
    12961 ctggaaatta ggaatgattt ggcgtgaggg cgtttacctt tcccactctg cccacgcgtg
    13021 gctgagctgt tgtgaaggat actggttaac gccagagtaa gtcgggttgg cgggctcgcc
    13081 cgccaaccat gcagattact gattttcctc aatcagcaac gcttcaagca aatcgagatc
    13141 gtgcagtaat ttttgcagcg tttcgttact gatctcccgc gtggcgcgca ggtggtacag
    13201 ttcagcacgt tcagaacgca atgccgccag acggaagcga cgctccaggt tctcttccag
    13261 gattgaactt tctacatcat tacgtccatc ggcgcgacga cgcaggttac caatgacgcg
    13321 agaactaacc tctgtgagca gctggttatc gatattctct tcggtatccg ccgccaaacg
    13381 ctcctccatt ttctggatcg ccacaatcgc cacttctgcc gttgccgctc gtgcaatacg
    13441 ttcctctttc aattgttgcg aatggtccgc gacttctatg tgttgtagca gaataggcaa
    13501 catcaccacg ccgacaaaca acgagaagag aatgacgcca gccgccagga acaccaactc
    13561 atagcgcgca gggaagacgt taccatccgg caagagaagc gggatagaga gcacaccggc
    13621 cagagtgatc gccccacgca ccccggcgaa agacgcgatc aggatttctc gtgtagtcca
    13681 cgaaccaaac tccatcggct tcttcttcag gaagcggttg ctgaactttt tcatcgtcca
    13741 cagccagccg aaacggacca gcatcagcgc cgcatatatc agaataatat cggtaaacag
    13801 catccagatt tcgacgttag gatcgatttc tgccgccatc agcgacgtct ccagaatccc
    13861 cggcaactgc agacctaaca gcaggaacac catgccgtta aagacaaatt ccagcatcgc
    13921 ccaggtgctg tttgcgcgca ggcgcattgc cagcggcgca cggcgcatca caccggagcg
    13981 ggtgatggtc atcccggcgg caaccgcagc gaggatgccg gaaacgccaa tatgttccgc
    14041 aatcagataa gaagcgaatg gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc
    14101 accgccccag cggctgagga atcgcagcga gcgaccatac agccagctca ccacaaaacc
    14161 ggcgagaata ccgcctattg ccactttcat aaattcgacc gtcgcaccgc cgacggtgaa
    14221 gatcattgtc cccatcgcca ctgccacggc aaacttcaac gacaccaggc cggaggcgtc
    14281 attcatcaac gcttcgccct gcaaaatgcc catgattttt ttcgggatgc gcccctcgcc
    14341 aacaatcccg gagagcgcta cagcatcggt cggagaaagc accgcagcca gcgcaaaggc
    14401 ggggatcagc ggaatgcccg gcaccaccca gtaaataagg aagccaatgc cgaccacggt
    14461 gaccaccacc agcgccagcg cgaggccgaa aatctctcgc ccatgttcga gaaattcacg
    14521 ggtcggcgtt ttccaaccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag
    14581 ttcaggatca aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc
    14641 gatttgcata agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag
    14701 cgagaccacg agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc
    14761 gatgtttttt atgatctgag aaaggcgtta tgcctgaatt ctcatttttt cagaatatcg
    14821 caaaagctca cacaatgtgc ggagaaaata aaaacgggca gccattgcca cccgttttca
    14881 gaatcctatg taagattaga tagcccagcc acccgcatag aaggtcacca gcgccacggc
    14941 aataacgacc gtaccgatgt tcaacttgcg ccattcgccg gaaaccagac gaccaatcac
    15001 cagagtcgcg aagccaatca tgatgcctgt tacgatgtta caggtcagca cgatgaatac
    15061 cgccgtaacc agacccgcca tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt
    15121 gctcagcatc agcaggccaa cgtacatcag cgccggagcc gttgcgtacc ccggaacgag
    15181 gtaagagagc ggagagagga acagaatcag caggaacagc acgccaacgg tgatagccgt
    15241 caaaccggtt ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg
    15301 agctgcaccc accaggccag aaaaaacgct gctcatggag tcggtggtca gtgctttccc
    15361 gccgtcgatg atctgcccat ctttatccag caggttcgcc tgaccggcga cggcacggat
    15421 agttccggtg gcatcaaata ctgccgtcat caccagtgcc agaacacttg gcaggactac
    15481 aggattcagc gcgcccataa tgtccaggct gcctatcagt gaattgccgt tttcatcgct
    15541 caatgaaggc atggcgaaaa cgccggagaa atggacgtta ggatcgaaga tcaaaccgac
    15601 aattgagata ccgataatgg tcagcagaat gccgccaggg actttcagtt tttccaggcc
    15661 gataatcact gccagcccca ccaacgacat catcaccgga aaggtcgtaa aatcccccag
    15721 cgcgacgggc agaccatcaa gcgggttttt aatcaccaga ccgacaccat tagcggcaat
    15781 gagcagcagg aacagaccga taccaatccc cgtgccgtgc gccacaccgt gcggcaagtt
    15841 gcgcaaaatc cagctacgga tacccgtggc agaaattacc gtaaacagca cacccatcag
    15901 gaacacggca cccagcgcga caggtacgct aatatgttgc cccagcacca gactgaatgc
    15961 ggtaaacgcc gtcagagaga tggcgcaacc aatcgccaac ggcagattag cccacagacc
    16021 catcacgata gaaccgagtc cggcaaccag acaggttgca acgaaaactg ccgcaggcgg
    16081 gaagcccgct ttacccaaca tgcctggaac gacgataacc gagtagacca tcgccagaaa
    16141 cgttgttaac ccggcaacca cttcctgacg gacagtgctt ccacgttgtg aaattttaaa
    16201 ccaggcgtcg agtgaaccgc cggtacgcgc tgatggcgta gacataagaa aacatcccct
    16261 gagagtttaa ttttcgtcaa gatgcgtggc ggacaatccc ctgccagcga aacgttatct
    16321 tcctgctcca ggtttgcgac aatttgctgc gtcgcaaaaa aagcaaacgt ttagctccgc
    16381 gcatacaaaa cgggtggtaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc
    16441 aaccgaagtt tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg
    16501 atgcgcaaac gttatcgtcg atgcgcaaga ttgcagcgtt atcgagccgt ataccgttca
    16561 ggattgcgat aaaaaatcgt ctgagatgac aaaaatattg cggctaaaaa gtgtactttt
    16621 acactggcga agaaacgccg gtaacataca attaaagcat caacaccaac cggaacctcc
    16681 accacgtgct cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt accagcggga
    16741 tagagagaaa gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg
    16801 ttcatcttcc agtaagcgtg cgccggtacc ttcttctcct aagcggtcgc ccgggttacg
    16861 caacgggcaa tcactgcgcg aaaggcagcc acaaccaata catccatcca gttcgtcacg
    16921 cagcgccact aaggtatgaa tgcgccgatc caactcttct cgccattggg acgaaagctg
    16981 tttccactct ttcgcactta acgtatgccc ttctggcaac acgccaaacg cttcacgaat
    17041 ggttgcgagc ggaatgccaa tacgctgagc aattttgata attgcgacat atcgcaacac
    17101 atcacgttta tatcgccgct gattgccgct attacggata ctggtaatca accctttgct
    17161 ttcatagaaa tgcagcgccg ataccgccac accgctgcgt ttcgccactt cgccgggtgt
    17221 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
    17281 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
    17341 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
    17401 gccgcttaac attgatgtcg tcgcaaaaaa atctggctat tcaaagtggt acttgcaacg
    17461 gatgttccgc acggtgacgc atcagacgct tggcgattac attcgccagc gccgtctgtt
    17521 actggccgcc gttgagttgc gcaccaccga acgtccgatt tttgatatcg caatggacct
    17581 gggttatgtc tctcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
    17641 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaattccc gcgcgggtaa
    17701 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcgaaga accatccctg
    17761 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc gcctcagttt cgacaccttc
    17821 agcaatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
    17881 actggttttg tgggtggtca gcgtctcaac aaacgattta tcgattttca aaatatcgac
    17941 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
    18001 ttcgtaaccg gccttgcgga aagccagaat aatcggcgtc attttgtcga catcaagaaa
    18061 tgcatgttca gtcacttcaa atttaatttg ctgcgggcgc accgcatgtt gctctgtttt
    18121 ctgattggtt cgcgctatca accgtgaggt atgaaaatcg gaggctgaca ggttaataga
    18181 aacatagcga tcggcatgtg ttgccagata cgcgcccaaa tcgcggaaga cattatcaat
    18241 aacataatca gttacctgtt cgatcatccc ctccttttct gccagcggaa taaactctgc
    18301 cggattcatt acttgcccct gctcaccggg ccaacgtaac aacgcttcag cgccgataca
    18361 tttttctgtt ttgatatcga ttattggctg gtaataaagg aaaagttgat gtttttcgag
    18421 ggcgcgttgc aatttgcgtt tgggagacaa atagttttgt cgaatacgta gccagagtaa
    18481 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
    18541 atgggttata agacgttgat atgaagtcgc aacaatggcc gcaattgggc gttttgtcga
    18601 ataaactgtc gcatataaat agccatttcg ttgtacggtt aaatcattca aacgaatcag
    18661 cggagaaaac gttgctgccg aggcctcgtt gcttaacgag aaaaaggttt tcgtcaccgt
    18721 atcatacaca ccccattgca atgtcggatc atcagacatc acttcactcc agaagagagg
    18781 gttaatgacc gctacataat tcccccgctg catataggtc attttatagc ctgagaaaaa
    18841 aggcgtatca cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
    18901 aatcgtatag ccatttacag acgctatcag cgatgagcat aaaaaatggt tatcacgagc
    18961 ataaatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
    19021 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
    19081 acgaatgacc agctcagatt tatccaacgc cagctgagca aaagttcgga gctgattatt
    19141 cacttctgat tttgcccaaa agaaggcaat ccatagcgac agaatgatgg gaaagagaac
    19201 taaaaagata atgcccggaa acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
    19261 caattccctg cctgtttata cccggttatt gcaaggtgaa accctggcta ccgacgtctt
    19321 tagataaatc ttacggtata aatagcactt ttgcctgata taaaattgag acctttttaa
    19381 tcgtcaccag gatgacgata attcattgat cttaaaaatt aatcaccttc aggaatgcag
    19441 aggcggcggg aagatatacc cttaatgccg ctgaaaaaac acataatctg tacggcaata
    19501 aaagtcacgg cctaatctgg ctaacaaaca accaattgtg atatagttca caaaattcat
    19561 gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtatc
    19621 acgcctcccg tgaacaacgc cgcgctgtcc agggcatcag ctctttttcg aggaattgtt
    19681 tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa
    19741 tgatgtttct ggccagcacg ctcaacatcc gttttcgtcg ggctgattat gtcgggcttg
    19801 cagtgatcag cagcggtttg ggcgtagttt ccgcctgctg gttcgcaatg gggttgcttg
    19861 gcatcacaat ggcggatatc accgccatct ggcacaacat cgagtcggtg atgatagaag
    19921 agatgaatca gacaccgcca caatggccaa tgattttgac ttgatatgta gaagcctcca
    19981 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
    20041 accctattgt tttaaagaca aatcagaacg gaatatcgtc atcaaagtcc atcggcggct
    20101 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
    20161 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
    20221 tattgccacc tgcaggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
    20281 caacgttcac cacgacttct gtggtatagc gatcctgacc ggattgatcg gtccatttgc
    20341 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcta
    20401 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgctct ttcatctcgc
    20461 cggtggcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
    20521 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
    20581 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa atcgttctga ataagtgtaa
    20641 acgcgcgatt gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa
    20701 acaaacctgg ccagacattg ttacacaaca ctccgggtaa tgcattccaa tactgtatat
    20761 tcattcaggt taatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac
    20821 cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc
    20881 cgcacccata atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg
    20941 accgggcttt cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg
    21001 cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggag
    21061 aagccggacg tcgaccatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg
    21121 acgtctcata acccgcgctc tacggtgggg acaatcaccg aaatccacga ctatttgcgt
    21181 ctgctgtacg ctcgcgttgg tgagccgcgc tgcccggacc acgatgtacc gctggcggcg
    21241 caaaccgtca gtcagatggt ggataacgtg ttgtcccagc cggacggcaa gcgtctgatg
    21301 ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg
    21361 gcaagccagg gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg
    21421 ccgaaactgg aactgcaaaa gaaacatacc attgaagtgg tggttgatcg cttcaaagtg
    21481 cgtgacgatc ttacccaacg tcttgccgag tcgtttgaaa ccgcgctgga gctttccggt
    21541 ggtaccgcgg tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttctccgcc
    21601 aactttgcct gcccgatttg cggttacagt atgcgcgagc tggaacctcg cctgttttca
    21661 tttaacaacc cggcgggagc ctgcccgacc tgtgacggac ttggcgtaca gcaatatttc
    21721 gatcctgacc gcgtgatcca gaatccggaa ttatcgctgg ctggcggtgc gatccgtggc
    21781 tgggatcgcc gcaacttcta ttatttccag atgctgaaat cgctggcaga tcactataag
    21841 ttcgacgtcg aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac
    21901 ggttctggca aagaaaatat tgaattcaaa tacatgaacg atcgtggcga tacctccatt
    21961 cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacggaa
    22021 tccagcgcgg tacgtgaaga attagccaag tttatcagca atcgtccgtg cgccagctgc
    22081 gaaggaaccc gtctgcgtcg ggaagcacgc cacgtgtatg tcgagaatac gccgctgccc
    22141 gccatctccg acatgagcat cggtcatgcg atggaattct tcaacaatct caaactcgca
    22201 ggtcagcggg cgaagattgc agaaaaaatc cttaaagaga tcggcgatcg tctgaaattc
    22261 ctcgttaacg tcggcctgaa ttacctgaca ctttcccgct cagcagagac actttccggc
    22321 ggtgaagccc agcgtattcg tctggcgagc cagattggtg caggcctggt tggcgtgatg
    22381 tacgtgctgg atgagccgtc tatcggcctg caccagcgcg ataacgagcg cctgctgggt
    22441 acgcttatcc atctgcgcga tctcggtaat accgtgattg tggtggagca cgacgaagac
    22501 gcgattcgcg ccgctgatca tgtgatcgat atcggtccgg gtgcgggtgt tcacggcggt
    22561 gaagtcgtcg cggaaggtcc gctggaagca ataatggcgg tgcctgagtc gttgaccggg
    22621 cagtacatga gcggtaaacg caagattgag gtgccgaaga aacgcgttcc ggcaaatccg
    22681 gaaaaagtgc tgaaactgac gggcgcacgc ggcaacaacc tgaaagacgt gacgctgacg
    22741 ctgccggtgg gtctgtttac ctgcattacc ggagtttcag gttccggtaa atcaacgctg
    22801 attaacgaca cactgttccc aattgcccaa cgccagttga atggtgcgac catcgccgaa
    22861 ccagcaccgt atcgcgatat tcaggggctg gagcatttcg ataaagtgat cgatatcgac
    22921 caaagcccaa ttggtcgtac tccgcgttct aacccggcga cctataccgg cgtgtttacg
    22981 cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga
    23041 cgtttcagct ttaacgttcg cggcggacgc tgcgaagcct gtcagggcga cggcgtgatc
    23101 aaagtggaga tgcacttcct gccggacatt tacgtaccgt gcgaccagtg caaaggcaaa
    23161 cgctataacc gtgaaacgct ggagattaag tacaaaggca aaaccatcca cgaagtgctg
    23221 gatatgacca tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct ggcacgtaag
    23281 ctgcaaacat tgatggacgt tggtctgacg tatattcgtc tgggtcagtc cgcaaccaca
    23341 ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc
    23401 gggcagacgc tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag
    23461 caactgctcg acgtactgca taaactgcgc gatcagggca ataccattgt ggtaattgag
    23521 cacaatctcg acgtgatcaa aaccgctgac tggattgtcg acctgggacc agaaggcggc
    23581 agtgggggcg gcgaaatcct cgtctccggt acgccagaaa ccgtcgcaga gtgcgaagct
    23641 tcgcatacgg cgcgcttcct caagccgatg ctgtaatcgt taaggccgct ttctgagcgg
    23701 ccttcttctt tcagagttgc accagcaatt tacgtttttc ttccggcagc aaattcaccg
    23761 cctgctgata agacgcatcc accagataat agatttgcga atccggcagc gatccgtcga
    23821 gatacacggt gctccagtgt gctttgttca gatggcggct tggacgcaca tcgctgtgct
    23881 gctgacgtag cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt
    23941 ctacttcttt caccatcgca aacagcacat cttccacttt gatctgcgtc gctttccagt
    24001 cgttatgcac gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccaaaa
    24061 tggtcatttt ttactcccct tgaagtgtcg cgatgatgcg acgcgatccg ccgtggatgc
    24121 gatgttcccc cagccagatg ccttgccagg tgccggtctg aatacgccct ttatgcaccg
    24181 gcaatacaag cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc
    24241 cctcatagtc atgctcataa tttccgttgt cgggaacggc gcggaggaaa aaacgctcca
    24301 tgtcgtggcg tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt
    24361 gcagcaacag atgcagtaaa ccgatgttaa cgcgcggcat atcagccagc tgattcagaa
    24421 tttcatccgt taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat
    24481 accacatgtg ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag
    24541 gataaaggtg tgattaaaaa aacagcattc gggagagtat tacgctctcc cgctctgtca
    24601 gtattctgaa ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta
    24661 ggtagagttg gaggcgcgca gaatgcggat accacgagcg ccaacatcgc gtgcggcggt
    24721 aatatcgtta tcggaatcac cataaaaaat tcggatattt ttatcctgca gccattgcga
    24781 ttttgtattt tgacctggtt tatcgcccgc aaagatcacc ggattcatgt tggtggcagg
    24841 aatatgaaaa ttatccgcca gcgtttttga aaccgtttct gttttggtcg ggctacgacc
    24901 agtcacaaag aagatcgcgt caccgcggcg tacatgcata tcaatcagct ggcgagcgac
    24961 ctcttttgga atgctgaatt catcccagcc attgttcatt ttttcccaga acacaggatt
    25021 tttcagataa tcttcgcttt ctggtgagaa ggtttttttg ccgcgccaga aacccggact
    25081 ggaaaaaagt accgtatcat cgatatcaaa ccccaccgcc attggcggac gtcctgcgag
    25141 gctattttca atttgtgcaa ccgaaaccca atgaattggt gcctgttcag cgagtttggc
    25201 tacgttagta cccggattaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt
    25261 tagagcgaac aataagcaaa cggcactcaa tgcctgtgtg atcttgcgca tatttttccc
    25321 taaatagtca gtaagttgaa acttattatt gtgcgtttgt tgaaaaaacc atctgaccat
    25381 aacgccaacc gcaggggaaa ggaaggatta tctgcggatt tgtcacataa aaagagcatg
    25441 atcacatttg gaatacctgt gcgataaacc acacattgcc acggttattc tgcttacaca
    25501 ttcacacaat ggcactcttt tggttctcgc aaacattgtt cagggaatat actgatgata
    25561 aatataaatg cattcaatgg atgatgaatg aaaatatcgt ggaattatat atttaagaac
    25621 aaatggcgat ttcacattac aagcatttca ctttttctta tcatgctcgc ggtttcaatc
    25681 gcttttttgc acttgcgttt taataccttg tccggtaccg ataaaatgcg gcttgaaatg
    25741 tataagtcca cattatattc caccatcgag caattttatg ttttacccta tatgctctca
    25801 acagaccata tcatccgtca ggcggtaatt acgcctgacg atatgacgtc cagcgaactc
    25861 aatcaacgaa ttgcacattt caatactcaa ctcaaaaccg cagcaatatt tattctggat
    25921 acccaaggta aggccatcgc ttctagtaac tggcaggacc ccggcagcta tgtagggcaa
    25981 aattatagct atcgccccta ttataaacac gccatgtctg gcttaaatgg acgcttttac
    26041 ggtattggta gcactacgaa tacaccggga ttcttcctct ctacaagtat aaaagataaa
    26101 ggaaaaattg tcggtgttgt agtagtaaaa ataagtctta atgaaattga aaaagcatgg
    26161 gccgaaggtc ctgaaaatat tatcgtgaat gatgaacatg ggattatatt tttaagttca
    26221 aaatcgccat ggcgaatgcg aacactgcaa ccgttacctg ttcaggcaaa acaaaaacta
    26281 caatctaccc gccaatatag tctcgacaat cttttaccgg cggattatta tccctgttat
    26341 accgtgagca attttacttt cctgaaagat aaaaaagaac aactctgttt attcccgcaa
    26401 tattatacgc aacaaatagc cattccagaa tttaactgga aaatgacaat tatggtcccc
    26461 ttagataacc tgtactggtc atgggctatt tcgttagtca ttacactaat tatttacctg
    26521 ctgtttttgt tatttattaa atactggaga atgcgatctc atgcacaaca attattaaca
    26581 ctggcgaatg aaacattaga aaaacaggtt aaagagcgta catctgccct ggaattgatc
    26641 aatcaaaaat taatacagga gataaaagag cgcagtcagg ctgaacaagt attacaaatt
    26701 acgcgtagtg aactggcaga gtccagcaaa ctggcggcgc ttggacagat ggcaaccgaa
    26761 attgcccatg aacaaaatca accgttagcc gccattcacg cacttactga taacgcgcgt
    26821 actatgctaa aaaaagagat gtatccacag gttgaacaga atctgaaaca cattatttcc
    26881 gtgattgagc ggatgacgca gctcatttcc gaacttaaag catttgcctc gcgccatcgc
    26941 gtacctaaag gttctgccga tgtcatcaaa gtgatgtata gcgccgtggc gttacttaat
    27001 cacagcatgg agaaaaataa cattgagcga cgaataaaag ccccatccat gccgttattt
    27061 gtcaattgcg atgaactcgg tcttgaacag atattcagta atttaattag caacgcctta
    27121 gattctatgg aaggtagctc ttacaaacga ctggatatcg ccattcgcca ggcaaataac
    27181 aaagttatta ttaccattaa agacagcggt ggcggttttg cacctgaagt tgtcgatcgc
    27241 atatttgaac cattttttac cactaaacgt agaggaatgg ggttgggact ggcaatagtc
    27301 agcgaaattg tccgaaattc gaacggcgca ctccacgcca gtaatcatcc tgaaggcggc
    27361 gcagtaatga cattaacctg gcctgaatgg ggagaaaaac atgaataata aattcagtga
    27421 aattactata gtttatattg aggatagtga cgatgtacgt tttgcctgtg aacaaacgct
    27481 cacgctggct ggttatcgcg ttatttcctg ttgcgatgca gaacacagta ttccccttat
    27541 tcaaagtcag gccaacatta ttatactgac cgatgtcagg ttacctggaa tatcaggact
    27601 agagctcctc agttacatca acgaaatgga tagcaaaatt ccggttattt taattaccgg
    27661 acacggtgac gtggaaatgg cggtggatgc catgcgtaat ggtgcattcg atttcattga
    27721 gaaaccaagt agctcagata aattattgag tattattgcc agagcggtag aaaaacggcg
    27781 attggtttta gaaaatcagc aattactggc gaatctgcaa caggaaaatg gcccggtgct
    27841 gattggtcgt agtccgcaga tgcagcaact gcgtaaaatg atccttaatg tcgcagatac
    27901 cggtgccgat gtccttattt atggtgaaac aggttgtggt aaagaagtcg tcgccagaat
    27961 gctgcatcac tggagtaccc gccgtcaggg gcagtttgtt gccttaaact gtgcaggctt
    28021 accagaaacg ttgtttgaaa gcgaaatatt tggtcatgag gcgggcgcat ttaccggtgc
    28081 ggttaaaaag cgtattggaa aaattgaaca tgcaaatggt ggtacgttat ttctcgatga
    28141 aatagaggga atgccatcag gaatgcaggt aaaactgcta cgtgtattgc aggaaagaac
    28201 cattgaacga ttaggtgcca atcaattaat tccggtgaat tgccgtgtta ttgcagcgac
    28261 caaggaagat ttactgcgtc gcagcgaaga acatctgttt cgtctcgatc tctattatcg
    28321 tctgaatgtg gttagcctga atattccacc tctacgccag cgacgagaag atatccctga
    28381 actgttttac tggttcgcca gtcaggcggc acaaaaatat aatcgtcctc tgcctgatat
    28441 ttctcccatg ttactggcct ggttgcagtc acaaagttgg cctggcaatg tacgtgaact
    28501 taaacacaat gcagagcgtt ttgttctcgg tttactgacc caccaccagc cagtgccaat
    28561 gactcagcaa gaagagagtg gtcttaccgc ctgcattgat gctttcgaga aaaagttaat
    28621 cgaagatatg ctgcgacaaa cggaaggcca ggtcagtttg acagcgcggc tattacagct
    28681 gccgcgcaag actttatatg acaaactcaa caaacatcag attcaaccgc aagtttatcg
    28741 accagaatca tccagctaat gctgattcct gaggatgtaa ccccaaatcg atacctttgc
    28801 gggcgtaatg ttcaactacc caggaccaga atgataccgg gatcgtaatc atcttcgcat
    28861 tgcgttcccg gttggcagct tctggcatac cggggtaaat caccggattg ccgccaggtt
    28921 gaggctgcgt agctaacaga taatccgcaa agaactgccc tttctcccct gccagatcgg
    28981 tgaattgatc aggatcgata atcaacgcaa aaagatgatt atgcgcagat ccatccgtct
    29041 gatgatcttc ggcgatcgtg tcaccaccag ataagatccc cgccatcatt tcaatcatca
    29101 gtgccaggcc aaatccttta tgctcaccaa atggcagtaa cgcccctgga ggattgctga
    29161 ataacacccc aggatcattg cttggattcc cctgtgcatc aatcaattgt cccggcgcgg
    29221 cctgcttccc ggtggacgac attacccgtg ctttatttag cgccatgcta ctggtggcaa
    29281 aatcaaccag aatcggcgga cgcccatcac tgaacggcca ggcaaagcag aaagggttag
    29341 tggtcatacg cggttccgca ccgccccacg gggcgaccag tgcaggacgc ccggtgacgt
    29401 tactcatggc cagcatcgcc aggccttgat cagcaaccat ttgcccgtaa tgccccatac
    29461 gccctaaatg atgcgcatgg cacaacgtta acaggcaaaa accgcggtgt tttgtttgtt
    29521 cgatggcagc atttaccgcc acttttccgg cgtgttgacc aaagccattg tgggcatgaa
    29581 aacgctgtaa attaccttca cttgttaagc attccgggcg ggcattcgcg gttacatcgc
    29641 cacgagcgat attttccaga tatttcggta gcagcgtgac gccgtggctg gcaaagccat
    29701 aacgttccgc ttcaatcata tgttccgcca catcctgagc gatatcggca tcacagccat
    29761 taagttccag cagcccggca gctaaccaac gcactttttc aacagcaatt tgtaatgaca
    29821 tcgttggttt cctcacatta gacctaaaat tttccaccag gtggcggcaa acagtagcaa
    29881 caacaggtag ccgattaccg tcaggtacag tcccacacgg gtaaactggc gtggcgtgaa
    29941 ggtatccgta cccatacaca ccatattttg tggcgcgtta atcggcagga tgaagccaaa
    30001 actgacactg aaggcgagca gaatggtcat gccgaccggg ttaacgccga gttctggcgg
    30061 cagactgctt aacaggctaa tcaggatggg cagcaatgcg gcagttagcg cagtggcact
    30121 ggcaaacccc aggtgaatga ttatcaagaa agcggcgaga atcgcgaaga tggcaagtga
    30181 tggtaaaccg tcaagaccaa aacctttcac cacatagttc gccatccatg aagctgcctg
    30241 tgtatcaagc aacgtggacc ccaggctgat accaatacca aacatcagca acgtgcccca
    30301 ctgcacacgt ttttcgacct ctttccagct cattacaccg atgcctggca gcaacataat
    30361 cgccagtccg gcaagggtga cagaggtggt atcaatactg tgtaattttc cgccagtaga
    30421 ccaaaagagc agtaacaaca gcgaaatacc gatcaagcgt ttttctttac cggtggttgg
    30481 ccctaactct gcgagtgctt ttttaatggc ctcgctcccc ccttcaacag cttccgtttc
    30541 tggcggcaac aatttacgtg cgaggaaata gaggatcacc gacatggtga ggctccaggg
    30601 cgcgcccgct aacagccagt caagccagct gacagaatgc ccggcgccga aagttttatt
    30661 gataaaaccg atagaaagca ggttttgcgc cgacgaagtt tgaataccaa cgttccagat
    30721 actggtcgcc tgggcgataa caatcatcat tgacgccgct aaacgtgaat gtttatccac
    30781 cttaaatgcc gcaatgaccc ccatcataat cggcaccacg caagccgtac gggcagtggc
    30841 actgggtacg accaggctca ggacgatggt caccacaatg gcaccgataa taatgctgcg
    30901 gctactggca cctattttcg acatagtaaa cagcgcaata cgtttatcca gcccggtaat
    30961 ggtcattgcc gccgcgataa acattgctgc tgccaccagc gccagagcag aattagaaaa
    31021 acccgacaga gtcattttca gcgctttcgc ggtgccaagg atcgtgtccg ggtgattcat
    31081 atccggggca aaaccaacca taaagataat cagtgcggaa ataacaattg cactggcggt
    31141 gtaatccatc gcttcgctga tccagacaat aatggcaaac accagaatgg cgatcatatt
    31201 ttgtccggcg acgggcaagg tatcgcccag aggcagaaga gtaataataa ttccggcaat
    31261 gatcgccaac cacaggccgg gattagggat aattcgacgc ttttgtggtg cgccatgtga
    31321 tattgatgac atattcacct cattgtgaaa aataaaaaac ctgcctggct attgcaggag
    31381 ctatgccatc tttaattttt taataatgct ttgttattta atgattttat tatttattct
    31441 tttt
//
LOCUS       DAPQZD010000045        30722 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPQZD010000045 DAPQZD010000000
VERSION     DAPQZD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.045"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..864
                     /gene="tuf"
                     /locus_tag="RO454_001966"
     CDS             <1..864
                     /gene="tuf"
                     /locus_tag="RO454_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDY0933806.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRT
                     VGAGVVAKVLS"
     gene            1094..1477
                     /gene="secE"
                     /locus_tag="RO454_001967"
     CDS             1094..1477
                     /gene="secE"
                     /locus_tag="RO454_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDY0933807.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1479..2024
                     /gene="nusG"
                     /locus_tag="RO454_001968"
     CDS             1479..2024
                     /gene="nusG"
                     /locus_tag="RO454_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDY0933808.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2183..2611
                     /gene="rplK"
                     /locus_tag="RO454_001969"
     CDS             2183..2611
                     /gene="rplK"
                     /locus_tag="RO454_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDY0933809.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2615..3319
                     /gene="rplA"
                     /locus_tag="RO454_001970"
     CDS             2615..3319
                     /gene="rplA"
                     /locus_tag="RO454_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDY0933810.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3611..4108
                     /gene="rplJ"
                     /locus_tag="RO454_001971"
     CDS             3611..4108
                     /gene="rplJ"
                     /locus_tag="RO454_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDY0933811.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4175..4540
                     /gene="rplL"
                     /locus_tag="RO454_001972"
     CDS             4175..4540
                     /gene="rplL"
                     /locus_tag="RO454_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDY0933812.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4860..8888
                     /gene="rpoB"
                     /locus_tag="RO454_001973"
     CDS             4860..8888
                     /gene="rpoB"
                     /locus_tag="RO454_001973"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDY0933813.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8965..13188
                     /gene="rpoC"
                     /locus_tag="RO454_001974"
     CDS             8965..13188
                     /gene="rpoC"
                     /locus_tag="RO454_001974"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDY0933814.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="RO454_001975"
     CDS             complement(13430..14563)
                     /gene="thiH"
                     /locus_tag="RO454_001975"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDY0933815.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="RO454_001976"
     CDS             complement(14560..15330)
                     /gene="thiG"
                     /locus_tag="RO454_001976"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDY0933816.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="RO454_001977"
     CDS             complement(15332..15532)
                     /gene="thiS"
                     /locus_tag="RO454_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDY0933817.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="RO454_001978"
     CDS             complement(15516..16271)
                     /gene="thiF"
                     /locus_tag="RO454_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDY0933818.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="RO454_001979"
     CDS             complement(16264..16899)
                     /gene="thiE"
                     /locus_tag="RO454_001979"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDY0933819.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="RO454_001980"
     CDS             complement(16899..18794)
                     /gene="thiC"
                     /locus_tag="RO454_001980"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDY0933820.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18875..18975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="RO454_001981"
     CDS             complement(19027..19503)
                     /gene="rsd"
                     /locus_tag="RO454_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDY0933821.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            19598..20371
                     /gene="nudC"
                     /locus_tag="RO454_001982"
     CDS             19598..20371
                     /gene="nudC"
                     /locus_tag="RO454_001982"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDY0933822.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20411..21475
                     /gene="hemE"
                     /locus_tag="RO454_001983"
     CDS             20411..21475
                     /gene="hemE"
                     /locus_tag="RO454_001983"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDY0933823.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21485..22156
                     /gene="nfi"
                     /locus_tag="RO454_001984"
     CDS             21485..22156
                     /gene="nfi"
                     /locus_tag="RO454_001984"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDY0933824.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22199..22789
                     /locus_tag="RO454_001985"
     CDS             22199..22789
                     /locus_tag="RO454_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDY0933825.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22976..23248
                     /gene="hupA"
                     /locus_tag="RO454_001986"
     CDS             22976..23248
                     /gene="hupA"
                     /locus_tag="RO454_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDY0933826.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23261..23956
                     /locus_tag="RO454_001987"
     CDS             23261..23956
                     /locus_tag="RO454_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDY0933827.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="RO454_001988"
     CDS             complement(23958..24377)
                     /gene="zraP"
                     /locus_tag="RO454_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDY0933828.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            24615..25991
                     /gene="zraS"
                     /locus_tag="RO454_001989"
     CDS             24615..25991
                     /gene="zraS"
                     /locus_tag="RO454_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDY0933829.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25988..27313
                     /gene="zraR"
                     /locus_tag="RO454_001990"
     CDS             25988..27313
                     /gene="zraR"
                     /locus_tag="RO454_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDY0933830.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27310..28599)
                     /gene="purD"
                     /locus_tag="RO454_001991"
     CDS             complement(27310..28599)
                     /gene="purD"
                     /locus_tag="RO454_001991"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDY0933831.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28611..30200)
                     /gene="purH"
                     /locus_tag="RO454_001992"
     CDS             complement(28611..30200)
                     /gene="purH"
                     /locus_tag="RO454_001992"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDY0933832.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7204 a         7960 c         8471 g         7087 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggt
      721 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      781 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      841 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      901 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      961 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
     1021 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
     1081 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
     1141 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
     1201 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
     1261 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
     1321 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
     1381 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
     1441 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
     1501 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
     1561 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
     1621 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
     1681 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1741 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1801 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1861 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1921 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1981 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     2041 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     2101 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     2161 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     2221 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     2281 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     2341 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     2401 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     2461 gaacaaagac aaagtgggta aaatttcccg cgctcaactg caggaaatcg cgcagaccaa
     2521 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     2581 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     2641 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     2701 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2761 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2821 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2881 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2941 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     3001 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     3061 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     3121 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     3181 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     3241 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     3301 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     3361 cacgtacgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     3421 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     3481 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     3541 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     3601 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     3661 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3721 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3781 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3841 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3901 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3961 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     4021 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     4081 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     4141 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     4201 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     4261 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     4321 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     4381 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     4441 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     4501 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     4561 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     4621 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     4681 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4741 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4801 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4861 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4921 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4981 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     5041 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     5101 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     5161 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     5221 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     5281 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     5341 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     5401 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     5461 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     5521 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     5581 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     5641 tgtacgtaga aaaaggccgc cgtattactg cgcgccacat tcgccagctg gaaaaagacg
     5701 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5761 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5821 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     5881 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5941 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     6001 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     6061 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     6121 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     6181 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     6241 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     6301 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     6361 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     6421 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     6481 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     6541 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     6601 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     6661 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6721 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6781 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6841 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6901 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6961 gtacgggtat ggaacgtgct gttgccgttg actctggtgt aactgcggtt gctaaacgtg
     7021 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     7081 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     7141 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     7201 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     7261 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     7321 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     7381 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     7441 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     7501 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     7561 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     7621 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     7681 aagataaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7741 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7801 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7861 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7921 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7981 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     8041 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     8101 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     8161 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     8221 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     8281 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     8341 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     8401 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     8461 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     8521 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     8581 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     8641 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     8701 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8761 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8821 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8881 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8941 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     9001 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     9061 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     9121 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     9181 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     9241 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     9301 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     9361 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     9421 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     9481 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     9541 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     9601 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     9661 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9721 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9781 attaaccgta acaaccgtct gaaacgtctg ctggacctgg ctgcgccgga tatcatcgta
     9841 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9901 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9961 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
    10021 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
    10081 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
    10141 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
    10201 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
    10261 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
    10321 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggccgttca cgtaccgctg
    10381 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
    10441 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
    10501 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
    10561 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
    10621 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
    10681 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10741 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10801 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10861 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10921 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10981 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    11041 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    11101 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    11161 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    11221 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    11281 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    11341 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    11401 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    11461 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    11521 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    11581 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    11641 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caataaaggt
    11701 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11761 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11821 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11881 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11941 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    12001 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    12061 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    12121 ttgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    12181 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    12241 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    12301 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    12361 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    12421 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    12481 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    12541 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    12601 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    12661 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12721 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12781 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12841 gaagcgaacg gtaaagtggg tgcaacttac tcccgcgatc tgctgggtat taccaaagcg
    12901 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12961 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    13021 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    13081 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    13141 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    13201 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    13261 cttcacggga aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    13321 aacattcctg ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    13381 ttcatcttcc gtagcggctc ggaataaatt tacgttctta cattctgtct catggccttt
    13441 gcgatgggcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    13501 ttaacgcggc agcaaccgct tccggtctgc gatcgtcgtg cggtgagaac tgttccagct
    13561 cggggtggtt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    13621 ttgccagcgg aatcacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    13681 caatctccgg tgcaagcagc cggaaagcgc agatggcttg cactaactgg cgttcatcca
    13741 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13801 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttcggcaacc atatagcagt
    13861 caactcgcca gctgtcggaa aggccgatta gcgcgccgag gcctatctta tcaatccccg
    13921 cacgccctag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttacctttca
    13981 gatgatgatg ggcataagtc gcctcatgat atgtctcctg ataaaccatt actccatcga
    14041 ggccaagctg ttttaactcg gcatattccg cctccgccag cggttgcact tccatctgta
    14101 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    14161 tcgcctgatg ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg
    14221 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    14281 aaaatccgca ataagtacag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    14341 agctgacgac gttgccaaat cgctgacgag tcagacgttg cgctcgctgg gccagtggtt
    14401 ccagatagcc actggcggcg ggtgatagca atgccatcat gtcgtcgcgg gtgagttgcg
    14461 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggtggatgt
    14521 catcccagtc cagttgtcgc cagcgatcgc tgaaggtttt catgccgatg cctccagaaa
    14581 tccagtcagc gggctggtgg catgagcaaa atgactgcgg ctgcccggtc cggactgacg
    14641 tgccagcagg cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc
    14701 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14761 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaacggtgg cctgctggat
    14821 aataatctcc agcatggcgc gggtttccag tccctgattc gagccaatcg gcgcgccgag
    14881 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14941 gcagtaaggc agcacgacaa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    15001 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta gccagtttgt
    15061 gcctaacgct tcacgtgcca gatgggcggc gaaaatggct tcttccgcgg ttttcgcccc
    15121 ggaagtattc ggcagcaggg ccacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    15181 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agttggctgc cggaagcgcg
    15241 aatagactcc accatcagtt gtgaagaggc gaattttccg gtgccagtaa acagatgtga
    15301 atcaaacgtt ttgtccgcaa tatgtaacat ctcaaccccc tgcaataact tgaaaaagca
    15361 ggatctggtc gccatcctgc acgatatgtt gcgcccattg ttcccgcggg acgatttgct
    15421 gattaatcgc cagtgccgca cctgcttgtt gttgttcgag ttgctccagc agttcgtgaa
    15481 cagttagttc ggcgacacac tgcatcggtt gatcgttaaa caggatctgc attgcatcct
    15541 ccacataccg ggcaaccgct ggcgcggcgc aacgtcagac tgcgccactg gctcgattta
    15601 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cacttaataa cttaatcgct
    15661 tccagtgcct gtaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15721 cagttgcgtt ctggctcctg gttatctggc cacaggcagc ggtaacatcc ctgctcccag
    15781 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15841 gtgttgagtg ccacgcaggt ggcattaatc tcctggcgag tcgccatatt gtcagtacag
    15901 tcgagcacca catcggcttg tgcaaccgca tcttttaacg tctcacccgt taaccgttgt
    15961 tgtaatgcca tcagtcgaat atcgggattc aactgtgtca gtcgctgttg gctgacctgt
    16021 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    16081 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcccccgc caggtatagc
    16141 gcagcaggtg tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt
    16201 tgctgcccgt ccagagcgat atcgtcgagc aggatttggc ggctataacg cataaagtca
    16261 cggtcattca tcgccaactc ctgcaatttc cagcagctgt gccgttgcca aacgccagtc
    16321 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    16381 aggcgcgcgc gccagactga taccgccaat cgccacggtg ggataatccg ccaggcgctc
    16441 aacatgccgt gccagctgtt ccagtccctg cggtgcagaa ggcatctgtt tggtttgtgt
    16501 cggaaacaca tgtcccagcg cgatataaga aggacgtgcc gccagcgcga cgtcgatctc
    16561 catatcgtca tgagtcgaaa cgcccagccg caggcccgct gcacggatgg cattgagatc
    16621 ggtggcttgc aaatcttcct gccccagatg gacgccatac gcctgatgct taatcgccag
    16681 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16741 caccacatcg gcttctacct cttcatcgcg ctgatctttg atgcgtagct ggagagtacg
    16801 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tcaaccaccg ggtacagtcc
    16861 taaacgaaag ggtacaggag gaaactcagg ctgatacatc acgcttcctc cttacgcagg
    16921 tagatttctc cgcctctggc gcggaagttc tccgacatat ccgccattcc cacttcaatg
    16981 gtttgcgcgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggt
    17041 ccacacatgg agcaaaaatg ggcgacttta ccggactctt gcggcagggt ttcatcgtga
    17101 taagcgcggg cagtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    17161 aagcgggctt tcgacatggc gttatcgcga atttgcgcgc ccggatgccc tttcgccagg
    17221 tcggcggcgt gagcggcaat cttataggtg ataagcccct gtttaacatc ttctttattt
    17281 ggcaggccca gatgctcttt cggcgttacg tagcagagca tcgcgcagcc aaaccagcca
    17341 atcatcgccg cgccaatccc tgacgtgaag tggtcatagc ccggcgcgat atcggtagtt
    17401 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    17461 cggcgaatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacgtcatat
    17521 tcccaggcga ttttggtcag ttcgcccagc gtatgcagct cggcaaactg tgcttcatcg
    17581 ttggcgtcct gaatagaacc ggggcgcagg ccgtcgccca gcgacagcga aacgtcataa
    17641 gcagcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    17701 gagaggcacc atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc
    17761 gcggtcatcg gcacgtagcg cagcagcacc cccgcatgga tagtgaagta atccacacct
    17821 tgctcggctt gttccagcag ggtgtcgcgg aagacttccc aggtaagatc ttcagcgatc
    17881 ccgttcacct tctccagtgc ctggtagatc ggcactgtac cgatcggcac cgggctgtta
    17941 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccag tggaaagatc catcacggta
    18001 tccgctcccc agcgcgtgga ccataccagt ttttccactt cttcttcgat ggaagaagtg
    18061 accgccgagt tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc
    18121 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    18181 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    18241 tgctggtggc gtaaaacttc gctacgtatg cgctcgcgac ccatattctc gcgaatggcg
    18301 atgaattcca tttctggcgt gatgataccc ttgcgggcgt agtgcagttg ggtgacgcgg
    18361 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcaaggcca
    18421 tcatctgcca gccgcgcttt agtgtaatcg gaactgcgta cggtaagttc ttcggtatcg
    18481 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggca
    18541 atctgcggat cgccatacgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    18601 tactgcggct gttctttgct gccgccaatt agcgtcgggc taagctggat ctcacgcatc
    18661 ggcacgcgca cgccggggtg tgtgccagtg atgtaaatgc gttttgagtt gggaaaggcg
    18721 gagccttcca gggtgtcgat aaaatgttgg gcctgggcgc gctgttcgcg gcgggtcagt
    18781 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18841 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18901 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18961 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    19021 cagaactcaa gcaggatatt tgacgcgggc ggcgtcaagc accagtagaa tcagcttatc
    19081 ttccagcaca aagcgcgctt ccagtgcctc gccgatgtca gataaaacct gttgaaactc
    19141 aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtcat ccataatctg
    19201 ttgagtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc
    19261 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    19321 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    19381 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    19441 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    19501 catgattgac tccgcaagtt tgtattcaaa aactgctctg tgagaaatgt aaaaaccatg
    19561 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    19621 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    19681 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19741 atggcagggg gaacctgttt ggttagtaca acagcagcgg cgtcacgata tggggtcggt
    19801 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtgcaact
    19861 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19921 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19981 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    20041 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggtg aaaccctcga
    20101 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
    20161 cgtgacctcc caaccgtggc cgtttcctca gtcgttaatg accgcgttta tggcggaata
    20221 tgacagcggc gagatcgtga tcgacccgaa agaattgctt gaggcgaact ggtatcgcta
    20281 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    20341 ggtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    20401 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    20461 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    20521 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaatgccg agctggcgtg
    20581 cgaagtgact ttgcaaccgc tgcgtcgcta tccgctggat gcggcgatcc tcttttccga
    20641 tatcctcacc gtgccagacg cgatgggttt agggctctat tttgaagccg gagaaggtcc
    20701 gcgttttacc tcgccagtca cctgcaaagc cgatgtcgat aaactgccaa ttccggaccc
    20761 ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20821 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20881 aggcggcagc agcaaagcgt tcaccgtgat caaaaaaatg atgtatgccg atccacaggc
    20941 gctgcacgcg ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat
    21001 taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    21061 cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt tactgcgtga
    21121 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggtggcggac agtggctgga
    21181 agcgatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    21241 tgcgcgccgt cgcgtgggca ataaagtcgc gctgcagggt aatatggacc cgtcgatgct
    21301 gtatgcgccg cctgcccgca ttgaagaaga agtggcgtct atacttgccg gtttcggtca
    21361 cggcgaaggc catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    21421 tgctggcgtg tttgtggagg cagtgcatcg cttgtctgaa cagtatcacc gctaaggagc
    21481 gattatggat ctcgcgtcat tacgcgctca acaaattgaa ctggcttctt ctgtgatccg
    21541 cgaggatcga ctcgataaag atccgccgga tctgatcgcc ggagccgatg tcggatttga
    21601 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    21661 ggtggagtat aaagtggccc gcatcgccac caccatgcct tacattccag gttttctctc
    21721 gttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt
    21781 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtag ccagccattt
    21841 tggcttattg gtggatgtgc cgaccattgg cgtggcgaaa aaacggctct gcggtaaatt
    21901 cgaaccgctc tccagcgaac cgggcgcgct ggcccctctg atggataaag gcgagcagct
    21961 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
    22021 ggtcagcgtg gacagcgcgc tggagtgggt acaacgctgc atgaaaggct atcgtctgcc
    22081 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    22141 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    22201 gttacaaaac ccaattcatc tgcgtctgga gcgcctggca agctggcagc atgtcacttt
    22261 tatggcctgc ttatgcgaac gcatgtaccc caattacgcc gtattctgtc agcaaaccgg
    22321 cttcggcgat gggcagattt accgccgcat tctcgatctc atttgggaaa cgctgaccgt
    22381 caaagatgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    22441 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcttgcg tggcgttaag
    22501 tgaactggtt cattcgcgtt taagtggcga aacgctcgaa cacgcggtgg aagtgagtaa
    22561 gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    22621 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    22681 ccgactttta gctgagtgtg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22741 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22801 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22861 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22921 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22981 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    23041 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    23101 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    23161 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    23221 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    23281 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggcag gtgcgctgtt
    23341 tctcactgcc tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    23401 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    23461 cgtgtttagc ccgtggcgca gtggcgatac gacgacgcga gagtatcgct ggcagggcga
    23521 taacctcacg cttatcaata tcaacgttta cagcaaaccg ccggtaaaca tccgggcgcg
    23581 ttttgacgat cgcggtgact tgagctttat gcaacgtgaa tccgacgggc aaaagcagca
    23641 gctttccaat gatcagatcg atttataccg ttatcgtgcc gcgcagatcc gcgagattag
    23701 cgatgcctta cgccagggga gagtcgtgct gcgccagggg cgctggcatg cgatggagca
    23761 gactgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
    23821 gcatatcgag cgccgccaga gccgttcttc tgttgatgtc agcgtggcat ggctggaagc
    23881 gcccgaaggt tcgcaattac tgttggtggc aaactctgat ttctgtcgct ggcaacccaa
    23941 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    24001 ccgtaaccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    24061 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    24121 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg ctttgtcacc
    24181 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    24241 gtttgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    24301 gcaaatgccg atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    24361 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    24421 tcgtgccaac ggcgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    24481 gactcgcctg ttgtgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    24541 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgggc
    24601 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    24661 gcgcgatcct ccccgtggtc attgttgggc tggtggggct gtttgcggtg acagtgattc
    24721 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24781 ttatccgtgc tctcgaatcc ggctcgcgcg tcggaatggg gatgcgcatg catcatgcgc
    24841 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24901 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24961 attccccgca ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg
    25021 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    25081 ctggaatgca ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttta
    25141 tcgctttcga cgccagtaac attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    25201 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt gttgtcattc ttctggtatc
    25261 gccgatatct gcgctcgggc cagctgttgc aggatgaaat gaagcgcaaa gagaagctgg
    25321 tggcactggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga
    25381 ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    25441 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagctgctgg
    25501 aactggttaa gccaacgcat ctggctttgc aggcggtgga gctcaacacg ctgattagcc
    25561 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    25621 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    25681 atctctatct caacgctatt caggcgattg gtctgcatgg cgtgattagc gtgacggcca
    25741 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcgg
    25801 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggactgg
    25861 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gttgcaagcc
    25921 aggaggggaa aggcgcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    25981 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    26041 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    26101 gcgacaggcg ctggagcagg tgcgggaaca ggttttcgat cttgtgcttt gcgatgtgcg
    26161 aatggcagag atggacggca tcgccacgct aaaagagatc aaagcgttaa atccggcgat
    26221 tccggtactg attatgaccg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    26281 cggggcgctg gattacctca tcaagccgct ggatttcgat aacctgcagg caacgctgga
    26341 aaaggcgctc gcgcatacgc acagtatcga cgctgaaacg cctgcggtgt ctgccagcca
    26401 gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    26461 cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggtaccggta aagagctggt
    26521 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    26581 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcatgaaa aaggggcgtt
    26641 taccggagcc gacaaacggc gagaagggcg ctttgttgag gcggacggcg gcacgctgtt
    26701 tctcgatgag attggcgata tctcgccgat gatgcaggtt cgtctgctgc gtgcgattca
    26761 ggagcgcgaa gtgcaacgtg tcggcagcaa ccagactatc tcggttgatg tccggctgat
    26821 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26881 ctactatcgg ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    26941 tattcctctg ctggctggcc attttcttca gcgctttgcc gaacgtaatc gcaaggcggt
    27001 aaaaggcttt acgccccagg cgatggatct gctgattcat tacgactggc cgggaaatat
    27061 tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc
    27121 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggtac aaagtcagga
    27181 tattcagccg ctggtggaag tcgaaaaaga ggtgattctg gcggcgctgg agaaaacggg
    27241 cggcaacaaa accgaagccg cccgccagtt agggatcacg cgcaaaacgc tattggcaaa
    27301 actgtcgcgt tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa
    27361 gcagtcgtcc caatggatat cggtcattaa ggcataagcg cgtttctgcg cttctgccac
    27421 ggtatgaccc agcgcggtaa cgcacagtac gcgcccgccg ctggtcacta cctgctcgtc
    27481 atccgccagt ttcgtgcccg cgtggaacac tttgccgtct gccacttctt ccagcggcag
    27541 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    27601 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    27661 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27721 atcgccaaag cggcagttaa attcaataac ctttggatta ccctgtttgt cgatcatcag
    27781 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27841 ccagatgata cgttccatgg tgcgctgatg aacttcatcg gtcactaccg gcgcaggaga
    27901 gtaagccccc attccgccgg tgtttggccc ggtatcttta tcgcctacgc gtttgtgatc
    27961 ctggctggtg gccatcggta gcacatgctc gccgtccacc atcacgataa agctcgcttc
    28021 ttcaccgtcg aggaactctt cgataacaat acgatgaccc gcgtcgccaa acgcgttgcc
    28081 cgccagcata tcgtgaacag ctgcttcagc ttcttccagc gtcatcgcca cgataacgcc
    28141 tttcccggct gccagaccgt cagctttaat gacgattggc gcgcctttct cacgcagata
    28201 tgccagcgca ggttctacct cggtgaagtt ctggtattcc gctgtgggaa tgttatggcg
    28261 ggccagaaag tctttggtga atgctttaga accttccagt tgtgcagcac ctgcggttgg
    28321 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgact acgcctttca ccagcggcgc
    28381 ttccgggccg acgatggtta gatctacctt ttcgttttgt gcgaaatcca gcagcgccgg
    28441 gatatcggtt acgccgatag cgacgttctg tagcgcgggt tccagcgcag tgcctgcatt
    28501 gcccggagca acaaaaacag tttcaaccag cggcgactga gccgctttcc aggccagcgc
    28561 gtgctcgcgc ccgccgttac cgatcactaa tactttcatc tgtcgctcca ttaatggcgg
    28621 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaattact
    28681 tcgtcatcac ggatagaacc gccaggctgg attacgcagg tcacgcccgc agcggctgcg
    28741 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccattgacga acctttcact
    28801 tccaaacctt catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    28861 tggcctgcgc caataccgat ggtcatattg tttttggcat agacgatagc gttggatttc
    28921 acgaacttcg ccactttcca gcagaacagc gcatcacgaa gttcctgttc agtcggctga
    28981 cgtttggtga cgacgcgcag ttcttccgca ccgaccatcc ccagatcgcg atcctgaacc
    29041 agtagaccgc cgttcacgcg tttgaaatca agacccggaa tacgttcgcc ccactgaccg
    29101 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    29161 gacggcgcaa taatcacttc gacgaactga cgagaaatga tggcctgcgc ggtttcagca
    29221 tccagttcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    29281 gcgcgatcgt aagcatcaag aatggaattg ccgatagcca cgccgcaagg gttggcgtgc
    29341 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    29401 tcggcgatgt tgttataaga gagagcttta ccctgaacct gggttgcggt agcaacggag
    29461 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    29521 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct
    29581 tctttgcttt caccgtggta agccggaacc atgctgccga agtagttggc aatcatgctg
    29641 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    29701 agcgatcctt cgttggcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    29761 acgattgcga catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata
    29821 tcgatgttct ctaccgcatc ttccagcgag cagccttcac gagcgacggt ctgggcgaac
    29881 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29941 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    30001 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacat
    30061 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    30121 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    30181 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    30241 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    30301 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    30361 attttgacag tggctaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    30421 ctgtgtgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    30481 ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    30541 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc
    30601 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag
    30661 ccgcgtcgca actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac
    30721 gg
//
LOCUS       DAPQZD010000046        30671 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPQZD010000046 DAPQZD010000000
VERSION     DAPQZD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.046"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(77..1399)
                     /gene="hipA"
                     /locus_tag="RO454_001993"
     CDS             complement(77..1399)
                     /gene="hipA"
                     /locus_tag="RO454_001993"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDY0933833.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK"
     gene            complement(1399..1665)
                     /gene="hipB"
                     /locus_tag="RO454_001994"
     CDS             complement(1399..1665)
                     /gene="hipB"
                     /locus_tag="RO454_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDY0933834.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            1973..2731
                     /gene="tam"
                     /locus_tag="RO454_001995"
     CDS             1973..2731
                     /gene="tam"
                     /locus_tag="RO454_001995"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDY0933835.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(2735..3649)
                     /locus_tag="RO454_001996"
     CDS             complement(2735..3649)
                     /locus_tag="RO454_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDY0933836.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(3846..5297)
                     /gene="uxaB"
                     /locus_tag="RO454_001997"
     CDS             complement(3846..5297)
                     /gene="uxaB"
                     /locus_tag="RO454_001997"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDY0933837.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5524..6942)
                     /locus_tag="RO454_001998"
     CDS             complement(5524..6942)
                     /locus_tag="RO454_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDY0933838.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7081..7440)
                     /locus_tag="RO454_001999"
     CDS             complement(7081..7440)
                     /locus_tag="RO454_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDY0933839.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(7440..8366)
                     /gene="glsB"
                     /locus_tag="RO454_002000"
     CDS             complement(7440..8366)
                     /gene="glsB"
                     /locus_tag="RO454_002000"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDY0933840.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8430..9818)
                     /gene="sad"
                     /locus_tag="RO454_002001"
     CDS             complement(8430..9818)
                     /gene="sad"
                     /locus_tag="RO454_002001"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDY0933841.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            9919..10800
                     /locus_tag="RO454_002002"
     CDS             9919..10800
                     /locus_tag="RO454_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0933842.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            10878..10961
                     /gene="yneK"
                     /locus_tag="RO454_002003"
                     /pseudo
     CDS             10878..10961
                     /gene="yneK"
                     /locus_tag="RO454_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11139..12329
                     /gene="ydeA"
                     /locus_tag="RO454_002004"
     CDS             11139..12329
                     /gene="ydeA"
                     /locus_tag="RO454_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDY0933843.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12354..13019)
                     /gene="marC"
                     /locus_tag="RO454_002005"
     CDS             complement(12354..13019)
                     /gene="marC"
                     /locus_tag="RO454_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDY0933844.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13231..13665
                     /gene="marR"
                     /locus_tag="RO454_002006"
     CDS             13231..13665
                     /gene="marR"
                     /locus_tag="RO454_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDY0933845.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            13686..14069
                     /gene="marA"
                     /locus_tag="RO454_002007"
     CDS             13686..14069
                     /gene="marA"
                     /locus_tag="RO454_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDY0933846.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14101..14319
                     /gene="marB"
                     /locus_tag="RO454_002008"
     CDS             14101..14319
                     /gene="marB"
                     /locus_tag="RO454_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDY0933847.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14350..15249)
                     /gene="eamA"
                     /locus_tag="RO454_002009"
     CDS             complement(14350..15249)
                     /gene="eamA"
                     /locus_tag="RO454_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDY0933848.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            15444..16631
                     /gene="ydeE"
                     /locus_tag="RO454_002010"
     CDS             15444..16631
                     /gene="ydeE"
                     /locus_tag="RO454_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDY0933849.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            16758..16853
                     /gene="mgtS"
                     /locus_tag="RO454_002011"
     CDS             16758..16853
                     /gene="mgtS"
                     /locus_tag="RO454_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDY0933850.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17072..17962)
                     /gene="dgcZ"
                     /locus_tag="RO454_002012"
     CDS             complement(17072..17962)
                     /gene="dgcZ"
                     /locus_tag="RO454_002012"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDY0933851.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(18216..18320)
                     /locus_tag="RO454_002013"
                     /pseudo
     CDS             complement(18216..18320)
                     /locus_tag="RO454_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
     gene            complement(18469..20514)
                     /gene="dcp"
                     /locus_tag="RO454_002014"
     CDS             complement(18469..20514)
                     /gene="dcp"
                     /locus_tag="RO454_002014"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDY0933852.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            20651..21397
                     /gene="ydfG"
                     /locus_tag="RO454_002015"
     CDS             20651..21397
                     /gene="ydfG"
                     /locus_tag="RO454_002015"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDY0933853.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            21486..22172
                     /gene="ydfH"
                     /locus_tag="RO454_002016"
     CDS             21486..22172
                     /gene="ydfH"
                     /locus_tag="RO454_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDY0933854.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            22349..22552
                     /gene="ydfZ"
                     /locus_tag="RO454_002017"
     CDS             22349..22552
                     /gene="ydfZ"
                     /locus_tag="RO454_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDY0933855.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(22588..24048)
                     /locus_tag="RO454_002018"
     CDS             complement(22588..24048)
                     /locus_tag="RO454_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDY0933856.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(24137..25504)
                     /gene="ydfJ"
                     /locus_tag="RO454_002019"
     CDS             complement(24137..25504)
                     /gene="ydfJ"
                     /locus_tag="RO454_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDY0933857.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALQDAAEDQPHVVRAAQ
                     PSRSL"
     gene            complement(25562..26581)
                     /locus_tag="RO454_002020"
     CDS             complement(25562..26581)
                     /locus_tag="RO454_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDY0933858.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(26593..27807)
                     /gene="rspA"
                     /locus_tag="RO454_002021"
     CDS             complement(26593..27807)
                     /gene="rspA"
                     /locus_tag="RO454_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDY0933859.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(28013..28339)
                     /locus_tag="RO454_002022"
     CDS             complement(28013..28339)
                     /locus_tag="RO454_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDY0933860.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            28474..28815
                     /locus_tag="RO454_002023"
     CDS             28474..28815
                     /locus_tag="RO454_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDY0933861.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            28850..29410
                     /gene="speG"
                     /locus_tag="RO454_002024"
     CDS             28850..29410
                     /gene="speG"
                     /locus_tag="RO454_002024"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDY0933862.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(29413..30123)
                     /locus_tag="RO454_002025"
     CDS             complement(29413..30123)
                     /locus_tag="RO454_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDY0933863.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            30231..30536
                     /locus_tag="RO454_002026"
     CDS             30231..30536
                     /locus_tag="RO454_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDY0933864.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         8065 a         7796 c         7022 g         7788 t
ORIGIN      
        1 tataaaccaa gggcgaatcc agtcgttggc ggttcatgat tgttctgctc attaacaacg
       61 accaaaaccc catatcttac ttaataccgt attctcggct taaccgatcg tgcaacctca
      121 acacattggt ttcaactgcc gtcaccacgt tctccggaaa atctgtcggt aatgaagtct
      181 tcacgttatc cagtgctgcc ggaatcattc tggcaaagtc actcaggatt tcatgcatct
      241 gcacttccgg gaatttcagc acctttgctg tcgccagaaa atgtcgcgga taaattttat
      301 cgattgccgt ttttttgcct ttggatgcgt taagccccat tgccagtttg agatcgctga
      361 tgtgtattcc cgtaccgcca aggaccggaa atgccgaaat gatgtcgtaa aatggtgtga
      421 gccgataact gccgccagcc tgaataaata cggagaagtt ttttgcatgg ccatccgttg
      481 cgccaatcaa ccactggaag acctgaaact tcataaaatc atagcgatct ttcagcgcct
      541 cgctggaccc catcaaaaaa gccataattt gcgcgatacc ggggcctcca tctgattcat
      601 atttcaccga tgaaggtaaa ccgaatgtct ggcacatatc ctcctgtggc aagcgaagta
      661 aaaccgttcg ctcagtattc caacgcctgt caaaacgttc gaccgctaac gcgcgcaccc
      721 ttcccgcttt aatgatttct gcgtccggaa cattcaaccc gagttctttc gccagcagca
      781 aacagtaata ctcattatca acgctttggc taagatcgag cgtcgcattg ggctgtctga
      841 tttcgccaat cggcaatttg ataatgtgcg tcgttggcgt tattcccttc ggaatgcacc
      901 agtcattgcc tattctgagc agcgcagtct tctcctgtgc gccagcaacc gagatgcgaa
      961 agtcattttc ttctctaatc atgcctaacg ggatatctgc tttataagcc gttaatactt
     1021 cttcaagtct ggcttcagta agcttttccc atgccattat cggacacgtt acggtttcat
     1081 cttcggggat taacgtcacg gcaccaacgc tgtctcgccc tatttctgac aataaatcaa
     1141 acggttgtct ggatttagca tgataacgtt taacgatccg gtcacgtaca atcgggctat
     1201 cgggtaacag gttatcgaag aagttaaata cggcatcaga ggtgatattc cccctctgca
     1261 atggcagcga aagtgacaac ggtctggcat aacggctggc taaccactca gctgcatact
     1321 taaaggtgtg cgcgccgttg gctaacttcg ttaactcgcc tacccgctgg ttgttcatcc
     1381 aagtgactag tttaggcatt accactccag atcttgctgt tctgttgctt ctggcgaggc
     1441 atttttcgcg tcgcatagcg tcattgagag ttcaagcgac tgtaaaatct taaaaaatgt
     1501 cgtgagcgag gtattgtcag gattgttttc gaaattggaa attgtcgcct gcttaatgcc
     1561 gatttttttc gccaactcgc tctgcgtcca gccgttttgc tggcgaacca gtttcattgc
     1621 attcgccaat tgcgttgggc tatagatctt ctgaaagctc atcatgtcat acgtccacag
     1681 caaatttatc cgcttaaggg gatattatca gttttatcct ttagtgagga taaagtcaat
     1741 ctggaaacac atcttatccg ctctacggga taaacgctgc gatatccgcg atcgcggata
     1801 aaaaggagga tttaaaaaag cacatcctaa tgcggtggct tctttgacta ccggaatctg
     1861 taatcttaat gaccgggccg cgtaaaaaac gtctgttcaa tggggtgatg ccgtgaggcg
     1921 aatttatcag ttttatctac aattggggta acgcgctgac gggagtaaaa aaatgtctga
     1981 ctggaacccc tctttatatc tgcactttgc cgctgaacga tcgcgtccgg cggtggagct
     2041 gcttgccagg gtgccgctgg agaatatcga atacattgcc gatcttggct gtggcccagg
     2101 taacatcacc gcccttctac accaacgttg gcctgctgcc aggataacgg gcatcgactc
     2161 atctccggcg atgattgctg aagcgcgcag tgctttgcca gactgcctgt ttgtggaagc
     2221 agatatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     2281 gcaatggctg cccgaccact acgaattgtt tcctcatttg gtttcgttac ttaattcgca
     2341 tggtgtgttg gcaatacaga tgccagataa ctggctagag ccaacccatg tgctcatgcg
     2401 cgaagtcgcc tgggaacaaa attatccgga tcgcgggcgt gagccgctgg ctggcgttca
     2461 tgcttactac gatattctga gtgaagccgg atgtgaggtc gatatctggc gaaccactta
     2521 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     2581 tccgtggtta caggatctga ccgagagcga acagcagcat tttcttacgc gctaccatca
     2641 gatgctggaa gagcaatatc ccctccagga gaacggacag atattgctgg catttccgcg
     2701 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tggcgatcgg
     2761 gaagaatttt cgctggaatt tctgcttcat cattcatctg taacaggtca atttcaatag
     2821 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaaggga tcttccagtt
     2881 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2941 tcatgtaatg caggtccacg accagcgcaa acggcaacat gatacaaaac agatacacgg
     3001 tgcgatgcaa aatcagggtg taggcaaagg gaattggcgt ataggcaatg cgctcgcatc
     3061 ctgccaggac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     3121 aaagttgtcc attgcgtcgc tgaaccgcca accactcccc cattattaac aagatgcggt
     3181 tagctggaga gttagaagcc agtacacgct gaagatcttc agtcttgaga taatgagcca
     3241 gcacttctgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aagacgattt
     3301 gcagccgggc aaactccctt acacttgccg aatccggtaa tgtcgttttt acctcgcgca
     3361 gtaacgaccg cgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     3421 cgtaaccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     3481 acggtgcgag ggtgaattta atgcccaaat gcgtgtacca gggcagcatg aaaataacag
     3541 cgatcgaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgaaccgt
     3601 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     3661 ggcgtggaaa agtcgcccta ttaaacgtga ttatgatcac attctcaaga tactacttac
     3721 aaattaccta caagattaca acattaacag gtgctgcatg tggcacctgt taatgataaa
     3781 ggcatataga atgttgtaac taatatagcg gaataagaaa cccggtcgaa accgggttca
     3841 gagtattagc acaacgggcg tacagcttcg cgcatccctt tttcgagaat cgcatccagg
     3901 tcattagcaa cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct
     3961 ttttctgcca acacaatcgc taccagttcc tgggtcccga taacacggtc acgatactgg
     4021 ctccagagtt gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct
     4081 ccattacgca caccgcgata gaacgcaatc aatgccgcta atgcgaaagt aaggcgcgcc
     4141 ggaagtgtgc cgtttgcctt ctgccctgcc agcagctgcg gcaggatgcg tgtgcggaac
     4201 ttggtcatac cgttgagcgc gatagacagc agttgatgct tgatgtacgg gttacggaaa
     4261 cgtccggtga ctgcactggc gaaagactcc agctcatcgc gtggcaaatc cagtaccgga
     4321 ataatttctt cgtagatggc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct
     4381 tcacctacgg tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg
     4441 ttgaggattg ccactttgcg ttctttatac ggtttaatat cgtcaacgat cagcacgtta
     4501 agcggatatt tgtccagacg aagttcagtc gctaaggatt tcggcccctg aatcacaaac
     4561 aggtaaaagt gttcagcggt gtcgaggaaa ccatcgtggt agcccagttc ttcttccagt
     4621 ttagccactt catcacgcgg ataaccggta acgatacggt ccaccagcgt agaacagaag
     4681 ctgttagcct gatccagcca ttgaataaat gcttcaggca atgcccactc ttgcgcatag
     4741 cgcagcacca gttcacgcaa cgcgtcgcca ttatagtcaa tcaactcaca cgggatgatg
     4801 atccaacctt tatccagcgc accgttgaaa tggctaaaac gttcgaacag cagacgggtc
     4861 agttttgccg gataacttac cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg
     4921 atacccgctt cggtggtgtt agagaaaaca aagcgcattt ccgggttgtg agccagtttc
     4981 aggaattcat cgtattcact atagacgctg atttcacgat taaccgagcg aatcagacgc
     5041 gcgtcgctga ccgcttcacc cttctcattc aggccacgga taatggtggt atacagacca
     5101 tcctgcgtgc tcagtgacgg cgggaatgaa gtttcaatcg gacgaacaac gaccacgcca
     5161 gaattcagat cggtgtgctc attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg
     5221 aagttacctt caccaaactg aatgatacgt tctggatact gtgcaccggg aaaatcgcga
     5281 cgatttagtg ttttcacaat gggttccctt ctgattagtc atacaacctg tttgaattgg
     5341 tacgacaggt tagcaaactt taatacgtcg aacccctgtt ttgatcaact cctgatgatt
     5401 aatgagcagt tttatgagaa aagtgtggcg cggatcatgg tttaatcgag gaaacaggcc
     5461 ttttcttgga tcataaagtg gtagaacaca ttgcatttaa ttcgctctga gtaaataaag
     5521 atgtcagaca acttcctcac cgtaacgcat agtgctggta cggttgcgcc catctttctt
     5581 cgagcgatac agacacgtat cggcttcgac catcaattta ttaaagtcat cggtcagcgt
     5641 tcggtacgat gcgcaaccac taccgacgcc aatacttacc gtgagataaa gcgttttttg
     5701 ttgccaggtg aacggttgca gttcaacgcc tttacggatt ttttccgcca ttagcaggcc
     5761 atcgacagga ttcacagacg gcactgcaac agcaaattct tcgccgccca ttcgcgccac
     5821 cagcccctta tcaccgacaa tcttctgaat atgctgggca aacacgctta acactttatc
     5881 gccacattca tgtccatagt tatcgttaat gcttttgaaa tagtcgatat caagcagcat
     5941 gaccgtcaga tgttgtgtct gtttcagcga tggacttttc agcgcctcat aaagaccgga
     6001 ccgtgagtaa acctgggtca gaaaatcaaa atcggctcgc agcgcaactt gcttcattag
     6061 cgaattgatc gctgccacgc taaaggaaac cataattggg catatcgcca tcgtggcaat
     6121 accgagacgt gcggagaaca tttgcggaat ggagaacggc gaaccgaccg agatatcaat
     6181 caccgaattt gccaccagta cgacttccac agcgccggtg acaaaggtca acagacatgt
     6241 tacttgcggc gtatagcgca ctgcacacca gattaatgca gggagcggaa acgccagact
     6301 ccccgctccg ccaatgacta ccgaggcaat aaccgacaca ataagcgcga tagcaggcat
     6361 catctgctct gctttaaagc gcggcagcac tccaggaatt gccaacgtca gcatacaagg
     6421 cacgatcaac acgcccgttg agaattgctc actaaaccag tcggcaagca aaggccagaa
     6481 atccagagta tcaatactga ccgaaccaat cgccccgaca atagcgcata ataatgcggc
     6541 aagcagacag taattaaata accgtaaggc acttactggc tcatacttat tttttcccag
     6601 acgcttatcc cgcgcgacca gtaaggcgac ggtaacaata aacatcatat tggagaaatt
     6661 gatagccagt gaaaccagcc cccattcggt ggttatggca tcataaacca gcatcgccac
     6721 ataactgatc gcatagtaat gcagtcgatt aagccagaca tagcgggcaa acacccccgc
     6781 cattacgcca ttcagcggcc agaaaagaga aagttcgtga accagccgta actctgcacc
     6841 aataaaatag aacagcgtgg ttagcacaaa aatagcgatg ctattacgta gcagatgacc
     6901 ttcctggaac aaacggaaag ttgaaatggg ctgtacatgc attaattatc tgtccataca
     6961 attgcgggtg gcaattatct caacgcaata agtaaattct tagaaaggat agcatgttcg
     7021 ctgacaacgg cggcaactaa agatacctaa tgaatgccat tttatcgatt tccgtaatta
     7081 tcatggctga ttcatctgta taaccagcca gtgatagatt accgtcacaa tataacgttg
     7141 ttgctcctca cttaacgctt ccccctgagg aatgttatgc cacttagcca gacaaccgcg
     7201 acaacaggtc gcggtggcgt gttgagcgat aaaaaccgga tgcccgcgca tgggggtttg
     7261 cttaccgtcg ttagccggta atgccggagc aagacgttta gcgacaaaat ctgccgcatg
     7321 ttgttcaatg actggcgcgc ctttctccag acaatactga cgctctttca cacccaaacg
     7381 aaagcgagag cgaaattttg aacgggataa acgcgcgaag agtggatcga gagactgcat
     7441 taataaactg aacgccctaa ttgtttcgtc aattgttcaa ggacggcaat gcccgcaagc
     7501 gagttaccgg catcatccag ttccggactc cagacagcga tggccatttc atgcggaaca
     7561 atcgccacaa taccgccacc aacgccagat tttgccggta gtcccacccg ccaggcaaac
     7621 tcccccgcgt tctggtacat accgctggtc gccatcagcg cgttaatttg ccgcgcctgc
     7681 attggcgtca ccacaggttc atcaatatga atagctttcc cctgattcgc cagaaagaca
     7741 aacgtccggg ccagctctac acagctcatt ttcagagcgc agtaatgaaa gtagttttgc
     7801 agaacggttg tcacgtcatg atggaaattg ccaaacgact tcatcagcca ggcgatagcc
     7861 gcatttcgcg cggaatgttc aaattcggaa cgcgctacca ccgtatcgta ggaaatatca
     7921 gacacaccac ttaagccgcg cacgacttcc agcatacgtt gccgaggtgc gcttaatcgc
     7981 ccttgcaaca tatcgcagac caccagcgca ccggcattaa taaacggatt acgcggaata
     8041 ccctgctcca tttccagttg cactaaagaa ttgaatggtg atccagacgg atctttgccg
     8101 acgcgttgcc agatttcctc ttcggagtaa tgacgcatgg cgacaacgag actcagcact
     8161 ttggaaatag actgaatgga aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg
     8221 tcaacggtac agatagcaat ccccaatcgg gaaccgtcta ccgtagccag cgccggaata
     8281 taatccgcga ctttaccctg accaatgagc ggccgcactt gccgcaagat gttttctaaa
     8341 attgcattat ccatggcgac tgccactttc tactcctgga ccgcaggtct gaaaagacct
     8401 gcgagtatat cagagctgaa tatatagcgt cagacccggt ctttccacac cgtctggata
     8461 ttacagaatt cgtgtaagcc gaaatgggaa agctcacgac caaagccact cttcttcacg
     8521 ccaccaaagg cgactcgcgc atcgctggca caataaccat tgataaacac cccaccgcat
     8581 tccagacgtg ccgccatctg tctggcctgt gtttcgtcgg tggtgaaaat ggtcgctgaa
     8641 aggccgaact cactatcatt agccagttcc agcgcatgtt ctgcatcttt cgcaatggtg
     8701 attgccgcaa cggggccaaa catttcttcc cgaaacgcgg tcatttctgg ggtaacattc
     8761 gccagaaccg ttggcggata gtagttacct gccccagcca tcttttcccc gcccagtaac
     8821 aaacgcgcac cctgcgccag tgttttttcc acctgatgat gcagctcatc acgtaaatca
     8881 aaacgagcca ttggtccgag agcgttgtct tcatcacggg gatcgcccat tttcaaggct
     8941 gccgcagctg ccacaaaacg ttcggtaaat gccgaagcaa ttccctcttc gatgataaag
     9001 cgttttgccg ctgcacaaac ctgtccggta ttctgataac gtccggctac cgccgctttc
     9061 accgccagtt ccagatcggc atcgttaagc acaataaacg gatccgaacc acccagttcc
     9121 agtacgcatt ttttcagtgc cgctccagcc tgtgcgccaa tagccgctcc agcacgaaca
     9181 cttccggtca ctgtgacagc agcaatgcgc gaatctttaa tcatctgact gacaccgtcg
     9241 ttgtcggcat tcagccagcc atatacgcct tgtgggatac ccgcatcttt aaacacctgg
     9301 gcaacgagct gtgcacagcc catcacattc ggcgcatgtt taagtaagta gccgttacca
     9361 gcaagaatga tgggaacagc gccacgcatc acctgccata acggaaaatt ccacggcata
     9421 atcgccagaa tcgtccccaa cggtcgatac tcaataaccg cctgctgatt ttccaccagc
     9481 gtaggttctg ccttcagcat tgccggacca tgttctgcat accagtcaca caaattcgcc
     9541 gatttcgcca cttcagcgcg cgcctggttg attggtttgc ccatttcacg ggtgatcatt
     9601 tgcgccattt cttcgctacg ggcgcgcaga gccttaccga tatcacgcag tttttcagca
     9661 cgataatcta tatttgtctc gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg
     9721 ttttcgatat cgtcaacacc agcccacggc agcacagaaa gttgttcacc ggtggcagga
     9781 tttatcgaaa ttgcatgagt tgccggagta atggtcatcg gggtatctcc tttatgagtc
     9841 atggtatgaa gatacgcaga tttactcttg ctttaaaatg aataatatta agccacttat
     9901 tcacgaatcg agaatgccat ggatctgacc caactggaga tgttcaacgc ggttgccgaa
     9961 gctggcagca ttacccaggc tgcagcaaaa gtgcatcgcg tgccgtcgaa tctcactacc
    10021 cgtctacgtc agctggaaac agaactgggg gttgatctgt ttattcgcga gaatcagcgt
    10081 ttacgtctct cgcctgccgg gcataacttt ttacgctata gccaacaaat tctcgcatta
    10141 gtggatgaag cgcggagcgt tgtcgctggc gatgagccgc aaggtttatt ttctctggga
    10201 tcgctggaaa gcaccgctgc agtgcgcatt ccggccacgc tggcggaatt taaccgtcgt
    10261 tatcccaaaa ttcagttttc actttccacc ggcccttccg gcactatgct ggaaggtgtg
    10321 ctggagggaa aactgaatgc ggcgtttatt gatggaccca ttaaccatac tgccatcgac
    10381 gggataccgg tataccgcga ggaactgatg atcgtcacgc cacaaggaca tgcgccagta
    10441 acccgtgcca gtcaggtgaa tggcagtaac atttatgcct tccgcgccaa ttgttcgtat
    10501 cgtcgccact tcgaaagctg gtttcatgct gacggtgccg ctccgggaac tattcacgag
    10561 atggagtctt atcacgggat gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt
    10621 ccacgctcta tgctggaaag tatgccgggg catcaccagg ttgaatcgtg gccgttagct
    10681 gagcaatggc gttggttaac aacctggctg gtctggcgtc gtggtgcgaa aacgcgtccg
    10741 cttgaggcat ttattcaact gctggatgcg cctgactcgg caaaacaggg ttaccaatga
    10801 gctatttttg atagttcatg cggctaatat gctctatata gtgatgttcc aatgacttat
    10861 gactatatgg ggcaaatatg gttacgccag taagcatcag caattacata tctcttcctg
    10921 atgattttcc cgttcgtaat attgcacctc aagtaaaaga agaacaggcc cccttcctga
    10981 tcgtcattaa aatgtgatgt taacaactct ctgatgcgtg acagaacaac cgtcagagag
    11041 atggaaatgc gatccagatc acaaatgcat tgtattcaca tcattaaccg ttttaagatc
    11101 atttcatcac tttttcgcaa ctcacccgat aatctgttat gacaacaaac actgtttccc
    11161 gcaaagtagc gtggctacgg gtcgttacgc tggcagtcgc cgcctttatc ttcaatacca
    11221 ccgaatttgt ccctgttggc ctgctctctg acattgcgca tagttttcac atgcaaaccg
    11281 ctcaggtcgg catcatgttg acaatttatg catgggtagt ggcgctaatg tcattgcctt
    11341 ttatgttaat gaccagccag gttgaacgcc gcaaattact gatctgcctg tttgtggtgt
    11401 ttattgccag ccacgtactg tcgtttttat cgtggagttt taccgttctg gtgatcagtc
    11461 gcattggtgt ggcttttgca catgcgattt tctggtcgat tacggcgtct ctggcgatcc
    11521 gtatggctcc ggccgggaag cgagcacagg cattgagttt aattgccacc ggtacagcac
    11581 tggcaatggt attaggttta cctctcgggc gcattgtggg gcagtatttt ggctggcgaa
    11641 tgaccttctt cgcgattggt atcggggcgc ttcttaccct tttgtgcctg attaagttac
    11701 ttcccttgct gcccagtgag cattccggtt cattgaaaag cctcccgcta ttgttccgcc
    11761 gcccggcatt gatgagcatt tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg
    11821 catacagcta tatcgagcct tttgtacaaa acattgcggg attcagcgcc aactttgcca
    11881 cggcattact gttattactc ggtggtgcgg gcattattgg cagcgtgatt ttcggtaaac
    11941 tgggtaatca gtatgcgtct gcgttggtga gtacagcgat tgcgctattg ctggtgtgcc
    12001 tggcactgct gctacctgcg gcgaacagtg aaatacacct cggggtgctg agtattttct
    12061 gggggatcgc gatgatgatc atcgggcttg gtatgcaggt taaagtgctg gcgctggcac
    12121 cagatgccac cgacgtcgcg atggcgctat tctccgggat atttaatatt ggaatcggcg
    12181 cgggtgcgtt ggtaggtaat caggtgagtc tgcactggtc aatgtcgatg attggttatg
    12241 tgggcgcggt gcctgctttt gctgcgttaa tatggtcaat catcattttt cgccgctggc
    12301 cagtgacact cgaagaacag acgcaatagt tgaaaggccc attcgggcct tttttaatga
    12361 tacgttttaa tgatttccag gatgccgtta ataataaact gcacgcccat acataccagc
    12421 aggaatccca tcagacggga gatcgcttca ataccaccct tgcccaccag ccgcataatt
    12481 gcgccagagc tgcgtagact tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc
    12541 ggcgcaacca tcagtaccca atcagcgaag gttgaactct gacgcactgt ggaggccgag
    12601 ctaataatca tcgcaatggt tcccggaccg gcggtacttg gcattgccag tggtacaaaa
    12661 gcaatattgg cactgggttc atcttccagc tcttccgact tgcttttcac ttccggtgaa
    12721 tcaatcgctt tctgttgcgg aaagagcatc cgaaaaccga taaacgcgac gatcaaaccg
    12781 cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat ccatcaccag ttgcccggcg
    12841 taatacgcca ccatcatgat ggcaaatacg tacaccgagg ccatcaacga ctgacgatta
    12901 cgttcggcac tgttcatgtt gcctgccagg ccaagaaata acgcgacagt tgttaatggg
    12961 ttagctaacg gcagtaacac caccagcccc aggcctattg ctttaaacaa atctaacatt
    13021 ggtggttgtt atcctgtgta tctggtttat cagcgaaaag tataaggggt aaacaaggat
    13081 aaagtgtcac tctttagcta accttgcatc gcattgacca aaacttgaac cgatttagca
    13141 aaacgtggca tcggtcaatt cattcatttg acttatactt gcctgggcaa tattatcccc
    13201 tgcaactaat tacttgccag ggcaactaat gtgaaaagta ccagcgatct gttcaatgaa
    13261 attattccat tgggtcgttt aatccatatg gttaatcaga agaaagatcg cctgcttaac
    13321 gagtatctgt ctccgctgga tattaccgcg gcacagttta aggtgctctg ctctatccgc
    13381 tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt tgtcggtcga cctgggggca
    13441 ctgacccgta tgctggatcg cctggtctgt aaaggctggg tggaaaggtt gccgaacccg
    13501 aatgataagc gcggcgtact ggtaaaactt accaccagcg gcgcggcaat atgtgaacaa
    13561 tgccatcaat tagttggcca ggacctgcat caagaattaa caaaaaacct gacggcggac
    13621 gaagtggcaa cacttgagca tttgcttaag aaagtcctgc cgtaaacgaa aaaagaggta
    13681 tgacgatgtc cagacgcaat actgacgcta ttaccattca tagcattttg gactggatcg
    13741 aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc agagcgttcg ggttactcca
    13801 aatggcacct gcaacggatg tttaaaaaag aaaccggtca ttcattaggc caatacatcc
    13861 gtagccgtaa gatgacggaa atcgcgcaaa agctgaagga aagtaacgag ccgatactct
    13921 atctggcaga acgatatggc ttcgagtccc aacaaactct gacccgaacc ttcaaaaatt
    13981 actttgatgt tccgccgcat aaataccgga tgaccaatat gcagggtgaa tcgcgctttt
    14041 tacatccatt aaatcattac aacaactagt tgataacgtg acaacgtcac tgaggcaatc
    14101 atgaaaccac ttttatccgc aatagcaact gcgcttattc tcttttctgc gcagggcgtt
    14161 gcggaacaaa ccacgcagcc ggttgttact tcctgtggca atgtcgtggt tgttccccca
    14221 tcgcaggaac aaccaccgtt tgatttaaat cacatgggta ctggcagtga taagtcggat
    14281 gcgctcggcg tgccctatta taatcaacac gctatgtagt ttgttctggc cccgacatct
    14341 cggggcttat taacttccca cttttaccgc tttacgccac cgcaagccaa atacattgat
    14401 atacagcccg gtcataatga gcaccgcacc gaaaaattgc agacccgtta agcgttcatc
    14461 cagcaatagt gccgcacttg ccagtccaac tacaggcacc agtaacgata acggtgcaac
    14521 ccgccaggtt tcatagcgtc caagcagtgt tccccagatc ccataaccaa caattgtcgc
    14581 tacaaatgcc agatacatca gagacaagat ggtggtcata tcgatagtaa ccagactgtg
    14641 aatcatggtt gcggaaccat cgagaatcag cgaggcagca aagaagggaa tgattgggat
    14701 taatgcgctc cagattacca gcgacatcac cgccggacgc gttgcgtgcg acatgatctt
    14761 tttattgaag atgttgccac acgcccaact aaatgctgcc gccagggtca acataaagcc
    14821 gagcatcgcc acatgctgac cgttcagact atcttcgatt aacaccagta cgccaaaaat
    14881 cgctaaggcg atccccgtca attgtttgcc atgcagtcgc tcaccgaaag taaacgcgcc
    14941 aagcacgata gtaaaaaacg cctgtgcctg taacaccagc gaagccagtc cagcaggcat
    15001 accaaagtta atggcgcaga acagaaaagc aaactgcgca aaactgatgg ttaatccata
    15061 ccccagcagc aaattcagtg gtactttcgg tcgtgcgaca aaaaagatgg ccgggaaagc
    15121 aaccagcata aagcgcaaac cggccagcat cagcggtggc atgttatgaa gccccacttt
    15181 gatgaccaca aaatttagcc cccatacaac cactaccagt agcgccaaca ccccatcttt
    15241 tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag caatcaactt agctgaattt
    15301 acttttcttt aacagttgat tcgttagtca ccgtttacga cggcattaat gcgcaaataa
    15361 gtcgctatac ttcggatttt tgccatgcta ttcctttaca tctctaaaac aaaacataac
    15421 gaaacgcact gccggacaga caaatgaact tatccctacg acgctctacc agcgcccttc
    15481 ttgcctcgtc gttgttatta accatcggac gcggcgctac gctgccgttt atgaccattt
    15541 acttgagtcg ccagtacagc ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc
    15601 tcactattgg cgtcgttttt agcctcggtt ttggtatcct ggcggataag ttcgacaaga
    15661 aacgctatat gttactggca attaccgcct tcgctagcgg ttttattgcc attcctttag
    15721 tgaataacgt gacgctggtg gtgctctttt ttgcccttat taactgcgcc tactccgttt
    15781 ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaga
    15841 ttttctcaat caactacacc atgctcaaca ttggctggac catcggtccg ccactcggca
    15901 cgctgttggt gatgcagagc atcaatctgc ccttctggct ggcagctatc tgttccgcgt
    15961 ttcccatgct tttcattcaa atttgggtaa agcgcagcga gaaaatcatc gccacggaaa
    16021 caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
    16081 gctctggctt tctggcttct tttgttagcg gcgcatttgc ttcatgcatt tcacaatatg
    16141 tgatggtgat tgctgatggt gattttgccg aaaaggtggt cgcggttgtt cttccggtga
    16201 atgctgccat ggtagttacg ttgcaatatt ccgtaggccg ccgccttaac ccggctaaca
    16261 tccgcgcgct gatgacagca ggcaccttct gtttcgtcat cgggctggtc ggttttattt
    16321 tttccggcaa cagcctgcta atgtggggta tgtcagctgc ggtatttact gtcggtgaaa
    16381 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
    16441 ccagctattt ttccgcccag tctttaggct ggcttggtgc tgcgattaac ccattagtga
    16501 gtggcatagt acttaccagt ctgccgcctt tctcgctgtt tatcatctta gcgttggtga
    16561 tcgttgttgc gtgggtgctg atgttaaaag gaattcgtgc aagaccgtgg gggcagcccg
    16621 cgctttgttg atttaagtcg aacacaataa gaatttaatt cagccttcgt ttaggttacc
    16681 tctgctaata tctttctcat tgagatgaaa attaaggtaa gcgaggaaac acaccacacc
    16741 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
    16801 ttttattttc tgggtttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
    16861 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
    16921 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
    16981 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
    17041 atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca ttttgttcgt
    17101 caataaacat gcagcgattc cttccggttt gcttaccctc atacattgcc ctgtccgctc
    17161 ttccaatgac cacatccaga ggctcttcag gaaatgcacg actcacacca gcagtcacgg
    17221 taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac tggcaaattc
    17281 tgacacctgc acgacatgct tcttcatcat tagtcgcttt gacaataatg ataaattctt
    17341 cgcccccgta gcgataaacc gtttcgtaat cgcgcgtcca actggctaag taagttgcga
    17401 gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc aatttaaatc
    17461 ggtctatatc caacaacatt aaataaagat tcagaggttc agcattgcgt aactgatgat
    17521 cgaaggattc atcaagaacc cgacgacccg gcaatcccgt cagaacatcc atattgctac
    17581 ggaccgtaag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa agcaaccctt
    17641 cctgaaaagc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc agcattaatt
    17701 cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg taaggtaatt
    17761 catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac agtccataag
    17821 aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg tgaaacatac
    17881 ttaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag atggcatcaa
    17941 tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg cgcgctattc
    18001 taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt tatgagcatc
    18061 tctgagataa cattgtgatt taaaacaaaa tcagcagata aaaaagtgtt taattctata
    18121 aattaactct gcattatcgc aattaaaagg atgacaaata gcataaccca ataccctaat
    18181 ggcccagtag ttcaggccat caggctaatt tatttttatt tctgcaaatg agtgacccga
    18241 acgacggccg gcgcgctttt cttatccaga ctgccgctaa tgttgatcat ctggtccggc
    18301 tgaatttgaa aattattata acggaagagt atacaaaaaa agtaattttg ctggcgactg
    18361 cgaaacggca ttcgcattag cggtgaagtt tgccctcgcc cagcggctgc aattactgct
    18421 ataacgaggc tggtctggcg ataaatccag gccagccatt ggtggtgttt atacgttcaa
    18481 gccacgatgt tgcaacatcg gcataatctg aggtgcctta ccgcgccatt gtcggtacag
    18541 gcgttccaga tcttcgctgt tacctctgga aagaatcgcc tcgcgaaaac gccgcccatt
    18601 ttcacgcgtt aatccgccct gctcaacaaa ccactggtaa ccatcatcgg ccaacatttg
    18661 cgtccacaga taagcgtaat aacctgcggc atatccgcca ccaaaaatat gggcgaaata
    18721 actgctgcga tagcgtggcg gtatagcagg aagatccata ttttccgcca ccagcgcccg
    18781 caattcgaaa tcatcgacat cctgcattgc ttcgttttct tccaggcaat gccagcgcat
    18841 atcgagaagt gcggcgctaa gcagttcgct catctcatac cctttattga acaggctggc
    18901 attacgcatt ttctgttgaa gttcgtcagg cattgcggtc ccgctctgat aatgccgggc
    18961 gtagcgagcg aatacctgcg gatgtgttgc ccagtgttcg ttgatttgcg acggaaattc
    19021 gacaaaatca cgcggcgtgt tggtgccgga aagcgtggca taacgctggc gggcaaaaag
    19081 gccgtgcagc gtatgaccaa attcatggaa taaggttatt acatcatccc agagtaacaa
    19141 cgcaggctca ccggcagcgg gtttctgata attgcagacg ttataaatta ccgggtgcgt
    19201 ttcattcagc gttgattgct caacaaaatt gcccatccat gcgccgccgc tttttgaatc
    19261 acgggcgaag aaatcaccgt aaaataacgc cagccccacg ccattatgat caaaaatttc
    19321 ccacacacga acgtcaggat ggtagacagg aatatcaaaa cgttcgacaa acttaatacc
    19381 gaagagctga ttcgcagtcc agaatacgcc ttcattcaac accgtgttta attcaaaata
    19441 tggcttgagc tgcgcttcat cgagatcgaa tttctcacgt cttacctgct cggcataaaa
    19501 tgcccagtcc cacggctgcg cgctaaaccc gccctgctgc ttatcgataa ccgcctgtat
    19561 ggaggctaat tcatcgctcg cacgttgacg cgccgctgga acaatttccc gcataaagtt
    19621 gagcgctgct tctggcgttt ttgccatctg atcggcgatt ttccatgcgg cataatgagg
    19681 aaaaccaagc agtttcgcct gctgcgcgcg gatctccacc agacgttgaa tgatagcgcg
    19741 ggtatcattg gcatcatttt tttccgctcg cctccagccc gcagtaaaca gtttttcacg
    19801 cgtcgcgcga tcgcgcagtt cagcaagcgc tggttgctgg gtggtattca gcagcggaat
    19861 cagccagttg ttatccagac ctttctcgcg agccgcctct gccgccagcg caatctcttg
    19921 ttcactcatt cctgccagct gcgcgatatc gttcacaacc agaccgccgg atttatttgc
    19981 tgccagtaac cgctggttaa actggctggt cagggtcgca gcttctgtat tcagtacttt
    20041 taattttgct ttatcagctt gctcaagttt ggctccggca aggacaaaac gttgatgaat
    20101 cacctccacc aggcggatgg attcactatc aagccccagg gattcacggc gttgccagac
    20161 agcatctacc cgcgcgaata attccccgtt cagatagata tcattcgcaa gttccgccag
    20221 ttcagcggaa aactgttcat caagacgctg taattcatca ttggtatgcg ccgcagtcat
    20281 cgcaaaaaag acgctggtaa cgcgggtaag taattctccg ctttgttcca gtgccagaat
    20341 agtattgttg aaatcaggtg tttgcgggtt aagcgcgatg gcagcaattt ctgcccgctt
    20401 ttgctgcatt ccctcatcga atgccgggcg atagtgatga ttggcaattt gatcaaaatg
    20461 gggagccaga tacggcagtg tgctttgcac aaggaaagga ttcattgttg tcattttctt
    20521 ctcctgaacg cgaggtgttc catagcgtag gcttactgat aacggagtgc aatcttgcaa
    20581 accagtatta cccgctctta agcatcacgt gctatgttat tgacacacaa aatcgttgag
    20641 gaacagtgag atgatcgttt tagtaactgg agcaacggca ggttttggtg aatgcattac
    20701 tcgtcgtttt attcaacaag ggcataaagt tatcgccact ggtcgtcgcc aggagcggtt
    20761 gcaggagtta aaagacgaac tgggagataa tctgtatatc gcccaactgg acgttcgcaa
    20821 ccgcgccgct attgaagaga tgctggcatc gctccctgcc gagtggagca atattgatat
    20881 cctggtaaac aatgccggcc tggcgttggg catggagcct gctcataaag ccagcattga
    20941 agactgggaa acgatgattg ataccaacaa caaaggcctg gtatatatga cgcgcgccgt
    21001 cttaccgggt atggttgaac gtaatcatgg tcatattatt aacattggct caacggcagg
    21061 tagctggccg tatgccggtg gtaacgttta cggtgcgacg aaagcgtttg ttcgtcaatt
    21121 tagcctgaat ctgcgtacgg acctgcatgg tacggcggtg cgcgtcaccg acatcgaacc
    21181 gggtctggtg ggtggcaccg agttttccaa tgtccgcttt aaaggcgatg acggtaaagc
    21241 ggaaaaaacc tatcaaaata ccgttgcatt gacgccagaa gatgtcagcg aagccgtctg
    21301 gtgggtgtca acgctgcctg ctcacgtcaa tatcaataca ctggaaatga tgccggttac
    21361 ccaaagctat gccggactga atgtccaccg tcagtaattt ttatacccgg cgtaactgcc
    21421 gggttattgc ttgtcacaaa aaagtggtag actcatgcag ttaactcact cacaagcaag
    21481 aacgaatgac cgtcgaaacg caacttaatc ccacacagcc tgtcaatcag cagatttatc
    21541 gtattcttcg ccgcgacatt gtccattgcc tgattgcacc aggcacaccg ttgtcggaaa
    21601 aagaagtttc tgttcgtttc aatgtgtcac gccagccggt tcgtgaagcc tttattaaac
    21661 tggcggaaaa cggtctgatt caaattcgtc cgcagcgtgg cagctacgtc aacaaaattt
    21721 ccatggccca ggtgcgcaac ggcagtttta tccgtcaggc cattgagtgc gcggtggcgc
    21781 gtcgggcggc gagcatgatt accgaaagcc agtgctacca actggaacaa aatcttcacc
    21841 agcaacgcat tgccattgag cgcaagcaac tggatgattt ttttgaactt gatgacaact
    21901 tccatcaact cctgacgcag attgccgact gtcaactggc gtgggatacc attgagaacc
    21961 tgaaagcgac cgttgatcgc gtgcgctata tgagcttcga ccacgtttct ccaccagaaa
    22021 tgctgttacg ccagcatctt gatattttct ctgccctgca aaaacgtgat ggcgatgcgg
    22081 tagaacgtgc aatgacgcaa catttgcagg aaatcagcga atccgtgcgc cagatccgcc
    22141 aggaaaacag cgactggttt agcgaagagt aattcatttc ctctcatccc atccggggtg
    22201 agagtctttc cccccgactt atggctcatg catgcatcaa aaaagatgtg agcttgatca
    22261 aaaacaaaaa atatttcact cgacaggagt atttatattg cgcccgttac gtgggcttcg
    22321 actgtaaatc agaaaggaga aaacacctat gacgacctac gatcgtaacc gtaacgcaat
    22381 caccactggc agccgtgtta tggttagcgg caccggtcac actggcaaga tcctgtcgat
    22441 tgatactgaa ggtctgaccg ctgagcaaat ccgccgcgga aaaaccgtag ttgttgaagg
    22501 ttgtgaagag aaactggcac cactggacct gattcgtctc ggcatgaact aagcgtgtga
    22561 atgccgccga tggcggcatt gcttttttta cttcacggaa tatttcgcca ctgtcgcttt
    22621 cgcgccatgc gctaataaag acaagtacgc ttccgtcact ttagtcgtaa acaagctatt
    22681 atctggcaaa tcatcaccaa atatcgcctt aatcgccaac aatgactgca cgcgcgctgt
    22741 cccttcagca ctactttgta ctgccttctg aataacaggt aacagtgggt cactgatttc
    22801 tatcggattt ccctgttcat caacaccacc gacataacgc atccaacccg cgacgcccag
    22861 cgccagcaga tcgaacttgc tgtcatgcgc cagatgccag cgaacagaat ccaacatccg
    22921 ctgtggcaat ttctggctgc catccatcgc aatctgccag gttcgatgac gtaacgccgg
    22981 gttgctatag cgttcaatta atcggttagc gtaatcttgc aaatcaacgc cctgcacttt
    23041 cagcgtcggc gcttgttcct gcaacattaa ggtatacgcc gcatgacgat aatgttcatc
    23101 ctccatgcag tcattaatgt gctgatatcc ggcaagatag cccagatacg ccaggaatga
    23161 atgactgcca ttgagcatgc gcaacttcat ctcttcataa ggcagcacat cactaaccag
    23221 ttcgactccc gctttttccc attccggacg tccggcaaca aagttatctt ctattaccca
    23281 ctggcggaaa ggttcacagg caacgcctgc cggatcgcgc acaccggtaa gttgttcgat
    23341 tttcgccagc gtatcctctg tcactgcggg cacaatacgg tccaccattg ttgatgggaa
    23401 agtcacgtta tcttcgatcc attgtgccag ttttacatca atggcttgcg cataggaagt
    23461 gacaacgtca cgcataacat gaccgttttc tggcatgttg tcacatgaca tgacggtaaa
    23521 tgcgggaagt cctgccgctt tacggcgagc cagcgcctca acaatcaccc ctgttgcagt
    23581 tttcggctgg tggggatttt gcacgtcggc aacgaccatc gggtgatcga gcattaactg
    23641 tccggtcgcc ggagagtgga aatacccttt ttcggtgatt gtcagagaga caatcgcgat
    23701 ttgcggttca cacatcgcag ccaacacggt ttctaagccg tctatttgta cgtgtaaggc
    23761 ttttttaacg acgccaacga cgcgagccgt ccacgcatca gccgacattt ccgcaacggt
    23821 ataaagatta tcttgctgtt gtaaatcggc aatttgctgt tcgccgccga ttaagttgac
    23881 ctcataatat ccccagtcac tgaaatgttc cgtagcaaga atatcggcat acacgccctg
    23941 atgcgcacgg tgaaatgcac caaagcctaa atgaacaatt cttggggcca ggttattacg
    24001 atcataaaca gggagcgtcg cttttgctga taacaaatga tttcccataa caattcctta
    24061 aaattaaata tagcaagcta catgtttttt tatatgaata aaaatcccct ctccggtaag
    24121 agaagggatt gaggatttac agacttctgg acggttgcgc agctcttaca acatgtggtt
    24181 gatcttctgc ggcgtcttgc agcgcactta aatcacggtc tttcacttct ggcattttca
    24241 gcgcagagat taaaccaatc acagaatatg ccatgatcat aatggcgatc ggataccagg
    24301 attccgtcat ggtgcagaaa atccccgcca ggataggacc aaaaccggaa gcgataagac
    24361 caccaatttc tttagaaata gccattcggg taaagcggtt tttacagccg aacatttctg
    24421 ccatggtaat gttttccaga gcaaataatc caagcaccgc acagttatga atcacaatca
    24481 gtgcaaccat aatggtgctc ggggcatagc ttttatctac gatgatagaa agcattggcc
    24541 atgccagcac aatcgcagag gtattcataa taatatacgg gatccggcga ccaattttat
    24601 cggataacca accaaggaac ggaatggtca taaagccgag aatcgaactg atcatcaatg
    24661 catctgttgg aattgctttg ttaaacaata acgtctgcac taaatagcct gcaaggaaag
    24721 tctgaattaa acctgagtta ccagcctgac caaaacgcag ccctgttgcc agccagaagg
    24781 atttgctctg gaacatgcta ccagcaggtg caggttttgc tgtcggttgg ttgctgtcgt
    24841 taaccttctc aaagaccggg ctttctttca gattcatacg caaccagata gcaaagacca
    24901 tcacgacaac gctcgccagg aaaggtatac gccatcccca cgccagcagt tcctctttac
    24961 tgagaatgaa gaacataaag gcccagattg ccgttgcgct caaggttccg cagttagttc
    25021 ccatagccac aaatgaggag ataattccgc gcttaccttt tggcgcatat tccgccagca
    25081 tcgtaccggc accggaaatt tccgcacctg cacccaaccc ctgaataata cgcagcgtca
    25141 ccagcaagat gggggcaaaa acaccaattt gtgcataagt cggtaacaca ccaattaagg
    25201 tggtacagat ccccatcatg gtgatggtaa taaagagcac ttttttacgc cctattctgt
    25261 cgcccatttt gccgaaaata aatgctccga caatacgtgc cacataacct gcaccatagg
    25321 ttcccattgc cagaattaac gccattgccg ttgatgattc aggaaaaaat atttcatgaa
    25381 acactaacgc tgcgccgagc gaatataact ggaaatccat aaattcaagc gcagtgccca
    25441 gccatcccga tactgctgct ttcaccaaat ccttagtgct tctttcgtgt ttttctattg
    25501 tcataatggt tatctctaaa aaagaggtaa gatgcgtact acttattcgc cgttattggt
    25561 attattcaga aaaagtgagt aagactttgc agcaatgttt ttgatcctgt tcaaataaac
    25621 taatggcatc cgcaacatgc tggaaatcaa acgtatgggt aattagtttt tctggtttaa
    25681 ttaacccttt acttaaccag tcgataacga ccgggaattt atttgcattt aagcgtgaag
    25741 agaaaataga gagttctttt ccggtaattc cttgctgaat cacttcagac ggttcactgg
    25801 agaagcccat caatacaata cgtgccgctg gagaagccag cgttacggcc tctttcaaga
    25861 ttgaaggatg acaagcggca tcgataacta atgtcggttg gatgcctttt tcatcgaaaa
    25921 tctcgccaag tggtgtctgg ctgttattaa tcgcccagtc tgccccgctc tctttcgctt
    25981 tttccagtcg ttcatcaatg cgatcggcaa caatcacatt tttaacgtta tagacgcctt
    26041 ttaatacctg aacgatcgtc aggccgattg gaccggcacc gtaaaccaga acggtatcat
    26101 tttcagtcgg ttgaccatga ccggtaacgt tagccgcaat ggtaaaaggt tcgatcatca
    26161 ccgcatattg atcggccact gcttcaggaa ttttccacgc attttttgcc ggaaccacgg
    26221 catattcact gaaaccaccg tcagcgtgca cacctaatac agcaagtgtc gtacaaacgt
    26281 tcggcttacc tatagagcac ggatagcaat gcccacagct gaccaccgga tcgacagcaa
    26341 cgcgttcacc gactctggcg ctttccacgc cttcacccac tgcatcaatg acgccaaaga
    26401 attcatgacc aatgacgcgc ggatatttcg caaaaggatt atgcccacgg taaatatggc
    26461 tatctgaacc acaaattccg gcaagtttca cttttactcg tacttcaccc gctgacgggg
    26521 tgggtatttc acgttcgata atcgacagtt gattcggttt ttcaattaaa atgcttttca
    26581 ttaccttact ccttaccagt tccacagcgt gccatcttcc agacgtgcga ctggcagata
    26641 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatgc caagacccgg
    26701 tttttctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
    26761 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
    26821 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
    26881 tacctgatac agcgaagcaa aatcggcaat ccgacgcata ccggtaattc cgcctgcatg
    26941 agtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
    27001 gatgctgttg aagacttcac ccaccgcgat gggtgtgacg gtatgttggc gaatgagacg
    27061 gaagcattcc tggttttccg caggcgtcgg gtcttccatc cagaacatgc gataatcttc
    27121 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
    27181 caaatgttca ttaaatccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
    27241 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
    27301 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
    27361 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
    27421 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
    27481 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
    27541 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
    27601 aaactgccag atatcttcga tacggtgtgc atcgcgacca ataagctgcg gacaaaggtg
    27661 atcctgcaaa taagaggcca cggaaagctc acgtccattt agggtggcat ccccaaggcc
    27721 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
    27781 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
    27841 gaaatctacg caactgaact actaccatac aagtatgaag atcaaaaaaa gccggagtga
    27901 tcacaaaaaa aggcgtatat ttgcgccgtg aatggttgac aaaagatgaa atagaatacc
    27961 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
    28021 gcccccagcc cgcaacaatg atcaacatgc cgcaaagcgc aattaacgcc cccgtccagt
    28081 cataaagagt cagtttcacg ccatccacaa cgcgcagcca catcaacgcc gtgcaaacat
    28141 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
    28201 agacaaacag tgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
    28261 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
    28321 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
    28381 taatatcatc aatataaaca ccctccttga acgttttgcg ttcgcagtgg atggtgtaga
    28441 atcagcttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
    28501 ggcctgctcg cttttctgct gccttgcgca ctggtattga gcacaactgt tcatgccgaa
    28561 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagcg cgccgctatg
    28621 gaaaaagagc aatggaatga cacgcgtaat ctgcgccaga aagtgaataa acgcactgaa
    28681 aaagagtggg ataaagccga cgctgctttt gataaccgcg ataaatgtga gcaaagcgcc
    28741 aacatcaatg cctactggga gccaaatact ttgcgctgcc tggaccgtcg aactggccgc
    28801 gttattatcc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
    28861 ccacagtgtt aagctacgcc cgctggagcg tgaagattta cgctatgtac atcaactcga
    28921 caataacgcc agtgtgatgc gttactggtt tgaggaaccc tacgaagcct ttgttgaact
    28981 ctctgatctg tatgataagc atattcacga tcagagcgaa cggcgctttg tggtggaatg
    29041 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgcgc
    29101 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
    29161 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
    29221 tgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
    29281 tgaattgatg catgagttct ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
    29341 attccagcat cagtatctgg ctgagcacaa aacaccgggt cagactctcc tgaagccgac
    29401 cgcacaatag tattaataat aatcgatcgt atttttgatg gtataaaccc gttcgacggc
    29461 gggttttact ccttcatcaa caatgattag ctgacagtcc accggattgg cgtgattgtc
    29521 atattcacag ctctgtttta cattaccaac ccgttgatta ttcagtaaag taacagcagt
    29581 gtaatctaat tttttgatcg ggtccgttga tggcgtggcg ctgacagata atgttttatc
    29641 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
    29701 catctgtttg ctgctggctt taatcacgaa accattatca tcggtttccc aactcacccc
    29761 ggcagaaggt aattctgcta gctggcattt tccctgcaaa cgaactcttt tctccagcgt
    29821 ctcggcatca cggtaataat tggcatccag taccagggca accaaggtat tattttccag
    29881 atccagtaat tcgagagaat caaaacagcc ttcttccgac aaagtccccg aaacacgttt
    29941 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttacgg gaccacgcag
    30001 cggatcaaaa tcgaattcat tagagaaact ggccatctcc ggggtaaatg aaagtgttac
    30061 ctctgtgcgg tcacatcctg tgagtaatat cgcgagtaag catggcagta atttgtattt
    30121 cacaacagtc acctgagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
    30181 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaacact
    30241 caacctactg tgccgcgctt ctgctcgccc tcgcctcacc cacggtactc gccgcgccgg
    30301 gatcctgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
    30361 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
    30421 tcgtcgggca ttgcgctaat gatacgcata aaattctcta cacccgtacc accagcggta
    30481 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
    30541 ttatcgactg aacgccggat gcgacacatc cggcgatttg aacattcaac ataaccccac
    30601 cttattactc atacccttct attgatatgg attaataatt attaacccca aatgggtaga
    30661 ttccctctat t
//
LOCUS       DAPQZD010000047        30231 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPQZD010000047 DAPQZD010000000
VERSION     DAPQZD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.047"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(89..367)
                     /locus_tag="RO454_002027"
     CDS             complement(89..367)
                     /locus_tag="RO454_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933865.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(726..1016)
                     /locus_tag="RO454_002028"
     CDS             complement(726..1016)
                     /locus_tag="RO454_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0933866.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1017..1328)
                     /locus_tag="RO454_002029"
     CDS             complement(1017..1328)
                     /locus_tag="RO454_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDY0933867.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1557..2465
                     /locus_tag="RO454_002030"
     CDS             1557..2465
                     /locus_tag="RO454_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0933868.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2529..3470)
                     /gene="fabY"
                     /locus_tag="RO454_002031"
                     /gene_synonym="yiiD"
     CDS             complement(2529..3470)
                     /gene="fabY"
                     /locus_tag="RO454_002031"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDY0933869.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3515..3952)
                     /gene="dtd"
                     /locus_tag="RO454_002032"
     CDS             complement(3515..3952)
                     /gene="dtd"
                     /locus_tag="RO454_002032"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDY0933870.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3949..4821)
                     /locus_tag="RO454_002033"
     CDS             complement(3949..4821)
                     /locus_tag="RO454_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDY0933871.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4815..5414)
                     /gene="yihX"
                     /locus_tag="RO454_002034"
     CDS             complement(4815..5414)
                     /gene="yihX"
                     /locus_tag="RO454_002034"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDY0933872.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLRAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5555..6442)
                     /locus_tag="RO454_002035"
     CDS             complement(5555..6442)
                     /locus_tag="RO454_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDY0933873.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLTSPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6432..7781)
                     /locus_tag="RO454_002036"
     CDS             complement(6432..7781)
                     /locus_tag="RO454_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDY0933874.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7880..9313)
                     /locus_tag="RO454_002037"
     CDS             complement(7880..9313)
                     /locus_tag="RO454_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933875.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9383..10393)
                     /locus_tag="RO454_002038"
     CDS             complement(9383..10393)
                     /locus_tag="RO454_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDY0933876.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10426..11313)
                     /locus_tag="RO454_002039"
     CDS             complement(10426..11313)
                     /locus_tag="RO454_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDY0933877.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11338..12216)
                     /gene="yihU"
                     /locus_tag="RO454_002040"
     CDS             complement(11338..12216)
                     /gene="yihU"
                     /locus_tag="RO454_002040"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDY0933878.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12389..13285
                     /locus_tag="RO454_002041"
     CDS             12389..13285
                     /locus_tag="RO454_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0933879.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13325..14113
                     /locus_tag="RO454_002042"
     CDS             13325..14113
                     /locus_tag="RO454_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0933880.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14221..16044)
                     /gene="typA"
                     /locus_tag="RO454_002043"
     CDS             complement(14221..16044)
                     /gene="typA"
                     /locus_tag="RO454_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDY0933881.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            16417..17826
                     /gene="glnA"
                     /locus_tag="RO454_002044"
     CDS             16417..17826
                     /gene="glnA"
                     /locus_tag="RO454_002044"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDY0933882.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18000..19049
                     /gene="glnL"
                     /locus_tag="RO454_002045"
     CDS             18000..19049
                     /gene="glnL"
                     /locus_tag="RO454_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDY0933883.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19061..20470
                     /gene="glnG"
                     /locus_tag="RO454_002046"
     CDS             19061..20470
                     /gene="glnG"
                     /locus_tag="RO454_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDY0933884.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            20582..20692
                     /locus_tag="RO454_002047"
     CDS             20582..20692
                     /locus_tag="RO454_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDY0933885.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(20925..22298)
                     /gene="hemN"
                     /locus_tag="RO454_002048"
     CDS             complement(20925..22298)
                     /gene="hemN"
                     /locus_tag="RO454_002048"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDY0933886.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(22487..22996)
                     /gene="yihI"
                     /locus_tag="RO454_002049"
     CDS             complement(22487..22996)
                     /gene="yihI"
                     /locus_tag="RO454_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDY0933887.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            23578..24210
                     /locus_tag="RO454_002050"
     CDS             23578..24210
                     /locus_tag="RO454_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDY0933888.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(24592..27378)
                     /gene="polA"
                     /locus_tag="RO454_002051"
     CDS             complement(24592..27378)
                     /gene="polA"
                     /locus_tag="RO454_002051"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDY0933889.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            27736..28668
                     /locus_tag="RO454_002052"
     CDS             27736..28668
                     /locus_tag="RO454_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDY0933890.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVRGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(28709..30139)
                     /locus_tag="RO454_002053"
                     /pseudo
     CDS             complement(28709..30139)
                     /locus_tag="RO454_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
BASE COUNT         7515 a         7797 c         7341 g         7578 t
ORIGIN      
        1 aagtcagcag ctttaccgca ccaggggtta aggtgaaatc taccattcat aacgaccggt
       61 cagtgccgca ccaaccggtc tttaattgtt agtagtagta aatttgtgga taacagtccg
      121 ggaaccagac gattccttcc ggatcatccc cctgcattac ccgatgacat tggcgtcgat
      181 catctgcgcg acggcgaagc gtagtataga gccgcttcca ttcatgatcg cccggtgtac
      241 tgtagtttac ccaacaatcc attgaagggt gacgccattt acctgtaagt gttgggatcc
      301 ctgaatgctt atgcggcttc gctttaccat tacgatccct atctttacgc gtattaaacg
      361 tggacatttt tagtctcctg ttaagagttc taaaaatgtg cttccttaaa atagttcatc
      421 attttccctt aattcgcata gcgtgctttt gcacgaattt ccgatacgcg gggaccatta
      481 tgatagcgtc gatgcccaca catccaacaa gaacaaatac agggagtacg aaagcaaata
      541 cctgatgctt ttttgcttcc atcgcctgca gtgcgataac gacagcggat tttttttaat
      601 cgcaccatat gatggcgacg ttcagcacga ttacgggtca tagcaaaact cctgaacagg
      661 cggcactatg ccgctttcat aggagttctg aatgctggtg ctttctgtat ggcataacca
      721 tgcctctaac ggttaagtgc ttgcagcaca tgttgcggat cattggcaat cgctcgcagc
      781 aaagcctttg caggcccggt cggtgaacgt cgcccctgct cccagttacg caacgtgtct
      841 acgcttacgg aaataagatc ggcaaattta gattgtgaca accctgaagc gcggcgtatt
      901 tctttgattt tcatagcatc aacttcaaaa gtccgtgaag gctgtcgcgt tccttcaata
      961 atttcattca tctgggccat gctgtgagtc aaccgctcaa aaagtgcctt atccatttac
     1021 cacctcgtat ttaacaaccg gagcattgct ttttcctggg gagacagatc atctttaata
     1081 cccttacgat aaataagcag taatctgatc tcataatgtt taacctgatg aaagtaaata
     1141 accctgacac cagcacgttt cccctttcct cctgagaccc atcgaacctt tcttaaccca
     1201 ccagtttcag gtattacctc accatagtca ggattcaagg ccaggaaaaa ctgaaaacga
     1261 ctgaattcat cgtcgttcag cagtttttga acatcttcag taaaaatttc cgtttcgatg
     1321 aagagcataa ttatacgcca ttggcttaga gcaacaatcc tttttgtacg tcattgacgt
     1381 accattgaaa ccactagctt actgatagtg ctccagcaaa accacgtctc tgccgggttt
     1441 gtctttacat ttgcgaggcg ctttccagat ttttcacgcc cgtgaatttc cctgtatcca
     1501 tccagcaaaa acgggtgtat aaaactgtac attcatacag caaaatgagt tctactatgg
     1561 cactggaaaa aggaattgca gagttagtcg aagagtttat cgctgcgggt cgcccctcat
     1621 cgcgccaaca aagtattacg cagcgcagag agggctatat cgccagcgct gtccttgcgg
     1681 gtgagacaga aacgcgggta gatattcaga ctatcgaact tgaagggatg accttacgaa
     1741 tagtgtcacc gctgaatgcc ccgacattac tcccgacgat aatttattac tacggtggct
     1801 gttttgttag cggcgggttc gcaactcacg acaaccagct gcgccagtta gcctattacg
     1861 ggcaatgtcg ggttattgcc gtccagtatc ggctggcacc ggaacatact ttccccgccg
     1921 cccatgatga tgcacaacga ggtgcagaat tagtccggca acacgccgaa cgactcggcg
     1981 ttgataaaca acgaataact cttgccggtg acagtgcggg agggcatctg gcgctggtaa
     2041 ctgctttgcg attgaagcgt gctggcgagt ggcaaccagc acaactcatc ttaatttatc
     2101 ctatgcttga tgcgactgcg catttcgaaa gctacatacg caatggtcac gattacatca
     2161 tcacacgcga cactttactg agcggctttg agatgtacct gccaggcatt gaacgcaggc
     2221 atcctgaagc cagccctatt tggcgcaatg atttcaatgg cttgccacct gtacatatca
     2281 ttaccgctga atacgaccca ctttgcgatg aaggtgaagc gttgtatcac cgcatgacag
     2341 ggcaaggagt gcaatgcacc tgccaacgct accttggcgt tatccacgga ttttttcagt
     2401 taggtggtat cagtgaggct gcgcgatcgg cgttacggga tgttgcctgg agagcgggaa
     2461 ggtaactccg gaccagggcc cagaaaaggt tttcagattg aaaacctttt cagtcaagta
     2521 aaacgcccct actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc
     2581 agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttctact
     2641 tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc acttaaggca
     2701 ccaaggtcgg ctaccgcatg gggttttccg ctaatcggct tgctgtagcg gatatgcgca
     2761 tccgccagaa taatcgttcc gccgaggtgg cgttcgcgca gcatcagcca gataagcccc
     2821 caaccggtga gcgtagccag cgagaataaa ctcccggcaa acagcgtgtg atgcggattc
     2881 tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg ctgaatgcgc
     2941 acacccattt tttcgcttaa cgggatatgt tcataccacg cttgctggag ctgggcgcac
     3001 cagtcgccgc gatgaagaat atcatccaga gtggcgacgg gtttaatcat cagaaaatgg
     3061 cgaatcggcg tggtggttgg cgtggtgatt tcaccctgat taacaaaccc cagcttggcg
     3121 aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc ttcctgacgt
     3181 gccaccgact ccagggtcat cgccatcagc gtgcctaacc ctttgtcctg cacgtcggga
     3241 tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacag tcggcctacc
     3301 gctaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg
     3361 tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca gcgaaactga
     3421 tagtaacgct ctaattcttc ttctgtttgt ggaacccgaa ggtgatacat agcgatactc
     3481 tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg
     3541 ggccatcatt gaccagcgat acctgcatat ccgcagcgaa gcgtcctgtt tgcgtgttca
     3601 tatcttgctg acggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg
     3661 atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggca agggtaaact
     3721 gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc ttgccttcgg
     3781 catcgctaaa gatgcggtag ccgagtacac gctcgcacag acggtttgct ttctgttcgt
     3841 cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttca cccgtcactt
     3901 ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc
     3961 gtcttcttct tgttcagcag cttgttttaa tttgcggtat tccccgagag tgacagtaat
     4021 ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat
     4081 caccgccagc acgccgtaaa tgagctgata tgacgggaac atggtgatat aaagcgcgaa
     4141 acctttcttt cctgcttcga acaggagcgc ggcgacaaac gcgccgacaa tcgcgtcgcg
     4201 gttaggtacg cgaatggtag gaacgatgct gtacagcaac caaaaggaga tccacgataa
     4261 cagcagcgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg
     4321 cagggaaagc agataggagc tgatcgccag actggcccct gccagcagcg gccccagcgt
     4381 taaaatcatc cagtacacgg caaacgagta aattttgggt cgcgctcgtt tactgcgcca
     4441 gatggtattc aacgcgctat cgatggagta catcaacaat aacgccgtga cgatcagccc
     4501 gcacgccccg acggcggtca ttttgttgga attggcgaca aactgttcga tatatcgctg
     4561 gataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgca actgaatgct
     4621 gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac
     4681 taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc
     4741 aatgcgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacgat gcctggcttt
     4801 gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct
     4861 ttcaccagaa tactagtaat gcctagccga ttagctcctt ctatattatc ggcgttatcg
     4921 tcgaaaaaga ccgtatcgtc tggtgaaaaa ccttctgccc gcaaaacatg ctgataaatt
     4981 cgtgcttcag gtttgcgcat acccaagtct tgcgacagat agatatggtc agcagcatca
     5041 cggatttccg ggtattcctc cggccagaag gtagtatgca ggcggttagt attggaaagc
     5101 accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccggtcgt
     5161 agcgcaacaa ataccgcctg ccaaccgtgg gagaactgct catagcttag cggcagggcc
     5221 atctcatgac acagtgcctc tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga
     5281 tgaaacgcct cgcccatgtg gaaactcttc ttaagcgtag ccagtggaac acgcgttaaa
     5341 tcgctccagg cccccagcac acggttaaag tcgatatcga caatcacatt acctaaatca
     5401 aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg
     5461 atgggtttgg ctatgtggca ataagggtaa tcttgtgatg ctgattgcga aaaagcagag
     5521 taatttcact ggctttttcg caatagatga atggttaaga ttcaggtgta aattgccaac
     5581 gagtttcgat gaccagcggc ttatctggtg tcacaaattc cggcgttaga tttattgcgt
     5641 taggtgcctg agtcagcggg ttaatacagg tggcatcggg ctgtttatca aataccacca
     5701 gactgtttgc agaagaggtc atggtcatcg cgagtttacc aggccacagt aacttaacct
     5761 tcaccccgtc ataaaaacca aaacagtcat cccagggacc aacttgagga gagatacgat
     5821 ttccagttgg cagttcatca ctacccgctt cttcttgcca gtccgcatca aaaagcacct
     5881 gcaacgattc tgtattttgc ggagtcagct ttttcgcaaa ccacggatgc cagcctgcgg
     5941 aggccgggaa tgtatcggca tatgaatgga cttccagttg caaaaccagc gcatcgttct
     6001 ccagcaaaaa agtctgaatc acttcccctt gccagggcca cggtgaagtc aggggcatac
     6061 gtaaagtcag ggaatctgct gttttgtcga tgatttccca cgttgagtaa caagccatac
     6121 catgcaacgc atgtggtggt ttattggcgg gtaaagaata acattgccca ccagcattca
     6181 gcgtcgcatt ccccagtcgc cctgcccacg gcaccattgg gaaacagcca tactgaaaag
     6241 ctctgcgctg cggctgccat tggcgtaata cttcagaacc aaatgccttt aacgaggtta
     6301 tcctggcacc atcatgcgga taaatctgca ggtgaatatt gcctttttcc agcgtcacta
     6361 actctcctac aggcccaggc catggcgcgt caaacgcctg ttgtagcgcc aggtaatgct
     6421 cgttttttcg ttcagttttc atgagattgt tccctgatgt agcggtaaga agcggtttct
     6481 tgttcatcgg ttggctcaat cagccagacg caaacaaagg aaagaactga gacagcaaat
     6541 aaaaacagag cgacatacca cggttttcct ccccctagcg ccagcaaact tgaggcgata
     6601 agggggctta gtccgccaag aatcgcggaa aattgataag caaaagaaag gccggtatat
     6661 cgtactttgg taccgaacat gcgggtgaaa agtgttggct gcacggcaaa catcatcgtc
     6721 ggacctaagt tgtagccaag gatcatactg caccagatga tgagcgtact tttgctatcc
     6781 agtaataaga agaaaggaaa cgcgaacagg acacaaaacc ctgctcccga caaatacaga
     6841 cttttttgac ctatacgatc tgataaccat ccaaccaatg gacaactgaa aataccgatc
     6901 gccgaggcaa tcaacatacc cgtcagggga atatctcgcg acaatgctaa ttggctggaa
     6961 atatagacaa taccaaaggc gttaataata ttagaggaga cactttccgc cagcctcgca
     7021 cccagtgcca ataaaatatt tcgtggatgt tttttgaata attcaatcaa cggaggaacg
     7081 gacttttctt tattattcgt cgttttttgc ttttgaaaat ccagcgtttc ttcggtatgc
     7141 aggcggataa acagcccaac gatcaacatc accgcagaaa gccaaaacgg aatgcgccat
     7201 ccccaggaga gaaactgctc cgaagtaaga aaatggttac agagagcaaa aataccggtt
     7261 gccagcataa tacccacaga cgcagccgtt tgcgggagtg aacctaaaaa tccgcgtcgg
     7321 gattcgggcg ctgattctat ggtcatcaat gccgctccgc cgtactcgcc accaagacca
     7381 aagccctgaa ttaaacgtag caccattaat atgagcggtg cccagatccc aatttcctga
     7441 tacgtcggaa taaaaccaat caaaaatgtg gagcaaccga caatagccaa cgtccagata
     7501 agcgttattt ttcgccctat tttatcgcca aaatgaccaa agacgatgcc acctaaagga
     7561 cgagtcagaa aaccgacacc aaaggtcgca aaggaagcta taagacctat aaaaggatcg
     7621 ctgtctggat aaaacagcgg tgcaaatacc agtcctgctg ccgtgccaaa aataaagaag
     7681 tcataccatt ccattaacgc gcctgcgaaa ctgcccataa cgacacgtct catggcttta
     7741 ccggaaagcc cttcacgcgg tgcgggtaac ttttcagaca taattttcct ctcctggtag
     7801 gcgagaaatc tgggatacga accactaaac gtgggcagtt tgaattaatg cgcatcggct
     7861 taaaccgatg cgcattaaat tatttcttca attcagccag aacctctgcg tcctcagcat
     7921 caacatttgc acccagtggt tcccaattaa tcaatacaca ggtcaggaat cccaggaatg
     7981 gcacgattgc caaccagtaa aacgcttcgg ttttcaacgc accccacagc atcggccaga
     8041 aaatgagtcc tgcaattgcc cctgtgcgca taatagcgcg ggcaaatccg accccagtag
     8101 ggcgaatagc gggaggataa gagagtgtgg caatggtcat gcccagccca ccaggacccg
     8161 ctgaatggaa gaaaataatt gatcccaata accctaaaga cagccaggga ttagtcgttg
     8221 ccagtgcgcc taacgacagc aatgctaagg tcacaacagc aaatccatac atcgactgtc
     8281 ggcgagtacc cagtcgttgg agaataaacg agccgatcat cccgcctgtc accccacaaa
     8341 gggcattcac gacggcagaa ccggtcaacg cgccagtaag cccgccactt attattcccg
     8401 ccaacgtaag aggaagataa acacctacgg cgttatattg ccacgcctgc atggttgcca
     8461 cgacacagcc aagaatggtg cgtttgcggt aacgatcatt aaataagatg ccatatccac
     8521 cactgtattt attctctgct ttttccgaga atttcgcttc tgttgtaccc gatgccgcaa
     8581 cgcgagcctg aacaccataa cgttttccca gaatgtcgca cgcttcctgg taacgcccga
     8641 cgcgtgcggc ccacattgcg gattcaccaa taaagaagta acgcaaaatc atgaagatga
     8701 ctgcaaaaat agctccgatg aatattccgt aacgccacaa ttgttcttca gcgatacccg
     8761 acagaagcag cggcaaaagc accaaataaa caacggttgt cgaaacatac caggccattt
     8821 gccagaggtt gaccgacgtt ccggttttct tcgacgttga accacgaagc tctgccacgg
     8881 cattggttgc caaagggaag tcgatgccta aaccaaagcc catgatgacg cggcacacca
     8941 gcacccacca gatactggga gcaatagcta ccgctgcagc accgatggtg cacagcccca
     9001 tgccaataat aaatgcccgc ttttgcccaa agcggtttat tactcggttc gctaataaac
     9061 cgccaattaa cgcggcgatt aatactgatg cattgacggt tgcagcaaga cccggcgaaa
     9121 tgccgaattg agcggcaatg tatttattac caaaccccac catcgccgcc tggtaggcat
     9181 caatcaatat gccgcccaaa gcaataaaaa taacgccgat actggtgttg attgcagagt
     9241 tcttattaac aatatcaatt acgtcctgtg gccgactaat tgaataagtt gcaggactgg
     9301 tgatttgact catcgcagtg tcctttttaa ttgttgtagg gtggcgctga cgtgacgcag
     9361 cgccagacag agatttagaa aatcattccg gcgcaaattg cagcatgacc tttatggagt
     9421 cattttgctg cgcgcgttcg aatgccgccg ctgcatcctc aatggcaaat aaatcggtaa
     9481 ccagttcact ggtatcaaac cgcccatccg ccagccattc gataacagca tcgaaatctt
     9541 cagggatgta catgccagag ttgagcaagt cacgctcgaa acgttgcatc atcggtaatg
     9601 gtatttcacg cggcccactt ggtacgccca tgcacacgac ggtgcctccg gcatatacac
     9661 gggtacatgc atcgttaagc gtggcctgcg ctgcaacgac atcaatcgcc cccgtaaatc
     9721 gcacatctgc ggccagctca cctggtgccc aaacttctgc tgcgcccagt tttaacgcca
     9781 gcgcgcgttt ggcagcgtca ggttcaatca cggtaatctt acctgccccc ataatgcgca
     9841 gtgcctgcac gatggataag cctatcgttc cggcaccaat caccagtacg ctttccagag
     9901 aagctttcgg cattcggtta acgcagtgac gagcacaagc cgccggttcg gcaaaggcca
     9961 acacttttag cggtaaagag gctggtgcga catggcaatt acgcgcagga acaatgtgtt
    10021 gtgagcgagc aaagcccggt gcgcgaaaac cagcaacctg cggtggttca caaagattaa
    10081 aacgtcctgc tttgcaggct cggcattcca tgcaagccat gatgggatcg accacaacat
    10141 gatcgcccgg ctgtacattt ttaacgtcgc tgcccacttc cgtaacgcgc gctgcaattt
    10201 catgaccggg gacgacaggc ggtttggcaa acggatgacc gcctttcaga acatgcagat
    10261 ccgaaccgca tatcccgtag aacacgggcg caatccgtac ttcgtgcggt ttaagttgct
    10321 ggtgtggtac ttcaacatat tgagtggtga ctttcccaat atctgaaaat aaaacctggg
    10381 taattttatc cattgttatt ctccagaatc gatatccata ggggattagc gttgcgcgat
    10441 accttcatcg acaatttcag ccagccgttg caagcgtgga acggagacat cgcgcagcat
    10501 ggtttgtgga tcttgtgcac caacaaccga cgcccaaacc gctcgtccag ccaggaagcc
    10561 cgacgcacca cctttcatgg caatacttac tgcacggcca aaaatatcgg catcaacgcc
    10621 agaagaaaga attacccacg gcatcttcat ctgatcgtta agttgctgac aggcagcaag
    10681 taaggttttt tcatcgcctt tgccccctaa tggcatctcg gctttgtaaa gatcagcctc
    10741 tgttccacca agctctgcgg cggcggcaac aatggcgctt tcacgatcaa agtcccaacc
    10801 gcgacgaggt ggacgcacca caggttcaat aatgctaacc agcccggcac tacggcagcg
    10861 acgaacaaat ttatcaacca ttgccagacg ttcctctgcg ggttcatctt cacgccagag
    10921 tacaagtaat ttcattgcct tagcccccat ttcacgggct ttatgcgggt cgacggacat
    10981 atctatttca acgctatcaa ccgggatgcc attacctggg ataaaaagat cggcagcgac
    11041 aatcaggccg caagaattag ccacagcgcc ctgctcgact atttgttcaa gacaaaattg
    11101 tttatccgcc agaacggcag aagcataagg agaaaggatt cgggtcgcgg caactttaaa
    11161 atcggtgagt acactgtcgg cgatgggttt tggctgccct gctgccgcaa acatcaggcg
    11221 catggcctca cgctgatcga cagctaacat ggcgaatcca ccacctgggc gggagatatc
    11281 tttcagcgta aatggggtga agtgggatga catagaaact cctgttaata gataaaatta
    11341 aagaccagcc tgctggcgaa cctgatttag cagggcgctc cagtcctgcc gccctttgcc
    11401 gtcttgactt gctgcggcgt aataactttc tgctgcaatc cccatattga gcggcgcccc
    11461 cgttttccga gcgacgtcaa ctgcaatacg cagatctttc aatgccaggt ccaccatgaa
    11521 ggcgggagaa agatcgcctt tcagcacttt gccaggccag gtggtcgtaa aatgtccttt
    11581 ccctgctgcc gtaccactca tcaccttaat tgcgacatca agatttaatc ccagtgactc
    11641 acataacaca gcggcttcgg aggacagcgc attaagcgca atgctcatat agttgttaat
    11701 tagcttcacg cggatcccca ttcctgggcc accagcctcg accagctcat tacccatgca
    11761 catgagaatc gggcgtgcac gcgcaatctg ctcatcagtg ccgcccgcta aaatcagcaa
    11821 tgtgccatca atcgcattca ctgacgttct gcccacgggg gcgtccatca tattgatgcc
    11881 tttttcttgt aattcccgta ttaatgcatc ggtctgtaag ggatgaatag tcgacatatc
    11941 aatcactaac gcgtcagatg aaacagtttc acagacgcct ttttccccca acagcacctg
    12001 tcgaacaatg tccccattgg gaagcatagt gatgataaat tcagcgcttg ctgccgcttc
    12061 cgctggggtt tgtgccgcag tggccccctg cgtaactagc gcatcgacgg cctgtgtatt
    12121 gacatcaaat acctgtaatg aatgcccttt ttgcaataga ttgttagcca ttgggctacc
    12181 catctgcccc agcccgagaa atgcaatggt tgccatgaat actctcctgt ctgacattga
    12241 caaaataatg acaattgaaa attcgttttc gtctgttttt gagcatattt gtaatttatg
    12301 ttcaaaaaca cgacacgcat cactaatttg atcatttgtg tcatttaaat tgaccaaaga
    12361 cgacaaaatt aaacaggaca agacaaatat gaccagaatt gcatgcgtgg gtatcacagt
    12421 tcaggacaga atttattctt tgcccactct gccggagggt ggcgggaaat atcaggcaaa
    12481 tcattatctt gagattggtg gcggcccggc agcaacagcc gctgttgcaa tcgcaaagct
    12541 tggcgtggag gttgatttca tcggtcgcgt cggcgatgac agttgtggaa ataccttact
    12601 tgcagaactt gagggttggg gcgtcaatac cgcattttgc cgccgatatc ctaacgcccg
    12661 ttcgtcgcaa tcagcgatcc tcgttgatca gcatggcgaa cggattatcg ttaattatcc
    12721 aagtcctgat ctcggtactg atgccgaatg gttagaagcg atcgatttct ctcgttatga
    12781 tctgattctg gccgatgttc gctggcatag cggtactgaa aaagcattct cgctagcgcg
    12841 ccttgcgggt gtgacaacgc ttttagacgc cgatatgaca ccgcaggata tttctccact
    12901 tgtcgcgctt gccgatcacg ccgtgttctc aacaccaggg ctgaaacgca tgactggcct
    12961 gcagtcacct gaagaaggtt tgttccaggc aacgacacaa acagcaggta aagtgtatgt
    13021 tacgctgggt tctgaaggat cgttgtggat tgaagatggt catttgtgtc aacaggaggc
    13081 attttctgtc aatgtggttg ataccactgg agcaggcgat gtttttcacg gcgcactcgc
    13141 ggtagcgttg gcagaaaaaa tgccaacaaa agaggctatt cgttttgcca gtgcggttgc
    13201 cgcgatgaaa tgtacccagc ctggcggtcg ggccggcatt cctaatcgtg agcaaaccga
    13261 atcatttttg tcactttatg cgtaaaatgg taaacatcta ccgaggaggg gcaagggagt
    13321 attcatgagt atcatcgaag tgacaggtaa tccacgccat gaccaactcg ttcatctaat
    13381 cgcagaacgc ggttatatga atattgaaga actggcacaa ctgctggatg tttcaacgca
    13441 aaccgtgcgc cgggatatcc gaaaactcag tgagcagggc ttgatcaccc gtcatcatgg
    13501 aggggccggg cgcgtctcca gcgtcatgaa taccgctttt gagcaacggg aactttcgct
    13561 caccgccgag aaacgggcga tcgctgaggc agtcgccgat taccttcccg aacgctgtac
    13621 cgtctttatc accatcggta caaccgtaga agccgttgcc agggcattac tcaaccggcg
    13681 tgatttacgc attatcacta atagcctgcg tgtggcacag attctttata agaatcagga
    13741 tattgaagtg atggtgccgg gaggcacttt acgcgctcat aacggtggga ttatcggccc
    13801 aggagccgtg gattttattg aaggcttccg cgcagattat ttaatcacca gtataggtgc
    13861 cattgaacat gacggcaccc tactggaatt tgatgtcaat gaagcgttag tcgcgagaac
    13921 gatgattaaa catgcgcgga atacattgtt agtggccgat catacgaagt ttaccgcgtc
    13981 tgctgccgtt tcaattggca atgcacggaa tgtcagggct ttttttactg atgccccgcc
    14041 tcccaattct ttctgccagt tgttaagtga agagaatgtt gaactggtgg ttgccgagca
    14101 agaagtatcc tgaaattgaa acttcggaaa atccgctggg aatttcagcg gattttgttt
    14161 tccataagcc aaaaaaaaac ctgccatcgc tggcaggttt tttatgactg aaaaacgaaa
    14221 ttaatcgtct ttcggtgcgc ggttagcgcg acgacgatcg ttttccgtca ggtgacgttt
    14281 acgaatacgg atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag
    14341 agcttgttcc agagtcatgc ggataggcgg aaccagaaca acggcttcgt cagtaccgga
    14401 agcacgcatg ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg
    14461 gctgtgaata ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt
    14521 accgcggtcc tgcagaccga acagtgcgaa cgcgaccgct ttaccctgac cgttagagat
    14581 cagtacgccg ttctgacgct ggcccacttc acccggacgt acgtcgtcgt agtggctgaa
    14641 ggtggagtac agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat
    14701 cagaccgcgg cttgggatca cgtagtcgag acgtacacgg cctttaccgt ctggattcat
    14761 gtttttcagg tcgcctttac gttcgcccag cgcctgcatt acagaaccct gatgctgttc
    14821 ttcaacgtcc agagtcacgt tttcatacgg ctcttgttta cgaccgtcga tttcacggaa
    14881 gataactttc ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgatcag
    14941 aacagacagg tgcagttcgc cacgaccaga aacgcggaac gcatcggcgt cttcggtttc
    15001 ttctacgcgc agcgcaacgt tgtgtaccag ttctttgttc agacgatcca ggatctgacg
    15061 agacgttacg aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat
    15121 agaaacggtc ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca
    15181 aacggtgtca gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc
    15241 cgccagatcg gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc
    15301 gttgcgggtt ttgccttcgc tatcgatgat agtaacctgt tggttcggct tcactttacc
    15361 gcgcttgatg cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat
    15421 ctgcatctgg aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc
    15481 ctggtacagc ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt
    15541 cagcgcagaa gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac
    15601 gaacagatcg aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt
    15661 gttgataaca acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt
    15721 ttgcggcatc gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat
    15781 tacacgttca acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg
    15841 gtaatcattc catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc
    15901 caaatcgttg gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga
    15961 ttgttggagc agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc
    16021 gatattacgc aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg
    16081 tacacggatt aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc
    16141 agagtccctt tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac
    16201 caatatggtg cttaatgttc ccattgaagc actatattgg tgcaacattc acatcatggt
    16261 gcagcccttt tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg
    16321 cacagttttc gctttatctt ttttacggcg acacggccca tttaattgca gatttcgtta
    16381 ccacgacgac aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg
    16441 atgctgaacg agcacgaagt gaagtttgtt gacttgcgct tcaccgatac caaaggtaaa
    16501 gaacagcacg tcactatccc tgctcatcag gtgaatgccg aattcttcga agaaggcaaa
    16561 atgtttgacg gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg
    16621 atgccagacg catccaccgc agtgattgac ccgttcttcg ccgattctac cctgattatc
    16681 cgttgcgaca tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt
    16741 gcgaagcgcg ctgaagatta cctgcgttct actggcattg ccgacaccgt actgttcggg
    16801 ccagaacctg aattcttcct gttcgatgac atccgtttcg gatcgtctat ctccggttcc
    16861 cacgttgcta tcgatgatat cgaaggtgca tggaactcct ccacccaata tgaaggtggt
    16921 aacaaaggtc accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactca
    16981 gctcaggata tccgttctga aatgtgcctg gtgatggaac agatgggtct ggtggttgaa
    17041 gctcatcacc acgaagtcgc taccgcaggt cagaacgaag tggctacccg cttcaatacc
    17101 atgaccaaaa aagctgacga aattcagatc tacaaatatg tcgtgcacaa cgtggcgcac
    17161 cgtttcggta agaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggttccggt
    17221 atgcactgcc acatgtcgct gtctaaaaac ggcgtcaacc tgtttgctgg cgacaaatac
    17281 gcaggtctgt ctgaacaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg
    17341 attaacgccc tggcaaaccc gaccaccaac tcttacaagc gtctggtccc gggctacgaa
    17401 gcaccggtaa tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg
    17461 gtttcttctc cgaaagcacg tcgtatcgaa gtacgtttcc cggacccggc ggctaacccg
    17521 tacctgtgct ttgctgccct gctgatggcc ggtcttgatg gcatcaagaa caagatccat
    17581 ccgggcgaag ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc
    17641 ccacaggttg caggctctct ggaagaagca ctgaacgaac tggatctgga tcgcgagttc
    17701 ctgaaagccg gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc
    17761 gaagaagatg accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc
    17821 gtctaatagt tgaagttgta ctacccggcg caacaacgcc gggatttagt tgccgtggaa
    17881 actttcagcc catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt
    17941 tttatccgta aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta
    18001 tggcaacagg cacgcagccc gatgctgggc agatccttaa ctcgctgatt aacagtattt
    18061 tgttaatcga tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa caactgctcg
    18121 cccaaagctc ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat
    18181 taaatatcga gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag
    18241 tgacgctggt catcgacggg cgctcgcata tcctttctgt gacggctcag cgtatgccgg
    18301 acggcatgat cctgctggag atggcaccga tggataacca gcgccgctta agtcaggaac
    18361 agctacagca cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga
    18421 ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa gcattacctg
    18481 atccgtcact actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc
    18541 tggtcgaccg tctgttgggg ccgcagctgc ccggtacgcg cattaccgaa agtattcaca
    18601 aagtggctga acgcgtggtg acgctggtgt cgatggaact gccgaacaac gtgcggttga
    18661 ttcgggatta cgaccccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg
    18721 tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg gccggagggt ggtgaaatca
    18781 ttctacgtac ccgcacagcg tttcaactga ccttacacgg cgagcgctat cggctggcgg
    18841 cgcggattga tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt
    18901 tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta
    18961 atttgattga tcagcattca ggcaaaattg aatttaccag ttggccaggt cataccgagt
    19021 tctcggttta cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg
    19081 ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg
    19141 tttaacctgt acgacatttg agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac
    19201 gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa
    19261 gcagattaaa cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac attccgatct
    19321 ggatgctgcc gtcagcgcct atcaacaagg ggcatttgat tatctgccca aaccgtttga
    19381 tatcgacgaa gccgtggcgc tggttgagcg cgccatcagt cattaccagg aacagcagca
    19441 gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggtgaag cgccagccat
    19501 gcaggacgtg ttccggatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa
    19561 cggcgaatcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg
    19621 agccaaagcg ccgtttatcg cgctcaatat ggcggctatc ccgaaggatt tgatcgaatc
    19681 cgaactgttt ggtcacgaga aaggcgcatt taccggcgcg aataccattc gtcaggggcg
    19741 ttttgaacag gctgatggcg gcacgttatt tctcgatgaa attggcgata tgccgctaga
    19801 tgtgcagacg cgtttgctgc gcgtgctggc agacggtcag ttttatcgcg ttggcggcta
    19861 tgcgccggtg aaagtagatg tgcggattat cgctgccact caccagaatc ttgaacagcg
    19921 ggtgcaggaa ggcaagttcc gtgaggatct gttccaccgc ctgaacgtca tccgcgttca
    19981 tctgccgccg ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca
    20041 ggttgccgcg cgagagctgg gcgtagaagc gaagttgctg catccggaaa ccgaagccgc
    20101 gctgacgcgt ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gtcgctggct
    20161 aacggtgatg gctgccgggc aggaagtgtt gattcaggat ttgcctggcg aactgtttga
    20221 atcaaacgta ccggaaagca cttcacacat gcagccggac agttgggcaa cgctgttagc
    20281 acagtgggca gacagagcgc tgcgttccgg tcatcaaaac ctgctttccg aagcacagcc
    20341 agagctggaa cggacgttac tgaccaccgc gctgcgacat acgcaggggc ataaacagga
    20401 agcggcgcgg ctacttggct ggggtcgcaa caccctgacg cgtaagttaa aagagctggg
    20461 gatggagtga ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt
    20521 ttgcgcttta ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat
    20581 tatgctggaa tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca
    20641 aactgctgtt gctgccgtgc tgtgtgccgc gatgataggg ctgtttagct gaagctcgtt
    20701 gtttacgccg gatgcggaga aagtcgtagg cctgataaga cgcaaccagc gtcgcatcag
    20761 gcgttgattg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa
    20821 cagattttgc aggcctgata agcgtagcgc atcaggcaat cttgttctca gcacattgat
    20881 ggcgctttcg ttttctactt tgtaaacgaa gcgccattca caatttaaat cacccgagag
    20941 aactgctgca tccgcgcttt ctggcgcaga taggtatcaa agcacatgca aatgttgcgg
    21001 atcagcaagc gacctttcgc cgtcacctgt attcccttct catccacatc caccagccca
    21061 tcttttgcta acggggcgag cagcttgaga tcttccgcaa agtaatcagc gaagagcaaa
    21121 tcccactgtt gctcaatagg ggcgtaatcc agacggaagt tgcagatgag cgacttaatc
    21181 acatcgcggc gaatacagtc atcacgcgtt agcgcaatac cacgccacag cgcattgcct
    21241 tgttcatcca cttgctgata gtactgcttc aactctttct ggttctgcgc gtagcagtcg
    21301 ccaatcatgc tgatggcgga aacgcccatc cccagcagat cggtatcgcc ctgagtggtg
    21361 tagccctgga agttacgatg cagcacgcct tcacgctggg ccaccgccag ctcgtcatcc
    21421 ggacgggcaa agtgatccat accgataaac tgatagcccg attgtgtcag gaaggcgatg
    21481 gtttcctgca ggatatcgag tttttgctgc ggactcggca ggtcagcatc tttgattttg
    21541 cgctgggcgg caaaaatggt cggcaaatgc gcgtagttaa agacgctcag acggtcaggg
    21601 ttcagttccg ccacacgctt cagggtaaag gcgaaactct ccggcgtctg tttcggcagg
    21661 ccgtaaatca ggtcaatgtt ggtggaggta aagccaatct cacgcgcatg gttaagcagt
    21721 gcaaagatga actcttcatc ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag
    21781 tcctgcacgc ccatgctcag ccgattaaag ccttcggcgc gtaaatgatc gagtacatcc
    21841 agttcgattt cccgcggatc gacttcgatc gagatctccg catcggcatt gaactggaag
    21901 ttttcgcgca gcagcttcat cagacggctg atttgcgctt tattcagata cgtcggcgtt
    21961 ccgccgcccc agtgcaattg gctgacgtga cgcccggcga acagcggtgc acgatggacg
    22021 atttcttgct ccagcgcgtc cagatactga tcggccttgt gctgctggcg agtaacaatc
    22081 ttattgcaac cgcagaagta acaaagctta tggcagaacg gaatatgtac atagagagat
    22141 aatggacgct caggatagcg cgccacagct tgtaaaaacg cctgttcgcc gaagtcttct
    22201 gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc cggaatagtt atatttctgg
    22261 atcagggcca gatcccagtc gatttgctgt acagacatgc tcactccttc cgatggcgtc
    22321 tctggcggct acggcgtacc ggcggatgcc cgtttcgagt catcagccgg ttgcgcagcc
    22381 acttttgtcg ccgcgacaac cgctgtagtt taacgaataa ccacaccaga taacatataa
    22441 ccggaagggt tatcagcaga acgggaaggc ccacggcggc aatccgttag ttgccccgca
    22501 gcagacgcat catgtcttct tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag
    22561 agaggccgag tttctgcatc aactcatcaa tacgatccag tttggcatcc acccaggatt
    22621 gctcttcggc gctcagggtt tcgcctgctt ccagacgttc cagcagcgca tccagacgct
    22681 catccgtttc cagtaactcc aactccgcct gcggtgaaag cataggtttc tcactcttcg
    22741 gtttgtgctg tttggtgact ttttcagtca cgcccaatgg aataggggtt ttactgccaa
    22801 tacgtggatc ttttggtgcg ttctgacctt tgctgcctga cgtggtgtta ccgcccgctg
    22861 cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg cttacggtca cgagcttcct
    22921 gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg acctttgctg cgtgagtttg
    22981 aagatgatgg tttcataatg gttcgtcttt tagccgtttt attcagtata gatttgcggc
    23041 ggaatctaac agaaagcaag caaagaaaaa aggcgacaga ttactctgtc gccttttttc
    23101 ctgactcata acccttaacg ggtcttacaa tccttgcagg ccgccaacac accctgtttt
    23161 tccattagcc ggaacaatcc gtgttgattc catttccatt taattacgtc tccggacgtt
    23221 tatcttcctg acaatcctct gtcttcgcct cctggcgctc ctgaccctca tcctgagtca
    23281 ttgttcctgt tagcgtcctc gctctatggg tgctacttta cgcctttgct ttaaataaac
    23341 aatcaaccgc catcagtatt tgcacatttt cagagtttta tataaaagca attcactgat
    23401 ttatatagct tatttgacat acgtcgcaga attttctctg ctatttcgca taaagactat
    23461 cggcaatgtc tcacaaagca tatggctttt acttacaacg ctcacgccga aaaatccgcc
    23521 ttttattatg gttcaggtat aatcgccgca aagctttgac tgatggagac gaccgctttg
    23581 actaatttga attatcaaca gacgcatttt gtgatgagtg cgcctgatat tcgccaccta
    23641 ccttccgata ccggaattga agtggctttt gcaggccgtt ccaacgcagg taaatccagc
    23701 gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac cccagggcgc
    23761 acccagctta tcaacctgtt tgaagtggct gacggcaagc gtctggttga cttgcctggg
    23821 tacggttatg cggaagtccc ggaagagatg aagcgcaaat ggcagcgcgc gctcggcgag
    23881 tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc taatggatat tcgccatccg
    23941 ctgaaagatt tggatcagca gatgattgag tgggcggtag acagcaatat cgccgtgctg
    24001 gtactgctga ccaaagcgga caaactggca agcggcgcac gtaaagcgca attgaatatg
    24061 gtgcgtgaag cggtgctggc gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgctg
    24121 aagaaacaag gcgtggacaa gctgcggcag aaactggata cctggtttag cgagatgcag
    24181 cctgtagaag aaacgcagga cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt
    24241 ggcgtatccg gcccgtaaat tcaatagatt acaattctac cgttggcctg ataaacaggg
    24301 cgcatcaggc attacggatc ttttctttcg cccaataaaa aacgccccag tcattactga
    24361 ctggggcggc taaaatattc agccaaatcc gattacgtga agtaaaaggt ctgaaagata
    24421 gaacatctta cctctgtacc ctacgcgact aactttactc ttttttgttc agttcagaaa
    24481 gcattttttg taattttttt tcagttgttg cataggaatt tctaatagcc atcacaaaac
    24541 gtgacagctt atgttgctta cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc
    24601 ctgatcccag ttttcgccac tccccacttc caccagcaac ggcacatcca gacgggtaca
    24661 gttttccatc agttgatgaa tctgcttcgc gacggcatcg acatcatctt tatggacttc
    24721 aaataccagt tcatcgtgta cctgcatgat catacgcaca cgcggttgct cagcctgtag
    24781 ccacgcatca acggcaatca tcgcccgttt gataatgtcg gcggcggttc cctgcattgg
    24841 cgcgttaatg gctgcacgtt cagccgctgc acgacgagcc ccattgctgg atttgatatc
    24901 cggcagatac agacggcgtc cgtccagcgt ttcaacgtag ccctgctctt tcgcctgagc
    24961 acgggtgcgt tccatatact ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta
    25021 cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc
    25081 ataaatcaga ccaaagttga tcgctttcgc gctacgacgt tgctcgctgg tgacggtttc
    25141 cagtggcaaa ccaaacactt ctgccgccgt tgcacggtgg atatcttttc cttccgcgaa
    25201 tgcggtcagc aagcctttgt cacgcgagag atgcgccata atgcgcagtt caatctgcga
    25261 gtagtccgct gagacaatca cataatcctc tggcgcaata aacgcctggc ggatacggcg
    25321 accttcttcg ttacgcaccg gaatgttttg caggttagga tcggttgacg ataaacgtcc
    25381 cgttgcggtt actgcctggt gataagaggt atgtacacgc ccggttttcg ggttgatcat
    25441 caacggcagc ttgtcggtgt aggtcgattt cagcttcgcc agaccacgat attccagaat
    25501 cacttttggc aacggatagt ccagcgccag ttcttccagt acctcttccg acgttgacgg
    25561 cgcgccaccc ggagttttct tcagcggttt aatgccctgt ttttcaaaca gaatggtttg
    25621 taactgcttg gtggaagaaa ggttaaattc ttcacctgca atttcatgcg ctttcttttc
    25681 cagctcagcc agacgcaagg tgagctcttc agagtgattg tgcagcactt tcggatcgat
    25741 cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc agcggcattt cgatattctc
    25801 gaagacgttc aacggccctt tgtgtttttg cagatccggc cacattttca gatgcaactg
    25861 caaggtgaca tctgcatctt cggcggcgta acgtccggct tcttcgaggg caatctggtt
    25921 aaaggtcagt tgattttttc ctttaccagc aatctcttca aaagtgatgg ttttgtgctt
    25981 caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg gcaacgctat tgagaatgta
    26041 ggactccagc atggtatcaa acgcaatccc acgcagctca atgccgtaat tcgccagaat
    26101 accgcgatcg tatttcaggt tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg
    26161 ttttagcaac tcgagtgcac gctcgcgaga gatttgatcg ggcgcatcaa gataatcatg
    26221 agcaaccgga atatatgccg ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt
    26281 agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc
    26341 cagcttcgca atccacgctt tcagtgtttc ttcatcaagg atggtgacgt agttgtcata
    26401 agaaatcacc gttgccgtca cttctggcgc ttcgtctgca acactggttt cctgcggcct
    26461 ggctgcgggt ttcgcccctt tagcctgtag ccatttgccc gcttcgacat cagcagtcca
    26521 gcgtttgaac tcatactttt tgaacagccc taacaactct tccgccgccg gtggctgcac
    26581 ttccagttgt tcacaggtca gctccagttc aacgtcggtt ttaatcgtcg ccagctggta
    26641 tgagagataa gcaacttctt tgttttgctc gagcttggct gccattgttt tcgcgccacg
    26701 gaagctcaac ccagcaattt tttctggctc ggcatacagc gtatccagcc cgccaagacc
    26761 ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt acgccaggga tgttatcgga
    26821 ggagtcaccc atcagcgcca ggaaatcgat gatcagttct ggcggcacgc cgtacttatt
    26881 caccacctct tccggtccga ggatggtatt cgtcatggtg ttgataagcg taatatttgg
    26941 cgtcaccagc tgcgccatat ctttatcgcc agtgctgatc agcaccggac gcccggcttt
    27001 ttcggcttcg cgcgccagag taccgataac gtcgtccgct tctacgccag aaaccgccag
    27061 cagcggcagt cccatcgctt taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc
    27121 gtccggcatt ggcgggcgat gtgatttgta atgttcaaac agttcatcac gaaaggtttt
    27181 tcccttggcg tcaaagacca ccgctgcatg cgtcggttta tattgcatga tcagactgcg
    27241 cagcatgttg aggacaccat acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg
    27301 gggaaacgcg tgatatgcgc gataaagata agatgaacca tctacaagga taagtggatt
    27361 ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag attatgggta aaggatgcca
    27421 cagacaggat gaaaacatga gtttttcacc gcttttcgtg aaaatctttt gtagatcttc
    27481 tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat aaattattta catcgctttt
    27541 aatttattat catgcttttt acagcatgat gtttgtcttt gattttcatg cagttaaaaa
    27601 taaagccatc ccttgagata atgggatttt ttctaatgtg gatagatatg aattattttt
    27661 ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc cttgcgcttc
    27721 tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag aatactcgct
    27781 gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt ttgctccgtc
    27841 ccgatcatca ttgccgggat tgtaaaacta ttgcttcctg tgccagtcat ttggcgaaag
    27901 gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc ggtattactg
    27961 catctgaacc cacacctgca atgggaagtt cgcgggctgg aagggttaag taagaaaaac
    28021 tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact gtgcgtgctg
    28081 tttcgtaagc acattccgat gaataaatat ttcctcaagc agcagctggc ctgggtgcct
    28141 tttcttggcc tggcatgctg ggcgctggat atgccgttta tgaagcgtta ttcccgcgct
    28201 tatttattac gtcatccgga gagacgtggt aaagatgttg aaaccactcg gcgttcttgt
    28261 gaaaagtttc gcctgcatcc caccaccatt gttaatttcg ttgaaggctc ccgtttcacg
    28321 caagaaaaac atcagcaaac gcactccact tttcaaaact tgttgccacc aaaggcggca
    28381 ggcattgcga tggcgctaaa tgtactgggt aaacaattcg ataaactgct taatgttacg
    28441 ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg taaactgacg
    28501 cgaattgtcg tacatgtgga tttgcagcct attgccgatg agttacatgg cgactacatc
    28561 aatgacaaaa gctttaagcg ccattttcag caatggctga actcactgtg gcaggaaaaa
    28621 gaccggcttc tgacgtcgtt gatgtcatca cagcggcagg acaagtaaat tttgctcgcc
    28681 tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca cgagacataa
    28741 attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta taacgcagtt
    28801 gcagcgaggc ggtattgttg tcaatcgtga ttaactgcaa catctgacct aaggtcatca
    28861 tcccgctgat ttgtttatca gactgctttt gcgctttttc cgcatccatt ccttcagata
    28921 aattaagctg ttttgcggtt tctgtggcga cattaagcgg taatttcaca tcaaaattga
    28981 gcgatttaat atctttgttt gtggatgatg aagatttggc tgggtcggca atactgatat
    29041 ccagattggc atttagttcg ccgagtgcat tcttccatct taccggttgt ttaatggtcg
    29101 gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagtattc acttcttgca
    29161 gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc atcgcaatgt
    29221 tatactggat gataaattgg cgcaccgctg agggatcaat agattcagca atcaatgacc
    29281 actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg taggtcagat
    29341 tgacattgat ataatctttg tctttaacgc gatccagctt ggtttttgca acgaaagcat
    29401 cgatttgggc aacgtcttcc ccgtgattga taatgttaat ctgattaaat ttcgactcgc
    29461 tttcaccaac cggaaatttc ttttcttcta atcgcgccag agaatcaatg gtaaaacttt
    29521 tcgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcaact ttgccttcaa
    29581 tatccaggtt agacaatgaa ctatcatcac cattaaaagt aaactggcct tcaccaaaag
    29641 aaaactttcc atactctgca ggggcaacat ctaatgtcgt ggagaatgag ccaccaaacg
    29701 cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat agcggttgtg
    29761 tcagctcatt attcactaag ttcacttttg ccgctgccag tgctgggatg acattaccat
    29821 gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat acaactttct
    29881 ggcctggctt gatattgaga tcgggtgcac cgttatcaaa tgtcatttgc atttgaaaac
    29941 gggaattaaa aaaacctttg tcaaaatttt tataacttaa cgtcatgtcg taggcatgtt
    30001 cacctttcta tttagcatcg ttaaaatctt taataaactt ttcgacacct ggctgaattt
    30061 gcgtacctgt gtaccatgtg ccaccacccc agattacgga taacgcaaca atggcacctg
    30121 tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt tgaatatcca
    30181 cttatgctga aataatgtcc agcagcagga cgcattatca gaatgtattt a
//
LOCUS       DAPQZD010000048        29363 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPQZD010000048 DAPQZD010000000
VERSION     DAPQZD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.048"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(53..1123)
                     /locus_tag="RO454_002054"
     CDS             complement(53..1123)
                     /locus_tag="RO454_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDY0933891.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPVTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            complement(1120..2025)
                     /locus_tag="RO454_002055"
     CDS             complement(1120..2025)
                     /locus_tag="RO454_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDY0933892.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAG"
     gene            complement(2022..4406)
                     /locus_tag="RO454_002056"
     CDS             complement(2022..4406)
                     /locus_tag="RO454_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDY0933893.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNEKRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDECQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSVGRLFVLVNKFDEKDRNGDGADAVCQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFLDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            complement(4624..5058)
                     /locus_tag="RO454_002057"
     CDS             complement(4624..5058)
                     /locus_tag="RO454_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933894.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            5487..7652
                     /locus_tag="RO454_002058"
     CDS             5487..7652
                     /locus_tag="RO454_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDY0933895.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVHDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSICHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            7663..8652
                     /locus_tag="RO454_002059"
     CDS             7663..8652
                     /locus_tag="RO454_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0933896.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            8671..9729
                     /locus_tag="RO454_002060"
     CDS             8671..9729
                     /locus_tag="RO454_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDY0933897.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            9726..10493
                     /locus_tag="RO454_002061"
     CDS             9726..10493
                     /locus_tag="RO454_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0933898.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPELLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            10547..10804
                     /locus_tag="RO454_002062"
     CDS             10547..10804
                     /locus_tag="RO454_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933899.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(11335..12480)
                     /locus_tag="RO454_002063"
     CDS             complement(11335..12480)
                     /locus_tag="RO454_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933900.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTKWLKKEPRASYF
                     FLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPHPIPEMD
                     SSIVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAM
                     GFPSQPKIAGPREKPMRTVHPDNIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(13607..13762)
                     /locus_tag="RO454_002064"
                     /pseudo
     CDS             complement(13607..13762)
                     /locus_tag="RO454_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(13806..13916)
                     /locus_tag="RO454_002065"
                     /pseudo
     CDS             complement(13806..13916)
                     /locus_tag="RO454_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            14005..15027
                     /gene="istA"
                     /locus_tag="RO454_002066"
     CDS             14005..15027
                     /gene="istA"
                     /locus_tag="RO454_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDY0933901.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETELGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            15027..15719
                     /locus_tag="RO454_002067"
     CDS             15027..15719
                     /locus_tag="RO454_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0933902.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQK
                     RKAGVIAEANPE"
     gene            complement(15786..15914)
                     /locus_tag="RO454_002068"
                     /pseudo
     CDS             complement(15786..15914)
                     /locus_tag="RO454_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(16745..17347)
                     /locus_tag="RO454_002069"
     CDS             complement(16745..17347)
                     /locus_tag="RO454_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933903.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(17441..17647)
                     /locus_tag="RO454_002070"
     CDS             complement(17441..17647)
                     /locus_tag="RO454_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDY0933904.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(17843..19039)
                     /locus_tag="RO454_002071"
     CDS             complement(17843..19039)
                     /locus_tag="RO454_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HDY0933905.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            19839..19979
                     /locus_tag="RO454_002072"
     CDS             19839..19979
                     /locus_tag="RO454_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HDY0933906.1"
                     /translation="MNSHTRYKLTLAELEAFNSAVDNRLAELTANFTSACWLQSCHLS
                     VR"
     gene            20619..21188
                     /locus_tag="RO454_002073"
     CDS             20619..21188
                     /locus_tag="RO454_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDY0933907.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            21354..21755
                     /locus_tag="RO454_002074"
     CDS             21354..21755
                     /locus_tag="RO454_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933908.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            21743..22153
                     /locus_tag="RO454_002075"
     CDS             21743..22153
                     /locus_tag="RO454_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDY0933909.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPSCGTGVYSREGGLQEGNWKDRN"
     gene            22437..24056
                     /locus_tag="RO454_002076"
                     /pseudo
     CDS             22437..24056
                     /locus_tag="RO454_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            24136..24428
                     /locus_tag="RO454_002077"
                     /pseudo
     CDS             24136..24428
                     /locus_tag="RO454_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(24468..25502)
                     /locus_tag="RO454_002078"
     CDS             complement(24468..25502)
                     /locus_tag="RO454_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDY0933910.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(25505..26470)
                     /locus_tag="RO454_002079"
     CDS             complement(25505..26470)
                     /locus_tag="RO454_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDY0933911.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(26524..27282)
                     /locus_tag="RO454_002080"
     CDS             complement(26524..27282)
                     /locus_tag="RO454_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDY0933912.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(27296..28510)
                     /locus_tag="RO454_002081"
     CDS             complement(27296..28510)
                     /locus_tag="RO454_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDY0933913.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(28706..29044)
                     /locus_tag="RO454_002082"
                     /pseudo
     CDS             complement(28706..29044)
                     /locus_tag="RO454_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7070 a         7373 c         7570 g         7350 t
ORIGIN      
        1 caccagccag gaaaaacacc accacaggcg gtggcgtttc tttggggcgt ctttatttaa
       61 tccgggccat cgcgtttctc agtgccaaat ctgcctcttc cgttatctcc agacttttct
      121 gcaacaccgt ttctgaccgg gacagtacct gacgtgctgc cagcggtttc cggatatgct
      181 ccatcgtcag catcgtgtct tcatagccct gctgacgcca ggcgtcggta tgtgccaacg
      241 tgaaactgag cagaccgccg ctgttgtcac catcacctgc atatttcagc cttttccggg
      301 gacggatctc aaggattgtg gtgaccggaa aagcctggcg atcccataaa gaaccgtcac
      361 tcagatgcgt cacaatcacc atattgcatc cggcatcgac cagaggcgtc acaggggtat
      421 ttccctgcat atttcgccat cctcccatac caccatcacc gaacattgtc ccctgaacct
      481 cacggggacg gaacagcagg ggcagcgcgg ctgacgcaag cagagcctct ttctgctgtc
      541 cgcggggcag gctctggata tgctgaaaaa cggcgttttt cgtggttccg gcacccagtt
      601 cagcacgaat gcagtcaata atatcctgca tgcccccttc tgtggggtac agcgacacat
      661 acagcggtag cccgtctgcc agagcatcag tatcaagata atgatccaac agcgctgtca
      721 ggggctcatc tgacaacaac ggctgagcca tcagattgtc aaacccgttg agtatcgata
      781 tatggcgaag aagcgtcgta agcagtgctc ctgcacgtcc ggggatctgg cagaggttca
      841 ttgcctggaa caattttttc agcaatgaaa agtaaaccaa tcggtttacc gacagcacct
      901 gattattccc cagatgatcc cagagcgcct ccaggcgtac ggcagcttct gacagatcgg
      961 gagaggccgc gataatggca ccattgaatg cgccaatgct ggcccctgaa accatgctga
     1021 tctgtgtacc acactctgcc agtgccttaa ccactcccgc ctgataagcg cccaccgccc
     1081 cgccaccgga cagcaccagc cccgttttca tttctgtact catccagccc ccttctgaaa
     1141 tatttcatcg accatacttt cagagaggcg ttctgcagag cagaggcgag gcatgcgaac
     1201 cggtaagtgt tctgcagcct gcgtggttga ccagatcacc ggatgctgct cttcactgca
     1261 ccagtctccc agccagttaa tcgcgtcatg ccactcgtta tctgcctgct cccggccctg
     1321 accaccaagc cagtccagag tgttcagacg cacggttgca ctaccgccac tctcctgtgc
     1381 catctgcgtt aatgctttat gtctgagaat atccagtagc tggttaacct ggcctgtttc
     1441 gccctgacgt atcacatcac caaacgcgcc ccagcgaagc tcttcaagcg ccacataaca
     1501 acgtccggca ggggaaaatg acgcatatcg cccggcgctc caccatgaga atatctgctc
     1561 aagatgtaac tgcgcccgct gaacctggtc aacacggtgg agtttttctt caagatgatt
     1621 cagtttatgg tgaacctgat cggccaggat gagtaactgc tcccgtgtat ctgcggaata
     1681 atgcgccagc ctggcagtat cactgaccag tgaactgacc ctgtcgttta cccgggtaat
     1741 cgcgtagttt gtggtggcaa gtgacgttgt cagctccgtc agccagcgca gggacgcctc
     1801 aaggccctgt gcaaccgaag cattgatggc attctgccgt gcagcgcctt cgccggtcag
     1861 tccctctttt aaacgccgga agaacgtgcg ctctccggcc gtgcggatat gctcctgtgc
     1921 aacatcgatt ccgtttgcga aatcaaccac aaatttttca ggaagacagg tgagtacctg
     1981 acatacccgg ttatcagcaa tttgttgaat aaatggtgaa gtcacagcat tgtctccagc
     2041 tcttccctca gcgccttact gtcagtaatg agctcaggaa ccctttcatt taatgcctga
     2101 agtctcctgg taagccgttc ctgtgcctgc tgatcgcgtt tatgatcttc agagctctga
     2161 atcagcgtgt tacgcagttg ttcaactttc tccctgaatg cgcagaaaaa ggcatcgatt
     2221 tcctggcgga cgggctggtt aatatcctgc tcaatggatg cctgcagctc cccgaaatat
     2281 gcccgggtca gtgacataac ttccttcctg acggtgttga tattgataac actgcggctc
     2341 acatcctctt tataggtttc ccagccccag tcactggtgc caaacgctcc acaaatcttt
     2401 ccccataaac cactctgccg acggcgacgg gtgacggtct ccgttctttc ctccataagc
     2461 gcactcagat ttgtttttac cgcgagccgg gtttgtaact gactgactgc cggaaaactg
     2521 atttttacgg taaagccctc atcctccagg cgggtattga tgcgaccggc aatgttttct
     2581 gtggcgtaca gggccgtgcc acgaaaacct gtttcaatcc cgctgacgat tttctccagc
     2641 tcaggccgga actgtgcttc agcctcacgg tgcaggctgg tcacggtcga ttcgatttgc
     2701 gtcatcagtt caagggcttc cctgcgatca ctgaatttta tttcggggct gtcgggatca
     2761 aagtcccgca tcctgtcatg ctgatttccg gttccgaaaa tatcgagaaa gaaagcgaat
     2821 gcgtttctct cccggggctg cgctgaattt tcttcctcca gcatttgctg ttctttgcgt
     2881 ttaccgcttc tgaaatagtc attcagctcc tcttccagtg aggtgcagac ccccgtcagt
     2941 aattcccggg ttctggcatc gatctcctcc atggccattc tgacatcagc ggtcacctgc
     3001 tcctgacagt ctgctatttc ccggatatcc tcaagcaggc tggtgatatg cgcctgcagt
     3061 gactgaatac tttgcatcag cccctgatgg cgtaacgaca ggtattcact gatattttct
     3121 gcattctgca tcagttttgc ggcggcagaa tcaaccgcca gtgccgcagc tctggagtgt
     3181 gaactcagta tgacttccgt tatcagttga tcgataagtg agccttccca caggtctgtc
     3241 gccccttcag ttgccagttc gctgtcttta cagacgtaat cttttttcat gcgaccgaag
     3301 gcttccctga caaaatcatc gacccaggct tcatcaacag gaagggttcc gtttttccgt
     3361 aactcatgaa gcgcacggtt agccaggtat gcctggcgtg aggatccggg ataaacgcgg
     3421 gaggcgggca gcacatcgct gttcagcatt gccggtacct tctgacagac tgcatcagcc
     3481 ccatcgccat tacggtcttt ttcatcaaat ttattgacca gcacaaacag gcggccgaca
     3541 gacacatcag caatggcatt aagctcttta cgaacctcct catcggcttt cgagttcatc
     3601 tgggtgtagt ccatcaccgc cagcaccgca gaggctttct gcagttgatc ccgcatcatc
     3661 acttccatct gcggttgtcc ggcttcatta gggccggggg tatccagcag tgtgagtgtt
     3721 ccctggcatt catccatccc caccagatgg ctgaattcca cgtcgatcac cggcagtttc
     3781 tgcacttctg cgtattcatc aaaaggaaat tcactcccca ttgccgcagc aagacgaacc
     3841 agatcgttta acgatgccag tccggtaaag atttcctcct ccccgtggta ttcatttttg
     3901 agccagccat catccgtcag aatatccagc agttcgcggg tatccccggt ttgctgcagg
     3961 gtctgtgcga cctgctgtcc tgccggggtg gcgagttttt cctgcagtgt gattaataaa
     4021 ttgcggacag gctgaatatg ttccagatgg agaaccggct cagtttttcc gggaacgtgg
     4081 cggatgagcg tcggtacaga ggtcatgggg cggttacggt taggcagaat ttcctgcccc
     4141 acaatggcgt tgatggtggt tgatttacct gctttcatcg tgccgacaac agccagcacc
     4201 atctccctgc gggcggtttt gatttgttcg ccctccagct cctcaattct tttcagtgct
     4261 ttctgtttat caaaaagcag tcttttttcg ttcttactgt cagataacac atctggctcc
     4321 tcaaccattt gccggagcag attaatgttc agttgcaaaa gtcggtcggc ttcatcacaa
     4381 agcagggcga tgttcttttc gtgcattatt taaccttcct gagacgcatt ttcatgacaa
     4441 ccacggagga ggtcatttac cacaaacgat cgtttataac gatcgatatg atagtaacga
     4501 tcgatgtatt ctattaactt gaaaaacaca acgcaagggg acaccccggg tatggctgcg
     4561 tttgcatagt gttttcatgg agttaaaaat ctgtaacaac agcctggtac atcaccaccg
     4621 ggttcagaag aaaatccagt tccataccgc gcgggcaaca gagaccaccg agtcacggac
     4681 ggcgcgcaga accgtgcgtg caacagaggc aatgcagaca ctctccgccg tgtcaaatat
     4741 ccggtccacc gcaccggtaa actgttcacg ggtctgagtg cggacagact ccgtgcgcag
     4801 ttcgtcctgc aggcgtgtca tcagagggat ggcggcatgt ccgggaagca ctgtcatgag
     4861 tgcactgacc ggggtgacct ccccctgaaa cagtgcatta cagagtgagg atttaccggc
     4921 cccgctttta cccataatgc caggcacggg ttcgtgacgg gtgagtttgc gcagatgttc
     4981 caggatgtga cgggaaatcg ggtaaggcag ggaggagagc ggtttttcaa ttgcctcaat
     5041 ggcatcagac ggattcatac atgtattccc agtgaaaaaa gacaaaaccc cgtgcaccgg
     5101 aaaggtggcg ggggattcac ggagataata tctttctgaa attattttga atgcaggtat
     5161 atgtggctga tttgtatcag ctaatgagta caggtctgaa tctaatctga cagtccgctt
     5221 tgtgccaaag gcggacgctc aagtagatac tgtcctgaca gtatgcggta atcagagttg
     5281 ttatatatta cttgcgtcca aaccagcttt ctgacaaccc ggccgctcct gctttcaaca
     5341 acgcgccgcc atacttttat gctgcaaaga gcggagacag aaggttagcg taatttgtaa
     5401 ataacttaca ttcacgtgtg atctggatca taattttgcc tatgcaccga tcgtaatata
     5461 aatatttaca taacgatcag tgattcatga aaactcaaat aactttcgct gcgcttttgc
     5521 cagcattagc gtctttcata ccgcttcatg ctcatgcctc gtctacttct gaagatgaaa
     5581 tgattgtcac gggcaacacc gccgccgaca ccaccgattc tgccgccggt gccggtttca
     5641 aaacgaacga tatagatgtc ggcccgctgg gaacgaaatc ctggatcgaa acaccatatt
     5701 ccagcaccac tgttactaaa gagatgattg aaaatcagca ggcgcaaagc gtcagcgaga
     5761 tgctgaaata ctctcccagt acgcaaatgc aggcgcgcgg tggaatggat gtcgggcgtc
     5821 cgcaaagtcg ggggatgcag ggcagcgtgg tggccaacag ccgtctggac gggctgaata
     5881 tcgtttcaac aaccgcgttt ccggtggaaa tgcttgagcg catggatgtg cttaacagtt
     5941 tgaccggcgc gctgtacggc ccggcgagcc cagcagggca gtttaatttc gtggcgaagc
     6001 gcccaaccga agagacgctg cgtaaagtga cgctgggcta tcaaagccgc agtgcgttta
     6061 ccggccatgc cgatctgggt ggccattttg atgaaaacaa acggtttggc tatcgcgtga
     6121 acctgcttga tcaggaaggg gaaggcaatg tgcatgacag cacgctgcgt cgcaaactcg
     6181 tttccgttgc gctcgactgg aatattcagc cgggcactca gctacagctc gacgccagcc
     6241 attacgaatt tatccagaaa ggctatgtcg gtagctttaa ctatgggccg aacgtcaaac
     6301 tgccgtctgc gccgaatccg aaggacaaaa atctggcgct cagcactgcg ggcaacgacc
     6361 tcactaccga taccatcagc actcgcctga tccactactt taacgacgac tggtccatga
     6421 acgctggcgt gggctggcag caggctgacc gcgcgatgcg tagtgtttcc agtaaaatac
     6481 tcaacaatca gggcgatatc tctcgttcga tgaaggattc caccgctgcc ggacgttttc
     6541 gcgtcctgag caacaccgcc gggctgaatg gtcatattga taccggctct atctgccacg
     6601 atctgtcact ttctaccacg ggatatgtct ggtcgcttta tagtgccaaa ggaacaggtt
     6661 ccagctatag ctggggtaca acaaatatgt atcacccgga tgcgatagat gagcagggcg
     6721 atggcaaaat ccgcaccggc gggccgcgat accgctccag cgtaaatact cagcagagcg
     6781 ttacgctcgg cgatacggtg acatttacgc cgcagtggtc ggcaatgttc tatctcagcc
     6841 agagctggct gcagactaaa aactacgata agcacggtaa tcaaacgaac caggttgatg
     6901 aaaatggttt aagtccgaac gccgcgctga tgtataaaat tacccctaac acaatggcct
     6961 acgttagcta tgccgattcg ctggagcagg gcggtaccgc accgacggat gagagcgtaa
     7021 aaaatgccgg tcaaacgcta aacccgtatc gcagcaagca gtatgaagtg gggctaaaat
     7081 cggacatcgg cgagatgaat ctaggcgccg cgctgttccg actggaacgt ccgtttgcct
     7141 atcttgatac ggataacgtg tataaagagc agggtaacca ggttaacaac ggccttgagt
     7201 taaccgctgc cgggaatgtg tggcaggggc tgaatattta cagcggcgtg accttcctcg
     7261 acccgaaact gaaagatacg gcgaatgcct caaccagcaa taaacaggtt gtcggcgtgc
     7321 cgaaagtgca ggccaatctg ttggcggaat acagtttgcc gtccataccg gaatgggttt
     7381 acagcgctaa cgtccattat acgggcaaac gcgcggcgaa cgataccaac acctcttacg
     7441 ccagcagcta taccacatgg gatttgggaa cgcgttacac cacgaaagtg agcaacgtcc
     7501 caaccacttt ccgcgtggtg gtaaacaacg tgtttgataa acattactgg gcttctatct
     7561 tcccatcggg taccgatggc gataacggtt ccccaagtgc gtttatcggc ggcggccgcg
     7621 aagtgcgtgc atccgtcacc ttcgatttct gatggataaa tgatgaaact ctcccgttta
     7681 tttccgctgc tggcgctgct gctggcggcc agcctgcacg cagaaaatac gcacaaggag
     7741 gtgcgtatcg cgtcaccgtg gccggcgcaa aataccatta ttgccatgct ggggtacggc
     7801 gataacattg tcggaacctc gatgattgca aaaaggatcc ccctgtttcg ccagagcctg
     7861 ccgcgcatag agaaagtcgc agcagttagc gtaaacagcg ggcatgaaat caatcccgaa
     7921 cagattatcg cgctgggggt cgatatgctt tttgtaccgc agaatatggt agtgcctcaa
     7981 caggcgttgc tgaaacaggc tggggtgcag gtgctggcgt ttgaggcgaa ttcactgcgg
     8041 gcgttaacgc agcgcgtaca gcaaacggca gcggtgctgg ggcccgatgc gcagcaaaaa
     8101 gcgctcgcct atcaacgcta cttcgatcgc aacgtggccc tggtcaccgg gcgcctgaaa
     8161 gatcttcctg cgtcacagcg cgtgtcgctc tatcacagta tgggcaatcc gctgaccacc
     8221 accggtagac cctcgctaaa ccaggactgg attgacctgg ccggcggcaa aaatatcgct
     8281 gaaaattggt ttggcgagca tcaacaaaac cgttcgggtg aagtcgcgct ggagaaaatt
     8341 gtcactgcca atccggcggt tatcatcgcg atgaacaagc gcgacgcgga tgccattctg
     8401 agttctccgc agtgggcaag cgtggacgcg gtcattcatc atcgcgttta cgttaatccg
     8461 aaaggaatgt tctggtggtg ccgtgaaacc agcgaagagg cgctgcaatt tttgtggctg
     8521 gcgaaaacgc tttatcctgc gcgatttgct gatgtggata tacgcaaaga gacgcgtgag
     8581 ttttatcggc agttctttgg cctgacgtta agcgatgcgc agatgagcga tgtgctcaat
     8641 ccgccgcgct aacggaggct gtgaaaaacc atgactgtat tggaaagtac gctgtcggtc
     8701 gagaaccgct atcgccagct ttttcgccag cgtctggtaa tactggcggt tatttttatc
     8761 gctatcattg cttcgctgct gctcgatttc accctcggcc cctcagggct ccctttacat
     8821 tcgctgataa agactttgat gcatccgtcc ggggcgacta acggcatgcg ggttatcgtt
     8881 tgggacatcc gtttgcccta tgcgctgatg gcgctcctgg taggaatggc gctgggtttg
     8941 gccggggcag agatgcaaac tattctgaat aactccctcg ccagcccgtt tacgcttggc
     9001 gtttcgtcgg cggcggcctt tggcgcagcg ctggcgattg ttctgggcat cggtattccc
     9061 ggcgttcccg aaagcgggtt tataccggca aacgcttttc tttttgcgct cctgtccgcc
     9121 ctgctgctcg atagtctgac ccgctggacg cgcgtgccga cgtcgggcgt tgtgctgttt
     9181 gggattgcgc tggtgtttac cttcaatgcg ctggtgtcca ttatgcagtt tgtcgccgat
     9241 gaagatactt tgcagggcct ggtgttctgg acgatgggca gcctggctcg cgcatcatgg
     9301 gagaaactgg cggtgctggc ggcggcgatg gcgattgttt taccctggtc gctgcgtcgt
     9361 gcctggcagc ttaccgcgct acgcctcggc gaagagcggg cgatgagttt tggcattgat
     9421 gttcgccgtt tgcgcctggg ttccctgctg cgcatcagcc tgctggcggc cctttcggtt
     9481 gcgtttgtgg ggccaattgg ctttattggc ctggttgcac cgcatatttc ccgcctgttg
     9541 ctgggagaag atcatcgctt ttatttgccc ggaagcatcc ttatcggtgg gctggtgcta
     9601 tcgctggcct cggtagcgtc gaagaacatc attcccggtg tcattttacc ggtaggaatt
     9661 gttacctcgc tggtaggcgt tccgttcttt ctgagcattg taatgcgtca tcgagggagt
     9721 atgtcatgag cgggcttacg attaatgcgc tgtgcgccgg ttacggcaaa cggcggatta
     9781 ttgagcatct gtcgatttct acgctgccgc gcggcgaagt cactgtactg ctggggccta
     9841 atggctgcgg taaatcaacg ctactgcgcg ccctggccgg gctaaatcgc gccagtggcg
     9901 aagcctggct gaatgaagag aatctcttat cgctgccgtt tgctcgtcgg gctgaaaaag
     9961 ttgtgttcct gccgcagtcc ctgccgcagg gcgtgcattt acaggtgctg gagtcggtgg
    10021 ttgtcgccca gcgcgcctcc ggcgccgggc aaaatcaggc gcaggctata gcgctgctcg
    10081 aagagctagg catcgcacat ctggcaatga actacctcga tagcctgtcc ggcggtcaga
    10141 agcagctggt ggggcttgca cagtcgctta ttcgccgccc cgaattatta ttgctggatg
    10201 aaccactgag cgcgctggat ctcaattatc agttccatgt gatggatgtt gtatcgcgcg
    10261 aaacgcgaag acgcaatatg gttacgctgg tcgtcttaca cgatatcaat atcgcactgc
    10321 ggcatgccgc ccaggtcatc atgctgaaag aggggaaact tatcgacagc ggcgacccgc
    10381 aaacggtgat ccatgcagag agccttgcgc aggtatacgg cgtacgcggg agagttgaac
    10441 gttgtgctca gggaagatcg atggtgatag tggatggtgc aatcgaaaaa tagtcggatt
    10501 aacgtggttg cttcagcctc aggtaaagat caataggaag tctgtaatgc aacatataga
    10561 ccgccttaat gtcattaaag cacttgtgct tctagaagat gagcagattg ttcgttttaa
    10621 catcgctgcg aacgataacg cctcgcagat ccacatgctc gttgacggtc tgggcgtcgg
    10681 gctgacacat gagcctgtcg ctgccggaat gatttcgcac cggacagctt gtcaccgttc
    10741 taggcaacat gacagacctg cgcattccta tttctattat ctatccgcct accaagttgc
    10801 ttaatgtcag gctaaggccc tttaatgact ggatttcaga ccgattttct ggacagccga
    10861 gcgggtaaat cgcagtacac tgacagctag acaataaagc tccggttcat gtttgaacca
    10921 tgtgtagtga atgtccgctg ttcgctcaca acggaccttc agttcagccg ctccggccgc
    10981 ttcatgccat gaatgtacgc agccagtcag aatattaagg tgcggatttc agttcacttg
    11041 tcagcgccag tgacacaaga cagtaatgac caacaatttg gtattgacac aacaagttgc
    11101 ggccccggtt cgtggaggag attaaacgac tgtagccggg gccactgaaa tgaagaattc
    11161 agagagactg aatgatatgt cgcaccttca gtgaatagtg taaaaactgg gatattctca
    11221 tccgggatta cccactcata ctcaatgcgt aactttagtg cggcccccac aggtaagagc
    11281 cggacacatt cgttgtgtat gctgcagaat cggtaaccgg aggccctgcg gtccttaact
    11341 cagtgtcttg tgttgctcat ttgccacaaa gtcctggtac gcctgctcaa tcatttcatt
    11401 cagcagtttt tttgggagga caccacgact tcgggcaata ctttcaagct gtttgtggat
    11461 ttttccggac agtttcagcg ggacggaacc atcagcacgt tcatcacgcc ttttttgttg
    11521 ctcccgggca cttttcagct catcaagaag aatcgcttta tgctgaatcc tgggggcctg
    11581 gaaggaggcc tgcgtaaact tcgctctgtt ccgtctggga ttactttcca gtaaaaactg
    11641 tacctgttgc tcattccagc cttcccagtt atcaaacgtg gcgcaggcga accagtattt
    11701 ctgctccggg gtaaggggat tcagcggggt tcccacacac cggacctgca tggcgttcca
    11761 taaccagtca cactgttcac tgtccctgct gtccacccac gaaaaggggg aggggtaatc
    11821 attgtttatg ctggcccatt cagagaatac tgagttgata atcgtcctcg tagcctctgg
    11881 tccggatttc agcaggtaac tggagcagaa ccatataatt tcacggtaga tattatccgg
    11941 atgcacggtg cgcatcggct tttctctggg accggcaatc ttcggttgcg atggaaaacc
    12001 catggcaaac cgcagatgtt ccgggggcat tcctgaacgt ttttcaggaa tgggtgtgag
    12061 tcctgcgttt ctggtctttt ccgcttgtcc ggtattgcct ccggccggag tgttgtcggg
    12121 agtatcttcc ggaggcgagg tgttttcatt agtgggggga acaacaatag aactgtccat
    12181 ctccggaatg gggtgagggg cataatcact gtggtagccc ggggaaagcg ttaagtcccg
    12241 aagggtgaca tcaggttccg ggcagtgagt gccaccggga gtgaacggga gttgttgctt
    12301 ttgggggaaa aatgtattgt gaatgcgggt atacagccgg aacagaaaaa aataagatgc
    12361 ccgtggttct ttttttaacc actttgtata ttcctctatg gcaccgagct gttgtttgtg
    12421 ccgttttaca gcatcgatat acggacagct ggtgcagata tcatccagtt cctttcccac
    12481 aatacacctc acctgctctt cccacatttg cagattttaa gaaagcaaac aaaaacctcc
    12541 gtacatgatg tacagaatgt acagaacaat ctatcaggta ctggcgtcag aagagctccg
    12601 ggccgcctgc attttccgga atgcgccggt tatgaaaaac cggaaattca gtctctgttg
    12661 taagatgtac cctctgatac tacgttttcg ttttctctgt gtatttaact cactgtattt
    12721 attgtctttt taagcctgac tggtattctc cggtcatagt gtgccggata tatatcccgt
    12781 aatcttctca tttttatata aaaatacctg cactctcttc agcgcatctg tctcctttaa
    12841 gtaaaaaatg ttttcacgta ttgctgtaaa ttcattttaa atcagtgtat taccttgata
    12901 ttgctgtctc ctggtttgat gcgtaatcgt tctgcctttc tggatgattc tggtgcttcc
    12961 gggtgcattc tcctctgcac atgcaacagt tcgtcatcgc cacgttccca aaaagcatat
    13021 aaaaacgaat cgaatttgat atttttttca atcgtttttc ataggaattt cgtagggtgt
    13081 tctccgctat aacctccata tttgctgtct ttttcttcat aaatcgtatg caatgtagcc
    13141 tgtctgtcaa gacctcttct ctgctgttga ggagacgatc tcatttttgg tcttttccgg
    13201 gagagaggct gtcagatgag cgagccggat ttactgtcag aggtacaccc cgtagcagac
    13261 ctgtgctccc atttccgcga tccggaacca acaacgccct acggttaata ctatttgaag
    13321 cgtcaggtac tgcaggaagt tcccccctgc gcggacatcg gttcctgccg acatctcaca
    13381 atacggtcag actgtcatgc agtaacaccc gcaatgcggt ctgcggtttt aagtgcaccg
    13441 ataatgcgtc atagtaagtc tgggggagtt acggaatgcc tgttaagtcg tgccgtgggt
    13501 ggagacagct tacttttcca cagcaactgc agtcattcag gatgatcagg agatattctg
    13561 tcaatgtcac gatcctgcca ttttcgtacc cgctaagata cttccgttac tgatgctgtc
    13621 cgcttttcac catttcatcc tcactgacag gcgttgcgtt ttgggtcatc accacagctt
    13681 tacgtttctc ttttaaccgg taacgctctc ctttgatatt cagcgtggtt gagtgatgca
    13741 gcagtcaatc caagagctct gttgtccggc aaccaccata tcaacttctt cccgggtgtg
    13801 gcggagaggt ggtatcctgg ctgagtttcg aagcgaaccg ttctttttga cgggcgcatt
    13861 ttacgtccgg gctgaatgta gtaacgcggc atggaacgtc ggcccgtatg gcccattgtg
    13921 tcacgaacgg tgcaatagtg atccacaccc aacgcctgaa atcagatcca gggggtaatc
    13981 tgctctcctg attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc
    14041 ctgcacaagc agggaatgag tagccgggcg attgccagag aactggggat ctcccgcaat
    14101 acggttaaac gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct
    14161 gttgcttcac tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat
    14221 ccttacaaaa tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc
    14281 ggaatgacca ttctcagggg attcattcgt tctctctcgg ttcctcagga gcaggagcct
    14341 gccgttcggt tcgaaactga actcggacga cagatgcagg ttgactgggg cactatgcgt
    14401 aatggccgct caccgcttca cgtgttcgtt gctgttctcg gatacagccg aatgttgtac
    14461 atcgaattca ctgacaatat gcgttatgac acgctggaaa cctgccatcg taatgcgttc
    14521 cgcttctttg gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg
    14581 caacgtgacg catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc
    14641 aaggagatgg gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag
    14701 gtggaacgga tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg
    14761 cgcccgatgg ggatcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg
    14821 cacgatgtcg ctaaccaacg aaagcatgaa acaatccagg cccgtccctg cgatcgctgg
    14881 ctcgaagagc agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat
    14941 cctggtgaaa atctggtgaa cttcgacaaa caccccctgc atcatccact ctccatctac
    15001 gactcattct gcagaggagt ggcgtgatga tggaactgca acatcaacga ctgatggcgc
    15061 tcgccgggca gttgcaactg gaaagcctta taagcgcagc gcctgcgctg tcacaacagg
    15121 cagtagacca ggaatggagt tatatggact tcctggagca tctgcttcat gaagaaaaac
    15181 tggcacgtca tcaacgtaaa caggcgatgt atacccgaat ggcagccttc ccggcggtga
    15241 aaacgttcga agagtatgac ttcacattcg ccaccggagc accgcagaag caactccagt
    15301 cgttacgctc actcagcttc atagaacgta atgaaaatat cgtattactg gggccatcag
    15361 gtgtggggaa aacccatctg gcaatagcga tgggctatga agccgtccgt gcaggtatca
    15421 aagttcgctt cacaacagca gcagatctgt tacttcagtt atctacggca caacgtcagg
    15481 gccgttataa aacgacgctt cagcgtggag taatggcccc ccgcctgctc atcattgatg
    15541 aaataggcta tctgccgttc gggcagtggg atcaaacgtt cgccggtgat gcagcactga
    15601 cctcagcgat gctggaccgt atcttacacc actcacatgt cgttcaaatc aaaggagaaa
    15661 gctatcgact cagacagaaa cgaaaagccg gggttatagc agaagctaat cctgagtaaa
    15721 acggtggatc aatattgggc cgttggtgga gatataagtg gatcactttt catccgtcgt
    15781 tgacacattg ccttaatctc cgcaaagata acctcgtcat tccggacatt ctctgccagg
    15841 catatgtcga tgtaatccat aaacgatttc agcttaaccc ttttgtggcc ggtctttctg
    15901 gctggcggtt caggggatgt tttctggtta tcgtgtacca caaaactggg atattgcccc
    15961 gatagtcaca tttcagattg atcgcggtct gaagatgtgg cactgaggta acgtgctggt
    16021 gtcactggta ttgctgaagt tatggttttt cggggtcagg tgagcctgtt gtgtgtatgc
    16081 tttttgttca gatcagaggg gggctgtgcg gacagcggct tttgtctccg ggcattcacg
    16141 cctgaacgat gaatgcgatt ttcagcaata tttgtctttg tgacactctc tggttgagaa
    16201 gctaatgcgc gcccctgttt ccacagagaa tgaggcttgc catcagccga aacatcagta
    16261 ttccacgcca tacagacggc gcctgaccat gtgctgatgc aggtaaaatg tttcaactac
    16321 aactgttcgg gttgctatgc aacgaaacag cgattctcac gatctttaac tgcaacgttt
    16381 ttccgcctga aagctgactg cctcctgctg aggtgggagg ctatgattca gtgcgtttat
    16441 agtctccggg gtatgcatgt accggagcat accatttctg gtggataaca agccggcaca
    16501 ttcagcctgc agtgaagatg gaggcacctg gttgtgaaga acgtttctgc ccgtggctgc
    16561 ttcgcatcat caatggtatt catacccgct cagaaatgac cgggtgctga agccctccgt
    16621 tatcgcgcag tataatgacg tatatgatct gtggacgagt gagcggcgtg cctgatgtac
    16681 gccgctatta ctgtatttta ccacagggct ggttttctgc tgccagccct gtggttatca
    16741 gacatcaact gtgagcggca tcaaccgatg tacggtatgc cataagcacg tactgatcca
    16801 tcagatttgt cagtggttca ctctgaccct gacgtgcaat gatccattgc tccagatctt
    16861 tttctgattt gtgtttcagg tttttcgtcc agtcgtcaag gctgtttccg gtcattaagt
    16921 caagccagca gagccagacc agtttgagac gtaactccgt gccgccaaac tgacggaaga
    16981 ggctgatcag tttgtttgcc agagaatgtt gcttcaggcg ctcacattca gacagcagga
    17041 tcccttccag catcggatga tgaaccagca ggtgttcgct ggtgcataaa ttcaccacca
    17101 gatcatcatc cagggacggg gagctccgtt tgtccggacc ataaatcagc cggtgcagac
    17161 tctggggaac gatggcaatc tccggttttt cgccattgaa tgacacccac ccgggcgtca
    17221 gattatggaa atagtagata ttcggatgac acacgcccca tgatacggcg tgccgggtga
    17281 agccggtcag ccatttctgg tagttttcat ggagcagctc gtaacgggtc tgatactgtt
    17341 gtttcatttt catattcctc acgggggata acaggcagca gagcgccgcc ggcaggccgg
    17401 agattgcgtc tgctttgggc gaagggttag tggttaaaaa ttacggacgg gactgtgcaa
    17461 tacgcgcctg cagccaggct tccacttcac ttttcagcca gcgggagctg cgccccattt
    17521 tgatgggggc aggaaagccc ccaactttga tgagtttgta aaaccactta tcggttaagc
    17581 cggtcagttg agtgataaac gccatgtcga ccatctggtc atccatcagc gaaactgggg
    17641 tagccatgag aatgttcctc cggatgttgt taaaccagcc ggtgtggtca tgaaaacagc
    17701 gcgccacctt caccggcata acaaccgggg agaaacgtaa aaaataatcg ggaatctggc
    17761 tgtccggaga taaacggggg gattgcgatg gcagagccgt agcggaatcc cggggggtgc
    17821 ccctattaag ttgtcaagca tgttatgacg cctgcggggt ataaaaagtc ccgtcgcgca
    17881 tcatggcgaa cagaacgtcg cagcgtcgtc tcgccagggc gataagcgcc tgattgtgtc
    17941 gttttccctg actcattttg cgggtgtagt aagccctgga gagcggatcc ctgagcgcgg
    18001 cgaaggccga caggaacaac gcccgtttga gagctttatt accccgtcgc gagggatgct
    18061 caccgcgtat tgacgagccg gatcgccgag ttaccggcgc aaggccagca taagcagcga
    18121 gatgtgcgac agaggcgaag gcgcggcagg cgacctcggt gaggagtctg gctgcggtcc
    18181 tgacaccgac tccgggcata ctggtcagga ccgggtaaag agggtgagca agaactcgct
    18241 gttctaccgc aagcgccacc tcgtctcttt gcttacgcag cgtgatgagc tggagtgcca
    18301 gacgtggcag tactacggca gcggcattcg tgccgggaac gacgacggtt tgttcggcca
    18361 gtgcctgagc tatgtctgct gcaaggcgtt tacccagacg aggcgcaagt ttgcagagct
    18421 gggctgccag cttcttctca cccagcgaag cgagtttttc tggtgaggga tatcgctgga
    18481 gaagatcgag taccgccggg tgatcaagtc tcggaccgag aacgcgctcc agtgccggat
    18541 gtatctgggt cagaaggccg cggatacggt tgctggcctg cgttgtctgt gcggcaagat
    18601 catcatcgaa gccgcagagc atggagagtt cggcgatttg ctcgtcagcc agtttcagcg
    18661 tgcgtagcgc gtgaggcagg gtacgggcag cttcggcaat gatggcagcg tcacgagcat
    18721 cagttttagc ttcaccggcg tgtaagtcgg ctatgcggcg catggccagt ccagggaggt
    18781 atccgacaag gactccttct gagcgggcaa cggcgacagg taacgcaccg atggtagctg
    18841 gctgatcaac aaccagcagt atctgaccat gttgtttcag gtcagatatt agcgacctga
    18901 gtttgttttc gtcgttgggt aatgctttat cgaacaggcg tttacctgaa cgattaatgg
    18961 ctacagcgtg atgcgtatct ttaccgacat caacgccgat aaagacctgg acggattcgt
    19021 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaagcagat aaccacgggg
    19081 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
    19141 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
    19201 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
    19261 agtaacgggg gtgcgaaaag atgcttccgc acgcacgcgc gataaagagg ggatagtttt
    19321 cgtgaggaca gggagagaag caggcgttct gataattgca gagttgtttt cggaggtggt
    19381 tacagtgagt cagcccgttc ctggcgagca ggagactgat atcaccgatg ggataaggtt
    19441 gtctgattgg tattataaat ataagaagtc atcagagagt aacgtaaagg atttgccaga
    19501 ataacctaag agagtaaatc tctccggtaa cagagcgctg tgaagcattc tgtatcagag
    19561 tgcaaataca tgaacgctga tgcgtaaaat cagcagtgac acgccagctc cccctatgcc
    19621 cattcctgta taagacatga actgagcgta ttaatgctcc tgccctgaga ctgagccggt
    19681 gagtgagggg ctgagaaatc cctcttgaca ctgctctaac cagaaatact tccccggttg
    19741 ttgcggtgcg tccggatccc cgataacgga acactaaggc tgtattgcgg gacaccaggt
    19801 aagtggcagc ccgtaatacg tcaggagacg cgggaaaagt gaattcccac acgcgttata
    19861 aacttaccct tgcggagctg gaagccttta actctgccgt tgacaaccgg ctggcagaac
    19921 tgaccgcaaa ttttacgagt gcgtgctggc ttcagtcttg tcacctgagc gttcggtaac
    19981 ggaggcgcgg gcggtaatag actgattttc aggtattacc ggcttacggc gtgtggttga
    20041 tattgagggg ctgtgagttt cgcgtatttg tcagaaaccc agacgagttt tcctgtatgg
    20101 gacgaaataa ggcagcggca atacctggaa aaatttaata tggtatgaag gagaacctgt
    20161 cggttacgct taatataccc ggtacactgt cagctcaggg gatacatgaa ccgttatctc
    20221 accagctaca cagtatatac acactgacgc tgacagtagt cagcaggacc acaataatca
    20281 gtgccagcag tatggtaacg atacggtctg tattccggta atagccgaag gggtcgacag
    20341 aaccacaata cgggcaacac actgtattat gagtaatcgc gtttccacag tccctgcact
    20401 tctttttgcg tatcaccatc actccctcct cacagcctta cccgtaaccg gttttttaca
    20461 ttaaaaaaac aacgctgaaa attcaaccgt cagtccggag acgaccgttc gggttatcac
    20521 agagtgcctg agacagtgcc ctgccggagg tcacttcagg gactctgcgc attttttacg
    20581 acgtggctat tccgtatgaa ccatacggag atttaaccat gacctacaaa tacaacccct
    20641 tctggcagca acgtattcgt gagacagtgc ggcacgcact gaatgttcat ccccgcctga
    20701 cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac gcagctgtga
    20761 tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa cgtaagcatc
    20821 gggaaggtaa acgcgtgcat cccaccactc tgcattacgt ctgggcccgg gagtttgggg
    20881 agtgcaaagg taaaaaacac tatcatctga tgctgctggt caaccgggat acctggtgtc
    20941 gtgccggtga ttaccgcgct ccgggatcac tggccggaat gattgaacag gcctggtgca
    21001 gtgccctggg ggtggatgcc ggatgccatg ccacgctggt gcattttccg gcctggccgg
    21061 cggtgtggct ggcgcgtaat gatgacaccg gctttcagca agtgctggaa cgtgctgact
    21121 atctggcgaa ggagcatacc aaagctcact gcaccggtga gcgcaacttt ggctgcagtc
    21181 gtggctgagc cagacagacg agttatctga cttcagtgtg atgacgtgtt catggccacc
    21241 acactgattt tcccttatga gactgaactg aaaccgccat tcacacctga ccacgctgcc
    21301 cgtaccggga cggctgttgc ctgcgtgtcg tctgacggta aggaacatat actatgtacg
    21361 caaaatcctt tctcgctctt gatagcaacg gacgtctgac gggcgcccgt actgtacagg
    21421 ccgcacctta tgctcactac acctgccact tgtgtggcag tgcactcaga taccatccgc
    21481 aatacgacac tgaacttccc tggtttgaac acactgacga caggctgaca gagcacggtc
    21541 aacagtgccc ttatgtcagg ccggagcgca gagaaataca gttgattaaa cgtctgcagc
    21601 aattcgtacc ggatgcctta cccgtggtgc gtaaagccag ctggcactgc agacaatgtc
    21661 accacgatta ttatggggag cggtactgca cacactgcca gaccggagga ttcagtattc
    21721 cccggacaac tcaggaggaa atatgcgaat tctgaactgt tatatggcga atgacagcaa
    21781 aggccatttt gttacggcga aagaagcagc gaagcacaac cgccaggacg ttttgtgctg
    21841 tgtgtcctgt ggatgcccgt taacacttca gcggggcaat gacggacaac caccgtggtt
    21901 tgaacatgac cagatgactg tcgctgagaa aatcctgctg cgatgcacct ggcttgaccc
    21961 ggcagagaaa gaggcccgtc gtttgcatct gcagggtatg acggttccgg attatacggt
    22021 gaaggtgaga aagtggtttt gtgtgatgtg tgacgaagat tatgaaggag aaaagtgctg
    22081 cccgagttgt ggtaccgggg tatacagcag ggaggggggg ctgcaggaag gcaactggaa
    22141 ggataggaac tgagcggttc agtcgcggaa atcagttgta gagctgtaat gatatctccg
    22201 ggagtgctgc gttggtaggg gcatcccggg atagagaaat tttagcactt tgccctgaat
    22261 gattgtagac gtcccgggta ctcgtaccgg tctgagttat tacagttaga tattcacttt
    22321 ttctcccgca tacttcacat ctgtcaacga cggatgaaaa gtggttcttc cgcaacctgg
    22381 gtggaagaaa atggatgaaa ctttctggcg aacaaggatc taagctgttt taagttatgg
    22441 gcaacgcaat gcactctctt aagacacttc tacagttacc ttgcggatgg cgatgcagtc
    22501 gacaaattat tagctctgac ggtataaccc tccatctcca cggaaaacgc aaaacagcac
    22561 aatgtcctga atgctttaag cgtagcgact ctgttcatag ttgtcgtcgg cgccggatac
    22621 agcatctacc ctgctcaggg cagacgctat ggcttgtatt ttccgtccgc cactggtact
    22681 gccgtaaccc ggcttgttaa cgtaaaattt ttgccgagtc gcttgctccc ttcgccggtt
    22741 cacaccagca gtcttcacag gcgttacaaa atttacaacg tcaactggga ttaatagctg
    22801 gaggtgaggc tggaaaacgg gctgcaacgg cagcgggtct ccgttgcagt gctgatactc
    22861 ttcttcgcag ggttatcaat accccggaga cgaaacagtc aggcgcgcct catgtcggta
    22921 ttgatgagtg ggcgtggcat cggggccact gctgcggtat gttaatagtc aatcttgata
    22981 ctcaccgtcc cctcgtcctg cttcccggcc gtgatcagcg tacgctggcg acctggttca
    23041 gaaaatatcc ggaaatacag gttgtctcgc gtgatcgcag tggagtctat gcgacagcag
    23101 cacgtgaagg tgcacctcag gccagacagg tggccgatcg atggcacctg ctaaaaagta
    23161 ttggtgatga gcctgaacga atgatgtaca gacatatgcc tctgatacgt cttgttgtca
    23221 gagagttatc actgaagaaa tcacctgagc cagaaatatc tgtgcctgta gcatcgctcc
    23281 gtcgtccgga acgccttaaa cagcaaaccc gcaaaaaacg gcatcagcat tggacagagg
    23341 ttatggccct gcataacaag ggatgtagtt tcagggaaat atcccgtatt acaggcctgt
    23401 cgcgagtgac agtcagtcgc tgggtgcgtt caggaacatt ccctgaaatg tcaacccggc
    23461 ctccaaagcg agggcttctg gacccatgga gggagtggtt aaaagagcaa cgagaaagtg
    23521 gtaattataa tgccagccgg atatggcggg aaatggtggc ccaggggggg acaggcagtg
    23581 aaaccatcgt cagggatact gttgccaaat ggcgtaaagg ctggaaccca ccggttacta
    23641 ctgccgcaag acttccttca gtgtcccggg taagccgctg gttgatgccc tggagaataa
    23701 tcaggggcga agaaaattat gcatcccgat ttattagtct gatgtgtgaa aaagaaccgg
    23761 agttgaaaat agcacagcaa ctggtactcg agttctaccg tattctgaaa acctaaaata
    23821 aatcacagtt aagcagctgg ttcactcgag ttcacgaaag cggctcagca gaacttcggt
    23881 gcgtagctgc ggggatggaa gctgatgctg cggctatatg tgaggcaatc agcagtcgct
    23941 ggagtaatgg tgttgtcgaa ggtcatgtaa atcgcctgaa ggtgctgata cgccagatgt
    24001 atggtcgagc cggatttgaa ctacttaggc ggagggtaat gagtccactg gcatgaaaat
    24061 cttcacctag gttgcggaag agccactttt gcactgttcg tgacagtatg gcctttatgt
    24121 actcactttg ttaccggtag ctgaatcatc actaccgtgc tgcaaatggt ggtgaacagc
    24181 atcaattgtg gaatgaatgt tgccgaagcg accaatgcgc ctcgtttcca ccatcagtgg
    24241 ttgccggact agctgcgtgt tgaaaagggt ttagtccgga tacgctcaag ctgctggaag
    24301 caaaaggtca gaaagtggcg ctgaatgagg cgatgggcag cacgcaaagc attatggttg
    24361 ggtcggatgg tgagttgtac ggcgcatccg actcgcgctt ggtggatgat ttaacggcgg
    24421 ggtattaatt gacatccccc ggcgcagtgt gccgggggat aatggtttta tgcggtcaat
    24481 aatcgtccgg gattatcgat acagagtttt ttgaggatcg ttttatcgac gccgcgctcc
    24541 gccagatagg ccagaaaaac atctggaaca aatccccagc cattaccgcc atttttagcc
    24601 cacatttgtt taaggaagac atcgtggctg agaacaagct gatcggcgta tcccaactca
    24661 atgagatgag cgacggcatc ggcagtctcc tgcacccctg gcgctattcc ctctttcggg
    24721 aaggtaatgt ctaggccaat catgtcgaat tccagccaga cacccttatc aagcattttc
    24781 cgctgatacg ccacgtcttt tcctgacgga tccgagtgtg cgagagagac tttatttggc
    24841 gagacgccca tttcacctaa cacaatgtcg agtacttcat caccgcgacg aagccagccc
    24901 ggcatgtgaa tattcatcgc cacatgagga ttgttaatct gtgccagcga ggcagcccgc
    24961 aagctgttat gctcggcttc ggtaaatgtc ggtgagacac ctatttcacc gatcattccg
    25021 gcacgaatat ccgtatcgcc aatcccttga ttcaattctt tatcgatcgt tgccgccagt
    25081 tcatcaaccg ttttatgaat tctctggctt tcaaattttt ccaggtaggg accggaggaa
    25141 gcaacaatat tcagacctgt ttttaatgcg acttctcgta atgcctgcgc atctcgtccg
    25201 atagactcag accctgtggc atcaacaatg gtgcgaccac cgagcgagat aaagttatta
    25261 atttcgaaga tcacatcttc aataggtttg cggtccatat tatcagcgca gcaataagga
    25321 tcgtgtttta atgcccactg gatttctgcc gtgacttttt tatcaacaag ccgttgtgaa
    25381 aaaggatagc aaggagcatc cacaacagag gaaagatcgt tgaacagatg ttcatgagga
    25441 agtgttagcc ccatatcttc acgggcgacg gggcccgtca cagtttgtag ataatctttc
    25501 atgattatac ctccattttc ttcattgttt tcgaggttct gtattccgga atagtggtaa
    25561 caattgcacc gaccagtgca aacagtgttc cgatgatcgt gaccagataa acggtattgc
    25621 ccagcgatgg aattaatacg tcaatcaata ctgatcccag taactggccc gccgttgaag
    25681 caacccccag catcagtaac cccagacctc tgaccaaaat ggccattaaa ccgatagaca
    25741 ttagtccaag cgggccgcca agatacatcc accagacatc tggtaattga acagtgacgt
    25801 gccctaatgc gacacgaata gctagcgctg tgcctaaaac acaaaatcca acgatgaaat
    25861 tccaggtgat tgaaaccagc atggatcccg tagcttcagc tacttttgca tttccagcgg
    25921 gctgccatcc tgcaagcaaa ccagctaaga acggaagaat ggcaagtaag atgaaagagg
    25981 tagaatgcca ctgcggtgac acaacaaaaa tcgtagcaat aaccgcaaat aatgcaccga
    26041 tgatgcgata aggggtaaag tattttttct cgtcgacacc gataccaaag cgatcgcaaa
    26101 gtaaaccaga cagcaatagc gcggagatca gtgcggtttg aaacgttgcg accccgagtg
    26161 cgctggctga tgcgccttca gaaaatacca ccatcgctcc acagagaccg gcaaaccagt
    26221 tccagagagg aacttttcgg tttttaatca gagaaggaat tgccgcaaat tgttgccggg
    26281 tttcttttcg cgccataata ataaaaaaca tcacgactaa accactggca aaggagataa
    26341 ctgcgcatgc attaccatcc tgtaaccagt gacctaattg accattcacc gctgattgca
    26401 taggggagag catacccgca agaatcgtgg caagcatcca taagggagtt gagtacttat
    26461 tctttttcat acatcaatcc ttctattata gtaaatgggg taactggagt taccccatta
    26521 ttattatctt gcaatatgag tcagtatatt tacccacaac gcggtataga acggccagga
    26581 cataaactgc tgtgtacccc agtgcggtga acagtcactc ataaagcagg ctattttccc
    26641 gttgtgatag ttaccaaaga ccagtaatgg atcgttgttt attgttaata ccacctcagc
    26701 attttcttta gcgatcgccc ggttataacc taggaaaaaa ggatattcag agaacccttt
    26761 aataactgga tgttcaggtt gtgatggctg tgcaataaca ccctctggtt tttcgacacg
    26821 atcgtcacca tctaacatcg taaccgggag cacatcagcc agaactgtat ttttataatt
    26881 ggcttttgct tcaattccca tgaatgagag atagccgcca atcatcaata acccaccacc
    26941 gttattcaca tattctttaa tcagttcgag agcgtttggt tttatgcgaa gttgataaaa
    27001 agtatcattt tgtagcagga aagtattact accaatatca ctaataacga tggcatcata
    27061 ttgttctaac tgtgccacat cttcagggaa ggcaatttgt accgtatgtg ccggcatata
    27121 ggtgacatct acctgactat ttttcagaca ttggagtaac caggtcgcac cttcttcata
    27181 tttacttgag gtaaaactgt catatccttt tgaatgaatc atatgaatat gccaggattc
    27241 cccaataaag agcactttta gtgatttttt ggtgttattc attctctgtc ccttcttaat
    27301 gcgtaaaaat agtttgctga taatgtatac tctgaattcg atgtatcaca gattcatgtt
    27361 caggcatgtc ggaagcattg cttgtctcca ccgctaggga tgcaaaggcg ctggcataac
    27421 acagtgctga ttccagtgat tttcctttcg caagtcctga tgccagagcg ccgttaaaag
    27481 catccccggc tcctgccgta tttttaacga ccgctggaaa cgctggcgaa tgaatgaatt
    27541 ttttgccatc aaatgcgagc gagcccttgc taccgagcgt gataaccgtg ttttttaccc
    27601 ctttattgtg gatagcttct gcagctcgtt tagctgattc aagatcgtgt acgtctatgc
    27661 cggacagtaa accagcttct gtttcattgg gggtgatata atcaacatcc tgaataagtt
    27721 cgttgacgat atcattatag ggtgccgggt taataatgac cgggatactg tttttttgcg
    27781 ccaatgtgat cgcttgttgc agagcggtat aatttgtttc gagctgcaga agtatgacat
    27841 cagaattaat aatcgcttct ttctgaatag ttatttcatc ggacgacatt gtcatgttgg
    27901 caccgggata gatcgcaata acattatcgc cagtaccttc attaacaagg atcgtggcag
    27961 ttccggtttg cgtttctttc gtttgataaa ttatactttt ataaatctta gacgaattca
    28021 taaagttgat cgcataatca ctaaagtgat ctgtacctac ttttgttatg aaataaacat
    28081 cagtatcggc aaagcttgct gccagcgctt ggttacagcc ttttcccccc ggtgaaaata
    28141 taaaattact tgccagaagc gattctccga tagtgggaag cctcggcaaa taactgatga
    28201 tatcaacatt aaacgaacca ataacgcata cttgactttt catagttgag tggccttttt
    28261 ttattccctg ggctgtatca taattttcaa gcgcataagc gatttcattt ttcgcaatag
    28321 tatcaagcga gtctaggacg ataaaagcac cgccatgaca tcgagtcaga tagccaagcc
    28381 tggcaagttc attaagatct gatcgaatag tttctttcgt tacattgagg ctattggaca
    28441 gatcggatac ttttacagaa ccgaatttac tgagtcgttt caatatttca tgatggcgtt
    28501 caaatttcat gcttcacctc acttcagtta cgttaatatc tatcgatgta tattgatgtg
    28561 ataatcacca taaagtggcg gggaaaagca aaaaaaagca aaaatagcct gagaaaaggt
    28621 aagcgttaat ggagaatcat attgatgctt atttattggt gagaactacg ttccatggca
    28681 ggagttcatc aatacggttg gaaggttatt ccggcagtac gctcaggata tggcgcagat
    28741 acgcttccgg atcaatgtcg ttcagaaggc aagtgccgat aagcccatac aacagtgcac
    28801 tgcgctcgcc gccgaagaac ataaagtttt tatttccgag acagatggca caaagggctt
    28861 tttccgcggc attatgtctg cctccgcctg accgtcaaca ctgtaataac agagtgcgcc
    28921 ccactgattc agtacacagc tgaaccattc acccagtctg gatttttgga tagcgtggca
    28981 tttttctcca ccatgcatgc agtgagtcca gtaacagttt gctcttcttc tgcctgacca
    29041 caagatgtgc tggactggta aacgatgggg atacctgctt acgcgagagc aataagtggc
    29101 tggccacaat tctcggtaca aaactctgct gaatagcagc gattttccca gcgtggtgcg
    29161 tcgtttgcat tttactggtt ctaagttaac aattatctgt tcactaatga ctactctaaa
    29221 aacttgtatc aggactatca cttatttaag tgatattggt tgtctggaga ttcagggggc
    29281 ctgtctagag agatataaag ataccttcac cccggtgatg ataaaataaa agtatctcag
    29341 ctattaaact gatgtacgat tcg
//
LOCUS       DAPQZD010000049        29201 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPQZD010000049 DAPQZD010000000
VERSION     DAPQZD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.049"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            44..1042
                     /gene="yiaK"
                     /locus_tag="RO454_002083"
     CDS             44..1042
                     /gene="yiaK"
                     /locus_tag="RO454_002083"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDY0933914.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMCGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1054..1518
                     /locus_tag="RO454_002084"
     CDS             1054..1518
                     /locus_tag="RO454_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDY0933915.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            1541..2461
                     /locus_tag="RO454_002085"
     CDS             1541..2461
                     /locus_tag="RO454_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HDY0933916.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            2575..3042
                     /gene="yiaM"
                     /locus_tag="RO454_002086"
     CDS             2575..3042
                     /gene="yiaM"
                     /locus_tag="RO454_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDY0933917.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            3045..4322
                     /gene="yiaN"
                     /locus_tag="RO454_002087"
     CDS             3045..4322
                     /gene="yiaN"
                     /locus_tag="RO454_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDY0933918.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            4335..5321
                     /gene="yiaO"
                     /locus_tag="RO454_002088"
     CDS             4335..5321
                     /gene="yiaO"
                     /locus_tag="RO454_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDY0933919.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            5325..6821
                     /gene="lyxK"
                     /locus_tag="RO454_002089"
     CDS             5325..6821
                     /gene="lyxK"
                     /locus_tag="RO454_002089"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDY0933920.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            6818..7480
                     /gene="ulaD"
                     /locus_tag="RO454_002090"
     CDS             6818..7480
                     /gene="ulaD"
                     /locus_tag="RO454_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDY0933921.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            7473..8333
                     /locus_tag="RO454_002091"
     CDS             7473..8333
                     /locus_tag="RO454_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDY0933922.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMLETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            8327..9022
                     /gene="araD"
                     /locus_tag="RO454_002092"
     CDS             8327..9022
                     /gene="araD"
                     /locus_tag="RO454_002092"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDY0933923.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(9054..9869)
                     /locus_tag="RO454_002093"
     CDS             complement(9054..9869)
                     /locus_tag="RO454_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0933924.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            10095..11495
                     /locus_tag="RO454_002094"
     CDS             10095..11495
                     /locus_tag="RO454_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933925.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLVVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            11506..13479
                     /locus_tag="RO454_002095"
     CDS             11506..13479
                     /locus_tag="RO454_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDY0933926.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQVDN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(13656..15194)
                     /gene="aldB"
                     /locus_tag="RO454_002096"
     CDS             complement(13656..15194)
                     /gene="aldB"
                     /locus_tag="RO454_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDY0933927.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSYGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(15302..16597)
                     /locus_tag="RO454_002097"
     CDS             complement(15302..16597)
                     /locus_tag="RO454_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDY0933928.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(16727..17878)
                     /gene="yiaY"
                     /locus_tag="RO454_002098"
     CDS             complement(16727..17878)
                     /gene="yiaY"
                     /locus_tag="RO454_002098"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDY0933929.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(18069..19913)
                     /gene="selB"
                     /locus_tag="RO454_002099"
     CDS             complement(18069..19913)
                     /gene="selB"
                     /locus_tag="RO454_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDY0933930.1"
                     /translation="MIITTAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(19910..21301)
                     /gene="selA"
                     /locus_tag="RO454_002100"
     CDS             complement(19910..21301)
                     /gene="selA"
                     /locus_tag="RO454_002100"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDY0933931.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(21399..22007)
                     /locus_tag="RO454_002101"
     CDS             complement(21399..22007)
                     /locus_tag="RO454_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDY0933932.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(22078..23214)
                     /locus_tag="RO454_002102"
     CDS             complement(22078..23214)
                     /locus_tag="RO454_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDY0933933.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLEPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(23217..23579)
                     /locus_tag="RO454_002103"
     CDS             complement(23217..23579)
                     /locus_tag="RO454_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDY0933934.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            24116..26029
                     /gene="mtlA"
                     /locus_tag="RO454_002104"
     CDS             24116..26029
                     /gene="mtlA"
                     /locus_tag="RO454_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDY0933935.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            26349..27497
                     /gene="mtlD"
                     /locus_tag="RO454_002105"
     CDS             26349..27497
                     /gene="mtlD"
                     /locus_tag="RO454_002105"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDY0933936.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            27497..28084
                     /gene="mtlR"
                     /locus_tag="RO454_002106"
     CDS             27497..28084
                     /gene="mtlR"
                     /locus_tag="RO454_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDY0933937.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(28095..28304)
                     /locus_tag="RO454_002107"
     CDS             complement(28095..28304)
                     /locus_tag="RO454_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0933938.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            28588..28950
                     /locus_tag="RO454_002108"
     CDS             28588..28950
                     /locus_tag="RO454_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDY0933939.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         6965 a         7754 c         7481 g         7001 t
ORIGIN      
        1 attttaagcc aaaaaagcga tcaaaaaaac aaggaagcct gggatgaaag tgacatttga
       61 gcagttaaaa gcagccttta atcgggtctt aatttcacgc ggcgttgaca gcgaaacggc
      121 tgacgcctgt gcagagatgt tcgcccgcac caccgaatcc ggcgtttatt ctcacggcgt
      181 taatcgtttc cctcgtttca ttcaacaact ggaaaacggc gatatcattc ctgatgccca
      241 acccaaacgt ataaccagcc tcggcgcaat tgaacagtgg gacgcccagc gttcgatcgg
      301 taacctgaca gcgaaaaaga tgatggatcg cgccattgaa ctggctgccg atcacggtat
      361 tggtctggtg gcactacgta atgccaacca ctggatgtgc ggcggcagct acggctggca
      421 ggcggcggaa aaaggctaca ttggcatttg ctggaccaac tccatcgccg taatgccacc
      481 gtggggcgca aaagagtgtc gcatcggcac caacccgctg atcgtcgcca ttccttccac
      541 gccgatcacc atggtcgata tgtcgatgtc gatgttctct tacggcatgt tagaagttaa
      601 tcgcctggct ggtcgtcagc tcccggtaga tggtggcttt gatgatgagg gcaatttgac
      661 caaagaacct ggcgttatcg agaaaaatcg ccgcattttg ccgatgggct actggaaagg
      721 ttcgggcatg tcgattgtgc tggatatgat cgctaccctc ctttctgacg gcgcatcggt
      781 tgccgaagtc accgaggaca acagcgacga atacggcatt tcacaaattt ttattgccat
      841 tgaagtggac aagcttatcg acggtcccac ccgcgatgcc aaactgcaac gcattatgga
      901 ttacgttacc agtgccgagc gtgctgacga aaatcaggcc attcgcttac ccggccacga
      961 atttactacc ctgctggcag aaaaccgccg taacggtatt accgttgatg acagcgtgtg
     1021 ggcaaaaatc caggcgttat aaggagataa gccatgattt ttggacatat cgcgcagcct
     1081 aatccgtgcc gtttgcccgc cgccattgaa aaggcgctcg attttctgcg caccaccgac
     1141 ttcaacgccc tggatccggg cgttgtcgaa atcgacggca agaatattta tgcgcaaatt
     1201 atcgatttaa ccacacgcga agcggtggaa aaccgtccgg aagtgcatcg tcggtatatc
     1261 gatattcagt ttctggcgtg gggtgaagag aaaattggca ttgccattga tacgggaaac
     1321 aataaagtca gcgaatcact attagagcag cgcgatatta ttttttatca cgatagtgaa
     1381 catgaatcat ttatagaaat gataccgggt agttacgcta ttttcttccc gcaggatgta
     1441 catcgcccgg catgtaataa aaatgccact acggcaatac gcaagatcgt ggttaaagtg
     1501 gccctcgctg ctttataaaa aatgattact ggagcacata atgaaaacga ataatgccgg
     1561 ttatattatc ggcgcgtatc cctgtgcacc ttcttttcac caaagaagtg aagaggaaga
     1621 gaaggatttc tggcgacaat tatctgagac tccagacatt ttgggtctgg aacaaccttg
     1681 cctggaaaat tttcaccctc ttggcgatca atggttactc cgtcatacgc cagagagctg
     1741 gaaatttgtc gtcaccgcag tgatggaaac catgcgtcgc cgcagcgaaa acagcgggtt
     1801 tggtctcgcc tccagtgatg aagaacagcg ccaggcctgc gtggcgtact atcgccacct
     1861 gttcaataaa atcaacgcat tgcaggctaa taaagtgctc gcactggcgc tccaggctgc
     1921 accgttagca acgaatccta atgttatgca agcgacggac gcttttgccc gctcattgaa
     1981 agaaatcgcc agttgggact ggccgtgcaa gctggtgctg gaacactgcg acgccatgac
     2041 atcttcttcc ccccgaaaag gctttttgcc tttagaaaat gtgctggaag ttattaccga
     2101 ttatgacatc agcatctgta tcaactgggc gcgctctgcc attgaagggc gcaatacaac
     2161 gctgccgctg acccacaccc aaatggcgaa acaagccgga aaactcggcg cgttgatgtt
     2221 ctctggtacg acgctaaacg gggcatacgg cgaatggcag gatttacacg caccgtttgc
     2281 accattctgc gcagaaagcc tgatgaccac agatcatgtt cgcgagttat ttaatgtagc
     2341 agaatcgtca acattgcatt ttgctggcat caagctactg gaaataaatg cgacggctga
     2401 tgtgcaccat cgcatcgaaa tattacgtaa cggcattcac tctctgaatg aatctcgtta
     2461 aatagatccg cttatatatc actttgaata tctaattagc cgttttctga taattattac
     2521 gaatattaac aacattagcg cataaaaaat attgcataac cagaaggact cgttatgaaa
     2581 cggatactcg aaggcattct tgcgatcatc atcgcaatac tgtcttgtat tatttttatt
     2641 aatatcattc ttcgatatgg attccacaca agtatattat cgattgatga attatcccga
     2701 cttttatttg tctggttaac ctttattggc gcaatcgttg cttatatgga caacagccat
     2761 gtgcaggtta cttttttagt cgaaaaactc tcaccggcta atcaacaacg tgtttcgctg
     2821 ctaacccata cgctcatttt attgctgtgt ctggggctgg cctggggcgc tatagaaaaa
     2881 acagcgcaag actggagcaa cttatcgccc attcttggcg tccccgttgg cttaatgtac
     2941 gccgccgcta tccccaccag tctgattatc gcattgctgg aattgcgtca cttataccgg
     3001 cagttcacaa atacccccag ccgaaaccaa caaggagcct gacgatgaca atccttatat
     3061 ttattgtcag tttgttaggg gctatcgcaa ttggtgttcc catcgcctgg gcattattgt
     3121 tgtgcggcat ctcattgatg ttctggatgg atatctttga tgtgcaaatt ctggcacaaa
     3181 cgttggtaaa tggcgcagac agcttctccc tgttagccat ccccttcttt gtactggcgg
     3241 gtgagatcat gaacgcgggc ggcctgtcgc aacgtatcgt cgacttgcca atgaaactgg
     3301 taggccacag gccaggcggt ctgggttacg tgggcgtgct ggcagcgatg atcatggcaa
     3361 gcctgtccgg ttctgcggtt gctgataccg cagctgtcgc ggcgctgctg gtgccgatga
     3421 tgcgccaggc caactatccg gtaaaccgcg ccgccgggct tatcggttcc ggcgggatta
     3481 ttgcggccat tatcccacct tccataccac tcattatctt cggcgtctcc agcggactct
     3541 ccatcagtaa gctgtttatg gcgggcatcg caccgggcat catgatgggc gtcacgctga
     3601 tggttacctg gtggtggcag gcaaaacgac tgaatttacc ttgtcagccg aaagcctcac
     3661 tgcgagaagt ctggcaatcc ttagtttccg gtatttgggc gctcttttta ccgataatta
     3721 ttattggcgg tttccgttcg gggttattta caccgaccga agcaggcgcg gttgccgcct
     3781 tctacgctct gttcgtctcg gtggtggtct atcgcgagat gaccttctcc acgctttatc
     3841 atgtgctgat taacgcggca aaaaccacat ccgtggtgat gttcctggtg gcctctgctg
     3901 cggtatctgc atggcttatt acgatcgccg aattacccat gatggtatcg gagctattac
     3961 aaccgttggt tgattcgcct cgcttattat tcatcgtcat tatgctcgct attatggtga
     4021 tcagtacggt catggactta acgcctaccg tattaattct tacaccagta ttaatgccat
     4081 tagtgaaaga agcgggaata gatccggtct atttcggcat catgtttatt atcaactgtt
     4141 caatatcact gattacgcct ccagtcggta atgttcttaa cgtggtatgc ggcgtggcga
     4201 aacttaaatt tgacgatgcg gtaaaaggcg ttgcgcctta cgtcatggtt ttattcatgt
     4261 tgttagcatt atttattttt ataccggagc tcattaccgc tcctcttaaa tggatgagtt
     4321 aaaaggaaat cataatgaaa ttacgttctg taacctacgc tttattaatt gccggagtgg
     4381 cgacgttcag cacatcttct ctggcggcac aatcattacg tttcggttat gaaacctcac
     4441 aaaccgattc acaacatatt gcggcgaaaa aatttaatga attactgcag gaaaaaacca
     4501 aaggcgaact gaaattaaaa ctgttcccgg atagcaccct tggtaatgcg caggcgatga
     4561 tcagcggcgt gcgtggcggc actattgata tggagatgtc tggctctaac aactttacgg
     4621 gactctcgcc cgtcatcaac ctgctcgatg tcccgttcct gttccgcgat accgctcacg
     4681 cgcataaaac gctcgacggc aaagtcggtg atgacctgaa agtctccctt gaaggtaaag
     4741 ggctgaaagt gctggcttac tgggaaaacg gctggcgcga tgtcaccaac tcacgccagc
     4801 cagttaaaac acctgccgac ctgaaaggac taaaaatccg caccaacaac agcccgatga
     4861 atatcgccgc atttaaagtt tttggcgcta acccgatccc gatgccgttt gccgaagtct
     4921 ataccgggct ggaaacccgc actatcgacg ctcaggaaca cccgatcaac gtcgtctggt
     4981 cagcaaaatt tttcgaagtg cagaagtacc tttctctgac ccatcacgcc tattccccac
     5041 ttctggtggt gatcaacaaa gcgaagtttg atggcttaac cccggagttc cagcaggcac
     5101 tgatctcatc tgcgcaggaa gcgggtagct atcagcgcaa actggtcgcc gaagatcagc
     5161 aaaaaatcat cgacggtatg aaagaagcgg gcgtggaagt catcaccgat ctcgaccgca
     5221 aagcctttag cgatgcgctg ggtacccagg tccgtgacat gtttgttaaa gatgttcccc
     5281 agggtgctga tctgctgaaa gccgtggatg aggtgcaata aaccatgacg caatactggc
     5341 tggggttaga ttgtggcggt agctggctga aagccgggct gtatgaccga gaaggccggg
     5401 aggcgggcgt gcagcgcctg ccgctgtgcg cattaagtcc acagccaggc tgggcagagc
     5461 gcgacatggc agaactgtgg caatgttgca ctgctgtcat tcgctccctg cttacccatt
     5521 ctggcgtcag cggggaacaa attgtcggta tcggcatctc cgcgcaggga aagggcttat
     5581 ttctacttga taaaaacgac aaaccgctcg gtaacgccat tttgtcctcg gatcgccggg
     5641 cgatggaaat cgttcgtcgc tggcaggaag atggcatccc ggaaaaactc tatccgctga
     5701 cccgacaaac cttatggacc gggcatccgg tgtcgctgtt acgctggctg aaagagcacg
     5761 aaccagaacg ctacgcgcaa attggctgcg tgatgatgac gcacgactac ctgcgctggt
     5821 gtttaaccgg cgtcaaaggt tgcgaagaga gcaatatttc tgagtccaat ctctacaaca
     5881 tgagtcgcgg ggaatatgac ccgtgcctaa ccgactggct ggggatcgct gaaatcaatc
     5941 acgccctgcc gcctgttgtc ggatctgccg aaatctgcgg ggaaatcacc gctcagacag
     6001 ccgtactgac tggtctgaaa gcgggtaccc ccgttgtcgg cggcctgttt gatgtggttt
     6061 ccaccgcact ctgcgccggg ctcgaagacg aattcaccct caatgcggtg atggggacct
     6121 gggcggtgac cagcggcata acccacggtt tacgtgacgg tgaagcgcat ccgtatgtct
     6181 atggtcgcta cgttaacgat ggtcaattta tcgttcacga agccagcccc acctcttccg
     6241 gcaatctcga atggtttacc gcacagtggg gagaaatctc atttgctgag attaatcagg
     6301 ccgtagccag tttgccgaaa gccggtggcg ctctcttttt cctgccgttc ctgtacggca
     6361 gcaacgccgg actcgaaatg accagcggtt tctacgggat gcaggccatt cacacccgtg
     6421 cacacctctt acaggctatc tatgaagggg tggtgttcag ccatatgacc catctcaacc
     6481 ggatgcgcga acgttttact gatgtgcaca ccctgcgcgt cactggcggc ccggcgcact
     6541 ccgatgtctg gatgcaaatg ctggctgacg tcagcggtct gcgtatcgag ctgccgcagg
     6601 tggaagaaac cggctgcttt ggtgcggcac ttgccgcccg cgtcggtacc agggtttatc
     6661 gcgatttcag cgaagcccaa cgtgacttac agcacccggt acgcaccctg ctgccggata
     6721 tggccgccca tcagctttac caacaaaaat accaacgcta tcaacatctc attgccgcac
     6781 ttcagggtta tcacgcccgc atcaaggagc acacattatg agtcgaccac ttctgcaact
     6841 ggccctcgac cactcatcac ttgaagccgc gcagcgcgac gtgacgctgt taaaagacag
     6901 cgtcgatatt gtcgaagcag gcaccattct ctgtttaaac gaagggcttg gcgcagtgaa
     6961 agccttgcgc gaacagtgcc cggacaaaat catcgttgcc gactggaagg tcgccgacgc
     7021 gggtgaaacg ctcgcgcaac aggcatttgg cgcaggcgct aactggatga ccatcatctg
     7081 cgccgcgccg ctcgccacgg tggaaaaagg ccacgcgatg gcacagaact gcggcggtga
     7141 aattcagatc gagctattcg gcaactggac gctggacgat gcccgtgact ggcatcgtat
     7201 tggtgtacga caggcgattt atcatcgtgg tcgtgacgcg caggccagcg ggcaacagtg
     7261 gggcgaggcc gatctggcac gcatgaaggc gctttccgat atcggccttg agctttccat
     7321 taccggcggg attacccctg ctgacctgcc gctgtttaaa gagatccgcg tgaaagcgtt
     7381 tattgccgga cggtcgctgg caggcgcagc aaatccggcg caagtggccg ctgattttca
     7441 tgcgcaaatc gactccatct ggggaggcaa gcgtgcgtaa tcatccgtta ggtatttatg
     7501 aaaaagcgct ggcgaaagat ctctcctggc cggagcggct ggtgctggca aaaagctgtg
     7561 gttttgattt tgtcgaaatg tcggtggatg aaaccgacga acggttgtca cgtcttgact
     7621 ggagcgccgc acaacgtgct tcgctggtca ctgcgatgct cgaaacgggt gttgctattc
     7681 cgtcaatgtg cctgtccgca catcgtcgtt ttccttttgg tagccgggac gacgccgtgc
     7741 gccagcgggc gcgggagatt atgagtaaag cgattcgcct ggcacgcgat ctcggcattc
     7801 gtactattca actggcaggt tacgacgtct attacgaaga tcatgacgaa ggcacccagc
     7861 aacggtttgc tgaagggctg gcgtgggcgg ttgaacaggc agcggcatca caagtcatgc
     7921 tggcggtgga gattatggac accgcgttta tgaactctat cagcaaatgg aaaaagtggg
     7981 acgaaatgct tgcgtcaccg tggttcaccg tttacccgga cgtcggcaat ctcagcgcct
     8041 ggggcaatga tgttcccgcc gaactgaaac tgggcatcga ccgtatcgcc gccatccacc
     8101 tgaaagacac tcaacccgtt accgaacata gccccggaca gttccgcgat gtgccgtttg
     8161 gcgaaggctg tgtcgatttt gttggcatct tcaaaacgct gcatgagctg aactatcgcg
     8221 gttctttcct gattgagatg tggaccgaaa aagccaaaga gccggtgctg gagattattc
     8281 aggcgcggcg ctggattgaa gcgcgtatgc aggaggccgg atttatatgt tagagcaact
     8341 gaaagctgac gtgctggcgg caaacctggc gcttcccgtt catcatctgg tgacgttcac
     8401 ctggggcaat gtcagcgcga tagacgaaac gcggcaatgg atggtgatta agccttccgg
     8461 cgttgagtat gaggtaatga ccgccgacga tatggtggtg gttgagatag ccagcggcaa
     8521 ggtggtggaa ggcagcaaaa agccctcttc cgacacgcca acgcatctgg cgctttaccg
     8581 tcgctttgcg gagattggcg gcatagtgca tacccactcg cgccacgcca ccatctggtc
     8641 gcaggccgga cttgatctcc ccgcctgggg cactactcac gccgattatt tttacggagc
     8701 catcccctgc acgcgcctga tgaccgcaga agagatcaac ggtgagtatg aataccagac
     8761 cggcgaagtg atcattaaaa ccttcgaaga gcgtgggttg aacccggcac aaatcccggc
     8821 ggtactggtg cattctcacg gcccgttcgc ctggggaaaa aatgccgccg atgcggtgca
     8881 taacgccgtg gtactggaag agtgcgccta tatgggtctg ttctcgcgcc agcttgcgcc
     8941 acagctccct gcgatgcaaa acgaactgct ggataagcac tacctgcgta agcatggggc
     9001 caatgcctat tacgggcagt aatccctcac gccggggctt catcgccccg gcactacgaa
     9061 ttgatatgtt ccttgctgta acgccgcttc cacgctgctg gcgttaagcc ggtgtgtttg
     9121 cgaaaaatct gccggaaata gccgacgtca ttaaacccgc acaacgccgc cacttccgtg
     9181 agcgatcgcg cttcgctaat taacagcttt tcggcttcgc gcacccgctg gcggtggatt
     9241 gcttctgtta acgtcagatg aaacacccgc cgatagacgc gcccaagata atcggcattg
     9301 cagtacagct cgcgggctaa aagcgccgtg gaaagcgggc ggtgatattg cgtgcgaatc
     9361 agtttctgcg ctttccaggc aagggtggtg cctgcaccat cggcctgttc atcaagacgc
     9421 gcgtcacggg tgagctgctg caaaatcagc agcacaataa actccagcgc cggagtgcgt
     9481 tgatgctttt cctgttcgct taagaactgg cgaaacaagg ccaccagcac ctgcggatcg
     9541 gcaaccttag tttgttgcgg cacgctaaga tgagtgagcc agcgcggatc gttgtccggc
     9601 gctttaacct caaaatgcag ccagtaaaat ttgagatcgg cgggaaaatc ctccacccca
     9661 acatgctgtc ggttcggcca gagtaataaa ctctctcccg catcgaccag aaatatccgt
     9721 tcatcttcct gaatcgttaa tcgacctttt tcgacgaaga taatttccca tgactccagc
     9781 cgccttgcag gatgcctccc tacgccacgg gaaataaata atccaccatt ttgcacttta
     9841 atcggtaacg tcatggataa ttcgagcatt gatatgtaat ttcccgctta ttcattaatg
     9901 attaaacaca ttaccttttg attattaatc aaaaacatta atttcacctg atccatttca
     9961 cacttttaaa ttcaggtcgg aatcgtcacc tttttttact cttttctcgc cttgttgata
    10021 ttcccgttca cggcagaatt aatcaggtca ttgcaataaa actaaaaatt gctccctatt
    10081 cacgaggaat atctatgacc tctacaccga ttaccaccgc tgatatcgct aaaggtaaaa
    10141 ttgacgatgc gttatctgta cgggaaaaaa taggctacgg cctgggtgac gcaggcggca
    10201 ccgtaataac ttgcctgatc atgaactttc tcaccttttt ctacaccgac gtttttggct
    10261 taactccggc gctggttggc acgctgttta ttgcgctacg cgtgtttgat gccatctccg
    10321 acccggtgat gggcgtcatt gccgaccgga cgcaaagtcg ctgggggcgc tttcgtccgt
    10381 ggcagttatg gattgccatt cccatcggca ttatcggcat cctgacattc accgtgccag
    10441 atgccagcat gggagtaaaa atcgcctggg cgttcggtac ttacctgctc ctttcagtcg
    10501 gttatacagc catcaacgta ccgtactgcg cgctgatcaa caccatgacc acccgccaca
    10561 atgaagtgat ctcctgccag tcctggcgat tcgttctctg cggcgtagcg ggatttctgg
    10621 tttcggtagg cttaccgtgg atggtagatc tcttcggtca gggcaacgct gcacgcggct
    10681 atcaacttgg cgtcggcgta ttgtgcgcca ttgccgtggt gatgttcctg tgctgtttct
    10741 tctgggttcg tgaacgggtg ccactctcca caatggggaa atttaccctg cgcgaacatc
    10801 ttgccgggct gcggaacaac gaccaactgc tgctgatgct ggtcatgtct ttcctgctga
    10861 ttaacgtctt taatattcgc ggcggtgggt atatgtactt cattacctac gtcttacaag
    10921 gcagtacggg ctacacgtcg ctgttcttca ccatggtcac cttcgcctcc attatcggct
    10981 cggtgattgt cagcccatta acgcggcgtt tcgataccgt caaaatttac tactacacca
    11041 acctgctcct cgctgcactg gcggtgttga tgtggttcct gccctccagc ccggcttatc
    11101 aaacgctgtg gctggtggtg atcctcggta atggcgtgat tcttggcttc acattgccac
    11161 tgcacttctc attgatggcc tttgccgatg actacggcga gtggaaaacc cgcgtacgtt
    11221 cttccggcat gaacttcgcc ttcaatctgt ttttcatcaa gctggcctgg gcctccagcg
    11281 ccgggatcat cagcctgctg tttatttttg tcgcctacca gccaggcgtg gaaaaccaga
    11341 ccgccagttc gcttggcggg atcgcggcga tggaaacatt gctgcctgcg ctgttccacc
    11401 tgctgctggc gatggcgatc cgcttttgca aactcaataa ccctatgatg tcacgcattg
    11461 ctagcgacct gcgtcagcgt catgtacagc cttaaggagc acaagatgaa catttcggaa
    11521 gtcgatctgc ataaactgac ggtcagcgat ccgttcctcg gtcagtacca acaactggtc
    11581 cgcgacgtgg tgattcctta tcagtgggat gccttgaacg atcgtatccc agaagcggaa
    11641 cccagccatg cgattgaaaa ctttcgcatt gccgccggac ttcaggaggg tgaattttac
    11701 gggatggtgt ttcaggacag cgacgtcgcc aaatggctgg aagcggtagc ctggtcgctg
    11761 tgccagaagc cggacgccga actggaaaaa accgccgacg aggtgattga actgatcgct
    11821 tccgcccagt gcgaagatgg ctatctcaat acttacttta cggtaaaagc gcccgaagaa
    11881 cgctggagca atctggcgga gtgtcatgaa ctttactgcg ccggtcatct gattgaagcc
    11941 ggagtcgcct tcttccaggc tacgggcaag cggcgcttgc tggaagtggt ttgccgtctg
    12001 accgatcata tcgacagcgt atttggtcca gatgaaagta agttacatgg ttatcctggt
    12061 cacccggaaa ttgaactggc actaatgcgc ctgtatgaag tgaccgaaga gccgcgctac
    12121 ctggcgctga cgaactattt tgtcgaacag cgtggtgcgc aaccgcacta ttacgaccag
    12181 gaatatgaaa agcgcgggca gacctcgcac tggcacacct acggcccggc gtggatggtg
    12241 aaagacaaag cctacagcca ggcacatttg cccatcgcac agcagcaaac cgccattggt
    12301 cacgcggtac gttttgtcta cctgatgacc ggcgtcgcgc atctcgcgcg tttaagtcac
    12361 gatgaaagca agcgtcagga ctgcctgcgg ctgtggaaca atatggccca gcgtcagtta
    12421 tatattaccg gcggcattgg ctcacagagc agcggtgaag cgttcagcag cgattacgat
    12481 ctgccgaatg acacggtata cgctgaaagt tgcgcctcca tcggcctgat gatgtttgcc
    12541 cgacgaatgc tggaaatgga aggcgacagt caatatgccg atgtgatgga gcgcgcactg
    12601 tacaacaccg tgctcggcgg catggcgctg gatggcaaac atttcttcta tgtgaatccg
    12661 ctggaagtac atccaaaatc gctgaaattc aaccatatct acgatcacgt taagccgatc
    12721 cgccagcgtt ggtttggctg cgcttgttgt ccgccaaata tcgcccgcgt gctaacctcg
    12781 attggtcatt atctctacac gccgcgtgaa gatgcgttgt atatcaacat ctacgcagga
    12841 aacagcatgg aggtgccggt agaaaatggc acgctgcgcc tgcgggttag cggaaactat
    12901 ccgtggcagg aacaggtgac gattgcggtt gaatcacccc agccggtgcg tcatacgctg
    12961 gctttacgtc taccggactg gtgcatacag ccgcagatca tattgaatgg ggaagaggtc
    13021 gagcaggata ttcgtaaagg gtatttgcac attacccgtg aatggcagga gggcgacacg
    13081 ctgaatctga ctttgccaat gccggtacgt cgcgtttacg gtaacccgct ggtgcgtcac
    13141 gtcgccggaa aagtggcgat tcagcgcggc ccgctggtgt attgtctgga acaggtcgac
    13201 aacggcgagt cactgcataa tctgtggctg cccgccgatg cgccatttac gacatttgaa
    13261 ggtaatggat tgttccgcca taagatctta atccaggcac cgggttaccg gtatgaacag
    13321 agcaatccag agcagcaacc gctgtggcat tacgacagcg cgccagccaa acgccagccg
    13381 caaattctga cgtttatccc gtggtttagc tgggccaacc ggggtgaagg cgaaatgcgg
    13441 atctgggtga atgaggaaac cagtgccatc cgtaggtagg ataaggcgct cgcgccgcat
    13501 ccaacgcatg gtgcacattg ccggatgcgg cgtgaacgct ttatccgacc tacaaaagca
    13561 tgcaaattca atatattgca tgagctatgt aggcatgata agacgcgaaa gcgtcgcatc
    13621 aggcaatgaa tacccgatgc gaccagcttc ttacatcaga acagccccaa cggtttatcc
    13681 gagtagctca ccagcaggca cttggtttgc tggtaatgct caagcatcat tttgtgggtt
    13741 tcacggccaa tgcctgattg tttgtagccg ccaaacgccg catgtgccgg gtaagcgtga
    13801 taacagttgg tccacacgcg ccctgcctga atgccgcgcc ccatcttata ggccagatta
    13861 ccgttgcggc tccagacgcc cgcgcccaga ccatattgcg tatcgttcgc cagctccagc
    13921 gcttcttcca tcgttttgaa ggtggttacc gccagcaccg ggccaaaaat ctcctcctgg
    13981 aacacgcgca tattgttctg accaaacaga atcgtcggtt cgaggtagta gccgtctttc
    14041 agttcgcctt ccagcagctt acgtcgcccg ccggtgagca catcagcgcc ctctttttta
    14101 ccgatatcaa tgtagttgag gatggtttcc agttgcccgt aagaaacctg cgcgcccatt
    14161 tgcgtcacgc tgtcgagcgg gttaccgctg cgaatgcttt cgacacggcg gatagcgcgt
    14221 tccataaagc gttcgtagat agactcctgc actaaagcac ggcttggaca ggtgcaaact
    14281 tcgccctggt taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa aaaggcatct
    14341 tcttcatcca tcacatcagc aaagaagata tttggcgatt tgccgcccag ctccagcgtc
    14401 accggaataa tgttttgcgt ggcgtattgc ataatttgtt ggcccacttc cgttgagccg
    14461 gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat attcgccaat ttccccacct
    14521 gcgccattga ccacgttcac cacccctggc ggcagtaaat caccgacgat ttccattagc
    14581 agcagtacag aaagcggagt aagacgtgcg ggtttcagca ccacacagtt gcccgccgcc
    14641 agcgcggggg ccattttcca gctcgccatc agcagcggga agttccacgg gataatctgc
    14701 cccaccacgc ctaacggttc atggaaatga taggccacgg tttcgctatc cacttcactg
    14761 atcccacctt cctgcgcccg aatacacgag gcgaaatagc gaaaatgatc aatcgccagc
    14821 ggcacgtcgg cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt ttctgccgtc
    14881 gctaaaagtt caaggttttg ttccattcga tcggcaatct taaacagaat cgccgcgcga
    14941 tcctgcaccg aggtgtgcgc ccatttatct ttcactttat gcgcagcatc cagcgccaga
    15001 tcgatgtctc gtttgcccga agacgccact tcgcacagta gctgcccggt caccggcgtc
    15061 aggttctggt aatactcgcc atcggcaggc gctacccatt cgccaccgat aaagttgtcg
    15121 tagcgggctt ttaatttgag ggggaaacca tactcgccgg gcttaatctg tgctgaaggg
    15181 ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta gacgtgactg
    15241 gcgaaatctt cgccagtcgg taacagcttt acgacggcta tcacgaattt acgggcaaaa
    15301 gctacagcgg gtagtgtgcg acgtacactg cttgctggtt attaattctt ttctcttgcg
    15361 cgaatccttc tttaacaatt gtccgtaaat cagctcgagc cgtattatca gagacgccca
    15421 agctcgcgct aatttcagca actgtgtact ctttccccgg attggcgagc atcacattca
    15481 ggagcgttac ctggcgcgga ttcaggcgtt taagcacacc agagctaaac agcacactat
    15541 cgagttttgc gcttcgttgt gtaatttcat cgatatgctg ctgccagttt tgcagagcac
    15601 gtttaatgac acccgcctga tattcaagga aataagtcag atccatacca tctgattcag
    15661 tgtactggta gctggcggca tatttgaccg gtgcagcatg aagcaaacgg ctgatggaaa
    15721 tatatttaaa gacatcgtag cccgatttca gcatatacca gtaaaacagc gcccgactgg
    15781 tgcgcccgtt gccatcccga aacggatgtt catgggccag cataaaatgc agaatgcagg
    15841 cacggaccaa cggatgaata tacccttcat ggctgtttaa ccactggcaa actttttcca
    15901 gccgttccgg taacaatgcc gcagcgggcg gttgatgcac tatattgcca tcatagtcgg
    15961 cgatcacaac atcatctgtt tcacgaaatt ctcccggacg atatttcgcg tcattgattc
    16021 cacccatacc aatggcatgt aactggcgaa taagcgcagg tgtcagcggt tctgcgagta
    16081 agtgcggaat ttccgccatc agccgggcat ttcccgcaat catatgctcg tcttcagtgc
    16141 gtggcgaccg ccctgtttcc agcatttttc tcgccaccag cgttgtagta ttcgcacctt
    16201 ccagctggga gctggtgatc ggctcttcca ggcgtaactg gcttaactcg gctccggccc
    16261 cgcgtaaccg ttcagtctga tctttcagcg ccagcgcggt agcacgtttg tcgacaagct
    16321 cgcacgcttc tgcaatcacc ggggtaatgt tgaatccggc ctgttcaccc gcttcattgc
    16381 aataattaat tcgttggatc gccgcgttgc gcgccagacg ggtaagcgtc cacgccacat
    16441 cgacgttttc tccttttttg atgcggtact gcaactcatc aaaaggcaga taacgtcctt
    16501 tttcatcggt aacccggtta aagcgcaggt gggccaggag ttcctcaccc gataagtgtg
    16561 ataaaggaat aagtccggga ggtgatttga tagccatgtg cttatctcaa aaaagcgcaa
    16621 tattgagata gtgtatagca ggaaagagaa actatctcaa aaaaagcaag ttttgagata
    16681 gttgaaaaag cggctgacaa ctcgccagcc gttgtgggaa tgatgattac atcgctgcgc
    16741 gataaatcgc cacaatttct tcgtgagttg cctggatcgg gttagtaaag ccgcaggcat
    16801 ctttcagggc attagtcgcc agaaccgcga aatcttcttc tttcacgttc aggtcgcgta
    16861 ggcctgccgg gatatccact ttcttcgcca gttcacggat ggcgttaatg caggcttcag
    16921 caccttccgc gtcgttttta cctgtcacgt tcacgcccat tgcagcggca caatcacgca
    16981 gacgtgcagc ggcgactttg ctgttgaata cctgaacatg cggcagcaaa acggcgttac
    17041 atacaccgtg tggcaggttg tagaaaccgc ccagctggtg cgccatcgca tgaacataac
    17101 ccagagaagc attattgaac gccataccgg cgaggaactg ggcataagcc attgcttcac
    17161 gcgctttcgc attactgcca tcttcaacgg ctaacggcag gttttcggca atcatggtca
    17221 cggctttcag tgcacaagcg tcagtgatcg gcgtggcggc aatagaaaca tacgcttcga
    17281 tagcgtgcgt taaggcatcc atacccgttg cggcggtcag tgacttcggc ataccgatca
    17341 tcagagagga gtcattgaca gaaagcagcg gagtgacatg tttatccaca atcgccattt
    17401 tgatgtgacg cgcttcgtca gtgatgatgc aaaaacgggt catttcagag gccgtacccg
    17461 ccgtggtgtt gatggcgatc atcggcagct gcggttttgc agagcggtca acgccttcat
    17521 aatcgcggat gtcaccgccg ttggctgcaa ccagcgcaat accttttgcg cagtcgtgtg
    17581 gagaaccacc gcctaaggaa atcacgctat cgcaattatt ttctttaagt aatttcaaac
    17641 ctgcagcgac gttttccgtg gtgggattag gttgggtgcc atcataaata acgctaaaaa
    17701 tattgcgttc ttccagtgct ttttgcacat cacccgccat acccaatttc gttaacatat
    17761 tgtcggtgac aattaaggta cgggtaaatc cataatctgc catcatattc attgcatcat
    17821 tcaatgaatc agcaccaata acattcacgg aaggaataaa aaaggttgaa gctgccataa
    17881 cacactcctg aaagtgtaaa aaagggcggg cagcttactg agaattttca tttgaaaata
    17941 tgatcaatat taccgtttat taaaatattt ttaataaaaa gcacgttaca ttcatcaaat
    18001 aacgtgcctt cttaacatta ataagaaatt ttgtattaat tcatatttat tatgaattaa
    18061 tcatttcctt atttttccgg aaataataat gcgtcgcgca ataaatgatc atttccacga
    18121 cgacgcgtaa agccaatgcg gtcaaaatat tccagaatct gaattgccag cttgcggcct
    18181 acgcctaagc gatcgcggaa atccgccgcg caggttgaac cacattcctg atcgagatcg
    18241 cggatcatat tggcaaattc gacaatccga tcgttacggt aataacgatc tttgacgatc
    18301 gcagtaatta tcccctgttg ggccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg
    18361 gttcccgtct cttttgccag gtcacgcacc caccatggct cgtcaccaaa cagcggctct
    18421 gctttttgcc agatggcctg ctgctcttcg ctgaagcccg ctttatgatc cggcagatgc
    18481 agccagccgt gatggctgtg gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt
    18541 accagcgctt catcttccat tggcaacgcc atacgtcgca gacgttcgcg cccaggacca
    18601 ggttcatcgc gatgttgctc atgataagtc gctaatgtgt cgagaatttt ccgctgccag
    18661 cgggcggcaa ctggcgcatt caacaagctg taaccagcct gaatatagcc aggctgttgc
    18721 agcagttcgc gcatcccttc gccgttgagc tgccgtgccc aggcgaaatc cgcaaggtta
    18781 accgcgccgc gttccagatg aacagataac gcatcggcat cgctctgcgc ccgtgcaaga
    18841 gacgccagcc attgcagata ttccggctta cgcttaccgc gacgcggcgg gttaagcatc
    18901 accacacgcg cccccgccag cgtgttgcgg gccgagatat cgcgcagcac tatgcgatcg
    18961 ttatccgcca gccacagcgg cgtgtcgaac accagctcgg caaggttatc ttccagtagc
    19021 gaaacacgtc ccgtaacgtg gctggcggcg tggtgaatat gcagcggctg ccactgggtc
    19081 agcggtgtat gggtttgaag ctcgacaatc acccgtgtga acggctccgg cggcacatcg
    19141 gcaagcagcc agtcgccacg gttaatctgc tctttttccg catcacccgc gatgttaagc
    19201 gcgatacgct gcccggcatg ggcagtttct gttggctgat tttgcgcatg cagcgcacgc
    19261 acgcgcatcg gtttatttac accagtcagc cagagtgaat cgcctacttt cacttccccg
    19321 cttaacgccg taccggtgac gaccagcccg gcacctttta cggtaaatgc gcggtcgatc
    19381 gcgaggcgga aactatgttg gctggcgtgt tcacgttctg gtaactgaag cagatgctcg
    19441 cgcagggcat caattccccg accttcggtt gctgcggtga taaacagttt tgcctcagca
    19501 aaaccgtatt cccgcagcac ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg
    19561 tccacgcgat cggctttggt cagcgccact gtcagcatcg ggttaccggt cagctgcaaa
    19621 atcgccagat gctcacgggt ctgtgccatc acaccgtcat cgcacgccac caccaacagc
    19681 gcgtgatcga taccaccaac gcccgccagc atgttggaaa gaaacttttc atgaccgggt
    19741 acgtcgataa aaccaggcac gcgaccatcc ggctgcggcc agtaggcata gccgagatcg
    19801 atggtcatgc cgcgcttttt ttcttccggc agacggtcgg catttacgcc cgtaatcgcc
    19861 tgcaacaagg ttgttttgcc gtggtcaacg tgtccggcag tcgtaataat catttcaaca
    19921 acatctccaa aaaccgttgc tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt
    19981 cataaatacg accaatcacc ggcactggca attcacgcca gcgggcggct aatgactcaa
    20041 ggtggctacc gcgtccatca tggggggtaa acgttaatgc cgcgctcggc aggcgatcaa
    20101 ccggcagcga accactgccg atctgcgaaa gacatggcat aacctgtacc gcaaactccg
    20161 cgccgtaatg tgcggcaagg agagcctgta aacgttgtgc ctggatttga atgacctctg
    20221 cgctgcgggt aagcaggcgc agggtcggta atttttcact cagagcttca gggtgtaaat
    20281 aaagacgcaa cgtggcttcc agcgccgcga gggtcatttt atccgcgcgt aatgcacgct
    20341 tcagcgggtg gctttgcagg cgggcgatca tttctttttt accaacaata attcctgcct
    20401 gcggcccgcc taacagcttg tcgccggaga agctcaccag actgacgccc gccgcaatca
    20461 actcctgcgg cattggctct ttcggcaaac cgtactggct aagatcgacc agcgagccac
    20521 tgcctaaatc agtcaccact ggaacatcca gttctttgcc gagcgccacc agttccgctt
    20581 catctatcgc tttggtgaac ccctgaatgc tgtagttact ggtatgtact ttcatcaaca
    20641 gtgcggtatt ttcattcacc gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc
    20701 ctacttcgtg tagggtgcat cctgcctgac gcataacatc gggaatacga aacgcgccgc
    20761 caatctccac cagttcgccg cgagaaacca ccacctcttt cccgctggca gtggccgcca
    20821 acatcaataa caccgccgcc gcattgttat taacgataca ggcatcttcc gcccccgtaa
    20881 tacggcacag cagctgcgcc agcgcccgat cgcgatgtcc acgtccggcg tcgtccagat
    20941 cgtactcgag ggtcactggc gaacgcatag ccttcgtaac ggcttccacc gcggcttccg
    21001 cctgtaaagc tcgcccaagg ttggtatgca gcacggttcc cgtcaggttg atcaccggac
    21061 gcagcgcgct ttgcgcctct ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac
    21121 accacgcggg cagcgtctgg ctgtcacgaa tcacttctcg cgcttcgtca agcatctgac
    21181 gcagcaattc caccacgcgg gtgtgaccat aagtatcacg caaagaaagg aaggagctat
    21241 cgcgcaataa gcgatcaata gccggaagtt gactatagag ggaacgcgtt tcggttgtca
    21301 taggaaacct ggctgatcaa ggccctctca cacggagaag ggcgtttaac ataaccacgg
    21361 attgtaacgt gagatgggtc aggaggacat atcgcgcatc aagcctttgg cggttcggtg
    21421 cgagcaaaac tttcgcggct aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg
    21481 acacaccagc ctggcgcaac gcggcggaaa ttgagatatc cgaccgcaca ggcaatggcg
    21541 atagtcgcca gattgaccgt atcggttttg agcgtgccat cgaccagata tccttccagc
    21601 acatccagac tgcggttgat tttctcacgc tgacgtaaca attcatcttc agactgctgc
    21661 gccgccggac gcgcctgttc acgcaccgat accagcccgg catccataat gccatccgcc
    21721 agcgcctcaa ttttgcgcac ccgcaacgac tccagcggat cacgcggcaa catcgccgga
    21781 gcaacattca ttaattcaat atattcagcg atgatcggcg aatcaaacca gcattcgccc
    21841 tcttcggtca ccagcaccgg cacttttcct aacgggttaa attgcgccac gccgttgtcc
    21901 gcgttatagg gcagttcatt aataaattcg aacgttatgc ccttttctaa caacagaata
    21961 gaaagtttgc gtacaaacgg gctggtgtag ctaccgacga gtttcatgcc gagtcctttg
    22021 tgcgaggaaa aatatcagta tggctcaggc tcgcgcaaaa cacaggtgat acaagtctca
    22081 atgatccaaa taaagataat gcatccaact ggtcattctt agcagcactt tccgcatcac
    22141 cgaaacatgg ctgaaatggt cggagtaaac tgccacctgg gcgtagatgc cgtcgggtaa
    22201 ggcatcgata tcagcgttag ggtccagctc aatggttccc agcacaccgt ccgtgccggg
    22261 cacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg gcacgacagg
    22321 taaaatactg gtcagtttgc cgtgaaacac ctgcccaggt agcgcgttaa acaccacttc
    22381 cgcatcatcg ccgggtttca gacgtaacag cgagttttgc cgaaattggg cgacaatttg
    22441 ccgtttttgc tcggggatga agaccatcac cggacgcagc ggcaaggcag cggcgtatgt
    22501 acctgggcgg atcagtacct gagtgacgta gccattgctc ggtgcgcgaa tgacggtctg
    22561 ctcaaggtta tattttgctt cagtaagttg cgctcttaag ctcacaatct gcgattgctc
    22621 gccgttaacc atactgtcga gctggctctg gatctgcgcc tgctccgcca ccgagccttt
    22681 caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa
    22741 cggattcacc gccgcctggc tgcctttcag ataacgttga taatttttaa acagacggtc
    22801 gcgctccgct gaaacctggg tggtgttggc ctgcgcttca gtgagttgcg cgcgcagcgt
    22861 ctttatatta tgcgtcgccg tcatcaggtc tgcctgaagt ctgtcaactc gcgcctggta
    22921 acgaaccggc tcgagcttaa aaagcacctc gcccttctga ataagctgat tatttttgtc
    22981 ggtgacttca gtaacaattc ccgtcacctg tggcgtgatg gggatcgcta tcactgcctt
    23041 ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac aagatcaaac cactcaccag
    23101 aaacacgcct cccagcgccg ccgtcgccag cgtccactga tttaccggaa tgcggaagat
    23161 tttaaagacc gcccacgcca gcgccacgta agttaaaaca atcaatagat ccatatttag
    23221 atctccggaa acgtggaata gtcagtcttt ttctcggcgg cgagttgatg ctccagccgg
    23281 gaaattcggt cagaaagtgc ggcgatttcc gggtcagtcg ctttctcttg cgacgcaaca
    23341 tgtgactgca tgccccagcc gcgctccggt tgatagagcg tcgcccagat ccacaggaac
    23401 ggccagataa catgtagagt aaacaggctc acccagcccg ccgtatgaat agcgtcggca
    23461 tggggatggt tgcgcttttt ggcaatcaga taagggatgt catgtatcgc gatgattcca
    23521 taaaaaatca ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata
    23581 tctgcctcgg attcacgttt atcagtgttg ttttttggct ggcagccaga agggagtcag
    23641 gctgatattt tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt
    23701 taagaggtga tgagttgctt agttacataa cgatttcatg acgaaggcat aacatgctgt
    23761 agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagattcaa
    23821 caaaccatca aaacaaaaat gtgacattac tcacatttaa atgccatttt tagcgaaaat
    23881 cgccgccttg ttgctttttt acacaagcat tttgtgatga gcatcacgtc aattacctct
    23941 ctacccccta catttatgtg actgatatca cacaaaaggc cgccgactgg acagttaacc
    24001 gattcagtgc cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac
    24061 aaaaaacctc gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc
    24121 atccgatatt aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc
    24181 aaatatcggc gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg
    24241 gttaccgaac gaaacgctgg cgaagctggt cgggccgatg atcacttatc tcctgccgct
    24301 gctgatcggt tataccggtg gtaagctggt tggcggcgaa cgtggcggcg tagtcggtgc
    24361 cattaccacc atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gctctatgat
    24421 tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat
    24481 caaatccggt tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct
    24541 cgctattctg gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc
    24601 ggcgggcgtt aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga
    24661 accggcgaaa atcctgttcc tcaacaacgc cattaaccac ggtatcttct cgccgctggg
    24721 tattcagcag tcccatgaac tgggtaaatc catcttcttc ctgattgaag ctaacccagg
    24781 tccaggtatg ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg ctaaacagtc
    24841 tgcgggcggt gcggcaatca tccacttcct ggggggtatc cacgaaatct acttcccgta
    24901 tgtgctgatg aatccgcgtc tgattctggc cgttatcctc ggcggtatga ctggcgtatt
    24961 cacgctgact atcctgggcg gtgggctggt ttctccggca tctccgggtt ctatccttgc
    25021 tgtactggcg atgacgccaa aaggtgctta cttcgctaac atcgcgggtg tgtgtgcggc
    25081 gatggcagtt tccttcgttg tctctgccat tctgctgaaa accagcaaag tgaaagaaga
    25141 agatgatatt gaagctgctg cacgtcgcgt tcaggaaatg aaagctgagt ctaaaggtgc
    25201 gtctccgctg tctgcaggcg atgtgactaa cgacctgagc cacgtccgta aaatcatcgt
    25261 tgcctgtgat gccggtatgg gttccagtgc gatgggtgca ggcgtgctgc gtaagaaaat
    25321 tcaggatgca ggtctgtcgc agatttcggt cactaacagc gcgatcaaca acctgccgcc
    25381 agatgtggac ctggtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc
    25441 gcaggcacag catatttcgc tgaccaactt cctcgacagc ggcctgtaca ccagcctgac
    25501 cgaacgtctg gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga aagacagtct
    25561 gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg agaacatctt
    25621 cctcggtcgc aaagcggcca ccaaagaaga agcgattcgt tttgctggcg agcaactggt
    25681 gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg ctggatcgtg aaaaactgac
    25741 cccgacttat ctgggtgagt ctatcgcggt gccacacggt acggttgaag cgaaagatcg
    25801 cgtactcaaa acgggcgtcg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga
    25861 agaagatgac attgcccgtc tggtgattgg gattgctgcc cgtaacaacg agcacattca
    25921 ggtgatcacc agcctgacca atgcactgga tgatgagtcc gtcatcgagc gtctggcaca
    25981 caccaccagc gtggatgaag tgctggaatt gctggcaggt cgtaagtaat ccattcccac
    26041 cctctccgca tgaagaaggt ggggctaatt gcttgatgcg cttcgcttat caggcctaca
    26101 acataaagca caatttgttt tgtcggtcgg ataaggtgtt tacgccgcat ccggcagtcg
    26161 gtgcacaatg cctgatgcga cgctgacgcg tcttatcaga cctacaggtc agtgccaaac
    26221 acgtaggccg gataaggcgg tttacgccgc atccggcaaa aagcccctct cctcacggag
    26281 agggtttggg tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca ttgatgaagg
    26341 ttaatactat gaaagcatta cattttggcg caggtaatat cggtcgtggc tttatcggta
    26401 aactgctggc agacgcaggt atccaactga cgtttgccga tgtaaatcag gtggtgcttg
    26461 atgccctgaa tgcccgtcat agctatcagg ttcatgtggt tggtgaaacc gagcaggtag
    26521 ataccgtttc tggcgtcgat gccgtcagca gcattggtga tgatgtcgtt gatctgattg
    26581 ctcaggttga tttagtcact accgccgttg gcccggtcgt gctggaacgt attgctccgg
    26641 ccatcgccaa agggctggtg aaacgtaaag aacaaggtaa tgaatctccg ctgaacatca
    26701 tcgcctgtga aaacatggtc cgcggcacca cgcagctgaa aggccatgtg atgaacgccc
    26761 tgcccgaaga cgccaaagcg tgggtagaag aacacgttgg ctttgttgat tccgccgttg
    26821 accgcatcgt accgccttcg gcttcggcaa ctaacgatcc gctggaagtg acggtagaaa
    26881 ccttcagcga atggattgtc gataaaaccc agttcaaagg cacgctgcca aacatccctg
    26941 gcatggaatt aaccgacaat ctgatggcat ttgtcgaacg taaactcttc accctgaata
    27001 caggtcatgc tataaccgcg tacctcggaa aactggcggg tcatcagacc attcgtgacg
    27061 caattctcga cgagaaaatc cgcgcggtgg taaaaggggc aatggaagaa agtggtgcag
    27121 tattgatcaa gcgctacggc tttgacgctg acaagcatgc ggcatacatc cagaaaatcc
    27181 ttggtcgttt cgagaacccg tacctgaaag atgatgtaga acgcgtaggc cgtcagccgc
    27241 tgcgtaaact gagtgctggc gaccgtctga tcaagccact gctcggaacc ctggaatatg
    27301 gtctgccaca taaaaacctg attgaaggta ttgccgctgc aatgcacttc cgcagtgaag
    27361 atgatccgca ggctcaggaa ctggcagcgc tgattgccga caaaggccca caagccgcgc
    27421 tggcacagat ttccggtctt gatgccaaca gcgaggttgt atccgaggcg gtaaccgctt
    27481 ataaagcaat gcaataatgg tggaccaggc gcaggatacc ctgcgcccga ataacagatt
    27541 gtcagatatg caggcaacaa tggaacaaac ccaggccttt gaaaaccgtg tgcttgagcg
    27601 tctgaatgct ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc ttttgaccga
    27661 ggcggtaaat cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg tgaagtatgc
    27721 tgtagaaccg ttactcgacg gcgatggtcc gctgggcgat ctttctgtgc gtttaaaact
    27781 catttacggg ttgggcgtca ttaaccgcca ggaatacgaa gatgcggaac tgctgatggc
    27841 attgcgtgaa gagctaaatc acgacggcaa cgagtacgct tttaccgacg acgaaatcct
    27901 tggacccttt ggtgaactgc attgcgtggc ggcgttacca ccgccgccac agtttgaacc
    27961 tgcagactcc agtttgtatg caatgcaaat tcagcgttac caacaggctg tacgatcaac
    28021 aatggtcctt tcactgacag agctgatttc caaaatcagc ttaaaaaaag cctttcaaaa
    28081 gtaagtactt tcgcttactt ctcctctacc tgtttcggac gataaagctg acgataatac
    28141 tccagacgtt gtagatataa cggcacgctt tcatcaggta ttccggacgg aatcgcgtta
    28201 cggggaggta gttttttcag atactcccgg aacgcctggc tcgatgccat gaaatctacg
    28261 gctttatcga taagaagcgg aacgttttca cctaatttgc ccatgatgat atctccgtat
    28321 taccccgcgc cgggaatgcg cggccgccaa ttttagttta gatcccggta taaatgacat
    28381 taaggaaaac gtgctcaatc ctcaataatc acctgcttct gttgactatc ttttttcttt
    28441 aaatttctta actaatcaat gtattgggtg attttttaac gcattaatgg ttacaatctg
    28501 gaactattac aatgccatca gttgtgaaca aagcacctgg tcgcgcatac tagaggctat
    28561 aaatttatct ctatcagaag tcatcacatg aaagaagtcg aaaaaaacga aatcaaacgt
    28621 ctcagcgatc gtctggacgc catccgtcac cagcaggccg atctatcgct ggttgaagcc
    28681 gcagacaaat atgccgaact ggaaaaagag aaagccacgc tggaagcaga aattgctcgc
    28741 ctgcgcgaag ttcacagcca gaaactcagc aaagaagcgc agaaactgat gaagatgccg
    28801 ttccagcgtg caatcaccaa aaaagagcag gctgatatgg gcaaactgaa aaaaagtgtt
    28861 cgcggactgg tggttgtgca cccaatgacc gcgctgggcc gtgaaatggg cctgcaagag
    28921 atgacagggt tctcaaagac tgcgttttaa gttcctttct cgtcgctgta actactgcca
    28981 ctcacgattt actcgctcct gctggcacat gcaggagcct gtgccactca ttaaatacga
    29041 tgtctttctt accgtaaact gtttcagttt cccctcgagt ttgaggtatt tttgcaggct
    29101 aaaatctgaa aatttgttgc gcaacaacaa gtgatatata aatcataaaa ataaataatc
    29161 gaattatgat gcatgattat ttatctttcc ccataaacac a
//
LOCUS       DAPQZD010000050        28270 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPQZD010000050 DAPQZD010000000
VERSION     DAPQZD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.050"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            375..1145
                     /locus_tag="RO454_002109"
     CDS             375..1145
                     /locus_tag="RO454_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDY0933940.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            1160..2101
                     /locus_tag="RO454_002110"
     CDS             1160..2101
                     /locus_tag="RO454_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDY0933941.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            2152..3438
                     /locus_tag="RO454_002111"
     CDS             2152..3438
                     /locus_tag="RO454_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDY0933942.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3435..3722
                     /gene="fixX"
                     /locus_tag="RO454_002112"
     CDS             3435..3722
                     /gene="fixX"
                     /locus_tag="RO454_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDY0933943.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKRDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3781..5112
                     /locus_tag="RO454_002113"
     CDS             3781..5112
                     /locus_tag="RO454_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0933944.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWTLPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            5220..5750
                     /gene="kefF"
                     /locus_tag="RO454_002114"
     CDS             5220..5750
                     /gene="kefF"
                     /locus_tag="RO454_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDY0933945.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5743..7605
                     /gene="kefC"
                     /locus_tag="RO454_002115"
     CDS             5743..7605
                     /gene="kefC"
                     /locus_tag="RO454_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDY0933946.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRRAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7797..8276
                     /gene="folA"
                     /locus_tag="RO454_002116"
     CDS             7797..8276
                     /gene="folA"
                     /locus_tag="RO454_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDY0933947.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8362..8595
                     /locus_tag="RO454_002117"
     CDS             8362..8595
                     /locus_tag="RO454_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDY0933948.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8598..8882
                     /locus_tag="RO454_002118"
     CDS             8598..8882
                     /locus_tag="RO454_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDY0933949.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIK"
     gene            complement(8909..9757)
                     /gene="apaH"
                     /locus_tag="RO454_002119"
     CDS             complement(8909..9757)
                     /gene="apaH"
                     /locus_tag="RO454_002119"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDY0933950.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(9764..10141)
                     /gene="apaG"
                     /locus_tag="RO454_002120"
     CDS             complement(9764..10141)
                     /gene="apaG"
                     /locus_tag="RO454_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDY0933951.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(10144..10965)
                     /gene="rsmA"
                     /locus_tag="RO454_002121"
     CDS             complement(10144..10965)
                     /gene="rsmA"
                     /locus_tag="RO454_002121"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDY0933952.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVGVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10962..11951)
                     /gene="pdxA"
                     /locus_tag="RO454_002122"
     CDS             complement(10962..11951)
                     /gene="pdxA"
                     /locus_tag="RO454_002122"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDY0933953.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11951..13237)
                     /gene="surA"
                     /locus_tag="RO454_002123"
     CDS             complement(11951..13237)
                     /gene="surA"
                     /locus_tag="RO454_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDY0933954.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(13290..15644)
                     /gene="lptD"
                     /locus_tag="RO454_002124"
     CDS             complement(13290..15644)
                     /gene="lptD"
                     /locus_tag="RO454_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDY0933955.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15899..16714
                     /gene="djlA"
                     /locus_tag="RO454_002125"
     CDS             15899..16714
                     /gene="djlA"
                     /locus_tag="RO454_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDY0933956.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGSGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(16864..17523)
                     /gene="rluA"
                     /locus_tag="RO454_002126"
     CDS             complement(16864..17523)
                     /gene="rluA"
                     /locus_tag="RO454_002126"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDY0933957.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(17535..20441)
                     /gene="rapA"
                     /locus_tag="RO454_002127"
     CDS             complement(17535..20441)
                     /gene="rapA"
                     /locus_tag="RO454_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDY0933958.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(20606..22957)
                     /gene="polB"
                     /locus_tag="RO454_002128"
     CDS             complement(20606..22957)
                     /gene="polB"
                     /locus_tag="RO454_002128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDY0933959.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(23032..23727)
                     /gene="araD"
                     /locus_tag="RO454_002129"
     CDS             complement(23032..23727)
                     /gene="araD"
                     /locus_tag="RO454_002129"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDY0933960.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(23842..25344)
                     /gene="araA"
                     /locus_tag="RO454_002130"
     CDS             complement(23842..25344)
                     /gene="araA"
                     /locus_tag="RO454_002130"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDY0933961.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(25355..27055)
                     /gene="araB"
                     /locus_tag="RO454_002131"
     CDS             complement(25355..27055)
                     /gene="araB"
                     /locus_tag="RO454_002131"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDY0933962.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELCVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRNIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEVWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            27394..28239
                     /gene="araC"
                     /locus_tag="RO454_002132"
     CDS             27394..28239
                     /gene="araC"
                     /locus_tag="RO454_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDY0933963.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWVNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         6311 a         7378 c         7792 g         6789 t
ORIGIN      
        1 ttaatggcta actcataatg ggtattcaat aagctgcatt ctgtgattgt tgtcacattt
       61 ttgtttcggg tgaatagagg gcattttttt gttaattttg attattaatc agtttgttat
      121 gttctgttgt gggtaaaaaa atagcatctg actttcaata ttggtgatcc ataaaacaat
      181 attgaaaatt tctttttgct acgccatgtt ttcaatattg gtgaggagct taacaatatt
      241 gaaagttgga tttatctgcg tgtgacattt tcaatattgg tgattaaagt tttatttcca
      301 aattaaaggg cgtgatatct gtaattaaca ccaccgatat gaacgacgtt tccttcatga
      361 tttctggaga tgcaatgaag attattactt gctataagtg cgtgcctgat gaacaggata
      421 ttgcggtcaa taatgctgat ggttcgttag acttcagcaa agccgatgcc aaaataagcc
      481 aatacgatct caacgctatt gaagcggctt gccagctaaa gcaacaggca gcagaggcgc
      541 aggtgacggc cttaagtgtg ggcggtaaag ccctgaccaa cgccaaaggg cgtaaagatg
      601 tgctatcgcg cggtccggat gaactgattg tggtgattga tgaccagttc gagcaggcac
      661 tgccgcaaca aacggcgagc gtacttgctg cagccgctca gaaagcaggc tttgatttga
      721 tcctctgtgg cgatggttct tccgaccttt atgcccagca ggttggtttg ctggtaggcg
      781 aaatccttaa tattccggca gttaacggcg tcagcaaaat tatctccctg acggcagata
      841 ccctcaccgt tgagcgcgaa ctggaagatg aaacagaaac cttaagcatt ccgctgcctg
      901 cggttgttgc tgtttccact gatatcaact ccccacaaat tccttcgatg aaagccattc
      961 tcggcgcggc gaaaaagcca gtccaggtat ggtcggcggc ggatatcggt tttaacgcag
     1021 aggcagcctg gtcagaacaa caggttgccg cgccgaaaca gcgcgagcgt cagcgcatcg
     1081 tgattgaagg cgacggcgaa gaacagatcg ccgcatttgc tgaaaatctt cgcaaagtca
     1141 tttaattaca ggggatgcta tgaacacgtt ttctcaagtc tgggtattca gcgatacccc
     1201 ttctcgtctg ccggaactga tgaacggtgc gcaggcttta gctaatcaaa tcaacacgtt
     1261 cgtcctcaat gatgccgacg gcgcacaggc aatccagctc ggcgctaatc atgtctggaa
     1321 attaagcggc aaaccagacg agcggatgat cgaagattac gccggtgtca tggctgacac
     1381 tattcgccag cacggcgcag acggtctggt gctgttgcca aacacccgtc gcggcaaatt
     1441 actggcggcg aaactgggtt accgccttaa cgcggcggtg tctaacgatg ccagcgctgt
     1501 tagcgtgcag gacggtaaag cgacagtgaa acacatggtt tacggcggtc tggcgattgg
     1561 cgaagaacgc attgccacgc cgtatgcggt actgaccatc agcagcggca cgttcgatgc
     1621 ggctcagcca gacgcgtcac gcactggcga aacgcacacc gtggagtggc aggctccggc
     1681 tgtggcgatt acccgcacgg caacccaggc gcgtcagagc aacagcgtcg atctcgacaa
     1741 agcccgcctg gtggtcagcg tcggtcgcgg tattggcagc aaagagaaca ttgcgctggc
     1801 agaacagctt tgtaaggcga taggtgcgga gttggcctgt tctcgtccgg tggcggaaaa
     1861 cgaaaaatgg atggagcacg aacgctatgt cggtatctcc aacctgatgc tgaaacctga
     1921 attgtacctg gcggtgggga tctccgggca gatccagcac atggttggcg ctaatgcgtc
     1981 gcaaaccatt ttcgccatca acaaagataa aaatgcgccg atcttccagt acgcggatta
     2041 cggcattgtt ggcgacgccg tgaagatcct tccggcactg accgcagctt tagcgcgttg
     2101 atccactctg gcagggctgc attccggccc tgccgctgac agggagctct tatgtccgaa
     2161 gatatctttg acgccatcat cgtcggtgca gggcttgccg gttcggttgc cgcactggtg
     2221 ctcgcccgcg aaggtgcgca agtgttagtt atcgagcgag gcaattccgc aggtgccaag
     2281 aacgtcactg gcgggcgtct ctatgcccac agtctggaac acattattcc tggtttcgct
     2341 gactccgccc ccgtagaacg cctgatcacc catgaaaaac ttgcgtttat gacggaaaag
     2401 tcagcgatga ctatggacta ctgcaatggc gacgaaacct ctccatccca gcgttcttac
     2461 tccgttttgc gcagtaaatt tgatgcctgg ctgatggagc aggccgaaga agcgggcgcg
     2521 cagttaatta ccgggatccg cgtcgataac ctcgtacagc gcgatggcaa agtcgtcggt
     2581 gtagaagccg atggcgatgt gattgaagcg aaaacggtga tccttgctga tggggtgaat
     2641 tccatccttg ccgaaaagct ggggatggca aaacgcgtta aaccgacgga tgtggcggtt
     2701 ggcgtgaagg aactgatcga gttaccgaag tcggttattg aagaccgttt tcagttgcag
     2761 ggtaatcagg gcgcggcttg cctgtttgcg ggatcaccca ccgatggcct gatgggcggt
     2821 ggcttccttt ataccaatga aaataccctg tcgctggggc tggtttgtgg tctccatcat
     2881 ctgcatgacg caaaaaaatc ggtgccgcaa atgctggaag atttcaaaca acatccggcc
     2941 gttgcaccgc tgatcgcggg cggcaagctg gtggaatatt ccgctcacgt agtgccggaa
     3001 gcaggcatca acatgctgcc ggagctggtc ggtgacggcg tattgattgc cggtgatgct
     3061 gccggaatgt gtatgaacct cggttttacc attcgcggta tggatctggc gattgccgcc
     3121 ggggaagcag cagcaaaaac tgtgctttca gcgatgaaaa gcgacgattt cagtaagcaa
     3181 aaactcgcgg aatatcgtca gcatcttgag agtggcccgc tgcgcgatat gcgcatgtac
     3241 cagaaactac cggcgttcct tgataaccca cgcatgttta acggctaccc ggaactggcg
     3301 gtgggcgtgg cgcgtgacct gttcaccatt gacggcagtg cgccggaact gatgcgcaag
     3361 aaaatcctcc gccacggcaa gaaagtgggc ttcatcaatc tgatcaagga tggcatgaaa
     3421 ggagtgaccg ttttatgact tctcccgtca atgtggacgt caaactgggc gtcaataaat
     3481 tcaatgtcga tgaagagcat ccgcacattg ttgtgaaggc cgatgccgat aaacaggccc
     3541 tggagctgct ggtgaaagcg tgccccgcag gtctgtacaa gaagcgggat gacggcagtg
     3601 tgcgcttcga ttacgccgga tgtctggagt gcggcacctg tcgcattctg gggctggggt
     3661 cggcgctgga acagtgggaa tacccgcgcg gcacctttgg tgtggagttc cgttacggct
     3721 gatgttggtt tgatacgtaa tgccgtactg actctcattg caaaaaaaca ggaataaccc
     3781 atgcaaccgt ccagaaactt tgatgatctt aaattctcct ctattcaccg ccgcattttg
     3841 ctgtggggaa gcggtggtcc gtttctggat ggttatgtac tggtaatgat tggcgtggcg
     3901 ctggagcaac tgactccggc gctgaaactg gacgctgact ggattggctt gctgggcgcg
     3961 ggaacgctcg ccgggctgtt cgttggcaca tcgctgtttg gctatatctc cgataaagtc
     4021 ggacggcgca aaatgttcct cattgatatc atcgccatcg gcgtgatatc ggtggcaacg
     4081 atgtttgtct catcccccgt cgaactgttg gtgatgcggg tacttatcgg cattgtcatt
     4141 ggtgcggatt atcccatcgc cacctcaatg atcaccgagt tctccagtac ccgtcagcgg
     4201 gcgttttcca tcagctttat tgccgcgatg tggtatgtcg gcgcgacctg cgccgatttg
     4261 gtcggctatt ggctttatga tgtggaaggc ggctggcgct ggatgctggg tagcgcagcg
     4321 attccctgtt tgttgatttt gattggtcga ttcgaactgc ctgaatctcc ccgctggttg
     4381 ttacgcaaag ggcgagtaaa agagtgcgaa gagatgatga taaaactgtt tggcgaaccg
     4441 gtggctttcg aggaagagca gccgcagcaa acccgttttc gcgatctgtt taatcgccgc
     4501 cattttcctt ttgttctgtt tgttgccgct atctggacct gccaggtgat ccccatgttc
     4561 gccatttaca cctttggccc gcaaatcgtt ggtttgttgg gattgggggt tggcaaaaac
     4621 gcggcactgg ggaacgtggt gattagcctg ttctttatgc tcggctgtat tccgccgatg
     4681 ctgtggctaa acactgccgg acggcgtcca ttgttgattg gcagctttgc catgatgacg
     4741 ctggcgctgg cggttttggg gctgatcccg gatatgggga tctggctggt agtgatggcc
     4801 tttgcggtgt atgccttttt ctctggcggg ccgggtaatt tgcagtggct ctatcctaat
     4861 gaactcttcc cgacggatat ccgcgcctct gccgtgggcg tgattatgtc cttaagccgt
     4921 attggcacta ttgtatcgac ctggacactg ccgatcttta tcaataatta cggtatcagt
     4981 aacacgatgc taatgggggc gggtatctcg ctatttggct tgttgatttc cgtagcgttt
     5041 gccccggaga ctcgagggat gtcactggcg cagaccagca atatgacgat ccgcgggcag
     5101 agaatggggt aatttgttca gatttctctc ttttctgaat caatattatt gactataagc
     5161 tgcgtgaata tatgactaca ctttgtggga aaacaaaggc gtaatcacgc gggctaccta
     5221 tgattcttat aatttatgcg catccatatc cgcatcattc ccatgcgaat aaacggatgc
     5281 ttgaacaggc aaggacgctg gaaggcgtcg aaattcgctc tctttatcaa ctctatcctg
     5341 acttcaatat cgacattgcc gccgaacagg aggcgctgtc tcgtgccgat ctgatcgtct
     5401 ggcagcaccc gatgcagtgg tacagcattc caccgcttct taaactttgg atcgataaag
     5461 ttttctcgca cggctgggct tacggtcacg gcggcacggc gctgcacggc aaacatttgc
     5521 tgtgggcggt gacgaccggc ggcggggaaa gccattttga gattggtgcg catccgggct
     5581 ttgatgtgct gtcgcagccg ctacaggcga cggcaatcta ctgcgggctg aactggctgc
     5641 caccgtttgc catgcactgc acctttattt gtgacgacga aaccctcgaa gggcaggcgc
     5701 ggcactataa gcaacgtctg ctggaatggc aggaggcaca tcatggatag ccatacgctt
     5761 attcaggcgc tgatttatct cggttcggcg gcgctgattg ttcccattgc ggtacgtctc
     5821 ggtctgggat cggttcttgg ctacctgatc gccggctgca ttattggccc gtgggggctg
     5881 cgactggtga ccgatgccga atctattctg cactttgcag agattggcgt ggtgctgatg
     5941 ctgtttatta tcggcctcga actcgatcca caaaggctgt ggaagctgcg tgcggcagtg
     6001 ttcggcggtg gcgcattgca gatggtgatt tgcggcggcc tgctggggct gttttgtatg
     6061 ttacttgggc tgcgctggca ggtcgcagaa ttgatcggca tgacgctggc gctctcatca
     6121 acggcgattg ccatgcaggc gatgaatgaa cgcaatctga tggtgacgca aatgggccgc
     6181 agagcctttg cggtgctgct gttccaggat atcgcggcga tcccactggt ggcgatgatc
     6241 ccgctactgg cagcgagtag tgcctcgacg acgatgggcg catttgttct ctcggcgtta
     6301 aaagtggcgg gcgcgctggt gctggtggta ttgctggggc gctatgtcac gcgtccggca
     6361 ctgcgttttg ttgcccgctc tggcttgcgg gaagtgttta gtgccgtggc gttattcctc
     6421 gtgtttggct ttggtttgct gctggaagag gtcggcttgt cgatggcgat gggcgcgttt
     6481 ctggcgggcg tactgctggc aagctcggaa taccgtcatg cgctggaaag cgatatcgaa
     6541 ccatttaaag gtttgctgct ggggctgttt ttcatcggcg ttggcatgtc gatagacttt
     6601 ggcacgctgc ttgaaaatcc gttgcgcatt gtcattttgc tgctcggttt cctcatcatc
     6661 aaaatcgcca tgctgtggct gattgcccgg ccgttgcagg tgccaaataa acagcgtcgc
     6721 tggtttgcgg tgctgttagg gcagggcagt gagtttgcct ttgtagtatt tggtgcggcg
     6781 cagatggcga atgtgctgga gccagagtgg gcgaaatcgc tgaccctggc ggtggcactg
     6841 tcgatggcgg caacgccgat tctgctggtg atcctcaatc gccttgagca atcttctact
     6901 gaagaggcgc gtgaagccga tgagatcgac gaagaacagc cgcgggtaat cattgccgga
     6961 ttcggtcgtt ttgggcagat aaccggacgt ttactgctct ccagcggggt gaaaatggtg
     7021 gtgctcgatc acgacccgga ccatatcgaa acgctgcgta aattcggtat gaaagtgttt
     7081 tatggcgatg ccacgcggat ggatttactg gaatctgccg gagcggcgaa agcggaagtg
     7141 ctgattaacg ccatcgacga tccgcaaacc aacctgcaac tgacggagat ggtgaaagaa
     7201 catttcccac atttgcagat tattgcccgc gcccgcgatg tcgaccacta cattcgtttg
     7261 cgtcaggcag gcgttgaaaa gccggagcgt gaaaccttcg aaggtgcgct gaaaaccggg
     7321 cgtctggcac tggaaagttt aggtctgggg ccgtatgaag cgcgagaacg tgccgatgtg
     7381 ttccgccgct ttaatattca gatggtggaa gagatggcaa tggttgagaa cgacaccaaa
     7441 gcccgtgcgg cggtctataa acgcaccagc gcgatgttaa gtgagatcat taccgaagac
     7501 cgtgaacatc tgtcattaat tcaacgacat ggctggcagg gaaccgaaga agggaaacat
     7561 actggcaata tggcggatga accggaaacg aagccctcat cctaataaag agtgacgtaa
     7621 atcacgcttt acagctaact gtttgttttt gtttcattgt aatgcggcga gtccagggag
     7681 agagcgtgga ctcgccagca gaatataaaa ttttcctcaa catcatcctc gcaccagtcg
     7741 acgacggttt acgctttacg tatagtggcg acaatttttt ttatcgggaa atctcaatga
     7801 tcagtctgat tgcggcgtta gcggtagatc gcgttatcgg catggaaaac gccatgccgt
     7861 ggaacctgcc tgccgatctc gcctggttta aacgcaacac tttaaataaa cccgtgatta
     7921 tgggccgcca tacctgggaa tccatcggtc gtccgttgcc aggacgcaaa aatattatcc
     7981 tcagcagtca accgggtacg gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca
     8041 ttgcggcgtg tggtgacgta ccagaaatca tggtgattgg cggcggtcgc gtttatgagc
     8101 agttcctgcc gaaagcgcaa aaactgtatc tgacgcatat cgacgcggaa gtggaaggcg
     8161 acacccattt cccggattac gagccggatg actgggaatc ggtattcagt gaattccacg
     8221 acgccgatgc gcagaactct cacagctatt gctttgagat tctggagcgg cggtaatttc
     8281 gtatattaga attccggcat cactgatacg tgcagatatc cgtgtatact agacgtataa
     8341 attgtacagg agcacgagat catgactgca aaacgtacca cacaaagtgt gaccgtcacc
     8401 gtcgaccgtg agttagtcaa tcgcgctcgt gatgcaggct taaatatgag cgccaccctt
     8461 acggttgcgc tcaatgctga acttaaaaaa catgcagcaa cacgttggcg tgaagagaac
     8521 gcagaagcta tcgctgcgtt aaatcaattg gctgatgaaa ccggatgttt ctctgatgag
     8581 taccggagct tctagcgatg caatttacgg tataccgcag tcgcggcagg aacgccgcgt
     8641 ttccctttgt tattgatgtt accagcgaca ttattggtga gattaatcgc cgtatcgtta
     8701 ttccattaac acctattgaa cgatttagcc gtattcgccc accagaacgg cttaacccaa
     8761 tccttttact gatagacggc aaagaatatg tgctcatgac gcatgagact gcaactgttc
     8821 cagttaacgc gctgggaacg aaattttgcg atgccagcgc gcaccgaacg ttgattaaat
     8881 gagcattaga ttttatgctc gacgggattt aagacgctac cgcctctccc tcactcaaat
     8941 ccttatgccg gttcgacggc tggacaaaat actgtttatc ttcccagcgc aggcaggtta
     9001 atgtaccacc ccagcagcag ccggtatcca gcgcgtaaat accttccggc gtacctttgc
     9061 cttccagcga tgcccagtga ccaaaggcga tgttgtactc ttcagcgacg gggccaggaa
     9121 tcgcaaacca cggtttcagt ggggcagggg cctcttccgg cgattctttg ctgtacatat
     9181 ccagttgacc gttcgggaag caaaaacgca tacgggtaaa ggcgttggtg ataaaacgca
     9241 gtcttcccag cccccgcaat tccggtgacc agttatttgg catatcgccg tacatggcat
     9301 caaggaagaa gggataggag tcactcgaca gcaccgcttc tacatcgcgc gcgcactctt
     9361 tggcggtctg cagatcccac tgcggcgtga tcccggcgtg ggccatcacc aactttttct
     9421 cttcgtcgat ttgcagcaaa ggctggcgac gcagccagtt aagcagctcg tcggcatccg
     9481 gcgcttccag cagcggtgtc aggcgatctt tcggtttatt gcggctgatc ccggcaaata
     9541 ccgccagcag atgcagatcg tgattaccca gcaccagacg tacgctgtcg cctaaggatt
     9601 tcacatagcg cagaacatcc agcgaacccg ggccgcgcgc gaccagatcg cccgtcagcc
     9661 agagggtatc tttcccaggg gtaaattcta ctttatgcag caatgcgatc agttcatcgt
     9721 aacaaccatg aacgtcgcca ataaggtatg tcgccatatt cttttaatga atgagtgtgg
     9781 gaacggcgag tcggaatacg ggaatgtcga tgctgaaagg gacgccattt tcatcgatca
     9841 tttcgtagtg accctgcatg gtgcccagcg gggtttcaat gattgcaccg ctggtgtact
     9901 ggtactcttc gccaggcgcg ataagtggct ggacgccaac cactccttcg ccctggactt
     9961 cggtttcacg gccattgcca ttggtgatca gccagtaacg ccccaacaac tgcactggcg
    10021 ctcgccccag attgcgtatg gttacggtat aagcaaaaac gtaacgttca ttatcaggtg
    10081 aagattgggc ctcaatgtag acgctttgaa cctgaataca cactcggggc gaattgatca
    10141 tcgttaactc tcctgcaaag gcgcgttctc cgccagatag ttcgccatct ggcaatattg
    10201 cgcgacagag atattttccg ctcgcatcgc cgggtcgatc cccattcccg ttaacacccc
    10261 gacgctaaac aggttgccga ggctgttacg aatggtttta cgacgctggt taaaggcttc
    10321 ggtggtgatg cggctcaaca cgcgaacatc tttaaccggg tgaggcatcg ttgcatgagg
    10381 aaccaggcgc acgacggcgg aatccacttt gggtggtggt gtaaaggctg acggcggtac
    10441 ttccagcacc gggatcacgt tgcaatagta ttgcgccatg acgcttaatc gaccatacgc
    10501 cttgctgttc ggtcctgcaa ccagacgatt caccacttct ttttgcaaca taaagtgcat
    10561 gtcggcaatg gcatcagtat agctaaacag atggaacatc aacggcgtgg agatgttata
    10621 aggcaggttg ccgaaaacac gcagcggctg gcccattttc gcggccagtt caccaaagtt
    10681 aaaggtcatc gcatcctgct gataaatcgt cagtttcggg cctaagaatg gatgcgtttg
    10741 cagacgtgcc gccagatcgc ggtcaagttc gatgaccgtc agctggtcca gacgttcgcc
    10801 gaccggttcg gtcaatgccg ccagaccggg gccgatttcg accatcgcct ggcccttctg
    10861 cgggttaatg gcagaaacaa tgctgtcgat cacgaactga tcgttgagaa agttttgccc
    10921 gaagcgttta cgggctaagt ggccctggtg gactcgatta ttcattgggt gttaacaatc
    10981 attttgatgg cgagattaag cgccgtaata aaactgccga catcggcttt accacgtccc
    11041 gccagttcaa gcgcggtgcc gtggtccact gatgtgcgaa taaagggcag gcccagcgta
    11101 atgttcacac cgcgcccgaa gccctggtat tttagcacgg gaagaccctg atcgtggtac
    11161 atcgccagca cggcgtcggc gttatcgaga tatttcggct gaaacagggt atcggcaggc
    11221 agcggcccgt tgagtttcat cccctgttcc cgcagctcgt cgagtaccgg aataatggtg
    11281 tctatctctt ccgtacccat atgaccgcct tcgcccgcgt gcggattcag cccgcagacc
    11341 agaatgcgcg gttcggcaat accaaatttg gtccgcaaat cgtgatgcaa aatagcaatc
    11401 acttcgtgca aaagggcagg ggtgatagca tctgcgatat cgcgcagcgg taaatgcgtc
    11461 gttgccagcg ccacgcgaag ttcttcggtc gccagcatca tcaccacttt tttcgcctgc
    11521 gaacgctctt cgaaaaactc ggtatgaccg gtaaacggaa tgcctgcgtc gttaatgacg
    11581 cctttatgca cggggcctgt gatcagcgca gcaaattcac cgttcagaca gccatcacac
    11641 gctcgcgcca gcgtttccac cacatagtgc ccattttcaa ccgctaacag ccccacagtg
    11701 acagattcac gtagcgcgac aggaagtagc gttaatgtgc ccgcagtttg cggttgtgca
    11761 ggggagttgg gggaataagg gcggagggtg agcggcaaac cgagcatcgc tgcccggtcg
    11821 gtaaggagag tggcatcggc acaaacaacc agttcgaccg gccactcacg ctgtgcaagc
    11881 tggacaatta agtccgggcc aatcccggcg ggctcgccgg gagtgatcac aacacgttgg
    11941 gttttaacca ttagttgctc aggattttaa cgtaggcgct ggcacgttgt tcctgcatcc
    12001 agcttgctgc ttcttccgag aacttacggt tcatcagcat gcggtatgca cgatctttct
    12061 gtgccgcgtc ggttttatcg acattacggg tatccagcag ttcgattaaa tgccagccga
    12121 atgaagagtg aaccggtgca ctcatttgac ctttgttcag gcgggtcagg gcgtcacgga
    12181 aggccggatc gaaaatatct ggtgtagccc agccgagatc accgccctgg ttagcagagc
    12241 ctggatcctg agagaactct ttcgctgcgg cagcaaaagt cgttttacca ctcttgatat
    12301 cggcagcaat ctgttccagt ttcacgcggg cctgttcgtc agtcatgatc ggcgacggtt
    12361 tcagcagaat atggcgagca tgaacttcgg tcaccgagat atttttgctt tcgccgcgca
    12421 ggtcgttaac tttcagaata tggaagccaa cgccggaacg aatcgggcca acaatgtcgc
    12481 ctttcttcgc ggtgcttaat gcctgggcga agatccccgg caactcctga atacggcccc
    12541 agcccatctg gccgccgttc agcgcctgct ggtcggcaga atgagcaatc gccagcttac
    12601 cgaaatcagc gccgttacgc gcctgatcga caatcgcgcg cgcctggctt tccgcttcgt
    12661 tcacctgatc agaggtcggg ttttccggca gcgggatcag gatgtggctc aggttcagct
    12721 cagtgctggc gtcgttttgg ttacccactt gctgcgccag ggattcgact tcctgcggca
    12781 ggatggtgat gcgacgacgc acctcgttgt tacgcacttc agagataatc atctctttgc
    12841 ggatctggtt acgataggtg ttgtagttca gaccatcgta agccagacgg ctgcgcatct
    12901 gatccagcgt catgttgttc tgtttcgcga tgttagcaat cgcctgatcc agctgctcat
    12961 cggagatttt cactcccatt ttctgcccca tctgcaggat aatttgatcc atgatcaaac
    13021 gttccatgat ttggtggcgc agcgtcgcgt catcaggaag ttgttgcctt gcctgagcag
    13081 cgttcagttt taccgactgc attaatccat caacgtcgct ttccagcacg acgccgttat
    13141 tgacgacggc tgcgacttta tcgactacct ggggggcagc gaaactggta ttcgcgatca
    13201 tggcgatacc gagaagcagc gttttccagt tcttcatact ttttccattt caattaaccg
    13261 cactgcggat tacgtggtaa atcaacaaat cacaaagtgt tttgatacgg cagaatgttc
    13321 gaacgcagca tctcttgcgt acccagaccg tagttggagc tcaggccgcg aagttcgatg
    13381 ttaaagccga ttgcgttgtc atataccgca tgttgtttat cgttatccca accgttcagc
    13441 ttccgctcgt aaccgacgcg aatcgcatag cagcaggagc tgtattgcac acctaacata
    13501 gagtcggctt gcttgttcgc attggtgtcg tagtagtagg ccccaacaat ggaccaacga
    13561 tcggcaattg gccagctggc gacggcacct acctgcgaaa taccattctt atattgctca
    13621 gcagtggaat agtacttagg cagcgtagcc tgaatatatt ccgggctggc gtaacggtaa
    13681 ttcagctgta ccagacggtc ttcatcacga cggtattcaa tgctggagtt actggtcgct
    13741 acgttatcca gacgtgtatc gtactgaatc ccgccacgca atccccaacg atcggagatg
    13801 cgccagtaag tatcgcctgc ccataccagt gagcccgttt tgtcgtcatt ctcccatgtt
    13861 atgttgtcat cgccagtgcg agactccgtg aaatagtaga tttgaccaac ggaaatatta
    13921 aaacgttcaa cggcagcatc atcatatatg cgagatgtga caccggtcgt cacctggtta
    13981 gcggaggcaa tacggtcaag accgccgtaa gtccggtccc ggaacaggcc ggagtagtca
    14041 gattgcagca gcgaagagtc gtagttatag attttgctct gatcgcgata tggcacatac
    14101 agatactgcg cgcgtggttc cagtgtctgg gtatagcccg gcgccagcat ttccatatca
    14161 cgctcgaaga ccattctacc gtctaccttg aactgcggca ttacacggtt agcggactct
    14221 gccagcctgg tcgtgtttct ggagttatac cagtcaagat tggtttgctg ataatgggtt
    14281 gccagcaact tcgcttcggt attgatgctg ccccagttat tagagagcgg cagattgatg
    14341 gtcggttcca ggtgaacacg ggttgcttca ggcatgtcgt ctctggtgtt aacaaagtgc
    14401 actgcctgac cgtaaatacg cgtatcaaac ggaccaacat cattctggta gtagttaacg
    14461 tctaactgcg gctctgccga gtagctactg gtgttctgct cgctgaagac ctggaactgc
    14521 ttggttgaaa cggtggcatt gaagttttgc accgcatagc caacgctgaa tttttgcgtt
    14581 gcgtagccgt cggtactgga accgtatttg ttatcgaagt cattgaagta gctaggatcg
    14641 ctgactttgg tgtagtcgac gttgaaacgc cacacctgat ccatgacccc ggagtggttc
    14701 cagtagaaca accaacgacg tgaactgtca tcgttcgggt gttcatcttt atagacttta
    14761 tctgaaggca gatagtccag ttccatcaag ccagcgcccg cctgggagag gtagcggaat
    14821 tcgttctccc acatgatgtt gccacgacga tgcatataat gcggcgtgat ggtggcatcc
    14881 atatttggcg cgatgttcca gtaatatggc aggtagaact caaagtagtt ggtggtggtg
    14941 tacttggcgt tcgggatcaa gaaaccagag cgacgtttgt cacccaccgg caactgcaaa
    15001 tacgggctat aaaagatcgg taccggaccc accttaaagc gggcgttcca gatctccgca
    15061 acttgttctt cgcggtcatg aataatttcg ctacctacca cgctccaggt gtcagaaccc
    15121 ggcagacagg aggtaaagct accgttatcc agaatggtat agcggttttc gccacgttgt
    15181 ttcatcaggt ccgctttacc gcgcccctgg cgacccacca tctggtaatc accttcccag
    15241 acgttggtat ctttggtgtt cagattcgcc cagcctttcg gccctttgag gatcacctgg
    15301 ttatcgtcgt aatggacatt accgagcgca tcaacggtac gtaccggctc cggttgtcct
    15361 ggtgcctctt tttgatggag ctgcacttcg tcggcctgca gacggctgtt accctgcatg
    15421 atatccacgc tgccagtaaa cacggcgtca tccgggtagt cccctttcgc gtggtcagca
    15481 ttgatagtca cgggtaagtc attggtatcg ccctgtacca gaggacggtc atagcttggc
    15541 acgcccaaca tgcactgtga ggcgaggtcg gctgccagtc cctgttgact ataaagggcg
    15601 gtggcaatca tggtggccag gagagtgggg atacgttttt tcatacgttg attttattgt
    15661 tccatcatcg gtaacgttgc gcgtgacaaa cggtcagaga ctaacgtact cgtcatctct
    15721 acgctagtgt taatcctgtc cgaatagcgt cagtggtgtt aggcacggca ttgaatgaca
    15781 ggtatgataa tgcaaattat aggcgatgtc ccacaattga ccgcagccgg aaaacggtaa
    15841 aagcaccttt atattgtggg agatagccct gatatccgtg tgtcgatttg gggaatatat
    15901 gcagtattgg ggaaaaatca ttggcgtggc cgtggcctta ctgatgggcg gcggcttttg
    15961 gggcgtagtg ttaggtctgt taattggcca tatgtttgat aaagcccgta gccgtaaaat
    16021 ggcgtggttc gccaaccagc gtgagcgtca ggcgctgttt tttgccacca cttttgaagt
    16081 gatgggccat ttaaccaaat ccaaaggtcg cgtcacggag gctgatattc atatcgccag
    16141 ccagttgatg gaccgaatga accttcatgg cgcttcccgt actgcggcgc aaaatgcgtt
    16201 ccgggtggga aaatcagata attacccgct gcgcgaaaag atgcgccagt ttcgcagtgt
    16261 ctgctttggt cgttttgact taattcgtat gtttctggag atccagattc aggcggcgtt
    16321 tgctgatggt tcactgcacc cgaatgaacg ggcggtgctg tatgtcattg ccgaagagtt
    16381 aggcatctcc cgcgcccagt ttgaccagtt tttgcgcatg atgcagggcg gtgcacagtt
    16441 tggcggcggt tatcagcagc aatctggcag tggtaactgg cagcaagcgc agcgtggccc
    16501 gacgctggaa gatgcctgta atgtgctggg cgtgaagccg acggatgatg cgaccaccat
    16561 caaacgtgcc taccgtaagc tgatgagtga acaccatccc gataagttgg tggcgaaagg
    16621 tttgccgcct gagatgatgg agatggcgaa gcaaaaagcg caggaaattc agcaggccta
    16681 tgagctgata aagcagcaga aagggtttaa ataagtttca acgtgtaggc ctgataagac
    16741 gcggtaggcc ggataagacg cgccagcgtc gcatccggcg agcagcatca gacaatcggc
    16801 gcacaattgc cggatgtggc gtgaacgcct tatccggcct accgaacttc gccagcgtga
    16861 ctattaaaaa tccgctggtg ctttaaacgt catactattg ccatacgccg gatgggtaat
    16921 cgtcagcatc tctgcatgta gcaacaaacg cggtgccatt gctctcgctt ctggtgatgc
    16981 ataaaaacga tcgccgagaa tcggatgacc cagcgccagc atatgcacac gcagctgatg
    17041 cgaacgcccg gtaatcggtt ttaacaccac tcttgccgtg ttatccgccg catattccac
    17101 cacttcatac tccgtctgcg caggtttacc tgtttcgtaa cagactttct gtttcgggcg
    17161 gtttggccag tcgcaaatca gcggcagatc caccagacct tccgcagggg atggatgccc
    17221 ccagacgcgg gccacatact gttttttcgg ctcgcgctcg cggaactggc gttttaactc
    17281 ccgctccgcg gctttggtca gcgccactac aatcacgccg ctggtagcca tatccagacg
    17341 atgcaccgat tctgcctgcg gataatcacg ctgaatgcgc gtcatcacgc tgtctttgtg
    17401 ctcttccaga cgacccggca cagacaacaa accgctcggc ttgttgacca ccataatatg
    17461 gtcatcctga tacaggacaa ccagccaggg ttcctgcggc ggattgtagt tttccatccc
    17521 catcttcggc tccgttactg atgcgtcaca acgatcaaac gcagggcatc cagacgccag
    17581 cctgcctgat ccaggctttc cattacctgc tgacggttgc tctcaatggc ggtcagttcg
    17641 tcgtcacgaa tgttcgggtt aactgcacgc agcgcttcca gacgagacag ctcggcagac
    17701 agtttttcgt cggcttcgtt acgcgctgca tcaatcaatg cacgggcaga tttctcgatc
    17761 tgcgcttccc ccagctgaag gatagcgtga acatcctgct gcaccgcgtt aaccagcttg
    17821 ctgccggtgt gacggttaac cgcgttaagc tgacggttaa aggtttcaaa ttctacctgc
    17881 gccgccaggt tgttgccgtt tttatccagc agcatacgta ccggcgtcgg tggcaggaag
    17941 cggttgagct gcaactgctt cggagcctgg gcttcaacca cataaatcag ttccaccaac
    18001 agcgtaccta ccggcaacgc tttgtttttc aacagtgaaa tcgtgctgct accggtatcg
    18061 ccagaaagga tcagatccag accgttgcgg atcagcggat gctcccaggt aataaactgt
    18121 gcatcttcac gcgccagcgc cacttcacgg tcgaaggtga tggtgatgcc atcttccgac
    18181 aggccaggga agtccggcac cagcatatga tcggacggcg tcagcacgat catgttgtcg
    18241 ccgcgatcgt cctgattgat accgataata tcgaacaggt tcatggcgaa ggcgatcaga
    18301 ttggtatcgt catcctgctc ttcaatgctt tctgccagtg cctgggcttt ttcgccaccg
    18361 ttggaatgga tctccagcag gcggtcacgc ccctgttcca gttgtgcttt cagcgcttcg
    18421 tgctgctcgc ggcagttttt gattagatcg tcaaagcctt cggtctcatc cggtgaagcc
    18481 agatagttaa tcagatcgtt gtatacgcta tcgtaaatag tgcgtccggt cgggcaggtg
    18541 tgctcgaacg catccagacc ttcgtgatac cagcgcacca gcaccgactg agccgttttt
    18601 tccagataag gcacatggat ctgaatatcg tgcgcctgac cgatacgatc cagacgccca
    18661 atacgctgct ccagcagatc cgggttgaat ggcaggtcaa acatcaccat gtggctggcg
    18721 aactggaagt tacgtccttc agaaccgatt tccgagcaca gcagtacctg cgcaccggtg
    18781 tcttcttcag caaaccaggc ggcagcgcgg tcacgttcga taatcgacat gccttcgtgg
    18841 aacactgcgg cgcgaatacc ttcacgttcg cgcagtacct gctccagttg cagcgcagtg
    18901 gccgctttgg cgcagatcac cagcactttc tgagagcgat gactggtcag gtagcccatc
    18961 agccattcaa cgcgcggatc gaagttccac caggtggcgt tatcgccttc aaattcctga
    19021 taaatacgct ccggatagag catatcgcga gcgcgatctt ccgcactttt gcgtgcgccc
    19081 ataatgccgg agactttaat agcggtctga tactgcgtcg gcagtggcag cttaatagta
    19141 tgcagctcgc gtttcgggaa gcctttcaca ccgttacggg tgttacggaa cagcacgcgg
    19201 ctggtaccgt ggcgatccat cagcatcgaa accagttcct gacgggcgct ctgggcatct
    19261 tcgctgtcgc tgtttgctgc ttgcaacagc ggctcgatat cctgctcgcc gatcatctca
    19321 ccgagcatgt tcagttcgtc attgctgagt ttgttacccg ccagcagcat ggcaacggcg
    19381 tccgcaaccg gacgataatt tttctgctct tcaacgaatt gcgcaaaatc gtggaaacgg
    19441 ttcgggtcca gcagacgcag acgggcgaag tggctttcca tccccagctg ttccggggtt
    19501 gcggtcagca gcagaacgcc cggcacgtgc tctgccagtt gttcaatggc ctgatattca
    19561 cggctcggcg catcttcgct ccacaccagg tgatgcgctt catcgaccac cagtagatcc
    19621 cactcggctt cacagagatg ttccagacgc tgtttgctac gacgggcaaa atccagcgag
    19681 caaatcacca gctgctcggt atcgaacggg ttgtaagcat cgtgctgagc ttcggcataa
    19741 cgctcatcat caaacagagc aaagcgcagg ttgaaacggc gcagcatttc taccagccac
    19801 tgatgctgta aggtttccgg gacgataatc agcacacgtt cagcagcgcc agagagcagt
    19861 tgctgatgca ggatcatccc ggcctcaatg gttttcccta aacccacttc gtcagccaac
    19921 aggacgcgcg gcgcgtggcg gcgaccaaca tcatgagcga tgttgagctg gtgcgggatc
    19981 aggctggtac gctgaccgcg caggccgctg tacggcatac ggaactgttc gctggaatac
    20041 ttacgcgcgc gataacgcag cgcaaagcgg tccatacggt caatctgccc ggcaaacaga
    20101 cggtcctgcg gtttgctgaa caccagtttg ctatcaagga aaacttcacg cagggccacg
    20161 ccggactctt cagtatcaag gcgagtaccg atataggtca gcaagccatt ttcttctttt
    20221 acttcttcga cttgcatctg ccagccgtca tggctggtaa tggtatcacc agggttgaac
    20281 atcacgcggg tcacggggga atcactgcgt gcgtacagac ggttttcacc agtagatggg
    20341 aaaagtaaag tgacagttcg cgcatccacc gcgacaacgg ttccaagtcc caattcgctt
    20401 tctgtatcgc tgatccagcg ttgaccaagt gtaaaaggca tatgtgttcg gctctatatc
    20461 tttaattgca ggcaataacc acccgctacc gtgcttatga ggtagtggtg ttattcaggt
    20521 ccaggaatgg aaagggcgct atggtactgg atggcaaagc attcgtcacg catcaaaatg
    20581 gtatctggcg aactcttttt tttgctcaaa atagcccaag ttgcccggtc ataagtgtag
    20641 caaaattatc ctcaataaaa gggagtattc cctccgctac gggttgtagc tggcgggtca
    20701 gatagtgttc gtaatccagt ggtgaacgtt ggtagtccag cggctccggg ccgttggtgg
    20761 tccatacgta cttaatggtg ccgcggttct gatattgcaa ggggcgaccg cgcttatggt
    20821 tttcttcatc ggcaaggcga gcggcgcgta catgaggcgg cacattacgc tgatattcgc
    20881 tcagcggacg gcgaaggcgt ttacggtaaa ccagccgcgc atccagttca cccgccatca
    20941 gtttgtcgat ggtttcacgc acgtattcct gatatggctc gttgcggaag atacgcaggt
    21001 aaagctcctg ctgaaactgc tgggccagtg gcgtccagtc ggtgcgcacg gtttccagcc
    21061 ctttaaacac catccgctgt ttgtcgccct cctgaatcag tccggcgtag cgctttttgc
    21121 tgccggtatc ggccccgcga atggttggca tcagaaaacg gcagaaatgg gtttcatact
    21181 ccagttccag tgcgctggtc agccgttgtt tttgcagcgt ttccgcccac caggcgttaa
    21241 cgtgctgcac cagtgcacga ccgattttcg ctgcttcttc ttccgaatgt gcacctttca
    21301 gccagacaaa cgttgagtcg gtatcgccgt agataacgtc atagccctgc gcttcaatca
    21361 acgttttggt ttgccgcatg atctgatgac cacgcatggt gatcgacgat gccaggcgtg
    21421 gatcgaagaa acggcaggcg gtggtgccga gcacaccata aaaggcattc ataatgatct
    21481 tcagcgcctg cgaaagcggc ttattaccct gacgtttggc ttcatcgcgc ccgtgccaga
    21541 tgttagtcac aatctccggc aggcaatgtt tctcccgtga gaaccacgca tcgagaaaac
    21601 cttcggtact gtgctctgga tcaggctgcg ccatgccttc caccagcccg acgggatcaa
    21661 tcagaaaggt gcggatgatc gacgggtaca ggcttttgta gtccagcacc agcaccgaat
    21721 cataaagccc aggtcgcgaa tccatcacgt agccgccagg gctggcgtgc ggcggcactt
    21781 cgccgagatt aggcgcgaca taaccagcgc gatgcatacg gggaaaatag agatgaccaa
    21841 acgctgccac cgaaccgccg tgtcgatcca ccggcaggcc gttcaccgtc gcccgttcga
    21901 gcaaaaacgg cataatttca gttttgtgga agatctgcgt caccagctcg caatctttca
    21961 ggttataagt tgccagcgca ggtttatctt cggcgaaacg gcggtcaatt tcgtccattc
    22021 gatcccacgg gttatcgata gattttcctt cgcctaataa ctcctgagcg acggtttcca
    22081 gcgagaatga agagaaattc cagaacgcgg atttcagcgc ctcgataccg tcgataatta
    22141 gccgaccttt aacctgagca aaaaacacac cgtttttaaa gccgtgctcg cgccactcca
    22201 gctcactgtt atcacgcccc agccgcagcg gaatacggta acgctcggca tgtttttgca
    22261 gcattcgcag atcgaactgc accacgttcc aaccgatgat cacatcagga tcgtagttgg
    22321 caaaccaggc gttaagtttt tccagtagca gcgggcggct ggcgacgtat tccagttcaa
    22381 aatcgagcgc ggaggcgtcg ccattctctg gccccagcat ataaacgata cgctgcccgc
    22441 aaccttccag gccgatgcag tacagctcac cgtggcgggt ggtttcaata tcaatagaaa
    22501 cccacttgag cggcggacga tagtcgggat gcggtttcag acgggcatta acgatagcgc
    22561 cattgcgcat atcaccctcg acccacaccg gtgaggtgat aaatcgctcc atcagatagc
    22621 gttctggcgg gcgcacatcg gcctcgtaga cggtaacgcc accttcacgc aggcgctttt
    22681 cgtaattcat caactggcga tgggcgcgac agtaaaggcc atacaccggc tggcggtgaa
    22741 aatcttttaa cgccagcggc gtcaggcgaa agccttgttc accctgcaaa atatgctgag
    22801 cgcggggaac ctgatcggca ggaataaacg ccacggactc ttgcggcgca aggaaaacct
    22861 gcaacggccc gttgtccgtc gccagccaga aggaaacttc ggtcccttgc ggggtgtccc
    22921 gccagtgtcg ggttaagata aaacctgcct gcgccacgct gaaaatccaa taaaaaacca
    22981 ggcttgatta tagcctggtt tcgtttgatt ggctgtggtt ttatacagtc attactgccc
    23041 gtaatatgcc ttcgcgccat gcttacgcag atagtgttta tccagcagcg tttgctgcat
    23101 atccggtaac tgcggcgcta actgacggca gaatataccc atataagcga cctcttccag
    23161 cacgatggca ttatgcaccg catcttcggc atttttgccc catgcaaatg ggccgtgaga
    23221 atggaccagg acgccgggca tttgcgctgc atcgataccc tgtttttcga aggtttctac
    23281 gatgacgtta ccggtttccc actcatattc accgttgatt tctgcgtcgg tcattttgcg
    23341 ggtgcaggga atgggaccgt agaaatagtc ggcgtgggtg gtaccggttg ccggaattga
    23401 ctgacccgcc tgcgcccaga tggtggcgtg gcgcgagtgt gtgtgcacaa tgccgccaat
    23461 ggacgggaat gcctgataga gcagccggtg agttggcgtg tcggaggagg gctttttcgt
    23521 accttcaacc acttcaccgg tttcgatgct gaccacgacc atatcgtcag cggtcatgat
    23581 gctgtagtcg acgccggaag gtttgatcac aaagacgccg cgcccgcgat caacggcgct
    23641 gacgttgccc caggtgagcg tgaccaggtt gtgttttggc agcgccaggt tggcttctaa
    23701 tacctggcgt ttgagatctt ctaacatgtt gactccttcg tgccggatgc gatttgctta
    23761 tccggcctac aaaatcgcag cgtgtaggcc tgataagacg cgtcggcgtc gcatcaggcg
    23821 ttacatacct gatgcggcta cttagcgacg aaatccgtaa tacacttcgt tccagcgcag
    23881 cgcgtcttta aacgctggaa ggcgggtatc gttatcaatc accgtgattt caatgtcgtg
    23941 catctcggcg aactggcgca tatcgttgag gttcagcgca tggctgaaga cggtatggtg
    24001 cgcgccaccg gcaaggatcc acgcttcgga agcggtcggc agatccggtt gcgctttcca
    24061 cagcgcattc gccaccggca gtttcggcag ggagtgcggt gttttcaccg tgtcgatgca
    24121 gttaaccagc agacgataac gatcgccgag atcaatcagg ctggcgacaa tggccggacc
    24181 agtttgagta ttgaagatca ggcgagccgg gtcgtcttta ccgccaatac caaggtgctg
    24241 aacgtcgagg atcggtttct cttccacagc gatcgacggg cagacttcca gcatatggga
    24301 gccgagtacc aggtcattac ctttctcgaa gtgataggtg tagtcctcca taaaggaggt
    24361 gccgccctgc agaccggttg acatcacctt catgatgcga agcagggcgg cagttttcca
    24421 gtcgccttcg cccgcaaagc cgtagccctg ctgcatcaga cgctgtacgg ccagaccagg
    24481 aagctgtttc aggccgtgca aatcttcaaa ggtagtagtg aaagcgtgga agccaccctg
    24541 ttccaggaaa cgcttcatcc ccagctcaat acgcgccgct tccagcacgt tctgtcgttt
    24601 ttcgccgtgg atttgtgtgg caggtgtcat ggtgtagcag ctttcgtact catcgaccag
    24661 cgcgttaaca tcgccgtcgc tgatggagtt caccacctgc accagatcgc caaccgccca
    24721 ggtattgacg gagaaaccaa acttgatctg tgcggcaact ttatcgccat cggtgaccgc
    24781 tacttcacgc atgttatcgc caaaacggca gactttcaga tgacgggtat cctgtttaga
    24841 gaccgcctga cgcatccagg agccgatacg ctcatgtgct tgtttatcct gccagtgacc
    24901 ggtaacaacg gcatgttgct gacgcatacg cgcgccgatg aagccgaact cgcgaccgcc
    24961 atgcgcggtc tggttcaggt tcataaagtc catgtcgatg ctgtcccacg gcagcgccgc
    25021 gttgaactgg gtgtggaatt gcagcaacgg tttgttgagc atggtcaggc cgttgatcca
    25081 cattttggcc ggggagaaag tgtgcagcca caccaccaga ccagcgcaac gatcgtcgta
    25141 attcgcgtcg cggcaaatag cggtgatttc atccggcgtg gtgcccagcg gtttcagcac
    25201 cagtttgcag ggcaacttcg cttctgtatt cagtgcatta acaacgtgtt ccgcatgttg
    25261 cgtcacctgg cgcagagtct ccgggccgta aagatgctgg ctgccaatta caaaccacac
    25321 ttcataatta tcaaaaatcg tcattgtcgt gtccttatag agtcggaacg gcctgggcag
    25381 cctgtgccgg ggcggaagtt ggaagatagt gttgttcggc gctcatcgcc cattgctgat
    25441 agcggcgata aagctgttca aagcgttgtg cctgttcgct gcgcggttgc agggttttct
    25501 ctaccgcact ggccattttt tgctgggctg atgggatgtc tgcgtgcact ttcgcggcga
    25561 cagcagcaaa aatcgccgca ccgagcgcac agcactggtc agaggcaaca atttgcagcg
    25621 ggcgattcag cacgtcgcag caggcctgca taatgacctg gtttttccgc gcgatgccgc
    25681 ccagcgccat cacgttattg acggcgatcc cctgatcggt aaagcactcc atgattgcgc
    25741 gtgcgccaaa ggcggtggca gcaatcaaac cgccgaacag cagcggagcg tcggtagcga
    25801 ggttcagatc ggtaatcacc cctttcaggc gttggttagc gttcggtgtg cggcggccgt
    25861 taaaccagtc gagcaccacc ggtaggtgat ccagagacgg attttgagcc catacttcgg
    25921 tcagcgccgg aagcagttgt ttctggctgg cgttgatttg ctctttcagt tccggatgct
    25981 gggcggcaag ctgttccagc ggccagccga gtacgcgacc aaaccaggcg tagatatccc
    26041 caaacgccga ttggcctgct tccagaccga taaatccagg caccacgctg ccatcaacct
    26101 gaccgcaaat acctttcact gcccgctcgc caacgctctg tttgtcggca atcagaatgt
    26161 cacaggtgga agtaccgata acttttacca gtgcgttagg ctgtgcgcct gcgccaactg
    26221 cgcccatatg gcagtcaaac gcgccgccgg aaatcactac gctttcaggc aggccgagac
    26281 gctgcgccca ttccgggcat aaggtgccca ccggaatatc ggcagtccag gtatcagtga
    26341 acagcgggga aggcaaatgg cgattgagga tcgggtccag ctcatcaaag aaactggctg
    26401 gcggcaggcc accccagctt tcgtgccaca gagatttatg cccggcgctg caacgtccgc
    26461 gacgaatatc ctgcgggcgg gtggtaccgg aaagcagagc tggcacccag tcgcacagct
    26521 caatccatga tgcggcagat tgtgccacgg cgttgtcctg gcgagttaca tgcaggattt
    26581 ttgcccagaa ccattcgctg gaataaatac cgccaatatt gcgggagtag tcaacgttgc
    26641 ccggcgtgtg acataagcgg gtaatctctt ccgcttcttc aaccgcggtg tggtctttcc
    26701 acaatacgaa catcgcgttc gggttctcgg caaactccgg gcgcagcgcc aacacgttgc
    26761 cgtcggcgtc aatcggtgcg ggcgtcgagc cggttgtgtc aacgccaatc ccgaccacag
    26821 ctgcgcgctg ttcgacgcaa agctctgcaa gcacagtttt cagcgccgct tccattgact
    26881 caatgtagtc acgcggatga tgacggaact ggttattcgg ggcatcacaa aattgcccgt
    26941 tctgccaacg gggataccac tctacgctgg tggcgatctc ttcaccagtg gcgcattcca
    27001 ccgccaaagc tcgcacagaa tcactgccaa aatcgaggcc aattgcaatc gccatcgttt
    27061 cactccatcc aaaaaaacgg gtatggagaa acagtagaga gttgcgaaaa aaaacgtcag
    27121 gctggatccg ctaatcttat ggataaaaat gctatggcat agcaaagtgt gacgccgtgc
    27181 aaataatcaa tgtggacttt tctgccgtga ttatagacac ttttattacg cgtttttgtc
    27241 atggctttgg tcccgctttg ttacagaatg cttttaataa gcggggttac cggttgggtc
    27301 agcgagaaga gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata tggacaattg
    27361 gtttcttctc tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg
    27421 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaac
    27481 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc
    27541 accattcgcg gtcagggcat agtgaaaaac cagggacgag aatttgtctg ccgaccgggt
    27601 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcac
    27661 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atgggttaac
    27721 tggccgtcga tatttgccaa taccggtttc tttcgcccgg atgaagcgca ccagccgcat
    27781 ttcagcgacc tgtttgggca aatcattaat gccggacaag gggaagggcg ctattcggaa
    27841 ttgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag
    27901 tcgctccatc cgccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac
    27961 ctggcagaca gtaattttga tatcgccagc gtcgcacagc atgtctgcct gtcgccgtcg
    28021 cgtctgtcgc atttgttccg ccagcagtta gggattagcg tattaagctg gcgcgaggac
    28081 cagcgcatca gtcaggcgaa gctgcttttg agcaccaccc ggatgcctat cgccacagtc
    28141 ggtcgcaatg tcggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc
    28201 ggggccagcc cgagcgagtt tagggctgga tgtgaataat aagaaagaag ggggagtctc
    28261 ttactttcgt
//
LOCUS       DAPQZD010000051        28230 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPQZD010000051 DAPQZD010000000
VERSION     DAPQZD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.051"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="RO454_002133"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="RO454_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDY0933964.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(370..2484)
                     /gene="fusA"
                     /locus_tag="RO454_002134"
     CDS             complement(370..2484)
                     /gene="fusA"
                     /locus_tag="RO454_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDY0933965.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="RO454_002135"
     CDS             complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="RO454_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDY0933966.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="RO454_002136"
     CDS             complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="RO454_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDY0933967.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="RO454_002137"
     CDS             complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="RO454_002137"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDY0933968.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="RO454_002138"
     CDS             complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="RO454_002138"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDY0933969.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="RO454_002139"
     CDS             complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="RO454_002139"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDY0933970.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4687..5409)
                     /locus_tag="RO454_002140"
     CDS             complement(4687..5409)
                     /locus_tag="RO454_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0933971.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="RO454_002141"
     CDS             complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="RO454_002141"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDY0933972.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6609..6827
                     /locus_tag="RO454_002142"
     CDS             6609..6827
                     /locus_tag="RO454_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDY0933973.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="RO454_002143"
     CDS             complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="RO454_002143"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDY0933974.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7561..7761)
                     /locus_tag="RO454_002144"
     CDS             complement(7561..7761)
                     /locus_tag="RO454_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDY0933975.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="RO454_002145"
     CDS             complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="RO454_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDY0933976.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="RO454_002146"
     CDS             complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="RO454_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDY0933977.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10258..12171
                     /locus_tag="RO454_002147"
     CDS             10258..12171
                     /locus_tag="RO454_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0933978.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12171..13193
                     /locus_tag="RO454_002148"
     CDS             12171..13193
                     /locus_tag="RO454_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDY0933979.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13187..13405
                     /locus_tag="RO454_002149"
     CDS             13187..13405
                     /locus_tag="RO454_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDY0933980.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13459..14328
                     /locus_tag="RO454_002150"
     CDS             13459..14328
                     /locus_tag="RO454_002150"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDY0933981.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14383..14787)
                     /locus_tag="RO454_002151"
     CDS             complement(14383..14787)
                     /locus_tag="RO454_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDY0933982.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15089..15721
                     /gene="crp"
                     /locus_tag="RO454_002152"
     CDS             15089..15721
                     /gene="crp"
                     /locus_tag="RO454_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDY0933983.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15772..17862
                     /locus_tag="RO454_002153"
     CDS             15772..17862
                     /locus_tag="RO454_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDY0933984.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="RO454_002154"
     CDS             complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="RO454_002154"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDY0933985.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="RO454_002155"
     CDS             complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="RO454_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDY0933986.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19830..20432)
                     /locus_tag="RO454_002156"
     CDS             complement(19830..20432)
                     /locus_tag="RO454_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDY0933987.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(20422..20589)
                     /locus_tag="RO454_002157"
     CDS             complement(20422..20589)
                     /locus_tag="RO454_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDY0933988.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="RO454_002158"
                     /gene_synonym="rotA"
     CDS             complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="RO454_002158"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDY0933989.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21537..22718
                     /gene="tsgA"
                     /locus_tag="RO454_002159"
     CDS             21537..22718
                     /gene="tsgA"
                     /locus_tag="RO454_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDY0933990.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22980..25523
                     /gene="nirB"
                     /locus_tag="RO454_002160"
     CDS             22980..25523
                     /gene="nirB"
                     /locus_tag="RO454_002160"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDY0933991.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25520..25846
                     /gene="nirD"
                     /locus_tag="RO454_002161"
     CDS             25520..25846
                     /gene="nirD"
                     /locus_tag="RO454_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDY0933992.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25973..26779
                     /gene="nirC"
                     /locus_tag="RO454_002162"
     CDS             25973..26779
                     /gene="nirC"
                     /locus_tag="RO454_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDY0933993.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26798..28171
                     /gene="cobA"
                     /locus_tag="RO454_002163"
     CDS             26798..28171
                     /gene="cobA"
                     /locus_tag="RO454_002163"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDY0933994.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6818 a         7559 c         7142 g         6711 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg
      181 taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg
      241 tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg
      301 gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa
      361 cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc
      421 atcatacttc aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg
      481 agttgcatat ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac
      541 ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc
      601 ggtgttctct tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt
      661 cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga
      721 ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta
      781 gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta
      841 ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga
      901 acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga
      961 ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc
     1021 aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat
     1081 gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt
     1141 ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc
     1201 ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat
     1261 acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt
     1321 cagaccgatt gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt
     1381 agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac
     1441 ggtatcacca gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa
     1501 cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg
     1561 ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg
     1621 ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc
     1681 agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt
     1741 gatttctgct tcagtcagtt cttcaccacc cagatatttt tccatcagct cttcagaagc
     1801 ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc
     1861 cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt
     1921 cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag
     1981 cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc
     2041 acccatgcgg tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg
     2101 acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc
     2161 accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt
     2221 gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt
     2281 agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt
     2341 tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg
     2401 ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg
     2461 tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa
     2521 gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga
     2581 ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg
     2641 tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag
     2701 agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc
     2761 attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg
     2821 agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc
     2881 agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt
     2941 agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa
     3001 cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     3061 ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     3121 cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     3181 acgagcctgc ttacggtctt taacaccgga gcagtcaagc gcaccacgta cggtgtggta
     3241 acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     3301 caagttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     3361 acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     3421 acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     3481 gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt
     3541 agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt
     3601 tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc
     3661 tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg
     3721 ttcgaaattc gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta
     3781 atgggggcat tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca
     3841 tcttgcaata atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc
     3901 gtcagccagg gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca
     3961 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca
     4021 aagggtgtct gcggatctaa cccacgttcg cgcagtgaag ccgcgcaaat ccagcactgt
     4081 tcaatatcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4141 tcgggctttt gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4201 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccggcc tgcagttgta
     4261 ccatgaggcg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4321 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4381 ggtttgacga agcaagcccc agtcttccgg cctccgtttc atcaacaacg ccacggcgta
     4441 atgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4501 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4561 cttcccgata gaaaaaaacg ctgcttaact catggccttc tgctatcagc gcctgcgcaa
     4621 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4681 aacgcattac ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata
     4741 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4801 gaagatccct ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac
     4861 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4921 aacagaagag gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt
     4981 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5041 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5101 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5161 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5221 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5281 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5341 ctggtcaaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5401 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5461 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5521 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5581 ttttagcaga gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5641 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
     5701 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5761 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
     5821 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5881 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     5941 aaaccagacc agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6001 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6061 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct
     6121 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6181 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6241 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6301 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6361 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6421 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6481 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6541 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6601 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6661 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6721 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6781 cgaacatcgc gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag
     6841 cgggaattcc ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg
     6901 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca
     6961 ccgcagcaac cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca
     7021 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7081 ttctgaccag ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7141 atttcaaccg gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt
     7201 tcatcaacgc ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga
     7261 ccgtaagcgt cattcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7321 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7381 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7441 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7501 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7561 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc
     7621 tttgtctgct tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt
     7681 ttcgcgccac atcgccattg aatcctgagc ctgacaggcc gggcattttg cgcccgcaat
     7741 aaaacgtttt cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg
     7801 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7861 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7921 atccaggcgg cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt
     7981 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8041 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg
     8101 caaatgcgga aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt
     8161 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg
     8221 cgtggcgtcg ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat
     8281 atcccgctcc agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8341 accaaaacgc ccaaagccca cgacaataac ctgcggttta tcatcgttga cccatggttt
     8401 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcta ccagcttcat
     8461 cagcagcggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8521 gccctggaat aagcgttgtg aagaagcgga agaaaagagg acaaaggcaa actcaccgcc
     8581 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8641 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca
     8701 caacagatgg gtataaagca ccccgaggtt gagtgacatg ccgacagaga taaagaacaa
     8761 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8821 ttccgccagt agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc
     8881 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     8941 accagaagct gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat
     9001 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9061 acccgccaac aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag
     9121 taccgagaaa cctaactggc cggattcgct gcgattcatc cctttttcac gcatcaactg
     9181 caatgccatt gcggttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9241 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9301 cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag
     9361 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9421 gtcgctaata aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa
     9481 cactgcacca atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa
     9541 cagcactcct gcgagtaaaa aatcagaacc ttccatcagc ggcctcctgg agacaacgga
     9601 tttgccagcc agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt
     9661 ctcgcccagt aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt
     9721 tcaaaggggc gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca
     9781 ctttccggct ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc
     9841 cccggtccgc tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc
     9901 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
     9961 gcctgctcac gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg
    10021 gtaacattgc tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga
    10081 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10141 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10201 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10261 attgtttttt cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10321 gccaccatca accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10381 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10441 ggaagctggc aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg
    10501 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc
    10561 aacgaacgta acgacgggca agccattgca accattcatg gcaagctgga tgctattgac
    10621 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10681 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10741 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10801 ctcgatgccg ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg
    10861 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa
    10921 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    10981 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtagcgca tctgcaaagt
    11041 tatatcgacc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgtattaag
    11101 atgctggagc gtatggagct gattgccccg gcgcacgtcg acaacccgtt ccgctttagc
    11161 ttccgcgcgc cggaaagcct gcctaatccg ttattaaaga tggaaaaagt cagcgcaggc
    11221 tatggtgatc gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt
    11281 ggtctgctag gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11341 cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc
    11401 cagcatcaac ttgaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta
    11461 gcgccgcagg agctggaaca aaaactgcgt gattacctcg gcggctttgg tttccagggc
    11521 gataaagtaa ccgaagaaac gcgccgcttt tccggtgggg aaaaagcccg cctggtgctg
    11581 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11641 gacctcgaca tgcgtcaggc actcactgaa gcattaatcg agttcgaagg cgcgctggtt
    11701 gtcgtctcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11761 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11821 gtacaaaagc aggaaaacca ggccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11881 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt
    11941 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12001 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12061 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12121 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12181 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12241 ttagcaattg tcatctgcaa accatgctgc cgcgtctgtt tcgtcgcaag gtgaaattca
    12301 ccccgcactg gcatcgactg gagttgcccg acggcgattt tgtcgatctc gcatggagtg
    12361 aagatcctgc acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca
    12421 gtctcaatag cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12481 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12541 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc
    12601 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12661 caaaagaagg taatgatctc ccgattgatg cggcggtgat tgtctctgcg ccgtttatgc
    12721 tggaggcctg tagttaccat atggaaaagg gcttttcccg catttatcag cgttacttgc
    12781 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga
    12841 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg
    12901 ccagaattca cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga
    12961 tgctcaatcg gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg
    13021 atcatcaggt gatcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg
    13081 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13141 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13201 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13261 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13321 gctacagtgc ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat
    13381 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag
    13441 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13501 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13561 catgcagccg aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg
    13621 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13681 gacttcggcc ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct
    13741 cgcaaatatc tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc
    13801 accccctggc aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc
    13861 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg
    13921 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    13981 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14041 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca
    14101 aacccgttcg cggcaaaagg tatcccgtca ctggatgaaa gctttgtggt gatccatttt
    14161 cgtaatctgg aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt
    14221 tcccacatca atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt
    14281 atgctgccgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac
    14341 gccggataaa atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc
    14401 atacgagtga gtaatattca ccgctttttc cagcatcagc gccactgagc aatatttctc
    14461 ggcagaaaga tcaaccgcac gcgcaaccgc tgcgtctttc agatcgcgac cggtgacgat
    14521 aaaatgtaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa
    14581 ttttacttca caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat
    14641 ggcactgcaa ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc
    14701 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14761 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14821 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14881 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt
    14941 gatgtactgc atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt
    15001 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15061 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15121 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15181 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15241 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15301 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15361 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15421 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15481 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct
    15541 ggcaaaacaa ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15601 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15661 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15721 atcccgtcag agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15781 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15841 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt
    15901 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta
    15961 atcattggtg cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa
    16021 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16081 ctggggccat tgcacgcaaa attgcttccc gcatcgctgc tcgccgccat ttttaccctc
    16141 agtctggcgg gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg
    16201 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag
    16261 tcactaagtc tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt
    16321 acccagcaca ccgaccctga aaaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg
    16381 gtcgatctaa ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc
    16441 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16501 gtcagtttgc atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa
    16561 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16621 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16681 atcgcccgcc aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16741 attgctcgtg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16801 cagcggcgaa tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg
    16861 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg
    16921 ctgcatctgc cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc
    16981 tatggcgcaa cccgtctgag gattgtaaat cgctccgtag gaaccgtggt cgggttaatc
    17041 attgcaggcg tggcgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg
    17101 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt
    17161 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17221 cttccgcgtc ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg
    17281 ctgtggccgc agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc
    17341 tatcaggacg cgattcgctt gattctgagc gaggacccgc aacctactcc actggcctgg
    17401 cagcgaatgc gggtaaatca ggcgcataat actctgtata actcattgaa tcaggcgatg
    17461 caggaaccgg cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17521 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17581 ccaccggaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17641 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17701 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt
    17761 ggccatctga acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac
    17821 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17881 atgaggtgtt cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acgccccaac
    17941 caccttcgcc accgcgtggg cgaaacgatg catcccttca tcgatatccg catcttccac
    18001 caccagcgac ggcgcaaaac gcatcacatc aggcccggca ttcagcacca ttacgccttc
    18061 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tactgtggtt tcagctctgc
    18121 gccaatcaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18181 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaata ccttccagca cttcaggggt
    18241 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18301 ggaaccgtga gaaccaggat gaaacgcgga agcaatttcc gccgtggtca gcatggcgct
    18361 aatcgggaag ccgccgccta acgctttcgc agaggtcaga atatccggcg tcacgccgta
    18421 gtgcatgtaa gcaaacaaat cgccggtccg ccccatcccg cactgtactt catcaaacac
    18481 caataatgcc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18541 tgccgtcaca ccgccctcac cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18601 catcaccgct ttcactgcgt ggagatcgtt aaagggaacg tggatgatgt ctgccggttt
    18661 tggcccaaag ccatcggaat attttggttg cccgcccacc gagacggtaa acagcgagcg
    18721 accatgaaaa gcgttatgga aggcgataat tttggttttg aacgggctat gacgcacaca
    18781 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18841 caccacgcgt tcggcaaacg ttgcctcaat cagtttacga ccaagacgca gcgccggttc
    18901 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    18961 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19021 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19081 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19141 aattgccatc tcatgatcac cctgttacgc ataaaccaat gtgaaattat aatcacaaaa
    19201 tatgcataaa aaatcactta atagcaatca gaaatcagcg atgcaggaaa ttagccagca
    19261 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ttgcacacct tccagatccc
    19321 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acttcaaagc
    19381 actccggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtgagtggat
    19441 ttgccagccc cttaaatacg cccacaccat tatgtgtaat cggcgaggtt ttgccgtgca
    19501 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg tgccattgcc tggtgaccga
    19561 ggcagacgcc aagaatcggc aaacgcccgg catagtgacg aataacgtca agggagatcc
    19621 cggcttcatc tggcgtacag gggccgggtg agatgacaat tttttgtggt ttaagggcgt
    19681 cgatatccgc caacgtcaac gcatcgttgc gcttaaccaa cacatccgcc cccagttcac
    19741 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atcaataagc aggatcatgg
    19801 cggctccggt acgaaagaac cgcgctattt tactcagatt ccccgacttc gcttaccact
    19861 ttgctaaata tcatctgcag cgcggtgaga tcccccattg ccccattctg atttgcctga
    19921 ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    19981 tgctcgaaga agatccgctg tgccaaacca cttcccaccc ggaagggatg cagcacgttg
    20041 atttcacaat agtaatgcgc caaccgctcg acgaatttat ctttctcaag gctagtcaga
    20101 tacttctctt cctccaaatc ctgcatcagg gcattgccct ctttttcgag ataggcaaag
    20161 tggcagaacg gcgtatctcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20221 aaaatatcct gatacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20281 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20341 cgctgctgct ggcggatgtt gagcttgtta cgcatgatat cgagacccgg gtagagatat
    20401 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagcgctt caaggcgcgc
    20461 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20521 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20581 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20641 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20701 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaacg
    20761 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20821 aataccgcgt aaccgaaatc acgctgaccg tggtcaagga aggcgttatc ggcaacgttg
    20881 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    20941 cgcgtgttgc gcaggccgtt atcagcttca tttttgattg gcgggtttgg ttttttctgc
    21001 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21061 gtcgtgttgt tataaaaacc actgttcaca taatcgacaa agttttgcac agacactggc
    21121 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21181 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21241 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21301 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21361 atctgaaata tatgtaacct gaggataact ttttattccg tgatcaaaat cacctcttaa
    21421 aatgcaattt agcaaccgat tgcaataaaa catttaatca gatcacaaaa tcgcctaaaa
    21481 tcgcccgctc gcggcatagc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21541 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ttcctacgca ctgaccggtg
    21601 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21661 tttccagcat gagtaacacc ttcaccttcc tcaatgccgg cattttaatc tctatcttcc
    21721 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21781 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcggcgg
    21841 cgatgttcat tctcggggtg gtcagcggca tcaccatgtc gattggtaca ttcctggtaa
    21901 cacaaatgta tgaaggacgt cagcgcggtt cacgcctgtt atttaccgac tccttcttca
    21961 gtatggccgg gatgattttc ccaatgatcg ccgcatttct gctggcgcgt agcattgagt
    22021 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    22081 gctgtgagtt cccggcgctg ggcaaacatg cgcccaaaac ggatgctccg gtagagaaag
    22141 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22201 agttaggttt tatctcctgg gtgccggaat atgccaaagg cctgggcatg agcctgaacg
    22261 acgcaggcac gctggtaagt aacttctgga tgtcgtacat ggtcggcatg tgggcattca
    22321 gctttattct tcgcttcttt gatttgcaac gtatcctgac cgtactggct ggtctggctg
    22381 cgattctgat gtacgtcttt aacaccggaa caccggcaca tatggcgtgg tcaattctcg
    22441 ccctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22501 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcgggacc atcggtacta
    22561 tgttgacctt tgtggttacc ggcccgattg ttgaacatag tggtccgcag gcggcgctgc
    22621 tgacggcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22681 gccgtcaccg tcagcataac accctgacct ctcattaatt gttcatgccg ggcggcacta
    22741 tcgtcgtccg gccttttcct ctcttaccct gctacgtgca tctatttcta taaacccgtt
    22801 caatttgtct gttttttgca caaacatgaa atatcagaca attccgtgac ttaagaaaat
    22861 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22921 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    22981 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23041 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23101 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23161 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23221 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23281 tttatgacaa gctgatcatg gcgaccggtt cctacccgtg gatcccgcca atcaaaggtt
    23341 ctgatactca ggactgcttt gtctatcgca ctattgaaga cctcaacgcc attgaatcct
    23401 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23461 ctggcgcgct gaaaaactta ggcattgaaa cccacgttat cgaatttgcc cctatgctga
    23521 tggcggaaca gcttgatcag atgggcggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23581 gcgtgcgcgt tcacaccagc aaaaacactc ttgagattgt gcaggaaggt gttgaagcgc
    23641 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23701 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23761 ggggtatcgt cataaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23821 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23881 aggtcgccgt tgaccatatt cttggtagcg aaaacgcctt tgaaggtgca gaccttagcg
    23941 ccaagctgaa actgctgggc gtagacgtag gcggtattgg tgatgcgcac ggtcgcacgc
    24001 ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga gatctacaaa cgcctgattg
    24061 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24121 gcaacctgct gcaactggtg ctgaacgcta tcgaactgcc ggaaaacccg gattctctga
    24181 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24241 gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga cctgattgct gctatcaaca
    24301 aaggctgcca caccgttgcg gcgctgaaag ccgaaaccaa agccggtacg ggctgcggcg
    24361 gttgtattcc gctggtcact caggtactga acgcggaact ggcgaaacag ggtatcgaag
    24421 ttaacaacaa cctgtgtgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24481 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggttacggtt
    24541 gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24601 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaaa
    24661 aagacggcac ctactcggtg atcccgcgtt ctccgggtgg tgaaatcacc ccggaagggc
    24721 tgatggcagt gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    24781 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggagatctgg cgtcagctga
    24841 ttgaagcagg tttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24901 gcgtgggtag cacctggtgc cgctatggcg ttggcgacag cgtcggcctc ggcgtggaac
    24961 tggaaaatcg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25021 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25081 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25141 cagcggatat cgatcgcgaa acgctgatta aatatcttga ccgcttcatg atgttctaca
    25201 tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggtggca
    25261 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgca catctggaag
    25321 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaataccc
    25381 cgtctgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25441 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25501 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25561 gcctgaaacc ggcgtctgcg cgctgttagg tgacgaacaa gtagcaattt tccgcccgta
    25621 tcacagcgac caggtgtttg cgatcagcaa catcgatccg ttcttcgagt ccagcgtgct
    25681 gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25741 gcagcgtttt cgcttaagcg acggcttgtg catggaagac gaacagtttt ccgtcaagca
    25801 ttacgacgcg cgagtgaaag acggcgtggt gcagctgcgc ggttaatatt ttaacgggag
    25861 gcgcaatgcc tccccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    25921 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    25981 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26041 cccgctcggc ttttgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26101 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggtgc
    26161 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26221 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26281 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtctttgttg ccatgctcta
    26341 tagctggggt ggcggtagcc tgctgccggt cgataccagc atcgttcact ccgtcgcgct
    26401 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26461 ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26521 ctggtggtgt ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26581 gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26641 tcataacctg ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt
    26701 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    26761 tgaaacggct gccggttaat tattaagggg tttttacgtg gatcatttgc ctatattttg
    26821 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    26881 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    26941 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27001 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    27061 cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27121 cgccagcttt attatgccgt cgattattga ccgctcgccg ctcatggtag cggtctcctc
    27181 tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc
    27241 gttacatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27301 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta atgaccgcct
    27361 ggcgcagtcg ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    27421 caatgaaccg ctcgatcatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    27481 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    27541 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    27601 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27661 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    27721 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc
    27781 gggtattacc gcagcctctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    27841 ttatgcccag agcgtacgct taattactgg acacttaaaa accggtggcg agctggactg
    27901 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    27961 aactattcag caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tagcaattgt
    28021 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28081 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28141 cgataaattg aactggttct ctaaccatta atttaaccag ggccagagaa gtctggcctt
    28201 tttcgtttac aactctcttt atatattcag
//
LOCUS       DAPQZD010000052        27316 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPQZD010000052 DAPQZD010000000
VERSION     DAPQZD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.052"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            93..824
                     /gene="artJ"
                     /locus_tag="RO454_002164"
     CDS             93..824
                     /gene="artJ"
                     /locus_tag="RO454_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDY0933995.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(853..1980)
                     /gene="rlmC"
                     /locus_tag="RO454_002165"
     CDS             complement(853..1980)
                     /gene="rlmC"
                     /locus_tag="RO454_002165"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDY0933996.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2021..2509)
                     /locus_tag="RO454_002166"
     CDS             complement(2021..2509)
                     /locus_tag="RO454_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDY0933997.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2569..3414)
                     /gene="potI"
                     /locus_tag="RO454_002167"
     CDS             complement(2569..3414)
                     /gene="potI"
                     /locus_tag="RO454_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDY0933998.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3411..4355)
                     /gene="potH"
                     /locus_tag="RO454_002168"
     CDS             complement(3411..4355)
                     /gene="potH"
                     /locus_tag="RO454_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDY0933999.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            complement(4365..5498)
                     /gene="potG"
                     /locus_tag="RO454_002169"
     CDS             complement(4365..5498)
                     /gene="potG"
                     /locus_tag="RO454_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDY0934000.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5593..6705)
                     /gene="potF"
                     /locus_tag="RO454_002170"
     CDS             complement(5593..6705)
                     /gene="potF"
                     /locus_tag="RO454_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDY0934001.1"
                     /translation="MIALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7055..7531)
                     /locus_tag="RO454_002171"
     CDS             complement(7055..7531)
                     /locus_tag="RO454_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDY0934002.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7619..8521)
                     /gene="rimK"
                     /locus_tag="RO454_002172"
     CDS             complement(7619..8521)
                     /gene="rimK"
                     /locus_tag="RO454_002172"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDY0934003.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8582..9304)
                     /gene="nfsA"
                     /locus_tag="RO454_002173"
     CDS             complement(8582..9304)
                     /gene="nfsA"
                     /locus_tag="RO454_002173"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDY0934004.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9288..9575)
                     /locus_tag="RO454_002174"
     CDS             complement(9288..9575)
                     /locus_tag="RO454_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDY0934005.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9735..9992
                     /gene="grxA"
                     /locus_tag="RO454_002175"
     CDS             9735..9992
                     /gene="grxA"
                     /locus_tag="RO454_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDY0934006.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10022..10399)
                     /locus_tag="RO454_002176"
     CDS             complement(10022..10399)
                     /locus_tag="RO454_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDY0934007.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10668..12353
                     /locus_tag="RO454_002177"
     CDS             10668..12353
                     /locus_tag="RO454_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDY0934008.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12529..13065)
                     /gene="rcdA"
                     /locus_tag="RO454_002178"
     CDS             complement(12529..13065)
                     /gene="rcdA"
                     /locus_tag="RO454_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDY0934009.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            13149..14357
                     /locus_tag="RO454_002179"
     CDS             13149..14357
                     /locus_tag="RO454_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934010.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMVLSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATMHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14357..15172
                     /gene="ybjI"
                     /locus_tag="RO454_002180"
     CDS             14357..15172
                     /gene="ybjI"
                     /locus_tag="RO454_002180"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDY0934011.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            15258..15542
                     /locus_tag="RO454_002181"
     CDS             15258..15542
                     /locus_tag="RO454_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934012.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15583..16815)
                     /gene="mdfA"
                     /locus_tag="RO454_002182"
     CDS             complement(15583..16815)
                     /gene="mdfA"
                     /locus_tag="RO454_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDY0934013.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17100..17696
                     /gene="ybjG"
                     /locus_tag="RO454_002183"
     CDS             17100..17696
                     /gene="ybjG"
                     /locus_tag="RO454_002183"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDY0934014.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17754..18512
                     /gene="deoR"
                     /locus_tag="RO454_002184"
     CDS             17754..18512
                     /gene="deoR"
                     /locus_tag="RO454_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDY0934015.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHISKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18559..19761)
                     /gene="dacC"
                     /locus_tag="RO454_002185"
     CDS             complement(18559..19761)
                     /gene="dacC"
                     /locus_tag="RO454_002185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDY0934016.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20008..20634
                     /gene="gstB"
                     /locus_tag="RO454_002186"
     CDS             20008..20634
                     /gene="gstB"
                     /locus_tag="RO454_002186"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDY0934017.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDTDF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20631..21746)
                     /gene="yliI"
                     /locus_tag="RO454_002187"
     CDS             complement(20631..21746)
                     /gene="yliI"
                     /locus_tag="RO454_002187"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDY0934018.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21857..22240)
                     /gene="bssR"
                     /locus_tag="RO454_002188"
     CDS             complement(21857..22240)
                     /gene="bssR"
                     /locus_tag="RO454_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDY0934019.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22453..23778
                     /gene="rimO"
                     /locus_tag="RO454_002189"
     CDS             22453..23778
                     /gene="rimO"
                     /locus_tag="RO454_002189"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDY0934020.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23825..25153)
                     /locus_tag="RO454_002190"
     CDS             complement(23825..25153)
                     /locus_tag="RO454_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDY0934021.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25161..>27316)
                     /locus_tag="RO454_002191"
     CDS             complement(25161..>27316)
                     /locus_tag="RO454_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDY0934022.1"
                     /translation="LTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYL
                     FSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCF
                     DRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLFLKQGHEI
                     IAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNAS
                     DKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIEALKIREG
                     DAKEIISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQYLAVGII
                     KLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFT
                     RKIATVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNHMFSEFIF
                     CDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCRWVSAERG
                     IISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNE
                     PHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRVSIDDFGT
                     GLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDV
                     ARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7015 a         7042 c         6481 g         6778 t
ORIGIN      
        1 tatttattgc atataaattc acttaatggc attgttatcc catgccgcag acacggccaa
       61 aaatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt gccgctttac
      121 ttgcttcttt tactttcggt gcttctgccg cagagaaaat caattttggc gtttcagcca
      181 cctatccacc ctttgaatct ataggtgcta ataatgagat tgtcggcttt gatatcgacc
      241 tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac ttttactaat cacgctttcg
      301 acagcctgat cccgtcactg aaattcagaa aatatgacgc cgtaatctcc ggaatggata
      361 tcacgccgga gcgtagcaaa caggtatcgt ttaccacgcc ctactacgaa aactcagccg
      421 tcgtgattgc caaaaaagat acctacaaaa cgtttgccga tctgaaaggc aaacgtattg
      481 ggatggaaaa cggcaccacg caccagaaat atattcagga tcagcacccg gaagtgaaaa
      541 ctgtctccta tgacagttat cagaatgcct ttatcgatct gaaaaatggc cgtattgatg
      601 gcgtatttgg tgacaccgca gtggtaaacg aatggctgaa aaccaatccg caactgggcg
      661 ttgctactga aaaagtgacc gatccgcaat acttcggcac cggcctgggt atcgctgtac
      721 gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca attaaagctg
      781 acggtacata tcagaaaatc aatgaccagt ggttcccaca gtaagattca gaaagccgca
      841 cctgcggctt ttttattgct tcaccagtag cgtcagcact tcatagtgcg cggtgtgcgg
      901 gaacatatca aaaagctgta cccgttcgat gcgataacca ggcagttcgc ggatatcttt
      961 cgccatagtt tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag
     1021 ataatcacac agcggtttac cgatgccgcg gcgcggcggg ttaaccagca ccagctccgg
     1081 cacttccccc tgagcggtgg caaactgcgt agagtccagc gcctgaaatt gcaaacgcgt
     1141 taagcccagt tcagcggctg actgcttcgc acaggcaatg gcctctggtg caatttcgat
     1201 cccggttaac tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa
     1261 cagatcccac atatgtttaa ccggcagctg tcgtacccag tcgcgcgcgg tggcgtacag
     1321 ctggctggcg accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac
     1381 gtcattaaaa cgctctgcca gtgcctgttg ttcggtcagg tagatctccg tctccccttc
     1441 cataatcgcc atatgtaccg gctgaatatt gacggtaata actttcagct gcggtaattg
     1501 ttcctgtaac cacggcagcg ccttacgcaa ttgcgccagt ttggtatcag aacgcagtac
     1561 aaaacgcagc atcatgccac catcgctctg gctttcagtc agcagaatgt atttcagttc
     1621 accacgttta cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag
     1681 cgccgcaaaa acgggcgcaa atgaggctgg ataaagcggg cagtcacaaa ggtcttccgg
     1741 tgtaccatct cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat
     1801 tttggcttta ttacgaaacc cttgttccgg gcctgacacc ggcgcacacc attcctcaac
     1861 cggaaaatcg gcgagcagat ttttaagatc ggcagtttta gcggagagtt gctctggaat
     1921 cggctgcgtt atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat
     1981 acattgacct tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc
     2041 gccgactggt cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca
     2101 gcatcaggct atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat
     2161 aaccataccc cagcgaagcc gcccacaagt aacttgcggc tgtgatttgt gtcaacagat
     2221 acagccagcg cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga
     2281 aaaccagcac cagactgctc aaaaagacca gcgttaaact ccaggtttgt acgctgcgat
     2341 gaatgaactc gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc
     2401 cacggatcat aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag
     2461 catgagatga agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc
     2521 cgcgacatgc gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccacgtct
     2581 tgcacgctgg atgtcgcgta tccgctgttt ttctgcgcgc gccatcagat accaggcgat
     2641 aaatccgaca attccgaccg cgccgaggat caatgttgcc agggcgttga tttccggatt
     2701 cacccccatc cgcacgctgg aaaagaccag catcggtaat gtggtggctc ccggcccgga
     2761 aacaaagctg gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga
     2821 aatgatcgcg ggcatgatca tcggtagcgt aatgacaaaa aacactttca gcggcgtcgc
     2881 accgagatcc atcgcggctt cttctatcga gcgatccagt tcccgcagac gcgacgaaat
     2941 aacgactgcc acataagccg tacagaaagt gacatgcgcc agccagatgg tgagcatacc
     3001 gcggtccgca ggccagccaa tagcatgagc aagcgcgacg aataacaaca acagcgacaa
     3061 acccgtgatg acatctggca ttaccagcgg tgcggtgatc atgaaggcaa aaccatttga
     3121 tccgcgaaac ctgccaaagc gcaccagcac gaccgccgca atagtcccga ggatcgccgc
     3181 cgccgtcgcc gcacaggccg caattgtcag acttaaagca accgcactca tcatcgcgtc
     3241 atcgcgcaat aattcgccat accagcgcgt tgaccagccg gcccacaccg tcactagctt
     3301 cgagctgtta aacgaataga taacaagcat cagcattggc gcgtagagaa aggtgaagcc
     3361 caacagcaaa atcacaatcc gccagggcga acgaaccacc ggtaaattat tcatccgtgt
     3421 tctccctcgc ttttttgctg atgtttgtgg aaccacatta tcggcataat tagcagcagc
     3481 aacatgacga tcgctaccgc tgaggccacc ggccagtcgc ggttgttaaa gaactcctgc
     3541 cacagcacgc gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac
     3601 tcgcccacag ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc
     3661 ggcacgatca cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag tgccgcttct
     3721 accagcgaat aatcaatacg aatcaacgcg gtgtaaatcg gcagcaccat aaacggcacg
     3781 taggcgtaaa caatgccaat ataaaccgca aagttggtat gcagaatggt cagcggttga
     3841 tcgataatcc ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc
     3901 atccaggcgt agacacgaat cagaaacgag gtccacgacg gcaggatcac cagtagtaat
     3961 aagatattac gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc
     4021 agtaaacagc aaatagtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa
     4081 tagagcggat cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat agaaagttgc
     4141 ccgtcagccc actccatcaa ttcggtataa ggtggaatag cgcgcgccat ctccgccagg
     4201 cttattttaa agacgatcag aaatggcagc agaaacagca agatcaacca gatatacggc
     4261 aacgcaataa ccagtttgcg cccatgtttc atttgcagct gcgacaacca cagcttaaag
     4321 ggttttggct gggcagcagg ttcaagtgta ctcatctttg ctccttaaac cgtcagcacc
     4381 acacagctgt ccacttccca gcacaaacgc acttcgtcgc cccaggtcgg taacccttta
     4441 cgatgacgat gggcgttttg tagctgggcg ctaatcatct gcccactttt cagacgaacg
     4501 tgatacaccg aaagatcgcc gagataggca atgtgtatca cctcccccac cgcgaagtta
     4561 caaccattgg cgggcggttc ctcgcaaagc atgatttttt ccgggcgaag cgccacatgc
     4621 accggcacgt tatcgaccac tgaagcatcc gcatcgactt tcagtggatg caccagcccc
     4681 ggcgaataaa gcaccagacc atcttcctga cgctctttga gtacaccttc aaagacatta
     4741 accgaaccga taaattcagc actgtagcga gtggtcggat gctcgtagat ctcttccggc
     4801 tcgccaatct ggacaaattt cccacgattc ataatggcga tgcgccccgc catagtcatc
     4861 gcctcttcct gatcgtgagt aaccattacg caggtcacac cgacgcgctc cagaatatcc
     4921 accacttcaa gctgcatccg gtcacgcagc tttttatcca gcgcgcccat cggctcatcg
     4981 agcagtaata gtttcgggcg cttggcaagg cttctggcca gggccacacg ctgacgctga
     5041 ccaccagaaa gctgatgcgg tttgcgtttg gcgaactcct gcatatgcac cagcccaagc
     5101 atctcattga cccggctggc aatttccgct ttcggtagtt tgtcctgttt caggccaaaa
     5161 gcgatgttct gttccacggt catatgcgga aacagcgcgt aagactgaaa catcatattg
     5221 atggggcgca ggtaaggcgg cacctgtgac aaatcgacgc catcgagcat aatctgcccg
     5281 gccgaaggtt gttcaaaacc tgccagcata cgcagcagcg tggacttgcc acagccggat
     5341 gcgcccagca gcgcgaagat ttcacctttg tagatggtca ggctgacatc atccaccgca
     5401 tgttgaccat cgtaggattt ggtcaggtta cggatttcta atagcggcgt cagcgcctta
     5461 cgggttttcg cctgcgggcg agggatagcg tcattcacgg ctgctctccg gcattgaatg
     5521 tgtgcaagcg caccatgatg gtgcgaattg ccggcgggtg tggtgcgccc ctccggcatc
     5581 tacggctgcg gattattttc cgctcttcac tttggtccag gcgcgggtgc gcacacggtc
     5641 gattttcggg tcctgcactt tcagagtgaa cagcttcgca cgaacatccg caggcggata
     5701 aatacccggg ttatcacgga cttccgcact caccagcggc gtggctgctt tattggcgtt
     5761 agcatagaac acatggtcag agatatgtgc caccacatca ggacgcagca ggtaattcag
     5821 gaactgatag gcttcgtctt tgtttttggc atccgcaggc atggcgaata catcaaagaa
     5881 cgccatcgcc ccttcttttg gaatcgagaa cgagacattc acgccattct tcgcttcctt
     5941 cgcgcggttt gacgcctgcc agacatcgcc cgcccagcca atagcgacac agatgtcacc
     6001 attcgccaga tcattgatgt attgggatga atggaaataa cgaatgttcg ggcgcagctt
     6061 taacagcaga tcggtagccg gtccggtgta atcatccgct ttagtgctgt taggatcttt
     6121 gccgagataa ttcagcacgg tagcaaacac ttcttctggc gcatccagga aagagacacc
     6181 gcagcttttc agtttttcca gattttcagg tttgaggatc aagtcccagc tatcgacggg
     6241 cgcgttttcg cccagcaccg ctttaacttt atcgacgtta tagccaatcc cggtcgtcgc
     6301 ccacatatag ggcatagcaa atttattgtc gggatcgtgt ttggcgacca acttcagcag
     6361 ctccggatcg agattcttcc actccggcaa tttgctttta tccagcggct ggaaaactcc
     6421 cgctgtcagc tggcgctcaa gaaagcttgc tgacggcacc accagatcaa agccggtgct
     6481 ccccgccatt aatttgcctt ccagtacttc gttagaatcg aaaacatcat agacgacttt
     6541 aataccggtt tctttttcaa agttggccac cgtgtccggg gcgatataat cagaccagtt
     6601 ataaatgtgg agtgtttttt gttcagccgc gagcgtgctg acagaaacgg ccatcagagc
     6661 acccgcaacc agacccgata gccatttttt atttaaggcg atcatatctc tttccttctg
     6721 aaaattcgtt aacaaacgaa caaaagttgt cacactgaaa gatatgcaat aaacgtgcat
     6781 atttttatcg gcgcggtgta agaaaagaga actctctccc ggcctgcact gctcactata
     6841 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
     6901 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cggcacaaga
     6961 gaagtgaatt aatctttaat aaaaggttaa aatcagcgga taattttgcg gcacgcagtc
     7021 actgcggcag cgactgcgtg ctcgaaagaa gttatcagtg gaggaaatag ttttcggtaa
     7081 cgttattttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
     7141 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
     7201 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
     7261 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggctgataaa tccgccgcca
     7321 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
     7381 tcagatcgat tttagcatca aagacaccgt cgaaattctg catatggggc agatgcagag
     7441 cctgacagtt atcacattca aaaaaactca tccccaggtc atcgagccat tgacgcaacg
     7501 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttc cgataaaaag
     7561 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
     7621 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
     7681 tacccgcgat gtcgataccg gtggtttttt ctattccttc cagccctggc gacgcattca
     7741 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
     7801 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
     7861 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
     7921 tcgcagcgac tacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
     7981 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
     8041 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccagct
     8101 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
     8161 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
     8221 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
     8281 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
     8341 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
     8401 tgttcatata gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
     8461 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
     8521 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcagt
     8581 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
     8641 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
     8701 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
     8761 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttaaga tccggattat
     8821 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
     8881 gttccgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg cccaatccca
     8941 gcgattccgc tgcggttaat gcattctgcg ccatcattgc cgtatcaacg acaccgagca
     9001 acagttgttc cgccaggccg agctgagcat ccggacagat ctgtaaatgg cggttaaagt
     9061 cggcacagaa cacccaaaac tctgccgctt gcgctacgtg tttttgcccg ccggtcagcg
     9121 tcaccagttc ttcacgtaac gctttgtcgg taatgcggat aatgctactg cactgcaaaa
     9181 aactggaact ggacgtcgca cgggcgctgt taataatcgc ctcacgctgc gcttcggaaa
     9241 tgggttcatc agtgaaatgg cgaatggagc gatggccaca aataagttca atggttggcg
     9301 tcattatctt tttctctttc tgaacgtgaa tattgcggtg gatggttcat cagctgtggg
     9361 gcaagacgtt ttgccacctg aagaataacc accaccgcag cgggaagcat gagcaaaaca
     9421 ccgagaaaaa tcatcagaat ctgcacttct ggccgagaaa atggctcagg tagcgacagg
     9481 gagtcgctta ccgacagcag cgccaccgcc agcaacatca ttccgataaa ttccagtatc
     9541 aacacgcttt taggcaattt accgatcgcg cgcatacgct tccctctgca aagtgagcct
     9601 tcagtctaaa acttttcact gtattgtgtt taacagttat agcttttagc aattaatgca
     9661 acaggttaaa cctactttca gcgaatacat tttagcgtga tcattacagg cataaatcta
     9721 tgaggagaga aataatgcaa accgttattt ttggtcgtcc gggttgccct tactgtgtgc
     9781 gtgcaaaaga tctggctgag aaattgagca atgaacgcga tgatttccag tatcagtatg
     9841 tagatattcg tgcggaaggg atcactaaag aagatctaca acaaaaggca ggtaaacccg
     9901 tagaaaccgt gccgcagatt tttgtcgatc agcaacatat cggcggctat accgattttg
     9961 ctgcatgggt gaaagagaat ctggacgcct gatcgtctga caagccctcg cattgagggc
    10021 tttattgatt ttttctgtgc tgtggtttaa acaaactact gataaataag aaacacaatg
    10081 cccccagcgc acaccagaac accgcactta ataaccatgc cagctcttgc cagaatgagc
    10141 gcgtcggtga aaaaaacagc cgcataatga gcatcgagca gggtgccgcc agcattgcgc
    10201 caaacagagg tttcaggact tctctacgat gtgaaaagaa gctggcaact gcaccaggaa
    10261 gaatgaaaaa tagcaaaccg atttcaggat gcccggcagc ccgaaaagcg cctttcatgt
    10321 gcgtcgccag aaaaagacac accacaatga agaggacaaa acagcagatt gcccccgccc
    10381 aacgttgttt atgtttcact cgttcctcct gacactgcgt ctatcgaaca catttttcgc
    10441 cagtgtggcg ttcagtaaga taaagccgct tcgcattcca tgctaatata ggccaacgca
    10501 attcatatag ccgttgatac ctaatgtgat tacactagta aaatatattg ttactttact
    10561 atcgtttagg tgcgctgaat gaatctgcgc cctgaattct ggtaaaaaca ttatcgtaaa
    10621 ttaccatttc tttcaacagc ttactagtaa acaagaagtt agcctccgtg aatataaacg
    10681 tcgccgaatt gttaaatggg aattacattc tgttattatt tgtggtcctc gcgcttgggc
    10741 tatgtctcgg gaaattacga cttggttcga tccaactggg taattccatt ggcgttttag
    10801 tcgtatcgct gttattaggc caacaacatt tcagcattaa caccgacgcg cttaatcttg
    10861 gctttatgct gtttattttc tgcgtcgggg tcgaagccgg accgaacttt ttttccattt
    10921 tttttcgcga tgggaaaaat tacctaatgt tagcactggt gatggttggc agtgcgctgg
    10981 tgatcgcctt agggttaggt aagctgtttg gctgggatat tggcctgacg gccggtatgt
    11041 tagctggctc tatgacgtcg acaccggttc tggtcggtgc tggcgataca ctgcgtcatt
    11101 ccggcatgga aagcaggcag ctctcactgg cactggataa tctgagcctc gggtatgcct
    11161 taacctattt aatcggtctg gtgagtttga ttgttggtgc gcgttacttg ccgaaattgc
    11221 agcatcagga cttacagacc agcgcccagc aaatcgcccg cgaacgtggc ctggacactg
    11281 atgccaaccg taaggtttat ttaccggtga tccgcgccta ccgcgtcggc ccggagctgg
    11341 tggcctggac cgacggcaaa aatctgcgtg aactgggtat ttatcgacaa actggctgct
    11401 acattgaacg tattcgacgt aacgggattc tggcaaatcc agacggtgat gcggtgctac
    11461 aaatgggcga tgaaatagcg ttggtaggct atcccgacgc ccacgcccga ctcgatccca
    11521 gcttccgtaa cggcaaagaa gttttcgatc gtgaccttct cgacatgcgt atcgtcactg
    11581 aagaagtggt cgttaaaaac cataacgccg ttggcaaacg tctcgcacaa ctgaagttga
    11641 ccgatcacgg ttgcttcctt aaccgcgtca ttcgtagcca gattgagatg ccgatagatg
    11701 acaacgtcgt gcttaacaaa ggtgacgttt tacaagtcag cggcgatgcc cgtcgcgtaa
    11761 aaaccatcgc cgatcgcatc ggctttatct cgattcatag ccaggtcact gacttgctgg
    11821 cattctgcgc cttctttgtt attgggctga tgatcgggat gatcaccttc cagttcagca
    11881 cattcagttt cggcatggga aacgctgccg ggttgttatt cgccggaatt atgctgggat
    11941 ttatgcgcgc taaccacccg accttcggtt acattccgca gggtgcatta agcatggtga
    12001 aagagttcgg cttaatggtg tttatggcag gcgttggtct gagcgccggt agcggtatta
    12061 ataacggcct gggcgcgatt ggcggtcaga tgttgattgc cggattgatt gtcagtcttg
    12121 tgccagtggt tatctgtttc ttgttcggtg cttatgtatt gcgaatgaac cgcgcactgt
    12181 tgttcggcgc aatgatgggc gcacgcacct gcgcgccggc aatggagatc atcagtgata
    12241 cagctcgcag taacatcccg gcgctgggct atgcgggcac ctatgcaatc gccaacgtcc
    12301 tgctgacgct ggcagggaca atcatcgtca tggtatggcc aggattagga taaaactgaa
    12361 gttgccctga aaatgaaatt ttttttgcac aaccgcagaa cttttccgca gggcatcagt
    12421 cttaattagt gccactgctt ttctttgatg tccccatttt gtggagccca tcaaccccgc
    12481 catttcggtt caaggttgat gggttttttg ttgcctgaaa tttatctact acccagcaat
    12541 cctctcaacc atcctcaaaa tctcctcgcg tgatagcggc ttacggtcgg tgacaaaatg
    12601 cagcgtcatt ccctcaataa acgcatcaag cgcgcgggcg gttccgggtt caaaccattg
    12661 ttcgagcgtt tgttgactgc gctgcatcca gttttgcatt accgttttta agagcggttt
    12721 tcggctagcc agcgcgtaga gctggtacat cagctccata ttatccggcg ttgcgacctg
    12781 tgagctgtag atcatatcgg tgatagcctg gcatgcgcct ggagcatcac taacatcgct
    12841 aaaaaatgcc tgatattgcc gggacatgat ctcagtaaaa cggctgaacg cctccagcaa
    12901 caactcatca attcctgaaa aatagtaggt catcgatccc aacggcaccc cggcaagggt
    12961 agcaattttg cggtgtgtaa cagcatgtat tccgtaaagt ttcaccgcct ccagcgtggc
    13021 ctggataatt ttttctcgtc gttgcggatc gttagcgcga cgcatgtcat ttcctctctc
    13081 tgcaattgtg tacaaatgta cacagtcttg ctaatgttgt cttccctctt cttattttga
    13141 cgtggtctat gaccgtaaat tcttcacgta atgcattgaa acgccgaacc tgggcgctgt
    13201 ttatgttctt ctttttgcca ggcctgttaa tggcgtcctg ggcaacccgt acgcctgcta
    13261 tccgcgatat tctctctgtc tcgatcgctg aaatgggcgg tgttctcttt ggtctgtcga
    13321 tcggttcgat gagcggtatt ctctgctcgg cgtggttagt gaaacgcttt gggacgcgta
    13381 atgtcatcct tgtcacgatg tcctgcgcat tgatcgggat gatggtatta agtctggcac
    13441 tctggctgac atcgcccttg ctctttgccg ttggtctcgg cgtctttggg gctagttttg
    13501 gttctgcgga agtggcgata aacgttgaag gtgccgccgt tgagagagaa atgaataaga
    13561 cggttttacc gatgatgcac ggtttttata gcctgggcac gctggcaggc gctggtgtcg
    13621 ggatggcact gacggccttt ggcgttccgg caacgatgca cattttattg gcggcgctgg
    13681 taggcatcgc gcctatttat atcgccattc aggcaatccc tgatggtacg ggcaaaaatg
    13741 ctgccgatgg cacccagcat ggcgaaaaag gcgttccttt ttatcgcgat atccagttgc
    13801 tgttgattgg tgttgtagtg ctggcgatgg cctttgccga aggttctgcc aacgactggt
    13861 tacccttatt aatggttgat ggtcacggtt ttagccctac ttccggctcg ctgatttatg
    13921 ccggttttac tctggggatg accgttggac gctttaccgg cggttggttc attgaccgtt
    13981 acagtcgcgt tgccgtggtt cgggccagtg cactaatggg ggcgttgggt attgggctga
    14041 ttatttttgt cgatagcgcc tgggtcgctg gggtgtctgt tgtactttgg ggactgggtg
    14101 cctcgctggg cttcccgctg accatttctg ccgccagcga taccggcccc gatgcaccaa
    14161 cccgcgtcag cgtggtagct acgaccggtt atctggcttt cctcgtcggg ccgccgctgc
    14221 tgggctatct cggcgaacat tatggattac gtagtgcaat gatggttgta ctggcgctgg
    14281 ttattctcgc ggctattgtc gcgaaagccg tcgccaaacc cgataccaaa acgcagacgg
    14341 cgatggagaa tagttgatga gcattaaatt aattgcggta gacatggatg gtactttctt
    14401 aagcgatcaa aaaacctata accgtgagcg gtttatggct cagtatcagc aaatgaaagc
    14461 acaaggcatt cgctttgtgg tcgccagcgg gaatcaatat tatcagttga tctctttctt
    14521 tcctgaaatt gctaatgaaa ttgcctttgt ggctgaaaac ggcggctggg tagtgagcga
    14581 aggcaaagat gtttttaatg gcgagctgtc gaaggatgcg tttactactg tcgtggaaca
    14641 tttgctgacg cgcccggaag tggaaattat tgcctgcgga aaaaatagcg cctataccct
    14701 caaaaaatat gacgatgcca tgaaaacggt ggcggaaatg tattatcacc gtctggaata
    14761 cgtcgataac tttgacaact tagaagatat cttctttaaa tttggcctta atctctctga
    14821 cgaactgatc ccacaagtgc aaaaagcatt acatgaggcc atcggcgata tcatggtacc
    14881 ggtccatacc ggcaacggca gcatcgatct gattatcccc ggtgtacata aagccaatgg
    14941 ccttcgccaa ctgcagaaat tatggggaat agacgacagc gaagtggtgg tctttggcga
    15001 tggcggtaac gatattgaga tgctgcgtca ggcagggttt agctttgcaa tggaaaatgc
    15061 cggcagcgcg gtcgtcgcag cggcaaaata ccgggcaggc tccaataacc gtgaaggcgt
    15121 actggatgtg atcgataaag tgcttaaaca cgaagcgcca tttaagcaat aaaaaaggca
    15181 gcacgcccgg caacacaata cctgaatgaa ggctgcataa ttcgcggaaa ttaacccgct
    15241 gcattatttc aggcgaaatg attatgaaaa attgtctact gttgggcgcg cttttaatgg
    15301 gctttactgg tgtggcgatg gcgcaaagtg tcaccgtcga tgtgccgagt ggatacaaag
    15361 tggttgtggt tcctgattca gtcagcgttc cacaagcggt cagcgtcgcg actgtgccgc
    15421 aaaccgtcta tgtggctcct gcgccagctc cggcttaccg cccgcatcct tatgttcgcc
    15481 atcttgccag cgtaggtgaa gggatggtta tcgaacacca gattgacgac catcaccatt
    15541 aattcgacaa tgcctgatcg cacaaagcaa tcaggcattt ttttaccctt cgtgcgaatt
    15601 tcccatctgt ttatctttta aaaagataac catcagcgac agccacaaaa tgccgttgac
    15661 aaggttgaag agattaaaca gtccgttgcc cccgttcagc caggcatgtt tgctgatttc
    15721 aataccgacg gtaaagatca gcatttgcag catccccatc gccgcagaaa ccgtaccttt
    15781 actcatatcg ctggcaaaca gggttaatcg caccagtccc gcattcgcca gaccaatacc
    15841 gaaagcataa atacttaacc cggcggtcat ccataaatac gcgtgcgatg agataaccgt
    15901 tgccgcagca gcgaccaaca gaccaatcat aatcggccag ccgcccataa taatcagcga
    15961 acgtacggtg cggcgcgagg tcagacgcgc taacagcaag ttacccgcaa ttaacgcccc
    16021 aaaaataggc acttgcagca ggccatattc atagctgctc aactgctcac cggtaataat
    16081 gataatcggc gactgggcga tccacgccag caacggcagg ctaacgaatc ccagcgccag
    16141 cgcccccgcc acaaagcgac cgttcttcag caccagctta tagtcacgac cgagttcttt
    16201 cagcgacagt ttctcgccta tacgcgtggc ggtttcaggc atagctcgtt gcagaccgaa
    16261 aaaggagatc gctgccaatg cggcaaacaa gacaaacatc ccttcccagg gcagcacatg
    16321 gatccacgcc gcgcccacca gcggaccaag tagtggagca atcagcgcca cgttcgccat
    16381 tagcgcggtg atcttgatac aaaccgcctc ttcgaaggat tcctgaattg cggcgtatcc
    16441 cacagcgcca atgaaacaga ggcttatacc ctgcaagaag cgcaacaggg tgaattgttc
    16501 aatgttttgt gccagtaata ttgccagaca ggtgacgata aaccacacca ctcccgccag
    16561 catcaccgga cggcggccaa tacgatccga caacggcccc aggagccatt gtaaaaacat
    16621 cccgcccgcc agatacgcag tcatcgaagt aggaacccaa tcaatgcccg cctgatattg
    16681 ttccaccacg gccaacatac cgggttgaat catatcgttg ccgatatagg ttgaaaattc
    16741 gtaaagcacc agacagagag ggaaaagtaa cgcctgacgt ccaagcctgg caccggaagc
    16801 taatttattt tgcatgcaat ttcttcgcca ataataatcg cgcaaagttt aataaaagcg
    16861 cagctaacga gaaagcgaat tttgtagctg aaaccacggt taagcacatt cttacattat
    16921 tgcgagtata gctacgcttt ctttaagttt tatttaacct ttgcccgtta caatcaccta
    16981 ccgtaaacag gccgcttgag ggaaataaga cgatgccgct ttacccagtt taacctgcac
    17041 tttattctca acgacttgcc tgtattggct cccttttaat cactttgcgt cgggaagtta
    17101 tgctggaaaa tttgaatctc tctctattct ctcttattaa cgcaacgcca gactctgcgc
    17161 cgtggatgat ctcgttggcg atttttattg ctaaagattt gattaccgtg gtgccgttgc
    17221 tggccgcagt actttggttg tgggggctta cagcacaacg gcaactggtg ataaaaatcg
    17281 ctatcgcgct ggcggtcagc ctgtttgtgt cctggacgat gggacatctt tttccgcacg
    17341 accgaccctt tgtcgaaaat atcggctata acttcctgca tcatgcggcg gatgactcat
    17401 tcccgagcga tcacggtacg gtgattttca cctttgcact ggcattttta tgctggcatc
    17461 gcctgtggtc cggctcactt ttaatggtgc tggccgtcgt cattgcctgg tcgcgcgttt
    17521 atcttggcgt ccactggccg ctggatatgc tcggtggatt gctggcaggt atgattggct
    17581 gccttagcgc ccagattatc tggcaagcga tggggcataa actctatcaa cgtctgcaat
    17641 cgtggtatcg cttctgtttt gcattaccga tccgcaaagg ctgggtgcgt gactgatctc
    17701 tgctaaaaag tgtagtattg agcggctcgc ttcaataact attcagaggg attatggaaa
    17761 cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt aaaacgcagc gataagttac
    17821 atcttaaaga cgccgccgcc ctgcttgggg tttcggagat gacgattcgt cgcgatctga
    17881 acaaccacag tgcgcccgtc gttttgctcg gcggctatat tgttctggaa ccacgcagcg
    17941 ccagccatta cctgttaagc gatcaaaaat cccgcctggt ggaagaaaaa cgccgggcgg
    18001 caaaactggc tgcgacgctg gtagaacccg atcagaccct cttttttgac tgtggcacca
    18061 ccacgccgtg gattattgaa gcaattgata atgaaatccc ttttaccgcc gtttgttatt
    18121 cgctaaatac ttttctggcg ctgaaagaga aaccccattg ccgcgcgttt ctttgcggtg
    18181 gtgaatttca cgccagcaac gccattttca aacccatcga ttttcagcaa acactgaata
    18241 atttttgccc ggatatcgct ttttattccg ctgcgggcgt gcatatcagt aaaggcgcta
    18301 cctgttttaa tcttgaagag ttgccggtaa aacactgggc catgtcgatg gcgcaaaagc
    18361 atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg tccggcgcgc atgggtgacc
    18421 tgaaacgctt tgatattgtt gtgagcgatt gttgcccgga agatgagtat gtgaagtacg
    18481 cgcagacgca gcgtattaag ttgatgtatt aatgacgtat aaccggatga cgtttcgcgc
    18541 catccggtta tcagaagatt aagagaacca gctgccgaac cactgatgga atttcatcat
    18601 cacgaaatcc cacatccgac caaagaatcc gccctcttcc acattttcca tcacgatcag
    18661 cggacgctgc tcaatggatt taccgttaag ctggaaatca atggtgccga caacctgacc
    18721 ttttttcagc ggtgcggtaa gctgcggttc cgttaacgta taactcgcct tcaggttttt
    18781 cagctgccca cgcggtatgg tcactgagcc cgcttcgcct gccccgagat tcacttcgct
    18841 cttatcacca aaccagacgc gctgagtcac aaatgtggca tcaggtttaa ttggcgtcac
    18901 ggtttcaaag aagcggaaac cccaggtcaa taatttctca gactcattaa aacggatacg
    18961 gtcagttttc gcccccagca ctacggagat taaacgcata tcgccctggg tagccgaagc
    19021 aaccagatta tatcccgcgc ctgccgtggt tcctgtcttc atgccatcaa cattcagatt
    19081 gctgctccac agcagacgat tacggttagg ctgacgaatt ttgttgaagg tgaactcttt
    19141 ctctttatga atggcgtact cttccggcac atcgtggatc aatgctttac ccagcaatgc
    19201 catatcgcgc gcggtactga actgccccgg cgcatccagg ccgtgcaccg tctggaaggt
    19261 agtgttggtc agacccagtt ttttggcata accattcatc aaaccaataa atgactcctg
    19321 gctcccggca acgtaatcag ccagcgcaat acaggcgtca ttaccggact ggataatcac
    19381 acctttgttc aagtctgcca ccgaaacctg atcgcccggt ttgaggaaca ttaccgatga
    19441 accacgcagt gccggatttc ccgtcgccca ggcatcttta ccgaccgtca ccatatcggt
    19501 gagtttaatt ttatcggcct taagcgcctg cccaaccaca tagctggtca taattttagt
    19561 caggctcgcg ggatccagtt tctcatccgc attgccttct gccagcactt taccgctggc
    19621 gtaatccatt aaaatccatg cacgggcatc cacgctcggc gcttcaacgg tttgttccgc
    19681 cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa cccgctgcaa gaccacgaag
    19741 gagagaggag tattgcgtca taaatgccac ccgagtatcc attcaaaaaa aatacgttat
    19801 atcgccgtat gataagttcg gtgattaata acgcacacac aaacttaaag aaaccgtaag
    19861 ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt cgctgcttct ggtgtcattt
    19921 ttttgccagc tgttgcttaa aagttaactt tacttcacca tcggagtcct gtttagcctg
    19981 tggctgcctg tcggaaaagg ggtgagtatg attacgctgt ggggtcggaa taattcaacc
    20041 aacgtaaaaa aagtattgct gacgctcgaa gaactggaac taccttatga gcaaattctc
    20101 gcgggccgtg agtttgggat aaatcacgat actgattttc tggcgatgaa ccctaacggg
    20161 ctggtgccgt tgttgcgtga cgacgaaagt gatctcattc tttgggaatc aaacgccatt
    20221 gtccgctatc tggcggcaca gtacgggcaa aaacgcctgt ggatcgactc accggcacgt
    20281 cgtgcggaag cagaaaaatg gatggactgg gcaaaccaga cgctcagtaa tgctcatcgc
    20341 gggatcctga tgggattagt cagaacacca ccggaagagc gcgatcaggc cgccattgat
    20401 gccagttgca aagagtgcga cgccctgttt gccctgctcg atgcggaatt ggcaaaagta
    20461 aaatggttct caggcgacga gtttggtgtg ggcgatatcg ctatcgcacc gttcatctac
    20521 aatttgttta acgttggcct gacctggaca ccgcgtccaa atctgcaacg ctggtatcag
    20581 caactcactg aacgccccgc agtgcgcaaa gtggtgatga ttcccgttag ctaattgcgt
    20641 gggctaactt taagtaattc cccactggac tcgtcggtga gaacgtataa ataaccgtcg
    20701 ggtccagtgc gaacatcacg aattcgttgc cctctgtccg ttaaaatacg gccatcttct
    20761 gtcactttgt cgccgttgac gctcatcaca atgacatctt tatctttcag cgcgccaata
    20821 aataattttt gctgccactg ggggaatttg tcgctgttat agaaagccat gccgctcacg
    20881 gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc cggcgacgat ctcccctttc
    20941 gcttccggta tcttaaagcc cgaatagttg attccccagg ttgccagcgg ccagccgtag
    21001 tttttgcctt tttgcggaat attaatttca tcaccaccgc gcgggccatg ttcattcagc
    21061 catagtgcat tactccacgg attcatcgcc attccttgcg gattacgaat gccataagac
    21121 cagatctcgg cgcgcacacc ggattccttt ataaaaggat tatcatccgg gatttctccc
    21181 aggtcggtca gacgcaccag tttgccctgt aatttatcca gatcctgcgc cgtcgggcgc
    21241 tgattgtttt cgcccagagc aataaaaaga taacctttac cgtcaaatac catccgcccg
    21301 ccaaaatggt tgccggtaga cagttttggc atctgacgaa agacggtgcg gaagtcggtc
    21361 acttttgaga gatcatcact taagcgacca taacccacag cagttccggc tttgccatca
    21421 tcgccaactt cggaataact taaccagatg cggcgcgact gagcaaaatc gggcgctaaa
    21481 accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt ccggcactcc ggaaagcggc
    21541 gcagataatc cttttcctgc ttgccagtga cgcaactcgc cgccgcgcag agtgattaac
    21601 ataccgtgat tatcgggtaa aaaggccagc gcccagggat ggtcgagttt gtcttgcagt
    21661 acttcgacat ttaccgttgc aggagccgcc cagagagcgg aagaaagaca aataaggggc
    21721 acaaggaaaa aggattgtcg atgcataacg cctccggatt gactctggct taaagcgtag
    21781 tcagtggagg agagaacaaa ttcattttta caaaaactta aacatgaagg gggagacgct
    21841 ttctccccct tagttttcag gccttctcaa gcatggcgtg cttctgcagg ctctggatac
    21901 tcagcgttaa gctcatcaga caattttcaa gcttatcggc gttgacggta ataacagtcg
    21961 ggcaatcatg gtgcccactc atcaaacata ctgcggctgt cgctaatgct tcttcagcat
    22021 gatgaagagc actccactct tcctgatcca gatgaagatt caaccgcagc gatttatcgt
    22081 gcagttcgcg attcagttta aaaaagttat ctcgtagatg attgctttcg ctgacggaca
    22141 tgtatccttt tgccttcctc agttgcacgt atgcggcgag gtcaacgcgt gcgtcgatca
    22201 accggttcag caggtcgatt cgctgtctgt caacgaacat acttcgatcc tcctctttcc
    22261 agccttgttg caattaaata gtatggctgt tttttgagca aaatgctgtc caggatcaat
    22321 tcttcacgcc gaattaacaa aatttacatt tccttcaact ccgccagcga atgagattgc
    22381 gatacaaatt ttatccagaa ctctgtagaa tccctgccct gacctttcct gtaactgaat
    22441 attttttaag ctatgagcaa agtaactccc cagccgaaaa tcggctttgt ttcccttggc
    22501 tgcccgaaaa accttgtcga ttcagagcgt attctcaccg aactacgcac tgaaggttat
    22561 gacgtggtac cgagctatga cgatgcggac atggtgatcg tcaacacctg cggctttatt
    22621 gacagcgcgg tacaagaatc actggaagcc attggtgaag cgttgaatga aaacggcaag
    22681 gtaattgtga ccggttgtct gggggcaaaa gaagatcaga tccgcgaagt ccacccgaaa
    22741 gtgctggaaa tcaccgggcc tcatagctat gagcaggttc tggagcacgt tcatcactac
    22801 gtgccaaaac cgaaacacaa cccgttcctg agcctggtgc cagaacaagg tgtgaaactg
    22861 acgccgcgtc attatgctta tctgaaaatt tctgaaggct gtaatcaccg ctgcaccttc
    22921 tgcattattc cgtctatgcg cggcgatctg gtgagccgtc cgattggcga agtgttaagt
    22981 gaagcgaaac gtctggttga tgcgggcgtt aaagaaattc tggtgatctc gcaggatact
    23041 tccgcgtatg gcgtcgatgt taaacatcgt actggcttcc acaacggcga gccggtaaaa
    23101 accagcatgg tcagcctgtg cgaacagtta tcgaaactgg ggatctggac acgtctgcac
    23161 tacgtttacc cttatccgca tgtggacgac gtcatcccac tgatggcaga gggcaaaatc
    23221 ctgccgtatc tggacattcc gttgcagcac gccagcccgc gcattctcaa actgatgaag
    23281 cgtccgggtt ctgtagatcg ccaactggcg cgcatcaaac agtggcgcga aatctgcccg
    23341 gaactgaccc tacgctcaac ctttattgtt ggcttccctg gcgagacgga agaagatttc
    23401 cagatgctac tcgacttcct gaaagaagca cgtctggatc gcgttggctg ctttaaatac
    23461 agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc aggttccgga agaagtgaaa
    23521 gaagaacgct ggaaccgttt catgcagctg cagcagcaga tttccgccga gcgcctgcaa
    23581 gagaaagtgg gtcgtgaaat tctggtgatt atcgacgaag tggacgaaga aggcgcgatt
    23641 ggtcgcagca tggcagatgc accggaaatc gacggcgcgg tttatctcaa tggtgaaacc
    23701 aacgttaagc cgggtgatat cctgcgagtg aaagtcgagc acgccgatga gtacgatttg
    23761 tggggtagcc gggtttaatt aattcaggcc gggcaaaatc atttgtccgg cctgcgaata
    23821 atgattactg ccagctaatc tgcgccctgc cgtttttctt actcttatac agccgctcat
    23881 ccgccagcat tttcaatcgt tcgacggtag tacattcatt tgaatgcgct acaccgatac
    23941 tggtgcttaa attaatgctt cccacctgga aatgaacgat ttcttttaga atcgatagca
    24001 actcttccag cgtgccgcca gtataaatca ccgcaaattc gtcacctccg aagcggtaga
    24061 ctttaccttt atctaccggg atctgtgatt taaggatttt caccactttc atcaacactt
    24121 catcgcccag caaatgaccg aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca
    24181 ggcacaaata accgcctttt tccggcagcg cttttaaatc ttcgtcaaag gattttctgc
    24241 ccatcgcttt ggtcaggtcg tccaggaatg ccgagttata catactgtag agttttttga
    24301 catcggcagc cgaaaagaaa agcccgccat ccagtacgcg ttcaaaatgg ttattaataa
    24361 tggcttcttt ctccacaata ccacgattaa tcactctgcg taccagatag aggcaactgg
    24421 cacccgatat cactgccacc aatgcggcaa agaaaataat caatgtacat acctgaaaat
    24481 aaacgtcttt tttataagca aatacgtaca atccggtcgg caaataggag aagtgctcca
    24541 ttgcttccga aacggtgaag atggcgtttt tagcgttata ttcatcaccg gtcaccgatt
    24601 taccgttctt gaacagggag aacatcaggt tctcgctatc catcgccagc gattttgaca
    24661 gtaaagaaaa acgttccaga ccaagcaaaa atatcagact acgattgtga gttaccggat
    24721 agaggtaata gacgaaggtt ttaccatcaa aagagataaa ccctgtattt ttgacatgtc
    24781 gatacttaac aacctgcata atatctgcac aataattttt ctcaaactcc tttacctgaa
    24841 tacattcgtc actggaaatc agataaggac cgacatcgta caaataagag ggtgaaaccg
    24901 agtcggcgta agctgtgcgt aagccaacca acagctgttc ctccgtagga tttagcgtgt
    24961 tgatcagatc aattgccaca ctccgccgat attcagcaat atttgccacc gcaaaagcag
    25021 tatcaataac ggactgtttc aggccataca cccgttcctt taccatttga gtgtagatcc
    25081 cgcaggcaac tgctgaaatc atgataaaaa tcagcagact gactgtaagt acgaatttat
    25141 tgattctgga catacgtaaa ctactctttt actaattttc cacttttatc ccaggcggag
    25201 aatggcatgg gtttctggta cagatacccc tgagcctgaa cacatcctaa tttgattaat
    25261 tcacgcgcaa cgtcttcgct ctctacgcct tccgcaatca ccgggatccg gttatagcgt
    25321 gccagatcga aaataatgcg cacaattttt cccgcagtac cgaaaatatc gccggtaaat
    25381 tgcgaatcca cctttatact gtctggatta atttcataaa aacgtttcag gtttgacaaa
    25441 ccggtgccaa agtcatcaat cgatacccgt accccttgtt tacgcagttg ttcaatattg
    25501 agatagaaat gttcattaat tcgctcgatc acggtttcgg tgatctcaac acaaaggctg
    25561 ttcgccttca ggccattttc tttcatgatg gtagtaaaac gctcatgaaa gtgtggttcg
    25621 tttaactgcc agggcgaaac attaatatgc agtaagaaat catccttcag cgacgcacgc
    25681 tgactaaaat gtctgaattc acccatcgcg gttttaataa tttgatagcc cagctcattg
    25741 ataaacccga tatcttcagc aatggtaatg aacttcagcg gtgaaataat cccccgctcc
    25801 gcagacaccc aacggcacaa cgcctccgcg cccaaaatgg tgcgggtgtt aatatcaaca
    25861 atcggttgat agaccaggaa tagttcgcca atttccattg catggcgaat attacgcgca
    25921 atccagatat tctcttctat ttcgttgagt ttcgcatcat cacagaaaat aaactctgag
    25981 aacatatggt tgcggatact ggaaatcgcc agtttcagct tttctgcgta actccaggcg
    26041 ttttccccct gcaaaggttc acaaatagca gacttatgga tgcgcagatt gcggtagcca
    26101 aaactgctgt caaaatcgtt taccgtcgct atcttacggg taaacgtctg tacattttcc
    26161 cggcacagga gtaaatagag atcggcatta aatgtcacga tatcgcagca ttgcgcatat
    26221 ttctcggcaa ttcgctgaca caatttcctg acgattttat tgcgttcatc aacaccaaac
    26281 accgcttcca cagcttccag accacacagt ttgatgatcc ccacagccag atactgatta
    26341 ttgttggact caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc
    26401 agcgagagca atttatttgc ccgttcaatt tcagcatctt tcatttccgc gatcgaattt
    26461 ttgatgctga taatctcttt cgcatcgcct tcacgaatct tcagggcctc gatttgatcc
    26521 gtagagtcag gcaaaaacgc cagcgtattg accaaatctg tcatttcaga aaattgacga
    26581 cggaaaactc gagtggtcat taaataaacc accagcaata cgataataat gaaagcaaac
    26641 ggcagataaa aaagtttatc tgatgcgttc aataaattac caccaggata ataaaaatag
    26701 atttcgaata atgcgttgtt tggcaccgct gatgtttttt caatatattc cgtatcgaca
    26761 gaatcaataa tattataatt accttcgcta ttataaataa tcaatttttc acgagcgagt
    26821 tgattcttag caatgatctc atgaccttgc tttaaaaata gaacttcatc attagcgctg
    26881 atgtgatcaa gatactcgtt cagatatcgc cagtttaaat cgacatgaat tttatctacc
    26941 gctaacaaat catgatcttt gccgttgatg tgtagcgtaa aagagataca ttttttaccc
    27001 ggacgatcaa agcaattgta tgccgggttc cagctgttgt tagagtcatc tgaagccatc
    27061 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
    27121 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
    27181 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
    27241 tccattttca gaatcacggc acgttgaatc tcctctgtat tttttaataa atacagtgag
    27301 tcctttaatg tcaggg
//
LOCUS       DAPQZD010000053        27006 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPQZD010000053 DAPQZD010000000
VERSION     DAPQZD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.053"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            102..1571
                     /gene="gspA"
                     /locus_tag="RO454_002192"
     CDS             102..1571
                     /gene="gspA"
                     /locus_tag="RO454_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDY0934023.1"
                     /translation="MSTRREVILSWLREKHQTWRLCYLLGEAGSGKTRLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTCVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1573..1992
                     /gene="gspB"
                     /locus_tag="RO454_002193"
     CDS             1573..1992
                     /gene="gspB"
                     /locus_tag="RO454_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDY0934024.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2230..2541
                     /gene="rpsJ"
                     /locus_tag="RO454_002194"
     CDS             2230..2541
                     /gene="rpsJ"
                     /locus_tag="RO454_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDY0934025.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2574..3203
                     /gene="rplC"
                     /locus_tag="RO454_002195"
     CDS             2574..3203
                     /gene="rplC"
                     /locus_tag="RO454_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDY0934026.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3214..3819
                     /gene="rplD"
                     /locus_tag="RO454_002196"
     CDS             3214..3819
                     /gene="rplD"
                     /locus_tag="RO454_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDY0934027.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3816..4118
                     /gene="rplW"
                     /locus_tag="RO454_002197"
     CDS             3816..4118
                     /gene="rplW"
                     /locus_tag="RO454_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDY0934028.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4136..4957
                     /gene="rplB"
                     /locus_tag="RO454_002198"
     CDS             4136..4957
                     /gene="rplB"
                     /locus_tag="RO454_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDY0934029.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4974..5252
                     /gene="rpsS"
                     /locus_tag="RO454_002199"
     CDS             4974..5252
                     /gene="rpsS"
                     /locus_tag="RO454_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDY0934030.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5267..5599
                     /gene="rplV"
                     /locus_tag="RO454_002200"
     CDS             5267..5599
                     /gene="rplV"
                     /locus_tag="RO454_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDY0934031.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5617..6318
                     /gene="rpsC"
                     /locus_tag="RO454_002201"
     CDS             5617..6318
                     /gene="rpsC"
                     /locus_tag="RO454_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDY0934032.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6331..6741
                     /gene="rplP"
                     /locus_tag="RO454_002202"
     CDS             6331..6741
                     /gene="rplP"
                     /locus_tag="RO454_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDY0934033.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6741..6932
                     /gene="rpmC"
                     /locus_tag="RO454_002203"
     CDS             6741..6932
                     /gene="rpmC"
                     /locus_tag="RO454_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDY0934034.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6932..7186
                     /gene="rpsQ"
                     /locus_tag="RO454_002204"
     CDS             6932..7186
                     /gene="rpsQ"
                     /locus_tag="RO454_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDY0934035.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7351..7722
                     /gene="rplN"
                     /locus_tag="RO454_002205"
     CDS             7351..7722
                     /gene="rplN"
                     /locus_tag="RO454_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDY0934036.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7733..8047
                     /gene="rplX"
                     /locus_tag="RO454_002206"
     CDS             7733..8047
                     /gene="rplX"
                     /locus_tag="RO454_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDY0934037.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8062..8601
                     /gene="rplE"
                     /locus_tag="RO454_002207"
     CDS             8062..8601
                     /gene="rplE"
                     /locus_tag="RO454_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDY0934038.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8616..8921
                     /gene="rpsN"
                     /locus_tag="RO454_002208"
     CDS             8616..8921
                     /gene="rpsN"
                     /locus_tag="RO454_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDY0934039.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8955..9347
                     /gene="rpsH"
                     /locus_tag="RO454_002209"
     CDS             8955..9347
                     /gene="rpsH"
                     /locus_tag="RO454_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDY0934040.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9360..9893
                     /gene="rplF"
                     /locus_tag="RO454_002210"
     CDS             9360..9893
                     /gene="rplF"
                     /locus_tag="RO454_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDY0934041.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9903..10256
                     /gene="rplR"
                     /locus_tag="RO454_002211"
     CDS             9903..10256
                     /gene="rplR"
                     /locus_tag="RO454_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDY0934042.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10271..10774
                     /gene="rpsE"
                     /locus_tag="RO454_002212"
     CDS             10271..10774
                     /gene="rpsE"
                     /locus_tag="RO454_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDY0934043.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10778..10957
                     /gene="rpmD"
                     /locus_tag="RO454_002213"
     CDS             10778..10957
                     /gene="rpmD"
                     /locus_tag="RO454_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDY0934044.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10961..11395
                     /gene="rplO"
                     /locus_tag="RO454_002214"
     CDS             10961..11395
                     /gene="rplO"
                     /locus_tag="RO454_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDY0934045.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11403..12734
                     /gene="secY"
                     /locus_tag="RO454_002215"
     CDS             11403..12734
                     /gene="secY"
                     /locus_tag="RO454_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDY0934046.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12766..12882
                     /gene="rpmJ"
                     /locus_tag="RO454_002216"
     CDS             12766..12882
                     /gene="rpmJ"
                     /locus_tag="RO454_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDY0934047.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13029..13385
                     /gene="rpsM"
                     /locus_tag="RO454_002217"
     CDS             13029..13385
                     /gene="rpsM"
                     /locus_tag="RO454_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDY0934048.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13402..13791
                     /gene="rpsK"
                     /locus_tag="RO454_002218"
     CDS             13402..13791
                     /gene="rpsK"
                     /locus_tag="RO454_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDY0934049.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13825..14445
                     /gene="rpsD"
                     /locus_tag="RO454_002219"
     CDS             13825..14445
                     /gene="rpsD"
                     /locus_tag="RO454_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDY0934050.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14471..15460
                     /gene="rpoA"
                     /locus_tag="RO454_002220"
     CDS             14471..15460
                     /gene="rpoA"
                     /locus_tag="RO454_002220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDY0934051.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15501..15884
                     /gene="rplQ"
                     /locus_tag="RO454_002221"
     CDS             15501..15884
                     /gene="rplQ"
                     /locus_tag="RO454_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDY0934052.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15991..16359
                     /locus_tag="RO454_002222"
     CDS             15991..16359
                     /locus_tag="RO454_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDY0934053.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16370..16795
                     /gene="zntR"
                     /locus_tag="RO454_002223"
     CDS             16370..16795
                     /gene="zntR"
                     /locus_tag="RO454_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDY0934054.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16851..17069
                     /gene="arfA"
                     /locus_tag="RO454_002224"
     CDS             16851..17069
                     /gene="arfA"
                     /locus_tag="RO454_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDY0934055.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17066..17479)
                     /gene="mscL"
                     /locus_tag="RO454_002225"
     CDS             complement(17066..17479)
                     /gene="mscL"
                     /locus_tag="RO454_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDY0934056.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17609..18985)
                     /gene="trkA"
                     /locus_tag="RO454_002226"
     CDS             complement(17609..18985)
                     /gene="trkA"
                     /locus_tag="RO454_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDY0934057.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19007..20296)
                     /gene="rsmB"
                     /locus_tag="RO454_002227"
     CDS             complement(19007..20296)
                     /gene="rsmB"
                     /locus_tag="RO454_002227"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDY0934058.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20348..21295)
                     /gene="fmt"
                     /locus_tag="RO454_002228"
     CDS             complement(20348..21295)
                     /gene="fmt"
                     /locus_tag="RO454_002228"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDY0934059.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21310..21819)
                     /gene="def"
                     /locus_tag="RO454_002229"
     CDS             complement(21310..21819)
                     /gene="def"
                     /locus_tag="RO454_002229"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDY0934060.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21949..23073
                     /gene="dprA"
                     /locus_tag="RO454_002230"
     CDS             21949..23073
                     /gene="dprA"
                     /locus_tag="RO454_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDY0934061.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23045..23518
                     /locus_tag="RO454_002231"
     CDS             23045..23518
                     /locus_tag="RO454_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDY0934062.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23547..24089
                     /locus_tag="RO454_002232"
     CDS             23547..24089
                     /locus_tag="RO454_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDY0934063.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            24094..24666
                     /gene="tsaC"
                     /locus_tag="RO454_002233"
     CDS             24094..24666
                     /gene="tsaC"
                     /locus_tag="RO454_002233"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDY0934064.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24671..25489
                     /gene="aroE"
                     /locus_tag="RO454_002234"
     CDS             24671..25489
                     /gene="aroE"
                     /locus_tag="RO454_002234"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDY0934065.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25486..25743
                     /locus_tag="RO454_002235"
     CDS             25486..25743
                     /locus_tag="RO454_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDY0934066.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25719..26273)
                     /locus_tag="RO454_002236"
     CDS             complement(25719..26273)
                     /locus_tag="RO454_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDY0934067.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
     gene            26744..>27006
                     /locus_tag="RO454_002237"
     rRNA            26744..>27006
                     /locus_tag="RO454_002237"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6928 a         6333 c         7038 g         6707 t
ORIGIN      
        1 atcaagcaaa atttatttat attagtaata tgtatttaat taataaatac atacattatt
       61 tttatgatta aggataatca ataattaaaa caggaagttc tatgtctacg agaagagaag
      121 tcattctttc ctggttgcgt gagaagcatc agacctggcg tctgtgctat ttgttgggtg
      181 aggctggaag tggaaaaacc cggctggcgc agcaactaca aaaagataaa catcgccgtg
      241 tgattacttt aagcctcgtt gtttcctggc aaggtaaggc cgcatggatc gttaccgacg
      301 ataatgcggc tgaacagggc tgccgtgaca gcgcctggac gcgagatgag atggcgggtc
      361 aattactgca tgcacttcat cgaactgata gtcgctgccc tcttattatt attgaaaatg
      421 ctcacctgaa tcaccgccgc atacttgatg atctccaacg cgcgatatct cttattcctg
      481 acggtcaatt tttgctgatc ggcagacccg atcgaaaagt cgaacgtgat tttaaaaaac
      541 agggcattga acttgtctcg acaggtcgtc tgacggagca cgagcttaag gcaagcatcc
      601 ttgaaggtca gaatattgac cagcccgatc tccttttaac cgccagagtt ctgaaacgga
      661 tagctttatt atgtcgggga gatcgcagaa agctggcgct tgccggcgaa acaattagct
      721 tattacagca agccgagcag acctgcgttt tcactgcaaa acaatggcga atgatttacc
      781 gcgtattagg tgataaccgc ccccggaaaa tgcagcttgc cgtggtgatg agtggaacca
      841 ttatcgcact tacctgtggt tggctactgc tgccctcttt cacagctccg ttacccgttc
      901 ctgcgtggct gatacccgta acgccagtag ttaaacaaga tatgactaag gatattgccc
      961 atgtggtgat gcgcgatagt gaggcgctaa gcgtgttata cggcgtatgg gggtacgagg
     1021 tcccagccga ttcggcctgg tgcgatcagg cagtaagagc ggggctggta tgtaaaagtg
     1081 ggaatgcttc tttacaaaca ttggtagatc agaatttgcc ctggatcgca tcgcttaaag
     1141 tcggggacaa aaaattgcct gttgtggtcg ttcgcgtcgg tgatgctacc gttgatgtac
     1201 tcgtagggca gcaaacctgg acgctgacac ataaatggtt tgagttggta tggacgggtg
     1261 attatcttct gttgtggaaa atgtcgccgg aaggagagag taccattacg cgtgatagca
     1321 gcgaggaaga gattctctgg ctggaaacga tgttaaaccg cgcattgcat atttcgacag
     1381 aatcttcggc agagtggcgt cccttgttgg tagaaaaaat aaagcaattt caaaaaagcc
     1441 atcacttgaa aactgacggt gttgttggct tttcaacgtt agtgcatctc tggcaggtgg
     1501 caggggagag tgcctactta tatcgggatg aagcaaatat ttccccggaa acaacagtga
     1561 aggggaaatg acatgtttga attctatatc gccgcgcgtg agcagaaaga aacaggtcac
     1621 cctggtatct tctcccgaca aaaacacagc accataatat atgtcatttg tttattgctg
     1681 atttgcctgt ggtttgcagg aatggtgttg gttggtgggt atgcccggca gctatgggca
     1741 ctttggatag taaaagcaga agtcactgta gatgctgaaa cgcccgcttt taaacaatca
     1801 acacaacact attttttcaa aaaacagcca ctccccgtcg tcgaatccgt cgaggaggaa
     1861 gatgacccag gagtcgcggt tgagaatgcc ccctcttctt ctgaagacga agacaatact
     1921 gtagaagaaa gcgacgagaa agccggttta agagagcgtg tcaaaaatgc gctgaacgaa
     1981 cttgagagat aacccaaagg ctgtttactt tactagcaat acgcttgcgt tcggtggtta
     2041 agtatgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga acctcagtta
     2101 gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa cggttacact
     2161 ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa taattggagc
     2221 tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga tcatcgtctg
     2281 atcgatcaag caaccgcgga aatcgtcgag actgctaagc gcactggtgc gcaggtccgt
     2341 ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc cccgcacgtc
     2401 aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt
     2461 gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc cggtgtagac
     2521 gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa acaatgattg
     2581 gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc gtttctatcc
     2641 cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac ctggctaacg
     2701 atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc
     2761 ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg tgggaattcc
     2821 gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg
     2881 acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca ggtaccgtta
     2941 agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct caccgcgttc
     3001 cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag aaaatggcag
     3061 gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc gttgacgctg
     3121 agcgcaacct gctgctggtt aaaggcgctg tcccgggtgc aaccggtagc gacctgatcg
     3181 ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa agacgcgcag
     3241 agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc gctggttcac
     3301 caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca gaagactcgt
     3361 gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt
     3421 tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc tgctcgtccg
     3481 caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg
     3541 tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt agaagcgccg
     3601 aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt gctgatcatc
     3661 accggtgagc tggacgagaa cctgttcctg gctgcgcgca acctgcacaa ggttgacgta
     3721 cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt cgtaatgact
     3781 gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga
     3841 aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca
     3901 ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga
     3961 aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg aaagttaaac
     4021 gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc accctgaaag
     4081 aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta atacaatggc
     4141 agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag tggttaaccc
     4201 tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca aatccggtgg
     4261 tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca agcaggctta
     4321 ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg aacgtcttga
     4381 gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg gtgaacgccg
     4441 ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg gcgttgatgc
     4501 tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt ctactgttca
     4561 taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg gtacttacgt
     4621 tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg
     4681 taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg agcatatgct
     4741 gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg
     4801 taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta actttggtaa
     4861 gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca gcaacaagcg
     4921 tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa gccatgccac
     4981 gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta gagaaagcgg
     5041 tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg atctttccta
     5101 acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg gtatttgtaa
     5161 ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact tatcgcggcc
     5221 acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg aaactatcgc
     5281 taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg acctgattcg
     5341 cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga aagcggctgt
     5401 actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg atggcgctga
     5461 cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat
     5521 tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc acatcactgt
     5581 ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt acatcctaat
     5641 ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa caccaaagaa
     5701 ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa ggaactggct
     5761 aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg tgtaaccatt
     5821 cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga aaaactgcgt
     5881 aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga agttcgtaag
     5941 cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga acgtcgcgtt
     6001 atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt
     6061 attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac
     6121 cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa cacctctgaa
     6181 gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg
     6241 ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa aaagcagcag
     6301 cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac aaaattccgt
     6361 aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag cttcggcagc
     6421 ttcggtctga aagctgttgg ccgtggtcgt ctgactgcac gtcagatcga agcagcacgt
     6481 cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt gttcccggac
     6541 aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg taacgtggag
     6601 tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa
     6661 gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa aaccaccttt
     6721 gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc gttgaagagc
     6781 tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa
     6841 gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta
     6901 agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg
     6961 tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac gttttgtgaa
     7021 acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa
     7081 caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc tgtccaagac
     7141 taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca gtacactctc
     7201 tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat atccttgaag
     7261 cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc
     7321 agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat gctgaacgtc
     7381 gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt
     7441 cgctacgcag gcgtaggcga tatcatcaag atcaccatca aagaagcaat tccgcgtggt
     7501 aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt
     7561 cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac
     7621 agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag
     7681 aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa tcatggcagc
     7741 gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag gtaaacgcgg
     7801 taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca atctggttaa
     7861 gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc
     7921 cgctattcag gtttccaacg tagcaatctt caatgcgaca accggcaagg ctgaccgtgt
     7981 aggctttaga ttcgaagacg gtaaaaaagt ccgtttcttc aagtctaaca gcgaaactat
     8041 caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt
     8101 aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg ggtcgagaag
     8161 atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct ggataacgca
     8221 gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct
     8281 gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac tctgcgtggc
     8341 gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg tatccgtgac
     8401 ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat gggtgtccgt
     8461 gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg tggtttggat
     8521 attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct ggctgccttt
     8581 gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca atgaaagcac
     8641 gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct gaactgaaag
     8701 cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc
     8761 agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc
     8821 gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt gaagccgcta
     8881 tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca attgaatcac
     8941 gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc cgtatccgta
     9001 acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg aaagtggcaa
     9061 tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa ggcgacacca
     9121 agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta gaaagcattc
     9181 agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta
     9241 tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact gatcgtgcag
     9301 cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg gaggaaaaaa
     9361 tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta aaaatcaacg
     9421 gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc aacgatgctg
     9481 ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt tacgcagacg
     9541 gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc ggtgttaccg
     9601 aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg gttaaaggca
     9661 atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg cctgcgggta
     9721 tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat aagcaggtga
     9781 tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttac aaaggcaagg
     9841 gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag taaggtaaca
     9901 ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc aagctccagg
     9961 agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac gcacaggtaa
    10021 ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa gctatcgctg
    10081 aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa gctgtcgctg
    10141 aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg ttccaatatc
    10201 atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag ttctaaggta
    10261 gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa agctgatcgc
    10321 ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca cagctctgac
    10381 tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc gtgaagttcc
    10441 agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg tcgcgctgaa
    10501 taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg tattcatgca
    10561 gccggcttcc gagggtaccg gtatcatcgc cggtggtgca atgcgcgccg ttctggaagt
    10621 cgctggggtt cataacgttc tggctaaagc gtatggttcc accaacccga ttaacgtggt
    10681 tcgtgcaact atcgatggcc tggaaaatat gaattctcca gaaatggtcg ctgccaagcg
    10741 tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta ttaaaattac
    10801 tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc ttggcctggg
    10861 tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc gcggtatgat
    10921 caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa atactctgtc
    10981 tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg gttctggcct
    11041 cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg gtggcgtacg
    11101 tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat tcggcttcac
    11161 ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta aagtagaagg
    11221 cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc agatcgagtt
    11281 cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg gcctgcgtgt
    11341 tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg aataagtagc
    11401 agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta ggcgagctga
    11461 aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc tcttttattc
    11521 cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag cgaggcacca
    11581 tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct atctttgctc
    11641 tggggatcat gccgtatatt tcggcgtcga tcattatcca gctactgacg gtggttcatc
    11701 caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc agccagtaca
    11761 cccgctacgg tactctggtg ctggcaatat tccagtcgat cggtattgct actggtctgc
    11821 cgaatatgcc tggtatgcaa ggcctggtga ttaacccggg ctttgcattc tacttcaccg
    11881 ctgttgtaag tctggtcaca ggaaccatgt tcctgatgtg gttgggcgaa cagatcactg
    11941 aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc gcgggactcc
    12001 cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac ttcctcgtgt
    12061 tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt gttgagcgtg
    12121 gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt gtctatgctg
    12181 cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg gcaatcttcg
    12241 cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc ggtactggtt
    12301 ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt tatgtgttac
    12361 tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt ttcaacccgc
    12421 gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt cgtccgggag
    12481 agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccttggtt ggtgcgctgt
    12541 acattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa gtaccgttct
    12601 acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt atggctcaag
    12661 tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg aacctgaaag
    12721 gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa agttcgtgct
    12781 tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt catccgtgtg
    12841 atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg catatttttc
    12901 ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg tctgtgcgtt
    12961 tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt aaatagtagg
    13021 agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat gccgtaatcg
    13081 cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg gctgcagcgg
    13141 gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac acgctgcgtg
    13201 acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc atgagcatca
    13261 agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt ctcccggttc
    13321 gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa ccgatcaaga
    13381 aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa acgtgtaaga
    13441 aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac catcgtgact
    13501 atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc cggtttccgt
    13561 ggttctcgca aatccactcc gtttgcagca caggttgcag cagagcgttg cgctgacgtc
    13621 gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg tccaggccgc
    13681 gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat tactgatgtg
    13741 actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata acgcctcgtt
    13801 ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct caagctgagc
    13861 cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga taccaagtgt
    13921 aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc tgactatggt
    13981 gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga gcgtcagttc
    14041 cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa cctgttggct
    14101 ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc cactcgtgca
    14161 gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt tgttaacatc
    14221 gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc gaagaagcag
    14281 tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac ctggctggaa
    14341 gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc tgatctgtct
    14401 gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt agtaccaaag
    14461 agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga
    14521 gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg gctttggcca
    14581 tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt gcgcggtgac
    14641 cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg ttcaggaaga
    14701 tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg gcaaagatga
    14761 agttattctt accttgaata aatctggcat tggccctgtg actgcagccg atatcaccca
    14821 cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga ccgatgagaa
    14881 cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac
    14941 ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg acgcatgcta
    15001 cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga
    15061 cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg aagaggcgat
    15121 tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact tacgtgatgt
    15181 acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc tgcgccctgt
    15241 tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag ctatccacta
    15301 tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta accttggtaa
    15361 aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc tgggcatgcg
    15421 cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca ggttaaggtt
    15481 ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg aaccgcaaca
    15541 gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt catgaaatca
    15601 tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg ctgattactc
    15661 ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact cgtgataacg
    15721 agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt gccggtggtt
    15781 acactcgtat tctgaagtgt ggcttccgtg caggcgacaa tgcgccgatg gcttacatcg
    15841 agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa gcaacgtaaa
    15901 aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct acaatagctg
    15961 tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg ggcagagcgt
    16021 catatagcag aagcgcaagc gaaaggtgag tttgataacc tggtaggtag cggcgaacca
    16081 ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta tcgcttgctg
    16141 aagaatgctg gttgcttacc gccagaactt gagcatagga gggaagcaat acagcttctg
    16201 gatattctca aaggtatccg tcatgatgat ccgcaatatc aagaggttag tcgtcgattg
    16261 tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt acgcggcgat
    16321 tatgctgaca agttgttgaa caaaatcaac gataactagt ggagtatgta tgtatcgcat
    16381 tggtgagctg gcaaaaatgg cggaagtaac acccgacacg attcgttatt acgaaaagca
    16441 gcagatgatg gagcatgaag tacgcaccga aggtgggttt cgcctgtata ccgaaagcga
    16501 tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc tggagtcgat
    16561 ccgcgagttg ctgtcgatcc gtatcgatcc tgaacaccat acctgtcagg agtcaaaagg
    16621 cattgtgcag gaaagattgc aggaagtcga agctcgtata gccgagttgc agagtatgca
    16681 gcgttccttg caacgcctta atgatacctg ttgtgggacc gcccatagca gtgtttattg
    16741 ctcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct gttgattttt
    16801 tgcactggca ggattacact cgcgccgtta aataaccaat tggagttttt atgagccgat
    16861 atcagcatac taaagggcag ataaaggata atgcgataga agcattacta catgatccct
    16921 tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg cgaaaaggaa
    16981 aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac ttttttacca
    17041 ctggccttct gctttcagac gcttgttaag agcggttatt ctgctctttc agcaaatcac
    17101 gaatttctgt cagtaatact tcttctttag ttggtgcagg tgttgctgct gctggttctt
    17161 cttttttccg attcagtttg ttgattagct taatcgccat aaagatggca aaggccacta
    17221 tcagaaaatc aaagacgttt tgaatgaaga caccgtaatg catcacaaca gcagggatat
    17281 ccccttgggc ctcgcgtagc gtgacagcaa actgtttaaa gtcgatcccg ccaattaata
    17341 agcccagagg tggcatgatg atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg
    17401 ctccgataat gacacccact gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa
    17461 attctttaat aatgctcatg ttattctccc tatgaagccg acaaccataa gtctaacaaa
    17521 tgttaagcca ttttcctgcc aggaatggat ttaattaatc cttaataatc aaagggaaat
    17581 aataaggcgt cattagacgc cttattaatt acaagaagaa aggacttggc tggaagagtc
    17641 tttcgacgtc ggtaataaac tttttatctg tgaggaacat aattacgtga tcgccttgct
    17701 caatgcgcag attgtcattg gcaatcatca catcgtttcc acgtaccacc gctccaataa
    17761 tcgttcctgg cggtagcttg atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt
    17821 catcaccgtg agcaacggct tcaatagctt ctgctacgcc gcggcgtaat gaggaaacac
    17881 caacaatatc tgcttttcgc acatggctaa gcaacgcaga aatagttgct tgttgtggtg
    17941 aaatcgcaat atcgatcacg ctcccctgaa ccagatccac ataagcgcga cgttggatca
    18001 ataccatcac ctttttcgca cccatacgtt tggcaagcat ggcggacatg atattggcct
    18061 cgtcatcgtt ggtgacggca ataaacagat caacttgatc gatatgttct tcggccagca
    18121 gttcttgatc cgacgcatca ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt
    18181 cggcagcgcg ctgctgatta cgttcgatga gtttaacgct gtaatctttt tccagacgac
    18241 gcgccagccc tgcaccgata ttaccgccac caaccagcat gatccgctta tacggttttt
    18301 ccagtcgctg taattcactc atcaccgcac ggatatgctg tgaagcggca ataaagaaca
    18361 cttcatcacc agcttcaaca atggtcgaac cttgcggacg aatggggcga tcgtggcgga
    18421 aaattgctgc cacacgagta tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat
    18481 taccaatcag cgggccgcca taataggctt taaccacagc caggctgact ttaccctcag
    18541 cgaagttcac cacctgcaat gcgccgggat actcaatcag tcggtaaata ttatcgataa
    18601 ccaactgctc tggtgcgatc agatgatcaa tcggtacagc atctgaatga aatagtttat
    18661 cggcatcgcg cacgtagtct ggtgagcgga tacgagcgat gcgattaggg gtgttgaaaa
    18721 gtgagtaggc tacctggcag gcaaccatat tggtttcatc tgaactggtt acagcaacca
    18781 gcatatcggc gtcgtcggca cctgcctccc gcaatacgcg tggatgagag ccatgcccct
    18841 gcacgacccg caggtcaaat ttatcctgta aggtccgcag acgctcaccg ttggtatcga
    18901 caacagtaat atcgttgttc tcgccaacca ggttttccgc cagtgtgccg ccaacctggc
    18961 cggcacccag aatgataatt ttcatcagtc gcgacccgtt atctcatcac tttttgatta
    19021 gcttagcgta aaagaagccg tcgccctctt cggcaccggg caaattttgt ttacccggtt
    19081 gctctggtgt tcctgtttca caaagttcgg catcagcggt acgttgcaaa aaggctttaa
    19141 tctgcaggct attttcttcc ggtaacaccg aacaggtggc atagaccagg gttccaccgg
    19201 attttaaatg tggccaaatg gcatcgagta tttcagactg caattgcgcg agttccggga
    19261 tatcgcgatc acgacgtaac catttaatat ccggatggcg acgaatcacc cccgttgctg
    19321 aacaaggcgc atctaataaa atgcgatcaa actgttgctc gccacaccat tgggaagggt
    19381 tgcggccatc accttgtttc acggtcgcct tcataccaag gcgttttaaa ttgtcgtaaa
    19441 cgcgagagag gcgctgctca tcaatatcga ccgccagaac ttgcgcctct ggagcaacct
    19501 caagaatatg cgttgtttta ccgcctgggg cggcgcaaag atccagaatg tgttcaccat
    19561 tttgtggagc aagccaggtc atgcaacctt gtgctgaagc atcctgaacg gtaacccatc
    19621 cttcttcaaa accgggtagc gcatgaacag gtgcaggtgt ttccagctgt acagcatcag
    19681 ggtaatccgc atgcgggaaa cctttcattc ctgcttcatc cagcaatgca agccagctgt
    19741 cgcgggaatg atgcgtacga ttaacgcgca gccacattgg cggacgctgg ttattggctt
    19801 cgacgatgga ttgccactgc tcgggatacg ccttttgcag acgcttcagc aaccaggaag
    19861 gatgcagata acgtgcatca ctggcattaa actcggctaa taactcatct tgctgacgct
    19921 ggaactgacg taatacaccg ttaatcaatc ccttaagttg cgggcgctta attgcaactg
    19981 cgccttcaac cgtttcagcc agcgcggcat gaggtggaat gcgggtataa agcagttgat
    20041 acaaaccaac cataatcagg taatgaacag tacgctgttt gcctgtcatc ggacgggcca
    20101 ttagcttatt aatcagccag tctaactgtg agagcgtacg cagtacgcca aagcataact
    20161 cctggagaag agctttatct ttatcggaaa ctttttgctg gagcggtggc agaatgttgc
    20221 tcagtgattg cccttgctca acgacttgtt caatggcctg ggccgccatg ctacgtaaat
    20281 tacgttgttt tttcataagt ataaatataa agatgcccgg taagaccggg cttagaagag
    20341 tggactatca ggccagacgg ttgcccggta caaaccattc ccgacgagaa ttcaggaggt
    20401 cttgcacgct catcgctttc ttacctgcag gttgcattga gagcagattc aggatgccat
    20461 cacctgtcgc tacctgaatg ccttgtttat tggcttcaag gattgttcct ggcgcagcct
    20521 ttgttgtcgt atcgatgaca gatgctttcc agactttaac cggctgccct tcaatttcca
    20581 gccagctcat tggccatgga ttgaaagcgc gaatgcaacg ttcaagctgt gctgccgaaa
    20641 gtgaccagtc aatacgcgct tcttctttac tcaacttttc ggcgtaagtg acaagagttt
    20701 cgtcctgaac ttctggtttc gccgtgccgt ctgccagttg tttcaacgtg gtgataagcc
    20761 cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa
    20821 tcgggcagga gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa
    20881 tggtcacacc agtttctgca tcacccgccc atagtgagcg ttggattggt gctgcaccgc
    20941 gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca
    21001 cagctttagg cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcagatcgg
    21061 cgaccagttg ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt
    21121 tgtcctcagc cagaacttta accgggctgg gcatcagttt tttaccgcgt cctgccggtc
    21181 ggtctggctg ggtgaacacg ccaacgatgt tatgaccaga agacaacagc gcgtcgagat
    21241 gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttaa
    21301 ttcttatcct taagcccggg ctttcagacg atccagtttt tcaactttct gacgaatacg
    21361 ttgttgtttc agcggtgaca gataatccat aaacagtttg ccgaccaggt gatccatctc
    21421 atgctgaata cagatggcta acagaccgtc tgcttccagt tcaaatggtt taccgtcgcg
    21481 gtccagggcg cgaattttaa ctttctctgc gcgtggcact aaagcacgtt gttcagggat
    21541 cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat
    21601 taacaccagc cgttcgtcac ggttttccga aacatcaatt acaatgatac gttgatggat
    21661 atcaacctgg gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc
    21721 atcgacgata cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag
    21781 ccgctcgtcc ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca
    21841 ggagttagaa agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac
    21901 tgaccaatcg caaagattgc taaggctgct tatggcaggg agataaggat ggtcgataca
    21961 gaaatttggc tgcgtttaat gagcatcagc agcttgtatg gcgatgatat ggtccgtata
    22021 gctcactggc tggcaaaaca gtcgcatatt gatgcggttg tattgcagca aacagggctt
    22081 acattgcggc aggcacaacg ctttctttca tttccgcgaa agagtatcga aagctcactt
    22141 tgttggttgg agcaacccaa ccatcattta atccctgcgg acagcgaatt ttatcctcct
    22201 caacttctgg cgacgacaga ttaccccggc gcactgtttg ttgaaggaga actgcacgcg
    22261 ttgcattcat ttcagcttgc cgtagtgggg agtcgggcgc attcatggta tggcgagcga
    22321 tggggacgat tattttgcga aactctggcg acgcgtggag tgacaattac gagtggactg
    22381 gcgcgtggaa tcgatggtgt ggcgcataaa gcggccttac aggtaaatgg cgttagcatt
    22441 gctgtattgg ggaatggact taataccatt catccccgcc gccatgctcc actggctgcc
    22501 agtctacttg agcaaggtgg tgctctcgtc tcggaatttc ccctcgatgt tccacccctt
    22561 gcttacaatt tcccacgaag aaatcgcatt atcagtggtc taagtaaggg tgtactggtg
    22621 gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac gttgtgcgct tgagcagggg
    22681 cgtgaagttt ttgctttgcc aggtccaata gggaatccgg gaagcgaagg gcctcactgg
    22741 ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag aaattctgga aaacttgcaa
    22801 tttggattgc actggttgcc agacgcccct gaaaattcat tttattcacc agatcaggaa
    22861 gacgtggcat tgccatttcc tgagctcctg gctaacgtag gagatgaggt aacacctgtt
    22921 gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg tagttactca actactcgaa
    22981 ctggagttag caggatggat cgcagctgta cccggcggct atgtccgatt gaggagggca
    23041 tgccatgttc gacgtactaa tgtatttgtt tgaaacctat attcacacag aagctgagtt
    23101 gcgtgtggat caagacaaac ttgaacagga tcttaccgac gcaggatttg atcgagaaga
    23161 tatctacaat gccctgcttt ggctggaaaa gcttgcggat tatcaggaag gactggcaga
    23221 accgatgcaa ctggcctctg atccactctc catgcgtatt tatacccctg aagagtgtga
    23281 aagactggat gccagctgtc gtggtttttt gcttttcctt gagcaaattc aggtgcttag
    23341 ccttgaaacc cgtgaaatgg tgatagagcg agtgctcgcg ctggataccg cagagttcga
    23401 cctggaagac ctgaaatggg taatcctgat ggtgttgttc aatattccgg gttgcgaaaa
    23461 tgcgtaccag caaatggaag aattactctt tgaagtgaat gaaggtatgc tgcattaatc
    23521 atttctttaa ttcagcataa gttgttatgg cgaaatcagc actgttcacg gtgcgtaata
    23581 atgagtcctg cccaaagtgc ggggctgaac tagttattcg atccgggaaa cacggtccgt
    23641 ttcttggatg ctcacagtat ccggcatgtg gctacgtccg tcctctgaaa tcttcagcgg
    23701 atggacatat tgtcaaagtt ctggaggggc aggtttgccc tgcatgtggc gcaaatctgg
    23761 tattacgcca gggacgcttt ggtatgttta ttggttgcag taactaccct gaatgcgaac
    23821 ataccgaact tatcgataaa ccggacgaaa cagcaattac atgcccccaa tgccggacgg
    23881 gccatctggt tcagcgccgc tcccgttatg gcaaaacatt tcactcctgt gatcgctacc
    23941 cggagtgtca atttgccatt aacttcaaac ccatagctgg agaatgccct gagtgccatt
    24001 atccgctact catcgaaaag aaaaccgcgc agggtgtaaa acacttttgt gccagtaaac
    24061 aatgtggaaa gccgatttcg gcggaataat aacgtgaata ataacctgca aggagacgct
    24121 atcgcagctg cgatagatgt cctcaatgaa gaacgtgtca tcgcctatcc aacggaagcc
    24181 gttttcggtg tcgggtgcga tcctgatagc gaaacagcag tgatgagact gttggagtta
    24241 aaacggcgtc cggttgataa ggggctgatt ttaatcgcgg caaattacga gcagcttaaa
    24301 ccctatattg atgacaccat gctgactgac gcgcagcgtg aaaccatttt ttcccgctgg
    24361 ccaggtcctg taacctttgt ctttcccgcg cctgcgacaa caccgcgctg gttgacgggc
    24421 cgctttgatt cgcttgctgt acgagtcacc gaccatccgt tggtggttgc tttgtgccag
    24481 gcttatggta aaccgctggt ttctaccagt gccaacttga gtggattgcc accttgtcga
    24541 acagtagacg aagttcgcgc acaatttggc gctgcgttcc cggttgtgcc tggtgaaacg
    24601 ggggggcgtt taaatccttc agaaatccgc gatgccctga cgggtgaact gtttcgacag
    24661 gggtaacata atggaaacct atgctgtttt tggtaatccg atagcccaca gcaaatcgcc
    24721 attcattcat cagcaatttg ctcagcaact gaatattgaa catccctatg ggcgtgtgtt
    24781 gacacccatc aatgatttca tcaacacact gaacactttc tttagtgctg gtggtaaagg
    24841 tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc agagcggatg agcttactga
    24901 acgggcagcg ttggctggtg ctgttaatac cctcaagcgg ttagaagatg gacgcttgct
    24961 gggtgacaat accgatggta taggtttgtt aagcgatctg gaacgtctgt cttttatccg
    25021 tcctggttta cgtattttgc tcatcggcgc tggtggagca tctcgcggcg tactactgcc
    25081 actcctttcc ctggactgtg cggtgacaat cactaatcgg acggtatccc gcgcggaaga
    25141 gttggctaaa ttgtttgcgc acactggcag tattcacgcg ttgggtatgc acgaactgga
    25201 aggtcatgag tttgatctca ttattaatgc aacatccagt ggcatcagtg gtgatattcc
    25261 ggcgatcccg tcatcgctca ttcatccagg catttattgc tatgacatgt tctatcagaa
    25321 aggaaaaact ccttttctgg catggtgtga gcagcgaggc tcaaagcgta ctgctgatgg
    25381 tttaggaatg ctggtggcac aagcggctca tgccttcctt ctttggcatg gcgttctgcc
    25441 tgatgtagaa ccggtcatca aactattgca acaggagttg tcagcgtgaa tcaggccatc
    25501 cagtttccgg atagggaaga gtgggacgag aataaaaaat gtgtatgttt tcccgctctc
    25561 gtgaatggta tgcaactgac atgtgcgatc tctggcgaga gtctggcgta tcgctttact
    25621 ggagatacac cagaacagtg gttagagagt tttcgtcagc atcgctggga cctggaagaa
    25681 gaagcggaaa acttaattca ggaacaaagt gaagatgatc aaggttgggt gtggttaccc
    25741 tgatccatat attcgtcctt ccatttcacg tagttatttg cggaatagcg taacccagcc
    25801 ttctcttcat cacttaacgg gcggatctgt ttgacggggc taccgagata cagatatccg
    25861 ctctccagcc gtttattttg tgggaccagg ctacctgcac caatcatcac atcatcttct
    25921 actattgcgc catcaagtaa aattgagccc atcccaacca aaactcgatt gccaatggtg
    25981 cagccgtgga gcatcacctt gtgaccaata gtgacatctt cgccaatggt taatgggttg
    26041 ccagctgggt tgtacgagga tttatgagtg acgtgcaaca tactgccatc ctggatattg
    26101 gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa tcacaacgag cggccagatc
    26161 cccacatcat cagccagacg aacgtcacca atcacgacgc tgctatcgtc gatcattacg
    26221 cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta aaacctcaga catactcacc
    26281 tcaacaataa atgatttact aatgactttg ggtacattat tggccttgtg caagtctttt
    26341 agtatgcaaa aaagcaccgt tttgtgtgcg aatgcagcaa aaagggtgaa aaaacaacaa
    26401 acagaaaaaa agatcaaaaa agtacttgtg caaaaaattg ggatccctat aatgcgcctc
    26461 cgttgagacg acaacgtgaa acacttcaca agatagtcgt aacaacaaag agaaaaatcc
    26521 tgaaattcag ggttgactct gaaagaggaa agcgtaatat acgccacctc gcgacagtga
    26581 gctgaaaacc gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc
    26641 gaagatacgg attcttgacg tcgcaagacg aaaaatgaat accaagtctc aagagtgaac
    26701 acgtaattca ttacgaagtt taattcacga gcatcaaact tttaaattga agagtttgat
    26761 catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga
    26821 agcagcttgc tgctttgctg acgagtggcg gacgggtgag taatgtctgg gaaactgccc
    26881 gatggagggg gataactact ggaaacggta gctaataccg cataacgtcg caagaccaaa
    26941 gagggggacc ttagggcctc ttgccatcgg atgtgcccag atgggattag ctagtaggtg
    27001 gggtaa
//
LOCUS       DAPQZD010000054        26757 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPQZD010000054 DAPQZD010000000
VERSION     DAPQZD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.054"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            26..1069
                     /locus_tag="RO454_002238"
     CDS             26..1069
                     /locus_tag="RO454_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDY0934068.1"
                     /translation="MQGIKIYTILASSLISGSVFAQTEISTSNSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            complement(1212..2576)
                     /locus_tag="RO454_002239"
     CDS             complement(1212..2576)
                     /locus_tag="RO454_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDY0934069.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2665..3441)
                     /locus_tag="RO454_002240"
     CDS             complement(2665..3441)
                     /locus_tag="RO454_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDY0934070.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            complement(3446..4084)
                     /locus_tag="RO454_002241"
     CDS             complement(3446..4084)
                     /locus_tag="RO454_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDY0934071.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(4081..5343)
                     /locus_tag="RO454_002242"
     CDS             complement(4081..5343)
                     /locus_tag="RO454_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDY0934072.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5340..6248)
                     /locus_tag="RO454_002243"
     CDS             complement(5340..6248)
                     /locus_tag="RO454_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDY0934073.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6444..7211
                     /locus_tag="RO454_002244"
     CDS             6444..7211
                     /locus_tag="RO454_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0934074.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(7262..7918)
                     /gene="pphB"
                     /locus_tag="RO454_002245"
     CDS             complement(7262..7918)
                     /gene="pphB"
                     /locus_tag="RO454_002245"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDY0934075.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(8024..10585)
                     /gene="mutS"
                     /locus_tag="RO454_002246"
     CDS             complement(8024..10585)
                     /gene="mutS"
                     /locus_tag="RO454_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDY0934076.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            10871..11215
                     /locus_tag="RO454_002247"
     CDS             10871..11215
                     /locus_tag="RO454_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDY0934077.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(11254..11682)
                     /locus_tag="RO454_002248"
     CDS             complement(11254..11682)
                     /locus_tag="RO454_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDY0934078.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(11727..13805)
                     /gene="flhA"
                     /locus_tag="RO454_002249"
     CDS             complement(11727..13805)
                     /gene="flhA"
                     /locus_tag="RO454_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDY0934079.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(13879..14889)
                     /gene="hypE"
                     /locus_tag="RO454_002250"
     CDS             complement(13879..14889)
                     /gene="hypE"
                     /locus_tag="RO454_002250"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDY0934080.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(14886..16007)
                     /gene="hypD"
                     /locus_tag="RO454_002251"
     CDS             complement(14886..16007)
                     /gene="hypD"
                     /locus_tag="RO454_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDY0934081.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(16007..16279)
                     /gene="hypC"
                     /locus_tag="RO454_002252"
     CDS             complement(16007..16279)
                     /gene="hypC"
                     /locus_tag="RO454_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDY0934082.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(16270..17142)
                     /gene="hypB"
                     /locus_tag="RO454_002253"
     CDS             complement(16270..17142)
                     /gene="hypB"
                     /locus_tag="RO454_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDY0934083.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(17146..17496)
                     /gene="hypA"
                     /locus_tag="RO454_002254"
     CDS             complement(17146..17496)
                     /gene="hypA"
                     /locus_tag="RO454_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDY0934084.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            17708..18169
                     /gene="hycA"
                     /locus_tag="RO454_002255"
     CDS             17708..18169
                     /gene="hycA"
                     /locus_tag="RO454_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDY0934085.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            18294..18905
                     /gene="hycB"
                     /locus_tag="RO454_002256"
     CDS             18294..18905
                     /gene="hycB"
                     /locus_tag="RO454_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDY0934086.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            18902..20728
                     /gene="hycC"
                     /locus_tag="RO454_002257"
     CDS             18902..20728
                     /gene="hycC"
                     /locus_tag="RO454_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDY0934087.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            20731..21654
                     /gene="hycD"
                     /locus_tag="RO454_002258"
     CDS             20731..21654
                     /gene="hycD"
                     /locus_tag="RO454_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDY0934088.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            21672..23381
                     /gene="hycE"
                     /locus_tag="RO454_002259"
     CDS             21672..23381
                     /gene="hycE"
                     /locus_tag="RO454_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDY0934089.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            23391..23933
                     /gene="hycF"
                     /locus_tag="RO454_002260"
     CDS             23391..23933
                     /gene="hycF"
                     /locus_tag="RO454_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDY0934090.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            23933..24700
                     /gene="hycG"
                     /locus_tag="RO454_002261"
     CDS             23933..24700
                     /gene="hycG"
                     /locus_tag="RO454_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDY0934091.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            24697..25107
                     /gene="hycH"
                     /locus_tag="RO454_002262"
     CDS             24697..25107
                     /gene="hycH"
                     /locus_tag="RO454_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDY0934092.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            25100..25567
                     /gene="hycI"
                     /locus_tag="RO454_002263"
     CDS             25100..25567
                     /gene="hycI"
                     /locus_tag="RO454_002263"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDY0934093.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            25592..25777
                     /locus_tag="RO454_002264"
     CDS             25592..25777
                     /locus_tag="RO454_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934094.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            25803..26222
                     /locus_tag="RO454_002265"
     CDS             25803..26222
                     /locus_tag="RO454_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0934095.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(26314..26751)
                     /locus_tag="RO454_002266"
     CDS             complement(26314..26751)
                     /locus_tag="RO454_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934096.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
BASE COUNT         6203 a         7010 c         7064 g         6480 t
ORIGIN      
        1 acaacacatt aataaaggac tacgtatgca aggcattaaa atatatacaa ttctagcctc
       61 ttctcttata tctggtagtg tttttgctca aacggaaatt tcgacttcaa attcgtctac
      121 agttaatgca agttatgttg aaccttcagc ggaaaaaata atatcgccaa gcgataaact
      181 caataacctt tttgaaagaa atatgtctca gccttatatt ttacaaaaaa ttggcgagag
      241 aacttattat gttcaacgct atttttattc cactacgttt tatgtcggag acaaaggggt
      301 tttgctgttt gatgctccgg agggtcgcgg taaatattta ttacaggcaa ttcgtgatgt
      361 gactccatta cccgtaacag cgctggttta ttctcattac catgtggatc acattggcga
      421 tagtccattt tggaacgatg aggcgaagaa agaaggcgtg aatctgcgta ttattgccag
      481 taaagccact gctgaaaaaa tgcaatttat gaacagtcgc ttacccgtcg ccactcaggt
      541 tttaagtaaa aaagatgacc agtttaaatt tgaaaagcag accattgaat tacaccgatt
      601 tgttaaagca ggacataccg acgaccattc tgtatggttg ctaaaacagg aaaaagtagc
      661 tcacagcccg gatctcctga atcctgacca actgccgatg atgggcttcg ccgtatcgga
      721 tacgttggtt taccatgaca gtaatttgcg tcaggttgaa atgcttgact ggaaatattt
      781 tattggcggg catggtaata ttggttcaca cgatgatttt aaatttcaac gccagtttct
      841 taatgatctt cgggatacga caattaaagt cagaaaagaa gaaagttttg gtaaatttat
      901 gaataaaacg gcaaataatc atgctgattt tgcgcgagcc cagcgcgaag caattattaa
      961 aaaagtaact gaagtattac gtccgaaata tggccatatg tatggctacg acgcatctat
     1021 gcctgctaat atcgaaatgg caataaggtt ggtcggttct tattactaag ataaaatcaa
     1081 cttgcaagag actgtaatta tattgcaaag tgaaaattaa cctcggtagc gagatattac
     1141 cgaggttaat attggcatac attacaccct tatcaggcac ttaaacgata catcatatct
     1201 tatctggatt attagatgac gagccatacg caccaggtaa ttaagaatcc ggtaaaaccg
     1261 agaatggtcg ttaacactgt ccaggttttc agaccgtctg ctaccgacaa ccccagatat
     1321 ttggtcacaa tccagaaccc tgagtcatta atatgtgacg cgccaagccc accaaagcag
     1381 gctgccagcg tcaccaatac gcactgaatt ggattcaatc ccatcaccgc ttctgagagt
     1441 aacccgccgg ttgtcaggat tgctacggtt gctgacccct gcgatgcacg cagcgccagt
     1501 gaaataataa atgcggctgg taacagaggc aggtcaatca tttgtaacat gttggcaagg
     1561 gctttaccga cgcccgattc caccagcact ttgccaaata cccctccagc accagtaacc
     1621 aaaatcacta ccgccgcagt gggaagggca gagcccataa tgtcgctggt gtgttgtaag
     1681 ctccagccgc gacgtaaagc caataaccag aatgccagca ccagcgcaat cattagagct
     1741 accattggtg agccgatcaa ctgtagcgta ccaagcaggg gatgcgaagg cggcatcagt
     1801 gttgcagaaa ccgttcccgc catgataatc gcgataggaa taacaattag cgaggtgact
     1861 aacgcgacgc ccggtggatt tattttatcg cttaattttg tcgcgccttc ctcactggcc
     1921 ggagccagtt gcatctgttc cagtacctcg accgacatcg catattggcg cttattgatt
     1981 attttcgctg caaagtagcc aacaatccct acgggaatag aaatcgcaat accgatgatg
     2041 gttagccagc cgatgtctgc gtggagtaat cccgctgcgg cgacagggcc tggatgcggc
     2101 ggtaccgcca cgtgaacagt gagcatgatc ccagcgacag gcaggccaaa tttgagtggt
     2161 gatattttgg caaccttggc aaaaccgtaa atgattggcg caagaataat aaagccgaca
     2221 tcaaagaaga cgggaatgcc gaggaagaac gctgccagag tcagcgcagc gatagttcgt
     2281 ttgtcaccta acttgcgact gaaataatta gccagtgact ctgcaccacc agagtgttcg
     2341 atcatacgcc ccagcatagc gccaagacca ataataatag tgacggaacc aagcacaccg
     2401 cccatcccgg cgatcatcac tttacccact tcgcccgccg gtatacccgc cgcaagtgcg
     2461 actaacaggc tgacgaggag cagagcaacg aatggttgta cctttgcctt gatgaccagt
     2521 agcaacagca tgattacgcc agctaacgca atgcataaca atgtaattgt ggacatggga
     2581 aaccctgtct gaaagttata gttaacctac cccatccgta gatgggggga tgtatgggta
     2641 cgttgtaatt agggattgag cgaattagcg ccaggcgtca aaccagccaa gcccttcttt
     2701 ggtgaggcca cgaggtttgt attcacaacc gatccagccc tgatatccca cttcatcgaa
     2761 caggcggaac agccacggat agttgatttc tccatcgtcc ggttcatgtc gatcaggtag
     2821 tccggcaatt tgtacgtgcg catatttccc ggcgtagttg cggattaaat gtgtcaggtt
     2881 gccatctact ttttgcgcat ggaaagtatc tagttgaata aacacgttat ctcgcgcaac
     2941 ctcttcaaca atagccagtg cctgatactg gctggagaag agataatgag gcttaacgcc
     3001 ggggctgagt gcttcaacca atattcgctt gccgtgtggc gcaaagcggt cggcagcgta
     3061 gcggagatta tcaataaata ctgcccggta ccgttcagca tcttcgccag cgggcacgac
     3121 gcctgccatc acatggactt gttcacaatt gagcgccaat gcatattcca gtgccaggtc
     3181 gatgtctgcg cgtgcttcgt gctcacgtcc gggaagggcg gataatcccc attctcccgc
     3241 attaatatct ccgggagcgg tattgaacag cgccagtgtc agatggtttt gcaccagttg
     3301 ctgctggatt tgctgggggg agtagtcata gggaaacaga aactccacag catcgaaccc
     3361 ggcttttcgc gctgcggcga agcgttcaat aaaaggcact tcggtgaaca tcatggataa
     3421 attagctgca aaacgaggca ttgcattaac tccttaattc cgcaatttca cctgcggtca
     3481 gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc tccagctctt
     3541 ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca tgatttgcca
     3601 gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg cgtttatcgc
     3661 ccggtcggta atagggcacc agcgggacgt ttcccatccg catcaccacg tatggtgtga
     3721 acggacgaat aacgttattg ctgtccagcc cttccaggca ggaaagcgcc gtcgaccacg
     3781 tactgtgcaa atgcaccacc gctttacaga gcggattgtt gcgatacagc gccagatgaa
     3841 agagcacctc tttcgagggg ttgtcaccac ttaaccattc gccatccgcg gtgactttgg
     3901 aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc agtaaattcc
     3961 cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatatccg cgctgaaaga
     4021 atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt gcgaaatcgc
     4081 tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga agttgccaga
     4141 tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc cgggggaaat
     4201 ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc cggaggtctc
     4261 accgcctgcg acaataaagc gtgtcacgcc ttccgctgct aaccgcgccg ctagttgaga
     4321 aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt gaattgctgc
     4381 caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt cctggcccag
     4441 aacccactct gccagctcgt gtgcataagc ggccagagtt tcggttgaga ggcagcgtgc
     4501 cacatcaacc tcacgggctg gtgcaatttg acggtaatgt gccacctggc ggttggtcat
     4561 ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc ctgcttcgcg
     4621 agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca gaccagaacc
     4681 gcccgttacc agtggggcat cgcgcaaggc ttccccctga atttccagat ggtgttcggt
     4741 cagcgcatca agcaccgcgt agcgatagcc ctcttgctgt aagcgagcca gctcctgacg
     4801 aacggctttc acaccttgtt cgaaaacatg tgctgaaacg acgccgcagc gccctgtgga
     4861 ttgcgcttca accagacggg gaagatagct gtcggtcatg ggatttaccg ggtgatggcg
     4921 cattccggat tcggccagca gttgattcat tacgaacaaa tacccctgat aaaccgtacg
     4981 tccgttgact ggcagggccg gagagaagac cgtaaacggt gtgtcgagag catccattaa
     5041 tgcatcggta actgggccga tattcccttt cgccgtactg tcgaaagtag agcagtattt
     5101 gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca gcgattgctg
     5161 tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg cgtcgattgc
     5221 ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac cgttttccac
     5281 cagaaaactg gcgatatccg tcgcgccggt aaaatcatca gcgataacgc caatcttgat
     5341 catgatttcg ctcccggtag ggtgatgcca gagaaaatct tgataactgc gctatcgtct
     5401 tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt tgaggccaat
     5461 ggcagcggga agtgcagggc tttggcggta tcggcaacca gaccaagatc cttaacaaaa
     5521 atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat ccggttttcg
     5581 aacatccatg aatttccggc ggcattggtc acgacgtcat acatcacatc cagcgggatc
     5641 cccgcacggg ctgcaagcgc catcgcttcg gctccggcag caatatgtac gcccgccaac
     5701 aactggtgga taatttttac ggtcgaaccc agtcccggtt ctgctccgat gcgataaact
     5761 tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc gctaccggag
     5821 gccatgacag tcatttcacc gttagcggct tttaccgcac caccagaaac cggcgcatcc
     5881 agcatttcca gaccgaatcc agccagagcg gtagcaattt cttgcgcatc agcattggcg
     5941 atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac gcctttttca
     6001 ccaaacagca cctgtttaac ctgggtcgca ttgaccacca gcaccagcag agcgtccagt
     6061 ttttcggcaa acgtcgcggc gttatcagaa accccgcaag caccagcctc tttcaacgta
     6121 gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa gacctgcgcg gacacatgac
     6181 agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa ctccgatccc
     6241 gttttcatct gctctccttg ttaatttaag tgatattttg tttgatattg tgaatataag
     6301 cgcggaaaga taacgatatg gtgagctgat tcacaaaaat taacattgtg tgttatttta
     6361 tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt agaatgtcag
     6421 caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc atccttgaga
     6481 tggtagctga aaaaggcatt gttagtattg ctgaactaac ggacagaatg aatgtgtcac
     6541 atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt gtgctggtgt
     6601 ccgggggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag gtaaaaactg
     6661 cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt gttcagccgg
     6721 gacgttgtat ctatctggat gcgggaacga ccacgttagc gattgcacag catctgattc
     6781 acatggagcc actcacagtg gttactaacg acttcgttat tgcggactac ttgctcgaca
     6841 acagtaattg cacaattatt cacactggcg gcgcagtgtg tcgggaaaac cgttcctgtg
     6901 ttggggaagc cgctgcgacc atgctgcgta gcctgatgat tgatcaggct tttatttctg
     6961 catcgtcatg gagtgtgcgg gggatctcta cgccagcaga agataaagtc acggtgaaac
     7021 gggcgattgc cagtgccagc cgccagcgag ttttggtctg tgatgcgacg aaatatggac
     7081 aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagatcatt actgacgacg
     7141 gtctgccgga gagtgccagc cgcgcgctgg cgaagctgga tctctctttg ctggtagcga
     7201 aaaatgaata atgacctgca ataacgccgg gttactcatg cttcacagaa gaagcatgag
     7261 actactttat tttataaaat gacagccgcc cgcttttcgg cgatccggta tctatataaa
     7321 tctggttagc gaacgtctga atgttatcaa acatcatatg accaaatata aagaagtcgg
     7381 cgccgtttat tttttgtaat tcgctattaa gtgatttctg cacacgatca acaggccaga
     7441 gtaattcgct ctccgctatt tctttgccaa actgatactc cttccccgga taatctgcat
     7501 gtgcgatgac atattttatg ttgtcgttag tgatttcaat aatatgtgga aggagatgga
     7561 atttcagcaa cagatctatt gcctcttgtt gctctgaatc atttaaatcg aaaaaccagt
     7621 caccaccgct ggcaagccac atatttccat cgccagtttc gaatgcctca agcgccatcg
     7681 cttcgtggtt gcctttgacg gagataaacc agggctggtt taacaggcgc agcacgttaa
     7741 gactctccgg cccacgatcg atgttatcgc ctaccgaaat aagtaagtcg gtttcaggac
     7801 aaaaagagag ttgatgtaag cgggattgta ataattgata gtcaccatga atatcaccaa
     7861 cggcccatat atggcgatag tgatgggcat tgattttttg atagcgtgta gatggcatgg
     7921 ttttaccctg taaaataagt tttcctatta tacagggtat ttttatttga ttcgtcagtt
     7981 gtcgttaata ttcccgatag caaaaggcta tcgggaatta ttattacacc agactcttca
     8041 agcgataaat ccattccagc gcctgacgcg gagtcagtga atccgggtcg aggttttcca
     8101 gtgcctcgac cgcaggcgaa gtttcttccg gtacggacag caaagacatt tgtgtaccat
     8161 ccacttgcgt agcagcggcg ttcggcgaaa tgctttccag ctcacgcagt ttttgccgtg
     8221 cgcgcttaat aacctctttt ggcacacccg ccagagctgc aaccgccagg ccgtagcttt
     8281 tgctcgccgc gccatcctgc acgctatgca taaaggcaat ggtgtcgccg tgctccagtg
     8341 catcgagatg cacgttggcg acgccttcca ttttctccgg taactgggtc agctcgaaat
     8401 agtgggtagc aaacagcgtc aacgctttga tcttatttgc cagattttcc gcacacgccc
     8461 acgccagcga cagaccatcg taagtggacg ttccacgccc gatctcatcc atcaacacca
     8521 gactatattc ggtggcgtta tgtaaaatat tggcggtttc ggtcatctcc accataaagg
     8581 ttgaacgccc ggaagccaga tcatccgcag cacctacgcg ggtaaagata cggtcaatcg
     8641 ggccaatctc gactttttgc gccggtacgt agctgccgat atacgccatc agcgcaatca
     8701 acgcggtctg gcgcatatag gtacttttac cgcccatgtt cggaccggta atgatcaaca
     8761 tgcgacgctg cggcgacagg ttcagcgggt tagcgataaa cggctcattc agtacttgtt
     8821 caactaccgg atggcgacct tcggtaatac gaatgccagg tttatcaatg aaggtcgggc
     8881 aggtatagtt cagggtatag gcccgttccg ccaggttcac cagcacgtcg agttccgcca
     8941 gcgcgctcgc gctctgttgc aacgcttcca gatgcggcaa cagcaggtcg aacagctctt
     9001 cataaagttg tttttccaga gccagtgctt tgccttttga ggtgagaact ttatcttcgt
     9061 actcttttag ctctggaatg atgtagcgct cggcgttttt cagcgtctgg cgacgcatgt
     9121 agttgattgg tgccaaatgg ctttgcccac ggctgatttg aatgtagtag ccgtgcaccg
     9181 cattaaagcc aactttcagc gtgtccaggc cggtacgttc acgctcgcgg acttccagac
     9241 gctccagata atcggtcgcg ccgtcagcca gcgcgcgcca ctcatccagc tcttcgttat
     9301 agcccgatgc gataacacca ccgtcgcgta ccagcaccgg tggtgtgtcg atgattgctc
     9361 gctccagcag atcgcgcagc tcggcaaact cgcccatctt ctcacgcagc gcttgtaccg
     9421 gtgcactatc gacattttct aactgcgcac gcaactccgg cagttgctgg aaagcgtgac
     9481 gcatacgggc cagatcgcgt gggcgagcgg tacgcaacgc cagacgcgcc agaatacgtt
     9541 ccaggtcgcc cacctgacgc agtaccggct gcagctcggc ggtgaaatcc tgcaatgcgc
     9601 caatagtttg ctgacgctca agcaacacgc gggtatcgcg cactggcata tgcagccagc
     9661 gtttcaacat acgactaccc atcggcgtta cagtgcagtc gagcactgaa gccagcgtat
     9721 tttccgcacc gccggccagg ttctgagtaa tttccaggtt acgacgcgtc gcggcatcca
     9781 taatgatgct gtcctgctga cgttccatag tgatagaacg gatatgcggc agggtcgtgc
     9841 gttgggtatc tttcgcatac tgcaacagac aaccggcagc acaaagtccg cgtggtgcgt
     9901 tctccacacc aaaaccgacc agatctcggg tgccaaattg caggttcaat tgctggcgag
     9961 cggtgtcgat ttcaaactcc cacagcgggc gacgacgcag gccgcggcga ccttcaatca
    10021 gcgacatctc ggcgaaatct tctgcataca gtaattccgc tggattcgta cgttgcagtt
    10081 ctgccgccat cgtttcgcgg tccgccggtt cgctcaggcg aaaacgaccg gaactgatat
    10141 ccagcgttgc gtagccgaaa cctttactgt cctgccagat agccgccagc aggttgtcct
    10201 gacgttcctg caacagcgct tcatcgctga tggtgcccgg cgtaacgata cggacaactt
    10261 tgcgctcgac cggccctttg ctggtcgccg gatcgccaat ctgttcacaa atagcgacgg
    10321 attcgccctg attcaccagt ttggcgaggt agttttccac cgcatggtag ggaatccccg
    10381 ccatcgggat tggctctccc gctgaagcac cgcgtttggt cagtgaaata tccagcagtt
    10441 gcgacgcccg ttttgcgtcg tcataaaaca gttcataaaa gtcgcccatc cggtaaaaca
    10501 gcaggatctc gggatgctgg gctttcagct tgagatactg ctgcatcatg ggcgtatggg
    10561 cgtcgaaatt ttctattgta ctcatggggt taagtccggc tccctgatgt taaatgtagt
    10621 gtgatggttt ttattttcgt tgtaatcaca taaggcgcac atgataccgg agttaatcat
    10681 ttcaggggaa gacgcgaaag gtgagttctt gatgtcggtt ccgcgaaaaa tgctgctttg
    10741 cagcttttgc gtgatgacga tgaaacaaga tcgccaaatt cgtcggttga agaaacctcc
    10801 tccttcagag ccatagtccc ctttaaaatt aagttgtaat ataaatgcac cttaagagta
    10861 tggaggcagc atgtccggca agcgtatctc tcgtgaaaaa ctgacgatta aaaaaatgat
    10921 cgatctttat caagcgaaat gcccgcaggc gtcagcagag ccggagcatt acgaggcgtt
    10981 gtttgcttac gcgcaaaagc ggctggataa atgtgtgttc ggcgaagaga aaccagcctg
    11041 taagcagtgt ccggtgcact gttaccagcc tgcaaagcgt gaagagatga aacagattat
    11101 gcgttgggcc gggccgagaa tgctgtggcg tcatccgatc ttaaccgtgc gtcatctcat
    11161 cgatgacaag cgtccggtgc cggagttgcc agaaaaatat cgtccaaaga aataaatctg
    11221 ccggagagcc atactctccg gcaaatacgg cgcttacgct ttggtggcaa tcaggatggc
    11281 tgatgctttg atcaatgcaa taacgcgcga gccagtggtg agtttcattt catcctgact
    11341 ttcatttgtt accactgcca caatttcaaa acccgcatcc gttttgatat ggacagtggc
    11401 gttcactgcg ccttcggtga ttgtcgacac gctgccagca aactggttgc gagcagagaa
    11461 tttcagtcca cagtcttccg ttgccagcgt tacccacggg gctttaatca gggcaatcgc
    11521 ttctttccct ttcgccagac ccagcgcttg ctggctgctg tgagtcacaa ttgccaccag
    11581 ctttgcacca cctgccagcg tcagttctac ttcgtcattc accgcgccca ttgctactgc
    11641 gctaactgtt ccggttaatt gatttcttgc tgaaactgcc atactgcctc ctttcattga
    11701 atgttgatcg agtataacca catctgttaa atcaatgccg atttatcaat tcccagtcgc
    11761 ttcattcgtg acagcagagt cgtgcgtttc agccccagac gttgcgcagc gcctttaggc
    11821 ccggcaacca cgccgtttgt ttctttcagc acgcgaacaa tcaactgata ttcatcttcg
    11881 ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttctggtaa agcaatatct
    11941 ggcaatgaca gctgcagcac gttgccgcgt gttagcaata ccgcgcgctc gatgacgttt
    12001 tccagctcac gcacgttgcc cggccattcc atgttgctca ggatgcgcag cgtctcggca
    12061 ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa ggctttcgcc
    12121 agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtggatcgg gaatacgttc
    12181 aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt cagatcgcgg
    12241 ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc gagacgttca
    12301 aactcctgtt cctgtaatac acgcagcaac ttcggctgta actccagcgg catatcgccc
    12361 acttcatcga ggaatagtga gcttttatcc gccagttcaa aacgaccaat gcgctgagcg
    12421 ctggcaccgg taaaagcccc gcgctcatga ccaaatagat cgctttccag caatccggca
    12481 ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact gagattatgg
    12541 atcgcgcggg caatcagctc tttacctgta ccagtttcac cgaggatcag aacggtactg
    12601 tcactttgcg ccaccatttc aacttgttta agtacgctgt acatggcttc gctgcggcca
    12661 ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc gaggttttca
    12721 tcaaccagcc gttctttcag acgatgaatt tcctgatagg cgagggcgtt atcgacagcg
    12781 attgccacac gttcggcaat ctggcgcagt aaattcagat tggtagtggt aaagactttc
    12841 tcttcgcatt gcgccagttt cagtacgccc agcatggtgt cgccagacat cagcggtaac
    12901 aggcacaagg tttgaatctg attgccccag gtgttgaaca acatgcgttc atagggggct
    12961 aaatcgtccc gctcgtggag attaatcaac agcatctctt tacttttgaa gacgcgttcg
    13021 gtgagggttc cggcttcatc gacttcgctc tgttcgtgag cgggatgctg tttatcaaga
    13081 tagtgagtgg agtagatgtt gagtttgttc ttacggtggc tgcgtaagac gatgctgata
    13141 tcgtcaatgt cgaagtagta gtggatttct ttggcgactt cgctgaccag ttcgtccata
    13201 tccaggcggg aaagcaccgc gttagtgatg gcgaccagga tgcggaagtt atcgcgttcc
    13261 cggcataaca actcatagtc gacattgtta acgacgcggc tctggatttg ttcggtgacg
    13321 acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct ccaggggcga
    13381 tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc cagtggcatc
    13441 aggcaatagt gaccaaattt ggggtatagc ccacctgtgg ccagctgcgg ccaggtttca
    13501 caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgct gcgtaccgga
    13561 ccgtgtgcca gaacagtttc gtcttcatat ttaatggggg tgtctttttc acgcgatgcg
    13621 taataagacg cacgttgagt ctgcgcttgc cacaacacaa tcgccgcgtt gtcggcgagc
    13681 gcagaacgct ttaccagttg cgaaagagcc tcacacagcg aggccagatc gggctgctgc
    13741 aatagtgtcc gagtgatgtc gaacaaccct tgctgtccga gatcactcat cggtgtatat
    13801 gacattttgt tcttccagga aagcaccgca aagcggtgca aaaggtcatt gactaactat
    13861 aggagattta gaattttatt agcatatacg cggaagcggt tcggcgtgcg gtagatcgag
    13921 ggttcgtttc acgccataca gaccggcaag acgaacacct ttacgttcta ctacttcgcc
    13981 aatcagcgcc gcatcttttc ccaatggatg ggaatgtaac gccgccagca cttgctctgc
    14041 tgcgttgcgt tcaacggcga tcaccagttt gccttcgttg gcaaagttaa gggcgtccag
    14101 tcccagcagt tcgcaaacgc cgcgcacagc aggtttaacc ggcagtgccg cttctgaaag
    14161 ttcaatacca caaccgcagg ctgccgcgaa ctcatgaacc accgcgttta caccaccacg
    14221 ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc agcgtctgaa taagcggcgc
    14281 cagcaccgcg cagtcgctga ccagttctcc atccagccct agttgctcac gcaggttaag
    14341 gatagtcgcc ccatggtcgc cgagtgtacc gctaaccagc aatacatcgc ctgcggttag
    14401 cgtctgtgcg ccccagtgaa tattcgccgg aattgcgccc atgccagcgg tgttgataaa
    14461 cagtttatct gccgcgccgc gctgtaccac tttggtgtcg ccagtaacga tggcaatgcc
    14521 tgccgcacgg gcggtttctg ccatgctggt cactacggct ttcagtgtct ccatcggcaa
    14581 tccttcttcg aggataaagc cacaggagag atagcgcgga attgcgccac tgaccgcaac
    14641 gtcattggcg gtgccgcaaa tcgccagctt gccgatatta ccgccaggaa agaacagcgg
    14701 gtcaatgacg taactgtcgg tggagaacgc cagacggtcg ccttccgcta ccagctgtgc
    14761 caaatcaaga cgggcctgat cttcctgctc tgccagccac gggttggcaa aggcttccat
    14821 aaacaggctg ttgattaatt gctgcatcgc ctggccgccg ctgccgtggg cgagttggat
    14881 attattcacg cttcactctc ctgctgacga tactgatacc acgcggcgca cgctccttcg
    14941 gaggaaacca tcagcgcacc aaacgcggtt tgaggattac aggtgttacc aaacagcggg
    15001 cattgatgcg gcttacattt gcccgtcaag acttcgccac aacgcgcacg cgggtcatcg
    15061 cagacctgct gcggtgccgg gcggaaatgt gcttcggcat cgaatcgttg ataatccggc
    15121 gtcaggtgca cgccagaaga ttcaatcacg cctaagccgc gccattcact gtcgccgttg
    15181 acacagaaca catcagcaat ggcctgttgc gccagcaggt taccggcatc cggtaccacc
    15241 cgacgatact gattctctac cttgctgtgg gccgctattt tctgctccac cagcatcacc
    15301 acgccttgaa gtagatcaag gggttcgaaa ccagcgacca ctagcggacg atgaaaatcg
    15361 ctggcgataa aattataggc atcagtgccg ataaccatac tgacgtggcc cggcgcgagg
    15421 aacgcgtcga taccgttatc tggctgttcc agcaaactgc gcagtgtcgg gataagcgta
    15481 atgtgctggc agaagaagta aaaattctgc acatcgcggg ctttcgcctg ttgcagagtg
    15541 atggcggtgg tcggcatggt ggtttcaaaa cctaagccga agaataccac tttacgggtt
    15601 ggattctcct gcgccagttt caacgcatcc attggcgagt aaacgatgcg cacatcggca
    15661 ccgcgtgctt ttgcctgcaa cagcgacccc tgtttccctg gtacgcgcat ggcatcgcca
    15721 aaggtacaga agatgacttc cggatggctg gcaatctcca cgcaggtgtc gattctaccc
    15781 attggcagta cgcacaccgg gcaacccgga ccgtggataa actcaacgtt ttccggtagc
    15841 aactggtcga ggccgaattt aaaaatggcg tgggtatgac cgccacacac ttccataatc
    15901 cgcagagggc gttcggcggt gtaagagaga tgtgaagcac gttcgcgcag atgctcaatt
    15961 aactgcatta cctgttccgg cgcgcgatat tcatcaacaa aacgcattat ttttcctcgc
    16021 catacaacag cgcgccgaca tctggctcaa cgtcaaacat gttttgtaag gcgtcgagag
    16081 tgtcgcgtgc ttcggcttca ttaattacgc tcatggcaaa gccaacgtga accagtaccc
    16141 actggcccac gcgtggctga ccgttttcat cgcagctgcc gactaacgtt aaatcgacat
    16201 cgcgttgaat gccgcagacg tcgactttcg cctggttgcc gtcaatggtg cggatctggc
    16261 cgggaacgcc tatgcacatc gctgtgtctc cagccagttc agccactggt ccatcccttc
    16321 gccgctggtg gcggaaataa ggatgatttc aatttctgga ttgacttcac gggcgcaggc
    16381 gatgcacttc tcaacgtcaa agttgagata cggcagcaag tcaactttgt tgagcagcat
    16441 cagcgaggcg gcggcaaaca tgtgcggata tttcagcggt ttgtcttcac cttcggtaac
    16501 ggaaagcacc gccactttgt gtttttcacc gagatcgaag ctggccgggc aaacgaggtt
    16561 accgacgttt tcgataaaca gaataccgtt atcgtccagt ggcaggcgcg gcgcggcgtc
    16621 ggcaatcatc tgtgcgtcaa gatggcagcc tttaccggtg ttcacctgaa tcgctggggt
    16681 gccggtagcg cgaatgcgtg cggcatcgtt cacggtttgc tggtcacctt caataaccgc
    16741 gcacggaacg ctgtctttca ggcgcattaa agtttccgtc agcagggtgg ttttaccgga
    16801 accagggctg gaaaccaggt tgagcaccag ttgcttgcgg gcagcaaagc gcgcgcggtt
    16861 gcgttcagcc agacggttat ttttgtccag cacgtcaatt tcgacttcca gcatccgacg
    16921 ctggctcatg cccggcgcgt gagtgcccgc ttcgccatga ccgtaatgca gatcgccttc
    16981 ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg gtgattttca tcttcgggcg
    17041 tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga gggttatgtt catcaccctc
    17101 gatatagagg ttgccttcac cgcaaccgca tgttgtacac atcgctcact cctggtctat
    17161 ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc tgtagcatgt caccatgaca
    17221 ctgtggacag cggcggacgc gctgggtcag tagcgtcaca tactgttggc atgtttcaca
    17281 ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa ccttccgcca cgctgccgcg
    17341 gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg acacaagaaa atgcgccaat
    17401 tttgagccag accccagtta cgcgttttgc gccgtgtttt gcggcctgct gttcgatcaa
    17461 ttccagtgcc cgttggcaga gggttatttc gtgcatatcg cctcccatta actattgcca
    17521 gctacaagca ataattgtgc cagtgttgat tatccctgcg gtgaataatg tcgatgatgt
    17581 cgaaatgaca cgtcgacacg gcgacgaaat tcatctttag cttagaaatc gctttaataa
    17641 caatgaatta aaagttggca cagaaaatgc ttaaagctgg catctctgtt aaacgggtaa
    17701 cctgacaatg actatttggg aaataagcga gaaagccgat tacatcgcac agcggcatcg
    17761 tcgcctacag gaccagtggc acatctactg caattcgctg gttcagggga tcacgttatc
    17821 gaaagcgcgc ctgcatcacg ccatgagctg cgcgccggac aaagaactct gtttcgtcct
    17881 ttttgaacat tttcgcattt acgtcaccct ggcggatggc tttaacagcc acaccatcga
    17941 gtattacgtc gaaacaaaag atggcgaaga caaacagcgg attgcgcagg cgcagttgag
    18001 cattgacggc atgattgatg gcaaggtcaa catccgcgat cgcgaacaag ttctggaaca
    18061 ctatctcgaa aaaatcgctg gcgtttacga cagcttatat accgccattg aaaacaatgt
    18121 gccggtgaat ttaagccaac tggtaaaggg acaaagcccg gtagcatgag ctgaggcttt
    18181 gcccgttttg caggcgttac gcctgtttgg ggatgggcgt gtcgatgagt gtcgaaaatg
    18241 acatttcatc ggcatgtttt cgtcaaaaat gacaatcacc tgaggaatgc ctggtgaatc
    18301 gttttgtaat tgctgactcc acgctctgta tcggctgcca cacttgtgag gctgcctgtt
    18361 cagagacgca tcgccagcac ggcctgcaat caatgccgcg cctgcgagtg atgctcaatg
    18421 aaaaagaatc tgcgccgcag ctctgtcacc actgtgaaga tgcaccctgc gcaacggtct
    18481 gcccggttaa cgccatcact cgtgttgatg gtgccgtaca gctgaatgaa agcttgtgcg
    18541 taagctgcaa actgtgtggc atcgcctgcc cgtttggcgc aattgaattt tccggcagcc
    18601 gtccgctgga tattccggca aacaccaata ccccgaaagc gccaccggca ccgcctgctc
    18661 cggcgcgtgt cagcacattg cttgactggg tgccaggtat tcgcgcgatc gccgtcaaat
    18721 gtgacctttg tagctttgat gaacaaggtc cggcctgcgt gcggatgtgc ccgactaaag
    18781 ccctgcatct ggtggataac accgatatcg cccgcgtcag caaacgtaag cgtgagctga
    18841 cttttaacac ggactttggc gatctcacct tgtttcagca ggctcaaagt ggagaggcta
    18901 aatgagcgca atttccctga tcaatagcgg cgtggcgtgg tttgtcgccg ccgctgttct
    18961 ggcatttctc ttttcttttc aaaaagcgct aagtggctgg atagccggaa ttggcggcgc
    19021 ggtgggtagc ctgtatacgg cagccgcggg cttcactgtg ctgactggcg cggttggcgt
    19081 gagcggtgcg ctgtcgttgg taagctacga tgtgcaaatc tctccgctta acgcgatttg
    19141 gctgattacg ctcggtctgt gcggtctgtt tgtcagcctc tacaacattg actggcatcg
    19201 ccacgcgcag gtgaagtgca acggcttgca gatcaatatg ttgatggctg ccgccgtctg
    19261 tgccgtcatc gccagcaacc tcggcatgtt cgtggtaatg gccgaaatca tggccctgtg
    19321 tgcggtgttc ctcaccagca acagcaaaga gggcaaactg tggtttgcgc tggggcgtct
    19381 tggcactctg ctgctggcga ttgcctgctg gctgctgtgg cagcgttacg gcacgctgga
    19441 tctgcgcctg ctggatatgc gtatgcaaca gctgccgctc ggttccgata tctggctgct
    19501 cggtgtgatt ggctttggcc tgctggccgg gattattccg ctgcacggct gggtgccaca
    19561 ggcacatgcc aacgcctctg cgccagctgc cgcgctgttt tccacggtag tcatgaaaat
    19621 tggcctgctg ggcattttaa ctctgtcact actgggcggt aatgcaccgc tgtggtgggg
    19681 gatcgcactg ctggtactcg gcatgatcac cgcattcgtc ggcggtctgt atgcgttgat
    19741 ggaacataat atccaacgtc tgctggctta ccacaccctg gaaaatatcg gcatcatcct
    19801 gctggggctg ggcgctggcg taacgggtat cgcgctcgaa caaccggcgc tgattgccct
    19861 tggtctggtt ggtggtctgt accatctgct taaccatagc ctgttcaaga gcgtactgtt
    19921 ccttggtgcg gggagcgtct ggttccgtac cggtcatcgc gatatcgaaa aactcggtgg
    19981 tattggcaag aaaatgccgg ttatctccat cgccatgtta gtcgggctga tggcaatggc
    20041 tgcgctgccg ccgctgaacg gttttgccgg ggaatgggtt atctatcaat ccttcttcaa
    20101 actgagcaat agtggcgcgt ttgttgcccg tcttctcggg ccgctgctcg ccgtggggct
    20161 ggcaattacc ggtgcgctgg cggtgatgtg tatggcgaaa gtttatggcg ttactttcct
    20221 cggcgcgccg cgtaccaaag aagccgaaaa cgccacctgt tcgccgctcc tgatgagcgt
    20281 aagcgtagtg gcactggcga tttgctgcgt aattggcggt gttgctgcgc cgtggctact
    20341 gccgatgctc tctgctgctg tacctctgcc gctggagcct gctaacacca ccgtttctca
    20401 accaatgatc acgttgttgt tgattgcctg cccgctgctg ccattcatca ttatggcgat
    20461 ttgcaaaggc gatcgtttgc cgtcgcgttc ccgcggcgcg gcctgggtgt gcggctacga
    20521 tcatgaaaaa tcaatggtga ttaccgctca cggttttgcc atgccggtga aacaggcgtt
    20581 tgcgccggtg ctgaaactac gcaaatggct gaatccggtg tctctggtgc cgggctggca
    20641 gtgcgagggg agtgcgttgc tgttccgccg gatggcgctg gttgaactgg cggtgctggt
    20701 ggtgattatt gtttcacgag gagcctgaga atgagtgttt tatatccgtt aattcaggcg
    20761 ctggtgctat ttgccgttgc gccgctgctc tccggtatta cccgcgtagc acgcgcccgt
    20821 ctgcataacc gtcgtgggcc gggtgtgttg caggagtatc gcgacattat caaactgctg
    20881 ggccgccaga gcgttggccc ggatgcctcc ggctgggtgt tccgcctgac gccgtatgtg
    20941 atggtgggcg tcatgctgac tatcgccact gcgctgccgg tggtgaccgt cggttctccg
    21001 ctgccgcaac tgggcgattt gatcacctta ctgtatctct tcgccatcgc gcgtttcttc
    21061 tttgccattt ctggtctgga taccggtagc ccgtttaccg ctatcggcgc gagccgtgaa
    21121 gcgatgcttg gcgtgttggt tgaaccgatg ctgctgcttg gtctgtgggt cgccgcacag
    21181 gttgccggtt ccaccaacat cagcaacatc accgacaccg tttatcactg gccactgagc
    21241 cagagcatcc cgctggtgct ggcgctttgt gcctgtgcat tcgccacctt tatcgaaatg
    21301 ggcaaactgc cgttcgacct ggcggaagcc gagcaggagt tgcaggaagg tccgctctct
    21361 gagtatagcg gcagcggctt tggcgtcatg aaatggggta tcagcctgaa acagctggtg
    21421 gtgttgcaga tgttcgtcgg ggtgtttatt ccgtggggac aaatggaaac cttcaccgtc
    21481 ggtgggctgc tgctggcgct ggtgattgcc atcgtcaaac tggtggttgg cgtcctggtt
    21541 atcgcgctgt tcgaaaacag catggcccgt ctgcgtcttg atattactcc gcgcattacc
    21601 tgggccgggt ttggctttgc atttttagcg ttcgtctcct tgctggcggc gtgattaaag
    21661 agagtttgag catgtctgaa gaaaaattag gtcaacatta tctcgccgcg ctgaatgagg
    21721 catttcctgg cgtcgtgctg gaccacgcct ggcagaccaa agatcagctg accgtgaccg
    21781 taaaggtgaa ctacctgccg gaagtggtgg agtttctcta ctacaagcag ggtggctggc
    21841 tgtcggtgct gtttggtaac gacgaacgca aactgaatgg tcattacgcc gtttattacg
    21901 tgctgtcgat ggagaagggc accaagtgtt gggtaacggt tcgcgttgaa gttgacgcca
    21961 acaaaccgga gtatccttcc gtgacgccgc gcgttccggc ggcggtgtgg ggcgagcgtg
    22021 aagtgcgcga tatgtacggt ttgattccgg ttggtctgcc ggatgaacgc cgtctggtgc
    22081 tgccggatga ctggccggat gaactttatc ctctgcgtaa agacagcatg gattatcgtc
    22141 agcgtccggc accgactacc gatgctgaaa cctacgagtt catcaacgaa ctgggcgaca
    22201 agaaaaacaa cgtcgtgccg attggtccac tgcacgtcac ttctgacgaa ccgggtcact
    22261 tccgtctgtt cgtcgatggt gaaaacatta tcgacgccga ctaccgcctg ttctacgtcc
    22321 atcgcggcat ggaaaaactg gcggaaaccc gtatgggtta caacgaagtg accttcctct
    22381 ctgaccgtgt gtgcgggatc tgcggctttg ctcacagcac cgcctatacc acgtcggtgg
    22441 aaaacgcgat gggtattcag gtgccagaac gcgcgcagat gatccgcgcc attctgctgg
    22501 aggtagaacg cctgcactcg catctgctca acctcggcct ggcctgtcac tttaccggct
    22561 ttgactccgg ctttatgcag ttcttccgcg tgcgtgaaac ctccatgaaa atggcagaga
    22621 tccttaccgg tgcgcgtaaa acctacggcc tgaacctgat cggcgggatt cgtcgcgatc
    22681 tgctgaaaga cgacatgatt cagacccgcc agctggcgca acagatgcgt cgtgaagtgc
    22741 aggagctggt ggatgtgctg ctgagcacac cgaacatgga acagcgcacc gtcggcattg
    22801 gtcgtctgga cccggaaatt gcccgcgatt tcagtaacgt tggcccgatg gtccgcgcca
    22861 gcggacacgc tcgcgatacc cgcgccgatc acccgtttgt tggttatggc ctgctgccaa
    22921 tggaagtcca cagcgagcag ggctgcgacg ttatttcccg tctgaaagtg cgtatcaacg
    22981 aagtctatac cgcgctgaat atgatcgatt acggtctgga taacctgccg ggcggcccgc
    23041 tgatggtgga aggctttacc tacattccgc accgtttcgc gctgggcttt gccgaagcgc
    23101 cgcgcggcga cgatatccac tggagcatga ccggcgacaa ccagaagctg taccgctggc
    23161 gctgccgtgc cgcgacctac gcgaactggc cgaccctgcg ctacatgctg cgtggcaata
    23221 ccgtttccga tgcgccgctg attatcggta gcctggaccc ttgctactcc tgtaccgacc
    23281 gcatgaccgt ggtcgatgta cgtaagaaga agagcaaagt ggtgccgtac aaagaactcg
    23341 agcgctacag cattgagcgt aaaaactcgc cgctgaaata aggaatcgcc atgtttacct
    23401 ttatcaaaaa agtcatcaaa accggcacgg cgacttcgtc ttatccgctg gagccgattg
    23461 cggttgataa aaacttccgt ggtaagccag agcagaaccc gcagcagtgc atcggctgtg
    23521 cggcctgcgt caatgcctgc ccgtcaaacg ccttaacggt tgaaactgac ctcgccacag
    23581 gagagctggc gtggcagttc aatcttggac gctgcatctt ctgtggacgc tgcgaagaag
    23641 tctgcccgac ggcggcgatt aagctgtcgc aagaatacga actggcggtg tggaagaaag
    23701 aagacttcct gcaacagtcc cgcttcgcgc tgtgcaactg ccgcgtctgc aatcgtcctt
    23761 tcgccgtcca gaaagagatc gactacgcca ttgcgctgct taagcacaac ggcgacagcc
    23821 gcgcggaaaa ccaccgcgaa agctttgaga cttgcccgga atgtaagcgc cagaaatgcc
    23881 tggtgccgtc cgaccgtatt gaactgactc gccatatgaa agaggccatc tgatgagcaa
    23941 tttattaggc ccccgtgacg ccaacggcat tccggtcccg atgacggtgg atgaatccat
    24001 cgccagcatg aaggcgtcgt tactgaaaaa aatcaaacgt tctgcctacg tttatcgcgt
    24061 ggactgcggc ggctgtaacg gttgcgaaat cgaaattttc gccacgcttt cgccgctgtt
    24121 cgatgcagaa cgcttcggca ttaaagtcgt tccgtcaccg cgtcatgcgg atattttact
    24181 gtttaccggc gcggtcaccc gtgcaatgcg atcccctgcg ctgcgtgcgt ggcagtccgc
    24241 gccggacccg aaaatctgta tctcttacgg tgcctgcggt aacagcggcg ggatcttcca
    24301 cgatctctac tgcgtgtggg gcggtacgga taaaatcgtt ccggtagatg tctacatccc
    24361 tggctgcccg ccaacgcctg ccgccacgct gtacggcttt gcgatggcgc tcggcctgct
    24421 ggagcagaaa atccacgccc gtgggccggg tgagcaagat gagcagccgg cagacattct
    24481 acatcctgat atggtgcaac cgttgcgtgt gaaagtggac cgtgaagcgc gtcgtctggc
    24541 gggttatcgt tacggtcgcc agattgccga tgatttcctg acacagttag ggcagggcga
    24601 agaacaggtt gcacgctggc tggaagcgga aaacgatccg cgtctgaacg agattgtcag
    24661 ccatctgaat catgttgttg aagaggcgcg tatccgatga gtgaaaaggt ggtgttcagt
    24721 caactgagcc gtaaatttat tgatgagaac gatgccacgc ccgccgaggc gcagcaggtg
    24781 gtctattaca gcctggcgat tggtcaccac cttggggtta tcgattgcct ggaagcggca
    24841 cttacctgcc cgtgggatga ctatctggca tggattgcca ctctggaggc gggcagcgaa
    24901 gcccgccgta aaatggaagg cgtgccgaaa tatggtgaga tcgtcatcga cattaaccat
    24961 gtgccgatgc tggccaacgc attcgataaa gcccgggcag cgcaaacttc gcagcagaag
    25021 gaatggagta caatgctgtt aagtatgctg catgatattc atcaggaaaa cgccatctat
    25081 ttgatggtga ggagactgcg tgactgacgt tttactttgt gttggcaata gcatgatggg
    25141 cgatgatggc gcaggcccgc tactggcgga aaagtgcgcc gccgcgccga aaggtaactg
    25201 ggtagtgatt gacggcggta gcgcaccgga aaacgacatt gtcgctatcc gcgaactgcg
    25261 cccgacacgg ctgctgattg tcgacgccac ggatatgggg ctaaaccctg gcgagatccg
    25321 catcatcgac ccggctgaca tcgccgagat gtttatgatg actacccata acatgccgct
    25381 gaactatctt atcgaccagt tgaaagaaga tattggcgaa gtgattttcc ttggcatcca
    25441 gccggatatc gtcggctttt actacccgat gactcagccg attaaagatg cggtggaaac
    25501 ggtgtatcag cggctggaag gctgggaagg aaatggcggc ttcgcgcagt tagcggtgga
    25561 agaataattc ttcattaggg aattcaggga attgaacatc aaaacaggga tgtgcgcttt
    25621 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    25681 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    25741 cgagcaaacc tggcatttta gaaaagtggg cgtctgacac gttactacag agagaacaaa
    25801 gaatgactgc taatgctgca cgcgctgtta aagcaacaag agaactggtc aatgcagtcc
    25861 cctttctggg aggcagtgac tcagaagatg attaccgtga agcactggaa ctggtcgaat
    25921 atttgattga agaagatgat actaatccac ttatcgactt tctggcgagc cgcattgcag
    25981 aatatgaaaa caataacgaa aagtttgcag agttcgacaa agccgttgca gcaatgcctg
    26041 ttggtgtggc gttacttcgc acgctgattg atcaacataa tctgacctat gcagatttga
    26101 agaacgaaat cggctctaaa tccctggtca gccagatttt atctggacaa cgttccctga
    26161 cgatatccca cattaaggca ctttcagccc gatttggcgt taagccggaa tggtttcttt
    26221 gactgatggc agacgctaaa tcgcctgatg cgctacgcct atcgggcttt gaaagggcgc
    26281 agattcttaa ttttgcgcgc ccttgtttgt cattcaggct gttgtcttta ctactcttaa
    26341 tttatcagta agctcaatca gatatgcttt tgctttttcg cgtgctcgcc cctcgaaaag
    26401 cattatccac tcatgtgtgt cgtcgattgt ttgtaaacgg ccctggagtt ttttgctgaa
    26461 agtcgacgtg agctgataac cgtcgagttg atcaatttgt ttactcgatg tgattttttt
    26521 cccggctgct ttttgaatga taagttcaac gagaccgata acattattat cgtaattatc
    26581 gtagagaaat tcttcatctc cctgaaatcc gaacaaatct tcatgctggc ttaagcgttg
    26641 aacaattgac tcggcatcaa aggtcgaatc atcttgcagt tgttcctcta cccaattaat
    26701 caaatccagc ccttcgtcgc tttcgagagc ctccattccc catgaaccca ttttgta
//
LOCUS       DAPQZD010000055        26238 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPQZD010000055 DAPQZD010000000
VERSION     DAPQZD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.055"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..165
                     /locus_tag="RO454_002267"
     CDS             <1..165
                     /locus_tag="RO454_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0934097.1"
                     /translation="YTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRI
                     GFNFSSIEDG"
     gene            542..799
                     /locus_tag="RO454_002268"
     CDS             542..799
                     /locus_tag="RO454_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HDY0934098.1"
                     /translation="MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRAQLDNR"
     gene            811..1362
                     /locus_tag="RO454_002269"
     CDS             811..1362
                     /locus_tag="RO454_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0934099.1"
                     /translation="MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDES
                     ARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
                     QPMNVTGHIQCAQALMKPEHWRE"
     gene            1414..2160
                     /locus_tag="RO454_002270"
     CDS             1414..2160
                     /locus_tag="RO454_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0934100.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPETRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQARRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGTVELLAQSLKPGGIMLIGEPYWRQLPATDEIAQACGVSS
                     TTDFLTLPGLVSAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNISPKRHVTYAREYFGWGVFALIAR"
     gene            2288..2548
                     /locus_tag="RO454_002271"
     CDS             2288..2548
                     /locus_tag="RO454_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934101.1"
                     /translation="MSQHEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            2586..2702
                     /locus_tag="RO454_002272"
                     /pseudo
     CDS             2586..2702
                     /locus_tag="RO454_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2947..4068
                     /locus_tag="RO454_002273"
     CDS             2947..4068
                     /locus_tag="RO454_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDY0934102.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            4065..4343
                     /locus_tag="RO454_002274"
     CDS             4065..4343
                     /locus_tag="RO454_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDY0934103.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            4355..5668
                     /locus_tag="RO454_002275"
     CDS             4355..5668
                     /locus_tag="RO454_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDY0934104.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            5681..6487
                     /gene="sgcQ"
                     /locus_tag="RO454_002276"
     CDS             5681..6487
                     /gene="sgcQ"
                     /locus_tag="RO454_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HDY0934105.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            6618..7049
                     /locus_tag="RO454_002277"
     CDS             6618..7049
                     /locus_tag="RO454_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDY0934106.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            7061..7693
                     /locus_tag="RO454_002278"
     CDS             7061..7693
                     /locus_tag="RO454_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HDY0934107.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            7710..8492
                     /locus_tag="RO454_002279"
     CDS             7710..8492
                     /locus_tag="RO454_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0934108.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            8795..9583
                     /locus_tag="RO454_002280"
     CDS             8795..9583
                     /locus_tag="RO454_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDY0934109.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKIELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            9588..10493
                     /locus_tag="RO454_002281"
     CDS             9588..10493
                     /locus_tag="RO454_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDY0934110.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            10504..12471
                     /gene="yjhG"
                     /locus_tag="RO454_002282"
     CDS             10504..12471
                     /gene="yjhG"
                     /locus_tag="RO454_002282"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HDY0934111.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQIKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            12578..13927
                     /locus_tag="RO454_002283"
     CDS             12578..13927
                     /locus_tag="RO454_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDY0934112.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            14274..15260
                     /locus_tag="RO454_002284"
     CDS             14274..15260
                     /locus_tag="RO454_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDY0934113.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(15301..16305)
                     /locus_tag="RO454_002285"
     CDS             complement(15301..16305)
                     /locus_tag="RO454_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HDY0934114.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVACVVSAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(16409..17050)
                     /locus_tag="RO454_002286"
     CDS             complement(16409..17050)
                     /locus_tag="RO454_002286"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDY0934115.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGIPEAGKIPSSEWLFHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(17081..18196)
                     /gene="mtnA"
                     /locus_tag="RO454_002287"
     CDS             complement(17081..18196)
                     /gene="mtnA"
                     /locus_tag="RO454_002287"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDY0934116.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQEMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(18206..19465)
                     /locus_tag="RO454_002288"
     CDS             complement(18206..19465)
                     /locus_tag="RO454_002288"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HDY0934117.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWPTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            20265..20519
                     /locus_tag="RO454_002289"
     CDS             20265..20519
                     /locus_tag="RO454_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDY0934118.1"
                     /translation="MKNGIDVSVIIPAYNAAETSVSLVTKILSEPHLAIELIIVDDGS
                     TDETGNVLRQIHDDRLILIEQANQGVYAARNAALALHQGE"
     gene            21623..23698
                     /locus_tag="RO454_002290"
     CDS             21623..23698
                     /locus_tag="RO454_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0934119.1"
                     /translation="MDLVDSVEAGKLTISELIDALAKDKNYSASKWDQRYREFTTLLQ
                     QTSTFSEPETDGLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYRSLRNRVFAAFSPENYTSTVDENAFSKAAEFLNQH
                     FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIA
                     STTFITSNEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            23691..24977
                     /locus_tag="RO454_002291"
     CDS             23691..24977
                     /locus_tag="RO454_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDY0934120.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
     gene            25087..>26238
                     /locus_tag="RO454_002292"
     CDS             25087..>26238
                     /locus_tag="RO454_002292"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     endonuclease"
                     /protein_id="HDY0934121.1"
                     /translation="MKMGKIAVTPNNDKAASHSKDENLATNTIKKNVKPKYGDAAFLE
                     YTRLIVNHPNYFGMPDPFGEKGEIQWEAPSNRASGKFKHTHQRRYEWWKNKARSIGID
                     PDTEKAWISKTAKLIHPLGVKPCKKCGKEMELSYSYPNEHFFSRVRKLNYIDETFELS
                     QNEHIVDLLTRLDDRFGERIYLDLPHLFSTKSITIPDISSNLEAWIEYLKEQYIPQES
                     RMLSPGAMANPPDRFDGFHSFNRCCRSIADKGRTKENLKSYVTDRRVFEYWVDGDWVA
                     ADRLMGQVRTNNIFINEECLNAGNGGLHPTPCQADHIGPISLGFSHRPQFQLLCKSCN
                     SAKNNRMYLSDIISLLEAENEGHTVISWFAEEVWNRLKHSVDDSEKALRL"
BASE COUNT         6878 a         6360 c         6347 g         6653 t
ORIGIN      
        1 tatactcgcc agggatatgc aattgaagca attaccgcca cgattgactg gataaaagag
       61 aacggttttt cactcatcaa agccggtgtg aatccggaaa ataccctttc aaaaaaatta
      121 cttatcagga ttggcttcaa tttttcgagc atcgaagatg ggtgagcata tttatgttct
      181 ggattaaaca aaaggagaat aatctcccga agggaaaagc cgatgaggcc atttcaggcg
      241 tacttcaaca tgcattcgtc agtgtcatct gccttttcca gtaaatgggt tggcattcaa
      301 tttccaaagt acatcacact ttttctactg aaacatatcc ctcctataac tacgtgttaa
      361 tattcgctcc gtaaatacct ttcaactcaa cccgaggctt tgatgctgga gaatgtaatc
      421 atccgataat cagttccccg accttttcag gccggactga ttatcaatgc gccgaaatcg
      481 aatgcggaca ccgcggtgtg tttgcacgtt ttgcacatga tgattaaaca ggttcttcag
      541 tatgaattta tcccgtcagg aacaacgtac gttacacgtt ctcgctaaag gtggacgtat
      601 tgcgcacgtc cgcgattctt caggccgcgt cacttccgtt gaatgctaca gccgcgaagg
      661 gctgttgctg accgactgca cgctcgccgt cttcaaaaaa ctcaaaacca aaaaacttat
      721 caagtccgtc aatggtcagc cctatcgaat caacactacc gggctgaata acgttcgcgc
      781 tcagctcgat aatcgctaag gagagtttcg atgacagcac accacttcac ttttcaaatc
      841 acagatgaaa gtgatgccag tgatatccgg gaagtcgaaa cccgcgcatt tggcttcagc
      901 aaagaagccg atttaacggc ctctttactt gaggatgaaa gcgcgcgccc tgcgttatcg
      961 ctgctggccc gacatgaagg caaagccgtc gggcatattt tatttacccg ggcaaccttc
     1021 aaaggggaaa tggattcacc gctgatgcat atcctcgctc ccctggcagt cataccggaa
     1081 taccagggga tgggcgtggg aggacggttg atacgcactg gcatcgagca cctgaggttg
     1141 atgggctgcc agacggtttt tgttctcggc cacgccacct attacccccg acatgggttc
     1201 gaaccctgtg ccggagacaa aggctatccg gcaccttatc ccatcccgga ggagcacaaa
     1261 gcctgctgga tgatgcaagc actcaccgct cagcccatga acgtgaccgg ccacatccag
     1321 tgcgcgcagg ccctgatgaa accggagcac tggcgtgaat aacaatcttg ttgtgccctt
     1381 tttaatgctt aacccaataa caggaattaa ctaatggata ttccacgtat ttttaccatc
     1441 agtgaaagcg aacaccgcat ccacaacccg ttcaccgaag agaagtacgc cacgctgggt
     1501 cgcgtgctgc gcatgaaacc agagacccga attctggacc tcggcagcgg ctcgggcgag
     1561 atgctctgca cctgggcccg ggatcatggc attacgggga ctggaatcga catgagttcg
     1621 ctcttcaccg cgcaggccag acgccgcgcg gaagaactcg gcgtcagcga acgcgtacat
     1681 tttattcata atgatgcggc gggttacgtc gccaatgaaa aatgtgacgt agcagcctgc
     1741 gttggcgcga cctggattgc cggaggcttt gccgggacag tcgaactgct ggcgcagagc
     1801 cttaaaccgg gcgggatcat gctcatcggt gaaccttact ggcgtcagct tccggcaacg
     1861 gacgagatag cccaggcctg tggcgtcagc tcgacgaccg attttctgac gctaccaggg
     1921 cttgtcagcg cgtttgacga cctcggctac gacgtggtgg aaatggtact ggcggaccag
     1981 gaaggctggg accgatatga ggccgcgaaa tggctgacca tgcgccgctg gctggaggcc
     2041 aatcctgacg atgacttcgc cgccgaagtc agggccgagc taaacatctc gccgaaacgc
     2101 cacgtaacgt acgcgcggga atactttggc tggggcgtgt tcgcactcat cgctaggtaa
     2161 caacaggttc ccggtatttc tgactggaga taccgggggc ttagcctttg ctttaatctg
     2221 atgcatcacc tgcacaatac atattcagta tgtttattaa tcaagatgtc accataagga
     2281 gcatcatatg tcacaacatg aacagttctg tcaggcttgc ggaatgccga tgtcggcacc
     2341 agatgcacac agtgccagtg atcaatattg tgcgtactgc agcgacagca acggcaatct
     2401 caaaccctgg gaagaagcgg tatcaggttt agccagcttc cttgattcct ggcaaaaagt
     2461 cggcccggag gaagcacata agcgtgcaaa acgttatctg acggcaatgc cagcctgggc
     2521 tcataaagcg gagagtgatt tgagctgatt atctatactc cctctgaatc tattcaggag
     2581 atccgatgtt ttcttacatt atgctgggaa ccaacgacct gccacgcgcc attacattct
     2641 aagatccgct gatggagatg ctggggtatc cacgtgcagg acgtaatgaa gagggagcgt
     2701 cctgcaacaa agtggtgcga taacagttac cgcgttttat tcattctcat tatttcaacc
     2761 ataatcactg gtctctctag cccgtaactt tcacaaacgt tgagcgcttt tattccactc
     2821 ggctcacata attttttatt tgttcagctt tgcgcgtttt cacgtgaacg ttctcacaat
     2881 tattcgacca gtctgcgcct ataaaacgct caacgagtga ttgttttacg gtctacggag
     2941 atagctatgt cattttctgt gcaggaaacc ctgttctcgc ttttgcagca caatgcgatt
     3001 tcaggacacg aaaacgctgt cgctgacgtc atgctgtgcg aattcaggcg tcaggcaaaa
     3061 gaggtctggc gagacaggct tgggaatgtc gtcgcgcgct acggtagtga taaacccgat
     3121 gcgctgcgac tgatgatttt tgcgcatatg gatgaagtcg gttttatggt gcgcaaaata
     3181 gagccttctg gatttttacg ctttgaacgc gtaggcggtc ctgcgcaggt cactatggct
     3241 ggatccatcg tcaccctcac cggggacaaa gggccagtca tggggtgtat cggcattaag
     3301 tcctaccact ttgccaaagg cgacgagcgc acgcagtcac cttctgtcga caaactgtgg
     3361 attgatattg gtgccaaaga caaagacgac gctatacgaa tgggcattca ggtcggtacg
     3421 cctgtcactc tgtataaccc gccgcaactc ctggcaaacg atctggtgtg cagtaaagca
     3481 ctagacgatc gtctgggctg tactgccctg ctcggtgtag cggatgctat cagtactacg
     3541 gagcttgata tcgccgttta tctggtggct tcggtacagg aagaatttaa tatccgcggc
     3601 attgttcccg tattacgccg tgtaaaacct gacctggcga ttggtattga tatcactcca
     3661 tcgtgtgaca cccctgattt acacgattat tccgaggtca ggattaatca gggcgttggg
     3721 atcacctgcc tgaactacca tggtcgggga acgctggccg gattaatcac gcctcctcgc
     3781 ctgatacgga tgttggaaca gacggctctt gaacacaaca ttccggtgca gcgagaagtg
     3841 gctcccggcg tgataacaga aaccggttat atccaggttg agcaggatgg tattccctgc
     3901 gccagtctct ctattccttg tcgctatacc cattctccgg cggaggttgc cagcctgcgt
     3961 gatttgactg attgcatccg cctattgacc gctctggcag gtatgtcagc agcacatttc
     4021 cccgttgagc ctgattcagg cactacacaa gaggcacatc cattatgaaa aagatccttg
     4081 tggcatgcgg taccggcatg tcgacttcaa ccatgattgc acacaagctg caagagttcc
     4141 tgactgaaca gggcatttca gccactaccg cccaatgctg tctgaatgag atccctctta
     4201 actgtaacgg tatggacctt attgtaacct ccatgcgaac caacagtgat tacggcatcc
     4261 ctacgcttaa tggcgccgcc ctgctcacag ggatcaacga cgacgcgtta aaacaacaaa
     4321 tcaaggctct gttaactcaa taaaaggagg cattatgttt gattacatcc tgtctctcgg
     4381 cggtaccgtt ttcgtcccta tcatcatgat cgtgattggc cttatcttcc gcatcccctg
     4441 gctgcaggcg attaaggcag gggtaacggt tgggattggt tttgtcggta tgggactggt
     4501 gatcgtcatg gcgatcgaca gcctcagtcc ccctatcaaa gtgatgattg agcgtttcgg
     4561 cctggccctg catgtctttg acgttggcgc aggccctgcc tccggcgttg gttatgcgac
     4621 agccattggc gccatgatta tcccagttat ttttcttctc aatgtcgcca tgctggtcac
     4681 ccgtctgacc aaaacgatga acgtcgatat ttacaattac tggcactacg ccattacggg
     4741 tacggtggtc cagcttatga ccggtagcct gatttatggt gtgctggggg caatctgcca
     4801 tgcggcgcta tcgttaaaaa tggccgactg gacggcaaaa cgggtacaga acatcgtcgg
     4861 gctggaaggt atttcgatac cgcaaggcta cggttccagt tcagtaccgc ttttcgttct
     4921 acttgatgct atctatgaaa aaatcccttt catgaaggga cgcaatatcg atgctcagga
     4981 gatccagaaa cgctacggca tggtcggcga tccggtgatt atcggcgtag tgctcggtct
     5041 gatttttggc ctggcggctg gtgaaggctt taaaggttgc gccagtctga tgattaccgt
     5101 tgcggcaatc atggtgctgt tcccgcgcat gatccgcctg attgttgagg gcctgatgcc
     5161 catctccgac ggtgcaagaa agttcttcca gaaatatttc aaaggacgtg aagtgtatat
     5221 cggtctggat actgctgtga cgctggggca tccaaccacc attgctgtcg gtctgctact
     5281 gatacccatt atgttaattc tggccagcat tttaccgggc aacaaagtgt tacccctcgc
     5341 agacctgccg gtagcaccgt tctttatctg tatggctacg gttatccacc gcggagacct
     5401 ggtcaggacg ttgataagcg gggtcatcgt gatgattacc gtcctgctga tcgccaccca
     5461 gttcgcgcct tacttcactg aaatggcgct caaaggcggt ttcagttttg ccggtgaaag
     5521 tgcgcaaatc tcagcgttgt ccgtgggcaa tatgttcggt tggtcgattt ctgaattgat
     5581 gtcgcttggg attattggcg ttgtcgtggc ggtcggtatt gtcgccagcg tcgtgctgtt
     5641 cttacgtaaa cgtgaattat cggaataagg agtactttca atgagttggc tgaaagaggt
     5701 tattgggacg gaaaaagcgg tcatcgccat gtgccatcta cgtgcattgc ccggcgaccc
     5761 gagttttgat gcgcagttgg ggatgaactg ggttatcgac aaagcctggg acgatctgat
     5821 ggcgctccaa aatggtgggg tggatgcagt catgttctcc aacgagttta gtcttcccta
     5881 tctcacgaag gtgcgtccgg aaaccactgc ggcaatggca cgaatcatcg ggcaattaat
     5941 gagcgatatc cgcatcccgt ttggcgtgaa tgttctgtgg gatccggtcg cctcattcga
     6001 cctggcgatg gctaccggcg ctaagtttat ccgcgagata tttaccggtg cctatgccag
     6061 cgactttggc gtctgggaca ctaacgtcgg tgagaccatc cgtcatcaac accggattgg
     6121 tgcaggcgaa gtgaaaacct tgttcaatat cgtcccagag gccgccgtgt atctgggcaa
     6181 tcgcgatatc tgttctattg ctaaatcgac agtatttaac aaccatcctg atgcgctctg
     6241 tgtttctggc ctgaccgccg ggacgcgtac agacagcgcg ctgttaaaac gggtcaaaga
     6301 aacggtgcct gacaccgtcg tgttggccaa cacgggcgtc tgcctggaaa acgtggagga
     6361 acaactcagt atcgccgatg gctgtgtgac cgcaaccacc tttaaaaagg acggcgtttt
     6421 tgccaatttc gttgaccagg cgcgagtcag tcagtttatg gagaaagtgc atcatatacg
     6481 ccgataagat agggataagc agagcgctta tccccggcaa agagcaataa cccttctgcg
     6541 atccctgccg ccttcgtcaa aagtatcgac ggggatcgct accagattaa cccgaatcgg
     6601 catggagtca acgacggatg attaacgata ttaaatgggt acaggcacaa cgtgaagcca
     6661 ccgactggcg tcaggccgtg gaaatcgcca cccgcccgct ggtagcctat ggtgccgcgc
     6721 agccatgcta cgttaatggg atcatcgaaa atacccttaa ctgggggcct tattatctga
     6781 tagccccagg aatcgccttg cctcacgccc gaccagagca aggcgcaaac tataatcagg
     6841 tcagtatcac cacgctacgc accccggtcg cctttggtaa cgaggagtgc gaccccgtct
     6901 ggctgcttct ctgcgtcagc gccacagatg ccaacgcaca tattttgacc attcagcgca
     6961 tcagccagtt tattgattcg ccacaacgct tagcggcagt gggaaatgcc agcacagacg
     7021 atgcactgtt tgcgctggta tcaggttaac ggaggcgctt atgatcctgc acccttctct
     7081 cgccagcgca aatcctctgc actatggtcg tgagctgacg gcattggaca atctggattt
     7141 tgggagcctg catctggata ttgaggacag cagcttcatt aacaacatta cgtttgggat
     7201 gaaaacggtg caggccgtcg ccagacagac gcctcatccc ttgtcctttc atttcatggt
     7261 tgcccggcca cagcgctggt ttaacgcgct ggctgaaatc agacccgcgt ggatcttcgt
     7321 tcatgcagaa acgctggact atccttcgga aacgttaact gagatccgcc ataccggcgc
     7381 acgagccgga ctggtattta atccggcaac cccgatcgac gcctggcgat atctggcgtc
     7441 tgagctggac ggagtgatgg tgatgaccag tgagcccgat gggcagggac agcggtttat
     7501 tccctccatg tgcgagaaaa tccaaaaagt acgcacggct tttcctcaga ccgagtgctg
     7561 ggccgacggt ggaattacgc tggcagctgc acagcaactg gcagcagccg gggcgcaaca
     7621 tatggtgatt gggcgcgccc tgttctcttc atctgactat cgcgcgacct tagctcaatt
     7681 cgccaccctg taagaggaga ttgtttctga tgagccaaca gagacctgac cgtatcaaac
     7741 agatgctgca ctacctgtgg caacatcgtc acctctccac acagcaggcc atggagcttt
     7801 ttggctatgc ggaagcgact gtccgtcgtg atttccagta tattgccaac cagtatccag
     7861 gcatgatccg cggtcacggt tgcctggact tcgacgacag tacagacgac aaagagtacg
     7921 tcttcgacgt gaaacgtaca ttgcaatctg tggcgaaaag agaaatagct gcccttgccc
     7981 gaacaatgat caaagatggc gactgctttt ttctcgactc cggctcaacc tgcctcgaac
     8041 tggcgaaatg cctggccgat gcgcgggtaa aggtcatctg caacgacatt aaaatcgcca
     8101 atgaactggg ctgttttccc catgtggaaa gttacattat cggaggactg attcgcccag
     8161 gttatttttc ggtaggagag agtctggcgc tggagatgat caatgccttt tccgtagaac
     8221 gtgcgtttat ctcctgcgat gcgctgtcgc tggagacggg gatcaccaat gccaccatgt
     8281 ttgaggtcgg ggtgaaaaca cggattattc aacgctcgcg tgaagttatt ctcatggccg
     8341 atcactcaaa atttgatgcc gttgagccgc atgctgtagc aacactatca tgtattaaaa
     8401 cgatcatcag tgacagcggg ttacctgaga ctattgcaca gcgttatcag cgagccggat
     8461 gtcagttgtt tttacctcat tcaataaagt gataagtaag ttcgttcgag agggatttca
     8521 agcaaaaata atcaatggca cccaatagaa aatattggcg atgcgctcga acgaataaag
     8581 aagccctatg cgcaatccac acgctacgat gttgcaacaa cacgccatct actttttatt
     8641 ctcattcact aaatgtggtt gttctggatt cattattcaa agtgtgtaca agatcacatt
     8701 taatcacttc attacagttc agcatgctga acaaagcata ttttccacta tgtaatgccg
     8761 ataccattta ttccatgagc aagaaggagc cattatggtt cgcaaagggt gtaattcatt
     8821 agtccgtgct gaaaaaatat taacccacat tgcatgggtt ggtatggcaa gttatatgga
     8881 gttattaaat aaattccagt accccaaaag cagcctgctt aatttattga atgtaatggt
     8941 ggagtgtggt ttcttaatta aaaacaaaaa cggctactat tcactgggaa taaaaaacta
     9001 cgaattgggt tgtcaggcgt tacaccgtca aaatatattt gaggtgacta agcggccaat
     9061 gcaggagttg tcgctgaaaa gtggactggt ttgtcatctt ggggcgatgg agagcatttc
     9121 ggctatctat ctcgacaaga tcgaaagtcc ggactctgta ccgacgagca aaagctggat
     9181 tggcaaaaaa attgagttgc atattaccgc gttgggtaag gcattattag cctggaagac
     9241 acgagaagaa ctggattatt ttttagaagc actcacgtta actccacata cgcgcaatac
     9301 atttaccgat aaaaaattgt ttctggaaga attacaaaaa acacgccttc ggggatgggc
     9361 catagacaac gaagaatcaa cctatggggc ggtatgttta agtatgccgg tattcaatat
     9421 gtataacaga gttaactatg caatctcact ttctggcgat ccggtagttt attcaggaaa
     9481 taagatagac agctatctgg aattgctccg gaaatgcgct gagcaaatat catatgggct
     9541 gggatacaga aacgaaaatg agcatttacg aaaaggaaac tgaggtaatg aaaaaattca
     9601 gcggcattat tccaccggta tccagcacgt ttcatcgtga cggaaccctt gataaaaagg
     9661 caatgcgcga agttgccgac ttcctgatta ataaaggggt cgacgggctg ttttatctgg
     9721 gtaccggtgg tgaatttagc caaatgaata cagcccagcg catggcgctc gccgaagaag
     9781 ctgtaaccat tgtcgacggg cgagtgccgg tattgattgg cgtcggttcc ccttccactg
     9841 acgaagcggt caaactggcg cagcatgcgc aagcctacgg cgctgatggt atcgtcgcca
     9901 tcaaccccta ctactggaaa gtcgcaccac gaaatcttga cgactattac cagcagatcg
     9961 cccgtagcgt caccctaccg gtgatcctgt acaactttcc ggatctgacg ggtcaggact
    10021 taaccccgga aaccgtgacg cgtctggctc tgcaaaacga gaatatcgtt ggcatcaaag
    10081 acaccatcga cagcgttggt cacttgcgta cgatgatcaa cacagttaag tcggtacgcc
    10141 cgtcgttttc ggtattctgc ggttacgatg atcatttgct gaatacgatg ctgctgggcg
    10201 gcgacggtgc gataaccgcc agcgctaact ttgctccgga actctccgtc ggcatctacc
    10261 gcgcctggcg tgaaggcgat ctggcgaccg ctgcgacgct gaataaaaaa ctactacaac
    10321 tgcccgctat ttacgccctc gaaacaccgt ttgtctcact gatcaaatac agcatgcagt
    10381 gtgtcgggct gcctgtagag acatattgct taccaccgat tcttgaagca tcagaagaag
    10441 caaaagataa agtccacgtg ctgcttaccg cgcagggcat tttaccagtc tgaggagaaa
    10501 ctcatgtctg ttcgcaatat ttttgctgac gagagccacg atatttacac cgtcagaacg
    10561 cacgccgatg gcccggacgg cgaactccca ttaaccgcag agatgcttat caaccgcccg
    10621 agcggggatc tgttcggtat gaccatgaat gccggaatgg gttggtctcc ggacgagctg
    10681 gatcgggacg gtattttact gctcagtaca ctcggtggct tacgcggcgc agacggtaaa
    10741 cccgtggcgc tggcgctgca ccaggggcat tacgaactgg acatccagat aaaagcggcg
    10801 gccgaggtta ttaaagccaa ccatgccctg ccctatgccg tgtacgtctc cgatccttgt
    10861 gacgggcgta ctcagggtac aacggggatg tttgattcgc taccataccg aaatgacgca
    10921 tcgatggtaa tgcgccgcct tattcgctct ctgcccgacg cgaaagcagt tattggtgtg
    10981 gcgagttgcg ataaggggct tccggccacc atgatggcac tcgccgcgca gcacaacatc
    11041 gcaaccgtgc tggtccccgg cggcgcgacg ctgcccgcaa aggatggaga agacaacggc
    11101 aaggtgcaaa ccattggcgc acgcttcgcc aatggcgaat tatctctaca ggacgcacgc
    11161 cgtgcgggct gtaaagcctg tgcctcttcc ggcggcggct gtcaattttt gggcactgcc
    11221 gggacatctc aggtggtggc cgaaggattg gggctggcaa tcccacattc agcccttgcc
    11281 ccttccggtg agcctgtgtg gcgggagatc gccagagctt ccgcgcgagc tgcgctgaac
    11341 ctgagtcaaa aaggcatcac cacccgggaa attctcaccg ataaagcgat agagaatgcg
    11401 atgacggtcc atgccgcgtt cggtggttca acaaacctgc tgttacacat cccggcaatt
    11461 gctcaccagg caggttgcca tatcccgacc gttgatgact ggatccgcat caacaagcgc
    11521 gtgccccgac tggtgagcgt actgcctaat ggcccggttt atcatccaac ggtcaatgcc
    11581 tttatggcag gtggtgtgcc ggaagtcatg ttgcatctgc gcagcctcgg attattgcat
    11641 gaagacgtta tgacggttac cggcagcacg ctgaaagaaa acctcgactg gtgggagcac
    11701 tccgaacggc gtcagcggtt caagcaactc ctgctcgatc aggaacaaat caacgctgac
    11761 gaagtgatca tgtctccgca gcaagcaaaa gcgcgcggat taacctcaac tatcaccttc
    11821 ccggtgggta atattgcgcc agaaggttcg gtgatcaaat ccaccgccat tgacccctcg
    11881 atgattgatg agcaaggtat ctattaccat aaaggtgtgg cgaaggttta tctgtccgag
    11941 aaaagtgcga tttacgatat caaacatgac aagatcaagg cgggcgatat tctggtcatt
    12001 attggcgttg gaccttcagg tacagggatg gaagaaacct accaggttac cagtgccctg
    12061 aagcatctgt catacggtaa gcatgtttcg ttaatcaccg atgcacgttt ctcgggcgtt
    12121 tctactggcg cgtgcatcgg ccatgtgggg ccagaagcgc tggccggagg ccccatcggt
    12181 aaattacgca ccggggatgt aattgaaatt aaaattgatt gtcgcgagct tcacggcgaa
    12241 gtcaatttcc tcggaacccg tagcgatgaa caattacctt cacaggagga ggcaactgca
    12301 atattaaatg ccagacccag ccatcaggat ttacttcccg atcctgaatt gccagatgat
    12361 acccagctat gggcaatgct tcaggccgtg agtggcggga catggaccgg ttgtatttat
    12421 gatgtaaaca aaattggcgc ggctttgcgc gattttatga ataaaaactg aaagcataaa
    12481 taaaaacatc cagacacctg aattatccga ctctgcatca ttcgttattt gctcagtcgg
    12541 ataatgatgg gtcgctaccc tgttatgaga ggacgttatg ccactaatta tcgttgtggc
    12601 agggattgct ttactcctgc ttttaaccat aaaaattaag cttaatacgt ttgtttcgtt
    12661 aattattgtc tcgattgctg tcgccatcgc cagtggtatg gatttgagta aagtcgtcac
    12721 ctcagtcgaa tccgggcttg gcggtacgct gggccatatt ggtttgatat ttggcttcgg
    12781 tgttatgctc ggtcgcttgc tggccgatgc gggtggtgcg cagcgaatag cgctgaccat
    12841 gctgaattat ttcgggaaaa ataaacttga ctgggcagtg gtctgttcgg cgtttattgt
    12901 cggtatcgca ctttttttcg aagtcggttt aattcttctg gtacctattt tattcgctat
    12961 tgcccgtgaa gcaaaaatat caccgatgtt tatgtgtgtg ccgatgcttt ctggtttgct
    13021 ggtcgcacac ggttttttgc cgccgcaccc cggcccgact gttatcgcca gggaatatgg
    13081 cgctgatgta ggattggtac tgatatacgg tatcattgtc ggcattccaa cctttattct
    13141 ttgcggcccg gtattgaata aattctgcca gcgcattatt ccggacgcct ttaaaaaaga
    13201 ggggaatata gcctctctcg gcgcgacccg ccgattcagc gaaagcgaaa tgccaggctt
    13261 tgggatcagt tttctcacgg caatgctgcc ggtgatcctg atggcggttg tcaccatcat
    13321 ccagatgact catgccaaaa gtgccgcgga ttctggctta ttttacaacg tgatactgtt
    13381 cttaggtaat tcgacgatcg ccatgctgat ttcactgctg ttcgcgatct ataccatggg
    13441 cctggggcgc gggaaaacaa tccctgacct gatggattct tgcggaaaag ctatcgcggg
    13501 tattgccggt ctgctgctga ttatcggtgg tggtggtgca ttcaagcaag ttctcatcga
    13561 ctctggcgtt ggtcagtata tttccaccct ggtttcgggt atggatatta acccgatctt
    13621 gatggcctgg ggcgtggccg cattcctgcg tatttgtctg ggttcagcca ccgtcgcggc
    13681 gatctctacc gccggtctgg tcatcccatt gttggcagta catcccaata ccaacctggc
    13741 cttgattacg ctggctaccg gtgccggatc ctgtatttgt tctcacgtta acgatgccag
    13801 tttctggatg attaaagact tttttggcct gaccaccaaa gaaacgctgt tatcctggac
    13861 gttgatgtcc acactcttat ccatcagtgg gctgattttt attctgctcg ccagcttagt
    13921 tttataatcg ggttcttgcc agtgagccaa cctgttgttc actgtccccc ataatttagt
    13981 gagtgacggc aaccgtctgc acggtgacga atcgctggcg atcaaaaagg ggcgatattg
    14041 aaccgtcgat tatgcttgat gggtgtactc tcagtaatac tgtaaagccg gagcatgtct
    14101 ccgactttac agtatggggg aacagcaggc gatcgctcaa atggtatgct ggtttatttc
    14161 atgtaaaaga ataaaatatc tgtataaaat catgccactg gcaatctgca tcaactctcc
    14221 gggtaaacag ggttaagatg agcatttccc cggtttaata caggtataca aaagtggata
    14281 gagatcaaac aaacagcagc ctgttcaatg atgatcccgt acttcatgca acatggctgt
    14341 actatcagga aggcaaaagc cagactgaag ttgcagctat catgggggtg tcccgcgtca
    14401 cggttgtcaa atacctgcaa acagcccggg aaaatggact ggttcatatc aacctggatg
    14461 tgaatgtctt tggctctatc gacgctgctt tgcaaattcg cgataaattc aatctccaga
    14521 gagtgatcat cgtgccggat ggcgaacatg ccggaaaacg tgatgataca aagctgatgc
    14581 ggactcgcct ttcacgtgcc ggtggcatgt atctgaatca ggtgattgag aatggtgatg
    14641 tgctgggggt tgcctgggga agaacaatcc atcagatgag caaaaccatg acgccgaagt
    14701 catgtaaaaa tgtcaccgtt atacagatgc tcggttccat gccatctcag ccagacctga
    14761 cgatcattga atcctcatcg caaatcgcct acaagctttc aggccgtgtc gcctcgctgc
    14821 acgtgcccgc cgtggtatca agcgccagac tggccatgga gttacaggca gagcctatta
    14881 ttcgatcgaa ttttgacgtt ctgactcgct gtacgaaagc cttttttgtc gtgggtaacg
    14941 cgcttgatga aaatcccctg attcgggtgg gcgtgctgaa taagaaggaa atgcagacgt
    15001 atcgggattt aggcgcagta ggtgtcattt gtggtcgctt ctatgacaag gaagggatgc
    15061 ccgtcgtggc tgatgttgac cagcgtattc tgggaatcag tctggctcag ctaaggcaga
    15121 ttgaacggaa gatcttcctt gctggtgggg aaagaggcta tgacgcaacg ttgggtgccc
    15181 tgctgggagg atatgtcacg gacctgattg ttgatgaagg aacggcagag tttttgctgg
    15241 cgtgtgaatt accacactaa aagaatgaac aactgcccca tgtggatatg gggcaggatt
    15301 ttattgatta gcggcgataa taagaacaat acccacaatg tatgaaataa acatggtcag
    15361 cagcagttta ttggttccgg ctggcgcact ggcaaattct ctccagatga agactcccca
    15421 gatcacggca atcattgtgg ccccctgtcc cagaccgtag gaaatggcat accccgcctg
    15481 accagacgca atcagactga atgccagacc gacacaccaa atcataccgc ccagccagcc
    15541 gcaaacatga tcccgtaagc tcccggaaaa atacattttc ccgttgaccg gttctcctga
    15601 aataggtttt ttcatgacca gcgtgttaag gacaaagttc gacaagaaca gccccacggc
    15661 aaacagaaca agtgctgagt aaggcgtcat cagaccgctg gcaggctggc tgaaattgtc
    15721 agacatggag tccgccaaga agcggaaaaa ccatcccatg atgatacccg ccagaatggc
    15781 agtaatgaga cctttattac ggcgagattt atccgcctcc tgcgtgacac gaccataggc
    15841 tatcgcggta aaaataatgg cgctcacaac gcaggcgaca ccgaggaaca gaatgagcgg
    15901 atcgccctgc ggattgccga tataggtggt gatcacaccc agagccagtg ccagccccac
    15961 acccacagga aaagctaccg ccataccggc aagattaata gaagcgacca gaagaatatt
    16021 ggaaatattg aagataatcc ctgccagtat tgccgacatc agactactgc tgctcgcctg
    16081 ctgaatatca ggtataaaac tgcgaccggc ttcgcccata gaacccagcg tgaaggcaaa
    16141 taacaaagaa cacagcaata atccaattga ataatcccaa tagaatagag ggaactccca
    16201 ttttttattg ctgacaagtt tagttgtatt cgcccatgaa ccccagcaaa tcatggtaat
    16261 aaaacacatt attatggcga cagcatacga ttccacgata aacataaata actccttgtg
    16321 gtagttataa tcaatcgttg tggacagagg gaatagcagc cattctgcga atggctgtca
    16381 tatgttatta attaaaataa agctgtaatt acgctttcaa accatacgtt ttgaattttt
    16441 caaggacgat ggtcatttcg gcttcagaca gcacgggaac atcgtgatgt aatccgccac
    16501 atttgatgta taaatcagcc agcgtctcgg tttcacccgc cagccacaac gtcttttcca
    16561 gcgtgacgtc catggccagc ataccgtggt gctgcatcag caaggactta ctttctctga
    16621 ttcctttaga tacaccgtcg gccagttcag gactgccaaa ggtataatag ggaatacagg
    16681 gaatatgatc cgtccccgat accgccacca tataatgaat ggccggaatt gaatgattat
    16741 gtattgccac cgccgtggaa ttcaccgcat gcgtatgtat gacagcattc aattctggtc
    16801 tggctttata acacgccaga tgaaataacc attcacttga tggtatttta cccgcttcag
    16861 gtattccctt atcgtctaca aagacaatca tgtccggcgt catctttgaa taagcgatcc
    16921 cactcggcgt aatcaacatg ccaccggtat acctggcact cacattacct gatgttcctt
    16981 gattaagacc tgacgcattc atttcccgac aagtggaaat gattttttct gctaacttaa
    17041 tccgttccat tcctgatcct taatagcaat ggccgccgtt ttaatgctgt tccccctgga
    17101 gtgctttact cttcaggtca gcaaacatat ccgccagcgc tgacttactg gcagcacaga
    17161 ctccccgctc agtaatgaag ccggtaatgt atcgggcagg cgtgacgtcg aaagcgtagt
    17221 tcccacaacg ggttccttca ggggcggtat tgacccagct caattctccc tgagggttta
    17281 tcccatagac atgggactgc tcttttccgt cacgttgctc aatcggaatg ctttttccgt
    17341 cctcgatagt ccagtcaata gtgggagaag gtaacgcgac gtagaaggga acatggttat
    17401 catgggccgc cagcgcctta agataggtac caattttatt acagacatcc cctctggccg
    17461 tggttcgatc ggtcccaaca atgcagagat caacgtcacc gtgttgcatc aggtggccac
    17521 ccgcgttgtc ggcgatcagg gtgtgaggaa taccgtgtga gcccagttca aatgccgtga
    17581 gtccgccctg attacgtggc cgcgtttcat ccacccagac gtgaacaggg attccgtttt
    17641 catgcgcttt gtagatggga gaaagcgcgg ttccccagtc tacagtcgcc agccagcctg
    17701 cgttacaatg tgtcaggata ttgaccacac tcccggccgg ttttttctgg gctatttcgc
    17761 ggatgacttc tgtaccatgt aatccaattt gttcacacaa cgcgacatct tcctcggcaa
    17821 tttctgccgc cagcgcccag gccacatcct tgcgctctga tacacagaga tccttcagtg
    17881 tgtctatcat cctgtcgagg gcccatttga gattaattgc cgttggtcgg gttttgataa
    17941 gaaggtcata gtaacgctgc agacccatgt cactggcatc gtgattcatt cccagcgcga
    18001 tgccataggc tgcaacgaca ccaataagcg gagcgccacg aacccacatc tcctgaatag
    18061 ccgtagcggc catttccgca gaggtaagcg ccaccacctc gaacttaaaa ggcaacttag
    18121 tctgatcgat gatttctacc gcttttccat ccccggaaac ccagaccgta cgatagtgtt
    18181 tacctttaat attcataatg acctcttact taaccttcgc agactggatg acggaatcca
    18241 tatttttaaa ctggcgggca ttgacgataa gatcgcgcgc catggtcaat gcacgtcgtt
    18301 cacatcctgc gcgaagctct gtattttcaa tctgtttcag ctcggcaacg ccagcaaaac
    18361 caatgatccg gcgattcatt tccatgccgg cattcaccag ggaatcttca agcagcgtgg
    18421 caaaaaactg atcctgtgcg tcctcaagag cggccctgtt ctgttgatac atttctgtcg
    18481 gccacgcgtc gccctgtgtt ttgttgtccc agagctggcg gaactcccgg gtgaaaacgg
    18541 accaggtttg gacaatctgc tgaagcagcc attcctgata gtcagcgcga cgttgctcat
    18601 tcgcatccca cccagggcgt gagaagtacg ccagcaggag gttgccaata tagttgccga
    18661 tatcaaacgc cattggcccc atgaaaccga actccggatc aatcactttg gtatccgtgt
    18721 cggtcaccat gattgagcct gaatgaaggt cgccatgtaa caatgcctgc gcttctgtca
    18781 taaatttgta cttataacgc atggcaacct ggatcatctc tacatcagcc caggccttat
    18841 ggacggcatc gtccagctca ggagaagtcc agttattccg ttcagcgttg taatagggct
    18901 ctgtgaagat cagatcttcc gtaattttgc acagctcatg attcaacgcg aactcggcag
    18961 taagcgcttt tttctgttct gctgacatgc caatgtcaga cgtattgaag agagtctgtg
    19021 ctaaaaatct gccgatatct tcagctagtt tagggaattt tttaccgtta atcagttcct
    19081 tacgcagaat cacgtgagga gtcagatact ccatggcaaa cagcgccatt tccgggtcgt
    19141 aaaaatagac ctgcggaatc agtgtgtgac ccgcgtactt ggcttcctga cataagacgt
    19201 tgtactcaaa gcccgcacgg ctcagagata aaggccatcc ttcgccccct gcacgaagcc
    19261 agggcagagc ttgttttaca atgatggttt tatgtgtccc ctcaacgata aacaccatgt
    19321 tcaggtttcc gtcccccact tctgaaactt tccactcttc aggtaaccct cccggttcgc
    19381 atgaaggggt cagtctggac gagagataac ccggcagcgt gtcacatgtc agcggcttgt
    19441 aacctgatgg aattgaatcc gtcataacag ttctccttgt ctacacagaa tgataatcaa
    19501 cttgtcatgt gacaactata cattatcaaa tgtaaaatta tttgtgacac atgtagattt
    19561 tttgaactgt ataatattaa aaatgatgtc gtatggagtc atgatcttta caggaatcag
    19621 cagaggaaaa catctattat taccatgtaa ttcatgttgt tatgtcgcaa caatagtact
    19681 gcacggggaa ttattacggg caaacttagg cgaaaggaag tactgaagtg acagaataaa
    19741 tcacactttt tgtacatatg tacttttcta ttgtcttttg ttgtggaaac aaatagtgtt
    19801 tgtcatgccg atgctaatag acagatagat tcgagggcca acagccccat catcatgatg
    19861 gagcaacaga ggtgagtcta acgtttccgc acttcttatc gatcggcact aacgatcaat
    19921 atcgataaac tgatctatca aaacgattat ctttcttgca acctgcctgg ttatccttct
    19981 cttacattgc tcgcagaaaa gcccttttat tacggttagt tatctagctg agacttgcta
    20041 cgcccatttt ccatgtaccc caaatcacga ggtcacaaag gcgcggaaag atgaagagat
    20101 gtgcacgttg caacaaaaaa agaagcgtcc ttgaacagag gctctcactc gataaagaat
    20161 tctgcgacga gtgcttattc gatatcgatg aggaaatcag ggaaagaagg attatcacga
    20221 tcagacgcct caaagatgac aacgaaagaa aatcgtagct caatatgaaa aatggaatcg
    20281 atgtcagcgt cattatccct gcttacaacg ccgctgaaac cagcgttagt ttggttacta
    20341 aaatcctcag tgaaccccac ttggctattg aacttatcat tgtcgatgac ggttctactg
    20401 atgaaactgg caacgttctt cgtcaaatcc acgatgatcg gctcattctc attgagcagg
    20461 ccaatcaagg tgtctatgcc gcccgaaatg ctgctctagc cttgcaccaa ggagaatagg
    20521 ttatattcct tgatgcggac gacgatatag cagatgggtt catagaaaaa agatggcagg
    20581 cagttagtac gtcgcaagcc gacgtcatga tctttaatgg ctggcattcc tgtgctgacc
    20641 aacatcgaag agcggttcac aaaaagcagc cctatggtac gcctctgtca ggttattaat
    20701 ggattagaca ctgtgttaca caccgcgaat ggccgcatta tctgtggctg cagatggtca
    20761 ggccatccta tgtcaggcag cattcactgc gcttccagtc cggcaggagc cataaagaca
    20821 ttttttaaac ggttcatctt gcagcgagca atggttgatt ctgtacattg gatataaagg
    20881 actatatcta tatcaacaat aaggcctcta tcacacatcg gccggattac tacgttgttc
    20941 gtgctataaa ctatgttgaa gtgatcgcga aaatcatcca agtagcggaa caagaacaaa
    21001 acaaaaaata aaggtatttt tgtaccggca tgcgctggta gaggcacgtc attttttggg
    21061 actctaccgc aatcgcgttg gggataaatc gggcatgaag gactgcttca aagcgaacat
    21121 attcgcagcc catttattca gaggtatcag cagttttagc gatgtgtttt tctttattaa
    21181 gctgctgggc aagctattct gataagttgt gcgttgctac acgacaccag cgcacagctt
    21241 aatttcacgc tttctgacta aattgttcag gccttttcta taggtgcaaa cacaactccc
    21301 cgtactttcc tcttccaact caaaatcatc gcaccttgct gacccaagaa gggagcatac
    21361 cctgcactag tatccgcaca gctaatactc tattgatacc aacaacgctt cagcacaaca
    21421 aagtaactcg ataatcacaa ctcaaagtga agatgccaaa aactcgtttg agagcaataa
    21481 attcaataac atgaaaaaaa tgtattttgc attgaaaatc ttacttattg cgcttggcta
    21541 caattgcatg cttggtaata tagattataa aacaccagac tagctgcctg gtaagtaatg
    21601 taatagcaga ggaaatatca cgatggatct tgtagatagt gtcgaagcag gtaagctgac
    21661 gatcagtgaa ttgattgatg ccctggcgaa agataaaaat tactcagctt ccaagtggga
    21721 tcagcgatac cgtgaattta cgaccttgct acaacaaacc tcaacttttt ctgaacctga
    21781 aacggatggt ttagtcaaga ggctctggta tgaacgcgat aacggtattg ccagtattcg
    21841 ccagggcgtt ccatccttag cagaatatca gcaaagcctg ccactgctta gagaactaac
    21901 tgaacgaatt cggcaacagc cggatgaaga aacttaccaa tatgttggca atgcactaca
    21961 acaagctaaa gaaaacggac ttctcaagcg tatgtatagg agtttgagaa atcgcgtctt
    22021 tgccgcgttc tcgccagaaa actacaccag tactgtggat gagaatgctt ttagtaaagc
    22081 agcagaattc ctaaatcagc acttccatct cggtctggca ctgaccggaa attggttaca
    22141 gaaaaactat gaattgaaac aagccataca cgcccaatct cctgacacag atccttatta
    22201 cgtgaatatg gccatctggc atctctatga attgctccgt gaacgcgata atgaacaaaa
    22261 gcaggagaaa atagcgagca ctacattcat aaccagcaat gagcccatcg agaacaagat
    22321 catcctacat tcaccaacta acgtgatctt ctttggcccc cctggcactg gcaaaacctt
    22381 caggttgcag caaaaaatga aagagtacac ttctcatgct gttccagctg atcgtgatgc
    22441 ctggctggat tctcgccttg aatcgttgaa ttggatgcag gttataacgc tggtgctgct
    22501 cgatcttggg aaacgagcca aagttcgcca aattattgaa catatgtggt ttcaacgtaa
    22561 ggcattatta aacggtcgta atggcaacct atcgaataca gcctgggcag ctttgcaatc
    22621 ctatacagtt cccgagtcgt taaccgttga ttataagaat cggcgtgagc ctgccgtatt
    22681 taacaaaaca gataacagcg aatggttgct agttgattca cagctcgagc aagtggagga
    22741 tttggtagag ctctacgccg aacttaaacg aggccctaaa tctgccgaag ccatccagcg
    22801 ttttgcggtg gttacgttcc accaatctta cggctatgaa gaatttattg aaggtatacg
    22861 cgctcgctct gacgagagtg gcaatatctc ttatcccatt gagccgggta tctttatgcg
    22921 cctttcccaa cgtgccaatg ccgatccagg acatcgctac gccattttca ttgatgagat
    22981 caatcgcggt aacatatcca agatctttgg tgaactaatc tcactcattg aagtagacaa
    23041 gcgtgcaggc atgcccaatg caatgagcct acaactggct tatagcggtg atcacttcag
    23101 cgtacccgcc aatgtcgata tcatcggagc catgaataca gcggaccgtt ctttagctct
    23161 gatggacacg gctttgcgcc gtcgctttga ctttgtcgaa atgatgcccg atctctcttt
    23221 actgagtgga gctaaggtga aaggcataga gctcgagtcg ttgttagaga aactcaatag
    23281 ccgcatcgag gctctttacg atcgtgaaca tacgctgggg catgcgttct ttatgccggt
    23341 aaaaaatgca ctcgatgccg gtgatgaaga agctgcgttt aaacaattga agatcgcatt
    23401 ccagaaaaag atcattccgc ttttacagga atactttttc gatgactgga acaagatccg
    23461 gttggtgctg gcagacaatc aaaagcaaga cgacaacctg caattcgtga ttgagaaaac
    23521 cgacgatctc gatacgcttt ttggtaacaa ccatggttta cgacaccatg atcagcaatc
    23581 aacagcttac gaactcaaag atttcgatca agagatctgg aatattccac aggcttatcg
    23641 ttcaatttat cagccccagc agactcccct tgatgagcag gctgtaaatc atgggtgaag
    23701 ttatctccgt ttttgaatat gacctattgg ggagtgacaa agcggcttca gttggcgcaa
    23761 aactggtgcc tccactggtc tttaattatt tagaagcgct tagcttggca agtaatcaag
    23821 ggagccagtt cctcaaactc acctctcgct caggcttcaa attgcttcag gttcaaaact
    23881 acgcggggat gctttcaaca cctcacggtt tccagcttga gatactgccc aaggttggta
    23941 aaaacctcac agcggctaat gcacgtcaaa cattactgac gatgctaagc catttgcctg
    24001 ggtttcgaca tattgaaact cagcaagcca ctcttcaggc tcagcgtatg cctttgcttg
    24061 agatttttat tcaccagttt ttgcatagcg ttagccaatt acttaaacaa ggtttacgct
    24121 ccgactatgt gagcgagcaa ggtaatctgg cttttatgaa gggcaagctg atgctttctg
    24181 cacagctgcg acataacgcg gtgaatcgcc ataagttttg tgtcgattat gatgactaca
    24241 tgcctgattg tgcagccaat cggctcttac actccgcgct agataagctt cttagtctaa
    24301 agctgtcatc agagaatcaa cgctggcttt acgaactgcg ctttgcgttt gacggtattc
    24361 cacttagccg ggatattgaa cgtgatataa acaacttacg cctcgagcgt ggtatggctc
    24421 attacaacga gccaatggcg tgggcgcaat tgatcctgag aggaatgagt ccgagtgctt
    24481 tgcagggaaa caccaaagcg atatcacttt tgttccctat ggaagcggta tttgaatcct
    24541 ttgtggcaca gaccctaccc tacgaattac cttctcatct caaagtaata ccacaagcgg
    24601 ctacttattc tcttgtgaag cacgggtcta acgattgctt taaacttcgg ccggatttgt
    24661 tgattcagtc acgtcgaccg gtacaaacca aattggtgat ggacacaaaa tggaagctaa
    24721 taaatagcaa ctcccaaaaa acaacacagt atgggctagc gcaagccgac ttctatcaaa
    24781 tgtttgccta cggtcaaaaa tacctaaatg ggattggcga aatgtactta atctatcctg
    24841 cgcatgatca atttaaccaa ccgatacagc agcattttgc tttctctaaa acattaaaac
    24901 tatgggttgt tccgtaccag ataacagcaa accgcgggga gaggatgatg tgggtgaagg
    24961 atgtgtccag tatataaatt tctagccaag ctggcgatga accagtcctc taaaaaatgg
    25021 gtataatcat agtttattta ctaattaaat taatataaag taattacttt attatatgga
    25081 gaatttatga aaatgggaaa aattgcagtt actcctaata atgataaagc agccagccac
    25141 tctaaagatg aaaatttagc cacaaataca attaagaaaa acgtaaaacc taaatatggc
    25201 gatgcggcat ttttagagta cactcgacta atagttaacc acccgaatta ttttggaatg
    25261 ccagatccct ttggggaaaa aggtgagatt caatgggaag ctccatcaaa tagggcatcg
    25321 gggaaattta aacacactca tcaacggcgc tatgaatggt ggaaaaataa agccagaagt
    25381 attgggatag accctgacac tgaaaaagca tggattagta aaacagctaa actaattcat
    25441 ccacttggag ttaaaccatg caagaaatgc ggtaaagaaa tggaattatc ttactcctac
    25501 ccaaatgagc atttcttttc tcgggttagg aaacttaatt atatagatga gacttttgag
    25561 ctatctcaaa atgaacacat agttgatttg ttgactcgtt tagatgatag atttggtgaa
    25621 agaatatatt tagatttacc acatttattt tctacaaaat ccatcacgat tccagatatt
    25681 tcaagcaatc tcgaagcatg gattgagtat ctaaaagaac aatacatacc tcaagaatca
    25741 cgtatgctta gccctggagc catggcaaat cctccagatc gttttgatgg atttcattca
    25801 tttaaccgct gttgtcgctc tattgctgat aaaggaagga ccaaagaaaa tcttaaatct
    25861 tatgttactg acagaagagt tttcgaatat tgggtggatg gtgattgggt agctgcagac
    25921 aggctaatgg gccaagttag aacaaataat atatttatta acgaggaatg tttgaatgcc
    25981 ggaaatggtg gtttacatcc aactccttgc caagcagatc acattggccc aatctcacta
    26041 gggttttccc atcgcccaca atttcaacta ctttgcaaat catgtaatag tgccaaaaac
    26101 aatcgtatgt atttaagtga tataatctct ttattagaag ctgagaatga aggtcatacg
    26161 gttatttcat ggttcgcaga agaagtatgg aatcgactaa aacactctgt agatgatagt
    26221 gaaaaagcct taagacta
//
LOCUS       DAPQZD010000056        25800 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPQZD010000056 DAPQZD010000000
VERSION     DAPQZD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.056"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(101..748)
                     /gene="ychE"
                     /locus_tag="RO454_002293"
     CDS             complement(101..748)
                     /gene="ychE"
                     /locus_tag="RO454_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDY0934122.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1225..3900
                     /gene="adhE"
                     /locus_tag="RO454_002294"
                     /gene_synonym="adhC"
     CDS             1225..3900
                     /gene="adhE"
                     /locus_tag="RO454_002294"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDY0934123.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4025..5072
                     /locus_tag="RO454_002295"
                     /pseudo
     CDS             4025..5072
                     /locus_tag="RO454_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5358..5975)
                     /gene="tdk"
                     /locus_tag="RO454_002296"
     CDS             complement(5358..5975)
                     /gene="tdk"
                     /locus_tag="RO454_002296"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDY0934124.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQAYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            6579..6992
                     /gene="hns"
                     /locus_tag="RO454_002297"
     CDS             6579..6992
                     /gene="hns"
                     /locus_tag="RO454_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDY0934125.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7137..8045)
                     /gene="galU"
                     /locus_tag="RO454_002298"
     CDS             complement(7137..8045)
                     /gene="galU"
                     /locus_tag="RO454_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDY0934126.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8247..9260)
                     /gene="rssB"
                     /locus_tag="RO454_002299"
     CDS             complement(8247..9260)
                     /gene="rssB"
                     /locus_tag="RO454_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDY0934127.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9352..10257)
                     /gene="rssA"
                     /locus_tag="RO454_002300"
     CDS             complement(9352..10257)
                     /gene="rssA"
                     /locus_tag="RO454_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDY0934128.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10370..10828
                     /locus_tag="RO454_002301"
     CDS             10370..10828
                     /locus_tag="RO454_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDY0934129.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10878..11720
                     /gene="purU"
                     /locus_tag="RO454_002302"
     CDS             10878..11720
                     /gene="purU"
                     /locus_tag="RO454_002302"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDY0934130.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11880..11964
                     /locus_tag="RO454_002303"
     tRNA            11880..11964
                     /locus_tag="RO454_002303"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11914..11916,aa:Tyr,seq:gta)
     gene            complement(11981..13072)
                     /locus_tag="RO454_002304"
     CDS             complement(11981..13072)
                     /locus_tag="RO454_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HDY0934131.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(13059..14183)
                     /locus_tag="RO454_002305"
     CDS             complement(13059..14183)
                     /locus_tag="RO454_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HDY0934132.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(14176..14778)
                     /locus_tag="RO454_002306"
     CDS             complement(14176..14778)
                     /locus_tag="RO454_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HDY0934133.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(14775..16061)
                     /locus_tag="RO454_002307"
     CDS             complement(14775..16061)
                     /locus_tag="RO454_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HDY0934134.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(16058..16864)
                     /locus_tag="RO454_002308"
     CDS             complement(16058..16864)
                     /locus_tag="RO454_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDY0934135.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(16842..17858)
                     /locus_tag="RO454_002309"
     CDS             complement(16842..17858)
                     /locus_tag="RO454_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDY0934136.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(17855..18862)
                     /locus_tag="RO454_002310"
     CDS             complement(17855..18862)
                     /locus_tag="RO454_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDY0934137.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(18990..19667)
                     /gene="narI"
                     /locus_tag="RO454_002311"
     CDS             complement(18990..19667)
                     /gene="narI"
                     /locus_tag="RO454_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDY0934138.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(19667..20377)
                     /gene="narJ"
                     /locus_tag="RO454_002312"
     CDS             complement(19667..20377)
                     /gene="narJ"
                     /locus_tag="RO454_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDY0934139.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(20374..21912)
                     /gene="narH"
                     /locus_tag="RO454_002313"
     CDS             complement(20374..21912)
                     /gene="narH"
                     /locus_tag="RO454_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDY0934140.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(21909..25652)
                     /locus_tag="RO454_002314"
     CDS             complement(21909..25652)
                     /locus_tag="RO454_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDY0934141.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         6632 a         6850 c         6213 g         6105 t
ORIGIN      
        1 tttttaccta ttccttttgc ttttgataac tgcgcaggaa caaagtaaaa ataatgacga
       61 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
      121 aaatcccctt aataccagta acgataaatt caatccccaa cgccatcagc aatagcccca
      181 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac tcgtaccagc cacggtgcca
      241 tgcgaaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
      301 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
      361 ccgccatcaa cggcaacgcc aatggcacca caccaatgct ttcacgcacc gcggtttctg
      421 atttttcttg cttgttctgt ttatcctcac caagcttgcc gctgatcatc gacatcgcta
      481 ttgtcaccac caggatgcca ccggcgatac ggaatgaatc aattgatata ccaaaaagtt
      541 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
      601 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
      661 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
      721 cgggaaaatc aaaaaaggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
      781 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
      841 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
      901 atgtagccaa caaatcatac tacaatttgt taactgttag ctataatggc gaaaagccat
      961 gctgaaaggt gtcagctttg caaaaatttg atcttgatca cgtaatcagt acccagaagt
     1021 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
     1081 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt tccatcggtt
     1141 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
     1201 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
     1261 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
     1321 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
     1381 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
     1441 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
     1501 gacacttttg gtaccattac tatcgctgaa cccatcggta ttatttgcgg catcgttccg
     1561 accactaacc cgacttcaac tgctatcttc aaatcactga tcagcctgaa gacccgtaac
     1621 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
     1681 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
     1741 ccttctgttg agctgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
     1801 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggcgta
     1861 ggcgcgggca acactccagt tgttatcgat gaaactgctg atatcaaacg tgcagttgca
     1921 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
     1981 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggtggctat
     2041 ctgttgcagg gtaaagagct gaaagctgtt caggacgtta tcctgaaaaa cggtgcgctg
     2101 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
     2161 ccagaaaaca ccaagattct gatcggtgaa gtaaccgttg ttgatgaaag cgaaccgttc
     2221 gcacatgaaa aactgtcccc gactttggca atgtaccgtg ctaaggattt cgaagacgcg
     2281 gtagaaaaag cagaaaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
     2341 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacggct
     2401 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
     2461 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
     2521 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
     2581 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
     2641 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
     2701 aatggctatg ctgatcagat cacttccgta ttgaaagcag caggcgttga aactgaagtc
     2761 ttcttcgaag tagaagctga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
     2821 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgctgcg
     2881 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagaact ggcgctgcgc
     2941 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggtgtgaa agcgaaaatg
     3001 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
     3061 gacgacacta ctggtcagaa atatccgctg gcagactatg cactgacccc ggatatggcg
     3121 attgtcgacg ccaaccttgt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
     3181 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
     3241 ggtcaggctc tgcaggcact gaaactactg aaagaatatc tgccagcgtc ctaccacgaa
     3301 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcgactat cgcgggtatc
     3361 gcgtttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
     3421 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaac
     3481 gcgaatgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
     3541 cgtcgttatg ctgaaattgc tgaccacctg ggtctgagcg caccgggcga ccgtactgct
     3601 gctaagatcg agaaactgct ggcatggctg gaaacactga aagctgaact gggtattccg
     3661 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
     3721 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
     3781 gagctgaaac agattctgct ggatacttac tacggtcgtg attatgtaga aggcgaaact
     3841 gcagcgaaga aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
     3901 tcagtagcgc tgtctggcaa cataacggcc ccttctgggg taatgccagt cagttaagga
     3961 tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcactcgt ttccggattt
     4021 ttttatgctc attggacagg cccttgattt ggtatcccgt tacgattctc tgcgtaaccc
     4081 actgacttct ctgggggatt acctcgaccc cgaactcatc tctcgttgcc ttgccgaatc
     4141 aggtactgta acgctacgca agcgccgtct tcccctcgaa atgatggtct ggtgtattgt
     4201 tggcatggcg cttgagcgta aagaacctct tcaccagatt gtgaatcgcc tggatatcat
     4261 gctgccgggc aatcgcccct tcgttgcccc cagtgccgtt attcagaccc gccagcgtct
     4321 gggaattgag gctgtccgcc gcgtgttcac gaaaacagcg cagctctggc ataacaccac
     4381 gccgcatccg cactggtgcg gcctgaccct gctggccatc gatggtgtgt tctggcgcac
     4441 accggataca ccagagaacg atgcagcctt cccccgccag acacatgccg ggaacccggc
     4501 gctctacctg caggtcaaaa tggtctgcca gatggaactg accagccatc tgctgacggc
     4561 tgcagccttc ggcacgatga agaacagcga aaatgagctt gctgagcaac ttatagaaca
     4621 aaccggcgat aacaccctga cgttaatgga taaaggttat tactcactgg gactgttaaa
     4681 tacctggagc ctggcgggag aacaccgcca ctggatgatc cctctcagaa agggagcgca
     4741 atatgaagag atcagaaaac tgggtaaagg cgatcatctg gtgaagctga acaccagccc
     4801 gcaggcacga aaaaagtggc cgggactggg aaatgaggtg acagtccgcc tgctgaccgt
     4861 gacgcgcaaa ggaaaagtct gccatctgct gacgtcgatg acggacgcca tgcgcttccc
     4921 cgggacgtat accggagctg atgcgcgatc ttgcaagtat gggacaacta gtgaaattac
     4981 cgacaagaag agaaagggcc ttcccgagag tgataaagga gaggccctgg aaatactcca
     5041 cagccccgaa aaagagccag tcagttgctt aactgactgg cattacccct tctggggccg
     5101 ttttttattt tcccaaagca acttttccat aaaccggcag cattgtcctt catcatattt
     5161 gcgacgatgt ataacgccta cacacagcga tattgtactt tacaggttac aagtcaatgt
     5221 cggtgtataa gcgctctgtg aggcgtataa cggcgttaaa aaactgccga gaagggtgta
     5281 tagcccggaa gaagtgcgta aaacgaactg acagggataa aagcgccctg ctcaccctgt
     5341 cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt taatgagccg
     5401 acttctaacg cctctttata gtgtttacgg catacagaaa cgtagcgttc attaccacca
     5461 ataaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg cagcaccatg
     5521 cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc ggaccatgcc
     5581 agtaagtatt gactgccaat aaataattca cctcgaaaat cggtacgtag accataacaa
     5641 agtacgggta tatcgagttg atcgacaacc tccgataatt catatgcttg ttgtctggtt
     5701 aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg ttccgcacga
     5761 atctcatcaa ataatgatga attttggtta aataattttg caggtgatga caaacctata
     5821 cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata tacgacagtg
     5881 cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga cttacccgca
     5941 ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc aatgatatga
     6001 aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca gtatgcacag
     6061 aagtggtata tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca
     6121 actgcctcgc ttgttataag cgggtaaatg actgccggta actattcaca atctttaacc
     6181 tgttgcgcat gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccctg
     6241 ctcttattgc gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacacg
     6301 actaatacag aagactgaaa ggtcgtcagc ctacgattat ctccccataa aatgtgacat
     6361 gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg gtgcaaatag ggctatatgc
     6421 cgcgtctttt ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca
     6481 attttgaatt ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc
     6541 tcaacaaacc accccaatat aagtttgaga ttactacaat gagcgaagca cttaaaattc
     6601 tgaacaacat ccgtactctt cgtgcgcagg caagagaatg tacacttgaa acgctggaag
     6661 aaatgctgga aaaattagaa gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg
     6721 ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata tcgcgaaatg ctgatcgctg
     6781 acggtattga cccgaacgaa ctgctgaata gccttgctgc cgttaaatct ggcaccaaag
     6841 ctaagcgtgc tcagcgtccg gcaaaatata gctacgttga cgaaaacggc gaaactaaaa
     6901 cctggactgg ccagggccgt actccagctg taatcaaaaa agcaatggat gagcaaggta
     6961 aatccctcga cgatttcctg atcaagcaat aatcttttgt agattgcaca tgctaaaaat
     7021 cccgccatcg gcgggatttt ttattgtccg gtttaagaca atttaataag aataagctat
     7081 aaaaaaaacg gcgtcgattg ctcaacaccg tttcgtggaa aacaccgata cggatgttac
     7141 ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag ggtgttatga
     7201 cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca gtcatggctc
     7261 ttccctttca tatgataggc ttcaaccgtt tctttttcga tcagcatatc aattgcgtcg
     7321 gtgagctgaa tttcatcacc agctccagga ggggtttttg ccagcaacgg ccaaatatcc
     7381 gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc cgcttttggt
     7441 ttttcaacca cgccaaccat cggtacgctt tcacccggcg ctaattcaac gcctttgcaa
     7501 tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg gctatgaccc
     7561 gtttcatcaa agcggcggat catctctgcc aggttatcct gtgataaatc ggattcatat
     7621 tcatccagaa taacgtcagg caaaataaca gccacaggtt catcacccac taccgggtga
     7681 gcacacaata ccgcgtgtcc caggcctttc gccagcccct gacgaacttg cataatagtc
     7741 acgtgcggcg gacaaataga ctgcacttca tcaagcagtt gacgttttac acgtttttcc
     7801 agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt tttagatgag
     7861 tgagtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac gacgtattga
     7921 attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt cgccggcaac
     7981 atcctggttc ctaatcccgc tacggggata acggcttttt tgactttcgt attaatggca
     8041 gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat
     8101 cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt tagcataatt
     8161 aagacaatta cttatacgaa tagcgacgat agtaccacca gccgacatta gcaggtaatg
     8221 caaatttagc ccgtgttatc gtttgctcat tctgcagaca acatcaagcg cagccgacca
     8281 ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg attcaaataa
     8341 gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctgatg ttcgccggta
     8401 tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc gcgatgataa
     8461 tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa atggtttacc
     8521 tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag ctgttcctgt
     8581 aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt atgtccagct
     8641 cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc aatatcaagc
     8701 accaggccgg gtttgtccgc ggcaactaat tgacgataat taacccggca ttgggaaatc
     8761 acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcagc agggttatca
     8821 accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcggga attaaacatg
     8881 ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt aaccggtttc
     8941 agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat attttcagtg
     9001 gcagatatca ccagaactgg tgtctggtca cctctgttgc gtatatgttc cagcagttta
     9061 agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt gaaacctccc
     9121 agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa
     9181 aaccacgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag aatctgtttt
     9241 ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata gtggcatgct
     9301 attgccacct acgcctgtca gaattaactt aagtgtagat aaaaattctg gtcaaatgtt
     9361 ggtgcgtacc aacggcaaaa gttcgtccat tttcttttcc actgccagct gtccggccgc
     9421 aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat
     9481 cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct ccagcacctg
     9541 gatagaagtg gtcataatct ctgttgccgt tggcgctgtc accgcccgac gtgtcgttat
     9601 gctgcctaac ctttctttca gacgcgcatg ccacggcaga gaatcaccat tctcgctatt
     9661 ttcttcactg acattaaagg agagcaaatc ttgttgcatc aaatgagcat cgtgctgcag
     9721 gtcgaccgct atcacaatat cagcccccaa tgcacgcgtg agggaaatag gaattgggtt
     9781 aactacagcg ccatcaacca gccagtagcc gttatgcgca acgggtgcca ttagtcctgg
     9841 aatactgcat gatgcacgaa tagcaagatg gagatcgcct tcagtaaacc ataattcacg
     9901 cccagtactt aaattggtgg caacagccgc gaagcgacgg gaacaatttt cgatctctgt
     9961 ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta acccaccgcg
    10021 ctgccaggag agatccatca ggcgtaaaac atcccaataa ctgaaagagg tcacccaatc
    10081 ttccagcgca gataatcgat cgcatgcata ggcagcgccc accagcgaac caattgaaca
    10141 tcctgcaacg atatcaattt caatacccac tttttttagc gcattaatta cgccaatatg
    10201 cgaccaacct cttgccgcgc cagatcccag cgccagccct atttttatct ttctcattat
    10261 tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg gttaacatag
    10321 tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg tgtctcagct
    10381 ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg tgtctggtga
    10441 aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt ttgtgatgca
    10501 agacgcagat tatttaataa agacctggca tccatcttgt ggagcagcgg cgttacgtgc
    10561 cgaattgatt gccggatttg cgcatactga atggttggga ttgaccgtat ttgaacattg
    10621 ctggcaggac ggcggcgata tcggatttgt cagttttgtt gctcgattta ccgagggtgg
    10681 caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc aatggtacta
    10741 tattgacggt acacgtccgc agtttggccg caacgatccc tgcccttgtg gttcaggtaa
    10801 aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca aacactctca
    10861 acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact atttgtccgg
    10921 accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag ttaaatatcg
    10981 tgcagaacaa tgaatttgta gatcatcgta ccgggcgctt ttttatgcgc acggaactgg
    11041 aagggatttt taatgattcc accctgctgg cagatctcga tagcgcattg ccagaaggct
    11101 ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc actaaagaag
    11161 cacattgcct tggcgatttg ctgatgaaag ccaattacgg cggcctggat gtcgaaatcg
    11221 cggcagttat tggtaaccac gataatttac gttctctggt tgagcgtttt gatattccgt
    11281 ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag atggcggatg
    11341 ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg gtattaacgc
    11401 cggaatttgt ggctcgcttc ccgaataaga tcatcaatat tcaccattcc ttcctgccag
    11461 cgtttatcgg cgcacgtcct tatcaccagg cctatgagcg tggcgtgaag attattggcg
    11521 caaccgctca ctatgtgaat gacaatctgg acgaaggtcc aatcatcatg caggacgtta
    11581 ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga
    11641 aaaacgtctt aagtcgtgcg ctatacaaag tactggcgca gcgcgtcttt gtttacggta
    11701 atcggacgat tattctttaa tcgtcagcca aaataactgg ttagctttaa tccgttacgg
    11761 atgaaaatca cgcaaccagt tcatttttct caacgtaaca ctttacagcg gcgcgtcatt
    11821 tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca ttaccccgtg
    11881 gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag
    11941 gttcgaatcc ttcccccacc accatcactc gattccatta tcaaaccttc ggcttctccc
    12001 ccattcgccg taaaaactgc tgctgtagcc tcaacaaatc cgctaacgtc aacaactgat
    12061 aatccagacc ttgctggctg gcaatttgcg caataatgcg gcttagcgcc gaataatggc
    12121 gatgatgcca gtgaggaaac agatgatggg taaggtgcag attaatccca cccagccagt
    12181 agccaagcca tgcaggttgc ggcgtccaat caaaggtggt agcaaaggta tgcgccagtc
    12241 tgcccacggg cattttgccc tcttctggtg gtaactgcgt atgtcccttc gcccagtgcg
    12301 ttccaataat caacgtcaca aaaatcaatg acgccagcag ctggctgagc agataagtca
    12361 gcaggatggt cataaaacta aaatcagaca aaagccaggg aattatcaga cacagcgcac
    12421 aatgcgccag tttcccggct aagaaatatc cccagccagc aaagcctcgc agggccagat
    12481 gtcgcgtgac cggcgtaaac cctgcgcgat cgagccagtc cactacccag atataccagg
    12541 gaaaggtgag cgcggcgacc agtggccaat agcgatgttg ctggcgcata aaaggtcgcc
    12601 accgttgcca cggcgtctgg cgaagaatgc cattttctgc tgtatctgga tcgtaaaatt
    12661 caatattggt aaaaccgtga tgaaagcgaa cgtggcgcac ccgccagcag tccggatcta
    12721 gccccatagg aaaagcaacc caccggttaa gccgggcatt ggtcttttta ccacgcaaaa
    12781 acgcatcatg cgaagcgtca tgcaccacat tgaccgctaa caacatggcg caaaacatca
    12841 tcgcgagata ccacaacaga tacagtcccc attgacgttg aaataacgcc agtaagtaac
    12901 aggagaaaca agcaataatc aacaccgcca ttttgacgaa tgcccatccg tcggcatacc
    12961 gatgatcgcc tgatttcgcc agataaactt tcgtaccgct ttgcagttgt ttcaccagtt
    13021 gtggatcatc ggcaggaaaa gtgattaacc tgtcgggatt aaacaggttc atactgtttc
    13081 cttagcgatt tacgtcctac gccgattaca aataacaggc aatgggccag cagaataccc
    13141 actaatccct tccagagcag agtactccat gaacacatat agacaataaa atagagcagt
    13201 gacaacgtca cagagcacca caaaatcata ccccaatgct gcccgtcgct catgcccccc
    13261 agcgcaacgg agcctgccgc cagcagaaca aataatacgg cttgctcgct ggtaataaac
    13321 tcaatgccat agccatattg ttgaatgtag ccaagcacca gcataaacag caggatcacc
    13381 cctgccccta ccgcccatgg agaactctgg tagcgacctt caatcgcttt gccaaatgag
    13441 caaaagccca ttaagcgtaa aaatggcaga ttactctcgc gcatcgggtt acaggacaag
    13501 cgactatttt ttacgccgta gcctacgtcg tattccggcc cggcactata cgaaccgaaa
    13561 agtttatccc acaacaaaaa ggtgccaccg taattggtat cggcatattg cttttgattc
    13621 agatgatgga cgcgatgatg cgaaggcgtg atcagcactt tttccagcca gcccagtttc
    13681 ggcgttagtg cgttatggtt aaaaaattgc caactatagt gaataataga aaccgccaaa
    13741 aagacctgta acggcacacc aagcatcgcc agcagcataa aaaacgggat tgaggttaac
    13801 gaggagtacc acgagttccg cacgcctaat gacaggttgt agtgttcccc ctgatgatgc
    13861 accacatgta ccgcccacag cgggcgcatt tcgtgatgcc agcgatgtag ccagtaaaag
    13921 ccgaaatccc acgccaggat agtaaacaac cacagcagga tcaccggcac attatcaaac
    13981 agtccaaggc tgaagtgatt gaccaccagg ccatagcaga gcacttccag gcagcgaaat
    14041 aaccacagca tcatatgacc ggagttgata ttaaaaataa cgtccagcca gtccactttt
    14101 tgccgcccgg tgaattgcaa cacgcccgct tcggctaaca ccaacagcac catcgatatg
    14161 atggggaata ataaatcact catattgtgt cttccttttc tcgcatgttg cgcatatcca
    14221 accgcaaatc gacgccagca acgcgccgcc tactaaatcg ataaataaat gtctgcgtaa
    14281 ttgcagaatc gaaaaggcga ttgctatcgc ccagaccacg ctcattagcg tcagccaacg
    14341 gttccccttt ttagcaatcg cccataccgc caacagcgtt agcgcggcgt gtaaggaggg
    14401 aaaacagttc tggcttgagt caatggcgat cagcctgttg agtgcgcccg cggatatccc
    14461 catctgatca aacgagggat aatgcatggt tgtgggccag agaagataaa atacgccagc
    14521 caccagtgcg gaaatttgca ttgaacgggc cagccagcgc aggtggcaaa gtggcgtgga
    14581 taaataacct aacggcacaa tgaaaaaaaa cgagagatac agccacacag cggaggggga
    14641 aaacgcaatc aaattatcaa ttattgacgg tgttaattga tatccctcac cttgcaggta
    14701 gtcgctaaag ttgtagataa ccccaacagt tccccagccc agcagcatct gtttcaggcg
    14761 tagcagtgaa ttgctcattc ccgcccccac tgacgcacaa tgcgccgccg cttggctgta
    14821 aaagagactg gcacagtttg cagttccagc gaccaaatta attgcccgac agcgatatcc
    14881 tggcgtataa acaaggcatt caacgcctcc cggcaatgct ccagcatcca cagctcgcaa
    14941 tcggcgatta acgtcaattg tgctgacccg gtttgtagca ggcgataatc gactgttagc
    15001 ggcagtgtca ttgccagcac acgactacag gcatcggcaa aaacggtctg aatatcgccg
    15061 gatcttgttg gtagttgcag ttggtcatcc tgccgccctt cgatacgcgc aatcgccatt
    15121 gttgcactgc cacaggggca tggctgttcg cttgccacca gcacatcatc cagtcgataa
    15181 cgcactatcg gctgtgtggt gcgggtaaag tctgtgacga tcggcacgaa acggtgctca
    15241 tccagccatt gcggttcaat gtgaacaaac tcttcgttga gatgtaacgt tccacaccgg
    15301 caggtggaag cgagaaagcc ttctgtcgcc tgataaattt cccccacatt gtggaacacg
    15361 ttgcgcagta attcccgatc ctgcggctcc agaacttccg ccaccgaaat cacttttttg
    15421 acctttagcg tcaattcgcc ggccatcacc tccagcgcca gtgcgcgtaa tacctgagct
    15481 ggcgcgacaa taatcgtggg cgctcgctgt tggagaagtt tgagttgcgc ctgaaatggc
    15541 gctagcagat cgtaaaaatc gagacttaac cagcgattat tcacgctttg atacaggttg
    15601 ttgtccgcgc gcagaaacag cgccacccgc tctttggcaa ataaatcatc gggcaacact
    15661 tttgccagca caccggcggc ccacatctgt tgctcgtgcg ggctaacaac aaaaagtcca
    15721 cgacgcccgg atgtacctga tgaaagccca acgctgaact taccaacgca gggtttgaaa
    15781 tcacgactct gctcactgcg cattgcacaa tcgagaagtt cgtcgcgctt taaacctgcg
    15841 gtattcatct catcgaagtg cgtcatcatt aacgctttat ccatcattgg ccactgatta
    15901 aagggcaacg tcagataacg ctgaaaccac gggctatgag ataagacatt ttgccggaac
    15961 tgatgcagcc tttttgcctg ccagttttcc agtgcctcgc gatcggtgaa atgcagcctg
    16021 cgggtacgga agtagcgcca gatcaacatc aacgggatca taaatccccc tcatggcaca
    16081 aacgaatctc caccacaccg ttttgccaaa gctgatggag ttgcccaagc gtgtggtagt
    16141 aggcgtggct gtcggacata accagatgcg ccagtggtga aggccctcgc agctcccggt
    16201 agttagctgg cgcccatgcc gcatcgccag ccagtaaagt ccagccgttg tcggttaaca
    16261 caaacgcgcc aatatgtccg gcagcatgtc ctggaagcgg cacgagaata atttcgccat
    16321 tgctttctgg cagcaaatag ccgcactcaa acggtgccag ttgcgcgggc aaattcaccg
    16381 atgcgaaact ttcaataaag cgcagtgagg agtcaaaatt ctccgggatt agccctggca
    16441 caaaggcctg gcgcagcgca gtgacaccac gcagagttct tacttgccgc cagccttcac
    16501 cagaacagat gcaggaaaca tcagggaaat cacgcaggcc agcaatatga tcagcgtgaa
    16561 aatgcgacag aataatcgca tcaacatctt tcgctgcgag gccggtggca gaaagttgtc
    16621 gcaccagcgc ctgttgcgga tcgaaataaa ccggcgtgac ctggcgataa agacgaaaca
    16681 caccagattg cgtgtagtgt tcgaaccacg atgaatagcc tgtgtcccac agccaccgtt
    16741 tgtcgcccac ctccagcagc caggcgcggg cggggaattt gcacactttc agccctgccc
    16801 ctttcagcgc aatacagcta atatgggtac accagcccgt ttcaaatgcg gttatttttg
    16861 ccatgttccc tcagccattg tcccgtgatt gcaatacctt gttccatcga aaagcgtggg
    16921 cgatatccca gctctgtaat ggccttttgc gcactcagcg tcatatcgaa acagagtgtt
    16981 ccggcgctgt atcgtgtgat aacaggttcc tgattaacgc acttcccata cagttccagg
    17041 ccacgcgcga tcagagaaag cagcgaccag ggaacagcag caatgcggta actcaagccc
    17101 agttgctggt gcagcaaggc gtcaagcatc accgatagct gctgcggctg gtgattgctg
    17161 atgttgtaaa tactgcccga gcgtaagcct tcaacgttcg ttgccagccg catggcatac
    17221 accacgtttt gcacgaacgt gagatccaat aacgcgtcgc ccccgcgcgg caaacgtaag
    17281 caaccgccgc cctgttgcaa ttgctgcatc agacgaggaa caattacctg gtcatggggg
    17341 ccaaacaaac cacggggacg caaaagaata aatctcgtgg cagaatatcg agaaacagcg
    17401 ccagcaataa cctgctcagc ggcatattta ctgctggcat aatggctgga gaatgctcgc
    17461 gcaagataag tttctggcag gtcatagtga tgttgaaaat caaagtaaac cgcaggcgtg
    17521 gagatatgga taaaccgggg aatattcagc ctgcctgccg cctgtgccag agtgtgggtc
    17581 actttgacat tggcaagcca gaacgccgtt ttgtcgcccc agggcgagga ctttgccgca
    17641 cagtgccaga cggcatcgca gccgtccatc aggcgggcgc agtcatcctc ggttgcctcg
    17701 gttaaatcga gagccacaaa ctgtgcgcct gcgtttgtca ggcttctgcc aacctcacaa
    17761 tttcgccctg ttgccacaac cgactcaccc gattgcaaca aatattgcac ggcatttcgc
    17821 ccaaggccgc tggtcgcccc tgttacgagg atcttcacgg taaaagcacc atgccagcca
    17881 gcgttagtcc cgctgccgtg ccaatcaaca ttacaggttt atccgctgtg aaacgaccgc
    17941 taatcactgc ttcatgcagc gccgtgggga tagaggccgc gacctgattg ccgtaaaaac
    18001 gatacacatc gatgagcgca gattccggca ctcgcagccg tttacgcata tgttcaaggg
    18061 aaagatgact ggcctgatgc ggaacgaccg tgccgatatc agacagttgc aggctgcttt
    18121 tttgcagcaa ccgagaaaaa taatcttcaa taagcgaaga agcaaaacga aacagcggtt
    18181 tcccctgcat atgaaaaaga aagtcgctat cactcacacc agagcgagga ttacgccgcg
    18241 taccgccagc acgaatttca cacagttcgc tgccttcggg atacatttcg atcaggctag
    18301 cgataatccc gctgcgccca tcgccccgct caacaatcgc gcacgccgcg ccatcaccaa
    18361 aaattaatga agactcttca tgatcccagt cgataccgcg tgaagcaata tctgccgaaa
    18421 cgatggcaat gcgtcgatat gctccacagt ttaataatcc ggcagcgact tcaagagcgg
    18481 aaataaagct cacacagcta ctgttgatat caaacccggc aacgcctacg ggcatggctg
    18541 cggctttaag aatatgaatt gcgctacagg gtaatgcctg tacggcaatg gccgatgcac
    18601 aaattagcag atcaatatca gaaggattta aggctgccgc tactaacgca ttatgtaaag
    18661 cctctgccgc cagttcggcc tggctggcgt cgttgcgggc aaaatagcgc cagcaaatac
    18721 cggaacgtcg ctcaacatag ccaactggct tatttaactg aacatccagt tgtttagaag
    18781 tgatttgatc agggggtaag gcgatccccg tggcgattat ttttaacgcc gcggagatcg
    18841 tcggttgatt aggtcgagcc acggtcagtg ctccttcact gctccatagg tggaccaatc
    18901 atatagatga agtacgtttc cctacaatcc gaaaaatcgg cagtgtgtaa ccgccgatgt
    18961 ttggtttaga taatgtttgg tttagataat tagtgacgag cgcgcaccag ctgatactta
    19021 cgcgtcagat actccaccgg gacactccag atgtgtacca gacgcgagaa cgggaacagc
    19081 aggaataacg tcatccccaa caccaggtgc agacggaaga taaacgccac gccatcaaga
    19141 tgttgagaag cgccaccgtg gaaggtcact accgactgcg cccagccaac cagtttcatc
    19201 atctcgctac cgtccatatg ctgagcagag aacggaatgg tcagcaggcc cagcgcgcac
    19261 tggataacga gcagcgacag gatcaggata tccgctccgg tagtcgttgc acgcacgcgt
    19321 gggctgaaca gacggcgttt caacaacagc acgccgccaa tcagacacag tacgccgctg
    19381 gcaccaccag caaacatcgc cattttctgc ttcacttcaa tcggcagcca ggcttcatac
    19441 atccagtgcg gcgtcagcat accgaagaag tgaccgacaa aaatccccag aataccgata
    19501 tggaacaggt tcgacgccag attcatccct ttgcgatcca gcatctggct ggacgccgcg
    19561 cgccaggtat actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg
    19621 gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc
    19681 cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag
    19741 tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaga
    19801 ctgcatccag cgcctgcggc gtatcgtcgc gcgcttcgtc ggcaattttt tccgccactt
    19861 tgtcgctgtc gattgcggta ttcgccagtt tcagcagcag atcaaacagc acggcataac
    19921 ggctttcacg ctgttgcaga cgcgcactca gcaatgccag aatcggcgca atatctttca
    19981 aaccctccac ggcttcgctt tgcggcagct gcgccaggta ctccagatac agcggcagat
    20041 ggtcgggcaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcagat
    20101 ccaccatcgc ctggccgcgg tcacgggatt caccgtgaac atgttcaaat agcaacagtg
    20161 acgtggcgcg gccacggtcg aacagttcgc tgtactgcgc ctgggcatcg agcggatcca
    20221 tcgtcgttaa atcgcgcagg aaaatgccca gcgcatgggc atcctctttt ggcagatttt
    20281 tcgacgcggc aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat
    20341 attcaaggag acgcgataca atcacgagtt cgatcatggg tgcggctccg ttttgctggt
    20401 tacgtcgatg gcatcgatac ggcggctgtt gaacagattg aatttggtat ctgaaccgtg
    20461 gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgcgccag
    20521 ttcacgatga ctgctcggca ccacaaagcg atcttcgtag ttggcaatag ccagataacg
    20581 atacatctcc tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcgac
    20641 tttaccgtca acggtttcag cacgtttgta atgacgcatc gccagcatac gtttcagcgc
    20701 gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat
    20761 acgcaggctt tcgacgtctg gcagaatgcc gttgctgccc agttcacccg cgtctgccgc
    20821 agactgaatc ggcgacagag gcggcacgta ccagaccatc ggcaatgtgc gatattccgg
    20881 atgcagcggc agcgccagtt tccattccat cgccatttta taaactggcg actgctgcgc
    20941 agcctcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc
    21001 gttcggatcg aggaacacat ccagctgacg ctggtagaga tctttctcgt tctcggtact
    21061 tgccgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc
    21121 gacacaggtt tctgaacata ttgtcggctg gccggcttca atacgcggat agcagaagat
    21181 gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt
    21241 gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc cgtcttcttc
    21301 acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca gacagtgttc
    21361 gcacaggcgc ggcaaataca tcatgaaggt gttttcgaac tggctataca tcgccttctg
    21421 gatgttgtcg aagttcttgt ctttcgccag cttgtcaaac tcaccgccca ggtcatcttc
    21481 ccagttcggc cctttttcga ttttcgccat ccgttcgccg gtaatcagcg aacgcggacg
    21541 ggcaatcggc tgtgatttgc tgccttccgg cgcggtatgc aggttctgat aatcaaaatc
    21601 aaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag
    21661 cagcatggca cggttaccca tgcgcggctg cagtttgccg ttgattttac ggatccagcc
    21721 gcctttgtat ttttcctggt tttcccagtc agtcgggaag ccctggcccg gcttggtttc
    21781 cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt
    21841 cactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga
    21901 acgaattttc attttacgct ctcctgtacc tggtcattgc cttcgccatc taaccagtca
    21961 atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag
    22021 tagttaaagc catatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg
    22081 atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg
    22141 ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg
    22201 acaaccgcac gggcagtcag tgcaccgttg ctgttgaaga cttcaatcca gtcgttatcg
    22261 gcgataccca gctctttggc atcggcttca ctcagccaga ccaccggacc accgcgacct
    22321 aaagtcagca tcaacaggtt gtcgctgtag gtggagtgga taccccactt ctggtgtggc
    22381 gtcaggaagt tgagcgcttt ttccgggttg ccattggatt tctggcccat cacttctttc
    22441 accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc
    22501 cattggtgat cctgatacag ctgctgacgg ccagagagcg tacgccacgg gatcagctcg
    22561 tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttcaaggcc agaccatgtc
    22621 gggctggaga tgattttgcg cggctgtgcc tgaatatcgc ggaagcgaat cttctcgtct
    22681 tctttattca gcgccagatg cgtatggtca cggccggtaa actcgctcag ggcagcccag
    22741 gcttttacag ctacctgacc gttggtttcc ggtgccagcg tcaggatcat ctctgccgca
    22801 tcaattgcgg tattgagcat cggctgacct tttgccggac cttccgcttt tgtgtagttg
    22861 agcttacgca gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccgtta
    22921 ccgattttct ccatcagcgg gccgatagag gtaaagcgtt cgtaagttgc cggataatcg
    22981 cgctctacga ccataatgtg cggtgcggtt ttacctggga tcaggtcgca ttcgcctttt
    23041 ttccagtctt tcacatccag cggttgcgcc agttcagcgg cagagtcgtg ctggataggc
    23101 agcgtgacga tgtcagtttc tttacccaga tgaccaacgc acacttcgga gaatttcttc
    23161 gcgatggctt tgtagatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca
    23221 gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag
    23281 gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc
    23341 gtcaccacca gatccagctt gccttccaga ccgttgtcct gccagtccac ttcttccggc
    23401 ttcacgccgc cctgctgccc caggtctttg ccctggatac cgtgctccgt ccccagcagg
    23461 tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagatg
    23521 aacaggttac gcgggtggtt tttaccgttt tccggctgtt ccgcagcgaa acggatagaa
    23581 ccctctttca gggatttcac cgtatagtcc accggattca tcccggcttt ttctgcttcg
    23641 cgagcgatag tcagcgggtt agtgcctaac tgcggcgcag acggcagcca gcccatgcgt
    23701 tctgcacgga cgttaaagtc gatcaagtgt ccggtatagc gagatttatc cgccatcggc
    23761 gacagcagtt cttccgcagt cacggtttca taacgccact ggctggagtg gttatagaaa
    23821 taagaggtac tgttcatgtg acgcgccgga cgctgccagt cgagggcaaa cgccagcggc
    23881 tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca gccgcccccg
    23941 ctctgaccga cacaaccgca gaaaatcagc atgttgatca gaccacgata gttcatgtcc
    24001 aggtgatacc agtggttcag ccctgcgccg acgataatca tcgaacgacc gtgcgtttta
    24061 tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta
    24121 atctgctctg cccaggcagg ggtgtaagct ttcacatcgt catagctggt tgcacagtta
    24181 acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc
    24241 accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagctt atgcagcagc
    24301 acgttttcca gttccacttt gttgaagtgt tccgtgccat cgccaccaaa gtacgggaag
    24361 cccacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg
    24421 ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca
    24481 atagaaccgt tcggcgcaac catttcacca ttggtattaa aggcgacagt tttccattcc
    24541 ggattgtttt cctggcccag tgcatccacc agatcagcag cgcgcagcat acgacccgca
    24601 gcgtagtaac cgtcgcgctc ttccagcatc accagcatcg gcatgtcggt gtagcggcgc
    24661 acatagtcgg tgaaatactg gcttgggttg tcgaggtgga attcacgcag cattacgtga
    24721 cccattgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac
    24781 agtttggcga tttcagcgta gtctggtgtg acggcaacag ttttggtccc tttgtaacgc
    24841 acttcagtaa agaagtgagc atccggggta cgcgtctgcg gcacgtttga accccaggcg
    24901 atgatgtagc tggagttgta ccagtcagca gattccggta cgtcagtttg ctcaccccag
    24961 gtttgcggag acgcaggagg caggtcgcag taccagtcgt agaagcttaa gcaagtaccg
    25021 ccaatcagcg agagatagcg cgcacccgat gcgtaagaaa ccatcgacat tgccggaatt
    25081 ggcgagaagc cagcgacacg gtccgggccg taatttttga tggtgtaaac gttagatgcg
    25141 gcgatcagtt cgttcacttc ctgccaggaa gaacgaacaa aaccaccgcg tccacgcgcc
    25201 tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga
    25261 tcgctatgca gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg
    25321 tatttcaggc ggttagcgct gtaaagatac caggagtagc tggcaccgcg cgggcagccg
    25381 cgaggttcat ggtttggcag gtccgggcga gtacgcggat agtcagtctg ctgggtttcc
    25441 caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc
    25501 ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag
    25561 tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg
    25621 aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc
    25681 cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg
    25741 gaattgataa cgatcaagag tgatggggat gaaaaataaa gtaaattcct ggaatacccc
//
LOCUS       DAPQZD010000057        25746 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPQZD010000057 DAPQZD010000000
VERSION     DAPQZD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.057"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            241..1539
                     /gene="kgtP"
                     /locus_tag="RO454_002315"
     CDS             241..1539
                     /gene="kgtP"
                     /locus_tag="RO454_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDY0934142.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1536..1859)
                     /locus_tag="RO454_002316"
     CDS             complement(1536..1859)
                     /locus_tag="RO454_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDY0934143.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="RO454_002317"
     CDS             complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="RO454_002317"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDY0934144.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3374..6034)
                     /gene="pat"
                     /locus_tag="RO454_002318"
     CDS             complement(3374..6034)
                     /gene="pat"
                     /locus_tag="RO454_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDY0934145.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6066..6764)
                     /gene="tapT"
                     /locus_tag="RO454_002319"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6066..6764)
                     /gene="tapT"
                     /locus_tag="RO454_002319"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDY0934146.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="RO454_002320"
     CDS             complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="RO454_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDY0934147.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7459..8496
                     /locus_tag="RO454_002321"
     CDS             7459..8496
                     /locus_tag="RO454_002321"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDY0934148.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8544..9233)
                     /gene="ung"
                     /locus_tag="RO454_002322"
     CDS             complement(8544..9233)
                     /gene="ung"
                     /locus_tag="RO454_002322"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDY0934149.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9538..9921
                     /gene="grcA"
                     /locus_tag="RO454_002323"
     CDS             9538..9921
                     /gene="grcA"
                     /locus_tag="RO454_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDY0934150.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9977..10564)
                     /gene="eamB"
                     /locus_tag="RO454_002324"
     CDS             complement(9977..10564)
                     /gene="eamB"
                     /locus_tag="RO454_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDY0934151.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10667..11548
                     /locus_tag="RO454_002325"
     CDS             10667..11548
                     /locus_tag="RO454_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0934152.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11581..12915)
                     /gene="srmB"
                     /locus_tag="RO454_002326"
     CDS             complement(11581..12915)
                     /gene="srmB"
                     /locus_tag="RO454_002326"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDY0934153.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13047..13784
                     /gene="trmN"
                     /locus_tag="RO454_002327"
     CDS             13047..13784
                     /gene="trmN"
                     /locus_tag="RO454_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDY0934154.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13769..15391)
                     /gene="nadB"
                     /locus_tag="RO454_002328"
     CDS             complement(13769..15391)
                     /gene="nadB"
                     /locus_tag="RO454_002328"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDY0934155.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            15647..15802
                     /gene="rseD"
                     /locus_tag="RO454_002329"
     CDS             15647..15802
                     /gene="rseD"
                     /locus_tag="RO454_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDY0934156.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15799..16374
                     /gene="rpoE"
                     /locus_tag="RO454_002330"
     CDS             15799..16374
                     /gene="rpoE"
                     /locus_tag="RO454_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDY0934157.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16407..17057
                     /gene="rseA"
                     /locus_tag="RO454_002331"
     CDS             16407..17057
                     /gene="rseA"
                     /locus_tag="RO454_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDY0934158.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17057..18013
                     /gene="rseB"
                     /locus_tag="RO454_002332"
     CDS             17057..18013
                     /gene="rseB"
                     /locus_tag="RO454_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDY0934159.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            18010..18489
                     /gene="rseC"
                     /locus_tag="RO454_002333"
     CDS             18010..18489
                     /gene="rseC"
                     /locus_tag="RO454_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDY0934160.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18687..20486
                     /gene="lepA"
                     /locus_tag="RO454_002334"
     CDS             18687..20486
                     /gene="lepA"
                     /locus_tag="RO454_002334"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDY0934161.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTEQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20502..21476
                     /gene="lepB"
                     /locus_tag="RO454_002335"
     CDS             20502..21476
                     /gene="lepB"
                     /locus_tag="RO454_002335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDY0934162.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21748..22428
                     /gene="rnc"
                     /locus_tag="RO454_002336"
     CDS             21748..22428
                     /gene="rnc"
                     /locus_tag="RO454_002336"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDY0934163.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22425..23330
                     /gene="era"
                     /locus_tag="RO454_002337"
     CDS             22425..23330
                     /gene="era"
                     /locus_tag="RO454_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDY0934164.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23342..24070
                     /gene="recO"
                     /locus_tag="RO454_002338"
     CDS             23342..24070
                     /gene="recO"
                     /locus_tag="RO454_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDY0934165.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24082..24813
                     /gene="pdxJ"
                     /locus_tag="RO454_002339"
     CDS             24082..24813
                     /gene="pdxJ"
                     /locus_tag="RO454_002339"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDY0934166.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24813..25193
                     /locus_tag="RO454_002340"
     CDS             24813..25193
                     /locus_tag="RO454_002340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDY0934167.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25614..25694
                     /gene="shoB"
                     /locus_tag="RO454_002341"
     CDS             25614..25694
                     /gene="shoB"
                     /locus_tag="RO454_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDY0934168.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
BASE COUNT         6335 a         6394 c         6772 g         6245 t
ORIGIN      
        1 ttatactccc acataagcca gattcaacag cgaatacgtc ttccccaaat tgcccacttc
       61 catacttcct ccttaccaga aatctatcct taagctcctt aataaccatt ttcctgctaa
      121 ctaaattcat ggttaaggtt gtataatgat gcgcaacaaa tgtataacat tttctttacc
      181 aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag acataatggc
      241 atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac tcgtcgccgc
      301 atttgggcaa ttgttggggc ctcttcaggt aatctggtcg agtggttcga tttctatgtc
      361 tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa cacgacgact
      421 caactactgc aaacagcagg tgtttttgct gcaggatttc tgatgcgccc aataggcggt
      481 tggctgtttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg
      541 tgtatgatgt gtttcggctc actggttatc gcctgtttgc caggctacga aacaatcggt
      601 acctgggctc cggcattatt gcttctcgcg cgtttattcc agggattatc cgttggcgga
      661 gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg caaaggtttt
      721 tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct actggttgtc
      781 gtggttttac aacacaccat ggaagacgct gtactcagag agtggggatg gcgtattcct
      841 ttcgcgttag gagctgtgtt agctgttgtg gcgttgcggt tacgtcgtca gttagatgaa
      901 acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg attatggcgc
      961 aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct ttgtttctat
     1021 accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca tgccaacgtg
     1081 gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc aattattggc
     1141 gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc tctggcaacc
     1201 atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta tgccgctttt
     1261 ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag tggaatactg
     1321 aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc atatgcgttc
     1381 gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa atcaatagga
     1441 atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt tctggtttca
     1501 ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag ttgccagacg
     1561 gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct ccagccgctc
     1621 ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga gaacatcaat
     1681 ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc atttccggcg
     1741 gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct ccagttatcg
     1801 ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag aatacgcaaa
     1861 acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag gatgcggcta
     1921 attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac cttcaccgga
     1981 taatcggcaa tactttgcag atcgcggtag tgcttaacga tggtggtatg ctcgcggatc
     2041 agatccagtt ctttctctcg ctgtggcgca agctccagtt gcggatcgtg gatcaaaatg
     2101 gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt gatcaaaatc
     2161 cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc tttccataac
     2221 cgaactacta actggtcagt gttgacgtaa tactgtaaac gactcaggaa acgacgcaga
     2281 ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc ttccggaatg
     2341 tagaagtcat tcgcggtttt atcaccaaca ataatttcga cctttttccc ttcgcgcagc
     2401 aactggataa tattgcgcac aaggattgct ggtaggttga agtaaggtgt gcagatggtt
     2461 agtttctgct cggcacaagg cataagatgg aaaatagtct tgttcaacag actcgatttc
     2521 cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga
     2581 taagcggcat cacgcagctc ctggcggaac aggcgaatgt cgttcttgat ttccgggctt
     2641 tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat attctgtgta
     2701 acccattcaa acataatgtc tgacatctta cggttacgga tcagatgata acggtcgtag
     2761 cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct ataaagtacg
     2821 ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg ggtattgatt
     2881 ggaacgccat aaaccggaac atttacgccc ggattttcct gcgccatgcg gcagtaccag
     2941 tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg
     3001 accagcaccc gcacatccag ttccggacgc tgccttttag cctcatacaa cgcgttcaga
     3061 atgcctttgc caccgtcatc ctgttcgaga tacagggcaa caatgcaaat gcgctgcttc
     3121 gcgctggcta ttttttccag caacgtctcc cggaagtcgg cgggagcgta aaagaaatcg
     3181 acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg atgtttatta
     3241 cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag ggtcctctgt
     3301 gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt ggtcaacatt
     3361 tccagtacct tactcatgat tcctcgcgct gggcaagact tagcgtaagc ccaacgatcc
     3421 cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggccagcgcc accatgccac
     3481 gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc gtataggtaa
     3541 tcaacttttc cattaagcgt cgacctaagc ctaacccttt aagatccgag cgaaccagta
     3601 cggcaaattc ggcatcgatg ttatcagggt cggagatcgc acgtgtgacg ccgaggatct
     3661 cttccgtttg atcaattcgt cgtaccgcta caaatgccat ttcccgatcg tagtcgatct
     3721 gcgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag tagcggtaat
     3781 aaagatcttc tttggtgact cgcgaaatga attgctgaag ctgtggctca tcttctggca
     3841 aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct tccagctgat
     3901 gcggataagg gcgcacagcc agtcggctct cgttatcacc ttcaaacggc gcgatatcca
     3961 gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga atatccagac
     4021 gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc tggctcaagc
     4081 ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt ttgatcccct
     4141 gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg acggcttgat
     4201 cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg aaaaccggat
     4261 cgtgctcaac cacaacccgc aactcctgag cgccagcacg gttagccata ctttgcacca
     4321 acaggccgtg gacccgcgcc tgcggccagg ccatttttac gcgatcgaaa atagcgtttg
     4381 ccgcttgctg gacttcattg gctgtacgta ggtagagcat gacgccctga acttccgatt
     4441 tatgtggaat atccggcgag cgcaatttca gtgccaccgg atagccaatc tgttcggcaa
     4501 tatgtaccgc ttcggtgcta tcactggcaa tccaggtagg gagagtattc ataccatacg
     4561 cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg gcaatcgcct
     4621 gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc aacgccggcg
     4681 tttcgcgtag ttgcttctga ttacgccggt actcgaccat atgcataaaa gcagtgatgg
     4741 ttccttccgg agtacggtag gtcggcagcc cggcttcgct gaataaacgt cgtgcctctt
     4801 gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg cggggatgat
     4861 gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg
     4921 aatgaataac catcagcgca tcaaaatcct ggctgtggag cagaatgtcc agcgttttga
     4981 cataatgctc gctgctggcg tcatcgcgca gatcgagcgg gttagagatt gccacatgcc
     5041 ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc agcttgccat
     5101 tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg ctgataatca
     5161 tcaaccggtc gccacgcagc gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt
     5221 cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agctgcatcc cacgccggat
     5281 ccattcctgc cgtggtgttc aacagacgtt gagccgccgg gctacgtccg cttttaatca
     5341 ccaaaatcgg tttattgcgc gaggcactac gggctgccga aacaaagcgt cgcgcgtcgc
     5401 ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgcgcc agatagtcga
     5461 gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag gaaaagccca
     5521 tctcacgctg ttgcgcccag tcgaggatgg tgttggagac agcagccgat tgcgaaataa
     5581 acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga ccttgccagg
     5641 gagccagtaa gcccagactg tttggtccta gcaggcgcat gttatggcgc agggcgcagg
     5701 cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt ttacagcctt
     5761 tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat aaaaccgcaa
     5821 ggtcgggtgt aaagggcaag ttggcaatat ccggccaggc caacacaccc aacaccgctt
     5881 tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc aggttacgca
     5941 tcatcaggta acctgcgcga ttgggtttca tcgatgcgcc aattaccgca atcgattttg
     6001 gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga agtgaccgaa
     6061 tgattttaaa cgctttctaa ctgttctgct gtgatgcttc ccggatgttg cgtttttcct
     6121 gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgtcgc cgccctggta
     6181 tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg gccttcagcc
     6241 tgggcttcac gcaggcgata ggcagaaagc cgagaaagat cgacggaaat gacgggaaga
     6301 ttatccagat acggactttt acgaaacatc ttgcgagctt ctggccaggt accatcgagc
     6361 atgataaata gcggaggctt atcggcaggt ggtgtgaaga tcacttcccg ttgctcatca
     6421 gcatacgaag cgggaaagac caccattggc tgatagtctg ggttttgcac cagatccagc
     6481 aaatcttgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg caaaatatca
     6541 gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat cagcaaacag
     6601 aagtgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt ctctggcaaa
     6661 agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc
     6721 gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg tcataagaga gcgtcgaaaa
     6781 aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa gattaaagag
     6841 attcgttcag ccagctgtcg aacggcgctt tcggtactgc gccattaagc atgtcgacaa
     6901 cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag cgactgctca
     6961 attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta cgctcttgcg
     7021 cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt gcccagaagt
     7081 cgatcaccac aggtagatca tctttcagca atttgtcgag cgtttcaccg gtcgcattaa
     7141 tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc
     7201 gatcgtcggg aatgcgattg atggcctgac aatgggtaca aacggtattc ataactaacc
     7261 tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat atgttacata
     7321 ttatcgggga cattgttcca gacaacaatc tgttttattc aatgggtatg atagacacag
     7381 gctttggaac gaaatcatgg cgaaccgcgc gcacttcggg taatctgcgc gcttcgcgca
     7441 gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg
     7501 atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg taccgggaaa
     7561 gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac
     7621 aaacagagtc agcaccgtga ccgcaagtgg gaagattcgc cgtggcgcac ggtttcccgc
     7681 gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg taaaagtttt
     7741 attgatccgg aagttttgcg tcgtcagcgt gcggaagaaa cccgcgtcta cggcgaaaac
     7801 gcctgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg gtttatccag
     7861 agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg caaagcgtac
     7921 catgtggtgg atgaagcgga actgacaaaa gcgtcaggca cggaacatca cggcggcgtt
     7981 tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag ccaggcaggc
     8041 gcgcaggatt gtgtactggc cctggaaaac gaatctaacc cgcataacct gggtggcatg
     8101 atgcgtagct gtgcgcactt cggcgtgaaa ggtgttgtgg tgcaggatgc agcactgctg
     8161 gagtctgggg cggctatccg taccgcagaa ggcggcgcag agcacgttca gccgattact
     8221 ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg cgggttacac cgtagtgaca
     8281 acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa aatggtactg
     8341 gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa cgatctgcgc
     8401 gtgaagattg atggtactgg caacgttgcc gggctgaata tctctgtcgc aaccggcgtt
     8461 cttcttggcg aatggtggcg tcagaataaa gcctgataaa tcagccgggt ggcaactctg
     8521 ccatccggca ttttcccgca aatttactca ctctctgccg gtaatactgg catccagtca
     8581 atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg gttgcagcca
     8641 aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac atgatggcgt
     8701 tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa aaacaccacg
     8761 ccttcgcgat gctgatttat gaggctgatg actttatcag taaacgtctc ccagccgagg
     8821 ctggcgtggg aatgcgcctg accagcgcga accgtcaaga cggtattgag cagcagaaca
     8881 ccctgacgcg cccagctttc aagataacca tgattagggc gggtgaaacc gggaatagta
     8941 ttttccagct ctttatacat attcagcagt gacggtggtg tcgcaatgcc ggggcgaacg
     9001 gaaaatgcca gaccatgcgc ctgtcctggt ccgtgataag gatcctgacc gagaatcacc
     9061 actttaacgt cacccaactc cgtaaagcgg aacgcgttaa agacatcttt ttgtggtgga
     9121 tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt aagaaaatag
     9181 ggttgctgct tctcttcagc cagcacatca tgccaggtta attcgttagc catctcgctc
     9241 tcctgcgaat ctttaatccg cctagcttaa ctgcttcttt ctacagaaca aaatcatgct
     9301 cattttatag acaaaaagac atgcaaaaaa atgtaaattg gtaaaatttc acatctgcat
     9361 aagttgatgt aaaacaataa aatccatttg tgaccacact tgatatgtgg tttttattga
     9421 tttaaatcaa agaatcaagg gtgtttgagg agtatatata cactcaagca acaatggttt
     9481 taccaattgg ccgcgacagg ctgaacaaat caaataattt tgccggggag gcatcacatg
     9541 attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc tttctggttg
     9601 ctggacagcg aaaaaggcga agcgcgttgc atcgttgcga aagcaggttt tgcagaagac
     9661 gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc agtagaagtg
     9721 aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct gcgtcgtgaa
     9781 actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat ccgtgtatcc
     9841 ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt tatcgctcgt
     9901 acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag agggcgacct
     9961 cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc aacagggcaa
    10021 gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga tgccccgcca
    10081 gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg acgccaacta
    10141 cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta acaccgtaca
    10201 aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg attggttttg
    10261 cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc cagacaatat
    10321 atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact gccagtgaaa
    10381 atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc cctgccagca
    10441 cacgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga atattgttcg
    10501 gtcctggcgt catggcggta atcagggtgt aagtccaaaa agcacttaaa agggtcggtg
    10561 tcacttgtta tctcctctgt tttggtagac agtacccgac acggaacatt taaaaaaatg
    10621 aatgttttta cattctaaat tcgatatttt cgataggttt ggggttatgg atctgcgccg
    10681 ttttattacg cttaaaaccg tggtggaaga gggttccttt ttgcgagctt cgcaaaaatt
    10741 gtgctgtaca caatcgacgg tgacttttca tattcagcag cttgagcagg agttctcggt
    10801 acaattgttt gagaaaattg gtcgacgaat gtgccttacc cgcgaaggaa aaaagctgct
    10861 gccgcacatc tatgaactta cccgagtgat ggatacgctt cgtgaggctg ctaaaaaaga
    10921 gtcggatccg gacggggaac tacgcgttat ttcaggcgaa acgttgctct cttatcgaat
    10981 gccgcaggtg ttgcagcgtt ttcggcaacg cgctccaaaa gtgcgtttat cgttgcagtc
    11041 gctgaactgt tacgtgatcc gtgatgcgct actgaatgat gaggccgatg tcggggtttt
    11101 ttatcgcgta gggaatgatg atgctctgaa ccgacgagag ttgggtgaac aaccgctggt
    11161 gctggtggct tcaccgcaaa ttgcagatgt cgattttact gagccgggaa gacataacgc
    11221 ctgtagcttt attatcaacg aaccacaatg tgtcttccgg cagatatttg agagcacgct
    11281 gcgccagcgg cggatcacgg tggaaaacac cattgagctt ataagtatcg aaagcatcaa
    11341 gcgttgtgtc gcggcaaata tcggagtcag ttacctgccg cgttttgcgg tagtaaaaga
    11401 gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa cagtcgcaga ccattacggc
    11461 aatgtgcgct caccatgccg gaaaagcggt tagcccggcg atgcacactt ttattcagtg
    11521 tgttgaagag tgttttttgc cgggataaaa acaatgccgg gcttaaaccc ggcattgaga
    11581 ttactcttct gtcgtttgcg gtggcacgcc cgttccgctt ggtttacggc gcttaccaat
    11641 atttttagtg tcgcgatggc gttttttcac ccgcggcttc tctttctctt tagctttttt
    11701 cttctcggca cgtttagcca acactttctt cgatggcttg ccggtctgct tctcgcttgg
    11761 cgcacgcgtt ttcggacgta actcatcaat tacgcgagct ttaattggct cttcaatata
    11821 acggccaact ttgcccagca gcagatggtc atgggcttcc accagcgaaa tcgcggtgcc
    11881 tttacgaccg gcgcgcgcgg tacgtccgat acggtgcaaa taagtatcgc cactgcgcgg
    11941 catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg ataccgcgcg cggcaacatc
    12001 ggtagcaacc agtacgttta cgcggccttc ggtcaagcgt ttaatcgctt cgttacgctt
    12061 gccttgtacc atctcacctt cgagatagca gttgttgatg cctgcttcgc gcagccagtt
    12121 tgccagctca tgcacacgct cacgcttacg cacaaacaca attgagcggg tcgcttccgg
    12181 ctgttttaac agatgcacca gcaacgcggt tttatgctca agatcatcgg cgcggtaata
    12241 ccactgatga atttttttgc gctcacgggt ggacggattg gcagaaactt ccaccggatc
    12301 ttccaacaga cgctcggcaa agtcctgaat cgcatcgcct tccagcgttg ccgaaaagag
    12361 cagggtctgt ttacgccagc gcgtttcgcc agcaatatgt tcgatatcct gggcgaagcc
    12421 catatccagc atacggtctg cttcgtcgag gatcagcgtt tcaaccgcgc ggcaatcgaa
    12481 gttctcttct tttatgtatt gcagcagacg tccggtcgtg gcgaccacaa tgtcctggtt
    12541 ttcgctgaac acttctgcgt ggttcatata ggccacgccg ccggtgatgg tggcaatatc
    12601 cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc acctgcatcg ccagctcgcg
    12661 agttggggtg aggatcaaaa tacgcggcgg accggatttc ttacgtggga aatcgagcag
    12721 gtgctgcaac gctggcagca gatacgccgc cgttttaccg gtgcctgtcg gcgcagaacc
    12781 gagtacatca cggccatcga gcgcaggcgg aatggcggca gcctgaatgg cggtcgggcg
    12841 agtgaaacct ttatcctgga gggcttccag caggctttcg tcgagttcaa gttcggaaaa
    12901 agtcgttaca gtcatgttct acctctgtgt ggggcgctga ttatagacgt tacggctgca
    12961 atcttcatct gtttgtatgg atatcgcttc tcgggtattg ttctcacccg gtgctatcgc
    13021 cgtttttcct gcaaggtttt attttcatgt cacagtctac atccgtgttt cgtcgtaatg
    13081 gatttacttt taaacagttt tttgttgctc acgatcgctg tgcgatgaaa gtgggaacgg
    13141 atggcatttt gctgggcgca tgggcaccgg tagctggggt aaaacgttgc cttgatatcg
    13201 gcgcgggtag cgggttgctg gcattaatgc tggcgcagcg aacagatgac agcgtgatga
    13261 ttgatgcagt tgaactggaa agtgaagctg ccgcgcaagc gcaggaaaat atcaaccagt
    13321 ccccgtgggc agagcggatt aatgtccata cggcggatat tctgcagtgg atcacacagc
    13381 agacagtacg cttcgattta atcatcagca acccacctta ctatcagcag ggagtagagt
    13441 gcgcgacacc tcaacgggaa caggctcgct ataccactac gcttgatcac ccatcgttgc
    13501 tgacttgtgc ggcggagtgc attaccgaag agggattttt ctgcgtggtg ctgccagagc
    13561 agattggtaa tggttttact gaactggcat taagtatggg ctggcattta cgtttgcgga
    13621 cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt gctgctggca ttctccccgc
    13681 aggcgggaga gtgctttagc gatcgcttag tcattcgtgg gccagaccag aactattccg
    13741 aagcgtatac ggcgctgacc caggcttttt atctgtttat gtaatgattg ccggcagaaa
    13801 ggatcgatgg accggaatgc gtgagcagtt ccggataatc cagcgtgaaa tgcaggccac
    13861 ggctctcctt gcgcatcatc gcacagcgaa caatcaactc ggcaacctgt accagattac
    13921 gcagctccag caaattattt gagacgcgga aatgggcgta atattcgtct atttcttgtt
    13981 ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt cgttgtgcga acaatgccaa
    14041 cgtaatccca cataaacaga cgtagctcgt gccagttatg ctgaattact acccgttcgt
    14101 cagggttctc aacgcggctt tcatcccacg gcggtaacgt actgacgccg tgggcataag
    14161 gtatacgtct gctgatatct tctgccgccg accagccata gaccagacac tccagcaatg
    14221 aattcgaggc catgcggtta gcgccgtgta agccggtata actcacttcg ccaatggcat
    14281 acaaaccctc gacgtccgta cgcccatgat catcaaccat tacaccaccg caggtataat
    14341 gtgcagcagg cacaatcggc acgggttctt tcgtgagatc tatccccagc ccgagcaatt
    14401 tttcataaat catcgggaaa tgctggcgaa taaaatcggc gggcttatgg ctgatgtcga
    14461 ggaacataca atctgcgccg aggcgtttca tttcatggtc aatggcgcgg gcgacaatat
    14521 cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat aaaacgcgtg ccatccgggc
    14581 gcttgagata agcgccttcg ccgcgcagtg cttccgttaa caggaaattg cgtgcctgtg
    14641 ggtgatacag cgcggtaggg tggaactgat taaattcgag attggcaacc cggcagcctg
    14701 cgcgccacgc catagcaatg ccatcgccag aagaaatatc cggattggtg gtgtactgat
    14761 aaacttttga agcaccgccg gttgccagta ccactgcttt tgcgtggcag gtttcaaccg
    14821 tttctttatt tcggttccat acccacgcgc caacaacccg tcgcgtgccc ggcaggccaa
    14881 ttttgtcaga aacaatcaga tcaaccgcgt tgctgcgctc cagcacgcga atattcggat
    14941 ggttctgcgc cttgcccacc agcgtggttt ctacttctct accggtggcg tcggcggcat
    15001 gaagaatacg acggtgacta tgtccacctt cacgagtcag atggtagctt tcttcgccat
    15061 tcggctgaat gtgggtatca aacaacaccc cctggtcgat tagccattgc acacaggatc
    15121 gtgcattact ggcgacaaat tcaactgcat ggcgatcgca aataccagcc ccggcaatca
    15181 atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc aaacacggcg gcaataccgc
    15241 cctgggcata aaatgttgaa ccttccgtta ccgggccttt acttagaacg atgacctgat
    15301 gctggtcagc caggcgtagc gccagtgaaa gtccagctgc gccgctaccg ataatcaaca
    15361 cgtcacatga atgttcaggg agagtattca ttttctttgt ttaatttact aaacactgtt
    15421 tggtcagcat aacatcatgt tgtgcaaata aacacctgct attttaatat ttgttacagt
    15481 gactaaacac gctggctcag ggcggcgaga aaaagagaag ttactggctg gtggaggatt
    15541 aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc agttaaatgg gcatttctac
    15601 acagataatg cgatgttcag attctgtaga cttataatga tagataatga tccgtctaca
    15661 gcatgacaaa caaaaacaga tgcgttacgg aactttacaa aaacgagaca ctctaaccct
    15721 ttgcttgctc aaattgcagc taatggagtg gcgtttcgat agcgcgtgga aatttggttt
    15781 ggggagactt tacctcggat gagcgagcag ttaacggacc aggtcctggt tgaacgggtc
    15841 cagaagggag atcagaaagc ctttaactta ctggtagtgc gctatcagca taaagtggcg
    15901 agtctggttt cccgctatgt gccgtcgggt gatgttcccg atgtggtaca agaagctttt
    15961 attaaagcct atcgtgcgct ggattcgttc cggggagata gcgcttttta tacatggctg
    16021 tatcggattg ctgtaaatac agcgaaaaat tacctggttg ctcaggggcg tcgtccacct
    16081 tccagtgatg tggatgccat tgaagctgaa aacttcgaaa gtggcggcgc gttgaaagaa
    16141 atttcgaacc ctgagaactt aatgttgtca gaagaactga gacagatagt tttccgaact
    16201 attgagtccc tcccggaaga tttacgcatg gcaataacct tgcgggagct ggatggcctg
    16261 agctatgaag agatagccgc tatcatggat tgtccggtgg gtacggtgcg ttcacgtatc
    16321 ttccgagcga gggaagctat tgataacaaa gttcaaccgc ttatcaggcg ttgacgatag
    16381 cgggatactg gataagggta ttaggcatgc agaaagaaca actttccgct ttaatggatg
    16441 gcgaaacgct ggatagtgag ctgcttaacg aactggctca taacccagaa atgcagaaaa
    16501 cctgggaaag ctatcactta atccgtgact caatgcgggg tgatactccc gaggtgctcc
    16561 atttcgatat ctcttcacgc gtgatggccg ccattgaaga agagccagta cgtcaaccgg
    16621 cgacattgat cccggaagcc cagcctgcgc cgcatcaatg gcagaaaatg ccattctggc
    16681 agaaagtacg tccgtgggcg gcacagctta cccaaatggg cgtagcagca tgcgtatcgc
    16741 ttgcagttat cgttggcgtc cagcactata atgggcaatc tgaaacgtcc cagcagcccg
    16801 aaacgccagt atttaataca ctgccgatga tgggtaaagc cagcccggtt agtctgggag
    16861 taccttctga agcgaccgca aataatggtc aacagcagca ggtacaggag cagcgtcgtc
    16921 gcattaatgc aatgttgcag gattacgaac tgcaacgccg actccactct gaacagcttc
    16981 agtttgagca ggcacaaact cagcaagccg ctgtacaggt gccaggaatt caaactttag
    17041 gaacgcaatc gcagtaatga agcaactttg gtttgccatg tcattagtga caggtagcct
    17101 gttattctct gctaacgcct tggccactcc cgcgtccggg gcgttattac agcagatgaa
    17161 cctggccagt cagtcactga attacgagct gtcatttatc agcatcaata aacagggtgt
    17221 tgagtctctg cgttatcgac atgcacgcct cgataaccgt cctcttgcac aattgttgca
    17281 aatggatggc ccgcgccggg aagtggtaca gcgcggcaat gaaatcagct attttgaacc
    17341 gggacttgaa ccgttcacgc ttaatggcga ttacattgtt gattctctgc catcgctcat
    17401 ctataccgat ttcaaacgcc tttctcctta ctacgacttt atctctgtcg ggcgcacgcg
    17461 tattgctgat cgtctttgcg aagtcattcg tgtggttgcc cgagatggta cacgctacag
    17521 ctacatcgtg tggatggaca ccgaatcgaa attaccgatg cgggttgatc tccttgatcg
    17581 cgatggtgaa acgctggaac aatttcgcgt gattgctttt aacgtcaatc aggatatcgg
    17641 cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg ttgctttctg ttcctgttgg
    17701 tgaaaaagct aaattcagct ggacgccaac ctggctgcca cagggtttta gcgaagtttc
    17761 cagtagtcga cgtccgctac cgacgatgga caacatgcct atcgaatcac gtctctattc
    17821 cgacggatta ttcagcttct cggtaaacgt taaccgcgct acgccatcga gcaccgatca
    17881 gatgttgcgc accggacgca gaaccgtcag tacaagcgta cgtgataacg ccgaaatcac
    17941 cattgttggt gaactgccgc cgcaaacggc gaaacgcatt gccgagaata ttaagttcgg
    18001 gacagcgcaa tgatcaaaga gtgggctacc gtcgtctcct gggaaaacgg gcaggcgctg
    18061 gtcagttgtg atgttaaagc ctcatgcagc agctgcgctt cacgcgccgg ttgcggtagc
    18121 cgtgtgttga ataaacttgg cccgcaaacc acgcatacca ttgtcgtacc ctgtgatgag
    18181 ccgctagtgc cggggcaaaa agtggaattg gggatcgccg aaggcagcct tcttagctcc
    18241 gcattactgg tttatatgtc gccgctggtg ggattattcc tcatcgcttc gctatttcag
    18301 ctactctttg cttctgacgt cgcagcatta tgtggtgcga tcctcggcgg tattggtggg
    18361 ttcctgattg cgcgcggcta ttcgcgtaag tttgctgccc gggcagaatg gcaaccgatc
    18421 atcttaagcg tggcattgcc gcctggcctt gtgcgatttg aaacatcttc ggaagacgcg
    18481 agccagtgat ttcactggct ttgttgcatt ttgcataaca aatgcgagga cgtttccata
    18541 taccacaatc catacggtat gaacatttcc tcgcctcaat gttgtagtgt agaatgcggc
    18601 gtttctatta atacagacgt taagctcaga acagcgactt ctaaaagcct ggttaaccgg
    18661 gcattaaggc acaataatca tactttatga agaatatacg taacttttcg atcatagctc
    18721 acattgacca cggtaaatcg acgctgtctg accgtattat ccagatctgc ggtggcctgt
    18781 ctgaccgtga aatggaggcg caggttctcg attccatgga tcttgagcgt gagcgtggca
    18841 ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc gtctgacggc gaaacctatc
    18901 agcttaactt tatcgacacc ccgggccacg tagacttctc ctatgaagtt tctcgttcgc
    18961 tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg gcagggcgta gaagcgcaaa
    19021 ccctggcaaa ctgctacacc gccatggaaa tggatctcga agtggtgccg gtactgaaca
    19081 agattgacct gccggcagcc gatcctgaac gcgtggcgga agaaattgaa gatatcgtcg
    19141 gcatcgacgc caccgacgcg gtgcgctgtt cagcgaaaac cggcgttggc gtgcaagacg
    19201 ttctcgaacg cttggtgcgc gacattccgc cgccggaagg cgatccggaa ggcccattgc
    19261 aggcgctgat tatcgactcc tggttcgaca actacctggg cgttgtttca cttatccgta
    19321 ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt catgagcacc gagcagacct
    19381 ataacgccga tcgtctgggc atcttcacgc cgaagcagat tgaccgcact gaactgaaat
    19441 gtggcgaagt aggctggctg gtatgtgcga ttaaagatat ccacggcgct ccagtaggcg
    19501 ataccttaac actggcgcgt aatccggcag aaaaggcgct gcctggcttt aagaaagtca
    19561 aacctcaggt atatgctggt ctgttcccgg taagttccga cgactatgaa gccttccgtg
    19621 acgcgctggg taaactcagc ctgaacgatg cctcactgtt ctatgagccg gaaagctcca
    19681 gcgcgttggg ctttggtttc cgctgcggct tccttggcct gctgcacatg gagatcatcc
    19741 aggaacgtct ggaacgtgaa tacgatctgg atctgatcac cactgcgccg actgtagtgt
    19801 atgaagttga aaccacgtca agagaagtta tctacgtcga cagcccatcc aagctgccag
    19861 cggtaaataa catttacgaa ctgcgcgagc cgattgcaga gtgtcacatg ctgctgccgc
    19921 aggcatatct cggcaacgtt attacgctgt gcgtagaaaa acgcggcgtg cagaccaata
    19981 tggtttacca cggtaatcag gtggcgctga cgtacgagat cccgatggcg gaagtggtgc
    20041 tcgacttctt cgatcgcctg aaatctacct cgcgtggtta tgcgtctctg gattacaact
    20101 tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt attaatcaac ggtgaacgtg
    20161 ttgatgcatt ggcgctgatc acccaccgtg gtaattcgca aaaccgcggt cgcgagctgg
    20221 tggagaagat gaaagatctg atcccacgcc agcagtttga tatcgccatt caggcagcga
    20281 ttggtacgca catcattgcg cgctccaccg tgaaacagct gcgtaaaaac gtactggcta
    20341 aatgttatgg cggcgatatc agccgtaaga aaaagctgct gcagaagcag aaagaaggta
    20401 agaaacgcat gaagcagatc ggtaacgtcg agctgccgca ggaagcgttc ctcgccattc
    20461 tgcacgtcgg caaagacaac aaataaccct taggagttgg catggcgaat atgtttgccc
    20521 tgattctggt gattgccaca ctggtgacgg gcattttatg gtgcgtggat aaattctttt
    20581 tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc ggctgccggt gactcactgg
    20641 ataaagcaac gctgaaaaag gttgcgccga agcctggctg gctggaaacc ggtgcttctg
    20701 tttttccggt actggctatc gtattgattg tgcgttcgtt tatttatgaa ccgttccaga
    20761 tcccgtcagg ttcgatgatg ccgactctgt taatcggtga ttttattctg gtagagaagt
    20821 ttgcttatgg cattaaagat cctatctacc agaaaacgct gatcgaaacc ggtcatccga
    20881 aacgcggcga tatcgtggtc tttaaatacc cggaagatcc aaagcttgat tacatcaagc
    20941 gcgcggtggg tttaccgggc gataaagtca cttatgatcc ggtctcaaaa gagctgacga
    21001 ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc gctaccggtc acttactcca
    21061 acgtggaacc gagcgatttc gttcagacct tctcacgccg taatggtggg gaagcgacca
    21121 gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa tggaattcgt ctttccgagc
    21181 gtaaagagac actgggtgat gtgacgcacc gtattctgac agtgccgatt gcgcaggacc
    21241 aggtggggat gtattaccag cagccagggc aacaactggc aacctggatt gttccgccgg
    21301 gacaatactt catgatgggc gacaaccgcg acaacagcgc ggacagtcgt tactggggct
    21361 ttgtgcctga agcgaatctg gtcggtcggg caacggctat ctggatgagc ttcgataagc
    21421 aagaaggcga atggccgaca ggtgtgcgct taagtcgcat tggcggtatc cattaatagc
    21481 catcttcgtt cacgttgtcg ccgttatggc gacaacgtga attatttatg agataaatct
    21541 cccgtggcta acgacatccc ccgtcgttgt gtatagaata ttcccccgaa gtttaaggtt
    21601 ggcacctcca ggttgccacg gcacacgaaa cagcgttggt ccccatatac cggtaaactg
    21661 aaactgcagt gaagcagtta gccgagccat gtatatcagg tctgtttcgt gtgctgaatt
    21721 gttgacgcat ttatttattg gtatcgcatg aaccccatcg taattaatcg gcttcaacgg
    21781 aagctgggct acacttttaa tcatcaggaa ctgttgcagc aggcattaac tcatcgtagt
    21841 gccagcagta aacataacga gcgtctggaa tttttaggtg actctattct gagctacgtt
    21901 atcgccaatg cgctttatca ccgtttccct cgtgtggatg aaggcgatat gagccggatg
    21961 cgcgccacgc tggtccgtgg taatacgctg gctgaactgg cgcgcgaatt tgagttaggt
    22021 gagtgcttac gtttagggcc aggtgaactt aaaagcggtg gatttcgtcg tgagtcaatt
    22081 ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc tcgacagtga tattcaaacc
    22141 gtcgagaaat taatcctcaa ctggtatcaa actcgtctgg acgaaattag cccaggcgat
    22201 aaacaaaaag atccgaaaac gcgtctgcag gaatatttgc agggtcgcca tctgccgctg
    22261 ccaacttatc tggtagtcca ggtacgtggc gaagcgcacg atcaggaatt tactatccac
    22321 tgccaggtca gcggcctgag tgaaccggtg gttggcacag gttcaagccg tcgtaaggct
    22381 gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc tggaatgagc atcgataaaa
    22441 gttactgcgg atttattgct atcgtcggac gtccgaacgt tggcaaatcc acattgttga
    22501 acaaactgct ggggcagaag atctccatta cctcccgcaa ggcgcaaaca actcgtcacc
    22561 gcattgtggg gatccatact gaaggcgcgt atcaggcgat ctacgttgat accccaggcc
    22621 tgcatatgga agaaaaacgc gccattaacc gtctgatgaa caaagcggcg agcagctcca
    22681 ttggcgatgt tgagctggtg atttttgtcg tagaaggcac tcgctggacg ccggacgatg
    22741 aaatggtgct caacaaactg cgcgatggca aagcgccggt aatcctcgcg gtgaacaaag
    22801 tggataatgt gcaggaaaaa gccgatctgc tgccacactt acagttcctg gcaagccaga
    22861 tgaacttcct cgatatcgtg ccgatctctg ccgaaaccgg gctgaatgtc gacactattg
    22921 cagcaatcgt gcgtaagcat ctgcctgaag cgacccatca cttcccggaa gattacatca
    22981 ccgatcgctc acagcgtttt atggcgtctg aaattatccg cgaaaaactg atgcgttttc
    23041 tcggcgctga actgccgtac tccgtgaccg tggagatcga acgtttcgtc tctaacgaac
    23101 gcggcggtta tgacatcaac ggtttgattc tcgttgagcg tgaagggcag aagaagatgg
    23161 tcattggcaa caaaggggcc aagatcaaaa ccatcgggat tgaagcgcgt aaagacatgc
    23221 aggaaatgtt cgaagcgcct gttcaccttg agctgtgggt aaaagtgaaa tccggttggg
    23281 ccgacgacga acgcgcactg cgcagtctcg gttatgttga cgatctttaa gagtaactcc
    23341 gatggaaggc tggcagcgcg catttgtcct gcatagtcgc ccgtggagcg aaaccagcct
    23401 gatgctggac gtcttcacgg aggaatcggg tcgcgtgcgt ctggttgcca aaggcgcacg
    23461 ctctaaacgc tctactctga aaggtgcatt acagcctttc acccctctct tgctacgttt
    23521 tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa gctgtctcgc tggcgctgcc
    23581 attaagcggt atcacgcttt acagcggtct gtacatcaac gaacttctct cccgcgtact
    23641 ggaatacgag acgcgcttct ctgaactctt tttcgattac ttgcactgca ttcagtctct
    23701 tgccggggtc actggtacgc cagaacccgc gttgcgccgc tttgaactgg cactgctcgg
    23761 gcatctgggc tatggcgtca attttaccca ttgtgcgggt agcggcgagc cggtagatga
    23821 caccatgacg tatcgttatc gcgaagaaaa agggtttatc gcaagcgtcg ttatcgacaa
    23881 taaaacgttc accggaaggc agttaaaagc gttaaacgcg cgggaatttc ctgacgcaga
    23941 cacactgcgc gccgcgaaac gctttacccg catggcgctt aagccgtatc ttggcggtaa
    24001 accattaaag agcagggaac tgttccggca gtttatgcct aagcgaacgg tgaaaacaca
    24061 ttatgaatga tgaggattgt catggctgaa ttactgttag gcgtcaacat tgaccatatt
    24121 gcaacactgc gtaacgcgcg cggtactgct tacccggatc cggtgcaggc ggcgtttatt
    24181 gctgagcagg cgggagcgga cggcattacc gtgcatttac gtgaagaccg ccgccacatt
    24241 accgaccgcg atgtgtgcat cctgcgccag acgctggata cccgcatgaa tctggagatg
    24301 gcggtgaccg aagagatgct ggcgatcgcc gttgagacga agccacattt ttgctgtctg
    24361 gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc tggacgtcgc agggcagcgt
    24421 gaaaaaatgc gcgatgcctg caaacgtctg gcagatgccg ggatccaggt ttctctgttt
    24481 atcgacgccg atgaagagca gatcaaagct gcggcagagg ttggcgcacc gtttatcgag
    24541 atccacaccg gttgctatgc tgatgccaaa actgacgccg aacaggcgca agagttggcg
    24601 cgtatcgcca aagccgcgac ctttgccact agtctcggtc tgaaagttaa cgccggacac
    24661 ggtctgacct atcacaacgt gaaagccatt gccgccatcc ctgagatgca tgaactgaat
    24721 atcggtcatg ccattattgg tcgtgcagtg atgaccggac tgaaagatgc ggtggcagaa
    24781 atgaagcgtc tgatgctgga agcgcgtggc taatggcaat attaggttta ggtacggata
    24841 tcgtagagat cgctcgcata gaagcggtga tcgcccgatc cggtgagcgc ctggcacgcc
    24901 gcgtattaag cgataacgaa tgggaaatct ggaaaacgca ccaccagcca gtgcgttttc
    24961 tggcgaagcg ttttgctgtg aaagaagcgg cggcaaaagc atttggcaca gggatccgca
    25021 atggtctggc gtttaatcag tttgaagtat tcaatgatga gctcggcaaa ccacggctac
    25081 ggctatgggg tgaggcatta aaactggcgg aaaagctcgg cgttgtaaat atgcatgtaa
    25141 cgcttgccga tgagcgccac tatgcttgtg ccacggtaat tattgaaagt taaaataata
    25201 ccgttctctt tataaagtaa aacccccgaa ctggtaagga acgggggttt tactttttaa
    25261 ctttaatttt gtgcagcaca cgattccaac gaaaatgatt atggatcaat ttcgttgacc
    25321 tgtacaagca acttgcaatc tctgacataa aaaacgcgag cctttacgaa gaaatcgaca
    25381 ttgatgaaaa gattaacgtg accgcttttt gataagtaca ttaaaatcgg tttcgttatt
    25441 ggcaattttg ctgtgcttta caccatgcca ctgaattccc ccattgaaac gagtggtgtc
    25501 gtcaaagctc tggtgtggag tgcagcactc gattccaata actcgcacgt tcagttttgg
    25561 ggagatgtaa gggctaatct gaacggctgc attccttgtt taaggaaaaa cgaatgactg
    25621 attgccgata cctgattaaa cgggtcatca aaatcatcat tgctgtttta cagctgctcc
    25681 ttttgttctt ataacacaag gaaacgtact taaggtgcgt ccggtgaacc aatcggacgc
    25741 gccttt
//
LOCUS       DAPQZD010000058        25486 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPQZD010000058 DAPQZD010000000
VERSION     DAPQZD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.058"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            108..512
                     /gene="ybgC"
                     /locus_tag="RO454_002342"
     CDS             108..512
                     /gene="ybgC"
                     /locus_tag="RO454_002342"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDY0934169.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            509..1201
                     /gene="tolQ"
                     /locus_tag="RO454_002343"
     CDS             509..1201
                     /gene="tolQ"
                     /locus_tag="RO454_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDY0934170.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1205..1633
                     /gene="tolR"
                     /locus_tag="RO454_002344"
     CDS             1205..1633
                     /gene="tolR"
                     /locus_tag="RO454_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDY0934171.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1698..3008
                     /gene="tolA"
                     /locus_tag="RO454_002345"
     CDS             1698..3008
                     /gene="tolA"
                     /locus_tag="RO454_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDY0934172.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAAD
                     KKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNA
                     SPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLD
                     IKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            3141..4433
                     /gene="tolB"
                     /locus_tag="RO454_002346"
     CDS             3141..4433
                     /gene="tolB"
                     /locus_tag="RO454_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDY0934173.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4468..4989
                     /gene="pal"
                     /locus_tag="RO454_002347"
     CDS             4468..4989
                     /gene="pal"
                     /locus_tag="RO454_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDY0934174.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            4999..5790
                     /gene="cpoB"
                     /locus_tag="RO454_002348"
     CDS             4999..5790
                     /gene="cpoB"
                     /locus_tag="RO454_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDY0934175.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTSNAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5955..6030
                     /locus_tag="RO454_002349"
     tRNA            5955..6030
                     /locus_tag="RO454_002349"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5988..5990,aa:Lys,seq:ttt)
     gene            6066..6141
                     /locus_tag="RO454_002350"
     tRNA            6066..6141
                     /locus_tag="RO454_002350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6099..6101,aa:Val,seq:tac)
     gene            6144..6219
                     /locus_tag="RO454_002351"
     tRNA            6144..6219
                     /locus_tag="RO454_002351"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6177..6179,aa:Lys,seq:ttt)
     gene            6271..6346
                     /locus_tag="RO454_002352"
     tRNA            6271..6346
                     /locus_tag="RO454_002352"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6304..6306,aa:Val,seq:tac)
     gene            6350..6425
                     /locus_tag="RO454_002353"
     tRNA            6350..6425
                     /locus_tag="RO454_002353"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6383..6385,aa:Lys,seq:ttt)
     gene            6474..6549
                     /locus_tag="RO454_002354"
     tRNA            6474..6549
                     /locus_tag="RO454_002354"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6507..6509,aa:Lys,seq:ttt)
     gene            6583..6658
                     /locus_tag="RO454_002355"
     tRNA            6583..6658
                     /locus_tag="RO454_002355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6616..6618,aa:Lys,seq:ttt)
     gene            6936..7979
                     /gene="nadA"
                     /locus_tag="RO454_002356"
     CDS             6936..7979
                     /gene="nadA"
                     /locus_tag="RO454_002356"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDY0934176.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            8017..8736
                     /gene="pnuC"
                     /locus_tag="RO454_002357"
     CDS             8017..8736
                     /gene="pnuC"
                     /locus_tag="RO454_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDY0934177.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(8733..9674)
                     /gene="zitB"
                     /locus_tag="RO454_002358"
     CDS             complement(8733..9674)
                     /gene="zitB"
                     /locus_tag="RO454_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDY0934178.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(9788..10168)
                     /locus_tag="RO454_002359"
     CDS             complement(9788..10168)
                     /locus_tag="RO454_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDY0934179.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            10484..11536
                     /gene="aroG"
                     /locus_tag="RO454_002360"
     CDS             10484..11536
                     /gene="aroG"
                     /locus_tag="RO454_002360"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDY0934180.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(11703..12455)
                     /gene="gpmA"
                     /locus_tag="RO454_002361"
     CDS             complement(11703..12455)
                     /gene="gpmA"
                     /locus_tag="RO454_002361"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDY0934181.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12658..13698)
                     /gene="galM"
                     /locus_tag="RO454_002362"
     CDS             complement(12658..13698)
                     /gene="galM"
                     /locus_tag="RO454_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDY0934182.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(13692..14840)
                     /gene="galK"
                     /locus_tag="RO454_002363"
     CDS             complement(13692..14840)
                     /gene="galK"
                     /locus_tag="RO454_002363"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDY0934183.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(14844..15890)
                     /gene="galT"
                     /locus_tag="RO454_002364"
     CDS             complement(14844..15890)
                     /gene="galT"
                     /locus_tag="RO454_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDY0934184.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(15900..16916)
                     /gene="galE"
                     /locus_tag="RO454_002365"
     CDS             complement(15900..16916)
                     /gene="galE"
                     /locus_tag="RO454_002365"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDY0934185.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(17179..18651)
                     /gene="modF"
                     /locus_tag="RO454_002366"
     CDS             complement(17179..18651)
                     /gene="modF"
                     /locus_tag="RO454_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDY0934186.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(18719..19507)
                     /gene="modE"
                     /locus_tag="RO454_002367"
     CDS             complement(18719..19507)
                     /gene="modE"
                     /locus_tag="RO454_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDY0934187.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            19636..19785
                     /gene="acrZ"
                     /locus_tag="RO454_002368"
     CDS             19636..19785
                     /gene="acrZ"
                     /locus_tag="RO454_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDY0934188.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            19952..20725
                     /gene="modA"
                     /locus_tag="RO454_002369"
     CDS             19952..20725
                     /gene="modA"
                     /locus_tag="RO454_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0934189.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            20725..21414
                     /gene="modB"
                     /locus_tag="RO454_002370"
     CDS             20725..21414
                     /gene="modB"
                     /locus_tag="RO454_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDY0934190.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            21417..22475
                     /gene="modC"
                     /locus_tag="RO454_002371"
     CDS             21417..22475
                     /gene="modC"
                     /locus_tag="RO454_002371"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDY0934191.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(22476..23294)
                     /gene="ybhA"
                     /locus_tag="RO454_002372"
     CDS             complement(22476..23294)
                     /gene="ybhA"
                     /locus_tag="RO454_002372"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDY0934192.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            23449..24444
                     /gene="pgl"
                     /locus_tag="RO454_002373"
     CDS             23449..24444
                     /gene="pgl"
                     /locus_tag="RO454_002373"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDY0934193.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(24485..25438)
                     /locus_tag="RO454_002374"
     CDS             complement(24485..25438)
                     /locus_tag="RO454_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0934194.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT         6231 a         6251 c         6749 g         6255 t
ORIGIN      
        1 taaattttta cgctccctta acttgccctc attcccaaac ctcaatcgcg cgcgtatagt
       61 agcagcgttt aaaagctcta acttttgttg cattaccggg atgtaaagtg aatacaacgc
      121 tgtttcgatg gccggttcgc gtctactatg aagataccga tgccggtggt gtggtgtacc
      181 acgccagtta cgtcgctttt tatgaaagag cacgcacaga gatgctgcgt catcatcatt
      241 tcagtcaaca ggcgctgatg gctgaacgcg ttgcctttgt ggtacgtaaa atgacggtgg
      301 aatattacgc acctgcgcgg ctcgacgata tgctcgaaat acagactgaa ataacatcaa
      361 tgcgtggcac ctctttggtt ttcacgcaac gtattgtcaa cgccgagaat actttgttga
      421 atgaagcaga ggttctggtt gtttgcgttg acccactcaa aatgaagcct cgtgcgcttc
      481 ccaagtctat tgtcgcggag tttaagcagt gactgacatg aatatccttg atttgttcct
      541 gaaggctagc cttctggtta aacttatcat gttgattttg attggttttt caatcgcatc
      601 ctgggccatt attatccagc ggacccgtat tcttaacgca gcggcgcgcg aagccgaagc
      661 gtttgaagat aaattctggt ctggaatcga actctctcgc ctctatcaag agagccaggg
      721 gaaacgggat aatctgagcg gttcggaaca aatcttttac agcggcttca aagagtttgt
      781 gcgcctgcat cgtgccaata gccatgcgcc ggaagccgta gtggaagggg cgtcgcgtgc
      841 gatgcgtatt tccatgaatc gtgaacttga aaatctggaa acgcacattc cgttcctcgg
      901 tacggttggc tccatcagcc cgtatattgg tctgtttggt acggtctggg ggatcatgca
      961 cgcctttatc gccctcgggg cggtaaaaca agcaacactg caaatggttg cgcccggtat
     1021 cgcagaagcg ttgattgcga cggcaattgg tttgttcgcc gcaattccgg cggtaatggc
     1081 ttataaccgt ctcaaccagc gcgtaaacaa actggaactg aattacgaca actttatgga
     1141 agagtttacc gcgattctgc accgccaggc gtttaccgtt agcgagagca acaaggggta
     1201 agccatggcc agagcgcgtg gacgaggtcg tcgcgatctc aagtccgaaa tcaacattgt
     1261 accgttgctg gacgtactgc tggtgctgtt gctgatcttt atggcgacag cgcccatcat
     1321 cacccagagc gtggaggtcg atctgccaga cgctactgaa tcacaggcgg tgagcagtaa
     1381 cgataatccg ccagtgattg ttgaagtgtc tggtattggt cagtacaccg tggtggttga
     1441 gaaagatcgc ctggagcgtt taccaccaga gcaggtggtg gcggaagtgt ccagccgttt
     1501 caaggccaac ccgaaaacgg tctttctgat cggtggcgca aaagatgtgc cttacgatga
     1561 aataattaaa gcactgaact tgttacatag tgcgggtgtg aaatcggttg gtttaatgac
     1621 gcagcctatc taaacatctg cgtttccctt gcttgaaaga gagcgggtaa caggcgaaca
     1681 gtttttggaa accgagagtg tcaaaggcaa ccgaacaaaa cgacaagctc aagcgggcga
     1741 taattatttc agcagtgctg catgtcatct tatttgcggc gctgatctgg agttcgttcg
     1801 atgagaatat agaagcttca gccggaggcg gcggtggttc gtccatcgac gctgtcatgg
     1861 ttgattcagg tgcggtagtt gagcagtaca aacgcatgca aagccaggaa tcaagcgcga
     1921 agcgttctga tgagcagcgc aagatgaaag agcagcaggc tgctgaagaa ctgcgtgaga
     1981 aacaagcggc tgaacaggaa cgcctgaagc aacttgagaa agagcggtta gcggctcagg
     2041 agcagaaaaa gcaggctgaa gaagccgcaa aacaggccga gttaaagcag aagcaagcgg
     2101 aagaggcggc agcgaaagcg gcggcagatg ctaaagcgaa ggccgaagcg gatgctaaag
     2161 ctgcggaaga agcagcgaag aaagcggcgg cagacgcgaa gaaaaaagca gaagcagaag
     2221 ccgccaaagc cgcagtcgaa gcgcagaaaa aagccgaggc tgctgctgcg gcgctgaaga
     2281 agaaagctga agcggcagaa gcagctgcag ctgaagcaag aaagaaagcg gcaaccgaag
     2341 ctgctgaaaa agccaaggca gaagctgaga agaaagcggc agcagagaaa gcggcagccg
     2401 acaaaaaagc agcagaaaaa gcggctgctg aaaaggctgc agctgataag aaagcggcag
     2461 cagagaaagc ggcagccgac aaaaaagcag cagaaaaagc ggctgctgaa aaggcagcag
     2521 ctgataagaa agcagcggca gaaaaagccg ccgcagacaa aaaagcggca gcggcaaaag
     2581 cagcagctga aaaagccgct gcagcaaaag ctgccgctga ggcagatgat attttcggtg
     2641 agctaagctc tggtaagaat gcaccgaaaa cggggggagg ggcgaaaggg aacaatgctt
     2701 cgcctgccgg gagtggtaat actaaaaaca atggcgcatc aggggccgat atcaataact
     2761 atgccgggca gattaaatct gctatcgaaa gtaagttcta tgacgcatcg tcctatgcag
     2821 gcaaaacctg tacgctgcgc ataaaactgg cacccgatgg tatgttactg gatatcaaac
     2881 ctgaaggtgg cgatcccgca ctttgtcaag ctgcgttggc agcagctaaa cttgcgaaga
     2941 tcccgaaacc accaagccag gcagtatatg aagtgttcaa aaacgcgcca ttggacttca
     3001 aaccgtaatc gcgatgttga ctgttccgac ggtcaacatc aggcaccggt tgccacgggg
     3061 ttctggtagt tttgtgtatt ttagtttgtt aacattctgc taaattatcg tgggccatcg
     3121 gtccagataa gggagatatg atgaagcagg cattacgagt agcatttggt tttctcatac
     3181 tgtgggcatc agttctgcat gctgaagtcc gcattgtgat cgacagcggt gtagattccg
     3241 gtcgtcctat tggtgttgtt cctttccagt gggcggggcc tggtgcggca cctgaagata
     3301 ttggcggcat cgttgctgct gacttgcgta acagcggtaa atttaatccg ttagatcgtg
     3361 ctcgtctgcc acagcagccg ggtagtgcgc aggaagtaca accagctgca tggtccgcgc
     3421 tgggcattga cgctgtggtt gtcggtcagg tcactccgaa tccggatggt tcttacaatg
     3481 ttgcttatca acttgttgac actggcggcg caccgggtac tgtacttgct cagaactcgt
     3541 acaaagtgaa caagcagtgg ctgcgttatg ctggtcatac cgccagtgac gaagtatttg
     3601 aaaaactgac cggtattaaa ggtgcgttcc gtacccgtat tgcctacgtt gttcagacta
     3661 acggcggtca gttcccgtat gaactgcgcg tatctgacta tgacggttac aaccagtttg
     3721 tcgttcaccg ttcaccgcaa ccgctgatgt ctccggcgtg gtcaccagac ggttctaaac
     3781 tggcttatgt gaccttcgaa agcggtcgtt ccgcgctggt tattcagacg ctggcaaatg
     3841 gcgctgtacg tcaggtggct tcattcccgc gtcacaacgg tgcacctgca ttctcgccag
     3901 acggcagcaa actggcattc gccttgtcga aaaccggtag cctgaacctg tacgtaatgg
     3961 atttggcttc tggtcagatc cgccaggtaa ctgatggtcg cagtaacaat accgaaccga
     4021 cctggttccc ggacagccag aacctggcat ttacttctga ccaggccggt cgtccgcagg
     4081 tttataaagt gaatatcaac ggcggtgcgc cacaacgtat tacctgggaa ggttcgcaga
     4141 accaggatgc ggatgtcagc agcgacggta aatttatggt aatggtcagc tccaacggtg
     4201 ggcagcagca cattgccaaa caagatctgg caacgggagg cgtacaagtt ctgtcgtcca
     4261 cgttcctgga tgaaacgcca agtctggcac ctaacggcac tatggtaatc tacagctctt
     4321 ctcaggggat gggatccgtg ctgaatttgg tttctacaga tgggcgtttc aaagcgcgtc
     4381 ttccggcaac tgatggacag gtcaaattcc ctgcctggtc gccgtatctg tgataataat
     4441 taattgaata gtaaaggaat cattgaaatg caactgaaca aagtgctgaa agggctgatg
     4501 attgctctgc ctgttatggc aattgcggca tgttcttcca acaagaacgc cagcaatgac
     4561 ggcagcgaag gcatgctggg tgccggcact ggtatggatg cgaacggcgg caacggcaac
     4621 atgtcttccg aagagcaggc tcgtctgcaa atgcaacagc tgcagcagaa caacatcgtt
     4681 tacttcgatc tggacaagta cgatatccgt tctgacttcg ctcaaatgct ggatgcacac
     4741 gcaaacttcc tgcgtagcaa cccgtcttac aaagtcaccg tagaaggtca cgcggacgaa
     4801 cgtggtactc cggaatacaa catctccctg ggtgaacgtc gtgcgaacgc tgttaagatg
     4861 tatctgcagg gtaaaggcgt ttctgctgac cagatctcca tcgtttctta cggtaaagaa
     4921 aaacctgcag tactgggtca tgacgaagcg gcgtatgcca aaaaccgtcg tgcggtactg
     4981 gtttactaag agaattgcat gagcagtaac ttcagacatc aactattgag tctgtcgtta
     5041 ctggttggta tagcggcccc ctgggccgct tttgctcagg caccaatcag tagtgtcggc
     5101 tcaggctcgg tcgaagaccg cgtcactcaa cttgagcgta tttctaatgc tcacagccag
     5161 cttttaaccc aactccagca acaactctct gataatcaat ccgatattga ttccctgcgt
     5221 ggtcagattc aggaaaatca gtatcaactg aatcaggtcg tggagcggca aaagcagatc
     5281 ctgttgcaga tcgacagcct cagcagcggt ggtgcagcgg cgcaatcaac cagcggcgat
     5341 caaagcggtg tggtggcatc aacgacgccg acagctgatg ctggtacttc gaatgctggc
     5401 gcgccggtga aaagcggtga tgcaaacacg gattacaatg cagctattgc gctggtgcag
     5461 gataaatccc gccaggatga cgcaatggtg gcatttcaga atttcatcaa aaattaccct
     5521 gattcaactt acctgccaaa cgccaattat tggctgggtc agttaaacta caacaagggt
     5581 aaaaaagatg atgcggcgta ttattttgct tcggtagtga aaaactatcc gaagtcaccg
     5641 aaggctgcag atgcgatgtt taaagtcggc gtcatcatgc aggacaaagg tgacaccgca
     5701 aaagcgaaag ccgtatacca gcaggttatc agtaaatacc ctggtactga tggcgctaaa
     5761 caggcgcaaa aacgtctgaa cgcgatgtaa tgcataacac acgaccagaa gtcgcattat
     5821 ttctggtcgt gtcgtgcgaa tcataagcag ttgagtgatc tacatcgaaa tttttgttgc
     5881 gctcaagtct gaaatcagta atatatgccg ccgttgccac gggatatcaa acaaaccgaa
     5941 agcaacgaaa aagtgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
     6001 tcgcaggttc gaatcctgca cgacccacca ctaacatagt tagttgtagt atccagcgta
     6061 gtatcgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
     6121 cgatcccgtc atcacccacc accgggtcgt tagctcagtt ggtagagcag ttgactttta
     6181 atcaattggt cgcaggttcg aatcctgcac gacccaccac tgattacggt gggttcggtg
     6241 gaagtagttt gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc
     6301 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccactcg ggtcgttagc
     6361 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
     6421 caccaatttt aaacattggt tccgagtaga aatctttcag gtaacacccg tatgggtcgt
     6481 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
     6541 gacccaccat cctgaatgat taaagcagca taatcccgca aggggtcgtt agctcagttg
     6601 gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccaat
     6661 gcaaaaaagc gccctaaagg cgtttttttg ctattcaggc atctctcaat ttcaattctt
     6721 gaccctgatg acatactccg cgtttgctgc gtaaacaact ctacgtcgga ggaataagac
     6781 gcaaagtgtg gcattcggca tctcatacgt aattcgagtg acttttcccc accattcgac
     6841 tatcttgttt agtatataaa acaaattacg ccgataatag cgaatatcac gcttaagtcg
     6901 gttttaacgt taagcctgta gaacgagatg gtaagatgag cgtgatgttt gatccagaca
     6961 cggcgattta tcctttcccc ccgaagccga cgccgttaag cattgatgaa aaagcgaatt
     7021 accgcgagaa gataaaacgt ctactaaaag aacgtaatgc ggtgatggtt gcccactact
     7081 ataccgatcc cgaaattcaa caactggcag aagaaaccgg tggctgtatt tctgattctc
     7141 tggaaatggc gcgcttcggt gcaaagcatc ccgcttctac gttgctagtc gctggggtga
     7201 gatttatggg agaaaccgcc aaaattctca gtccggaaaa aacaattctg atgccgacac
     7261 ttcaggctga atgttcactg gatctcggct gccctgttga agaatttaac gcattttgcg
     7321 atgcccatcc cgatcgtact gtcgtcgtct acgccaacac ttctgctgcg gtaaaagcgc
     7381 gcgcagattg ggtggtaact tcaagcattg ccgtcgaact tattgatcat ctggatagtt
     7441 tgggtgaaaa aatcatctgg gcacctgaca aacatctggg gcgttacgtg caaaaacaga
     7501 cgggcgcaga cattttatgc tggcagggtg cctgcattgt gcatgatgag tttaagactc
     7561 aggcgttaac ccgcttgcaa gaagaatacc cggatgctgc catactggtg catccagaat
     7621 caccgcaagc tattgtagag atggcggatg cggtcggttc caccagtcaa ctgatcgctg
     7681 ctgcgaaaac attgcaacat cagcggctta tcgtggcaac cgatcggggc attttctaca
     7741 aaatgcagca ggcggtgcca gataaagagt tactggaagc accaaccgcg ggtgagggcg
     7801 caacctgtcg cagctgcgcg cattgtccgt ggatggccat gaatggcctt caggccatcg
     7861 cagaggcatt agaactggaa ggaagcaatc acgaggttta tgttgatgaa aggctgcgag
     7921 agagggcgct ggtgccgctc aatcgtatgc tggattttgc ggctacacta cgtggataat
     7981 tgataataag gcgtcacatt acgctttggg ggaacgatgg atttttttag tgtgcagaat
     8041 atcctggtac atataccaat aggggcaggc ggttatgatc tctcatggat cgaagcggta
     8101 ggcacgatcg ccgggttgct gtgtattggc cttgccagtc tggagaagat cagtaactac
     8161 ttctttggcc tgatcaacgt caccttgttt ggcattattt tctttcagat tcagctgtat
     8221 gccagcctgt tattacaggt gtttttcttt gccgcgaata tttacggctg gtatgcgtgg
     8281 tcgcgacaaa ccagtcagaa cgaggcggag ttgaaaattc gctggttgcc attgccgaag
     8341 gcgctaagct ggctggcggt ttgcgttgtt tcgattggtc taatgacggt atttatcaat
     8401 cctgtgtttg catttttgac ccgcgtggca gtcatgatca tgcaagcatt aggattacag
     8461 gttgcgatgc ctgaactaca gccggacgct ttcccgttct gggattcatg catgatggtg
     8521 ttatctatcg tggcaatgat tctgatgaca cgtaagtatg tggaaaactg gctgttgtgg
     8581 gtgattatta acgtgattag cgtcgttatt tttgcacttc agggcgttta cgccatgtct
     8641 ctggagtaca tcatcctgac ctttattgcc ctcaacggca gccggatgtg gatcaaaagc
     8701 gcacgcgaaa gaggttcacg tgcgctgtcc cattaatgat gatgatgtga atggccagac
     8761 acccctctat tcaggtggca gtctggccca tgacaaggtt gatattccat ctgaatggtg
     8821 gcgtgctcaa tctgatagtg atccatcagg tagtgttgga tctgatccaa caaggcatcg
     8881 tgatcgtgtg gcgggatcac ctgcacatgc agcgtcatca ccggcttctc gcctaccatc
     8941 catacatgca catggtgcac attgcggact tccgggattt cccggcacaa gcgacgcttc
     9001 agttcagcga tatccagcga taccggtgca ccttcaagta attcattcac actatctttc
     9061 aacagtcgcc acgcgctacg cagaaccaga agcgacacca gtattgagag aatggggtca
     9121 gcaggtgtcc agccggtcca gataataatc aacgcggcaa taatagcccc aaccgaaccc
     9181 agcaggtccc cgagcacatg cagtgccgct gctcgcacgt tgaggttttt ctcttcgctg
     9241 ccgtgatgaa gtaaccagaa agaaagtata tttgccagca gcccagccac ggcaattgcc
     9301 atcatcatgc cgccctcgac cggacgcgga gtgcggaagc gttctatcgc ctcccagaca
     9361 atcaaaatag taatcaccac cagggcgatg gcattcacaa acgccgcgag cgtggtcaat
     9421 cttagccaac cgaaagtgtg gcgaatggtg ggaggacgac gggaaaattg cactgcgagt
     9481 agggcgaaaa gcagggcggc agtatcggtc aacatatgac ccgcatcggc cagcaatgcc
     9541 agagaaccgg aaagaaaacc accaatgact tctacgagca taaacccggc agtcacgccg
     9601 aaagcataca acaagcgacg agcattatta tcttctggca ggtgtgagga agtgtgtgag
     9661 tgtgagtgcg ccatgacgag tcatccctta attattctca cttaacttta tgacatcata
     9721 ctgcttttag aagggaaaaa ttaaaaggga gagactacgc tctcccatta ttggctattt
     9781 cgcagggtta ctgcgtggta ccgtcggttt tggtatcgac atcattgttg atgccatcac
     9841 cggtttggac ttttttattg atatccgggc agcgaccatc tttgcacatg gtgttcttgt
     9901 gctcttcatc tttggtcatg ccgtcattgt tcattgaaga accatccgga tgcagcattg
     9961 tgccgccaga accggtattt accccgttat tgtcgacgtt atttggcgcg acattttcac
    10021 gggcatcagg ggctacctgg cccgcatcag cggcggcgtt tgcctggccg ttattagttt
    10081 gcgctccgct atcggcagcc agtgcggcac cgctggcaag gcttagagtg gcagtcaaaa
    10141 atagtgtggc cagttttgtc attttcatag gatgctcctg ttatggtcgt tatgtcggat
    10201 aacctcttcc aacagtgcat ttgcaggtga atataaggca atggtttaag atttcagcca
    10261 ggcaaagata cccagcagag aatcttgaaa taattaacaa acaaaggagt tacagttaga
    10321 aattgtagga gagatctcgt ttttcgcgac aatctggcgt ttttcttgct aattccagga
    10381 ttaatccgtt catagtgtaa aaccccgttt acacattctg acggaagata tagattggaa
    10441 gtattgcatt cactaagata agtatggcaa cactggaaca gacatgaatt atcagaacga
    10501 cgatttacgc atcaaagaaa tcaaagagtt acttcctcct gtcgcattgc tggaaaaatt
    10561 ccccgctact gaaaatgccg cgaatacggt cgcccatgcc cgaaaagcga tccataagat
    10621 cctgaaaggt aatgatgatc gcctgttggt ggtgattggc ccatgctcaa ttcatgatcc
    10681 tgtcgcggct aaagagtatg ccactcgctt gctggcgctg cgtgaagagc tgaaagatga
    10741 gctggaaatc gtaatgcgcg tctattttga aaagccgcgt accacggtgg gctggaaagg
    10801 gctgattaac gatccgcata tggataacag cttccagatc aacgacggtc tgcgtattgc
    10861 ccgtaaattg ttgcttgata ttaacgacag cggtctgcca gcggcgggtg agtttctcga
    10921 tatgatcacc ccacaatatc tcgctgacct gatgagctgg ggcgcaattg gcgcacgtac
    10981 tacggaatcg caggtgcacc gcgaactggc ttcagggctt tcttgcccgg taggcttcaa
    11041 aaatggcact gatggtacga ttaaagtggc tatcgatgcc attaatgccg ccggtgcgcc
    11101 gcactgcttc ctttcagtga ccaaatgggg tcattcggcg attgtgaata ccagcggtaa
    11161 cggcgattgc catatcattc tgcgcggcgg taaagagcct aactacagcg cgaagcacgt
    11221 tgctgaagtg aaagaagggc tgaacaaagc aggcctgcca gcacaggtga tgatcgattt
    11281 cagccatgct aactcgtcta aacaattcaa aaagcagatg gatgtttgtg ctgacgtttg
    11341 ccagcagatt gccggtggcg aaaaggccat tattggcgtg atggtggaaa gccatctggt
    11401 ggaaggcaat cagagcctcg agagcgggga accgctggcc tacggtaaga gcatcaccga
    11461 tgcctgcatt ggctgggatg ataccgatgc tctgttacgt caactggcga atgcagtaaa
    11521 agcgcgtcgc gggtaaagtt taattgtcgg atgcgctgtc agagtggcgt atccgattaa
    11581 tcaccgtagg cctgataaaa cgcgcagcgt cgcatcaggc aatgcgctcc attggtagca
    11641 acgaaaaata aaaaagccga ctcattttgc agtcggcttt ctcattttta agcgaatgac
    11701 gtttacttcg ctttaccctg gttagcaacc gccgctgctt tcgccgcgat ctcgtcagca
    11761 ttacccagat aatagcgttt cagcggtttg aagttctcgt caaactcata aaccagcggc
    11821 acgccggtcg ggatattcag ctcaagaatc tcttcttcgc tcatgttatc gagatatttc
    11881 accagcgcac gtaacgagtt accgtgtgcg gcgatgatga cgcgctcacc gctcttcata
    11941 cgcggcagaa tggtttcatt ccagtaaggg atcacgcggt caatggtcag cgccaggctt
    12001 tccgtcagtg gcagctcttt ctcgctcagt ttcgcgtaac gcggatcgtg acccggataa
    12061 cgctcatcat ctttagtcag ttccggcgga gtcaccgcaa aaccacgacg ccactgtttc
    12121 acctgctcgt cgccatactt ttcagcggtt tccgctttgt tcagaccctg caatgcaccg
    12181 tagtgacgtt cgttcagttt ccaggatttc tcaacgggca gccatgcctg atccagttcg
    12241 tccagcacat tccacagggt atgaatagcg cgtttcagca cagaggtgta agcaaagtca
    12301 aagctgtaac cttcctcttt cagcagctta cctgctgctt ttgcttcgct tacgcctttc
    12361 tcagacagat ccacgtcgta ccaaccggtg aaacggtttt ctttgttcca ctgactttcg
    12421 ccatgacgaa ccagaaccag cttagttaca gccatatact tactcctcaa atcatctttt
    12481 aatgataata attctcatta tattgccgtg acgaagcaac agcaatgctt acgcataacc
    12541 atagcgaaaa tagtgtcgca gtgtaaggtt gttgtgaata ttgagttgca aatatgtcgg
    12601 tgtttgctgg tgatttgaac aatatgagga taaagccctc atgacgaggg cttaacatta
    12661 ctgagcaata aactgatatt ccgtcaggct ggaatactct tcgccaggac gcaggaagca
    12721 gtccggttgc ggccattcag ggtggttcgg gctgtccggc agaaattcgc tttccagagc
    12781 cagcccttgc cagtcagcgt aaggttcggt tccccgcgac ggtgtgccgc cgaggaagtt
    12841 gccggagtag aattgcagag ccggagcggt ggtgtagacc ttcagctgca atttttcatc
    12901 tgctgaccag acatgcgccg ccactttctt gccatcgcct ttggcctgta acaagaacgc
    12961 gtgatcgtaa cctttcactt tgcgctgatc gtcgtcggca agaaactcac tggcgatgat
    13021 tttggcgttg cggaaatcaa aagaggttcc ggcgacagat ttcaggccgt cgtgcggaat
    13081 gccgccttca tcaaccggca gatattcttc cgccagaatc tgcaacttat gattgcgcac
    13141 gtcagactgc tcgccgtcaa gattgaaata gacgtgatta gtcatattca ccgggcaagg
    13201 tttatcaact gtggcgcgat aagtaatgga gatacggtta tcgtcggtca gacgatattg
    13261 caccgtcgcg ccgagattac ccgggaagcc ctgatcgcca tcatctgaac tcagggcaaa
    13321 cagcacctga cgatcgttct ggttcacaat ctgccagcga cgtttgtcga acccttccgg
    13381 gccgccatgc aattggttga cgccctgact tggtgaaagc gtcacggttt caccgtcaaa
    13441 ggtataacgg ctattggcga tgcggttggc ataacgacca atagaggccc ccagaaatgc
    13501 ggcctgatcc tgatagcatt ccgggctggc gcagccaagc agcgcctcgc ggacgctgcc
    13561 atcggaaagc ggaatacggg cggaaagtaa agtcgcaccc cagtccatca gcgtgactac
    13621 catccctgcg ttgttacgca aagttaacag tcggtacggc tgaccatcgg gtgccagtgc
    13681 gggagtttcg ttcagcactg tcctgctcct tgtgatggtt tacaaacgta aaaagtctct
    13741 ttaatacctg tttttgcttc atattgttca gcgacagctt gctgtacggc aggcaccagt
    13801 tcttccggga tcaacgcgac gatacagcca ccaaatccgc cgccggtcat gcgtacgcca
    13861 cctttgtcgc caatcacagc tttgacgatt tctaccagag tgtcaatttg cggtacggtg
    13921 atttcgaaat catcgcgcat agaggcatga gactccgcca tcaactcgcc catacgtttc
    13981 aggtcgcctt gctccagcgc gctggcagct tcaacggtgc gggcgttttc agtcaggatg
    14041 tgacgcacgc gtttcgcgac gatcgggtcc agttcatgtg caacagcgtt gaactcttca
    14101 atggtgacat cgcgcagagc tggctgctgg aagaaacgcg caccggtttc gcactgttca
    14161 cgacgggtgt tgtattcgct gccaaccagg gtacgtttga agttactgtt gatgatgacg
    14221 acagccacgc ctttcggcat ggaaacagct ttggtcccca gtgagcggca atcgatcagc
    14281 aaggcatgat ctttcttgcc gagcgcggaa atcagttgat ccatgattcc acagttacag
    14341 cctacaaact ggttttctgc ttcctgaccg ttaagcgcga tttgtgcgcc gtccagcggc
    14401 agatgataaa gctgctgcaa tacggttccg accgcgactt ccagtgaagc ggaagaactt
    14461 aaccctgcac cctgcggcac attgccgctg atcaccatgt ccacaccgcc gaagctgttg
    14521 ttacgcagtt gcagatgttt caccacgcca cgaacgtagt tcgcccattg atagttttca
    14581 tgcgcgacaa tgggcgcatc gagggaaaac tcgtcgagct gattttcata atcggctgcc
    14641 attacgcgaa ctttacggtc atcgcgtggc gcacagctga tcacggtttg ataatcaatc
    14701 gcgcagggca gaacgaaacc gtcgttgtag tcggtgtgtt caccaatcaa attcacgcgg
    14761 ccaggcgcct gaatggtgtg agtggcaggg tagccaaatg cgttggcaaa cagagattgt
    14821 gttttttctt tcagactcat ttcttacact ccggattcgc gaaaatggat atcgctgact
    14881 gcgcgcaaac gctctgcggc ctgttctgcg gtcaggtctc gctgggtttc tgccagcatt
    14941 tcataaccaa ccataaattt acgtacggtg gcagagcgca acagaggcgg ataaaagtgc
    15001 gcgtgcagct gccagtgttg attctcttcg ccattaaatg gcgcgccgtg ccagcccata
    15061 gagtagggga aggagcactg gaagaggttg tcataacgac tggtcagctt tttcaacgcc
    15121 agagccaaat cgctgcgctg ggcgtcggtc aaatcggtga tccgtaaaac gtgggctttg
    15181 ggcagcagta gcgtttcgaa cggccaggca gcccagtaag gcacgacggc taaccagtgt
    15241 tcggtttcga caacggtacg gctaccgtct gccagctcgc gctgaacata atccaccagc
    15301 attggtgatt tctgcccggc gaaatattct ttttgcagac ggtcttcgcg ctcagcttcg
    15361 ttaggcagga agctatttgc ccaaatctgt ccgtgcggat gcgggttaga gcagcccatc
    15421 gccgcgcctt tgttttcaaa gacctgcacc cacgggtatg ttttccccag ttctgcggtt
    15481 tgctcctgcc aggttttgac gatttccgtc aatgccgcaa cgctcagttc tggcagcgtt
    15541 ttactgtgat ccggtgaaaa gcagatcacc cggctggtac cgcgcgcgct ctggcaacgc
    15601 atcaaaggat cgttgctttc tggcgcatct ggcgtgtcag acatcaaggc cgcaaagtca
    15661 ttagtgaaaa cgtaagtccc ggtgtaatcg gggtttttat cgcctgtcac ccgcacatta
    15721 cctgcgcaga ggaagcaatc tggatcgtgc gcaggtaaca cctgtttggc tggcgtttcc
    15781 tgcgccccct gccaggggcg cttagcgcgg tgcggtgaaa ccagaatcca ttgcccggtg
    15841 agcgggttgt agcggcgatg tggatgatca acgggattaa attgcgtcat ggtcgttcct
    15901 taatcgggat atccctgtgg atggcgtgac tgccagtgcc aggtgtcctg cgccatttca
    15961 tcgagtgtgc gcgttacgcg ccagttcagt tcacggtcgg ctttgctggc gtccgcccag
    16021 taggccggaa ggtcgcccgc gcgacgtggt gcaaaatgat agttaaccgg tttgccgcag
    16081 gctttactga aggcattaac tacgtccagc acgctgctgc ctacgcctgc gccgaggttg
    16141 tagatgtgta cgcctggctt gttcgccagt ttttccatcg ccacgacgtg accatctgcc
    16201 agatccatta cgtggatgta atcgcgcacg ccagtgccgt cttcggtcgg ataatcgtta
    16261 ccaaaaatcg ccagtgagtc gcgacggcct acagcaacct gggcaatgta cggcatcagg
    16321 ttattcggaa tgccttgcgg atcttcgccc atatcacccg acggatgcgc gccaaccggg
    16381 ttgaagtagc gcagcagggc aatgctccag tccggttggg ctttttgcag atcggtaagg
    16441 atctgttcca ccatcagctt gcttttgccg taaggacttt gcggtgtgcc ggtcgggaag
    16501 ctttcaacat atggaatttt gggctgatcg ccataaacgg tggcggagga gctaaagata
    16561 aagtttttga cgttagcggc gcgcatggcg ctaatcaggc gcagagtacc gttgacattg
    16621 ttgtcgtaat attcaagcgg tttttgtacc gattcgccaa cggctttcag cccggcgaag
    16681 tggatcacgg tgtcgatagc gtgatcgtgc aggatctcgg tcatcaacgc ttcgttacgg
    16741 atatcgcctt caacaaacgt tggatgtttg ccgcctaaac gctcgataac aggcagtacg
    16801 ctgcgcttac tgttacagag gttatcaaga atgatgacat catgaccgtt ttgcagtaat
    16861 tgcacacagg tatgacttcc aatgtaaccg ctaccaccgg taaccagaac tctcataatt
    16921 cgctccatta ggcttatggt atgaaataac catagcataa caaagatgcg aaaagtgtga
    16981 catggaataa attagtggaa tcgtttacac aggaatttag ccgtttttta tgcgcgatta
    17041 agtgattata aaacagcggc tttatgaatg attgcgcttt ttatctgaaa aaaaagacgc
    17101 ggtttcatgc ctggatgcgt caaaccgttg gccggataac acgtttacgc cgcctccggc
    17161 acggtcagca ttaccgactc aatttatttt tgtcagcaca tagcgataga gtccaccgtc
    17221 cggcacgaac tcaagacgat gggtaataca ggcaggcgca tcttcagcgt ggtgcgaaac
    17281 aaacaacaat tgcgtttcac cttcgctaat cagcacatca acaaaacggc ggataagctg
    17341 gcgattgagc ggatcaagcc cctgtagtgg ttcatcgaga ataagcaacg tcggatgttt
    17401 caccagtgcg cggacaatca gcgccagacg ctgctgtccc caggaaagac tatggaaagg
    17461 agcgtcagcc gtgcgtttat caatgccgag aatatccagc cactgctgca ccagtttttg
    17521 ctggcgatcc gaaacggcct gataaatgcc aatcgaatca aaatagccag aaagaatcac
    17581 attacgcaca gtagtgctga cccggtaatc cagatgcaaa ctactgctga cgtaaccgat
    17641 atgctttttg atatcccaga tggtttcgcc gctgccgcga cgtcgtccga aaagcgtcaa
    17701 atcgttgctg taaccttgcg gatgatcgcc agtaatcagg cttaataacg tcgattttcc
    17761 cgcgccattt ggcccgacaa tttgccagtg ttcgcctgga ttcacctgcc agctaaggtt
    17821 attaaggatg gggcgttcgt tataagaaac cacgccattg ttcagcacaa tgcgcggttc
    17881 gttggcgggt aaggcgtgac gtgctgaagg ttcatccggc tccggcattt gcacaccttc
    17941 aagcagttcg ctatgcgcca gttgcgcaac gagtgcttgt tggagcagtt cttctttagc
    18001 gccagtttcc gctaacgtgc aatccgccag cacgccagca aactggacaa attccgggat
    18061 ctcatcgaag cgattgagca ccagtaccag agtaataccg gactgatgta acgaggcgag
    18121 tagctcagcc agatgctgac gtgaggcaac atccaggcca tcaaacggtt catcaaggat
    18181 caacaagtca ggctccgaca tcagcgcctg acacagcagg gtttttcgcg tctcgccagt
    18241 ggaaaggtat ttaaagcgtc ggtcgaggag ggcggtaata ccgaactgct gcgccagttg
    18301 catgcaacgc gaagtatcct ttacttcatc ctgaatgatc tcagccgtgg tacgcccggt
    18361 gtcatcttcg ccaggactaa gcatatcggt gttattccgc tgccattcgt cgctgacgag
    18421 cttttgcaat tgctcgaagg agagacgagt gatgtgggaa aactggcttt gccgttcacc
    18481 tttcaaaagc ggaagttccc ccgacagcgc gcgggccagc gccgatttcc cgcttccatt
    18541 cgaaccgaca aacgcccaac tatcacccgc gtttaacgtt agccgaggca attgcagcgt
    18601 ttttgtgtcg ccaagacgaa acgtgccttg caaaatttgc aacgatgaca ttttatatcc
    18661 cattttgtgc agcgattact gacagggata cgtgtttcat aacaaattgt caacacgctt
    18721 agcacagcgt ggcgataatc acgctgtcgg cattaaagta ggccgtgaca ttctgccctt
    18781 gctgaagaga agtcgcttca tttaccggca ctgtggcgca cagtgtttgc ccgtcgggta
    18841 gcgccattaa tacttcgcac tgctctgcgc cgcgctcaat atgactaata atacccggta
    18901 attggttgtc agcgttttgc gcgaccgcct cgtcctgagt aatacctacc cacggcgctt
    18961 ttagcaatat caacacttct ttgccttcat ccagccccag acgcgcgccg ctttgtgcgg
    19021 taattgcgac tttcaggcgt gtttttccat cagccagtaa gacatcaaca tgctgttgaa
    19081 cgtcatcatg atcgcgagcg gtgatggtac cgaaccactg gttacgggcg ctggtttgca
    19141 gtgaaaaacg tgagatcgcg gccagcaggc tgttcagcgg cagggcgtca tcgtcactta
    19201 acacatcaaa ggctttttgc tggatttgcg ccagtaagtc atagagttga atcagtcgct
    19261 gaccatagcg ggtcagtact gcgccgcctc cacctttacc gcctgttgcg cgctcaacca
    19321 gaatatgctc acttaactga ttcatctcgt taatggcatc ccaggcgctt ttatagctga
    19381 taccggcatc tttcgctccc tggctaatgg aaccggaaag cgcaatgtgt tttagtagcg
    19441 aaatgcggcg cgggtcggcg aataattttt gttggagctt aagggtgaga aggatttcgg
    19501 cctgcataac aatgtcctgg caaaagtctt attgtgacgg aaaacgaacg ccacgcaaag
    19561 ctgaccgcac aaaaggggag tgcttttctg tgcttagcgg ttagaatagt ctcatgacta
    19621 tatctggagt tgaccatgtt agagttatta aaaagtctgg tattcgccgt aatcatggta
    19681 cctgtcgtga tggccatcat cctgggtctg atttacggtc ttggtgaagt attcaacatc
    19741 ttttctggtg ttggtaaaaa agaccagccc ggacaaaatc attgattccc tgaatgcccg
    19801 cttagtcggg cattttcttt ttctcaactt cctgcttttt ctgccgatat tttttctcat
    19861 ctacctcaca aaggttagca ataactgctg ggaaaattcc gaacttgccg ttatattgac
    19921 gcctacataa cgttagatta aggggttacc aatggctcgt aaatggttga acttgtttac
    19981 cggggcggca ctctctttcg ctgttgctgg caatgcactg gcagatgaag ggaaaatcac
    20041 ggtgttcgcc gccgcatcac tgactaacgc aatgcaggac attgctacgc agtataaaaa
    20101 agagaaaggc gtggatgtgg tttcttcttt cgcttcgtca tctactttgg cccgtcagat
    20161 tgaagcgggt gcgcctgcgg atctgtttat ttctgccgat cagaaatgga tggattatgc
    20221 ggttgataaa aaagcgatcg atacggcttc gcgccagaca ctgctcggca atagcctggt
    20281 cgttgtagca ccgaaagcca gcgagcagaa agatttcacc atcgacagca aaaccaactg
    20341 gacttcgctg ctgaatggcg gtcgcctcgc ggttggtgat ccggaacatg tccctgctgg
    20401 catttatgcg aaagaagcac tgcaaaaact gggcgcatgg gatacgcttt ccccgaaact
    20461 ggctccggcg gaagatgttc gtggcgcgct ggcgttggtc gaacgcaatg aagcgccgct
    20521 gggtattgtt tacggttctg acgcagttgc cagcaaaggg gtaaaagtgg ttgccacctt
    20581 cccggaagat tcacataaaa aagtggaata tccggttgct gttgtggaag ggcataacaa
    20641 tgcgacagtg aaagcatttt atgattatct gaaggggccg caggctgcgg aaatctttaa
    20701 acgttacgga tttacaacca agtaatgata ctgaccgatc cagaatggca ggcagtttta
    20761 ttaagcctga aagtttcttc cctggctgtg ctgtttagcc tgccgtttgg gatctttttt
    20821 gcctggttac tggtacgttg cacgtttccg ggcaaagctc tgctcgacag cgtactgcat
    20881 ctaccgctgg tgttaccgcc cgtggtcgtc ggttacctat tattagtttc gatgggacgg
    20941 cgcggattta tcggtgaacg tctgtatgac tggtttggtc tttctttcgc gtttagctgg
    21001 cgtggtgcgg ttctcgctgc cgctgtcatg tcttttccgc tgatggtgcg ggcgattcgt
    21061 ctggcgctgg aaggtgttga tatcaaactg gagcaggccg caagaacact gggggccggg
    21121 cgctggcgcg ttttctttac tatcacgtta ccgctgacct taccgggaat tattgttggt
    21181 acggtactgg cattcgctcg ttcgctcggt gaatttggcg caaccatcac ctttgtgtcg
    21241 aacattcctg gtgaaacgcg gaccattcct tctgccatgt ataccctgat ccagacccct
    21301 ggcggggaaa gtggcgcggc gagactgtgt attatttcta ttgcgctggc gatgatctcc
    21361 ctgttgattt cagaatggct ggccagaatc agccgtgagc gggcggggcg ctaatcatgc
    21421 tggaactgaa tttttcccag acgttgggca accattgcct gacgattaat gaaacgctgc
    21481 ccgccaatgg catcaccgct atttttggtg tctctggtgc cggaaaaacc tcgctgatta
    21541 acgccatcag tgggctgaca cgtccgcaaa aagggcggat tgtcctcaat ggtcgggtac
    21601 taaatgacgc cgataaaggt atctgcctga cgccagaaaa gcgtcgcgta ggctatgtgt
    21661 ttcaggatgc gcggctgttt ccgcattata aagtgcgtgg caatttgcgc tacggcatgg
    21721 cgaaaagcat ggtcaatcag ttcgataagc tggtggcgct tttaggcatt gaaccgttgc
    21781 ttgatcgttt accaggcagc ctgtccggag gcgaaaaaca gcgcgtggcg attggtcggg
    21841 ctttgctgac agcaccggaa ttgctgttgc tggatgaacc gctggcatca ctggatattc
    21901 cgcgtaaacg cgaactgttg ccttatctgc aacgtctgac gcgggaaatc aacattccga
    21961 tgttgtatgt cagccattcg ctggatgaga tcctccatct ggcagacaga gtgatggtac
    22021 tggaaaacgg tcaggtgaaa gcctttggcg cgctggaaga agtctggggt agtagcgtga
    22081 tgaatccgtg gctaccgaaa gagcaacaaa gtagcattct gaaagtgaca gtacttgagc
    22141 atcatccgca ttacgcgatg accgcgctgg cgctgggcga tcagcatttg tgggtcaata
    22201 agctggacga accgctgcaa gctgcgctac gcatccgcat tcaggcttcc gatgtctcac
    22261 tggttttaca accgccgcag caaaccagca ttcgtaacgt attgcgggca aaagttgtta
    22321 atagttatga cgacaacggc caggtggaag tggagctgga agtcggctgt aaaacgctgt
    22381 gggcgcgtat cagcccgtgg gccagggatg aactggcgat caaacctggc ctgtggctgt
    22441 atgcgcaaat taaaagtgtg tcgataaccg cctgattaaa tcaggtggct ataaatgaac
    22501 tgggcaatgc tgtcggtggt gttatcacca atcacaatgt tggcgcgcgc ttttactgcg
    22561 tcatcggcgt tgcccatcgc cacgcctgta ccagcggctt ccagcatact aatatcattg
    22621 aagttatcgc cgaacgccac gacgttttcc atcgaccagc cttgcgcctc aacccatttc
    22681 gtcaaacgtt tacctttgct gttgccgccg cgtgcaatat caacctgatc gtgccaggac
    22741 cattcacact ccagtcccag ttcatgttcg acatgcttac caaaatgctg caattgcggc
    22801 aggtcatcgt gcgtcagggc gaacttccat acggcgttaa cttgttgcgc cgtttcagcc
    22861 agagaagcga cttgtgtgaa agtcggacgc tgttccggcg gcagggtttg cgcccagtta
    22921 gacgtgcgaa tgacatgtcc ggtcgggtgc tcatagacca tcgcatcatc cacatacatc
    22981 agaccgtgaa tgtggtgttc attcagcatc tcaatgagtt gcagggcttt attaacgggc
    23041 attgggtccg cttccagcac ggtttttgca tgataatcat acaaataggt gccattacag
    23101 caaatagcag gtgtatccag cgccagcgcc tgataaaaag gatgaatagc gacgtgatgg
    23161 cgacctgtga cgatgattaa tcgatagcct gcttcgcgag cgcgggccag ggcttctatc
    23221 gatgatggaa gcagggtctt tttcggggtc aataaggtgc cgtctaagtc gagagcaatc
    23281 acgcgtgtgg tcatggcgta ttccagatta aggttaagaa ttttcgtctg cgcgaatggt
    23341 acaccgatac cactatcgga caaaattctg cgttttaatt cagcattcac cgccaaaagc
    23401 gactaatttt agctgttaca gtcagttgct aaatgcaaag gagcattcat gaagcaaaca
    23461 gtttatatcg ccagccctga gagccagcaa attcacgtct ggaacctgaa tcatgaaggc
    23521 gcactgacgc tgacacaggt tgtcgatgtg ccggggcagg tgcagccgat ggtggtcagc
    23581 ccggacaaac gttacctcta cgttggtgtc cgtccggagt ttcgtgttct ggcgtatcgt
    23641 attgcaccgg atgatggcgc gctgaccttt gctgcagagt ctgcgctgcc gggtagcccg
    23701 acgcatattt ccaccgatca ccaggggcag tttgtctttg tcggttctta caatgcgggt
    23761 aacgtgagcg taacgcgtct ggaagatggc ctgccagtgg gcgtcgtcga tgtggtcgag
    23821 gggctggacg gttgccattc cgccaatatc tcaccggaca accgtacgtt gtgggttccg
    23881 gcattaaagc aggatcgcat ttgcctgttt acggtcagcg atgatggtca tctggtggcg
    23941 caggaccctg cggaagtgac taccgttgaa ggggccggcc cgcgtcatat ggtattccat
    24001 ccaaacgaac aatatgcgta ttgcgtcaat gagttaaaca gctcagtgga tgtctgggaa
    24061 ctgaaagatc cgcacggcaa tatcgaatgt gtccagacgc tggatatgat gccggaaaac
    24121 ttctccgaca cccgttgggc ggctgatatt catatcaccc cggatggtcg tcatttatac
    24181 gcctgcgacc gtaccgccag tctgattacc gttttcagcg tttcggaaga tggcagcgtg
    24241 ttgagtaaag aaggcttcca gccaacggaa acccagccgc gcggcttcaa tgttgatcac
    24301 agcggcaagt atctgattgc cgccgggcaa aaatctcacc acatctcggt atacgaaatt
    24361 gttggcgagc aggggctact gcatgaaaaa ggccgctatg cggttgggca ggggccaatg
    24421 tgggtagtgg tcaacgcgca ctaaccgctg atttaaccgg cgcagtctct cctgcgccgg
    24481 tgtattaacc tatctcctgt aacgcatgtc tctggcgttc gacgatattg gtccacaaat
    24541 tgtctttatc gtcagtccac aaattaatca gcaaggcaaa aaagcgttca gctgccggag
    24601 aaagcacggc atctttgcgg cgaataatcc ccaatgtccg acgtataacg ggttccacca
    24661 gtgggatacc aataatcgaa gaatagggcg catggggcat tgccagacca ggaagcgccg
    24721 aaatccccag tcccgcctcc accagcccta atgacgtcga aagatgacgc acttcgtaaa
    24781 accagtccag cttccagggc ttgtcggcta gctgttgttc tatcaacagt cggttgccgc
    24841 tggaggagcg tacgccaatc attttgtaac ccactaactc ctgccattct acaagctgct
    24901 ttttggccaa tggatggtca cgccgacagg ccagcacgaa cggctcgttg accagtgggg
    24961 taaaatcaat ggatgaattt gtgacgttgt tcatgtttat gccaaagtct gattcgttac
    25021 acagcaccga ttccatgcaa ttattcgtgc cttgttccag aatccgcact ttaatattgg
    25081 gatacagctc attaaattta ccgattgcca atggtaaaaa ataaaatacc gcggtcggaa
    25141 tacacgctaa cgtcaccata cctcgatgat aagcattcat gtcgcgaata ctaaaaagcg
    25201 tttcatcgaa tttttttatt aattcccgcg cctccgggag caaccttttt cctgcttttg
    25261 ttaacgtaac cttgcgagtt gtacgctcaa atagttgaat gtgtaaatct tcctccatct
    25321 tttttattct tcgcgttaat gcaggttgcg taatattgag taatttagca gcattgttaa
    25381 atgaactgga ctctgccagt atgacaaatg ccttcatact tgataattca tgcttcatta
    25441 ttactccgga aaatggaagc gacgattttg ggtgactggc agttaa
//
LOCUS       DAPQZD010000059        25385 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPQZD010000059 DAPQZD010000000
VERSION     DAPQZD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.059"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(39..1898)
                     /gene="gss"
                     /locus_tag="RO454_002375"
     CDS             complement(39..1898)
                     /gene="gss"
                     /locus_tag="RO454_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDY0934195.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2103..2969
                     /gene="yghU"
                     /locus_tag="RO454_002376"
     CDS             2103..2969
                     /gene="yghU"
                     /locus_tag="RO454_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDY0934196.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3039..3287)
                     /gene="hybG"
                     /locus_tag="RO454_002377"
     CDS             complement(3039..3287)
                     /gene="hybG"
                     /locus_tag="RO454_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDY0934197.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3300..3641)
                     /gene="hypA"
                     /locus_tag="RO454_002378"
     CDS             complement(3300..3641)
                     /gene="hypA"
                     /locus_tag="RO454_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDY0934198.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3634..4122)
                     /gene="hybE"
                     /locus_tag="RO454_002379"
     CDS             complement(3634..4122)
                     /gene="hybE"
                     /locus_tag="RO454_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDY0934199.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4115..4609)
                     /locus_tag="RO454_002380"
     CDS             complement(4115..4609)
                     /locus_tag="RO454_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDY0934200.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4609..6312)
                     /gene="hybC"
                     /locus_tag="RO454_002381"
     CDS             complement(4609..6312)
                     /gene="hybC"
                     /locus_tag="RO454_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDY0934201.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6309..7487)
                     /gene="hybB"
                     /locus_tag="RO454_002382"
     CDS             complement(6309..7487)
                     /gene="hybB"
                     /locus_tag="RO454_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDY0934202.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7477..8463)
                     /gene="hybA"
                     /locus_tag="RO454_002383"
     CDS             complement(7477..8463)
                     /gene="hybA"
                     /locus_tag="RO454_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDY0934203.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8466..9584)
                     /gene="hybO"
                     /locus_tag="RO454_002384"
     CDS             complement(8466..9584)
                     /gene="hybO"
                     /locus_tag="RO454_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDY0934204.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9773..10060)
                     /locus_tag="RO454_002385"
     CDS             complement(9773..10060)
                     /locus_tag="RO454_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDY0934205.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10179..11066)
                     /locus_tag="RO454_002386"
     CDS             complement(10179..11066)
                     /locus_tag="RO454_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDY0934206.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKSNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11223..12263
                     /gene="gpr"
                     /locus_tag="RO454_002387"
     CDS             11223..12263
                     /gene="gpr"
                     /locus_tag="RO454_002387"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDY0934207.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12303..12797)
                     /locus_tag="RO454_002388"
     CDS             complement(12303..12797)
                     /locus_tag="RO454_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDY0934208.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            12988..13872
                     /gene="yghA"
                     /locus_tag="RO454_002389"
     CDS             12988..13872
                     /gene="yghA"
                     /locus_tag="RO454_002389"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDY0934209.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13911..14336)
                     /gene="exbD"
                     /locus_tag="RO454_002390"
     CDS             complement(13911..14336)
                     /gene="exbD"
                     /locus_tag="RO454_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDY0934210.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14343..15077)
                     /gene="exbB"
                     /locus_tag="RO454_002391"
     CDS             complement(14343..15077)
                     /gene="exbB"
                     /locus_tag="RO454_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDY0934211.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15329..16516
                     /gene="metC"
                     /locus_tag="RO454_002392"
     CDS             15329..16516
                     /gene="metC"
                     /locus_tag="RO454_002392"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDY0934212.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16656..17315
                     /locus_tag="RO454_002393"
     CDS             16656..17315
                     /locus_tag="RO454_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0934213.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17355..18254)
                     /gene="yqhC"
                     /locus_tag="RO454_002394"
     CDS             complement(17355..18254)
                     /gene="yqhC"
                     /locus_tag="RO454_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDY0934214.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18448..19611
                     /gene="yqhD"
                     /locus_tag="RO454_002395"
     CDS             18448..19611
                     /gene="yqhD"
                     /locus_tag="RO454_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDY0934215.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19716..20543
                     /gene="dkgA"
                     /locus_tag="RO454_002396"
     CDS             19716..20543
                     /gene="dkgA"
                     /locus_tag="RO454_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDY0934216.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20743..21669
                     /locus_tag="RO454_002397"
     CDS             20743..21669
                     /locus_tag="RO454_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDY0934217.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALILNDNLTLPGNIGWLDYDPVCDCQDFGNLVLESVAITQTD
                     ADHADAAVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     TIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21720..21977
                     /gene="yqhH"
                     /locus_tag="RO454_002398"
     CDS             21720..21977
                     /gene="yqhH"
                     /locus_tag="RO454_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDY0934218.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22019..24238)
                     /locus_tag="RO454_002399"
     CDS             complement(22019..24238)
                     /locus_tag="RO454_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDY0934219.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKS
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICSHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24490..25239
                     /locus_tag="RO454_002400"
     CDS             24490..25239
                     /locus_tag="RO454_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDY0934220.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIISLQENAIAAESDRFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
BASE COUNT         6330 a         6739 c         6351 g         5965 t
ORIGIN      
        1 tattcttcat aatcagtacg gggttgctac aacatgtatt actttttcac cacaattaac
       61 ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa acacgtccca
      121 ccgtagttgc cgccaacggt gaaggtacat acctgaatgt atttaccgtc cactttcggc
      181 aaacaccaca gttgctggta aatatttttc tgttcggcaa atttaccact ggtctgatca
      241 agcacctctt catgatggct gaccaggtca atgttgctac cacagcgccc ggcaatcggt
      301 ttaacggcat aaccggtttt caccagctca tcattcacgg tgaagtcagt atccaacaga
      361 taacgatggt gcgggaacag agaccagagg atcggcagaa tcgctttgtt accggggatc
      421 accgtccata gcggctcaaa aaccaacact tccgggcgca gcaatacgtc gataagacgc
      481 acttcatttt gtggatgccc ggtacggatc ggcactgcgg caaattcacg gtcgctcact
      541 tcgcggattt gatcaaatgc cgtttcccac gcccaggttt tccatacgca gttaaccagt
      601 cgcccttccc catcaatcag ttgcccggca gcatcccagc ccagttcatc caacccacgc
      661 aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg cgcgtgatag
      721 ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcacg actgtgtttc
      781 cacgcgccag ccagttcgtt aatcagtcct tccgcaggat tgaagccgtt gcctttatag
      841 ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacagga ggcagaatcg
      901 gcgttgtatt cgtagacttt caggccacgt tcatccatgc agaaatccat acgaccagtg
      961 atcatatgat gacggcgacg ctgccaggag agacgcaaac gtggccagag gattttcgga
     1021 atgtcaaaca gcgccagcag gttgtcatct ttcagcacct tgtcggtggc gtgaagatac
     1081 atcaggtgca gctcgttggt ggctttaatc agctcctgct cggcactctc ggtaatggtg
     1141 tagtaatgat acggatcctg attgatcacc tggccgttgg cctgtacata ggcgttttgc
     1201 agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc cagacgcgcc
     1261 ccgctgattt tcagcagttc gcctgcaatt tccggctgtg gcaagctgta ttcggtatct
     1321 tccgtctgga tcatccagcc cagaatagtg gtgtcatcga aggtgtcttt cagggtatag
     1381 ccgccgtttt cgaccaccat ctccagctcg cgtgtccact gttgcccttg cggcaacggg
     1441 gtatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg ggtaatgatg
     1501 gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag cgcccccgct
     1561 accggtgcac gcggcgaacc gttaggaaat gcctgcaatg gcaggatgtt gtcattgacc
     1621 acttcacgca ggaagcgcag tgagaaaatc tcccatgcca tacccacgtc agtgaagact
     1681 acaccgtagt tcagaaagag aaaacggcga gcaaattcaa cgcattgcca tttgtggccc
     1741 atatattcat cgtcgatata gctacggaat acggcgtcat cttcgtattc ctgcggatcg
     1801 agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc caataatgtc
     1861 ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt aacaatacaa
     1921 cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat taaccggaca
     1981 gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta aaaaattcac
     2041 attctgtaca gcaagtgacc tgctacactg cttcaacact accactcaga aggcaactca
     2101 ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa tccgctggcg
     2161 gcgcgttcgc cagtatcaat cgcccggttt ctggtccgac gcatgaaaaa acgctgccgg
     2221 tgggcaaaca tccattgcaa ctttattcgc tgggaacgcc gaacggtcag aaagtaacga
     2281 ttatgcttga ggagctgctg gcactgggcg ttactggtgc agagtacgac gcctggctga
     2341 ttcgtattgg cgatggcgat caattctcca gcggctttgt cgaagtgaac ccaaactcga
     2401 agatcccggc gctgcgcgac catacccata atccaccgat ccgcgtgttt gagtctggat
     2461 cgattctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat ttggcaaagc
     2521 gtactgaaac gatgaactgg ctgttctggt tacagggcgc ggcaccgttc ctcggcggtg
     2581 gttttggtca cttttaccat tacgcgccgg tgaaaattga gtacgccatc aaccgcttta
     2641 ccatggaagc caagcgcctg ctcgacgtgc tggataagca actggcgcag cacaagtttg
     2701 ttgcgggcga tgagtacacc attgcggata tggcgatctg gccgtggttt ggcaacgtgg
     2761 tgttaggcgg cgtgtatgat gccgcagagt ttctggatgc gggcagttat aagcacgtgc
     2821 aacgctgggc gaaagaagtg ggcgaacgtc cggcggtgaa gcgtgggcgt attgttaacc
     2881 gcaccaacgg gccgctgaac gagcagttgc atgagcgcca tgacgccagt gatttcgaga
     2941 cgaatacgga agataagcgt caggggtaag tcgcaacatt ggcaggctta tcgctatgta
     3001 gccggataaa gcatttacgc cgcatccggc tttaatcatc acgcgctggt aatgtcgtaa
     3061 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3121 gcaaatccga cgtgtaccag cacccactgg cccagcagat cggcagggtt accttcacaa
     3181 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3241 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt taactccagt
     3301 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
     3361 ccatgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3421 cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
     3481 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
     3541 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg
     3601 ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgccac tcctgcgacc
     3661 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttagcgaccg gtagcgaaag
     3721 gatcatccgc gcgcagtcat ccgtcaggcg ttggccctct tcaatcgaca tgctatgcga
     3781 aagcggtgac atcagcgaac aggagagata ttgagaaaca ctgtccagtt cgccaacggt
     3841 aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg
     3901 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
     3961 ccccgtccac tgaccttcga acagcgtaaa gtcagaaaca tacaccggca ttgagggatg
     4021 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
     4081 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgagtcgcc tcccgtggga
     4141 tagcttccac gcctgattcg cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca
     4201 ttgcttcaac cgtcggcgtt aagccgatgt gcggctccag cgattccggg atcacaccga
     4261 ccagcgtcag ctttttcgga aactcgccgg tgaagcgcag ggccgacaat acgtcggcca
     4321 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
     4381 ggatcatcat cgttcccggc gcgttctttt tcgacacaat ggcatccgca ataatcagat
     4441 gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct
     4501 caacataatc cggcagaatg tatcgttgct ctaacgcttc aacaatccgc acaccgatgg
     4561 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
     4621 cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg
     4681 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcgg caaccggtgt
     4741 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
     4801 ccaggttgat ggaacaaccg cctggtagtt gctgatgata ccgtctttaa taaccatcca
     4861 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
     4921 cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
     4981 actgtattgg ttttgcagga tatcctgcaa ttcacagcag tgaacggtac gaccaataat
     5041 acggcccagc gtggagtgca gctgtgccac ttccagcgtg ttgccagtca gtttctgata
     5101 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
     5161 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gcgatttcac
     5221 ccaggaatat ttaccgtcat cagaccaacc gtcgtaagcc ggaatggtgg taccttccca
     5281 cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
     5341 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
     5401 aatgtagccg cccgggaaca ggaagctgcc gtttttgctg tcggtcggga attccggcac
     5461 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcgat
     5521 aactgcggta tcaaccttat aaacctgttc aacaaagtcg ctcagcttgt cgatgaaaga
     5581 cttgatgtac atcaggcgct caaggttcag cacgcccaaa ccgtcgaggt tgattgggtt
     5641 agcgaccccg cctaccgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
     5701 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag
     5761 gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag tagccattgg cgaaaatacc
     5821 caactgaccg ctggcaacca gatctttaat cttgttctga actttggtga actcttccgg
     5881 actgttcaga tgccaggtcg aaacgccttt cagcatttca gaggctttgg ttgggtcagc
     5941 ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat
     6001 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
     6061 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
     6121 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
     6181 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
     6241 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
     6301 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
     6361 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
     6421 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
     6481 gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac
     6541 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag
     6601 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
     6661 gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat cgccagcaac
     6721 acactgatgg tgttggtcag cttgacgaac aggctctttt catccggacc gttgccacgc
     6781 agacccgcct gcaccagtga accttcaaag atgacaatcg agaagcccat gatgaacgcc
     6841 gtcagcagcg agaacagcgg caacatttca tagctctgcc acagcggatg caccttgtag
     6901 cccgccgaga tcatcagcga ccccattgaa gactggtgca tggtcggcag cagcgcaccg
     6961 agcgcgatga tgaagaacat caccttgttc agacgctgta gcgacacctt ccagcccaga
     7021 cgttcaaaca gtgccggagc aaactccagt gccatcacgc cgatatagat ggtcatacag
     7081 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
     7141 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacctagtga gtaaccaaac
     7201 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
     7261 accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccgatcaa caggtcaaac
     7321 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
     7381 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
     7441 ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat cgtctcctcc
     7501 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
     7561 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
     7621 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
     7681 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
     7741 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
     7801 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac caaaaatcac
     7861 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
     7921 acctttctgg ttgcacaact cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
     7981 cttcggcacg ttgtatgggc aggcgaccat gcagtaacgg cagccggtgc acacatcttt
     8041 gtcgtaatgg acaatgccgg ttttcggatc ttttttcagc gcagagaccg ggcacacaga
     8101 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccattctc
     8161 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
     8221 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggga
     8281 gttgatatcc tgacacttgg tgacgcaagc ctgacagcct acgcacaagg tcgagtcgta
     8341 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
     8401 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
     8461 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
     8521 accgccatca cgctgacacc ggcaaccagc ccaaccactc cgccgagcaa accaatagcg
     8581 cctgccgaga cgttgccgcc ttctttagcg ttaacgtccg gtttctgtga acgcggagtc
     8641 tggttttcga cgttggcaag ctgatggatg cctttatgga agccgatacc ttcttcgtta
     8701 cagccatagc acgggtgacc aatcgctacc ggccacacgc cgccaacatc gcagaattgc
     8761 agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag
     8821 ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
     8881 cgacgttcac agtgttcgtg aatcagacga ccataggcga aagtcggacg gtttttgtca
     8941 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgccag gaagttgtgc
     9001 gggttcggcg ggcagccagg aatattgata acggttttgc ccggtagaac ttcttgcagg
     9061 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc agagcaggaa
     9121 ccgatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc
     9181 tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccaccact
     9241 aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc gacctgatga
     9301 ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag cacgaggttt
     9361 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
     9421 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcgaccat ctctgcagcg
     9481 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
     9541 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
     9601 gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
     9661 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc actaaaacga
     9721 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
     9781 ctaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
     9841 aatccattac catctcttta tccagtccat aacggcgcgt cagaataccc gcttcccagg
     9901 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
     9961 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    10021 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgctata
    10081 tattgtcata tatagcgata ttttaggcaa agaacgacat ttgcgcacgc tattttttca
    10141 tgcttgttta agacgtaatc ctgtaagaga taaacccatc aggagagata tttatcgaac
    10201 catttcagcg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    10261 tcattatgga atccgtgatt aacccccgga tatatatacg cctcatagac cttattattg
    10321 gctttcaacg ccgcctcgta agcgggccag ccctcgttga ttcggctatc cagttcagcg
    10381 tagtggagca gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgccca
    10441 taaaacggca ccgcgcaggc cagttccgga tacgcgactg ctgccgcgtt cgatacgccc
    10501 ccgccatagc aaaatccgct aatacccact ttgcctgtcg cttgcggata gcgttgcata
    10561 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagt
    10621 tcgcggcctt tatcatcgtt accaggatag ccacctactg agcttaaacc gtctggcgcc
    10681 agggcgatgt acccagcctt cgccactcgc cgcgtcacat cttcgatata cggattcagc
    10741 cctcgattct catgcaccac taccacggct ggcgttttgc cgctcatttt tgcgggcttc
    10801 accagataac cccgtacctc gccgtgacca tttggcgagg gatacgtgat gtactcagca
    10861 acaatttccg ggtcggtaaa ctctacctga gtcgccaacg cataatttga cttgagcaaa
    10921 tcaaacagcg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
    10981 tcacgtttcg agattttccc gtgagcgtaa tagtcgtagt aatccagcaa ctcttgtggg
    11041 aaatctttgg cggttaaacg cggcatcgtt gcactcctca actggtgttt ttttaagcaa
    11101 agcataagcg cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac
    11161 gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacgatgg aaaggagagg
    11221 tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcagtaccgc tattgcggaa
    11281 aaagtggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta
    11341 acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttaggc attacgcact
    11401 ttgatttagc caacaactac gggccgcctc caggaagcgc agaagagaac tttggtcgcc
    11461 tgctgcgcga ggattttgcc gcttatcgcg atgaactgat tatctctacc aaagccggat
    11521 acgatatgtg gcccggccca tacggctctg gcggctcacg taaatacctg ctcgccagcc
    11581 tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac tctcatcgcg
    11641 tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgct gtacaaagcg
    11701 gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg
    11761 agttgctgcg cgagtggaaa attccgctgt taattcatca accttcgtac aatttactga
    11821 accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgca
    11881 ttgcctttac tccactggca cagggattgc tgaccggaaa atatctcaac ggtattccgc
    11941 aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg aaaatgctca
    12001 ccgaagccaa cctcaacagc ctgcggttat tgaatgaaat ggcacagcag cgtggacaat
    12061 caatggcgca aatggcgtta agctggttgc tgaaagatga tcgcgtgacg tcggtattga
    12121 ttggtgccag ccgcgcggag caacttgagg agaacgtgca ggcgctgaat aatctgacat
    12181 ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggcgag ctgaatctgt
    12241 ggcaggcgtc ttccgataaa tgacctgtta ataacgggcg cgagaaccgc gcccgaatca
    12301 gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc agagagcaca
    12361 aacgtcagat ggataatgac gtaccacatc agtttgttat ccgggacatt tttcgcatcc
    12421 ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt
    12481 ttcagcgacg ttgcatccat tttccccagc cagttcagct tctctttgtt ctcggagata
    12541 tccagttgtg agacgaaatt ctcgtaaccg gaaaacatca ccatcaccag caaaccgcca
    12601 accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag
    12661 aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac
    12721 gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt
    12781 tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgagcaacag tatatcgcaa
    12841 ttaagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt cgcataattc
    12901 tgagggttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag
    12961 gacgttgaac ctgaagggag aaaaatgatg tctcatttaa aagacccgac cacgcagtat
    13021 tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc gaagatgaca
    13081 ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa
    13141 gcactggtga cagggggcga ttccggaatc ggtcgcgctg ccgccattgc ttacgcgcgt
    13201 gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc tcaggatgtg
    13261 aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga tttaagcgat
    13321 gagaaattcg cccgttcgct ggttcacgaa gcacacaagg cgttaggcgg gctggatatt
    13381 atggcgctgg tagccgggaa acaggttgcc attcctgata ttgcagacct caccagcgaa
    13441 cagtttcaaa agacctttgc cattaacgtt ttcgcgttgt tctggctaac ccaggaagcg
    13501 atccccctgc taccgaaagg tgcaagtatc atcaccactt cctcaatcca ggcataccag
    13561 ccaagcccgc atttactgga ctatgcggct acgaaggcgg caattctgaa ctatagccgt
    13621 ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc gccaggcccg
    13681 atctggacag cactgcaaat ttctggcgga caaacgcagg ataagatccc gcagtttggt
    13741 cagaaaacgc cgatgaagcg tgcggggcaa ccggcggagc tggcccctgt atatgtttac
    13801 ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggtgtctg cggcggcgag
    13861 catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattt ttatttcgct
    13921 ttcgcagttt cttcgccgac cagacctatt ttcaggtaac ccgcctgatg cagcgtatcc
    13981 attaccttca tcaacgtctc gtaatcgacg gttttatccg ctcggaagaa gatggtggtg
    14041 tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc ggtgaccgga
    14101 tcgttaccaa taaacatcga gttgtctgct ttcaccgaca gataaaccgg tttttccggg
    14161 cgcggctgcg gcgtactggt agaagcaggc aaattcacct tcacatctac cgtcgctaac
    14221 ggtgccgcca ccataaagat aatcagcaaa accaacatca cgtcgataaa cggcgtcacg
    14281 ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat cgccatcgga
    14341 cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat gtgcagcggc gctggcttcc
    14401 agatccaggt cacggctttg cagcaacaat acctgcgctg caacatcacc cagcatcgct
    14461 ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgcagg aatcgctgcc
    14521 acgaggccga ttgccgttgc taacagagct tctgcaatac ccggcgcaac gactgccagg
    14581 ttagtggtct gcgtttgcgc gataccaata aagctgttca tgatgcccca gaccgtacca
    14641 aacagaccaa cgaacggcga aatcgcgccg atggttgcca gatagccgtt accacggccc
    14701 atttgacgac ctactgcggc gacccgacgc tccagacgga agctggtacg ttctttaata
    14761 ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc attgagcaaa
    14821 tgcaggctca agcttttgct accaaaatca gcggcgatat cgttggcctg gtttaaggaa
    14881 cgcgcttcag ccagcaattg ctgctcgcgc ttaagacgac gcttctgatt gaagaactcc
    14941 acgctcttac tgaagaagat tgcccaggtg actacggagg ccaaaataag cccaatcatc
    15001 acgcacttaa cgacaatatc ggcgtgctga tacatacccc agacggagag gtccgtctgc
    15061 attaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg tgcataataa
    15121 tatcaaaacg acgtcgaatt gatagtcgtt ctcattacta tttgcatact gccgtacctt
    15181 tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc agcacgccat ttgcgaaaat
    15241 tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta gtttagacat
    15301 ccagacgtat aaaaacagga atcccgacat ggcggacaaa aagcttgata ctcaactggt
    15361 gaatgcagga cgcagcaaaa aatacacgct cggtgcggta aatagcgtga ttcagcgcgc
    15421 gtcttcgctg gtctttgaca gtgtggaagc caaaaaacac gcgacacgta atcgcgccaa
    15481 tggcgagttg ttctatggac ggcgtggaac gttaacccat ttctccttac aacaagcgat
    15541 gtgtgaactg gaaggtggcg caggctgtgt gctatttccc tgcggggcgg cagcggttgc
    15601 taattccatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca acaccgccta
    15661 tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa cgacatcgtg
    15721 gtttgatccg ctgattggtg ccgatatcgt taagcatctg cagccaaaca ctaaaatcgt
    15781 gtttctggaa tcgccaggtt ccatcaccat ggaagtccac gatgttccgg cgattgttgc
    15841 cgccgtacgc agtgtggtcc cggatgccat cattatgatc gacaacacct gggcagccgg
    15901 tgtgctgttt aaggcgctgg attttggcat cgatgtttct attcaggccg ccaccaaata
    15961 tctggttggg cattcagatg cgatgattgg cactgcggtg tgcaatgccc gttgctggga
    16021 gcagctgcga gagaatgcct atctgatggg ccagatggtc gatgccgata ccgcctatat
    16081 aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgc caacatcatg aaagcagtct
    16141 gaaagtggct gaatggctgg cagaacatcc ccaagtagcg cgagttaacc accctgctct
    16201 gcctggcagt aaaggccacg aattctggaa acgagacttt acaggcagca gcgggctatt
    16261 ttcctttgtg cttaagaaaa aactcaatga tgaagagctg gcgaactatc tggataactt
    16321 cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc tggcaaatca
    16381 accagaacat atcgccgcca ttcgcccaca aggcaagatc gattttagcg ggaccttgat
    16441 tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg acgccggttt
    16501 tgcgcgaatt gtataacatt gccacttttg gacaattttg cagacatttt attgtgaaaa
    16561 gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg attcaggagt acaataggca
    16621 gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa gatatcatcg
    16681 ctgcgctctg gcaacacgac tttgccgcgc tggcggaccc tcatattgtt agcgttgttt
    16741 actttgttat gtttgccacg ctgtttttag aaaacggctt gctgcccgcc tcatttttgc
    16801 caggcgacag cttgttgata ctggcaggcg cattgattgc ccagggggtt atggattttc
    16861 tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta agttatattc
    16921 aggggcgctg gttagggaat accaaaacgg tgaaaggctg gctggcacag cttcctgcta
    16981 aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg ctgctggctg
    17041 gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt tccggtctgc
    17101 caaaccgccg cttccagttt ttcaactggt taagcggatt gctgtgggtc agcgtggtaa
    17161 ccagtttcgg ctatgcctta agtatgattc cgttcgttaa acgacatgaa gatcaggtaa
    17221 tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgttg ggcacgctgt
    17281 ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccgttc ccggttgctg
    17341 taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc ttcccccggc
    17401 gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc atagccgacg
    17461 cgcatcgccg ccgcgctggc cttcatgccg tcgtggatga tcatcatccg cgccttatgc
    17521 agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt aaaattatgg
    17581 tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct caggttttcg
    17641 gtgtatttat tctcaatccg tttcagcacg cgactaatca ggctgaagtg agtctggcgg
    17701 ctgaccagtg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta cagaatttcg
    17761 cggataatct gtttgccgag aatgcgcgca tccagcggtc gctccatcac atccagtaac
    17821 cgctctgccg cgcataaaat ctcttctgat aacgtggcgg agttaatacc gctggctgcc
    17881 attgacggct gaaaatgttc atcttcgcca atgtccatca acagttcctg taactgcaaa
    17941 atatcgacat tgagacgcaa ccctgccagc ggcacctctg acgtggcata ggtttcgcac
    18001 tcaaacggca acggcaccgt cagcaggaga tattcattag cgtcataacg aaacacgcgt
    18061 tcattgatat aaccgatttt atgtccggaa aagagaatta tgatgccagg ctcgtacatc
    18121 accggtgtgc gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg caacagctct
    18181 gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa gcggcaaatc
    18241 tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga ttgtgcgcaa
    18301 ttttgtagca tttctccagc actctggaga aataggcaag acattggcag aaatgagcat
    18361 tgagaggtac ggcgctggcg accacaatga aaaacatcag gcagaccgat ctctgccctc
    18421 atattgaccc agcaaaggga gcaagtaatg aacaacttta atctgcacac cccaacccgc
    18481 attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca cgatgctcgc
    18541 gtattgatta cctacggcgg cggcagcgtg aaaaaaaccg gcgttctcga tcaagttctg
    18601 gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgaaccaaa cccggcttat
    18661 gaaacgctga tgaacgccgt gaaactggtt cgcgaacaaa aagtgacttt tctgctggcg
    18721 gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc taactatccg
    18781 gaaaatatcg atccgtggca cattctgcaa acgggcggca aagagattaa aagcgccatc
    18841 ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc aggtgcggtg
    18901 atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcccatgt tcagcccgta
    18961 tttgccgtgc tcgatccggt ttatacctac accctgccgt cgcgtcaggt ggctaacggc
    19021 gtagtggacg cctttgtaca caccgtggaa cagtatgtta ccaaaccggt tgatgccaaa
    19081 attcaggacc gtttcgcaga aggcattttg ctgacgctga tcgaagatgg tccgaaagcc
    19141 ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca tgtgggcggc gactcaggcg
    19201 ctgaacggtt tgatcggcgc tggcgtaccg caggactggg caacgcatat gctgggccac
    19261 gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt cctgcctgca
    19321 ctgtggaatg aaaaacgcga taccaagcgc gctaagctgc tgcaatatgc tgaacgcgtc
    19381 tggaacatca ctgaaggttc agacgatgag cgtattgacg ccgcgattgc cgcaacccgc
    19441 aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct ggacggcagc
    19501 tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact gggcgaaaat
    19561 catgacatta cgctggatgt cagccgccgt atatacgaag ccgcccgcta agctttttac
    19621 gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca cctcaccgga
    19681 gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc gttattaagc
    19741 tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca agtaatgagg
    19801 aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt gataccgccg
    19861 cagcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca gtcaacagag
    19921 aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc cgcgaagccc
    19981 tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctattta atgcactggc
    20041 ccgttcccgc tatcgaccat tatgtcgaag catggaaagg catgatcgaa ttgcaaaaag
    20101 agggattaat caaaagcatc ggcgtgtgca acttccagat ccatcacctg caacgcctga
    20161 ttgatgaaac tggcgtgacg cctgtgataa accagatcga acttcacccg ctgatgcaac
    20221 aacgccagct acatgcctgg aacgcgacac acaaaatcca gaccgaatcc tggagcccat
    20281 tagcgcaagg agggaaaggc gttttcgatc agaaagtcat tcgcgatctg gcagataaat
    20341 acggcaaaac ccctgcgcag attgttatcc gctggcatct ggatagcggc ctggtggtga
    20401 tcccaaaatc ggtcacacct tcacgcattg tcgaaaactt tgatgtctgg gatttccgtc
    20461 tcgacaaaga cgaacttggc gaaattgcaa aactcgatca gggcaagcgt ctcggtcccg
    20521 atcctgacca gttcggcggc taacgtgcaa attatcccgg tggcggtaat gttccgctac
    20581 cggacttttc agaaatcatt tattcccctc gcgtcccgcc cgttgctact cttccttgtt
    20641 caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa aagaacaacc
    20701 catctatatc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat aatacttctg
    20761 tttttagcag ccctggcaag ttttaccgta cacgcacagc ccccctcaca gaccgtagaa
    20821 caaacagtcc ggcatattta tcagaactat aaatcagatg ccactgcccc ttattttggt
    20881 gaaaccggag agcgagcgat aacttctgcg cgtattcaac aggcgcttat cctgaacgac
    20941 aatcttacgc tgccgggcaa tattggctgg ctggattatg atccggtttg tgattgtcag
    21001 gattttggca atctggtgtt agaaagcgta gcgataaccc aaactgacgc cgatcatgcc
    21061 gatgccgctg tgcgctttcg tatctttcaa gacgataaag aaaagacctc gcagacactg
    21121 aaaatggtgg cggaaaatgg tcgttgggtc attgacgata ttgtcagcaa tcatggcagc
    21181 gtcttacaag cggttaatag cgagaatgaa aaaacgctgg ccactatagc ttcgttgcaa
    21241 aaagaacagc cggaagcctt tgttgccgaa ctctttgaac atattgctga ttatagctgg
    21301 ccgtggacgt gggtggtttc cgactcttac cgccaggcgg tcaatgcctt ctataaaacc
    21361 accttcaaga cggccaacaa tcccgatgaa gatatgcaaa tagaacggca atttatttac
    21421 gacaatccga tctgttttgg cgaagagtcg ttattttccc gcgttgatga aattcgagtc
    21481 ctggagaaaa ccaccgattc cgcccgcatt catgttcgct ttacgctgac caatggcaac
    21541 aacgaagagc aagaactgat tttacagcgg cgcgaaggta agtgggaaat cgctgatttt
    21601 atccgcccga acagcggcag cctacttaag cagattgagg caaaaaccgc cgccagatta
    21661 aaacaatgag ctgaattaaa taacaattag ccggaacaat aaataaaagg gaacactata
    21721 tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc agtggctgcg
    21781 tcaatgagca aaaggtcaat caactggcga gcaatgtgca aacattaaat gcaaaaatcg
    21841 cccggcttga gcaggatatg aaagcattac gcccacaaat ctatgctgcc aaatccgaag
    21901 ctaacagagc caatacgcgt cttgatgcac aggactattt cgactgcctg cgctgcttgc
    21961 gtatgtacgc agaatgataa aaaaatcccc ggcagcattc tgttaccggg gatatttttt
    22021 aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attctgcagg cgagattgcg
    22081 tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt
    22141 tagtcaacgc cggacgggta ttgcggttct ggcgacgagc ctcacgcatc tcttcaatag
    22201 ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt
    22261 ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat
    22321 gcaaacgacg ctgtttgtcg cccttcggca cgaagacgtc ttcactctta taaccaatct
    22381 tcgccagcgg gtttttgccg gtgtaataca tggtggtcga gttagccagc ggcgacggat
    22441 agaagttctg cacctggtcg aggcggaagc gatgcttttt cagccatagc gccagattca
    22501 ccatatcttc atcacgcgta cccgggtgcg cggagatgaa atacgggatc agatactgtt
    22561 ctttacctgc ctgtttcgag taagtatcga acagctcttt aaagcggtca tagctgccca
    22621 tgcccggctt catcatcttc gataacggcc cttcttcggt atgttccggg gcaatcttca
    22681 gataaccgcc aacgtgatgg gtcgccagct ctttgatata gcgcggatct tctacagcga
    22741 tgtcataacg cacaccagag gcaatcagga tctttttaat gcctttcaga tcacgcgcac
    22801 ggcgatagag attgatggtc ggttcatgat tagtgtccat gtgcgaacaa atatccggat
    22861 aaacgcacga caaacgacgg caggtttgct cagcgcgtgg cgatttgcag cgcaacatat
    22921 acatgttggc agtaggccca ccgagatcgg aaatcacgcc cgtaaaacct ggaacagtgt
    22981 cgcggatcgc ttcgatctca ttaataatcg aatcttcgga acggctctga ataatgcgcc
    23041 cttcgtgctc ggtaatagaa cagaaagagc agccgccaaa gcagccacgc ataatgttga
    23101 ccgagaaacg aatcatttcg taagccggaa ttcgggcatt accgtaagcc ggatgcggca
    23161 cgcgcttgta cggcagcgca aagacgctat ccatctcttc ggtagaaagc ggaatagcag
    23221 gcgggttgat ccatacatag cggtcgccat gtttttgcat caatgcgcgg gcgcagcctg
    23281 ggttagtttc gtggtgcaga atgcgcgaag catgggcgta cagcacttta tcgcccttca
    23341 ctttctcgaa agaaggcagc aacacgtagg ttttttccca cggtttcggg cgtggtggct
    23401 gcacggttac agatttggct tcctgctttt tcggagctac cggtttgtta tccgcgcacg
    23461 gcaaatcttc gccatacgga tgcgggattg ggtcgatttt cccaggggta tcaagacggg
    23521 tggaatccac accgctccag cctggcaacg cctcttttac gataatcgcg gtattacgca
    23581 catcgcggat ttcactaatc ggctcgccca tcgccagacg gtgcgccact tccaccagcg
    23641 gacgctcacc gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc
    23701 gcacggtatc cgaccagtaa tcatagtgcg cggtacggcg caggctggcc tcaataccgc
    23761 cgaggatcac cggcacatct ttccacgcct ctttacaacg ttgggtataa accagtgtgg
    23821 cgcgatccgg acgcttaccc gcgacattat ctggcgtgta ggcatcgtca tgacgtaaac
    23881 ggcgatcggc ggtatagcgg ttgatcatcg agtccatgtt gccagcagta acaccgaaaa
    23941 acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtct ggctgggcga
    24001 tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc atcccgaagc
    24061 ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa
    24121 gttgatccat ctcttcacgt gacatcggca aaaacggtgc cggtccaaaa caggcggccc
    24181 agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt
    24241 tgcttccaga aatggtaaaa aaataatcaa aggccgggga ttatacgctg tatcacgcgc
    24301 ggaaatgaag tgatgttgat gaacggtggc gaaataccct ggtgaggttt ttcactgccc
    24361 gacttgggca agcacattta aggctggcgg tatcgtggca actgccgcta ctatttatgc
    24421 cgatcagcaa cgttcaaatc atattgcacg acgatcggct atccgaggac acccacagta
    24481 aggcaaggta tggaacaggt tattacaaag cgccgctatt acgatattgg tctacagatt
    24541 gaagagctgc tgtattcagg cgtattcaaa gccggagaac gcttaccttc agaacgtgaa
    24601 ctgagcgagc ggtttaatac cagccgaact accattcgtg aagccatcat tatgctggaa
    24661 ctgaaaggcg tattgaatgt gaagcaaggg tcagggattt tttttgtcga ctccaccgat
    24721 aagctcaacc agaaatcatt aatgccctat tcagaaattg gcccgtttga actgttgcag
    24781 gcgcgtcagg tgattgagag caatattact ggttttgcag ccagccagat tagctttaac
    24841 gagttacaag agttaaagaa aatcattagt ttgcaggaaa acgctatcgc tgcggaaagt
    24901 gatagatttg aagacctgga tcatcgtttt catagcatta ttgccgaagc gacgcaaaat
    24961 cgggtactca tcaaacaagc cgctgaatta tggcgggcag tgcgtacaga aaacccccgc
    25021 tggaagaaac ttaactacaa gtacctgcat gaaaagcatt tgcgtctgca atggctggaa
    25081 gatcaccggg ctattttcct ggctctgcaa cagaaagatt cagagctcgc cagggaagcg
    25141 tcctggagac atctggaaaa cagcaaaaat gaactgatca aaatcttcaa acaagacgct
    25201 tccatcagcg attttgatga ctttttcttc gctcgttaac cttcattagt ccagagctgg
    25261 cgggaaaaat aacgccggct ctgcctttcg ccctccactg gtacacgctc tctttttaat
    25321 tggtctaata aatcgactat catcagatat cttagattag cggcaattat cctaaaaact
    25381 gacct
//
LOCUS       DAPQZD010000060        25132 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPQZD010000060 DAPQZD010000000
VERSION     DAPQZD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.060"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(391..2964)
                     /gene="clpB"
                     /locus_tag="RO454_002401"
     CDS             complement(391..2964)
                     /gene="clpB"
                     /locus_tag="RO454_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDY0934221.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3094..3825)
                     /gene="pgeF"
                     /locus_tag="RO454_002402"
     CDS             complement(3094..3825)
                     /gene="pgeF"
                     /locus_tag="RO454_002402"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDY0934222.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3822..4802)
                     /gene="rluD"
                     /locus_tag="RO454_002403"
     CDS             complement(3822..4802)
                     /gene="rluD"
                     /locus_tag="RO454_002403"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDY0934223.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4937..5674
                     /gene="bamD"
                     /locus_tag="RO454_002404"
     CDS             4937..5674
                     /gene="bamD"
                     /locus_tag="RO454_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDY0934224.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5944..6285
                     /gene="raiA"
                     /locus_tag="RO454_002405"
     CDS             5944..6285
                     /gene="raiA"
                     /locus_tag="RO454_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDY0934225.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6388..6513
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6389..6436
                     /gene="pheL"
                     /locus_tag="RO454_002406"
     CDS             6389..6436
                     /gene="pheL"
                     /locus_tag="RO454_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDY0934226.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6535..7695
                     /gene="pheA"
                     /locus_tag="RO454_002407"
     CDS             6535..7695
                     /gene="pheA"
                     /locus_tag="RO454_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDY0934227.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELVVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7738..8859)
                     /gene="tyrA"
                     /locus_tag="RO454_002408"
     CDS             complement(7738..8859)
                     /gene="tyrA"
                     /locus_tag="RO454_002408"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDY0934228.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLILSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8870..9940)
                     /gene="aroF"
                     /locus_tag="RO454_002409"
     CDS             complement(8870..9940)
                     /gene="aroF"
                     /locus_tag="RO454_002409"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDY0934229.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10150..10515
                     /locus_tag="RO454_002410"
     CDS             10150..10515
                     /locus_tag="RO454_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDY0934230.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            10662..11180
                     /locus_tag="RO454_002411"
     CDS             10662..11180
                     /locus_tag="RO454_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDY0934231.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11170..12396
                     /gene="dgcN"
                     /locus_tag="RO454_002412"
     CDS             11170..12396
                     /gene="dgcN"
                     /locus_tag="RO454_002412"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDY0934232.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12412..12894
                     /locus_tag="RO454_002413"
     CDS             12412..12894
                     /locus_tag="RO454_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDY0934233.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLITQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12971..13318)
                     /gene="rplS"
                     /locus_tag="RO454_002414"
     CDS             complement(12971..13318)
                     /gene="rplS"
                     /locus_tag="RO454_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDY0934234.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13360..14127)
                     /gene="trmD"
                     /locus_tag="RO454_002415"
     CDS             complement(13360..14127)
                     /gene="trmD"
                     /locus_tag="RO454_002415"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDY0934235.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14158..14706)
                     /gene="rimM"
                     /locus_tag="RO454_002416"
     CDS             complement(14158..14706)
                     /gene="rimM"
                     /locus_tag="RO454_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDY0934236.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14725..14973)
                     /gene="rpsP"
                     /locus_tag="RO454_002417"
     CDS             complement(14725..14973)
                     /gene="rpsP"
                     /locus_tag="RO454_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDY0934237.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15110..16471)
                     /gene="ffh"
                     /locus_tag="RO454_002418"
     CDS             complement(15110..16471)
                     /gene="ffh"
                     /locus_tag="RO454_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDY0934238.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16638..17429
                     /locus_tag="RO454_002419"
     CDS             16638..17429
                     /locus_tag="RO454_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDY0934239.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            17450..18736
                     /locus_tag="RO454_002420"
     CDS             17450..18736
                     /locus_tag="RO454_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDY0934240.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18790..19383)
                     /gene="grpE"
                     /locus_tag="RO454_002421"
     CDS             complement(18790..19383)
                     /gene="grpE"
                     /locus_tag="RO454_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDY0934241.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19506..20384
                     /gene="nadK"
                     /locus_tag="RO454_002422"
     CDS             19506..20384
                     /gene="nadK"
                     /locus_tag="RO454_002422"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDY0934242.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20470..22131
                     /gene="recN"
                     /locus_tag="RO454_002423"
     CDS             20470..22131
                     /gene="recN"
                     /locus_tag="RO454_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDY0934243.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22280..22621
                     /gene="bamE"
                     /locus_tag="RO454_002424"
     CDS             22280..22621
                     /gene="bamE"
                     /locus_tag="RO454_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDY0934244.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22683..22973)
                     /locus_tag="RO454_002425"
     CDS             complement(22683..22973)
                     /locus_tag="RO454_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDY0934245.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22963..23400)
                     /gene="ratA"
                     /locus_tag="RO454_002426"
     CDS             complement(22963..23400)
                     /gene="ratA"
                     /locus_tag="RO454_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDY0934246.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23571..24053
                     /gene="smpB"
                     /locus_tag="RO454_002427"
     CDS             23571..24053
                     /gene="smpB"
                     /locus_tag="RO454_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDY0934247.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24268..24630
                     /gene="ssrA"
                     /locus_tag="RO454_002428"
     tmRNA           24268..24630
                     /gene="ssrA"
                     /locus_tag="RO454_002428"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6424 a         6589 c         5863 g         6256 t
ORIGIN      
        1 tgagacttgg tattcatttt tcgtcttgcg acgttaagaa tccgtatctt cgagtgccca
       61 cacagattgt ctgataaatt gttaaagagc agtgccgctt cgctttttct cagcggcgcg
      121 gggtgtgcat aatacgcttt cccgctacag agtcaagcat ttatttttgc tttctctgcc
      181 ggaattctca ggagaacccc gctcacccgg cggcgtgttt gccgttgttc cgtgtcagtg
      241 gtggcgcatt atagggagtt attccggcct gacaagcgaa aaatataaaa actttatcgt
      301 tcgctcattt ttcaggcaaa acatcttaaa tatagtcttt tccgtctaac ttatagacaa
      361 aaacgagccc cgaagggctc gttttatcat ttactggacg gcgacaatcc ggtcttcatt
      421 aacttccagg cgaatcactt tacccggaac caattcacca gacagtattt gctgtgccag
      481 cgggttttca atctgctgct gaattgcacg tttcagagga cgtgcgccat agaccggatc
      541 gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt cataaccacg
      601 ttcttccaga cgtttataca gacgtttcaa ctgaatctgc gcaatcgacg caatgtgctg
      661 ttcacccagc ggatggaaga ccaccacttc atcgatacgg ttaatgaatt ccggacggaa
      721 gttatggcta accacaccga gcaccagctc tttcatgtgc gcataatcca gttcaccgaa
      781 gcgttcctga atcaaatcgg aaccgaggtt agaggtcata atgacaaccg tattacggaa
      841 gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct gcaacagaat
      901 gttgaagaca tccggatgcg ctttttccac ttcatccagc agaatgacgg aatacggacg
      961 acgacgcacc gcttcggtca ggtagccacc ttcttcataa ccgacatatc ccggaggcgc
     1021 accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga tacggaccat
     1081 cgcctcgtcg ctatcaaaca taaagttcgc tagcgcctta caaagctcag ttttccccac
     1141 accggttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg ccagccccgc
     1201 acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac caataacgcg
     1261 atggtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca tcatgcgaga
     1321 aaccggaatc cccgtccaac gcgccagcac ttcagcaatt tcggcatcgg tcactttatt
     1381 acgcaacaga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt gcttttccag
     1441 ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtcaccca cacggcgagc
     1501 ctgttcaata gcaattttcg cctgttccag ttccgcttta atggtctgcg taccagaaag
     1561 cgaggctttc tctgctttcc actcttcttc taactcggag tactgacgtt ctttgtcgct
     1621 cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact ctttcattaa
     1681 cgcctgttgt tccagtttga gctggatgat acggcgatcg agtcggtcga gttcttctgg
     1741 ttttgagtca atctgcatac gaatgctgga tgctgcttca tcgatcaggt cgatggcttt
     1801 atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgctgctg caacaattgc
     1861 cgggtcagta atttgcacat ggtggtgcag ctcgtaacgt tctttcaggc ctcgcagaat
     1921 agcaatggta tcttcaacag aaggctcggc aacaaacact ttctggaaac gacgttccag
     1981 cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtcgtggcac ctacgcagtg
     2041 caattcacca cgcgccaacg ctggtttcag catgtttccg gcgtccattg cgccatcggc
     2101 tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt tgccttcctg
     2161 tttagcaaga tcgttaagta cgccttttaa acgttcttca aactcaccgc gatatttcgc
     2221 cccagccacc agcgcgccca tatccagcgc cagtacgcgg cggcctttca acccttcggg
     2281 cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt taccgacgcc
     2341 gggttcacca atcaataccg ggttattttt agtacgacgt tgcagcacct gaatggtacg
     2401 gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccttgttcgg ctcgttcggt
     2461 aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt gatcgttcac
     2521 gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg tcgcccctgc
     2581 tgctttcagg atgtcggcca gcgtgccgcg agactcaagt gccgccagaa cgaacagttc
     2641 tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat taagaacgcg
     2701 caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgtg gtaaacgatt
     2761 taatgcctga ttgatatctg tgcgcaactg gccagcatta atgccagcgg atgttaataa
     2821 aggactaacc gaaccccctt cctgattcag cagggcgctc attaaatgaa gtggttcgat
     2881 gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa gctggaattt
     2941 attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt gctactggag
     3001 attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg tgaaaagaaa
     3061 atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga aacttgccat
     3121 acgaccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg tatatgtaca
     3181 acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc gggcaagctg
     3241 ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg ctttggcgtc
     3301 tactgccata aacgcctcgc gaacctccgc ccccacttcg aacgcacgtg gcccaattgc
     3361 cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa ccgtctcttc
     3421 caacacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgacct ccgttcccgc
     3481 acgattgcaa aacagcacgg gcaggcagtc ggcagtcatc actgcgcaaa ccgtgccagg
     3541 cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcgccaa taagcttaag
     3601 cacgtctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat tgcccgcagc
     3661 aaaaagtcgc ttgcgattct ccccaacgtg atccgggtta tcgccacaat gggcaccgag
     3721 gttgagcgag tcatacgggg gtaagctcac accgccgata cgggtagagc tacaagccgc
     3781 aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca gtccacttca
     3841 tccttatgct cttcgaaatc ggcgcgcatc tcctcaatca gctccaccat atcttgtgga
     3901 ataggcgcat gccattccat ttcgatgccg gagatcgggt gataaagacg cagcatggtt
     3961 gcatgaagcg cctgacggtc aaacttacgc agcgtggaga taaacgcttc cgaagcacct
     4021 tttggcggac gcggacgtcc accataaacc ggatcgccca ccagcggatg agtgatatgg
     4081 gccatatgca cgcggatctg gtgcgtacgt ccggtttcca gacgcaaccg cagacgcgtg
     4141 tgcacacgga agtgttccat gatgcgatag tgagtgaccg ccggtttacc catgggatgt
     4201 accgccatgt gagtacgttt ggtcgggtgg cggctgattg gttcatctac tgtaccgcca
     4261 gcggtcatat gaccaattgc caccgcttca tactcacgtg taatttcacg ccgttgcaaa
     4321 gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag gccagtggtg
     4381 tctttatcca gacgatggac aatgcccgca cgcggtacat cggcaatggg cggataataa
     4441 tgaagcaatg cattcagtac cgtgccatcc gggttacccg cgccaggatg taccaccagg
     4501 tcgcgcggtt tattaataac gatgatatct tcatcttcat agacgatatc cagcgggata
     4561 tcctgcggtt caaaacgcgc ttcttcttca atctcagcgt tgatggcaac ctgctcgcca
     4621 cccaatactt tttctttcgg cttatcacac actttgccgt taaccagcac acgctggtcg
     4681 aggatccatt cttttatccg cgaacgtgaa taatccggga acatttcggc caaagcctga
     4741 tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac tcgttgtgcc
     4801 atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt aatatagtgt
     4861 gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat tacattttga
     4921 ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccgcca cactaagcct
     4981 gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc caaatgaaat
     5041 ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa taacgcaact
     5101 ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc agctggatct
     5161 catctacgcc tactataaaa acgccgattt gccgttagca caggctgcca tcgatcgttt
     5221 tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc gtggcctgac
     5281 caatatggcg ctcgatgaca gtgcactgca agggttcttt ggcgtcgacc gtagcgatcg
     5341 cgatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc gcggctatcc
     5401 gaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag atcgtctggc
     5461 gaaatatgaa tactccgttg ccgagtatta tacagaacgt ggtgcatggg ttgctgtcgt
     5521 taaccgcgta gaaggcatgt tgcgcgacta tccggatacc caggctacgc gtgatgcgct
     5581 gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg aaaaagtagc
     5641 gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac aaaacggcag
     5701 ccttgagctg ccgttttttt attctgttag ttgtgaaact gaagcgattt agtcactatc
     5761 gatctcatca aatatggccc gctttgagat attcctcaag taaaaaaaca cctcttcctg
     5821 cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga tttccatcac
     5881 acattttgac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca agaggtaaaa
     5941 tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat ccgccaacat
     6001 gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa tccacatatc
     6061 attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac acctaacggc
     6121 gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga attgatcaac
     6181 aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg tgccgcaaca
     6241 tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt tatattgagt
     6301 gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca gtacgggtac
     6361 tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct tcgcattctt
     6421 ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg aagacgaaca
     6481 ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa cactatgaca
     6541 tcggaaaacc cgttactggc gctgcgagaa aaaatcagcg cgctggatga aaaattatta
     6601 gcattactgg cagagcgacg cgaactggtc gtcgaggtgg gaaaagccaa actgctttcg
     6661 catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt aattacgctc
     6721 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     6781 gattccgtat taactcagca ggctttactc cagcaacatc tcaataaaat taatccgcac
     6841 tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag
     6901 tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     6961 tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc
     7021 tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagtttgtc gattgttggc
     7081 gagatgacgt taactatcga tcattgtttg ttggtctccg gcactactga tttatccacc
     7141 atcaatacgg tctacagcca tccgcagccg ttccagcaat gcagcaaatt ccttaatcgt
     7201 tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     7261 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     7321 ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg atttgtggtg
     7381 ttggcacgta aagccattaa cgtttctgac caggttccgg cgaaaacgac gttgttaatg
     7441 gcgaccggac aacaagctgg tgcactggtt gaagcgttgc tggtactgcg caaccacagt
     7501 ctaattatga cccgtctgga atcacgtccg attcacggta atccgtggga agagatgttt
     7561 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     7621 ggggaaatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     7681 gttgatccaa cctgatgaaa aagtgccgga tgatgtgaat catccggcac tggattatta
     7741 ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     7801 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     7861 ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     7921 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     7981 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagta gttgctcaag
     8041 ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     8101 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca accgagcgcc
     8161 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     8221 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     8281 caccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     8341 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     8401 gtggatgggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     8461 ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtatcagca tcttctcgaa
     8521 cagacgtccc atctgaccgc caccaccgac gataaccacc gggcgcagtg acggacaaag
     8581 cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     8641 atcctcaatc agatctggcg gtacacccag agcttccgcc tccgcacgac gcgaggccaa
     8701 catagatgcc tcgcgctccg gaacataaat aggcagtcca aatcggcttt tcacctcgcc
     8761 cacttcagca accaattcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     8821 atcaatttga tcgcgtaatg cggtcaactc agcaaccata ataaacctct taagccacgc
     8881 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     8941 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     9001 cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     9061 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     9121 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     9181 tctctttttc acattgcgca acatccgcag ggctatagtt tggcgcttta ccaccgcgca
     9241 ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     9301 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gccgttgcca
     9361 gactgccatc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     9421 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     9481 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     9541 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     9601 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     9661 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     9721 catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact accagaagac
     9781 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     9841 cttgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     9901 cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc ctgtttatgc
     9961 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
    10021 ttcaatccat atttcgtaca catttcttta cgtaagcaat ttattcgcgc tttttaccct
    10081 tatttctgta tatataaatt tacaccatct ttaaaaacaa tccgctatgc tttgaaaaag
    10141 gagaaagaaa tgatgaaaaa atttatcgcc cccttgttgg ctttactggt tagcggatgt
    10201 cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta tgatgttggt
    10261 atggaagatg cgatatcggg cagcgccatt aaagatgacg atgcatttag cgattcacag
    10321 gcggatcgcg gtctatacct taaaggatat gccgaagggc aaaagaaaac ttgccagacc
    10381 gattttactt atgcccgggg gctttctggt aaaagctttc ctgccagctg caataatgtt
    10441 gaaaatgcca gccaactgca tgaagtatgg cgaaaagggg ctgatgaaaa tgccagcaca
    10501 atacgattaa attaattacc cccaaaataa attaagcatg agattttact cattatgttt
    10561 tatttctcac attgatgacg accgcatgaa aggatgatat tttaatgagc attctgaaaa
    10621 aatatcgatg catttcgagc gaagatggtg aggatccctg aatgcgtttt tctcaccgac
    10681 tgttcttact gctgattctc ctgctgacgg gagcaccaat actcgctcag gaaccttctg
    10741 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
    10801 ggctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccttac
    10861 aagaaaatgc tgctacatct ttaccttatc tgcctgtcat cattcatact caacaagagg
    10921 cgataatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
    10981 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
    11041 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
    11101 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
    11161 ggaagaaaaa tgatggataa cgataattct cttcataagc gccccacgtt taaaagagca
    11221 ttacgcaaca tcagcatgac cagcatattt atcactatga cgctgatctg gctgctgctt
    11281 tccgtgacgt cagtgctaac cctgaaacaa tacgcgcaaa aaaacctggc actgacagca
    11341 gcaacaatga cttacagtct ggaagcagct gtcgtttttg ccgatggccc tgcagcaact
    11401 gaaacactgg cagcgctggg ccagcaaggc caattttcaa ctgcagaagt gcgtgataag
    11461 cagcaaaata ttctggcatc ctggcattac acccataagg aaccaggcga tactttcagc
    11521 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatagt
    11581 gaaaccattg gcgaagtacg cttaactgct cgcgacagtt caatcagcca ttttatctgg
    11641 ttttcgctcg ctgtcctgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
    11701 acccgccatt tgcacaatgg cctggtggaa gcgctgaaaa atatcaccga tgtcgtacat
    11761 gatgtgcgtt ctaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
    11821 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
    11881 ttacaggcca aaaatgcgca gcttctacgt accgcgctac atgacccatt aaccgggctg
    11941 gctaaccgcg cagcgtttcg tagcggcatc aacacgttga tgaacaattc cgatgcccga
    12001 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
    12061 ggtcatgcga cgggcgatag agtcttgatt gaaatagcaa aacggttagc tgaatttggc
    12121 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
    12181 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ttttaatctc
    12241 ccgtttgatc ttcataatgg ccatcaaact accatgacat taagcattgg ttacgcgatg
    12301 accattgagc acgcctctgc ggaaaattta caagagcttg ccgatcacaa tatgtatcag
    12361 gccaaacacc agcgtgccga aaaactggtg agataacaag gatatatcga tatgataaag
    12421 cacctgctag cccccctgat tttcacctcg ctaatactta ctggctgcca gtcccctcag
    12481 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
    12541 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
    12601 ctcccggaaa gccagcaaca aatccaaacc atggcagcca aattggcctc gacagggcta
    12661 acacatgccc gtatggatgg acacaccgac aactatggtg aagacagtta caacgaagtc
    12721 ttatcattaa aacgggcgaa tgtcgtggcc gatgcatggg ctatgggtgg acaaattccg
    12781 cgcagcaatc tcatcacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
    12841 gcccagggcc gcgccgaaaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
    12901 agcaaacaga caaaaaaagg gccagccaat tggccagccc ttcttaacag gatgtcgctt
    12961 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
    13021 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
    13081 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
    13141 tgaatgcaga gtgcagaccg cggttacgaa ttgcgataac cacgccctcg aatgcctgca
    13201 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
    13261 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
    13321 tttaatctct tatcctgggt aaactgatat ctcgggggct tacgccatcc catcatgttt
    13381 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
    13441 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
    13501 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
    13561 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
    13621 gtcttccgtt gccgaggcct catgtcccag tactcccgga ataaaccggg aaacggagtc
    13681 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
    13741 ttcatcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
    13801 aatcaatttt tgattcgttg ccagctcgct gacgcccgct tgatcaagct tgcgtccctg
    13861 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
    13921 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
    13981 cacggtacgg tgccgatcat acgtgaagtc gcgaggactc cagctctgga tgctcagcag
    14041 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
    14101 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
    14161 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
    14221 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
    14281 cttaatgacg agaacgtcat tagatccggt ttccatcata tctacgactt taccgagatc
    14341 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtcaccctc
    14401 ttcaagctga ggcaactgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
    14461 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
    14521 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
    14581 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
    14641 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
    14701 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
    14761 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
    14821 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
    14881 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
    14941 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
    15001 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
    15061 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
    15121 gaaatcccgg cggcatcatc cctttcatgc ttctcatcat cttcgccatc ccgcccttct
    15181 tcattttctt catcatgcgc tgcatgtcgt cgaactgttt cagaagacgg ttaacgtcct
    15241 gcacctgcat accgcaaccg gcagcgatac ggcgcttacg cgaacctttg atgatttctg
    15301 gcttagcgcg ctctttcatc gtcatcgagt tgatgatggc ttccatacgc accagcactt
    15361 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agcttgccca
    15421 tcaggctagc catgccgccc atatttttca tctggcgcag ctgctcaaga aagtcgttga
    15481 gatcgaagcc gtcgcctttt ttcagcttgc tggctaattt ctcagcttgc gcgcggtcaa
    15541 ctttgctttc gatatcttcg atcagtgaca gtacgtcgcc catgccgaga atacgggagg
    15601 caatacggtc cggatggaat ggctccagcg cctcggtttt ctcgccaaca ccgaggaact
    15661 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    15721 ccactttggt caacactaca ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    15781 tggccgcatc ctgaccggtc atggcatcga ccacaaatag ggtttcaacc ggattaatcg
    15841 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccggcag
    15901 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacga
    15961 tatctaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    16021 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc ggcagaaacc accagtactt
    16081 tcttcttgtg cttctcgcgc aggaacttac cgagtttacc aacgctggtt gttttaccgg
    16141 caccttgcag gcccgccatc agcacgaccg caggcggttg cgcagccagg ttcagggtct
    16201 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac aattttgacg aactcctgcc
    16261 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    16321 actcacgcac taccggcaga gcgacgtcag cctccagcag cgccatgcgc acttcgcgca
    16381 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    16441 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    16501 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    16561 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    16621 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    16681 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    16741 ttatttctgc ggtcatcgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    16801 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttga
    16861 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ttgctaccca
    16921 ttgtctatgc ctttgcgctt atcaacctag cgctggcgac ctttatgccc aatgaataca
    16981 tcacccatct ggaagcaacg ccggggatgc tggtgcacat tggcttatcg cttttttcct
    17041 atgccacgct aattatcgct gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    17101 tgaagaacaa gaagctggcg tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    17161 aaatgttcca tatcacgcag attggcgtgg tgctgctaac gctcactctt tgcactggcc
    17221 tgttctacat gcacaactta tttagcatgg aaaatatcga caaggccgtg ctctctatcg
    17281 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta tcatgaagga tggcgtggac
    17341 gccgcgtcgt ctggtttaac gttgcgggtg cagtcattct gacactggcc tacttcggca
    17401 gccgaatttt tcagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    17461 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    17521 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt ctgcggcata tggcgaaaca
    17581 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    17641 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    17701 tggaatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    17761 cgtgctggtt tttgctgagg tattgccaaa aaccattgcc gcgctgtacc cggaaaaagt
    17821 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    17881 gttgctgaat gctattaccc gcatgttaat gcgcatgatg ggcatcaaaa ccgatatcgt
    17941 ggttagtggc tctttaagca aagaagaatt acgcaccatt gtgcacgaat cgcggtcaca
    18001 aatttcccgt cgcaatcagg atatgctgct gtcggtactc gatctggaaa agatgaccgt
    18061 cgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    18121 atcgattctg cgccaactct cccactcacc tcacgggcgc atcgtgctct accgtgattc
    18181 gctggacgac gccatcagta tgctgcgtgt gcgtgaagcc tggcgactca tgtcggagaa
    18241 aaaagagttc accaaagaaa ccatgctacg cgccgcggac gagatctatt ttgtcccgga
    18301 gggtacgccg ctcagcacac agttggtgaa gtttcagcgc aacaaaaaga aagtcggcct
    18361 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gtcgaagata ttctcgaaga
    18421 gattgtcggc gatttcacca cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    18481 aaacgacggg tcggtgatta tcgatggcac cgccaacgtg cgagaaatta acaaagcctt
    18541 taactggcat ctaccggaag atgatgctcg tactgttaac ggcgtcattc tggaagcgct
    18601 ggaggagatt ccggtagcag gcacccgcgt gcgtataggc gagtacgata tcgatattct
    18661 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtaaaac cgctgcgcga
    18721 gagtgtggcg gagtaaaaaa acggcccggc attcgcatgc tggaccgtga tttattacga
    18781 aaacagaaat taagcttttg ccttcgctac agtcaccatc gccgcacgaa ttgtacgacc
    18841 gttcagcgta taacccttct gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga
    18901 ttccaccatt gcgatggcct gatgcacatt cgggtccagt ggtacgttag tttcggcgat
    18961 cacttcaacg ccaaacttac gcacaacatc cagcatcgac ttcagcgtca gctcaatacc
    19021 ttcaaccatc gcggacatat ccgggttagc tttatcagcc acttccagcg cacgatccag
    19081 gctatcaatc accggcagca attcgttgat gaatttctcc agcgcgaatt tgtgagcttt
    19141 ttcaatatcc agttcagtac gacgacgcag gttttccatt tcggctttta cgcgcaaaat
    19201 gccgtcacgt tcacgggtct gggcttcagc cagctgagct tcgagattcg caattttttc
    19261 atcgcgcgga tccacctgct cagcagaagc ttctggctca actgcctcaa tctcttcgtg
    19321 ctgatccatg ataatttctt ccggggcttg cccctcaggc gttttctgtt ctttactact
    19381 catgaatttc tccgcgtttt tttcgcattc atctcgctaa cttcgcttat tatggggatc
    19441 agtttcaggg tttcaaggga agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg
    19501 gaaaaatgaa taatcatttc aagtgtattg gcattgtggg acacccacgg caccccactg
    19561 cactgacaac acatgaaatg ctctaccgct ggctgtgcac aaaaggttac gaggtcatcg
    19621 ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg
    19681 agattgggca acaggctgat ctcgcggttg tcgttggcgg cgacggtaat atgctgggcg
    19741 cggcgcgcac gctcgcccgc tacgatatta aagttattgg aatcaaccgt ggcaacctgg
    19801 gtttcctgac tgaccttgat cccgataacg cccagcaaca attagccgat gtgctggaag
    19861 gccactacat cagcgagaaa cgttttttgc tggaagcgca agtctgtcag caagattgcc
    19921 agaaacgcat cagcacggcg attaacgaag tggtacttca tcctggcaaa gtagcgcata
    19981 tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt ttcgcagcgt tccgatggac
    20041 tgattatttc gacgccaaca ggctccaccg catattccct ctctgcgggc ggtcctattc
    20101 tgacaccctc tctggatgcg attaccctgg tgcccatgtt cccgcatacg ttgtcagcac
    20161 gaccactggt cataaacagc agcagcacga tccgtctgcg tttttcgcat cgccgtaacg
    20221 acctggaaat cagttgcgac agccagatag cacttccgat tcaggaaggt gaagatgtcc
    20281 tgattcgtcg ctgtgattac catctgaatc tgattcatcc gaaagattac agttatttca
    20341 atacattaag caccaagctc ggctggtcaa aaaaattatt ctaattttac gccagcctct
    20401 ttactgtata taaaaccagt ttatactgta cacaataaca gtaatggttt ttcatacagg
    20461 aaaacaacta tgttggcaca actgaccatc agcaactttg ctatcgttcg tgagcttgag
    20521 attgattttc atagcggcat gaccgtaata actggcgaga ccggcgcggg taaatctatt
    20581 gcaatagatg ccctcggcct ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc
    20641 ggcgctgctc gtgccgacct gtgcgcccgt ttttctctga aagatacgcc agcggccctg
    20701 cgttggctgg aagaaaacca gcttgaagac gggcatgaat gtttgcttcg tcgcgtaatc
    20761 agcagcgatg gtcgctcccg tggtttcatc aacggtacag ctgttcctct gtcacaactg
    20821 cgcgaactgg gtcagttgct gattcagatc catggtcagc acgctcatca gttactcacc
    20881 aaacccgagc accaaaaatt cctgcttgat ggctatgcca atgaaacctc tcagctccag
    20941 gaaatgaccg cacgttatca gttgtggcat caaagctgcc gtgacctcgc gcatcatcag
    21001 cagttaagtc aggaacgcgc cgcccgtgcg gagctgctgc aataccaatt aaaagaactt
    21061 aacgaattta atccgcagcc tggagagttt gagcaaatcg acgaagagta caaacgtctg
    21121 gcgaacagcg gtcaattgct gaccaccagc cagaatgcat tggcattaat ggctgacggt
    21181 gaagacgcaa atctgcaaag tcaactttac acggctaaac aactggtgag cgaattgatt
    21241 ggcatggaca gcaaactgtc cggcgtactt gatatgctgg aagaggctac catccagatt
    21301 gctgaagcca gtgatgaact acgccactac tgcgatcgtc tggatctcga tcccaaccga
    21361 ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc tggcacgtaa acatcacgtc
    21421 agccctgagg cattgccaca gtattaccag tcgctactgg aagaacagca gcaactggac
    21481 gatcaggccg actcacaaga aacgctcgcg ctggcggtaa cgaaacatca tcagcaggca
    21541 ctggaaacgg cgcgcgcgtt acaccagcaa cgccagcatt atgcaaatga gttggcacag
    21601 ctgatcaccg atagtatgca tgcactctct atgccacatg ggcagtttac gatcgatgtt
    21661 aaatttgacg agcatcacct gggcgctgac ggcgctgatc gtattgagtt tcgggtaacc
    21721 accaacccag gtcagccaat gcagcctatt gccaaagtcg catccggtgg tgagttgtcc
    21781 cgcatcgcat tggcaatcca ggtcattacc gcgcgtaaaa tggaaacccc agcactgatt
    21841 tttgatgaag tggatgtagg gattagcggc ccaacagcgg cagttgtcgg caaactgcta
    21901 cgtcaacttg gcgaatcaac tcaggtgatg tgtgttaccc acctgccaca agtcgcggga
    21961 tatggtcatc aacactattt tgtcagcaaa gaaaccgatg gtgcgatgac agaaacgcat
    22021 atgcaatccc tggataaaaa agcgcggtta caagagttgg cgcgcctgct tggtggcagt
    22081 gaagtcacac gtaacacact ggcgaatgcg aaagaactgc ttgcggcgta aacttttttc
    22141 ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac cggaaagcaa aaggttttaa
    22201 agtgatgaaa ggtctattat catcggcata ttacagatga gccacgtact gctcgggccc
    22261 gaaaaggaat caaatcacta tgcgctgtaa aacgctgact gctgcagcag cagtactatt
    22321 gatgttgacc gcaggctgtt ccactctgga gcgagtggtt taccgtcctg acatcaacca
    22381 ggggaactat ctgaccgcta acgacgtatc caaaatacgt gttggcatga cgcaacaaca
    22441 agttgcgtac gcattgggta caccgctgat gtccgatcca tttggtacaa atacctggtt
    22501 ctatgtcttc cgccagcaac ctggtcatga aggtgtaacg cagcaaacgc tgacgctgac
    22561 ctttaacagt agcggtgtgt tgaccaacat tgataacaaa cctgcgctga gtggtaacta
    22621 ataataaagt tgctctcaaa gacgttaaaa aaggtgctca atgagcacct tttttctgtc
    22681 tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg
    22741 agaggacgat aaatctccac ccgatcgcca tcatgcacaa tatcgctgag ttttgccgga
    22801 cggctgtaaa tgccgacttt gtttttagtt aaatcgatat cggtacgcaa ttccagcaaa
    22861 ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct gcaacgtcac gcgctgcagg
    22921 tactgcttct caggtagcgc ataagccacc tcaacggcaa ttttacctgg cactgtagac
    22981 ctcttttgca cgaacagtaa aagcctgcac catattagct gccagctctt taaacacacg
    23041 accaaaggcg agttcaatca acttattggt aaactcaaag tcgagatgaa actcaatacg
    23101 gcacgcgtcc tggctcaacg gcgtaaactt ccaaccgcct atcaatttct taaacggtcc
    23161 atcaaccaga ctcatgagaa tactttgatt actggtcaac tggttgcggg ttgtaaacgt
    23221 tttgctgatc ccagccttag acacatctac cgcagcggtc atctgcccag gagtggactc
    23281 cagaatccgg cttccagtgc accctggtaa aaactgagga taagactgaa cgtcattcac
    23341 taactgatac atttgctccg cgctgtaggg taccagtgcg gtccggctaa tctgaggcat
    23401 aacaatttcc atcaacaaaa atccaacaaa taatatcatt tatcctgctg taaaaaaaac
    23461 gctatcccgg cgctgggtaa catcgggatc atgctaagat agagccttgt cccccgcagg
    23521 attgatatgg ggtgttttcg atttcagatt accgatgatt cacgacgctt atgacgaaga
    23581 aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat
    23641 actttatcga agaagagttc gaagcgggac ttgccctgca aggctgggag gttaaatccc
    23701 tgcgtgcagg aaaagccaat atcagcgaca gctacgtcct tctgcgtgac ggagaggcat
    23761 ttctgtttgg cgctaacatc acgccaatgg ctgtggcctc cacgcatgtg gtgtgcgatc
    23821 ctacccgtac ccgcaagtta cttctcaacc agcgcgaact ggactcattg tacggtcgcg
    23881 taaatcgaga aggctatacc gtagtggcgc tctccctgta ctggaaaaat gcctggtgca
    23941 aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga taaacgttca gatatcaaag
    24001 aacgcgaatg gcaggtggat aaagcgcgta tcatgaaaaa cgcccatcgt taaacctata
    24061 taagcgatac taatcaaatc ctcaccgcgc ctccctatcc ggtggcgcga atgaacatct
    24121 tgtcgactgt caaatctgac acagatgtag ccatcccatt gcttaatcga aaaaaaatca
    24181 tactatgcag gcgctaattc caggatgata acgccttttg ggctagtcat gctgctcata
    24241 aatctggtat acttaccgtt acacattggg gctgattctg gattcgacgg gatttgcgaa
    24301 acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa
    24361 acgacgaaaa ctacgcttta gcagcttaat aacctgctta gagccctctc tccctagcct
    24421 ccgctcttag gacggggatc aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg
    24481 cctggggttg aagcgttaaa acttaatcag gctagtttgt tagtggcgtg tccgtccgca
    24541 gctggcaagc gaatgtaaag actgactaag catgtagtac cgaggatgta ggaatttcgg
    24601 acgcgggttc aactcccgcc agctccacca aataatgatt ggacggtgta aggactacac
    24661 caacaaaaac aggaagttat aagtctcagc aggacaccga ccagacggtg aggagacaaa
    24721 aaaggatacg caacggagtc gcgactccct atgacattaa agcccgctga tgcgggcttt
    24781 tttattggtc ctgacccgcc ctgaatagaa ttcatccaat tacagtccag acatatacag
    24841 tatccctcag ctcagtatca gcttctcgac aaatggtcac tgttatttac ccgtttccag
    24901 aaagagaata atttgccccc atagccagcc ttcccggaat ctgtatgttc attaatattg
    24961 ttatcgatgc catcaagtaa cataggttgg atgcgattga tcttactcac attagtcgac
    25021 acggaactca gtaagttgaa ctctgaaaaa gcagtttaaa tagcatttaa acaaatgcca
    25081 ccagttcgaa aaagagtata agcacactta atatattgtt ttaattattc at
//
LOCUS       DAPQZD010000061        24987 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPQZD010000061 DAPQZD010000000
VERSION     DAPQZD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.061"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            197..2116
                     /gene="ygfT"
                     /locus_tag="RO454_002429"
     CDS             197..2116
                     /gene="ygfT"
                     /locus_tag="RO454_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDY0934248.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            2116..2604
                     /locus_tag="RO454_002430"
     CDS             2116..2604
                     /locus_tag="RO454_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDY0934249.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(2640..4007)
                     /gene="ghxQ"
                     /locus_tag="RO454_002431"
                     /gene_synonym="ygfQ"
     CDS             complement(2640..4007)
                     /gene="ghxQ"
                     /locus_tag="RO454_002431"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDY0934250.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4043..5359)
                     /gene="guaD"
                     /locus_tag="RO454_002432"
     CDS             complement(4043..5359)
                     /gene="guaD"
                     /locus_tag="RO454_002432"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDY0934251.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(5377..6777)
                     /gene="xanQ"
                     /locus_tag="RO454_002433"
     CDS             complement(5377..6777)
                     /gene="xanQ"
                     /locus_tag="RO454_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDY0934252.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGISYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(6942..9812)
                     /locus_tag="RO454_002434"
     CDS             complement(6942..9812)
                     /locus_tag="RO454_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDY0934253.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(9809..10588)
                     /locus_tag="RO454_002435"
     CDS             complement(9809..10588)
                     /locus_tag="RO454_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDY0934254.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(10639..11967)
                     /gene="ssnA"
                     /locus_tag="RO454_002436"
     CDS             complement(10639..11967)
                     /gene="ssnA"
                     /locus_tag="RO454_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDY0934255.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFTRQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIVLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(11970..15068)
                     /gene="ygfK"
                     /locus_tag="RO454_002437"
     CDS             complement(11970..15068)
                     /gene="ygfK"
                     /locus_tag="RO454_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDY0934256.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKSKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(15390..15968)
                     /gene="mocA"
                     /locus_tag="RO454_002438"
     CDS             complement(15390..15968)
                     /gene="mocA"
                     /locus_tag="RO454_002438"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDY0934257.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            16072..16842
                     /gene="yqeC"
                     /locus_tag="RO454_002439"
     CDS             16072..16842
                     /gene="yqeC"
                     /locus_tag="RO454_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDY0934258.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            16890..18515
                     /locus_tag="RO454_002440"
     CDS             16890..18515
                     /locus_tag="RO454_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDY0934259.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLHQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(18556..19488)
                     /gene="arcC"
                     /locus_tag="RO454_002441"
     CDS             complement(18556..19488)
                     /gene="arcC"
                     /locus_tag="RO454_002441"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDY0934260.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(19536..20921)
                     /gene="hyuA"
                     /locus_tag="RO454_002442"
     CDS             complement(19536..20921)
                     /gene="hyuA"
                     /locus_tag="RO454_002442"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDY0934261.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(20974..22185)
                     /locus_tag="RO454_002443"
     CDS             complement(20974..22185)
                     /locus_tag="RO454_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDY0934262.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(22243..23439)
                     /gene="dpaL"
                     /locus_tag="RO454_002444"
     CDS             complement(22243..23439)
                     /gene="dpaL"
                     /locus_tag="RO454_002444"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDY0934263.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(23497..24684)
                     /gene="ygeW"
                     /locus_tag="RO454_002445"
     CDS             complement(23497..24684)
                     /gene="ygeW"
                     /locus_tag="RO454_002445"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDY0934264.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         6036 a         6251 c         6491 g         6209 t
ORIGIN      
        1 gcatttaatc gtttttatct ggatagcatt cttttgatcg gcaatgttga aataatgaaa
       61 gcggcataga ttgaggggca ataatttgaa ctcagttgca aaattataat taaaacggtc
      121 atgctaccta atatatgccc gtttattgca tcgcgaaatt tgagaagaca taactcatta
      181 aatgaaaggg atgcaaatga ataagtttat cgctgctgaa gctgcggaat gtataggctg
      241 ccatgcttgt gaaattgcct gtgcggtggc acataatcaa gaaaactggc cgctgagtca
      301 cagtgacttt cgaccgcgta tccacgttgt agggaaaggc caggctgcga atccggtggc
      361 ctgccatcac tgcaacaatg ccccttgcgt tacggcttgt ccggttaatg ctctgacttt
      421 ccagtccgat agcgtacaac tggacgagca aaaatgtatt ggttgtaaaa gatgcgcaat
      481 cgcttgcccc tttggcgtcg ttgagatggt cgatacgatt gcacagaaat gcgacctttg
      541 taaccagcgc agttccggca cgcaagcctg tattgacgtc tgcccaacac aggcgttacg
      601 gctgatggac gataaagggt tgcagcagat aaaggtggcc cgccagcgca aaacggcagc
      661 aggaaaagcg tcatcagacg ctcagccatc tcgcagtgca gcgttgctcc ccgttaactc
      721 gcgtaaaggc gcagataaaa tttcagcgag tgaacggaaa acccactttg gcgaaatcta
      781 ttgcgggctg gatccacaac aagcgactta tgagagtgac cgctgtgttt attgtgccga
      841 aaaagctaac tgcaactggc attgtccgct gcataacgct attccggatt acatccgtct
      901 ggtacaggaa ggaaagatta ttgaagcggc agaactttgc caccagacca gttccttacc
      961 cgaaatctgc ggcagggtat gtccacagga ccgtctttgt gaaggtgcat gtactttgaa
     1021 agatcactct ggcgcagtct ctatcggtaa tctggaacgc tacatcaccg atactgcgct
     1081 ggcgatgggc tggcgtcccg atgtcagcaa agttgttccc cgtagcgaaa aagtggcggt
     1141 gattggcgct ggacctgcag gattagggtg tgctgatatt ctggcgcgcg cgggagttca
     1201 ggttgatgtc tttgatcgcc atccagaaat tggcggtatg ctgacttttg gcattcctcc
     1261 tttcaaactc gataaaacgg tattaagcca gcggcgagag atattcaccg caatgggaat
     1321 cgatttccat ctcaactgtg aaattggccg cgatatctct ttcaacgaat taacggcgga
     1381 atatgatgct gttttcctcg gcgtggggac ttacgggatg atgcgagcag atctgccgca
     1441 tgaagatgcg cctggtgtca ttcaggcgtt accgttcctg accgcccata cccgccagct
     1501 catgggattg ccggagtctg cagagtatcc gctgacggat gtggaaggta agcgagttgt
     1561 ggtattgggc ggtggcgata cgacaatgga ttgtttgcgg acttccatcc gccttaatgc
     1621 tgccagcgtg acctgtgcgt atcgtcgtga tgaagtcagt atgccgggtt cgcgcaaaga
     1681 ggtggtcaat gcccgcgagg aaggtgttga gtttcagttc aatgttcagc cgcaatatat
     1741 cgcttgtgat gaagatggac gcttaacggc ggtgggcctg attcgtaccg ctatgggtga
     1801 gccggggccg gatggccgcc gtcgtcctcg tccggttgcg ggttcagagt ttgaattgcc
     1861 cgccgatgtt ctcattatgg cctttggttt ccaggcacat accatgccgt ggttgcaggg
     1921 cagcggaatt aaactcgata aatggggcct gattcaaacc ggcgacgtcg ggtatttacc
     1981 cacccagacg catctgaaaa aagtctttgc tggtggtgat gcagttcatg gcgcagatct
     2041 ggttgtcact gcaatggccg caggaaggca ggcggcgcgc gatatgttaa ctctgtttga
     2101 tacgaaggca tcgtgatgaa atcgttaatt atcgttaatc cggctgactg tattggctgc
     2161 cgtacttgtg aagtggcctg tgtggtcgct cacccttcag aacaggagtt gaatgccgat
     2221 atctttttac cccggctgaa ggtacaacgg ctggatagca tcagtgcgcc ggtgatgtgc
     2281 catcagtgtg aaaacgcccc ttgtgttggc gcttgtcccg tgggggcgct gacgatgggc
     2341 gagcaggtgg tgcaggcaaa ttctgcccgt tgtattggct gtcagagttg cgtcagtgcg
     2401 tgcccgtttg gaatgatcac cattcagtca ttgccggggg atacccggca acaaatcgtg
     2461 aaatgcgatc tctgtgaaca gcgggaagag gggccagcct gcgttgaatc ttgcccgaca
     2521 caggcgctgc agttgctgac tgaaagagaa ctcaggcgag tccgccagca gcgtattgtt
     2581 gccagcagtg agaatcctct gtgaaagaaa tctccgcgct gaaaggcgcg gagaatctat
     2641 tagattgccc aaccacccgc gtaaaacgcg accagtgcgg cagtaatgat caccgtacca
     2701 atattcagct tttgccattc gcgcgcaaag acgcgaccca ccaccagtgt tacaaagccc
     2761 agcataatac cggtaacgat attacaagtc agaacgatga acacggcaca caccaggcca
     2821 gccattgcgt caataaaatc gttgaaatcc agcttcgaga cgttgcttaa catcagcaaa
     2881 cccacgtaca tcagtgcggg agctgtggca taaccgggga tcagaaacga taacggtgat
     2941 aaaaacagaa tcatcaggaa caacacacca accacggttg cggttaaacc ggttttcccc
     3001 ccggcggcgg ttcctgccgc tgattcgata taaaccgccg cgggcgctgc gcccaccagg
     3061 ccggagaata ttgaacttac tgagtcactg gtcagggctt tgccgccgtt gatgatctgg
     3121 ttgtctttat ccaacaaatt cgcctgaccg gcgacggcac ggatggtgcc agtggcgtcg
     3181 aacactgcgg tcatcaccaa tgccagtaca ctcggaagta cagttggctg gagtgcgccc
     3241 ataatatcga ggctgaaaat cagagactta ccatcttcgc cagtcaggct tggcatcgcc
     3301 accagaccgt ggtacttcac cgctgggtca aagattaagc cgatgatcga aattgcaata
     3361 atcaccaaca agatcccgcc gggtacgcgg cacttttcca gaccgaagat gaccgccagc
     3421 cccagtaagc tcatcatcac cgggaaggag gtaaaggcac cgagcgccac tggcaagcct
     3481 tcaatcgggt tttttataac cattcccaca ccgttagcag caatcagcag cagaaacagg
     3541 ccgataccga tacctgtacc gtgagcgatc cccatcggca aattacgtaa gatccaggta
     3601 cgcacaccgg ttacggaaat agcggtgaag atgacgccca tcagaaagac tgcgcccagt
     3661 gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa cgccgtcaag
     3721 gaaatagcgc aaccaatcgc cattggcaaa ttggcccata accccatcag caacgagccg
     3781 aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc
     3841 agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc
     3901 agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg
     3961 ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca
     4021 cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg
     4081 tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag
     4141 aaacagagtt gtcatagcgc agctgctgta gcggagtggc agtgggttcc atcaccacga
     4201 aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg
     4261 ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt
     4321 gcaatacctt gtaggcttcg ttcagcgttt gcagcatgtt aaaagttgtt ccggcaccga
     4381 tatccgttcc catgcccact ttgactttct tctgccatgc ttttttcaag ttgaataagc
     4441 cgctgccgag gtaaaggttg gaggtcggac agaaagcaat gctggatttg gtttcgctga
     4501 gacgatccca ctctttttct tcgagatgga cgcagtgagc aaagacgcag tttttaccgg
     4561 tcaggccgta ctgatggtaa acatcaagat aaccatcatg gtcaggataa agcgatttca
     4621 cccaggcaat ttcatctttg ttttcacaga gatgggtatg tacccatgta tccggatatt
     4681 cttctttcag gcgttgcgcc atcgccatct gttcaggaga tgaggtaggg gcgaagcgtg
     4741 gcgtaatcgc atacaacaga cgaccatttt tgtgccagcg ttcaatcagt tctttgcttt
     4801 ggtgatagct gctttcggca gtgtcgagca gataatccgg tgcgtttcgg tccatcatca
     4861 ctttaccggc aatcatacgc atattgatat gactggcggc ttcaaacagc gcatcaacgg
     4921 attgtggatg aacagtgcca aacaccagcg ccgtggtggt tccgttacgt aaaagctgct
     4981 tgatgaagaa cgccgacatc tcgcgggcgt actctaaatc ctcataacga cgttcagtag
     5041 ggaaggtgtg tttattcaac cactccagca attgttcacc ataggccccc accatttcac
     5101 tttgcggata atggatatgt gtatcgataa agcccggtac tatcagtttg ccgcgatagt
     5161 cgcgcacgcg aatggtgtca ggaatttgat gctttccgtc ttcccattcg ccaaaccatt
     5221 ccacttttcc ctgtttaatg agtaataaac catcctcaat aaaccgcagc gcagaggcaa
     5281 tttcttccgg gttatcgacc gtacgggtga catcaataaa actgcctcgt accgctttta
     5341 acgtgtgttc tcctgacatc attgactcct ttactgttaa tccatctctt ccgctgaggt
     5401 aataccaggc agaacgtttt cctgtcggta gccaccaggg aggataatat ttaataaaat
     5461 cgcagttaac ccgccagcac aaatagggtt ttcaactaat acataaatag aggctggcaa
     5521 tattttaaaa atttcgggat cgtaggagat accaagccct aaacctaaag aagtggcaac
     5581 aataagcgtt tcacggcgct ttaaaccgtt ggtgatgatg atgcgaatcc ctgcgatggc
     5641 aatcatggaa aacatcaacg tcattgcgcc tcccagaact gctgagggaa tggtcgtgaa
     5701 gaagccgcca atcatcggaa ataagccgag gataaccagc attaccgcga tggttcgccc
     5761 gacataacgt gaagcgacgc cagtcatctg aataacccca ttattttgcg caaacgtggt
     5821 taatggtaat gaaccgacag cggaggcgat aacagaaacc agaccatctg ccagcacgcc
     5881 gcctttcagt cgggactgat actcttcccc ctgaatggga cggcgggaaa ccattgccgt
     5941 ggcggtgata tcgccgacag cttccagcac gctaagcaga taaatcgtgc caaccaccag
     6001 gaactgatgg aagctaaaac taaagccgta tttgaacgga tgcgggatgg tgattaacgg
     6061 caaattgcgc atactgctga aatccaccat gcccaggcat aacgatgcga tatagccgac
     6121 acatagccca atggcgatcc ctcccatgcg tagcaacgga ctgcgacagc agttaaagcc
     6181 gatcaccaca atcaaaacca ataaaccaac gccgagatgt tcgtaattgc cgaacgtacc
     6241 gctgcttttg gctgcaaatc ctccaccaaa atcaataatg ccgactttaa tcaggcttaa
     6301 gccgatcatc agcaccacaa tgccgctgac ggtaggcgta ataacccgac gtaaataggg
     6361 caggataaaa gaagagccga caaccagaaa tgcgccaacg aaggagacgc cgaggagcga
     6421 cgacatgatt aactcttcgt gaaaaccgtc gcttttcatg ctgctgccca gcgcaatcat
     6481 gaccgtaaca aatgaaaaat tgactgactg aattgaaagt aaaccagaac cgacgatgcc
     6541 gtagcggttt acttgtaacc aggtaccaat accagaggcg atcatcgcca tagaaacaag
     6601 ataggcagtt gtttcagcgg aaagctgtaa ggccgcaccc acgattaacg cgggggttac
     6661 catcggaacg aaaattgcca acagatgggt aatggcacct acgagagcct gatgaaaggg
     6721 agggcgatcc tccagttcaa atataaggtc agaacctgca tggtttatat cagacatccc
     6781 ttcactcctt ttaaagtaaa tttgatcatc tgaattagta aaataaattc ccacgaattt
     6841 ggggcgcagg acgccttgag aacggcttat ctcattttcg cagtcactat ttagtcatcg
     6901 ttcaaacaga gaaacaggcc gccgaagcgg ccatttatta cttatatttt ttccagcgcg
     6961 gtgagtattt tctccggtgt gaaatgccat tcgcgtaacc agatgccgca ggcatcgtga
     7021 attgcggtag caatcgccgg agctgcgcca tttacaccga tttccgagat cgatttcgcc
     7081 ccgaatgggc cgactttatc gtcgctcggc accagcacag cgcggaaatc acgcggaatg
     7141 tcaccaattt tcggtgcgcc gtaactgcgt aaatcacgcg ttaacgggtg accttcggcg
     7201 tcataaatga tctcttcgct catactgtgg ccgatagcac gcagggtggc accgtagatt
     7261 tgtcccaacg ctaactctgg attgaccggt gtaccgcagt ccagcaaggc gtagaattta
     7321 tccaggcgga tttcacccgt acgcgtgttg acggcaactt cagcgaagtt tgcgccatac
     7381 gggaaggcga aatcaggtgt gatataactg ccagttccca ccagtgaacc aaagccggtg
     7441 ccggtttcgc ctttatgggc tatctctccg aaactaactt cgcctttctt gccgcgaacg
     7501 acgcccggcg ttgctagttg aacatctgcc actggctcac ccaatatttg cgcgccgtgg
     7561 aacaggattt tctcccgcag attttccgct gccaaacgcg cagcgttacc cgagaagcaa
     7621 gtaccggacg aggcatatgc gcctttatca aacagtgcat gatcggtatc accagagata
     7681 acatgcacgt cctgcggtgg gcagtgcagc acttctgctg ccagtttggt caccacagta
     7741 tccagaccag taccaatatc cgcaccaccg gaatgaacga taaaggtgcc atccgattcc
     7801 agtttgatca tgcagttagc ctgatcgata tccgggatac ccgatttctg catgataatg
     7861 gcgacgccgc gaccgatgtg ccagtcacca ttttgcggtt ttggtgaaga ccattggatc
     7921 atctcgcgac cctgacgcag gatctcttcc agtgcgcagc tggcggcgga aggaacggag
     7981 gtcggcgctt taccttcacc gattgcaccg agaattttca gctcttgccc ttcgtgtacc
     8041 cggttacgtt cgataatttc cagttggtcg atctgtaact gttcagccag ttccgctaat
     8101 gccatggtga tagcgaagtt acctttcggt gcgccataac cctgataagc accatttggg
     8161 caaatgttgc tgtagtaggt ggtgacctga aaatcgacgt tatcgcatgg atataacggc
     8221 agcgacagcg ccggtccgtt acacggtacg gtgagtgagt ggttaccgta aggacccgtg
     8281 ttggcgcgga aatccatctt cactgccgtc aggcgaccat cttttttcgc gcccagtttg
     8341 acggtgactt tcgcgacgtg acgagaggtg ttagcaataa actcttcttc acgggtgtag
     8401 cggaaaagta ccggacgccc ggtcacgcag gttgcccagg cgcacacttc ttccagcagg
     8461 atgtcctgtt tggaaccaaa accgccgcca actcgctctt taatgacatg aactttatgc
     8521 tgtttcatgc ccacgaggcg cgcgacctgg cggcgtaagt gccatggtac ctgggtggag
     8581 gcgtggatca ccagacgatc gccgtccata cgggtaaagc agatatgtgt ttcagtcggg
     8641 cactgctgtg cctgcgttga attataggtt cgctcaatga tcacatcggc atcggcaaag
     8701 cctttgtcca tatcgccaat atgaccatga atactggcgg cgatattttt gcgtgggcga
     8761 gaaccgatcg ggaagttgat gatcatatgc tcgccgcgct gggctgcatg gctgttatca
     8821 tcttccagag tatctggcgc accagcaaca tacaccaccg gttcatcgtg cacgacaggc
     8881 gcatcttccg ccattgcctc gtcaatcgac attaccggct taagcacttc atattcaacg
     8941 tcgatgagct tcagtgcttc gagcgcaatg tcttcacttt cggctactac cgcagcaacg
     9001 cgatcgccga cgtgacgcat tttcttgccg aacattcggc ggtcaagcgg tgacggttcc
     9061 ggtgcgctct gaccacccgg ggtatagtaa atatccgggc aattcaggtg agtaataacg
     9121 tgaacgacgc ccggtaaggc ttcagctttg ctgacatcca gatgagtaat cagtgcgtga
     9181 gcgtgtgggc tacgtaacat tttaatgacg caggcgtcag ccgttacgcg atcttcaaca
     9241 tagcagggtt tcgcctgcac cattttcgcg gcatcagttt taggataatg cttgccaatg
     9301 acttcaaggt cgtcacggaa agtcggagcg atatcaatgg tggcctgcgg attattttta
     9361 cgtgcaaccg ccagttcaat gacctgatag tattgctgcc agccagcatc gcggctaaac
     9421 agacctgaga gtgcgtcgtc gatctcttcg cgggtaggtg cgtcgatgcg atcgagaaga
     9481 tcggtgataa tcagagccgc agctggatcg ttataaccag actgcaccac gccaacatca
     9541 accatggcct gttgaaccag acttaactca ttccatttgc ccagagattc tgcggtacga
     9601 atatctgcct tctctaactg tgcggcgata agcaaggacg cgttaacgat attaccgtta
     9661 aagataattg cgtcagaacc ggcaaaccca aagccatcat cactgttgcg tacagagtgc
     9721 attcccatgt taaacaacag cttttgcacg ttttcgcctg gattaacggt tagctcctga
     9781 ggcgcgccat ttaaagtaaa gtggatgatc atacggcttc ctccccagcc tgttggcagt
     9841 cggcatacag gtcggctacc agaactcccg tgatatagcg tttataggcc acgctgcccc
     9901 gcaaatcttc ctgcgggaaa atggcgttgg cgacagcctg ttccagtgca ttgccttcca
     9961 gattttgagt ttcaacatca tgcagacgca gtgctttact ggcgacgcca tccagcgcaa
    10021 ttcgcatacc gtcgtggtct gttattgcaa cggctgccgt cacgacggtt aaacctgcct
    10081 gagaacggct aattttgcga gtcgcacagg tgcgatacgg atctttaatg ataatttcgg
    10141 ttaacaggcg atcgcatggg caggccaggt agtcctcgat tgacagcgtt tcgccgttgc
    10201 caaaaaccag ttcagcatcc agtgccagca ggacgggaag cagcactgac tcttcttgcc
    10261 gggcggcaat ttcaccacca atggtcgact gattacgaac atggcgagag tagacaaaac
    10321 cgagagcttc ccgcagtgct gcaggaataa atcgcgcatc acgcagtggc tgcaagcgag
    10381 acattgcgcc aattcgtagt gcaccgttat cccagtcaat ccagtccagc tccagatcct
    10441 gcaaggaaat ggcaatcttt ttatcggtac gggttggtgt agcgttgagt ttgctgcccc
    10501 cggcgaacca gacggcttca tcctggtagc ggcgcttcag ttccagcgcc tgttcgacag
    10561 agtcgggtct gaaaaattgt tcaatcatcg tctttcctta atttcccctc ttctctgaaa
    10621 gaagagggga gctgttattt atgccagcgc atccatccga cgccacatac tggcagcggc
    10681 ttttctggct tgcgcataaa tggaatcgca atcgaagtta aactgacggt cttcatagac
    10741 catcacacca ttgaccatca cgctgtgaac gctacctgag cccataccga aagcgatatg
    10801 cccggcgata ttgtctgcca gcagcggcgt cggcgagttg taatcgcaaa tggttaaatc
    10861 ggctttgtaa ccggcttcca gaagcccaaa tttcgcgcca aagttgcggc tcatcagttc
    10921 gttgccgtta gccagtgctt tggcaaaact gtcaggccac agcgggccac ccgcatcgcg
    10981 atgtttaaag aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc cgtccgttcc
    11041 caacgccaga ttgcggatgt cgctaaggtg atggttgtag ccgacatggt tgttcatgtt
    11101 tgaacgggcg ttatgcacca ggaacgcatc gcgctgattg agtaggacga tgtcatcttt
    11161 cgacaggtac agcccatgag cgaccagcgt tttgctgtcg atgagatcga attgcgcaag
    11221 tcgtgccagc aggtctttgc cgtaccaatg gtgactgtag gaaacgtcgt aaaggtcttc
    11281 cgcaacgtga atatgcaaac cacgacctgt gcttttcacg gcttcacgca gcatctccag
    11341 accggcatcc ggcacggtaa acggcgcgtg agcgccgata tgtgcttcca ccagatacgg
    11401 ctcggttgct gctttcttcg cctcatcaat ctgacgggtg aagcggatgt tttcttctac
    11461 accttcctgc aactctttga tgccgttgtt acggtcagta gtttcaaaac aggtcatcgc
    11521 gcgcaggcca acttttaaaa atgcgttgcg caatgtggag agcgacccgt cgatatacgc
    11581 cggagaggca tggtgatcga taaccgatgt acatccgctc ttaatcgctt ccagggaaca
    11641 aatcagtccg ctgtaataga gcgactcttc atcaagagcg cgatcgagcc gccaccagag
    11701 atttttcagc gttgagatga aatccgggca gggggcgatg tttgccataa ttccgcggga
    11761 aagccccgag taaaaatggt tgtgcgagca gacaattccc ggcatcacaa tccgaccatg
    11821 catctctttg tagctggcat cggggtagcg ttgcgtcagg gcatcgccga tagcgacaat
    11881 cacatcattt tcgatggcga tatcaacgcc ttcctgcact ttcgccgggt gtaactgcac
    11941 tgcggtgaca ttcttcagaa tcaacatgat tacacctcca cgcggcccag cagataatga
    12001 tgatgctggt ggacatggct aatgatgcgg cacatatcgt tcagctccgg tggtacgttg
    12061 ttgaactgac cttcgctgtc gatgtttaac acccagcttt ggttattcag acgtacgcgt
    12121 acccggcaat cttccacaag gaagcctggg ttactgctgt tatcaaagtc ttgcgacagg
    12181 ctgaagacgg tgattttgtc tttgtacggt ttaccgttcc acgggcagaa ttgggcgcag
    12241 ttgccgcatt cgttgcagta ggcatcgaga tgcagcgtct ggaaacggtt ctggaagcca
    12301 ggaaccgcaa tggatacgtt ggcgcggttc gggcagacat ccacacactt gctgcaaacg
    12361 tagttacatt caaggcagcg tgcggcttcc tgtgcgacaa acgcgtcacg atcgtcactg
    12421 ttcaccagag tgacagagat atcgccttta cgttgataga tttccgccgg atttacgttg
    12481 ttccagtatt tatcgttctg gtgggaacgg atattttccc gactaaggat ggtatcggtc
    12541 gcccgacgcg cggttccgac agcagcgaca atggaggatg gtccgcgctg tacgtcgccg
    12601 atcataaaga cgtcagtcag acgcgtttcg ccattatggt cgacgtctgg ccagccgttt
    12661 ttgtccagcg gcacgcccat cgcattcagg gcttcagtat cctgctgttc accaatggcg
    12721 gtgatcagac tgtccacatg cagtgttact gtttcgttgg tttcaaccgg acggcgacga
    12781 cctttctcat caggttcgcc aagcgacata acgcgcaagg ttaaggtgcc atcagcatcg
    12841 aaacgttccg gattattcag aaaacggaac tccacgccgt cgtgcaacgc ttcttcatac
    12901 tcttcgcgcc atgcgggcat ttcttgcagt gaacgacggt aaacgaccgt tgctttttct
    12961 acgcctggaa cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc cgccccgaca
    13021 acgaccacat gtttgcccag cttgagcgcg gtgcccttgt tgtattcacg caggaaaggg
    13081 agtgatttcc agacattttg gttgtcgccc gccagtttca caccgctatt tttatcagtg
    13141 ccggtggcaa tcagaacata gtggaagccc tgatttttta actgttcaac ggtcaaatcg
    13201 ggtgagcaac cgtactcaaa tttcacgccg tgagcggcaa caaaatcgat atcgtgctga
    13261 attaactctg caggaatacg gaactgagga atgatatttt tcaccacgcc gcccgcattg
    13321 gcttcgcgtt caaacagcgt gaccggatgg cccgctctgg caaggaagta acctgcggcc
    13381 agacccgccg gacctgcacc aatcacggct accggatggc gtgaaccgga accggctggt
    13441 ttgtgccagc gttgcttata ttcatcccaa cctttttcca gggcgacttt tttcagttcg
    13501 cggatattca gcgcactgtc gtaatccagg cgggtacagt tgtattggca ctggtgatcg
    13561 caaatatgac cggtaatggc gggcagggcg ttgcgttgat agatgagttc cagcgcgtcg
    13621 gcatagcggt gttcgccaag cagacggatg tattccggaa tatcttgctt gatagcgcag
    13681 gcagtaacac agggggcaac gtagcaatcg gtcagcggca ggtcttcagc cacttcaata
    13741 cgctcttctg gcttccagtg tttctgggtg tattccatgg ttaacgcgtc tgctgccagt
    13801 ctgttcagtc gttcgacgtc aacgtggtta agctcccagg cgtcggagcc ttccagctcg
    13861 cgcatgcagg cacttaagcg cagatagccg ccaggtttca gcaggtcggt tgccatagta
    13921 atagggcgaa taccagtatc aaaaatatcg cggatagtca gctgactggc accaccggaa
    13981 taagaaatgg gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac gttgatggag
    14041 agcgggaaca gcgcacggcc tgacatatac atctcttcac cgggcagtgc gcctttattg
    14101 ttgatggtgc cgagagtgtt agtcagtttt acgccaaagc cgagtgattt ttcttttgcc
    14161 agtgccatca ggcgttccag catttccagt gcttgcgtca gcttgaggtc gtgatcaaat
    14221 gactcttctt ttaagccgat gtaaccgaaa ccgcagacat cgaggatctc acgaacacgc
    14281 gcgtacccca gtaaggtcgg gttgagtttc acaaaggtgt tgagtccttt ttcttccagc
    14341 atgtagcggc aaatggcttc gatttcatgc ggaggacagc cgtgcatggt ggagagggta
    14401 acaccttgta ccatgctggt ggggatgcga gcgggtaagg cttgcaagct ttcgcgtttt
    14461 tcctgcaatc cgtggcgggc taaaaatgcc tcatcctgga gtagcttatt aagtgcatcg
    14521 cggtattgag cgaatttcgg atggtcagat gcgtccatca tattgtcgat gaactgctgc
    14581 atcggcggtt gcttaatacc ttcgaggttg taaccgacgc tcatattaaa gatgaacgat
    14641 ttaccggaat cagaaggctg gaacatcgct tcgagaaggt gcagggcaaa ccaggctttg
    14701 aggtattcat cccaggcttt aagcagggta aattcggtag accattcggt gttaaagcac
    14761 tcgtcttcgg catcgataca gggcttttcc agctccagac ggtcaagaat ttggacggtt
    14821 tttagttcga tgaagcgtcc gccagtcagc caggaagtga caatattttg cgcgagttgc
    14881 gtgtgcgggc cagcggcagg gccgacggga gtggcacagg tttcaccgaa gacgctaaca
    14941 gttttacttt ttacgggtga gtaaaactgt tgctcgggaa taccaaagat tgagcgttgt
    15001 tgttggtatt catcaaatat gcgcgtcaaa agttcctcaa acggaatggg acgcataata
    15061 tcccccataa ccctctcctt agcttataca gatatcattg agtgaccggt gttgagaaat
    15121 ccggtggttt agggggatag agcaacaatc gcaccactct tgccaggttt gacgggaaat
    15181 ataaaaaaat gtgaggcggc tcgaaactta atggcttttt gtgaatgaac tcacaggtgc
    15241 caaatgagaa ttaaaatcac tcctgaaaat tattattgcc agcccattta tcactttttc
    15301 tcttttctca aaatcagtga ttccttatca ttatgataaa gagtgctgga gggattaaat
    15361 atttgaacgc aatcgtttaa ctacgttttc taaatttcag tatacctttt cttcgctgta
    15421 ataaagtcgt ccggagtatc aatatctaaa attatttctg cattttctat ttcgacggaa
    15481 taatgttctc ccataaggag tgcctgacgc atattggtga cattggggcg ctggattgcc
    15541 tgcatcaggc atggttttga cactaaaatc ggatggccgg ggatgccatt atggagtggc
    15601 agtattgcgc catcatttcg taacgaccag atttttctaa aaatatcgat ggtgagggtt
    15661 ggcatatctc cgtgggtgag aaaacaatgt tctgtttgca ccgcgggtac tgcggccttt
    15721 actgatgtca gtaaaccctg cgcataatct gggttgtgga taatagtgat attgctctgg
    15781 ttcgcataac gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta
    15841 caaaactgca acgcattttt gatacttgta tcaagaattg ttccctgttg ccagggtaac
    15901 atcattttcc attgccccat tcttgatgat aatcctgcgg cggtaattat acagtcgatg
    15961 gctgacatag tgattccatt atttacagga gctaataacg cctgataatt gcgttacttc
    16021 ctgatggatt acgttgaggg taatttatcc cagagaggtc cataaagacg cgtgaaaagt
    16081 ataattgacc catcggcatt attcattgat ttaggcgcgc agaaacgtcc cactgtgatt
    16141 tctattgttg gtgctggggg gaaaaccagc ttgctttttt ggctggcaga actgttccag
    16201 gcgagtggca ggcgtgtatt aatcactacg acgacacata tgtttatgcc aacatctcac
    16261 tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc atgcgtcttt tatatccccc
    16321 atttcatttt gttttcactg ctggaaagcg aaccagggaa aagtgcaggg atttacgcca
    16381 gaagcgattg atgcactggt acaacgacca gagtgtgacg taattctcat tgaggcagat
    16441 ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg aaccttgcat acctaaaagc
    16501 agttgctgcg tgattgctgt gatgggagga catattttgg gtgcgaaagt gagcacagaa
    16561 aatgtccatc gctggtcgca gtttgctgat attactgggt taacacctga tgcacccttg
    16621 caactgagcg atctcgttgc gctggttcgc caccctcagg gcgcgtttaa aaacgtaccg
    16681 caaggttgtc ggcgagtctg gttcattaac cgtttttctc aatgtgagaa tgcgattgcg
    16741 caaagcgagc tccttcaacc gctgcaacaa cacaacgtag aggcaatctg gctgggcgat
    16801 atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt agcggcccgg gtattccggg
    16861 ttactgaaag gatatccatt gaggttggta tgaatatttt cacagaggct gcaaaactcg
    16921 aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga tagccgaggc tcaacccccc
    16981 gccattctgc acaaatgtta gtgcgcgccg atggttctat cgtcggtaca attggtggcg
    17041 gaatggttga gcggaaggtg attgaagagt cgcttcaggc attgcaggaa cgtaagccgc
    17101 gattattcca tggacgtatg gctcgtaacg gtgcggatgc tgtcgggtca gattgtggag
    17161 gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc acgtctggtg ttgattggtg
    17221 cggggcatgt caaccgggcg atagcccaga gtgcggcgct attaggattt gatatcgccg
    17281 ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt cccaccatca accacgcttc
    17341 tccatgctga gtcgtttggt gcggcagtgg aagcactgga tattcgccct gataattttg
    17401 tcctgattgc cacgaataat caggatcgtg aagccctcga caaactcatt gaacaaccca
    17461 ttgcatggct ggggttgctg gcaagtcgcc gcaaggttca gcttttccta caccaattgc
    17521 gtgagaaagg cgtggctgaa gaacatattg cccgtttaca tgcgcccgtt ggttacaaca
    17581 taggtgcgga aacgccgcag gagatcgcca tcagcgtgct ggcagaaata ttacaggtga
    17641 aaaataacgc gccgggtggg ctgatgatga agccttctca tccttccgga caccagctgg
    17701 tggtgattcg cggtgcgggg gatatcgcca gtggtgtagc gctacgtctg tatcatgcgg
    17761 gttttaaagt gatcatgttg gaagtggaaa aaccgacagt gattcgttgt accgtggcgt
    17821 ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg cgtcaccgcc cgcctggcaa
    17881 ccagctctgc ggaagcgatg aaacttaccg aacgcggatt tatccctgtg atggtagatc
    17941 ccacctgttc attgcttgat gaactgaaac cgctttgcgt ggtggacgct attctggcga
    18001 aacagaattt gggaacgcgg gcagatatgg caccagtaac aatcgcgctt gggccgggct
    18061 ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa tcgcgggcac tggctcggtc
    18121 aggtgattta ctcaggttgt gcgcaggaga ataccggtgt tcccggcaat attatggggc
    18181 ataccacccg acgagtgatc cgcgctcctg ctgcaggcat tatgcgatcc aacgtgaaat
    18241 taggcgatct ggtgaaagag ggcgatgtca ttgcctggat tggtgagcat gaaattaaag
    18301 caccgttgac ggggatggtg cgtggcttgt tgaacgacgg actggccgtg gtcggtggtt
    18361 ttaaaattgg tgatatcgat cctcgtggtg aaacggctga tttcaccagc gtttctgata
    18421 aagcccgggc gattggcggc ggcgtacttg aggcgttaat gatgttgatg catcagggcg
    18481 ttaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa aatggccgga tggtattacc
    18541 catccggcct ttaaattact gacaaatcac cgttccggtt ttgccttcaa tcccttcttt
    18601 cgctttgctc aatacggtaa tcagtgcttc acgacctgcg cgagagcgag caaacgaggc
    18661 ggcagcttcc actttaggca gcatagaacc tttcgcaaaa tgaccttctt cgataaagcg
    18721 ttcagcatca cttaatgaca ggcgatcgag ccactgttca ttctcttttc cgaagttaat
    18781 ggccactttt tctaccgcag tcagaatgat cagcatatcg gcatcgatca tttctgctaa
    18841 acgggcactg gcccagtctt tatcgataac cgcgctggca ccacgcagat ggttaccttc
    18901 acgaataaca gggataccac caccgccaac ggtgatcacc acctgtccgg catctaccag
    18961 agctttaacc gtttcttttt caatgatatc aaccggtttt ggcgaagcaa cgacacgacg
    19021 atagccgcga cccgcatctt ctttcagagt gtaaccctgt tttgtcagta gctctgcttc
    19081 ttgctcggta aagaacgaac caatcggctt ggtcgggttg aggaatgccg gatcgttagc
    19141 atcgacttcg acctgggtaa ccagcgttgc tacaggttta ttaatgccac gagaaagcag
    19201 ttcttctctt aaggcgtttt gtagatcgta accaatataa ccctggctta aggcaacaca
    19261 gacagacatc ggcagcattg gcgagtgcgc ttcagttttt gcggcagctt caaaggcctg
    19321 gttaatcatg cccacctgtg gcccgttgcc atgagtgacg atgacctcat gtccctgggc
    19381 aattaaatca acaattgcct gagaagtaat ttttaccgct ttcatttgtc cggccaggtc
    19441 atcgcccagc gcattcccgc ccagggcgag aacaattttc ttactcattt ttcctcactc
    19501 atcattcttg attgtgtata gatccctggc agtggttaga gcacgggagg gacaaacggt
    19561 ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa aggtgccgtc gcagaaaatc
    19621 gtttcaccac gggataaggt tctgacaatc gcgccctgac aggtaaaacc ctcccatggc
    19681 gagtagtcag cgttgtcgtg gagatggcga tgctgaattt gttggctctg acgtgggtct
    19741 ataatcacca cgtcgccatc ggaaccgggc gctaataatc ctttttgtgg ccacaggcca
    19801 aacagcctgg cgggcattgc actggttaac tcaacaaaac gctccggtga gatacgcccc
    19861 gtcatcacgc cactggagaa cagcagttgc atgcggttct ccacaccggg taagccattt
    19921 gggcagcggc tgaaatcgcc tttagaaatt tgcaggcgtt gagccatcga gaaggtgcag
    19981 tgatcggttg ccaccacgtc aatcgcacca tcgctgatgc cacaccacag tttgtcctgc
    20041 tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca tgccatcttc tgtatcgtaa
    20101 ctgcgttcgt ccaacaggag atattgtggg caggtttcaa cccagactgg ctggtgattc
    20161 gcacgggcaa gacgcagata atccagacct aagccgttag acaggtgcac gatatagagc
    20221 ggggcgttac cggcaatttg tgccaggtta atcatgcggg cgatggcttc cgcttcgcat
    20281 tccagaggtc gactcaaggc atgataacgc ggcgcggtta accctgcggc gataaactcc
    20341 gcccgcttgc tggcgatagc tgcgtcattt tccgggtgca cggtggtcag cgcgccggac
    20401 tcatgcaagc ggcgtaatgc ctgcaacact tcatcatcgt tgagtttata ttgataggtt
    20461 aaatagagtt taaaactgct cagtccttcc tcaaccatca tcggaatttc gtcgaggata
    20521 gcgtgattaa tatgttgaat gacaccgtga aagctgtagt cgatgactgc tttgtgggcg
    20581 gcataaccgc gataaacttc cagttgatgg cgtaaccggc agccatttgg gccaaatccc
    20641 atatggtcaa taatggttgt tgtaccgcca cacgcagctg cgcgggtacc ggtaaaaaaa
    20701 tcatcacaac tgcgcgcgat gccgacatca atattgaaat gcgtatggac atccacgccg
    20761 ccagggaaaa cgtaacagcc agaggcatca atttcttcgc aagggagttg cggcgaaata
    20821 tcggtaccca actggcgaac aatcccgctt tcaatcagca aatcctgctt cgcctgtcca
    20881 tctgcattaa caacaatgcc gttttttatc aatacgcgca tagcaaactc caaaaaccct
    20941 ccggtgaacc ggaggggaat aagtgaagag taattattcg gttgccagcc agcttaatgg
    21001 gattgcagcg tacatagcgg cacaggttac caggtgagat ttccaggttt tttcgtttgg
    21061 cgcgtgcgct tccggttctt taccggggcc aaaaccgatg accggaatgc cgtggcgacc
    21121 catgatggat acgccattag tcgagaaggt ccacttatca acgacaggcg ctttaccaaa
    21181 cagcccttca taagcattca ccagtgcttt aacggtgaag tgatcttctt ccactttcca
    21241 ggtcgggaag tagcattcgg ttgggtaaac caggccagtc caggacgggc ggtcgtagtt
    21301 gtacatagaa acgacagcat tcgctttttg tactgcaggc agggcacgga tctcgtccag
    21361 cgcgccttcc caggtttcgc cccaggtcag acgacggtcg attgaaactg cgcagctgtc
    21421 tgcgacagcg caacggcttg gggaggtgaa gaaaatttca gaaacggtga gggtgccttt
    21481 gccgaggaat tcgtcataac ccagacgttg ggagagttct tgtaattcgc caagaatcgg
    21541 acccattttg aaaatggcgt tatcaccgcg ttctggtgcc gaaccgtggc agctaacacc
    21601 ctgaacatca atacgaattt ccatacgacc gcgttgacca cggtatacct ggcagtcggt
    21661 tggttcggta ctgaccacaa attccgggcg aatgccggat tgttcaataa tgtactgcca
    21721 gcacaggccg tcgcagtctt cttcctgcac agtaccggta accagcaggg tatattcatc
    21781 ttccagaccg agatctttaa tgattttacc ggcatatacc atagacgcca tgccgccttc
    21841 ctggtcggaa gtaccacgac caccgatcag ctcgtcggtt tccatgcctt catacggatc
    21901 gaagtcccag tttttgatgt tgccaatgcc gactgtatcg atatgagcgt ccattgccac
    21961 cagacgcggg ccgtggccga tataaccgag aacgttgccc attgggtcga tttcaacttt
    22021 atcgaagccg actttttcca tctcttcttt aatacgatgt actacgcgtt tctcgtcgca
    22081 gctttcactg ggaatagcaa ccatatcgcg caggaagcga gtcatgtctg cctggtaatc
    22141 ttttgctttt tcaagaatca gtttgaatgg aatattctta gccattattt ttctccagat
    22201 tttttactcc ccgttttgca gggagcagtt ccaatagggt gattaaggtg ctacagcgtg
    22261 tttgccttcc cagacaactt cgcggtagtg cttcacgtcg gtgtcgcctt cagtgctgat
    22321 aaccagcacc acggcatctt tgttcagcgc cagtttttcc atcaggcttt gacgttgcgg
    22381 gtgataatga accgctgcga gaacgcccaa accgacagcg ccggattcac cggagatgat
    22441 gcgcgggtcg ttgccgtacg gattacccag cacgcgcata cctaatgcgg caacgctgtc
    22501 ctggcaggag atgaattggg tggcgcagtt acgtaggatt tcccagccca gcgggttagg
    22561 ttcgccgcag gccaggcccg ccatgatggt ggccatatca ccgccaacgt tgacgatgtc
    22621 gcctttgacg ccggagcgat aaatacagtc agctttgtca ggttcaacaa taatgctgtg
    22681 cagattttgc gggctataga cgtcgaccag ataacccagc acaccaccgg ccattgcacc
    22741 gacacctgct tgcagcagaa cgtgtgtcgg ggttacgccc atttcacgca tttgctcgac
    22801 ggcttcatcc gccagtgttg cgtagccttg catgatccag gttgggattt tggtgtaacc
    22861 ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc tgcgcgtgtt gcatggtcag
    22921 gcgaacggta tcgtcatagt tcatatccgt gacgatgcac tcggcaccga ggtttagaat
    22981 ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa atcaccgcat tctgtccgag
    23041 ttgctgagcg gcccatgcca caccgcgccc gtggttgccg tcggtggtgg tcgcgaaagt
    23101 cattttttcg ccaatggcat ttttcaggtg ctcaaatgat agcgtttcga tatcaagatg
    23161 atatttttca cacaataatt gagcgatggc gtatgcgccg ccgagcattt tgaaggcgtt
    23221 cagaccgaag cgttttgatt cgtccttaac gagaattttc tttacaccaa acaggtttgc
    23281 gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca gcaattttct ggtggaactg
    23341 gcgtgccagt tttgcctggc tttgcgaaaa aagcggtgat gtttcgccgt tgaaaaattt
    23401 gttatcggcg atatcaatct ttaatgagaa aacggacata tcctatcctt ttggcctctg
    23461 gaaatagagg gccgaccagc tgatcggccc gaggggttat ttcacgcgtt cttgcgcttc
    23521 tttcagcagt tgctcgagca gtgcacctgg tttggcgtat ttacgggaca ggatcatcgc
    23581 ggcgatgata taaggcttcc agctggcttc tttgtaggta gcgatacggt atttttcgaa
    23641 tacgccttca gtcacttcac cttctttgca ggatacgccg ctgatatcag ctggcaggca
    23701 gtgcatgtac agggcttcgc catcacgggt cagctccatc atctcttcgg tacaatgcca
    23761 gtctttgtgc tgtgcgttct gtgccagaca ctgtttttcc agcgctttca gtccttcgtg
    23821 atcgttcgca cgcagcagtt cagtacgctc ttccatcact ttgtaaggtg cccaggactt
    23881 cggataaacg atgtctgcgt ctttgaaggc ttcttccatg ctggtgacct gacggaagct
    23941 accaccggag gctttagcat tgtttttagc aacttcaacg acatccggga tcaggtcgta
    24001 gccttccgga tgggccaggg tgacatccat accgaagcgg gtcatcagac cgatgatgcc
    24061 ttgtggtaca gagagcggtt tgccatagct tggggagtag gcccaggtca tggcgatttt
    24121 tttacctttc aggttttcca gtgaaccaaa gtgttcacgt aaccatgcca gatcagccat
    24181 tgattgagtc gggtggtcaa tatcgcattg caggttcact aaagccggac gctgcggcag
    24241 cacaccctgc ttgtaaccgt catcaagtgc agcgccaact tcacgcatat aggcgttgcc
    24301 tgcgcccaga tacatatcgt cgcgaatacc gatagcgtca gcgcagaagg agatcatatt
    24361 ggcggtttca cgcacggttt cgccgtgagc gatttgtgat ttaccttcgt caagatcttg
    24421 ttgtgcaagg ccgagcaggt taagcgcgga agcataagag aagcgggtac gggtggaatt
    24481 gtcgcggaat acggaaatac ctaatccact attaaagact ttggttgaga tgttttcagc
    24541 acgcagtgct tttaacgcgg cggcaacgtc cagtacttgt ttcagttcgt ctggcgtctg
    24601 ttcccacgtt aacaaaaaat ctttttcatg caggtgagag gtcagcgaat tgatatcctt
    24661 aatcagctca ttaacagttt tcatcataaa gtcccgatag tggaatgaca ggcaaagtgg
    24721 tttatttcac tgtgacgtgc caaagtgatt ggtgttttca cagctatacc tcggactaac
    24781 aataagtgtg ccagaattat ccacgcggct gcgaaaggct aatttgacgg ataatgtgtg
    24841 gggttgatca catattgatg ccagtttccg gttgtttgta aaatacgaaa acgtttggct
    24901 ttttttatta cgttatttat ttgctgctaa gtatcagaaa taagataaag aggcaggcta
    24961 tctctcttca gtttgataaa cttgccg
//
LOCUS       DAPQZD010000062        24164 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPQZD010000062 DAPQZD010000000
VERSION     DAPQZD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.062"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            221..2464
                     /gene="pdeF"
                     /locus_tag="RO454_002446"
     CDS             221..2464
                     /gene="pdeF"
                     /locus_tag="RO454_002446"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDY0934265.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2503..4044)
                     /gene="ppx"
                     /locus_tag="RO454_002447"
     CDS             complement(2503..4044)
                     /gene="ppx"
                     /locus_tag="RO454_002447"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDY0934266.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4049..6115)
                     /gene="ppk1"
                     /locus_tag="RO454_002448"
     CDS             complement(4049..6115)
                     /gene="ppk1"
                     /locus_tag="RO454_002448"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDY0934267.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6287..6925)
                     /gene="purN"
                     /locus_tag="RO454_002449"
     CDS             complement(6287..6925)
                     /gene="purN"
                     /locus_tag="RO454_002449"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDY0934268.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6925..7962)
                     /gene="purM"
                     /locus_tag="RO454_002450"
     CDS             complement(6925..7962)
                     /gene="purM"
                     /locus_tag="RO454_002450"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDY0934269.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8287..8913
                     /gene="upp"
                     /locus_tag="RO454_002451"
     CDS             8287..8913
                     /gene="upp"
                     /locus_tag="RO454_002451"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDY0934270.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8999..10288
                     /gene="uraA"
                     /locus_tag="RO454_002452"
     CDS             8999..10288
                     /gene="uraA"
                     /locus_tag="RO454_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDY0934271.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10338..11084
                     /gene="hda"
                     /locus_tag="RO454_002453"
     CDS             10338..11084
                     /gene="hda"
                     /locus_tag="RO454_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDY0934272.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11222..11581)
                     /gene="arsC"
                     /locus_tag="RO454_002454"
     CDS             complement(11222..11581)
                     /gene="arsC"
                     /locus_tag="RO454_002454"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDY0934273.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11602..13065)
                     /gene="bepA"
                     /locus_tag="RO454_002455"
     CDS             complement(11602..13065)
                     /gene="bepA"
                     /locus_tag="RO454_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDY0934274.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13278..14339
                     /locus_tag="RO454_002456"
     CDS             13278..14339
                     /locus_tag="RO454_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDY0934275.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14437..14907)
                     /gene="bcp"
                     /locus_tag="RO454_002457"
     CDS             complement(14437..14907)
                     /gene="bcp"
                     /locus_tag="RO454_002457"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDY0934276.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14907..15479)
                     /locus_tag="RO454_002458"
     CDS             complement(14907..15479)
                     /locus_tag="RO454_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDY0934277.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15625..16503
                     /gene="dapA"
                     /locus_tag="RO454_002459"
     CDS             15625..16503
                     /gene="dapA"
                     /locus_tag="RO454_002459"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDY0934278.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16520..17554
                     /gene="bamC"
                     /locus_tag="RO454_002460"
     CDS             16520..17554
                     /gene="bamC"
                     /locus_tag="RO454_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDY0934279.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17767..18480
                     /gene="purC"
                     /locus_tag="RO454_002461"
     CDS             17767..18480
                     /gene="purC"
                     /locus_tag="RO454_002461"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDY0934280.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18648..19511
                     /locus_tag="RO454_002462"
     CDS             18648..19511
                     /locus_tag="RO454_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDY0934281.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19526..21541
                     /gene="tmcA"
                     /locus_tag="RO454_002463"
     CDS             19526..21541
                     /gene="tmcA"
                     /locus_tag="RO454_002463"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDY0934282.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21615..22313
                     /gene="ypfH"
                     /locus_tag="RO454_002464"
     CDS             21615..22313
                     /gene="ypfH"
                     /locus_tag="RO454_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDY0934283.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22394..22594)
                     /locus_tag="RO454_002465"
     CDS             complement(22394..22594)
                     /locus_tag="RO454_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDY0934284.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22622..23749)
                     /gene="dapE"
                     /locus_tag="RO454_002466"
     CDS             complement(22622..23749)
                     /gene="dapE"
                     /locus_tag="RO454_002466"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDY0934285.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23753..24109)
                     /locus_tag="RO454_002467"
     CDS             complement(23753..24109)
                     /locus_tag="RO454_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDY0934286.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5635 a         6007 c         6354 g         6168 t
ORIGIN      
        1 ttaattattt acaatgatga tgtaataatg atgagttaac atgataaacg taataattca
       61 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
      121 cattgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
      181 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta
      241 tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc
      301 cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaagttt ttctctttta
      361 tctgccactg gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg
      421 gatcgcctta gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc
      481 attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca gggtttttac
      541 cccccggcgt aacaacgttt cacatggtga tagccgttta atttcccagc gactattctg
      601 gcagattgtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg cgtttgtagg
      661 gttactggcg agcagagaaa atctggtcgg cgtcatgccc tttaacctcg ggaccttaat
      721 caattatcag gccttgctgg tgggtaatct gattggtgtc ccgctgtgct actttatcat
      781 tcgggtggtg cgaaatccgt tttatttacg tagctattat tcgcaattaa aacagcaggt
      841 tgatgccaaa gttaccaaaa aagagttcgc aatctggcta ctggcattag gtgccttatt
      901 gttgctgtta tgcatgccgt taaatgaaaa aagcacgatt tttagcacca attacacctt
      961 gtcattattg ctgcccctga tgatgtgggg agcgatgcgc tatggttata agttgatttc
     1021 attgctctgg gcggtcgtgt tgatgatcag catccatagc tatcaaaatt acattcccat
     1081 ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt
     1141 tattgtcaat tatatggctg tactggcaac ccgtcagcga gctgtagtca gacgcattca
     1201 gcggcttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc
     1261 gttgcgtgat gccccctggt ctgcgctatg ctatttacac atccctggca tggaaatgct
     1321 ggttaagaac tatggcatca tgctgcggat tcaatacaag caaaaacttt ctcactggct
     1381 gtcaccattg ctggaaccgg gtgaagatgt ttatcagctt tcgggtaacg atctcgcgct
     1441 gcggctgaat acagaatcgc accaggagcg cattaccgca ctggatagcc atctcaagca
     1501 atttcgtttc ttttgggatg gaatgccgat gcaaccgcag attggcgtca gttactgcta
     1561 tgtgcgctcg ccagtgaatc atatctacct gctgctggga gagctaaata cggtggccga
     1621 actttccatc gtgaccaacg tcccggaaaa tatgcagcgt cgcggagcaa tgtatttgca
     1681 acgcgaattg aaagataaag tcgcgatgat gaatcgacta cagcaggcgc tggaacacaa
     1741 ccattttttc ctgatggccc agccgattac cggtatgcgt ggtgatgttt accatgaaat
     1801 tcttctgcgc atgaagggtg agaatgatga gctgatcagc cccgatagct tcttaccggt
     1861 cgcgcacgaa tttggtttat cgtcgagtat cgacatgtgg gtcattgagc atacgctgca
     1921 atttatggct gaaaacagag cgaagatgcc cgctcaccgt tttgctatta atctgtctcc
     1981 aacctcggta tgtcaggctc gttttcctgt tgaagtcagt cagctgctgg ctaaatatca
     2041 gattgaagcg tggcaactta tttttgaagt caccgaaagt aatgctctga ccaatgttaa
     2101 gcaggcgcaa atcaccttgc agcatcttca ggaattaggc tgccagattg cgattgatga
     2161 tttcggcacc ggctacgcca gctatgcgcg gcttaaaaat gtgaatgccg atctgcttaa
     2221 aattgacggc agttttatcc gcaatattgt gtcaaatagt ctggattatc agatagtggc
     2281 gtcgatttgc cacctggcgc gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa
     2341 cgaagagatc cgcgaggcgg tgctctcttt ggggatcgat tatatgcagg gttatcttat
     2401 tggtaagccg caaccgttaa ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc
     2461 ctgaataatg cgggccgaca tttctcgtcg gcccgcaaag cattaagcgg cgatttctgg
     2521 tgtactttct tcttcaattt tcaaccgcca gccagccacg ccttcccagt attcttgctc
     2581 cttttccaga tcaagcagta ccagcgcatt ctgactaaac cagtcatgcg ggaaacgcag
     2641 tgtccagtga ctgtcatcag taatcagcgt caatgttggc ggtgtggtgg ttgcctgacg
     2701 ttgattattg agtaatacgc caaggcgcaa tagctgaatc agtggcagga actgtttctt
     2761 cttaaacaag gtaaagcgcg gcagatcgtc gagtttaatc gctttacggt gatagcgcac
     2821 cagtgtcgcc atcatcagct gctgttcctg attaaaaccc ggcaagtcgc tgttttgcag
     2881 aatataagcg gagtggcgat gcaaaccgct gtggttgata ttcaacccga cctcatgcag
     2941 catggcggcc catcgcagta gcgcctccag ttgtggatgc gccagcttcg gttgctgttc
     3001 ccgccactgt tcgtacattt gcatagtggt atccagcacc cgtcgggcct gttcgctgtc
     3061 gatgtgatac tggttggcga ggctgctggc agtgcgacta cgcacatcct gatgacggaa
     3121 acgtccttcc atttcataca gtacgccttc gcgtaacgcc ccgtcagaaa ggcgcagttc
     3181 acggatggct aaagcatcaa acacaccgca taaaatcgcc agtcccggaa cgaagactgt
     3241 tttccgttct tcggaaagac ccggtaaact cagcgatgcg aaattacggt gacgtaaaac
     3301 ttcttttacc agtttttcca gacgttccgg ggtaattatc ccgtctttct cgcccatttc
     3361 cattaacact tcatgggcgg cttttatggt accggaagcg cccattgcca cgttccagcc
     3421 ctgaattcga aattgccagg ttaacgtttc cagtttttgt gctgccgcca tgcgagcgcg
     3481 ctgaaaattc tctttattga tgaccccgcc agggaaataa agctgggcaa agctgacaca
     3541 acccatccgg cggctttcaa cgagaatcgg ttcgaaattt tcaccaatca ccagttctgt
     3601 agaaccgccg ccaatatcaa taaccagttt gcgacctttt tccggttggg tatgttccac
     3661 gcccataaaa atcagacggg cttcttcatt accggaaata atttcgatcg ggtagggaat
     3721 gactttttcc gcgcgtttca gaaagtcagt ggcgttcagc gcctgacgca gcgtatgggt
     3781 accaactata cagacgctgg caggggaaaa cccttgtagc ctttcggcaa atagcgacag
     3841 acagttcaaa ccgcgcgtca ttgcctcttc actcaacata ttatctggcc ccaggccgtc
     3901 cgccagatgc acccgctgtt tcaggcggcc aataatctgc atggcaccat ctaccacacg
     3961 ggctatgacc atgtgaaaac tgtttgaacc aagatcgacc gcagcaaact cctgcggacg
     4021 aggggattta tcgtgtattg gcatagggtt attcagattg ttcgagtgat ttgatgtagt
     4081 cgtaaatcgc caactgcgcc cgtactttgc ggcgattgcc gcggggaacg tagcgattac
     4141 tgagttcttt atcgatataa cgtgctttga ccgtatcgct gaacaatatg tcgatgatgt
     4201 ccagcacccg ctgcttcagg cgcggatcga gcagcggcgt tgccacttca atacgataat
     4261 caatattgcg cgtcatccag tcggcggaag aaaggtagac ctttttatcg ccgccatttt
     4321 caaaaatata aacccggtca tgttcaaggt aacggtcaac aatactgatg gcacgaatgt
     4381 tgtcgctaat gccttccaga ttggggatca gagaacacat tccgcgcacc agcagattaa
     4441 ccggtacgcc ggagctggag gccgcataca gacgatcaac cagaccttta tcgacaaggt
     4501 tatttagctt cagggtgata ccactgggca gcccttgctg cgcgttggcg atctcgcggt
     4561 ccaccatttc atacaacaga cggcgagagt tttgcggcga caccattaaa taatcaaatg
     4621 tcaccggacg gtatgggttt tcaataaagt tgaacacccg ccgtacttcg ttagtgatgc
     4681 gcgcatcggc ggtcagcaac gaatagtcag tataaagacg cgcggttttt tcgttaaagt
     4741 tcccggtccc gatgtgtgcg taacgcacca cttcaccgtt ttctttacgt gaaatcagga
     4801 acagtttggc gtgaattttc agccccggcg cagagaagat aacgtgcacg cctgcttcgg
     4861 tcaggcgctt cgcccagtga atgttggctt cttcgtcgaa acgtgcctgt aactcaacca
     4921 ccacggtgac tttcttaccg ttgtgtgcgg cgtggatcat cgagtcgatg atgcgtgaat
     4981 ctttcgccac gcggtaaatg ttaattttaa tcgccagtac gctcgggtcg aacgaagcct
     5041 gacgcagcag ttccagtaca tgctcaaagg tatgataagg ataatagagc agcacatcgc
     5101 gctcacgaat ggcatcaaaa ccgttgcgga actgggcttt atcaaaccaa atgtggcgta
     5161 aacgtggcag tggtttgttc accagattgg ctttgccgac attggggaaa ttaataaagt
     5221 ctttaaaatt atgataacga ccgccgggga cgatggagtc gtagcgggaa atagtcagtt
     5281 tttcgcgtaa cacttcaacc agcgcattgg gcatatcgcg ctgataaaca aaacgcaccg
     5341 gctcagcagt taaacgctgc ttgagactgg aagacatcaa ctccatcagg ctggcttcca
     5401 tctcatgcac taaatcgtat tcggcatcgc gggtcatctt cattgaatag gcattcagcg
     5461 cgtcataatc aaagaagcct ttgaaaatat catcaaggca gtaacgcaga atgttatcca
     5521 gaagaatcat cggcttgcgt cgacgcggcg cttctggcgg taaattcaca aagcgcggca
     5581 ctttatctga tgggatctcc agcagcgcgt aacggatggt atcgccacgg ataatttcca
     5641 ccgccagata ggtgtaatca tctttcagga actgcactaa atcagtgtca ggattgatta
     5701 aaatcggcgt aatgtgctga cgcagatact gcttaaaata atgacgcagc cagttttgtt
     5761 gattgacgga gagctggcgt tcattaatca ggaagatctg gttgcgcgcc atctctagca
     5821 acaactcgtt gtagaggccg tcgaattcct gatcggcttt cagcacccgg gactgaattt
     5881 tgcccagtaa atggcgggaa tgagagttgg aaccttgttc ttcgctaata atgatgcgtc
     5941 gcttcagttc agcgaagcgg actttataga actcatcaag gttattggaa tagatcccca
     6001 ggaaacgcat cctttcaatc agcgggttag atttgtccgc cgcttcctga agcacgcgtt
     6061 cattgaacga taaccaactg agctcttttt cgatgtatag cttttcctga cccattacca
     6121 cttttactcc gttttattca cgggacactg cctggatatt atggcgagca atattggcag
     6181 atgtccaact atgccagaaa agggggaaaa aaactgctgg agcttgcgat ttacaggtga
     6241 attgaaaaac gccagcggca gagctggcgc tttaactacg ggggcattac tcgtcggcag
     6301 cgtagccctg cggcggcaga cgttgaccat ccagccacgc ggcgttttcg tgcattttca
     6361 gacgaccatc ggcaaaccag ctaatcacca gtggataaat agcgtgttcc tgggtttgca
     6421 cgcgggcggt gatgtcatct tccgtatcac cagcaaatac cgggactttc gcctgtaaaa
     6481 taaccgggcc accgtccagt tcatcggtga cgaaatgcac cgatgtaccg tgctcttcat
     6541 cgccattttc cagcgcctga cgatgggtgt gtaatccggg atatttcggc agcagagaag
     6601 ggtgaatgtt cagcaaacgc ccagcatagt gggagacaaa cgccgggctg agaatgcgca
     6661 taaaaccagc cagcacgacc acatcgggtg cgtacatgtc gatttcatga atcaactccc
     6721 ggtcataggc ttcacgactg tcaaacgcgc tggtgatgag cgtatgcgtt gcaatacccg
     6781 cctggcgggc gcgttcaagg ccgaacgcgt cggccttatt gctgaaaact gcccgtacgg
     6841 tgcctttaat tttgttggtt ttgcaggcgt caataattgc ctgcaaatta cttccgttgc
     6901 cggaaataag caccacaata ttcattattc gataaccacg cgttgttcgg aatcagaggc
     6961 tttgatgata ccgattttcc acgcgttttc accgttggca ttgagcaggg cgagggcttt
     7021 gtccacttcc ggagcaggca gggcaataat cataccgacg ccgcagttga aggtgcgata
     7081 catttcatgg cgctcaacgt tacctgccgt ttgcagccag ttgaacactt ccggccactg
     7141 ccaggaagat tcatcaatca ctgcctgggt attatctggc aatacgcgcg gaatgttttc
     7201 ccagaagccg ccgccggtca ggtgcgcaat ggcatgcaca tcgacctttt caatcaattt
     7261 cagcactgac ttcacgtaaa tgcgggtcgg tgccagcaga tgatcggcta atggcttacc
     7321 atcaagttcg gtggtttgcg gatcacaacc gctgacttca agaattttgc gcaccagcga
     7381 atagccgttc gagtgcgggc cgctggagcc gagtgcaatc agcacatcgc cgtcgctgac
     7441 tttagatccg tcgatgattt ctgatttttc taccacgcca acgcagaaac cagcgacatc
     7501 gtaatcttca ccgtgataca tccccggcat ttccgccgtt tcgccaccca ccagtgaaca
     7561 gcccgattgc agacaacctt ccgcaatgcc gctgatcacc gctgaagcgg tatcaacatc
     7621 cagttttccg gttgcgtaat agtcgaggaa aaacagcggt tccgcacctt gcaccaccag
     7681 gtcattaacg cacatggcga ccagatcaat accaatggtg tcgtgacgtt ttaagtccat
     7741 cgccagacgc agcttggtac ctacgccgtc agtgccggaa accagcacag gttcacgata
     7801 tttttgcggc aatgcacaca gcgcaccgaa gccgcccaga ccgcccatca cttccggacg
     7861 acgcgttttc ttcactacgc ctttgattct tccaaccaga gcattacccg cgtcaatatc
     7921 aacaccggca tctttgtagc taagagaggt tttatcggtc actgcttggg tccccacgcg
     7981 ttacttgcgg tagaaaaata aaattcggcg caattctaac agggaaagca aacgtttgcg
     8041 agactgcttt acacaacctt tttgcacgtc ttttccccag gcgcgcggcg aaagaatact
     8101 tgtaccaggg caaaggatcg ttttcggatg gaataatctt ctttcataac tatttgaata
     8161 taaaataact ttatctcaaa ccgttatcat tttgactaaa gtcaacgaaa ataatattgc
     8221 cgccttgaag aaaggaggta taatccgtcg attttttttg tggctgcccc tcaaaggaga
     8281 aagagtatga agatcgtgga agtcaaacac ccactcgtca aacacaagct gggactgatg
     8341 cgtgagcaag atatcagcac caagcgcttt cgcgaactcg cttccgaagt gggtagcctg
     8401 ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag taactatcga aggctggaac
     8461 ggcccggtag aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt
     8521 gcgggtctgg ggatgatgga cggtgtgctg gaaaacgttc cgagcgcgcg catcagcgtc
     8581 gtcggtatgt accgtaatga agaaacgctg gagccggtac cgtacttcca gaaactggtt
     8641 tctaacatcg atgagcgtat ggcgctgatc gttgacccaa tgctggcaac cggtggttcc
     8701 gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg
     8761 ctggtagctg caccagaagg tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg
     8821 tatactgcat cgattgacca gggactgaac gagcacggat acattattcc gggcctcggc
     8881 gatgccggtg acaaaatctt tggtacgaaa taaagaataa aaataattaa agccgacttt
     8941 aagagtcggc ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat
     9001 gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag
     9061 tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat
     9121 taacccggcg actgtactgt tatttaacgg tataggaaca ctgctgtatc tcttcatctg
     9181 taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac cggtattgtt
     9241 actgttgccg ttggggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt
     9301 ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc
     9361 acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc
     9421 cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat
     9481 ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc
     9541 aattatcccg attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat
     9601 tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccgaccc tctatacgcc
     9661 gcgcttcgag tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga
     9721 acacgtaggg cacctggtag taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc
     9781 aggtctgcac cgttcgatgt ttgctaacgg cttgtcgacc gtgatctccg gcttctttgg
     9841 ctccacgcca aacaccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta
     9901 cagtacctgg gttatcggcg gtgcggcgat tttcgctatc ctgctttcct gcgtcggtaa
     9961 actggctgct gctatccaga tgattccatt gccggtgatg ggcggcgttt cgctgctgct
    10021 ttacggtgtc atcggtgctt ccggtattcg tgtgttgatc gaatcgaaag tggattacaa
    10081 caaagcacag aacctgatcc tgacttccgt gattttgatc atcggcgtca gtggcgcgaa
    10141 ggtaaacatt ggggcggcgg agctgaaagg tatggcgctg gcgaccatcg tcggtatcgg
    10201 cttaagtttg atcttcaaac tgattagtat gctgcgccct gaagaagtgg tgctggacgc
    10261 agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc
    10321 aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat
    10381 ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg
    10441 caagtttctg gccgggggat aactcctctt tactggccgc gctgcaaaac gtgctgcgtc
    10501 aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc
    10561 tgcacgcggc ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat gtcccgctgg
    10621 ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct
    10681 gtattgacaa cattgagtgt attgccggcg atgagttgtg ggagatggcg attttcgatc
    10741 tctacaatcg aattctggaa tcgggcaaaa cacggttgtt gatcaccggc gatcgtccac
    10801 cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg tctcgactgg gggcagatct
    10861 acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt
    10921 tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag
    10981 aaatgcgcac gttgtttatg acgctggatc agctggatcg tgcgtcgatt accgcgcaac
    11041 gcaagctgac cattcctttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt
    11101 cggatgcggc gcgaacgcct tatccgacct acgcgaagtt aatccatagt attcgtaggc
    11161 cggataagac gcggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat
    11221 cttaacccac gatttccagt acctgctccg gtggacgacc aatccgtgct ttgccattcg
    11281 cgaccacaat cgggcgttcc attagtttcg gattatccac catcgcctga atcagcgcct
    11341 cttcgctaag tgaactgtca gccaggttca gttctttata aagatcctct ttttggcgca
    11401 tcaattcacg agcgctattc atccccagca ttttcagcaa atcacgcagc gttgccgcat
    11461 cggcgggtgt ctcaaggtag agcaccactt ctggctccac gccgttttct ttcagcaggt
    11521 tcagcgtttc ccggctcttt gaacagcgtg ggttatggta gatttttacc tgtttggtca
    11581 ttgttcttcc tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg
    11641 gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg
    11701 cgaactggcg ctactcaaca aggaaatagc ctgatcgagt cgcccggcga gcgcataacc
    11761 ttctgctcgc gcagccagtt cctgatcgcg gttatttagt gcggcttccg cctgtgccag
    11821 taaatcccag ccgttgctgt catctttatt gttaaaagtg tagcgattca gaatattggc
    11881 cgcttcctgt ggttgccccc cttgcagata agcgttcgcc aggttgagct gcaacaccgg
    11941 attggtgcgc aaatcgcggg catttttcag gcgattgata gcgtcattgg ctttgttttg
    12001 cccaagatcg atatcggtag ccagatcgag ataccatgcg ttgccaggtt ctgctgccag
    12061 taacggttgc agcgttttac gcgcttcgtc gtatttattg gcttccatcg cctgtagagc
    12121 acgaccatat tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa
    12181 atcactggtg agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag
    12241 atagaaatct tccgacgact gcaccaccat cgggcgcatc tgattagcac ggttgcgggc
    12301 atctgccaga cgactttccg gcaacgggtg agtcaataaa atttccggcg gacgcgagga
    12361 gtaacgcgcc tgatcgagta atttttcgag gaaggtcggc atcgcctgcg gatcgaatcc
    12421 cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa
    12481 actgatcatc ccctggcgcg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg
    12541 cggactggcc atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct
    12601 gcgctgctga tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg
    12661 cgccataact gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag
    12721 caccacgttg ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaagaa
    12781 atgaaacggt gtcttaaccg aattggcatg cgaaaccaga cgcattccta gcgaattaat
    12841 atattgcgtt aatagcgggt cattaattaa cggcgcgctg ccgcgtaact ggcggacata
    12901 atagtcgccc atctgcattt cctgaccaat ggaaagtgtg cttcctgcgg aggttcccat
    12961 atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc
    13021 agcaatgagg gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta
    13081 tttctggaga tagtcatttg accgatactg tgaaatatcg ttcccaccgc gccgcctttg
    13141 agtgtaacga cgaagattag cccactcaaa taacttttca gtttcctgaa tccgtcataa
    13201 aaagcgaagt cttttttgtg acatttcgat acaattgcgg atcgcaatcc cgctcttgag
    13261 gttcaggtaa ggtctgtatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg
    13321 acccggaagc gattgccttg ctggttattt tagtcgccgg atttggcatt atctttttct
    13381 ttagtggcct gcttgctccg ttgctggtgg ctattgtgct ggcctatttg ctggagtggc
    13441 caaccgtgcg cctgcaatct attggctgtt cccgccgctg ggcgacgtcg attgtattgg
    13501 tggttttcgt cggtatattg ctactgatgg cgttcgtggt tctgcctatt gcctggcagc
    13561 agggcatcta cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg
    13621 ccacgttgcc gcgccgctat ccggcgttaa tggatgcggg cattattgat gcaatggccg
    13681 aaaatatgcg cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct
    13741 cgttggtcgg tttgctgacc attgccgtct atctggtgct ggtgccattg atggtcttct
    13801 tcctgctgaa agacaaagag cagatgctga acgccgttcg ccgggtgctg ccgcgcaacc
    13861 gtggactggc aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg
    13921 gcaaagtgct ggagatgatc gtggtaggga tcgccacctg gctggggttc ttgctctttg
    13981 ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt attccgtaca
    14041 ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggtgctg
    14101 gcacggaatt ctggagctgc ttcgcggtgt atctgattat tcaggcgctg gacggcaacc
    14161 tgttagtacc ggtgttgttc tccgaagcgg ttaacctgca tcctctggtg attattttat
    14221 cggtggtgat cttcggtggt ttgtggggat tctggggcgt attcttcgct attccattgg
    14281 cgacgctgat caaagccgtg attcacgcct ggcctgatgg gcaaatcgcg caagactgac
    14341 cttcagcgaa aaatttgaaa cgagtgttgc agggcagtga gaagatttga gatgtgctgg
    14401 ccgcaagcgc agccagcacg gaatggagca aagtaatcag gcgtgttctt tcagccagtt
    14461 cagcacaacg tcgtggtgat tgctggtttt gaaatcgtca aagacatgtt cgattttgcc
    14521 atcagcgtca atcaggaagc tgatgcgatg aataccatcg taggttttgc ccatgaagga
    14581 cttttcaccc cagacgccga attgttcgca cacctggtgg tcctcatcag acaggagcgt
    14641 aaagttaagc agctcttttt ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat
    14701 acccagcaca tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta agccgcaggc
    14761 ctgtacggta cagccggggg tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc
    14821 ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc
    14881 gatatcaccg gctttcagtg gattcattac ttaactccat cctgttcatc atgttgggaa
    14941 taattgacga cgttaatact gccttgtgca ttgagttctg tacatagggc tttgaacgct
    15001 tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg cagttatctg aatatgtaac
    15061 tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca ccagctccgc aatgttcata
    15121 tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg
    15181 acctgaaccc agacagatgc tggcattggt ggacgcggac gcgccgtcgt gcgcttcatc
    15241 acgattaaaa gatccagttc ggcacctttc aacggtaacg ttgattcaat cagggtgatt
    15301 gcattccatg aaccggaaag cagcatgata aacgtgaact cttctcccag catcgccagg
    15361 cgactgtctt caatattaca gccgcaacta ctgacatgac gggtgatggt gttcactatt
    15421 ccagggcgat cggcacccaa cgcagtgatc accagataat gttgcgatga cagtgtcaaa
    15481 ctggttattc cttcaagggg tgagttgttc ttaaggaaag cataaaaaaa acatgcatac
    15541 aacaatcaga acggttctgt ctgcttgctt ttaatgccat accaaacgta ccattgagac
    15601 acttgtttgc acagaggatg gcccatgttc acgggaagta ttgtcgcgat tgttactccg
    15661 atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa aactgattga ttatcatgtc
    15721 gccagcggta cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc taccttaaat
    15781 catgacgaac atgctgatgt ggtgatgatg acgctggagc tggctgacgg gcgcattccg
    15841 gtgattgccg ggaccggtgc taacgctact gcggaagcca ttagcctgac gcagcgcttc
    15901 aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa
    15961 gaaggtttgt atcagcattt caaagccatc gctgaacata ctgacctgcc gcaaattctg
    16021 tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg
    16081 aaagtaaaaa atattatcgg aatcaaagag gcaacaggga acttaacgcg tgtaaaccag
    16141 atcaaagagc tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg
    16201 gacttcatgc aattaggcgg tcatggggtt atttccgtta cggctaacgt cgcagcgcgt
    16261 gatatggccc agatgtgcaa actggcagca gaagggcatt ttgccgaggc acgcgttatt
    16321 aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg aacccaatcc aatcccggtg
    16381 aaatgggcat gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca
    16441 ccaatcaccg acagtggtcg tgagacggtc agagcggcgc ttaagcatgc cggtttgctg
    16501 taaagtttag ggagatttga tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc
    16561 gggtgtttcg cttgttttat tactcgctgc ctgtagttct gactcacgct ataagcgtca
    16621 ggtcagtggt gatgaagcct acctggaagc ggcaccgctt gcggagcttc atgccccggc
    16681 tggaatgatt ttgccggtga cctccggcga ttatgcaatc ccggtgacca acggtagtgg
    16741 tgctgtcggt aaggcgctgg acattcgtcc accagcccag ccgttggcac tggtttctgg
    16801 cgcgcgtacc cagttcacgg gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa
    16861 tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca tcacccaacg
    16921 tgatgatgct ggtcagacac tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga
    16981 cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg cagggttatc agcaggcggt
    17041 tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg cggcgtccat
    17101 gcagcgttac agcacggaga tgatgaacgt tatttccgcc ggtctggata aatctgccac
    17161 tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact atggacgtac aaagtgcagc
    17221 tgatgacacc ggtttaccaa tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg
    17281 tctgccagct gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg
    17341 caacatggcc gtaacttata agccgctgtc tgacagcgaa tggcacgaac tgggcgcgag
    17401 cgatccaggt ctggcatccg gtgactataa actgcaggtt ggcgatttag ataaccgcag
    17461 cagcctacag ttcatcgatc cgaaaggtca tactctgact cagagtcaga acgacgcgct
    17521 ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac agggctggag tcatccggcc
    17581 cttttttctg atatgatacg caaacgtgtg cgtcggcaga aaaacgcgat tttagcggta
    17641 attcgcacga aatttgtttg tcggacgtag gtcggataag gcgttcacgc cgcatccgac
    17701 aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg
    17761 ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg
    17821 gaaaacccgg acctgttggt gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg
    17881 cgcattgagc agtttgatcg caaaggtatg gtgaacaaca agttcaacta cttcattatg
    17941 agcaaactgg ctgaagcggg tatcccgact caaatggagc gtctgctctc cgataccgaa
    18001 tgtctggtga aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct
    18061 ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc gccgctgttc
    18121 gatctgttcc tgaaaaacga cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa
    18181 acctttggct gggtgagcaa agagaacctg gcgcgtatga aagagctgac ctacaaagcg
    18241 aacgacgtgc tgaaaaaact gttcgatgat gctggtctga ttctggtcga cttcaagctg
    18301 gagtttggtc tgtacaaagg cgaagtggtt ctgggcgatg agttctcccc ggacggcagc
    18361 cgcctgtggg acaaagaaac gctggagaaa atggacaaag accgtttccg ccagagcctc
    18421 ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc tgggtgtaca gctggactga
    18481 tttttctgtt catcatcttg ccgtgctgct ggcacggcaa gacaaccgct ctcataagat
    18541 gagtattgaa cgctcattcc tccgccattt catcccgctt tcctgtggta atcttcctct
    18601 gaaccaccta cgatcatttc tataatgaat ataatgttga ggtatctatg cgttggcaag
    18661 ggcgacgtga aagtgacaat gttgaagaca ggcgcaatag ctccggcggt ccgtcaatgg
    18721 gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct gattttactg atagtcgtgc
    18781 tggttgcagg ctactatggc gttgatttaa ccgggttgat gaccggacag ccggtttccc
    18841 aacagcaatc aacgcggtca attagcccta atgaagacga agccgcaaaa ttcacctcgg
    18901 tgattctggc aaccacggaa gacacctggg gacagcaatt cgagaagatg ggtaaaacct
    18961 atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg taccggctgc ggggcgggcc
    19021 agtccataat ggggccattc tattgcccgg cggatggcac agtttatatc gatctctcct
    19081 tctatgatga catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta
    19141 tcgctcatga agtcggtcat catgtgcaga aactgttagg catcgagccg aaagttcgtc
    19201 aactgcaaca aaacgcgacg caggcggaag taaaccgctt atctgtgcgt atggaactcc
    19261 aggccgactg ttttgccggt gtctgggggc atagtatgca gcagcaaggt gttctggaaa
    19321 ccggcgatct ggaagaggcg ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac
    19381 agcaaagtca ggggcgagta gtaccagaca gtttcactca tggcacttct cagcaacgct
    19441 acagctggtt taaacgtggt ttcgacagcg gcgatccggc acaatgcaat acttttggta
    19501 aaagcattta acttccgggg cagggatggc tgaactgact gcgcttcaca cattaacagc
    19561 gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg agcggggaag agcgttggtg
    19621 ttttgatcat gcgcttaagt tgcgtgatgc cttacctggc gactggctgt ggatttcgcc
    19681 gcagccagat gctgaaaacc actgttctcc ctcggcacta caaactttac ttgggcgcga
    19741 gttccggcat gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgcggcact
    19801 tagcggaacg ttgaaagcgg gaagctggct ggtgttgtta ctccctgtat gggatgagtg
    19861 ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat tgccctgacc ctattgcgac
    19921 gccgcatttt gtccagcatt tcaaacgcgt acttacggcg aataacgacg ctatcctctg
    19981 gcggcaaaac cagccgttct cgttggcgca ttttactccc cgtactgact ggcaccccgc
    20041 taccggcgca ccacaaccag aacaacagca actcttacag cagctactga ccatgccgcc
    20101 gggtgtggca gcggtaactg ctgcgcgtgg gcgcggtaag tcggcgttgg cagggcaact
    20161 catttctcgt attgcgggca gtgcgattgt caccgctccc gcaaaagcgg caacggatgt
    20221 actggcacaa tttgcgggcg agaagtttcg ctttattgcg ccggatgcct tgttagccag
    20281 cgatgagcaa gccgactggc tggtggtcga tgaagccgca gctatacctg cgccgttgtt
    20341 gtatcaactg gtatcgcgtt ttcctcgaac gttgttaact actacggtgc agggctacga
    20401 aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt
    20461 tgaactacaa cagccgatcc gttgggcgca gggatgcccg ctggaaaaaa tggttagtga
    20521 ggcactggtg tttgacgatg aaaacttcac ccatacacca caaggcaata tcgttatttc
    20581 cgcatttgaa cagacgttat ggcgaagcga gccagaaacg ccgttaaagg tttatcagtt
    20641 attgtctggt gcgcactacc ggacctcgcc gctggattta cgccgcatga tggatgcacc
    20701 aggtcaacat tttttacagg cggctggcgg aaacgagatt gccggagcgc tgtggctggt
    20761 ggatgagggc ggattatctc aagaactcag tcaggcggta tgggcgggtt ttcgtcgccc
    20821 gcggggtaat ctggtggccc agtcgctggc ggcgcacggc agcaatccac tggcggcgac
    20881 attgcgcgga cggcgggtta gccggatagc agttcatccg actcgtcagc gggaaggcac
    20941 agggcgacag cttattgctg gtgctttgca atatattcat gacctcgact atctttcggt
    21001 gagttttggt tacaccgagg agttatggcg tttctggcaa cgctgcggtt ttgtgctggt
    21061 gcgaatgggt aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc
    21121 gatgagtgat gcgggtaaac agctggctga acgtgagcat taccgtttac gtcgcgatgc
    21181 gcaagctctc gcgcagtgga atggcgaaat gcttcctgtt gatccactaa acgatgccgt
    21241 cctttctgac gacgactggc ttgaactggc cggttttgct ttcactcatc gtccgctatt
    21301 aacgtcgtta ggttgcttat tgcgtctgct acaaaccagc gaactggcat taccggcgct
    21361 gcgtgggcgt ttacagaaaa acgccagtga cgcgcagtta tgtaccacac ttaaactttc
    21421 aggccgtaag ctgttactgg ttcgccagcg ggaagaggcc gcgcaggcgc tattcgcact
    21481 tgatgatgtt cgcactgagc gtctgcgcga tcgcataacg caatggcaat tttttcactg
    21541 actccttcaa ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag
    21601 ttaaggagtt cgccatgaaa catgaccatt ttgttgttca aagcccggat aaaccagcac
    21661 aacagttgtt actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag
    21721 gtagctggtt tgcgccgctg tttcctgatg cgctggtggt gagtgtcggt ggcgcggagc
    21781 cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca gggtattacg gaagataatc
    21841 gccaggcgcg tgtgaatgcc atcatgccga cgtttattga gacggtgcgc tactggcaga
    21901 aacaaagcgg agtaggggct aacgccacgg cgctaatcgg tttctcacaa ggcgcgatta
    21961 tggcgctgga gagcattaaa gccgaaccgg gccttgcttc gcgcgtcatt gccttcaacg
    22021 gacgctacgc gagcctgccg gaaaccgcgt ctacagccac aacaattcac ttgattcatg
    22081 gcggtgaaga tccggttatc gatctggcgc atgcggttgc ggcgcaggaa gcgttaatca
    22141 gtgccggtgg tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc
    22201 gcagtatgca atttgccctg gatcacttgc gttataccat tccgaaacat tatttcgacg
    22261 aagcgctaag cggcggtaag cctggcgatg atgatgtgat tgaaatgatg taataatagc
    22321 cgggtaggtc tgataagact gtatcaggca tcatggccgg atgcggcgtt aacgccttat
    22381 ccggccactt agcttacttc ttcggttgat cctttttcgg ccagtcgtcg tcatcgtccc
    22441 acttatcgtt gaaatcacga tgcgggggaa gctccggctt gttggcgaga aatttcttat
    22501 ggtcgacgcg cttgagatct ttaatcacgt tcagcaggac gcctaccaaa aagacaatca
    22561 ccagaatcca ccaatattta gccagccagt ccatgcttat ttcctcttgc agaaccactc
    22621 atcaggcgac gagctgttcc atgatacgtt gatacatacg ggcaagtagc tgcaggtcgg
    22681 cagcgttcac acattcattg attttatgaa tcgtggcatt gaccggcccg agttccacca
    22741 cctgcgcccc catgcgggca ataaagcgcc cgtcggacgt tccgcctgtg gtcagtagct
    22801 gcggtttaat ttcattatag tgctcaaccg cgttaacgac cgcatccacc agtttaccgc
    22861 gcgcggtcaa aaatggctgc ccggaaagcc accaatccac cgtatagcgc agttgatgtt
    22921 tttcaagcag ggcaagcacc tgcgctttga tcatctcatc cgtcagttcg gtgctaaagc
    22981 ggaagttaaa ctgcacaaac agttcacctg gaataacgtt gttactgccc gttcccgcct
    23041 gaatattggc aatctgcata ctggtcgccg ggaagaagtc attaccctga tcccactcaa
    23101 tagccactaa ttcattaatg aaaggtgctg cgcgatgtac cggattgtca gccagatgtg
    23161 ggtaggcaac atgcccctga acgccatgaa tggtaaggtt gcaggttaac gatccgcgac
    23221 gaccattttt caccacatca cctaccactt cgatactcga cggttcgcca accagacagt
    23281 aatcgagacg ctcattacgc gccattaacg cttcgacgac ttttaccgta ccgttgtggg
    23341 cactagcttc ttcatcagag gtgatcagaa atgccagtcg ccctgtatgg ttgggatgtt
    23401 gtgcgacaaa gcgttctgcc gctaccacca tcgccgccag cgagcctttc atatctgccg
    23461 cacctcgccc aaataacatg ccgtcacgaa tggttggttc aaatggtgga ttgatccaac
    23521 ggtcggcgtc gccaggcggc accacgtcgg tatgcccggc aaaggctaac gtttcaccct
    23581 gcccgcgcca tgcccaaaaa ttctgcgtat cggcaaagtc catgcgttca acggtaaaac
    23641 cgatcgcctg caaacgttca atcaacaaag cctggcatcc tgcgtcatca ggactcaggg
    23701 aagggcggcg aataagctgt tgtgtcagct caataaccgg gcacgacata gactacacct
    23761 catggaaaaa ttgctgataa ctggattcac tgaaacccag cagcataggc ttaccaggcg
    23821 cgcagagcaa tggacgtttg ataattgcag gcatttcagt cattaatgtc gccgcagagg
    23881 ccgcatcggt gattttattg cgggtggttt cgtccagttt acgccaggtt gtaccacggg
    23941 tgttgagtaa cgcttcccag cctaattcgt tgataaaacc gttcaataat tcgctgtcca
    24001 atccatcgac gcggtaatca tgaaaacgat agtcgatgtt attggcttcc agccaacggc
    24061 gagccttttt aatggtgtca caatttttga tgccgtaaag tgtaaccata atgaatcctt
    24121 tttcgattta tgagaataaa tactcattta agggtgaaaa catt
//
LOCUS       DAPQZD010000063        24076 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPQZD010000063 DAPQZD010000000
VERSION     DAPQZD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.063"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            198..1511
                     /gene="gltP"
                     /locus_tag="RO454_002468"
     CDS             198..1511
                     /gene="gltP"
                     /locus_tag="RO454_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDY0934287.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
     gene            complement(2000..2689)
                     /locus_tag="RO454_002469"
     CDS             complement(2000..2689)
                     /locus_tag="RO454_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDY0934288.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2800..3504)
                     /locus_tag="RO454_002470"
     CDS             complement(2800..3504)
                     /locus_tag="RO454_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0934289.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(3491..4327)
                     /locus_tag="RO454_002471"
     CDS             complement(3491..4327)
                     /locus_tag="RO454_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0934290.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(4320..5153)
                     /locus_tag="RO454_002472"
     CDS             complement(4320..5153)
                     /locus_tag="RO454_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0934291.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(5153..6202)
                     /locus_tag="RO454_002473"
     CDS             complement(5153..6202)
                     /locus_tag="RO454_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0934292.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(6302..7870)
                     /locus_tag="RO454_002474"
     CDS             complement(6302..7870)
                     /locus_tag="RO454_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0934293.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(8245..10392)
                     /locus_tag="RO454_002475"
     CDS             complement(8245..10392)
                     /locus_tag="RO454_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9973..9975),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDY0934294.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(10590..12056)
                     /gene="mdtP"
                     /locus_tag="RO454_002476"
     CDS             complement(10590..12056)
                     /gene="mdtP"
                     /locus_tag="RO454_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDY0934295.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLNGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAISAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(12053..14104)
                     /gene="mdtO"
                     /locus_tag="RO454_002477"
     CDS             complement(12053..14104)
                     /gene="mdtO"
                     /locus_tag="RO454_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDY0934296.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            complement(14104..15135)
                     /gene="mdtN"
                     /locus_tag="RO454_002478"
     CDS             complement(14104..15135)
                     /gene="mdtN"
                     /locus_tag="RO454_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDY0934297.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVSDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(15154..15429)
                     /locus_tag="RO454_002479"
     CDS             complement(15154..15429)
                     /locus_tag="RO454_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934298.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(15638..17623)
                     /gene="yjcS"
                     /locus_tag="RO454_002480"
     CDS             complement(15638..17623)
                     /gene="yjcS"
                     /locus_tag="RO454_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDY0934299.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(17896..18825)
                     /gene="alsK"
                     /locus_tag="RO454_002481"
     CDS             complement(17896..18825)
                     /gene="alsK"
                     /locus_tag="RO454_002481"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDY0934300.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(18809..19504)
                     /gene="alsE"
                     /locus_tag="RO454_002482"
     CDS             complement(18809..19504)
                     /gene="alsE"
                     /locus_tag="RO454_002482"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDY0934301.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     RERKDLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(19515..20495)
                     /gene="alsC"
                     /locus_tag="RO454_002483"
     CDS             complement(19515..20495)
                     /gene="alsC"
                     /locus_tag="RO454_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDY0934302.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(20474..22006)
                     /gene="alsA"
                     /locus_tag="RO454_002484"
     CDS             complement(20474..22006)
                     /gene="alsA"
                     /locus_tag="RO454_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDY0934303.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(22134..23069)
                     /gene="alsB"
                     /locus_tag="RO454_002485"
     CDS             complement(22134..23069)
                     /gene="alsB"
                     /locus_tag="RO454_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDY0934304.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATETFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(23128..24018)
                     /gene="alsR"
                     /locus_tag="RO454_002486"
                     /gene_synonym="rpiR"
     CDS             complement(23128..24018)
                     /gene="alsR"
                     /locus_tag="RO454_002486"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDY0934305.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         5718 a         6232 c         6167 g         5959 t
ORIGIN      
        1 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
       61 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
      121 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
      181 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
      241 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
      301 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
      361 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
      421 aacagctcgg gcgtattggt gcgaaaacca ttatctactt cgaggtgatc accaccgtcg
      481 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
      541 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
      601 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatc gtggcgtcga
      661 tggcgaaagg cgaaatgctg ccgatcatct tcttctcggt gctgtttggt ctggggcttt
      721 catccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt tttccgctcc atctctgaaa
      781 ccatgtttaa agtgactcac atggtgatgc gttatgcgcc ggtgggtgtg tttgcgctga
      841 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
      901 tgctggtgca tttcgccatt ctgttcttcg cgctggtcgt actgggaatt gtggcgcgcc
      961 tgtgcgggtt aagtgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
     1021 actctactgc cagttctgaa agcgtgctgc cgcgaattat tgagaagatg gaagcctacg
     1081 gtgcaccggc gtcgatcacc agtttcgtgg tgccgaccgg ttactctttt aaccttgatg
     1141 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
     1201 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
     1261 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
     1321 cgctggaagg tctggcgttt attgccggtg ttgaccgtat cctcgatatg gcgcgtactg
     1381 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
     1441 ttgaccgtaa aaaagcgctg gcctacgagc gtgaaatgtt gggcaaattt gataaaacag
     1501 cagatcagta attgaagatt gccgggggga tatccacccg gcaatgtgtg gatgcctgac
     1561 tgagtaatag cctgatgtag cgtgattttt gtaggtcgga taaggcgttt acgccgcatc
     1621 cgacatcaac gcctgatgcg acgcttaacg cgtcttatca ggcctacgcc agacagcgca
     1681 atagcctgat ttggcgtgat ttttgtaggt cggataaggc gtttacgccg catccgacat
     1741 caacgcctga tgcgacgctt gccgcgtctt atcaggccta cgccagacag cgcaatagcc
     1801 tgatttggcg tgatttttgt aggtcggata aggcgtttac gccgcatccg gcatcaatgt
     1861 ctgatgcgat gctgacgtgt cttatcaggc ctacgtcaga cagcgcaata gcctgatttg
     1921 gcgtgatttt tgtaggtcgg ataaggcgtt tacgccgcat ccgacatcaa tgcctgatgc
     1981 gacgtttgct gcgcctacct taaccgttag ttaatttttc aaactcttca caaccggtat
     2041 caaatccttc catacagctc agattcaacc agtgcagcgc cttttgttta ttcttctcga
     2101 taaaaccctc ttcaccgttt aagaacatca ttcccgccca gtattcggaa taaccggttc
     2161 gggaaatcgc agagctacgt ttgaaatacc aggttgcttt gtcatcgtcg gccttaatgc
     2221 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca gagtcgttct
     2281 ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg
     2341 cttgcgtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
     2401 ctgctttctc ggcaagcact tttgcttgtg gatagtccag actgaccgga ttggtgattt
     2461 tgatctgcgc caacagcgcg caggcatcgg catcaccgct gtcggcggct ttctgtgccc
     2521 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
     2581 cgcgtcggtc tccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg
     2641 cgtgggcaaa gaatgtcaaa aacaacatca aagtgataat ttttttcatt ttgataatga
     2701 agtttggtag gggagagaca gtataaagtg ttcgggtgga caaaaaaaca actgtaaaaa
     2761 gacctgaaag cagaatgaat taatgcaatg tcaggttttt taatccatcc gatgttcccc
     2821 cttttccatg gcgtcgcgat caaaaaatcg ctgaattttt ccctcggaca tcaatgccgc
     2881 tcgatcagac atatgcgcga tgacatcggc atcgtgactg accagcagat atgtcatttg
     2941 gtgttccagc ttaagttgat tcaacaaatt aaggatttcc gcttgtaccg acatatccaa
     3001 cgccgaggtg ggttcatcca gcagaagaag ttgtggtcgt aacaacaggg cgcgagcaat
     3061 aaccacacgt tgccgttgac caccggaaag ctggtgtggg tagcgacgag cggcgtcgaa
     3121 aggcaagcca acctgttcca gtgcagtctg gacgcgtttt tctatttctc tttcgccacg
     3181 aattttgagt ggttcagcca gcgttcgcca cagagtatga ttgggatgca acgaggcata
     3241 gggatcctga aacaccattt gcacattgcg gcggagatcg ccctgaaaac gcattcctgg
     3301 tttgatggtg tgcccaaaca gtgacacctg accgtgccag tcgcgctgta atccagcaat
     3361 tacgcgcaaa atcgtggatt ttccgcagcc tgattcgcca attaaactaa atgtctctcc
     3421 ggcattaacc gaaaaactgg cggcagaaac tgcggttttc tcagcaaaag tcacctgtaa
     3481 gtgattaatt tcaacaatcg tcatggtatt tctccggcgt cattgccgtc ctgtccagcg
     3541 tcggcagcat ctgcccgtaa gtttgtgcat ttgggcggca agtccacagt gtgcgcgtgt
     3601 aaggatgcgt tgctgtaggt agcgcagcag cgtgcatttc atccacctta tttccctgat
     3661 acatcaccag cacccgatgg caaaattggg caactaatgg caggtcgtga ctgatcagca
     3721 acattgccat ctgacgttgt tcacactggg taaccagcag ttccagaatc tgattacgca
     3781 ggcgcacatc tagtgctgat gttggctcat cggcaatcaa tacccgggga tcgttgatga
     3841 gcgcaattgc aatcatcact cgctgcccca tgccgccaga aagttcacga ggatagcttt
     3901 gcagtactcg cgtattgagg ccgacagcct gaattgcggt gtttattttc tcttctttct
     3961 cgcggcggtt cagacgttga tggagcgtaa gcgcctcttc cagttgcgct tttactgtct
     4021 ttactggatt aagcgcgtaa cgcggatctt gcaataccat cgcgatatcg ttaccgcgca
     4081 actgttgcca tccacgcgcg ttaagggtga gtgcatcacg ccccagaatg ttaagtgtgt
     4141 ccgcggttac tacacccggt ttacgcacca gccccatcag ggcgcgggcg gtcatggact
     4201 tacctgaacc cgactcaccg accagcgcca gacgttcatt gcctaatgta aaactgacat
     4261 tgttgacgac ccgggactcc ggataatcga cgcagagatt gttgatacta agacgtgagt
     4321 cagtcatgtt gaggctccag aacatcgcgc aagccatcac ccagcagatt aaaggccaga
     4381 ctggcaatta agataattgc gccgggagcg gcggcaatcc accattgatc aaaaatgacc
     4441 tgcataccgt cggcgatcat ggctccccat tccgccattg gcgggcgagc accaagccca
     4501 agaaaaccca gaccagcagc cgccagaatg atacccgcta aatccagcgc cagacggaca
     4561 atggcagatg gcaggcacaa tggcaaaata tgaccagcca gcagacgcct gccgcggatg
     4621 cccatcattt ctgcggcggc aagataatca ctgtgtcgca gacgttggat ctcactccgt
     4681 gcctgacgcg cataagcagg ccaggtggtt aatgcaaggg ccagtgcgcc gttaaccagc
     4741 cccggcccca gcatggcaac aaaagcaaaa gccagaatta aacgcggcat tgacattact
     4801 atgtcagtaa aacgcatcag cacgcgttcc agccagccgc cgtagtaccc ggctaaaatg
     4861 ccaatcagca gaccgaccgg gagtgtaatg attgtcacca atgccaccag ccccagtgcc
     4921 gggcggctgc cgtagattaa acgtgaaagt aggtctcgcc cgtaactgtc agtgccgagc
     4981 cagtgctgac gactcggtgc ttgtaatcgc gccgccgcat cctgccagtt aggatcgtga
     5041 ggtgccagcc acggcgcgaa gagcgcaatt agcatcaata acaggataat tatcaacccg
     5101 caaaacgcgg cgggtgaatg gcgtaaacgg cgtaaaaaga gaaacatcgg catcagcgta
     5161 acctcggatc ggtcagccgg accagcaggt cggttaagtt atttatcaac acaaagcaga
     5221 cgccaatcag cagcgttcct cccatgattg ctgtggtatc acccgcgaag agcgctgtag
     5281 tgaggtagcg cccaatacct ggccatgaaa agacggtttc tgtcagtacc gcgccttcca
     5341 gcattgatgt ataggcgagg gcgataacgg tgagcaaggt gccgcgaatg ttgggtaaaa
     5401 catgacgtag caaaattgtc atctcgctgg cccctttggc gcgcgccagc agaatatatt
     5461 ctttattcat ttcgccaaga caggcggaac gagtcaggcg cgtaatactg gcgagcgaga
     5521 agtacgccag taataaaacc ggaagtatca aatggttgat tgcattggta aatgcctcgc
     5581 gatcgccaga aagccacgta tcaatcagcg caaatccgct gcggggttct atggtgaatt
     5641 gataaatatc atccagcctg cccggcccgg cactccattg cagtctggcg tagaacagcg
     5701 ccagcatcag cagaccaagc caaaaaacag ggacggaatt tcccagtaga gttaatgttc
     5761 gcacggtgaa atccagcgga gaaccgacat aacgggcgca aagtacaccc gcaatgacgc
     5821 cgacaacagc accgacaata agcgccaacg ttgccagttc aatggtcgcc gggaatgctg
     5881 atagcaagtc ctggagtact ggttgcccgg tggagctggc gatgcccaga tcgccgtgtg
     5941 ccagattcat caggtaatgc cagaactgga cggctaacgg ctgatccaat cctaactggt
     6001 ggcgtacctg atcataggtg gactggctgg cgtgatcgcc aacgatttgt agtacgcgat
     6061 cgaccggtga gagagccgaa agcgcaaagg taatcagcag caggccgagt agcgtcagag
     6121 caacggtaaa cagcccttgc aacagcgcga gcagagggcg tttgcctcgc catcttgcgc
     6181 gagtggaaaa aacgacagac atgatctttc catacagtga taaatcagcg gctggcctgc
     6241 aatacaggcc agccctggta ccgcgcccgg taacatggct ggacgcgagt tgcatacagg
     6301 gttatttggt tacctgatca aaccagacca tatcggcatt caacccttgt tgatacccct
     6361 gcacattatc acgcatgaca atttgcgttt tgccttgatc aataaagaca tacggggaac
     6421 tacgttgtag ttcttgttgc attttcttat acaaatcaag acgtttagtg ctatcaggtt
     6481 cagcgatggc cgccagcgtt tctttgttca gttccgggat tttccagccg tttaacccgg
     6541 caacggtgct ggatttgcca tcgttatagg cgaaagagct ggcgttggaa tgtgcgtcaa
     6601 aataatcagg caaccacata cgaatggccc cctgatgctg gtgggcgcga acgcgagagt
     6661 agacctgact ccctgcggct ggcagcaaat caacttttac accgccctga gcaaagctgg
     6721 cctgaattga ctgcgcgatg gtaataaatg gcggtttatt ttcgacatca agcgtgaagt
     6781 gcgggtcttt gattccggct ttagcgagga tctcttttgc tttggctgga ttaaaggtaa
     6841 acggtgtttc ttccagtgct ccaggaaagc ccacgggcag gaaactctga tgggtaaaat
     6901 attgtccttt taagagattt ttggtgatgc cgtcgtaatc gaccaaccag cgagcggcct
     6961 cccagaacgc aggattactc atcagcgggt tatccttgtt accggtattg aaggccaggt
     7021 agttctgttc ggcggaggca atgctcatca cttttacgcc aggtttaccc tgtaatgcat
     7081 cgatttgatc ggctcccaga tcacgagcca tatcagcatc accttgttgg atcagcaggc
     7141 gacgtgttgc cggatcggga acatttttga tgatgacgct tttcagtttc ggggcgccgg
     7201 ttggtgagct ggcgttggct tccatcacga tggcctgatg cggttggtaa gtacgcattt
     7261 taaacgcgcc gctgccagcg gaatgcatct tcagccattc attaccaaag tcgttatttt
     7321 tggcgtttgg cgcaaccagc ttctcgtcaa caattgaggc gattggtgtg gagagaatat
     7381 tcagtgccag agccgggctg acgtcggcac tccattgcac ctggacggta tggtcatcga
     7441 ttttcttcaa ctggctggcg atattttccg gctgccatcc cagcacattg agaataaacg
     7501 caccggattt attcagtgtg acagcccgga cataagagta aatcacatct tccgggcgta
     7561 ccggattacc agaggcaaat ttcgcatcgc ttttcagttt tatggttagc gttttggctg
     7621 cggggtcagc ctgccaactt tccgccagaa tcggagtaat tttcgtcgga tcattacggt
     7681 ccggttgcac tacgcgttga tacagacttg gtacagtctg aatacttgag agttcattag
     7741 cttctgccgg gtccaggctc acaatatcat ccagcccctg ggctacaacc aacgtattag
     7801 gcggtgtagc ggcaacggtt gtggcggaaa ttgtgcttaa aaccagtaaa ggtaatagtt
     7861 ttttaatcat atgcttccca atccctgaaa ttactttgag gttattaata ttctatcaga
     7921 cggggttttg ttgcattaat cgcgccaaaa tatcccgtta atagaacgag ttaaacggtc
     7981 gatgatttct caccttaccg aaatttggtt catcaaatgt aaagtattat tacgttaggc
     8041 cgcgatgaga attgagtaaa gataattctc atttgcaatc gtgactggaa tagaagaaca
     8101 accgagaaga cgtcgctaat aaccaggata ggctggtttg actgcgcagg tatgtataaa
     8161 aaaatccgga aacagagagt cgtttccgga ttgatacaaa ggcgttgggt aatatcaccg
     8221 cttttaacgt gagtttaagc ggtattacgc cagtgccgct tcgcgcaggc gagttttcaa
     8281 cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga tcggctcgac
     8341 gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt tttcggtcac
     8401 cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc ctttgttcgg
     8461 acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca cccaaaccaa
     8521 tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg cgtagccagg
     8581 ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc aagagtagtg
     8641 accaacttca cgcaccgttg acagtaccat cgggtattcg tcggtgagtt tgtcgattgg
     8701 cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa acttctcttt
     8761 aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa
     8821 gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa
     8881 ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac gagtggcaat
     8941 ttcactgatg atttgccagt ccgttttcag atcccacttc ggctcaaccg ccttgaagaa
     9001 acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc acgacgttga
     9061 cggtaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct gaacgataac
     9121 cagttccaga tcttcaaagg ctttacgtac tgccgacagt tccgcgtcag tttgcagcgg
     9181 atcttcgccc ataatgtacg ccgctctcac ttcaccatgc gccacgcggt gcggcagctc
     9241 gctgatacga taaccggtat gcgctggcag gctctctacg ccccaggctt tggcaaattt
     9301 ctcgcggtta gccggatctt tcacgtactg atagcccgga tacgtatccg gcagcgcgcc
     9361 catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac ccgcatgtgg
     9421 cttaccgagg ttaccggtca gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc
     9481 ctgatagaac tgggttaccc ccatgcccca caggatggcg gcgctttctg cctgggcata
     9541 catccgcgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt caaccgactc
     9601 cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac gggaagcgac
     9661 gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat tcaacagcgc
     9721 gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt
     9781 gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta cgtgattcgc
     9841 cacgattggg tgggaatccg ccgggttata cccgaaaacg aacactaaat cggtattatc
     9901 aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca gacctgcaac
     9961 cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg
    10021 cgcaaatttt tgcattacat agttggtttc gttacccgta ccgcgcgagg agccggtcgt
    10081 ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg caacgtaatt
    10141 cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac ggatcatggg
    10201 ggttttcagg cgcggggtca ggatctgggt atcgttaatg aagtcccagc cataataacc
    10261 cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc ggacgatttt
    10321 gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc aaaccgtgac
    10381 gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct ctcgactaca
    10441 gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac gggcactatt
    10501 tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga aacgaaatac
    10561 cgcgtgacag ccatcacgcg gtagacattt tattttttct cgacgacggg agccgcctga
    10621 tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg gctgtccagc
    10681 atcagtaatg acatctcttc ggcaagcact ggcaaccggg cttcggtggc ctgtaagcgg
    10741 ctggttaagc cgcgttggta ggcggcctcg gctgcgcgct gggtgaagcg cgttgcttct
    10801 acgcgttcag cctgcatctc tcgctcgtcg ttgagcgttt gcagacgtgt gccgttgacg
    10861 gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat catgttgctg
    10921 gcggcgcgag tgccttcgag attggcattc aaccgtccac cgtcaaacag cggcaatttc
    10981 agacctggga taaagttgaa ctggcgactg gtttttttga ataaggtatc caggtggata
    11041 gaatccaggc cgaaaaacgc tttgatatca aagctcggat agaacagcgc ccgcgcggaa
    11101 tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg
    11161 gcgagcaact cataagagag tgtcgccgga ataccggtct ggacttgcgg taatgccacc
    11221 ggtttgatct ccggcatatc gctggctccc gcgccaatca atgcacgcag agattctcgc
    11281 gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag aatctgtgcc
    11341 cgcgcgccgt ggaaaggcac ttgcgcttcc agaccgtgcg ccactttact ctggtgcgct
    11401 ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat ctgatagctg
    11461 gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc tactgctgcg
    11521 gtttctgcca gcgcggcatt atgcgcgcta atggcggcag caaccgctga gcgatgcaca
    11581 ccccacaaat cgagatccag tccggcaaac aaaccgactg tggcttccgt atagtacggc
    11641 ccgtccatac ccagtgctgg cgcatccatc gcataagggc ttaaaaagcc gttcgccgag
    11701 acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc ggcatctaac
    11761 aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa
    11821 ccatttagag tccgttggat cagcgcatcc agctgcgggt cattgagttg tttccaccac
    11881 tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt gatttgttcc
    11941 ggtttgagct gttgatgagg cgcagaatcc ttacgtacca gggcacagcc ggaaatcagc
    12001 gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt gatcattgtg
    12061 tggctccttg tgcctgttcc ttggcgggcg ttaatgccgg ggctgtccag tctggcaagc
    12121 gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg atggcctgcg
    12181 aggcgggtgt ttcttcaagc gttatttgag gagaacggct gagtgcggtt gccagaccag
    12241 cggcgtattt ctccagtgcg tcggcaaact gacctgctct gtccggctgt agtgcgttat
    12301 ccagcgcctg cgccgagttc caggtggcat cccaggcgtg aagaatatcg cggccctgac
    12361 gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct
    12421 ccagcaccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc agacctatgc
    12481 gaatttgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc cgcattactt
    12541 tactcaacat acccagcgcg ccagccagtt tttgcggcag cgtgcgcgct tcgctttcag
    12601 gccagacaaa ggtgtaaatc accgccgaca ccacggtacc aatgatgata cccagagcgc
    12661 gatcgcgaat ttccaccagg tcgtacaccg ggccaaaaac gttttcgagc gtggcgagcg
    12721 cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg gtggcaatcc
    12781 atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata ttgtccagcc
    12841 agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag gccccgccaa
    12901 aacgcagcac catcttctgg tacgacgaac cgacatttgg attagcgacg atcacgcagg
    12961 tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtagaaggtg taacagatca
    13021 aacaggcgag cagcgttttt accgcgtagc gcatatagtc tggattggta aaagcgtcgg
    13081 cgactattga tggcggtttg gtggcgggcg ttggcggcgt attcgggtcc atctgaccca
    13141 gttgcaacaa tgtctggcag atattctcca ggttacattc ccgtgccgcc atcgcttcac
    13201 tttcagtgat ccgccagtcg ctttgccagc actgaccttc ggcaatggca tgctgcagtt
    13261 tgttgatttc tgaggctaat ttttgtcgga attcaataat tgcctgagaa tcagcaaaag
    13321 aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt
    13381 gccaccatgc gctctgagtt cgccagttgg catcgtccgc gaggcaaaag acattgagtt
    13441 tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg
    13501 tcaggtggct aatggcatca tcaagccgat cattaagcgc ctgatgcatc tgcgtgatag
    13561 cgcggctggg aaaccacagc acgccgatta acgtcatcaa taacgtcggg tagaggccga
    13621 caacgataca ccacagggtt aagcgcacga ccacttccgg atagtcgagc atggcgggga
    13681 aggtttgccc gtaaatagcg acaatggcga cggcgaaaaa gaccagcccc agccgatggg
    13741 tgcgcatcaa aaacatgcag cccatcagga tcggcccggc gatgatcaat cggatcaacg
    13801 gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc accgtggcaa
    13861 ccacaaacaa gatcgccaca aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca
    13921 gcactgccag cgataacgcc acaaaaggga tctcaaaagt catcgagatc agaatcacca
    13981 gtaggcagcc tacccagagt tgcacggtct gcggcacacg acctggccgc cgctcgctta
    14041 actcttcatg aaagaacgcc agcagcctga ccaccggtaa tggcagggag ttgagcgcgc
    14101 tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca tttccgggtc
    14161 aggtttatca accatgattt tgaccggaaa acgctgggca acgcggaccc agttaataga
    14221 acgcgacact ttcggcaggc cgcccagcac caggccgccg tcatccggta gcacgccgta
    14281 gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcgctcatca ggcgaatcgt
    14341 tgcgggtgta cctgagcgaa tatttttcag atcagtttcg cggaaattgg cgatcacgta
    14401 ccagtgacga gtgtcgatta gggtgaaaat agggcgcatg gcagaagcaa attgcccgac
    14461 ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg ccatttccag
    14521 atgcagtttg gtcagggcaa tatccgcttc gaccgccgca cgttgggcga ccagcgcatc
    14581 gacgccgctg acggcactgg cggctgactg cgcctgcaac aatacggcat taaggtccgc
    14641 ttctgccgca cgctgggcgg ttcttgcacg gtcaacctct tccgcagaaa caaaaccttc
    14701 tcgcagtagc ggttcggtac ggcgtaacgt atcgctggct tgtttcgcgg cggcacgggc
    14761 tttttctacc gtggcattaa ccgagtcggc accaaactgt tgcgcgtcaa cgctacgctg
    14821 ggtgagcata atttgcttat ccagcgccgc gagggaggct tcagctttcg ccagattggc
    14881 ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc tgtttcaccg cctggttgtc
    14941 gctgaccgcc agttctacaa tgcgaccgct gacttccggc accacatcaa tggtatctgc
    15001 tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg cgccagataa cgaaaacaag
    15061 ggcaaccagc gccaacgcaa ccactaacag agcagggaat ttactgcgag gagctttttt
    15121 cggcgtactt tccatgagcg cgtctcaaat tgattagaaa aatgccagcc agaacagcat
    15181 ggcgtagaga gcaaaaaggg cggtataaat aattccgaca aatgccagat tgatatttgt
    15241 ccgctgaata attcgcctcg tgatgagcgt caaaataagg ctggcaatgg cgcagaaaaa
    15301 ccagccggga taataagcgc cgatcaccgg gattgccgga gataatgagc atcccgaaac
    15361 catgaagacg ggaattatcc aggctatttt tttgagttgc atcgctatgc gagagagaac
    15421 tgttggcata ttaaatacat cctgtctgct gcgaacaata tctgtagtga atatcaatat
    15481 ccattgaagg cgtcagtatg gctattttct cgatgaataa aaatagcttt attcagccac
    15541 cgcattcata acttatatat attatgtatt aattttctgt atatattttt aatataaata
    15601 ctttcgggag cgtttacgcc cccaaaagcg agtgatatta atttggggtt acgatattca
    15661 cccacaaatc gaaattatcc agacaggcga taatttcttc cagtttcgca ccattgccaa
    15721 taattttggc tttcttcgct ttcaccagat cagccatttt tgcctgtccg gtcagcacgg
    15781 cgtgcagatc ttcacggctg atgtagaaag aggcgtcagc ttgcggttgc agtgttttac
    15841 ggtagttaag cacgctatcg ttcagcgtca ggttgaggtt atcgccgttg ttcatattga
    15901 agttcaggct gatattttta cccgcggctt tcgcgctatc gaggcgaacg gacataaagt
    15961 cgaacagcat ttcgaccgac atcccacgaa tggtgtccgg ggaaccggtg gtgccgtggc
    16021 tgaacttatg caccccttcg cgcagctctt tcgcgccggt caggtagaaa ccgcgccatg
    16081 tggcggactc ggcctgatag cccagctgtt caaagttatt cgcttgcaga ttcttcgcga
    16141 cctggtcacc cgggttggcg gcaatcacct gtttcagcag ttctgccgac cagcgataat
    16201 caccttgctt gttcgcttct tgcgccaggt tgatgacgcg ggcagaaccg cccagcgcct
    16261 gcacgtaacg tttacccatc tccacctgac catacggatg caggttagcc gggttaccgt
    16321 cgtaatagcc aagatagaag ttatataccg cgcgggcgtt gtggctgaca gaaccgtaat
    16381 agccgcggct ggcccagtta ttggcaagcg caggcggcag cttaatcatg tcgccgattt
    16441 cattcatggt gtagccctgg ttcgccaggt gcagggtctg gtcgtgaatg tacttgatag
    16501 tatcgcgata tttaccaata taatcattga tatgcttatt gccccagacc ggccaggtgt
    16561 gcggcataaa cagtacttcc gcgtcgttac cccacatatc caatgtttcg ttcagatact
    16621 cggtccactt gctggtgtcg cgggttttcg cgccgcgcag ggtgtagaag ttgtgcaggg
    16681 tatgcgtggc attctcggcg gtacacaggg ctttcagggc cggaatatag aagtgcattt
    16741 ccgctggcgc ttcgctacct ggggtcatca ggaagtcaaa ctccaggccg tcgataatca
    16801 tcttctcgcc agttctgacg atagttttag tcggtgcaat aatgctcggg tcgcccgttg
    16861 ccagcgtcac gccgaggccg ttgcccacgt tgccttgcgc gttgtgcggc agtaacagac
    16921 cgtaagagta gagcgcacgg cggctcatga tgttacccgc cagcacgttt tcgctgatgg
    16981 cttcgtccat aaagccagct ggagcaatca cctgaacttt gccggattta acatcggctt
    17041 cagagataat acctttcacg ccgccgtagt ggtcggcgtg gctgtgagtg tagataacgg
    17101 caacaatcgg tttttgcgga cgatgctgga agtaaaggtc aagtgcggct tttgcggcag
    17161 gtggcgtcac cagtgggtca ataacaataa tgcctttctc gccctcaacg aacgtaatgt
    17221 tagagatatc ctggccgcgc acctgataca ttttatcggt gactttgaac aggcctgaaa
    17281 tgccgttgat ctgcgactga cgccacaggc tggggtttac ggtttccggc gcagcggcat
    17341 taatatcaaa tttgtaatca ttcgcgcggt agtaaatttt accgttcgca tcacgcagaa
    17401 taccttcatc cagcagcgga gcgataaatc cacgctgagc atcgtcaaaa tcctggcgat
    17461 cgctaaacgg taatgatttg gcgtaattct gattaacctg ttgggtatat tgagtggcgg
    17521 ctttcgcctc atctgttgcg taagcggtat ttatcatcat gccgctggcg gcagtgaacg
    17581 caataacaat cctgctcaaa cggaataacc gagagttatt catataaatc tccatttatc
    17641 atttatgtcg taaatatgta atccatgcaa aatacgtcat ttaaagctgg cgttatcata
    17701 attgggaatt tgcgacttta ataagtggaa gtgtgagcgg aacgagccat tttattaggc
    17761 atttgtgctg aagagggaaa attacagtag aactatcatt gctgggaata atatgggctg
    17821 ttttatgtaa ttattatata tattgtacct gtcgttggca cagcgggaca ttgcgctact
    17881 gtgccaacgc tttactcatg ggactttagc acaggactgt ggcaaaaaac gttgatgcgc
    17941 caatattgct gcaccctgag cgccattaaa gtcagaagat gaggcggcaa taaagcgcac
    18001 gacctgatac ggcagtggac ggcgcaggta cttttgggtc atggcaatga gagtctcgcg
    18061 tgggaaggcg ggcatatcca tcacgccacc gcccagaatc accgcatcgg gatcgaacag
    18121 attaatgctg gtggcaatgg ctcgtgccgc gttttcaagc agactctgga cgaaaggggc
    18181 gttttccgca tggacgaaaa gatcgctcaa tgggtaattt cggggctgtt gttcgtacca
    18241 gcgtcttagc gccattccag agcaattggt ttccaggcac ccagaattgc cacacgcgca
    18301 gtgttgggtc atatctccca gggggatatg gcccagttcg cctgccacac cgtgtgcacc
    18361 cgtccacggc gcaccgttca tccacactgc gaaccccatc cccgtaccga gataggccgc
    18421 cagaaccagt tgttgcgtaa ggcggttttc tactacgtcc caggaaagtt gcaggttaac
    18481 gtcgcgggaa aactcaaccg gacaattcag cgtattttcg agcttatcgg cgagatcata
    18541 taaatctgcc gctgttaacg gcaggttagg cgtagaaata atggtgcgtt tatctttact
    18601 gaccagcgcc ggaaatccca tcaccagacc atgacagcga gcgttaaagc gcctgagttg
    18661 ctcgtcaatc atttcgccga tacccgacac caggtcggga gcaataactt ctgcggtccg
    18721 ctttttttcg cagtgtagcg tttcaccttc tgctgtccgc agacaaaagc ggatatgcgt
    18781 tgcccccata tccacgcccg ctacgacgtt atgctgtttt tgcatgaggc tgtacctcgc
    18841 tttttgcagc cagaatctgc gcggtcataa ttctccatgc ttcgtcgata ttttccgcat
    18901 gattaaacag gccggaagtg ccgacgataa agacatccgc ccctgccgcc attagttttt
    18961 cgtaagttgc ctggttgcag gaaccgtcca cctcaatttc gtactccaga tcttttcgtt
    19021 cacgccatgc cttcagttcg gcaactttat ccagcatttc aggaatgaag ggttgtccgg
    19081 caaaaccggg atcgacagtc atgaccgtaa ttttatcagc cttatggata tagtatttca
    19141 tggcctcaac cggcgtttcc ggattaagaa tcagccccac tttcatgcca tgacggcgga
    19201 tttcatcaat caggcggaat gcctgaccgt tgatagtttc cggatgcaaa gtgatgaaat
    19261 cagctcccgc acgtgccagt tgagcaatgt aatcctgcgg acgcgttacc atcagatggc
    19321 agtcgagcgg tttactcgcc agttttttta cctggcttac gaagaacggt gagagcgtca
    19381 gattcggcac aaagtggccg tccatgatat cgatgtgaaa gtaatcggca tggctgtcga
    19441 taaattcgat ctgttcttta aatttcagca gatccataca cattaacgag ggggagattt
    19501 tcatgttcaa ttccttactt actgataaga cggtcaaggg cgacagccgc gataattaat
    19561 ccgcccatca ccaccagttg gtaataggtt tgtacctgca aaatattcag accgttgttg
    19621 atggtgccga tgatcaaccc gccaatcacc acagagaaaa tgcgcccctt gccgccgaag
    19681 aaactggtgc cgccaatgat ggcgctggca atggcatagg tttcaaaacc cataccggca
    19741 agcggttctg cggcaccgag tcgtgcagtt gagacgacgc ctgccagacc tgcacaaaca
    19801 ccggagatga taaacaccac caggatgtgg aatttcacgt caatcccgga atagaacgcc
    19861 gagtttttgt tgccgcccag tgcgtagatg ttgcgcccga gccgcatacg cgttgtcaga
    19921 aaccaaagga tgagcgcgac aattagcgag aagataacgg ggacaggtat cccaattacg
    19981 ctggcggcaa agaagttcac gaagtcaaat gagaagccgt ataccgagtt ggcatcggag
    20041 atcaccagcg tgatcccacg gaaaatagcg ttggtgccaa gggtgataat gaacgggtgt
    20101 agtcccgtcc agttgaccag gcagccgttg atcgccccca gtgcgccgcc aaccagtaca
    20161 ccgccaatta gcgccgcgag aaacggatca atacctgcca acatcagttt agcggtcacc
    20221 ataccggaaa gcgccagaat cgcgccaacc gaaaggtcga taccggcgac caggatagcg
    20281 aagaattccc ccatgccgat cagtaccgtc acggagcttt gcacaaaaat ctgggtaata
    20341 ttattggtgg tcagaaaata ttctgacgac agcgaaccaa aaatggcgac gatgatcgcc
    20401 aggataaaaa aagtaccgta tttatcccag aacagcgcaa agttgaacgg tttcttctcg
    20461 ctcgcttcgc tttttactct tgtggtaaag cccatgccat aatctcctct tcgctcatgt
    20521 catcgcgatt cgtcaggatt tgcgtcagtc gtccttcgca gaacacggcg atgcggtcgc
    20581 agacggtgat aatttcaggt agttcagatg acaccatcag gatgactttt ccgtcgtccg
    20641 ccagttggcg catcactttg taaatttcgg ctttcgcgcc aacgtcgatg ccacgggtag
    20701 gttcatcgaa aatgatcact tccgggcgac agcacagcca tttggagatc aggactttct
    20761 gctggttacc gccggagagt tcagtgatgt tctggtttac cgaatgacat ttcagcgcca
    20821 gcagttcgcg ttgattttca gcggtacgtt gctcgtcaac ttcatgaaac aaccccatcg
    20881 cgcctttata gccgccgtct ttcagactgc ggctgatagc catgttctga gcgatagaaa
    20941 agttggggaa aaagccgtta tcccggcggc tttcagtgat gtaagccatc ccttttttca
    21001 cggcatccag gggagaacgt ggagagatat ctttgccatt aagacggatt tctccgccag
    21061 cgcgtttatc aacgccaaac aggcagttca tcagttcagt acgtccggaa ccgaccagtc
    21121 cggcaaagcc taatatttct ccccggcaga cgctaaatga gatatcccgg accttttttc
    21181 tgtcacggct ggtgacgttg cgcacctcaa aaaccgtatc gtgcgcaagg ttgctgacat
    21241 tctccttcat cgcgttaaaa cggttttgca gttcgcggcc taccatcaga cggacgatat
    21301 cgtcatttga cacatcgctc accatgccgc tgcaaacgct gctgccgtct ttcatcaccg
    21361 tatagcggtc gcaaatacgg cgaatttccg ccaatttatg cgagatatag acgatagccg
    21421 taccctcctt acgcaactga ttcatgatca gaaacagata gtccacctct ttattggtga
    21481 gtgaggaggt gggttcatcc atgatgatga ctttggcgtc gagcatcagc gttttggcaa
    21541 tttccagcat ctgcttgtgg ctgatagata agttcgccac tttctcatct aaatcaactt
    21601 tcaagcccac gcgtaataac atcattgcgg cgcggacacg catttctcgc cagtcgataa
    21661 tattgacacc gcagattttt ttcgtcagat gacgaccaat atataaattt tccagtacgg
    21721 ttaattcatc aataacgctg agttcctgat aaataatccc gataccgagt tgtgccgcta
    21781 atttatgatc cagtttgtta tagttaatgt tattaatggt aatggtgcct ttggttggct
    21841 catgtattcc ggataacact ttcattagcg tggatttacc cgcgccattt tctcctagta
    21901 atgcatgtat ttcaccagga taaaccgtta aattaaccga ctttaatgcg tgaaccggac
    21961 caaaggactt gccgatcccc gccatcgata tatatggcgt ggccataaat aacctctctg
    22021 cacgaaatat cgtattattg cctgatgggc tacacttatc aggtctacgt tcaggttatt
    22081 ttgttggccg gataagacac ttcattagca tcactatccg gcatcattta gttttattga
    22141 gtgaccagga ttgaatcgac cagtttaaat tccggtgctt tatccagcgg gataaccttg
    22201 ccggattttt cagcgtcaac catcagcttt agacccgtag cgccgatatc cgccgggttc
    22261 tgggcaaccg tcgcggtcat ttgtccggct tccaccattt tgcgggcttc cggaatgcca
    22321 tctgtaccga cgaccagcac ttttcccgtt tttccggcgt ttgcgacagc ctgagcaaca
    22381 cccattgcca tcgtgtcatt cgcgcaatag atcgctttaa tattcggatt acgttgcaac
    22441 acgttagtgg cgacatccag tgctttaatg cggtcccagt cggcaggctg gctggcgaca
    22501 agcttgatct ggcttgcttt tttgaaggtt tcggtggcac cattacgacg cgcttcaccg
    22561 gaggcgttac cggctttacc ttcaataatt gcaacttcac ccccttcggc acccagcttg
    22621 tcaataatga atgacgcgcc tttcgccccg acggcaacgt tatcggtggt gacaaaacct
    22681 tccacattac cgcctgcttt tttcagatta tccatgtcga ttttttcatc gagattgacc
    22741 agataaatgc cttttttcca tgcgcgggcg acaggcatga ccaggttcac tgaggataat
    22801 ggtgcgaagg cgataccttt gtaattttta ttactgagat cttcaaataa ctgcaattga
    22861 gattgaaaat cgccttctga aggagaggca aaaatatcaa cgctgacgcc cagcgttttt
    22921 gcttcatctt caatgccttt tttcatatct acccaaaatg ggttggagag tgttttcaat
    22981 acgacggcat attcggcggc agcaaaagcg ctggttgaca acattaagcc cacgagtgtg
    23041 ccgctgaaat atttcagata tttattcata atcatgttct cacggtaagg ggataggtaa
    23101 ctccgcgatg gcggaattac ctttttctta tttcagcgcg cctggtgaga agaaatcaac
    23161 aattgcgccg gttttttgca tattaatatt agcttgttca atgttgagct gggcgacaga
    23221 gacaaaaaac gcgtccagca aagttagttg taatattctt gccgaggcat tacgacctaa
    23281 taacggtgtt tccggggccg gtgagcaaat aatataatcg gccagcttcg ctatcggtga
    23341 atggtagcta tgcgttatac aaataatctt tgccccgttc ttttttgcca gttctacagc
    23401 tgcttttaca tcactggttc gcccggaatg ggtcaccacc agcaccacat ctccttcctg
    23461 taacaacgaa gcagacatca tcatgatgtg agcgtccgga taggcctggc agcgtacgcc
    23521 aatgcgtaaa aacttgtgct gtacatcagc acagattgca tttgatcctc cggcaccgta
    23581 caaatcccgc tgtctggcct gatagaaaaa gcgggcggca cggtggatct catcaacgtt
    23641 gacgatcgac tgaccttcca taatggtgcg taaagtgatg ttaaatacct tattcaccac
    23701 atcctgcggc gcttcatcaa aagccaactc ggacggcaat acttgttctg actgagaaaa
    23761 ataatcttcc agcgcactgc gtaagttacg aaagccgcta aaccccagca gctttgatac
    23821 cttaacgatc atcgcttcag ataccgccag agcttctgcg acatctttaa ttgcgggtgc
    23881 acaactcagg ttaccgggtt tgagtaacca ctccacgatg cggctttcgt tttctgtcat
    23941 tccttcctgc ttcattcgca ggtaaggcgc taacccgata ccgttcggaa gcgctgaatc
    24001 aaactctgac tggctcatca cgttctcttg tccttgaatt gaagacgcca ctatatcacc
    24061 acagcaagaa cagaaa
//
LOCUS       DAPQZD010000064        24054 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPQZD010000064 DAPQZD010000000
VERSION     DAPQZD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.064"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            219..983
                     /gene="nfeF"
                     /locus_tag="RO454_002487"
     CDS             219..983
                     /gene="nfeF"
                     /locus_tag="RO454_002487"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDY0934306.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
     gene            complement(1037..1112)
                     /locus_tag="RO454_002488"
     tRNA            complement(1037..1112)
                     /locus_tag="RO454_002488"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Ile,seq:cat)
     gene            1237..1743
                     /gene="mug"
                     /locus_tag="RO454_002489"
     CDS             1237..1743
                     /gene="mug"
                     /locus_tag="RO454_002489"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDY0934307.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(1821..3662)
                     /gene="rpoD"
                     /locus_tag="RO454_002490"
     CDS             complement(1821..3662)
                     /gene="rpoD"
                     /locus_tag="RO454_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDY0934308.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(3857..5602)
                     /gene="dnaG"
                     /locus_tag="RO454_002491"
     CDS             complement(3857..5602)
                     /gene="dnaG"
                     /locus_tag="RO454_002491"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDY0934309.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(5713..5928)
                     /gene="rpsU"
                     /locus_tag="RO454_002492"
     CDS             complement(5713..5928)
                     /gene="rpsU"
                     /locus_tag="RO454_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDY0934310.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            6166..7179
                     /gene="tsaD"
                     /locus_tag="RO454_002493"
     CDS             6166..7179
                     /gene="tsaD"
                     /locus_tag="RO454_002493"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDY0934311.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(7222..8685)
                     /gene="ttdT"
                     /locus_tag="RO454_002494"
     CDS             complement(7222..8685)
                     /gene="ttdT"
                     /locus_tag="RO454_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDY0934312.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(8733..9338)
                     /gene="ttdB"
                     /locus_tag="RO454_002495"
     CDS             complement(8733..9338)
                     /gene="ttdB"
                     /locus_tag="RO454_002495"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDY0934313.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(9335..10246)
                     /gene="ttdA"
                     /locus_tag="RO454_002496"
     CDS             complement(9335..10246)
                     /gene="ttdA"
                     /locus_tag="RO454_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDY0934314.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            10453..11385
                     /gene="ttdR"
                     /locus_tag="RO454_002497"
                     /gene_synonym="dan"
     CDS             10453..11385
                     /gene="ttdR"
                     /locus_tag="RO454_002497"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDY0934315.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(11398..12015)
                     /gene="plsY"
                     /locus_tag="RO454_002498"
     CDS             complement(11398..12015)
                     /gene="plsY"
                     /locus_tag="RO454_002498"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDY0934316.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            12120..12488
                     /gene="folB"
                     /locus_tag="RO454_002499"
     CDS             12120..12488
                     /gene="folB"
                     /locus_tag="RO454_002499"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDY0934317.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12579..13400
                     /gene="bacA"
                     /locus_tag="RO454_002500"
     CDS             12579..13400
                     /gene="bacA"
                     /locus_tag="RO454_002500"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDY0934318.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGENKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13480..14718)
                     /gene="cca"
                     /locus_tag="RO454_002501"
     CDS             complement(13480..14718)
                     /gene="cca"
                     /locus_tag="RO454_002501"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDY0934319.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVSLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDSLFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(14782..15402)
                     /locus_tag="RO454_002502"
     CDS             complement(14782..15402)
                     /locus_tag="RO454_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDY0934320.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINRLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            15644..16945
                     /gene="ygiF"
                     /locus_tag="RO454_002503"
     CDS             15644..16945
                     /gene="ygiF"
                     /locus_tag="RO454_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDY0934321.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            16968..19808
                     /gene="glnE"
                     /locus_tag="RO454_002504"
     CDS             16968..19808
                     /gene="glnE"
                     /locus_tag="RO454_002504"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDY0934322.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     ALLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            19856..21289
                     /gene="hldE"
                     /locus_tag="RO454_002505"
     CDS             19856..21289
                     /gene="hldE"
                     /locus_tag="RO454_002505"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDY0934323.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(21409..21468)
                     /gene="ibsE"
                     /locus_tag="RO454_002506"
     CDS             complement(21409..21468)
                     /gene="ibsE"
                     /locus_tag="RO454_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDY0934324.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(21708..23369)
                     /locus_tag="RO454_002507"
     CDS             complement(21708..23369)
                     /locus_tag="RO454_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDY0934325.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            complement(23396..24025)
                     /locus_tag="RO454_002508"
     CDS             complement(23396..24025)
                     /locus_tag="RO454_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDY0934326.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
BASE COUNT         5569 a         6096 c         6576 g         5813 t
ORIGIN      
        1 aatgattttt aaacgcaggt ttactcaaca tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcattga gcaatgatgc ttgataagag gttgcgcgtc ttatcagact acagatatat
      121 cagtgctaca aatcacttgc atttatcatg attaacaatc attatcattt gtggaattta
      181 tttagatata tctgattacc atcacgaagg cgataacaat gaataacacc ccccgctacc
      241 cacagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca
      301 tcagcgccgg ttttcagcgc attgtcctcg gcggcgaggc gctggacggt tttacatcgc
      361 gcggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac tttgtgccgc
      421 caacggtaac ggaagagggc atcgtctggc cggaaggatc acgcccaccg tcgcgtgact
      481 atacgccgct gtatgacgaa ctacgccatg aactggcgat tgattttttt attcacgacg
      541 gtggggtcgc cagcggctgg gcgatgcagg cgaaaccggg cgataaactt acggtggcag
      601 gtccgcgcgg ttcgctggtg gtgccggaag attacgcgta tcagctgtat gtctgcgatg
      661 aatccggaat gcccgcactg cgccgccgcc tggaaacgtt gagcaaactt gctgttaaac
      721 cgcaagttag tgcgctggtt agcgtgcggg ataacgcctg tcaggattat ctcgcgcacc
      781 ttgatggttt taatatcgaa tggctggcac atgatgagca ggcggtagat gcgcgtctgg
      841 cgcagatgca aatccctgcc gatgattact tcatctggat aaccggcgaa ggcaaagtcg
      901 ttaagaattt aagccgccgc tttgaaacgg aacagtatga cccacagcgg gtacgtgcgg
      961 cggcttactg gcacgcaaag taacggatat ataaaaagaa aaaggctgac gatttctcgt
     1021 cagcctttgc tatgtctggt ggcccctgct ggacttgaac cagcgaccaa gcgattatga
     1081 gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa gtaactccgt
     1141 gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat tttcaatccg
     1201 ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat tttggctcca
     1261 gggttacggg tcgtgttttg cggtatcaac cctggtcttt catccgccgg gactggtttt
     1321 ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg gtttaccgac
     1381 cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg cgtcaccaaa
     1441 ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct acacgcaggc
     1501 gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc gattctgggc
     1561 aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa acaaacgctc
     1621 accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca
     1681 ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtggt gcgtgggcga
     1741 taaaaacgcc accgaaaagg tggcgtttgt gcggagggac tagtgccggg tgcggcgtaa
     1801 cacctgatcc ggcctaccga ttaatcgtcc aggaagctac gcagcacttc agaacggctc
     1861 gggtgacgca gtttgcgcag cgccttcgct tcgatctgac ggatacgttc gcgggtaacg
     1921 tcgaactgtt tacccacttc ttccagcgtg tggtcggtgt tcatatcgat accgaaacgc
     1981 atacgcagaa ctttcgcttc acgcgcggtc aggccagcca gcacgtcgtg cgttgccgca
     2041 cgcaggcttt cggtggtcgc agaatccagc ggcagctcga gggtggtatc ctcgatgaaa
     2101 tcccccagat gcgaatcttc atcatcaccg atcggcgttt ccatggagat tggctctttg
     2161 gcgatcttca gcactttgcg gatcttgtct tccggcatca gcatgcgttc agccagttct
     2221 tccggcgttg gttcacggcc catctcttgc agcatctggc gagaaatacg gttgagcttg
     2281 ttgatagtct caatcatatg caccggaata cggatggtgc gcgcctgatc cgcgatagag
     2341 cgggtgatcg cctgacggat ccaccaggta gcgtaggtgg agaacttgta accacggcgg
     2401 tattcgaatt tatcaaccgc tttcatcagg ccgatgttac cttcctgaat caggtcaagg
     2461 aactgcaagc cacggttggt gtatttcttg gcgatagaaa taaccagacg taagttcgct
     2521 tcaaccatct ctttcttcgc acggcgggct ttcgcttcac cgatggacat acgacggttg
     2581 atatctttaa cctgctcgat ggtcaggccg gtttcttctt caatctgctg cagtttctgc
     2641 agggcgcgat gcacttcttc agagacatca tgcagttttt ccgaccacgg cttgttcatc
     2701 gcaattgccg cgttgaacca ggtatcgctg gtttcgttgc cggtaaacag ggtaatgaag
     2761 tttttcttcg gcattttgca ctgctcaacg cagagcttca tgatcagacg ttcttgcgta
     2821 cgaacgcggt ccatcatgac gcgcatgctg ttgaccaggt agtcaaactg cttcggcacc
     2881 aggcggaact gtttgaatac ttcagacagt ttcaggatct cttcctgagc ggcagcgtga
     2941 ctgcggcctt tcgctttgat ggtgtcacgc gttacaatgt actgagcgcg cagttccgca
     3001 aatttttcgc gagccagttc cgggtcgatg ctgttgtcat catcggcggt atcgtcgtca
     3061 ccatcttctt cgtcttcatc ttcgtcatcg tccagatctt cctgggaaag ctcagaaccg
     3121 acgtgagtgg cggtaggtgc cagatcttct tctgcgttcg ggtcaacaaa gccggtgatc
     3181 agatcggaca gacgcgcttc ttctgcttca acacgatcgt actgttccag cagataggtg
     3241 atcgcttccg gatattcagc aacggagcat tgaacctggt tgatcccgtc ttcaatacgc
     3301 ttagcgatgt caatttcgcc ttcgcgggtc aacagttcaa cggtgcccat ttcacgcatg
     3361 tacatgcgta ccgggtcagt cgtgcgcccg atttcagatt ccacgctgga aagcacctgc
     3421 gcggcggctt cggcagcatc ttcgtccgcg gtgttttcag ccagcatcag atcatcggca
     3481 tccggtgctt cttccatcac ctgaatgccc atgtcgttga tcatttggat gatgtcttcg
     3541 atctgatctg aatcgacgat atcttccggc agatggtcat tgacctcggc ataggtcaga
     3601 tagccttgct ccttaccacg ggtgacaaga agtttcagct gtgactgcgg gttttgctcc
     3661 ataagacggt atccacactt atttcatgag gttggtgttg ggcgattgac ccgatgtcct
     3721 tcagccgtta acgctgaaat ccaacggttg tttaccgcca acgataatta cgagggcgta
     3781 cttatatatt tgccgctgcc tctcagtgcg gctgtcgggg gcttcccgat cgctcttcgg
     3841 cacttaagcc gttaaatcac tttttcgcca gctcctggtt taatgtccag agctccaggc
     3901 gttcttcgtt gcttaaacca tgcgtgcgct cacgagcgat taactcttcc tggcgcagtt
     3961 caagcagcga atcaaacata tggttgagtg agtcggtgaa ggtttgctca gcaatattct
     4021 tatctgctat atcgtcccac atcgacagtt tttcaagggt ggcagcatta tttgtaccac
     4081 gatagtgctc taaaagttgc ccggtggtca gacctggctg ggagagacaa gtgttgacca
     4141 gttctctgaa taagccaagt ccagggagct tattttcatc cagattctca agcggcggga
     4201 ccaacgtcgc taattctgga ttttgcacca gcaaccctat aagtatacgc atggtcgtgc
     4261 gttttagctg cggaacaggg cgagaaacgc cgctctctgc cgcttttggc attaatcgtt
     4321 caagctggct gtcatcaagt atgcctaatt tgttgcctaa ttcctgacga agatatattc
     4381 gcagcgtttc gcccggcact tgcgatatca gtggcagtgc cagcgtactc aaacgtgcgc
     4441 gcccgtcagg ggtactcaga tcaacttgcg gcatcagact gttaaacaga aatgcggaga
     4501 gtggcatcgc ctgctccatc cgcgcttcaa acgcttcttt accttctttt cgtaccagcg
     4561 tgtcagggtc ttcgccatcc ggcaaaaaca taaagcgtag ctgacgaccg tctgtcatgt
     4621 aaggcaacgc cgtttccagc gctcgccagg cggcatcgcg gcctgcacgg tcgccgtcat
     4681 aacagcaaat gacattgttg gtcgcgcgga acaacagttg tatgtgatcg gcggtggttg
     4741 acgtacctaa cgacgcaacg gcgtaattaa tgccgtattg cgccagcgcc accacgtcca
     4801 tatagccttc gaccacaagc agacgattgg gttcagcgtt atcctgctgc gcttcataaa
     4861 gaccgtaaag ctggcggcct ttatggaaaa tgtccgtttc cggcgagttc aggtatttgg
     4921 gggtatcgtt gcccagcacg cgtccgccaa aaccaatcac ccgaccgcgt ttatcacgaa
     4981 tggggaacat cacccgctcg cggaaacgat cgtaactgcg cccctgatcg ttagtgacca
     5041 acatgcccgc atcaatcaat gactggcgat tttctggatt gccgccaaac cgcttcagga
     5101 cgttgtccca gccggggggc gcaaaaccaa tcgcaaagcg agcgataacc tcgtggctta
     5161 atccgcgttt ttccagatac tggcgcgcag acatggcaac aggttgttgt aaagattgtt
     5221 ggtaaaacgt attcagaccg tccatcaact gataaagcgt ttgcctctga tggcgctcga
     5281 tctggctggg gccgctgcct gcttcaaatg gcacttcaag attgtgcatt gctgccagct
     5341 cttcgaccgt ttcgacgaac tcgagcttgt cgtagttcat caggaagtcg atcgcgttgc
     5401 cgtgcgcgcc acatccaaag cagtggtaaa actgtttctc accgttaacg gtgaatgacg
     5461 gggttttctc gttgtggaat ggacaacacg cgtggaaatt cttgccctgc tttttcagct
     5521 tcacacgggc atcgatcaga tcgacgatgt cagtgcgtgc cagcagatca ttaatgaata
     5581 cgcgtgggat tcgtccagcc ataggccccg atttttagca attcataaac gaaaataagc
     5641 cgcgcattcc tttcggaagc acggccttac aactacaact cggtctgatc ggagagcaac
     5701 gctctcgggg aattagtaca gacgagtgcg gcgtgcgttt tcgcgagcca gtttcttcgc
     5761 gtgacgtttc actgcagaag ctttagcgcg cttacgttcg gtagtcggtt tttcatagaa
     5821 ctcacgacga cgaacttccg ccagaacacc tgctttttcg caggaacgct tgaagcgacg
     5881 cagagctacg tcgaacggct cgttttcacg tactttaatt accggcatgt gcctctcacc
     5941 tttgattaat tcggtttgcc gctggcatca acgccagctt atttcaaaat ggtgcggaat
     6001 tttactgcaa ttgctgctgc tttgtaaagc accgcggcct tttttgaaag ggacttttat
     6061 aagggtgagg agtatacacg aggctttctc caggggcgaa caaagtttta catcaacccg
     6121 cattggtcct acactgcgcg gtaataaagc gaggtaaaac aagtcatgcg tgtactgggt
     6181 attgaaactt cctgcgatga aaccggcatc gccatttacg acgatgaaaa aggtttgtta
     6241 gccaaccaat tgtatagtca ggtgaaattg cacgctgact acggcggcgt cgtgcctgaa
     6301 ctggcctccc gcgatcacgt gcgtaaaacc gtaccgttga tccaggaggc gctaaaggag
     6361 tctggtttaa cggcaaaaga cattgatgct gtggcctata ccgcaggccc aggattagtc
     6421 ggcgcactgc tggttggcgc gaccgtgggg cgttctctgg cgtttgcctg ggacgttccg
     6481 gcaatcccgg tacatcatat ggaaggacat ctgttagcgc cgatgctgga agataaccca
     6541 ccggaatttc cgtttgtcgc gctgctggtt tccggcggtc atacgcagtt aatcagcgtg
     6601 actggcattg gtcagtacga gctgctcggc gagtctatcg atgatgctgc tggtgaagcg
     6661 tttgataaaa ccgcgaagct gctggggctg gattatcctg gcggaccgtt actgtctaaa
     6721 atggcggctc agggtactgc cgggcgcttt gttttcccgc gtccgatgac cgaccgtccg
     6781 gggctggatt tcagcttttc tggtctgaaa accttcgcgg caaataccat acgtgacaac
     6841 ggcactgacg atcagacgcg tgccgatatc gcccgcgcct ttgaagatgc agtggtcgat
     6901 acgttgatga ttaagtgtaa gcgagcactg gatcagacgg gctttaagcg actggtcatg
     6961 gcaggcggcg tgagtgctaa ccgcacgttg cgggcgaaac tggcggaaat gatgaaaaaa
     7021 cgccgtggcg aagtgttcta cgcgcgtccg gaattttgta cggataacgg tgcgatgatc
     7081 gcctatgccg gaatggtgcg gtttaaagca ggcgcgacgg cggatctcgg cgtgagtgtg
     7141 cgtccgcgct ggccactggc agagttaccg gccgcgtaaa actattgggt gccggagagc
     7201 aatttccggc accgtcctca cttaaagcaa caccacgggc atccacagta agccggtaat
     7261 caccagcaat acgaggaata tcagcccaaa aatcgccccc agtcgccagt aatccgccgt
     7321 tggcagataa ccactgccgt agtaaatcgg actgggtccg gtggcgtatg gtgtaagaat
     7381 gctccccaga ccaattgccg caccaaccat caggcagaac accggcagcg ggatttccgg
     7441 catcgccagc gcggcggcaa tcatcatcgg tgcgagagcg gaggtatacg ctgtggcgct
     7501 ggcaaaaaag tagcgcagca gataaaagac cacaatcaac gccaccatca ctatcgttgg
     7561 cgaataaccg cttaagctgc ctgctaacag tttgccaaac cagctaataa aaccggtgtt
     7621 gttgagtccg gtagcgaggg tgataagcga ggccagccag aagaaaacgt tccacgccgc
     7681 tttattactg acaatgtcgt cccaactgat aatgcgcaga agcagcatca gcgccaccac
     7741 gctgtaaccg accatcgcgg catcgatata atcaccgcca aaaatccaca acaccagtgc
     7801 gcctaccatc agccccagca tccgtttttc acgcgaacag agcgggccca ttgcctgcag
     7861 ttccgtctct gcccagcgcg gcacctgatc gccagacttc agtaccggcg ggtacagcac
     7921 gtaagccagc cagggaacca gcaaaaccag taaaatgctg agcggcaaca tcccgaggaa
     7981 ccagtcgccc cagcttagca tggcgtgaga tgcgcttttc atcagtccaa ttaacagcaa
     8041 atttggagcc atcgccgtca ggaaaatggc gctggtcacg cagtcggcaa caatccccat
     8101 ccacatgatg tacgagccaa tgctgcgcga actgctgtcg tttggttgtg attgatagag
     8161 cggtggcagg ttacggatga tgggatagat aatccccgca ccacgcgccg agttggacgg
     8221 tgttacaggc gcgagtatca gctcggagaa catcactgca tagccgagaa acagcgtgcg
     8281 atgccccatt tttttcacca gaatcagcgc gatgcgacgt ccaagcccgg ttttttcata
     8341 gcccgtacca aacataaagg cggcgaaaat aagccagata actgaattag aaaaaccaga
     8401 aaccgcccag gagagggatt tggcggtgaa cttaaaacct ggctgggcga gctgctccgg
     8461 gctaaacagc agccacggcg aaagaatggc gatgatggat atacccacca tcgccaccac
     8521 ggcacccggc acgggttcga ggatcagtcc aacgatcacg ccagtaaaaa cggcaaagta
     8581 gagccaggta tgactctcca gccctgcggg aagcggaagt agagcaataa tggcgatgac
     8641 cgccagcggc gcaaggtatc gccaccattc agtggaaggt ttcatcgtgt tatgcctgcg
     8701 aaaaaggaga ggggcgagcc tctccgtgag ggttatttga tgtagtggac gtgctcgcag
     8761 atctcttcca cgatgggatc gcggcgttcg gcgaacagct ttttattttc ggctatcagg
     8821 ttgttgccgt gggtatctat agagacaatc agcgggccaa actctttgac ccggcagacc
     8881 cacagcgact ctggcatccc gagttctgtc cagtgcactt cttcaatctc ttccacttga
     8941 gttgccgcca gtaccgcaca gccagccggg aaaatcacat gcagcgcctt gaatttctgg
     9001 cagccttctt cggtcagcgg ccccattccg cctttgccga ccaccagttt cacgccggtc
     9061 tgctcgataa attcacgttc aaaactttcc atacgcatac tggttgtcgg gccgacggat
     9121 accatctccc atttgtcgcc gtttttgcgc acgatggggc cagcgtggaa aatcgctttg
     9181 ccgttgagat cgtaagggat cggacgtttc agttcgatca agcgacggtg acaaacgtca
     9241 cggcaggtca ccagcgtacc ggtcaggtag atcacatcgc cgacgcgaat atcttgcaga
     9301 tcttcagctt tgatcggggt tgtcaggatc tttttcataa cgcgctccgg gtgtgagaca
     9361 gattttcaaa ggtgagatcg gcatgaacca gcagtgtgcc gcgacgatgc gcccagcagc
     9421 cggtagaaac agcaacgccg atggttgacg gatggcgagc ggcagattcg atatgcacgc
     9481 ccatcactga actgttgcca gtcagccctt gtggaccaat ccccaaacgg ttgagtcctt
     9541 cttccaggcg tagctccagc tctgccgctt tcggattggg gtggcggctg ccaatcgggc
     9601 gcaaaatggc ttttcgcgag agcactgcgg cggtttccac cgaggtagcg atgccaacgc
     9661 ccaccagtac cggcggacag gcgtttacgg cgagggtgga gatattttcg aagacgaatt
     9721 tcactacgcc ttcgtagcct tctgacggca ttaacacttt cgatcggcca ggtagcgtgc
     9781 agccgccgcc tgccatgtaa acttcgattt ccgcatcgtc accgtcgggg acgatatccc
     9841 aggtgaccca cggtacgccg ctgccggtat ttttgccggt gtttacttcg tcaaaaattt
     9901 ctaccgcatt gtgacgcagt ggcgctttga cggttgcctc ttccacggct tgtttgagta
     9961 tgctttgcag ctcgccaagc agtgggaaac gggaaccgac cttaacaaaa aacatgattt
    10021 cgccggtgtc ctgacaggca ggacgattca ggtcgatcgc tttttgcatg ttatcgaaca
    10081 tcgtgtggta gatgattttt cccatcgacg atgtttcggc atcctttagc tgttttagtt
    10141 tatccaccac gtcatcaggc attcgggtag aaatcatggc ggtaaagtta gcgacaatct
    10201 ccgtcaactt attcactgcc tgttgcttat tactttcgct catcatggca actccagttt
    10261 atgtatttgt ctggaaaaat tcaccgccac aaactaccac taaaaatgtg gttttatatt
    10321 ttttgccacc gtgaatatgt aattaacagt tcgtgaatca acgcgaaaat aagaacgaat
    10381 attgcgccag agcagggaat ggtaatgttt tgcgaaatac actgcgttta tcaaaactgc
    10441 ggatatttaa caatgctaaa tagctggcct ttagccaaag acttgcaggt actggtggaa
    10501 atcgtccata gcgggagttt tagtgctgca gcggcgacgc ttgggcaaac gcctgcattt
    10561 gtcaccaagc ggatccaaat ccttgaaaat acgctggcaa caacgctgct gaaccgctcc
    10621 gctcgcggcg tggcgctgac tgaaagtggc caacgttgtt acgaacatgc gctggaaatc
    10681 ctcacccaat atcagcgact ggtcgatgac gtcacgcaaa tcaaaacgcg cccggagggg
    10741 atgatccgca ttggttgcag ttttggtttt gggcgcagcc atattgcgcc agctattacc
    10801 gaactgatgc gcaattatcc tgagttacag gtgcattttg aattgttcga tcgacaaatt
    10861 gatttagtgc aggataatat tgatctggat attcgtatta atgacgcaat tcctgattat
    10921 tatattgcgc atcttttgac aaaaaataaa agaatattat gcgcagcacc tgagtatctg
    10981 caaaaatatc cgcagccaca atccttacag gaattaagtc gtcatgactg cctggtgacc
    11041 aaagagcgcg atatgaccca cggaatatgg gagttgggga acggtcagga gaaaaaatcg
    11101 gtgaaggtaa gcggacatct ttcctccaat agcggcgaga ttgtgttgca atgggcgctg
    11161 gagggcaaag ggattatgct gcgttccgag tgggatgtgt tgccgtttct ggagagtggc
    11221 aaactggtgc gagtattgcc ggagtatgca caaagcgcca atatctgggc tgtttaccgg
    11281 gagccgctct atcgcagcat gaaattacgt gtctgtgtgg aatttctggc ggcatggtgc
    11341 cagcaacggc tgggcaagcc cgacgaaggc tatcaggtca tgtagatcca ccagaaatca
    11401 ctcgggatcc ttttcgcgct ttcttttgaa tttcgtccag atttttgtct cctggcgacg
    11461 ccacagacgc tggatgttgt catgatgacg cagcaggatc aggcaagaga gcatcgaaac
    11521 cgggaaggtg aattgtggct taaaccacca gacataaaac ggagcaatca gtgcactgac
    11581 aatcgctccc agcgacgagt aaccactcaa tagcacggtc agtaaccagg ttcccgccat
    11641 tactccagtg agatcccagc caattggtgc gatggcacca aaagcggtag caacgccttt
    11701 tcctccttta aaaccgaaga aaacgggcca gatgtgtcca agacaggcgg cgatggcaat
    11761 taagcctagc cagaaggggc tgacacctaa ttcatacgcg ccccagacgg gcaacattcc
    11821 tttcagaacg tcgaaaatca gtactgctac ggctgctccc ttgccaccga tacgtaacac
    11881 attggttgcg cctggattgc cggagccgct ggttcgcgga tcgggtaacc cacacaagcg
    11941 gcaaaccaga atggcactgg aaatggagcc gcagaggtac gcgatgagga tcattccagg
    12001 cgcgattgca ctcataagct gttccgtttt gaaaattcgt gttcaacgat gaatctgtgg
    12061 ataatacgca catttcgccg gaagtggtat ccggttagcc aaaaagcagg caggacgtga
    12121 tggatattgt atttatagag caactttcgg taatcaccac tattggtgtt tacgactggg
    12181 aacagaccat cgaacagaag ttagtgttcg atatcgaaat ggcgtgggat aaccgtaaag
    12241 cggcgaaaag tgatgatgtg gcggattgcc tcagttacgc tgacattgca gaaacggtgg
    12301 tcagccacgt cgagggggcg cgttttgcgc tggtggaacg cgtggctgaa gaggtggcgg
    12361 aactgctgtt agcacgcttc aactcgccgt gggtgcgtat caaactcagc aagccaggcg
    12421 cagtggcgcg ggcggcgaat gttggcgtaa tcattgagcg tggcaataat ctgaaagaaa
    12481 ataattaatt ttacagctgt taaaccaaac gattataacc tggtcatact gcagtagttc
    12541 ggacaagcgt tacattttta taatttaggg gtttattgat gagcgatatg cactcgctgc
    12601 tgatagcggc aatattgggt gtggtcgaag gattgacaga atttctgccg gtatccagca
    12661 cgggccatat gattattgtc ggtcacttgt tggggtttga gggcgacacg gcgaaaacct
    12721 ttgaagtcgt gatccagtta ggatcaattc tggcggtagt agtgatgttc tggcggcgtc
    12781 tgtttggcct gattggcatc cactttggcc gcccgttgca gcacgaagga gaaaacaaag
    12841 gtcgtttaac gctgatccac attttgctgg ggatgattcc ggcggtggta ctggggctgt
    12901 tgttccacga cacgattaaa tcattgttta acccgataaa tgtcatgtat gcactggtcg
    12961 ttggcggtct gttgctgatt gctgccgaat gcctgaagcc gaaagagccg cgtgcgccgg
    13021 gtcttgatga tatgacctat cgtcaggcgt ttatgattgg ctgtttccag tgtctggcgc
    13081 tgtggccggg tttctcccgt tccggggcga ccatttcagg tgggatgctg atgggggtga
    13141 gccgttacgc tgcttccgag ttctcgttcc tgctggcggt gccgatgatg atgggcgcaa
    13201 cggcgctcga tctctacaaa agctggggct tcctgactac cggcgatatc ccgatgtttg
    13261 ccgttgggtt tatcaccgct tttgtggtgg cgctgatagc gattaaaacc ttcctgcaat
    13321 tgattaagcg catttcgttt atcccgttcg ccatttatcg ctttattgtg gcggctgcgg
    13381 tgtacgtcgt gttcttttga ttgagagata gtgtcggata tgacgcttgt gcgtcttatc
    13441 cgacctaagg gatttctgca atatactgaa tttaaaaagt taatcaggct ttgggcaacg
    13501 ttgttccttc cagctggcta ccgccgcaat ccgccgtcgg gtcagctcct cacgaatctc
    13561 cacaccttta aatcctgctt caacgacggc ttttgtcggc actgactgcg ccacttccca
    13621 ggcttcgcgc aaccagcggc cttgcgggta gtccgctgat tcaaaaccgg ttctgccgcg
    13681 cacgtcagcc tcgctggtca gcgccagttg ctcgacacgc tgcggtttac gccaggcgtc
    13741 aatggaatca aacaatttaa caatggtttt cgggttcagc attggaaagg tgtggatgag
    13801 atcgtgaaac tcagctacca gtctggctaa atcgcgaatt tcatttggca cgcgcagacg
    13861 ctggcatagt tgttccacta acttcacacc cgccgggcca tgaccatgat gacgtggcca
    13921 aagttctggc ggcgtcagtc ctttaccgag atcgtggcat aacgttgcga aacgaacatc
    13981 gacctgcgga ctcagcatcg ccgccattga gagcgtcatt aaggtatgaa tacccgtgtc
    14041 gatttccggg tgccacctgg caggggccgg aacgccaaac agggagtcaa tttccgggaa
    14101 taaaacgcgc agtgcgccgc aatcgcgcag tacctggaag aacacctggg gattgcgggt
    14161 ggtgagggcg ctttccgtct ctttccatac ccgttcaggc gtcaggtgtt ccagttcacc
    14221 cgcatgggtc atctcgcgca tcagcgccag agtttcatcg gcaatacgga aactgaggtg
    14281 ggcataacgc gcagcaaaac gcgctacgcg caacacgcgt aacggatctt cgccaaaagc
    14341 gggggaaaca tggcgcaaca gacgattttg cagatcgccc agaccgttgt acgggtcgat
    14401 aatctcgccg ttatcgtcct gggccagcgc attaatggtc agatcgcgac gcttaagatc
    14461 atcttccagc gagacatccg gtgcggcata gcaggtaaaa ccggtgtaac cggaaccaga
    14521 tttccgttcg gtacgtgcca gtgcatactc ttcatgcgtt tgcggatgca gaaacacagg
    14581 aaaatcgcgg cctacctgct ggtagcccgc gtcgagcatc tcctgtggcg tactgccgac
    14641 caccacccaa tctctgtctt tgaccggtag ccctaacaat gcatcccgaa cagcaccacc
    14701 gaccagataa atcttcacgc catcatccct tcgtcaaata cacaattcct aataataaga
    14761 cagtgcaacg aagaaggcga tttagttcat ccagcgatct ttgcgtttgc ggcttgggat
    14821 caggtgcggc agtaccagac caagcagcaa gccaagcccc agcacgccac cgccatacat
    14881 aaaccactgc atgatgatgg tgcgctgttt gtcatccaac tgtacgctgg cggcatcgac
    14941 ctttttctgc gcgacaatca gctcgttttt cagtttctga ttttcttctt ttaacctgtt
    15001 gatcacgctg tcactctgcg ccactttttg ctgcatttct gcggtgcgct gattccaggt
    15061 gttatcgata ttggtgagtt tatcggtcag agttttgacc tgattttcca gatctggcac
    15121 acgggagcgc aggcttggtt cagtgctaag ttgtttcaac gggatccagg cagtacggcc
    15181 agagctgtct ttcacctggg cataattggt gttggcgtca gtttgtaata aggtcacttc
    15241 ctcgccggcg ttaaccgtgc ccacgaggcg ataatgatct cccggaccgc tacggaccca
    15301 ggtattcagt tcgtcggaaa catagcgcgt ttcttcagcg tgtgagacgg cagtcgcgct
    15361 aagtgcgagt aaagttaatc cgatcaggcg taattttggc atcaggctgt cgttattgtc
    15421 atggaaagtg gaacgatagt agtggcatca gtgtcgctac gcaaagcatt cgacatcaat
    15481 cggaatcctc tgtgtcaggt cttgccactg cttcaaactt ttacgcccgt caaattgccc
    15541 gttgcatggc aatttggcgc aaaatactat ctactgacaa aaaagatcgc attaagttcg
    15601 ccgttcatcg tcacttatgt gacataacca gtaagtaaag gccatggctc aggaaatcga
    15661 attaaagttt attgttaatc acagtgccgt tgaggcgctg cgtgaccatc tcaatacgct
    15721 gggcggcgag caccatgacc ccgtgcagtt gctgaatatt tactacgaaa cgccggataa
    15781 ttggctgcgt gggcacgaca tgggcttgcg tattcgtggc gaaaacggtc gctatgagat
    15841 gaccatgaaa gttgcaggaa gagtgacagg cggcttacac cagcgcccgg aatataacgt
    15901 ggcgttgagc gaaccgacgc tcgacctggc gcagttaccg acggaagtct ggccgaacgg
    15961 cgaattgcct accgatctcg cttcccgcgt gcagccgctg tttagcaccg atttttatcg
    16021 cgaaaaatgg ctagtagagg tcgatggcag ccgaattgaa atcgctctcg atcagggtga
    16081 ggtgaaagcg ggtgaatttg ctgaacctat ctgtgagctg gaactggaac tgcttagcgg
    16141 cgacatgcgt gcggtgctga aactggcgaa ccaactggta tcgcaaaccg gattacgcca
    16201 gggcagcctg agcaaagcag cgcgtggcta tcatctggcg cagggcaatc cggcgcgtga
    16261 aatcaaaccg accaccattt tgcatgttgc ggcaaaagct gatgtggaac aggggctgga
    16321 agcggcgctc gagctggcgt tagcgcaatg gcagtatcat gaagaactgt gggtacgcgg
    16381 caacgatgcg gcgaaagagc aggtgctggc agccattggc ctggttcgtc atacactgat
    16441 gctgttcggt ggcattgtgc cgcgtaaagc gagcactcac ttacgtgatc tgctgactca
    16501 atgcgaggcg accattgctt ctgcggtttc tgccgtaacg gcggtctact ctaccgaaac
    16561 ggcaatggcg aagctggcgt tgaccgaatg gctggtaagc aaagcctggc agccgttttt
    16621 agatgccaaa gcgcagagta aaatgagcga ttccttcaaa cgctttgccg atatccatct
    16681 ttcccgccat gccgctgaac tgaaaagcgt tttctgccag ccgttaggcg atcgctaccg
    16741 tgaccaattg ccacgcctga cgcgtgatat tgactcaata ctgttgctgg cgggttacta
    16801 cgatcctgtc gtcgcgcaag actggctgga gaactggcag gggctgcgtc acgctattgc
    16861 gaccgggcaa cgcattgaaa ttgaacattt ccgtaatgag gcaaacaatc aggaaccgtt
    16921 ctggttgcac agcggaaaac gttaatcagg caaggataaa atcgcttatg aagccgctct
    16981 cttcaccgtt acagcagtac tggcagaccg ttgttgagcg gctgccagag cctttagccg
    17041 aggaatcact tagcgcacag gcgaagtcag tacttacttt cagtgatttt gtgcaggaca
    17101 gcgtgattgc gcatccagag tggctgacgg aactggaaag ccaaccgccg caggccgacg
    17161 aatggcagca ttacgctgca tggttgcagg aggcgctcag taatgtgagt gacgaggctg
    17221 ggttaatgcg cgagctgcgg ctattccggc ggcgcattat ggtgcgcatc gcctgggcgc
    17281 aaacgctggc actggttact gaagagagca tattgcagca gctcagccat ctggcggaga
    17341 cgctgattgt tgcggcgcgt gactggctgt atgacgcctg ctgccgcgaa tggggaacgc
    17401 cgtgcaatgc gcagggcgaa gcgcaaccgc tgctgatttt aggcatgggt aagctgggcg
    17461 gcggggaatt gaatttctcc tcagatatcg atctgatttt tgcctggccg gaacatggtt
    17521 gtacccaggg cggacgccgg gaactggata acgcgcagtt ttttacccgc atggggcagc
    17581 ggctgattaa agtactggat caaccaacgc aggatggctt cgtctatcgc gtggatatgc
    17641 ggctgcgtcc gtttggggaa agtggcccgc tggtgctgag ctttgcggcg ctggaagatt
    17701 attaccagga gcaggggcgc gactgggagc gttacgcgat ggtcaaggcg cggattatgg
    17761 gggatagcga cggcgtctat gctaacgagt tgcgtgcgtt gctgcgcccg tttgttttcc
    17821 gtcgttacat cgatttcagc gtgattcagt cgctgcgcaa catgaaagga atgattgccc
    17881 gtgaagtgcg tcgacgcggt ttgaccgaca acatcaaact tggcgcaggc ggcattcgcg
    17941 aaattgaatt tatcgtccag gtgttccagc tcattcgcgg cgggcgcgaa ccgtcgctgc
    18001 aatcgcgctc tttactgcca acgctcagcg ccattgccgc gctgcatctg ctttctgaaa
    18061 acgatgctga acaattgcga gtggcgtatc tgttcctgcg gcgtctggaa aacctgctgc
    18121 aaagcattaa cgacgaacaa acccagacgc ttccttctga tgagcttaac cgtgcgcggc
    18181 tggcgtgggc gatggacttt gctgactggc cgcaattgac cggggtgctg accgcacata
    18241 tggccaatgt gcgccgggtg tttaatgaat tgattggcga cgacgaaagt gaaactcagg
    18301 aagagtcgct gtcggaacag tggcgtgagc tgtggcagga tgcgttgcag gaagatgaca
    18361 ccacgccagt gctggcgcat cttagcgagg atgatcgcaa acaggtgcta atgctgattg
    18421 ctgatttccg caaagagctg gataagcgca ccatcgggcc gcgaggacgt caggtgctcg
    18481 accatctgat gccacatctg ctaagtgatg tctgtgcgcg tgaagacgct gctgtcacgc
    18541 tgtcgcgcat taccgccttg ctggtgggga ttgttactcg caccacttat cttgagctgc
    18601 ttagtgaatt ccctgcggcg cttaagcatt tgatttctct gtgtgctgca tcgccgatga
    18661 ttgccagcca gctagcgcgt tatccattat tgctggatga attgctcgat ccaaataccc
    18721 tctaccaacc aacggcgacg gatgcctacc gtgatgaact gcgccagtat ttgctgcgcg
    18781 tgccggaaga tgacgaagaa caacagcttg aggcgctgcg tcagttcaaa caggcgcagc
    18841 tgttacgcat tgcggcagcg gatatcgccg gtacgctacc ggtaatgaaa gtgagcgatc
    18901 acttaacctg gctggcagaa gccatgattg atgccgtcgt tcagcaggcg tgggttcaaa
    18961 tggttgcccg ctacggtaag ccaaatcacc tgaacgaacg cgaagggcgt ggttttgcgg
    19021 tggtcggcta cggcaagctg ggtggctggg agttaggcta cagttccgat cttgacctga
    19081 tcttcctcca tgattgccca atggatgcga tgactgacgg cgagcgggaa atcgacgggc
    19141 gacagtttta tctgcgtctg gcgcaacgca ttatgcatct gttcagtacg cgaacctctt
    19201 ccggcatttt gtatgaagtg gatgcacgac tgcgtccgtc cggggcggcg ggaatgctgg
    19261 tgacatccgc agaagcattt gctgattatc agaaaaacga ggcctggacg tgggagcatc
    19321 aggcgctggt gcgtgcgcgt gtagtgtacg gcgatccgca gctcaccgcg cactttgacg
    19381 cagtacgccg ggagattatg acgctgccgc gcgaaggcaa aaccctgcaa acagaagtgc
    19441 gggaaatgcg cgagaaaatg cgtgcccatc tcggcaataa acatcgcgat cgctttgata
    19501 tcaaagccga tgaaggcgga attaccgata tcgaatttat tactcaatat ctggtgttgc
    19561 gctacgctca cgaaaaaccg aagttaacgc gctggtcaga caacgtgcgc attctggaac
    19621 tactggcgca aaacgacatt atggaagagc aggaagcgat ggcgctgacc cgtgcttaca
    19681 cgacgctgcg cgatgaactt catcatctgg cattacagga attgccgggc catgtgtcgg
    19741 aggattgctt caccgcagag cgtgaactgg tgcgggcaag ctggcagaag tggctggtgg
    19801 aagaatgaag tatggtatta tcgcgcgcaa attttgaatc tctcaggaga caggaatgaa
    19861 agtaacgctg ccagagtttg aacgtgcagg agtgatggtg gttggtgatg tgatgctgga
    19921 tcgttactgg tacggtccca ccagccgtat ctcgccggaa gcgccggtac ccgtggttaa
    19981 agtgaatacc atcgaagaac gtccgggcgg cgcggctaac gtggcgatga atatcgcttc
    20041 tctcggtgct aatgcacgcc tggtcgggtt gacgggcatt gacgatgcgg cgcgcgcgct
    20101 cagtaaatct ctggccgacg tcaacgttaa atgcgacttt gtttctgtac cgacgcatcc
    20161 gactatcacc aaattacggg tactttcccg taaccaacag ctgatccgtc tggatttcga
    20221 agaaggtttc gaaggggttg atccgcagcc gctgcacgag cggattaatc aggcactgag
    20281 ttcgattggc gcgctggtgc tttctgacta cgccaaaggt gcgctggcaa gcgtacagca
    20341 gatgatccaa ctggcgcgta aagcgggtgt cccggtgctg attgatccaa aaggtaccga
    20401 ttttgagcgc taccgcggcg ctacgctgtt aacgccgaat ctctcggaat ttgaagctgt
    20461 tgtcggtaaa tgtaagaccg aagaagagat tgttgagcgc ggcatgaaac tgattgccga
    20521 ttacgaactc tcggctctgt tagtgacccg ttccgaacag ggtatgtcgc tgctgcaacc
    20581 gggtaaagcg ccgctgcata tgccaaccca ggcgcaggaa gtgtatgacg ttaccggtgc
    20641 gggcgacacg gtgattggcg tcctggcggc aacgctggca gcgggtaatt cgctggaaga
    20701 agcctgcttc tttgccaatg cggcggctgg tgtggtggtc ggcaaactgg ggacatccac
    20761 ggtttcgccg atcgagctgg aaaacgcagt acgtggacgt gcagataccg gctttggtgt
    20821 gatgaccgaa gaggaactga agcttgctgt agcggcagcg cgtaaacgcg gtgaaaaagt
    20881 ggtgatgacc aacggtgtct ttgacatcct gcacgccggg cacgtctctt atctggcaaa
    20941 tgcccgtaaa ctgggtgacc gtttgattgt cgctgtgaac agcgatgcct ccaccaaacg
    21001 gctgaaaggg gattcccgcc ccgttaaccc gctcgaacag cgtatgattg tgctgggcgc
    21061 actggaagcg gtcgactggg tggtgtcgtt tgaagaggac acaccgcagc gcttgatcgc
    21121 cgggatcttg ccagatctgc tggtgaaagg cggcgactat aaaccagaag agattgccgg
    21181 gagtaaagaa gtctgggcca acggtggcga agtgctggtg ctcaactttg aagacggttg
    21241 ctcgacgacc aacatcatca agaagatcca actggataaa aaaggctaac tggaaagcgg
    21301 ttcacagatc tcgcttcgta gcaggcaggt ctattgacgc tgttgttttt tcaacctaaa
    21361 gtaaaggaac aagggtaagg gaggatttct cccccctctg atgagttgtt agtaagtcgg
    21421 gaaacttaac agtaacaaca caaccagtat gatgacgaac ttcatcataa ccctttcctt
    21481 ctataaggcc ccttcctcgg gaggggcttt cccgtttcag ccccttgctg actccccgat
    21541 tgttgatgag cgccggaccc cgcgcaacgc tttcccagag gcagttttgc cttccccgag
    21601 cagtcacgca aaatgctgca acagcctgat catttgcacc ttttagcgga acgcagatat
    21661 aaaaaatccc cggctttttg ggccagggat tattcataga agagggttta ctctgctttt
    21721 tcttcgacgg gagttgttga gcttaaggat gaagtcaatg ccgccagtga gccgcctgaa
    21781 acgccaattt cattgagcaa ggagtcaatc agcggtgcct gtgtgcggta agagagtgcg
    21841 gctgataacg cttgttctgc caggtttccg ccccccacat tacccgtgtt tgcatcaccc
    21901 gtagcgccgc cacgattcag accatcgacc tgaataattt tgatgccgtc gatagacttc
    21961 ataggctcaa cggatttctc aattaccgca ggcagcgcct gcaataatgc cagtttgaat
    22021 ttaaggctgg tttgttcatc agaaagtacg ttgatagcat cgttcagcgc acgttgtgct
    22081 tctgcttccg ccagaccctt tttacgtgta gcttcggcta actcaacgat agccgccgcc
    22141 tgcatttctg cggcttcttt ttctgctttc gcccgcacgg tcagttcaac cgctttggtt
    22201 tctgcatcct gcgctgcggc gatcaacgcc acttgtttag cacgatcggc ttcggcagtc
    22261 tggcgagtgg tttctacgtt ttgttgggcg cttactgctt ctgcgagtgc aaggttagca
    22321 cgcgcttctg cctgggactg ttgttccgat ttggcggcaa tagcgatcga tttcgtctgg
    22381 ttagcgattt cggtgacctg ctgctgttcg atctctttaa tgcgaacttc acgttcggcc
    22441 tcaacctttc ttgagcggac ggcctgttcg cggtcgattt ccgtttcctg aatctgtcgt
    22501 tcagccagaa ttcgcgtctg ctctgcttca cgacgacgtt cagcttcaaa agccgcaatt
    22561 ttcgcattct gctcagcggt acgggtttta acctgctgct cctgctcaag cgtcataaac
    22621 gcttcttgtt gttcaatctc caacttgcgc gaaagtgcat cacggttttt ctcacgcact
    22681 gcgacttcca catcctgttc aacttcgtta cgttcgcgac gacggcgctc cgtctcctga
    22741 gtcagtttgg ttaaaccttc ggcgtcaaag gcattgttcg gattgaaatg ttctttcgag
    22801 gtctggttaa agttggtaag tgaaacgctt tccagttcca ggccattttt cgacagatct
    22861 tcagctacgg tattttgtac tccctgcacg aagttctcgc gggtatcctg taactcatgc
    22921 atggtcattt gtgccgctgt tgcacggagg gcatcgacaa atttatcttc caccagcata
    22981 cgtaagtctt caggcgacag ggtgcgttgc cccagcgtct gggcggcggt ggcaatccct
    23041 tctactgaag gttttacccg cacaaagaaa gcaaccacga catcgacgcg catacgatct
    23101 ttcgtaatca ggctatcaat ggttgagcgg ctgacttcca gcttcagggt attcatattg
    23161 atgggaatta tttcatgaaa gatcggcatc acgattgcgc caccgctcat caccactttt
    23221 tgcccactta aaccagtacg aacaaatgct tgctctgccg aagcgcgacg atagagcctg
    23281 gcgaaaataa ttccaataat aaacagaatg catacggcga taatcgcggt aaacatccag
    23341 gagggcacag aattaacgat atcatccatt attaaatttc cttttgatta ataaatcaca
    23401 atatctgggg ccagggatta ttttccgcca aatatcgcgt tgcagaaagt cggcaaataa
    23461 ttaacacttt gtctccttta gtgaaagttt ttccttcttc aggctccagt agcaaataat
    23521 gtatttggcc aaattgatcg gtgagttttc cttcgcaggg gttacctgaa gtggcttgat
    23581 ggccggtaat taatgccatg ctaccaacat attcttcttc tgtaatagct gaactatggt
    23641 cgcgtggaat ccagggtgca acgactttac cggtataatg cactgcaata attgtaaaca
    23701 gtaaactgac aggaaccacg aacaggttgg ggagtggtga ttgccagagc gtaacgcagg
    23761 catgttgcaa caggatgcca ataagaccaa aaaaaccagc gagtaagcag aggacgacga
    23821 gagctggcaa tcttccaata ttgagataat gaagtgcctg gctaatatga cctgtggtta
    23881 tggaatcata atggtcaaga tgtgcatcaa gagcaccgga gagcatgtga ccgcagataa
    23941 gtgcaaaaat ttctaaaagg ccgatcaaga gtacaaagga aatagcgaaa agataagggg
    24001 tgttatagtc ggcgaataaa atcatatgtc actccaatgt ccatttagtg acgc
//
LOCUS       DAPQZD010000065        23923 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPQZD010000065 DAPQZD010000000
VERSION     DAPQZD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.065"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            230..910
                     /locus_tag="RO454_002509"
     CDS             230..910
                     /locus_tag="RO454_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDY0934327.1"
                     /translation="MSMKTLLNVLTIFSLGTLTGCDYIEKASLIDDLAKQQELQKSKI
                     EELEKQQEQYKNKAELFEKQQNNITNSTRILASIVKTVKEKQDEFVFTEFNPLQTQYF
                     ILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNEKPTDAKALA
                     KWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPAE
                     TRQRKNKK"
     gene            954..5297
                     /locus_tag="RO454_002510"
     CDS             954..5297
                     /locus_tag="RO454_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDY0934328.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            5715..7370
                     /gene="lldP"
                     /locus_tag="RO454_002511"
     CDS             5715..7370
                     /gene="lldP"
                     /locus_tag="RO454_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDY0934329.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7370..8146
                     /gene="lldR"
                     /locus_tag="RO454_002512"
     CDS             7370..8146
                     /gene="lldR"
                     /locus_tag="RO454_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDY0934330.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            8143..9333
                     /gene="lldD"
                     /locus_tag="RO454_002513"
     CDS             8143..9333
                     /gene="lldD"
                     /locus_tag="RO454_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDY0934331.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9381..9854
                     /gene="trmL"
                     /locus_tag="RO454_002514"
     CDS             9381..9854
                     /gene="trmL"
                     /locus_tag="RO454_002514"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDY0934332.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(9907..10728)
                     /gene="cysE"
                     /locus_tag="RO454_002515"
     CDS             complement(9907..10728)
                     /gene="cysE"
                     /locus_tag="RO454_002515"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDY0934333.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10808..11827)
                     /gene="gpsA"
                     /locus_tag="RO454_002516"
     CDS             complement(10808..11827)
                     /gene="gpsA"
                     /locus_tag="RO454_002516"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDY0934334.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11827..12294)
                     /gene="secB"
                     /locus_tag="RO454_002517"
     CDS             complement(11827..12294)
                     /gene="secB"
                     /locus_tag="RO454_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDY0934335.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12358..12609)
                     /gene="grxC"
                     /locus_tag="RO454_002518"
     CDS             complement(12358..12609)
                     /gene="grxC"
                     /locus_tag="RO454_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDY0934336.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12750..13181)
                     /locus_tag="RO454_002519"
     CDS             complement(12750..13181)
                     /locus_tag="RO454_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDY0934337.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13425..14969
                     /gene="gpmM"
                     /locus_tag="RO454_002520"
                     /gene_synonym="pgmI"
     CDS             13425..14969
                     /gene="gpmM"
                     /locus_tag="RO454_002520"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDY0934338.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14979..16262
                     /gene="envC"
                     /locus_tag="RO454_002521"
     CDS             14979..16262
                     /gene="envC"
                     /locus_tag="RO454_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDY0934339.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16266..17225
                     /locus_tag="RO454_002522"
     CDS             16266..17225
                     /locus_tag="RO454_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDY0934340.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17212..18246)
                     /gene="waaH"
                     /locus_tag="RO454_002523"
     CDS             complement(17212..18246)
                     /gene="waaH"
                     /locus_tag="RO454_002523"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDY0934341.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18485..19510)
                     /gene="tdh"
                     /locus_tag="RO454_002524"
     CDS             complement(18485..19510)
                     /gene="tdh"
                     /locus_tag="RO454_002524"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDY0934342.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19520..20716)
                     /gene="kbl"
                     /locus_tag="RO454_002525"
     CDS             complement(19520..20716)
                     /gene="kbl"
                     /locus_tag="RO454_002525"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDY0934343.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            20930..21862
                     /gene="rfaD"
                     /locus_tag="RO454_002526"
     CDS             20930..21862
                     /gene="rfaD"
                     /locus_tag="RO454_002526"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDY0934344.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            21872..22918
                     /gene="rfaF"
                     /locus_tag="RO454_002527"
     CDS             21872..22918
                     /gene="rfaF"
                     /locus_tag="RO454_002527"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDY0934345.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            22922..23881
                     /gene="rfaC"
                     /locus_tag="RO454_002528"
     CDS             22922..23881
                     /gene="rfaC"
                     /locus_tag="RO454_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDY0934346.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
BASE COUNT         6014 a         6185 c         6168 g         5556 t
ORIGIN      
        1 gacactaaac atttgacatt gattgatacc gctcattaaa attcgcgcga ctcataacat
       61 tcgctacaaa ctgaaatcag ttgtcattta ataaaacgtc cgtaatacca attatttcaa
      121 taaatctctt ataagtttta ttgcgaatac atatataaaa aacattcatc agaagaatga
      181 atatggcgta attatcaaca tattggggtt atgaaaagga attcatccta tgagcatgaa
      241 aacactttta aacgtgttaa caatattttc actaggtaca ttaacaggct gtgattatat
      301 tgaaaaagcg agtctgatcg atgatcttgc caaacagcaa gagttgcaaa aaagtaaaat
      361 agaggagctt gagaaacaac aagaacaata taaaaataaa gcagaactgt ttgaaaaaca
      421 acaaaataat atcacaaaca gcaccagaat acttgccagc attgtaaaga cagtcaaaga
      481 aaaacaggac gaatttgtct tcaccgaatt taacccgcta caaacccaat acttcatttt
      541 aaataacggc tcggtcggtt tggcaggccg ggtgctggct attgatgcgg ttgaaaatgg
      601 cagtgtcatt cgtatttcgc tggttaactt attaagcgtt cccgtatcaa atattggctt
      661 tcatgcgaca tgggggaatg agaaacccac cgatgccaaa gctctggcga aatggcaaca
      721 attactattc aacaccaccc tgaattccac tctgcaatta atgcctggtc aatggcaaga
      781 cattaatctg acattaaaag gcgtctcgcc taataacctg aagtatctga agttgtccat
      841 caatatggct aatcttcagt tcgacactgt tcaacctgct gaaacccggc agcggaaaaa
      901 caaaaaataa atgcagcttc tgcgcttata tcaaggaata gagagcatca ataatgaaca
      961 aaatatttaa agttatctgg aatccggcga caggcagtta caacgtcgcc agcgaaacgg
     1021 cgaagagccg tggcaaaaag agcgggcgca gtaagctgtt aatttctgcc ttggttgcag
     1081 gtgggctgtt atcgtcattt ggggcactgg ctgataacta cgaggggcag ccagtggatt
     1141 atggcaatgg ctcagcaggt gacggctggg ttgctatcgg taaaaattca aaagcaaata
     1201 cctttgtcaa tggctctggt gcgagcactg cgctaggtta tgacgcgata gctgaaggtg
     1261 agtacagctc agctataggc tcacgaactc atgcgattgg tggtgcatca atggcctttg
     1321 gggttagtgc acaagcaata ggtgacagaa gtgtcgcgct gggtgcatct tcagtagcaa
     1381 ctggcggtcg ttcgatggct cttggtcgtt acgcaaagac gaatggtttt acatctcttg
     1441 ctattgggga caccgccctt gccgatggtg aaaaaactat tgcgttagga aatacggctc
     1501 aagcttacga aattatgagc atcgccctcg gtgatcatgc taatgcctca aaagaatatt
     1561 caatggcgct gggagcaagt agcgttgcat ctgcggctaa cgccattgcc gtaggccgcg
     1621 acagtgctgc taacggcaca gacagccttg ccttaggcag actatctctc gccagcgcag
     1681 caaacgcgat tgctatgggt gcagagagcg aagctgctga aaatgcgacg gcgattggct
     1741 ttaacgcgga tgcgatcggg aagtccagcc tggcgctggg agacaacgcc agcgctggtg
     1801 aaaccaactc catcgctata ggtcaaggca gcgaggcaag caaacttgat tcaattgcgc
     1861 tgggaagtaa cagcaggtca gcgggtgaga acgccattgc actgggcaac aacagtaatg
     1921 cggggggcaa aaacagcctt gctttcggtt ttaacaccac cgcaaatggt gataacgcag
     1981 tcgccattgg tgcaaattct tccgcaggtg ctgacaatac cgtgtctgta ggtagctcta
     2041 gtttaaagcg caaaattgtc aatatgggta atggtgacat caataatgtc agctccgatg
     2101 ccatcaatgg atcacaactt tatgccatca gtaagtctgt atccgacagg cttggaggat
     2161 atcatgatga tcctgataat gttataaact ctgacgggac gcttaaagct cccacatatt
     2221 atctacaaag tggccaatac aataacgtgg gagaggctct ccagagcatc gacaataaca
     2281 cgttgcactg ggacagcaaa agtaataaat acagtgccag ccatactgtc tttaatgcaa
     2341 atggctcagt taaaagcacc tcggcgaaaa acatcattac cgacgtcgct gatggcacga
     2401 tttcagccac cagttccgac gccgttaacg gttcgcagtt gtataatctg aaacaagatg
     2461 ccttgctatg ggatggcact gcgtttagcg ccaaacatgg caccagcaat actaacagca
     2521 aaatcaccaa cgttgcggat ggtgctgttt cagcctccag taaagatgcc gttaacggtt
     2581 cgcagttgta tgatctgaaa caagatgccc tgctatggga tggcactgcg tttagcgcca
     2641 aacatggcac cagcaatact gacagcaaaa tcaccaacgt actggcaggc acagtatctt
     2701 ccaccagcac cgatgccatt aatggtacac aacttcaaag cactgcaagc accattacgt
     2761 cctatttggg cggcggcgcg gcaatgagta atgacggcgt atttaccggt cctcaatata
     2821 atattggtgg taagaattac accaatgttg gcgcagctct taccgctctg gacaatgacg
     2881 ccctgttgtg ggatgcaacc gctaattcaa atactggcgc attcagtgct gcacacggaa
     2941 cactcaaaac cgccagcaag attaccaacg ttgcaaatgg tgacatatta gccaacagtt
     3001 atgatgctat taatggctca caactttata gtttagctga ttcatttacg tcataccttg
     3061 gcggtggtgc tgatatcaac aatgcgggtg ttctcatagc cccgacctat actatcggtt
     3121 cgaaaacgta caacaacgta ggtgatgccc ttaacgcgat taactcatca ttcagcacct
     3181 caatcagtga tgccctgctg tgggattcta cagcgaacaa attcagcgcc aaacacggtg
     3241 gtaccaatag cattattact aacgttgcag acggtacaat ttcctctacc agcagcgatg
     3301 ctgtaaatgg ctcgcaactc tatgacacga gcaagtacat tgctgatgcc ctgggtggtg
     3361 acgcagaagt taacgctgac ggcaccatga ctgcaccgac ttactctatc gctaatgaag
     3421 attaccacag cgtaggtgaa gccctggatg cgctcgatga taacgcgctg ctgtgggatg
     3481 aagacgcagg tgcctacaac gccagccatg atggcaatgc cagcaaaatt accaacgttg
     3541 cggcaggtga tctctccaca accagtaccg atgctgttaa cggttcccag ttaaacgcaa
     3601 ccaatattct ggttacgcaa aatagccaaa tgattaacca gcttgccggt aacaccagcg
     3661 aaacctacat cgaggaaaac ggtgcgggta ttaactatgt acgtaccaac gacaacggct
     3721 tagcgttcaa cgatgccagc gcctcaggta ttggtgctac ggctgtgggt tataactctg
     3781 ttgccgaagg tgatagcagc gtggccattg gtcaaggtag cagcagctcc gttgataccg
     3841 gcattgcctt gggtagcagc tccgtttcca gtcgcgtgat tgttaaaggt tctcgtaaca
     3901 ctagtgtaac ggaaaatggc gtagttatcg gttatgacac cactgacggt gagttgctcg
     3961 gtgcattgtc aattggtgat gacggtaaat accgtcaaat catcaacgtc gccgatggtt
     4021 ccgaagccca tgacgccgtt acggttcgcc agctgcaaaa tgccattggt gcagtcgcta
     4081 ccacgccgac taaatacttc cacgctaact caacggctga agactcactg gcagtcggtg
     4141 aagactcgct ggcaatgggc gcgaaaacca tcgttaatgg taatgcgggt attggtatcg
     4201 gcctgaacac gctggttctg gctgatgcga tcaacggtat tgctatcggt tctaacgcac
     4261 gcgcaaatca tgccgacagc attgcaatgg gtaatggttc tcagactacc cgtggtgcgc
     4321 agaccaacta cactgcctac aacatggacg cgccgcagaa ctccgtgggt gagttctctg
     4381 tcggcagtga agacggtcaa cgtcagatca ccaacgtcgc agcggggtcg gcggataccg
     4441 atgcggtcaa cgtgggtcaa ttgaaagtaa cggacgcgca ggtttcccag aatacccaga
     4501 gcattactaa cctgaacact caggtcacta atctggatac tcgcgtgacc aatatcgaaa
     4561 acggcattgg cgatatcgta accaccggta gcactaagta cttcaagacc aacaccgatg
     4621 gcgcagatgc caacgcgcag ggtaaagaca gtgttgcgat tggttctggt tccattgctg
     4681 ccgctgacaa cagcgtcgcg ctgggcacgg gttccgtagc agacgaagaa aatacgatct
     4741 ctgtgggttc ttctaccaac cagcgccgta tcaccaacgt tgctgccggt gttaatgcca
     4801 ccgatgcggt taacgtttcg caactgaagt cttctgaagc aggcggcgtt cgctacgaca
     4861 ccaaagctga tggctctatc gactacagca acatcactct cggtggtggt aacggcggta
     4921 cgactcgcat cagcaacgtc tccgctggcg tcaacaacaa cgacgcagtg aactatgcgc
     4981 aactgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcgcatg gttgagatgg
     5041 ataacaaact gtccaaaacc gaaagcaagt tgagcggtgg tatcgcttct gcaatggcga
     5101 tgaccggtct gccgcaggct tacacgccgg gtgccagcat ggcctctatt ggtggcggta
     5161 cttacaacgg tgaatcggct gttgctttag gtgtgtcgat ggtgagcgcc aatggtcgtt
     5221 gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg
     5281 ccggtattca gtggtaatga tccattaacg aatgggtaaa cgccacattg cctgatgcgc
     5341 tacgcttatc aggcctacgg ggtgattcag cacattcctg taggtcggat aaggcgttca
     5401 cgccgcatcc ggcaagataa ggcactccgg gtcaacaatc taagggcaat tctctgatga
     5461 ggattgccct tttctttacc agacatctcc caccacaaga attggcccta ccaattcttc
     5521 gcttatctga cctctggttc acaattcccc aattaaaact cacatcaatg ttgccaacac
     5581 gtaacattta gttaaccatt cattgtcatt acccctacac aacacaattg gcagtgccac
     5641 ttttacacaa cgtgtgacaa agagatgggc aacagactca ttacacggcg tgtgtggact
     5701 gcaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg aatatctggc
     5761 tttccagcct gatagcatcg cttcccatcc tgtttttctt ctttgcgctg attaagctca
     5821 aactgaaagg atacgtcgcc gcctcatgga cggtggcaat cgcccttgcc gtggctttgc
     5881 tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc ttcttctacg
     5941 ggttgtggcc catcgcgtgg atcattattg cagcggtgtt cgtctataag atctcggtga
     6001 aaaccgggca gtttgacatc atccgctcgt ctattctttc gataacccct gaccagcgcc
     6061 tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg
     6121 gcgcaccggt agcaattacc gccgcattgc tggtcggcct gggctttaaa ccgctgtacg
     6181 ccgccgggct gtgcctgatt gttaacaccg cgccagtggc atttggtgcg atgggtattc
     6241 cgattctggt cgccgggcag gtaacaggca tcgacagctt tgagattggt cagatggtgg
     6301 ggcgtcagct gccgtttatg accattatcg tgctgttctg gatcatggcg attatggacg
     6361 gctggcgcgg tatcaaagaa acctggcctg cggtcgtggt tgcgggcggg tcgtttgcca
     6421 tcgctcaata cctcagctct aactttattg ggccggaact gccagacatt atctcttcgc
     6481 tggtatcgct gctctgtctg acactgttcc tcaaacgctg gcaacccgtg cgcgtattcc
     6541 gcttcggtga cttaggggcg tcacaggttg atatgacgct ggctcacaca ggttacacgg
     6601 caggtcaggt gctgcgcgcc tggacaccgt tcctgttcct gaccgccacc gtaacactgt
     6661 ggagtatccc gccgtttaaa gccctgttca cttcaggcgg tgcgctgtat gagtgggtga
     6721 tcaacattcc ggtgccgtac ctcgataaac tggttgcccg tatgccgcca gtggtcagcg
     6781 aagcgacagc gtatgccgcc gtgtttaagt tcgactggtt ctctgccacc ggtaccgcca
     6841 ttctgtttgc tgccctgctc tcgattgtct ggctgaagat gaaaccatct gacgctatca
     6901 gcaccttcgg cagcacgctg aaagaactgg ctctgcctat ctactccatc ggcatggtgc
     6961 tggcgttcgc ctttatctcg aactattccg gtttgtcatc aacactggcg ctggcactgg
     7021 cgcacaccgg tcatgcattc accttcttct cgccgttcct cggctggctt ggggtattcc
     7081 tgaccgggtc ggatacgtca tctaatgctt tgtttgccgc tttgcaggct accgccgcac
     7141 aacaaattgg cgtttctgac ctgttgctgg ttgctgccaa caccaccggt ggtgtcaccg
     7201 gcaaaatgat ctctccgcaa tctatcgcta tcgcctgtgc ggcggtaggc ctggtgggca
     7261 aagagtctga tttgttccgc tttactgtca aacacagcct gatcttcacc tgtatggtgg
     7321 gcgtgatcac cacgcttcag gcctatgtct taacgtggat gattccttaa tgattgtttt
     7381 acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa
     7441 cctggaagcg ggcatgaagt tgcccgctga acgtcaactg gcgatgcaac tcggcgtgtc
     7501 gagaaattca ctgcgcgagg cgctggcgaa actggtaagc gaaggtgtgc tgctcagtcg
     7561 acgtggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt
     7621 ccagccgctg aaaacgctga tggccgatga tccggattac agtttcgata ttctggaagc
     7681 ccgctacgcc attgaagcca gcaccgcatg gcatgcagca atgcgcgcca cacctggcga
     7741 aaaagaaaag attcagcttt gctttgaagc aacgctaagt gaagacccgg atctcgcctc
     7801 acaagcggac gttcgttttc atctggcgat tgccgaagct tcacataaca ttgtgttgct
     7861 gcaaaccatg cgcggtttct tcgatgtcct gcaatcctct gtgaagcata gccgccagcg
     7921 gatgtacctg gtacccccgg ttttttctca gctgaccgaa caacatcagg cggtcattga
     7981 cgccattttt gtcggtgatg ctgacggggc gcgtaaagca atgatggcgc accttagctt
     8041 tgttcacacc accatgaaac gattcgatga agatcaggct cgccatgcac gaattacccg
     8101 cctgcctggt gaccataatg agcattcgag ggagaaaaac gcatgattat ttccgcagcc
     8161 agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgtttca ctatatggat
     8221 gggggggcat attctgaata caccctacgc cgcaacgtgg aagatttgtc agaagtggcg
     8281 ctgcgccagc gtattctgaa aaacatgtct gacttaagcc tggaaacgac gctgtttaat
     8341 gagaaattgt cgatgccggt ggcgctaggt ccggtaggtt tgtgtggcat gtatgcgcga
     8401 cgcggcgaag ttcaggctgc caaagccgcg gatgcgcatg gcattccgtt tactctctcg
     8461 acggtttccg tttgcccgat tgaagaagtc gctccagcta tcaaacgtcc gatgtggttc
     8521 cagctttatg tgctgcgcga tcgcggcttt atgcgtaacg cactggagcg agcaaaagcc
     8581 gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggagc gcgttatcgt
     8641 gatgcgcatt ctgggatgag cggcccgaac gcggcaatgc gccgctattt gcaggcggtg
     8701 acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccgcatga tttaggtaat
     8761 atctcggctt acctcggcaa accgaccgga ctggaagatt acatcggctg gctggggaat
     8821 aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt ctgggatggc
     8881 ccgatggtga tcaaagggat cctcgaccca gaagatgcgc gcgatgcagt acgttttggt
     8941 gctgatggaa ttgtggtttc taaccacggt ggccgccagc tggacggtgt gctctcttct
     9001 gcccgtgcac tgcctgctat tgcagatgcc gtgaaaggtg atatcgccat tctggcagat
     9061 agcggaatac gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta
     9121 ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg tgtagctaac
     9181 ctgctaaatc tgatcgaaaa agagatgaaa gtggcaatga cgctgactgg cgcgaaatcg
     9241 attagcgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt gccggcggca
     9301 ttggctccga tggcgaaagg gaatgcagct taagggttag acgaatatct gctatcctgc
     9361 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
     9421 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
     9481 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
     9541 ttaccgccgt tacgcgtcat catgactatc gcgcgtttct cgaagcagaa aatccccagc
     9601 gtctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
     9661 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
     9721 tgcccgctga acaaaaaatt cgtattccaa tggtgccgga cagccgcagc atgaacctgt
     9781 ccaatgcggt gtcggtcgtg gtgtatgaag cctggcggca gttggggtat ccgggagcgg
     9841 tattgagaga ttagttactg taggccggat agatgattac atcgcatccg gcacgatcac
     9901 aggacatcag atcccatcac catactcaaa cgtatggtta atgccgttga aatgctgatc
     9961 catatccatt gacggtttat cgctatccgg cttgccgaca atgcgtgccg gaacgccagc
    10021 ggcagtagtg tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    10081 gcgcccaact tcaatattgc cgagaatttt cgcgcccgcg ccaatcatca caccttcacg
    10141 aattttcggg tgacggtcgc caccagattt acccgtaccg ccaagcgtca cagattgcag
    10201 aatcgatacg tcgttttcaa tcaccgccgt ttcaccaacg acgatgcctg tcgcgtggtc
    10261 gagcatgata ccgcgaccaa tttttgctgc cgggtgaata tcaacctgga acgtcacaga
    10321 aacctggttt tgcagaaaaa tagccagtgc gcgacgcccc tgattccaca accagtgacc
    10381 gatgcgatac gcctgcaaag catgaaaacc cttcaggtat agcaacgggg ttgagtattt
    10441 atccaccgcc gggtcacggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    10501 ttccgggtcg gcggcgtagg cttcttccac cacttcacgg atagcaatag ccggcataat
    10561 gggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt tttcgtgctt
    10621 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    10681 ttcagcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    10741 acgatgagat aatgaccgac ccgttctgcg cgggccgggt catagtggta acaaaggttc
    10801 cctgggttta gtggctgctg cgctcgtcct tgcgtgcacg acctagtaaa gtcaatgctg
    10861 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    10921 caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    10981 cttgaccaat tttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    11041 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    11101 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atctcagcca
    11161 gcccacgagt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    11221 ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacaccg atgaaatccg
    11281 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgctggagg tcatcggcaa
    11341 aggtctggtc cgtcgaggcc agcgaaatgg ccgtcggtaa acctgccgcc agttctttcg
    11401 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    11461 cgtcctgtaa cagacgtccg gtttctgctt ccagcccttt ggttgcccac accagacgcg
    11521 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    11581 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    11641 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    11701 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    11761 tgatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    11821 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    11881 catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg taccgcggga
    11941 taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    12001 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    12061 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    12121 cacttcgtaa acgtcatctg ccagttgagt agatgccgta tccagatcaa gtttaacttc
    12181 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag aaatatcctt
    12241 ggtataaata cgttggatct ggaaagtcat ttcggtgttg ttttgttctg acatgtgtag
    12301 aaaaccctta agtgttgtcc ttaaaatact gcgcaatgta atgccaacgt tcacacgtta
    12361 tttcagcagg ggatccagtc caccacgtgc atccaatgca tacaagtcat cacagccacc
    12421 aatgtgctgt gcgtcaataa aaatctgtgg aaccgtggtg cgaccgctgc gtttgatcat
    12481 ctcttcacgc ttagcggcat tgccatcgat cggcaactcc tgaaaactca cgcccttgct
    12541 gcttagcagt gcttttgcac gatggcaata cgggcaggtt tctttggtat agatttcaac
    12601 attggccata aattagctcc tgttttttac cctgaaattt catgttgcag ggcggcagca
    12661 aaatggagtg tccccagaag cttatatcaa taagtcgctg ggggcctaat tgcagctaac
    12721 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccaaagg caagttttcg
    12781 ccagcccagc cagcgacgcc ttctttcagt acgaatactt gtgcaaaacc agctttcgtc
    12841 agcgcgtttg caggctcctg gcactgcatg ccagaaccat ctaccacgat aaccggtttg
    12901 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacaggttg
    12961 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    13021 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    13081 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    13141 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    13201 cgtagtgatt aaaaaaccag gaaaggagta tacctgcgca gtgcggcaaa tacagccagc
    13261 gcgttaactg gaatgcaatt ttgcggggcg ctacgaaaaa aaagcgtaaa tctggcatct
    13321 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    13381 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    13441 aacctatggt actggtgatt ctggatggct atggctatcg cgaagaacag caggataacg
    13501 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    13561 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    13621 taggccacgt taacctgggt gcaggccgca tcgtgtatca ggacctgact cgtctggacg
    13681 ttgaaatcaa agatcgcact ttctttgcta atccggtgtt gactggtgcg gtagataaag
    13741 cgaaaaacgc aggcaaagca gtacacatta tgggtctgct ctccgcaggt ggtgtacaca
    13801 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    13861 tctacctaca cgcattcctt gacggtcgcg acactccgcc gcgcagtgct gaatcctcgc
    13921 tgaaaaaatt cgaagagaaa tttgccgcgc tgggtaaagg ccgcgtagcg tccatcattg
    13981 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcttatgacc
    14041 tgctgactct ggcgcaaggt gagttccagg ccgataccgc cgttgccggt ttgcaggctg
    14101 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    14161 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    14221 gcgcacgtga aatcactcgt gcattcgtaa atgccgattt tgacggcttc gcgcgtaaga
    14281 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc tgccgacatc aaaaccgcag
    14341 tggcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    14401 aaactcagct gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    14461 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    14521 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    14581 caatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    14641 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcgctggat cactgcgtgg
    14701 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    14761 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacagca cacaccaacc
    14821 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgaa ggcggcaaac
    14881 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    14941 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    15001 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    15061 ttctgagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    15121 tcaaatccat tcaggctgac atcgccgcga aagagcgcgc ggtacgccaa aagcaacaac
    15181 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    15241 gtaaactgcg cgaaacgcaa aacacgctta atcaactgaa taaacagatt gatgagatga
    15301 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcgc
    15361 aactggatgc cgcgtttcgt cagggtgaac ataccggtat tcagctgatt ctcagcggtg
    15421 aagaaagcca gcgtgggcag cggttacagg cttatttcgg ctatctcaac caggcgcgac
    15481 aagaaaccat tgctcagttg aaacaaacgc gtgaagaagt cgctatgcag cgtgccgaac
    15541 tggaagagaa acaaagcgag caacaaacgc ttttatatga gcagcgcgcc caacaggcga
    15601 agctgaccca ggcgttgagc gagcgtaaaa agacgctggc agggctggag tcttccatcc
    15661 agcaaggtca gcaacagttg agcgagctgc gcgccaacga atcccgcctg cgtaacagca
    15721 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg tgaagcgcgc gaagcccagg
    15781 cggttcgcga ccgccagaaa gaagcgacgc gcaaaggcac cacctacaag ccgaccgaaa
    15841 gcgaaaaatc gctgatgtcc cgtaccggtg gtctgggcgc accgcgcggt caggcattct
    15901 ggccggttcg cgggccaacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    15961 ggaaagggat ggtgattggt gcctctgaag gtactgaagt taaagcgatt gccgacggcc
    16021 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcatggta
    16081 aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt ggttcgcagg
    16141 ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt cggccttcac
    16201 tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg ttgggaagat
    16261 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    16321 cccccgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgcaca
    16381 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    16441 cgcacgccag agagatggcg accaaagcac ataacagcgg tcacgaagtg ttgattcatc
    16501 taccgatggc accactgagt aaacagccgc tggagaaaaa tacgctacgc ccggagatga
    16561 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    16621 ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    16681 tgcaggcgct ggagcgttac aatctttact ttctcgacag cgtaaccatc ggtaataccc
    16741 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    16801 acgattcgca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    16861 gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgctac
    16921 aacagctggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    16981 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    17041 atccgttccg tggcgtgaag ctgtgcaaac cgaagaagcc gctggaacct gtttatgcta
    17101 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    17161 agcatcagtg gcaaggctgg ggcaaacagc ccgaagcggc aaaacttaac gctagcgcaa
    17221 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    17281 gcggaaagac cacagtaacg cctggtaacc caccttcacg ctgcggacat tggtaatcag
    17341 gcgcttatac ataccggaag taaatatctc ggcaatcatc cgttggcggg taagaatatc
    17401 cggctctttg cgcaccgcat ggcaaacacg caatgcttcg taagttattt gctgatgaaa
    17461 ttccggataa atcgtaattt tgtcagcata atttcgattt aatttctcca gcaggcgggt
    17521 aatcttaata tagtgacgtt gataattaag gtttttattc ccttgtctat gcaaccgact
    17581 cactgacgta ttatgcagat aatatttata taatgattgc tcggtatatc gcgcacgcag
    17641 cgcgttaaac ataaattctg ttgtccagac aatatcctga tgatgtaatc cggcaataaa
    17701 tttaatgtta tttttaacaa taacatcgcg gcgataaacc cccatccaga ccacgtgtgt
    17761 ccaacggcgg gaagaaagtc ccatccgcag ccagtccgga ccggttaata caccggttga
    17821 gcgaaggcga tcggtgggga tggattgcca ggtttcaccc gtttcacgaa aacaccaatc
    17881 agcgttgcac tgcgccacgt cgaggtcatc ctctaacgcc atggtcatca gcgtttcgta
    17941 catggtggga tagacctcat cgtcagcatc gacaaaagcg acatattttc ccgttgccac
    18001 ttcaatccca cgattacgcg ccaccgatgc gccagcattc gcctgatgca acaaacgaac
    18061 gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg ttgaaccatc
    18121 gttaataata atgatttcca gagcagtcca tgtttgcgta attaaagatt ccatacaagt
    18181 gcggaaatca tcgcccgcat tatataacgg gataataaca ctaagtttat tggtgctgtt
    18241 catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct ttgtattaag
    18301 aaactattaa gcctgtgaga tattattttt accggtgaga gagtcacttc agggtagaga
    18361 gataatgaga gcagctatat aagagatcag tgcaattacg aactctttat tacactgaca
    18421 ggattttaca tatttaacgg attatcctca ggcaaaaggg ctggaatacc agcccttgtt
    18481 cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata gcgtcaaagc
    18541 ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga tcgaggccag
    18601 actgaatcag cgccgccatc ttgtaccagg tttcaaacat ctcacgaccg taaatacctt
    18661 taatgaacaa gcctttaaag attactttgg tccagtcgat agacatatcg gacggtggaa
    18721 tacccagcat cgcaatacga ccgccgtggt tcatggtgtc gagcatggta cgaaacgctg
    18781 gcggcgcacc ggacatttcc agaccgacat caaagccttc ggtcatgccc agttctgcca
    18841 tcacatcatt aaggttttct ttagcgacgt taaccgcacg ggtgataccc attttgcgtg
    18901 ccagctcaag gcggtattcg ttaacatcag tgatcaccac attgcgtgca ccaacgtgtt
    18961 tcgccaccgc cgcagccata atacctatcg gacctgcacc agagaccagc acatcctcgc
    19021 caaccagatc gaacgacagc gcggtatgca cggcgttacc gaagggatca aaaatggaag
    19081 ccaggtcgtc ggagatattg tctgggattt tgaaggcgtt gaacgccgga atcaccagat
    19141 actcggcgaa gcagcccgga cggttaacac cgacgccgat cgtgttgcgg cacagatggg
    19201 tacgaccgcc acgacagttg cgacagtgac cgcaggtgat atggccttcg ccagaaacgc
    19261 gatcgccaat cttgaagcct ttcacttcct gaccaatacc taccacttca ccgacatatt
    19321 catggcccac gaccatcggc accgggatgg ttttttgcga ccactcatcc cagttataga
    19381 tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga tcgttatgcc
    19441 cgagttccgg tacaggaacg tcggtcatcc agatgccctc ttccgctttc agtttggata
    19501 acgctttcat ctcacaccct caggcgataa cgcccagttg tttaccaata cgcgtgaatg
    19561 cttctactgc acgcgtaatt tgctcagggg tatgcgccgc agacatctgg gtacgaatac
    19621 gcgcctgacc tttaggaacg accggataga agaaaccggt gacgtaaatg ccctcttttt
    19681 gcagttcacg ggcaaatttt tgcgccacta cagcatcgcc aagcatgacc ggaataatgg
    19741 cgtgatcggc acccgccagg gtaaagcccg ccgccgacat ctgctcacgg aactgacgcg
    19801 cgttcgccca cagacggtca cgaagctcac tgcccgcttc gaccatctcc agcactttaa
    19861 tggaagcagc cacaatggca ggtgccagtg agttggagaa caggtaagga cgagaacgct
    19921 gacgcagcca ctcaaccact tctttacgcg ctgcggtgta accaccagaa gccccgccca
    19981 acgctttacc aagcgtaccg gtgataatat cgacccgccc catcacatcg cagtattcat
    20041 gggaaccacg accattttca ccgacaaaac cgaccgcgtg ggagtcgtct accatcacca
    20101 gggcatcgta tttatctgcc agatcgcaaa cgcccttcag gttggcaatc acgccgtcca
    20161 tcgagaacac accatcggtg gcaatcagca catgacgcgc accggcttca cgcgcttctt
    20221 tcagacgagc ttccagctca tgcatatcgt tgttggcata acgatagcgt ttagctttac
    20281 acagacgcac accatcaata atagacgcgt ggttcagtgc gtcggagata atggcgtcct
    20341 ccgcgctcag aagcgtttca aacaggccgc cgttagcatc aaagcaggaa gagtagagaa
    20401 tcgcatcttc catccccagg aatgccgcca gtttttgttc aagctcttta tggctatcct
    20461 gggtgccgca gataaagcgc accgaagcca tcccaaaacc atgagagtcc attcccgctt
    20521 ttgccgccgc aatcagctct ggatgatttg ccagtccgag atagttattg gcgcaaaagt
    20581 taataacgtg gcttccatca gccacagtga tatctgcttg ctgcgcagac gtaataatgc
    20641 gctcttcttt aaacaaccct tccgcccgtg cggtttccag atcgttggtt aactgctgat
    20701 aaaattctcc acgcattgcg attctccaga cagggcaaat tccagcacat attacccaaa
    20761 cttctaggtc gcgacgagat gacgcgttaa cacttctgca aaattcagga taaataacgt
    20821 ccatcaatct cacttttggc gaatggctga agccaacgcc gtgtagtgat atgatagaag
    20881 tattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt
    20941 taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg ataaaggcat
    21001 caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga
    21061 tctggatatc gcggactata tggataagga agacttcctg atccagatta tggctggcga
    21121 agagttcggc gatgtcgaag cgattttcca cgaaggcgcg tgctcttcca ccaccgagtg
    21181 ggacggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc tgcactactg
    21241 tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacttacg gtggacgcac
    21301 ctccgacttt attgaatccc gcgaatatga aaaaccgttg aatgtctacg gttactcaaa
    21361 attcctgttt gatgaatatg ttcgccaaat cctgcctgaa gcgaactcgc agattgttgg
    21421 cttccgttat ttcaacgttt atggacctcg tgaaggccat aaaggcagta tggcgagcgt
    21481 cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt tcgaaggtag
    21541 cgagaacttt aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa acctgtggtt
    21601 cctggaaaat ggcgtttccg gcatcttcaa cctcggcacc ggtcgtgcgg aatccttcca
    21661 ggcagtagca gatgctacgc ttgcttatca caagaaaggc caaatcgaat acattccgtt
    21721 cccggataaa ctgaaaggcc gctaccaggc gttcactcag gcagatctga cgaatctgcg
    21781 cgcggcgggt tacgataaac cgttcaaaac cgttgccgaa ggcgtaacgg aatacatggc
    21841 ctggctgaat cgtgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg
    21901 ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca
    21961 ggcgataatc gacgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga
    22021 agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg
    22081 caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct tacctaactc
    22141 cttcaaatcc gcattagtgc ctttcttcgc aggtattcct catcgcaccg gctggcgcgg
    22201 cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag cctggccgct
    22261 aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta cagcacaaga
    22321 tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa aatcatatac
    22381 ctgtaatcaa ttttcgctct catcagaacg tccgatgatt ggtttttgcc cgggtgcgga
    22441 gtttggtccg gcaaaacgct ggccacacta ccactatgcg gagctggcaa agcagctgat
    22501 tgatgaaggt tatcaggtgg ttctgtttgg ctcggcgaaa gatcatgaag cgggcaatga
    22561 gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac
    22621 acagcttgat caagcggtta tcctgattgc agcctgtaaa gccattgtta ctaacgattc
    22681 tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccgag
    22741 tagcccggac ttcacaccac cgctatccca taaagcacgc gtgatccgcc tgattaccgg
    22801 ctatcacaaa gtgcgtaaag gtgacgcggc ggagggttat caccagagct tgatcgacat
    22861 tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg aagcctgacg
    22921 gatgcgggtt ctgatcgtta aaacatcgtc gatgggcgat gttctccata cgctgcccgc
    22981 actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg tggaagaagg
    23041 gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg tggcaatacg
    23101 tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag cgtttcgtga
    23161 agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc
    23221 gacgctggtg acgcgtctgg cgcatggcgt aaagcatgga atggactggc aaaccgctcg
    23281 cgaaccgtta gccagcctgt tttacaatcg taaacatcat attgcaaaac agcagcacgc
    23341 cgtagaacgc acccgcgaac tgtttgccaa aagtctgggc tatagcaaac cacaaaccca
    23401 gggcgattat gctatcgcac agcatttcct gacgaacctg cctacagatg ctggcgaata
    23461 tgccgtattt cttcatgcaa caacccgaga tgataaacac tggccggaag aacactggcg
    23521 agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc
    23581 gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc
    23641 gaagatgagt ctggaaggcg ttgcccgcgt actggccggg gctaaatttg tagtgtcggt
    23701 ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg
    23761 acctaccgat ccgggattaa ttggtgggta tgggaagaat cagatggttt gtagggctcc
    23821 aagagaaaat ttaattaacc tcaacagtca agcagttttg gaaaagttat catcattata
    23881 aaggtaaaac atgctaacat cctttaaact tcattcattg aaa
//
LOCUS       DAPQZD010000066        23580 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPQZD010000066 DAPQZD010000000
VERSION     DAPQZD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.066"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..191
                     /locus_tag="RO454_002529"
     CDS             <1..191
                     /locus_tag="RO454_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDY0934347.1"
                     /translation="GVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKAARGE"
     gene            196..540
                     /locus_tag="RO454_002530"
     CDS             196..540
                     /locus_tag="RO454_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDY0934348.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKSPPKW"
     gene            complement(506..778)
                     /locus_tag="RO454_002531"
     CDS             complement(506..778)
                     /locus_tag="RO454_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934349.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            884..1417
                     /locus_tag="RO454_002532"
     CDS             884..1417
                     /locus_tag="RO454_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDY0934350.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VFRRDQESALACWGIDR"
     gene            1972..2058
                     /locus_tag="RO454_002533"
     CDS             1972..2058
                     /locus_tag="RO454_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934351.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            2280..2465
                     /locus_tag="RO454_002534"
     CDS             2280..2465
                     /locus_tag="RO454_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HDY0934352.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2462..2554
                     /locus_tag="RO454_002535"
     CDS             2462..2554
                     /locus_tag="RO454_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDY0934353.1"
                     /translation="MMTMQKQLEGAQEYIRTQCTKLAFYYPEDT"
     gene            2551..2766
                     /locus_tag="RO454_002536"
     CDS             2551..2766
                     /locus_tag="RO454_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934354.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVLQPVC"
     gene            complement(2965..3165)
                     /locus_tag="RO454_002537"
     CDS             complement(2965..3165)
                     /locus_tag="RO454_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDY0934355.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3207..3572
                     /locus_tag="RO454_002538"
     CDS             3207..3572
                     /locus_tag="RO454_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDY0934356.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCAECHRQKSAREAASGMPD
                     PTLPEVSGGSGREDDIIGL"
     gene            3863..4426
                     /locus_tag="RO454_002539"
     CDS             3863..4426
                     /locus_tag="RO454_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDY0934357.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNLEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            4423..6084
                     /locus_tag="RO454_002540"
     CDS             4423..6084
                     /locus_tag="RO454_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDY0934358.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTNLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            6148..8085
                     /locus_tag="RO454_002541"
     CDS             6148..8085
                     /locus_tag="RO454_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDY0934359.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            8130..8351
                     /locus_tag="RO454_002542"
     CDS             8130..8351
                     /locus_tag="RO454_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934360.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            8297..10720
                     /locus_tag="RO454_002543"
     CDS             8297..10720
                     /locus_tag="RO454_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0934361.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPML
                     EQMVKEAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQK
                     SFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQR
                     TEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAK
                     RGRDAGGGK"
     gene            10717..11043
                     /locus_tag="RO454_002544"
     CDS             10717..11043
                     /locus_tag="RO454_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDY0934362.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            11053..11403
                     /locus_tag="RO454_002545"
     CDS             11053..11403
                     /locus_tag="RO454_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDY0934363.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            11400..11846
                     /locus_tag="RO454_002546"
     CDS             11400..11846
                     /locus_tag="RO454_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDY0934364.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            11843..12187
                     /locus_tag="RO454_002547"
     CDS             11843..12187
                     /locus_tag="RO454_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDY0934365.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            12254..12970
                     /locus_tag="RO454_002548"
     CDS             12254..12970
                     /locus_tag="RO454_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HDY0934366.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            12985..13359
                     /locus_tag="RO454_002549"
     CDS             12985..13359
                     /locus_tag="RO454_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HDY0934367.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            13455..13664
                     /locus_tag="RO454_002550"
     CDS             13455..13664
                     /locus_tag="RO454_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDY0934368.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            13712..16954
                     /locus_tag="RO454_002551"
     CDS             13712..16954
                     /locus_tag="RO454_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDY0934369.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVARVQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            16947..17288
                     /locus_tag="RO454_002552"
     CDS             16947..17288
                     /locus_tag="RO454_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0934370.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNLTVRVVCREWSVTDNARHS
                     DFSCTIEQVVN"
     gene            17288..17986
                     /locus_tag="RO454_002553"
     CDS             17288..17986
                     /locus_tag="RO454_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDY0934371.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIDGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            17997..18740
                     /locus_tag="RO454_002554"
     CDS             17997..18740
                     /locus_tag="RO454_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0934372.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTSEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            18686..19318
                     /locus_tag="RO454_002555"
     CDS             18686..19318
                     /locus_tag="RO454_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDY0934373.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWY
                     QIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVIGRQG"
     gene            19661..23134
                     /locus_tag="RO454_002556"
     CDS             19661..23134
                     /locus_tag="RO454_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDY0934374.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEI
                     TQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     AGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGITIS"
     gene            23202..>23580
                     /locus_tag="RO454_002557"
     CDS             23202..>23580
                     /locus_tag="RO454_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDY0934375.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT         5349 a         5724 c         7504 g         5003 t
ORIGIN      
        1 caggtgtgtc atacaccacg tcagcggtgg gaacgggcta ctggttcctg cagttgctgg
       61 acagggtttc cccgtctcag tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc
      121 tgctgacata tctgactaac ctgtatttca aaatcagaga ggaccgtcgt aaggcggcac
      181 ggggagagta attcaatgac tcaaaactat gaactgattg tgaaagggat ccgcaatttt
      241 gagaataaag ttacggtaac tttagcgtta cgggacaaaa aacgctttga cggtgaaatt
      301 tttgacctgg acatctcgct ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca
      361 gcagccagaa ggagcatcag acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg
      421 gatgagcagt cgccggaaaa gcgccccgta attttagagg cgcaggatgt gttgataacc
      481 tacagaggaa aactaccggg aataattacg ggttctctga agagtccgcc gaaatggtaa
      541 ttttaccagc atatttttca tccagtaata cagcaagccg cctgaaagag tcttgttgtt
      601 cctgagacca tttgggattg catgattcaa actggattga tgccagcgtt gattgcatct
      661 gttcccttgg aattgagaat gccagatatg agaaggcgac ggtaagggta ttcacgtctt
      721 cccgaagcct ggaaatgctg tcgagcaact cctgtagaga aatggtgtta ttgtccataa
      781 ataatcctca tgattgtatt gacctgttag cagcctgagg caacaggctg gaactgataa
      841 acatatccag ggctcagaaa ccgataaatc ctgataaata tccatgaacg caaaaatcag
      901 atacggcctg tcggctgccg ttctggcgct gattgccgct ggtgcgcctg cgcctgacat
      961 tctcgaccag tttctggatg aaaaggaagg taaccacacc acggcatacc gtgatggcgc
     1021 gggtatctgg accatctgcc gcggtgccat cctggtggat ggcaaacctg tcgttccggg
     1081 catgaagttg tcgaaggaaa aatgcgaccg ggttaacgcc attgagcgtg ataaggcgct
     1141 ggcatgggtg gagaaaaaca tcaaagtgcc attgaccgaa ccccagaaag cggggatcgc
     1201 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
     1261 aattaatgca ggtgatcgca ggggagcatg cgaagcgatt cgctggtgga ttaaggacgg
     1321 tggcagagac tgccgtattc gctcaaataa ctgttacggt caggtattcc gtcgtgacca
     1381 ggagagcgcg ctggcgtgct ggggaatcga cagataagca gaatattttg ctgaaaaatg
     1441 aggttggcca acgcgggcgg ataacacgaa gtcctgtgaa ctggcaaaac ctaagtgaat
     1501 aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt gtttttatgg cagtaagcta
     1561 tgggaggctg ccttgattga ttttagcaaa ctgattaggg agttgcgact catgattagt
     1621 caattaccaa actggaaatt tttgctggtc tggagcatcc cttttttatg ggtagtatcc
     1681 cagttaattg tggcaattaa ggggtagcta tgtcagacaa actcataacg ccggcaaagg
     1741 tcctgtgtgt gattgtcggt atttcatttt cactaatgct ggttgctctt tttctgtccc
     1801 tcgcctgggt gatgttgtct tcgtcggggc tgctggggtg acagtgactg atgacatcag
     1861 cagagcgctg gcttttgcta ttaagtgggt ggctgttggt attgctgtgt ctccgatgct
     1921 gtatgggctg gcaaaactgg tcattgcgct gaaatcgtga actttaaaaa gatgagtgct
     1981 gaacttattc gggcaatggc atttgccatt cgtattgtgg ccattgctgt tctggtctgg
     2041 gcaatccgtt ggtggtgata tgaaccgtgt tctgtgtgtg gtgattattg tcatgctggt
     2101 agcctgtggt gtgcttagtc tggggctgaa tcattaccgc gataacgcca tcacctacaa
     2161 agcgcagcgc gataaaaaag tcagtgagct gaaactggcg aatgccacta ttactgacat
     2221 gcagcagcgc cagcgagatg tcgctgcgct tgatgccaga tacacgaagg aattagccga
     2281 tgcgagagct gaaaatgaaa ctctgcgcgc tgatgttgcc gctggtcgta agcgcctgcg
     2341 ggtcaacgcc acctgccccg gtaccgtgcg tgaagccacc ggcacctcca gcgtggataa
     2401 tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg
     2461 gttgatgaca atgcagaagc agctggaagg ggcgcaggaa tatatccgca ctcagtgcac
     2521 taagctggct ttttattatc cggaggatac atgaagaaat tacgggtaac cgtagaacct
     2581 tttcagggaa caattccgtt ccgtattttg cagcgtggtc gtgttcttgt tgaaggttcg
     2641 ttcagtggta aatgtacgca attacactcc cggacctttc aggtgaatgc cacgaatgaa
     2701 gagctaaccg tggagtgtac gatgaatgcc gctaaatgcc gcatggtatt acagccagtg
     2761 tgttgagcga ccttattatc catgcgcggt attgtcgccg tattcctgca ttaacagaga
     2821 ccgcagcccg acagggagac tcctctgcgc gagtgtgcgg ggataatcaa aaacgataca
     2881 caccggggtt taccgcgtta acggagcgcg gcgttgtccc ctcatagtcg cctgtccggt
     2941 gcgatggtgg aagaaaccga acgttcattt ctcgttattt gtcatgctgg ccgggcgcag
     3001 atgcgttgca tctgttgcca gccttctcct gcaggcttca ataacccacg ctgaaaagtt
     3061 accggaccct ttatgctcaa gggcgatgtt gatctgttca attatgtgat tggggaaacg
     3121 gatattgcgg gttgtggttc tgcgggtccg gtttttcgat gacatattta tttcctttac
     3181 tgattgccat atgacgggga ttttacatgg ctgagcttcg tacactccag agcagaatca
     3241 aaacactgaa tacccgacgg gtgaatattc tgaaggggga gcagcgtcgt gtcagtggca
     3301 gtgcacgtgt ttccctcaag cgtcatatct ggctgaggga cgccgggcag tgctgtatct
     3361 gtggtcgtgt ggttgacctc tgtgacagtg aactcgatca ccgaattgca cttcagttcg
     3421 gtggtggtaa tgaggagacg aatctttgga cgctctgtgc cgaatgccat cgacaaaagt
     3481 ctgctcgtga agcggcgagt ggtatgccgg acccgacgct gccggaggtg tccggaggta
     3541 gtggcagaga ggacgacatc atcggactgt aacccaaccc ggggggggta tcatccggcg
     3601 taaaaaacga tcgctttgga caccgcgccc cctctcacgc agagaaaaaa ttcccgtttc
     3661 agggcagtta acatgttaac tggctgcccg ggcatttttg cggtttttat ctttattatt
     3721 cagtttgttg tgcggaaaaa atgttaacag gctttttcag caaatgttaa ccaggcagca
     3781 gttaacattt gcggcatgag acgccgggaa aaatgggctg aaccataccc ggctgagtgc
     3841 gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct
     3901 catgtccggg aaaaacaaaa cagcgtcagc cattgccgcc ggttattcgg cgaagaccgc
     3961 cagggttaaa ggctcgcagc tggcaaaaga tcctgaggtg cttgcgttta tagcccgtaa
     4021 acaatgcgag acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagca
     4081 ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaaacaatct
     4141 ggagatgcca ccgtccgcgg tgatgtctcc tggtattgaa tatatggagg atggtcttcc
     4201 cgatccggtg aaagccatgg ggcggatcct ggtggaaaac ctctgcattg atccgaaact
     4261 ggcactggat gcggcctggc gtctggcgca gttcacgcac cataaaaaag gggataccgg
     4321 gaaaaaatcg gcaaaaggtg atgccgcgaa aaaagcggct aaccgttttg cggtgccacc
     4381 gcccccccga ctggtggtga ataacgataa tgagggcaac ggatgatacc tgtatggagc
     4441 acagcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggatccg
     4501 atttatccgg acgaggccgc acatgccctg gcgattttta aacaactgcg gattgtggat
     4561 gcacctggta gccctacgtt cggggagtcc tgtgcaccgt gggtgtttga cctggtggcg
     4621 gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc atatcaagga agtttttatc
     4681 cttatcccca agaaaaactc gaagtccacg ctggccgcgg ggatcatgat gactgcactg
     4741 ttactgaact ggcggcaggc ggcgggttac acgattctgg ccccgactgt ggaggtggcg
     4801 gccaacgcct tcaaccctgc ccgggatatg gtacgacgtg acgatgatct ggatgacctc
     4861 tgccaggtac agacccatat ccggaccatc acccaccgag tgacggacac caccctgaag
     4921 gtggtggcag ccgatccgaa tacggtgtcc ggtatcaagt ccgtgggtac gctgattgat
     4981 gagttgtggc tgtttggcaa gcagtgcaag gcggaggaca tgttacgtga agccataggc
     5041 ggccttgcct cccgcccgga agggtttgtg gtgtatacga ccacccagtc gaatgaaccg
     5101 cccgccgggg tgttcagaca gaaactgcag tacgcccggg atgtgcgcga cggcaaaatt
     5161 catgatccgc actttctgcc ggtgatattt gaacaccctc ctgaaatggt ggaaagcggg
     5221 actaacctgc tgatggaaaa cctcgccatg gtcaatccga atctcggcta ttcagtggat
     5281 gaggcctttc tgtaccggga gtaccgtaaa gcccgggaag ccggtgaaga gacattccgg
     5341 gggttcatgt caaaacacgc caatgtggaa attggtcttg ccctgcgctc tgaccgctgg
     5401 gcgggggctg atttctggga agagcagggc cgttgtatca gcctggacga tatcctgcgt
     5461 cgtgctgatg tggtgacggt ggggattgac ggcggagggc tggatgatct gctggggatg
     5521 tatgtgattg ggcgtgaccg ggagacccgc gaatggctgg gctggggcca tgcctgggcg
     5581 catgaaaccg cggtggtccg acggaagagc gaggcgtccc ggtttcagga tcttgttgcc
     5641 tgtggagata tgaccattgt ccggcgtgtc ggggatgaca cggcggaagt ggcggaatat
     5701 gtgcgtcgca ttcatgaggc tgagttactg gaccatatcg gtattgaccc gtcaggggtg
     5761 gggcagattc tggattcact ggcggaagcc gggatccccg acggaattgt ggtggggata
     5821 agccagggct ggaaactggg cggggccatt aaaaccaccg agcgcaaact ggctgaaggg
     5881 gtgctggtgc atggtgacca gcccctgatg gcctggtgtg tcggcaatgc ccgggtggag
     5941 cctaaaggta acgccattct tatcaccaaa caggccagtg gacggggaaa aattgacccg
     6001 ctgatggcgc tgttcaatgc ggtctccctg atgtccctta acccggaacc gaaaaagaaa
     6061 gaatatgcgg tttttttcat ataaccctgt tcacactgta accatcacga accgctccgg
     6121 cggttttttt attttcagga ggctgatgtg actcttaaac gggcctgttc cctgctgacg
     6181 gtgaaatcct tcagtgagga tgaacgggtg atcaccggga ttgcgtcaac gccttctccg
     6241 gatcgggatg gtgacatcct ggagccggag ggcgcggagt ttggcagtgc gatcccgttt
     6301 ctctggcagc atgaccattc ccgcccggtg gggcagtgta cggtgcgccg ggtcagcgaa
     6361 gggctggaaa tcacggcaac actggtgaag cccgtaccgg atatgccgtc gcaactggct
     6421 gcccggctgg atgaggtctg ggcggccatt aagaccgggc tggtcagggg gctgtccgtg
     6481 ggcttccgtc cccatgaata cacctttctg gacggaggcg gactgcattt tctgcgctgg
     6541 gaactgatgg aggtgtctgc cgtcaccgtg cccgcgaatg cggaatgcac catccggacc
     6601 attaaatctt acgaccgccc gttttctgcc gcgtccggca accggaaacc ggtggtgaaa
     6661 atcgcatctt ctgccggcgc tgcggcacag tcaacaaccg tttttcataa ggaaaagacc
     6721 ataatgaata ttggcgaaca gattaaaagt tttgaaaaca agcgtgcagc gctggcagcc
     6781 tcccttgagg aggtcatgac caaagccgca gaggaagggc gcacgctgga tgtggaggag
     6841 gaagagcatt acgacaacac cgcagcggaa atccgtcagg tggatgcgca cctgaagcgc
     6901 ctgcgtgaac tggaagccgg taaggccgcc acggcgcagc cggtgaaaca ggccggtaac
     6961 gggaatgtgg ccgcggtggc ttctgcgccg gtgatccgtg tggagcagaa actggataag
     7021 gggattggct tcgcccgctt tgccaaatcg ctggctgcgg ctaaaggcgt ccgatctgaa
     7081 gccctggaag tggcccgtcg tcagtatccg gatgacagtc gtctgcatca tgtcctgaaa
     7141 tcggcagtgg gcgcggggac caccacggat ccgcagtggg caggcagcct gtctgaatat
     7201 caggaatacg cacaggactt tattgattac ctgcgtccgc agaccattat cgggcgattt
     7261 ggtcagggcg ggatccctgc acttcgtcag gtgccattca atatccgtgt gcacgcccag
     7321 gtgtccggcg gtgctgccgg ctgggtgggt gagggtaagg caaaacccct gacgaagttt
     7381 gattttgaat ccatcacctt cagtcatgcg aaggtgtcgg ccattgcggt actgacggaa
     7441 gaattgatcc gtttttccag tccggctgct gatgcactgg tccgtaatgc gctggcggaa
     7501 gcggtggtgg cgcgtctgga tacagacttt gtggacccga aaaaagccgc agtggcagat
     7561 gtctccccgg cgtccatcac ccatgatgtg aagggcacgg catcaaccgg taacccggat
     7621 gcggatgcag aggctgcgtt tggacagttt gtggcagcaa acctgcagcc caccggtgcg
     7681 gtctggctga tgtccagcac caatgccctg gcactgtcca tgcgtaaaaa tgcgctgggt
     7741 cagaaggaat acccggacat gaccctgctg ggtggctcct tccaggggct gccggtgatt
     7801 gtctcccagt acgtgggtga ccagctggtg ctggtgaatg ccccggatat ttatctggcg
     7861 gatgacggcg gcgtggcagt ggatatgtcc cgcgaggcat cactggaaat gcagtctgag
     7921 ccgaccggcg acagtaccac gccgtccccg gtggagctgg tttccatgtt ccagacaggc
     7981 agcgtggcca tccgtgcgga gcgctggatc aactggcgtc gtcgccgtac tgcggcggtg
     8041 gcggtgatca ccggagtgaa ctacggcagt gcgtccggcg gctgagtctg ataaggagga
     8101 cgggaggcgt gcgcctcccg taacaggtta tggcaaagat ccgatatctg cagggcacgc
     8161 atgatgcccg ggccggggat atccgtgatg tggcacagcc gtgtgcggag gtgctggttc
     8221 gcctgggaaa ggcggagtac atcacggtgc gacgtccggc aggtcagaaa aagaaacgtg
     8281 atgcggagca tggcgaatgt ggaacctttt acggcgaacc cgaaaaaacc agaaatcagg
     8341 acgtgacgta agagaggcgg gctggaccag cctgtttcag gcggtggctg agcccttttc
     8401 cggcgcctgg cagcagggcg tgaaagccga tcctgaagcc gtcctctcct ttcatgcggt
     8461 gtttgcatgt atttcgctga tatcccagga tatcgccaaa atgcggctgc gtcttatgca
     8521 gacggatgcg catgggatac gcagggaaac gcgccggggg gatattgccc gcctctgtcg
     8581 tcgtcccaac gcccagcaga accgcatcca gttttttgaa ctgtggctga acgccaaact
     8641 gcgtcatggc aatacggtgg tgctgaaaat ccgtaatgcc cgggggcaga tcaaagaact
     8701 gcgtattctg gactggagcc gggttgaacc tctggtggcg gatgacggcg aggtgttcta
     8761 ccgcatcacg ccggaccgga actgcgggat cacggaggcg gtgacggtgc ctgcccggga
     8821 agtgatccac gaccggttta actgtttttt tcatccgctt ataggattgc cgccggtgta
     8881 tgccgccggg ctggcggcca cgcaggggca tcatattcag gaaaattcga cgtctttttt
     8941 cagaaatggc ggcaggccgt ccggggtgat tgagatcccc ggcagtatta cggaagaaaa
     9001 tgcgaaaaaa ctgaagagca actgggacag cgggtataca ggcgaaaatg cggggaaaac
     9061 ggccattctg agcaacgggg caaaatacaa ccccacgacg ttttcacctg tggatgcgca
     9121 gacggtggaa caactgaaga tgaccgctga aattgtctgt tcggtgttcc gtgtcccggc
     9181 ctacaagatt ggcgtgggac aaccgccttc cagtgacaac gtggaggcgc tggagcagca
     9241 gtattattcc cagtgcctgc agacgctgat tgagtccatt gaactgttac tggatgaggc
     9301 gctggaaacg ggggaaaacg agagtacaga atttgatgtc accacgctgc tgagaatgga
     9361 cagtgagcgg cgcatgaaaa cgctggggga tgcggtgaaa aatacgcttc tcacgcccaa
     9421 tgaggcccgt aaacgggaga acctgccgcc cctggccggc ggtgatgcac tgtatcttca
     9481 gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat gcccgtgagg atccgttcgc
     9541 gtctgccggg aaaacagttt catcacagct gcctgacggc gcatctgacg gtaataaggc
     9601 aatcagtgaa acagagcatg atgcggtgaa agcgatgttc aggggggata ctgagaaaat
     9661 gacggaacgg gaactgtcca ttattcgtgc actgggagaa gaattctcca cagtgctggc
     9721 ggatttacag cgcacatttg aggggaagat ggcctcgcag gcacaagcgt ttgaagagaa
     9781 actgacttcc ctgtcggcgg tattacagaa gcatgtgacg gtggatgagg tgcgtccggt
     9841 tctgcaggcg atggtggatg acgctgtggg ggccattccg gtaccgcgtg atggtcgtga
     9901 ttatgatccg gatgtactgc agcaggcggt gaatgatgcg gtcgcaaata ttccgcagcc
     9961 ggcggacggt aaaagtctca ccccggatga tgtgcgtccg atgcttgaac agatggtgaa
    10021 ggaggctgta agccatatcc ctgttccgcg cgacggtcgt gactatgatc ccgatgttct
    10081 gcagaaggcg gtgaatgatg cggtcgcaaa tattccgcag ccggcagacg gtaaaagtct
    10141 caccccggat gatgtgcgtc cgatgcttga acagatggtg aaggaggcgg taagccatat
    10201 tcatgttccg cgtgatggtc gtgactacga tccggatgtt ctgcagaagg cggttctgga
    10261 tgcggtgagt gccctgccgg ctccgcagga cgggcgtgat gccacggctc tggaaatact
    10321 ccccgccatt gacgatcaaa aatcctttcc ccggggcacg tatgccacac accagggcgg
    10381 actctggcgg gcgtatgaaa aaacgcacgg gatgcgggga tgggaatgcc tggttgacgg
    10441 ggtggcggat attgacgtca gcatgacggg tgagcggttg ttctctgtgg tggtccggca
    10501 gagcagtggc cagcgtacgg aaaaaacatt ttccctgccg gtgatgctct accgcggtgt
    10561 gttcagagcc ggtgaaacct accaccccgg cgatacggtg acgtgggggg gctcgctgtg
    10621 gcactgcaac agtatgaccg aagataaacc cggagaagct cattcatcag cctggaccct
    10681 ggctgcaaaa cgtgggcggg atgcaggagg cggaaaatga cggcattact gacactggaa
    10741 gagatcaagg cacatctgcg tgtcgaccat gacgcggatg atgacatgct gatggacaag
    10801 gttcgtcagg ctaccgccgt gctgctggcc tacattcagg gcagccggga taaagtgatc
    10861 cgtgaggacg gtgaactgat cccgggcgag gcattaaccc ggatgaaggg ggctgccatg
    10921 cgactgaccg ggatgctgta ccggaatccg gatcttgcgg agcgggaaga actgcttcag
    10981 ggggagctgc cgttttctgt ttccgtgctg atttacgatt tgcgttgtcc gacggtgtta
    11041 tgaggagggg gaatggcaat atctgcaggt cgtctgacac agatgataag tgttctgaac
    11101 ccggtgttaa cccgtaacgc tgccggagaa atgacggaag aatgggtgtc atgcgggaaa
    11161 attcatgcgg atatccgagg caggagcagc cgggagcgga tgcagtccgg tgcggaaatg
    11221 gcgcaggcgg aaatccgcat ctgggtgcgc ggtcagtccg gtcgggaaat cacggcagcg
    11281 tcacgacttc atgtgctgag tggtccatgg cgtgaccgga tcctgaacgt tgtcgggctg
    11341 cccgtgccgg atgcgaccgg cgggcgtctg gaaattctct gtcggctggg aggggaaaaa
    11401 tgatcgaaac cctgctggat ttttcggggc tggaggacat cagccgcgat ttgcagcttc
    11461 tgagtggtgc ggaaaataac cgggtgctgc gtgaggcaac ccgtgcgggt gcgaatgtgc
    11521 tgaaagaaga agtggtgtca cgggcaccgg tacgcagggg aaaactgcgc cgcaatgtgg
    11581 tggtcctttc ccggcgctcc cgcgatggcg ggatggaatc cggtgtccat atccgtggtg
    11641 ttaatccgga caccggtaac agcgataaca ccatgaaggc ggataacccg cgcaatgctt
    11701 tctactggcg gtttgtggaa atggggaccg tgaatatgcc accgcacccg tttgtgcgcc
    11761 cggcgtttga tgtgcgcagt gaacaggcag ctcaggtggc gattgcgcgg atgaaccggg
    11821 ccattgatga ggtactgaga cgatgacgga ggcggatttg tatcctcatc tggcgcatct
    11881 tgccggcggg caggtgtacc cgtatgtggt ccccctgctg gatggcaggc cgtcggtggc
    11941 gcttccgtgg gtggttttca gcctgatttc atcggtgtct gcggacgtga tgggcgggca
    12001 ggcggagtcc tcagtgtcgg tgcagataga cgtttatgcc gggactgtga cgcaggcgcg
    12061 tcagatacgt caggacgccc gtgaagccat aatgctgctg gccccgggat ccgtcagtga
    12121 aatgcaggac tatattccgg aaaaccgctg ttaccgtgca accctggagt ttcaggtcac
    12181 ggtgtgactt tttctttttt tctacaaaac ccataccccg ccgcgtgcgg gttttttatt
    12241 atcaggaggc agaatgtctg ctttgtatga acgctcacag ctgacgcagg tgatgatttc
    12301 atctgccccg gcgactgctg aaactatgga taaggcggaa tatctgcgcc tggactgcac
    12361 catcaaggaa gtccagttca ccgctggtca gaaacaggat attgatgtga ccacgctctg
    12421 ctccaccgag caggagaaca tcaacggtct gggggcgtcg tctgagattt ccatgtcggg
    12481 taatttttat ctgaatcagg cccagaacgc cctgcgtgat gcctatgaca atgacgcgtt
    12541 gtatgcgttt aaggtgctgt ttccgtccgg taagggcttt aagttcctgg cggaagtgcg
    12601 tcagcacacc tggtcatccg gtaccaacgg cgtggtggca gcaacgtttt cactgcgtat
    12661 gaaaggcaaa ccggtgtcct ttgtggtacc gctggcgttt gtgaaaaatc tggataaaac
    12721 acttaccgtg aatacaggtg cgctgctgac aatgtcagtc agtgccaacg ggggaacgcc
    12781 gccgtataaa tacgcctgga agaaggatgg tcagccggtt gacgggcaga cgacagacac
    12841 cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa tatacctgcg tggtgaccga
    12901 ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc accgtgacag tgagcgcagc
    12961 cgccggataa ggggatgggt catcatgaaa aaggatctga aaacgctggc gctggccaga
    13021 ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat ggggtaatgt cagcgtggtg
    13081 ctgcgggagc cttcggcaga ggcctggtat ctgtggcagg aagtgctcaa tggtgatgga
    13141 gaggatgacg ataccctgtc ggtggtggcg aaaacccgcc gtaacctaga agcggatgtg
    13201 acgctgttct gcgatgtcct gtgtgatacg gatctgcagc gggtgttcgc tccggacgac
    13261 cgtgagcagg tgctggccgt ctatggtccg gtacatgccc ggttgctgcg tcaggcactg
    13321 gaactgatcg ctgatgcaga gtcggccaga aaaaagtagc ccgcccggaa attcgctttc
    13381 tgatgcgact tgcgctccgt ctggggcgca ccttatccga actgcggcac agcctgagtg
    13441 tgagcgaggc gatgatgtgg atggagttcg acagggtatc cccgctgggt gatgagcgcg
    13501 gggatatccg taatgcacag atcgtgaaag cggtttttgg ggcacagggg atgaatgttg
    13561 cactgaagga cgccatgctc tgctggggcg aggatgagga taagccggag gtggatccgt
    13621 ttgcggcgct ggaagacgcg ctgagcttcg cagcacagtc atgaatgatg agaaccgctg
    13681 aggcggtttt tttacgcccg gagaaaggtg aatggcgacg ttacgtgaac tgattatcaa
    13741 aatttcggca aattcgcagt cattccagtc ggagatccag cgggcttccc gcatgggcag
    13801 tgaatattac cggaccctgc agaatggcgg gcgtcaggct gccgcagtcg cccgggtgca
    13861 gcgacgcgcc ctggctgagc tgaacagcca gttgacggaa atccgcgctt cagctgccgg
    13921 aacggcgggg gcatttgcag gtgcctttgc caccggacac ctgatttctc tggccgatga
    13981 atggagttcc gtgaatgccc gactgaaaca ggcgtcgcag tcatccgatg aattttcgtc
    14041 atcacagaaa gtgctgatgg atatcagcca gcggacgggc acggcatttt cagataatgc
    14101 ggccctgttt gcccgctcgg cagcctcaat gcgtgaatat ggttacagtg ctgatgatgt
    14161 gctgaaggtg acggaggcca tttccacggg gctgaaaatc tccggtgcca gtacggcaga
    14221 ggcgggctcg gtgatcaccc agttcagcca ggcgctggca cagggtgtgt tacgcggtga
    14281 ggaatttaat tcggtcaatg aaagcggaga ccggatcgta cgtgcactgg ctgcgggtat
    14341 gggcgtggcc cgtaaagacc ttaaggcgat ggcggacgac ggcaaactga cggcggataa
    14401 agtcgttcct gcgttaatca gccagctggg ggtattgcgt gatgaatatg ccgccatgcc
    14461 ggaaacggtc tctgacggga tcacaaaggt ggaaaacgcc tttatggcct gggtgggtgg
    14521 cgcgaatgaa gccagcggag cgacgaaaac gctctccggc gtgctgaacg gtgttgccgg
    14581 taatattgat aatgtggcaa cagccgcggg ggcgctggtt gccgtcgggg ttgcccggta
    14641 ctttggcaat atggcctccg gagcggtgtc tgccacggca ggacttgtga cggcagcacg
    14701 taatgaagtg gcactggcgg aagcacagct cagggggacg cagattgcca cggcgcgggc
    14761 aagggcagcc gtgtaccgtg cacagcaggc tgtggcggca gcccgcggga cggagatgca
    14821 gattgctgca gaagcccgtc tggcggccac acaggaacgc ctgaacagaa atattgctgc
    14881 cagaaccgca gcccagaatg agctgaacag tacaacggcg gtgggctcac gtctgatgac
    14941 tggtgcgttg ggactggttg gtggcgtacc cggactggtg atgctggggg cagcagcatg
    15001 gtatacgctg taccagaatc aggagcaggc cagggagtca gcgcgccagt atgcactgac
    15061 gatagatgaa atcgcgcata aaacgccgtc aatgtctttg cctgaagcct cagataatga
    15121 aggacgaaca cgggcggcgc tggcagagca gaaccggctg attgatgaac aggccagccg
    15181 ggtgaaatcc ctgcaggaaa aaatcgctgg atatcagtat gttctggcta accctggctg
    15241 gacaaccggt gacggattca tgataaacca tctgacatcg gtgaagaccg tgacggaagg
    15301 gctttctcag gcaacagagc agcttgccgt tgagcagtcc cgtctggcac agatgcagga
    15361 aaaagcgcag tccattcagg atgtgcttgc cgggctggaa gaccgtcgtg tggcgttaat
    15421 tcgtcagcag gcggcagagc agaataaggt gtaccagtcc atgctggtta tgaacggtca
    15481 gcatacggaa ttcaaccgtc tgctggggct ggggaatgaa ctgcttcagc agcgtcaggg
    15541 actggtgaat gtaccgttac ggctgccgca ggccaccctg gatgataaac agcagagcgc
    15601 cctgacaaaa acagaacgtg agctggccct gtcccgactg aagggggagg aaaaagagcg
    15661 tgcccggctg gggtatgcgg cggatgacct tggttttgtg ggggattcgt atcaggaggc
    15721 gagacagcgt tatatcagta atgctctgga agcctggcgt aataacgagg cgaacaaacc
    15781 taaatcccgg ggtggaaaat cagagacgga aaaagcggaa gacagttttt cccggctact
    15841 gaagcagcag aaagagcaac tggcactggc gggtcagaat acagagctgg cgaagctgaa
    15901 gtaccagact gcgcagggcg aactgaaaac cctgacggag atgcagaagc aggaactgct
    15961 gcgtaacgcg gccctgattg accagcaaaa aatccgggaa cagttgcgat cccgggaaga
    16021 gaccctgaag aatgataatg tggctgcgcg tgcatcaaat gaagccgaac tgctgggata
    16081 cgggcaggga gaacgagcca gggaacgcat gcgggagttg cagcagatcc gtgacagctt
    16141 ccgccagaag gatgcggacc ttcagtctca gtatcagacc ggggatatca gtgaggattt
    16201 ttacagacag gcacgggcac agaacgcgca gtatctgagc gaacgcctta aggaccaggc
    16261 agccttttat gccgaatcgg atgcgcagcg tgcggactgg cagaaaggct tgcaggaggg
    16321 gctcagtaac tgggtggaca atgcatccga ttacgcctca caggcagcac agcttgcgac
    16381 ggagggtatc tcagggatgg tgaataacat cacggagatg ctgaacggaa acaaagtgga
    16441 atggcgcaac tgggcctcat ccgtactgca ggaaatctca aaagttctta tgaatgctgc
    16501 gattgtcaac ggaattaaga cggcggcaaa cggtatgtcc ggtgcgggag gatttattgg
    16561 cagcattggt gactggctgg gcggtgcggt ggccaatgca aaaggcggcg tgtatacctc
    16621 ggcaaacctg agtgcgtaca gcaacagcat tgtggacacg cccacgtact ttgcgtttgc
    16681 aaaaggggcc gggctgatgg gggaagccgg acctgaagcc attatgcccc tgacccgggc
    16741 ggcggatggc tcgctgggcg tacgcgcggt gggcagtatg aacggcagtg ccggtctggt
    16801 gtattccccg gtctaccaca tcgccattca gaatgacggg gctaacggac agataggggc
    16861 ggaggcggca ggcagtcttg tgcagctgat tgaccagcgg gtgcaggcgg tgatgctgtc
    16921 catgcgacgt gacggaggaa tgctgagtgg ctgagataaa aacgctgcat ctggtcccgc
    16981 gtgaagggat gcaggtgagt gagaaaccgt cggtggtgag ggttcggttt ggtgacggtt
    17041 atgaacagcg ccgaccgacg ggacttaatg ccagactgaa gacgtttcag gcggtgttcc
    17101 gggtgacgga tgaaccaacc cggcgctggc tggatgaatt tttatcgtgg catggtggtt
    17161 accgtgcctt tttgtggcga ccgccgaaac ataacctgac ggtgagggtg gtgtgccggg
    17221 agtggagcgt cacagataac gccaggcaca gtgatttcag ttgtacgatt gagcaggtgg
    17281 tgaactgatg caggatattc gcgaagaaag tctgaacgag tcggttaagt cagagcagtc
    17341 accgcgggtg gtactctggg aaatcgacct gacggtacag ggcggtgagc ggtatttttt
    17401 ctgtaatgag ctgaatgaaa aaggggagcc ggtcacctgg caggggcggc aatatcaggc
    17461 atacccgatt gacggcagcg gttttgagat gagcgggaag ggcagcagtg ccagaccgtc
    17521 gctgacggtg tccaatctgt tcggtctggt caccgggatg gcggaagacc tgcagagtct
    17581 ggtgggggcc acggtggtcc gccgccgggt gtatgcccgt tttctggatg cggtgaattt
    17641 cgttgcgggc aatccggagg cggacccgga gcaggagctg agtgaccgct gggtggtgga
    17701 gcagatgtcg cagctgacag ccatgacggc ctcgtttgtg ctggctacac cgaccgagac
    17761 ggatggggcg ctgtttcccg gtcgcatcat gctggcgaac acctgtatgt gggattaccg
    17821 gggagatgaa tgcgggtata acggtcctgc ggtggcggat gagttcgaca accccaccac
    17881 ggatatccgt aaggacagat gcagcaagtg catgcgcggg tgtgaactgc gcaggaatgt
    17941 cggcaatttt ggcggtttcc tttccattaa taaactttcg cagtaaatcc cggtttatga
    18001 cacagactga atcagcgatt ctggcgcatg cccggcggtg tgcgcctgcg gagtcgtgcg
    18061 gcttcgtgat aagcacgtcg gagggggagt ggtatatccc ttgtgtgaat atttctgcag
    18121 agccggaggc gtattttcgt atcgcaccgg aagactggct gcgggcagag atgcaggggg
    18181 agattgtggc actggtccac agtcatcccg gtgggctgcc ctggctgagc gaggctgacc
    18241 ggcggctgca gataaaaagc gcactgccct ggtggctggt ctgccggggt gacattcaca
    18301 aattccgctg tgtgccacat ctgacaggac ggcgctttga gcacggggtg acggactgtt
    18361 acacgctgtt ccgggatgct tatcatctgg cggggactga aatgccggat tttcatcgcg
    18421 aggatgactg gtggcgcaac ggtcagaacc tttacctgga caatatggcg gtcaccggct
    18481 tttaccgggt gcccctgtcc tctgcacagg cgggcgatat tctgctgtgc tgctttggtg
    18541 cttcggtacc gaaccatgcc gccatttact gcggcaacgg tgagctgctt caccatctgc
    18601 ctgaacaact gagtaaacgg gagaggtatt ccgaaaaatg gcaacgacga acgcattctg
    18661 tctggcgtca ccgccactgg cacgcatctg ccttcacggg gatttacaac gatttggccg
    18721 ccgcctcagc ctgtatgtga acacggcagc ggaagccatc cgggcgctgt cgttacaggt
    18781 gccgggcttt cgccgtcaga tgaacgaagg ctggtaccag atacgtattg ccggttatga
    18841 cacggcaccg gaggcggtgt acgcccgtct tcacgaacag ctgggtgagg gaacggtcat
    18901 ccatattgtg ccgcgactgg ccggggccgg aaagggtgga ctgcagattg tgctgggggc
    18961 ggcagccatc gtgggctctt tcttcactgc cggggcatca atggcgttat ggggttcagc
    19021 cctggcagcc ggtggttttt ctgccaccac gatgctgttt tcacttggag ccagcatgat
    19081 tctgggcggt gtggcccaga tgctggcccc gaaggcaaaa acaccggatt accgcgcaac
    19141 ggataacggc agacagaaca cgtacttttc ctcgctggat aacatgattg cccaggggaa
    19201 cccgatgccg gtgccttacg gggaaatgct ggttggctcc cgccgtatat cccaggacat
    19261 cagcacccgt gatgaaggcg ggggcggaac ggtcgtggtt atcgggcgac agggataaaa
    19321 cataaaaaaa tcccgcagtg atcgcggagc tgcggggaca gacaaatgaa gatcaatgtt
    19381 aaggagttgt ttttgttact cgggcaaaaa aacactaacg cagcgaaatt ataagcgcca
    19441 cagtcagtgt gtgaaaatgt gaagatattc agaattttta tgccattacc ggttttaacc
    19501 aacaggatta tcggtgggca tgaaagaaaa ccccggtatc tgctgatacc ggggtttctc
    19561 tttagcatgg cagaaatgtg tttcatgctt ttcgggcgaa ggatatccga cttctgtacg
    19621 gaatggcaag tggcggttaa tttattcagg ggaaggctgt atgggaaaag gtggcggtaa
    19681 ggcacacacg cctcgtgagg cgaaggataa tctcaaatcc acgcagatga tgagcgtgat
    19741 tgatgcgatt ggtgagggac cgatagaagg tccggtgaag ggactgcaga gtattctggt
    19801 gaacaaaacc ccactgacgg acacggacgg caatcccgtg atacacggtg tgacggcggt
    19861 ctggcgcgcc ggggagcagg agcagacacc accggaaggc tttgagtcct ccggagctga
    19921 aaccggactg ggcgtggaag tgacgaaggc aaaaccggtg acgcgcacca ttacgtccgc
    19981 gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca ctggtggaga ccacctcaaa
    20041 gggtgaccgt aacccggcat ccgtccgcct gctgattcag ttacagcgta acggtaactg
    20101 ggtgacagaa aaggacgtca ccattaacgg caagaccacc tcacagttcc tggcctcggt
    20161 gattctggat aatctgcctc cccggccctt taacatccgg atggtcaggg agacggcgga
    20221 cagcaccacg gaccagctgc agaataagac gctgtggtcg tcatacaccg aaatcatcga
    20281 tgtgaaacag tgctacccga acacggccat tgtggggctg caggtggatg cggagcagtt
    20341 cggcggccag cagatgacgg tgaactacca tatccgcggt cgcatcatcc aggtgccgtc
    20401 aaactatgac ccggaaaaac gcacgtatag tggtatctgg gacggcagtc tgaaaccggc
    20461 atacagcaac aacccggcct ggtgtctgtg ggacatgctg actcacccgc gctacggcat
    20521 gggaaaacgt ctgggggcgg cggatgtgga caagtgggcg ctgtatgcca tcgggcagta
    20581 ctgcgaccag acggtcccgg atggtttcgg ggggaccgag ccgcggatga cctttaatgc
    20641 gtacctggca caacagcgta aggcgtggga cgttctcagt gatttctgct ctgcgatgcg
    20701 ctgtatgccg gtatggaacg gtcagacgct gacgttcgtt caggaccgcc cgtcggatgt
    20761 ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg gattccgcta
    20821 cagcttcagt gccctgaagg accggcacac ggcggtggag gtgaattaca ccgacccgca
    20881 gaacggctgg cagacctcca cggaactggt ggaagacccg gaagccatac tgcgctacgg
    20941 acgcaacctg ctgaagatgg acgcgttcgg ctgtaccagc cgcggtcagg cccaccgtgc
    21001 cggactgtgg gtgataaaga ccgaactgct ggaaacgcag acggtggatt tcacgctcgg
    21061 gtctcagggg ctgcggcaca cacccggtga cattattgaa atctgtgata atgactatgc
    21121 cgggaccctg accggcggac gtgtcctgtc cattgatgct gccacccgca ccctgacgct
    21181 ggaccgtgag gttaccctgc cggagacagg tacatcggcg gtgaacctga ttaacggcag
    21241 cggtaagccg gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga tacaggtcag
    21301 taccctgcct gatggtgtgg agacatacgg ggtgtgggga ctctccctgc cgtcactgcg
    21361 ccgtcgcctg ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac
    21421 ggcggtgcag cacgtaccgg aaaaagaagc catcgtggat aacggtgccc gctttgagcc
    21481 gcagtcaggt tccctgaaca gcgtcatccc accggcagtg cagcacctga cggtggaggt
    21541 gagcgcagct gacggccagt atctggcgca ggcgaaatgg gacacgccgc gggtggtgaa
    21601 gggtgtgcgc ttcagtctgc gcctgaccag tggtaaggga acggatgcca gactggtgac
    21661 caccgccatc accgcagaca cggagcaccg tttcagcggc ctgccgctcg gggaatacac
    21721 cctgacggtg cgggcgataa acagctatgg ccagcagggt gaacctgcca ccaccacctt
    21781 ccggattgcc gcaccggcag caccgtcgcg gattgagctg acgccgggct attttcagat
    21841 aaccgcaacg ccacatcttg ccgtttatga cccgacggta cagtttgagt tctggttctc
    21901 ggaaaagcgg attgcggata tcaggcaggt tgaaaccgca gcccgctatc ttggctcggc
    21961 gctgtactgg atagctgcca gtatcaatat caaaccgggc catgattatt atttttatat
    22021 ccgcagtgtg aatactgttg gcaaatcggc attcgtggag gctgtcggtc gggcgagcga
    22081 tgatgcggaa ggttacctgg attttttcaa aggagaaatc gggaaaacac atctggccca
    22141 ggagctgtgg acgcagattg ataacggtca gcttgcgccg gacctggctg aaatcaggac
    22201 gtccattacg aatgtcagca atgaaatcac gcagaccgtc aataaaaaac tggaaaatca
    22261 gagtgcggca atccagcaga tacagaaagt tcaggttgat acaaataata acctgaacag
    22321 catgtgggcc gtgaaactgc agcagatgca ggacggacgc ctttatattg cgggtatcgg
    22381 tgccggtatt gagaatacgc cagcaggaat gcagagtcag gtgctgctgg cggcagacag
    22441 gattgcgatg attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga
    22501 tcagatattt atgaatgaag tgttcctgaa atatctgacg gctcccacca ttaccagcgg
    22561 cggtaatcct ccggcatttt ccctgacacc ggacgggcgg ctgacggcga aaaatgccga
    22621 tatcagcggt aacgtgaatg cgaactccgg gacgctcaac aacgtcacga ttaacgagaa
    22681 ctgtcgggtt ctgggaaaat tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt
    22741 gggcaaagct ttcccccggg actcccgtgc accggagcgg tggccatcag gaaccattac
    22801 cgtcagggtt tatgacgatc agccgtttga ccggcagatt gttattccgg cggtggcatt
    22861 cagcggcgct aaacatgaga aagagcatac tgatatttac tcctcatgcc gtctgatagt
    22921 gcggaaaaac ggtgctgaaa tttataaccg taccgcgctg gataatacgc tgatttacag
    22981 tggtgttatt gatatgcctg ccggtcacgg tcacatgaca ctggagtttt cggtgtcagc
    23041 atggctggta aataactggt atcccacagc aagtatcagc gatttgctgg ttgtggtgat
    23101 gaagaaagcc actgcaggca tcacgattag ctgaatttta taacccagat acgggcgcca
    23161 gaaatggcgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa ttatgtgctg
    23221 ttattttgtc cgcagtagtc tggcaggtcg ccgctgctac gccagcgagt gcagcagaac
    23281 atcagtccac gctgagcgcg gggtatctcc atgcctcgac gaacgttccc ggtagtgatg
    23341 atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacgcgctg gggctgatta
    23401 cgtccttcag ttatgccaat gctgaggatg agcaaaaaac gcgctacagc gatacccgct
    23461 ggcatgaaga ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtacgcg
    23521 tgaatgaatg gttcagcgcg tatgcgatgg cgggtgtggc ttacagccgt gtgtcgactt
//
LOCUS       DAPQZD010000067        23387 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPQZD010000067 DAPQZD010000000
VERSION     DAPQZD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.067"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            42..788
                     /locus_tag="RO454_002558"
     CDS             42..788
                     /locus_tag="RO454_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDY0934376.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QHGQTIMSELVQGRNITITAPLAESVLKNMVDLMPGSTLNEDDNAVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGRMS
                     GPKPVKTVSIKPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINPAD"
     gene            844..1161
                     /locus_tag="RO454_002559"
     CDS             844..1161
                     /locus_tag="RO454_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934377.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            1287..1511
                     /locus_tag="RO454_002560"
     CDS             1287..1511
                     /locus_tag="RO454_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934378.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            1519..6069
                     /locus_tag="RO454_002561"
     CDS             1519..6069
                     /locus_tag="RO454_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDY0934379.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     DSGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRVM
                     LRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKAL
                     VDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGITM
                     GELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADRV
                     GQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELVI
                     NLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRAA
                     RMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTML
                     RSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNSAG
                     VITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQI
                     FAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLSS
                     RKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERDN
                     AAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEGK
                     PIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSAN
                     ASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTDL
                     ANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPEADSIISAG
                     YTPSREDKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKET
                     GTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITNM
                     VVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEAT
                     KETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQN
                     LSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSVTSSSAKTAT
                     SSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAYVP
                     LPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMKAI
                     ALETIAQEKRPGGSLNPNTQRN"
     gene            6112..6447
                     /locus_tag="RO454_002562"
     CDS             6112..6447
                     /locus_tag="RO454_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0934380.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            6530..7228
                     /locus_tag="RO454_002563"
     CDS             6530..7228
                     /locus_tag="RO454_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDY0934381.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            7221..8018
                     /locus_tag="RO454_002564"
     CDS             7221..8018
                     /locus_tag="RO454_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDY0934382.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASYADRAGCETTEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            8006..8596
                     /locus_tag="RO454_002565"
     CDS             8006..8596
                     /locus_tag="RO454_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDY0934383.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAVLMVASIWVPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFDG
                     PQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            8614..13338
                     /locus_tag="RO454_002566"
     CDS             8614..13338
                     /locus_tag="RO454_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDY0934384.1"
                     /translation="MEQFKKKKLPLLIAGAGGKKSKSSSRTPVEADDTVNSRAMAAIL
                     DLLGEGVIGGLVNGAKSIFIDDLPILNEDGSSNFSGITWDFRDGSQDQTPMAGFDFVE
                     TPKSVNIQLKKTHDVTVSIDNDEADRVRVIMKFPSLRSVDKSSGDTNGTTVKYKFQIA
                     NGDATFSDVVAEGEKSVDITLTAKKTGVYYRSYELKLPKPGRAYKVRVIRLTDDNSSQ
                     YLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGSTMPSRSYLVRGIKIRVPSNY
                     NEYTNTYVGVWDGTFKLLSSSNPAWILYDLLTNTRYGLGQFVSESMIDLGQLYQIGRY
                     CDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSPS
                     DPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRYG
                     ERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNKY
                     RAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKVT
                     FSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEAI
                     DNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISWR
                     RSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGTM
                     TPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTFT
                     HAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLAGKITSSELGQALLE
                     DINSKASQEAVDELNEHINQSVESLEGSVNDVKEDIAELDKQFSDNLAGFEIKFNERS
                     DALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIKA
                     ARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAEL
                     TTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEIG
                     EDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTTQI
                     TQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARASG
                     DSALAKQINQLQSKVDGDISAAITQEQEARASADQALSREINSLRAQTGTDIAAAVAV
                     ETKARTDADSALSTQISSLTAKANDLEASLARETTARADGDTALTKEVSSLKAQTAKD
                     ISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQITN
                     LESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTATIQQQATAIADTNKK
                     VSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFVI
                     ENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRLV
                     INGQRIDVYDDNNVLRVRIGLL"
     gene            14793..14975
                     /locus_tag="RO454_002567"
                     /pseudo
     CDS             14793..14975
                     /locus_tag="RO454_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            15501..17588
                     /locus_tag="RO454_002568"
     CDS             15501..17588
                     /locus_tag="RO454_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDY0934385.1"
                     /translation="MTGTLNGNASTATKLQTARSIGGVGFDGSANINLPGVNTTGNQN
                     TTGNAATATKLQTARTIGGVSFDGTANINLPGVNTTGNQNTTGNAATATKLQTARTIN
                     GVSFDGSANISLSPANIGCPASPTGWLTTGSNGGAITTAQLVTLLQNNGAFNTKSWIA
                     RCAWAYANSATIPNSETGCGVIPLAGAVIEVFNNGSSSNNYTIRITTATTTSVSGALT
                     NAEFIYVFNGTDYSPGWRRVYNTKNKPTASDVGALPLTGGTLSGGLTSSGEIISKYAN
                     GFRIAYGSFGFFIRNDGSNTYFMLTASGDTLGSWNGLRPITINNTSGAVSIGNGLNVT
                     GGVNGSLNGNASTATKLQTARNINGVKFDGSGDININTLVSRGRVTALSGSTQGTAGI
                     QMYEAYNNSYPTMYGNVLHMKGASAAGEGELLIGWSGTSGAHAPVFIRSRRDNTGAAW
                     SAWAQVYTAKDSIPGVNTTGNQNTTGNAATATKLQTARKIAGVAFDGSADITLTAANL
                     NAYTKTEVTNLLSSYASRSSLTGYSGNLDIIAETLVVKSGGSGGFAIWDIGTTTSGAN
                     MYIDPNPGINTVWRSTSSRRYKKDIETLQDRYADELLSLRPVWYRSICRGDRKDWGYY
                     GLIAEEVGEIAPQYVHWREPTNNDSPEDISSNGMVAEGVMYERLVVPLIHHIQQLTKR
                     VEELETKLNSPKE"
     gene            17649..17903
                     /locus_tag="RO454_002569"
     CDS             17649..17903
                     /locus_tag="RO454_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934386.1"
                     /translation="MSYGAQVWSPSRQEMVDALAPVYYLDYFTPSGSGSRTYEVEAGL
                     GIDYYIMDVTNGKYTSLTVSGNTISWSGASGNLNILVFQK"
     gene            17903..18511
                     /locus_tag="RO454_002570"
     CDS             17903..18511
                     /locus_tag="RO454_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934387.1"
                     /translation="MYGSKIYRSDGKVWMSPSLSPIVFQRKQVVSLSGGTEFNTQISP
                     DRSPMIFVAYSKAVSLIANRIVRNNQVIYSFGGQGSDSSATIYVFSKGIAKKETWGMS
                     FFNAQGEEIYNTANIPLSFTFLNNTEWNSSGGHVFDYPPAIIPTYANVFAVPVPGGAM
                     TMVYGYAAYGNTVSSIFVNQLNGGHSFSVNGRVPVINRNLYN"
     gene            18834..19157
                     /locus_tag="RO454_002571"
     CDS             18834..19157
                     /locus_tag="RO454_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934388.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KNGLQ"
     gene            19171..19863
                     /locus_tag="RO454_002572"
     CDS             19171..19863
                     /locus_tag="RO454_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934389.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRRHQKGMV"
     gene            19865..20116
                     /locus_tag="RO454_002573"
     CDS             19865..20116
                     /locus_tag="RO454_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934390.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETVNEVRQKDASGVHRELFDKWVRD"
     gene            20489..20908
                     /locus_tag="RO454_002574"
     CDS             20489..20908
                     /locus_tag="RO454_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934391.1"
                     /translation="MLNYHDNTRSMQTIRTNTAVIDSFPMRIHNSEDAVEVRRMLCRE
                     SANRQHFIVTFKSDVARAEKISNSTSLVKPLAEVVANGKRTSFVLDVAEQYPDLKCLD
                     RDISSRIKQVVESFMKKFCKIDDMEPSTAQNTSWHHL"
     gene            20893..21666
                     /locus_tag="RO454_002575"
     CDS             20893..21666
                     /locus_tag="RO454_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934392.1"
                     /translation="MAPSLIGQANRLIPEIEDYSKVLSEMPRATRIIALGEFYFISRG
                     FDLIRFTEYELVDSAYERCIVGECLESCDPDYQEQEVKRLKRAGYTPLEIGAEIESYK
                     LHVGQPVQTVFRVAYCEFPIKDKSGVKVSAKQIRGVFTHADYMGLGIASFAYLYLVDK
                     YQYLVCDTLQTVKGATLWAVSMRKHGLVEIYDTKNENFIEELGEDACGVSGFIPWDIG
                     KSDGGQMRRREEWGTNKINLTPGACTHIVNILTKRSMNQ"
     gene            21817..22485
                     /locus_tag="RO454_002576"
     CDS             21817..22485
                     /locus_tag="RO454_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0934393.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            22491..22844
                     /locus_tag="RO454_002577"
     CDS             22491..22844
                     /locus_tag="RO454_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934394.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKTPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            22889..>23387
                     /locus_tag="RO454_002578"
     CDS             22889..>23387
                     /locus_tag="RO454_002578"
                     /inference="COORDINATES: protein motif:HMM:NF022633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HDY0934395.1"
                     /translation="MLYKYIGSEDSQKVLRNLKRFVEDGTISASAPSSFNDPSEFKVS
                     ITMGGTEVERRKHFEDNGCKDFSFEDWDAVAPEAAKSLAVDLRKESLGAFGVVCLSPN
                     EDNILMWSHYSVSHKGFCIGFDDDFVTSIEGYHSFDNVKYIPSVPVFDEVTDEFKVLF
                     DKLFFY"
BASE COUNT         6595 a         5109 c         6024 g         5659 t
ORIGIN      
        1 aatgaatgcg tagaattaac ggaaaaagga gttttccatc aatgtctaat acccatgtaa
       61 aaaacatcaa acttggtgcc tgtaaggtgt cgtttggtgg tgtggatttg ggttacacca
      121 aaggtggtgt tcaggttgaa gtagccactg aaacgctgaa agtcactgtc gaccagcacg
      181 gccagacaat tatgtcagag ctggtgcagg gtcgtaacat taccattact gcgccgctgg
      241 ccgagtctgt gttgaaaaat atggtcgatc tgatgccggg gtctactctg aacgaagacg
      301 acaacgctgt gaccatcact tccgcacagg gtgtcaacct gatcgacgta gccaaagagc
      361 tggttctgac tccgcaggac actaccgact acgttctgac catcccgaaa gctgcgaccg
      421 caggtaactt caccatgact taccagtctg atgatgttcg tgtgttctct gttcagttca
      481 gcgcataccc tgatgacgaa ggcgttctgg gaagaatgag cggccctaaa ccggtaaaaa
      541 ccgtgtcgat caaaccggaa tctccaactg taaaagcagg ggagactgtg caactgacag
      601 ccgaaatcac tccagctgat gccgcagaca aaaccggtgt ttgggaatcc gaagaccagg
      661 aaaaggccac ggtagatcaa acgggtctgg tacgtggtgt ggcgcagggt tctacaaata
      721 tctccttcac cagcaatagc ggtggtaaga aggcaactaa gtcagtaacg attaaccccg
      781 cagattaatc agtaattaag cagaggctca ggatgggcct ctcttttaaa aggattttaa
      841 ccaatgacca aattactcga tctcgattcc attctgcctc cgaagaaaag tatcaaattt
      901 ggcggtcagg aatatcccat cgttgaaatg acggtaggtc tgttcgtttc catcaagcag
      961 atggaaggca aagatctcat gaacatgtct cctgttgagc aagtgacagc ttatgcagat
     1021 ctggttcgta aggttatccc ttcagtacct gacgaagttc ttgaaaaact gactgttccg
     1081 caactccagc agatcttcac cttcgctatg gaagtgattg atgaagaaaa cgaaaaagcg
     1141 gctggcgaag gggcaaagta atttcccgcg atgaatccgg gacaaggacc gtttcaatag
     1201 atctcggatt ctatttcagt cgtgtagttg ctcactacgc cgtgtcgcca ttagagctat
     1261 tgaacatccc tctcacgatg ttctggatgc tcagtcgcaa tatagaccgt ctgcgtgcgg
     1321 aagaggatgt ccgcaactta caagtcgctc gtgcgtccca ggcggatggc gaggctgtga
     1381 aggcgttcat ggagggtttg caactcagga ttggaagacc agtcgtaaca gataaagtct
     1441 acgatccaag ccaggataag gcagaccctg acgccaaaga gcaactgatg caaatatttg
     1501 gcagaggatg acaagggaat gtcacaaaac gtagagttta tcctgtcgct ggaagacaaa
     1561 cagtttacag cgtcaatcga tcgtgcgggg aaactgctta ctcgatttgg tgagcaggta
     1621 acaaagcctg ctcaaaaaat ccaaaccttt gaacgctctt tgggttcggt cgcccgtatc
     1681 attggcgttc tggaaagcaa gctcgattct acggcagata aactacagga tgtagctgcc
     1741 ggttttgagc ttgcttcaga tgctacgcgt aaaatgcgtg gcaacatcac cagcctcaat
     1801 tctggtctta aagccctgat tgagcgcgtc gatacgacaa cttcttcagt agataaactc
     1861 accgcgtcat tacgtaaagt gcaatcagag ctaaatgatt tctctgattg ggcgacctat
     1921 gcaagcaaaa gcgcaagccg ctttggtacg gaggtcaaag aagcctctgc gtccgtgagt
     1981 ggcatgaata cgcgccttaa taccacgacg aagcgactca gtaattgggg tgtcacaacg
     2041 agtcaggctg ccgagggact gaaaaaggtt cgtgagcaga tggacgaagt tatcggacgt
     2101 cagcaactga ttagcaagcc ggtacgcgtt cgtacatctg gaagtggcga tggtggcagc
     2161 gggcggcgta gcgactctgg ccatagcggt aaaagtaacg aaggaggtat gttctctggc
     2221 cttcgtggca acattttcct gcttggcgag atcggagatg cagccagaac ggttactgac
     2281 atcatgtttg gatggcagaa gcctattgtt gaagctgcgg ccgaaatgga acgtatgcgg
     2341 gtgatgcttc gtgggttgaa taaggagaag tctaaccctg gccaggctgc cgccgatgat
     2401 atgaagtaca tcgtagacat ggctcaaaat gcgccgttcg cgatgcaggc gttaacagat
     2461 tccttcgtga agtttcgttc tgctggctta gatccaaccg atggctctct gaaggcgctg
     2521 gtggactccg ttgctcgttt tggtggtgat agtgagctgt tgaaacgtgc ggctgtggcc
     2581 gttcagcaga tgtccggtaa gggcgtcgtg tcaatggaag agctacgtca gcaattaggt
     2641 gaagccgttc ctaacgcgat gcaggcaatg gcagacgccg caggcatcac tatgggggaa
     2701 ctgactaaag ccgttgccag cggtacggtt gaggcaaagc aggcgttgtc tctgatgttt
     2761 gttggtctgc gtgcggagaa cgagaacgca gcgaaagaca tgatgcaaac ctacacaggt
     2821 gcgctggcac aacttcagac ctcatttacg ctatttgcag atcgtgttgg tcaggccgga
     2881 tatctggatt ctctatcaaa ggggatgaaa gaactggctt caatcatgaa tagcgcggaa
     2941 gggatttcgt ttgccaattc tttaggttcg gggttaacga cggcaatcga tgggttgcgt
     3001 cagcttgctc aatggttagc aaagaaccaa gagttggtaa ttaatctcgg taaggttgtg
     3061 gccgcgatgg ttgcgttcaa actgatgcga gcagggatca tgggggtagt tggcgctggc
     3121 agccagatgg ttagcacctt tgccacgatg gcgaccgcta tacagactcc atttaacctc
     3181 ggcgctacag cagtaactcg attcaatcgt gcggcacgta tggggctggc tccgatcccc
     3241 tctcttattt tcgccatccg tggggcgatt acggggctta aaggcgcttt tgcaggatta
     3301 acggcgttca ttgcggcaaa ccctataggg gcagcattta cagtcgccac tgtagctgtt
     3361 gctggcctaa tcacgtacat gaccatgctc cgcagcgaaa cttcaaaggt cgttgacgag
     3421 attaggaaaa taccagaggc gatgacggcg gccaaacgtg cacaaatggc cgaccgcgca
     3481 gaaaagctcg aaaagcagat tcttgctgat caacgagcat taaaaactgg tgagggtgtt
     3541 aattacgtat ctaactctgc cggtgttatt actcacaaag agtcgaaggc tgacatcgaa
     3601 gcacgcctga aaaaaaatca ggaggagtat caaaagataa ccggcacgat agctcttggt
     3661 gacggcgctg tagcaaagcg tttggctaaa gaggctgccg aatctcagat tgagaaaatt
     3721 cgagcggaaa accagatttt cgcggcaaca ttcgtgaaag cccggcaaga ggctctggat
     3781 aagatccaga aaatcaatga tgacggttca ctttcagatg acgaaaagaa caagctattg
     3841 gcaccgttac gtgaaacggt aaacaaaagc tatctggagc ctgcgcaaaa actggttgat
     3901 tcactttctt ctcgtaagaa tgcgaccgag aagcaaattg cgactcttaa tgatcagctt
     3961 gaaaaagcca aaaaggatgg aaataccgag cagatccaga aactgcaagg cagtattcgt
     4021 ggttatcagg agcatttgga agccgttgct caggagctga ctcaggcaga gttcgagaga
     4081 gataacgcgg ccaaaactgg taagggcgta atgtcaaacc aggggactgt tcttgggtta
     4141 ggtacaactg ataaagctgc tcagaaggcg ctggcgcaat atatgcgaaa ccagatggat
     4201 tctgcgactt atcaacgtac tttgcctgac ggcactccga tgatggactt cgaaggtaag
     4261 ccgattattg ggcctaaaca actcaagacg cagcttaatt tgcagaaagc atccagcgcc
     4321 agctctctgg agaaaatgag cgatgaagag cgtgccgcag ccattgctgc actgactaaa
     4381 gctcgtgaac aggatgcagc agctgccgag aaagcagccc aacgatcagc taacgcctcg
     4441 cagcgtgcgg ccaagaagga acaggcagcg caacagaaac tggcagccgg ataccagaag
     4501 gctctggaca aagccgatca gcttatgggg caaatgggtg aaagctctaa ggctacggta
     4561 tcgtttgatc agtctcttcg cgatacaacg aaatcgctga cagatttggc taatgcggtt
     4621 cctaacgagt tcatcactca agagatgatc gacaaggcga aaaaacgtct tgaagacctc
     4681 aaaaatgcga cacctgaata tcgcgagatg tttaatcgcc gcaatgttga gcagatgatc
     4741 tccacttggg caccggaggc ggattccatt attagtgctg gctatacgcc gtctcgcgaa
     4801 gataaagttg ctgatttcga agacacctac aaccgcaatc tcaaagcgtt gatagaactt
     4861 cgtgataagg cgtctgatcc taaagttgtg gcgctttata caaagaaaat caatcaactg
     4921 attgctgctg gcaataccgc gcttattaaa gagacgggga ctgcgacgca gaagttggca
     4981 ctggaatacg aaaacctggc agagcagatc gaaagcacct ggactgattt gtttagtggc
     5041 ttaactgatg tcctgactga tttcgttatt aacgggaaga tgagcttctc cagcctatct
     5101 cagtccattt tgaaagatat caccaatatg gtcgtgaagt cgcagatcac gctgcctcta
     5161 atgaacatgt tggggatggg aaccaccgca gctggtagtt cacagagtgg taatttgctg
     5221 accggggttg cttccgccgt tgccaatcaa ggggtacgaa tgggcaacac tgttaacggc
     5281 gacaagtcgg taggagaagc cacgaaggag acgtccagtt cggtaactgg attggggcaa
     5341 acaacacagc agaccaccag cgcaattggc actgcaacaa atgcgattgg tagctgggta
     5401 tcagggctat ttgatagcac tgaagccaaa gatgccgaga caaaagccgt gaaggactcc
     5461 atcttctcga tgcagaacct cagctctgtt accggcgcgc tgtctgccgc gtttgcaatg
     5521 cttggagcta atgcttccgg ctctggtaat aagtggttga gttttggagc aacggttgca
     5581 tctggtcttg tttcagcttg ggctggcggt ggtttcgaca gcgtgacatc aagctctgct
     5641 aaaaccgcaa ccagcagtgt ggctgacgga actaaaggca ttcctgcaat cccgaagttt
     5701 gcaaatggag gaatattcgg aaaagacggc gtgatcccgc tccgggcata ccagaaaggc
     5761 ggtattgcta actcgcctca attagcgttg tttggggaag gttctatgaa tgaggcgtat
     5821 gttccattgc ctgatggccg aacaatccct gtaacgctca gtaccgatgg tatgagtgga
     5881 ggcggaaatg ttctttctcc tgtatcaatt gagatcaacg tccatagtga cggtagcacg
     5941 acagaatccg gcgatacaga aagcatatgg aacaatgccg ctcaacggat gaaagcaatc
     6001 gcgcttgaga ctatcgctca agagaaacgc cctggcggat cactcaaccc aaacactcaa
     6061 cgtaactaac tatcgactgc cccgaccggg gcagtctcac aaggatgtga gatggaaaga
     6121 caaacgttta attggtatcc agattacgaa tctgaaaaga gcgtaaaacc gaatgtaacg
     6181 gtacttaatt ttggtgatga ctacgagcag cgacaggctc aaggtcttaa tcgtattaaa
     6241 gaagaatggt cgttaacctt taccagatca tacaacgaaa ttaatgcaat cgatgacttc
     6301 ctgactgagc gatcaggtgt tgaatcgttc tattgggtta atccaagagg caagcagatt
     6361 gtagttgtat gtgacagtca tacggtcaag cgatatcagg ggtactgtgt cttaactgct
     6421 acattcagac aagtatttga ggcttaagca tctggataag taagtactaa tttactatca
     6481 ttgtggcgct gacaggatgt tagcgcctac ttatttcaag gatgaaacaa tgggaattaa
     6541 agctgatatt cagagcttat ctccctctgc actcattgag ttgttcgtat tggatatgtc
     6601 gaacacaact tcagggggga agctattctt ccacgccgga acaaacgaac tgatgcaacc
     6661 ggtcgtctgg caaggagtga catacgagcc gtggccaatc aaagcatcag gctttgacaa
     6721 aactggccag ggaacgttgc cacgtccaaa aattcaggta tcgaactttg ccggaaccgt
     6781 ctctgcggaa gttcaggcga acgacgatct tgttggctgc cgcattattc gcaagatgac
     6841 gctggctcgc ttcctcgatg ccgttaattt taaagacggc aacccaacag cagatccaaa
     6901 ccaacatttc ccggatgaaa tgtggtttat cgaacagaaa actctcgaaa ctcatcaggt
     6961 tgtcgagttt gaattgtcca gtgtgttcga tttgatgggg gtgcaactgc cgtatcgtca
     7021 gatcattaaa aacacctgcc cgtggaaata ccgagggcca gaatgcggct ataccggtcc
     7081 atatttcgac aaaaataacc agcagacgtc tatgtctggt gcggattact gcacaaaacg
     7141 ttatgacgcc tgtaatgcgc gtcggaatta ttttgccgac ggtgtgatcc attttggcgg
     7201 atttattgga gctacgcggt atgggtaata aagcaatccc tgagcttggc tctgacgtta
     7261 tgcagcaaat ctatctctgc gccataaatc gctaccctaa tgaagcgtgt ggctttctgg
     7321 ttagaactaa tggcgacaaa tatcgcttta tggaagcgcg gaatgtttcg gagaacccgc
     7381 agaacacttt tgtaatgcac gttgacgaca ttatggcggc agaggatgcg ggtgatgtta
     7441 tcgcaatctg gcattcacat actgatgaat cagcagaagc atcttatgcc gatcgtgcag
     7501 gctgcgaaac gacggaagtt ccgtggatga ttctggctat tcgcaagaat gttgagggag
     7561 atgccccttt ccattttagc gagatgaatg tgatcacacc tgatggtttc gaaatgccat
     7621 acctgggcag accgtatgta tttggcgtat ttgattgctg gatgttgtgt cgggactatt
     7681 tgaagcgtga gtttaacgtc gaactaaacc cgaacgcaca cctgcatatt ccatcgtggt
     7741 acaccggcga taacgacatt ctcgatcaga actaccgaaa cgaaggattg gtacggcttg
     7801 cgcccggaac agaacctcaa cgtggggacg tcttctttat ccaatacgga aaaatgcctg
     7861 accactgtgc ggtttatatc ggcgatggaa tgattcttca tcaccagatc gaccgcctta
     7921 gttgtcgtgc ttattacggc gggatgtatc aaaaacatac gacgcatcac ttgcgtcaca
     7981 gagacttgct caagggagat gagacgtgtc tgagttagtt catgtgcagc ttggcggccc
     8041 tatggccaaa cattttggcc gccactggca tctaaaggtg cgtaatacaa aacaggctct
     8101 ggatttaatt gaggccaaca agcctgggtt taaagcatgg atgaagcgca atatcaaaac
     8161 ctatgacaga taccacatcc agatcaccaa taaacagggc cacaagtggt ctgtggacga
     8221 gagtgaatat cagatgatgg ggcagtctga caatattgca aaaatccgca ttacccctgt
     8281 tccgcgagga agtggtggat ctgcttttgg atggtttcag acggttgttg gagcagtatt
     8341 aatggttgcc tcgatatggg tgcccgctct tgcgcctctc ggtttgtcac tgatgatggg
     8401 cggtatagcg caaatcatat ctccgcaagc cactaacgaa agtgtgaggc aggcggataa
     8461 ctcgaactct tattactttg atggacctca aaacacaaca aaccagggga accctgtaca
     8521 gcttatctat ggcgaggaaa ttttagttgg ctcacaggtc gtgagttctt ctatcaccat
     8581 cgaccagctt atgtaatcaa ggattttttg gacatggaac agttcaagaa gaaaaaatta
     8641 ccgcttctta ttgccggtgc tggtggcaag aaaagtaaaa gctccagccg taccccggtt
     8701 gaagctgatg atactgtcaa ttctcgtgct atggcagcca tcctcgacct tcttggcgag
     8761 ggggttatcg gtggcctggt aaatggcgca aagtcgatct tcatcgacga tttaccgatc
     8821 ttgaatgaag atggctcttc caacttcagc ggtattacat gggattttcg tgacggctca
     8881 caagaccaga cgccaatggc tggttttgat tttgttgaaa cgcctaagtc cgtaaatatt
     8941 cagcttaaaa aaacacacga cgttacggtt tcaatcgata acgatgaagc tgatcgcgtt
     9001 cgtgtcatca tgaaattccc ctctttacgt agcgttgata aaagctctgg cgatacaaat
     9061 ggcactacgg tgaagtacaa attccagatc gccaacggag acgcaacatt ttctgatgta
     9121 gtggcagaag gtgagaaaag cgttgatatc acgctaacgg caaagaaaac aggcgtgtat
     9181 taccgtagct atgagctgaa actacctaag cctggtcgtg catacaaggt tagggtgatt
     9241 cgtcttacag atgataacag tagccagtac ctctacaacg acacatgggt tgattccata
     9301 ggtgagattg tcgacacgcc aatgaactac cctaactctg ctcttgttgg cttgaaggtc
     9361 aattcagagc agtttggtag cacaatgcct tcccgttcgt atttggtgcg cggtatcaaa
     9421 atccgagtac cgtcaaacta caacgaatac accaatactt atgtcggcgt atgggacggt
     9481 acttttaagc tgttgtcttc ttcaaaccct gcgtggattc tctacgattt gcttaccaat
     9541 actcggtatg gtcttgggca atttgtgtcg gaatccatga ttgacctcgg ccagctctac
     9601 cagattggtc gatattgtga cgaagaggtc gatgatggct ttgggggtaa agagaaacgc
     9661 tttgcaatca atacgcagat caccagtcga caagacgcat accgattaat tcaggatatt
     9721 gccggtgcat tccgcggcat ggtgttttgg gctggtggca tggttaacat catgcaggat
     9781 agcccatcag atccggtaat gatgtttacc aactccaacg tcaaagatgg actgtttacc
     9841 tataaaggtt ctgcgcgtaa agatcgccca tccgttgcgc tcgtaaccta caataacaag
     9901 gaggacggtt ataagcaaaa catcgaatac gttgaagatc aggacgcaat gcgccgttat
     9961 ggtgagcgta aaacagaagt cgtagcattt ggatgtacaa gccgaggcca ggctcaccga
    10021 gttggtttgt ggcttttgta taccgccaga atggagtcgg atgtaattac atttactgcc
    10081 ggcttagacg cctcatttct gatgcctggt gaaaccgttc tgattcagaa caaatatcgt
    10141 gcaggtaaac gtaactccgg tcgaattgtg gagtttacca aaaacagcat cactctcgat
    10201 gcgcctgtgt cgttagctaa aagcggcagc tttattcgga tattgaatca ggaaggcaaa
    10261 atcgttgaac gcgatgttct tgaaactggc gaaaacataa caaaggttac gttttcaaaa
    10321 gccctgtcgt cagcggaaac gcctgttttg aatggtgtct ggacaattac agaaccagat
    10381 ctcgaaccta tgcgcgttcg catcgttaac atcgcgcagg gggaaacgcc gggtagcttt
    10441 gacatcaccg ctgttgagaa caatccgtct aaatatgagg caatcgacaa tggcgcaacg
    10501 ctgatcccgc agaatacgac ggtattggac ccaacttact ccaagccatc taatctgcaa
    10561 atcactgaag ggacttatct ctcaagcccg ggcaacctgt cagtaaaact gactgcaaca
    10621 tgggaaggaa aatctccaga gtattggatc agttggcgac gttctgatga aaacaatgta
    10681 tcgaactggc aatcggcgcg tgtaaccgaa gagcaatacg aaatcgttaa tgtcgcggag
    10741 aatggacgct acgacttcca actgtatgcg gtttcattca acggtaaaaa aacagagatt
    10801 atcagtaccg tttatcaggt gttgggcaca atgacaccgc cggatgcccc aacgtcatta
    10861 acggccgttg gagactatcg taatgtgata ctgaattggg ttaatccaga ttcggtagac
    10921 cttgatcaca ttaatgttta cgcatcccag accaacaatc tggatacggc gaaactgatc
    10981 gcagagtctg caagcaccac ctttacacat gctggtcttg gggatagcga aacgtggtat
    11041 tactgggttc gagcatcgaa caagcgaggt atgttaagcc ctccgaactc aaacttgggt
    11101 acagaggcga caactcgcga tgtactgtcg ttcctggctg gcaaaattac atcttccgag
    11161 cttggtcagg ctctgcttga ggacatcaac agcaaagcct ctcaagaagc agttgacgag
    11221 ttaaatgagc atatcaacca gagcgtcgaa tctttggagg ggtcggtaaa cgacgttaag
    11281 gaagatatag ctgaattaga taagcagttc agcgataacc tcgcgggttt tgaaataaaa
    11341 ttcaacgagc gtagcgatgc tttggagaac gcacaaaccg agcttaaagg tgaggtttcg
    11401 gcaacgatcg acaaggtcaa tgaagcgttt gaaaaaattg atgctactga tgctgcaatt
    11461 gttgaaatcg aaaacaccgt atctgaacac gataaagctc tcgctaatac agtcgaggca
    11521 ataaaggctg caagagatga agcggcggct cttattgcta aggaaagcga ggctcgtgtt
    11581 gaaggcgatg ctgcaaacgc taaacaactg gaagtgttgc agtcaacggt agaggaaagc
    11641 tccgctgccg ttgaagaaat gaaaaagacg gttgcagagg tcgatcgtgc cagtgcagaa
    11701 ctgactacga acattgaggc gttagccaaa acaaatattg acctggctct tcgtcaagat
    11761 gaagaccagc acaagcagat ggtcaataac gcgaagatcg caacaacaca gaaaaccttt
    11821 gctgatgata tgtctgcaat ggccacgaag gttgaggaaa ttcgcgtaga aattggcgaa
    11881 gacattaagg ccagtattct ggaagagtcc accgctcgtg ccgatggcga tgaagcgttg
    11941 gcaaagcgtg tcactcagct gcaatctaag tttgaaggag atatcagcgc ggcgattagt
    12001 acagagcagg aggctcgcac atctgccgat gaagctctga cgacacagat cactcagctt
    12061 gagtcgaaag taagcaacga tatcgtcgca gctattaagg aagagcaaga agcccgagca
    12121 acggaagatt cggctctggc aagccagatc acccaacttc aggcaaaggt tgatgatgat
    12181 atttctgctg caatacgtag tgagcaggag gcccgagcaa gcggggactc tgcgttagcc
    12241 aaacaaataa accagcttca gtcaaaggtt gacggtgata tatccgctgc catcactcag
    12301 gagcaggagg ccagagcaag tgctgaccag gcgctctcac gagaaattaa cagcctgcga
    12361 gcacaaaccg gaactgatat tgccgcagcg gttgctgtag agacaaaggc aaggactgat
    12421 gccgatagtg cattgtccac tcagatttcg tctcttacag caaaagctaa tgatctcgag
    12481 gcatctcttg ccagagaaac aacggctcgt gctgatggtg ataccgcttt aacaaaagag
    12541 gtttcaagtt taaaagcaca gacagcgaaa gatattagtg cggccgttgc agttgaaacg
    12601 caggctcgaa ctgatgctga ctcggcgttg tcgtctcaga tcaccaagct gacctctcaa
    12661 tacaaagagg atattaaagc ggctgtagca accgaaacga aaacgcgtac agagcaagat
    12721 gctgcattgg cgactcaaat cacaaactta gagtctcaaa cggcagccaa catttccgcg
    12781 gcggtaacaa ctgaaacgac agcgagaacg caggcagata atgctctgag tgggcgaatc
    12841 gatactctga aagcagaggt ggatggaaat accgcgacaa ttcaacagca ggcaacagct
    12901 attgctgata ccaacaaaaa agtgtcaacc gcgtggacgt tgaaaatgga aacttcaacg
    12961 agcggagggc agaagtatgt tgctggtatt gcgcttggta tcgacaccac tggcctttct
    13021 cagtttttgg tgcaggctga caggtttggt ttggtcaact ctgttaacgg gaagatcacc
    13081 acgccatttg ttatcgaaaa cagtatcgcc tatatgaatg gtgcgtatat caaggatggc
    13141 acaatcacca atgcaaaagt aggcgatctg caatctacca attttgttag cggtagatct
    13201 ggatggcggt tcggcaaaaa tggaacgctt gagatcaacg gtaatagcgg gggtaatggg
    13261 cgactggtca taaatggtca gcggattgac gtttatgacg ataacaacgt cctacgagta
    13321 agaattggcc ttctgtaatg tagaaaaaaa tatttatatc ggtaaaaata agtgggtaca
    13381 tacttaatgg tgggcaagga tagcccacca aatcaaggag catattcaac atgtggtaca
    13441 gggaaggtac tatcacattt acacagggca gcaatactct ggttggggca gggacagcct
    13501 ggaacgtaac agctaatggt gtgttgccgg ggatgatcgt cattggcccc gacaataagc
    13561 tgtatgagat caagcgcgta accagtgata cgaacattgt tctctcagaa ccttataccg
    13621 gcgaaactca gtctgaagtt ccgtgccgaa tcattacgac ctatgaaggc gacttaacac
    13681 agtttagcgc gcgctttaca gcactaatgt cgcgtatgtc ggctgattcc aagtccatgc
    13741 gcagttggtt gactgctctg gatgaggtga caatcgagcg tgaagacggt acagaagtga
    13801 ccgttaagcc gctaatgcag atcgtcaacg agcacaacga aaacgttgag tggtataaaa
    13861 ataacactga cgcgattgat gctgctggcg acaaagctcg tgaggcggcg gccagtgctg
    13921 ccgcagcagc agaaagcgcc aataccgctg gagaaaaagc ctctcaagcg tctcaaagtg
    13981 catccgctgc agagtcctca aaaagcgcgg cggccaccag tgccggtgcc gcgaaaacgt
    14041 ctgaaacgaa tgctgcagcg tcacaaaaat ctgcagccac ttctgcatcc accgcgacca
    14101 cgaaggcgtc agaagctgcc acctcagccc gggatgcggc ggcctcaaaa gaggcagcga
    14161 aatcatcaga aacgagcgca gcctcgagcg ccagtaatgc cgcttcctcg gcaacggcgg
    14221 caggaaattc cgcgaaggcg gcaaaaacgt ccgagacgaa cgccaggtct tctgaaacgg
    14281 cagcgggaca gagcgcctcg gctgcggcag gctcaaaaac agcggctgcg tcgtctgcca
    14341 gtgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc ggaaaatcgg
    14401 cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa ggctggcgaa gccactgaac
    14461 aggccagcgc agcagcgagg tctgcttccg cagcgaagac atccgaaacg aacgcgaaag
    14521 cgtcggaaac ccgtgcagaa tcctcaaaaa cggcagccgc atcgtccgcc agttcggcag
    14581 cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacag gcgtcagcag
    14641 cgaagggcag cgccacgacg gcatccacga aagcaacaga ggcggcaggc agtgcgacgg
    14701 cagcatctca gagcaaaact gctgctgaat ccgctgcgac ccgtgcggaa gctgctgctg
    14761 atcgtgctga agagattgcc ggtgcagttg cgatggaaga cgcaagcctt acaactaaag
    14821 gtgttgtgaa acttagcagt gctgtcgaca gcaccagtga atcgctggcc gcaacgccaa
    14881 aagcagttaa agtagtcaat gacaatgcga acagcagggt gccatctaac cgaaaaatta
    14941 acgggaaagc actgactgcg gatatcacat taacgccgaa agatattggt actttaaatt
    15001 caataacgat gtctttctct ggcggggccg ggtggttcaa actggctact gttacaatgc
    15061 cacaagcgag ttccatcgtt tacatcgcat tgattggtgg cgctggttac aacgtcggct
    15121 ccccacatca ggcaggcatt tcagaactgg ttctacgagc aggcaatgga aaccccaaag
    15181 ggattacagg tgctttgtgg aagcgtacag ctgtcggatt aacgaatttc gcctggatca
    15241 acacatccgg cgatacatat gatatttacg ttgagattgg caattatgcg actcgtgtaa
    15301 atatccattg ggattgtact gcaaatgcga cagtttctat ttatacatcg ccaacatatt
    15361 cagctagtaa gccttccagc gttaccgatg gtgttgttta tacgatgtat agcacacatc
    15421 agaaaccgac gccgttagat attggagcac tgccaacaac cggaggaaca gtttcaggtc
    15481 cgttgtctgt tactggtggg atcaccggaa cattaaatgg taatgcaagt acagcaacga
    15541 aattgcagac ggcaagatct atcggtggag ttggtttcga cggttctgca aatatcaacc
    15601 ttccaggtgt aaatactacg ggtaatcaga acaccactgg taatgctgca actgctacaa
    15661 aacttcagac ggcaagaact atcggcggcg tgagctttga tggtactgcg aatattaatt
    15721 tgccaggtgt taatacgact ggtaatcaga atacaacggg caacgcggct actgctacga
    15781 agttgcagac tgcgcgtact atcaatgggg tgtcgtttga cggctcggca aatatttcct
    15841 tgtcgccagc aaatataggt tgcccggcat ctcctactgg ttggttaact acaggaagta
    15901 atggcggagc aataacaaca gcacagttag tgacgttatt gcaaaataat ggagcattta
    15961 acacaaagtc atggattgct cgatgtgctt gggcctatgc caatagtgca accataccaa
    16021 atagtgaaac tggttgtggc gttattccat tggcaggagc tgttatagag gtatttaata
    16081 acggtagtag ctcaaacaat tatacgatcc gtataacaac ggccacaacg acgagtgtct
    16141 ctggtgctct cactaatgcg gagtttatct atgtatttaa tggcacagat tattctccgg
    16201 gatggcgaag agtatataac acgaaaaaca aaccaacagc ctctgatgtc ggtgcattac
    16261 ctcttaccgg tggtacatta tctggaggtt tgacatcttc tggcgagatc atttcaaaat
    16321 atgcaaatgg tttccgcatt gcttacggta gctttgggtt ctttatccgt aatgatggat
    16381 cgaacacata tttcatgcta acagcatcag gagacacatt aggttcatgg aacggtttgc
    16441 gacctattac aattaataat accagcggtg ctgtatcaat tggtaatgga ctaaatgtga
    16501 ctggtggcgt aaatggtagt ttgaacggta atgcttcaac agctacgaag ttgcaaacag
    16561 cgagaaacat caatggtgtt aagtttgatg gctcaggcga tatcaacatt aatacattgg
    16621 tatctcgtgg ccgagttacg gcgttaagcg gctctactca aggcactgct ggcattcaaa
    16681 tgtacgaggc gtacaacaat agctacccga ccatgtatgg caacgtattg cacatgaaag
    16741 gtgcgagtgc tgctggtgag ggagagttgc ttattggctg gagtggtacg agcggtgcac
    16801 atgcgccagt tttcattcgc tcacgaagag ataacacagg tgcggcatgg tcagcgtggg
    16861 cgcaggtata taccgctaag gattcaatcc ctggtgtgaa tacaactggt aatcagaaca
    16921 ctactggtaa tgccgcaacc gccacaaaat tgcagacagc aaggaaaatt gctggtgtgg
    16981 cgtttgatgg ctctgccgat ataactttga ctgcggctaa ccttaatgct tacacgaaaa
    17041 cagaggtaac aaaccttcta agttcctatg caagcagatc atcactgaca ggctatagtg
    17101 gcaacctgga tattattgct gaaacactgg ttgtcaaatc aggcggtagt ggagggtttg
    17161 ctatatggga tattggcaca actactagcg gtgccaatat gtacattgat ccaaacccag
    17221 gtatcaatac agtttggcgt tcaacctctt caaggcgcta taaaaaggat attgaaacat
    17281 tacaagatcg atatgctgat gaacttttgt cattaagacc tgtttggtat cgttcaattt
    17341 gtcgaggtga ccgaaaggat tgggggtatt acggccttat tgctgaagag gttggtgaga
    17401 ttgccccgca atatgtccat tggcgtgaac caacaaataa tgattctcca gaagatattt
    17461 cctcaaatgg tatggtcgct gaaggagtga tgtatgagcg tttggttgta ccactcattc
    17521 atcatattca gcaattgacc aaaagggttg aggagcttga aacgaagtta aattcaccta
    17581 aagaataaac aacttcaatc ggggctggaa ctaaaccagc ccctgcttaa aagtataagg
    17641 atatatttat gagctatggt gcacaggttt ggtcgccatc aagacaggag atggtcgatg
    17701 cgttggcccc tgtttattac ctcgattatt ttactccctc tggttcagga agtagaacat
    17761 atgaggttga ggcgggattg ggaatcgact attacataat ggacgtaacc aatggaaaat
    17821 acacaagtct tacggtttct ggaaatacca tttcgtggtc tggagctagt ggtaatttaa
    17881 acatattggt ttttcaaaaa taatgtacgg aagcaagata tatcgatctg acgggaaggt
    17941 ctggatgtct ccttctttgt ctcctatcgt ttttcagaga aagcaggttg tttctttgtc
    18001 cggcgggaca gagtttaaca cccaaatatc tccagatcgt tcaccgatga tttttgttgc
    18061 ctattcgaaa gctgtttcat taatagccaa cagaatagtg cgtaacaacc aggtgattta
    18121 cagtttcgga ggtcagggta gcgactctag tgccacgata tacgtatttt cgaaaggtat
    18181 agccaaaaaa gaaacttggg gcatgagttt tttcaatgca caaggggagg aaatctataa
    18241 tacggcaaat atcccacttt catttacctt tcttaataat acggagtgga acagctctgg
    18301 agggcatgtt tttgattacc caccagcgat aatcccaaca tatgcgaatg tgttcgctgt
    18361 tccagtaccg ggcggagcca tgacaatggt ttatggatat gcggcatatg gaaatactgt
    18421 tagctcgata ttcgtaaacc aacttaatgg cggccatagt tttagcgtaa atggcagagt
    18481 accggtaata aatagaaatc tgtataactg atgagtaaat gatgaaaaga ttatttctaa
    18541 caatagctgt tgttctctct cttactggct gccagaaatt gccgacacct atatgttacg
    18601 gtgaggctat cattggtgga caggagacag tcatacccat atatgctgtt aaaaaagtga
    18661 acgactatac cttatacaaa gcgggtagca tctataactg gcgatgggta ggagtaggtg
    18721 ctttctcatc agtccgttgc aatgctgaat gataggtaag tacacaccta caacaaaagg
    18781 gtgaaatgtg atataaatcc gccatcccga tttgactttt catggaggaa aacatgtcga
    18841 acgagatggc gggcgttaca acagagcaag ttgagcgtat tgccgcgatc gttgctcggg
    18901 aggttgttgg caaattaggc aaagagctac gtgaagaaat tggccaggag gtcaacgatc
    18961 agctgaaaac ctactttggt gatatgaccc cggcgcaaca tagtattcaa cactccaacc
    19021 tggacaaact ccttaaccgg ttagattcca tctcaagtgg gttctttggc ggcattgttt
    19081 ctaaaataac gtcgttcatt attaccgcac tgcttttggg gttagccgcg tatggcgtaa
    19141 aaaatggact gcaataacag gagatcaagg atgaagactc cgagaggtat tcgtaataat
    19201 aaccccggta atcttgataa aggatcaccg tggcaaggtc tggttgcgaa tccagacgaa
    19261 ccgcgctttt gcacgtttaa agaccctgtt tgggggattc gtgcactggc ggtgactcta
    19321 attacctacc acgacaagcg tcgcgcaaaa gacggctcaa gtatcgatac cattcgtgaa
    19381 gttattgaac gttgggcacc gccgaatgaa aacaacactg acgcctacat taatgaggtg
    19441 tctaaagccg ttggtgtaac cgcagacatg attatcgatc tgcatgatta cgacattctt
    19501 cgacctttgg ttgaggcaat cattcgccat gagaatgggc gaggtccgct aaaaacgctg
    19561 aacacctggt atgcggcaga agttattgag gaaggtctgc gtcgagctgg cgtcgttaag
    19621 ccggtgaaaa ccgtgaaggc tgttcctgta actaaagaaa cagcaggcgc aactgttaca
    19681 gcaggtattg gtctggcgca gctggccgat gttatgccgc aggtttccgc tgctatggat
    19741 aaagcacaag gtcatatctc tagcggggat acagtacgca tcatcttcgg tattgccact
    19801 attgttgtgg ctggatttat cgcctggtcg caggtaagaa gacaccagaa agggatggtc
    19861 taatatgcta ggcagcctga tgacaaagct aaaagttgct ttgattacgc tggctgccgt
    19921 tcttttcgtt ctggtcggcg cttacacgat gggcggaaat gcggcgcgac gagcaatgga
    19981 agagaaggca aaacaggaag acagaaaacg acttcaaagc acagtggatg tcaaaaatga
    20041 aacggttaat gaagtgcggc aaaaagatgc ctctggtgtt catcgcgagc tgtttgataa
    20101 atgggtgcgt gattaagacg caggctccag gcgtgctatt ctgcgatgct gcaaatccta
    20161 tctatgtgag tagagaggat tttatgactg aggaaacgga gcgacagttg cttatccaca
    20221 atacgatagg tgaaaggctg tgtgagtggg ggcacctgaa ctgagtcgat gattgatgag
    20281 tggtctcagt ttacctttta tcctaaagat ggcatcttca ctcatataca cgcctgttca
    20341 ctcaagctca tgttgaatca tttagtaatt tgatgtagat ttattaggca tccacgtaag
    20401 tgggcacata caggctacgc atcctcgtag ccttcaaccc cgagaggggt gccgcaatga
    20461 cacttgtgtt aacggctaag gtggatatat gctaaattat catgacaata cgcgttctat
    20521 gcagacgatc cgcaccaata ctgcggttat cgacagcttt cctatgcgta ttcacaacag
    20581 tgaagacgca gtagaagtgc gacgtatgtt gtgccgtgaa tcagctaacc gccagcactt
    20641 catcgtcact tttaagagcg atgtggcgcg tgccgaaaag atctctaatt ctacgtcact
    20701 ggttaaaccg ttggccgagg ttgtcgctaa cggtaaacga acctcttttg ttttagatgt
    20761 tgctgaacaa tatcctgact tgaagtgttt agatcgtgat atttcttcac gaatcaaaca
    20821 agttgtagag tcttttatga agaaattctg taaaatcgat gatatggaac catcaacggc
    20881 acagaatact tcatggcacc atctctaatt ggtcaggcga acagattaat ccctgaaata
    20941 gaagactaca gcaaggtctt gtctgaaatg ccgagagcga cgagaattat cgctctcggt
    21001 gagttctatt ttatctcccg aggtttcgat ctaattcgat ttaccgaata cgagcttgtt
    21061 gattcggcct atgaacgctg tattgttggc gagtgtcttg agtcatgtga tcctgattac
    21121 caagagcagg aagttaagcg actcaaacgt gctggttaca cacctcttga gataggagca
    21181 gaaattgaat cctacaagtt gcatgtgggg cagccagtcc aaacagtctt ccgcgtggcc
    21241 tactgcgagt ttccaatcaa ggataaatct ggagtaaaag tcagcgccaa gcagattaga
    21301 ggggttttta ctcatgctga ttatatggga ttaggtatcg caagttttgc ttacctttat
    21361 ctcgtagata agtatcagta cttggtttgc gatacgttac agacggtaaa aggcgcaact
    21421 ctttgggctg tgagtatgcg caaacatgga ctggtcgaaa tttacgatac gaagaacgaa
    21481 aattttatag aggaattggg tgaggatgcc tgtggcgtaa gtggttttat tccgtgggat
    21541 atcggtaagt ctgacggagg ccagatgcga agacgtgaag agtgggggac taataaaatc
    21601 aatctcaccc caggtgcttg tactcatata gtgaacattc tgactaaacg atctatgaat
    21661 caataaaccc gctacggcgg gttttttgtt attaagcccc cccctatgta cctatcatta
    21721 aacctttaca ccgcagccgt aggcatttag gctatatcac atataagaaa acaagttgtt
    21781 tcagacgata attatatacg caaagggaac tctccaatga ccaagatctt tgtggttggc
    21841 ggcacaaagg gcgggcctgg caaatccacc gttgcccagc aaattgccgt ttgcctgaaa
    21901 gtcaaaaaga agaagaaggt ttatattacc gatatagata ttcagcgcac gacaacgagc
    21961 tggtgtgaag accgtcgaca gaacgaagac cttgagctga ttccttttgc atacgttcag
    22021 gatgacatca ttaagcacct aaaatcgctt cagggtagag ctgagtttgt agtggtagat
    22081 gctggtggct tcgactccga aattcagcga caagcgatgc tgatggctga cgttatcatt
    22141 atcccgctgc gtcctaagcg tcgtgatttg aaatctttac gtgacatcga tcctattatc
    22201 gacaatgttc gcaatgtaaa cgataaagtg aaggtccgcg cggtcatgaa ccagtgcccg
    22261 gctttgccat cacaagtgtc tcgcattctg gcggctaaag agattgttga gacgtttggc
    22321 atcgagtctg cgccagtcaa tctatataac cgcaacgtct atgatgatgc ggaagagtct
    22381 ggtcgttcta tctttgaaat gaccggtagc gagcgcgaca aaaaggcgga agccgagttt
    22441 gaagaatttg tagattatct gttgagtctg gaggaagaag aataatgtcc atgaaaatgg
    22501 gtgacctagc aaagcgcaaa gagcctgatg caccggctaa gaacacaact cctttgcgcc
    22561 aaccagtcag accacaggga cgcccgactc gtggcaaaga gaaaattaaa agccgcacaa
    22621 tgtcactgga ggacgaatac ttcgaactgc tggagatgat gaagttcatc cctcgcttcg
    22681 agaagttcac tcgttctgac gtgattcgtg ctgctatttt ccatcttgcg gagaagacac
    22741 cgcaggaaat cgaggacatc gtgaaactta acgaggcgat cacagctgcc gatgtcacga
    22801 tgcgtaccga tgaaatcaaa cgcgagttga tgaagaaagg ttaattttat tgaggtgctg
    22861 cttggcacct ctttctatag gttccctaat gctctacaaa tatattggaa gtgaagattc
    22921 gcaaaaagtg ttacgtaacc tcaaacggtt tgtagaggac ggcactatct ctgctagcgc
    22981 accttcttca ttcaacgacc catcagagtt caaagtttcg ataacgatgg gcggtacaga
    23041 agtggagcgc cggaaacact ttgaagacaa tggatgtaag gatttttcct ttgaagactg
    23101 ggatgctgta gctcctgaag ctgcaaaatc gttggctgtg gatttgcgga aagaatcatt
    23161 aggggctttt ggtgtggtgt gcttgtcacc aaacgaagat aacatcttga tgtggtcaca
    23221 ttactcagtg tcgcataaag gtttctgcat aggctttgat gatgattttg ttacctctat
    23281 tgaggggtat cacagctttg ataatgtgaa atatatacct tctgttcccg tgttcgatga
    23341 agttacagat gagtttaagg tactttttga taagctcttt ttctaca
//
LOCUS       DAPQZD010000068        22951 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPQZD010000068 DAPQZD010000000
VERSION     DAPQZD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.068"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            148..8664
                     /locus_tag="RO454_002579"
     CDS             148..8664
                     /locus_tag="RO454_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HDY0934396.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     DSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDGILDVSGGGHGIDITGDSAT
                     VDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNNGNTTV
                     DGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSAKVDNKGGMTVTDPDSIGIL
                     IDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNGNTTVDGQGSTGTEIAGNNAVVN
                     QDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISN
                     GGTGTQVNGDEATVNNNGNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITG
                     DSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNG
                     NTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDS
                     IGILIDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNGNTTVDGQGSTGTEIAGNN
                     VVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDN
                     AISNGGTGTQVNGDEATVNNNGNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGI
                     DITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATV
                     NNNGKTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVT
                     DPDSIGILIDGDKAIVNNDGDNAISNGGTGTQINGDDATANNNGKTIVDGKDSTGTEI
                     AGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNAISNGGTGTQVNGDEATVNNNGKTT
                     VDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGI
                     LIDGDKAIVNNDGDNAISNGGTGTQINGDDATANNSGKTIVDGKDSTGTEIAGNNAVV
                     NQDGTLDVSGGGHGIDITGDSAKVDNKGGMTVTDPDSIGIQIDGDKAIVNNDGDNAIS
                     NGGTGTQINGDEATVNNNGNTTVDGKDATGTEIAGNNAVVNQDGTLDVSGGAHGIDIT
                     GDGAIANTTGAITVADTDSVGIQVDGNAVTVSNDGDSTITNGGTGTQVNGNDAQVNNT
                     GNTTVDGKNSTGTEITGNNGTVNLDGGLIVTGGAYGVEIAGDSAALNNEGDISVSDEG
                     SIGVLINGEKATVSNTGDVNVSNNATGFDITTNEGNISLAGSMQVGDFSTGMDLNGNN
                     NSVTLAAKDLNVTGQKATGVNVSGDANTINITGNVLVDKDQTADNAADYFYDPSVGIN
                     VNGSDNNVTLDGKLTVVADSELTTRGSAGAIIFDGSQENITGLSIAGDGNTFRLNGGI
                     QLVGEANKLTDGSTIASERKGSGKVPLVSVDGKSSVHLNGDSTISGDVLLGNTQIIDL
                     RNGAMLDVGADATINMQVDTYERYAMSADHLIDASSGSQLVNNGDINLRSIGFAAITG
                     EDSFGSNTGNLTLSQYLDGLFANGGYGYLATKGASVVNTGTITTKVAEQESVINLGAS
                     LGFNDLVFYNAANSMTGLSAKTHGYVLNENGGKIEMFGRGNVGMLAIDESTAENAGQI
                     TLDALWVDVNDTTTLRDNIGNDARGYSVGMGVGTDAGSGPRKNATAVNQQSGVITVYN
                     AGIGMAAYGAGNTVINQGTINLEKNGNYDSSLGVNSLIGMAAYNSGTAINDQTGVINI
                     NADNGQAFYSTGNGVILNYGTICVNTNCLTGNDYNDTDSYTSILYTGGDVITAQSETQ
                     SLTKKATISDKKDGNVVNSGALSGADIAISSGKLVNTSTGTINNAIIINGGALSNEGS
                     VAKVTQNAGTFNNTGSVNSRMTQTGGTFNNQQGGTVKNGSRLANSAIANNMGIWSLGD
                     TSSGSNASMLEINNNAVFKNSGDFILDNSKNAVHLNQSGTFYNTGKMLLSNSSHSGVL
                     NFWGGNGRFINGGIADVTAKSLAVSASDAGSSNAFFWNQSNGVVNFDKDSGVAVKFIH
                     SNYVAQNDGTMNISGNNAIAMEGDKNARLVNNGTINLGTSGTTDTGMIGMQLDSGTTA
                     DAVIENNGAINIYANNSFAFSMLGSVGHLVNNGTVTIADGVTGSGLIKQGDSVNIEGV
                     NGNNGNNSEVHYADYTLPDVPGSSVSVAASTPSSDGSQNKLNGYVVGTSSDGSAGKLK
                     VNNASLKGVSVNTGFTAGTSSTSVTFDNVVQGSNLTDAETITSTSVVWNAQGTTDASG
                     NVDVTMTKNAYTDVATDSSVNTVAQVLDAGYTNNELYSSLNVGTTAELNSALKQVSGS
                     QATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNDTQYDMMALRKS
                     LTLTEHQNLSLEYGIARLEGNGSDTAGDNGVTGGYSQFFGLKHQMAFDNGMNWNNALR
                     YDVHQLDSSRSVAYGDINKTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRH
                     TLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKDGWSANATLEGGPNLSYVK
                     SQRTASVSGAGSQRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAFHWKEDGISDK
                     GVMLNFKKTF"
     gene            8884..9750
                     /gene="pdxI"
                     /locus_tag="RO454_002580"
     CDS             8884..9750
                     /gene="pdxI"
                     /locus_tag="RO454_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDY0934397.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(9790..10395)
                     /gene="azoR"
                     /locus_tag="RO454_002581"
     CDS             complement(9790..10395)
                     /gene="azoR"
                     /locus_tag="RO454_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDY0934398.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            10596..14498
                     /gene="hrpA"
                     /locus_tag="RO454_002582"
     CDS             10596..14498
                     /gene="hrpA"
                     /locus_tag="RO454_002582"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDY0934399.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            14771..15571
                     /locus_tag="RO454_002583"
     CDS             14771..15571
                     /locus_tag="RO454_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDY0934400.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            15781..17220
                     /gene="aldA"
                     /locus_tag="RO454_002584"
     CDS             15781..17220
                     /gene="aldA"
                     /locus_tag="RO454_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDY0934401.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(17262..18263)
                     /gene="gap"
                     /locus_tag="RO454_002585"
     CDS             complement(17262..18263)
                     /gene="gap"
                     /locus_tag="RO454_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDY0934402.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            18452..18982
                     /gene="cybB"
                     /locus_tag="RO454_002586"
     CDS             18452..18982
                     /gene="cybB"
                     /locus_tag="RO454_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDY0934403.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            19227..19400
                     /locus_tag="RO454_002587"
     CDS             19227..19400
                     /locus_tag="RO454_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934404.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(19472..19621)
                     /gene="hokB"
                     /locus_tag="RO454_002588"
     CDS             complement(19472..19621)
                     /gene="hokB"
                     /locus_tag="RO454_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDY0934405.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            19884..20327
                     /gene="trg"
                     /locus_tag="RO454_002589"
                     /pseudo
     CDS             19884..20327
                     /gene="trg"
                     /locus_tag="RO454_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(20365..21288)
                     /locus_tag="RO454_002590"
     CDS             complement(20365..21288)
                     /locus_tag="RO454_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0934406.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            21505..22848
                     /locus_tag="RO454_002591"
     CDS             21505..22848
                     /locus_tag="RO454_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDY0934407.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHKEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         5986 a         5488 c         6206 g         5271 t
ORIGIN      
        1 caaattataa ttacagattg tcagagaata tgtttgatta ggtgccaatc tactggcaat
       61 attgaatgtc ctctatgttt taattgattg attaattggt tgggttaagg catccgacct
      121 tctttatatt cgccagaagg atttattatg caaaggaaaa ctctattgtc agcctgtatt
      181 gcattggctc tgagtggtca gggctgggcg gcagatatca cagagataga aaccaccaca
      241 ggtgaaaaga aaaataccaa tgtgacttgt ccggcagacc ctggaaaact cagtccagaa
      301 gagcttaaac gcttaccctc tgaatgctcc cctgtagtcg aacagaacct gatgccatgg
      361 cttgccacag gtgctgctac tgcgttaatc acagccttag ccatagtgga actaaacgac
      421 gatgatgatc atcatcatcg caataattct ccacttccac caactccacc tgatgataat
      481 tcagacgaca ctccagttcc cccaactcct ggcggagatg agataatacc agacgacggt
      541 tcggatgata ccccgacgcc tcccaaacca atttcgttta ataatgacgt tatcctcgat
      601 aaaacagcaa aaacgttaac tattcgcgat tcagttttta cttataccga gaatgctgac
      661 gggactattt ctctacaaga tagcaatggc cgtaaggcaa ctattaatat ttggcaaatt
      721 gatgaagcga ataacacggt tgcccttgac ggggtgagcg cagatggcgc aacgaagtgg
      781 caatataatc ataacggtga gcttgttatt acgggtgata atgccacagt aaacaacaat
      841 ggcaaaacca tcgttgatgg taaagattcc actggcacgg agatcgccgg taataacggg
      901 aaagttattc aggacggtat actggatgtc agcggcggcg gtcacgggat tgatatcacc
      961 ggcgacagcg ccacggtgga caacaagggc ggtatgacgg ttaccgatcc ggattccatc
     1021 ggtattctga tcgacggtga taaagccatc gtcaacaatg acggcgacaa cgccatcagc
     1081 aacggcggca ccggcacaca ggttaacggt gatgaagcca ccgtgaacaa taacggtaac
     1141 accaccgttg atggtcaggg ctctacaggt accgaaatcg caggtaataa cgcggtggtg
     1201 aatcaggacg gtacactgga cgtcagcggc ggcggtcacg gtattgatat caccggcgac
     1261 agcgccaaag tggataacaa aggcggtatg acggttaccg atccggattc catcggtatt
     1321 ctgatcgacg gcgacaaagc catcgtcaac aacgacggcg acaatgccat cagtaacggc
     1381 ggcaccggca cgcagattaa cggtgatgaa gccaccgtga acaataacgg taacaccacc
     1441 gttgatggtc agggctctac aggtaccgaa atcgcaggta ataacgctgt agtgaatcag
     1501 gacggcacgc tggatgtcag cggcggcggt cacgggattg atatcaccgg cgacagcgcc
     1561 acggtggaca acaagggcgg tatgacggtt accgatccgg attccatcgg tattctgatc
     1621 gacggtgata aagccatcgt caacaatgac ggcgacaacg ccatcagcaa cggcggcacc
     1681 ggcacgcagg ttaatggcga tgaagccact gtaaacaaca acggcaacac caccgttgat
     1741 ggtcagggct ctacaggtac cgaaatcgcg ggtaacaacg cggtggtgaa tcaggacggt
     1801 acactggatg tcagcggcgg cggtcacggt attgatatca ccggcgacag cgccacggtg
     1861 gacaacaagg gcggtatgac ggttaccgat ccggattcca ttggtattct gatcgacggc
     1921 gacaaagcca tcgtcaacaa cgacggcgac aatgccatca gtaacggcgg caccggcacg
     1981 cagattaacg gtgatgaagc caccgtgaac aataacggta acaccaccgt tgatggtcag
     2041 ggctctacag gtaccgaaat cgcaggtaat aacgctgtag tgaatcagga cggcacgctg
     2101 gatgtcagcg gcggcggtca cggaattgat atcaccggcg acagcgccac ggtggacaac
     2161 aagggcggta tgacggttac cgatccggat tccattggta ttctgatcga cggtgataaa
     2221 gccatcgtca acaacgacgg cgacaatgcc atcagcaacg gcggcaccgg cacgcagatt
     2281 aatggcgatg aagccactgt aaacaataac ggtaacacca ccgttgatgg tcagggctct
     2341 acaggtaccg aaatcgcggg taacaacgtg gtggtgaatc aggacggtac gctggatgtc
     2401 agcggcggcg gtcacggtat tgatatcacc ggcgacagcg ccacggtgga caacaagggc
     2461 ggtatgacgg ttaccgatcc ggattccatc ggtattctga tcgacggcga caaagccatc
     2521 gtcaacaatg acggcgataa cgccatcagc aacggcggca ccggcacgca ggttaacggt
     2581 gatgaagcca ctgtaaacaa taacggcaac accaccgttg atggtcaggg ctcgaccggt
     2641 accgaaatcg cgggtaataa cgctgtagtg aatcaggacg gcacgctgga tgtcagcggc
     2701 ggcggtcacg ggattgatat caccggcgac agcgccacgg tggataacaa aggcggtatg
     2761 acggttaccg atccggactc catcggtatt ctgatcgacg gcgacaaagc catcgtcaac
     2821 aacgacggcg acaacgccat cagcaacggc ggcaccggca cgcaggttaa cggtgatgaa
     2881 gccaccgtga acaataacgg taaaaccacc gttgatggtc agggctcgac cggcacggaa
     2941 atcgcgggta ataacgctgt agtgaatcag gacggcacgc tggacgtcag cggcggcggt
     3001 cacgggattg atatcaccgg cgacagcgcc acggtggaca acaagggcgg tatgacggtt
     3061 accgatccgg attccatcgg tattctgatc gacggtgata aagccatcgt caacaatgac
     3121 ggcgacaacg ccatcagcaa cggcggcacc ggcacacaaa ttaacggtga cgatgcgacc
     3181 gcgaacaaca acggcaaaac cattgtcgac ggcaaagatt ccaccggcac ggaaatcgcg
     3241 ggtaataacg ctgtagtgaa tcaggacggt acgctggacg tcagcggcgg cggtcacggt
     3301 attgatatca ccggcgacag cgccacggtg gacaacgcca tcagtaacgg cggcaccggc
     3361 acgcaggtta acggtgatga agccaccgta aacaataacg gtaaaaccac cgttgatggt
     3421 cagggctcga ccggcacgga aatcgcgggt aacaacgcgg tggtgaatca ggacggtacg
     3481 ctggacgtca gcggcggcgg tcacggtatt gatatcaccg gcgacagcgc cacggtggac
     3541 aacaagggcg gtatgacggt taccgatccg gattccatcg gtattctgat cgacggtgat
     3601 aaagccatcg tcaacaacga cggcgacaac gccatcagca acggcggtac cggcacacaa
     3661 atcaacggtg acgacgcgac cgcgaacaac agcggcaaaa ccattgtcga cggcaaagat
     3721 tccaccggta ccgaaatcgc gggcaacaat gcggtggtga atcaggacgg cacgctggat
     3781 gtcagcggcg gcggtcacgg tattgatatc accggcgaca gcgccaaagt ggataacaaa
     3841 ggcggtatga cggttaccga tccggattcc atcggtatcc agatcgacgg tgataaagcc
     3901 atcgtcaaca acgatggcga caatgctatc agcaacggcg gcaccggcac gcaaattaac
     3961 ggcgatgaag ccaccgtgaa taataacggt aacaccactg ttgatggcaa agacgccacc
     4021 ggcacggaaa tcgcgggtaa caacgcggtg gtgaatcagg acggcacgct ggatgtcagc
     4081 ggcggtgctc acgggattga tatcactgga gacggcgcca ttgcaaacac gacaggagcg
     4141 atcactgtag cggatacgga ctctgtcggt attcaggttg atggtaatgc agtgaccgtc
     4201 agcaatgacg gagacagcac cattactaac ggcggtacgg gcacgcaggt taacggcaac
     4261 gatgctcagg tcaacaatac tggcaacacc acggtagacg gcaaaaactc caccggaaca
     4321 gaaattacgg gcaataacgg cactgtaaat ctggatggtg gtctgattgt gacgggtggt
     4381 gcctatggcg tggagatcgc tggcgacagc gccgcgctaa acaatgaagg agatatttcg
     4441 gtttccgatg agggctcaat tggcgtactc atcaacggtg aaaaagcaac cgtatccaac
     4501 acgggtgatg ttaacgtcag caataacgcg acaggttttg acattaccac taatgaaggg
     4561 aatatttcgc tggcaggcag tatgcaggtg ggcgacttct ctaccgggat ggatctaaac
     4621 ggtaataaca acagcgtcac gctggcggca aaagatctga acgtcaccgg tcagaaggcg
     4681 acaggcgtga atgtatccgg cgatgccaac actattaata tcaccggtaa tgttctggta
     4741 gataaagatc aaacggctga taacgcggca gattatttct acgacccgtc tgtggggatt
     4801 aatgtcaacg gtagtgataa caatgtcact ctggacggta agttaacggt tgttgctgac
     4861 agtgaactta ctacacgcgg ttctgctggc gccattatat ttgatggcag ccaggagaat
     4921 atcaccggac tcagcattgc aggtgatggt aatactttcc gtctgaatgg cggtattcaa
     4981 ctggttggtg aggcgaacaa gctaactgac ggttccacta ttgcatccga gcggaaaggc
     5041 agtggaaagg tacctcttgt cagtgttgat ggaaaatcgt ccgttcatct gaacggggat
     5101 tccactatta gcggtgatgt gctgctcggc aatacacaaa tcattgattt gcggaatggt
     5161 gccatgctgg acgtcggtgc ggatgccacg atcaacatgc aggttgatac ttacgagcgt
     5221 tatgctatgt cagccgatca cctcattgat gcatcgtctg gctctcagtt ggtcaataac
     5281 ggtgatatta atctccgaag catcggtttt gccgctatta ctggtgagga ttcattcgga
     5341 agcaacactg gcaatctcac gctttctcag tacctcgatg ggctgtttgc aaacggaggg
     5401 tatggctatt tagccaccaa aggggccagt gtcgtgaaca caggcactat cactacgaaa
     5461 gtggcggaac aagagagcgt aatcaacctt ggagcgtcac tgggttttaa tgatttagtc
     5521 ttttataacg cagcgaacag tatgacgggc cttagcgcca aaactcatgg ttatgtactt
     5581 aatgagaacg gcggcaaaat cgagatgttc ggacgtggca atgtcggtat gttggccatc
     5641 gatgaaagta cggcagaaaa cgcggggcag attacactgg atgcactgtg ggttgatgta
     5701 aacgatacaa caactttgcg tgacaacatt ggtaacgacg ccagaggata ttctgttggt
     5761 atgggcgttg ggacagatgc tggcagtgga ccgaggaaga acgccacagc agtcaaccaa
     5821 cagagtggtg ttatcaccgt ttataacgcc ggaattggta tggccgccta cggcgctggt
     5881 aatacggtta ttaaccaggg caccattaat ctggagaaaa acggtaatta cgatagtagc
     5941 ctgggcgtga attcgcttat cggtatggct gcatataaca gtggtaccgc cattaacgat
     6001 caaaccggtg ttatcaatat taatgctgac aatgggcagg cattttatag cactggtaac
     6061 ggtgtcattc ttaactatgg caccatttgt gttaacacta attgcctgac gggcaatgac
     6121 tataatgata ctgactccta tacctcgata ttatacacgg gtggcgatgt catcactgca
     6181 cagagtgaaa cacaaagtct gaccaaaaaa gccacgatta gcgacaaaaa agacggtaat
     6241 gttgttaaca gtggtgcgct ttccggtgct gatattgcga ttagtagcgg taagttagtt
     6301 aatacatcaa cgggtacgat taataacgca ataattatca acggcggtgc gttaagtaac
     6361 gaaggctctg tggcgaaggt gacgcaaaac gccggtactt tcaataacac agggtcggtt
     6421 aacagtcgta tgactcagac aggaggaacg ttcaataacc aacagggcgg gacggtgaag
     6481 aacggctcca gactggccaa cagcgctatt gctaataaca tggggatctg gtcccttggc
     6541 gatacgagta gtggtagtaa tgccagcatg ctggaaatta ataataatgc cgtgttcaaa
     6601 aacagtggtg acttcattct ggataatagc aaaaacgctg ttcatctgaa tcagtcaggt
     6661 acgttctaca ataccgggaa aatgttgcta tccaactctt cccatagcgg cgtactaaat
     6721 ttctggggtg ggaatgggcg ctttattaac ggcggtattg cggatgtcac ggcgaaatct
     6781 ttggccgtaa gcgccagtga tgccgggagc agcaatgcct tcttctggaa ccagagcaat
     6841 ggggtcgtca attttgataa agacagcggc gttgcagtga aattcattca cagcaattac
     6901 gttgcgcaga atgacggcac catgaatatt agcggtaata acgctattgc gatggaaggg
     6961 gataaaaacg cacgactggt taacaacggc acgatcaacc tcggaacgtc gggcacgacc
     7021 gacacgggca tgatcgggat gcaactggac agcggcacga cagctgatgc ggtcattgag
     7081 aacaatggcg ccatcaacat ttacgccaac aactcctttg catttagcat gttgggttcc
     7141 gtaggccacc tcgtgaacaa cggcacagtg actattgccg atggagtgac aggttccggg
     7201 ctaatcaagc aaggtgacag cgttaacatt gaaggtgtga acggcaataa cggtaacaat
     7261 agcgaagtgc attatgctga ttacactttg cctgatgtac ctggctcgtc ggtttctgtt
     7321 gcggccagca ccccgtccag tgacggaagc cagaacaaac tgaatggtta tgttgtagga
     7381 accagttctg acggcagtgc cggcaagctg aaagtgaaca atgcaagcct gaaaggggtt
     7441 tctgttaaca ccggatttac ggctggaacc agctccacct ccgtgacctt cgacaacgtc
     7501 gttcagggca gtaatctgac cgacgcagag acgattacat cgacctctgt ggtatggaac
     7561 gcgcaaggca ctactgacgc cagcggcaac gttgatgtca cgatgactaa aaatgcctac
     7621 accgatgtag caacagattc atctgttaac accgtcgcgc aggtactgga tgcgggctat
     7681 accaacaacg agttgtacag cagcctgaac gtaggcacca cggcggaact gaacagtgcg
     7741 ctgaaacagg tcagcggcag ccaggcaaca acggtattca atgaagcacg agtgttaagt
     7801 aaccgcttca gcatgttgtc cgatgcagca ccggaagtcg caaacggtct ggccttcaac
     7861 gtggtagcga aaggcgaccc gcgtgctgaa ctgggtaacg acacacagta cgacatgatg
     7921 gcgctgcgta aatctctgac gctgactgaa catcagaacc tgagtctgga atacgggatt
     7981 gcgcgtctgg agggtaatgg ttcagatacc gcaggcgata acggagtgac aggcggttac
     8041 agccagttct tcggtctgaa acaccagatg gcctttgaca acggtatgaa ctggaataac
     8101 gccctgcgtt atgatgtgca ccagctcgac agcagccgct cggtagcgta cggcgatatc
     8161 aataaaacag ccgatgctaa tgtgaaacaa cagtacctgg agttccgtag tgaaggggcg
     8221 aaaacctttg agctgcgtga agggctgaac gtcacgccat atgccggggt taaactgcgt
     8281 cacacactgg agggtggcta tcaggaacgc aatgcgggtg actttaacct gagcatgaac
     8341 agcggtagcg aaacggcagt ggacagcatt gttgggctga aactggacta tgccggtaag
     8401 gacggctgga gtgcgaacgc cacgctggaa ggcggcccga acctgagcta tgtgaaaagc
     8461 cagcgaactg cgagcgtttc gggcgcggga agccagcgtt tcaacattga tgatggtcag
     8521 aatggtggtg gtttcaacag cctggcgaca atgggtgtga aatacagcag ccaggaaagt
     8581 gcgttgcagg tagatgcatt ccactggaaa gaagacggca tcagtgataa aggtgtgatg
     8641 ctaaacttca agaaaacgtt ctaattttta gcatgtaatg cataaaccgc aacgctgata
     8701 caggttgcgg tttttttatt gccggatgcg gtgcttgacg tgtcttaacc tgcctggggt
     8761 ttcgtgtctt tcagggcaac agtacaactc atcccgcaat cttgtttaca ccctcgcatt
     8821 atttatctct tttcgtttct atactgattt ttcttaatcc gttttattac agggcagggt
     8881 gcgatgagca gcaacacatt tactctcggt acaaaatccg ttaaccgtct tggttatggc
     8941 gcgatgcaac tggctggtcc tggggttttt ggccccccaa gagatcgcca cgtcgctata
     9001 accgtgctgc gcgaggcgct ggcattgggc gtcaatcata ttgataccag cgacttttat
     9061 ggtccgcacg tcaccaatca gattatccgc gaagcgcttt atccttactc tgacgacctg
     9121 acaattgtca ctaaaattgg tgcgcggcgt ggagaggacg catcctggtt acccgcattt
     9181 tctccggcag agctgcaaaa agcggtacac gataatctac gtaatctcgg cctggacgta
     9241 ctggatgtgg ttaacctgcg cgttatgatg ggggatggtc atggcccagc ggaaggatcg
     9301 attgaggcca gcctgacagt gctggcggag atgcaacaac aaggtctggt aaaacatctt
     9361 ggcctgagta acgtcacacc gacgcaggtt gcagaggcgc gaaagattgc cgaaatcgtc
     9421 tgcgtgcaga acgaatacaa cattgcccac cgtgctgatg acgcaatgat tgatgctttg
     9481 gctcgtgatg gcattgccta cgtgccgttc ttcccgctcg ggggctttac gccgctgcaa
     9541 tcctccacgc tttccgatgt tgctacaagc cttaatgcga caccaatgca ggtggcgctg
     9601 gcgtggctgt tacagcgttc accgaatatt ttgctgattc ctggcacctc ttcggtgggg
     9661 catctgcggg agaatatggc gtccgatgaa ttagtgctaa cggatgacgt gctggcaacg
     9721 ctggacagta ttgcgcaaaa ggcgggttaa ataaaaaacc tcccactttc gtgggaggca
     9781 tcataaatct tatgcagcaa caatactgtc gatggctgct tttgcgtcag actgtgcttt
     9841 cgctgccatt tccgggccgt acgcgatccc ttcggcgaag acaaatttca catcggtaat
     9901 gccgataaag cccaggaatg tggacagata tggcgttacc agatccgttg ggccatcttt
     9961 atggatccca ccgcggctgg tgataacgat ggctttttta ccggtgacca ggccttccgg
    10021 acccttctcg gtatagcgga aagtaacgcc tgcacgtgca accaggtcaa aatagttttt
    10081 caactgggtc gagatattga agttatacat cggtgccgca ataacgataa cgtcgtgggc
    10141 tttcagctcg gtaatcagct catcggaaag cgccagagct tcctgctgac gcggagtcag
    10201 cggcgcatcg ctcggacgca gtgcaccaac cagttcacca tccaggaccg gaatcggatt
    10261 tgcagccagg tcacgcacgg tgatttcatc agcggagtgc ttttcgcgcc attgttcaac
    10321 aaaataatcg gacaactgat tagactgaga gtaccctgcc aggatgctgg atttaagaac
    10381 taataccttg ctcatggtgt ttccttatag atatttgaat gggcaatgcc ccgttgcttg
    10441 ttgacacttt attcacaatc ctgccacaga gatagcgcaa taaatcgaag cctatgttcg
    10501 aatttattga acaacgcata gaaagctgcg atgtggtact ctatatctat catttaaaag
    10561 aaaattaatc aggcagccta ctgcccacta acgttatgac agaacaacaa aaattgacct
    10621 ttactgcctt gcagcagcgg ctggattcgt tgatgctgcg tgacagattg cgtttttctc
    10681 gccgtctgca cggtgtgaag aaggttaaaa atcctgatgc acaacaggcc attttccagg
    10741 agatggcgaa agagattgac catgcggcag ggaaagtcct gctgcgtgaa gcggcacgac
    10801 cggagattac ttacccggac aatttgccgg ttagtcagaa aaagcaggac attctcgaag
    10861 cgattcgtga ccaccaggtg gtgatcgtcg ccggggaaac gggttccggt aaaacgactc
    10921 agctaccgaa aatctgtatg gagctggggc gcgggattaa agggctgatc ggccataccc
    10981 agccgcgtcg gctggcggcg cgaacggtgg cgaaccgtat tgcggaagag ctaaaaacgg
    11041 agccgggcgg ttgcatcggt tacaaagtgc gtttcagcga tcacgtcagt gataacacga
    11101 tggtcaagct gatgaccgac ggtatcctgc tggcggagat ccagcaagac cgcctgctga
    11161 tgcagtacga caccatcatt attgacgaag cgcacgaacg cagcctgaat atcgattttc
    11221 tgctcggcta tttgaaagag ttgctgccgc ggcgtcctga cctaaaaatc attatcactt
    11281 ccgcgactat cgacccggaa cgcttttcgc gccactttaa taatgcgccg attatcgaag
    11341 tctccggtcg gacctatccg gtggaagtgc gctatcgccc gattgttgaa gaagccgatg
    11401 ataccgagcg cgatcagttg caggcgattt ttgacgccgt agacgaactg agccaggaaa
    11461 gccctggcga cattctgatc tttatgagcg gcgagcgaga aattcgcgat accgccgatg
    11521 cgctgaacaa gctgaactta cgccataccg aaatcttgcc gctttatgca cggctttcca
    11581 acagcgaaca gaaccgcgtg ttccagtcgc acagcgggcg acgcattgta ctggcgacca
    11641 acgtcgcgga aacctcgctg accgtaccgg gtattaaata cgttatcgac ccaggtacag
    11701 cgcgtatcag ccgctacagc tatcgcacca aagtgcagcg tttgccgatt gagccaattt
    11761 cgcaggcttc agctaatcag cgtaaaggcc gctgtggtcg tgtgtccgaa gggatctgta
    11821 ttcgtctcta ttccgaagac gatttccttt cgcgcccgga gtttaccgat ccggagattc
    11881 tgcgtaccaa cctggcctcg gttattttgc agatgaccgc actgggcttg ggcgatatcg
    11941 ccgcattccc gtttgtcgaa gcaccggata aacgcaatat ccaggatggc gtgcgtctgc
    12001 tcgaagaact gggcgcgatc accactgatg aacagcaaag tgcttataaa ctgacgccgc
    12061 tcggtcgtca gctctcgcag ttgcctgtcg acccacgtct ggcgcgtatg gtgctggaag
    12121 cgcaaaaaca tggctgcgtg cgtgaggcga tgattatcac gtccgcgctc tccattcagg
    12181 atccgcgcga gcgtccgatg gataaacagc aggcagctga cgaaaaacat cgtcgcttcc
    12241 acgacaaaga gtctgacttc ctggcatttg tgaatctgtg gaattatctt ggcgagcagc
    12301 aaaaggcgct ttcttcaaac gccttccgtc gcctgtgtcg taccgattat ctcaactatc
    12361 tgcgcgtgcg cgaatggcag gatatctaca cccagttgcg ccaggtggtg aaagaacttg
    12421 gcattccggt taacagcgaa ccggcggatt atcgcgaaat tcacattgcg ttgctgaccg
    12481 gtttgctttc gcatatcggc atgaaagatg ccgataaaca agaatatacc ggcgcacgta
    12541 acgcgcgttt ctccatcttc cccggttctg gtttattcaa aaagccgccg aaatgggtaa
    12601 tggtggcgga actggtagaa accagccgcc tgtgggggcg cattgctgcg cggatcgacc
    12661 cggaatgggt ggagccagtt gctcagcatt tgattaaacg cacctacagc gaaccgcact
    12721 gggaacgggc gcagggcgcg gtgatggcaa cggaaaaagt cactgtttat ggtttgccga
    12781 ttgttgccgc gcgcaaggtc aactacagcc agatcgatcc ggcgttatgt cgtgaactct
    12841 ttattcgcca cgcgctggtg gaaggtgact ggcagacgcg tcacgcattc ttccgcgaaa
    12901 acctgaaact acgggcggaa gtagaagagc tggaacacaa aactcgtcgc cgcgatattc
    12961 tggtggatga cgaaacgttg tttgagttct acgaccagcg catcagtcac gatgtgatct
    13021 ctgcacgcca tttcgacagt tggtggaaaa aagtcagccg cgaaacgcct gatttgctca
    13081 actttgaaaa gagcatgttg atcaaagaag gggcggaaaa aatcagcaag ctggattacc
    13141 cgaacttctg gcatcagggc aatctcaagc tgcgtttgag ttatcagttt gagccggggg
    13201 cagatgctga cggcgtgacc gtacatattc cgctgccgct gcttaaccag gttgaggaga
    13261 gcgggtttga gtggcagatc cccggcctgc gccgtgagct gatcatcgcc ctgattaaat
    13321 cgttgccgaa gccggtacgc cgtaattttg tacctgcgcc aaactatgcc gaagcgtttt
    13381 taggccgcgt cacaccgctg gaattgccgt tgctcgacag ccttgaacga gagcttcggc
    13441 ggatgactgg cgtcaccgtt gaccgcgaag actggcactg ggatcaggtg cccgatcacc
    13501 tgaaaatcac cttccgcgtg gtggatgaca aaaacaagaa gctaaaagaa gggcgctcat
    13561 tacaggatct gaaagacgcc ctgaaaggca aagtgcagga aacgctgtct gccgtggcgg
    13621 atgacggtat cgagcagagc ggtttacata tctggagttt tggtcaactg ccggaaagct
    13681 acgagcagaa gcgcggcaac tacaaagtga aggcttggcc ggcgctggtg gatgagcgtg
    13741 acagcgtagc gatcaaactg tttgataacc cgctggaaca gaagcaggca atgtggaacg
    13801 gtcttcgccg tttactgctg ctaaatattc catcgccgat caagtacttg cacgaaaagc
    13861 tgccaaacaa agccaagctg gggctgtact ttaacccgta tggcaaagtg ctggagctga
    13921 tcgatgactg tatctcctgc ggtgtggatc aactgatcga cgccaatggt ggcccggtct
    13981 ggacggaaga aggctttgct gcgctgcatg aaaaagtccg tgctgaactg aacgacacgg
    14041 tggtggatat tgcgaagcag gtcgagcaaa tcctcactgc ggtgttcaat atcaacaaac
    14101 gtctgaaagg gcgggtggat atgaccatgg cgctggggct ttctgacatt aaagcgcaga
    14161 tgggcgggct ggtgtatcgc ggttttgtca ctggtaacgg cttcaaacga ctgggcgaca
    14221 cgctgcgata tttgcaggca attgaaaaac gactggaaaa actggcggtc gatccacatc
    14281 gcgaccgtgc tcagatgctg aaagtcgaaa acgtccagca ggcgtggcag caatggatca
    14341 acaaactacc gcctgcacgt cgtgaggatg aagacgtgaa agaggtccgc tggatgatag
    14401 aagagctgcg cgttagttac ttcgctcaac aacttggcac gccttatccg atttcggata
    14461 agcgtatttt gcaggcgatg gagcagatta gcggttaaca ctgctatttg cctgataaat
    14521 ctaaaacccg gtaagcattt agcgccgggt tttttattaa ttctaaaaac gataagggaa
    14581 aaaattcagg agttcagaaa aatacaattc tctgctgcaa gatgaataat gtttatctac
    14641 agcatttcct taaaagatat gtcaggcttg cggagtggca gttcaggaaa tacgacttcc
    14701 tcctttcaga gtgctccgct tctcactatt atctcacgca gtattcttaa gggaacgata
    14761 aggaggaacc atgaatatta ccccgtttcc gacgctttcg ccggcaacca tagatgccat
    14821 aaatgttatc ggacagtggc tggcgcagga tgatttctcc ggtgaggtgc cgtatcaggc
    14881 cgattgcgtg atccttgcag gcaatgcggt tatgccgact atcgatgcgg catgtaagat
    14941 tgcccgcgat cagcaaattc ctttactgat tagtggtggt atcggtcact cgacaacttt
    15001 tttgtatagc gccatcgcac agcatccgca ctacaacact atccgcacca ctggcagagc
    15061 agaagctacc attctggcgg atatcgctca tcagttctgg catattccgc atgaaaaaat
    15121 ctggattgaa gaccagtcaa caaactgcgg tgaaaacgca cgctttagca tcgcgttatt
    15181 gaatcaggct gtagaacgag ttcatacggc tatcgttgtt caggacccca ccatgcagcg
    15241 gcgcacgatg gcgacgttcc gccgtataac cggggataat cccgatgcac cacgctggtt
    15301 aagttatccc ggattcgttc ctcagttagg aaataacgca gagagtgtaa tctttgttaa
    15361 tccgttgcaa ggattatggc cggttgagcg ttatctatcg ctactcactg gcgagctgcc
    15421 gcgtttacgc gatgatagcg atggctacgg cccccgcggg cgagatttta tcgttcacgt
    15481 tgattttccg gcagaagtca ttcaggcatg gcaaacgctg aaacatgatg cggtgctcat
    15541 cgaggcgatg gaaagtcgct cgttacgtta aaaattgccc gtttgtgaac cacttgtttg
    15601 caaacgggca tgatttctga cttttatttc tgccttttat ttctgccttt aattcctttt
    15661 acacttgttt ttatgaagcc cttcacagaa ttgtcctttc acgatgccgt ctctctgatg
    15721 attgatgtta attaacaata aatttaccaa aaacaaacat gcaaatcaca ggagtcgccc
    15781 atgtcagtac ccgttcaaca tcctatgtat atcgatggac agtttgttac ctggcgtgga
    15841 gacgcatgga ttgatgtggt taaccctgct acagaggctg tcatttcccg cattcccgat
    15901 ggtcaggccg aggatgcccg taaggcaatc gatgcagcag aacgtgcaca accagaatgg
    15961 gaagcgttgc ctgctattga acgcgccagt tggttgcgca aaatctccgc cgggatccgg
    16021 gaacgtgcca gtgaaatcag tgcgctgatt gtcgaagaag ggggcaagat ccagcagttg
    16081 gctgaagtcg aagtggcttt tactgcggac tatatcgatt atatggcgga gtgggcacgg
    16141 cgttacgagg gcgagattat tcaaagcgat cgtccaggag aaaatatcct tctgtttaaa
    16201 cgtgcgcttg gcgtgactac cggcattctg ccgtggaact ttccgttctt cctcattgcc
    16261 cgcaaaatgg ctcccgctct tttgaccggt aataccatcg tcattaaacc cagtgaattt
    16321 acgccaaaca atgcgattgc attcgccaaa atcgttgatg aaataggcct tccgcgcggc
    16381 gtgtttaacc ttgtactcgg gcgtggtgaa aacgttgggc aagaactggc gggtaaccca
    16441 aaggttgcaa tggtcagtat gacaggcagc gtctctgcag gtgaaaagat tatggcgact
    16501 gcggcgaaaa acatcaccaa agtgtgcctg gaattggggg gtaaagcacc ggctatcgta
    16561 atggacgatg ccgatattga actggcagtc aaagccatcg ttgattcacg cgtcattaat
    16621 agtgggcaag tgtgtaactg tgcagaacgt gtttatgtac agaaaagcat ttatgatcag
    16681 ttcgtcaatc ggctgggtga agcgatgcag gcggttcaat ttggtaaccc tgctgaacgc
    16741 aacgacattg cgatgggacc gttgattaac gccgcggcgc tggaaagggt tgagcaaaaa
    16801 gtggcgcgcg cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg
    16861 aaaggatatt attatccgcc gacattgctg ctggatgttc gccaggaaat gtcgattatg
    16921 catgaggaaa cctttggtcc ggttctgccg gtggtcgcat ttgacacgct ggaagaggct
    16981 atctcaatgg ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg
    17041 aacgtcgcga tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa
    17101 aacttcgaag ctatgcaagg cttccacgct ggatggcgta aatccggtat tggcggcgca
    17161 gatggtaaac atggcttgca tgaatatctg cagacccagg tggtttattt acagtcgtag
    17221 tgagtgaaag aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt
    17281 tcgagggtgc gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt
    17341 ttcaccagtt gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa
    17401 ccgaaatggc tgccaatgat atcggaagag actatttctt catcggtata accaaatgac
    17461 tcgttattgt ttgtcgcctt tttaagcgcg ttgttcacct cttcggcagt cacttttttt
    17521 ccgagaatcg acaccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
    17581 cctttcagtt tgccgctcag ttccgggatc accaggccaa tggcttttgc cgcccccgta
    17641 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgcggg
    17701 ccatccacca gtgactgggt gccggtatag gcatgaatgg tcgtcatcgt gccgacttct
    17761 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt ggtgcatgac
    17821 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattgacgtt ataaacgatg
    17881 gttttcattt caccggcagg ggcggaaatc aacaccttct tcgcgccagc atcaagatgc
    17941 gcctgcgatt tctcggcgga ggtataaaag cctgtacatt cgacaatgat ttctgcgcct
    18001 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
    18061 tcaacgataa gtgaatcttc cgtataatca acgctccagg ggaacggtcc gtagtttgaa
    18121 tcatgtttca gcaggtaggc gagaattttt ggggaagtga gatcattaat agcgacaacg
    18181 tctatgttgc ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa
    18241 ccattaatac caactttact catggtcttc tcctgtcagg aacgttcgga tgaaaattga
    18301 tcctttccag gcttagacca ggatggcggg atgggcaatc tacattcttg cagtgaaacg
    18361 taacgtactg aaaacgggtg aacaatattt aatgaaattt taagaaaagc ccgttatgtt
    18421 aacggcaaat tatgttaaag caggaaatgt tatgggaaat aaatactcaa ggttacaaat
    18481 cagcattcac tggctggtct ttttgctggt tatcgtagcg tattgcgcaa tggagtttcg
    18541 tggtttcttc ccacgtagcg atcggcccct catcaacatg attcatgttt cctgtggcat
    18601 ctcaattctc gtactgatgg tggttcgtct tttgttaagg ctgaaatacc caaccccgcc
    18661 gattatacct aagccaaaac cgatgatgac gggactggcg catttgggcc atttggtgat
    18721 ttatctcctg tttattgcgc tgccggtgat tggtttggtg atgatgtata accggggcaa
    18781 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgagcgggt
    18841 tgatagctta aagtcgtggc atgaaacgct ggcgaatctg ggatattttg tcatcgggtt
    18901 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
    18961 gatgccacgt aaacgttcct gaaggatatt taaagaaaac gcctgtacta aaaccgaccc
    19021 gtggtacagg cgaagaatac gggcctacat cggaagcgcc tattatattt atttgtatga
    19081 caaataaaac cataatcctt gtctatacgt ccatctggct tatctttaat caattcaccc
    19141 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat
    19201 aagattcgat aaatcatgga ggttatatga aaaaactggc acttatcctg gttatgggaa
    19261 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
    19321 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga
    19381 aaaaaacatg cactaactga agtgtaaaag gggggccatg agaaaatgaa tccgctagtc
    19441 agatgcgcgg ggaaaacccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
    19501 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
    19561 tgaatgtcag aatcgtaatg cagataatga gcagacacac caccagaggg ttgtgcttca
    19621 tagcctttct ccttgccgga tggcgggtaa gaggctaaga tctgaattgc taggttcatt
    19681 cgttggcctc ggttgataga aatatcggtc ggggccttcg tctttctgat tccaggttag
    19741 cctgaaaaca gaaagtctca ggcacccgtg agcatcctat gtggtttcct tagggacgaa
    19801 aataatcacc tgacgaaatc gcgtgctgct ttccagaatt tttcgtcatt cggtttagcc
    19861 agtttaatca ttcgttactc tcgcaaggcc gtggatttgc cgttgtcgcc agcgaagtac
    19921 ggacactcgc aagccgcagc gcccaggcgg cgaaagagat tgaaggcttg atcagtgaat
    19981 cagtcaggtt aattgacctg ggatcggatg aggtggcaac ggcagggaaa accatgtgca
    20041 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc accgcctcgg
    20101 gtgaacaaag tcgaggcata acgcaggtta gccaggcgat ttctgaaatg gataaggtga
    20161 cgcaacagaa tgcttctctg gtagaagagg cctcaacggc ggcggtgtcc cttgaagaac
    20221 aggcggcacg actaactgag gcggtggacg tattccgtct gaacaaacat tctgtgtcgg
    20281 cagaaccacg aggagcgggt gaaccagtta gttttgctac ggtgtgaaaa tgttaaagga
    20341 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
    20401 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
    20461 tgcaccatgt cccggaacag gcagggctac cagcgtggca tgacgcaggt cgtcttttac
    20521 agcgccagaa gggacaaacc acacgtaatc gtattcaacc gtaagttgac gagatagcga
    20581 agcagacagc gtttcgatac aacccgaagg aattttacat ccctggctct gcactaatgc
    20641 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
    20701 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcag
    20761 cgattcaaga aacagtagtt cgtaattaag cccgttcatc agttcaggat ctgacatccg
    20821 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
    20881 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
    20941 taatatccct agtgccgcag taggtagcgc accaaccctg acgacatcat tattaagacc
    21001 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
    21061 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
    21121 gcgagcgccc gtcagctgct ccagttcatt caatgtctta gagagcgcag gttgactcaa
    21181 attaagggtt tcagccgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
    21241 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
    21301 aaacgaagcg gtgtcgctga caagtgaagt tgttttatta tgataacttg attgcaaaat
    21361 attattaaca attaaagcaa ctatgttaca gcaaaatgga taatattgat gttttcgcgg
    21421 cgagatcaca gtttgtaaat tcttccagcc ggagtgaatg cggttaccta cactccagat
    21481 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
    21541 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcagta cggcacgctg
    21601 ctggaactgg tagctgatgt aaatctggcg gtgctggaaa acaatcctca actgcacgaa
    21661 aaaatggtaa atgcagacga actggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
    21721 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
    21781 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
    21841 cccattgatg ctgcttctct ggcgcgtaat cccttccgcg tttttacctc attactccgc
    21901 cttgagctta tcgagaacga attattacgc cagaaagcgg cggagattct gcgtcagcgc
    21961 gatatcttca ccccacgttg tcgacaactg ttacaggaat atgagcagcg gggcggtttt
    22021 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
    22081 tcagcaacgg tagatgaaaa aacctatcgc gccttgcaca aagaacatcg gttgattgct
    22141 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgcac gctggatatt
    22201 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct gatcgaaggg
    22261 ccgccgcgcc gcgaggtgcc gattttacta cgccagacca gctttaaagc actggaagag
    22321 acggtgttgt ttgcggggca gaaacagggc acacataccg cgcgctttgg tgaaattgag
    22381 cagcgtggtg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcggaac
    22441 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
    22501 tttcctgaca gtgagttttt aatgcgtcag caagggctgg gatggttccg gtaccgtctg
    22561 acgccttcgg gtgaggcgca tcgtcaggcg attcatcccg gagacgatcc acagccctta
    22621 atagaacgtg gttgggtagt ggcgcaaccc attacctatg aagatttctt gcccgttagc
    22681 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc aggcatgcaa tcacggtgat
    22741 gccagtcgcg aagcatttga gcaggcgttg gggtgtccgg ttttggatga gttccagctt
    22801 tatcaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
    22861 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
    22921 aaaaatgcca atttatgcat attctctgat t
//
LOCUS       DAPQZD010000069        22291 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPQZD010000069 DAPQZD010000000
VERSION     DAPQZD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.069"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            90..305
                     /gene="bdm"
                     /locus_tag="RO454_002592"
     CDS             90..305
                     /gene="bdm"
                     /locus_tag="RO454_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDY0934408.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            407..544
                     /gene="sra"
                     /locus_tag="RO454_002593"
     CDS             407..544
                     /gene="sra"
                     /locus_tag="RO454_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDY0934409.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            700..2397
                     /gene="maeA"
                     /locus_tag="RO454_002594"
     CDS             700..2397
                     /gene="maeA"
                     /locus_tag="RO454_002594"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDY0934410.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2531..3541
                     /gene="adhP"
                     /locus_tag="RO454_002595"
     CDS             2531..3541
                     /gene="adhP"
                     /locus_tag="RO454_002595"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDY0934411.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3727..4004
                     /locus_tag="RO454_002596"
                     /pseudo
     CDS             3727..4004
                     /locus_tag="RO454_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4004..4288
                     /locus_tag="RO454_002597"
     CDS             4004..4288
                     /locus_tag="RO454_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDY0934412.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4338..4991)
                     /gene="fdnI"
                     /locus_tag="RO454_002598"
     CDS             complement(4338..4991)
                     /gene="fdnI"
                     /locus_tag="RO454_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDY0934413.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4984..5868)
                     /gene="fdnH"
                     /locus_tag="RO454_002599"
     CDS             complement(4984..5868)
                     /gene="fdnH"
                     /locus_tag="RO454_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDY0934414.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            complement(5881..8928)
                     /gene="fdnG"
                     /locus_tag="RO454_002600"
     CDS             complement(5881..8928)
                     /gene="fdnG"
                     /locus_tag="RO454_002600"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8341..8343),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDY0934415.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9160..10041
                     /gene="yddG"
                     /locus_tag="RO454_002601"
     CDS             9160..10041
                     /gene="yddG"
                     /locus_tag="RO454_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDY0934416.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10314..11702
                     /gene="narU"
                     /locus_tag="RO454_002602"
     CDS             10314..11702
                     /gene="narU"
                     /locus_tag="RO454_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDY0934417.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            11797..15537
                     /gene="narZ"
                     /locus_tag="RO454_002603"
     CDS             11797..15537
                     /gene="narZ"
                     /locus_tag="RO454_002603"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDY0934418.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            15534..17078
                     /gene="narH"
                     /locus_tag="RO454_002604"
     CDS             15534..17078
                     /gene="narH"
                     /locus_tag="RO454_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDY0934419.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            17078..17773
                     /gene="narW"
                     /locus_tag="RO454_002605"
     CDS             17078..17773
                     /gene="narW"
                     /locus_tag="RO454_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDY0934420.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            17770..18450
                     /gene="narI"
                     /locus_tag="RO454_002606"
     CDS             17770..18450
                     /gene="narI"
                     /locus_tag="RO454_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDY0934421.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            18530..19423
                     /locus_tag="RO454_002607"
     CDS             18530..19423
                     /locus_tag="RO454_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDY0934422.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(19519..20364)
                     /gene="nhoA"
                     /locus_tag="RO454_002608"
     CDS             complement(19519..20364)
                     /gene="nhoA"
                     /locus_tag="RO454_002608"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDY0934423.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            20539..21108
                     /locus_tag="RO454_002609"
     CDS             20539..21108
                     /locus_tag="RO454_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDY0934424.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            complement(21179..22288)
                     /locus_tag="RO454_002610"
     CDS             complement(21179..22288)
                     /locus_tag="RO454_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDY0934425.1"
                     /translation="MSIIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDFGET
                     HLDFLKQYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITPIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         5296 a         5816 c         5691 g         5488 t
ORIGIN      
        1 gaagcagcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc gtagcagaca
       61 ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca gaaaattcaa
      121 cagcagaacc cgctctggtt aatgccattg agcaaggcct tcgggcagag cacggtgttg
      181 tcactgaaga tgacattctc atggagctga ccaaatgggt ggaagcttct gataacgaca
      241 tcctcagtga tatctaccag caaacgatca attatgtggt cagtggtcag caccctacgc
      301 tttaaggtgc tatgcttgat cggcaaccta atttaggggt ttagcacgtg tttcttcgct
      361 acggcgatgt tgtccttaaa actagcaaca ggattgagga gttaaaatga aatcgaaccg
      421 tcaggcacgt catattcttg gactggacca taaaatttct aaccagcgca aaatagttac
      481 cgaaggtgac aaatccagcg tggtaaataa tcccaccggc agaaaacgcc ccgctgaaaa
      541 gtaattcata accatcagtc ctcaatgacg attaaacacc attgcctgcg caatggtgtt
      601 tttgttttta tctgctttat acttggtgcc gacgccctgg cggtaatgca aagacgataa
      661 aagcccccag ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa
      721 cagcgttcgc tttatatccc ttacgctggc cctgtattgc tggaatttcc gttgttgaat
      781 aaaggcagcg ccttcagcat ggaagaacgc cgtaacttca acctgctggg gttactgccg
      841 gaagtggtcg aaaccatcga agaacaagcg gaacgagcat ggatccagta tcagggattc
      901 aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc aggacactaa cgaaaccctc
      961 ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc cagtcattta caccccaacc
     1021 gtcggcgcag cctgtgagcg tttttctgag atctaccgcc gttcacgcgg tgtgtttatc
     1081 tcttaccaga accgccacaa tatggacgat attctgcaaa acgtaccaaa ccataatatc
     1141 aaagtgattg tggtgactga cggcgaacgt attctggggc ttggtgacca gggcatcggc
     1201 gggatgggca ttccgatcgg taaactgtcg ctctataccg cctgtggcgg catcagtccg
     1261 gcctataccc ttccggtggt gctggatgtc ggcaccaaca accaacagct gcttaacgat
     1321 ccgctgtata tgggctggcg taatccgcgt atcactgacg atgaatacta tgaattcgtt
     1381 gatgaattta tccaggctgt gaaacaacgc tggccggacg tactgttgca gtttgaagac
     1441 ttcgcacaaa aaaatgcgat gccgttactt aaccgctatc gcaatgaaat ttgttctttt
     1501 aacgatgaca ttcagggcac cgcggcggta acagtcggca cactgatcgc agccagccgc
     1561 gcggcaggcg gtcagttaag cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga
     1621 tgcggcattg ccgaaatgat catcgcccag acccagcgcg aaggattaag cgaggaagcg
     1681 gcgcggcaga aagtctttat ggtcgatcgc tttggcctgc tgaccgacaa gatgccgaac
     1741 ctgctgcctt tccagaccaa actggtgcag aagcgcgaaa atctcagtga ctgggatacc
     1801 gacagcgatg tgttgtcact gctggatgtg gtgcgtaatg taaaaccaga tattctgatt
     1861 ggcgtctcag gacagaccgg gctgtttacg gaagagatca tccgtgagat gcataaacac
     1921 tgtccgcgtc cgatcgtgat gccgctgtct aacccgactt cacgcgtgga agccacaccg
     1981 caggacatta tcgcctggac tgaaggtaac gcgctggtcg ccactggcag tccgtttaat
     2041 ccagtggtat ggaaagataa aatctaccct atcgcccagt gtaacaacgc ctttattttc
     2101 ccgggcatcg ggctgggtgt tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg
     2161 atgtcggcaa gtgaaacact tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg
     2221 gtactaccgg aactgaaaga tattcagaaa gtctcccgcg caattgcctt tgcggttggc
     2281 aaaatggcgc agcagcaagg cgtggcggtg aaaacgtctg ccgaagctct gcaacaggcc
     2341 attgatgata atttctggca cgccgaatac cgcgactacc gccgtacctc catctaagcc
     2401 tgcgcccggt agtgagggct accgggcaat tttcctctcc ctttttcaga tctcatccat
     2461 actgggtagt ggcgaataaa tctcatttgc ctcacctgct atgcagaaca tcatccgaaa
     2521 aggaggaact atgaaggctg cagttgttac gaaggatcat catgttgacg ttacggataa
     2581 aacactgcgc tcactgaaac atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg
     2641 tcataccgat cttcatgtta agaatggcga ttttggtgac aaaaccggcg tgattctggg
     2701 acacgaaggt attggtgtgg tggcagaagt tggccctggt gttacttcat taaaaccagg
     2761 cgatcgtgcc agcgtggcgt ggttctatga aggatgcggt cattgcgaat actgtaacag
     2821 tggtaacgaa acgctctgcc gttcagttaa aaatgccgga tacagcgttg atggcgggat
     2881 ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa gtgccagatg gtctggactc
     2941 ggcggcggcc agcagcatta cctgtgcggg ggtcactacc tacaaagccg ttaagctgtc
     3001 aaaaattcgt ccaggacagt ggattgctat ctacggtctt ggcggtctgg gtaacctcgc
     3061 cctgcaatac gcgaagaatg tctttaacgc caaagtgatc gccattgatg tcaatgatga
     3121 acagttaaaa ctggcaacca aaatgggtgc agatttagcg attaactcac gcaccgaaga
     3181 cgccgccaaa attgtgcagg agaaagccgg tggcgctcac gcagcggtgg tgacagcggt
     3241 agctaaagct gcgtttaact cggcggtgga tgctgtccgt gcaggtggtc gtgtcgtggc
     3301 tgtcggtctg ccgccggagt ctatgagcct ggacatccca cggctggtgc tggatggtat
     3361 tgaagtggtc ggttcgctgg tcggcacgcg tcaggatcta accgaagcct tccagtttgc
     3421 cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg ttagaggaca tcaacaccat
     3481 ctttaccgag atggaagaag gcaaaatccg tggccgtatg gtgattgatt tccgtcgcta
     3541 agaggtcttt gctgcgactg ccatgttcgg gtcgcagcct gtttcgcgag acgctttccg
     3601 taatttcact cttcttcttt tcctccccca tatttcccct cacaattcgc gaaaacgtag
     3661 cgaaatttgt tgcgctgcac ggaacaatag tgatagtgtt ccttttgagg gaacaaggat
     3721 gaagagatga taatgaattt cagacacaag ggattgcgtg acttgtttct tctcggcaaa
     3781 acctccggcg ttataccgac gcaagtcaaa cgattacgcc accactcgct gtgattgatg
     3841 cagcatgctg tcttgctgat atcgatatgc ccggttaccg actacatccg ttaagcggcg
     3901 atcgcgatgg aatttgggcg atatctgtct cgggcaactg gcgaatcaca tttgaattcg
     3961 tcaatggcga tgcatacata ctggattacg aggactatca ctgatgaaaa tggccaatca
     4021 tccccgcccg ggggacatta ttcaggaatc actggacgaa cttaatgtca gcctgcgcga
     4081 gtttgccaga gcaatggaaa ttgcgccctc aacggcaagc cgattgctga ccggaaaagc
     4141 agctttgacg ccagaaatgg caataaaact ttccgtggtg atcggcagtt cgccgcaaat
     4201 gtggctgaat ctgcaaaatg cctggagtct ggcagaggcg gaaaaaacgg tggatgtgtc
     4261 gcgactgcgc cgtctggtta cgcaataaaa aatggcgctt cttaaagcgc cattttcagc
     4321 ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc tgccttctcg
     4381 atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac cttcccttcg
     4441 atcatccctt taatcgatcc tttcacccag aatgccatat acatatggat caggatggcg
     4501 tggatcagga tgatacccgc agccgcatgg atcagcaggc tataacggac aacctgtatc
     4561 gggaagtact gcgcaaagta cggacgccag ataatcactc cggtcaccag cagcacgaaa
     4621 atcatgctca tgattgacca gaacatcatc ttttgcccgg cgttgtactt accgacatcc
     4681 gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca cggaatatct
     4741 ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag cgcgacgaaa
     4801 atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc
     4861 gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac caggaagaag
     4921 caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac aatcattttc
     4981 gacttactca tgatttccct cctcctcatc gtccacttcc ttattcgggc caataccgat
     5041 gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca gcggtttcaa
     5101 cgcgcctttc cacaggctta ccgaggtatc gatcttcgga tctttcggca gaccatgata
     5161 cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac cgaccccttc
     5221 cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca cgcgctgttc
     5281 cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg gacaggtttt
     5341 cacacaagcc ggttcctggc cgacgctgac gcgatcgaca cagagcgtgc atttatatac
     5401 ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa tgcagtaacc
     5461 acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact gaatgattgc
     5521 accagcagac gggcatgcct tcaggcagcc cggatcttca cagtgcatac agccgtcttt
     5581 acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca
     5641 ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc ccacttcatc
     5701 acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga tacaagtgga
     5761 aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag gcggcgtgat
     5821 ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc atagccatct gttcgccccc
     5881 ttacgccttc tcgatgttta ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc
     5941 accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac cttcaaagcc
     6001 ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca gcggtttcag
     6061 acggcgcgtt accacagcca ccgcgcggat aaagccacgc ttgctggaga cagtgacacg
     6121 atcgccattg gcaatgcctt tcgccgccgc cagcgtttcg ctgatttcca caaactgttc
     6181 cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt gctccgtcag
     6241 acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca gcgcgtcttg
     6301 ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcaacgggt tagtgcccag
     6361 cggcgtttca attggctcgt agtgttccgg gaacggacct tccgccattt tgttgatggc
     6421 aaacaggcgt cccatccctt ccggttgcat gataaacggc ccggttggcg tacccggcgc
     6481 ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctaccgttcc actggatcag
     6541 catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt tgtagagtac
     6601 gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac cggacgggtc
     6661 ggagttatcg cggttagcca tctggttgcc ctgctcggtc cagctaccgg tgtagatcca
     6721 gcaagaagat gcggttgtac cgtcatcacg cagatgtgca aagctactca gcaactgacc
     6781 tttcttcgca atcagcacgc cattagcgtc atagagatct tccagcgcat agccgttatt
     6841 ctctttcgcc acttcatcag actgcggttc gtgcggctgc ttgtagttcc agctcatctt
     6901 catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca gatgatggta
     6961 gatacccgcc agaatttcgc cgtcgttacg tgcttcgccc ggcgcgtcct gacctttcca
     7021 gtgccactgc aaccagcgac cggagttagc gatagaacca tcttcttcag caaagcaggt
     7081 cgaaggcaga cggaatactt cagtctgaat agatgccgga tcgacatcgt tcgactcgcc
     7141 gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca ccatgtactt
     7201 cagcttgctc aggcagctca ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa
     7261 gccctggcag aaataaccgg tgactttacc ttcatccatc atgttgaaat acttgatgac
     7321 gtcgtaggtc tggtcccact tcggcagcca gtcgtagccc cagttgttct ctttctgcgc
     7381 ggcatcgccg tagaaagatt tcatcaggct aacgaaaaac ttcggatagt tgctccagta
     7441 gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg actgcaaatc
     7501 aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga gcaggcctaa
     7561 gtcagtcagg ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc caccggccat
     7621 acccatgtta ccgagcagca actggatcat cgccatcgta cggatgttct gcgcacccac
     7681 ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt
     7741 ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc aaatgttttc
     7801 tactacgtcc ggcgtgtagc gggaaacgtg cgctttcagc agattccata cacagcgcgg
     7861 atgagtcagc gtgtcatcgc gtttcgcata gccgttttca tcgaactgat agttccagga
     7921 cgacttatcg tattgacgtt tttcagcgtc gtagccgctg aacagaccgt cttcgaaagc
     7981 aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgc ttaacgtatt cggcgttgat
     8041 tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga
     8101 acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg gatcgacaac
     8161 gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg
     8221 cgcttcagca gcgttaccgc ccatcaccat tacgacgtta gcgtttttga tatccaccca
     8281 gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg
     8341 tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa atttttgagt
     8401 cagcatcccg gtttcgttgc tggcaccgga agcacacagc ataccggtag aaagccaacg
     8461 gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt cggctttcat
     8521 cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt tgtcagaacc
     8581 tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacataat ccagcaaacc
     8641 ggcccctttc gggcacagcg caccacgact taccggatga tccgggtcac cttcaatgtg
     8701 ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca atagcccgca
     8761 acctacggaa cagtatgtgc aggtgttacg gatctctttc gcgcgtaata atttgtagtt
     8821 tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gctgctactg ttgttcccgc
     8881 cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg ctttcctctt
     8941 ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga
     9001 aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg acctacctca
     9061 atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc tataaaattc
     9121 agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgtca aaaagcaacg
     9181 ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc
     9241 agtgagggac tcggcccggt cggcggcgca gctgctatct attcattaag cgggctgctg
     9301 ttaatcttca cggttggatt tccgcgtatt cggcagatcc cgaaaggcta tttactcgcc
     9361 gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg
     9421 acccgtcatc aggcgattga agtggggatg gtgaactatc tatggcccag cctgacaatt
     9481 ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc aggattatta
     9541 ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaca atgggttaca ttatgatgaa
     9601 attatcaata atatcaccac cagcccattg agttatttcc tggcgttcat tggggcgttt
     9661 atctgggcag tctactgcac agtaacgaat aaatacgcac gcggatttaa cggaattacc
     9721 gtttttgtcc tgctaacggg agcaagtctg tgggtttact attttcttac gccacaacca
     9781 gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt taccttagga
     9841 tttgcttatg ctgcatggaa tgtcggtata ttacatggca acgtcaccat aatggcggta
     9901 ggttcgtatt ttacgcccgt cctttcctca gcgcttgcag ccgtgctgct cagcgccccg
     9961 ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct gctctgctgg
    10021 ctggcgacac gtcgtggcta aaataattgt cgggtctacg gcccggcagt taatcagata
    10081 aattctgaca atattaaaaa ataaatttca aataccagaa agtcatttta gttacattta
    10141 gtaacacata tctgtctgtt taatccacat tctgcccccg cagttacgct ttcttatcta
    10201 ttcttctaat cagcacttca cctgattcat ccttaattct aataactggc tattgcgcct
    10261 tacctcctgt tttgtctttt tttcattacc aatgtgtgca tgtgaggaac aatatggcac
    10321 tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg gaaaccagaa aatccggcct
    10381 tctgggaaaa taaaggaaag catattgcac gaagaaacct ctggatatca gtcagttgtc
    10441 tacttcttgc cttctgtgtc tggatgctat ttagcgcagt tactgttaat ctcaataaaa
    10501 tcggttttaa ttttactacc gatcaactct ttttattaac cgcattaccc tccgtttctg
    10561 gcgcattatt gcgtgtcccc tactccttta tggtgcctat attcggtgga cgccgatgga
    10621 cggtttttag tactgcaatc ctgattattc cttgcgtctg gctcggaatt gccgtgcaga
    10681 atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt tttgcaggtg
    10741 cgaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc aaacaaggga
    10801 gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg cagctggttg
    10861 caccgctggt catttttgtc cccgtatttg cctttctcgg cgtcaatggc gtaccgcagg
    10921 ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtgcca ttactggcga
    10981 ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc gcctcaattg
    11041 ccgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg ctttaccttg
    11101 ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc catgctggca aaaacccagt
    11161 tcccggatgt gaatattctg cgcctggcgt tctttggccc atttatcggt gccatcgcgc
    11221 gatcggttgg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg ttgatcaact
    11281 tcatttttat ggcgattttc agcgccctgc tgttccttac cttaccgggc acaggctccg
    11341 gtaatttcat cgcattttac gccgtattta tggggctgtt tctgaccgcg ggtctgggaa
    11401 gtggttctac tttccagatg atcgccgtca tctttcgcca gataacaatt tatcgggtga
    11461 agatgaaagg cggtagtgat gagcaagctc aaagagaagc cgtcaccgaa acggcagccg
    11521 ctctgggctt tatctcagcc attggcgcag tgggcggctt ttttattccg caggcgtttg
    11581 gcatgtcgct caatatgacc ggctctccgg tgggcgcgat gaaagtgttt ttaatcttct
    11641 acatcgtttg tgtgctactg acctggctgg tttatggtcg gcggaagttc agccaaaaat
    11701 aagagatcga tgcctgacac gtcttatcag gctacaaccg tgtcgcatcc gacaaggcat
    11761 cagatcccaa ttcattcctg gagcaggagt tatgtcatga gtaaactttt ggatcgcttt
    11821 cgctacttca aacaaaaggg cgaaatcttt gccgatggtc acggacaggt gatgcatagc
    11881 aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat cgtgcgttcc
    11941 acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa aaatggtctg
    12001 gtgacctggg aaatccaaca gaccgattac ccgcgcactc gccctgacct gccgaatcac
    12061 gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag cgcgaaccgc
    12121 ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgcgaagc cctcaagcaa
    12181 cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa gtgcctgagc
    12241 tacaaacaag tgcgtggacg cggtggtttt atccgctcca actggcagga gctaaatcag
    12301 ctgattgccg ccgctaacgt ctggaccatc aaaacttacg gcccggatcg cgttgccggt
    12361 ttctcgccga ttccggcgat gtcgatggtt tcttacgctg ccggaacacg ctatctgtcg
    12421 ctgttaggtg gcacctgttt aagcttctac gactggtatt gcgacctgcc gcccgcctcg
    12481 ccgatgacct ggggcgagca aaccgacgta ccggaatctg ccgactggta taactccagt
    12541 tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc ccatttcttt
    12601 accgaagtac gctacaaagg cactaaaacc atcgccatta cccctgacta ctctgaagtg
    12661 gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagtgc cctggcaatg
    12721 gcaatgggcc atgtcatttt aaaagagttt catctcgata atcccagcga ttattttatc
    12781 aactactgcc gccgctacag cgacatgccg atgctggtaa tgctggaacc tcgcgacgac
    12841 ggcagctacg ttcccgggcg catggtccgc gcatctgacc tggtggatgg actgggcgaa
    12901 agcaataatc cgcagtggaa aaccgtggca gttaataccg caggtgaatt ggtagtgccg
    12961 aacggttcga ttggttttcg ttggggagaa aaaggcaaat ggaacctgga atccattgcc
    13021 gccggtacgg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc cgttgcaggc
    13081 gtggccttcc cctactttgg aggcattgaa aatccgcatt ttcgcagcgt aaaacacaat
    13141 cctgtcctgg tgcgccaatt gcccgttaaa aatctgacat tagccgatgg tagcacctgt
    13201 ccggtggtca gcgtttatga tctggtactg gcaaattacg gcctcgatcg cgggctggaa
    13261 gatgaaaaca gcgcgaaaga ttacgctgaa atcaaacctt acacccctgc ctggggcgag
    13321 caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgtgaatt tgccgatacc
    13381 gctcataaaa cgcatgggcg ctcgatgatt attctcggcg caggtgttaa ccactggtat
    13441 cacatggaca tgaactaccg cgggatgatc aatatgctga tcttctgcgg ttgtgtcggg
    13501 caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc acaaaccggc
    13561 tggttgccgc tggcctttgc gttcgactgg aaccgtccac cgcgccagat gaacagcacc
    13621 tcgtttttct acaatcactc cagccagtgg cgctatgaaa aagtcaccgc gcaggaattg
    13681 ctctcaccgc tcgccgatgc cagtaaatac agcggtcacc tgattgattt caacgttcgc
    13741 gccgaacgta tgggctggct cccttctgcg ccgcagctgg ggcgtaaccc gctctccctg
    13801 aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca ggcgctgaaa
    13861 tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca tccgcgtaat
    13921 ttgtttgtct ggcgttctaa cctgcttggc tcctccggca aaggccacga gtatatgcag
    13981 aagtatctgc tggggaccga aagcgggatt cagggagagg aactcggtgc cagcgacggg
    14041 atcaaaccag aagaagtcga gtggcaaact gcggcgattg aaggcaagct cgacctgctg
    14101 gtgacgctcg atttccgcat gtccagtacc tgcctgttct ccgatatcgt tctgcccacc
    14161 gccacctggt acgaaaaaga cgatatgaat acctcggata tgcatccgtt tattcatccg
    14221 ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat ctacaaaggt
    14281 atcgctaaag ccttttcgca agtgtgcgtg gggcatctcg gcaaagaaac cgacgtggta
    14341 ttacaaccac tgctgcatga ctccccggca gagctttcac agccgtgtga agtgctcgac
    14401 tggcgcaaag gcgagtgcga tctgattcca ggcaaaaccg cgccgaatat tgtggcggtg
    14461 gagcgcgact accctgctac ctatgaacgc tttacctcgc tcgggccatt gatggacaaa
    14521 cttggcaacg gaggtaaagg gatttcgtgg aatacccagg atgaaattga tttcctcggt
    14581 aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat tgataccgcg
    14641 attgatgcct cagaagtgat tctggcgctg gcaccggaaa ccaacggcca tgttgcagtc
    14701 aaagcgtggc aagcgctggg cgagatcacc ggacgcgaac atacccatct ggcgctgcac
    14761 aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa aattatctcc
    14821 agccccacat ggtctggtct ggaaagcgat cacgtctcct ataatgcggg atacaccaac
    14881 gttcatgagc taattccgtg gcgcacactg tcgggccgtc aacagcttta tcaggatcat
    14941 ccgtggatgc gtgcctttgg tgaaagcctg gtggcttatc gcccgcctat cgacacccgc
    15001 agtgtcagtg aaatgcgcca aatcccgcca aacggcttcc cggaaaaagc ccttaacttc
    15061 ctgacaccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct gctaatgctg
    15121 acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg tgaactgacc
    15181 attgtcgata acgactgggt ggaagtgttt aacgccaatg gcgcgctgac ggcccgcgcg
    15241 gtggtcagcc agcgtgtacc gccgggcatg accatgatgt atcacgctca ggaacgcatt
    15301 atgaatattc ctggttcgga agtaaccggc atgcgcggcg gcattcataa ctcggtcacc
    15361 cgcgtttgtc cgaaaccaac acatatgatt ggcggttacg cgcagctggc ctggggcttt
    15421 aactactacg gcactgtcgg ctctaaccgc gacgaattca tcatgatccg caagatgaag
    15481 aacgttaact ggctggatga tgaaggtcgc gatcaggttc aggaggcgaa aaaatgaaaa
    15541 tacgttcgca agtcggcatg gtgcttaacc tcgataaatg tatcggctgc catacctgtt
    15601 cggtgacctg taaaaacgtc tggaccggtc gcgaaggcat ggagtacgca tggtttaaca
    15661 acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag gatgagtggc
    15721 aaggtggctg ggtgcgtgat gtgaatggca agatacgccc gcgtctgggc agcaagatgg
    15781 gcgtaataac caaaattttc gccaacccgg tagtgccgca gattgacgat tactacgaac
    15841 ctttcacctt cgactacgaa catttgcata gcgcgccgga aggcaaacat attcccaccg
    15901 cccgtccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg ggcccaaact
    15961 gggaagaact gctgggcggc gaatttgaaa aacgagcccg cgaccgcaac ttcgaggcca
    16021 tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt
    16081 gcgaacactg cctcaatccc agctgcgtgg cgacctgccc cagcggcgct atctacaaac
    16141 gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca
    16201 ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat
    16261 gtatcttctg ttatccgcga attgagtccg gtcagccaac agtctgctcc gaaacctgcg
    16321 tgggtcgcat ccggtatctg ggcgtgctgc tctacgacgc cgaccgcatc gaagaagcag
    16381 cgagtaccga acgagaggtc gatctctatg aacgccagtg cgaagtgttc ctcgatccgc
    16441 acgatccctc agtgatcgaa gaagccctga aacaaggtat tccacaaaac gtgattgacg
    16501 ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg ctaccgctgc
    16561 accctgaata ccgcaccctg ccgatggtct ggtacgttcc tccgctgtca ccgattcagt
    16621 cctacgctga tgcgggaggt ttgccgaaaa gcgaaggcgt gctgcccgcc atcgaaagcc
    16681 tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggtgatacc ggtccggtac
    16741 tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa accgtggaag
    16801 gcgttactga tactcgtgcc atcgacgaag taggtctgag cgtcgcccag gtcgaagaga
    16861 tgtatcgtta cctcgccatt gccaactatg aagatcgttt cgtcatcccg acaagccatc
    16921 gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc tttggcgacg
    16981 gttgccacgg ttcggacagc aaattcaacc tgttcaacag tagccgtatc gatgccatca
    17041 acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca aagtgctcgg
    17101 cctgttgatg gagtacccag acgagttgtt gtgggagtgt aaggacgacg cgctggcgct
    17161 tatccgccgc gacgcgccga tgctcaccga ttttgcactc gacctgctca acgccccgct
    17221 gctggataaa caggccgaat ggtgtgaagt gtttgaccgc gggcgcacca cgtcgctgct
    17281 gctgtttgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg tggacctgct
    17341 ggcggagtat gaaaaggtcg gtctgcaact gaattgtagg gaactgcccg attatctacc
    17401 gctgtatctg gagtatttaa gcgtacttcc ggacgatcag gcgaaagaag gattactcaa
    17461 cgttgcgccg atcctcaccc tgcttggcgg acgcttaaaa caacgcgagg caccgtggta
    17521 cgcgttattt gacgccctgt tgcaactggc aggaagccct ctgtcaagtg acagtgtcac
    17581 aaaacagatt cgcagtgaag agcgtgacga cacccgccag gcgctggatg cggtgtggga
    17641 agaagaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct cgccgcttaa
    17701 tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca tcagtgcggg
    17761 aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc gtacttgtgt
    17821 ggcacggttt ttatccttgg aagctggctg cgctacgact acgggcagta cacctggcgc
    17881 gccgcctcca gccagatgct ggataaacgc gggatggtgc tgtggtcaaa cttgttccat
    17941 atcggcattt tggggatttt cttcgggcac ctgttcggca tgttaacgcc acactggatg
    18001 tacgcgtggt ttctgccggt ggcggtaaaa cagcagatgg cgatgatcct cggcggaata
    18061 tgcggggtat tgacgctgat tggcggtgcg gggctactgg tgcgtcgttt aacaaatccg
    18121 cgtatcaggg cgacgtcttc tggtgcggat atcctgatcc tcgccatttt gttaattcag
    18181 tgcattctcg ggttaaccac cattcctttt tcagcacaac atccggacgg cagtgaaatg
    18241 atgaagctgg tgggctgggc gcaaagcatt gtcactttcc agggcggtgc atctacgcat
    18301 cttgatggtg tcgcctttat attccgcgtt catctggtgc ttggaatgac catcttcctg
    18361 ctcttcccgt tcacccgact ggtgcatgtc tggagcgcgc cgtttgagta ctttactcgt
    18421 cggtatcaga ttgtgcgttc gcggcgttaa ttctcacgcg ggccggatag cccttcgctc
    18481 tccggccact tgcaggctat gatagttctc cagccacagg agaacgttaa tgaaaccgca
    18541 agtgtaccac gtcgatgctt ttacctctca accgtttcgc ggcaattctg ccggtgttgt
    18601 tttccccgcc gataatctca gtgaagcgca aatgcagctt atcgcccgcg agttaggtca
    18661 ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc gctactttac
    18721 gccaacggtt gaagtgccaa tttgcggtca cgcgacggtg gctgcgcact atgtacgttc
    18781 aaaggtatta ggtttgggaa attgcacggt ctggcaaacg tcgctggcag gaaaacatcg
    18841 tgtgactatc gaaaagcaaa atgatgatta tcgtatttcg ctggaacaag gtacgccagg
    18901 ctttgagcca ccactgacag gtgaaacacg tgcagcgatt atcaacgcgc ttcatctcac
    18961 tgaggacgat attctgccag gcttgccaat tcaggtggca acaacaggac actcaaaagt
    19021 gatgatcccg ttgaaaccag aagtggatat cgacgccctt tcgcctgatc ttgctgcgtt
    19081 gaccgctatc agcaaacaga ttggctgcaa tggtttcttc ccgttccaga tccgcccagg
    19141 caaaagcgaa accgacggtc gcatgttctc gcctgcgatt ggtattgtgg aagatccggt
    19201 gaccgggaat gccaatggtc cgatgggcgc atggttagta catcacaacg tattgcccca
    19261 cgatggcaac gtgttgcgtg ttaaaggcca tcaggggcgc gcattggggc gtgatggtgt
    19321 aattgatgtg acggtgacaa ttcgcgataa ccaaccggag aaagttacca tttccggcac
    19381 ggcggtgatt ctattccatg ctgaatgggc cattgatttt tgatgccgtt gcctgatgcg
    19441 cttcgcttat caggcctaca accggactaa tgtaggccgg ataaggcatt tacgccacat
    19501 ccggcaacct gacataaatt attttcccgc ctccgggtgc gtatcaaacg ccgccatcac
    19561 cagcgctaac tcatccacag taaagccatg tttcgcatca tctacgccca gaccaaactg
    19621 ttcctgcatc acggcatata acgacgccac atctggtaga tttcgctgct caactgcgtg
    19681 cccattttca taatgggtaa aatgaaaatt ggtcagcgtt agcttgccac cgtccggcaa
    19741 atggcgacac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg ccgaccagaa
    19801 attgcctatc acataatcgc tttgttgctg ctcgcagaga tcaaaacggt acatcgactg
    19861 ccagtgctga tgatgattaa actgcaatac ccaatcatca ccctcttgca acaaccgata
    19921 ctctccgtgt ggcgtggttt gcatgatatc cggcattaaa cgaatcggcg cggttagcgt
    19981 ctgcccaccg aaaccgacat cagcaatcca tttttcccct tccagttcca ccaacagcaa
    20041 acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgac ctaacaagct
    20101 gcgaacgtta aaccccagct cgcgtaacac ccgctcaaac acgccattct gctcaaaaca
    20161 gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat caagctgtat
    20221 ttccctcggc agcaaaacgt cgagattttc aaacggaatg gtgcaattgt gtttcaggtg
    20281 caatgcacgc agcgtatcaa tattggccgc agcagctccc aaccagttaa tacgggcaaa
    20341 ataatgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc aacttataac
    20401 ctggaataga cgccgcactg gctattttgt gcgatcgcgg acatgcatca cgttcgcaaa
    20461 tcgccaatcc cgttaatttc gttacttatt gcgttacgag ctacgctttt aaaacaccct
    20521 tcatagcgag gcgatatcgt gagccaattc attcccgtcg aattacacca tgccagctgt
    20581 ctgttaaatc atggcccaac tgtcatgatc accagtttcg atgagcaatc ccagcggcgt
    20641 aacattatgg cagcagcctg gtcgatgccc gtagagtttg aaccgccacg cgtggcgatt
    20701 gtggtagata aatcgacatg gaccagggag ttgattgaag gtaacggtaa atttggcatc
    20761 gttatcccgg gcgttgcggc gactaactgg acgtgggcgg tgggaagtgt gtcgggtcgt
    20821 gaagaagata agtttaattg ctacggcatt ccggtagtga gaggcccggt gcttggtttg
    20881 ccgctggtcg aggaaaaatg tctggcgtgg atggagtgtc gcctgctacc tgcgacttcc
    20941 gcgcaagaaa aatacgatac gctatttggc gaagtggtat ctgcagcggc agacgcacga
    21001 gtatttgtcg aaggccgctg gcagtttgat gatgataagc tcaatacgtt gcatcattta
    21061 ggtgctggca tgtttgttac cagtggtaag cgagttacgg caggttaagc attacatgct
    21121 gtaaccgggt ttctttatca aagcctccat ttggggcgcg atttcggcca gggcaagatt
    21181 acgaaagccc gctccccgca aggactgacg cgagatagtt tctgtccatg gctgcttttc
    21241 gcatcttacg tcttaaccct gccttgaata ccttatcatt cgttaaaata ttaatagcga
    21301 tgtgccgtat ccctgaaaat aattctgcgg cgtttcctct tcttattttg cagtcgtctt
    21361 cattcattac cacgtccaga cgccagtgca gcttattctc cacgtgccag tggtttcgga
    21421 ttgctgtggc gaacttctct gcggttaaat cagcagaact gatataatat ctgaccgtca
    21481 tttctggctc tttcttttgt tctgctatta tggaccgaaa ggagactgcc acgcataatt
    21541 tcttcagtcc tttccattcg aacgtgaaat caataagttc atcagggaca tcgcaaacaa
    21601 tatgaagacg gatttcttct ctgccgtgac tcttttcact aatggcgtaa ctgtcatgtt
    21661 ctggattatt taattctttc agtggaaatt tttcctcaaa ggctttatta agccgcccct
    21721 gatttccttt tacagcgaat aaataatcac cgccctgttt ttgtatcttc tctgcaatat
    21781 ctttctggca acccatcgca tcagttgtga tgatttttcc tttaatatcc agcatgttaa
    21841 gaagttcagg gataggtgta atttcattgg atttctcatc cgtcctgatc tgtccgatga
    21901 ccagactgtg cattgttgag aacgcactaa tgacatgaat cgctcccttg cggcgactct
    21961 tgtcataaga atgccggagt gtttttccat caattgcgat gacgtcttta tcatctgaag
    22021 aatggcagtc acgcatccag ttaataaagc actcgtgaaa ttttgcagga ctgatacagg
    22081 atacaactct ggcaatggta tcgtgaacag gaataccatt atcaaaatca ccatattgct
    22141 tcaaaaaatc gagatgtgtt tccccaaaat cctctatatc ttcccagcct tctgcacccg
    22201 aaataacggc acaaatagtc aacagtagaa tatccgataa tttatgttcc actttccagg
    22261 cttgtctgta atcggggata atagaaatat g
//
LOCUS       DAPQZD010000070        22222 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPQZD010000070 DAPQZD010000000
VERSION     DAPQZD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.070"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(147..1058)
                     /gene="gsiD"
                     /locus_tag="RO454_002611"
     CDS             complement(147..1058)
                     /gene="gsiD"
                     /locus_tag="RO454_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDY0934426.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1061..1981)
                     /gene="gsiC"
                     /locus_tag="RO454_002612"
     CDS             complement(1061..1981)
                     /gene="gsiC"
                     /locus_tag="RO454_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDY0934427.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGILLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1999..3537)
                     /gene="gsiB"
                     /locus_tag="RO454_002613"
     CDS             complement(1999..3537)
                     /gene="gsiB"
                     /locus_tag="RO454_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDY0934428.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESCVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3557..5428)
                     /gene="gsiA"
                     /locus_tag="RO454_002614"
     CDS             complement(3557..5428)
                     /gene="gsiA"
                     /locus_tag="RO454_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDY0934429.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5415..6380)
                     /gene="iaaA"
                     /locus_tag="RO454_002615"
     CDS             complement(5415..6380)
                     /gene="iaaA"
                     /locus_tag="RO454_002615"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDY0934430.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACLMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6584..7819
                     /gene="moeA"
                     /locus_tag="RO454_002616"
     CDS             6584..7819
                     /gene="moeA"
                     /locus_tag="RO454_002616"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDY0934431.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7819..8568
                     /gene="moeB"
                     /locus_tag="RO454_002617"
     CDS             7819..8568
                     /gene="moeB"
                     /locus_tag="RO454_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDY0934432.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8644..9306)
                     /gene="fsa"
                     /locus_tag="RO454_002618"
     CDS             complement(8644..9306)
                     /gene="fsa"
                     /locus_tag="RO454_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDY0934433.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAVGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9437..10336
                     /locus_tag="RO454_002619"
     CDS             9437..10336
                     /locus_tag="RO454_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDY0934434.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10342..12774
                     /locus_tag="RO454_002620"
     CDS             10342..12774
                     /locus_tag="RO454_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDY0934435.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12920..13735
                     /gene="ybiV"
                     /locus_tag="RO454_002621"
     CDS             12920..13735
                     /gene="ybiV"
                     /locus_tag="RO454_002621"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDY0934436.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHITLDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(13935..15527)
                     /locus_tag="RO454_002622"
     CDS             complement(13935..15527)
                     /locus_tag="RO454_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDY0934437.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            15746..16666
                     /gene="ldtB"
                     /locus_tag="RO454_002623"
     CDS             15746..16666
                     /gene="ldtB"
                     /locus_tag="RO454_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDY0934438.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16724..17842)
                     /locus_tag="RO454_002624"
     CDS             complement(16724..17842)
                     /locus_tag="RO454_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDY0934439.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(17839..18306)
                     /gene="mntR"
                     /locus_tag="RO454_002625"
     CDS             complement(17839..18306)
                     /gene="mntR"
                     /locus_tag="RO454_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDY0934440.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18492..18620
                     /gene="mntS"
                     /locus_tag="RO454_002626"
     CDS             18492..18620
                     /gene="mntS"
                     /locus_tag="RO454_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDY0934441.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18892..20475
                     /gene="opgE"
                     /locus_tag="RO454_002627"
     CDS             18892..20475
                     /gene="opgE"
                     /locus_tag="RO454_002627"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDY0934442.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20524..21039)
                     /gene="ompX"
                     /locus_tag="RO454_002628"
     CDS             complement(20524..21039)
                     /gene="ompX"
                     /locus_tag="RO454_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDY0934443.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21092..21157)
                     /locus_tag="RO454_002629"
     CDS             complement(21092..21157)
                     /locus_tag="RO454_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934444.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            21392..>22222
                     /gene="rhtA"
                     /locus_tag="RO454_002630"
     CDS             21392..>22222
                     /gene="rhtA"
                     /locus_tag="RO454_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDY0934445.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     M"
BASE COUNT         5428 a         6095 c         5488 g         5211 t
ORIGIN      
        1 acgctgtgcc gctgcaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
       61 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
      121 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
      181 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaat
      241 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgcaccccac
      301 tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc
      361 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaagata
      421 tgacgcaaca aaatggtcat atcgctggca ccaatactgc gtgctgactc gataaaggtt
      481 tgctgtttca acaccagcgt gttgccgcgc accagccggg caaacgcggg gatggaaaaa
      541 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
      601 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
      661 caccagcctt cataatatcc agccagcaag cccagcaacg tcccgatcgc cgcaccgata
      721 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
      781 atgtcgcgcc ccagtgaatc gacaccaaac cagtgctgca aagaaggtcc gttattcaga
      841 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
      901 accacaatca ataaaataac gaacaatgcg gcggtcatcg ccatatgctg acggcgaaat
      961 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1021 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1081 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     1141 aaagcagaat ttccgcctga atcactgggt aatcgcgcat ttctacggag tcaacgagta
     1201 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatggaacca ccgagcaaga
     1261 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     1321 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     1381 ttaaaacgtc gacaaacgac gcgcgggtaa agcgcgccat cacggcggcc actgccgcgc
     1441 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc
     1501 agcccagttc aacggagaat acctgaatta aaagtatccc cagagcaaat gccggaaacg
     1561 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacggtta cgccagacgg
     1621 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     1681 gccagagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     1741 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     1801 gcggctgatc caaccccaac tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     1861 gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg gacaaataaa aacaccagca
     1921 ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg tttgataacg taattaagca
     1981 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg
     2041 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     2101 cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttataca agcgggtctt
     2161 ttccgccgga tcattagttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     2221 gtaaaacgcg gtattaaaca gcgttggtgg ccagttctgc gaggcaaaca gcggcgatag
     2281 agcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgcagct
     2341 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcagtcac
     2401 ctgggcttta atcccaacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     2461 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     2521 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     2581 ggcgatactc ggcggtacca caccagtagc tggcgttgca tagcccgcga aggccacttt
     2641 caccagcgcc gggcggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     2701 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     2761 ctcgatattt ttgtttttct ccagcagtgc ggcctgctcg tagggaatgg ggaaagcaaa
     2821 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     2881 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     2941 cacaaaatcg gtctgattcc aggtatccag ttcatacggc ccggttccca ccggatgaaa
     3001 accaatctcc ttgccatatt tttccagcgc tgccggtgat atcatcgcgg tcgccggatg
     3061 ggcaagaata ttaataaacg ctgagaatgg ctgtttgagg gtaatcttta ccgttgtcgg
     3121 atcgatcgct tccgttttgg cgatattctt atacaggtta tagcgtttaa gatgattcgc
     3181 cggatcgctg gcccggtcca gattcgcttt caccgccgct gcgttgaaat cggtgccatc
     3241 ctggaattta atgccttccc gcaatttcac ggtgtaagta aggccgtcat cggaaacggt
     3301 gtaactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     3361 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt
     3421 ggtgaagttc gatcccaccg ccaccaccac ctctttggca gcgaatgcac aagatgccat
     3481 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     3541 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     3601 aatgccccgg cccgacgcat tgcaacgaga cggctgcaac ctcttcgcca cgcagatgaa
     3661 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     3721 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcggattt tcaaagaccg
     3781 cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     3841 tccgctcaac cacggccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     3901 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgagacgg
     3961 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4021 ggcgctgacc gccggaaaac tcatgtgggt aacgccaggc atgttccggt aacaggccca
     4081 cgcgctccag caaccacgca acgcgtgcag ccgcttcttt acctggcaat aaaccgtgta
     4141 cgcgcagcgg ttcgagaatc gaatcaccga tagtctgacg tgggtccagc gaagcgtaag
     4201 ggtcctgaaa aataaactga atatcgcggc gcaatgcctg aagtttaccg ggtgacaagg
     4261 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcaaca
     4321 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     4381 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     4441 acaaaccgct gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa acaggttcgc
     4501 catccaccac cgttttctgc tcgatggatg actcctgttt cgctggatgt tcaagcgata
     4561 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     4621 ctaacagcgc acgggtataa ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg
     4681 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacac
     4741 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     4801 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     4861 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     4921 agagttgatg cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     4981 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaatag
     5041 attcagcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc ggctcctgaa
     5101 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     5161 gttcaattac ttcgcgactg cgccgctgca acagcatttt atcgcactgc actaaaccgc
     5221 ccgcctgttc caacaggcgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     5281 cgccaacaat tgccagcgtc tcaccacgtt gcaggctaaa agagagattg cggaccgcag
     5341 ctattttctg ctgctcctgc ataaaggcaa tattcagatt ttcaaccgcc agcacatcac
     5401 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     5461 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     5521 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     5581 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     5641 gcggcgatat catatgccgc cagcgcgcgg atgaagactt cgcccgtacc ggtacaggaa
     5701 accgccacac tggcgttatt ggcatagcat ccggccccca ctaagggact atcgccaact
     5761 cgtccgggta atttattggt cattccgccc gtggacgtgg ctgccgccag attgccgtct
     5821 aaatccaacg ccacggcccc tacggtgccc attttctgtt tttcatccag tggcgcaccg
     5881 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca ttagttgttc ataacgcaaa
     5941 ggtgtggaga aaatctccgg cgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6001 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6061 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     6121 atcagacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     6181 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     6241 tcgcccgcta ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     6301 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     6361 gcaatgactg ctttgcccat agtattcgtc cattaagagt ggctcgccac ttttgctata
     6421 aatatcagaa tttttatgaa tgactcattc aatttttgaa tatagaaaga tggatatggc
     6481 atgtaaaggc aggagccagc gctacgcccg aggtggcata cagtctgtgg cgtttttctg
     6541 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     6601 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccgc
     6661 ccaggaaacg ctgccactgg ttcagtgttt tggccgtatt ctggcgagcg atgtcgtttc
     6721 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     6781 cgatattgcc tccgggcaac cgctgcccgt tgctggtaaa tcctttgccg gtcagccgta
     6841 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     6901 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     6961 tactgctgaa gcgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7021 tgcggttgtt ttcccggcgg ggactcgcct gactaccgct gaactgccag tgattgcttc
     7081 gctagggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     7141 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg ataccaaccg
     7201 tctcgccgta cacctgatgt tagaacagtt gggatgcgag gtaatcaact tagggattat
     7261 ccgcgacgat ccccatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     7321 ggtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     7381 agagctgggg gagatcgcct tctggaaact ggcgattaaa ccgggtaaac cgttcgcgtt
     7441 cggtaaactt tctaatagtt ggttctgcgg cctgccgggc aacccggttt cagcgacgct
     7501 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaata ccgccagcgg
     7561 cctgcccgcg cgccagcgtg tacgcacggc atcgcctctg aagaaatcgc caggacgcct
     7621 tgatttccag cgcggcgtgc tgcaacgcaa cgccgatggc gaactggaag tgacgaccac
     7681 cggacatcag ggttcacata tatttagctc ctttagcctc ggcaactgct ttatcgtgct
     7741 ggaacgcgat cgcggcaatg tggaagtggg cgaatgggtg gaagtagaac cgtttaacgc
     7801 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     7861 caaatcattc tgcgcggttt tgattttgac ggtcaggaag cattgaaaga ttctcgcgtg
     7921 ctggtcgtcg gccttggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     7981 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctccaatct gcaacgccag
     8041 acgctgcaca gtgatgccac ggtcgggcaa ccaaaggtgg aatccgcccg tgacgccctg
     8101 acgcggatca acccacatat cgcgattacg ccagtcaatg cgctgcttga tgacgctgaa
     8161 cttgctgcga tgattgcaaa acacgatctg gtgctcgact gtacggataa cgttgcagta
     8221 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     8281 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     8341 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     8401 ccgttaatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     8461 tatggcaaac cagccagcgg gaaaatcgtg atgtacgatg cgatgacctg tcagtttcgc
     8521 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaact gccacctcca
     8581 ggccagataa gacgcggcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
     8641 cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgccacagc
     8701 ggcatcaacc gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga
     8761 ttcacaccct gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgcggctaa
     8821 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
     8881 accgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
     8941 cagcgccgac agtagccctt gtgctgcgcc atataccgcg gttcccagcg tcggaatccc
     9001 ttccgctttt aacatcttaa tagctgccag cccctctgcg gtcaccggaa ctttcaccac
     9061 gatatccgca ataatagaac gcaatttacg tgcgtcattc accatccctt cagcagtggt
     9121 agccattacc tgggcaaaca gacgcccctg accgcccatc gcctcgtgaa gttgcggaag
     9181 cacaacctcc agcggttttt tacccactgc gataatgctt gggttagtgg ttacacccgc
     9241 cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag
     9301 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
     9361 tctgaacagt tgacgcacat caatccaact ttcattcgaa agtattttaa tgtttgaacg
     9421 aaacagagag gcaactatga ttttcaatat tcaacgctac tcgacccatg atggccccgg
     9481 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
     9541 ggaaagccgc gcccgcacgc aggatctgct gtatgaccca cgactctgtc tggaaggctg
     9601 tgagctgtgc gctaagaccg caccggaagt gattgagcgc gcgttgaatg gtttgcttat
     9661 tcatcgggaa aagttaaccc cggagcattt tacggcgcta accgactgct gcccgacaca
     9721 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcaa ccgttttgcg
     9781 cgataaaccg ttttacgatc gcagcggcgg cggcttaaca ctttctggcg gagaaccctt
     9841 tatgcagccg gaaatggcga cagcgctact gcaagccagc cacgaagccg gtattcatac
     9901 tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgtcctatat
     9961 tgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    10021 cggtaacgcc gccagggtgc tggataatct gaaaaaactc gccgcagcgg gcaaaaaaat
    10081 cattatccgc gtgccactga ttcagggctt taatgccgac gaaacctctg tgaaagccat
    10141 taccgatttt gccgccgacg agctgcacgt tagcgaaatt catttcctgc cctaccacac
    10201 gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    10261 tgatgcgcca gaactgctcg actttgccca gcagtatgcc tgccagaaag ggttaaccgc
    10321 gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag cgatcgcatt
    10381 aaagcgcaca aaaatgcgct ggtgcatatt gtgaaaccgc cggtctgtac cgagcgcgcg
    10441 cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg
    10501 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacatga tgaattgatc
    10561 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata taccgtctcc
    10621 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggttt tgcggtgagt
    10681 gaagagaaca aacgcgttct gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag
    10741 gatcgctgct acggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt
    10801 aaagcggagg gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctactg
    10861 ctggaaaaag gtcttgatgg tctgcgtgag aaagtggcgg aacgtcgctc gcgcatcaat
    10921 ctgacggttc tggaagatct gcacggcgag caattcctga aagcgattga tatcgtgctg
    10981 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgaaat ggccgcgacc
    11041 gaaacccgcg aaagccgtcg cgatgaactg ctgacgatag cagaaaactg cgatcttatc
    11101 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg
    11161 attttgcaga ttgaatctaa cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc
    11221 tatccatact atcgccgtga cgttgaactg aaccagacac tggatcgcga acacgccatc
    11281 gagatgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctca
    11341 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cgggcaaaat
    11401 ctggttgatg gtcaaccgat ggacgcggta aatccactct cttatgcgat cctcgaatct
    11461 tgcggtcgcc tgcgttcgac tcagcctaac ctcagcgtgc gttaccatgc gggtatgagt
    11521 aacgatttcc tcgacgcctg tgttcaggtg atccgctgcg gcttcgggat gccggcgttc
    11581 aacaacgacg aaatcgtcat cccggaattt attaaactcg gtattgaacc gcaggacgct
    11641 tacgactacg cagcaattgg ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc
    11701 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggt
    11761 cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc agcgggtaac
    11821 ttcaacaact tcgatgaagt aatggacgca tgggatacgc aaatccgtta ctacacgcgt
    11881 aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt acacgatatt
    11941 ctctgctcgg cgctggtcga tgactgtatt gagcgagcga aaagtattaa gcaaggcggc
    12001 gcgaaatatg actgggtttc tggcctgcag gtcggcattg ctaacctcgg caacagcctg
    12061 gcggcagtga agaaactggt atttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    12121 gcactggcgg atgacttcga cggtctgact cacgagcagc tgcgtcagcg gctgattaac
    12181 ggtgcgccta agtacggcaa cgacgatgat actgtcgata cgctgctggc tcgcgcttat
    12241 cagacctata tcgacgaact aaaacagtac cataacccac gctacggtcg tggtccggtt
    12301 ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccctt tggcgcgcag
    12361 actatggcaa caccggacgg gcgtaaagcg cataccccgc tggcagaagg cgctagtccg
    12421 gcctccggta ctgaccatct cggcccaacg gcggtcattg gctcagtagg taaactgcct
    12481 acggccgcga ttctcggcgg cgtgctgctc aaccagaaac tgaatccagc aacgctggag
    12541 aacgaatctg acaagcagaa actgatgatc ctgctgcgca ccttctttga agtgcataaa
    12601 ggctggcata ttcagtacaa catcgtttcc cgcgagacgc tgctggaggc gaaaaaacat
    12661 ccggatcagt atcgcgatct ggtagtgcgc gtcgcgggct attccgcctt cttcaccgcg
    12721 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatcttct
    12781 cagcccggcg gcggcgtcgc cgggctaaat cattcacatc atcaatttca gcctcgcttt
    12841 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    12901 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagacatgg acggtacttt
    12961 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa
    13021 aaagcgcggc attgagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    13081 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    13141 acatggcaag cagttgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    13201 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    13261 cagcgaaaat gcccccgaag cattcgtcgc actgatggca aaacactacc atcgcctgaa
    13321 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    13381 ggatgaacaa atcccgttag tgatcgacaa actgcacata acgctggatg gcattatgaa
    13441 acccgtcacc agtggttttg gctttatcga cttgattatt cccggtctac ataaagcaaa
    13501 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    13561 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgagcaa
    13621 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    13681 cgcgctgaat gtgattcagg cggtgctgga aaataaaccg ccgtttaatc tttgattatc
    13741 accaattcag tttattgaga aagtgcatgt cattataccg gatacggcgt gaccgcctta
    13801 tttggcatac aaaatcatga aaattcaata tattgcgata cactaccagg cctgataagc
    13861 gtagcgcacc aggcaatctt gcgtttgtca tcagtttcag tagccctgac catatcaggg
    13921 ctactgataa taacttactc gatcccttta ctacgcaggt aatcttcgta attaccgcta
    13981 aagtcgatca cgcgttccgg ggtgatttcc agaatacggg tcgccaggga gcttacaaac
    14041 tcacggtcgt gagaaacaaa gatcagcgtg ccctgataca gttccagcgc catgttcagc
    14101 gactcaatgg attccatatc caggtggttg gtcggttcgt ccatgatcag aatgttcggc
    14161 ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga aagcacttta
    14221 gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatact gcgcaccgcc
    14281 tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt cagatcattt
    14341 tcaaactcat attcgtggtc ctgagcgtag taaccaatgc gcgcgttttc agaccatttt
    14401 acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt tgatttaccg
    14461 acgccgttgg tacccagtac cgccagtttt tcacccactt ccagcagcag gttgagattt
    14521 ttaaacagtg gaccgttatc aaacccttta gtcagacctt ccacttctag cgcgttacgg
    14581 aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga agctttcacc
    14641 tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag
    14701 gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc tttcttcttc
    14761 gcgttatcag ccagcagacg ttcacgcgcc tgagtcgccg ccgtcatgta ctcatcgtag
    14821 ttgcccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt acaaaccatg
    14881 ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc gttcagcacc
    14941 tgttccagcc agcgaatggt gtcgatatcg aggttgttgg tcggttcgtc gagcaggaga
    15001 atgtcgggat cagcaaacag cgcctgagcc agcagcacac gcagcttcca gccaggagca
    15061 acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag caacagttca
    15121 ccagcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc cagatcggcc
    15181 actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc acgctcctgc
    15241 ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt gaactcttca
    15301 aaggcaaact gatcctgacg cagcttacca atgcgttcgt tgggatcgag ggaaacgtta
    15361 cccaacgtcg gctctaagtc gccgccgagg atcttcataa aggtggattt accactaccg
    15421 ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat gttttcaaac
    15481 aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg cgtatcctga
    15541 aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc tgctcctcgc
    15601 ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc tgatttcact
    15661 gtgagctgga atgaacttat aatgcgcttc cagtactcta atattctcaa cccaatggcc
    15721 tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat tcgcagcggc
    15781 cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc tgccaaccga
    15841 cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta acactcagcc
    15901 gctggagtat tttgccgccg agtaccagat gggactttcc aatatgatgg aagcgaaccc
    15961 gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc agcagctgat
    16021 cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc gtctgtatta
    16081 ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc agttaggcaa
    16141 agacacgcct atcaactgga ccaccaaagt tgagcgtaaa aaagcaggcc cgacctggac
    16201 gccgaccgct aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc cggcggtcgt
    16261 tccggctggt ccggataacc cgatggggct gtatgcactc tacatcggtc gcctgtacgc
    16321 tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg
    16381 tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta cccgcgtaca
    16441 gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt atattgaagt
    16501 ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaattg tgccaattac
    16561 cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgaccagg ttgttctgga
    16621 tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgtctg aattaatctt caaaacgtaa
    16681 agcaaaggcg gactcataat ccgccttttt aatttgacag accttatccc gccgggatca
    16741 taaccaacaa aacatatccc accagcgccg cccacaacag catcggtatc gaataaagat
    16801 gaaaacgcca ccagatacgg cgatcgttcg ccatccgtaa cgcaatcaaa tttgccagcg
    16861 atccgggtaa taaaccaaag ccacctacgt ttaccgccca tgccagtaat aaagacggtg
    16921 gcacataatt gagcagcaaa atggtactcg gcacgttact gatcacctgc gataaaccga
    16981 tcgccgttag ccataatccg ggttcggata aatgactcac gttacttaac accccctgca
    17041 aaactggcag ttgggttagt aaatggacgt cgataaacat cgccataaac accagcagca
    17101 gcgtccagtc cacactgagc accacgcgac gcgccagcag cgcaaagccc gccgccacaa
    17161 tcaccagtcc ccacagctct tgttttaact ccagcgccgt cagaaagacg atatacagcc
    17221 ccagacaact ccacaccagc cgcggtttcc actccggcgt ttgccctcct gtatggtact
    17281 gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc atcattgcgc
    17341 cagccagcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa cgtccccaga
    17401 taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcattg actgccagcg
    17461 cctcaaaaat aatcagccga ttaaccggga tctcacacag tctttttagc gtgatagtca
    17521 gcggaacaac tataaacagc gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg
    17581 ccagtaccat aaacatcgcc agccgacgct ccgtagcaaa acggcgcacc attttgcgcc
    17641 ccagcacatc aaaataaccg cttaactcga cacctttagt cagcagcatc aggccgctta
    17701 aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca aagggaacga
    17761 aaaaacttaa tccgatgcca acaagaatta ataactgaaa aaaacgatcg ccttgcagcg
    17821 tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac gaaaggcatc
    17881 cagcgtctct tcactaacat ggtgctccat accttccgcg tcgcgacggg cgatttccgg
    17941 actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc ggctttcctg
    18001 cgccagcttc tctccttctg ccgttaaaaa cacgccacgc caggggatca tttcaatcag
    18061 ccccatggtt gccagtcgct taagcatttt agccaccgtc ggttgcgaaa cccccagacg
    18121 agcagccatg tctacctgac gagcttcccc cacttccctg atcaagtcag aaatcagctc
    18181 aacataatca tcaataagct cgcgccgatg cgcctctctg acctggcgga acccttcaac
    18241 gtgctcttcc acgttcacta actgcgtcac tttttttgct gttggcgtac ctgcgcgacg
    18301 actcattatg cttcctcatt atggtgacgc atacatagcg ttctcatttc gtgcgcacat
    18361 tgtaaaccag agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct atataatata
    18421 gcctgtgcta tatctgtatg taatgcaatc atccctcaag gatcgatggg attagcaagt
    18481 caggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta
    18541 aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa ccgcagcagg
    18601 ataaaccttc cgataaatag cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag
    18661 gcacaacgcg ttttggtctg aaaaacccca ctttttgtcg gatttgcaat ccccttcgca
    18721 aaagatttgt tcgtcggtag ttgacctgaa cagcagctcg ctctatcttc ttgcagccct
    18781 gcgtatattg cggctcgcgg atgcggaccc ctttcccctc ttcacgcact cttgcaggta
    18841 ttgacccttg acgccagggt aagcacatgg cgtttgttac gatagtggca tatgaattta
    18901 accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac tgcgttctac
    18961 tttttacagt cgctattaat taacctcggc ttaggttacc ccttcagttt gctctacacc
    19021 gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt
    19081 ctggtcggta tcagttcgct ggtggcggct tgttatttcc cttttgctca ggcctacggc
    19141 gcgcctaact tcaatacatt gctggcattg cactccacca atatggaaga gtcgaccgaa
    19201 atcctgacga tttttccgtg gtacagctac ctggtcggca tatttatttt tgcgctcggc
    19261 gtaatagcaa tcaggcgaaa aaaagagagt gaaaaagcgc gctggaatac cttcgacagc
    19321 ctgtgcctgg tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg
    19381 ggcggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc taaggatgtc
    19441 atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact ttccggaatg
    19501 aaagatacct ggacggtcac tgccgttaag ccgaagtatc agacctatgt ggtggtgatc
    19561 ggagaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga caataccccg
    19621 tttgccagca gcgttaacgg tttgatattt gctgactaca ttgccgccag tggctccacg
    19681 cagaaatcgc ttggcttaac gctcaatcgc gttgtcgatg gcaaaccaca gtttcaggat
    19741 aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc caaccagggt
    19801 caaatcggcg aatacgatac cgccatcgcc agcatcgcca aacgagcaga tgaagtgtac
    19861 ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc gttactggat
    19921 atgaccgctc aagtgctggc gcaagagcac tcgcaaccgc agctgattgt tctacatctg
    19981 atgggctcgc atccgcaggc ctgcgaccgg acacaaggaa aatacgaaac ctttgtgcaa
    20041 tcgaaagaaa cgtcgtgcta tctctatacc atgacgcaaa cagacgattt actgcgcaag
    20101 ctgtacgatc agttacgcaa cagcggcagc agcttctcgc tggtttactt ttctgaccac
    20161 ggtctggcct ttaaagagcg cggcaaagac gtgcaatacc ttgctcatga tgataagtat
    20221 cagcaaaatt tccaggtgcc ttttatggtc atttccagcg acgataaagc gcatcgggtg
    20281 attaaagccc gccgctcggc caatgacttc ttaggctttt tctcgcagtg gacgggaatt
    20341 aaagcgaagg aaataaatat caaatacccg tttatatctg aaaagaaagc cgggccgata
    20401 tacatcacca acttccagtt acagaaggtg gattacaacc atctcggaac cgatattttc
    20461 gacccgaaac cttaaaacaa aaaaatccgc cccgagaggc ggatttttat atcaccgaag
    20521 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    20581 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    20641 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    20701 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    20761 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    20821 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    20881 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    20941 tgcgtaaccg ccagttacag tagaagtcgc agctacggaa gtacctgcgg tgaaagccag
    21001 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    21061 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttatgat
    21121 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    21181 aatcactttt tcagagaact gcgtaagtat tacgcatatt ttccctgtca ttcatccaga
    21241 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    21301 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    21361 tacttaggtt tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    21421 ctggttacca atagtcatat tgctcgttgc catggcgtct attcagggtg gagcctcgtt
    21481 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    21541 aggcacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    21601 acgattaccg ctgttgtttt acggcgtttc gctgggtgga atgaattatc ttttttatct
    21661 ttctattcag acggtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    21721 ggtggcactg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    21781 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    21841 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    21901 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    21961 cgtcccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    22021 ggggctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    22081 cacccgtttg ccaacacgca catttggtac gctaatgagc atggaaccgg ctctggctgc
    22141 cgtttccggg atgattttcc tcggagaaac actgaccccc atacagctgc tggcgctcgg
    22201 cgctatcatc gccgcttcaa tg
//
LOCUS       DAPQZD010000071        21817 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPQZD010000071 DAPQZD010000000
VERSION     DAPQZD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.071"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            125..667
                     /gene="zapC"
                     /locus_tag="RO454_002631"
     CDS             125..667
                     /gene="zapC"
                     /locus_tag="RO454_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDY0934446.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(664..1773)
                     /gene="ycbX"
                     /locus_tag="RO454_002632"
     CDS             complement(664..1773)
                     /gene="ycbX"
                     /locus_tag="RO454_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDY0934447.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            1871..3979
                     /gene="rlmKL"
                     /locus_tag="RO454_002633"
     CDS             1871..3979
                     /gene="rlmKL"
                     /locus_tag="RO454_002633"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDY0934448.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            3991..5898
                     /locus_tag="RO454_002634"
     CDS             3991..5898
                     /locus_tag="RO454_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0934449.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6028..7281
                     /gene="pqiA"
                     /locus_tag="RO454_002635"
     CDS             6028..7281
                     /gene="pqiA"
                     /locus_tag="RO454_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDY0934450.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7286..8926
                     /gene="pqiB"
                     /locus_tag="RO454_002636"
     CDS             7286..8926
                     /gene="pqiB"
                     /locus_tag="RO454_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDY0934451.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            8923..9486
                     /gene="pqiC"
                     /locus_tag="RO454_002637"
     CDS             8923..9486
                     /gene="pqiC"
                     /locus_tag="RO454_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDY0934452.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            9742..9909
                     /gene="rmf"
                     /locus_tag="RO454_002638"
     CDS             9742..9909
                     /gene="rmf"
                     /locus_tag="RO454_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDY0934453.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9979..10497)
                     /gene="fabA"
                     /locus_tag="RO454_002639"
     CDS             complement(9979..10497)
                     /gene="fabA"
                     /locus_tag="RO454_002639"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDY0934454.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(10566..12326)
                     /locus_tag="RO454_002640"
     CDS             complement(10566..12326)
                     /locus_tag="RO454_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDY0934455.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12512..12964
                     /gene="matP"
                     /locus_tag="RO454_002641"
     CDS             12512..12964
                     /gene="matP"
                     /locus_tag="RO454_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDY0934456.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13040..14092)
                     /gene="ompA"
                     /locus_tag="RO454_002642"
     CDS             complement(13040..14092)
                     /gene="ompA"
                     /locus_tag="RO454_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDY0934457.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14448..14957)
                     /gene="sulA"
                     /locus_tag="RO454_002643"
     CDS             complement(14448..14957)
                     /gene="sulA"
                     /locus_tag="RO454_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDY0934458.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15176..15805
                     /gene="sxy"
                     /locus_tag="RO454_002644"
     CDS             15176..15805
                     /gene="sxy"
                     /locus_tag="RO454_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDY0934459.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15768..17930)
                     /gene="yccS"
                     /locus_tag="RO454_002645"
     CDS             complement(15768..17930)
                     /gene="yccS"
                     /locus_tag="RO454_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDY0934460.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17940..18386)
                     /locus_tag="RO454_002646"
     CDS             complement(17940..18386)
                     /locus_tag="RO454_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDY0934461.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18509..20563
                     /gene="helD"
                     /locus_tag="RO454_002647"
     CDS             18509..20563
                     /gene="helD"
                     /locus_tag="RO454_002647"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDY0934462.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20595..21053)
                     /gene="mgsA"
                     /locus_tag="RO454_002648"
     CDS             complement(20595..21053)
                     /gene="mgsA"
                     /locus_tag="RO454_002648"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDY0934463.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21149..21811)
                     /locus_tag="RO454_002649"
     CDS             complement(21149..21811)
                     /locus_tag="RO454_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDY0934464.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         5553 a         5258 c         5805 g         5201 t
ORIGIN      
        1 ccctggttgt tttatattca tcgctgttgc ttatttagac attttgtact tttattgttg
       61 aggttattaa gcgaagcgac aatggattgt gtcgctgcgg ttttataagg ttggggagtg
      121 gcagatgcga attaaaccag acgataactg gcgttggtat tacgatgaag agcacgatcg
      181 aatgatgctc gatttagcca atggtatgct atttcgctca cgttttgcgc gcaaaatgtt
      241 gactccagat gccttttctc ccgcaggttt ttgcgttgat gacgccgcgc tctatttctc
      301 ttttgaagaa aagtgccgcg attttaattt atctaaagaa caaaaagccg aactggtgct
      361 gaatgcactg gtagcaattc gttatctcaa accgcaaatg ccgaaaagct ggcattttgt
      421 atctcatggt gaaatgtgga tgcccatgcc gggcgatgcg gcctacgttt ggttgagcga
      481 cactcacgag caagtgaatt tactggtcgt tgaatctggc gagaatgccg cgctatgtct
      541 actggcacaa ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg acgccattaa
      601 aatcatgaac gacaggctga aaccgcaggt taatgttgac agcttcagcc tcgaacaggc
      661 agtctaacgc gccagcttaa gcgcagtctt cggtacacaa ctacagcaaa gaatagtgcc
      721 atcatcgcct gttgctgatt tttttagcgg cgtgacttcg ccttctaaaa gctgaacacg
      781 gcaacttcca caaatgcctg cgcggcaaga ataagggata cgaattccct gattttctaa
      841 ttgctccagc agcacctgtt ggttatttcc acgaaatgcc tgtccctgcc agtcaatatc
      901 tacgtttgcg tccggttgtt gcgtgatgtt ggcagtatca tcagcggcac ctgcgccgta
      961 aattttagct ggagccgttg ccagaatttc cacctcatcg ccaacgcgaa tcacgccgct
     1021 attacgggca attaaattct gaccaaaatc gacatcgccg ttatcctggg cagtacggaa
     1081 agattgcagc gtttttaatg gttcgtctgc cggatgtttt tgcccttttt ctgggctgac
     1141 ggtggtgaaa atacagcggc tacaaggctt aaccacatca aacacgacat caccaatgcg
     1201 aatcactttc cagctatctt cttcccaggc cgatgcaccg gaaaccacca gattggggcg
     1261 gaattgctcc atttttacac tggccggaca acgttgttgg agagcacgta acgaggcctc
     1321 gttagcaaga aggtaaggat agccatcagc aaatgacagg ggtacagtgt tgtggcgttt
     1381 cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc acttcgcggg agaaaaatcc
     1441 acttagccat ttgttgatcg catctggcgc aattcgcgcg gtaaaatgtg tgccccaaac
     1501 ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc tactgccatc
     1561 tggtgcggtg agatgcaagc catcatgcac gggtgaagga gtaaaccgta ccattagcgg
     1621 aaactggcga gcggtaataa acgtaccgtc aggttccgtg atcataaaga tgcgatcgaa
     1681 ggccagacca ctgacatctg ccagggtatg tgtaagacca atgccgcgca tcgatttaac
     1741 aggatgaata aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg aaaataagcc
     1801 ctcaacttta tgacatgacg cgcttattag ctataatgcg caacaatttt cttagagtaa
     1861 aagtgacgat atgaattctc tgtttgccag tacggcccgt gggctggaag agctgttaaa
     1921 aactgaactg gaaaacctgg gggccgttga atgccaggtg gttcagggtg gggtccattt
     1981 caagggcgac acacggcttg tttaccagag cctgatgtgg agccgcctgg cctcgcgtat
     2041 tatgttgccg ctgggcgagt gtaaggttta cagtgattta gacctctatc tcggcgttca
     2101 ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc ttcgctgtcc atttcagtgg
     2161 tttaaatgac accattcgca acagccagta cggcgcgatg aaagtgaaag acgcgatcgt
     2221 cgatgctttc acgcggaaaa atctgccacg tcccaatgtt gatcgcgatg cgccggatat
     2281 tcgcgttaac gtctggctgc ataaagaaac cgccaatatc gcccttgatc tcagtggtga
     2341 tggcttgcat ctgcgtggct atcgtgatcg tgctggtatt gcaccgatca aagaaaccct
     2401 ggcagccgcg attgtgatgc gatccggctg gcagccagga acaccactgc tcgatccaat
     2461 gtgtggttct ggtacgttgc tgattgaagc agcgatgctg gcgaccgacc gcgcaccggg
     2521 cttgcaccgt ggccgttggg gctttagcgg ctgggcgcag catgatgaag ctatctggca
     2581 ggaagtgaaa gcggaagcgc aaactcgcgc ccgtaaaggc ctggcagagt atagctctca
     2641 tttttacggt tcggacagcg acgcccgggt gattcaacgt gcgcgcacta acgcccgtct
     2701 tgcggggatt ggtgaactga tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc
     2761 gctgccgaaa gggccgtacg gtacagtgtt gagcaacccg ccatacggtg aacgtctgga
     2821 cagcgaaccg gcgctgattg cgctgcatag tctgctgggc cgaatcatga aaaaccagtt
     2881 cggtggctgg aatctctctt tgtttagtgc ctcgccggat ctgctaagct gcctgcagct
     2941 gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg gactgcgtac agaaaaacta
     3001 ccacgttgcc gaaagcacac cagacagcaa accggcaatg gtagcggaag actacgccaa
     3061 ccgcctgcgt aagaacctca aaaaattcga gaagtgggca cgtcaggaag ggattgaatg
     3121 ttatcgcctg tatgatgccg atctgccgga atataacgtt gccgttgacc gttatgccga
     3181 ctgggtggtg gtgcaggagt atgcgccgcc aaaaactatt gatgcgcaca aagcgcgtca
     3241 gcgtctgttc gatattatcg ctgcaaccat ttcggtactg gggattgcgc caaacaaact
     3301 ggtgctgaaa acccgtgaac gccagaaggg caaaaatcag taccagaaac tgggcgagaa
     3361 gggcgagttt cttgaagtta ccgaatataa cgctcacttg tgggtgaact tgacggatta
     3421 cctcgatacc ggtctgttcc tcgatcaccg tatcgcccgt cgtatgctcg gtcagatgag
     3481 caaaggcaaa gatttcctca acctgttctc ctataccggt agcgccaccg tgcatgcggg
     3541 attaggcggc gcgcgcagca ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc
     3601 agaacgcaac ctgcgtctga atggcctaac cgggcgtgcg catcgcctga ttcaggccga
     3661 ttgcctggcg tggctgcgtg aggcaaatga acagttcgat ctgatcttta tcgatccgcc
     3721 aaccttctct aactcaaaac gaatggaaga tgcgtttgat gttcagcgcg atcatctggc
     3781 gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa
     3841 caaacgcggc ttccgtatgg atctcgacgg cctggcgaaa ctgggactga aagcacaaga
     3901 aattacgcaa aaaacgctct cccaggattt cgcccgtaac cgccagatcc acaactgctg
     3961 gctgattacc gcagcctgaa aggaatagta atgtcattaa tcagtatgca tggcgcatgg
     4021 ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga agataacgaa
     4081 cgtgtttgtc tggtgggtcg taacggcgca ggcaaatcga cgttaatgaa gatcctcaac
     4141 cgtgaacagg ggctggatga cggtcgcatt atttacgagc aagatttgat tgtagctcgt
     4201 ctgcaacagg atccgccgcg taacgttgag ggtagcgttt atgatttcgt tgccgaaggc
     4261 attgaagaac aagcggaata cctgaaacgc tatcacgata tttcgcgcct ggtgatgaac
     4321 gacccgagcg agaaaaatct caacgaactg gcgaaggttc aggaacagct ggatcaccac
     4381 aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg attagatcct
     4441 aatgttgcgc tgtcgtcgct ttccggtggc tggttgcgta aagcggcatt aggacgcgcg
     4501 ctggtgagca atccgcgcgt attgctgctc gacgaaccga ctaaccacct ggatattgaa
     4561 accatcgact ggctggaagg gttcctcaaa accttcaatg ggacaattat tttcatttcc
     4621 cacgaccgtt cgtttatccg caatatggcg acgcgcattg ttgatctcga tcgcggcaag
     4681 ctggtgacct atccagggaa ttacgaccag tacctgctgg aaaaagaaga agccctgcgc
     4741 gtggaagaat tacaaaatgc cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc
     4801 cgccagggga tcaaagcacg tcgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca
     4861 atgcgtcgtg agcgtggtga acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa
     4921 gaagccagcc gctccggtaa gatcgttttc gaaatggagg acgtttgcta ccaggttgac
     4981 ggtaagcaac tggtgaaaga tttttctgcc caggttctac gcggcgacaa aattgccctg
     5041 attggtccga atgggtgcgg caaaaccacg ctgctgaaac taatgctcgg tcagcttcaa
     5101 gcagacagcg ggcgtattca cgttggcacc aaactggaag tggcttattt cgatcagcac
     5161 cgcgcggaac tggatcccga taaaacggtg atggataacc ttgccgaagg taagcaagag
     5221 gtgatggtta acggcaagcc acgccacgta ttgggctatt tgcaggactt cctgttccat
     5281 ccaaaacggg cgatgacgcc ggtacgtgcg ctttctggcg gtgagcgtaa ccgcttgttg
     5341 ttggcgcgtt tgttcctcaa accaagcaac ttattgattc ttgacgaacc gaccaacgat
     5401 cttgatgtcg aaacgctgga actgctggaa gaactgatcg acagctatca gggcacggta
     5461 ttgctggtta gccacgatcg tcagtttgtc gataacaccg ttacagaatg ctggatcttc
     5521 gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa
     5581 gaacagtatg tggcgctcaa acagcctgcg gtgaaaaaaa gcgaagaagc cgccgcgcca
     5641 aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct ataaattgca gcgtgaactg
     5701 gagctgctac cgcaattgct tgaagatctg gaggcgaagc tggaagcttt acagacgcaa
     5761 gtggcggatg cttccttctt cagtcagccg catgagcaga cgcaaaaagt actcgctgat
     5821 atggctgctg cagagcagga gctggagcaa gcctttgaac gctgggagta tcttgaagcg
     5881 ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac agagactgct gacaacggta
     5941 aaattgcctg atgtactggg tttatcaggc atgaaaacca acactttgtt agtcatctgc
     6001 aaaatagcgc atcattaagg agtaccaatg tgcgaacatc atcatgccgc gaagcacatc
     6061 ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg
     6121 gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg atgcccctcg gcagcgtccc
     6181 accgcctatg cgttggctgc actgttcatg ctgttgctgt ccaacttgtt tccttttgtg
     6241 aatatgaacg ttgcaggagt taccagtgaa attacattac tggaaattcc cggcgtgctt
     6301 ttttctgagg actacgccag cctcggcacc tttttcctgt tgtttgtgca actggttccc
     6361 gcgttttgtc tgataaccat tctgttactg gtgaatcgcg cggaattacc ggtccgttta
     6421 aaagagcaac tggcacgggt gctttttcaa ctcaaaacct ggggaatggc ggagattttc
     6481 ctcgcgggtg tgctggtcag tttcgttaaa ctgatggctt acggcagcat tggggtaggc
     6541 agcagctttc tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt
     6601 gatcgtcgct ggttatggga cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa
     6661 ccaggcgtca cggggatacg ccaggggctg cgttcgtgtt cctgttgtac ggcaatcctt
     6721 cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac
     6781 agcctgcaat ggacactcgc gctgcttgta acgtctatca tgctgtatct tccggctaat
     6841 attttgccca tcatggtgac ggatttatta ggctcgaaga tgccgtcgac gattctcgct
     6901 ggggtcattc tgttatggag cgaaggctct tatcccgtcg ctgcggtgat ttttctggcc
     6961 agtattatgg tgccaacgtt aaagatgatc gccattgcgt ggctgtgttg ggatgccaaa
     7021 ggacacggca agcgcgacag tgaaagaatg catttgattt atgaagttgt tgagtttgta
     7081 ggccgctggt cgatgattga cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg
     7141 ggaggtttaa tgagtattta tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata
     7201 atgacaatgt tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa
     7261 tcagagcatg aggagtcctg acagtatgga atctaataat ggggaagcca aaatccagaa
     7321 agtgaagaac tggtctcccg tgtggatatt tcctatcgtc acggcgctca ttggggcctg
     7381 ggttcttttt tatcattaca gccatcaggg accggaagtg accctgatca ccgcgaatgc
     7441 ggaaggaatt gaaggtggca aaaccaccat taaaagccgt agcgttgacg tcggcgtggt
     7501 tgaaagcgcc acactggctg atgatttgac gcacgttgaa atcaaagcgc ggctgaattc
     7561 cggtatggaa aaattgctgc ataaagatac cgtcttttgg gtggtgaaac cgcagattgg
     7621 tcgagaaggg attagcggcc tgggaacgct gctgtctggg gtttatatcg aactgcagcc
     7681 aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg gactcgccac cgttggcccc
     7741 gcctgatgcg aaaggtatcc gtgtgcttct cgatagcaaa aaagccgggc agctctcgcc
     7801 aggggatccg gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca gcactttcga
     7861 tacgcaaaaa cgcaatatca gctatcaact gttcatcaat gcaccttatg accgactggt
     7921 gaccagcaat gttcgtttct ggaaagatag tggcattgcg gttgatctga cgtcagcggg
     7981 aatgcgtgtg gagatgggct cattgacaac gctgcttagt ggcggtgtca gctttgatgt
     8041 gccggaaggt ctggatttag ggcagccagt ggcaccgaaa acagctttcg ttttgtatga
     8101 tgatcagaag agcattcagg attcgttgta caccgatcac attgattatc tgatgttctt
     8161 taaagattcg gtacgcggtc tgcaaccggg agctccggta gaattccggg gtattcgcct
     8221 gggtaccgta agcaaagtgc cattctttgc gccgaatatg cgtcagacat ttaacgatga
     8281 ttaccgtatt ccggtactga ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga
     8341 aaatgcggat gttgttgagc accttggcga attgttgaaa cgtggtttac gcggatcgct
     8401 gaaaaccgga aacctggtca ctggcgcact gtatgttgat ctcgatttct atccaaatac
     8461 gcctgcaata accggtattc gtgaatttaa tggttatcag attatcccga ccgtcagcgg
     8521 cggcctggcg caaatccagc aacgactgat ggaagcgttg gataagatca acaaactgcc
     8581 actgaatccg atgattgaac aggcaaccag tacgctttct gaaagtcagc gcacaatgaa
     8641 aaacctgcaa acgacgctgg atagcatgaa caagatcctc gccagccagt cgatgcagca
     8701 gttaccgacg gatatgcagt caacgttgcg tgaattgaat cgcagtatgc agggcttcca
     8761 gcctggttcc gcagcctaca acaagatggt ggcggatatg cagcgccttg atcaggtgtt
     8821 gcgagaactg caaccggtgc tgaaaacgct caacgagaag agtaacgcgc tggtatttga
     8881 agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg
     8941 acgattgcgg cactgtggct ggccggatgc agctccggtg aaattaataa aaactattac
     9001 cagttacctg tggtgcagag cggtacacaa agcaccgcca gccagggtaa tcgcctgtta
     9061 tgggttgagc aggtcgctgt tcctgactat ctggcgggga atggtgtggt ttatcaaacc
     9121 agtgatgtga agtatgtgat tgccaacaac aacttgtggg ccagcccgtt ggatcaacag
     9181 ttgcgcaaca ccctggttgc caacctgagt acgcaactgc ccggctgggt ggttgcctcc
     9241 cagcctctgg gaagcgccca ggacacgctc aatgttaccg tcacggagtt taacggtcgc
     9301 tatgatggca aggtcattgt cagtggtgag tggctgttga accaccaggg acaactgatc
     9361 aaacgtccgt tccgtctgga aggagtgcaa actcaggatg gttacgatga gatggttaaa
     9421 gtgctggccc gtgtctggag tcaggaagcc gcttctattg cgcaagagat aaagcgtcta
     9481 ccttaatcat aaagatttgt aaatataacc gtctccggta tgttgcctga ggcggttttt
     9541 ttgtttctaa cgtgcggaaa aagttgttcc tcttcacatt ttttgtacaa ccgacatgcc
     9601 cgtgtagctc acaaatatga cagtggcgtg aattttgcgc attgacggca gttatgattc
     9661 gcggtattgc ttaactgtgg ttgcatattt agtaatcact gttttctttt ccaccagaaa
     9721 ccagtatgag ggaaacgagg catgaagaga caaaaacgag atcgcctgga acgggcacat
     9781 caacgtggtt atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg
     9841 ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag ccatggcgga cagggtagta
     9901 atggcctgat tctgtctctt taaaaagaaa cctccgcatt gcggaggttt cgccttttta
     9961 tactctgtct gattattatc agaaggcaga cgtatcctgg aacagaccga ctttcaggtc
    10021 gttggcggta tagatcaaac gaccatcaac cagcacttcg ccatcagcca ggcccataat
    10081 cagacgacgg ttaactatgc gtttaaagtg aatacgatag gtcacttttt tcgccgtcgg
    10141 cagcacctga cctgtgaatt tcacttcacc tacgcccaac gcgcgacctt taccttcgcc
    10201 gcccagccag ccgaggtaga accctaccag ctgccacatt gcgtccaggc ccaggcatcc
    10261 cggcataacc ggatcaccaa tgaagtggca tccgaagaac cacagatccg gattgatatc
    10321 cagctctgct tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac
    10381 acggtccatc atcagcatgt ttggtgctgg caattgcggg cctttagcgc caaacagttc
    10441 accgcgacca gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt
    10501 ctctgtaagc cttattttat tgaagcacgc aggatagcta acacgtgtac gctgaacaag
    10561 tccgatcagt tcggaataaa ccagttcagc caacgtaatg gccatggaaa acggtgacgt
    10621 ccttcctgtt gcgatgcttg tgcgatacgt tcctggatgg tttgcatcag cgtcgtctgg
    10681 ccttcgccat cccacaccag atttaataat aacggcagtg cgtcagtcac atcgtctact
    10741 gcccagatgg tgaatttgcc ttcttctacc gctttcacca gttcactgtg aagacttaaa
    10801 tggcgaacgt tagcagtggg gataatgaca ccttgtttcc ccgttaactc acgttgctgg
    10861 caaatagcaa agaagccttc gattttctca tttaaaccac cgactggctg ggcgcgaccg
    10921 aactgatcga ctgaacctgt gatagcgata ctctgattca ccggcacatc ggcgagggcg
    10981 ctgatcagag cgcagagttc agccatcgag gcactatcgc catccacttc actgtatgac
    11041 tgctcaaatg tcagcgatgc tgagaagggg atctgttgct caagctgtag ttccgacatc
    11101 aggaacgctt gcatgatcat catccctttc gcatggatat tgccgccaag ctccgctttg
    11161 cgttcgatgt cggtgaattc accatcgcca atatgcacaa cgcagctaat gcgagaaggt
    11221 tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt aatttgcccg
    11281 atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa ggatctcatc ctgcatgcgt
    11341 tcagcgagga aaccttcgcg ccattcacgc tgctgtagca ttaagtttag ctgctcgccg
    11401 gagaaggtgt cgccatcaca cagggaggcg acctcttgac actggcggag gatccactgc
    11461 gggctaagcg gaagtgtttc ttgttcacca gtgtagcgtg ctgcttcgcg gataagtacc
    11521 ggccaggcat ccgctcccgg tgctggtaag tgattatgtc tggcggtaaa tgtcacccag
    11581 cgacaccact ggcttactga ctccgcatcg acaatctgca gagtatcttc aaattcgcta
    11641 taaatagcct gctctgaaag ctctggctcc atctcctgga aatcagccaa tgattcgcgt
    11701 tcgcctacca gaatgacttt cagcttcaat ggcatcgaag gcacagagac ggggagaggg
    11761 cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc
    11821 atccacagca gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat accgccattt
    11881 gcctgatgca ccagaccagg ctgcagggta atatcgccat taaactgacg caggcagcca
    11941 aagagttgct ctgcttctac ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta
    12001 tcatctgcac tcactgcgtg acgtaagcgg atggtgtggc cggaaacctc atagtgaccg
    12061 cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg catcagcaat aagattcaga
    12121 tactcagact cttccggggc cttcgccagc ataaaagagg aggatgctcg cgtatgcagc
    12181 aactgctcca gcgcaaattg cagtcgcggt tgagtatcac tgaataaagg atcgttttcg
    12241 tcaatcaaat gtggctgagc aaatatttcc tgatagctat cggtatcagg aaccaggtca
    12301 cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg
    12361 attataccgt aaccggcact gcagcacacg tagaaagcaa cgacaatacg cctggcatgg
    12421 gcgttaaagc tcacaggatg gagattcttt tcttcactgg cctaaaaagc tgatattctg
    12481 taaagagtta cacggtaaca ttgagatcgc tatgaaatat caacaacttg aaaatcttga
    12541 aagcggttgg aaatggaagt atctggtgaa gaagcaccgc gaaggggagt taatcacccg
    12601 ttacatagaa gccagtgccg cccaggaagc cgtcgatgag ttgctctcgc tggaaaatga
    12661 acccgtgctg gtaaatggtt ggattgacaa gcatatgaat ccggaactgg tcaatcgcat
    12721 gaagcagacc attcgggcca gacggaagcg ccattttaat gcggaacacc agcatacgcg
    12781 caaaaaatcg atcgatctgg aatttatcgt ctggcaacgt ctggctggcc ttgcgcagcg
    12841 tcgcggtaaa acgctttctg aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga
    12901 gaaatacgcg aataaaatgt cttctttgaa gcaggatctg caggcattgc tgggtaagga
    12961 ataactgtca aaagtcagtt caatttacgc aaggcgaaaa aaaaccccgc agcagcgggg
    13021 ttttttacca gacgagaact taagcctgcg gctgagttac aacgtctttg atgcctttaa
    13081 cttcgatctc tacgcgacga tccggagcca ggcaatcgat cagtgcagca cgctgtttca
    13141 cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagattt
    13201 tgtctgccgg gataccttta gagatcaggt aatcaacaac ggactgagca cgacgctcgg
    13261 acagagcctg gttataagcg tcagaaccga tgcggtcagt gtaacccaga acaactacgg
    13321 aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac
    13381 cttccggttt cagggttgct ttgttgaagg tgaacagaac gtcagacttc agagtgaagt
    13441 gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga gctgcttcgc
    13501 cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga cgagtgccga
    13561 tggtgtgtgc atcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcgg
    13621 gagtgatcgc atactcaaca ccgccagcga agaccggaga aacgccggtg tcgtggtctt
    13681 taaaggatgc gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca
    13741 gacgagtgta gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa
    13801 cgccctgagc tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc
    13861 agtcgtaacc catttcaaag ccaacatacg ggttaacctg gtaaccacca aaagcacctg
    13921 cacccagttg gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact
    13981 gggaccagcc cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta
    14041 cggtagcgaa accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg
    14101 cctcgttatc atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct
    14161 tcaccgggga tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc
    14221 tggcgatgta aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata
    14281 tgaaaaaaaa gtcttgtata aggtacgttt aatctttttt gtcagcgaca atttacagaa
    14341 gaaaatcgcg gaaaccgctt cagacaagcc tccgcaagga aaattagtca cgactgaaag
    14401 cattggctgg gcgataaaaa aagttccagg attaatccta aatttactta atgatacaaa
    14461 ttagagtgaa tttttagccc ggaaagttgt ctcgttgcgt gagaggatgc gcttaccgga
    14521 cgcataataa accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct
    14581 tctgcagtca aatcatctgc caaccaaccg atcaccacgc tgtaattgcc cgtgcgtaaa
    14641 gcgcgaacca ttgactccac ggtgtggcaa ggggagagct ggctaatctg cattactttc
    14701 gttaagggta gcccagatgc ctgaacccat tcgcgactca gtttttgttg cggtgttaac
    14761 cagagttgcc agcgtgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc
    14821 gtcatcatgg gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt
    14881 tccgtagaga cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca
    14941 tagcctgaag tgtacataat caatccagcc cctgtgagtt actgtatgaa tgtacagtac
    15001 atccagtgac gacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg
    15061 gtggcgggaa aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt
    15121 aacggatccg ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa
    15181 aagcctctcc tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat
    15241 tgaataccga tcattgtttg gtagttacag cctgaccgtt gacgacacgg tgtttgcgat
    15301 ggtttctgat ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa
    15361 acatccgcct gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta
    15421 tcgagttgat gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc
    15481 tctcgatgca gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt
    15541 gcccaatatg tcttttcatc tggaagcgat tctcggggag gtggggatta aggatgtacg
    15601 ggcgttacgt atacttgggg caaaaatgtg ttggttgcga ctgcggcagc aaaactgtct
    15661 ggtgacagaa aagattctgt ttatgcttga aggggccatt atcggcattc atgaagctgc
    15721 gctcccagtg gcacgccgcc aggagcttgc agaatgggct gactctctta cgccgaaaca
    15781 ggagtttcct gcggaacttg agtaatctgg cgttgcagac gaccaatctc tggcagtaat
    15841 gcaatcaata acccaacttg ttgtacgacc agaggttctt tgctgtctgc ccgtggttca
    15901 agttgctgca tccgctgttt caggccagct aatgcctgat tgacgcgttc ttcatcagca
    15961 ggttgatgat gtagcgcgtc atcaacatag caaacggcgt catcaagaaa cgccagaatt
    16021 tcaggattag ttaactgctc ccggtgagca ccgagggctg agatatagct ggtaaacgta
    16081 tggttaaggc acagcaaccg aaacgcggct tcgcgaattt gcggggtaac gttcggttcg
    16141 ctggacatat ttgataccac cgacgccagt tcagcatcac ggttgtgtgc atcgcggcgg
    16201 gcaatacgat acgccagacg gttatcacgc ccctgatggt attgttccag tatggcatcg
    16261 agataccggc agttggcctc tgtggcgcgt tcgagcattc gcggcagatt gcgaaacttc
    16321 cagtcaggcc agatgtagct cactgccgcc cacgcaatgg cacaaccaat cagcgtatcg
    16381 attacgcgag gtaacgccac ttcaaaacct tcacccagca agttgaaaca cagtagcacc
    16441 aaaagtgtga tgaacatcgt tgcatgggcg tattgcacgt tacggaaggc aaaaaagagc
    16501 acgccggtaa taaccagcag caccagctgc ccttccaatg atggcacaaa ccacagcaca
    16561 ggaataccaa ttgcgatacc taccagcgta ccaataatcc ttaacttcag gcggtggcgc
    16621 gtggcgttat agtttggctg gcagacaaac aaacttgtca gcaagatcca atacccgtga
    16681 tgcattccgg ttatctgaat gatggcgtag ccgaagcaca acaccagcga cattcttacc
    16741 gcatgacgga agagggcgga ttccggcgtg aagtgacggc taagacgcag ccagatatca
    16801 ctcaatccgt gcgggctgtc atcagcgagc tcattttcgt cattattatg gggcagtgcc
    16861 tgggcctgtt ctgattcaat tgttgccagt tgggcatcaa tggcacgtaa attgttcagt
    16921 aaaaatccca gtgttttgag taaatcagca ggcgcgccgt tatcgcgcat ccgctccagc
    16981 gcagcatcaa tatgcgtaaa agcgcgctca aaatgcggat catgttgata aggctgacgc
    17041 agcaaaatac agcgtgacag ttgctggcac gcctggccct gcatcgacat cagccgctga
    17101 aaacggaaca gcacgtcgct gtggcgaaaa tgttcacgca atgtttgata ttgaatatga
    17161 gaagagctgg cacgctcgtg aatatcctgc gcgacaaagt aataatgcag tgtgcgacgc
    17221 gttccccgtt gaccacgatc gccacgtaag cgggtcagca gcgagagttt cgtctgattc
    17281 aatgtcgcca tcagctgacc gttggcgaga gccaaatcgt acagcggtgc ctggctttca
    17341 tcttcaatat caggatcaaa catgcgcgac ttgagctcaa gataacgcgc cagttgttca
    17401 tagcaacgcg ccaggttgtc ctgcagcggg cggaccggga acagcagatg accaataagt
    17461 gtcaggacgt tgtaccagac ggcaccggcc agaagataca tcggctgctg ataccagtgc
    17521 tcatacagtg atgttcccaa catagtgtaa atggcgatca gcaatgcacc gaaggcaatt
    17581 gttgcatagc gttgacccag accgccgagc aaaatgaagc cgctggtaga gagcgttaag
    17641 ccaatcgcaa atagccaggg ccagggaaac agcaattcta ctgaggccga ggcgataaaa
    17701 aagcagaaca gcgtaatgat gaggttacgc aaacgtcccg ccagtcggtc atcgagatcg
    17761 gtcagcgccg ctgccaccat cccaagcgtc agcggaatcg tcagttttac atcacccagc
    17821 caccacggaa acgctgtggt tccacaaagc gcaataaaaa tacgcgcgta atacagccag
    17881 gcgctgttcc aggtatagcg tttgagcaaa ggacttagca taaaggccat attagtggat
    17941 tattcaaaac gacgacgtgc attggcttcg cgcgcagctt gcgctgtttc taccgatacc
    18001 acgcgacgac cgaccggcca tagtgcaata gcggcaattt taaagttcgc aataccgaca
    18061 ggaatgccaa tgatggaaat acattgtgcg atgcccgttg caatgtgcat caggcataac
    18121 caccagccaa agaaaatcag ccagaaaata ttcaataccg taccgccagt attcagcagc
    18181 acatttttgc cagccgggtt cagttcatcg acatgaatag cttcattgcc ataaggcacc
    18241 agagacagtt tagtgatctc ccagcaggat cgtgtcagtg gtaaggtaaa aatcagcaca
    18301 atactgacca gagtcgccaa cagccagccc agagtggtgg caaatccgcc aagcacaaag
    18361 ttcagaatgt tcaaaacggt acgcataaaa cctcgcttta ctgtggtttt cagtaatggg
    18421 cggcaattgt aacgtttttt tgggctggag cacgttttct ctgacggtta cactgataag
    18481 aaataatttc gtgtggatct acagagtcat ggaactgaaa gcgacaacgc ttggaaaacg
    18541 tctggcacag cacccttacg atcgggcggt gatcctcaat gccgggatta aagtctccgg
    18601 cgatcgccac gaatacctta ttcctttcaa tcaattactg gcgattcact gtaagcgcgg
    18661 tctggtatgg ggcgaactgg agtttgtact gccggacgaa aaagtggtgc gtctgcacgg
    18721 caccgaatgg ggcgagacgc agcgttttta ccatcatctt gatgctcact ggcggcggtg
    18781 gagtggcgag atgagcgaaa ttgcgtctgg cgttttacgt cagcaactgg atttgattgc
    18841 cacgcgcacc ggagaaaata aatggctgac gcgtgagcaa acctctggta tgcagcaaca
    18901 aatccgccag gctttgtcgg cgttaccatt gccggttaac cgactggaag aattcgataa
    18961 ctgccgtgag gcgtggcgta aatgtcaggc ctggttgaaa gacattgaag gcgctcggtt
    19021 gcagcataat caggcgtata ccgaagccat gcttaccgag tatgcggatt ttttccgcca
    19081 ggtcgagtct tcaccgctga atccggcgca ggcccgggca gtcgttaatg gcgagcattc
    19141 tttgttagtg ctggctggtg caggaagcgg aaaaacgtcg gtgctggtgg cccgtgcagg
    19201 ctggttgctg gcgcgtggtg aagcgtcccc tgagcaaatt ttattgctgg cgtttggtcg
    19261 caaagccgct gaagagatgg acgagcgtat tcgagaacga ctacataccg aagacattac
    19321 cgcacgcaca tttcatgcgc tggcgctgca tattattcag cagggcagca aaaaagttcc
    19381 gatagtcagc aaactggaaa atgataccgc tgcccgtcat gaacttttta ttgctgagtg
    19441 gcgcaagcaa tgcagcgaaa agaaagcgca ggcgaagggc tggcggcaat ggctgacgga
    19501 agaaatgcag tggtcagtgc cagaaggtaa tttctgggat gatgaaaaat tacagcgtcg
    19561 tcttgcctct cgcctcgatc gctgggtaag tctgatgcgg atgcacggtg gtgcacaggc
    19621 agaaatgatt gccagtgcac ccgaagagat tcgcgatctg ttcagtaaac gtatcaagtt
    19681 gatggccccg ttattaaaag cctggaaagg tgcgctgaag gcagaaaacg ctgtcgattt
    19741 ttcgggcctt attcaccagg cgattgtgat tctggagaaa ggtcgcttta tcagcccgtg
    19801 gaagcatatt ctggttgatg aatttcagga tatctcgccg cagcgtgcag cgttgttagc
    19861 ggcattacgc aagcaaaata gtcaaacgac gttgttcgct gttggtgatg actggcaggc
    19921 gatttaccga ttcagcgggg cgcaaatgtc gctcaccacg gctttccatg aaaactttgg
    19981 tgaaggtgat cgctgtgatt tagacacgac ctaccgtttt aacagtcgta tcggtgaggt
    20041 ggcaaacagg tttattcagc agaacccagg ccagcttaaa aagccgctaa acagcttaac
    20101 caatggagac aaaaaagccg tcacattatt ggatgagggg caacttgacg ctttgctgga
    20161 taagctctct ggttatgcca aaccggaaga gcgcattctg atcctggcgc gttaccatca
    20221 catgaggcct gccagcctgg aaaaagcggc aacacgctgg ccgaagttgc aaatcgactt
    20281 tatgaccatt catgccagca aagggcaaca ggcggattac gtcatcatcg ttggcttgca
    20341 ggaggggagt gatggttttc cggctgcggc gcgggagtcg attatggaag aggcgctact
    20401 gccaccggtt gaggatttcc cggacgctga agaacggcgg ttaatgtacg tggcgctgac
    20461 ccgggcacgc catcgggtat gggcactgtt taacaaagag aatccctctc cttttgtcga
    20521 aatactgaaa aatctggatg tgccggtggc gagaaaaccg taagaaacag gtggcgtttg
    20581 ccacctgtgc aatattactt cagacggtcc gcgagataac gctgataatc ggggatcaga
    20641 atatcgaccg cgtcgttaaa atgcggtgac tggataatga agtctgccgt tgccacgttg
    20701 gtggcgaccg gaatgttcca taccgttgcc agacgcagca aggctttcac gtcaggatcg
    20761 tgcggcacgg catttagtgg gtcccagaag aaaatcaata catcaatttt cccttctgag
    20821 atcaatgcgc caacctgctg gtcacccccc attgggccac tcaacatcgc gttgacgttc
    20881 atgccggtcg cgcgggaaat taagttaccg gtagtgcctg ttgcatacag tacgtgttgt
    20941 tccagtaacg gttgatgccg ttccacccag ctcatcagca tttgtttgca gtgatcgtgt
    21001 gccaccagcg caatatgttt ccgcgaaggt aaagtgcgag tcgtcagttc cataatgtac
    21061 atccgtagtt aactttccta cagattactg taagtactta tcgctgcaag ataaagactg
    21121 aaaaagcctg cgcacaggca caaaaatctc aggaggatgg ttgtttttcc gcccattgca
    21181 ggaaagtatt tcgcgtttgt gggtcagcca gtttaaacca atacttcagc cgttgttctg
    21241 tgagcacctg agactgcggt ggaatagcat ccagctcgga aacacccgaa agcagcgtac
    21301 tgtcatctgc catcatcgtg gcaaattgcg gtagtgaagc gcgtttcgcg gatttgttat
    21361 agcgttcaac ctcgacctca taatcaatcg tttttgccgt tgaggtaatg gcgagaatat
    21421 ccagctttac cggaatcggc gtcgcatcgc catccaacaa ttcaaggcgc ggcgcggcat
    21481 caaaatggtt agcttcccgc tcattctcca ggcgaggcag gcgaaaattg acctggttga
    21541 tgagctgggt attaaaacta accaccagtg gaggggagat atagagccgt tcttcactat
    21601 tggaaagatg aatcgtcttc tcaacgcgaa acactaactg atgcggtcca ttgtccagtt
    21661 caatgctgtc cgcaccgcgc agcagagagc tggaaacctt cttaccgtcg agtaccagaa
    21721 gatcgacatc agtagaaagc cgcagcgtgg tggcaaaaac ggataccggc aaacataatg
    21781 caatcaaggt ggtcacgatg ccggttttca tagatag
//
LOCUS       DAPQZD010000072        21437 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPQZD010000072 DAPQZD010000000
VERSION     DAPQZD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.072"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            18..872
                     /locus_tag="RO454_002650"
     CDS             18..872
                     /locus_tag="RO454_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDY0934465.1"
                     /translation="MFTLVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLES
                     IFNNTQVQIAVLIIPTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSDRTVRIQ
                     VGYGLEEKVTDALAGDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQYSVNPSE
                     SESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDK
                     TFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSGGGGSSG
                     GGGASGRW"
     gene            complement(932..2230)
                     /gene="eno"
                     /locus_tag="RO454_002651"
     CDS             complement(932..2230)
                     /gene="eno"
                     /locus_tag="RO454_002651"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDY0934466.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2318..3955)
                     /gene="pyrG"
                     /locus_tag="RO454_002652"
     CDS             complement(2318..3955)
                     /gene="pyrG"
                     /locus_tag="RO454_002652"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDY0934467.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4183..4974)
                     /gene="mazG"
                     /locus_tag="RO454_002653"
     CDS             complement(4183..4974)
                     /gene="mazG"
                     /locus_tag="RO454_002653"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDY0934468.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5070..7304)
                     /locus_tag="RO454_002654"
     CDS             complement(5070..7304)
                     /locus_tag="RO454_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDY0934469.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7352..8653)
                     /gene="rlmD"
                     /locus_tag="RO454_002655"
     CDS             complement(7352..8653)
                     /gene="rlmD"
                     /locus_tag="RO454_002655"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDY0934470.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8710..11466
                     /gene="barA"
                     /locus_tag="RO454_002656"
     CDS             8710..11466
                     /gene="barA"
                     /locus_tag="RO454_002656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDY0934471.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11697..13037)
                     /gene="gudD"
                     /locus_tag="RO454_002657"
     CDS             complement(11697..13037)
                     /gene="gudD"
                     /locus_tag="RO454_002657"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDY0934472.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13058..14398)
                     /locus_tag="RO454_002658"
     CDS             complement(13058..14398)
                     /locus_tag="RO454_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDY0934473.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14400..15752)
                     /gene="gudP"
                     /locus_tag="RO454_002659"
     CDS             complement(14400..15752)
                     /gene="gudP"
                     /locus_tag="RO454_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDY0934474.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16186..16635)
                     /locus_tag="RO454_002660"
     CDS             complement(16186..16635)
                     /locus_tag="RO454_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDY0934475.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16653..17435)
                     /gene="truC"
                     /locus_tag="RO454_002661"
     CDS             complement(16653..17435)
                     /gene="truC"
                     /locus_tag="RO454_002661"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDY0934476.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17435..17764)
                     /locus_tag="RO454_002662"
     CDS             complement(17435..17764)
                     /locus_tag="RO454_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDY0934477.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18386..18931)
                     /gene="syd"
                     /locus_tag="RO454_002663"
     CDS             complement(18386..18931)
                     /gene="syd"
                     /locus_tag="RO454_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDY0934478.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18999..19847
                     /gene="queF"
                     /locus_tag="RO454_002664"
     CDS             18999..19847
                     /gene="queF"
                     /locus_tag="RO454_002664"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDY0934479.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQLAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19959..21323
                     /gene="ppnN"
                     /locus_tag="RO454_002665"
                     /gene_synonym="ygdH"
     CDS             19959..21323
                     /gene="ppnN"
                     /locus_tag="RO454_002665"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDY0934480.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5249 a         5729 c         5124 g         5335 t
ORIGIN      
        1 tgcgctattt catactaata ttcacactcg tgtgttcctt tgttgcagcc caaccaacta
       61 ttgtcccaca attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa
      121 agaaagaact gacgcgcaag ctagaatcta ttttcaataa tacccaagta caaattgctg
      181 tattaattat tcccacaacc aaagacgaga caattgaaca atatgctgca agggtttttg
      241 acaattggcg tttaggagat gccaaacgca atgatgggat actgatcatt gttgcctggt
      301 cggatcgcac tgtccgcatc caggtaggtt atggactgga agaaaaggta accgatgctc
      361 tggctggaga tatcatccgt agcaacatga tacccgcatt taaacaacaa caattagcta
      421 aaggattaga gttagctata atcgctttga ataaccgact cacttcacaa caccaatatt
      481 cggttaatcc ttcagaaagt gaatcagcat cttccagcga tcattactac tttgctattt
      541 tttgggtatt tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt
      601 gtcgcgcatg taaaagcagt gtctgtattt cagcaatcta tcttttagat ttattcctgt
      661 tctccgataa aactttttcc attgctgtat ttttcttctt ttttactttc accaccatta
      721 tggtctttac ctgtttatgt gtacttcaga aaaaagcatc tggtagaagt tatcattcag
      781 acaacagcgg ttccggtggc ggctcaagtg gcggcggatt tagcggcggg ggcggttctt
      841 ctggcggtgg cggcgcgtcc ggtcgttggt aactcaacca taaaaaacgc cagcccgaag
      901 gctggcattt ttaaatcaga taaagtcaga cttatgcctg gcctttgatc tctttacgac
      961 cgttgtacgg tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag
     1021 caacacggtc agaacggctc atagaacccg ttttgatctg gcctgcagca gtaccaacag
     1081 ccaggtcagc gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt
     1141 agccagcgtc tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt
     1201 tgaatttgat caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg
     1261 tgttggttac gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggttt
     1321 ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc ttcgatggaa acgatcggat
     1381 actgtttggt cagttcttcc aggaagtgag tgaattcttc agaggtgaat gctttgttgc
     1441 cttcgccagc cagaacgtat ttaccgtctt tgtagaattc agaagctgcg cagtccatcg
     1501 ccagagtgat gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag
     1561 ccagagcttc agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaacagcag
     1621 tgttcatgcc tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc
     1681 ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa
     1741 cgttgttgtc agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt
     1801 atttgcccgg agtgccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag
     1861 ctgcagcagc tttggcgtta gccagagata cagccaggat tgcgtttgcg ccgaatttgg
     1921 atttgttttc agtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag
     1981 catctttgcc aatcagcgcc tgagcgatcg ggccgttaac cgcagcaaca gctttggtta
     2041 cgcctttgcc caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac
     2101 cagtagaagc acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt
     2161 cggcttcaac agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga
     2221 ttttggacat taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc
     2281 gtacccaggg tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga
     2341 actcgctggc ggcttttaca aagccagcaa acagcgggtg accatcacgt ggagtagaag
     2401 taaactccgg atggaactgg caagccacga accacggatg attcggaact tcgatgatct
     2461 cgaccaactg atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt
     2521 tcaacagcat gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat
     2581 tgtacagctg gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca
     2641 tggtaccgcc gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc
     2701 actcggtaat cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg
     2761 cgttctccat gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc
     2821 aaatgcccag ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt
     2881 ctacgccacg atagccgaaa ccgccaggta cgaggattgc gtccagacct ttgaggattt
     2941 caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat
     3001 tcttcagccc accgtgtttc agtgcttcga tcacggattt gtatgcatcc ggcagctcaa
     3061 tgtatttgcc gaccatacca atggtgactt cacttaccgg attcgcttct tcgaagataa
     3121 cctgttccca ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat
     3181 aatcgtccag cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt
     3241 tcagagaaat aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt
     3301 tcgctggaac ggcgcgatct gaacgacaaa tcaggatatc aggctggata ccgattgaaa
     3361 gcagctcttt tacagagtgc tgagtcggtt tggttttgac ttcacctgac gctgccatgt
     3421 acggcaccag cgtcaggtgc ataaacaggg tgtgctcacg gccaatttca acagccatct
     3481 ggcgaatcgc ttcgaggaac ggcagggatt cgatatcacc gacagtaccg ccgatttcga
     3541 ccagtactac gtcatgacct tcgccgcctt ccagcacgcg ctctttgata gcgttagtga
     3601 tgtgcggaat aacctgtacg gttgcgccga gatagtcacc gcggcgttct ttacgcagaa
     3661 cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga
     3721 aacgctcgta gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt
     3781 ccccgtgttg gattgggctc atagtaccgg gatcgacgtt gatgtacgga tccagtttca
     3841 tgatggtcac attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc
     3901 ctttacccag agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga
     3961 acctgagaag ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg
     4021 aacatgacct gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat
     4081 caggagtgag aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc
     4141 atttaaaaaa tgacacaaat cgcgcttgac cacgtaattc ccttagagat caatttcctg
     4201 ccgttttacc tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag
     4261 tccacgcgcg gcaacaatac gctccacttc gcgaaaacga cgctcgaatt tttcgttcgc
     4321 tttttgcaat gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa
     4381 cagcaggtca cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcctc
     4441 gtacatcacc tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca
     4501 gtcgaaacca acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa
     4561 actacgagga atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt
     4621 gatttgctcc caacgggcaa gcacttcact actattttcg gcagaactgt cagcaaaaac
     4681 atgcggatgg cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa
     4741 gcgcccttct tcctgagcta tttgtgcgta aaacaccacc tggaacagca gatcgcccag
     4801 ttcaccgcgc agatcgtcaa aatcttcacg ggcgatggcg tccagcacct cgtaggtttc
     4861 ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt
     4921 ttccgggtcg cgcaggcgct gcatgatggt gagcaaacgg tcgatttgat tcattgaatt
     4981 atcctgaaaa ttgcgggtcc gtcaggtgta ataccttcga gtaaaagata tcgctggata
     5041 cggctgacgc catatccagc tatgtaagat taactcccgt gcaaccgacg cgcgtcgata
     5101 acatccggta cctggttgag tttacccagc acgcgaccca gcacttgcag gttgtaaatc
     5161 tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca
     5221 agtacgttca ccttctcgtt ggcgagaatg gtggtgatat cacgtaacaa cccactacga
     5281 tcattagcca ccacgcggac caccagcgaa tatccggcgg agtagctctc gccccatacc
     5341 gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg ccaattgttc gcaatcggcg
     5401 cggtgtactg aaataccgcg cccctgggta atgaagccga caatctcatc tccaggaatc
     5461 ggctggcagc agcgcgcgat gtggtgcatc aggttaccga ccccttctac caccacacga
     5521 ccgttatctt tactgcggtt ttgcggcgtg tagctttttt gctgaagctg tttcagcgcg
     5581 gcggcgtcct gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc
     5641 tgattgagac ggatatcccc gccaccaatc gccgccagca actcgtcgac atcattgaag
     5701 ttgtaacgcg gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc
     5761 tcgtcgtcaa ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac
     5821 caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac
     5881 cagtcacggc tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc
     5941 agctggtagg tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg
     6001 acatcactgt ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg
     6061 acatcacctt tcggcgtaaa gacgtacact cggtcgtcaa agacctgact acgtacttcg
     6121 tcgagcattt cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag
     6181 gcaatccggt cttcatgtcc cgaacgtgcg ccgccagcag ccgcgccctc tttatatttc
     6241 cagtgcgcag caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc
     6301 tcaacggttt tgccacccgg ccccagaacc acggtatgaa tagactgata accgtttggt
     6361 ttcgggttag cgacgtaatc gtcaaactca tccggcaggt ggcgatagtg agtgtgcact
     6421 atccccagtg cggcataaca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca
     6481 tcaaacagct catcgaaggc gaggttcttt ttctgcattt tgcgccagat gctgtagatg
     6541 tgtttcggac ggccatacac ttcggcttta acgccttcag ctttcatctc agcgcgcaga
     6601 tggccgacaa actcttcgat atagtgttcg cggtcgagac ggcgttcatg caacagtttt
     6661 gcgatgcgtt tgtattcggt cgggtggaga taacggaagc agtaatcttc cagctcccat
     6721 ttcagttgcc caatcccaag acggttcgcc aacggcgcgt agatattggt gcactctttt
     6781 gccgccagta cacgttcatc ttccggcgca tcttttactt cgcgcagatg agcaatacgc
     6841 tccgccagtt tgatgactac gcagcgaaaa tcatcgacca tcgccaataa catccggcga
     6901 acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc
     6961 gccgccatat cacgcacgcc gtgaataagg ttaacgaccg acttaccgac gctctcgcgc
     7021 agcacatctt cgctgactac gttggcatca gccagaggga atagcagcgc cgcccgcagc
     7081 gtgtcaatgt ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat
     7141 aacagactgg catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct
     7201 aagcactcac acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca
     7261 aattcaccag ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg
     7321 gaccagacct gccgaaatcg gcaaatcgta actatttaac gcgcgagaaa agtaccatcg
     7381 attccagatg tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc
     7441 ctgcttttaa taacgcttcg ctatcccgag ccagcgttgc agggttacag gatacataaa
     7501 ctatacgaat aggttccagt tttataattt gctgcataac acctgcggca cctgctcgcg
     7561 ccgggtccaa caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt
     7621 cttcaagatt ttcgtgataa aacgtcacat tctgtaagcc attaagacgc gcattctgct
     7681 ggcctttttc caccagcgcc ggaacacctt ctacaccgac cacactggca gcttgtgtcg
     7741 ccaatggcag tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt
     7801 ccacatccag ccattccaac gcacgcgcta ccattttttg gttcacaccc gcattgacct
     7861 gaataaaatc gcgcgggcta aaagttaagc gcaacccatt tgagtcatac cagggcatct
     7921 caccagagac ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag
     7981 aatgcgaaaa gcattccagt ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca
     8041 aaatcattag cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgcatag
     8101 cttgtaagct gcccaggcat gccctgactt tgggcagcaa tgcttctagt tggggcacta
     8161 aaatggggca ttgtttaacg tcgacaatgt cactggagcc cgctttgcga aaccccatct
     8221 gaagttgctg tgttttcggt aagtagttca gacttaaacg cgcgcgacgg cgatagcccc
     8281 aaggaacatc ggcgatcact tcagagactt cgtgtttcat taatcgggcg agtgccgcac
     8341 ttttgcttcg ctgctgtaaa tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc
     8401 caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt
     8461 tagcgcgggc atactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgcggca
     8521 ataatccggg gataaataat gttttgccgt tatgtcgcgc cacaccctga ccaaaagagt
     8581 cgaggtcgtt gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt
     8641 agaattgcgc cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa
     8701 cggaactcca tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg
     8761 accgtcctta ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg
     8821 cagcgtcaac tggaagatgc cggtgccagc attattgagc cgcttgcagt ttctactgaa
     8881 tatggcatga gtctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt
     8941 cgccattctg atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc
     9001 acctccaatt ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct
     9061 cgccagctca ctgtcactcg tgacggcgat attatgatcc tccgcacgcc gattatttct
     9121 gagagttact cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgttg
     9181 ggatatattg cgcttgagct ggatcttaaa tcggttcgct tgcagcaata taaagagatc
     9241 tttatttcca gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc
     9301 ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt
     9361 cgcgggcaac tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa
     9421 aacggtatca actcgatggc aatgtcgctg gcggcttatc acgaagagat gcaacacaat
     9481 atcgaccagg cgacgtccga tctgcgtgag acgctggagc aaatggagat tcagaacgtt
     9541 gagttggatc tggcgaaaaa gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg
     9601 gcaaatatgt cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg
     9661 acgctgaaaa cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg
     9721 gcaaataatt tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagccggt
     9781 aagctgattc tggaaagtat tccgttccca ctgcgcagca cgctggatga agtcgttacc
     9841 cttctggcac actcttccca cgataaaggg ctggaattga cgctcaatat taagagcgac
     9901 gtccccgata acgtgattgg cgacccgcta cgtttacagc aaatcatcac taacctggtg
     9961 gggaacgcga ttaaattcac cgagaatggc aacatcgata ttctggtgga aaaacgtgcg
    10021 ctgagtaata ccaaagtgca aattgaagtg cagattcggg ataccggcat tggtattcct
    10081 gaacgcgatc aatcgcgctt attccaggcc ttcagacagg ctgatgccag tatttcccgc
    10141 cgtcatggtg gcaccgggct ggggctggtg attacacaaa aactggttaa tgaaatgggc
    10201 ggtgatattt cgttccatag cctgccgaat cgtggttcaa ctttctggtt ccacattaat
    10261 ctcgatctga acccgaacat tattatcgaa gggccatcca cccagtgcct cgctggtaag
    10321 cgcctggcct atgtcgaacc aaactccgca gcagcacaat gcacgctgga tattttaagt
    10381 gaaacgccgc tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac
    10441 gacatgatgt tgttaggcat cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag
    10501 cgattagcga aagcggtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca
    10561 caagtcaatg ctgaaaaact taagcaagat ggtatcggcg cgtgtctgct gaaaccatta
    10621 acacctacgc gcctgttgcc tgccctgacg gaattttatc atcacaaaca aaacacgctt
    10681 ttgcctgtaa ccgatgaaag taagctggca atgacagtca tggcggttga tgacaacccc
    10741 gctaacctga aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc
    10801 gatagcgggc atcaggcagt tgaacgggcg aaacagatgc cgttcgattt aatcttaatg
    10861 gatattcaaa tgccggacat ggacggcatt cgggcatgtg agctcatcca ccagctcccg
    10921 catcagcagc aaacgccggt tatcgcggta acggcgcatg cgatggccgg gcaaaaagag
    10981 aaactgcttg gcgcggggat gagtgattat ctcgccaaac cgatcgaaga agagcgattg
    11041 cataatttgc tgttgcgcta caagcctggc agcggtattt cctctcgcgt cgtgacgccc
    11101 gaagtcaacg aaattgtggt caacccgaat gcgaccctcg actggcaact ggcactacgc
    11161 caggcagcag gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg
    11221 cctgaagttc gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt
    11281 gatttgattc ataaactgca tggcagttgc ggctatagcg gtgtaccgcg tatgaagaat
    11341 ctctgccaac tgatcgaaca acagctacgt agcggtacta aagaagaaga tttggagccg
    11401 gagctgctgg aactgttgga cgagatggat aatgtagcgc gcgaagccag caaaattctc
    11461 gggtaatgga cgtcttgtcg agtagcaacc ggatttttat gacgctggtt tcagattggt
    11521 gacaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc ttatccggcc tgcaaaacca
    11581 attaaattca atgaattaaa aaatgatgta gccctgatac gtagcgcaac aggcaatttt
    11641 gcgtttggca tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa
    11701 cgcaccatgc acggacgctt gttatcgaac gtccagccgg ggatcaggta ctgcattccc
    11761 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcgtgcgc tttcatcact
    11821 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgaac cagaccgcct
    11881 ttgatctcta acggttcttt ggtcaggcgc tgattacctt cctgccagat ccagtgcgta
    11941 tcaatcgcgg tgattttacc cggtgcggca gcggcaacat gggtaaacat cgccagggaa
    12001 atatcgaagt ggttgttgga gtgtgaaccc caggtcaggc caaattcatg acacatttgc
    12061 gccacacgta ccgaaccttg catcgtccag aaatgcggat ccgcaagcgg gatatcaacg
    12121 gattgcaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgcg
    12181 gtcggcaggc cagttgcgcg acggaactct gccatcactt cacgcccgga gaaaccttgc
    12241 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtatgt accgatttta
    12301 atcgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaaa
    12361 cgttgcgcca gtgccacaat agattctgct tcttcttccc cggccagtac accgcccttc
    12421 agtttgaaat cgttgaagcc atatttttca tatgccgctt ccgccaggcg caccaccgca
    12481 tccggcgtca tcgcttcttc gtgacgcagg cgataccagt cacattgatc atccggctgg
    12541 ctttgatacg gcagcggtgt ggctttgcga ttaccgacga agaacagata accgagcatt
    12601 tcgacttcgc tacgctgttg gccatcgccc agcagcgatg ccacgtttac ccccagatgc
    12661 tgccccaaca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    12721 aaatcaaatg tctgcaaacc gcgcccaccg gcatcacgat cggcaaacgt attacgcacc
    12781 agcgtcagaa cgtttttgta ttcacccagc gttttaccca ccaccagcgg aatcgcatct
    12841 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa
    12901 ttatctttga taatcacaat attacgcgtg aagaacggtg catgtgcacc actcagattc
    12961 atcagcatac tgtcatgacc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    13021 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacggggc
    13081 gtttacggtc aaaggtccag ccggggatca ggtactgcat cgggcctgcg tcgttacgcg
    13141 caccgccagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    13201 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    13261 ttttggtcag acgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    13321 tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatca aagtggttat
    13381 tagaatggca gccccaggtc agcccccagt cgtcgcaaag ttgcgccacg cggactgcac
    13441 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    13501 catgacccat ttcgcgccag ttggtggcaa tcatgttggt cgcgacgggc aagccggtcg
    13561 cccgtcgaaa ctccgccatc acttcacgac cggagaaacc ctgctcagcg ccgcacgggt
    13621 cttccgcata ggtaagaaca tcattcagcc ctttgcacag agaaatggct tcatcaagca
    13681 gccaggcacc attgggatca acggtaatcc gcgcatccgg gaagcgtttc ttcaatgcac
    13741 gaacagtgtc gatttcttgc tcgccaggta acacgccacc cttaagtttg aaatctttaa
    13801 agccatagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    13861 tctgatgccg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    13921 cggttttggt ccgatcaccg atataaaaca gataaccgag aacggtaata gcatcacgtt
    13981 gcttgcctgg ccctaacagt tcgcagaccg gaacattcag cgcctgacct aacaggtcaa
    14041 gcaaggcggc ttccagcgcc gctacggcat taacgcgcaa ttcaaaagtc caggcacctt
    14101 tgccgaaggt atcaaaatcg gctgcctgat tacctttatg cacctgctga accactttat
    14161 tcaggcgcgc aacttcctgg cccagcacca tcggaatagc cccgaccagt gtttgataaa
    14221 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcattatcg gtgagtacca
    14281 caatattgcg ggtgaaatat gcgttatgtg caccaccaat attaagcaac atgctgtcat
    14341 gtccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgtcatct
    14401 tattgccccg cgacaggttt cagctcaata cgcttgatat cgcccaccag caccaggtag
    14461 ctgagtaccg cgattaaggc atgaacacca acataaatca gcgccccatt aaaggagcca
    14521 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccaaac
    14581 atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcagtatc agccattaca
    14641 gcccaaccca gcgcaccgat ccccttaccg aagaaggcca gcgccataaa gccgatgatc
    14701 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacaacaa catgcccatc
    14761 acgatcggtg ttttacgcgc aatgttcagt gaacccgtgc gacgcatcag ccagtcggaa
    14821 ataatccccc ccaacacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    14881 cccgctttca gaatcgacat cccgcgcgcc tgcaccagat aaaccgggaa ccaggtaatg
    14941 aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    15001 gacccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    15061 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tgtactccag ctctttctga
    15121 tttacgcccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    15181 atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg agtcagccag
    15241 cccataatcg gcgcgaagat aaccgttgcg aagtattggg cggagttaaa aatcgacacc
    15301 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcggctgtt gccagggaaa
    15361 gatggcgctt cagcaagccc gaccaggaag cgcagtgtaa acagggccac gataatgccg
    15421 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    15481 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accagccacc agggatctgc
    15541 ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    15601 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagcgcggtc gccgtagttg
    15661 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    15721 acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    15781 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgtttttgc
    15841 cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat agcagtataa
    15901 aaagcgcgcc atagcggctc accgtgcaac aacacaacat taatgcgttc aatgaggccc
    15961 gattttggca ttagccctgg acggtggaat ccacttcacg gaaatgaaaa caagaacaag
    16021 aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa atggatcgct tgactccagg
    16081 caaacgccag taaaaatccg cgctatgaag cagtttttac tggcatttgc ctgaaaagat
    16141 tcgattcagc accgctaaaa cggcatttac cgctcgctga acatatcagg acaacatcgt
    16201 gccccaatgt tcaacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    16261 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    16321 tttgccgcca ttgcagaaat tcacataact actgtcgccg agcgcaatca cgccataacg
    16381 cagattcggc tggaagccca gggtatcttt aattccctga aagagcggca caatgctatc
    16441 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    16501 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gcgcggtcag
    16561 aatcgcttcc gcttcttcgg ccactaacag cgaatttccg tacatagtgc cgacaaaaat
    16621 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    16681 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccccgcca gccaaattgt
    16741 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagccagcgg ctcgccagta
    16801 aaaggatgcg tcaatgacaa ctgactggca tgcagcatta accgctgaag gccaaaatgc
    16861 tcagcaccgc tgcgattctg gcgtaaatca ccatgtttgc tgtcgccaat aatcgggtgc
    16921 cgcaaatggg caagatggcg acgaagctgg tgcttgcgtc cagttttcgg ctccagttcc
    16981 accaggccgt agcgcgtggt cgggtaacgt ccagtcgcta ccggcatttc tacggtcgcc
    17041 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    17101 gcgattttgt ccagttcttc caccagtgga taatccagca ccgcttcttc catcaaccag
    17161 ccacgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    17221 agccgtccgg cttcgctgga taatcccatc aacaacacac cagaagttgg tcggtccaga
    17281 cgatgagcag taaaaacatg ctggcctatc tggtcacgca cggtttgcat gaccactact
    17341 ttctcgtcgc gatccagcca gctgcggtga accagccagc cggagggttt atttaccgca
    17401 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    17461 catccagttt ctccagctca gccagaataa gcgcgcgttg cggatgatca gtcgccagcg
    17521 ccatttcata atagggcgca acagcaaaag cgcccggtaa cggctggttg ttatctaaca
    17581 aatcgtgcat tcgcgggatc agcacccact gcaaccactc cagtggttcc atggtgtcca
    17641 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    17701 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    17761 tcataaagca acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga
    17821 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc
    17881 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    17941 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    18001 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    18061 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    18121 ccttcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    18181 tagaatcgtc tttttctcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    18241 ttcatatgaa agttaagaat taacaagcaa catgttgttt ttaaatgaga ataacgattc
    18301 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    18361 ctcacaatca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    18421 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac
    18481 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    18541 tgggcggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    18601 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    18661 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aatcgtgggt tgtatcacaa
    18721 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    18781 agtatacggc atcttcggta gttgaaataa tgcacggtga aggaacgccg tacagttcct
    18841 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    18901 ctttcagggc tcgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    18961 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    19021 aggcacttgc gggcctgaca cttggaaaat caaccgatta ccgggatacc tatgacgcca
    19081 gcctactgca aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    19141 acctgccttt tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga
    19201 aaggtttgcc gcaactcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    19261 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    19321 gggatgaggt gcgccagacg ctagagcgcg acttaagcac ttgcgcccag ggtaaggtta
    19381 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    19441 cctgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    19501 agaatgccac cagcggtgaa aaagtagtgg aagagacgct tgtcagccac ctgctgaaat
    19561 caaactgcct gatcacccat caaccagatt ggggttccat ccaaattcag tatcgtggac
    19621 gccaaattga cagagaaaaa ctgctgcgtt acctggtctc tttccgtcat cacaacgagt
    19681 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    19741 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    19801 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    19861 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    19921 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    19981 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    20041 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    20101 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgac
    20161 gtgaacgcgg cgtaaagctg gaactgataa atcccccgga agaggctttt gtcgatgggc
    20221 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgtgacatt ctcttcgttt
    20281 atgggcaaat tcataacacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    20341 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtc ggtgaagcgc
    20401 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    20461 gtcgcgtcgg gaatcagttg ggcctgcgtg aactgaatat ctgcaccggc tgtggtccgg
    20521 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    20581 acagtcgctt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    20641 tggtcaacga attgatcatc atgccagata tcgaaaaacg tctggaagcg tttgtccgta
    20701 tcgctcacgg tatcattatc ttccctggcg gtgtgggtac agcagaagag ttgctgtatt
    20761 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    20821 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgtg catacgctgg
    20881 gtgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    20941 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg cgacactggt gacgcttaca
    21001 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    21061 agaatatggc taatctgaag ctttacccgg atcaacccgt tgaagtgctg gctgccgacc
    21121 tgcgccgtgc gttctccggt attgtggcgg gtaatgtaaa agaagtcggt attcgcgcca
    21181 ttgaagagtt tggtccttac aaaatcaacg gcgacaaaga gattatgcgt cgtatggacg
    21241 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    21301 cttgctacga aatctgcacg taatctcccc tcccccggtg acgtttgttg ccgggtttta
    21361 tcgtttttgg tcacttactc atcaactcat ttcatttgtt atatgaatgt ttcttaccaa
    21421 cctcacgcga caaatat
//
LOCUS       DAPQZD010000073        21277 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPQZD010000073 DAPQZD010000000
VERSION     DAPQZD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.073"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..319
                     /locus_tag="RO454_002666"
     CDS             <1..319
                     /locus_tag="RO454_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0934481.1"
                     /translation="LEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLT
                     NTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVS
                     LAG"
     gene            complement(362..1513)
                     /locus_tag="RO454_002667"
     CDS             complement(362..1513)
                     /locus_tag="RO454_002667"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDY0934482.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1527..3122)
                     /locus_tag="RO454_002668"
     CDS             complement(1527..3122)
                     /locus_tag="RO454_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDY0934483.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3269..4027)
                     /gene="aroD"
                     /locus_tag="RO454_002669"
     CDS             complement(3269..4027)
                     /gene="aroD"
                     /locus_tag="RO454_002669"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDY0934484.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4058..4924)
                     /gene="ydiB"
                     /locus_tag="RO454_002670"
     CDS             complement(4058..4924)
                     /gene="ydiB"
                     /locus_tag="RO454_002670"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDY0934485.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMEPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4936..6201)
                     /locus_tag="RO454_002671"
     CDS             complement(4936..6201)
                     /locus_tag="RO454_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934486.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6428..7642)
                     /locus_tag="RO454_002672"
     CDS             complement(6428..7642)
                     /locus_tag="RO454_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934487.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7742..8098)
                     /locus_tag="RO454_002673"
     CDS             complement(7742..8098)
                     /locus_tag="RO454_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDY0934488.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8234..8341)
                     /gene="rprA"
                     /locus_tag="RO454_002674"
     ncRNA           complement(8234..8341)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RO454_002674"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8527..9639)
                     /gene="ydiK"
                     /locus_tag="RO454_002675"
     CDS             complement(8527..9639)
                     /gene="ydiK"
                     /locus_tag="RO454_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDY0934489.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9853..12909
                     /locus_tag="RO454_002676"
     CDS             9853..12909
                     /locus_tag="RO454_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDY0934490.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12906..13316
                     /gene="menI"
                     /locus_tag="RO454_002677"
     CDS             12906..13316
                     /gene="menI"
                     /locus_tag="RO454_002677"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDY0934491.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSVREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13416..13604
                     /locus_tag="RO454_002678"
     CDS             13416..13604
                     /locus_tag="RO454_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDY0934492.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14153..14521
                     /gene="sufA"
                     /locus_tag="RO454_002679"
     CDS             14153..14521
                     /gene="sufA"
                     /locus_tag="RO454_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDY0934493.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14530..16017
                     /gene="sufB"
                     /locus_tag="RO454_002680"
     CDS             14530..16017
                     /gene="sufB"
                     /locus_tag="RO454_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDY0934494.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTVISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16027..16773
                     /gene="sufC"
                     /locus_tag="RO454_002681"
     CDS             16027..16773
                     /gene="sufC"
                     /locus_tag="RO454_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDY0934495.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16748..18019
                     /gene="sufD"
                     /locus_tag="RO454_002682"
     CDS             16748..18019
                     /gene="sufD"
                     /locus_tag="RO454_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDY0934496.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPTKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVMARIGQRLPGGAR"
     gene            18016..19236
                     /gene="sufS"
                     /locus_tag="RO454_002683"
     CDS             18016..19236
                     /gene="sufS"
                     /locus_tag="RO454_002683"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDY0934497.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGVLYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALAQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19249..19665
                     /gene="sufE"
                     /locus_tag="RO454_002684"
     CDS             19249..19665
                     /gene="sufE"
                     /locus_tag="RO454_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDY0934498.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19813..20817
                     /gene="ldtE"
                     /locus_tag="RO454_002685"
     CDS             19813..20817
                     /gene="ldtE"
                     /locus_tag="RO454_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDY0934499.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20880..21116)
                     /gene="lpp"
                     /locus_tag="RO454_002686"
     CDS             complement(20880..21116)
                     /gene="lpp"
                     /locus_tag="RO454_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDY0934500.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5527 a         5152 c         5392 g         5206 t
ORIGIN      
        1 tttagagcag ttatcgaaaa aatttcgtgc cagcgttagt tatatctgcc atgaatttac
       61 caaagagtat cgtatttccc ctattaacta tgttattcaa cggcgtatga cggaagcgaa
      121 atggtcactc actaatactg aattatcaca ggcagagatc tcctggcgtg tgggttatga
      181 aaatgtcgat cactttgcca aactgttttt gcgccatgtc ggctgttcgc ccagcgatta
      241 ccgcaggcaa tttaaaaact gttttgcgga acaagaaatc ctatctgaat ttcctcaacc
      301 agtaagtctt gccggataaa tctggcggcg cagtttgtta agaagctgcg ccgcctgcag
      361 attatttgtt ctgataatct ttcaggatct gccgacctgc tacgtaaatc ataatttcgt
      421 ctgtaccgcc gccgatacgt tcacaacgga catcacgcca gaagcgggag acgcgcgcct
      481 catcggtata gcccagaccg cccatgattt gaatcgcatc atcaatgact tccattgcgg
      541 tacgtgcgca gtacagtttt gccagcgccg cgctggtgcg cagtgactga tgctgatcgg
      601 cttgccatgc cactttcaac accatgttgc gcatgttgtc gatcttaatc gccatcagcg
      661 ccagtttttc ctggatcatc tggttatgac caatgggctt accaaaagcg atacgctggt
      721 tggcatagcg ggcggcatct tcgaatgcgc attcggcaaa gccggtgctg cgcgcggcgt
      781 tgatcaggcg ctccatctca aagttgtaca tcacattgag gaaacccatt ccttcttcgc
      841 ccaccatgtc gctctcttca acttccacgt tgtcgagata gacttcgcag gtgctgagca
      901 tatgccagcc gattttatgc agtggattga ttttaatgcc gggcttactg gagtcgaccc
      961 accacagagt aaaggccttt ttcggatctt tcggttgcgg atcgcgcgcc aacaccagca
     1021 tatatggata ctctttcgca ccggtaataa aggttttctg tccgttgatg taaaccttgc
     1081 cgtttttacg cgtgtaagtg gtagtggcac tgttgttgtc tgagcctgcg cctggctccg
     1141 tcaatgccag ggcataggcg ggatcacccg tttccagggt gctctctgcc gttttacgta
     1201 gctgctctgc agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg gtaatcaaaa
     1261 atgccggagc accgcatttt gacacttcca tcagcgccag catttgggtg acgtaatccg
     1321 cagggatacc accaaattct tccggcacgc caagcatgga aataccgtta tccgccagtg
     1381 cccgcataaa ctcacgcggg tatgtcccgt tttgatcgca ggtgcggaaa tactcttccg
     1441 gaaagttagt cgtaatcagt tcgcgaatac tggccagcag cagttcttgt tcttcagtta
     1501 aagagaaatc catcattata ctcctgttaa tgagccgctt caggcaggac aaaacccatc
     1561 gccgcatcga taaataatct ttcgtccatc agtttgagtt ctggcgaaat cactggggtg
     1621 aaatccattt tgtcgagaat atctttttgt aaatcgacgc caggagcgat ttcgattaaa
     1681 tgcaggccgt cttcttttag cgtgaatacg gcgcgctcag tgatataacg aacatccagc
     1741 cctcgctcga gagcgatttt tccgctgaaa gttatttccg gtagttcccg aataaatttc
     1801 ttcacccgtc cttcctggac gatatttaat ttgccgtcgg taatttctgt ttttaaactg
     1861 cccgcagtta atgtgccgca gaaaacgatt ttcttcgaag tggcactgat atcaataaat
     1921 ccaccggtgc ccatgatttt accattgaat ttatgcacgc cgacgttacc gtgctggtcg
     1981 acttcagcaa aactcaaata acaaacatcc agaccaccac cgtgataaaa atcaaactgg
     2041 gacgtcatat ccagaatggc gcgggtattc acgttcgcgc caaaggcgat cccctgtgaa
     2101 gtaataccgc caatcggacc tgtttctacc gtcagaataa agtcatcagc acaaccttct
     2161 tcccgggcga ccaggccaat gccgtcagca ataccgacgc cgacattccc cactgcgcct
     2221 ttgcgcattt cgaataatgc acgccgtgca actaatttac gttggtttag gggcaggcta
     2281 agtttggtgc tgtcatcaag ggtgaagtca ccagaaataa agcggttaac cggcgcaccg
     2341 ccatacagtt gggtttgatc cggatcgacc accacaatat ccaccagata acccggaata
     2401 cggacagatt taggatgcag cgtggctttc ttaaccattt tctgcacctg catcatcaca
     2461 ataccgccgt tattgtgcac cgcctgggca ataaccaatg cgtcgagata catcacctca
     2521 tcttcaaaag tggcgtagcc ttcgctgtcg caggtggtag cgcgaatgaa ggcgatatct
     2581 ggcgcaatcg ctttgtaata gagatattct ttgttatcaa actcgaccag tttaatcagg
     2641 tcttctttag tgacttcatt cagtttgccg ccttgctggc gtggatcgac aaatgttccg
     2701 atgccaatat cactaataat accaggctgg tgggctgcgg cggcgcgtaa ggtttgtgta
     2761 agtacacctt gtggatagtt ataagcaata attttatttt gttctgcgag atcagaaata
     2821 cgcggtgatt gtccccagtg accgcataat gcccatttca ccaggccttc ttgcgccagc
     2881 ggactaatac cacggtcggc gcgatcgcca agccctgttg gactaataat cgataaatta
     2941 cgtggtgttt gagtctgttt atatttatca gcaagagcag taattaacgt ggtggcttcc
     3001 agaataccgc cgccagcgcc taacacacaa agtgttgctt cgtcgggaat ataattcacc
     3061 gcttcctgtg ccgataggac cggcactcgt ccattaatac gaggtggttt tacaggtttc
     3121 attgttaatt cctgattcag gtgtgatttt ccctacgcca gatggagaat tagcgcacag
     3181 agactcacga aatattttta ttaaatacgg ttaaaaatac tttttttagt tcggcgggag
     3241 agggtattcc cgccgaaaat attattgctt atgcctgatg taaaatagtt aataccgtac
     3301 gcaaatcatt taccgagatt tgccctggcg cagaggcttt ttttaccgca ccaaaagttg
     3361 ccgccgaccc aaatacttca ccagccagac gagaaattac gccagttttt gccatcgaca
     3421 tcgtgatgat tggacgatcg gcatactgct cctgcatctc cagggtcgcg gcaagcaacg
     3481 tcagcacatc gctggtactt tgcggcatca gcgcaatctt aggaatatcg gcgtcgaagg
     3541 actgcatttt gcgcagacgg gcaatgattt cttcggcttc cggcgtttta tggaagtcat
     3601 ggttggacat gacaactttc acatcatgcg cgtgggcgta ggcgacggtt tctttaacct
     3661 gatcatcgcc ggtaaataac tccagatcga tcatatcaac caggccgctg tcgatggctg
     3721 cacgattgag tgcaatataa gcctcggtgg aaatcgcctg ctcgccgcct tctttggcac
     3781 tgcggaaggt aaacagcagc ggtttttctg gcatggtttc acggagaatt tttgctgccg
     3841 ccatgacaga ctccacattg gagaggtcgg catagtggtc cacacgccat tccagaatat
     3901 caaagtccgc ttcacgatag gcgagagctt cggatttcac gctggcgata tctttcgcca
     3961 tcagcgagac gatgatttta ggtgcgcccg taccaatgac gagatctttt acagttacgg
     4021 ttttcatttt ttaccctttc tgcacgcggt cagcctgtca ggcaccgaac cccatgacct
     4081 gtttaacata ttccagaggg aaatctttgc cagtccataa cgtgaactgt tcagcccctt
     4141 gccacaacaa catgccgtat ccatcaatcg ttttgcaacc cgcttgttgc gcctgctgca
     4201 ataacttcgt catatgaggg ttatacacgc attcagtgac cagaagtccc gggtgtaaca
     4261 gactgatatc attaaccaat gattcattct caaggggttc catacccact ttggtgccat
     4321 tggttaaaat gtcggcggaa gccagggctt ctgcaaaggc ttgctgatcg gcgagatcgg
     4381 tgactgtcac gacgcaatcg gtgttttcgt taacccgctg cgcgaaggcg agagctttat
     4441 cgaagaactc atcccgacgg ttaaagagtt taatttcttt taaaccttca attgccccct
     4501 gcgcgccaat tgccgttgag gcaccaccgg cccctaatag caccatcgtt ttgcctttga
     4561 tatcaaaacc gctctcttta atggcgcgaa tatgacccgt gccgtcggtg ttatagccac
     4621 gcagatagcc atcatcattt acgatggtgt tgatggcacc caccagtttg gccgctggcg
     4681 ttaattcatc aacatattca cacgccagtt gtttgttcgg cattgataca ccagttccgc
     4741 gcattttgag ggcttttaat ccttcaattg ctccgggaaa actatcgtta tccacttcaa
     4801 aggccatata ggtaaatggc aatcccgctt tttctaaggc tttattctgc atttcgggcg
     4861 ataaactgtg gcggatggga taggccatca acccaatcaa ttcgtatttt gcggtaacat
     4921 ccataattaa ctcctttaac ctctatgctt aattgtgttt aaccgggtgc taaatttatg
     4981 ctcgccaaac cgcacatcat tttcaggaat aatgaaaacg cggtaatagc ggataaaaac
     5041 aataattgcg gtaatcaggg ccagcagcgc gaaagtaaaa tcgagaacaa tgatatattg
     5101 caggccgatg ttcgacagat aaccggtaat cagcggaata acaaagttag ccagtccacc
     5161 catcatcata tatatactgg tgactttggc tttgcttttt gggaaaaact ctgacatgac
     5221 cgaaacgccg agctgtaaaa tgccgccagc tgctgagaaa ccgataacaa aggctccggc
     5281 attacacacc aatggagatg ggtacaggta gataatggca gctgttattg ttgccagtgc
     5341 agagttaaat acattagccc agatgggccg gaccattttt ttcagcagtg cggcaaaaat
     5401 aaagacacag accagcgaac ccatactgta ataagagatg gtttttaatg cctcagcttc
     5461 tgacatacca gcaaaagcca tcgcatattt gggcatccac accacaatca cataaaaggt
     5521 cgagaatgcg gctacaccga acagtactga tgaaacacct tccagccaga ctaacggttt
     5581 gctgttcatt tgcggtaatt cattagctac gctggcgtcc accaactggc tggggaattt
     5641 gcttttcaac agcatcagcg tgatcagtac aaacagaata cccggaataa tcagcccata
     5701 gccgtaccag atgttattga gcaacatata gctcaccagc attggataga acatttgccc
     5761 gaatgacacc atcgctttaa ccagtatgac cgccgaacca gaggctttcg gaaagcattc
     5821 catgagcgcg gggtagccac ccgtatccag cgctgagtta gcgataccta cgcacactgc
     5881 cagacagtag gcgagagtta aattcgggca agcaggaata ccaaagaaga atagcagata
     5941 cattattact gccattaata tcaccgcccg acgaccaaac ttatcggaga tcactccgaa
     6001 gaataaaata ctgatcaatc gccccaatcc gataccggaa attaaatagg caatgcccgc
     6061 gttgtcagtg gaaaactttt ctgccaaaga tgtcatgttt tgggcaagcg taataacact
     6121 aatgccgtgc aggaaatagc tgaagtaaat acaaagaaca gccaggataa atggcgtgct
     6181 gaaagcctta ttttgagaca tgttcacagc tccttgctga aagataattt cgttaccgta
     6241 cgtattgttc tccgacggtt tacataatta ctatactgaa gatgattttc cctgggtaca
     6301 tggcataaag tttatgtttc caggaatatt gcatgtcata gtttgttttt gatcgctatt
     6361 ttccttagtt agcgcagaaa gagttattta tccgtaagat cttgcataac ctgaaaagaa
     6421 tattgcatta cccacccgga gcgatatttt cctttagcgt gtgatgccgc gtttctttgc
     6481 gactgcgtaa gccaataaac agtgccagta taaagccgat agcagcgatg gcggtatcga
     6541 accacataat atcggcaata cttctttgcg aaagatgagc cgtaatcaac ggaatggtaa
     6601 atgttgcaat gctgcccgca ctgtaataga tccccgtagc tttaccttta gcgtagggaa
     6661 aacgttccgc cattaacgtc aggccaattt gtacaacacc gcctgcggag gtaaaaccga
     6721 tgacgaaagc aaatattatc accacataaa atgtgggatg caggcagacg gtgagcaggg
     6781 cgataaatga gataaaggtg tacagcatca gtaatgttgt tgggcgaacg gtattacgaa
     6841 tgagtggagc agtaataaat acacacagca gcgaacccac ggtgtagata ctgagtaatt
     6901 tgatcgacat ggtgtatgac atgccagcga caaattgtcc gtattgtgcc agccactggc
     6961 taaccagata aaacgttgcc attgagatat agccatatag ggaataactg gctaaatcga
     7021 taattgaaca gcgatgttcc gtagagctgg tggttttctt tatgacaggt aagtgacgac
     7081 ccggatgggg tgggaacgta caacggtata aaaacagagc gttaataaac ataatgcctg
     7141 cggcaatcat aaaggaccaa ccgaaccaca gttcagccca cactaacagg ctgataatta
     7201 gcggtaataa aaattgtccg ctggaaacaa atgctttaat taaaatattg gctgtacccg
     7261 gtgagcgtgg aaaagcttcc atcaaactgg gataggtgcc tgcatcgaga aaactgtttg
     7321 ccattcccgc cagaaagcca aaaacataag cgataatgat gttattggtg tgcaggatgc
     7381 caaaaaagaa ggccatatag cagcacatcc cgagcatgat aaaagggcgg cgaccaaagc
     7441 gatcggataa taatcctgca aaaagcaaga cacttaatcg accaatgccc agtgatgaga
     7501 taactatcga gacgcccgcg gcattagtct gccaaagcgt ctccagcgag gccatattca
     7561 ggctcatcaa aatgacgccc ataccatgca ccaggtaatt aaaatacaac ccaagtgcgg
     7621 tagggtaata gggatttttc ataggttcta cttagcaaag gtgctttaaa aagtgtagcg
     7681 atgttgtgtc agtatccgtt cattattgtt gtaacaacga caattccttt ctgtggagaa
     7741 attaacaaag ccgctccaga tcgtgtgcat ataattcagc ggctaccgat tgatagatct
     7801 tccagtcaat aaaattgcca tcagggtaga cttgttgaaa cgcggttaag tcggggaaaa
     7861 aacgcatggt gttgttgccg atgcggttat ttattttctc tatgattgca tgaagatgtt
     7921 tttttcgttg ctgacgcata gccaacagtt gctcgactac ataatcagga atggcgcatt
     7981 tgccattttc ccactgacgc cagctttcac tgttaccggt ctgggcaatc caggtcgcgc
     8041 attcatcaat agtcatggca aaaatatggc gaagtgcctg gagttcataa gcgttcatcg
     8101 tgtaatggct gcatgaaata aacaataaaa tataaataac attatcagcc tgctgacggc
     8161 ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg cgaggtagcg
     8221 aagcagaaaa atgttaaaaa aaagcccatc gtgggagatg ggcaaagact acacacagca
     8281 attcgttgtt tcactcaggg gatttccatg cttataaatc aatatgttga tttataaccg
     8341 tgagctaata gtaggcatac ggactttttg cgtcgatcag attcgtctca ttagtttaaa
     8401 aactgtaaag aatttgcgac aggtactacc agctgaatag cggtaagcag gaaaatatgg
     8461 ggaagagcaa gcaaatatca attagcaggg gttaacaatc aggcggcaga gccgcctgat
     8521 gagaaattac ttattcgatt tctcgatttc gccaagttct tcgagaattt cttccggttg
     8581 gtccgtcggc ggtgggactt cttccaccca cgcggcaaac agacgccagg aaacggctaa
     8641 cagaaccgga ccaataaaca gaccgatcat cccgaaagca atcaaaccac caataacgcc
     8701 agagaggatc aggatcagcg gtaaatcggc acccatgcga attaacattg ggcggatgac
     8761 gttatcgagc gtgccaacca caccgctcca aactaacaat accgttcccc aggtggtatc
     8821 gccagtccag tagagccaga taatcgccgg aatcagtacc ggcaacggtc caagctggac
     8881 aaggcaggag aggatcatta acaccgttag caaagttgca taaggtacgc cggatacggc
     8941 gaggccgata ccgccaagca ctgcctgtac taacgccgtc accaccacac ccagcgccac
     9001 cgcgcggata gcctgtgccg ccagcagcac ggcggcatct ccgcgaacgc ctgccagacg
     9061 ggttgcaaaa tggcgaatgc cttgtgccac ctgttcaccg cgccagtaca gcagggcgct
     9121 gaagagaagc atcagcgcac aatgcaccat aaagcgcccg atatgcgccg cctgcccaac
     9181 gaaccaggtg gtggtggtgc caatataagg acggactttc gccatgatcg ccgtcccccc
     9241 catatccagc aaattgtgcc agcctgcata tagcttcgcg ccaatcaccg gaatggtatt
     9301 aagccacgcc agatcgggta acgtcatgtc accactggaa atggctttaa ttagcgggcc
     9361 gctgccgtcg acgatactgt taaccagcaa agcaataggg atgataaaca ccatcactaa
     9421 taacagcgtc atcaccagaa cggcgagaga gcggcgacca aacatgatct tttgcaaacg
     9481 taacaatacc ggccaggtgg cgataaccac tgtaccggcc catgcaaagc cgagaataaa
     9541 gggttgtaca atccacagac atgccacaat catgatggct aaaaacagca ccgaaagcag
     9601 aatttgtgcg acatccctgg gctgacgaac atttaccata actacttttc accttttttg
     9661 tgcgtcaaat cgtcgacgcg acgtgaacac gcgaaactca cctgcataat gatgagcaat
     9721 ttcagcggtt tttaacaggt cgattctgcc cttaattctg atgggcgtgt tagaaaaaaa
     9781 tgtggtacaa caactcaggc aacacgcaaa cgattaatct ctcatcacag accgcaggaa
     9841 agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca actggtgctt
     9901 aattttttgc aagagctgga gcaacagggt tttaccggcg atacggcgac aagttatgcc
     9961 gatcgtctga caatgtcgac tgacaacagt atttaccaac ttctccccga tgcggtggta
    10021 tttccgcgtt caaccgcaga tgtagcgctg atcgcccgtc ttgccgcgca ggaacgctat
    10081 tcatcgttga tctttacccc ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac
    10141 caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat taaccctgaa
    10201 gagggctggg tgcgcgttga ggccggggtg attaaagacc aactcaatca gtacctgaaa
    10261 ccctttggct acttttttgc gccagaactt tcgaccagca accgggcaac gctcggcggg
    10321 atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac gtcagatcac
    10381 gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca acctttaccc
    10441 gtcgaactgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat ttataacacg
    10501 gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc caagcttaac
    10561 cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac cgagttcgac
    10621 ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga agcgcgactg
    10681 gatattacgc gcttgcctaa agtacgccgt ctggtgaacg tcaaatatga ctcttttgac
    10741 tccgcgctgc gtaacgcgcc gtttatggtt gaggcgcgcg cgctttcggt agagacggtg
    10801 gactcaaaag tgctcaacct tgcacgggaa gatattgtct ggcattccgt cagcgagttg
    10861 attaccgatg tgcctgataa agagatgctt gggctgaaca ttgtggaatt tgccggtgat
    10921 gatgaggcac tgattgacga gcgagttaat gcactctgtg tgcggcttga tgagctgatt
    10981 gccagccaac aggcaggagt gatcggctgg caggtgtgcc gcgagctggc gggtgttgaa
    11041 cgtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa aggtgctgct
    11101 aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacacctggc ggattatatt
    11161 gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt cggtcacgtc
    11221 gacgcaggtg tcttgcacgt ccgtccggcg ctggatatgt gcgatccgca acaagagatt
    11281 ttgatgaagc aaatctctga tgatgtagtg gcgcttaccg cgaaatacgg tggtttgttg
    11341 tggggcgaac acggcaaagg ttttcgcgcc gaatacagcc cggcgttttt cggtgaggaa
    11401 ctttttgcag aactgcgtaa agtgaaagcg gcatttgacc cgcataaccg actcaacccc
    11461 gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag
    11521 cgcggtacat tcgatcgaca gatccccatt gcggtacgcc agcagtggcg tggtgcaatg
    11581 gagtgtaacg gcaacggttt atgcttcaac tttgatgctc gtagtccgat gtgtccgtcg
    11641 atgaagatca cccagaaccg gattcattca ccgaaagggc gcgctacgct ggtacgtgaa
    11701 tggctgcgtt tgttggcgga tcgcggcgtt gacccactca aactggaaca agaacttcct
    11761 gaatccggag tcagtttgcg gacgttaatt gcccgcacgc gcaatagttg gcatgcgaat
    11821 aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg tctggcctgt
    11881 aaagcgtgtt cgactcagtg ccccatcaaa attgatgtgc cggagtttcg ctctcgtttc
    11941 ctgcaactct atcacacccg ttatttacgc ccgctgcgcg accacctcgt cgctacggtt
    12001 gagagttacg caccgctgat ggcacgcgcg ccgaaaacct ttaatttctt cattaaccag
    12061 ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc gctgctgtcg
    12121 gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac gctggaacag
    12181 cttgaagctc tcaatgcaga gcagaaagcg cgcacggtgc ttgtggtgca ggaccccttt
    12241 accagctatt acgatgctca agtggtggcg gattttgtcc gtctggtcga aaaattaggt
    12301 ttccagcccg tgttactgcc attttcgcca aatggcaaag cccagcatat caaaggtttt
    12361 cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag
    12421 ctgggtatgc caatggtagg cgtcgacccg gcgctggtac tttgttatcg cgatgaatat
    12481 aaactggctc tgggcgagga gcgtggcgag tttaacgtct tactggcgaa tgaatggctg
    12541 gcaagcgcac ttgagtcaca gccagtggct acagtcagcg gtgaatcatg gtatttcttt
    12601 ggtcactgta ccgaagttac cgccttgccg ggcgcgccag cacaatgggc ggcaatattt
    12661 gcccgttttg gcgcgaaact ggaaaatgtc agcgtggggt gctgcggcat ggcagggact
    12721 tacggacatg aagcgaaaaa tcataaaaat tcgctcggga tctatgagtt atcctggcat
    12781 caggcgatgc agcgactgcc gcgtaatcgc tgtctggcga ccggatattc ctgccgtagt
    12841 caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt actggagatt
    12901 attaaatgat atggaaacga aaaatcaccc tggaagcact gaatgctatg ggggaaggaa
    12961 acatggtggg gttgctggat attcgctttg aacatattgg tgatgacacc cttgaagcga
    13021 caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga ggtgcatccg
    13081 tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgag ggtgagcaaa
    13141 aagtggttgg tctggaaatc aatgctaacc acgtccgctc agtacgagaa gggcgggtgc
    13201 gcggcgtatg taaaccgttg catctcggtt cgcgtcacca ggtctggcag attgaaatct
    13261 tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgctatt ttgtgacgtt
    13321 gtcaccagaa aagtgtgacg catattgctt ttggttatta tctgcacagt aaacggtggt
    13381 atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt gatcccaccc
    13441 aactggccat tgaattttta cgccgtgacc aaagcaatct ttcacctgcc caatatctca
    13501 agcgtctgaa acaattagaa cttgaatttg ctgacctgct tactctctct tctgctgaat
    13561 taaaagaaga aatctatttc gcctggcgtc tgggcgtgca ttgatcctga tttaatgtcg
    13621 cgagttcgcc aaatcggcct ggattaatct tcgtccaggt tcgagggcct tcttaatact
    13681 gcctatctac ccgcctgata acgttcaaaa ataatacaaa tcgcagtttg tgttaaaaca
    13741 gtgggttagc tttatgagat tatccttatc gccccttcaa gagctaagcc actgtgagtg
    13801 ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg
    13861 ttttcttatt tctgaacgct gcactttttt acctatccct taaatagtca ttttcaatgc
    13921 aactttttct ttttcatcac agccttaatg ctccttagat aataatcatt atctaacaat
    13981 gagataccta attcttagcg tgcctgttaa cccacaaatc agggtctatg cttattaaaa
    14041 gcaggtaaaa agcatgtttt acgctaaagc ccctgcgttt gctgggttga actgataatc
    14101 attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat cgatggacat
    14161 gcattcagga acctttaatc cacaggattt cgcctggcaa ggcttaacgc tgacacccgc
    14221 agcggcgata cacatccgcg aactggtggc aaagcagccg ggtatgatcg gcgtgcgctt
    14281 aggcgtgaag caaacgggct gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc
    14341 ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc
    14401 gatgcctttt attgatggca cggaagtcga tttcgttcgt gaaggactta atcagatatt
    14461 caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct ttggggtata
    14521 ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg gaccggcggc
    14581 ccgctgaatt ataaagaagg attcttcacc cagttagcca ccgatgagct ggcaaagggg
    14641 ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga gtggatgctg
    14701 gagtttcgtc tcaacgccta tcacgcatgg ctggagatgg aagaaccgca ctggttgaaa
    14761 gcgcactacg acaagctgaa ttatcaggat tacagctact actcggcacc atcgtgcggt
    14821 aattgtgacg acaactgcgc gtctgaaccc ggcgcggtgc agcaaactgg cgcgaacgcc
    14881 tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc
    14941 aaagaggtgg cggtggatgc cattttcgac tctgtttcgg ttgccactac ttatcgcgaa
    15001 aaactggcgg agcagggaat tattttttgt tcctttggcg aggcgatcca cgatcacccg
    15061 gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt ctttgccgca
    15121 cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg cgtgcgttgc
    15181 ccgatggaac tttccaccta ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc
    15241 accattctgg tggccgacga agacagctac gttagctaca ttgaaggctg ttccgctccg
    15301 gtgcgtgaca gctatcagtt acacgcggcg gtggtggaag tcatcatcca taaaaacgcc
    15361 gaggtgaaat attctacggt acaaaactgg ttccctggcg ataacaacac cggcggtatt
    15421 ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg
    15481 caatcagaaa ccgggtcagc gattacgtgg aaatatccca gctgcatttt gcgcggcgat
    15541 aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcaaca agcggatacc
    15601 ggcaccaaga tgatccacat cggtaaaaac accaaatcga ccgttatctc gaaagggatc
    15661 tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc gacggcaacc
    15721 aatgcgcgca atttcactca gtgcgactca atgctgattg gcgctaattg tggggcgcat
    15781 accttcccgt atgtcgaatg tcgcaataac agcgctcagc tggagcacga ggcaacgaca
    15841 tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag cgaagaagat
    15901 gccatctcga tgattgttaa cggtttttgc aaagacgtgt tctcggaact gccgctggaa
    15961 tttgccgttg aagcacaaaa actcctcgcc atcagccttg agcatagcgt cggataagga
    16021 ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc tatcctgcgt
    16081 ggattaagcc tcgatgttcg tcccggcgaa gttcacgcca ttatggggcc aaacggttcg
    16141 ggcaaaagta ccttatcggc aacgcttgcc gggcgagaag attatgaagt gacgagcggc
    16201 acggttgagt ttaaaggcaa agatttgctt gcgctgtcgc cggaagatcg tgcaggtgaa
    16261 ggcatcttta tggccttcca gtatccggtg gaaattcccg gcgtcagtaa ccagtttttc
    16321 ctgcaaacgg cacttaatgc ggtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt
    16381 gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga agatttatta
    16441 acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga tattttgcaa
    16501 atggcggtgc tggaaccgga gttatgcatt cttgatgagt cagactccgg gctggatatt
    16561 gacgcattaa aagtggttgc cgatggcgtg aactcgctgc gtgacggcaa gcgttcattc
    16621 atcattgtta cgcactatca acgcattctc gactacatca agcctgatta cgtccatgtg
    16681 ctgtatcagg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca actggaggag
    16741 cagggttatg gctggcttac cgaacagcag taacgcactg caacagtggc atcatttgtt
    16801 tgaagctgaa ggggcgaaac gctccccgca agcacagcag catttacaac aattgctgcg
    16861 taccggactg ccgacacgta aacatgaaaa ctggaaatat acgccgctgg acgggctgac
    16921 caatagccag tttgtctgca ttgcggggga gatatcccca cagcagcgtg atgccttagc
    16981 gttaacgtta gacgccgtgc ggctggtgtt tgtcgatgga cgttacgtgc ccgcactgag
    17041 cgatgcaact gaaggcagcg gatatgaagt gagcattaac gacgaccgtc aggggttacc
    17101 cgacgctatt caggcggaag tgtttctgca tttgacggaa agcctggcac aaagcgtgac
    17161 gcatatcgcc gtgaagcgcg gtcaacggcc gacaaagcca ttgctgttaa tgcatatcac
    17221 ccagggcgtg gcaggtgaag aggtgaatac tgcccattac cggcatcatc tggatctggc
    17281 ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg ctcgtcactt
    17341 caccggcgca cggttcacta tcaacgtcgc agcgaacgcc catttgcagc atatcaagct
    17401 ggcgtttgaa aacccgctca gtcaccactt tgctcataac gatttgttgc tggctgatga
    17461 tgccaccgca tttagccaca gtttcctgct gggtggcgca gtgttacgac acaacaccag
    17521 tacgcaactc aatggcgaaa acagcacggt gcggatcaat agcctggcga tgccggtgaa
    17581 aaacgaggtg tgtgatactc gtacctggct ggaacacaat aaaggttttt gtaacagccg
    17641 acagttgcac aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa
    17701 cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc tgctgatggg
    17761 caaactggcg gaagtggata ctaaaccgca gctggaaatc tatgcagatg atgtgaaatg
    17821 cagccacggc gcgacggtgg ggcgtattga tgacgaacag atgttttatc tgcgctcgcg
    17881 cgggatcaat cagcaagatg cccagcagat gatcatctac gcctttgctg ccgaactgac
    17941 ggaagcactg tgtgatgagg ggcttaaaca gcaggtgatg gcccgaatcg gtcaacgact
    18001 gccaggaggt gcaagatgac tttttccgtc gacaaagtgc gggccgactt tccggtgctt
    18061 tctcgtgagg taaacggttt gccgctggct tatctcgaca gtgccgccag tgcgcagaaa
    18121 ccaagccagg tgattgacgc cgaggctgag ttttatcgtc acggctacgc ggcggtgcat
    18181 cgcggtattc ataccttaag cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg
    18241 gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg
    18301 gaagggatca atctggtcgc caatagctgg ggcaacagca atgtgcgagc gggcgataac
    18361 atcatcatca gtcagatgga gcaccacgct aacattgtcc cctggcagat gctttgcgca
    18421 cgcgttggcg cagagctgcg tgtgatcccg ctcaaccccg acggtactct gcaactggag
    18481 acgctgccta cgctgtttga tgagaaaact cgcctgctgg caattactca tgtctccaac
    18541 gtgcttggga cagaaaatcc gctggcggag atgatcacac ttgcgcatca acatggcgca
    18601 aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg
    18661 gattgcgatt tctacgtgtt ttccgggcat aaactgtatg gtcccaccgg cattggcgtg
    18721 ctgtatgtca aagaagcctt gttgcaggag atgccgccgt gggaaggggg cggttctatg
    18781 attgccaccg ttagcctgag tgaaggcact acctggacca aagcgccgtg gcggtttgaa
    18841 gccggtacgc ccaataccgg aggtattatt ggccttggcg cggcgctgga gtatgtttcg
    18901 gcgctggggc ttaataacat agccgagtat gaacagaatc tgatgcacta cgcgctagca
    18961 cagctggaat ctgtaccgga tctcactctc tatggcccac agaacaggct tggcgttatt
    19021 gcttttaatc tcggtaaaca ccacgcctat gatgttggca gttttctcga taattacggc
    19081 attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta caacgtcccc
    19141 gcgatgtgtc gggcgtcgct ggcaatgtac aacacccatg aagaagtgga tcgtctggta
    19201 actggattgc aacgtattca tcgtctactg gggtaaaagg gaggctctat ggctgtattg
    19261 ccggataaag aaaaattgct gcgtaacttt ttacgctgcg ccaactggga agagaaatat
    19321 ctctacatta ttgaactggg acagcgtctg ccagaattac gtgacgaaga caaaagcccg
    19381 caaaatagca tccagggctg ccagagtcag gtgtggattg taatgcgcca gaatgcgcag
    19441 ggaattattg aattgcaggg cgacagcgat gcggccattg taaaagggct tattgcggtc
    19501 gtctttattc tttacgatca aatgacgccg caggatattg tcaatttcga tgtgcgcccg
    19561 tggtttgaaa aaatggcgct cacccaacat ctcaccccat cacgctcaca aggtctggaa
    19621 gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct
    19681 ttcatccggt tggctcacat tgtcgggtgg tcaatgttta cactggcctg cacaggagtg
    19741 ttttggtttc aggtgaacta agcgccatcc gacgtttcag cgtgagtctc cggcaaatac
    19801 aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagc
    19861 gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggcag ccgactggtt
    19921 gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc catcgcccga
    19981 cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca
    20041 aaacccggta cgacgataac tattccttca caactgttat tacctgatgc accgcgccag
    20101 gggataattg tgaaccttgc agaactgcgc ctttattatt atccgccggg agaaaatatc
    20161 gtgcaggttt atccgatagg tattggtttg caggggctgg aaacgccggt gatggaaacg
    20221 cgtgtggggc agaaaatccc taacccaacc tggacgccta cggcgggcat tcgtcaacgt
    20281 tcgctggagc gtggcattaa attaccgccg gtcgttcctg ccgggccaaa taacccgcta
    20341 ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt
    20401 gcgccggaca gcgtcggttt gcgcgtcagt tcgggctgta ttcgcatgaa tgcaccggat
    20461 attaaagctt tattctccag cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg
    20521 gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg accactatcg
    20581 gcagaagaac agcagaacgt tcagacaatg ccgtacacgc tgccagcagg ctttacgcaa
    20641 tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca
    20701 gggtatccgg ttgcggtgag cagtggagca actcccgcag ccagcaatgc gccttcagta
    20761 gagtcagcgc agaatgggga accagagcaa gggaatatgt tacgcgcgac gcagtagcgg
    20821 taaatagcag gcaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat
    20881 tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct
    20941 ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta
    21001 gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct
    21061 gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt
    21121 aataccctct agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt
    21181 atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc
    21241 atcgaacgct gggttttaga taaaaaaatt gagagaa
//
LOCUS       DAPQZD010000074        21014 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPQZD010000074 DAPQZD010000000
VERSION     DAPQZD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.074"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            112..714
                     /locus_tag="RO454_002687"
     CDS             112..714
                     /locus_tag="RO454_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDY0934501.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(753..1178)
                     /gene="nudI"
                     /locus_tag="RO454_002688"
     CDS             complement(753..1178)
                     /gene="nudI"
                     /locus_tag="RO454_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDY0934502.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1457..1999
                     /locus_tag="RO454_002689"
     CDS             1457..1999
                     /locus_tag="RO454_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934503.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            2099..3301
                     /locus_tag="RO454_002690"
     CDS             2099..3301
                     /locus_tag="RO454_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDY0934504.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3521..4303
                     /locus_tag="RO454_002691"
     CDS             3521..4303
                     /locus_tag="RO454_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDY0934505.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4318..5523
                     /gene="rhmD"
                     /locus_tag="RO454_002692"
     CDS             4318..5523
                     /gene="rhmD"
                     /locus_tag="RO454_002692"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDY0934506.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5580..6869
                     /locus_tag="RO454_002693"
     CDS             5580..6869
                     /locus_tag="RO454_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934507.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6887..7690
                     /gene="yfaU"
                     /locus_tag="RO454_002694"
     CDS             6887..7690
                     /gene="yfaU"
                     /locus_tag="RO454_002694"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDY0934508.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(7731..7964)
                     /locus_tag="RO454_002695"
                     /pseudo
     CDS             complement(7731..7964)
                     /locus_tag="RO454_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7965..8960)
                     /locus_tag="RO454_002696"
     CDS             complement(7965..8960)
                     /locus_tag="RO454_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDY0934509.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(9153..10343)
                     /gene="glpC"
                     /locus_tag="RO454_002697"
     CDS             complement(9153..10343)
                     /gene="glpC"
                     /locus_tag="RO454_002697"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDY0934510.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10340..11599)
                     /gene="glpB"
                     /locus_tag="RO454_002698"
     CDS             complement(10340..11599)
                     /gene="glpB"
                     /locus_tag="RO454_002698"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDY0934511.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11589..13217)
                     /gene="glpA"
                     /locus_tag="RO454_002699"
     CDS             complement(11589..13217)
                     /gene="glpA"
                     /locus_tag="RO454_002699"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDY0934512.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13490..14848
                     /gene="glpT"
                     /locus_tag="RO454_002700"
     CDS             13490..14848
                     /gene="glpT"
                     /locus_tag="RO454_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDY0934513.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14853..15929
                     /gene="glpQ"
                     /locus_tag="RO454_002701"
     CDS             14853..15929
                     /gene="glpQ"
                     /locus_tag="RO454_002701"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDY0934514.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            16132..16782
                     /gene="inaA"
                     /locus_tag="RO454_002702"
     CDS             16132..16782
                     /gene="inaA"
                     /locus_tag="RO454_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDY0934515.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16836..17090)
                     /gene="yfaE"
                     /locus_tag="RO454_002703"
     CDS             complement(16836..17090)
                     /gene="yfaE"
                     /locus_tag="RO454_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDY0934516.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17090..18220)
                     /gene="nrdB"
                     /locus_tag="RO454_002704"
     CDS             complement(17090..18220)
                     /gene="nrdB"
                     /locus_tag="RO454_002704"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDY0934517.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18309..20594)
                     /gene="nrdA"
                     /locus_tag="RO454_002705"
     CDS             complement(18309..20594)
                     /gene="nrdA"
                     /locus_tag="RO454_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDY0934518.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4900 a         5160 c         5627 g         5327 t
ORIGIN      
        1 ttgccggcat cataggattt ttaatattac accaactgct aattatcgcc agaaaagtcg
       61 ctcaatttca cggtaattgt ctggttgcgc ttgtctacag gtggagttta cgtgttagct
      121 ttttgccgat cttcgttgaa gtcaaaaaaa tatttcatca ttttactggc gctcgctgca
      181 attgccggac tggggactca tgccgcctgg agtagcaatg gtttgccacg catcgacaat
      241 aaaacactgg ccagactggc acagcagcac ccggttgtcg ttttgtttcg ccatgctgaa
      301 cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac ggtcaaaggt
      361 acccaggatg cccgtgaact gggcaacgct tttagtgctg atatccctga ttttgatctc
      421 tattccagta ataccgtccg gactattcag tcggcgacct ggttttcggc gggtaaaaag
      481 ctgacggtag ataaacgatt tcttcaatgc ggtaatgaga tttatagtgc aattaaagac
      541 ttacaacgca aagcacctga taaaaatatc gttattttta cccataatca ttgcctgaca
      601 tatattgcta aagataagcg tgatgcgaca tttaaacctg attatctgga tggtttagtc
      661 atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaacca ctaaattttc
      721 aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa acgtaacgtt
      781 tttcgggtgg caacattcaa atcataatgc accagatctt caggttttac ccacgcgtag
      841 tcctgaaact cttcgtttat tttcacatct cggttggcag aaacgcagtc aaaaatcagg
      901 taaatcatat aaatctcttc cttgcgacca tctgcatacg tcttggtgcg aatatcatcg
      961 ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc ttcgcgaatt
     1021 tcgcggcgta gtgcctcttc aattcgttcg ccaggctcca cgccgccacc cgaaagcgcc
     1081 cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata agcaccatca
     1141 ttttgaatca aagggcatac aatagtccgt tgtcgcacag gggtattcct tataaataaa
     1201 aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa tctcttttgc
     1261 gatgataact acatttatta ttatttttgt gtgtatcgat aaagtgtgtt tattttttcg
     1321 gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct tttttcgctc
     1381 attgaatcct ctgcgccagg cagtacaatc ccgcagcttt gggaattttg attaattgct
     1441 tgtgggaaag aggataatga aaaaaattgc gctagcaggt ctggccggaa tgctgttggt
     1501 ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat acaccaatat
     1561 tggtgtcggt tttgggactg aatcgacggg cctggcgtta agcggtaact ggacacataa
     1621 cgacgacgac ggtgatgccg cgggcgtggg gctggggttg aatctgcctc tcgggccatt
     1681 aatggcgacc gttggcggaa aaggcgttta caccaacccg aacgatggtg atgaaggtta
     1741 tgcagcggca gttggcggtg gtttgcagtg gcaaattggt gacagcttcc gcttgtttgg
     1801 tgagtattac tactctccgg attcgctctc cagcggaatt aaaagttatg aagaagcgaa
     1861 tgctggcgcg cgttacacca ttatgcgtcc agtcagcatt gaggctggct accgttacct
     1921 gaacctggca ggtaaaaatg gcaatcgtga taacgccatt gctgatggtc cgtatgttgg
     1981 ggtaaatgcc agcttctgac gccttccggc gcggcgttct gtcgcgccat tcagtttctt
     2041 actacgccct gtctcccttt gcgttatagt gattcgataa caaaagcagg agataaccat
     2101 gttaaaagtg gaaatgttat ccaccgggga tgaagtgtta cacgggcaaa tcgttgacac
     2161 taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat ctcgccgcaa
     2221 tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta gtcagcacgc
     2281 cgatgtgttg atcgttaacg gcgggctggg accgaccagt gatgatttaa gcgcactcgc
     2341 cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca aagagatgga
     2401 acgctatttc catgaacggg gacgagtaat ggcaccgagc aaccgtaaac aagcggagct
     2461 gcctgccagt gctgaattta tcaataaccc ggtaggcact gcctgtggtt ttgccataca
     2521 gcttaatcgt tgcctgatgt tctttacccc cggcgtaccg tcagaattta aggtgatggt
     2581 cgagcacgaa atcctgccgc gcctgcgcga gcgtttttct ttaccacagc cgccggtttg
     2641 cctgcgtttg actacctttg gtcgttcgga aagcgatctg gcacaaagcc tggacactct
     2701 acaactgccg ccgggcgtaa caatgggcta tcgctcctca atgccgatta tcgaactgaa
     2761 actcaccgga ccggcaagcg aggaacaggc gatggaaaaa ctgtggctgg acgttaaacg
     2821 tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc agatcagtcg
     2881 cgaattgcag agccgccagt tcagcctgac gctgagcgag caattcaccg gtggtttatt
     2941 ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg ttccttcaca
     3001 ggaggaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcga accattttgc
     3061 tgggttggca ctggctgttt ctggtctgga agatgagcat ctcaactttg cactagccac
     3121 gccagacggc actttcgctt tgcgtgtaca tttcagtgcc acacgctaca gtctggctat
     3181 tcgccaggaa gtgtgcgcaa tgatggcgct taatatgcta cgccgttggt taaacggcca
     3241 ggacatcgcc agcgagcatg gctggattga ggttgttgag tccatgacct tatctgtctg
     3301 attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc aaaatttgtc
     3361 caacctgttg tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt gtgcgtaatt
     3421 tctccagtcc gctggacgag aaacgggatt tgatgcgctt cacaatttta ctccccctga
     3481 acaacgagac tgattccagt atctaagact ggagtccagt atgttggaat caagcaaagt
     3541 tcccgccctg acacgggcta ttgatattct caatttgatt gcccggattg gcccctgtag
     3601 tgctgcgacg atcattgaca cattggggat ccctaaaagc acagcctatt tgctgcttaa
     3661 tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt gtttgtggac
     3721 caggctggtg gagctttccg gtcatgcgtt aagcaagatg gatctccggg aactggctcg
     3781 accgcgtttg acgcaactga tggataccac cgggctgtta tgtcacctgg ggattattga
     3841 taacggcagc gcttattaca ttttgaaagt ggagtcatca gccactatca gcgtgcgttc
     3901 ccatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc tgcttgcctg
     3961 gcaacctgca gctgtacagc aaagtattat cgaagggctg gtctgggaac gggcgacacc
     4021 cacaacgatt actcatccac aacggctcta cgaagaactg gcgcgtattc gccgccaggg
     4081 ctggagctat gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg cgccggtgtt
     4141 taacgccaat aacgagctta ccgctgcgat ttcggtggtc ggtacccgct tacaaatcaa
     4201 tgaagaatac cgtgattatc tggcgggcaa ggccattgcc tgtgccaggg atatttcacg
     4261 tttgttggga tggaaaagtc cctttgactt acaggcctca taacaatgga gaacatcatg
     4321 accctaccaa aaatcaaaca ggttcgcgcc tggtttactg gcggtgcgac agcagaaaaa
     4381 ggcgctggcg gcggtgatta tcacgaccag ggggcgaatc actggattga tgatcatatt
     4441 gccaccccga tgagtaaata ccgcgaatac gagcagtccc gccagtcatt tggcattaac
     4501 gttcttggca cgttgattgt tgaagttgaa gcagaaaacg gccagaccgg attcgctgtt
     4561 tcgacagccg gtgaaatggg ctgttttatt gtcgaaaaac atcttaaccg tttcattgag
     4621 ggtaaatgcg tcagtgatat caaactgatc cacgatcaaa tgctcaatgc caccctgtat
     4681 tactccggct ctggtggcct ggtgatgaat acgatttcct gtgtcgatct ggctctgtgg
     4741 gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg cgctgttcgt
     4801 gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga gatgggcttt
     4861 atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg gatccgcaaa
     4921 gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg gttaatgctc
     4981 gactgctgga tgagtcagga cgtgaattat gccaccaaac tggcccacgc ttgcgcgccc
     5041 tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg ttatcgcgaa
     5101 ctgaaacaca acgcaccagc ggggatgatg gtcaccagcg gtgagcacca cggcacactg
     5161 caatctttcc gtacgctttc agaaaccggt atcgacatta tgcagccgga tgttggctgg
     5221 tgcggtggct taaccacgct ggtggaaatt gccgcaatcg ccaaatccag ggggcaactg
     5281 gtggtgccac acggttcgtc tgtttactcc caccatgcgg tgatcacctt caccaatacg
     5341 ccattcagcg aattcctgat gaccagcccg gattgttcaa cgatgcgtcc gcaatttgat
     5401 ccgattctgc ttaatgagcc agtaccggtg aatggtcgta ttcataaatc agtgcttgat
     5461 aaacccggtt tcggcgtcga actcaatcgt gactgcaatt tgaaacgccc ctacagccac
     5521 taatcactcc gggcgttgct gtgacaacgg taacgcccac atattctaag gagcttttta
     5581 tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg cgcgcgttta attccgttta
     5641 tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tattggtttt gccaaacaga
     5701 cctaccagat tgataccggg ctgagtaatg aagcttatgc gctgggagca ggcattttct
     5761 ttgtggtata cgcgtttctg ggggttccgg cgaatctttt gatgcgcaaa ctgggggcca
     5821 gaacctggat tggtacgaca acactgctgt ggggatttct ttcggctgcc atggcatggg
     5881 ccgatactga agcgaaattt ctgatcgttc gcactctgct tggtgctgcg gaggccgggt
     5941 ttttccctgg tatgatttat ctcacttcgc aatggtttcc gcagcgtaat cgcgccagca
     6001 ttatggggct gttctatatg ggcgcaccgc tggcgttaac actgggatca ccgctttccg
     6061 gcgcgctgct ggagatgcat ggatttatgg ggcatcccgg ctggttctgg atgtttgtga
     6121 ttgaaggatt gctggcagtc ggcgctgggg tattcacatt cttttggctt gatgacacac
     6181 cggggcaggc acgttttctg agtaaacaag aaaaaacgtt gcttatcaat caactggcaa
     6241 gtgaagaaca acagaaagtg acttctcgat tgagcgatgc gctgcgtaat gggcgagtct
     6301 ggcaactggc gattatctac ctgaccattc aagtggcggt ttacggatta attttcttcc
     6361 tgccgaccca ggttgctgca ttgctgggga caaaagtggg ctttacggcg tcggtggtca
     6421 ccgccattcc gtgggttgcg gccttgtttg ggacctggct tattccgcgc tactccgata
     6481 aaaccggcga acggcgtaat gtcgcagcgc tgacattact ggcggcgggc attggtattg
     6541 gtctctccgg gctgctttct ccagtactgg cgatcgtagc actgtgtgtt gcagccatcg
     6601 ggtttattgc cgtgcaaccg gtgttctgga cgatgccgac acaacttctt tccggtacgg
     6661 cgctggctgc ggggattggt tttgtaaacc tgtttggtgc agtgggcggg tttattgccc
     6721 cgatcctgcg cgtgaaagca gaaacgttat tttccagtga tgcggcggga ttactgacgc
     6781 tggcagcggt ggcggtcatc ggttcgctga ttattttcac tctgcgtgta aatcgcactg
     6841 ttgcgcagac cgacgtggca catcattaaa tagtttaagg aacacgatga acgcattatt
     6901 aaccaatccc tttaaagaac gtttacgcaa gggcgaagtg caaattggtc tgtggttaag
     6961 ctcaacgact gcctatatgg cagaaattgc cgccacttcc ggttatgact ggttgctgat
     7021 tgacggggag catgcgccaa acaccattca ggatctttat caccagctac aggcggtagc
     7081 gccctatgcc agccaacccg tgatccgtcc ggtggaaggc agtaagccgc tgattaaaca
     7141 agtcctggat attggcgcgc aaactctact gatcccgatg gtcgatactg ccgatcaggc
     7201 acgtcaggtg gtgtctgcca cgcgctatcc tccctacggt gagcgtggtg ttggggccag
     7261 tgtggcacgg gctgcgcgat ggggacgcat tgagaattac atggcgcaag tcaacgattc
     7321 gctttgtctg ttggtgcagg tggaaagtaa aacggcactg gataacctgg acgaaatcct
     7381 cgatgtcgaa gggattgatg gcgtgtttat tggacctgcg gatctttctg cgtcgttggg
     7441 ctacccggat aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg
     7501 gatccgtgct gcgggtaaag cggctggttt tctggctgtg gctcctgata tggcgcagca
     7561 atgcctggcg tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga
     7621 tgccctggat caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcgtaaaagg
     7681 tagttattga tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta
     7741 ttcgctgcca gatcggcttc gcttagctga gtggccgcga gcacctgatc acgatcaatg
     7801 ccctgttcca gcatgcgcaa agcaatttta atggcttgtt catgcatgcc ttcctgcaaa
     7861 ccttctaatt taccttcttg ccagccttca atcttaccct cctgccagcc aatttgatgt
     7921 ccttcctgcc gtagccgttc agcaatagtc atcaatctct ccttttaatg acagttcgga
     7981 atatcagcat cagtgagttg ggttgccgcc agcacaatct cacgttcaaa gccctgctcc
     8041 agcatgcgca aggcaatttt aatggcttgc tcatgcatgc cttcctgcaa accttctaat
     8101 ttacctttct gcaaaccctc caatttacct tgctgcaaac cttctaattt accttcttgc
     8161 cagccttcaa ttttaccttc ttgccagcca atttgatgtc cttcctgccg tagccgttca
     8221 gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaag
     8281 cgggtgaaac gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg
     8341 ctgtcattag tgaagtctct aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa
     8401 tcgcggtcgc gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg
     8461 atctcatcgt caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc
     8521 cgggcaagtt gcggatcgtc aaactcatcc agccagttga gtgagtacgg gtagggcgag
     8581 gcttcgccat gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc
     8641 aggtgacgct gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct
     8701 gcagagcttt gatgttcaat cacgcagtag atatagccgt caccctcgct ggtttccacg
     8761 gaccacaaaa catccgagta gtaagcgcgt aaactttttt caataaaact ggtgggttcc
     8821 aggcgtaagg tttgcaggtt gcaaagcttg cgcagcggct ctggtagatg tatttccaga
     8881 aaatcccgtg cggtttcggg tgtgaacata aaggttttaa acaccgcatc atgcggggag
     8941 gaggttgttg attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac
     9001 ggttatccca ggcctttgat atctgccagt ttcagccttt gcgagacgtt ttcagaggaa
     9061 ttttcactgc gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt
     9121 aacaacatgc ttaccgggca tcagaaagga gtttaagcca acgcctgtgc cagcagcgta
     9181 atcggatgtt cgcagcgaag acttgtggac atctcaatct gccatttgca ggtttcgcag
     9241 tcggtgacca ccagatctgc gccgctctct tctatctggc ggaacagtgg tgcgccgatg
     9301 gcttgtgaag tggggtagtt ctcttttttg aaaccgtaag ttcccgcaat accgcagcac
     9361 tgggaatcca gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag
     9421 agcgtccagc ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc
     9481 ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt
     9541 tcgatatgat cgcgcaagcc tttgttgtcg acattcagca cttctgggta ttcgtcgcgc
     9601 agggcaaatg tacacgttga ggaggtggca atcaccggaa tgccttttac tcccacagct
     9661 tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaaaccgttg
     9721 gcgatcagcg gtacgccgca gcatttttct ttactgagca gttgtacgcc ggtatccatc
     9781 gcgttgagca ctttaattaa atctttgcct agttgcggat ggttgtagtt aacgaagcag
     9841 ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct gagccgccac gctgcgatac
     9901 cagcgacgga acgtgccgaa ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac
     9961 gccgcatcaa gcagctgacg caccggtttc agcgaggtag cggtgttgac gatgggtgcg
    10021 aacggcgtgg atacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac
    10081 gggcgcgtgg tgtcatattt cgcccgcgcg cgctggataa tatcgccaat cttcacatcg
    10141 gacgggcagg cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac
    10201 agtgcgccat ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccccgga
    10261 taacccggat tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag
    10321 ttttcgaagc tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg
    10381 catgtaaagc actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc
    10441 cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc
    10501 gtagtgcctc atcagtggtt acaccgaact gctgccacgg ctgcggcgca aaaaaatcgc
    10561 ccttatacca ttcaccccgc gtagcggttt gtagcacatc aaggccgaga atcggctcgc
    10621 gaatgccgtc acgttccgct accagtccgc cgctaaagaa actgccgctc gcaagcaccg
    10681 caaatcgtgg gcgcagcggg atatcggcgt gattgcgcgt ccagatttca ttcactacac
    10741 catttttaca ggtcactttt ttcacttcat cgcccggcat ccacacgccg ccctggcgca
    10801 caaactggcg ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg
    10861 gcaaaagcat caatgaacaa ggtagttttt cattcaacca atgccacagt ttgtcatcgg
    10921 ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt attggcgacc ggaataagcg
    10981 catcaagtaa caacggccag ttttcttcat tatcaaggaa gcgggcaata ttcaccgccc
    11041 ggaactcggt ggcgttattg cgcagcacat ccagttccgg cagttcaatt tcagcggttt
    11101 caactttaag atccaattcg cgcaatgaag ccgccgcaag atgcgcctga aaatccatca
    11161 ggccgctaat tcccactaca catattttct tcgcgggcag cggccagaca gggacttctg
    11221 gcgaacttag ccaggttgag cgcagagtgc cgagcggcgt aatccgctgg tgagccagtt
    11281 ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga
    11341 gatcgagcac gcgttgaggc ccgagaagag tgtaaggatg ggcaggtgcc tgctgacgca
    11401 aagattccag tccactgtga atgtctgtca ccggttgacc atccggcaga tggctcagca
    11461 aatccagcga tccagatgag aaatggagcg cgctttgacc acgagtgaca atggcacagc
    11521 gcaggccgtg tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa
    11581 tgacagtatc aaagcgcatc tttctgctcc ttctccagac cacacaatcc ctgataaacc
    11641 cagcgggtaa attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc
    11701 cagcgttcgt ttaggaaggt ggaaagttgc tcaatagatt gcgcggacgt cgtgacgtta
    11761 aaacgttgca gcagtccggc agcgcggcag gcgcagagct cgccctggca ggtgcccatc
    11821 cccacacggg tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg
    11881 tactgcactt cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg
    11941 ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc
    12001 ggggcaggta gggagatgac tttacgcaag gtaacttcag cgggttcctg tgaaccagga
    12061 agtgccagat cggcggtcgt acaggggcgc gtgttgccca gtttgcggca tacggcatcg
    12121 gtagcccatt ccgccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaac
    12181 ccgtccagac catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt
    12241 ccgctcggat cgtcatcgct ggcaaccagc gggcgtacgc cagagtaggc ccgcaaaatg
    12301 cgcgttttcg ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacttct
    12361 tctgccgtta ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca
    12421 atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt cggaaggttt gcggcagcgg
    12481 ttgatcacat gctgattaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac
    12541 atgcgaatgc gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta
    12601 actacgacag gtgcatgaag tgcctgaatt tcgccggtga gatggttacg tacacgaacg
    12661 ccgcacaccg ccgcgccttc acgaatcagc cccgtgactt catgagcggt aaggataacg
    12721 gcaccgtgtt ctttggcatc cagcatgttt gctgctgtca gacgaaatgg atcaacggtg
    12781 ccatccggaa ctttcaccgc gccaatcagt gccgggttaa cggcaggttc gataatgcgc
    12841 gcttgctgcg gatcaatggc ttctgcgctg atccctgctt cttcgcaggc gcggataaaa
    12901 gtggcctgga aggagaggtc atcttccggc agggtgataa acaggccgtt ggttggttct
    12961 acgcagtgac gtgcaatgcg tttcaggatc tggttttcac taatacattc gcgggccgat
    13021 tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg
    13081 gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag
    13141 tcgcgggcaa tcccggctcc cgttgcgcca ccgccaatga taatcacgtc acttgattgc
    13201 gagtcgcgag ttttcattgt tatccctcag aagttcgttt tttaccattt agccatagta
    13261 aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt
    13321 tcatgcgtca ttctgaacat ttcaaccatc gtatttaata atgtgtgcgg taattcacat
    13381 ttaatttatg aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctcccact
    13441 gaatcaggct gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat
    13501 ttttaaacca gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg
    13561 tcgattgcgc tggcaaattt tcctggggat attctttggc tatgctgctt actatttggt
    13621 tcgtaagaac tttgcgctcg ctatgcctta tctggttgag cagggattct cacgcggtga
    13681 tttaggtttt gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg
    13741 ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc
    13801 ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt
    13861 tgtactgttg ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac
    13921 tatggtgcac tggtggtcgc agaaagaacg tggcggtatt gtgtcagtgt ggaactgtgc
    13981 gcacaacgtc ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa
    14041 tgactggcac gcggcgctct atatgcccgc tttctgcgcc attctggtgg cactgttcgc
    14101 ctttgcgatg atgcgcgata ccccgcaatc ctgtggcctg ccgccgattg aagagtacaa
    14161 aaatgattat ccggacgact ataacgaaaa agcggaacag gagctgaccg cgaagcagat
    14221 cttcatgcag tacgtgctgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt
    14281 cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa
    14341 gcatttcgcg ctggataaat cctcctgggc ctactttctt tatgaatatg caggtattcc
    14401 gggcaccctg ctgtgcggct ggatgtcgga taaagtcttt cgtggtaacc gtggggcaac
    14461 cggcgtcttc tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc
    14521 aggtaaccca accgtcgata tgatttgtat gattgttatc ggcttcctga tctatggccc
    14581 tgtgatgctg atcggtctac atgcgctgga actggcaccg aaaaaagcgg caggtacggc
    14641 agcgggtttt accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt
    14701 tggctacacc gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag
    14761 cattctggcg gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca
    14821 attactgcaa aaacgcaacg gaggctaatg gcatgaaatt gacgctgaaa aaccttagca
    14881 tggcgatcat gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg
    14941 aaaaaatagt catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag
    15001 caaaagcgat ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca
    15061 aagacgacca tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg
    15121 atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg
    15181 atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc
    15241 agacttatcc ggggcgtttc ccaatgggta aatctgactt ccgggtgcac acctttgaag
    15301 aagagattga atttgttcag gggttaaacc actctaccgg gaaaaatatc ggtatctatc
    15361 cagaaattaa agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc
    15421 tggaagtgct gaagaaatat ggttacaccg gtaaggacga caaagtttat ttgcaatgtt
    15481 ttgatgctga tgagctgaag cgtattaaga atgagctgga acctaaaatg ggtatggatc
    15541 tcaatctggt acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg
    15601 acggaagctg ggttaattac aattacgact ggatgtttaa gccgggtgcc atgaaacagg
    15661 tggcggaata tgctgatggt attggtccgg attaccatat gttgattgag gagacatcgc
    15721 agccgggtaa tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag
    15781 tgcatcctta taccgtgcgg tcagataaac tgcctgaata cactactgat gtgaatcagt
    15841 tatatgatgt tctgtataac aaagcgggtg taaatgggct atttactgac ttccctgata
    15901 aggcagtaaa attccttaat aaagagtaaa ttaactaccg gatggcactt atgtgctatc
    15961 cgataccact cttcattaaa gtctgtgggt tatccttaat tgcaattatt tagagcgaga
    16021 tgacttgttc ccggcataag atactcgtca atacaacacg tattattaag attttcctca
    16081 gctaaacatc tatagtcatt gtcgttaata aaaaacgaca ggcgataggg tatggcagtt
    16141 tcagcaaaac acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag
    16201 cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag
    16261 ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga
    16321 ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg
    16381 ccgaagattg tttttggtga agcggtgaaa attgagggtg aatggcgcgc gttgctggtg
    16441 actgaagata tggcggggtt catcagcatt gctgactggt atgcccggca tgcagtatcg
    16501 ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa
    16561 atgcatagcg ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca
    16621 gaaggtaagg cagaagctgg atttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgt
    16681 gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc gatccccaaa
    16741 gcggactggg agcaggtgaa agcatattac tacgctatgt aatcgttgta ggccagataa
    16801 gacgtataag cgtcgtatct ggcgttaaaa ccaattcaca tctcgatttc aatatcgcct
    16861 tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaagctaa cggttcggca
    16921 atccagtcca cctggcctgc gaccagtcgg gtgcgacagg agccgcagta accttcgcgg
    16981 cactggtact caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca
    17041 tcctggcaca gcagttgtgt gccagtaatg cgcagggtaa cgcgggccat cagagctgga
    17101 agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga
    17161 cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccacg
    17221 ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat
    17281 tggtgatgta ttcaacgtac tggcagagaa tgtctttatt cagaccaatc atcgaaccat
    17341 cacggaacag ataatccgcc cagtctttct cttgctgtgc cgcctggaca aacaggtcat
    17401 agcactcctg cttacattct tcggcaattt ccgccatctc cggatcatcc gcgccgctgc
    17461 gcagcagatt cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca
    17521 ggcgaataat tttggcgtta ccttccatca gctcacgttc agcgaatgcg aaagagcagg
    17581 caaagctgac gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg cagagataca
    17641 gttttttctt cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc
    17701 cttcgcccag cagatgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga
    17761 tcccctccgc acgtttctgg atctgctcgt tggtaacgat atcgtcaaag acaacagacg
    17821 gatcgttaac gatattacgg atgatatggg tataggagcg ggaatgaatc gtttctgaga
    17881 acgcccaggt ttctacccag gtttccagtt ccgggatcga gatcagcggc agcagtgcca
    17941 cgttcggact gcgaccctga atggaatcca gcagcgtctg atatttcagg ttgctgataa
    18001 agatgtgttt ttcgtgttca ggcagtgcct ggtaatcgat acggtcgcgg gagacgtcaa
    18061 cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat
    18121 atttttgctg atcgtagcga gccacgttga ccggctgacc aaagaacatt ggttctttga
    18181 gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gaatgtgtcc tgtggtttta
    18241 ttacccctca cccgtcccct ctcccgcaag gggagagggg acggtttggt gcaaagactg
    18301 ggggattatc agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggcacc
    18361 agatcgtcct gcgcgtcttc tgcgccgtca cgggtgttct gataatacag cgttttgacc
    18421 ccgaatttgt aggcagtgag caggtctttc agcaactgct gcatcggcac ttttcctgac
    18481 gggaaacgtg acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc
    18541 atgataccca ccagttgcag gtagccatcg ttacccggca tttcccacaa cagctcatag
    18601 gcgtcgtgca gatgctcgta gtccggcacc acctggcgca gaataccgtc tttcgacgct
    18661 ttgatgctga cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgcgaa
    18721 gaagtctcgg acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt
    18781 gactcacgca gtgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct
    18841 ttcttatagg tatcgatcgg caggatccct ttcgcgtatg tggtttcgtt aaaccacggg
    18901 cacgcgcctt gctctttcgc cagctcatta gacgctttca gcaggtaata ctgaatggct
    18961 tcgaaggttt tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc
    19021 gccaggtagt aagcgaagtt gatcacacca atacccagcg tacgacgacc catcgctcca
    19081 cgtttggcgg ccgggatcgg gtaatcctga taatccagca gcgcgtcgag tgcgcgtact
    19141 gccagaattg ccagctcttc cagttcatcc aggctgttaa ttgcgcccag gttgaaagca
    19201 gacagcgtac acagtgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc
    19261 ggcagggcga tttccaggca caggttagac tggcgcactg gcgcgatggc cggatcaaac
    19321 gggctatggg tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt
    19381 tcctgcatca tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg
    19441 tctttctcat atttggtata cagacgttca aactcttcct gatcggcgaa gaacgcgtcg
    19501 tacagccccg gtacgtcgga cgggctgaac agagtgatat cttcaccttt cagcagacgg
    19561 gtatacatca gtttgttgat ttgaaccccg tagtccatat gacgcacgcg gttgccttcc
    19621 acaccacggt tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag
    19681 aacagcgttg ccgcaccgcc gcgcacaccg ccctgagagc aggatttcac cgctgtctgg
    19741 aaatgtttgt agaacggaat acaaccggta tggaacgctt cgccaccgcg aatcgggcta
    19801 cccagcgcac gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtattta
    19861 acaatcgcgc tggaggtggc gttaatggaa tccaggctgt cgccgcactc gatcagcacg
    19921 catgagctga actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa
    19981 attttaaatg tggaaaccgc gtcgtaaaaa cgcttcacgt attgcaggcg cgtttcacgc
    20041 gggtagttcg agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag
    20101 atttcgccgg tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca
    20161 taagagaagg tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct
    20221 tccgtgtagt cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccaca
    20281 tggtcgtaca gcgcaggcgg ctcaaactga ccgtaggctt ttttacgcag gtggaagatc
    20341 gccagacgtg cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggca
    20401 gccttgataa tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg
    20461 gagcgcagct cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga
    20521 acgcgatgga ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc
    20581 agattctgat tcatgtatgt cgtacctgtt tttcaatagc ttcatgcaga ctacctgtag
    20641 tgagggaagc tgctaagaac actatatata gggggtatgt ttgggattca ctgcaagata
    20701 gtgtgaaact ggccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt
    20761 ggcttccgcg caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat
    20821 ttgttaaaaa taaccgttcg gtgaattctt gaacggtaaa ataggatagg taaattgata
    20881 tagatggttt attaatcttg ttgctaatga ttgccaaaat tgatctaggc acaagtcgtc
    20941 acataatctg atgtatctgt tgtggtcaat atataagcga atgtaagcga ttgttgagtc
    21001 ggaaatggtt gttt
//
LOCUS       DAPQZD010000075        20941 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPQZD010000075 DAPQZD010000000
VERSION     DAPQZD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.075"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            115..972
                     /gene="focA"
                     /locus_tag="RO454_002706"
     CDS             115..972
                     /gene="focA"
                     /locus_tag="RO454_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDY0934519.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1027..3309
                     /gene="pflB"
                     /locus_tag="RO454_002707"
     CDS             1027..3309
                     /gene="pflB"
                     /locus_tag="RO454_002707"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDY0934520.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3501..4241
                     /gene="pflA"
                     /locus_tag="RO454_002708"
     CDS             3501..4241
                     /gene="pflA"
                     /locus_tag="RO454_002708"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDY0934521.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4338..5486)
                     /locus_tag="RO454_002709"
     CDS             complement(4338..5486)
                     /locus_tag="RO454_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934522.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            5800..6426
                     /locus_tag="RO454_002710"
     CDS             5800..6426
                     /locus_tag="RO454_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDY0934523.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(6462..7325)
                     /gene="dmsC"
                     /locus_tag="RO454_002711"
     CDS             complement(6462..7325)
                     /gene="dmsC"
                     /locus_tag="RO454_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDY0934524.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(7327..7944)
                     /gene="dmsB"
                     /locus_tag="RO454_002712"
     CDS             complement(7327..7944)
                     /gene="dmsB"
                     /locus_tag="RO454_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDY0934525.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(7955..10399)
                     /gene="dmsA"
                     /locus_tag="RO454_002713"
     CDS             complement(7955..10399)
                     /gene="dmsA"
                     /locus_tag="RO454_002713"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDY0934526.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(10699..11691)
                     /locus_tag="RO454_002714"
     CDS             complement(10699..11691)
                     /locus_tag="RO454_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDY0934527.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDVRRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLLDAINFNPLAESVHKLSID"
     gene            complement(11761..12102)
                     /locus_tag="RO454_002715"
     CDS             complement(11761..12102)
                     /locus_tag="RO454_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0934528.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            12207..12728
                     /locus_tag="RO454_002716"
     CDS             12207..12728
                     /locus_tag="RO454_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0934529.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLDQLRNNRGN"
     gene            12733..13155
                     /locus_tag="RO454_002717"
     CDS             12733..13155
                     /locus_tag="RO454_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934530.1"
                     /translation="MKTNTSDFTITISKNDQALIDNLKYLAEKHNNGRIAINLRHITG
                     VTIVTSYWHLYLIAKSVLENKDSVVNGRDIDTLPAQIKPIAYQLRNRFKNEGGIIFDF
                     PAAVAVSFSRGDILHSFRFALSGDETLWQLEDAGSSPA"
     gene            complement(13162..13353)
                     /locus_tag="RO454_002718"
     CDS             complement(13162..13353)
                     /locus_tag="RO454_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934531.1"
                     /translation="MSEDKKVVNLLSELSAALDNINPPRVKTTGDKDALNTEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            13491..13841
                     /locus_tag="RO454_002719"
     CDS             13491..13841
                     /locus_tag="RO454_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HDY0934532.1"
                     /translation="MKLQRNSSQQRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVD
                     RLLHGQSFIKQADL"
     gene            13852..14130
                     /locus_tag="RO454_002720"
     CDS             13852..14130
                     /locus_tag="RO454_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934533.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARKTNGDEPYIEIICMDSEAEIQKAD"
     gene            14142..14384
                     /locus_tag="RO454_002721"
     CDS             14142..14384
                     /locus_tag="RO454_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HDY0934534.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            14381..14494
                     /locus_tag="RO454_002722"
     CDS             14381..14494
                     /locus_tag="RO454_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934535.1"
                     /translation="MSIFMSWIVLIISVVCAIGIVRIIHSVKKIERFFTGE"
     gene            14588..14998
                     /locus_tag="RO454_002723"
     CDS             14588..14998
                     /locus_tag="RO454_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934536.1"
                     /translation="MHAKEEGIIRALKEISKTENEVAKKAIANNHMDVATHTLIVARV
                     TAEAAEIIAKQDAELAVLRTQPVTGLDLSNTGRLIYTIGSELQRYTIIAGLQDKYLIT
                     PHPIRESEILTNLRLIERSQVAFIDDAQCTVFNA"
     gene            15022..15225
                     /locus_tag="RO454_002724"
     CDS             15022..15225
                     /locus_tag="RO454_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934537.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            15222..15488
                     /locus_tag="RO454_002725"
     CDS             15222..15488
                     /locus_tag="RO454_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDY0934538.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            15485..15784
                     /locus_tag="RO454_002726"
     CDS             15485..15784
                     /locus_tag="RO454_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934539.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAALALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEVPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            15796..16413
                     /locus_tag="RO454_002727"
     CDS             15796..16413
                     /locus_tag="RO454_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDY0934540.1"
                     /translation="MHTQKNRLPCRNQSGYISAAPHKTGAGFRSLLTTQAHNRASGFF
                     VRTVLPRFFRVRIMAGRTGPTSVGPGSCVAGTANLVRLATHSFAALDGEFSQLTTQGA
                     TPWQTANSTALSRSVVTSRLKSTADFPAHHASRKSCTSICCMSAATHYQTFIPPLFSA
                     IWRMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSVTRGLPQ"
     gene            16410..16775
                     /locus_tag="RO454_002728"
     CDS             16410..16775
                     /locus_tag="RO454_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934541.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            16782..19604
                     /locus_tag="RO454_002729"
     CDS             16782..19604
                     /locus_tag="RO454_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDY0934542.1"
                     /translation="MTAEYLRDWQQPRHAVGREGTGIPAPESALSSWLDAYRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDSTDGRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQGQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            19681..20640
                     /locus_tag="RO454_002730"
     CDS             19681..20640
                     /locus_tag="RO454_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDY0934543.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVAPAIREVCGVNATFIADGVPQFALVNGLYAMDKE"
     gene            20645..>20941
                     /locus_tag="RO454_002731"
     CDS             20645..>20941
                     /locus_tag="RO454_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDY0934544.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQDNQPSGVECNFYKIKKNIQ"
BASE COUNT         5599 a         5523 c         5059 g         4760 t
ORIGIN      
        1 aaaaaccatg cgagttacgg gcctataagc caggcgagat atgatctata tcaatttctc
       61 atctataatg ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa
      121 gctgacaacc cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggca
      181 ggtgtctata aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt
      241 gttttcatct caatcgcatt cgtcttctat atcacagcaa ccactggcac aggtacaatg
      301 cccttcggca tggcaaaact gattggcggc atttgcttct ctctggggtt gattctttgt
      361 gttgtctgcg gagccgatct ctttacttcc acagtgttga ttgttgttgc caaggcgagt
      421 gggcgcatca cctggggtca gttggcgaaa aactggctaa atgtttattt tggcaacctg
      481 gtcggcgcac tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat
      541 ggtcaatggg gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt
      601 gaggccgtct gtcttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt
      661 tattctggcc gcagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt
      721 gttgccagcg gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta
      781 atccgcgact tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt
      841 tctcacctga ccgtgatgaa cttcatcact gataacctga ttccggttac gatcggtaat
      901 attatcggcg gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac
      961 gatcaccatt aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg
     1021 tgttacatgt ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt
     1081 gactggcaga atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag
     1141 ggtgacgagt ccttcctggc tggcgctact gaagcgacca ccaccctgtg ggacaaagta
     1201 atggaaggcg ttaaactgga aaaccgcact cacgcgccag ttgactttga caccgctgtt
     1261 gcttccacca tcacctctca cgacgctggt tacatcaaca agcagcttga gaaaatcgtt
     1321 ggtctgcaga ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg
     1381 atcgaaggtt cctgcaaagc gtacaaccgc gaactggacc cgatgatcaa aaaaatcttc
     1441 actgaatacc gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg
     1501 cgttgccgta aatccggtgt tctgaccggt ctgccagatg cttatggccg tggtcgtatc
     1561 atcggtgact accgtcgcgt tgcactgtac ggtatcgact acctgatgaa agacaaactt
     1621 gcacagttca cctctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc
     1681 cgtctgcgcg aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct
     1741 gcgaaatacg gctacgacat ctctggtccg gctactaacg ctcaggaagc tatccagtgg
     1801 acttacttcg gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt
     1861 cgtacctcca ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc
     1921 gaacaagaag cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc
     1981 ctgcgtactc cggaatacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct
     2041 atcggtggta tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg
     2101 aacaccctgt acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa
     2161 aaactgccgc tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg
     2221 cagtatgaga acgatgacct gatgcgtccg gacttcaaca acgatgacta cgctattgct
     2281 tgctgcgtaa gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac
     2341 ctggcgaaaa ccatgctgta cgcaatcaac ggtggcgttg acgaaaaact gaaaatgcag
     2401 gttggtccga aatctgagcc gatcaaaggc gatgtcctga actatgatga agtgatggag
     2461 cgcatggatc acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc
     2521 cactacatgc acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac
     2581 gttatccgca ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct
     2641 gcaatcaaat atgcgaaagt taaaccgatt cgtgatgaag acggtctggc tatcgacttc
     2701 gaaatcgaag gcgaataccc gcagtttggt aacaacgatc cgcgtgtaga tgacctggct
     2761 gttgacctgg tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct
     2821 atcccgactc agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt
     2881 aacaccccag acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac
     2941 ggtcgtgacc agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct
     3001 tacgctaaag atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac
     3061 gacgaagttc gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca
     3121 tccatcgaag gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac
     3181 gcgatggaaa acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta
     3241 cgtttcaact cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa
     3301 tctatgtaat tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg
     3361 aaagtggggc cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga
     3421 ctgccaaaac agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc
     3481 acatctggag aaacaccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg
     3541 gaaccgtaga cggcccgggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct
     3601 gcctgtattg tcataaccgc gacacctggg atacgcatgg cggtaaagaa gttaccgttg
     3661 aagatttgat gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg
     3721 ttaccgcatc cggcggtgag gcaatcctac aagctgagtt tgttcgtgac tggttccgcg
     3781 cctgcaaaaa agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg
     3841 atccggtgat tgatgaactg ctggaggtaa ccgacctggt aatgctcgat ctcaaacaga
     3901 tgaacgacga gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg
     3961 ctaaatatct ggcgaacaaa aatgtgaagg tgtggatccg ctatgttgtt gtcccaggct
     4021 ggtctgacga tgacgattca gcgcatcgcc ttggtgaatt tacccgtgat atgggcaacg
     4081 ttgagaaaat cgagctcctc ccctaccacg aactgggcaa acacaaatgg gtggcaatgg
     4141 gtgaagaata caaactcgat ggtgttaaac caccgaagaa agagaccatg gaacgcgtga
     4201 aaggcattct tgagcagtac ggtcataagg tcatgttcta atttccccgt aaagcggcaa
     4261 ctttattgag ttgccgcttt tttgtgcaca gaaaagcaga gtgtgaaaca gtaagttcac
     4321 actctgcttg aattcattta cacgtgagcc acgggtttcg gcgtatggcc ggcgttgcga
     4381 agcagcatca gcaaatagat aaacgatacg ctggcgatca tgataaacaa taaattatcg
     4441 gagaaattct gcattagcat agcggtaaat gacgggccaa gcaggcttcc cacggtatag
     4501 ctcaacagta aggcctggtt catcgccacc agttgatgat gttcaacttt ctcacaagcc
     4561 caggccatcg ccaccggata tagcgtaaag ccagcggcac cgaggatgaa taacgctggg
     4621 gccatcgccg cctggctaag catcgcaata ctgccgagaa tgacgacaaa gacctgaaca
     4681 cgcaacacca gcagtcgacc aaacttatcc gccagacgtc caatcggcca ttgtccgagg
     4741 atacccgcac tgaccagtac cgccatccag aaaccaatgc tggcattgct cacccctttg
     4801 tgattgaggt acagcggcat caggccatat agagatccca gcacgattcc tgagataatg
     4861 cagccattca cgccaagccg cgcctgacgg agttttagca ttgacgtaat tgacgtcgaa
     4921 tcatggtttt cagtctgctg attcaggaca cgcgtaaaca acagcggtaa gattcctgcc
     4981 aacgtcaaac ctgtaaccca cggcaataca ctcatcaact cggttgagac tttgctgacc
     5041 agtaactggc ctaaaaacgt tccgacgtaa taaaccatca tatacgcagc aagcaaccgc
     5101 ccgcggttac gcgacgtccc actgcacatc agcgcactct caacaaccac ccaaatcatg
     5161 gcacagccga tgccagcgac aaaacgccac gccaaccaac tccagaatcc aatcatcagg
     5221 ccaaggccgg cacagccagc ggcaaaaatg aaggaggcca gataatagct gcggttaaag
     5281 ccaatgcgtt taatgacata ccctgtcagc aatgtaccga caaggttgcc ggtaaaatag
     5341 gatgagctga caacgcctac ctgccatgta gacatgtgtt cctgggcgag ccaaagcggc
     5401 acgagtgtat ttaacaccgc gatcgccaga gtcaacaaaa gcaggccaga cagcaaaagc
     5461 atgacaggcc gggtatacgt ggacatgaat taaaaccgtg aggaagttca aatttcatgc
     5521 gcatcatgcc accgccaaaa acattgtcaa tgggtgaaaa tacgcgcata tcgcagcggc
     5581 ggtattatcg atttatatta ctcattcata acgctaatgc tgaagagacg attaatctca
     5641 tctgattgtt tttattgatc taacacaaat ttcatacgct tcgttcagtc gaaaaatttc
     5701 atggtcacag caaactcata tttcccgtct atgctttcag agcaatgcat cgcgtctgac
     5761 cgatttagac gtaccactga ctctcgaaag gagaagagga tgaccaaacc gtatgttcgt
     5821 cttgataaaa atgatgctgc cgttttgctt gttgatcacc aggctggttt actttccctt
     5881 gtacgggata tcgaacccga taagtttaaa aataacgtgc tggcgctggg tgacctggct
     5941 aagtatttca acttaccaac tattcttacc accagttttg aaaccggtcc taacggccca
     6001 ttagttccag aactgaaagc acaattcccc gatgcacctt acattgctcg cccgggaaat
     6061 attaacgcct gggataacga agattttgta aaagctgtca aagcgacagg taaaaaacag
     6121 ttaattattg ccggtgtggt aaccgaagtt tgcgtggcat tcccggcgct ttcagccatt
     6181 gaagaagggt ttgacgtctt tgtcgtgact gacgcgtcag ggacctttaa tgaaattacc
     6241 cggcattcgg catgggatcg catgtcgcaa gctggtgcgc aattgatgac atggttcggc
     6301 gtggcgtgcg agctgcatcg cgactggcgt aatgatattg aaggactggc gacgttgttc
     6361 tctaaccaca ttcctgatta tcgtaacttg atgaccagtt atgacacgtt aacgaagcag
     6421 aaataaaagc gaaccggggc cgaaaccccg gttgttactg attaatttgc gacagccatc
     6481 cccacggtca tatgcaaacc atagaatacg ccacgaccga ttaattcccc tgccagaagc
     6541 agaatgaatg atacagaaag tagcggaacc gcaggctgat aaccttttag ctgtggtgca
     6601 atccacacgc acagggcaac ggccaaaagc acgatccgcc aggacatcag cgcaccatag
     6661 tccggcacca gcgctgcggc ctgctgcaca gagctatgaa tagtcgccag ctctgcgccc
     6721 tgcatcactg acatcacgcc actcactacc agtgccagaa cagaaatggc tggcagcaga
     6781 cgcatcgccc agccatcgac gccagccaga ctcagcaaca ggtaacccag taacgggccg
     6841 cccataaaca tcgtcaggaa gaagcccatc ggtgtccaga tgctgtacca ggtgggtact
     6901 gtatcgatgc tgttatacac acgcaccatc atccagacaa agactacgcc aagaaccatc
     6961 gtcaccacca gccaaagcgt acgcaatgcc ggtgaaagct ttttcagcat tgccagcaac
     7021 cagccgatgc cgcctacagc aaaaaagatc gaaccgctgg cgatttcgtt actgagtgct
     7081 gaagccccta cccgattgag cgagttaaaa gcgcgcatcg gtgaaccaag atgaagcata
     7141 gaggagataa agccaatgcc catcagcacc cataaaccca acatgcaggc gataacacgc
     7201 tgctgggctt ctgctcgcag gtcgcctttg agcagcgcca aagccagaac gataaaacca
     7261 cccgcgacac attgcccgaa gaccgtgaag atcatcagcg gccattcatg ccatccactt
     7321 cccatctcac acctccttcg ggtttgccag atagccagtg gtatccccgg ttgggcggct
     7381 gttggcattg ggtttgatca caatattcgg tttggtaaag tgagctcgcg gcaacggcgc
     7441 aacggccgcc agatcgccat gttttttacg cagctcgtcg atagggccga aatccagcgc
     7501 ccgtagcggg caggactcta cgcagatcgg tttttttccc tccgcaacac ggtcataaca
     7561 accatcgcat ttggtcatat ggcctttcgt ttcgttatat tgcggtgcgc catacgggca
     7621 agccatatga cagtaacggc agccaatgca tacatcttca tcgaccacaa caaaaccatc
     7681 ttcacgttta tgcatcgcac cgctcgggca gactttagta caagccggat cttcgcagtg
     7741 gttacatgaa atcgacagat agtaggcaaa cacgttctgg tgccagacac cgttatcttc
     7801 ctgccagtcg ccaccagcat attcataaat gcggcggaag ctgacttctg gcgtcaaatc
     7861 tttgtagtct ttacaagcca gctcgcaggt tttgcaaccg gtgcaacggc tggaatcaat
     7921 aaaaaatcca tactgggttg tcatcggtta ctccttacac cttttcaacc tgaacaaggt
     7981 ttgtatgtga cggattcccc ttagcgagag gagacggacg ttgagtggtc agtacgttaa
     8041 tacaaccgcc cttatcgaca cgttttgtat ccgggtcata ccaggcacct tcacccagtg
     8101 cgaccacacc cggcatcatt cgtggcgtca ctttcgcctc aatatgaacc tcaccacgat
     8161 cgttaaagat cctgacttta tcgccgttgt tgataccgcg tttttgcgca tcaagcgggt
     8221 tgatccacat ttcctgacgg caagctgctt tcagcacatc aacgttgccg taagttgagt
     8281 gaacgcgaga tttatagtgg aaacccgtaa gctgcagcgg atactgtttg ttcagcggat
     8341 cctgataact ttcaaaaccc ggcgtataga tcggcagtgg atcgatcaca tcaccttctg
     8401 gcaattccca ggtagcggca atgtcagcca gcgcctgcga ataaatctca attttccccg
     8461 atggcgtggt cagtggattt gcctgcggat cttcacggaa agctttataa gcaacgtgat
     8521 gcccttgtgg gtcgcgcttt ttaaaaatcc cctgcttacg gaactcttca aacgttggca
     8581 gttcaggaat cgcttcccgc gactgggcat acagatgccg catccactct tcctgggtac
     8641 ggccctcagt aaactgttgc tcaacgccaa gacgttttgc cagttcgctg gtcatttcat
     8701 agatggtctt acattcaaag cgcggtttaa tcacctgatc gttgaaaatc acgtaagaca
     8761 tattcccgca ggatgcatcc agtgcaaagt ccatctgttc tgaagcggtg cagtcaggca
     8821 gcaggatgtc agcatatttc gccgatgagg tcatgtggca gtcgataacc acaatcagct
     8881 cgcacttctt atcatcctga aggatttcat gggtacggtt gatttcagaa tgctggttaa
     8941 tcaggcagtt accggcatag ttccagatca ttttgatcgg cacatccagc ttatctttcc
     9001 cgcgcacacc atcacgcagc gctgtcattt ccgggccacg ttcaatggca tcggtccaca
     9061 taaacatcga aatgctggtc tggatcgggt tttcgagggt cggcatacgg acaaacggta
     9121 agctgtatga accttcacgc gcgccgctgt tgcctccgtt aataccaacg ttaccggtca
     9181 gaatcgccag catcgagata gcacgggttg cgatttcacc gttagcgtga cgctgcgggc
     9241 cccatccctg gctgataaac gccggtttgg tactaccgat ttcacgagcc aatttgatga
     9301 ttttgtctgc cggaacacca gtgatttgcg aggcccattc cggcgtttta gccacgccat
     9361 ctggcccttc acccagaata taagctttat agtgaccatt tttcggcgca ctggctggca
     9421 gtgttttctc gtcgtagcca acgcaatatt tatcgaggaa tgcctgatcc accaggtttt
     9481 cggtgatcat gacatacgcc agaccgttaa ccagtgcggc atctgttccc ggacgaatag
     9541 ggatccactc atcttcgcgc ccggcaccgg tgtcggtata gcgcggatcg atgatgatca
     9601 tgcgagcatt agatttctgg cgcgcctgtt caagatagta agtcacccca ccgccactca
     9661 ttcgcgtttc gccagggtta ttaccaaaca gcactaccag tttactgttt tcgatatccg
     9721 acgggctgtt accatctgcc cagccgccgt aggtatagtt caaaccttcc gcaatttgcg
     9781 cggaagagta gtcaccgtaa tggttcagat aaccgccgca gcagttcatc aggcgcgcga
     9841 ccagggtatt tcccggcggc caggagcggg tcatggtgcc acccagcgta ccggtgccat
     9901 agttcagata gatggattcg ttgccgtact ctttgatcag gcgctgcata ttggtcgcga
     9961 tgatgtcgta ggcttcttcc cagctaatgc gctcgaattt gccttcaccg cgcgccccga
    10021 ctcgtttcat cggatatttc aggcggtcgg gattgtaaac gcggcgacgc atggaacgac
    10081 cacgcagaca ggcgcgcacc tggtgcaggc cgtcatagtt gtcatcgcca gtattatccg
    10141 tttcaacata tttgatttca ccgtccacga cgtgcatacg tagcgggcag cgactaccac
    10201 agttaactgt acaggcgctc cagataacct tttcgtctga ttttgttgga atggcgctat
    10261 cgacagcgtg cgcaatccga ctaaaaggta atgttaatgc gctgctggcc attgccaggc
    10321 cgccgatcgc tgtcgttttt accaaaccac ggcgactcac ctcagcagcc aataccgcat
    10381 cagggatttt cgttttcata atggctcact caagcttgct cacgattgat tccagtaaca
    10441 aaccgtattg tgtaattatt tctatagcag atcgctatat aggcttgtat acatcgaata
    10501 atttgaaaat caaagagata ggtggatttt cactcgaaag tagtattact tgtaagtgag
    10561 gagtaattat tgttcggtat caaagggaga caaaaaaagc gcccgcaggc gctttaacgt
    10621 ttcttgcccc tcctgatgat ttacttattt ttcaacgaat aagccaaaac agggggaaca
    10681 aattaacaca aattataact aatcgatgga cagtttatgg acactttcag ccagcggatt
    10741 aaagttgatc gcgtccagta aaaagtccgg cgaaaaatga gcgtacgtca ttgtttgctg
    10801 aatcgttgcg tgccccagga ttcgttgcaa cgtaacaata tttccgccat tcatcataaa
    10861 gtgtgtggca aatgtatgac gcatgacatg cgtagcctgc cccttcggca aatccggttt
    10921 cacctcctga agaatctccc ggaacttcag ataatcaacc tgatacaata accccgttcg
    10981 acgggtttta atcagaggca cgaccgaatc cgccacagga accgaacggg attttccgtt
    11041 tttggtgtta aagaacgtca cccggttgcc aacgatatgc tctccacgta gttccgatgc
    11101 ttcactccat cgggcaccag tagaaagaca cagaatggca acacgacgca catcaccctc
    11161 cagtcgctca agaagccgtt caatctcatc atcagaaaga aaggccatct ctgtgttctg
    11221 aactttcagt tttcgtatac cgcgtacagg gttcgcgttg tgaaagacct cagcttcaat
    11281 cagcaccgtg aacatggtag acagtacgca taagtcacga ttaatactgg acggcattaa
    11341 tccggcctgt aatttctctg accgatattc aagcataaat tttcgcgtca tctgactggc
    11401 gcgaggatct cccatttcac gaacaacctt ccctagccta accctgtagc tatccccgta
    11461 agcctgatta cgcccatcaa gcatccacca cgcatcaagc agctctgata atcgccgctg
    11521 atcagctggc ttagcctgcc atggcttgtc atgaaagttc tgtaacacat acttctcaaa
    11581 ctcctgagcc tttgatttca gggcaaagat ttttcgaatc cgctttcctt ccgccccctg
    11641 cggtctgaca tcgacctgat aacgtccgtc tttgagttgc ttaatcgaca tgattagccc
    11701 ctccaacgga taaattaact aaacactcct tgcgatatgt aattcgctca atgtgtagag
    11761 tcagccaaca ttgcggcctg atcggggtga tttttgggta ataccgattg aaacctgatc
    11821 gccctccgac tgaatagatc catcaagaga gagagccgga gcaatctgcc cggcgacagg
    11881 tagcgtctta tcttttatta gccacagcat gtatttttca aagcgatcga cttgcagaac
    11941 cgcctcaacg atttttgctc tagcgttctt ttgccccgat tcgtaattcc ttatggttgc
    12001 caaagacaac ccagtaatat ccgcaaactg cttttgtgtt aacccttccg ccttacgtat
    12061 ctgacgtaac ttttttgcgt agtctattga caagataacc atttgagtat taacctctcg
    12121 cacaaagata accttttggt tatctttaca agccaaaaca aaccatcaca aggcgcgaca
    12181 agcgcaccaa cgcaagagga taacaaatgc gggacttcat tgccgataaa gagttatctg
    12241 accaccaacc acttcaggaa agtgacgcac tcagcggatc cattgatgcc gccaacgacg
    12301 acgagcacga agaaaacaag aaaaagcgtt cctacaaaaa aggagcgact ttgcggctgg
    12361 atggtccgat cgccgggctt tgctctctgg aaaaaggcgc ggcatatatt gggctaacaa
    12421 aatctgcact gcgcgtagcc attcatcgcg gtcaaatgcc gggacacaag acgcgcacaa
    12481 acccggaaga tgaaaactca gacggagtat ggtggtttaa cgccaaagag tgggacaagt
    12541 tggctgatga gctaccagag cacgagcccc cagaatggca caactggaaa agttactgga
    12601 cgtatgaccg ccagaaaagg aagttctctc cagctaataa agaagactgc cagaccatta
    12661 acggtaagcg agtttataca ggcagaagct caaaactgga ccaactcaga aacaacagag
    12721 gcaactaatc gtatgaaaac aaacacgtca gatttcacaa taactatttc aaaaaatgac
    12781 caagcattaa ttgataacct gaaatacctt gcggaaaaac ataataacgg acgaattgca
    12841 attaatttac gacacatcac aggagtaaca attgtaacca gttactggca tctatatcta
    12901 atcgctaaat cagtacttga aaacaaagat tctgttgtca atggaagaga tatagatact
    12961 cttcctgcac aaatcaaacc gatagcctat caattgcgta atcgttttaa aaatgaaggt
    13021 gggattatat ttgactttcc cgcagctgta gccgtgagct tctctcgggg ggatatactt
    13081 cactcatttc gttttgctct atcaggtgac gagacattat ggcagctaga agatgccggg
    13141 agcagcccgg cataaatcaa attaaaaatc aaccagttgt gtgacaggtg ggtatttacg
    13201 ctgaattttg tcagttacta acgccagtaa tatatgaatc tctgtgttta atgcgtcttt
    13261 atcacccgtc gtttttaccc tgggagggtt aatgttatcc agagcagcac taagttcact
    13321 caacaaatta acaacttttt tatcttctga catgaaaacc tcttaacaat ttaattaaac
    13381 ataagccagg acctgaaccg gccagtccaa gtcctagaca cacaaaaaaa ataccacaaa
    13441 tcaatacaag gtgacacgag tacaccttac cgaaggtaca gagttttaat atgaaactac
    13501 agcgcaattc ttcacagcag cgcttccgca acggtgcgga acgccatgct aaccgtttcg
    13561 ctaccagtgc atcacgtagc aactctcgct acagcctgag cgaaacacac gcaacgccgg
    13621 atggctaccc agtaaaacaa attggtgaac acacttggct gattgaaaaa gcaggaatcg
    13681 tggttcacag atgccagcgc aacccattta ccggaaaccg catttttgca ctgagcaacg
    13741 gcgacaatca gttcgggcag gatttcacat tatacgaagc acttcgcacg gttgatcgtc
    13801 tgcttcacgg gcaaagtttt attaaacagg ctgatttata acaggtgctt tatgaccaaa
    13861 gagcatgcac aaggtgtatt tatccgtttt attgattttc gcggtgaact gttattacgc
    13921 gcatcagcca ttgacggagt tgttccatcc gaaaaaaatg cagctactta cgtttatctg
    13981 aacggcacgc gcctgaccgt agaacttccg taccagactg tacacgaaat cattagcgaa
    14041 gcggaaaaag cacgtaagac taatggcgat gaaccctata tcgaaattat ttgtatggat
    14101 tcagaagcag aaattcagaa agcagattaa agggcgttgc gatgggcaaa gaatataaaa
    14161 ctctcattaa caaagcactt gagcgttttt attttcgctt aagtgcatca ggcgctcatg
    14221 ctgaacgtgc agcccgtgac tcattgacca gggcaatccg gagtctgtat gacgtggctt
    14281 tttacgctga tgatctggat gcacttaacg aactttccga gctgatctgt gccgcagaat
    14341 gcggggaaca tattgaaccg tataagctgg ggaatatcgc atgagtatat ttatgtcatg
    14401 gattgttctg attatttcag tggtctgcgc aattggcatt gtgcgaatta ttcattcagt
    14461 aaaaaagatt gaacgttttt tcactggtga ataacgatac aaataaaaca tcaaattaaa
    14521 taagaaaacg tgaaaaccat ccgtattaac ggaggtattc gcacacgcaa ataacggaga
    14581 tacaaaaatg cacgcaaaag aagaaggtat catcagagca ctgaaagaaa tttcaaagac
    14641 agaaaacgaa gtagcgaaaa aagccatagc aaataatcat atggacgtcg caacccacac
    14701 actgatagtc gcaagagtca cggcagaagc agccgaaatt atcgcaaaac aggatgctga
    14761 actggcggtt ctcagaacac aaccagtcac cggactggat ttgtctaaca ccggacgcct
    14821 tatttacaca attggctcgg agctacagcg atacaccatt atcgccgggt tacaggataa
    14881 atacctgatc actcctcacc ccataaggga atcagaaatt ctgacaaatc tccgcctgat
    14941 agagcgctct caagtcgcat tcattgatga cgcacagtgc accgtattta acgcatagag
    15001 ttactggaca aagggggcgc aatggcaatt aagcattttc ccgtcgttcg ctttacctcc
    15061 agagggcgcg aatacgaggt cgacgaacgc ctgattacca ctatcgacaa acatcgttcg
    15121 gaaaaggatg cacaccacat ctacctcact gacggcactt acttctgcgc cactaatgtg
    15181 gcgcgggtaa accttatccg acaggtacag gagccacgca gatgaccatt ctggactaca
    15241 tcgctactca tccggggtgt agcggcggag agatcgccgc agcactgaat actccaacca
    15301 cagccattaa tgctgagtta cgccaacttt ggcgcggcgg cttagtcatc agaacaaacc
    15361 gcagcacagg tggtcgcgct cgcaaaactg gaggccaggc ttcttaccac gtaaacccga
    15421 tgccgttcgg gtgtagcaat ccacttactc acatgtttaa ccagctactg aaggaagcca
    15481 gaacatgagc accatcaacc accagaagct acgcgaactg gcatttgccc tgcaacgaat
    15541 ggcaacacct caaaaattac tggcatttcg cgcaatgctc tcgccgtctg ctgcgctggc
    15601 attgctggat gagctggagc acgccagaac cacggctcct gccattcgcc tgacgctcca
    15661 tcatgaaatc gctgatttct gcgcgacatt ggaggtgcca ggcgaaccgg aaacgccgga
    15721 agcaatacag caagagctgc tgcaacgtat tgacaaggtt tttgattttt ttctgaacca
    15781 gtaagaaacc agagcatgca cacacaaaaa aaccgcttgc catgccgcaa tcagtcaggt
    15841 tacatttccg ctgcacctca taaaacgggt gccgggtttc gcagcctgct gactacacaa
    15901 gcgcacaacc gcgccagcgg tttttttgtg cgtactgtat tgccacgttt ttttcgcgtc
    15961 agaattatgg cggggcgtac ggggccgact tcggtcgggc cgggttcttg tgtagccggt
    16021 actgcgaacc tcgtacgtct cgccacccac agtttcgcag ctctggatgg tgagttttca
    16081 caacttacta cacaaggggc cacaccatgg caaaccgcaa acagcaccgc gctatcgcgg
    16141 agcgtcgtca catccagact gaaatcaacc gcagactttc ccgcgcatca cgcgtcgcgc
    16201 aaatcatgca catcaatatg ctgcatgagc gcagccacgc actatcaaac atttattccg
    16261 cctctgtttt cagctatctg gcggatgatc tgcacgagct tcaacagctc atccagcagc
    16321 aaaacaaact ccattaattc ctgttccggg cctttcctgc accttgcggc gggaggcctt
    16381 cgcacatctg taacaagagg attgccgcaa tgattctcgc caacgacttt cttgaatacc
    16441 tgctcaatac agaacgtgat cttgccgctc gcgtgcgtga tcgttatgac atgtacctga
    16501 aatccctgcc tgtaccgcag ctcgctgacg gaaagattgt tattgatggt cgctacatga
    16561 ttgacagcca cgagggaaat tacaggcttt accgcattga aggtggcacc ccgtccgtta
    16621 ttggcattta ccagcgccca tcctctgcaa tcgtcgatgt gattgccgac agcatccgca
    16681 tcacacatcg ccatgccgac acagaagaca ccgtgctgga aattcagcgg ctggctacag
    16741 tctgccgcga caccctgaat ggcatgacga agtaaatcac tatgacggca gagtacctca
    16801 gggactggca acaaccgcgc cacgcagtgg ggcgtgaagg aacggggatc cccgctcctg
    16861 aatccgcgct ttcctcctgg ctggatgcct accgggcaga gaacgagcgc cgccaggaaa
    16921 tggctgatgc ggcgttctca gccacgccgc tgggcaacct gattaataaa agcctggacg
    16981 cacaggaaaa acaggacaaa accatcacac tggcaggaga cgccagaaaa caggcacgtg
    17041 gcgcggtgga tgaagccatg gcctcgctgc gcctgctgcc gtcctatctg cgcgatccgc
    17101 ttattcgcca cctctccttc ctgcgcaaaa aacaggaagc cgatcgccgg aaaggcaaaa
    17161 agagctggca ggcggaacgc tatgcacgcg gaaccctgcg caaaatattc gaacgtctgg
    17221 atagcactga cggacgctgg ctgacaccgg gttatcgctc ccttgccgga cgcgaacgcc
    17281 tggacgattt gctttacctg ccgcagctca acaaacacca gatacagacg ctggccacca
    17341 tgacggcggc gatgttcagc agcaccttcg aaaaactctg cgatggcttt ggcgcgaccg
    17401 atggcgagct gaccatggat gtaaccctga aggcgtatca gatgctggcc cgcatggcgt
    17461 tacacctgca cgccatgcct ccacattatg acgcactgac aacagacaaa gaccggagga
    17521 acgaaccgga cacggagctg ctgccgggcg caatccttcg cctgacctgt gcggaatggt
    17581 ggaaacgcaa actgtggctg ttacgttgcg agtggagaga agaacaactc cgcgccgcct
    17641 gtctggtttc cagaaaaaca tcaccctatc tgagccagga cgcgttaagc gagtttcgcg
    17701 cacagcgcga gaaaacacgc gatttcctga aaagtttcat gctggaaaat gaagacgggt
    17761 tcacgattga tctcgagaca gtgtattacg cgggagtaag taacccggtt caccgtaagg
    17821 cagaaatgat ggccaccatg aagggactgg aacttctggc cgaagcccgt ggcgacaaag
    17881 cggtgtttct gactgtcacc tgcccgtcaa aataccacgc cacaacggag aacggtcatc
    17941 cgaatcccaa atggaacggg gccacaatgc gcgactccag cgattacctg gttaacacgt
    18001 tttttgcggc ggtccgcaaa aaactgaacc gcgacggcct gcgctggtat ggcatccgca
    18061 cggtggagcc tcatcatgac ggcaccgtgc actggcatat gatggtcttt gcacatccgg
    18121 aagaaatcga caccattgtg tcccataccc gcgatattgc cattcaggaa gaccgccacg
    18181 agctgggcaa tgatattact ccgcgcttta aggtggagta tgtcgacggc tcaaaaggca
    18241 cgccaaccag ctacatcgcc acctacatcg gaaagaacct ggacagccgc gccgtggatg
    18301 gcatcgaccc gaaaacgggc aagccacgcg tggaccacga aaccggaaaa tcaatggccg
    18361 agagcgtgga gcgcgccatc ggctgggcgc gccttcaccg ggtccgtcag ttccagttct
    18421 ttggcatccc ctcccgtcag gtgtggcgtg aactgcgccg ccttgccagc cagatggcac
    18481 gcaatccgga aggcccgcaa cggctgaaag atgatgcaat ggatgcggta ctcgctgccg
    18541 ccgatgccgg gtgttttgcc acctacattg aaaaacaggg tggcgtgctt gttccacgca
    18601 aggactacct gattcgcacc gcctacgacc tcgcagatga gctgaacgat tacggcgaac
    18661 agagcgtaca gatttacggg atctggtcac cactcatcgg ggaatcctcc cgtgtgtgca
    18721 cgcatccgga taactggaag ctggtaagac gtaaaccgga agcggaagac agcgcccgcg
    18781 aaaatggttt tgaccttcag ggcggccctg ccgccccttg gactcgtggc aataactgtc
    18841 cccgtgtaca ggaaacggac aacaacggga cagaacagcc ggaagaacgg ccagcaccgt
    18901 ggccgcagct ccctgacggc gttgaagtga acgaatggat gcgctcactg aaacgacacg
    18961 aacgccgggc gctgatgcgt tcgcttcgtg acaaacaggc aaaaaacagc agcgatgaaa
    19021 tgcagagctg gacacagagc cgcaaacagc agcggccttt gcctgataac cacgaattac
    19081 tcgctaaaga atggcgggag tctgctgaat ctctcggcct gcatatcggt gaacaacaga
    19141 tgcagcacct gttacggggc ggcagtctgt acgttgacgg cagcatcatt gcaccgcagg
    19201 gatttgaaat tgtacgcaaa ccggataccc gcccggacag ccgaatcacg cagctctggc
    19261 agcgcctgag ccgtaatcat ggcgtaagca gcacggagat ccgccataac ccggtcgcca
    19321 gctatctggc acagctgggg gcatcagacc ctgaagccgc cgcacgcctg gcatccacac
    19381 ttcagcaggg ccagaacacc atgaaaacac ccgttaccgt gctttctgac atgctgcgcg
    19441 ccatccgcga cgcagagcac gcacagagaa tcagtgaaac cactgaacgc gccagccgca
    19501 aagcagacct gctgcggggt ggcctgacca gtggaaacaa aaaacagaca gaaacgggac
    19561 ttacgaatcc cgtaaatgag caaaaaacgc gcagcgatat atgaagcgcg cacaaaacag
    19621 gcaaaaacgg gatttcagaa tcccgtaaac gattaattaa tcaacataag gaaaagcgac
    19681 atgaaaattt gtatcgacga cggctccacc aacatcaagc tggcatggac tgagaatggc
    19741 gaacgccgca acgccatcag cccgaacagc ttcaagtcgg aatggtctgc gccgttcggt
    19801 ggcatgcagc ccgcgaacta catgcttgat ggcgtgcgct atggttttga tccggttagc
    19861 gatcgctttg tccagacgac cgacacgcaa taccagtaca gcgatgtgaa tgtaatcgcc
    19921 attcatcacg cgctggtcaa atcaggcatc acgccacagg aggtggatgt ggttgtcacc
    19981 ctgccactga gcgagtattt cgacacaaac gcacagccgg acatggccaa catcaaccgc
    20041 aaaaaagcga acgttatgcg cccggtggaa taccagaacg gcgaagcatt cactatccgt
    20101 aacgtgcggg ttatgcctga atccattccg gctggcttta aagcactggc tgacatgagt
    20161 ccgtttgaat ccctgctgat tgtggattta ggcggaacca cgctggatgt ggcaaaggtt
    20221 caagggcaac tggcaggtat cagccaggtg ttttgcgatc cacacgtagg cgtttccctg
    20281 atggccgatg ccgtactgtc ggtgatggcc actaacggta tgcgcaccag tcaccacatc
    20341 gccaatacca ttatcgaaca tcgccatgat gaagcctggc tgcgccagca catccacaat
    20401 gacgcgcatt acgccagcct gatggcggtt attcgtgaaa aggaagaaac actgaaacaa
    20461 cgcgtgatcc gcgcgctggc ggttttttcg ggttacgggc gggtgatggt tgtcggtgga
    20521 ggggcggaga ttgtggcacc cgctatccgc gaagtctgcg gagttaatgc gactttcatc
    20581 gcggacgggg tgccacagtt tgctctggtt aatgggctgt acgcaatgga caaggagtaa
    20641 accaatgacg acaccaacca gacggataag tttctatctg aagcccgccg ccgtcaagag
    20701 tgaacaggag gcgtgcaatt acctcgatag cctgccagcc tccgaacgca gccgcgcgca
    20761 acgcgcggcc tttctggctg ggctagcgct cataaagcgc aacccagcat ttgcctactg
    20821 gatggccgaa tggccagagg acaacctccc cttcaatacc gttagcatca aggaaacata
    20881 ccaggataat cagcctagcg gagttgaatg caatttttat aaaataaaaa agaatattca
    20941 g
//
LOCUS       DAPQZD010000076        20881 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPQZD010000076 DAPQZD010000000
VERSION     DAPQZD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.076"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            36..698
                     /gene="atoD"
                     /locus_tag="RO454_002732"
     CDS             36..698
                     /gene="atoD"
                     /locus_tag="RO454_002732"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDY0934545.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            698..1348
                     /locus_tag="RO454_002733"
     CDS             698..1348
                     /locus_tag="RO454_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HDY0934546.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            1345..2667
                     /locus_tag="RO454_002734"
     CDS             1345..2667
                     /locus_tag="RO454_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDY0934547.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            2698..3882
                     /gene="atoB"
                     /locus_tag="RO454_002735"
     CDS             2698..3882
                     /gene="atoB"
                     /locus_tag="RO454_002735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDY0934548.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(3956..4732)
                     /locus_tag="RO454_002736"
     CDS             complement(3956..4732)
                     /locus_tag="RO454_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDY0934549.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4737..6386)
                     /locus_tag="RO454_002737"
     CDS             complement(4737..6386)
                     /locus_tag="RO454_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDY0934550.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKLGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(6387..10901)
                     /locus_tag="RO454_002738"
     CDS             complement(6387..10901)
                     /locus_tag="RO454_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDY0934551.1"
                     /translation="MGTGLANAEDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKDST
                     SHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAP
                     GSEWIGMQVK"
     gene            complement(10925..11548)
                     /locus_tag="RO454_002739"
     CDS             complement(10925..11548)
                     /locus_tag="RO454_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDY0934552.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(11545..13233)
                     /locus_tag="RO454_002740"
     CDS             complement(11545..13233)
                     /locus_tag="RO454_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDY0934553.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(13382..16009)
                     /gene="gyrA"
                     /locus_tag="RO454_002741"
     CDS             complement(13382..16009)
                     /gene="gyrA"
                     /locus_tag="RO454_002741"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDY0934554.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            16156..16878
                     /locus_tag="RO454_002742"
     CDS             16156..16878
                     /locus_tag="RO454_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDY0934555.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(17018..20776)
                     /gene="yfaL"
                     /locus_tag="RO454_002743"
     CDS             complement(17018..20776)
                     /gene="yfaL"
                     /locus_tag="RO454_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDY0934556.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDKT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         4775 a         5756 c         5209 g         5141 t
ORIGIN      
        1 tgtacccaca acggtgtatg caagagggat aaaaaatgaa aacaaaattg atgacattac
       61 aagacgccac cggcttcttt cgtgacggca tgaccatcat ggtgggcgga tttatgggga
      121 ttggcactcc atcccgcctg gttgaagcat tactggaatc tggtgttcgc gacctgacat
      181 tgatagccaa tgataccgcg tttgttgata ccggcatcgg tccgctcatc gtcaatggtc
      241 gagtccgcaa agtgattgct tcacatatcg gcaccaaccc ggaaacaggt cggcgcatga
      301 tatctggtga gatggacgtc gttctggtgc cgcaaggtac gctaatcgag caaattcgct
      361 gtggcggagc tggacttggt ggttttctca ccccaactgg tgtcggcacc gtcgtagagg
      421 aaggcaaaca gacactgaca ctcgacggta aaacctggct gctcgaacgc ccgctgcgcg
      481 ccgacctggc gctaattcgc gctcatcgtt gcgacacact tggcaacctg acctatcacc
      541 ttagcgcccg caactttaac cccctgatag cccttgcggc tgatatcacg ctggtagagc
      601 cggatgaact ggtcgaaacc ggcgagctgc aacctgacca tattgtcacc cctggtgccg
      661 ttatcgacca catcatcgtt tcacaggaga gcaaataatg gatgcgaaac aacgtattgc
      721 gcgccgtgtg gcgcaagagc ttcgtgatgg tgacatcgtt aacttaggga tcggtttacc
      781 cacaatggtc gccaattatt taccggaggg tattcatatc actctgcaat cggaaaacgg
      841 cttcctcggt ttaggcccgg tcacaacagc gcatccagat ctggtgaatg ctggcggaca
      901 accgtgcggc attttacccg gtgcagccat gtttgatagc gccatgtcat ttgcgctaat
      961 ccgtggcggt catattgatg cctgcgtgct cggcggtttg caagtagacg aagaagcaaa
     1021 cctcgcgaac tgggtagtgc ctgggaaaat ggtgcccggt atgggtggcg cgatggatct
     1081 ggtgaccggg tcacgcaaag tgatcatcgc catggaacat tgcgccaaag atggttcagc
     1141 aaaaattttg cgccgctgca ccatgccact cactgcgcaa catgcggtgc atatgctggt
     1201 tactgaactg gctgtctttc gttttattga cggcaaaatg tggctcaccg aaattgccga
     1261 cgggtgtgat ttagccaccg tgcgtgccaa aacagaagct cggtttgaag tagctgccga
     1321 tctgaatacg caacggggtg atttatgatt ggtcgcatat cgcgttttat gacgcgtttt
     1381 gtcagccggt ggcttcccga tccactgatc tttgccatgt tgctgacatt gctaacattc
     1441 gtgatcgcgc tttggttaac accacaaacg ccgatcagca tggtgaaaat gtggggtgac
     1501 ggtttctgga acttgctggc gtttggtatg cagatggcgc ttatcatcgt taccggtcat
     1561 gcccttgcca gctccgctcc ggtgaaaagt ttgctgcgta ctgccgcctc cgccgcaaag
     1621 acgcccgtac agggcgtcat gctggttact ttcttcggtt cagtcgcttg tgtcatcaac
     1681 tggggatttg gtttggttgt cggtgcaatg ttcgcccgtg aagtcgcccg gcgagtaccc
     1741 ggttctgatt atccgttgct cattgcctgc gcctacattg gttttctcac ctggggtggc
     1801 ggcttctctg gctcaatgcc tctgttggct gcaacaccgg gcaacccggt tgagcatatc
     1861 gctgggctga tcccggtggg cgatactctg ttcagtggtt ttaatatttt catcactgtg
     1921 gcgttgattg tggtgatgcc atttatcacc cgcatgatga tgccaaaacc gtctgacgtg
     1981 gtgagtatcg atccgaaact actcatggaa gaggctgatt ttcaaaagca actaccgaaa
     2041 gatgctccac cgtccgagcg actggaagaa agccgcattc tgacgttgat catcggcgca
     2101 ctcggtatcg cttacctggc gatgtacttc agcgaacatg gcttcaacat caccatcaat
     2161 accgtcaacc tgatgtttat gattgcgggt ctgctgctac ataaaacgcc aatggcttat
     2221 atgcgtgcta tcagcgcggc agcacgcagt accgccggta ttctggtgca attccccttc
     2281 tacgcgggga tccaactgat gatggagcac tccggtctgg gcggactcat taccgaattc
     2341 ttcatcaatg ttgcgaacaa agacaccttc ccggtcatga ccttttttag ttctgcactg
     2401 attaacttcg ccgttccgtc tggcggcggg cactgggtta ttcaggggcc gttcgtgatg
     2461 cccgcagccc aggcgctggg cgctgatctc ggtaaatcgg taatggcgat cgcctacggc
     2521 gagcaatgga tgaacatggc acaaccgttc tgggcgctgc cagcactggc aatcgccgga
     2581 ctcggtgtcc gcgacatcat gggctattgc atcactgccc tgctcttctc cggcgtcatt
     2641 ttcgtcattg gtttaacgct gttctgacgg caaccctaca gacagaagga atataaaatg
     2701 aaaaattgtg tcatcgtcag tgcggtacgt actgctatcg gtagttttaa cggttcactc
     2761 gcttccacca gcgccatcga cctgggggcg acagtaatta aagccgccat tgaacgtgca
     2821 aaaatcgatt cactacacgt tgatgaagtg attatgggta acgtgttgca agccggactg
     2881 gggcaaaatc cggcacgtca ggcgctgcta aaaagcggac tagctgaaac ggtgtgcgga
     2941 ttcacggtca acaaagtgtg cggttcaggt ctgaaaagcg tggcgcttgc tgcacaggcg
     3001 attcaggcag gtcaggcaca gagcattgtg gcggggggta tggaaaatat gagtttagcg
     3061 ccctacttac tcgatgcaaa agcacgctct ggttatcgtc ttggagacgg acaggtttat
     3121 gacgtaatcc tgcgcgatgg cctgatgtgc gccacccatg gttatcatat ggggattacc
     3181 gccgaaaacg tggctaaaga gtacggaatt acccgtgaaa tgcaggatga actggcgcta
     3241 cattcacagc gtaaagcggc agccgcaatt gagtccggtg cttttacagc cgaaatcgtc
     3301 ccggtaaatg ttgtcacccg gaagaaaacc ttcgtcttca gtcaagacga attcccgaaa
     3361 gcggattcta cggctgaagc gttaggcgca ttgcgcccgg ccttcgataa agcaggaaca
     3421 gtcaccgccg ggaacgcgtc aggtattaac gacggtgctg ccgctctggt gattatggaa
     3481 gaatctgcgg cgctggcagc aggcctgaat cccctggcac gcattaaaag ttatgccagc
     3541 ggtggcgtgc cccccgcatt gatgggtatg gggccagtac ctgctacgca aaaagcgtta
     3601 caactggcgg ggctgcaact ggcggatatt gatctcattg aggctaatga agcatttgct
     3661 gcacagttcc ttgccgttgg gaaaaccctg ggctttgatc ctgagaaagt gaatgtcaac
     3721 ggcggggcca tcgcgctcgg acatcctatc ggtgccagtg gtgctcgtat tctggtcaca
     3781 ctattacatg caatgcaggc acgcgataaa acgctggggc tggcaacact atgcattggt
     3841 ggcggccagg gaattgcgat ggtgattgag cggttgaatt aaccaataaa aacgcccgat
     3901 agcgaaagtt atcgggcgtt ttcttgaaca tcgacggcga aggttacccc attaatcacc
     3961 agtcaaaact tttcaccagc gtcagctcgc cagcattacg catcggtaca ataaaagttt
     4021 cctgtttctc attgaccgac ccttcatcgg tgatcagcgt cagttgggcc gtagttaatt
     4081 ccgtttcact gcgcccgccg aagtagttga tatacacctg ataacggccg tgaactggcg
     4141 ctggcatggc gaaaatctcg ggtccgtacc ctgtcgtgac atccatatcc agtgcgccac
     4201 tgtttttcag cactgtgtta ccgtaccagg catgttcacc gtcaggcgta acgacatgaa
     4261 gatcgaggtc ggtattgtcc gtatcccagg agagaaccag ccgtaaacgc gcgcgaatcg
     4321 ccccagcacc cggcgttgag taaaactgca ttttttgtcg gctttgccca tctgggctga
     4381 tgacctgcac gctgttgctg ccttcagtga aaatataagg gcgtgcaaaa gaaccatctg
     4441 attcaattct ctgcggcata ctggcaccgt tgaccaccag ccggccctgc tgaactttac
     4501 ccgccgcagc ataattcttt attttgccgc ggatctgcgc tgagatattt tgatcatctg
     4561 ccatgttgac ggacgacgcc gggtaattga tcgtttgtga aaaactggca tcttcgccct
     4621 ctgccggatg ccagccagag agcggtgcat caatctcgac ttcctgcaca ccttcactgt
     4681 gagcgacagg cgaaagcgcc accagtaaca acggaagaaa aatctttcgc atagcattag
     4741 tccattaata attgtcgggt cagcgtttcg atatagttct catcaagccc ggtgggataa
     4801 ccatcaaagg ccaggtgcag atactcatgg gttaaatcca gccgatcctg aagcgtaaag
     4861 aaattacgaa taaacagccg tttctgctgc cgatcggtat aggggaaacc tgagactaaa
     4921 cgacagaccg caaacacgtc tggttcgttg tatcccgtct caccttgtaa tatacgccgc
     4981 cactgcggca tttttttcgc cagccaggct ttcgctttgg gtaataactg gcaggttgag
     5041 cgtggcgctc cccagcggct aaggctgttg tcgggataag caaacgcgag tatctgatca
     5101 taacgctctc cctgccccgc ctgggcagta gcgtgccgcc aggaaagcgt cccttcggta
     5161 gcgcgactgc catgataatg aaccggatcg cccgcataga tgagatcctg cgtccaggcg
     5221 gtcatcgttc gcgcccccgt ggtagctggc gaagcagaaa cacgctgcgt agcgctgctg
     5281 tccgggatgg tcaggcaatc gccttcccga tcggcgtttt gctgtaaaaa cgtgcgaatc
     5341 gccacggtca tggctttcgc tgcctcagga ggcgtacttt tcgcctcgcg atccagaact
     5401 ctggcaacgt actcttcacg atcgagatgc gactgtaatt tcagtttgcc ctttaccgtc
     5461 agtaacgttg tctcaccgtg gctgacaaat gtcatgtggt taccattggc aaacgtcaca
     5521 cgatagcgcc cattgagcac gcctagtttg actgccgtcg tactcttttc ctcagtaact
     5581 ttcttcagcg gataacgagc aaacagatcc accagcacac attgcccact ggcaacttgc
     5641 gtcggcactg gcaaaacccg attaagcgcc gtcgcataac gggtcagtac cgttttgctg
     5701 gtgccactgc cagtaaccca cagcggtgta ccatcggtta accagccagc aaaaccaccc
     5761 tgacggattt cctgtttatc atcggcaaac cagctccagg ttttcacccg tacccgacta
     5821 ccgagcatgg atgcgacacc gattttcgcc tcgtccagca ccacatccag taagacttcc
     5881 tgagctttgt tttgcgcagg taacgtcgct aatgaatcaa gcagcgattt aaccgttacc
     5941 gaggtttctg gcttcagtgt agtaagagac gctaaccacg caggtgcctg acgctgttgc
     6001 cagtattgcc cccacatatc tgcgccaata tgcaaccgct gcggcgcaaa atagagtcca
     6061 caggaacgta ccagcgcgga atcgcgcgtt atgctctccc cagcctgaca acaataaacc
     6121 tcttccgggg agttgccgcg acactggtat ggctgctccg gttgatgggt atcctccagc
     6181 caggcgtaaa catacagttt ccacaaactc cctaatggcg tggtgagcgt gtcaggtaat
     6241 accgagactt tggtcacgcc tgatgacgac agctgataca gttgatcgtg ctgcgcacca
     6301 cgcagcgcca gctgtaacgg cgtctcctcc gcaagcgttg gcagagttac caacgccaat
     6361 agccagacga ttctgtacca gttcacttat ttcacctgca tcccgatcca ttcgctccct
     6421 ggcgcaacac tttgctgtgc aggtgcatag gaacgcacat aacgggcagg cggcagaacg
     6481 aattgccctt tttgcgagaa acgcagcaaa tggcggaagg tgaccgttcc cgtcagttct
     6541 tttaccggca ccatataggc cagttcaccc atttcattgc gcgctttttc cagcagttgt
     6601 ccttgctgtt ttccggcgtt cggtttattg accgaaatgc cccaggttgt acgctcaacg
     6661 tcagcccccg gcggcagcgg tacttccacc tgaccatagc gcagaacggc atcctgctcg
     6721 ctggtaagcg tgatttcatc gagatacagc gcatcgctgt caatctcatt gctggtgaca
     6781 ggctgtaagg taaagctcat ctcttcttca ccggggataa gccgatacaa ctggcgttca
     6841 acggtcaccg gaatattact ttgttgagcc gtttttgccg gttcacgcca gcggacctgc
     6901 acgttttgcg gcggtaattc gtcaccaaaa gagagaatgt ccggcacgcc ctgaccaacc
     6961 caacgccagt attcgcctcc tccggttaac ttatgtttgg cccatgcgcc cgcaggcgca
     7021 ggtaacacaa ccggaggcat cgtcgccata tatttcgcca gccagttcat ggccaacgcg
     7081 cgctcaatgg tggattgctc agcggttaaa ccacttaaaa tagcggccgc ctgggtagcg
     7141 tcgcctccac tattgagcag cacaaccgtg tgcgccagcg gttggttgct gtttgctgct
     7201 ttatcccacg catattgctg aacctgtggc atcacagtgg acttcaactg cgcttttttc
     7261 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc aggtgtatcg
     7321 aggatcatgc tgtcattgat atcgctggtg tcttcctcag agaaatcttc agttttagtt
     7381 ccgcgccggg cgatagcttc atccaaccct ttcaacaacg ttttgcacgg cagattcatc
     7441 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatgttgtc cgcctgctcg
     7501 gcataactgt cgagcatatg ttgccagtat tccggttgct gcgttacgcc gagcgcctgg
     7561 ctggcctgcc agtcggcgta ccatgcccat gccgtgagga aggcgtcgcc attgccatct
     7621 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
     7681 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
     7741 caggcgagac tgagcgggat cagacggcta ccggtgttga tcacgccacc ccacggttca
     7801 tcgactaacg catcaagatt gttacggaaa atctcctgcg gcgtttcgct actttgtagc
     7861 cggatattgc tcgcctgctc gggcaacatc agcgcattat cgccaccgcc gagcatgaca
     7921 tttttctgtt gttcaacggg ccacctgtta tccacaaaag acagttttgt gctaatgctg
     7981 tcctgtactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
     8041 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagca
     8101 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
     8161 cctgccgccg gtttgtcacc catgcgatat accgttggca tactccactt catgtagaga
     8221 tttttttccg aacgcaaata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
     8281 cgcgcggtga tgcgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
     8341 ccttgtttat cggttgtgag tgacggcatc caggccgcgg tatccacctc ttcacgccgt
     8401 ggacgttcca gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
     8461 tcgctggaga gcgcctgatc gtagctgata aacgacagac tggagctggt acgcacattg
     8521 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttccgg ttgcagcgcg
     8581 tagatcattt catcgaccac gcctaccgtt aactgcgcag aaacaggttt acctttcagc
     8641 gacgaggtta attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc
     8701 acccggatat ccagctgggg aacggcaact ttaatcccgg cgttctgaaa actgtactga
     8761 ccgttacgag tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcaccggt
     8821 acgcgggctt catactgggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc
     8881 gatagcagcg actgctgttc cacgcgatcg cgttccagcg tcaataatgc ttcgtcaatt
     8941 ggctccggga aggtaatcag catcttcgcg gtttcgccag gctgatacag tgttttatct
     9001 gccacgatat ctaccgtacc actatgcgac gtgctgtcct taccgctgac tgcatggctt
     9061 aacccggcga gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta
     9121 gcgaaattga cggtaaagga tttgccgcct gactgtagct ctccgctgtg actcgtgcgg
     9181 tcttcgagac gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca
     9241 taacggaaca caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa
     9301 tgcgccaggc cacgttcaat gaggatctct ttggtggtgg taactcgata cgccgcgccg
     9361 tcactggcgg agacggttaa caaataacgg ctcggtttat cggcggcggg gagattcaac
     9421 gccacatgac cattatcgtc ggaaaccgtt tcactgcctt ccatcgacac cgggaaacgt
     9481 ccggcataac gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc
     9541 acgcgggcat cttttaccgg ctcgccatcc gggtaaagca gttgcagttt gccgctgacc
     9601 gcttcgccag ttttgaactc ttttttgtcg agagctaaac caatctcaaa atggggtttg
     9661 atgtagtttg ccacgcgaaa actgctgcta tagacctgtt tgcggtaagc aagacgtaac
     9721 tcataacctc cggcgacggc attttctggc aggcggaaac tcccctgccc gccattacgc
     9781 gcatccagcg tgacattgac ggtttgcaac agactgccgt tggcgtccag caccgaaagc
     9841 ttcgccggtg cgctgacgat gggggatgaa tgcaacggat cgtggaactc gcgacccatc
     9901 actttaacat cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa gatatacaag
     9961 cgggtgttgt agatttcgct ttcgtagaaa aagttctcgg agacaaaaac gccgccttca
    10021 gcatccttac ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaggtaccg
    10081 ctgccatcgg tcacaccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag
    10141 ccgggcttcg cttcaccctg ttttttcccc gcggtccaca ccagaagctc gttacctgac
    10201 actttgctaa gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccaccc
    10261 accatcgctt cgacgagata cagtcccggc tcttgtttgc cgagaggaat atagatgttg
    10321 cccggctgcg gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc
    10381 tcgaccggtt ttgcctgcca tagtggatag cggaactgtt ccacaagtgg atattttttc
    10441 agcggtgaaa actggctgtt ctgtacataa cgggaaggtt taacaatggc attaccgagc
    10501 tgtaattcgg gtaacgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc
    10561 atcacgcggc gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagt
    10621 ccgtcgccca gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa
    10681 aatgccatcg ggtcaggaat acgatacagg cgaacgtcca cgccgccgta ctcttccatc
    10741 tgatagcgcc gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg
    10801 ctaaaactgc tgtcagcgag caaaaagaat gttcccccgg cgggcggcac atagttactg
    10861 gaaggaagcg aatcctcagc attagcaagc cctgttccca caagagacag ggacaagcac
    10921 gctgctatcg cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt
    10981 tgggtatcca tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc
    11041 cgttgtcagt tttcgtggcg cttccggtgt ggtagatgat gtaacgcccc atccagacca
    11101 ttaagtgctg ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagt gcctggttta
    11161 tgtcctggcc aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acataggggc
    11221 cggttttccc attcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg
    11281 tcatctctgg cggcaaatac tggttagata aaccgttact ttttagccat ttactgtcgt
    11341 gaactttcag cgcctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc
    11401 agcgtgggct tggcccctgg cggagctgtt cctgtgcaat gcgcacaaac cacgcgcgaa
    11461 acagcccgga ttgttcgaca tttagcattt cgctatgggc aacaacacaa tacagccagc
    11521 aaatcagcgc cagcagcccg tgtctcatag cggttgccag gttatgggta gccactgcca
    11581 ggcgacaccg ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc
    11641 gtccagcttc ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt
    11701 cggcagactg gtcagcgttt cgttacgcag cagtttcgcc attccttgtg gattgatata
    11761 gagcggaacg atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag
    11821 tgtttgcagg gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg
    11881 catcgccagc gacacgcgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg
    11941 cgctttcgga tactgaccgt atcgggaact cacttcgcga cgccagattt gcgcttcgcc
    12001 ctgctgagtc tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat
    12061 attttgcgta aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc
    12121 aacaaacagt ggggtttgca attttgagtc ttcataccag cacagccccg cgccaccgtc
    12181 taacgcccca ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat
    12241 accgtgtgaa tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccatac
    12301 cggcgtaaaa tcgaaactgg catctacgct ggcggattca tcgtttaacg ccagaaaact
    12361 gtgccagccg tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaatcgctg
    12421 gtagccaaac cccagcaacc tggcgctgac tacgatgcgc tggcgtactg gcgttttttc
    12481 ggcagtacgc gcttccaggc caaagcgggt ttcccagcgt tttttaccac tcaacaaatc
    12541 acttgcgatc gccgtggctt cggtatcctg ttggtcatct ttaaacagca tatccgaact
    12601 ggaaaaagcc aatattttgt cctgataagt cgcaaacatc aggacgttat tgccgttata
    12661 gcgcaactga taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt
    12721 acttaactgg ctgtcgctgg tagcggcaaa cagcaatggc tccagcagct tgcttaaacc
    12781 acttcgctgg atcagcacca tataatgaga aagatggcct tgtttatcgt gccacagtgc
    12841 cgcctgcgcg ggctggtcta agagtgacga aaagagctta tctttcagcg tgagatcgtg
    12901 ttcatagaca atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata
    12961 atagaaaacg aaatcttcgc tcagaacatc gtggagaaac ggaatggtaa ggagatcttt
    13021 gggaagctgg ctcagagagt cgctgtcgag aaagaggtcc ggctcattga gatcgatttg
    13081 cagattgttg tgcaccacca gtggcgacaa cgttttttct ggcccactgc cagagtattg
    13141 taatgcccat acgccagcgg atagcagcgc tattgcccca aaaccaacaa ggccataaaa
    13201 ccgccagcct ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc
    13261 attttttacg ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagacttta
    13321 cagcctggac ttttcaattc aaaaaaggga gatggctccc ttttggcatg aagcgatgaa
    13381 attattcttc ttctggctcg tcgtcaacgt ccacttccgg agcgatttca tcgtcccctt
    13441 ccgcggcact gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgtt
    13501 gcagacccac cacgttttca tcttccgaag tacggataag gattacgccc tgggtgttac
    13561 ggcctacgat gctgatttcc gacacgcgag tacgtaccag cgtaccggcg tcggtgatca
    13621 tcatgatctg gtcgcaatca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg
    13681 taaccttgat ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg
    13741 cggtacgttt accataaccg ttctgcgttg cggtgaggat tgcgccatcg ccacgaggca
    13801 cgatcagaga gacgacttta tcgccttcgc ctaaacgaat accacgaaca ccggtggtgt
    13861 tgcagcccat cgcacggaca gaagactctt taaagcgcac cactttacct tcagcggaga
    13921 acagcattac ttcgtcttcg ccgctggtca ggtcaacgcc gatcagctca tcgccgtcaa
    13981 ccagtttgat cgccacttta ccggcggtac gcagacggtt gaactcggtg aggacggttt
    14041 tcttcacagt accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca
    14101 ccggcaggat cgcggtgata cgttcgtcct gttccagcgg cagcaggttg acgatcggac
    14161 gaccacgtgc gccacgagta gcttctggca actggtagac cttcatcgaa tagacgcggc
    14221 cacggctgga gaagcacaga atatggtcgt gggtgttcgc caccagcagt cggtcaataa
    14281 agtcttcttc tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt
    14341 attcagaaag cggctgatac ttcacgtaac cctggtgaga aagcgtgacc accacatctt
    14401 cctgtgtgat cagatcttcc aggttgatgt cggcgctgtt agcggtgatt tcggtacgac
    14461 gtttgtcacc gaactgttca cgaaccagct ccagctcttc acggatcact tccatcagac
    14521 gatcggcgct acccagaata cgcaacagct ccgcaatctg atccagcagc tctttgtatt
    14581 cgtcgagcag tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg
    14641 cctgagcttg ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca
    14701 gccattccgg acgcgcagca tcatcgcccg cacgttccag catcgcagca acgttgccca
    14761 gctgccacgg attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga
    14821 tcagttcgat gatcgggtcg atgttcgcca gcgccacggc taatgcttca agaatatgag
    14881 cacgatcgcg agctttacgc agttcgaaaa tggtacgacg ggtcaccact tcacggcggt
    14941 gacgaacaaa cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca
    15001 atgccaccat gttaataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga
    15061 gcaccacttc accgaccgca tcgcgtttga cttcaatcac gatgcgcata ccgtctttgt
    15121 cagactcgtc acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa
    15181 ttttttcgat caggcgcgct ttgttcacct gatacggaat ttcgtggacg ataatggttt
    15241 cacgtccggt tttggcgtca acttctactt cggcacgggc acggatatat accttgccgc
    15301 gaccggtacg gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga
    15361 agtccggccc cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat
    15421 acgccagaca accgttgatg acttccgtca ggttgtgcgg cggaatgttg gttgccatac
    15481 ctacggcgat accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt
    15541 ccggaatttt ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat
    15601 cggccatcag ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg
    15661 cagagtcgcc gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg
    15721 agaatggctg cgccatacgg acgatcgtgt tataaaccgc caagtcacca tggggatggt
    15781 atttaccgat tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat
    15841 tgcctagtac gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga
    15901 catctggcag cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttca
    15961 gctcttcctc aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat
    16021 ccctctactg tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg
    16081 taaacctata cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac
    16141 ggagtagaag tgccaatgaa tgccgaaaaa tcgccggtaa accacaacgt agaccacgaa
    16201 gagatcgcta aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa
    16261 ccgctgcacc gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta
    16321 tttggcaaaa aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg
    16381 cgggagggcg cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaaa
    16441 ctgcacgcac tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac
    16501 gcggcaaaac atgccggaca gtatgacgtg gtgacctgta tggagatgct ggagcacgtc
    16561 cccgatcctc agtcagtggt cagagcctgt gcgcaactgg tgaaaccggg cggcgatgtc
    16621 tttttctcga cgcttaaccg caacggcaag tcatggctga tggcggtggt tggtgctgaa
    16681 tatattttgc gcatggtgcc taaaggtaca catgatgtga agaagtttat taaaccggca
    16741 gaattgctgg gctgggtgga tcagaccagt cttaaagaac ggcatatgac cgggctgcat
    16801 tacaacccta tcactaacac ctttaagctc ggccccggcg tggatgtgaa ctatatgctg
    16861 catacgcaga ataagtgagg ttgttgtttg gattcatccg gcaaccgcgc caagcctgat
    16921 gcgacgcttg ccgcgtctta tcaggcctac aaatgctccc cgtaggccag ataaggcgtt
    16981 tacgccgcat ccggcaacag tgccggtcag acagcgatta ccatttcact gtcatcgaca
    17041 aaaaccctgc catctgggca aaatcatcac tccctttctg ccacgccacg ctgccgcgca
    17101 gcgaaactcg ctgactgata ttgcccgtta ttcccacttt tatttcaccc cgttgcttca
    17161 ccgcatcgtc actgatagtg ctgccatcct cttcaatttc cgtcgcatgg ggatcgtgat
    17221 aataattcag atctaacgtt ggcgtgacac caacagcagt acgccattcg ctgtgtaaac
    17281 ccaaccgcat ctgaatatca tcaccctgcg attgtgacac gcgcgcatgg ttagcggcgg
    17341 taaaatcatc ctgctgcacg ccctgataaa tcacctgcgc ctgcggttca atcaccacac
    17401 cacgccccgg taaccactga taaccggctt ccagcgaggc gataatcccc gacgagtggt
    17461 aatgatccac tccatcgtct tgttcggaaa catcattgtt aaaccacgca tattgcagcc
    17521 agctatccaa ccaggccccc tgcttctgat taccgtgctg ataccagctt gaggtcagcc
    17581 caacggcata accgcggttc tggttatcgg cgcgagttcc ggtcatattc gagcggctgt
    17641 cgccctggtt atcgctgtag ccaccaacaa tcccaagcat ccactcgcca tcatcacccc
    17701 agtgcccgct aaacaggttg ccgctaagct gcaccgtaga agtgtcttca tgctgagcca
    17761 actgccctgc cgccgtgtaa tgataacgcc cgccgataac gcgtaaattc agcgtctgac
    17821 catcgccacc tgcgtgatcg cgtcgctcca tcacaaatgc ctgatttgcc gcacgtaggt
    17881 tattaaaata tccaccaact ttggcattca acaccggctg gtaagcaggc gtaggttcag
    17941 ggtcgggggt tgggtcggga tccggcgtgg gatcaggatc tggagtcggg tctggatcag
    18001 gcggcggcgt cggattgact tcttgtgatc gcagatacca gtcgttgtta tcttccacca
    18061 gcgtgtagtc atacgccccc atattgacgt agccgcttcc tgccagactg aactgcgcat
    18121 tgttttgaaa ctgcgtggga tcagctgcga aatcaaccac tttaatacct gtagatgtcg
    18181 gctcaccaat ccctgtaatg ggattaatca caaccgtggt attgccagca gtattaccgt
    18241 tcaacaccaa ctggtcgctt gccgagtcat cgccgtttaa ttcgctgtcg agcagtagcg
    18301 taccgccgcc ggtataatca ccatttaccg tcagcgtgtc gccagcgatg ccattttgca
    18361 gactgacagc accggacgta ttggtcagat tgccattaac cgtcacgtcg gaaagataag
    18421 tcaggctgtc agtttcaagg ttggtattaa gctcacctgt caacgtcacg ccatcggtgg
    18481 cattcaccag cgtaccgtcg ccgctaaaca gcgaggcatc cagcgcctgg ttcgtatcct
    18541 gctgcgtcaa tcgcaacaca gtaccatcgg ttatgtcgat atcgccgcca gaggtggcat
    18601 cgattgagcg aatatcctga ttttgcccgg tttcaaaaac accgtcttcc cccacccaca
    18661 gtgaagaaac ataaggaata atattctccg cttcaccttt tagcgttccg tcttccacac
    18721 gtaccgcaga cgtcgcagtg ccgctggcgg tcaactccag cgtcccgccg ccacttttag
    18781 tcaacgtgct tacgctatcc agttgttgcc cggtactatc cgccatcaac ccgccccatt
    18841 gcgtatctac gccatcctca acgtgtattt cgccatcagc gcgcatttca atattcctgc
    18901 cctgacctgt tgtagccgag cgagtgtcaa catcagtcgt cacatccatt tcactgtgtt
    18961 gttggggatc tgtgaagatc actgaacggt tgtaacttgt gtcccccaat tgcgaattat
    19021 cgctgacttc aagcgtaccg gaggcgattt gcgtattacc gagataaccg ttgttattcg
    19081 ccagcgttac gcgctgatcc cccgcattaa tgacgacata aacgccattc tggctggcat
    19141 cactgccgcc aatatgtaaa tcatcgccac tgctgatatc aacgacatca ttactgccaa
    19201 tcactgtccc cccgctgccg ctgatttgca gaccgtcatt gtatgagcta tcgccatccc
    19261 aggtggtgaa atcagagaga tccaatactc cgccatttag cacgatcgat tgcggatcgc
    19321 tctgcattgc ccgtaaatcg gcctgattcc ccgccagcga caatacggcg ttatcttcca
    19381 ccacaatatc gccggttaat gccatcgact gcgcccctgt caggagatag ctgccatctt
    19441 gcgctaccgt caactgtcct tcaccctgaa tcgagccgga aaaaccgccc tggtttacgg
    19501 taacattgcc gcctgcgtcg atattcaaaa tgccattagc aaaaccggtt aatgagtgca
    19561 caaacgtttg ttgtgtattg ccaacattca gttctgcctg atactcagaa tgaacagtgc
    19621 tgccgaccat cagaccaaag caatcctgcg gatcgctctg acagtgcgtg tcgccgacat
    19681 tcattaactc atcgctgcgc cccaacgtca cctcgccatt ctggatactc atctcgccgg
    19741 taaacgcatt attgtcggca ttgaggacta actctcccgc tccctctttc acgatgactc
    19801 ccgttccggc aagagaatca atcgcgccat cgttgctggt attgcctatt accagcgttt
    19861 tatcggcggc aatgttaaat tcagccacac tatgaccgat atacataaag ccccccgccg
    19921 ctgccgtcgc cgaatggtca tatcccgagg cgctgttatt ttcatcaatc atcacaccat
    19981 tgttttggtc ataattatca tcaacagaaa tatcaataag atacggggat gtggtgctgc
    20041 tggtataaat tgctccgcca taaccttctg catagttatt ggtaaaggag gtattattaa
    20101 ttatcgtttt tcctgaaaga tgtgtcttat ccgtactatt atccgtaaca tcaatggcgc
    20161 cgccatcccc atcactataa cttgttgatg tatatgcctg gttattatta aaagcatcat
    20221 cacttaaata gacgtcatta ttgatggtat aaattgcacc gcctttgccg tcattagcga
    20281 tgttattgtg aaatacggcg ttagtgactc gtaaatcggc ggcaccggta tcattggtac
    20341 cggcagaata gattgctccg ccatagcctc ctgcaacatt actgtcgaaa ataacattcg
    20401 caagatttat cgttgagttt tctttagcaa agatagcccc gccattatta tattcgccac
    20461 taaccgagtt atcagcaaac agggtcatcc ctgtttcatt ttgtggtgat atcgtaaatt
    20521 cagcactgtg ctgcaaaaat actgcgccac cgctggcatt attgaccata cccgaaaaaa
    20581 tcaaccattg tccatccgcg atgctccagt cctgtatatt acccgaaagg ctttgcctgc
    20641 tggcctgaca gctcgctttg atatcatatc cctggcatga atcaatgacc gctgcggcac
    20701 cgttagcaga gaaaagagat gcaatcattg acgggagtaa agataaatac tccttgcgta
    20761 gagagataat ccgcatattc atctaaccat cattttctat aagatggcgt atttaatcgc
    20821 attatgcgat atagggtatt tttcttttgt aaattctgcc cccgatgtaa taaccacttt
    20881 g
//
LOCUS       DAPQZD010000077        20478 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPQZD010000077 DAPQZD010000000
VERSION     DAPQZD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.077"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..387
                     /locus_tag="RO454_002744"
                     /pseudo
     CDS             1..387
                     /locus_tag="RO454_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525915.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(453..743)
                     /gene="ilvN"
                     /locus_tag="RO454_002745"
     CDS             complement(453..743)
                     /gene="ilvN"
                     /locus_tag="RO454_002745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDY0934557.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(747..2435)
                     /gene="ilvB"
                     /locus_tag="RO454_002746"
     CDS             complement(747..2435)
                     /gene="ilvB"
                     /locus_tag="RO454_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDY0934558.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(2541..2639)
                     /gene="ivbL"
                     /locus_tag="RO454_002747"
     CDS             complement(2541..2639)
                     /gene="ivbL"
                     /locus_tag="RO454_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDY0934559.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3040..4224
                     /gene="emrD"
                     /locus_tag="RO454_002748"
     CDS             3040..4224
                     /gene="emrD"
                     /locus_tag="RO454_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDY0934560.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4232..4729)
                     /locus_tag="RO454_002749"
     CDS             complement(4232..4729)
                     /locus_tag="RO454_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDY0934561.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(4726..5088)
                     /locus_tag="RO454_002750"
     CDS             complement(4726..5088)
                     /locus_tag="RO454_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDY0934562.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(5078..5425)
                     /locus_tag="RO454_002751"
     CDS             complement(5078..5425)
                     /locus_tag="RO454_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDY0934563.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(5485..6979)
                     /locus_tag="RO454_002752"
                     /pseudo
     CDS             complement(5485..6979)
                     /locus_tag="RO454_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(6976..8691)
                     /locus_tag="RO454_002753"
     CDS             complement(6976..8691)
                     /locus_tag="RO454_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDY0934564.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            8858..9724
                     /locus_tag="RO454_002754"
     CDS             8858..9724
                     /locus_tag="RO454_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDY0934565.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(9814..11475)
                     /locus_tag="RO454_002755"
     CDS             complement(9814..11475)
                     /locus_tag="RO454_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDY0934566.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(11673..12101)
                     /gene="ibpB"
                     /locus_tag="RO454_002756"
     CDS             complement(11673..12101)
                     /gene="ibpB"
                     /locus_tag="RO454_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDY0934567.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(12213..12626)
                     /gene="ibpA"
                     /locus_tag="RO454_002757"
     CDS             complement(12213..12626)
                     /gene="ibpA"
                     /locus_tag="RO454_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDY0934568.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(12620..12692)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            12932..13264
                     /locus_tag="RO454_002758"
     CDS             12932..13264
                     /locus_tag="RO454_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDY0934569.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(13268..14482)
                     /locus_tag="RO454_002759"
     CDS             complement(13268..14482)
                     /locus_tag="RO454_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDY0934570.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            14583..15683
                     /gene="cbrA"
                     /locus_tag="RO454_002760"
     CDS             14583..15683
                     /gene="cbrA"
                     /locus_tag="RO454_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDY0934571.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(15644..16981)
                     /locus_tag="RO454_002761"
     CDS             complement(15644..16981)
                     /locus_tag="RO454_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934572.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17056..18204)
                     /gene="dgoD"
                     /locus_tag="RO454_002762"
     CDS             complement(17056..18204)
                     /gene="dgoD"
                     /locus_tag="RO454_002762"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDY0934573.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(18201..18818)
                     /gene="dgoA"
                     /locus_tag="RO454_002763"
     CDS             complement(18201..18818)
                     /gene="dgoA"
                     /locus_tag="RO454_002763"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDY0934574.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(18802..19680)
                     /gene="dgoK"
                     /locus_tag="RO454_002764"
     CDS             complement(18802..19680)
                     /gene="dgoK"
                     /locus_tag="RO454_002764"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDY0934575.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            complement(19677..20366)
                     /gene="dgoR"
                     /locus_tag="RO454_002765"
     CDS             complement(19677..20366)
                     /gene="dgoR"
                     /locus_tag="RO454_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDY0934576.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
BASE COUNT         5076 a         5469 c         5144 g         4789 t
ORIGIN      
        1 tataccttct ggtagcacta cccattctac aaacatacta agcctgaata taaagagtac
       61 gactgggaac aaattcaagg tatagctttt tttgcgcaaa atcaccattc cacatacgcc
      121 accattcgct gtattgttta tgaatcatta gacagtacaa atattattga cacctttaac
      181 ctgataacga cagagagtgc tacaaatgca ttgcatatga caaataacaa attatggtta
      241 tggattaata gctttatgaa ttttgacgat gagtttttag agaaatctat caacactgct
      301 ccaggtattt ggggccgtga agtgaattgg cctgatgata tgaataagaa atcaatgttg
      361 tcttcaatgg acgaatatat actctgaata atttgagttg cagaatataa gctcgcccgg
      421 atgtaaatat cccatccagg cgagaaaatc acttactgaa aaaacaccgc aatcttgtta
      481 aacatcgtcg ggtcggactg attacgctgc actttcacca catcttccag cttgtcgatt
      541 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat gtggcttttg
      601 tcgctgtcct gaatcggcag gcagagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
      661 aggccacaaa cgtgggtcat tacacccgga tgattgcgaa cggtgagctc cagaatcacg
      721 ttatcatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
      781 ggcggcacca tcgggtacac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
      841 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
      901 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggta
      961 gcggcaaaaa cgccctgctt gtagaacaga ctctgttgct gatgcaccag ccccagcgct
     1021 tcgttgttca tcaaaatgat tttaacatcc agctgatttt cactggcggt cgccatctcc
     1081 tgaatattca tcatcaggct gccgtcgccg gagaagcaca acactttgcg gtccgggttc
     1141 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccgccggag
     1201 gtcagccact ggcgtgggcg attgagcgga taggcttgcg cggtccacat ctgatgctga
     1261 cccacgtcgg tagtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
     1321 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc acgctgcaaa
     1381 tccgccacca actggtgcca ctctgcacgc ggttgcgctt ccaccagcgg gatcaactgc
     1441 gccagcacgt catcaacatc cgcctgaatt gccacatgcg gttgcttgat tttgcccagc
     1501 tctgcgcggt cgatatcgac atgaataatt ttggcattcg gacagaactg ctctgttttg
     1561 ccaatcgccc ggtcatcaaa acgcgcaccg agtacaatca gcagatccgc ctcctgcaaa
     1621 atatagttgg tgctgcgcac accgtgcatc cccagcatac ccagcgacag cggatgcgct
     1681 tttggcaaca tacccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
     1741 agttcacgca ctcgcgcagg cgcgttgatc acaccaccac ccagataaag caccgggtgt
     1801 ttggcggcgt taatcatcgc cgccgcgtca cgaatacttt cttcgctaaa ggcgggtgcg
     1861 gcggcttttt ctgccatagc gggctgtgtt tcaatctcaa aaactgccgt ttgcacatcc
     1921 ttaggaatgt ctatccacac cgggccaggg cggcctgatt gcgcaatgct gaaggcatcg
     1981 ctcatgacct gcgggagttc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
     2041 atagagatgc cgtaggtgtc gacttcctgg aaggcgtcgg tgccgatcat cgaggcggaa
     2101 acctgaccag tgatgcaaat cagcgggatg gagtccagcc tcgcatcggc aatggcggtc
     2161 accaggttag tcgcacccgg tccgctacag gccatacaga ccgccggttt accgtcggtg
     2221 cgcgccattc cctgggcgat aaaccccgcc ccttgttcgt ggcgagccag aatatggcgg
     2281 atttgcgtgc tttggcttaa ggcatcgtaa acaggcagga tagaaccgcc cggaataccc
     2341 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
     2401 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
     2461 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
     2521 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
     2581 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
     2641 agggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
     2701 caatggaaaa tttttcttcc ggataaaaat acgtggcaga tcacattttg aactgaaact
     2761 caggcaataa agcatcatca cctgaaagac ggtcaggttg ttgacagagt gcgccttatt
     2821 catgtcgaat acagcgtaaa cactttactc taccaaccgc accaccactg tttccggcaa
     2881 catttattgc cgcttttgtt tacatattct gccgctaaac aattccccat tcctggcgta
     2941 tatctggcta acattcatca atgtgataga ttcctctccc gcatttatgg gaatgcgtag
     3001 tgacttattc taattatttt tataaaagca tccgtgataa tgaaaaggca tagaaacgtc
     3061 aatttgttat tgatgttggt attactcgtg gccgtcggtc agatggcgca aaccatttat
     3121 attccagcta ttgccgatat ggcgcgcgat ctcaatgtcc gtgaaggggc ggtgcagagc
     3181 gtaatgggcg cttatctgct gacttacggt gtctcacagc tgttttatgg cccgatttcc
     3241 gaccgcgtgg gtcgccgacc agtgatcctc gtcggaatgt ccatttttat gctggcaacg
     3301 ctggtcgcgg tctcgacctc cagtttgatg gtattgattg ccgccagcgc gatgcagggg
     3361 atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc cgcgcgattt atatgaacgg
     3421 acacagttgc gccacgctaa cagcctgtta aacatgggga ttcttgtcag tccgctgctc
     3481 gcaccgctaa tcggtggtct gctggatacg atgtggaact ggcgcgcctg ttatctcttt
     3541 ttgttggttc tttgtgccgg tgtgactttc agtatggccc gctggatgcc ggaaacgcgt
     3601 ccggtcgacg caccgcgcac gcgcctgctt accagttata aaacgctttt cggtaacagc
     3661 ggttttaact gctatttgct gatgctgatt ggcggtctgg ccggaattgc cgcctttgaa
     3721 gcctgctccg gcgtgctgat gggcgcggtg ttagggctga gcagtatgac ggtcagtatt
     3781 ttgtttattc tgccgattcc ggcggcattt tttggcgcat ggtttgccgg acgtcccaat
     3841 aaacgcttct caacgttgat gtggcagtcg gttatctgtt gcctgctggc tggcttgctg
     3901 atgtggatcc ccgactggtt tggcgtgatg aatgtctgga cgctgctcat tcccgccgcg
     3961 ctgttctttt tcggtgccgg gatgctgttt ccactggcta ccagcggcgc gatggagccg
     4021 ttccccttcc tggcgggcac cgctggcgcg ctggtcggcg gtctgcaaaa cattggttcc
     4081 ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag cctgggattg
     4141 ttgatgacct taatgggatt gttgatcgtg ctgtgctggc taccgctggc gacgcggatg
     4201 tcgcatcagg ggcagcctgt ttaagctcac gtcaccgcag catcgtcatc agctccatgg
     4261 gagaacgatg ctgctttatc agatcacgca tcacccgcat atgcggcgcg gagtaagaat
     4321 aaaacgcctg atagcccgca caaagcacgt tgtctggttt atcttcccga gcgtgcgggc
     4381 acaggcgatg ccagtcgcac gcctggcagt cacggcgcgg ttgcgacttt tcgctgccac
     4441 accatttttg taaggtggtg tcaaaaagtt caatcgaaat acgactgata tctgcccgca
     4501 cccaacactc gaacagggca attaaaaatt tcccccacag ctctgctgtc accgaagcgg
     4561 ccgtcagatc gccccggtca tcaaattcaa gatggggaat aagctgtact gagcggatgc
     4621 ccagctgttt gaaattgtga taaaaatcgg caggttgcag agcatcctta tactctacaa
     4681 ccagtttatg cctgtcctct tcagcatcaa tgacctgact tcctgtcatg cgactctctc
     4741 aataaatacg ataagttgat gtatatgcgt tacagcaaac agtattgcga gagataacac
     4801 cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt
     4861 gacatccatt aaattgcgat tacgggtgta gtgccagagg atcagcgcca caatggcgag
     4921 gatgccaatc gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa
     4981 cgccatcaac gcgccgtagc ccagcatggt acgaaaccac gccagtgatg tgcgttccgg
     5041 ctgcagccct gggtcggcaa tgcgtctggc tttgcggcta tccggcatac aacaccagtc
     5101 ccatgacgat cactgccacg accatcaata ttaagctgat gatgaataag ctgttggtat
     5161 agggcaggtc ctctttcagg cgcatcgctt tttcattgcg taaccagcgc aagtagccat
     5221 acatcgctaa cccgccggaa aacagacaca acaacaacgc cagcagttcg cggatgacgg
     5281 gcgtggcgaa atctggcgca agttgatcaa gcccaacccc cgccgccaga aatcctagtg
     5341 cagtacggat ccacgccaga aaagtacgct cattagccag cgagaagcgg taatcgggcg
     5401 cttctccgag gcgggaaatt ttcattgttg ctccttgtaa actgttcctc gccgaaaaat
     5461 taggccagga acagcaataa tgcatcagaa cttctgtttt ttctcttcca ccttcacccc
     5521 ttgtgtcggt aaaccggtgt catagtcgcg taccactgga gaataaccat cctgtgggcg
     5581 tggacgaaac gcccccatcc agcgcggttg tgcatctttg cgccacggac gcaggttcca
     5641 ttgataactg cggaacggat cgcgaatttt gtccatgtaa tccaataagg cgtcatgcat
     5701 tttgctgcga acgtcggcaa aatggatatc atcgatcagg ttatgcattt catttgggtc
     5761 attacggcga tcgtaaagtt catcactggt gaagaggttg agcaccagtt taaagtcatc
     5821 cgttacccag caacgcaccg gaataaaacc gccaaagctg tcatgctcaa tctcgtagcg
     5881 attaaattcc accaacacgc cacgtggttc ttttacggca aggatatttt cccccggcag
     5941 aatctctggt ttttcaatat ctgccagcgc catcattgtc ggcagtaagt caatatgact
     6001 gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa cggatgatca gcggaatgcg
     6061 ggtgatgtcg tcatacatcg ccgctccttt actgatcagc ttatgtgcgc ccatcatttc
     6121 gccgtgatcg gaggtataaa taacccacgt attttcacgt tgctctggcg ttaaggcgtt
     6181 gatgacccgt ccgatttggt catcaacaaa gtcattgcag gcaaaataga gcggatggtg
     6241 ataaagcccg tcatcaccga ctggcgatgg catcgcctgc gcccataagc ggtgatgttc
     6301 cggtttgttc gccaggtcat cctctgcttt ctcacccagt tcgtagtaaa aatcagtgta
     6361 tttctctaaa tactccaccg gacaggtgaa cgggtgatgc ggctcatcat acgaaatcac
     6421 catcaggaaa ggttcgtcgg cgcgcgcggg ctgttgcaga aaatccaccg cccgattgct
     6481 gatgcggtgc gcccaggtga aggtttcgtc aatatggttc gcctgtaaat cttcgacgct
     6541 gtttaggcca ttgcgccaca ggctaatctc tttttccgtc agttcactaa gatagttcgc
     6601 cccatcgaac cagtaatcag cgtcccactc cggcggacac tcgccaggtg ccgaaatagt
     6661 catgaccgtc gagatgccat ttgccgatgt aacaggtgtg atagccggca tccttaaagt
     6721 agcgccccat agtggagatg tttttgcctg gcgcgacgtt gttggtccac gggccggact
     6781 ggttagcgta gataccggtg aatagtccgg cgcgtgcagg cgtacaaacc ggtgaacagg
     6841 tgtaggcgga gttaaagcga ataccttccg ccgccagact atcaatattt tgcgtattca
     6901 gcggcttacc gctatagcaa ccgaccatat tggtggcctg ggtatcggtc atgatgaaca
     6961 gaaaattggg gcgtttcata ggctatcctt tgcatcggga gtaaaggtta cggctgggtc
     7021 gtgacgatgc cgccagctgt caaaaatcag gtagacaatc actacggcgg caatgaaata
     7081 actgatcatc gctaaccagc gcgtaccgta gccgccgaat tcagccagcc cggcatagac
     7141 gccaatcatc gcgaacagga taccaattga cgcagtcttg acgtttttcc acggtttcat
     7201 gtccaccgca aacgcatctt tgaaggtgaa cggcgtggcg cgcggtttga taaaaccgat
     7261 caccagcatc acgaccacgt tgatgcagaa cgtacaggcc agcacataga ggaaatggaa
     7321 gtcgaacttc accagatagt tgatggtgat gtagctgatt atgccaatcc ccatcgccac
     7381 ttttgccgcc agcgccggaa tgcgcgggaa gaaaaagccc ataatgatga tggtaaccag
     7441 cggcacgttg taaataccgt tgagctgttt catccagcta tacagcccct gcggcgcgtt
     7501 ggcgatccac ggcgcgacca tcaccgaaac gatggcgata aagaaaccaa atttgcgccc
     7561 gacggtgacc agctgttgcg gctcggcatt ctggttaatg atacgccggt aaatacccat
     7621 actgaataac gtactggcgc tattcagaaa gccgttgaag gtactgatca ccgcaccaaa
     7681 taacaccgca ccgaagaagc ccaccagtgg caccggcaga acgttattga ccagcgtcgg
     7741 gtaggccatg tcggctttcg gtaaatcctg atacagatga aacgcaatca accccggcag
     7801 caccagtacc agcgggtcga gcattttcag caccgccgtt aacagtgccc ccttttgccc
     7861 ttccgccagg ctttttgacg ccagcgtgcg ttgcacgatg ccctgattgg tacaccagta
     7921 aaaggtgtta accagaatca aaccggtaaa tgccgcgcca atcggcaagg gatcggtcgg
     7981 gccaccgact gagtttaatt tctcggcgtg aacggtggta agttgctcaa tgccctgcat
     8041 aaagctgccc ttgcccatcg cgatcaggcc aaataccggc accatcaacc cgccaataac
     8101 cagcccaata ccattgatgg agtccgccac tgccattgcg cgcagtccgc cgatcaccgc
     8161 atacaaaatt cccgccagac caagcaaaat aaccagtaac cagatagccg caccatcgga
     8221 aagttgtagc gactcaccca catgaaacag gctgttgagc gccaacgcgc cggaatagag
     8281 aacaatcggc agaaagcaga cgccggtggc aatgaggaag cagaagtcga tgataatacg
     8341 cgtcgtttta tcataacgtt cctccagaaa atcggggatg gtggcaatgc cgcgcttcag
     8401 atagcgcggt aaaaagatta gcgcgaggaa gatcagcgtc actgcagagg tgacttccca
     8461 gcccatcacc gacatgccgc ttttgtaggc ctgcccggaa aggccgacca gttgttccgt
     8521 ggaaaggttg gttagcatta acgaagcggc aataaccggc gcttttagtg aacgtccggc
     8581 aagaaaatag ccttgttgcg atccggtatc tgttttacgg accttccacc aggtgatgac
     8641 cgccaccagc agcgtaaaac cgacaaaact caagatttgt aacgaattca tctcatagcc
     8701 cttatttatg tttatgtccc ggacgcatga atatagggag aggtaaaatg cgtcgggtca
     8761 ggcagtcaga ttcttaaaat acaaacgtcg tatccctgaa cggattgttt tctgttaagt
     8821 tcaggttgtg agcataattc gccggaggga tattaaaatg aatggaaaat tgcaaagttc
     8881 ggatgtaaaa aacgaaactc cgtacaatat tcccttattg attaacgaaa atgtgatctc
     8941 cagcggaatt tctctgatat cgctctggca tacctacgcc gacgagcatt atcgggtgat
     9001 ctggccgcgg gacaagaaga aaccgcttat cgccaactca tgggttgcgg tgtataccgt
     9061 acaaggatgt gggaaaattc ttttaaagaa tgacgaacaa ataactctgc atggcaactg
     9121 tattatattt ttaaagccaa tggatattca ctcttatcac tgtgaaggtt tggtctggga
     9181 acagtactgg atggaattta cccccaccag tatgatggat attcccgtcg gtcagcaaag
     9241 cgttatttat aatggcgaag tttataatca ggaacttacc gaagttgctg agttaattac
     9301 ttcaccagaa gcaataaaaa ataatctggc agtcgctttt ctgacgaaaa ttatttatca
     9361 gtggatttgt cttatgcacg cagtcggtaa aaaagatcca caacggcggc aaattgaaaa
     9421 attaattgcc actttacatg ccagtctaca acaacgctgg agcgtagctg atatggctgc
     9481 cacgatcccc tgtagcgagg cctggttgcg tcgtctgttt ttacgctata ccggcaagac
     9541 gccgaaagaa tattacctcg atgcgcgtct ggatctggcg ctatcgctat taaagcaaca
     9601 agggaactcg gttggcgaag tcgctgatac gctcaacttc ttcgactcct ttcatttcag
     9661 caaagccttt aagcataaat ttggttatgc gccgtcagct gtgctgaaga atacggacag
     9721 gtaataacca gaatggcgag tgtgtaaatg actcgatgat gttcagaaat accaggcgga
     9781 gtaccattta cggcgactcc accgggaaaa gagttaaccg atactccaga agagcaccgc
     9841 cagtaattgg ggtgtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga
     9901 gagcgccgct gcaccgctgg ttggatgaag attattagca aacgccagcg ccggaggatc
     9961 ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg
    10021 cgccagaata ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca
    10081 gcttagccct tcgccatcca ccagtgtatg gataaaatcg ccgccggatt tcaggccaac
    10141 gaccgagagg aacagtacga tccccagctc ccgcagcgcg aggttggcgc ttggcggcat
    10201 aaaccagtac agcttgccga tactgccgat acgcccgaga atcaacgcca taatcagcgg
    10261 accgcccgcc agccccagtt tcaacgcggc cggaaatccc ggcacaaaga cgggaataga
    10321 acctaacaat acgccaagcc cgataccaat aaacactggc agcatctgaa cctgttgcag
    10381 tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac
    10441 caggttgagg atatcgccga actgcaggct gatatcgcta cttgcaacta attcaacacc
    10501 agcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat
    10561 acgtttaccg agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgcc
    10621 tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc
    10681 cgcaggctgc cccaccagat gcagcaaatc gcccagttga ataacggttt ctggcgaagg
    10741 cacttttagg gtttcttctc gtttcaatcg tgagcaaata actttatcgc cattgagaat
    10801 cggcacatct ttaatggcta aattatgcaa attaggattc tcaactcgga tgttaatcgt
    10861 cctgattagc gcaccgccgt tggttcgcgt ggactcgtgc tgctgggcct cagtctcaac
    10921 attgacgcgg aaaataaccc gcagcatcca catagtgaac aaaatgccgc aaatcccaaa
    10981 tggatacgcc atcgcatagc tcatccccat ttgatcgacc atcgccatcg gtgtccccag
    11041 gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggtgaccg cgccggaaaa
    11101 aatccccagc acgaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag
    11161 accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa
    11221 tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc
    11281 aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa
    11341 atggccgacg atgatcccgc cgaacagcac gccgccaatg cccaatccaa caccgcgaaa
    11401 cttgacgttg ccgataaaca acccgacgac cgccaccaaa gccagaatac tgaccgttaa
    11461 tgctatatca ctcatcgtcc atttctcttg aaataacatt ttaattgcta cggattatgg
    11521 ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag aacgtgccga
    11581 aatatcttaa acaggcgaaa tttaacgcat tgttgaagaa aaaagacaaa aaaaaagccc
    11641 caccagaatg gcggggcaaa gagaatagct agttagctat ttaacgcggg acgttcgctg
    11701 atagcgatac gctgcgctgc gatgggttca ggctcattac gaattaaatc aatatgcagt
    11761 aaaccgttta cgaaggttgc gccagagact tccatatttt cagccagcgt aaagctcagg
    11821 ctaaaaggct gattcataag cccttgatgc agccattttt tctcttcttt tggctgctcc
    11881 ggtgtgcctt ttacgctcag gcgcgtacct tccagttgaa tctctaaatc ttcctgacga
    11941 aaacctgcca gcgcgagggt aatgcggtag tggttatcgt cgcttttctc aatgttgtac
    12001 ggcgggaagc tctggctttc accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg
    12061 atccattgac gcatcagtgg ggataaatcg aagttacgca tagtcatttc tccttctaag
    12121 aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc tccctgacgg cgagcatgga
    12181 gatgtcaggc cgcgccaggc ggccttaggg aattagttga tttcgatacg gcgcggtttt
    12241 ttcgcttccg gaatcacgcg ttcgagatcg atatacagca gaccattcac caggttagca
    12301 ccacgaacat gaatgttctc agctaactgg aatttacgtt caaagttgcg ttcagcgatg
    12361 ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt caccaccagc
    12421 agattatcct gggcggtaat ttccagttcg ctctcagcaa aaccagccac agcgatagca
    12481 atgcggtaat ggttttcgtc taccagttca acgttatacg gagggtagcc gccattactc
    12541 tggctctggt tgttttctaa gtggttaaac aaacggtcaa atccaatagc agaacggtaa
    12601 agcggggata aatcaaagtt acgcataatc aatagctcct gaaatcagcg agaatctaag
    12661 accttccaca atggacaggt caggtagcca gaacacccat caggcgcgtc ctcatcggct
    12721 acgatgtaaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga cttttttttg
    12781 cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag tgcgatagcc
    12841 gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata actcatcagg
    12901 ccaacaccca cgatcaacag atgaatttat tatgataaaa aatgtgttgt tagcgttcat
    12961 gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc ataccggggg
    13021 gaaagaagga acgtatcctg gcacccgcgc cagcgcaacc atgattggcg acgatgaaac
    13081 caactggggc acaaaatcgc tggcgatact cgacatgcca tttaccgcag tactggacac
    13141 aatattgcta ccgtgggatg tgttccgcaa agacagctca gtgcgctcgc gcgttgaaaa
    13201 aagcgaagcc gacgcgcaag aaaccaataa cgtcattccc cccgccaaaa tgcctgcaaa
    13261 ctgagagcta gcggtcagtt tcggtgatcc acaactgacc gtcctccatc cacgccaacc
    13321 attgaccatc cggcgagaaa accacggcgt ccgcagaagg tggattggcg tggttttcgg
    13381 ttaaatactc aacctcgccg ctttgcgcat gggcacaagc aatccgatta tccagcacaa
    13441 atcccagcca ttcgcctgat ggatgccagt taaatgcaga ctgaatatcc gttttattat
    13501 gggttaactg gcgtggttca ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    13561 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    13621 gcgggacgtt gactaatccc ggagaagccc gatggtgcgt aaaggttaaa cgtcgctgca
    13681 cgacaccacg cggtggcgcg ggcagggttg tttccgttcc acttaacggc gcatcacctg
    13741 ccactttcca gccagcttca tcttgcggca actcgacaat aaacagctcc ggcactttct
    13801 cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt
    13861 cttcataagc acgattgatt tcatcactgc caggctgtgg cgtgggcgtg gttttactta
    13921 ccagcacgca ccagtggcta ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg
    13981 ggccaaacgg tgcagcaaca ccgacatttc gcaaatccag cgccggatcg agttcatgca
    14041 ttacatggtc gttgtaggta aagctcaccc tttcaccgtt tgggctaaag acatgtacat
    14101 gactgccgcc gcgcagcgct cctggcgtat atggcgcggt aatatccatt gcatcgagat
    14161 tgctcacctt gccgctttca acaatcactc cgcgccgatg atgaaagtcg taataccatg
    14221 tttcatcagg attttccggg ccgtggatga agacatactt ctctgacttc ggatgaacgg
    14281 tcaccacgcc gacgtatgcg ccctgtgacg cgcgatagat aacctcgacc tcgccggtat
    14341 ggatattcac acgctcaatg gtctcgccgg taaacgacgc cccagaaggc cgcacgtcaa
    14401 acaccagcca ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg tgattacggg
    14461 gagcaaaggt tatctgtttc atggcgcggt cctgatacaa aacggttccc gccatcatac
    14521 ttcacaagga cttcactttc agacaatagt cttttgccat cgtgatgaat gtgagtgaaa
    14581 agatggaaca tttcgacgtg gcgattattg gcctcggccc ggcagggtcg gcgttggcgc
    14641 gaaagttagc aggcaaaatg caggtgatcg cgctggataa aaagcaccag tgtggtactg
    14701 aaggtttcag caaaccttgt ggcggtcttc tggcaccgga cgcgcagcga tcttttattc
    14761 gcgatggact gacgcttcct gtcgatgtca tcgccaaccc gcagattttc agcgtcaaaa
    14821 ccgtcgacgt cgccgcatcg ctcacgcgta actaccagcg aagctatatc aatattaatc
    14881 gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag gtttaccacg
    14941 acagcctgtg tcgaaaaatc tggcgtgagg atgataaatg gcatgtcatt tttcgcgcag
    15001 acggctggga gcagcatatt accgcccgct atctggtcgg tgccgatggt gccaactcga
    15061 tggtgcggcg acatctctac ccggatcatc agattcgtaa atatgtcgcc atccagcagt
    15121 ggttcgcaga gaaacatccg gtaccgttct actcctgcat ctttgataat gcgataactg
    15181 actgttactc atggagtatc agcaaagacg gttattttat ctttggcggt gcctatccca
    15241 tgaaagacgg tcagacgcgt ttcacgacgc taaaagagaa aatgagcgcc tttcagttcc
    15301 agtttggtaa ggcggtgaaa agcgaaaaat gcacggtgct gtttccctca cgctggcagg
    15361 attttgtctg cggcaaggac aacgcctttc tgattggcga agcggcggga tttatcagcg
    15421 ccagctcgct ggaggggatt agctatgcgc tggatagcgc agagattctg cgttcggtat
    15481 tactgaagca gccagagaag ctcaataccg cttactggcg cgccactcgc aaactgcgtt
    15541 taaaactctt cggcaagata gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt
    15601 ggattatgcg cagtggagtg gcacatattc cacagttgaa agattatcca acgcgcttca
    15661 catcgcccac cagcaggatg taagagagcg cgccaatcaa cgcgacggcg gagatataaa
    15721 ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaccc caccaccagc ggaacggtga
    15781 tgccgcccag accaccggca aagttaaaca cgccgccggt taaaccaatc aggcgcatcg
    15841 gtgccagaga agagaccagc gaccaggtta tcgaagcaaa accgttgccg aagaatgcca
    15901 gcgccatcag gcacataatc atcatcggat cgttggtgta gttcgcaccc ataatgcagg
    15961 tggagatcag caagccgcag ataatcggcg ttttacgcgc aaagcccagt gaaaagccct
    16021 tacgtaccag cagatccgcg acccagccag agagcaggac gccaataaac gccgcgagga
    16081 atggcaccgt ggtcataaaa cctgctttca gcgccgtgat tcctttttcc tgggttaaat
    16141 agttcgggaa ccaggttaag aaaaaccaca gtgtagaagc caccgcaaat tgcccaagat
    16201 agacaccgat cagtttacga tggaacacca gtttccagtc tttggctgtt aacggctgac
    16261 gcgcctcttt tttcaccggc gcatcgccat ccaccagacc gccgccatca cgaatgtaat
    16321 ccagttcagc tttgctgatg cctttggtca gacgcggcgg ctgataaacc ttaaaccaaa
    16381 tcagcgacca gataatgccg ataccaccgg tgacaatgaa cacccagtgc cagctcaaca
    16441 tctcctgaat ccagatcagc agcggagtca gaaacgccag accgacaaac tgaccagacg
    16501 tataaaaacc aacggcagaa gcgcgttcat gttccgggaa ccaactggtt accatccggt
    16561 tattggtcgg gaacgcgggc gcttcgaaaa taccggttat cgcgcgcaga ccaattaatg
    16621 acattaagcc cgtggcaaag ccctggaata acgtcgccac tgaccagcca aatatcgcaa
    16681 taaaataagt cacgcgtgaa cctacgcgat ctaaaaacca accgccgggg atctgacata
    16741 gcgtataaag ccaggcgaag gccgaaaata catagcccat ttgcgcttta gtaataccaa
    16801 actcttcctg aatatgggca gaagccacgg ccaggttagc acggtcaaca taacaaatga
    16861 ccaccgtaat aaagatcatc accagcgtca gataacgccg acgccccggc tttgctgcat
    16921 taacgggaat atccatagcg agctttctcc tgattttggg catagcgaag ccgctcacca
    16981 tgcactgtaa tttacagagg gttatatttt tgtattgctg ttttagtgcc cgatgagggg
    17041 cttacgtggc aggaattacc actctgctac gctgttatct tcatgacgcc agagcggatt
    17101 acgccagtcc ggggcatttt tactgaactc aataacttta gcttcgtcga tttccacgcc
    17161 taagcccggt ttcgttaatg gtttaaagaa accgccgacc atgctgaagt cctctttgtt
    17221 tttcacaaag tcgagtaact ccgcgccttt gttgtaatga atgcccatac tttgttcctg
    17281 aaggacggcg ttataggaaa caaagtcgat atgcaggcaa gccgccagtg caatcggtcc
    17341 gagcggacag tgcggcgcaa gggtcacgtc ataggcttct gccattccgg caattttgta
    17401 gcattcggta ataccgcctg cgtgggagag atccggttgc agaatcgaaa taccgcctgc
    17461 ctccagcaca cgtttaaaat cgaagcgtga gaacatgcgc tcacccgccg ccagtggaat
    17521 atgcgtttgt gccgccagtt tcgggtagta ttcggcctgt tccgccagca ccggctcttc
    17581 aataaacagc ggacgatacg gttccagctc tttaatcagc actttcgcca tcggcgcgct
    17641 gacgcgacca tggaagtcca gaccaaactc aatctgattg ccaaaagctt cacgaatttg
    17701 tgccacggtg ttgactgccg catctaccgc gcgggagtta tcaattagcc ccagttcttc
    17761 acaaccgttc agtttgaagg tatcgaagcc gatttcgcgt agcgttttaa tgccgtcgat
    17821 aacatccgcc ggacgatcgc cgccgaccca actgtaggct ttaattttgt cgcgaaccag
    17881 gccgcccatc agttgccaga ccggcgcatt cagcactttg cctttgatat cccataaagc
    17941 ctggtcgatc ccggcgatgg cactcatcag aatcggaccg ccacgataaa atccggcgcg
    18001 atacatcact tgccataagt cgttgatgcg tgacgggtcc tgaccaatca aatagtcact
    18061 cagctcgtgg actgccgctt ccaccgtgcg agcgcgacct tcaatcacgg gctcgcccca
    18121 accgaccacg ccttcatcgg tttcaatttt caggaacatc cagcggggag gtaaacgata
    18181 cgtggtaatt ttggtgattt tcattgcact gcctctcgat acgccttaac aaatgctgct
    18241 gcctgctgcg cagtccgttc tacggactgc ccggcgcgat agagatcgct acctaagccc
    18301 gctccggcac agcctgcatc tatccactgc gccaggtttt ctggcgtcac accgccaacg
    18361 gcaaagaccg cgatgttcgg tggtaatacc gcttttaatg ctttgatgta ttgcggacca
    18421 aaagccgatg acggaaatat tttcagtgcc tgcgcacccg cgtcgagcgc ggtaaaggct
    18481 tcggtcgccg tcgcgcagcc ggggcagacg gtcatgccgt agcccaccgc acggcggatc
    18541 acttcactat ggatattggg cgtaacgatg agctgacagc ccattctggc gagcgcatcg
    18601 acctgttcag gtttcagtac cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca
    18661 acgatggcgg gaatgctctg ctcccattgt ggagaattca gcgggatttc aaccgcgtcg
    18721 aacccggcgt caatcaccgc gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc
    18781 aaaatggcga tcagcgggag tttagtttgc cactgcatga gcgatgttcc ttataccagc
    18841 ctgaaatgcc gtgtcgcccg ccaccgccgt cacgtcacaa cccatcgcct gaaaggcttg
    18901 ctggtagcgc gcggtcagcg atgttccggc gacaagggtg atgacttgtt gatgggccac
    18961 atagtcgcgc atactggcga cctctgcgcc aatcaacaaa ccagagagaa attcgctgac
    19021 ctgttcgcgg ggaagtgttc ccagcacatg cgaggcgcga acttcaaaaa gctgtggcaa
    19081 tacggcgggc gcattaaggc cacgctcaag accagccgcg aaagcatcga cagcgttttc
    19141 ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat gtaattcacc
    19201 ggtcatcacg gtgcgaaaat cgttaatttg ctggctatcg gcctgcaccc atttgcaatg
    19261 cgtaccgggc atgacataaa gagaggaagg agcgagagtg cgcgcgccga tcaattgtgt
    19321 ttcttcgcca cgcatcacat tgtggttatc gtcatgagag acgcataatc cgggaataat
    19381 ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaac gggcaggaat
    19441 ggataaatac ggcgcaactt tccagccgac attgctgccg accattcctg ccattaccac
    19501 tggcgtattc tcttcacgcc agtcggtcgt cacttctgct aacaccgcag ccggagattt
    19561 tccattcagg cgcgtgacgc ctgcttctga ttgcctgctg tccaggcagt ggtcgccctg
    19621 ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat
    19681 gtgatttcct ttaaccttcg tgtcgagctg gcgatcatgg taagcgccgc ctgctctgcc
    19741 gcatcgccgt cctgatgcct tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc
    19801 ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg
    19861 atcgcaatgc tcagttgttg taacaccggg ttatgcaccg actgcagcac cgcctcgtgg
    19921 tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttagcaat catctcgttc
    19981 agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca
    20041 atagccggct ctaccaggtt tcgcacttcg ctcattgcac tgataagccg tgggtcatag
    20101 tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg gttacgcgga
    20161 gccacaaacg ccccgcgata acgtttcatt tcaatcagcc gcttcgccat cagcgaacga
    20221 aatacctcac ggatgatgtt gcgcgaggtt gcaaactcct cacagagttc tgcctcagcc
    20281 ggaagcggcg agcctggcac gtatttgccg tgaacgatct gtttacccag cgtaataacg
    20341 atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt tcctctctac
    20401 tttaccccga tcgatgcata acgcggcaat tttgtagtac cagcgtgatg acgttcgcgt
    20461 ttgctgtgcg cataatgt
//
LOCUS       DAPQZD010000078        20470 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPQZD010000078 DAPQZD010000000
VERSION     DAPQZD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.078"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            171..323
                     /gene="hokA"
                     /locus_tag="RO454_002766"
     CDS             171..323
                     /gene="hokA"
                     /locus_tag="RO454_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDY0934577.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFL
                     ACKLKE"
     gene            complement(511..723)
                     /gene="cspA"
                     /locus_tag="RO454_002767"
     CDS             complement(511..723)
                     /gene="cspA"
                     /locus_tag="RO454_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDY0934578.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1004..1294)
                     /locus_tag="RO454_002768"
     CDS             complement(1004..1294)
                     /locus_tag="RO454_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDY0934579.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1728..2438
                     /locus_tag="RO454_002769"
     CDS             1728..2438
                     /locus_tag="RO454_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDY0934580.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2488..3462)
                     /gene="ghrB"
                     /locus_tag="RO454_002770"
     CDS             complement(2488..3462)
                     /gene="ghrB"
                     /locus_tag="RO454_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDY0934581.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVVD"
     gene            complement(3566..4225)
                     /locus_tag="RO454_002771"
     CDS             complement(3566..4225)
                     /locus_tag="RO454_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDY0934582.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4378..6711
                     /gene="bisC"
                     /locus_tag="RO454_002772"
     CDS             4378..6711
                     /gene="bisC"
                     /locus_tag="RO454_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDY0934583.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(6680..7120)
                     /locus_tag="RO454_002773"
     CDS             complement(6680..7120)
                     /locus_tag="RO454_002773"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0934584.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7117..7680)
                     /gene="tag"
                     /locus_tag="RO454_002774"
     CDS             complement(7117..7680)
                     /gene="tag"
                     /locus_tag="RO454_002774"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDY0934585.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7838..8536
                     /locus_tag="RO454_002775"
     CDS             7838..8536
                     /locus_tag="RO454_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0934586.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8765..9973
                     /locus_tag="RO454_002776"
     CDS             8765..9973
                     /locus_tag="RO454_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934587.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10297..11988
                     /gene="eptB"
                     /locus_tag="RO454_002777"
     CDS             10297..11988
                     /gene="eptB"
                     /locus_tag="RO454_002777"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDY0934588.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12080..12156
                     /locus_tag="RO454_002778"
     tRNA            12080..12156
                     /locus_tag="RO454_002778"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12114..12116,aa:Pro,seq:cgg)
     gene            12900..14507
                     /gene="dppA"
                     /locus_tag="RO454_002779"
     CDS             12900..14507
                     /gene="dppA"
                     /locus_tag="RO454_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDY0934589.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14814..15833
                     /gene="dppB"
                     /locus_tag="RO454_002780"
     CDS             14814..15833
                     /gene="dppB"
                     /locus_tag="RO454_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDY0934590.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15843..16745
                     /gene="dppC"
                     /locus_tag="RO454_002781"
     CDS             15843..16745
                     /gene="dppC"
                     /locus_tag="RO454_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDY0934591.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16756..17739
                     /gene="dppD"
                     /locus_tag="RO454_002782"
     CDS             16756..17739
                     /gene="dppD"
                     /locus_tag="RO454_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDY0934592.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17736..18740
                     /gene="dppF"
                     /locus_tag="RO454_002783"
     CDS             17736..18740
                     /gene="dppF"
                     /locus_tag="RO454_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDY0934593.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18770..20041)
                     /locus_tag="RO454_002784"
     CDS             complement(18770..20041)
                     /locus_tag="RO454_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDY0934594.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
BASE COUNT         4911 a         5032 c         5373 g         5154 t
ORIGIN      
        1 cttattaagc tggaatagcc acgggtgctt gaggctgttt gtctcaggta ttcaccgaaa
       61 ggcagacaga gaaaagcccc acctgactat aaatcaaagt gaggctaccc tatgcctgaa
      121 caccataagg ttagcctctt actcgttgga aatcaacaca ggaggctggg atgccgcaga
      181 aatatagatt actttcttta atagtgattt gtttcacgct tttatttttc acctggatga
      241 taagagattc actgtgtgaa ttgcatatta aacaggagaa ttatgagctg gccgcgtttt
      301 tagcctgcaa attgaaagag taagaatctt cggcgggaag ttattcccgc ctttcttacg
      361 gcgttacgca ttctcattac acccaaattt attcttcaca aaaataataa tagattttat
      421 tacgtgaccg attatttatt tcctgaaaaa caaataaaaa aatccccgcc aaatggcagg
      481 gatcttagat tctgtgcttt taagcagaga ttacaggctg gttacgttac cagctgccgg
      541 gcctttagcg ccgctttcga tggtgaagga cactttctga ccttcgtcca gagatttgta
      601 accatcgttc tggatagcag agaagtgtac gaacacgtct ttagagccat cgtcaggagt
      661 gatgaagccg aagcctttgt cagcgttgaa ccattttacg ataccagtca ttttaccgga
      721 catagtgtat tacctttaat aattaattgt gcctttcggc gatatggcgt gctttacaga
      781 ttttgaagcg ttaaaggaat gtgcactacg aggggtatca acgataactc ttgaagggac
      841 ttgccttact acactgcttt aatggtctgt acgtcaaacc gttgatgtgc attaagccac
      901 gcattggtgg gtgatgcaac aattattttt catatttatg attaatcggt tggaaaacgg
      961 tcctgttatc aggaccgtaa acagaaataa agtggataaa agcctattcc atcaactgct
     1021 tacttaatgc cgggttggct tgaatcaaac gcatcaattt tagttcggca cttgaaggct
     1081 tcacgcgtct ggattcccat tcctttacca tggcgactga tacgcccaaa acccgggcga
     1141 aatcatcgat ttttaatcct gtcccttttc gtaactgctc aatttcggta caggacgttg
     1201 ttctgtgcgt cagggtaatt ttctgcgttt catctttaaa aacaatctgt tccaggctgc
     1261 tcaacagctc atgcattgga tctttatatt ccattgaaaa ctcctcaaat cacactgcgg
     1321 gatcgtgaat tgcatcgaag ctcattaaga atagtcggga aaaacagaca cagcggggtg
     1381 tagtcagggc atactctgac tatgattcga ttcagccagt catttagcgt gacttatagc
     1441 cacctaacga tctgataaat gccgatatat ctcttaccac tcccggtttg taaagatttc
     1501 gaaaatctaa cgttctgcga aggcgtaaat ctctggctac aaaaaaggca acggcaatat
     1561 cattttttaa caatgaaaca acaaattaac cttgtttcat tttctccatg attttaccag
     1621 gaagattatg tagaaagggt atcctgcaag cctgtcctgg actatccttg tcacgtcaga
     1681 cacgcgtgtg tcgttgtgcg ctttttttgg gtgaaaggag taagaaaatg gcgacaggaa
     1741 agtcctgctc tcgctggttt gcgcctcttg cggcgttatt aatggtagtt agcctgagtg
     1801 ggtgttttga taaagaaggc gatcagcgta aagcgtttat cgacttcctg cagaatacag
     1861 tgatgcgtag cggtgaacgt ctaccaaccc tgactgccga tcagaaaaaa cagtttggtc
     1921 cttttgtctc tgattacgcg attctgtatg gttattctca gcaggtgaat caggcgatgg
     1981 attccggtct gcgcccggtt gtagacagcg ttaacgcaat tcgcgtaccg caggattacg
     2041 ttacgcaaag cggtccgctg cgtgaaatga acggttcgct gggtgtgctg gcgcaacagc
     2101 tgcaaaatgc gaagttgcag gccgatgctg cgcactctgc gttaaaacag agtgatgacc
     2161 tgaaaccggt ctttgatcag gcattcacca aagtggtaac gacgccagct gatgcattgc
     2221 aaccgttgat tccggcggca caaactttca cgcaacaact ggtgatggtt ggagactaca
     2281 ttgctcagca aggtactcag gtgagctttg tcgcaaatgg catccagttc ccgacttcac
     2341 agcaggcaag tgaatataac aaactgattg cgccattacc ggcacaacat caggcgttta
     2401 atcaggcctg gactacagca gttactgcaa cccaataaag agtaaaagcc cgagcgatta
     2461 tcgggcttta ctcctcgcag tcgcggctta gtccacgacg tgcggattca cacagttctt
     2521 ctcaaccttt ccttgtaacg cattaatcaa attatccacg gcacaggcgg ccatgccata
     2581 acgcgtctca tgggtggcag atccaatatg cggtaatgcg acgacgttgg ccattgagag
     2641 caacggcgaa tctacggaca gtggctcttg ttcgaagaca tccagcccgg cggcgtgaat
     2701 ttcccctttc tgcaatgcag caatcagtgc attttcatca accaccgggc cacgtccggc
     2761 attaatgaaa atggcggagg atttcatttt ggcgaattgt tctgcgccaa acagatgatg
     2821 cgtctcatcc gttaacggca ggatcaggca aacgaaatct gactcttgta acagagtatc
     2881 caaatcgcag tagcgggcgt tgaagcgttc ttctgcttct ttatggtggc gacgcgcgtt
     2941 atagaggatg ggcatgttga agccaaagtg cgcacgttgt gccagcgcca tgccgatccg
     3001 tcccatcccg acaatgccca gtgttttatg gtgaacgtca gtgccgtacc agtccgggcc
     3061 tatactcgcg gtccattcgc ctgcttttac ccgttctgct acctccacaa cccgacgagc
     3121 ggtagacaac accagcgcca tcagcgtatc ggcgacggtt tctgttaata cggttggtgt
     3181 gtgcatcagc agaatttttc gggcggtaag cgcatcgaca tcaaaattgt catagccgac
     3241 ggagatcgtt gatgtggcac gcagtttcgg cattttttcc agcaatgcgg catcaacatt
     3301 ctcatttgaa cccagcaagc cttcagcttc ggcaaaaatt gctgcatttt gttcgacggt
     3361 ttgtgggctg aggtttgcta cctggtgaac ggtgaaatgt tcttgcaggc gttgcagtaa
     3421 atcatcaggt aaggctttgt agaggataac ggacggcttc atgcttctct ccattcactg
     3481 ataaggcgat caccttagcc tgaaatatga tgaaggaaaa ctgaagaatg ccaggtgatt
     3541 tctgcatcac ctgacatgaa agggattaca gtgggcttaa ggtaatttct acacggcggt
     3601 tttgcgcctt accttctgcg gtgctgttgc tggcgattgg gttagccggg ccaaggccct
     3661 gagtacggat gcggctggcg tccacgccct gggtgatcaa cgcgctggca acggaatccg
     3721 cacgctgctg ggagagacgc atgttcaggt cgtgactccc cgtgctgtcg gtataaccaa
     3781 tcacgttaac cgccgttttc ggatactctt tcagtaccat tgccacgccg gtcagggtgt
     3841 tagcgcccgc cggtttcagg gttgcgctgc tgctgtcgaa ggtcacattg ttcggcatat
     3901 tgaggataat gttatccccg ctgcgggtta cgctaacacc agtgccgcgc attttgtcac
     3961 gcagcttcgc ttcctgcaca tccatgtaat aaccaacgcc gccgcccaga gctgcgcctg
     4021 ctgctgcgcc aatgagcgcg cctttaccgc gatctttctt cgaagaagag agcgcaccaa
     4081 tacccgcgcc aacgagagag cccagacctg cgccgatagc agatttacct gcttcgcgtt
     4141 cgccggtgta agggttagtt gtacagccag ataccgccag agcgccactc actacggcgg
     4201 caataagata aacacgtttc ttcattgtta atccttaata acctttttat tctttgccac
     4261 gggttccgtg gcgggagatt atgccgcgtg agcatgaaga ttattcctgg gaatactcgg
     4321 aaatttgtaa gtaatattta actgctcaat acatctaacc tttcaggagt cttcggtttg
     4381 gccaactcat cctcacgata ttccgttctg actgccgccc actgggggcc tatgctggtt
     4441 gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt tagccacagg aatggaaaac
     4501 tccttgcaga gcgcggttcg cgaccaggtt cacagcaata cgcgggtgcg atttccaatg
     4561 gtgcgcaaag gctttcttgc gtcaccggaa aatccgcaag gcattcgtgg gcaggatgaa
     4621 tttgttcgcg tgagttggga tgaggcgctg gagcttattc accatcaaca taaacgcatt
     4681 cgtgaggctt atggtccggc atcgattttt gctggttcct acggctggcg ttcaaacggc
     4741 gtgctgcata aggcctcgac attattacaa cgctatatgg cgttggcagg cggttatact
     4801 gggcatctgg gggattattc gaccggcgcg gcacaggcga tcatgccgta tgtcgtgggt
     4861 ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg tgctggaaca tagcgatgtc
     4921 gtggtgctgt ggagtgctaa cccactcaat acgctgaaaa ttgcgtggaa tgcatccgat
     4981 gagcaggggc tttcttactt ttccgcactg cgtgacagcg ggaaaaagct gatctgcatt
     5041 gatccaatgc gatcggaaac cgtcgatttc tttggcgata aaatggagtg ggtggcaccg
     5101 cacatgggca ccgatgttgc gttgatgctg gggatcgccc atacgctggt ggaaaatggt
     5161 tggcacgacg aagcgtttct ggcgcgttgc accacaggtt atgctgtctt cgcctcttat
     5221 ttgctgggcg agagtgacgg aatagcgaaa aacgccgaat gggcggcaga gatttgtggt
     5281 gttggcgcag cgaaaatccg cgagctggcg gctattttcc accaaaatac aaccatgctg
     5341 atggcaggct ggggaatgca gcgccaacag tttggtgagc aaaaacactg gatgatcgtc
     5401 acgctggcgg caatgttggg gcaaatcggc acacccggcg gcggttttgg tctttcttac
     5461 cattttgcca atggtggtaa ccccacgcgc cgcgctgcgg tgctctcttc catgcagggc
     5521 agcttgccgg gtggcaccga tgcggtagat aaaatccctg tcgcccgcat tgttgaagca
     5581 ctggaaaacc ctggtggcgc atatcaacat aacggtatgg accgacattt cccggatatt
     5641 cgttttatct ggtgggcggg tggtgccaac tttactcatc atcaggatac caatcgcctg
     5701 atccgtgcct ggcaaaaacc ggagctggtg gtgatctccg aatgcttctg gacggctgcg
     5761 gcgaaacacg cggatatcgt tctgcctgcg accacctctt ttgagcgtaa tgatctcacc
     5821 atgaccggtg attacagtaa tcagcatctg gtgccgatga agcaagtggt gccgccacgc
     5881 gatgaagcgc gtaatgattt tgatgttttt gccgagttga gtgaacgttg ggagaagggc
     5941 ggttatgcac gttttacgga aggaaaaagt gagctgcaat ggctggaaac gttttataac
     6001 gttgcccggc agcgcggggc aagccagcag gttgaattgc cgccatttgc tgagttctgg
     6061 caagccaacc agttaattga gatgccggaa aacccggaca gtgagcggtt tattcgcttc
     6121 gccgattttc gccgcgatcc gcaggcgcat ccattaaaaa ccgccagcgg taagattgaa
     6181 atcttctcgc agcgtattgc cgattacgct tatccggatt gcccggggca tccaatgtgg
     6241 ctggcgccgg acgaatggca gggtaatgcc gaaccggaac agttgcaggt actttctgct
     6301 catccggcgc atcgtctgca cagccagctg aattacagtt ctctgcgcga attgtacgcg
     6361 gtggcaaatc gtgagcctgt caccattcat cctgacgatg cccagacgcg cggcatacaa
     6421 gatggcgata ctgttcggct gtggaactca cgcgggcaaa ttcttgccgg cgcggtcatt
     6481 agcgagggaa ttaaacctgg cgtgatttgt attcacgaag gcgcatggcc ggatttggat
     6541 ttaaccgctg acggcatttg taaaaacggc gcagtgaacg tgctgaccaa agatctcccc
     6601 agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc tggcatggct ggaaaaatac
     6661 aacggtccgg aactgacagt tacagcgttt gaaccaccgg ccagctcata atccaggtgg
     6721 gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg aaaaccctgc gcatggtaga
     6781 aatctatcgc cggttgattt ttttgataaa cctccagcat cagatgggga tggcgctgct
     6841 gcacatactg catcagcgcc ttaccaatac cgcgcctgac ggccttcggt gcgacaaaca
     6901 ttgctgccag aaatcggcct tccataatgc tgacaaaacc gagaagctta ccgtcttctt
     6961 cccagaccca gttttgcgcg ttggcaagat acgcatcccg caccagcgga atgcagtcac
     7021 gccagtaact cgattttata aagggatgcc cccaggttgt actttccagc cacagttcga
     7081 ggagcgcggg gagttctgaa cgttgcgctt cccgaatcat ggtttatttc ccggatggca
     7141 acagcagcca accacatgat cattcaccag cccacatgcc tgcataaagg agtaacagat
     7201 tgtggtaccg acaaacttaa aaccacgttt ttttagtgcc ttagataggg cgtcggaggc
     7261 gggtgtagat gtggggattt cgctcaacgt tgtggcttgt gtcacctgtg gttgatgatt
     7321 gacaaacgac cagacaaagt cggcaaacgg ttcgccgttc tgttccattt gcaggtacgc
     7381 ccgcgcatta ccaataattg cctgaatttt cccacgatgg cggataatcc cggcgtcctg
     7441 taccagtctt tcgacatcct cttcctgcat tgcggcaact ttcaccggat cgaactgatg
     7501 aaagcatgcg cgatagtttt cgcgtttttt gaggacggtg atccacgata acccagcctg
     7561 ctgcccttcc aggcagatca tttcgaacag ttttttactg tcagtttcag gcacgcccca
     7621 ttcattatca tggtaggcaa tataaagcgg gtcctgactc acccagccgc aacgttccat
     7681 actttccctc gctatgtacc cgatcgtaaa aatttcagcc gcttttcttg acgcatcggt
     7741 gaaaaagtca atatttaata cagggctact atgcccgata tccttcatac aatgacaata
     7801 atattgccga attcggctct tccctggagt cgtgttaatg atcataaaaa aaagcggtgg
     7861 tcgctggcag ctaagcctgc ttgcgagcgt ggtaatcagt gccttttttc tcaacacagc
     7921 ttacgcctgg caacaagaat atatcgttga tacgcaaccc ggacattcca cagagcgtta
     7981 cacctgggat agcgatcatc aacctgatta caacgatatt ttgtcgcaac gtattcaaag
     8041 tagccaaagg gcgctgggac tggaagtcaa tctggcggaa gaaacccctg tggatgtgac
     8101 cagcagtatg agtatgggct ggaattttcc tttatatgag caggttacaa ccggcccggt
     8161 cgcggcatta cattacgatg gcacaaccac ctcgatgtat aacgagtttg gcgacagtac
     8221 taccacgctg gccgatccgt tatggcatgc cagcgtgagt accttaggct ggcgtgttga
     8281 ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc tataaccagc aatttggcga
     8341 gaatatctgg aaggcgcaat caggcctgag ccggatgacg gcgacaaacc agaacggcaa
     8401 ctggctggat gtcaccgtag gcgctgatat gttgctcaat caaaatattg ctgcctatgc
     8461 tgcgctatct caggcagaaa ataccactaa caatagcgac tatctgtata cgatgggggt
     8521 tagtgccaga ttttaacgtt atagatacaa ttgaaaccca taaataacaa ctaatgtggc
     8581 gatagtgggg actaacttac taaataataa atttggcgaa taattgtcga gtcattcatt
     8641 cctgaactaa tgcatttcat tccgttctga tggcatttca tgccgttttt ccccaggcat
     8701 aaagcgcact tcgttatggt tgtcagcaga gatttttcct ttttattact gcaggaatac
     8761 tgccatgaca ccttcaaatt atcagcgtac tcgctggctg acactcatcg gtactatcat
     8821 tacccagttt gctctggggt cggtttatac ctggagtctg tttaacggtg cgctttctgc
     8881 caagctgggg gctcctgtaa gccaggttgc tttctctttc ggcttgttaa gtctggggct
     8941 ggcaatttcg tcttctgttg cgggcaaatt gcaggaacgt tttggtgtta aacgcgtcac
     9001 tatggcttcc ggcattttgc tgggattagg tttcttcctg acagcacatt ccaacaacct
     9061 gatgatgctg tggttaagcg ccggtgtgtt ggttggtctg gcggatggtg cgggttacct
     9121 gttgacgctc tctaactgcg tgaagtggtt tccggagcgt aagggactta tctctgcttt
     9181 tgctatcggt tcttatggtc tgggcagcct gggttttaaa tttatcgaca cgcacctgct
     9241 cgaaacggtg ggtctggaaa aaacctttgt tatttggggc gcgattgtac tggtgatgat
     9301 tgtctttggc gcaacgttaa tgaaagatgc gccgaagcag gaagtgaaaa ccagcaatgg
     9361 tgtggtggag aaggactaca ccctggcaga gtcgatgcgt aaaccgcagt actggatgtt
     9421 agcggttatg ttcctgactg cgtgcatgag tggtctgtat gtgattggtg tagcgaaaga
     9481 tatcgctcaa agtctggcgc atcttgatgc aatttccgca gccaatgctg tgacggttat
     9541 ttccatcgcc aacctttctg gtcgtctggt gctgggcatt ctgtctgaca aaatcgcccg
     9601 tatccgtgtt atcaccattg gtcaggtgat tgcgctggtg ggtatggcgg ccctgctgtt
     9661 tgcaccattg aatgcagtga cgttctttgc agcgattgcc tgtgtggcat ttaactttgg
     9721 tggcacgatt acggtgttcc cgtcactggt cagtgagttc ttcggcctca ataacctggc
     9781 gaaaaactac ggcgtcattt acctcggttt cggtatcggc agcatttgtg gttcgattat
     9841 cgcttcactg tttggcggat tctatgtgac tttctacgtg attttcgccc tgctgattct
     9901 gtcactggcg ctttctacga ccattcgcca gccagagcag aaaatgttgc gtgaggcgca
     9961 tggctccctt taatgctcag ccgtgagcta ctgggcttca gcttcagtgt gcatcaaacg
    10021 catcaacgaa atgtataaac aataacgcgt aaaatttggg ccagttaact ggcccttttt
    10081 actgcttttt gtaacgctaa agtctctttt caaacttgca tttttgtaaa tttgtgcttc
    10141 gtgcacactc tttccccaca ctttttccct ttgctgtggt ctacttattc gcgcgtgtag
    10201 attttactta tctgactacc tccgcacttt tcccctgcct ggcctgaaaa gccactaagc
    10261 agggtgttat cacctgtttg tccagggttt gtttgcatga gatacatcaa atcgattaca
    10321 cagcagaagc tgagcttttt gcttgcaatc tatattggcc tttttatgaa tggcgcggtt
    10381 ttttaccgcc gctttggcag ctatgcgcac gattttaccg tctggaaagg catttctgct
    10441 gttgttgaac tggccgccac cgtactggtg accttctttt tactgcgtct gctttcgctg
    10501 tttggccgcc gcagctggcg tattctggca tcgctggtgg tgctcttttc tgcaggtgcc
    10561 agctattaca tgaccttcct taatgtagtc attggttatg gcatcatcgc atccgtcatg
    10621 accaccgata tcgacctgtc aaaagaagtt gttggtctga actttattct ctggttaatc
    10681 gccgtaagcg cgttgcctct tatccttatc tggaataacc gctgtcgtta caccttgctc
    10741 cgacaactgc gaaccccagg gcaacgtatt cgcagcctgg cggtcgtcgt actggcaggt
    10801 attatggttt gggcaccgat tcgtttgctg gatatccagc agaagaaagt ggagagggcg
    10861 acaggtgttg atttgccgag ttatggcggc gtcgtcgcga actcttatct gccatcaaac
    10921 tggctttcgg cattaggtct ttatgcctgg gcgcgggtcg atgaatcttc cgataacaat
    10981 tctctcctta accctgcgaa gaagtttacg taccaggctc cacaaaacgt tgatgataca
    11041 tatgtcgttt tcattattgg tgaaaccacg cgctgggatc atatgggcat tttcggctat
    11101 gaacgcaata ccacaccgaa actggctcag gagaaaaatc tggcggcgtt ccgtggttac
    11161 tcctgtgata ctgcaaccaa actctcactg cgttgcatgt ttgtacgtca gggtggagca
    11221 gaagataatc cgcagcgcac attaaaagaa cagaacattt tcgcggtatt gaagcagtta
    11281 ggattcagtt ctgatctcta cgctatgcaa agcgaaatgt ggttctacag caatacgatg
    11341 gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc cacgtaaccg tggcaaaccg
    11401 gtagatgata tgttgctggt agacgaaatg cagcaatcgc tggggcgcaa cccggatggt
    11461 aagcatctga tcattctgca taccaaaggc tcacacttta actacaccca gcgttacccg
    11521 cgcagctttg cgcagtggaa gccggaatgt attggtgttg atagcggctg taccaaagcg
    11581 cagatgatca actcatacga caactcggtg acctatgtgg atcactttat ttccagcgtg
    11641 attgatcagg ttcgcgataa gaaagcgatt gtgttctatg cagctgacca cggtgagtca
    11701 attaatgaac gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc ggaacagttc
    11761 cgtgtgccga tgatggtctg gatgtcggat aaatatctgg aaaatccggt caatgcgcag
    11821 gcatttgcgc agctgaaaaa agaagccgac atgaaagtgc cacgccgtca tgtagagctg
    11881 tacgacacca tcatgggttg tcttggctat acttcaccgg atggtggaat taacgaaaac
    11941 aacaactggt gccacatccc gcaggcaaga gaggcggcgg ctaactaaga ggcctgctga
    12001 ctttctcgcc gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca
    12061 gtaagatgcg ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg
    12121 aaggggtcgg aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag
    12181 caggcttttt tgcatctgta actcatgagg aagagaaccc ccgggggcgg ggaggttcga
    12241 ctcgacgtag gcctgataag acgcaccagc gtcgcatcag gcaaatgtac cggcccattg
    12301 tcggatgcgg cgcgaccgcc ttatccgatc tacacaccac acatttccct gtcaaatttg
    12361 cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc tttttttata
    12421 tttgcttaaa tctttttttg cgttacttat ggctggcgct ggcattttcc gctgtgcgtt
    12481 ttagcgttca tggtattaat cgctcagata tccaccttta cctcagattt ttttcattaa
    12541 atgcataaaa cttaatcacc aaatgttgct aaatattaag ctgattgttc tggttgtgtt
    12601 ggcgtagcac aaatttctct gctgcaaata gctgtttgaa gtttttttaa tctttgtttg
    12661 tggattctca ccgttggtgt aaatcccggt tggctgtatt gacgttttca cattctgttg
    12721 acagattgta ggtcacgagg ggcattttat ggaggatccg cactgttaca ctgatgttaa
    12781 ttagtacggc atccccacct cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca
    12841 ggcagcgaca acccactgca aagggttaaa acaacaaaca tcacaattgg agcagaataa
    12901 tgcgtatttc cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg gtggctatga
    12961 ccgtcgcagc aagtgttcag gctaaaactc tggtttattg ctcagaagga tctccggaag
    13021 ggtttaaccc gcagctgttt acctccggca ccacctatga cgcctcttcc gtaccgcttt
    13081 ataaccgtct ggttgaattt aaaatcggca ccactgaagt catcccgggc ctcgctgaaa
    13141 agtgggaagt cagtgaagac ggtaaaacct ataccttcca tctgcgtaaa ggtgtgaagt
    13201 ggcacgacaa taaagaattc aaaccgacgc gcgaactgaa tgccgatgat gtagtgttct
    13261 cgttcgatcg tcagaaaaac gcgcaaaacc cgtaccataa agtctctggc ggcagctacg
    13321 aatacttcga aggcatgggc ttgccagagc tgatcagcga agtgaaaaag gtggacgata
    13381 acaccgttca gtttgtgctg actcgcccgg aagcgccgtt cctcgctgac ctggcaatgg
    13441 acttcgcctc tattctgtca aaagaatatg ctgacgcgat gatgaaagcc ggtacaccgg
    13501 aaaaactgga cctcaaccca atcggtaccg gcccgttcca gttacagcag taccagaaag
    13561 attcccgtat tcgctataaa gcgtttgatg gctactgggg caccaaaccg aagatcgata
    13621 cgctggtctt ctctattact cctgacgctt ccgtgcgtta cgcgaaattg cagaagaacg
    13681 aatgccaggt gatgccgtac ccgaacccgg cagatatcgc ccgcatgaag caggataaat
    13741 ccatcaacct gatggaaatg ccggggctga acgtcggtta tctctcgtat aacgtgcaga
    13801 aaaaaccact ggatgacgtg aaagttcgcc aggctctgac ctacgcggtg aacaaagacg
    13861 ccatcatcaa agcggtttat cagggcgcgg gcgtatcagc gaaaaacctg atcccgccaa
    13921 ccatgtgggg ctataacgac gacgttcagg actacactta cgatcctgaa aaagcgaaag
    13981 ccttgctgaa agaagcgggt ctggaaaaag gtttctccat cgacctgtgg gcaatgccgg
    14041 tacaacgtcc gtataacccg aatgctcgcc gcatggcgga gatgattcag gcagactggg
    14101 cgaaagtggg cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg
    14161 cgaaagatgg cgagcatcag acggtaatga tgggctggac tggcgataac ggggatccgg
    14221 ataacttctt cgccaccctg ttcagctgcg ccgcctctga acaaggctcc aactactcaa
    14281 aatggtgcta caaaccgttt gaagatctga ttcaaccggc gcgtgctacc gacgaccaca
    14341 ataaacgtgt tgaactgtac aaacaagcac aggtcgtgat gcacgatcag gctccggcac
    14401 tgatcatcgc tcactccacc gtgtttgaac cggtacgcaa agaagttaaa ggctatgtgg
    14461 ttgatccatt aggcaaacat cacttcgaaa atgtctctat cgaataatta aagccataca
    14521 agactgatgg caaaggcaaa actgcctgat gcgctccgct tatcaggcct acgaaaactc
    14581 tgcaatgtat tgagtttgca cgattttgta ggtcggataa ggcgtttgcg ccgcatccgg
    14641 cataaacaaa gcgctctttg tcaacaatct gcatacccgg tggcgctgtg cctctgcgtg
    14701 cagcgccatc cggcagcaat acttcattcc ctgtcccaca agggcgggaa ttgatttgtg
    14761 agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg gttatgttgc
    14821 agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt acccttctca
    14881 catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggcg ggcgaacgtg
    14941 ggatctcccc agagcgtcac gcgcaattgc tggctgaact cggcttagat aaaccgatgt
    15001 ggcagcagta tctccattac atttggggcg taatgcacgg tgatttaggc atttccatga
    15061 aaagccgaat tccggtatgg gaagagttcg tgccgcgctt tcaggccacg ctggaacttg
    15121 gcgtctgcgc gatgattttt gcgaccgcag tcgggattcc ggttggtgtg ctggctgcgg
    15181 ttaaacgtgg ttccattttc gatcacacag cggttggcct ggcgctgacc ggctactcga
    15241 tgccaatttt ctggtggggc atgatgttga tcatgctggt ttcggtgcac tggaacctga
    15301 cgcctgtctc cggtcgcgtg agcgacatgg tgttcctcga tgattccaat ccgttaaccg
    15361 gttttatgct gatcgacacc gccatctggg gtgaagacgg taactttatc gatgccgtcg
    15421 cccatatgat cctgcccgcc attgtgctgg gtactattcc gctggcggtc attgtgcgta
    15481 tgacgcgctc ctcgatgctg gaagtgctgg gcgaagatta tatccgcacc gcgcgtgcca
    15541 aaggtcttac ccggatgcgg gtgattatcg tccatgcgct gcgtaacgcg atgctgccgg
    15601 tggtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt ctgactgaaa
    15661 ccatcttctc gtggccgggt ctggggcgct ggttgattga cgcactgcaa cgccgcgatt
    15721 atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc gtcaacctgc
    15781 tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag taagggggca
    15841 tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg accccgttac
    15901 aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg gtttacgtcg
    15961 tcatcgtgct gtttatcgcg atctttgcca actggattgc accctataac ccagcggaac
    16021 agttccgcga tgcactgctt gccccgcctg cctggcagga aggcggcagc atggcgcact
    16081 tgctgggcac tgatgacgtg gggcgtgatg tgctgtcgcg gctgatgtac ggcgcgcgct
    16141 tgtcgctgct ggttggctgt ctggtagtgg tgttatcgct gattatgggc gttattctcg
    16201 gcctgatcgc tggttacttt ggcggtctgg tcgataacat catcatgcgc gtggtcgata
    16261 tcatgctggc gctgccaagt ctactgctgg cactggtgct ggtggcgatt ttcggcccgt
    16321 cgattggtaa cgccgcgctg gcgctaacct tcgttgcgct gccgcattat gtgcgcttaa
    16381 cccgcgccgc cgtgctggtg gaagttaacc gcgattacgt caccgcgtct cgcgtggcgg
    16441 gtgccggggc aatgcgccag atgtttatta acatcttccc gaactgcctt gcgccgctga
    16501 ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct cttggtttcc
    16561 tcggcatggg cgcgcagccg ccaacgccgg agtggggcac catgctctct gacgtgttgc
    16621 agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc ctgctgacgg
    16681 tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc aaactgaagc
    16741 agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac
    16801 gaaagcgcac cgttccgcgc tgtagaccgc atcagctaca gcgtaaaaca gggtgaagtg
    16861 gtcgggattg tgggtgagtc cggctctggt aaatcggtca gttcgctggc gattatgggg
    16921 ctgattgatt atccgggccg cgtaatggcg gaaaagctgg agtttaacgg tcaggatttg
    16981 cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc aatgatcttc
    17041 caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat tatggaagcg
    17101 attaaggtgc atcaaggcgg caacaaaagt acccgccgtc agcgagcgat tgacctgctg
    17161 aatctggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca tcagctttcc
    17221 ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc aaaactgctg
    17281 attgccgatg aaccaaccac tgcgctggac gtgaccattc aggcgcaaat tatcgaactg
    17341 ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca tgacctggcg
    17401 ctggtggcgg aagcggcaca taaaattatc gtgatgtatg caggccaggt agtagaaacg
    17461 ggcgatgcgc acgctatctt ccatgcgccg cgtcacccgt atactcaggc attgctgcgt
    17521 gcgctgccgg aatttgctca ggacaaagaa cgtctggcgt cgttgccagg tgtcgttccc
    17581 ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta cgccactgac
    17641 aaatgccgtg ctgaagaacc ggcgctgaat atgctcgctg acgggcgtca gtccaaatgc
    17701 cattacccac ttgatgatgc cgggaggccg acactatgag tacgcatgag gccaccccgc
    17761 aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag aaaggcatgt
    17821 tcgcgccgga acgtctggtg aaggcgctgg atggcgtttc gtttaacctt gaacgtggca
    17881 aaacgctggc agtagtaggt gaatctggct gcggtaaatc gaccctaggt cggttgctga
    17941 cgatgattga aacgcccacc ggcggcgaac tgtattacca ggggcaggat ctgctcaagc
    18001 acgatccgca ggcgcagaag ctacgtcggc agaaaatcca gattgtcttc cagaaccctt
    18061 acggttcgct gaatccgcgt aaaaaagttg ggcagattct cgaagagccg ctgctgatca
    18121 acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg gcgaaagtcg
    18181 gcctgaaaac cgagcattat gaccgctatc cgcatatgtt ctccggcggt cagcgtcagc
    18241 gtatcgccat tgcccgaggt ctgatgctcg acccggatgt ggtgattgcc gatgagccgg
    18301 tttccgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg gatttgcagc
    18361 aggaactggg gctatcttat gtctttatct cgcacgatct gtcggtggtg gagcacattg
    18421 ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaaggggacg aaggaccaaa
    18481 tcttcaataa cccgcgccac ccgtacactc aggcgctgct ctccgcgaca ccacgcctga
    18541 acccggacga tcgtcgcgaa cgcatcaaac ttaccggtga actgccaagc ccgctgaatc
    18601 caccgccagg ctgcgccttc aacgcccgct gtcgtcggcg cttcggcccc tgcatccagt
    18661 tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgcc gttgatcagg
    18721 atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt tactcaatca
    18781 acttcaacag tggcgacaag cataacaaaa tgccgtacag cagaatcatc caggctttaa
    18841 acccgcgaag tttttccagt tgtgagactt tatagatcag gaaaaacgga atgaggcacg
    18901 acacaatgcc atataacggg cttcccaact gaaagaacac cagtaccgat acacgaaacg
    18961 aaacccaaat cgtcaacgta atgacgataa aagtgcagat cgccagagtc agcacgcgtg
    19021 agttaatttt cctggtatca ataattcggc ttaacagatt gagaataatg cctttaatgg
    19081 cctcgtggaa accgagataa atgccaaaga atgcagtcag tacggcaaag atattcagca
    19141 ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagt gccagtgccg
    19201 agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa
    19261 aggcaaaaaa caggatcacc gcgatgagcg tgacataact aattcggtgg gtacgcagcg
    19321 cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata ttcattgggt
    19381 taagtacctg aataaatacc gcggaaaaga agcaaaatgg aatagtaagc aggacatcgc
    19441 ggaaaaagtc ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca
    19501 tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggt ccggaaatct
    19561 taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa atggcgactt
    19621 tataaaatag agattgagaa agatcggcat cggttaaacc gaaggttttc aggtacgagg
    19681 cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt
    19741 agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt
    19801 agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgt actacccagg
    19861 tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt
    19921 gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc
    19981 cgaagtcgta gcgtgtaaac ggcaatttct gatcgtgttt cggtagtgtg ttgtgctgca
    20041 taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag tttctcttgc
    20101 gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg
    20161 gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactactagt ctggagtcag
    20221 cccggatatg agctgcaact attgcaaata aagaaattaa gctggaaggc atcacgcaat
    20281 gaggaagatt gccgggtgct gacggcaaaa agcaatcgta aaatcagcgg caagccggaa
    20341 gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag
    20401 agagaaaacc cccgcacgtt gcaggtatgc acctgacaac atcacggggg ccattcttgc
    20461 atctagacca
//
LOCUS       DAPQZD010000079        20206 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPQZD010000079 DAPQZD010000000
VERSION     DAPQZD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.079"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(124..200)
                     /locus_tag="RO454_002785"
     tRNA            complement(124..200)
                     /locus_tag="RO454_002785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Arg,seq:acg)
     gene            complement(204..296)
                     /locus_tag="RO454_002786"
     tRNA            complement(204..296)
                     /locus_tag="RO454_002786"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Ser,seq:gct)
     gene            complement(612..797)
                     /gene="csrA"
                     /locus_tag="RO454_002787"
     CDS             complement(612..797)
                     /gene="csrA"
                     /locus_tag="RO454_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDY0934595.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1032..3662)
                     /gene="alaS"
                     /locus_tag="RO454_002788"
     CDS             complement(1032..3662)
                     /gene="alaS"
                     /locus_tag="RO454_002788"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDY0934596.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3791..4291)
                     /gene="recX"
                     /locus_tag="RO454_002789"
     CDS             complement(3791..4291)
                     /gene="recX"
                     /locus_tag="RO454_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDY0934597.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4360..5421)
                     /gene="recA"
                     /locus_tag="RO454_002790"
     CDS             complement(4360..5421)
                     /gene="recA"
                     /locus_tag="RO454_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDY0934598.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5501..5998)
                     /gene="pncC"
                     /locus_tag="RO454_002791"
     CDS             complement(5501..5998)
                     /gene="pncC"
                     /locus_tag="RO454_002791"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDY0934599.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            6180..6419
                     /locus_tag="RO454_002792"
     CDS             6180..6419
                     /locus_tag="RO454_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934600.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            complement(6458..7543)
                     /gene="mltB"
                     /locus_tag="RO454_002793"
     CDS             complement(6458..7543)
                     /gene="mltB"
                     /locus_tag="RO454_002793"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDY0934601.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7800..8363
                     /gene="srlA"
                     /locus_tag="RO454_002794"
                     /gene_synonym="gutA"
     CDS             7800..8363
                     /gene="srlA"
                     /locus_tag="RO454_002794"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDY0934602.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            8360..9319
                     /gene="srlE"
                     /locus_tag="RO454_002795"
                     /gene_synonym="gutE"
     CDS             8360..9319
                     /gene="srlE"
                     /locus_tag="RO454_002795"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDY0934603.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9330..9701
                     /gene="srlB"
                     /locus_tag="RO454_002796"
                     /gene_synonym="gutB"
     CDS             9330..9701
                     /gene="srlB"
                     /locus_tag="RO454_002796"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDY0934604.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9705..10484
                     /gene="srlD"
                     /locus_tag="RO454_002797"
     CDS             9705..10484
                     /gene="srlD"
                     /locus_tag="RO454_002797"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDY0934605.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10590..10949
                     /gene="gutM"
                     /locus_tag="RO454_002798"
     CDS             10590..10949
                     /gene="gutM"
                     /locus_tag="RO454_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDY0934606.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11016..11789
                     /gene="srlR"
                     /locus_tag="RO454_002799"
                     /gene_synonym="gutR"
     CDS             11016..11789
                     /gene="srlR"
                     /locus_tag="RO454_002799"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDY0934607.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11782..12747
                     /gene="gutQ"
                     /locus_tag="RO454_002800"
     CDS             11782..12747
                     /gene="gutQ"
                     /locus_tag="RO454_002800"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDY0934608.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12744..14258)
                     /gene="norR"
                     /locus_tag="RO454_002801"
     CDS             complement(12744..14258)
                     /gene="norR"
                     /locus_tag="RO454_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDY0934609.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14445..15884
                     /gene="norV"
                     /locus_tag="RO454_002802"
     CDS             14445..15884
                     /gene="norV"
                     /locus_tag="RO454_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDY0934610.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15881..17014
                     /gene="norW"
                     /locus_tag="RO454_002803"
     CDS             15881..17014
                     /gene="norW"
                     /locus_tag="RO454_002803"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDY0934611.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(17236..19488)
                     /gene="hypF"
                     /locus_tag="RO454_002804"
     CDS             complement(17236..19488)
                     /gene="hypF"
                     /locus_tag="RO454_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDY0934612.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19641..20168)
                     /gene="hydN"
                     /locus_tag="RO454_002805"
     CDS             complement(19641..20168)
                     /gene="hydN"
                     /locus_tag="RO454_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDY0934613.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4813 a         5608 c         5106 g         4679 t
ORIGIN      
        1 tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca
       61 tcgggaaact tactttactg cagatttttt gataccgcca ctaaagccat atcaagtaag
      121 agatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
      181 tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct
      241 tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac
      301 gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat
      361 attactttct gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact
      421 tcacgctcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaacggag
      481 gtctgacaga tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa
      541 gcgaaaagac aatggagtga aagagaaatc ttgagggtgc gtctcaccga taaagatgag
      601 acgcggaaag attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc
      661 tcttcacggt gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc
      721 tttaccccta aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga
      781 cgagtcagaa tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat
      841 cccggcatta tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatctaa
      901 gcagtcatgg cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct
      961 gactttatcg ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc
     1021 tgacgcttat attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca
     1081 ggtaaggccg cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg
     1141 ccgcccacct gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg
     1201 tccttagata cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata
     1261 attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca
     1321 acaccgctaa gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa
     1381 agatttgcgc tctcctgtgc ggcagcttgt tctttaagct gttgtaactc tttttccagc
     1441 tgacgcgtac gttccagtac tgaacgcact ttatcagcca gattattgct atcgcctttc
     1501 agcagatgcg cgacttcgct taagcgatcg ctgtctgcat gaacggtggc gatagcgcct
     1561 tctccggtta ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg
     1621 cggaacagac caatatcacc agtgcggctg gcgtgagtac cgccacacaa ctcggtggag
     1681 aaatcaccca tgctcagcac gcgtacgcgc tcatcatact tctcgccaaa cagcgccatc
     1741 gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caagttacgg
     1801 cgaatctgcg cgttcaccag gtcttcgacc gcacgaatct cttctggttt catcgcttcg
     1861 ttgtgtgaga agtcgaagcg cagcactttg tcgttaacca gtgaaccttt ctgcgataca
     1921 tgagtaccca gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga
     1981 cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga
     2041 gaacccacag caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc
     2101 accgcgaagg agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat
     2161 tccgcataga atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca
     2221 tcaaccgatt taccatcaac aaacagcgca gtcactttgc cgttcagttc cagatggtca
     2281 tagcctttaa attcagatgc actgtcaaca cggatcattg cgttgtaatc tgcaccaaag
     2341 ccgctggctt cgcgcgcacg acgacgctgc tcttccattg ctgcgtcaaa accagcttcg
     2401 tcaactttga tgttgcgctc acggcaaacg tcagccgtca ggtcaaccgg gaagccgtag
     2461 gtgtcgtaca gacggaaagc agtttcacca tccagcgtat caccagaaag ttttgccagc
     2521 tcttcatcca gcagcgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc
     2581 ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata
     2641 acgtcgatta gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatatta
     2701 ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac
     2761 ggcattacgc catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc
     2821 agcgatttat tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg
     2881 cggaacaggt cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgttcc
     2941 agacccatac cggtatctac agacggtttc ggcagcggtt ccatcgtgcc atcggcctgg
     3001 cggttgaact gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc
     3061 ggacttcccg gagggccgcc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac
     3121 gggccgcacg gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct
     3181 ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc ctacttcttt ttcccagatt
     3241 tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca
     3301 aaccattttt cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag
     3361 tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg
     3421 acgttttcca gatcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg
     3481 cgggaataat tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg
     3541 ttggtaaaca acagagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga
     3601 tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc
     3661 ataattatcc tgaaatcaag ctaacgaaat atcgctacca gccgttgcgt gtcgttaacc
     3721 gcccggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg aagtaaaatc
     3781 ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc atcagatagc
     3841 cacgatagag cagaaaacgc tggatcttaa ctttttctga aaagacagtt ggcaaaggtt
     3901 cgccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac
     3961 gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc tgacaaatac
     4021 gcgcaggtcc ataacctttg cggctacggc tggcgataaa gcgcgcaaca aatcggctgt
     4081 catcaagata gccatgttca tggcaccagg caataacgcg ctcgtaatct tctgccgtgg
     4141 catcaatctc ttccgggcca tttttgccca taatcggtgc cgcgagttta cgtcgcagtt
     4201 cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac aggcgagcat
     4261 atgccgggcg acgggatgtt gattctgtca tggcatatcc ttacaactta aaaaagcaaa
     4321 agggccgcag atgcgaccct tgtgtatcaa acaagacgat taaaaatctt cgttagtttc
     4381 tgctacgcct tcgctgtcat ctacagagaa atccggcgtt gagttcgggt tgctcagcag
     4441 caactcacgt actttcttct cgatctcttt cgcggtttct gggttatctt tcagccaggc
     4501 agtcgcattc gctttaccct gaccgatctt ctcacctttg tagctgtacc acgcgcctgc
     4561 tttctcgata agcttctctt tcacgcccag gtcaaccagc tcgccgtaga agttgatacc
     4621 ttcgccgtag aggatctgga attcagcctg tttaaacggc gcagcgattt tgttcttcac
     4681 cactttcacg cgggtttcgc tacccaccac gttttcgccc tctttcaccg cgccgatacg
     4741 acggatgtcg agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc
     4801 cgggttaccg aacatcacac caattttcat acggatctgg ttgatgaaga tcagcagcgt
     4861 gttggactgc ttcaggttac ccgccagctt acgcatcgcc tggctcatca tacgtgccgc
     4921 aaggcccatg tgagagtcgc cgatttcacc ttcgatttcc gctttcggcg tcagtgccgc
     4981 cacggagtca acgacgataa cgtctactgc accagaacgt gccagggcgt cacagatttc
     5041 cagtgcctgc tcgccagtgt ccggctggga gcacagcagg ttgtcgatat cgacgcccag
     5101 tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc
     5161 ttcgcgctgc gctgcggcga tcacctgcaa cgtcagcgtg gttttaccgg aagattctgg
     5221 tccgtagatt tcgacgatac ggcccatcgg cagaccacct gccccaagcg cgatatccag
     5281 tgaaagcgaa ccggtagaga tggtttccac atccatggaa cggtcttcac ccaggcgcat
     5341 gatggagcct ttaccaaatt gtttctcaat ctggcccagt gctgccgcca acgctttctg
     5401 tttgttttcg tcgatagcca tttttactcc tgtcatgccg ggtaataccg gatagtcaat
     5461 atgttctgtt gaagcaatta tactgtatgc tcatacagta tcaagtattt tgtagaaatt
     5521 gttgccacaa ggtctgcaat gcatacgcag tagcctgacg acgcaccgca tcacggtcac
     5581 cgctgaagca ttcccgccgg gtaatgcctt caccacgggc agtggcaaaa gcaaaccaga
     5641 cggtgccgac aggcttctct tcactgccgc catccggccc ggcgatacca ctaatagaca
     5701 cggcgtaatc agcacgagcc gctttcagtg cgcctatcgc catttccacc acgacaggtt
     5761 cgctcaccgc gccatgctgc gccagcgtct cttcgcgtac accgatcatt tgcgcttttg
     5821 cttcgttact gtaggtgaca aatccgcgtt caaaccaggc ggaactgccg gcaatatcgg
     5881 taatcacttt cgctacccaa ccgccggtac aagactcagc agttgttacg gttgcgccac
     5941 gggctttcag cgccagccca acctgttcgc ttaactgcat cagttcactt tcagtcatca
     6001 caacctctgt cagtcaaaaa tttatgccgg acaagatagc actttcccgc aaaagatggg
     6061 gacgagcgat tcattttacg agtcgcgttc cgaaaaaaga ctgttgcggc cagtcggtag
     6121 agaaaaagcg ctaccatgcc gcgttcacat tgatatgtat tcacagggag agaatagcaa
     6181 tggcaataaa gaaaacggat aacaccccac aaaacgtgct caaacaaacc acacaaatgc
     6241 gtatgtgggt catcattggc ggcatcagcg cactgatttc cgtgatatgt aaagttttat
     6301 cttctggcta tcttgaacct tttgacttcg gtcaggctgc gttaggcgtt gtctgcctgg
     6361 gatatgcgat atcgctgtcg aaaaaaatca agcaattacg ccattcgaat aaacagtaag
     6421 ataacagagg gaagtttaac ctccctcttg caaatagtta ctgcacgcgc gccagcgcca
     6481 cggcctgtcc taactgccag accgccattg cgtaatgagt gctgtggtta taacgggtaa
     6541 tagtgtagaa gttcggcaga ccataccagt actggtaacc agtgccaaca tccagacgca
     6601 gcaagctggc ctgctgatga ttccccagcg actgctgcgg cgttagtcca gctgcggcaa
     6661 gttgtgaaat gctgtacctg gttttgaagc cattcggtaa gcctggcgcc tgaccgtttg
     6721 ccatcaccgc cacctgatcg cctttcaccc agccgtgcgc tttgaaatag ttcgctacac
     6781 taccgatggc atcaaccgga tcccacaggt tgatatgccc gtcaccgctg aaatccaccg
     6841 catattgttt gtaagacgat ggcataaact gaccgtaacc catcgcacct gcaaaggaac
     6901 ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat cagcaggaag gtttccagct
     6961 cacctgaaaa atattccgcg cggcgagggt aattaaatga cagcgttgcc agcgcatcga
     7021 gaatgcgagt tttccccatc acgcgccccc agcgggtttc aacgccgata atcccgacga
     7081 taatttccgg cggtacacca tacacctgcc acgcgcgatt caacgcatct tcatactgat
     7141 tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat aaattttttg cgataacgca
     7201 gccatgcgcc gttcggacct gatggcggtt tcaccgatgt ggttggtgcc tggttatcca
     7261 tcagccgcag taccgaatcc agacgtttcg cctgggagag aatttcctgc aactgctgac
     7321 gatcgaaacc gtgtttgttc accattttgt cgatgaactg ctgggcattc gggttattgg
     7381 cgaagtcacc gcccatctgc atcacattgt gctgcggctc aagcaggaag ccgccagacg
     7441 gcgttccggt ggtcgtttca gtctcagtag gttttggctt gctgctacag gcggcaagca
     7501 acacaaaaag gggaagcaat gttacataac gacgcttgaa catgaggggt ccatttaaca
     7561 gattcaacca ggggcaagta tggtaaagca tcccgccccg cacaaggaag cggtagtcac
     7621 tgcccgatac ggactttaca taactcaact cattctcctc actaccttct ttatacaaac
     7681 tttcaaaata aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7741 atctgattag attagattgc cgtttggtaa taaaacaata aatactgaag gagagaacaa
     7801 tgatagaaac cattactcat ggcgcagagt ggttcatcgg gctgttccag aaaggcggag
     7861 aagtgtttac cgggatggta accggcattc ttccgctgtt aattagcctg ctggttatca
     7921 tgaacgcatt gattaatttt atcggtcagc atcgtattga acgatttgct caacgttgcg
     7981 ccggtaaccc tgtttcccgt tatctgctgc taccgtgcat tggtacgttt gtcttttgta
     8041 acccgatgac cctaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctattacg
     8101 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     8161 aactgtttgt ttatctcggc attgccagcg gtctgacgac gctgaatctg ccacttggcc
     8221 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttcttccgc ggctgggtaa
     8281 ccgatctgac caccgccatt ttcgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8341 gtctggcagg agcaacatca tgacacgcat tcggatcgaa aaaggaacgg gtggctgggg
     8401 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8461 ccggcctgcg attgttgaca aactggcaca gcttactggc tggcaggcta ttgacggatt
     8521 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8581 attacgctgc ggcatctatc cgaaacggcg tattcccacc attaatatcc actcgacggg
     8641 caagtctggc ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8701 agaaaacatc actgtagtgg gtgaggcgac accacaaccc tcttccgtgg gccgtgacta
     8761 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8821 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8881 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctt atcggcatca ttatggcttc
     8941 cggtcttggc gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     9001 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     9061 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     9121 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     9181 cccggtcggt ttgtcgctgg cggaagctcg tcaggacacg gttcgcgtcg gtgtcccttc
     9241 tgttctggtg agccgctttt taaccggcgc gccaactgta ctgatcgcct ggtttgtctc
     9301 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9361 gtattggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9421 gcgcgccagc ggatctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9481 cactccatcc cggtttgcaa tttacgctcg ggcggcatcg ctatccggtg accgccgttg
     9541 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9601 taagcgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9661 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9721 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9781 ggtatcgcgt ggcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9841 ttaacgccga atatggtgaa ggaacagcgt acggttttgg tgctgacgcc accagcgagc
     9901 aaagcgttct ggcgctttct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9961 tctacagcgc cggaatagca aaagcagcct ttatcagcga tttccagctt ggagattttg
    10021 accgttcgct acaggtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
    10081 tgatgattcg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
    10141 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
    10201 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcgctg atgctcggta
    10261 acctgctgaa atcgcctatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10321 tcaaaccgga gcaagtcgag cagtattaca tcgataaagt accgctcaaa cgcggctgcg
    10381 attatcagga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10441 ctggtcagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatct
    10501 tttaaggatc tccgcgcgac taatagaatg cctgatgcgc tacgcttatc aggcctacag
    10561 gacttccgcc actacatcac ggagaagtta tggtatccgc actcatcacc gtcgccgtta
    10621 tcgcctggtg tgcgcaactg gccttaggcg gttggcaaat ttctcgtttt aaccgtgcct
    10681 tcgacacact atgccaacaa gggcgcgttg gcgtgggacg ttccagcggg cgctttaaac
    10741 cacgggtcgt ggtcgccatc gcgctggacg atcagcagcg catcgtcgac accttgttta
    10801 tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg
    10861 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    10921 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttactag cttgcgaagt
    10981 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    11041 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    11101 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
    11161 tcattcgtac ttatggcggc gtggtgttga acaaagagga atccgatccg cctatcgatc
    11221 ataaaacgct tatcaacacc cacaagaaag agctgattgc agaagctgcc gttggtttta
    11281 ttcatgatgg cgactcaata attcttgatg ctggcagtac cgtgttgcag atggtgcctc
    11341 tgctctcacg ttttaataac atcacggtga tgaccaacag tctgcacatc gtcaatgcgc
    11401 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt cgtaaaaaat
    11461 cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat
    11521 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc aacgaggttt
    11581 ataccgtcag taaggcgatg tgcaatgccg cgcgcgaagt gattttgatg gctgactcat
    11641 cgaagtttgg tcgtaaaagc cccaacgtgg tttgtagtct tgaaagcgtc gataagctga
    11701 ttaccgacgc aggtatcgat ccagcgtttc gtcaggcgct ggaagaaaaa gggatcgacg
    11761 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
    11821 atgctggagt tgcaggaagc aagtcattta ccggaacgtc tgggcgatga tttcgttcgc
    11881 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    11941 ggccacattg gtaagaaaat cgccgcaacg cttgccagca ccggcacacc ggcttttttt
    12001 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
    12061 ctgtttatct cttattccgg tggcgcgaag gaactggatc tcattattcc gcgtctggaa
    12121 gacaaatcta tcgcgctgct ggcaatgacc ggaaaaccga cgtcaccgct gggcctggcg
    12181 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    12241 acctccagca ccgtgaatac cctgatgatg ggcgacgcgc tggcgatggc agtcatgcag
    12301 gcgcgtggtt ttaatgaaga agattttgca cgctcccacc cagccggggc actgggcgct
    12361 cgcttgctga acaaagtgca tcatcttatg cgtcgtgacg atgccatccc gcaggtagcg
    12421 ttaaccgcca gcgtgatgga cgcgatgctg gaacttagcc gcaccggttt aggtctggtg
    12481 gcagtgtgcg atgatcaacg actggtgaaa ggcgtcttta ccgacggtga tttacgtcgc
    12541 tggctggttg gcggcggcgc actcaccacg ccagtcaatg aagcgatgac ggtcgacggc
    12601 accacgttgc aatcgcaaag ccgtgccatc gacgccaaag aaatcctgat gaaacgaaaa
    12661 atcaccgccg caccggtagt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
    12721 gatttctatc aggccgggat tatttaatcc ttcagcccca gacgtttcgc cagccgatgc
    12781 aggttggcga cgtcggtttc cagcatccgc gcacaggcgg cccagttgtg atgattttgc
    12841 gccagtgcct gacgaatggt ttcacgctgg aacgcttctg tcgcttcacg cagattttgc
    12901 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    12961 tgttgcgcct caagaatcac ttcatcgccg ctgcgggtgg ctctcgacaa tactaccgcc
    13021 cgatgaatag catgttccag ttcgcgcacg tttcccggaa aattgtagtg ttgcagtaaa
    13081 tttcgcgctc cggcacttaa taccacgcgg gagagcccca gccgcaaacg acactgctcg
    13141 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcactgaa
    13201 agtggaaaca cgctcaggcg gtggaacaag tcagcgcgaa atcgccctgc cagtacctct
    13261 tcgcgtaagt cacggttagt cgccgccagc acgcgcacat cgacccgcaa actgcggtca
    13321 tcgccaacgc gttgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13381 aacgacaact cgccgatctc atcgagaaac agcgtgccgt tatccgccat ttcaaacttc
    13441 ccgctgcggt tactgatagc accagtaaac gcccctttca catgcccgaa caactcactt
    13501 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    13561 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgcta
    13621 attaaaacgt tgagatcgga tgccgccaca atctcaatct cttttttcaa ttgcgtcatg
    13681 cctggggaca aaccaatcat ctgcgtctgt ttcaccgctt caaacggcgc ggcatcgcct
    13741 ggcagcatat tctggctttc cagttgttca atcagtaacg cattgcttaa cgctcccgcc
    13801 gccagcgcag caatcaggcg taactcttcg tcgctgaaaa catcgaactg atcgggctgc
    13861 atcccgtcga gcgtcaaggc accgatcagg ttttgcccgg caaacaacgg caaaccaacg
    13921 caggcgtgaa ccttcagact ctcctgccct ggaatcagac cgtcataagg atcgggcaat
    13981 tcgctgtctg cgggaaagcg caccacatca cccgcgcggg caatcgcttc cagccgtgga
    14041 tgcccttcca gcgcaaagcg tctaccgagt acatccttcg ccagcccgtc gatggcaagc
    14101 ggaataaact gccgcgagtc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    14161 cgcagcgtag tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    14221 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    14281 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
    14341 cataatttta tttatagagt aaaaacaatt agataaaaaa ctggcacgca atctgcaatc
    14401 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    14461 aaaataacat tcattgggtt ggtcaacgtg actgggaagt gcgtgatttt cacggtacag
    14521 aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg
    14581 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    14641 aaatcgatct ggcggatatc gattacatcg tgattaatca tgcagaagaa gaccacgccg
    14701 gagcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt acagccaacg
    14761 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtagtgaaaa
    14821 ctggcgacac actggatatc ggcaacggca aacaactcat ttttgtcgaa acgccaatgc
    14881 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
    14941 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagactg
    15001 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
    15061 taacgccgaa aattaccgag atcctgggct ttaacttgcc agtcgatatg atagcgacct
    15121 ctcacggcgt ggtatggcgc gataacccga cgcaaattgt cgagctgtac ctgaaatggg
    15181 cggcggatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
    15241 gcatgatggc tgacgctatc gcccagggga ttgcggaaac cgacccacgc gtggcggtga
    15301 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    15361 aaggcgtact ggtcggcact tctacgatga ataatgtgat gatgccgaaa atcgccgggc
    15421 tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagcgct ttcggctctc
    15481 acggctggag tggcggtgcg gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg
    15541 aaatgtcgct tagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgtc
    15601 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgcca cagagcacgg
    15661 tgaatacggt ggttaaagaa gaaacctctg ccgccacgac ggctgacctc ggcccacgga
    15721 tgcaatgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    15781 ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctgtgcccg gaatgctctc
    15841 tcggcaaaga tgtctttgac gaactggcat cggaggcaaa atgagcaacg gcattgtgat
    15901 cattggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgcctg
    15961 tattccatta accctgattg ccgccgacag catggatgag tacaacaaac ctgacctcag
    16021 ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg caggtgaatt
    16081 tgccgagcag tttaatctgc gcctgtttcc gcacacctgg gtaacggata tcgatgccga
    16141 agcccatgtg gtgaaaagtc agaataatca gtggcaatac gacaagttag tgctggcaac
    16201 cggtgccagc gcctttgtcc cgccagtgcc cgggcgtgag ttaatgctga cgttaaatag
    16261 tcagcaagag tatcgcgcct gtgaaacaca actgcgggat gcccgacgcg tgttgattgt
    16321 tggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgggcag gcaaagcggt
    16381 cacgctaatc gacaacgctg ccagtattct ggcgtcgtta atgccaccgg aagtcagcag
    16441 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttaaaat ctcagttgca
    16501 aggactggaa aaaacagatt ctggcattct ggcaacgctg gaccgccagc gctgcatcga
    16561 agtggatgcg gtaattgccg ccaccggact gcgcccggaa accgccctgg cacgacgcgc
    16621 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga
    16681 tatttatgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctccagcc
    16741 gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
    16801 actcccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    16861 accccagcgt caggatttac gctggcaaat taataccgaa cgccagggaa tggtggcgcg
    16921 cggtgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    16981 ggcatttgga ttgttgaaaa cgttgtcgat gtaggcgagc tacagtgccc caaatgtcgg
    17041 atgcggcgct gacgcgtctt atccgaccta cagggaacgc atgtgttgat cacataatac
    17101 gtttatgtcg cattgcactg tcccaaaatg tcggatgcga cgctagcgcg tcttatccga
    17161 cctgcgggga tgaatatgta ggccggataa ggcgttcacg ccgcatccgg caatggtgtc
    17221 caaatgcaac acgctttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat
    17281 aaccccctgc cccagagaca acccgccatc gcccgcaggt aaactctgtg gaaagagcaa
    17341 tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc
    17401 gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc
    17461 ccccccctgc gccagcgcat catgaaacgc ccacgcacgt tgattaaccg gagcctgcca
    17521 gttcagccac tgctgccaga aggtggcgag atccagttga ttatccacca gcggcatcgt
    17581 caccggatgc gtcacgccgt ggcacgaagt cgccagcgcc tccagagcac aagccgcttc
    17641 accttcataa cttaacgtgg ctggcgcaca gcccagcgcc gccgctacgg catcgaacaa
    17701 ccgcccacac gatgacgcca gtggtgcatt gattccacgc tcaatggccc gcgccagcac
    17761 gctccagttt tgctgttgca cacttaccgt ttcgggataa ctctgccact ccggcacaaa
    17821 acgcaagcac tgcgtcagca ggtttcgcca cggctgcttt gcggcaagat cgccaccagc
    17881 aagcgccact gcaggcaagc cgcccaggtg ctcgcattca cgatagttca cccgcaggca
    17941 ctctcccccc cacaaagcgc cgttctcccc cataccaata ccgtcgagtg tcaacgcaat
    18001 gacatctccg ccatccagcg gccagtgatg ttctgccaga catgccgctg cgtgagcatg
    18061 atgatgcagc accgtttgcg tcggcagatt catttcgcgc gcccactggc tggagacata
    18121 gcccggatgc gcgtcatgca caacgtattg cggagtgaaa tcgtagatgt tttgcatcag
    18181 gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg
    18241 actcaacacc gcttgttcgc cgcgcaccag gcagaaggta tttttcagat ccgcgccgag
    18301 acacagcaca ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat ccggcacata
    18361 cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg
    18421 cacgatgtcg cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc
    18481 ctgttcgttg ctgatagccg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg
    18541 gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac
    18601 ttcgttaagg ccaggggcga tatcatcaca aagctcaggt acatattttt tatccaccag
    18661 cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc
    18721 cactggcaac atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc
    18781 caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc
    18841 gacaatgttg cccattttta actgtgcgat agctgcctgt aatgccgcct cttgttctgc
    18901 atgttcacca tgacttaccc attcaagatg ggggccacac tccgggcagg ccaccggctg
    18961 ggcgtggaag cgacgatcga gcgggtcaca gtactctttg tcacaagccg gacacagcgg
    19021 aaacgccgcc atcacggtaa acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg
    19081 cgggccacag tgggtacagt tgataaacgg ataacgataa cgccgctcgc ccggggtatt
    19141 catttcggca aggcaagcag ggcaagtcgc ggcatcggga actatttgcg tattcatagc
    19201 gccgcctgtg ctctggcgga tagtgaactc ggtgggcagt tgtgaccaga taaacggctc
    19261 acgctcgacg ctatcaatac gcgccagcgg cggacaatgc tgatgcaatt gaacaagaaa
    19321 ggtttccggc tcttccagca gccggacttc tacgccatcg ccgtcattac agacatcgcc
    19381 gtgaagattt aattgctgtg ccagttgcca gacaaacgga cgaaaaccga cgccctgcac
    19441 tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc
    19501 gccatcatga attgcgtaac ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac
    19561 aggtcggcga tactgcccgc ttcgtattct acgaatattt ccgggaattc ctttgatacc
    19621 agaacagttc tgtaagattt ttagaacatc agcgccgtgc ggcggcgttt ttctgcgctc
    19681 agttgttcaa gtttattacg atcgacacaa atcagcgcat gggtcgggca agccgccata
    19741 cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc ggctttgtca
    19801 gcccgtacat tcagccctgc gccgctgttg cgaatcaccg gacgcaccac cacttccatc
    19861 gcaccatatg ggcaagccac aacgcaggtt ttgcaaccaa tacaacgttc ctgcatgaca
    19921 tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca
    19981 tcttcacact gacggcagac tgtcgccgtg gaaatgttca cacctttaat gacatggatt
    20041 cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc
    20101 acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa
    20161 cggttcatca aattctccag caataacagt taatgcgccg atacat
//
LOCUS       DAPQZD010000080        20049 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPQZD010000080 DAPQZD010000000
VERSION     DAPQZD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.080"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..506
                     /locus_tag="RO454_002806"
     CDS             <1..506
                     /locus_tag="RO454_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDY0934614.1"
                     /translation="VDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNT
                     EMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGH
                     RTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDAT
                     MFDVNKK"
     gene            complement(541..1752)
                     /gene="ydcO"
                     /locus_tag="RO454_002807"
     CDS             complement(541..1752)
                     /gene="ydcO"
                     /locus_tag="RO454_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDY0934615.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            1805..2341
                     /gene="sutR"
                     /locus_tag="RO454_002808"
     CDS             1805..2341
                     /gene="sutR"
                     /locus_tag="RO454_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDY0934616.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2414..4375
                     /gene="rlhA"
                     /locus_tag="RO454_002809"
     CDS             2414..4375
                     /gene="rlhA"
                     /locus_tag="RO454_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDY0934617.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(4467..4697)
                     /locus_tag="RO454_002810"
     CDS             complement(4467..4697)
                     /locus_tag="RO454_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HDY0934618.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4876..5112
                     /gene="hicB"
                     /locus_tag="RO454_002811"
                     /pseudo
     CDS             4876..5112
                     /gene="hicB"
                     /locus_tag="RO454_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            5191..6597
                     /locus_tag="RO454_002812"
     CDS             5191..6597
                     /locus_tag="RO454_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDY0934619.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6842..7987
                     /locus_tag="RO454_002813"
     CDS             6842..7987
                     /locus_tag="RO454_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0934620.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8005..9018
                     /locus_tag="RO454_002814"
     CDS             8005..9018
                     /locus_tag="RO454_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0934621.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            9019..9960
                     /locus_tag="RO454_002815"
     CDS             9019..9960
                     /locus_tag="RO454_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0934622.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9950..10744
                     /locus_tag="RO454_002816"
     CDS             9950..10744
                     /locus_tag="RO454_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0934623.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10766..12190
                     /gene="patD"
                     /locus_tag="RO454_002817"
     CDS             10766..12190
                     /gene="patD"
                     /locus_tag="RO454_002817"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDY0934624.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12287..12382)
                     /gene="yncL"
                     /locus_tag="RO454_002818"
     CDS             complement(12287..12382)
                     /gene="yncL"
                     /locus_tag="RO454_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDY0934625.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12578..12751
                     /gene="ortT"
                     /locus_tag="RO454_002819"
     CDS             12578..12751
                     /gene="ortT"
                     /locus_tag="RO454_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDY0934626.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12837..13070
                     /locus_tag="RO454_002820"
     CDS             12837..13070
                     /locus_tag="RO454_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDY0934627.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTL
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13071..13520)
                     /locus_tag="RO454_002821"
     CDS             complement(13071..13520)
                     /locus_tag="RO454_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDY0934628.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(13517..14035)
                     /gene="mddA"
                     /locus_tag="RO454_002822"
     CDS             complement(13517..14035)
                     /gene="mddA"
                     /locus_tag="RO454_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDY0934629.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14284..14439
                     /locus_tag="RO454_002823"
                     /pseudo
     CDS             14284..14439
                     /locus_tag="RO454_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(14516..16618)
                     /locus_tag="RO454_002824"
     CDS             complement(14516..16618)
                     /locus_tag="RO454_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDY0934630.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            16860..17921
                     /locus_tag="RO454_002825"
     CDS             16860..17921
                     /locus_tag="RO454_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDY0934631.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(18034..19533)
                     /gene="ansP"
                     /locus_tag="RO454_002826"
     CDS             complement(18034..19533)
                     /gene="ansP"
                     /locus_tag="RO454_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDY0934632.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
BASE COUNT         5086 a         5010 c         5148 g         4805 t
ORIGIN      
        1 gggtagaccc gagttttgat caaagcaaat atgacagcat cgtctggaac ccaatcactt
       61 attatccggt accgaaacct tctacccagg tagggcagaa agttctggat aaaattttga
      121 actataccaa caccgaaatg aaagaagcga tagcgcagcg taaaccactg gttaccaccg
      181 ctgggccgcg tagtctgatt ttccgtgggg ccattaccgg tgtagatacc agcaaagaag
      241 ggctgcaatt ctatgaagtg gttcctgttg cattagtggt tgcggggacg caaatggcta
      301 caggccaccg taccatggat actcgcttgt attttgaagg tgagctgatt gatgcagcga
      361 ctaataaacc ggttatcaaa gtcgttcgtc agggcgaagg taaagacctg aataacgaaa
      421 gtacgccaat ggctttcgaa aatattaaac aagttattga tgacatggcg accgatgcca
      481 ccatgtttga cgttaacaaa aagtagttcc agacgcgcca tcgttagatg gcgcttttta
      541 ttatcggttt ctgttagcga ttaaattcaa caccacgtaa caaacgcctc cggcaattaa
      601 tccccaaaac gcagaaccaa tcccgaacag cgtcaatcca cttgccgtta ccagaaatgc
      661 caccaccgcc gcgtcgcgct cacgctcatt atgcagcgcc tgatacaaac tgccgccaat
      721 ggtactcaac agcgcaagac ctgccagcat ctggatccaa cttacgggca gggcagccat
      781 catcccggta atggcactac caaacagacc tgcgatcaaa taaaaaatgc cggcaacggc
      841 agcggccagc caacgttgat ctttatccgg atgcgcttcc gggctttggc aaatagccgc
      901 ggtgattgcc gcaataccga cggaataaac gccgaaaggg gaaaaaaaca gtgccagcaa
      961 tccagtaaac acaatcaacg gcgaaacagg agccgaatac ccggctgctt tcattgctgc
     1021 gatacccggt gcgttttgcg atgccatcgt caccagaaaa agggggagtg caacgctcag
     1081 actgtgagca aacgaaaaat caggggtaat ataagtgggg agaacgggtt taaagacaat
     1141 atcagttgta acaacgtcac cttgcgcggt aacgatgacg atcccaataa tcatcgcggc
     1201 aattaccgca tagcgcggcg caacggcctt ggctgccagc catgccagca acatactgcc
     1261 acacaacgta aattgaccat ccagactggc aaacgcctgt aaaccaaaac gtaataaaat
     1321 cccggcaagc attgccgccg caagcgagtg cggaataatg cgcatcagac gagcaaagag
     1381 tcccgttatg ccgcagagga ctattaacgc gttggtgaca ataaaaacgc cgatggcttc
     1441 gttaagtgtt agttcctgca atccggtgac caacaaagcc gcgccaggcg ttgaccatgc
     1501 ggtgagaaca ggtacgcgat accataatgt cagagtcagc gtactgacgc ccattgccag
     1561 ccccagcgcc gtcatccagc cagagatttg tgcagtggtg gctccggcga caatcgctgc
     1621 ttgccagatt attgccgctg aactggcgta gccaattaat accgccagaa accccgccag
     1681 tagcgtgggt ggaggaatag aaaacagacg catagtcacc tcgtgcgtta taacgtacag
     1741 gtaccgtagc atttgtccgt tataacgcac aagtgataaa cttccgtttt gccggaggag
     1801 tcgcatggaa aatctcgctc gttttttatc caccacactt aaacaactac gtcagcagcg
     1861 cggctggagt ctttcgcgac tggcagaagc gacgggcgtt tctaaagcga tgttagggca
     1921 gattgagcgt aatgaatcca gcccgacggt cgcgacatta tggaaaattg ccaccgggtt
     1981 gaacgtgcca ttttcaacat ttatttctcc gccgcagtcc gctacgcctt cagtttatga
     2041 tccacaacgg caggcgatgg tgatcacttc gctgtttcct tatgatccac aactctgctt
     2101 tgaacacttc tcgattcaga tggcacccgg tgcaataagc gaatcgatgc cccatgaaaa
     2161 gggggtaatc gaacatgtcg tcgtcattga tggacaactt gatctgtgtg ttgatggtga
     2221 atggcagagc cttaattgtg gggaaggcgt tcgatttgct gcagacgtca cgcatgtcta
     2281 tcgtaatggt ggggagcaaa ccgtacattt tcattccctc atccattacc cgcgcagtta
     2341 agcgggaaaa ctatttcgca acgtcgcgct tgtggctaaa atagccgcca tttttcagct
     2401 actggataag aaagtgaccg tatcttctca tcgtcttgaa ctgttaagcc cggcacgcga
     2461 tgccgccatt gcccgcgaag ctattttgca cggtgccgat gctgtttata tcggcggccc
     2521 tggttttggt gcccgtcata atgccagtaa tagcctgaaa gatattgccg agttggtgcc
     2581 gtttgcccat cgttatggtg caaaaatttt cgtcacgctt aacaccattt tgcatgatga
     2641 tgagctggaa cccgcgcaac ggctgattac tgacctctac cagaccggtg tcgatgcgct
     2701 gattgttcag gatatgggga ttctggaact tgatattccg ccgattgaac tgcacgccag
     2761 tacgcagtgc gatattcgta cagtagaaaa agcgaagttc ctctctgatg ttggcttcac
     2821 gcagattgtg ctggcgcgag aactgaatct tgagcagatc cgcgcgattc accaggccac
     2881 gaacgcgacc attgaattct ttattcatgg cgcattgtgc gtggcctatt cgggtcagtg
     2941 ctacatttct catgcgcaaa cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg
     3001 ccgtttgcca tacacattga aagacgatca ggggcgggtg gtttcctatg aaaaacatct
     3061 gctgtcgatg aaagataacg atcagactgc caacctcggc gcgctgattg atgctggcgt
     3121 acgctccttc aagattgaag ggcgttacaa agatatgagc tacgtgaaga atatcaccgc
     3181 ccattatcgc cagatgctgg atgccattat tgaagaacgt ggcgatctgg cgcgcgcttc
     3241 atcaggtcgt actgaacatt tctttgttcc atcgacggaa aagactttcc accgtggtag
     3301 cacagattat tttgtgaatg cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt
     3361 tatcggcctg ccggtgggcg aagtattgaa agtagcgaaa gatcatattg atgttgccgt
     3421 taccgagcca ctggcaaatg gcgatggcct gaacgtgatg attaaacgtg aagtcgtcgg
     3481 ttttcgtgcc aatacggtag agaaaaccgg agaaaatcag taccgcgtct ggccaaatga
     3541 aatgccagca gatttgcaca aaattcgccc gcatcaccca ctaaaccgta atcttgatca
     3601 taactggcac caggcactga caaaaacctc cagcgaacgt cgggtggcgg tagacattga
     3661 actgggcggc tggcaggaac aactgattct gaccctgacc agtgaagagg gcgtcagcgt
     3721 cacgcatacg ctggacgggc agttcgacga agccaataac gccgaaaaag caatgaacaa
     3781 tctgaaggat ggtctgacaa aactggggca aaccatctat tacgcccgca atgtgcaaat
     3841 taatttgccg ggggcgctgt ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc
     3901 cgccgatatg cttgatgctg cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt
     3961 tgctgatcct gcgccggttt atccgcaaac gcatctgagt ttcctcgcga acgtatacaa
     4021 ccagaaagcg cgtgaatttt atcatcgcta tggtgtgcaa ctgattgacg cggcgtatga
     4081 ggcacatgaa gagaagggcg aagtcccggt gatgatcacc aagcattgtc tgcgctttgc
     4141 cttcaatctg tgcccgaaac aggcgaaagg caatatcaaa agctggaagg cgacgccaat
     4201 gcaactggtt aacggcgatg aagtattaac gctaaagttt gattgccgcc catgcgagat
     4261 gcacgtcatt ggcaaaatca aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt
     4321 ggcatccgta agtccggatg atctgctgaa aacattgccg aagcgaaaag ggtaaaacgc
     4381 cagttttcgg gttactcacc actcatcaaa tctgcatgat attgcctgcc gggtaaggcg
     4441 tcatgccgca tccggcaaca aatgactcaa cgctgtcccg cttctggttt gcgcgatttt
     4501 tgccagtaaa aatgctcgcg caaaccttcc gccgactctt ccgccacagc acgcaattca
     4561 tcgctgtccg cttcatgacg cagctgatga tccacattct ttacccacat aaattcatgt
     4621 cctttgtgcc ctgccatgag ttgtcctgag aacagtgcac acgttaataa gacaaccgat
     4681 aacgccttcg aaaacatccc gccacctttt tctaaccttt tgccgctatg atgccgatcg
     4741 tttcttgagg ttattattca gttttgcaaa ttagagcaaa gaaattctgg aatcttcctt
     4801 cctgattttg cattgaattc tgccgttgcg gcgatttagt gctattttcg agcacattac
     4861 acacggaggt aaacttcgcc attaaatagt cacgatcact ttattgaagt gcctttgagc
     4921 gtcgcctcta atgtattgct gttaaatgct tttttacagt cagaaatcac tcagcaagag
     4981 ttagccaggc gaattggcaa acataaacag gagattactc gcctatttaa tttgcaccat
     5041 gcgacaaaaa tcgacgccgt ccagctcgcg gctaaggcgc ttggcaaaga gttatcgctg
     5101 gtgatggttt aattacagtt aacgaaaagt tgtcattttt aacaactgat atagactgcc
     5161 gaatcatctg cacataatta cgattcgata atgaaaaaat accagcagct tgcagaacaa
     5221 ttacgcgagc agattgcgtc gggtatctgg caacccggcg atcgtttgcc ttcgttgcgt
     5281 gaccaggtgg cgctttctgg catgagcttt atgactgtca gccatgccta tcagttgctc
     5341 gaaagtcagg gatatattat cgcgcgaccg caatcgggtt attacgttgc gccacaggca
     5401 ataaaaatgc cgaaagcgac agtcattcca gtcacacgag atgaagcagt cgatatcaac
     5461 acctatattt ttgatatgtt gcaggccagt cgcgatccgt cggtagttcc gtttgcctcg
     5521 gcctttcccg acccgcgact tttccccctc caacaactaa accgctcgct ggcgcaggta
     5581 agcaaaaccg ccacggcgat gagcgtgatt gaaaacttac cgccaggaaa cgcagaactg
     5641 cgtcaggcta ttgctcgtcg ctatgcctta cagggcatca ccatttctcc tgatgaaatt
     5701 gtcattaccg ccggggcgtt agaggcatta aacctcagtt tgcaagcggt aactgaaccg
     5761 ggcgattggg tgatagtaga gaatccttgt ttctacggcg cgttgcaggc gctggagcgg
     5821 ttacggctga aggcgttatc ggtagcgacg gatattaaag aagggatcga tcttcaggcg
     5881 ctggaactgg cgttgcagga gtatccggtg aaagcgtgct ggctgatgac taatagccag
     5941 aacccacttg gatttacctt aacgccgcag aaaaaagtgc ggctggtagc gttgctcaat
     6001 cagtacaacg taacgctgat tgaagatgac gtttatagcg aactttattt tggacgggaa
     6061 aaaccgctgc ctgcgaaagc gtgggatcgc cacgatggtg ttttgcattg ctcttcgttt
     6121 tcgaaatgtc tggtgcctgg ttttcgtatt ggttgggtcg ccgccggaaa acatgcacgt
     6181 aaaattcaac gcttgcagtt gatgagtacg ctttccacca gctcaccgat gcagcttgcg
     6241 ctggtggatt acctttctac tcgccgatac gatgctcatc ttcgtcgcct gcgtcgccag
     6301 cttgcggaac gtaaacaacg cgcctggcag gctttgctgc gttatctgcc tgcggaagtc
     6361 aaaattcatc acaatgacag cggttatttt ctctggctgg agctccccga gccgttagat
     6421 gcaggagaat taagcctggt ggcactgacg catcatatca gtattgcgcc gggtaatatg
     6481 ttttctaccg gtgaaaactg gtcacgtttt tttcgtttta ataccgcgtg gcagtgggga
     6541 gaacgtgaag aacaggcggt aaaacaatta ggcaaactta ttcaagaacg gctgtaatag
     6601 cgtttaattt aattcctctc aggttgggta atatgaattt cgaatagcag tcatatatcc
     6661 taactccttg tctatactcc agaagataac cttacagacg gcataatgcg cggtagctca
     6721 caacctgatt aaattttctc agggccgaag gtgtgcctgc aagccgccgt ctatggttaa
     6781 acaaggagat atttttacgg cacggcggct gaacaattaa ttacgacagg agtaagacct
     6841 tatgagcaag acatttgccc gcagcagcct gtgtgcgctc agcatgacaa taatgaccgc
     6901 tcacgccgcc gaaccgccta ccaatttaga gaatccggaa gggcgactgg atattatcgc
     6961 ctggccggga tacatcgaac gcggacaaac tgataaacag tacgactggg taacgcaatt
     7021 cgaaaaagag acaggctgcg cggtgaatgt taaaaccgcc gcgacttccg atgaaatggt
     7081 cagtctgatg gccaaagggg gttacgatct ggttacggca tccggcgatg cctcgctgcg
     7141 tttgattatg ggcaaacgcg tgcagccaat taataccgcc ttgattccca actggaaaac
     7201 gctcgatccg cgcgtggtta aaggcgactg gtttaacgtt agtggcaaag tttatggcac
     7261 accttaccaa tggggtccga acctgttgat gtacaacact aaaaccttcc cgacgccgcc
     7321 ggatagctgg caagtggttt ttgttgagca gaatctgccg gacggcaaga gcaataaagg
     7381 ccgcgttcag gcttatgatg gccctatcta cattgcggac gctgcgttgt tcgttaaagc
     7441 cactcagccg cagttgggca tcagtgatcc gtatcaactc accgaagaac agtaccaggc
     7501 ggtgctgaaa gtgctgcgcg atcaacatag tttgatccat cgctactggc atgacactac
     7561 cgtgcaaatg agcgatttca aaaacgaggg tgtggttgct tccagtgcat ggccctatca
     7621 ggccaacgcc ctgaaagccg aaggccagcc tgtcgctacc gttttcccga aggagggcgt
     7681 taccggttgg gctgacacca ccatgctaca tagcgaagcg aaacatccgg tttgcgccta
     7741 caaatggatg aactggtcat taaccccaaa agtgcagggc gatgtggcgg cctggtttgg
     7801 ctcgttacca gtagtgccgc aagggtgtaa agccagtccg ttattaggcg agaaaggttg
     7861 tgaaacaaac ggttttaact atttcgataa aatcgccttc tggaaaacgc ctgtagcaga
     7921 agggggcaag tttgttccct acagtcgctg gacgcaggat tacattgcca ttatgggcgg
     7981 tcgctaactt cgctggggtg ttttatgacg tacgcagtgg agtttgacaa cgtctcacgg
     8041 ttgtacggtg acgtgcgcgc agtagatggc gtcagtattg cgataaaaga tggtgagttc
     8101 ttctctatgc tggggccgtc cggctccggc aaaaccactt gcctgcgcct gattgctgga
     8161 ttcgaacagc tttccggcgg ggctatctct atttttggta cgcccgccag caatctgcca
     8221 ccgtgggagc gggacgtgaa tactgtcttc caggactacg cgctatttcc gcatatgtcg
     8281 attcttgaca acgtagccta cgggctgatg gtcaaaggcg tgaataaaaa gcagcggcat
     8341 gcaatggcgc aagaggcgct tgagaaagtg gcgttggggt ttgtacagca acgtaaaccg
     8401 tcacaacttt ctggtggtca gcgccagcgg gttgctatcg ccagagcact ggtgaacgaa
     8461 ccgcgtgtgt tgttgctgga tgaaccgctc ggcgcgctgg atctcaaatt gcgagagcaa
     8521 atgcagcttg agctgaaaaa gctgcaacag tctctcggta tcacttttat cttcgttacc
     8581 cacgatcagg gcgaagcgtt atcgatgtcc gatcgggtgg cggttttcaa taatggacgc
     8641 attgaacagg tcgattctcc gcgcgatctc tatatgcgcc cgcgcacgcc gtttgttgcg
     8701 ggattcgtcg ggacatccaa cgtttttgat ccgctaatgg ccgagaaact ttgtggcatg
     8761 acgggaagct tcgcactacg accggaacat atccgcctca acacccatgg cgaaatgcag
     8821 gctaatggca cgatccaggc ggtgcaatat cagggcgcag cgacccgttt tgaactgaaa
     8881 ttgaacggcg gtgaaaaact gctggtgagt caggccaata tgacaggcga agagctacct
     8941 gccacgctca cgcccggaca acaggtgatg gtttcctggt cacgtgatgt gatggtggcg
     9001 ctggttgagg agaggtgaat ggcgatgaac gtattgcaat caccttcacg tccaggtctg
     9061 ggtaaggtgt ccggtttctt ctggcgtaat ccggggctgg ggctgttttt gctgctgctt
     9121 ggcccgctaa tgtggtttgg cattgtctat ttcggctcgc tgctgacgct gttatggcag
     9181 ggcttttata cctttgacga tttcaccatg tcggtaacgc cggaactgac gctggcgaat
     9241 atccgcgcgc tgtttaatcc ggcgaattac gacatcattg tccgcacgct gaccatggct
     9301 gtggcagtca ctatcgccag cgccattctg gcttttccaa tggcgtggta tatggcgcgc
     9361 tataccagcg ggaaaatgaa agcgtttttt tatatcgcgg taatgttgcc gatgtgggcg
     9421 agctacattg ttaaagccta tgcctggacg ttattgctgg caaaagatgg cgtggcgcag
     9481 tggtttttac aacatctggg gctggaacca ctgctgactg cgttcctcac gctaccggcg
     9541 gtgggaggta atacgctgtc aacttccggg ctggggcgct ttctggtgtt tctctatatc
     9601 tggttgccgt tcatgatcct cccggtgcag gcggcgcttg agcgtttgcc gccgtcattg
     9661 ttgcaggcgt cggctgatct tggcgcacgt ccacgacaaa cctttcgcta tgtggtgctg
     9721 ccgctggcaa tcccgggtat tgccgctggc tctatcttta ccttctcact cacgctgggc
     9781 gattttattg tcccgcagct ggttggccct ccaggatatt ttatcggcaa tatggtttat
     9841 tcccagcagg gggcgattgg caatatgccg atggcggcgg cattcaccct ggtgccgatt
     9901 gttctcatcg cactttacct ggcgttcgtg aaacgtctgg gagcgttcga tgcactctga
     9961 acgcgcaccg tttttcctca aactggcggc ctggggcggc gtggttttcc tacattttcc
    10021 catcctgatt atcgctgcct atgcgtttaa cactgaagat gcggcgttta gttttccacc
    10081 gcaaggcctg acgctgcgct ggtttagcgt ggcagcacag cgtagtgata tccttgatgc
    10141 cgtgacactg tcacttaaag tggcggcgct ggcgacatta atcgcgctgg tgttagggac
    10201 gctggcagct gccgcgctgt ggcgacgaga ctttttcggc aaaaacgcca tttcgctgtt
    10261 actgttgctg cccattgcgc tgccgggcat tgtcactggt ctggcgctat taaccgcctt
    10321 taaaaccatc aatctggagc caggattttt caccatcgtg gtcggtcatg cgactttttg
    10381 cgtagtggtg gtgtttaaca atgtcatcgc ccgttttcgc cgcacctcgt ggagtctggt
    10441 tgaggcgtca atggatcttg gggccaatgg ctggcaaacc ttccgctacg tagtgttgcc
    10501 gaatctcagt tcggcgttac tggctggagg aatgctggcg tttgccttgt cgttcgatga
    10561 aatcatcgtg acgactttta cggcaggtca tgaacgaacg ttaccgttgt ggttgctcaa
    10621 tcagcttggg cgaccgcgtg atgtaccggt aaccaacgtg gtggcactgc tggttatgtt
    10681 ggtaacaacc ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg acaatggtca
    10741 ataaccactg atacaggaat atgctatgca acataagtta ctgattaacg gagaactggt
    10801 tagcggcgaa ggggaaaaac agcctgtcta taatccggca acgggggacg ttttactgga
    10861 gattgccgag gcatccgcag agcaggtcaa tgctgctgtg cgcgcggcag atgcagcatt
    10921 tgccgaatgg gggcaaacca cgccaaaagc gcgtgcggaa tgcctgctga aactggctga
    10981 tgttatcgaa gaaaatggtc aggtttttgc cgaactggag tcccgtaatt gtggcaaacc
    11041 gctgcatagt gcgttcaatg atgaaattcc ggcgattgtc gatgtctttc gctttttcgc
    11101 cggtgcggcg cgctgcctga atggtctggc ggcaggtgaa tatcttgaag gtcatacttc
    11161 gatgatccgt cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga attatccgct
    11221 gatgatggct gcgtggaaac tggctccggc gctggcggca gggaactgcg tagtgcttaa
    11281 accatcagaa attaccccgc tgaccgcgtt gaagttggca gagctggcga aagatatctt
    11341 cccggctggc gtgattaacg tgctgtttgg cagaggcaaa acggtgggcg atccgctgac
    11401 cggacatccc aaagtgcgga tggtgtcgct gacgggttct atcgccactg gggaacacat
    11461 catcagccat accgcgccgt ccattaagcg tactcatatg gaacttggtg gcaaagcgcc
    11521 agtgattgtt tttgatgatg cggatattga agctgtggtc gaaggtgtac gtacttttgg
    11581 ctattacaat gccggacagg attgtactgc ggcttgtcgg atctacgcgc aaaaaggcat
    11641 ttacgatacg ctggtggaaa aactcggtgc tgcggtggca acgttaaaat cgggttcgcc
    11701 ggatgacgag tctacggagc ttggaccttt aagctcgctg gcgcatctcg aacgcgtcag
    11761 caaggcggta gaagaggcga aagcgacagg gcacatcaaa gtgatcactg gcggtgaaaa
    11821 gcgcaagggt aatggctatt actatgcgcc gacgctgctg gctggcgcat tacaggatga
    11881 tgccatcgtg caaaaagagg tatttggtcc ggtagtgagt gttacgctct tcgacagcga
    11941 agaacaggtg gtgaactggg cgaatgacag tcagtacgga cttgcatctt cggtatggac
    12001 gaaagatgtg ggcagggcgc atcgcgtcag cgcgcggctg caatatggtt gtacctgggt
    12061 caatacccat ttcatgctgg taagtgaaat gccgcacggt gggcagaaac tttctggtta
    12121 cggcaaggat atgtcacttt atgggctgga ggattacacc gtcgtccgcc acgtcatggt
    12181 taaacattaa ggataatatt gcagatcgta agagtatgag atgatcttgc gtactgtcag
    12241 agcactgatt tctctgacag tacgtgctgc gtgcaaaatc actacatcaa ataaaccagc
    12301 caaatctcat agagataaga gtaaacaaac caacagcagc aaagacattt atcagtacta
    12361 cagttctact ggaaacattc atttttgcca ccttttatca ttgcccctat gtgtatagta
    12421 cgggttaaga aaatccgcaa taatactcat tctgaagatg gataagggca agttgctgtt
    12481 tgatgatttt ttctcaaact ggtgaaaaaa acttgatgca cgtcaaaaaa tgacgcatat
    12541 ttgcgcgcgt tttattcatc tggctggacg cccgtacatg tctctctatc aacacatgct
    12601 tgttttctat gcggttatgg cagcaatcgc atttcttatc acctggtttc tttctcatga
    12661 taagaaacgc atccgtctct taagcgcctt tctggtgggg gcaacatggc cgatgagttt
    12721 cccggtggcg ctgttgtttt ctctgtttta aatcttatgc actggcttga cggcaccacg
    12781 cagacttata tcatttggat gaatcgataa atttcacaag tggctaagga gaaagtatgt
    12841 cgcatctgga tgaagtcatc gcgcgcgtgg atgccgcgat tgaagagagc gtcattgccc
    12901 atatgaacga attattaatc gccctgagcg atgacgcgga gttaagtcgg gaagatcgct
    12961 acaccctgca gcagcgtttg cgcaccgcta ttgctcatca cggtcgcaaa cataaagaag
    13021 atatggaagc gcgccacgaa cagttaacca aaggcggtac catcctctga ttaaaatgag
    13081 cgtctggcca ccagccaggc gccaaccaca agtaaaattg cgccacaaat tgggccgatc
    13141 aacgcccgaa gtggtacgcc atggctgcgg aagatatcca gcaccagacc gccaatcagc
    13201 tggctggcga ccagtactgc aatagttgtc gccgctccca cattctgata tccgctgata
    13261 ctggcgaaga caaaaaacga tcccagcaag ccaggtataa gtgtccacca gcgcacgctg
    13321 gacaccagtt cgccaaaccc cgccatgcct tgtttaaacc ataaaataga aacaaagagc
    13381 acaatcccca ccagtgagtt cagcaacatg gcgatgagaa tggtagagga ggtctgggtg
    13441 atgcgcacca tcagcgtgtt ctggactacc agaccaatcc cggctgcaat caaaaaggca
    13501 agggtaagtg actgattcat ccaatcgcgt ccggttcagt gcgctcgtcg agttgcaact
    13561 gcataaatgt cagatccagc cagcgcccaa atttagtgcc tacctgcggc atttgcgcgg
    13621 tgacgacaaa tcccagcgac tggtgcagat gcagcgaggc ctgattttgc gattcgatcc
    13681 cggcgaccat gacatgcttc ccgcaatccc gcgcttcatc aattaatcgg cttaacaatt
    13741 tacgccccag acctttcccc tgatgatcgg gatggacata aaccgaatgt tccacggtat
    13801 ggcgaaaacc atcaaaacta cgccagtcgc caaacgaggc atatcccgtc actacgccat
    13861 cttcttcact caccagcacc ggataacctg ctatggtccg cgcttcaaac caggcaatgc
    13921 ggttatccgc atccaccgtt tggtcattcc agatagccgc cgtatacaac acggcgtggt
    13981 tataaatttc cgcaattgca gcacagtcgg ctttgcgggc aaaacggatg gacatgtctg
    14041 gcctcgataa aataatgatg atgatgttat caatactatc gaaaacagcc cgaagcaacg
    14101 gattcctcta tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
    14161 atcctgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
    14221 gcaacaaacg cagcgtaacc gatgttgggt tctgtcctcg cgtccgcatg gcgcgccggt
    14281 tccttacgtc tggtttcggt aaatgcgctc tttgtcgcac cagcgcaagc atttacagtt
    14341 ccggaagtgg gaaaacgtca attaaatgaa atcaatcgga tccgctacga actggagtta
    14401 atggcagttg ctctggctgt cgaaaacctc actatgcaaa aaaacgttgc tatgttgtgc
    14461 cagcaataca gccgataaat aaaatacgcc cggcaaatac cgggcttaaa gataattact
    14521 caaatcgcca cgcaatattt atgccgacac cataatttcg tccgggcgca ggttcgtaat
    14581 atcgcccgtt tgactcattg acaatgacag aaccaacgta ttctttatcg aataaattat
    14641 cgacacgacc aaataaatcc acggttaaat tgtggtaatt gtatttatac ccggtgaata
    14701 agccgacgag tgtataagaa ggcgctttgg ccgtattttc atcatctgcc ataatatcgc
    14761 ccatataacg cgcttccgtg cctgcatacc aaccatcttc cggtatataa cctatcgacg
    14821 caaagcccat attacgggcg atccctggca tccggttacc gttacaatcc tgttcattgc
    14881 agacattgct gcgataggtc gcatcaagcc aggtccagga cgcttttacg cgaaaatctc
    14941 ccgcgaaacg ttgatcccat gccagttcag cgccttgacg acgggtcttt ccggcatttt
    15001 tgtaagtcgt acgcccaccg ctactgctat cgacaacaat ttcatcatcg gtgtcggtct
    15061 ggaacaatgc gagactaagc agtccatcac caatacgcgt tttactgccg atctcaattg
    15121 tatcgttggt ggagggtttt aaaccaaagt tcataccgct ttgtccatca gcgcgataag
    15181 acagttcatt aatcgtcggc gtttcaaaac cgcgcccggc tgccagatag acattccagg
    15241 catcggtcat tgcatatttt aacgatccgg caggtagcca tttgtgataa ctggcatcac
    15301 cgctgtcatc gccgttaccc ggagtaacgt aatgatcgtt ggaatcaaac cacacggagc
    15361 tgtagcgcac gccagcatcc agcgaaagtt tgtcggtcag ttgccactgg gtttgtaaat
    15421 agggatcgac gttccacatc aaattgcgtt cgtcgcggcg caactcacct ttttgcccgt
    15481 attccggcac gccgcgattc aggcggaagt tattgtaacc cttgcggttt tcactcatgt
    15541 tttcgtagtt caggccggtt gtgaacgtga ctggaacgcc cagttcacca cggtgtgtcc
    15601 agcggctgtc tatcccctgg taatggcgtt gcagggtaat cacgccgccc gcatgtgacg
    15661 ggttaagttg cggggccatg gggattgact ggtactgggt cgtttctcgc tctccggcat
    15721 acatcatcac actcatatca tcttgtgcgc tcaggctacg ctcatagcgc aacccagcct
    15781 gagtttgctt gatggttttt cgcatgtcgt actgttctgc acgaggcgct tgttgtggat
    15841 tcgccttcca ttctgcttcg gttagcccac ctgggtcatc tgctttgata tccacactat
    15901 tgaaaatcag gcttaatttg ctggcgtcat caatgcgtac gcccagtttg gcattggcta
    15961 aatttttctg tgcgccacta tggtcacgat agccgtgggt cgtaaaacgt gtggttgcga
    16021 cggtgtaatc gacatcgccg ggttgtgtgc cgtcgcccgt tgcgcccgtt gctttcagcc
    16081 catagcgcca gctgccaaaa ctgccgtagt aactactggc ttcaatggtt ggtggctgtt
    16141 gtccggtctg ggtggtgaca ttcattacac cgccagacgc gttgccatac agagcagaga
    16201 aggggccacg cagcacttcc acattttgca cactgcttaa atcgatgtta gatgtctgcc
    16261 cttgtccgtc gggcatggta gcgggaatac cgtccacata caggcgaata ccgcgaattc
    16321 cgtaagtgga gcgggagcca aatccgcgaa tcgacagctg taaatcttgc gcatagttct
    16381 gtcggttttg tacctgtaaa ccaggcactc cggtcagtga ttcagacaag ttaatgcgcg
    16441 gtgttgccag gcgcatctcc tcgccatcca ccacgctcac ggctgctggg gtatccagtt
    16501 ctgaaaccac ctgcggtgcg gcactgacaa tcatggtctg ttcatcagcg gcaaaaacaa
    16561 cgggggaaag gacaagcagt gcgggcaaaa cggtctgtcg gacggaaaaa atcttcatga
    16621 aaaaagccag gttaagaatg ggaaaacacc gtcatggtaa taaaattgta aatttatgga
    16681 aaacaaaacg gcacaatacg ttaagtaatt gagaaaattg tagtcataac ggtaagaaat
    16741 gctccacatt tgagaaaata atgattacca ttcccattta taacaagggc gtaacgatga
    16801 ttacgcttag cgaagcattg tgaagcagca aaaatatcgg ttcatcaaag ggagtcgtca
    16861 tgcatttacg tcatctgttt tcatcgcgcc tgcgtggttc attactgtta ggttcattgc
    16921 tcgttgcttc atcattcagt acgcaggccg cagaagaaat gctgcgtaaa gcggtaggta
    16981 aaggtgccta cgaaatggct tatagccagc aagaaaacgc gctgtggatt gccacttcgc
    17041 aaagccgcaa actggataaa ggcggcgtgg tttatcgtct tgatccggtt actctggaag
    17101 tgacgcaggc gatccataac gatctcaagc cgtttggtgc caccatcaat aacacgactc
    17161 agacgttgtg gtttggtaac accgtaaata gtgcggtcac ggcgatagat gccaaaacgg
    17221 gcgaagtgaa aggacgtctg gtgctggatg atcgtaagcg cacggaagag gtacgcccgt
    17281 tgcaaccacg tgagctggta gctgacgata ccacgaacac cgtttacatc agtggtattg
    17341 gtaaagagag cgtgatttgg gtcgttgatg gcgagaatat caaactgaaa accgccatcc
    17401 agaacaccgg taaaatgagt accggtctgg cgctggatag caaaggcaaa cgtctttaca
    17461 ccactaacgc tgacggcgaa ttgattacca tcgacaccac cgacaataaa atcctcagcc
    17521 gtaaaaagct gctggatgac ggcaaagagc acttctttat caacatcagc cttgatacca
    17581 ccaatcagcg tgcatttatc accgattcta aagcggcaga agtgttagtg gtcgataccc
    17641 gtaatggcaa tattctggcg aaggttgcgg caccggaatc actggctgtg ctgtttaacc
    17701 cagcgcgtaa tgaagcctac gtgacgcatc gtcaggcagg taaagtcagt gtgattgacg
    17761 cgaaaagcta taaagtggtg aaaacgttcg atacgccgac tcatccgaac agtctggcgc
    17821 tgtctgccga tggcaaaacg ctgtatgtca gtgtgaaaca aaaatccact aaacagcagg
    17881 aagctaccca gccggacgat gtgattcgta ttgcgctgta atagatatgg cgagggtgaa
    17941 aacggaaaga attgcctgac ttttatcacg ccgcatccgg caccttcgtc agtagtctga
    18001 atttgcccgg cacgtagccg ggcaattttg caattaactc gccgtttcag gcttaaacac
    18061 aatgtcctgt ttttcttcat cttcctcgac gactggcgca gtgctgtgaa tttcagcaac
    18121 gcgtttgcgc acaccaaacc agcctataac cagcagaata ccgataatcg gcagcgccgc
    18181 gatggtataa gtcccgttcg ggtaatcgaa cgccatcagc accaggacac tcagtaaaaa
    18241 cagtaatgtc agccaggaag tgaagggcgc gccaggcagt ttaaaactga catccgctgc
    18301 tttgccttct ttaatcgctt tacgcaggcg catctggcaa acgatgataa acgcccatga
    18361 agcgatgatt cccagcgacg cgaagttcaa cacaatctca aatacgcgtg acggcaccag
    18421 atagttgagg aatacgccga cgacatacac aaccagtgtc gccagaatcc cggcatacgg
    18481 cacatgctga cgactcattt tcgccataaa actcggtgcg gaaccgccca tcgccatcga
    18541 gcgcagaata cgcccggtgc agtacagacc tgaattcagg ctggagaggg cagcggtcag
    18601 caccacaatg ttcataatgc tgcctatata tggcacaccc agtttagaga aaaacgtcac
    18661 gaacggactt tgccccgcct gatatgcgct ccacggcaat aacataacca gcaacaccac
    18721 ggagccgacg taaaacagac caatacgcca aatcacactg ttaatcgctt taggtaccat
    18781 ggtctgcgga tctttacatt ctccggccgc tgtacccacc atttcaatgg aggcaaaagc
    18841 aaacactacg ccctgaatca acaccagcgc aggcagcaaa ccgtggggga agaagccgcc
    18901 attatcggtg attaaatgaa agccagtggt gttgccatcc agcggctgac cgctaccgag
    18961 gaacactgta cccacgacca aaaaggttac aatggcgagc actttaataa gcgcaaacca
    19021 gaactccatc tccgcaaacc atttcacacc gatcatattc atggtgccaa cgatggtcag
    19081 tgcagcgagc gcaaagaccc actgcggcac gccgccaaac gcgccccagt aatgcatata
    19141 cagagcgacg gcggtaatat caacaatccc cgtcatcgcc cagttgatga agtacatcca
    19201 gccagcaaca taagcggcct tctcacccaa aaactcacgg gcataagaga caaaactgcc
    19261 actggaaggg cggtgtaaca ccagctcacc caatgcacgc aaaataaaaa acgaaaacag
    19321 gccgcagatc aaatagacca gtgccagtgc gggccccgcc atttgcagtc gcgcccccgc
    19381 gcctaaaaac aaacccgtac caatcgcgcc gccaatggcg atcatctgta cctggcgatt
    19441 gcccatcgct ttgtgatacc cctcttcgtg ggcattaagc cagcggcgtt tcgcggcgtg
    19501 ttgatctgaa gtgtcggtgt tgtgtttact cattgctctc cctgattgct ttaatgaaaa
    19561 agtcatataa gttgccatga acaatgttta ttcatcctgg cgatgacaag attcaccatt
    19621 attctttatg ggtataactg tcggcgcaga atcctacctt ttatcatgta acgatgcaaa
    19681 ataatcccgc tataaatatg tggggataaa gtcggctggc gacaaaatag caacctgatg
    19741 aaattatgac ggaaacaacc actattggtt tgatgatacc tgactgcagt cctactcttg
    19801 cgccactcgt tcgtcgctct gaacgccagt ggttgcagtg gacactggcg tggttggctc
    19861 aatgaatagt gaggggctag cacatgatgg gtttcagggg taatacgcca aataccaata
    19921 caattgctga ttaaatgagc caattgccga cattacagaa aaggctaaaa aatgtaagag
    19981 gcatgtaggg tgataaacgc gctattgccc aacaggagtt tctggaatta gtcctgttcc
    20041 atgggcatt
//
LOCUS       DAPQZD010000081        19471 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPQZD010000081 DAPQZD010000000
VERSION     DAPQZD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.081"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..448
                     /locus_tag="RO454_002827"
     CDS             <1..448
                     /locus_tag="RO454_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDY0934633.1"
                     /translation="AFDYLSSPGSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGK
                     PLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHA
                     QYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGLSAH"
     gene            588..923
                     /locus_tag="RO454_002828"
                     /pseudo
     CDS             588..923
                     /locus_tag="RO454_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(993..1475)
                     /locus_tag="RO454_002829"
     CDS             complement(993..1475)
                     /locus_tag="RO454_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDY0934634.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1710..4292
                     /gene="leuS"
                     /locus_tag="RO454_002830"
     CDS             1710..4292
                     /gene="leuS"
                     /locus_tag="RO454_002830"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDY0934635.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4307..4888
                     /gene="lptE"
                     /locus_tag="RO454_002831"
     CDS             4307..4888
                     /gene="lptE"
                     /locus_tag="RO454_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDY0934636.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4888..5919
                     /gene="holA"
                     /locus_tag="RO454_002832"
     CDS             4888..5919
                     /gene="holA"
                     /locus_tag="RO454_002832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDY0934637.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5921..6562
                     /gene="nadD"
                     /locus_tag="RO454_002833"
     CDS             5921..6562
                     /gene="nadD"
                     /locus_tag="RO454_002833"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDY0934638.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6586..7197
                     /locus_tag="RO454_002834"
     CDS             6586..7197
                     /locus_tag="RO454_002834"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDY0934639.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7457..7774
                     /gene="rsfS"
                     /locus_tag="RO454_002835"
                     /gene_synonym="rsfA"
     CDS             7457..7774
                     /gene="rsfS"
                     /locus_tag="RO454_002835"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDY0934640.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7778..8245
                     /gene="rlmH"
                     /locus_tag="RO454_002836"
     CDS             7778..8245
                     /gene="rlmH"
                     /locus_tag="RO454_002836"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDY0934641.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8276..10177
                     /gene="mrdA"
                     /locus_tag="RO454_002837"
     CDS             8276..10177
                     /gene="mrdA"
                     /locus_tag="RO454_002837"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDY0934642.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10180..11292
                     /gene="mrdB"
                     /locus_tag="RO454_002838"
                     /gene_synonym="rodA"
     CDS             10180..11292
                     /gene="mrdB"
                     /locus_tag="RO454_002838"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDY0934643.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11303..12391
                     /gene="rlpA"
                     /locus_tag="RO454_002839"
     CDS             11303..12391
                     /gene="rlpA"
                     /locus_tag="RO454_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDY0934644.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12531..13742
                     /gene="dacA"
                     /locus_tag="RO454_002840"
     CDS             12531..13742
                     /gene="dacA"
                     /locus_tag="RO454_002840"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDY0934645.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13852..14115
                     /locus_tag="RO454_002841"
     CDS             13852..14115
                     /locus_tag="RO454_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDY0934646.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14216..14857
                     /gene="lipB"
                     /locus_tag="RO454_002842"
     CDS             14216..14857
                     /gene="lipB"
                     /locus_tag="RO454_002842"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDY0934647.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15117..15641
                     /locus_tag="RO454_002843"
                     /pseudo
     CDS             15117..15641
                     /locus_tag="RO454_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15882..16847
                     /gene="lipA"
                     /locus_tag="RO454_002844"
     CDS             15882..16847
                     /gene="lipA"
                     /locus_tag="RO454_002844"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDY0934648.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(16947..17150)
                     /gene="tatE"
                     /locus_tag="RO454_002845"
     CDS             complement(16947..17150)
                     /gene="tatE"
                     /locus_tag="RO454_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDY0934649.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17279..18067)
                     /locus_tag="RO454_002846"
     CDS             complement(17279..18067)
                     /locus_tag="RO454_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDY0934650.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18160..18543
                     /gene="crcB"
                     /locus_tag="RO454_002847"
     CDS             18160..18543
                     /gene="crcB"
                     /locus_tag="RO454_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDY0934651.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(18597..18806)
                     /gene="cspE"
                     /locus_tag="RO454_002848"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(18597..18806)
                     /gene="cspE"
                     /locus_tag="RO454_002848"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDY0934652.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(18981..>19471)
                     /gene="pagP"
                     /locus_tag="RO454_002849"
     CDS             complement(18981..>19471)
                     /gene="pagP"
                     /locus_tag="RO454_002849"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDY0934653.1"
                     /translation="ANADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKT
                     DRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENF
                     HLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYFAWMRF
                     QF"
BASE COUNT         4871 a         4973 c         4905 g         4722 t
ORIGIN      
        1 tgcctttgat tatttaagct caccgggcag tcttccattc accacggcag ccacggagct
       61 ttccgcgatt catggtcact caacgtcgca atatcgcctt ggagaatttt atcttcatgg
      121 tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc aatcagcaga
      181 acaggaaaat ccacgcgcgc aaagtaaact ggggtggatc tacctcaaag gtctgggggt
      241 caaacccgac acccgtaaag caattctctg gtataaggaa gcagctgaac aagggtatgc
      301 tcatgctcaa tatactttgg gtttgatcta cagaaatggc tcaggtatta atgttaacca
      361 ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa atgcggaaag
      421 attacttgcc gggctttccg cacattaacg tcgactgtca cccgaacaga tggagtattt
      481 tccctatcaa accagcaaag ataaaaatat agaaaataat tttaatgatg cccagacagc
      541 ctggcccacc atcgtcaacg ggcattggct gcggttgcgg ctccgataac gctggcgaag
      601 gggtggacca ggactataag caagcgatgt actggtatct gaaggctgct gctcaggaaa
      661 gcgtcggcgc ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag
      721 attatcaggc cgcctttgaa tagtttacga tagccgctga atgcaatgac gccacagcct
      781 ggtataacct ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg
      841 ctctcgactt gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caagaaattc
      901 gtgagattga agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca
      961 tattctcttt attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac
     1021 tggtcatgac cacatttagg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg
     1081 aagtgacatt tctcgcagac cagatttccc agccctacca cttctccgct gtgataaacc
     1141 ccatgatgat tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgatg
     1201 tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct gtcagactct
     1261 tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc
     1321 gtcagctcat cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc
     1381 tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg
     1441 accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact
     1501 gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa
     1561 attaatgtat gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga
     1621 cgcgggcgtt tgggctatgc tatgcggatc tgaaaaacca catcaacgct acatttgtag
     1681 ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag
     1741 aatccaaagt acagcttcac tgggatgaga agcgcacatt tgaagtaacc gaagacgaga
     1801 gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg
     1861 gccacgttcg caactacacc atcggtgacg tgatcgcccg ctaccagcgc atgctgggca
     1921 aaaacgttct gcagccaatc ggctgggacg catttggtct gcctgcggaa ggcgcggcgg
     1981 tgaaaaacaa caccgcgcca gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc
     2041 agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcgacc tgtacgccgg
     2101 aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata
     2161 agaagacttc tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag
     2221 ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga gcgtaaagag atcccgcagt
     2281 ggtttatcaa aatcacagct tacgctgacg agctgctcaa cgatctggat aaactggatc
     2341 actggccaga caccgttaaa acaatgcagc gtaactggat cggtcgttcc gaaggcgtgg
     2401 aaatcacctt caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg
     2461 acacctttat gggttgtacc tacctggcgg tggctgcggg tcatccgctg gcacagaaag
     2521 cggcggaaaa taatcctgaa ctggccgcct ttattgacga atgccgtaac accaaagttg
     2581 ccgaagctga aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagcggttc
     2641 acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggaatacg
     2701 gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta
     2761 aatacggcct gaacatcaaa ccggttatcc tggcagctga cggctctgag ccagatcttt
     2821 ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc tggcgagttt aatggtcttg
     2881 accatgaagc ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc
     2941 gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc
     3001 cgattccgat ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc
     3061 cggtgatcct gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc
     3121 cggagtgggc aaaaactacc gttaacggta tgccagcact gcgtgaaacc gacactttcg
     3181 acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccggag tacaaagaag
     3241 gtatgctgga ttccaaagcg gctaactatt ggctgccggt ggatatttac attggtggta
     3301 tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg
     3361 caggcatggt gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg
     3421 cagatgcctt ctactatgtt ggtgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg
     3481 ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg
     3541 aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc
     3601 aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc
     3661 cggctgatat gactctcgaa tggcaggaat ccggcgtgga aggggctaac cgcttcctga
     3721 aacgtgtctg gaaactggtg tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg
     3781 tcgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta
     3841 aagtaaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg
     3901 agctgatgaa caaactggcg aaagcaccga ccgatggcga gcaggatcgc gccctgatgc
     3961 aggaagcgct gctggcggta gtccgtatgc ttaacccgtt caccccgcac atctgcttca
     4021 cgctgtggca ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg
     4081 acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc
     4141 gtgcaaaaat caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc
     4201 aggaacatct ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg atttacgtac
     4261 caggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc
     4321 aacattgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga
     4381 caccacgcag gttccttcca ctatgaaagt catgatcctg gactcaggcg atccgaacgg
     4441 gccattaagc cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa
     4501 agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga
     4561 taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa
     4621 tgcgaccgtg ttgatccccg gccgtgacat ctacccgatt agcgccaagg tcttccgttc
     4681 gttcttcgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa
     4741 agagatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc
     4801 agatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc
     4861 acgcgtctcc accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg
     4921 cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg
     4981 caggaaagcc aggacgctgt tcgtcaggtc gctgcggcgc aagggttcga agaacaccac
     5041 actttttcca ttgatcccaa caccgactgg aatgcgattt tttcgttatg ccaggccatg
     5101 agcctgtttg ccagtcgaca aacgctattg ctgttgttac ccgaaaacgg accgaatgcg
     5161 gcgatcaatg aacaacttct cacactcacc ggacttctgc atgacgatct actgttgatc
     5221 gtccgcggta ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg
     5281 aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagctgcc ccgctgggtt
     5341 gctgcgcgcg caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc
     5401 tactgttatg aaggtaacct gctggcgctg gcccaggcac tggagcgttt atcgctactc
     5461 tggccggacg gcaaactgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat
     5521 ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat
     5581 attcttcagc aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg cacattacaa
     5641 cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg
     5701 ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgagcga agcgttaaat
     5761 cgcttaagcc agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc
     5821 ctcaaacaag attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg
     5881 tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct
     5941 gtttggcggc acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc
     6001 gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc
     6061 ccagccggaa gcgaacagca tgcagcgtaa acacatgctt gaactggcga ttgccgacaa
     6121 gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca
     6181 aacactgaaa gagtggcggc aggaacaagg accggacgtg ccgctggcgt ttattattgg
     6241 tcaggattca ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc
     6301 acatttgatc gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca
     6361 gcaatggctg gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg
     6421 taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg
     6481 tttgcaaaac ggtgaatcgt gtgaggattt attaccggaa ccggtactga cttacattaa
     6541 ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta
     6601 attcgtcatg gtgaaacgca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca
     6661 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatgatgtt
     6721 tcctttgacc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     6781 cttagtgacc gccagcaccc cgttcatatc atccctgaac tcaatgaaat gttttttggc
     6841 gactgggaga tgcgacatca tcgtgacctc atgcaagaag atgccgaaaa ctatagcgcg
     6901 tggtgcaatg actggcagca agctatcccc acgaacggtg aaggatttca ggcattttcg
     6961 caacgtgtgg aacgatttat cgcaaggctt agtgaatatc agcactatca gaatatttta
     7021 gtcgtcagcc atcagggcgt actgagtctg ttaattgctc gtttaattgg tatgcctgcc
     7081 gagtctatgt ggcatttccg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     7141 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt
     7201 ttctgttttt ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc
     7261 tccgcagcca gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc
     7321 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
     7381 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
     7441 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
     7501 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca gcatcaccga
     7561 ctgcatgatc atctgtacgg gtacgtccag ccgccatgtt atgtccattg ctgatcacgt
     7621 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
     7681 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
     7741 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
     7801 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
     7861 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
     7921 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
     7981 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
     8041 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
     8101 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcac ttacccttcc
     8161 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
     8221 caaccatcct tatcaccgtg agtgataagt gagctttgag tagaaaacgc agcggatgaa
     8281 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
     8341 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
     8401 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
     8461 gcctatcgca cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggctctcaa
     8521 ccgcactatc taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct
     8581 ggacgctttg cgcagcgtgg ttgatctgac cgatgatgat attgctgcat tccgaaaaga
     8641 gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa actaacctga ccgaagtaca
     8701 agtagctcgc tttgccgtca accagtaccg ttttccgggt gttgaagtta aaggctataa
     8761 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
     8821 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
     8881 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
     8941 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
     9001 accaccgcag gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
     9061 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtaccggtgg
     9121 ggtgttggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc
     9181 cagcaaagat tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac
     9241 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
     9301 cgccggagtg atcacgcgca atacgacgtt gtttgacccc ggctggtggc aacttccagg
     9361 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
     9421 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
     9481 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
     9541 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
     9601 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
     9661 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
     9721 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
     9781 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
     9841 tgctaaccgc cctaacggta cggcgcataa atattttgcc agcgcaccgt acaaaattgc
     9901 ggcgaaatcc ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
     9961 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    10021 tccgcaagtg gctgtcgcca tgattctgga gaacggtggc gcgggtccgg cggttggtac
    10081 gctgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    10141 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    10201 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    10261 ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag
    10321 cgtaaaatcg gccagattgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    10381 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    10441 gcggtagatg ctttcggtgc catctctaaa ggcgctcaac gctggctgga cctcggtatt
    10501 gttcgttttc aaccgtcgga aattgccaag atagccgtac cgttgatggt tgcacgcttt
    10561 atcaaccgcg acgtttgccc accttcattg aagaatacag ctatcgcgct ggtgctgata
    10621 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    10681 ctttccggcc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    10741 gtagtgctgg tagcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag
    10801 cgccagcgcg taatgatgct cctggacccg gaatcagacc cactcggcgc aggctatcac
    10861 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac
    10921 ggcactcagt cacagcttga atttctcccc gaacgccata ccgactttat cttcgcggta
    10981 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    11041 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    11101 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    11161 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt
    11221 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    11281 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    11341 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    11401 tgcggtatgt aacggcccca tagttgaaat tagcggggca gacccgcgtt tcgaaccact
    11461 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    11521 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac ccggcagtaa
    11581 cctgacggcc tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct
    11641 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    11701 cattaatgat cgcggccctt atggcaacga ccgcgtgatt tcgctttctc gcgcggcagc
    11761 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    11821 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    11881 cctgccagca cctcccgatt taagcggtgg cgcgggaaca agttcagtgc ctgtcccgca
    11941 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    12001 ggtaaccagc agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    12061 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    12121 ctcacctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    12181 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    12241 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    12301 cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    12361 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    12421 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    12481 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    12541 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    12601 tctctgctgc acatgccgat gacctgaata tcaaaaccat gatcccgggt gtaccgcaga
    12661 tcgacgcgga gtcctacatc ctgattgact ataactccgg caaagtactc gccgaacaga
    12721 acgctgatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    12781 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    12841 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    12901 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    12961 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    13021 gctacgttaa cgccctgggc ctgaaaaata cccacttcca gacggtacac ggtctggatg
    13081 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggcg ttgatccgtg
    13141 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    13201 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    13261 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    13321 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaat
    13381 tgctaacctg gggcttccgt ttcttcgaaa ccgttaatcc tctgaaagta ggtaaagagt
    13441 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    13501 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagctagc tatgtgctga
    13561 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    13621 agcttgacgg caaaacgatc gaacaacgcc cgctggttgt gctgcaagaa atcccggaag
    13681 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    13741 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaac
    13801 tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc
    13861 aaacttaacg aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg
    13921 ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac
    13981 accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac
    14041 gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc
    14101 cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc
    14161 ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta
    14221 tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccaggc
    14281 tatgcatgaa ttcaccgata cccgcgatga aagtaccctt gatgaaatct ggctggtcga
    14341 gcactatccg gtattcaccc aagggcaggc aggaaaggcg gagcacattt taatgccggg
    14401 tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccggggca
    14461 acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt
    14521 gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc
    14581 tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg
    14641 tattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc
    14701 atttttacgt attaatcctt gtgggtatgc aggaatggaa atggctaaaa tatcacaatg
    14761 gaaacccgaa gcgacgacta ataatattgc accacgttta ctggaaaata ttttagcgct
    14821 actaaacaat cctgacttcg aatatattac cgcttaattc catacatcaa tggcccaatt
    14881 tacactgggt cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata
    14941 ttttctttat cacttgcaga aatgcgatgc tacgccttgc tccgtagccc acctaattga
    15001 ttaactcatt gaaataattg accatggtga gcaaaagcgt tccgtcccaa ttagacataa
    15061 gattattgta atttttttaa atattatttt ttcatgaata aaattggagt aagtgcgtgg
    15121 atagtaataa taaaattgaa ccctgtttaa gcagaaaatc gtcagaaggt aaaccacaaa
    15181 tatttacaac cttacgaaat attgatctta cccttctgac tattttgaag ttgcatatgt
    15241 ccataaagga atcgttaatg cagcgaaagt gcttaatctg ccccccctcg gcaatctatc
    15301 agcctattca gaaactgcgc gttatattcc ctgacctatt gtttattcgc aaaggccagg
    15361 gtgtcactcc taccgcattt gcgatgtatc tatatgagtc tatcactcag ggacttgagt
    15421 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacggacg ataactattg
    15481 cgccctcccc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    15541 actatccaca gcttttactg cgcaacccgc ccatcagcgg cgcggaaaac caactcagtc
    15601 agtttcaaac cgatctcatc atcaataaca tgttttgcac ggggtcatag atgttatccg
    15661 taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcacaacga taaaatggca
    15721 caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa
    15781 tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg
    15841 aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga
    15901 tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg
    15961 tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag
    16021 cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg
    16081 tctgcgagga agcctcctgc cctaacctgg cggaatgctt taaccacggc actgcaacgt
    16141 ttatgatcct cggcgctatt tgtacccgcc gttgcccgtt ctgtgacgtt gcccacggtc
    16201 gcccggtagc tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg
    16261 cgctgcgtta tgtggttatc acctccgttg accgtgatga tctgcgcgat ggcggtgccc
    16321 agcactttgc ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa
    16381 ctctggtgcc ggatttccgc ggtcgtatgg atcgtgctct ggatattctg actgcaacgc
    16441 caccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc
    16501 ctggtgcaga ttacaactgg tcgcttaagc tgctggaacg ctttaaagaa gcgcatccgg
    16561 aaatcccgac caagtctggt ctgatggtgg gactgggtga aaccaatgaa gaaattattg
    16621 aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgct aacgctgggg caatatttgc
    16681 agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa
    16741 tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct
    16801 cttcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt
    16861 acttacatct gtctgataaa aaacccgctt tttgaagcgg gtttttgtat cgaacaagat
    16921 attgagggag cgtcctgctc gccacgtcac tctttatgag agagcttttc agcctgaaga
    16981 tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct
    17041 ttaatggccg ctccaaggtc tccgcccagc gtacgtaact tcttagtccc aaacagcaga
    17101 acgaccagcg ccgcaactac cagcagtttg gtaatactaa tctcacccat agataccttc
    17161 ttgacatata accggcaaca gaacgcgcct taaaaaccag agaatattaa cggtcattcg
    17221 acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc
    17281 ataataattg cggcggcgca aagcgacggt tgtttaagac gggcagttgc gcgcgcacct
    17341 gacgcacacg ttcgggcgtc acttccgcca taatgagtgc aggcatttct gacgctgccg
    17401 caatggtgac gccaaaggga tctataatcc ggctttgccc gatatttttg ttcccgcact
    17461 cccccgccgc caccatataa caggtggtat ccagcgcacg agcggcaagc aacgttgacc
    17521 agtgatgctc tttgagcggc ccacgaaccc aggcggcagg aagtaccagt atttcagctc
    17581 cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa gtcataacag gtcatcagac
    17641 cgaccttcat cccctccacc tccagtaacg gagctatttc attaccagca tcaacatggc
    17701 gtgattcctg aatggcaaat gcatcataga gatgcagttt ggcataacgg gcgacgatgt
    17761 ttcctgcctg tagcgccacc agcatattcc atgcgcgccc cggtgttgaa ggaacatgaa
    17821 tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg ccgtaaacga ccgaggaatt
    17881 cgccttccag cagttgtgct gatttaaccg atagatctgc atcatgatca tcacgcgcca
    17941 gtaatgcttc cggcaggaca aacagcgaaa cctcgttttc cgccgcctgc gccatcaacg
    18001 aggcacaaat ctcaacgttc ttttcccaca cagatgtaac agcaaactgt cctgcggcaa
    18061 ccagcattgc cacctcctgt cagagtcgta aaattcgcca acggcgatta ttcggcgtta
    18121 cacttatcac tcatacaaat caaatagcag gattttgcag tgttacaact tcttttagca
    18181 gtttttattg gcggtggtac gggaagcgtg gcgagatggc tgttaagtat gcgatttaac
    18241 ccgctgcatc aggcgattcc gttgggaacg ctggcagcaa acctgattgg ggcattcatc
    18301 ataggaatgg gattcgcctg gttcagcagg atgacgaaca ttgatccagt gtggaaagta
    18361 ttaatcacca ccggattttg tggcggtcta acaaccttct caacattttc ggcagaagtg
    18421 gtgtttttgt tacaagaggg ccgctttggc tgggcgttac tgaacgtttt cgtcaacctt
    18481 ttggggtctt ttgccatgac cgcactggct ttctggctgt tttccgcctc aaccgcacac
    18541 taaagaccaa aaaaaacccg ctgattaagc gggttttgaa ttcttgctga cgtatcttac
    18601 agagcgatta cgtttgcagc agaagggcct ttggcaccgt tagtgatttc gaactctacg
    18661 cgctgacctt cagcaagagt tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac
    18721 acgtctttgc tgccgtcttc cggagtaatg aaaccgaatc ctttggactc attaaaccac
    18781 ttaacgttac ctttaatctt agacatcaaa attaccttta catgaaaaat agacacaaat
    18841 gctgtgtcgg ttaccagtac accaattgtg gtacgctttg tccagtgcaa ctttactaaa
    18901 aagtgataaa agtcgcgtta tttttgatgt taattatttt agtggttgat tttaaagaat
    18961 ttactaaaac ttcatttgtc tcaaaactga aagcgcatcc aggcaaagta cacattgcca
    19021 ttgttgtagg tacccggaat gtaggtcatc tgaaacgtcg cggggccata ccccacagag
    19081 gccaatggca gtagaaccgg aagagggatg taattccagt tatcgcgcgc cgttacgcca
    19141 gcggtaaatc ccaggcctaa atgaaaattt tcatccgcca gcggtcgcca ggtactttcc
    19201 catccgtatc cggcaatcgg ttcccatttg ttccacgagt ccttaaatgc cattgcatac
    19261 aggccatgcc agtttccttt ttcatcccaa cgcgactgac caaaaccgcc accccacggt
    19321 cgctcgttat agcgatcggt tttttctttg tcataggcaa aacgcgcatg ccaggtgatg
    19381 gcaggaatat ataaatcata atgttctggc tgttgccagg tttgtgcaat attttctcta
    19441 aacgttgtca tccactcatc agcgttagca a
//
LOCUS       DAPQZD010000082        19171 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPQZD010000082 DAPQZD010000000
VERSION     DAPQZD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.082"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..894
                     /locus_tag="RO454_002850"
     CDS             <1..894
                     /locus_tag="RO454_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0934654.1"
                     /translation="LYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQR
                     LRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIYGP
                     QLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSV
                     ICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFIN
                     RKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLYISY
                     NKASLNNLVFSRFIDRLNDSF"
     gene            1041..2261
                     /locus_tag="RO454_002851"
     CDS             1041..2261
                     /locus_tag="RO454_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDY0934655.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            2234..2863
                     /locus_tag="RO454_002852"
     CDS             2234..2863
                     /locus_tag="RO454_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDY0934656.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(2864..4024)
                     /gene="ybdL"
                     /locus_tag="RO454_002853"
     CDS             complement(2864..4024)
                     /gene="ybdL"
                     /locus_tag="RO454_002853"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDY0934657.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTKMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4133..5221
                     /gene="hcxA"
                     /locus_tag="RO454_002854"
     CDS             4133..5221
                     /gene="hcxA"
                     /locus_tag="RO454_002854"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDY0934658.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5231..5428)
                     /locus_tag="RO454_002855"
     CDS             complement(5231..5428)
                     /locus_tag="RO454_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDY0934659.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5541..7646)
                     /gene="cstA"
                     /locus_tag="RO454_002856"
     CDS             complement(5541..7646)
                     /gene="cstA"
                     /locus_tag="RO454_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDY0934660.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7827..8240)
                     /gene="entH"
                     /locus_tag="RO454_002857"
     CDS             complement(7827..8240)
                     /gene="entH"
                     /locus_tag="RO454_002857"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDY0934661.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8243..8989)
                     /gene="entA"
                     /locus_tag="RO454_002858"
     CDS             complement(8243..8989)
                     /gene="entA"
                     /locus_tag="RO454_002858"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDY0934662.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8989..9846)
                     /gene="entB"
                     /locus_tag="RO454_002859"
     CDS             complement(8989..9846)
                     /gene="entB"
                     /locus_tag="RO454_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDY0934663.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9860..11470)
                     /gene="entE"
                     /locus_tag="RO454_002860"
     CDS             complement(9860..11470)
                     /gene="entE"
                     /locus_tag="RO454_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDY0934664.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(11480..12655)
                     /gene="entC"
                     /locus_tag="RO454_002861"
     CDS             complement(11480..12655)
                     /gene="entC"
                     /locus_tag="RO454_002861"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDY0934665.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12844..13800
                     /gene="fepB"
                     /locus_tag="RO454_002862"
     CDS             12844..13800
                     /gene="fepB"
                     /locus_tag="RO454_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDY0934666.1"
                     /translation="MRLASLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIALFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13804..15054)
                     /gene="entS"
                     /locus_tag="RO454_002863"
     CDS             complement(13804..15054)
                     /gene="entS"
                     /locus_tag="RO454_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDY0934667.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLSVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            15165..16169
                     /gene="fepD"
                     /locus_tag="RO454_002864"
     CDS             15165..16169
                     /gene="fepD"
                     /locus_tag="RO454_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDY0934668.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16166..17158
                     /gene="fepG"
                     /locus_tag="RO454_002865"
     CDS             16166..17158
                     /gene="fepG"
                     /locus_tag="RO454_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDY0934669.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17155..17970
                     /gene="fepC"
                     /locus_tag="RO454_002866"
     CDS             17155..17970
                     /gene="fepC"
                     /locus_tag="RO454_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDY0934670.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17967..19100)
                     /gene="fepE"
                     /locus_tag="RO454_002867"
     CDS             complement(17967..19100)
                     /gene="fepE"
                     /locus_tag="RO454_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDY0934671.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GQGKAIIVILSALVGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4639 a         5344 c         4802 g         4386 t
ORIGIN      
        1 ctttatgatc taaaaaaatt tgacctcaat ttactggtta tatttgaatg tatttatcaa
       61 catctcagca ttagtaaagc ggcagaatca ttatatataa caccctctgc tgttagtcag
      121 tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc agggaaaggt
      181 atcgcgccga ctacaacagg gttaaacctg catcaccatc ttgagaaaaa tctcagaggc
      241 cttgaacaaa cgatcaatat cgttaataag tctgaactaa agaaaaattt tattatctac
      301 ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct tagacaagat
      361 acttcaatcg atatagaatg ccatgacata ctgctttcag ctgaaaatgc cgaagaactg
      421 ttggttcaac gcaaaactga cctggcaatt acgctgcaac cagtaattag tcgctctgtc
      481 atctgcatgc cgttacacac tattcgcaat tcccttatat gtagcaacaa acatcctcgt
      541 attactgata ccagtaacta tgaacaaatt atcgccgaag aattcaccct gttgatatct
      601 aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat aaaccgtaaa
      661 gtttcatttc gcagttcttc tttgttgact ataattaata gcatttctgt tactgactta
      721 ttaggcattg tgcccaccga actgtacgat ttacatcgtg actttttgaa gttaaaagaa
      781 ataaaactgg aacaacctct tcctgcggtt aaactttata tttcctataa caaggcttcg
      841 ttgaacaacc ttgtcttctc tcgattcata gatcgtttaa atgatagctt ctgattcatt
      901 accactgaca aataatatat tacgcatcaa ttaattcatt gtgtgaatat aaaataatgt
      961 gctctatctt gcttcgttta tcctataatt agcggattca tctcacgagt caccccgtaa
     1021 cgttcagaga gcgtttttag atgtctattt ataaaatccc gcttccactg aatattctgg
     1081 aagctgcaaa agaacgtatt acctggacac ttaataccct accccgtatc tgtgtttctt
     1141 tttcgggtgg taaagattcc ggattaatgc tgcatctgac agctgaaatt gcccgacaaa
     1201 tgggcaaaaa aatctgcgtt ttgtttatcg actgggaagc gcagttctca tgcactatta
     1261 actatgttca gtcattacgt gagttttacg ccgatgtcat cgaagagttt tactgggttg
     1321 cgctcccgct tacgacgcaa aattcccttt ctcaatacca acccgaatgg cagtgctggg
     1381 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat cctgactttt
     1441 tctcctttta ccagcctggc atgaccttcg aacaatttgt gcgtgagttt gctgaatggt
     1501 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcagatgag tcctacaacc
     1561 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgatgataag ccctggacca
     1621 ccgccgcacc aggcggtcat agctggtaca tttaccccat ctacgactgg aaagtggctg
     1681 atatctggac ctggtatgct aaccatcaac agctctgtaa cccactgtat aatataatgt
     1741 atcaggcagg cgtccctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
     1801 ggcaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct atgtgcgcac
     1861 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgataatcat ttttatggtc
     1921 atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta ttgctgctca
     1981 atagcatgcc ggaaaaaaca gcggagcatt accgcaataa aatcgccatt tatttgcact
     2041 ggtatcagaa aaaaagcatc acggttccac aaactcagca aggggacatt ggggcgaaag
     2101 atatcccctc ctggcggcga atatgcaaag ttttactcaa taacgattac tggtgtcggg
     2161 cattatcatt tagccctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
     2221 gaaaacgtaa ggaatggggg atactatgca acaacgatta acgcaggatt taacccaatt
     2281 tctcgccagc ttgccggaag atgatcgcat caacgcgatt aatgaaatcc gcatggcgat
     2341 ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg ttaaaaacag
     2401 ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct
     2461 gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa
     2521 aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc
     2581 gttaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca
     2641 acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgtcaccaaa tcactgccat
     2701 gtcagaaatc gtccgagaac tcagccagtt gggatgggac gataataaaa ttggcaaaga
     2761 gctgggaatg gacagcgacg aagtgttacg cctgaagcaa attaacggcc tgcaagagtt
     2821 gtttgccgac cgtcaatttt cccgcgcctg gacggttaaa taactacagc tggcgcaggc
     2881 gttctgctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat
     2941 gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc cgctacgccg tgctcccgcg
     3001 tcagccactg gcaaaactca acatcatcca tcgtagatac ggcgctgtaa tccaccagca
     3061 aaaagtatgt accttcgcac ggtaaaatct ccagccgact ttcatttaag gcattcacca
     3121 gaatatcgcg cttctggcga taaaagtccg gtaatgcaag ataatgctca ggttctgcac
     3181 gtagcatatc ggcaagcgcc agttgtgccg gggtattcac cgaaaaggtc agatactgat
     3241 gcactttgcg gatctcggcg ctgatgggcg caggcgcaac gcaataaccc actttccagc
     3301 cggtcatatg ataggtctta ccaaacgatg acaccgccac cgctcgctca cgcaattgcg
     3361 gatgcgccag cacgctggca tggccctgtt gtgaaaaatt gatgtgctcg tagacttcat
     3421 cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca cagagcggcg aaatcagtct
     3481 gctgccagac agttgcactg gggttatgcg gggtgttgag gatcaccagt cgggtgcgct
     3541 cgcttaacaa tgcggcaaat tcctgccagt caacgcgaaa atgcggcggt tgcagtgcaa
     3601 tacgtttcac tattcccccc gaaagtgcga tggcgggggc gtaactgtca tagctgggat
     3661 caaaacaaat cacttcgtcg ccattgcgca ccaatgcggt tatcgccgcg tataacgcct
     3721 ccgtcgcccc tgccgtcacg gtgatattgc tgtcgacatc cggttgatag ccatacagac
     3781 gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg ggtgcgtatt
     3841 ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc ggaccatcaa
     3901 aatcaggaaa gccttgcgaa aggttaatcg cctggtgttg ctgcgccagc gcgctcatct
     3961 tggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg
     4021 tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg atgctagtat
     4081 ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca acatgcctca
     4141 caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatcccg gaagtttcaa
     4201 tcacctgcac gattttttca ctgatgaaca actttctcgc gcggtgtgga tctacggcga
     4261 acgcgccatt gctgcggcac aaaccaaact gccgcctgcg tttgaactgc caggggtaaa
     4321 gcatattttg tttcgtggtc attgcagcga aagcgacgtg caacaactgg cggcggagtc
     4381 cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactactcg acaccgcgaa
     4441 agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt ccgacgatcg ccgctacctg
     4501 cgccgcctgg acaccgctct ccgtctggta taacgatgcc ggacaagcgc tgcattatga
     4561 gattttcgac gacgccaatt ttatggtgct ggtggaaccg gagattatcc tcaatgcgcc
     4621 acaagaatat ctactggcgg ggatcggtga cacgctggcg aaatggtatg aagcggtggt
     4681 gctggctccg caaccagaaa cgttgccgct aaccgtgcgg ctgggaatca ataatgcgca
     4741 ggccattcgc gacgtcttgt taaacagtag cgaacaggcg cttgccgatc agcaaaatca
     4801 gcaattaacg caatcatttt gcgatgtggt ggatgcgatt attgctggtg gtgggatggt
     4861 cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggcc catgccgtgc ataacggtct
     4921 gaccgttttg ccgcaaaccg agaagtttct ccacggcacc aaagtcgcct acggaattct
     4981 ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg caattaaccg gggcgtatca
     5041 gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata atcaggtgga
     5101 gatcgacaaa atgattgccc acaccctacg tccggtggaa tccattcatt acctgccagt
     5161 cacgctgaca ccagatacct tgcgtgcagc gttcgaaaaa gtggaatcgt ttaaagcctg
     5221 acgtacaaat ttagcagcag cgcgcgccgc cttttccacc atagcgcgcg tcctgccgct
     5281 cacggaaaaa ctcctcgtag gtcatcggcg tttgatcggg atggttaacc cgcatatgtt
     5341 cgacatagtt gtcgtaatca ggcataccaa tcatcagttt cgccgcctga cctaaatatt
     5401 ttccggcttt tgccagtgaa tcaaacatct aaacctccgg taatacaaat gccctcctga
     5461 attaagaggg cttaattgct atcaacacgc tcgggctgag agtaagggga acgttcagca
     5521 catcccccct cactctgact ttagtgcgcg cctttcgcct gcgccacgat ctcctcgaca
     5581 ttttccggca tcggctcata tggcgtttct ttcgccgttg gcttcggctc tttcaatgcc
     5641 gccagtgccg tcttaatcga gaacagcgcc agaaccacca cgaccaccat aaagaagatg
     5701 gttaacccgg catcaagacg gttgttgaac accagttgcg ccagttgtga ctcagtatac
     5761 tgcgatggaa tattgccgct gtcgatcatt gcctggaact tgttagcaat cgccaggaat
     5821 ccgactttcg catccgggct aaacgctttc tgccagcctg cggtcagggt acaaatcagc
     5881 agccaggccg ttggtaccag cgccacccag gcgtaacgtt gacgtttcat tttgaacagc
     5941 accacagcac aaagcatcag cgccatccct gccagcatct ggttggcaat accaaacagc
     6001 ggccacaagg tgttaatacc gcccagcgga tcgacctcgc cctgatgcag gaagtagccc
     6061 cacgccagca cgcacagcgc cgttgccagc aggttagcag gcagtgaatc ggtccgtttc
     6121 aggcccggag acaccacgcc cagcagatcc tgcaacataa agcgcgcagc acgcgtaccc
     6181 gcatccaccg ccgtcagaat aaacagtgct tcaaacagaa tggcgaagtg ataccagaac
     6241 gccacatcca tcatgccgcc cagcgcaccg tgcagaatgt aggccatccc caccgccagg
     6301 gtcggcgcac cgcccgcacg ggaaatgatc gactgttcac ccacttcgct ggcaatctgg
     6361 tttagcgtat ccggggtaat cgcaaagccc cagctactca ccacctgcgc ggcagaagca
     6421 accacatccg ccgtccctgc cggagccagc accgccatcg ggctgttcat ggcgaagtac
     6481 acacccggat cgatgataca ggcggaaacc agtgccataa tcgccacaaa ggattccatt
     6541 aacatcccgc cgtagccgat aaagcacgcc tgcccttcgt tcgccagcat cttcggcgtg
     6601 gtgccggaag agatcagcgc atggaagcca gacaccgcgc cacaggcgat ggtgataaac
     6661 aggaacggga acaggttacc ggtccatacc gggccagtgc catcgacaaa tttagttagc
     6721 gcaggcatgg tcagcgtcgg gcgcataatt aaaatgccca ccgccagacc gacgatagtc
     6781 ccgattttca ggaaggttga gaggtagtca cgcggagcca gcagcaacca caccggcagc
     6841 accgccgcca caaaaccgta gcccaccagc atccaggtca gctgcacgcc ggtaaagtca
     6901 aagtacggtg cccaggtcgg gctttctgcc acccagccgc cagagataat ggcgaaaatc
     6961 aggaacacca gaccaattac cgacacttcg ccaatacgcc ccggacgcaa atagcgcagg
     7021 tagatcccca taaacagcgc cagcggaatg gtgaacgcaa cggtatacgt tccccacggg
     7081 ctatgagtca gggctttcac cacaatcatc gccagcacgg caaggataat gaccatgatc
     7141 ataaagcagg ccaccagcgc tatcaccccg gcggttggtc ccatctcttc tttgaccagc
     7201 tcacccagcg agcgaccgtc acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc
     7261 gcaccggcga gaaccacccc ggcgagcagc cagatcatcc ctggcaggta acccatttgc
     7321 gccgccagta ccggcccaac caacggacct gctccggcaa tggccgcaaa atggtgaccg
     7381 aacagcactt ttttgtccgt cggcacatag tccagcccgt cgttatggcg caccgctggc
     7441 gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacaggcc ataaaaacgg
     7501 taggcgatta gatagataca gaccgacgcc accacaatcc acagcgcgtt gatctgttcc
     7561 ccacgattta aagcaatgta tcccagagca aatgctccca ttacagcgag cactgtccag
     7621 acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt
     7681 acattttgtt tctataacat ctttgtacaa tttaacaaca cagcaaccat gtaaatcgga
     7741 attgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac cgagttttcc
     7801 tgcaatctca tttaattctg tcgggctcat cccaaaactg ccgtacccag ccgacaggtg
     7861 cagcagcgcc gcccctgttc atcgaaaacg acaatttccc agctctgatt ttgtcgtcca
     7921 agatgcagcg gctggcagac accgcgcacc tttccctcag acaccgggcg atgatgtgtg
     7981 gcgttaagtt ccgtgcccac cacacactgt ccgtcgcggg tcatcataaa tccggccatc
     8041 gatcccagcg tttccgccag cgccgccgac gcgccgccat gcagcaggcc aaacggctga
     8101 tgagtacggg tatcaaccgg catttcggct tcgagtacat catcgcccag acgggtatac
     8161 acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagctc gtcgagcgtt
     8221 aaatgacgtt tccagatcat gcttatgccc ccagcgttga gccgccatcg accacaatat
     8281 cctgtagggt aatatggctg gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct
     8341 cttgtggaca ggcgattttc cctagcggaa tgccgagttt aaactgctcg ccaaagccgc
     8401 gaatacgctg ctcttcggcg tcatcgctca cccacagcgt gcgttgcata tcggtgtcgg
     8461 tggagcccgg cgaaaccaca ttacagcgca cgccgctacc cgccagttcc agcccgacgc
     8521 tcaacgccag gcttttcagc gccgctttcg acgcgccata agcactcata ccaatacgcg
     8581 gcgtgtgtgc ggcgtcggac gcaacagtaa caatcgcccc gccccgctga cggcgaaact
     8641 ggttcatggt ctgctgaaac aggttaaacg caccgccgac gttgaccgca aaagtctgct
     8701 gccagtcctc tttactgagc tgatcggtcg cgcccatgcg caaaattccc gccgcattga
     8761 ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc gccacctgcg
     8821 cagcatcggc aacatccatt acttcggtcg caaaaggata ttgctcctga ctaaacgctt
     8881 gatcaaaacc tgtaactttc gcccccgcct caacaaatgc cagcgccgtg gcgtagccta
     8941 tacctttccc cgcgccagtt acccagacat ttttaccgct gaaatccatt atttcacctc
     9001 gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccagca tgacaaagtc
     9061 gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag
     9121 accgtagtcg atcaggttgt cgtcatcgaa gggttcatcg gactcgtcca gcaacggcag
     9181 gatcacctca cgcagcgccg ctttgctggc ggggataggt gctggcagca attcttcggt
     9241 catcaccacc cgaccagaac gtccggccac atatttcagc gacatcaaat gctcgtcacg
     9301 gctgaaatcg gccagcgcat ccgccaccat aaacggttta atatcgcgca taaatgcgtc
     9361 cgttgcggtg gtcatacagc caatgtgggc atataccccg gtaataatca gctggttacg
     9421 tccactctct ttgagcattt gctccagcgg agaacgatga aatgcgctgt agcgccattt
     9481 caccagcacc gtgtcgtcgg catctggcgt caggcgatcc accacctttt gctgttccgg
     9541 cgaacgggtc aggccaggcc cccacatatc attcaacagc gcccgatctt catcgctctg
     9601 ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt agtcgcgtag
     9661 tgccgcaata ttcgcgatca cctgctccat catcgggcag ttctcacccc agaagctgac
     9721 aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa aggcccagtc
     9781 gactttattc tgcggaatat cgtgaggctc cggcagtgcg taagcctgta attttggaat
     9841 agccatcgtg ttctctcctt caggctgatg tgcgtgacgc cagccactga cgtaattgtt
     9901 ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc tacacactcc acgcgatccg
     9961 gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc accgcgcgca
    10021 gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca tcttccatgc
    10081 tcaccagtgc ggcgtagatc accgccggat ggcgcagcag caggttttcg atctcttcgg
    10141 cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc acagtgatgt
    10201 aaccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg gcatcaaagg
    10261 cgctggcatt gtgctgcggg cttttgtaat agccacggaa agtgtacggc ccgcgcgtca
    10321 tcaggcgtcc aacttcccct tgcggcagtg gatttccttc ggcatcggca acccatactt
    10381 catcgtccgg gcacattggg taaccctggg tatggataat tttctccgca ctatcatcaa
    10441 gacgggtgta gttcaccagc ccttccgcca tgccaaacac ctgctgcaac tggcagccaa
    10501 tctcagcggg aatacgcgcc gcaagcgtgg cagaaagacg tgcgccgccg acctgtaaca
    10561 gtttcagcga ggcaagctgc gcccggcttt cgccttcagc cagtgcctgc aaccacaggc
    10621 tgactgctgg cggcaccagc gcggtgacgt tcacctgatg tttttcaatc aatgggaagc
    10681 aaagcgtggc gctggggtcg gcagccagaa cgacagtgcc gccagcgaga aataccccca
    10741 acgatcccgg tgaactcatg gcgtagttat gagccgccgg gatcgcgcac aggtagcgcg
    10801 tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag tagtcgttat
    10861 gagtgcgcgg gatcagtttc ggagtgccgg tggtgccgcc ggaaagctgg aaataggcca
    10921 cttcatcagc aggagatggc gtggcagtaa aatcctcagc cggatggtta atcgcatcct
    10981 gcaagttatg ctcaccgctg tcgttgagca gctgcaccac gcgaatggaa gaatgctctg
    11041 caacaaatgt gttgaggaaa tcatccccgc taaatagcgc atgttggcga tcggcaatca
    11101 gtaacgccgg ttcaatctgg ctggcatagg cgttcagttc actacgctga tggctgaaca
    11161 gcgccagcac cggcgcaacg cccagtttca gcagcgcgaa aaaggtaatg taaagttcag
    11221 cgacgttacc cagttgtacc agcgcggttt caccaggttt aatgccctga cggcgcaaac
    11281 tacaggcgag gttatccgcc gcctgattca gctcccggta actcaactgc cactcgccgt
    11341 cgatcaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc agtggcaaat
    11401 cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccat cgggtgaatg
    11461 gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta gaaagtttga
    11521 cgcctgtttc gcgccactca cccaacggtg aggacgcagg cacaatcccc gctccggcaa
    11581 acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc cattcaccgt
    11641 taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg aacggctcca
    11701 gttcagcaat aacctgagtc gcggccagat gcgggaaacc gctcagcgca ggagttggat
    11761 gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct tcaaagggag
    11821 ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact
    11881 cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca tgttcatggc
    11941 gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact tcatctggct
    12001 gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct ttacgtagca
    12061 gcagttccgg gctggccccc agcagaacgc caccatcagc caacggaaca tggaagttgt
    12121 aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca atggcggcgt
    12181 cagtggtgat atcaatcaac cgtgacaaca ccactttgtc gacctgcggc gtggcggtaa
    12241 gtgcggcagc acgggcaacc atctgttcaa acgtggtttg ttccggaatg gcctgacgtt
    12301 ccaccacgtt cagcgactgg ctgcgggtga aacggcgtgc ggaagcttgt ttttcctgac
    12361 gggagaacga ctgccaggat tcgggaatat acagcgacga aggctgacgt ggatcgaagg
    12421 gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg gcaaacagcg
    12481 cggcgagttt ttgctggaag ggactgtcgg gtgaatcccc gttcacagcg ggttcatcga
    12541 agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat
    12601 tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta tccatatcat
    12661 cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg gtcgctaacc
    12721 taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac tcggggttgc
    12781 atctggttgt ggttacaatg aaaatgagaa gcattaactt cattaataac aggaagctga
    12841 tttgtgagac tcgcctcgct ctaccgcaac gcccttctat taacaggact tttgctttca
    12901 ggaatagccg cagttcaggc cgccgactgg ccgcgtcaga ttactgacag ccgtggcact
    12961 cataccctgg aaagccagcc gcagcgtatt gtatccacca gcgtcaccct gactggctca
    13021 ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacaccaaa taaccgcgta
    13081 gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa
    13141 cggctctata tcggcgaacc gagtgcagaa gccgttgccg cgcaaatgcc ggatctgatt
    13201 ttaattagcg caaccggcgg cgattcggcg ctggcgctgt acgatcagct ttctaccatc
    13261 gccccaacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt
    13321 ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcactgtt tgataagcaa
    13381 ctggcagcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc ccttgtctat
    13441 accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg gcagatgctg
    13501 gaacaactcg gctttacgct ggcgaagttg cccgcaggct taaacgccag ccaaagccag
    13561 ggcaaacgcc atgacatcat tcagcttggt ggagaaaatc tggctgcagg gttaaatggc
    13621 gagtcactat tcctgttcgc cggtgatcag aaagacgccg atgctattta tgctaatccg
    13681 ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt atgcgctggg aaccgagaca
    13741 ttccgcctgg attactacag cgccatgcaa gtgctggaac gacttaaggc gctgttttaa
    13801 gcattaattg tcggacgctg tcacctgcgg cggcgtctgg cgaaaacgcc gcaactccac
    13861 cagcaccagc aacaacaaca cgccgataat caacaaacca aaaccgctcg cgcttgcgga
    13921 agcaaccggt gtcatcatcg ctcccagacc gcctaacagc gccgcaccta tggcatcgcc
    13981 cgtcacgttt tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt ccggggtttg
    14041 cgtttgcagc attgtatatt gcagcaacga actaaccgcg ctcagccagc cgaacagtgc
    14101 cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa tggcgaggaa
    14161 cgcccccaat gtggagagca acatcaacag acctggccgt acgctatgcg ccagttttcc
    14221 actggttaac gcgccaatag ccgcaccgag cgggattgcc gcatagagaa agccaatttg
    14281 cgccgccgac atctgccagt tgtcagccag cgccggatac agtacccgca ccgcgctcgc
    14341 catcgtcaat aaacccccca gcaacgcaat ccctcccacc agcgggctgg cgagtagaaa
    14401 acgaaatcct gccagtaatg atttcaacgg atgctcacgc ggctgcggcg gtggtggcaa
    14461 cgccggaagg cttaacaacg gtagcaaggt aataaacgtg cccgccgccg ccagcccgta
    14521 gttccaggct acgcctccgg tcgccagcag taaaccacca atcatgggcg aaatcaccga
    14581 ccccagacgc acagtcaaca tggtgatcgc cccggcctgc attaggtttt cacgtcctac
    14641 cagtgcaggt gtcgccgcca gtagcgccgt aacgccaagt gatgcgaaaa aaccatccca
    14701 taaaccaagt aaatagattg ccagcaaaga cggctccggc agcagcgcat tcaggcacag
    14761 tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgctcata
    14821 gcgatccgcc agcacaccac cgatcatcag accgacaaac atagcaccgc cggtcagcgt
    14881 caccgaaagc cccacctgcc aggtagaatg tgtcatcatc tggatctgca ccggcaccgc
    14941 gacgctgagc aaacccagag acacaattga gataaaacga gcgaggaata ctgcgcgaaa
    15001 cgccgggtgc gtttttaaca ggctgaggtt aagcagccag gattgtttat tcattacaat
    15061 gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa catatccaaa
    15121 taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct ggttctgttg
    15181 ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat
    15241 taagcctgct cattggagca aaatcactcc ccgcttccgt agtgctggaa gccctctccg
    15301 gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc acgactgccg cgcacccttg
    15361 ccggtttact ggcaggcggt gcgcttggcc ttgccggggc gttaatgcaa accctcaccc
    15421 gaaacccact tgccgacccc ggcttgcttg gcgtgaatgc cggagccagc tttgccattg
    15481 tgctgggtgc ggcgctgttt ggttactctt ccgcgcagga acaactgatg atggccttcg
    15541 ccggcgcgct ggtggcgtca ttgattgttg cctttaccgg cagccagggc ggcgggcagt
    15601 taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg gaaggactga
    15661 ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc tggcaagccg
    15721 gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatcccg gtgcttaccg
    15781 ccggagcaac cgcgctatta ctgagccgcg cgctgaacag tttaagcctt ggcagtgaca
    15841 ccgcgacggc gctgggcagt cgcgtagcgc gcacacagtt gattggtctg ctggcaatta
    15901 ccgtgctttg tggtagtgca acggcggtag ttggcccgat tgcctttatt ggcctgatga
    15961 tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg ctggtcgctg cccgtcacgc
    16021 tactcgctac ccctgccctg ctgctgtttg ccgatatcat tgggcgggtg attgttcccg
    16081 gcgaactgcg cgtttctgta gtcagtgcct ttattggtgc accagtgctg atcttcctcg
    16141 tacgacgtaa aacgcgaggt ggcgcatgat ttacgtctct cgccgattaa ttatcacctg
    16201 tctactgttg ctaatagctt gtgtaatggc aggcgtgtgg ggattacgca gcggtgccgt
    16261 cacgctggaa acctcgcagg tattcgccgc gctgatgggc gatgcgccgc gcagtatgac
    16321 gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcggc
    16381 gctgggcgtc agcggcgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga
    16441 cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg
    16501 tcaggatctg acggctatcg cgctggcagc aatggcgggg ggtattgtca cctcgctgct
    16561 ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta ttatcggtat
    16621 cggcgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga
    16681 aacggcgcta acagcgggtt tgtggaatgc cggatcgctc aatggcctga cgtgggcaaa
    16741 aacctcacct tccgcaccca tcattatatt aatgttaatt gccgccgcct tgctggtgcg
    16801 gcgaatgcgc ttgctggaaa tgggcgatga taccgcgtgt gcgctgggcg tcagcgtcga
    16861 acgttcgcgg ctgttaatga tgctggtcgc cgtagtgctt accgcagctg ccactgccct
    16921 ggcggggcca atttccttta ttgctttagt cgcgccacac attgcccgcc gcatcagtgg
    16981 caccgctcgc tgggggctga cccaggcggc gctgtgcggg gcacttttac tgctggcggc
    17041 cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt
    17101 cagcctcggc ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc
    17161 gaatcagtag cccgtttgcg tggcgaacag ttaaccctcg gatatggcaa atataccgtt
    17221 gcggaaaatc tgactgtaga aatacctgat ggtcacttca cggcaattat cgggccaaat
    17281 ggctgcggta aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc tgctcacggg
    17341 catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaggt tgcacgccgg
    17401 attggtctgt tggcgcaaaa tgctaccaca ccgggcgata tcaccgtgca ggagctggtg
    17461 gcgcgtgggc gttacccgca tcaaccgctg tttacccgct ggcgcaaaga agatgaagac
    17521 gcggtaacaa aagcgatgca ggccacggga ataactcatc tggcggatca aagcgtggac
    17581 accctttctg gcggacaacg ccagcgagcg tggatcgcga tggtgctggc ccaggaaacg
    17641 gcaattatgc tgctcgacga acccacgacc tggctggata tcagtcatca gattgatcta
    17701 ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc ggtgctgcac
    17761 gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt
    17821 gttgctcagg gagcgccgaa agagattgtc actgctgaac tgatcgagcg catttatggt
    17881 ctgcgctgca tgataattga cgatccggtt gccggaacgc cgctcgtggt gccgctcgga
    17941 cgaacggcac cttcaaccgc aaagatttaa actaagtggt ctgccatcat ggcatcctgt
    18001 tttctggatg ccatcgcatg acgcaacaac acaccaccac aagccaccat cccgccgacc
    18061 aacgcagaaa ggatcacgat aatcgcctta ccctgaccgt ctttttttac gggtaatgac
    18121 ggacgcaact gatatttaaa cggtgtaaaa ttcacatcgt tgacatttgt ttttgttaat
    18181 tgctcgacta gatactgacg attacgtaat tcaccgttca attccgcaac gtcagtgact
    18241 gctttttcaa tttccagttt gcgttcaata ccgtctgcac cgagagaaat agagaaatcg
    18301 gggtcatctt taacggcctg accattactg tatacgggct ttttaattcc tgccgcgttg
    18361 gcaatgtcaa gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcgtttta
    18421 atgcgatcct gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg
    18481 acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata cccggccaac
    18541 actttctgtg cctcttcact ggtgggtgcg gtgaaactta gcgtccagga agtatacaat
    18601 gcagactcat cttttttctt actggcattg tcatcaaccg ctttcatttt ttcgctcaat
    18661 gcaacaatgg cgcgatgcag atccagtggg tcgattttcg cttcttttaa ttgatccatc
    18721 acataaggtg atgaacgcag gtactcctca agcaagctaa cagactgaaa tttcttgata
    18781 aacaggttaa atgcttctgt acgatcaatt ttgatatcca gatccagcac acgaagctta
    18841 gtgaatgttt tctccagctc ttgccactga acaggttctg gaggcgtgac aaccgccgcg
    18901 ctagtccatt tttgcggcag gatgaaagag atcagcaagc ctgcgcaggc aaacgcaaaa
    18961 acgaccgcca tgaccgtttt ttttgcccgc cataaaacct cgattagatt aagtaaatca
    19021 atttcattat tactgggcga cgccagagga tactcgggaa aatgagcttc acttccctgt
    19081 ttaatattca gtgatgacat gtagtgaaac ctgaggtttt agtccgtaaa gcacaacgat
    19141 gtacatagca ttctaacagg aactgagatt t
//
LOCUS       DAPQZD010000083        19127 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPQZD010000083 DAPQZD010000000
VERSION     DAPQZD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.083"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(51..650)
                     /locus_tag="RO454_002868"
     CDS             complement(51..650)
                     /locus_tag="RO454_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDY0934672.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDDRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(718..4113)
                     /locus_tag="RO454_002869"
     CDS             complement(718..4113)
                     /locus_tag="RO454_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDY0934673.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDNVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVSSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVQVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     AAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIIDIRQVETSARYLGTALYWIAASSNIKPGYDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLNFYKGLINKTHLGKELLENFELTEDNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPA
                     GHGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKATAGITIS"
     gene            complement(4174..4821)
                     /locus_tag="RO454_002870"
     CDS             complement(4174..4821)
                     /locus_tag="RO454_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDY0934674.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGHRIDLRVKTGAEAIRAL
                     ATQLPAFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTATSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4719..5462)
                     /locus_tag="RO454_002871"
     CDS             complement(4719..5462)
                     /locus_tag="RO454_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0934675.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMSPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLATASTFV"
     gene            complement(5468..6166)
                     /locus_tag="RO454_002872"
     CDS             complement(5468..6166)
                     /locus_tag="RO454_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDY0934676.1"
                     /translation="MQDIQQETLNECTKTEQSALIVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGYLSINKLSQ"
     gene            complement(6166..6522)
                     /locus_tag="RO454_002873"
     CDS             complement(6166..6522)
                     /locus_tag="RO454_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0934677.1"
                     /translation="MEDCTVPEPEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGG
                     LNADLKTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISD
                     NAMYTDFNCTFEEVTH"
     gene            complement(6500..9727)
                     /locus_tag="RO454_002874"
     CDS             complement(6500..9727)
                     /locus_tag="RO454_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDY0934678.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISFADEWNSVNARLKQAS
                     QSSDDFQASQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNENGDRVIRALAAGVGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLAAAQASLTRNIAART
                     AAQTTLNTVTSVGSRLLSGALGLVGGVPGLVMLGAAAWYTMYQNQEQARESARQYAAK
                     IDEIRQKTSAMSLPEASDNEEKTRQALDEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQTALNNSKRELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLDAW
                     RNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASTRASNDAQLLGYGEGSRMRER
                     LQEMWSIRHEFEQKNNELLRQYQAGEIEEALWKQEKELNKKYLEERLSDQQDYYAKAD
                     ALRNNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSILQEVSKILMNAAIVNGLKSLSGAGGWLGTVGGWISGAVANAKGGVYTSANLSAYS
                     NTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNSGGGVVYSPVY
                     HISIQNQGSNGEIDARSARGLVDLIDSRVVSIMQSSRRDGGLYSA"
     gene            complement(9774..10052)
                     /locus_tag="RO454_002875"
     CDS             complement(9774..10052)
                     /locus_tag="RO454_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDY0934679.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLENALLIVSQ"
     gene            complement(10076..10447)
                     /locus_tag="RO454_002876"
     CDS             complement(10076..10447)
                     /locus_tag="RO454_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HDY0934680.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(10462..11166)
                     /locus_tag="RO454_002877"
     CDS             complement(10462..11166)
                     /locus_tag="RO454_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HDY0934681.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTEPLKVKADLKSTLRVA
                     SGAKLEMAVEAAGGVPPYSYVWKKGGSPVSGQTAATFSKASAVSGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(11227..11571)
                     /locus_tag="RO454_002878"
     CDS             complement(11227..11571)
                     /locus_tag="RO454_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDY0934682.1"
                     /translation="MREATLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADNIREQALDAVRKLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(11568..12014)
                     /locus_tag="RO454_002879"
     CDS             complement(11568..12014)
                     /locus_tag="RO454_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDY0934683.1"
                     /translation="MDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDAV
                     VERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAFY
                     WRFVELGTINMPAHPFIRPAFDTTEELAARIAIQRMNQAIDEVLSK"
     gene            complement(12014..12352)
                     /locus_tag="RO454_002880"
     CDS             complement(12014..12352)
                     /locus_tag="RO454_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDY0934684.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQATVRVWMRFRRDVTATSRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            complement(12361..12678)
                     /locus_tag="RO454_002881"
     CDS             complement(12361..12678)
                     /locus_tag="RO454_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDY0934685.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(12755..13972)
                     /locus_tag="RO454_002882"
     CDS             complement(12755..13972)
                     /locus_tag="RO454_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDY0934686.1"
                     /translation="MKLHEMKQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQSVTTDSQGGYTVPTQMRNKIIDTMKAYGGIASVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTVASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEEMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            complement(13987..14586)
                     /locus_tag="RO454_002883"
     CDS             complement(13987..14586)
                     /locus_tag="RO454_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDY0934687.1"
                     /translation="MNDRETRCYSGEVRAEQYDNAPTHILGYGSVFNSRSEPLWGFRE
                     IIKPGAFDDVLNDDVRGLFNHDPNFILGRSSAGTLSLSVDERGLRYDIVAPDTPTICD
                     LVLSPMLRGDINQSSFAFRVARDGESWYEDDEGIVIREITRISRLYDVSPVTYPAYQD
                     ADSGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            complement(14579..15805)
                     /locus_tag="RO454_002884"
     CDS             complement(14579..15805)
                     /locus_tag="RO454_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0934688.1"
                     /translation="MLLDALFRSEPLENPSVPVTGEAAETDNIFARDVYVSPETSMRL
                     AAVYACIYVISSSVAQMPLHVMRKTNEHVQPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWSRLKNMWQRAVTALRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKNDE"
     gene            complement(15795..15956)
                     /locus_tag="RO454_002885"
     CDS             complement(15795..15956)
                     /locus_tag="RO454_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934689.1"
                     /translation="MKQLITDMTGLIGFGLLTAGVYLYAGLPASLMLSGCLLLLYALV
                     VSMRRKHAS"
     gene            complement(15953..17710)
                     /locus_tag="RO454_002886"
     CDS             complement(15953..17710)
                     /locus_tag="RO454_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDY0934690.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEKRGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADSEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPQHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVALFTAMSRFLVNG
                     GGVNDFLSTLDPDEDLLIL"
     gene            complement(17710..18192)
                     /locus_tag="RO454_002887"
     CDS             complement(17710..18192)
                     /locus_tag="RO454_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDY0934691.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDLKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRSKTQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDNVDPLAELLKARD
                     "
     gene            complement(18340..18690)
                     /locus_tag="RO454_002888"
     CDS             complement(18340..18690)
                     /locus_tag="RO454_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDY0934692.1"
                     /translation="MPPRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
BASE COUNT         4323 a         5735 c         4403 g         4666 t
ORIGIN      
        1 aaagcccgcc tgaaccggcg ggctgtcata acactgtgtt acctggctaa tcagaactta
       61 taaccgacac ccacgatgaa accgtcagtg cgccagtcgc cactgccgga gccttcataa
      121 gcaatatcaa tggccacgga ttcggtcggg ttaaactgca cgccagctcc ccacgccaga
      181 gacgtgttgc tgtggcgatc gtcatcactt ccggtcagca catcgtgcgt tttccccttg
      241 ttgtcagtta cgcggagata atccccggag aacgtcgaaa cacggctgta agccacacct
      301 gccatcgcat aagcactgaa ccattcattc acgcgtacag atggccccgc catcatgctg
      361 aaccagcggt tacgcactga atcttcatgc cagcgggtat cgctgtaatg cgttttttgc
      421 tcatctttgg cattggcata actgaatgac gtcaccagcc ccagcgtgtc cgtaaattca
      481 taacggtatt tcacgttaat gcccttcagg tcatcactgc ctggcatatc agtatgggtc
      541 tgaagatacc cggcgcttag tgtggactga tgctctgctg cgctcgctgg cgtaccagcg
      601 gcaaccagcc agactactgc ggatagaata acagcacata atttacgcat aattacctct
      661 cgcttttctg caataaaaaa ggcaccattt ctggtgcccg tatctgggtt ataaaattca
      721 gctaatcgtg atgcctgcag tggctttctt catcacaaca accagcaaat cgctgatact
      781 tgctgtggga taccagccat ttaccagcca tgctgacacc gaaaactcca gcgtcatgtg
      841 accgtgaccg gcaggcatat caataacgcc actgtaaatc agcgtattat ccagcgcggt
      901 acggttataa atttcagcac cgtttttccg cactatcaga cggcatgagg agtaaatatc
      961 agtatgctct ctctcatgct tagcgccact gaatgccaca gccggaataa caatttgccg
     1021 gtcaaacggc tgatcgtcat aaaccctgac ggtaatggtc cctgatggcc accgctccgg
     1081 tgcccgggag tcacggggga aagctttgcc cactgtttta acgagatcgc cttcaatctg
     1141 gttcgcggac agttttccca gaacccggca gttctcgtta atcgtgacgt tgttgagcgt
     1201 cccggagttc gcattcacgt taccgctgat atcggcattt ttcgccgtca gccgcccgtc
     1261 cggtgtcagg gaaaatgccg gaggattacc gccgctggta atggtggggg ccgtcaggcg
     1321 cttcaggaac acgtcgttca tgaatatctg gttgccctgc gccacaaaca tcggcgtttc
     1381 attcccgttt gccgggtcaa taaacgcgat acggttagcg gcaaccagga actggctcag
     1441 tttgccttcc tccgtatcct ccatgctgag gccaagcccc gcgacataat gtttgccgtc
     1501 tttggtctgc tcaattttga cgccccacat ggcattccat ttatcgttgg cgtccttcca
     1561 ctctttcgaa aactcctcca gtttgctggc gttatcttcc gtcagctcaa agttttccag
     1621 cagttccttg ccgagatgcg ttttattgat caacccttta taaaaattca gataaccttc
     1681 cgcatcatcg ctcgcccgac caacggcctc cacaaatgcc gatttaccaa cggtgttcac
     1741 gctgcggata taaaagtaat aatcataacc cggcttaata ttgctactgg cggctatcca
     1801 gtacagcgcc gtaccaagat aacgcgcgct ggtttcaacc tgcctgatat cgataatccg
     1861 cttttccgag aaccagaact caaactgcac cgtcgggtca tatacagcca gtttcgggac
     1921 cgctgttatc tgaaaatacc ctggtatcag ttcaatagtg acaggcgctg ccggtgccgc
     1981 aatccggaac gataccgatg ccggatcgcc ctgctgcccc cacgcattta ccgcccggac
     2041 cgtcagcgtg taacgcccca gcgccagttg cctgaagcgg tatgtggttt ccgtcgtccg
     2101 ggccgtgctg accagccgct cactgccgtc gtccgctgtt acggtcagac ggagcaggaa
     2161 gctcacgccc ttcaccacct tcggtgtgtc ccatcgcgcc agcacctgat attccccgct
     2221 gtctgcagtg acttctgcgg ccaggtgctg caccgctggc ggcgtgacac cattcaccgt
     2281 gccgctctgg tcgccgtcaa agtgcgcccc gttatccacg atggcttctt tttccggtac
     2341 atgctgcacg gcggtgatgg catacgtgcc gtcgtcgttc tcacggatac tcacacagcg
     2401 gaacaggcgc tggcgcaacg tcggcaactt cagcccccac acgctgtatt ctgcaacgcc
     2461 gtcaggaacc cggctcactt tcaccttcac gccgtcggtg acggactgga cctgcacgct
     2521 gaccggattg ccacttccgt caaccaggct tatcagcgtg gtgccggagg atggcagcgt
     2581 gatttcacgg tcgagcgtca gcgtccgggt ctggctgctc accgccagca cacgaccacc
     2641 ggtgctgata ccggcatagt catcatcgca gatttcaata acatcgcccg gtacatggcg
     2701 aagcccttct gcgcccacgc tgaagtccac ggtctgcgtt tccagcagct ccgttttaat
     2761 cagccacagc ccggcgcggt gtgcctgccc ccggctggta cagccaaagg catccatctt
     2821 cgtgacatta cgaccataac gggcaatggc ctgcgtgtcc tccacaagct ctgttgccgt
     2881 ttcccagccg ttatccgggt cagtccagtt cacctcaacg gcattatggc ggtccttcag
     2941 ggcgctgaaa ctgtagcgga acggcgcgcc atcatccggc atcaccacat tactgcggtt
     3001 ataggtccac acattatccg acggccggtc ctgcacgaac gtcagcgtct gcccgttcca
     3061 taccggcata cagcgcatcg ccgagcagaa atcactgagc acatcccacg ccttacgctg
     3121 tgtggtcagg tacgcattac aggtgatgcg cggctccgtg ccgccaaagc cgtccggcac
     3181 cgactggtcg cagtactggc cgatgacata cagcgcccat ttatccacat ctgccgcacc
     3241 aagacgtttt cccatgccgt agcgcggatg ggtcagcata tcccacagac accaggccat
     3301 gttgttgctg tatgccggct taaacgttcc gtcccagata ccgctgtatt gccgcgtctg
     3361 cgggttatag ttcgacggca cctgcagaat gcgcccgcga agatgataat tacgactcac
     3421 ctgctggctg ccgaactgct ccgagtccac ctgtacgccg accagtgccg tgttcgggta
     3481 gcactgtttc acatcgatga tttcggtgta tgacgaccag agcgttttgt tctgcagctg
     3541 gtctgtggtg ctgtccggcg tcatcctgcg catccggata ttaaacgggc gcggcggcag
     3601 gttatccacc accactgagg ccagatactg tgaggtggtt ttacccttaa tggtgatgtc
     3661 tttttccgtc acccagccac cgttacgttg tatctgaacc agcaggcgga cttccgatgg
     3721 attcctgtcc ccctttgagg tggtttccac cagtgcctgc acaccgaagg taaagcgcag
     3781 acggtcgatg tttgccgacg tgatggtgcg ggtgatcggc gtgtcgtatt tcacttccgt
     3841 acccagcacc gtctcggaac cggaggattc aaatccctcc ggcggtgtct gctcctgctc
     3901 acctgcccgg aacaccaccg taacaccgga gatgttggta ttcccctcac tgtccagcac
     3961 cggtgtactg ttcagcagca cgctttttaa tccatccacc ggaccttcaa tcggcccttc
     4021 gctgatggca tcgatcacac tcagtaattg cgtggatttc aggttgtcct tcgcttcgcg
     4081 cggggtatgc cccttactgc ttcctttgcc cattcgtcat gctccataaa cgacaaaacc
     4141 gcccggaggc ggtttcacat gaaatatttt gcatcagcgg ccaatcacca caacctgacc
     4201 accgtcccct tcatctgccg tgctgatctc ctgagaaacc acacgagacc ccacgcgcat
     4261 ttcaccgtac agaacaggca gaacattgcc ctgggcaacc atgttatcca gtgatgagaa
     4321 ataggtgttc tgcttaccgt tatccgtgct ggtcgctgtg ggcgtccggg ctttcggtgc
     4381 cagcatctgg gccacaccac ccaggatcat actggcccct gccgcataca tgcccgatac
     4441 agccgcggca cccagccagc ccacagggtt ccaccatgcc accgcaatca gcgccgcccc
     4501 cagcaccacc tgaaacacac cgccactttt agctcccgcc agacgcggca cgatgtggat
     4561 cacggcacca tttgccagcg gctcattaag acgggcagat aattcgtttt cgcctgcatc
     4621 acgcccggca atgcgcacct gataccagcc ctcattcagt ttctgacgaa acgccgggag
     4681 ctgcgtggcc agcgcccgga tagcttcggc tcccgttttc acacgaaggt cgatgcggtg
     4741 gccaaatcgt tgcaaatccc cgtaaaggca gatgcgtgcc atgcccggtg acgccagagg
     4801 gagtgtgtgc gtcgctgcca tttgtcggta tacctctctc gtttgctcag ttgttcagga
     4861 atatggtgca gcagctcgcc gtcgccgcag taaatggcgg catgattcgg caccgatgaa
     4921 ccaaagcagc acagcagcac atcgcccggt tgtgctgatg acaacggcac ctgatacagc
     4981 cctgtggcct ccagattatc cagatagaga ttctgaccgt gacgccacca gtcatcctca
     5041 cgatgaaaat ccggcatctc aatccccgcc agatgataag catcccggaa cagcgtgtaa
     5101 cagtccgtca ccccgtgctc aaagcgccgc ccggtaagat gcggcacaca gcggaactta
     5161 tgaatcgccc cccggcagac cagccaccac ggcaaatcac tctgcacctg cagccgcctg
     5221 tcagcctcac tcagccaggg cagaccaccg gggtgactgt ggaccagcgc cacaatctca
     5281 ccctgcattt tggcctgcag ccagtcctcc ggcgacatcc ggaaataatc ttccggctca
     5341 ccggagatat tcacgcaggg gaaatatctt tccccctccg gcgttctcac cacgaagccg
     5401 cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa tcgctgattc tgtctctgtc
     5461 atgggattta ctgcgaaagt ttgttgatgg aaaggtagcc gccaaagttg ccgacgttat
     5521 tgcgaaactt acagccactc aggcatttgc tgcatttatc tttcgtgata tcggacgtcg
     5581 gctggtcata ttcatccgcg acagccggac cgctataacc gcactcatca ccgcgatagg
     5641 tccaggtaca ggtgttggcc agcatgatgc gccccggaaa aaccgcacca tccgtttccg
     5701 tcggcgtgga cagtacaaaa gaagcactga ccgcactcag ttcgctgcac tgctcgatgc
     5761 gccagcggct gatcacctcc tgctccgggt cggcgtcact gtttccgttg acgaagttca
     5821 ccgcatccag aaaacgggcg taaaccttac gcctgaccac cgttccgccg accagactct
     5881 gcaggtcttc cgccatcccg gtgaccatgc cgtgcaggtt agagaccgtc agtgtcggac
     5941 gggcagcact gcccttaccg ttcagttcaa atcccgtccc ctgaatgggg tatgcctgat
     6001 actgccgccc ctgccaggtg accggttcac ctttttcgtt ctgctcatta cagaagaaat
     6061 aacgatctcc gccgacctct gtcagatcaa tttcccagag cacgatcagc gcggattgct
     6121 ccgttttagt gcactcattg agtgtttcct gctgtatatc ctgcatcagt gagtgacctc
     6181 ttcaaaggta cagttaaaat cggtatacat ggcattatcc gaaatgctcc actccctgca
     6241 gacaacccgg acagtcctgt tgtgttttgg cggacgccac aaaaaagcac gaatcccggc
     6301 atgacgggat aaaaaactgt ccagcgcggc acgggaatat tcatctgtga cacgaaatac
     6361 cggtttaaac gttttcagat ccgcattcag accaccagcc cgtctctgtt catatccgtc
     6421 accaaacttt accgtaataa ctgatggctt tcgtgtcgtc tccatcccct cacgggggat
     6481 ccagttaaaa acttcaggct caggcactgt acaatcctcc atcccgacgc gatgactgca
     6541 taattgacac aaccctgctg tcgatcagat ccaccagtcc cctggctgag cgcgcatcta
     6601 tctcgccatt gctcccttga ttctgaatgc tgatgtgata cacgggagaa taaacaaccc
     6661 ccccgccact attcacattg ccaatggctc tgaccccaag agagccgtcc gctgcccgtg
     6721 tcagtggcat gatagcttca ggcccggcct cgcccatcag cccggcacct ttcgcaaaag
     6781 caaaatacgt cggtgtatcc acaatagtgt tactgtaagc actcagattt gccgatgtgt
     6841 aaacaccacc ttttgcgttt gccaccgccc ccgaaatcca tccgccgacc gtaccaagcc
     6901 accctccggc accggagagt gacttcagtc cgttaacaat ggccgcattc atcagaattt
     6961 ttgaaacttc ctggagaatt gaactccccc agtttctcca gtccacaaca tttccggcca
     7021 gcgcatcgga aatatttgat accagcccgt ccatagtgga aacgacagca tctgccgcct
     7081 gcgaagcata atcggtggca ctgtctgccc agttggtcag cccctcctgg agtcctgcat
     7141 tccagttatt acgtaaagca tcggcctttg cataataatc ctgctgatcg ctgagacgct
     7201 cttccagata ttttttattc agttctttct cctgtttcca cagggcttct tcaatttctc
     7261 cggcctgata ctgtctcagc agctcgttat ttttctgctc aaactcatgc cggatactcc
     7321 acatttcctg gagtcgttca cgcatccgtg agccttcacc atatcccagt aactgcgcgt
     7381 cgttagacgc ccgggtactg gcattactgt ccgccaggct gctttcatac gcagcaagct
     7441 gctcacgaat ctttttctgg tcgatgagtg ctgcattctg caaaagcgtt tttttctgcg
     7501 cttctgacag ggttgataat tcgccctgac tgacctgata tttcatctta gccagttcag
     7561 tattctgccc tgccagtgct atctgttctt tttgctgttt aatcagccgt ttataaatat
     7621 cttctgtttt ttccgcttcg gtctttttat gcgctttggg tttatttgcc tggttatttc
     7681 gccaggcatc cagtgagtta ttgatataat tctgtctggc tgtctgatac gcctctccca
     7741 caaagccgag atcatccgca gcatagccca gtcgggcacg ctcacgcgct tcccccttaa
     7801 ggcgggacag agccagttcg cgcttgctgt tattcagtgc ggtctgctgt ttatcatcca
     7861 gggttgcctg tggtagccgt aacggtacat tcaccagccc ctgtcgctgc tgaagtaatt
     7921 cattaccaag cccaagaagg cgattaaact cggtatgctg cccattcatg atcaacaggg
     7981 actgatacgc tttgttttgt tccgccgcct gttgacggat caacgcaacc cgccgctcct
     8041 ccagcccggc aagcacatcc tgaatggatt gcgctttgcc ctgcatttgt gtgagacggg
     8101 actgttcaac tgccagttga tttgttgctt ctgcaagccc ttctgtgact gtttttaccg
     8161 acgtcatgtg gttaatcata aaaccgttat cggttgtcca gcccgggttt gccagcacat
     8221 actgatagcc agcaattttt tcctgtaagg atttaatctt acttttctgc tcgtcaatta
     8281 acctgttttg ctcatcaagt gcctgccgcg tcttttcctc attatctgac gcttcaggaa
     8341 gcgacattgc cgacgttttc tggcgaattt cgtcgatttt tgcggcatac tggcgtgcag
     8401 attctctggc ctgctcctga ttctgataca tcgtgtacca ggccgcggcc cccagcatga
     8461 caagccccgg caccccacca accagtccca gtgctccact taacagacga cttcccactg
     8521 acgtgacagt attcagcgtt gtctgtgctg ctgttctggc cgcaatatta cgggtaagtg
     8581 atgcctgggc agcagccagt ttcgcttctg cggctgcctg cctttcggta ccgcgagcag
     8641 caacaaccgc ctgttgcgca cgataaaccg ccgcacgcgc cctggctgtt gctatctgtg
     8701 tcccccggag ctgcgcttcc gcaagagcca cttcgtttct ggctgcagta attaatccgg
     8761 cagttgcaga tccagcagaa gacgccatat tgccaaaata tcgggctacc ccgacggcaa
     8821 ccagagcacc ggcagcggtt gccacagtgt caatattgcc tgcaatacca ttcagcacac
     8881 cggagagcgt cttcgtcact ccgcttgcct cgttcgcacc accaacccag gccataaagg
     8941 cgttttcaac tttggttgca gaggatgaaa ccgtatcagg cattgccgca tattcatcac
     9001 gtaatgcccc aagctgacta atcagtgcag gaacaacctt atcggcggtc aactttccgt
     9061 tatccgccat ggccttcaga tccttacggg caacccccac tcccgcagcc agcgcacgaa
     9121 taacacgatc gccgttctca ttcaccgagt taaactcttc accgcgcagc actccctgcg
     9181 ccagtgcctg actgaactgc gtgatcaccg aactggcttc tgctgtactg gcaccggata
     9241 atttcaggcc cgtggagatc gcctcggtga ctttcagtac ctcctcagaa ctgtaaccat
     9301 actcccgcat ggaagctgca gaacgggcaa aaaggctggc gttatcagaa aacgccgtcc
     9361 ccgttctctg gctgatcgcc attaattcac gctgtgatgc ctgaaaatca tcactggact
     9421 gtgaggcctg cttcagacgg gcatttactg aattccactc atcggcgaaa gaaataagat
     9481 gaccggtagc aaaagccccg gcaaatgccc ccgccatatt cagtgccgaa gatttagctg
     9541 tatttatctg atccgtcact tctgccagtg cacgccgcat ttcacgggat gcagcagcgg
     9601 actgccggcc tccgttctgc atggtacggt agtaatcctg ccccatacgc gaagcccggg
     9661 agatctctga ctggaatgac cgggaatttg ccgagatttt aataatcagt tcacgtaatg
     9721 tcgccacact cattctccgg acgaaaaaaa accgccgaag cggttatgtt gactcactga
     9781 gacactatta aaagcgcgtt ttccagtccg gcaaatggat ctgatacgcc ttctgtctgc
     9841 tccttctccc actgaagaag cgcatcattc agtggcactt tgaccccctg cgcaccgtaa
     9901 acagctgaaa caatctgggc agcccggata tcagcccgtt cgtcccccag cgggctgaac
     9961 ctgtcaaatt ctgcccacat catgatttct gatgcggaca tttcccggcg taactctgac
    10021 aatgtgcgcc ccatcctgag cgccagcatc atcagaaaac gcatccccgg aagcgctact
    10081 tttttttaac ctcgccggca tcactgatca gttccagaga ctgccgaaga agccgcgcat
    10141 gcaccgggcc atacacggca atcacctgtt cacgatcatc ctctgaaaat acgggttgca
    10201 gtccggtatc acacagaaca tcaatgaaca gttcaacatc tgcctccaga tttcggcggg
    10261 cgcgctccgc aacggataac ggtgtctcat catcttttgc tttaacgatc tcctgccagc
    10321 gcaaccaggc ttctgcagaa ggttcccgta acacaaccgt tgctccctcc cattcaggca
    10381 catcaacggt tttatggcga aaccccgaca tcgttgccag tgccagatta cggatatttt
    10441 tagtcatcac atccatcctc attaactgac ggttacagtg caggaagtgg aggtcacttt
    10501 gttaaccggg cttgctgaat cagaaatctc gcaggtatat gcacccgcat caccggatac
    10561 tgccgatgcc ttactgaacg ttgccgccgt ctgtccggaa acaggagaac cacctttctt
    10621 ccagacataa gaataaggcg gcacaccacc cgcagcctca accgccattt caagtttcgc
    10681 tccggaagca acccgcagcg tgctttttaa atcggccttc actttcagcg gctctgtcgt
    10741 cagcacaggt ttacctttca ggcgcaagga aaacgttgca gccacaacac cattggttcc
    10801 tgcagaccag gtatgctgac gcacctctgc cataaaggta aatccgttgc ctgacggaaa
    10861 aataacttta aagccatacg tggtgtcatt gtcataggca ctgcgcaacg cgttctgggc
    10921 agcattcaga taaaagttgc ctgacatgga aatctctgac gcggcaccaa gaccgttaat
    10981 attttcctgc tcaacagaac acagcgtggt gacatcaata tcctgctttt gtcctgcggt
    11041 aaactgcacc tctttgattg tacagctcag gccaagatag ctggcagaat ccagggtttc
    11101 tgctgttacc ggtgcagacg aaatcataat tttcgtcagt tgcgaacgct caaaattaga
    11161 ggacatactc gtctcctgaa aataaaaaaa cccgccagcg gcgggtgggt aaaatcatta
    11221 atgacctcag gctattacct gaaattcaag cgtggctctg ctcagacggg agtcaggatc
    11281 ataaccctgc gttttagaaa taacggaggg tgccagtttt cttaccgcat caagcgcctg
    11341 ctcacgaata ttatctgcgt catcaggtac tgtcgcccag acatcgatct gcacggtaat
    11401 tctggattca gcctgcccat caagcacatc agatgccgtg tcagacacca cagaaaacac
    11461 cagccacggc ggagataccg caggctttcc ctccgtcagt gggaccacat aaggataaac
    11521 ctgtcctccg gccagctgag acaacaggga atacagtgtg gcctctctca tttacttaag
    11581 acctcatcaa tagcctgatt cattcgctgt atggcaatcc gtgctgccag ttcctctgtc
    11641 gtatcgaaag ccgggcgaat gaatggatgc gcgggcatgt ttatcgttcc cagctccaca
    11701 aagcgccagt aaaatgcatt tcggggatcg ctggctttca tgctgttatc actgtttccg
    11761 gttcgcaggt tccgtccgcg aatgtggaca cccgagataa tttccccccg acgctttgaa
    11821 cgctgagtga gaacaaccac atttttcttc agtttcccgg ttcgctccgg cgcacgttca
    11881 acaactgcat cccgcataac ttcagcaccg gcacgggtgg catcgcgcag aaccttattg
    11941 ttttctgccc tgctgagcgt ctccaaatcc cgtgcaatat ccgccaggcc ggaaaaatca
    12001 agactgaaat ccatcacaca ttccccttca ggctgcagag tatttcaagc cgggtagcgc
    12061 gtgcatccgg tattggtgga ccttctatac ccagaatggc ccctttaaat gcaccggtca
    12121 gcactttcag acgtgaagtc gctgtcacat cgcgccggaa tctcatccag actctgaccg
    12181 tagcctgagc ggtttctgct ccgcctgaga ttatctccct cccgctgata cccttaactt
    12241 ctgcccatac ggtagctccc tccgtcaccg tctccaccgg atgccctgac ggagagcggg
    12301 cggtggtgac attcagaata attacgcgat cacgtaatct gcccgcctgc atgtctcctc
    12361 ctacaaagga ataaaacgat aaggctccag cagagaagaa aaaccaaacg ggactggtgc
    12421 cttgctgaca tctgaggaat tttcccggtt ttcgtaccag tgcccgacca gcaacatgag
    12481 cgccagcaaa acatcatcag ctataagcac cccttcagga tcaccttccg gcaccgtctc
    12541 ctcataaagc ttacggttga taaaattttc tgccttgcgg caggcagccc ggaaatacag
    12601 catcagtaac tcatcatcag ttgcatcatc tgtatcaata cggcactgcg ccctgagttt
    12661 ttccactatt gctgccatca gaaactcctg cccgcaacac tgtgcgggca taaaaaaacc
    12721 gcgtcggcgc ggtctgtaac tgaacaacga gtggttattt gccagtgagc gccttgatgg
    12781 ctgccacatc ttccagcaca cagtcaaaac gatggaaagc cagaaatgcc acctgatcaa
    12841 actcagcata acgctcaacc agacgtttca gttccatata agtaacgcgg cgaatgataa
    12901 agcggttgaa atcccccagg aaaatgaatt tttttccggt accaatcccg tcaatagcct
    12961 gatcaataac ataagggatc cccagcacag tagccggcgt accgcctgca atatccggca
    13021 gccataacgg gcgtttctgt ccatcctcca tctcttcaat agtctgcaat gtggcatcat
    13081 tgaatgccca gcggtatttc ggcccaccac gatatgccgg atcaatggca tgtttcaggg
    13141 cattcatttc tttccaggtg aaagcggcag aggctacagt ctggatggtt cccgtcaccg
    13201 acgctgccag cccttttggc tgtaacggtg atcccgttcc ggtcccctga accagatatt
    13261 ttgcctctcc acgaccaata cgctgggcaa tacggtttgc cagataagat tcaatatcca
    13321 ccccactgtc ctggagcagc tcattggaca cacgaattat ttttgatgac agctttttag
    13381 cccccagaat agcggtcccg aacgtcacat cctgttccgt tgcggctgta ttttccgcca
    13441 gcagttcgcc ctcttcagtc gtgccatcag acgttgacca ggtgatatcc tgcccggttg
    13501 atgtggtcag aagttgcgcc acactggcaa tcccgccata agccttcatg gtgtcaatga
    13561 ttttgttacg catctgcgtg ggcaccgtat atccgccctg agaatccgtt gttacactct
    13621 gagcccgcag ttcacgcatc agattacgct cttcagcatt cagttctgca aatccggcac
    13681 gcagaaaacg gttaaatgcc gcagcgcgct tctcttccac cgcctttttc ccgttctccg
    13741 cctcattatt ctggcgctct tccggcccgg actcatccac atatgcctga tcctgacggc
    13801 gcaactcttc ttcacgggcg atttgctcat ccagcgcatc cagctcagct ttcgccctgt
    13861 tccactctgc ccgttgctca tcagtccatg cgttatcacc aattttttca tgcagtgcac
    13921 gcatatcctt tgcaatggtg tttcgttttt gcttcatctc atgaagtttc atcgtcagta
    13981 gtatccttat gcattaagaa gggtcaaaag acgctcacgc gccattcgtt cgttaacagc
    14041 tttcttcagc gcaccactcg cccgcgcttc ctgccaggct ttcattgagc ggacaccaga
    14101 gtctgcgtcc tgataggccg gatatgtcac cgggctgacg tcatacagac gagaaatgcg
    14161 cgtgatttcc cggataacaa tcccctcgtc gtcttcatac cagctctctc cgtcacgggc
    14221 gacacgaaac gcgaacgagg actgattaat gtcaccacgc aacattggag atagcaccag
    14281 gtcacaaata gtcggagtat ccggtgcaac aatgtcataa cgtaaaccgc gttcatccac
    14341 cgacaatgac aacgtgccgg cagaacttcg tccgagaatg aaattaggat catgattaaa
    14401 caagccacgt acatcatcat tcagtacatc atcaaaagcc cccggcttga tgatttcacg
    14461 aaatccccac agaggttctg aacgactgtt aaataccgag ccatagccca gaatgtgggt
    14521 cggggcatta tcatattgtt ccgcccgcac ctccccgctg taacagcgcg tttcacggtc
    14581 attcatcgtt cttttcctct ttgcctttcg tatctttaaa gttattcaac ggatttgctg
    14641 catttacgct gaccagcatt tcatccagac cgtcaaccgg gttcatatcc tcaaatgccc
    14701 tggcttcatt ccgactcatc cagccatctg taatggcaaa gtgatagaac tgcgcacgct
    14761 cctgtggggt cccacggagc aaccccgtga ggttgaaacg aacgtaatac ccggcagccc
    14821 gttctgtacg ggtaaacagg cgacggttaa gctcctgctc ccagttcgca acccagggca
    14881 tcatcgtgta gcgaacaaac tgaatcgcct gctgcgtaat attcgaaaat gtggcttttt
    14941 ccaggtcatt aatcatgtgc gccgggacat taaaaatccc ggcaatcatc gaccggttca
    15001 gcttggtcat atcaatgatc tgagcatcca ccggagaaac tgtcagagcg cggtaatcca
    15061 gttgcgcagg cagcaacatg gttttatttt cctggctgcg aagcgctgtc accgcccgct
    15121 gccacatatt cttgagcctg ctccaactct gttcgttcag ttcatttttc acagaaataa
    15181 tcccggcagg acgggcatta ccgttaaaaa aagcactggt atactgctga ccactcattc
    15241 ccataccaat ggtttcagca tgctgcatga tcggactcag tcccattttc tgattgtttc
    15301 ccagcgccct gatatggatc atgtcgtccg gacttaccgc aaatgcaccc tcttcgttat
    15361 acacaccgta ggtatgacgc cctccggtgt taagtaacgt ggtttcccat ggcatacagc
    15421 attcaagact ggtaacctcg ccacgacgat tacgtttcac ccacgtataa ccattgcccc
    15481 accccagcac atgacgctgc ttcagttccc gccacttata gctggtctgc caggcattcg
    15541 gttcatcatg aacgagccag aacaacgggt gatcgcgtgc cggctgaaca tgctcattcg
    15601 tttttcgcat cacatgcagg ggcatctgag ccacactgga tgaaataaca taaatacagg
    15661 catagacagc agccagcctc atggacgttt ccggactgac atacacatcc cgggcaaaaa
    15721 tattatccgt ctctgcggcc tctccggtta ccggaaccga gggattttcc agaggctcac
    15781 tgcgaaacag agcatcaaga agcatgtttt ctcctcatgg acaccaccag tgcataaagc
    15841 agcaacaaac agccagacag catcagagac gctggcagac ctgcatacag ataaacgcca
    15901 gcagtgagca gaccgaaacc gatcagcccg gtcatatcag taataagctg tttcacagaa
    15961 ttaacaggtc ctcatcagga tcaagcgtgg acagaaagtc attcacgccc ccgccattta
    16021 ccagaaagcg gctcatggct gtaaaaagcg caacagggcc gtcgattttg gcttccggcg
    16081 tggatttatt cgggaagatg ttgtcgtttt tgtccggttt tacagtaacg ttagacatca
    16141 tccagttcat gaccggatga ttgctgtgat ggaaacgccc ggcataaacc agtgattccg
    16201 tttccttcat ggcctctgac agattgcgga ccgtctgcgg aacctccacc agcggtatcc
    16261 cttcttcagc cagtgccaga ctgaactgca ttgcgctcca cgggtcaaat cccagttccc
    16321 tgaggttttc accgccaatc cattccagta agtcactttt tatctgagca tgatcgataa
    16381 catcaccatc cgtcaggatg agcttatcca tctccgccca cttccggtaa agttctgcct
    16441 gctgccgcga gcatcgttcc agccgtcctt ccggaagcca gaatttaaaa tcagcatgaa
    16501 catgtccgtt atcggttcgc cagagttttg ccgccgcaca gatatcaatc ttatgagcaa
    16561 ggtcgacgcc gacccacatg ggatatgttt tcagctcatg ttgtggagca atgtattcgc
    16621 acttctccca cttaatcata tccatccagg cagattcggc agtgacccac acattcatgt
    16681 gtttggtaaa aaaattcacc cgcgcagaga cctgctcctt cgcttttttc gccagacgac
    16741 gcagatcatc ccagcgttta cagatgccca ggccaggatt cgctttctgc cagaccgttt
    16801 catcaaacgg atcatctccc tcatcaagcg tgtaaatgat cgcaaagtaa gagtcgtctt
    16861 ttacagcgcc ctccacgtcg ctgttatagc ctcgcaatac cttgatggcg taatcacgct
    16921 gctcgtaaca aatcccttcc ttgttaaagc cagccgtggt gataccaaat aacagggact
    16981 gcagacgggc accggttgcc gtttccagaa cgtcccacac gtcgcgggtt ttatgtgcat
    17041 gcagctcatc aataatggcg cagtggatgt tcagaccgtc caggttgttt gcatccgagg
    17101 aaagcggttc aaattttgat gcgctctgct cctggtaaat cgccagcttg ttgaaatcaa
    17161 acaaccgccc gagtgtcgac cgggcttttc tgaccatatt tttggcgtct tcaaacacga
    17221 ttctggcctg gtcacgcgtg gttgcggctg aatacacctc agctccgcct tcactatctg
    17281 cccccgtcat atacaggccg atacccgatg acagggttga ttttgcgttt ttacgggcga
    17341 cttcgttgta cgccgtccgg aaccggcgca ccatcaccgg acgtccgctg ccatcgctgc
    17401 gcatgacaac ttccccggtt tcttcattga ccagcggaat gacaaaacca aaaatattaa
    17461 tgaggataaa tacatgccag tccatcaact caatgggctg gcctgccagt gcccctttta
    17521 catgaggcac aaatttgtag aaattcagga tgtgctgcgc acggggttca ctgaaataaa
    17581 tcccccgctt ttcgccgtac ttcagatcat caagaaaacg ctggcaggcc aggcggacaa
    17641 attcgccagc aacaatttct cctgcaacaa cacgttcggc gtagcggatc ccgtcagcca
    17701 cttttgccat cagtctctcg cttttaaaag ctctgccagc ggatcaacat tatccggtcc
    17761 ggcggtattt acttttgccc ggcttgccgg tgacatacca aattctgcaa gcatcgcccg
    17821 gatccgcttc caggcatccg ctttcatcgc agcagccggg tgtgccttaa tcagcacatc
    17881 accgttctgc gttttcgagc ggtaggtgta cccctcaaca tcgagtgttt cgcagtgatg
    17941 ccggtattcg gtataggctt ccaccagtag ctcaagcgca cgcgcatcaa gctgagaaat
    18001 gatcccttcc gcattcagct cttccgccat tcgcctgaac cagtacttcc cctgcgcccc
    18061 taaatgttgc ggaattttag ggagaccttt ttcatccttt ttagcggttt ttttgaggtc
    18121 tttaactggc cgctttgagg ggttgcctcg tatcaaatgc aggcgtggcg gggttttcgg
    18181 gggtccagac ataatcggtt ttacctatca atcatttaat cacattccaa aaaaaagttt
    18241 tcgaacctgc ggcgatgcga ggaaaggtca ggcggcggta ctgagcagcc agggttgcag
    18301 agatttggcc tgcccctccc ctacagatgg gaactgttat caattgatgc gttcgcgcgc
    18361 tgtttttgct ttatgacagg gccagcacag actctgcagg ttactgtctg catccgtgcc
    18421 accatgagct ttcggaatga tgtggtccac agttctggct tcaacggctc tcccattgcg
    18481 caggcagttc tgacacagat gattatcacg cttcagtatg cgcgcacgta tggcatccca
    18541 tttcgagcca tagccacgct ggtggcggct cagtccgcgt tgatgctgta cccatccttc
    18601 gccacgatgt ttatcgcagt aaccagaact gtctgtggtt gtacctgcac atccacgctt
    18661 acggcaggcg cgtgggattc gtgggggcat atgtactcca atgaagaagc caccgacata
    18721 gcctcctcca ttcatagtga aactattttc atctacccag taatgaattc tttgtagagt
    18781 tgtgatcaat acaactcact aatggagagg cttgtccaac acgttggaca agtttcctgt
    18841 ttgatttact ggacactata gaaggacaga atgccttcat cactcgaata acatcaatta
    18901 aggaggttca acatgtttca ttccacaaat catcaggctg taattatggc tgcatcagct
    18961 tgtgccacag accttttccg cttcactttg agcctgattc atttctacct gaccggctcg
    19021 cctctatctt tttaatcccc gctttatcca aattgcattg ccagaatgcc gacaacagac
    19081 tgacattcaa atcctgacta cctccgatag gctgagcatc cacctat
//
LOCUS       DAPQZD010000084        19106 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPQZD010000084 DAPQZD010000000
VERSION     DAPQZD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.084"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..136)
                     /locus_tag="RO454_002889"
     rRNA            complement(<1..136)
                     /locus_tag="RO454_002889"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            617..1309
                     /locus_tag="RO454_002890"
     CDS             617..1309
                     /locus_tag="RO454_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDY0934693.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1332..2759
                     /gene="mdtD"
                     /locus_tag="RO454_002891"
     CDS             1332..2759
                     /gene="mdtD"
                     /locus_tag="RO454_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDY0934694.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2725..3717)
                     /gene="rbsR"
                     /locus_tag="RO454_002892"
     CDS             complement(2725..3717)
                     /gene="rbsR"
                     /locus_tag="RO454_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDY0934695.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3721..4650)
                     /gene="rbsK"
                     /locus_tag="RO454_002893"
     CDS             complement(3721..4650)
                     /gene="rbsK"
                     /locus_tag="RO454_002893"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDY0934696.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4776..5666)
                     /gene="rbsB"
                     /locus_tag="RO454_002894"
     CDS             complement(4776..5666)
                     /gene="rbsB"
                     /locus_tag="RO454_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDY0934697.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5691..6656)
                     /gene="rbsC"
                     /locus_tag="RO454_002895"
     CDS             complement(5691..6656)
                     /gene="rbsC"
                     /locus_tag="RO454_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDY0934698.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6661..8166)
                     /gene="rbsA"
                     /locus_tag="RO454_002896"
     CDS             complement(6661..8166)
                     /gene="rbsA"
                     /locus_tag="RO454_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDY0934699.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(8174..8593)
                     /gene="rbsD"
                     /locus_tag="RO454_002897"
     CDS             complement(8174..8593)
                     /gene="rbsD"
                     /locus_tag="RO454_002897"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDY0934700.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8760..10628)
                     /gene="kup"
                     /locus_tag="RO454_002898"
     CDS             complement(8760..10628)
                     /gene="kup"
                     /locus_tag="RO454_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDY0934701.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10851..12347
                     /gene="ravA"
                     /locus_tag="RO454_002899"
     CDS             10851..12347
                     /gene="ravA"
                     /locus_tag="RO454_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDY0934702.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12341..13792
                     /gene="viaA"
                     /locus_tag="RO454_002900"
     CDS             12341..13792
                     /gene="viaA"
                     /locus_tag="RO454_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDY0934703.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13797..14789)
                     /gene="asnA"
                     /locus_tag="RO454_002901"
     CDS             complement(13797..14789)
                     /gene="asnA"
                     /locus_tag="RO454_002901"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDY0934704.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSKEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14941..15399
                     /gene="asnC"
                     /locus_tag="RO454_002902"
     CDS             14941..15399
                     /gene="asnC"
                     /locus_tag="RO454_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDY0934705.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15489..15932
                     /gene="mioC"
                     /locus_tag="RO454_002903"
     CDS             15489..15932
                     /gene="mioC"
                     /locus_tag="RO454_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDY0934706.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHNIPEDPAEEWLGSWINLLK"
     gene            16311..18200
                     /gene="mnmG"
                     /locus_tag="RO454_002904"
     CDS             16311..18200
                     /gene="mnmG"
                     /locus_tag="RO454_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDY0934707.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            18264..18887
                     /gene="rsmG"
                     /locus_tag="RO454_002905"
     CDS             18264..18887
                     /gene="rsmG"
                     /locus_tag="RO454_002905"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDY0934708.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4787 a         4961 c         4711 g         4647 t
ORIGIN      
        1 ggcagtttcc cagacattac tcacccgtcc gccactcgtc agcgaaacag caagctgttt
       61 cctgttaccg ttcgacttgc atgtgttagg cctgccgcca gcgttcaatc tgagccatga
      121 tcaaactctt caatttaaaa gtttgatgct caaagaatta aacttcgtaa tgaattacgt
      181 gttcactctt gagacttggt attcattttt cgtcttgcga cgtcaagaat ccgtatcttc
      241 gagtgcccac acagattgtc tgataaattg ttaaagagca gtgccgcttc gttttttctt
      301 agcggcgcgg ggtgtgcata atacgctctc ccgctacaga gtcaagcatt tatttttgct
      361 ttctctgccg gaattctcag gagaatcccg ctgacccggc ggcgtgtttg ccgttgttcc
      421 gtgtcagtgg tggcgcatta tagggagtta ttccggcctg acaagaggaa atttaaaata
      481 attttctgac cgcgcatttt ttcagcaaaa gccccgttaa tgtgccataa aacaagcaat
      541 ttgccgtttt tgcaaccgta atcacacttc ctggtggcat actaaggagg aataaaaaga
      601 aaaaggaaga ttagtaatgc ctttaagcgc acaacagtta gctgcacaga aaaacctttc
      661 gtatgttctg gctgaaaagc tggcgcagcg gatcttaaaa ggtgaatatg aacccggcac
      721 cattttgcct ggtgagattg agctgggcga gcaatttgga gtgagtcgta cagcggtacg
      781 cgaagcggtc aaaacgttaa cggcaaaagg gatggtttta ccgcgaccgc gaattggtac
      841 ccgagtcatg ccacaatcga actggaattt tcttgatcag gaattgctta cctggtggat
      901 gacagaagag aactttcatc aggtcatcga tcactttctg gttatgcgta tctgtctgga
      961 gccgcaagct tgcctactgg cagcaacggt tggcaccgcg gaacagaagg cgcatctcaa
     1021 tacgttaatg gccgaaatgg cggcattaaa agagaacttt cgccgtgaac gctggatcga
     1081 agtcgatatg gcctggcatg agcatatcta tgaaatgagc gccaatccgt ttttgacctc
     1141 attcgcctcg ctattccatt cggtttatca cacttacttc acgtcaatta ccagcgacac
     1201 agtgataaag ctggatctgc accaggcgat tgtcgatgcg attatccaaa gcgatggcga
     1261 cgcggcattt aaagcttgtc aggcgctgct acgctcacca gataagtgat aacccgataa
     1321 caggactttg aatgagcgat aaaaagaagc gcagtatggc gggtttgccg tggatcgcgg
     1381 cgatggcctt cttcatgcag gcacttgatg ccactattct gaataccgcc ttacccgcaa
     1441 tcgctcatag ccttaatcgt tctcctctcg cgatgcaatc agccatcatc agttatacgc
     1501 tgacggtggc gatgcttatt ccggtaagcg gatggctagc cgatcgcttc ggtacgcgtc
     1561 gcatttttac ccttgccgtg agtctgttca cgttgggttc tctggcctgc gcactttcca
     1621 actcgctacc acagctggtt gtcttccggg ttattcaggg gataggcggc gcaatgatga
     1681 tgcctgttgc tcggctggcc ttactgcgcg cttatcctcg taatgaactt cttcctgtat
     1741 tgaattttgt cgccatgccg ggtctggtgg ggccaatttt aggccccgtt cttggcggcg
     1801 tgctggtcac ctgggcaacc tggcactgga tatttttaat caatatcccc ataggtattg
     1861 cgggccttct ttacgcgcgc aaacatatgc ccaatttcac caccgcacga cgcagattcg
     1921 ataccactgg ctttttgctg tttggcctca gccttgttct cttctcaagc ggaatagagc
     1981 tattcgggga aaagattgtc gccagctgga ttgccttgac ggtaattgtc accagcatct
     2041 ggttactgct tctctatatt ctccatgcgc gacgcacgcc aaacccatta atttcattag
     2101 atttatttaa aacccgcact ttctcgatcg gtatcgtagg caatattgca acccgtctgg
     2161 ggaccggctg tgtaccgttc cttatgccat tgatgttaca ggtaggattt gggtatcagg
     2221 cgtttattgc tggctgtatg atggcaccga cagcgttagg ttccattatt gcaaaatcga
     2281 tggttaccca agtcttacgt cgtctgggct atcgccatac gttagtgggg atcacggtga
     2341 ttattgggct aatgatcgct cagttctctt tgcaatcacc agcaatggcg atatggatgc
     2401 tgatcttgcc gttgtttata ttagggatgg ctatgtcgac gcaatttacc gcgatgaata
     2461 ccatcacact tgccgatctg accgatgaca acgccagcag cggtaacagt gttctggcgg
     2521 tcacgcagca actgtctatc agtttaggcg ttgctgtaag tgcggccgtc cttcgcgttt
     2581 acgaagggat ggaaggcaca acgacagtcg aacaattcca ctatacgttt atcacgatgg
     2641 gcattattac tgttgcttca gcagcaatgt tcatgctact gaaaacaacc gatggtaata
     2701 atttgatcaa aagacagcgt aaatctaagc cgaaccgcgt tccatcagaa tcggagtaag
     2761 ttgtaatcgt tgttgctgta gggtcggctg ggttatccga tggatgagta catcaatcgc
     2821 cagctccccc agttcatctt tcggttggtg tatagttgtt aatggtggcg tcataaagct
     2881 tgccagttcg atatcgtcat agccaatcac cgcgatatcc tgcggaacct gtaactctgc
     2941 ctgatataac gcctggtaaa cgccaacagc catagcgtca tttccggtaa agacggcctg
     3001 aggacgcagc ggatgtgata acagttggcg catagcgtca aacccgccgt taaattcaaa
     3061 atcaccagtg acttcatagc catcaggaat gttgagaccc gcacgtttca tcgcagcccg
     3121 ataaccttcc aaccgcaggc gcgccggagt tttatccagc ggaccggtaa tacaggcaat
     3181 acgggtatga cctttatcga tcagatattg cgttgctaag tctccgccca gcaacgagtt
     3241 atcctgaata agatcgctgt cgccatcgaa cggagcccag tccatcatca cggtaggcac
     3301 tgtcggataa cgttgcatga tttcacgcga aggctgatgc gtttcggtgc acagtaacag
     3361 caagccatca acgcgttttt gcatcagcgt ttccagatta cgattcatcc gctgttcatc
     3421 gccttcggta ttgcaaagga caagactata accgcgttcg aagcagctgc gttcaacgcc
     3481 acgcaccagt tctgaataga aaggattggt actggcagtg atcaacatgc caatggtatg
     3541 tgtttgattg agtttgaggc tacgcgccag agctgatggc gcgtaattga gttctttaat
     3601 cgccgcttca actttggctg taatcgcttc actgacgaag cgatctttat tgataacgtg
     3661 agaaactgtt gaggtagaaa cgcccgccag gcgggcaaca tctttcattg tagccaagcg
     3721 tcacctctgc ctgtctaaaa atgcgtcgat ctcttcacgc cacggtacgg aaggttgtgc
     3781 gcctttacgt gttacggcaa tcgcagcggc tgcatgggca aaacgaatcg cctctggcaa
     3841 tgttttttct tccagcaacg ccgtgattaa cgcgccgtta aaggtatctc cggcagcaat
     3901 ggtatcgaca gcctgcaccc ggaatccagg aacgcgctga ccttcaccat tcacgctagc
     3961 ccatacacca cgacttccta aagtaatcag tacagtacgg ataccttttt catgcagtac
     4021 ctgcgccgcc ttcgctgcat cttcatcatt ttcaacacga ataccggtga gcttttctgc
     4081 ttccgtttcg tttggcgtaa taatgtccac cagcgccaga agttcgtcag gaagttcgcg
     4141 agccggagcc gggttaagcg caacgatagt cttattttga tgggcgattt tcgccgctgc
     4201 catcacactt tcgagtggtg attccagctg cattaataat gccgacgcgt tggcaatacg
     4261 ctcacgttgc gcttccacca gcgccgggga aagggcagca ttagcgccgg catgaatacc
     4321 gatgacattc tcaccttcgc cattaacaaa aatcagcgcc acacctgttg attcgccttt
     4381 gatcacgctg accggagaaa tatcgatatt atcagtggcg agctgctggc gaacgctctc
     4441 accaatgctg tcatcgccag tgcaggcaat aaacgcgata ttcgcaccgc tacgcccagc
     4501 agccaccgcc tgattcgcgc ctttgccgcc aaatgcaacc tgatagtggt taccggttac
     4561 ggtttcgcct ggagtaggaa aagattgaag attaagaatg tggtcagcat taatgctgcc
     4621 aagaacaacg aggctgcctg cgttttgcat attcgggatg tccataaaat gcgccaccgt
     4681 gttagggtgg cgcgtgccct gcttttcttt atattacacg ttcatctgtc gatgacgtat
     4741 ttatgtcacc atcaggtcat acaacctgat taaaactact gcttaacaac cagtttcaga
     4801 tcaaccggat acttagcctg aactttctct cctttcagca ctttatctgc ggtttcgacg
     4861 cctttcgcgc caatctgatc gggtagctga gcgatagtcg ctgctagttt gccatcattc
     4921 accgcttttt cgccatccgg tgtaccgtca aatccgacga ccatcacatc cgatttaccg
     4981 gcagtttgca gtgcgcgcag cgcgccaagc gccatttcat cattctgcgc gaatacagcc
     5041 tgaacatccg gatgagcggt caacaggttc tgcattacgt tcaagccttt ggtgcgatca
     5101 aaatctgcag gctggctggc aagaacatta aacttgtgag cagcaacggc ctgctggaaa
     5161 ccttcgccac gttcacgggc tgcggatgta ccggcaatgc cttgcagctc gataactttg
     5221 gcaccttcac ccgctttctt cgcgatgtaa tcaccggcga ttttgccgcc cagtacgtta
     5281 tcagatgcga tgtggctcac cacttcacct ttcgttgcct ggcggtcaag agtgataacc
     5341 gggatgttcg cctggttagc catcttcaca gcattaccta ctgcgtcgga gtcggtcggg
     5401 ttaatcagca gaattttggt gccgcggacg gttaagtcct gcacgttcgc cagctctttc
     5461 gccgggttgt tctgggagtc cagcaccacc aggttatagc caagtttatc cgcctctttc
     5521 tgcgcgccat ctttcagcga tacaaagaac gggttgttaa gcgtggagac caccagcgcg
     5581 atggtgtctt ttgccatcgc attcgcactg acggtggcgc ttagcgcaac agcggaaacc
     5641 agggtagcca gttttttcat gttcatattc aagatgtcct gtagtcgtta ttactgcttt
     5701 ttgttgtcta ccagcaccgc cagcaaaatc accaccgctt tgacgatcat ctggtaatag
     5761 gaggaaacac ctaacaaatt caatccatta ttaaggaagc caagaattaa tgcgccgatc
     5821 aacgtcccaa caatgcgacc ttttccgccc gccagactcg taccgcccag aaccaccgca
     5881 gcaatagcat ccagctcata gccagtcccc gccgtgggtt gtgcggagga gagacgcgcc
     5941 acttcaatga tcccggccag cgatgccagc agaccacaaa gagaatagac gatgattttg
     6001 attttattga cgttgatacc agaaagacgc gttgccgctt cgttgccgcc cagcgcgtag
     6061 atgtaacgcc ccagacgcgt gtgatgcagc atgtaccagg ccgcgaggaa gacaatcccc
     6121 atgatccaga ctggcgtcgg tacgcccagc ggacgaccaa taccaaacca gccaaacaga
     6181 tcggcgttct cagtaaagcc ggtattcact gggctaccat tggtataaac catggtcacg
     6241 ccgcgcagta aaagcatcat aaccagcgta gcgataaacg cctggacgcg acctttcgct
     6301 acaatcaccc cagttaccgc accaattgcg gcacctaacg cgagagcggc agcgacagcc
     6361 accagcgcat tgacttcaat gccgacgata gatgcagcaa ctgcgccggt cagcgccaac
     6421 agagaaccta ccgacaagtc gatacccgac gtcaggatca ccagcgtcat cccgaccgcc
     6481 ataatggcgt tcaccgaggt ttgctgaaga atattgaata agttattgat ggtgaaaaag
     6541 ttcgggctta acgtcgagac aatcgcgatc agcaccagca gagcgataag cgatttctgc
     6601 tccatcagcc acgctttcgt gaaataacgg cgaccagaga cagtctgggt tgtcattttt
     6661 ttactcctga ttcacgcgat taagcttgcc cacagcggca gccattaaca cttcttgggt
     6721 ggcctgctca cgagtaaatt ccccgctgag atgcccttca tgcatgacga tgatgcgatc
     6781 gctcatgcct aatacttctg gcatctccga tgacaccaga atgatgctca agccatcggc
     6841 tttgaactgg ttaatcagtt gatagatctc ttttttcgcg ccgacatcta cgccacgggt
     6901 aggctcatcg aggatcaaca ctttagggcg tgtcatcaga ccacgggcaa tcgctacttt
     6961 ttgctgattg ccaccggaaa gcagaccaat tgcctgttcc atcgatggcg ttttcacatt
     7021 aaacagacga atgaaatcac tcacagcctg ctgttcatcg gcatgcttca aactgccgcc
     7081 agcgcggctg aagtagcgca gcgcggtcag cgacatgttc tcttttactg acatgcccag
     7141 cactaaaccg tcacgtttac ggtcttcgga gatataaaca atgccgtttg ccaggccatc
     7201 ctgcggcgaa cgggtaacga cttcatgccc atccagggtg acgtaaccgc ttgtgcgcgg
     7261 cagtgcgccg tagagcactt tcatcagttc ggtacgccca gcgcccatca aaccagagac
     7321 gccaagaatt tcgcctttgc gtaaagtaaa agagacatcg ttaacgccag gtccgcagag
     7381 attatcgact ttcaggcgga tatctcccgg cgctttgtcc aggtgcggat attgatcttc
     7441 cagcttgcgg cccaccatca tctcaatcag agaatcttcg gtcagtgatg ccacttcacg
     7501 ctcagcaata aattgtccgt cacgaaaaac ggtaacgtca tcgcaaatct cgaagatttc
     7561 tttcatgcgg tgggagatat agacaatacc gcggccttgc aatttcagct cgcggatgac
     7621 gcggaacaag gattcggttt cggtatcggt cagcgcatcg gtcggttcat ccataatgat
     7681 gactttcgac tcaaagctca gcactttggc gatttcaacc atttgctggt caccgatgga
     7741 aagatcaccc accagtttgt cgcttttaaa gcgcaggtta agtttagcca gcaatttatc
     7801 cgcttcggca tacatggttt tccagtcaat tttgccaaag cgattaacaa actcacgacc
     7861 gaggaaaatg ttttcggcaa tggttaactg cgggatcagg ttcagttcct ggtggataat
     7921 cccaatcccg gcttcctggg aagatttcgg cccggtaaat gtcgtttctt tccccaacca
     7981 taaaagcgta ccggcatcgc gagtatagat accagtaagc actttcatca tggtggattt
     8041 acccgcgccg ttttcgccca ccagcgccat cacgcggccc ggatagacat ttaacgctgc
     8101 gcccgagagg gcttttacgc ccggaaaggc tttatcgatg cctttaagct gaagtaatgc
     8161 ttccatgacg gcctcagaac gtcacgccag cacagagaat gatattcgca tacggagaac
     8221 attctccgct gcgaattacc gcctgacttt ctgcggtttg ttgtttgaat tgttcatgcg
     8281 tggtgtaacg aatttcaatg gtgtttccct ggtgttgttg cagctgctca aggtgagtga
     8341 gcaacgtttc gtggagttgc ggattatgtt gtttgatctc ttccgcgata atgacagcct
     8401 cgacctgcat ttcatttgtg acgacgccca gcacctgcat aaaagaaggt acaccctggg
     8461 ttaatgccat atcgatacgc gttgtacttt tggggatggg taaaccagca tcacacacca
     8521 ccagcgtatc ggtatgtccc agtcgggaga tcaccgatga aatatcagaa ttaagaacgg
     8581 tgcctttttt catttttttc tccatcagcg aaacgtttcg ctgacccgca gtttaatcct
     8641 gactgagttg ggaaaaccat cgtgacgtta cggaaatgtg atcaacctcg aaacgtttta
     8701 catggtgatt aaccatgaaa acaaaaacgc ccccttatgg agaaaggagg cgtctggcgt
     8761 tagatttcga cctgagtacc cagttcgata actctgtttg gcgggatttc aaattgatcc
     8821 ggtgcccgca gcgcattacg ttgcagcagc aagtacagct tgccgcgcag acgcaaatac
     8881 cacgggcgtt tgccgaggat caacgactca tgcgacataa agaaggaggt ttccatcatc
     8941 cggcagctta atccttccag accgcagcgg tggaaaactt cttccacgtt tggcgtttct
     9001 cgccaaccat aacttgccac cacgcgccag aaagtgggcg acagttgttc aatctgtacc
     9061 cgacggacgt tatggacata tggcgcgtct tcggtgcgca gagttaacag aatcacccgc
     9121 tcatgcaata ccttgttatg tttaaggtta tgcatcagcg caaagggaat gacgttgatt
     9181 gcacgcgaca tatacaccgc ggtcccaggc acgcgaacgg gcggtgattt ctccagcgaa
     9241 gcaatcatcg cttccagaga gttaccatgt tcatgcatcc gccgcagcaa gcggaaacgc
     9301 tcgctcttcc aggtggtcat cacgataaac atcaccgtac cgaggctcaa tggcaaccag
     9361 ccaccggaga gcagtttatc gaggttagcg gtgaacaagg gaatatcgac acaaaggaaa
     9421 gcaatcagga tcagcgcaac aaaatactta ttccagtgcc agttctgacg tgccacggta
     9481 gtcgagagaa tagacgtcag caccatggtt ccggtcaccg caatcccgta cgccgccgcc
     9541 aggttgctgg agtgctcaaa gctgacaatc acaatcacga ccgcgacata gagcatccag
     9601 ttcacaaagg gaatatagat ttgccctgac tccatttcgg aggtgtgaat aatgcgcatc
     9661 ggcgacaaat atcccagacg taccgcctga cgcgtcaatg agaagacgcc agagataacc
     9721 gcctgcgagg caattaccgt cgccagtgcg gcgatgatca gcagcgggat cagcgcccag
     9781 tccggtgcca acaggaagaa cgggttctta atcgcttccg gattctttaa caacagcgct
     9841 ccctggccga agtaattaag ggttaaggaa ggcaatacga cggtgaacca cgccagacga
     9901 atagggaact taccaaagtg ccccatatca gcatacagcg cctcgacccc cgtaatcgac
     9961 agcaccactg cccctaatgc aataaaagaa accgttttgt attcaaggaa gaaatgcacc
    10021 gcccacatcg gattcagtgc atgcagcact tccgggttag caataatgct acgtaacccc
    10081 agccctgcca gaatcaaaaa ccaggtcagc atgatcggcg caaacagctt accgaccata
    10141 gctgtgccat gtttttgaat catgaataat aatgtgagaa cgataattga gaggggaact
    10201 atccaggtat ccagctgcgg ggcgacgatt tccagacctt caatggcgga catcaccgaa
    10261 atagcgggag taatgacgac ttcaccatag aaaaagctgc cgccgattag ccccataatc
    10321 accagcattg atgtggttcg cgccgacgta ttacgcccgg caagcgacat caacgtcagg
    10381 atcccccctt caccggcgtt atctgcccgc atcacgaagg tgagatattt aatggaaacc
    10441 acaaagatta gcagccagaa gatcagcgat aaaaagccaa acacggcatc gcgttcaacg
    10501 ccaaaaccaa actggccgga caaacattca cgaagtgtat ataacgggct ggtaccaata
    10561 tcgccgtaga caactccaat cgccgcgagg gtaatcgcgg gcaatgattg cttattatca
    10621 gtgctcatag actagtcttt cgttgaaata tgaaatgtgt gcttagtccc ttggcccaca
    10681 aaaaagcgca cagtatgcac gattaacggc aaaatcgtac tcctaaatgc ggccacatta
    10741 acctggctca aagaaaaata cccctccttt gagactattt tagccaccag caagaaacgt
    10801 ctatactcgc aatttacgca gaacttttga tgaaaggacg ccacttcatt atggctcacc
    10861 ctcatttatt agcggaaaga atttcccgcc tgagcagttc gttggaaaag gggctttatg
    10921 aacgtagcca cgccatccgc ttgtgtttat tagcggcatt aagtggtgaa agtgtgttcc
    10981 tccttggccc accaggtatt gccaaaagtt tgatcgcccg gcgcttaaaa ttcgcctttc
    11041 agaatgcccg cgcgtttgaa tatctgatga cccgcttctc cacgccggaa gaagtttttg
    11101 gtcccctttc tattcaggcg ctaaaagatg aagggcgcta tgaacgttta accagcggtt
    11161 acctgccgga agctgaaatc gtctttctgg atgagatctg gaaagcgggt ccggcaattc
    11221 ttaatacctt gctcaccgcc attaacgagc gccagttccg caacggcgca ctcgtagaaa
    11281 aaatcccgat gcgtctgctg gtggcggcct ccaacgagct gccggaagca gacagcagtc
    11341 tggaagcgtt atatgaccgc atgctgattc gtctgtggtt agataaagtg caggataaag
    11401 cgaatttccg ctccatgctg accagtcaac aggatgaaaa tgacaatccg gttcctgcct
    11461 ccttgcagat cacagatgaa gaatatgaac gctggcagaa agagattggt gaaattacgc
    11521 tgcccgatca tgtatttgag ctgattttta tgctgcgcca gcaactggat aaattaccgg
    11581 atgcgcctta tgtctcggat cgtcgctgga aaaaagcgat ccgcttattg caggccagcg
    11641 ccttttttag cggtcgcagt gctgttgccc cggttgatct cattttgctg aaagattgcc
    11701 tgtggtatga cgcgcaaagc ctgaatttga tacaacaaca aattgatgta ttgatgaccg
    11761 gtcatgcctg gcaacagcaa gggatgttga cccgcctggg cgcgattgtg caacgtcacc
    11821 tgcaactcca gcagcaacaa agcgataaaa cagccttaac ggtaattcgt ctgggcggca
    11881 ttttcagccg tcgtcagcag tatcaacttc ctgttaacgt tactgcttcc actctgactc
    11941 tgctgctgca aaaaccgtta aaattgcatg atatggaagt ggttcatatc tcctttgagc
    12001 gtagcgcgct ggaacagtgg ctgagtaaag gtggtgagat tcgcggcaaa ctgaacggta
    12061 tcggctttgc ccagaaactg aatctggaag tggatagcgc ccaacatctt gttgtacgcg
    12121 atgtaagttt acaaggcagt acgctggcac ttcccggttc atcggctgaa ggtctgccag
    12181 gtgaaataaa acaacaactg gaagagcttg aaagcgactg gcgcaagcaa cacgctttat
    12241 tcagcgaaca gcaaaaatgt ctgtttatcc ctggcgactg gttaggtcgc attgaagcca
    12301 gcctacagga tgtcggcgca cagattcgcc aggcacaaca atgctaacgc tggatacgct
    12361 taatgtgatg ctggccgtca gcgaagaggg attgatcgaa gagatgatca tcgcgctgct
    12421 ggcctcaccg cagctggcag tcttctttga aaaattccca cgactgaagg cggcaatcac
    12481 tgatgatgtt ccccgctggc gtgaggcgct gcgcagtcgg ctgaaagatg cccgagtccc
    12541 gccggaactc accgaagagg tgatgtgcta tcagcaaagc cagctcctct ccacgccgca
    12601 gtttattgtg cagctaccac agatcctgga cttactgcat cgtctgaatt ccccatgggc
    12661 agaacaagcc cgacagttgg ttgatgctaa cagcacgatc acttcagcgt tacacacgct
    12721 ttttctccag cgttggcgtt taagtctgat cgtgcaagca acgacgttaa atcaacagct
    12781 attagaagaa gaacgcgaac aactgttgag tgaagttcag gaacgcatga cgctgagcgg
    12841 acaacttgaa ccgattctcg cagataacaa taccgcagct ggtcgtctgt gggatatgag
    12901 cgccggtcag cttaaacgtg gcgactatca gttgattgtg aaatacggtg aatttcttaa
    12961 cgaacagccg gaactgaaac gcctggcaga acagctgggg cgttcccggg aagccaaatc
    13021 aataccgcgc aacgatgcgc agatggaaac cttccgcacc ctggtgcgcg aaccggcgac
    13081 ggttcctgag caggttgatg gtctgcaaca aagcgatgat attttacgtc tcctgccgcc
    13141 agaactggcg acactaggga taacagaact ggagtatgag ttttaccgtc ggctggtgga
    13201 aaaacagttg ctcacctatc gcctgcatgg tgagtcgtgg cgtgaaaaag tgatcgaacg
    13261 cccggtggtg cataaagatt acgacgaaca gccgcgcgga ccgtttattg tctgtgtgga
    13321 tacttccggc tcaatgggcg gctttaatga acagtgtgcg aaagcgttct gcctggcctt
    13381 gatgcgcatt gctctcgctg aaaaccggcg ctgctatatt atgctatttt ccaccgagat
    13441 cgtccgttat gagctttcag gcccacaagg catcgaacaa gcaatccgtt ttttaagtca
    13501 gcagtttcgt ggcggcaccg atcttgccag ttgttttcgc gccattatgg aacgcttgca
    13561 aagccgggaa tggtttgatg ccgatgcggt ggtgatttct gattttatcg cccagcggtt
    13621 gcctgacgac gtgacgagta aagtgaaaga gctacagcgg gtacatcagc atcgctttca
    13681 tgccgtggcg atgtctgcac atggcaaacc cggcatcatg cgcattttcg atcatatctg
    13741 gcgctttgat accgggatgc gaagccgcct gctcagacgc tggcggcgat aaattattac
    13801 agcagagaag ggacgctctc gcgaacagct gccggccata caccacactg aacctggccg
    13861 atatgtggca gttgcagcag cagcatggtc aaacgagact gcccgatacc gccgccgatg
    13921 gtctgcggca tttcaccgcg cagcagcgcc tgatgccact ccagctccag gcgatcttcg
    13981 tcaccggtca gcgccagctg atgcttcagc gtgtcggcat ccacgcggat ccccatggaa
    14041 gaaagctcaa acgcatcttc cagtaccggg ttccacacca gaatatcgcc gttcagaccc
    14101 gcatggccca gctctgacgg ggtgctccag tcatcataat ccggtgcgcg cacgtcgtga
    14161 cgatgaccat cactcaactt gccgccaatc ccgacgagga ataccgcgcc aagatctttc
    14221 gcaatcgccc gctcacgccc tttggcatcg agatccggat aacgagacag taactcctgg
    14281 ctgtgtacga agtggatctg atccggcagg aacggtgcca ggccaaactc tttgctaacc
    14341 gcagcttcgg ttgctttaat tcccgcccag atcgcctcta ccgtgctttt cagagtcgag
    14401 aattgacgct cgccgtcgcc cattacgcgt tcccagtccc actggtctac atagaccgag
    14461 tgcaacgggg aaagacggtc ttcatcgggg cgaagggctt tcatgtgcgt gtacagccct
    14521 tcgcccgcgc tgaagtcgtg ttgccctaag gtctgacgtt tccacttcgc cagtgaatga
    14581 accacttcga actgggcatc aggcagagct ttcactttta cctgcaccgc tttttcacag
    14641 cccgacaagt tatcctgcgt gccatccccc acacggctaa gaatcggcgc ctggacttcg
    14701 atcagcccca gacgttcttc cagttgacga gaaaagtgag atttcacgaa gctaatttga
    14761 cgttgtttgg caatgtaagc ggttttcatt ttttatactc ctgcgtcctg ttgcttatga
    14821 ttaagcaaca aaaaccgaca cacatgcaat aatcattcaa taaaaagcct cacgtgcttt
    14881 tgattcatta aaaaagaagg ctaaaataga atgaatcatc aatccgcata agaaaatcct
    14941 atggaaaatt atctgatcga caatctggac cgtggcatcc tggaagcatt aatgggcaat
    15001 gcgcgcaccg cttacgccga actggcgaaa caatttggcg tcagtccggg gacgattcac
    15061 gttcgagtag agaaaatgaa gcaggcgggg atcattaccg gggcgcgtat tgatgtcagc
    15121 ccgaagcagc ttggttatga cgtaggctgc tttatcggca ttatattaaa gagcgccaaa
    15181 gactatcctt ccgcgctggc aaagctggaa agcctcgatg aagtcactga agcctattac
    15241 acaaccggcc actacagcat ctttataaaa gtgatgtgcc gttcgatcga tgctctccag
    15301 catgtactta tcaacaagat ccaaacaatt gatgaaattc agtccaccga gacactgatc
    15361 gtcctgcaga acccgatcat gcgtaccatt aagccctgat cggctttttt aatcccatac
    15421 ttttccacag gtagatccca acgcgttcac agcgtacaat acgccactct taataaaggt
    15481 ggcggtttat ggcagatatc actcttatca gcggcagcac cctcggcggt gccgaatatg
    15541 tagcagaaca cctggctgaa aagctggaag aggcgggttt taccaccgaa acgctgcacg
    15601 gtccgctgtt ggaagattta tctgcctcgg ggatctggct ggttatcagc tcaacccacg
    15661 gtgccggaga tattccggac aacctttctc ctttctatga agcattgcag gaacagaagc
    15721 ccgatctttc tgcggtccgc tttggcgcaa tcggtattgg tagtcgtgaa tatgacacct
    15781 tttgtggggc tatcgataaa atcgaggcag aactcaaaaa ttccggagca aaacagacag
    15841 gcgaaacact gaagatcaac attcttgatc acaacattcc ggaggatccg gcagaagaat
    15901 ggctgggatc gtggattaat ttactcaaat aagtatacag atcgtgcgat ctactgtgga
    15961 taactctgtc aggaagcttg gatcaaacgg tagttatcca aagaacaacg attgttcagt
    16021 ttttgagttg tgtataaccc ctcattctga tcccagctta tacggtccag gatcaccgat
    16081 cattcacagt taatgatcct ttccaggttg ttgatcttaa aagccggatc cttgttatcc
    16141 acagggcagt gcgatcctaa taagagatca caatagaaca gatctctaaa taaatagatc
    16201 ttctttttaa tacccaggat cccaggtctt tctcaagccg acaaagttga gtagaatcca
    16261 cggcccgggc ttcaatccat tttcataccg cgttatgcga ggcaatcacc atgttttatc
    16321 cggatccttt tgacgtcatc atcattggcg ggggtcatgc aggcaccgag gccgcgatgg
    16381 ccgcggcgcg tatgggtcaa cagactctgc ttttgacaca caatatcgac actctggggc
    16441 agatgagctg caacccggcg atcggcggta ttgggaaggg acatctggta aaagaagtgg
    16501 atgcactcgg cggtctgatg gcgaaagcga tcgatcaggc gggtatccag tttaggatac
    16561 taaacgcaag caagggaccg gcggttcgcg ctacccgagc tcaggcagat cgtgtgctct
    16621 accgtcaggc ggtacgtacg gcgctggaga accaaccgaa cctgatgatc ttccagcagg
    16681 cggttgaaga tcttattgtc gaaaacgatc gcgtggtcgg tgccgttacc caaatgggac
    16741 tgaagttccg tgccaaagct gtcgtgctca ccgttgggac gttcctcgac ggtaaaattc
    16801 atatcggtct ggataattac agcggtggcc gtgctggtga tccgccgtcc attccgcttt
    16861 ctcgccgttt gcgtgaactg ccgctgcgcg ttggtcgtct gaaaaccggg acaccaccgc
    16921 gtattgatgc tcgaaccatc gactttagcg tactggcgca acagcatggc gataacccaa
    16981 tgccggtatt ctcgtttatg ggcaatgcgt cccagcatcc ccagcaggtg ccgtgttata
    17041 tcactcatac caacgagaaa acccatgatg tgatccgcag taatctcgat cgtagcccaa
    17101 tgtacgcagg ggtgatcgaa ggtgtcggcc cacgctactg cccgtcgatt gaagacaaag
    17161 tcatgcgctt tgccgacaga aatcagcatc agatcttcct tgaaccggaa gggctgacct
    17221 ctaacgaaat ttatccgaac ggtatctcca ccagcctgcc gttcgatgtg cagatgcaaa
    17281 tcgtccgctc catgcagggg atggaaaacg cgaagatcgt gcgtccgggt tatgccattg
    17341 agtatgactt cttcgatcca cgcgacctga aaccgacgct ggagagcaag tttatccagg
    17401 ggctgttctt tgctggtcag attaacggca ctaccggtta cgaagaagcc gctgcgcaag
    17461 gtttgctggc tggtcttaac gctgcccgtc tgtctgctga caaagaaggt tgggctccgg
    17521 cgcgttctca ggcgtatctc ggcgtactgg ttgatgacct gtgcacttta ggaaccaaag
    17581 aaccgtatcg tatgtttacc tcgcgcgcag aatatcgtct gatgctgcgc gaagataatg
    17641 ctgatctgcg tttgactgaa atcggtcgtg aactgggcct ggtggatgac gaacgttggg
    17701 cgcgctttaa cgagaaactt gagaatatcg agcgtgagcg tcagcgtctg aaatcgacct
    17761 gggtaacccc gtcggcggaa gctgcagccg aagtgaatgc tcacctgact gcgccgcttt
    17821 cccgtgaagc cagtggtgaa gatctgctac gtcgtcctga aatgacttat gaaaaattaa
    17881 ccacgctgac accgtttgcc cctgcgttga cagatgaaca ggctgcggaa caggttgaga
    17941 ttcaggttaa atacgaaggt tatatcgcgc gccagcaaga tgagatcgaa aagcagctgc
    18001 gtaacgagaa taccctgcta ccagcgacgc tggattaccg ccaggtatcc ggtctttcta
    18061 acgaagtgat cgccaaactt aacgatcaca aaccggcctc tattggccag gcttcgcgta
    18121 tttctggcgt cacgcctgcg gctatctcca ttctgctggt gtggctgaaa aaacagggta
    18181 tgctgcgtcg tagcgcataa cgcattaaaa atgcctggta agcacccgct taccaggcaa
    18241 cgcatcaaga acaggtaatc accgtgctca acaaactctc cttactgctg aaagacgcag
    18301 gtatttcgct taccgatcac cagaaaaacc agcttattgc ctacgtgaat atgctgcata
    18361 aatggaataa agcgtacaac ctgacttcgg tccgcgatcc taatgagatg ctggtacgcc
    18421 atattctcga tagcattgtg gtggcaccgt atctgcaagg tgaacggttt atcgatgtcg
    18481 gcaccggacc gggactgcct ggcattccac tctctatcgt gcgtcctgaa gctcatttca
    18541 cgttgttgga tagccttggt aaacgcgtgc gtttccttcg tcaggtgcaa catgagctta
    18601 aactggagaa tattgagcca gtacagagca gggtagaaga gtttccgtca gagccgccat
    18661 ttgatggcgt aattagccgc gcttttgcct ctctgaacga tatggtgagc tggtgccacc
    18721 atcttcctgg tgagcaaggc cgtttctacg cgctgaaagg gcaaatgccg gaagatgaaa
    18781 tcgctttgtt gcccgaagaa tatcaggtcg aatcagtggt taaacttcag gttccagccc
    18841 tggatggcga acgtcatctg gtggtgatta aagcaaataa aatttaattt ttatcaaaaa
    18901 aatcataaaa aattgaccgg ttagactgtt aacaacaacc aggttttcta ctgatataac
    18961 tggttacatt taacgccacg ttcactcttt tgtatcaaca agataacgtg gctttttttg
    19021 gtaagcagaa aataagtcat aagtgaaaat atcagtctgc taaaaatcgg cgctaagaat
    19081 catcattgac tgttaaaaca ttatta
//
LOCUS       DAPQZD010000085        18787 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPQZD010000085 DAPQZD010000000
VERSION     DAPQZD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.085"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(177..332)
                     /locus_tag="RO454_002906"
     CDS             complement(177..332)
                     /locus_tag="RO454_002906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934709.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAAGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            complement(764..1697)
                     /locus_tag="RO454_002907"
                     /pseudo
     CDS             complement(764..1697)
                     /locus_tag="RO454_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(1690..2085)
                     /locus_tag="RO454_002908"
     CDS             complement(1690..2085)
                     /locus_tag="RO454_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDY0934710.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKSQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
     gene            complement(2154..2999)
                     /locus_tag="RO454_002909"
     CDS             complement(2154..2999)
                     /locus_tag="RO454_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDY0934711.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            complement(3084..3281)
                     /locus_tag="RO454_002910"
     CDS             complement(3084..3281)
                     /locus_tag="RO454_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDY0934712.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            complement(3298..3786)
                     /locus_tag="RO454_002911"
     CDS             complement(3298..3786)
                     /locus_tag="RO454_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934713.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASTECLPDLPTF
                     DW"
     gene            complement(3783..4160)
                     /locus_tag="RO454_002912"
     CDS             complement(3783..4160)
                     /locus_tag="RO454_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDY0934714.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            complement(4249..4617)
                     /locus_tag="RO454_002913"
     CDS             complement(4249..4617)
                     /locus_tag="RO454_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDY0934715.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCDYVYLAVYPTPEMKN"
     gene            complement(4667..5311)
                     /locus_tag="RO454_002914"
     CDS             complement(4667..5311)
                     /locus_tag="RO454_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDY0934716.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            complement(5330..5551)
                     /locus_tag="RO454_002915"
     CDS             complement(5330..5551)
                     /locus_tag="RO454_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDY0934717.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(5614..6090)
                     /locus_tag="RO454_002916"
     CDS             complement(5614..6090)
                     /locus_tag="RO454_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDY0934718.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(6106..6591)
                     /locus_tag="RO454_002917"
     CDS             complement(6106..6591)
                     /locus_tag="RO454_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDY0934719.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(6646..7464)
                     /locus_tag="RO454_002918"
     CDS             complement(6646..7464)
                     /locus_tag="RO454_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDY0934720.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(7565..7798)
                     /locus_tag="RO454_002919"
     CDS             complement(7565..7798)
                     /locus_tag="RO454_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDY0934721.1"
                     /translation="MLGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEADAGKQLNAWLASGGLLRQ"
     gene            complement(7877..8332)
                     /locus_tag="RO454_002920"
     CDS             complement(7877..8332)
                     /locus_tag="RO454_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934722.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(8408..9535)
                     /locus_tag="RO454_002921"
                     /pseudo
     CDS             complement(8408..9535)
                     /locus_tag="RO454_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9630..12752)
                     /gene="ag43"
                     /locus_tag="RO454_002922"
     CDS             complement(9630..12752)
                     /gene="ag43"
                     /locus_tag="RO454_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDY0934723.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASS
                     DNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGNSAEGY
                     NGQATLNVTF"
     gene            complement(13086..13958)
                     /locus_tag="RO454_002923"
     CDS             complement(13086..13958)
                     /locus_tag="RO454_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDY0934724.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            15290..16780
                     /locus_tag="RO454_002924"
     CDS             15290..16780
                     /locus_tag="RO454_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDY0934725.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(16901..17473)
                     /locus_tag="RO454_002925"
     CDS             complement(16901..17473)
                     /locus_tag="RO454_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934726.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(17559..17765)
                     /locus_tag="RO454_002926"
     CDS             complement(17559..17765)
                     /locus_tag="RO454_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDY0934727.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
     gene            18713..>18787
                     /locus_tag="RO454_002927"
     CDS             18713..>18787
                     /locus_tag="RO454_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDY0934728.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSAVD"
BASE COUNT         4371 a         4684 c         4896 g         4836 t
ORIGIN      
        1 tccggttgag ctggagtcac gtatgccagc gagtgctttc ttccgggcct tccttcactt
       61 cagagaaaga gcctatggaa aagaggcaat catcaggctc tttttgagtc atatgcgccg
      121 ataatcaact gtcttgaacg taaaaattat ggagcaacat gatgctacga ttcatttcac
      181 tcttcgttct ttctgagcat tgcgttcagg atggtcagca atttacgcat acagtcaacc
      241 agcgccactt ttttggtttt tccggctgct ggcagatgca tgtaaaacgc ctctttcacc
      301 ggattaaacc aggttaccac aagcgctgcc atgtgcaggg ctgctcttgc ctccgtacgc
      361 gcactggcgt cgttcggcca gcagatatgt gtgggataac agggatgtta tgtgaactga
      421 actgtgtttt gttagcgggg gatgtttatt gatgattcgg gagtgtttaa gacctgatgg
      481 cgttatgcac ccaagtttat tttatataaa gatccaaaag caaaaacccg ccttgtgggc
      541 gggttcttaa gaattagtgg tgcccggaat cggaatgaaa ctgattgtat aacaaattgt
      601 tttatataga ttattttgta tttgttgttt ttgaggccac caatgaggcc cccatgtgca
      661 attcgaattc ttttgacgct ataatccctc ttttttgtgc tttgctgtaa tcatttggtc
      721 tggttcgata tgctgaattg tggaatcgta cttaacgcgt ttgttagctg gtcagaacac
      781 ttagcgctgt ttgtctgaaa ctggaatagg cttcttccca gttaatctgt gtattaccaa
      841 ataccatcgg tttaacaaat cgttgaaagc gcaatttgtg tacaggataa tcccggagtg
      901 catccagccc tttcttaagt tcatcaactg gtgagctaca aaattgtggg tactggttac
      961 catagctgaa tacatatctg aacaaaattt ttcaatacag gaacattcac acctttttcc
     1021 ctgacaatgc agtagttgtc gtaaatgtga cgtaccagaa actcatcatc ctgttgttcg
     1081 ggattacgaa gatgtgctgc tgttcttcgc atcatggaga tgagcttttc agcctgagtg
     1141 gataaaatgg tggcacaggg gaatgctgtt acaggtgtac ctttacccgt taactccgtg
     1201 acaagggaat aaatatctct gctttccgga tgttccagca gagtgctttc catcaactcc
     1261 agcttaataa atggacgaag acaggggact tgtgcaaatg tctggggata acgtaccgag
     1321 atttcgttat aacgatactc atcacgtgtt atacgggcgt tttcattatc caagctgaaa
     1381 atgtcagaat tatgaataac atccgtgatt atttgcacga tttccttgcg aatttttctt
     1441 ctcttgtctc tgccattttg cataaagttc tctgttggca cgagctttat atcaatatct
     1501 tctgacattc tgtttagcga gatacctgct tttgataatg cggtacctcc tgcaaacacg
     1561 agagtatgtg tttctggttt tatttctttt aataatctta aaagatcaac cacaaaataa
     1621 tctttttcga cgatagcgac agattcaata tccagtgcat cggcaacgtc aggaaataat
     1681 gatgttaatt caggcattga ttttactttt ccctactatt aacattcttt taaatcgaga
     1741 aggtgttttt atccccagag atacaggaat ttgtgtggac tgacttttat tgtagcattc
     1801 cgttgctcct gccagacggt atctgatatt caggcgctcc agggcctctg taattactgc
     1861 agcagagccc ccaggcgctg cgggcatgat tttcccagtg atgatgttac gacgggcttt
     1921 ggtgtaaaca ccatagccca ctttcatcaa cttaccttcg tttaccagtg tacgaagagc
     1981 tctgccaacc tgatcatagc cagcgatatc cctgaaatca tcccgggtga acacataacg
     2041 ttttgaacgg ttcagtcggg actgaatacg gtcttttgta gtcatgtttc ctccgaaaaa
     2101 ttttcacaat gatagcaaaa atatgacatg tttaatagcg gggcaaggcc acattacctg
     2161 tctgctaata catttggata gtgcctggca attatgtcat ttagcctgtc aaccagttct
     2221 ggtttcctga acgtgatgtg tgcggagccc ttcttaaagt atctgatgct gaacatctca
     2281 tcttcatagc acgctttgcc ctgaacggag cggatgtgat catccagccg aatgatgata
     2341 ttttcccggc tgtcggggat cggtttgccg ctgaacagat gcagcattct ttccaggtca
     2401 gcgagtcgat ctgcctgttg cccggtgata agatgcaatc cccatcggtc ccatctcacc
     2461 agattgttga cgataatttt attgccaaat ttacaggggc aattggtttt gtaattccag
     2521 ctcagccctc tgaagaggtt gatcacacct cgctcaaaca cgtcatcctt attctggtgc
     2581 agttgttcaa acgtactcag gatattcgcc tcgctgattt ccggaaagtt atcgtactcc
     2641 agtgagcggt accatgtatc atgggcctgc gcatccatta atgacagcat cccggaatgt
     2701 tgcatcaggt cacgccagat ttcgcgatcg agattgcggg tgatggcttt cattgcggtt
     2761 tccggtttct ccatgagcca gcagccgcag cgaaaatcct gtttcatcgc ccagtccagg
     2821 gcggttttac ctccaatact ttctgtcagt actgagattt ctgccagttg ccggataagt
     2881 gtgtcgattt gcagcagcgc actctttcgt acagccagaa tgtggcggat actggttgag
     2941 cagataacgt ccgtatgtcc gttgaccagt tccgggcggg agattgtgat atctgacatc
     3001 atcgtttttc cttttggttt ataaacgaaa acgccaaccg tattcaggct ggcgtcagtg
     3061 gaatgaagtc tggtgagtaa gactcaccgg ttctgttgca gaagctgcag ttggcgcagg
     3121 gtacggatat cttccctggc ctgtttaatg cagggcagca gtgctgctga atccgtttta
     3181 tcctcatcgt tgtcgaagat aatccctgat tcgcagtcaa tattgtcctg cagccacgca
     3241 atcagaatat acagcgctgt ttccgtggtt aatgatttca tgttgtgaat ttccgggtta
     3301 ccagtcgaaa gtgggtaaat ccggcaggca ttcagtgctg gcatccagat gaatgagact
     3361 gacgccataa cgtcgggtaa gtgtgacaac cagacggcgg aacgtttttg acagccccag
     3421 gcgtttaagc cgcagaaccg ggtatgacca cgcatcggta cgtaacagat atccgctacc
     3481 tgtaaaatga atccattctg attcgccggt gtcattcgtc tgatgcgaca gcgagtacag
     3541 ccaggcgttg tctgtttccg tgatatgcgc ggtactacag cgtataccgc tcagggcagc
     3601 agaggggggt atgctgtctg ttgaggtccg gtcagattca tcaacaacac gaagggagta
     3661 gccattatcg cagaccacgt taatcagttc ggcaagctca acaccatcga cgtcaacgac
     3721 aattcgcccc atgttcagtg cctgtacgtt aacgctgtca gcttccagcg tcagggccag
     3781 tttcatcgtt ttgcctccgg atgcttgccc tgggtaatgt tatttaccat tctgtaattg
     3841 ttccgggtca tcaggccggt cgccctgcga gcccggagaa tatcaatgct gttgattaac
     3901 tgcgacgggg ctcctgcgct gaatcccggc tggtcggtac gtacctgcgc gtatttttca
     3961 acgagaaagt tcaccgcgtc acacagtgaa atccctgcct caatatgctg ctcaatcaca
     4021 cgttcatcag cgaacggtgt gtcattcagc gtgaggccat agtgctggtc cagcagtcgg
     4081 gtgagcagtg tctgccagat ggtgacggga gacgggcagc gcgatgccgc ccgtacgtgt
     4141 gtgtcgggta atgttttcat actgaagatt ttcctgatat gcagatataa aaatggaaaa
     4201 gtggcgtggt gaaaacacca ggccgtagca gaaggctatt ctggagagtt aatttttcat
     4261 ttcgggcgtc ggataaacag ccagataaac gtaatcacag gagcccaggg tatcggcttc
     4321 gcaggtcagt cctttcgcat acagtgtgac ggtatgctga tggcggggat tcagttcacc
     4381 gctggtgagc atgagttccg gttgtttcat cagcagcgga aaggcctggt ccggatggta
     4441 cgcatccgca tcgctgaacc ggcctctgat accggcgcgg tcggcaaggt aatgcaaccg
     4501 gttaccctcc tgcaccagac aggccccgaa acagggcgtc acggtgcagg gcagccccca
     4561 ccaggggagg tctttgttgt cgtcgggaag cgttgttccg gggagtgtgt ccgacatgat
     4621 aaaatcctca cagaaaatcg gtgaatgata gtttaacgat gacgggtcag ccggcgcgca
     4681 gaccggcttc ggtgaaggta tattcgttga tgagaagggc ctcatccaca gcggtgtctg
     4741 aggtcagcca gtcgtacttg ttttccagct ggcggtacag ccagaaggcc aggtcgcgca
     4801 gggcttcgct gacagcggct tccgcatcgg ttgtcattgc ctgaccagtc ggactgttcc
     4861 gggtgacggt gatatccatg ctgtacggat ggcagtaacg tccccggtga ctgatttcag
     4921 cctgaagctg ccagaagtta cgtttctgca ccgcctgcag ggtatccgcg atacgatgaa
     4981 gctcacggtc ctgaggggca tattccctga ttttccgggg tgtcgctggc tgccagtgcc
     5041 agcgcccttc aaaacaggct ccgtccccct ggctgcagaa tccgctgaac cagatgcacg
     5101 gctcttccat aaacctgcca ttgctgagcc ggaaaacgcg ctggcggagg tcaatcccga
     5161 aaatgccaca gacctccctg aagtcttcat acacgttttc ataccagttg ctgtcagcat
     5221 gatgctgacg gtaccagtcg cgggcttttc cccgtgccgg gcaggaaagt tcatccagcg
     5281 tatacactgt gatggtgata atgctgctca tctgaatgcc cgtcctgatt cagttcaggg
     5341 tgacgctcat cagccggaca tacgggccaa aactgtcctt acgacgttca gcaaacacgg
     5401 ccaacacacc gggaatatcc tgcacttcac gaccggtata cgcttcagtg ctgccgtgcc
     5461 agcggtattt gccggtacag aacgggaaaa gacgggatgt cggatgttgt tggtgaatac
     5521 gcatggcttc accacgagtg ataattttca taatgggata cctctgaaga cagaagataa
     5581 aagtgaaaac aggtgtgatg tggttgtgac gggttaaagc agaccatgtt cggcaaagga
     5641 gaaaacctgg ttgccaccga ctatcagatg gtccggcacc cggatatcca ccaggcccag
     5701 tgcctgtacc agacgttcgg tgataagccg gtctgcctta ctgggtgtga cttcaccgga
     5761 cgggtgattg tgcgccagta ccacggcagc ggcattgtgg tacagggcgc gtttaatcac
     5821 ttcccgggga tggacttccg tgcggttgat ggtgccggtg aagagggttt caccggcaat
     5881 cagctgattc tggttattca gatacagcac ccggaactct tcacgctcca gtcccgccat
     5941 gttcagaatc agccattccc gtgccgcacg ggtggaggtg aaggccacac cgggttcatg
     6001 aagatggcgg tccagggttt tcagagcccg cagaatgaga ctgcgctcgc cgggcgtcat
     6061 ttctccgggc agaaaggaaa gctgctgcat tgtccctgcc tccattcagt cgatgatgcg
     6121 cataatggcg ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt aataatggac
     6181 cgtcatggca taacactccg tacgacaggc gtgatgactg tacgtcatca gacaggcagc
     6241 aatgccagcg gcttccgggc tcatttcagc gcggttgccg ttcatggcat tgaacagtac
     6301 ccatgtttcg tcatcatcgt catccggttc gggggccata aatgccccgc cgttgttcag
     6361 ggtgtacaga ttccagatac caccgcagta gtcttcgcac agacggtcca tccagccgaa
     6421 aacacggggc tccagggcca cccactgtgg aatgaggcca aaatgctgcg gccagaagct
     6481 gatgcgctgt tcatcaggga ctatggtggc aaccaactga ggctggtcat tccctgatgc
     6541 agcggttacg gaaacagaag atgtggtgga attatgtgaa atggttttca tgaaatgact
     6601 ccttaaagga caaataaatg cgtggttttc agggtaatgg ctgaatcagc gcaggctctc
     6661 cagcagggtt tccgccatca cccacaatgc gcggttgagc ttaatgtcag tgtcaatgct
     6721 gtgaatggca cgggtatgga tacgttttcc tttagcgctg cgacctgaaa ttccgccttt
     6781 cagcatattc tcctgaatgg tctgataagc actccacagg tccttaccgt aatcctcccg
     6841 gcgtcgtggc gtcagaatgt cggcggtggt gacgggctga tgttcgtcac cataacggta
     6901 agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc ggcagaatca gcgactgcat
     6961 ggcatcacgc ttttcctcaa tccggtcaaa cacgcccact acctcgtaag ccccttcaat
     7021 gactctgtcc accacatttc cccggtgcgg aacacgcact tcccccagag actgaccaca
     7081 gacgcacccg ttctggcaga cgaacctgaa gtaacccggc agcatctggt agctggaggt
     7141 accgtcatga gagttgagca gaataatttc agggacatgt tctccgttta tctctccggc
     7201 ccgccgcaga cgcagcatgt gttttgtgta tccccggcgg cccgggtcgc gcacacgggt
     7261 ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt
     7321 ggggatgtac gtatagttct ggcttcggga cgtgtgttta tcttccccga aaatccccgg
     7381 cacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggttcgcata
     7441 accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga tttactggtg
     7501 taataaatga aaaaagccgc gtctcccgga gaagacgcgg cctgatggat gaaatcaatg
     7561 aggtttattg tctgagaagc cctccactgg cgagccacgc attaagctgt tttccggcat
     7621 cagcttcaga gttccggcag cgccagcgtc gctgaccttc cgcgttgcgg ataacccgac
     7681 ggaaatgcat tcccgggtca tcctggataa acacattgcg gtaagcggtt atgacagcaa
     7741 ccgcctgccc gtatgagaaa gatccttcag ccaggacata ctctgtgtaa cccagcatat
     7801 ctgttttctc ctgataaata aaaagcctct gccacgcgtt gtgacagagg ctgaagcatg
     7861 aaactgactt cagggatcag ttaacgtttt ttccggaaac ggtaatcagc agtggatggt
     7921 agtcctgggg atcgaaaacc gataacggca gactgacacg atggccgtta ctttcttcag
     7981 ttgctttaat gatttcggtt gtggcgacat tttccacgca ctccgtttcc agaaatgcgt
     8041 ctgtggttcg cgtggcatta ctgtcaccaa aggcttccgt ttccattttt ctggtcacca
     8101 gcgtctgacc atatttgtct ttgagttgca gagtgatggt gagggggcca aatccttcat
     8161 cgtttccgcc attatccagc cggaactggt aagcacaaat atttcccggg agccatatcg
     8221 tatctgtatt gcgtatactg atgtaacgtt gatcctgtgc ccggagtggg gcagaccacg
     8281 ttaaccccag aatgaaggcg gtaatcatgc aggttttgaa caggtgaatc atggtattta
     8341 cctctctgag tcatgacgat gacactgaca aatcaggtga taaaacgtaa aaggcgcaga
     8401 atagccgtta tgccggtaac tccgggggta atgtttcttc cagtcggtta accatattgc
     8461 cgagatggga tgcatcatat tccatgacgg ggcgttgcct gatgatactg accaccagtg
     8521 gtttgattaa catgttggtc gcggcccgtt gttgtatacc ggcggcgaaa atgatctcca
     8581 gagcattctg atcgataaca cgggccagat tcgcgttcag attttcaatc tcatgtccgg
     8641 tcagcaggaa actgccggca agccatgtca tctgctcctg aatcattgcc agaattttga
     8701 cgcgatcttc ttcaaccagc gctccggcaa tttttcctga tacagcggag gcaatcattc
     8761 cccccagaac accgccaacc acacgccctg tcagtgcacc aaatggtccc agtggactga
     8821 acaatatccc gcccgccact gacccgacag caccacctgc cacacaggaa gatgccacgg
     8881 caagattcct gatgaactgc gcaccggaga tccgtccccg caacattttg atcatgtcag
     8941 ggccggttgt gacagctacc agtgccagag atgtcaccag cgaacccttc atcactttgt
     9001 tcagggcact gatatttcct gcaccggtcc ccttctgaag cgcctgccgg acagtcgggc
     9061 tggccgtcga aaaatcaata tgcttcagca taccctgaac aacactgagc cggtgaagtt
     9121 gctgggttgt gacgtagaca gccagtgtgc gggtgaaggt ttttcctgcc tggacagcag
     9181 ctgtctgcag ggcagcatcg cgatcgccgg tgctgagcca gaagaccgat gccgtcaggg
     9241 caaaactgat ccctccggcc gccagactga caaccgaccc ctcggcaata tcataagtga
     9301 ccgattcgat ggtcccgaac cgggtgatat tacgggcctg ggtataagtc agatgtcccc
     9361 gacgaatcag ccgggacgct tctgcgggat cggttacacc gggtacttta ccttcgcgga
     9421 tcttattctt catggtttcc acagcgccgg catactgatc acgggggact tccagttgca
     9481 tgggaccatg atttcccatg taacgatact gtccgttctg tccgtcgaat gccgcactgt
     9541 taatgccagc gaatgtggcg ttgaatatcc gttgcgccgg tccgggtccc gatcatgatg
     9601 atcgggacca caaagaggcg atggttctgt cagaaggtca cattcagcgt ggcctgaccg
     9661 ttatagcctt cagcgctgtt gccgctgaca ctgtgggcat aaccggcctg aacgcccagg
     9721 gtgatatttt cccggacacg ggcttccagt ccggcctgca ggtccagtga cgtgccattc
     9781 cgggacggtg agaacgtcat gttactgccg gctgcggctg tacccatgct catgtctccc
     9841 cgggagctga aggtgcggat aacagaaggc tgtacccacc agttcacagg cagttcactc
     9901 acaccgtgtt ttgtactgtc gcgcagggtg tcacgggatg aggtgccttc accaaaggtc
     9961 atatcgttgt ggctgcccag acggaaaccg gcacgcacat gttgtgcact gccatgcccg
    10021 aacttcacat aaccggcgtt atcctggccg tcatccaggg aaagtccctg ccaggtatac
    10081 tgcagttgtg gctccagcat caggttgtca gtgatactga atggcagacc ggtttccagt
    10141 gagccctgcc agccccggcc ccgggcgcgg aagtcgttat tgtccgatga cgctttcatg
    10201 ctgtggcggg ttccctgtgc cacaatgtca gcccacaggc cggaggacgt gtgtaccaga
    10261 ttcaggtatc cgcccaggct gccggcatca tcccggaccg tgccggcacg ggagccgtca
    10321 tcatccttaa catcaacgga agaatggcct gcagcaccat acactcctgt cgtcagtgac
    10381 ataccggcaa cctctgttct gagcaggtca ccctccagac ggacgaagcc atagctgccg
    10441 ctgctttccg gcgtggcacc acggacaata ccgccgttgt tatcgtgacc gagatggccg
    10501 ccctgaatgc tgagacggac gctgttattt tcaccgttta caccggtctg atggctgcgg
    10561 gagcctgcca gaatccggtc atagtccatt gcctgtgtca gcatggaggc atacaggggg
    10621 acttcagcac gataagcatt ttcactgcgc agataccagc tctcatcact gtcccggttg
    10681 agggtgtagt taaaggcccc ggcctgcaac atattcccct ggacaaaagc cccttcctcc
    10741 gtggtggcac cgttaatggc ctcaaccacc tgaataccct taccggtggt cgccagcccc
    10801 gtaccactgt tgccggcgtt caccagattc aggatggttt ttccggttgc cctgccaccg
    10861 tcaatgacca gtctgtcagc attgttctgc gccatatccg ggcgtacacg caggctgatg
    10921 gtgccattct gcccgttcag gtttttcacc tgcagagttg tcggaacgaa cttccctgtg
    10981 cgggcggagg tgaaatgaat ctgtccggca tggctgaggt catccaccac cgactgaacc
    11041 gtggcgttat cggggatatt ccaggtggca ccggatgtca gggtgacatt cgtggggtca
    11101 atggcaccgt tcagcactgt gctgccggtc agcttcaggg ctgtaccgcg ctgagcaatg
    11161 acatccgtgg tgacggtact gtcgttcagc gtcagcgtgc cttcattcag gttgacggct
    11221 ttctgggtga gtgtggtgtt gctgacagtg agtgtgccac ttcctgattt ttccacgctg
    11281 ccgttaccgg taagagcgcc tccctgcagg agtgcatcgc cttcacggat ggtcagggta
    11341 tcgttgttca ccgtcttccc ggaaagggta agtgtggcgc cgttattcag cgtggtggtg
    11401 cccgccagtg tgccgcccct ggcggtgaac agtccgccat ttaccgtggt atccgtggcc
    11461 gtatcaccgg cgttcagcgt gaatgaactg cctttttcca gcatcagggc atccgcctga
    11521 ccgcctccga tactgaatgc ctttccgtcg ctccgggtac cactgacagc ggcaccggaa
    11581 tcggccagca gtacaccgcc attccccatg gatacggtgg tggcggcacc accgttgcgg
    11641 atatccagcg ttccgccatc atccacacgg gtattagtgg ctgtgtgtcc gctcagcaca
    11701 tccagacgtc cgccattttc cagtacgaca ttatcagcct tacccgccac aacggagaat
    11761 gctcccaggc ggtttgtgcc ggtgacggtt gcagctgtac tggtaaccag tgcaccgccc
    11821 gtgttctggg tgacatcaga cgctttaccg ccggcattca cctgcagctt tcctttctgg
    11881 ttgatggtgg tatgcgcggc agttcctcct tccttaatca cctgccagcc atcacggttt
    11941 atcagcgtct ctgttgccgt gccaccgttg tgtacatact ggttaccccc ctccagtcgg
    12001 gtgttatgtg cctctccgtg taccgtctgg tcaccaccgg cgtaaatgag ggtgtcccgt
    12061 gccatccccg aagaccagat aacctgccgg ccatttttat tgatggtggt ggattcagcc
    12121 gtccctccga ccatctgacc gctggacaca ttttcagact ccggaccacc ttctgcaccg
    12181 gtgttgacga tggttccggt tgccagtccg ccatgtttta tggtctggta accatcctgg
    12241 ttaataattg taccggcagc tttccctccc ccgtgtaccc actgctcacc tctgttatcc
    12301 agcgtggtgt tcaccgccag tccgttaagg ctctgccctc cgccatcacg aataaccgta
    12361 tcactggcag ttcctcctgc ctgcacaatc tgacgaccat ttgcggtgac agtggtgttt
    12421 ctgcctgtgc cacccgcttt tatccattgc ccgccggtgt tttcgtcact gtccggcccc
    12481 agctcaagcc cggtactgac agtcacgcca tcagctgttc cggatacaaa ctggttgtca
    12541 tggtttacca gtgttccgcc attcactgtt tcacacgggt gcacaacgat gtcagcagcc
    12601 agcaccggga gtgacgtgac cgcggcaaga gacagtgcca ccgccacacc gccacgttta
    12661 ccccgtgcgc gggccagttc ggaggcaacc acgaaagcgc ccgtaatgtg attccatacc
    12721 agcctgtagc aggtattcag atgtcgtttc atcagcattt ccttaatcag agtgagggtg
    12781 aataaaaaag ccggtatcca cagatgtgga taccggcagg taaagggtta cagtgccgga
    12841 ggtccggctg acggaagttt ctgacgtgtg gctctgttgt tccctgaatc tcatgtctgt
    12901 gtgtatctgc gcacgttgct aacaggagtt attattagca taacgatcga tattaacgat
    12961 cggtaaaatg ggtattgatc gttttattct tttatcaagc tattgcacgg acaagagaga
    13021 cagtaacgaa aacagcgctt ttgtgagcat acttagaggc agggaggaca tcatccacaa
    13081 caggttcaga agaagatcca gttccatacc gcgcgggcaa cagagaccac tgtgtcacgg
    13141 acggcacgca gaaccgtgcg tgcaacagag gcaacacaga cgctctccgc tgtgtcaaat
    13201 atccggtcca ccgcaccggt aaactgctca cgggcctgag cgcggacaga ttccgtgcgc
    13261 agctcgtcct gcaggcgggt catcagggga ctggcggcat ggtcgggaag cgctgtcatg
    13321 agcgcactga ccagcgtatc cacttcccag ccggtgcggg ccgatacggc cacaaccgga
    13381 tgtacaggcc ggaacagacg gaataccgcc tccgtttttt cgcgaatgtt ctgtgcctgt
    13441 gcgggagagg gctgaatgcc ggccatatcc cattcatggc agggctccgt tttgtcggcc
    13501 tgtgtcacca caaacagcac ctgctgatgt ccgcactgca ggatgtgtcg ccagaaatac
    13561 tcatccacag acagggcccg gtcatcggct ttaatcagcc acagcaccag gtccagttca
    13621 ggcagaatgt cacggtacag ggcttcatac tctgcatccc tgtcccggct ctcgcccacc
    13681 ccgggcaggt cagtgataac catgctgtaa ccgtgaccac tcagacggaa gcgctgcact
    13741 tcccgggtgc cggcgtgaac atcactgacc ggtgtgacct ccccctgaaa cagggcatta
    13801 cagagtgaag atttaccggc cccgctttta cccataatgc caatcacggg ctcgtgacgg
    13861 gtgagtttgc gcagatgttc caggatgtga cgggaaagcg agtaaggcag ggaggagagc
    13921 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
    13981 gacaaaaccc cgcacaccgg aaaggtggcg ggggatccac ggagataata tctgtctgaa
    14041 attgtttgga atcgatgggg atgttgcgta ttaacatgaa ggagggcagg agttatccgt
    14101 tattgggtga tatccttcca tgaaaatacg cggttattgt taaacagcag gctgattact
    14161 ccgtaaagga gaaaaatatt catcttgcag cctgattatc aggtgtgttt atgtttactg
    14221 aggtgagtat ttttcagtat attgtcgggg cagtcagtgg gagatattaa atttacgttt
    14281 agagttatct tgcttctgct gtgtattttt ataagagtgc gcacgtaatg atatctgttg
    14341 ataccatgtt gttaagtgtt tcccttccgg gtcatgcgta cttgcaaaat ggcatctgaa
    14401 tcgatcacgg tttggccaaa ccgtgatcga atagatagga atacgcacgt aatgattaaa
    14461 tgcttttgtt acaccattct tccggcatga atatttatcc agttcccttt tttatttgtg
    14521 tagtgatact gtcggaatag cgttcagtaa tgtgaagtgt cctgtaaaaa aactttaata
    14581 aatgtgtcat cctggtcagg aaatatctgc tgagatatta ttagttggtg gtgtttttac
    14641 tgagtaatag ctggctttta attcctgaac tgaaatactg tcgctcgtca gggtttaatt
    14701 aaaactggat gatgatgtga aaatgaaggc tggtttttgt gtttgtcgaa gaagtgttat
    14761 tttaatccat tctcatcatt ggctggcgat tatacagaag tatttgaaga aaaaatgttg
    14821 cattttatct gtgtcagcag ggaggaagta ttttattgat gtatcagtat taaatggttt
    14881 ttgttattta taaaaatgtc atcctgtcca tagaatattg tactcagcat tcagtgcagg
    14941 aactgtcatt ttctttattt tattcttctt caggagggca gttttctgaa agcgttactc
    15001 cggggaaact gtttatgttt attcccggca ggaaacccgt acctgcaata ccctggatga
    15061 tgctttttgc tggtacgttg ttgtgtgaaa tttaatcaac gtgtgtgttc cggagagcgc
    15121 ccgtagacgc acagattcat tctgacattc tttagcgaca aaataccgaa tggttattat
    15181 ttaatctgaa aatcgacaga aatttttatc ctgtattgat tctgctgcta tttttgctat
    15241 tagtagcaat agttgcggta ctttatgttc tgtgtgttgt gaggtcatta tgccatactt
    15301 tcagtatcct gatgagtttc ccctgagctc gctgcctcca ctcattcgtg atgccgttat
    15361 tgaagctcag caaatcacgc aggctcccct ggggcttgtg gcagcatctg ccctgggcgc
    15421 tgtttcactt gtctgccaga atctgattga tgtctgcaga ctgaatacac tacgggggcc
    15481 ggtttctctt ttcttactga cgcttgctga gtccggagaa cgaaaaaccg cagtggacaa
    15541 attattaatg gagcccctgt accagcagga gatgctgtta tattccagac acaaaaatga
    15601 actgacgaca tggaaaaata aggaagagct tttaaaggcg cagaaaaaag cattgttgtc
    15661 aaaactgaat aaagaactcc gcaaaggcgc ggatgagtca gaaacgctca gacaactgga
    15721 agcgcttcag aaaaacagag gggaaaaacc tgtaagatat aaattcattt ttaatgatgc
    15781 cacgactgcg gcaattaaga atcagctttg tggtaaatgg cgttcagtgg gtattatgtc
    15841 agatgaagcc ggtataatat ttgatgggta taccctgtct gagctgcctt ttattaacaa
    15901 aatgtgggat ggctccgtat tgtcggtgga cagaaagaat gaacccgaac agatgattga
    15961 aaatgccaga atgacattat ccctcatggt acaacccggt ctttttgacc gatatatgga
    16021 gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc cgttgtctaa tcagtaaacc
    16081 tgccaccacg caggggaaac gctttattaa cggagctgtg accccgggag gatcattaac
    16141 ggcatttcat gaacgattaa tggagctggc cagaggcagt attgaaaaat ccagtaagga
    16201 tgaacgttat tgtctgcatt tttctcctga agcgcaaaaa atatttattg atcactataa
    16261 tgttctggag caggatttgt ctccttccgg acccttaagc cagtttcggg gacatgtttc
    16321 aaaaaaaaca gaaaacattg cgagaattgc tgcgttattg cagtatttca gtcagggtga
    16381 agggaaaatc tctgccgata tcatgacgtc tgctgtcgta ataagttcgt ggtacacaga
    16441 tgaatataaa aaattatttg cgttacctga tgaatctgaa ctgcagctgc aggacgcccg
    16501 ggagctgctc gactggctca ttgaggagtg ccgcggagaa tgcccgcccc gtgtcaggaa
    16561 aaattatatt ctccagtgtg gcccgggccg gttccggaac aggaaaaagc tgaacgtcct
    16621 gctgaacatt cttgcatctc aactcaggct ctctgtcgtg caggaaggaa aaaccaccta
    16681 tgtcctgctt tccgaaataa acaatatcac gctgcctgaa ctgaatgcgt gctacagcca
    16741 gaaactgata agatggcatg agcaatcctt tggcttataa tgttccctgt tgctgagacg
    16801 gattattcag tggtggtatc gcagtatgcc cgtcaccccc gcttctgcat gagggtgacg
    16861 gaggaaaagt aagtgtgcgg aggcccggga gcgttatgaa tcaacaggcg tataacaggt
    16921 actcgtcaat catcagttcc agctcgcgct tttcccgttt gcgcgccaca gcccagtttt
    16981 caagctctgc atgtgattta tgctttaact cttcagtcca gggctgcatg gagtgggcac
    17041 acatcagatc ataccagcac agccatatca gcgtgtggta gagatcacga tcgtcgaaac
    17101 gccggagcag gctgttcagc ctgtttgcca gccggttttc acccagacga tgtagctcac
    17161 tgagtaaaac gccgcccaga ttaaaatgtg taagcagcat gatttcatgg gtctgaatgc
    17221 tggtcattaa cccttcctga atggcaggaa tgaacggtgg tgttcccggg ttgacgacct
    17281 gatagagtcc ctgtggtgcc actgagatga tggtgccttt ttctggcaga aaaaagtggg
    17341 ccagggtcag gcggtgataa ccatgaagat gcggagcccc cattccatga gcgacgataa
    17401 gcctcgtcac gtttgtcatc catttctgat gctggatgag cagcagctca caccggtttt
    17461 caagtttttg cataatattc tctcctttag cgcgcagcaa aatgcctccg ggggccggat
    17521 catgcctttg ctgcggttga acggataacg gtcaggaatt acggacggga ctgtgtaata
    17581 cgtgcctgca accaggcttc cacttcactt ttcagccatc gggagctacg gcccagtttg
    17641 atgggggcag gaaaggcacc atccttgatg agcttgtaaa accatttatc ggtcaggccg
    17701 gtcagctgag tgataaacga catgtcgacc atctggtcat ccatcagtga aactggggta
    17761 ttcatgaaaa tgttcctccg gatgttgtta atctcaaccg gcgttggtga tgaaaacaac
    17821 gcaccacctt ccccggcata acaaccgggg aaaaacgtaa aaaataatcg ggaatgtgac
    17881 cgtcaggaga taaacggggg gattgcggtg gctgagccgt accggtatgt cgggtgattg
    17941 ttgcgaaacg atgcttccgc acgcgcacgt gataaaggag ggaggacttt cgtgagacaa
    18001 ggggaaaact ggcgttctga taattgcaga gttgtttttg aaggcggttg tggtgcatca
    18061 gcccgtgacc tgtgggcagg cgacaggtat cactggtggg ataagactgt ctgattggta
    18121 ttataaatat ataagagagc tcaaacacat gagttcatat atgtgaaata aatctgtgag
    18181 tgtgatcagc agaccagtct acccagtagc aacagattgc gttgtactca cagggttatc
    18241 cctgataact ggcgtataac ctgcgtacat aaaacgtacc tgcaatgcac ctgaacagag
    18301 tgcattaagc atccgcctga gcggtaaacg tatccgatct ggtgagtgac agtctgagag
    18361 cattctcagt aatcgccccc ttgtatcctg gaaattggtt aagataacag cgattagatt
    18421 tggcaaggtg tatgcagcca gcagcttaac atctcagcta actggctggt taccttcagg
    18481 ttgtcgcaat ggcatcggac atccggtgtc ggataactaa acgtagcgtt aaggcgatat
    18541 tgctgagaga ccggttaagt ggttgcacgg gagtaatgag tttggggagg gtaccagaat
    18601 gtcagtctgg tgcggtatgc tggcagtatc acgttgatta cagcataaag taacgagaga
    18661 cgaatggaag ttaaaaccag agcagttccg tcgttgttca tcccgggaga cgctggaaaa
    18721 agtgatttcc cacacacgtt ataaacttac ccctgcggag ctggaagcct ttaactctgc
    18781 ggtcgat
//
LOCUS       DAPQZD010000086        18589 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPQZD010000086 DAPQZD010000000
VERSION     DAPQZD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.086"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..893
                     /locus_tag="RO454_002928"
     CDS             <1..893
                     /locus_tag="RO454_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDY0934729.1"
                     /translation="ALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIK
                     EVFISSGARVTAQDIPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSYENDQLTYTP
                     GADDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERP
                     VKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAALL
                     SSRDLSVYSMNAPGFIPGIDFSDHLNYWQYDIPAVMITDTAFYRNKQYHFPGDIADRL
                     NYQKMAQVVDGVTTLLYNSK"
     gene            996..1499
                     /locus_tag="RO454_002929"
     CDS             996..1499
                     /locus_tag="RO454_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDY0934730.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1566..3023)
                     /gene="nuoN"
                     /locus_tag="RO454_002930"
     CDS             complement(1566..3023)
                     /gene="nuoN"
                     /locus_tag="RO454_002930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDY0934731.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3030..4559)
                     /gene="nuoM"
                     /locus_tag="RO454_002931"
     CDS             complement(3030..4559)
                     /gene="nuoM"
                     /locus_tag="RO454_002931"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDY0934732.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4723..6564)
                     /gene="nuoL"
                     /locus_tag="RO454_002932"
     CDS             complement(4723..6564)
                     /gene="nuoL"
                     /locus_tag="RO454_002932"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDY0934733.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6561..6863)
                     /gene="nuoK"
                     /locus_tag="RO454_002933"
     CDS             complement(6561..6863)
                     /gene="nuoK"
                     /locus_tag="RO454_002933"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDY0934734.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6860..7414)
                     /gene="nuoJ"
                     /locus_tag="RO454_002934"
     CDS             complement(6860..7414)
                     /gene="nuoJ"
                     /locus_tag="RO454_002934"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDY0934735.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7426..7968)
                     /gene="nuoI"
                     /locus_tag="RO454_002935"
     CDS             complement(7426..7968)
                     /gene="nuoI"
                     /locus_tag="RO454_002935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDY0934736.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7983..8960)
                     /gene="nuoH"
                     /locus_tag="RO454_002936"
     CDS             complement(7983..8960)
                     /gene="nuoH"
                     /locus_tag="RO454_002936"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDY0934737.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8957..11683)
                     /gene="nuoG"
                     /locus_tag="RO454_002937"
     CDS             complement(8957..11683)
                     /gene="nuoG"
                     /locus_tag="RO454_002937"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDY0934738.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11736..13073)
                     /gene="nuoF"
                     /locus_tag="RO454_002938"
     CDS             complement(11736..13073)
                     /gene="nuoF"
                     /locus_tag="RO454_002938"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDY0934739.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13070..13570)
                     /gene="nuoE"
                     /locus_tag="RO454_002939"
     CDS             complement(13070..13570)
                     /gene="nuoE"
                     /locus_tag="RO454_002939"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDY0934740.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13573..15375)
                     /gene="nuoC"
                     /locus_tag="RO454_002940"
     CDS             complement(13573..15375)
                     /gene="nuoC"
                     /locus_tag="RO454_002940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDY0934741.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15469..16131)
                     /gene="nuoB"
                     /locus_tag="RO454_002941"
     CDS             complement(15469..16131)
                     /gene="nuoB"
                     /locus_tag="RO454_002941"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDY0934742.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16147..16590)
                     /gene="nuoA"
                     /locus_tag="RO454_002942"
     CDS             complement(16147..16590)
                     /gene="nuoA"
                     /locus_tag="RO454_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDY0934743.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(17220..18158)
                     /gene="lrhA"
                     /locus_tag="RO454_002943"
     CDS             complement(17220..18158)
                     /gene="lrhA"
                     /locus_tag="RO454_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDY0934744.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4661 a         5145 c         4797 g         3986 t
ORIGIN      
        1 atgcattacc accaacgccc cgacacgcta gtcctgagca attagaaaaa acagttcgtt
       61 atcttacaca aactgtgcat ccacgtagcg ccgacaatat tgataatctg aatagatccg
      121 cagagtatat aaaagaggtc tttatcagta gcggtgccag agttaccgcg caggatatcc
      181 ccattacggg tggcccctac aaaaacattg ttgctaatta tggtcctgcc gatggaccgc
      241 tgattattat tggtgcgcac tatgactctg tcagtagtta cgaaaacgat caattgacct
      301 atacgccggg cgcggatgat aacgccagcg gtgtggcagg attactcgaa ctggcacgtt
      361 tgttacagca gcaagcaccg aaaacaggcg tgcagctggt cgcctatgcg tcggaagaac
      421 cgcccttctt tcgtagcgat gaaatgggga gcgcggtaca tgcagcttcg cttgagcgtc
      481 cagtgaaatt aatgatagcg ctggagatga ttggttatta cgactctacg cctggtagcc
      541 aggattaccc atccccggcc atgtcctggc tttatcccga tcggggagac tttattgccg
      601 tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca ttgttgtcat
      661 cccgggattt atctgtttat tctatgaatg ccccaggttt tattcccggt attgatttct
      721 ctgatcacct taattattgg caatacgata ttcccgccgt aatgattact gacaccgctt
      781 tttatcgtaa taaacaatac cactttcccg gtgatatcgc agacagatta aattatcaga
      841 aaatggctca ggtagtggat ggagttacaa ccttgttata caacagtaaa taataaaaat
      901 ttctcgaggc ggatccagaa aaagcaacat gcgcatttta ttttctttcg tctgttttaa
      961 gatgcgaaac atgaaattca acggaggaaa aataaatggg aatgattggc tattttgcag
     1021 aaattgactc agaaaagatt aaccaactgc ttgagtctac taaaaaacct ttgatggata
     1081 atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt
     1141 tacactttgg cctgacgggt acatctgcat ttgaccccgc gaagaatgac ccgctaagtc
     1201 gcgcagtgct tggtgaacac agcctagaag atggcataga tggcttcctg ggattgacct
     1261 ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc
     1321 gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga gtaacctttt
     1381 cagaagagtt agaggggcaa ctctttgctt cgatcatgct tgatatggaa aagctgatta
     1441 gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat
     1501 ggtgtcaggg cacccaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaactg
     1561 actgcttaca tcagcggcat tgctaaccgc acaatgctaa tcagcggttg tggccataca
     1621 cccagcacca gtaccaacag tgcagagatc agcaccacga taccgcctgc gctgtactgc
     1681 cagtttgatg gtgcatcgcg acccggttgt tccggagcgt gaaggtataa gctcaccgcc
     1741 acacgcaggt agtagtagag gccgattgcc gaaccgacaa ctacggcacc caccagccac
     1801 cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc
     1861 attgggatac cagccagtga aagcatcatc accgtcatca ctgccgcgag gatcggacga
     1921 tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg
     1981 gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc tgccaggtaa
     2041 acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga
     2101 tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatattggt ctggctcagc
     2161 gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagcac tacgcgaatc
     2221 gcttcactgt cacccaccgg tgcgtaaagg aacaaacgca tcaccacgcc gaagatagcg
     2281 attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct
     2341 ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcagg
     2401 ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagt
     2461 gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa
     2521 gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg
     2581 cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcgat accgaggaac
     2641 agagacgcca gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc
     2701 aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta
     2761 caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg
     2821 cgcatcagtg gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc
     2881 gccgcgttaa gcccaataac cgagagcgta gcgttgagaa aatgattgcg tcgccacgca
     2941 atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc
     3001 aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca
     3061 aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag
     3121 aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac
     3181 atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaaata agcgcgatgc
     3241 agcatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatt
     3301 accgggacaa cctggaagct accgaacaga atcataaatt cgccgacgaa gttaccggtg
     3361 ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccacttcatc
     3421 ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc atagagctga
     3481 ccgcagagaa taaacagacc cgccgctgac aagccgtgtg caatcatctg gattaccgcg
     3541 ccctggtagg ccaactggct accggtgtag atagcaatca gcacgaagcc catgtgggaa
     3601 accgaggtgt aggcgatcag acgtttgata tcggtctggg cgaaggccat ccacgcacca
     3661 tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc
     3721 gggaacagcg gtagggagaa acgcagcaga ccgtaagcgg cggttttcag caagatcccc
     3781 gcgaggtcaa cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc
     3841 agcggaacca ccggcatttt cactgcgaag gcgatgaaga agcccagcat caacaggtat
     3901 tccacgccgt tagacatcgg cgtattcagc agctcttcat agttgaaggt ccagacgccg
     3961 gtcgcattgt agtgaacgaa aaccagcgcc tggatggcga tcaacatcac cagaccactc
     4021 gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct
     4081 ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag
     4141 aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc
     4201 aggttgaggt ggaagaagcc ctggtatttt tcgatctctt tccacgaaca gagtaccgcc
     4261 agcacaccga gcagaccggt cagcacgacc atcagcaacg acagaccgtc aatggcgaga
     4321 tggatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga
     4381 attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc
     4441 agcgtcaatc ccatggttac cagcgcgatc cagcgcggca ccttgacgcc aaagcgttcg
     4501 gtctgccagc acaggaagcc gccgataaag ggaattaata ttagccaggg tagtaacatg
     4561 gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac
     4621 agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaagacgg cttatccgac
     4681 ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa
     4741 cagagccagc accacgaccg caccgatgct catggatgcc acataccagc gcagataacc
     4801 gttctcgctt aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat
     4861 cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa
     4921 cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtac ccagcagacg
     4981 gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc
     5041 agccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct
     5101 gccgtgcgcc agctccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc
     5161 accaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt
     5221 cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt
     5281 gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga tatgtccatt
     5341 cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag
     5401 tgccgatagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg
     5461 cagaccaccc atcttaaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga
     5521 tgccaggaac agcagcgctt taaagaacgc gtgggtcatc aagtggaaaa tcgccgcatc
     5581 ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc
     5641 gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac
     5701 cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt
     5761 acgggcgatc aggtagacac ccgcggttac cattgttgcg gcgtggatca gcgcggagac
     5821 aggcgtcggg cccgccatcg cgtcggcaag ccatgtctgc aacggcaact gcgcagattt
     5881 accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc
     5941 aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta
     6001 aagaatgaac agcgcaaaag cgaggaagac gtcacccaca cgggtcacga cgaacgcttt
     6061 cattgccgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca
     6121 caggcccacg ccttcccagc cgaggtacat caacagtagg ttatcggcga gaaccagaac
     6181 taccatgctg gcgatgaaca ggttggtgta agcgaagaag cgagagtagc cctcttctcc
     6241 gcgcatatac caggaggcat acatgtgaat aaggaaaccc acaccagtga ccactgagag
     6301 catggtcagc gacaggccgt ccagcaccag attgaaaccg atgttaaagt cgcctaccga
     6361 catccacgtc cacagcggct ggctatatgc ctgctcaccg ttagcgaaga agtcaacgcc
     6421 gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgagac
     6481 gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat
     6541 aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc
     6601 tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc cgccgccgcg
     6661 aggctgatgg cgagaatgta catcacctga ccgtcggtct ggccccagta gcttccggcg
     6721 accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc
     6781 agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc
     6841 agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt
     6901 tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca
     6961 gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg
     7021 taatacccac tgctttagca ctgattggcg taccgtcgat accttgatcg ttaacaccga
     7081 gaatggcgta aacaatcacc accagcatga tggctgacaa aattgccgga ccaatccaca
     7141 cctgcggttt caaccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca
     7201 tcaccacgaa cacgaacagc accataatgg cacccgcata gacgataatt tccagcgcac
     7261 cggcaaagta agcgcccagt gagaagaaca ccccagagat cgccagcagc gaaataatca
     7321 ggtacagcag cgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg
     7381 ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct
     7441 cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc
     7501 catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag
     7561 atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt
     7621 taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga
     7681 gaagttgatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag
     7741 cgagatacag ccgaccgggc aggctaccgc gcagaggtta caggctacgc aacgctcttc
     7801 gccgtccgga tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg
     7861 ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat
     7921 actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc
     7981 ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc
     8041 ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac
     8101 gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat
     8161 ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcag tgcagagatg
     8221 gtcacaatcc cgatgtattc gcccacgaag aacagaccga acttcatacc ggaatattca
     8281 atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga
     8341 cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc
     8401 cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc
     8461 acgcccatca aggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc
     8521 atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa
     8581 accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc
     8641 caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc
     8701 ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac
     8761 attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca
     8821 taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag
     8881 gtgactacca ccagcaggat caccaccgct ttaaggacgg tcagcaggat ctcaatcagt
     8941 tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca
     9001 gcaccggagc aatgccggac atacccatcg gtaagcccac ctgccctgcc gtcagccctt
     9061 cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac
     9121 ctgcgttcac ccccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca
     9181 tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt
     9241 acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt
     9301 aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aaacgcagtt
     9361 tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cctggtgccc
     9421 aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg
     9481 tgaacatagt gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca
     9541 tggcggtacg accgctatag cgatgcggtt cacgggccag tttctgccca cgaatacgga
     9601 atgtcgcatc cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag
     9661 cgtcaatcac gtggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctatgca
     9721 gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat
     9781 cgtaaacctg gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt
     9841 cagcaaagct ggcggcggaa agtaccagat gggcgttttc cataatcgct gtgcgttgat
     9901 ggtcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcggaag
     9961 cgtgacgatg cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt
    10021 cggttaacgc ttcttcaagc gatccaccgc ccataatgcc cagccccatg ctgttgacgg
    10081 aacgggcaat catggtgata ccgacgtcag cgccgcgacc tttcagggct ttcgccacgt
    10141 tagccgcagc ctgaatcact tcagcgctac cagcgttcgt cccggagata atcaacggtt
    10201 tcttcgcacc agccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggctcaa
    10261 taccgtcaac cgctggtgca gagttatcca gcgcatgagc gatggcaaaa cctaaacgcg
    10321 cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat
    10381 caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa
    10441 tctgccagtc agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcaccgcct
    10501 gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta
    10561 ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagta
    10621 ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggta tagaagtttt
    10681 cttcgcccac cagctcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa
    10741 taccgatcac tttcttcgac tgacgcagaa tatctgctgc gccctgcatt gcctgttcgg
    10801 cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatctttca
    10861 ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac
    10921 cgttgtaacg gttttcgata cgacgcagtt cgccataacg ctcgccgggg ctgatgttac
    10981 agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt
    11041 aacgctcgga gtgcgttttg tcggtgaata cgccggtcgg acaaatttcg accaggttac
    11101 cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg
    11161 caccgtaaac gcccaggtcc gtaccgtcag cgtaatcttt gtagtaacgc acgcagcggt
    11221 agcaggcgat gcagcggttc atttcgtgag agatgaacgg ccccaaatcc tgattacggt
    11281 gagtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct
    11341 gaaggtggca gttaccgccc tcttcacata ccggacagtc gtgcgggtgg ttggtcatca
    11401 accactcgac cacgctttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg
    11461 tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt
    11521 tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc
    11581 agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgccc
    11641 cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt
    11701 tctcagtcag agactgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc
    11761 ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat
    11821 tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca
    11881 cagaaagttt tacccggacc taagaatcga cacagttgct caagtgtttc gatatcgccc
    11941 ggctgacctt caccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg
    12001 cggcatggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc
    12061 agcgacacca tgttaatctc gtggtcaacc gccatcgcca gcgccgtacc cagacggctt
    12121 cccgctttac cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc ggtcaggaag
    12181 tcggtgcccg cgccgcctgg ctgccaggct ttgaatttca ggccgtcacg cataccacca
    12241 gcgtaatctt caaggatttc acgcgcggtg gtgccgaacg gcagttccca cagacccgga
    12301 tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg
    12361 ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg
    12421 ttgttaacgc aggttggttt accccatgca ccggaggttg ccgggaaggg cggcttcgag
    12481 cgtgggttag cgcgacgccc ttccagggag ttgattaacg ctgtttcttc accgcagatg
    12541 tagcgccctg ccccggtatg gacgaacagt tcgaaatcga aacctgttcc cataatgttt
    12601 ttgccaagca gacccgcttc ggtggcttcg gcaatggcac ggcgcagatt cactgccgct
    12661 tcaatatatt cgccacgcaa gaagatgtag ccacgatacg ctttcagcgc aaacgcagaa
    12721 atgagcatac cttccaccag caggtgcggt agttgctcca tcagcagacg gtctttatag
    12781 gtgcccggct ccatttcatc ggcattacac agcaggtaac ggatgttcat ggattcgtct
    12841 ttcggcatca ggctccactt caggccagta gaaaagcccg cgccgccgcg ccctttcaga
    12901 ccagagtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc
    12961 gcgccttcgt aaccgttttt gctgcgatat tcgtccagcc acactggctg tttgtcatcg
    13021 cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc
    13081 gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca
    13141 tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcgac
    13201 cgtcaaatgt cgtttgccct ggtttgatgt tcagtttttt ctccagcgcc gcctgaatac
    13261 cctggtaacc gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcggccta
    13321 ctggctgacg gaagatctgg ctataaaacg ttgccacgcc ttcgacgtcg ctcgccggaa
    13381 tacccagcac atcggcgatc gcgtggatcg cgccatccgg cacccagcca cgctgcttct
    13441 gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt
    13501 gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat
    13561 tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac
    13621 aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata
    13681 actcggcgta cggatacggg tgcgataact catggtgctg ccgtcgctgg tcaggtagta
    13741 actgttgatc cccttggtcg cctcaaccat ctggaaggat tcattggcag gcatcaccgg
    13801 accccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc
    13861 tttcggcggc ggcgtggtca gcggatgatc cgccttgaac gggccttccg gcatgttgtt
    13921 gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg
    13981 ggtgtagcag tcagaaacac caccacccac cgggatttca aagtcgaagt tttcatagcc
    14041 agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc
    14101 agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc
    14161 tttcagaatg gtgttttgca gcgccgcttt ctcgtaagac gccagacgtt taggcatcca
    14221 gtcgaggaac tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc
    14281 aatacggaac cacgccgggt gcatacgaaa accagtgatt gcttccacca gatcgtaaat
    14341 tttctgacga tcggtaaagg cgaagaacac tggcgtcatc gcgccgacgt cctgaataaa
    14401 ggtggagata tacagcaggt gactgttgat gcggaacagt tcggagagca ttacgcgaat
    14461 gacgttaacg cgatccggca cggtgatccc agccagtttc tctaccgcca gcacataagg
    14521 catttcgtta acgcagccgc cgaggtattc gatacggtcg gtatacggaa tgtagctgtg
    14581 ccaggactgg cgttcgccca ttttctcagc gccacggtgg tggtaaccga tgtctggtac
    14641 gcagtcgaca atctcttcgc catcaagctg caaaacgata cggaaagcac cgtgcgcaga
    14701 cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat
    14761 cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt
    14821 cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc
    14881 tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga atgtaatgcc
    14941 aaatagatcc caggtttcac gctcatacca gttagcgttc gggaatagtt tggtaaaggt
    15001 cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc
    15061 gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt
    15121 gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt
    15181 ctttaagaaa tcgcccactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc
    15241 ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat
    15301 caccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt
    15361 catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa
    15421 tagatttagg aataagcgaa gaaaattccc tttgccgaca ggcgcaaatt aaatctcgtc
    15481 aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg
    15541 catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt
    15601 accgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcggaca
    15661 gcccgggata tacacatcaa ccgggatgaa tttatcaacg ccctgcacaa cggaataaat
    15721 atcgtacata ccgccggagt tggcacaggc acccattgag ataacccatt ttggttccag
    15781 catctggtca tacaaacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac
    15841 caccatcagg tcagcctgac gcggcgaagc acgcaatact tcagcgccaa aacgcgccac
    15901 gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa ggccgaagtt
    15961 atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa
    16021 cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg
    16081 gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc
    16141 tcgcggttag cgttgacgat tagcgatact gtccgtttcc gggttcatac gctcgcggcg
    16201 tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag
    16261 taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga
    16321 ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac
    16381 cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc
    16441 aaacggaacg ttttttgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag
    16501 catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc
    16561 gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc
    16621 actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt
    16681 acaataactg cagaaactat tttatacagc taattgatgt ggtcttttac tcctttctat
    16741 aaccttttgt caactttaac aaaggtttct tcacactgat ttacacaatg acaacaacat
    16801 ttgcatttgg cgccctttct ccccagatca ttgacgactc caggaaaatt agatgttgtt
    16861 gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg
    16921 tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcta
    16981 taacaaaaac gcagcaacaa attcacgtat tttttaacat tattgtagca ggagattttt
    17041 ttcaggtgat tatttgtgcg tacggggcgt gaatctctgg tgattgaaaa atgaacagtt
    17101 ttatacgttc tgcacgatac gaggaaggct ttttactaaa aaaaagccgc tggggtttaa
    17161 aataaaccca gcggttcgtt tttacactat tgtctcagga attatctatc gtccgtcgat
    17221 tactcgatat ccctttcaat caacaatgag tcatctcctt ccggagcaga cattgggctg
    17281 tattgccacg gattatggta gctctccatc gtctgataga tcacctgggc cagctcatta
    17341 ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg taagccatct
    17401 actccactca gcacacgcag gtccggactc atcatctcga ccggccttgc cgtcacgcca
    17461 agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc
    17521 cagggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca
    17581 tccagcaaca caagagggat cggctcacct ttttgcaata cgtattccgc tgcgcagtac
    17641 cagtgtgttg gcgaggtacg caggttcaat gccttaaagg tagaaggacg atgggtggtt
    17701 accatcaaat ccacttcctg ggattccagc atttctgcca tgtaggcatt acgtttaacg
    17761 cggacatcca gcgctagttt cggataaacc gaactcacgc gatttaacag gaaaggtaag
    17821 atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac
    17881 attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt
    17941 tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca
    18001 acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg
    18061 gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg
    18121 aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt actggcggct
    18181 catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct
    18241 ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa
    18301 aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg
    18361 atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa
    18421 atgtaaatgt gagtccttgt tccactctcg tacagcatcg ctggtcatac gcgaacacgt
    18481 acaaacagca atggtgaggc ggcatcaaga gcagggatcc gtcatttatg caagcatttt
    18541 accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgca
//
LOCUS       DAPQZD010000087        18575 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPQZD010000087 DAPQZD010000000
VERSION     DAPQZD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.087"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(13..834)
                     /gene="dapB"
                     /locus_tag="RO454_002944"
     CDS             complement(13..834)
                     /gene="dapB"
                     /locus_tag="RO454_002944"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDY0934745.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(995..1360)
                     /locus_tag="RO454_002945"
     CDS             complement(995..1360)
                     /locus_tag="RO454_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDY0934746.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1383..2297)
                     /gene="rihC"
                     /locus_tag="RO454_002946"
     CDS             complement(1383..2297)
                     /gene="rihC"
                     /locus_tag="RO454_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDY0934747.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2363..3313)
                     /gene="ispH"
                     /locus_tag="RO454_002947"
                     /gene_synonym="lytB"
     CDS             complement(2363..3313)
                     /gene="ispH"
                     /locus_tag="RO454_002947"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDY0934748.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3315..3764)
                     /gene="fkpB"
                     /locus_tag="RO454_002948"
     CDS             complement(3315..3764)
                     /gene="fkpB"
                     /locus_tag="RO454_002948"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDY0934749.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3872..4366)
                     /gene="lspA"
                     /locus_tag="RO454_002949"
     CDS             complement(3872..4366)
                     /gene="lspA"
                     /locus_tag="RO454_002949"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDY0934750.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4366..7182)
                     /gene="ileS"
                     /locus_tag="RO454_002950"
     CDS             complement(4366..7182)
                     /gene="ileS"
                     /locus_tag="RO454_002950"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDY0934751.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7225..8166)
                     /gene="ribF"
                     /locus_tag="RO454_002951"
     CDS             complement(7225..8166)
                     /gene="ribF"
                     /locus_tag="RO454_002951"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDY0934752.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8174..8392)
                     /locus_tag="RO454_002952"
     CDS             complement(8174..8392)
                     /locus_tag="RO454_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDY0934753.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8495..8758
                     /gene="rpsT"
                     /locus_tag="RO454_002953"
     CDS             8495..8758
                     /gene="rpsT"
                     /locus_tag="RO454_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDY0934754.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            9147..10520
                     /locus_tag="RO454_002954"
     CDS             9147..10520
                     /locus_tag="RO454_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934755.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
     gene            10548..12587
                     /locus_tag="RO454_002955"
     CDS             10548..12587
                     /locus_tag="RO454_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDY0934756.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(12645..13544)
                     /gene="nhaR"
                     /locus_tag="RO454_002956"
     CDS             complement(12645..13544)
                     /gene="nhaR"
                     /locus_tag="RO454_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDY0934757.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(13610..14776)
                     /gene="nhaA"
                     /locus_tag="RO454_002957"
     CDS             complement(13610..14776)
                     /gene="nhaA"
                     /locus_tag="RO454_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDY0934758.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(15011..16270)
                     /locus_tag="RO454_002958"
     CDS             complement(15011..16270)
                     /locus_tag="RO454_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDY0934759.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            complement(16399..17892)
                     /locus_tag="RO454_002959"
     CDS             complement(16399..17892)
                     /locus_tag="RO454_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDY0934760.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            complement(17913..18506)
                     /locus_tag="RO454_002960"
     CDS             complement(17913..18506)
                     /locus_tag="RO454_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934761.1"
                     /translation="MTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFD
                     SDMTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYG
                     QTNGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIA
                     WKFYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
BASE COUNT         4801 a         4838 c         4319 g         4617 t
ORIGIN      
        1 aatattttgt ggttacagat tattgagatc aagcacatct cgcatatcaa aaagaccact
       61 ttccttacca ctcaaccaca aagccgatct taccgcaccg ttagcaaatg tcatgcggct
      121 ggatgcctta tgggtgatct ccagacgctc gccaatatcg gcaaacatcg cggtatgttc
      181 gccaacgatg tcacctgcac gcacggtggc aaaaccaatg gtgccaggca cacgttcacc
      241 ggtatggcct tcacgactgt agaccgcgca atctttcaga tctttatcaa gagcgtgggc
      301 gatcgcctct cccattgcca gtgcggtgcc tgacggcgca tcaactttat gtctatgatg
      361 tgcttcaata atttcgatat cggtgtagtc ccccatcact ttggctgctt tctccagcag
      421 tttaagcatg acgttaacgc caacgctaaa attggcggca aagacaatcg caatatcggc
      481 ggcggcgtca cgaatcgcct gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat
      541 ccctttgcca tgctggcgac aaaaagcgag atggttcagc gtaccttccg gacgggtaaa
      601 atcgataaac acatcaaaat catcttttat cgcatcaaga ctactttgca cagtaacgcc
      661 tgttttcccg gctccggcca gttcgcccgc atcgctgccc agtaaagaag atccttcgcg
      721 ctccagcgcc gcgcccagct gcacgccttc taatgccagc gccgcttgaa tcaactggcg
      781 gcccatacgg cccccggctc ccgcgatggc aacgcggatg tttgcatcat gcatagctat
      841 tctcttttgt taatttgcat agaccgtttt cagagtaacc agcgcatcgc tacgccgcca
      901 gccaaaaacg ctgataatta gaaattaatg ataaacaggg agggatatca gtgaaaggca
      961 tgacgcagaa agtgatgcgt cataccctct ggcattatgc ctggtcttgt gactttgacg
     1021 gcaggcagca cagtataagc agaattacgc ccgtaacagg gatcaacacg acaatggtaa
     1081 cccaccaccc tgaatatccg atatcgtgca tccttctgac ggctaacgct atcgccggta
     1141 cggcaaaaat caacgttaaa ataaatgacg cgggcagcgc aaaaaaaatg aaaacaccat
     1201 agtcattgtt aattgttacc gctaaccacc aacataaaag cggaagcaga cagataacgc
     1261 cactaccaat cataaacgac cagaactcga agcgattgga aacaccttca taattaaaga
     1321 tgtttttcca cccttccaga tacgcttctc catacgtcat agaacacttc cttgttttat
     1381 tattagagcg ccagggccag cacctcagcc acccactgct ggaagccttt cacatccaga
     1441 tccagcgcca cctgtacatt ggctggtttg cccaggcaac cgtcgatatc aacaaccgtc
     1501 gtgcctgagg taaattcacc ctgagtttct accgcgacaa aacacggttt gagtgtgaac
     1561 aattccgggc gcaccagcca ggcgatagcg caaagatcgt gcatacgtag tccactttgc
     1621 atactgccgc tacggtagtg gctaaacagg gcgtgtagca tttttccggt gcgattcaac
     1681 tccggcaaag tagcgagata gtcaggggtt aatattgcct gattggtgac atccagaccg
     1741 cacatgacga tttcgatgcc gctacggaag acacaggctg ccgcttctgg atcggcagcg
     1801 atattaaact cggcgtttgg tgtgcagttg ccgcgaccag cagaaccgcc catgatcacc
     1861 agacggcgaa tatacggctt acattccggg cattgtgaaa gtaacagcgc aatattggtt
     1921 aacggaccga tagccaccag ggtaacgggc tctggcgcac gcatcagggc atcacgaatt
     1981 gccagaaacg ccggtatacc cagtggctga cggttgtgtt caacaaagtt atagccagcc
     2041 attcccgatt caccgtgtac agatgccgca tcgcgcggcg cacgaaccag tggtacggca
     2101 gcgccctggg caagcggaat atccacattc cagaaatgca gcagttgcag ggcgttgcgg
     2161 gtggttttct ctaccgaaac attacccgcg actgtggtca tcagttgcag atcgagttcg
     2221 ggggtaaaaa tcgcggcggc aatggcaacg gcatcgtcaa tgccggggtc tgtatcgagg
     2281 aagataggta aatgcatgtt ttctccataa aaaatgccgg taacaagacc ggcattttcg
     2341 cataacttag actactaatg acttaatcga cttcacgaat atcgacacgc agctctttcg
     2401 gcacttcgaa gacgatattt tcttcgcggc cttccagcgg aatggcttcg ccaccgccaa
     2461 gctgctgcaa gcgtgccacc acattctgca ccagaatatc cggagccgat gcgcccgcag
     2521 tcacgccgac gcatttaacc tctttcaccc actcttcctg gatatctttc gcatcgtcaa
     2581 tcaaaaatgc gcgtttgccc atacgctggg ccagttccgc cagacggttg gagttggagg
     2641 agtttttcga accaaccacc agtactactt ccgcctgctc tgccagggcg cgtaccgctt
     2701 cctgacggtt agtcgtggcg tagcagatgt catctttgcg cggaccgaca attttcggga
     2761 agcgtttacg cagcgcgtcg atcacatcag acgtgtcatc caccgacagc gtggtttggg
     2821 tcataaagga gagcttctct tcatttttga ccgtcagttt ccacacatcg tctggcgatt
     2881 cgaccagata cattccccct tccgggttgc tgtactggcc catcgtccct tccacttccg
     2941 ggtgcccggc gtgaccgatg agaatagatt cttcaccacg acggctggcg cgggcgactt
     3001 ccatatgcac tttggtcacc agcggacagg tggcatcaaa caccgtcaaa tcgcggcttt
     3061 tcgcttcgtt acgtactgcc tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt
     3121 ccggtacttc gctaatctgc tcaataaaga tagccccacg ctcgcgcagg ctatcgacca
     3181 cgtagcggtt atgcaccact tcgtgacgga catatatcgg tgcgccgtaa atggccagcg
     3241 cgttttcaac aatgctgata gcgcggtcta ccccggcaca aaaaccacgc gggttggcca
     3301 acaggatctg catgttacgc ctccagtgcc ggatcgattt ccagcacttc aatatcaaaa
     3361 tgaacggtct gcccggccag cggatggttg aaatcaacgg taatggagtc gccgttaatt
     3421 tcgcggatca cgccaggcat ctcactgcca tccattgcgg taaaaagcat gattgcgcca
     3481 atttctggct cgcctgcatc cataaattca cggcgggaga agtactgaat caggtccggt
     3541 gacggcacgc caaacgccgc atctggctcc agcgagaagg tggttttatc gcccactttc
     3601 agccccagca ggtgttgctc cagcccttca gaaagagaag catcacccag gcggaacagc
     3661 gccggtttac cgttgttgcg ggtagactca gcggtggtgc catcatcgag tttcagcgtg
     3721 aagtgcacca ggacggcgct attgctctgt acagattcag acatgcaggt ttgctcttat
     3781 attttgagga tttttgccgg atgcggccta cgatttcgca gcaatctgta ggccggataa
     3841 gatgcgtcag catcgcatcc ggcagggttt attattgttt tttcgcttta gaaggtaaaa
     3901 aaccttccag cacaatcagt gccgcaccga cacaaatggc agtatcggca aggttgaagg
     3961 tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag ccgtgccaca
     4021 ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg atattgttta
     4081 gcttctgcgc ggccttcgag cgatacatca tcactaccag gatcacgcta ataccaatcg
     4141 caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg
     4201 cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc gtatccccca
     4261 gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc acgactacca
     4321 ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg caaacttacg
     4381 tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg cgtgttccgc
     4441 caccttgccg acatcctggg tgtagtgcca gcagcgaggg cacttctcac cttcggcttt
     4501 actcaacgcg actttcagcc ctttgaggac ttcgctctgc tgggcatcag caggtgcgtc
     4561 gttatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt catcgcccag
     4621 cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgcag cttccagcga
     4681 gccgcccact ttcttatcgg cacgcgcttg ctcaatgact ttgttcactt cgccacgcac
     4741 tttcaacagc tcgtcccaga acgcatcgtt cattgcttca ctgtctgcca gaccaaacag
     4801 gccttcgtac cactcgccgg tgaagacgta tttttcacgt tcgcccggca ggtagcccca
     4861 cacttcatca gcggtgaagg agaggattgg tgccatccag cgaaccagcg cttctgcgat
     4921 gtgatacagc gcagtctggc agctacgacg cgccacgctg tccgctttgg cggtatactg
     4981 acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga agcgcatcag
     5041 acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc
     5101 cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg gtttcaccat
     5161 atcttttgcc ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg tgttacggat
     5221 acgacgatag ctgtcggcag cacgtttcag aatctcgtca gaaacagcca tttcgccggt
     5281 gtagtcggtt gatgccaccc acagacgcag aatatccgca cccagtttgt tcatcacatc
     5341 ctgcggcgaa acggtgttac cgatggattt agacatcttg cgaccctgac catccacggt
     5401 aaagccgtga gtcagtacct gacgatatgg cgctttgccc ttcatcgcgg tagagatcat
     5461 cagggaagac atgaaccagc cacggtgttg gtcagaacct tccagataca tgtccgctgc
     5521 gtgaccggca aattccggac gcacatcaac aacggaagag tgggtagatc cggagtcaaa
     5581 ccatacatcc agcgtatccg gtactttcac atactggtca gcttcgtcgc cgaggatctc
     5641 tttcgcatcg agatcccacc acgcctggat gccgtcaact tcaacgcgtt ttgccacttc
     5701 ttccatcagt tcgagagtac gcggatgcag ttcttctgtg tctttgtgca cgaacagtga
     5761 catcggcacg ccccaggtac gctgacgaga gatacaccag tcaggacggt tagcaaccat
     5821 cgactcgata cgcgcctggc cccagtcagg gatccactgc acgcctttga tctctttcag
     5881 tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg tcgcgcggaa
     5941 gatgatcggc gttttatgac gccagcagca cggatagctg tgttgcattt tctcaacgtg
     6001 caacagtgcg cctttttcct gcaacaacgc aatgacaata tcgttcgctt tgaagacgtt
     6061 aacgccatcc agagtcgggt aagtacccgg cagataagtg ccgtccgggc caaccgggtt
     6121 agcggtttcc agaccatatt tttgaccgat cacatagtcg tccggaccgt ggcctggcgc
     6181 ggtatgaacg gcaccggtac cggcatccag cgtaacgtgg tcgccaagaa ttgccggaac
     6241 atcgaagtcc ataaacggat gggtaaagcg caacagttcc agctcagcac ctttcaccgt
     6301 gccaagaatg gtgtaatcgc taacgccgat acgctgcatt acgctttcaa ccagatcttt
     6361 cgccagaatc acggcctgac cgtcgatttg caccagcgca taatcaaaat caggtgcaat
     6421 ggagattgcg cggttagcag gcagcgtcca cggcgtggtg gtccatataa ccagcgaaat
     6481 tgggccgtta acattgctta cgccaaattt cgttttcagc gcatcctgat cgaccgcctg
     6541 gaaagcgacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg cttctgccag
     6601 tgcagaacgg cagtcaacgc accagtgcac cggcttcgcg cctttgtgca ggtgaccgtt
     6661 gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt ccatggtcag
     6721 gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc gctgaccgtc
     6781 aacctgggtc gcagcgtatt cgcggcactt ggcgcggaac tcagcggcgg tgaatttctc
     6841 ccccggctta ccgtattctt gctctacttt cagttcgatt ggcagaccat gacagtccca
     6901 gccaggcaca tacggcgagt catatccaga aagccctttg gacttaatga taatgtcttt
     6961 cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag gagggccatc
     7021 atgcagaatg aaggtttttt tgcctttttt agctgcacga atgatgccgt acagatcatc
     7081 atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac gcatcgggaa
     7141 ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct cggttccgta
     7201 tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa attcgcgggc
     7261 ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgatgcaa atcgctgctc
     7321 attgcgtatt tttttacgca gcactacttg tatatggcga ccataaaggt ccattgcaac
     7381 atctaacaaa tgcacttcca gttgctggcg aataccggca accgttgggc gtgttccgat
     7441 gtttgcaacg ccgggtaacg gcttttcgcc aaggcccaac acttctaccg cataaacccc
     7501 tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg
     7561 ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc ccagtaaact
     7621 ttctgccaga gccagattgt catccgccag cgcctgacgc acggcggtgc tgctgatacg
     7681 cacgccacct tcgcaaaaag tttgcgtgct ggtgatatcg aagccgtatt ccatacccgc
     7741 tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat cgtcacctac
     7801 ggcaagaaat tttacccgca agtgcttcac cagaagatca ctgatgaagt tttgcgcggt
     7861 taacgccgca aaacgcctgt caaaacgcac gcacagcacg taatcaacgc cacactctgc
     7921 aagataacgc agtttttccc gcagccgggt cagccgtgcc ggggctttat cagtagcaaa
     7981 cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc gcttgcgccc
     8041 ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc
     8101 aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag
     8161 cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca gcggtcaagg
     8221 ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgct atggcgcaaa
     8281 tcatgggaag aaactgaccg cctactgcaa tttttctcgc ggaaaagctg tattcacacc
     8341 ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac attaacggcg
     8401 cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct cggcctttga
     8461 attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat cagctaagaa
     8521 gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg
     8581 tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg ctgcacagaa
     8641 agcatttaac gaaatgcaac caatcgtgga ccgtcaggct gctaaaggtc tgatccacaa
     8701 aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac tggcttaatt
     8761 gcctgttgtt gtcagctttg taaaaaaacc cgctctggcg ggttttttta tgcctgcgga
     8821 agttaccgtt aatcgattat atattttctc catgaataca tcataaatat aattgatgct
     8881 ttttcattgt gaaagataaa atatttcatt tggctgtaaa acgtgaactg tataacaaat
     8941 agcaggaaaa ctgaataaaa aactaattaa cgcaacctta aaacgaaaaa ttataattgt
     9001 cgctcttccc cactttaatt actctcattg ccacaacgtt gctttacatc attaataaat
     9061 acgcatcaag gatagttagc atgtatttgc ttagaaagta aaagtcgttt ttctgagcca
     9121 ggcattcttg ttctggagag aatataatga gttccgtact tgaaactgat aaatcatccg
     9181 catccgattc actgccttta atacaacgga tcagctatgg ttcgctggat gttgcaggta
     9241 atctgctcta ctgttttggc tccacctaca ttctctattt ctacactgac gtggcgggca
     9301 taagccttgc cgtagctggc atcatcctgc tactggcgcg tattgtagac ggtatcgatg
     9361 ccccggtatg ggggattatt attgataaaa cgcattcccg ctacggtaaa tgtcggccgt
     9421 ggtttttatg gttgccatta ccttttgctg tatttagcgc actctcattc tggtcgccag
     9481 atatcagtat gacaggtaaa accatatatt cagcaatatc gtacatgtta gccagcattc
     9541 tttttaccgg tttaaacacg ccgctaagcg caatattacc tttgatgacg ttatcaccaa
     9601 aagaacggtt ggtattaaac tcttaccgga tgaccggcgg gcaaattggc gtgttattaa
     9661 tgaatgcgac ggcgctaccg ttagtggctt ttcttggaaa cggtaatgat aaggcaggat
     9721 ttatttatac tgctatcgtc ttttccgtaa tctcctgtgc gctaacgcta tttgccttca
     9781 aaaatattcg agaactggat accgacaaaa tacagcagga accacgtctg ccgatgaaaa
     9841 agagcttttc ggcaatgaaa ggcaactggc cgtggatcct gatggtgctg gcaaatctca
     9901 tcttctggat tgccttacag cagcgcaaca caaccattgt ttactattta acctacaacc
     9961 tggatcgcaa agatttagtt cccttgatca acagcctcgc caccattcag attttgttta
    10021 tcatcgcaat tccgtttttc agccgttacc tctccaaaac ctggatttgg atcaccggcc
    10081 tgctggtcgc catgttcggc ggaggactga tgtggctggc tggagacaat attcctttga
    10141 tgattgccgc ctggatgctg gcgaatattg gcagtggcat tgcctgctct atgcccttcg
    10201 ccatgcttgg tttcgcggtt gattttggtc gctggaaaac agggattaaa gctaccggca
    10261 ttctaatcgc cttcggcagt accttctgca tcaagatggg cagtggtatc ggcaccgcct
    10321 tcgccgcctg gattatgaac agctttggct atattcccaa ccagccacaa actgccgccg
    10381 gtctggacgg tattagctgg gcgtttatct gggtgccagc cgtgctcttt gcactcgccg
    10441 ccattccact gctcttcttt cgtaaatatg aagcgatgga agagtgcatc caacacgatt
    10501 tgcaagttca taacccttaa acatgtcaca agctcctgga ggaaattatg tcgtttatta
    10561 agcaagatcc ccatcgtctg gtctggcaac aagacgatcg ttatctctgg attgaagcat
    10621 ggggtgagaa cagtttacgt gtgcgtagcg gtcgccatct gccgatgatg cgcaatgaaa
    10681 actgggcatt aacagaagaa aaaggtgatg ctgtcgccta tatcacatat gaagaaaagc
    10741 aggcaacact acaaaacggc aaaatcaccg ccattgttaa tctgcagggt cagatctctt
    10801 tctggcgtaa tgcagaaaaa tgcctgctgc aggagttctg gcgtcagcgc ggcgaaattg
    10861 gcgaagatga gtcggcccac ggtcagtatg tcagcgccct gaatttacag gcccgcgagt
    10921 tcaaaccgat tcccggcggg aaatacacaa tcaaggcgcg gttcgaagcc aacgatggcg
    10981 aaaagttgtt tggcatgggg cagtaccagc agccaaatct ggatttaaaa ggctgtatgc
    11041 tagagctggc gcagcgtaat tctcaggcat cggttccctt cctgctttct aatcgcggct
    11101 atggttttct gtggaataac ccagcggtgg gtcgcgtcac tttcgcgcaa aatggcacgg
    11161 aatgggtggc agaagttagc gaacagttgg actactgggt taccgcaggg gatactcccg
    11221 cagaaatcag cgccgcctat gcacgtgtta ccggtacgcc gccaatgatg cccgattacg
    11281 ccatgggatt ctggcagtgc aagttgcgct accgtaatca gcaagagtta ctagaagttg
    11341 cacgtggcta caagcagcgt aatttgccta tttcggtgat tgtcatcgat ttcttccatt
    11401 ggccgaatca gggtgactgg atgttcgatc tgcgcgactg gcccgatccc gatgccatga
    11461 ttgccgaact aaaagagatg ggcattgaac tgatggtctc cttctggccc acggttgata
    11521 accgtaccga gagttatcgg gagatgaaag aaaacggctg gctggtccac accgagcgtg
    11581 gtttgcctat caatatggat ttccttggca acaccacgtt ctttgatgct acccatcctg
    11641 gcgcacggga atatgtctgg aacaaagcca aacgcaatta ttacgataaa ggcgtgaaac
    11701 tgttctggct ggatgaagcc gagccagagt tcggtgttta cgattatgac aactatcgtt
    11761 actacgcagg gcctgtgctg gaagtcggta atatctatcc gcgtatgtac gccaaaacgt
    11821 tcttcgatgg tatgaccgcg gctggtgaaa agcaggtgat taacctgctg cgctgtgcct
    11881 gggctggcag tcagaaatat ggcgctctgg tgtggtcagg cgatattcac tcctctttcc
    11941 gttctttgcg taatcagttt gccgctggct tgaacatggg aattgccggg atcccgtggt
    12001 ggacaaccga tattggtggt ttccacggcg gtaatattca cgatccgaaa ttccatgaac
    12061 tgttaattcg ctggttccag tggggggtat tcagcccagt aatgcgttta catggcaacc
    12121 gcgacccgca ggttttgcct gaacaaccct atcgtgatgg catagcacaa tgtccgactg
    12181 gcgcgccgaa tgaagtctgg agctatggtg aagaaaccgg tgaaattctc acttcctgcc
    12241 tgcagttgcg tgaaaaactg aaatcttata tcagtaaaat tatggctgag acgcacgaga
    12301 agaacagccc gataatgcgc acgatgttct ttgaatttcc tgaccaggca gaaagctgga
    12361 atatttatga tcagtactgc tttggcccag accttctcgt tgcccctgta atgcacgaag
    12421 gtacacgttc acgtgatgtc tggttgcctg ctggagagac atggatcgat ttttataccc
    12481 atgaacacta cgcgggtggg caaacgcttc atcattgcgc accgctgcac cagataccgg
    12541 tattcattcg cgagaaaggg aaatatcgcc agatatttcc cgtttaaagg acatcgtttg
    12601 ttcattagca cgccccgtat gtagcggggc gtgagtttta atagctatcg tgaggcggga
    12661 gaaaacaacg aagaataatc cgtattgcag attcgctgta ccgccgggtg ctgaatcatc
    12721 cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc aatttctacg
    12781 acagttttat cggcataaaa gtcatatgca taaagcgttg gagcaacgaa gattgcattg
    12841 tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccaag gatttctacg
    12901 tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga acgtcgccca
    12961 ggaatcaaaa gccgccgttc ttccagacaa gccgggaacg gtttttccgg tggtggattt
    13021 gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc ttcctgctgc
    13081 gtagaatcta tcggacagtc ggaaatgatc atatccagtt tatgctgact taattgctcc
    13141 agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tcggctcgcc ttctaccact
    13201 gcggcgttaa gtacgctact gaccagacgt ttggaaagtg catctgccac accaacatca
    13261 aacaataagt tggactcttt gcgatagttg acgatatcca gcatttcctg gcttaaggtg
    13321 aacattttat cggcatagcg atagaccagt tcccccagtt cgctgggttc cagaccacgt
    13381 cctttacgct taaatagttt cccttgcagg cgctcttcca gcgcccggat ctgcccggta
    13441 atggtttgtg gtgttaaata aagcgcctcc gctgcgccaa ccacagaacc ttctttgtag
    13501 acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat ttctctccct
    13561 gataacaatg aaaagggagc cgtttatggc tccccagtac atcgtcctgt caaactgatg
    13621 gacgtaaacg aacgcgtaac cagctatatc caattaccgc cgaagaaatt gaaccgacaa
    13681 ggatacctaa ttttgcccag ttaatcagtt ctggatctac gctaccaaat gccaggctgg
    13741 caataaagat agacatagta aaaccgatac cgcacaggat accaaccgcc ataatttgct
    13801 ggtaagtcgt tccctctggc agatgtgcca gtttcaaacg cagcgccaac cagcagaaca
    13861 gactaatacc cagtggcttg ccaatcagca aaccagcgat gatccctaat ggcagaatgg
    13921 aggtcaaacc ttccagcgtg acaccttgca gtgaaacgcc agcattagca aatgcaaaca
    13981 gcggcaaaat cagatacgcc acccatggat gcaaaacatg ctccagacgt ttagccggag
    14041 agcgcccatg cttctctttt aacggaataa agaagccgac aatgacgcca gccagcgttg
    14101 catggacacc tgatttcaac accgctgtcc acagcaccac gccaaccaga atataaacgc
    14161 ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct acagccgcga
    14221 cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata atgatggccc
    14281 caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac ggaacacgac
    14341 ttcccaacag cgccaacaca ccaagtgcaa aggcaatgtc agtcgccgcc gggattgccc
    14401 agccttcgcg agtgatagga tcggcatagt taaaagccag atagagcaat gccgggacaa
    14461 tcatcccgcc gattgcggca ataacaggaa atgccgcctg gcgcagactg gccagcgaac
    14521 cttgcatcag ctcgcgttta acttccagac caaccaacag gaaaaatacc gccatcagag
    14581 cgtcattgat ccatagcagc atgttcttgt tgatctcaag tgtcccaacc cggagctgaa
    14641 ccggcgtctc aagaaagtcg tgataccatc cactggttgc accgctgttg gccataatca
    14701 tcgctaatac agcggcaata atgagaataa tgcctcccga ggcatcactg ctaaagaatc
    14761 gatgcagatg tttcactttt tatttctctt caggtgaata gatcgttttc acgattttac
    14821 cccgcacgaa tcatcgttaa aatcagatta tagtgtagga atagctcgct ttttacgatc
    14881 tattaaaaga attgcgccat cagcgagaga tgagtttttg atgcccgaaa gcggcggctg
    14941 atgaccttaa accccattaa tcatagtcta atgtcaagat ttctgaagaa attattcgga
    15001 aaagtaataa ttaaattgtt ccatgctcta gttttttatt ccacagatgt ttgaggatat
    15061 ctaatgtaga aacgacattc gcaggagaca ttcggttcaa tgatataagg aaaggatggt
    15121 tctccacaaa gtggataaaa cgcgtcgcac tgttcaatgt tgcatgccgc atctcgcgga
    15181 ctttcttact gccagtgaga ttgcatacag ccattgaata attgcggtta gaaaatgcac
    15241 gccagacatt atttcgtacc ccaccactaa ggaataatcg cggcggaaaa tcaatggtgg
    15301 cgggtgccac accgcgctct ttgacaaagg aaatatggca gcgaccttga tgaatatgct
    15361 gcaaattagc catcaggcag ccgcccaata taaagcattc gtgatcaagg taatgctgaa
    15421 cgttgttgga atgctcatcg gtcagtattc tgaccagttc aaaatgacga tatgccggaa
    15481 gaaccggttt taccaacatt cccggatcgt tttgatgtaa aactgccggg ccgtaatcaa
    15541 tatcaaacaa ggtatcccgc cgcatagtaa gtaactcttt gcgctggaca tgatgtgcca
    15601 gcgtccctgg agacatacgc tcttcatagt tcgatgtgta ttgataatgt tgctctacgg
    15661 cagatgggga gtaattggtt gagatggcaa ccaccctgcc gctttgttct tctgcggtaa
    15721 caagagcata aagagagtta ttgctaccat tatacttgac gcgaaaagcg cgggtactca
    15781 gggtaatatc agaagctggg aaacactgtc gactttcttt atagtttgct tcacgcgcta
    15841 attttaacag ttcacggcta agtccagtgc tgttaagaag taatgacttt ctgatatccg
    15901 caagtgcctc cccttgctca atcatcaatg ctaactgggc tcctcgtggt gtggtaatta
    15961 catgttccag agtgataaat gttttctcac aatgatggca atacattctt ctctggcctt
    16021 gtgcagaata tccatatttt ttgagggacg tgcctccgca agttgaacat tggcaaatca
    16081 aacctctcca ggagtgattc acaatattac gataaatatt aagcgactgt tcagatatca
    16141 ctggaaacaa gtaaccacat tcacggcata aaatattttt accctgtgcg acataatcct
    16201 ggctattcag caaccccagg tttttacatc ctggtgtttt gcaacaatca acatttacgt
    16261 tggtaaacat atccactccc tgcgaatagc cgtatcttga tattgttcaa cgcgaaaaga
    16321 acctgattca atgcgtagca ctgaatcagg cgggcatagc cctaaatggc atacattaca
    16381 tttattatga ttatcaggtt agccccaggt caccgcttct ggtttatcac ctctggcttt
    16441 cattagagaa tcattatcaa tatcagtttt atattttaaa aacttaccat acatctgttt
    16501 ttcaatatct ggttttttac cgatcagatt aagtgtttca taacgatcag atttcagatt
    16561 gtagagatat ttcggtttat tattgcgatc gataatcatc ttccagtcac catcacggat
    16621 cgcccattca tcggttggat catcctggaa tggcatatca atcccgaaaa ttaatggctt
    16681 ttcgcgtttc aatgcttttt gctcaagaac aggaaccagc gattcaccat cgaaagtacg
    16741 gtctgtaggt aatttgaagt tcatcatttt cgctaaagta ggcatccagt ccagaccata
    16801 aacgggtgta tctgaaacca ttccctgtgg tagatgttta ccatatttaa taatggctgg
    16861 aacacgaatt ccgccttccc aaaggttatc cttgcgaccg cgtaatccat ccgtttcccc
    16921 tgccaaattc agctcataca ctttgcgcgc ttcacgcgtt accggaccgt tatcactggt
    16981 aaaaataacg attgtgttat cttcttcacc cacagctttg attttatcca gcacttttcc
    17041 aacctgtgca tccagatagc tgatattggc ataatattcc cccacaccac gccagggttt
    17101 gtctgcccag tcgccataaa ataaatcagg atgctgcttc tgatacgcgc tcatatattg
    17161 tgagtacata tcgaggtatt ttttgggcga agccaggggg ctatgcactt cggtaaaagc
    17221 aacatagagg aagaaaggct tgctgtcctt tttgttatcc agccagttga cgacttccga
    17281 actgacatac tcaccgctca ttttatcggc tcgtggagtg ggttgcccgt tacgtagcca
    17341 gcctgtcggg taaaccatgc cataacgcgg gcgttcttta gcgttatcca gcgtggcgtc
    17401 ggtaacaaag cccgccgtat taaccagtga gtaatcaaag cccatatctt gtgcttgtgg
    17461 ctgatcggtg cgatcgctgc ctgcattcag atgcagctta cccatcattg ccgtgtcgta
    17521 cccttgcgct ttgagtagat tagcaatcgt gagttcgtta cgccctaagg caacatcttt
    17581 gcctgaagga atccatgagc gaattccagt acgaaatggc atccggccgg ttaatagccc
    17641 tgcgcgtgaa ggtgaactta aaggagcggg ggcatagtag tcagtaaatt tgaccccttc
    17701 ctgggcaagc ctgtcgatat taggtgtttt aacgatctga tgaccatatg ttgctaaatc
    17761 gccataacct aaatcatccg ccataataat gactaaatta ggttgtttag cctctgcggc
    17821 taaggcagga ctaaaagcat tccctgtgca aactgcaagg ccgatcaaac tggccattaa
    17881 cgttttctgc ataattattc cctgataaaa aattagaaat cataaccaag catataaatc
    17941 atttgatcgc caaagccatc gtagcccagc ttattatcga aataacgcca cgtcacactg
    18001 gctttccagc gaggatagaa cttccaggca atagttaaac cgccatttaa cccgttccgg
    18061 ccaaattgct gctccgtgta agcatcgttc ctgtccagtt ctatttcatt ccagttagat
    18121 aaaacaaatt tttcgtcaaa taacgtaaat ggtaatactg ctgtccagcc aacaacataa
    18181 ccattccaac cattcgtttg accatattta gctgatacgt agtcaccaga ttgattatgt
    18241 aatccaatat acggtttaaa aaagccccac tcgccgttcc agccgaggta accgaatcca
    18301 taaaacatgt ccagatcgtc accccatgag ttatcccatt ggccataaat tttgccaaag
    18361 aatgtcatat cagagtcaaa gagtctgaca tgcgtcatta ctgaaaccgt atgatttcgc
    18421 cctgctacag catgattgag tacattactt tcataaaatg agtaaaattc tgcttcttta
    18481 aaatttaccc caaaatccgc agtcactttc catacttcac cacggtgagt gtttacaaat
    18541 ccactattcc agtctgcata acccgcattt attga
//
LOCUS       DAPQZD010000088        18521 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPQZD010000088 DAPQZD010000000
VERSION     DAPQZD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.088"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..358)
                     /locus_tag="RO454_002961"
     CDS             complement(<1..358)
                     /locus_tag="RO454_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDY0934762.1"
                     /translation="MTSQSVLPPNAITPERALEVVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPRDDPYTFAIKI"
     gene            complement(361..1281)
                     /locus_tag="RO454_002962"
     CDS             complement(361..1281)
                     /locus_tag="RO454_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDY0934763.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKGSDLDQIGANFDCL
                     RLLITPANPDVIPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGTPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLAIYVDTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDVIAGVGQAPYCTEIKLSVQMG"
     gene            complement(1278..1619)
                     /locus_tag="RO454_002963"
     CDS             complement(1278..1619)
                     /locus_tag="RO454_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDY0934764.1"
                     /translation="MLGMDRNTGKLLSGTDHIRQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            complement(1622..2524)
                     /locus_tag="RO454_002964"
     CDS             complement(1622..2524)
                     /locus_tag="RO454_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDY0934765.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDDKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVQDRQGTITEV
                     RTTYNGHTHICPDGETQQPNQPMA"
     gene            complement(2505..3041)
                     /locus_tag="RO454_002965"
     CDS             complement(2505..3041)
                     /locus_tag="RO454_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934766.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDESVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWINGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHEGDYYGPIGR"
     gene            complement(3038..3718)
                     /locus_tag="RO454_002966"
     CDS             complement(3038..3718)
                     /locus_tag="RO454_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934767.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMVYNRALNRTA
                     KHMHRISAGMILTALAAKNHKAVDKRIKPFIKRRNFTKEGAGDLSSAKLWYGLNDFRV
                     SELKGRLQNPRRQKQPRDLETGLFLKTKKGARGATFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLDRGGIEEARVPVHDALEDAIDDYIFENIGPVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            complement(3750..4130)
                     /locus_tag="RO454_002967"
     CDS             complement(3750..4130)
                     /locus_tag="RO454_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934768.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIEPDGSVTWSGWTDYVVKEPQP
                     DGTGLTNIFLEPHTSSENSEYSKY"
     gene            complement(4127..4546)
                     /locus_tag="RO454_002968"
     CDS             complement(4127..4546)
                     /locus_tag="RO454_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDY0934769.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNTASGTDNELAQSIASAIVSQSQEQELAPATRRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            complement(4581..5615)
                     /locus_tag="RO454_002969"
     CDS             complement(4581..5615)
                     /locus_tag="RO454_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDY0934770.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYLDPVTDTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGISEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            complement(5674..6003)
                     /locus_tag="RO454_002970"
     CDS             complement(5674..6003)
                     /locus_tag="RO454_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDY0934771.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATVG
                     TFKVWDGSVGKAIGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPISVG"
     gene            complement(6003..7310)
                     /locus_tag="RO454_002971"
     CDS             complement(6003..7310)
                     /locus_tag="RO454_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDY0934772.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPTYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYNGIAKRLQQAISDPDVRGVLLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPGDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKMERFMSGTETTAETTTTEQTATTTTVAQVEFNTEQIR
                     ADAASSELARVMAIINCPEAVGREAQAKALAGVPGMTVGQAQVVLAAAPKTAQARTET
                     ALDTLMSTESPATIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            complement(7310..8884)
                     /locus_tag="RO454_002972"
     CDS             complement(7310..8884)
                     /locus_tag="RO454_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0934773.1"
                     /translation="MTAPQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSAD
                     AALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYLGISREDA
                     RALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESSAG
                     SVFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCR
                     RIPAQLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMY
                     AATIESELDSQQAFEYIAGAGTDIDSNPLNSFIQSYVTYYNGANIKLGGVKVPHLHPG
                     DKLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWR
                     FFMGRRRFIAARQASLMFCCWFEEALARGIITLPRSAVRSFYEARNSWTNALWIGAGR
                     MAIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSW
                     SVSHPSNTNDNGWGGN"
     gene            complement(8881..9114)
                     /locus_tag="RO454_002973"
     CDS             complement(8881..9114)
                     /locus_tag="RO454_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934774.1"
                     /translation="MTSREVLTERLLEAEIALHKLLTGKSTVSLSHGDSAGNNRSYQY
                     SQASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            complement(9114..10976)
                     /locus_tag="RO454_002974"
     CDS             complement(9114..10976)
                     /locus_tag="RO454_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDY0934775.1"
                     /translation="MTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTNDAIKI
                     VSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAEMPVI
                     QKIFPDWDKSNERNNLQRKEMSGAILDFRGASAPGNFRRLTKQVVEGDEVDGWPLEVA
                     KKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYLTCPH
                     CGEEQVLVFGFDGIEYGLKWDNSLQTNEAKSSSAYYQCCHCPEHFYYRDLEKMEFGGR
                     WIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKAKGDP
                     LKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYITGGIDSQTSGRY
                     ECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSRWAWD
                     TGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTDTAKD
                     LLAMRLQLEPDSKSATPGAIHFPNDDEIFSTTEAKQLVSEVLIPKLINGRVVYRWDNQ
                     GRRNEALDCWVYGLAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS"
     gene            complement(10963..11529)
                     /locus_tag="RO454_002975"
     CDS             complement(10963..11529)
                     /locus_tag="RO454_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934776.1"
                     /translation="MASQAEVAAHLLLSDRRLRDLAKLPGAPVPQGRGDWELDAWRHF
                     YIRYLRSNRRDTTSNDEPEAGDNSPEKNREQWLKNEERQERILMARVKRRILAKRYAP
                     IELISVAVSRVAVELRTRVESWPPRLKKVWPEMPQEASSVLREELAIALNELSDIRID
                     FSDYDVSDIERDLDRVESLARDDTDDGC"
     gene            11898..12143
                     /locus_tag="RO454_002976"
     CDS             11898..12143
                     /locus_tag="RO454_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934777.1"
                     /translation="MNREKIEALMSVILKEADPKKFPEKLSEDAIVSFYRANSSYLNC
                     STLREWLSGEAEPSDEILQKIMVQNNWRATKWSNQLP"
     gene            complement(12203..12397)
                     /locus_tag="RO454_002977"
     CDS             complement(12203..12397)
                     /locus_tag="RO454_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934778.1"
                     /translation="MKKLLLAVAFFTLAGCTHSTYTEATRADGSSIKHVMIAPGTKIT
                     TANGGCIDSTGAEASCLGGK"
     gene            complement(12405..12884)
                     /locus_tag="RO454_002978"
     CDS             complement(12405..12884)
                     /locus_tag="RO454_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HDY0934779.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERAGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADEVNIKAFPRSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            complement(12884..13156)
                     /locus_tag="RO454_002979"
     CDS             complement(12884..13156)
                     /locus_tag="RO454_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDY0934780.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRIFYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            complement(13156..13539)
                     /locus_tag="RO454_002980"
     CDS             complement(13156..13539)
                     /locus_tag="RO454_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934781.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            complement(13652..14323)
                     /locus_tag="RO454_002981"
     CDS             complement(13652..14323)
                     /locus_tag="RO454_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDY0934782.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMSDFEFADSSWRRSVNSLDASQQ
                     AWLRYCYGGNLAFKHQTAICEAVWSRYKGNIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSTLAGMLSVSRSTWCETYSLHWVGMKEAVRALDEVALLATLHHYQNHL
                     DDVCV"
     gene            complement(14323..14616)
                     /locus_tag="RO454_002982"
     CDS             complement(14323..14616)
                     /locus_tag="RO454_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDY0934783.1"
                     /translation="MKKIDLRKAARSRACTVRIPGVCNHNPETSVLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEHDSLLLMHAEGVFRTLAIWRDEEFI"
     gene            complement(14613..15209)
                     /locus_tag="RO454_002983"
     CDS             complement(14613..15209)
                     /locus_tag="RO454_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDY0934784.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPAERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGSISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            complement(15287..15466)
                     /locus_tag="RO454_002984"
     CDS             complement(15287..15466)
                     /locus_tag="RO454_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934785.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
     gene            complement(15618..16259)
                     /locus_tag="RO454_002985"
     CDS             complement(15618..16259)
                     /locus_tag="RO454_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934786.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTVVAGHPEIDVSELDIGPLYRYYKRGEKGNPLADLLIELAPPTCERVRISPEVY
                     IIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLIPIYRSDK"
     gene            16503..16736
                     /locus_tag="RO454_002986"
     CDS             16503..16736
                     /locus_tag="RO454_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934787.1"
                     /translation="MGTLGNQPCRSRYEFDDFNLANRAAALTKLAKETGVSVDTLIKL
                     EIAMQLNRLNSILIENGDKMDENLAGIGEILQK"
     gene            17135..17623
                     /locus_tag="RO454_002987"
     CDS             17135..17623
                     /locus_tag="RO454_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDY0934788.1"
                     /translation="MATNPREHARRVLDTFWCGRGFPVDPAKIAHDMGLDVFITDLPG
                     KVSGALIKQKDQDPAIFLNSDDNKVRQRFSCGHELGHYIARQASHSDEYEYVDLRGET
                     ASAGTHPDEIFANKFAAELLMPVDEVRKLHADGQPSYIMAHYFGVSDDAMTYRLKNLR
                     LG"
     gene            17633..18238
                     /locus_tag="RO454_002988"
     CDS             17633..18238
                     /locus_tag="RO454_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934789.1"
                     /translation="MPSDDDFNSRFKQEVARRLKDSLNLHRQLAEEVEPEDDETPVQS
                     PDEASPQPKVSHAPKKTAVRIRGIARKRAAEARKMAVAASSKRIPTATPSQQVTRTES
                     KILTEFTSVKQVNSLDTEHERKQLNNKDFEAEIALKKRYGKWFLIILAFQLLIMNGVF
                     IADGAGKLEFEDLTLQLYMGGTLTEVFGLVLVVTKYLFKRK"
BASE COUNT         4476 a         4889 c         4431 g         4725 t
ORIGIN      
        1 atattttaat tgcgaatgta tacgggtcgt ccctgggcgt ggcttcaaac cactctctca
       61 ttttgctctc aaagccaaca gttaataacg ctcgttcaac agctccacgc gttccgcggt
      121 gcttattctg ccacgcagct gctttaataa tttcgcgttt ttgctgctca ctccagtcgg
      181 ggttccagta ggtgaccgcg tactcccacg ccagccacgg caacagattt gcagggcaca
      241 aatcgggatt tttaataatt cgaatatcgc caggcagatc gccgacgtga gataaaacaa
      301 cctcaagcgc cctctcaggg gttattgcgt tcggaggcag tacgctttga cttgtcatgg
      361 ctaccccatt tgcacgctga gtttgatttc tgtgcagtac ggagcctgcc cgacgccagc
      421 gatcacatcc tctgttggcg atatcagaat cacacgttca gtgccgtcac gatgtatcgc
      481 gtcgtaaatt cctgacagag ctgccgtcgc tccgatttta tgttgcgtat ccacatatat
      541 cgccaaatcg ctctgagcac cgttaagcaa aacgttctgg tcgggtccga gcccggctac
      601 gataacggcc tcaacccggt aattcaggtt cgacgcaggt tttacagtca cgaaatcagt
      661 cagcggtcga gtatcgtcag gagacagggc cgcattcact gtattcagta gtgattgagg
      721 cggagtgccg tcgccggttc gcgacaagac atacatatcg acgtatccag gctgggtcac
      781 cggcgggccg tacgcctgag ctgacagaac gtcgggatca ctcgacagcg cgaaatagtt
      841 ataggcgttc gtactgccgg cagtattccg cgcataccat gatagctgta ttcggtgtcg
      901 atactcgtcg tcactctcgt aaacagcctc agtcggggga attacatcgg gattagctgg
      961 cgttatcaac agccgcaggc agtcaaaatt agcgccaatc tggtcgaggt cactcccctt
     1021 tgcaaacgcg agcaatacag cgagcatccc ctgatttacc cgagctgtaa taatgatttc
     1081 tctgtatgca actatttcca gcagtttaac ggccgggtct gactccagga gtgcgttaaa
     1141 ttgtggatat aatatctgca gctctgacag tagattagat ttaacatctg aaaataacgg
     1201 ggttttaacg aatgctggcg gcggcagcag actcatatca atagttttca ctatcgaatt
     1261 ctgaactgtt gatgttatca aaatatcagc tccatattat taaactctaa tcgctgttgt
     1321 gtttcgatat ctgttgcgat aattaatatt gttattttcc cgatatccac tgctaacaca
     1381 tttattctgt caattctgac gcgtggttcc catcgtgcta ttgcgccagc gctttccatg
     1441 attattcgca tagcaagcga cggatctgtg ggattatcaa caagatcaaa taatttgctg
     1501 ccatattccg gcagcattac acgcgtcccc agcggggtcg ttaaaatatc aacgatagac
     1561 tgacgaatgt gatcagtccc cgataaaagt ttaccggtgt tacggtccat tccaagcatc
     1621 tttacgccat tggttggttc ggttgttgag tttccccgtc cggacatata tgagtgtgtc
     1681 cgttatacgt tgtgcggact tcagttattg tgccttgtcg gtcctgcacc tcttgccctg
     1741 ctaacacact accgcgagtt gtgatattac ctgttccacc gttatctcca gtgacagaaa
     1801 tatcgttacg gaatgtcgct aatttatcta ctactaatgt ctcggtaata tgcgttggac
     1861 cagctagtgt tataccaccc ggagcccacg cgctgattgt ttgagctgtc agcgatattc
     1921 tgttcgcagc agtaacacta acagaactcg ttgctataac gttcactaaa tcagcatgtg
     1981 cgttaattgt atctgtcgct gtgacattaa taatatcagc gtctgcgtta attaatttcc
     2041 caccatggat attaactaca ttttcgctat taatagtaac atctgcgcgt ccaaatattt
     2101 ctatttcaga ctcaccgagt atttttacca cgccgtccgg tacgccctgc catgtcagag
     2161 tgtgattttt tgtgttatac gtcatcgtag ccccgtcaca gtatgcagtg acatgatcat
     2221 gggggtcatt gcttggcgga gctttgtcgt caatgtgcag cccgagcata actacaccgt
     2281 tccgcgtatc cccaccctca gaaacaacag taacgggatc gcccactgat ggtgcgctcc
     2341 agtcgacgcg ttctgaggtg gcgtgagtaa accactgcag ccagcctgac tggtgttcgc
     2401 caccaaatga gactcggcag cgtggaggat tcattttaac tgcagctatc gtgccgcgtt
     2461 taactgcgtc acgcagcctg cgcgcgttct ccgctgcagc atattcatcg tccgattggg
     2521 ccataatagt cgccctcatg ttctgcgccg atatctggtg ctattccgac gtatatattt
     2581 ttcagggtcc cctgtgaggg ataatcgaaa atatctggac caacgcctac aacctgcgtg
     2641 aacgacacgc actgaactga atgcgaaccg actgactgcc cgtttttaat ccagtcgcac
     2701 ggttccgcgt cgctgaattt tgccggttta gttccgggac cgaacattcg cccattgatc
     2761 cagctcgtca tatagagcgc tgcattctgc gatttcagtc cgtactgatc agctgcaaat
     2821 tcacgcagca aatacaaatt acacgacagc tcgactgact gaattgagcc tggaaccgat
     2881 tcgtcactct gtgaccagtt gctgatttcc aggaacagag ctggcgtttc aaatccagcc
     2941 ggaatttccg gataaattcc gaatgttttg atgaacggta tttgtaaaac cgcgccttta
     3001 acgcgatcaa gatattcgtc aaatgcatcc agtccgctca taatcgttta cctgtttttg
     3061 ggtctacgtg gacattacct ttcacgcgac cgcgcaaatc tttctcaaaa aagcccataa
     3121 aaacagggcc aatgttttca aaaatataat catcgatagc atcctccagc gcgtcgtgca
     3181 caggtacgcg agcctcttcg attcctccgc gatccagtcg aatccagaca cttttcgcac
     3241 cgtagcgttt cgctacgaat gaatcgggcc aactcatcat cgccagccct gcacttttgg
     3301 gtgtaaaagt tgcccctcta gcgccttttt tggttttcaa aaacaggccg gtctcaagat
     3361 cgcgaggctg tttttgcctc cgtggatttt gtaatcgccc ctttagctca gatacccgga
     3421 aatcattcag accgtaccag agcttggcgc tgctcaggtc tcctgcccct tcttttgtaa
     3481 aattacggcg cttaatgaac ggcttgattc gtttatctac ggccttatga tttttagctg
     3541 ccagtgcagt caaaatcatt cctgctgaaa ttcggtgcat atgttttgca gttcggttca
     3601 gcgccctgtt gtataccatt aacatttgat gttgcgtcgc gctgatttcg actctgagct
     3661 gctccagcgc cgaaacatcg atatcgaaca tctgcgcgtt actacgtaaa tcggccacgg
     3721 ttccccctta agtgggaccg aggtcccact tagtattttg aatactcgct gttttcgctg
     3781 gacgtgtgag gctctaaaaa aatattcgtg agccctgtgc catcaggttg aggctcttta
     3841 accacgtaat cggtccagcc agaccaggta acagagccgt caggttcgat gctgcgctgc
     3901 tttggaacct ggacgacgtc acgcgcaaca acgccagcga cgtcgtccga gagcgccgtc
     3961 aggctcgtta ctgtaccggt aataaaacca gcgtgaggaa cgtcaacgcg cgcgtaaggc
     4021 tcgttgaaaa tagcgataat ggggtcggtt ttaccgcgta atttaacggg ccgtccccac
     4081 tcacggatca tctcctcgtc gccggcccgg aggtcatcgt agtagctcat agagcgtaaa
     4141 cgtgatcagc tgcgataagg tctgcgacct ccggtgaatc aacaacaatt tcacggcctg
     4201 cggcaacaat ctcgcgaacg cgacgcccgt tcacgtagtg ataaacgtcc agcgtgttac
     4261 gcaacttgac gcggcgcgta gccggtgcga gctcctgctc ttgtgactgt gaaacgatag
     4321 cgctcgcgat tgattgagcg agttcattat cggtcccgga cgcagtattc actgtaatct
     4381 cgatgcctcc cgcgtcaccg gattgctcgt tcagaatatc gagctctgac tcagcgccgg
     4441 caacgattga ttccagttcc gcaatagtcc cgctgcgcgg taattcgcga ccgagctgag
     4501 cgctcagatc gtcaatacgc gccagcaatt ctgtttttgt gctcatgttg attccttaac
     4561 aaaaaagagc ctttcgactc ttacgcgatt tttacgacaa cgaacgcgtc ggcgtccgtg
     4621 aggaccattg ctggcgcgga ttgcgtcatt gtttgcgtca ctgccggatc accggtagtg
     4681 gtccagactt ttggatagcg cgtcgcttcg gaaataccct ctttcagcgc gtcttcgtcc
     4741 tgaattgcgc cataagtgcg gagcccgcgg ttttttgtgt tgccaaggat catcgtgtta
     4801 tccggcatat aacgcgtttc agtgtctgtt accgggtcga ggtattgccc tttgtagaca
     4861 aacagcgcag tgtcgccgta gtagccttta aagctgacaa cgtcgcccag gtttttcagc
     4921 gcgacctcga gctggctgtt tgagccacgg cgcgtatcca gtgccgtcca gaatttttta
     4981 aaacgcttca actgcttcca ggctttacca tccatgatga tcacgttaac cgcaccggag
     5041 gcgaggtccg cgtaggattc aatatcgtcg ctcgggtcat acgtttccgc gtcctgagtc
     5101 gaccaggctg tcgaaccgga ctgagtgacg ttattcgtcg cgctgcggct catgtcgatc
     5161 tcataggtct cgatattgct gccggaaaca gtgtatttac cgtacagaac agcctgtacc
     5221 gcctggaact cttcgagctg gctgatgctc agttcctcat caagcaggtt ctgcatgatg
     5281 atttttgcac gacgttcgac cggcgtttcc ggctggccaa tctgctcacc ggctgcgcgc
     5341 ttgatgctca tattcggatt gatcgtgtgc ttgggtttcg tgtaacccgg tttgaaatgg
     5401 ttcgttgaat agccgcgagt gcggtcaact ttgccggtga tcatcggcgc gcagtacacg
     5461 gccatgttca cttttcccgg gattttatcc agaaaaacct cttcgctcgt aaacgtgtag
     5521 gtctcacgga aaaacagctt taaaaacaac ggattgaact taaatacctg ctgtgtcgcg
     5581 gtaatcagtt ctgacgtagt aaacgaatcg ctcatttata tatcctgtta taaaaaaacc
     5641 gcccggaggc ggcttgtttt gagttttttg ctatcagccg acgctgatcg gcgtacccgc
     5701 aaatgctgat ttgcgtttag ctacgtcggt aaccgtaccc cagttaatcg ccgtgtaacg
     5761 gaacgagcct gatttatagt acgagcagtc tgcagaactg gagccagtat taactgcggt
     5821 ggcagtgagg ccaatagctt taccgactga gccgtcccaa accttaaacg tcccgacagt
     5881 agcgtcgagc ataatcggtg tcagccgggg tacgttgatc ccgccctgaa aatgaccgac
     5941 ggtcgtgaca acaaggtcag gcccaagaat aaaatcgtcc gggctatacg tttctgtagt
     6001 cattattcat cccctaaaat tgaacgccct gcagcaacca gcatcgagac gtttgcggtt
     6061 gttcctgttg ccgtagtact gccagcatcc tgaatagtcg ctggtgattc agtgctcatt
     6121 agtgtatcga gcgccgtttc tgtccgcgcc tgcgccgttt taggtgctgc agcgaggact
     6181 acctgcgcct gcccgaccgt catcccgggg acgccagcga gcgcttttgc ctgcgcctcg
     6241 cgcccaacag cttcgggaca attgatgatg gccatcacac gcgccagttc gcttgatgcg
     6301 gcgtccgcgc gaatctgctc cgtgttaaac tcaacctgcg cgacagtagt cgtggtcgcg
     6361 gtttgttctg tggtcgtggt ctccgccgtg gtttctgtac cagacataaa gcgctccatt
     6421 tttggtttca gtgcgtcggc catcactgcg atagcgtccg cgtaattaac gagttgatca
     6481 gcgagaccag atttaatcgc gtccgcgccg ataaatactg cagcttccgt ggccagcacc
     6541 ctggattttt tcagcccggt ataatccgag actttttgcg cgaactgctc gcgcgtgctg
     6601 ttaatactca gctggaattc gtcgcgaacg tcgcctggca gctgggaata cgggttcccg
     6661 tcaactttgt gcgcgccggc gtaaatcagc gtcacgtcga cgccagcaat ctccagcgct
     6721 ttttcgacgc agcggtgagc catcaggaca ccgattgaac caactgtgcc tgtctgcgtt
     6781 atcagccggc gcgaacacgc tgacgccagc aaataggcag cgctgcaggc cgtatcgctg
     6841 gccagcgccc acacaggttt ttgctctcgc gcccgggcga ttaaatcagc agtatcaaac
     6901 gcgccggcga cttcaccgcc aggggagtca atatcaagca ggacccctct tacatcggga
     6961 tcagaaatcg cctgctgcag gcgttttgcg attccgttgt aaccgctcat cccgctaact
     7021 gggttgatat aacccaattt atgaaccagc gtcccggtaa ccggcagaac ggctatacca
     7081 cgctccacgc gatacgattt ctggcgtgtt cgctcgctgc tgtcccaccc catcgcgagc
     7141 gcgttcattt cgtcgctgtt cattacctct ccagacgctg tatcaatcag tcgcccggta
     7201 ccgaaccggt cgctcagcgc cgaaaaaaat acccgcgcgt aggtgggctc cagcaaaagc
     7261 ggttgattga acgccctggc ggcgaggtgc ggaaaattgt tccacggcat cagttacctc
     7321 cccagccgtt atcgttggta tttgaggggt gagatacaga ccatgacggt tcgctcagcc
     7381 ccatttcacg ccggcgctga atttcgtatt cctgctgctc cataacctcc tcgtaatcct
     7441 gtccctgcag cgccagttcg ttctgatacg tgctgaggcc agttgtgatg cgcatggcgc
     7501 tctcctgaac ctctttcagc ccatcaatcg ccatgcgacc agcgccgatc cagagcgcgt
     7561 tcgtccagga gttccgcgcc tcataaaacg accggacagc tgagcgtggc agggtgataa
     7621 ttccgcgtgc cagcgcctct tcaaaccagc agcagaacat cagcgacgcc tgtcgcgcgg
     7681 cgataaaacg tcgacgcccc atgaaaaatc gccagctgac gttagcactg gcccgggcgc
     7741 tggagtaact gacctggctg tagtcccggc taagttcttc gtatgacgcg ccaacgccag
     7801 ctgcaacgta acgcaggagt gatttttcca gtgaactaaa accagcatca gcattctgag
     7861 cggtctgtaa actgagtttg tcgccgggat gcaggtgggg aactttcaca ccaccgagct
     7921 tgatatttgc gccgttgtag tacgtcacgt aactctgaat aaacgaattc agcgggttcg
     7981 agtcgatatc agtacccgcg ccggcgatat attcaaacgc ctgctggctg tcgagctctg
     8041 attcgattgt cgcggcatac atcgccttca caatggcgct ctgcagttgt gtttgctgca
     8101 gtgtgtcgag cattttcagg cgctccatga cgctgtaaaa aatattgtcc ccgcgggtct
     8161 gtccgtcctc gagcggctcg aatatgtgga tgaatgcgtg tctgccgctg ctgagctgtg
     8221 ctgggatacg ccggcattta ccgactccgc cgagagggta agtatcctcg gctatccagt
     8281 acccgaccgc ggccccgttt ttatcgatat caacaccggc gcggcgaaat tgagtgtcag
     8341 ctgcgtaacc cggatttcga atccgtttcg gtgagaccat tttgaatcgc gtccgaaaaa
     8401 cgctgccggc actactctcc cagactggtt gtacgcaggt ttcaccgttg aaagcgtgag
     8461 tagccacgcc ctcgcggatc atcattgtga acgtccgttt ccgctcgata tcaatcgtgc
     8521 agtgagggtc ctcggcgtat tcagtccacg ctacctcgac gtcacgcgcc agcgccctgg
     8581 cgtcctcgcg agatatgccg aggtaacgcc agttagggcg ataactcaac ttaaacagat
     8641 tgccgacgat atgatcctgg tgcagctgca cggcgttcga cgcgacgccg ttattgcgaa
     8701 cgagatcgtc tgctcgcgcg ttaccgcgat aaaaattcgg caacagtgct gcgtcggcgc
     8761 tctgctgtgg tgcgttccag tcaatcagct ggccaccgaa accgggaccg ccgccgttat
     8821 accccgcgta acggcgtagt ggcgttttac cgtcaggccc gagcagctgc tgcggagcag
     8881 tcataatcga actcccacgg gccgacggcg tcccgtactc agaccgagct gtgatttcag
     8941 ctcgataatg tacgttcgga gctgctcaat actcgcctgc gagtattgat agctccggtt
     9001 atttcctgct gaatcgccgt gagacagcga cacggtcgat ttacccgtta ataatttgtg
     9061 cagagcgatt tccgcttcca gcagtcgttc tgttaaaacc tcgcgcgacg tcattagctc
     9121 cctccgagca ttctggccat ctcctcgaga gacagtttgt tttgtgattt tttccgttgc
     9181 tcagcgagcg tctcgagatt gagctggaaa cgaattttac tgatacgcag cgctgccagt
     9241 ccgtacaccc agcagtcgag cgcctcattt cgccggccct ggttgtccca acgataaacg
     9301 acgcgaccgt taatgagttt cgggatcaga acctcggaga cgagctgttt tgcctctgtc
     9361 gtgctgaata tttcgtcgtc gttgggaaaa tgaatcgcac ctggtgtcgc cgatttggag
     9421 tcgggttcca gctgcaggcg cattgcgagc aggtctttcg ctgtatctgt cccaatcaac
     9481 gacaaataca ctttattcgc gttacgggta cgaggcatat ttacgaccgg ctggccgtat
     9541 gagctcgcgc ccttgattgg aatgacccac agcggaccga gtttcagcga gcggttataa
     9601 acgacctgcg catctatacc accggtatcc cacgcccagc gactgacgcc gattgtggtc
     9661 ccgtcgctgc gccggtattg tttgcgaatc actccgtcga cgcgctgcag cgtgtcctcc
     9721 tcgtcgtagc gaccgaggac gattgtttta tcaatcagcc agcactcctc ctcggctccc
     9781 cagccccaca cataacactc gtagcgcccg gacgtctgag agtcgatgcc gccggttata
     9841 taaacaacac cgtcaggaac ctggctggcg tatttttccc ggcgattaac cagaatatcg
     9901 tgctccagct gctcgctggc cacgtcgctc cagagttcgc cgagcgtcgt gttatgaaac
     9961 gtcttttcct tgagcgggtc gcctttcgct ttcagccact cgctgacaat ctcgccccat
    10021 ccatcgaggt ttagtgaata cagggcgttt atcacgatcg cagcgtgttt cggcgcgcga
    10081 acgacgccac cgtcatgatc aaaaaaatga atgccgtctc gggtccaggt gcagtcctcg
    10141 gctatccagc gccccccgaa ctccattttt tcgagatcgc gatagtaaaa atgttccggg
    10201 cagtggcagc actggtaata cgcggacgac gatttcgcct cattggtctg caggctgtta
    10261 tcccatttga ggccgtattc gataccgtcg aaaccaaata ccaggacctg ctcctcgcca
    10321 caatgcggac atgtcaggta aaaacgaaac gtcagatcgg cagcgtcttc gagcatttcg
    10381 atatgacttt tgccggtaac ggtcggcgtc gaaccaaaaa tcgccttcgg gtacgctgcc
    10441 cctttaattc gaacgagcgc cagttcgatg ggcgagccct cgcctttgcc ttttttggca
    10501 acttccagcg gccagccgtc aacttcgtca ccctcgacaa cctgtttcgt tagtcgccgg
    10561 aaatttcctg gtgcactcgc gccgcgaaaa tcaagaatcg cgccgctcat ttctttacgc
    10621 tggagattgt tacgctcgtt gcttttatcc cagtcgggga aaattttctg aatcactggc
    10681 atttcggcga tagcggggtc aacctcgtcg gcgacaaatc catccgattc gtcatcgata
    10741 ggctgataga ccacggcact acgttttttg tgctcagcga aatagagcag cgccgcgacg
    10801 agtatttttg tataaccgag acgagctgat ttgcgaacag acacgatttt tatcgcgtcg
    10861 ttcgtcatca tattgagcat cactacctgg accggctgag tcgtccagtg cccagcgatg
    10921 tggctggagc cctcagggag ataaaaatat ttatcagccc actcaacacc cgtcatcggt
    10981 atcgtcacgc gcaagggatt caaccctgtc gagatcgcgt tcgatatcgc tgacatcgta
    11041 atcgctgaag tcgattcgta tgtctgacaa ctcatttaag gctatcgcca gctcctctcg
    11101 taatacactg ctcgcctctt gcggcatctc cggccagacc tttttcaggc gtggtggcca
    11161 cgactcgacg cgggtacgta attcaacggc gacgcgagat acagcgacgc taattaattc
    11221 aattggcgcg tagcgtttag cgagaatgcg gcgttttact cgtgccatca ggattcgctc
    11281 ctgtcgttcc tcatttttca gccactgctc gcgatttttt tcgggagaat tgtcccccgc
    11341 ttccggttcg tcattactag ttgtgtcgcg tctattgctc cggagataac gaatataaaa
    11401 atggcgccat gcgtctagct cccaatcacc acgtccttgt gggactggtg ctcccggaag
    11461 ttttgcgaga tcgcgcagac gacgatcaga tagcagtaaa tgtgctgcaa cctcagcctg
    11521 acttgccatt aaaaatcctc accggaaccg gaaataccca aaatgaaaaa atactaaaaa
    11581 cgagcgagtt tttgcgcgtc taccgaccct cggtgttttg gatttcggaa aggacccgcg
    11641 accagggggc tatctccctt tcaggacagg aattaggggc tcgcatcagg ttcatcaaac
    11701 tcaactgtag cctcgcggcg tacttgtttg catacatcag ctttgtgtat ctgattatca
    11761 gcgtaagctt tcactttggc agcgagccat ccttttgtgc tgtatcgata gactaccgaa
    11821 caagttttcc atatatgtat atatcttgaa cttattgata tcattacaga aaaacaacaa
    11881 ccatgagaac gcaaaaaatg aacagagaaa aaattgaagc cttaatgagc gtaatattaa
    11941 aagaagctga cccaaaaaag tttcctgaga agttatcaga ggacgccatc gtatcttttt
    12001 atcgtgcgaa ctcctcttat ctaaattgtt caactttaag agaatggcta agcggcgaag
    12061 cagagccttc agatgagata cttcagaaga ttatggttca gaacaactgg cgcgcaacaa
    12121 aatggtcaaa tcagttacct taatctgttg aatgtgtgcc gccttaaaac aaaaaggcgg
    12181 cctctcgtta aagtaaatta ccctattttc caccaaggca tgaagcctct gccccggttg
    12241 aatcaataca accaccgttc gcagtagtaa tcttcgtacc cggcgcgatc atcacgtgtt
    12301 taatactgct gccgtcagcg cgagtcgctt cggtgtatgt cgaatgtgtg cagcctgcca
    12361 gcgtgaagaa cgcgacagct aagagaagtt ttttcacgtt ttacctacgc ttctgagata
    12421 gatttctcag cattcaccaa tggaagtgat tgtggaacat ccgtttgacc tgctggccag
    12481 cggtagcctg ttacgcggga acgaggaaaa gctttgatat ttacttcatc tgcctggttg
    12541 ccccccaaaa tcattaaatc cccggcggca tttttcccga caacaaagcc gacgtgacca
    12601 ccgccagacc gagacagaat agcaatgcat ccataggcgg gttccttcag ttcattaccc
    12661 caatcgagat aagaattcgc agactcaaaa cgcgttgagc gaatacccgc acgttccagc
    12721 attgccccgg tgaaagcagc gcaccacggt gtttcgtcat ccttaatgcc gccgcgtttg
    12781 atatctttcc agtactgcac aatttctgca gcatgttgct tccctttcat ttcccgaatg
    12841 ccgaggtttt ttcgagcctc gattagccat ttaggatccg tcattacctg tctcctgtta
    12901 tttttgaaat attccctcgt gcccgccaga cagcaataca gatggtgagg ttgagaaaga
    12961 actctgcggg atcaacctgc gtatatttcc cgtaaaaaat tcgtagggct atccacgcag
    13021 cagacagaat cgcgaggtaa gcagttagtg agatgaaaaa acgatagcgg cctgtttttt
    13081 gaaaaaacat caaccgtagt gccaacatca gacaaataat tgcattggca ttgatgagga
    13141 cagagtgcag ggtcattatt tcatcccctt atctgatttc cataaacgag cttgtgacat
    13201 gatccgcaac agtgatgtaa cgctgattac tgatgcaacc agtgcaccaa tggcgggaga
    13261 gacttttact gttacgggag ggttcaggtg actcagcacc gcgttcagaa taccggtaat
    13321 gatggctgac gctgtctcag cgcaatacac accgccaaca aacgaaatga gtgcgaaaat
    13381 aacctgcttc cagaatcgat gctgcccgga cgacaacaca tagagagctg cgcctgccag
    13441 cgcacagatc atcactgcag gtgttgcctc aggaaataac gcagcaaacg tcacccccgc
    13501 cgagccagca gcaacgccag cggttagagg ttcagacatg atttttcctg attcagaaac
    13561 gacaaaaccc cgccgtggcg aggttcttag aaatttggca acataccaaa ttagcgtcaa
    13621 atatggccta ttttgttcgt ttttgcaagc attatacgca aacgtcgtct aaatggttct
    13681 gataatgatg caaagttgca agaagtgcca cttcatcaag tgctctcacc gcctctttca
    13741 ttcctaccca gtgcagggag tatgtttcgc accaggtgga acgagaaacc gacagcattc
    13801 ctgctagggt agatccggcc atctccttaa aatcctcgcg tttatttgct gctgcgaccg
    13861 cctgcacgga caaccacacc aacgaaagca ggcgcttaac tacttttctc tgagttgacg
    13921 cggggatgtt tcctttatag cgactccaga cagcctcaca aatagctgtt tggtgtttga
    13981 atgccaggtt accaccgtag caataacgca accacgcttg ctgacttgca tccagcgagt
    14041 tcacagaacg ccgccaggat gaatcagcaa attcaaaatc actcatcggc ggcgcggggc
    14101 gacgtctgct gaaactttcc agagcgtaaa ctggcgtaac cagagcatca acggtccggt
    14161 cctccagctg cacacggtga atatgtttgc gtgggtatgc gtttttgtct gccggtgggt
    14221 gctcacacaa tgcttccagt tgccccttag aaccaccaga caaatcccgc aatgcagcgc
    14281 gcagttttat gcgggtatat tccaataaat actcgttact catcagataa actcctcatc
    14341 gcgccagata gccagtgttc tgaaaactcc ctcagcgtgc atcaacaaca atgagtcgtg
    14401 ctcgtaatcc gtttttgtac gtccgtcgat ggcatcgtga cacgcactgc aggcgatagc
    14461 gccctgcata tcgtgaggtt tgatggctgt gccgcacgtt ccggcgagac gataatgagc
    14521 cagtacgctg gtttcaggat tgtgattgca cacaccgggg atacgcacag tacaagcgcg
    14581 actgcgtgca gcttttctca ggtcgatttt tttcatgatg tgtaatccag cagctgcgcg
    14641 gcggcatttt cagcggcctc ctgagaggca aatgtgcgga acaaaatata attccacagc
    14701 acatctaaaa cggctgaata cagttgaccg aactcaagat cgtccatcgc tgcaaacgag
    14761 attgaacgag gtacgcgcat caaactgccg tctggcatct gatgaacgtc ataaaaccca
    14821 gcttccataa tcgcccatgc gcgaaacgcc tcgaacgact tcaccgcact gatactcccc
    14881 gcgcgctttt ccgactcatc gagaagatac tgatcagcca gctctgccat gacatcaccg
    14941 tgtccaccgt aatacgccag taacttaacg tagccgtgaa ccagttcgcg ctccgcagga
    15001 gaaatagccc ctccggacgg atgccagtaa tcaaagccca ggttgagaag tgagaaaaat
    15061 ttacgatgaa acgcgggatt acgcgcctgt ttgaaatctg catacacaac agcacctggt
    15121 ttgactcgtt cagtcaacca gcgttgcgtg tctggtgtcg cgggtattag cgaaccgccg
    15181 gataccctga taaatgaatg ctgcgccatg gttaactcca ggtggcgcgg cagtgttcag
    15241 aaaatgatgc cgggtgttca atccagcacc gatattatac cgtgttttac gcgtcagggg
    15301 gtgtaatagt atatccagct atttctgcca actcgagaaa cgccatcagg ctggcaatcc
    15361 gctcatcatc ggtcagaaca cgcacaccaa cgatgtgtcc gtccttacgc ttgataacag
    15421 cccggcatgt ttccggtagt tcgcctatag attcttgaat attcatgtct tccccccaat
    15481 gccacaacct gtatatttat acagtatttt atggcatttg tcataacgcc aaatacaatg
    15541 agggaataat gaacaattat attcacgtcg tgaattttat taaacggttt tataggggat
    15601 taaaccgttt atggtcatta tttatcactc ctgtaaattg gaattaacca ttttctaatc
    15661 gtcgacgctg caggtgggtc agagtatttc gccacaaaaa taccggatat gtattgcacg
    15721 ccacgcgata atggcgctgt cgggttttca gtaaaaaaac gatcagccag ttcaatggct
    15781 tcgcttttta atctgtgctg tggtcgtcct ccgccctttg ttcccaggta tcgcgccagt
    15841 tgtttgaaac ggtaggcgat gattaacggt cgcatcatca tattgaaaaa atcgcgggca
    15901 tcgttgtcag cgccttgcgc tatcaacagt gcaaaaaaca gatacgggat tatgtacact
    15961 tcaggagata tacgaacccg ttcacaagtt ggtggagcca actcaataag taagtcagcc
    16021 agcggattcc ccttttctcc acgtttgtaa tagcgataca gcggcccgat atcaagttca
    16081 ctcacgtcga tttcaggatg cccggcaacg actgtacgta tctcttctgc cagagcgtca
    16141 aatattgccc cggtccgttc aagatagagc ctccgggtcg aaccgcgatc atccaacgcc
    16201 cgtaacatat ctaaccggct aacgaaagtt tcaggccagt cctcatagaa ggttttcata
    16261 attacctcga ataataaaaa ctgcactatt tgcacaattt attattcgtt ttcacgccgc
    16321 tgagttctat aaaaaagaaa aaacctccga ggcagggttt tgttatggct cgttaaatca
    16381 tcatccaact ttttagactg aacggtgata cttgcaaata tttgaaaata tgggctggaa
    16441 ccctatctta tgatgtgcct ggtaatttat attgttgggt ctttatatca tgaggaatca
    16501 ctatgggtac gttaggcaac cagccgtgca gaagtcgtta tgagttcgat gattttaatt
    16561 tagccaatcg agctgcggct cttactaagt tagcaaaaga aactggtgta tcggtggata
    16621 ccttaatcaa acttgaaatt gctatgcaac tgaatcggct caactccatc cttatcgaaa
    16681 acggtgacaa gatggacgag aatctggcag gtattggcga gatactgcag aagtgagagt
    16741 agcccctctt ctgaggggtt gcacatttaa aatattaagg gcggtcttct tcacgtacac
    16801 agtcacctgg tcgaatcgtt cacgcatact gagtacaaaa aatctcaacg ataagatcag
    16861 gttagacctg tattttccag aaacatatag acgcctaaaa aaacactaaa ctcatgatta
    16921 tcatgtatgt ttttactgga aacattgaac catccacaat acagcgtaaa aagcaagcaa
    16981 ttcacaagat tttaaagtga tttatctgga agactttact actaacagtt aatatcgtac
    17041 cagaagtgca gaatttgcac ttttacagga atgtggtacg gaacatgctg tctatatttc
    17101 aaccaataat cgtaatgcag taactggagg agctatggct accaacccaa gagagcacgc
    17161 ccggcgagtg ctggacacgt tctggtgcgg gcgtggtttc cctgttgatc cagcgaaaat
    17221 tgctcacgac atgggattag atgtttttat taccgatctt cctgggaaag tttctggcgc
    17281 actgattaaa caaaaagatc aagatccagc gatctttcta aacagtgatg acaacaaagt
    17341 tcgtcaacgt ttttcatgtg ggcatgagtt ggggcattac atcgcccggc aagcatcgca
    17401 tagcgatgaa tatgagtacg ttgatttacg tggcgaaacc gcaagcgccg gaacgcatcc
    17461 tgatgaaatt tttgcaaaca agttcgcagc agaacttctg atgccggtag atgaggtcag
    17521 aaaactgcac gctgacggtc aaccaagtta tataatggcg cattattttg gcgtatctga
    17581 cgatgcgatg acttatcgtt taaagaactt gaggttgggg taacgcttta aaatgccatc
    17641 ggatgacgat tttaacagcc gtttcaaaca ggaagtagca cgcagactga aggattccct
    17701 caacctacac cgtcagttag cagaagaggt tgagccggag gacgatgaaa ctccggttca
    17761 gtctcctgac gaagcttcac ctcagccaaa ggtttcgcac gcccccaaaa aaacggctgt
    17821 ccgtattcgc ggaatagcca gaaaaagagc tgccgaagct cgcaaaatgg ctgtagctgc
    17881 gtctagcaaa agaattccaa ctgctacacc ctctcaacaa gtcacccgta ctgagagtaa
    17941 aatcttgacc gagttcacat ctgtgaaaca ggtgaattca cttgataccg agcatgagcg
    18001 caaacaactc aacaacaaag attttgaagc agaaatcgct ctaaaaaaac gatacgggaa
    18061 atggttcctg attatccttg ctttccaact tctgataatg aatggtgtat ttattgccga
    18121 cggcgcaggt aagctggaat ttgaggattt aacactacag ttgtacatgg gcggtacttt
    18181 gacagaggta ttcggcctcg tgctggtcgt gacaaaatac ctttttaaac gcaagtaact
    18241 ctctcggtcc aacagtcttt cgaaggccct gatcaaaggg ccttgataaa ttagcagtgc
    18301 gttgaaaaca gatagttatt cccatcagtt ccaacgtaat acacatcaaa aaaatcacca
    18361 ttcttttgct ctaacgtcaa cacctaccct taaaaaaata attctccaag aatgagaaag
    18421 ttattgtgtg tattagataa gatatgagct gacacatcta tggcgtggag ccaaatttaa
    18481 tatgacagtc cgctctgtgc cataagcgga ctgtcaataa a
//
LOCUS       DAPQZD010000089        18513 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPQZD010000089 DAPQZD010000000
VERSION     DAPQZD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.089"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            40..1179
                     /gene="arnB"
                     /locus_tag="RO454_002989"
     CDS             40..1179
                     /gene="arnB"
                     /locus_tag="RO454_002989"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDY0934790.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1183..2151
                     /gene="arnC"
                     /locus_tag="RO454_002990"
     CDS             1183..2151
                     /gene="arnC"
                     /locus_tag="RO454_002990"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDY0934791.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2151..4133
                     /gene="arnA"
                     /locus_tag="RO454_002991"
     CDS             2151..4133
                     /gene="arnA"
                     /locus_tag="RO454_002991"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDY0934792.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4130..5020
                     /locus_tag="RO454_002992"
     CDS             4130..5020
                     /locus_tag="RO454_002992"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDY0934793.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5020..6672
                     /gene="arnT"
                     /locus_tag="RO454_002993"
     CDS             5020..6672
                     /gene="arnT"
                     /locus_tag="RO454_002993"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDY0934794.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            6669..7004
                     /gene="arnE"
                     /locus_tag="RO454_002994"
     CDS             6669..7004
                     /gene="arnE"
                     /locus_tag="RO454_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDY0934795.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7004..7390
                     /gene="arnF"
                     /locus_tag="RO454_002995"
     CDS             7004..7390
                     /gene="arnF"
                     /locus_tag="RO454_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDY0934796.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7384..7650)
                     /gene="pmrD"
                     /locus_tag="RO454_002996"
     CDS             complement(7384..7650)
                     /gene="pmrD"
                     /locus_tag="RO454_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDY0934797.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7760..9115)
                     /gene="menE"
                     /locus_tag="RO454_002997"
     CDS             complement(7760..9115)
                     /gene="menE"
                     /locus_tag="RO454_002997"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDY0934798.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9112..10074)
                     /gene="menC"
                     /locus_tag="RO454_002998"
     CDS             complement(9112..10074)
                     /gene="menC"
                     /locus_tag="RO454_002998"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDY0934799.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10074..10931)
                     /gene="menB"
                     /locus_tag="RO454_002999"
     CDS             complement(10074..10931)
                     /gene="menB"
                     /locus_tag="RO454_002999"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDY0934800.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10946..11704)
                     /gene="menH"
                     /locus_tag="RO454_003000"
     CDS             complement(10946..11704)
                     /gene="menH"
                     /locus_tag="RO454_003000"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDY0934801.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11701..13371)
                     /gene="menD"
                     /locus_tag="RO454_003001"
     CDS             complement(11701..13371)
                     /gene="menD"
                     /locus_tag="RO454_003001"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDY0934802.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERGRFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13460..14755)
                     /gene="menF"
                     /locus_tag="RO454_003002"
     CDS             complement(13460..14755)
                     /gene="menF"
                     /locus_tag="RO454_003002"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDY0934803.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14834..15139)
                     /gene="elaB"
                     /locus_tag="RO454_003003"
     CDS             complement(14834..15139)
                     /gene="elaB"
                     /locus_tag="RO454_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDY0934804.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15194..15655)
                     /locus_tag="RO454_003004"
     CDS             complement(15194..15655)
                     /locus_tag="RO454_003004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0934805.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15720..16637
                     /gene="rbn"
                     /locus_tag="RO454_003005"
     CDS             15720..16637
                     /gene="rbn"
                     /locus_tag="RO454_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDY0934806.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16758..18500)
                     /locus_tag="RO454_003006"
     CDS             complement(16758..18500)
                     /locus_tag="RO454_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDY0934807.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPATARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKH
                     ITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     ASAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
BASE COUNT         4266 a         4983 c         4686 g         4578 t
ORIGIN      
        1 atataagtgg acaggtattc aatgacggat ggaaaaacaa tgtcaggatt tttgcctttt
       61 tcgcgaccag caatgggcgt agaggaactc gctgcagtta aagaggttct cgaatccggt
      121 tggatcacaa ccggtccgaa gaatcaggcg cttgaacaag ctttttgcca gttgacggga
      181 aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg
      241 ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc
      301 aatatgattt ccttgctggg tgcaacgccg gtaatggtgg atgtcgaccg cgatacgctg
      361 atggtcacgc ctgaagcgat cgaagcagcc attacaccac gcactaaagc catcattccg
      421 gtgcattatg ccggtgcgcc agcagatatt gacgccattc gcgccattgg cgaacgttac
      481 ggcatcgcag ttatcgaaga tgctgcccat gccgtaggta cgtattacaa agggcgacat
      541 attggcgcaa aaggtaccgc tattttttcg tttcacgcca tcaaaaatat tacctgtgcg
      601 gaaggtggcc tgattgttac tgataatgaa aatcttgccc gccagctgcg gatgctgaaa
      661 tttcacggtc tgggtgtcga tgcctatgac agacatacct ggggccgtgc accgcaggct
      721 gaagtcttaa cgccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg
      781 acacagttag tcaaattaga gcacctcaac acccggcggc gcgaaattgc ccagcaatat
      841 cagcaagcac tggcaactct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt
      901 cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat
      961 gcgttgatgg aagcgttaaa agaaagaggc attggtaccg ggttacattt ccgcgctgct
     1021 cacacacaaa aatattatcg cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg
     1081 aatagcgaac gcatctgttc attgccgctg ttcccggata tgaccaccgc cgatgccgac
     1141 cgcgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct
     1201 gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta
     1261 atcaggcgca ccaccgcagc ctgtgaatca ctggggaaag agtatgaaat cctgctgatt
     1321 gatgacggca gtagcgataa ttccgcgcat gtgctggtcg aagcctcaca agcggagggc
     1381 agccatattg tgtctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg
     1441 ggattcagtc acgtcaccgg cgacttaatt attaccctcg atgccgatct ccagaatccg
     1501 ccagaggaga tcccccgtct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact
     1561 gtacgccaga accgccagga cagctggttt cgtaaaaccg cttcgaagat gattaaccgg
     1621 cttattcagc gcaccaccgg caaagcgatg ggtgactatg gttgtatgct gcgcgcctat
     1681 cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt
     1741 ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcaccatgc cgagcgtgag
     1801 tttggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctggtgacc
     1861 tgccttacca caacgccgct acgtatgttg agtttgctcg gcagcattat tgcgattggc
     1921 ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg
     1981 gcagaaggcg tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc
     2041 ggcatgggat tgctcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc
     2101 cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg
     2161 tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg tctgccggtt
     2221 acgaaattag cgccattttt acccatactg ataaccccgg tgaaaaagcc ttttatggtt
     2281 cggttgcccg actggcagcg gaaagaggca ttccagttta tgcgccggat aacgttaatc
     2341 atccgctgtg ggtggaacgc attgcccaac tatcaccaga ggtgattttc tctttttatt
     2401 atcgccatct tatttgcgat gagattttgc agctcgctcc gagaggtgca tttaatctgc
     2461 atggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg
     2521 gtgaaacgga aactggcgtt acattgcacc gaatggtgaa acgtgccgat gccggggcca
     2581 ttgtggcaca actgcgcgtt gccattgcgc cagacgatat cgccattacg ctgcatcata
     2641 aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta
     2701 atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg
     2761 atgacagttt cctcgaatgg cataagccgg catccgtact gcacaacatg gtgcgtgccg
     2821 ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt
     2881 cgtcgcgtgt tcatcctcat gccagcaaag cactgccggg gagcgtgatt tctgttgcac
     2941 cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt cactggacag gcgggcgacg
     3001 gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct
     3061 tgaatagcca gcctgcctgt gccgcccgac gccgtacccg ggtactcatc ctcggggtga
     3121 atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt
     3181 acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact
     3241 ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg
     3301 atgtcgtgtt gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc
     3361 gcgtatttga actcgatttc gaagagaatc tgcgcattat ccgctactgc gtgaagtacc
     3421 gtaagcgaat catcttcccg tcgacttcag aagtttatgg gatgtgtagc gataaatact
     3481 tcgatgagga tcattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt
     3541 cggtgtctaa acaattactt gatcgggtga tctgggccta tggcgaaaaa gaaggtttac
     3601 agttcaccct cttccgcccg tttaactgga tggggccacg actggataac cttaatgcag
     3661 cgcgaattgg cagctcccgc gctattacgc aactcattct caatctggta gaaggttcac
     3721 cgattaagct gattgatggc ggaaaacaaa aacgctgctt tactgatatt cgggatggta
     3781 tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgatggc gaaattatca
     3841 acattggcaa tcctgagaac gaagcgagca ttgaagaact gggtgagatg ctgctggcga
     3901 gcttcgaaaa acatccgctg cgccattact tcccaccgtt tgcgggcttt cgtgttgtcg
     3961 aaagtagcag ctactatggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc
     4021 gcaatgcccg ccgctgcctg gactgggaac cgaaaattga tatgcaggaa accatcgacg
     4081 aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt
     4141 cggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct
     4201 ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa
     4261 tatgggccgc catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc
     4321 aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa
     4381 agagattggt catgccaatg ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg
     4441 cctgcacgcc tgggatcacc atgcctggca agcccatagc ggtaattggg atcggcaaac
     4501 aatgatcgac gatattgcac gcggccttcg caccctggaa gagattatcg gtcaaccggt
     4561 aacctgttct gccgctgctg gctggcgtgc tgaccaacag gtgatcgaag caaaagaagc
     4621 gttccatttg cgctacaaca gcgattgtcg tggggcgatg ccgtttcgtc cattgctcga
     4681 atcaggaacc cccggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat
     4741 tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca tccagcgaga
     4801 taaaggcacg ccggtttata ccattcatgc cgaagttgaa ggctgcgctt atcagcataa
     4861 ttttgtggat ctcctgaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga
     4921 actgttatca gggacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga
     4981 aggctggctg ggttgccaac aaatggcggg tagtcactga tgaaatcggt acgttacctt
     5041 atcggcctct tcgcatttat tgcctgctat tacctgttac cgatcagcac gcgtctgctc
     5101 tggcaaccag atgaaacgcg ttatgcggaa atcagtcggg aaatgctggc atccggcgac
     5161 tggattgttc cccatctgtt agggctacgt tatttcgaaa aacccattgc cggatactgg
     5221 attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt
     5281 atctttgcga ccctgttaac tgccgcgctg atgacctggt ttactctgcg cttatggcgc
     5341 gataaacgtc tggctttact cgccacagta atttatctct cattgtttat tgtctatgcc
     5401 atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc
     5461 agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg
     5521 ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg
     5581 gtattaagcg tgctgccatg ggtagcaacg caaaaacgct ggaaagatct ctttatttac
     5641 ggctggctgg cggttatcag ttgcgtactg acggttctcc cctggggact ggcgatagcg
     5701 cagcgggagc ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca
     5761 ctggatgatg cccaacatag agcgccgttc tggtactact tgccggtcat cattgccggt
     5821 agcctgccgt ggctgggatt actccccggt gcactgtacg ctggctggaa aaaccgcaag
     5881 cattccgcaa ccgtttattt gttgagctgg acgataatgc cgctgctgtt tttctccgtc
     5941 gctaaaggca aattgcccac ctatattctt tcctgctttg cacctctggc aatgctgatg
     6001 gcgcattacg ctttgctggc agcaaaaaat aatcctctgg cgttgcggat taatggctgg
     6061 attaacatcg cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga
     6121 ccaatgaaca caccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtct
     6181 attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg
     6241 ccttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac
     6301 agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag
     6361 ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagcctg
     6421 caacgcgatg acatcatcat gtatcgccag acaggtgagt tgaaatatgg ccttaattat
     6481 ccggatgcaa aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat
     6541 cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaatagt
     6601 ctcgccattc cgcccgctga tgccatcgat cgtcagggac gtctggtact cattcagtat
     6661 cggcccaaat gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt
     6721 tgtgtcagaa acaggcaacc tgctttgcgg cggtaaacaa acggcgcaaa catatcgtgc
     6781 tgtggctggg actggcgctg gcttgtcttg gccttgccat ggtgctctgg ctgctggtct
     6841 tgcagaacgt accggtaggc attgcctacc cgatgttaag tctgaatttt gtctgggtaa
     6901 cgctggctgc agtaaaactg tggcacgagc cggtatcgct gcgtcactgg tgtggggtgg
     6961 cgttcattat tggcggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatgtgggg
     7021 attattcagc gtcattatcg cctccgctgc gcaattaagc ttgggttttg cggcgagtca
     7081 tctgccgcca atgacgcact tatgggattt tatcgcggcg ctgttagctt ttggcctgga
     7141 tgcccggata ttgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa
     7201 aacactacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct
     7261 ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact
     7321 actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca caacaaaaca
     7381 acgttactga gttttccctg ccgctttaca ctgccgttcc cactcatcgg ggctataaca
     7441 gcttgcagaa agaactttca ccgtgtgcag cttttcacga ttaatacagt aaagcgcatt
     7501 ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat
     7561 catttttatc gcgccgccag catcgcagag cataagtaca tgtctgttat tttgtttatt
     7621 gcagcacgat tttttgacca gccattccat tgcattatcc tgtttgataa gagttttcac
     7681 atcaatttac agattaacag ataaacctat tatcaacggc aatggaatgg caaaattgcg
     7741 aagtgaaact aatgatttct taatcctggc gttgcaccca ctcttttaac gcctgacgtg
     7801 aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc accggttgtt
     7861 gaaaacgggc cagcttatct ttcacccatt cgccaagatc aacggtttgc tggtcatact
     7921 ccaccaccgc taccggtcga tgaccaaact ccttgtcagc aacgggaacg ataaacacct
     7981 gcaaaaccgc aggatgtgca gcaataacgc gctcgacttc ttctggctga atgccttcgc
     8041 caccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
     8101 caccgcgatc gcgcgtggcg taccagcctt cgtcattaac cagtggaacc cgttgcccgt
     8161 tacgccagta accttctgcc atgctggcag cccgcagcca cacttcgtcg tttacgattt
     8221 tcacttcccg acccggcagc ggcgaaccga cgtctgccag accgtcggct tctttcgcac
     8281 acaccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaccgaatc ccctgctcgc
     8341 gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaaaacc gctttcaggg
     8401 aaacggaact acggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgcg
     8461 tacagcctgc cagcatttgc tccaatggct gtttatcacg taccgtcatc cgcgcaccag
     8521 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
     8581 accagtcatc gtgatcgcca aacggaatca gcgataacac cccttcggcg ctggcaagat
     8641 gagcctgata agtatgaaca gcggctttcg gcaagccagt ggaaccagag gttaacgtca
     8701 ttgagcacag acgcgtcggc tgccacgcca cggcatgtac gccttcaacc tgctgaatat
     8761 gcagcgacgt taacgccgga aacgtgtttt ccccttccgg caccagcgca aattgcagcg
     8821 taagattggg cagcaattcc tcaagcagcg gttgcggcag ctgagggttc acgggcaaca
     8881 cccgcgcccc gcattgcagt aacgccagcc aggccagcag cgtttgcggc gtattccacg
     8941 cccgcaacat cacgccgctg cccgcaacca caccctgcgc cgcaaagctg gaggctaatt
     9001 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
     9061 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacag
     9121 ccgctccagt gcatcaacgt ccactaacgg cagtggactc cccggccagc gacgaacctg
     9181 ctgcacctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg ttaaccaggc
     9241 agcaatccgc gccagttgca ttagacctaa gctcgactca atggaagaac tgatcaccgc
     9301 cgtcagcccc agcgcatgcg ccgccttcac ctgctcgcgt actttctcca ggctgcccgt
     9361 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gctacaaagg caaaatccgg
     9421 ctcgcgcaag ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgcacgcga
     9481 atcatcgcgg gttttgcacg gctcttcgag aaatgcgatg cggtggcgat aatccgggtt
     9541 aacgtatttg gcaaactgct ggcctttcag cggtgtccag gcgcgattcg cgtcaagacg
     9601 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcacggc
     9661 ttcatacaat cccactttga ccttcgccac tttctcgcca ggcatatcgg caagtttgag
     9721 gatcagatcg tcaggatcgc cattacacag cggtgccgca cggtaattgg ccgcctgcgg
     9781 gagcgtatct gccagctccg ccaatgcaca acttacgcca aaggcaacgg aaggcatctg
     9841 cggtaattcg caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc
     9901 atcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg
     9961 ctcaccctca cgcaggcaaa catacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    10021 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    10081 ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcatt gcgaccttcc
    10141 tgaccttctt ccgtcatgta gaacagcatg gtagcgttgc ccgccagctc ctgcagcccc
    10201 gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc catcgggctg
    10261 ttttgcagca tttcacggca ccaacggacg gtttcttttt ccagatccgc cagcggtacc
    10321 acggtgttta caaggcccat atccagcgcc tgtttcgcgt cgtactgacg gcacaagaac
    10381 cagatttcac gcgctttttt ctgtccgacg atgcgagcca tgtaggaagc gccccagccg
    10441 ccgtcgaagg aaccgacttt cgggccagtc tggccgaaga tggcattatc agccgcgata
    10501 gtcaggtcgc acatcatgtg cagaacgtga ccgccgccga tggagtagcc agccaccatc
    10561 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    10621 tgtacgccgg aatcatcttt atagccgccg taatcgccac gcactttctg gtcaccaccg
    10681 gagcagaacg ctttatcgcc tgcaccagtc agaatgatca cgccgatgtt gtcgtcataa
    10741 cgcgcatccg ccagcgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    10801 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    10861 tcctcaaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca
    10921 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    10981 cgccagcggg attttcccgg tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    11041 cagccagttc cgccgccaga gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    11101 acgcaaatgt gcgggcgcta aggttagcac gtaaatcggg ctggacggcg agagaagtcg
    11161 cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc accagctccc
    11221 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccaatcg gcaaataccg
    11281 ccgttaacgg ttctgtacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    11341 cgcgttgttc agcattttgc aacccaggat gcccgccttc gacaacaact ccacaaagcc
    11401 ccgccagctc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag taccccacca
    11461 gccagaagtt aaggatgttg taactaacca gcgttttaca cagtaagtcg gtgacatcat
    11521 caaatccatc gacgctgatc gtcgctgaac caccgtgacc tgggagatca acatacaacc
    11581 gcgagtagtc ggcaaacgcc tcgcccactt cttgccattc atggcaatcg ccggaaaaac
    11641 cgtgcaaaaa caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga
    11701 tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt
    11761 aaccaccatt tcaatcaccg tggtggtcgg cgtacgccag gcatcgacta gtgtcgtttc
    11821 aagttcctgc cagttttgcg gacgatgata tttcagctcg aacatcgcgg cggcgtgctc
    11881 aaaatggacg ttttgcggca tcagatagaa acgcccgcgc tcgcttttcg gcgttggcaa
    11941 cagcgaaaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gtgcggaaac
    12001 ctggcgcaat aacgccagcg cgttgagatc gtaaagtgcg gagagatcgc ccacaatcgc
    12061 cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat
    12121 accgctggca ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    12181 aatcagacgc accaccaggc tgttaccgac aaacaattgc ccctgttcag gcaaatagtc
    12241 gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacgggcaa taaccgcctg
    12301 cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    12361 atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat ggtgtgccgg
    12421 atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac agcttgcctg
    12481 ccattggagg agtcgtttcc ccgtcaggct gcttcccaat tgcaccacta tttgtgcctg
    12541 ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg
    12601 tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagtgc
    12661 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacaccgc gcttttgtcg
    12721 ccagaagaac cagtcgcgct gtttttcact ttcccgacga ggcgcttcac gcagccacgg
    12781 tttgtcgtcc tgccaccagt cgcccagccg ctgttgccag ctaatccccg tgtcgtccat
    12841 ttcgccatac agcggttcag caaacgggca gttgatatgg actccaccag catgaagcgt
    12901 accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    12961 cggcagtgaa atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg
    13021 attcgcgccg cagtcaatca gctccggcgg gcgatcggcg gttaacagga tcagtttttc
    13081 tccggttaac ccagcttcaa tcagcgccgg atagagattt gctaccgccg tgccggaggt
    13141 cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag
    13201 tccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    13261 taacggtgta gaacgcgagc caggggcgat acagatgtgt ctgacgccgt gacgcgttaa
    13321 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    13381 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    13441 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    13501 tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgcct gcgccagcat
    13561 atagccgcac gatattgccg ctgatttttg ctgagcgcag ggaaacgcag aattcacttt
    13621 gctgtaatga aagatagccc gcagaaccgg cgtaccattc tcgagtgaac ggttcgtgac
    13681 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    13741 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    13801 gatgctgtac tttacgtaga cgcagtacct gcggcggtaa aacatccagc gtccgggtat
    13861 cggcctgcaa tcgttggcag atatcttcca ccaccaacat gttctcgcgc tggtttttat
    13921 catccgccat cagccactca cctaactgct gcgcctgctt atcatcagga tgatttgcta
    13981 ccgttcccgc cagcgcttcg gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    14041 gtgaagaacc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattca
    14101 gattgagccg acgactggca gccatcatcg ctgccgcatt gaccggactt gcgaaatgca
    14161 ggtcagttgc ccgagcaagc acaactttgt cgagctcgcc ttcggcgatg gttttcgttg
    14221 ccagttcgat taatagcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg
    14281 ttaaatgtaa tccaggcagg ggcttgatac tcaccagtgt ggcgataaat tcttttgcct
    14341 tgattgcatc gtgctgaagg gagctttcgc tgaatagcgt caaccgcagc gtggctttac
    14401 cgccacaacg tcgccattcc aggcgtggta aaagtaaata gccctgcgac gggtcaaacg
    14461 cattcaaccc ccagatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    14521 cctggtccaa cgacgtaaaa cgggtaatcg cacccaggac ggcagcttct tcatcaccat
    14581 tacgttgttg ccagtagaat tgcggataca cctgctgact ggccagccag ctcaaggcat
    14641 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    14701 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    14761 cctctccctg ttaaaagcct cacattatac ggggtacgac aaaaaaaatg cagtaccccg
    14821 gtgtagggag gttttaacgg cgtgccagca acagtcctag aaccagccca acggccgcac
    14881 ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga taaactgcct
    14941 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    15001 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    15061 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatca atacgtgtat
    15121 caccaaactg attagacatt ctcgttctcc atttgtgtaa aacctgtcac taactataga
    15181 caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat
    15241 cttcttcata aatatcggtc acagggatga aaccaaattg cccataaaag ttttgtaaat
    15301 gcgcctgcgc ccccagataa atcggcttct ccggccagcg gcgagtacac gattccagcg
    15361 ccttactcat caattgctga ccgatttttt caccgcgtaa ggcttcgctg acaatcaccc
    15421 gaccaatcac aacgggttgc agttcgtcgt cacttttcag aatcctcgca tacgccacca
    15481 gtgtcccgtt atgccaaccg agaatgtggc gattttctcc ctccagatcg tcaccatcga
    15541 tatcctggta aggacagttt tgttccacta caaataccgc acaacgcagt tgtaataagg
    15601 cataaagctg agaaacggat aaatcagaat gatgcagatc ttgccagtcg atcatggaag
    15661 gctcctttgt gctgtttgct ttatactagc cggaatattt tcagcagcgt gatgaactca
    15721 tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgtacgcgt aatgtcacgg
    15781 caatattgct taacctgcaa catccgaccc agagcggact ttggttattt gactgcggtg
    15841 aaggcaccca gcatcagcta ctgcatactg cctttaaccc tggaaaactg gacaagattt
    15901 ttatcagtca ccttcatggc gatcatctct ttggtttacc cggcttgctg tgcagtcgtt
    15961 ctatgtcagg cattatccaa cccttaacga tttatggtcc gcacggtatc cgtgaatttg
    16021 tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa
    16081 ttagcactgg cgaaattttc gatgatggcc tgcgcaaagt gaccgcttat ccgatggaac
    16141 acccactgga atgttatggt tatcgtattg aagaacatga taaaccaggt gcattaaatg
    16201 cccaggcatt aaaagctgct ggcgtgccgc ctggcccgct gtttcaggaa ttaaaagcgg
    16261 gcaaaacaat catgctggat gatggaaggc agattaatgg cgcagattac ttagccgctc
    16321 cagtgccggg taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcgctgg
    16381 agcttgctaa aggcgtcgac gtaatggttc acgaagcgac tctggatatg gcgatggagg
    16441 ccaaagccaa cagtcgcggc catagttcta cacgccaggc ggcggcacta gcccgtgagg
    16501 ctggagtcgg taagctaatc attacccacg tcagctcacg ctatgatgac aaaggttgtc
    16561 agcacctgtt acgtgaatgt cggtcaattt tcccggcgac tgaactggcg aatgatttcg
    16621 cggtgtttag catttaagtc gaaagcggtg gttagttagc agattaattt aacgatacca
    16681 acacatttat ttgacatttt ttcatctgtt tgccggatac gtttgatgtc gatatcaggc
    16741 aaacagaatt atcattttta gttttgtgaa ttgtccagat ctttcgccag tccaatcagg
    16801 cgaataaatt ccgctctgta accctgtgga tcttcccctt ttgccttctg agcccactgt
    16861 ttgatctgct gccaggaggt attgttcagg tattcagaac cgcgtaactt ttgcccatat
    16921 gcagccactg ctgcgcgaaa gcgcatatct tctgaaggtg cctttattgc agatgccaac
    16981 gggaattcaa ctaactgact ttcttttccc tgtggggatt tccaacgaat ctttaaccag
    17041 gcaagttctt ttgttttgtc cgatttcgct gatttgttat ccggggcata gcgtaactta
    17101 tcaattgatg ctttttgccc tttcagcgtt aattcaaata acaatgttat atgtttacct
    17161 gcgccaatat cacccgcatc aacgttgtcg ttattaaaat cctctgcctt aagttggcgc
    17221 ttttcataac ctatctgacg gtattccgtt acccacgcgg gattaaactc aatttgcgct
    17281 ttgacatctt ttgctacggt aatcaacgtc tgccgcattt cactgttcaa cactttctgc
    17341 gcctctgaga gggtatcaat gtagctatag ttgccgttac caacatcggc aattctcacc
    17401 atcattgcct cgttataatt gctatccccc acgccaagcg tcgacagagt aacaccggac
    17461 tcacgctgtt ttttgaccat tgattcaatc gattttggat cgtcaatgcc aacgttaaag
    17521 tcaccgtcag tggccaataa aatgcgattg ataccacctt taataaaccc cttcgctgcc
    17581 tgctgataag ccatctccag cccggcaccg ccattggtac tgccttctgc atccagcgaa
    17641 tcaattgcag cattaatttc cgccttgtga ctaccggaga tagaaggcaa cgcaatacga
    17701 gagtcgccag cgtaggtcac gatggcaatg ttatcctgct cacgaagttc tttgaccaat
    17761 aatttcaacg aagactggat aagtggcaaa cgttcatcag aaatcattga accagacgtg
    17821 tcgataagaa agaccagatt agaagcgggt aactcttcac ttttgcgatc tttcgccagg
    17881 atatcaactt tcagcaatgt tcgctgttca ttccagggtg caggtgccaa ttcgtagcgc
    17941 atagcgaaag gtattggctt agaggccgga atagattgtt tgtctttgat atcccagtca
    18001 gacgggaaat aattgactat ctcctccacc cgcacagcgt ctggcggagg taacagccct
    18061 tgattaagga aacgccgcac attcgcataa ctgccggtat cgacgtcaag gctaaacgtc
    18121 gccaacgggt tttgcgctac ctgcttaacc ggattatcat cgaactgctg gtagcgagcg
    18181 gttgctggat ttgcgatttg ggaggctgct ttttctctgg ctgcatgttg atattttgga
    18241 gccgcctgca atcgccccag caaagcctgt ttgtctgaat attgttgcac ttcttgctgg
    18301 gctaaggctt tcgcgtctgc agcggctttg gcggcgcttt gctcagccac ttttgttgcc
    18361 gcttgttgcg cggcaagcac ttgctggtca gtgggtgtag tagattgttg ttgctgactt
    18421 ttcttatcct caggttccgg cccacatcct gacaaaatca aactacttat aagcaacatg
    18481 attatatttt tatttcgcat ataaaatcct ttt
//
LOCUS       DAPQZD010000090        18368 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPQZD010000090 DAPQZD010000000
VERSION     DAPQZD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.090"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            22..96
                     /gene="tnaC"
                     /locus_tag="RO454_003007"
     CDS             22..96
                     /gene="tnaC"
                     /locus_tag="RO454_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDY0934808.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            317..1732
                     /gene="tnaA"
                     /locus_tag="RO454_003008"
     CDS             317..1732
                     /gene="tnaA"
                     /locus_tag="RO454_003008"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDY0934809.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1824..3071
                     /gene="tnaB"
                     /locus_tag="RO454_003009"
     CDS             1824..3071
                     /gene="tnaB"
                     /locus_tag="RO454_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDY0934810.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3203..4378
                     /gene="mdtL"
                     /locus_tag="RO454_003010"
     CDS             3203..4378
                     /gene="mdtL"
                     /locus_tag="RO454_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDY0934811.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            4353..5312
                     /gene="yidZ"
                     /locus_tag="RO454_003011"
     CDS             4353..5312
                     /gene="yidZ"
                     /locus_tag="RO454_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDY0934812.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5469..6218
                     /locus_tag="RO454_003012"
     CDS             5469..6218
                     /locus_tag="RO454_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDY0934813.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6240..6806
                     /gene="yieF"
                     /locus_tag="RO454_003013"
                     /gene_synonym="chrR"
     CDS             6240..6806
                     /gene="yieF"
                     /locus_tag="RO454_003013"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDY0934814.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6859..8196)
                     /gene="adeP"
                     /locus_tag="RO454_003014"
     CDS             complement(6859..8196)
                     /gene="adeP"
                     /locus_tag="RO454_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDY0934815.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8363..9028
                     /gene="yieH"
                     /locus_tag="RO454_003015"
     CDS             8363..9028
                     /gene="yieH"
                     /locus_tag="RO454_003015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDY0934816.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9095..9562
                     /locus_tag="RO454_003016"
     CDS             9095..9562
                     /locus_tag="RO454_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934817.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9611..10198
                     /locus_tag="RO454_003017"
     CDS             9611..10198
                     /locus_tag="RO454_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDY0934818.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10259..10981)
                     /locus_tag="RO454_003018"
     CDS             complement(10259..10981)
                     /locus_tag="RO454_003018"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDY0934819.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10996..12165)
                     /locus_tag="RO454_003019"
     CDS             complement(10996..12165)
                     /locus_tag="RO454_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDY0934820.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12192..13808)
                     /gene="bglH"
                     /locus_tag="RO454_003020"
     CDS             complement(12192..13808)
                     /gene="bglH"
                     /locus_tag="RO454_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDY0934821.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13894..15288)
                     /gene="bglB"
                     /locus_tag="RO454_003021"
     CDS             complement(13894..15288)
                     /gene="bglB"
                     /locus_tag="RO454_003021"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDY0934822.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15307..17181)
                     /gene="bglF"
                     /locus_tag="RO454_003022"
     CDS             complement(15307..17181)
                     /gene="bglF"
                     /locus_tag="RO454_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDY0934823.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(17314..18150)
                     /gene="bglG"
                     /locus_tag="RO454_003023"
                     /pseudo
     CDS             complement(17314..18150)
                     /gene="bglG"
                     /locus_tag="RO454_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
BASE COUNT         4671 a         4551 c         4299 g         4847 t
ORIGIN      
        1 tctttcgagg ataagtgcat tatgaatatc ttacatatat gtgtgacctc aaaatggttt
       61 aatattgaca acaagattgt cgatcaccgc ccttgatttg cccttctgta gccatcacca
      121 gagccaaacc gattagattc aatgtgatcg attagtttgc tatatcttaa ttttgccttt
      181 tacaaaggcc atctctcgtt tatttacttg ttttagtaaa tgatggtgct tgcatatata
      241 tctggcgaat taatggatat tgcaaatgta atattcacag ggatcactgt aattaaaata
      301 aatgaaggat tatgtaatgg aaaactttaa acatctccct gaaccgttcc gcattcgtgt
      361 tattgagcca gtaaaacgta ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg
      421 tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc gatttactga ccgacagcgg
      481 caccggggcg gtgacgcaga gtatgcaggc cgcgatgatg cgtggcgacg aagcctacag
      541 cggcagccgc agctactacg cgttagccga gtcagtgaaa aatatctttg gttatcaata
      601 cactattccg actcaccagg gccgtggtgc agagcaaatc tatattccgg tactgattaa
      661 aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg gtggcgttct ctaactattt
      721 ctttgatacc acgcagggcc atagccagat taacggctgt accgtgcgta acgtctatat
      781 caaagaagcc ttcgatactg gcgtgcgtta cgactttaaa ggcaactttg accttgaggg
      841 attagaacgc ggtattgaag aagttggccc gaataacgtg ccgtatattg ttgcaaccat
      901 caccagtaac tccgcaggtg gtcagccggt ttcactggca aacttaaaag cgatgtacag
      961 catcgcgaag aaatacgata ttccggtggt catggactcc gcacgctttg ccgaaaacgc
     1021 ctatttcatc aagcagcgtg aagcagaata caaagactgg accatcgagc agatcacccg
     1081 cgaaacctac aaatatgccg atatgctggc gatgtccgcc aagaaagatg cgatggtgcc
     1141 gatgggcggc ctgctgtgca tgaaagacaa cagcttcttt gatgtgtata ccgaatgcag
     1201 aaccctttgc gtggtgcagg aaggcttccc gacatatggc ggcctggaag gcggcgctat
     1261 ggagcgtctg gcggtaggtc tgtatgacgg catgaatctg gactggctgg cttatcgtat
     1321 cgcgcaggtg cagtatctgg tcgatggtct ggaagagatt ggcgttgtct gtcagcaggc
     1381 gggcggtcac gcggcattcg ttgatgcagg taaactgttg ccgcatatcc cggcagatca
     1441 gttcccggca caggcgctgg cctgcgagct gtataaagtc gccggtatcc gtgcggtaga
     1501 aattggctct ttcctgttag gccgcgatcc gaaaaccggt aaacaactgc catgcccggc
     1561 tgaactgctg cgtttaacca ttccgcgcgc aacatatact caaacacata tggacttcat
     1621 tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat attaaagggt taacctttac
     1681 ctacgaaccg aaagtattgc gtcacttcac cgcaaaactg aaagaagttt aattaaaaca
     1741 ctcagagtgg ctataaggat gttagccact ctattaccct acatcctcaa taacaaaaat
     1801 agccttcctc taaaggtggc atcatgactg atcaatcaga aaaaaaacac tctgcatttt
     1861 ggggtgttat ggttatagcc ggtacagtaa ttggcggtgg gatgtttgct ttacctgttg
     1921 accttgccgg agcctggttt ttttggggcg cttttattct tattatcgcc tggttttcaa
     1981 tgttacattc agggctatta ttattagaag caaacttaaa ttatcccgtc ggttccagtt
     2041 ttaacaccat caccaaagat ttaatcggta acacctggaa cataatcagc ggtattaccg
     2101 ttgccttcgt tctctatatc ctcacttatg cctatatctc tgctaatggt gcgatcatta
     2161 gtgaaacgat atcaatgaat ttgggctatc acgctaatcc acgtattgtc gggatctgca
     2221 ctgccatttt cgttgccagc gtattgtgga taagttcatt agccgccagt cgtattacct
     2281 cattgttcct tgggctgaag attatctcct ttgtaattgt ttttggttct ttcttcttcc
     2341 aggtcgatta ctccattctg cgcgattcca ccagcaccac tgcggggacg tcttacttcc
     2401 cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc
     2461 ccagcctgat tatttgctat ggcaaacgca aagataagct catcaaaagc gtggtcttcg
     2521 gttcgctgct ggcgctggtg atttatctct tctggctcta ttgcacgatg gggaatatcc
     2581 cgcgagaaag ctttaaggcg ataatctcct caggcggcaa cgttgattcg ctggtgaaat
     2641 cgttcctcgg caccaaacag cacggcatta tcgaattttg cctgctggtg ttctctaact
     2701 tagctgttgc cagctcgttt tttggtgtca cgctgggatt gttcgattat ctggcggatc
     2761 tgtttaagat tgataactcc cacgccgggc gtttcaaaac cgtgctgtta accttcctgc
     2821 cacccgcgtt gttgtatctg atcttcccga acggctttat ttacgggatc ggcggtgccg
     2881 gactgtgcgc caccatctgg gcggtaataa ttcctgctgt actggcaatc aaagcccgca
     2941 agaagtttcc caatcagatg ttcacggtct ggggcggcaa tcttattccg gcgattgtca
     3001 ttctctttgg cataaccgta attttatgct ggttcggtaa cgtctttaac gtgttaccta
     3061 aatttggcta aatctttcag gaagccagcc attcgctggc ttcttgcctg tcaggaaatc
     3121 tcttttgtcc aaatggcaac tcgcctgatt atccttcacc acgtatgctt tgcgtcacct
     3181 tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt
     3241 actttatccc gcagggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
     3301 caatgccagc gaagcgcagt tgcatattgc gttctccgtt tatctggcgg ggatggcggc
     3361 tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaagcctg tcgccatacc
     3421 cggcgcggcg ctatttatta tcgcctcggt gttctgttca ctggctgaaa ccagcgcgtt
     3481 atttcttgca gggcgatttc tacagggact gggcgcgggc tgttgttacg tagtggcgtt
     3541 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cgttactcaa
     3601 cggcattacc tgcatcattc cggtgttagc gccggtgctc ggacacctga ttatgcttaa
     3661 attcccttgg cagagtctgt tctggacgat ggcaacgatg ggcatcgcgg tactgatgtt
     3721 gtctttgttt attttaaaag aaacgcgccc tgcggccccc gcagcttcgg acaaaccacg
     3781 agaaaatagc gagtcactgc ttaaccggtt tttcctcagc cgtgttgtta tcaccaccct
     3841 cagcgtttcg gtgatcctca ccttcgtcaa cacgtcgcca gtattgctga tggaaatcat
     3901 gggttttgag cgcggagaat acgccaccat tatggcgttg accgctggcg tcagcatgac
     3961 cgtttcattc tccacgccgt ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
     4021 ctcacaggtg ttattcctgg cggcggggat cacccttgcc gtttcacctt cccatgcggt
     4081 ttcgctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat
     4141 gagtcaggcg ttggggccgt tttcattacg cgcgggcgta gccagctcga ccttaggtat
     4201 tgcgcaagtt tgcggttcgt cactgtggat ttggctggcg gcggtggttg gtatcggtgc
     4261 atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat
     4321 gttcgtcgcg cctggacgcc cggttgccgc tcatgaagaa atccatcaca acgcttgatc
     4381 tcaatctgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
     4441 agcggatgaa cgtgacccct tcgtcggtga gtaagtcgct ggcaaagtta agagcgtggt
     4501 ttgacgaccc gctctttgtg aactcaccgc tggggctgtc gcccacaccg ctaatggtca
     4561 gcatggagca aaatctggcg gagtggatgc aaatgagcaa tcagttgctg gacaaaccac
     4621 tccacgaaac gccgcgcggt cttaagttcg aactggcggc ggaatcgccg ctgatgatga
     4681 tcatgcttaa tgcgctgtcg aaacggatct accaacgata cccgcaggcg accatcaaat
     4741 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
     4801 tttccggtcg cgaaagtcat ccccgttcgc gggagctgtt aagctcgcta ccgttatcca
     4861 ttgattatga agtgctgttt agtgatgtgc cctgcgtctg gttacgcaaa gatcatccgg
     4921 cactgcatga agcatggaat ctggacacct ttttacgtta tccgcatatc agcatttgct
     4981 gggaacagag cgatacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
     5041 cgattgctat gagcctgccg gaattcgagc agtcactgtt tatggcagca cagcccgaca
     5101 atctgctact ggcgaccgcg ccgcgctact gccagtacta caatcaactc caccaactgc
     5161 cgttggttgc tcttcctctc ccgtttgatg aaagccagca aaaaaagctg gaagtccctt
     5221 ttacccttct gtggcataaa cggaacagcc gtaatccgaa gatcgtctgg ttacgggaaa
     5281 ccattaaaaa tctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt
     5341 cacaatgaaa tgtaaaaaag ttgtaataaa tttatctgaa tcgaactttt agccgcttta
     5401 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
     5461 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
     5521 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc
     5581 gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
     5641 aattgccgga aatcgtcacc ctgccgaaag gaaaaccggt tttcagcgat aaaaatttgc
     5701 cttcgttttc tatttcctat gccgggaata tggtcggcgt ggcgttaaca accgaaggtg
     5761 aatgtggcct cgatatggaa ctacagcgtg cgacacgcgg gtttcatagc ccacacgcgc
     5821 ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta
     5881 acgaagcgcg ggcgcagctc atcacgctgc gccggagcgt gctaaaacta accggtgatg
     5941 ttttgaatga cgatccgcgc gatctgcaac tactgcccat tgccggacgc ctgaaatgtg
     6001 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg
     6061 cggtcacgcc cacgattgaa aagctcagtg tctgggagtt agatggcaaa cacggctgga
     6121 aaagcctgcc cgatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
     6181 cccagctctc taccgtgaag gctttttcgc caaattgata gacaaccaca ggagtcatca
     6241 tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
     6301 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag tatggaagtc aatgcgttgc
     6361 cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagac ggttttccag
     6421 caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc
     6481 cggaatataa ctactcggta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc
     6541 tgccggatca accgctggct ggtaaaccgg tattgattca gaccagctca atgggcgtga
     6601 ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg
     6661 tgatgaacaa gccagaattt atgggcggcg tgattcagaa caaagttgat ccgcaaaccg
     6721 gggaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg
     6781 agtttattca gcgagttaag atctaaataa aaacccgcca gcaatcatgc atggcgggtt
     6841 tttaacgcgc tatcgatttt agtgagcgtc gataaagaca atcttcagga taaacagcag
     6901 cgcaacgatg attacgcacg ggctaaggtc acgcagacgt ccggtaccaa tcttcatcac
     6961 gcagtaggag ataaagccca gcgcgatacc ttcagtaatc gagaagctga acggcatcat
     7021 cacagcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     7081 acttgaggtc atcaacacgc caacgtaaat cagcgcacca gctgcagcgt agcctggcac
     7141 catccccgcc agcggcgaca ggaagataac cagcaggaac agcagaccaa caaccactgc
     7201 cgtcagaccg gtacgaccgc caacggaaac gccggaagaa gattcaatat acgccgtaac
     7261 agaagaagta ccgataaacg aaccggtcac cgaagagata ctgtcgacat acagcgcctg
     7321 cttcatgcgc gggaatttac ctttctcatc agccagacct gctttatcgg tcacgccaat
     7381 cagcgtaccg gaggagtcaa acaagttgac cagcatgaaa gagaaaatca cccctgccag
     7441 cccaaggtta aacgacccgg ctaaatctac atgacccacg acactcatta cgctcggcgg
     7501 cgcagaaacg atgccattat agtggacatc acccagcatc cagcccagca gcgttgtcac
     7561 cacgatggaa accagcaccg ccgcgtgaat attgcgcgag gccagaatgg cgatgatgaa
     7621 gaagccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     7681 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa taaacagacc
     7741 gataccactg gtgatgccca cacgtagact taccgggatg ttggctatca tccagtagcg
     7801 aacgcggaaa atcgtcagta acagcagacc aatcgcgccc cagaagattg cgcccatccc
     7861 gacttgccac ggcaagccca tcgcctgtac aacgacaaaa gcgaagaacg cattcaggcc
     7921 cattgccggt gccagtgcaa ctggcaggtt agcaaacagc cccatcataa tactgccgaa
     7981 tgcagcaatc agacaggtcg tgacgaagac ggcgctggta tccatgccag caacgccaag
     8041 aatttgcggg ttaacaaaaa cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat
     8101 cacttcggtc cgtgccgtcg tgccatgttc gcgcagttta aacacgcgtt ccagcatccc
     8161 ctggccagaa gcctgggttg tgtgttgttg actcattatc tatttccgaa caaggaggga
     8221 aaatccgtcg ctatcgtata ccaaaatgag acaatatgga cggctgaaga taactttttt
     8281 tcattgtatt tgcttatacg gcaacgattg cgtcatgcga attggcatga cgaaaacgtt
     8341 aaactaacga taaaggaaag acatgtccca gatagaagcg gtatttttcg actgcgacgg
     8401 tacgctggtc gatagcgagg tcatttgctc tcgcgcctat gtcacgatgt tcagggaatt
     8461 tggcattcac gttgacctgg aagagatttt tacccgtttt aaaggcgtaa aactctacga
     8521 aatcatcaac atcatcagta aagaacaggg cgtgacgatg atccaggccg atgccgagca
     8581 cgtttaccgt gcggaagtgg cgcgcctgtt tgatagcgag cttgaggtga ttgcaggagc
     8641 gaacgaattg ctggctagtg tgaaagtgcc gatgtgcgtc gtctctaacg gcccggtcag
     8701 caagatgcag cattcgcttg gtaagctcaa catgctgcac tacttcccgg aaaaactgtt
     8761 cagcggctat gatatccagc gttggaagcc cgatccggca ttaatgttcc atgcggcaaa
     8821 agcgatgaat gtgaacgttg aaaactgcat tctggtcgat gactccagtg ccggtgcgca
     8881 atcagggata gatgccggaa tggaagtgtt ttacttctgc gctgacccgc acaataaacc
     8941 gatcgttcac ccgaaagtca ccacctttac ccatctttcg caattaccgg aactgtggaa
     9001 ggcgcgtggt tgggatatta cggcatagtt attcacactc ccttcactta ctccacttaa
     9061 attggcacgc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc gggtaattca
     9121 tcacggactg tattttgcgg ttttagggcc gttaattggt gttctgtttc ttgtcctcta
     9181 catattcttc gcaaaagaac cgctggttct ttgggtgata atacatccaa tttttctctt
     9241 attgtcgata actacgggag ctattcctgc gttgttaacc ggtgtaatgg ttgcctgtct
     9301 gccggaaaag atcgggtcac agaaacgtta tcgttgtctg gctggtggca taggtggagt
     9361 tgttatcacc gagatctatt gcgctgttat catacatatt aaggtcatgg cttcctcagt
     9421 gttgtttgaa aacattcttt ctggtgacag tctcgttgtc cgcatcattc ctgcattgct
     9481 ggcaggtgtg gtgatgagca gaatcattac acgtctaccc ggactggata tttcatgtcc
     9541 tgaaacagac tctttaagtt aagcgggatg ttttatcttt tggctactca aaagcagaca
     9601 ggatgtttct atgactcaaa atatcaggcc gttaccccta ttcaaatatc atcccaagcc
     9661 actggaaaca ggcgcatttg aacaggataa aaccgtagag tgcgattgct gtgaacaaca
     9721 gacgtcagtt tattactcgg gtccctttta ttgcgttgat gaagttgaac atctctgtcc
     9781 gtggtgtatt gcgaacggtt ctgctgctga aaagtttgca ggtagttttc aggatgatgc
     9841 cagcatcgaa ggtgttgaat ttgagtatga tgaagaggac gattttgccg gtattaagaa
     9901 cacatatcct gatgaaatgc tgaaagagtt ggttgaacgc acgccaggtt atcatggatg
     9961 gcaacaggaa ttctggctcg cgcattgtgg cgatttctgt gcttttatcg gctatgtggg
    10021 ctggaatgat ataaaagatc gcctcgatga atttgccaac cttgaagaag attgtgagaa
    10081 tttcggtatt agaaattctg atctagctaa atgcctgcaa aagggtggtg attgtcaggg
    10141 ttatctcttc cgctgtctcc actgcggcaa gctgagactg tggggcgatt tttcatagtt
    10201 atttatatga tgagaacagg ccggcgcgta attcacacat ccggccttat ttttaagctt
    10261 aaccgagcgc taattctgcg gcagcggctt tatcagcgat taccatcagc gaaggatgca
    10321 gctgcaacac ggaagcagga acatcttcgg taaccggacc ttgcagaaca tttttcagtg
    10381 cctgcgcttt acccgcgcca ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt
    10441 tcggccccat cgtcacgtag ctgtcaggca ctaatgaaaa gtcgccaccc aactcgccat
    10501 gcgccacaat atcaaccatc tctccctgga tcgggaactc cacggtttgc tcatggaaat
    10561 gggtcgtatt tggcaaattg ccacaaaaat gaccatctac acctaatccc aacaccacca
    10621 aatccaggcc accttcacgc gccagtttct gatcatgctc gcggtagtta tcaatagtga
    10681 gcttctggat attctcttct ttgatccctg caggggtgaa aaacagatta cgcagattgg
    10741 taatcgttac gccttcccct tctttgccac gaaatggaat ttcatcgaaa ttgtagaaat
    10801 agcagttatc gtaccagggc ttacctttaa ccagagtgat gagatattcg tacatgcctt
    10861 tgggcgtgct accggcggta attgccaggt taacgcgacg catctttgac atataaccaa
    10921 gcagatgatg tgcggcgaca cggctcattt cctggtaatc ttcggtaatg atcaatttca
    10981 tcttaatgcc ttattttatt taaataattt ctgtacaaag gctgcatagg ctggtcgcca
    11041 gacatccatt tcatggttca gaccgggata ttcctggtaa tcaaacttta tttttttctg
    11101 ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt tatcgcccac
    11161 gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca gccgggcagc
    11221 gaccccttca tctggtacgg tcgtcgtggt aacaccactg aatgtggcca gccagccaaa
    11281 gctttccaga tgattaattc ccgataccag tgcctgatac ccaccttgtg aaagccctgc
    11341 cagtgcgcga ccatcggcat ctttacggac attaaaacgc ttgctaatca gcgggataat
    11401 atcgttcatc agttcgcgat ctgccgcttt agcatttaac ggataaaaga ctttacgtct
    11461 ctcctgagga acgaaatctt cgggaataat gcccttcgca tcggtttcgg tatccgggat
    11521 caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga tttgcgggat
    11581 acgcccctga tcaatagcgg aacgtccggt atcaccaaag ccgtgataga aatagagcac
    11641 tggcaaaggc tcgcctatgc cggtgtatcc tggcggggtc cagacataca tctgacgttc
    11701 ggattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgca caacagaacg
    11761 tgtatccaga taactgcccg gtaccagaat catactggag ttaacctgac gctgaggctt
    11821 agtcattgcc gtgcctgtat caatgctgcg tacaccatca acattgaaaa aatactcgta
    11881 cagattgcct ttcaggacag gcgtgcgcca cgaccagact cccgcttcat ctttggtcat
    11941 cgggtgaata ttgtccggaa ccggaacacc caccactacg gagacatttt ttgccccagg
    12001 ggcaaagtag cgaaaggtca cgctgttatc ggtattgacc tgagtcacat actgtttcac
    12061 cggaatagtg ggtgccgggc tggctggcat atccgctgct atggcccatt gagcagaaat
    12121 accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa tattcctttt
    12181 attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg gtcgtcttta
    12241 ttgtcttcaa aggtgaagcc atccagttcg ttttccagag ctttgatata tgtagcgtag
    12301 aaacgaattt ccgggcgcga ggtcaacata ctggtattga ctttaaaggt atggaagagc
    12361 gtggttttat aaccagactc attatattta ttgctattcg catctttgtt ttgctgggtg
    12421 aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc cgggcgaaca
    12481 accgcacgaa tagattcgaa atcagaatgt gcgcctgttt cgtagctata aatatcgtta
    12541 ccgaaggagt aaacaatcgc gttagccaca atgaaatgat cgccgatata agcttcgccc
    12601 tgcgaagtca gacgaactgc agttccgcct gtgtgatcac cataataacg accattaaag
    12661 gtagtaaatg gacttgcacc agcataacga ccaaagttac tggcgataga gttattcgca
    12721 accaggaagg agaattcgtt gaagccaccc ttatcaaatt tctgcgttaa agatgtacca
    12781 aacatccagg tatctttcca gtcataatac ccgttattat cctgattatc tttttccgat
    12841 gcgctttcat ttgctgagac ataacgaccg ctcaccatta aggtggcttt atcccataac
    12901 gggatatctt tatagcgtaa atcaatggta ttggtattaa tctgctgttt gttttgcagg
    12961 ctgcgatcgt aatcatcgac atcttcacga accagcgcga tatcaatttt acccggaccg
    13021 actttccagt tttccagacc tacacctgcg gcagcatcgg tacgctgcgt tttccagtca
    13081 agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc agcctctggc
    13141 gcaaagggca ggaaaccttt ggtggtaacg tacatatcag agaactgcat aaagttctcg
    13201 ccaccggcgt tatcgccaaa ccagccagta gagtactgct gacccacgtt accatccatc
    13261 atcacgatgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt taactgcaaa
    13321 tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaattgc ccatgattta
    13381 ggtgaaccat gggaggctgt gccccaacca gaacggtagt agccgttgta actaaagccg
    13441 atttcatctt tcacaaattt actgaaatct ttcagagtca tggaagaata aatcgagcct
    13501 gtctcactgg catttttctc ttcattttta accagtacag catcaggctt tgcggcacga
    13561 ctggtcggga atggattggc ggcataccct tgatcattcg cacttacgct acgattcacc
    13621 gtcgctggcg tatacttttt cttctcttca tctttatatt ttttcagttc gctttgcgtt
    13681 tctcttaacg ccttttctaa tagctcaagg cgttgttcca cggttaatga ttcagccaat
    13741 gattgtgcgg aaaaggctaa cggtgccatt aataaaatgg cagaggtaat aatatttcgt
    13801 ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgcatcgcat ccagactgtt
    13861 ctgaatgcga cgatatttaa ggtgctttat tggttatttt tttaatgata gcccccgagt
    13921 tttgatcact tctgcatacc atccgaagct tttcttgcgc gttcttgtga ggcttccttc
    13981 accattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt gtgaatgaga
    14041 tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca caccatcggc
    14101 aatcgcttca tttacctgta ccaggtgatc atttaaatag gcaattcgat aatcgtcctg
    14161 tatcgaacca tccacttcaa cgctgtcttt tgcgcctaat ccgttctcga caataaataa
    14221 cggtttttga taacgatccc aaagcgtatt taacagaatc cgtaatccaa ccggatcaat
    14281 ttgccacccc cactctgaac ttttcagatg cggatttggg atcatattca gtatgttgcc
    14341 ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccggtcatgt aataactaaa
    14401 agagatgaaa tctacggtat gttttaaatc ttctgcgtca ctttcagtca tctcaatggt
    14461 gatattgtgg tcgcggaaga aacgctgcat atagccggga tactgaccac gcgcctgaac
    14521 atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat cctgtggctg
    14581 gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccaattttcg cttcggggat
    14641 caggctatga caggctttaa ctgcccgcgc actggcaacc agttgatgat ggatcgcctg
    14701 ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg gcgcgtgtaa
    14761 tgacatgttg atttcattaa atgtcagcca taacacaact ttatgttggt agcgggtaaa
    14821 gaccgtgcgt gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc aaccgccgta
    14881 gtttttcacc agcccatatg gcatttcgta atgggataac gttaccagcg gcttgatccc
    14941 cgcctgcgcc atttcatcaa acagccgctc gtaaaacgct aaccccgctt cattcggttc
    15001 ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa
    15061 gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat cgatggcgac
    15121 atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta cgccatgagg
    15181 ctgtaaatct gaggtcgtga tccctttgcc atcttcctgc catgcgcctt ccacctgatt
    15241 ggcagctgtt gcaccacccc aaagaaatgt ttctggaaat gctttcataa ttaactcctt
    15301 ttatcgttag cgaatgatgg ataacagcgg ctcaccagcg tttatctgca ccgtgccgtg
    15361 gggtaatacg tccgtaaaat catcgctatt actgattaat accggcgtcg tcagatcaaa
    15421 tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg tattgacctt
    15481 gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc
    15541 gacatgaatc aggatctcca cgccatcatc tgactcaatg ccaatggcgt gtaatgtggc
    15601 gaacaacgaa gcaattcgac ccgccaccgg agaacgcact tcaccaacag agggcagaat
    15661 ggcaatacct ttacccaaca ggccactggc aaacgtggta tcagcgacgt gaatgagcgg
    15721 cacaatatct cccgtcatcg gtgaacagat accgccctgc tcaggtgtaa taatctctgg
    15781 tgttttctct tgctgggcaa cttgcgctgg ctgacgttta gcggtgatga aatgaagcat
    15841 caccgtaccg acaaatgcgc aaccgatggc aatgacacca ccaataacgc tggcccatac
    15901 ggtgaaatca atccccgttg acgggatggt ttgcatgaag gtgaaaatac ctggcaaacc
    15961 aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg gcccccaatg ccccacttat
    16021 gcaggcgata acgaaggggt acttacgcgg caggttgacg ccatatactg ccggttcggt
    16081 gataccaaac agccccgtca acgccgccga tcccgctacc acttttttct gcgcatcgcg
    16141 ttcgcagagg aagacgccga gcgccgcccc gacttgcgcc ataatggcgg gcattaacag
    16201 cgggatcatg gtgtcgtagc ccagcacggt gaagttattg ataattaaag gtatcaaacc
    16261 ccaatgcaga ccaaacatca caaagatttg ccagaagccg cccattaccg cgcctgcaaa
    16321 tgcaggaacc gcctgataaa gccagagata accggcagca atcagttcac ttatccaggt
    16381 tgatagcggc cccaccagca gaaatgtgat gggcgtgatg accatcagac atagcaaggg
    16441 tgtgaagaaa tttttgattg ccgaaggtaa ccacgcatta agtcggcgtt ccagaatgct
    16501 gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca acaatgtgac
    16561 cggaataccc aggaaatcca ggcccagcgc atccactttt tgcccgttct cgaaggcagt
    16621 cagaattaat ggatgcacta acgctccgcc aatcaccatg gcagtaaatg gattgccgct
    16681 gaagcgtttc cctgcggtgt agcccaggat tatcgggaag aaccaaaaca aggcatcact
    16741 ggcgctaaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa aagtgagtgc
    16801 cagagccaac atacctttca ggatcccggt tgccgccatc aaaccgatca gaggcgtaaa
    16861 aatacctgaa ataacataaa caaagcggtt taacagatta cctttatcat cattttccgg
    16921 tgcctgttgc gctttttcgc caaggcctgc cacgctgtta accgccagga agacatcggc
    16981 cacatggtta cctatgacaa cctgaaactg gccaccgctt tccaccacca taataatacc
    17041 gggggtcttt ttcagtacct cagcttgcgc tttgctttca tcctttaact taaaacgtaa
    17101 tcgcgttgca caatgcatca gactcacaat gttatctgcg cccccgactc ctgcgactat
    17161 tgttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa ctcaaaaaaa
    17221 aacctgaaaa aaacggcctg acgtgaatca agcaattttt ttcaggtttt gcccgcttag
    17281 tgcggtaaca atcctttatt cagtaataat atttcagtgt tctttgcgca cgcgctctat
    17341 atttatggct aaaaacataa tctctgcagg tgagatttta cgttgatact gcaaaccaat
    17401 aaaaatggcg atccgctccg cacattgcca tgcttgcggg taattttgct ttactgctta
    17461 ttgtaatgat tcatcactat cgttaatcga tgcatgttca ataatacgcc aggataaaaa
    17521 tttcagatgc gtaaccagtc gctgataact caagctttct tcctggtaat taaggctgaa
    17581 ctgaaatttt attaattgca gcatttctcg cattaattgc gtgacacctg caacatcctc
    17641 catatttccg ctcatttggg cactgaccag atgcatggca ataaagccca cttcatcttt
    17701 cggtaactgc acgcccaacc gtttatcaat aatggttaac gcttcttccc ctagctggaa
    17761 ctctttcggg taaagtcgct ggatatccca cagcagcggg ttgggcagta gcacgttttg
    17821 ctgaaagcgt ttaatcgcaa actggcaatg gtcagttagc gagatataaa tactgtcctg
    17881 caactttccc agacgctcct gcgccagaga gataatacga tcacaggttg ccatcacctc
    17941 aagaggcata tgacttaaga gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc
    18001 atactctttt tctattccac ttgagttaat tctttcgcct gggcgttttt gaaagccaat
    18061 tccgcgcccc atgacgactt tttcccgctg ttgatcatca ataaccacca caacattatt
    18121 gttgagaatt ttggtgattt gcatgttcat agcaaggacc ttttttataa acaaaaaaac
    18181 ccgacttcac cagtattctc tggttatgtc aggttttgcc tgcgaatgca gtaacaatcc
    18241 agtcattaat taatggtttt tataacgaac atccaggttc gaaaattaat ttagttgcgt
    18301 gcagaaaaaa tagcaatgct ctatttataa aagtatgacc atgctcgcag ttattaattt
    18361 tgtgtaat
//
LOCUS       DAPQZD010000091        18155 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPQZD010000091 DAPQZD010000000
VERSION     DAPQZD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.091"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(321..911)
                     /locus_tag="RO454_003024"
     CDS             complement(321..911)
                     /locus_tag="RO454_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934824.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(961..2157)
                     /gene="hemY"
                     /locus_tag="RO454_003025"
     CDS             complement(961..2157)
                     /gene="hemY"
                     /locus_tag="RO454_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDY0934825.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2160..3359)
                     /gene="hemX"
                     /locus_tag="RO454_003026"
     CDS             complement(2160..3359)
                     /gene="hemX"
                     /locus_tag="RO454_003026"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDY0934826.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(3381..4121)
                     /gene="hemD"
                     /locus_tag="RO454_003027"
     CDS             complement(3381..4121)
                     /gene="hemD"
                     /locus_tag="RO454_003027"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDY0934827.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4118..5059)
                     /gene="hemC"
                     /locus_tag="RO454_003028"
     CDS             complement(4118..5059)
                     /gene="hemC"
                     /locus_tag="RO454_003028"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDY0934828.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5446..7992
                     /gene="cyaA"
                     /locus_tag="RO454_003029"
     CDS             5446..7992
                     /gene="cyaA"
                     /locus_tag="RO454_003029"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDY0934829.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(8032..8352)
                     /gene="cyaY"
                     /locus_tag="RO454_003030"
     CDS             complement(8032..8352)
                     /gene="cyaY"
                     /locus_tag="RO454_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDY0934830.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8478..8663)
                     /locus_tag="RO454_003031"
                     /pseudo
     CDS             complement(8478..8663)
                     /locus_tag="RO454_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8822..9025
                     /locus_tag="RO454_003032"
     CDS             8822..9025
                     /locus_tag="RO454_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0934831.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9062..9886
                     /gene="dapF"
                     /locus_tag="RO454_003033"
     CDS             9062..9886
                     /gene="dapF"
                     /locus_tag="RO454_003033"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDY0934832.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGIGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9883..10590
                     /locus_tag="RO454_003034"
     CDS             9883..10590
                     /locus_tag="RO454_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDY0934833.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10587..11483
                     /gene="xerC"
                     /locus_tag="RO454_003035"
     CDS             10587..11483
                     /gene="xerC"
                     /locus_tag="RO454_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDY0934834.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11483..12199
                     /gene="yigB"
                     /locus_tag="RO454_003036"
     CDS             11483..12199
                     /gene="yigB"
                     /locus_tag="RO454_003036"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDY0934835.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12283..14445
                     /gene="uvrD"
                     /locus_tag="RO454_003037"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12283..14445
                     /gene="uvrD"
                     /locus_tag="RO454_003037"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDY0934836.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14500..15402)
                     /locus_tag="RO454_003038"
     CDS             complement(14500..15402)
                     /locus_tag="RO454_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDY0934837.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15485..16249)
                     /locus_tag="RO454_003039"
     CDS             complement(15485..16249)
                     /locus_tag="RO454_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDY0934838.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16619..17569
                     /gene="corA"
                     /locus_tag="RO454_003040"
     CDS             16619..17569
                     /gene="corA"
                     /locus_tag="RO454_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDY0934839.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17612..17992)
                     /locus_tag="RO454_003041"
     CDS             complement(17612..17992)
                     /locus_tag="RO454_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDY0934840.1"
                     /translation="MSKDYMADGSLTEKWKYRFSFYDEHGFPGFWGASPEYKQALKGL
                     NTRQKIIIRTNVIAFFFSWVYLFVLGLWKKAIVVLFLAIFSLIFGYMIGYNVFGFIIA
                     AYVSYNTNRWFYEKEVKGIHTWSL"
BASE COUNT         4241 a         4729 c         4782 g         4403 t
ORIGIN      
        1 aggagaaata ttcactcagg aagttattac tcaggaagca aagaggatta cagaattatc
       61 tcataacaag tgttaaggga tgttatttcc cggttctctg tggcataata aacgagtaga
      121 tgctcattcc atctcttatg ttcgccttag tgcctcataa actccggaat gacgcagagc
      181 cgtttacggt gcttatcgtc cactgacaga tgtcgcttct gcctcatcag acaccatgga
      241 cacaacgttg agtgaagcac ccacttgttg tcatacagac ctgttttaac gcctgctccg
      301 taataagagc aggcgttttt ttatgaccga gtaaacaaca ggctacgtcg ctcttgggtc
      361 atcggttgtg cgtttaatac acttacaaga gaacctaccg tggtgatggg aagaaacgga
      421 ttatcttcta ttccgcgtag atcgcgccca caacgagcgg caatatcttc gaccagctct
      481 tctaactcgt ctgcatcgtt tactaaatcc agatcatcgt ccagtttatc actggctttc
      541 agtggtaatg gataatcgat atagccgttt ccttgtaagg tattccacac agcccgaatc
      601 acccatgtat ctacggtgtg gggggaaaat tggcgtgcga actgacaaat ggaaagtccc
      661 tgacgtttat cagccataat ctggcgcttc tcgatgagct tgattttttc ctgcttcgca
      721 atggctatca gatacccaat aaaaagtgtt aaccagaaga tgaatgtatt gcctgtacgt
      781 acgaaggaca gaatcagcca acttacgcca gccagaacgg cacatatcaa caaaatcagc
      841 aagagcgttg cccgaaaagg attaatttca tgtgatggta agcggaaaat atagcgggac
      901 ggtgtgcgca tgtttgacat ccttgtaaaa aaatatcgcc cctgaacgag gcgatataga
      961 ttactgcggt gggttgtttt gcaacgttag catcaaaccg tcgcgacgca tcgctgcggc
     1021 ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat cgtaggcgtc
     1081 cggacgttgt ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt ctccgtgttt
     1141 catcagcgac tgacccagtg tgctccacaa cagcgggcga tcgcccacgt ttttgatctg
     1201 ttggcgcagc actttttcca gctgttccgg attattggtt ttcagacgcg ggatcggcag
     1261 cagcaggcga tcgtcatact ggcgtttcag gccgtcgatg ataatttgct gggcagtatc
     1321 atgatcgtca cactcaataa gatgttccgc cattgccacc tgcaaggcca cctgatgacg
     1381 cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcgctaccgt tatcggcacg
     1441 cgcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat gttcttcatc
     1501 accaacatgg gctttcgcca ttgatgggat gatatccagc agcgaactcc atgcccctgt
     1561 gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg taacttccag
     1621 cagcttatca acgccgtgac gtgctgcatg gttttcatta cgggccagtt gcagacgtac
     1681 acgggtgatt tctaccggaa tggtgtcatt gccggccagc tccgctgcgc gttccagatg
     1741 ttggttggcg cgtgcttcat caccacgttg ttgcgcggct tcggctgcca gtagatagtt
     1801 caccaccggt tgttcagcgt gatcggcatt tttcgccatc agcttttcaa cttgctgata
     1861 atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgtgcac ggcgacgctt
     1921 acgtccgaca aaccacccac gggtgtgcgc gcctgtgcgg aagattcgcc gcagtagcca
     1981 ctcaatggca aacagcacta ccatcgccag aatcaaaata atcgcgaggc ccgtgacgct
     2041 agtttcaata ttgtagttgt cggtctggat cagcacgtaa ccctgatggc cggcaatcat
     2101 cgggccaacc acgatccccg caatcagcaa cacaaagagc aataacactt ttagcatggt
     2161 tattctcctt gcggcgcggc tgccggagca tcagcttgcg gtgcaggtgc aggtgcaggt
     2221 gcaggtgcag gtttagcttc cgttgtccct gctgccggtt gtgccagcag gttacgcacg
     2281 cgggtctgca tcaatttttc cagcatcgcc tggctttgca gggtttccgg aagatccatc
     2341 gagatatttt gctggcttaa ctggtccacc tcgtcgagga acgctttggt ggtggcatca
     2401 tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg gcgataagtc
     2461 tcttcctggt gacgcggtac agcttgtgct gcgaccagca ggcgagagcg gatattttcg
     2521 cgcagataga tatcctgatt tggcgctaac agcggtacgg cagtgtcatc acgacggcga
     2581 atcgtaatga agttgtccat aaaattctgc cagctttttt gcagattgat acgccattcg
     2641 ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc gctgtcatta
     2701 tcggccagac gcagattatc tacctgattt gaaagctgat taagcttaag gatgataccg
     2761 tcataatcca cctgcgatac cgcagaaagg ctggcgatat catcggtaat tgcccggcga
     2821 acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact tttcagcaac
     2881 gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag tttcaccaga
     2941 aaatcggcct gggccagcag ccaggtttta gcatcgctgc cggaaatggt ggcgaccttt
     3001 tgttggactt catccagctg ttttgccagc gtttcttgct gacgattcgc ctgctcaagc
     3061 tgtacagctt gttgcttgat aatgccttcc agctcggctt tttggctctc ctgggctttt
     3121 tgcagtgccg taagttggtt agccagggca tcgctggtgg cagtctgatt gacggcctgt
     3181 tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat agccaccgcg
     3241 ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg ttgtgacgtg
     3301 gtgtccacgg cctccctggt ctcttcaacc acggcggagg ttttttcttg ttccgtcatt
     3361 atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt gtcagcgtta
     3421 tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa acgctcactg
     3481 acgaccaata gtcgacagcg tagtaaccag tgctcacgat accattgtgg gatcagcgac
     3541 cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc gcgggattgc
     3601 cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata acattcacaa
     3661 aaagtgacct cagcaccgcg cgccgtcagg gaatccccaa ttagctcacg accgccattg
     3721 ccacgtaata tcagcgcaca tttgcccgca atattttgta attcaggtaa ttgtagcaag
     3781 acttcgctga tttcccgatc ctgtgggtag agaatcttct gtccgcttac ggtatgtagt
     3841 gccagcgcgg tggtgcgtcc aatggcgaaa taagtaggta gtcggggcca tttacgatct
     3901 tgctgatgca gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc aaacaacaga
     3961 tcgctctctc ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg acccggagaa
     4021 aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg gctcactaac
     4081 tcttctcctg cgggagacgg gcgggtgacc aggatactca tgccggggcg tctccgttat
     4141 agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc agcgaaatcc
     4201 ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc agcgaaccgt
     4261 ccggcgcgcc gaccaacgca cgcagccaga tttcgccatc aataagctcg gcgtagctac
     4321 caattggcac ctgacatccg ccttcgagac gggtattcat ggcgcgttcg gcggtaacac
     4381 gcagtgcagt ttcgtggtga ttcagcgcgg cgagcagctc gcgagtgcgt gcatcatcaa
     4441 ggcggcattc aatgcccacc gcaccttgcc ctaccgccgg aagagaaatc tcgggtggca
     4501 acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg gcaagaatga
     4561 tggcatcgta ttcaccgtta tccagtttgc tcaggcgagt gccgacgttg ccgcgcaggg
     4621 agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt aaactggacg
     4681 tcccgacgat actgcctgcc ggtaacgcat ccagactgtc atagttattg gacacaaatg
     4741 catcgcgagg atcttcacgc tcgcaaatag tgaccagccc cagaccttgc gggaattcaa
     4801 ccggcacatc tttcatcgaa tgtacggcga tatcggcgcg attttcgagg agcgcgactt
     4861 ccagctcttt aacaaataag ccttttccgc ctactttcgc cagcggcgta tcaagaatca
     4921 catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg ctcgccatca
     4981 acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt gtggcaattc
     5041 ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca tcctaacatc
     5101 cttgccagag cgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa ccgtaaaatg
     5161 aggtcgggca gtggatcctg acaggcgctt tgagccttcg taataaggaa tttacagaga
     5221 ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt
     5281 aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca ggcgcgaaaa
     5341 gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta ccgttgatgt
     5401 tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta cctctatatt
     5461 gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg cgcgcttgct
     5521 gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt gcactatcac
     5581 catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg cctttacacg
     5641 cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg aatgtcagta
     5701 caggacccac cgaaaggtga gcttccaatt actggtgtat acaccatggg cagcacctcg
     5761 tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca atcctggctc
     5821 gatagcgaag agcgccagtt gctacaacgt aaatgtagtc tgctggaaag ctgggccgcc
     5881 tcgctgggtg tggaagtcag cttcttcctg attgatgaaa accgcttccg tcataatgaa
     5941 agcggcagcc tggggggcga agattgtggc tccacccagc atatactgct gcttgacgaa
     6001 ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat ggtgccgtgc
     6061 gacgaagaag agcattacga cgactatgtg atgacgcttt acgcgcaggg cgtgctgacg
     6121 ccaaatgaat ggctggatct tggtggctta agctcgcttt ctgctgaaga gtactttggt
     6181 gccagccttt ggcagctcta caagagtatc gattccccat acaaagcggt gctgaaaaca
     6241 ctgctgctgg aagcctattc ctgggaatac ccgaacccac gtctgctggc gaaagatatc
     6301 aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgacccgta ctgcatgatg
     6361 ctggagcgtg ttactgaata cctgacggcg attgaagatt tcacccgtct ggatttagtg
     6421 cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc gtgaacgcgc ctgtgtaggc
     6481 tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg gttgggacga agctcgtctg
     6541 gcaatgctcg ataaccgcgc taactggaag atagatcagg tgcgtgaggc gcacaacgag
     6601 ctgctcgacg caatgatgca gagctaccgt aatctgatcc gctttgcgcg ccgcaataac
     6661 cttagcgtct ccgccagccc gcaggatatc ggcgtgctga cacgtaaact gtacgccgcg
     6721 tttgaagcat taccgggtaa agtgacgctg gtaaacccgc agatttcgcc cgatctctcg
     6781 gaaccgaatc tgacctttat ttatgtaccg ccgggccgcg ccaaccgttc aggttggtat
     6841 ctgtataacc gcgcgccaaa tattgagtca atcatcagcc atcagccgct ggaatataat
     6901 cgctacctga ataaactggt ggcgtgggca tggtttaacg gcctgctgac ctctcgcacc
     6961 cgtttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga gatggtcgca
     7021 gacgtgtcgc accatttccc gctgcgctta cctgcaccga caccgaaggc gctctacagc
     7081 ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc gacggcagcc
     7141 ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag ctttggcgag
     7201 aatcaaaatt gcctggtggg tagcgttgac ctgctgtatc gcaactcgtg gaacgaagtg
     7261 cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac tattctcggc
     7321 aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta tagccagcat
     7381 ctgcgcgggt taatccgtac tcgcgtgcag caactggttt ctgagtgtat tgaactgcgt
     7441 ctttccagca cccgccagga aaccgggcgc ttcaaggcgc tgcgcgtctc cggtcaaacc
     7501 tggggcttgt tcttcgaacg gctgaatgta tcggtacaga aactggaaaa cgccatcgag
     7561 ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt tgaaaccaat
     7621 cacgtcaaat taccggcggt ggtggacggc tttgccagcg aagggatcat ccagttcttc
     7681 ttcgaagaaa cgcaagacga gaatggcttt aatatctaca ttctcgacga aagcaaccgg
     7741 gttgaggtgt atcaccactg cgaaggcagc aaagaggaac tggtgcgtga cgtcagtcgc
     7801 ttctactcgt cttcgcacga ccgtttcacc tacggctcaa gcttcatcaa cttcaacctg
     7861 ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc gtttcgcacc
     7921 aaatctatcg gtaacatgcc acctgccaat caggagcatg acgcgccgct gttgcagcag
     7981 tatttttctt gatgctaatg ccggaaagcg aggcttatcc ggcattagct cttaacgaaa
     8041 actgacggtt tctcccgcct gctgcgtcgc cgcctgttcc agcaaatccc agaaggtttc
     8101 gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc cgccctgttt
     8161 ggttgccagc catacctggt gcagtggttc ctggcggttg ataatgattt tgctgccgtt
     8221 ctcaaaggta atggttagta caccgccgtt gatttcacag tcgatatcgc tgtcgccatc
     8281 ccagtcgtcc agacgttctt caatggtcag ccacagttga tcagccaggc gatgaaattc
     8341 actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc ggcatggggt
     8401 cagggcttca aagtttgccc gtctgctgct gcgttccggc atgattcatc cgtcaccgga
     8461 ataataatat ctctatatag cgaaagattg gtcttttcat aagggcgcaa atacaccacg
     8521 ccttttccct gtttctggta aatattatgt tccggctcat caagccgaca tccccatgcc
     8581 gatgataaga acaacgaaca agacaataaa caaaccacat tgcgatagtg cataaagcca
     8641 tcctggcgcg aggtgccgat cacgaaacta ccagcaaaac ataaatcgcc acgagtaagc
     8701 gttatactcg cagcatttcc tcacttttca gacttcataa agagtcgcta aacgcttgct
     8761 tttacgcctt ctcctgcgat gatagaaagc agaaagcgat gaactttaca ggcaatccat
     8821 aatgaaaaac gtgtttaagg cactcactgt attacttact ctcttcagcc tgacgggctg
     8881 cggtctgaaa ggtccgctct atttcccgcc tgcagataaa aacgcaccgc cgccgaccaa
     8941 accggtagag acgcaaacgc aatccacggt gccggacaaa aacgaccgcg ccactggcga
     9001 tggtccatcc caggtgaatt actaaaagtc agtttctgta cccgcgtgat tggagtaaat
     9061 gatgcagttc tcgaaaatgc atggccttgg caacgatttt atggtcgtcg acgcggtaac
     9121 gcagaatgtc tttttttcac cggagctgat tcgtcgcctg gcggatcggc acctggggat
     9181 agggtttgac cagctgctgg tggttgagcc accgtatgat ccggaactgg attttcacta
     9241 tcgcattttc aatgctgatg gcagtgaagt agcgcagtgc ggcaacggtg cgcgctgctt
     9301 tgcccgtttt gtgcgcctga aagggctgac caataagcgc gatatccgcg tcagcaccgc
     9361 caacggacgg atggtgttga ccgtcaccga tgatgatctg gtccgcgtca atatgggcga
     9421 acccaacttc gaaccttctg ccgtgccatt tcgcgctaac aaagcggaaa agacctatat
     9481 tatgcgcgcc gccgagcaga caatcttatg cggcgtggtg tcgatgggaa atccgcactg
     9541 cgtgattcag gttgatgatg ttgatactgc ggcggtagaa acgcttggtc ctgttctgga
     9601 aagccacgaa cgttttccgg agcgcgccaa tatcggtttt atgcaagtgg ttaagcgcga
     9661 gcatattcgt ttacgcgttt atgagcgtgg ggcaggagaa acccaggcct gcggcagcgg
     9721 cgcgtgtgcg gcggtggcgg tagggattca gcaaggtttg ctggccgaag aagtacgcgt
     9781 ggaactccct ggcggtcgtc ttgatatcgc ctggaaaggt ccgggtcacc cgttatatat
     9841 gactggcccg gcggtacatg tctacgacgg atttattcat ctatgaagca accaggggaa
     9901 gaactgcagg aaacactcac ggagcttgat gaccgggcgg ttgtcgatta tctgattaaa
     9961 aatcctgagt tttttatccg caatgcgcgc gcagtagaag cgatacgtgt gccgcatccg
    10021 gtacgcggca ccgtttcgtt ggtcgagtgg cacatggccc gcgcacgtaa tcatattcat
    10081 gttctggaag agaacatggc gttgttgatg gaacaggcta tcgccaacga aggcctgttt
    10141 tatcgtctac tctacctgca gcgcagtctc accgccgcca gcagtctcga tgatatgctg
    10201 atgcgctttc accgctgggc gcgcgatctc ggcctggcag gtgcgagtct gcgcctgttt
    10261 ccagatcgct ggcgcttagg tgcgccgtcg aaccacactc atctggcatt aagccgtcag
    10321 tctttcgaac cgctgcgtat tcagcgtctg gggcaggaac agcactatct gggaccgctt
    10381 aacggaccgg agctgctggt ggtgctaccg gaagcgaaag cggtgggatc ggtggcgatg
    10441 tcgatgctgg gaagcgatgc tgatttgggg gttgtgctgt ttaccagtcg cgatgccagt
    10501 cactatcaac aagggcaggg aacgcagtta cttcatgaaa ttgcgctgat gttgccggag
    10561 cttctggcgc gttggattga acgcgtatga ccgatttaca caccgatgta gaacgctacc
    10621 tacgttatct gagcgtggag cgccagctta gcccgataac cctgcttaac taccagcgac
    10681 agcttgaggc gatcatccat tttgccagcg aaaacggcct gcaaagctgg cagcaatgtg
    10741 atgtgacgat ggtgcgtaat tttgctgtac gcagtcgccg taaagggctg ggagctgcaa
    10801 gtctggcgtt acggctttct gcgctacgta gcttttttga ctggctggtc agccagaatg
    10861 aactcaaagc taacccggcg aaaggtgttt cggcaccgaa agcgccgcgt catctgccga
    10921 aaaacatcga cgtcgatgat atgaatcggc tgctggatat tgatatcaac gatcccctcg
    10981 ccgtgcgcga tcgggcaatg ctggaagtga tgtacggcgc gggtctgcgt ctttccgagc
    11041 tggtggggct cgatatcaaa cacctcgacc tggagtctgg cgaagtgtgg gtgatgggaa
    11101 aaggcagcaa agagcgccgc ctgccgattg gtcgcaacgc cgtggcgtgg attgagcact
    11161 ggcttgattt gcgcgacctg tttggtagcg aagacgacgc gctttttctg tcgaaactgg
    11221 gcaaacgcat ctccgcgcgt aatgtgcaga aacgctttgc cgaatggggc ataaaacaag
    11281 ggctgaataa tcacgttcac ccgcataaat tacgtcactc gttcgctacc catatgctgg
    11341 agtcgagcgg cgatcttcgt ggtgtgcagg agctgctggg tcatgccaac ctctccacca
    11401 cgcaaatcta tactcatctt gattttcaac accttgcctc ggtgtacgat gcggcgcatc
    11461 cacgcgccaa acgggggaaa taatgcgttt ttaccggcct ttggggcgca tctcggcgct
    11521 cacctttgac ctggatgata ccctttacga taaccgtccg gtgattttgc gcaccgagcg
    11581 agaggcgctt acctttgtgc aaaattatca tccggcgctg cgcagcttcc agaatgaaga
    11641 tctgcaacgc ctgcgccagg cggtacggga agcggaacct gagatttatc acgacgtgac
    11701 gcgctggcgt tttcgttcga ttgaacaagc gatgctcgac gccgggctga gtgccgaaga
    11761 agccagtgca ggcgcacatg cagcaatgat caactttgcc aaatggcgca gccggatcga
    11821 cgtcccgcag caaacccacg acaccttaaa acagctggcg aagaaatggc cgctggtggc
    11881 gatcaccaac ggtaacgccc agccggaact gtttggtttg ggggattatt ttgagtttgt
    11941 gctgcgcgct ggcccgcacg ggcgctcaaa accgttcagc gatatgtact tcctggcggc
    12001 ggaaaaactc aacgtgccga tcggcgagat cttacatgtc ggggacgatc tcaccactga
    12061 cgtgggtggg gcaattcgca gcggaatgca ggcctgttgg atcagaccgg aaaatggcga
    12121 tctgatgcaa acctgggaca gccgtttact gccgcatctg gaaatttccc ggttggcatc
    12181 tctgacctcg ctgatataat cagcaaatct gtatatatac ccagcttttt ggcggagggc
    12241 gttgcgcttc tccgcccaac ctatttttac gcggcggtgc caatggacgt ttcttacctg
    12301 ctcgacagcc ttaatgacaa acagcgcgaa gcggtggccg cgccacgcag caaccttctg
    12361 gtgctggcgg gcgcgggcag tggtaagacg cgcgtactgg tgcatcgtat cgcctggttg
    12421 atgagcgtgg aaaactgctc gccgtattcg attatggcgg tgacgtttac caacaaagcg
    12481 gcggcggaga tgcgtcatcg tatcgggcaa ctgatgggca ctagccaggg cggtatgtgg
    12541 gtcggcacct tccacgggct ggcgcaccgc ctgctgcgtg cgcaccatat ggacgccaac
    12601 ctgccgcagg atttccagat cctcgacagt gaagaccagc tacgtctgct taagcgtttg
    12661 atcaaggcga tgaatctcga cgagaagcag tggccgccgc gtcaggcaat gtggtacatc
    12721 aacagccaga aagatgaggg gctgcgtccg catcatattc agagctacgg taatccggtg
    12781 gagcagacct ggcagaaggt gtatcaggcg tatcaggaag cgtgtgaccg cgcgggcctg
    12841 gtggacttcg ccgagctgct gctacgtgct cacgagttgt ggcttaacaa gccgcatatc
    12901 cttcagcact atcgcgaacg gtttaccaat atcctggtgg acgaattcca ggataccaac
    12961 aacattcagt atgcgtggat ccgcctgctg gcgggcgata ccggcaaagt gatgatcgtt
    13021 ggcgatgacg atcagtcaat ctacggctgg cgcggagcgc aggtggagaa tattcagcgt
    13081 ttcctcaatg atttccccgg tgctgaaact atccgtctgg agcagaacta ccgctctacc
    13141 agcaatattc tgagcgccgc gaacgccctg attgaaaaca acaacgggcg tttgggtaaa
    13201 aaactgtgga ccgatggcgc ggacggtgag ccgatttccc tttattgcgc ttttaacgaa
    13261 ctcgacgaag cgcgttttgt ggttaatcgc atcaaaacct ggcaggacaa cggcggggcg
    13321 ctcgctgagt gcgccattct ctaccgcagc aacgcccagt cgcgtgtgct ggaagaggcg
    13381 ttattgcagg cgagtatgcc gtatcgtatt tacggcggga tgcgcttctt cgaacgccag
    13441 gaaatcaaag atgcgctctc gtatctgcgc ctgattgcca accgcaacga cgacgcggcc
    13501 tttgagcgtg tggtgaatac gccaacgcgg ggtattggtg accggacgct ggacgtggta
    13561 cgtcagacat cgcgagatcg ccagttaaca ctctggcagg catgtcgtga actgttgcag
    13621 gaaaaagccc tcgccggacg tgctgccagc gccttacagc ggtttatgga gctgatcgac
    13681 gccttagcgc aggaaactgc cgatatgccg ctgcatgtac agactgaccg ggtaattaaa
    13741 gactccggcc tgcgcaccat gtacgagcag gagaagggcg aaaaaggtca gacgcgtatc
    13801 gaaaacttag aggaactggt gacggcaacg cgccagttca gctacaacga agaagacgaa
    13861 gatttaatgc cgctgcaggc attcctctcc catgcggcgc tggaagcggg cgaggggcag
    13921 gcggatacct ggcaggacgc ggtgcagttg atgacgctac actcggcgaa aggtctggaa
    13981 ttcccgcagg tgtttatcgt tggtatggaa gagggcatgt tcccaagcca gatgtcgcta
    14041 gatgaaggcg ggcgtctgga agaagaacgc cgtctggcct acgttggcgt aacccgcgcg
    14101 atgcagaaac tgacgctgac ctacgcggaa acccgccgtc tatatggcaa agaggtttac
    14161 catcgcccgt cgcgctttat cggtgagttg ccggaagagt gtgtggaaga ggtgcgcctg
    14221 cgcgccacgg taagccgccc ggtcagccat cagcgtatgg ggacgccgat ggtcgagaac
    14281 gacagcggct acaagctcgg ccagcgcgta cgccacgcta agtttggtga aggcaccatc
    14341 gtcaatatgg aaggcagcgg cgagcatagc cgtttgcagg tggcattcca gggccagggt
    14401 attaaatggc tggtggcggc ttacgcccga ctggagacgg tgtaatttac gattccgttg
    14461 ccggatgcgg tgcgctacac cgcatccggc atcagcacct tacttagcgc acttcttcac
    14521 atcaatattc cagtcagcac cagacgcaac ctgatgtttc tgcgcgaaat tatccatatt
    14581 cagcgccacg gaaacattca ataagctgtt cagatacacc atcggctggc cttccggcac
    14641 atcgccgaag cttgcgacat acggcatttt ccctgcgtat ttttgctggg aaccttcgga
    14701 aatggtcaca cacaacgtgt cgttgagttt gattcctgcc tgattcagca actcgtcgct
    14761 gatattgctc cagacgttgc cgtactgaat atcgagaatc gggatattgc ccttcacttc
    14821 accttttgtt gctttggctt tctgataaga aagttcgaca acttttgcag gaagctctgg
    14881 ccccacctgc tcgaacgtga tcgccccgga agccagacgc gcaccggtgt aggcatatac
    14941 atcacgacca tggaaggtat atgacttttc ggaacctttc agacggttgg ttttttcatc
    15001 gatttgccgg acgctctcaa tacccagcga ttccgccacc aacgtgagcg taccgttatc
    15061 tggtgaaacg aagtactgac cgtttttggt tttcagcact accgatttac ggtctgtacc
    15121 aacacctgga tcgaccacgc taacaaacac cgaaccttgc ggccagtaac tggcagtctg
    15181 atacaggcga taagcgcctt cccagatgtt atacggtgga atttcgtgtg ttaaatcaaa
    15241 gattttaaga ttatggtcaa caccgaatgc gacccctttc atcgccgaga cggcaccatc
    15301 tttcaggcta aaatccgttt gtaaaatcaa agcgttatcc gcataagcgg atgatgaccc
    15361 cagacaggtt aataaaaata atgttgcgag cgtttttttc atttttgttt cccttcattt
    15421 atctgatgat tttccagctg tgtagccagc cccagtttgt ctggcctcct cgagtgtaca
    15481 atgtttaacc tttctgctcg acggaaatca tggtgacaaa aggatagcgt tgccacggga
    15541 ttgcctcgcc tttcatatac atatgtgaaa ttgtgccatc aagataaagc agctgctcaa
    15601 cgttcagttt cgctttggca taacaggcaa aatcataaaa atttgtcgcc tgctggctca
    15661 gcaaaaacac ggcgttccca tgtttattaa tcccaacacc gttacgaatt ttacgtgagg
    15721 cgacgttggg atgaatacgc ggattaatta caccgttttc catcaacaat ggccctgact
    15781 gcaccgcaaa ctgaatctct ttactggttt taaaggcatc cagacgaacg atgccgactt
    15841 tatctcccgc gacataaaac acgccgccag ggcgaataaa gaaattccct tcacctgaag
    15901 cgagatttaa cgccaccttc tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc
    15961 tttcatcata gatgccgccg ttcatcgcca tctgcacctg accctgacta ttaatatccg
    16021 ccagcagcgc atgtaacgtt ccccacgctt cgccattggc tttttgccag tacattttca
    16081 cccgctctgt ttgaggatta acggtatacg cctgtacggt cagcgtcgga tctgagggtg
    16141 cgcaatcatc agcggcaaca gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt
    16201 tgagattcaa ggtgatcatt cctttaccaa tgaatagccg atgcgccatt ataggccctg
    16261 aatgtgggat ttttttatcc tgttagcgac cttgacgagt accaaaaagc gcgaagttca
    16321 actattgttc tgtggtgttc tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc
    16381 gcgcacgatc actctaagag gacattcgcc ttggacacac ccagtagata ctggctcact
    16441 atcctgtcat ccaggatcaa ctcctaaggc tatccctttt tgctgatagc cttagcggtt
    16501 gtcagcgacc tcaatttttc ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca
    16561 atttgtttct gtgtgcccac cgaactgtcc gatattttaa gcattgggag tcccggtcat
    16621 gctgagcgca tttcaactgg aaaataaccg actgacccgg ctggaagtcg aagagtcaca
    16681 accccttgta aatgcagtat ggattgatct tgtcgaaccg gacgacgacg agcgactgcg
    16741 cgtacaatct gaacttggcc agagcctggc aacccgcccg gaactggaag acatcgaagc
    16801 atcggcacgt ttctttgaag acgatgacgg cctgcatatt cactccttct tcttctttga
    16861 agatgcggaa gatcacgccg gtaactccac tgtggcattt accatccgtg atggtcgtct
    16921 gtttactctg cgtgagcgtg aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag
    16981 ccagtcaatg gtggacggta acgcctacga gctgctgctg gatctgttcg aaaccaaaat
    17041 cgaacagttg gcagatgaaa ttgaaaatat ctacagcgac ctggagcagt tgagccgggt
    17101 aattatggaa gggcatcagg gcgatgagta cgacgaggcg ctctccacgc tggcggaact
    17161 ggaagatatc ggctggaaag ttcgcctgtg tctgatggat acccagcgcg cgctcaactt
    17221 cctggtacgt aaagcacgct taccgggtgg gcaactggag caggcgcgtg aaatcctgcg
    17281 agatatcgaa tccctgctgc cgcataacga atccctgttc cagaaggtga acttcctgat
    17341 gcaggcggcg atgggcttta tcaatatcga gcagaaccgc atcatcaaaa tcttctcggt
    17401 ggtatccgtg gtgttcctgc caccgacgct ggtcgcttcc agctacggga tgaactttga
    17461 gtttatgccg gaactgaagt ggagcttcgg ctacccaggc gcgattatct ttatgatcct
    17521 cgcgggcctg gcaccgtatc tgtactttaa gcggaagaac tggctgtaaa aaacgagaac
    17581 ggtggcttag cctggctaag ccaccgatta tttaaaggct ccaggtatga atacctttta
    17641 cttctttttc atagaaccat ctgttggtat tgtatgaaac ataagcggcg ataatgaatc
    17701 caaatacatt atagccaatc atgtaaccaa aaattaaaga aaatatcgcc aggaatagaa
    17761 caacaatagc cttcttccat aaacccaata caaataaata gacccacgag aagaaaaaag
    17821 caataacatt tgttctaatt attatctttt gccttgtgtt taatcctttt aatgcttgtt
    17881 tatattctgg gcttgcaccc caaaatccag ggaagccatg ttcatcataa aacgaaaagc
    17941 ggtacttcca cttttcagtt aatgagccat ctgccatata atccttactc ataaaatctc
    18001 cattaattat ttacgggggg aatcagtggg ggcaagaact aacatgccaa aggtggaaaa
    18061 ataacaaatc aaaaaaatca tcgaaccatt gccagaacag tcaaaatgct cagcaaccat
    18121 gatgatgaaa gaaatgatat agactactaa tgtac
//
LOCUS       DAPQZD010000092        18136 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPQZD010000092 DAPQZD010000000
VERSION     DAPQZD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.092"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            134..1120
                     /locus_tag="RO454_003042"
     CDS             134..1120
                     /locus_tag="RO454_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDY0934841.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGEEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEADQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            1165..1668
                     /locus_tag="RO454_003043"
     CDS             1165..1668
                     /locus_tag="RO454_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDY0934842.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            1668..2969
                     /locus_tag="RO454_003044"
     CDS             1668..2969
                     /locus_tag="RO454_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDY0934843.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLITLMMITYIPQISLFIPQLLGLM"
     gene            complement(3025..4032)
                     /locus_tag="RO454_003045"
     CDS             complement(3025..4032)
                     /locus_tag="RO454_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDY0934844.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVSIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(4149..5195)
                     /gene="ansB"
                     /locus_tag="RO454_003046"
     CDS             complement(4149..5195)
                     /gene="ansB"
                     /locus_tag="RO454_003046"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDY0934845.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(5371..6090)
                     /locus_tag="RO454_003047"
     CDS             complement(5371..6090)
                     /locus_tag="RO454_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDY0934846.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(6274..6600)
                     /locus_tag="RO454_003048"
     CDS             complement(6274..6600)
                     /locus_tag="RO454_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDY0934847.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            complement(6600..7319)
                     /gene="trmB"
                     /locus_tag="RO454_003049"
     CDS             complement(6600..7319)
                     /gene="trmB"
                     /locus_tag="RO454_003049"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDY0934848.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            7480..8532
                     /gene="mutY"
                     /locus_tag="RO454_003050"
     CDS             7480..8532
                     /gene="mutY"
                     /locus_tag="RO454_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDY0934849.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8560..8835
                     /locus_tag="RO454_003051"
     CDS             8560..8835
                     /locus_tag="RO454_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDY0934850.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            8900..9979
                     /gene="mltC"
                     /locus_tag="RO454_003052"
     CDS             8900..9979
                     /gene="mltC"
                     /locus_tag="RO454_003052"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDY0934851.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            10181..11437
                     /gene="nupG"
                     /locus_tag="RO454_003053"
     CDS             10181..11437
                     /gene="nupG"
                     /locus_tag="RO454_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDY0934852.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11486..13621)
                     /gene="speC"
                     /locus_tag="RO454_003054"
     CDS             complement(11486..13621)
                     /gene="speC"
                     /locus_tag="RO454_003054"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDY0934853.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFCVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            14014..14721
                     /locus_tag="RO454_003055"
     CDS             14014..14721
                     /locus_tag="RO454_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDY0934854.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14827..14902
                     /locus_tag="RO454_003056"
     tRNA            14827..14902
                     /locus_tag="RO454_003056"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14860..14862,aa:Phe,seq:gaa)
     gene            15100..16365
                     /locus_tag="RO454_003057"
     CDS             15100..16365
                     /locus_tag="RO454_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDY0934855.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            16621..17664
                     /locus_tag="RO454_003058"
     CDS             16621..17664
                     /locus_tag="RO454_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDY0934856.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWILPF
                     SAAGMKHTGIQYQ"
BASE COUNT         4505 a         4546 c         4301 g         4784 t
ORIGIN      
        1 aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac aggttaacca
       61 atttgtcgca caaagcactg ataagcacaa ttagatatca accaatttcc attcttcaac
      121 agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg catccttgct
      181 ggctctggtt agcactggcg taaacgccgc agaaaaagtg acgttaaagt tagcacacaa
      241 cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag aggtaaaaca
      301 gctgtcaaaa ggtaatatgg tgatccgtat ttatcccagc agccagatgg gtaatgcgcg
      361 agaaactatg gagttgttgc aaaatggtgc actggatatg accaaaggtt ccgccagtga
      421 tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat ttaaagatca
      481 ggcgcacttc aacaaagtgg tttttggtga agttggtgaa gaaattatgg attcaacaaa
      541 agataaaggc ttttttgctt tatcagctta tgtagcgggt acacgcagtt tctatgcgaa
      601 aaaaccgatt actaagccag aagatcttaa agggctgaaa attcgtgttc agccaagccc
      661 gaccaccatc aaaatgattg aactgatggg tggctcaccc actccaattt catttggcga
      721 agtttatact gcgatgcagc aaggtgtagt agatggcgca gaaaataacg tgccttcctg
      781 ggtgcagact agacatatcg aaattgccaa agtcttttct gaagatgaac atgcctcaat
      841 tcctgacttt ttggttatct ccaccaaaac atggaataaa ttaaccccag aacagcaaca
      901 aatcctcgaa acagcggcga agaaatccga agctgatcaa caaaaattgt gggaaaaaat
      961 tgacgctgac acacgtgctc aggcgaaagc tatgggtggt gaaatcgtta aggttgataa
     1021 agccccattc cgtgccgcag ttcaacctct gtttgacgac ttcaaaaaag atccaaaaca
     1081 agctgctttg ttagagaaat tcgacaacgc tgctcaataa tatttcgggc cagtctctgg
     1141 ccctgatttt ccaggatgct tacaatgata tttaaccgcc tgaaacttgc ggtagaccgc
     1201 gtcattgccg cgttttctgt cgccgtaatg cttgctctgg ttgtatgcgt agtctggcaa
     1261 gtatttagcc gctacgtact taaccagcca agtacattaa ccgatgagct ggcacgcttt
     1321 ttgatgatct gggtaggatt attaggtgct gcatataccg tcggcgcgca gagacatctc
     1381 tctatcgatt tatttgctct cgccctgaat aagcgcaagc aattacttct tagtatagtc
     1441 attaacgttc ttattttagg ttttgccggt tcggttattg tgactggtgg cctgaaatta
     1501 attgataaaa cactggcaac atcacaggta tctgccgcga tgcaaatacc gatgggatac
     1561 gtatatatca ttctgccatt aagtggattg gtgatgatgt tttatgcctt gtgttttatt
     1621 aatcaaagca ttcaacaact gaaacaacct gcgcaggagg ccagttaatg gatagttata
     1681 tcgcgcttac actgtttggc tgtttttttg tgctggtctt tattggtgtg cccatttcgt
     1741 tctcaatcgg tattgccact gtagcttcaa tgctgttgat gtttccctgg gatatcgcgg
     1801 caatcactgt ttcgcagcga ctggctaatg gactggataa ctttgcccta ttggcaatcc
     1861 cctttttcat ctttgccgga acgctgatga acagcggcgg tattgcgata cgtttgatta
     1921 acctcgccca ggtaatggtt ggccgcgttc ctggttcgct tggtcatgtg aacgtcctgg
     1981 cgaatatgat gtttggttcc atttctggtt cagctgttgc ggcagctgct gccgttggtg
     2041 gcacactgaa tcccattcag accaaagaag gttatgatcc ggcattttct accgctgtaa
     2101 acgtctcatc ttgtattact ggattactga taccgccctc aaacgtgtta attgtgtttt
     2161 ccctgacagc tggcggcgtt tctgtagcat cgctatttat ggcgggatat ttgccaggga
     2221 tcttaatggg tctcgcggta atgatagtct gcggcattat tgctaaacgc cgtggctacc
     2281 cactttcaga aagagcaacc tttgctcagg catgtaaagc gtttctggat gccttaccaa
     2341 gtttgcttct ggtgttcatt gtaatgggcg gcattctggg cggcattttt accgcaactg
     2401 aagcgtcagc aattgccgtt gtctacacat ttattctctc ggtgctgatt taccgggaag
     2461 taaaatggcg cgatttaccc aaactgattc tggaatcggt tgttaccact tcgattgtat
     2521 tactgttgat cggtttttcc gtgggcatgt cctgggcaat gaccaatgct gatattcctt
     2581 atatgatcag cgacgcgttg atggggattt ctgataaccc actggtcata ttgctgctga
     2641 ttaatatcgt cttattaatt gtcggcatct ttatggacat gacaccggct gtactaatct
     2701 tcacaccaat cttcttgccg attgcacaag agctgggaat ggatccggta catttcggta
     2761 ttatgatggt ggccaacctt tgtattgggc tgttaacacc gccggtgggt agtgcgctat
     2821 ttgttggttg ttctatttca ggtgtgaaga ttcaacattt gatcaaacca ttgctgccat
     2881 tttatgccgc attgttaatc acgttgatga tgatcactta catcccgcaa atatccttgt
     2941 tcattccaca gctactaggg ctaatgtaaa aaataatttg ccggataact ttttatatta
     3001 tccggcaaag gtacttaaca tgagttactt aatcactcca ttgcgccgca attgttcggt
     3061 atcttttttc cagcgttcat tagcctgatt gatatcgtca attttcatca aaatcgcgac
     3121 ttttttactg tttatttctg aaactttcat cgttaacgga tattgccctt tctcgagcgt
     3181 tatccagtca tcctgcaatt tacggctaag tttttgtgat tgcagaaaag tctgtcgccg
     3241 ttgttcgttg atatcgaaat ctcgcttctg ctgaatcgac acttcataac actcttcact
     3301 ctgtttatga catgccgcaa tcgccggttg ataaacctct ttgtcatatt taaccgcgaa
     3361 cgcctcgcta tcattctgtt tattaaactc atcacgctgt tggtgaattt ttttaaaggc
     3421 gtcttcgcgg gtcatttgct taccgtttgc atcttttccc caatatgcat ccgctgcttt
     3481 ctgcgccgcc accatttgct tatcaagcgc tttcacttcc ttttccagcg caggaatcga
     3541 gttcttctgg ttattcaacg cagactcaag ctgactaaag cgataattga aatcttcgct
     3601 attcacgatc agatatttat tattcgtttt gatatcgtcg accacccgcc cacgatcgct
     3661 ggctgccgcc tgaaaagagt aaaagttcac cgaccagcca gacgcgggcg ttcctttact
     3721 ggttaacatc gccgagaatt ttaccggttt atctttcgtc caggtctgtt cgagcagttc
     3781 gtaatctgcc aactgaccga cccaggtata gagatcgtca ctggaagaga catcgccttc
     3841 cgctgaccag gtggcctgat tacccttagc atcaagattt tttaaggtga tagcatcaag
     3901 cttgagaatg ccgtgatact gctttttgaa ttgatctttt aagatatttt cagttggcgg
     3961 ttcgccatct gcccagacat taccagtcat caacataagc agcgccgtgc caacaatcca
     4021 ttgctttttc acccgaaaac cctcgcgcgg aataatttat tccagtgatt atcatcaggg
     4081 taatgaaaat tggcaatgga cgtaagtctg aagtgaaaaa gccccggcac gataccgggg
     4141 cgaggtgatt agtactgatt gaagatctgc tggatctgct gcggatcttt ggtttgcgtc
     4201 agagccagct gcagcaggac gcgcgctttt tgcgggttca gcgtgccaga ggcgacgaag
     4261 ccgtatttcg catcatccac ttcagcatcc tgagtggtag cgcccgtcgg tacacgggaa
     4321 gaacgcacta ctgcagtgcc gtttttcgcg gcggttgcaa gggtgtcaaa tacggtttta
     4381 tacaggttgc cgttacccac accagcgctg acgatgccat catagcccgc atctaccagt
     4441 gctttagccg gaagatcgga tgcgttagcg tagttataaa cgatgccgac tttcggcagc
     4501 tcattcagct tagagacatc gaatggcgta tcgctggtgt gcttacgtgc cggggtacgt
     4561 tggtagtcaa tcttaccgtt gtgaatgtaa cccagaggac cgtagttaac agacttgaag
     4621 gtcgctacgt cggtggtgtt ggttttggtg acatcgcgac cgtccagtac ggtgtcgttc
     4681 atcaccacca gtacgccacg attagcggag gctttatcag ctgcggtcac taccgcgtta
     4741 tacaggttga atggaccgtc tgcgctcata gacgtggacg ggcgcattgc accgaccatc
     4801 accaccggtt tgtcgcattt caccgtcagg tcgaggaagt aagcggtttc ttccatcgtg
     4861 tcggtaccgt gagtaatgac gaagccgtcg gttttatcgc agtcggtgtt aatttttttc
     4921 gccagtgtca gccagacatt atcgttcatg tcctgggagc cgatattcac tacctgctcg
     4981 cctttaacgt tcgcaatgtc cttcagttgc ggcaccgcat taaccagatt ttctacgcca
     5041 actttacccg ctgtgtagtt agatttggtt gcggagtcac caccaccggc aatggtcccg
     5101 ccggttgcta aaatggtgat attgggtaat gccaatgctg caccactaaa acccataacc
     5161 agtgcggcaa gtgccgtctt tttgaaaaac tccatttcat tcctccagtt acgtgaacgc
     5221 tacgcattat cccttagctt tgtatgggaa atttgacgtt aaacaattta caacgtgaat
     5281 atattttgga gatctacaaa gttagaggca ggtaacaaaa cgaagaatta aacggcataa
     5341 aaaagtatta tgccgtctta aaatagagga ttattttaaa ttcccgacca gggctttgcg
     5401 actatcttcc agagtcacaa cgcggctaca aacatctttg ccaaactgct ggaaatcttt
     5461 ttcctgcttt ttccattcgg tttggattga ggattgcaga ccgcccaggc ttcccagcac
     5521 attctgtaat gggttaccac cgcttttcag caccgctttc gcgcccattt cattaatgct
     5581 gtcctgtaaa attccgccca ttgcctgatt cactaattgc tggccttcgg cgcgaacctg
     5641 atcaatggct ttatagtgaa acgtcaggcc atcgctgcgc gtttcgataa tgcggttcat
     5701 ctgctctttc agctgcgcat caagtttggt cagacggctg cgcattttgc tgctttcgcc
     5761 catctcctga acgataattt tatccagcgc aatacgggct ttctcgacgc ggcttttcgc
     5821 gccttcatca atccacggca gggtgctacg cagttcagcc tgataatcct tcgcctgctc
     5881 gcgctgggcg gcgttcaggg aatattgctt accgttatac atcacgttgc cgtctggcgt
     5941 gatcaccaga ttgccgtttt cgcccttcac ctgcacggtt tgcgggctga caatcacatc
     6001 gtcacgcggc gtgacgctgc actggtagtc ggcgtgagcg gtcattgccg tcactgaaag
     6061 tgctgccgcc agcagcattt tgcgcatcat agtcttccct caagaacaaa tcaggccagc
     6121 atttgctggc cccagattga taacaaagta catgtcatcc atgccggatg cggcgtaaac
     6181 gccttatccg gcctactcga ttgtgcatct ttccaggcct gataagcgta gcgcatcaga
     6241 cagttttgca tttgtcatcg cccgtatgtt ttcttagtcc caccaaacgt cgaaaagttc
     6301 gctggtgcgt acctcgccca gtttgcgctc ttccagccac ttacgcacaa tcgcctgatg
     6361 ttcttcggtg catttgccga tttcctgcat gcagatcaga ccttcccagg ccagataacc
     6421 gctgccgtca aaggccagtt tgttcggttc gataacctcg ttaataaagt catcaacggt
     6481 tttatcaatc tgttcttccg atgtaccttc cgggaatcgc catgccaccg aaaatcctaa
     6541 ttcctggaat tcgtcgatgt gcattttttt acgcagacga cggctacggt tctttgccat
     6601 tatttcaccc tctcgaacat taagtcccat actccgtgac caagacgatg accacgttgt
     6661 tcaaatttcg tcaccggacg tgatgccgga cgcggtacgt aatcattgct ctctgacagg
     6721 tttttataac cgtcaataga agacatcact tcaagcatat gttccgcata aggttcccag
     6781 tcggtcgcca tatggaatac gccccccagc tgcagtttgc tttttaccag ttcggcaaac
     6841 ggcacctgaa cgatacggcg tttattatgg cgcgctttgt gccacgggtc agggaaaaag
     6901 agctgcacca tgcgcaatga attgtcagga atcattttat gcagcacttc aaccgcatcg
     6961 tgacacatca cgcgcaggtt gcttaagccc tcttcatgcg cagaagccag gcacgcacca
     7021 acgcccggtg aatgcacttc aatgccgagg aagtcctgct cggggcgatc tttagccatt
     7081 gccaccagcg acgcccccat gccgaaacca atctcaagcg tcaccggcgc ttcacggcca
     7141 aaaagcgcag ggaaatccag catatcttcg ctgaactcaa cgcccatcac cggccagtag
     7201 ttttccagcg catgttcctg gcctttggtc agtcgcccct ggcggcgcac aaaactacgg
     7261 atacggcgca gtgggcggcc gttttcatca aattccggtg agatgacgtc gtttttcata
     7321 aagatttagt cgcttgtgaa agtgttctga aaacgggcat tatccaaagt tagttgccgg
     7381 atgcaagcat gataaggccg tggctgcgga aagttccggt ttacaccctg ccgtcgctgt
     7441 gctgcaatct tgcccccaac aacagtgaat tcggtgacca tgcaagcgtc gcaattttca
     7501 gcccaggttc tggactggta cgataaatac gggcggaaaa cgctgccctg gcaaattgac
     7561 aagacgccct acaaagtatg gctctcagaa gtgatgttgc aacaaactca ggttgcgacc
     7621 gttatcccct attttgaacg ctttatggcg cgcttcccaa cggtgaccga tctcgccaat
     7681 gcgccgctcg acgaagttct ccacttgtgg accgggcttg gctattacgc ccgcgcgcgc
     7741 aatctgcata aagcggcaca gcaagtggcg accttacacg gcggtaaatt cccggaaacc
     7801 tttgaagaag tcgcggcgtt accgggcgtc gggcgttcca ccgcaggcgc gattctctcg
     7861 ctttctctgg gtaagcactt tccgattctc gacggtaacg tcaaacgggt gctggcgcgc
     7921 tgctatgctg taagcggctg gcccgggaaa aaagaggtcg agaataaatt atggagtttg
     7981 agcgagcagg tgacgcccgc tgtcggcgtg gaacggttta atcaggcgat gatggattta
     8041 ggcgcgatga tttgtacgcg ctcgaagccg aaatgttcgc tctgtccgct acaaaacgga
     8101 tgtattgcca ccgccaacaa tagctggtcg ctttatccgg gcaaaaaacc gaaacagacg
     8161 ctgccggagc gcaccggcta ctttctgctg ttacagcacg aagatgaagt attgctggcg
     8221 cagcgcccgc cgagtggatt gtggggcggt ttatactgtt tcccacagtt tgccgacgaa
     8281 gaaagtttgc ggcagtggct ggcgcaacgg cagattgctg ccgataacct gacgcaactg
     8341 accgcgtttc ggcatacctt cagccatttc cacttagata ttgtgcctat gtggcttccc
     8401 gtgtcgtcat tcaccggctg catggatgaa gacaatgcgc tctggtataa cttagcgcaa
     8461 ccgccgtcag ttggcctggc ggctcccgtg gagcgtttgt tacagcagtt acgcactggc
     8521 gcgccggttt agcgcgtgag tcgataaaga ggatgattta tgagcagaac gattttttgt
     8581 actttcctgc aacgtgaagc agaaggtcag gattttcagc tgtaccccgg cgagctggga
     8641 aaacgcatct ataacgagat ctccaaagaa gcctgggcgc agtggcagca caaacaaacc
     8701 atgctgatta atgaaaagaa actcaacatg atgaatgccg agcaccgcaa gctgctggag
     8761 caggagatgg tcaacttcct gttcgagggt aaagaggtgc atatcgaggg ctatacgccg
     8821 gaagataaaa aataaaaaca gtgccggagc acgcctccgg caacttgcat aaaaacaaac
     8881 acaacacgca cccggaatga tgaaaaaata tctcgcgctg gctttgattg cgccgttgct
     8941 catctcctgt tcgacgacca aaaaaggcga tacctataac gaagcctggg tcaaagatac
     9001 caacggtttt gatattctga tggggcaatt tgcccacaat attgagaaca tctggggctt
     9061 caaagaggtg gtgatcgccg gtcctaagga ctacgtgaaa tacaccgatc aatatcagac
     9121 ccgcagccac atcaacttcg atgacggtac gattactatc gaaaccatcg ccgggacaga
     9181 acctgccgcg catttgcgcc gggcaataat caaaacgttg ctgatggggg acgatccgag
     9241 ttcggtcgat ctctattccg acgttgatga tattacgatt tcgaaagaac ctttccttta
     9301 cggtcaggtg gtggacaaca ccgggcagcc gattcgctgg gaaggtcgcg cgagcaactt
     9361 cgcggattat ctgctgaaaa accgtctgaa gagccgcagc aacgggctgc gtatcatcta
     9421 cagcgtcacc attaacatgg tgccgaacca ccttgataaa cgtgcgcaca aatatctcgg
     9481 tatggtccgc caggcgtcac gcaaatatgg cgttgatgag tcgctgattc tggcaattat
     9541 gcagactgaa tcttccttta acccgtatgc ggtcagccgt tccgatgcgc tgggattaat
     9601 gcaggtggta caacataccg ccgggaaaga tgtgttccgc tcgcagggga aatccggcac
     9661 gccgagccgc agtttcttgt ttgatcctgc cagcaatatt gataccggca ccgcgtatct
     9721 ggcgatgctg aacaacgttt atctcggcgg aattgataac ccaacatcgc ggcgttatgc
     9781 cgtcatcacc gcctataacg gcggcgcagg cagcgtgcta cgagtctttt cgaatgataa
     9841 gattcaggct gccaatatta ttaacaccat gacgccgggc gatgtttatc agacgctgac
     9901 gacccgccat ccctctgcgg aatctcgccg ttatctttat aaagtgaata ccgcgcaaaa
     9961 atcttaccgc cgccgataat tccactaacc gcccctgacg atgctcaggg gtaaaaatgt
    10021 tatccacatc acaatttcgt tttgcaaatt gggaatgttt gcaattattt gccacaggta
    10081 acaaaaaacc agtccgcgaa gttgatagaa tcgcatcatc tcgcacggtc aaatgtgctt
    10141 tttcaaacac tcatccgcat cacgatgtga ggaaattaac atgaatctta agctgcagct
    10201 gaaaatcctc tcttttctgc agttctgtct gtggggaagt tggctgacga ccctcggctc
    10261 ctatatgttt gttaccctga agtttgacgg tgcttctatc ggcgcagttt atagctcact
    10321 gggtatcgcc gcggtcttta tgcctgcgct gctggggatt gtggccgaca aatggttaag
    10381 tgcgaaatgg gtctatgcca tttgccacac cattggcgct atcacgctgt tcatggcggc
    10441 agaggtcact acgccggagg cgatgttcct tgtgatattg attaactcgt ttgcttatat
    10501 gccaacgctt gggttaatca acaccatctc ttactatcgc ctgcaaaatg ccgggatgga
    10561 tatcgttact gacttcccgc caatccgtat ctggggcacc atcggcttta tcatggcaat
    10621 gtgggtggtg agcctgtccg gcttcgaatt aagccacatg cagctgtata ttggcgcagc
    10681 tctttccgcc gttctggttc tgtttaccct gactctgccg catattccgg ttgctaaaca
    10741 gcaagcgaat cagagctgga caaccctgct gggcctcgat gcattcgcgc tgtttaaaaa
    10801 caagcgtatg gcaatcttct tcatcttctc aatgctgctg ggcgcggaac tgcagattac
    10861 caacatgttc ggtaatacct tcctgcacag cttcgacaaa gatccgatgt ttgccagcag
    10921 cttcatcgtg cagcatgcgt caatcatcat gtcgatttcg cagatctctg aaaccctgtt
    10981 cattctgacc atcccgttct tcttaagccg ctacggtatt aagaacgtaa tgatgatcag
    11041 tattgtagcg tggatcctgc gttttgctct gtttgcttac ggcgacccga ctccgttcgg
    11101 tactgtactg ctggtactgt cgatgatcgt ttacggttgc gcattcgact tcttcaacat
    11161 ctctggttcg gtgtttgtcg aaaaagaagt tagtccggca attcgcgcca gcgcgcaggg
    11221 gatgttcctg atgatgacca acggcttcgg ctgtatcctc ggcggcatcg tgagcggtaa
    11281 agtggttgag atgtacaccc aaaacggcat taccgactgg cagaccgtat ggctgatttt
    11341 cgcaggttac tccgtggttc tggccttcgc gttcatggcg atgttcaaat ataaacacgt
    11401 tcgtgtcccg acaggtacac agacggttag ccactaatta cgcaaagaaa aacgggtcgc
    11461 cagaaggtga cccgtttttt tattcttact tcaacacata accgtacaac cgtttcatgc
    11521 cattcgcatc ggtttcgcta tagacacctt gcaactccgg cgaaaatccc ggcaacaaat
    11581 tcacgccttc ttccagcgca aggaaataac gttgaaccgc cccaccccag acttccccgg
    11641 gtaccacgca aagcacgcca ggtggataag gcaacgcccc ttctgccgca attcgccctt
    11701 cggcatcacg aatccgcacc aactccactt caccgcgaat ataagcgcta tgcgcatcct
    11761 ggggattcat caccactgac gggaaactct gctggcggaa catcgctttt tgtaggtctt
    11821 tgacgtcgaa actgacatac agatcgtgca tctcctgaca caactgacgc agggtgtagt
    11881 cgcgatagcg caccggatat ttgttataaa cgctcggcaa cacctcagcc agcggcgagt
    11941 catcctcaat atgctgttca aactgcgcca gcatcgccac cagttgtgcc agcttctcgt
    12001 ggctttccgc cggagttaat aagaacagaa tggagttgag atcgcacttc tccggcacaa
    12061 tgccgttctc acgcaggtag tgcgccagaa tcgtcgctgg aacgcaaaaa tcgctatatt
    12121 cgccggtttc ggcatcgata cccggcgtgg tgagtaacag cttgcacgga tcaacaaaat
    12181 actgatccgc ggcatatcct tcaaagccgt gccacttcgc ccccggctca aaactgaaaa
    12241 aacggcggtc gctggctaac actgacgtcg gataatcctg ccacaatttg ccatcaacaa
    12301 cgggcgggat aaacgggcgg aacagcttac agcgcgcaag aatcgccttc cgggcttcaa
    12361 tccccaacgc aacgcactcc gcccacagcc gacgcccact ctccccttca tgaattttgg
    12421 cgttaacgtc cagcgcggcg aacagcggat agaacgggct ggtagaagca tggagcataa
    12481 aggcattatt caaccgctta tgcgggcaaa aacgcgcctg tccacggata tggttatctt
    12541 ttttatggat ctgcgacgtc tgtgagaatc ccgcctgctg tttgtgcacc gactgggtca
    12601 caaagatccc cggatcgttt tcgttaagtt ctaacagcag cggcgagcta tccgccatca
    12661 tcgggataaa ctgttcgtaa ccgacccacg cggaatcaaa cagaatgtaa tcacacagat
    12721 gcccaacggt atcgatcacc tgacgggcgt tatagacagt gccgtcatag gttcccagct
    12781 gaataatcgc caggcgaaac gggcgcggca ggtcggcttt ttctggcgca acgtcgcgaa
    12841 tttgctggcg cagatactct tcattaaaac agtgcgcatc aataccgcca atgaaaccaa
    12901 acgggttacg tgaagcttcc agatagaccg gcgtcgcccc cgcctgaatc aacgcgccgt
    12961 gatgattcga cttatggttg ttacggtcga agagcaccaa atcgccacgc gttaacagcg
    13021 cattcgtcac cactttattc gctgccgatg tgccgttcag cacaaaatag gttttatcgg
    13081 catgaaagac tttggctgcg aatttctgcg catctttcgc cgatccttca tgaatcagca
    13141 gatcgcccaa ttttacgtca gcgttacaca tatcggcgcg aaagatgttc tcgccaaaga
    13201 aatcgtaaaa atggcgtccg gcaggatgct ttttaaagaa cgcaccatgt tgatgtccag
    13261 ggcaagcaaa ggtgctatta cccatctcaa cgtactgcgt cagcgtgtca taaaacggtg
    13321 gcagcaaatt ctcttcatac tgacaggctg cggattccag ctccagccac tgctgctcgt
    13381 tgccgttgat taccgccgta acgcccgcag gtaattcaac aacatgttcg gaatacaaaa
    13441 acaccggtag atgaaaaccg gtgcgcttaa gcaacgcaag aatgccactg cgactatccg
    13501 cagcggtaat gacgactgcc gcgacgtccg taaaatcagt atctgccaac gccaccacgc
    13561 ggcgatgagt agaaagtcgg gataccagtt cactactggc ggcaatattc attgatttca
    13621 taagcgcaaa acccgtttcg gggaagtaag aataccggac aaggtgaaaa accctgcccc
    13681 acgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
    13741 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagaa
    13801 aaagggagga gtttcgcgcg aaacggcgat aagcgagaga atgtaaggag gtggcgtgca
    13861 tcgggcaaac tccgtacaga gaggagaaaa ttcgcgcaat catggcacct ttcgctaaag
    13921 cgtgcaagcc aggactttgc gaccagacaa gccatcaaca aggcaaaaag ttcataataa
    13981 gaaaatcaaa caatacagtt aacaggaact cttgtggtca tcggcccttt tatcaacgct
    14041 agtgcagtct tactgggtgg cgttctcggc gcactgctca gccaacgctt accggaacgt
    14101 atccgcgtct ccatgacatc aatttttggt ctggcatcgc tggggattgg tattttactg
    14161 gtggtgaaat gcgccaacct tccggcgatg gttttagcaa ccttactcgg tgcattaatc
    14221 ggcgaaattt gtctgctgga aaaaggcgtc aataccgcgg tcaccaaagc acaaaatctg
    14281 tttcgccact cacgtaagaa gccagcgcat gaatctttta ttcagaatta tgtcgcgatt
    14341 attgtcctgt tttgcgccag cggcaccggg atcttcgggg cgatgaacga agggatgacc
    14401 ggcgatccga gtattttaat cgccaagtca tttcttgatt tctttacggc gatgatcttc
    14461 gcctgctcgc tgggtattgc ggtatcggtg attagtatcc cattactgat catccagtta
    14521 acgctggcgt gggctgccgc gctgatatta ccgctgacca caccgtcgat gatggcagac
    14581 ttcagcgccg taggcggttt attgctgctg gcaaccggat tacgcatctg tggcattaaa
    14641 atgttcccgg tggtcaacat gcttccagca ctccttctgg caatgcccct ttccgccgcc
    14701 tggaccgcct ggtttgcctg acaatgcgtg cattagcggc aaagtgatga tagattgtgc
    14761 agtctgcagt aaattgaaga aatttgattg acgagacgag gcgaatcagg tttaatgcgc
    14821 cccgttgccc ggatagctca gtcggtagag caggggattg aaaatccccg tgtccttggt
    14881 tcgattccga gtccgggcac caagttcata tcaacggacc tccacggagg tccgtttttc
    14941 gtttcagaac gccacaattt aagcgttctg cctccaaatc aattctaccg aactcaacca
    15001 gattcccccc acatcaaccc cattgtgtgg gtataattgc gggtataccc cagttcgaca
    15061 gaatttgtac cctctttaac gcattcagaa ggctgaacta tggcactgac tgacgcaaaa
    15121 atccgggctg caaagcccac tgacaaggct tataaactca ctgacggggc tggcatgttc
    15181 ctgctggtac atcctaatgg ttcccgttac tggcgtctcc gttatcgtat tctgggtaag
    15241 gagaaaaccc tggcacttgg tgtgtatccg gaagtttctc tctccgaagc tcgtacaaaa
    15301 cgggatgagg cccgaaaact gatttcggag ggggttgacc cttgcgaaca gaaaagagct
    15361 aaaaaagtag tccctgattt acagctctct tttgaacata ttgcacgacg ctggcatgcc
    15421 agtaataaac aatgggcaca atcacacagc gataaagtac tcaaaagcct cgagactcac
    15481 gttttcccct ttatcggcaa ccgggatatc acaacactca gtaccccgga tctgcttatc
    15541 cctgttcgtg ctgcagaagt taaacaaatt tatgaaatcg ccagtcgtct gcagcaaaga
    15601 atatctgctg taatgcgtta tgccgtacag tctggcatca tcagatataa cccggctctg
    15661 gatatggctg gtgcattgac cacggtaaaa cgccagcatc gccctgctct ggatctttca
    15721 cgcctgcctg aacttctgtc gcgtattgac ggttataaag gccagcctgt cacccgactg
    15781 gcagttatgc tgaatttact ggtttttatt cgttccagtg aactcagata tgcccgctgg
    15841 tctgaaattg atattgacaa tgccatgtgg actattccag ccgaacgcga acctctgcca
    15901 ggcgtaaaat tttcataccg gggctccaag atgcgaacac cacatcttgt gccactcagc
    15961 caacaggctg tagccatact ggcagaactt cagacatggg caggtgaaaa tggtctgatc
    16021 tttacgggag cacacgaccc acgtaaacca atcagtgaaa ataccgtaaa taaggccctc
    16081 agagtgatgg ggtatgacac aaccaaggaa gtctgtggcc atggattccg ggcgatggcg
    16141 tgcagtgcat tgattgaatc cggcttatgg tcaagagatg cggttgaacg tcagatgagt
    16201 catcaggagc gcaacggagt acgtgccgcg tatattcata aagcagaaca tctggaagaa
    16261 cgtcggctta tgctgcaatg gtgggcagat tttctggatg caaacaggga ggagtgtatc
    16321 agtccgtttg aatatgcaaa agttaacaat ccactgaaaa ggtgactgaa cgccatttac
    16381 acacatgctc ttcctcttaa ggggaagagc acccgcctca atcaatttct tttttatgcc
    16441 ggcggaacca tcagccaggc tgaggttctc attcaataac tcaacgtctt accacagaac
    16501 agaattcact gtattatcat gggcaacgtt aatcccggac aataaaagga caccgatgcc
    16561 aggcatcaca atttttatag cctgcaggtt atggtccatc cttttttaca ggttcagaat
    16621 atgactggca aattacgctt tgaagtgaac gataaccagg gctgtttcat atttccggaa
    16681 acctggtttg gctcgttgct ggatgagttt gaagaactga ttgatgcata cgatgcggat
    16741 gaaatcagcg aaacaagtta catcaacaaa ctcaggcgcc ttgcccgaca ggagaatgac
    16801 tttattgatg tacacgctca cttggcatac gtatttctgg agcagaatgc cccgcgtaag
    16861 gcgctgaatg ctgcccttaa agggctggca attggcaatc gtcttatccc tgaaggtttc
    16921 agtggcagaa tcatctggat acatcctgac aaccgacctt ttcttcgtgc cctctatgcg
    16981 gcaatcctgg caaatgcaca tttacagcgt catcaggatg ccatcatgct cattgaaaaa
    17041 atactggatt acaatcccga agataatcat ggtgcacgct ggctactggg accggaactg
    17101 ttacgcactg gcgctcatga acaggcacgc catatactgc aggaacatgc agatgaattt
    17161 tcaccttact ggtatgaact cggccttctg cattttctta atggggaact ggtaaaagct
    17221 gcaactgcat ttcgtcgcgg ctttgcggca aatacttaca tcgcagaaat actgtgcgga
    17281 aatctgcatc catttccact tgctgtatgg cataactttt caggcggtcc ggatactgcc
    17341 gaagattatt atgccacata ccacccgtta tgggggcaat atccggaagc attgctgttt
    17401 gtaaactggc tctataatca ttcctctgtg ctgcatgaac gggcagaaat cattaaatgc
    17461 gccgaaatgc tgatgcagga agatgatttc gaaatatgcg aaagtatttt aagacagcag
    17521 gagaagttgc gtgaaagaat tgatgagacg ctttctgaga aaattgtaca gaaatgcaga
    17581 aatatgaatg gtgaatatat ctggccctgg atattgccgt tttcagcggc aggcatgaaa
    17641 catactggca tacagtatca gtagatattg cattagtgta tcctgcacac aagtaataat
    17701 ttattcacca ataataacac tgttaatgcc cccttcccct ggttgtcagc caggggttat
    17761 cttctgaata tttcttttga aaaggataac acagtaaatt atttttatga attatcccat
    17821 ggactcatta acaccctttc atgatgcttt attgtcaaac acgttatggc tgacatcaaa
    17881 aaaaaccgga tttcctctgc cagcgggtaa tcaccttccc ggtgttttcg gttggtctgg
    17941 ttactcctgt ctggttatta gcaagataat tgctataaac agtggaaaac tcatcgtaca
    18001 taatctggtg atgaacatta cgcttatttt cccttgaccg gaagaatcag aggctgtggt
    18061 ttcagactgt ctgccagtac attcctctct ccgttaaaaa ccataacggg ttcattatct
    18121 tcgtctgtca gcagat
//
LOCUS       DAPQZD010000093        17987 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPQZD010000093 DAPQZD010000000
VERSION     DAPQZD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.093"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="RO454_003059"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="RO454_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDY0934857.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /locus_tag="RO454_003060"
     CDS             complement(916..1482)
                     /locus_tag="RO454_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDY0934858.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="RO454_003061"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="RO454_003061"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDY0934859.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3484..3876)
                     /gene="flhe"
                     /locus_tag="RO454_003062"
     CDS             complement(3484..3876)
                     /gene="flhe"
                     /locus_tag="RO454_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDY0934860.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(3876..5954)
                     /gene="flhA"
                     /locus_tag="RO454_003063"
     CDS             complement(3876..5954)
                     /gene="flhA"
                     /locus_tag="RO454_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDY0934861.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5947..7095)
                     /gene="flhB"
                     /locus_tag="RO454_003064"
     CDS             complement(5947..7095)
                     /gene="flhB"
                     /locus_tag="RO454_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDY0934862.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7284..7928)
                     /gene="cheZ"
                     /locus_tag="RO454_003065"
     CDS             complement(7284..7928)
                     /gene="cheZ"
                     /locus_tag="RO454_003065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDY0934863.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVIDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQGQVDDLLDSLGF"
     gene            complement(7939..8328)
                     /gene="cheY"
                     /locus_tag="RO454_003066"
     CDS             complement(7939..8328)
                     /gene="cheY"
                     /locus_tag="RO454_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDY0934864.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8343..9392)
                     /gene="cheB"
                     /locus_tag="RO454_003067"
     CDS             complement(8343..9392)
                     /gene="cheB"
                     /locus_tag="RO454_003067"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDY0934865.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9395..10255)
                     /gene="cheR"
                     /locus_tag="RO454_003068"
     CDS             complement(9395..10255)
                     /gene="cheR"
                     /locus_tag="RO454_003068"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDY0934866.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10370..10500)
                     /locus_tag="RO454_003069"
                     /pseudo
     CDS             complement(10370..10500)
                     /locus_tag="RO454_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10546..12207)
                     /gene="tar"
                     /locus_tag="RO454_003070"
     CDS             complement(10546..12207)
                     /gene="tar"
                     /locus_tag="RO454_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDY0934867.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQSELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLAAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLAATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12352..12855)
                     /gene="cheW"
                     /locus_tag="RO454_003071"
     CDS             complement(12352..12855)
                     /gene="cheW"
                     /locus_tag="RO454_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDY0934868.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12876..14840)
                     /gene="cheA"
                     /locus_tag="RO454_003072"
     CDS             complement(12876..14840)
                     /gene="cheA"
                     /locus_tag="RO454_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDY0934869.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTSPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14845..15771)
                     /gene="motB"
                     /locus_tag="RO454_003073"
     CDS             complement(14845..15771)
                     /gene="motB"
                     /locus_tag="RO454_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDY0934870.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15768..16655)
                     /gene="motA"
                     /locus_tag="RO454_003074"
     CDS             complement(15768..16655)
                     /gene="motA"
                     /locus_tag="RO454_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDY0934871.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16782..17360)
                     /gene="flhC"
                     /locus_tag="RO454_003075"
     CDS             complement(16782..17360)
                     /gene="flhC"
                     /locus_tag="RO454_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDY0934872.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17363..17713)
                     /gene="flhD"
                     /locus_tag="RO454_003076"
     CDS             complement(17363..17713)
                     /gene="flhD"
                     /locus_tag="RO454_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDY0934873.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4289 a         4997 c         4428 g         4273 t
ORIGIN      
        1 ctagcacaat gctatgcgca ctgtcacccg ctcaacagat cacggcaatc gttcgttttt
       61 tatactactc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgacat aacgcgaata ctcgtccgcg
      241 tgtgcatctg atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttcc
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac ccgggcaatg
      481 cccaattccg taagattatt gagtgtattt aaagggttag cgcacatatc gaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggact
      661 gtgcgcacat cttcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgga taatcggatg cactgggatc gtcacccgct ggcgcacgga tttcagtacc
      781 cccaacgacg gcgttaggcc cccctctttt ggagctgcgc ataattcaac tctgtcagcg
      841 ccgttttgct gcgccgttag cgcgcattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttgctc cttagttacg ccgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atggttgttt taccatcggt gaccgccagc gtcggattcg gtaatcgttc
     1021 atgctcacct tttggcgaac tggttttaac tgagatcccc ggcaggctga gtccttcatc
     1081 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggta gaacgatttc
     1141 aatatgttcc caaccttcgt gcgggtaacg tttttccccg ggccacggta gctccacaat
     1201 ggaaaactgc cagtgcgcaa cctgtagcgg ttcatgcaat ttaaacaaac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccgcactgt tcaaacccgc gacgccagcg
     1321 ttcagcggtg acgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 atccaacccc agacggcggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcattc ttcgtctttc
     1501 attaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gtaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagttcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaggatgaag agtttgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccagatc acctacgatc gtctgaacgt gacgttgacc cgcgatgacg
     2461 tcatgggtga aagtctctac aacccaatgc tgccgggtat cgtggcggac ctgaaagcca
     2521 aaggtctggc agtagaaagt gaaggcgcga ccgttgtatt ccttgatgag ttcaaaaaca
     2581 aggaaggcga accgatgggc gttatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgcgcg aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt
     2701 attacatcga ttcccgtcag catcaacacc tgatgcaggc atgggcgatc gtccgtaaag
     2761 cgggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggca
     2821 aagatggtaa accgttcaaa acccgcgctg gcggtacggt gaaactggcc gatctgctgg
     2881 atgaagccct ggagcgtgca cgtcgtctgg tggcagaaaa gaacccggat atgccagccg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc cgtaaagcag
     3121 aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac tgtggttgcc cgtgaaggca
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggccgg tctgttctct ggcttctatg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac ggcgaagacg ctgaagctgg gtctggatac gctgggtatt gagacagtag
     3421 agcgtatgta atcgcgttat acggcaacag ccgatgtcat tgcagacatc ggctttttta
     3481 tcattaacga taatttacaa tcacttcatt ccgttgaatc tttagcggcg gagttagccg
     3541 cccaccgcca ggcacttccc agataaagcg caacggttct gccgccggta taccggaaaa
     3601 ggccatggtg gttccactct gcccctccaa ttcgacacaa cgagattgtg aacacaagcg
     3661 aacccgcagt cctgcaggtg tcggaccgat aagctgataa cgccacgcca ccagcgtcat
     3721 caatcctgaa gcaggttgtc gcgtagaaag aggcgctgac gacatcgatt caccgcgatg
     3781 atttagcgta acgcccacgc tactcgcctg ccacatcccc tccccggcgg cttgcaccag
     3841 cagcggaaac aataatattg ctaataaggc tctcattatt tgccgccaat cgtcgccgtc
     3901 atacggatat gtcggttatc tgatagttcc agattcgaca gcaccactaa ctgcggcaag
     3961 ctgcggcgca ggaagcgaga caataatggt cgtagcgcgt ggttcaccaa caataccggc
     4021 ggcgcaccca acatctcctg acgggatagc gcttcctgag tttgcgccag taaacgatcc
     4081 gccagccctg gctccagtcc accaccgcct tgcaacgcct gtagcaacaa acgttccagc
     4141 ggtgtatcga ggccaataac atggacttca tctttgccag gaaaccactg ctgggtaatc
     4201 gcccgtccca acgctacgcg cacgacagcg gttaattcat gcggatcgct ttggatgggc
     4261 gcatgttccg ccagcgtttc gagaatggtg cgcatatcgc gaatcggcac tttttcatcg
     4321 agcagatttt gcagcacttt atgcagtgtg gtaagcgtga cgacgccagg aacgagatct
     4381 tccgtcagct ttggcatctc ctgggcgacg cgatccagca gctgttgtgc ctcctgacga
     4441 ccaaacagct ctgcggcatg ctggctaatg aggtggttaa gatgcgttgc caccaccgtg
     4501 ctggcctcaa ccacggtgta cccctgaatc tgcgcctgtt ctttaagcgc actttcaatc
     4561 cagatagcat tcaggccaaa tgccggatca acggtcgctt caccaggtaa tgttccggcg
     4621 gcagtgcctg gattaattgc cagccagcgc cccggataag catcaccact ccccatttcg
     4681 acacctttca tcaagatacg atagcgggct ggttgcaaat ccatgttatc gcggatgtgc
     4741 accactgggg gtaggaagcc catctcctgg gcaaatttct tgcggatact gcgtatacgg
     4801 cccaacaact caccatcctg ctgaaaatca accatcggga tcaaccgata ccccacttcc
     4861 attcccagag aatcttccag ttgtacatcg ttccacgttg cttcgactac cgaattattc
     4921 tctgccattt ttaccggttt tggttcggca ggcgcttttt gttcacgtcc gcggatccac
     4981 caggccagcc ccagcaatcc ggcagtgaac agcaaaaata ccaggtttgg cattccaggc
     5041 accaggccga gtaaaccgag cacggcggcg cttaataaca taacgcttgg gttactgaaa
     5101 agctgattca ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt aacgatgacc
     5161 cccgcggcgg tagaaatcac caacgccgga atttgtgcca ccagaccgtc gccaatggtc
     5221 aatagcgtat aactttccgc cgcgtgcccc atgctcatgc catgttgcag cacaccgacc
     5281 agcaatccgc cgacaacgtt aatgaccatg atgaggatcc cggcgatggc atcgccgcga
     5341 acaaacttac ttgccccgtc catcgagccg taaaaatcgg cttcctgagt cacttcggag
     5401 cggcgttttt tcgcttcatc ttcaccaatc aatccggcgt taaggtcggc gtcaatcgcc
     5461 atctgcttac ccggcatacc atcgagaaca aagcgcgcgc ccacttccgc aatacgcccg
     5521 gcacctttgg tgataaccat aaagttgatg atcacgagaa tgacgaacac cacgataccg
     5581 atagcgaaat tgccaccgac gaggaagtga ccgaacgctt cgaccacctt tccagccgcc
     5641 gccgcgccgg tatgcccttc catcaaaatg atacgggttg aagccacgtt aagtgccaga
     5701 cgcaacagcg tggtaaacaa cagaatggtc ggaaacgcag caaactcgag cgtgcgctgg
     5761 gtaaacatcg ccaccagcaa caccatgatc gacaaggcaa tattgaaggt aaacagcagg
     5821 tcgagtatga atgcgggcag tggcagcacc atcatcgaca agatcaacag gatcaaaatc
     5881 ggtccggcaa ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg cagcatcgcg
     5941 gccagattac tcatgggtcg gtttctcgtt aataaaatcc agggcttccg gcaccggaag
     6001 atgagtaggt tgtacagggc gctgtccacc agccagacgc cagcgtttca gttgccagac
     6061 ccaggccagc acttccgcca ccgcggcgta cagttgaccc gggatttgtt gaccgatctc
     6121 cgcatgtcga tacagcgcac gcgccagcgg cggcgcttca agcgtcggga cgttattttc
     6181 agcgccaatt tcacgaatgc gcagcgcgac cagccctgca cctttagcga ccactttcgg
     6241 tgcgctcatt ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg ggttattgac
     6301 aatgacatcc gctttcggca catcggccat catccgacgc cgtgcggccg ctcgctgcat
     6361 ctgacggatc cgccctttaa catgggggtc gccttcgctt tgtttgaact catcacgaat
     6421 atcctgccgt gacattcgca gctttttcag gtggctgaag atttggaaaa agacgtcaaa
     6481 tcccaccatt ggaatgacac caagcaccac cagcagcgcg catagcccta ccagatccat
     6541 tgcattaccc atggcggtaa tcggagactc ggccattaag cgcatcatct gcggccagtg
     6601 ataccagaga aaaaaccccg tcacgctgcc aaccaggatg gttttcaaaa ttgccttaag
     6661 caactccgcg ccagtctgtg ccgagaacgt ccgtttaatg cccggtagcg ggttgagttt
     6721 ggaaaacttc ggctgcaagg attttccgct aaataccagc ccccccagca taaccggaga
     6781 aataatcgcc accagcacca cgccgctaat cagcggtagc agcgccagca tggcttctct
     6841 gatcagcaga ataatctgcc cgaggatcag attcgggtca ttgataatac tgtgatcaaa
     6901 atgtagccct gcggagagca tgcccgacaa tcgacgggcc agcgacacac cgccaaacca
     6961 gataacacta acgcccacca gcaaaatcag cagtgaagtc agttcacggg aacgcgggat
     7021 ttgcccctct tcccgcgctt tttctagtcg gtgaggtgtg ggggcctctg ttttgtcgtc
     7081 gctctcgtca gacacgtcgc caatcctgga aagagtatta agcgtaaatg atgccagagc
     7141 gcaaagcgtt caatggtttg agtaaggggc aaaacaggcg ggatttaggg cttttactgc
     7201 cacacatcaa gcatagtatg cgtttgtcgg atgcggcgtc atcgcctgat atgacgtggt
     7261 cacgtcacat caggcaatac aaatcaaaat ccaagactat ccaacaaatc gtccacctga
     7321 ccctgactgg ctaccacacc ggctttgctg gtatcgacct gaggtccatt aagcaaactc
     7381 tggttttcac gttttggacg cgactcctgt tccgggatgt tttccaacaa caccatcagc
     7441 aactgacgtt cgatctcctg aatgacatcc atcatccgct taatgacctg cccggtgaga
     7501 tcctgaaaat cctgcgccat catgatttcc agcagttgcg cgttagtaaa gctggtatgc
     7561 gcggggacat ctgccagaaa ttgccgtgta tcgatcacca gttcacgggc gtcggcaagg
     7621 tcaatcggat cggcaaacca gtcatcccaa cgttgggtta acgcttttgc tgatttctcc
     7681 atttgatcct gatgcggttg tgacgcctca acactgttca gcgcccgctc cgcagcctgg
     7741 gcggtcatct gcacaacata gtacaaacga tcgcgcgcat cggggatggc ttccgctgct
     7801 tcggcaatgg cctgatccag ccccagttcc cgcaaactgt cgcgcagcat acgcgtcagg
     7861 ctgccgatgc gcgcaatgat atcgccagct gaatgctcgt cagcaggttt gattgatggt
     7921 tgcatcatag tcgcatcctc acatgcccag tttctcaaag attttgttga gtttttcctc
     7981 cagcgtcgcg gcggtaaatg gcttcaccac atagccactg gcccccgctt gcgccgcagc
     8041 aatgatgttc tctttcttcg cttctgcagt caccattaac actggcaatg ccgacatcgc
     8101 gccatccgca cgaatcgttt tcagcaactc caggccatcc atattgggca tgttccagtc
     8161 ggagataaca aatccataac cgcctgcctg caacttattg agagcgtcga cgccatcttc
     8221 cgcttcctca acattattga atcccagctc tttcagcagg ttacgcacta tgcgtcgcat
     8281 ggtggaaaag tcatccacaa ccaaaaattt aagttcttta tccgccattt cacactcctg
     8341 atttaaatac gtatcgcctg tccggcacta atttttgcca gcatttgctg acttacctgg
     8401 ctaagatcga ccacttcgca gacaccaccc atattgatgg cctcgcgcgg catgccgaac
     8461 accacgcaac ttgcttcgtt ttgcgcaagg gtccatgccc ccgcctgacg catcgccaac
     8521 attcccgccg cgccgtcgtt gcccataccg gttaggatta ccccaaccgc attacgcccc
     8581 gcctgtttgg ccacagaatg gaacaacaca tctaccgatg gccgatggcg attaaccgcc
     8641 gggccatcgt gaattttgat ttgataattt gcgccactac gcgccagctc catatgccga
     8701 tcgcccggcg cgatataggc atgtcccggc aagacgcgct ctccatcttc ggcttcttta
     8761 accccgatct ggcaaagctt attaagtctg tcggcaaaag agcgggtgaa accgggcggc
     8821 atatgctggg taattaacag tgccggactg gaaagcggca acggttgcag tacgtgacga
     8881 attgcctcag ttccccccgt tgaagcacca atcgcaatca gtttttcaga actcaacaac
     8941 ggccccgcct tcagcgttgt cggtgccgac aatggcttat gtgctgcaag gctcgccttt
     9001 gctgccgtac gcaccttttc agcaatcatt tcgctatacg ccagcattcc ttcgcgaata
     9061 cccagttgcg gtttggtgac aaaatctatc gcccccaact ccagcgcgcg cagcgtgact
     9121 tctgaccctt tgccggtcag ggaagaaacc atcacaacgg gcattggacg caaacgcatt
     9181 aatttttcga ggaaatccag tccgtccatc cgcggcattt caacatccag cgtcaataca
     9241 tcgggattga atttcttaat caaatcacgc gcaaccagcg gatcaggggc ggtcgccacc
     9301 atttccatgt cgctatggct gttgatgatt tctgtcatga tctggcgcat cagtgccgaa
     9361 tcatcgacag ataacaccct gattttgctc atcgttaatc cttacttagc gcatacaccg
     9421 tctgaccacg cagcgtgaag cggcgctcaa ggtggctaaa gttttcagag tgacccgcaa
     9481 acagcaatcc gtcgggttta aggagcgaaa caaagcggcg caaaatctcc tgctgggtag
     9541 tttgatcgaa gtagatcatg acgttacgac agaagatcgc atcaaacggc cccggcacgg
     9601 tgtactgttt cgccagcaaa ttcagcgggg caaaatcaac atagttcgcc agctcctgac
     9661 gcacgcgtac cagcccttca tgcggtcccg tccctcgcat gaaataccgt tgcagttgct
     9721 gcggcgtcag gtttttcaac tcttcatggc gataaatacc gcttctggct ttttccagca
     9781 cttcggtgtc gatatcgctg gcaaacactt tccagcgtcc gggcgcggtg cccaatgtat
     9841 cagccagcgt catcgctatg ctgtacggct cttcgccggt tgaagccgcc gcgctccata
     9901 cgcgatactc gccagaacgg cgacgtgcgt gatccgcgag cagaggaaaa tggtgtgcct
     9961 cacggaaaaa tgccgtcaga ttcgtggtca gcgaattgat aaacgcctgc cactcaccgc
    10021 tgtgctgatt agattccagc aagttcaggt aatgaccgaa atccgccagt cccagcgcac
    10081 gcaaacgacg aaccagtcgg ttgtaaacca tgtcgcgttt atggtcagcc agaacgatcc
    10141 cggcgcgttg atagatcaat tgacttatcc gccgaaaatg cgcgtcggaa agcgccaggc
    10201 gctcggtcat ctgtaacaat aaagacgttt gcccacaggg cagagatgaa gtcatagcgc
    10261 cttctcaatc acttcaggat accactggcg caatttgtaa ctgcgccgac tcatgtcgtg
    10321 ctacttcatg ttcttcaagg gtaaataccg ccacacgcga cgaaagatgg tcggcctgat
    10381 tcagtcgttg caaatcgtcg cgaaatgccc aaaagacatg ccgttactgc caatctgcaa
    10441 gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat
    10501 caacgctccc catcaggcgg caatgaccgc tttagtaaat actcgtcaaa atgtttccca
    10561 gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg
    10621 acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga acgcggaaac
    10681 cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag ctgattcctg
    10741 caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg
    10801 atcgatgcca cgactctgtt catccgatgc tgatgcaatc tcgcccataa tgtcagtcac
    10861 gcgagtgaca gcattgacga tattgttcat tgtttcccct gcgctttcga ccagcaccga
    10921 accggtatca acgcgcgaga cggagtcttc aatgagggct ttgatctctt ttgccgcctg
    10981 ggcgctgcga ctggcaagat tacgcacttc gcccgccacc acggcaaaac cacggccttg
    11041 ttcacccgct cgcgcggctt caacagcggc attcagcgcg aggatattag tctggaaggc
    11101 aataccatcg ataacgctga taatgtcggc aattttcttc gaactatcgg cgatctcatg
    11161 catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact
    11221 ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggcgag
    11281 ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag tacgggagga
    11341 aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc
    11401 gcgcacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag
    11461 gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac caccggcaat
    11521 ttcgcgaata tgcgcaataa tttttgccag cggtgtaagc aacatacggc gaatgccgta
    11581 ccacgccgcc agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc
    11641 aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag
    11701 ggcgtactga gcaaacgctt ctcccattgc attttgcatt ccctgggttg gctgagcgaa
    11761 ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg ctgtgtgata
    11821 gtttttatat ttttcatcaa tattacgact ggtagcgacc atttcaggta acggtgccat
    11881 gcttttgaat tttttataat gagtcgcggc ctgggccaat gttttcctgg cgctatcgag
    11941 caattcaact ttggcattac tttgctgatt ggatgaatcc atcatcatcc gcaccgctga
    12001 acgactcagg ttaatgcgcg tttgtagcat taaatcccat gttgacgtca gctcgctctg
    12061 ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa gggaagaaaa
    12121 aaacagactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag
    12181 cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt
    12241 atcggcaagg aggggggaaa ctttattgct gatgccaccc gccgcgaaat tgaaataaaa
    12301 aacccgatgc gcagatcatc gggttcattt caattgagga catcgggaga attacgccac
    12361 ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    12421 caccagaatc aacatgcggt cgcccagtgc gcccagtcct gtgagatatt cggttgaaag
    12481 cgtcacggca aattccggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    12541 gacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
    12601 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    12661 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    12721 cacctgatcg tagccacgga tctcctgcac tttcagaata tcaataccgt actcttcatc
    12781 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    12841 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    12901 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    12961 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    13021 accacaacct ggtgttgacc aattaattga tccactagca aggcgtagcg gcgaccgcca
    13081 ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc gacgttgaac
    13141 actttccaca gttcgacgat aggcagatat tcaccccgca cttccagcac ccgctcgccg
    13201 ccagccagtg gatgcagatc ggcttcacgg ggttgcagtg attccatgac agcattcagc
    13261 ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcaagaat tgccagcgtc
    13321 agcggcagta aaatgcggat cgtagtgcca gtaccctgca tcgactggat ctcgacatga
    13381 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccacg cccggagacg
    13441 tcggtgacct gctccgccgt ggagaagcca ggtgcaaata tcagcattgc aacttcgtcg
    13501 tcgctcatgt tttcgctgac ggtcagacct tgcgaggccg cttttgccag aattcgttca
    13561 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    13621 gcagacagaa ttaaatttcc gacgctgttt ttacctgcag cgagccgttt ttctggcagt
    13681 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttca
    13741 atcaggctct tgtcgagttc agtagaactg cccaccagcg tcagttctac ctgcttgccg
    13801 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    13861 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    13921 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    13981 atggactggg tgatgaccag ctcaccgacg aggttaatta attgatcaac cttttctacc
    14041 gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc cacgcgaccg
    14101 gttggcgctt gttcggctgc cagtttaagc attggtgagg tggatatttt tggcgagacg
    14161 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    14221 tcatcttcgg cgatgtcgcc cggtaatatt gccgagaggg aatcagcccc tttcaccacg
    14281 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gccggccttc
    14341 aggcgcgaaa ggataattcg tcgcggtgac tgactacgac tctgctcatc ttgtggttca
    14401 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
    14461 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    14521 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    14581 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    14641 tgcgtggttt cctgcaaaac gctgaagcca aaggttcctg cccctccttt gatcgagtgg
    14701 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    14761 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    14821 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
    14881 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    14941 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    15001 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgacgac
    15061 acgtaacact ttgccgccat tcaacccgcc aaccatcagt tcgcggcggg atgcattggc
    15121 acgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt agggaaaatc
    15181 atcggtatga cccgagaggc taattcggtt gggaataccg ttcagtacgg gcgcgatggc
    15241 gcgcagaatg tcgcgcatat agggttcgac gtcggcactg ccagttctaa acatcgggcg
    15301 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    15361 tggacgtaac gcccgcagtt tcggatcgga ctcaatcaac tggtcgagat cgccacgcaa
    15421 tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg gccgcttatt
    15481 cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc tttcactatt
    15541 agaaatgcga tcgccgcccg taaccgctgt cgccagcgga gtccggaagt actccgcaat
    15601 ctgaatcagc tcttttggac tggagataga gatcagccac atcaccagaa aaaaggccat
    15661 cattgcggtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    15721 tttggcttta cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    15781 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ctcaataaac
    15841 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    15901 taaccgttca gatttgaaag cagagtgact ttgacgcact gcatcatttt gctggtttcg
    15961 gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaata
    16021 ccgaggaaag tccccaccat cgcatgagcg ataagcgccc ccagctcggc ggcaggacga
    16081 tcggctgaac ctaacgcgtg aactaccccc attacagccg caacaatacc aaaagccgga
    16141 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    16201 tcaatttctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    16261 aggcgcagat aatcgacgat aaattcaagc atgacgctat ccgcaaggat gcgtgggtag
    16321 ctggcgaaga tctcgctctc acggggattt tcaatatcgc gttccagcga aaacatcccc
    16381 atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat atacattgct
    16441 ttggtgtatt tggagcgacg aaacagcaac ggcagcgcct tcagcgtgcc tttaatcgct
    16501 ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat caccagttca
    16561 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    16621 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    16681 tgacaggatg ttcagtcgtc aggcgttaac acgcgatagg ggcaaaaaaa agcagcggta
    16741 cgtcgttacc gctgctggaa tgtttcgcca caccgtatca gttaaacagc ctgtactctc
    16801 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    16861 actgcccggg atggcggctg acataagctg caggcaaagc tgccaacagg ctggtgagca
    16921 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    16981 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gcggtccttc ttctgcttgc
    17041 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaag
    17101 ccagttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac attttgttcc
    17161 caggtcataa accagtcggt tgaaaatggc agcatgcctt tcggcggtgg gcttccgcgc
    17221 agttctttat agagttttat cagacgtccg cgacttaact gtgtttcgct ttccagcatc
    17281 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    17341 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    17401 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    17461 caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    17521 aaaccagttg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaacg
    17581 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    17641 tcagacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    17701 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtctcaccgc
    17761 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    17821 aagctgcaat aagcagaccc acctttttgg tttaataagt ccttacaaat agaagggggt
    17881 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    17941 atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatc
//
LOCUS       DAPQZD010000094        17984 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPQZD010000094 DAPQZD010000000
VERSION     DAPQZD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.094"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            33..1133
                     /gene="chaA"
                     /locus_tag="RO454_003077"
     CDS             33..1133
                     /gene="chaA"
                     /locus_tag="RO454_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDY0934874.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1538..1645
                     /locus_tag="RO454_003078"
     CDS             1538..1645
                     /locus_tag="RO454_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDY0934875.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(1794..2648)
                     /gene="kdsA"
                     /locus_tag="RO454_003079"
     CDS             complement(1794..2648)
                     /gene="kdsA"
                     /locus_tag="RO454_003079"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDY0934876.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2684..3493)
                     /locus_tag="RO454_003080"
     CDS             complement(2684..3493)
                     /locus_tag="RO454_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDY0934877.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(3497..3889)
                     /locus_tag="RO454_003081"
     CDS             complement(3497..3889)
                     /locus_tag="RO454_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDY0934878.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(3886..4719)
                     /gene="prmC"
                     /locus_tag="RO454_003082"
     CDS             complement(3886..4719)
                     /gene="prmC"
                     /locus_tag="RO454_003082"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDY0934879.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(4719..5801)
                     /gene="prfA"
                     /locus_tag="RO454_003083"
     CDS             complement(4719..5801)
                     /gene="prfA"
                     /locus_tag="RO454_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDY0934880.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(5843..7099)
                     /gene="hemA"
                     /locus_tag="RO454_003084"
     CDS             complement(5843..7099)
                     /gene="hemA"
                     /locus_tag="RO454_003084"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDY0934881.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7313..7936
                     /gene="lolB"
                     /locus_tag="RO454_003085"
     CDS             7313..7936
                     /gene="lolB"
                     /locus_tag="RO454_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDY0934882.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7936..8787
                     /gene="ispE"
                     /locus_tag="RO454_003086"
     CDS             7936..8787
                     /gene="ispE"
                     /locus_tag="RO454_003086"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDY0934883.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            8938..9885
                     /gene="prs"
                     /locus_tag="RO454_003087"
     CDS             8938..9885
                     /gene="prs"
                     /locus_tag="RO454_003087"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDY0934884.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            10010..11689
                     /gene="dauA"
                     /locus_tag="RO454_003088"
     CDS             10010..11689
                     /gene="dauA"
                     /locus_tag="RO454_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDY0934885.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(11744..12022)
                     /gene="ychH"
                     /locus_tag="RO454_003089"
     CDS             complement(11744..12022)
                     /gene="ychH"
                     /locus_tag="RO454_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDY0934886.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            12300..12884
                     /gene="pth"
                     /locus_tag="RO454_003090"
     CDS             12300..12884
                     /gene="pth"
                     /locus_tag="RO454_003090"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDY0934887.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            13001..14092
                     /gene="ychF"
                     /locus_tag="RO454_003091"
     CDS             13001..14092
                     /gene="ychF"
                     /locus_tag="RO454_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDY0934888.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            14307..15482
                     /locus_tag="RO454_003092"
     CDS             14307..15482
                     /locus_tag="RO454_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDY0934889.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            15593..15877
                     /locus_tag="RO454_003093"
     CDS             15593..15877
                     /locus_tag="RO454_003093"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0934890.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            15874..16728
                     /locus_tag="RO454_003094"
     CDS             15874..16728
                     /locus_tag="RO454_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0934891.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(17351..17707)
                     /locus_tag="RO454_003095"
                     /pseudo
     CDS             complement(17351..17707)
                     /locus_tag="RO454_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         4400 a         4579 c         4547 g         4458 t
ORIGIN      
        1 tcgataaatt catctgcaaa gggataacca taatgtcaaa tgctcaagag gcggtaaaaa
       61 cccgccacaa ggagacttcg cttattttcc cggttctggc gctggtagtg ctgttcctgt
      121 ggggaagcag ccagacacta ccagtggtca ttgccatcaa tcttcttgcg cttattggta
      181 ttttaagtag cgcctttagt gttgtccgtc atgcggacgt attagcccat cgcctgggag
      241 aaccttacgg ttcgcttatt cttagccttt cagtggttat tcttgaagtc agtttgattt
      301 cagctttaat ggcaaccggc gatgccgcgc caacgctaat gcgtgatacg ctctattcaa
      361 tcattatgat tgttacaggt gggctggttg gcttttcatt attgttgggc ggacgtaagt
      421 ttgccaccca atatatgaat ctgtttggta tcaagcagta tttaattgcg ctgttccccc
      481 tggcgataat cgtactggta tttccaatgg ctctgcctgc ggcgaatttt tcaacaggtc
      541 aggcgttact ggtagcatta atttctgcgg caatgtatgg cgtatttttg ctgatccaga
      601 ccaaaacgca tcaaagtttg tttgtctacg agcacgaaga tgacagtgat gatgacgacc
      661 cgcaccacgg taaaccgtct gcccatagca gcctgtggca tgccatctgg ttgattgtcc
      721 atctgattgc cgttattgca gtcaccaaaa tgaacgccag cccgctggag acattgctcg
      781 acagcatgaa tgcgcctgtc gcctttactg gcttcctggt ggctctgttg attctgtcgc
      841 cggaaggttt aggtgcgtta aaagcagtgt tgaataacca ggtccagcgc gcgatgaatc
      901 tgttctttgg ttcagtgttg gcaaccattt cgcttaccgt acctgtcgtc acgctaattg
      961 cctttatgac gggtaacgaa ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg
     1021 tggcctcttt agtactgtgc catatctcct tctccaccgg acgaactaac gtgctcaatg
     1081 gcgcagcgca tctggcactg tttgccgcct atttgatgac gatatttgcc tgacagttat
     1141 atctcctccg atgacggaaa tcgggggaga taatataaat tcctgcttat tatatttctt
     1201 catactagct ggacaggttg ttaatcagaa ttgcaagcct gtaccacaac ctgcacgaaa
     1261 tgactgcaac agctgttgaa aaaccggaat gtactcttta ttgcaaacat ttcagggatg
     1321 aagtgaagat gaattgcgac gtagtatcca caccaagccg gcgttatccg gtgaggcgca
     1381 atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa cccccgcacg
     1441 ttgagaggta caacctgaca acagaacggg ggctaatctt gaaccagaac aattcaagaa
     1501 tagccgcaaa atgttgtcat tacaacaagg cggctatatg acgctcgcgc agtttgccat
     1561 gattttctgg cacaacctgg cggcaccgat cctggcgggg attattaccg cagtgattgt
     1621 cagctggtgg cgtaaccgga agtaaggtga aaacgggcgt caggctgccg tgatggcaat
     1681 atgcgcccga aaccggccgc aggggtaaca gcctgcggcc ttttttgtat ccggcaccca
     1741 tgaaaaaagt cttaacgcag aacgctaaga ctttattttt caggcaaaaa agattacttg
     1801 ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttcat ttgcttgagg
     1861 aacggttcca gtttagccag cggcagcgcg gatggaccat cacatttcgc atgttccgga
     1921 tccggatgcg cttcaataaa cagccccgcc agacctaccg ccataccggc tcttgccagc
     1981 tcagccacct gagcacggcg accgccggaa gctgcgccaa atggatcgcg acattgcagt
     2041 gcgtgggtaa cgtcgaaaat caccggcgaa ttaccagaca ctttcttcat gatgctgaag
     2101 cccagcatat cgacaaccag gttgtcatag ccgaagttag caccgcgatc gcaaagaatc
     2161 actttttcgt tgccgccttc tttgaattta tcaacgatat tacccatctg tcccgggctg
     2221 acaaactgtg gtttcttgac gttaattacc gcaccggttt tcgccatggc ttcaaccagg
     2281 tcagtctggc gagcaagaaa cgccggcaac tgaatcacat ccacgacatc agcaacgggc
     2341 tgtgcctgac ttggttcgtg aacgtcggtg ataattttca cgccaaaagt ctgcttcaac
     2401 tcctggaaga ttttcatccc ttcttccagg cccggtccac gataagagtg gatggaggag
     2461 cggttggctt tgtcaaaaga ggctttgaac acgtaaggga tacccagttt ctgggtcacg
     2521 gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag attccagcac gttcataccg
     2581 ccaaacagta cgaacggcag gtcatttgct acgttgatgt caccaatgct aaccactttt
     2641 tgtttcatag gatcgcctta atcttgagta aaatgtcgat taattaatgc agcacaatat
     2701 gtttatgcgc gatgttattt atttgcgcac ggatcatttc gctgatcggg tcttccggac
     2761 actgttcaac gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc agttgcgcat
     2821 aaatcaaccc gcgatcgcga atttcatagg gatcttccgg gttgaattgt aataaagctt
     2881 cgctggtgcg taacgccagc tccatctgat tttcttccat caacgaagct ttgagtgtat
     2941 ccagcaattt gcggattacc tcaatgttat cagcttcatc aaggtcttca taaaacagtt
     3001 ccgccgacgg gctgatattt ccctttaacc acacatccag catatgttcg cttaacgatt
     3061 caccgttaaa aggattaatc agccaaattt cgccatccgg acattcaatg cgcaatatca
     3121 gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg acccataata
     3181 aaaccgcacc taatgatacc gcactgccct gtcgattctt taacacctgg tccagccata
     3241 atgcatcgga aagacgataa acgccgcgtg aggctttaaa tccccagtcg ccgtaaaaca
     3301 gcgcgatcag tttttccaac tgctcttcta aaggcagaag ctggctaatt tcttccttcg
     3361 ccagactaac gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa tcgcggcgga
     3421 ttgcttcgca agccaggatc atgccttcgc acaatggcgc tttattaaat tcgaaatcag
     3481 ctaacgatct catgacttac cccagtaacg gtacttttgt ggtggcgagt ttaatgatga
     3541 tgtacagcac caccaacgcc agcgggaagg caataatgcg cgcctgctga ctgtgcatcc
     3601 gacgataatc gagtgcaata aaacccaaaa cgatataaat gataactcca aacagctttt
     3661 cagtcagcca ctgtgcctgc ccggaaaatg gctggatgtg cgctttagcc atcaacgcaa
     3721 tgccgcttaa caataacagc gtatcgacaa ccggcggcac aatgcgcgtc cagcgagcaa
     3781 atgcctgtgg atgcttctga taacgtagcc agaaacgtaa ggtcaatagc ccaacagaaa
     3841 gcgcgatact aatgagatga acgctaagca acgtagaaaa acttgtcatt gataatagcg
     3901 gccgagcgtt acgcgctcgt tatccccata gtcacggcag gtttcgacgt catgataccc
     3961 cgcgaggata aatgcctgtc gcaccgcttc accctgctgc cagccatgct ccagaagcag
     4021 aaagccgccg gataccagcg cgttacgcga ctgttcgatg atatgcacga tgtctgccat
     4081 tccactgtct gccgcaacca gcgcagtgag cggctcaaag cggacatcgc cttgttgaag
     4141 atgtgggtcc tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa actgctgccc
     4201 ggctagcgcg ctaaaccagt cgctttgcag aatgtggata tttttgatag ccagatgctg
     4261 ggcattacgt tgtgccaggg cgacagcatc gggcatacga tctacagcga taatttcgca
     4321 gtccgggcgc tcgctggcaa gcgcaagcgc aatcgccccg gtacccgttc cgagatcgag
     4381 aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac actccgtatc
     4441 cgggcgcgga attaaggtcg ctggcgaaac aaataacggc aacgaccaga attctcgcac
     4501 cccggttaaa tgagcaatgg gttcaccatc gcgacgacgc gtcagtagcg catcaagttg
     4561 ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg agaataaaag tacgcccttt
     4621 gccggtaaca tgctccagca ggatttcagc atcacgccgc gggctttcgc tcgcctgaag
     4681 ttggcttatt gcttcacgta accagtgttg atattccatt attcctgctc ggacaacgcc
     4741 gccagttggt cggcctgatg ttcctggata atcggttcaa tcagcatatc cagcttacct
     4801 tccatcactt catccaggcg gtagagcgtc aggttgatgc ggtgatcggt aacgcgcccc
     4861 tgcgggaagt tgtaagtacg gttacggtcg ctgcgatcgc cactccccag caggttacga
     4921 cgggtagacg cttcggcctg ttgccgtttt gccatttcag cagcgtggat gcgagcaccg
     4981 agtacagaaa gtgctttagc tttgttttta tgttgtgaac gttcgtcctg acattcaaca
     5041 acaatcccgg tcggcaagtg agtaatacga attgccgaat cggtggtgtt aacgtgctga
     5101 ccacccgccc ctgacgagcg gaaagtatct atgcgcaaat ctgctgggtt gatgtccggc
     5161 agttctgcat caggcagttc tggcattacc gcaacggtac aagcagaagt atgaatacga
     5221 ccctgcgatt ccgtagcagg aacacgctgc acgcgatgac cgccagattc gaatttcaaa
     5281 cgaccatata caccatcacc gctaattttg gcgatgatct ctttataacc accatgttca
     5341 ccctcgctgg cgctcatgat ttctacacgc cagcggcggg cttcggcgta acggctgtac
     5401 atacggaaca gatcgccagc gaacagcgcc gcttcgtcgc cgccggttcc ggcacggact
     5461 tcgaggaagg cgttacgttc gtcatcagga tcttttggta gtaacagaac ctgtaattgc
     5521 tgttccagtt gctcgctttt ttctttagct tcgcgcagtt catcctgcgc catctcacgc
     5581 atttcaggat cgtcaagcat catctgtgcg gtttcgatat cttcctgaac ctgttgccag
     5641 tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa tgcgcgaaaa
     5701 cgttcctggt cggcgatagt ttgcgcgtca cccagcaacg cctgaacttc ttcatggcgt
     5761 tcatgcaggg cttccagttt ggcaacgata gaaggcttca taggcgtaaa tgcaccctgt
     5821 aaaaaaagaa aatgatgtac tgctactcca gcccgaggct gtcgcgcaga atattcaggc
     5881 gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt tgttggcgca tggatcaagc
     5941 ggttagtcag tttccatgcc agatcctgca taatggcttg tgcgtcgccg ccctgctcaa
     6001 gggccgctaa cgctttggcg gttaactcat cgcgaacgtg ctctgcctgg ctgcgatact
     6061 cgcgaatagt ttcgctggcg ctttgtgcac gcagccacgc cataaattcg ctggtttcct
     6121 gagcgacaat agtttccgcc tcaaccgctg cggctttacg ctgcgccagg ttgtgcgaaa
     6181 tgatgctttg cagatcgtcc acgctataaa gataagcatt cgccagtttg ccaacttccg
     6241 gctcaacatc gcgcggaaca gcaatatcca ccaacagcat tggttggttg cgacggcttt
     6301 ttaatgcgcg ctccaccatg cctttcccga taatcggtaa cgggctggcg gtggaactga
     6361 tgatgatatc ggcttcgcgc agacgttcgt cgatatcact cagggcaata atttccgcgc
     6421 cgacttcatc tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata atcatcttct
     6481 gtactttgtg ttcgcgcaga tgacgcgcca ccagctcgat ggtttcgccc gcacccacca
     6541 gcagtactgt caccgtagag agcgattcaa agatctgccg cgccagcgta caagccgcaa
     6601 aagcgacaga cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt ttcgccacag
     6661 agaaagattt ctgaaacatg cgttctagtt cgctggcctt catatgacct ttttgcgaat
     6721 cggcaaacgc ttttttaacc tgaccgagga tctgcggctc gccaaggacg agagaatcaa
     6781 gcccactggc aacgcgcatt aaatgactga ccgcatcgtt atcctgatgc cagtagaggc
     6841 tattacgcag atcgtcttcg ttgagattat ggtaatcaca caaccagcga atgagcgcct
     6901 cttgcaggtc atcccgttct tcaacgctga gataaagttc tgtgcggtta cacgtcgaca
     6961 gcaccacgcc gccctgcacc atcggctgcg cgagcagact gtcaagcgcc tgatcgagct
     7021 tatccggcga aaacgataca cgttctcgca gcgatacagg tgccgtttta tggttgatac
     7081 cgagtgctaa aagggtcatg tctgcgggaa ataataccaa cgttgatagg gttagtctgc
     7141 ttgcatcata caggatgcgt aagatcaata aaagagagcg cccccttttg gagtaattgc
     7201 cgaaagccgt tagattctgg caattaagac aacgtgaaca tagacgatgg ctaacggtaa
     7261 cgctagcatt aagggttata actgcaacgt atctcaagga cttgtcatta ttatgaccct
     7321 gcccgatttt cgctttatcc gcctgctacc gctagctgcc ctcgtgctca ctgcctgttc
     7381 tgttaccaca cccaaaggcc caggcaaaag cccggattcg ccacagtggc gtcagcacca
     7441 gcaagacgtg cgtaatctta atcagtatca gactcgcggt gcattcgctt atatttccga
     7501 ccaacaaaaa gtgtacgctc gcttcttctg gcaacaaacc ggccaggatc gctatcgtct
     7561 gctgttaact aacccattgg gcagcacgga actggagctg aatgctcagc cgggtaacgt
     7621 acaattagtc gacaataaag gtcagcgtta caccgccgat gacgccgaag agatgattgg
     7681 taaactaacc ggaatgccga ttccgctcaa cagcttgcgc cagtggattt taggtttacc
     7741 gggtgatgcg accgattaca aactggacga ccagtaccgc ctgagcgaaa tcacctacag
     7801 ccagaatggc aaaaactgga aagttgttta cggtggttat gacacaaaaa ctcaacctgc
     7861 gatgccagcc aatatggaac tcaccgacgg tggtcaacgc atcaagttaa aaatggataa
     7921 ctggatagtg aaataatgcg gacacagtgg ccctctccgg caaaacttaa tctgttttta
     7981 tacattaccg gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt tcagtttctt
     8041 gattacggcg acatcatcag cattgagctt cgtgacgatg gggatattcg tctgttaacg
     8101 cccgttgaag gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg gttgttgatg
     8161 aaaactgcgg cagacagcgg gcgtctttcg acgggaagcg gtgcgaatat cagcattgac
     8221 aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc gacggttctg
     8281 gtggcgttaa atcatctctg gcaatgcgga ctaagcatgg atgagctggc ggaaatgggg
     8341 ctgacgctgg gcgcagatgt tcctgtcttt gttcgtggtc atgccgcgtt tgccgaaggc
     8401 gttggtgaaa tactaacgcc ggtggatccg ccagagaagt ggtatctggt ggcgcaccct
     8461 ggtgtaagta ttccgacacc ggtgattttt aaagatcctg aactcccgcg caatacgcca
     8521 aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca atgattgcga ggttatcgca
     8581 agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata cgccccgtcg
     8641 cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg atacagagtc tgaagcccgc
     8701 caggtgctag agcaagcccc ggaatggctc aatggctttg tggcgaaagg cgttaatctt
     8761 tccccattgc acagagccat gctttaagcc gggcaagctg agtttcggtg acaacgtcac
     8821 cttgttccag acgttgcatc gcgctcttta atacaccgcc tggaaagaat catgcctggc
     8881 ccgcacagtt ttcggcagat tctttccacc aatggacgca tgcctgaggt tcttctcgtg
     8941 cctgatatga agctttttgc tggtaacgcc accccggaac tagcacaacg tattgccaac
     9001 cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg cgaagtcagt
     9061 gtacaaatta atgaaaatgt acgcggtggt gatattttca tcatccagtc cacttgtgcc
     9121 cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg tcgtgcttcc
     9181 gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga ccgtcgcgtc
     9241 cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc cagcgtcggt
     9301 gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt cttcgacgtt
     9361 ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca gctgaatctg
     9421 gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg cgctatcgct
     9481 aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg tgcgaacgtt
     9541 tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact ggtcgatgat
     9601 atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag ctctgaaaga acgtggtgct
     9661 aaacgtgtat ttgcgtacgc gactcacccg atcttctctg gcaacgcggc gaacaacctg
     9721 cgtaactctg taattgatga agtcgttgtc tgcgatacca ttccgctgag cgatgaaatc
     9781 aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta tgctggccga agcgattcgt
     9841 cgtatcagca acgaagaatc gatatctgcc atgttcgaac actaatcgaa cccggctcaa
     9901 agacccgctg cggcgggttt ttttgtctgt aatatccatt tgtatgacct atgcctcctt
     9961 cacctgccat ttagttgaca gatgatgcgc tcacggatga aacattattg tgaacaaaat
    10021 attttcctca catgtgatgc ctttccgcgc tctgatcgac gcttgctgga aagaaaaata
    10081 tactgccgca cggtttaccc gtgacctgat tgccgggata accgtcggga ttattgctat
    10141 cccgctggcg atggcgttgg ctattggtag tggtgtggca ccccagtacg gtttatatac
    10201 cgcagctgtt gcggggattg tcattgctct gacgggaggg tcacgcttta gcgtttccgg
    10261 tccgactgcg gcatttgtgg taattctcta tcccgtttcg caacagtttg gactggcagg
    10321 actgctggtt gcgaccttgc tgtcggggat ctttttgatt ctgatggggc tggcacgctt
    10381 tggtcgcctg attgagtata ttccggtttc cgtcacctta ggtttcacct cgggtatcgg
    10441 gatcaccatc ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg cccatgtccc
    10501 ggaacattat ctacaaaaag tcggcgcatt atttatggcg ctgccgacca ttaatgtggg
    10561 tgatgcagcc attggcattg tgacgcttgg cattctggtt ttctggccgc gtctgggcat
    10621 acgtttaccc ggccaccttc cggccttgct ggctggttgc gcggtgatgg ggattgttaa
    10681 cctgctcggc ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc tggccgatgg
    10741 ttctcagggt aacggtattc cgcaactgct gccgcaactg gtgctgccgt gggatctgcc
    10801 taattcagaa ttcacgctaa cctgggattc tattcgcaca ctgctgcctg cggcattctc
    10861 aatggcaatg ctcggcgcaa tcgaatctct gctctgcgcc gtggtgctgg atggtatgac
    10921 cgggacgaaa cacaaggcga acagcgaact ggttggacag ggactgggga atattatcgc
    10981 tccgttcttt ggtggcatta ccgctacagc tgccatcgcg cgttctgccg ctaacgtccg
    11041 tgccggggca acttccccta tctcggcggt gatccactct attctggtta ttcttgccct
    11101 gctggtactg gcaccgctgc tctcctggct gccactttcc gccatggcag ccctgctgtt
    11161 gatggtggcg tggaacatga gtgaagcgca caaagtggtc gacttgctgc gtcatgcacc
    11221 gaaagatgac atcatcgtca tgctgctgtg catgtcgctg accgtgctgt tcgatatggt
    11281 tattgccatc agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc gtatcgcacg
    11341 tatgactcgc ctggcaccgg tagtcgtaga cgttccagac gatgtcctgg ttctgcgcgt
    11401 tattggcccg ctgttttttg ctgccgctga aggcttattc acggacctgg agtcacgtct
    11461 tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt ccggtacttg atgctggtgg
    11521 tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac tgcgcgtgtg
    11581 caacctggaa ttccagccac tgcgcactat ggctcgcgca ggcattcaac cgatcccggg
    11641 acgcctcgca ttcttcccga atcgtcgcgc ggcgatggcg gatttataag tacgagattg
    11701 accagtcagc gcaaactgac tggtcagcaa actgcattat ttgttagcta tgacggcgat
    11761 tatcgctacg gcggcaacgt ttgtcatagt ggcgaaccca ccagtaacgg tcgcacacct
    11821 gttcatgccc gccaacacgg gcacccgcca gccagagaat cgcaccgacg aaaatactta
    11881 gcacagcacc atgtgcgaaa tactggggca tattaaactg tggtaactgg ttgaggattg
    11941 aataccccac gccgaccacc attaccacca gacccaaccc catgagcacg ttaccgagta
    12001 acgaagcgtt tttgcgtttc atatgtcacc tccggaactt tctgggttgt aacagggaat
    12061 acccctcttc cttatgtgta aagtatagac aacaccctgt gggttatgtg cgggtgtgat
    12121 cacaattaca acccttattt caacaaaact ttacaaataa acgcctgaac accttgactt
    12181 tctaatagtc cacttaggca ataattgcat tatctgcatt tgatacgcag ttttttgtca
    12241 agcggggccg caaccagtaa actacgcgcc agttatgtac acactcagga caaaaaaacg
    12301 tgacgattaa attgattgtc ggcctggcga accccggtgc tgaatacgcc gcaacgcgac
    12361 ataatgctgg tgcctggttc gttgacttac tggcagagcg attgcgcgct ccgctgcgcg
    12421 aagaggctaa attctttggt tatacttcgc gagtcaccct tggaggcgaa gatgtccgcc
    12481 tgttagtccc gactacattt atgaatctca gcggcaaagc cgttgcggcg atggccagtt
    12541 ttttccgcat taatccggac gaaattctgg tggcccacga cgaactggat ctgcctcctg
    12601 gcgtcgccaa atttaagttg ggtagcggtc atggtggtca caatggactg aaagacatca
    12661 tcagtaaatt gggtaataac cctaactttc accgtttacg catcggaatc ggtcatccgg
    12721 gcgataaaaa taaagttgtc ggttttgtgt taggcaaacc gcctgttagt gaacagaagt
    12781 taattgatga agccatcgac gaagcggcgc gttgtactga aatgtggttt acagatggct
    12841 tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt gtctgcggca
    12901 tttttgccga gtgccgtgta taataggcaa agttatttcc atttctgcaa tctgttagca
    12961 ataacaggtt gattattaag atattaaggt gatttaaatc atgggattca aatgcggtat
    13021 cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac gcgctgacca aagccggtat
    13081 tgaagcggcc aactttccat tctgcaccat tgagccgaac actggcgtcg taccaatgcc
    13141 tgaccctcgc ctggatcaac tggctgaaat cgtaaaaccg cagcgtacac ttcccacgac
    13201 catggaattt gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg aaggtctggg
    13261 taaccagttc ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg ttcgctgctt
    13321 tgaaaatgac aacatcattc acgtttccgg caaagttaac ccggcggatg atattgaagt
    13381 tatcaacacc gaactggcgc tggcggatct cgacacctgt gaacgtgcga ttcatcgcgt
    13441 acagaagaaa gccaaaggtg gcgataaaga cgcgaaagcc gagctggcgg tactggaaaa
    13501 atgcctgccc cagctggaaa acgcaggtat gctgcgcgcg ctggatttaa gcgctgaaga
    13561 gaaagcggct gttcgttacc tgagcttcct gacgctaaaa ccaacaatgt acatcgccaa
    13621 cgtcaacgaa gacggttttg aaaacaaccc gtatcttgac caggtgcgtg aaatcgcagc
    13681 gaaagaaggt tctgttgtag ttccggtttg tgctgctgtt gaagcagaca ttgccgaact
    13741 ggacgacgaa gagcgtgacg agtttatgca ggagcttggg ctggaagagc cgggcctgaa
    13801 ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa acttacttca ccgctggggt
    13861 gaaagaagtg cgtgcatgga ccattccggt tggtgcaacc gcgccgcagg cagcgggcaa
    13921 aatccatact gattttgaaa aaggctttat ccgtgcacaa accatctcgt ttgaagattt
    13981 catcacttac aaaggtgaac aaggcgcgaa agaagcaggc aaaatgcgtg cagaaggcaa
    14041 agattacatc gttaaagatg gcgatgtgat gaacttcctg ttcaacgtct aaattaaatc
    14101 tgctgtctca tgaggtttca ctgaatctca taagaaccgt aaaactctat aaaatccacg
    14161 caattgcgtg gatttttcat ttcgcaatgt ctcaattcat ctcataacaa cgctgtcaaa
    14221 aatgagtaca tggatgagta caacattctt ccatcttcat tttaagtgag tacaataacg
    14281 gtataataaa taaacaaggc cccgttatgc tcactgatac gcaatgccgc actgctaagc
    14341 cgaaagaaaa actctatcga cttaatgatt tcaatggtct ctacctcgaa gtgaaaccca
    14401 atggtaaaaa ggcatggcgc tatcggttta aactcaacgg taaatccagc atgtttgcgc
    14461 tgggtgagta cccgacggta aaacttgccg aagctcgcga gaaatgcgag caagcgcgta
    14521 agcaagtcgc agatggagtt agcccaacac aggctcgtca gttagataag atccgcaagg
    14581 tcaacgacgc atccaacacc tttgagctaa tcgctaaaga atggttgcag atgaaagact
    14641 gggccggaat taccaaaacg cggcgactgg atatgcttga acgtgtggtt tttcccgcca
    14701 tcggtaagct ccctgtcaga gagatcactc cacaccatat acttaaaatt cttcaggaaa
    14761 ccgctaaacg aggagcacca acagttgccg ctgaagcccg tcgcaccatt tcatcagttt
    14821 ttgagttggc agtcgcgacg cttagagctg atagcgatcc cgtatggcct gtgcgcaagg
    14881 cccttccggc taataagaca cagcacaaac aggcactaaa tccccaacaa atcggaaagt
    14941 tactaagctg ttttgacaat agtcgtggct cgtatcaggt taactattgc atgtggctta
    15001 tgtggtggac attggcgcgt cctgcagaag ccaccgaagc agaatggaca gaattcaatc
    15061 ttgataacgc cctatggact attccggcag cgcgaatgaa agcccgcaga gaacacgtta
    15121 ttcccctacc cttccaagct gtcaaaatgc tcagaacact acagggatta accgggcatc
    15181 ggcagcacct tttcccgggc agagacaatc cgctaggtcc aatgacatcg cactcactgc
    15241 gccagctact taaaagcctc ggctggagtg gtacatatag cccacatgca acccggacca
    15301 caggaagtac ccgtttaaac gagttgggct atcgacctga tgctattgag gcccaacttg
    15361 cgcacgctga taccaacaat gttcgtcgca catacaacca tgcaacttat cttgatgagc
    15421 gtaaagtgat gatgcaggaa tgggctgata tgttggacaa atgggtagca ggattcaagt
    15481 agaacttgct gcacaaagtg aaccacgagg atagaactta ttcctcaagg ttctggagtg
    15541 cccccaagcc cgtagacaaa tctgccctat agtttgagca taggaggagt ctatgggcac
    15601 accacgattt accccagagt ttaaggaaga agctgtccgt caggtcactg aacggggcta
    15661 ttccgttgcc gatgtttctg aacgcctcgg cgtatcggct cacagccttt ataagtgggt
    15721 ccggtcagtc agaccggata acagcgggca tcaggcgcag gatttactgg atgcgcggac
    15781 ggagattctc aggcttaaag cgcagcttaa acgtactgag gaagaacggg atattctgaa
    15841 aaaggcagcg cggtactttg caagggagcc cgactgaagt accgcttcat caacgatcac
    15901 cgtgaaatct ggtcgatcgt aacaatgtgt cgggttctga aggtcgcccg tgcgggattt
    15961 tacgtctggc ttcatcgccc tgtttctgcc ggagaaaagg ataatcagcg gttgctggag
    16021 ctcatccgcg atgcttatgc actgagcggc ggagtctacg gttatcgcag ggttcacggc
    16081 gatcttcgtg aaatcggtga agtatgcagc agaaaccgtg ttgccagaat catgaagcgg
    16141 aacaggatac aggccgtaca tggctataag gtcccgcgtg gcgtcagggg aaggccgtca
    16201 ctgatagctc ctaatcgcgt acagagggaa tttacgatgg taaaacccaa ccaggtgtgg
    16261 gtgactgata ttacctacat ccgcacctgg cagggttggc tgtatctggc ggtggttatc
    16321 gatctttttg cccgtaatgt ggtgggctgg tcgatgaaac caacgctgtc gcgcgagctg
    16381 gcactggatg cgctgctgat ggcagtctgg cggcgtaagc ctgcagaaaa cgtcatagtg
    16441 cacagcgatc agggcagtca gtatggcagt gatgactggc agcggttctg cagggccaac
    16501 aatctggcgc cgagtatgag ccgtcgtggc aactgctggg ataatgcggt ggcagaatcg
    16561 ttcttcagtt cactgaaaaa agagcgcatc agaaagcgga tatataaaac ccgggacatg
    16621 gcccgggcgg atatcttcga ttacattgaa gtgttctaca accggacccg gcgccacagt
    16681 catctgggtg gcgtcagccc ggaggccttc gaaaaggcct caccgtgagg acagaaactg
    16741 tctaccggaa tgtggtcact ccacttgagt cgtcttgctg tactcctttg gccaatccat
    16801 ttagctctcc aacgtgatgt tttcaacggt aacaaaagta acaatataac atgcagtcat
    16861 ttcaacgatc tcagtgttac cacccatttt gagcaatcat atcaaagtaa caaaagggga
    16921 atattgtgtg gttcatcgct gaaagcaggc taagatcacc tgccggctat gcatatcatg
    16981 aaagtgctaa atgtcccgtc ttgtttatca accgagataa gctgtaattg actgagtggc
    17041 tccccttaat gctttttctc aatcaacaga tatggatggg accagtcagt gacctcttta
    17101 tatgaaaaaa acccatcgtg ctccagtgcg caaacgccct tatgactggc aacgtaatcg
    17161 taaacagccc ggctttccag tccctgtaac atgtgcgaaa gcagctttcg gcgcatcaca
    17221 tctgacttcc tgcgtttact gtcaacaacg ggtaataaag tgatccacta accgtcacca
    17281 ccaacggttt aatattgatc caccttgttt actcaggatt agcttcaggt ataaccccgg
    17341 cctttcgttt ctacttcagt cgatagctct ctccttttat ctggactacg tgtgagtgat
    17401 gtaagatacg gtccagcatc gccgatgtca gggctgcatc cctggcgaac gtctgatccc
    17461 actgcccgaa cggcaggttg gagtccagga tcatcgcgct cctctcgtaa cgtttagcga
    17521 tgacctggaa gaacagcttg gcttcttcca gactgaacgg cagataacct atttcatcga
    17581 tgataagtag cttcggggcc atgacaccgc gattgagtgt cgttttgtaa cggccctggc
    17641 gctatgaagt ggatagctgt agcagcaggt ccgctgttgt gaagcgaacc ttgatgcctg
    17701 cccgtagcgg cggacgccca tggcacaggt gccgcaggcg agcatgcgtt caacggagag
    17761 tcgggtccat tagctgatgc tgtcagcgta tttttcgagg aatttatgta agaagcgtca
    17821 aaatgaaagc gccaactaat aatgacagaa ttaataaaat accagtaagc gcgtatgcta
    17881 taccttttcc aatctcgttt tttatggtac gtccaattga ataaaggatt aaagataccg
    17941 atagcaaaac tatcaaaata aaaccataca ctaacgctgc aagt
//
LOCUS       DAPQZD010000095        17715 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPQZD010000095 DAPQZD010000000
VERSION     DAPQZD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.095"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            253..1152
                     /gene="yegS"
                     /locus_tag="RO454_003096"
     CDS             253..1152
                     /gene="yegS"
                     /locus_tag="RO454_003096"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDY0934892.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1234..2007)
                     /locus_tag="RO454_003097"
     CDS             complement(1234..2007)
                     /locus_tag="RO454_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0934893.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2113..3153)
                     /gene="gatD"
                     /locus_tag="RO454_003098"
     CDS             complement(2113..3153)
                     /gene="gatD"
                     /locus_tag="RO454_003098"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDY0934894.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3201..4555)
                     /locus_tag="RO454_003099"
                     /pseudo
     CDS             complement(3201..4555)
                     /locus_tag="RO454_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(4559..4843)
                     /gene="gatB"
                     /locus_tag="RO454_003100"
     CDS             complement(4559..4843)
                     /gene="gatB"
                     /locus_tag="RO454_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDY0934895.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4874..5326)
                     /gene="gatA"
                     /locus_tag="RO454_003101"
     CDS             complement(4874..5326)
                     /gene="gatA"
                     /locus_tag="RO454_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDY0934896.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            complement(5336..6598)
                     /gene="gatZ"
                     /locus_tag="RO454_003102"
     CDS             complement(5336..6598)
                     /gene="gatZ"
                     /locus_tag="RO454_003102"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDY0934897.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6627..7481)
                     /gene="gatY"
                     /locus_tag="RO454_003103"
     CDS             complement(6627..7481)
                     /gene="gatY"
                     /locus_tag="RO454_003103"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDY0934898.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7708..8760)
                     /gene="fbaB"
                     /locus_tag="RO454_003104"
     CDS             complement(7708..8760)
                     /gene="fbaB"
                     /locus_tag="RO454_003104"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDY0934899.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEESSAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9017..10294
                     /locus_tag="RO454_003105"
     CDS             9017..10294
                     /locus_tag="RO454_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0934900.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10291..11295
                     /locus_tag="RO454_003106"
     CDS             10291..11295
                     /locus_tag="RO454_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDY0934901.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11292..12257
                     /locus_tag="RO454_003107"
     CDS             11292..12257
                     /locus_tag="RO454_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0934902.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12231..12977)
                     /locus_tag="RO454_003108"
     CDS             complement(12231..12977)
                     /locus_tag="RO454_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDY0934903.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13029..13847)
                     /locus_tag="RO454_003109"
     CDS             complement(13029..13847)
                     /locus_tag="RO454_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDY0934904.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(13912..14712)
                     /gene="thiD"
                     /locus_tag="RO454_003110"
     CDS             complement(13912..14712)
                     /gene="thiD"
                     /locus_tag="RO454_003110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDY0934905.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14709..15497)
                     /gene="thiM"
                     /locus_tag="RO454_003111"
     CDS             complement(14709..15497)
                     /gene="thiM"
                     /locus_tag="RO454_003111"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDY0934906.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15532..15628)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15720..15992)
                     /gene="rcnR"
                     /locus_tag="RO454_003112"
     CDS             complement(15720..15992)
                     /gene="rcnR"
                     /locus_tag="RO454_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDY0934907.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16113..16937
                     /gene="rcnA"
                     /locus_tag="RO454_003113"
     CDS             16113..16937
                     /gene="rcnA"
                     /locus_tag="RO454_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDY0934908.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17156..17494
                     /gene="rcnB"
                     /locus_tag="RO454_003114"
     CDS             17156..17494
                     /gene="rcnB"
                     /locus_tag="RO454_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDY0934909.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17576..>17715)
                     /locus_tag="RO454_003115"
     CDS             complement(17576..>17715)
                     /locus_tag="RO454_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0934910.1"
                     /translation="EIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKY
                     D"
BASE COUNT         4346 a         4588 c         4287 g         4494 t
ORIGIN      
        1 gactttaata tattttgaat agtatattga agatgccact gtttagttga atattaggta
       61 tattctcttt tttaaaattt atcagtggct accgttaata ttcgctgtcc ccattgtaag
      121 gtcctggtgg taagcactga atccccatac ctgaactgac tttttatcct ccgactttca
      181 tcctgttctg actccacctt ttcttttctg ctctacacta tctacagacc aatcataaag
      241 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact
      301 gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat
      361 gtgcggatca cctgggagaa aggcgatgct gcacgatttg tagaggaggc ccggaagtta
      421 ggcgtcgcaa cagtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggca
      481 ttgattcaat gtgaggggga tgacataccc gcgctgggaa ttttaccatt aggaactgcc
      541 aatgattttg ccactagtgt agggattcct gaggcactgg ataaggcgct gaaactggca
      601 attgccggta acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
      661 aatatggcga caggcgggtt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
      721 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga cactctgcaa
      781 ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
      841 ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa cgcattaatt
      901 aacgatggct tgctgcaact gcgcattttt accggcgatg aaattattcc gactctcgta
      961 tcaaccttaa aatctgacga agataacccg aatattatcg aaggtgcttc gtcgtggttt
     1021 gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagtgggcaa
     1081 aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca
     1141 ttgttgcgtt aatcaaacta ttctctgttg aaccagagag aatccgtaat gccggatacg
     1201 gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt
     1261 cttctgcaat gccagtttat ccacctcaga cacagcatca tcggtaataa taatgtcaat
     1321 tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg aatcaagaac
     1381 ggcaatcact ttattcgccg ctgtcaccat cgcaccgcta acggaataac cttcattaaa
     1441 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
     1501 aatatcctgc agggaacgtt cagcaataga tccatgcatc gagcgcgttt tatggcggac
     1561 cgtgccaccg cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgccgccgg
     1621 gagactgttg gtaatcaccg tgatattttt ggcggtcatt aatccttcgg cgataagcat
     1681 tgtggtactt ccgctgtcga gaataacggt catcccttcg tggatcattt tgactgccgc
     1741 ctgcgctatt ctgtttttcg gcgcgctggc gagctgaaat cgctctttaa acccgacttc
     1801 ctgggtctcg gtttcgctat tacctgacat tattttcgcc gcaccgccat gaaagcgcgt
     1861 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
     1921 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
     1981 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
     2041 aaacgaaggg gaaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
     2101 tggcccgcgg tttcagggaa tgagcaacac tttgcccggc atagcattac gagcgatgtc
     2161 acgcaccgcc tgggcaaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
     2221 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
     2281 ggagtagttc atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt
     2341 tgcagatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg
     2401 accggcaatc tctaccgcca gttcgacggt ttgcgggacg ccagccgtct cgaggataag
     2461 ctgattaaag cgcagctcgc gtaaaacgcc ctgtatttgc ggcgcgctca tttcacggct
     2521 gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc
     2581 gatagccgtc acactctttg ctcccagcgc gacagcgcac tgaattgcca gcaggccaat
     2641 ggttccggca ccaataataa taacgttttt attctcacat ccttgcgcta aatggaacgc
     2701 atgcaggcca acggtaatcg gttcaataaa agcgccatcc tcaataggca tatccgcagg
     2761 tagtgcaaag acattgtttc gcttaacgac aatatattcg gcaaatccac catcacgccg
     2821 tgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcagac actctggaca
     2881 agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccggggtgta aatcatcaac
     2941 accggatccc accgcatcaa tatagccact aaattcatgg cctaacgtta ttggataata
     3001 atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat
     3061 ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttctg ctacgcgcac
     3121 gataccatca gtatcattca ccactgattt cataaaaact cctgattgtt aagggggata
     3181 acctccccct caggtaaaaa ttattctgcg agaacggctt tctcttgttt aataaaacca
     3241 cgcgctctac gccaggtcat gaaaataccg gtaagataaa ttgcgccgat aacaatgaaa
     3301 ccgggaatat tttgcgggga gaaaacctga atcagtaacc aggtaatggg agaaccgccc
     3361 tgatccattg aagccaccat acccccggct tttaacgcgc ccgcattagc cgccagttgg
     3421 gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc
     3481 gagattaagg tgcggaacag atttccacga tgcaccgcga ccgccatcgc cacaaagaag
     3541 ccgatggtgg caaggtcgcc aaacggcagc acctgattac ccggcacaca aacagcaatt
     3601 aaaatggtga gtggaataaa aatcaggctt gccgatacca ccgccgtatg tcccagcagc
     3661 aacgccggat caaggccaat caggaactcc tgaccgccga acttcgcctg taaacgacta
     3721 cgggcctgct tagcgatggg cgttaaacca tccatgatgg gtttaatcac ccgtggcatc
     3781 agcagcataa ctgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc
     3841 gcgaggatgc cgataatcag ccccatcaca aaacccacgg tgaccggttc gccaaaaggg
     3901 ccaaatttgc gctgaatatc atcggcgcta aatttaattc ggttaacgcc tgggatttct
     3961 cgatgatagc atcgaccagc acagcaattg gccccatata cgccgacgta ccgtgcggaa
     4021 tagcaatgcc ttccagctca aagaaatcac gggtatcgcg ggcaaaccag tcgccgagct
     4081 tataaacaaa cgccgcgtga attaccacac ccgccatccc tatcatccat gaaccggttg
     4141 ccagatgcag caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta
     4201 ccacccgcgt catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg
     4261 ccaccagcgc aatttgcgac gcccaggtca tgggtgaaga gcccggccag ccgacatcga
     4321 ccacatgcag attcaggtcg aaattttccg ccatcgcttt cgccgccggg ccaatggaat
     4381 ccagcattaa gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg
     4441 ctttaaagca atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg
     4501 gcagcatcac cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc
     4561 acccctgtaa gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaacaa
     4621 aaggcatgcc gtgaactaac ggaatatcgc caaaactgcg ttcaactttg gcggtagtac
     4681 atatcaaatg cacgccatcc atataggttt ctatttcatt aacccgacac tggattaatt
     4741 caacaggaat attatgactc tgacacaact ctttaatttc ttccgccgcc atcgtagagg
     4801 tcgctaccgc gcctccgcaa gcgacaataa ttttgcgttt cataaatagt cctttttatt
     4861 gttacagagg gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact
     4921 ggggttcagg aagagtgatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata
     4981 aacagcgtaa aagtttcaat tgctgctgcg gattttccac aatcaacgca ataaccaacg
     5041 ataccgccac gtcgttatca tcatccgctt gctgaaaaag aactttattt gttggcctta
     5101 acagataaat ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt
     5161 gctgctcaag cattatcccg gtagggaatt ctgcttctct ggcaattaat gcctgtggcc
     5221 aggtatcatg aaccacacct ttcgcgagca tctcattacc gatatgggtt aaaacttcgc
     5281 tacgatcgac aaaagaaatt ccgccacgaa caaacaggtt agtcatatac cgtccttatt
     5341 ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat tttatccata ataagctgat
     5401 gcggtattgc tgataattcc ccggactgaa tgcgttcaaa ttgtttcgga agatactgac
     5461 taatcatgcc cagtgggatg tctacgcctt ccagattcac catcatcgtt tcgacgctat
     5521 ttttaatccg gctatgcggc cagtaataac gaatacgatc cgacaggctg taacgaatat
     5581 ccagtaatga atcgttaaaa cccgtgcgat aatatttctt ccagtactgc ggttcgtcga
     5641 gcatcacttc ttcaattacc gccaggcaac cactgctatt ttcgggggcg ataagttcct
     5701 gctcaatttg cgccagcgca aatatcgcct cgcgtaacgc aaaggttaat gccggaccga
     5761 ctttcaaaat tgcaaaatga tcgcgaacta attcccgata ggccgcctga gtctggtaat
     5821 cggtggaatg cgcttcataa accattttgg ttttttctat ccattgcgcc aacgcctgag
     5881 cttcttgcgc ctgataatga ataatattgc tatggtcaaa ttccacacca ggctgcacca
     5941 cgatggcaat cacgcgtgtt aacgcctctg ccagaccgcg ggcaataaac gctttttgat
     6001 gcgtgcgtaa agtattggcg gcatcttcaa cctgggtgat gtgtactgac tgaatggcgc
     6061 tggcctcacc gcccgggacc ggaacttcag tgccaatgac atagttcagt tgctcacgct
     6121 ggcaatcagt cgccacactt tccgccgcca gacaaagcac agcggcacgt tcagcaaccg
     6181 tttctggcgc taacggtatg gaatcgtcag cgcaggacat tgacgcatca agatgaattt
     6241 tactgaagcc ggcacgaaca tatgccttta ccagctcgac ggatttttcc atcgccgcat
     6301 ccgcattttc ttgctgccag cagtttggtc ccagatggtc gccgccgaga ataatacgct
     6361 cacgagcaaa tccgacttta tcggcaatag caaaaacaaa ttcgcgaaag tctgccggtg
     6421 tcattccggt ataaccgcca aattgattga cctggtttga cgttgcttca atcagcactt
     6481 tacgcgtgct gttgcgatca aatgccagcg ccgcttcgat aaccaacgga tgtgcagaac
     6541 agactgaaca tatgccaata tgttcaccag ctttatgccg ggcaattaac gttttcatgt
     6601 tttttccttg ttaaatggca ggtgcgttat gccctgccct cgcagccaca atcggcaatc
     6661 actttgctca ccacatcgcg cattgcagat ttagccgact gcaaataatc acggggatcg
     6721 gtcgcttcgg ggtgctcggt caggtaattt tttaacgcct gcgagaaggc atttttcagc
     6781 tccgttgcaa cgttgatttt gcatatcccc agtttgatgg tttgctgaat atccttagtc
     6841 gataatcctg acgcgccatg cagcaccagc ggtaaattca cccactggcg aatgttctcc
     6901 agtcgagaaa aatcaagcgc cggtgctctg gcatacatac cgtgagccgt gccgatggcg
     6961 accgccaggg aatcaatccc ggttgcctcg gcaaattcac gcgcctgaac ggggttggtg
     7021 tacaacgcat ctgcttcatt gacttgcaca tcatcttcct ggccgccaag ttgccccagc
     7081 tccgcttcga cgctgacatc aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa
     7141 atattttgcg caaaaggcaa atgcgaggcg tcaatcatga ctgagcgcac gccagaacga
     7201 accttctgag cgatatcgtc aaattttgtg tgatggtcga gatgaatcgc cagtggatgg
     7261 tgatattgct tcgccatcgc gctgaccagc gccatcagat tttctgtacc ggcatgagta
     7321 aatgtgccgg gcgttccggc gatgatgacc ggcgcatgca tgctggcagc ggtttccacc
     7381 accacttgca tcgtttcgag attgtgaata ttgaatgccg gaaccgcata accgccgcgc
     7441 tgcgcgttgt tcagcatctg ctttgtcgat accacgtaca ttttcacatc ctgtcatttg
     7501 ttttcgattt caaaatataa tgaaattatt tgttttcaaa tgtcgagata acgatcacaa
     7561 aaacgacaat gtgaaaatta atcagaatga caggcaaaaa aaacggcctc ccgggggaag
     7621 ccgtaacgaa gtgcgcgtgt ttttatgccg gatgcaacgt aaacgccttt tccgtcctac
     7681 ggggtggttc tgttcgtatg aggcgcatca ggcgatagta attttactat cgagataaac
     7741 gtcctgtacg gcgttaatca gtttcacgcc gtcagccatc gatttcttga acgccttacg
     7801 tccaagaatc agccccattc cgcctgcgcg tttgttgata accgcagtac gcactgcatc
     7861 gctgaggtca gtttcaccgc ctgcagcacc gccggagttt atcaacccgg cccggcccat
     7921 atagcagtta gctaactgat aacgcaccag gtcaatcggg ttttcactgg tcagtttgct
     7981 gtagactcga tcgtcggtat aaccgtaatt aatcgcttta tagccgccgt tattttccgc
     8041 cattttttgt ttgacgatat ctgcacctat ggttgccgcc agatggttcg cctgaccggt
     8101 caggtcggcg gaaacatggt aatcaacgcc atctttctta aaggcggagt tacgcaaata
     8161 ggcccacagc actgtcacca tgcccagctc gtgcgcacgt tcaaaagccg cagaactttc
     8221 ttcaatctgg cgacgtgact cttccgagcc aaaatagata gtcgcaccaa ccgccaccgc
     8281 gcccatgttg aacgcctgct ccacgctggc atacagcgtt tgatcgtagg tattcgggta
     8341 acttagcgtc tcgttgtgat taagtttgac gaggaatgga atgcgatgcg cgtaacgccg
     8401 cgataccgat gccagcacgc cgtaagttga cgccacacag ttacagcccg cttcgatcgc
     8461 cagttcaaca atgtttttcg ggtcaaagta gagcgggtta gcggcaaatg aagctccggc
     8521 agagtgctca acgccctggt caaccggcag aatagaaaga tatcctgtgc cagccagacg
     8581 cccggtgttg tacaacgtct gcatattacg taacaccgct ggcgggcgat tattgtcaat
     8641 catcacgcgg tctacgtagt catgtccggg gagataaagc tggtcagaag gaatagtcat
     8701 acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc aactgcgcaa tatctgtcat
     8761 tactatgctc ccgtaaattc cgattggata tcggctatgg attgtcctgg cccgcctttt
     8821 gcgggcaatc ataatcctgg tcgttacggg cgagtttttc catcttttgg atgttttttc
     8881 agcgttttct gttggctcga ttcatcagaa aaaatgttac cgcggtcaag ttttcaccgc
     8941 aaaggtattt aaaaggtatt actaagtggt attgtcatcg cgtaccttac attacctgtc
     9001 atgaaggaat taaaagatga aaacaacagc aaagctgtcg ttcatgatgt ttgttgaatg
     9061 gtttatctgg ggcgcgtggt ttgtgccatt gtggttgtgg ttaagtaaaa gcggttttag
     9121 tgccggagaa attggctggt cgtatgcctg taccgccatt gcggcgatcc tgtcgccaat
     9181 tctggttggc tccatcactg accgcttttt ctcggcgcag aaagtgctgg cggtattgat
     9241 gttcgctggc gcggtactga tgtatttcgc tgcgcaacag accacttttg ccgggttctt
     9301 cccgttactg ctggcctact cgctaaccta tatgccgacc attgcgctga ctaacagtat
     9361 cgcttttgcc aacgtgccgg atgtggagcg tgatttccca cgcattcgtg tgatgggcac
     9421 tatcggctgg attgcctctg gtctggcatg tggattcttg ccgcaaatgc tgggttatgc
     9481 cgatatctca ccgactaaca tcccgctact gattaccgcc ggaagttctg ctctgctcgg
     9541 tgtgtttgcg tttttcctgc ccgacacgcc gccaaaaagc accggcaaaa tggatattaa
     9601 agtcatgctc ggcctggatg cgctgatcct gctgcgcgat aaaaacttcc tcgtcttttt
     9661 cttctgttca ttcctgtttg cgatgccact ggcgttctat tacatctttg ccaacggtta
     9721 tctgaccgaa gttggcatga aaaacgccac tggctggatg acgctcggcc agttctctga
     9781 aatcttcttt atgctggcat tgccgttttt cactaaacgc tttggtatca aaaaggtatt
     9841 gttgcttggt ctggtcaccg ctgcgatccg ctatggcttc tttatttacg gtagtgcgga
     9901 tgaatatttc acctacgcgt tactgttcct cggtattttg ctgcacggcg taagttacga
     9961 tttttactac gttaccgctt acatctatgt cgataaaaaa gctcccgtgc atatgcgtac
    10021 cgccgcgcag gggctgatca cgctctgctg ccagggcttc ggcagtttgc tcggctatcg
    10081 tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag gaaccggtaa acggactgac
    10141 tttcaactgg gccgggatgt ggactttcgg cgcggtgatg attgccatta tcgccgtgct
    10201 gttcatgatt tttttccgcg aatccgacaa cgaaattacg gctatcaagg tcgatgatcg
    10261 cgatattgcg ttgacacaag gggaagttaa atgaaaacag aacgtattct cggtgctctt
    10321 tatgggcagg cgttagggga tgcgatgggg atgccctccg agctttggcc gcgcagtcgc
    10381 gtcaaagcac actttggctg gattgaccgt tttctgcctg ggccaaagga gaataacgcg
    10441 gcctgttatt ttaaccgcgc cgaattcacc gacgatacct cgatggcgct gtgtctggcg
    10501 gatgcgttac tggaacgtga aggcaagatc gatccggatc tgattgggcg taatattctc
    10561 ggctgggcgc tgcgtttcga cgcctttaac aaaaacgtac taggtccgac ctcgaagatt
    10621 gcgcttaacg ccattcgcga cggtaaaccc gttgctgaac tggaaaataa cggcgtgacc
    10681 aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc ttccggcgcg cgatgttgat
    10741 tcctttattg atgatgtggc gctggcctcc agcccgaccc ataaatccga tctggcggtt
    10801 gcgggggcgg tagtcatcgc atgggcgatt tctcgtgcca ttgacggaga aagctggtca
    10861 gcgattgttg attcactgcc ttcaattgcg cgacatgctc aacaaaaacg catcactacc
    10921 ttcagcgcct cactggcagc acgtctggag attgcgctga aaattgtgcg caatgccgat
    10981 ggcaccgaat ccgccagcga acagctttac caggtcgttg gcgcaggtac cagtactatt
    11041 gagtccgttc cgtgcgccat tgcgctggtt gaactggcac aaaccgaccc gaaccgttgc
    11101 gccgtcctgt gcgctaacct tggcggcgac acagacacca tcggtgctat ggcgacggca
    11161 atttgcggcg cgttgcatgg cgttaacgct atcgatcctg cgttaaaggc ggaactggat
    11221 gcagtaaatc agcttgattt caaccgctat gccacagcgc tggcgaaata tcgtcaacaa
    11281 cgggaggcga tatgagcggc gctcgattac acacgctgct gcctgaatta accacgcgtc
    11341 agccagtgat ggtcgtcggc gcggcggtca ttgatgtgat cgccgacgct tacgctctgc
    11401 cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt taacgttggc ggctgcgcac
    11461 tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc gggtaatgcc ctgccgctcg
    11521 gtcagggcgt gtgggcggag attattcgca accggatggc gaaagagggg ttaatcagcc
    11581 tgatcgataa cgctgaaggc gataacggct ggtgtctggc gctggttgag ccggatggcg
    11641 aacgcacttt tatgtcattc agtggtgtgg aaaatcagtg gaatcgcgaa tggctggcgc
    11701 gattaaccgt tgcgcctggc agcctgctct atttttccgg ttatcaactg gcctcgccct
    11761 gcggcgaatt gttagtggaa tggctggaag agctgcaaga cgtgacgcca tttatcgatt
    11821 ttggcccacg tattggcgat atcccggatg cattactggc gcggatcatg gcctgtcgac
    11881 ctttagtgtc actcaatcgt caagaggctg agattgccgc cgaacgtttt gctttatccg
    11941 cagagataac aacacttggc aagcaatggc aggagaaatt tgccgcgccg ttgatcgttc
    12001 gtctcgataa agagggcgca tggtatttca gcaacgacgc ttctggctgc attccggcat
    12061 ttccaacgca agttgtagac accattgggg cgggcgacag tcatgccggc ggcgtacttg
    12121 ccgggctggc ctctggtctg ccactggcgg atgccgtatt actgggcaat gcagtggcgt
    12181 cgtgggttgt cgggcatcgg ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg
    12241 cacacaaaaa cgtatagatc gctgcgacag tggctgatgc tgtactcaat aggccgttgc
    12301 tgttggtcaa gcgccacttg cttgatcacc agcaccggta ttttgctatc taactgaata
    12361 tgtgactgaa actcggcatc cggcatccgg gcgctaacgc gggaacgcgt acgttgtggg
    12421 taaatgtgct ggctgcggaa gtagtcataa agagaaatcc cgatggcatc aacatcatga
    12481 attaaatgtg ccggaaccca cgattcctca atcgataccg cctcttcatc gacatagcga
    12541 atacgcttaa gcaaaaaaac atcacttcct gcctcgaccg ccagctgctg cgcgacgtct
    12601 tcgggacatt tcacgacacg cttgttaacc catagcgtat cgggcttttt cccgcgcaac
    12661 accacctgct gagaaaaacc tcgcgcttct ttcaacgagt attcgaagat gttgttgatc
    12721 tgcgtgccgt aaccacgcga acgcgtcacc acgccctctt cttccagcga ctgcatcgcc
    12781 ttgcgcaccg taatgcgcga aacgccggtt aactgactta aatcacgctc accgggcaaa
    12841 atattgccat gctccagcac cccgctgcgc acggcatttt ttaccgtttc ggcaaactta
    12901 atataaagcg gcgtgttatc cgccgctaaa atacgctcgt tcagttgggc aatcagctgg
    12961 gtatgcgctt gttccatttg tctttttcct gccggggaat ctggtgggag tatatcactg
    13021 cttcttattt aaggcgtttc tttaatccca tcaacgaatc cctgcaactc ctcctctgtg
    13081 ccattaaaca cattaaaatc caccggacca ttaatgccat cgacctgtcc acggtcggaa
    13141 tgctgccaga agcgccaggc catgccgtca ttgtccgggc gacgttgata atagtgagcc
    13201 acccaccacg gatattcatt gaaatagccc gccagattgg tgtgataaaa aacggctcct
    13261 gagtaaataa tcggcttttt tcccgtacgt ttttcgacca tttttagcca ctgacttacc
    13321 cgcttgcgta attcttttgc cgataatttt cctcgctctt ctacgtccag taccgcaggg
    13381 aaatcgcctt gtgagaaatc caccgtttgc agaaataatc gcgcctgaac tgaagcggat
    13441 actgacgggg agaaataatg atacgccccg cgcagcaggc cattttcccg gcttagttgc
    13501 cagttacgcg aaaaataggg gtccaccagc ttttcgcctt ccgtcgcctt aataaaagca
    13561 aactgtaagc ggataccgtt gtcgcgcatt tttgccaccc gctgccagtc gatccgctcc
    13621 tgccagcgcg aaacatctat tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa
    13681 gatttaaccg ggcgataacc gtagaagtgg atgtagtctt taatctgaat tgctgtcgaa
    13741 tagaaaaaat tcaccgtctg acgcggataa attgcaacga tggaaattag cccaagagcg
    13801 cataataaaa cggtgaattt ttttcgactg gtgattctca actgcattgg tttcatccct
    13861 gaatgtcagc accaaaggct gacaataacc aaagcagcta tagtacggtg ctcaccacca
    13921 ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact tccagcgtgt cggcctgggc
    13981 taatgccgat gaaagccagc tttttgcctc ctgtacggtg tcagcccagt ttgtatggcg
    14041 cgggcgcagt gcagccaacg ccgcagagag cgtacaacca gtgccgtgag tgtttttggt
    14101 cataatgcgc tgtgcggtaa accgttgttc accctcgcgg gtaaacagcc agtccgggct
    14161 ttgctcatca tccagatgac cacctttcat tagcactgcg ccacagccca tcgccaacag
    14221 cgatcgccct tgttccagca tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc
    14281 ggctgcttcg ggcaagtttg gcgttattaa tgaaacctgt ggcaataatc gactgcgtag
    14341 cgtggcaact gccgaaggtg aaagcagcgg gtcgccgctt tttgccagca taacggtgtc
    14401 gagtaccacg ttttggatct gataacgttg caaccgttct gccaccgctt caacaatatc
    14461 ggtttccgcc aacataccga ttttagtggt gtcgattcgc acatcgctga acaccgaatc
    14521 gagctgggcg gcgacaaaat caggctcaat gcgatacacc gactgtacac cacgggtatt
    14581 ttgcgccacc agtgcagtaa taactgagca accataagcg ccaagtgccg agaaggtttt
    14641 aagatcggcc tgaatccccg caccaccact cggatcagta ccggcaatcg tcagagcgtt
    14701 aattcgtttc atgcctgcac ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga
    14761 acaaaactgc ctggcccctc gcttctggcg actgcgcgtt ctccggcttg tttcatccag
    14821 tgacaggcag atgcgatatt ttccagcgta tcgcctggta gcgcacaaca ggcagcgaca
    14881 accgccgata gtgcacagcc agttcctacc actttggtca ttaacggatc gccaccgtga
    14941 atgccaatga tacgatgtcc atcggtaaca taatccacct cgccagtgac cacgacgatt
    15001 gcgccagttt cccgtgccag tgtttgtgca gcaggtatcg cgttagctgc ggcatcagtg
    15061 gtatccactc cccgtccacc attagcaacg ccagctgatg ccatgatttc cgaagcatta
    15121 ccacgtatgg cggttggctt atgggacaga agttccagac aaaaacggcg gcgataatcg
    15181 agcgcaccaa ccgcaaccgg atcaagcgtc cagggcgttt gtgatcgtgt tgcttgctca
    15241 acggcagcac tcatcgcttg cgctcgtggc cgcgtcagcg tgccaacgtt aatcaacaag
    15301 gcactggcga tagccgcaaa ctgactggcc tcttcggttt cgataaccat cgctggcgat
    15361 gcgccgagcg ccagcaaggt attggcagta aaagtttgca ccacatcgtt ggtcatgcag
    15421 tgcacaagag gggaatgttg gtgaaaaagg tgtaacgcgt gcgcagattg cgctgaactc
    15481 agcaggtcga cttgcatagt ttgctcctgc cataacgtga agaagcaatg acctggtggt
    15541 ccgtgacttc cctacgctgg cattatccag atcaggtgat acgggtattt ctcagccttc
    15601 acaaagaagg gcaccccgag tcgtttggtt gcgataataa atcgcagagg aggatggtaa
    15661 tgtccagcgc acgcgttgta aacggggaaa aacggcggta ttacaataac gttgtgaaat
    15721 tatttgatat aagaatccag caccttcaga acgacatcca gatcttcttc acgttttagc
    15781 tcatccccct ggtgaacgat gtgttccgtc agatggcctt taatcacttc ccgcatcaga
    15841 ccgtttaccg cgccacggat agcagcaatc tgttgtaaaa ctgcagcgca ttcatgcggt
    15901 tcgtcgagca ttttcttgag cgccacgacc tggccctgaa tcttactggc acgcgctttc
    15961 agtttctgtt tatcacggat tgtatgagac atggcaacac ctggttaaca agaatatgaa
    16021 aaatcatagc actattaatc tactgggggg tagtatcagg tactgggggg gagtagaatc
    16081 agattgccga attaatacta agaattatta tcatgaccga atttacaaca cttcttcagc
    16141 aaggaaacgc ctggttcttc atccccagcg ccatcttact tggtgcgctt catggtctgg
    16201 aaccggggca ctcaaaaacg atgatggcgg cgtttatcat cgccatcaaa ggcaccatta
    16261 aacaggcggt gatgctcgga ctggcagcaa ctatttcgca taccgcagtg gtctggttaa
    16321 ttgcctttgg cgggatggtg atcagcaaac gctttactgc tcaatcagca gaaccgtggc
    16381 tccagttgat ttccgcagtg atcattatta gcaccgcgtt ctggatgttc tggcgtacct
    16441 ggcgcggcga acgcaactgg ctggagaata tgcacgagca tgatcatgag catcatcacc
    16501 acgatcacga agatcaccac gaccatggac atcatcacca tcacgaacat ggcgagtatc
    16561 aggatgccca tgcacgagcc catgccaatg acattaaacg acgctttgat ggtagagagg
    16621 tcaccaactg gcaaattttg ctatttggct taaccggtgg ccttatcccc tgcccggcag
    16681 caattaccgt gctgttgatt tgcattcagt tgaaagccct gacactgggc acaacactgg
    16741 tcgtcagttt cagcctcggc ctggcgttaa cgcttgtcac cgtaggcgtt ggcgcagcaa
    16801 tcagcgttca gcaggtcgca aaacgctgga gcggatttaa cactctcgct aaacgcgctc
    16861 cctatttttc cagtctgttg attggcttag ttggtgtgta tatgggcgta catggcttca
    16921 tgggcataat gcgataactc aatcctttag gcttgcatgt tatcttcagc ccaggatgat
    16981 tcttaaatca gctatttccg ctgactctct gctcgctaag gatgctttta gggcatcctt
    17041 tcatttacac tttttacgaa atcatgggat cactaacaaa atatcgcttg tcagttatat
    17101 tgtatggcag gaaagatatg cgactgatat tacagatccc caaagtggag agtttatgac
    17161 cattaaaaat aagatgttgc tgggtgcgct tttgctggtt accagtgccg cctgggccgc
    17221 accagccacc gcgggttcga ccaatacctc gggaatttct aagtatgagt taagcagttt
    17281 cattgctgac tttaagcatt tcaaaccagg tgacaccgtc ccagagatgt accgcaccga
    17341 tgagtacaac attaagcagt ggcagttacg taacctgccc gcgcctgatg ccgggacgca
    17401 ctggacctat atgggtggcg cgtacgtgtt gatcagcgac accgacggta aaatcattaa
    17461 agcctacgac ggtgagattt tttatcatcg ctaaaaaaaa gccccctcgg agagaggggg
    17521 gaatgcagac accttaatat ttttattatt agccacttgc tcgtcttgct tgttattagt
    17581 cgtatttcac gttgattaat gcggttgcct ccagtgcgcc agatttaact ttgtttgtat
    17641 cgtagacgta gtaactggcc gttatcggaa ttgaaaccgt tgtatctgta agttttgact
    17701 ttgccgcaat ttctt
//
LOCUS       DAPQZD010000096        17704 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPQZD010000096 DAPQZD010000000
VERSION     DAPQZD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.096"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            309..1151
                     /locus_tag="RO454_003116"
     CDS             309..1151
                     /locus_tag="RO454_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDY0934911.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEALIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(1210..2187)
                     /locus_tag="RO454_003117"
     CDS             complement(1210..2187)
                     /locus_tag="RO454_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HDY0934912.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRELQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAVRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQILLQAVNGQARISSQTVRNLLG"
     gene            complement(2204..3133)
                     /gene="flgL"
                     /locus_tag="RO454_003118"
     CDS             complement(2204..3133)
                     /gene="flgL"
                     /locus_tag="RO454_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDY0934913.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLVVRDDLLEAANGTN
                     TADSLASLGQDIESLTDSMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(3148..4524)
                     /gene="flgK"
                     /locus_tag="RO454_003119"
     CDS             complement(3148..4524)
                     /gene="flgK"
                     /locus_tag="RO454_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDY0934914.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(4714..5013)
                     /locus_tag="RO454_003120"
     CDS             complement(4714..5013)
                     /locus_tag="RO454_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HDY0934915.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMLVKQLSPRHK"
     gene            complement(5013..6113)
                     /locus_tag="RO454_003121"
     CDS             complement(5013..6113)
                     /locus_tag="RO454_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDY0934916.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMTATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(6128..6793)
                     /gene="flgH"
                     /locus_tag="RO454_003122"
     CDS             complement(6128..6793)
                     /gene="flgH"
                     /locus_tag="RO454_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDY0934917.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(6876..7661)
                     /gene="flgG"
                     /locus_tag="RO454_003123"
     CDS             complement(6876..7661)
                     /gene="flgG"
                     /locus_tag="RO454_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDY0934918.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(7740..8477)
                     /locus_tag="RO454_003124"
     CDS             complement(7740..8477)
                     /locus_tag="RO454_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HDY0934919.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(8477..9679)
                     /gene="flgE"
                     /locus_tag="RO454_003125"
     CDS             complement(8477..9679)
                     /gene="flgE"
                     /locus_tag="RO454_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HDY0934920.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(9951..10664)
                     /locus_tag="RO454_003126"
     CDS             complement(9951..10664)
                     /locus_tag="RO454_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HDY0934921.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(10664..11095)
                     /gene="flgC"
                     /locus_tag="RO454_003127"
     CDS             complement(10664..11095)
                     /gene="flgC"
                     /locus_tag="RO454_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDY0934922.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(11098..11433)
                     /gene="flgB"
                     /locus_tag="RO454_003128"
     CDS             complement(11098..11433)
                     /gene="flgB"
                     /locus_tag="RO454_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDY0934923.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            11515..12252
                     /gene="flgA"
                     /locus_tag="RO454_003129"
     CDS             11515..12252
                     /gene="flgA"
                     /locus_tag="RO454_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDY0934924.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGETV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            12334..12612
                     /gene="flgM"
                     /locus_tag="RO454_003130"
     CDS             12334..12612
                     /gene="flgM"
                     /locus_tag="RO454_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDY0934925.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            12625..13053
                     /locus_tag="RO454_003131"
     CDS             12625..13053
                     /locus_tag="RO454_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDY0934926.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRAREDL
                     LAVNDEITQHYEQLHAHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            13181..14113
                     /locus_tag="RO454_003132"
     CDS             13181..14113
                     /locus_tag="RO454_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HDY0934927.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRQSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQAILSTLTLPDLTPELYLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQPARQLMTLLG
                     SCAFDWSILPESPRKTSLPLQMPLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRESENAVLIRQQIQAERSADPLLYAFSLLSC"
     gene            14160..16598
                     /locus_tag="RO454_003133"
     CDS             14160..16598
                     /locus_tag="RO454_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDY0934928.1"
                     /translation="MQTLIKTLVTHRVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAVLTDIVSDPLLRNAYLGLHNATHYQAIHHQAIDQVVLINLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLHTDKWLACYPSLCYQRQGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSHKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWCAVQGQLAEIRHHYCDAFMDGK
                     AGERAAMVIMEALG"
     gene            17080..17481
                     /locus_tag="RO454_003134"
     CDS             17080..17481
                     /locus_tag="RO454_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934929.1"
                     /translation="MLQKIGYMEEDNIIEKKPWRGIPPEYTDRWREIYNNAAPEEGMH
                     LSDACPVCGDTELYRYYSLLKTQPRLLGGIMYKGPGSYWEWCGSCRSYEHMSGYVPIS
                     WVVELSGIDHSKLTAIPDMIDIARHKNHYFS"
BASE COUNT         3926 a         4908 c         4708 g         4162 t
ORIGIN      
        1 tggccaggta tcaaaatatg tctttatcaa atgcttgata tagttaagat tgtcatcaat
       61 taacgcgttt tcttctcaat gtactgatgc caaaatttac attgcgttgc aaatagtaca
      121 cgtttggcta ttgtacctgt cctgtcattt cgttacattt ccacgccata tagtgattaa
      181 gaataatcct attaccataa aatattaatt atcacgcaga aaaatcatct tctcagggac
      241 gtataagact gtgctttatg caaagcacta tcaggaaaca tttatccctt gctaaggtaa
      301 actcaactat gagtattatt atcaacaact ggcggatgga cccgtcgctt aatgccttaa
      361 tacattgcga aacgggtgaa acacgtcgtc tcggcgaata ccattttatt ttgctggaaa
      421 cattagcaaa aaatgccgat gtggttttat cgcgatctta tttatgtgcc gaagtatgga
      481 agaaccgcat agtcggcggt aacagcctgc cgacggcgat ccatgcgttg cgcgttgcta
      541 tcgatgatga tggcaaacaa cagaacatta ttaaaacaat tccgaagaaa gggtacctgt
      601 gcaataaaga gtatgtgtct tttccggagt catcgcctgc ggaagcgctg attattaccg
      661 accagataca gaaaacaacg ccagaagaga tatcttcgac aacaccgcct gtacctgtta
      721 gaaaaaaaca taaaggactg atggggctgg cgttggccgc ggctgttata ttggttggct
      781 caaccgtggg gtattcacac ttgaaaagta cacctgacgc cccgcaactg gtaaaagagt
      841 caattaacag tccaagaata aaaatatttc atcttagttc tggaaaggaa aataattccg
      901 taccgttact gtcacaaatc ctcgcaccag gaaaagacaa actcgataat ttattgtcag
      961 cgcataatat gacaatgaca acctattata aatatgttcg taaccgactg gaaagcgata
     1021 ttgttctgcg taaccagtgc aacggcagct ggcaattaac ctttaatgtg gaaggttggc
     1081 agaacagcga tattaatagc gcgatgtatc agaatttaga gaagttgtta aatactgtgc
     1141 agaaatgctg acaagtgcca atatatgccg gatgcgacgc taaacgcgtc ttatccggcc
     1201 aaccgataat tatcccaata aattcctcac cgtctggctg gaaattcgcg cctgaccgtt
     1261 caccgcctgc aataaaatct gatagttatg attggcgcta tccagcacgc cgccgaggtt
     1321 ctccgatgcc tgcaccgcgt tttccgtttg cgggaagcgt gacatgccgt cgatcaactg
     1381 gcgggctttt tcctgctgca ccgccatctg cgcgcgctgc tgattaatgc tttctaacgt
     1441 ctgggaaatc cccgccccac cctgttgcaa actttgcgct aaacgcacgg cctgcgacgg
     1501 ttcggcaaag gtttttagtg gcataaacgc cgacttatca ccatcggtgc gcacgcttaa
     1561 ggtgcgctcg atgtcaggcc actgtttttc ggtggtggag aaactgatgc cgtccgcttg
     1621 ctgatgtacc tgcacgccca cgcggcgcag ggcgttggtc aggcgcgtct ggtactgccc
     1681 ggcgctgtca tcctcagaca gcatcaccgc cgaaagctgg gtctgccgcc cgtcagagac
     1741 actgaacatg cgcgttcccg gcgtgggatt gtgtaccaga ttcgccagat ccggcgagtg
     1801 gaaggtcacc cgcgcttcgc cctgcaacac tggttgtaac tcgcggtcca ccgcgccgcc
     1861 ggaaagcgcg gcgcgtttgt ccagcatctg catcagcgcc gtactctgcg cctgcccgcc
     1921 cttacgctgg ctgtgacgat agtcgagcaa ctgctgttcc agttgcccga gatagtgatc
     1981 cgcctgctgc aacgtggtga gctggtcgtt aagctgtacg ctgtaacgct gcgggcgagt
     2041 ggtaatcagc ggcgaggccg gatattcact cgccgtcgcc gggagttttt gccgcaccgc
     2101 ctgagtggac gcaacggttg acgacgatac cgcagcgcta tgtgcgccgc cggtggcgag
     2161 tgatgaagat gtaagtccga cctgcatact gttcctgaaa gagttagaga acgctgaaca
     2221 aattcaactc actcactttc gagtaagtct tgagggtagc ttccattgcc acttccagtc
     2281 cggtaaaggt gatcgacgcc ggaccgtaat cgagatcctg caatgaacca atcaattcat
     2341 cattagatgt cgagatatcg gtctgtgcat cgctcaacat cgacatggtg ttttgtgtct
     2401 cgccgagcga cgcaatcgag gcgttgaggt cgtccgaggc ggtgtccacc acatcgacgg
     2461 cgttctgaat atcgctctgc acctgcggat ctgccgggtc gacgtccggg ttttgcagtt
     2521 cctgcgacaa ggaattaagc gtgttaagca cgtctaaatt gctaccgaaa aaatcgctcg
     2581 ccgccacgtt ggtgtccact tccacaccgt tcgacacggt agtctggcga tggtcgctgt
     2641 tgccctgata gctatagctg tcggtgacgc catcgccatc atcgtctacc gcgacaatcg
     2701 gcggctgatc gttgatggtg ccgccaaaca cgtagtgccc gtcttcgtcc tgataattga
     2761 gcgccgccac cattgaatcg gtgagcgact caatatcctg gccgaggctt gccagcgaat
     2821 cggcggtatt ggtgccgttc gccgcttcaa gcaggtcgtc gcgtaccacc agcaggctgt
     2881 tgttgacgcc atcgagaatc gattcctgct ggcttaagcc cgccgacgcg gagtcgatat
     2941 tgctctgata ctgttcaatc gccgactgct ggcggtttaa ctgggtgatg cgcgtggcgg
     3001 cgattgggtc atccgacggc tgcaaaattt ccttgccggt tgccatctgc tcgaccacat
     3061 gcgccagaga gccggagaga ttattaaaac tttgcgtcat cgagacgtag gtttgttggg
     3121 tggttactcg cattttcgcg ctcccgctta gctgcacatt tccagcaccg aatcgaaaat
     3181 ctcggccccg gcggaaatga ctttcagatt agcttcataa atttgttgat aggtgatcag
     3241 gttcaccgct tcttcgtcca tgctgacgcc gctgacgcta ctctgctggt tttgcgctgc
     3301 ggaatagaca ttggacgcgg catcgacttc cgtctgattt tgctgactgt aaatgccgat
     3361 attgctgatg attgacgagc acgcctgccc gaccgtcaca ctgccgaggt tggctatctc
     3421 cagcggttcg gtggagatat tgatcagcgc ctgcaggttg tcgctgttgc cgctttcgtc
     3481 cggtgaactg gaaaacgcca gctcatcggc agtgatatcc gggttaacgg tcagtgggcc
     3541 atcggcattg ctggcgtcat aaataaacag tggctcgccg gggttaccgt tgagatcgta
     3601 gccctgcgcc agctggttgt tgaccgcatc ggcgaactgc gacgccatgc tgttgatggt
     3661 gtcggtcagc ggcgtcagca cgtcgttttg atagtcgaac agagcgccta acgaaccgcc
     3721 agtgtcggtg gtcatggtcg aggtggtgcc agcgaaagtc agcgacatgg tcggtgtgcc
     3781 gtcggcgttg gtttccagcg cgatggtcga gctttgctgc ccgctcacca gcggctgacc
     3841 gtttttcagg gtgacgttgt agttgccctg gtcgtcaata ttgacctgca cgtccatcat
     3901 tccgctcagt tcttccacca tctgatcgcg ggcgtcgtac agcgcggagg cgttatcgcc
     3961 gttggcttcg gcctgagcga tttgctggtt atagctggcg atgccgctgg tcagcgtatt
     4021 gatttgcgat accatcgcct gctgctgact gatgatttcc gtgctttgcg agtcgatgta
     4081 atccagcgtg ttgtcgatgc gcaacgacag cgccccggct tcgctgatca cctgctcgcg
     4141 cagggcggaa tcatcggggc tggtggtcgc ttcgttaagg gcagcgaaaa agttatcgaa
     4201 gccgccgctc agactgctgt tatcgtcgct cagtaccgct tccagttgtg tgagataccc
     4261 ttgttgggtg ctgtaatagc cgtaatcgct ggcggcatac catacctgat taacctgata
     4321 ctggttggag acgcggcgaa tactgtcgac ctgcacgccg ttaccagcgc tgttcggcga
     4381 accaccgctg gcaccgatgg tgctgatctc cgccacctgg cgggtgtagc ccgtggtcat
     4441 ggcgttagcg gtgttttgcg ccgtgacgtt cagctccacc tgcgccgttg atgcgccgct
     4501 gtagccgatg ttaatcatgt ccatgttggg tccttaccgg taaatcgtgc cggttaaaag
     4561 cggcgtgaag ttggggattc ttaagttccg taaaccgggt gccaaatgcc ggatgcggcg
     4621 taaacgcctt atccggccta catactacat acgggcgact cgtaggcctg ataagacgcg
     4681 acagcgtcgc atcaggcacc gaacgccgaa gcctcattta tgcctgggcg acaactgctt
     4741 cacaagcatc gccgcaatcc caatcccgtg ctgctgcgcc agggtgttac acaactcatc
     4801 gtcataaaac ccctgcatca tgcgcaccga atcgctgtta aacgggttat ctttcgaatc
     4861 aaacaactcg ttggttttac gcatctcttt cagcatgttg cgtaaaaaaa tcgcctcaaa
     4921 ctgctccgcg gcctgcttaa tatcattggc ctgaaccttc ggccccatta acgcatcgct
     4981 gccgtcaatc ccgccgctgg cgttcacttt catcagatca cctccaggtc ggcatccagc
     5041 gcgcccgcct catgcagcgc ctgtaaaatc gacataatgt cgtccggcgt tgcgcctaaa
     5101 ctgttcagcg cattgaccag cgttttcagg ctgctcgact ccggcaaact caccacgccc
     5161 gggcgcgcat gattcaccgc gatattgctc tgcggcgtta ccgccgtgcg cccgcccgca
     5221 aaagcatttg gctggctgac gttgctggtt tcgttaatcg agacggtcaa actgccgtgc
     5281 gacaccgccg ccgcatgcag cgcaacgcca tcgcccatca ccaccgtgcc ggtacgcgag
     5341 ttaaacacca cgcgggcgcg gattttctct gcctgcacct gtacatcatc cagttgcgac
     5401 ataaatgcca cccgcgcgcc ggggctggtg ggcgcacgca cgctgacgtt ggtagagctc
     5461 tgcgcagtag caatgccgcc aaacgaggcg ttaatcgccg ccgcaatatt gttggcatct
     5521 ttaaacgacg gacgtttcag gttgagggtg atagtgtcgc ccatctgaaa atcgctgggg
     5581 atctcgcgct ctaccgttgc gccgttagga attaacccgg cggttggcgt attaaccgtt
     5641 acgctcgaac cgctggcccc ggtggcagtc atcccgccca ccaccacgct gccctgcgcc
     5701 agcgcataaa cttcgccatc cgcgccgtgc aactgggtca gcaacaaagt gccgccacga
     5761 atacttttgg catcgccaat cgacgaaaca gtgacatcga tggtctgccc gcgcgagtac
     5821 atcggcggca gcgtggcgct caccgctacc gcggcgacgt ttttcacctt cggatcgatt
     5881 ttatttggca gttgcacgcc aaactggcgc agcatattgg tgatggtctg gttggtgaat
     5941 ttgacctggt ttttatcacc agtaccgtcg aggcctacca ccaggctgta gccgaccagt
     6001 tggttttcac gcacgccctg tacgttgacc aacgactcca gcgattgcgc cagcgccacg
     6061 ccgggcagcg tcagacttac agcgatcgct gcggccttta tccagttttg cattgcactc
     6121 tccggcatta aatcgggaac agaggatggt taaacagccg cgtcaaccag cctgcggcgt
     6181 tggcgtcgct taacgcgccg cgtccggcat aggagatgcg ggcatcggcg atccgctgcg
     6241 aggaaaccga gttgtcgttt tggatgtcgt cggcgcgcac caggccgctt aagcggatgt
     6301 actcatcgcc ctggttgaga gtcagccatt tttcgccgcg aatttccagg atgccgttag
     6361 gctgcaccgc atggacggaa acggtgatct ccccgcgcag gctgttctgc tgctgggaag
     6421 tggcgctgcc gtcaaagtcg cggttggcgt cgatagagcc ggaaagttta tctttggtgt
     6481 gtccaaaaat cgtgggcgcg ccgatatcga cggtgttgct tttgccgaag ttggttttcg
     6541 cctgtttgct cgactgggtg gactcttcga ggatcaccgt caggatatcg cccacgcggt
     6601 aagcccgccg gtcggcggtg agcgaccagt tgtagccggt ttcaaacacg cccccggcac
     6661 gcccgtctgc cggaggcggc gcttcggtgg ttggtggcgc aaagtatgcg tcgtcttttt
     6721 tgaccagaat ggcctgcgat tcacacccgc taaggagtgg aagcagcgcc accagccaga
     6781 gataattttt cacgttctgc ctgtttaaat acagtgagtt atgtgctctt gtaggtcgga
     6841 taagacgcgt cagcgtcgca tccgacattt cggccttaca gcgtctggct gatgtactgc
     6901 aacatatcgt ccgcagccga gaccattttg gcgttcatct cgtacgcccg ttgcaccgta
     6961 atcatcgcca ccatctcgtt gacgatatcc acgttcgatc cttccagcgc gttgtcctgc
     7021 aaggtgccaa gcccatcttc gcccggtacg ccttcggtcg gctgaccgct ggcggcggtt
     7081 tccagataga ggttgtcacc ctcagcggaa agcccggccg ggttggcgaa gttcaccagc
     7141 gtcagttgcc ctaactcagt cacgtcgctg tcgccgggta agatcgccgt caccgtgcca
     7201 tcctcaccaa aagcaacatt agtcgccccc gccggaacgt cgatttccgg ctgcaacggc
     7261 aagccctgcg agttggtcag tacgccgtca gcgttcacct gcaagttgcc gtcgcgggta
     7321 taagcgatat cgccgttggc cttttgcacc tgcaaaaacc cctgccccat aatggcgaca
     7381 ttcatcgcgt tgtcggtggt ttcgacgttg ccttcggtga aggttttctg agtgccgaca
     7441 atgcgtacgc cgctaccgaa ttgcagcccg gtcggcataa tgttgttctg atcgagcatc
     7501 gcgcccggcg cttcctgggt ctggtaaaac agatcctgaa acatcacccg gtcacgcttg
     7561 aagccggtgg tgttaacgtt ggcgatgttg ttggcaatcg cgctcatttc agcatcctgc
     7621 gccgagaggc cggttttgct gatccatagt gctgcgttca tggtggtaaa ttccgtgtta
     7681 ttttttggcc gcggttgccg gatgcggcgt gaacgcctta tccggcctac gatgtgcgat
     7741 tacgagccgc gcaataagcg gttgcccgcg tcgctgatat cttcggcggt tttcatcatc
     7801 ttgatctgcg cttcgaactg gcggttcatg gcgatggacg acatcatctc gctcaccgcc
     7861 gagacgttgc tgccctcgag aaaaccgccg ctgactttga tgttttcatc gcgctgggcc
     7921 ggaacgccgt cggcagtcac cagcatccct tcaggatttt tcgccaggtt cgcgaccggg
     7981 atatccacca gcttcaggcg gtcgatatcc atcgtcgcgg tcacgtcacc gtcatcgggc
     8041 gtcacggaga gcgtgccgtc gctgccaaac gaagcaatgg cgttgggcgg cagaatgatc
     8101 ggcccgttat ctcccagcac cagattgccg tcgatagtca gttgcccctg gtcatcctgc
     8161 tgaatgttgc cgttgcgggt atacacttcg ttgccgtttt tatcctgcac agcaatgtaa
     8221 cccgaaccct gaatcgccac gtccagtggg cgttcggttt tctccgcaac accagtgctg
     8281 tcgttcacgc cgctggcccc ttcctgcgcc atatagcgcg tgtcgaagcc gccgcctttc
     8341 accttatcgt tagtcgccat tgccatatcg gcgcgaaaac cgttggtgtt gacgtttgcc
     8401 aggttgttgg cgctgatctg ctgctcgtac agggtctgag aagccccgct cagggcggta
     8461 taaatcaagc ggtccattac attgcctgga acagcgcgtc atcgagctgc gtgctggtgg
     8521 cgataacttt ggtgttggcc tgatagttac gctgggcggt catcaggttg actaactcgc
     8581 tggtgatatc gacgttagag ctttcgagca cgcccgacga cagcgtgccg caggtgccgg
     8641 aaccgggaac gccaatcagc ggggtgccgg attcgccggt ttgtacccac gcggtgccgc
     8701 tcactgcctc caggccgttt tcattcggga acgtcgccag caccacctgc ccctgcaaca
     8761 tgcgctcgcc gttgctgtag gtagcgtaaa ctttgccatc gtcatcaacc tgcacaccgt
     8821 tctgagtggc agaggcgtag ccgtcggcgg cgttggtggt gaccgaaaat tcagagccgt
     8881 attgcgtaca ggtcgagtaa tcgacggtta aatcgatggg tgcggcggcg tcggtctgaa
     8941 actcaatggt ctgcggcgtg gttggcgagg tcagtttccc ggtgttcggg tcgaaggtta
     9001 acgtcgtcgc cggaacgccg gtctgctgct gaccgtcgaa ggtgtactgc acttcccagg
     9061 tgttgtcgct ggttttggtg aagtactggc ataccgaatg ttcgttgcct aatgagtcat
     9121 acaccgtggt ggtgtagctg tcggtgtacg agctgctgtt ggtcgcgtcg aacggtacgg
     9181 tagtgcggtc gatagccgca tcgctggcat cgaagttggc ggtaaaggtc aggctgctgc
     9241 tggcctgcgc cggaatatta ccggttttga tctggatatc ggtgactgta ccggtttgca
     9301 gggtgcccgt gtcatcgacc ggatagccct gcaaataagc gcccgaggcg ttgacgatat
     9361 agccgttttt gtcggtttca aacgatccgg cgcgggtata agaaatgttg cccgcgctgt
     9421 cgcaggtaac aaaaaagccg tcgtcgttaa tcgccagatc cagcgcgtta ccggtggata
     9481 ccagcgaacc gccgcgcgaa atactttgcg cggtgcctgc cacgctgacg cccatcgcct
     9541 gacttccggc atacatggct gaaaactggg tggtcatcga cttatagcct accgttccgg
     9601 cgttggcgat gttgttactg atcccgtcca gctgttcgtt aacggcattc agccccgttg
     9661 cagcaatttc ataactcatt gtgtcttcct gttaattcag attttggcag cgtgcccgtt
     9721 gttttgccgg atgcggcgtg aacgccttat ccggcctacg aacgggtgca aacataaaaa
     9781 tatgcgcgaa ctcgtaggcc tgataagacg cgttaagcgt cgcatcaggc atcttgcacc
     9841 gaacgccgga tgcggcgtga acgccttatc cggcctacaa aaccattcgg attcaatagt
     9901 ttcatgtagg cctgataagc gaagcgcatc aggcaatttg gcaccggaca tcacgccaca
     9961 tcactgtcgg catcgggtac gccaaactgg ctaatcatgg tgaacggcac ttcgcctaca
    10021 ccggcaacgt taagcaccgg ggagctgccg tcgagcggga tgcgcacatc ctccacttcg
    10081 ccggaaactt caatcggcac ctcttcttcc ccggtgttgg tgaccaccga gacgtcgtaa
    10141 tcgcccggcg ggataccgta atccgccgga ttaatcgaga aatccacctg acctggcccg
    10201 acggacgaac tgccttccgg gatcagcgac accgtatagt catcgcctgc ggcatcggta
    10261 aagtggagat cggcggtggt acaggcgtcg tcaagcgtgg cgcgaccatt aatggtctgg
    10321 tcgctcacct gcaaggcggt ggtctgcacc ataatgtcgt cacccaccag gttgccgagc
    10381 gccatcacct gaatgttgct catcagcacc gcgttggtgt tggcctgcac gctcatctct
    10441 tccatcatcg ccacctgcgc catcgaagaa agctggtcga tatattcggt ggcgtcagtc
    10501 gggtcggtcg ggtcctggtt ttggatctca gcgacaaaca gcgtcaggaa ggtatcgacg
    10561 ggcgagctat cgctgctact ttgcgtgtcg ctggccgcca cgttgttgct ttccgccgtg
    10621 gcggtcggct gcggtgattg cgcattcagc gccagggtat tcatcaggct tctcccagtt
    10681 tcagcaaact ttgctgcata cttttcacgt tgttgagcac atcgacattg gtttcgaaat
    10741 cgcgcgaagc cgacatcatg tccgccatct gttccaccac gttgacgtcg ggataccaga
    10801 catccccgtt agcatcggcg agcggcgaat gtggttcata gcgtttcaca gctccgccgg
    10861 tttgcagcac gtcctgcacc tgcacactgg caccgtcaat ggcatgacga tgtgtacccg
    10921 ccagcaaact gtggtgatac accgccgcaa acaccgggct gcgcgcttta tatgcctgcg
    10981 cctcgctgct ggcgggcgca ttggcattcg ccaggttact ggcaacggta ttcagacgca
    11041 gcgtttgcgc cgtcatcgcc gaaccggaaa tctgatagat atcagtaaat gacatgctta
    11101 tttcccttca atggcctctc tgatgccgct gatttgcaga ttcagaaagg tcagactgct
    11161 ttgataatcc atactatttt gtgaaaaccg cgcttgttcg ctgttcagtt ccacggtgtt
    11221 gccatcttcc gacggctgca tcggcacacg gtactggaca tccaccgccg cgttatttgc
    11281 ccgttgcatc tccctggcaa aatcaatatc tttagccttg aaatttggcg tatcgacgtt
    11341 cgccagattc gcagtcagta gctcggtcct ctcaagacgt aacttcactg cctgcggatg
    11401 tactcccaat gcctgctgaa aactgatccc catttcctgc gtcactcctg cttcaatccg
    11461 catacgctaa gcaatcgccg tgccattttt taagtgactg aagagaaagg gtttatgagt
    11521 tttcaatttt gcaggcggaa gttttatttc ctcctggcgc tggccttacc gggctacgcc
    11581 gccgtccatc ccgtgcagca cagcgcgcgg gaacaagtca acgcccaggt actgaatgcc
    11641 gccagccaaa aaattgaatc gctggcgcaa cagcggcgct ggcatgacta tcgctacacc
    11701 tttaaggtct acattccctc gcagattgcg accgccgcgc cctgtactaa aacacccggc
    11761 gttacgctga cttcgcctgg ggacatcgcc ctcaaccgga tgaacttcac cgtcagttgc
    11821 ccacaaagct ggcagatgaa cgtagcggtg cgtccggatg tactggtgcc agtagtaatg
    11881 gcgaagtcac tggttgcccg cgatacgccg ctcaccgcga atgatgtgga gttgaagcct
    11941 tacaacgtca gtgcccagcg ccgggaggtg ttgatggaac ccaatgacgc gattggtttc
    12001 agcagtaaac acgcgctgca accgggcagg ccaatcacca aagaagaact gatatctccg
    12061 gtgctggtcg agcgcgatca accggtgatg attgtttacc agagcgcggg gatcaccgcc
    12121 tcaatgcccg gcgtagcact gaaaaatggt cgcaaagggg aaacggtgaa ggtgcgtaac
    12181 gccagcagcc agcgggtcat cagcgcgatg gtggcggaaa gcggcgtggt gacaacggta
    12241 agcgcggagt aatttgcgcg ggcgattaaa gttttgccgc ctgttaaccg ctttaccgga
    12301 gtaacccacc caaaataccg agacgaggtt acgatgaaaa tcaccccgac tatgccaggc
    12361 aatcacccga cagccacaat ggcaggctcc agtcagccgc gcaaaagcac ggcgaccacc
    12421 gccacgacgc tgagcgccga tgacattacc caggcgggat tgcagtctgc ccagcaaacg
    12481 ctgaacgacc agcaagagag cgatatcgac agcgacaaag tggcacaaat gcaggcgatg
    12541 ctagcctccg gcagcctgca agtggacacc gagcagttag ccagcgacat gctgagtttt
    12601 ttccagaact aagaggccga tgaagtgagt atccggttac aacaggtgaa ggcgttgttg
    12661 cagggaattc gtgacgacga cgcactgtac gattccctgc gtgaacttct ccagcgtcag
    12721 cggatctgca tgattcgccg cgccagagag gatctgctgg cggtaaacga cgagataacg
    12781 cagcactatg agcagttgca tgcgcatagc catcagcggc atagcctgct gaaaatgctc
    12841 ggtgttagcg taaatcgtga cggtctggcg caggtttttg cctggcttcc ggcggtacaa
    12901 aaagcggcag cgcaacagtt gtggcaacgc cttgagcaaa aagcagaacg ctgcaaagcc
    12961 tataacgata aaaacggcga gctgttaatt cgccagtatg aatttattca ggctttttta
    13021 ggcagcgaag cggattttct ctatcaggga taacgacaat tccttccccc atcgcggaaa
    13081 cggattttcc gcctgatccc caatcctttg tttttgatta aaaaaattcc cggcacgctt
    13141 attgcacatc ctgcaaggta ataaagactg gaaactacct atgagtttta ttgaatgtta
    13201 cgaaccggaa tatgtgcgca attttatggc acgccacccc gattcgcctt tatacgtccg
    13261 caaggtgtgg aaaaacgaga ttcgccagag cctgatgctg acagagccag aaagctgcga
    13321 agcggtgctc aacgatgcct gcgcctttga tttacagctc acctaccgcc cggtggaaga
    13381 aaatgccgca gaactctccg cgcgcgatgc gatagtaaac caggccattt tgagcaccct
    13441 tacgctcccc gatttaaccc cggagcttta cctgtatgcc gtcggaattg tgctctcccg
    13501 cgccagtaaa atgccgggca gagatggaga tattcttgcc cgcttgacga cgctgccaca
    13561 ggcgctggcc gatcatgcac aaaaaggcac gctacaggca cagttcgccc agctcccgcc
    13621 cgtgccacag ccggcgcgcc agttgatgac gctgctcggc agttgtgcat ttgactggtc
    13681 gattttgccg gaaagcccgc gcaaaacttc cctgccgttg caaatgcctt tgctgacgct
    13741 acatgacgcc aacagcaaag ccctgttaca acagcagctt aaggtgcagt ggcaaaccac
    13801 ctggcaacag cattttgcca ccgcgccgtg gatgatgcgt aactggctga tttaccgcgt
    13861 ttaccatgag gtgattggac aggcagacgg cgcagattat tgcccgctgg tgtgcgattt
    13921 ttatcttatc cgcacgttaa tcagtttatg gacgctggat ggttctccgc tgcgacagga
    13981 ggatattttc gccctcttcg ccatgtttga acgctggcga gagagtgaaa atgccgtgct
    14041 gattcgccaa caaattcagg ctgaacgttc cgcagatccc ctgctttatg cgttttcact
    14101 gctgtcttgc taacgctttc tggccggttt tcccggcctt atcgacttcg taaactatca
    14161 tgcaaaccct catcaaaaca ctcgtcactc accgcgtaaa accgtttgtc agcgtcgtca
    14221 cgccaacctg gaaccgcggc gcattcctgc cgtacctgct ttatatgtac cgctatcagg
    14281 actacccggc agaccgccgc gaactcatca tcctcgacga ctccccgcaa agccatcaac
    14341 acatcatcga ccgcctgacc aatggcacgc cggaatcctt caacatccgc tacatccatc
    14401 acccggaaaa actgccgctg ggcaaaaaac gcaatatgct caacgaactg gcgcgcggcg
    14461 aatacatcct ctgcatggac gatgacgact actactcgcc agataaaatc tcttactcca
    14521 tcgccatgat gcaaaaacac cgggcgctca tctccggttc cgaccagatc cccatctggt
    14581 acagccacat caaccgcatt ttcaaaagcc gcagctttgg cccacataac atcctcaatg
    14641 gcaccttttg ctatcaccgt aactacctga aaaagcaccg ttacgacgac gattgtaatt
    14701 taggcgagga gaaaagtttc accgacaatt tcagcgttaa cccgctgcaa ctgcccggcg
    14761 agcgcacgat tctgtgtatc tcgcacagcc acaacacctt tgataaagac tttatcctcg
    14821 gggccagcac gcctgtaaac gcggtgctta cggatatcgt cagcgatccg ctgctacgca
    14881 acgcttatct gggtctgcat aacgccactc attatcaggc gatccaccat caggcgatcg
    14941 atcaggtcgt attgatcaat ctggataaac gcccggatcg cttacagcag atccgtgaag
    15001 aactgacatt gctgcatatt ccgcctgaga agatcacccg ccttgccgcc agtgaaaatg
    15061 aaaacggtca acggggccgc cagcaatcac acttgcaggc gctgcgtctg gcgcaacagc
    15121 acggctggca aaactacctg ttgctggaag atgacgcggt tattctcaaa caggagaaac
    15181 atattcaggt gctgaacgcg ctgctggcga gtctggcgaa aattccctgg caggtgatga
    15241 tcctcggcgg cgaaatttcg cagggaacga tgctaaaaag tttgcctggg ctggtgcacg
    15301 cgcgggactg ccgcaaagtg tgcgcttatc tggtgaacag ccgctactac ccgcagctgg
    15361 cgcagcagat gaacaacgat gagcattcgc tggaagacgg ctggcagccg ctgctgcaca
    15421 ccgacaaatg gctggcctgt tacccgagcc tttgttatca gcgacagggc tttagtgata
    15481 tcgagaaaaa agcgactgat aacattaacc actacttcaa taagttaccc gtagccacca
    15541 agccatctac attacctatc gccgatacca tcggcttttt tatggaaacc tcattccact
    15601 acgcgctcta ccgcccaatc atcaccgccc tgcaggcgca ggggcagagc tgtacgctgg
    15661 tgatcaatga ccgcgtattt aaaccatttc tggatgaaat gctggcgacg ctaaaaagca
    15721 tcgacgatcc gcagctcaaa ggaatgcgct tatctgaaat gcaggcacac ggtcagcgag
    15781 tgaaatgcct ggtcagcccc tatcacacgc ccgcgctcaa tggcctttca accctgaaca
    15841 ttcgcgccat gtacggctta gccaaagaaa cgtggaacca cgccgactgg aaccgttttt
    15901 atcagcgcat tttgtgttac agccactaca gccagcgggc gctggcacat ttcggcagcg
    15961 caaaagtggt cggcaatccg cgcttcgacg cctggcataa cggcacgttt gaccgcgctc
    16021 tgccggaaaa tatccaaccc gattcccata aacctacggt gctctacgcc cccacgtttg
    16081 gcgcattaag ctccctgccc cactgggcag aaaaattggg ccgcttaagc ggtgatgtga
    16141 acctgatgtg caaactgcac cacggcactt gctcgcgccc ggaagaagct gcatcgctgg
    16201 cactggcgcg caggcattta aaacagcgca ccgactctgc ccgccacacg ctggcgctac
    16261 tggcaaaggc cgattacgtg ctgaccgata acagcggctt tattttcgat gctattcacg
    16321 ttgataagcg ggtgatcctg ctcgactttc cgggcatgac aacgttgctc gacggtgaga
    16381 aaagttattc cacgcccgaa agtgccgacc agcaaattcg cgaaatattg ccggtggctc
    16441 atgatgttgc ggaattacgt tatctgctat cagaggcgtt tgactggtgt gcggtacagg
    16501 ggcaactggc ggaaattcgt catcactatt gcgatgcgtt tatggatggc aaggcgggag
    16561 aacgggcggc gatggtgatt atggaggcgc tggggtaaac cgcgcaagtg cctgatgcgc
    16621 ttcgcttatc aggcctacgc gcccctgcaa tatagtgaat ttgcgatgat ttgtcggccg
    16681 gaaaaggcgt ttacgccgca tccggcacga acagtgcact tatctaccct tcgatttcaa
    16741 atacatcgct ttatggctgg ttaatcggta aaagttgcac attttgatag cgagaagagc
    16801 tgtctttatg aattccgaaa agcgcccttc taccctctat aaccattctt ttttacctgt
    16861 attggccgca acatacctca cccacgaagt ggttgtagca attcgtgggc taaaggtccg
    16921 cattatatga aacgggaggg cggtagcatt tctgatatta catgcctcaa ttttgctgaa
    16981 ctattggcta tcctggacct caaaaatctg gtcataagat agatttttta agggaatacg
    17041 gagtaaaggt gtggaaaact aattaatagg tttgtcttaa tgcttcagaa gattggatac
    17101 atggaagaag ataatattat agagaaaaaa ccatggcgtg ggatcccacc tgaatacacg
    17161 gatagatgga gggaaattta taacaacgct gctcccgaag aagggatgca tttatcagat
    17221 gcttgtccag tatgtggtga tacggaatta tatcgctatt attctctgtt aaaaacgcaa
    17281 ccacgcttat taggagggat tatgtataaa ggacctggaa gttattggga atggtgtggg
    17341 tcttgtcgtt cctatgaaca tatgtcaggc tatgtaccaa tatcgtgggt tgtggagtta
    17401 tcaggaatag atcattcaaa attaacggcc attccagata tgattgatat tgcaagacat
    17461 aaaaatcatt acttctctta aatattatct aattagtttt cccggacagg gcaagttggc
    17521 gaaaatagtg aggagcatca cgttttccat cctgactgac acgcctactg gtgattaaaa
    17581 tcaaacctat ggaatgacga aaaagatgga aaggaaaagc atatcaagta atcagtcagg
    17641 aagatattca caacaatcac ctgaatcagt agatcgaatg aaggatcact tgtcacataa
    17701 tata
//
LOCUS       DAPQZD010000097        17577 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPQZD010000097 DAPQZD010000000
VERSION     DAPQZD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.097"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            104..955
                     /locus_tag="RO454_003135"
     CDS             104..955
                     /locus_tag="RO454_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDY0934930.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGFPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            948..1319
                     /locus_tag="RO454_003136"
     CDS             948..1319
                     /locus_tag="RO454_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDY0934931.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1316..2068
                     /gene="fliP"
                     /locus_tag="RO454_003137"
     CDS             1316..2068
                     /gene="fliP"
                     /locus_tag="RO454_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDY0934932.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2071..2343
                     /gene="fliQ"
                     /locus_tag="RO454_003138"
     CDS             2071..2343
                     /gene="fliQ"
                     /locus_tag="RO454_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HDY0934933.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2345..3127
                     /gene="fliR"
                     /locus_tag="RO454_003139"
     CDS             2345..3127
                     /gene="fliR"
                     /locus_tag="RO454_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HDY0934934.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHNLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3117..4256
                     /gene="flhB"
                     /locus_tag="RO454_003140"
     CDS             3117..4256
                     /gene="flhB"
                     /locus_tag="RO454_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDY0934935.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLSLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4240..6333
                     /gene="flhA"
                     /locus_tag="RO454_003141"
     CDS             4240..6333
                     /gene="flhA"
                     /locus_tag="RO454_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDY0934936.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(6553..7050)
                     /gene="rayT"
                     /locus_tag="RO454_003142"
     CDS             complement(6553..7050)
                     /gene="rayT"
                     /locus_tag="RO454_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDY0934937.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTQDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAEDVTDFSAG
                     ERIIL"
     gene            complement(7226..7975)
                     /locus_tag="RO454_003143"
     CDS             complement(7226..7975)
                     /locus_tag="RO454_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDY0934938.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            8287..9027
                     /locus_tag="RO454_003144"
     CDS             8287..9027
                     /locus_tag="RO454_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDY0934939.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(8998..9765)
                     /locus_tag="RO454_003145"
     CDS             complement(8998..9765)
                     /locus_tag="RO454_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDY0934940.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(9970..10548)
                     /gene="lpcA"
                     /locus_tag="RO454_003146"
     CDS             complement(9970..10548)
                     /gene="lpcA"
                     /locus_tag="RO454_003146"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDY0934941.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            10788..13232
                     /gene="fadE"
                     /locus_tag="RO454_003147"
     CDS             10788..13232
                     /gene="fadE"
                     /locus_tag="RO454_003147"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDY0934942.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(13275..13721)
                     /gene="ivy"
                     /locus_tag="RO454_003148"
     CDS             complement(13275..13721)
                     /gene="ivy"
                     /locus_tag="RO454_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDY0934943.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            13902..14672
                     /gene="yafV"
                     /locus_tag="RO454_003149"
     CDS             13902..14672
                     /gene="yafV"
                     /locus_tag="RO454_003149"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDY0934944.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            complement(14771..14847)
                     /locus_tag="RO454_003150"
     tRNA            complement(14771..14847)
                     /locus_tag="RO454_003150"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14811..14813),aa:Asp,seq:gtc)
     gene            complement(14980..15711)
                     /gene="dnaQ"
                     /locus_tag="RO454_003151"
     CDS             complement(14980..15711)
                     /gene="dnaQ"
                     /locus_tag="RO454_003151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDY0934945.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            15776..16243
                     /gene="rnhA"
                     /locus_tag="RO454_003152"
     CDS             15776..16243
                     /gene="rnhA"
                     /locus_tag="RO454_003152"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDY0934946.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(16240..16962)
                     /locus_tag="RO454_003153"
     CDS             complement(16240..16962)
                     /locus_tag="RO454_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0934947.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            16996..>17577
                     /gene="gloB"
                     /locus_tag="RO454_003154"
     CDS             16996..>17577
                     /gene="gloB"
                     /locus_tag="RO454_003154"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDY0934948.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKE"
BASE COUNT         4126 a         4523 c         4696 g         4232 t
ORIGIN      
        1 atttgcccct ttcactcccc ctctatttaa tacctcacct tatgtcaccg ccggatttgc
       61 gtgcacagaa acgcccatac cattacagca gagaaagtaa gccatgctga agtacagtaa
      121 aacgccaggc atctttaaat tagaaggtaa tcggcttgga cgcccctacc atcgcctgcc
      181 gaccctgttt acggggaatt ttgatgtaat tgattcgcat cttggcagct actttctcaa
      241 aaagcatcgc agcaatatca ccctaaaaaa gattggctgc gaaatggata ttattaataa
      301 aaatgccgag ctgatggttt cccaggtcgg gcatctggcg tttgatattg atcgctcctt
      361 gctgttaatg ctgctgggca atttttatgg tctggagtcg tctcttgaag aagcgaaatc
      421 acacaatgga ttcccgacca aaacggaaac acgtttaaaa aaccgcctcg cgctggatat
      481 ctgcacgctg atttttaatc tgcaaacctc cggtattgcg ctgaaattaa aactggacag
      541 cagcacggta attacccact gggcgtatca attaaccttt acccttgcgg gcgatgaaga
      601 gagcagtttt cgtattttgc tcgacgacgc ccacaccgat tttattttga atctgatccg
      661 ccacagcgag cacagtaaac cgcagcaggt ggcgaaatcc gttaataaac cggccctgat
      721 taaagaaatc atccgctccc tgccgctgac cctgaacgtc aaaatcgccg agctatcgat
      781 gaatgtcgcg gatctggcac agattaaagc cggggatatt ttacccatat cactgggaga
      841 aacgttcccg gtggcgattg ggcaatctga attatttagc gccctgattg tggaagacaa
      901 agacaaactg tttttgtcgg agctggcggg aaaaaatgag aacaaccatg agtaagcaag
      961 aagatatttt agccggggat tttggcctca ccgatgaagt tgcgcctgtc gccaaatccg
     1021 ccgacgccga aacgctggtc acccgcctgg aagaccgatt ttccgactcg atgacgctac
     1081 tgaaacgcat tccggtgacc ttaacgctgg aagtatcgtc ggtggaaatc atgttagccg
     1141 acctgcttaa catcgacgac gacacggtga ttgaactgga taaactcgcc ggggagccgc
     1201 tggatattaa agtgaacaat atcctgctgg gcaaagcgga agtggtggtg gtcaacgaga
     1261 agtacggcct gcgcgtgctg gaatttaaca cccgcgatat caacgacctg gcgccatgaa
     1321 acgccgcact caactgacgc tgggcctggc gctgctggcg ctcgccccgc tggcgctggc
     1381 ccagggcggc gatatcgcac tgcttaacgt ggtcacccac ggcaacactc aggagtacag
     1441 cgtcaaaatt caggtactga ttctgatgac cctcgtcggt ttgctgccga cgatggtgct
     1501 gatgatgacc tgtttcacgc gttttattat cgtgctgtca ctgctgcgcc aggcgctggg
     1561 gctgcaacaa acgccgccaa accgcattct gattggtatt gcgctctcac tcaccctgct
     1621 ggtaatgcgc cccatctggc tcaatattta cgaccacgcg gtagtgccgt tcgaaaacga
     1681 tcagattacc ctgaccgatg cgttaagcac cgccgcgacg ccgctaaaac gctttatgct
     1741 ggcgcagacc gacaaaaaag cgatggcgca aatcatgacc atcggcggcg cgaagggcaa
     1801 tgccgccgac caggatctct ccatcgtggt gcccgcctat gtactgagcg agctgaaaac
     1861 cgccttccag attggcttta tgatttacat cccgtttctg gttatcgacc tgattgtcgc
     1921 cagcgtattg atggcgatgg ggatgatgat gctgtcgccg ctgattgttt cgctaccctt
     1981 taaactgatg ctgtttgtgc tgattgacgg ctggtcgctg accatcggca cgctcaccac
     2041 cagcattcgc gggctggggc tgggctgatt atgttgaccg tagacgtcgc cgccgacatt
     2101 gtcgccagcg gcattaaagt agtgattttg ctggtttcgg tactggtggt gccaagcctg
     2161 ctggtcggcc tgctggtgag cgtgttccag gcggtgacgc agattaacga acagaccctg
     2221 agctttttgc caaggctgat tgtcacgctg gtggtgctgg gcgtgtgcgg caaatggatg
     2281 attatccagt tgcatgatct ctgcattcac ctgtttaccc aggccgcgtt gctggttcat
     2341 tgagatgcgc acaagcgacg ttacgcaact gatggatctg gcgctggggc tgtggtttcc
     2401 cttcgtgcgc attatggcgt ttctgcgcta tatgccggtg ctggataaca gcgccctgac
     2461 cgcgcgcgtg cggattattt tgtcgctggc gctggcgatc atcatcacgc cgttgatccc
     2521 ccatcccatt ccccacaatc tgctatcgct caacagcctg atcctgacgg tggagcaaat
     2581 tttatggggc atgttattcg ggctgatgtt tcagtttttg tttctggcgc tacaactggc
     2641 cgggcaaatt ctctcgttca atatggggat gagcatggcg gtgatgaacg acccgagcag
     2701 cggcgcatcg accaccgtgc tggcggagct aatcaacgtc tacgcgatcc tgctgttttt
     2761 tgccatggac gggcatctgc tgctggtaag cgtgttgtac aagggcttca cctactggcc
     2821 gattggcaac gccctgcatc cgcaaaccct gcgtaccatc gcgctggcct ttagctgggt
     2881 gctggcgtcg gcatcgctgc tggcgctgcc gaccactttt atcatgctga ttgtgcaggg
     2941 ctgctttggc ttgctcaacc gtattgcgcc gccgctgaac cttttttctc ttggctttcc
     3001 gatcaacatg ctggcggggc tggtctgttt tgccaccctg ctctacaacc tgccggatca
     3061 ctatctgcat ctggcgaatt ttgtcttaca acaactcgac gcgctgaaag gtcactatgg
     3121 cggatagcag cagcgaagaa aaaacagaga aaccctcggc gcaaaagctg cgtaaagcgc
     3181 gtgaagaggg gcaactgccg cgctcgaagg atatggggct ggcggcgagc ctgtttgcgg
     3241 cgtttgtggt gatctccagc agttttccgt ggtatgccga ttttgtgcgc gagagcttta
     3301 tcagcgtgca tcagtatgcg caggagatta ataacccgca ggtgatcggc cagtttctgc
     3361 gccatcacct gttaattctc ggcaagttta tcctcacctt gctgccgatg cctgctgccg
     3421 cgctgctctc ttcgctggtg cccggcggct ggctgtttct gccaaaaaaa atcctcccgg
     3481 atttcagcaa aatcagtccg ttaaaaggca ttgggcggct gttttccagt gaacatctgg
     3541 cggaaacggg aaagatgacg gtgaagtctg tggtggtgct ggtgatgctg tgggtaagcc
     3601 tgcgcaataa ctttgccgcc tttctcagcc tgcaggcgct gccgttcaaa ctggcgataa
     3661 atgacggttt atcgctctac gccagcgtga tgcgcaactt tgtcattctg tttatcttct
     3721 tcgcgcttat cgacgtgccg ctggcgaaag ccctgttcac caaagggctg aagatgacca
     3781 aacaggagct gaaagaagag tacaaaaatc aggaaggtaa gccggaagtg aaggcgcgtg
     3841 tgcggcgact gcaacggcaa ctggcgatgg ggcaaatccg caaagtggtg ccgaaagcca
     3901 acgtggtgat caccaacccg acccactacg ccgtggcgtt acagtatgac cagtcgcgcg
     3961 ccgccgcgcc gtttgtggtg gcaaaaggca ccgatgaaat cgccctctat attcgccagg
     4021 tcgccacgga gaatcaggtg gaagtggtcg agttcccgcg gctggcacgc tcggtttact
     4081 acaccaccca ggtgaaccag caaattccgt ttcagcttta ccgggccatc gcccacgttc
     4141 tgacctacgt cctgcaaatg aaacactggc gcgagggcgc acagccgcgc ccggccctga
     4201 acagacatat ttccattccc aaagaggtgc ttaaactgga tggcgaaaac aactaaatcg
     4261 ttccttgcgc tgctgcgcgg cggcaatctc ggcgtgccgc tggtcatact gtgtattctg
     4321 gcgatggtta ttctgccgct gccgcctgcg ctgctggata ttctgttcac cttcaacatt
     4381 gtgctggcgg tgatggtact gctggtggcg gtgtcggcga aacgaccgct ggaattcagc
     4441 ctgttcccga ccattttgct gatcaccacc ttaatgcgcc tgacgcttaa cgtcgcctct
     4501 acgcgcgtgg tgctgttgca cgggcatctc ggcgcgggcg cggcgggtaa ggtgatagag
     4561 tcgtttggtc aggtggtgat cggcggcaac tttgtcgtcg gtttcgtggt gtttatcatc
     4621 ctgatgatca tcaactttat cgtcgtcacc aaaggggccg agcgtatttc cgaggtttct
     4681 gcccgcttta ccctggacgc gatgcccggc aaacagatgg caattgacgc cgatcttaat
     4741 gccgggttga tcaaccaggc acaggcgcag acccggcgca aagatgttgc cagcgaggcc
     4801 gatttttacg gcgcgatgga cggggcatcg aagtttgtgc gcggggatgc catcgccggg
     4861 atgatgattc tggcgatcaa cctgatcggc ggcgtctgta ttgggatctt caaatacaac
     4921 ctgagcgccg atgctgcctt ccagcaatat gtgttgatga ccatcggcga cggcctggtg
     4981 gcgcagatcc cttccctgct gctctccacc gcggcggcga ttatcgtcac ccgcgtcagc
     5041 gacaacggcg atatcgccca tgacgtgcgt aatcagttgc tggcaagccc gtcggttctc
     5101 tacaccgcca ctggaattat gttcgtgctg gcggtggtgc cgggaatgcc gcatttcccg
     5161 ttcctgatgt tcagcgccct gcttggtttt accggctggc ggatgagcaa gcgcccgcaa
     5221 gcggcggagg cggaagagaa aagcctcgaa acgctaaccc gtacgatgac agaaaccagc
     5281 gagcaacagg tcagttggga aactattccg ctgatcgaac ctatcagttt aagcctcggc
     5341 tacaaactgg tggcgctggt ggataaagcg cagggtaatc cgctcaccca gcggattcgc
     5401 ggtgtacggc aggtgatatc cgacggtaac ggagttctgc tgccggagat ccgtattcgg
     5461 gaaaacttcc gcctcaagcc cagccagtac gccattttca tcaacggcat taaggctgat
     5521 gaagcggata ttccggcgga taaactgatg gccctgccct ccagcgaaac ctacggcgag
     5581 attgacggcg tactggggaa tgacccggcg tacgggatgc cggtcacctg gattcaaccc
     5641 gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc cagcgtgatt
     5701 gccacccatg tgaataagat tgtgcgcagc tatattcctg acctctttaa ctatgacgat
     5761 attacgcagc tgcataaccg tttgtcgtcg atggcaccgc gactggcgga agatttgagc
     5821 gcggcactca attacagcca gttgctgaaa gtgtaccggg cgctactgac tgaaggcgtt
     5881 tccctgcgcg atatcgtcac catcgccacc gtgctggtcg ccagtagtgc ggtgaccaaa
     5941 gatcatattc tgctggcggc agatgtgcgc ctggcgctgc ggcgcagcat tacccatccg
     6001 ttcgttcgca agcaggagct gacggtgtat acgctgaata atgagctgga aaatctgctg
     6061 accaacgtgg tgaatcaggc gcaacagggc gggaaagtga tgcttgatag cgtgccagtg
     6121 gacccgaata tgctcaacca gttccagagc acgatgcctc aggtgaagga gcagatgaaa
     6181 gcggcgggga aagatccggt gctgctggtg ccgccgcaac tgcgcccctt gctggcgcgc
     6241 tacgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt gccggatgag
     6301 ctggagttga agataatggg ggcgttgatg taggcatccg gcatcggtgc atattgccgg
     6361 atgcggcgtg aacgccttat ccggcctacg ggtgagcacg gttttgtcgg catgataaga
     6421 cgcgtagcgg cgcatctggc accggtgcac aactgccgga tgcggcgtga acgccttatc
     6481 cggcctacgg gtgagcgcgg ttttgtcggc atgataagac gcatctgcgt cgcatcaggc
     6541 atcggcgcac agctataaaa tgatacgctc cccagcacta aaatccgtta cgtcctccgc
     6601 ccaatcgatg ggatataacc ctcgcgcgac atcgcgatga aacgttgaga atggccaatc
     6661 actcacttgc tttacccaac catgttttac tggatttata taaatataat caacatgatg
     6721 gcgatagtct tgggtattgc gaatggcatg ctcccaaaaa cgcggttgcc agatattttt
     6781 caatccacaa gcatgggtaa attgcttttt aatttcccgc cagcgcgagg aaaaatcatc
     6841 atcgccttca ggtaatgtcc atatgcaatg catatgctcc ggcaaaacta cccaagcgtt
     6901 aatttcaaaa ggtctgtctc gcttaacgtt aataatagcg ttacggagcg tctgaaactg
     6961 ggtggtcaaa agttggtttc gacgattctg taaatttacc gtgaaaaacc aggttccccc
     7021 cttgatgtaa taacgacgat attcagacat aacgactcct ttttctcaac ggtaagaaga
     7081 aaaggaaaag tcggcaatga tgttgtgtta aatcgggaaa gcggctcgca aaaaatgcac
     7141 agatttatag gcatgataag acgcgtagcg tcgcatcagg caccggtaca caaccgccgg
     7201 atgcggcgtg aacgccttat ccggcctaca aaatcgtctc ttccgtcaaa atccgccgcg
     7261 cgcccaaaaa atgatcctgc cagaaaggtt cagctaaccg acttacccga atggtttccc
     7321 cggtgcgcgg tgactcgata aactgaccgt ctccaagata tacgcccata tggtcggcta
     7381 tctcacggct gtggatatgg aaaaacagca aatctccccg gcgcaggtcg ttattcgcca
     7441 caatcgttgc ccgacggtag tggtacatct cattggccgt gcgcgggagc ttagcctcaa
     7501 ggatcttgtt gtaggcataa aaaaccaacc cgctacagtc gaaaccttta tcaggccgcg
     7561 tgccccccca gacataaggc ttgccaagtt gctgttccag gcggtgaatc gccacttcgg
     7621 tgatgttatg caggtggttg ctgctgagga aatgattgtt ttccgccagc gcctgccagc
     7681 gtctgtcgtt cgccttgagc ggcgcaggaa accattctgg atgctgtttt atctgctccc
     7741 gattatgctg gcgcagcgcc cttctgcttt tagcattgcc ttccacaata tagctggcct
     7801 gctttttcag atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc
     7861 gggcggcgta gctaaaagag atatgtgatg tggtggcgct ggcgagtaaa gggagcaaaa
     7921 cgcataacga aaacacgcct ggatgcagaa atctgccgtg caagaacgaa gacatgaagg
     7981 acataaacca gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat
     8041 taattgctta tttgcatatt actattgcgg tttcataatc ttaggaaata agaaataaat
     8101 ctacaatcca tgcgcttttc ggactcttga aaactgcacc tgtgctaaat ttgtaaaccc
     8161 tgcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
     8221 tattccgatt tgggattcat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata
     8281 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccatg cgtttcgttt
     8341 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
     8401 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac
     8461 gtcaaaatgg gcgagcaata tcaactgctc gacagctata aaatctgtaa atattccggc
     8521 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
     8581 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattccca
     8641 aacgcctatg accgtgcgca tggttatgaa gggaaatacc tgatgattca cggcgattgt
     8701 gtttccatcg gctgctacgc gatgaccaat cagggtattg atgagatctt ccagttcgtt
     8761 actggtgcgc tggtgtttgg tcagccgagc gtgcaagtga gtatttatcc gttccgcatg
     8821 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcaactg
     8881 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat
     8941 ggccgttacg tggtcagcaa gccgttaagc cacgaagttg tgcagccaca actggcatca
     9001 aactacacgc tccccgaggc aaaataagcg ccattcacct ggcataatct tttgccaggt
     9061 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
     9121 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
     9181 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtcagaaag
     9241 cagcatgttg aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa
     9301 cactgccgcc atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca
     9361 gcaaaaggct ttttcgctat cggtttcacc gaccgggcgg aagttgccgg tttccagtga
     9421 tttgtagccc gtcagttgtc cattatgggc ataagtccag ttacgccccc ataactcgcg
     9481 ggtaaacggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
     9541 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
     9601 gctgggttgc ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
     9661 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
     9721 gatatcggta gggacgttgg cactcatccc gagcaattcg cacatgggta acctccacaa
     9781 cagcgggagc gcatggcccc cggcgaagat tgatttaaga attttcaggt cggatgtggc
     9841 gtgaacgcat tattcggcct acggttcggg tgcagattgt aggcatgata agacgcgtca
     9901 gcgtcgcatc aggcatcaga gcacaaatgc cggatgcggc gtaaacgcct tatccggcct
     9961 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
    10021 aatgtggatc tcctgaatgc ggtcggcata accaaagtgc ggtacgcgaa tttcgatatc
    10081 cgccgtgcca gccattttgc cgccgtcttt accggtcagg gtgatcactt tcatcccctt
    10141 ctcacgcgcc gccgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
    10201 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtaa cgggagaaaa tatcattgaa
    10261 accaaaatcg ttaccgacgc aggaaatatg actaacgtca gaaatggcaa tcgccgggta
    10321 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tggcgtcgca
    10381 atgggagccg ccgttgccac aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
    10441 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
    10501 cgtttccgcc gcttcgttca gttcattacg aataagatcc tggtacatga ggatatcctt
    10561 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
    10621 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
    10681 agctttgttg taattatatt gtaaacgcat tgctaaatgt ttttacatcc actacaacca
    10741 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
    10801 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
    10861 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgcgggt ctgtggtcgg
    10921 cgtgggtact agtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
    10981 agtcgatgat ttccgcaccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
    11041 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
    11101 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gctgaagagc
    11161 aagcgtttct cgacggtccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
    11221 ctcatgagct ggcggacctg ccgccggagt tgtgggcgta ccttaaagag catcgtttct
    11281 tcgcgatgat catcaaaaaa gagtacggcg ggctggagtt ctcggcttat gcccagtctc
    11341 gtgtgctgca aaaactctcc ggcgtgagcg ggatcctggc aattaccgtc ggcgtgccga
    11401 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaaaatcact
    11461 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgacc agccccgaag
    11521 cgggttccga tgcgggcgcg attccggata ccgggattgt ctgcatgggc gaatggcagg
    11581 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga
    11641 ttgcgaccgt gctgggtctg gcgtttaaac tctccgaccc ggagaaatta ctcggcggtg
    11701 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
    11761 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
    11821 tgttcgtgcc gatcgattac atcatcggcg ggccgaaaat ggccgggcaa ggctggcgga
    11881 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
    11941 gcgtgaaatc ggtagcgctg gcaacgggtg cgtatgcgca cattcgccgt cagttcaaaa
    12001 tttctattgg taagatggaa gggattgaag agccgctggc gcgtattgcc gggaacgcct
    12061 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggt gaaaaacccg
    12121 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcaa tcgattatcg
    12181 acgcaatgga tatcaccggg ggtaaaggca ttatgctcgg gcagagcaac ttcctggccc
    12241 gtgcttacca gggggcaccg attgccatca ccgttgaagg ggcaaatatt ctgacccgta
    12301 gcatgatgat cttcggtcag ggggcgattc gttgccatcc gtacgtgctg gaagagatgg
    12361 aagcggcgaa gaacaatgac gtaaacgcgt tcgataaact gctgttcaaa catatcggtc
    12421 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
    12481 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
    12541 acctcgccct gctttctgat gtctcgatgg cggtgctggg cggcagcctg aaacgtcgtg
    12601 agcgcatctc agcccgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc
    12661 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
    12721 tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa aacttcccga
    12781 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
    12841 caccgtctga caagctggat cataaggtgg cgaagatttt acaagtgccg aatgccaccc
    12901 gttcacgtat tggtcgcggt cagtacctga ctccaagcga gcataacccg gttggtttac
    12961 tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg atctgtaaag
    13021 agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac gcgctgacga
    13081 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
    13141 gtagtattaa cgttgatgac tttgatccag aagagctggc gacgaagccg gtaaagttgc
    13201 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
    13261 tttttatcgg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
    13321 aacgccgcga acagcaccgt tttgccatca atcgaaagcg catcattcac attcagccag
    13381 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
    13441 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgagg tttgcacgcg
    13501 ctcatcacct gatacgtctc atctcccaat gtcacggttt gtgcgggagt ataagtaccg
    13561 cctttcatca cccaggcagg cagcttatgc ccttgcacca tctgattaaa cgccgctttg
    13621 gtggtttcgc cctttgcaag actgctaatg gttaaatcat cctgcgccat tgcactggtg
    13681 gcgatgacca gagcggcgac tgtcgttatt gccttaaaca tcattcctcc cgggcttatc
    13741 ctgcccatgt gattaactcc ctaaagcctg gcgataacac gataatttgc aaatcagata
    13801 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
    13861 gttagagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
    13921 cgcttttgca gcaaccactg gtgtggatgg atggtcctgc caacctgcgc cattttgatc
    13981 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg
    14041 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg aactggatga
    14101 ccgctaaagc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
    14161 ctgattcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
    14221 agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcaggc aatgagcgag
    14281 tgattgtgga atggcgcggc tggcgtattt taccgctggt gtgctacgac ttacgttttc
    14341 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
    14401 caccgcgctc tctgcactgg caggcattac tgacagcccg cgcgattgag aatcaggcgt
    14461 atgtagcggg atgtaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
    14521 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
    14581 gcattgatgc ggagctatcg atgatggcat tgcgggaata tagagaaaag tttccggcat
    14641 ggcaggatgc ggatgagttc acatttcgtt agagggtgaa tatggctacg agataagttt
    14701 cgtggctatt tttctctgga acttaaacga cgaaatcccg aatcagtaat gattcgggct
    14761 ttctgaatat tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
    14821 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
    14881 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa
    14941 atcgcccaag tcgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcaggt
    15001 tccgcccttc ttctgcacca gatcaagacg ggcttcatgt gctgcaagct cttcatctgt
    15061 cgcaaaaaca acgcgtaact tacttgcctg acgcacaatg cgctgaattg ttgtttcacc
    15121 ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
    15181 cgccagataa acttccgcaa ggatctgggc atcgagtaat gccccatgca gcgttcgttt
    15241 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa
    15301 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat
    15361 atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcaaacg ctgcgttatg
    15421 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctacct cggcaaacgt
    15481 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
    15541 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
    15601 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
    15661 accggtggtt tcagtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
    15721 ttatgtcaga cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
    15781 taaacaggta gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta
    15841 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgctggct acacccgcac
    15901 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
    15961 ttgcaaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
    16021 ccataactgg aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga
    16081 tctctggcaa cgtcttgatg ctgctctggg gcagcatcaa atcaaatggg aatgggttaa
    16141 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat
    16201 gaatcccaca ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgaca
    16261 ttgccgggtg gcaccaaccg cctggcgaat tcgcggttta ttcttgctct gtttcatcgg
    16321 atttagcgtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
    16381 aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
    16441 gctggcgtgt agcacttcaa aattcaacaa agagagccag tccagctgcc gcatcagagt
    16501 aaacatccgg ctgttatagg gcgatgtttt gcgcaatacc ggcacaagtt tgcgtaatcc
    16561 cattaaactg atgggattga agccactaat caccagccaa ccatcatcaa tcagtacccg
    16621 atcggcttca cgcaataaac gatgcggatc agtgcaccac ggcaatgtat gtgccagtag
    16681 acaaacatca acggatttat cggcaaaagg aagatgaagt gggtccgcct gcacctggac
    16741 gggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
    16801 ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg
    16861 gttgagctga cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagca
    16921 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
    16981 actgagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
    17041 gttttgaatg atgaagcagg tcgctgtctg attgtcgatc ccggggacgc agagccagta
    17101 ttaaacgcca tttccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcac
    17161 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat
    17221 ggaccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
    17281 ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc
    17341 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
    17401 cggttgtttg aagggacacc atcacaaatg tatcaatcac ttagaaagtt aagtgcgtta
    17461 cctgacgaca cattggtatg ttgtgctcat gaatatacct tatcaaatat gaagtttgct
    17521 ttgagtattc ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggag
//
LOCUS       DAPQZD010000098        17271 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPQZD010000098 DAPQZD010000000
VERSION     DAPQZD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.098"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            13..531
                     /locus_tag="RO454_003155"
     CDS             13..531
                     /locus_tag="RO454_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDY0934949.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            547..1086
                     /locus_tag="RO454_003156"
     CDS             547..1086
                     /locus_tag="RO454_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDY0934950.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1179..2756)
                     /gene="guaA"
                     /locus_tag="RO454_003157"
     CDS             complement(1179..2756)
                     /gene="guaA"
                     /locus_tag="RO454_003157"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDY0934951.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2825..4291)
                     /gene="guaB"
                     /locus_tag="RO454_003158"
     CDS             complement(2825..4291)
                     /gene="guaB"
                     /locus_tag="RO454_003158"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDY0934952.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4453..5820
                     /gene="xseA"
                     /locus_tag="RO454_003159"
     CDS             4453..5820
                     /gene="xseA"
                     /locus_tag="RO454_003159"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDY0934953.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGATLHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(5874..13955)
                     /locus_tag="RO454_003160"
     CDS             complement(5874..13955)
                     /locus_tag="RO454_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HDY0934954.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(14065..15057)
                     /locus_tag="RO454_003161"
     CDS             complement(14065..15057)
                     /locus_tag="RO454_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0934955.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            complement(15113..17263)
                     /gene="sinH"
                     /locus_tag="RO454_003162"
     CDS             complement(15113..17263)
                     /gene="sinH"
                     /locus_tag="RO454_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HDY0934956.1"
                     /translation="MLTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQTNKGKSLK
                     ERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPYGKVRSNLS
                     IGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHNFDKWLLGG
                     NIFYDYDFTRGHRRLGIGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEERPASGWDI
                     RAEAWLPAYPQLGGKIVFEHYYGDEVALFGTDNLEKDPFAVTLGVKYQPVPLIAVGTD
                     FKAGTGDNTDTTVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVERNNFIVLEY
                     KEKDPLDVTLWLKADAMNEHPECVIKDTPEEAIGLEKCKWTINALVNHHYKIVAASWQ
                     AKNNAARTLVMPVIKENTLTEGNNNHWNLVLPVWQYSSDKAEQGKLNTWRVRLALKDE
                     KGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGVVMDLDITD
                     SFGDNTDRNGNVLPQDNLNPQLFDANDKKVTLTNNPCTTETPCVFIAKQDKEKGTVTL
                     SSTLPGTFRWKAKAAPYDDSNYVDVTFLGADIGGLNAFIYRVGAAKSVNLIGNKEPLP
                     LNSRYRFVLWRDANKDGVFQLSEKLTDEEMKQYDYQWEFTGHSVNGNTGAQANTTNVD
                     IEIPATNKEAAAKFSAQVTDGVQGYGLQVNYSKT"
BASE COUNT         3911 a         4717 c         4388 g         4255 t
ORIGIN      
        1 taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca
       61 ttcactctgg caggatgcca gtcaaatgct gatgatcatg ccgccgatgt ttatcaaacc
      121 gatcaactga ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca
      181 aaagttgccg tagacaacgc ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt
      241 ggcgctgtcg ctggcggtgt tatcggccac aacgtcgggt ctggcagcaa ttccggaacg
      301 acggcaggtg cagttggcgg cggagctgta ggcgcggcag cgggttctat ggtgaatgat
      361 aaaaccttag tggaaggtgt ttctttaaca tataaggaag gcaccaaagt gtatacctcc
      421 acccaagtgg gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacgacg
      481 tataacgaaa cgcgtattca gccaaatacc aaatgtcctg aaaagagcta ataatcagga
      541 ggagtcatga agaaagtttt tctttgcgcc atcttagcct ccttaagcta tccggctatc
      601 gcctcatcat tgcaggatca actctcggct gtcgcagaag cggaacagca aggtaaaaat
      661 gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag
      721 aagcaacgtc gcgcaaacgc ccaggccgcg gctaataaaa gagcggcaac ggcagcagcg
      781 aataagaaag ctcgtcagga taaactggac gccgaagcca ctgcggacaa aaaacgcgat
      841 caaagttatg aagatgagct acgtagctta gagattcaga aacaaaaact ggcgctggcg
      901 aaagaagaag cccgcgttaa gcgcgaaaac gaatttatcg atcaggaact gaagcacaaa
      961 gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcaa acagaaatat gactgaaggc
     1021 ggtcgcgatc tgatgaaaag cgtgggtaaa gcagaagaga ataaatcaga tagctggttt
     1081 aactaaacga tgttagtaac ttcaaaccta taattcttta ggataaaaaa ccctctgtag
     1141 taacagaggg ttttgttcat tcatagtgca gggtcaaatc attcccactc aatagttgct
     1201 ggtggcttgc cgctgatgtc atacaccacg cgggaaatac cattcacttc attgataatg
     1261 cggttggaaa ctcgaccgag gaaatcgtac ggcagatgtg cccagtgtgc ggtcataaag
     1321 tcgatggttt cgacagcacg cagagagaca acccagtcat acttacgacc atcgcccatt
     1381 acgccaacgg aacgtactgg caggaagacg gtgaacgcct ggctgacttt gtcgtacagg
     1441 tccgctttac gcagttcttc aatgaagatg gcgtcagcac ggcgcagcag gtcacaatac
     1501 tctttcttca cttcccccag aacacgaacg ccaaggcctg gtcccgggaa cgggtgacgg
     1561 tacaacatgt catacggcag gcccagctcc agaccaatct tacgcacttc gtctttgaac
     1621 agctctttca gcggttcaac caggcccatc ttcatctctt tcggcagccc acccacgttg
     1681 tggtgagatt tgatgacgtg tgctttaccg gttgcagaag cagcagattc gataacgtca
     1741 gggtagatgg tgccctgcgc cagccacttc acgtcttcca gtttcagcgc ttcttcatcg
     1801 aatacttcaa cgaaaacgcg cccgatgatt ttacgttttg cttccggatc gttttcgcca
     1861 gccagcgctg acaagaagcg atcttctgcc ggtacgtgaa caatgttcag accaaagtga
     1921 tcgccaaaca tatccagaac ctgctctgct tcgttgagac gcagcaggcc gttgtcgacg
     1981 aatacgcaag tcaggttttt accgatagcg cggtgcaaca gcattgcggt tacggaggaa
     2041 tccacgccac cagagaggcc gaggatgact ttatcgtcgc ctacctgctc gcggatgcgg
     2101 gctacagcgt cgtcgataat tttcgctggc gtccacaggg cttcacactg gcagatatca
     2161 cgcacaaaac gctccagcat gcgcataccc tggcgggtgt gagtcacttc cgggtggaac
     2221 tgtacgccat agaagcgttt ttcttcgttg gccatgatgg caaacgggca gctttcggtg
     2281 ctggctacgg tgacgaagtc ggacgggata gcggtaactt tatcgccgtg gctcatccag
     2341 acgtccagca gcggtttacc atctgcggtc agagcatctt cgataccgcg aaccagtgcg
     2401 ctgtcgttta cgacttcaac ctgcgcgtag ccaaattcac gttcattaga agcctcaacg
     2461 tgaccgccca actgcatcgc catggtctgc ataccatagc aaacgccgaa taccggtacg
     2521 cctgcttcaa agacatactg cggcgcacgc gggctgtttt cttcagtagt actttccggg
     2581 ccgccggaaa gaataatgcc gcttggattg aagtcacgaa tttgtgcttc tgtcacatcc
     2641 cacgcccaca gttcgcagta aacccccagt tcacgcacgc ggcgcgcaac cagttgagta
     2701 tactgggaac cgaagtccag aatgaggatg cgatgcttat gaatgttttc cgtcattgac
     2761 gcttattccg aggcaagtga aacagataat ataaatcgcc cgacatgaag tcgggcgaag
     2821 agaatcagga gcccagacgg tagttcgggg actctttagt aatggtcacg tcgtgaacgt
     2881 ggctttcctg aatacccgca ccgctgatac gtacaaactc cgctttagta cgcagttcgt
     2941 cgatagtacc acagccggtc aggcccatac aggagcgcag gccgcccatc tgctggtgaa
     3001 tgatctcttt caggcgacct ttataggcta cgcgaccttc gataccttcc ggcaccagtt
     3061 tgtcggcagc gttgtcgctc tggaagtaac ggtcagaaga acctttggac atcgcgccca
     3121 gggaacccat accacggtaa gatttgtaag aacggccctg gtagagttcg atttcacccg
     3181 gagattcttc agtacctgcc agcatggaac ctaccatcac cgcgcttgcg ccagcggcga
     3241 tagctttagc gatatcgcct gagaaacgga taccgccgtc agcgataacc ggaatgccgg
     3301 taccttccag ggcttctact gcatcagcaa cggcggtgat ctgcggaaca ccgacaccag
     3361 tcacgatacg cgtcgtacag atagaaccag ggccgatacc cactttaacg gcactgcaac
     3421 cggcttctgc cagcgcgcgg gcacctgcag cagttgccac gttgccgccg atgatttgca
     3481 ggtcaggata tttagcgcga gtttcacgga tacgctgcag aacaccttca gagtgaccgt
     3541 gagaggagtc gatcagcaga acgtcaacgc ctgcggcaac cagcgcatca acacgctctt
     3601 cgttaccagc gcccgcgcca accgctgcac ctacacgcag acggccttgt tcgtctttac
     3661 acgcgttcgg tttacgttcc gctttctgga agtccttaac agtgatcatg ccgatcaggt
     3721 ggaattcatc atcaaccacc agcgcttttt caacgcgttt ttcgtgcatt tttgccagca
     3781 ccacttcacg ggcttcgcct tcacgcacgg ttaccagacg ctctttcggc gtcatgtaaa
     3841 cgctaaccgg ctggctcaag tcggtcacaa aacgcacgtc acgaccggtg ataatgccga
     3901 ccagttcgtt ttcttcggta acaaccgggt agccagcaaa gccgttacgc tcggtcagtt
     3961 ctttcacttc acgcagcgtc gtagttggca gtacggtctg cggatcggtt accacgccag
     4021 attcgtgttt tttcacacgg cgaacttctt ctgcctggcg ttcaatggac atgtttttgt
     4081 ggataaagcc gataccgcct tcctgagcca gagcaatagc caggcgcgct tccgttacgg
     4141 tatccattgc tgcggaaagc atagggatat tcagacgaat agttttcgtc agctgagtgc
     4201 tgaggtcagc agtatttggc agaacggtag aatgagcagg aacgaggaga acgtcgtcaa
     4261 acgtcagagc ttctttagcg atacgtagca tgggcaatat ctcgaccaga gtggttaata
     4321 aatattgccg cggcattata cagagcgtaa ccgattgcat ctaccccttt ttgcaaaaaa
     4381 tgcttgctat ccccggaggg cgggttacta tcgactgaat aacctgctga tttagaattt
     4441 gatctcgctc acatgttacc ttctcaatcc cctgcaattt ttaccgttag ccgcctgaat
     4501 caaacggttc gtctgctgct tgagcatgag atggggcagg tgtggatcag cggcgaaatc
     4561 tctaatttta cacaaccagc ctccggtcac tggtacttta cgcttaaaga cgataccgcg
     4621 caggtgcgct gtgcgatgtt ccgcaacagc aaccgccggg tcaccttcca cccacagcac
     4681 gggcaacagg ttttagttcg cgccaatatt acactctacg agccgcgcgg cgattatcaa
     4741 attatcgtcg aaagtatgca gccggccggt gagggattgc tgcaacagaa gtacgaacag
     4801 cttaaagcga agttgcaggc tgaaggtttg ttcgatctgc aatacaaaaa atcactcccc
     4861 tcccctgcgc attgcgttgg tgtgatcacc tcaaaaaccg gtgctacact acatgatatt
     4921 ttgcatgtgt taaaacgtcg cgatccttct ctgccggtga tcatctaccc caccgccgta
     4981 cagggggatg atgcgccggg gcaaatcgtt cgtgccattg aactggcgaa taagcgcaac
     5041 gagtgtgacg tgttgatcgt tggccgtggc ggcggttcgc tggaagattt atggagcttt
     5101 aacgacgaac gcgtggcgcg ggcgattttt gccagtcgca ttccgatagt cagcgccgtc
     5161 gggcatgaaa ccgatgtgac gattgccgac tttgttgctg atctgcgtgc gccaacgccg
     5221 tcagccgccg ctgaagtggt gagccgaaat cagcaagaat tgctacgtca ggtgcaatct
     5281 gcccgtcaac ggctggaaat ggcgatggac tattatctcg ccaatcgcac gcggcgcttt
     5341 acgcagatcc atcaccgctt gcagcaacag catccgcagc tccggctggc acgccagcaa
     5401 actatgctcg aacgtctgca aaagcggatg agctttgcgc tggagaatca gcttaaacgc
     5461 gcgggtcagc aacagcagcg attaacacgg caactcgtcc accaaaatcc gcagtcgcgt
     5521 attcatcgcg cgcaaacgcg cattcagcaa ctggaatatc gtttagcaga aaccctgcgc
     5581 gcacagctta gcgccacgcg cgaacggttt ggtaacgcgg taacacacct cgaagcggtt
     5641 agcccgctgt caacgctggc gcgcggttat agcgtcacca gcgccgccga tggcgcagtg
     5701 ttgaagcagg tgaagcaggt gaaagtgggc gatacgttga ccacgcgact gggagatggc
     5761 gtggtcgtca gtgaagtgag tgcggtgacg aaaacccgca agccgcgtaa aaaaacctga
     5821 tagcccgttg cgtatcaggc tttcggaacc tcatattgat agggtgggca aggttattgg
     5881 aaacaggttg caaaccccgg cgttttcgca ggctgaatgc cacagttgag actgccagag
     5941 ccttcctcaa caccacaata gaacgatcca gccttatcag tagataaata cgggaagctt
     6001 atcgatgacg gccatcccaa tgtgctaaag ccggagtgta atgtttgtaa ttcggcactg
     6061 ctcggcacgt tattcatact accgcactgc cgtgcgacat cccatttagt ggaatctata
     6121 acatgtgcca gcgcccaggt ttcattattc acattgggcg ttgctgctgc ggtaacccct
     6181 gacggcaact ccgattttaa tagcgggcga tgcagcatct tgccacttac cgtggcggta
     6241 tccgccatat gaccccagaa atacgccgtg gcgacattag gactggtcgg cacggtgaaa
     6301 atcgtgtcca gcgtcgccgt tattgtggcg ttatccgtca gcgtcaccgt caacggcgtt
     6361 ttatatcctg gcgtattatc ctgaccaacg gtaaaacttg ctgtaccgtc tgctccagta
     6421 actccatcga ttgtattccc gctatcagcc aacgtgactg ccgcaccgga tgagggttgt
     6481 acagtcaaat cgtccattgt atccacatcg ccatacaacg tcgccccgct attacgcggc
     6541 gtagcgtcac cacgtttgag gataaacgaa atattcggca ctggtttccc gacactgtct
     6601 ttcacggtaa cgacaacggg aacggcgtcg ccttttttca ccttcgccgc ctgcgtactg
     6661 ctatccagtg ccgtcgatgt caacgtgacg ctggagacag tcgcacgcgg gttaaccagg
     6721 cacccttgat acgcggtatt caccgtcccc cacgctgaac cattattcag accgactacc
     6781 tgcggattac tgcctcccag ccagttatcc agcgcccacc atgtatactg cgtagaccaa
     6841 ccgatttgtg tcgtcagcgt gttccccgga tagcgagcat agagcgattc taaatcatca
     6901 ctcaatggcc gacgtgccgc ttcacaaggg ctgagattgc tgtcagccct ctgcgttgcg
     6961 ttaaacacgg gccagacttc attgttacta ttgaccttgc taacccctga tgtcgacggc
     7021 agctctttcc acaatttagg tctttcaaag accacaccgt tttctgccgt taccgtatcc
     7081 ggcatatgac cccagtaggc cgccattgcg gaatcagggc tggtgacaac ggtgaagatc
     7141 gcatcaagga cagtggtggt ttccgtatca cgtgccagga tggcggtaaa cggtgttgcc
     7201 aggccgacag tatcaggctg ttcaacattg aatatcgccg taccatcgct gccggtaaaa
     7261 ccaagccatt gcgtgccgtg tgaggcgaga gtgaatgttg ccgacacggg tgccagttcc
     7321 gtcaatacca tcacatttgc tgccgtgttt acaatggttc ctgagcggct catcgcatta
     7381 tcacgcctga gcgttatcgc ttcgcccgcc agcgggttgc catcacgatc tttgaccata
     7441 accacaattg gcaatgggtt atcttttttc actgacgcgc taggtcggcc gccattgctg
     7501 agggttctgg cgtcatccag tgcggatgac gtcaatgtta cgctaacgcc tgccggatgc
     7561 ggctgcgcca gacagagcat cagtgcgttg gttgcagaat tcttcgcgat agctcctgtt
     7621 ctcaggctga cagtctgata agaccacgtt gttgtaccca gtatggccat atcataagcc
     7681 caccagttac ccgcattcat cggtaaccca aggtcagtca ttattgcgcc gttcggatga
     7741 tcactataca aatccaacag ttccttactg cgcggttgat tctcgagtgg gcaatctgta
     7801 gatgttaaat tttgcaagtt gctcatatac gaccaaactt cgccgttaga actaatcgat
     7861 aagcctgagc tggtttcggt tttcagcagg ggtcgtttga aggtgacgcc cgccgaactg
     7921 gtaaaggtgt ccggcatatg cccccagaat ttagcactcg gtgaatctgg gctggtaatc
     7981 accgtgtaga taacatccag cgtggcgact ttcgttgtat cacgcgccag cgtcacggtt
     8041 aacggcgtcg ccaacccggt agtagagtac tgcccaacct caaaggtggc tgtgccatca
     8101 ctgcccgtcg ttgcgagagt ttttgctcca cttgctgtca acacgctatc cctactcacc
     8161 ggaaccaatg ccgttacggt aagatcgtct gcactcgtgt taacggttgc tttcgcgcga
     8221 tccagcgtcg tttcacgcga aagggtaaag tcggcatatg gcatcgggtt cccggcgcta
     8281 tcacgcacgg tcaccgtcaa cggaatcagc tcacctttct tcgctacagc gctcggtcgt
     8341 ccatcattgc tgagcgattt tgctgcatcc tgcgccgttg aggtaagctc tattgtgact
     8401 gcttccgtat gaggctctac caaacacatc actatcgccg tatagctgga cgatgcctga
     8461 atcaactgac catttcccgg aacaaacgcc tggttactcc agctaccgcc cgctaccgcc
     8521 atcatgtcat aatcccacca gttccctgca gcagtcggca agccgagcac cgttgccagt
     8581 tgaccattgg gataatcgac atacagcccc tgcatatcag tcgtcagcgg ttgatacgcc
     8641 tcatcacacc cagccttgct gacgtccgct ttctcagtcg cggtcaggag cgaccagact
     8701 tcattattgg ccgttttcgc cgtaccgcca gagagttcgg ctttgaggaa aggccgtttg
     8761 aaggtgacgc cttcgctgct ggtaaaggtt tccgccatat ggccccaatt ggtcgccagc
     8821 ggcgtatccg ggctggtggt gacggtaaag atcaccgaca aggtgtccga cgacacgcca
     8881 gtatcgttta atagagcata aaagtcggtt cttaacccag tggaagcatc ctgtcggagc
     8941 gtcatcgtag tggttccatc tggccctgtt acaccataca ggatggtcgt tgcactggta
     9001 tccaccgtcg cacccgccgc agtcgtgact gacactttct gcgatgacgt cacattctga
     9061 cgattgatcg agttcgcgtg tttcagggca aacggcgtat tgccgactaa attcccctgc
     9121 gcatctttag tcgaaatgcg gatcgacacc tcatccccct tcttcgcttt gacggcgcta
     9181 agtgtgctgt caatgctgac aaactgcccg gggtctgagg cttccagcgt aatctggctg
     9241 gcagtcatca acggcgttgt acggcaggtt tgtaacgcca gcacggtgga tacgatttgc
     9301 gaaactgcgc cagaatttag ttgcaccaca ttgtagtaac ggctccctgt ttgtgtatta
     9361 ctcggggtat tggtgagata actcatattt accggccatc cctgtaccgt attcatcgca
     9421 ttacctctat tggcgtcata caaagacttc agaccatcca gcgttggcat ataatctgta
     9481 ccacacccat tcatgctggt gtcagccgac tgagtcgagg tgaatcttgc ccaactttca
     9541 ccgttctcaa acgtgctata tcgtgcatag ttgttggtaa gctcgccaaa cagcaacggt
     9601 cgcttataaa ccgaaccatc ggaggccgtc accgtttcgg ccatatgtcc ccaaaaattc
     9661 gcttttggcg aatccgggct ggtcacgacg gtaaagacca ccggtagcat cgccttgacg
     9721 ctgctggacg tatccagttt tgccgtgaga tcggttctta acccggtact gttatcctgt
     9781 tttagggtaa atgtcgttga cccgtcggca ccggtcacgc cgtaaatggt ttcactcgtt
     9841 gactggaaat tatttcgcgt atttccccac gcatccgtca ccgtcagcgc gcccacactc
     9901 atcgtcgccg atgaattagc ccgacttaca ctgttggctc gcgtcaggat aaatgccgta
     9961 ttgcccacaa ggttgccttg cgcatctttg gtgatgacgc gcaccacgga ttcttcgctt
    10021 tttttcgctt ttaccacgtt ataggtggta tccagcgtca ccggatctgc cgcttccagc
    10081 aggatttggc tcaccgcggc tatcggcgcg gtctggcagg tcagcagctc cagcgtcgtt
    10141 gatgacactg ccgcactcgt gccattgctg agatctaccg ccttgtagtt acgctgctcc
    10201 atgctgccgt tgtcggaagt gttggataaa tagcgcgttg ccaccggcca gccctgcacg
    10261 gttttcatgg tgttgcccgc attattagca aacagtgacg tcagaccggc ctgagtcgga
    10321 atatagttgg ttccacagcc gctgtacgtc gtggaaaaac ttgcataatt aatgttaaac
    10381 aacgcccagt tttcattgtc ttcagacgtt gatgttcgcc cagtctgata ggccagttcc
    10441 ttcaacaaca gcgggcgctt aaataccgta ccgtccgcag cagtcaccgt ttccggcata
    10501 tgtccccaca tttttgccag gcggctatcc gggctggtca ccaccgtaaa gattgtgtcg
    10561 atgctggatt ttttcgtcgc atcagagtaa agcattgccg tcagcgccgt tttggtgccg
    10621 tgagtatccg ggcgggtaat attgagaatt ttgctgccgt cattgtcggt catggcggta
    10681 tacaccgttg cagtgtcgtt caacgtggtt tcatctgcca gcccgccatt gactaccacc
    10741 ggcgcaacca gcgcatcgcc gctgcccgcc acatgttttt cgccgctacg ggtatagcca
    10801 tcaccgcgat tgagggtaaa tggcatatcc gctaacgcgt tgccctgtgc gtcttttacc
    10861 gtcactttaa cctgtagcgt ctcgcctttt ttcagcgttg ccgcgctatt cccggtattc
    10921 cattgcgctt tatcaaccac ctccagcgta atgctgctgg caggtttgtt cgccgtcttc
    10981 aggcaactga cgtaaaccgg cgtatcgccg ccaatagttt gtgcgccatc gttcagcgct
    11041 atcgtgaaaa agtgtggagt tttatctgcc ggtgagttgc tccagtaagg ctgacgctgt
    11101 gttggccaac cgtgcgccgt ttgcaccgcg ttaccactgt tggcgttata cagcgcactt
    11161 agctgggaac gacgtggcaa catattggtg tcgcagccac ctttactggt actggacgta
    11221 ttggcctggc tcacgcgcgc ccagatttcg ttattttcgg tcagggttcc gtccgggctg
    11281 gcgacttccg acgccagttt aggccgggta aaggttgatg aatcaacggt gatcgtttca
    11341 tccatatggc cccacatctg cgcgccggta acgtccgggc tggttggggt agtaaaaata
    11401 acgctgtagt taacggtatt cggcaggtag gaatttaccg gcgttacgct cagcaccgtt
    11461 tttagcccga cgccctgtgg ttgcttaatc tcgaccgtcg ccacgccccg ggcatcggtt
    11521 attcccgcat aaaccttcga tgactgtgat gtgccgtatt gcgtgttatc catcgtgatt
    11581 gcaccactgc tcgggtcatc aaacccgctc acctgccccg ctcggttata ccccatacct
    11641 ttggtaatgg tgaatgcagt aaagggtaca gggatattat tgagcgagtt aatcgtacgc
    11701 acggtcatca caatggtgtc acccactttg gcttgcgctt gggtgccaac agacacatca
    11761 tgatcggtgc tgacttcaac attcgctacc atctcattac ccgaacagga gaggtaattc
    11821 ggcttaaagc cggaatagct gtcgacgctc ccgttcccca gatccaccga tgaatgcgtg
    11881 gcttgctcca ccgcgcttaa atagccctgt tgtgcagtgg gccagccata ttcagtacca
    11941 atcgcgttac cgggatgggc tgaaaacagg ctttccagtg agctctggcg cggtatatga
    12001 ccgacgccgc attccgcctg catactggtg ttttgatcgt agagcgccca gtcttcgtta
    12061 ttttcccgca ctgttcccaa ttcatgctcg gtttcatccg ccagcaacgg gcgtttatac
    12121 aaactgccgg actcaataat gccgcgcata tggccccaca tccgcgcttt gtcgctgtcc
    12181 gggctggtga ttacggtaaa aatcacatct ttggcatctg tggcgttaaa atcgctgcgc
    12241 attcttgccg tgatatgggt tttcaccccc gcgccgccgg gctggctcag ggtgagcgaa
    12301 gcctggccgt tggcgtcggt catgccttca taggtatgtg cgtcgacctg gcgaaaatta
    12361 ctgccgccag caatctgtac cggatgggct tcccaggcag tgtcggtttg attcttacga
    12421 ttgacgccat catccagatg tagcgagaag tagtaatacg ccagcggttg atcgttgttt
    12481 ttcgtgtcgg tgatggccag acgcatcgtc gcatcctcgc ccaccttcgc tttcatcgcc
    12541 tgcgccgtgt catcatagtt ttctggcgtg aggacgattt tcggctcgac gtcaggcgcg
    12601 ggcttatcca cgcagctcac gaggaacgtg gtgctgtccg gcttctgcgt ttcgccacgg
    12661 ttagagacat ctgccgcatg gtgctgcccg gtaagccccg ccagcgatga ccagtaataa
    12721 tcgccctgca tcggccagcc gagcacagtc tgtaccgtgg agggaatcac cgtcgccagt
    12781 gcgccgaagt ggcgcatgcc cggcagcacg gtgcaatggc tatctgcgcc gctccaggta
    12841 aaggtggacc aggtttcatt gtgatcgacg accgtggcat tttcgttact gacttccgcc
    12901 gccagttttg gtctactgaa cgtgtaacca tgggcttcca ccgtctccgc catatggccc
    12961 cacatcgtgg cctgcgccac gtccgggctg gtgagcacgg taaagatcac cgccgcctca
    13021 ctggtttgtg caatgccgga aatgccgact accagcggcg ttttaacacc ggggccgtta
    13081 ggctgggtca ccgtgatggt tgccgtaccg ttagcatccg atgtgccacg atactcggtt
    13141 accgtcgtcg ttaactcggt gctatccagc accaccggcg cggtattatt taccgcgccc
    13201 tgacggtttt gggcatcttt acgcttgata ataaatggaa tattacccgc gacgttaccc
    13261 tgacaatctt tggtggtgac ggtcagcgtg atagtgccgc caacggtggt ttttgccgcg
    13321 ccgagcgttt cgttataacc actggcgctc accgaaatta aatcgccctg cgcgggcgcg
    13381 atattcagcg tcactttctg gtcagccagc accaccgtcc cctcataggg ataaccggtc
    13441 gaggtcagca gataaagcag tggaatggtc tgctgcgcat caatctgtgg ccaggccacc
    13501 agcgaacgcc cggccagacc ttgcgcgcaa aagctgcggt tggcgatggg ctgtggattg
    13561 agcggcgtat ccggcgtggc ggcatccgcc cacaccagac tgacggtcgg cggcgtctgg
    13621 ttttcccagc gcagcgcagg tggtgtagaa aactggtcgc cttcactgtc ctgaggatta
    13681 gcaactatca ggttagcagc gtcatcgtca acgctaaatt cattcggttt agcagaaaac
    13741 gccacatcat gagatgcagc aggatcaagc tgaacgcttc cctgataaac gggtacgctg
    13801 gcgctatcgg caataggaac tgtaccgtta aactcgcctg tagaaccagg atatgtctgc
    13861 cattccacct ctgcccgtgc aaagtcagac agcataaagg ctgccaacat gcccagaagc
    13921 acaatgattt ttcctggttt cagacatacc gacatgtaga aaactccagc atgataaaca
    13981 aaacgtcaac ccgttttgct taacgcgtcg ctaagcaaaa caggcggaaa acagcattcc
    14041 catacgggaa tgctgttgtg cgcattagtg gtgtttgtaa atgatagaca gcgtatagcc
    14101 ctgaacgccg tcgccgtttg ccgggattgc aaggccggtc ttaccattac ggtcactacc
    14161 gttaaggctg gttcttgctt gttcgttagt ttgcggaata acaatgtcat tgttatcgaa
    14221 gctggcgttg gcaataccgc ccgccgccgc aagttgttta ctgttaccgt taaagaccca
    14281 cgcaaagtcg tagttagcgg taacgtccac atccgtaccc gcgtcgtatt taccgttctg
    14341 gttttcatcc gagtacagtt tggccacata cgttttgtta agcgcaatgg ctgtattacc
    14401 accgataagg ttggtactgg tgtctttctc gaagatggca actttcaggt tctggttaac
    14461 aaccggacca ggatcgacat tatccgtatc gctgccgcca ccgctggccg tagaaatatc
    14521 cagcagagtc agcgaagttc cctggaaggg attaccggtc tgagtgatag gctggatttc
    14581 aacaccgata tagcgtccac gatcgccatc ggtgatttta taactggttt ttcctgccgc
    14641 cgcagtcaac tccgttttac cgccacccgc attatcactg tagctgtacc atttgatggt
    14701 cgccgtcgtt ttcgcatcaa tgcctgcgga accatcatcg atatcccctt cggtatcgcc
    14761 gatatcccaa cccagcgtga tggtatcccc tgagtaaagg cgtttatcag actcgctacc
    14821 tgcaggacgg atacctttac tatcagatgt cactttgatg tgatccgcat cttccgagtt
    14881 aatcgtagca ttcccccctt cgcgatagag ccagggtatg gtcccctcga attcagctgt
    14941 cgttccactg gcgacccagg cagcatcttc cgccatagcg actggtacgg cacagtatcc
    15001 agccagcatc agtgccagcg cgacttttgt caggccacgt ttcatatgag gtttcataaa
    15061 cactctcctt ggttaacatt cagcggtggc ccgatcagcc atggcgctgt tcctatgtct
    15121 tgctgtaatt gacctgcaaa ccgtaccctt gcacaccatc ggttacctgg gcactaaact
    15181 tcgctgccgc ttccttgttc gttgccggga tctcgatatc gacgttagtg gtattggctt
    15241 gcgcgccagt attgccattt acgctatgcc ccgtgaattc ccattggtag tcatactgtt
    15301 tcatctcttc gtcagtgagc ttttcagata gctggaacac accgtctttg ttagcgtcgc
    15361 gccacaacac aaagcgatat cggctattga ggggcaacgg ttctttattg ccgataagat
    15421 tcacggattt agccgcacca acgcgataga taaaggcatt cagcccgccg atgtcagccc
    15481 ccaggaacgt tacgtcaaca tagttactgt catcataggg ggcagctttc gccttccaac
    15541 ggaaggtgcc aggcaacgta ctggagagcg taacggtccc cttttcttta tcttgtttgg
    15601 cgataaagac acagggagtc tcggtagtgc aggggttatt cgtcagcgtc acttttttat
    15661 cgttggcatc aaacagctgc gggttgagat tatcctgcgg caagacatta ccgttgcggt
    15721 cagtgttgtc gccaaagcta tcagtaatat cgagatccat caccacacca tctacaccat
    15781 cagcctgtgc actggcttcg tggctgtggt tgttctcttc cggcacgttc gcgatgttat
    15841 taacaatcag ctcaatttta cggtcctgct gaacggtgat ttccaccacg cctgagttct
    15901 gtcggttgcc cttttcatct ttcagcgcca ggcgaacccg ccaggtattg agttttcctt
    15961 gttcggcttt gtcagagctg tactgccaga cgggcagcac caggttccag tggttattgt
    16021 tgccctctgt cagggtattc tctttgataa ccggcatcac cagcgtgcgg gcggcattgt
    16081 ttttcgcctg ccatgacgca gcgacaattt tgtagtggtg gttgacaagc gcgttaatgg
    16141 tccatttaca tttttccagc ccgatagctt cttctggcgt atctttaatc acgcactcgg
    16201 gatgttcatt catcgcatca gctttcagcc acaaagtgac atcgagggga tctttctctt
    16261 tatattccag aacaataaag ttgttacgtt caacaaagtc gtgacggctg cccatgagtg
    16321 agtgagccgc gctcactttg tcaggatcaa gctggtcttt gagcgggacg ccaaactggt
    16381 agttaagcgt tgcgttgacc gtggtatcgg tgttatcacc ggttcctgct ttgaagtccg
    16441 ttcctacagc aatgagcgga acaggctggt atttcacgcc taacgtgaca gcgaagggat
    16501 ctttctctag attgtcggta ccaaatagcg caacttcatc cccgtaataa tgttcgaaaa
    16561 cgattttacc gccaagctgc ggataagcgg gaagccaggc ttcggcacga atatcccaac
    16621 cgctcgcagg gcgttcttca taaaagtcga aatcttcaga gtctttccag tcagaaagcg
    16681 gatgataata gttaccagaa aattttaaat aatcagtcca cgcttcagcg cctataccta
    16741 aacggcgatg tccacgggta aaatcataat cataaaatat atttccaccc aataaccatt
    16801 tatcaaaatt atgtcttaca ccaagcccta tatttccgat cgtacgatct tcttttcgtt
    16861 gcgcagaaaa ttggctgaaa taaacagtgg tttgattatc gtaccaggga acaaaataat
    16921 cgatggaact gccatccaga tcgccgccct gaccaatgga gaggttcgaa cggaccttac
    16981 cgtagggaga gagtgtgtct tcaatataag attgtgcggt tgaggtgatt tgactttgca
    17041 gatagcttct ggcctgagat tccagcgcct caggggtcaa gttttcaaac ccttgcgtgg
    17101 cggagttgat gacgtaatcg gctccgcgct ctttcagcga tttacccttg ttggtttgtt
    17161 catcctgttg tgctgcctgg ctacctaaat caggtaacga ttcgttttgc ttaacattta
    17221 tctctggtgc cgcgaaagca gcaccagaga taaatgttag cataatacag c
//
LOCUS       DAPQZD010000099        16914 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPQZD010000099 DAPQZD010000000
VERSION     DAPQZD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.099"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            543..695
                     /gene="mokC"
                     /locus_tag="RO454_003163"
     CDS             543..695
                     /gene="mokC"
                     /locus_tag="RO454_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDY0934957.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(799..1929)
                     /gene="dnaJ"
                     /locus_tag="RO454_003164"
     CDS             complement(799..1929)
                     /gene="dnaJ"
                     /locus_tag="RO454_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDY0934958.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(2018..3934)
                     /gene="dnaK"
                     /locus_tag="RO454_003165"
     CDS             complement(2018..3934)
                     /gene="dnaK"
                     /locus_tag="RO454_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDY0934959.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4305..4709
                     /locus_tag="RO454_003166"
     CDS             4305..4709
                     /locus_tag="RO454_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDY0934960.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4735..5448
                     /gene="msyB"
                     /locus_tag="RO454_003167"
     CDS             4735..5448
                     /gene="msyB"
                     /locus_tag="RO454_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDY0934961.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5597..6163
                     /gene="satP"
                     /locus_tag="RO454_003168"
     CDS             5597..6163
                     /gene="satP"
                     /locus_tag="RO454_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDY0934962.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6198..6785)
                     /gene="mog"
                     /locus_tag="RO454_003169"
     CDS             complement(6198..6785)
                     /gene="mog"
                     /locus_tag="RO454_003169"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDY0934963.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6900..7853)
                     /gene="tal"
                     /locus_tag="RO454_003170"
     CDS             complement(6900..7853)
                     /gene="tal"
                     /locus_tag="RO454_003170"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDY0934964.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8132..9562
                     /locus_tag="RO454_003171"
     CDS             8132..9562
                     /locus_tag="RO454_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDY0934965.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9632..10408
                     /gene="yaaA"
                     /locus_tag="RO454_003172"
     CDS             9632..10408
                     /gene="yaaA"
                     /locus_tag="RO454_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDY0934966.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10448..10744)
                     /locus_tag="RO454_003173"
     CDS             complement(10448..10744)
                     /locus_tag="RO454_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDY0934967.1"
                     /translation="MKKMQSIVLALSLVLFAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10958..12244)
                     /gene="thrC"
                     /locus_tag="RO454_003174"
     CDS             complement(10958..12244)
                     /gene="thrC"
                     /locus_tag="RO454_003174"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDY0934968.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHGGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12245..13177)
                     /gene="thrB"
                     /locus_tag="RO454_003175"
     CDS             complement(12245..13177)
                     /gene="thrB"
                     /locus_tag="RO454_003175"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDY0934969.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(13179..15641)
                     /gene="thrA"
                     /locus_tag="RO454_003176"
     CDS             complement(13179..15641)
                     /gene="thrA"
                     /locus_tag="RO454_003176"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDY0934970.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15668..15787)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15723..15788)
                     /gene="thrL"
                     /locus_tag="RO454_003177"
     CDS             complement(15723..15788)
                     /gene="thrL"
                     /locus_tag="RO454_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDY0934971.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16002..16688)
                     /locus_tag="RO454_003178"
     CDS             complement(16002..16688)
                     /locus_tag="RO454_003178"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDY0934972.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3994 a         4453 c         4134 g         4333 t
ORIGIN      
        1 agaatgacca ggccagtata tttaaagatg actatcgaag cgtgatgaag atcgcagatt
       61 acttttgcga acgggcggag ggatatcgtg tttcagaaaa tgtgtctgtt ttaaatatca
      121 ccctttgatt tataggcatt tttcatgatg aacttagcat tggctgctga acgtatttct
      181 ttgtctctct atagtattac actaaccttt aataattaat ttcctttagc cattcacttt
      241 tgatagagga aaacaccttt tttgtgaatc cctggatata tttaagttaa ataaattgat
      301 gtattgaaat attattccgc aaaaatgcga ggcgtcttca gaaaaagaat tgataaaaca
      361 gaagtgaatt aaaagcgcag tataatagcg gcgggtgctt gaggctgtct gtctcaggca
      421 ttggctgaac ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggccc
      481 cctatatgca gaacacatgt agagtgcctc ttactgaccg aaaggtcaag gagaagagag
      541 caatgaagca gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag
      601 tggcggcgct ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg
      661 aggttgctgt tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg
      721 ccactttaca ggtgctcgca tatcttcaac gcaccctttt ttttacctgg cctgcccacg
      781 ggcaggcttt tggggaggtt agcgagtcag gtcgtcgaaa aacttcttca caccatcaaa
      841 gaagctcttt gagcgcgggc tgttgtgctc gccggttggg ccaccaaagc tttcttgcag
      901 ctcttgcagc agctgtttct gcttctcgtt caggcctacc ggtgtttcga cgacaacgcg
      961 gcacagcaaa tcaccctgtg cgccgccgcg gacagacttg acgcctttac cacgcatacg
     1021 gaacagctta ccggtctggg tttcgccagg aactttcagt ttaacgcgac catcaagggt
     1081 cggcacttcg atttcgccgc ccagcgccgc catagcgaag ttgatcggga cttcgcaata
     1141 caggttgttg ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag
     1201 gtcgcctgcc ggtgcgccgt gttcgcccgc ttcaccttcg cccgcaagac ggatgcggtc
     1261 tccagtgtcc acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc
     1321 atgaccatga catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg
     1381 acaggtctgc tgcacggcaa agaaaccctg gcgcatctgc acctggccag aaccatgaca
     1441 ggtcggacag gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca
     1501 ctcttccaga gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt
     1561 gagctccatg ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc
     1621 gccaaaaata tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa
     1681 accgccgccg cccatgccgc cttgctcaaa cgcagcatga ccatactgat cgtatgccgc
     1741 acgtttttgc gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc
     1801 ggcctctttg tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc
     1861 ctttttgatt tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg
     1921 cttagccatc ttttttgatt tgcccctaga tgaatgcacg ggcggagagg aaattcccct
     1981 tcgcccgtgt cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt
     2041 caaattcagc gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg
     2101 cagtctgctg ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg
     2161 ccagttcctg cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca
     2221 gtgcagtcag cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt
     2281 cttcaacctg cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca
     2341 gctcttcaaa cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga
     2401 tttcatcttc gttcagacca gaagaagcct tgatagtgat cttctgctct ttaccgctgt
     2461 ttttgtcttt cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga
     2521 tctgcggcat gccgcgcggt gccgggttga taccatccag gttgaactga cccagagatt
     2581 tgttatcagc cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt
     2641 tgtcttcagc ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga
     2701 tcagcgtggt catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca
     2761 gcagcagtac gtcttttacg tcaccagtca gaacaccacc ctgaacagca gcaccgatgg
     2821 ctacagcttc gtccgggtta acatctttac gcggctcttt accaaagaat tcagcaactt
     2881 tcttctgaac cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag
     2941 atacggacag gccagcgtcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca
     3001 gatcttcaac caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac
     3061 cggtcgcgtc tgcagtgatg tacggcaggt taacgtcggt ctgctgagcg gaagacagtt
     3121 cgattttcgc tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca
     3181 ggtcaatgcc ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga
     3241 agtcttcacc acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc
     3301 cgtcaacttc gtcgatttcg ataatggaaa tatcgaaagt accaccaccc aggtcataaa
     3361 ccgcgatagt acggttgcca gtacctttgt ccagaccgta agccagcgca gctgcggtcg
     3421 gttcgttgat gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct
     3481 gacgctgagc atcgttaaag tatgccggta cggtaataac agcttcagtt accggttcac
     3541 ccaggtaatc ttcagcggtt ttcttcattt ttttcagcac ttcagcagag atctgcggcg
     3601 gtgccatttt ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt
     3661 tgaacggcat gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca
     3721 ggcgtttaat cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct
     3781 gaccaaccag agtttcacca tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc
     3841 cttcggcgtt ctccagtaca cgaggagtgg tgccatccat aatcgctaca caagagttgg
     3901 tagtacccag gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt
     3961 catcatgtgg ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc
     4021 atttttttgc agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat
     4081 caagggggag atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga
     4141 caagacgtga aactgacagc aattttattc atataaagaa tgaatcgcag caaggcattc
     4201 atcaaaaaat tgtaataaaa agaaaatatt acgtgcctga aacttctctt tatcagcggt
     4261 aaactagttt catcccctta cccctcccca ccaagagaat ccttatgaaa tccgttatta
     4321 cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc
     4381 ataaaatcct cggcgtcatt gcaatgccgc gaaacgaaac caacgatctg gcgctgaaac
     4441 ttcctgtttg tcgcattgtt aaacgcatac aactctcagc cgaccatggc gatttgcagt
     4501 taagcggtgc atcgatttat ttcaaagcca cccgaagcgc cagtcagacc ctgaatattc
     4561 cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca
     4621 acaaacgctg cgtctcaaaa atcacctttt ccggtcatac agtgaactca tcggatatgg
     4681 ccacgctgaa aattatcggc gacgactaac gccagaatat agaaaccaca aaaaatgaat
     4741 gttaattacc tgaatgattc agatctggat tttctccagc attgtagtga ggaacagttg
     4801 gcaaatttcg cccggctgct cacccataat gaaaaaggca aaactcgcct ctccagcgta
     4861 ctgatgcgca acgaactgtt taaatcgatg gaaggacatc ccgagcgaca ccgccgcaac
     4921 tggcagctga ttgccggaga attacagcat tttggtggcg atagtatcgc caacaaactg
     4981 cgcggacacg gtaaattgta tcgcgccatt ttgctcgatg tttcaaagcg attgaagctg
     5041 aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg
     5101 cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat
     5161 gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca
     5221 aaaggtgtgt cgcatttgct ttccagccaa ttgacccgca ttttacgcac ccacgcagca
     5281 atgagcgtgc ttgggcatgg tttgctgcgc ggcgcgggtc tgggaggccc tgtgggcgca
     5341 gcattaaatg gggttaaagc ggtcagcggc agtgcctatc gcgtgacaat tccagccgta
     5401 ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc aggtctgata ccggaaaccg
     5461 gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta
     5521 tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga
     5581 tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat
     5641 gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt ttgctctgga
     5701 cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgccggtct
     5761 gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt
     5821 ctggctgacg ctggttgcaa ttctgctgat gccgaaactg gggctgaccg atgcgccaaa
     5881 tgcacagttc cttggtgtct acctgggtct gtggggcata tttacgctgt ttatgttctt
     5941 cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga ccgtgctgtt
     6001 tgccctgctg gcgatcggta acattgccgg taacgccgcg atcatccact ttgccggctg
     6061 gattggtctt atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tgctgaacga
     6121 gcagtttggt cgcaccgttc tgccgattgg tgaatcccac taattgtttt gagatccccc
     6181 cgctcggggg gattttttta ttcgctaact tcgcgtcttg cactcttcgg tctgaatgct
     6241 gcaaccactt ccggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt
     6301 acgctggcaa aaataccgtg caccacaacg ttaccctcag cgtccttcac accttccagc
     6361 gtctctttaa tagacttcgg ttgaccgggt aagttaagga tcagcgcctg tttgcgaatc
     6421 acccccacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc
     6481 tgttcaccaa agcctggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg
     6541 tcacgacgcg ccgggccagt tccgcccgtg gtaagcacca gatggcaact catttcatcc
     6601 accagctcac acaacgtttg ctcgatgatc gcctgctcat cgggaattaa gcgggtttcc
     6661 agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgcagg gatgccttta
     6721 tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta
     6781 ttcatcaaga tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga
     6841 gtgacagaca ggtttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat
     6901 tacagcagat cgccgatcat tttttccagt ttttcctggt caacagcaaa cttacggata
     6961 ccttccgcca gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac
     7021 tcagtgatac gcgccggacg cgctttcact tcaccagtgt aagacagttt acgttcgata
     7081 gccccttcgc tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcgcag
     7141 cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca
     7201 taaccgtgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc tggatcttct
     7261 gccggagcgt actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca
     7321 aacggcgaga tcaggaacac gcccgcttcc gcacaagcac gagcctgagc gaaggagaac
     7381 agcagggtca ggttacagtt gatgccttct ttttccagct gttccgcagc acggataccc
     7441 tgccaggtag aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag
     7501 agtttgatca ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga aagacgcgca
     7561 tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttacg
     7621 gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc
     7681 caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg
     7741 agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca
     7801 gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta
     7861 tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg
     7921 caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct
     7981 gacactgcgt tgatatcgcc cgccattttt atacaaaacc tcatgtatgc tacgcagaag
     8041 ttatcaagta cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa
     8101 gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag
     8161 cgtcctttgg ggttcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt
     8221 tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag
     8281 cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcagc
     8341 gcgcgtgggt agcggcaacc tggccggcgt tgcgctggca attaccgccg gtggacctgg
     8401 agccgtcttc tggatgtggg ttgccgcatt tatcggcatg gcgacctcgt ttgccgaatg
     8461 ttccctcgca cagctttata aagaacgtga tgccaatggg cagtttcgtg gcggaccggc
     8521 atggtatatg gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt
     8581 gctcatcgcc tacggcataa ttttcagcgg agttcaggcg aacgccgttg cgcgagccct
     8641 gagtttttct tttgattttc ccccgctggt gacaggtatt attctcgctg tctttgctat
     8701 gctggcgatc actcgcggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt
     8761 gatggcgata atctgggtac tgaccagcct ggtgatttgc gtaatgaata tcgggcaact
     8821 tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg
     8881 cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc
     8941 caatgaggcg ggaatggggt cgacgccaaa cgcggcagcg gcagctgcgt cctggcctcc
     9001 gcatccggca gcgcaaggaa ttgtccagat gattggcatt tttatcgaca ccctggtcat
     9061 ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct
     9121 ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct ggggtgcgga
     9181 gtttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta
     9241 tgccgaaaac aatctcttct ttttacgcct gaacacccct aaagcgatct ggtgtctgcg
     9301 gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agtcttccgc tgatgtggca
     9361 actggcagat atcattatgg cctgcatggc tattaccaat ttgaccgcca tattactgct
     9421 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     9481 cccggtgttt gatccgttgc gttatccgga gatcggtcgc cagctttctc cggacgcgtg
     9541 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     9601 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     9661 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     9721 ctcccagcag ctgatccatg aagcgcggaa actgacgccc ccgcagatta gctccctgat
     9781 gcgcatcagc gataaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     9841 tttcacaccg gaaaatgccc gccaggcaat tctggcgttt aaaggtgatg tctacactgg
     9901 cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     9961 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
    10021 aatggggata cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
    10081 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtag tgataaatct
    10141 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
    10201 gccagtgttc ctcgatgaga agaacggcaa atttaagata atcagcttct acgctaagaa
    10261 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
    10321 aactggtttt aatagcgaag gttacttctt tgatgaagct tcctccagca atggcgaact
    10381 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgg caatccggca
    10441 ttgatattta gcgatgatgt ttgtcaggcc ccggacgatg atcgccacga tgatcattgt
    10501 gcttgttatg gcgcggcgat ggcggcggtc catatgggtg ccagcgattg cctcgccatt
    10561 cataatgttg tttccaccag ccgtggtcgc gccagtgacc gccatcccag taatagccac
    10621 gattatcacg atcgcctatc tgtaatttta ctgacgggac tagcgtaatt tccgcagcct
    10681 gcgctgccat gggagcgaac agaaccaggg aaagtgcgag tacgatagat tgcatctttt
    10741 tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg
    10801 agaacggcag ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat
    10861 ttctcctttt tccctgtcgt ttttctccat attttcttca taaaaaagcc gggctgcata
    10921 aaagcaaacc cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt
    10981 tacgcaacgc agcaaaatcg gcgggcaggt tatgcgaaag caagggtaaa tcagcacgtt
    11041 ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa
    11101 attttgccgg atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac
    11161 gcagcgcacg ataagctacg gcagcgtgcg gctccgaggt atagcccagt tcttttaact
    11221 cacgcattgt ctgttgcgtg gtttcatcat ccacggctgc ataccccagc tctttcagtt
    11281 gccagatttt gcggcggaac aactcttcca cacgcggcca gttgtttggc tggctaacat
    11341 ccatcgcatt ggataacgtc gcctgagtcg ctttgggtga ccactgaccg ccgtgcagga
    11401 aacgtggcac ggtatcattc acgttggtcg cagcaataaa acgcttcacc ggcaggccaa
    11461 gagatttcgc cagcaggccc gctgtcaaat cgccgaagtt accgctcggc accgagacaa
    11521 ccagctggtt gcgcgcttcc tgcggcaact gcgccacggc ttcaaagtag tagcaaatct
    11581 gcgccagcaa acggctgatg ttaatggagt tagcagagtt tagccccagc gccactttca
    11641 gctcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga
    11701 tggcaacagt ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga
    11761 ttttgcctcg tggatagagg ataaccactt tcacattcgg taaaccgtag aaagcatgag
    11821 ccactgccgc tccagtatca ccggatgtcg cggtcagaat ggtcactggc ttatcgcccg
    11881 caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg
    11941 gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga
    12001 aggcaaacgc cgcacgtacg cgctcttcca ggatttcctg cgggatttca tcaccaataa
    12061 acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt
    12121 cagtcaggct gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcctaacc
    12181 cctgggttac ggcttgcgca aagctgacct gctcattgtg atctttcaga ttgtagagtt
    12241 tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ctggcaaata tgaacaaaac
    12301 cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctga gcggtatccg
    12361 gcttgtcaca cagagcgaac aaggtcgggc cggagccgga gataccgctc gcaaccgcgc
    12421 cgatttccgc aaccgcctgc cgcgcctgcc ggaagccagg caataaccgt tcacggtagg
    12481 gttcagcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg
    12541 cgtgaatgaa tcctgccaaa tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg
    12601 ccggtaaaat agcccgggct tctgccgtcg agactttaat ccccggatac gccagcaccc
    12661 acagccactc atcaaaccct ggcacttgct gactgatgat gtcgttttct tcaatcatca
    12721 actgcatacc accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac
    12781 gcccttccaa ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccgcagt
    12841 gttcattcat cgccatcagc gccgcgacca ctgaacaggc gctggagcct aagcccgaac
    12901 cgatcggcat attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc
    12961 aaaaacgctc ccagcactga taaacgatat tttcccgtgg ctctgacggc agcttatcgg
    13021 caaagcgtcc gaggttgttg agactgaatg tctctgctgc ctcaaccgtg actacatctc
    13081 cgagcaatgc accatcaaca ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca
    13141 tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag
    13201 agggtacgta gcagatcagc aaagacaccg gcagctgtaa cgtcattgcc cgcaccatat
    13261 ccgcgcagta ccaacggcag cggctgataa tagtggctat agaaggccag ggcgttttcg
    13321 ccatttttca ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcggcag
    13381 acgccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc acgagccttc
    13441 gccacacgcg cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca
    13501 tcaccctcgg cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccaactcc
    13561 agttcacgtc ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa
    13621 agatcatctc gcgggtccgg ttcggtataa cccatttccc gcgccagtgt ggtcgcctcg
    13681 gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg
    13741 ccggagaact tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcaataacc
    13801 ggtaatccag ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca
    13861 taacgcaact gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtaaca
    13921 acgtggaaac cttcgcgcag gaagtcggca tattgatctg ccacagcctg gctggaagta
    13981 cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg
    14041 agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag attaaggcca
    14101 tgtacattgg tgagcagtgc cttcgagtta gcaacaccgc agacacgtaa gtcgatatgt
    14161 ttattcttca accagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca
    14221 ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta
    14281 acgcgcacgc cagtggtcgc atcatcgtta ttgaccacga cagagattga gcgttcagaa
    14341 gatccctgag caatggcgac aatattgatg ttggcgcggg ccagcgcggc aaagaatttc
    14401 gccgagatcc cacgtaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt
    14461 tccgtcaccg ccaacggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc
    14521 attgcccgtt cagctcgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg
    14581 gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacatcgc tgcaaagacg
    14641 cgcgccgcca tgccaaccat ccctttcatc cccgggccgg aaacgctgaa cattgccatg
    14701 ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacggct ggcaccaatg
    14761 agcgtacctg gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg
    14821 gcgatggggg taatggtgcg ggggtgaaga actttagcgc cgaagtaaga aagctccatc
    14881 gcttcctgat aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cggatcgcag
    14941 gtataaacac cgtcaacatc cgtccagatc tcgcaacaat cggcgcgtaa acaggccgcc
    15001 agcaccgcag cggagtagtc ggaaccgttg cgtcccagaa ccaccagctc gcctttttca
    15061 ttaccggcag tgaaaccagc catcagcacc atgtggtcag ccggaatgcg gcttgccgca
    15121 atacggcggg tggattcagc aatatcaacg gtagattcga ggtaatgacc cactgccagc
    15181 agtttttcga ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc
    15241 ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg
    15301 cactgcccca acaaactgat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg
    15361 acgaaagttt tcaattgtgc cagcggaaat cccggctggg cggcggcgag tcccgtcaga
    15421 agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaata
    15481 gttttttcaa tcatcgctac cagatggttg gtaattttgg cgggggcaga gaggacggtc
    15541 gccacctgcc cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct
    15601 gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct
    15661 ttggtcgaaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg
    15721 cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg
    15781 cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg
    15841 gttaaagtat ttagtgacct aagtcaataa aattttaatt tgatcacggc aggtaaccag
    15901 ttcagaagct gctatcagac actctttttt taatccacac agagacatat tgcccgttgc
    15961 agtcagaatg aaaagctgaa aatcacttac taaggcgttt tttatttggt gatatttttt
    16021 tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg
    16081 cgttgttgta accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat
    16141 gtcatggctc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc
    16201 gccggttgtt gtattaatgt tgctaattga tagcaataga ccatcaccgc ctgccccaga
    16261 ttgagcgaag gataatccgc caccatcggc acaccagtaa gaacgtcagc caacgctaac
    16321 tcttcgttag tcagcccgga atcttcgcga ccaaacacca gagcggcatg gctcatccat
    16381 gaagattttt cctctaacag cggcaccagt tctactggcg tagcgtagta atgatatttc
    16441 gcccgactgc gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcagctagt
    16501 gtcttgaaaa ctttaatatt atcaataata tcaccagatc catgtgcgac ccaacgggcg
    16561 gctggctcca ggtacgcctg actatcgaca atccgcagtt cgctaaaacc catcgttttc
    16621 attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcaaccag aataatcgtt
    16681 atacgcatat tgccactctt cttgaccaaa taaccgcgaa cagggtgatc accgtcaact
    16741 tattacgcgg tgagaattta caaattctta acgtaagtcg cagaaaaagc cctttactta
    16801 gcttaaaaaa ggctaaacta tttcctgact gtactaacga ttgaattgtt aaaaaatgct
    16861 acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgat aatt
//
LOCUS       DAPQZD010000100        16854 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPQZD010000100 DAPQZD010000000
VERSION     DAPQZD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.100"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            60..1109
                     /gene="allD"
                     /locus_tag="RO454_003179"
     CDS             60..1109
                     /gene="allD"
                     /locus_tag="RO454_003179"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDY0934973.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1131..2366
                     /gene="allC"
                     /locus_tag="RO454_003180"
     CDS             1131..2366
                     /gene="allC"
                     /locus_tag="RO454_003180"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDY0934974.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2377..3162
                     /gene="allE"
                     /locus_tag="RO454_003181"
     CDS             2377..3162
                     /gene="allE"
                     /locus_tag="RO454_003181"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDY0934975.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVYGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="RO454_003182"
     CDS             complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="RO454_003182"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDY0934976.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4458..5759)
                     /locus_tag="RO454_003183"
     CDS             complement(4458..5759)
                     /locus_tag="RO454_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDY0934977.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5815..7176)
                     /gene="allB"
                     /locus_tag="RO454_003184"
     CDS             complement(5815..7176)
                     /gene="allB"
                     /locus_tag="RO454_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDY0934978.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7236..8690)
                     /locus_tag="RO454_003185"
     CDS             complement(7236..8690)
                     /locus_tag="RO454_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDY0934979.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(8860..9738)
                     /gene="glxR"
                     /locus_tag="RO454_003186"
     CDS             complement(8860..9738)
                     /gene="glxR"
                     /locus_tag="RO454_003186"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDY0934980.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9838..10614)
                     /gene="hyi"
                     /locus_tag="RO454_003187"
     CDS             complement(9838..10614)
                     /gene="hyi"
                     /locus_tag="RO454_003187"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDY0934981.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10627..12408)
                     /gene="gcl"
                     /locus_tag="RO454_003188"
     CDS             complement(10627..12408)
                     /gene="gcl"
                     /locus_tag="RO454_003188"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDY0934982.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(12498..13313)
                     /gene="allR"
                     /locus_tag="RO454_003189"
     CDS             complement(12498..13313)
                     /gene="allR"
                     /locus_tag="RO454_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDY0934983.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(13391..13873)
                     /gene="allA"
                     /locus_tag="RO454_003190"
     CDS             complement(13391..13873)
                     /gene="allA"
                     /locus_tag="RO454_003190"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDY0934984.1"
                     /translation="MKLQVLPLSQKAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14103..15029
                     /gene="allS"
                     /locus_tag="RO454_003191"
     CDS             14103..15029
                     /gene="allS"
                     /locus_tag="RO454_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDY0934985.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15098..16204
                     /gene="mnmH"
                     /locus_tag="RO454_003192"
     CDS             15098..16204
                     /gene="mnmH"
                     /locus_tag="RO454_003192"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDY0934986.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
     gene            complement(16256..16840)
                     /locus_tag="RO454_003193"
     CDS             complement(16256..16840)
                     /locus_tag="RO454_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002948331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HDY0934987.1"
                     /translation="MSLLAPYDEWYFNFFYPNSLPAQVTYVELLDTDGIYYQFRTLDS
                     TIPVSTTIGQWQENLSGGMTAFNKAKNPPMSIHFCWDSVIDKKEYETWITLAGDVWKL
                     MLTPYSPPGGGSEKYYLRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEK
                     MEMCNGRSAYKHGYSYPESTKNFIKDKKYPYGNW"
BASE COUNT         4410 a         4410 c         4007 g         4027 t
ORIGIN      
        1 tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga
       61 tgaaaatcag tcgggaaaca ctccaccagc taatcgagaa taaactctgc caggctgggt
      121 taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga
      181 tccactctca tggcgcggtg cgcgttgaat actacgccga acgcatttca aaaggcggca
      241 ccaaccgcga accggagttt cgtcttgagg aaaccggacc gtgctcggca attttacatg
      301 ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg
      361 cccagcaaaa aggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct
      421 cttattttgt gcagcaggct gcccgcgccg gattgattgg catttcgctg tgccagtccg
      481 atccaatggt ggtgccgttt ggcggcgcgg aaatttacta cggcactaac ccactggcct
      541 ttgccgcgcc gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg
      601 catggggaaa agtactcgac gcccgctcgc gcaatatgtc tatcccggat acctgggcag
      661 tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg
      721 ctgggccaaa agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg
      781 gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt
      841 tggggcaatt acatatagtt attaacccga actttttctc ctccagcgaa ttattccgtc
      901 aacatcttag ccagaccatg cgcgaattaa ataccattac ccccgcgccc ggttttaatc
      961 aggtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca
     1021 tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat
     1081 acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac
     1141 atttccgtca agctatagaa gaaacgctgc cctggctttc ctcttttggc gctgacccag
     1201 cgggtgggat gacccgttta ctttattcgc cggaatggct ggaaacccag cagcagttta
     1261 aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg aatttatacg
     1321 gtcgcctgaa tggcaccgaa tatccacagg aagtggttct gagcggttcg catatcgata
     1381 ccgtggttaa cggcggtaac ctcgacgggc aattcggtgc gctggcggcg tggctggcaa
     1441 ttgactggct gaaaacgcaa tacggcgcgc cgttacgtac ggtcgaagtg gtggcgatgg
     1501 cagaagaaga aggcagccgc ttcccgtatg ttttctgggg cagtaaaaat atttttgggc
     1561 tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg
     1621 cgatgaaggc ttgcggattt actctgccga acgccccact aactccgcgt caggatatta
     1681 aagcctttgt cgaactgcat atcgaacagg gctgtgtgct ggaaagtaat gggcagtcaa
     1741 ttggcgtggt gaatgcaatt gtcgggcaac gtcgctatac ggtgacgctg aacggtgaat
     1801 caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacggtttac gctttcagtc
     1861 gcatttgcca tcagtctgtc gaaaaagcga aaaagatggg cgacccgctg gttctgactt
     1921 ttggcaaagt agagccgcgc ccgaatacgg tgaatgtagt gccgggtaaa accacgttca
     1981 ccattgattg tcgtcatacc gatgctaccg tgctgcgcga tttcacccaa cagttagaaa
     2041 acgacatgcg ggcgatttgc gatgaaatgg atattggtat tgatatcgat ttatggatgg
     2101 acgaagaacc cgtgccgatg aataaggagc tggtcgccac cctgacagaa ttgtgtgaaa
     2161 gcgaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg
     2221 cgcctcgcgt gccgacctgc atgattttca ttcccagcat caacgggatc agccataacc
     2281 cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc
     2341 tttatcaact tgcctggcag aaataaggag ttataaatgg gatatttaaa taacgtcacc
     2401 ggttaccgcg atgatttact ggctaaccgt gcgattgtta aacacggtaa tttcgcactg
     2461 ttaacaccag acggtctggt aaaaaatatt attccgggct ttgtaaattg tgacgcgaca
     2521 atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa
     2581 aacggcggca accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc
     2641 tctggaaata tcactgccaa agccgaaggg aaaacatttg ccttaagcga aggtggctat
     2701 ctttattgcc cgccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa
     2761 atctttttat ataagcgtcg ctatattccg gtagaaggtc atgcgccgtg gctggtttat
     2821 ggcaatgcca gcgaactgga acgcattcat tatgaaggca tggacgatgt tattctgctg
     2881 gattttctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcacca
     2941 ggtgccagcc acggttatat cgaaacccac gttcaggaac acggtgccta tattctttcc
     3001 ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc
     3061 tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga agcgttcagc
     3121 tatatttact cgaaagattg caaccgcgac gtagagattt aagtcatacc actcgcctga
     3181 ttatttttag ctatgttgga ttttgccacg gtttattccg tggctttttt tgtagtcaat
     3241 tcaggtaaaa aaagatgcga tataaatcgc atccgcttta aaggtaatag tcagttttta
     3301 attccctgac ctattttaat ggcgcaggca atatttcgcg cactgttgaa gagattggtt
     3361 tcaccgctgg cgagcacttc ggctaaagga gccagacgtg gcaaaatgct gaataccgca
     3421 tcaatgccgt actggtgcac cacttccacg ccatcaccca atacgccagc aatcccaatc
     3481 accgggacat taaactgctt cgccaccgat gccacaccca gcggcgcttt accgcctgcc
     3541 gtttgcgagt cgatgcgccc ttccccggtg atcaccagtg ctgcaccctg cactgcctgc
     3601 gcaagattga ccgcattcaa cacaatctca atacccggtt taatatccgc attgaggaat
     3661 accgccgccg caatgcccat accgcccgcc gcgccaccac ccgccatctg gcagacgttg
     3721 atttcagtta gctgttgtaa aacacgggcg taattttgca gcccctgttc cagctcttcg
     3781 accatctccg gcgttgcccc tttttgcggg ccaaacaccg ccgccgcgcc gcgtgcccct
     3841 accagcgggt tatcaacgtc acatgctact tcaatatgac agttcgccag acgcggatcg
     3901 cattcattca tctcaatact tgccacgcgc gctaaattgc cgccgttagc cgccagcggc
     3961 tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg ccatgcccat accgccgtcg
     4021 accgtcgcac tgccgccaat gccgagaata atatggcgaa tgccattatc cagcgcatgg
     4081 agaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt ttcaggcacg
     4141 accagcatca ggccacttgc tgctgccatc tcaatcaccg ccgttttccc gtcgccggta
     4201 atgccataaa aagcattcac tttttcgccc atcggcccgc agacttcaag cttcacgatg
     4261 ttaccgtccg tcgcggcgac catcgcctcc accgtccctt cgccgccatc cgctatcggc
     4321 aaacagatat agtgcgcatc gggaaatatg gtcgaaaacc cggctttaat tgcctgacaa
     4381 catttttctg cacttaagct ctctttaaaa gagtctggcg caatgacaat cttcattatc
     4441 aatccttttt caacaaatta ttcgatgcgt tcccacggaa taagattatc cataagaaca
     4501 gccagtaaaa ttccgaccag taagccgtta ctgagcagag gacgaagcgt taatggaagg
     4561 tcttgcagat acacaggcgg taatgccatt aaaaatatgc cgacaaataa cggcaatgcg
     4621 agtcgataaa tattgcgagc ggtaaacgtt atttgctggc taaacaccag cgcggaaaag
     4681 agtaaaggca gataagaaac cagcatgacc gcactactca ctggtaaagg gatactgcaa
     4741 aacagtcgcg tgagtgccgg aaccagtgcc accagcaggc aaataacgct gccataaata
     4801 aacgaacgcc gcgagtaatc gcctgtctgg gttaataaac caatggatga aacaaacggt
     4861 gaaaatggaa ttactgccag cggcacggtt atcagtgtca taaatccggt cgccacaaag
     4921 ctgcgacgat aacgcgtatt ccccgcgccc tgctgcggat aaaaaacatc cgtgccccga
     4981 atcgcaccgt aggtattgct gatatttacc agacctgtaa tcaccgccgt cagaataatc
     5041 cccggcgaca aagcaccgcc actgccgagc gggaaccact gccagtgcaa ctcaccggag
     5101 agcgaatgcg aagaaggaaa gcaaaagtac cacaacaacc agccagttat cgtgccgacc
     5161 agcaggccgt aacgggcaaa acgttgtggc aggaaaataa tcatcgccag taccaggcac
     5221 atcaccgcca ccgagagcgc gaacggcggt aattgaattt taaaattcgg ggcggctatg
     5281 ccaaacggca gcccgagcat acctttgaaa aagatagtgg tcagttgcgc acccagcatc
     5341 aacataaaca agaccatcac cgacggcgta aataaccgtg ccaggcgatg gcctaatccg
     5401 ctaaaaccaa tcaacatcgt cagcacgccg gagagcgcaa tccccaccgc cagactggta
     5461 gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa gggtgatagt caggatggtt
     5521 ccccaccaca ggccgcccgg cccttccata atagcgcgac gatgaccgca gaacgcctga
     5581 gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg tgagcgtaag caggctactt
     5641 tgcggcaact gaaaagcaga aagtagcgta ggaggaacca caaccgtgtt gcaaaaaata
     5701 aagaaaaacc actggaatcc tgataacagg ctttcgcggc ccactgcaaa attgaacata
     5761 aacacacctt aaccggagaa tgcccgccac aaggacgggc attgcagggc cagattactg
     5821 ctgatgttta aggataaatt gacctttcgg cgcaacaggg aagccctgtt cgatgtcgta
     5881 aatcacatca ccacgtaaga tggttttcgt gatacgcgcg ccaatagtac ggccaacata
     5941 cgggctgact ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg
     6001 ctgaatgaag acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc
     6061 gaaaatatct gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg
     6121 tttctgtacc gcttcatcga acatcacgtc catgcagctt tgcagaccag cgataccgcc
     6181 ccacgctttc atgatgttac cggctttcat ttccggcggg catggagagt ggtcggaaac
     6241 caggcagtct atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc
     6301 gcggatcggc ggtgaacact tcgccagagt accgatttct tcgaactgat cggtatccag
     6361 cacaaagtaa tgcgggcagg attcacaagt aacatcctga ccttcctgac gtgcacgagt
     6421 cacttcctca acaccttccg ggctgctgac gtggcaaacg tgcagacggc aaccagcgac
     6481 tttcgccagg tacagtacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc
     6541 cacatagtca tgggcagtta cgcgaccttc acgcttcgct tcttcgccca gtgcgtcaca
     6601 gatcagcgcg ttttcgcagt gcaccagcac tggctggccc agttcgccca gcttctgcgc
     6661 gcctttgaag aattgccagt cattgacgtc acggaagtcg ttgtcgatac cgcgatcgcc
     6721 acaggtcgca acgaagcatt tgaaaccgac aacacccact tcatccaact catgcagacg
     6781 gtcaatgttg taagacacca ggccaccgag ttgtgccgca tcgatagtca gctttccttt
     6841 agcggcatcg aacttcagtt caattgaggc gcggtcaacc gttgcaggca gctggttgag
     6901 cggcatttcg atcatggtgg tgataccacc ttttgctgct gcgcgagtac cggtttcata
     6961 accttcccag tggctgcgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg
     7021 cgaaaccacc agaccagacg catccataac gtcttttgca tcgcccagat cctggccgat
     7081 agcagcaatt tttccgtctt taacggcgat atctacaacg cgagcttcgt tttctaaaat
     7141 aacggtgccg tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga
     7201 ttggttttta actcaatgtt caatatcgtg attaattaac ctatggtttt ttgttctcct
     7261 gttttttctg ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg
     7321 ccgacaaacc atgaaacacg tgataacggt tccataaagg gaataaactt accgccaaga
     7381 gataaaataa cggccaccag agttactgaa aacgcggtga ggttaaaacc gttatcgtaa
     7441 tatttataat cgccaggtgc ggtgtacagt tcatcaagat taatttgccc acgcatcacc
     7501 acaaaataat gcgccatcat cacaccaatt accggaccaa gcattccgcc gataatatcg
     7561 aggaacagat aaatactgtc ctgattttcc attaatttcc acggacagat cagcaggctg
     7621 ataatactgg caatcagtac gccgttttta taggtcagtt tcgtcggtgc aatggcggca
     7681 atctgataac cagccggaat aatattaccg gtggcgttag tagagatagt cgtcatcaga
     7741 ataaccagta ccgcaaagaa cgaggcgaac aggctgtccc aacgctgaac aatatccagc
     7801 acgttccagg tgtcagcgcc gtaatgaata ctggctccgg caataataca taccccggcg
     7861 accgcaaaca gaatataggc cacaactaaa cccagcgttt gccccagcgc ctgctcacga
     7921 aacgagtggg cgttttgcgt aaagtcggat gcgctcaccg ccggtgctgc ccagaccgca
     7981 accaccgcgt ttatcaccac cagaaacagg aagccactgt tttctgcttt ctgaataccg
     8041 cccggaatgt agtcaaagat tggaccgatc ccgaccagcg aaatcgccca aatcgccata
     8101 ccgccgaaaa cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttt
     8161 ccgccgccaa aaccaatacc aacgttaacc agccagaaga gtaagaaagt aattaatccc
     8221 ggtagagaaa ggcctaacag cgtgaaatcg ccaccgagag tcaaaaatcc cggccagatt
     8281 ttgccaatca gaatcaagca ggccagtgac cccgcgtaac attgcaggcc aaaccacata
     8341 atggcagcaa taccgcctct taataatccg ggaaacagtg caccacgtac gccgtaagaa
     8401 gcacgcagga tcatggcaaa cggtacgccg tatttactgc ccgcagcacc gtttaatacc
     8461 attaccgcgg caatgaaaaa ggcactgagg ataattgcca gcataatact aaaggtagac
     8521 aagccgagaa taaaaaagcc gccaaccatc acataattgg gaacgttatg aaccgaaccc
     8581 atccataagg taaaatagtt aaatgttttc caggtccgct gactttgcgt tttcggcagt
     8641 agatcttcgc tatagccgcg ttgctggaat agttttctct gatgttccat aaatccctct
     8701 taatttcacg tatcgaataa cgttgtttta atctagctat gaggaacaaa taaaataaaa
     8761 atattatttt taatactgaa gcaacatctt tggctgcttt ctttttttaa tattgctggc
     8821 cgattcattt tctgatatcg gccattttta ttgcgggtat caggccagtt tatggttagc
     8881 cattaattcc agcgcctgca ctaatgcaga gtgatccaac tggctgccac cgtttgccgc
     8941 acaggtatta aacaattcct ggcaggtcgc ggtgtttggc aggttcagcg ccagtgcttt
     9001 cgcactttgc agtgccaggt tgagatcttt ctggtgcagc gcaattttga aacctggatt
     9061 aaaggtgcgt ttaatcatgc gctcaccgtg aacttccaga atacgtgagg aagcaaagcc
     9121 gcccatcagc gcctggcgca cacgtaccgg gtccgcaccg gcttttgaag caaacaacaa
     9181 ggcttcagaa accgcttcaa tattgagcgc cacgataatc tgatttgcca ctttgcaggt
     9241 ttgaccgtcg ccgttaccgc ccacgagagt gatattttta ccgagcagtt caaacagcgg
     9301 ttttacacgt tcaaatactg cttcatcacc gccaaccata atcgataacg tcccttcacg
     9361 cgcgccgatt tcaccgccgg agactggcgc atcgagataa tcgccgccca gttcattcac
     9421 ctgacgagcg aaacgtttag tttcaatcgg ggaaatggag ctcatatcaa caatggtttt
     9481 gcccttcagc gaggctttgg tacaaccatt ttcaccaaac agaacgtctt caacctgtgg
     9541 cgtatccggc accataataa aaatgatgtc cgatgcatct gttacctggc gcgcagtttc
     9601 aacactaacg gcgcccagtg acagtaattc atcagcaacc ggaccaatgg ttgtgacatg
     9661 cagttgatga ccggcacgcg ccagattaat ggccatcggt gtacccataa tgcctaagcc
     9721 aataaatccc agtttcataa ttaacctctt ttaaatttcg cttttcctga attcagacaa
     9781 cacgatcccc tgccaactta gcggcctaaa aaagcattgt ctgaatagcg ttacgtttta
     9841 acggtacggg tccatccagc gtaaaccggc ttccgtggtg gtttggggtt tatattcaca
     9901 gccaacccaa ccgttgtagt cagaattttc aattaccgta aagagatatt cgtaattaat
     9961 ttctccggtt cccggttcgc cgcgatgcgg attatcagca atttgcaggt gaccaatttt
    10021 atcggcccac tgagtcatgg tattggttaa ttcaccttcc atccgctgca tgtgataaat
    10081 gtcatactga attttcaaat tgcagcaacc aacatcgtca atcagtttca gtgcctgccg
    10141 ggttccggtg agatgaaaac caggaatatc aaaatggtta ataggttcaa tcagtaataa
    10201 aatatcttct ttcatcagca tattcgcagc ataacgcagg ttttctacaa gcgttgtgtg
    10261 aatctgttca ctgctgaaac cagtcggtgt tttaccgacc agacagttaa tttttttatt
    10321 acccagcgca cgagcataac gaatcgctgc tgctacgcca tcccgaaact cttcttcacg
    10381 gccaggaata caggcaatac cccgctcgcc cgccgcccag tcacctgccg gtaaattgtg
    10441 cagcgtatgt tcaagtttat tactcgccag cacctgtttt aattcttcaa tgtcgtagtc
    10501 ataagggaac ataaattcaa cgccgcgaaa accacactgc gcagctttct caaaacgggc
    10561 gagaaaatca tattcaccaa ataacatcga taaattagca gagaaacgta acattattta
    10621 tctcccttat tcatagtgca tgaaacaggt ttcagtcggt gcatccgctg cgttatcggc
    10681 gatatcttca aattccatga cgttatccag ttcactgccc atcgaaatat tagtcacacg
    10741 ctcgagaata acttccacga ctaccggtac ccgatattgc gccattaaga ttttcgcctg
    10801 ttcaaaggct ggtgcaatat cttccggttt gaaaacgcga attgctttac aacctaaacc
    10861 ttccgctact tttacgtggt cgacgccata accgttcact tcgctggagt taatattttc
    10921 gaacgcgagc tgtacgcagt aatccatatc aaaggcacgt tgcgactggc gaatcagacc
    10981 cagataggcg ttattgacca gtacatggat gtacggaatg ttgaactgtg cgccaacggc
    11041 taattcttca atcaggaact ggaagtcgaa gtcaccggaa atcgccacca cattgcgctc
    11101 tggatcagcg gcacaaaccc ccagcgccgc cggaatcgtc cagcccagcg ggcctgcctg
    11161 cccacagttg atccagtggc ggtctttaaa gacatgcagc atttgtgccg cagcgatttg
    11221 tgacaggcca atggtggtga cataacaaac atcgcggcca aaggctttgt tcatctcttc
    11281 atacacgcgc tgcggtttca ccggcacgtt gtcgaagtgg gttttgcgca gcaaagtgcg
    11341 tttgcgctgc tggcagtcgg cgacccactc tttacgacac ggcagacgac cggctttttg
    11401 catctcctgc gccacttcaa ccagcagcgt cagcgccgct ttagcatcag agacaatgcc
    11461 aagatccgga cacagcacgc ggccaatttg cgtcggctca atatcgatat gaacgatttt
    11521 gcgcccttcg gtgtatttct ctaccgaacc ggtatgacgg ttagcaaaac ggttaccgat
    11581 accaaacacc atgtcggacg ccagcaacgt tgcattaccg taacgatgcg cggtttgcag
    11641 gcccaccatc ccggccatca gttcatgatc gtccgggata cagccccagc ccatcagcgt
    11701 tgggatcacc ggaatgctgg tcagttcagc aaactgttgt aacagtacag ctgcgtcagc
    11761 attaattacc ccgcctccgg caacaatcac cggacgttcg gcctggatta acatttctac
    11821 agctttttcg atctgcatac ggctggcagc aggtttgtag accggcagcg gttcgtacat
    11881 atcaggatca aactcgattt ccgcaacctg tacgtcgaac ggtaaatcca ccagtaccgg
    11941 acccggtcga ccagaacgca tcaggtgaaa cgcctgttgc agcacacgag gcaccagcgc
    12001 cgcttcacga actgtaaccg ccattttgct gaccggttta gcaattgctt caatatcaac
    12061 ggcctggaaa tcttctttat gcagacgggc gcgcggagcc tggccggtaa tacacagaat
    12121 aggaatggaa tcagcagatg cggaatagag cgcggtgatc atgtccgtgc ccgcaggacc
    12181 ggaagtcccc agacatacgc cgatattccc tgccgttgcg cgggtataac cttccgccat
    12241 gtgcgaagca ccttccacat gacgcgccag aatgtgacga ataccgccgt gcttacgcat
    12301 cgctgagtag aacggattga ttgcagctcc cggaacgccg aaggcggtgg tgataccttc
    12361 tttctccagc acatacattg ccgcgtcaac ggctctcatt tttgccattt tattcctacc
    12421 tctattaatt ggaaaaattt tccaactttc aaattttgtc aaaaaaaccc gctccgcaga
    12481 gcgggatacg acagacacta tagatgtgct ttcagtccca acgccgtgct gatatcgcgg
    12541 gcggtgtctc tgaccagctc accctgactg acaaaacgat cttctgtcag tcttgatgaa
    12601 ggcccggaga tggagatagc ggcaacaaca ctacctacat catcgtaaat tgctgaagct
    12661 atgcaattca ggccgacaac atgctcttct ttatctacgg tatagcccag ttcacgcgct
    12721 tgttccaggt ccttcagcaa ggtgggcata tccacaagtg tcgttggcgt aaactgctgc
    12781 aaaccggttt gcagaatgat gctcatcaac tcttcttccg ccagcggata aagcagcgcc
    12841 ttgcccgctc cggaagcatg cagcggcaga cgactgccca gcggcgcaca catcctgacc
    12901 atcgatttac actctaactg accaattaat accgcttcat tgccgttacg gatcgcgaca
    12961 ttgaccgttt cgccggaaag taacatcagg cggcgcataa acggcccggc gacggagagg
    13021 acatcgcggt tatggatgta cgctgcaccg acgttaaaga cacctaatcc tatatgccac
    13081 cagcctaact gattgtcctg atagacaaaa tccgctgcct gtaaaacctt cagcaagcga
    13141 aaggtcgtgg agagcggcaa atccagattg agagagatat cgctaaccga cgaacttccc
    13201 ccactttttt ccagatattg cagaatcgca attccccgct ctaacgcctg tgcgcccttc
    13261 tgtgctacag gctccgcctg tcctggcctg ccgcgccgtc taacttccgt catacctacg
    13321 ttaactccga cctacaaatg agcataaaca atgaacaatt cgtttatctt atgcaagtcg
    13381 gttgcaggcg tcacgcaaaa cagagttcct gttcaggaat actttcaaca tcacagttgt
    13441 cactgccgcc gcgatcgatg gtcagaaaat cggtgacgcg ctgccaggcg aaaagtgggt
    13501 gatgccagac gttacgatgg taattcactc cctgttcgcc attggtaata aacgcccgca
    13561 gggttgacag gtctggtttg tcgtcaccta acgccaccac caccacaaac acttcacctt
    13621 tcatcgggat aaacgcctgt gtacccagcg gatgacgttc gagttcgtga atggtcagcg
    13681 gcagattcgc cggttgcgcg cggttaatgc tgataagcgt acggtcttgc tcaagaatct
    13741 caaccagcgc caaatcgtgg taacgctcca ccagtccatt gttaatatgg aaaaaatccc
    13801 gtttctgcgt ttcgattacg tcgccataag cactaaaggc tttctgactt aacggtaata
    13861 cctgaagttt catctttttg tccttgtaac ataattccgt tcaaccaccg ccgaccggtc
    13921 agcttggttt caataacagg caatctaaaa ccttaagaga acaacatcaa cacaaaagtt
    13981 ggaatatttt tccaaaacta gatttagtcc acaaattgaa cagggttatc atgtaaaaat
    14041 actgataaat cagaggataa gaaaatcacg caacttgcgg atttaaaagg ggatacctga
    14101 agatgttcga tccagaaacc ttgcggactt tcattgcggt tgctgaaaca ggtagttttt
    14161 caaaagcggc agaacgatta tgtaaaacca cggcgacgat cagttatcgc attaaacttc
    14221 tggaagagaa taccggagtg gcgctgtttt tccgcacgac tcgcagcgtg acgttgacgg
    14281 cggctggcga gcatctactt tgtcaggcca gagactggct gagctggctg gaaagtatgc
    14341 ccagcgagtt acaacaggtg aatgatggcg tggaacgcca ggtgaatatc gtcatcaaca
    14401 acctgctcta taacccccag gccgttgcca ggctgctggc gtggctaaat gaacgttacc
    14461 cgtttaccca atttcacatc tcccgacaaa tctatatggg cgtctgggac tcgctattgt
    14521 acgaaggttt ttcactggct atcggcgtca cgggaaccga ggcgctggca aataccttta
    14581 gtcttgatcc cttaggatcg gtgcaatggc gctttgtcat ggcggcggat catccgctgg
    14641 cgaacgttga agagccgcta acagaagcac agttgcggcg ctttccggcg gtcaatattg
    14701 aagacagcgc ccgcacctta accaaacgcg tcgcctggcg attgccgggg caaaaagaga
    14761 ttattgttcc cgatatggaa acgaaaatcg ccgcccatct ggcgggcgtt ggcattggtt
    14821 ttttgccaaa atcactttgc cagtcaatgc tcgataatca acaactggtc agccgggtaa
    14881 tcccaacgat gcgccctcct tcgccattga gtctggcatg gcgcaaattt ggcagcggca
    14941 aagcggtaga agatattgtg accttgttta cccagcgcag gccggaaatc agcggatttt
    15001 tagaaatttt cggcaaccca cgcagttaaa aatcaagttt ccttgcgcgt tggcgcgtaa
    15061 aatagcgcgc aagctgattt gaggttcact ttttactatg caagagagac acacggaaca
    15121 ggactatcgt gccctgctga ttgctgatac gcccattatt gatgttcgcg cccccatcga
    15181 gtttgagcaa ggcgcaatgc ccgccgctat caacctgccg ttaatgaata acgatgaacg
    15241 cgccgccgtt ggcatctgct ataaacagca agggtcagac gcggcgctgg cactggggca
    15301 taaactggtg gcgggtgaaa ttcgtcagca gcgcatggac gcctggcggg cagcgtgcct
    15361 gcaaaatccg cacggtattc tctgctgcgc ccgtggcggt cagcgctcac atattgtgca
    15421 aagctggttg catgacgcgg ggattgatta tccgctggtg gaaggcggtt ataaggcact
    15481 gcgccagacc gcgattcagg cgactattga actggcacaa aaaccgatag tgctgattgg
    15541 cggttgtacc ggcagcggta aaacgctgtt agtgcagcaa cagccgaacg gtgttgatct
    15601 ggaagggttg gcgcgtcatc gcggttcggc gtttggtcgc accttacaac cacaacttag
    15661 ccaggcgagt tttgaaaaca tgctggctgc cgaaatgcta aaaaccgacg cccatcagga
    15721 tttgcgcctg tgggtgctgg aagatgaaag ccggatgatc ggttcgaatc acctgccgga
    15781 atgtctgcgc gaacgaatga ctcaggcgac gattgcggtg gtagaagatc cgtttgagat
    15841 ccgtcttgaa cgcctgaacg aagaatattt cttgcgtatg catcatgatt ttacccacgc
    15901 ttatggcgac gaacagggct ggcaggagta ttgcgaatac ctgcatcacg ggctttcggc
    15961 gattaaacgt cggctgggac tacaacgcta taacgaactg gctgcaaggc tggatgcggc
    16021 gctgacaacg caactcacca ccggcagcac cgacggtcat ctggcctggc tggtgccgtt
    16081 acttgaagaa tattacgacc cgatgtatcg ctatcagctt gagaaaaaag cggaaaaagt
    16141 tgtctttcgc ggtgagtggg cggaagtggc ggaatgggtt aagacttatg cctgtggtta
    16201 ttaacccaca aaaatgcttt tccgttcctc tttaataaat tgtgcaactg gttaactacc
    16261 agttgccata gggatatttt ttatctttga taaagttttt tgtactttcc ggataagaat
    16321 acccatgttt ataggcactt cggccgttac acatttccat tttttcacct gaaaccgttt
    16381 ctatcagaat ctgcttatcc gtcaggcgaa tattgggctt gtcaggtttc tccagccaca
    16441 ctcctatttt cccttccgga gccagcccga ttaacagata ccgcagataa tacttttcac
    16501 tgccaccgcc aggtggtgaa taaggtgtga gcatcaattt ccagacgtct cccgccagcg
    16561 ttatccaggt ttcatactct tttttatcaa tcactgaatc ccagcagaaa tggatagaca
    16621 tagggggatt tttggcttta ttgaatgccg tcattccacc actaagattt tcctgccatt
    16681 gtcctattgt tgttgagaca gggatagtgc tatccagagt tctgaactga taatagatgc
    16741 catcggtatc taataattcc acataggtaa cttgtgctgg tagggaatta gggtaaaaaa
    16801 aattgaaata ccattcatca tagggtgcaa gcagtgatat tttttctttc gctg
//
LOCUS       DAPQZD010000101        16850 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPQZD010000101 DAPQZD010000000
VERSION     DAPQZD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.101"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            68..1300
                     /locus_tag="RO454_003194"
     CDS             68..1300
                     /locus_tag="RO454_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDY0934988.1"
                     /translation="MYRDTRREYIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
     gene            1326..2249
                     /locus_tag="RO454_003195"
     CDS             1326..2249
                     /locus_tag="RO454_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDY0934989.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            2304..3257
                     /locus_tag="RO454_003196"
     CDS             2304..3257
                     /locus_tag="RO454_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDY0934990.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
     gene            3306..3743
                     /locus_tag="RO454_003197"
     CDS             3306..3743
                     /locus_tag="RO454_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDY0934991.1"
                     /translation="MRIIPITPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPEGILF"
     gene            3753..5240
                     /locus_tag="RO454_003198"
     CDS             3753..5240
                     /locus_tag="RO454_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDY0934992.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            5424..6188
                     /locus_tag="RO454_003199"
     CDS             5424..6188
                     /locus_tag="RO454_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0934993.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6302..7000)
                     /gene="thiQ"
                     /locus_tag="RO454_003200"
     CDS             complement(6302..7000)
                     /gene="thiQ"
                     /locus_tag="RO454_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDY0934994.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(6984..8594)
                     /gene="thiP"
                     /locus_tag="RO454_003201"
     CDS             complement(6984..8594)
                     /gene="thiP"
                     /locus_tag="RO454_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDY0934995.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQVLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(8570..9553)
                     /gene="thiB"
                     /locus_tag="RO454_003202"
     CDS             complement(8570..9553)
                     /gene="thiB"
                     /locus_tag="RO454_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDY0934996.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9584..9683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9717..11372)
                     /gene="sgrR"
                     /locus_tag="RO454_003203"
     CDS             complement(9717..11372)
                     /gene="sgrR"
                     /locus_tag="RO454_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDY0934997.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLTEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11461..11592
                     /gene="sgrT"
                     /locus_tag="RO454_003204"
     CDS             11461..11592
                     /gene="sgrT"
                     /locus_tag="RO454_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDY0934998.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11699..12304)
                     /gene="leuD"
                     /locus_tag="RO454_003205"
     CDS             complement(11699..12304)
                     /gene="leuD"
                     /locus_tag="RO454_003205"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDY0934999.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12315..13715)
                     /gene="leuC"
                     /locus_tag="RO454_003206"
     CDS             complement(12315..13715)
                     /gene="leuC"
                     /locus_tag="RO454_003206"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDY0935000.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13718..14809)
                     /gene="leuB"
                     /locus_tag="RO454_003207"
     CDS             complement(13718..14809)
                     /gene="leuB"
                     /locus_tag="RO454_003207"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDY0935001.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(14809..16380)
                     /gene="leuA"
                     /locus_tag="RO454_003208"
     CDS             complement(14809..16380)
                     /gene="leuA"
                     /locus_tag="RO454_003208"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDY0935002.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIDKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16472..16558)
                     /gene="leuL"
                     /locus_tag="RO454_003209"
     CDS             complement(16472..16558)
                     /gene="leuL"
                     /locus_tag="RO454_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDY0935003.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         3925 a         4179 c         4449 g         4297 t
ORIGIN      
        1 tttttcttga taaaaatccc gataacaatt tcttttttag tgataataaa atcaaaaagg
       61 aagacttatg tatcgggata cccgtcgtga atatattgtc cggttacttc gcaacaagga
      121 tgagtgcagt gttagcgatt tagctaacta tttttctgtt accaaagaga cgattcggac
      181 agatttagcg tggttgcaaa agcgtggtgt agtgagtcgt catcatggag gcgtctcatt
      241 gaaaaaacat ttaatgcaaa atgcgctttc gcaacaagag tatgtcgata tgagtttgct
      301 tatcaagcaa caacagcagg gtattggcta tttaacctcg cttgatgata aaggacgccg
      361 aatggtaggt aaagtttgtg tgttaggatc atttaacgtc gatattgtgg cgaaagtgta
      421 tcgcttcccg aatgaaggcg aaacgttagt ggccagggaa actaccatgg ggccgggggg
      481 taaaggcgcg aatcaggcac tagccgctca tcgggcaggt gcgcaaatac acttcgcctg
      541 taagattggt cgcgatcagt ttaatcattt tgcccgcaat catattgagt ccattggtat
      601 tggttcattt actttttatg agtctgaact atctaccacc ggttgtgcgg tcatttatgt
      661 aaatgatgaa ggtgaaaaca tgattgctat gtctccaggg gcaaatcatg aattaagtga
      721 taacgatatt attcaacttt ctcactttat agccgagtcc gatgtattca ttgtacagat
      781 ggaaaataat cttgccgcta ctcaactggc attaaaatgt gctcaaaaaa tgcaggtgac
      841 aacgatttta aatcctgcac cgtggtccag tgatgtcgcc acattattac cttttgttga
      901 tattgtgacg ccaaatgaaa ccgaagcatc ggcgatgtca ggtatggtaa ttgacaatat
      961 ttctgatgct gtgaaggctg cgaagcatat ttataatgcc gggcaatgtt cagtaattat
     1021 tacgatggga aaacgaggtg cattaattta tgacgggcaa caatgtgcgc atattccggc
     1081 attttcggca gtcgctgtag atacaacagg tgcgggtgat gcgtttaatg gcgcgctcgc
     1141 cgccgcactg gcgaagggag aatcacttgt taaagcagcc tggtatgcca gcgctttcgc
     1201 ttcgctggca gtagaaaaag aaggggcagc caatatgcct gatatctctc tggtggatgc
     1261 cagaattcag cagcagaatg ttgccattca gactttctga tgtttcactg aacgaaggat
     1321 gaaatatgaa aacaattgaa gggattattc cggtaatgct aacgcctttt acggcggata
     1381 ataaaattga ttacctgggt cttaaacggc taattgactg gtatatcgaa aacggtgccg
     1441 acgcgttatt tgccgtgtgt cagtccagtg aaatgcagta tttaacttta gaagagcgcg
     1501 tggagctggc gagctttgtg gttgaatata gccaacaacg agtaccggtt attgcgtccg
     1561 ggcatatttc tgatgatctg gaacagcaaa ttcaggagct caatgctatg gcggcaacgg
     1621 gcattgatgc gctggtgtta gtgacgaatc atcttgatcc acataacgaa ggtaccacgg
     1681 tattcctccg tacgcttgat gcgctattga tgtcattacc cgaaagtatg gcgctgggtc
     1741 tgtatgaatg cccggccccg tatcgccgct tattaaccga tgaggaactg ctttactgcg
     1801 cacaaagcgg gcgttttcaa atcttaaaag acgtcagttg tgatctacct acagtgacgc
     1861 gacgggtgca attaactcag cacacgccaa tgacgattgt taatgctaat gctgccattg
     1921 cctgggaggc gatgcttgca ggttcgaaag gtttttgtgg cgtctttgcc aactttcatc
     1981 ctgacttgta tcactggctt tggcttgaag gaaaacaaca ccctgaagag gcaaaacaac
     2041 tcgcctggtt tttatcgctg agtgccgtca gtgaaaatat tggctatccg aaaaatgcca
     2101 aaatatttca tcagcagcga ggaacatttg actgtgttca ttgccgggta acggctgacg
     2161 atgtgctgaa aaaatattgg gggctggagg tggtgctaaa acaaattgct gatgccgcag
     2221 atttccagcg tcaacaactc aaatattaat atccgacagc acttgctgtt attaaaaaat
     2281 aattaagaca ggagtctaca aatatgaaac gaaccctgat gagttgtgcc gttgcttcac
     2341 tgctggtttc aggttccgcg ctggctgcat ggccagaaaa accaatccaa atcattgtcc
     2401 cctggggcgc gggaggaaat accgatacgg ttgcacgcct ggtggcagaa gggctgcaaa
     2461 aagagttgaa agtgaatgtc aacgtggtga atcgcacggg aggtgctggg gtggttggcc
     2521 atgatgccat tgcgaaagct aaagcggatg gctataccct gggaattgcg acggtagaaa
     2581 tcaccatgat gcaccatcag gggatgacgg cgttaacgta tcagaactac acgccaatta
     2641 cacggctggc agtgaatctg ggaggcgtgc aagtcgcagc aaattcgcag ttcgaaaata
     2701 tcgtccagat gaccgactac atcaaggcga atccaggtaa gttaaaagca tcgggtagcg
     2761 ggttaaattc cggctggcat ctgaacttga taggtatgct cgacagtatg gggcttccgc
     2821 cttctgccgt gacctgggtg ccttctgaag gttccagttc tgcactgatg gaactggttt
     2881 ccggtggtat agattttaca acgtcatcac caggcgaagc gaaaagtatg gtcgatgcca
     2941 aaatggtgcg taatttagcg accatggcag aaaaatcagc agggctttat aaagatcttc
     3001 cagtattcca acaagcaact ggctataaat atcatttggc gacatggaat gccttagtgg
     3061 ttccggcggg tactcctgaa gaggtctcac aaaaattaac tgccgctatg caggcagtgt
     3121 ttgcgcaggg gaaattacaa gaattcgcta ccaaacaggg tttcgaagtt tacccgctgt
     3181 atggcaaaga gatgacgaca tttatggcag atgaagatgc cagatacgga aaattaatag
     3241 ccaaaatcaa aggttaatca gacagggcgg tgaatatatc gccgccaaat tacagggata
     3301 gcattatgcg cataataccg ataacgccgt tatttctgct gatttttgcc atgtttattt
     3361 ttggctggag cataacgcaa tatggaggca tatcgcagta tggcgctgga tttatgcctt
     3421 gcatcatcag tggattgttg ttgtttttag cggctattga tgtagcgata gatattaaac
     3481 accggcaccg taacaaagtt tctttaactt ttgatgaagt gaaagcactt ttactggtta
     3541 ttacagtggt actactgttt gtattactgt tagatattct cggctttgtg atttgctcat
     3601 ttttgttgct ttttatttta atgaaaatac gaggtgtcag acttttgcat tccgttatcg
     3661 tttcgatatc ggcatctctt attatctggt tcatttttgc cagagtgttg ttggtcgcct
     3721 ttcctgaagg aatcttgttt taaggatgca atatggatat tttgattcag gcactaacgt
     3781 atattaattt gaatgccatt ctggccatta ttgctgctac ggcctttggg ttatttgtgg
     3841 gggccatccc tggattgacc gcaacgatgg cggttgcact aatggtgcct tttaccttct
     3901 ttcttgatcc tattcccgcc ctggcattaa tgattgccgt tggtgcgtca tccatttatg
     3961 ccggagatat ccccggcgca cttttgcgta taccagggac acctgcttcg gcggcttatg
     4021 ttgctgattc aaatgaactg gtcaagcaag ggaaggtgaa tcgcgtattg ggacttgggc
     4081 tggtgagttc ggtcattggt ggcgtgattg gatcacttat tttgatgttg gctgcgcctg
     4141 tactggcgaa atttgccctt aattttagtt cttatgaata cacatggctt tctcttttag
     4201 gcttaacttg cgcgactctg gttgtgggta atcaaccact gaaaggcatg gtgaccttat
     4261 gtttgggatt attactggcg tgtatcggtt atgatcaaat atccggagtt ccacgtttta
     4321 cgtttggtca aatcagcctg ttacaagggg tgaactttat cccggcgatg attggtctgt
     4381 tcgcgatatc cggcgcaatt gaatattatc gtgacaaaat tagccaggta agtggtgttg
     4441 tgccacaaac gcaaacgggg attaatttat tcaagggaat ggggcaggtg atttggtcac
     4501 gaaaattaaa tcttggccga agtagtatta tcggtacgct tatcggagca ttacccggcg
     4561 ctggagcgga tattgctgcg tggataagtt acgccatttc aaaaaaattc tctcgccagc
     4621 aggagttata tgggcatgga tctgaggagg caattgttga ttcctcgagc agtaacaacg
     4681 ccagtctggc ggggagttgg atcccttcat tagttttcgg tattccgggc gattctgccg
     4741 ctgcaattat catcggcgtg ctctatatga aggatatgaa tccagggccg acattatttc
     4801 tgttccaggc agataaactt tatgccgtat ttatcttgtt tctgatcgcc aatattgcat
     4861 tactaccgtt agccacaatt gcggtcagtt tcatcaaacg catcatctgg attgataaag
     4921 cgattctgta cccgattatt ctgattttca gtattgtcgg tgcatttgct atcgataact
     4981 cgggtgcttc tgtcgttgtc atgttggtca tgggggttct tggatactgg ttacaacgta
     5041 aggaataccc ggtttcgccc attattctgg ggatgattct tgggccaatg ttggaaaaaa
     5101 acttattatc ttcaatgatt aagtctaatg gcgaatggct ggcgtttgtt gagcgcccgg
     5161 tatctatggc actggctgtc tgtttctttt tggttgtcgc gttgcaagga cgcaatatct
     5221 accgttcatt tagccgttaa tccgttgttt tcagaccatc caagttgtgg atggtctgcc
     5281 ttcccctaag taaaatcctg cgtgactgct aaatttatac tctaaatgat tcgcgttgca
     5341 ggaatatatt catacaaaga atagattgac ctgaatacca ccactttgca caatccacga
     5401 cgaattcatc aaccgggcac attatgcaag cattgctgga acactttatt acccaatcca
     5461 ccgtgtattc attgatggcg gtggtgttgg tggcctttct ggagtcgctg gcgctggtcg
     5521 gtttgattct gcccggtacg gtgctgatgg cggggctggg agcgttgatt ggcagcggtg
     5581 agttaagttt ctggcacgcc tggctggcag ggatcgtcgg ttgcctgctg ggcgactgga
     5641 tctccttctg gctgggctgg cgttttaaaa agccgttgca tcgctggtca tttctgaaga
     5701 agaacaaagc attactcgat aaaaccgaac atgcgctgca tcagcacagc atgttcacca
     5761 ttctggtcgg tcgttttgtc ggcccgacgc gtccgctggt gccaatggtg gcagggatgc
     5821 tggatctgcc ggtggcgaaa tttattacgc caaatattat cggctgcctg ttatggccac
     5881 cgttttactt cctgccgggg attctggcgg gtgcggcgat cgatattcct gccgggatgc
     5941 agagcggtga gtttaaatgg ttgctgctgg tgacggcggt attgttgtgg gttggtggct
     6001 ggctgtgctg gcggttatgg cgcagcggta aagcgactga ccgtttgagt cattatttgt
     6061 cccgcggtcg tttgttgtgg ctgacgccgt tgatttctgc catcggcgtg gtggcgctgg
     6121 tggtgttaat tcgccacccg ttgatgccgg tgtatatcga tattttgcgt aaagtggttg
     6181 gggtttagag gatagccttg tgcgggttgc ctgatgcgac gcttgctgcg tcttatcagg
     6241 cctacaaaac gcactacctg taggtcggat aaggcgttca cgccgcatcc gacagtgcat
     6301 attaaccttt aattcccaac agcgccgaag cactcgcctt accgctcaac aactcatcgg
     6361 tcttaccctg ccaggcgata cgcccgtcgg cgaccaccac cgagcgcgtg gcgatccgcg
     6421 ccgcatcttc cacactgtgc gacaccatca agagcgtcat tttttgctgt tggcagctcg
     6481 aactcaccag cgtcagcatc tcctgacgta gcgccggatc gagtgcggaa aacggttcat
     6541 cgagcaataa aatgggctgt tcgcgtacca gacaacgcgc taacgccacg cgctgtcgct
     6601 gaccgccgga aagctcgcca ggtaaccgcg ccattaaatt atcaatcccc atctggtggg
     6661 caatcgcgtg cattttcttc tgctgcgccg catttagttt caaccccgga tttagcccca
     6721 gcccgatgtt ctgcgcgacc gtcaggtggc tgaacaggtt gttctcctga aacagcattg
     6781 acaccggacg acgtgacggt ggcgttgtgg tgtgatctac gccgtcgaga gtcagcaaac
     6841 cgctggctgg cgtcagaaaa ccggcgatca aatttagcag ggtgctttta cccgcgccgc
     6901 ttggcccgag gatcgccacc tgctcgccgc gttccaccgt taagctaaaa cgcatcggca
     6961 aatggtggta aagccaggtg atatcagtca gttttaacat ttcgccccgg tattttttca
     7021 atcacggtaa acagcagaaa acagagcagc agcagaatta acgcggtgac cgcgccgtcc
     7081 tggctgcgat aggagccaat ttgctgatag agataaaacg gcagggtacg gaaatcatcg
     7141 ttaccgaaca atgccaccac gccaaaatca ccaatcgaca gcacacaagc aaaggccagc
     7201 gcctgcgcca gtggacgttt cagagcgcgc agctccacca cttttaagcg tgaccagcct
     7261 tcaatgccca gcgactgaca caacatgctg tagcgggcgg tgatatcgcg catcgggttt
     7321 tccagtactt tcagcgcata agggattgcc attaacgcat tggtgaaaat cacaatgccg
     7381 tcagcagatt gtggcaggcc gatagtgttg ttgagcagta aaaagaagcc cgtagccagt
     7441 acaatccccg gcatggcgag gatcaacatg ccgctcatct ccagcgcctg acccgccagc
     7501 attttctgcc gtgcccgaag ttcgcgactg ctccatagca gcatcatggt cagcactacg
     7561 cacaatacgc ctgccgccag cgcaatacgc aacgaggtcc acagcgcctg ccacagcacc
     7621 ggttgtgcca gcacttccgg caactggcga tttaccccat cgacgatcac cgccagtaac
     7681 ggtggcagca acagcagcag cgccagcaca attaacgccg tgtcgcaaat gcgactatgc
     7741 agacgatcgt ccgggtcgcg ccagccttgc agcagtgtgg taccgggcgc aatggcctta
     7801 ctcaatcgct gactcaacag caccagcccg aggcagcaca ccatctggat cagcgccagc
     7861 attgccgcgc gggcaggatc gtagtcgtaa ctcagcgcct gatagattgc cagctcgata
     7921 gtggtcgcct gcggaccgcc gcccagcgat agcacggtgg cgaagctggc gaaacagagc
     7981 ataaagataa gcgcagcaac cggcgggatt tgtcgccgta accacggcca ttcgacgaag
     8041 cggaagaagt gccagccgcg catcccaagt tgtgcggcaa gctgacgttg ttcgccggga
     8101 atgttttcca gtgcctggag taataaacgg ctagccatcg gcagattaaa aaacacatgg
     8161 gccagcaaaa tgccttgcag gccgtagggc gaaaaggtcc actccagacc aagtgattgc
     8221 cagagcgagg ccagccagcc ctggcgacca tagacgctaa gaatgccgaa aacggcgacc
     8281 agcaccggga ggatcaaggt cattgcgcac agacgcaaca gcacctgtcg cccaggaaag
     8341 cgcctgcgat agagcgcgcg ggcgaggaat attgcgggta cgacagagag cagcgccgag
     8401 agaaacgcct gccagaagga gaagcgcacc acatgccaca gatagctgtc ctgccagact
     8461 gccacccagt caccctgcgg cgcgttccac cacagggcga gaaacgccgc cagcgcaacc
     8521 gctaccacca gtgtggcggc gcttattcct ggaattaacc agccgggaat taacggctga
     8581 cggcgcgttg ccattcgcta atccatgcct gacgttgtgc cgccacttcg gcgggcgtga
     8641 actctagcgt ggtggctggt ttggtcaatt gttcaaatcc tgcaggcaga gtgacgtttg
     8701 ctaccggata catccagttg ccggttggga tcgcattctg aaaagccgga gaaaccataa
     8761 actggaggaa tttttgcgcc agctctggct gcttgctggc agcggtgcgg gcggcaactt
     8821 ccacctgcag atagtgacct tcgctgaagt tcgcggcggc gtagttatct ttcttctctt
     8881 cgagaatgtg ataagccgga gaggtggtgt aactcagtac cagatcgctt tcgcctttta
     8941 aaaacaggcc gtaggcttcg ctccagcctt tagtgaccgt gacagttttc ttcgccagtt
     9001 tctgccaggc ttgcggggcg ttatcgccat agactttttg catccatagc aacagaccca
     9061 gacctggtgt actggtacgc ggatcttcat aaatcacccg ccagttttga tcgctctcaa
     9121 ccagttcttt caggctttgc ggcggatttt tcagtttgtt cttgtcatag acgaaggcga
     9181 aatagccata gtcaaacggc acaaaggtgt catttttcca gccgccggga acgtttacag
     9241 cctctgtcgc gacgccgctt ttggcaaaca acccggtttt gctggcggcg tccagcaggt
     9301 tgttatccag ccccagcacc acatcggctt tgctgttttt gccttccatc cgtagacggt
     9361 tgagcagcga aacgccatct tccagcgcca ccagtttcag ttcgcaatta cagtctgctt
     9421 caaaggcttt tttgactacc ggaccaggtc cccagtcagc ggcaaaggaa tcgtaggtat
     9481 aaacagtcag aacaggttta gcgaaaacag gcgctgtgca cagcaacagc aggggaagac
     9541 atttttttaa cactttgcac ctcacaaaag agtggcaaag gacttgagaa ggagcctcaa
     9601 atcccttcgc cggcgttatc cggatcaggt tcgacgggta ttttctcagc gcacgcggct
     9661 gcgtggcacc ccgttgagaa cggcgttagt gtagtgattt tgttatcaac cagcaatcat
     9721 ggatccggtg gtgcaaacca cgctgattta aaatcgaacc agccgagggt gttcatgcgc
     9781 aggccgcgca tactgcgttg cccctgaatg atcagccagt ggtggataag tggcaccatt
     9841 gctttgctgg cgaccagttg ctggcaccag ttcgccaggt tcatttcgcc attgcgccag
     9901 cgggcggcgt cggcttgcca gtcgatcgga atacagtgtt ggagtagcgg cacctcgcac
     9961 aagtgcgcga acagcgaaaa atccagcggc agcgtaaagt tggcgctgtt aagccagata
    10021 tcgctctcga tctctccttc atgccactga tcgtagctga tctctttgat ttccagcgtg
    10081 acctggtgac tcgccagaat ctgctgcatg atcccggcaa tcactcgatg ttcaatatga
    10141 tcctgataaa aggtcagggt gagatgttcc agcccggcgg gtttttcgca atgtgtcggg
    10201 cgggcatggt gccagcgggg gagcagtcca taagccggga accagagttg ctggtattgt
    10261 tcctcggcga aatagaccag attcgtcgga gaaagcacat agcttaccca gtccctgact
    10321 tgctgattcg ccccgcggtg ggtgcggttg tcgaacagta aataatagca accttcctcc
    10381 aggcggcttt caatctcttg ttcctcgccc tgcggccctt tcagcatcag ccctccggct
    10441 ggctcgtcgg caatttccgt tagaacccag acgttaactt cgtcgattaa tgcccggtaa
    10501 ccgaagaagt catcgaatgc ctgaattttc agttgattgg tgctgttgcg gatcaccgca
    10561 tacggaccgg tgccgatggg atggctggca aagttactga gggtttccca ttcgcgcggc
    10621 aggatcattg ccggaacttg ccccagcagt aacggcaacc agcggtccgg ttgcgtgaga
    10681 tggatatcca gcgtccaggg cgtcggcgac acaatgtcag caatatgcga atagagcgga
    10741 agcgtattga ttcgttttaa agaggcgatc acatcgtcca tttccagttc acgaccatgg
    10801 tgaaaatgga ctcctggacg caaaaagaaa cgccagtgaa gcggtgatat ttgctgccag
    10861 tggtgtgcga tgtctgcttc cagttcccca ttttcctcat ttatgcgcgt tagcgaactg
    10921 aagatttgcc gggcgatatg ggtttcagaa cggcgcaatg cgctgccagg tagcagatta
    10981 cgcaacggac gatagtagag cacgcgcagg atgtgccgcc cctggcggaa gctgcggccc
    11041 agatgagaaa ccagcatttg ccgcacagtc gctttgtcgc caaccaactg cactagttgg
    11101 tcgatacgat cctgctccag caggtcttcc gctcgctgtt gctgaagcgc cagcccggta
    11161 tagaggaatg tcagacgcga gcgtttaccg cgcccgactt ccgcttccca cgtcagccag
    11221 ccgcgatcct gcatggtgtt gagcagggtg cgcatatgac gacgcgagca actcagtaac
    11281 gctgccagtt cgttgagcgt ggtctcctga gatttaccct cgcagcattg ccacaggcgg
    11341 atgaactgtt gttgcagacg agcagatggc ataaaagggg aactcctgcg caaaagacag
    11401 caattttatt ttccctatat taagtcaata attcctaacg atgaagcaag gaggtgcccc
    11461 atgcgtcagt tttatcagca ctattttacc gcgacagcga agttgtgctg gttgcgttgg
    11521 ttaagcgtcc cacaacgatt aaccatgctt gaagaactga tgcaatggga ggggagtcat
    11581 tctgactact gatttgcgga catcatgtgt gactgagtat tggtgttaat cgccacgcca
    11641 gcagtgatta tctgctggct tttttctttt ggcgattgag tgccggacgg tgtacaaact
    11701 aacgcataaa cgccggttgc ttctcttcat aagacgcaat agcatcgtcg tgctgcagag
    11761 tcaggccgat gctgtccaga ccgttcatca tgcagtggcg gcggaaggca tcgatggtaa
    11821 agcgataagt tttctctccc gctttcacct cttgcgcttc caggtctaca tcaaaatgaa
    11881 tccccggatt ggctttcacc agcgcaaaca gctcatccac ttctgcatcg cttaatttca
    11941 ccggcagcag ctggttgtta aagctattgc cgtagaagat gtcagcaaaa ctcggcgcaa
    12001 tcaccacctt aaaaccgtaa tcggtcaatg cccacggcgc gtgctcacgc gaggaaccgc
    12061 agccgaagtt ttctcgtgcc agcaaaatgg aagcgccctg atactgcggg aagttcagca
    12121 cgaagtccgg gtttggctgt tggccttttt catccagaaa acgccagtcg ttaaacagat
    12181 gcgcgccaaa acccgtacgg gtcactttct gcaaaaactg tttcgggatg attgcatcgg
    12241 tatcgacatt ggcggcatcc aacggaacca ccaggcctgt gtgtttgata aatttctctg
    12301 ccatggtgtg ctccttattt aatgttacga atgtcggcga aatgtccggt cacagccgcg
    12361 gcggcagcca ttgccgggct gaccagatgc gtgcgcccgc cgcgcccctg gcggccttca
    12421 aagttacggt tgctggtgga ggcgcaacgt tcgcccggat tcagacggtc gttattcatc
    12481 gccagacaca ttgagcaacc tggcaagcgc cattcaaaac cagcttcgat aaaaattttg
    12541 tccagacctt ccgcttccgc ctgggcttta accggaccgg agccgggaac caccagcgct
    12601 tgtacgcctg gcgcgacttt ccggcctttg gcgatttccg ctgccgcgcg taaatcttca
    12661 atacgcgagt tagtacagga accaataaac actttgtcga tagccacttc tgtcagcgga
    12721 atacccggtt tcagccccat ataggccagc gctttttctg ccgacgcgcg ttcaaccgga
    12781 tcggcaaacg aagccggatc gggaatattg tcgttcacag aaatcacctg gcctggattg
    12841 gtgccccagg tgacctgcgg cgaaatctct tctgcttgca gagtgacaac ggtatcgaaa
    12901 gttgctcctt cgtcggtttg cagggttttc cagtaggcaa cggcgtcgtc gaaatcttta
    12961 cctttcggcg catgcagacg gcctttaaca tagttaaagg tggtttcgtc cggcgcaacc
    13021 agaccggctt ttgcgcccat ttcgattgcc atattgcaca gggtcatacg accttccatg
    13081 cttaaatcac ggattgcttc gccgcaaaat tccaccacat gcccggtacc gcctgcgctg
    13141 ccggttttac caataattgc cagcacgata tcttttgcgg taatgcccgg cgcggctttg
    13201 ccctggactt caattttcat ggtcttcgcc cggccctgtt tcagggtttg cgttgccagt
    13261 acgtgttcaa cttcggaagt gccgatacca aaggccagtg cgccaaacgc gccgtgggtg
    13321 gcggtatgtg agtcgccgca gacaatggtc atccccggca aggtcacacc ctgttccggc
    13381 cccattacgt ggacgatccc ctgatacggg tgattcaggt catacagctc gacgccaaat
    13441 tctttgcagt ttttgatcag ctcctgcatc tggatgcgcg ccatttcacc gcaggcatta
    13501 atgtctttcg tctgggtaga gacgttgtga tccatggtgg cgaaggtttt gcccggctga
    13561 cgtaccgggc gaccgtgggc gcgcagacca tcgaacgcct gcggtgaggt cacttcatgc
    13621 accaggtggc ggtcgatata taacagcggg gtttcgtttt cggcttcgta cactacgtga
    13681 gcgtcgaata atttttcgta taacgtctta gccatgatta caccccttct gccacatagc
    13741 gggcaatgat atcgcccatt tcatcggtac taacggcggc agcgccacgg gctaaatccc
    13801 cggtgcgaat gccttcttct aatgcgcggt taatggcgcg ttcaatggcg gaagccgcat
    13861 catcggcatc caggctgtaa cgcagcagca gcgccagcga cagaatttgt gcaatcgggt
    13921 tggcgatatt tttacctgcg atatcgggtg ccgagccgcc cgccggttca tacagaccaa
    13981 aaccttgctc gttcaggctg gcggaaggca acatccccat tgagccagtg atcattgcgc
    14041 actcatcaga cagaatgtcg ccaaacaggt tggagcacag caagacgtca aactgtgatg
    14101 gatctttaat cagctgcatg gtggcgttgt cgatgtacat atgcgccagt tcgacgtccg
    14161 ggtattccgt ggcgatctcg ttaacgatct cccgccataa aatagaggat tgcagcacgt
    14221 tggctttgtc gattgaggtg actttgtggc gacgcttgcg ggcagattca aatgcgatgc
    14281 gggcaatacg ctcgatctca aaacggtggt acacctcggt atcaaacgct ttttcatatt
    14341 gtccgctacc ttcgcggcct tttggctgac cgaaatagat gccgccggtc agttcgcgca
    14401 cacacaggat gtcaaagccg tttgcggcaa tgtcagcacg cagcggacag aatgcttcca
    14461 gcccctgata cagttttgcc ggacgcagat tgctgaataa tttgaagtgc ttacgcaaag
    14521 gcagcagtgc gccgcgttct ggttgctggt ctggcggcaa atgttcccac ttcggaccac
    14581 ctaccgagcc aaacagcacg gcatcggctt gctcacaacc ttcaaccgtc gcaggcggca
    14641 gcggctgccc gtgattatca atggctgcgc cgcctacatc gtaatggctg gtggtgatac
    14701 gcatcgcaaa tcggttgcgc acggcatcca gcactttcag cgcctgggtc atcacttccg
    14761 gaccaatacc gtcccccggc aatacggcaa tatggtaatt cttcgacatc acacggtttc
    14821 cttgttgttt tcgttgtgtt gagctttgcg ttgcaactct ttttcgactt ctgcggcacg
    14881 ccagatattg ttcagtacgt gcaccatggc tttggcggag gactcgacaa tatcggtggc
    14941 caggccgacg ccgtggaagc ggcgaccgtt gtagttggcg acaatatcca cctgacccag
    15001 cgcatcttta ccgtgacctt tggcggtcag gctgtatttc accagttcga cgttatagtc
    15061 agtgatgcgg tttatcgcct ggtagacggc atcaaccggg ccgttgccgt tagcggcttc
    15121 tgctttaact tcttcgccac aggccagttt gacggcggca gtggcaatat cgttagaacc
    15181 tgactgcacg ctgaagtaat ccagacggaa atgctccggc tcttcttgct gcttatcgat
    15241 gaaggccagc gcctccagat cgtaatcaaa cacctggcct tttttgtccg ccagcttcag
    15301 gaaagcgtcg tacaggttgt ctaaattata ttcactttct ttatatccca tctcatccat
    15361 gcgatgtttc accgccgcac gccccgaacg agaggtcaga ttcagctgga tttggttcag
    15421 accaatagat tctggtgtca tgatttcgta gttttcgcgg tttttcagta caccatcctg
    15481 gtggataccg gaggagtgtg cgaatgcgcc gctgccaaca atggctttgt ttgccgggat
    15541 cggcatatta caaatctggc taactaactg gctggtgcgc catatctcct ggtgattgat
    15601 ggcggtgtgg acgttgagaa tatccttacg aactttgatc gccatgatga cttcttcaag
    15661 ggaacagtta ccggcacgct cgccgatccc gttcattgcg ccttctacct gccgtgctcc
    15721 ggcatgtacc gccgccagtg agttgccgac cgccaggccc aaatcgtcgt gggtatgtac
    15781 ggaaataatg gctttgtcga tgttgggtac gcgttcatac aggccgctga tgattccggc
    15841 gaactcaaat ggcatggtgt agcccacggt gtccggaatg ttgatggtgg tggcaccggc
    15901 attaatcgcc gcttcgacca ctcgcgccag atcggcaatt ggggtgcgtc cggcatcttc
    15961 gcaagaaaat tcaacatcat cggtgtaatt acgggcgcgt ttcaccatat agatagcgcg
    16021 ttcgatcact tcgtccagcg tgctgcgcag cttggtggcg atgtgcattg gcgaagtggc
    16081 aataaaggta tgaatacgga aggcttcggc gactttcagg gattcggctg ccacgtcgat
    16141 atctttctcc acgcagcgag ctaacgcgca tacacggctg tttttaacct ggcgagcgat
    16201 ggtctgcact gattcaaaat cacccggcga agagacgggg aaaccgactt ccatcacgtc
    16261 aacacccata cgctcaaggg ccagcgcaat ttgcagtttt tctttcacac tcaagcttgc
    16321 ctgtaacgcc tgttcaccgt cgcgcaatgt ggtatcgaaa ataatgactt gctggctcat
    16381 ggtttgggtc cttgtctctt tttgagcgcc tcgcttcggg cataaaaaaa cccgcgcaat
    16441 ggcgcgggtt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
    16501 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
    16561 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
    16621 accctgaagc aataaaaacg tcacgccatt gtgagttcgg catccgtaaa attagctaat
    16681 tgtgctgcgg tggttaaaac aagcgatatt aatttctgct taaccgccga tgcttttcat
    16741 cggttgacat atttcagaat aattttttgc atctaatcaa cgagggaaag ggggataaaa
    16801 tgcacgcgtt acaaaaccta tcctgatgat ttgtattgaa ttatatgttt
//
LOCUS       DAPQZD010000102        16785 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPQZD010000102 DAPQZD010000000
VERSION     DAPQZD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.102"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(259..795)
                     /gene="yghD"
                     /locus_tag="RO454_003210"
     CDS             complement(259..795)
                     /gene="yghD"
                     /locus_tag="RO454_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDY0935004.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAA
                     VFLFCAVYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIIAARKSGTTTQAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVLRLEFGRG"
     gene            complement(797..1975)
                     /gene="gspL"
                     /locus_tag="RO454_003211"
     CDS             complement(797..1975)
                     /gene="gspL"
                     /locus_tag="RO454_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDY0935005.1"
                     /translation="MSSILEIFFPLCDSDTVRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPFSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1972..2949)
                     /gene="gspK"
                     /locus_tag="RO454_003212"
     CDS             complement(1972..2949)
                     /gene="gspK"
                     /locus_tag="RO454_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDY0935006.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHQAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2946..3551)
                     /gene="gspJ"
                     /locus_tag="RO454_003213"
     CDS             complement(2946..3551)
                     /gene="gspJ"
                     /locus_tag="RO454_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDY0935007.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMALQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3548..3919)
                     /gene="gspI"
                     /locus_tag="RO454_003214"
     CDS             complement(3548..3919)
                     /gene="gspI"
                     /locus_tag="RO454_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDY0935008.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3916..4479)
                     /gene="gspH"
                     /locus_tag="RO454_003215"
     CDS             complement(3916..4479)
                     /gene="gspH"
                     /locus_tag="RO454_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDY0935009.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(4483..4938)
                     /gene="gspG"
                     /locus_tag="RO454_003216"
     CDS             complement(4483..4938)
                     /gene="gspG"
                     /locus_tag="RO454_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDY0935010.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4955..6178)
                     /gene="gspF"
                     /locus_tag="RO454_003217"
     CDS             complement(4955..6178)
                     /gene="gspF"
                     /locus_tag="RO454_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDY0935011.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQHLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6178..7671)
                     /gene="gspE"
                     /locus_tag="RO454_003218"
     CDS             complement(6178..7671)
                     /gene="gspE"
                     /locus_tag="RO454_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDY0935012.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7671..9731)
                     /gene="gspD"
                     /locus_tag="RO454_003219"
     CDS             complement(7671..9731)
                     /gene="gspD"
                     /locus_tag="RO454_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDY0935013.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLALVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9761..10720)
                     /gene="gspC"
                     /locus_tag="RO454_003220"
     CDS             complement(9761..10720)
                     /gene="gspC"
                     /locus_tag="RO454_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDY0935014.1"
                     /translation="MARVVFRDARIYLIQLLTKIRHTLSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTKAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10738..11148)
                     /gene="gspS2"
                     /locus_tag="RO454_003221"
     CDS             complement(10738..11148)
                     /gene="gspS2"
                     /locus_tag="RO454_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDY0935015.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11214..12023)
                     /gene="pppA"
                     /locus_tag="RO454_003222"
     CDS             complement(11214..12023)
                     /gene="pppA"
                     /locus_tag="RO454_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDY0935016.1"
                     /translation="MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12153..16709)
                     /gene="sslE"
                     /locus_tag="RO454_003223"
     CDS             complement(12153..16709)
                     /gene="sslE"
                     /locus_tag="RO454_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDY0935017.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTG
                     ATCNGESSDGFTFTPGEDVTCVAGNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNGEA
                     LSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGCNSQRWFSLTARNVNEGQIQGV
                     INKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPH
                     YNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASMTV
                     GTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYV
                     TQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTG
                     EVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIFE
                     KFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNVSVWLWNKIEYRYENDKDDELGF
                     KTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESSASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVTFK
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNFTGGVAEFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKQWYPEGDLPEFYSDREGMKGWNLFQLMHRKARGDDVGKTKFGERNYCAES
                     NGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMNFEGGVSQSAYETLAA
                     LNLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         3711 a         4787 c         4218 g         4069 t
ORIGIN      
        1 tactcaatga tttattacaa ctattaataa tcataatgtg tcaaaacact taagcatctt
       61 tacagtctta gaaaggtgtc gattaacgaa ggctacatgg tgaaaaacag gttccttcag
      121 tgttaccgaa cattccgtgg tggtctggag ggagataaat cttgctacat cggaactcag
      181 cccggtgagc tggtttgaca gttccgcact caccattggg ctaagggtta tcaggtgggg
      241 ttaaggaaat ggcaatacct acccccgtcc aaactccagt cgcagcacat ttaccatccc
      301 tggcttctca gcagcactga catcaatttg tgtcacccgc agcgcatatt tttcatccag
      361 tgcgtttaac catttcagaa ggtcattaaa caccacaggt tctatccaga cctgaatatt
      421 ctccccacgc tcggctatcc gcttgatgac caccgagtgc gcggaagcgc tgtcactgat
      481 gacccgcgat acctgtgctt gcgttgtcgt gccggatttt cgcgccgcaa taatatccgg
      541 cgcagtgctc ttcagtcgcg cgttcatcgc caccagctgc tgcaacatcg tctcctgttg
      601 ttctatccgc tcgctcaacg gctgccagat gaggacgtaa tatacggcgc aaaacaggaa
      661 cacggccgcg gccagtaaca tgcctttttc acgcggcgaa cgccccgcca ggtgctgtgc
      721 cagccagtgt tcgccacggc ttaactggcg ttcacgccat tgctgaaaat agtgaataaa
      781 tttatcgcgt aacatgttat ttcctccgca acgttacgcc gccggaaacc gcatcaccct
      841 ctttctgtaa cgcgtcctgt tgcacaacat aatctgccgc cagtgcgcta cgcagtttat
      901 cgaagctggc aaagttcgca gcccgtagct ggaggtgaag cgtctggcgt ttttgatcaa
      961 aggtgaaacc acgcatttcg atgtcggtaa gtgacgctga tttcagggta ctggcgatcg
     1021 ctgacaattc tgcgagcagc cgggtatcgt cggcctgtgg gcgatatttt ttcagcgcca
     1081 tcgtcacctg agagcgtaaa ttcacaatcc gcttctgctc cgggaatagc gttaagaact
     1141 gtttctccgc ctgggtgcgg ctttgcgcca cctgttcgct gacgctccat aacgtcacgc
     1201 cccgttccac tgccagcgca accagaatca gcaatatcgg cagaatcatc acccgccagc
     1261 gcgcccactg ttttcggtag ctgacgcgag gctgccacgg tccagtcagc aggttccctt
     1321 ccggttcgcc ataagtggta atggcgggca gagccgtcac tgtcaggcgt tcggcgtctg
     1381 caccagccca tgcagacagt tcttccggtg caatgccgac cacggttagt gaaaacggta
     1441 aatcctgctc attgagctgt gcgcggaaca tgaccggagc cagcgcccgc ccggcgctcc
     1501 atccccggca ttcatcgatg cggcagataa cccgttgcgc atcgccagcc atatagccac
     1561 aaggaatgga catccagtct ggcgcgacgg tagcgcgggt gatgccgttt gcctgcaacc
     1621 attgcgcaat gttacgcata tgctgttggt gaatcactgc tacggttgcc agttgctggt
     1681 cgattttcaa cggggcgaaa tgcagttcat cgatatcctg attcagctct tcttccagca
     1741 aggcgggcag aatagtcggt atctgcttgc gggcgacatc aggcagttca acctgccaga
     1801 cgctgatcca ttcgccggga atgtagagtc gaatcgcatc ggtttgcagc cattgctgga
     1861 gacgttcgtc agcaacgtca ggccagatac cgtgttccac atcgggtgta cggcgctgcc
     1921 agcggacggt gtcggagtcg caaagcggga aaaaaatctc aaggatggaa ctcactcact
     1981 ttctcctgtc tgatgccaga gaacagaaaa gtgttgtggg cccatgcgga caattaacga
     2041 gttcatcgtc agttcaatct cattcacggt gatatctgaa cgcagccaga agtaattgct
     2101 gtccacgctc aggacggttt ttagctgttt tttagtacgc tcatcgacgt ctgcgagtag
     2161 cggctgtgca agaaactgat cgacatcttc ccagcccttc gccggacgtt gttgtaataa
     2221 cgcccgcgcc tgtacagggc ttaaccacgg gtcaaacagc gcctcaagaa tcacactttg
     2281 cgtgacatcc agggtgttga tgttgatttg ctggcgggtc atcggcagcg cacagaccag
     2341 cggtttcagt ttttgataaa gtccggcgtc cattccctgc accacgcgca tctcactgat
     2401 atcggccagc ggttgattag cggcgtaaaa cggcaccgaa cgggcgagat actcgctgtc
     2461 ttcacgaccc agacgcgtct gcacgctgcg gtcttcgtca ataaactccc acaggctttc
     2521 ggctatcagt tcggcacgat aagcaggcac atcgaggcgc gagatcaggg caatcagttg
     2581 ttgtaccgcg agcggacgcg acgccgtcgt cggctgagcg agggcattca ggttaaagca
     2641 ggcctgtgcg tcacgcagag tgacggcgat ttgcccctgc ggcagtggga aaaaacgagg
     2701 gccagaagcc cacggctgtg cctgatgcac acgcttttca tttttcaggc tcagactgag
     2761 tgcgctcaac gccaggcttt ccgcactggc gctgtaccac agcgcctgct ggtactcctg
     2821 ctggctgcgc gttcgcccaa gttgttgctg catccgcccg gaaagcgtga tggtcaccag
     2881 catcataacc gccagcaata ccagcaccac gaccagtgcc attccgcgtt ttggtggtga
     2941 ggtgatcatg ataattgcgg cccgcgtaac aaccagatgc gttcaatttc gccccattgc
     3001 ggcgaatgca gggttatgcg tactgccaca ggaatcgcct gcactgatga ccagctctcc
     3061 tgccagcgcg tgccgtcgta gaactgcaaa cggagcgaat ccgccgggat taatttttgc
     3121 gttgttggct tcacgctgcc tgccgcatcg gtcagtggcc aggctaaccg ttcgagataa
     3181 ccaccatgaa tgcggtaacc gacggtgagc agattactgc gcggcagacg catcaacgga
     3241 ttaaccacgc cgccgcgcac aaaacgcatc ccttcactct cagacgccag cacgccagcg
     3301 cccgccagta acgctggttc acgctggccc tgatcgcctc ttaccggacg cggcatcatt
     3361 tgtgtcagat cgtggttcaa aaaactcatc gtttgctgca tgaggtttag tttttgatcg
     3421 tgtccggcga cggcgctatt cacgcgtgta acgccgtttg tcacctgcag cgccatcagt
     3481 gccagtgagg caaaaatggc aattgccacc agcatttcca gtaacgtgaa accagcgcaa
     3541 gtccttctca ctgttggcct cccacggcgc taaaccaggc gcgtcgtgac tgaatcaccg
     3601 acgaaaagtc ttcgtgacgg ctgacttcaa tatccactgc ctggagcagc gcgttgcctg
     3661 tactcagcgg tgtggttcgc cagtaccagc ggcttcctgc catatcactc tcgccctggt
     3721 gcctgaccgc tcgttgttca cgggtcatca ggtaaagcag tgcggtctgg ttttcagcta
     3781 cccagcccgc taccgttttt tcttcaagga tttgctgatt actcagcgcg ccgctggcga
     3841 tctgtaacac cgccatggcg gccaacgcaa aaatcgccag cgcgagcatt acttccagca
     3901 aggtaaatcc acgcttcatg gcatcccctc atcacattga ttgagggata acacgccatc
     3961 gtgcgccagc tttaccgccc aacacgcgtt ttgtgccgac gcgtaaaaac gcagtgtaaa
     4021 cggcgtggcg ggttcaaaag gcgaaaaacg gatctgcggt gtcttctttt tcgcctcttt
     4081 ttgcagctcc agttcaatat cgtgcagcgt caggcgacga cgctgcagct ccagcgcata
     4141 ttccttctgc cagatatcac ttccgggttg taacagcatc tgcacctgtt tcggcaccgt
     4201 aacctgtgcc gataaccggg tcgcagaaac gggcaaccat tgcccgtgac gacgctgcat
     4261 aaactggtaa cctggcgggt cgattagcac accgagtgtt tgcccttcga tcactgccct
     4321 gtccttaaac tgcgcaaagc gagtcagaaa atcctgcgcc gcttttttcg caggcggctc
     4381 tgaagcggtc gcaaacgtct gtaccacgcc cgcactggca aggccgataa ggaaaatcac
     4441 cagcatgatt tccagaagtg tgaatccgcg ttcaggcact gattactgaa actcctgcaa
     4501 attccagtta ccgatatctg cgccagcgcc ctccccgttt tcctgaccat ctgctcccag
     4561 ggtataaaca tcaaacagcc ctttttcacc cgggctgaga tactgataat cattgcccca
     4621 cggatccttt ggcagtcgtt taatgtatcc accggtacgg tagttacggg aatccgccat
     4681 attggccggt tgctggatca gcgcctccag cccctgttcg gtagtcggat aacgcccgtt
     4741 atccagtcgg tacatatcca gcgcattctc cagcgccacg atatcgctga tggctttttg
     4801 ccgatcggct ttctctttgt tgcccaacag gttaggcacc accagacttg ctaggacgcc
     4861 aagaataaca atcaccacca tcacttccag cagggtaaaa cctgcccgtg gtttttgtgt
     4921 gcgggataac gaattcatgt gataactccg taaattacat tccaaccata ttgttcagtt
     4981 gcagcatcgg ttcgaggatg gcgatgacga taaacagcac cacgcccgcc atcatcacca
     5041 ccagcgccgg ctcaaacagc cctaacgcca gccccacctg ggtatcaaat tcccgttcct
     5101 ggttgaccgc ggcctgttca agcatggttt ccagttcacc gctctgttcg ccggaggcga
     5161 tcatgtacag catcatcggc gggaacaggc gcaactccgc cagcgcggcg cgcaggctgc
     5221 ttccttcgcg gacgcgatct gccgccagca gcagttgttg ctcgacataa cgatttgccg
     5281 acacggcggc ggcggtctga atgccttcca gcagcggaac actgctggcg gtgaggatgc
     5341 ttaatgtgcg ggaaaaacgc gccgtattca gtccgcgcgc aacacgcccc gtcacgggca
     5401 aacgcagcaa ggttttatcc cagcgcaggc gcatcgcagg atttttgagt agccgttgcc
     5461 ccagcaccag aagccccagc aaacccgcca gccagtacac gccgctggtc tgtaacgtgt
     5521 cgctcatggc gatgagcatt cgggtggagg caggcagcgc gtgtccgaga tgatcaaact
     5581 gttcgataat tttcggcact actgccgtca gcaaaatagt gaccacgccc gttgccacca
     5641 ccagcagtac cagcgggtag agcatggcct gcagcaggcg tgatttcaga tgctgccgct
     5701 gttcggtgta atccgccagg cgattgagca ccacgtcgag atgtccggat ttttctccgg
     5761 cagcgaccat cgaacaaaac agggagtcaa agacgcgggg atgttcgcgc aggctgtccg
     5821 acagggtata accttcctga atccggctgc gcagcgccat tccgaggctt tttacatgca
     5881 gtttttcact ttgctcactg accgcctgta agcaggtttc cagcggcatt gctgcctgca
     5941 ccagcgttgc cagttggcgt gtgaacagcg ccaaatctgc cgccgccacg cgacgatgtg
     6001 catgccgccg acgctgcaac attccccctg tcgaagcatt cagccgggct tcaatgtgca
     6061 cggggataag ctctttacca cgcaacaatt gacgggcatg acgcgcggaa tccgcctcaa
     6121 tcattccttt ggttttgcga ccattacgct ccagcgcctg atagtaaaac agtgccatta
     6181 cgcctccatg gttacccgca gaacttcatc gagagaggtt tctccggcga gcactttctc
     6241 aatgccgttg ctgcggatac ccgcagagtg ttgccggaca taacgttcca gctccagttc
     6301 cccggcctga cggtggatca aatcacgtaa tgtggcgtcc accacgatca gctcatggat
     6361 ggcagtacgt ccgcgaaaac ctttgtgatt acaggcggga cagccctgcg gatggtacag
     6421 cgtgacggtt cgcgcatcgg taatccccag caggcgtttt tcttcgtcgg tggcaggcgc
     6481 ggactggcgg caatcgggac acagcgtgcg aaccagtcgc tgcgccatca cgcccgtcag
     6541 actggaagag agcaggaaag gctcaacgcc catatcctgc aaacgcgtga tcgcccccac
     6601 cgccgtgttg gtatgcagcg tggaaagtac caggtgtccg gtcagtgaag cctgaacagc
     6661 gatttctgcg gtttcggtat cgcggatttc accgaccatc accacatccg ggtcctgacg
     6721 caaaatcgcg cgcaggccac gggcgaaggt catgccgacg cgggtgttaa cctgcgtctg
     6781 accgatccct tcaatcatgt attcgatagg gtcttcaacc gtgagaatgt tacgcgagtg
     6841 gttgttcagc tcctgcaatc cagcgtacag cgtggtgctt ttgccggaac cggtcggccc
     6901 cgtcaccaga aaaatgccgt gcggtttgtg taacagctgg cgcaactgcg cagtcagttc
     6961 gagacttaaa cccagacgct ccagcgtcag gcgagcctgg tttttgtcca gcagtcgcag
     7021 caccacccgt tccccccagg cggaaggcat ggtggagaca cgcacgtcaa tcgcccgacc
     7081 gcccagcagc agcgcaatgc gcccatcctg cggtacgcgt ttttcggcga tatccagccg
     7141 tgccatcacc ttaatacgcg ataccagcag cgaggccagt ttgcgccccg gacgcagcat
     7201 ttcatgtagt gtgccgtcaa cacgaaaacg gatcaccaga ctcttttcaa acgtctcgat
     7261 gtggatatcc gaagcgcctt ctttaatcgc ctctgccaac atggcgttga tcagtttgat
     7321 gattggcgca tcgtcgtcac tttccagcag atcttccgtt tccggtagtt cttctgcgag
     7381 agtaaaaaag tcctcagcag aaccgagatc ttccatcagc tggcgggcct cggaagagtc
     7441 ccgctgccag accgcattca gccgctgttc aaattcggcc tcgtcgattt gcctcagcgt
     7501 aaaagacgcg ttcagccccc gctgcagctc ctgcaaaaca gagagcgaca acggatgaac
     7561 atggaggatc tccagcgacg cttcgcacca tgccaccagg ctaaaccgac ggctgaaact
     7621 gtagggcaga cgcacggtgt tagcggtggt ttcctgtgct acaggcacca ttaacgcgtt
     7681 ctcccggcat taaggaacgc gcgaacttct ggcggtagag cctgattttg cgctggcagt
     7741 accggctgcg cggtgtgcgg catcaggctc aagccttgct catcgcgata gatctgttcg
     7801 gcgcgcatat agttatattt gcgctgcgac acgccgtctg ccgccatacc gtcacgcaga
     7861 atggtcgggc ggataaatac catcaggtta cgtttttctt ttttatccgc cgtcgattta
     7921 aacaggttac caatcaatgg gatatcgcca agcagcggca ctttcgccac gctttctccc
     7981 gcctgatcgt ccatcagacc gccaagcacg atcagctcac catcgtttgc cagcacggtg
     8041 gttttcagtt tgcgctcgcc aaacacgacg tcgagactgg tctgtccttc caccttcgac
     8101 acttcctgct caatcaccat ctgtaccgcg tttccttcgt taatctgcgg cgtgactttc
     8161 agcatgatgc cgactttttt cctctctacc gtattgaaag gattgctgtt attagagcca
     8221 acagtagagc cggttaatac cggaacgtcc tggcccacca tgaagaaggc ttcctggttg
     8281 tccagcgtgg tgatactcgg cgtggagagc acgttcgagc tggagtcgtt tttgaccgcc
     8341 tgtaccagcg ccatccagtc gcctttcacc acgccaaccg ccgtaccgct aaagccagaa
     8401 agaagctgag cgagcgtgga gagatcgccg ttagtatccg ggttaatggt ggtagcgccg
     8461 ttttcactga tcaccgtgga gcctttctgc ggttttgcct gagaaatggc tgcacccagc
     8521 gtgccaatag ggatctgcgt accgttagca aactgcatta atccggcatc tttcgacgcc
     8581 cactgcacgc cgaaattgat attgctacct tcggcaactt ccacgatcaa cgcctcgacg
     8641 tgcacctgag cacggcgaat atccagttgt tcaatcacgc tttgcagcga ctgcatgatg
     8701 tcctgcggcg cggtaacaat cagggcatta ctgtgtttgc tggcggcgat ggagacaacc
     8761 tcacgcccgc taccgacggt gccttccgcc tcttctttag ccgccgtgag cgtgccgctg
     8821 acctgcttca gtacatcgac cagatcttcg gctttgctgt atttgagata gaaaacctgg
     8881 ctgttgccgc tgcgttccat ttctgagtcc agccgacgga tcaggcggcg cattttgtcc
     8941 cgcgtggccg ggtcaccact gacaatcaca ctgttggtgc gttcgtcggc gacaatttga
     9001 gatttcagcg tcgcaggctg gttctcgccg ctgtttttcg tcaggctttc cagcacgcgg
     9061 gcgatttccg aagccgaggc gttatccagc gggatcacct cttcggtgcg attacccgca
     9121 tgatccacgc gctggatcac ttccgtcagg cgctccacaa ctgaggcgcg tccggtaagc
     9181 ataatcacgt tggagggatc gtaattaaca acgttgcctg agcctgcgct gtcgatcatc
     9241 tggcgcagaa tcggtgccag ctcgcgtacc gacacattac gtaccggcac gactttggtg
     9301 accatttcat cgcccgcgta gttgtcgctg ccttcaccaa ccagcggcag cggctcgact
     9361 ttcgcggcac tggatttcac caccttcagc acgtcgtttt ccatcggtac gacggcatac
     9421 ccctgtgctt ccagcaggtt aaggaatagc tggtaatact ggcgttcatt gagcggggtc
     9481 atggtgcgaa tactcacttt cccctgaacg cccggcccca taatgatggt tttattaagg
     9541 ttagcgccga cggtttcgat gaacgatttc aggtcggtat ctttaaaatt agcggtgaaa
     9601 gtggcttctt ccgcccagac cggtgaactg cataatgccg ctgccagcac cagcgccagt
     9661 aaacgctttt ttgttttgag gccagttgtc ttcttacgcc agaccgacaa cgtcatatca
     9721 cgccaaaaca cgatgaatga ttctcctgga ttaaatgcgg ttagcgcagc gcgatggaaa
     9781 tgtcgtagcg cgcacccttg cgtaaaaccg taagttgaat ggaatccatt gaaggtaact
     9841 gccgcatcag agcaatcatt gctcgtggat cagtgaaatc ctgctgattt agcgcaatgg
     9901 cgatatcgcc ttccttaaaa ccgctggcat cgaacagaga acgatctgcc cccggtttca
     9961 ctgcataacc gacaatccct tccttacgca caggcgtaag ctggatatag ttaaaaattt
    10021 tctgcggatc tttcgccagt gcctgacgca cggcagccgg gatctcaact ggcgcactgg
    10081 cagcaggctc agcaacagct tgctttgctt cgtcgctgac ggcttttttg ctggtcacgg
    10141 ctttggtggg acgcttctct tctgccagac tcagacgttc cattttcccc tgatagcgca
    10201 gcatcacatg gtcacgatta atttcctcaa tcaccgcgtt gtgagagcca agccgttcgc
    10261 cctgtaaata gacctgctgt ttgccgcctt cttcaatcac cgcgccgggt ctggcaccaa
    10321 aggcgatccc gcgcagcacc acattaagac gcgtttctgc cacaggcgca ggttcgggtt
    10381 gttttaccgg tgcggcgacg ggttgatatt tgccaaacca gttttgttgg ctgactaatt
    10441 gcacgtcatt tttatcgaac ggttttgcat ccgcacggag cggtttattc gccgatgagg
    10501 aaaccgccgt atattccgca gaaaaggaga aatagcgcca gagtgaatac gccatttttg
    10561 cagaaataat aagcagcatg agccagaaca tcccgcgcac catttttcgc agatgctctt
    10621 tgtctgtatt aagagattgt ctctggctaa gagtgtgacg aatttttgtc agcaactgaa
    10681 tgagataaat tcgtgcgtca cgaaaaacaa cccgcgccaa tttatatcta cccgacgtta
    10741 tgctttgact attccacagg tggtacgatc cagtttccgc tgatactggt ttcctgtacg
    10801 tgtatcatta atcgttatgc tgtaattaat tccctcggta atcattaatt tcaccgtcgg
    10861 gtcagcgcac atttgtcgtt gatagctggt taaaaaggca tcaggtgtct gcgtggtctg
    10921 agcacccgct tcgctgataa tggtcatttt taccgtcgtg ccgctacttt gcgccagcac
    10981 taaggtatat cccgcagatt taatcggcag gttttggctg atattttgcg cctgtttttt
    11041 cgccagtaaa ctggcatttt cattatggcc ggcacagcca cttaataatc ctgtaacgct
    11101 caacaatagc gatattaata ccctccctgg catttgtttt atcgacatgg taaataatct
    11161 ctaaggttat taataagagt taaaatgtca ctttgataat gacgtggtta tcattaaaac
    11221 aatgcctgta gataaagtgt tgctataccg cccagactta aacacggtcc aaaaggcagt
    11281 gtggttgatc ctctttttgt aataacggca tatatcaggc cgcagcatga tgcgattaaa
    11341 gcaacattgg gtagcgacaa cggccccacc cagccaccta acgcagcgaa aagtaataca
    11401 tcgcccatgc ctaatgcttc tttacgcaga actattccgg ctatccagcg cagggagtaa
    11461 aaagtgataa accccaccag gacgccggta actgcgtctt gtagcgttaa cggactctgt
    11521 tgcgcccatg ccgcaatcag cccggtccac aatacgccct gagtaaaaac atcgggcagc
    11581 cattggttat cgaggtcaat gacgctcgcg gcaatcagcc aggcggataa tatcatcacc
    11641 gccagccccc atccactttc tggccacacc agactcgcca gcaaaaaagc gagtgccgtc
    11701 aataactcca ccagcggata acgcttgctg attttcgcct gacagtcgcg gcagcgccct
    11761 ttgagcatca accatgagag cagcggaata ttgtcacgaa cgcggatggt ctgctggcaa
    11821 tgcggacagt gcgagcgtgg cagcgccagg tttatttttg actgcgtact cggcatttca
    11881 ccgtgaaact ccgccatttg ttggcgcagc atgatggggt aacgccaaat caccacattc
    11941 aaaaaactgc cgatgatcaa tcctccgacg gttgccagta tgggcatccc cgcggagtat
    12001 tgctgaaaaa catcaaaaag catggttaaa ggttgtttgt tgtaacttgc cggatgcggg
    12061 cctgcgggtg tatgccatat ggctttcctc caggcccgat gcgccttatt tcatgccgga
    12121 tgcggcgcga gcgccttatc cggcatacag gcttactcag ctgacatctt atgctcggta
    12181 acctgattaa tggtttccgg cccttgctgc ggtttcggca gattaagcgc cgccagcgtc
    12241 tcgtaagccg actggctcac accgccctcg aagttcatct cgctcgctcc cggtaactgg
    12301 taagcattcg cgcccggatt ccatttctta aagaactccg aaagatccgt ctgagcgacc
    12361 caggaggcac acagcatcag cttgtcggca gcgttaccgt tggattcggc acagtaattt
    12421 ctttcgccaa acttggtttt gccaacatca tcgccgcgtg ctttacggtg catcaactgg
    12481 aacaggttcc agcctttcat cccttcacga tcgctgtaga actcaggcag gtcaccttct
    12541 ggataccact gtttgatatc aaagtttttc tctgcccact ccttcagctg cgcgtacatc
    12601 agcagacggt cgcccgcacc gccgcgcgcc catgcctgac cgttgctctc ctccagatat
    12661 tccggcgcga cagtaatatc gtcagcgaca cggttcatct tgccgagata gcgatcctgc
    12721 atgtacagcg ccagaacgtt gttcgctact tcagttgcgc caggaacagt cagcggcgtt
    12781 tcggcggcgt tgtgccctac ttcgtgccag atcaaccagt cgttcagcgg cgtcgtcggc
    12841 agcgtggtgc tgttcgtcga gaagctgctg ttcattaccg gataacccga gtgcgcatca
    12901 ccgatggaga tctgcacatc gttggtgaaa cgatgcttgt gccccgtcaa gtttttatag
    12961 gtaaacatcc ggtgcttacc gtcttcatca ttacgaccgt agaagtcatt catcgagctg
    13021 gcaaaggtat ccagatcttt agcgaattct gctacaccac cagtgaaatt gctggcctca
    13081 aggttcttct tcggcgtggt ataaacgaaa gcgtctgatt ccagctcgcc cagcggcgca
    13141 ggggagttca gagcgttttt ccattcgccg tctttataga acggcgcttt taccacacca
    13201 gtaaaggtga attcggctga ctcattcttt gggctgttgc ccttgatata aatcagacca
    13261 ccgtaaggca ccttgaaggt caccttatca ttggctttca gatcataggt tttcgtcact
    13321 tttggcggac ggttcagcgc gacttcatgc ttctcgcgtc cggtcagatc gtcggccagc
    13381 gccacggtga cagtcacagg aactgatgca gaagactcaa tggtgacctc tttctgagcc
    13441 ggagcccaca ggccagtaga ctgcatgtta cccgcaaacc atttggtcgg attggagtac
    13501 aggctgatgg tttcagtaac cttctcacct tctgccgata ccgctcccgg atacttctcg
    13561 acatcaactt tgatgttcag atcccaccag gaacgaccca gcatcaggcg cgtcagcggt
    13621 ttttccatat agttgagcgg atagctcggg ttcatcatgc ccgctttatt aacgctcttc
    13681 tcgccgtaga tcatgttgtt atcgaccagc gattttttca gctcatcaga acactgcgtg
    13741 ccgccagtat aggcatcgtt ggcgtagcag ttcaggaact cggtgaacgt tttaaagccc
    13801 agctcgtcat ccttgtcgtt ttcatagcga tattcaattt tattccacag ccagaccgac
    13861 acgttctggt acagacgttc cagatcgacg ctgctcagac gctcaccttt gcgaccattg
    13921 gtccggaagt agagctcatg ctgatacaga cgctgaatgt tggtgcctaa atccgcagcc
    13981 tgcaccatcg cttttgccgt gtcggcgtta aggcttagtt gcgtatactg tggaacatac
    14041 atgccaccag taaccggaac ccccgtgcca ggacgatatt ccagacagtt gacctcgtag
    14101 tggtaagttg ggtccttaca ctcctttaat ccaggaaact tctcaaagat ttttgccttc
    14161 gcagccttca gagaatcctc tgttttatga tcggcctcat caataaaggc ataacgcgtt
    14221 tcctgtttgc catctacatc ttccagccag ctggcaactt ccagcttcgg tttgtcatca
    14281 ggtttgtttt ctacctgata tttccactta acttcccctg tcttactatc gatggtgtac
    14341 ggcagcgcac catctacggc aggataacgt tcatagaccc aaatgcccgt tgcgcgctgc
    14401 tgacgaacgc ggttcggata cccttgcgga tcgttattta ctaccgactt gttcagtgcc
    14461 atcgacagac ctgcggcatc aagcagacgt acaaagccag acgcgctctc ttccttaaga
    14521 ttgctcatca cgttttccat gatcagcacc gatccacctt tgttcagata ggcgatcaga
    14581 tcggtcacat cctgctgagt cagcttcggt ttgctggtat ctgctcgcag cgggattgca
    14641 taaggatcgt taccaacctg agtcacatac tcaaagccgt tgaggatcag cagcggcatt
    14701 tcctgcgggt cgagatcgcc atagctactt aaatgctcaa cagagatacc cgcaaaatcc
    14761 ggatgaaagc cgaactcagc gctgtttcct gtaacctgac catgacgttt gaaatagaca
    14821 gtatccaggt tggtgcctac ggtcatgctg gctttcgcgt ccggcgtcca tttatcgtcg
    14881 gacagatagc gcaacacgtt ctgcataaag tgcttcatat cattagaatc actgttacgc
    14941 gtacactgac cgtttttatc aacaccgcct tcccagctgt aaccgttcgg acaacgcaaa
    15001 atgctgttgt agtgcgggtt accgataacc atcagtttgc cctcaccgac ttgccccagc
    15061 gaaataaacg gcaggttaaa ggtggcggta tcgcgcgtaa cgttttccgg ctcaacgaga
    15121 gaaggcgctt ccgtaatgta cgccagctcg tttttatccc aggcgcgttt ttcgccaaac
    15181 gccagccagt agtttttatc attacgcgcc atcagaatcg ggaaggccgc gttggagata
    15241 ttcaccactg cctgaccgcg cgcattaccg gtactgccat agaagttggt agagtcatgg
    15301 aagacgtgga atttcgtcac tgatttgtaa tctttatcca caccccacag cttgttgata
    15361 acgccctgaa tctgaccttc gttaacattg cgtgctgtca acgagaacca acgctgagag
    15421 ttgcagcccc caagggagtc acaaatcgcc gtatcaatct ctttagcctg cccggattca
    15481 aactgctcaa gaaattcgtt tgtccgctca aaggtttgat caccttcgct caacgcctcg
    15541 ccattggata acgagagatt gataatctcg ttgattacgt tgggatactc ggcaaagacc
    15601 ttgcgcacta cgtccggcac ttcacgctca tcattttttc cggcctggga gtaacgatga
    15661 ataagctgat caatattcgc gccgcgaact tcgtcaccca gttcagtcaa cgcaatggtc
    15721 gacttattgc cgcgcactga gcccagttca aaggtgtcga taccaaagga gatggtttcg
    15781 ccccagctga agttgaattt gccgttttcg ccagttacgc cacggcctga gctggtgtag
    15841 taatttacgc caaccacacc attgcccatg ctatctacca gtcggccttc ggagagaatg
    15901 atttcagtgg gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggaagcgttc
    15961 agatccggtt ttgttcccgg cgtggtgacg ggcacaaccg gtgaagtatg agtggatggc
    16021 gctttatcgg tcgcggcatt gttttccacc tcttcattga ccagcttttt gaactcttcc
    16081 ggtgccagat cgatttgctt atacagcgag tcgaaacgtt tactctcgat cactgaggag
    16141 aacgtcaggc aaacctgttc tgtattcgcc ggacagctgt tactggacgt taccagcgaa
    16201 accgcattgc ttttcttgtc atcggaagct gccagttctt gcgcgtcctc aagactaaac
    16261 gacacttttt caaccgcacg caggctacgc gcagcttctg actgggtgtc gaaggtagca
    16321 attgtcgtgt tacccgccac gcaagtaacg tcctcaccag gtgtaaatgt aaagccatcg
    16381 ctggattcac cattacaagt agcaccagtt acccgctggc ttccgcccag ggtcagataa
    16441 cccgttttcg taggaacagg ttctggctcc ggttctggct caggatcagg tgtcggttcc
    16501 ggcgtaggtt ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg tgttggatca
    16561 ggtttcactt ccggcaaaga ccctgttcca gaatctacag gcggcgtatc ggaggaagat
    16621 ccggagccac cgccatcaca accggctaac agggttgcac tcaaaatagc cgctaaaagc
    16681 gatttcttat atttaaattt cttattcatt aataacgcaa gtgacaaaac aaagttaaaa
    16741 gattcgtaac cggggaaata gttttatgct ttatcttctt taata
//
LOCUS       DAPQZD010000103        16599 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPQZD010000103 DAPQZD010000000
VERSION     DAPQZD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.103"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            88..348
                     /locus_tag="RO454_003224"
     CDS             88..348
                     /locus_tag="RO454_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0935018.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(486..1571)
                     /gene="hcxB"
                     /locus_tag="RO454_003225"
     CDS             complement(486..1571)
                     /gene="hcxB"
                     /locus_tag="RO454_003225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDY0935019.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(1711..2673)
                     /gene="ybiB"
                     /locus_tag="RO454_003226"
     CDS             complement(1711..2673)
                     /gene="ybiB"
                     /locus_tag="RO454_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDY0935020.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(2701..4851)
                     /gene="dinG"
                     /locus_tag="RO454_003227"
     CDS             complement(2701..4851)
                     /gene="dinG"
                     /locus_tag="RO454_003227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDY0935021.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            4955..5155
                     /locus_tag="RO454_003228"
                     /pseudo
     CDS             4955..5155
                     /locus_tag="RO454_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(5388..6749)
                     /gene="rhlE"
                     /locus_tag="RO454_003229"
     CDS             complement(5388..6749)
                     /gene="rhlE"
                     /locus_tag="RO454_003229"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDY0935022.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            6978..7649
                     /gene="cecR"
                     /locus_tag="RO454_003230"
     CDS             6978..7649
                     /gene="cecR"
                     /locus_tag="RO454_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDY0935023.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7652..8647
                     /gene="hlyD"
                     /locus_tag="RO454_003231"
     CDS             7652..8647
                     /gene="hlyD"
                     /locus_tag="RO454_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDY0935024.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            8640..10376
                     /locus_tag="RO454_003232"
     CDS             8640..10376
                     /locus_tag="RO454_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0935025.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10369..11502
                     /locus_tag="RO454_003233"
     CDS             10369..11502
                     /locus_tag="RO454_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0935026.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11513..12619
                     /locus_tag="RO454_003234"
     CDS             11513..12619
                     /locus_tag="RO454_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0935027.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12581..12991)
                     /locus_tag="RO454_003235"
     CDS             complement(12581..12991)
                     /locus_tag="RO454_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDY0935028.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13124..13885
                     /locus_tag="RO454_003236"
     CDS             13124..13885
                     /locus_tag="RO454_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDY0935029.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTCAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13882..15123
                     /gene="clsB"
                     /locus_tag="RO454_003237"
     CDS             13882..15123
                     /gene="clsB"
                     /locus_tag="RO454_003237"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDY0935030.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15123..16079
                     /locus_tag="RO454_003238"
     CDS             15123..16079
                     /locus_tag="RO454_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDY0935031.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16115..>16599)
                     /locus_tag="RO454_003239"
     CDS             complement(16115..>16599)
                     /locus_tag="RO454_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HDY0935032.1"
                     /translation="NTAIAGVIYLFLISLVAIVVASLAPVSAIIVVFSTAGSMFLISM
                     LAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERPIWVISCLMIVLWSGIISHG
                     RNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH
                     "
BASE COUNT         3817 a         4490 c         4430 g         3862 t
ORIGIN      
        1 gattttatct ctcgtaaaaa aacgtgatac tcatcacatc gacgaaacaa cgtcacttat
       61 acaaaaatca cctgcgagag attaattatg aaaactatca atactgttgt tgctgctatg
      121 gctctttcaa ctctgtcatt tggcgtgttc gcggcggaac cggtaacggc atcccaggca
      181 cagaacatga ataaaatcgg cgtggtttct gccgatggcg catccaccct cgatgccctg
      241 gaagcgaaac tggctgagaa agccgcggcg gccggtgcca gtggatacag catcacttct
      301 gccaccaaca acaataaatt aagcggtact gcggtaattt ataagtaatt cgtaagctgg
      361 atcaggcgtt tacgctacgt tcagcacaag tatttccctg tctgtttgcc gacagacgca
      421 tatgctctaa ccctcatgat cctatgttac ccttgtttgc ccgtccgcca ctggacgggc
      481 tttttttagc tggctagctg ctgacagaaa gcctgcaacg tctcttccgg cataccaatc
      541 tgccgcgccg catcacaaat ggcctgccag cttcccgcat ccagtggaat cccctgctcc
      601 tgccgttcgc gacgcgtgtt cacttcccac tcgcccggta gcaaaatcgg cttatcgtca
      661 tcatgcggcg aggctttcac ccactcggca aaggcttcgg tctgcgcact acaatccggc
      721 gcgccgaaca gttccgggtt gataatgata gtggtcatgc agttaagaat ggcatcggga
      781 ctggtttgta acgtttcctg atgcgtcgtt ttaccgccag aaagcgcccc gccaagaatt
      841 tcacacatcg ctgcaagggc gtagccttta tgttcggcaa aggtcaacag cgaacccaac
      901 ggcgactcct gcattaccgc cggatttgtc gtcggcacgc cgttaacgtc aatcaggcaa
      961 cctggcggca cggggacgcc tttatgccag gcgacgcggg ttttgccaaa tgcaatggcg
     1021 ctggtggcgt aatcgagcaa cagcgggaaa ttatctttac gagggaaaac cacacagaac
     1081 ggattggtgc caaagcggct gtcgcgaccg tggaacggcg cgaccattgg aataccgacc
     1141 acgctaacaa agtggataga gacaaacccc gccgctgcac actgctccgc ccagtaaccg
     1201 atacggccga tatgatgcga gttatgtagc gccacggcgg caatgccgtg ctgatgcgct
     1261 ttctcaatcc ccagcgccat cgcttcgtgt gccgcgacct gaccaaatgc gcgatcgcca
     1321 tcgagcgtga ccgccgcccc cgcttcttta acgactttgg catgatggtt aatttgcaga
     1381 tgcccctgac tccaggagcg cacatagctt gggatcatgc caataccatg tgaatcatgc
     1441 cctgccaggt ttgccgcgat caaatgatcg gcgactaatt tcgcttcctg ttcctcacta
     1501 cccatctgac gaaatacagc ctgaataaaa ctgtgcagcg tctgagcatc aaagcgatgg
     1561 ccactttcca tggttaactc ctgtttatgt tatgtgtttg ttgtttttta tgctgcacgc
     1621 cgggcgcggg aaggtcaata aagatgcagg aatttcatat tgcggtgggg taaaagccgg
     1681 atgacatggc tcatccggct gagataaaaa ttaaaatgcc tgattaacgc gcgccaggcc
     1741 gtcgctgata gttgccgctt cacccgtagc catcaggcag caagccatct ggattttcag
     1801 cgattcggga atcggttcgc tgccagccag gcaacgctca atccactgcg ccgtggtttc
     1861 cggatctttt gcttgtggca gtaactcgct accagcagtg tcctgttttt catacagtac
     1921 ccgcatccct tcacggtcaa tgagattgat ctgcgggcag cgctgcggat tagcatacac
     1981 ttcaccttca gtaccatgca taagtagcgc ccgcccgccg atatcgctaa agaacttcgc
     2041 gacgcgtcca atgtattccg gatgcgaaac gctggaaaga cgcagtgcct caccttcggc
     2101 aaatggcgtc gccagtttcg ccagggtatg tgcactgtta cgcacgccca tccgccagcg
     2161 catcgccagt tgtttttcca gcggcgggca aaacgcgccg acaggcataa agacaggttg
     2221 atgttcgtcg agcttcgcct gcgcctgccc gccgtgcaac gttggcgtaa tacccatcag
     2281 ttcgaaaatg gtttcggtca gcacgcgggt tggatcttcg ctaaccccgt gaaccaccac
     2341 aggaaaaccg agtttgtgca gcagaatcgc cagcaacggc gtcaggttgg cctgtttacg
     2401 cgcgccgttg taactgggga tgacaatcgg catcggcttg cctgcaggcg gcgtcagctt
     2461 gatggtgtga ttttgcatgg cttcgtaaaa gccgagcatc tctgcttccc cttccccttt
     2521 gatacgtagc gcaatcaata cgccgcccaa ctcaaggtca gggacttcac cattgagcat
     2581 atgggcatac agaccgcgcg cagtatcccg gtctaaatcg cgcgcgtggt ttttcccgcg
     2641 cccgatctct ttaatgattt tgcgatagtc catttacgac tccttacctg actcacatca
     2701 ttaacgccgc cgacggcgtg gggatttcgt tttttctttc tttttaacta taccttcagg
     2761 gacttccggt tgctctatcg gaaataccgg taatgcatcc agtagtcgct tgccataatt
     2821 ttttgtcagc aagcgtttgt cgtagataac cacttccccc cagcaaccgt ggcttcgaat
     2881 cagtcgccca acctgctgaa tcaggttaaa cgaagcgctc ggcaggcttt gcacctcaaa
     2941 cggatagcgg ttaaggcttt tcagccattc accttcggtg atcaccaccg gactgtcgat
     3001 gggcggaaaa gcgattttgt ggatatgcac ctggctgagc agagcgcctt tcagatcaag
     3061 accttcggca aaggactgta agcccaccag cacgctacgc tcaccgttgg caacgcgttt
     3121 gcggtgcagt tcaactaagc gataacgtgg ctgatcgccc tgcaccagca acatcaggcg
     3181 taaatccgtc acatagtcga gaaagcgctg catcgcacgc ccgctggcaa acagcaccaa
     3241 cataccgaga tgttttttgc tctccacctg ctcacggaaa aaggccgcca tttcagcaat
     3301 gtgctgttct tcgttgtcga tggaaggctc aacgcgcatc cggggaataa caattttgcc
     3361 ctgttcgcag tggttaaagg gcgaatccag cgccacaaag cggtcacccg ctttctcttt
     3421 cagcccactc atctcctgca aacgcgaaaa actgttcagc gaacgcaagg ttgcggaggt
     3481 aacaataatg tgcggaatac tgcgccacag cagcctttcc agttggtcgc taacacgaat
     3541 acccacacag tgaaaccaca gatgtagctg cccttcgcgc tcttcccgcg tcgcccattt
     3601 ggtcaccggc gcaccggaag attgcgccag agacgccagc cgccagagtt tgctttgcgc
     3661 ctcgaacatc cccaacgcgc ggttcatctg caaaatcaac cgatgcaggc gtacaatgtc
     3721 atggctgccg gttttctcac ttaaatcgtt aagaaataac tccgccagac cacgcagcat
     3781 ctcggtgagt tttgccagcc gctggcagat ctccagcact tcatcaggca gttcgcccat
     3841 cgcaaaacgg tgctctgcct cctgcccggc aggcatgtag agattgagaa tgttgtttaa
     3901 cgaggcgata agctcataca gctcttcaca atgcgcattc aaacgttcag ggatcgccag
     3961 cggtgggatg gtcttcgggc gaaactgctc catgcaggtg gcgaccagtt tggtgaacag
     4021 gtccagttgg agccggtacc agggcgcagt gatttcggcg ctcatctcca gcgcatcccg
     4081 cgccacatcc ggcaggtgat gaccttcgtc cagcaccagc agtaaatttt tcgggtcagg
     4141 caataccgct tcgctttcca ttgccgccat caccagcgca tggtttgcca ccaccacttc
     4201 cgcttcctga atttcccgac gagcgacaaa aaacgggcat tcgcgatagt agtaacagtt
     4261 gcggttgagg cagctggctt tgtcggtact caaacgacgc cagagatcat catctatagc
     4321 gatatcagta tgatcgcgca ggccatccca tttataagtg tcgagatcgc ccttcagctt
     4381 cgcacaacgt ttttgctctt cctgattgtt cggcgtcagt tcgtcgtcaa gaaacgccag
     4441 cagatcctgt tgcgtgggct cagtgctggc gagcgctgtc agattacgcg ggcaaacgta
     4501 gcgcccacgc ccaaaagcgg cagtgaattt aagatcggga atgatctttt tcagcagcgg
     4561 taaatctttg ctgtagatct gatcctgcaa tgccacgttg gcggtactta ccaccagcgt
     4621 tttttgctct tcgcgggcaa tggcgatgcc ggggatcaaa taggagagcg ttttcccaac
     4681 gccggtagga gcttcaatcg ccagatgccg cccttcttct ccggccagcg ttttggcgac
     4741 gtccgcaatc atctgccgct gcggcgcacg gggaataaag tcggggatct gttcctgaag
     4801 cgccttatac caggcggcaa tttgcgcttt aagcgcggcg gttaatgcca tgagaaaacc
     4861 tgaaatactg tataaacagc caatattgtg gcatttttcg gttttcaggg gtaatggttt
     4921 ttctgtttct ggaaagcggc tcgcaataac cagcaggatg atgcgtaaag cgcttcgcgc
     4981 taaattcgaa caacatgcag aactgcgtac actcttgctg gcaaccgcgt ccgcaaaact
     5041 ggttgagcat acgcaaaatg atgcttactg gggagatggc ggtaatggtc agggcaagaa
     5101 tcgcctgggg taccttttaa tggcgttgcg cggacaattg gctgcggaga agtaacgcat
     5161 cgggtggctt gtgggcgtta aaacagagaa atctttttgc cgcgctcgca aatggctgac
     5221 aagtgcttgt tgtgaaatgc cggatgcggc gcgagcgcct tatccggcct acaaaaacat
     5281 gcacattcaa tatattgcag agttcacgta ggcctgataa gcgtagcgta tcaggcagtt
     5341 ttgtgtttgt catcagtctc atgccgggca tagcccggca taaaagatta ctgcgcagcg
     5401 gcaggtttac gcggacggcg gcgacgttgt tgttcgcctg ccggtttggc atcgccgaga
     5461 cgacgagacg gtttttctgc gggcttcgcg ttaccagact tcgcaccact ttccgcacgg
     5521 cgtggttgct gttgaccgcg accaccgccc tgtccacgac cgccgccacg ttgctggcga
     5581 ccgttttgga tcggttcggc tttgattgac gggtccggct catagcccag aatcgcaatg
     5641 cgcgggatct cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca
     5701 cacaccagcg acaatgcttc gccagttgcc gccgcacggc cagtacgccc gatacggtga
     5761 acataatctt ccggtacgtt tggtaactca tagttgacga cgtgcggcaa ctcttcgata
     5821 tccagaccac gcgcagcgat gtcagttgcc accagtacac gaatatcgcc cgatttaaaa
     5881 tcggccagcg cacgagtacg cgcaccttgc gatttattac cgtggatcgc cgcactacgg
     5941 atgccatctt tattaagctg ttcagccaga tggttagcgc catgtttggt acgggtaaac
     6001 accagcacct gctgccagtt ccctttccca atcatatgcg acagcaattc gcgtttgcgt
     6061 ttcttatcga caaagtgaac gtgctgggtc acctgatcag acgcagtatt gcggcgtgcc
     6121 acttcgattt ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag
     6181 aaggtcgcgg agaacaacag gttctggcgc ttcgctggca gttttgttaa cacgcgacgg
     6241 atatcgtgga taaagcccat gtcgagcatg cggtcagctt catcaaggac gaggatttca
     6301 acctgatcca gcttcactgc attttgatgt tccaggtcca gcagacgtcc cggcgttgcc
     6361 accagcacat caacgccgcc gcgcagcttc atcatctgcg ggttaatgct gacaccgcca
     6421 aacaccacca gcgaacgaat gttcaggtat ttgctgtaat cacggacgtt ttcgccaatc
     6481 tgcgccgcca gttcacgggt cggggtaaga atgagcgcac gcaccggacg acgccctttg
     6541 gcgtgcggct ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta
     6601 cctgtaccgg tctgggcgct ggccatcagg tcgcggcctt ccagcaccgc agggatcgcc
     6661 tgctgctgaa taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca
     6721 gggcttaaac ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg
     6781 tgtagttttc agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag
     6841 cggagtgtag cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa
     6901 aattcttagc catactggac aactccccta tggagtcata atcttaatca atcatttgat
     6961 taagaatgga ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa
     7021 aacagctgat tgctgcggca ctggcacagt ttggtgaata tggaatgaac gccaccaccc
     7081 gcgagatagc cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga
     7141 aagaagattt gtacctcgcc tgcgcccagt ggattgctga ttttattggc gagcagttcc
     7201 gtccgcacgc cgaagaagcc gaacgtttgt tcgcgcaacc acagccggat cgggctgcca
     7261 tccgtgaact gatccttcgc gcctgcagaa acatgattaa gctgctcacc caggatgaca
     7321 ccgtcaacct cagcaaattt atctcccgtg agcagctctc tcccacggca gcctaccacc
     7381 tggtgcacga acaggtgatt agtccgctac acagtcacct tacacgcctg attgccgcct
     7441 ggaccggctg cgacgctagc gatacccgca tgatccttca tacccatgcg ttgattggtg
     7501 agattctggc gtttcgtctt ggcaaagaaa ctattctgtt acgtaccggc tggaccgcgt
     7561 tcgatgaaga aaagaccgaa ctgatcaacc agacggtgac ctgtcatatt gacctgattt
     7621 tgcaaggatt atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc
     7681 ggtagtagta cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga
     7741 taacggcctg acgctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt
     7801 tggggggcgc gttgagtcgc tggcggtgga cgaaggtgat gcgatcaaag cgggccaggt
     7861 gctgggcgaa ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggtgt
     7921 ttcggtggct caggcgcagt atgacctgat gcttgccggg tatcgagatg aagaaatcgc
     7981 tcaggccgcc gcagcggtga aacaggcgca agctgcctat gactatgcgc agaacttcta
     8041 taaccgccag caaggactgt ggaaaagccg caccatttcg gcaaatgacc tagaaaatgc
     8101 ccgctcctcg cgcgaccagg cgcaggccac gctgaaatca gcgcaggata aattgcgtca
     8161 gtaccgttcc ggtaaccgcg aacaggacat cgcccaggcg aaagccagcc tcgaacaagc
     8221 acaggcgcaa ctggcgcagg cggagctgaa tttgcaggac tcaacgttga tagcaccgtc
     8281 tgatggcacg ctgttaacgc gcgctgtaga gccaggcacg gtgcttaatg aaggtggcac
     8341 ggtatttacc gtctcgctga cgcgtccggt gtgggtacgc gcctatgttg atgaacgtaa
     8401 tcttgaccag gcccagccgg ggcgcaaagt gttgctttat accgatggtc gcccggacaa
     8461 gccgtatcac gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt
     8521 cgaaacaccg gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc
     8581 cgatgatgcg ttacgccagg gaatgccggt aacggtgcaa ttcggtgacg aggcaggaca
     8641 tgaatgatgc cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc
     8701 ccgccgtcgc accgctcgac tgcaccattc acgccggtta tgtgacgggg ctggttgggc
     8761 cggacggtgc gggcaaaacc acgctgatgc ggatgctggc gggattactg aaacccgaca
     8821 gcggcagtgc cacggtgatt ggctttgatc cgatcaaaaa cgacggcgcg ctgcacgccg
     8881 tgctcggcta tatgccgcag aaattcggcc tgtatgaaga tctgacggtg atggagaacc
     8941 tcaatctgta cgcggatttg cgcagcgtca ccggcgaggc acggaagcaa acttttgctc
     9001 gcctgctgga gtttacgtct cttggcccgt ttaccggacg cctggcgggc aagctctccg
     9061 gtgggatgaa acaaaaactt ggcctggcct gtaccctggt gggcgaaccg aaagtgttgc
     9121 tgctcgatga acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg
     9181 tgcatgagct ggcgggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag
     9241 ccgagcagtg ccgtgacgtg ttactgatga acgaaggcga gctgctgtat cagggagaac
     9301 cgacggccct gactcaaacc atggccggac gcagctttct gatgaccagc ccgcacgagg
     9361 gcaaccgcaa actgttgcaa cgggcattga aactgccgca ggtcagcgac ggcatgattc
     9421 aggggaaatc ggtacgtctg atcctcaaaa aagagaccac accagacgat attcgccatg
     9481 ccgacgggat gccggaaatc aacattaacg aaactacgcc acgttttgaa gatgcgttta
     9541 ttgatttgct gggcggtgcc ggaacctcgg aatcgccgct gggcgcaata ttgcatacgg
     9601 tggaaggtac acctggcgag acggtgatcg aagcgaaaga gctgaccaaa aagtttggcg
     9661 attttgccgc caccgatcac gtcaactttg ccgtcaaacg cggtgaaatc tttggtttgc
     9721 tcgggccaaa cggcgcaggg aaatcgacca cctttaagat gatgtgcggt ttgctggtac
     9781 cgacctctgg tcaggcgctg gtgctgggca tggatctgaa agagagttcc ggtaaagcgc
     9841 gccagcatct cggctatatg gcgcaaaaat tttcgctcta cggcaacctg acggtcgaac
     9901 agaatttacg ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa
     9961 tctcccgtat gagcgaggcg ttcggcctga aaagtatcgc ctcccacgcg accgatgaac
    10021 tgccattagg ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggaca
    10081 ttctgtttct cgacgaaccg acgtccggcg ttgatcccct cacccgccgt gaattttggc
    10141 tacatatcaa cagtatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg
    10201 atgaagcgga atattgcgac cgcatcggcc tggtgtaccg cgggaaatta atcgccagcg
    10261 gcacaccgga cgatttgaaa gcgcagtcgg ccaacgatga gcaacccgat cccacgatgg
    10321 agcaagcctt tattcagttg atccacgact gggataagga gcatagcaat gagtaatccg
    10381 atcctgtcct ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca gatcgttcgc
    10441 gatccgagta gctggctgat tgcagtagtg atcccactgc tactgctgtt tatttttgga
    10501 tacggcatta acctcgactc cagcaagctg cgcgtcggga ttttactgga acaacgtagc
    10561 gaagcggcgc tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc
    10621 agcgataacc gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg
    10681 gttattccgg tggatttcgc cgaacagatg gagcgcgcca acgccatcgc accgattcag
    10741 gtgattaccg acggcagtga gccgaatacc gctaactttg tgcaggggta tgtcgaaggg
    10801 atctggcaga tctggcaaat gcagcgagcg gaggataacg ggcagacttt tgaaccgctt
    10861 attgatgtac aaacccgcta ctggtttaac ccggcggcga taagccagca ctttattatc
    10921 cccggtgcgg tgactattat catgacggtc atcggtgcga ttctcacctc gctggtggtg
    10981 gcgcgagaat gggaacgcgg caccatggag gctttgctct ctacggagat tacccgcacg
    11041 gagctgctgc tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg
    11101 tgtatgctgg tgtcggtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg
    11161 ttttttatct ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg
    11221 attacccgca accagttcaa cgccgctcag gtggccctga acgcggcttt tctgccgtcg
    11281 attatgcttt ccggctttat atttcagata gacagtatgc ccgcggtgat ccgcgcggtg
    11341 acgtacatta ttcccgcacg ttatttcgtc agcactctgc aaagcctgtt cctcgccggg
    11401 aatattccag tggtgctgat agtgaacgtg ctgtttttga tcgcttcggc ggtgatgttt
    11461 atcggcctga cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca
    11521 tcgcttatgg acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg
    11581 cgcaattctg attttacccg tgctgattca ggtgatcctg ttcccgttcg ccgccacgct
    11641 ggaagtgacc aacgccacca tcgccatcta cgatgaagat aacggcgagc actcagtaga
    11701 acttactcaa cgttttgccc gcgccagcgc ctttacccat gtgctgctgc tgaaaagccc
    11761 gcaggagatc cgcccgacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc
    11821 tgacttctcg cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg
    11881 gcgtaactcc aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta
    11941 tcagcaggag ctgctggaag gaaaaccgaa acccaacaac agcgagctgg tggtacgcaa
    12001 ctggtataac ccgaatctcg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat
    12061 caccactatc ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg
    12121 tacgctcgat cagttgctgg tttcgcccct caccacctgg cagatattca tcggcaaagc
    12181 cgtaccggca ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg
    12241 ggcgtatcaa atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta
    12301 cggtttatcg ctggtgggat tcggtctgtt gatttcatca ctctgttcaa cacaacagca
    12361 ggcgtttatc ggcgtgtttg tctttatgat gcccgccatt ctcctttccg gttacgtttc
    12421 gccggtggaa aacatgccag tgtggctgca aaatctgacg tggattaacc ctattcgcca
    12481 ctttacggac attaccaagc agatttattt gaaggatgca agtctggata ttgtgtggaa
    12541 tagtttgtgg ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt
    12601 tagacgtaag gtgatgtaac ttcttatctt tcgccagcaa agacaccacc gccgggccgg
    12661 caaggattgc cagccctgca attgccagca aaaagttgtt ttccagtacg cgggacaaca
    12721 gccacagcac aatcagcgct gcaccaaaat accaggtcgt ggtcagctct tccagcacgt
    12781 ctgccacctc acgccagcgt tgctctgggt ggcgctgaaa caccagcatc atcactaccg
    12841 tgacacaata cagtgcgcat aatcctacgc cgacgtgaac cagagtttta ctcatccaac
    12901 ccaccaccag cagcgccact accagtaaat gcaacataat ctgccagcaa cttaggccag
    12961 ttgcgacacg aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac
    13021 ttattcagag taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg
    13081 actacactat ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca
    13141 acaattttcg ttcaaggtgc tcaccatcaa tattcacaaa ggctttaccg cgtttaaccg
    13201 acgcttcatt ttaccggaac ttcgcgacgc cgtgcgtacc gtcagcgccg atattgtttg
    13261 cctgcaggaa gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga
    13321 tacctcgcac tacgagtttc tcgccgacac catgtggagc gatttcgcct acggtcgcaa
    13381 tgccgtatac ccggaaggac atcacggtaa cgccgttctt tcacgttatc ccattgaaca
    13441 ttatgagaat cgtgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg
    13501 cattgtgccg ccgatgaccg ggaaagcgat tcatgtgatg tgcgtacatc tgggcctacg
    13561 tgaggcgcac cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga
    13621 cggcgaatcg gtattggtag cgggtgattt caacgactgg cggcaaaaag ctaatcatcc
    13681 gttaaaagtg ctggccgggc tggatgagat ttttacctgc gcccacggac gtccggcgcg
    13741 cacgtttccg gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag
    13801 cgccagcgcg ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc
    13861 tttaagtgcg gagattcatt tatgaaatgt agctggcgcg aaggcaatat gatccagttg
    13921 ctggaaaacg gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcgcaagaa
    13981 cgcatcattc ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca gctgcatgcg
    14041 gcgctattgg cagcagcgca acgcggggta aaagcggaag tcttgctgga tggctacggt
    14101 tcgccggatc tcagcgatga atttgtcaat gaactgacgg cagctggcgt ggtgttccgc
    14161 tactacgatc cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat
    14221 cgcaaaattg tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc
    14281 gagcatatgt ccagctacgg cccggaggcc aaacaggatt acgcggtacg cctcgaaggg
    14341 ccgattgtcg aagatattct ccagtttgag ctggaaaacc tgccaggaca aagcacggcg
    14401 cgacgctggt ggcgacgcca tcataaagcg gaagagaacc gccagccggg agaagcgcag
    14461 gtattgctgg tctggcgcga taacgaagaa catcgcgatg atatcgaacg tcactatctg
    14521 aaaatgctca ctcaggcgcg gcgggaagtg attatcgcca acgcctactt cttccccggc
    14581 tatcgttttt tacacgcctt gcgtaaagcg gcacgacgcg gggtgcggat caaactgatc
    14641 attcagggcg aaccggatat gccgattgtc agagtcggcg cgcgtttgct gtataactat
    14701 ctggttaaag gtggcgttca ggtgtttgag taccgccgcc gcccgctcca cggcaaagtg
    14761 gcattgatgg acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg
    14821 tcactgaatc tcgaagcaaa tgtcatcatc cacgatcgta attttaacca gacgctgcgc
    14881 gataatctga acggcattat cgccgcagat tgtcagcagg tcgatgagac catgctgccg
    14941 aaacgcacct ggtggaacct gactaaaagc gtgctggcgt tccactttct acgccacttc
    15001 ccggcgctgg tgggctggct tccggcgcac acgccacgtc tggcgcaggt tgatcccccc
    15061 gcacaaccga caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc
    15121 tgatgagtaa atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt
    15181 ttatcgcggt gattgtgttg ttggtggtct acgccaaaaa agtggactgg gaagaggtct
    15241 ggaaggtcat ccgagactac aatcgcgttg cactgctcag tgcggtcggg ctggtggtcg
    15301 tcagctatct gatttacggt tgctacgacc tgctcgcccg tttctactgc ggtcacaaac
    15361 tggcgaagcg ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacactca
    15421 gtacctgggt cggcggcatt ggtatgcgct atcgtttgta ctcccgactg gggttaccgg
    15481 gcagcactat tacgcggatt ttttcgctca gtattaccac caactggcta ggctatattt
    15541 tgctagcggg ggttatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg
    15601 atcaaactac gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt
    15661 ggttttgcgc tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc
    15721 cttcgtggaa attcgccctc gcccagatgc tgatttccag cgttaactgg atggtaatgg
    15781 gagcgattat ctggctgtta cttggtcaaa gcgtaagcta tttctttgta ctgggcgtgt
    15841 tgctggttag tagtattgct ggcgtcatcg tgcatattcc ggcgggaatt ggtgtgctgg
    15901 aagcggtgtt tatcgcgcta ctggctgggg agcatacctc caaaggcaca attatcgccg
    15961 ccctacttgc ttaccgtgtg ctgtattact ttatcccact gctgctggcg ctgatctgtt
    16021 acctgttgct ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa
    16081 gtatagttaa gttctttagt atgtgcattt acggctaatg aaaaaaccgc gtatgccttt
    16141 gccagacaag cgttatagct atagcagcaa gaatcccaaa gaaaccgata aaatagtaat
    16201 agagtgttaa tgcacctata aaagcacaac gaaccggact ccataattct tcactatggc
    16261 tttttcctgt cagttcaagg agcttatttc gtccatgcga gataatgcct gaccataaga
    16321 caatcattaa gcaacttatt acccaaatgg gtcgttcact cattaatgtg gcattcacga
    16381 tgattaccag ggccaaccct gtcaacgtca tcataatgat aaaacgatga gaaccaggat
    16441 caaatttaaa caatagacct gccagcatac tgattaaaaa catcgatccc gcagtgctga
    16501 acacgacgat aatggcagat acaggcgcta aactcgcaac gacaatcgcc accaaagaga
    16561 ttaaaaacaa atatattacg cccgcgatgg cggtattta
//
LOCUS       DAPQZD010000104        16563 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPQZD010000104 DAPQZD010000000
VERSION     DAPQZD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.104"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            230..811
                     /gene="mdaB"
                     /locus_tag="RO454_003240"
     CDS             230..811
                     /gene="mdaB"
                     /locus_tag="RO454_003240"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDY0935033.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            842..1156
                     /locus_tag="RO454_003241"
     CDS             842..1156
                     /locus_tag="RO454_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDY0935034.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1201..2088)
                     /locus_tag="RO454_003242"
     CDS             complement(1201..2088)
                     /locus_tag="RO454_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDY0935035.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2085..3032)
                     /locus_tag="RO454_003243"
     CDS             complement(2085..3032)
                     /locus_tag="RO454_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDY0935036.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3043..4080)
                     /locus_tag="RO454_003244"
     CDS             complement(3043..4080)
                     /locus_tag="RO454_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDY0935037.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4089..5072)
                     /locus_tag="RO454_003245"
     CDS             complement(4089..5072)
                     /locus_tag="RO454_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDY0935038.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5069..5878)
                     /locus_tag="RO454_003246"
     CDS             complement(5069..5878)
                     /locus_tag="RO454_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0935039.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            6252..8393
                     /locus_tag="RO454_003247"
     CDS             6252..8393
                     /locus_tag="RO454_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDY0935040.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(8457..10349)
                     /gene="parE"
                     /locus_tag="RO454_003248"
     CDS             complement(8457..10349)
                     /gene="parE"
                     /locus_tag="RO454_003248"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDY0935041.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRLDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10378..10959)
                     /gene="yqiA"
                     /locus_tag="RO454_003249"
     CDS             complement(10378..10959)
                     /gene="yqiA"
                     /locus_tag="RO454_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDY0935042.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(10959..11786)
                     /gene="cpdA"
                     /locus_tag="RO454_003250"
     CDS             complement(10959..11786)
                     /gene="cpdA"
                     /locus_tag="RO454_003250"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDY0935043.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(11811..12233)
                     /locus_tag="RO454_003251"
     CDS             complement(11811..12233)
                     /locus_tag="RO454_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDY0935044.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(12234..12863)
                     /gene="nudF"
                     /locus_tag="RO454_003252"
     CDS             complement(12234..12863)
                     /gene="nudF"
                     /locus_tag="RO454_003252"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDY0935045.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            13068..14549
                     /gene="tolC"
                     /locus_tag="RO454_003253"
     CDS             13068..14549
                     /gene="tolC"
                     /locus_tag="RO454_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDY0935046.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            14697..15368
                     /locus_tag="RO454_003254"
     CDS             14697..15368
                     /locus_tag="RO454_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDY0935047.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            15374..16534
                     /locus_tag="RO454_003255"
     CDS             15374..16534
                     /locus_tag="RO454_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDY0935048.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         4386 a         4407 c         3982 g         3788 t
ORIGIN      
        1 tttccgaaaa actcctgtat catttgcata atacatctct cctgtgggag cttatgggaa
       61 gttgatttaa gtattgtcat tttcagaaaa tatttatccc gcatcggtga atcagagaaa
      121 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
      181 ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag gttaaaaaaa tgagcaacat
      241 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
      301 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca ttgttcgcgc
      361 cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
      421 ctggcagatg cctggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
      481 tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc
      541 atcaaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctgac
      601 ctggaacgca ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggtgt
      661 tgacggtgtg tacctgccgt tccataaagc aaaccaattc cttggcatgg aaccgctgcc
      721 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
      781 tcgcaagcat cttgtggaaa tttttggtta actagagctc tggctttaga aggagttaac
      841 catgattaca gtaatcgcag aaattcgtac tcgtcctggt caacatcacc gtcaggcagt
      901 gttggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
      961 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
     1021 cgtgatgatt gagcagtggg aaagcatcgc gcatcttgaa gcgcatctgc aaaccccgca
     1081 catgaaagca tatagcgaag ccgtaaaagg tgacgtgctg gagatgaata ttcgtatcct
     1141 gcagccgggg ctttgacatt tctaaaaaga ggccggtgct gctaccggtc tcagcccaga
     1201 ttagccattc tttcttcccg ttttcagtcc tcgatggaaa tcgttaattc gcttaatggt
     1261 tttaaccgca cgctgcggca ctgcggaagc taccgaccag acaatcattt ccagacacaa
     1321 caataccgta ccatgtagcg gcgttttccc tttttcatcc ccgcgcggaa catgaatcac
     1381 gatactggcg tctttgctaa aacgtgaatc cagcgcgttt gtcagcaaaa taacagggat
     1441 ccccagccgc ctcgcctcac gaagcgttgt cagcccttcc cggtgcgcag atttttgcgc
     1501 catcatgatc agaacatcgc cacgttgaag cgcgatcagt tgctcggcaa ggccaatacc
     1561 cgtacggttt agtgccgtgg caggcaaacc tatgcggttg aatagtcgtg cggtatagtc
     1621 cgccagaata cccgaagcgc caatgccaaa aattgcgact tgccgtgcct gcaccaacaa
     1681 cgctacagct tgcgcaagcg cgtggcggtt ttcaggctct gacaggacat tacaggtata
     1741 caaatgccct tcaagaacaa agtcgattgc ggtgttaaca tcgctcgtca agttactcac
     1801 cgtcgtagac atcttttcgc tggaactaag ggctggacca agccactgtt ccagagtgcg
     1861 tttcagatca cgcagcccgg caaaccccag ggcctgtata gccctgataa ctgtggcatc
     1921 ggaagtgttc aacgtggccg caatttccat cgccgtttgc tcgagtacca cttcacgatg
     1981 ctcattaatg tagctggcga caactcttaa cccaggcgtt aactgactcg cgcgagtgcg
     2041 aaaacgctca ccaaaaatat caacccgcac tttatctttc tggatcattt agcctccggt
     2101 agaggtcgcc ctgcaatttg tgattgtatc tgcgcagcca tcagccccag agatgcaaag
     2161 gagttagaaa ttgcctcttc acgagaaatc agcacataac gcccggaacg acaggcagtc
     2221 agaaactgac accatcccgg catgactttt tccattgctg ccagctcatc ctgaggttta
     2281 ccaccggtgt caccgcgcca ggttgcaaaa acaaaatcgg catccagttc aggtaaacgc
     2341 tccgcgctca catccattcg cccaccttca ggaatgctct caatcagcgg cggaaaacga
     2401 aaaccagcat cgcgtaatac ccgtcccagc gaatgatagc tatgcatcac attaattttt
     2461 ccctggtttg cctgaatcac cgataccgtt attttttggc tgtccagcgt cgccttcagc
     2521 gcattgattt gtgcctgata gcggcgttca agtatcgcca gctgcgactg cgttcctgta
     2581 agttctgcca gcttgcgata gatttctggc gcgccccctt tgagatgatc aatgctcacc
     2641 gtgggcgcaa ttttttcaag ttgttcaatg ggcgtattac gggtcggttc tgtgatgatc
     2701 aagtcaggct ttgccgccac aatcgcttca atatcaatat cagcggtacc aataaaggcg
     2761 atcgatgaat tatcaaagtc gacacctgtc aataacgcgc cagatcgaat gaaatggcta
     2821 ccgtcaggcc gagttcgccc gtggctggcg acaggaggta caccgagctc aatcagagga
     2881 atagtgatat caagatcgtg taaagagaca attctctttg gatgagcagg aacagtgact
     2941 ttgcgcccta aatcatcagt aaatacctga acaggttctg ttgcacggga aaaaaatgac
     3001 aacagtatca gcagagaaaa aaataaacgc atataactcc cgttaaaaac tgttcctgcg
     3061 ctgccagagc agtagtaaaa aaaatggtcc accaatcagt gagataatga ttccggcagg
     3121 cagttgcaaa ggcaaaaaag ccaaacgacc aatgctatcc gccagaataa ctaacaacgc
     3181 ccccgatacc gcgctcccca gcagtaatgt cgtctgcccg ccacgtagaa taaaacgtga
     3241 tatatggggt gctatcaatc cgacaaaacc gatgttacca acacacgaaa cgcagacggc
     3301 tgtcaggatt atgggtgcgg cgacacgtaa caacgccaga cgagaactat tcacccctaa
     3361 cccggttgca acctgatgac caagcagagc gatatccgca gtcctggcgg tgaacagcaa
     3421 taaaagagcg gctggcaaca tccaacaggc tgaaataccg actaacatcc agttagctgc
     3481 atgcaggctt cccgataacc acatcaatgc agtttgtaca tctcgaacat ctgcagtggt
     3541 cataaataca ccgataccgg cggcgaaaaa ccaggaaaca ccaataccaa tcagaacaaa
     3601 tcgcgggcgg gaaatatcgc gcgcacaaaa aatcacgatt aaagccacca acaggccacc
     3661 agcaagacca gccactggac gccagaacat tcccagcatt ggaaactgga aaatcagcca
     3721 cagcacagcc acgctacatc cttctttgac gccgattaaa ccaggatcgg caaggccgtt
     3781 acgtgcaatg gattgcattg cggcccctgc catacctaac atggccccgc atagcaatgc
     3841 catgatgact ctcggcaagc gaatatcctg cacaatataa cgtgcgtctg tgcttacgtt
     3901 ttctggagca aacaacgctc gtcctacttc cgatgccgga acagagaaag aaccctgcat
     3961 cagaccgaat atcagtattc caaccgcaag caggaacagc ccaccaagca acagcagatt
     4021 tttagggcgc accagtgtcg aaaacttacc aacacgtagt gggcgtagcc cgacgttcat
     4081 tatccgactc atttgaacat cctcgtcgcc ataatgacaa aaacaggcgc accaactaac
     4141 gccgtcatga caccggtcgc taactcatgg ggcgtgaaaa gcgtccgggc aataatatct
     4201 gccagtaata acaaaagcgc gccgacacaa gctgaaagag ggagcagaac gcgcagatcc
     4261 gctgatacca gacgccggac aatctgcggt actagcaggc cgacaaaacc aatggggcca
     4321 gcaatcgata cggctgcgcc acagagcaac gcgatcgcaa gcagggtgaa tgttcgcgtg
     4381 cggagtacag agacgcccag cccctgcgcc atgcgatcgc ccagtgccaa catgttaagg
     4441 gagggtgcaa ggtaaagggc taacacaaat ccccccagag aaaaccaggc ggacgtaccc
     4501 agcgtcgccc aatcctgccc cgctaaatca cctgctagcc atgtccgcat cgccagcagt
     4561 gtttgttcgt caagaatcag gattgcagcc gttattgagg acacgaacgc cgatagcgcc
     4621 acgccgcata aagtaacttt cattggcgtt aaccctgaac gccctgcgga ggaaagcagc
     4681 aatatcaaca ggaacaataa cgcgcccccc gtagatgcca gcagtgggcg tccaaccgga
     4741 aaggccagtc caagcgcact ggcggcaacg acagcaagag cggctccggc atttaagcct
     4801 aaaatatgtg gttcccccaa tgggttacga atgacagcct gcaacaaagc accagcaaca
     4861 ccaagagaag caccggtaag caatgctgcc gctaaacgcg gcaaacggag tcttacaatt
     4921 acgttgtgat cgaagttttg cggatcaaaa tgaagaaatg cctgcaacac cgtttgtggt
     4981 gcaataaatc gggcaccaat ccctggatat accacgcatc ccaccagcag taaggcgagg
     5041 aaaattacag taaccagccc tgtgcgcatc atactttttc tacaccacga aacggcatga
     5101 agaacggctt tcccgtcaga ggatttattg atgcgtgaac atcaacgtca aagacagctt
     5161 ttaccagttc aggcgtgcaa tgctccccct cattcaacac acgcaccact ttcccctggc
     5221 gtaaaaatat caacgtatcg ccataattga cggcaaaatt cagatcatgc aacaccacga
     5281 cgactgtacg cccgtgatga cgcgttaaat catgtaataa ctcaagaatt tcaacctgat
     5341 aacgtaaatc aagccaggtt gtgggttcat ccagcaggat ataaggcgtc ttttgcgcca
     5401 acaccatcgc aatccagcaa cgctggcgct gcccaccaga gagtttttca acaggtaaat
     5461 gtgcaaactc ctgcgtaccg gttaatttca gggcctcttc caccgcctgc tcatcggcgt
     5521 cgctccattg acgtatgaaa ttttgccagg gaaaacgccc acgcgacacc agttcataaa
     5581 cggttaagcc ctccggcaat aatggcgact gcggcaagat cccaagtcta cgggataacg
     5641 ctttcgttgg ttgctcgtga atcgctttgc catcaagcaa tatcgaaccg cccataggtt
     5701 gcaagatacg tgcaatggtg ctgagcaacg tcgactttcc acagccgttt gccccaacca
     5761 gaacggtcat tttttctgtg ggaacggaga aagaaacacc atcaacaatg attttcttgt
     5821 gatacccggc agaaacattg tcgaggatca gcccctgccc ttttgcgtta tgtgccacgt
     5881 gtacgccaac ttaatcaaag acaaacaaaa ataaaactca ttctcctttg agagtttgac
     5941 gccacaaaat gtagtagtca agcagttaaa agccacacaa aaacaaggta aataacaggt
     6001 aaaatatttt tatacataaa aataaaaaca ttaaatatca ataaattaaa agcaatcaaa
     6061 ttgtataaac ctttcataaa aacacatgta gcaatttttt gttatttctc atttactact
     6121 acattactgc gtgattgaat gattctcaat tacatatccg atggcgaaat aacaaacgga
     6181 tagtgcttag tgagcaataa gaaacaattt tcactcgcga caatccgcga gtttttggga
     6241 taacagacta aatggctatg ttcacacctt cattctcagg actcaaaggt cgggcgctct
     6301 tttcactgct ttttgcggca ccgatgattc atgcaacaga ctctgtaacg accaaagatg
     6361 gcgaaacaat cactgttaca gcagatgcaa ataccgcaac tgaggcaacc gatggttatc
     6421 aacctctgag cacctctacg gcgacattaa ccgatatgcc gatgctggat atcccgcagg
     6481 tggtcaatac ggttagcgat caggttctgg aaaaccagaa tgcgacaacg ctggatgagg
     6541 cgctttataa cgtcagtaac gtggtacaga ccaatacatt aggcggaact caggacgcct
     6601 ttgtacgccg tgggtttggc gcaaaccggg atggctccat catgaccaac ggtctgcgaa
     6661 ccgtacttcc tcgcagtttc aacgccgcca cagaacgtgt ggaagtgcta aaaggcccgg
     6721 cctccacgct gtatggcatt ctcgatcctg gcggactgat taacgtcgtg accaagcgcc
     6781 cggaaaaaac attccatggt tcggtttcag ccacctcctc cagttttggt ggcggcactg
     6841 ggcaacttga tatcacaggt cccattgaag gcactcagct ggcgtatcgc cttaccgggg
     6901 aagtgcagga tgaagattac tggcgaaact tcggtaaaga gcgcagtaca tttattgccc
     6961 cgtcactcac atggtttggt gataatgcaa cagtaaccat gctctattcc catcgggact
     7021 ataaaactcc gttcgatcgt ggaacgattt tcgaccttac gacgaaacag cccgtaaacg
     7081 ttgatcgaaa aatacgtttt gacgaaccgt ttaatattac agatggtcag tccgatctgg
     7141 cgcaactcaa cgcagaatat catctcaata gccagtggac agcacgcttt gattacagct
     7201 acagccagga taaatacagc gataatcagg cgcgtgttac cgcatatgat gcaacgacag
     7261 gaacactgac acggcgtgtt gatgcaactc agggctctac ccagcgtatg catgctactc
     7321 gtgcggatct gcaagggaat gttgatattg ccggattcta taatgagatt ctgggtgggg
     7381 tgtcatatga atattatgat cttctgcgta cagatatgat tcgctgtaaa aaagctaaag
     7441 atttcaatat ttacaaccct gtttatggta ataccagcaa atgtacaacg gtttcggcgt
     7501 cggacagtga tcagacgatc aaacaggaga gctactcagc gtatgcacag gacgcactct
     7561 atctgaccga taactggatt gccgtcgccg ggatccgcta tcagtattac acgcagtatg
     7621 cgggtaaagg ccgtcctttt aatgtcaata ctgacagccg cgatgaacaa tggactccca
     7681 aactggggtt ggtctacaaa ctgacgccat cggtatcctt atttgccaat tattcgcaaa
     7741 catttatgcc gcagtcgtca attgccagct acattggcga tcttccaccg gaatcatcta
     7801 atgcttacga agtcggggca aaattcgagc tattcgatgg tatcaccgca gatattgcgc
     7861 tgtttgatat ccataaacgt aatgtgttgt ataccgaaag tattggtgat gaaaccatcg
     7921 ccaaaacggc aggccgtgtt cgttcaagag gggtagaagt cgaccttgcg ggagcattaa
     7981 ctgaaaacat taatatcatt gccagctacg gctataccga tgctaaggtt ctggaggatc
     8041 ctgattatgc agggaaacca ttgccgaatg ttcctcgtca taccggttcg ctattcctga
     8101 cctatgatat tcataacatg ccaggcaata atacgctgac atttggcggt ggtggacatg
     8161 gtgtaagccg tcgttcggca accaatgggg ctgactatta tctgccaggc tattttgttg
     8221 ccgatgcctt cgccgcatac aaaatgaaat tgcagtatcc ggtcacactg caattaaacg
     8281 tcaaaaacct gtttgataaa acgtattaca cctcttccat cgccacaaat aatctgggga
     8341 atcagattgg cgatccgcgt gaagtgcaat tcacggtgaa aatggaattt tgatcaaaaa
     8401 agcccggtat acacttgtat accgggctat gttttattct tctggcggga ggattatcag
     8461 acctcaatct ctgccatgtc gcctttttct tgcaaccagt tgcggcgatc ttccgagcgt
     8521 ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc atcatcgata
     8581 gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcgcgcaa ttgcatcggg
     8641 ttcatttccc cgagaccttt aaaacgctgg acgtttggct tgcctttctt gcgttttaat
     8701 tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc tttcccgagg
     8761 tcaagacggt agagcggtgg cagtgcaacg taaacgtgac cgtgtttcac caatgcgcgg
     8821 aagtgtttta caaacaaagc gcagagcagc gtggcaatgt gcagaccatc ggagtcagca
     8881 tccgccagga tacagatctt gccgtaacga agctggctca aatcgtcgct gtcaggatcg
     8941 ataccgatag ctaccgaaat atcgtgcact tcctgcgaag ccagcacttc gtcggaagag
     9001 acttcccagg tgttaaggat cttacctttc agtggcatga tcgcctgata ttcgcgatcg
     9061 cgcgcctgct tggcagatcc gcctgcggag tcaccttcca caaggaatag ttcggtacgg
     9121 ttaagatcct gcgcggtaca gtcagccaat ttaccaggca gcgccgggcc gctggttagc
     9181 tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga aatcgccatc
     9241 tctgccaaca gctccgccgc ctgaacgttt tggttcagcc acaggatgaa ggcatctttc
     9301 accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgttcttt ggtctgcccg
     9361 gcaaactgcg gatcctgcat ttttactgac agcacatagg cgcagcgatc ccagatatct
     9421 tccgccgaca gctttacacc acgcggcaga atgttgcggt attcacagaa ctcacgcatc
     9481 gcgtccagca ggccctgacg caggccatta acatgggtac cgccctgcat cgttgggatc
     9541 aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag tagcgcccag
     9601 tccaccgctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg cagcgtcggt
     9661 aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca ccagcgctgt
     9721 tcggtgttgt tgatctcatc tttaaaagtg atctcaacgc ccgggcacag taccgctttg
     9781 gctttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca
     9841 tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt
     9901 aaatcttgca ccttttcgcc attttcaaag gcgatgttat agatctgacc atcgcggcgt
     9961 acgttaactt ctacgcgctt cgacagggcg ttaactaccg aaatccccac gccatgcagg
    10021 ccgccagaga actggtaatt tttgttagag aatttaccgc ccgcgtgcag acggcaaaga
    10081 atcagttcaa ccgccggtac cccctcttcc gggtgaatat ctaccggcat cccgcgccca
    10141 tcgtcaatca cttctaacga ctggtcggca tgtaagatta catccacgcg ttttgcgtgg
    10201 cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg gttagggcga
    10261 gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt gagtacctca
    10321 atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca
    10381 gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga agcccgtgaa
    10441 tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg ccaccgcctg
    10501 gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc
    10561 cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta gcacatattg
    10621 ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcagtt caaacgggcg
    10681 caccgccggg ttgaccacca ctgcgggcag cataaaacat tgcgacaacc aggtggcgta
    10741 atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat
    10801 ggattccagc agttctgccg cgtcggaagg atacggcggc agctgcggaa tgatcatctc
    10861 aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg
    10921 cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc tgaagcggta
    10981 tcaggttgga agcgtgcgtc cgccaggcga tgcacctcgg tggtcagcgt gccatcagca
    11041 tgtaactcaa gagtacgcca gccgggcgcg atggtatcca gcgtaaagtt ggaacagtgc
    11101 ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga
    11161 tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa cttcgccagc
    11221 acggtatcca gttcgcccgc gttacgcagg ctgtgttgat caagccaact acaacccgca
    11281 ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta
    11341 cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg
    11401 ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc cggggagata
    11461 cccgcatcct gtaacgcact gtacatcgcg ggctggaaat cgtggttgcc cggcagccag
    11521 acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc cgcagaggat
    11581 tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg gtgtggccga
    11641 atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag ggcttcgtgc
    11701 ttttgtgcaa acaggtgagt gtcggtaatt tgtaaaatcc tgactctggc ctcaccagcc
    11761 agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg ctaataaacc
    11821 ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa aaactgatta
    11881 atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata ccgcgctttg
    11941 aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg atacagacgc
    12001 accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc tattgtcacc
    12061 agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt tgccacctga
    12121 tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag
    12181 ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt catttatgcc
    12241 cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat gaccgacgct
    12301 gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct taccacatga
    12361 acgcgaatat cttcgttttc atcagccaga ccgtgaatac cgcttgcggt cgtggcgtcc
    12421 acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc caggaaactt
    12481 aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc gcgacgggca
    12541 acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag tagccaaggg
    12601 gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac ttcatcacgc
    12661 actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc
    12721 tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc taatgaaaaa
    12781 aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc aaatgtaacg
    12841 ggcaggttgt ctggcttaag cattgttaat gtcctggcac taatagtgaa ttaaatgtga
    12901 atttcagcga cgtttgactg ccgtttgagc agtcatgtgt taaattgagg cacattaacg
    12961 ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat ccgcaataat
    13021 tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg aagaaattgc
    13081 tccccattct tatcggcctg agcctctctg ggttcagttc gttgagccag gccgagaacc
    13141 tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag tctgccgccg
    13201 atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg ccacagctag
    13261 gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc atcaactcta
    13321 acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg aaatggcgtg
    13381 cgttaacgct gcaggaaaaa gcagctggga ttcaggacgt cacgtatcag accgatcaac
    13441 aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgcg attgacgttc
    13501 tttcctatac acaggcgcaa aaagaagcga tctaccgtca attagatcaa accacccaac
    13561 gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgca cagtacgata
    13621 ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta gagcagctgc
    13681 gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa aactttaaaa
    13741 ccgacaaacc acagccggtt aacacgctgc tgaaagaagc cgaaaaacgc aacctgtccc
    13801 tgttacaggc gcgtttgagc caggacctgg cgcgcgagca aattcgccag gcgcaggatg
    13861 gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc tcttatagcg
    13921 gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg ggccagaaca
    13981 aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac tcgcaggtga
    14041 aacaggccca gtacaacttt gttggtgcca gcgagcaact ggaaagcgcg catcgtagcg
    14101 tcgtacaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt agcattaacg
    14161 cctacaaaca agccgtagtt tccgctcaaa gctcattaga cgcgatggag gcgggttact
    14221 cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcta
    14281 agcaagagct ggcaaatgcg cgttataact acctgattaa ccagttgaat attaagtcag
    14341 ctctgggtac gttgaacgag caggatctgc tggcactgaa caatgcgctg agcaaaccgg
    14401 tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg
    14461 gttatgcgcc tgatagcccg gcaccagtcg ttcagcaaac atccgcacgc actaccacca
    14521 gtaacggtca taaccctttc cgtaactgac gacgacgacg gggcttcggc cccgtctgaa
    14581 cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt
    14641 tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga
    14701 aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga
    14761 caccagtcgc tctcgcggtt gccactgttt ttatgctggc gggctgtgaa aagagtgatg
    14821 aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg
    14881 ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat
    14941 acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgccag caggcaccag
    15001 cccaggctgg catggcacca gaaaaccagg cgcaggccca gcaatccagc gggagtttct
    15061 ggatgccgct gatggccggt tacatgatgg gacgtctgat gggcggcggc gcgggatttg
    15121 cacagcagcc gctgttctcc tcgaaaaacc cggccagccc ggcttacggt aaatataccg
    15181 acgcgaccgg taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga
    15241 cggcaatggc accaaaaccg gcgaccacta ctactgttac ccgtggcggt tttggtgaat
    15301 ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg
    15361 gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc gcgaaaaagc
    15421 ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc
    15481 ttactacaag ttgaccctct ctcaggttga aaagctggaa gaagtcaccg ccgaactgca
    15541 ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt
    15601 ccgcatacca aaacacacct ggagttttgt gcgccagtca tggctgacgc accagccatc
    15661 gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac ttctggaaaa
    15721 taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga
    15781 agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca gtctgcaaga
    15841 aaagctgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct
    15901 cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc
    15961 aacggaagct gaaatcgcca ccgagttcct ctacattgac gatatcgggt taggtgaaaa
    16021 aggtcagttc acggatttac aggatcaggt gatttccaac ctgttcaagc tgtatccgtg
    16081 ggaatttatg ttgcgtgaga tgttctccac caagctggag gatgcaggcg tacgctggct
    16141 ggaaccggcg tggaagagca ttatctccaa caaagcgctt ctaccgctac tgtgggagat
    16201 gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat
    16261 ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga
    16321 gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca
    16381 gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt
    16441 gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcattgatca cccaggatat
    16501 gtctcggttt tatccacata tttttgttga gtaatacagc ttaatattgc cccgataagc
    16561 ggg
//
LOCUS       DAPQZD010000105        16549 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPQZD010000105 DAPQZD010000000
VERSION     DAPQZD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.105"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            149..2146
                     /gene="betT"
                     /locus_tag="RO454_003256"
     CDS             149..2146
                     /gene="betT"
                     /locus_tag="RO454_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDY0935049.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATGLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2209..3486
                     /locus_tag="RO454_003257"
     CDS             2209..3486
                     /locus_tag="RO454_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDY0935050.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATHADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            3734..4390
                     /locus_tag="RO454_003258"
     CDS             3734..4390
                     /locus_tag="RO454_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0935051.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYFKSKEDYAQTVLNAYDSFFEHKLKKHLHESSCTPMVRLENFI
                     NDACEGIKKYNFTRGCLVGNMMQESPGLPQSFIKVLQNILESWQALVAACLSDALSSG
                     EISSNMNNTQLAAIFWSGWEGAVMRSKLYCSTEPVYDFWSYFKTSVRYQSSQEATTGQ
                     "
     gene            complement(4571..4678)
                     /locus_tag="RO454_003259"
     CDS             complement(4571..4678)
                     /locus_tag="RO454_003259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDY0935052.1"
                     /translation="MGNMAPFILRGVTLKGIDSVMFAKEKRAAIWQQAV"
     gene            5676..5902
                     /locus_tag="RO454_003260"
                     /pseudo
     CDS             5676..5902
                     /locus_tag="RO454_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(5904..6686)
                     /locus_tag="RO454_003261"
     CDS             complement(5904..6686)
                     /locus_tag="RO454_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDY0935053.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            6992..7912
                     /gene="rbsK"
                     /locus_tag="RO454_003262"
     CDS             6992..7912
                     /gene="rbsK"
                     /locus_tag="RO454_003262"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDY0935054.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            7940..9256
                     /gene="fucP"
                     /locus_tag="RO454_003263"
     CDS             7940..9256
                     /gene="fucP"
                     /locus_tag="RO454_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDY0935055.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            9268..10281
                     /locus_tag="RO454_003264"
     CDS             9268..10281
                     /locus_tag="RO454_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDY0935056.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVMGFGHHYLAQPTVVLHRSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDLPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(10552..12165)
                     /locus_tag="RO454_003265"
     CDS             complement(10552..12165)
                     /locus_tag="RO454_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDY0935057.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(12196..12546)
                     /gene="tnpB"
                     /locus_tag="RO454_003266"
     CDS             complement(12196..12546)
                     /gene="tnpB"
                     /locus_tag="RO454_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDY0935058.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(12543..12968)
                     /locus_tag="RO454_003267"
     CDS             complement(12543..12968)
                     /locus_tag="RO454_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDY0935059.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            13306..13542
                     /locus_tag="RO454_003268"
     CDS             13306..13542
                     /locus_tag="RO454_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDY0935060.1"
                     /translation="MVKLALQPRDSVACIAREHDINDNLLFKWLRLWLIKGRISRRLP
                     VTNSSGIGVELLPVEMTLDERPYPVFTRGNYEDQ"
     gene            complement(13742..14617)
                     /locus_tag="RO454_003269"
                     /pseudo
     CDS             complement(13742..14617)
                     /locus_tag="RO454_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(14896..15146)
                     /locus_tag="RO454_003270"
                     /pseudo
     CDS             complement(14896..15146)
                     /locus_tag="RO454_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15234..16478)
                     /locus_tag="RO454_003271"
     CDS             complement(15234..16478)
                     /locus_tag="RO454_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935061.1"
                     /translation="MLQIKARTKILALFCAMSFLMYVDRVNLSATAGLIKDELGLSNT
                     ELGVIFSAFAYTYAVFQIIGGWFVDRLGSRRMIIFCGIIWVIATMATGFVGGFISLFL
                     ARLLLGVGEGAALPSQARAITYWFKPQQRGFVQGVTHSFSRLGNAITPPIIVALVAFW
                     SWRGAFISLAIVTFIWLISWIISYKDDPRDHSGMTEEELEGVPIFEKKDLKVKAEPTP
                     WRALIKRMGPTMGVYFCYGWTSWLFFTWLPTFFMHGRGMDLKSSALFTAGVFLSGVVG
                     NTVGGVISDKVLKMTGNLVAARRNVILFSFISVLFLLVPVINTDSLLIMTICLSVAFF
                     CLELTIGPIWAVPMDITPKYVGIASGLMNAGSAVAGIISPIVFGIIIDKTGNWSLPFY
                     GSVALLIIGIFLTFFMRPDKSL"
BASE COUNT         4310 a         3851 c         3895 g         4493 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcaac attccaccat gcattccgat
       61 taacgccgca tagccagttg aactttgcta ctttgtgaga ggtagtacct tctatccagt
      121 gcgaatttaa ttaatggaat aaatgattat gagtgaaaat gatacaatcc caaagaagtc
      181 tacaagtcag attaacaaag cggtattctt tacatctgct ttgctaattt tccttcttgt
      241 cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat tttaaactac ttcagcaaca
      301 aatcttcacg aatgccagct ggttctacat ccttgctgtg gccctgattt tactgagtgt
      361 cacgttcctt ggactctcac gctacggtga tatcaagctg ggcccggacc atgcgcagcc
      421 tgatttcagc taccactcct ggtttgcgat gcttttttcg gcagggatgg ggatcggcct
      481 gatgttcttt ggcgttgccg aacctgtaat gcattatctt tcgccacccg ttggcactcc
      541 agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc tttttccact ggggactgca
      601 cgcatgggca atttatgcca ttgtggcgct gattctggcg ttcttcagtt accgtcacgg
      661 tctgccttta actctgcgct ccgcactcta tcccattatt ggcgatcgca tatacggacc
      721 tgtaggacat gcggttgata ttttcgctgt tataggcacg gtctttggcg ttgcgacatc
      781 actgggttac ggtgttttgc aggtgaatgc cggtttgaac catcttttcg gggtgcccat
      841 caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg gggttagcga cgatttcagt
      901 ggtgtccggt ctggataagg gaatacgtat cctgtctgaa ctcaatctgg gtctggcttt
      961 gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg cttctgctga agtcatttgt
     1021 ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa acgttcaacc tttacgcgta
     1081 tgagcccaag tcgagcaact ggctgggggg ctggacatta ctgtactggg gatggtggct
     1141 ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc tcccgcgggc gaaccattcg
     1201 cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt acgctaatgt ggatgacggt
     1261 gtttggtaac agcgcgatct atctcattat gaaccagggg gccacaggcc tcgccaatac
     1321 tgttcagcag gatgtggcgc tggccctgtt taatttcctg gagcatttcc cgttctcttc
     1381 tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc tttgtaacgt ctgctgattc
     1441 gggggcaatg gttgtggata ctctggcatc aggtggagtg gcaaacacac ccgtctggca
     1501 gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt gcgcttctcc ttgccggagg
     1561 gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg cccttctcag tgatcttact
     1621 aatatctata tacggacttt taaaagcttt gcgccgggat ttgaccaagc gtgaaagcct
     1681 gagcatggcg acaattgctc ctacggctgc acgtaaccca attccttggc agagaaggtt
     1741 acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa cgttttatgg acgacgttat
     1801 ccagcccgcc atgacgctgg ttcaggagga actgaacaag caggggacga taagccacat
     1861 tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat ttgggcaacg agctgaattt
     1921 tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc ttcgcgctcg ccgcaatgga
     1981 taatgatgag cagcagactg aacaacatcg atattatcgc gctgaggttt atctcaaaga
     2041 aggcggtcaa aattatgatg tgatgggctg gaaccaggaa cagctgatta atgacatact
     2101 ggaccagtac gaaaaacacc tgcacttcct gcacctggtt cgttaatagc aacatgccgt
     2161 cctgggggcg gcaattatta tcccggccgc aatatgaggg aatgcagaat gatttcacgc
     2221 tggaaatgga tgctgaagca gacaattaaa aaactatggt tcagggcaac gttattcgca
     2281 attgtcgcga taataacggc ccttttatca attcttttta aatcaatgat acctgagtcg
     2341 gtttccgtga aggttggtgc ggaagcagtc gataacattc tgaacatact ggcatcgagt
     2401 atgctggcag tgaccacatt ttcgctgagt atcatggtca cagcctacgg ttcagccact
     2461 actaatgtga ctcccagagc tacgcgttta gttgttgaag acgtcaccac acaaaatgta
     2521 ctggccacct tcatcggttc ttttctcttc agtctggtag ggattattgc cctcaatatg
     2581 ggagcttatg gagaaagggg gagagtcatt ttattcattg tcacactggt tgtcattgcc
     2641 ttaatcctca tcacattgct tcgctggata cagcatttga cctctctggg gagggttggt
     2701 gagacaacgg cacaagtaga acaggcggcc atcgaaacat ttattgcgag agcaagaaat
     2761 ccctgtctcg gcggatatcc atggcttgag aacaatgaac agccgaaagg aacagttgca
     2821 gtttatccga agaagattgg ctatgttgaa tatattgata tggtgaaact tagcaagctg
     2881 ctgaccaatg atccccgtca tgtatacctc gtggcgcagc caggcagttt catacatccg
     2941 tccatgccag ttttgtacct gagtcagggc caggagtcgt caatcaacac cgatttactt
     3001 gagacgatta ttgtctcgga tgcacgttca tttgctcagg atcctcgatt ttgtcttagc
     3061 gtcatggccg aaatagcctg ccgggccctt tcccctgcag tgaacgatcc tggaaccgcc
     3121 attgatgtca ttggcagagg tgttcgtata ctttccactt acgcgcagaa taaatctgat
     3181 gaaatagaag tgaaatatcc ttcagtacat gttgcaccac ttcagaataa cgatctactg
     3241 gaagactttt tctcacctgt cgcgcgcgat ggtgccagta tgagggagat tcagataaga
     3301 gtccttaaag gactgtcgat gctgagtaaa ggttggcctg agatatttgc tgaagccgca
     3361 cataccctgg catttgaaac attagagcat gcaactcatg ctgaccatat agattctgat
     3421 agatatctaa taaaatcaac ttattataat ttatttagtg gcgaatattc taataaaaaa
     3481 acatagctgc agagcaagca attcggttgt tggcaagtca agggcaaagc attcgcgtga
     3541 gaatgggcgt atgctttgtg ccaaaagcaa acgttgctaa ttctgcactg ttcagcgatg
     3601 gcgcagtagc gacaggcact gccactgtcc agtcggcaaa agcgaacact ttgccactgc
     3661 ggggataaac tgattgaagc tctcgcgcaa aatcacttat tcttttgcgt taataaaatg
     3721 taggagatgg gtcatgacag gcgaaaatcg tggccgggga aggccgcgaa agacagaaag
     3781 tacctatgca gatactcgta atgatctgat ccgcagtggc cttgaactgc tgacgcagaa
     3841 tggttttctt gcgacgggag tcgatgccat tgttaaaaat gccaatgtcc ctaaaggctc
     3901 attttattac tactttaaga gtaaagagga ttatgctcag accgttctga acgcctatga
     3961 cagtttcttc gagcataaac tcaaaaaaca tctacatgaa tcatcctgca caccaatggt
     4021 acgtctggaa aatttcatca atgatgcctg tgaggggatt aaaaaataca attttacccg
     4081 cggctgcctg gttggaaata tgatgcagga gagtccgggg cttccccaat ccttcattaa
     4141 agtactacag aatattcttg agagctggca ggcacttgtt gcggcctgcc tgtccgacgc
     4201 gttatcttca ggcgaaataa gttcaaatat gaataataca caactggcgg ctattttctg
     4261 gagtggctgg gaaggtgcgg taatgcgttc caaactatat tgttctactg agccagtgta
     4321 cgacttctgg agctatttca aaacctcggt acgctatcag tcttctcagg aagcaacaac
     4381 tggccagtaa aaggggccgc agcccctaaa acttattata ctaagcctga gctcggcatc
     4441 catctaatta gctatattga atgactgcac gaccgcgaat tccgccactc agaagttttt
     4501 cagccacagt gatggcgtca ctgagcggat aaacggtagt gagactatcc attctggact
     4561 ctggcaaata ctataccgcc tgttgccaga ttgctgctct tttctctttc gcgaacatga
     4621 cgctatcaat ccctttcagc gtgacaccac gcagaataaa cggagccata ttgcccatca
     4681 aatccatccc ctgagccatg ccgcaggctg caacaatact gccatatcgg accccggcgc
     4741 aggcatttct ccagtaacag ggggttgtat tgtattgtat tttattgtgc cggttcaggt
     4801 gtgcgtttcc cggcgcgtct gcaccggctt aaccaaattc aacagggata aaatagtgca
     4861 aataaccgaa gtattacttg cagtaatcgg gggaagcatc cggggtttta ttcaacaggc
     4921 tgtagatcac tgactgcgtc tgttggccgt ccacttcacg cgtcattcga ccagagtaaa
     4981 tattgatgat tactagattc gtgtatttta tcaggcattt tacttgcgaa taaatcccct
     5041 tgctatccag cgaaaccaca caggtagcta ttaccatatg tggtaataca ctagttacag
     5101 gaaaaatatg caagttccgc aatacgttgt gtgttgctcc ttagccagga tcttttcttc
     5161 catgcgagat atgacaacaa ttttgaggaa ggatgttcgt aactggtaga gttactacaa
     5221 caagcatggg aggctctgac tctgacagac attgtactgt ggaatgttgt tacaggttgg
     5281 ggcggcaacc gtgccaggaa accatgagcc gttcacggca ttaaaggcca cacttcagtc
     5341 gtttacattc cctatggtgg caggcatctt ccattaaggc gtcagagcgt taccaacgtt
     5401 atgttgtcat ggctcatctt tcatagtcga tgcttatctg gtggctgaag ctagccgcag
     5461 ataaagagtg tgaagttgat accatggagt ggagtgacgt gcaggcagga gattcgctct
     5521 atgcagggtt gtgagatgaa ttcaaaccgg ctggcagcgc cagtgatatt cgaagattcg
     5581 tccggttgtt acccagtatg cattaaaaac ccatacatta tggacaatat aacggcttct
     5641 acaacaagga agtgcctgtt taccatccgg gtgtaatgac tcacctttgt gggcttttgc
     5701 ccgtcgcgct cttaacgttg aagacattct ctgcctgccg attcaggact gcgacaaaag
     5761 ccatatctgg ctactggtca aggatgacca gcgtctggag taggtgataa gccaaataga
     5821 taagctggaa tatgtggtga aagtgcaacg taatcagtcc aacccgacga tgtttaacaa
     5881 gattgtggtg ttttttcagt aagtcattcg tcttctgaat caggtgtaat gatctttaca
     5941 gaaccattct ggcagtgcgt aacataatct gccggtaacg gacgatcgct aatcagaacg
     6001 tcaaatgcag agatcggtgc gaggctggca gaggccactt catcgaacaa cgcgtggcgg
     6061 gcgagcaaaa ttttccgtag tccacggttc atcgctttac gcttagtggc aagatcttca
     6121 gggttaaacc agctgacgcc aaagtgatta tgcacaccgc tggcggaaat aaatattttt
     6181 cgcggattga gagagtctaa tggcgaagag ttactggcat cgtaaaaagc atcacttctg
     6241 gcacgatacg taccaccaca aagtattgct gttacattag gcttttcatt caacgcaaca
     6301 aagacgcgat gtgagtaaca gatgccggtg aaggtgattg catccggaat catgcttata
     6361 acgagtggta tctcctggcc attatcaaag aagatcagat cattttcatt aaccattccg
     6421 gcagccagaa ttgcaatagg taagtcatca cgatgatttt ttggaacgtc atggattact
     6481 ggcatggatg gcgcgggttt attcaccatt acaatatagc cacccagtag ggtcagtggc
     6541 agaggttcat cttcctgatg gagatcgcga cgaatagtca ttacagaaac ttccagcatt
     6601 cgcgctgcgt ctttcaaatg gattctgtcg gttttcttaa gcagttccat caaacgtcgg
     6661 atacgctctt tttgcttcgt ttccatcaac tctctctccg taagaatgtt ataaaaataa
     6721 cattttggcg ttagtaaaca ttgttttcat taccatacta tacgaccgcc cctttgaaat
     6781 gaaaagggta taggctgctg agttttgggc actaatcttg ttaggttgct tatgattaga
     6841 tagtgttggc ctgagtgatg agactggaag tctattaaat aaacatcggg ttgatgtgct
     6901 aatcgcgatc tcagtcacaa atgatgcaaa agtaacatgc taatgttatt taaataccat
     6961 tgttatgcga aagtttataa gaggtagtga aatggacatt gcagttattg gttccaatat
     7021 ggtggatctc attacttaca ctaatcagat gcctaaagaa ggagaaactc tggaagcacc
     7081 tgccttcaaa atcggatgtg gtgggaaagg tgctaatcaa gcggtggcgg cggctaaatt
     7141 gaattcaaaa gtcctgatgc tgactaaagt aggtgatgat atttttgcgg ataatactat
     7201 tcgtaatctt gaatcatggg gaattaatac cacttatgtt gagaaggtcc cctgtaccag
     7261 cagtggtgtt gcaccgatct ttgttaatgc caactcgagc aatagtatat taataattaa
     7321 gggagcgaat aaatttcttt ccccggaaga tatcgatcga gctgcagaag atctgaaaaa
     7381 atgcaaattg atcgttttac aactggaagt tcaactggaa acagtttacc acgcgattga
     7441 gtttggtaag aaaaatggta ttgaagtgtt attaaatccg gctccggcat tgcgtgagtt
     7501 agatatgtct tatgcctgca aatgtgattt ctttatcccg aatgaaacag aactggaaat
     7561 attaaccggt atgtcggttg atacttatga tcatattcgt ttggccgcac gttcgttagt
     7621 tgataaaggg ctgaacaata ttattgttac catgagcgaa aaaggcgcat tatggatgac
     7681 ccgcgatcag gaagtccatg ttcctgcatt caaagtaaat gctgttgata ctagtggcgc
     7741 tggtgacgca tttattggtt gcttttccca ttattatgtt caaagcggtg atgttgaagc
     7801 cgctttgaaa aaagccgctc tctttgccgc tttcagcgtc acagggaaag gtacgcaatc
     7861 atcttatccg agtattgaac aatttaatga atttttaacc ttaaacgaat aatcatcctg
     7921 cacagttaaa ggtagcacta tgaacgataa aaacatcatt cagatgcctg atgggtatct
     7981 gaataagact cccttgttcc agtttatttt gttatcgtgt ttattcccgc tatggggatg
     8041 cgcagctgca ttaaatgaca tattaatcac tcaattcaaa agtgtatttt cgctaagtaa
     8101 ctttgcttca gcattggtgc agagcgcatt ttatggaggt tattttttaa ttgcaatacc
     8161 agcatccctt gtgattaaaa agaccagtta taaggtggca attttaattg gcctgacgct
     8221 gtatattggt ggttgtacac tttttttccc tgcctcgcat atggcaacct acactatgtt
     8281 ccttgcagct atttttgcga ttgcgattgg cctgagtttc cttgaaactg cagcgaacac
     8341 atacagttca atgatcggtc caaaagccta tgccacattg cggctcaata tcagccagac
     8401 cttttatccg attggtgctg cgtctggtat tttattgggc aaatatctgg ttttctccga
     8461 gggggaaagc cttgagaagc agatgtccgg aatgaacgcc gagcaaattc acaactttaa
     8521 ggtgctgatg ttagagaaca cccttgaacc gtataagtat atgattatga ttctggttgt
     8581 ggtcatggtg ttgttccttt taacccgctt cccaacctgt aaagtggcac agacaagtca
     8641 ccataaacgc ccgtcagcaa tggatacgct gcgctatctg gctaaaaatt cgcgctttcg
     8701 tcgtggtatt gtggctcaat tcctttatgt gggtatgcag gtcgcagtat ggtcatttac
     8761 catccgtctg gcactggagt tgggcgatat caacgaacgt gatgcttcaa acttcatggt
     8821 ctacagcttt gcctgcttct ttattggcaa gtttatagcc aacatcctta tgacacgttt
     8881 taatccggaa aaagtcctaa ttctctattc cgttataggg gcgctgtttc tggcatatgt
     8941 tgcactggct ccaagtttca gcgcggtcta tgtcgccgtc ctggtaagtg ttttgtttgg
     9001 cccttgctgg gcgacgatct atgcaggaac actggataca gttgataatc aacatacaga
     9061 aatggcagga gcagtcattg ttatggctat tgttggcgca gcggtggttc cggcaattca
     9121 ggggtatatt gcagatatgt tccactcact acagctttca ttcctggttt ctatgctctg
     9181 ttttgtctat gtcggtgtct acttctggcg tgaaagcaaa gtacgtaatg cactggctga
     9241 agtcacagag tcctgaggag aatcgttatg agtacgcgta tttatttgtg gcgggcgctt
     9301 tttggcgaaa aacctcgtat cttgctggaa aatagtgatt ttactgtcac gtcttttcgc
     9361 tatgacagtg gtgtcgaggg gcttaaaatt gcaaacagtc gtggacattt aattattctt
     9421 ccctggatgg ggcagatgat ttgggatgcg caatttgatg gacacagtct caccatgtgc
     9481 aatatgtttc gccagcctaa accggcgaca gaggtgatag aaacctatgg ctgttttgcc
     9541 tttcattcag gtttgctggc taatggctgc ccatctgcgg aagatactca tctgctacac
     9601 ggtgaaatgg cctgtgccgc gatggatgaa gcatggatgg aattagaggg agatatgtta
     9661 cgccttaccg ggcgttacga gtatgtaatg gggttcgggc atcattatct ggctcagcca
     9721 acagtggtcc ttcacagatc ctctacattg ttcgatatta agatggccgt aaccaatctg
     9781 gcttctgtag atatgccact acaatatatg tgccacatga attatgcata tataccaaat
     9841 gctacgttca gccagaatat accggacgaa atattaaggt tgcgtgaatc agtaccttcg
     9901 catgttaatc ccaccgctca gtggctggcg tttaatcaac ggattatgca gggagaagcc
     9961 tctctgtcga cgctgaatca accggaattt tatgatccag aaatagtctt ctttgcagac
    10021 aaactggacg cttacacaga tctgccagag tttcgcatga tcgctcccga tggcactacg
    10081 tttgttactc gtttctccag tgccgaacta aattatgtga cacgctggat cctttataac
    10141 ggcgagcaac aggtagccgc ctttgcatta cctgcaacct gtcggcctga ggggtatctt
    10201 gccgcgcaac gtaatggcac gctgattcag gttgcaccgc agcaaacacg cacttttaca
    10261 gtcaccacag gaattgaata accacttaag gcctgaacag cggcgcgctt atcgttaagg
    10321 taagcgcgtt tttttacccg ccaggacaag actatgatag cgttgccctt atagacgatc
    10381 acattatcgt ccactccaaa tttgcgcaac tactgtagag tggtgtactc cacagcttct
    10441 tttaggatct caacttccat tgttttctta ccgagcaagc actgcaactc ttttatttat
    10501 ttgtagtggt caacaaaaac tgtaagcgtc aacggagcac cgtattgacg cttatttatt
    10561 ggtgagtact acgttccatg gcaggagttc gtcaactcgg ttggaaggcc attccggcag
    10621 tacgctcagg atatggcgca gatacgcttc cggatcgata ccgttcagac ggcaggtgcc
    10681 gatcagcccg tacaacagtg ctccacgctc gccgccgtga tcgctgccaa agaacataaa
    10741 gtttttcttt ccgagacaga ctgcacgaag cgctctttcc gcagcattat tatccgcctc
    10801 cgccagaccg tcatcactgt aataacagag ggcatcccac tgattcagta catagctgaa
    10861 cgcttcgccc agtctggatt ttttcgacag cgtaccattc ttctccacca tccattcatg
    10921 cagcgacgtc agtaacactt tgcttcgctg ctgcctgacg gcaagacgct ctgactccgg
    10981 taatccccgt atttcatcct cgatggcgta cagttcactg attcgcttca gggcttcttc
    11041 tgccgtcgca cttttgctgc tgatgtatac atcgtggatt tttcgccggg catgggccca
    11101 gcacgcaact tctgtcagtg caccaccttc acgttctgca ctgaacaacc tgtcgtaacc
    11161 tgtgaacgca tccgcctgca ggataccccg gaaggggcgg aggtgttgct ccgggtgttt
    11221 cccctgccgg ttcggcgagt acgcgaacca gaccgctgga ggagatgacg aacccacatt
    11281 gcgatcatcc cggacatacg tccagatacg ccctgttttc gcctttttct gacccggtgc
    11341 cagtaccttt accggtgtgt catcagtgtg aaccttgcgg gtgttcatta cataacggta
    11401 cagggcatca ttcaccggtg tcattaactg gcagcacgcg tcaacccagt tggagagtaa
    11461 ggcccggctc agttcgacac cctggcgggc aaagatttca ctctgacgat acagtggcag
    11521 atgttcgcag tattttcccg ttaacacgcg ggcaagtaat ccggggcccg cgataccacg
    11581 ctctatcggg cgggacggcg ccggtgcttc aacaatacag tcacattttg tacaggcttt
    11641 ttttacccgt tctgtgcgga tcactttcag ggcactgctc accagttcca gctgttcagc
    11701 gctgacttcc cccagataat ccagctcacc gccacactcc gggcaacagc tttcttctgg
    11761 ctccaggcgg tgtatttcac ggggaaggtg tgccggtaac ggacgacgat ggcgcgactg
    11821 tcgcaactgg cggggaacct gaggatcgtc ttcccgccca ctgtaacgat cgctgtcctg
    11881 ttcacgttgt ttcagcagag cctcagccag ttcaacttca cgacgcagtt tttcagaacg
    11941 ggtaccgaac agcatccggc gcagtttttc tatctgagcc cgcagatgtt ctatttcccg
    12001 ttcatcttct tcgatctttt cttcggcacg tgtcagtgca gagcgcagga aggcttccgt
    12061 ctcttcaacc agactcagtt gctggtcttt ctgacggagg gcttcagcct gctcagagag
    12121 caacctttcc agctctgcga tgcgaatgag gtatttctga ctcatgaccg tttttataat
    12181 gcggtcagga gttttttaca acattgtcag tgagttacgg ctggatgttt ttggctgacg
    12241 ccagtccagc ttatcgagga gcattgccag ttgcgagcgg gtaatggata ccttgccgtc
    12301 acgtaccgca ggccagataa actggccttc ctccaggcgt ttggtgaaca ggcacagacc
    12361 atcagcatca gcccaaagaa ttttgacggt gtcaccccgt cggccacgga agataaacag
    12421 gtgaccggag aagggattat cattcagcac atgttgtacc tgttctccca gtccgttgaa
    12481 ggatttacgc atatcggtaa cgccggcaac gagccagata cgggtacctg atgggagtga
    12541 gatcatcttc ccctcccggt cagttcacgg atcaacactg tgagcagctc tggcgatgga
    12601 ttttccagcg tcatgttacc gtgacggaat tccaccttgc aggaactggc actgactctg
    12661 gtctgagtgg aagtggataa agacggcgca atggccgcca caggttcttt ctgctcatcc
    12721 ggcgttattt ctacaggtaa taattcaacg ccagtgtcag aagaggtcgt taccggaaga
    12781 cgccgcgaaa cacgcccttc gttctgccag agcctgagcc atttgaaaat aacattatca
    12841 ttgacgccat tttcacgtgc aatctgtgca acacaagctc caggttgtga tgccagttcc
    12901 accatacgaa gtttgaattc attcgaatag tttttacgag gttcttttcg ccagtcctgt
    12961 aattccatac ttagatgtcc gtctatatca gatgggcgtc taagttacca attctcgtct
    13021 gatggctaca tacggcggtc agtttacgct taccaaaaac tggtcacaat ttcagatttt
    13081 ttccagtatc tattttccga ttcgttcggc ggtaatccac cgttatattc atgaggccgg
    13141 agtgcactat aaaagtgtat gcgtaaaccg actgctgtat gtagccatta agcctgtatt
    13201 ggtaacatag gtacccacct tttcaacaag tggacacctg ttatagaaca aaaaacatta
    13261 tcagcagaac cccgcaggtc attttcaaat gagtttaaac ttcaaatggt taaactggct
    13321 ttacaaccaa gggattctgt tgcctgtatt gcccgggagc acgatatcaa tgataatctt
    13381 ctgttcaaat ggctcaggct ctggctgatc aaagggcgta tatcacggcg tcttccggta
    13441 acgaactctt ctggcattgg cgttgaatta ttacctgtgg agatgacgct ggatgagcgg
    13501 ccttaccccg tttttacgcg aggtaattat gaagatcaat gactagccgt cgaaccgtgt
    13561 gcatgaactg ctgtactgaa acttctcagc tgtaaaataa tccttacgct acgtacttct
    13621 cggagcgctt acgattaatc agatgcagtc ctaactggaa gaaagaaaaa tggagaaaat
    13681 aagaggaatt aatatcctcc acgttcctga aatcctggag gatattaatg tcatcaaaat
    13741 attagcgaac gataatccct aacaaaatac ccagtgcgcc aaagtcagca tcactgaagg
    13801 tggtgttagc aaaaccaata tcacccagca caggcaataa gaatactgac aggaaggtaa
    13861 taagcaagcc ttgcgcaaag gcacccaaaa tcgcaccacg acgtccaccc gttgcattac
    13921 cgaatacccc tgcagctgca cctacgaaga agtgcggcac cacaccagga ataatcacga
    13981 tcatgttcag cagatacaac gtgaacatac cgattaaacc tgcagcaaag ctactcagga
    14041 aaccaaccag tacagcgtta ggtgcgtaag ggaagatgac cgggcaatcg agagcaggtc
    14101 tggcattcgg aaccagcttg tctgaaatac ctttgaatgc cgggacaatc tccgcaatca
    14161 ccatgcgcac accctgcaga atgatgtaca cgccagctgc gaaggtaatg gattgcatga
    14221 tagagaacat gaaccagttt ttaccaccgc taagctcttt caccgcatct gcacccgcaa
    14281 acaggcacgt cagcaggaaa attatgatca tggtaaaaga gatagcaacc ggcgtgtcgc
    14341 gcaagaacag cagacttttc ggcacattca tgtcttcagt cgagtgctct ttattaccaa
    14401 acttgctgcc gatgaaaccg gccaatacat aagataatgt tgagaagtgg ccgatggcaa
    14461 cgtcatcaga accggtcact ttcttcatgt acggatgagc aatagccggg aaaaagacca
    14521 ttgccacgcc aacaaccaaa gagccaacgg ctataagcgt aatgccggtc atgcctgctg
    14581 ttgccagaat taccgctacc atcatcgaca taaacaacgc ccgcagcatg agaatggaca
    14641 atagacattc gggaggattt tttcatataa atctccttgc tcatttttaa tgaacgaagg
    14701 aggagacgac ggagaaatca ccttcctgaa cgggatcaag ctaccactgt cggatccgcc
    14761 acactccctg aaccatgttt tttaagggtc ataccaccaa aaggtcgacg gctgcttcct
    14821 tcgctatatt agactgccag ttttaccggg gagtttctga gactcgggac ggggtttcac
    14881 acatggaaag ttttttaata tttcgttttc catttcaatt cgttgtagct ttttctttag
    14941 ctcacgtagt ttgatttgtt ccggtgttat cagggaggct tttggcgttt accctgacgc
    15001 tcatcacgca gttgtttgaa ccattccgtc atcgtggaaa gaccaacatc catagcctta
    15061 gcagcgtcag aaacagtgta attttgatcg acaaccaact gagcggattc acgtttgaat
    15121 tcaggactga aatttcttct tttcataggt gcacctgtgt tgttctaagg tgagcatatc
    15181 accctgttca gatggccaaa ttcagtagac cacttcagcc ctgaattcga cttttacaat
    15241 gacttatctg gacgcatgaa gaacgtcaga aatataccaa taataagcaa tgcgacggaa
    15301 ccgtagaatg gtaaactcca gttgcctgtt ttgtcgataa tgatgccaaa gacgataggt
    15361 gaaataatgc cggcgacagc ggaaccggcg ttcatcaatc cgctggcaat tcccacatac
    15421 ttcggcgtaa tatccatcgg gacggcccag ataggcccta tggtcaactc aagacagaag
    15481 aacgccacac tcagacatat agtcataatg agcaacgagt cagtatttat aaccgggacg
    15541 agcagaaata gtacggagat aaagctgaaa agaatgacgt tacgccgggc tgctactaga
    15601 ttgccagtca tcttcagcac tttgtcgctg attacgccac caaccgtatt acctactaca
    15661 ccggacagaa atacgccggc agtaaagagc gccgatgact taagatccat gccgcgacca
    15721 tgcataaaga aagttggtaa ccaagtaaaa aacaaccaac ttgtccatcc gtaacagaaa
    15781 taaactccca ttgtcggtcc cattcgtttt atcagggctc tccatggagt aggctcagct
    15841 ttaactttta gatctttttt ctcgaaaatt ggtacgcctt ctagttcttc ttcggtcata
    15901 ccgctgtggt ctcgtgggtc atctttatac gatatgatcc aagatattaa ccagataaat
    15961 gtgacaatag ccaaactgat aaatgcgcca cgccaagacc aaaatgccac taatgcaaca
    16021 ataatggggg gagtaatcgc atttcccaaa cgagagaatg agtgcgtaac gccctgaaca
    16081 aagccacgct gctgcggctt gaaccagtag gtgatcgctc gcgcctgaga aggcagcgcc
    16141 gcaccttccc ctacacctaa tagcaatctg gcgaggaata gtgatataaa gccaccgacg
    16201 aaaccagtcg ccatggttgc tataacccaa ataatgccac aaaaaatgat catgcggcgt
    16261 gagccaagtc gatcaacgaa ccagccaccg atgatttgaa aaactgcata ggtataagca
    16321 aaagcagaaa aaataacgcc gagttctgta ttactcagtc cgagttcatc tttaatcaac
    16381 cccgcagtag ctgataagtt tactcggtcc acatacatta ggaatgacat tgcgcaaaat
    16441 agcgcaagta tcttggttct ggcttttatt tgtaacattt ttgtctccta aactagcgtt
    16501 aaattgctag taaataatgt caattagtta catatattag caacgcccc
//
LOCUS       DAPQZD010000106        16522 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPQZD010000106 DAPQZD010000000
VERSION     DAPQZD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.106"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            121..1593
                     /locus_tag="RO454_003272"
     CDS             121..1593
                     /locus_tag="RO454_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDY0935062.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDVVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            1590..2606
                     /locus_tag="RO454_003273"
     CDS             1590..2606
                     /locus_tag="RO454_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDY0935063.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREKKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            2617..3627
                     /locus_tag="RO454_003274"
     CDS             2617..3627
                     /locus_tag="RO454_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HDY0935064.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDADTAQQVTDVLIHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LALEEGLGFVSVKNTSHCGALSYFAEMVTNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKDGYGTTDPHKIE
                     NLLPFGQHKGSGIALAIDALTGMLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAQMVTELHAVKPAPGIEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            3700..4683
                     /locus_tag="RO454_003275"
     CDS             3700..4683
                     /locus_tag="RO454_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDY0935065.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILKSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYSDM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            4725..5207
                     /locus_tag="RO454_003276"
     CDS             4725..5207
                     /locus_tag="RO454_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDY0935066.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNFH
                     "
     gene            5218..6522
                     /locus_tag="RO454_003277"
     CDS             5218..6522
                     /locus_tag="RO454_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDY0935067.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(6593..8005)
                     /gene="ftsP"
                     /locus_tag="RO454_003278"
     CDS             complement(6593..8005)
                     /gene="ftsP"
                     /locus_tag="RO454_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDY0935068.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(8080..8817)
                     /gene="plsC"
                     /locus_tag="RO454_003279"
                     /gene_synonym="parF"
     CDS             complement(8080..8817)
                     /gene="plsC"
                     /locus_tag="RO454_003279"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDY0935069.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(9052..11310)
                     /gene="parC"
                     /locus_tag="RO454_003280"
     CDS             complement(9052..11310)
                     /gene="parC"
                     /locus_tag="RO454_003280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDY0935070.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYVAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11448..13055)
                     /locus_tag="RO454_003281"
     CDS             complement(11448..13055)
                     /locus_tag="RO454_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0935071.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(13164..13646)
                     /locus_tag="RO454_003282"
     CDS             complement(13164..13646)
                     /locus_tag="RO454_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDY0935072.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPDAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            complement(13699..14091)
                     /gene="ygiW"
                     /locus_tag="RO454_003283"
     CDS             complement(13699..14091)
                     /gene="ygiW"
                     /locus_tag="RO454_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDY0935073.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            14243..14902
                     /gene="qseB"
                     /locus_tag="RO454_003284"
     CDS             14243..14902
                     /gene="qseB"
                     /locus_tag="RO454_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDY0935074.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14899..16248
                     /gene="qseC"
                     /locus_tag="RO454_003285"
     CDS             14899..16248
                     /gene="qseC"
                     /locus_tag="RO454_003285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDY0935075.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAKQGGFEAKVSW"
     gene            complement(16294..>16522)
                     /locus_tag="RO454_003286"
     CDS             complement(16294..>16522)
                     /locus_tag="RO454_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDY0935076.1"
                     /translation="LESGDNICSIPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMR
                     EMFEFWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT         4134 a         4130 c         4059 g         4199 t
ORIGIN      
        1 cgccttgttg ttctgtttcg tacttttacc tttctctgca agcgtgatgc atctctctgt
       61 ttttggtcta gtgaattatg taaattggtc tacacacaag tcgtcaggag taagagcata
      121 atgtcaggga acgaaagtat cgctgccgcc cactatgatc aggtaaccac ctggcctcgg
      181 gacgggttac aggcagatat tgtccatatt ggttttggcg cttttcatcg cggacaccag
      241 gctgtctaca cggatctcac taatcaactt tcggacaccc gctgggggat ctttgagatc
      301 aacctgtttg gtgatgctca actgatcgaa aacttaaatg cgcaaaatgg gctgttttcg
      361 gttgtggaaa catctgcatc gcaatctacc tcacgccttg tgcgttcggt ggctggcggt
      421 attcataccc ccagagatgg cattgccgca gcaatccata aactggctga acctcaggta
      481 aaaatcgttt cattaaccat caccgagaaa ggttattgcc tcgatccgca aacacgttca
      541 cttgatctca ccaatggatt aatccaacac gatctacaaa acccggatgc gcctctgtca
      601 gctattggcg tgatcgtctg tgctttgcaa caacgtaaag cggcaggact cgccgctttt
      661 agtgtgctct cctgtgacaa cttgccagac aatgggcatc tgacgcgcaa tgccgttctc
      721 ggttttgccc gacaactgga ccagcccctg gctcagtgga tcgaagaaaa tgtctcattt
      781 ccaggcacga tggtagatcg cattgttccg gcaatgactg aatcgcaatt cgccttactg
      841 gaaacgaaaa ccggttatgc cgatccctgc gggatcgtct gcgaatcatt tcgtcagtgg
      901 gtgatcgaag ataattttgt gcgcggacga ccggaatggg ataaagccgg cgcgatgttt
      961 gtcagcaatg ttcagcctta tgaagagatg aagttacgca tgttaaatgg tagccattca
     1021 tttctggctt ataacggctc tttggcaggc tatgagttta tctggcaatg catggaagac
     1081 gctaattttc gttccattac ccaccaactg atgattaatg aacaagcccg aacacttaat
     1141 ccagacttaa atatcaacat ccaggaatac gccgacctgt taattgaacg ctttagcaac
     1201 cgtaacgttg cgcaccgtac cgggcaaata gccatggacg gttcacaaaa gcttccccag
     1261 cgtgcactga cgccctggct gaaattacat cagcaaaagc aaaacaatgc tgttctgtca
     1321 ctgctcgttg ctggttggtt gcattatgtc attgatgttg ttgagaaaag ccagtctgtc
     1381 gctgatccaa tgaatgacca atttcaggcg ctaataaagg aacaacaaga cgcatggcaa
     1441 caggcgctcg cattactgca ccttagcgcc atatttggtg atttaagcaa ccatcagcca
     1501 tttataaatg aaataaaaat cgcctttgca aatataaaaa acaaaggcat caaggccacc
     1561 atcagccaat tattatcgga tgagcagaaa tgaaaacatt aatttgtcag cagcctggcg
     1621 ttatggaata tgtggaaaag gatattccca caccagcaga taatgaagtg ctgttaaaaa
     1681 tcaaagctgt gggtatttgt ggtactgata ttcacgcttt tgccggcaga cagccttttt
     1741 ttagctaccc acgtgtatta ggtcatgaaa tatgcgccga agcggtttcg cgaggcagcc
     1801 agtgccaaac agcacaatca ggccagcgct attccgtcat cccatgcatt ccatgtggcg
     1861 agtgcgcagc ctgtcgggaa aagaaaacga actgctgcga acgtgtttcg ctgtatggcg
     1921 tgcatcagga tgggggcttt agtgagtacc ttgcggtacg tgaagacaac cttgtgcctc
     1981 tccctgacga ggtcagcgac agtgccggag cattggttga atgtttcgcc attggtgcac
     2041 atgccgttcg tcgggcagag atcaaggctg aacaaaacgt actggtgatt ggcgctgggc
     2101 caatcggtct ggctaccgca gccatcgcca gggctaaagg ggcgcatgtt gttgttgctg
     2161 atattgactg tcaacgtcgc cagcacgttg tggatcatct ggcaattaat gtcttcgacc
     2221 caacacagga aggttttatt gccgcgctta gtgaagtatt tggcggcgaa ctggcttgcg
     2281 tagtcctgga tgcgacggga aataaagctt caatgagtca tgatgtaaat cttattcgtc
     2341 atggcggcaa aattgttttc atcggtttgt acattggtga acttgttatt gacgatccga
     2401 ccttccataa aaaagagaca acgttactca gcagccgcaa tgccacacgg gaagattttg
     2461 cgttggtgat tgaactgatg cgcagcaata aaattcacga aaatttaatg aaaaaccagg
     2521 cgttcaattt ctttagtgtt ggcgaagatt accagcgtaa cgttgtagaa aataaaaata
     2581 tggtcaaggg tgtgatcact ttttaaggga acatatatga caactgttta tgtcagtgaa
     2641 gaaaatttaa aatcactggt gcaccacaaa ttacataccg ccgggctgga tgccgacact
     2701 gcacaacagg tgacagatgt tttaatacat gccgatatta caggtgtgca ttcacatgga
     2761 gttatgcgtg tcgaacatta ttgtacccgc cttgctgccg gagggttaaa taaagccccg
     2821 cagtttagta ttgagcaaat ttcaccgtca gtggccattc tcgactctga cgacggaatg
     2881 gggcattcgg cattaattag cgccactgag cacgctatta aactggccct ggaagagggt
     2941 ctcggttttg ttagcgttaa aaatacgtct cactgtggcg cgttatctta ttttgcagag
     3001 atggtcacca acaaagggtt ggttgctatc gtaatgacgc aaaccgatac ctgcgtggct
     3061 ccccatggcg gcgccgagcg ctttttgggt actaacccca tcgcctttgg tttcccggtg
     3121 gaaaacagcc atccgatgat tgttgatatg gcaaccagtg ccacggcttt tggcaaaata
     3181 cttcatgcaa aggaaacagg aaaacatatt ggtgaagggc tggcgataga taaagacggt
     3241 tatggcacca ctgatccgca taagattgaa aacctgctac ctttcggcca acacaaaggt
     3301 tcaggcattg cgctggctat tgatgcactg actggcatgc tgatgaatgc gaattttggc
     3361 aaccatattg ttcgcatgta tggtgattat gacaagatgc gtaagctggc aagtttggtt
     3421 attgccatcg atccgaaaaa gctcggcaat cctgtttttg caaaaaccat ggcgcaaatg
     3481 gtcacggagt tgcatgccgt taaaccggct cccggtatcg aaaaagtgtt agcgccgaac
     3541 gatccgcaaa tgcactacaa agaaaaatgc caacaggaag gtattccggt tccagcagga
     3601 atattccatt atctggcaga gaattaatca ctacttttta ttataacgat agaaaagcgc
     3661 tgtatttatc gtttctgtta tattaaccag gagcaggata tgaaagcact tcgtcctctg
     3721 acggcatcgc ttatgctgtt aaccagttgt ttattaatat ctaataccac actggcaaaa
     3781 acaacattaa aactgagtca taatcaggat aaaagccatg ctgtccataa agcattaagc
     3841 tatttagcgg ataaaaccaa agagtattct aatggtgaat tagttattcg catttaccca
     3901 aatgcgacat taggcaatga gcgtgaatca ctggaattaa tgaactccgg tgcattacag
     3961 atggttaagg ttaatgctgc atcactagaa tcatttgccc cggattacag ccttttcagt
     4021 ctgccgttct tattccgcga ccgtgatcac tattatcgtg ttctgcaaag tgatttaggt
     4081 aaaaaaatac ttaagtcatc agaaagcaaa ggttttgttg gtattacgta ctatgacggc
     4141 ggtgcgagaa gtttttattc caataaaccg attacaaaac ccgaagattt agcgggaatg
     4201 aaaatcaggg ttcaacaaag ccccagcgcc attgcaatga tgaaagcact cggtggtgtc
     4261 gctaccccga tggcgcaagg cgaactctac accgcacttc agcaaggagt ggttgatggc
     4321 ggagagaaca ataccgttgt ctattccgat atgcgtcacg ccgaagtcgc aaaagtctat
     4381 tcacgtgatg aacacaccat ggtacctgat gtgttaatta tcagcaccga cgtgttgaat
     4441 aaacttggtg ataaagagcg cacggcatta ttaaaagcag ccgatgagtc aatgatgcag
     4501 atgaaggacg tcatctggcc tgcggctgaa aaagaagcct acgacaaaat gaaagggatg
     4561 aacgcaacag ttgttgatgt tgataaatcc gctttcaaag aacgcgtcaa accgttatac
     4621 gatgaattca aggcgaaaga tgcacaatca gccaaaaacc ttgagctagt agaaagtatg
     4681 taaccctaat gggcaggtaa ttcctgcctt ttcatggaag acttatgcga acactcactc
     4741 atattcttaa taagatcctt gccggatgtt gctgcattat tctcgccatt atggtgttct
     4801 gcgtaacctg gcaagttatt gcacgtttta tttttaacgc tcccagcact gtgcttgatg
     4861 agttcaccca gattttattt atgtggatga ttttacttgg cggtgtatat actgcggggt
     4921 taaaaaaaca tctggccatt gatttgttgg ctcaaaaact tccggctgca tctgttctga
     4981 cgcttgacag ttttatccaa attattatta cggtctttgc ggttattttc atgatctatg
     5041 gcgggaatat tgttgttgaa aaagcacaac atgttggaca aatctctcca gtattaaaat
     5101 ggccaatgga taaagtatat tgggttatgc ctgtcagtgg gcttattctg gtctggtatt
     5161 cagtaatgaa tattattgac aactatcgta aacggaattt tcattaacga gggaaatatg
     5221 gactttgaat atatctaccc cgtcttaatt ttatttggca gttttgccgt catgctggca
     5281 ataggtgtgc caattacttt tgcgattggt ctttcttcgc tgttatctat tattactgcc
     5341 ttaccacccg atgccgccat ttctgtgatt tcgcaaaaga tgactgtggg gctggatggc
     5401 tttacgctat tagccattcc cttcttcgtg ttagccggaa acattatgaa taccggtggt
     5461 atagccagac gactggttaa cctggcgcaa gcattagttg ggcgtcttcc tggctcactg
     5521 gctcattgta atatcctcgc gaatacactg tttggtgcga tttcaggttc agccgttgcg
     5581 tcagccgccg cggtaggtgg gattatgtca ccactgcaag aaaaagaggg atatgatccg
     5641 gcgttttcag cagcggttaa tattgcctct gcccccattg gcctgatgat cccaccgagc
     5701 aatgtgttga ttgtttattc ccttgccagt ggcgggactt ctgtcgctgc gttgttctta
     5761 gctggatatc tgccgggcat cctcaccgct gttgccttaa tgtttgtagc tgcactttat
     5821 gcccgacgtc accattatcc ggtggccgag cgcattaatt accgtcagtt tttacaggta
     5881 ttccgcgaat caattcctag cctgatgctt atttttatca ttattggcgg tattatcgcg
     5941 ggggtattta cgcctacaga agcatcggca attgcggtaa tttatagttt agccctggcg
     6001 atgatttacc gggaaatcac tcttaagaag ctcaatgata ttctgttaga ttcggtagta
     6061 accagttcaa ttgttctgtt actggtaggc tgctcgatgg ggatgtcatg ggccatgacg
     6121 aatgctgatg ttcctgagtt gatcaacgaa ctgattaccc gcgtttcgga taacaaatgg
     6181 gtcattctgt ttattatcaa tatcattctg ttgatcgttg gtacctttat ggatatcacg
     6241 ccggcgatcc tgatatttac acctattttc ctgccgatcg cccagcatct ggggattgat
     6301 ccgattcact tcggcatcat tatggttttc aacttaacca ttggcctttg cacaccacct
     6361 gtcggcacca ttctgtttgt gggatgcagc attggtaagg tcagtatcga cagggcaata
     6421 aaaccattac tgccgatgtt tctggcattg tttgtagtaa tggcaattat ttgttatttc
     6481 ccgcagctta gtctgatgct gccaggatta ttttcgacct gatacacaat gaagactgca
     6541 tactgagtaa ttttcaatgc cccaccactc tgtggggcat tgaattggtc cattacggta
     6601 ccggattgac caacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
     6661 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
     6721 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcgccat
     6781 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
     6841 gcaccgtcca gcgttcccac gttccttgct gcgcagtaac atcaatacgg ttgacgtccc
     6901 acaactgccc attaatcccc gggtcatcac ccagactgat atcacgactt cgaatcggcg
     6961 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
     7021 gaaggccggt tgggcgcagc gttagcacca gcgtagaaac cagaatgctg gatggctcaa
     7081 agaagccacg aatacgatca acaatgctcg ccgcttcgcc acaggtaatc gacacttcat
     7141 caccgttgct catatccacc agaatctcac gacgctcgcc cggtgccagc gaaagttgct
     7201 tcaccgatac aggagcgggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
     7261 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcaacaga cgcaagcgca
     7321 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagt agcgtatcgc
     7381 caacaaagcc gccgcttcca ggttcgttat attctggcgt accaaagtta tccagccgtt
     7441 tatcctgaat aatgacagga aaatcatcca caccataatg gttggggata ggcagcgatt
     7501 tactgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
     7561 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga atgggcagta
     7621 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggccag
     7681 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
     7741 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
     7801 cccaaaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
     7861 acagcggttg cccacggcga gattcaagca gcggcggaac gggtagcggt tgctgttgcc
     7921 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
     7981 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
     8041 tgcaggaaag tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc
     8101 gcgttctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
     8161 ggcagccaac tcgcgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
     8221 ttcgacaatc accagaccgt tgtgtagccg attaagatta atcttattcg aagttgtaga
     8281 gacgcacacg ggtataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ccccggtctt
     8341 gaacggcagc aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat
     8401 acggcgtttt ttgaagtgat tcactacttc cgcaatagtg ccgtgagctt tagtgcgatt
     8461 gtttctgtcg atcaataagt tgccggttaa ccaatacaac tgcccgaaga aggggatcca
     8521 cagcaaactc tttttaccta ccgtcactgt cggcggctgc acaatgttcg atgccgtcac
     8581 catgtcataa ttgttctggt ggttagcaat atagatagca ttgccgtagt tttcagcgtc
     8641 tgcaggtttg cggcactcca ctttcaggcc gaaaagcggc gcaagacgac caaacatatg
     8701 cccaaaggtg gccacatgtt tcggattacg cgggctgaaa aggcagtaaa tggagccgaa
     8761 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
     8821 accctctgaa ggtctgagag actggcattg tacgtcacct gaaaatcagg ttagggcatt
     8881 gttaaaagcc gctctctgga aaagcgtatt gttaatcgca acgcatgaat aaacaacggt
     8941 ttcaagggaa tttttatcgt ttccggggat tgaatacgct gccggatgac gacttaacgt
     9001 ttcatccggc gttccttgca agcgggagga aacagcgccc tccccggcat attactcttc
     9061 gctatcaccg ctgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa
     9121 accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg actttctgta gctcttccgg
     9181 acgcagttta attttgcgtt tcccaacatg aatggtcagc gtgctttgcg gcggcagaac
     9241 gtacagttgc gccaggccat cctcgccacg cgcggcttct gccgatggaa tgttgataat
     9301 cttgttgcct ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg
     9361 gcctgcctga gtgattgcca gcagcatatc ggaagcatct tcaatcacca ccggcggcat
     9421 aacatgggca ttttccggta aggtgatcaa agccttacct gcacggttac gcgccaccag
     9481 atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc
     9541 gtcgctttcc atcagcatat ggtcaacggt cgctccaggc ggcaacgtta atttgccggt
     9601 gagcggctcg ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg
     9661 accggtggaa tcaacaaaca ctaccggttg gttgctctta cctttcaccg ccgctttgaa
     9721 gctatcaccc gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg
     9781 tacccagccc atctgcgaca gcacaatggt gacaggttca gacggcagca tgtcgtgctc
     9841 gctcatcgct ttcgcttctt cgcgttcatg caacggcgaa cgacgttcgt caccgtagcc
     9901 ttgcgcgtct gcctgcagtt ctttcttcag caggttattc attttacgct cggaagccaa
     9961 aatgccctgc aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat
    10021 ctcttccagt ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc
    10081 cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca tcttcattac gaatgatctc
    10141 aatcacttcg tcgatattga gaaacgccac cagcaaacct tcgaggatat gcaggcgctt
    10201 gagaactttc tccagacgat agttcagtcg gcggcgcacg gtatcgcggc ggaacaccag
    10261 ccattcggag aggatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat
    10321 attgaggtta atgcgatagc tcttttccag atcggtggta gcaaagaggt ggttcatcac
    10381 ctgatccata tccacgcggt tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg
    10441 gtcagattca tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc
    10501 aatttgctcc agtacgcgcg cacctgaaac ctgatgcggc aatgcgctga taaccaccgc
    10561 gccatcttcc ttcttccaaa ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat
    10621 tttacgaatc tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg
    10681 cacgatatcc agcagctgat cgagcgtggt tttcggctgg tcgattaagg caattaccgc
    10741 ctgagccact tcacgcaggt tatgcggtgg aatatcggtc gccatgccga cggcaatacc
    10801 ggtggtgccg ttaagcaaaa tgtttggcag acgggcaggc agcattttcg gctcctgcaa
    10861 agtgccgtcg aagtttggca cccagtcagc cgtcccctgc cccagctcgc tcaatagcag
    10921 ctcggaatat ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc
    10981 gtccggcgcc ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg
    11041 cgccatcagg accatcgcta cataacaggc aatatcgccg tgcggatggt atttacccag
    11101 tacgtcaccg acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagctc
    11161 agacatcgca tacacaatgc ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa
    11221 cgcacggtcc atgatcacgt acatggagta gtttaagtag gcgttttccg taaattcatg
    11281 tagcgcaagg cgctctgcca tatcgctcat taattctgat tcctcaactt attcgccagc
    11341 cttcaaaggg gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga
    11401 tcagcgtgaa tagcgccgcc ggacgtgctc cggcggcgat aaaggcatca atgtgccttg
    11461 atatacaact ctttgctgta gacataatcc tggggattat gcagcggaaa accgccaacg
    11521 tagggtttaa gcagtttgat taacggctga tagtagatgg gaatcagcgg tgcctgctgg
    11581 ttgatgatca cttctgcctg ctgatacagc gcattacgct ttgttgcatc agccgtttgt
    11641 gccgcctggt ttagtaaggc gtcatactgc gcgtttttcc agtggccgac gttttcttca
    11701 ctatcgcttt tgagcgtgtt caggaagctg gaggcatcat tgtacgtcgc atcccacgac
    11761 tgccgagaca gcataaaatc accggctcgt cgggcatcaa gataggtttt ccactccatt
    11821 gtgcgcagcg tcacctgtgc acccagccat tttttccatt cggaagacaa cgctatcgcg
    11881 gtcttttcat gcagatcgta cttgttgtag aacagctcaa agcgtagcgg atgagaggcg
    11941 tcgtatcccg cctgtttcag caagactttt gccatcgcga cgcgctcact cattggcttt
    12001 tgcagttcat cgaacgtcgt cgcgctaaag ccttttacct ctggcggcgt cagcgtggtt
    12061 gcgggcgttc tcaaccccag taccttttgc gcaataagct gtcgatcaac cgtaagatat
    12121 agcgcccgac gcacccgcac atcgttaaat ggcggtttct caaggttaaa gttgtaatat
    12181 tcgctgttca gacgcgggat aattcgtagc tcgccaggca gtgatttttc aatggcggga
    12241 atttgctgcg ccggaaccca ggtgagatcg acctctcccg cgcgatagcg gttatagccg
    12301 gtgaccgaat tatccagcgc cagatactca acctgttgca atactgtatg ttgcgcatcg
    12361 cggtactttg gatttttgcg tgcagtaatc ttttcgttaa ctacccattt atcaagcaca
    12421 aaggcaccgt tgtaaaccat gttctctggc ttactccagc tatcgccatg tttagcgatg
    12481 acatgatgag gaaccgggaa cagcgtcggc caggcgagca tcgtcgtgaa ccaaggaacc
    12541 ggctgctcaa gcgtaacttc aagagtacga tcatccatcg ctttgacacc cagcgatgta
    12601 acatctgctt tacccgcaac aatagccgcg gcattgttaa tgtgcgcctg tgccagatag
    12661 ccagcaaaag ggcttgccgt tttcgggtca accgcgcgct gccagccgag gacaaaatcc
    12721 tctgccgtca gaggctgacc gtctgaccac tgcaaaccgc tacgcagatg gaaaatatag
    12781 cgcttgccgc cgtcaagtat ctcccagcgt tcagcctgag cgggctgcac ctggccttca
    12841 ccgtccatcc ataccagacc ttcaaacaga tccagcacaa tctgcgcggc ggtattctcc
    12901 tctacctttt gcgggtcgag cgtgcctggg tcgctatgat tgttgtaacg aaagacttgt
    12961 tgcggggcga gcggtgtgtt ggcgggaacg tcagcagcgt agagaggagc cgcacttacc
    13021 aggctgacca gccataataa atttcgcgta tacatggtat tccttaccgg agaaattata
    13081 agcccgacaa caatacgcct gtggcattgt ttgctcaaac ttatcgcgag tgatttggct
    13141 cacactccgg taaagattcc ccatcacgct aacggtacat aaacatccat ttccagtcgc
    13201 tgctctgtca tcccttcggc aagattggtg tagtgaaaca gaatcggtgc tttacgcatt
    13261 ttctcgccgc ttgcaggcag ccagtggcga atgatgcccc atatcgtgtg tgaaatatcg
    13321 tccagctcgc caacatggcg ggccacagca taacgtccac cggtaagttc accattactt
    13381 acgccataac gattatcggg gatcggttcg ctaacgctgc cgcagatatc aaagcgaaac
    13441 gcatctggtg cggtggtggc agggtcgtcc caggcgacgc caaaagtctg gctttggtta
    13501 acaggagaaa gccccgtctc tttacgccac atgataaatt tcgccacgct gtagttgagc
    13561 aactcagggc tacagcggtg cggcaacatc gccacaggga ttgatgggaa atcgataata
    13621 tttacatcca gtgtcaggtt tgtcattttg cctccctgcg aaattattcc cgggaacgcg
    13681 tcccgggagc ggtaacaatt acggatttac tttgcggatc tgtttgacgt caatttcaac
    13741 agagttccag tctttatcga cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt
    13801 cacgccgttc cagcgtttat ggtcgatatc aacattgata gtaccgctgg catctttgaa
    13861 cacgtagaga tcgtcagaga tgcgttcaac gatattgccg cgtagggtta cccaggtgtc
    13921 gtcacgcagg gattttgcgc tttctacagt cgttacgctg ccgttcggcc cctggaatcc
    13981 tccggcctga ctttgcgttg ccgatgggcc agaaaaacct ccctgctctg ctgccataac
    14041 cggtgcactg cacagggcca ttactgcgat tactgctgcg aattttttca tgtttattac
    14101 tccctttaat gtctgtttcc gagcatttaa caagatagtc cttaacaact tcttaaggga
    14161 aaaaaataaa atttaatgct gtacagagcg cgttacaaca cggtttactg gcagcaaata
    14221 cggttatcgc agggatgaaa aaatgcgaat tttactgata gaagatgaca tgctgattgg
    14281 cgacggcatc aaaacgggcc ttagtaaaat gggttttagc gtcgactggt ttacacaagg
    14341 tcgtcaggga aaagaggcgc tatatagcgc accttatgat gcggtgatcc tggatttaac
    14401 cttaccaggc atggatggtc gcgatatttt gcgcgaatgg cgagaaaaag gtcagcgtga
    14461 gcctgtattg atcctgactg cgcgcgatgc gctggcggaa cgtgtagaag ggctgcgtct
    14521 gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga
    14581 agctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg gcaacgtcat
    14641 gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa cccttaacgc tgaaaccaaa
    14701 agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc cgcgcaaact
    14761 gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg ttgaagtgca
    14821 tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc atggtattgg
    14881 ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgagt caggctgacg
    14941 ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa
    15001 caaacaacgg ataacgtcga tgagttgttc gacacccaac tgatgctgtt tgccaaacgg
    15061 ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca gacgccaaat
    15121 aaattaaaac acggtcatgt tgatgacgat gcgctgactt ttgccatctt tacccacgac
    15181 ggcagaatgg tccttaacga tggcgataac ggagaagata ttccctatag ctatcagcgg
    15241 gaaggttttg ctgacgggca actggtcggt gaagacgatc cctggcgttt tgtctggatg
    15301 acctcacctg atggcaaata tcgcatcgtt gtgggtcagg aatgggaata ccgtgaagac
    15361 atggcgctgg cgattgttgc cggacaattg atcccgtggc tggtcgcact gccgattatg
    15421 ttaatcatca tgatggtgct actgggtcgt gaactcgcgc cgctgaacaa actggcgctg
    15481 gcactacgta tgcgtgaccc agactcggaa aaaccactaa acgcgacggg cgtacccagc
    15541 gaagtgcgcc cactggttga gtcgctaaat caactgttcg cccgcacaca tgcgatgatg
    15601 gttcgtgaac gacgctttac ctccgacgca gctcacgaac ttcgtagccc gttaacggcg
    15661 ctgaaagtgc aaaccgaagt tgctcagctc tctgacgatg atccgcaggc gcggaaaaaa
    15721 gcgctgctcc aattacattc cgggatcgat cgcgctactc gtctggttga tcaactgctc
    15781 acgctatcgc ggctggactc actggataac cttcaggacg tcgcggagat cccgcttgaa
    15841 gatctcctgc aatcgtcggt gatggatatt taccacacgg cgcagcaggc gaacattgac
    15901 gtgcgactga cactcaatgc caacggcatc aaacgcaccg gacaaccgct attgctaagt
    15961 ttgttggtgc gaaatttgct ggataacgcc gtgcgttaca gtccacaagg tagcgtggta
    16021 gacgtcacgc tgaatgccga taatttcatc gtgagggata acggccctgg tgtgacacca
    16081 gaggcactgg cgcgaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc
    16141 agtggacttg ggctatcgat tgtccagcga atcgccaaat tgcatggcat gaatgttgat
    16201 tttgggaatg cgaaacaagg tggatttgaa gcgaaggtaa gctggtaact tctctgtcta
    16261 tgagagccgt taaaacgact ctcatagact ttactaatag caaaatataa accgtcccca
    16321 aaaaagccac caaccacaac ccagacaaat aacgagcgag aatacatata gccaaaactc
    16381 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggtg tcagaagaaa
    16441 aaatgctgca atcattgctt gtaatatacg atcttcgcct gaaacagaag gtatactgca
    16501 aatattgtct cccgactcaa gc
//
LOCUS       DAPQZD010000107        16261 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPQZD010000107 DAPQZD010000000
VERSION     DAPQZD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.107"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            67..1122
                     /gene="phoE"
                     /locus_tag="RO454_003287"
     CDS             67..1122
                     /gene="phoE"
                     /locus_tag="RO454_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDY0935077.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1161..1562)
                     /gene="crl"
                     /locus_tag="RO454_003288"
     CDS             complement(1161..1562)
                     /gene="crl"
                     /locus_tag="RO454_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDY0935078.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="RO454_003289"
     CDS             complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="RO454_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDY0935079.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="RO454_003290"
     CDS             complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="RO454_003290"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDY0935080.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3675..5132
                     /gene="pepD"
                     /locus_tag="RO454_003291"
     CDS             3675..5132
                     /gene="pepD"
                     /locus_tag="RO454_003291"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDY0935081.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5188..5802)
                     /locus_tag="RO454_003292"
     CDS             complement(5188..5802)
                     /locus_tag="RO454_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDY0935082.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5799..6938)
                     /locus_tag="RO454_003293"
     CDS             complement(5799..6938)
                     /locus_tag="RO454_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDY0935083.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7128..7580)
                     /locus_tag="RO454_003294"
     CDS             complement(7128..7580)
                     /locus_tag="RO454_003294"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0935084.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(7577..8632)
                     /gene="dinB"
                     /locus_tag="RO454_003295"
     CDS             complement(7577..8632)
                     /gene="dinB"
                     /locus_tag="RO454_003295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDY0935085.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8703..9626)
                     /gene="lafU"
                     /locus_tag="RO454_003296"
     CDS             complement(8703..9626)
                     /gene="lafU"
                     /locus_tag="RO454_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDY0935086.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9629..10492)
                     /gene="motA"
                     /locus_tag="RO454_003297"
     CDS             complement(9629..10492)
                     /gene="motA"
                     /locus_tag="RO454_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDY0935087.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10505..11221)
                     /locus_tag="RO454_003298"
     CDS             complement(10505..11221)
                     /locus_tag="RO454_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDY0935088.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11241..11708)
                     /locus_tag="RO454_003299"
     CDS             complement(11241..11708)
                     /locus_tag="RO454_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDY0935089.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(11716..12777)
                     /locus_tag="RO454_003300"
     CDS             complement(11716..12777)
                     /locus_tag="RO454_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDY0935090.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12774..13085)
                     /locus_tag="RO454_003301"
     CDS             complement(12774..13085)
                     /locus_tag="RO454_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDY0935091.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13090..13482)
                     /gene="fliS"
                     /locus_tag="RO454_003302"
     CDS             complement(13090..13482)
                     /gene="fliS"
                     /locus_tag="RO454_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDY0935092.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13505..14821)
                     /gene="fliD"
                     /locus_tag="RO454_003303"
     CDS             complement(13505..14821)
                     /gene="fliD"
                     /locus_tag="RO454_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDY0935093.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15231..16145)
                     /gene="lafA"
                     /locus_tag="RO454_003304"
     CDS             complement(15231..16145)
                     /gene="lafA"
                     /locus_tag="RO454_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HDY0935094.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         3757 a         4373 c         4076 g         4055 t
ORIGIN      
        1 actttcctgt tttcaacggg actctcccgc tgaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgtatcc
      121 gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagccatgc attatatgag tgataatgac agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg tcgttgggaa
      301 gctgagtttg ccggtaataa agcggagagt gatactgcac agcaaaaaac gcgtctcgct
      361 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ctcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggt
      661 ggcagcgatt tcgccatcag tggagcctat accaactctg atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat
      781 gccaataata tttatctggc aactttctat tctgaaacac gtaaaatgac gccaatctct
      841 ggcggctttg ccaataagac gcagaacttt gaagcggtcg ctcaatacca gtttgacttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga gggcatcggt
      961 gatgaagatc tggtcaatta tgtcgatgtt ggtgctacgt attatttcaa caaaaatatg
     1021 tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     1081 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatggatatt gccggatgtg
     1141 atgcatccgg cacatttcat tcacgccgtt aacttcaccg gttcatcacg aaaatcatct
     1201 gccggttcca gcttcagatt cagcgtcgcc agcagctcac gcagcttctc gtgaaattca
     1261 cgcagggtgt gctccagtcg ttcaaccact tcggcatctt ttatctgaac actcgtccag
     1321 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     1381 tccagttcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     1441 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     1501 gggcctagtg cggtaaattt tttgactaat ctgctcttcg ggtgtccact cggtaacgtc
     1561 attgcaatct cctgttgtga tgcaactgtt ttaccaaatt ggtaaaattt agcaaatttt
     1621 taacacaaac gtttttcgat ccagtctgtg atttcctgaa ggcctttgtc aaaattccga
     1681 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgacga cgtgattaag
     1741 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt
     1801 gggcagcgac gtcccagcaa cccttgcact tttaatgaat agcgattcag ctccacgcgc
     1861 aacgcttcgt cagaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     1921 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     1981 caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgtacggc aacgttagcg
     2041 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcaac
     2101 gcctttaaaa cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgaagaa
     2161 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcagcgc aaaataacgt
     2221 tcatacaggc tgtaatagtc tgtctgcatc gcatccagac caccacacat taatactgtc
     2281 gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     2341 acggtaaact ccatctgccg catcgtgccc ggcagacgct gagcggcctc ttcataggcg
     2401 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca
     2461 gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac cgctgagttg cggatcttct
     2521 tccgcacagg ctttttgttg ccacaccatc gcctgggtag cccactcata aatccagttg
     2581 ccgccgcggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc
     2641 atcacaatac gcgcctggac ttcgaggatt tcacgggggt caatgccgcg ccagatccac
     2701 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc
     2761 acaggcagtt gtgcgccgtg actaaagcgg cggactagcg tcgaggtttc aggatgttta
     2821 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatagatgc agcctccaga
     2881 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     2941 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     3001 cggctgttcg atccaggtat tttgcgggat atcaacaaca taatcatcaa ccagcggacg
     3061 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     3121 aaccgcagta ccaccggtat ctaccaggtc atcaataacg atgaagcctt cgccatcgcc
     3181 ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     3241 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     3301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     3361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     3421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     3481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     3541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     3601 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     3661 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     3721 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     3781 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc
     3841 tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc ttgcaggccc
     3901 acctcgatat ggtgccgcaa aaaaataacg acaccgtgca tgacttcact aaagatccta
     3961 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcggata
     4021 acggcattgg tatggcctcc gcactggcgg ttctggctga cgaaaacgtg gttcacggcc
     4081 cgctggaagt gctgctgacc atgactgaag aagccggtat ggacggtgcg ttcggcttac
     4141 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     4201 tctacatggg atgtgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag
     4261 cggttccagc tggttttgaa accttcaatt taaccttaaa aggtctgaaa ggcggtcact
     4321 ccggcggtga aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctgg
     4381 cgggtcatgc agaagaactg gacctgcgtc ttattgattt caacggcggc acactgcgta
     4441 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgccc
     4501 tgaaatctct ggtggatacc tatcaggaga tcctgaaaaa cgaactggca gaaaaagaga
     4561 agaatctggc attgctgctg gactccgtag cgaacgacaa agctgcatta acggcgacat
     4621 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggagttatc cgtaattccg
     4681 atgtagccaa aggcgtggtt gaaacctccc tgaacgtcgg tgtggtgacc atgaccgaca
     4741 ataacgtaga aattcactgc ctgatccgtt ctctgatcga cagcggtaaa gactacgtag
     4801 tgagcatgct ggattcgctg ggtaaactgg caggtgcgaa aaccgaagca aaaggcgcat
     4861 atcctggctg gcagccggac gctaactctc cggtaatgca tctggtacgt gaaacctatc
     4921 agcgtctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     4981 tgttcaaaaa accgtatccg gaaatggaca tggtgtctat cgggccaacc atcaccggtc
     5041 cacactcccc ggatgagcaa gttcatatcg aaagcgttgg ccattactgg acactgctga
     5101 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     5161 aacgccttcc ggcagtttca tccttcatta tccttcgata aaagccatcc ctgtgaacgt
     5221 tcgtcgcggg ttgccacgtt caatctggtg atggaacatt cgccgctgcg atttcagcgc
     5281 cgcgctattt tcctgttgct gttgttccag cttccaggca atcagtaatc gcgccagccg
     5341 tttgttagca tgctgactgc gctctgactg aaccttcacg ctaataccgg atgccagatg
     5401 cgtggcgcgt accgccgagt cggttttatt gacatgttga ccgcccggcc ccgacgaacg
     5461 cagcgtctca taacggattg catccgattg ttcctgctca tcagcggtaa aacgcccaat
     5521 gcccagaaac cagtttttgc gcccatgatg aggccgatac ggactcggac aaatccactg
     5581 aatagtgccg caccagcttt cgcttagagc ccatgcgtta tcgccatcca gagaaatcag
     5641 cgccgaacgc agtgtgtcag agtagcgacc cgtttctgtt tccagcaccg ttaccgcgac
     5701 gtcctgtcgg gtagcttctt taatcagcct gtccagtgct tttttcactg cgagacaaca
     5761 ttcttccggc ccctgagcag aggagagttg tagcaagatc atcctttttt ccctccactg
     5821 tttttgagcg tcagcaccgg gcgcagtcgc gcgacaggaa taattaaccc agccagcacc
     5881 agacattgca ccacgctttc agccgattta taggcttgtg gcgcttcttc aaagatgagt
     5941 tgtttatcgc gacaaattac ccgactgcca agttcagtcc gcgagagttg cgtcgccgtg
     6001 tatttcgctg ccagacgccc tttacactcg gtgcgccccc atttacgccc cgccccatgc
     6061 gccagcgagt ggagcgtttt ttcgttcgca acaggtttaa ccagccagga gtaatcgccg
     6121 cgtgaaccgg gaataatcac cagaccgttg tcatccggcg tggcgccttt acgatgcaac
     6181 cagccctgtt gatcgccgat ttggcacgcg ctaacaaagt tatgcgccac atccagaacc
     6241 ggacttccgg tggccttaac ttgttgcatt atgcgcaaag cgatcagctg gcgattaata
     6301 cgcgcaaacg ccagcgcatc gtcatgttcc gcaatatacc gtagcgcgtc gtcactgcct
     6361 tcaggcaaac catgatgcga aaacgaggca atatgccgct gtaaaataga ctgccccaat
     6421 cccctcgagc cgctatgaac cagtagttgc agatgctgcg catccagacc agcccgtgca
     6481 aacagttccg cgtcgataat ttgatcaatc tgttgcagtt ctgcgaagtg gttaccgcca
     6541 ccgatggagc caagcgagtt gtgccacgga tgctgtgcaa atgctgacgg caggttttcc
     6601 tccagccagc tttcttcagc aacgtcatcc agatctgata atcgcttttc aaacttatcg
     6661 gcgttgtatt tgcgagcgag aatatctgtt tgccatagcg ccatgccgca gccgatatca
     6721 ttgccgacca gtgccgggta aaaacgacca acagagaaga acgctgcgcc aatcgggtag
     6781 ccgcgtccgg gatgtaaatc tggcatcccc acaacgcgct gcatgttggg taaatttgcc
     6841 gtggtgtgta attgttggat cgctaaactc tcgatccaca ggtcatcaga tgcgatggta
     6901 aataccgcat cggataaggg acgaatatat ttgcccatgt accattccat ttatgaataa
     6961 aaatcaggaa atggcagacg aatccagggc aagaaaaaag aattaatcct ggaaaggtct
     7021 gcaaagagtc attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag
     7081 aaaacgcgca catactagac gggcgttatt ttcattgcaa gctggattta atgttgcggt
     7141 ttatatcgca tataaaaatt agtaaaccac gttccccggc attcaacgcg ctgctgctta
     7201 actgtctgaa aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc gtccaccgta
     7261 agttcggatt cagacttaat caatggtttt aacaaagcgc tggcaactcc gcgtcgggtg
     7321 tattccggtt caacaaataa catatcgata taatgtccaa tgcgggtaat aaaaccaacc
     7381 ggttgtgcat taataactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga
     7441 gattcgtcaa tctgcgccca ggcggcaatt tgctgtggag aatagtgctg actcgccgtc
     7501 atcgtaacag ctcgaaggaa aatagcgcat agttgctgaa aatcaccagg ctgatagttt
     7561 cttatttgta tgttattcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
     7621 acgtcacatg cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt
     7681 tacgcgcggt ggcgattaga tcagatttat tcagccgcgg ccagacgtgt tcctgggtgg
     7741 tttgctgaaa atcgtcgaac tttaatttca ccccctggcg ggcaatcagc aaatcaggtt
     7801 ttacctttgc caaacgacgc tccagttcag ggtaaagccg ctcgatgatc gcttcacatt
     7861 cagaccagtg atgaatatct tccgccatcg tacgttctac accgacggat tttcgcaacc
     7921 tttcgctgtt aacgtcgcgt tcgtcaatgc cctgactacg ctcccacaaa atgcggccaa
     7981 atttgccaaa gcgtttaagc agcatcacca gatcacactt ttgcacatca ccgcaggtcc
     8041 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccggga atttttgcca
     8101 gcggtaaggt ttgtaaaaat gtcggaactt ctgccggcgt aatcacaaac tggccgttgg
     8161 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagacg
     8221 ccgtcagttg cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag
     8281 aaccgtggca atggacgcta tcggtgacat cgagataggc ttcatccagt gataacggtt
     8341 caatgcgcga ggtatagcgc gagaaaattt cgcggatatg atttgaggct tctttgtagg
     8401 cgtcaaagcg ccctggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg
     8461 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
     8521 gtcgttcgcg gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca
     8581 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc
     8641 tctcaatact ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc
     8701 atttaacgcg ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
     8761 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
     8821 agcaccataa tctcaatgcg ccggttgccc gcgctttgcg gatttttggc atccagcagc
     8881 atctggtccg ccattgcact tacctgcatc actttatctt ccggcattcc ggcctcttcc
     8941 agcacccgac gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg
     9001 tttttgtagg ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg
     9061 aacactggcg caagctccac cagcaaagtt ttaaagaacg gcataatctt cgcgctgccg
     9121 cgttcgaaca tattgcggtt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca
     9181 atctccatct ccagattcgc ttccatatgc gcatcacggg cgatggtatt gatactggtt
     9241 gccagctcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcgttgacg
     9301 tcgcgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
     9361 gacgggctga ttttatttaa cggcgacaac ccaccaccgt taaaaatcga ctgcccgtgc
     9421 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat ccacagcgtc
     9481 ataaatagcg ccatcattgc caaagtaaag tcggcgaacg ccaccttcca cgccccgccg
     9541 tgatgcccgg catgattctt tttgatctgc cgccggacga tggtggtttt tgccccacgt
     9601 tcgcggtggt tgcccgtctt tctcatgctt attcttgctc cagcatcgca ttcacccacg
     9661 cgtcgaggtt ggcgaaggtc ggtttggacg ccagatgcag cagcttacgc ccggcgtcca
     9721 ccgccagcag cgtcggtttg ccgcctgcct gcgccaccag cacagtgcgc acgcattcca
     9781 gcaaagaatg ttccgcccgc gcctgctgtt ccatggcgtt ggctagcggg tccatcaggc
     9841 agtagcagat aaacacgcct aaaaacgtcc ccaccagagc cgccgccact ttcaggccaa
     9901 tcagagcgat agatccatca atcgactgca tggtgatgat gatgcccagc accgccgcgc
     9961 aaataccaaa tcccggcatc gcttcggcgg tacgttgcag cgagcgcgac ggcgttaaca
    10021 gggactcttc cgcggtgtcc agttcctgat cgagaatgcc ttccagctca tgggcgtcga
    10081 ttttacccat cgccatcagg cgaaagttat cagcaataaa ggtcaccagc cgcttctggg
    10141 ttaacaccag cgggtatttc tggaaaatgg tgctctcttc cggctgctca atatgctgat
    10201 ccaacatccg cagaccgccg ttttgcacca tttcgagcaa ctcatacagg cacattaata
    10261 gctgacgctg aaactccggc ccgagctgtt ttttactgat cacgccttta atttgatggg
    10321 caatctcttt cagcacatgt ttcgggttac caataatcat cgccccgaaa ccggcaccca
    10381 aaataatgat catttccgcc ggttgccaga tagccactaa ttgcccgccc gcttcaaaat
    10441 aaccgccaaa tacgcagccg agcaccacca gtaaacctaa tattttttgc atgatttctt
    10501 cactttaatt cggatagaca aaatcacgaa ttttctgcgc gatctgttta ttcagttgac
    10561 aaattcgggc ctcggttagc cctaatacca gcgcaatctc tttgagattc atctcatgct
    10621 ggtaatacat ggagaggatc agttgctctt tttcgcttaa ttgtgaaagc gcgtgttgca
    10681 acatttgctg agtgacaaac tgatcttcca gttcccggcc agcaagcgga atgcctggcc
    10741 cttcgccgtt cagcagttcg tccaggctgg cgagcgtttc cgccccttcc agttgctgat
    10801 attcgagata atcttcatgc gagatccctt cctcagccag ctcctgccac tccggttcgt
    10861 gccccagttt tttacgcgtt tcgcgaatca ggtctttcat ctgatgatat ttctgtcgca
    10921 gctggcgcgg tcgccagtcg agagcgcgca gctcatccag gatcgcaccg cgaatacggt
    10981 gcacggcata gcccgcaaaa ccttcgtccg gcagcccata gcgacgaatg gcgttcaaca
    11041 gccccattaa cgccgtttgt tccatatcct gacggtcaat aatgcaatta cactgcgggg
    11101 caagttgccg caccacttta cgcaccaacg gtaaataggc ctgcaagtag tggctctcct
    11161 gctgcggagt taataccagc gtggctgcaa attcttctga ctcgaataca tcctgtagca
    11221 tgtttatatc cgcctggtga ttactggaag accacttttt tcaccagcaa atccttatac
    11281 ggtgcggtca ttttgcgacg ctgaagatct ttctttaacg cgtcagaaat cagtttgcgg
    11341 atggcagata ttttcatgcc gcgcaaatct tcaaatttca tttccgtcag gcattcaacc
    11401 gcaatacttt gatacagcgg ttcctgatgc tttatttttt ccgcctcttt gtcactggcg
    11461 accaccatca ctaattccgc cgacaaataa tgatccgcgc cgttgttgtc atgcaaagtg
    11521 acaatggttt ccggcagcgt cacaaatacg ctcttgctct catccagagc ttccaccgat
    11581 tccgtttgcg cggcggtagt cggttttcct tttccggcat aatgatgcca gacactccat
    11641 cctgctccag cgccgaccac caacgccaga acggcgctga ccacgcctgc caccacgatt
    11701 ttcttcatat tgcctttaca ctttgatcaa aacggattcg ttctgttgaa attgaggcgc
    11761 atcatcaagc tgtaaggcgg cactcacctc ttcctgctga tggcccgact gttgttgctg
    11821 ctgcgactgc ccttcggagg aaacctgtac gttcacctcc ataaaatttt gcgccgtcag
    11881 atgctggcgg agatcgtcgc taaactgctg caatgcgcgg cagacttcgc tttgattcgc
    11941 accgatatgg accgtcagtt tgccgttatc caactgtacg gcgatctcaa gcttgccaag
    12001 cgacggtgga tcgagacgaa tggtggagat ctgctgttgt ttgttgagct gaaagtggat
    12061 ctgatctttc aacagcgtcg tcagtttttc gcccagttcg tccatcgaaa gcgtggctgg
    12121 tttggcgaca atctcctgat gatcgctgac caactctggc gtcttaacct gcaacaccgc
    12181 acgcgcaata accggttcgc tgtccggcac tttttccgtt ttacgctcga cctgcgggcg
    12241 cgcggtcaca cgggttagtt caggtttcgg gcgagctggc ggtgttgaga cgcgcggctg
    12301 cgtcggcgca atggcatgta aatcttcact ggcgtaagtc gccagtctgg cctgctgttc
    12361 gggtttcgcc tgcggcaact gaccgatcag ttcctgcaac tgcggcggca acgacgtgag
    12421 ttcctgctgc tgcgtcgggc gttgcggcgc gttttgcacc accgctttcg tcaattgctg
    12481 gataaccggc gatgttgcga cgtttcgcgg ctgcggcgtg tcctgatgcg gcgcggcagg
    12541 ttgcggaagt aacagcgcca ttagcgcctg catcgccacc ggatcgttct cctgcgggcg
    12601 cgtcgcctgt tgctgactgg ttttagcgct atgaacgcgc tccgccaccg ccgcgaccac
    12661 cattttcggc agggtaaaca cgggcgcgtt ttcaccgctc accggcggta aaatatccgc
    12721 cttcaacgaa gcggtttccg caagcgttcc gagcgtggca agcaatgccg gattcatgac
    12781 ttctccgaaa gtaaattaac aattgagtag gccagtacgc cttcctgatg ccgctgatgc
    12841 tgttccatac gcgctttcaa cacttctgcg cgctgctcgc gccgtttaat cagggcatta
    12901 aacgcgccgt acagttgtgc cttcaccgca tccaactgcg ccgcgtcggt ttccgccgcc
    12961 gcgtaatcgt gaaactggcg catcaactgc tgatacagtg ccggaaggcg tcgccagcgt
    13021 tgcttgtcga gggcgtcatt catcgcttcg accagtgcaa aaagttgcct gcgttgtttt
    13081 tccatcgtct taacccagct tgcccgaaag cccttcccag ccttcgcgca ggttgcttaa
    13141 gatcagcatc acttcatcga ttttctctgc cgacagttcg ccgctggctt catacaagcg
    13201 atacacacag tggtcataga ggcgcgaaag gttgaccacc agctcgccgc cggtttcaaa
    13261 ttccagcgaa ctggtgagcg cattgagaat gtcgatgcat ttattgatgc tctgggcttt
    13321 cttctcgaaa cgccgccctt caatgtggct tctggcgcgt tccagctcgt ccatcaggcc
    13381 agaaaacagc accagcacca gttcaagagg cgatgccgcc gcggtacgcg ccgcgagatc
    13441 gatctctttg tattgcgaat aaccgtcctg tacttggtac atctttaagg atattccttc
    13501 tgaattacgc aaatgccgcc atactggctt tcatggtgta cacctcaacc aacgtattgg
    13561 tgtactcctc gagataacgg tcgtagttag cgttataggt atcggtaagc tgatcgcctt
    13621 cgtcctgaat tttgctcatc tgatcatcaa tgttctgctg gcgcagggtg atgatgccgt
    13681 tgctggagtc cgtgtaggtg ttaatcaggt cgtccatctg cgccaccatg ctgttgtcgc
    13741 caacgaaaat agaggtcagg ccgtcgggat cgctcttcat cgcgtcgttg aactggtcgg
    13801 agtcaatctg taagtggccg tgagaatcga gcgtgatgcc atagtcgaca atcgacacgc
    13861 cgttgtagct ggcatgggcg atgtcatcga gctggtttgc cagtgagctg agtccagcat
    13921 cccccgcaaa gaccccggcg ctactactgt catcaccgtg ggtggttagc gaatcaaccg
    13981 tatcaatcaa ggtgttatag gcatcgacaa aggtctgtac tttctcctgg ctggcgctgg
    14041 aatcttcact gatgttaaag gtggtgagat cgctgtcaga atcgctgact tcggtaaaag
    14101 tcatggtgac accggggatc acatcatcaa acgtgttgct gctgttggtg atagccggtc
    14161 cgctggcgct accgagatga ataatcgcgt cctgagcgga ggaaacatcc gtggcaacgg
    14221 gcgcactggt gctatcgttg ctggcatcat gccccgttac gctcaccgaa aacgcgctct
    14281 gcgccccggt gctatcggag gtgagcataa tcgtggtggt gccgtcggtt tttaccagtg
    14341 ccgccgacac gcccggattg tcatcggagt cgttaatcgc attcaccagc tcgctggcgt
    14401 cgataaaacc gtcaccgtca ccgttctgat ctgccgccga cagatcgata tccatagtgc
    14461 tgtcgcccat cgtcagttca aaagtaccgg tagcggaaaa ggcgtcgtcg cccatactga
    14521 acgtggtttg ctgcccctgc gccagttgct caacgaaaaa cgagtagctt cccgcctgcg
    14581 cctgggaatt ggcggaaacg gtcgccgagt cattattgct ggtggcggca aaagtcaccg
    14641 ggccgtcggt atcgctgttc agcgcgtcaa cggcgctctg gaaatcgctc aacgccgagc
    14701 tgagcgagtc caggccgctg ctttcagcat ccagctcact ctgcttctcc tgcaaattcg
    14761 tcgcctgagt agcgacatcc gcatacgcga tttcttgcgc aatggttctt gggttgatca
    14821 ttgatgactc cggataacaa tgtcaggtta ttggcaaaag gcgtgccaat attttttatg
    14881 tgaataatca atagattgaa aaacaggcgg aaatgacgtt tccggtctgg cggaaaaaag
    14941 cctgccgcag aaagcacaac accgcccaca ggcggtgttg ttcgtggccg gataaggcgt
    15001 ttacgccgca tccggctatc ggtgcacagt gcctgatgca acgctgacgc gtcttatcag
    15061 gcctacgagt ccgcgcctgt ttttatgcgt gtgcccattt gtaggccgga taaggcgttc
    15121 acgccgcatc cggcaatcgg tgcacaacgc ctgatgcgac gctgacgcgt cttatcaggc
    15181 ctacgagtcc gcacatattt ttatgcttgt gcccgtttgt cggcccgaac ttactgcagc
    15241 aggctggaaa ccatgctgga catgctgttg gactgtttca gcatggtgat accggtctgc
    15301 atcagcacct gttgtttggt catattggac gcttccgtcg cgtaatcggt atccatgatg
    15361 ttgccgatag ccacttcggt gttgtcctgc atgctggtca ggttgttggc ggtgtcattc
    15421 agacggttga tagatgcacc caacttggac tgaatctgag aaacgtcatc catcgcggta
    15481 gaaatgctgg tgatcatctg gttagccgag ccagacgcag tcagttcagt accgccagaa
    15541 acgcctgcgg tgccagtggt gtcagcctga tccgcactaa aggagttgct gatagcagaa
    15601 aggtcagtca ccagcgtgtt cagttggctt gaaatgttca cggtcatggt gtcggaagat
    15661 tccgcgccca cctggaaagt taccgcgctc tggaacagac cgtcagtacc ggtgttgctg
    15721 gtgccggaaa caccgaacag gttggtgccg ccgtaagtag tgttttgcag catgtcagac
    15781 atctgctggc ccagttcgtc gtattcgtcc tgcatcgcct gcaggtcgtc gtcgctgtag
    15841 gtgccgttcg ctgcctgcgt tgacaggtct ttcatacgcc ccagcacgtc agacatttcg
    15901 tcgaacatgc tgtcagcggt ttgcagcatc gcggtagcat cattgatgtt gctcagcgcc
    15961 acacccatac cgctggactg tgccgtcaga cggttagcaa tttgtttgcc tgcggcgtca
    16021 tcggcagaag agttaatccg gttacctgtc gccaggcgtt ccatagaagt agacagagaa
    16081 gagctgcttt tgctgattgc attaacggcg gccattgagg cgttattggt gttaatagat
    16141 aacatgatac tgctccttgg gattaatcgt gttcgtttcg ataccctgta aaaacgacac
    16201 ctcgggagca aacttaaaat cgccgttaaa attttttcgc cacgtattat ccgatgccac
    16261 t
//
LOCUS       DAPQZD010000108        16170 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPQZD010000108 DAPQZD010000000
VERSION     DAPQZD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.108"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(992..1252)
                     /locus_tag="RO454_003305"
     CDS             complement(992..1252)
                     /locus_tag="RO454_003305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935095.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            complement(1278..1841)
                     /locus_tag="RO454_003306"
     CDS             complement(1278..1841)
                     /locus_tag="RO454_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0935096.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(1889..3250)
                     /locus_tag="RO454_003307"
     CDS             complement(1889..3250)
                     /locus_tag="RO454_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDY0935097.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            complement(3302..3532)
                     /locus_tag="RO454_003308"
     CDS             complement(3302..3532)
                     /locus_tag="RO454_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935098.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(4567..4758)
                     /locus_tag="RO454_003309"
     CDS             complement(4567..4758)
                     /locus_tag="RO454_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935099.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(4755..5177)
                     /locus_tag="RO454_003310"
     CDS             complement(4755..5177)
                     /locus_tag="RO454_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDY0935100.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(5224..5526)
                     /locus_tag="RO454_003311"
     CDS             complement(5224..5526)
                     /locus_tag="RO454_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDY0935101.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(6066..6842)
                     /locus_tag="RO454_003312"
     CDS             complement(6066..6842)
                     /locus_tag="RO454_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935102.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(6893..7327)
                     /locus_tag="RO454_003313"
     CDS             complement(6893..7327)
                     /locus_tag="RO454_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDY0935103.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            complement(7341..7562)
                     /locus_tag="RO454_003314"
     CDS             complement(7341..7562)
                     /locus_tag="RO454_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935104.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(7563..8246)
                     /locus_tag="RO454_003315"
     CDS             complement(7563..8246)
                     /locus_tag="RO454_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDY0935105.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(8323..8627)
                     /locus_tag="RO454_003316"
                     /pseudo
     CDS             complement(8323..8627)
                     /locus_tag="RO454_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8631..9533)
                     /locus_tag="RO454_003317"
     CDS             complement(8631..9533)
                     /locus_tag="RO454_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDY0935106.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(10400..11371)
                     /locus_tag="RO454_003318"
     CDS             complement(10400..11371)
                     /locus_tag="RO454_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDY0935107.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(11371..12537)
                     /gene="sopA"
                     /locus_tag="RO454_003319"
     CDS             complement(11371..12537)
                     /gene="sopA"
                     /locus_tag="RO454_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDY0935108.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(13125..13880)
                     /gene="repE"
                     /locus_tag="RO454_003320"
     CDS             complement(13125..13880)
                     /gene="repE"
                     /locus_tag="RO454_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDY0935109.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(14654..15460)
                     /locus_tag="RO454_003321"
     CDS             complement(14654..15460)
                     /locus_tag="RO454_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDY0935110.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(15461..15766)
                     /gene="ccdB"
                     /locus_tag="RO454_003322"
     CDS             complement(15461..15766)
                     /gene="ccdB"
                     /locus_tag="RO454_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDY0935111.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(15768..15986)
                     /gene="ccdA"
                     /locus_tag="RO454_003323"
     CDS             complement(15768..15986)
                     /gene="ccdA"
                     /locus_tag="RO454_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDY0935112.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
BASE COUNT         3863 a         4510 c         4140 g         3657 t
ORIGIN      
        1 aaagactcca aataccagaa tcaaactatt caacaaatag tgttttttct caaacaagac
       61 atcgctagcg aaaaaatatg aacagaaatt tcactacaat aattgcaggt aatcagcaca
      121 attattattc atggtgaatt tttctagcag ttggttacca gttactgcaa ttaagctaac
      181 tgttagccgg tagagagtga atattctcct gttactccct gttccgcgaa accatacaag
      241 ctttcagcga tgatgtagta taagtaagcc cttttaaccc cgttagcgta gccgcgcagt
      301 atcggaaact tggtactgac aagcctgctg gcagtactta agggctgtac gactgaattt
      361 aagcgatttt ttcttgacag aacacggtag ctgttgagtt tcgggagccg cagttttgac
      421 gttttgcggg gacctgcctt ttgttccctc tggtattcac catcacctgt ttgagaaccc
      481 tcgccgcaag gggcatcact gccacggcct gacgcacctg tcctgggcag aacgcaaaat
      541 tttttaccgg gttttgggta aaaaattttg tgggctgttc atttaccctg gacagtcagt
      601 gcggcaggtt gtggctgtat cagcccccgg cgacaggggc ttaaacaggt gtatggcacc
      661 ggacacaaca acgggcaggc acccgccgca acgacgaaga gcgcgtgcgc gaaacggcgt
      721 tgcccctcac atcccggcgg ccttttgccg ggatgccagg agcgcaggcc acggcgcgac
      781 agaggcggcg cgaccgttcg gtcgctgcaa gcccggggtt ttcacgcatc ggaccgtagc
      841 gacagcccga aaccgcgcca gcgggttcgg cggagactgg caggtgcgcc agcagctgcc
      901 tgtcggagtc gggcctgacg accggatgcc gccaggcagc cgggagccga aggtgcgccc
      961 ggcagacagg acaacactgc agtagtcaga atcagagcag caggaaaaag aagaacagta
     1021 gcagtagcag tagcagtagc agtagcagta gcagtagcag tagcagtagc agtagcagta
     1081 gcagtagcag tagcagtagc aggaacaaca acatcagtaa cagacaaatg acaggcatga
     1141 agaaacagaa gcccccatca gaacgtccgc gtaatgaaag gggcggcgcg tgagcgcggc
     1201 ccctttcagc gccaccggcg cgcgtttttt cccggcgcgg gcagacaggc atttatcctc
     1261 ccgccggccc tgtgtcatca ggcacgcaga cgaataatca tcgtcgggac atcggtataa
     1321 gcaaacgtgc cgcgcggcag ctcctcgcgg acgtcagaaa acggtaacag cttctcctgc
     1381 tggcgtggtc cgttgagaca gacggcgacc agcacgccac ccggacgcaa cagggaaaag
     1441 gcccgcagga tatgccggat atcctgcccg tggctgaacg gcggattcat gataacccgg
     1501 ctgtaatact gcaccggcag ccattccatg aagtcgccac actgaaccct gacaccgtta
     1561 aaattttccc gcagataccg gaccagcccg ctgttgattt ccaccgcatc acacatggcc
     1621 cccggcgcgg tatcccgaat cgcccgcagt atggccccgg tgccggcaga gggttccaga
     1681 atgtgatcac ggttgctgat ctccgccagc atcaccaggc gatcacagac cggggcagga
     1741 gtcacaaaaa gctgagattc agcccggatt tcagcaaccg gcgcggcgtt atccagcctt
     1801 gcgcgcatca gccggatacg ttctcttaac tgactgtgca tatcattatc tccgtcatcc
     1861 cccggggatt ttcccggggg gatatacctt atttctgtgg gatttccgtg attttcatgt
     1921 cggagatata cacattcacc aggcggaaat tattgtccat tgtgcggcgg taccggtatg
     1981 ccccgtggtc ttctgcttct gccacactgc gcacggcctt acagtcccgg ggcagggcgg
     2041 cccactgtgc ctttgtcatt tcccggaaac cttcccccgg atagttgact accggcggac
     2101 gcttcgcggc ctgactggcg acggcagcct cttctgccga tggtgctttg tagtccgtga
     2161 tgcgatcggg cgtcactttc atcgtgccgc tgtacccgag aaaactgtaa tttggcgttg
     2221 tgactgaact caccgccccg ttgcttttgt tcacacggat gatggtcagc cactcgcccc
     2281 ggctgaaaac ctgtccgccc ggctcaaaat cctgtgtccg ggtaaccacg ccgccgcttt
     2341 cgtccagcat ggcacgctca tagatcaggc ggttctgata gtggttaacc cagcgttgtt
     2401 gatgctgaat ctgccgttca tgacagcgga tcgcaatttc gcgggcctgc tccgtggtga
     2461 tgatgtcatc atccagggcc gaccataacg atcgacttcc ttcatactgg cttttttctg
     2521 ccgggcgcgg atacgtatcc agcgggaaac aggcgctgat atggtcatga ctgctgatga
     2581 gttttgccat attcagatcc agcgattcag cccgccacat cgtcagatat ttctgcgact
     2641 gcgcgatggt cttttcagcc ttgcgcaaat cagcttcgat ttttttgata cgacgccagc
     2701 gaacgtccgg acgttcttta tacttcgcgt gaagcagtgc cgaccgcgcc cgctcttccc
     2761 agtattccgc acgttcaaag agcatcacgg ctcgtttcat gccgttttca atacgctgcg
     2821 catcacgacg ggcgcggcgt tcgctgtgat gccccacaag aatgggctga ccgggcggga
     2881 tcatcgcggc cagtctttcc acttcatcaa gtgcctgtgc ggattcactg gcccgctttc
     2941 cgctgtatcc ggtaaaccgc tccgcccgtg cttcctgacg ttcagcgagc gtgctgtctt
     3001 catcctcaat ctctccggca agtgagagga gcacgtcttc ccggcccggt gtccaggctg
     3061 gcgcgacaaa cagcgcctgt tttggtgccc agcggaaacc ggcatcatgc acttttttat
     3121 acgtctccgg gtcaagacgt gacacggcat acaggcgcag tttgttgtca tccggtgagt
     3181 aggtcgcgcg gtacatctgc acagggctgg cggcttccgg ggcggtcatg gtgattgctg
     3241 ctaatgtcat aaaatgttct ccttccgtct gttgccgccc ctcgcggggc ggctcacctc
     3301 atcagttaaa agcgcgtttc agttcgagtg tgcggagcac ctgcccgaag gccgtgtttt
     3361 tcatggcggg gatgtgtaca ccacgccccc ggtgaaaact ccgggcaatg tgtcgcccga
     3421 aggctttcag cacggggtca atttccggcg cggggatgtc ttcgcgtctg aaacggggat
     3481 gacggcgggg ctttacagcc gctgcggaag gtgaaacaat acttactgtc ataatttagc
     3541 ctcttatgac gttaattaat gaaaccgtaa agactgttcg tgcagtctct gcggtgtgga
     3601 ttaagcaggt ccctgacctg cttttttcat atctgcgtct gacgttctcc ggtgttttcc
     3661 tccttctgtt cctgcgttct cattacctgc cagccggatg actgacagaa cacataaatg
     3721 cgtattgccg gagcctgaaa taaatcctga tttttaaaga acatcacccg ttgagtttcg
     3781 ggagccgcgg ttttgacgtt tcgcgggaac ctgctgctgt ataccctgtg gtatgaactt
     3841 cacccgtttg agaaccttcg ccgcaagggg catcactgcc acggcctgac gcacctgtcc
     3901 tgggcagaac gcaaaatttt ttaccgggtt ttgggtaaaa aattttgtgg gctgttcatt
     3961 taccctggac agtcagtgcg gcaggttgtg gctgtatcag ccccaggcga cagggactca
     4021 tacgggtgtg tggcaccggc aggcaatgag cagggacccg ccgcaacgac ggagagcgcg
     4081 tgcgcgaaac ggcgttgccc cttacatccc ggcggccttt tgccgggatg ccgggagcgc
     4141 aggccacggc gcgacaaggg tggcgcgacc ggaacggtcg ctgcaagccc ggggttttca
     4201 cgcatcggac cgtagcgaca gcccgaaacc gcgccagcgg gttcggcgga gactggcggg
     4261 tgcgccagca gctgcctgtc ggagtcgggc ctgacgaccg gatgccgcca ggcagccggg
     4321 agccgaaggt gcgcccggca gacagaacaa cactgcagta gtcagaatca gagcagcaga
     4381 taaaggaaca acaacatcag gaacagttcc ataacataag aaagaaacac tcctgagtca
     4441 gacacatcac agacgggaag ggtaacgggc gacgcgccag cgtggcccgc ttcccttccg
     4501 gcaggcgtca gcctgccagg ggttttcgcg cggactgaca ggcggcctcc accgcctgcc
     4561 cctgtatcac ctgtttctgc taccctgtaa gatgtagtga ccgcccagga aaaccgcctg
     4621 ctgattcgcc acaaaatcgc agatttcatc aacaatctgc gcggtggtga gcggtttctg
     4681 ccccatttca cgacgccgcg cattaatcac ggccctgtaa ttacgcagaa tttcgcgggt
     4741 ttctgtgctg atattcatga catcgtctcc ccgtccagcg cctgtaacac aacgtccatt
     4801 gcttcgcgtt cctccctgac gaatgcgtca ccgtcgcccc cgttctcact cccgacggca
     4861 tacaggcgct tcatttcact gcctgccagc gccatcactt tttccagcat caccgccggg
     4921 actgtcacct gacggctggc gcgcttttct tccatcagtt cacgaacaac gccgtgaaca
     4981 acgctgacat ccgcaccata ttcaaaggca tcgtccagcc gctgcatgac ggtttccagc
     5041 tcatcgtctg tcagactcca gtcctgggct atgtgacgca catccccacg ggtcagcact
     5101 acggcaaaaa cgcattcact gtccggcacg tctgtatcca gcacttcgcg gataatttct
     5161 ttaacagtac agtacatgtt ctcgtctcct ttacaggggc ggcattgccg ccccgtccgt
     5221 taatcagtcc agagccgcgt aaatcgcgtt gcattcgggg tgaaattcga tgtgacgcca
     5281 caactgcgca tcgcgcatcc ggtaaagctg ggtcagtcct gcatccccgc tgtcgtggta
     5341 cagccacaac tggcggttaa tcacaaggga ggtcaggatg atgcctgcgg catcagcact
     5401 cacggtacgg tcaaaccagt ttgcaccgtt caccatgtgg aaacggtcgc catccggcat
     5461 catgtaaccg ccaccgtccg gcaggcggat gaagtgccag aagcctccct gatattccgg
     5521 catcattttt tgcgccagcg cgtacacata cgcctcggca tacagaaaat cgttgccaaa
     5581 cagatacggc agaaaggaga gacggtcgca ctcttcaaca ttcagagtga caggtttcgc
     5641 atattgcatg atgttttcct ccgttgttca catattccgc gacaccgggc ggtgccgtgc
     5701 ctccataaaa gccgggcgaa caggcgggga tgcaggggcg caagggaagc cgcagcctga
     5761 cgcacacggc tgacgggagt tgcagggtga gaccgcgaaa tctgagcggg tgaacgaccg
     5821 gaaaatcacg gcagacggga aggagggacc gggtgaccgc agcggcgaag cgtacccttg
     5881 catcaacgcc agcccggcta agatggaggc acggcacagc ccgcacacga cgctgaggcc
     5941 acataactga aaaaccacaa ggccatgccc tgcatggccg ggtaaagcgg agccggcggc
     6001 gacttccttt gtgctctgtc cggggaccgg caggaaaaag gcggggattt accccgccag
     6061 tgtcatcatt tccggatttt tgccagggca tccgccatgc tgcggatttc ggtccgggct
     6121 tcccggacca cctcccgcag aacaccacgg tcaaaccagc ggcggggcgg ttcatccggt
     6181 atacgcagca catcgcaggc cagcccactg gacaggatat gctcaataca ctcactgacc
     6241 agatacattt ccaccgacga aaaacgtgct ccccggttca cgatggcatc gagcaccacg
     6301 ggcaggtttt cgtcatcttc atcgagccag caggggtgat cgctttcctt cacgaccagg
     6361 cgaaagcggc gcgtctggcg ggtcagcaac gtctgaccgg tggtggtcag cgccgcacgg
     6421 atacgccgcg catcttctgc cacctggtaa cagatgcttc gtaccgcatc gtgcaccgca
     6481 tcaagaacag ccctgatttc gtcttcggtc agccattctc cggcatacgg gaaagacacc
     6541 tgcgttccgt ctgccggaag taccggcagc gactgccact gactgccgga cagtaccagt
     6601 tccgccccgg ttccggtcag tctctgaatg gcggaggcca ggagcggcgg cagtacgccg
     6661 ccgtgacggt tgataagtaa cgtcgccagc cggggcgcat ccagccgcac gtaaatgctg
     6721 atccagcccg gatagttcgg atttcgctgg tcaacatccg tctcttccgg ccagccctgc
     6781 gccagcagca ggttacgggc gtgacgacaa atcagtgcgt tcagcgtgct gttttcgttc
     6841 atgattaagc gttctccctg tatcgccgcc cgcagggcag gcggcgataa catcagttgt
     6901 tttcgtgtgt gttgtttttc agcagggtgc gggcctgatt aaccaccgcc tgacggcggg
     6961 taacgcattc cgggacggcg aggccgttat cacgggcggc ccactcacgt ttccacagtg
     7021 cctgttcagc ggtctgaatc agggacgcca gcagttcagc agggacggtc acctggcggt
     7081 tttcgctcac gttgctgata atctccatca ccgcggcgga agaaataccg gattcagtcc
     7141 gctggtcttc cgggatgtcc tccatgcgcg ccaggacggt cctgatttca tgatcggtca
     7201 gagaaatttc cataccgtca gccagtgcct gaatctcttc cggggaccag ataaccagca
     7261 tcagcggcgc gtctcccgga tactttgctg ccagctcccg gcatagcgtt tcgcatgttc
     7321 cgtacattct cgtctccttc tcaatctttg aagcaggaat ccagttccag aatttcggta
     7381 tacgcctcca taaacaggcg attggcgcga tacagcgtct ggcgtgtcag gcgttcaaca
     7441 agcacatcgg aatcaaagcg acagcgatgc tccatatcgt tcagcctggc ccgaaggtca
     7501 cacatggtgt tggccacctc cgccacgtcg gcgcgaagtt tttcgtgtcc ggcatagttc
     7561 atttacgcag cctccggcat aaaccagtca tcattcgcgg ccccctgctg catggcccgt
     7621 tgcacggcgg caaggcgttg ctgcccggca cggtgatact gctcaagcag ctcgataccg
     7681 atataccggc gtccggactg gagggcggcg acgcaggttg agccgctgcc tgcaaacggg
     7741 tccagcacaa ttgcgttcgg gtgtgtgaag ctctcaatca gcggttgcag gctggtaacg
     7801 ggcttttccg tcgggtgatg acgattgcca gaatatttcc agcccagcac gtccggcagc
     7861 gggttttgtg gcagacgtgg acggcctttt gccaggatgt aggcgcattc gtggcgatag
     7921 cccacatatg cggccttcga tgtgtagttt ttggtgaaga ccaggtgacc aacaacgctg
     7981 aatcccgcat ttttccaggc ggccataaag cgatcgacgc ggttccagcc gtagaagctc
     8041 accatcagcg cgtctttttt gagtacgcgg tacatttcat tacaggccgg ttgcagccat
     8101 tcatcggttt tatcgccggc gatggtgcgc ccggaacggt cacgaaaacc gacgagatat
     8161 ggcgggtcag ttaatatgaa atcaatggcg ttatcaggga tacgcgtcat aacatcgatg
     8221 cagttaccaa ggacaaaacg ggacataaca acgctccttc tcttacagag ggcaccggga
     8281 gggcccccct gtggtgcccg ttccccgctg cctgtgggct gttcaccgcg tccggtaacg
     8341 ggtcaggccg accgtggaat atcgcagccg tcgtcttttt gacggcgaga atatgccgcg
     8401 aaagcggctt gaagggcgtt catcagaaaa gggcgacagg ctggcgtaca ctgtcaccgg
     8461 tgccagccgg gagcagtaaa agcgcccgtc ccgtgcccgc cggacgcggg acactgacca
     8521 caggcagcgt tcctgccgcg ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca
     8581 tcctttgtcg gaaaagaaac gggcggttgc ccgcccgtct ccgtcatact tcatcccccg
     8641 taatgcgcca ccttgtcgac tatccactcc ggcgcggtga cttccggcag ctcatcatca
     8701 tctgtcgggg aagaccattc cagttccccc cacagcacat caacaagctc tccacgctca
     8761 tatcgctgcc agccacagaa attgcagccc tgttcggcgt accagtgctc cagcgtgcag
     8821 ccgaaacggc ggcttaattc tgcaacaacg ttgctttccg gctgacacca cggcgtgtcg
     8881 aaatccactt gaatgaagtt ttctccctga cggctgatat tcacctcata ccccacaggc
     8941 cacttcacgc catactgttc cgtataccag tgatacgcag aaggaacacc gttcagaaca
     9001 ccaccgttaa agccattcac ttcaacatcc aggcgggtgg gcagaaccgt cagcaggtca
     9061 aacggcatgg ttttttccgg cagtacattg tcacacagac ggttccacca caccgataca
     9121 tcctcgttac tccagaagcc acaccagtca cctctttttg cggtgaaatg cacggtgatg
     9181 gtttccctga cctcatccgg taatccttcc cagcgacgct ggccagtacc actctgcagc
     9241 cagagttcat gcagcatcag gcagttttgc tcatccagca gtacaccgtt ctgcaggtgt
     9301 gtcagccagc gggtgaacgc gatattctcc ggcgatacaa caccacgtcc ggcatccgtc
     9361 agtccggggc acggttcaaa ctgcacatct tcagtggtct gcagaagtcc ggcacttccg
     9421 gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat aaagcggtgt gacggcaccg
     9481 ctggccagtc gtttaatctc agcaatctgt gccggttcac cagaaaaatg catacgattc
     9541 gagcaccagt ttggcatggg aattctcctc tcacatgcgg cctccatcag gccgtgccgc
     9601 acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga
     9661 ggaggaacga cgaaacgtga ccgggtgagg aggccggcga cagcgacaca cttgcatcgg
     9721 atgcagcccg gttaacgtgc cggcacggcc tgggtaacca ggtattttgt ccacataacc
     9781 gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat ccacagcagg catacaaccg
     9841 cacaccgagg ttactccgtt ctacaggtta cgacgacatg tcaatacttg cccttgacag
     9901 gcattgatgg aatcgtagtc tcacgctgat agtctgatcg acaatacaag tgggaccgtg
     9961 gtcccagacc gataatcaga ccgacaacac gagtgggatc gtggtcccag actaataatc
    10021 agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt
    10081 gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggacca tggtcccaga
    10141 ctaataatca gaccgacgat acgagtggga ccatggtccc agactaataa tcagaccgac
    10201 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
    10261 ggtcccagtc tgattatcag accgacgata caagtggaac agtgggccca gagagaatat
    10321 tcaggccagt tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt
    10381 agactaaaac gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa
    10441 ttttctctat acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc
    10501 ccttatacaa tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg
    10561 atgcagatga cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata
    10621 tcaccccagc ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt
    10681 tatctgtaaa ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag
    10741 aaaaaagagc aacaactgat ttaggcaatt tggctgtgtt gatacagcgg gtaataatct
    10801 tacgtgaaat attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca
    10861 atcggcttgc ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca
    10921 atctggataa tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac
    10981 tttcggtaag tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag
    11041 atactcttcg accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat
    11101 catccagtga gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag
    11161 aggtcttctc aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac
    11221 atacaggcaa agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat
    11281 ccaccatcgg ggcagctggt gtcgataacg aagtatctcc aaccggttga gtattgagcg
    11341 tatgttttgg aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag
    11401 acgatcgaaa atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc
    11461 agttgaagag cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt
    11521 accaacttca tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg
    11581 aatttgctcc tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa
    11641 aatacgtaca tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat
    11701 atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag
    11761 cacatcagca gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat
    11821 aactatgaca tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg
    11881 atcggtgggc agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag
    11941 agccagacag gaaggaataa tgtcaagccc aggccagcaa gtgggcttta ttgcataagt
    12001 gacatcgtcc ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag
    12061 atctggtacc catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag
    12121 caaaacacgt agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt
    12181 gtaaacgcca cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg
    12241 tcgcaatcgc gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac
    12301 acgttgctca acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc
    12361 ggcatctctg atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca
    12421 gcgccgggtt attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc
    12481 cttcgtcatt tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct
    12541 gaacatcctt taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc
    12601 aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact
    12661 acaaaaggag ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca
    12721 gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca
    12781 gatagctctt acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag
    12841 gtacacacgc ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga
    12901 tgacgaacta acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg
    12961 tgacaaactg ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa
    13021 tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca
    13081 aatgtgacga accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg
    13141 gaagtgatat cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca
    13201 atgtatgaga ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag
    13261 cggcggcgga agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc
    13321 cagtcgattt tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat
    13381 tcgtataaac gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc
    13441 gtaaaccggt tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg
    13501 taaagccctc tggatggact gtgcgcacgt ttgataaacc agggaaaaga ttcatagcct
    13561 ttttcatcgc cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc
    13621 ttcaatgcct gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca
    13681 tatttagcaa catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt
    13741 ctgatctggt caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc
    13801 gcctcagtga ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg
    13861 ataaccgctg tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca
    13921 cactaaataa aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga
    13981 gggcaatctg tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggta
    14041 atttgtcaca ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa
    14101 ggcctacaag gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa
    14161 tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca
    14221 gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgagggcttc gccctgtcgc
    14281 tcgactgcgg cgagcactac tggctgtaaa aggacagacc acatcatggt gctgtgttca
    14341 ttaggttgtt ctgtccattg ctgacataat ccgctccact tcaacgtaac accgcacgaa
    14401 gatttctatt gttcctgaag gcatattcaa atcgttttcg ttaccgcttg caggcatcat
    14461 gacagaacac tacttcctat aaacgctaca caggctcctg agattaataa tgcggatctc
    14521 tacgataatg ggagattttc ccgactgttt cgttcgcttc tcagtggata acagccagct
    14581 tctctgttta acagacaaaa acagcatatc cactcagttc cacatttcca tataaaggcc
    14641 aaggcattta ttctcaggat aattgtttca gcatcgcaac cgcatcagac tccggcatcg
    14701 caaactgcac ccggtgccgg gcagccacat ccagcgcaaa aaccttcgtg tagacttccg
    14761 ttgaactgat ggacttatgt cccatcaggc tttgcagaac tttcagcggt ataccggcat
    14821 acagcatgtg catcgcatag gaatggcgga acgtatgtgg tgtgaccgga acagagaacg
    14881 tcacaccgtc agcagcagcg gcggcaaccg cctccccaat ccaggtcctg accgttctgt
    14941 ccgtcacttc ccagatccgc gctttctctg tccttcctgt gcgacggtta cgccgctcca
    15001 tgggtatttt cagtgttgcc accatcgtct gcagctggct gacgtaccag gagtcagaga
    15061 gcggaaccag ccggtgagtc tgctgaccgg cgggcattct ccccgccgtc ctggcagctt
    15121 tttcggtccg ttgtttcagg gtcgcaagct gcacaaacgg atacggaggc gcaagcgaaa
    15181 aatccccccg cgtcagcgcc agtgcttcat taatgcgtgc tccggtgttc cacagtgtgg
    15241 ccagcagcat cttgcggtgc agatccggga cgtaatggag cagggcactc acttccggag
    15301 ccagcagata ttttggcagt tcatcatgga ccatcgacat ctggcgaagt gccagagctg
    15361 ccggataatc aatggcaaca ggcagcgatg caggctgccc ggcagaatac actgccggta
    15421 ccatgactgc agactggctg tgtataacgg agcctgacat ttatattccc cagaacatca
    15481 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
    15541 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
    15601 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
    15661 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
    15721 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagtccctg
    15781 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
    15841 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
    15901 cttaccagac cggagatatt gacatcatat gccttgagca actgatagct gtcgctgtca
    15961 actgtcactg taatacgctg cttcatagca cacctctttt tgacatactt cgggtataca
    16021 tatcagtata tattcttatg ccgcaaaaat cagcgcgcaa atacgcatac tgctatctgg
    16081 cttttagtaa gccttatgta ttttaccttt cgttatgtta accaataaaa attaaaatct
    16141 gacttataaa aacaaagcgt aattaccgca
//
LOCUS       DAPQZD010000109        15977 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPQZD010000109 DAPQZD010000000
VERSION     DAPQZD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.110"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            36..287
                     /gene="yefM"
                     /locus_tag="RO454_003324"
     CDS             36..287
                     /gene="yefM"
                     /locus_tag="RO454_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDY0935113.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKDIIE"
     gene            284..538
                     /gene="yoeB"
                     /locus_tag="RO454_003325"
     CDS             284..538
                     /gene="yoeB"
                     /locus_tag="RO454_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDY0935114.1"
                     /translation="MKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAACRYHY"
     gene            621..1445
                     /locus_tag="RO454_003326"
     CDS             621..1445
                     /locus_tag="RO454_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0935115.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1491..2420
                     /locus_tag="RO454_003327"
     CDS             1491..2420
                     /locus_tag="RO454_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0935116.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            2635..2697
                     /gene="yoeI"
                     /locus_tag="RO454_003328"
     CDS             2635..2697
                     /gene="yoeI"
                     /locus_tag="RO454_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDY0935117.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            2687..4045
                     /gene="plaP"
                     /locus_tag="RO454_003329"
     CDS             2687..4045
                     /gene="plaP"
                     /locus_tag="RO454_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDY0935118.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            4262..4720
                     /gene="tnpA"
                     /locus_tag="RO454_003330"
     CDS             4262..4720
                     /gene="tnpA"
                     /locus_tag="RO454_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HDY0935119.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKCRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(4831..6258)
                     /gene="sbcB"
                     /locus_tag="RO454_003331"
     CDS             complement(4831..6258)
                     /gene="sbcB"
                     /locus_tag="RO454_003331"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDY0935120.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            6467..7633
                     /gene="dacD"
                     /locus_tag="RO454_003332"
     CDS             6467..7633
                     /gene="dacD"
                     /locus_tag="RO454_003332"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDY0935121.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7752..8225
                     /gene="sbmC"
                     /locus_tag="RO454_003333"
     CDS             7752..8225
                     /gene="sbmC"
                     /locus_tag="RO454_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDY0935122.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQMVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            8423..9480
                     /locus_tag="RO454_003334"
                     /pseudo
     CDS             8423..9480
                     /locus_tag="RO454_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            9652..9981
                     /locus_tag="RO454_003335"
     CDS             9652..9981
                     /locus_tag="RO454_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDY0935123.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(10082..10264)
                     /locus_tag="RO454_003336"
     CDS             complement(10082..10264)
                     /locus_tag="RO454_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDY0935124.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVTSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            10318..10464
                     /locus_tag="RO454_003337"
                     /pseudo
     CDS             10318..10464
                     /locus_tag="RO454_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(10753..10866)
                     /locus_tag="RO454_003338"
     CDS             complement(10753..10866)
                     /locus_tag="RO454_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDY0935125.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(10879..11073)
                     /locus_tag="RO454_003339"
     CDS             complement(10879..11073)
                     /locus_tag="RO454_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935126.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(11070..11444)
                     /gene="cbtA"
                     /locus_tag="RO454_003340"
     CDS             complement(11070..11444)
                     /gene="cbtA"
                     /locus_tag="RO454_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDY0935127.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTSDNYRTVNNITLGKYPEAK"
     gene            complement(11533..11901)
                     /locus_tag="RO454_003341"
     CDS             complement(11533..11901)
                     /locus_tag="RO454_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDY0935128.1"
                     /translation="MSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(11917..12561)
                     /locus_tag="RO454_003342"
     CDS             complement(11917..12561)
                     /locus_tag="RO454_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDY0935129.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     RAVCGIFGIDLRQRVFRLSNGRFMEEACIWFSGFCSQGDGACFEGRWHWQPATARRLR
                     EYATQDHELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQAMT
                     ADAEAAVSEALRDLAFWLYRQLENEYDRLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(12580..12801)
                     /locus_tag="RO454_003343"
     CDS             complement(12580..12801)
                     /locus_tag="RO454_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDY0935130.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(12864..13340)
                     /locus_tag="RO454_003344"
     CDS             complement(12864..13340)
                     /locus_tag="RO454_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDY0935131.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(13356..13829)
                     /locus_tag="RO454_003345"
     CDS             complement(13356..13829)
                     /locus_tag="RO454_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDY0935132.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDYDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(13923..14168)
                     /locus_tag="RO454_003346"
     CDS             complement(13923..14168)
                     /locus_tag="RO454_003346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935133.1"
                     /translation="MQHVPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            complement(14168..14986)
                     /locus_tag="RO454_003347"
     CDS             complement(14168..14986)
                     /locus_tag="RO454_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDY0935134.1"
                     /translation="MRLTSRFGYVNQIRRDRPLTHEELMHHVPGIFGEDRHTSCSERY
                     TYIPTITVLESLQREGFHPFFACQTRVRDPDRREYTKHMLRLRRNGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(15207..15617)
                     /locus_tag="RO454_003348"
     CDS             complement(15207..15617)
                     /locus_tag="RO454_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935135.1"
                     /translation="MKTFIKTLLVAVTILFSVFAAAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKEQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(15633..>15977)
                     /locus_tag="RO454_003349"
     CDS             complement(15633..>15977)
                     /locus_tag="RO454_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935136.1"
                     /translation="YELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVT
                     INGTVWLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEF
                     AYLVKNYVAAVN"
BASE COUNT         3861 a         3842 c         4179 g         4095 t
ORIGIN      
        1 attgtacaat gaactgtaca aaagaggaga tttacatgcg tacaattagc tacagcgaag
       61 cgcgtcagaa tttgtcggca acaatgatga aagccgttga agatcatgcc ccgatcctca
      121 ttactcgtca gaatggagag gcttgtgttc tgatgtcact cgaagaatac aactcgctgg
      181 aagagacggc ttatctactg cgttcccccg ctaacgcccg gagattgatg gactcaatcg
      241 atagcctgaa atcagacaaa ggaacggaaa aggacattat tgagtgaaac taatctggtc
      301 tgaggaatca tgggatgatt atctgtactg gcaggaagca gataagcgaa ttgttaaaaa
      361 gatcaatgaa cttatcaaag atacccgcag aacaccattt gaaggtaagg ggaaaccaga
      421 acccctgaaa cataacttgt caggtttctg gtcccgacgc attacagagg agcaccttct
      481 ggtatacgcg gttactgatg attcactgct cattgcagca tgtcgttatc attattgaag
      541 agattcattc atagaatgaa ttgtcagttt tgatacgcta gcgtagcctt atagatctaa
      601 ggtgtatcag gagataacgg atgaaaaagg tcgcaattgt cgggttaggg tggttaggta
      661 tgccgctggc gatgtcactt tcagcgcgag gctggcaagt caccgggagt aaaaccacac
      721 aagatggtgt cgaagcggcc cgaatgagtg gcattgatag ctatctgctt cgcatggagc
      781 ctgagttagt ttgcgattct gacgatctgg atgcccttat ggatgccgat gcgctggtca
      841 ttacgcttcc ggcacgtcgt agcggccccg gcgatgagtt ctatttacaa gcggtacaag
      901 agttagtgga tagcgcgctg gctcatcgta ttccccgtat tatttttacc agctcgacgt
      961 ctgtctatgg cgacgcacaa ggcacggtga aagaaaccac cccgcgtaat ccggtaacca
     1021 acagtggacg agtattagaa gaactcgaag actggctgca caatttaccc ggtacttcgg
     1081 tcgatattct gcgtcttgcg ggcctggtcg gaccgggacg tcatcccgga cgcttctttg
     1141 ccggaaaaac cgcccctgat ggtgaacatg gtgttaattt agtccattta gaagatgtta
     1201 ttggcgctat cactctgttg ttacaggcac ctaaaggcgg acacatctat aatatatgtg
     1261 cgccagctca ccctgcgcgt aatgttttct atccgcagat ggcccgttta ctggggctgg
     1321 aacctccgca gttcagaaat agtctggaca gcggcaaagg taagattatt gatggcagtc
     1381 ggatttgtaa tgaactggga tttgaatacc agtatcccga tccgctggta atgccgctgg
     1441 agtaaacgat cacgccagca ggacgccgca tgcactgcaa gggggcgtgt atgaaaccac
     1501 tgctggatgt gttgatgatc ctcgatgcct tagaaaaaga aggcagtttt gcggcggcgt
     1561 cagccaaact ctacaagacg ccgtccgctc tgagttacac cgttcacaag ctggaaagcg
     1621 accttaatat tcaattgctt gatcgtagcg gacaccgcgc taaattcacc cgtaccggaa
     1681 aaatgttatt agagaaaggg cgggaagttc tgcataccgt gcgagaactg gaaaagcagg
     1741 cgatcaaact gcatgaaggc tgggaaaacg agctggtgat tggcgtggac gacacgtttc
     1801 ctttttctct gcttgcgcct ctcattgaag ctttttatca acatcatagc gtgacgcgcc
     1861 tgaagtttat caatggcgta ctcggtggtt cctgggatgc cctgactcag gggcgagcgg
     1921 atattatcgt tggcgcgatg catgagccac catcttccag tgagtttggt ttctcgcggt
     1981 tgggcgatct tgaacaagtc tttgcggtcg cgcctcatca tccgttagcc caggaagaag
     2041 aaccgttaaa ccgtcgtatc attaaacgat atcgggcgat tgtggtgggg gacactgcgc
     2101 aggcaggcgc ttctacggcc tcacaacttc tcgacgaaca agaagccatt accgttttcg
     2161 attttaaaac caagctggag ctgcaaatta gcggccttgg ctgcggctat ttaccacggt
     2221 atctggcgca acgttttctc gatagtggcg cgttaatcga gaagaaagtg gtcgcccaaa
     2281 ctctctttga acccgtctgg attggctgga atgaacagac cgcagggctt gccagcggct
     2341 ggtggcgaga tgaaatttta gcaaatagtg cgatcgccgg tgtttatgca aaatctgatg
     2401 acggaaaatc agccatttaa agaaaaatta ttatggcaag cctctcattc tcttgtcatt
     2461 tcccccccat ttaggcacaa tgcgccgctg tcaaaaaatg actaaaaacc gacgtttaat
     2521 tagcgtcggt tattttttgc ttcaaaccaa tcattcatac caagaggccg ggcttcgtac
     2581 cggatagata tttactaaaa atcgacagtt gttgtcgctg aggaatccaa aaaaatgggg
     2641 caattttttg cttacgcgac ggttatcacc gtaaaggaga atgaccatgt cgcataacgt
     2701 tactccaaac acctctcgcg tggaattgcg taaaacgctt acgttagttc cggttgtaat
     2761 gatgggtcta gcctatatgc agccgatgac gctgtttgat acatttggta tcgtttcagg
     2821 cctcacggat ggtcatgtgc cgacagccta tgcgttcgca ttgattgcga tcctgtttac
     2881 ggctctgagc tacgggaagc tggttcgccg ctatccttct gctggctctg catacactta
     2941 cgcccagaaa tccattagcc cgactgtcgg ctttatggtg ggttggtctt ctctgctcga
     3001 ctatctgttc gcgccgatga tcaacattct gctggcgaaa atctattttg aagctctggt
     3061 gccttccatt ccatcgtgga tgtttgtggt ggctctggtg gcctttatga ccgcctttaa
     3121 cctgcgtagt ctgaaatccg tagcgaactt caacactgta atcgtcgtgt tgcagatagt
     3181 gctgatcgcg gtgattctgg gcatggttgt ttatggcgta tttgaaggtg aaggcgctgg
     3241 tacgctggcg agcactcgtc cgttctggtc tggcgatgcg catgttatcc cgatgattac
     3301 cggggcgaca atcctgtgct tctcctttac cggttttgac ggcatcagca acctgtcgga
     3361 agaaaccaaa gatgcagagc gcgtgatccc gcgtgcgatt ttcctgaccg cgctgatcgg
     3421 cggcatgatc ttcatctttg caacttactt cctgcagctg tacttcccgg atatctctcg
     3481 ttttaaagat ccggatgcgt cacagcctga aatcatgctg tacgttgcgg gtaaagcgtt
     3541 ccaggttggc gcgctgatct tctccaccat taccgtactg gcgtccggta tggcggcgca
     3601 cgcaggcgtt gcgcgtctga tgtacgtaat gggtcgtgac ggcgtgttcc cgaaaagctt
     3661 cttcggttat gtacacccga aatggcgtac tccggcgatg aatatcatcc tggttggcgc
     3721 gattgccctg ctggcaatca acttcgacct ggtaatggct acggcgctga ttaactttgg
     3781 tgcgttggtg gcgttcacct tcgttaacct gtcggtcatc tcgcagttct ggatccgtga
     3841 gaagcgtaac aaaacgctga aagatcactt ccagtatctg ttcctgccga tgtgtggtgc
     3901 gctgaccgtt ggtgcgctgt gggttaacct ggaagaaagc tcaatggttc tgggtctgat
     3961 ctgggctgct atcggtctga tttacctggc ttgcgtaacc aagagtttcc gtaacccggt
     4021 tccgcagtac gaagacgtag cgtaatctta caaaccggat gacataatca tccggtaaat
     4081 ctcaaaaagc cggagtttgt cgctccggct tttttgttgt gcacagaaaa cccccagcta
     4141 ggctgggggt tccggaaagc tttcagcttt gagccagtta ttaaaacccc ttttgatttg
     4201 ttaaaacacc ttgcggtctg gcaactgcaa gtgtcaaaca agaaatcaaa agggggtccc
     4261 aatggggaac gaaaagagct tagcgcacac ccgatggaac tgtaaatatc acatagtatt
     4321 tgcgccaaaa taccgaagac aggtgttcta cagagagaag tgtagagcaa taggctgtat
     4381 tttgagaaag ctgtgtgagt ggaaaagtgt acggattctg gaagctgaat gctgtgcaga
     4441 tcatatccat atgcttgtgg agatcccgcc caaaatgagc gtatcaggct ttatgggata
     4501 tctgaaaggg aaaagcagtc tgatgcctta cgagcagttt ggtgatttga aattcaaata
     4561 caggaacagg gagttctggt gcagagggta ttacgtcgat acggtgggta agaacacggc
     4621 gaagatacag gattacataa agcaccagct tgaagaggat aaaatgggag agcagttatc
     4681 gattccctat ccgggcagcc cgtttacggg ccgtaagtaa cgaagttgga tgcaaatgtc
     4741 agatcgtgtg cgcctgttag ggcgcggctg gtaagagagc cttaaaggcg catttgaaaa
     4801 acctccggct atgccggagg atatttattt tcagacgatc tcttccgcgt actgccaaag
     4861 cgcttttaac agcgccactt tctctttgtc atcggcatac tgttgtgcca gcatctgcaa
     4921 ttcatcagca tagccctgca aaaactctgg cgtgaagacc tggcggcggt gctccagcca
     4981 gcgttgctgc tcggcataat ccagcgtccc cgggaagttg cgcgctcgat aattgaacaa
     5041 cagtttttca attcgtttat caacaaaagt gatatccagt gccggtaaat tacgcggctc
     5101 ggtttccagc acaattttca ttgctgcacg atcggcgtca ctgaaaaagc cattataaag
     5161 ctgtgcatcc acgttatctg aaggtgtaaa cggttcggct tccgcgaata tcgccaccac
     5221 tttttcacgc acttgcggat tttcacgcag aatttttagg ttatcgaggc aatgctgacg
     5281 attaattccc agtcggtcgg catcttccgg gcgtagcgta ttcgcctggg ccagtaccgg
     5341 acatttattg atatgcacca gcttaaccgg aacggcggcg ttatcgccaa gatcggcttt
     5401 tgcggtatat aaacgctcgc gcaatgtgtc gctatccagc tccagtaatg gcgaaatgtc
     5461 tcctgccaaa tccaccaaaa ttacggcatt gcgattttca ggatgccacg ccagcggtgc
     5521 cacccagctg gtattgccac gccatgcccc aaacattccg gaaacgtgaa ctaggggttt
     5581 catctgcgga acatcaatca acgccatcag tttgtgttta ttacgatggg taaagagata
     5641 atcaaacagg cgtggctgac gcgtttttac cagtttcgcc atcgcaatag tggcgtacac
     5701 atcagccatc gcatcgtggg cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc
     5761 aaggcgaaag ctcggtagac cgtcatcatt ttcaggccag tttattcctt ccgggcgcag
     5821 ggcataacag gcacgcataa catccagtaa atcccagcgc gagttatcat gctgccagct
     5881 ccaggcgtaa ggatcgtaga aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa
     5941 acgcacattg ttgtagccca gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg
     6001 ggcggcaaac gcggcttcgt tttctccttt cgcccgtgcc tcctgcgggg taataccggt
     6061 aattaatact gctccaggct ggggtaaata gtcatccgcg ggcttgcagt aaaagacttc
     6121 gggttcgccg atgacattga attcgttatc agtgcgaatg gctgcgaact gtgcagggcg
     6181 atctaacgcg gggtgcgttc caaaggtttc gtaatcgtga aacaaaaagg tagattgttg
     6241 cttaccgtca ttcatcatta ggttaaatcc gttatttctg ctgtctgcca gagtatcaaa
     6301 tatcaccgtg ctaatcagct ttagcgcgac aatttggcag cgagtggcaa cagatcatgt
     6361 cagataaaaa tgagatagta gtcacatttt cttgcacttt attccagcca gttcataagt
     6421 atttccgtaa aaagaacagc tatttgaaac acctgagggt ttgctgttga aacgccgtct
     6481 tattattgct gcttctttgt tcgtttttaa tttatcgtct ggttttgcgg cggaaaacat
     6541 tcctttttca cctcagcctc cagagattca tgccgggtcc tgggtattga tggattacac
     6601 caccggtcag attctcaccg cgggtaatga gcatcaacag cgcaatcccg ccagcctgac
     6661 aaagctgatg acgggctatg tcgtggatcg cgctatcgat agtcatcgca ttacgccaga
     6721 cgatattgtc accgtggggc gtgatgcgtg ggcgaaagat aatccggtgt ttgtcggttc
     6781 ttcactgatg tttttgaaag agggcgatcg cgtatcggta cgtgatttaa gccgtggttt
     6841 aattgtggat tccggaaatg acgcttgtgt tgctctggct gactatattg ccggtgggca
     6901 acggcagttt gttgaaatga tgaacaacta tgccgagaag ctgcatctca aggatacgca
     6961 ttttgaaaca gtgcatggtc tggatgcacc tggccagcat agctcggctt atgatttagc
     7021 cgtgctttct cgcgctatca tccacggcga gcccgagttt tatcatatgt acagtgagaa
     7081 aagcctcacc tggaacggta tcacccagca aaaccgtaac gggttattgt gggataaaac
     7141 catgaatgtt gacggcctga aaacgggcca tacttctggt gccgggttta atcttattgc
     7201 ttcggctgta gacgggcagc gtcgcctcat tgcagtggta atgggggctg acagcgcaaa
     7261 aggtcgtgag gaagaggcaa gaaaattact gcgttggggg caacaaaact ttactacggt
     7321 gcaaattttg caccgtggga aaaaggtcgg tacggaacgc atctggtatg gtgataaaga
     7381 aaatatcgcc ctgggaacgg aacaagagtt ctggatggtg ctaccgaaag ccgaaattcc
     7441 acatatcaaa gccaaatata cccttgatgg taaagaactc accgcgccaa ttagcgccca
     7501 tcagcgggta ggggaaattg aactttacga ccgtgataaa caggtggcgc actggccgct
     7561 ggttaccctg gaatctgtcg gggaaggcag catgttttcc cgcctgagtg attatttcca
     7621 ccataaggcc tgacctttct tttgcagcag actggcagga gtgcgagtct gctcgcataa
     7681 tcaacactca ttccttgtgg ttttaatatt gcaactatac tgtatataaa aacagtgtta
     7741 gtggaggcgt catgaactac gagattaagc aggaagataa acgtaccgtt gcaggtttcc
     7801 atcttgttgg cccgtgggag cagatggtaa agaaaggttt tgagcagttg atgatgtgga
     7861 tagatagcaa aaatattgtg ccgaaggagt gggttgctgt ctattacgac aatccagatg
     7921 aaacacccgc cgaaaaatta cgctgcgaca ccgtcgtgac ggtaccgaat aactttacgc
     7981 tccccgaaaa cagtgagggc gtcattctga cagaaatttc aggtggtcag tatgcggtgg
     8041 cggtggctcg tgtagtcggt gatgattttg ctaaaccctg gtatcagttc tttaatagcc
     8101 tcttgcagga cagtgcttat gaaatgttac caaagccctg cttcgaggtt tacttgaaca
     8161 atggcgcgga agatgggtac tgggatatcg aaatgtatgt tgcggtgcag ccaaaacatc
     8221 actaattcat ctcagggcgg tgtgttaacg cgaagaccac tctttttttg aaagcgaaaa
     8281 gagtaagatg cgcctttcaa atttttcgct cctgccggga aattacactg ttcccggtat
     8341 gtccgtcgga taactcagag gcgcgccttc tggccgacag atgtgttatg agcgcttttc
     8401 atcttcatta cggagtttct gcgtgcgtgc cgataagtca ttaagcccgt ttgaaatccg
     8461 ggtataccgc cattaccgca ttgtgcatgg tactcgggtc gcgctggcat tcctgctcac
     8521 tttctcatta tccgcctgtt tactatcccg gaaagcacct ggccgctggt caccatggtg
     8581 gtgattatgg ggccaatctc gttctggggg aacgttgtcc ctcgtgcctt cgaacgtatt
     8641 ggcggtacgg tgttgggttc gattttaggt cttatcgctc tgcaactgga gttaatttcg
     8701 ttaccgctga tgttagtctg gtgcgcggcg gcgatgtttc tttgcggttg gctggcgctg
     8761 ggcaagaaac cgtatcaagg attattgatt ggggtgacgc tggcaattgt tgtgggttcc
     8821 ccgacaggtg aaattgatac ggcgttatgg cgaagcggcg atgtgatcct cggctcttta
     8881 ctggcaatgt tgtttaccgg tatctggcca caacgggcgt tcatccactg gcgcattcaa
     8941 ctggcgaaaa gtctgaccga gtataatcgg gtctatcaat ctgcattctc accgaactta
     9001 ctcgaacgcc cacgtctgga aagccatcta caaaaactcc tgaccgatgc cgtgaaaatg
     9061 cgtgggctga ttgcgcccgc cagcaaagaa acccgtattc ccaaatcgat atatgaaggt
     9121 atccagacca ttaatcgcaa tctggtttgt atgctggagt tgcaaatcaa tgcatactgg
     9181 gccacgcgcc ccagccattt cgtgttattg aacgcgcaaa aacttcgtga tacccagcac
     9241 atgatgcagc aaatactgct gagccttgtt catgcgctgt acgaaggtaa tccgcagccg
     9301 gtttttgcca atacggaaaa attgaacgat gctgtggaag agctgcgtca gttgctcgat
     9361 aaccaccatg acctgaaagt cttggaaaca ccaatctatg gttatgtgtg gctgaacatg
     9421 gaaacggcac atcagcttga gttgctatcg aatctgattt gccgggcctt gcgcaaataa
     9481 ttcctgaact tcagaatcat cttgctgctg cttcgattca gcaaggataa agggtatgat
     9541 agtgaaaagg gataaaagca ttgtcatctg cggcagctat gagtaatgtt ggccctaacg
     9601 aatagcggtt gcttaaacga atccgactct cacattatca ggggtataaa aatggaaact
     9661 accaagcctt cattccagga cgtactggaa tttgttcgtc tgttccgtcg taagaacaaa
     9721 ctgcaacgtg aaattcagga cgttgagaaa aagatccgtg acaaccagaa gcgcgtcctg
     9781 ttgctggaca acctgagcga ttacatcaag ccagggatga gcgttgaagc aatccagggc
     9841 atcatcgcca gcatgaaagg tgactatgaa gatcgcgttg acgattacat catcaaaaat
     9901 gccgagctct ccaaagaacg ccgcgatatc tccaaaaagc tgaaagctat gggcgaaatg
     9961 aaaaacggcg aagcgaagta attcccgttt tattcaatga gggttgcccg gcaaccctca
    10021 ttgctcattg attcttatct gtgtatcacc gtcatcattc tcatctgaga accaatcgaa
    10081 attaacaaca gccttcttct gtatgcagca aggcaaaaag ttctgtaact ccattgttat
    10141 taactgcact ggttactaac acgttgtgcg ctccagcttc ccgtaaccaa cttgtcacca
    10201 aagatatttg ttccatgctg gctaaatctg ctttggttac tactccaatg accgggtgat
    10261 tcatggcccg gtagggcgtt tttacctcta attcctgctt cagcgctgag attcctgctt
    10321 gttgccggaa aagcaggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc
    10381 catattctga gcatactgcc ggaatggccc tccaaccgtg ttgacgaact cctgccatgg
    10441 aacgtagttc tcaccaataa ataagcgtca atacggtgct ccgttgacgc ttactttaat
    10501 ccgttttctg agcttagatt tttctgttcc tacccagacc gtggatttgg aattcatttt
    10561 ttgtcaggtc aatgctaatc gttaaaacgg ccataacgca cctccactgg aaggtttatc
    10621 agtaatatcc taccgttgct tcatggtcgg ggcgtccatt ccattattat cggttttcct
    10681 ttctgtttat aaatgaaaac gccagctgta ttcaggctgg cgtcagggga aatgaagcct
    10741 gttgagtgag attcaccggt tctggtgcag aagctgcaga tggcgcaggg taaggacatc
    10801 ttctcttgcc tgcttaatac aggacaacag tgctgtttct gtggttaatg gcactatacc
    10861 ctgtatcact gcattgtctt acagcaactg gtgagggctg gcataagttg ccggtgtttc
    10921 ggtagagtca cggtcatcca ccacgcggag tgaataaccg ttttcagcgg ccttattaat
    10981 cagttcagtg atattaacac catcaatgtc aacgacaatg tgccccatat ccagcgcctg
    11041 tacgttaacg ctgtcggctt ccagtgtcat catttcgcct ccggatactt acccagggta
    11101 atgttattta ccgttctgta attgtcgctg gtcatcaggc cggtcgccct gcgagcccgg
    11161 aggatatcga tgctgtttat taactgagag cgggtacagg cgctgaatcc cggctggtcg
    11221 gtacgcacca gcgcgtattt ttccacgaga aagttcaccg catcacacag tgaaatgcct
    11281 gcctcaatat gctgctcaat cacacgttca tcggcaaacg gtgtgtcatt cagtgtgagg
    11341 ccatagtgct ggtccagcag tcgggacagc agtatctgcc agatttcaac aggagacggg
    11401 cgagaactgg ccgcctgccc gggtaataca ggtaatgttt tcatactgaa gattttcctg
    11461 atatgcagat ataaaaatgg aaaagtggcg tggtgaaaac accaggccgt agcagaaggc
    11521 tattctggag agttaatttt tcatttcggg cgtcggataa acagccagat aaacgtaacc
    11581 acaactgctg agggtatcgg ctttgcaggt cagccctttt gcatacagcg tgacggtatg
    11641 ctgatggcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag
    11701 cggaaaggcc tggtccaggt ggtacgcatc tgcatcgctg aacaggcctc tgataccggc
    11761 gcggtcggca aggtaatgca accggttacc ctcctgcacc agacgtgccc cgaaacaggg
    11821 cgtcacggtg cagggcagtc cccaccagag gcggtcgtta tggtcgtcgg gataatttgt
    11881 ctcatggagt ttgtctgaca cgataaagtc ctcaggtcag ccggcgcgca gaccggcttc
    11941 ggtgaaggtg tattcgttga tgagaagggc ctcatccaca gcggcatcag aggtcagcct
    12001 gtcgtactcg ttttccagct gacggtacaa ccagaaggcc aggtcgcgca gggcttcgct
    12061 gacagcggct tccgcatcgg ctgtcatagc ctgaccagtc ggactgttcc gggtaacagt
    12121 gatatccatg ctgtacggat ggcagtaacg tccccggtgg tggatttcag cctgcagttg
    12181 ccagaagtta cgtttctgca ccgcctgcag ggcatccgcg atacgatgga gttcatggtc
    12241 ctgtgtggca tactcccgga gccttctggc tgttgctggc tgccagtgcc agcgtccttc
    12301 aaaacaggct ccgtccccct ggctgcagaa tccgctgaac cagatgcacg cctcttccat
    12361 aaacctgccg ttgctgagcc ggaaaacgcg ctggcggagg tcaattccga aaatgccaca
    12421 gaccgccctg aagtcctcat acacgttttc ataccagttg ctgtcagcat gatgctgacg
    12481 gtaccagtcg cgggcttttt cctgtgcggt ggaggatagc tcatgcagtg tatacacggt
    12541 ggtggtgata attctgctca tcagaatgcc cttactgact cagttcagag tgacgctcat
    12601 cagccggaca tacgggccaa aactgtcctt acggcgttca gcaaacacgg ccagcacacc
    12661 gggaatatcc tgcacttcac gaccggtata cgcttcagca ctgccgtgcc agcggtattt
    12721 gccggtacag aacggaaaaa gacgggatgc aggatgctga cggtgaatac gcatggcttc
    12781 gccacgggtg atgattttca tagtgggata cctctgaaga cagaagataa aagtgaaaac
    12841 aggtgtgatg tggttgtgac gggttaaagc agaccatgtt cggcaaagga gaaaacctgg
    12901 ctgccaccga ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc
    12961 agacgttcgg tgataagccg gtctgcctta ctgggtgtga cttcaccgga cgggtgattg
    13021 tgcgccagca ccacggctgc ggcattgtga tacagggcgc gtttaatcac ttcccgggga
    13081 tggacttccg tgcggttgat ggtgccggtg aagagggttt caccggcaat cagctgattc
    13141 tggttattca gatacagcac ccggaattct tcacgctcca gtcccgccat gttcagaatc
    13201 agccattccc gtgccgcacg ggtggaggtg aaggccacgc cgggttcatg aagatggcgg
    13261 tccagggttt tcagggcccg ctgaatgagg ctgcgctctc tggttgtcat ttctccgggc
    13321 agaaaggaaa tctgttgcat tgttccggcc ccctttcagt caataatgcg cataatggcg
    13381 ctgcattccg gatgctgcag ggcgtaatcc cgcagacggt aataatgaac cgtcatggca
    13441 taacactccg tacggcaggc gtgatgactg tacgtcatca gacaggcggc aatgccggcg
    13501 gcttccgggc tcatttcagc gcggttaccg ttcatggcat tgaacagtac ccatgtttcg
    13561 tcatcatagt catccggttc aggggccata aatgccccgc cgttgttcag ggtgtacaga
    13621 ttccagatac caccgcagta gttttcgcac agacggtcca tccagccgaa gacacggggc
    13681 tccagggtca cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt
    13741 tcatcaggga ctatggtggc aaccagttgt tgcaggccag tctccggtgc acaggccgaa
    13801 gaggtggtgt tctgagacaa tgttttcatg tatgaaatcc ttgtaatgtg aataaatgag
    13861 agggagtaaa ggtcgggagc tggcaacatc acgttcatca ctgaccgtga gacccggtaa
    13921 tatcagggat gaatgatatt cggctgaaat tccggacagc cgtacgcgta accgttccgg
    13981 tcacagacgg atggccagat gccattgcct gtactcagtc tgcacagact gatttctccg
    14041 ggacggtaaa gcagacgggt acaccaggcg cacaggtcat catcagaaat ggaacgacac
    14101 cccggcagcg cctccggggc gtcgggtgat tctggtgcct gaatcacgcc ttcagggaca
    14161 tgctgcatca gcgcaggctc tccagcagcg tttctgccat tacccacaat gcgcggttga
    14221 gcttaatatc ggtgtcgatg ctgtgaatgg cacgggtgtg gatacgtttt ccttttgcac
    14281 tgcgaccgga aatcccgcct ttcagcatat tctcctggat ggtctgataa gtactccaca
    14341 ggtccttacc gtaatcctcc cggcgtcgtg gcgtcagaat gtcggcggtg gtgacgggcc
    14401 gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg
    14461 gcggcagaat cagcgactgc atggcatcac gcttctcctc tatccggtca aacacgccca
    14521 ccacctcgta agccccttca atgactctgt ccaccacatt tccccggtgc ggaacacgca
    14581 cttcccccag agactgacca cagacgcatc cgttctggca gacgaacctg aagtaacccg
    14641 gcagcatctg gtagctggag gtaccgtcat gagagttgag cagaataatt tcagggacat
    14701 gttctccgtt tatctctccg ttacggcgca gacgcagcat gtgtttggtg tattcccggc
    14761 ggtccgggtc acgtacgcgg gtctggcagg cgaagaatgg atgaaagcct tcccgctgca
    14821 ggctttccag tacggtgatg gtggggatgt acgtatagcg ttcactgcag gaggtatgcc
    14881 ggtcttcacc gaaaataccc ggtacatggt gcatcagttc ttcgtgtgtc agcggacggt
    14941 cacggcgtat ctggtttaca taaccaaaac gactggttaa tcgcatgatt tgctccttat
    15001 cggtggttaa gatttactgg tgtaataaat gaaaaaagcc acgtctcccg gagaagacgc
    15061 ggcctgacgg gtgaaatgaa tgaggtttat tgtctgagaa gcctttcact ggcgagccac
    15121 gcattaagcg gctttccggc aacaatggct ggggctgtat ttacctttga tattaataat
    15181 gtgcagagaa acgcatggtt tactcctcag gttttaagtt tgtccagagc cacaacgaca
    15241 ggttttccgg atgtgtcgat gactcctacc gtcggtacgc catttttgat ttcgaagaac
    15301 ggtttgttgc tacctgagca cagctggttg tctttgtatt cgagcatctg ttctttcccg
    15361 gttttcaggt taagtaccga gacagtgtag tcgtaacacc aggcaccacc acgttcaagc
    15421 acgaaaacat agctgttatc tttatagttg aacggattct tcgtctggca attcataccg
    15481 tcaatctcag tacaggaaaa cgcctctgta gtattaacgt atttgatgtt gtttggcagt
    15541 tttacttgtt tcgccgcagc gaagacagag aacagaatag ttacagcaac gagtaaagtt
    15601 ttgataaaag ttttcatgac agactcctta aattagttaa ccgcagctac gtagtttttc
    15661 accagatacg cgaattcatg cacgccaatt tcgccgaacc aaaccaggta ggccgggtca
    15721 tctaaaccaa agtagcggta gccaacgact tcatgggttt ttgggttata gagtgttacg
    15781 ataaagtccc agccgcaggt attggctttg caaccgtcgc cctggagcca gactgtaccg
    15841 ttaatggtta ctggcgttac gtttccgcca gggccatcca tatacccgcc tttaccgaac
    15901 atgccgctca gtccgaccgg ttttaatgct ttgtcaagcg cagctttgta ggcagcgtca
    15961 ccttcgtaca actcgta
//
LOCUS       DAPQZD010000110        15833 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPQZD010000110 DAPQZD010000000
VERSION     DAPQZD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.111"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(18..743)
                     /gene="phoU"
                     /locus_tag="RO454_003350"
     CDS             complement(18..743)
                     /gene="phoU"
                     /locus_tag="RO454_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDY0935137.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(758..1531)
                     /gene="pstB"
                     /locus_tag="RO454_003351"
     CDS             complement(758..1531)
                     /gene="pstB"
                     /locus_tag="RO454_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDY0935138.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="RO454_003352"
     CDS             complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="RO454_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDY0935139.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="RO454_003353"
     CDS             complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="RO454_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDY0935140.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="RO454_003354"
     CDS             complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="RO454_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDY0935141.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="RO454_003355"
     CDS             complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="RO454_003355"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDY0935142.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="RO454_003356"
     CDS             complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="RO454_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDY0935143.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8626..9045)
                     /gene="atpC"
                     /locus_tag="RO454_003357"
     CDS             complement(8626..9045)
                     /gene="atpC"
                     /locus_tag="RO454_003357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDY0935144.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="RO454_003358"
     CDS             complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="RO454_003358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDY0935145.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="RO454_003359"
     CDS             complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="RO454_003359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDY0935146.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="RO454_003360"
     CDS             complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="RO454_003360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDY0935147.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="RO454_003361"
     CDS             complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="RO454_003361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDY0935148.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="RO454_003362"
     CDS             complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="RO454_003362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDY0935149.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="RO454_003363"
     CDS             complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="RO454_003363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDY0935150.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="RO454_003364"
     CDS             complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="RO454_003364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDY0935151.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="RO454_003365"
     CDS             complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="RO454_003365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDY0935152.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3795 a         4314 c         3963 g         3761 t
ORIGIN      
        1 gggatttact tggtgaatta tttatcgcta tctttttctg ccagcagttt atcgagctcg
       61 tcaccaccga cgtgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata
      121 ttctggcagc ggtcgccaat acgttcgata gaacgcgcac agaataacgc ggtaagtacg
      181 ctcggaatgg tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
      241 tactcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
      301 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
      361 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
      421 ttatccgcca cgtcaccaat acgctccagc tcggcaatgg ttttactgat taccatcacc
      481 agacgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
      541 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tgacgcgctt cgccagatcg
      601 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
      661 atggtcatca cctgcgtgcg gatgctttcc agttcggcgt tgaactggcc ggaaatatgt
      721 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
      781 agtcttctgt ttgttttttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
      841 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
      901 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
      961 gcccggtaga gatagggtcg agtgccgaac acggttcgtc gagcagcagc acttccgggc
     1021 gaatggcgat accacgcgca atgcacagac gctgttgctg accaccagag agagagtaac
     1081 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
     1141 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgcacg ccaaaggcga
     1201 tgttgtcgta aatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
     1261 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatcg agcagaattt
     1321 caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
     1381 ttgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg gcgatatcca
     1441 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
     1501 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tgcctgatgc
     1561 acttcgcgaa tcaggcttac cgtaggccgg ataaggcgtt tacgccgcat ccagcaaagt
     1621 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcagtacgc
     1681 acagggtaat gatcaacacc ccggcccagg ccagttgctg ccattccgca aacgggctca
     1741 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
     1801 agaactggtt ggagagcgca gtaaacagca gcggtgcggt ttcaccagca atacgggcaa
     1861 tcgccagcag gataccggtc atgatcccgg acaccgacgc tttcagcgtg atcgaggcga
     1921 tcatcttcca cttcggcgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
     1981 gtttcagcat gttctcggtg gtgcggataa caatcggcac ctgcaacagc gccagcgcaa
     2041 tcacgcccgc ccagccggag aagtgctcca tctgcgccac cacgatggtg taaacaaaaa
     2101 gaccaaccac aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg
     2161 ccagccagga tttacgacca tattcagcca ggtaaatccc cgccataatg cccagcggcg
     2221 taccgaatac cgtagcccac aaaattaaca ggccgctacc cgccagggcg ttcgccagac
     2281 caccgccttc cgtattgggt ggtggcgtca tttcggtgaa cagcgccagc gacataccgt
     2341 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacaga ccgaaggcca
     2401 tcgtcgccat tgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt
     2461 tgcggcggga ttcagccagt gccgcagtgg tttgcatttc aaccatagcc attagcgtgc
     2521 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
     2581 aatcacaaac aggatcaggc ccagttccat cagtgcagca acgtgcagac ccgactctgc
     2641 ttcggcaaat tcgttcgcca gcgcagaggt gatactgttg cccggcatgt acagcgaggc
     2701 gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccaagcgc
     2761 gcggcccagc cccaacataa tgccgccgat aacaccattt ttggtgaacg gaagaacgat
     2821 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
     2881 cggggtttgt tcgaacacat cgcgcattac cgccgcaatg tacggaataa tcatgatggc
     2941 gaggatcacg cctgccgcga ggataccgat accaaaggcc gggccggaga acagcgcgcc
     3001 gacaatcggg atattcgaca tgatattgcc gaccggctcc tggaagtaaa cggcgaacag
     3061 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag
     3121 ctcaatggcg atacccagcg ggcgcttcag ccagccaggc gcaagctcgg tcaggaacag
     3181 ggcgataccg aaactcaccg ggacggcgat cagcagcgcg ataaacgagg tcaccagcgt
     3241 accgtagatc ggcaccagcg ccccgtagat atcgttcggc gcatcccact ctttggtcca
     3301 caggaaagcc agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat
     3361 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
     3421 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
     3481 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta
     3541 cccggcctga gttttattag tacagcggct tgccgctact gtctttaata ttggtcttcc
     3601 acgcagcgcg aacctgttcc actacgctat ccggcaggct ggcgtaatcc aggtcgttcg
     3661 cctgtttagc cccggttttg tatgcccagt cgaagaactt cagcacttct gtgccttgtt
     3721 ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata ggccacgcat
     3781 cttcgccttt ctggttggtc agatcctgag cgaatgtttt gctccagtct gcgccttttg
     3841 ctgcattagc gaagttttct tcggtcggac taaccggttt gccatcagcg gagatcaatt
     3901 tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacgtaacca attgcacccg
     3961 gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga ccgatcggcc
     4021 attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccaggt
     4081 agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca gcaatgttct
     4141 gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc cacttcttga
     4201 ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca
     4261 gacctggaat attaaccgcc agcaccacgc cgccaatcac ggtcgggaac tggaacaggc
     4321 cttcctgagc cagtttttca tcagacagcg gcgcgtcaga ggcaccgaaa tcaacggtgt
     4381 tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac
     4441 cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac
     4501 ctgcacctgt caggcttgct tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg
     4561 cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata
     4621 aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatgtaa
     4681 ggtttatgac agttttatga cagagagaga aagtctttag tctgatttaa ataagcgttg
     4741 atattcagtt aattacaaat attaataacg aagagatgac agaaaaattt tcattctgtg
     4801 tcagagaaaa agtagccgaa gatgacggtt tgtcacatgg agttggcagg atatttgatt
     4861 aaaaacataa caggaagaaa aatgccctgc ttacgcaggg catccattta ttactcaacc
     4921 gtaaccgatt tcgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg
     4981 tggtaagcca gtagttgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
     5041 tgcggcatct caatgatgtg catgttgtca ctgctcacaa aacccgcatc ctgatcggcg
     5101 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
     5161 agcaattcgt tgttcggtgc aacgacgata accggcatat cggcatcaat cagcgccagc
     5221 ggaccgtgtt tcagttcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
     5281 agcttcaatg caccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
     5341 tgatgtttgt cagagaaatc ttctgccagc gcttcaatgc gtttgtcctg agacagcatc
     5401 tgctcaatac ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca
     5461 tccagacctt tcaggcgaga cagtttcgcc accagcatca acagcacagt taactgggtg
     5521 gtgaatgctt tggtggaggc cacgccaatt tccgtaccgg cgttggtcat cagcgccaga
     5581 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
     5641 cccagctctt tcgaaagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
     5701 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
     5761 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
     5821 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttca
     5881 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     5941 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg gcggtaaata
     6001 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     6061 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
     6121 aggaagataa aacgacgagt cactggcaac agcgccagct ggtcagaagc gataaagttt
     6181 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
     6241 ggatgacggg agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc
     6301 agaactgcct cacgcagagt accgccttgt ttcagctccc agttcaccaa atgtgcaatc
     6361 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
     6421 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
     6481 tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg
     6541 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctgtacttt accgaggcga
     6601 cgcaggcggg tcatatgacc ttcagcatcc accacggcca ggccggcaga gtcatatccg
     6661 cgatattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
     6721 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     6781 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     6841 gcttgtgggc gggagattat gttatctcct catcccatat gaccggatta cccgaccaga
     6901 attacttttt ctttatcgga cgacgccagc cttctttttg agtctgcggc acacggctga
     6961 tagccagggc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
     7021 ctttgcctac tgttaccggg gccaccagtt gagtgtcgga accgacaaac acatcgtcgc
     7081 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcaccga
     7141 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
     7201 ctttacccag acgcgctttt ttcatctcaa cgaagttacc gacgtgtgca ccttccagca
     7261 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg
     7321 catcttccac gacggtatat ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
     7381 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     7441 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg
     7501 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     7561 cgcgctccag tcgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt
     7621 gcggatgaac ggcgacgatt tcacgtcctt cctgatacgc cagcgcaata atgtcggtga
     7681 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     7741 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     7801 catctttgtg ttcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac
     7861 cggtcggatc atccagtttt accgtcagca gaccaatgcc accctgcggt ttagcatcac
     7921 gcagacgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     7981 cttcatcatc ggcaaagaaa ggtgcggcct gctgcatcgc atgacccgta cccagctgtt
     8041 ctgcctgaag cacccagttc aggttgtcgt ctttcagcgc ctgcttaagc agatcgccgc
     8101 cgtgaccata cacaaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     8161 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     8221 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     8281 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     8341 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc
     8401 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     8461 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     8521 ttttatatca tcaggatcta tgtgaacgct tttcaggacg ggtcaatacg cgtaaaaaaa
     8581 agccagcctg ttgccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt
     8641 ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc
     8701 gtaatctacg tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat
     8761 ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt
     8821 gccaggctgc acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg
     8881 tttcacgatg cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta
     8941 gatccccagt tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat
     9001 ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta
     9061 aggcgttaaa gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac
     9121 gcctgctccg gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg
     9181 tctttcaggg agacgtattt acccggagaa ccggtgaata cctctgccac gaagaacggc
     9241 tgggacagga agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac
     9301 agttcatcca tacccaggat ggcgataatg tctttcagtt cctgataacg ttgcaggatg
     9361 gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga
     9421 cggctggtgg agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg
     9481 ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcagg
     9541 tcatccgcag gtacgtatac cgcctgtacg gaggtgatag aaccggtttt ggtggaggtg
     9601 atacgttcct gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa
     9661 ggcatacggc ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg
     9721 tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg
     9781 gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat
     9841 actttgtcga taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta
     9901 cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaata
     9961 agctccatca tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg
    10021 cccttagcga acggacacat caggtcgata actttgatac cggtttccag cagttcctga
    10081 gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca
    10141 ccgatctcgc ctttcatgtc gaccggttca cccagtacgt tcatgatacg acccagagtc
    10201 gctttaccta ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc
    10261 agaccgtcgg aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga
    10321 acttccagca ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt
    10381 acggcatcct gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca
    10441 gtagccatct taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga
    10501 tctcggtgag ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct
    10561 ctttaatcag gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct
    10621 gctcgctggc caggttttca accacgccct gataaacctg agattcgaca taacgacgca
    10681 gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt
    10741 tcagatcatc atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag
    10801 acatggtgtt aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt
    10861 aggcctgcaa catcactttt accggaccga tcagttcgga caaggaaggg ttatccccca
    10921 tgccggtgac ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg
    10981 agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca
    11041 gcagtttttt gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca
    11101 ccaggtagcc cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat
    11161 taccgtgtgc aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg
    11221 ccatgcgatc ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga
    11281 tcttttgcgt gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga
    11341 gcttctcctc aatgccttgc ggcctgccca aaggcaagcc gccagacgtt accaggattg
    11401 ggttgctttg aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc
    11461 accggtctgg ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccaacag
    11521 agcggcttcg aagctgccga ttttcgacag ttcaacatcc gccaggtaac cacgttctgc
    11581 tgcgaacaga accagagact gttgcgcaac agacatcggc gcatactgtt tctgcttcag
    11641 cagttcggtc actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga
    11701 tgcaaactga gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc
    11761 ggacagtttt ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc
    11821 cgggttaacc gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc
    11881 atcggtaatg gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc
    11941 gataatcggc agcgcagtca gagaaccggt tttccctttc acttcacctt tggtgaaagc
    12001 ttcaacgtat tcggcgttaa cacgcgcagc acgctccagc agacgagagt ggaggtagaa
    12061 tacgtcgccc gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg
    12121 gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc
    12181 acggaagtat tcacccattg cgcaaccagc gtacggtgcc aggtattgca gtgcagcgga
    12241 ttcagacgcg gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt
    12301 acgtaccacg ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat
    12361 accggaatcg cgctggttga tgatggcatc gatagccagt gcggttttac cggtctgacg
    12421 gtcaccgatg atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt
    12481 ataaccggtc tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc
    12541 ttctacagca gagaagccgt cgtgatccag cggaccttta ccatcgattg gtgcacccag
    12601 agtgttaacc acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt
    12661 acacttaact ttcatgcctt cggcaaggtc agcgtacggc cccataacaa ccgcacctac
    12721 agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc
    12781 accctgcata caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat
    12841 agtaccttcg ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag
    12901 ttcgctgatt tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc
    12961 tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat
    13021 gataacacct gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag
    13081 acgtttttcc atcgcagcag aaattttcgc gagctgttgt tcactcaacg cggcagcgga
    13141 aatgacgtct acctcagcgg tagcctcact cacagcacgc aggtgaataa actgctccag
    13201 aacatccggg agcgcgttaa gacgaccatt ttcggccatt acccgaatca ggttctgacc
    13261 gttttcgtcc agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc
    13321 aagcgcgcca gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc
    13381 cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt
    13441 ggcgtagggg cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca
    13501 gcgacaagtt tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc
    13561 ttctcggcgc cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt
    13621 ttacgctcgg cttcaatttc cgcctgcgcc tgggccacga ttttagtacg ttcctgttct
    13681 gcctcagctt tcgcttcatc cagaatctgc gagcggcgtt tgttagcctg ctcgatgatt
    13741 acctgggctt ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca
    13801 aggtccttat gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt
    13861 tcgatggctg ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca
    13921 aacgcgatgg cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta
    13981 gttaacgttc tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat
    14041 cacgtacaga cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat
    14101 aaagaactga gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa
    14161 tttacccccg aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac
    14221 agcggcagcc atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt
    14281 tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga
    14341 cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg
    14401 cccacggcac attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga
    14461 aaatcagctc accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg
    14521 acagcaggct taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg
    14581 gctgcagcgt caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca
    14641 gaatcaggat aaatacgccc agtgccatag acagcgttac gttcacgtca gcagacggaa
    14701 ccacacgcag tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag
    14761 gcagtaaatc catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag
    14821 caatcagctt gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca
    14881 ccagctcaat cgcggtctga aacttacctg gcacgccgct ggttgccttt ttggctacac
    14941 tacggaataa aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat
    15001 tgattgtcca gaaggtggct ggggggttat gtggatccac cagcgagaat gtacgcaggt
    15061 ccagctgaag gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag
    15121 ccatgatgcc ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag
    15181 caccaaaacc cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac
    15241 caccagtaac accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc
    15301 cacccggcct ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac
    15361 gttaggcaga aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt
    15421 gaggctgaac agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg
    15481 agcaacgttt cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga
    15541 ggtatgccgc gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg
    15601 tcaaattata cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat
    15661 gtttaaatat ttttccagtg catacaattg cgacttttct gcaaaccctg ttcgatcatg
    15721 aaaaactgta aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg
    15781 catgttcgcg cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtt
//
LOCUS       DAPQZD010000111        15747 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPQZD010000111 DAPQZD010000000
VERSION     DAPQZD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.112"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            128..466
                     /gene="symE"
                     /locus_tag="RO454_003366"
     CDS             128..466
                     /gene="symE"
                     /locus_tag="RO454_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDY0935153.1"
                     /translation="MTDTHSIALPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAIEESELMQSLRQVCKLSVRKQRQVQEF
                     IGVISSKTPR"
     gene            complement(509..673)
                     /locus_tag="RO454_003367"
     CDS             complement(509..673)
                     /locus_tag="RO454_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDY0935154.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            850..2262
                     /locus_tag="RO454_003368"
     CDS             850..2262
                     /locus_tag="RO454_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDY0935155.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCGSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRTNCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(2505..3461)
                     /locus_tag="RO454_003369"
     CDS             complement(2505..3461)
                     /locus_tag="RO454_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDY0935156.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLEYASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPATARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELIRRMPQHRERIMTIAERIHNDGWLLGRERGRKEGKEEGEKSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPWIEY"
     gene            3928..5160
                     /gene="mdtM"
                     /locus_tag="RO454_003370"
     CDS             3928..5160
                     /gene="mdtM"
                     /locus_tag="RO454_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDY0935157.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATIALSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANTIVARFIK
                     DPTEPRFIWRAVPIQLAGLALLIVGNLLSPHVWQWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5201..6481
                     /locus_tag="RO454_003371"
     CDS             5201..6481
                     /locus_tag="RO454_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDY0935158.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARTDAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHHVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            6597..7748
                     /locus_tag="RO454_003372"
     CDS             6597..7748
                     /locus_tag="RO454_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDY0935159.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLHKTVEERFGHE
                     ISEDALRDAIVLKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            7758..8525
                     /locus_tag="RO454_003373"
     CDS             7758..8525
                     /locus_tag="RO454_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935160.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            8522..8779
                     /locus_tag="RO454_003374"
     CDS             8522..8779
                     /locus_tag="RO454_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDY0935161.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            8844..9704
                     /locus_tag="RO454_003375"
     CDS             8844..9704
                     /locus_tag="RO454_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDY0935162.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            9772..10950
                     /locus_tag="RO454_003376"
     CDS             9772..10950
                     /locus_tag="RO454_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935163.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSMLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(10963..11517)
                     /gene="kptA"
                     /locus_tag="RO454_003377"
     CDS             complement(10963..11517)
                     /gene="kptA"
                     /locus_tag="RO454_003377"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDY0935164.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKVGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            11767..12450
                     /locus_tag="RO454_003378"
     CDS             11767..12450
                     /locus_tag="RO454_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935165.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12447..12908
                     /locus_tag="RO454_003379"
     CDS             12447..12908
                     /locus_tag="RO454_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDY0935166.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12921..14093
                     /gene="iadA"
                     /locus_tag="RO454_003380"
     CDS             12921..14093
                     /gene="iadA"
                     /locus_tag="RO454_003380"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDY0935167.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            14158..15069
                     /gene="hypT"
                     /locus_tag="RO454_003381"
                     /gene_synonym="qseD"
     CDS             14158..15069
                     /gene="hypT"
                     /locus_tag="RO454_003381"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDY0935168.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(15062..15454)
                     /gene="iraD"
                     /locus_tag="RO454_003382"
     CDS             complement(15062..15454)
                     /gene="iraD"
                     /locus_tag="RO454_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDY0935169.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(15451..15534)
                     /gene="idlP"
                     /locus_tag="RO454_003383"
     CDS             complement(15451..15534)
                     /gene="idlP"
                     /locus_tag="RO454_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDY0935170.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3572 a         4039 c         4052 g         4084 t
ORIGIN      
        1 ttttctattt ttagctggac ttttcccata tttactgatg atatatccag gtatttagcg
       61 cgatgcggat gtgttggcgc acacccgcac cgctcatcac aatcactatt tatagggaat
      121 agcagttatg actgacacgc attctattgc actaccgttt gaagcagaag tctccccggc
      181 aaataaccgt caattaaccg tgagttatgc gagccgctac ccggattaca gccgtattcc
      241 cgccatcacc ctgaaaggtc agtggctgga aaccgccggt tttgccaccg gcacggcggt
      301 agatgtcaaa gtgatggaag gctgtattgt cctcaccgcc caaccacccg ccatcgaaga
      361 gagcgaactg atgcagtcac tgcgccaggt gtgcaaactg tcggtgcgta aacaaaggca
      421 ggtgcaggag tttattgggg tgattagtag caaaacgcca cgctaagttt ctctcccctt
      481 ccccacacgg gaaggggaat tttccccctc actccacatc ctccctgcgt aacccaatat
      541 ccctcaaccg ctcatcactc atctgctgta acactcgtcg ggtctgcatt tgccgccgcc
      601 aacgcctcac cgcctgccag agttgcacaa gtctgataaa cggcgttttg gctttgtttt
      661 cgtaaaattc catctgctgc ctcctcatct ttcagaggct tcatcatccg tcttctggca
      721 gttcacaata cagatgcaaa tattagtttt atttaacata cagacagatt aaaacagcat
      781 ctgcatccgg ttttttagcc tcatctgtac cggtttttct ctctgtatgg tccaaataag
      841 gaatacagca tgacgcgtta tcaacatctg gcgactctac ttgccgagcg gattgagcag
      901 gggctgtatc gtcacgggga gaaattgccg tcggtgcgca gcttaagtca ggagcacggc
      961 gtcagcatca gcaccgtgca gcaggcgtac cagacgctgg aaacgatgaa gctcatcact
     1021 ccgcagccgc gttcggggta ttttgtcgca caacgtaaag cccagccgcc agtgccgccg
     1081 atgacgcgtc cggtgcagcg cccggtggaa attacccagt gggatcaggt actggatatg
     1141 ctggaggcgc atagcgacag ttccattgtt ccgttaagca aaagcacgcc ggatgtcgaa
     1201 acgcccagcc tgaaaccgct gtggcgggag ctaagccggg tggtgcagca taatctgcaa
     1261 accgttctcg gttatgactt gttagccggt cagcgagtgt tgcgagagca gattgcccgc
     1321 ctgatgctcg acagcggctc ggtagtcacc gccgatgaca tcatcattac cagcggctgc
     1381 cataactcga tgtcgctggc gttaatggcg gtgtgtaaac cgggcgatat tgtcgcggtc
     1441 gaatccccct gttattacgg ttcgatgcag atgctgcgtg gcatgggcgt gaaagtgatt
     1501 gaaatcccaa ccgatccaga aacaggcatc agcgttgaag cgctggaact ggcgctggag
     1561 cagtggccga ttaaaggcat cattctggtg ccaaactgta ataatccgct gggatttatt
     1621 atgccggacg cgcgcaaacg ggccgttctc tctctcgctc agcgtcatga tattgtgatt
     1681 tttgaagatg atgtctacgg tgaactggca acggagtatc cgcgcccgcg gaccattcat
     1741 tcctgggata tcgacgggcg agtgctgctg tgcggctcgt tcagtaaaag tattgcacct
     1801 ggcctgcgcg tgggctgggt cgcaccgggg cgctatcacg ataaactgct gcatatgaaa
     1861 tatgccatca gcagctttaa tgtgccgtcc acgcaaatgg cggcggcaac gtttgtgctg
     1921 gaaggtcact atcatcgcca tatccggcgg atgcggcaga tctatcagcg caatctggca
     1981 ctttatacct gctggatacg ggaatatttt ccctgcgaaa tctgtattac gcgcccgaaa
     2041 ggcggatttt tactgtggat agaattgcct gaacaggtcg atatggtctg cgtcgcgcgg
     2101 cagctgtgcc gcatgaaaat ccaggtggcg gcaggctcga ttttctcggc ttccggcaaa
     2161 taccgtaatt gtctgcgcac caactgcgct ttgccgctca gcgaaaccta tcgcgaagca
     2221 ctgaagcaaa ttggcgaggc cgtgtatcgg gcaatggaat aaattcgaca tagtggcgaa
     2281 gcgcctgatt tactctcacg aatagagtgt gactcgtttt atctttgcag ggtaatcgcc
     2341 acgttccagc atggcttagg caaagtgcgc tttgttcata ccggatgcga tgtgaacgcc
     2401 ttaccaggtc tacaaaatct ttaaagttaa atatatttaa tgggttatgt agacctgata
     2461 agcgtagcgc atcaggctgc tttgcgtttg tcatcagtct ctgattagta ctcgatccat
     2521 ggatcgcgct tgctttcagg caagggctga tctaatgcct gcatttgctc tgccgaaagt
     2581 ccggtatatc gttgtatcca ttcaggatcc gccccattct gcaacaacaa tcggaggagg
     2641 ctcttttccc cttcttcttt cccttctttc ctccccctct cccttcccaa cagccatcca
     2701 tcattatgaa ttcgctctgc aatcgtcatt attcgctccc tgtgttgtgg cattcgacgg
     2761 ataagttcgc tgataaactc attaaaacgc gcttcatcgc cagtcagtaa aatgtaattt
     2821 aacagcgcgg ttatctggct gtcattagcg caccctgtaa ccagtaatac tgccagttga
     2881 tcaataagcc ccattagatc gcgctggcga atatgctttt ggatcaactc cagcagggcg
     2941 actctgcgat gctgcacaat ctcgtcgtct ggcacgacag tgacatccac cagcgggaac
     3001 gctgcggtat aaagcttccg tgcggtagcc gggtcggcaa attcatccag ccagcacaaa
     3061 gaccaggggt aaggactacg gctaccgtga taaaacagca tcgggatgac cagcggtagc
     3121 ggccggcgtt tatcatgctc gatatggcgc tgcatcaccg ccatggaata tcgcatcagg
     3181 cgaaaggcca tatggatatc ctcgcggctc tgatgttcaa tcaccacata aatatagcca
     3241 tctccttcgc gggtctttac cgaccacaga atatcggaat gtagcgcccg caatttttca
     3301 tcgacaaagc tggcgtattc cagttttaag ctgtcgagat cgcacagttc acgtaaatct
     3361 ttgggtaagt ggatctccat aaaatcccgc gcggtgtcag ggtgggtgag aaaagattta
     3421 aataatgcgt catgcggcgt gctggtcgtg aagtttgtca tggcgatccg tcacctgtag
     3481 ataagatggc ccgactgtag cgactaacaa aaccattctc acccggcaat ttctcgttcc
     3541 ccgaggtgct ttccaggcta tttttgttcc tctgcaaacg atacattttt ccggaaacag
     3601 gctcgcattt tacatttcct gtacccggca gatattcccc tttccaccgc actcttttga
     3661 ttaaccgcaa attttaatta cacttaaggt gtatattttc tatgcaaccc atcaattcaa
     3721 gaggtgtaat gtgctgatga ctatttgtaa tcgttataca ttctgacctg gagtcagaag
     3781 gtatttctct gtctgtatgt tcacaggcag tgtggttgat tacatgaaat tcagtacatt
     3841 tgcccgtctc gcctgccttt cgtcattacc gacggtattg aatttcgttt tccccattgg
     3901 gattctccgg acaaggagtt gtttgttatg ccacgttttt ttgcccgcca tgccgccacg
     3961 ctgtttttcc cgatggcgtt gattttgtat gactttgctg cgtatctgtc gacggacctg
     4021 atccagccag ggatcattaa tgtggtccgt gattttaatg ccgatgtcag tctggccccg
     4081 gctgccgtca gtctctatct cgctggaggc atggcattgc agtggctgct ggggccgctt
     4141 tccgacagga ttggccgcag gccggtgctg attaccgggg cgttaatttt tacccttgcc
     4201 tgcgccgcga caatgttcac cacgtcaatg acacaatttc ttatcgcgcg tgcaattcag
     4261 ggcaccagta tctgttttat tgccaccgtt ggttatgtca cggtgcagga ggcgttcgga
     4321 cagacaaaag ggatcaagtt gatggcgatt atcacctcca tcgtactgat tgcgccgatt
     4381 attggcccgc tttccggcgc agctctgatg cactttgtgc actggaaagt cctttttgcc
     4441 atcattgcgg ttatgggttt tatctcattt gtcggcttgc tgctggcgat gccagagacg
     4501 gtgaagcgcg gcgcggttcc gtttagcgcc aaaagcgtct tgcgcgattt tcgcaatgtc
     4561 ttttgcaacc ggctgttcct ctttggcgcg gcaaccatcg ccctcagcta tatcccaatg
     4621 atgagctggg tggctgtctc gccggtgatc ctgatcgatg cgggcggctt aacaacttcg
     4681 cagttcgcct ggacgcaggt tccggtattc ggcgcggtga ttgtcgccaa caccatcgtg
     4741 gcgcgattta tcaaagatcc gaccgaaccg cggtttatct ggcgtgccgt gcccattcaa
     4801 ctggccggcc tcgcactgtt gattgtcggc aatttgctgt cgccacacgt ctggcagtgg
     4861 tcggtgctcg gcaccagtct gtatgctttc gggattggtt taatcttccc gaccttattc
     4921 cgctttacgc tgttttccaa taacttaccg aaagggaccg tctccgcatc actgaatatg
     4981 gtgatcctga tggtgatgtc tgtctcggtc gaaatcggcc gctggctgtg gtttaacggc
     5041 ggtcgcttgc cgtttcatct gttagccgtc gtagcgggcg ttatcgtcgt tttcaccctg
     5101 gcgggattgc tcaatcgcgt gcgccagcat caggctgccg agctggcgga ggagcggtga
     5161 tttttgcgcg atcctgccgt caggctctat tcttaacgtt atgaataaac tcattgaact
     5221 cagacgcgcc aaaatgttgg cgctctcttt actgcttatc gccgctgcta cctttgtcgt
     5281 tacgctgttt ttgccaccca atttttgggt gagcggcgtg aaggcgattg ctgaagcggc
     5341 gatggtcggc gcgctggcgg actggtttgc ggtggtggcg ctgtttcgcc gcgtgccgat
     5401 tccgatcatt tctcgtcata cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa
     5461 tctcggtcag ttcgtgcagg aaaaatttct cgatacccaa tcgctggtgg cattgattcg
     5521 acgtcacgaa ccggcgttgt tgattggcaa ctggtttagt cagccagaaa acgcccgccg
     5581 cgttggtcag catctgttgc agatcatgag tggttttctt gaactgaccg atgatgcgcg
     5641 tattcagcgc ctgcttaagc gcgcagtcca tcgggcgatt gataaggtcg atctttccgg
     5701 caccagtgcg ttgatgctgg agagtatgac caaaaacgat cgtcatcagg tgctgctgga
     5761 tacgctgatc gcacagttga tcgcccttct ccagcgcgat aaatcgcgca agtttatcgc
     5821 ccagcagatt gttcgctggc tggagagtga gcatccactg aaagccaaaa ttttgcccac
     5881 tgaatggctg ggcgaacata gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga
     5941 tgatattagt cgcgatcgtg cgcatcagat ccgccatgcg tttgatcgcg ccacctttgc
     6001 cctgatcgac aagctgaaaa acgatcctga aatggcagcg cgaaccgatg ccgtaaaaag
     6061 ctatctgaaa gaagatgaag cttttaaccg ctatctcagt gaattgtggg gggatttacg
     6121 ggaatggctg aaagcggata tcaacagtga agattctcgt gtgaaagaac gcatcgcacg
     6181 agcgggtcaa tggtttggcg aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa
     6241 tggtcatctt gaacaagccg cgcaccacgt cgcgcctgag ttttccgcat tcctgacgcg
     6301 ccacatcagc gatacggtaa aaagctggga tgcgcgggat atgtcgcggc aaatagaatt
     6361 aaatatcggc aaagatctgc agtttattcg tgttaacggt acgctggttg gcggatgtat
     6421 tgggctgatt ttgtatttgc tgtcgcagct cccggctttg ttccccctcg gcaattttta
     6481 gaaatccatg aaaaactaag tggctaaccg cgagagagat caaataaacc gcactaaaag
     6541 aatggtaata atgtggcttc ttttcattat ctcccgtggt acggggaagg aaaatcatgt
     6601 cacttgtcac cgatctaccc gccattttcg atcagttctc tgaagctcgc cagaaaggct
     6661 ttctcaccgt catggatctc aaggagcgcg gcattccgct ggttggtacc tactgtactt
     6721 ttatgccgca agagatccca atggcagccg gtgcggttgt ggtttcactc tgttccacct
     6781 ctgatgaaac cattgaagaa gcggaaaaag acctgccgcg caatctctgc ccgctgatta
     6841 aaagcagcta cggcttcggc aaaaccgata aatgccccta cttctacttt tctgatctgg
     6901 tggtcggtga aaccacctgc gacggcaaaa agaaaatgta tgaatacatg gcggaattta
     6961 agcccgttca tgtgatgcaa ctgccaaaca gcgttaagga cgatgcctca cgcgcgttat
     7021 ggaaagccga gatgctgcgc ttgcataaaa cggtagaaga acgttttggg cacgagatta
     7081 gcgaagatgc tctacgcgat gccattgtgc tgaaaaaccg cgaacgtcgc gcactggcca
     7141 acttttatca tttggggcag ttaaatcctc cggcgcttag cggcagcgac attctgaaag
     7201 tagtctacgg cgcaaccttc cggttcgata aagaggcgtt gatcaatgaa ctggatgcaa
     7261 tgaccgcccg cgttcgtcag cagtgggaag aaggccagcg actggacccg cgtccgcgca
     7321 ttttaatcac cggctgccca attggcggcg cagcagagaa agtggtgcgc gcaattgaag
     7381 agaatggcgg ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg accgagcaat
     7441 gcgtggcaga aacgggcgat gtctacgacg cgctggcgga taaatatctg gcgattggct
     7501 gctcctgtgt ttcgccgaac gatcagcgcc tgcaaatgct cagccagatg gttgaagaat
     7561 atcaggtcga tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat
     7621 cgctggcgat taaacgtcat gtgcgtcatc agcacaacat tccttatatc gctattgaaa
     7681 cagactactc cacctcagat atcgggcagc tcagcacccg tgtcgcggcc tttattgaga
     7741 tgctgtaagg agtggcagtg gcatattcga ttggcattga ttccggctca accgccacca
     7801 aagggatctt actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc
     7861 gcccggcaac agcaattact gaagcctggg aaactctgcg cgaagggtta gaaacaacgc
     7921 cgtttctgac gctcaccggc tacggacggc aactggtgga ttttgccgat aaacaggtga
     7981 cggaaatctc ctgtcacggg ctgggcgcac ggtttcttgc gccagcaacg cgcgcagtaa
     8041 tcgacatcgg tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg
     8101 atttcctgat gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc
     8161 gcacgcttgg caccagcgtc gagcaactcg acagcattac cgaaaatgtc ataccgcacg
     8221 ccatcacgag tatgtgcaca gtgtttgctg aatcagaagt gatcagcctg cgctctgcgg
     8281 gcgtagcgcc agaagcgatt ctcgccggag tgattaacgc gatggcgcgg cggagcgcca
     8341 atttcattgc tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagccact
     8401 gtcaaaggtt tacccacatg ctggaaagcc atctgggaat gccggtacaa actcaccctg
     8461 atgcgcaatt cgctggtgca attggcgcgg cggtgatcgg tcagcggcag agaaaacgcg
     8521 catgaaagag tatctgtttt tatttcactc cacggtcggc gtcatacaaa cccgcaaggc
     8581 gttgcaggca gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg
     8641 ctgcgggtta tgcatttggc tgacctgtcc gcctggcgag gaaattcaat gggtgatccc
     8701 tgggcatacg gaatcagttt attgtcagca ggatggtggc tggcgctgca ttgcacatta
     8761 cgggatttct ccgcgttaag gattcattaa gatcgctacg tatacttctc gatatcttat
     8821 tgatttgcct taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatcttcg
     8881 ttattgtgat tctttttgtc attgtggcag tgtgtacatt cttcgtgcaa tcttgcgccc
     8941 gtaaaagcaa tcatgcagca tcatttcaga attaccacgc caccattgac ggcaaagaaa
     9001 ttgcaggcat aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt
     9061 ttagcactat caacaaacct gccgccattg ttgaaatgac caccaatggc gatttgatcc
     9121 gcacaattcc tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc
     9181 agtttgtcat tagcgatgag cgcgattatg ccatttatgt gatttcactg accccaaact
     9241 cggaagtaaa aatcctcaaa aaaataaaaa tcccgctgca ggaatcccca accaattgcg
     9301 gttttgaagg attggcctat tctcgccagg atcacacttt ctggtttttt aaagagaaaa
     9361 acccgattga ggtctacaaa gttaacgggc tgttaagtag caatgaattg cacatcagca
     9421 aagacaaggc actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc
     9481 aacaaaaaaa tacgcttctg gtgctgtctc atgaatctcg cgcactccag gaagtaacgc
     9541 tggctggcga ggttataggt gaaatgtcac taaccaaagg cagtcgaggt ttgtcgcata
     9601 acatcaaaca ggccgaaggg gttgcgatgg atgcctcagg taatatttat atcgttagcg
     9661 aaccaaatcg tttttatcgc tttacccctc aatcatcgca ttaacaggct ggcatgtttc
     9721 acctttttgt tattaacttg ttaacaacaa aaatgacaaa caaggttaaa catgccttcg
     9781 ccctcgcatc ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg
     9841 accttaggta tgggtttagg ccgcttcctt tatacgccta tgttgcccgt catgatggcg
     9901 gaaggatcgt tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat
     9961 ctggctggca gtctgctatt ttcgtttggc gcatttcacc agccatcgcg cctgcgccca
    10021 tttctgttgg cttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgctg
    10081 ccatttattc tggtgttatt gattcgcgtc ctggcgggtg tcgccagcgc cggtatgctg
    10141 atttttggtt cgatgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg
    10201 ttttctggcg ttggcattgg catcgcactg ggcaatgaat atgttctggc aggcctgcat
    10261 tttgacctct cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg
    10321 attgcactta cgcttttaat gccctcgaaa aaacacgcca tcacaccaat gccattggca
    10381 aaaacggagc aacagataat gagctggtgg ttactggcta ttctgtacgg cctggcggga
    10441 tttggttata tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca
    10501 ttgttaaccg cccatctctg gacgttagtc ggcttatcaa ttgtgcctgg ttgctttggc
    10561 tggctatggg cagcaaaacg ttggggggct ttgccctgcc tgacggcgaa tttgctggtg
    10621 caggctatct gtgtgctgct tactctcgcc agcgactcgc ctctcttgct tatcatcagc
    10681 agtcttggtt ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc
    10741 caactgagcg taccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt
    10801 ggacaaatcc tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc
    10861 ggcgccactc tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtacaa
    10921 ttatttaagc tgcaagtggt cacttcatga ggagtgtttc ccttaccact caagaaattc
    10981 gaccgcaacc gttgatgtca gccagacccc gttttccgct tgccagaaag ggaggcctcg
    11041 tttcgccatt tcctgtgctt tgacggttaa aataaccggc gatccgtgtc ttgctcccac
    11101 tttgcgtgcc gttgcttcat cagcagagag atgtacgtag tgacgctcac ctgcaatcag
    11161 cccctgtttt tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg
    11221 cggtgttttt tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg
    11281 gatgcaccta ccatcactgg aataactaaa acgcttttta tcgctggtgg caaccacggt
    11341 atccagcaat gcacgggtta accgcttgcc cactttttgg gcacaaagta tgagcttatc
    11401 gatatccgcc catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag
    11461 gacaaaactt aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc
    11521 gttttcgtcc ctggattatc tgttttttat ttactatgtc attgtcactg ggtttatgaa
    11581 ttttctgcaa ctcaatcctg cgtagaacct tttccacaca gcgtaatttt accgctatgc
    11641 aaaaaacgca tagtcagaaa catacttgca ttttccccat acccacatgg ctgataaggt
    11701 ttttcagctc caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg
    11761 attgttatga cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc
    11821 attaaagggt atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccggg
    11881 acagacagtt ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg
    11941 cctggtgcga acggagccac cacctcgttt cgcggcgcgg gtggcgcggg ggctaaagat
    12001 ggctttctct ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca
    12061 attaccgatg gtcttggcgg attacgcgcc gcgcagcagt taatgacgcc agtgctaaaa
    12121 ccgttgttgg gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacacc
    12181 gatgccgccg caggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat
    12241 aaagtgattt tcgccgcata tcaaaccagc ggtagcgcca ttatcaccaa ctacttctct
    12301 tccggcgtcg cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agcagtcatc
    12361 ctggtgttta aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt
    12421 cgcaacccaa cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata
    12481 tgtttatcga tggtgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg
    12541 tggtgatggc gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg
    12601 ggcatatctg tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc
    12661 ttttagccgc attaatgagt atgggtggcg cagtcggtgt tgccgccagt ctggcaaccg
    12721 ctggcgcatt aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca
    12781 acccggtgca aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc
    12841 cgcatatcat tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta
    12901 ttgtttaaca aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg
    12961 gagcgcattt gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca
    13021 aaattatcgc cgttgccagt aatatccctt ctgacattgt accggactgc acggttgtcg
    13081 atctcagcgg gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg
    13141 gtggcggcga agcaggtccc acgacgcgca cgccggaagt ggcgttaagt cgtctgacgg
    13201 aagcaggcgt cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg
    13261 aatccctgct cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga
    13321 ccggcgctta tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcga
    13381 ttatcgatcg cgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg
    13441 acgtttatca cctggctaat atggcggcgg aatcccgcgt tggcggtttg ctcggcggta
    13501 aacctggcgt caccgtgttc cacatgggcg acagcaaaaa ggcgttacag cctgtctatg
    13561 acctgctgga aaactgcgat gtgccgatta gcaagttgct gccgacccac gttaaccgca
    13621 acgttccgtt gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca
    13681 ccagcagcat tgacgaacca gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg
    13741 gtattccgct ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg
    13801 atgacgaagg gaatttaacc catatcggtg ttgccggttt tgaaacgttg ctggaaaccg
    13861 tgcaggtgct ggtcaaagat tatgatttca gtatcagcga tgccctgcgc ccgctcacca
    13921 gtagcgtagc cggtttcctt aacctgagcg ggaaaggcga aattctgcca ggcaatgacg
    13981 cagacttact ggtcatgacg ccagaattac gcattgagca ggtatacgct cgcggcaagc
    14041 tgatggtcaa agacggcaaa gcctgcgtga aaggaacgtt tgaaacggca taaacggttg
    14101 tcagccgccc gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg
    14161 gatgactgtg gtgcggtttt gcataatatt gaaactaaat ggctttatga ttttctgacc
    14221 ctggaaaaat gccgcaattt ttcccaggca gcagtcagtc gcaacgtctc gcaaccggca
    14281 ttcagccgcc gcatccgtgc gctggaacag gcgattggcg ttgaattgtt taaccgccag
    14341 gtgacgccgc tgcaactctc ggaacaaggt aaaatattcc attcgcagat ccgccatctg
    14401 ttgcaacagt tagagagcaa ccttgcagaa ctccgtggtg gcagcgatta cgcgcaacgc
    14461 aaaattaaaa tagccgctgc acactctctt tccctcgggc tgttaccgtc cattatcagc
    14521 cagatgccgc cgctctttac ctgggcaatt gaagctattg atgtcgatga agcggtcgat
    14581 aaactgcgtg aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa
    14641 gcgccgtttg accacattcg cttatttgaa tctcaattgt tccccgtttg cgccagtgac
    14701 gaacacggag aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc
    14761 cgcaactcct acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt
    14821 ttcagcacct tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc
    14881 tgtgggattg cctggctacc ggaatacgcc atccaacaag aaattcgcag cgggcagctc
    14941 gttgtactga atcgggacga actggtgatc ccgattcagg cctatgcata ccggatgaat
    15001 acccgcatga atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg
    15061 cttagctgac attctccagc gtcgcactgc gccaaccgcc attatcccag accagatcaa
    15121 acgttagcga ttcatcgtcg tgatacagca ttatctcaag gctgatcgcc agagtgtggc
    15181 agtacgcttc tttcaaatta acctgtaacg ccgcaatccg tggctcatga aataacagtg
    15241 cagaacgcag ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt
    15301 gccactcggg catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa
    15361 gagtgaggag atcgctgcat actgatttac gcagcggtga cgttttattg cctgaaattt
    15421 caggtaaaac agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac
    15481 atatgcggct tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat
    15541 tcccttttgt tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt
    15601 ataaaacctt acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga
    15661 taaaaatcat caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttac
    15721 gtagatgaaa atacttgtca ccaccgc
//
LOCUS       DAPQZD010000112        15742 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPQZD010000112 DAPQZD010000000
VERSION     DAPQZD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.113"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            95..739
                     /locus_tag="RO454_003384"
     CDS             95..739
                     /locus_tag="RO454_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935171.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAVRKKPTDAL"
     gene            994..2079
                     /locus_tag="RO454_003385"
     CDS             994..2079
                     /locus_tag="RO454_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDY0935172.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDYVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            2309..4225
                     /locus_tag="RO454_003386"
     CDS             2309..4225
                     /locus_tag="RO454_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0935173.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMSRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            4215..4958
                     /locus_tag="RO454_003387"
     CDS             4215..4958
                     /locus_tag="RO454_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935174.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            4968..5537
                     /locus_tag="RO454_003388"
     CDS             4968..5537
                     /locus_tag="RO454_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935175.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQSSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            5611..7926
                     /gene="nrdA"
                     /locus_tag="RO454_003389"
     CDS             5611..7926
                     /gene="nrdA"
                     /locus_tag="RO454_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDY0935176.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPETPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            8032..9174
                     /gene="nrdB"
                     /locus_tag="RO454_003390"
     CDS             8032..9174
                     /gene="nrdB"
                     /locus_tag="RO454_003390"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDY0935177.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            9252..10121
                     /locus_tag="RO454_003391"
     CDS             9252..10121
                     /locus_tag="RO454_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HDY0935178.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASYMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADAEERRHAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMFKK"
     gene            10293..11396
                     /locus_tag="RO454_003392"
     CDS             10293..11396
                     /locus_tag="RO454_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDY0935179.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIDTFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEVLILGYDPLPWIKVPVAV"
     gene            11410..11811
                     /locus_tag="RO454_003393"
     CDS             11410..11811
                     /locus_tag="RO454_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935180.1"
                     /translation="MYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSRMLRG
                     FARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLAEHKG
                     DLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            11808..12284
                     /locus_tag="RO454_003394"
     CDS             11808..12284
                     /locus_tag="RO454_003394"
                     /inference="COORDINATES: protein motif:HMM:NF012413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDY0935181.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYLAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            12284..12928
                     /locus_tag="RO454_003395"
     CDS             12284..12928
                     /locus_tag="RO454_003395"
                     /inference="COORDINATES: protein motif:HMM:NF024911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0935182.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMSLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            12990..13409
                     /locus_tag="RO454_003396"
     CDS             12990..13409
                     /locus_tag="RO454_003396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935183.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGTQVGEAINIAMSQFSEGSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TVSGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            13419..13976
                     /locus_tag="RO454_003397"
     CDS             13419..13976
                     /locus_tag="RO454_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDY0935184.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            14137..14946
                     /locus_tag="RO454_003398"
     CDS             14137..14946
                     /locus_tag="RO454_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935185.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVIKAESVESAENEPEQL
                     GFIEVEECVEVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDLFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            15129..15722
                     /locus_tag="RO454_003399"
     CDS             15129..15722
                     /locus_tag="RO454_003399"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDY0935186.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
BASE COUNT         4358 a         3390 c         3861 g         4133 t
ORIGIN      
        1 gtggatatgt gaataattaa aacaggcatt tactaccttc ggcgcacctg gtgggttatc
       61 gttgcctcgg ctaacaatca cagaaaaatg acatatggat ctaaaacgca cgcgctgggt
      121 tcgtcgtctt gaagacggct cctacactat cgaatcaaat tccaacctga ataagcagaa
      181 gttgctttgt gacatctgcg gtatagcggc gaagtgcccg atctacgaaa ccagaattaa
      241 acttgataag gctggtgtga attttcattt aaacagttgc atcaggtacg ttccattact
      301 cgcatttcgt aaaccgatca tcggattgga tgctccctac ttcaacacac tccgttcagg
      361 tgtgacgtgg cgagatcgtt tatcaccaga caagctgatt tgcctcgtat ccgcagacac
      421 agggaaaatc atccgttttg ggaaagtaga caaggtttac tcaggcccag tagacgaaat
      481 gttgcggaaa cacagccggt ttaatcatct ctgtatgggt ggtgagaaaa tcgagaaggt
      541 agaagaagtg atccgcaaat cctacggaca ctttctgacc aaagatagcc tgctcaccgc
      601 aatctacatc agacatgtaa aacgtgagtt cgacctcgaa taccacagcg aagaagagct
      661 taaccttgtt gacccacgtc caaaagctgg cgtcataagc ataaacgcag tgcgtaaaaa
      721 gcccactgac gcgctgtaac cctccagatc gtatattggc gtagatagaa tctacgcctc
      781 ctcaaaatag ctctcatagc gttctacagt gatcctgtct tatttttagt catacagaca
      841 agcaaagttg cgccacaata aataggtata tacttactta taaattttgt atattaaggc
      901 gctcgtttca ttcctaacat accgttatgc atagttgttt accttctcat tgctcttaaa
      961 atttgtatca aaataaccac aaaggaaaaa cacatgactt tgccatacgg cgtcatttct
     1021 gactgccact accacaaatg ggatgcgttc tccacgacga acgcagaggg gcttaactcc
     1081 agacttgaaa tacagttgga agcaacgaaa gaagcagcca tcgccatgaa gaaggccggt
     1141 tgtaagtaca tgttggttgc cggtgataca tttcacgtcc gaggaactgt gtccccttct
     1201 gttttgcatt acgtaactga aacgtacaag tggattatca acgagcttga tctgacagta
     1261 gtaatgctgg ccggtaatca cgatcttgaa accaacgatt cagtatatag cgccaacgca
     1321 gcagcatcgc tgagttctat cggcgtggta atcgtatgtg gcaaacgccc acactcaata
     1381 aaaattggtg atgtgactgt ccacctgatt agctggcgta acaatcatgc ggagcttatc
     1441 agcgatctga aagcattacg taagagcgta gaaggtgata atcatgacgt tgttatccat
     1501 acatccatta acaaagccat tccaacaatg cctgacgtcg gtatcgatgc gcaggagtta
     1561 aaggatatcg gctttcgtct cgtgcttagt gggcattacc acaaccacaa agaggtcatt
     1621 cctggagtta tcagtgtcgg tgcgctgacc catcaaaatt ggggagatgt tggatctctg
     1681 gctggttaca tgatcgtaaa cccggacggc agtttcagtc actacgaaac cagtgcgcct
     1741 aaattcatta acctggaaga ttatgttgcc gatgaccaaa ttcgcggcaa ctacgtgcgt
     1801 ttccgcgccg taattgagaa cgatgaagaa ggcattaagt accagaacat cctcaaaaca
     1861 atgggtgcaa aaggtgtcgt gtgcaacttc atccgtaagt catcaatgat ggaagggaca
     1921 gcaagcacaa cggaaaccag caaaatcgat agcctgggag agtcggtatc tgcttattgc
     1981 aagattgtcc acgatactga cggcggattt gatctgagca agttggatat tttgtgtcag
     2041 gaaatcctca ccgaagcgga gagttcggag gctgtgtgag gcaaagtcgt tatgggagct
     2101 ttcgagactt tgccatcacg atgaaaagac ttgaacgagg ccagacggtg atgtttcaca
     2161 agccctaccc gccacaagga aatcccgtag cgttttatct tggaaggtta accagaaaag
     2221 gcgtattgag gcgcagatcc ttcccggcgc atacggagtt cagattgaaa gaaggtcaaa
     2281 agctaacaca cggtatcaaa ggtgttatat gaagttttta aagctccagg ttgagaattt
     2341 tatggctatc gccagcgcgg aggtcgagtt agatcagcgt ggtttagtgc tcattcaggg
     2401 tgttaatagt gatgatagtt ccgcatcaag taatggctct ggaaagtcaa cgctaatgaa
     2461 tagcctgatg tggtgtcttt atggcgaaac agctcatggt gtgaagggtg acgatgtgtt
     2521 gtctaccgac catgaaaaga actgtcgtgt tgcagtaacc atcgaggatg aaggcaagag
     2581 atatgcgatc attcgtcacc gtaaacacaa agagttcaaa aatcggctta tcgttcgtgg
     2641 tgaagacggc gatatgacga aaggcaaaga tgcgctgacg caagagttcg tcgagcgtct
     2701 gatcggtgca tctaaagagg ttttcatggc ttccatctat gcgagccaag aagctatgcc
     2761 agatttacct ggaatgtccg acaaaaacct caaaaccatc gtagaagaag ccgctggcgt
     2821 tgacagactg acacgcgcct acgctattgc tcgtgagcga gctaatgcag ctgccgcacg
     2881 tatggatgtg gttaaaacca aattggagtc gacaatctcg accattgagg caacacagtc
     2941 agaaattgag tcagcgaaag cctcctctga atcatgggag caagagcgtt ctaaacgtta
     3001 tgacgatgcc ctggctgggc tggccagtgc cgaagttgag ttaacggaag ttgaacttga
     3061 gatccgcact cttcccgaac agatccgtga taccgagaag gcaatcgaaa gtgagcgcaa
     3121 aaagttagcc tcaaaagaag aacatgacgc caagttgctc aaagttcgtg gtgcgataac
     3181 tgatattcgg gcaagcatca aagctacaga aaatagtcag gctgatgcaa tgagccgcgc
     3241 gcgtaatttt aagaccaaag cagaagaggt tggtactaaa gtgggatcac catgtcctac
     3301 ttgtggcaaa gcctactgcg aagaagatct atcaacggtg aaggagaatt tcattgaaca
     3361 agcacgtcag gaaattggtc aggcgaagac acttgcagag gcaatggcta aacacaaaac
     3421 gaatcttgag aaagcgttaa gcattgagtc tgcccttgtt aaaacgacac ctgatgtaac
     3481 ggctatcatt gcccggattg aagagcttac gaaacaactc tcatctttgc gtcatcgtga
     3541 gaaggaggtt gttgctattg agtctcttgt gactcgtgct cgtactgagg tcgatcgtat
     3601 atcaaaagag attaatccgt ttattgctct tatcgccaga cacgaagata acctggtatc
     3661 cagtaagtct accttcaagt ccttaaaaga tgagttgaag gctattcagg aacaaacgtt
     3721 gctattggaa aaagctcgtc aggtctactc tcctgccggg gtgcgttctc atattttgac
     3781 gtctgttacg cctttcctga atacacgcac tgccgagtat ctcaatacgt tgtctgacgg
     3841 gaatattact gctgagtggt cgacgatgga tgtcactaaa aaaggtgagt atcgcgacaa
     3901 attcaacatt agtgtgcaga agaaaggttc aagtaagtcg ttccagaccc tctctggtgg
     3961 tgagaagcgg aaggttcgca ttgcgtgttc tttggcattg caggatctgg ttagtaaccg
     4021 ggcgagtaaa aacatcgatt tgtttatcgg cgacgaaatt gacgatgcac tcgatacagc
     4081 cggtcttgaa cgcctcatgg gtattctgga gtccaaagct cgcgagcgag gtactgtgct
     4141 gattatctcc cataaagaga tgaagtcatg gttccgggaa actattacgc tggaagttaa
     4201 agaggggcgc agctatgtcg tttaaattaa gccgctcgca gtttttgcag gtatttgcag
     4261 tgatgcagtc gataaaactg atcaatgggc atacttccaa tggtgcggct ccacgtattc
     4321 tgtggggcag caacaatatt gacggagtac aattcgccgc gttgcttggt ctaatatccg
     4381 agacgccatt gatgcaaagt ttgaaatcac taccacctgg atgtattgcg ccgatcctga
     4441 ttaatccttt tgttgagggg ggatatcttc ccaacgtcgg gcctgggttt attgcatccc
     4501 atgaaactga agatcttaac attaatagcg aagggttctt tgggggaatg ggtgcgcatc
     4561 actgtatggc tttcacgaac cttattcgac ttgccaataa gcgggtggat agtttggcat
     4621 cgccaggtga tgcttttact ggtttcctta tccaaaggag ggataaaaag tacagtgcgg
     4681 acaaactaca gtttgttggt aagtatggag aaatggtaga aatcgaactt cagctccctc
     4741 atgttttagc aaacgatagt gcagacagtc ggaggctgtt gggcatcatg cgtcatttca
     4801 tagcaagtgg cgttaaacat gccgtagata aacgtgtcac gcaggaaaat gagtattcag
     4861 actttgcaaa ctctccccaa ccaacgttgc aaacggcaat agtaaccaat tcgttggagg
     4921 cgagattatt ggaaaaccct atatggggaa catggtaagg agactatatg agtaaaaaaa
     4981 tcagcgtagt tggtgttgat ccctcaatga gcaactttgg gcttgctgtg ggcactttag
     5041 accttgagac ggacgaactt gagattcacg gccttactct tgttgagact aaggcgggga
     5101 gtaacaaaaa gaccgttcgt gtgaacagtg acgatctgcg tcgtgccagt gaaatatggc
     5161 gtgttgcgaa gccaatcatt gataaggcaa atatggtttt ttgtgagcta ccggttggga
     5221 gccaaagctc tcgttcgcag acgtcttacg gtatttgtat cggtgtactt gcgtgtgtgg
     5281 ataagccatt gatccaggtt actccaaacg aaattaagca ttttgtcggc aataaactta
     5341 ctacatcgaa agaagagatt atccagtggg ctacgaaaaa acaccctaaa gcaccgtggc
     5401 tgcgtcgtaa gcaatctgga caggatgttc tcgtgaacaa aaacgaacat ttggctgatg
     5461 cggtggctgc catccatacc ggtatgcaaa cagatcagtt ccgccaggtg cgcgatgttc
     5521 ttaagtctct catttgattt cattgatagg taagtactta tctattaaca tgggccacta
     5581 tatttagtgg ccctctttat ttggtgatac atgataagca tcgtaaaacg taacggccaa
     5641 acagagccgt tatccgaaga aaaatacaac cgcgtcgtaa tgtatggcgt agaaggcatt
     5701 cgtggtgtaa gcgcatccgc tgtagcaatg ggagctgcgg ccagcatttt tgatgggatg
     5761 accaccagcc agttgcatga ggctttggtt aaatctgccg ctgatttgat ctcaccagaa
     5821 acaccaaatt actcacaggt ggctgcccgc ctgaacattt ttaaaatccg caaagatgcc
     5881 ttcggtcgtt acgactatcc gaacttctac caacacattg tcaagaacgt taacaagggc
     5941 gtttatgaca aggatttgct gacacattat tcgtttgaag agatcgaaga actcggcaat
     6001 tacattaagc cgaaacgtga cgatcttttt ggctatgcag ctacggtgca gttgcaaagc
     6061 aaatacctcg ttcaaaaccg tgttactggt gagattcatg aggggccgca acatatctat
     6121 atgctggtag gcatgtgtct gttccagaat tgggaagacg actgcgctgg caaaacacgt
     6181 atggagatgg tcaaaggttt ctatgacgtt acaagtacgt tcaaactgtc tctgcccaca
     6241 ccaatcatgg ccggcgtccg tactccaacc cgtcagttct ccagttgtgt gctgattgag
     6301 tctggcgata gtctgaaagg gattagtgca gcttcagccg caattatcga ctacgtttca
     6361 cgtcgtgctg gaattggtat tggttttggc cgtatccgtg cgctgggcag cgagatccgc
     6421 aatggtgaag ccacccatac cggagttatt ccattcctga agcatttcca gacggctgtt
     6481 aaatcttgtt cgcaaggtgg tgttcgtggt ggcgcagcaa cagcgtttta cccgatctgg
     6541 catcttgaag ttgaaagtct gctggtggtg aaaaataacc gtggtattga tgaaaaccgc
     6601 gttcgccatc ttgattacgg cgtcatgagt aaccgtctaa tgtaccgtcg actcgtcaga
     6661 agcgagaaca tcactctgtt cagcccgcat gatgtgcctg atatgtacga agccttcttc
     6721 acagaccagg agctgtttga aaagctgtac cataaatacg aagccgatga ttcaattcgc
     6781 aagaagtcag tacctgccat tgagctgttc tcatctctga tgcaggaacg agcgtccacg
     6841 ggccgaattt atattgcgaa cgtagatcat attaacgagc atggcgcttt cattcctgct
     6901 cttgcacctg ttcgccagtc aaacctgtgc atggagatca ctctacccac tcgtccactg
     6961 gcatttaccg acgacccgaa cggtgagatc gcgctatgca ctttatccgc ttttaacctc
     7021 ggagccatcc gttcactgga gtctcttaaa gaggtggcgt tctatgccgt tgctgcactg
     7081 gattcgttac tggattatca agactatccg atggaggcag ccgaagtgcc tgccaaagct
     7141 cgtcgtagct tgggaattgg tgtaaccaac tttgcttatt acctggcaaa gaatggcgtt
     7201 cgttattctg ataccgctgg caataaactg gtgcatgaaa cgttcgaagc tatccagtat
     7261 taccttcttg atgccagctg ccgacttgct gaagcaaaag gtgagtgtga ctggtttgag
     7321 cagaccaagt acgcaattgg tcagttgccg atcgaccatt accgttcttc attagacgaa
     7381 agtggcgaaa ccaactttga gttaaagatg ccgtgggaag aactgcgtga acgtattgca
     7441 aaatacggcc ttcgcaactc cacactgacg gcacaaatgc catgcgagac ttccagccag
     7501 atcactaatt ccaccaacgg catcgaaccg cctcgtggcc cggtgtcggt gaaatcttct
     7561 aaggacggca tcgttaagat ggtcgtgcct gagtttgaaa aactgaagga acagtatgaa
     7621 tacctgtggg atatgccgga caaccgcggc tatctgacaa aggtggcgat catccagaag
     7681 ttctttgacc aggctatttc agccaatacc aactatgacc cttctcgctt tgaaggcgat
     7741 aaagtcccaa tgatgacgct actgtcagat ttgcttctcg cctacaagat gggagttaaa
     7801 acgctttact accacaacac cagagatggg gcaggaaagc gtgatgacga cgaaccgcag
     7861 aatccactga cgcaagctgt agccgtcgag ccagaagatg agtgcgacgg agcctgcaaa
     7921 atctgacata tggtggggtg tatccccacc ttctctttga tttgtaagcc ttgtttaaac
     7981 aaataagata acaacttgtt taaacacatc aaaaagcaaa aggaaaaaca catgtcatat
     8041 tcaacgttcc gtttgggtgc taatgatgca accaaagagc ctatgttcct cggacaatct
     8101 gtcaacgtgg cacgttacga tcagcaaaaa taccgtgatt ttgaaaagtt gattgaacgt
     8161 caattgtctt tcttctggcg gccggaagaa gttgatattt cgagcgatcg tatcgacttc
     8221 aacacgaagc tgcgggacca cgaacgtcac atttttctga gcaatctccg ttatcaaacg
     8281 ttactcgatt cagttcaggg acgtagccca aatgcaacgc tgctgccgct tatctctatt
     8341 cctgaactgg aaacgtgggt tgaaacatgg tctttctctg agactatcca tagccgcagc
     8401 tacacccaca ttattcgtgg catggtggac gatccgagca ttgtttttga cggtattgtt
     8461 acggatgaag aaatcatcaa ccgagcgatc agtatctctg ctgaatatga caggctttat
     8521 gggatgacct gcgagcgcca gtcgttaggt gagaaagagt ttgaacgtct gtacgtaaat
     8581 gaatatggct gggagccata ccctttgcac cgtcagcttt tccgcacgtt ggtgtccatt
     8641 aatgcgcttg aggcgatccg tttctatgta agttttgcat gtacgtttgc ctttggcgaa
     8701 cggaagttgc ttgagggtaa caccaaaatt atgcgcttta ttgcccgtga tgaagctctg
     8761 cattgcgaag gaactgaacg catgatccgc ttcatgcgta ccggtcgcga aggtttattg
     8821 tggaaagaga ttgctgctga tgaagaaaac gtcatttacg acaccatgaa atcagtcgcc
     8881 gaacaagaaa tgaactgggc agactatctc ttcaaagacg gttcgatgat tggtttaaac
     8941 gcggatattc tgaagaccta tgtaaaatac cgaaccaatc tggctatgaa tcgtcttggc
     9001 ctgaaggctt tatttccaga agttaccaca gatccgctgg tctggatgaa caagtggttg
     9061 ttaaccgaca cactgcaaat tgcaccacaa gaggcagagc aaagcacata tctggtaggt
     9121 cagatcgatt ctaccgtgga caaggcttct ctaagccagt ttgcagacct gtaaaccgat
     9181 acaaagcatt atgtggcctg gcaacgctgg gccacaatgg atcacaagaa ttaagaagga
     9241 acaaaactag catgaacttt accaaactga ctgaccacct gaaacttgcc accgatcgtc
     9301 tcattggatt taagccagaa ccatatgagt tgcatgaagg tcatggtgta gctactgaaa
     9361 gtatttacaa gatggtcgat cagtttcatg aactcttcca gcatccgaga cgcgttatgc
     9421 cgacaccaga gctgcttcgt ctccgtgcaa gcctgattca tgaagaagct gtagtggaag
     9481 gtattccagc tgcaatgaat ggggatattg agcaactgct ggatgcaatg gccgactttt
     9541 tatacgttgg tgttggtacg atggtcgcca tcaaaggtgg tatttctacc ggcatgacct
     9601 attacacgca ggaacagagc attgatcgct ttatgcagac aatttttgtg cctggtaaca
     9661 ctgttttcga tgatatggca atgccatttc aggaagctcg tgaggcgtca tatatgctcg
     9721 aagagctggc agataaactt gagaacaaga ctgttaagga ttctgagctg attcaggaac
     9781 tgcgccgtgt aatgaacaaa atctatgtgg cgtgcatgat gacctatcga ctggctgatt
     9841 tcctcggtat caatgtcgtc gagctggttg gcgaaattca tcggtccaac atgacaaaat
     9901 tatggcctgc agatgccgaa gagcgtcgcc atgctgtggc caactgcaaa tacgactctt
     9961 ctgacctggg atttcgccat gctgatggca ccgataagat gatcggtttt cgaatttccg
    10021 atggaaagat tctgaagtct ccaacctata gtgatgtcga tttatcctcc tttgttgagc
    10081 aagctaaagc ctcagcaatg tacggaatgt tcaaaaaata attgtaggta gttatctatc
    10141 tgtgtatatt gcattggcgc gttaaatttc tgaaacaact attcgttttt ggtggcctat
    10201 ggccaccatt tttttatctg tctggtcttg ttttctcaat aaatgtaaac tcacgcaatg
    10261 aataagtggt tacttatctt tgtgaggttt ttgtgtcact ccttttgaat cgtgagcata
    10321 cgaacggtca ggtaacaaac gcatcgtatg caaaagttat tgagacggtg cttaaaagcg
    10381 gcgtgcaggc tgatgatcgc acaggcactg gtactttaag cacctgctac gttccctctt
    10441 actacatgct tactggtggg actgtgccgc ttatttctgg aaaggcggta aatcttaagc
    10501 cactacttgt cgaacttgag tggtatctga aaggcacggg caacatccaa tttctcaagg
    10561 ataacggcgt taagatttgg gatgcatggg ccgatgagaa tggcgatttg gggccggttt
    10621 acggtaagca gtggcgtcga tgggaagata cccgcatcgt gagccatagt gaatatctga
    10681 gcaagatcga tactttccgt gaacgcgggt acaaagtcga gggatacctg ggtatcagtg
    10741 aagatcgcgt agtgctgtcc cgtgaaatcg atcagctaca gcgtattgtc gatacactgc
    10801 gcacgaaccc taccgatcgt cgcatcatgc ttaacgcatg gaacgtaggc gagcttgagg
    10861 atatgaaact gccaccttgc cactttgtct tctctttgtg gagtcgtgag ctggattttg
    10921 aaacccgttt aacgatggca actgacattg gtcttcaaca cagtcgcctc ggttacgagt
    10981 ctatctacac caagatgcta tacgatctgg agatggacgg cagtgttact gaagctgaac
    11041 tggatgaact tggaatcccc aaacgcatcc tcaactcctg cctcgtacag cgtagcgtag
    11101 acacttttgt tggtatgcca ttcaatattg ctggctatgg cattctcact cattttctcg
    11161 cgaagattac gggtcacatg gccggtgcat ttgtgcattt tggctttgac gtgcatttgt
    11221 acaacaacca catggaaggt gtgtgtgagc taatgaagcg acaggctcca gagcattcag
    11281 atccggtcgt tattttccct catgaatggt cagagttgga tgatttcaaa tgggacgagg
    11341 ttttaattct tggctatgac cctctaccgt ggatcaaggt tccagtggcg gtgtgatatg
    11401 gcaagaggta tgtatgtctt atgcgaaatt gaaggtgtgc tggcaaatgc cagccatcgt
    11461 aaatcagtat ctgacgcgga tgcaggccag ctcattgccg gtgatgaact cattttcccc
    11521 accagccgta tgttgcgtgg ttttgctcgc tcaggggctg aagtggtgct tatcagtagc
    11581 cgctctgaaa ctcttgaagc gccaactaaa cgatggctga aagattttgg cgttgattat
    11641 gactggcttc atctcgtacc gaatggcacc agttatgaga agcatattaa gcgcacatta
    11701 gcggagcata aaggcgatct gcttatcgct gcgctggtgc acgatcctcg actccgtgcc
    11761 gctttagccg actctcacca tcgaccggtc atctatgagg tgagcaaatg aagatgatcg
    11821 ctgctgttgg ccgtaactat gagatcggca tagcgaatga actcccctgg cgttgttcta
    11881 ccgatctgaa gctatttaag agactcacca aaaacgccac tgtcgttatg ggacgtaaaa
    11941 cgatggaaag tctcaaacgc cctcttccag agcgtcataa cctcgttttg acgcgctctc
    12001 atggctttgt accaaatgga ttctaccttg ctggtgtgga tgatgtgttg cgattaccag
    12061 agcctgtgtg ggtgattggc ggggaacaaa tttactcgct attcatgccg catgttgaag
    12121 agatttggct ctcccacatc ggcgttgatg taccaaacgc cgatgcattc ttcccggcaa
    12181 gcatgatgcg taacttaggc tttgtgcctg ttgaaacagc ttatacccaa cgagccagcg
    12241 aggaagagcc tggcttttcg cagatcgtat acagaaggtc gtaatggatt accggattgg
    12301 gatcactggt gctcagggca gtgggaaaac aaccctggct aaatatatcg acaaacatta
    12361 cggaatccct tacgtggatg ctggtgtcgg aagtttgatg agccgcctcg gtgttcgagt
    12421 aggtgagtct atgtctctat atgagcggct tcagattcaa atggaaatag caaagcatat
    12481 agagctactt acgcgtggtg ttgaaggctt tgttatcgat cgcacacctg ctgatgttat
    12541 ggcctacacg ttggatttgg tcggccatac aaatgaagat cggtgtattg agttagccct
    12601 cgatatcgaa aagttttgcc acaaaactgc tatttcaaac tttaacgcca ttgctggctt
    12661 acgcccggga gtcgctctct cagagcgaga ttacttgcga tcacaacgag catcattaga
    12721 ccgtctgtat gtcgctcgta ttgatgcgtt gatgtgcggg gaactgacaa aaattcacct
    12781 gcatccgcaa aggggagatc tgcaaacctt cgtcgtttcc aaccgatatc gcacggttga
    12841 agcaagagcc agatcagtga tgagaatgct agataacgct gtagaaaaga tagaaaaccg
    12901 gttctgtggc cgagtgaccg ttcattagaa attgttcgcc tcttcgacat tgcgacaata
    12961 aaactctcaa aatgggttaa ggataaaaga tgtttagtga aatgttgctt gaagatgaac
    13021 tggatcggaa aacaacagag gctttgattc gtgtagcgga cgaacattcc cggtcgctta
    13081 tgagcgatcg agaggctcgt ctggctattc gtgccatatt cgaaactgcg caggggcttg
    13141 ttggtacaca agtgggtgaa gccattaaca tcgccatgtc tcagttcagt gaaggcagta
    13201 aaaagcctct gtttcctatg catttgatgc tggctggtgg cacggtgctt tatatctctg
    13261 tttgtctgga tagcaaccaa atcaatattc tcaacactgt gtcaggtaag tggaaagatc
    13321 cgattgtctg tgaaaccagt gaagaaactt tgaaaaaagc ggctcaattt gtacgtagcg
    13381 cactacttaa gggcgctaag aagttgtaag gagttctgat gacaacgatt gttgcaggca
    13441 tcgatatcga gtctacggga ctggatttcc ttgctggtca taaaattatt gaaatcgcaa
    13501 ttacccgcta tgaactggag acacagagac atattgatag tctggagatg cgttttaacc
    13561 ctcgcaggaa catagatccg aaagctcaag ccgttcatgg catttcatta gaacagctgg
    13621 cagctgaacc tttgttgtca aatcatgcca gcgaaattgg cgcttatatg gaggcatgta
    13681 gtgcgtgggt tgctcataac ggcgaagcgt ttgatatacc atttattaga cacgagtttt
    13741 cagggtatgg agtaagactg ccagacgttc ccgttataga tactatgtta tcgggattgt
    13801 gggccacaga agacggtaaa cgtcctcgcc ttgaagaatt ggccttctct cttggcttta
    13861 tatacgatca tgccaaagca catagtgcct tatatgacac aaacttaatg atgcaatgct
    13921 tctttaaggc acgtaataaa tacggatttt ttaaattacc ctctgaaatt gtgtaaaaca
    13981 aaagcctgct ttaaagagag tttgaagcag gctttctttt aaagaacagt cgcctttcaa
    14041 tcatttcctg cctgtattta atacttttcc gcctgatagg tttagtcaaa atgtagccat
    14101 cgaaacgcaa atgcaactaa acagaaggag acttacatga gttcggttga aaatgtaatg
    14161 acaaatgatg atctggatga gctgacagcc atgttgcaat cacttgatga accagtaaaa
    14221 aaagctgcac aggttgaaaa tactgatgat attgacgatc tgcttctcgg cctcgatgct
    14281 ggcgtagcca tgagttctga tgatgttgcc gaagaactgt tcaatgaaga aaaagcaggt
    14341 gatttcagct ccgctttaaa tgagttggag ttagctcatg agcctataaa cgtaattaag
    14401 gctgaaagtg ttgaatctgc cgaaaacgag ccagaacaat tgggatttat tgaggttgaa
    14461 gagtgtgttg aggttaatga tgaattaaaa gttcaacagt caaatgatag caatacaaat
    14521 aaaaaagctc gtactgcaag aggtcctcgt tttactttaa gtgataaaga tgatttgttt
    14581 ttcaataaag caggcttaga aaaagatatt ttcttagacg cttacgaaaa cgcgcctgtc
    14641 aaagcaaagg ataagatatt aaaccttctt aattggttta gcggaggtcc agatattagt
    14701 gtttacacgg taatttccat gagacacctt ctcacagaaa agaaggctac aagtaatagt
    14761 attaagattg ctttaatgag caatccagaa aaaccgtatc cgcttaacac tgcgtcaact
    14821 caggctgggc aaatgatggc tgtatttcca gctacaggaa ttgccgttag agacggtgga
    14881 aatctaacat tgaacgaaga atcaccgatc gttaagaagt ttgtcgcgga gtacactatt
    14941 ggatgacgtt cccctactga aaataaagcc catagagagc tttatagtgc tgggtaagct
    15001 aacccatacc cagcaccaca aaaacgcgcc agagagcttc ttgttcgcat ttctggcgcg
    15061 ttttatttga ttgccataca taaaatcaaa tgcaaaaata ggtgtatact tacctatcga
    15121 gaaagaagat gattgcagcc gaaaaaatca aacagcgaaa gcgcgacaac tctcttcgtg
    15181 acctctggag aacacctgat tggctgtttt ctgccattca acgttatctt ggagtgacat
    15241 ttgatgttga cgttgcctgc aacaaggaca acgcaaagct gcctaatttc ataggcattg
    15301 agcgtgatgc tttgaaatct gaatggggac agccaggtac aattgccttc ctcaatccac
    15361 cctactccaa aatctccccc tggattgatg ctgctatacg tgagcaggct cgcggagtta
    15421 caacagtgat gctaattcct caatctctcg atacaaagtg gtatgagcgt gcaacagagt
    15481 atgcgaatga gacgattatt ctgtctggtg gccgcgtagc gtttgtcgag cctgacgtca
    15541 atttgggtca ggtagaagta aacatcaacc ccggtggcag tatgctcgtt gtttttcgag
    15601 gattctgtca ggacgctggg cactctataa gcaagatccc tttggacgtc atgaaaagtc
    15661 tgggagggta tgatcctgcg aatgtgatca ggaaaaaaag accatcaaag aaggctgctt
    15721 agtttgttct ggcgtctgta at
//
LOCUS       DAPQZD010000113        15335 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPQZD010000113 DAPQZD010000000
VERSION     DAPQZD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.114"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            41..994
                     /gene="ompT"
                     /locus_tag="RO454_003400"
     CDS             41..994
                     /gene="ompT"
                     /locus_tag="RO454_003400"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDY0935187.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1226..1894
                     /locus_tag="RO454_003401"
     CDS             1226..1894
                     /locus_tag="RO454_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0935188.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1949..2323)
                     /locus_tag="RO454_003402"
                     /pseudo
     CDS             complement(1949..2323)
                     /locus_tag="RO454_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2330..2931
                     /locus_tag="RO454_003403"
                     /pseudo
     CDS             2330..2931
                     /locus_tag="RO454_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2947..3023)
                     /locus_tag="RO454_003404"
     tRNA            complement(2947..3023)
                     /locus_tag="RO454_003404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2987..2989),aa:Arg,seq:tct)
     gene            3294..4160
                     /gene="folD"
                     /locus_tag="RO454_003405"
     CDS             3294..4160
                     /gene="folD"
                     /locus_tag="RO454_003405"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDY0935189.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            4162..4374
                     /gene="ybcJ"
                     /locus_tag="RO454_003406"
     CDS             4162..4374
                     /gene="ybcJ"
                     /locus_tag="RO454_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDY0935190.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            4482..5003
                     /locus_tag="RO454_003407"
     CDS             4482..5003
                     /locus_tag="RO454_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDY0935191.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5039..6424)
                     /gene="cysS"
                     /locus_tag="RO454_003408"
     CDS             complement(5039..6424)
                     /gene="cysS"
                     /locus_tag="RO454_003408"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDY0935192.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            6598..7092
                     /gene="ppiB"
                     /locus_tag="RO454_003409"
     CDS             6598..7092
                     /gene="ppiB"
                     /locus_tag="RO454_003409"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDY0935193.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            7095..7817
                     /gene="lpxH"
                     /locus_tag="RO454_003410"
     CDS             7095..7817
                     /gene="lpxH"
                     /locus_tag="RO454_003410"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDY0935194.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7928..8257
                     /locus_tag="RO454_003411"
     CDS             7928..8257
                     /locus_tag="RO454_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935195.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            8353..8862
                     /gene="purE"
                     /locus_tag="RO454_003412"
     CDS             8353..8862
                     /gene="purE"
                     /locus_tag="RO454_003412"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDY0935196.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            8859..9926
                     /gene="purK"
                     /locus_tag="RO454_003413"
     CDS             8859..9926
                     /gene="purK"
                     /locus_tag="RO454_003413"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDY0935197.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(10460..11353)
                     /locus_tag="RO454_003414"
     CDS             complement(10460..11353)
                     /locus_tag="RO454_003414"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDY0935198.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(11350..12165)
                     /locus_tag="RO454_003415"
     CDS             complement(11350..12165)
                     /locus_tag="RO454_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDY0935199.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(12176..13435)
                     /locus_tag="RO454_003416"
     CDS             complement(12176..13435)
                     /locus_tag="RO454_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDY0935200.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(13445..15112)
                     /locus_tag="RO454_003417"
     CDS             complement(13445..15112)
                     /locus_tag="RO454_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDY0935201.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEKALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         3686 a         4148 c         3907 g         3594 t
ORIGIN      
        1 tacatactca atcattaaaa cgattgaatg gagacctttt atgcgggcga aacttctggg
       61 aatagtcctg acaaccccta ttgcgatcag ctcttttgct tctaccgaga ctttatcgtt
      121 tactcctgac aacataaatg cggacattag tcttggaact ctgagcggaa aaacaaaaga
      181 gcgtgtttat ctggccgaag aaggaggccg aaaagtcagt caacttgact ggaaattcaa
      241 taacgctgca attattaaag gggcaattaa ttgggatttg atgccccaga tatctatcgg
      301 ggctgctggc tggacaacac tcggcagccg aggtggcaat atggtcgatc aggactggat
      361 ggattccagt aaccccggaa cctggacgga tgaaagtaga caccctgata cacaactcaa
      421 ttatgccaac gaatttgatc tgaatatcaa aggctggctc ctcaacgaac ccaattaccg
      481 cctgggactc atggccggat atcaggaaag ccgttatagc tttacagcca gaggtggttc
      541 ctatatctac agttctgagg agggattcag agatgatatc ggctccttcc cgaatggaga
      601 aagagcaatc ggctacaaac aacgttttaa aatgccctac attggcttga ctggaagtta
      661 tcgttatgaa gattttgagc taggtggcac atttaaatac agcggctggg tggaagcatc
      721 tgataacgat gagcactatg acccaggaaa aagaatcact tatcgcagta aagtcaaaga
      781 ccaaaattac tattctgttg cagtcaatgc aggttattac gtaacaccta acgcaaaagt
      841 ttatattgaa ggcgcatgga atcgggttac gaataaaaaa ggtaatactt cactttatga
      901 tcacaatgat aacacttcag actacagcaa aaatggtgca ggcatagaaa actataactt
      961 catcactact gctggtctta agtacacctt ttaacaacgt taaatagatt ttctccccgg
     1021 tggtattgta tagccccggg gaatttgttt accgttaaaa gcaaacaata tcccctaatc
     1081 ttgtaagcat ttaacctgcg tcctggcgtg taacctctta ccgcaacaca agactcaata
     1141 tattcaaatt cgttatgccg tggcatcatt taaccccttg taattcatcg ctataattga
     1201 tttaattcac aaataaaact ataacatggt gaaactaatg aaaaaaaaca cagatgatgg
     1261 ggctaaaatt tacacaccac ttaccctaaa gctttatgac tggtgggttt tgggtgtatc
     1321 aaatcggctt gcatggggat gtcctacaaa ggaacacctt cttccacact ttctggaaca
     1381 tttaggtaac aaccatctgg atattggcgt tggaactggg ttttacctta ctcacgtacc
     1441 tgagagtagt ctgatatctt taatggattt gaacgaagct agcctgaacg cggcatcgac
     1501 aagggctggg gaatcaaaaa ttaaacataa aattagccat gatgtttttg atccttatcc
     1561 cgcggcgtta catggtcaat ttgattccat ttccatgttt taccttcttc actgcctgcc
     1621 tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg gcgcaggcct taactgacga
     1681 tggaactcta tacggagcca caattcttgg tgacggcgtt gtgcacaata gcttcggtca
     1741 aaaactgatg cgcatttaca atcagaaagg catcttttca aacacaaaag attccgaaga
     1801 aggcttaaca catatactct cagagcattt cgagaatgtt aaaaccaagg ttaaaggtac
     1861 tgtagtaatg ttttccgctt cagggaaaaa atagcatcca gacgcagcac gttcttgcgc
     1921 acgacgtgct gcggcataat cccaatgatt actctctgac agggttcaca ggccactcaa
     1981 tatcaggggc agttgatgta tcaactcggt tcagcaacac ccgatacatc ttccaggctt
     2041 ccagcaacga ggtttcttcc tccgtatcct ttatccatga tgtgccgttc cacttctgaa
     2101 actcccctgc cggggataac caggtgacat tttccggtaa cggaccgagt tcagaaataa
     2161 ataacgcgtc ccctgatgcc acgtcataaa ccgttttacc cagatggtct tcaacgagat
     2221 gccacgatgc ctcatcactg ttgaaaacaa ccacgaagcc agccggaata tctggcggtg
     2281 caatatcggt actgtttgca ggcaaacctg tatgaggtgg aatggcacaa gagacttaca
     2341 aaagaatcag gcaaaacgcc tgattgatga gagccccgaa ctactgaaat ctgtcgtcaa
     2401 atttgcgctg gtaactgttc tgagaaagtc gaacatcata aatctggagt ggcaacagat
     2461 tgacatgcag cgacaagttg ccagggtgga tccagaagat agcaaatcaa atcgcgccat
     2521 tggtgtggcg ctgaacgata ccgcctctaa agtgttgcgt gatcaaacag gcaagcatca
     2581 caaatgtggg tgtacatacc agggcggcta agcgagcaga tggaacatca atgcctgcgc
     2641 tcaggaagat gcgcattgac agcaagacag catggctatc agcttgtcgt cgtgcaggag
     2701 ttgaaaattt ccgtttccat gacctctggc atacccgggc aagcaggttg attccgtcag
     2761 gcgtcccatt atcagtgctt caggaaatgg gcggatggga gtccagagaa atggttcgta
     2821 gatatgctca ccttgcgcct aatcatttga ctgagcacgc gaggaaaata gacgacattt
     2881 ttggtgataa tgtcccactc tggaattata gaagaaataa agaaggcgta actgattgaa
     2941 ttttaatggc gcgccctgca ggattcgaac ctgcggccca cgacttagaa ggtcgttgct
     3001 ctatccaact gagctaaggg cgcattgata ccacggtgtg ctgtaatcgc gtgaattata
     3061 cggtcaaccc ttgctgagtc aatggctttt gaaccagttg ctgaacaagt gtacgaccgc
     3121 atcagatgta gctcactatg gcggcaaacc aacgacggtt atcatgatag gtgtgaatca
     3181 gccaggtaca gaaggtttta cttttcagaa aacgaaaact gacagcgcgc cacgcttctg
     3241 acaaaatagg cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag
     3301 caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag
     3361 ttcaggcgcg tattgcagcc ggactgcggg cgccaggact ggccgttgtg ctggtgggta
     3421 gcaaccctgc atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt
     3481 tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg ctggagctta
     3541 tcgatgcgct gaatgccgac aacaccattg atggcattct ggttcaactg ccgttaccgg
     3601 cgggtattga taacgtcaaa gtgctggaac gtattcatcc ggacaaagac gtggacggtt
     3661 tccatcctta caacgtcggt cgtctgtgcc agcgcgcgcc acgtctgcgt ccctgcaccc
     3721 cgcgcggtat cgtcacgctg cttgagcgtt acaacattga taccttcggc cttaacgccg
     3781 tggtgattgg cgcatcgaat atcgtcggtc gcccaatgag catggaactg ctgctggcag
     3841 gttgcaccac caccgtgact caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg
     3901 ccgatttgtt gatcgttgcc gtcggcaagc cgggctttat tcctggtgac tggatcaaag
     3961 aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg
     4021 acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt ccgggcggcg
     4081 ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc
     4141 atgatccaca gggtgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag
     4201 ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg
     4261 atagccgaag gcctggtgaa agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc
     4321 gtcgccggtc agacagtgag ttttgcaggt cacagcgtac aggttgttgc ctgatccctt
     4381 tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc
     4441 atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc
     4501 acgccgccgt tcccctatgc attggtttag ggttgggttc gaaagccatc cctccgcgtc
     4561 tgttatttgc cggaatcatc ctggcgatgc tgcccgatgc cgatgtattg tcgtttaaat
     4621 ttggcgttgc ttacggaaat gtttttggtc atcgcgggtt tacccattcg ctggtgtttg
     4681 cgtttgttgt cccgctgtta tgtgtgctga ttggacgtcg atggttcagg gctgggctga
     4741 ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttgctg gattcggtaa
     4801 ccactggcgg taaatgggtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc
     4861 cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccacg ccgtacgggc
     4921 atcaggtgat tatttctgaa ttgatgtggg tgtggttgcc ggggatgtta ctgatgggaa
     4981 tgttgtggtg gcgcagacga taaccggatg cgaaaactcg catccggcaa tagcgcaatt
     5041 acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa
     5101 gacggtcacg cgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct
     5161 gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgca ccgctttgca
     5221 ggaacgcttc cggttcttgc tccagcaggc ccaatacggc agaaagttta cgcaggtgag
     5281 acgccattgc attcgccgct gccatatctt ctgctttcag acggttcact tcacgcgcca
     5341 tatcaaacag tacggaatag gcttccggag tgttgaaatc gtcgttcatc gcctcgataa
     5401 agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttgtctgtg ccgcgcagcg
     5461 cagtgtagag acgttccagc gccgcacgcg cctgtttcag gttctcttcg ctgtagttca
     5521 actgactgcg atagtgaccc gacatcagga agtaacgcac agtttccgca tcgtagtatt
     5581 tcagcacatc acgcacggtg aagaagttac ccagcgattt agacatcttc tcgcggtcaa
     5641 ccatcaccat accggagtgc atccagtaat tcacatactg gccatcgtgc gcacaggtgg
     5701 actgggcaat ttcgttctcg tggtgcggga acatcaggtc agaaccgccg ccgtggatat
     5761 caaagtggtt acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc cagccaggac
     5821 ggcccgcgcc ccacggagac ggccagctag gttcgccctc tttcgacatt ttccacagaa
     5881 cgaagtccat cgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcgcct gcctgcagct
     5941 gatcgagatc ctgacgcgac agcacgccat atgtcggatc ggtcgggacg tcgaacatca
     6001 cgtcgccgtt gtcagccaca taagcgtgac ctttagcgat cagttgttca gtgagttcaa
     6061 taatttcggc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag
     6121 catcaaaatc tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc
     6181 cattttcatt ggcgcgtttg atgattttgt cgtcgatatc agtaatgttg cgcacatact
     6241 tcagcttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaggca acaaaggtac
     6301 gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacgtac atgccgactt
     6361 ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta
     6421 gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt
     6481 cccgcgcgaa tcagcataca ttgcgagatg atccgatcgt gcggttatgc tataacacca
     6541 ccctatatat gacccgaacc gggttgaagc accaatcaaa cggaacagga tgcaaaaatg
     6601 gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct
     6661 gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc
     6721 caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa
     6781 aaagccacca aagacccgat caaaaacgaa gcgaacaacg gtctgaaaaa tacccgtggt
     6841 acgctggcaa tggcccgtac tcaggcaccg cactctgcaa ctgcccagtt cttcatcaac
     6901 gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc
     6961 gtatttgctg aagtggttga aggcatggac gtggtagaca aaatcaaagg tgtggcaacc
     7021 ggtcgtagcg gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc
     7081 gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac
     7141 cggcgatcac cgccggtttt ctgcgttttt tagcagggga ggcccgcaag gccgatgcgc
     7201 tgtatattct tggcgatctg tttgaagcct ggatcggcga cgacgatccc aacccacttc
     7261 atcaccagat ggccgcagcg atcaaagcgg tatccgattc cggcgttccc tgttatttca
     7321 ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc ggcatgacgt
     7381 tattgccgga agaaaaggtg ctcgaacttt atggtcgtcg ggtgttgatt atgcatggcg
     7441 acacgctgtg caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct
     7501 ggctgcaaac gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa
     7561 tgcgcgcgaa cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc
     7621 aaaacgcggt ggtcagtgcg atggaaaaac atcaagtgca atggctgatc cacgggcata
     7681 cccatcgccc ggcggtgcat gaacttatcg ccaatcagca acctgctttt cgcgtggtac
     7741 tgggtgcctg gcatacagaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctaa
     7801 tccattttcc tttctgacca cgaatggaca gattctgaaa aagcaccgaa ctaacgcaac
     7861 ctgtatatct tttttaaaat cagcaaggta ataatctctg agtgaaataa caacagggaa
     7921 tattattatg caacgctgca acggattttt gatttctggc atcgccctta cggcaggagc
     7981 gccactgtgt attagctggt tcggtcttgt cggcttctat tttggcgcat tgtcactggt
     8041 agctgcggcg gtcatttcct ggatagtcta tttctggctc atttatttag tactcacccg
     8101 cccccataac caacgctttg tagaaagata tatattgatt gtcggtatat gctttttacc
     8161 gtggagcatt gtgctgatat gggcattctt tgccagttca gccagagcgc ataatcaaca
     8221 cgcagaacaa attaaaaact caacaactga cggttaattc cgcctacgca accgttttcc
     8281 tagcactctt tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg caccacagga
     8341 gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc
     8401 aaaagcgact gggctaccat gcagttcgcc gccgaaatct tcgaaatcct gaatgtcccg
     8461 caccacgttg aagtggtttc tgctcaccgc acccccgata aactgttcag cttcgccgaa
     8521 agcgccgaag agaacggtta tcaggtgatt attgcgggcg caggcggcgc agcgcacctg
     8581 cctggcatga ttgccgccaa aacgctggtg ccggtgctgg gcgtgccagt acagagcgcc
     8641 gcactgagcg gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg cattccggtg
     8701 ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg cgttactggc agcacaaatt
     8761 cttgcgactc atgataaaga actgcaccag cgtctgaatg actggcgcaa agcacaaact
     8821 gatgaagtgc tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg
     8881 gtaacgggca gttaggccgt atgctgcgtc aggcaggcga accgttaggc attgctgtct
     8941 ggccggtcgg gctggacgct gaaccggcgg cggtgccttt tcaacaaagc gtgattaccg
     9001 ctgagatcga acgctggccg gaaactgcat taacccgcga gctggcgcgc cagccggcct
     9061 ttgtgaaccg cgatgtgttc ccgattattg ccgaccgtct gactcagaag cagcttttcg
     9121 ataagctcca cctgccgacc gcaccgtggc agttacttgc cgaacgcagc gagtggcctg
     9181 cggtgtttga tcgtttaggt gagctggcga ttgttaagcg ccgcactggt ggctatgacg
     9241 gacgcggtca atggcgttta cgcgccgctg aaaccgaaca gttaccggct gaatgctacg
     9301 gcgaatgtat tgtcgagcag ggcataaact tctcaggtga agtgtcgctg gttggcgcgc
     9361 gcggttttga tggcagcacc gtgttttatc cgctgacgca taacctgcat caggacggca
     9421 ttttgcgcac cagcgtcgct ttcccgcagg ccaacgcaca gctgcaggcg caagctgaag
     9481 agatgttgtc ggcgattatg caggagctgg gctatgtggg cgtgatggcg atggagtgtt
     9541 ttgtcacgcc gcaaggtctg ctgatcaacg aactggctcc gcgtgtgcat aacagtgggc
     9601 actggacgca aaacggtgcc agcatcagcc agtttgagct acatctgcgg gcaattaccg
     9661 atctgccgtt accgcaacca gtagtgaata atccgtcggt gatgatcaat ctgattggta
     9721 gcgatctgaa ttatgactgg ctgaaactgc cgcttgtgca tctgcactgg tacgacaaag
     9781 aagtccgtcc gggccgtaaa gtggggcatt taaatttgac cgacagcgac acatcgcatc
     9841 tgaccgcgac gctggaagcc ttaatcccgc tgctgccacc ggagtatgcc agcggcgtga
     9901 tgtgggcgca gagtaagttc agttaacctg gtgctcattt gattgccgga tgcggcgtga
     9961 acgccttatc cggcctaccg ttccaacacc gcctgtagac ctgataagac gcaccagcat
    10021 cgcatcaggc atggtgtgcc gattgccgga tgcggcgtga acgccttatc cggcctaccg
    10081 ttccgacacc gcctgtagac ctgataagac gcgccagcat cgcatcaggc atggtgtgcc
    10141 gattgccgga tgcggcgtga acgccttatc cggcctaccg ttccaacacc gcctgtagac
    10201 ctgataagat gcgccagcat cgcatcaggc atggtgtgcc gattgccgga tgcggcgtga
    10261 acgccttatc cggcctaccg ttccgacacc gactgtaggc ctgataagac gcgccagcat
    10321 cgcatcaggc atggtgtgcc gattgccgga tgcggcgtga acgccttatc cggcctaccg
    10381 ttccgacacc gcctgtagac ctgataagac gcgccagcat cgcatcaggc tagatgtgcc
    10441 aattgcctgg tgcccatcac tacagcgaaa tacaagtccc cgcttcgccc gccagcgtct
    10501 cttcaatccg cgataacgcc ccaatccacg cgggtttacc acggcttctg acatagccac
    10561 tcaccgccgt taccttcggc cccatcgaac catcggcttt ggcaaatggc gctaactcat
    10621 ccggtgtggc atggcgaatg gcacgttgct gcggcgttcc ccagttttca tataccgcat
    10681 cagcatcggt gaggatcacc agtccatcgg cattaatctg ctcggcgagc aacgcagcgg
    10741 cgagatcttt atcaatcacc gcttcactcc ctgccccgtc ttccgtcaca ggcacaccac
    10801 cgccgccact gcaaatcacc acatgcccct ctttgagcaa caactcgatg gcttcgctgt
    10861 cgagaatttt acgcggttgc ggagacgcca ccacccgacg taaatattta ccatcacgtt
    10921 tcatctgcca gccgtaagcc gcttccagtg cctcttgttc ttctggctga taaaccggac
    10981 cgataaactt ctctggctgt aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca
    11041 gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa gctctgcgcc agcatatagc
    11101 caatcatccc ctggctttcc gccaccagta catccagcgg atacggttct acctctttcc
    11161 acgccagatt ctgtaacgcc agcagcccca cctgcggtcc gttgccgtga acaattgcca
    11221 gccgataaga acgggccagg cgcgccagcg cgggtacagc actggcaata ttgcgatatt
    11281 gattttttgc cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca agagccacaa
    11341 ccagtgtttt catggtttcc cttgtaataa ttgttggcca agccagaacc ccagcagcgt
    11401 atctgcccct gacgtatgcc ccagcgccag cagcgaatcg atcgccgctg cggtacgttt
    11461 cgggcaactc agcgcatgaa caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc
    11521 ataacgtaaa taactgacgc tcaccgcagt ggtaacgagt tgaagattgt cggaacaggc
    11581 aaaaaacggg cgaccggagc gcgcatctaa agcgccataa taccaggccg ccagcagcat
    11641 tccgctcagc gtgtcatcat gacttggcgt taatccgggg cctttaccca gccagtgccg
    11701 ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc
    11761 agcaggcagc ggatcgcttg ccgccaatgc cagtggcccg aaaagcccgg tttcctccgc
    11821 gcgttgcatc catgcaacgg caagcggttg aggatgcgca ggcggcgtaa tacgtagcaa
    11881 acaataacgc tgtggctgtt taaccgtgaa acgcccgaga cgaatacctt gtgccacaac
    11941 ctgtggtcgt tcattgccgc ataatccacc gcataacgca tcgaattgcg cgcggcgaag
    12001 cacccatccg ccggggccga aaccactccc ctgacgatgc agcgttaaca gttcgccgct
    12061 ttccgtcatc aggttaatcg cccgccgcca cactcccgct aaccgccagg actggcgata
    12121 attcggtgcg ctacggctgg caagcagagg atggatgatc gtcattgtgc gttcctcaac
    12181 caatccccat gctttcagcc agtgcttcca gcgcctgttc aaagcacgcc agcggtgccc
    12241 gcacggtgcc tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata ccggtattga
    12301 tgagtggcgt aatgccggtt tctaccacgc gacgaatgtc cagccccagg cacgccccct
    12361 ggaaatccca gccggggatc tgcaactgca tattgcgttc aaggtaaatt tccgccatct
    12421 cttcagatac cgctctggcc gcttccatgc cacccgcacc gacaaagcgc gttacgccag
    12481 gcgctgcgat cattgccgca cctccgatac caaaggtttc ggtaatcgcg ctatcgccca
    12541 tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta
    12601 caggcgcagt aaaccagcgt tcacccagcc cgcttacccg aatcccgaac atattgccgt
    12661 tgcgggtcat agccgtaacg atgctgcctg cgcggatcat cgcgcctgca tccatcgccg
    12721 ccttgcagta ggccatcgcg aggttgagga agaactgatc ggtcacgctg agaaaatcca
    12781 tcacttccgc aatgtgctgt ttatcatgat cgaggcgagc aatttgtggg gccagcgtac
    12841 gcattaacag tgctgaggaa gcaatattgc gttgatggaa ctcatcgccc atggtaatgc
    12901 cctgcgccat cattgccgtg agatcgatac cgcgctccat gcgccccagc gccgcgctta
    12961 atactggcat taacacatcg cgcatccagc gatggcgagt caggacatct tcgccgtaag
    13021 cgccaaaacg catcactttg ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt
    13081 cggtcacgtt ctcgaccacc agcatcggca tactggcaga agtaatgccg cccattggcc
    13141 cgacggcatt cacatggtga caaggaatga agttcacttc cccctgctcc agtattgcca
    13201 gcgcctgcgc ttcatctttc gcccagcctt cgaacagaca tgcgcccacg cacgcccctt
    13261 tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc
    13321 ccttgtttag ttcgctgata agtgaagaag ccggttgcac atccagccag tgtggacgag
    13381 cacgacgaat ttgttcgata accgcagcat tggcttgcgc cactgatgta aacatgggaa
    13441 ccccttattg caaacgttct aataaacgag ccagtttttt attgccgccg gcgactggcg
    13501 accattggta atgcacaacc ggtttgctgg cgctttgtag ctccagcgca aagctgcgta
    13561 atccgatgtt aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc tgtgtggcag
    13621 gcgagagcgg atgaattaac gccgcagcca gcaaggtggc ttccggtagc gaactcacta
    13681 ccgcgatccc cgcatcttcc agcgtggcga tttgctgcga gcggcattgc gggtcacgtt
    13741 cagtgcctgt caccgtggca atggcataca gtggttgatt gtctgaacgc gcggcacagg
    13801 ctttttgcca ggcgctcacc agcgaggcgg caggatcggc ggtcgcgccg aagccaatca
    13861 cgacatcaag cagcaacact cgcacttgcg gtttagcgcc gagatcggca attaacaggt
    13921 tgcgtaaggt cgggtcgatc atcggatggg gacgcccgac ggtgtaaaaa tcatcgccca
    13981 ggtcgaggat ctgatgtccg tcggcgtcca gcatcatgcc atgttgatgg gtatcgtcgg
    14041 cttccacgcc aaggtgtccg gcaagtaatc ccgccgcttc agcagccagc gtaccgccgg
    14101 tatacaaacc gcaaataaat ccgctgctga caggcgctat cgcgttacgt cgcgcagtga
    14161 cgcgtgaaag cagacaagcc agacgagcgg cctcatccag cgaggaggca aaccagacat
    14221 tctcgtcgcg ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc ggtttgccag
    14281 ttgctttcat ggcattaaca attttcagac gcacagtttc ggcaggtggt tttgaaacaa
    14341 atgccagcac ttcgcttttc tcgtctgcac tgagcatttc cagcgccgtt agcgcactga
    14401 tgccgcccac ttcacggctg agatcgcgcc cgccaagacc aatcgcgtga gtaattccct
    14461 cccctgccag cgcaatctgt gagcacagct cctgaatccc ggtaccggaa gcgcctatga
    14521 cgccaatgtt gccttccggc atcacattag caaaagccag cggcgtggca gcaatcatcg
    14581 acgtaccgca gtctggcccc atcaccagca agcctttttc ccgcgcgcgg gttttaagtt
    14641 ggatttcatc ttccagcgtg acgttatcgg agaacatcat cacgttgagg ttgcgatcca
    14701 gcgcctggtt cgccaactcc gccgcatact caccagccac tgaaatcagc gccagattgg
    14761 catcgggtaa tttctgactg gcactgtccc agcgacgcac ctgcgtcaac gcctggctgc
    14821 tgcctgaacc ctgcgccagt tgttttagcg ccttttcaag ctgctgcata accgcctgcg
    14881 cgatccccgc atccgccgct tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt
    14941 tattaaaatc gtcatgccag aaacctgtag tatctaataa cgctttattg gcgggcgtac
    15001 ccatcattac ggaaacatca tcaacatttt ctgattcgct gagttttcgt gaaataatca
    15061 ttaaactgac cgaatcctga aaacaccctt ttttaataaa ggcgtggatc ataacaactc
    15121 cttaaaaatt gcacccatgg caattgcacg agcagtgtta atgtggcgat tacggtatcg
    15181 atgacaacga gtaaataagg tgatgaatat cacacgggga ttattaacaa aagaattcaa
    15241 ataaccgcca gactattacg cgttattttt taataagcat ggttgtttta aaaaccaggt
    15301 taaaagcacg aagattatca aataaattct aacta
//
LOCUS       DAPQZD010000114        15079 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPQZD010000114 DAPQZD010000000
VERSION     DAPQZD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.115"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            34..110
                     /locus_tag="RO454_003418"
     tRNA            34..110
                     /locus_tag="RO454_003418"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Arg,seq:acg)
     gene            391..957
                     /gene="yqaB"
                     /locus_tag="RO454_003419"
     CDS             391..957
                     /gene="yqaB"
                     /locus_tag="RO454_003419"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDY0935202.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            954..1382
                     /locus_tag="RO454_003420"
     CDS             954..1382
                     /locus_tag="RO454_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDY0935203.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1455..3011
                     /gene="gshA"
                     /locus_tag="RO454_003421"
     CDS             1455..3011
                     /gene="gshA"
                     /locus_tag="RO454_003421"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDY0935204.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3161..3676
                     /gene="luxS"
                     /locus_tag="RO454_003422"
     CDS             3161..3676
                     /gene="luxS"
                     /locus_tag="RO454_003422"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDY0935205.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3727..4839)
                     /locus_tag="RO454_003423"
     CDS             complement(3727..4839)
                     /locus_tag="RO454_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0935206.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4836..5543)
                     /locus_tag="RO454_003424"
     CDS             complement(4836..5543)
                     /locus_tag="RO454_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HDY0935207.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(5801..7339)
                     /gene="emrB"
                     /locus_tag="RO454_003425"
     CDS             complement(5801..7339)
                     /gene="emrB"
                     /locus_tag="RO454_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDY0935208.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7356..8528)
                     /gene="emrA"
                     /locus_tag="RO454_003426"
     CDS             complement(7356..8528)
                     /gene="emrA"
                     /locus_tag="RO454_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDY0935209.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8655..9185)
                     /gene="emrR"
                     /locus_tag="RO454_003427"
     CDS             complement(8655..9185)
                     /gene="emrR"
                     /locus_tag="RO454_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDY0935210.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQGGVVLEAMS"
     gene            complement(9276..9611)
                     /gene="ygaH"
                     /locus_tag="RO454_003428"
     CDS             complement(9276..9611)
                     /gene="ygaH"
                     /locus_tag="RO454_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDY0935211.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9601..10338)
                     /gene="ygaZ"
                     /locus_tag="RO454_003429"
     CDS             complement(9601..10338)
                     /gene="ygaZ"
                     /locus_tag="RO454_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDY0935212.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10462..11646)
                     /locus_tag="RO454_003430"
     CDS             complement(10462..11646)
                     /locus_tag="RO454_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935213.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(11838..12830)
                     /gene="proX"
                     /locus_tag="RO454_003431"
     CDS             complement(11838..12830)
                     /gene="proX"
                     /locus_tag="RO454_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDY0935214.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12887..13951)
                     /gene="proW"
                     /locus_tag="RO454_003432"
     CDS             complement(12887..13951)
                     /gene="proW"
                     /locus_tag="RO454_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDY0935215.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13944..>15079)
                     /gene="proV"
                     /locus_tag="RO454_003433"
     CDS             complement(13944..>15079)
                     /gene="proV"
                     /locus_tag="RO454_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDY0935216.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDVELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3706 a         3889 c         4011 g         3473 t
ORIGIN      
        1 atcaatatca gcagtaaaat aaattttccc gatgcatccg tagctcagct ggatagagta
       61 ctcggctacg aaccgagcgg tcggaggttc gaatcctccc ggatgcacca tattctccgt
      121 aaccttcagc aatgaaggta tcgaagaggt agcggtatta accgcaggca ccagggagga
      181 taacgttgcg ccagcagcgg cccgaagggc gaacgccagt gagtcatcct cccggatgca
      241 ccatattatc caaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      301 tttcctgaat tttcttctcc ttctccatca gcgacaaaat caagcaatta cgctaaagta
      361 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      421 gatatggatg gcaccatcct ggataccgag cctacgcacc gtaaagcgtg gcgcgaagta
      481 ttagggcact acggtcttca gtacgatgtt caggcgatga ttgcgcttaa tggctcgccc
      541 acctggcgta ttgctcaggc gattattgag ctgaatcagg ccgatctcga cccgcatgcg
      601 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      661 cctcttgttg aagtggtgaa aagctggcat ggtcgtcgcc caatggctgt aggaacgggg
      721 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      781 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      841 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      901 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      961 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
     1021 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg agtgagtcat ccctgggttt
     1081 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1141 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctaccggc tggctgaaac
     1201 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1261 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1321 aagcgttacg ctatgttgca gttgcagcag ctaccgttca gggcatgatg tggtggcact
     1381 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1441 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1501 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1561 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttctgcactg acccataaat
     1621 ggattactac cgattttgcg gaagcattgc tggaatttat tacaccagtg gatggtgata
     1681 ttgaacatat gctgaccttt atgcgcgatc tgcatcgcta tacggcgcgc aatatgggcg
     1741 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1801 tggcacagta cggcacttct aacaccggtc gctttaaaac gctgtaccgt gaagggctga
     1861 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactataat ttctctttgc
     1921 caatggcatt ctggcaggcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1981 tttctgcggg ttatttccgc gttatccgta actactatcg cttcggttgg gtcattcctt
     2041 atctgtttgg tgcgtctcca gcgatttgtt cttctttctt gcaaggaaaa ccaacgtcgc
     2101 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2161 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtatcacc ttcaatgatc
     2221 tctacgagta cgtggcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2281 agattggtat tgagaaagac ggtaagcggc tgcaaatcaa cagcaatgtg ctgcagattg
     2341 aaaacgaact gtacgcgccg attcgtccaa aacgcgttac ccgcagtggt gagtcgcctt
     2401 ctgatgcgct gttacgtggc ggcattgaat atattgaagt ccgctcgctg gacatcaacc
     2461 cgttctcgcc gattggtgta gatgaacaac aggtgcgatt cctcgacctg tttatggtct
     2521 ggtgtgcgct ggctgacgca ccggaaatga gcagtagcga acttgcctgt acacgcgtta
     2581 actggaaccg ggtgatcctc gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2641 aaaccgcgca gttcccgtta ccgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2701 tcgcgcaaac gctggatagc attaacggcg gcgaagcgta tcagaaagtg tgtgatgaac
     2761 tggtcgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2821 ttgatactgg tattggcgga acaggcaaag cgtttgctga agcgtaccgt aatctgctgc
     2881 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt cgccgagcgc gaggcgtctg
     2941 aacgccgtca gcaggaaatg gaagccgctg ataccgaacc gtttgcggtg tggctggaaa
     3001 agcacgcctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     3061 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaacaaag
     3121 agttcagaaa attttaaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3181 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3241 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3301 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3361 tcttaacggt aatggcgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3421 ttatatgagt ctgattggta cgccagatga gcagcgtgtc gctgatgcct ggaaagcggc
     3481 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3541 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtaacattct
     3601 ggaacgtgac gtgcgcatca acagcaacga agaactggcg ctgccgaaag agaagttgca
     3661 ggaactgcac atttagtcag tttaagcaga agaggccggg ctggtgaaaa caccgcccgg
     3721 cttttgttac ctgtcagtca tccgatattc cacgctatgt gcttcatcag gttgaggaac
     3781 ttccagcctt gaggccatcc gcatcacgac cgcttcagga actgcatact cgcgtctggc
     3841 attttgctgg ttccattgcg cccatggcac ttccagataa acgattttta ctcgtgcgcc
     3901 gtaagccgta aacagactga tgagctggct gcgcagctgg cgggtaatgt tcgttgcatt
     3961 ccagatgaac ggctttttct ggcgtaaaaa aacgcgcgcc tcttcttttg cctgttgcac
     4021 aatacgcccg gtggcggttt tgtcgtccgg actggcattg attcgccgcc gcatatcatc
     4081 aaggctgata acgtccattc cctggcactg ttcactgata tagcgatctt tgcccatacc
     4141 cggtaagcca cacagcaaaa taacctcaaa aggttcagcc tcccagggaa caaaatcagg
     4201 agaactttgc tcatgagtca ggtagtgcca gcgtgcggaa tcagaaacga acgggcgcat
     4261 ttttccccag cattgttgct catggcaaaa cagctcgaac agatcgatgc gttccagcat
     4321 tgattgctga tccgggcttt ggcgaccaag cagatcggca cgagcaagca aagcgagcag
     4381 acgcgtgtcg atgcgcattg cagccgtgag caacaaacgc tccggttccg gacgttccag
     4441 taaccatagc ggtaacccat gcagacgcac cagtgcaaca atttgctcgc gcagcacgaa
     4501 cggcgttggg atatcgcgcc ataaaatttg tcgagccgtc agttcgcccc gacgagcatg
     4561 accaggagac tgaatacgtc cgttttcctg cacggtagtg ctgcgctttt caacatcatg
     4621 caataacgcc gccgcccata aaacttcctg ctgttgagcg gggagttgct ggaattctgg
     4681 cagagtaatg agggcgttaa gcaccatttc ggtatgtacg ccgacgtcgc cttcaccatg
     4741 atgctcaggg tcttgtggcg tgtgatgcat ctcctcaacc cagctaaaac gctgacgtag
     4801 cgccgaccag cgtttatcgt cggtaagttg ccaaatcatg actgttctcc ataaacgaac
     4861 tcatgggcca tccgcgcccg ttgccagtgg cgtttccagt gcacagtggt tttgacatga
     4921 tttttgcgca catacttgaa gacgttatgc gaaaagtcag cgacagaaaa cgcatcatga
     4981 tttcggctga caatgccttc cagcgtacag ggttgacagg tttgcgtatc ccagggatcg
     5041 aatgtgccgc gcgcattggt cagcgccaga aaatcacgtt gccagctttt ttcatcattc
     5101 cccggctgcg ggccggagat ttcgggaacg caggggaaat caaacagagc ggcgtaaaac
     5161 tgcacctctt cccagctcag ccacatatcc tggcagcgaa cggcaaacag gtaaaagtcc
     5221 tgttccagcg cgcggtattc aatggaatga acggcgtaga gattttcacc aaacaactcc
     5281 aggttgccga gatcgttttt cagcagttgc cagcgttggc gaattttata ggtccatgcc
     5341 gactgcgtag gcgtggcatg ggagcgggca aacacgccgt agcggttaag gcagttattt
     5401 tcgccatcga gtttttctgt gtgcaccaat tgcgtgatgg tctgtaaatc ctgccagtaa
     5461 tcggtgttaa tgcggtcatc gctggtggtg cctggagaga aggggtaatg ccaggttctc
     5521 ccgtatttac gttgggtatt cataataatc cattattttg tgtgtaataa atgtacgccc
     5581 gcgagggtgc gagttttggg gttaaaagaa aaatgtgagt atcacccgcg aaagcgaggg
     5641 cgaaggaaaa agagaattaa caacctgagc gaatgctcag aaccggctaa tggtgaatgg
     5701 aagagtttgg ggcggcgaaa agatacaccg aatgagtgta taaaacaacc gtttgcagaa
     5761 tgagcaaata aaaagggagc cgcagctccc tttggcaacc ttagtgcgca ccgcctccgc
     5821 cgccacctgc gccaaatggc ggtttagcaa accacaccag ccccagcagg acgaggaata
     5881 tcccggctga catccagaag atctcattgg cggaaataat cagcccctga ttggttatct
     5941 gctgggcaat ccagcctgat gcctgctgtt gcgtcatacc aagcccttcc agttgactgt
     6001 acatcgcctg agcattcggg ttgaacgggt ttaccgactc agtcaactgt gcatggtgca
     6061 gcgactcgcg gttggtccac atggtcgtgg ttatcgacgt gccgatagac cccgccagcg
     6121 ttcgcgtaaa gttggagagg ctcgatgccg ccgccagtcg ttccggtggc aaaccggaca
     6181 gcgtaatggt ggtcagcggc ataaagaagc aggccaccgc aaaaccctgg ataaactgtg
     6241 gccaggccga cgcgccaaaa tccatacccg gttcaaaggt ataggcacgc cagtagaagc
     6301 agacggcata cataataaag ctgaaggtta ccagtcggcg catatccagt ttatgcgcga
     6361 agcggccgat aatcggcgac aggatcaccg gaataatccc taccggcgca gaggccagcc
     6421 ctgcccacgt cgccgtgtaa ccgtagacct cctgcaacaa ctgcggcagc agaacaatcg
     6481 cgccgaagta gagcatatac gcgaggctga tacacaagca gccgatggtg aagttgcgcg
     6541 acttaaacaa cgacagatcg actatcgggt tatcgtcggt cagctcccag acaatcagga
     6601 agcagatagc caccaccgcc accacggtga ggatgataat ttcctgtgat gagaaccagt
     6661 ccagctcttt accgcggtcg agcataatct gcaggctgcc gataccaata accagcagtg
     6721 ccagccccac ggcatcaatc cgccgccgtt cggtgcgggt ttcacgtccg cgcagagttt
     6781 gcagtgtcat caacaccacc gccacgccaa tcggcacgtt gatgaagaat atccagcccc
     6841 agtggtaatt atcactgata taaccgccga ggatcgggcc gcaaattggc gcaacaatca
     6901 ccgtcatcga ccacaacgcc agcgcgatcg agcgtttggc tggcgggtag ttattcagca
     6961 atagactttg cgaaagcgga attaacggcc cggcgacaat cccctgaatc acgcggaaga
     7021 agatcagcat attcaggctg ctggagacac cacacgccca cgacgcaata gcaaaggcga
     7081 tggtggacca gaggaacagt ttcacttccc cgacgcgctt tgccagccag ccggtaagcg
     7141 ggatcgagat ggcattcgcc accccgaaag aagtgattac ccacgttccc tggctgagcg
     7201 atgagcccag attcccggcg atagtgggga tcgccacgtt agcaatggtg gagtccagca
     7261 cctgcatgaa tgtcgccagt gacagcgcaa tcgtcataat gaccagttgt gcgccttcca
     7321 gcggtttttg ctgttgcatc acacgcacct ctggattagc cagcgttagc ttttacgata
     7381 tcgtcgatca gtttattgac aggtgccagg ctgatttcac gcgcggtgct taccgcgacc
     7441 ggtgtggaac gtactttgtt tgccagtacc tgaccgtcac ggttagtggt attgacactc
     7501 accagcgtgg ataaaccgat acgcagcggg tattgctcca gctgtttctg gtccagttca
     7561 atacgcacag gcagacgctg aacgactttg atccagttac cggtggcgtt ttgcgccgga
     7621 agcagtgaga acgcgctacc tgtgcccata tccagaccaa ccactttacc ggtgtatttc
     7681 acatcatcgc cgtaaatatc ggtggtgatg gtgaccggtt gaccgatacg catattggca
     7741 atctgcgtct ctttaaagtt ggcatccacc cacatattgg tggctggaac gaccgccatc
     7801 agcggcgtcg ttgggctaat ttgcgcccca ggctgtaccg cgcggcggga gacataaccg
     7861 gtcatcggac tgacaatacg ggtacgctcc agcgccagcc aggcgttacg tacttcggtg
     7921 gcagcctgtt gtacggcagg ctgatcttcc agtttcgtcc ccagaatcat cgcctgattg
     7981 gcattgtatt gttgaatagc gacgtccagt tgcgcctggg cactggtgac ggcgtcgcgg
     8041 gcgtgttgca gctcttcgcg accaatcagg ttggcattgc ccagcggcac acggcggttg
     8101 tagtcgcttt gtgctttcgc gagggcgatt ttttgcacct caatattcgc ctgcaactgt
     8161 ttgctgttaa tcatcagctg gtgggtttgg cgaacgctgg aagccagtgc agttttggct
     8221 ttttcaaacg cctggcgagc atctgtcggg tcgagagtga ccagcacgtc gccttctttt
     8281 acaaaatcgg tgttatcggc ccagactttc gtcacgctgc cagacacctg agacataatt
     8341 tgaatctgat tccctgccac gtatgcgtca tcggtttctt cgaaatgacg cagtaccaaa
     8401 aaccaataaa tccctatcgc tacggcaata attataaaga gcaaggtgag aaggaggagc
     8461 agacgcttac gtttgccgct cttctttacc ggttgctgcg gggtttgagt ctccgcattt
     8521 gcgctcatat tgttctccac gatcttctta actcatcggc tgagccgacc tgcttgccaa
     8581 aaaggccagc atgttgcgat gctggccggt tattttttct tttataaatc tggatttttg
     8641 agcgagatga cgcgttagct catcgcttcg agaaccacac cgccctgttc catctggtcg
     8701 agacgggaga gcaatttgcg ggtgatttgc tcgagctggt ctttttctgt tgtgctgagc
     8761 gcggaccaga gttgatgcag gcagttatgc tgcggcggta aaacctcgcg caaaaactcg
     8821 tgaccttttt ccgttaattg cagatgcagg cagcggcgat cgttatcgct ttcacgacgt
     8881 tcgatccaac cgcgtttttc cagttcatcg gcaatacgcg tcgcgttggt acgggatgat
     8941 ccaagagcac aactcaattc agaaggctga atactgtggt tttcctgaga ctccagcgta
     9001 atcaacgcca taaacaacgt ctcgttaatc ccctgagcct tcagcatttt attgcggttt
     9061 tccagcagct tgctttgcat atgcatgcaa agacgggtca gaaggatctc ctgataagga
     9121 aaatcttcgt ggcggctggc gcgaaatttt agcatttgtt caatgggcgt aaacgaacta
     9181 tccatttggg tatgacctca ttaatttcag ccgatatagt aacgacagtg acaaataaag
     9241 taaatgtatt gttttagaaa aatgattctt gtggattata taatcgccat cactttccag
     9301 gcgagcccat aggccagcgc actaagcagt gttgggatga taatgctgcg tgttttatag
     9361 aaactggcac ccagtaccgc gaagccgacc agtgtgggca cgaaacggcg tgtatcgtgc
     9421 atcacttctg gtgcggtaga gacaaccagc agagcgcata tcgaggcgat gccaatggtg
     9481 tcgagcaaaa tacctaccgc gccacgtttg gttgggcggg cattacccac acgcaggcgc
     9541 agcggcagat agcggaagca ataattcgcc gcgccaacca gtaacccaag cagcagaacc
     9601 tcatagctca tcgggcgctc cttgccagaa tgcctggatt aacgcagtga ggcagccaca
     9661 gacaatgcct gccagaatgg cgacgggaat agaaaatagc gttacgcctg caagggcacc
     9721 aactaacgct gcggtaacgc aaagagattg tttgcgctgg aaagaggcga gcaggaaact
     9781 cataaagagt gccggaagca taaaacctaa tgctgcttca acggcgggat aaccttgcag
     9841 caagccgctg ccggagaatg cccctattac cgtaccaaat acccacgatg accatgaact
     9901 gaaggcaatg ccgatcatcc agttttcgct ccagcggcga ttattgcgta ccagttttgc
     9961 ggttgcggcg gcaaaaacct catccgtcag gccaaacgcc cacagggcgg ttttcgattt
    10021 ttgcagacgc tgaataatac ggctacgcag tgacgggcca tacaaaacat ggcgaacatc
    10081 cattgccatg acggtcagtg cagcaaccca caaactactc ccggctgcca gcatcgcggt
    10141 aatgacgaac tggctcgcgc ctgcataaat gatgcaggag aaaaaaacgc tttcgagagg
    10201 agagaatccc agacgggtcg cattcagacc gaacgcaaag gccaccggaa tataactaat
    10261 aacaatcggt aaactgtctt tgcatccttc catgaaggtc gccgaaccag gagcaggctg
    10321 tggagtaggg ctttccatag agtgtacgct taacgattgt taatattata aatagactga
    10381 atgaatatct taaccttatc agactgatgg gcttcttaac acccttataa gtgtaaagcc
    10441 acgaaaacgg ttgctgattg cttaatttgc ggcttcctga cgatgaaaac ctcgccacca
    10501 gaccagtaag ttaaccaggg caatcgtcgc gccagccaga cacacgccag cccaactgcc
    10561 atgttgccag gctgaggctg aaattagcga accagcggca ccgccaataa agtagctggt
    10621 catgtaacct gcggtcaggc gattacgcgc atcagggtgt attcgataaa ttaccgtctg
    10681 gttagtgata tgcacgccct gcacggtgag gtccagcacc aggatgccga taatcagcgc
    10741 cagtacggaa gtgtgaccaa accagattgc cagccatgaa agtaacagca gaagcagacc
    10801 gaaagttgtt gtgtggtgcg atttgccctt gtcggcaaaa ccgcccgccg gacgagcgcc
    10861 caacgctccg gcagctcccg caagtccaaa cagaccaatt acaccatcgc tgtagttaaa
    10921 aggtggagcg gcaagcaaaa aggccattga ggtccagaga atgctgaagt tggcaaaggt
    10981 caggcagccc agcaacgcgc gggtgcgcag gatcttatcg ctgataaaca tgctgaaaac
    11041 ggaaccaagc aactgtgggt agttgaggtg ggtttctgat ttcatttgtg gcagaccacg
    11101 ccataatgcc agcgccatca gtgccattaa caccgaggca acccaaaaga cggtgcgcca
    11161 gccgccgagg tttgccagca atccggcaac ggtccgtgcg agtaagatcc ccaatagcag
    11221 gccgctcata atggtgccaa ccactttgcc gcgtttgtcc ggtgaggcca gcgtcgctgc
    11281 cagtggaacc agaatttgtg ccacgaccga gaataaaccg gttaatgccg taccgaggat
    11341 catcatcgcc agtgactgac tgctggcagt gattaacata ccgcccgctg ccagtaaggt
    11401 catcgagaca atcaggcggc ggcgttcaaa catatcaccg aggggaacaa gaaacagcag
    11461 acctgcggca tagcccaact gcgcggcggt aacaataaag cccgccgaac tggcggaaag
    11521 ggaaaagtta cgcgcgatgg tgtcgagcaa tggctgggcg taatagttac tggctaccgc
    11581 cagaccggtg gcgatagaca tcagcacgat cagcgccggg ctaagctcat gattaggttt
    11641 agtcattatt tttgttcatc aggtgatgat taagctggaa atcaataata gcggatgttg
    11701 atgaatatgg gatgaaagat agattgttga aatgtgcggt gtggtttgta ggtatgataa
    11761 gacgcgccag cgtcgcatca ggcattgtgt acagagcggc ggatgaggca aaaacgcctt
    11821 atccgcccga ataaaaatta cttctgcgct gccagcgctt cattcaccca gccatcgaac
    11881 tgctgctggt gggctttgat ccaaccatca acgtggccct gaatatcgtc ttctgaggct
    11941 ttgccgtcat gcatgatggc gttctgggcg ttaatatctg ccactggtaa ctgcataatg
    12001 gcaaacagtt tcgctgctgc cgggtttttc tcggcccagg ctttgttggc aacgatatgc
    12061 atggtactga ccgggaagcc gtaattcgcg ccattcggca gtttggtatt ggcattttta
    12121 tcgcccggca gtgcggagaa cggcacctgc aaccagacca catctttccc cggtttcagt
    12181 tcgttactca cccagtacgg cgtccaggtg tagtaaaaca ccggcttacc ctctttgtaa
    12241 cgactgatgg tgtcagccat catcgctgcg tagttcccct gattatgcgt cacggtattg
    12301 gtcagtccat acgcggcaag ctggtggttg atcgcacctt cgcagcccca gcctgggtta
    12361 cagccggtta aatccgcttt accgtcgccg ttggtatcga acagtttggc gatcttcgga
    12421 tctttaagtt gcgcgatgtt ggtaatttta tactggtcgg cggttttctt atcgatcagg
    12481 taaccttgtg ccgcgccgtt aacaaatacc ccttcacgat aaaatttctt atcgccaccg
    12541 gcagcttcgt acatgttgtc atgcagtggc gtccagttca cggcggtgaa ggttgcatcg
    12601 ccggaagcaa gcgaggtgta gccaacgttg taatccactt cgctgggttt attgacggta
    12661 taacctaatt tctccagcgc gcggctgacc agcagcgtct ggaaggtttc ttcggtgatg
    12721 gtgctttgaa ctggattaac tgtaatgcct ttgcccggca ggtcggcagc aaaagtttgt
    12781 gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc tatgtcgcat tgttattcct
    12841 ttttttggca gggtgatatg cccggtgtta ccgggcaagt gcagagttac ttaatgaatg
    12901 ggcgggtcag cagaccgaca gggccagtgg tgtaccagcg acggttgccg cgactacgtg
    12961 agtcgcgccc aacggcctgc gtcaggcgat cgaggataat ggcgaggatc acaatcccga
    13021 cgccgccaac ggtggcaagc cccatatcca gacgaccgat accgcgaagt accatctgac
    13081 ccagcccgcc gacggcaatc atcgaagcga tgaccaccat agaaagggcc agcatcagcg
    13141 tctggttaac gcccgccata atggttggca tcgccagcgg taactgaact ttgaacagca
    13201 tctggctcgg gctggcaccg aatgagcgcg aggcttcaat cagatccgcc ggaacctggt
    13261 taattcccag aatcgtcaga cggataatcg gcggcagtgc aaagatgatc gtcaccacca
    13321 cgcccggcac gttgccgata ccaaacagca tgacgattgg caccagataa acaaacgcgg
    13381 gcgtggtctg catggcatca agcagtggac gaataatttt cgccgctcgc ggacttctcg
    13441 ccagccagat ccccaacggc aaacctatga cgatacagaa cagcagggcg gttaacacca
    13501 gcgccagggt aaccattgcc tgtgaccagg caccgattgc gccgatggca atcagcgaaa
    13561 ccagcgtcgc cacgcccatt ccgaccccgg aaatctgcca ggcgatgaga gcgaaaacga
    13621 taatcgccac cggcgcgggc atacccagca gcaattgctg gaaaccgttg aggatgtaat
    13681 caaccggaac gcgcacgccc tgaaagacgg gacgaaaatg ggtaacgacc cagtcgatcc
    13741 cttcagtgac ccaactgtcg agcgggatca gagttttatg gaacggatcg agaatattaa
    13801 aatgctcgac gtttggcgca ggcgtactgg tcagccagtc agcaccaccg ccgtcagtcg
    13861 gtgcagcagc cggtgtaccc caggcgtctg cggattgtgc agcactgtcc gccgctggcg
    13921 tggtatccca cggattattt tgatcagcca ttatttaccc cctcacgatc taaagcgcgc
    13981 agcagcattc ctttcgaaat gatgccgaca tactgttggt cctcgtcgac cacgggcacc
    14041 gcacagggtg cctgtccgac atgagagagc aactcgctaa gaggcgtttg tgcatcgacc
    14101 gctaacggcg catcaatcag cgccgcatca agaccttgct gctgcgttaa cgcggcttta
    14161 agcgaatcga tagacacagc gccgacaaac ttattaccgc gttcgatgac gtagccatat
    14221 tcgcgatctt catcctgcaa taatttcagt gccgaacgcg ggccgaagcc gggggtttta
    14281 cgaattaagc cgttcggtgt acgacgagca atatctttcg cactgaacac ctggctaata
    14341 tcgacgccac ggaagaaggt acgaacataa tcattcgccg gattattaag aatttcatcc
    14401 ggtgtaccaa cctgcaccac ttcaccattt tgcataatgg caattcggtc accaatacgc
    14461 atagcttcat cgagatcgtg ggaaataaag acaatggtgc gctgatgttt cgcctgtaat
    14521 tttaccagct catcctgcat ctcggtgcga attaatggat cgagtgccga gaaggcttcg
    14581 tccattaata atatatccgg attaatcgct aacgcgcggg ctaatcccac acgttgacgc
    14641 atcccgccag agagttcatc cgggtagctg tgggcataat tttccagccc gacctgacgc
    14701 agtgcatcaa gggctttttc ccggcgttct tcggcattaa ttccggccag ttccataccg
    14761 aacgcagtat tgtccagcac ggtcatatgc ggcattaagg caaaggactg gaagaccatc
    14821 gcaatctttt ttctgcgcac ctcacggagt tcgacgtcgg atattttggc aatatccaca
    14881 ccatcaatca gcacttgccc gcgggtgggt tcaatcaggc gattgagaag gcgtaccatt
    14941 gtggatttac ccgaaccgga taatcccatg atgacaaata tctcgccttc ttcaatggcc
    15001 agactggcgt cttttacgcc aagcgatagc ccagtttttt ccagaatttg ttcttttgaa
    15061 agtccttgtt cgatatatt
//
LOCUS       DAPQZD010000115        14982 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPQZD010000115 DAPQZD010000000
VERSION     DAPQZD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.116"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(42..2321)
                     /gene="maeB"
                     /locus_tag="RO454_003434"
     CDS             complement(42..2321)
                     /gene="maeB"
                     /locus_tag="RO454_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDY0935217.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2610..3560
                     /gene="tal"
                     /locus_tag="RO454_003435"
     CDS             2610..3560
                     /gene="tal"
                     /locus_tag="RO454_003435"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDY0935218.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3580..5583
                     /gene="tkt"
                     /locus_tag="RO454_003436"
     CDS             3580..5583
                     /gene="tkt"
                     /locus_tag="RO454_003436"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDY0935219.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5660..6703)
                     /locus_tag="RO454_003437"
     CDS             complement(5660..6703)
                     /locus_tag="RO454_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDY0935220.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6829..7404)
                     /gene="nudK"
                     /locus_tag="RO454_003438"
     CDS             complement(6829..7404)
                     /gene="nudK"
                     /locus_tag="RO454_003438"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDY0935221.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7472..9451)
                     /gene="aegA"
                     /locus_tag="RO454_003439"
     CDS             complement(7472..9451)
                     /gene="aegA"
                     /locus_tag="RO454_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDY0935222.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9657..11357
                     /gene="narQ"
                     /locus_tag="RO454_003440"
     CDS             9657..11357
                     /gene="narQ"
                     /locus_tag="RO454_003440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDY0935223.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11521..14634
                     /gene="acrD"
                     /locus_tag="RO454_003441"
     CDS             11521..14634
                     /gene="acrD"
                     /locus_tag="RO454_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDY0935224.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14733..14792)
                     /gene="ypfM"
                     /locus_tag="RO454_003442"
     CDS             complement(14733..14792)
                     /gene="ypfM"
                     /locus_tag="RO454_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDY0935225.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3502 a         3979 c         3919 g         3582 t
ORIGIN      
        1 taccctgaat attcagggta agcgtgagag ttaaaaaaga attacagcgg ttgggtttgc
       61 gcttcaacca cggccagcgc caccatgttg acgatacgac gtaccgatgc gatcggcgtt
      121 aacacgtgaa ccggcttcgc cacccccatc agcaccgggc cgacagtcac accttccgag
      181 ctggaaacac gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga
      241 atattagcgg aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc
      301 agcgctgcat cgccgtgcat ttcaccatca atcatcagtt ctggcgcacg ttccctgacc
      361 agttccagcg cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag
      421 tgcgacaaca gagccacacg cggctcaata ccaaaacgac gaacagtttc tgccgccatc
      481 aaggtgatct ccgccagctc ttctgcatca ggctcatcat tgacgtaggt atcggcaata
      541 aaggtgttac cactcggcaa cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg
      601 cgataaccaa agacattttt caccacgcta aagtgttcat gataatcgcc caccgtaccg
      661 caaatcattg catcggcttc gccacgctga accatgatcg cgccgatcac tgtcgggtta
      721 ctgatcagcg cacgctgcgc ctgctcctga gtgacaccgc gacgcttcat gatctggaag
      781 tattcggtcc agtactcctt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca
      841 acgcccgcct tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga
      901 ccaataagga tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgtagaacg
      961 cgcgcctctt ccccttctgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa
     1021 atcggcttca taaacaggtt ggttttgtaa acgaactccg tcagcttgtc gatgtagacg
     1081 tcgaaatcag caatcggacg cgtcgccacg cccgactcca tcgccgcttt cgccaccgca
     1141 ggagcgatct taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca
     1201 aagctcagat cctgatcgcc atacgctgaa gccaccactt cactctgttc cgcatgggcc
     1261 agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca
     1321 acgtccagcg cgccacggaa gataaacggg aagcaaagga cgttgttcac ctggttcggg
     1381 tagtcggaac gaccggtaca gatgatggca tccggacgca cttctttcgc cagcggcggc
     1441 agaatttccg gttccgggtt cgccagcgcc aggatcattg gcgcacgggc cattttcttc
     1501 accatttcct gggtcagcac tttcgggccg gaacagccga ggaaaatatc cgcgccttca
     1561 atcacatcat cgagggttcg tttgccgtca tccaccaccg catatgcggc tttggtttcc
     1621 gccatgtttg gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta
     1681 tgtttttgca gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg
     1741 ccagaaacca ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg
     1801 atggcggcag tgctgataat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc
     1861 atccgctcgc gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg
     1921 ttgatgccgc cgaaggtcgg ttcgagcgca gcgacgactt caataaattt gtccgggtcg
     1981 agttcatcaa cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc
     2041 ttgccttcca tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc
     2101 gtaccgttag agatcaccgc taccaggtta ccgcgcgcgg tatatttgta ggcttttaac
     2161 gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga
     2221 tcgcgctgcg ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat
     2281 tcatggaaat caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt
     2341 aacgttcaca aataaattgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt
     2401 accctgccgg agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt
     2461 tggtaataat catataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg
     2521 ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct
     2581 aacactttac ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca
     2641 ccactgtcgt ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg
     2701 ccaccaccaa tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa
     2761 tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg
     2821 cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc
     2881 gcgtgtcaac agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg
     2941 cgcgccatct ggtggatttg tatcagcaac aaggcgttga gaaatcgcgc attctgatca
     3001 agctggcttc gacctgggaa ggaattcgcg cggcagaaga gctggaaaaa gaaggtatta
     3061 actgcaacct gacgctgctg ttttcttttg cacaggcgcg ggcctgtgca gaagcaggcg
     3121 tttttctgat ttcgccattt gtcgggcgta tttatgactg gtatcaggca cgcaagccga
     3181 tggacccgta tgtggtggaa gaagatccag gcgttaaatc agtacgcaat atctacgact
     3241 actataagca acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac
     3301 aaatcctcgc cttaaccggc tgcgaccgac tgactatcgc accgaatttg ctgaaggagc
     3361 tacaggaaaa agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac
     3421 gcccagctcc catgagcgaa gcagagttcc gttgggagca caatcaggat gcgatggcgg
     3481 tagaaaaact gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc
     3541 ttcttgccgc caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc
     3601 aatgcgattc gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc
     3661 gcgccgatgg gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac
     3721 cctaccgacc caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg
     3781 atgctgctct acagtttgct gcatctgacc ggttacgacc tgccactgga agaactgaag
     3841 aacttccgtc agttgcattc gaaaacccct ggtcacccgg aaatcggtta tacgccaggt
     3901 gttgaaacca ccaccggtcc tcttggacaa ggtttggcga acgccgtcgg gctggcgata
     3961 gcagagcgta cgctggcggc gcaatttaat cagccggatc atgaaattgt cgatcacttc
     4021 acctatgtgt ttatgggcga cggctgcctg atggaaggta tctctcacga agtctgttcg
     4081 ctggcgggca cgctggggct gggcaagctg attggttttt acgatcacaa cggtatttcc
     4141 atcgacggtg aaaccgaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat
     4201 cactggcatg tggtccacga gatcgacggc cacgatccgc aggcggtgaa ggaagcgatc
     4261 cttgaagcgc aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc
     4321 tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg gcgcaccgct gggggaagaa
     4381 gaagtagctc tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa
     4441 gagatttatc acgcctggga tgcccgcgaa aaaggcgaaa aagcgcagca gagctggaat
     4501 gagaagtttg ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgtcgg
     4561 atgagcggcg gtttgccgaa ggactgggag aaaacgactc agaaatatat caatgagttg
     4621 caggcgaatc cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac
     4681 gggccgatgc tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc
     4741 atctggaaag gttctgtttc gctgaaggaa gatccggcgg gcaactacat tcactacggg
     4801 gtgcgtgaat ttggtatgac cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg
     4861 ccgtataccg cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg
     4921 gcactgatga aagcgcggca gattatggtt tatacccacg actcaattgg tctgggcgaa
     4981 gatggtccga cgcaccaggc tgttgagcaa ctggctagtc tgcgcttaac gccaaatttc
     5041 agcacctggc gaccgtgcga tcaggtggaa gcggcggttg gctggaagct ggcggttgag
     5101 cgccacaacg gaccgacagc actgatcctc tccaggcaga atctggcaca ggtagaacga
     5161 acaccggatc aggttaaaga gattgctcgt ggcggttatg tgctgaaaga cagcggcggt
     5221 aagccagata ttattctgat tgccaccggt tcagagatgg aaatcaccct gcaagcggca
     5281 gagaaattag cgggagaagg tcgcaatgtt cgcgtagttt ccctgccctc gaccgatatt
     5341 ttcgatgccc aggatgagaa atatcgggag tcggtactgc cttcaaatgt ttctgcccgc
     5401 gtggcagtag aagcgggtat tgccgattac tggtacaaat atgttggcct gaaaggggca
     5461 attgtcggga tgacaggtta tggggaatca gctccggcgg ataagctgtt cccgttcttt
     5521 ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg tactgggagt gaaaggtgcc
     5581 tgatggtgat cgccggatgc tgattgtcgg atgtgacgct ggtgcgtctt atccggtcta
     5641 catgtccccg ccattttgtt taacgggtga tccacaacgt gggccaggcg tctggcccat
     5701 gccagttatc gcaggtgggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa
     5761 agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg cccttttgct aaggtcacca
     5821 gttcacgcga tttttcatcg aatgttgcgt tcatcagttc cagttcattg gtctcctgac
     5881 cgttgttgaa cggcaaacgc aaccgtaccg ggcgcgaggc ctgcggcttt ttacgcgaca
     5941 caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagcg
     6001 ctttatcatc ggtcagcgca gtgacattca cctcacgacg taacggatca agcgaacagc
     6061 gcagaccatt catccgccag tttccataat ccagcaaatc gttacgttct tcgagtgaga
     6121 gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgct ggcggtacgc
     6181 tgagcggcgg ttcgtcccct ttctttatcc acgcggtctc actgccaacg cgcttttgct
     6241 gggcatcaat aaacaacaac gccgctttta agccactcag agaaatagtc tgattgccgt
     6301 cgcgtaaggt gatcgccctc ccttcctgaa tcatctgcaa aaacgcggtg atggttgccg
     6361 tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt aacgccaacg
     6421 gctcaccatc taacagcagc cgtggcgcta tctccccttc tgacgcatcc ggcgacttca
     6481 acccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg cggctcaggg
     6541 tcatcaccag tccattatga tcgcccgtat tgcgcgctac acagaaattt tgattattac
     6601 aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacatcg
     6661 gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatatcg catggacggt acgaccccag
     6721 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga
     6781 tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc agtccattaa
     6841 atgtgacatt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
     6901 tttaatcatc tccagcgcct ggctgaacgg cagctcaagc acttcaatat cttcatcttc
     6961 gacaccacca ccggcgttag cgcgctggct gtcactgtat tcggcgataa aaaagtggat
     7021 tagctcagtc acaccgcctg gcgacatata cagttcaaat aatttgcgga cttcgccaac
     7081 ttcatagccc gtctcttcaa tcgcttcttt gcgaatgcac acttccggtt cgtcgttatc
     7141 cagcagcccg gcgcaggttt caatcagttg cccgctttca ttgccattaa cccaggtagc
     7201 gacgcggaac tggcgaatca gaaccacggt ctttttcttc gcgttgtaca ggaggatcgt
     7261 cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg
     7321 cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
     7381 aacgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatcttac
     7441 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttacgccca
     7501 gccagtcaat gatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
     7561 ccgcaccacg cacggcgtca ccaccggcga agattttcgg attcgtggtc tggtaacggt
     7621 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
     7681 ccagccacgg catcccgtgc ggattgaagc caaacgccat aatcaccgca tcggctggca
     7741 tgacaaattc actgccttcc accggcactg gacgccgacg tccctgggca tccggctcgc
     7801 caagacgcgt gcgcaggaaa cgaatcccgc agacgtgacc ttgttcattc agctcaagcg
     7861 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc acgggcgttc ttcacctctt
     7921 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
     7981 catggcgcag cgcggtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
     8041 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagt cccatcacct
     8101 gtttagtgtt ggcgatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg
     8161 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
     8221 ccaaaagcga attcaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
     8281 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
     8341 gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca
     8401 cgccattgcg ggtcagaaca tctgcacagg ccagccctgc cggacccgca ccgataatcg
     8461 ccacccgctt gtccactttg gtgacatggc ttaagtcagg acgccaacct ttcgccaacg
     8521 cctgatctga aatgtagcgt tcaatgttac cgatagttac tgcgccgtgc tcatcgcgaa
     8581 tagtacaggc accttcacac aaacggtctt gcgggcaaac gcgtccggta atttccggca
     8641 gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
     8701 caatccactg cggtatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
     8761 cgcacttaag gcagcgcgag gcttcccgtt gtgcctgatc ggcacggaat ggcagataaa
     8821 tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atctggctcg ccacgcgcgg
     8881 gcgttgcctg catttgctcg actttactca ttaccggcat ttcttgcgcc gcggtactgg
     8941 catgccacgg ttgatgttcc tgacgtgcgg tgcgcaagcg gcgggatttc gccatgccgg
     9001 agagtgcggc gtcagtgacc agttgcagcg cgtccgccgg gcaattttcg acacaggcag
     9061 gaccgttttc gcgccctgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg
     9121 ccgcaacggg tgtcagaacg atttgcatcg taccaaatgg acaggccacc acgcaggatt
     9181 tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc
     9241 cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc acggcactac
     9301 gttgctgctg atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat
     9361 gttgctcgtc attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt
     9421 gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc
     9481 ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca atgttcatgt
     9541 gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga
     9601 ctaaattgcg tttcagcgaa ctggaagatt aatgattttt tgtggagaag acgcgtgtga
     9661 ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt
     9721 cgattctttc cacgggtatc gctctgctaa cgctggcgag cagtttacgc gacgctgagg
     9781 ctatcaatat tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa
     9841 gtggcagtcc acaactcaat gcacatcgcc agttatttca gcaggcactg cattcaccgg
     9901 tattaaccaa tctcaacgtc tggtatgtgc cagaggcagt aaaaacccgc tatgcgcatc
     9961 tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgacttg ccgtggtatc
    10021 aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact
    10081 acgctgaacg caaaatgctg ctggtggtgg caatttccct ggctggcggc atcggtattt
    10141 tcacgctggt cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc
    10201 agctggttac cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata
    10261 ccagcctgcc gaatgagctt ggcctgcttg caaaaacctt taaccagatg tcgagcgagc
    10321 tgcataaatt gtaccgttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg
    10381 aggccaagcg tcgtctggag gtgttgtatc agtgttcgca ggcgctaaac accagccaga
    10441 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcggctgaat
    10501 atctggagtt aaatgtcggt gacaactggc ggattagcga agggcagcca aacccggaat
    10561 tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggagaa ctgcactggc
    10621 aaaatagtca cgtttcatca tcagaaccgc tgcttaacag cgtttcgtcg atgctgggac
    10681 gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac
    10741 gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct tacttacgta
    10801 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    10861 tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta
    10921 ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata
    10981 cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca accctggcgc
    11041 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    11101 tgaagcacgc caacgccagc gaaatcgccg ttagctgcgt caccgcgccg gatggcaatc
    11161 acacagtcta tattcgcgac aacgggattg gcatcggtga accgaaagaa cccgaaggcc
    11221 attatggtct gaatatcatg cgcgaacgcg cggaaagatt gggtgggacg ctgacttttt
    11281 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctctgcg gagggtgagg
    11341 aaagtcagct gatgtaatgc ctcttattga ccaaagaata gtggcactta aggttcagta
    11401 taaaagggca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    11461 ccgctacagt gaagcaagcc aagcctacaa cgatacgcag aaacacgagg tcctctttta
    11521 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt
    11581 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
    11641 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    11701 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct
    11761 cagagcagcg gcaccggtca ggcatctgtc acgttaagtt ttaaagcagg caccgatccg
    11821 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    11881 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
    11941 gccttcgtct ctaccgatgg ttcgatggat aaacaggata ttgccgatta tgttgccagt
    12001 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
    12061 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    12121 aaagatgtca ccgatgccat tgaatcacag aacgcgcaga ttgcggttgg gcaacttggt
    12181 ggtacgcctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    12241 caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta
    12301 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    12361 ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc gaacgaaatg
    12421 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt tccacacgga
    12481 ctggaataca aggtggcgta tgaaaccacc tcttttgtaa aagcctccat tgaagacgtg
    12541 gtgaaaacgc ttctggaagc tatcgccctg gttttcctcg ttatgtatct gttcctgcaa
    12601 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    12661 tcggtgcttt atgccttcgg ctatagcgtc aacaccttaa ccatgttcgc gatggtgctg
    12721 gcgatcggtc tgctggtgga tgatgccatc gtggtggtgg aaaacgtcga acgtattatg
    12781 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    12841 gcactggtcg ggattgcgat ggtgctttcg gcggtatttg tgccaatggc cttcttcggc
    12901 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    12961 tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacactact taagccactg
    13021 aaaaaaggtg agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca gatgtttaac
    13081 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    13141 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    13201 tcgttcttac cgctggaaga ccgtggcatg tttactacct cggtacagtt gcccagcggt
    13261 tcaacccagc aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
    13321 gaaaaagaca acatcatgtc ggtgtttgcc accgtgggtt ctggccctgg ggggaacggg
    13381 caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    13441 ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaacaagat taaagaagct
    13501 cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc gggttttgat
    13561 atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgaaaccag
    13621 ttgctggcgc tggcagcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgac
    13681 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgct
    13741 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    13801 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    13861 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    13921 gctttcgcga cctcacgctg ggagacaggc tcgccgcgtc tggaacgcta taacggttat
    13981 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    14041 attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg gacggcgatg
    14101 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    14161 gtggtattcc tgtgtctggc tgcgctgtat gaaagctggt cggtgccgtt ctcggtaatg
    14221 ctggtcgtgc cgctgggggt tatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    14281 aacgatgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
    14341 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg
    14401 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    14461 ttcggcgtat tgccaatggc aaccagcaca ggtgccggtt ccggtggtca gcatgcggtg
    14521 ggtactggcg taatgggcgg gatgatttcg gccactattc tggctattta cttcgtgccg
    14581 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
    14641 aaaaaggcga catgccaatg tgtcgccctt ttcaactttc cgattaagaa cctgctcagc
    14701 gggttcttgc tgtttgtact ttgtctcagg aattacttac gaagcataac ttcgataaag
    14761 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    14821 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    14881 gggggacaaa ccttatgtaa atactatggt cctgcggtga tgatttgtat gtgatacacc
    14941 gcaacatttc gagatattca tacggcatct aatactgatt ta
//
LOCUS       DAPQZD010000116        14856 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPQZD010000116 DAPQZD010000000
VERSION     DAPQZD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.117"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(49..384)
                     /locus_tag="RO454_003443"
     CDS             complement(49..384)
                     /locus_tag="RO454_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935226.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     PFYINVTGL"
     gene            complement(415..774)
                     /locus_tag="RO454_003444"
     CDS             complement(415..774)
                     /locus_tag="RO454_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDY0935227.1"
                     /translation="MNRKLLALFMAAITLPVAAAQYVKPGAVLTLHPGQNNAEFNINA
                     STGDGSGVCNMEGTAQQIAAGESQKRRWVWNDNASECVAVISELTNGKASIMTRGCEG
                     YCGASAAGAMDGLFNKK"
     gene            complement(789..1316)
                     /locus_tag="RO454_003445"
     CDS             complement(789..1316)
                     /locus_tag="RO454_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935228.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(1328..1597)
                     /locus_tag="RO454_003446"
     CDS             complement(1328..1597)
                     /locus_tag="RO454_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0935229.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
     gene            1719..2207
                     /locus_tag="RO454_003447"
     CDS             1719..2207
                     /locus_tag="RO454_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935230.1"
                     /translation="MSSKRTVSAIPTHPEASVEEAKDWLTAEEFTHIEGMPGTARGAR
                     MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGEAFTLNDSDRQLSLW
                     VQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKLPEDERKRLFEKLNG
                     KV"
     gene            2562..2954
                     /locus_tag="RO454_003448"
     CDS             2562..2954
                     /locus_tag="RO454_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HDY0935231.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
     gene            3074..3535
                     /locus_tag="RO454_003449"
     CDS             3074..3535
                     /locus_tag="RO454_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935232.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WSLLVKMDEEMLCFFDSNGRTTMPRKAFSLRTGVTRRQLFPDAIYFIEREF"
     gene            3537..3989
                     /gene="radC"
                     /locus_tag="RO454_003450"
     CDS             3537..3989
                     /gene="radC"
                     /locus_tag="RO454_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDY0935233.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNHNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            4036..4965
                     /locus_tag="RO454_003451"
     CDS             4036..4965
                     /locus_tag="RO454_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDY0935234.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(4952..5566)
                     /locus_tag="RO454_003452"
     CDS             complement(4952..5566)
                     /locus_tag="RO454_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935235.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGEPNGIYRPEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWARE
                     DVAWMLQPENINAQINIQNQKYARLSAEDETETESGNASTAKNI"
     gene            5789..5986
                     /locus_tag="RO454_003453"
     CDS             5789..5986
                     /locus_tag="RO454_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDY0935236.1"
                     /translation="MNTNRILRKKEVLHLTGNSSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            5988..6455
                     /locus_tag="RO454_003454"
     CDS             5988..6455
                     /locus_tag="RO454_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935237.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            6543..7397
                     /locus_tag="RO454_003455"
     CDS             6543..7397
                     /locus_tag="RO454_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935238.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
     gene            7998..9371
                     /locus_tag="RO454_003456"
     CDS             7998..9371
                     /locus_tag="RO454_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935239.1"
                     /translation="MKIPKWVYDRITEITDYVVGDTQWAVTRRQTVRWFLAHIWLETD
                     DDGWTICTVRDIRSSYASLLGQCEISYQGECFMSTLTDFLPTLSDIEFRAGKASKDPE
                     KRRASAWRFNPLLPVCDESARGKLNLVDLDTGESVSFRALLQGNGKAPGHAIDVEKRQ
                     TALKQTEKDFLAKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGRIEGQYVTY
                     DHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFRQYDVDYRV
                     KSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYGWCGNSKKKALK
                     ILRWWNRYISPLKSALESLLERVHGAHRKSCSSPRNYHRYANEVGLILDLNSEVYQRE
                     KTHHHQYARNKALLAFMICGVEQAYIREVVSMNPGRVCMLDHDGVVATGALSLPDWRG
                     FTMKVKD"
     gene            9549..10173
                     /locus_tag="RO454_003457"
                     /pseudo
     CDS             9549..10173
                     /locus_tag="RO454_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            10368..13025
                     /locus_tag="RO454_003458"
     CDS             10368..13025
                     /locus_tag="RO454_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HDY0935240.1"
                     /translation="MDELLTLGCIALFFALVVVPILAIIALSRSTAVRSELATLRRRV
                     EVLEQRGVTEAPATVVTTVPQSVSQMVVPVETSSPAVNIVLSEPVADVTPESVDPWRP
                     QPKPQNEALPTPAVEQSSAFGGVMSSLVRWFMQGNPLAKLGILLLFLGFSFLLRYTVE
                     HSLFPLELRLVATSLFAIALLVVGWRLRHKQLVYALIIQGGATGTLYLTVFGAFWLWQ
                     MLPMTLAFALLVAICAASVGLAIMQKALSLAMLASIGGYLAPLLLSTGGGSHVALFSF
                     YLLLSVGILAISIWQHWRELNLLGLLFTFGIGGLWGLDGYRSEYYLSSQLFLIANTII
                     FGVLSVALSLRAQEKGKQIIDGVLLFASPLVGFGMQYAITRHMEYGPALSALGYGGFY
                     LALAWLALRRYPSLGRPLVLAALALGGAFTTLAIPLALSARWTAMAWALEGLGILWLG
                     VQQQQRRMSYSGTALLVLAVCSALWAQMNGMSALSLVLIFAVLSLSWLAAAWLWRNIQ
                     LQGSWVLLAGGLIFWIIALIGASQLVLKKDSLVLSGVLALMAISVWGWRIVSGRLAWW
                     ELDVSKWLLWPTMLVMLLSQISQHEIFAAGWQNLAWCLALPAAGALLWRDAETLPPRL
                     SRLAHLSLFWMILLALAAELFWFAQDLPWGMAAWGSGLMMAAGGLLIFLVHEAVHRQL
                     WPFRSWPALYASQAMIPVAAVLVVLLVLTNLQDGVVYRQTWLPLVNPLEEGAAFALLG
                     LIVFYRVSQRFFPVQISVCRPWPLIALLALSFWWLNGILLRALAWYGEVPWSAETLWH
                     SRLIQTCFALFWMLAALVVMLRATRRCSRREWLCGSVLLGIVIVKLMLVDSARGGGLA
                     RAVTFIGVAILVLIVGYFSPLPPKAGEEK"
     gene            13022..14417
                     /locus_tag="RO454_003459"
                     /pseudo
     CDS             13022..14417
                     /locus_tag="RO454_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
     gene            complement(14604..14741)
                     /locus_tag="RO454_003460"
     CDS             complement(14604..14741)
                     /locus_tag="RO454_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935241.1"
                     /translation="MGQHGKSLPSFDAPETLKGRKIEWEYENLTHGNKAYNGYVTAIL
                     N"
BASE COUNT         3516 a         3500 c         3989 g         3851 t
ORIGIN      
        1 aaaacatgaa ttatttcacc caaaaggaac ccggatgtta ttcagtgatt ataaacctgt
       61 tacgtttata taaaacggac ctttaccctc atcagttttc tgaatccggt aggtgattgg
      121 tagagaattt tgcaaatctg gttttgtaaa cgttaaatca gcagcgcact gataaacatc
      181 cagatctgat tcatggctaa ttgttctgac attttctaca gaaatacgca catccttatc
      241 cagtttcatc cctttctcga ttgtctttct tgcaatatcc ccgaccagat tttttgcatc
      301 atcactacta cattttggcg ttttctcgcc gcagccagcc agcaaaccag cagcaattaa
      361 taccagaagt attgctcttt tcatctgtag ttccttcgta aattaatgat acttttattt
      421 cttattaaat agaccatcca ttgcgcctgc cgcagaagcc ccgcagtatc cctcgcaacc
      481 acgtgtcata atgctggcct taccgtttgt cagttcacta attactgcaa cacactcgct
      541 ggcattgtca ttccagaccc agcgacgctt ctgactttct ccggcagcta tttgctgtgc
      601 tgtcccctcc atattgcata cgcccgaacc atcccccgtt gaggcattaa tgttgaattc
      661 agcgttattc tgtcccggat gcagggttag aaccgctccg ggttttacgt actgcgctgc
      721 tgcgactggt agcgttatgg ctgccatgaa taaggccaac agttttctgt tcattaatta
      781 attccctgtt aagtgatacg gatgtttttg gtgattttcc ccagtgccag cggtctgcgt
      841 ttttccacaa agaaataaac gacgttgaat gcagacttgc gatgattccc tcgaccaaga
      901 gagggccagt tgcggaagga ttcaatctgt tcttctgaat agggtgtgtc agagaaatca
      961 aacagcacaa gcaaggcttt gccgtcatgt ttcatcaatt tctttacttc tgtactgttg
     1021 acggtcgcag agacattgga acgtgccgca gttggtttac gtactgcaaa ctcaacggcc
     1081 acatcgtcaa tgacaaaatc aatgtaaccg tgcccggata cagttcctgg cagtttgctt
     1141 tttacttccg gtgagacgtt atctgcaaac caacccagca aatagcaacg taccagaggc
     1201 agtaattccc gctcgctaca ttcgttcaga cgcagggttt tgacgaactc cctgcgataa
     1261 agagaactga aaaaatactc aaagcattcc gttatttcag tgatggtggt tgccatggtt
     1321 gcatccttta cctgctatta ggtccatttt taagggcata cctttccggc cataatgttt
     1381 ccggtgtcgt atccagagcc ccggcaataa tgcgctcagc tctgggataa tgacgctcta
     1441 aggcgttatt taatgtccgt ggcgcaaggc cgtgactgcg tgacagtaac gccagcgtta
     1501 accctcgctt gtgaagtgct gccacaatat cggcacgatg ccagttccct gaacttcctt
     1561 tgatttgctt tttctgcgat gctgttgagt tacgcacagt attaactcca tggaatcagt
     1621 acatggatat taaatgcgaa taaaagatac ctttcaagtt cttttggtat ctatttattg
     1681 gttttcatat taacgcattg atatctggtg aaatttatat gagcagtaag agaacagttt
     1741 cggctatacc tactcatcct gaagcttctg ttgaagaggc taaagactgg ctgacagcag
     1801 aggaattcac tcatattgag gggatgccgg gcactgcgcg tggcgccagg atgcgcttgg
     1861 aaaagctggc atccctgcac ccggaaatac gtcgtaaaag gactgccggg aaaggtgtgg
     1921 tttaccatat tagcgcagcc acaatgtcgc ttgatgatga gagaaacagg gctaagggcg
     1981 aagcgtttac tctcaacgac agtgatcgcc agctaagcct ctgggtgcag ctgtatcgca
     2041 atatgtcgcc agcttcgcgg gcgcaactga tggagcaggc cctcaggctg gtaaaggacg
     2101 atctgatgtc tcaggggccc aatgtttctg aactggcggt acaaatcatg aagctgcccg
     2161 aagatgagcg taagcgcctc tttgaaaagc tgaacgggaa ggtgtgatag cgtcagtttt
     2221 ccgccatgcc agacagaagg aaccaccccg gaatcctgac ctgtctgaca tggcagaatg
     2281 gaaagtatgc catcacttaa taatgaataa aaacctattg ccagttattt cattgctgaa
     2341 ccacatatta gtttctcttt gttactattt gcataaaaag tatatttttg atataataat
     2401 attgctggtg aactatattt tacccagtca gtacgttgtc atgcacttac catttattag
     2461 gtgattaaac cccacctgtg ctggtgcaga cataacgtct tatcagaata tattgaacag
     2521 gaaaatcact gatgaatatt gtccatcagg aagggctagc tatgcccgtc gatcaggaat
     2581 taataaatat catattaaat gaagcgggaa atccaccgcc gcataaagcc aaaattacag
     2641 ccgtcagtct gttatttaaa gacctgagtt acagcgctga aaagggtggt taccatcctg
     2701 tcgaaatacg gattatttca cgcaatgatg aatggtattt tgattacatt accgacttca
     2761 gttatatggg gacggtatac cccgaacttg aaaaggaaat tgatatcagc tggagtcagc
     2821 aatatgtctt tcttgcttat gttggtgacc tgcctgttaa cgccggaagg gagctgtttg
     2881 aactctggca atccaacttt attcagtatc acggcatgaa catctacacc attaccgttc
     2941 tctgggaaag ctaatccccc tgtactaacc atcactaatt tatggggaat tatctgtgac
     3001 gactgacaga gggatacgtg cgtctcaaca gggcgaactg gactgtcttt gcggtgctta
     3061 ttgcgtggtt aacatgctga gctggctgta tgacggcagg gtgaaacgac gcccgttaat
     3121 gaactggctt attcagactt atcagcagcg ctggcctttg catgaatggc tgactgaagg
     3181 aattgaagaa gaccgcttag actggttaat agctcaggtg ttgcagaaag ggcattaccg
     3241 ccgccagttc ccggttgaaa tcaccagacc ctttgcaggg aagcgggggt taacagacgg
     3301 gcggctgttc agggaaatgc agcggtttct tgacgtcact gaccactctc gcctgattat
     3361 gctcagcgac cagtttcact ggtcccttct ggtcaaaatg gacgaagaaa tgctctgctt
     3421 cttcgacagc aacgggcgaa ccaccatgcc ccgtaaagct ttctccctgc gtactggcgt
     3481 aacccgccgc cagctgtttc cagacgccat ttacttcatt gaacgggagt tctgacatga
     3541 ccatgtttac ccagcgtgaa acccgtatcc ttgaccaggc gcgtgacatt attagccggt
     3601 actaccagcg tggggttcaa ctctgttccc ctgatgatgt tcgacggtgc gtgatggttg
     3661 agctggcacc gcttgaacat gaagagtttg gcatcatcct actcgataat cataatcagc
     3721 tgctgcaccg cgaaatcctg ttcaggggta cgctaaactc ggtcagcgtt cacccccgtg
     3781 aagttattaa gcgtgtgctg aaacataacg ccgccgccgt catccttgtc cataatcacc
     3841 ccagcggcga gcctgaaccc agccggtgtg acatccagtt gaccaaaaag cttcaggagc
     3901 tgctggaaat actggatgtg cggttacttg atcactttat cgtggcagga actgaaacgg
     3961 tgtcgatggc tgagcgagga ctggtgtgat gctgaaagtt acttggttta atgtacgtta
     4021 ttgcgggtta cgcgcatgag caggacagcg ttgcgtcagg tcgtgccggt ggtgcaaacc
     4081 accgtgttcg atgcgtatat tgtcggtctg gccccctcgg gacggcgagg tatcaccagc
     4141 ctgttaaacc gcagcgccag catacttaaa cacggtgcgg atgcagcaga ctatccgtgg
     4201 gaacaactta actacgccgc cgttgccaaa gtgcgcgcgg cactgctgga tgacggttac
     4261 gccgtgtcat cggtcaacat ggcgctgtca gcactccggg gcgtagcgca gacggccttt
     4321 aacctcaatt acatggatgc cgaaacgctg gcacgtatcc ggtcagttaa gcgcgtgagc
     4381 ggcgatgtcc agcgcaaggg aagggcgctg ggcaggcagg aaatccgggt actgattcag
     4441 gctgcaaaac agcatccgca atctgtacgg cgctgtcgcg atgtcgccat tgtgctgatg
     4501 ctttgcggaa cgggcctgcg ggccggggaa ctggttaaac ttgagcgccg ggactatgac
     4561 aacggcatcc tgacggtgag gcaagggaag gggcgtaaat accgtgaaat tcacgtggcg
     4621 gatgcggtgg acaaggctat tcgtgcgtgg ctgaaagttg gcgcagacga ggctgacagt
     4681 gcgttgttta ggcgaattca gcgcagtggt aaggtcgcaa gccaaccgct gactacgacc
     4741 gggttaacgg gtatcctggc tgagttacag caaacggcag gtatagcacg ttttaccccg
     4801 catgatatgc ggcgcacctt catcacccgt ttgctggagc agggcgtgga tatcaatacc
     4861 gtccgccagt tggcaggcca cagcgacata tccacgacga cgcgctatga ctgtcgtggt
     4921 gatactatga agatcagtgc cagcaagcgc atcagatgtt tttagcagtt gaggcgttgc
     4981 cagactcggt ttcagtttca tcttcagcac tcaaccgcgc gtacttttga ttctggatgt
     5041 taatttgtgc attgatattt tcaggctgaa gcatccacgc aacatcctca cgcgcccatt
     5101 ctttaccgtc agagagtcgt tttaaccggg gtttccatat tccatactca ttaagttttt
     5161 ctgctaccga agcatatttc tcacgatctg actcccccgt atgaagcttg tcaaaacgac
     5221 cttttacaag ctggtaaaca gtatagattt ccagtgcctt aggggacctg gccgggacta
     5281 tcatggggtg ttcctccggc ctataaatcc cgtttggctc tccctcattg agcaggtcat
     5341 taaacatgtg gttcacgcag gcactcagca catctgccgc tttctccgca gcgaatttat
     5401 ccgggctaaa acccgctccc gtcagtttcc gggcaatcag tgccagtttt tgccgggtat
     5461 cccgtcccag ataaagcttc ttctccagaa attcaaagac ctcgttactg cttttccgtt
     5521 cccggtggca tctggcgtca gccttgttgc tttggttacc aatcatagtc acctccatga
     5581 cagtgttttg tacaaaatat caccattgag cctcagtgaa aattgcttct ttatatgacc
     5641 gttctggaga cagaaataga tgatctcgaa tgacatccac attaaagatg cccttaacgt
     5701 gctcaacgaa ggcatcgttg agtgaatcct tgcgtttttt ggtggttgta aataacccgc
     5761 tcctgaaaca gctcaaagga gcacaagcat gaacaccaac cgtattttgc gtaaaaaaga
     5821 ggtacttcat ctgacgggga actcgtcagc gacgttgtac cgactcatct cgaaaggatt
     5881 ctttcctttg tcaaagaggc taacgggcga caatggccgg gcggtaggct ggctggagag
     5941 tgatatcaat aactgggtta acagccgtat gcaggcggga gaatgaaatg aaagagataa
     6001 aatcgaaaac cagttctgcg aaaaaaacgc tggttccttg gttcgaaact atggggaaac
     6061 aggttggttt atcacaagaa acgatatcag cagtgttaaa ggagcagcgt ttacggatac
     6121 gtaagcttaa ctacgcatct gcatatacag tgtccaatat tctcatacag gattgcggcg
     6181 gggccgatga catgtggagc aactacgcta aactggcgct tatccttgag cgttgtaaat
     6241 acattccctg cgggagcagg gcctgtgcat attgttctac cgctcagcaa ttacattatc
     6301 agggggtgga tgtaaccggg gaatacgctt tatatcagtt gcctgagtta tttttagatt
     6361 ttttagaaac aaacgacaaa aattcctctt atcattggga ttacaataag ttagttgagc
     6421 ggcatgaaga tgctcaggcg aggtcattcg ggtaatagcc atttctgtat ggtgaaaaac
     6481 tgacatatta ccctgccact gcattttggt ggcagggtaa tcaatacagc taggaatatt
     6541 aaatgaacga ttcttattac ttcagaacat ctgacccgga ttacattcgt cacgcaccgt
     6601 caatgcttga gattttaagt aaagcattcc ccactattat tacccagcag gaaagtgagt
     6661 atgaactgca gcgtcttaac catttactaa ataaactgaa aggcagcaat cgctgctaca
     6721 acatcattgc tcaaaaacta aggcagtgca gaaacggatc tccatgcaac agtcttatat
     6781 gcccgcattg ccagcgggaa cgcattctgg cgcaactggc tatgttgcat gtcttgccgg
     6841 ggaattcagc agaatacgtg ggagtggtcc tgtttttcaa caaggatacg caaactccgc
     6901 cgccatggaa gaacattggg gcgctcaggg cgcaaatcgg tcgttataag cagaggataa
     6961 gccgggtact taaccgcctg gggtacgccg gtccggccac aggcaccttc agtatgatgc
     7021 gacatatgcc tgatggccca gaagagcgta ttttctgggt tccacagttg tgtttgttcc
     7081 tgcccaatga cagtacgttg attaaggggc taaaagcgca tatgtcgcga agtggtgggg
     7141 cattcattga tgcatcgacg cttaacacac cagtgatcgt gctcaggtat aaagatcccg
     7201 cgagactgat ttcatgcgcc ctgaatccag tctggcacac agcagattac acactgactg
     7261 ataaagacgc tcttgttaag tcaagaatgg agctactgaa aggccggaca ctgttaaaaa
     7321 gtctgcttac cctggactca ctcggaacag gtgtcgtttc tttttcgttt gggcaacccc
     7381 cagggaaagt gcactgattt accggtaatg cagcaactgc aatgcacttt tgctgttgat
     7441 taaattgcag tggaacgacg aataacagcg gcaaccactg cattttcgtt gttgtaaaac
     7501 tgcaaagctt gccagtgatt ttgatggcgc tgatgcactt taacattcca actgtctcga
     7561 ttttaacgca accgtctgtt ttctgcaggg gctcacggat gcctctgcac acccgatcct
     7621 taccatcaac tgacgagtta gcgcatctgt gagggctttc tgtgtttctg tcagtgaata
     7681 ttctacacac aatgggggga gcggtcagcg agcaccattg ccattgagcg caacggagag
     7741 cttggggaca tgcagcacta cggaggcttc atctccttct catgtcatta aacgatacgg
     7801 cgaacgagcg cagcgagtga gggaaggcgt cagcctgaac agggggaaag atttgcgacc
     7861 ggagtggagc aaatcatggg cggagcccat cctcgcgact ggggttttga ctttcgaatt
     7921 gcccagcgtt gagtttggtt aatgtagtga gacgaggatg ctgagagggg accgtgagta
     7981 ctgaaaagga ttatccagtg aagatcccta agtgggttta tgaccgtatt actgaaataa
     8041 cggattatgt cgtgggggat acgcaatggg ctgttacccg gcggcagaca gtacggtggt
     8101 tcctggctca catatggctg gaaacagacg atgatggctg gactatatgc actgtccggg
     8161 atatccgtag cagctatgcc agtctcctgg gacagtgtga aattagttac cagggggagt
     8221 gctttatgtc cacgctaact gatttcctgc cgacactttc ggatattgaa tttcgtgcag
     8281 gaaaagcgag caaagacccg gaaaagcgta gagccagtgc atggcggttc aaccccctgc
     8341 tgcctgtgtg tgatgagtct gccaggggta agttaaatct ggttgacttg gatactggtg
     8401 agtctgttag ttttagagct ctgcttcagg ggaacgggaa agctcctggg catgcgatag
     8461 atgtagagaa acgccagacg gccctaaaac aaacggaaaa ggacttcttg gccaaagttg
     8521 ggcgggggcg catgagtata cagtttgtga aggaattgcg tagccgtgag ccggatcact
     8581 attaccgcgc aggaatacgc agccttaacc atctctacaa tggcaggatt gagggacaat
     8641 atgtcactta cgaccatcat tatcgtctga cgtttggagg gcggtattat gatcaagctt
     8701 tccagaatct gcccaatgag ttcaaagcta aatttcggac cgggttactc aactatgaca
     8761 tcgaagcgtg caatctggcc tgtctgaacc atctgttcag acagtatgac gtcgattatc
     8821 gtgtgaagtc atcgatatat aaaaccatga tggaacatac cgggctgacc cgtaaacagt
     8881 gcaaacagat ggtccatacc accacctaca gaattgggcg tgtgactatt ggtgttaatg
     8941 atggccttgg ggcaaaagtg tatggatggt gcggtaacag caaaaaaaag gcgctaaaga
     9001 ttttgcgttg gtggaaccgt tatatcagtc cattgaaatc tgcgcttgag tcattgctgg
     9061 aacgtgtgca tggcgcacac cgcaagagtt gttcatcacc gcgtaactat cacaggtacg
     9121 ctaatgaagt cggattaata ctggatctta atagtgaggt gtatcagcgt gaaaagactc
     9181 accatcatca atatgcgcgg aataaggcac tgttggcgtt tatgatctgt ggtgtggagc
     9241 aggcgtatat ccgtgaggtt gtcagtatga accctggtcg tgtctgcatg ctggatcatg
     9301 atggggtagt ggcaacgggc gcgctttcac taccggactg gcgtggtttc acgatgaaag
     9361 tgaaggacta gcttactttc ttgaatttaa ctatctgcga tctctgataa gggcgtatta
     9421 cgttgtcaat cataatggga tatgcgcatt ggaacgatgt gcatatccag taagtgatcc
     9481 atgtccgctt tgagctgtga gttcaacggg tcgatgcaac gctgtcccta atcaaggggg
     9541 acgttgccat gaaacgaaga actcgtatta actacacgcc agagcaaaag gtgattattt
     9601 gggacagata taagcaaggt gattccctgc atgatatccc ccagaatgtt cgacagattt
     9661 cactctacta tcatgcctac aatccaccag gccggtggct accgtcctcc cgtgcgaaag
     9721 cggcaccggt tagcgttttc gcttgatgaa aaagaggaga tatccagagg actggtagca
     9781 aaacgcatca tcagggacat cgctgctaaa ttatcaatag ctccctcaac gattagccgc
     9841 gagatcagga ggcacggcgg tgcaaaacaa taccgtgcag caaaagccga tcctgctgcg
     9901 tgggaaaatg ctctgagacc aaaaccttgc aagctaattg aaagccccac attgtgtaaa
     9961 atcattgcag agaagatgca tcaggactgg tcgccggaac aaattggcgg ttggctaaaa
    10021 cgctgttatc ccgataatca ggaaatgcat gtgtcacatg aaatgattta taaaacgctt
    10081 tttataccaa cccggggagc tttaaaaaaa gagctgcagc aatgcctcag aagcggaaga
    10141 gtagtgcgta gatccaaaac ctcatcactt aaagtaaaca gagatacatt ctggtcacga
    10201 atgcccggtt gttcgtgctt tgcgttttaa tcgcatttta cagtttctgc ctgcgtgcag
    10261 aggttgatgg tattttctga aaaagcggct cgctttgcta atgcatatgg cacaaataca
    10321 acagtttgtg tcattctgac ccatctttat accgcgacgg tggaaacatg gatgagctgc
    10381 taacccttgg ctgcattgct ttattcttcg cactggtggt ggttcccatc ctcgcgatta
    10441 tcgcgctcag ccgcagcacg gctgttcgtt ctgagctggc gaccctgcgt cgccgggtgg
    10501 aggtgcttga gcagcgcggt gtgactgaag cacccgccac agtggtaaca actgtgccgc
    10561 agagtgtttc gcagatggtt gtgcctgttg aaacgtcttc gcctgcggtt aacatcgtgc
    10621 tgtcggagcc tgtggctgat gtcacgcctg agtccgttga cccctggcgt ccacaaccga
    10681 agccgcaaaa cgaggcgcta cccacaccag cggtcgaaca atcttccgct tttggtgggg
    10741 tgatgtcgtc cctggtgcgc tggtttatgc agggcaaccc gctggcaaag ctgggtattc
    10801 tgcttctctt ccttggcttc tccttcctgc tgcgttatac ggttgaacac tccctgttcc
    10861 cgcttgagct acgtctggtg gcgacttcgc tgtttgcgat tgccctgctg gtagtgggct
    10921 ggcgattgcg gcataaacag ctggtttatg cgctgatcat acagggtgga gctacgggga
    10981 cgctctatct caccgttttt ggcgcattct ggctctggca gatgctgccg atgacgctgg
    11041 cgtttgcatt actggtggcg atttgcgcgg cgagcgtggg actggcgatt atgcaaaaag
    11101 cgctcagcct cgcgatgctg gcaagtatcg gtggctatct ggcaccgctg ttgctttcca
    11161 ccggcggcgg tagtcatgtg gcgctcttct ctttctacct tctgctttcc gtcggtattc
    11221 tcgccatcag catctggcaa cactggcgcg agcttaatct gcttggcctg ttatttacct
    11281 ttggtatcgg cggtctttgg ggactcgacg gctatcgatc agagtattac ctgagcagtc
    11341 agcttttcct gatagccaat acgatcattt ttggtgtgct gagcgtggcg ctatcgctac
    11401 gagcacagga gaaaggtaag cagattattg atggcgtgct gttgtttgca tcgccgctgg
    11461 tcggttttgg catgcagtac gccataaccc ggcatatgga atatggtccg gcgcttagcg
    11521 cgctgggtta cggcggtttc tatctggcgt tagcatggct tgcattgcgt cgctatccct
    11581 ctttggggag gccgctggtg ctggcggcgc tggcgctggg tggggcattt accacgctcg
    11641 ctatcccact ggcgctttcg gcccgctgga cggcgatggc ctgggcgctg gaaggtctcg
    11701 gcatcctctg gctgggcgtg caacagcagc agcggcgtat gagctatagc ggcactgcgc
    11761 tgctggtgct ggcagtgtgc agcgcgctgt gggcgcagat gaacgggatg agcgcgcttt
    11821 ctctggtgct aatctttgcc gtactcagcc tcagctggct ggctgccgcc tggctgtggc
    11881 gcaatattca gttgcagggt agttgggttc tgttggctgg cggtttgatc ttctggataa
    11941 tcgcgctcat tggggcatcg caactggtgc tgaaaaagga ctcgctggtg ctgtctggcg
    12001 tgctggcgtt aatggcgata tcggtctggg gctggcggat tgtctctggt cgtctggcct
    12061 ggtgggagct ggatgtcagc aaatggttgc tgtggccgac gatgctggtg atgctgctgt
    12121 ctcaaatttc acaacatgag atttttgctg cgggctggca gaatctggcc tggtgtctgg
    12181 cgttacccgc tgcgggggca ttgctctggc gtgatgctga gacgttgccg ccgcgcctct
    12241 ccagactggc gcacctttca ctcttctgga tgattctgct cgcgctggcg gcggagctat
    12301 tctggtttgc gcaagatctg ccatggggca tggcggcgtg gggctccggc ctgatgatgg
    12361 ccgcgggggg cctgctcatc tttctcgttc atgaggcggt tcatcgtcag ctatggccat
    12421 tccgaagctg gcccgcactc tacgcttctc aggcgatgat accggtagct gcggtattgg
    12481 ttgttctgct agtactgacg aatttgcagg atggcgtagt ttatcgccag acctggctgc
    12541 cgctggtgaa tccactggag gagggggccg cttttgcgct actgggactc attgtatttt
    12601 atcgcgtttc gcagcgcttt ttcccggtgc agatttcggt atgccgtccc tggccgctca
    12661 ttgcactgct ggcgcttagc ttctggtggc tgaatggcat cctgctgcgc gcattggcct
    12721 ggtacggcga agtgccctgg agcgcggaaa cactgtggca ttcgcggctg attcagacct
    12781 gttttgccct gttctggatg ctggcggcat tagtggtgat gctacgtgcg acccggcgtt
    12841 gctcacgtcg cgaatggctt tgtggatccg tattgttagg gattgttatt gtgaaactga
    12901 tgttggtaga cagtgcgcgt ggcggcggcc tggcacgcgc ggtcacgttt atcggcgtgg
    12961 caattctggt gctaatcgtc gggtattttt caccgttacc gcccaaagcg ggagaagaaa
    13021 aatgaaatgg atgaaagcgg tgttatgcag cgtgctactg agtactgcgg gcgcggcggt
    13081 cagcagcgat gaagtgaagg aagcgccaac ggactacgca acaggcgtcg cgctggaaac
    13141 cacgggggca ttcgccctgg tatcgcattt cgctgccgca ggtggtgtat cagagcactg
    13201 catggccgga tttgcgcgat gtgcgggttt ttaaccatct gggggatatg gtgccgtttg
    13261 cgctggtggc gcagaaaacc cagaccgtta cgccagagac agtggctctg cgtcttttcc
    13321 cgcttgatat gtcgccggtt ccgccgcgtg atgagggtca acgtagtggg gatgctgttg
    13381 ttttacgttc aaaaaacggt attgaaattc atttgcaaag cgatgatgtc actgcgctcg
    13441 ggcagagcta tttactgacg ttgccggaag agaggaaaga ctctctttca ctgacgcaat
    13501 tgcgtctgaa ctgggggacg ccgacgggca actggcaggg gaaagcctcg gtttatgtca
    13561 gccgtgattt acgctactgg cgacccgtgc aggaagatgc gccgttaatg gatctgacgc
    13621 gggatagcga tcgtctgaaa atggacgcca tcagcaccaa cctgacattg tcacttgaag
    13681 gaaatcgcta cctgctggtg atcctcaact cgcaaagccc ggcactgacg ttaaacagcg
    13741 tgagcgctat tgctgacagt aatgagccgg agtccgaacg tattgtgatt ggcgctcggg
    13801 cggataaggt gtcggatgat gaagccgtct ggcgatggac acagccgcag cccctgacat
    13861 cgttgcggat cgatctggaa aatgaaggcg ttttaccggt ggaactggtc tggcgcagcg
    13921 gcgaaaaaga accctggcag tcactgacaa aaacggtgtt gtatcgcctg gacgggaagc
    13981 gttcagaaga tattcgtctt cctggccagt tagttgaggc tgttcgaata cgtacaatta
    14041 atgcgcgatt gccggaggca ttgccagcat tgagcggtgc gcgcgacagt tatcaactgg
    14101 tttttaacac gcaaggcaaa ggtccttaca tgctggcgtg gggaaaccgg gcggcgaaaa
    14161 aggccgatgt tggactcgat atgttgatcc ctgcgtcgct gcgtaaaacg caggagatag
    14221 ataatctgcc gtgggctata ccgcaggaga gcgtaactct cggcggtgaa ttgcgactaa
    14281 cggcgacgtc ggcagccgaa caacaaagtc agtggaaaac gctgctggtg tggggggcgt
    14341 taatacttgg cgttgccgtt ctggcgttta tggcatggcg aatctggcga gaagttaaaa
    14401 aggacggtgc ggcgtaagcc ataaaaaagc acagttgcat agttcacggt gttgataaac
    14461 tgttacaaca gaggcgttta agctgaggcg gggaaataat ctatttgtga ataatatgtt
    14521 acttcactgg ggcgtgagtc atgggaatga ttttgatacc tgcgtttttt attttagcca
    14581 tcctgattat cgctatcttc atttcagtta agaatggccg tcacgtatcc gttgtatgcc
    14641 ttgttgccgt gggtgagatt ttcgtattcc cattctattt tacggccctt aagcgtttcc
    14701 ggagcatcaa aacttggtaa agatttacca tgttgaccca ttatgcggcc cctctggaga
    14761 ccactttcag gtattttctc tactgatatg tacacatgga gagatgtgat tgcaaattcc
    14821 catcagcatt actaccaaaa gaatcagaca ggaagc
//
LOCUS       DAPQZD010000117        14551 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPQZD010000117 DAPQZD010000000
VERSION     DAPQZD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.118"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RO454_003461"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="RO454_003461"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDY0935242.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1554..2876)
                     /gene="xylA"
                     /locus_tag="RO454_003462"
     CDS             complement(1554..2876)
                     /gene="xylA"
                     /locus_tag="RO454_003462"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDY0935243.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3242..4234
                     /gene="xylF"
                     /locus_tag="RO454_003463"
     CDS             3242..4234
                     /gene="xylF"
                     /locus_tag="RO454_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0935244.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4312..5853
                     /gene="xylG"
                     /locus_tag="RO454_003464"
     CDS             4312..5853
                     /gene="xylG"
                     /locus_tag="RO454_003464"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDY0935245.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5831..7012
                     /gene="xylH"
                     /locus_tag="RO454_003465"
     CDS             5831..7012
                     /gene="xylH"
                     /locus_tag="RO454_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDY0935246.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7090..8268
                     /gene="xylR"
                     /locus_tag="RO454_003466"
     CDS             7090..8268
                     /gene="xylR"
                     /locus_tag="RO454_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDY0935247.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8376..9200)
                     /locus_tag="RO454_003467"
     CDS             complement(8376..9200)
                     /locus_tag="RO454_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDY0935248.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9520..11550
                     /gene="malS"
                     /locus_tag="RO454_003468"
     CDS             9520..11550
                     /gene="malS"
                     /locus_tag="RO454_003468"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDY0935249.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11728..12981
                     /gene="avtA"
                     /locus_tag="RO454_003469"
     CDS             11728..12981
                     /gene="avtA"
                     /locus_tag="RO454_003469"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDY0935250.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13020..13493)
                     /locus_tag="RO454_003470"
     CDS             complement(13020..13493)
                     /locus_tag="RO454_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDY0935251.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13595..14443)
                     /gene="yiaJ"
                     /locus_tag="RO454_003471"
     CDS             complement(13595..14443)
                     /gene="yiaJ"
                     /locus_tag="RO454_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDY0935252.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         3690 a         3699 c         3571 g         3591 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagt tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcgccaccc gtgcggtaat
      241 ccagttgctg accgctaatg tccgccagca tttgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccctttcg cctggggatt attgtgtggt gtacgctcgc
      481 cagaaagata aggcagaaac caaactggct cggcactttc atcagcctgt cgagctgcag
      541 tgattaaagc agggacattg ctcaggccgg ttaatttcgc ggcccaatcc agacacgaag
      601 ccgcactcag cattacagac attaaatgcc aacgttgcgg taacgcatgg caaaagctat
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaacc gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgccgccat accccacgct ttcgcaactt
      841 caggtagcaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtggca agcctgcagc atgtcgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttatcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcacccgcg
     1141 attgcggaac tcgcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgtaacacc cgttgctgag catccagtaa ggttgcacca tgcatctgtc
     1261 cggcaatacc taatgcttta acgtcttgca gagaatgctg aacgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgcag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaatgcccgg tatcgctacc gataaccggg ccaacggact gcacagttaa ccgttatttg
     1561 tcgaacagat aatgatttac cagattttcc agttgctcct ggcgaccact ctgatgcacc
     1621 ggagacaaat tatgttcctg agcatatttg gctaaatctg ccagtgacat ttggcctttc
     1681 aggatttgct ggcctaattc actattccag ccggaataac gctgcgcgat gcgtttatcc
     1741 agctcgccat cttcaatcat gcgcgctgca attttcagcg ccaacgccat cgtatccatc
     1801 gcgccgatat gaccgtaaaa cagatcatat ttatcagtac tttgacgacg tactttggca
     1861 tcgaagttca gaccaccggt ggtgaaaccg cctgctttga gaatttcata catcaccagc
     1921 gcattctctt ccacactgtt cgggaactgg tcggtgtccc agcccagttg cgcatcgcca
     1981 cggttggcgt cgacagaacc gaacaggcca agcgcaatgg cggtggctat ttcatgatgg
     2041 aaagagtgac ctgccagcgt cgcgtggtta gcttcaatgt tcagtttaat ctctttttcc
     2101 agaccaaact gtttcaggaa gccatagacc gtcgcagcat cgtaatcata ttgatgtttg
     2161 gtcggttctt gcggtttcgg ttcgataagc aacgtgccct ggaaaccgat tttatgttta
     2221 tgctcaacca ccatctgcat aaagcggccc agctgttcac gctcctggcg caagtcggta
     2281 tttaacagcg tttcgtaacc ttcacgacca ccccacagga catagttttc accgcccaat
     2341 ttatgggttg cttccatcgc tgtaacaact tgcgttgccg cccaactgaa gacttcagga
     2401 tctgggttcg tcgccgcacc cgcgccatag cgagggtttg taaagcagtt agcagttccc
     2461 cacagcagct tcacgccgct ctcttcttgc ttgcctgcca ggacatcaac catttgcgca
     2521 aaattattga tgtactcttt taacgacgcg ccctcagggg aaacatccac atcgtggaag
     2581 caataaaatg gcacatgtaa cttgtggaaa aactcaaatg cgacatctgc tttacgcttc
     2641 gccaacgcca gtgcctcacc aggctgctgc cacggacgat taaacgcccc cacaccaaac
     2701 atatccgccc cgttccagca gaaggtgtgc cagtagcagg cggcaaaacg caagtgctct
     2761 tccatacgct tgcccaacac cagttcgtcg ggattgtagt gacggaatgc taacgggttt
     2821 gaggattttg agccttcata acgaacgcga tcgagctggt caaaataggc ttgcatattg
     2881 aactccataa tcaggtaatg ccgcgggtga tggatgatgt cgtaatattg ggcactccct
     2941 ttcagttgct caattatgtt atttcacact gctattgaga taattcacaa gtgtgcgctc
     3001 gctcgcaaaa taaaatggga tgatgaaact gggtaattcc tcgaagagaa aaatgcaata
     3061 agtacaattg cgcaacaaaa gtaagatctc ggtcataaat caagaaataa accaaaaatc
     3121 gtaatcgaaa gataaaaatc tgtaattgtt ttcccctgtt tagttgctaa aaattggtta
     3181 cgtttatcgc ggtgattgtt acttattaaa gctgtcctct aactacagaa ggccctacac
     3241 catgaaaata aagaacattc tactcaccct ttgcacctca cttctgctta ccaacgttgc
     3301 ggcacacgcc aaagaagtca aaataggtat ggcgattgat gatctccgtc ttgaacgctg
     3361 gcaaaaagat cgagatattt ttgtgaaaaa agcagaatct ctcggcgcga aagtatttgt
     3421 acagtctgca aatggcaatg aagaaacaca aatgtcgcag attgaaaaca tgattaaccg
     3481 gggtgtcgat gttcttgtca ttattcctta taacggtcag gtattaagta acgttgtaaa
     3541 agaagccaaa caagaaggta ttaaagtatt agcttacgac cgtatgatta acgatgcgga
     3601 tatcgatttt tatatttctt tcgataacga aaaagtcggt gaactgcagg caaaagccct
     3661 ggtcgatatt gttccgcaag gtaattactt cctgatgggc ggctcgccgg tagataacaa
     3721 cgccaagctg ttccgcgccg ggcaaatgaa agtattaaaa ccttatgttg attccggaaa
     3781 aattaaagtc gttggtgacc aatgggttga tggctggtta ccggaaaacg cattgaaaat
     3841 tatggaaaac gcgctaaccg ccaataataa caaaattgat gctgtagttg cctcaaacga
     3901 tgccaccgca ggaggggcaa ttcaggcatt aagcgcgcaa ggtttatcag ggaaagtagc
     3961 aatttccggc caggatgcgg atcttgcagg tattaaacgt attgctgccg gtacgcaaac
     4021 tatgacggtg tataaaccta ttacattgtt ggcaaatact gccgcagaaa ttgccgttga
     4081 gttgggcaat ggtcaggagc caaaagcgga taccacactg aataatggcc tgaaagatgt
     4141 tccctcccgc ctgctgacac cgatcgatgt gaataaaaac aacatcaaag atacggtagt
     4201 taaagacgga ttccacaaag agagcgagct gtaagcgtta cgccccggcg cggagcgggg
     4261 gcgtgatttc tctccatgcc gcgttaatta attggcttag gtggagtcgt tatgccttat
     4321 ctacttgaaa tgaaaaatat taccaaaacc ttcggcagtg tgaaggcgat tgataacgtc
     4381 agcttgcggt tgaatgctgg cgaaatcgtc tcactctgtg gggaaaatgg gtcaggtaaa
     4441 tcaacgctga tgaaagtgct gtgtggtatt tatccccatg gttcctacga aggcgaaatt
     4501 atttttgcgg gagaagagat tcaggcgagt cacatccgcg ataccgaacg caaaggtatc
     4561 gccattattc accaggaatt ggcgctggtg aaagaactga ccgtgctgga aaatatcttc
     4621 ctgggtaacg aaataaccca taatggcatt atggattatg acctgatgac gctacgctgt
     4681 cagaagctgc tcgcacaggt cagtttatcc atttcacctg atacccgcgt tggcgattta
     4741 ggacttgggc aacaacaact ggttgaaatt gccaaggcac ttaataaaca ggtgcgtttg
     4801 ttaattctcg atgagccgac agcctcatta actgagcagg aaacgtcggt tttactggat
     4861 attattcgcg atctacaaca gcacggtatc gcctgtattt atatttcgca caaactcaac
     4921 gaagtcaaag cgatttccga tacgatttgc gttattcgcg acggtcaaca cattggtacg
     4981 cgtgatgctg ctggaatgag tgaagacgat attatcacca tgatggtcgg gcgagagtta
     5041 accgcgcttt accctaatga accacatacc accggagatg aaatattacg tattgaacat
     5101 ctgacggcat ggcatccggt taatcgtcat attaaacgag ttaatgatgt ctcgttttcc
     5161 ctgaaacgtg gcgaaatact gggtatcgcc ggactcgttg gtgccggacg taccgagact
     5221 attcagtgcc tgtttggtgt gtggcccgga caatgggaag ggaaaattta tattgatggc
     5281 aaacaggtag atattcgtaa ctgtcagcaa gccatcgccc aggggattgc gatggtaccc
     5341 gaagacagaa agcgcgacgg catcgttccg gtaatggcgg ttggtaaaaa tattaccctc
     5401 gccgcactca aaaaatttac cggtagtatt agccagcttg atgacgcggc agagcaaaaa
     5461 tgtattctgg aatcaatcca gcaactcaaa gttaaaacgt cgtcccccga ccttgctatt
     5521 ggacgtttga gcggcggcaa tcaacaaaaa gcgatcctcg ctcgctgtct gttacttaac
     5581 ccgcgcattc tcattcttga tgaacccacc aggggtatcg atattggcgc gaaatacgag
     5641 atctacaaat taattaacca actcgtccag cagggtattg ccgttattgt catctcttcc
     5701 gaattacctg aagtgctcgg ccttagcgat cgtgtactgg tgatgcatga agggaaacta
     5761 aaagccaacc tgataaatca taacctgact caggagcagg tgatggaagc cgcattgagg
     5821 agcgaacatc atgtcgaaaa gcaatccgtc tgaagtgaaa ttagccgtac cgacatccgg
     5881 tggcttttcc gggctgaaat cactgaattt gcaggtcttc gtgatgattg cagctatcat
     5941 cgcaatcatg ctgttcttta cctggaccac cgatggtgcc tacttaagcg cccgtaacgt
     6001 ctccaacctg ttacgccaaa ccgcaattac cggcatcctc gcggtaggaa tggtgttcgt
     6061 cataatttct gctgaaatcg acctttccgt cggctcaatg atggggctgt taggtggcgt
     6121 cgcggcgatt tgtgacgtct ggctaggctg gcctttgcca cttaccatca ttgtgacgtt
     6181 ggttctggga ctgcttctcg gagcctggaa cggatggtgg gtcgcgtacc gcaaagtccc
     6241 ttcatttatt gtcaccctcg cgggcatgtt ggcatttcgt ggcatactca ttggcatcac
     6301 caacggcacg actgtttccc ccaccagcgc cgcgatgtca caaattgggc aaagctatct
     6361 acctgccagc accggcttca tcattggcgc gcttggctta atggcttttg ttggctggca
     6421 atggcgcgga agaatgcgcc gtcaggcttt gggtttgcag tctccggcct ctaccgcagt
     6481 agtcggtcgc caggctttaa ccgctatcat cgtattaggc gcaatctggc tgttgaatga
     6541 ttaccgtggt gttcccactc ctgttctgct gctaacgtta ctgttattgg gcggcatgtt
     6601 tatggcaacg cggacggcat ttggacggcg catttatgcc atcggcggca atctggaagc
     6661 ggcacgtctc tccgggatta acgttgaacg caccaaactt gccgtgttcg ctattaacgg
     6721 attaatggta gccatcgccg gattaatcct cagttctcga ctcggcgctg gttcaccttc
     6781 tgcgggaaat atcgccgaac tggacgcaat tgcagcatgt gtgattggcg gcaccagtct
     6841 ggcaggcggc gtcgggagcg tggccggagc ggtaatgggg gcattcatca tggcttcgct
     6901 ggataacggc atgagtatga tggatgtacc gaccttctgg cagtatatcg ttaaaggtgc
     6961 gattctgttg ctggcagtat ggatggactc cgcaaccaaa cgccgttctt gattttgata
     7021 aaaattttct caaagccggt aacgtattac cggttttgag tttttgcatg attcagcagg
     7081 aaaagaacca tgtttactaa acgtcaccgc atcacattac tgttcaatgc caataaagcc
     7141 tatgaccggc aggtagtaga aggcgtaggg gaatatttac aggcgtcaca atcggaatgg
     7201 gatattttca ttgaagaaga tttccgcgcc cgcattgata aaatcaagga ctggttagga
     7261 gatggcgtca ttgccgactt cgacgacaaa caaatcgagc aagcgctggc tgatgtcgac
     7321 gtccccattg ttggggttgg tggctcgtat caccttgccg aaagttaccc acccgttcat
     7381 tacattgcca ccgataacta tgcgctggtt gaaagcgcat ttttgcattt aaaagagaaa
     7441 ggcgttaacc gctttgcttt ttatggtctt ccggaatcaa gcggtaaacg ttgggccact
     7501 gaacgcgaat atgcatttcg tcagcttgtc gctgaagaaa agtatcgcgg agtggtttat
     7561 caggggttag aaaccgcgcc agagaactgg caacacgcgc aaaatcggct ggcagactgg
     7621 ctacaaacgc taccaccgca aaccgggatt attgccgtta ctgacgcccg ggcgcggcat
     7681 attctgcaag tatgtgaaca tctacatatt cccgtaccgg aaaaattatg cgtgattggc
     7741 atcgataacg aagaactgac ccgctatctg tcgcgtgtcg ccctttcttc ggtcgctcag
     7801 ggcgcgcggc aaatgggcta tcaggcggca aaactgttgc atcgattatt agataaagaa
     7861 gaaatgccgc tacagcggat tttggtccca ccagttcgcg tcattgaacg gcgctcaaca
     7921 gattaccgct cgctgaccga tcccgccgtt attcaggcta tgcattacat tcgtaatcac
     7981 gcctgtaaag ggattaaagt ggatcaggta ctggatgcgg tgggcatatc acgctccaat
     8041 cttgagaagc gttttaaaga agaggtgggt gaaaccatcc atgccatgat tcatgccgaa
     8101 aagctggaga aagcgcgcag tctgctgatt tcaaccacct tgtcgatcaa tgagatatcg
     8161 caaatgtgcg gttatccatc gctgcaatat ttctactctg tttttaaaaa agcatatgac
     8221 acgacgccaa aagagtatcg cgatgtaaat agcgaggtca tgttgtagcg atgatgagaa
     8281 ttgtcggcgt cgcttcaggt aactcagcac aaccacctga tgggagcaaa agccttatcc
     8341 gactgacaag tcggataagg ctcgaggaaa tgcaattaca tatgcgccgc gattaaccgt
     8401 tggttatcct ggtacattgc aaacaggtag ttgttataac tcttcccttt ggtcgagtag
     8461 cccttcagct tgtgaatcat cgctgtggca gtcacttcct gatccgcttt acgcagctgt
     8521 gcacgcgatt tacggaacga agagtaagcc gggtgcgtat tgaggtttgt aacataggcg
     8581 ctcaccgatt ctttaacaga actaaactgt gagtaccctt tcactttacc cggcgcattg
     8641 gtacaacgtc ctttcatgca tttcatgccg aaaagattgt tgttgttgcg cgccagcttt
     8701 gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca ccatactggt agggataatg
     8761 tctacgcgtt caagcaaggt attccacggg atttttcgcg tattaccaga ccacttcacc
     8821 ttgtagcgtt tggcgatgtc tttcagacgc gcacgctcag caggtgacca ttggccctga
     8881 tactgttttg aaatcagcca gttacgttcc gcagtaatgg ccgcattttg gctggtaatg
     8941 taaggcatta cagtccggag aaacgccttt ttccttggtg ttccggaagg gtattttcgc
     9001 aaatcaggaa gtgaactact ctttgcacta ttgcgagaat actcttgttt actgcttacc
     9061 tgtttattac tagctttagt taagtgggac ttttgactcg ctgttgttgt gtgcgtcttc
     9121 gctaacacct cactcgaaaa caccagagtg agtaacataa gaatcatcgc cccatatcgt
     9181 cgtatgggag tcaaaatcat caggtctcct ggtcggattt aatcattcca acaccttata
     9241 ttttttacaa atttgagagt tgaatctcaa atcatatcaa aaatagctgt caagagcacc
     9301 ccaaggaata gtccaaatct gaaactatgt cacgagttaa cgattcagat tggcgctaaa
     9361 tcgcagaaaa tgtggggttt atcgcaaaat tcagccgttt tttgcgcgcg atcgctcacc
     9421 cttgcttctc atcctgtgga gttaccgctc agggatgagt tttgtctggc ttatcgctgg
     9481 caaactgtct gaaatcgcag caataaggac tcatccgcca tgaaactcgc cgcctgtttt
     9541 ctgacactcc ttcctggctt cgccgttgcc gccagctgga cttctccggg gtttcccgcc
     9601 tttagcgaac aggggacggg aacatttgtc agccacgcac agttgcccaa aggtacgcgt
     9661 ccactaacgc taaattttga ccaacagtgc tggcagcctg cggatgcgat aaaactcaat
     9721 cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc aatggcgatt gttcagggac
     9781 ggcgaatata cgctgcaact agacacccga tccggtacac caacattgat gatttccatc
     9841 cagaacgccg tcgaaccggt agcaagcctg gtccgtgaat gcccgaaatg ggatggatta
     9901 ccgctcacac tggatgtcag cgccactttt gcggagggag ccgcagtccg ggattattac
     9961 agccagcaaa ttgcgatagt gaagaacggt caaataacgt tacaacccgc tgccaccagc
    10021 aacggtttac tcctgctgga acgggcagaa actgacacat ccgccccttt cgactggcat
    10081 aacgccacgg tttactttgt gctgacagat cgtttcgaaa acggcgatcc cagtaatgac
    10141 cagagttacg gacgtcataa agacggtatg gcggaaattg gcacttttca cggcggcgat
    10201 ttacgcggcc tgaccaacaa actggattac ctccagcaac tgggcgtcaa tgctttatgg
    10261 ataagtgccc catttgagca aattcacggc tgggtcggcg gcggtacaaa aggtgatttc
    10321 ccgcattatg cctatcatgg ttattacaca caggactgga cgaatcttga tgccaatatg
    10381 ggcagtgaag ccgatctacg gacgctggtt gatagcgcgc atcagcgcgg tattcgtatt
    10441 ctctttgatg tcgtgatgaa ccacactggc tatgccacgc tggcggatat gcaggagtat
    10501 cagtttggcg cgttatatct ttctggtgac gaagtgaaaa aaacgctggg tgaacgctgg
    10561 aacgactgga aacctgccgc cgggcaaacc tggcatagct ttaacgatta cattaatttc
    10621 agcgacaaaa caggctggga taaatggtgg ggaaaaaact ggatcagaac ggatatcggc
    10681 gattacgaca atcctggatt cgacgatctc actatgtcgc tggccttttt gccggatatc
    10741 aaaaccgaat caactaccgc ttctggtctg ccagtgttct ataaaaacaa aacggatacc
    10801 cacgctaaaa tcatcgacgg ctttacccct cgcgattact taacccactg gttaagtcag
    10861 tgggtccgcg actatgggat tgatggtttt cgggtcgata ccgccaaaca tgttgagttg
    10921 cccgcctggc agcaactgaa aaccgaagcc agcgctgcac ttcacgaatg gaaaaaagct
    10981 aaccccgaca aagcattaga tgacaaacct ttctggatga ccggtgaagc ctggggccac
    11041 ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg cgatgatcaa tttcgattat
    11101 caggagcagg cggcgaaagc tgtcgattgt ctggcgcaga tggatacgac ctggcagcaa
    11161 atggcggaga aattgcagga tttcaacgtg ttgagctacc tctcgtcgca tgatacccgc
    11221 ctgttccgtg aagggggcga caaagcagca gagttattat tattagcacc aggcgcggta
    11281 caaatttttt atggcgatga atcctcgcgt ccgttcggtc ctaccggttc tgatccgctg
    11341 caaggtacgc gttcggatat gaactggcag gatgttagcg gtaaatctgc cgccagcgtc
    11401 gcgcactggc agaaaatcag ccagttccgc gcccgccatc ccgcaattgg cgcgggcaaa
    11461 caaacgacac tttcgctgaa gcagggctac ggctttgttc gtgagcatgg cgacgataaa
    11521 gtgctggtca tctgggcggg gcaacagtga cttttccggc ttcccgttcg tgagtacctc
    11581 gggaagccgc caaccaggat aaaatgtcag ccctaatcag cattgcagga taaagccccg
    11641 ctcactcttc aacagaccga tttgcacccc agcaaatgta gcgttattgt taccttcctt
    11701 gctacagagt tcgacagata tcccgctatg acattctccc tttttggtga caaatttacc
    11761 cgccactccg gcattacgct gctgatggaa gatctgaacg acggtttacg cacgcctggc
    11821 gcgattatgc tcggcggcgg taatccggcg cagatcccgg aaatgcagga ctacttccag
    11881 acgctactga ccgacatgct ggaaagtggc aaagcgactg atgcactgtg caattacgac
    11941 ggtccacagg ggaaaacgga gctactcaca ctgcttgccg gaatgctgcg cgagaagttg
    12001 ggctgggata tcgaaccaca gaatattgca ctaacaaacg gcagccagag cgcgtttttc
    12061 tacttattta atctgtttgc cggacgccgt gccgatggtc gggtcaaaaa agtgctgttc
    12121 ccgctggcac cggaatacat tggctacgct gacgccggac tggaagaaga tctgtttgtc
    12181 tctgcgcgtc cgaatattga actgctgccg gaaggccagt ttaaatacca cgtcgatttt
    12241 gagcatctac atattggcga agaaaccggg atgatttgcg tctcccggcc gacgaatcca
    12301 acaggcaatg tgattactga cgaagagttg ctgaagcttg acgcgctggc gaatcaacac
    12361 ggcattccgc tggtgattga taacgcttat ggcgtcccgt tcccgggtat catcttcagc
    12421 gaagcgcgcc cgctatggaa tccgaatatc gtgctgtgca tgagtctttc caagctgggg
    12481 ctacctggct cccgctgcgg cattatcatc gccaatgaaa aaatcatcac cgccatcacc
    12541 aatatgaacg gcattatcag cctggcacct ggcggtattg gtccggcgat gatgtgtgaa
    12601 atgattaagc gtaacgatct gctgcgcctg tctgaaacag tcatcaaacc gttttactac
    12661 cagcgtgttc aggaaactat cgccatcatt cgccgctatt taccggaaga tcgctgcctg
    12721 attcataaac cggaaggagc cattttcctc tggctatggt ttaaggattt gcccattacg
    12781 accgagcagc tctaccagcg cctgaaagca cgcggcgtgc tgatggtgcc ggggcacaac
    12841 ttcttcccgg gactggataa accgtggccg catacgcatc aatgtatgcg catgaactac
    12901 gtaccagagc cggagaaaat tgaggcgggt gtgaagattc tggcggaaga gatagaaaga
    12961 gcctgggctg aaagccatta atacgctatt ccggcgttga gaaacgccgg aaaaacgttt
    13021 caaacaggct gctgccgtag ccgatgccgc tgcactttct cgacatccat gcactgcaac
    13081 gcctttgtcg ggcaggcttc aacacaggcc ggccccgtcg cccgatgcca gcacagatcg
    13141 cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc aaacgggcaa
    13201 gccagcatac agcttttaca gccaatgcaa cgtgattgtt caacgaaaat atgcccatgt
    13261 tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc acactgatga
    13321 caggctactg ccgtggtcca gctgtggtct ttaatgacac gaatacggga aataaactcg
    13381 tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca agccacttca
    13441 caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt catgatcact
    13501 cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg atccaacgaa
    13561 ggaaatgtta aagttttggc acttgtccaa agggttatgt aatcgcgccc tggtcgtccc
    13621 tgacagtaaa tcccagctca ttagaaatag cctgcgcggt ttcacgcagt ggtttcagga
    13681 gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac acggcgtacg
    13741 gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg agttcgtttt
    13801 cttctctgtc catcgccgct ccactttcac gaatgtgcgc cagttcgtcg aacatcgcgg
    13861 gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg ctttcccagt
    13921 atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt gcggaacagt
    13981 agagcggcat atgctggcct atataggcac gggttcgcag catcccggtt gtgggttcca
    14041 gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg gtttcaccag
    14101 tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc agcgaagata
    14161 gcgccttctg cccgacggca ataaatttgg tggtcaggcg ataactccct gcggcgggcg
    14221 cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg gtactcttat
    14281 ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag ttgctcaaaa
    14341 tctcaatcag catcaaccca cgaaacagac tctggcttcc ggctggacgc tctttttcct
    14401 gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg cgtcctgatg
    14461 gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc acttgatttt
    14521 taagtttttt gaaattcatc aatcagattg c
//
LOCUS       DAPQZD010000118        14349 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPQZD010000118 DAPQZD010000000
VERSION     DAPQZD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.119"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(65..406)
                     /gene="trbA"
                     /locus_tag="RO454_003472"
     CDS             complement(65..406)
                     /gene="trbA"
                     /locus_tag="RO454_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDY0935253.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGLNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTMPIITKFDSFRRMFICIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(420..1163)
                     /gene="traF"
                     /locus_tag="RO454_003473"
     CDS             complement(420..1163)
                     /gene="traF"
                     /locus_tag="RO454_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDY0935254.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1156..1413)
                     /gene="trbE"
                     /locus_tag="RO454_003474"
     CDS             complement(1156..1413)
                     /gene="trbE"
                     /locus_tag="RO454_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDY0935255.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(1440..3248)
                     /gene="traN"
                     /locus_tag="RO454_003475"
     CDS             complement(1440..3248)
                     /gene="traN"
                     /locus_tag="RO454_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDY0935256.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGKIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3245..3883)
                     /gene="trbC"
                     /locus_tag="RO454_003476"
     CDS             complement(3245..3883)
                     /gene="trbC"
                     /locus_tag="RO454_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDY0935257.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDEVVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPVLVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3892..4884)
                     /locus_tag="RO454_003477"
     CDS             complement(3892..4884)
                     /locus_tag="RO454_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDY0935258.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPIDALFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGHSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4881..5513)
                     /gene="traW"
                     /locus_tag="RO454_003478"
     CDS             complement(4881..5513)
                     /gene="traW"
                     /locus_tag="RO454_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDY0935259.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5510..5896)
                     /gene="trbI"
                     /locus_tag="RO454_003479"
     CDS             complement(5510..5896)
                     /gene="trbI"
                     /locus_tag="RO454_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDY0935260.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(5893..8520)
                     /gene="traC"
                     /locus_tag="RO454_003480"
     CDS             complement(5893..8520)
                     /gene="traC"
                     /locus_tag="RO454_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDY0935261.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKKNKARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHETVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8680..8901)
                     /gene="traR"
                     /locus_tag="RO454_003481"
     CDS             complement(8680..8901)
                     /gene="traR"
                     /locus_tag="RO454_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDY0935262.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPV
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(9036..9551)
                     /gene="traV"
                     /locus_tag="RO454_003482"
     CDS             complement(9036..9551)
                     /gene="traV"
                     /locus_tag="RO454_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDY0935263.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPCLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9548..9799)
                     /gene="trbG"
                     /locus_tag="RO454_003483"
     CDS             complement(9548..9799)
                     /gene="trbG"
                     /locus_tag="RO454_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDY0935264.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(9792..9953)
                     /locus_tag="RO454_003484"
     CDS             complement(9792..9953)
                     /locus_tag="RO454_003484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDY0935265.1"
                     /translation="MAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRII
                     QKYKEPFDE"
     gene            complement(9975..10112)
                     /locus_tag="RO454_003485"
     CDS             complement(9975..10112)
                     /locus_tag="RO454_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDY0935266.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFIHAVLSNCATRIVLPAPEKFG
                     S"
     gene            complement(10099..10689)
                     /locus_tag="RO454_003486"
     CDS             complement(10099..10689)
                     /locus_tag="RO454_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDY0935267.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(10679..12106)
                     /locus_tag="RO454_003487"
     CDS             complement(10679..12106)
                     /locus_tag="RO454_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDY0935268.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVMYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGGVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLRMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(12106..12810)
                     /gene="traK"
                     /locus_tag="RO454_003488"
     CDS             complement(12106..12810)
                     /gene="traK"
                     /locus_tag="RO454_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDY0935269.1"
                     /translation="MLGGLFFSCSVMAGVGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(12821..13387)
                     /gene="traE"
                     /locus_tag="RO454_003489"
     CDS             complement(12821..13387)
                     /gene="traE"
                     /locus_tag="RO454_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDY0935270.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETADASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13409..13720)
                     /gene="traL"
                     /locus_tag="RO454_003490"
     CDS             complement(13409..13720)
                     /gene="traL"
                     /locus_tag="RO454_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDY0935271.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(13735..14100)
                     /gene="traA"
                     /locus_tag="RO454_003491"
     CDS             complement(13735..14100)
                     /gene="traA"
                     /locus_tag="RO454_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDY0935272.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(14143..14310)
                     /locus_tag="RO454_003492"
     CDS             complement(14143..14310)
                     /locus_tag="RO454_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HDY0935273.1"
                     /translation="MLDETTNQKLLAARDRSGRTKTNEVFIRLKDHLNRFPDFYNASL
                     VKEEAESIDDI"
BASE COUNT         3308 a         4049 c         3455 g         3537 t
ORIGIN      
        1 atcctctggt ctgtggtaat caatctcact attggatttg gcatttatga caaattagca
       61 ccagtcattt cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca
      121 acataataca aataaacatc cttctgaaac tgtcaaactt cgtgataatc ggcatggtgc
      181 tgactgccat tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca
      241 agaaattatc tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc
      301 tcagtagata aactccttta ttatttagtc ctgtaaagaa ataaccaaca acccccacga
      361 taaacaggct ggcacctgta aaaaaacaca aatcccctgt atgcatatat atcctttggt
      421 taaaaattcg gtttaaaatc ctcagagaca ttcaggaact gttttgccag gtcgtcctgc
      481 gaaataaaac cgtatgataa cggacgaaca ctaccctgtt tcgggtcaac cagcatcatg
      541 gccgggaaat atttcacgcc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc
      601 aacagcggat taatcacgcc atccacggaa acgggaataa cactcagacc ataggtatcc
      661 cggaagccat taatgacctg cgccagctgc ccgtcgatgg ggtcctgccc ccggtaaaaa
      721 aacatgatgc cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct
      781 gctgccagct ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt
      841 tcaggatgtg ccagcatggc ctttctggcg ctcatggtga aaagcccggc ctgctgagtc
      901 cagtaatttt gcagccggaa atatttcaca aaattatcca cgccgggata cagaatggct
      961 tcgtacagcg cccgctttgt tgccgtctgc agtgcggcca gtttctgcat aatatccggt
     1021 tcctgacggg gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg
     1081 ttataccact gccagcctgc atcttttcct gacgccggaa aaataaagca gcagagtaac
     1141 agtggcagta atgctttatt catcttcatt cctttcatca ctttttcttt catcaggttt
     1201 ctgcaggtca gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta
     1261 aaggataacg aaagcagcgg caagcatccc gtctgccgtg gtttctttta ccgtatccca
     1321 cgaccagata attaccggag aaatgacaat cgctgtaatc aaaagccgga taagaaaatc
     1381 aataaaacgg ttcgacgtga atatgacttt catcatttcc cccggttatt tcccatacct
     1441 tactgtcctg cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg
     1501 ccactgtccg ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt
     1561 ctgttgaact gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc
     1621 gcactaccaa aactgatacg caactgaccg ttgcgcccct gctgctggac tatctgcgcc
     1681 agcttcgaat caaactgaca gtagctgcgt tttttctcca gacagacacc cagcactttc
     1741 ttcgaacaga actccccgac gctgacggtc agtttgtttg attttgcttt agccagggcc
     1801 ttttcatcac tgctgcattt ggccagcccg atatcctgtc cccagccgct gtccttacag
     1861 cagttgctgt atccggctgc agccttctta caaaatttcg cctgaccggt gaaagcacgg
     1921 acatccaccc cgttcagcgc cgcaacatcc ttacctgctg cggccagtgc cgccagctga
     1981 gaaacggctt cggcaaaatc attactctgc ccgctctgag ccttatcgca ttcgccatcg
     2041 agacagaaga cgtctccccc acagaccatc actttcccgg atgtttttga ctcacaggag
     2101 tacgtggcat attcatgcag acaggcacct tcttcggagt aaaaggcaca ctggtgtgac
     2161 accagggtac aggcaggatt gtcggtgtag gtctgacagg ttcccttgtc tgctgactgc
     2221 gtgacatacg tgtcccggta tgcccagcag ctctggtaca tgctgtatga ctgaccgtct
     2281 ttcaccagcg tcctgttccc gccagcctcc gtacattctg tttttgtcag tttccccagc
     2341 tccttgctga acgggcagct ttcagaccag acaacatacg gggtgtacga ggcttttttt
     2401 accctgacaa ccagacgcag cttcattgcc gtatgacgtt tgaattttgt attcccgacc
     2461 cggacggggt gagatgacgt aaaagcaaca ccggcagtca gtgttttctg tactggtgta
     2521 aatgaggcat ctgctgagta tttggcattc cagctcagtg gggttcccag tactgtaatt
     2581 gtcataggta cactcttctg caggtaagta cgactccagc tgtaatgcgc atcgacaatt
     2641 ttaccggtaa cgggagaggt aatggagaca acatactgac cattcacttc acgtgcaggt
     2701 agctgactca tttcatactc cagcgtccgg gtttcccatg tggtactccc ctgcagctcc
     2761 atacgggcag ttcgtgtgca gtactgctcc acctgtaaat ccctctcaca ggtgtaattc
     2821 gtgtattcac tccggctaat ctcctgcgca ctgcactgct gcccggtgtt tcccacaatg
     2881 ctgtcagccc ggttcaccac atccctgccg gtctggataa acggtgcatc cggtgaaaga
     2941 atgtctttcg gcttgttcat gaatgactct gtgatggttt ttccggtttc accggtcgcc
     3001 cattccgtgg tgccgtcatt tttcaggccg ccatccccgc cggcagtcac gccaccgtaa
     3061 tattttgtct cgtccggatt cgcgttatag ccggggatac tctcctgtgg cttaaagccc
     3121 tgaatactgc tgcttccctg ccctttgatc tggtgagcaa aatcagagcc ggcccggtaa
     3181 tcactgttgc tgtcagcctg tgccatgccg gcaaccagag ccagtatcag aggtaaaata
     3241 cgtttcattt cccggaatct cctttccctg ccagtaaatc atgagccacc tgccggcagt
     3301 cacccgtggc ggccactttc tccagcgcct ggccgacccg caggtttccc cggatgatgt
     3361 cgtatccctg actgcagaac accaccagta ccggtacact gcgaatgccg tactgtgaaa
     3421 acagcgtcgg gtcgatctga acaccatcag tcgcgccgtc tttcaccagg gacagcacgg
     3481 cttcagcggt ggtcttcagg tcattgttca ccatgccccg cagtgtggcg ggtataccgt
     3541 agtgccgggt ttcgcccagc atccgtttca gcccctcttc gggaatggaa aacgacacaa
     3601 aatacagggc accctgccgg ggtttcccgt cctgcgaagc ctgctgttta cgaaccactt
     3661 catccaggaa atggttatct gaacgctgaa gggggttttc cagcacctgt ttctccgccc
     3721 aggctttcag ctgatggtca ggtttttcac gcagttgtct gcttaaattt tcctgctgct
     3781 tcaggaactg gcggttttca ggagtgttca cgttttctga cgccataacc gccccgttca
     3841 gcatcatcag cagtgctgcc agagatttca tactcagctt catcattcac ctcacaggaa
     3901 tacacagtta cgtttacgcc acatcaggta gccgaagttt ttctttgtgt tgggtggatt
     3961 tttcccggtt tcccagcggg tcacgctgtg cccgaacggg tggcactgtc cgctgtccgg
     4021 atacatattc accatctggt aacgccagcg ttctttgggc aggattgggg acggatattc
     4081 attacagacg gcgttatttt tcccgatggt ttccataatc atgccctgac ggtgcagctt
     4141 gaacgccatg cgttcactga ccagcaggga ggactgcaac ggactggact cattactcac
     4201 ccagccattg aacgggtaca tacttccctg cgaaccggca caccagaaca gagcatcgat
     4261 gggcatgtta aaggcgctgg caatggcatc tgcagcacag gccccctgtg ctatcggatt
     4321 ggcaaaaatg acggcttccg gattgagaat ggtggtcagg ctgctgtccg tccaggtggg
     4381 gtcgatttca gaaagataag cgatatccag gtcaccacct tccagacagc ccagcgatgt
     4441 gatgatgtta agccagtacg tcagcgggta tttgtaccag tgaacgtgat agaacgcccc
     4501 gttcacctgc ttctcatctt ttttcgccgt tccctgtgcc gttttaccaa aagccggcag
     4561 gctgaagccc aggttaacca tgcatcccgg tgatcgggtg acgtccgtca gcgccatcgg
     4621 ctcccagtaa ccaatggcca gcccgatacg cctgaacagc ggcggcggtg ccggacaaat
     4681 ctgaatgggc atcgacgggt tcgccgtgtc ggggacctta ccctgactga ctttgatact
     4741 gcccagcgag agcgggaaaa tacagctcca gcagatatcc gtgatcgggt ttacaaaacg
     4801 cccctcacag gcagaatccg cagacgctgc agggacatga ccggcgagaa ggaataacat
     4861 cagcagccac aggcttcgct tcattttctg ccctcctctg ccggaataaa ttccaccttc
     4921 aggaagcgat cgccgggaac ggcgctcacc cgtgccggga cctgatcaat gccgaggcgc
     4981 tggcagagca caccgttctg gtcaaagtaa acacggctgt ccagagactt ctgcatctcc
     5041 gggatactgc cctgcacgag gataattttg ctctccagtg tgggcggcgt ctggcgtttc
     5101 atccaggcca cctgcgccgg atcatcgcca ttgatgaaat acagcgtctg gttaaaagga
     5161 acatactgca gcggattcat cacttcaccc tgacgggcaa acacccgccc ttcgttatcc
     5221 cggatatccg cagccagtct gacggacggg tcaaacaacc ggctgccgta tttctccgtg
     5281 cgtccgatac cgggcacggc agggggccgc agactgttgc ggatcacccg ctccttaaag
     5341 gcatccattt tccggcccat ctcaccggac tgctccagtg ccgtcaggcg ctgcatgatc
     5401 accgtcagca tgtccggctc ctttaccggc cacagatcgc cccaggtacc aagatcggcg
     5461 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggctccg
     5521 ccctcattcg ccgggcgata tcctgctgga tttcccgggt gatatccgga gccccctgta
     5581 ccacagcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
     5641 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgcgcttct gacagttgtt
     5701 tctgactggc gctgtcaaaa aacgcatcca cggtctgttt catgttgaag gcgacagtca
     5761 ccggtgcgtt cagtctgaca atgccgtaac tgacggctgc gttcagtaac accattgcca
     5821 ggcatcccgg caccgtccac caccagtggc tgcgtcgttc tgccgtgacg tcggcgggtt
     5881 tctgcgttga actcatgcca cactcctgta tttctcatgt tcttccagcc aggcttccag
     5941 cgaagccatc tccggccctg acttcttcca cgccagctgc cacaccgtct catgaatact
     6001 cagcccctct ttacgtttct gctgcacaaa ctcaaaatcc gggccgtcag agctgtacat
     6061 ggcgcggctt aacgggtcca caaacagacg atgccaggag gaatggtttt ccacctgcag
     6121 caggaaggaa ctgaaccact ggtctttggc cgcaccaaac ttaccaatca tgtcacgctg
     6181 cagtggcagg aactggtccg gatacagctg gttgtatttc gcgaactcct tcgcactctg
     6241 tttgaggata attttgtagg aggagttacc ccatgccgcg cgtgcagcac tggaggcctt
     6301 gtcagagtca aagtcgacga tgttctgtgt gatggtgata taggcaccgg tatgacggcg
     6361 ggcggtacgg taacctttct caataaattc accgaccttg tgatttttga agtccagcag
     6421 acgccagcct tcatcaataa cgttcagttt tttgagatta cgcggcgtgc ggtacatcct
     6481 gttctcgatg taaatgataa ggctgaacat caccgcaacc agcagtgacg gacggtcttc
     6541 cagtccgccc agctccagca ccaccatttt tgcgtcatcc cgcagggacg gctcatcaga
     6601 gttaaaatac tggccgtaag tgccgttggc agtgtactgg tcaagcagca caatcatttc
     6661 gtccagacgg ctgcggatgg tcggtgactc gacatactgg tcgttatcac gggcgttttt
     6721 caggaaatcc accacatcat cgatgcgcgc cttattcttt ttagccagcc aggaggccct
     6781 gaccgcctgc agcagcagac cttcatgtac ttcatccagg ttaccgttgg ggctggccat
     6841 caccgacaac tggtcacgga cacgctccgc tgactggtca atatcggtga tgttcgcaaa
     6901 cgggttaaaa cgcagggttt caccgtccag atacacccca cccatgttct cacacaggga
     6961 cttatagcca tcccccatgt cgaacaccac ggcaaagccc ccggagtcca gcacgctgcg
     7021 gataagtggc tgtatcagcc cggttttacc ggccccggag gtgccacaga ccgccatgtt
     7081 gtagttggtg ttattcatcc cccggaagaa aatatcgata aacgccagct ggttacggta
     7141 ggtgggtgcc agcagacctg ccggtgtcag ggggttatcc gccactaacg gcatcaggtt
     7201 ggcaacatta aagctctctg cgcgctgaac cacacctgcc tctttcagct gtttaaacag
     7261 ccctttcccg gccataaagg gcagacaggt caggaaattg cgcatgtggt taaagcgcgg
     7321 tgaaatcagc tcaaaaccgt ttttacgaaa gctgttcaga atgtcctgct ccacttccag
     7381 cgccgtttca ttgttgtcct tgcagaatgc tgtgatgttg aggaagtagg acacgacaga
     7441 agactgaccg gagcccagcc gctgacgcag ttccccccac tccttcgcct ctttctccac
     7501 ggacggaaac catttggcat aggaggtctt cgactttttc tccaggtcca tgtacttcag
     7561 gttggcttcg ctgtgggttt tcacctggtc ttccaccacc agagtcagcg tcaggatgaa
     7621 cggacaggag atggacagtt ccgggttcag caggttgctg tagttgtcgg ccatgttcca
     7681 caggaaggcg atttccgggt tacgggccag atggaaattc aggatgcggg ccgtgctgtt
     7741 cctacccttc tcacgcaggc ccagcgtcag gtaatccgcc cggactttca ggtcaaaact
     7801 gtcctccaca cactgataat tcagatcaga atacgggtcc agctgacgtc ttttcggata
     7861 cagggagtcc gggttatggt taatcatctc cccgacaata tcgataaagg cctgtgcatc
     7921 caccgcctgt gtggtgatac tggccccctg taacgacgcc cggatgattt tcaccaggtt
     7981 ttccatttcc agaatatcag cccggctttt tttcttcgag ggagaacagt acgagaagaa
     8041 tacccggtaa tggcgcaggg tcaggggcag attcatcccc tccggcagcg gaaactgtgt
     8101 cgctgccgcg ttcatataat aggcacgggt aatggcgtta aaccgttcag cctgttcacc
     8161 cgaccaggag aactcacgca gcccgtattc aatcctgtca ccaaccagct gactggacat
     8221 cagatggatg cagaacggca ccccgcgcgg cagtttggtg cgcagcatgt gatcgagggc
     8281 ctccacaatg gactcattcg ccccattgat gggaatggct tccagcataa agcccatcgt
     8341 ggtgtcattc atgaacagac cggattcctg gttgtaatca cggtacggca gcagacgact
     8401 gaactgcggg aagctcattt cgcccagaac ttcattggcc tttgcggatt cgtcaggcag
     8461 tttcagtgct gtgacgaggg agttaaccgc ctgagtgacg gcctcaagtg gattattcac
     8521 atgaattcct tagttgaaaa ataaagcgag aaatgcggta gccatgaagg gaatacctgt
     8581 aattaacgcg ccatttcccg gcgcatatac aaagatttct gttttctgga tatccactca
     8641 tgttcatccg gaattaaatc actatgttct gcgactgact tatgcataat gttttctctg
     8701 tctttcctga tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg
     8761 ggcttccgga acaggatttc cgcatgcttc acagagataa acaggaatac catgagcatt
     8821 tattttctgg cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc
     8881 ttcatcggct tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc
     8941 atcccttttc acgcacaggg cactcgtttt acaggcagat tttgaataca gaaagcctgc
     9001 cggcattgcc ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac
     9061 gcagacggtt taataacaaa aaacacgctg gatggctgat gataaacatc ctgattatca
     9121 atataaggcg ctatccataa tgcagccgtc tgctcacccg ttcttagcgg acgaggaggt
     9181 gttaccggcg caactgaact gaccggaaca atcgttttca cttcccgggc agcggtaaac
     9241 agcgggcgtg gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg
     9301 gacggagtgg tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg
     9361 cacggcagtg atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc
     9421 tcattggcct gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc
     9481 gtactggttc cggcacagcc ggaaagtaat aaggtcccca gcagaggaat aaagaaagaa
     9541 atctgtttca ttgtccctca cctttctcat cgcagttcac aaaatccttc ttcaaatccc
     9601 tgaccgtaca ggtttttaca atgtcaggat tttcaaaaac cgtactccgt gatgatttat
     9661 aaacatatgc cacaatgcgt ttcccacccg catcaggttc tggtgcactg acttcacata
     9721 acggaggtgt tattccggac tcatacagca ccggataatt tatttttttg acgctgccat
     9781 cagaaaccag cttattcatc gaagggctcc ttatatttct gaataattct gtcgattagt
     9841 tctttcaccg gaacagattt tccgccaccg gattgtccgg caaccacaat atgccccgga
     9901 gaagaaaata aactcatcga catacccttc ttcatcacac cgacagcagc catattaaaa
     9961 ttgtccggca atgactaaga accgaatttc tccggtgcag gaagtacgat tcttgtggcg
    10021 cagttactga gaacagcatg aataaaatca tctgacacgg ttttacccgg cacagagagg
    10081 gctgtaataa cattaatatt acgcatgttc atttttatct ccgttgtttc tgtttgttct
    10141 gtcatcagga agataaacag ggcatttcct gatttttccc ggcagggaag cgtcttccgt
    10201 ataaacttct ttcagatgtt catctgcggt taccacccgg atacatttca tttttcctgg
    10261 tcgcaccgga tatttcccgg tgatcgctgc cagcgagaca tacatcagtg ccagtgttgc
    10321 ccataatgcg gtgaccagtt gcatcagtga ccggtcagtc tccctgacat aatctgcagc
    10381 atcagccgcg cggtaatggc agatatcctg tatttctgat ctgcctgaag aatcagagga
    10441 agaaaaccag cattcactga ccgggaactg tccggcaggt gctgtttgtc ttacgaagtt
    10501 aatctcctta accagtaatt tccccggcgt ggtgttgtcc ttccataaaa caaacagagc
    10561 catcagggcc attgtggcca gtggcagaat gacggtgtac ttcagacacc agaacagtcc
    10621 tcgcagaacc cgggcgacaa cataccgggc ggcatggcac gcctgacggg acgacatatt
    10681 atttgccatc gttgccccct ccctggctgc cggacataaa ctgctgcgga ttcaggttgc
    10741 ccagctgctt cagcatctgg tcagtattaa agccgttaag atgactttca gccgatggcg
    10801 gaacaggaac cggactttcc ggattgtctt cttccgctct gctctgcgtg cgttccagcg
    10861 ccatctcttc cacggttttc agctggaagc cgtcctggaa caccacggtc acttcgttgc
    10921 ccgcaccaat cggtatcacc gggtgatact gttcggcacg tttgatgtag tagtcactga
    10981 gcgtctgtgc ggctttcgat gcgccgccac cgatacccat tctcaggaca tcaccagccc
    11041 cgtaagcggc tgtggcaccc agcccgacag ccggctggga ggcgcgctcc attccctgac
    11101 cgataccgtc aacaaatccc gcgccccatg cccagccgag gattttgccg ttacgcatca
    11161 ccactccgcc cttgataccg tttttacccc ggaagctgac atgcccctta atcggcatat
    11221 caatagtttt gccgtccttc aggcaactga tattgcgggt gcgtacaatg gcacgctcac
    11281 tggacacatc cccccaggct tccagaccca caaaacatcc cgttgcgtca tacgtcttgc
    11341 tgttcggcat ttccaccagg ccggtgatac gcagctgcat cggcaccgtg gattcattac
    11401 cggtgacaga ggcattggca tccgcccctt caatcagcat ggctttcgca aaacttcctg
    11461 acggaatgta cggcagtgat ggtccctgtt ttccctcatt acgggtaaac accttacgct
    11521 gtatccggtt aggcaccggc acagactggt acatcacctg tggtggtggc gtgacaccat
    11581 tcccgggata aaacgccgtc ggcggtggaa cagcaacgct gccctgcggc gggaaagata
    11641 cgggtgtgtt tcctggctgt ggttcgcctt ccgggccggg cggtgaggca ggcatctgcg
    11701 gtacaggctc accggtcgcc gtgacgggat tagcacccag ggcttttacc tgctctgcca
    11761 gggcggcatt gtcctgtccc aacttttcaa tacgacgctg gtcatcaccg cgctgtttgt
    11821 tcagaacatc cagctcacga cggatttcct catactgctt ctgcatctgc gctgccgtca
    11881 cctgcatctc ggtggtggca tgctgacgca ccttgtcatc aaaggtcgta tccaccacac
    11941 cggtcatatc cggcacaggc tcctgttcag ccacggtttc accgttaccg gacatgtcca
    12001 catcagacag atacagtgcc ccaccaatcg cggaggctgt accgacaacc acaatcccca
    12061 gccacaggta ctgcttgcgt ttcacaatgg tattgatact ggccatcagt tgccctcccc
    12121 gtcacggatg acatacacat ccatgcgcgc acctgccagt aactggctgg caggctggct
    12181 gaacatcacg gcacgggttc ccggctgcca gaagtcactt tcccggatat tcagggcaga
    12241 cagcgtttta ttttccacgg caaagcggac catcttcagg tgattccccg tccatacggc
    12301 atctgccact gaagacagcc ccgccggggc ctgcagggtt tcctttgtca ccgggacctg
    12361 ataccagcct gcgggtaatt ttccgccacg gacggcctgg ctgatggtca caagcaggga
    12421 ttcgtaaggc gtggacgttt cccacgcacc ggcttcttct ccggttccgc gcaggtcact
    12481 gaccagctga atggtacgcc ccgcgccttc acggggaacg gcctgaatgg aaagattcag
    12541 accacgttct gtttccagaa tgaacgtaaa gggctttttg ttgacggtgg ccaccaccac
    12601 accgccggag gcggtctgct cattattggt cagggcacca tccagactgt tcacggcgat
    12661 aatccggtca ccgggaacgg taaacagatt cgggctggta ttgctgatgg caatactggc
    12721 ctgaccaccg ttcaccattg gcaccacggt gggggccagc gtaccgactc cggccatcac
    12781 gctgcaggaa aaaaacaggc cgcctaatat tattgctgta ttattttttc tcatcgtctg
    12841 tttccccgaa attatccagc caggtcaccc cgttttcccg cttcagaata aggatgtaat
    12901 gtttgatatc agtgaaaggt tttgaatcac caatccaggt tttaagcaca ccacgaattt
    12961 ccacacggcc atactgaggc cagacacgaa cactggtctg gaaaaaggct gagttcacgt
    13021 tatcgttttt aatacgcttc gcttcttcag ccagaataac tttcatctgg ttctgtgcgc
    13081 ccgggcggat atattgcaga agcgcctgat gtgaggcatc ggcggtttct gatgaaacat
    13141 tcagacggag ggcaataaat gacagcgcca tctgctgtaa ataagaggcg tcggcactgt
    13201 tctgtgacac ggcaaagggg gcattaaatg ccattggcgt cacggcagtg cgttgctcat
    13261 tctgaagacg gtagttattc accccctgaa tgacgttaac agagaggctg agaacaataa
    13321 gcactgacat aaatataaag gcgatggcca ttacacgact ggtacttaaa cgggcaccgt
    13381 gttccatata acaatcctgg tatcagttct atttaatcca ctgccggaaa cacgaatcgg
    13441 gaacattatg aaaaataccg cgcagcaggg ctgttggcat ataccagtaa atcaggtcac
    13501 gtaaccagga actgccccgc ccttttttca gttttttaat cccgaaataa accagaaccg
    13561 ctgcaccaat accgaacaga tatttcgatg ttgtgatacc ccagccaata cagattgctg
    13621 cggggatcag ttcatccagt ggcaggccaa accagcggct ctggttggtc agtgtttccg
    13681 ggaaacgata tttcttaagt ttattctcgt ctcccgacat cgttttattt cctgtcagag
    13741 gccaacgacg gccataccca cagcaataaa tacagagatg atggcaaaac cggccaggaa
    13801 cttgacgttt ttggtcatca tgtacatgac cgcaccgacc agaacttcag ccagaacaac
    13861 ccatttaaca acactggagt ccttaccgaa ggtcgcctta accgtggtgt taccacttgc
    13921 catcaggtcc tgaccactgc tgccggcggc catcgccagc tgcgggaaga acatcatcag
    13981 aacagcagcc gggatcacag cgcgagccag gcgaagcata ttcagacgag tgaatttaga
    14041 aaaaaacgac ttctttttga cgggcgcaga aaccccctga acacttaaaa cggcgtccat
    14101 atgattcctc tgtttaatat tgatagtgaa aaataaaact ctttatatat cgtctatgct
    14161 ttcagcttcc tcctttacta aagatgcatt ataaaagtct ggaaaacgat tgagatgatc
    14221 ttttagtcta atgaacacct cattagtttt tgttcttccg ctacgatctc tagctgctaa
    14281 aagtttctga ttagttgtct catccagtat taataagact ttgttacccg gtgcggctct
    14341 ggttctccc
//
LOCUS       DAPQZD010000119        14275 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPQZD010000119 DAPQZD010000000
VERSION     DAPQZD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.121"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            220..1119
                     /gene="lysO"
                     /locus_tag="RO454_003493"
     CDS             220..1119
                     /gene="lysO"
                     /locus_tag="RO454_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDY0935274.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1263..2915
                     /gene="hcp"
                     /locus_tag="RO454_003494"
                     /gene_synonym="priS"
     CDS             1263..2915
                     /gene="hcp"
                     /locus_tag="RO454_003494"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDY0935275.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2927..3895
                     /gene="hcr"
                     /locus_tag="RO454_003495"
     CDS             2927..3895
                     /gene="hcr"
                     /locus_tag="RO454_003495"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDY0935276.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            4028..5746
                     /gene="poxB"
                     /locus_tag="RO454_003496"
     CDS             4028..5746
                     /gene="poxB"
                     /locus_tag="RO454_003496"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDY0935277.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5783..6784
                     /gene="ltaE"
                     /locus_tag="RO454_003497"
     CDS             5783..6784
                     /gene="ltaE"
                     /locus_tag="RO454_003497"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDY0935278.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6795..8225
                     /locus_tag="RO454_003498"
     CDS             6795..8225
                     /locus_tag="RO454_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDY0935279.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8324..9337
                     /locus_tag="RO454_003499"
     CDS             8324..9337
                     /locus_tag="RO454_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDY0935280.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9334..10164)
                     /gene="amiD"
                     /locus_tag="RO454_003500"
     CDS             complement(9334..10164)
                     /gene="amiD"
                     /locus_tag="RO454_003500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDY0935281.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10161..10484)
                     /locus_tag="RO454_003501"
     CDS             complement(10161..10484)
                     /locus_tag="RO454_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDY0935282.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10610..11125
                     /locus_tag="RO454_003502"
     CDS             10610..11125
                     /locus_tag="RO454_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDY0935283.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11343..12071
                     /gene="artP"
                     /locus_tag="RO454_003503"
     CDS             11343..12071
                     /gene="artP"
                     /locus_tag="RO454_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDY0935284.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12089..12820
                     /gene="artJ"
                     /locus_tag="RO454_003504"
     CDS             12089..12820
                     /gene="artJ"
                     /locus_tag="RO454_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0935285.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            12827..13543
                     /gene="artQ"
                     /locus_tag="RO454_003505"
     CDS             12827..13543
                     /gene="artQ"
                     /locus_tag="RO454_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDY0935286.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13543..14211
                     /gene="artM"
                     /locus_tag="RO454_003506"
     CDS             13543..14211
                     /gene="artM"
                     /locus_tag="RO454_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDY0935287.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
BASE COUNT         3256 a         3750 c         3795 g         3474 t
ORIGIN      
        1 ccctcgactt atcccttacg gctaccgggt cggcgatttt gacgccacca acaccggtgc
       61 ggtttgtaat taaagcgcaa gaatatcttt aaatgaaaat gaatacccta ccgatcctta
      121 tgcataacat ttccttttaa ttgcaacttt actcgtcctt ccgctttcag tagtgtgtca
      181 tcgcgcattt cgaatcttct ttgcaaggac tgggttttca tgttttctgg gctgttaatc
      241 attctggttc ccctgattgt gggttacctc attccgcttc gccaaaaagc tgcattaaga
      301 gttattaatc agctcttaag ctggatggtt taccttattc tcttttttat gggtatcagt
      361 ctggcgtttc tcgataacct cgccagtaac ttgttggcaa ttctgcatta ttctgccgtc
      421 agtactaccg ttattttact gtgtaatatt gccgccctga tgtggctgga gcgcggcctg
      481 ccatggagca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat ggcgctggag
      541 tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag tggactggct
      601 ttcttacaac acgcgaccga agccagtgaa tacaccttaa ttctgctgct tttcctcgtc
      661 ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa tcgccgggga
      721 atgattgtcg ccgtggtggt ggttgccagt tcattaattg gtggtttaat taacgccttt
      781 attcttgatc ttcccatcaa taccgcgctg gcaatggcct ccggtttcgg ctggtattct
      841 ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc ggcgtttttt
      901 aatgatctgg cccgtgagct gattgctatt atgttaatcc ctgggctgat tcgccgtagc
      961 cgctctactg cactgggctt atgcggtgcg acgtcgatgg atttcaccct gcccgttctt
     1021 caacgtacag gcgggctgga tatggtcccg gcggcgattg ttcacggttt tattcttagt
     1081 ctgttagtgc cgattctcat cgcctttttc tctgcataat acctctctgg cggtagatct
     1141 ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcattaaaaa tctcttttat
     1201 ccccgcgtta agcgtcttaa ccttaaacat gtatattaaa tataacttta aaaggtgtga
     1261 tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcaggaaac ggctgctcat
     1321 acgcgcaggg gatgtgtggt aaaacggcgg aaacctctga ccttcaggat ttactcatcg
     1381 cgacgctgca agggctttcg gcctgggcag tgaaagcacg tgaatacggc atcatcaacc
     1441 acgatgtaga cagctttgcg ccacgtgcct ttttctcaac cctgaccaac gttaacttcg
     1501 attctccgcg tattatcggc tacgctcgtg aagcgattgc cctgcgcgag gcgctgaaag
     1561 cacaatgcct ggctgtagat gccaacgccc gcgtcgataa cccgatggct gacctgcaac
     1621 tggtgagcga cgatctcggc gagctgcaac gtcaggcagc agaatttacc cctaacaaag
     1681 ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg tatggcctga
     1741 aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac aacgatattt
     1801 atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat atgaacgcgc
     1861 ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc attctggacg
     1921 caggcgaaac cggtaaatac ggtcacccga ccccaaccca ggtcaatgtc aaagcgacgg
     1981 caggtaaatg catcctgatt tccggtcacg atctcaaaga tctctacaac ctgctggaac
     2041 agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct gcgcatggtt
     2101 acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc tggcagaatc
     2161 agcaagtgga gttcgctcgt ttcccaggcc ccatcgtgat gacctcgaac tgcatcatcg
     2221 acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt ggctggccgg
     2281 gtgtgcgcca tctggatggc gaagatttct ctgcggtcat tgcccaggcg caacagatgg
     2341 cgggcttccc gtacagcgaa attccgcacc tgatcaccgt gggctttggt cgccagacac
     2401 tgcttggcgc agccgatacg ctgattgatc tggtgagccg tgaaaaactg cgtcatatct
     2461 tcctgcttgg cggctgtgac ggcgcacgcg gcgagcgtca ctacttcacc gatttcgcca
     2521 ccagcgtgcc ggatgactgc ctgatcctga ccctcgcttg cggtaaatat cgctttaata
     2581 aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgctggt cagtgtaacg
     2641 atgcttactc agcaattatt ctggcggtaa cgctggcaga gaaactgggc tgcggagtca
     2701 acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt gtcattctgc
     2761 tgacgctgct ttctctgggt gtgaaaaata tcgtcaccgg cccgactgca cctggtttcc
     2821 tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct atcaccactg
     2881 ttgaagaaga catgaagcaa ctgttgagcg cgtaaggagt ttgtcgatga cgatgccaac
     2941 gaatcaatgc ccgtggcgga tgcaggttca tcacattacg caagaaacgc cggatgtgtg
     3001 gacgatttcc ctgatttgcc acgattacta cccgtatcgc gccgggcaat atgcgctggt
     3061 cagcgtgcga aactcagcag aaacgctgcg tgcttacacc atttcctcca cgccaggcgt
     3121 gagtgaatac atcaccctga ctgtgcggcg gattgatgac ggtgtcggct cccggtggct
     3181 gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg gggaatttac
     3241 ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg gcgtcacgcc
     3301 gattatgtcg atgcgtcgct ggctggcgaa gaaccgtcca caggccgatg tgcaggtgat
     3361 ctacaacgtg cgtacgccgc aggatgtgat tttcgccgat gagtggcgta actatccggt
     3421 aacgctggtg gcggaaaata acgttaccga aggctttatc gctggtcgtc tcactcgcga
     3481 actgctggca ggtgtccctg atttagcctc acgtaccgtg atgacctgtg gccctgctcc
     3541 atatatggat tgggtagagc aggaagtgaa aacgctcggc gtgacgcgtt tctttaaaga
     3601 gaaattcttc accccagtag cggaagcggc gaccagcggt ctgaaattca ccaaactgca
     3661 accggcacga gaattttacg ctccggttgg caccacgcta ctggaggcgc tggaaagcaa
     3721 taacgttccg gttgtcgccg cctgccgtgc gggtgtttgc ggctgctgta agacgaaagt
     3781 gatttccggt gaatatacgg tgagcagcac aatgacgctg accgacgccg aaatcgctga
     3841 aggttacgta ctggcctgct catgccatcc gcagggggat ttggttttcg cataatcgcc
     3901 tgatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc ctttttctcc
     3961 catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat tcaggagatg
     4021 gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat cggcaggcgt
     4081 gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagtgaca gtcttaatcg
     4141 catgggcacc atcgagtgga tgtccacccg gcatgaagaa gtggcggcat ttgccgctgg
     4201 cgctgaagca caacttagcg gagaactggc ggtctgtgcc ggatcgtgcg gtcccggcaa
     4261 cctgcactta atcaacggcc tgttcgattg ccaccgcaat cacgttccgg tactggcgat
     4321 tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa cccacccaca
     4381 agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccag agcagatccc
     4441 acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt ccgttgttgt
     4501 gttaccgggc gatgtggcgt taaaacctgc gccagaaggg gcaactaccc actggtatca
     4561 tgcgccacag ccggtagtaa caccggaaga agaagagtta cgcaaactgg cgcaactgct
     4621 gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgtgcggggg cgcataaaga
     4681 gttagttgag tttgccggga aaattaaagc gcctatagtc catgccctgc gcggtaaaga
     4741 acatgtcgaa tacgataatc catatgatgt tggaatgacg ggattaatcg gcttttcgtc
     4801 aggtttccat accatgatga acgccgatac gttagtgctg ctcggtactc agtttcccta
     4861 ccgtgtgttc tacccgaccg atgccaaaat tattcagatt gatatcaacc cagccagcat
     4921 cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa ccctgcgcgc
     4981 attgctgcca ctggtggaag aaaaaaccga acgcaagttt ctggataagg cgctggaaga
     5041 ttaccgcgac gcccgcaaag ggctggacga tttagctaaa ccgagcgaga aagccattca
     5101 cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcta ttttcacctg
     5161 tgacgttggt acgccaacgg tgtgggcggc acgttatcta aaaatgaacg gcaagcgtcg
     5221 cctgttaggt tcgtttaacc atggttcgat ggctaacgcc atgccgcagg cgctgggcgc
     5281 gcaggcaacc gagccagaac gccaggtggt cgccatgtgc ggcgatggcg gttttagcat
     5341 gttgatgggc gatttcctct cggtagtgca aatgaagctg ccagtgaaaa ttatcgtctt
     5401 taataacagc gtgctgggct ttgtggcgat ggagatgaaa gctggtggct atttgactga
     5461 cggcaccgaa ctacacgaca ccaactttgc ccgaattgcc gaagcctgcg gcattacggg
     5521 tatccgcgta gaaaaagcgt ctgaaatcga tgaagccctg caacgcgcct tctccatcga
     5581 cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc caccgcagat
     5641 caaactcgaa caggccaaag gtttcagcct gtatatgctg cgcgcaatca tcagcgggcg
     5701 cggtgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag ggtggcattt
     5761 cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta cccgaccgag
     5821 ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt atggagacga
     5881 ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag aagccgccat
     5941 ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact gcgaacgcgg
     6001 cgaagagtat atcgtcggtc aggccgcgca taactatctg tttgaagccg gtggcgcagc
     6061 agtactgggc agtattcagc cgcaacccat cgacgcagct gccgacggta cgctaccgct
     6121 ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca ccaaattact
     6181 cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga aagaagcatg
     6241 ggaatttacc cgcgagcgca atctggcgct gcacgttgac ggtgcgcgca tttttaatgc
     6301 cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgact cgttcaccat
     6361 ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta atcgtgatta
     6421 cattaaacgt gccattcgct ggcggaaaat gacaggtggc gggatgcgtc agtccggcat
     6481 tctggctgcc gccgggatgt atgcgctgaa aaataacgtc gcacgtttgc aggaagacca
     6541 cgataacgcc gcctggatgg cggagcagtt gcgtgaagca ggcgcggatg tgatgcgtca
     6601 ggacaccaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt taggcgaata
     6661 catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg tgacccatct
     6721 tgacgtctcg cgtgaacaac tggcggaagt tgccgcccac tggcgtgcgt tcctggcgcg
     6781 ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc tacattggtc
     6841 aacatctggt gcacacactc agccagcaag ggcatcagat cctggcggcg gcacgtcacg
     6901 tcgacagact tgcaaaactg caactggcta atgtcagttg ccataaagtc gatctcaact
     6961 ggccggataa ccttccggtc ctgttgcaga atattgatac tgtctatttt ctggtgcaca
     7021 gcatgggcga aggtggtgat tttatcgctc aggagcgcca ggtggctctc aacgtccgcg
     7081 atgcgctgcg cgaagtaccg gtaaagcaag tcatctttct cagttcattg caggcaccgc
     7141 ctcatgaaca atctgatcat ctgcgtgctc gtcaggccac ggcggatatt ttacgtgaag
     7201 cgggtgtccc ggtgaccgaa ctacgtgccg gaattatcgt tggcgcgggt tcagcggcat
     7261 tcgaagtcat gcgcgatatg gtttacaacc tgccagtatt aacgccacca cgctgggtac
     7321 gttcacgcac tacgcccatc gcgctggaaa acctgctgca ctatcttgtg gcgctgttag
     7381 atcatccggc ttgcgaacat cgcatcttcg aagccgctgg gccagaagtg ctcagttatc
     7441 agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg ctccccatcc
     7501 ccctccccac ccgctggatt tcggtgtggt ttcttaatgt gattacttca gtaccgccca
     7561 ccaccgccag agcgttgatt caggggctga aacacgatct gctggcggat gacaccgcgt
     7621 tacgtgcact cattccacaa ccgctgatcg ctttcgatga cgcggtacgt agcacgttga
     7681 aagaggaaga aaaactggtc aactccagcg actggggata tgacgcgcag gcttttgctc
     7741 gctggcgacc agaatacggt tattttgcca aacaggcggg gtttaccgtt aaaacgtctg
     7801 ccagccttca ggcgctatgg cgggtggtca atcaaatcgg cggtaaagag cgttatttct
     7861 ttggcaatat tttgtggcag acacgggcgt tgatggacct tgcgatcggt cataagctgg
     7921 caaaaggccg cccggagcgc gaatatttac agaccggcga tgctgtggat agctggaaag
     7981 tcattgtcgt tgaaccggaa aaacaactta cgttgttatt tggcatgaaa gcgccggggc
     8041 tgggacgact ctgttttacc ctggaagata aaggcgacta tcgtacaatc gatgtccgtg
     8101 ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg atccccgcgc
     8161 atctgtttat ttttcgcgga atggcaaaac gaatcgccag actggcagaa caaagcacag
     8221 attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcaag ggggaaatca
     8281 cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg tactggttac
     8341 cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga aaggcatcag
     8401 cgtgcgagcg accggtcgca acgaggcaat gggtaaattg ctggaaaaaa tgggcgcaga
     8461 gtttgttccg gcggatctga ccgagttggt ctcatcacaa gctaaagtga tgctcgcggg
     8521 cattgatacg ctgtggcact gctccagctt tacctcgccc tgggggacac aacaggcttt
     8581 cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg cctggggtgt
     8641 gcgtaacttt attcatatct cttctccctc cctgtacttc gattatcacc accatcgcga
     8701 tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca gcaaagcagc
     8761 cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct ttactattct
     8821 gcgcccacaa agtctgttcg gaccgcacga taaagtcttt atccctcgtc tggcgcatat
     8881 gatgcatcac tacggcagta ttctgttacc gcatggcggc agtgcgctgg tcgatatgac
     8941 ctactatgaa aatgccgtgc acgccatgtg gctggcaagc caggaagcgt gcgataagct
     9001 accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc gcagcatcgt
     9061 gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttcggttc cttacccgat
     9121 gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa aagagccgcc
     9181 gctgacccac tacggcgtct ccaagttgaa ttttgacttt acgctggata ttacgcgtgc
     9241 gcaggaagag ttaggttatc agccagtcct caccctggat gaaggtatcg agaaaactgc
     9301 cgcctggctg cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt tctccagcaa
     9361 cgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg tcgggcggaa
     9421 atgcatctgg aacgccataa tcacacgccg ctgctcgcgc ggagtcatat caggctttac
     9481 gtcataacca taacgcgcca aaagctccag caatgacgcg gtctctaccg gtgtgtgcgg
     9541 cgcgcgcccg gcaaggtaaa agttcacccg ctgcgcatcc ggccaggcac caatcccctg
     9601 ctgtgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct gtggcgcaat
     9661 atccgcatgt gctactacgt tttccggctt gatgtgataa cgggcaataa tatctttcgc
     9721 cagcggaata agcgcctgaa tctgtgccgg ttcaaacggg gcaaaatatt tcactcccgc
     9781 cgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt taagacgcgt
     9841 tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg gcaccagttg
     9901 ccagatgcgc ggtttaccgt tgtatcgcgg tggcaccgca gggaccagat aatgcgagct
     9961 gacctgctta tcggtcagtg tcgccagcga gctatcaaaa tcatctgcgg tgtagtggat
    10021 caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg cgtcgggtat caagctgata
    10081 tccctctttc tcgacaatgc ctttttcgcc tgtgcaccct gccagtaaca gagcggcagc
    10141 gaccagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct gacgctaacc
    10201 atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc gacgacggca
    10261 tcagccccca gcgctcgcgc ctgggagccg agctcctcaa aggcaatctc ccgcgcttta
    10321 cgcagttctt tttcgtaggc accggagcgt ccgccaacga tatcgcggat accggcaaag
    10381 aaatcacgga aaatattcgc gcctaaaatc gcttcgccag tcaccacacc gcaatattca
    10441 acgatggtct ggccttccag agttggggtt gtagaaaatt gcatgatcga tctccttgtt
    10501 tgctaagcct tcgatcttca aagcattatc ggactgatac gctattattg aaaggatatc
    10561 attattatcg tagaacgctt tcaaggtgtt cgcttataag gaaatcatca tgcgctatag
    10621 caaattgacc atgcttatcc cttgcgcact gctactcagt gcctgtacca ccgtcacgcc
    10681 agcttataaa gataacggcc cccgcaccgg ttcctgtgtc cagggggggc ctgatagtgt
    10741 ggctcaacag ttctatgact accgcattca acatcgcagc aatgacatta ccgctctgcg
    10801 cccctatttg agtgacaaac tggcaaccct acttagcgat gccagccgcg ataatagtca
    10861 tcgtgaattg ttaagcagcg atccgttctc cagtcgaacc accttgccag acagcgccca
    10921 cgtagccagt gcatcgacta tccctaatcg tgatgcccgt aacattccat tgcgcgtgga
    10981 tctcaagcag ggtgaccagg gctggcagga tgaagtgtta atgattcagg aaggccagtg
    11041 ttgggtcatc gacgatgtgc gctacttagg cggtagcgtt catgccacgg cgggtacgct
    11101 ccgtcaatcc atcgagaatc gctaacgtct tgatcaacca ataaaccccg taaattgtcc
    11161 ggcatttcgt gaggaatgcc gacatttatg ctcgccgacc accgcccccg ttattttgtg
    11221 ctatgtttat tgaataatgc gctttgcttt taacttttaa agcagaaata ttgcataatt
    11281 attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt atacgagtgt
    11341 caatgagtat tcaattaaac ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg
    11401 atatcacgct ggattgcccg cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg
    11461 gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccgcgctcc ggtacgctca
    11521 acattgcagg caaccatttc gatttcacca aaaccccctc tgacaaagcg attcgcgatt
    11581 tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc
    11641 agcaaaacct gattgaagcg ccctgccgtg tactggggct gagtaaagat caggcgctgg
    11701 cccgtgcaga aaaactgctg gaacgtctgc gtcttaaacc ttatagcgat cgttacccgc
    11761 tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg atgatggaac
    11821 cgcaggtact gctgttcgat gaaccgaccg ccgcactgga ccccgaaatt acggcacaaa
    11881 tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc gtcacccacg
    11941 aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaac ggtcatatcg
    12001 tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt aaaaactatc
    12061 tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg cgttaattgc
    12121 aggttttagt ctttccgcca cagcagccga aaccattcgt tttgccaccg aagcctccta
    12181 tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgatg tcgacctggc
    12241 gcaggcattg tgtaaagaga ttgatgcaac ctgcactttc tctaaccagg cgtttgacag
    12301 cctgatccca agcctgaaat tccgtcgcgt agaagcagtg atggcgggca tggatatcac
    12361 tccggagcgt gaaaagcagg tgctgtttac caccccgtac tatgacaact ctgccctgtt
    12421 tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa aagttggcgt
    12481 tcagaacggg acgacacacc agaagttcat tatggataag catccggaaa tcactaccgt
    12541 gccatatgac agttaccaga acgcaaaact ggatctgcaa aacggtcgta tcgacagcgt
    12601 atttggtgac accgcagtgg taactgaatg gctgaaagat aacccgaaac tggcggcagt
    12661 gggcgataaa gtgaccgata aagattactt cggtactggc ctcggcatcg cagtacgcca
    12721 gggcaacact gaactgcagc agaaattcaa cactgcgctg gaaaaagtga agaaagatgg
    12781 cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga atgaattttt
    12841 tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg
    12901 gctggcgctg gcgatgttct ttgcggtatg ggagtctgcg aaatggcgtc ctgtcgcgtg
    12961 ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg tggtgctgtt
    13021 tatctatttt ggctcctcgc agctgctgct gacgctttcg gatggcttca ctatcaatct
    13081 tgggttcgtg cagatcccgg tgcagatgga cattgagaac ttcgacgtta gcccgttcct
    13141 ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa cgctgcgtgg
    13201 tgcattaaaa gcggtgccgg tggggcagtg ggaatccggt caggcgctgg ggctgtcgaa
    13261 atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc tgccgggtct
    13321 cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa
    13381 tgatttaatg ctacaaacca aaagcatcgc tacccgtacc caggaaccgt ttacctggta
    13441 cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca ttctcaaacg
    13501 cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga gtatttaccc
    13561 gaactgatga aagggctgca caccagcctg acgctaaccg ttgcctcgct gattgtggca
    13621 ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt gctggtgtgg
    13681 ctggtacggg gctatatcac gctgtttacc ggtacgccgc tgctggtgca gatcttcctg
    13741 atttattacg ggccgggcca gttcccgacg ttgcaggagt atccggcact gtggcatttg
    13801 ttgtcagaac cgtggttatg tgcgctgatt gcgttgtcgc tgaacagtgc ggcgtacacc
    13861 acgcagctgt tttacggtgc gattcgcgcg atcccggaag gtcaatggca gtcctgtagc
    13921 gccctgggaa tgagcaaaaa agatacgctg gcgatcctgc tgccgtacgc gtttaaacgc
    13981 tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc tctggcttac
    14041 accattacgc tcatggaagt gatgggatac agccagttgt tgtacggacg cacctacgat
    14101 gtaatggtat ttggcgcggc ggggattatt tacctggtcg tcaacggcct gctgacgctg
    14161 atgatgcgtc tgatcgagcg taaagcactg gcattcgaac ggcggaatta atgtagctgc
    14221 atgctgatgc cggatgcggc gtggacgcct tatccggcct acatagtgca taaat
//
LOCUS       DAPQZD010000120        14196 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPQZD010000120 DAPQZD010000000
VERSION     DAPQZD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.122"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(1..769)
                     /locus_tag="RO454_003507"
                     /pseudo
     CDS             complement(1..769)
                     /locus_tag="RO454_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            1293..3221
                     /gene="thrS"
                     /locus_tag="RO454_003508"
     CDS             1293..3221
                     /gene="thrS"
                     /locus_tag="RO454_003508"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDY0935288.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3225..3767
                     /gene="infC"
                     /locus_tag="RO454_003509"
     CDS             3225..3767
                     /gene="infC"
                     /locus_tag="RO454_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDY0935289.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3864..4061
                     /gene="rpmI"
                     /locus_tag="RO454_003510"
     CDS             3864..4061
                     /gene="rpmI"
                     /locus_tag="RO454_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDY0935290.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4114..4470
                     /gene="rplT"
                     /locus_tag="RO454_003511"
     CDS             4114..4470
                     /gene="rplT"
                     /locus_tag="RO454_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDY0935291.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4587..4711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4593..4637
                     /gene="pheM"
                     /locus_tag="RO454_003512"
     CDS             4593..4637
                     /gene="pheM"
                     /locus_tag="RO454_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDY0935292.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4921..5904
                     /gene="pheS"
                     /locus_tag="RO454_003513"
     CDS             4921..5904
                     /gene="pheS"
                     /locus_tag="RO454_003513"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDY0935293.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5919..8306
                     /gene="pheT"
                     /locus_tag="RO454_003514"
     CDS             5919..8306
                     /gene="pheT"
                     /locus_tag="RO454_003514"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDY0935294.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8311..8610
                     /gene="ihfA"
                     /locus_tag="RO454_003515"
     CDS             8311..8610
                     /gene="ihfA"
                     /locus_tag="RO454_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDY0935295.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8711..9691
                     /gene="btuC"
                     /locus_tag="RO454_003516"
     CDS             8711..9691
                     /gene="btuC"
                     /locus_tag="RO454_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDY0935296.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9754..10305
                     /gene="btuE"
                     /locus_tag="RO454_003517"
     CDS             9754..10305
                     /gene="btuE"
                     /locus_tag="RO454_003517"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDY0935297.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10305..11054
                     /gene="btuD"
                     /locus_tag="RO454_003518"
     CDS             10305..11054
                     /gene="btuD"
                     /locus_tag="RO454_003518"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDY0935298.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11132..11596
                     /locus_tag="RO454_003519"
     CDS             11132..11596
                     /locus_tag="RO454_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDY0935299.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11844..12557
                     /gene="ydiV"
                     /locus_tag="RO454_003520"
     CDS             11844..12557
                     /gene="ydiV"
                     /locus_tag="RO454_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDY0935300.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12620..14056
                     /locus_tag="RO454_003521"
     CDS             12620..14056
                     /locus_tag="RO454_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDY0935301.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(14060..>14196)
                     /gene="hemP"
                     /locus_tag="RO454_003522"
     CDS             complement(14060..>14196)
                     /gene="hemP"
                     /locus_tag="RO454_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDY0935302.1"
                     /translation="ASPQPIRRITSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK
                     "
BASE COUNT         3580 a         3271 c         3776 g         3569 t
ORIGIN      
        1 ctatatataa aatcgcggac atatgactga cattgtttgc catcattcga cagaagaaaa
       61 tatctaaagc aaagccaaaa tcctgactcg cgataacgcg actccttgca agaggatggc
      121 gaagtaatgc tctgaattcc gcgtcccgga ctaagggaac atgctgctgt atggattcaa
      181 tggaggagta atgtttaatc aggttgactt tgccttttcc atttctcaat gaatcaaggg
      241 cgtattgtaa tgacggatgg cgacaaaaaa tagtgtcaga aggagattgc gaaaaaatac
      301 actatcattg agatttactg ttggctgtat ggttttttct ctatgaattt ttttggacag
      361 tttcatgcaa tggttacgtt tttcggcagt taactgttcc cgttcaatat aatccagtat
      421 gcttcgcaaa tggttagctt taataggcgt ggtttccaga aataaggaaa tgatttattt
      481 tttatagtgt aatgagtgta ttttctgcat ggatgggtga agtggcaaag ttaatgataa
      541 aaagggcagt ggaataacaa ttgtgtgata tttttcgaca gcttacggta aattcctgag
      601 taacacagta tgacagcatc gaacttaaat cagaggtatc aagtagggtg atgagctggc
      661 gcgcaaattg actaataggc tctgatgaag gcgttagtat gtcgctatcg atgtgcattc
      721 taagaggaga ctcataatga gttctgataa tgatatcgtt ttcactcata atctattcgc
      781 cttatatatg tctggagatt ttgaggatga tgattaatta ttatcgccag aacgaaatgc
      841 gcacgaaaaa ttgctataag aattgtgtca cttttatcat tgcgatatga ccacttcgtc
      901 actgaatctc atgatcgttt aaataccacc gccaaataac gcaacgaact ttgctggatt
      961 cagtttatgc tgctgtaaat ccgctcgtag taaacctttc agacactctg tgatgatatc
     1021 agtggttctt taagcgtttt gctggtgtac tcactacaaa cgaattgcga atcgaatcaa
     1081 tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta acatcgttca accgggtgga
     1141 aggtgagagg ttaatgtctt tcatttgtgt acctgattgc gaaccaattt agcatttgtt
     1201 ggctagatgg tttcgcaatg aactgttaat aaacaaattt ttctttgtat gtgatctttc
     1261 gtgtgggtca ccactgcaaa taaggatata taatgcctgt tataactctt cctgatggca
     1321 gccaacgcca ttacgaccac gctgtaagcc ccatggatgt tgcgctggac attggtccag
     1381 gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga actggttgat gcttgcgatc
     1441 tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa ggtctggaga
     1501 tcattcgtca ctcctgtgcg cacctgttag ggcacgcgat taaacaactt tggccgcata
     1561 ccaaaatggc aatcggcccg gttattgaca acggttttta ttacgacgtt gatcttgacc
     1621 gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga
     1681 aaaactacga cgtcattaag aagaaagtca gctggcatga agcgcgtgaa actttcgcca
     1741 accgtgggga gagctacaaa gtctccattc ttgacgaaaa catcgcccat gatgacaagc
     1801 caggtctgta cttccatgaa gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca
     1861 tgcgtttctg ccatcatttc aaactaatga aaacggcagg ggcttactgg cgtggcgaca
     1921 gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa aaagcgctta
     1981 acgcttacct gcaacgcctg gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac
     2041 agctcgacct gtaccatatg caggaagaag cgccgggtat ggtattctgg cacaacgacg
     2101 gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa gagtaccagt
     2161 atcaggaagt taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa accggtcact
     2221 gggacaacta caaagatgca atgttcacca cgtcttctga gaaccgtgaa tactgcatta
     2281 agccgatgaa ctgcccgggg cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg
     2341 atctgccgct gcgtatggcc gagtttggta gctgccaccg taacgagccg tcaggttcgc
     2401 tgcatggcct gatgcgcgtg cgtggtttta cccaggatga cgcgcatatc ttctgtactg
     2461 aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat atgtacagca
     2521 cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa cgtattggca
     2581 gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca
     2641 tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc
     2701 tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac ttctccttgc
     2761 cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga gcgtaaggtt ccggtaatga
     2821 ttcaccgcgc aattctgggg tcgatggaac gtttcatcgg tatcctgacc gaagaattcg
     2881 ctggtttctt cccgacctgg cttgcgccgg ttcaggttgt tatcatgaat attaccgatt
     2941 cacagtctga ttacgttaac gaattgacgc aaaaactatc aaatgcgggc attcgtgtta
     3001 aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact ttgcgtcgcg
     3061 tcccatatat gctggtctgt ggtgataaag aggtggaatc aggcaaagtt gccgttcgca
     3121 cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag aagctgcaac
     3181 aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa ggcggaaaac
     3241 gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag gaagttcgct
     3301 taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct ctggagaaag
     3361 cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc
     3421 gtataatgga ttacggcaaa ttcctctatg aaaagagtaa gtcttctaag gaacagaaga
     3481 aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctggtaca gatgaaggcg
     3541 actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat aaagccaaaa
     3601 tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg gaagtgctta
     3661 atcgcgtgaa agacgatttg caagaactgg cagtggtcga atccttccca acgaagatcg
     3721 aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag
     3781 cactgtctgt ggagcctttg ggcttcacag gcattgttcg cctttgtttc gtttattaac
     3841 aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt gctgctaagc
     3901 gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg cgtcacattc
     3961 tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag
     4021 gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata agccgttaac gtttttaact
     4081 ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg tggtgttatt
     4141 gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg tgcgcgttct
     4201 cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc ttaccgcgac
     4261 cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca
     4321 cgtcagaatg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa
     4381 atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt caccgctctg
     4441 gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agagggagct agtctccctc
     4501 ttttcgtttc aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt
     4561 cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt
     4621 tttactttag cacctgactc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc
     4681 ctgaaagcct cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt
     4741 ctcatcaggc gtaattgtcg cagccagtct ggacacggcc agcgcggaga aaccggaccg
     4801 tacttaagta cgtgaggatt tcgagcactg cccgggacca aaatggcaag taaaatagcc
     4861 tgatgggatg ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac
     4921 atgtcacatc tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat
     4981 gttgccgcgt tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt
     5041 cagatgacga ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc
     5101 aatgaagcga aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc
     5161 gcagcactga atgcgcgtct ggcggcggaa acgattgatg tctccctgcc tggtcgtcgc
     5221 attgaaaacg gcgggctgca tccggttacc cgtaccatcg accgtatcga aagtttcttc
     5281 ggtgagcttg gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc
     5341 gatgctctga acattcctgg tcaccaccct gcgcgcgctg accacgacac tttctggttt
     5401 gacgctaccc gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc
     5461 cagcagccac cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag
     5521 actcacacgc cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt
     5581 actaacctga aaggcacgct gcacgacttc ctgcgtaact tctttgagga agatttgcag
     5641 atccgcttcc gtccttccta cttcccgttt accgaacctt ctgcagaagt ggacgtcatg
     5701 ggtaaaaacg gaaaatggct ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg
     5761 cgtaacgttg gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag
     5821 cgtctgacta tgttgcgcta cggcgtcacc gacctgcgtt cattctttga aaacgatctg
     5881 cgtttcctca aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt
     5941 tacgcgaatg ggtgaacccg gcgattgata gcgacgcgct ggcaaatcaa atcacgatgg
     6001 cgggcctgga agttgatggt gtagaaccgg ttgccggtag cttccacggc gtggttgtgg
     6061 gtgaagtggt tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga
     6121 atgtcggcgg cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc
     6181 tgcgtgtggc ggtagcgacc attggtgctg ttctgccggg tgatttcaaa attaaagccg
     6241 cgaaactgcg tggcgaaccg tctgaaggga tgctgtgctc cttctctgag ctgggaattt
     6301 ctgacgatca taacggcatt atcgagctgc ctgcggatgc gccgattggc accgacatcc
     6361 gcgaatacct gaaactcgat gacaacacca tcgaaatcag cgtgacacca aaccgtgccg
     6421 actgcttagg tatcattggt gttgcgcgcg acgttgccgt gttgaaccag ctgccgctgg
     6481 ttgaaccgga aatcgttccg gttggtgcga ccatcgacga cacgctgccg attgcagtag
     6541 aagcgccgga tgcctgcccg cgctatcttg gccgtgttgt aaaaggcatt aacgttaaag
     6601 cgccaactcc gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg
     6661 cagttgttga cgtcaccaac tatgtgctgc tcgaactggg ccagccgatg cacgctttcg
     6721 ataaagatcg cattgaaggc ggcattgtgg tgcggatggc aaaagagggt gaaacgctgg
     6781 tattgctcga cggtactgaa gcgaagctga atgctgacac cctggtcatc gccgaccaca
     6841 acaaggcgct ggcgatgggc ggcatatttg gtggcgaaca ctctggcgtg aatgacgaaa
     6901 cgcaaaacgt tctgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc
     6961 gtcgtcatgg cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac
     7021 tgcagcacaa agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgaag
     7081 cgggtccggt aattgatatc actaacgaag caacgctgcc gaagcgtgca accatcactt
     7141 tacgtcgtag caaactggat cgcctgatcg gccatcatat tgctgatgag caggtaacag
     7201 acattctgcg tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg
     7261 cgccgagctg gcgtttcgac atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg
     7321 tttacggcta caacaacatc ccggatgagc cggtacaggc aagcctgatt atggggattc
     7381 accgtgaagc tgacctgtcg ctcaagcgcg taaaaacgct gctcaacgac aaaggctatc
     7441 aggaagtgat cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg
     7501 ttgaagcctt actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttctc
     7561 tgtggaccgg cctgctggca accgtggtgt acaaccagaa ccgtcagcag aatcgtgtac
     7621 gcattttcga aagcggtctg cgtttcgtgc cagatactca ggcaccgttg ggtattcgtc
     7681 aggatctgat gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg
     7741 caaaagagac cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga
     7801 ccggtaaact gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc
     7861 aatccgcagc gatttatctg aaaggtgaac gtattggttt tgttggggtt gttcatcctg
     7921 aactggaacg taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca
     7981 agctcgcaga ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc
     8041 gtgacatcgc ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta
     8101 agaaagttgg cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg
     8161 gtgttgcgga ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta
     8221 cactcgaaga agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc
     8281 gattccaggc atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa
     8341 tatctgtttg ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt
     8401 ttcgaagaga tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt
     8461 aacttcgatc tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat
     8521 attcccatta cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg
     8581 gtcgaaaacg cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg
     8641 ccttttttct tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag
     8701 aacggatacc atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt
     8761 atgcctgtca gttttgatgc tgctggcgct tctcttaagc ctttgcgccg gtgaacaatg
     8821 gatctcgcca ggtgactggt ttactcctcg tggcgaactg ttcgtctggc aaattcgcct
     8881 gccacgtacg ctggctgtat tgctggttgg tgctgcgttg gctatatccg gcgctgtaat
     8941 gcaggcgttg tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc
     9001 aggcgtgggg cttatcgccg cggtattgct tgggcaaggg caactcccca actgggcgct
     9061 ggggctgtgt gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgcccg
     9121 tcgtcatctt tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag
     9181 tgcactaatg acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta
     9241 ctggatgatg ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt
     9301 gatccccgtg ttgttgtgga tctgttgtca gtccaggccg gtgaatatgt tagcacttgg
     9361 cgagatctcg gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgttggtcgc
     9421 agcgaccggc tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg
     9481 tctggtgatc ccacatattc tccggctgtg tggtttaacc gatcatcgcg tattacttcc
     9541 cggctgcgcg ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc
     9601 attagctgcc gcagagctgc ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt
     9661 tatctggtta ttgttaaaag caggacgtta gccgcaaaaa gacggtctat gattaaaagc
     9721 taagattttt actgaccaca cccaggagaa acgatgcaag attccattct gacgaccgta
     9781 gtgaaagata tcgacggtga agtgaccacg ctggagaagt acgccggtaa tgtgctgttg
     9841 attgtcaatg tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt ggagaatatt
     9901 cagaaagcct gggccgatcg tggttttgtg gtgctgggat tcccgtgcaa ccagtttctg
     9961 gaacaagaac cgggcagcga tgaagagatt aaaacttact gtaccaccac atggggggtg
    10021 acgttcccga tgttcagtaa gattgaagtt aatggcgaag gacgccatcc gctgtatcaa
    10081 aaattgattg ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt ctatgcccgt
    10141 atggtcagca aaggtcgtgc accgctgtac cctgatgata ttttatggaa ttttgagaaa
    10201 tttttagttg gcagggacgg attggtcatc cagcgttttt ccccggatat gacgccggaa
    10261 gatcccattg tgatggaaag cattaaactg gcgttggcaa aataatgtct attgtgatgc
    10321 agttacagga tgttgcggaa tctacccgcc tggggccgct ttctggcgag gttcgggctg
    10381 gggagatcct gcacctggtg gggccgaacg gcgcgggtaa gagtaccttg cttgcacgaa
    10441 tggccggaat gaccagcggt aagggaagca ttcagttcgc ggggcaacca ctggaagcat
    10501 ggtctgcaac aaaactcgcg ctgcatcgcg cctatctttc acaacagcag acgccgccgt
    10561 ttgcaatgcc ggtctggcac tacctgacac tgcatcagca cgataaaacg cgtaccgaac
    10621 tactgaatga tgtcgcaggg gcgctggctc ttgatgacaa actcggacgt agcaccaatc
    10681 aactttccgg cggtgaatgg caacgcgtac gtcttgctgc ggtggtgctt caaatcacac
    10741 cacaagctaa tcccgcaggc caattgctgc ttcttgatga gccgatgaac agtcttgatg
    10801 ttgcgcaaca aaatgcgtta gacaaaattc tgagcgcgct gtgtcagcaa ggactggcga
    10861 ttgtgatgag cagtcacgat ctcaaccaca cattgcgtca tgcgcatcgg gcgtggttgc
    10921 taaaaggtgg aaaaatgctg gccagtggac gcagagaaga ggtgctcacg ccggcaaatc
    10981 tggcacaggc ctatgggatg aattttcgcc gtctggatat cgaaggtcac agaatgctga
    11041 tttcgaccat ctgatttcat tagtatcttg aaaatcgggt aattacgacg ctaaattaat
    11101 gcaagaataa aaaacagagg aaccgtacgg aatgcgtttc tgccttattt tgatcacagc
    11161 actgtttctg gccgggtgta gccaccataa agcaccgccg ccaaatgcca ggctttctga
    11221 ttcgatcacc gttattgccg gtttgaacga ccagctacaa agctggcatg gcacgccgta
    11281 tcgttatggt ggcatgacgc ggcgcggtgt ggactgttcg ggatttgtgg ttgtgacgat
    11341 gcgcgatcgt ttcgatttgc agctgccccg tgaaaccaaa caacaagcct ctattggcac
    11401 gcaaattgat aaagacgagt tgctgccagg tgacctggtc tttttcaaaa cgggttccgg
    11461 acaaaatggt ttgcatgtag gtatttacga taccaacaac caatttatcc acgcctctac
    11521 cagcaaggga gtgatgcgtt cctcacttga taatgtctat tggcagaaaa atttctggca
    11581 ggcgagacga atctagtatg tcgaaaaatg gacggcgatg atacatcgcc gtccatttac
    11641 tcctgaatac agtagtattt tgcagacaga aattccgtaa atggattatt cgtttataat
    11701 caggataatc taaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt
    11761 actccttctg taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    11821 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    11881 gctatttcct gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc
    11941 atttctccag tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta
    12001 ccgaggagca acattggcaa ttattttctg aacagttgga attactaaaa tcatgccaac
    12061 acttttttat tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat
    12121 tattattaga cagggataat ttcgcaggcg aactattaaa atatccgttt attgaactgt
    12181 tgattaatga gaattacccg catttcaatg aaggaaaaga taatcgcgat cttttatcct
    12241 tatcgcaaat gtatcccctt gtgttaggta atttgggagc aggtaatagc acaatgaaag
    12301 cggtgtttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    12361 tcacgcatcg ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt
    12421 gcaattgcat catcgcgggg ggcattgata cgccagaaat attggcacag attataccct
    12481 ttgatttcca tgctctccag ggatgcctgt ggcctgcggt tcctataaat cagataacga
    12541 cgttggttca gcgataaccc tcctgtttgc tggtgtttag gacgagagta accgtctaca
    12601 ctatcaaaca ggaggatcta tgaccctgtc ttttattacc cgctggcgcg atgaattgcc
    12661 agaaacctat acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    12721 taccgaactg gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt
    12781 ctggggcggc gaaaacctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    12841 tcagtttggc gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca
    12901 actgcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    12961 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    13021 tgaggcgatg cattatctgg gcattccgac gacccgcgct ttaagtatcg tcaccagcga
    13081 ttcgccagtg tatcgggaaa cggtggagcc aggcgcgatg ctgatgcgtg tggcaccaag
    13141 tcatctgcgc tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg
    13201 tcagttagct gactttgcca ttcgtcatta ctggccacat cttgcagatg atgaggacaa
    13261 ataccgtctc tggtttaccg atgttgtcgc acgtaccgcc tcgttaattg cccaatggca
    13321 gactgtcggc tttgctcatg gggtgatgaa taccgacaac atgtcgctgc tggggctgac
    13381 gcttgattac gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca
    13441 ctcggatcat caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt
    13501 acagcgtctg gcgcagacat tgtcaccatt tgttgccgta gatggcctga atgaggccct
    13561 ggacagctat cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg
    13621 cttcatgacg gagctaaaag aggataacgc gctactgaat gaattattca gtctgatggc
    13681 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag
    13741 cgcggcgtca ccgctacgtg atgagtttat tgatcgtgca gcatttgatg actggtttgc
    13801 ccgttatcgg gggcgtttgc aacaagacga gattaccgat aatgagcgtc agcaactgat
    13861 gcaaagcgtt aacccggctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    13921 ggcagaaaag ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    13981 cagcgacaga gatgatgact atgtcagtcg tccacctgac tggggtaaac ggctggaagt
    14041 cagctgctcg agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga
    14101 gcagatattc ttgtccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    14161 ggctggtaat tcgccggatg ggctgcgggg aagcgg
//
LOCUS       DAPQZD010000121        14121 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPQZD010000121 DAPQZD010000000
VERSION     DAPQZD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.123"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(149..1798)
                     /gene="pgi"
                     /locus_tag="RO454_003523"
     CDS             complement(149..1798)
                     /gene="pgi"
                     /locus_tag="RO454_003523"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDY0935303.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2323..3672
                     /gene="lysC"
                     /locus_tag="RO454_003524"
     CDS             2323..3672
                     /gene="lysC"
                     /locus_tag="RO454_003524"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDY0935304.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3925..4197
                     /locus_tag="RO454_003525"
     CDS             3925..4197
                     /locus_tag="RO454_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDY0935305.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4198..5070)
                     /gene="rluF"
                     /locus_tag="RO454_003526"
     CDS             complement(4198..5070)
                     /gene="rluF"
                     /locus_tag="RO454_003526"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDY0935306.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5418..6365
                     /locus_tag="RO454_003527"
     CDS             5418..6365
                     /locus_tag="RO454_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDY0935307.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6435..7241
                     /locus_tag="RO454_003528"
     CDS             6435..7241
                     /locus_tag="RO454_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDY0935308.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7251..7658
                     /locus_tag="RO454_003529"
     CDS             7251..7658
                     /locus_tag="RO454_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDY0935309.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7658..8152
                     /locus_tag="RO454_003530"
     CDS             7658..8152
                     /locus_tag="RO454_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDY0935310.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8218..9015
                     /locus_tag="RO454_003531"
     CDS             8218..9015
                     /locus_tag="RO454_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDY0935311.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9026..9850
                     /locus_tag="RO454_003532"
     CDS             9026..9850
                     /locus_tag="RO454_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDY0935312.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNVVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9902..11131
                     /gene="sorE"
                     /locus_tag="RO454_003533"
     CDS             9902..11131
                     /gene="sorE"
                     /locus_tag="RO454_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDY0935313.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11513..12202
                     /gene="pepE"
                     /locus_tag="RO454_003534"
     CDS             11513..12202
                     /gene="pepE"
                     /locus_tag="RO454_003534"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDY0935314.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12292..13923)
                     /locus_tag="RO454_003535"
     CDS             complement(12292..13923)
                     /locus_tag="RO454_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDY0935315.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3399 a         3578 c         3709 g         3435 t
ORIGIN      
        1 ttaatcaatc gtactatgta ttaattgccg aatacgatgt acatatcggc atcgacctgt
       61 aggcctgata agacgcgacc gcgtcgcatc aggcatcggt tgccggatgc ggcgtgaacg
      121 ccttatccgg cctacatatc gacaatgatt aaccgcgcca cgctttatag cggttaatca
      181 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
      241 tacggttcgc cagttgttta cccagctcca cgccccattg gtcgaaggtg aagatgttca
      301 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgcc cccaggctga
      361 acggagtgat ttcacgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
      421 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
      481 ccacttcgcg ggatttacca aacgccagcg cttcggtctg agcaaagaag ttagacagca
      541 gtttctggtg atgatcagag agcgggttat gggtgatagc cggagcgatg aaatcgcacg
      601 gtaccatttt ggttccctgg tggatcagct gatagaacgc gtgctgaccg ttcgtgccag
      661 gttcacccca gataatcggg ccagtctggt aatccacaac cttaccgtta cggtcaacat
      721 acttaccgtt ggattccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
      781 ggtcatacgg cagaatcgct tcagtttccg cgccaaagaa attgttgtac cagatgccaa
      841 tcagcgccag cagtacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
      901 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
      961 acaggccaat cgctgaccac aaagaataac gaccgccgac ccagtcccag aattcgaaca
     1021 tgttggcagt atcaatacca aactcgccaa cggctttggc gttggtggaa agcgccgcaa
     1081 agtgtttcgc aacgtgcttc tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
     1141 gggcgttggt catggtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
     1201 tttccgggtt tacttttttc agcacttccg cgatgtgagt cccatcgacg ttagaaacaa
     1261 agtgcatgtt caggtggttt ttgtatggac gcagtgcttc agtcaccatg tacgggccga
     1321 ggtcagaacc gccgatcccg atgttcacta cgtcagtgat tgccttgccg gtatagcctt
     1381 tccactcacc ggaaataatc gcttccgaga aggttttcat cttctccagc accgcgttga
     1441 cttccggcat aacatctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
     1501 ctacgtgcag tacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
     1561 taatcgcgcc cgccagatcg cactctttcg ccagatcttg taatttcgcc agcgtctctt
     1621 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaaggtt gcggagaact
     1681 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
     1741 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
     1801 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
     1861 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagcgaa
     1921 gcatttatta gctgaactac tgaccgccag gtgtggatga aaaatccgca tgaccccacc
     1981 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
     2041 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
     2101 gggggtgcat cgccgaggtg attgaacggc tggctacgtt catcatcggc tacaggggct
     2161 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
     2221 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
     2281 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
     2341 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
     2401 gtgctttctg atgccaacgt acgtttagtt gtcctctcgg cttctgctgg tatcactaat
     2461 ctactggtcg ctttagctga aggactggaa cctggcgagc gattcgaaaa actcgacgca
     2521 atccgcaata tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
     2581 gagattgaac gcctgctgga gaacattacc gttctggcag aagcggcggc gctggcaacg
     2641 tctccggccc tgacagatga actggtcagc cacggcgagc tgatgtcgac cctgctgttt
     2701 gtcgagatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
     2761 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
     2821 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggc
     2881 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
     2941 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
     3001 tacaccaccg atccacgcgt ggtttctgca gcaaaacgca ttgatgaaat cgcgtttgcc
     3061 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
     3121 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
     3181 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
     3241 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
     3301 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacatcagaa
     3361 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
     3421 acgcaatccc tgctgatgga gctttccgca ctgtgccggg tggaagtgga agagggtctg
     3481 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
     3541 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
     3601 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcagaagct acattttaat
     3661 ttgtttgagt aaacacagta tggcccggaa gacattattt cgggccgtat tgtttttctt
     3721 gtcattatgc ccataaataa acgagcctgt actctgttaa ccagcgtctt tatcggagaa
     3781 taattgcctt taatttttta tccgcatctc taactaatta tcgaaagaga taaatagtta
     3841 agagaaggca aaatgaatat tatcagttct gctcgcaaac gaattcccgt acactgaact
     3901 ctctggctca ctgagggcac catcatggca ctcccccgta ttacccaaaa agagatgact
     3961 gaacgcgagc agcgtgaact taaaacactg ctggatcggg cgcggattgc gcatggtcgc
     4021 gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc
     4081 gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc ttataaaccg
     4141 gatcctgagg cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctgatta
     4201 acgccccttc tttttgcgcc ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc
     4261 gcctttttcc gccgtttttt ccatcttaac gactggacgt ttgatgccta ccgttttcgg
     4321 tttggccttc gctttaggtt ttgcctcgga agaggaattt tcaatgagct taaagagatc
     4381 gattaactca tcgtcggtta aatcgcgcca ctcacccagc ggaatgccgc ttaagctgac
     4441 gttcataatg cgcgtacgtt ccagcttttt cacttcgtaa ccaaagtgct cgcacatgcg
     4501 acgaatctga cggttcagcc cctgcaccag ggtaatgcgg aagacaaacg gtgcttcttt
     4561 tttgactttg cactttttag ttaccgtccc gaggatcggc actcccgccc ccataccacg
     4621 aataaattca tcggtaatcg gtttatcaac cgtcaccaga tactctttct catgatcgtt
     4681 acccgcacgg aggatcttat tcactagatc gccgtgattg gtgaggaaaa tcagcccctg
     4741 ggaatcttta tccaggcggc caatcgggaa cacgcgtttg ctgtggttaa cgaaatcgac
     4801 aatgttatcg cgctcgccat cttcggtggt gctgacaatg cctacgggct tgttcagggc
     4861 gataagtacc aaatcttcgg cttcccgagg ctcaatcaac tgaccgttta ctttcacaac
     4921 gtcgccggat ttcacctgat cgccaatggt ggctcgcttg ccattaagga acacattgcc
     4981 ttgctcgata tagcgatccg cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt
     5041 atttaaacgg actgatgagt cgggcagcat aaattctcct gtaaaacgcg gaatataccg
     5101 tacctcttag cggggaaaaa cagggttatg cggcaacgtc gaaattaaat gtgatctgcc
     5161 acgcctttta tcacaaaagt gtctccggaa gtacgatgca aatttgcctg gcaaaataag
     5221 gataatcaac aaattacgaa tcgctgcaca cctgtgctga tgactactac gcagagaaac
     5281 aaatgtgcaa gagaaagcgc tatttccccc catgcaaaga gtgaaaaatc gctttgaaag
     5341 gccatcatga tggggttata tcagatcaag cggtgagaaa gcgtatttgc acaaatgtgc
     5401 agaaccagga ggcggcaatg gaaaacagtg acgatatccg tttgattgtg aagattgccc
     5461 aactctatta cgaacaggat atgacgcagg cgcaaattgc gcgcgaactg ggtatttacc
     5521 gcaccaccat cagccgcttg cttaaacgag gccgcgatca gggaattgtc accatcgcca
     5581 tcaactatga ctacaacgaa aatctctggc tggagcagca agtgaagcaa aagtttggcc
     5641 tgaaagacgt tgtggtggtg tcaggaaatg atgaggatga agatactcaa ctggcgatga
     5701 tggggttaca cggcgcgcaa ctgctggatc gcttgctgga acctggtgat attgttggtt
     5761 tttcctgggg tcgcgcggtg agcgcactgg ttgaaaactt gccgcaggcg gggcaatcgc
     5821 ggcagttaat ttgcgtgccg attattggcg gcccgtccgg taaactcgaa agccgctatc
     5881 acgtaaacac attaacctac agcgcggcag cgaagctgaa aggggaatcg catctcgcgg
     5941 attttccggc tctgctggat aacccattaa ttcgtaatgg gatcatgcag tctcagcact
     6001 ttaaaaccat ctctgcctac tgggataatc tggatgtcgc cctggtggga attggctcac
     6061 cggccattcg cgacggcgct aactggcatg cgttttatgg tggtgaagag agtgacgacc
     6121 tgaatgcccg ccaggttgct ggcgatattt gctcgcgctt ttttgatatt cacggcgcaa
     6181 tggttgaaac gaatatgagc gaaaaaacac tctctatcga aatgaataaa ttaaagcagg
     6241 cacggtattc cattggcatt gccatgagcg aagaaaaata cagcggaatt gttggtgcac
     6301 tgcgtggaaa atatattaat tgtctggtaa cgaatagcag cacagctgaa ctattactga
     6361 aataaaaatc ttcccgatgt ttaccgtaat aaaaattacg gcgggtcctt attatctaaa
     6421 atcaggagtc tgttatgcaa acgtggttaa atttgcagga taaaatcatt attgtcaccg
     6481 gtggcgcatc cggcattggt ctggcgattg tggatgaatt attagcacaa ggcgcgaatg
     6541 tacagatggt cgatattcac ggtggcgatg gtcagtatga aagccataaa ggttatcagt
     6601 tctggccgac cgatatttcc agcgccaaag aggtaaatca tacggtagca gaaattatcc
     6661 agcgttttgg tcgcatcgac ggtctggtta acaacgccgg ggtcaatttc ccgcgtctgc
     6721 tggtcgatga gaaagcgcct gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa
     6781 tggtcaatat caaccagaaa ggcgtttttc tgatgtcgca ggcggtggcg cgacagatgg
     6841 tcaaacaaca tgatggcgtg attgtgaatg tttcctcaga aagtgggctg gaaggctcag
     6901 aaggccaaag ctgttacgcc gcgaccaaag ccgcgctcaa tagcttcacg cgctcctggt
     6961 cgaaagagct gggtaagcac ggtatccgtg tggtcggtat cgcgccgggg attctggaaa
     7021 aaacagggct gcgcacgccg gaatatgaag aggcgctggc gtggacgcgc aatatcaccg
     7081 tcgagcagct gcgtgaaggc tataccaaaa acgccattcc tattgggcgc gccggaagat
     7141 tagcagaagt ggctgatttt gtttgttatc tgctgtctga acgcgccagc tatatcaccg
     7201 gagtaaccac taacattgcg ggcggcaaaa cgcgcggcta aggaggcttt atggtcaatg
     7261 ccattttttg tgcgcatggc aaattagctt gcgccatgct ggaatcggtt cagatggttt
     7321 acggcgatgc cagggtcgag gcagttgaat ttgtgcccgg cgagaacgcc ggagacatcg
     7381 tagcaaagct ggaaaagtta gtaagcattc acaatcaaga tgagtggctg attgccgtgg
     7441 atttacagtg cggcagcccg tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc
     7501 tgcgggtaat cagcggcctt tctctgccgc tagcgctcga actggttgat aaccaggaca
     7561 gcatgaatgt tgatgaactg tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgtcg
     7621 tctggagaca ggtggcaaca gcggaggagg atttctgatg aacattacgc tcgcccgcat
     7681 tgatgaccgc ctgatccacg gccaggtcac taccgtctgg tctaaagtgg ctaacgccca
     7741 gcgcattatt atctgcaatg acgaagttta taacgatgaa gttcgccgga cattattgcg
     7801 tcaggctgcg ccaccgggaa tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt
     7861 gtatcacaac ccgcaatatc aggacgaaac cgttttttat cttttcactc gcccacagga
     7921 tgccttagca atggttcgcc agggggtaaa aataggtacc ctgaatattg gcggcatggc
     7981 ctggcgtcct ggtaaaaaac agttaaccaa agcagtttca ttagatgacg atgatattaa
     8041 cgcatttcat gagttaaata atcttggcgt catactggat ttacgcgtcg tagcgtcaga
     8101 tccctcaatc aatattatcg acaaaataaa tgaacagtta atcgcgaatt aaataatagc
     8161 gcctgtgtac atatgcttta acaggcaggg atgacgacac ttaaaaggtg acaaatcatg
     8221 gaaataagta ctctacaaat cattgctata tttctttttt cctgtattgc cggaatgggc
     8281 agcgtgctgg atgaatttca gactcatcgt ccgttaattg cctgtacggt gattggttta
     8341 attctcggtg atttaaaaac cggaattatg ctcggtggta cgctggaatt gatagctctc
     8401 ggctggatga acgtcggcgc ggcgcaatct ccggattctg cgctcgccag cataatctcc
     8461 gccattctgg ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct
     8521 gtggctgcgg caggccaggt gctgacggtg tttgcccgta ccatcaccgt agtgttccag
     8581 cacgcggcgg ataaagcagc ggaagaagca cggtttcgca ctctcgatat tctgcatgtc
     8641 tccgcgcttg gcgtgcaggc actgcgcgtt gctattccgg cactgattgt ctcgctgttc
     8701 gtcagcgctg atatggtgag caatatgctg agcgccattc ccgaatttgt gacccgtgga
     8761 ctgcagattg ctggcggttt tatcgtggtg gtcggttacg ccatggtgct tcgcatgatg
     8821 ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat
     8881 ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg ccctgctcta catccagtta
     8941 aatccacagt ggcgtaaagc tgaaccacat ccccagacca ccactatcac cgcccttgac
     9001 caacttgatg attaacggag ccatcatgga acagagaaaa attacacgca gcgatctggt
     9061 gagcatgttt ctgcgctcca acctgcaaca ggcatccttt aactttgaac gtattcacgg
     9121 gctgggtttt tgctacgaca tgatccccgc catcaaacga ctttacccat taaaagagga
     9181 tcaggttgcg gcgctcaggc gacacctggt gttcttcaat accacgccag ccgtatgtgg
     9241 cccggtcatc ggcgtcactg ccgccatgga agaggcgcga gccaacggcg cggaaattga
     9301 tgacggtacc attaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga
     9361 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
     9421 ttcgggcaac attctcggcc ccctgctgtt cttctttatt ttcaacgtgg tgcgcctggc
     9481 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
     9541 gggcggtaat ttgctgcaaa aactcaccga aggcgcgtcg attctcgggc tgtttgtgat
     9601 gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgcc
     9661 tgccgccgat ggtgccaccg tcaccataac cgtgcagaac atcctcgacc aactttgccc
     9721 tggtttgctg gcgctcggtc tgacgctgct gatggttcgt ctgctcaaca aaaaaattaa
     9781 cccggtatgg ctgattttcg ccctgtttgg tttagggatt atcggtaatg cgctgggctt
     9841 cctgtcctga ttcttcgccc cggcgcaacc gtcggggcca tcgttcaaca cgaggtggtt
     9901 tatgaaaaca acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga
     9961 ccttcctgaa atgcaggagg atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct
    10021 ctcttcctgg aaagaggcca atctgggtga aaatcataaa aaagtacccg acgatgtggc
    10081 gaccaatccc atcatcatcg gccacgaatt ttgcggcgat attctggccg tgggtaaaaa
    10141 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
    10201 cgaccgcccg gattgccccg gctactcctt cccgtgggta ggcggcgaag cgacgcatgt
    10261 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
    10321 ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
    10381 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
    10441 gatcctcggc ggcaccggac caatgggact gttggcgatt gattatgcgc tacatggacc
    10501 cgttaaccct tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcgcg
    10561 caagcactat ccatcagaac cgcaaacact gattcattat ctcaatgccg ccgatgcagc
    10621 atttgatacg ttaatggcac tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
    10681 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgtctgaa
    10741 tttcttcgcc ggaccgcagg ataagcactt cagcgcgcca attaatttct acgatgtgca
    10801 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcagc
    10861 ggtcaaatta attgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
    10921 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct
    10981 ggtgtatacc gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact
    11041 ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatacctgct
    11101 cgcccatgca gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg
    11161 ctggtcgccg cggcaacttc gcgacctgat gtgcggctga ttgctgaagc ggcacaatgt
    11221 gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg ctcaaattgg tggcaaagtg
    11281 cacattgttt atgccggatg cggaatgaag ccttattcgg cctacataat cttgaaaatt
    11341 caatatattg cagttatctg gtaggccagg taagcgtagc gcatcaggca atcctgtgtt
    11401 tgtcatcagt ctcagcgcct gtgcaatatg tgacatcgtg agccatgtca gtaagtggtt
    11461 aaattcgcag tggtagcatt ccttccgtgt tttcttacta accggagaag taatggaact
    11521 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacacg cgctgccgct
    11581 gattgctgaa cagttgcagg gtcgccgttc tgcggtattt attcctttcg ctggcgtaac
    11641 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
    11701 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
    11761 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
    11821 ggcacccatt actgacgtgg tgaaacgtgg cgcgctgtat attggctgga gcgcaggcgc
    11881 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattatcg atccgcaagg
    11941 tttcgatgcg ctaaatctgt tcccgctaca aatcaacccg cacttcacca acgcgctgcc
    12001 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
    12061 agaactgacg attattggtc tgccggaagg taactggatc acagtaagta aaggtcacgc
    12121 tacgctgggt ggcccgaaca ccacttatgt atttaaggct ggtgaagaag cggtgccgct
    12181 ggaagcgggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
    12241 gtgcctgatg caacgcttta cgcgtcttat caggccttca gggtcacggt attaatactc
    12301 gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg aacaaaacag
    12361 cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg tttcaatact
    12421 ctgcacgttt tgctggtgca ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat
    12481 gcgaaaacga tgtttgctgc ggcgcaaacg acgggcgctg gtaacatcgc cagagaagaa
    12541 aaccgacatt gccagtttta aattactgag caattgctca tagagcgcat ccagttcctt
    12601 caagccatca agcgaaaatg ctcgccttgc tgccagcgat ttgtcggcaa tttcgctgcc
    12661 catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga tgatctccgc
    12721 ccagcggcgc gactcttctt ctgccagttc ctcttttggc atccgcgcca gatacagctt
    12781 aatggcggta tagagaacgt tgatgtcatc agccagctta cgcagctctt tctcctgccg
    12841 tggctcgccg tgcatcactt tattcagccc ttccatcatc tgttccatgg cgtcgccaat
    12901 gcgcagggtt tcgcgggcag cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac
    12961 atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa taatcgtttt
    13021 gcaaaaccgt gccatcgggt caacgaatgg caacatgacc aggcagcgta caaggttgta
    13081 gaagacgtgg aaatagatca ccaattccgc ttttggcaat ggcaacttcc ccattgtctc
    13141 tgccagcaaa tgaacaaacg gcaggataat caggctaccc accagcttaa acagcaggct
    13201 acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca tcgccagcag
    13261 gccggaaccg aggtttgcac caatcaccag acaaagcgcc actgggaaag agataatgcc
    13321 tgcggcggtc agagtcgcgg tcagcagtac cgcggcaagg ctggagtagc tgataatggc
    13381 gaacatcgca ccaatcagcg catccagcaa aatgtcgcca gtcagcgagg caaagatcac
    13441 ctgaacgccg tttgcctgag tgatcggcgt tacggcctgc acaatcaact ccagcgccag
    13501 taaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg
    13561 tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg ataaatcgaa
    13621 ggtgagaata cgcgccatca gcgccgtccc gacatctgca cccagcacaa tcaccagcgc
    13681 aggcgcgaga gcaaccagat cctgagcgac gaacgaggtc accagcatgg tggtggcatt
    13741 actgctctgc accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg gctttttttc
    13801 gacgctccgg ctaaggacag tacgcagacg cgcgccgaag acgcgcatta cgcctgttcg
    13861 aacaatatgg gtcccccaga ccagcagggc gacggcagaa agcaggtgaa gcaaagttag
    13921 cacggaatca ggttctcctt tttgttatgc attttcctgt taagattttt ctaatcacgt
    13981 tgtttttatc caggcatttc tcttgtatgt actttcagta taagagattt atacaagaaa
    14041 gcatttaaaa aggcatttat ataaaaatcc acctgtatct gttgctattt ctgtcacact
    14101 ttcccctgtc gtttacacca g
//
LOCUS       DAPQZD010000122        14073 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPQZD010000122 DAPQZD010000000
VERSION     DAPQZD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.124"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            177..455
                     /locus_tag="RO454_003536"
                     /pseudo
     CDS             177..455
                     /locus_tag="RO454_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(506..751)
                     /locus_tag="RO454_003537"
                     /pseudo
     CDS             complement(506..751)
                     /locus_tag="RO454_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1124..1354)
                     /locus_tag="RO454_003538"
                     /pseudo
     CDS             complement(1124..1354)
                     /locus_tag="RO454_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1941..2096
                     /locus_tag="RO454_003539"
                     /pseudo
     CDS             1941..2096
                     /locus_tag="RO454_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(2093..2539)
                     /locus_tag="RO454_003540"
                     /pseudo
     CDS             complement(2093..2539)
                     /locus_tag="RO454_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(2726..2810)
                     /locus_tag="RO454_003541"
     tRNA            complement(2726..2810)
                     /locus_tag="RO454_003541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2774..2776),aa:Leu,seq:caa)
     gene            3006..4025
                     /gene="ahr"
                     /locus_tag="RO454_003542"
     CDS             3006..4025
                     /gene="ahr"
                     /locus_tag="RO454_003542"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDY0935316.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVI
                     LKADF"
     gene            4155..5657
                     /locus_tag="RO454_003543"
     CDS             4155..5657
                     /locus_tag="RO454_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDY0935317.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPMTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5818..6900)
                     /gene="lptG"
                     /locus_tag="RO454_003544"
     CDS             complement(5818..6900)
                     /gene="lptG"
                     /locus_tag="RO454_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDY0935318.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQIDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6900..8000)
                     /gene="lptF"
                     /locus_tag="RO454_003545"
     CDS             complement(6900..8000)
                     /gene="lptF"
                     /locus_tag="RO454_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDY0935319.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8267..9778
                     /gene="pepA"
                     /locus_tag="RO454_003546"
     CDS             8267..9778
                     /gene="pepA"
                     /locus_tag="RO454_003546"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDY0935320.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10134..10577
                     /gene="holC"
                     /locus_tag="RO454_003547"
     CDS             10134..10577
                     /gene="holC"
                     /locus_tag="RO454_003547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDY0935321.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10577..13432
                     /gene="valS"
                     /locus_tag="RO454_003548"
     CDS             10577..13432
                     /gene="valS"
                     /locus_tag="RO454_003548"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDY0935322.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13486..>14073)
                     /locus_tag="RO454_003549"
     CDS             complement(13486..>14073)
                     /locus_tag="RO454_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDY0935323.1"
                     /translation="SFLWSNTSFGIHRFKVKLDTTYCIKYAILAFLALLPFLAVAGYI
                     IFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHF
                     MSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYL
                     LGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3447 a         3646 c         3767 g         3213 t
ORIGIN      
        1 ccctatcagg ctgtctctta tacacaaatc cccacccggg gatttgtgat gtctgtcagt
       61 ctgctttagg ggggactctt tccattcggt agtttccttc ctccccggca gcgatttttt
      121 gaactttttc ttcacccgtt gatcttctct tatatttcat tttcttcggg tctgttatgg
      181 tactttctga ttgttattca tgggataacg agcagtttgg acatgcccgt ctcggtgatc
      241 cgcgtcgtac acgacgcctt gtgtcactgg cctcttctct cgctcagcat gcaggtcttt
      301 ctattgtgaa atcatcccac tccactgctc aggttgaaag tgcgtaccgc ctgatacgta
      361 acccgtctgt gtccccggaa gccattgcct aagccggatt taccgccact gcaagggcat
      421 gtgaggctca tccgctgctc ctcgcactgg aagacaccac cactaatcgt aagcgtacag
      481 cgagggccgt attgacgggg atgtgttatt cagctggcag tgctatacgc cacggaagca
      541 gttcgctgac ccggttgacc ggccagtctg ctatgacgcc aagcacatgg cgaaggtagc
      601 tttctggatc cacgtcattc agtttgcacg tcccgatcag gctgtacagt agcgctcccc
      661 gctcaccacc atggtcagag ccgaagaaca ggaagttttt acgacccaga ctgaccgtca
      721 acgccacaat ctttgactgt ttgacattgt catgcccgca ggccagcgaa tctaaagaca
      781 tcagactgct tgcatcagcc agttgcgtat ctggctatgt cccttaattg gtaagggttc
      841 gaggaactca acattaacgc atttttccgc ccatcaccgg cagtgtatat gaacgattgc
      901 actcttacga tggttgattt atttggttgc gtaggggcta gctagtctgg taccggaggc
      961 tggatctgaa gtactgaccc caagaataac gccgggcaga ctcatattct gacccattgt
     1021 cagcccgcgt gtttactgct ctagctcatt tcctgttgtc aggcgttcaa ttcccaactg
     1081 caggttatgg cccggtgcac aaacacctga cggcaggtat cacttaccca aagaagataa
     1141 agccctcaag aggcagtaac tctgccaacc tctcctccgg ccactccggg cagacgcgtt
     1201 aggacgtccg tcagccaagc atgtggctcc agactgctgt ttccagaagg ctcattatct
     1261 gtgccgctcg gttcccggcc atcagtgaac cagcgaacaa ccaggccttt cgtcccataa
     1321 cgaccggacg gatagcacgc tcgcagatat tgttgtgtgt gccttgaaat gcacgatctt
     1381 tccgtcggtg aaagtccgcc ccgactaact ctcgctccaa ctggaaggac aggaagcggt
     1441 ccaggaggta acaaaagggc tgaatctctc cctgaaacga cttcttcagg aagtcaatgg
     1501 gttatgcggg ccgtaacgtg agtgaacact gagcagccat cgaaatgccg tcgatggtgc
     1561 cgatacgcca caataacggg gaaggccgac gatgaccggg aagagagtga gaaaggcatc
     1621 ggtcataccc accggggtag tggtgtcagc atgcataaca attgatcgat cgtgcaacaa
     1681 gagaagtcca taacgatgcc tgtccgggtg acgggcaaca acgcctcccg agggggtgaa
     1741 tcggttgtta tggatggcag atggggtgta gtagtgaaaa tactgagtaa tgtcatagga
     1801 gcgaaggccc cctgttgcag taccagtgta acatgcagga agagcccggg gattgccatc
     1861 agcctcacaa ccccgctcga accggttcag aacctacaga aaatactaca ggagaaagca
     1921 aagtgaacct ccccgtgttt cctgtttatc attttctggt cagtgccgcg attctggtat
     1981 tcgtggtgat tttctggcgg acacaccatc gcgaccaccg taactggctg gctctacggc
     2041 tattcgtatt atgctctgta aaccgttggc cattgcggat ggttaaagga acaataggtg
     2101 ctaataaatc tcgagttttt aactcagcga tattacgttc accaagcgct gcaaaaagat
     2161 tatcttcaag actttttagc acacgatggg cgtgatcttc agaccacttt ttattggtgc
     2221 catgccactc aatcgcgact tctttaaagg ttcgtgcttt actctgttca accttatcat
     2281 ttttcttttt gtctcccgga tcgacgccat tcgcaagcag cttacgcgcc tcgtcacgac
     2341 gtactctggc atccgctagt gtgatttcag gataaacccc aagtgccagc attttttgct
     2401 ttccctcata acggtactgc aaacgccagt acttagaacc atttggatgg acaagcagat
     2461 gcataccgcc accatcagtc agcttatatt gcttatccgt tggcttggct gctctgactt
     2521 tgatatctgt taatgccatg cgtattccct ccctgttggt acaagcatta tcgaaccgga
     2581 aataccatca tctgtaccaa catatgttgg tagatgtacg ttgatatcgg ttgaccttga
     2641 gagagttaat atagcgggaa agatagataa acactggatt tcaggcataa aaaaagacct
     2701 cagttgaggt ctatttacat acttttggtg ccgaaggccg gactcgaacc ggcacgtatt
     2761 tctacggttg attttgaatc aactgcgtct accgatttcg ccacttcggc actgaagagg
     2821 tatgcggaaa acgttgtgga ttatacctgt cgcacgccac catgcaagcg ccagcttgct
     2881 cgcacctcgc taagtgctga aattttcagc attgcataca gcgatgtgta acctttgtca
     2941 cactccaggc accccgccct gccatgctct acacttccca gacaacacca gagaaggacc
     3001 aaaaaatgtc gatgataaaa agctatgccg caaaagaagc aggcggcgaa ctggaagttt
     3061 atgagtacga tcccggtgaa ctgaagccac aagatgttga agtgcaggtg gattactgcg
     3121 ggatctgcca ttccgatctg tcgatgatcg ataacgaatg gggattttca caatatccgc
     3181 tggttgccgg gcatgaggtg attggtcgcg tggtggcgct cgggagcgcc gcgcaggata
     3241 aaggtttgca ggtcggtcag cgtgtcggga ttggctggac ggcgcgtagc tgcgggcatt
     3301 gcgatgcctg tattagcggt aatcagatca actgtgagca aggtgcggtg ccgacgatta
     3361 tgaatcgcgg tggctttgcc gagaagttgc gtgcggactg gcaatgggtg attccactgc
     3421 cagaaaatat tgatatcgag tccgccgggc cgctgttgtg cggtggtatc acggtcttca
     3481 aaccactgtt aatgcaccat atcactgcca ccagccgcgt tggggtaatt ggtattggcg
     3541 ggctggggca tatcgctata aaacttctgc acgcaatggg atgcgaggtg acagccttta
     3601 gttcgaatcc ggcgaaagag caggaagtac tggcgatggg tgccgataaa gtggtgaata
     3661 gccgcgatcc gcaggcactg aaagcactgg cggggcagtt tgatctcatt atcaacaccg
     3721 tcaacgtcag cctcgactgg cagccttatt ttgaagcgct gacgtacggc ggtaatttcc
     3781 atacggtcgg tgcggttctc acgccgctgt ctgttccggc ctttacgtta attgcgggcg
     3841 accgcagcgt ctctggttct gctaccggca cgccttatga gttgcgtaag ctgatgcgct
     3901 ttgccgcccg cagcaaggtt gcgccgacta ccgaattgtt cccgatgtcg aaaattaacg
     3961 acgccattca gcatgtacgc gatggtaaag cgcgctaccg agtgatcctg aaagccgact
     4021 tctgatccct ttgccgggtg cctgtcgccc ggcacttaaa tctctttgga aggcgtctcg
     4081 caatctgctg aatttagcgg tgaagagtgc gcagccgttg cctatactcc aggcaggaaa
     4141 ctggaggaaa tctcatgagc gaacccctgt taattgcccg cacgccggac acagaactat
     4201 ttttactgcc gggaatggct aaccgtcacg ggctgattac tggcgcaacg gggacgggta
     4261 aaactgtcac gctgcaaaaa ctggcggagt ctctatcgga aatcggtgtt ccggtgttta
     4321 tggccgatgt gaaaggcgat ctgaccggca tcgcgcaggc aggaacggcg tcggaaaaac
     4381 tgctcgcaag gcttaaaaat atcggcgtca atgactggca accgcatgcc aatccggtgg
     4441 tggtgtggga tatctttggc gagaaaggcc atccggtgcg ggcgacggtt tcggatctgg
     4501 ggccgctgtt gttggcgcgg ctgttgaatc tcaacgatgt gcagtctggc gtgctggata
     4561 tcatcttccg cattgctgac gatcaggggc tgttactgct cgactttaaa gatttgcggg
     4621 cgattaccca gtacatcggc gataacgcca aatctttcca gaatcagtac ggaaatatca
     4681 gtagcgcatc ggttggtgcc atccagcgcg ggctgttgtc gctggaacag caaggcgcag
     4741 cacacttctt tggtgagccg atgctggata tcaaagactg gatgcgcacc gatgccaacg
     4801 gtaaaggcgt tatcaatatc ctcagcgccg agaagcttta tcagatgccg aaactgtatg
     4861 ccgccagcct gttgtggatg ctctcggagt tgtatgaaca attgccggaa gcgggcgatc
     4921 tggagaaacc aaaactggtg tttttcttcg acgaggcaca tctgctgttt aacgatgcac
     4981 cgcaggtact gctggataag attgagcagg tgataaggct tattcgctca aaaggcgtgg
     5041 gcgtctggtt cgtttcgcaa aatccgtccg atattccgga taacgtgctc ggccagctcg
     5101 gtaatcgcgt tcaacacgct ttgcggactt ttacgcccaa agatcagaaa gcggtaaaag
     5161 ctgcggcgca aaccatgcgg gtcaatccgg catttgatac cgaaaaggcg attcaggaac
     5221 tgggcaccgg cgaggcgttg atctcttttc tggatgcgaa aggaagccct tctgtggtgg
     5281 aacgggcgat ggtgattgcg ccttgttcgc ggatgggacc gatgacggaa gatgagcgta
     5341 atggcttgat taatcactct ccggtgtatg gcaagtacga ggatgacgtg gaccgggaat
     5401 ccgcctatga gatgttgcaa aaaggcgtac aggcaagcgc cgagcagcaa aataatcctg
     5461 ccgtgaaagg gaaagaggtg gcggtggatg acggcattct tggtggattg aaggatattt
     5521 tgtttggcac taccggacca cgcggcggga agaaagatgg tgtggtgcaa acaatggcga
     5581 aaagcgccgc ccgccaggtg acgaatcaga ttgtacgtgg gatgttgggg agtttgctgg
     5641 ggggaagaag aaggtaagtc aggcgcgatt tgtctttgcc tgatgcgacg ctggcgcgtc
     5701 ttatcatgcc tacaccactg caatatattg gattttcatg atttttcagg catgaacgaa
     5761 gcacactttg ctaacaataa taaagggagc tttcgctccc tttattcgtt cattcggtta
     5821 cgattttctc attaacagcc acaggctgat taagaagaag ctggcgcttg gcaacagtgc
     5881 gccgatgatc ggcgggatgc cataaaccaa cgtcagcggg ccgaagatct ggtccagtac
     5941 atagaagaca aaaccgaagc tgataccggt gaccacacgc acacccatcg gtacgctacg
     6001 cagtgggcca aagatgaacg acagcgccat cagcatcatc accgccacgg atagcggctg
     6061 gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgatttcag
     6121 atacttcaca tagttgtgca aaccgctgat agagagtgca tccggatcca gcgccaccac
     6181 gcccagtttg tcaggcgtga ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt
     6241 gatctgtttc ggattggtca gatcagattc atcaatctgc gacagacgcc agactttatg
     6301 ttccgggtca aacttcgcgg tagcggcata gcgtacggat tgcagacgac gattctcgtt
     6361 aaaggcataa atactgatgc cacctaactc ttcgtcacct ttaacccgct caatgtagac
     6421 gaagttgttg ccatctttcg cccataaacc ttgctgggta gagagcaacg agccgccgta
     6481 catcgcctgc gcacggtagt tacgcgccat ctgctcgccc tgcggcgcga cccattcgcc
     6541 aatcgccatc gtcagcaaga ccagcggaat ggcggttttc atcaccgaca gcgccacctg
     6601 catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc
     6661 aagaccaagc aacgccccaa gcaaagccgc catcgggaag aatatttgca catctttcgg
     6721 cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg tagctcccct gcccggcttt
     6781 tttcaactga tcgacaaatt tgataatgcc cgacagcgac accagcatga acagcgtcat
     6841 catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat
     6901 cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac agtgtcccaa
     6961 aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat ccacagcgtc
     7021 gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaaaagt
     7081 agatacagca gcatggctgg cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg
     7141 ctcaacggta caaccataag tgccatcata aacacggtga ataccaacgt gatacgccag
     7201 ttcagttctg cgcgggcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg
     7261 tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg
     7321 aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg
     7381 agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc
     7441 acagaaggtc gtgcattacc ttttggtcga atttgtgcga ggaacacatc tttgaaatcg
     7501 ctgccgtcaa cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc
     7561 ccttgcgcca gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga
     7621 tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat
     7681 accgcaagga tcattgccgc tttcaccaga accgctttgc tcaggccgca ggcatgcatt
     7741 accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg
     7801 cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc
     7861 agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag
     7921 aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc
     7981 agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt
     8041 agcttgtttc atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga
     8101 taattatact caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa
     8161 cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc
     8221 tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt
     8281 aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc
     8341 acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc
     8401 cctgctacgt cggggcgaac tggaaggaaa accggggcag acattgttgc tgcaccatgt
     8461 accaaacgtg ctttctgaac gcatcctcct tattggttgc ggcaaagaac gtgagctgga
     8521 tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc
     8581 aatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa
     8641 agtgcgtcag gctgtcgaga cggcaaaaga gacgctctac agtttcgatc agctgaaaac
     8701 gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg
     8761 tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggattaa
     8821 agcagcaaag gatcttggca atatgccgcc gaatatctgt aacgccgctt acctcgcttc
     8881 acaagcgcgc cagctggctg acagctacag caagaatgtt atcacccgcg ttatcggcga
     8941 acagcagatg aaagagctgg ggatgcattc ctatctggcg gtcggtcagg gttcgcaaaa
     9001 cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat
     9061 cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctcgatca agccttcaga
     9121 aggcatggat gagatgaagt acgatatgtg cggtgcggca gcggtttacg gtgtgatgcg
     9181 tatggtcgcg gagctacaac tgccgattaa cgttatcggc gtgttggcag gctgcgaaaa
     9241 catgcctggc ggacgagcct atcgtccggg cgatgtgtta accaccatgt ccggccaaac
     9301 cgttgaagtg ctgaatactg acgctgaagg ccgcctggta ctgtgcgacg tgttaactta
     9361 cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg acgctgaccg gtgcctgcgt
     9421 gatcgcgctg ggtcatcata ttactggtct gatggcgaac cataatccgc tggcccatga
     9481 actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga
     9541 gtatcaggaa caactggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg
     9601 tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtaca actgggcgca
     9661 cctggatatc gccggtaccg cctggcgttc tggtaaagca aaaggcgcaa ccggtcgccc
     9721 ggtggcgttg ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaatt
     9781 gcgtcaggca aggctgttat tgccggatgc ggcgtgaacg ccttatccga cctacacagc
     9841 actgaactcg taggcctgat aagacgcgca agcgtcgcat caggcaatgc tgttattgtc
     9901 ggatgcggca tgaacgcctt atccgaccta cacacagcac tgaactcgta ggcctgataa
     9961 gacacaacag cgtcgcatca ggcaatgctg ttattgtcgg atgcggcgtg agcgccttat
    10021 ccgacctaca cacagcactg aactcgtagg cctgataaga cacaacagcg tcgcatcagg
    10081 cgctgcggtg tatacctgat gcgtatttaa atccaccaca agaagcccca tttatgaaaa
    10141 acgcgacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
    10201 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgtg ctcatcgcct
    10261 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
    10321 gctttgttcc gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg
    10381 cctggccgca aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct
    10441 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
    10501 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
    10561 cggcaacctg gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta
    10621 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
    10681 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
    10741 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
    10801 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
    10861 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
    10921 ggaatggaaa gcggaatccg gcggcacgat tacccgtcag atgcgccgtc tcggcaactc
    10981 cgtcgactgg gagcgtgaac gcttcacgat ggacgaaggc ctgtccaatg cggtgaaaga
    11041 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg
    11101 ggatccgaaa ctgcgcaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg
    11161 ttcgatgtgg cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga
    11221 ttatctggtg gttgcgacta cccgtccgga aaccctgttg ggcgataccg gcgtggccgt
    11281 taacccggaa gatccgcgtt acaaagacct gattggcaaa tatgtcattc taccgctggt
    11341 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
    11401 cgtgaaaatc actccggcgc acgactttaa cgactatgaa gtgggtaaac gtcacgcgct
    11461 gccgatgatc aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga
    11521 taccaaaggt aacgaatctg acgtttattc cagcgaaatc ccggcagagt tccagaaact
    11581 cgagcgtttt gctgcacgta aagcagtcgt tgctgcggtt gacgcactcg gcctgctgga
    11641 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tagttatcga
    11701 accaatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcggttga
    11761 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc
    11821 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    11881 ccctgcatgg tacgacgaag caggtaacgt ttatgttggc cgcaacgaag aagaagtgcg
    11941 caaggaaaat aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac
    12001 ctggttctct tctgcactgt ggaccttctc tacccttggc tggccggaaa acaccgacgc
    12061 cctgcgtcag ttccacccaa ccagcgtgat ggtatccggc ttcgacatca tcttcttctg
    12121 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    12181 ggtgccgttc cacactgttt acatgaccgg tctgattcgt gacgacgaag gccagaagat
    12241 gtccaaatcc aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc
    12301 agaactgctg gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    12361 taagcgcacc gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg
    12421 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgcct
    12481 ggaaggttac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    12541 cacggaaggt caggattgcg gcttcaacgg tggtgaaatg acgctgtcgc tggcggaccg
    12601 ctggattctg gcagagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt
    12661 ccgcttcgat atcgctgcag gcattctgta tgagttcacc tggaaccagt tctgcgactg
    12721 gtatctggag ctgaccaagc cggtaatgaa cggtggaacc gaagccgaac tgcgcggtac
    12781 tcgccatacg ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    12841 gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    12901 catcatgctg cagccgttcc cgcagtacga tgcatctcag gttgatgaag cggcgctggc
    12961 cgacaccgag tggctgaagc aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa
    13021 catcgcgccg ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg
    13081 tcgcgtaaat gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt
    13141 gctgcctgcc gatgacaaag gtccggtttc cgttacgaag attatcgacg gcgcagagct
    13201 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga
    13261 agtggcgaag atcgaaggcg aaatcagccg catcgagaac aagctggcga atgaaggctt
    13321 tgtcgcccgc gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc
    13381 ggaagcgaaa gcgaagctga ttgaacagca ggctgttatc gccgcgctgt aatcaacacc
    13441 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    13501 agaaggcata attccccgtg aaatactggc taagaagcct ttatctggtt gttcttcttt
    13561 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    13621 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    13681 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    13741 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    13801 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    13861 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacacagaac tatcatacgc
    13921 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagctaa
    13981 aaatgcgaga atggcatatt ttatacaata cgtagtatct aatttcacct tgaaacgatg
    14041 tataccgaaa ctggtattgc tccagagaaa act
//
LOCUS       DAPQZD010000123        14068 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPQZD010000123 DAPQZD010000000
VERSION     DAPQZD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.125"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            8..3937
                     /locus_tag="RO454_003550"
     CDS             8..3937
                     /locus_tag="RO454_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0935324.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLAIFIVSALS
                     YGAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPD
                     FSPVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLD
                     KFVVETAGATEHADFNLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYN
                     PLLRSASMFQLNWNNMYASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKV
                     FSSKDTWAFFARYDQNTVDILKNTFTQEVNLNGQKMTVNNKNIAINDKITAIELTKSN
                     KNKDLKFHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDW
                     SVKGEANDNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLG
                     QADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQN
                     PSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHA
                     IFREGPRHCYVPGVLCDKDYVTDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHF
                     RFKTLNLENSEFTTARNSVVEGDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQ
                     DVREGRSADDGSSSYTGKITLQKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGV
                     FVNSTLSVRDGGHLTAQKGLYSNGRVQIGKKGTLSLSGTPENGADNTWMPVLTYMTEG
                     YDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNA
                     AYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATG
                     SDFVLRTDLKDADKISITEKASGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIF
                     KAGTRVTGFSRVTPTLRVDTTGGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTE
                     VNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLF
                     TGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFA
                     GLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMK
                     NKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGE
                     KDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANFRYMF"
     gene            4151..4423
                     /locus_tag="RO454_003551"
     CDS             4151..4423
                     /locus_tag="RO454_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0935325.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            4651..4947
                     /gene="ghoS"
                     /locus_tag="RO454_003552"
     CDS             4651..4947
                     /gene="ghoS"
                     /locus_tag="RO454_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDY0935326.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            4975..5148
                     /gene="ghoT"
                     /locus_tag="RO454_003553"
     CDS             4975..5148
                     /gene="ghoT"
                     /locus_tag="RO454_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDY0935327.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(5267..6784)
                     /gene="lysS"
                     /locus_tag="RO454_003554"
     CDS             complement(5267..6784)
                     /gene="lysS"
                     /locus_tag="RO454_003554"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDY0935328.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7021..8478)
                     /gene="dtpC"
                     /locus_tag="RO454_003555"
     CDS             complement(7021..8478)
                     /gene="dtpC"
                     /locus_tag="RO454_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDY0935329.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(8537..10684)
                     /gene="cadA"
                     /locus_tag="RO454_003556"
     CDS             complement(8537..10684)
                     /gene="cadA"
                     /locus_tag="RO454_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDY0935330.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(10764..12098)
                     /gene="cadB"
                     /locus_tag="RO454_003557"
     CDS             complement(10764..12098)
                     /gene="cadB"
                     /locus_tag="RO454_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDY0935331.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(12464..14002)
                     /gene="cadC"
                     /locus_tag="RO454_003558"
     CDS             complement(12464..14002)
                     /gene="cadC"
                     /locus_tag="RO454_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDY0935332.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         4006 a         3180 c         3324 g         3558 t
ORIGIN      
        1 cttacccgtg aacagaatat attcacttaa gtattgtcac cttactcaag ggcttatagc
       61 tgtatcagaa ttagcatccc gagtatcctc taaaaccggt cgaaaacttc tcgctatatt
      121 tatagtttct gccttgtctt atggtgcggc tgaatatgcc tgtgcagcac agatggacac
      181 ccgaaatttc tggatccgtg attatcttga tctggcacag aataaaggtg catttcagcc
      241 cggagcatac ggggtaaaaa cgccattaaa aaatggggga gaattcagtt ttcctgaagt
      301 aacaatccct gatttttctc ctgtatccgc taaaggtgca acaactgcta ttggtaatgc
      361 ctacagtgtt acagcaagtc ataatggcac tattcaccat gccattaaaa ctcagacatg
      421 gggacagtca gattatcatt atgttgatcg tgtgaccaaa ggtgactttg cggtccagcg
      481 tctggataag tttgttgttg aaacagcggg tgcaacagag catgctgatt tcaacttatc
      541 agcagcagaa gcactagagc gttatggtat tgaattcaat ggaaagaaac aaataatcgg
      601 tttccgcgtt ggagctggtg caaccggtgt tacatcttat ggggtgggac agacatacaa
      661 tccattatta cgtagtgcct ccatgtttca gttaaactgg aacaatatgt atgcaagtaa
      721 taataccggt gggttttata atgaagtgac aggaggtgac agcggttccg gattttactt
      781 gtatgataat cagaaaaaaa aatgggtcat tttggggaca ctgacaggta aagtattctc
      841 cagtaaggat acctgggcct tttttgctcg atatgatcag aacactgtcg atatcctgaa
      901 aaatactttt acccaagagg tgaacctcaa tggtcagaaa atgacagtta ataataaaaa
      961 tattgccatt aacgataaaa taactgctat tgaactgacc aagagtaata aaaataaaga
     1021 tttgaaattc catggtggcg ggagcattga gctcaccgat aacctgaact caggaaccgg
     1081 aggattgatt tttgatgagg gacaacatta ttcggttatt gggaaagata aagcctataa
     1141 aggggcgggt gttgagatcg gaaaagatac ggttgtcgac tggtcggtaa aaggggaggc
     1201 aaacgataac ctgcacaaaa caggggccgg gacactgaat gtcaatgtgg cccaggggaa
     1261 taacctgaaa acaggtgacg gtaccgtttt tcttaatgca gaaaaggctt tcaatgctat
     1321 ctatgttgcc agtggccgtg gaacggtcaa actggggcag gccgatgcgc tggataaaaa
     1381 tagtgattac agaggtattt attttactag tcgtgggggg actctggatt taaacgggtt
     1441 cagccagtcg ttcaagaaga tcgcggcaac tgatgttggt accattatca ccaatacttc
     1501 tgataaaaca gcgacccttt ccctacaaaa cccctcccgc tatgtctatc acggtagtat
     1561 cacgggaaat acgaatatcg aacacactgg gacacagaaa agtgctgaca gcagtctgat
     1621 tattgatgga aacattaata cacgcaatga cattactgtg cggaattccc agctcagact
     1681 tcaggggcat gccacatcac atgcaatatt ccgcgagggg cctcggcact gctatgtccc
     1741 cggagttctt tgtgacaaag attatgttac tgattttgcc agactggaaa gtgaggcaaa
     1801 taagaaaaat aacagtgcct ataaaacaaa taatcaggtg gcttcttttg accaacctga
     1861 ctgggaaacc cggcatttcc gatttaagac tctgaatctg gaaaactcag aattcacaac
     1921 tgcacgtaac tcagttgttg agggtgatat tgtcgcatcg aattcaacgc tgaaactggg
     1981 gggcgacgtt ccggtgttca ttgatatgta tgatggcatc aatatcaccg gtaatggttt
     2041 tggcttccgc caggacgttc gtgaaggacg ctcagcagat gatggcagta gcagctatac
     2101 aggcaaaatt acactgcaaa aaggctccac gctggacatc aacaaccggt tcattggcgg
     2161 tattgaagcc catgacagta aggtaaacgt cacctcaccg gatgccctgc tgcagaacag
     2221 tggtgttttc gtgaattcca ccctttctgt ccgtgacggc ggtcatctga cggcacaaaa
     2281 agggctctac agtaacggcc gggttcagat tggaaagaaa ggtacgcttt ccctgagcgg
     2341 cacgccggaa aatggcgctg ataatacctg gatgcccgta ctgacataca tgacagaagg
     2401 ctatgattta accggtgata acgctacgct gaacatcagc cagcaggcgc atgtttccgg
     2461 ggatgttcat gcaaccagtt catccagcat tcgtattggc tccgaaaacc ctggctcagt
     2521 ttcctcttct gtctcccctg ttctggctgc cgggttgttc agcggatata acgcggcgta
     2581 ctacggtgcc atcaccggcg gtaaggggaa cgtcagtatg aataatggcc tgtggcagct
     2641 gaccggagat tccgacatca acagtctgac gacccgtaac agccgggtac agtctgaaga
     2701 aaacggtgcc ttccgtaccc tgacggttaa gacacttgat gccacgggca gtgattttgt
     2761 cctgcgcact gatctgaagg acgctgataa aatcagtatt acggagaaag ccagcggttc
     2821 agacaacacc ctgaatgtca gctttatgaa gaacccgtct ccgggacagt ccctgaatat
     2881 cccgctggtc agtgcaccgg ccggaacatc aggggatatc tttaaggccg gcacccgggt
     2941 gacaggcttc agtcgtgtga cgccgacgct gcgtgttgac accactggcg gcagtacgaa
     3001 gtggattctg gatggtttca ggacggaagc tgataaagcg gctgcagcga aggcgaacag
     3061 tttcatgaat gccggctaca aaagctttat gacggaagta aacaatctga acaaacgtat
     3121 gggggagctg cgtgacacga acggtgatgc cggtgcctgg gcccgtatta tgaacggcgc
     3181 cggttcagcc gatggcggat acagtgataa ctacacccac gttcaggtcg gttttgacaa
     3241 aaaacatgcg ctggacggtg ttgacctgtt caccggtgtc acgatgacct ataccgacag
     3301 cagtgcagac agtgatgcgt tcagcgggaa gacaaaatcc gtggggggag gtctgtatgc
     3361 ttcagcactg tttaactccg gtgcctacat tgatttgatt ggtaaatata ttcaccatga
     3421 caatgactac acaggtaact ttgccggtct ggggacgaag cactacggaa cccactcctg
     3481 gtatgccgga gcggaaacgg gttaccgtta tcacctgacg gaagacacat ttattgagcc
     3541 tcaggccgaa ctggtttacg gcgcagtgtc cgggaaaaca ttccgctgga aagacggtga
     3601 gatggacctg agtatgaaga acaaggattt cagcccgttg attggcagaa cagggattga
     3661 gctgggcaaa accttcaggg gtaaggactg gagtgtgaca gcccgtgccg gaaccagctg
     3721 gcagtttgac ctgctgaata atggcgagac agttcttcgt gatgcatccg gagagaaacg
     3781 gatcaaaggg gagaaagaca gcaggatgtt gttcaatgtt ggcatgaacg cacagataaa
     3841 ggacaacatg cgctttggtc tggagtttga gaaatccgcc tttggtaaat acaacgtgga
     3901 taacgcaata aacgcgaatt tccggtatat gttctgacaa ggagaggccc ctgaagaggg
     3961 gcctgtagtg atgttactcc cgggttaatg cagctagtaa aggtatggct ctgactaccc
     4021 tggaaagtgg tgcccatcgc atcactacaa attctggcaa ttgcgtacgt ggtaacggca
     4081 agttatttaa cctcaaacgt acgtgcccga gcggtgcgct gaaataccgt cataaataag
     4141 cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaggg
     4201 caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca
     4261 taccgatgtc gatgaaagcc tgaaaggaca agggattggt aaacagctgg ttgcgaaagt
     4321 cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca
     4381 cgaatttgat aaaacacggg agtatgatga tattcgcagt tgatgggaga gtacagagtc
     4441 acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg gtaggagatg
     4501 cacaaagaat ggtcagagag agcgtttttt tgtcccaagt catccccttt actgagcaaa
     4561 aaaaaaagaa tatctcctat atgagaatca tcattcgggg ttaataagtt ttgcgtcccc
     4621 agagcgttca atattgatag gagtcatatt atggaaggta aaaacaagtt caatacttat
     4681 gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg
     4741 atgtatgata tgaatttctc ctctatcgtg gctgatgaat atgggatacc acgacaattg
     4801 aatgaaaact ccttcgcaat aacgacatcg ttagccgcaa gtgaaatcga agatttaatc
     4861 aggctcaaat gcttagactt accggatatt gattttgacc tcagcattat gacagttgat
     4921 gactatttcc gtcagtttta caagtagcca ggaaggataa gaagaacata aaaaatggca
     4981 ctattctcta aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc
     5041 tggtttatct cacatgagaa aacacatatt cgtttactta gtgcatttct ggtcggaata
     5101 acctggccaa tgagtttgcc tgtggcatta cttttttctc tcttttagga gctgtagttg
     5161 agtggacccg ctggttatct tgaaacgaag cgtctctcct caaaactgga tttgcggaat
     5221 gacgaacgaa gcagtcgcct gactgcttcg ttcattaaag tgaaatttat ttctgtgggc
     5281 gcatcgccgg gaagagaata acgtcgcgaa tagtatgact gtttgtaaac agcataatca
     5341 ttcggtcgat accaataccc agaccagcgg ttggcggcag accatattcc agcgcagtca
     5401 cgtaatcttc gtcatagaac atggcttcgt cgtcacctgc agctttagcg ttaacctgtt
     5461 cctggaaacg ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccaattt
     5521 cacgaccacc gatgaagaat tcaaaacggt cggtgatttc cgggttaaca tcattacggc
     5581 gcgccagcgg ggacacttct gccggatatt ccgtaataaa ggttggctga atcagatgtg
     5641 cttctgccac ttcatcaaag atctccgtaa caatacgccc cagtccccag cttttctcta
     5701 ccgtaatacc gatagattca gctaatgctt tagcagcatc aaaattatcc agatccgcca
     5761 tatcggtttc tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt
     5821 tgccgaaatc aaacacatgc tcgccataag tgactttagt ggtacccagt acctcttgtg
     5881 ccagggtgcg gaacagtgac tccgtcagtt caatcaaatc atggtaatcc gcatatgcca
     5941 tgtagagttc catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga
     6001 agttacggtt gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca
     6061 gctccggcgc gatacgcagg tacatatcta aatccagagc gttatgatgg gtaataaacg
     6121 ggcgagcaga tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc
     6181 cgcgcgcgac catgaactga cggatagcgg ccagaatttt tgaacggaca acaaacgttt
     6241 gacgggattt atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat
     6301 cctgcagacc atggaattta tctggtaaag gacgtagtgc tttagtcagc aggcgcagct
     6361 cagtacagtg aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg cgggcaccga
     6421 taatgtcacc cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat
     6481 ctcttgcaac gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt
     6541 tccccataat acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt
     6601 ccagttcctg gttatccttc gcatcaaact cttcgtgcaa ctggtcagag gtatggtcgc
     6661 ggcgaaaatc attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc
     6721 gatttctcag ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tctcgttcag
     6781 acattttggt tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta
     6841 cactgtatta tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta
     6901 cctcaagttg atatcactaa taaataacca tcaaaatggt ttaaatgact tattaatctc
     6961 gatttactag tgtgataaac tgaaagttaa tttattcata accaattgtt tttacgacag
     7021 ttaatcgttg ctctcctgta tcatattcgt cggcgtgcta aacagaaaac gggtagcaaa
     7081 ggccaatacc acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa
     7141 tcgctggtaa gcggcaattg ccataccgct aatttgcgac tctgtcgtct gctgtgccac
     7201 gacgcctgcc agccagttgg cgaccgcgcc tgttgccagc atataaatac cggttaatac
     7261 gccagacatt ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttcagc
     7321 aaagcccatt agcgccagcc cggatatcat cacgcccatt gacgcttgac cgccagccgc
     7381 tgcatgtcgg gcatcaaatg ccagcaacat aaagccacaa gccatcagca gtaagccaaa
     7441 ggcaaacttc agccagacgc gcaatgctga attgccgcgg ctttctggcg acgccagcca
     7501 ggccagaaca accccagcga gcatcaccgc aatagcattc accgactgga atagtgctgt
     7561 aggtacttca atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt
     7621 actgccgccc tgttgtgcca gtacccagaa caacgtcccg acaaacatca acaatacaat
     7681 ttgccaaaga gcacggcggt gttcggggaa tttgatcatc atgcgggcaa tgatttgtgc
     7741 tgcaatgagg caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt
     7801 aaaaaatact ggggctaaac agagcatcac cactaaccag ctccatactg gtaaggcaaa
     7861 tttgacgcta gtgagcgctt ttttatccat actacgcgtg gactggaaat gacgatgacc
     7921 acttaagaaa atcaacaaac cgataaacat gccgcctccc gcaagggcaa agccaacatg
     7981 ccatccatac cactgagcag ccaggccgca ggcgatgggc gcggcgatag aaccgatatt
     8041 gcccgcagca tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtagag
     8101 ctcgccaagc aaacagctga tgtttgattt gaataaaccg tagccacaaa taatgattgc
     8161 cagcgccaga tacaggctaa aggttgaatt tgtatcaatg cccagcacca catggccaag
     8221 ggtcattaac agcgcgccgg caatcactgc agtgcggttg ccgagcaggc ggtcggcaag
     8281 ccagccgccg agaatagggg taacgtaaac cagagaagca tatgcgctga acaggctgat
     8341 ggcatggtta tcatcaaaac caagctgatg ggtgagatag agaatgagta aggcacgcat
     8401 gccgtaaaaa ctgaagtact cccagatttg gatcgccacg atatagtata tcgcgcgcgg
     8461 ctgtgagggt gttttcatgt gttctcctta tgagcaaaaa agggaagtgg caagccactt
     8521 cccttgtacg agctaattat tttttgcttt cttctttcaa taccttaacg gtatagcggc
     8581 catcagcctg acggtatgca ccgtgaatat cggtttcaaa gcccggatag tgagcgccga
     8641 tttcacacag catctgcagg aactccagaa ccggacggct ttcttcggtg atcatttcac
     8701 ccggcattac cagaggaact cccggcggat acggaaggat catattggcg ttaatacgac
     8761 ctaccatttc gtcgaggtaa acttcttcgg tcataccgtg cagctctttc tggaacgcag
     8821 catacggagt cattaccata gtcggcagaa cttcaaatgc gcgatacatc agatccggca
     8881 gattgtggtg aacaatcagt ttgtggatat tctgagccag ttcctgaata cgcatgtttt
     8941 catagaattc aggatcttca cgatacagag acggcagcat gtttttcaca cgcaggttca
     9001 gatcgaacgc acgtttgaag tcagtcagag cacgcagcag gctcagtgct ttggtcttat
     9061 cgataccgat gctgaacagg aacagcaggt tatacggacc ggttttctca acaacgatgc
     9121 catgttcgtc gaggtatttc gccacgatgc tggccggaat accaaagtcg ctcatggtgc
     9181 cgtctttttc catccccgga gtcagcaggg tgactttgat cgggtcaaga tacatgtgct
     9241 cgttatcgat gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc cagcattcag
     9301 tcgtatcgat atgatccggc tgccatacat caaagaacca gccatcagat tccgttctca
     9361 gacgtttgat ctctttacgg aatttgatcg cacgttcaat ggaaccattg atcagacgtt
     9421 tacctgcatt gcctttcatc atcgctgcag cggtttcagt ggacgccacg ataccgtagt
     9481 gcggagaggt ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg tttacgtcac
     9541 ctttaacgtg gatcatggaa gcctgagaga acgccgccag cagtttgtga gtagactggg
     9601 tttcgtaaat cactttccct tctacacggc caccgctcat accgcattta ccttcgtaaa
     9661 tcggtgagaa gttggtgtaa ggcacccacg cggagtcaaa gtggatggat ttcacatcca
     9721 gtgttttctt gatgaagtcg gtgttgtaca gcagaccatc ataggtagag ttggtaatta
     9781 cagcatgtac cggccaggtt gcgtttggtg tttctttcac gcgcttagca atggtagcgt
     9841 gctggaattc actctgtggg ataccaccaa gaataccgta agcgttacgg gtcgggcgga
     9901 aatagattgg cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg tggcagttac
     9961 ggtcaatcag aatggtgctg cctgccggag cagagtacat accaacaatt ttgttcgcag
    10021 tggaagtacc gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga gcgatatact
    10081 gttctgcttc tttgtgtgga ccactgtgat ccagcagaga acccagttca gatactgaaa
    10141 tggaaatatc agatttcatg gtgttcggac caaagaaatc atagaacagg ctacctaccg
    10201 ggcttttctg gaatgcagta ccgcccatgt gaccaggagt acagaaagta tatttacctt
    10261 cacgaacata tttaaacagt gctttggtca gcggaggcag aatagtgttg atatattcgt
    10321 cagtggtctg cttgatcttg ttagcaatat catcagcagc acccagcgca tattcaaaga
    10381 agctaatctg taaacgcagg tcattcaggc ttacatcgag agtggaatac gtattagcga
    10441 acgcgtacaa cggcaggttc tcgttcattt tgctaatttc ttcgcacagc tcgagattat
    10501 atttatccca gtcaaaaata acaccgcaca gacgcgcatt gttttcgatc agttttaata
    10561 agtcgtcacg gtcgttcggg taaacaatct ggaagttcag acgttcaagc gcgcgatgaa
    10621 gttcacggat gggttcttct ttaaaataaa cccccatgtg attcaatatt gcaataacgt
    10681 tcatagtcat atctccaggt aaaaaaggcc cctcccaaca catgggacaa aatgaaagga
    10741 ggagcctcgg aaaatacttt taattaatgt gcgttagacg ctgtgtggtt atccattgag
    10801 tggctctggc gctcgtgcat tttgcgagcg tagaacatca ggataatcag gctgacgatg
    10861 aaggtacctg ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca gaacacgcaa
    10921 cccagtacag agcagatcag gctgacaaag ttgcggatgt taacgccttc aaaacgaatc
    10981 aggtcaacgc aagagtagaa gtacggcagc atagtcagca gtactgcgat accggtcagt
    11041 tcaccgaaca ggtcagaggc tttaccaccg gcagagttca tcagagtgat gaggatcatc
    11101 agggcagtca ttttcactgc agccagcagc agaccttttt tcggaatacc gttgctgtcg
    11161 acttcaccat aaactttcgg gaagttaccg tcgttagcgg cacgtacacc tgcctggcct
    11221 accaacatca tccaggagcc cagagaagtc agacacgcaa aggcggtgaa tgcagaaacc
    11281 agtggcgcag cccagttacc gaggatagtt gaagcactga ttgcaaacgg agcaccggaa
    11341 gccgccatta cagaagacgg atacataccg gaaagcacct gagtcgcagc gatgtaaaca
    11401 atacctgcta aaccagtacc cagcatggtt gccagcggaa cggtacgttt cgggttttta
    11461 accataccag tacttactgc tgcggattca acacccacga aggcccacag gcagagcaga
    11521 atacttttaa tgatcgcatg accatcagtg gtatccgcag tattccagtt agctgcataa
    11581 gttgctgcat caaaccaatg ccagccaaca atagcagtca tcaccacagg aataagaacc
    11641 agcaccagac caatagtggt taaacggctt acccaggtac cgccgagcat atttacaaag
    11701 gtaaataccc agacgatagc aatacaggcg atacccgccg gaacaggatc atttaatact
    11761 gggaagaagg tggaaagata agatacagcg gtaataccaa ttgccaggtt accaatccag
    11821 ttagcatggt aataaagaac acctgtctga aaaccaaatg caggggaaat ttctccggca
    11881 tacgcaattg ggccaccttg ttgcgggttt tttgttgcca gtcgggcata tacatatgcc
    11941 agcgacattg caccaataat agagataatc caaccccaga tagcaatacc accgatactt
    12001 gctaggttcg caggtaataa tgcaataccg ctccccatca tattaccggc aacaacaccg
    12061 gtacaggcaa atagcccgat cttcttggca gaactcatgc tcttctccta atttcatttt
    12121 tgaatttgga gtccgggtca tgatgtataa ctatttcctg accagaccaa actggcgcta
    12181 agattactca cgaaaaaagg attaatccta aagattaggt gaaataacac aaaagtttct
    12241 gtaagtgaga acttgaggtt ttttattaac acatcaggat cgcaagttga tatcatgaaa
    12301 agataaacat tcaatgttta caatgcattg cgtgacattc tctggttaaa tttatgtaat
    12361 aaaaattatg cggcaaataa attgccgcaa catattatac caacgggaac atacaaaaac
    12421 tcaacaacaa atattcccga gcatgaatca acccgggagt tacttattct gaagcgagaa
    12481 atttgtcgag ataaggtaca acataaggaa cagaagtctg gaatatacca ttttcaatcc
    12541 agtaaagggt gtttgcccct gggcgtaaat taaaggcggt gagatatgca tcagctgctt
    12601 cccggttcat ccccttcatt tcataaacct tgccaagcaa cacataattt agccaggaca
    12661 tttcaagatc aatgccggta tttatcgcct gataagactc atctgtttta ccttttacca
    12721 gggcactgac cgcttttatt tgatatataa tggacaggtt gttcaattcc ggcagtgtaa
    12781 caatgttatc tatttctgtg ttcagtgctg ctaattgttt ttcgtctaaa ggatgttgag
    12841 aatggcgcac gatatcaact aatgcttttt ctgctctcgc gtaggtaaat tctggggatg
    12901 attgaacaat ctcacctaat aattcactgg cacgatccaa tgatttatca tcaccatgca
    12961 gtaaataatc atgtgcctga taaaaattag ttaataacgc accacgatgc ggcaaaattt
    13021 gctggagcat ctcctgcatt cgttgtggcc acggttggtt taacgctttt gataaactct
    13081 ggagcaaatc attttgaatc gccagctgat taccgttagt gatgacataa cgtttatcca
    13141 gcatggttga accatctgca ttgtctacca attttaccga cataaagcat tgttgagcac
    13201 ggtattggcg ctgattaaca aacgcaatag ataatgtttt accggaactg ctcggttcat
    13261 caatgttgta gttgattttg tcatgcacca taaaggtgga gaaggtgtta agtgatgtcg
    13321 ccaccaaatc acccacgcct atcgcgtaag agagctgata cggagaactc cagctgttac
    13381 aactcttatt aaccatatta atgtcaatat cgcgtggatt gagcaaaatg cgcgatttac
    13441 tcataggaag acgtgtttca agacttgaaa acgctaccag tgcgacacag atacctaacg
    13501 acaacaggaa aaaaaaccat acccaaaagg tagtgaatcg tttgctttta actggggatt
    13561 gttcaggtgg cgttgtggtg ttttgaatgt taagactgtg ggagggagaa tctgtggcag
    13621 gaaccgcctc tggtataggg ggaggcgaag atagcattat ttcctctccc tcttcttcgc
    13681 tgtaccagat aaccggcacc attaatttat agccgcgctt tggtacagta gcgatataga
    13741 caggactatc ttcatcatta tcttttaatg acttacgtag ttctgagata ctctgcgtca
    13801 caacgtgatt ggtgacaata cttctcttcc agacattatc gataagttca tctctgctaa
    13861 gtacttcgcc actgtgttga gcaaagaaaa ccagaagatc gattaatctc ggctcaaggg
    13921 taagttgacg cccattgcgg ctaatttggt ttatggacgg agtaacaagc cattcgccaa
    13981 cgcgaactac aggttgttgc ataatagaaa ctcattcgaa aagggaatga tgcaatgata
    14041 attgccataa cctatttttt ccatctat
//
LOCUS       DAPQZD010000124        13980 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPQZD010000124 DAPQZD010000000
VERSION     DAPQZD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.126"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(1..105)
                     /locus_tag="RO454_003559"
                     /pseudo
     CDS             complement(1..105)
                     /locus_tag="RO454_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            849..1055
                     /locus_tag="RO454_003560"
     CDS             849..1055
                     /locus_tag="RO454_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0935333.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLNGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1151..1753
                     /locus_tag="RO454_003561"
     CDS             1151..1753
                     /locus_tag="RO454_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935334.1"
                     /translation="MKQQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDNRN"
     gene            2954..3871
                     /locus_tag="RO454_003562"
     CDS             2954..3871
                     /locus_tag="RO454_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935335.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWIRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            3956..4828
                     /locus_tag="RO454_003563"
     CDS             3956..4828
                     /locus_tag="RO454_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDY0935336.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRNRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            5201..8047
                     /gene="ag43"
                     /locus_tag="RO454_003564"
     CDS             5201..8047
                     /gene="ag43"
                     /locus_tag="RO454_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDY0935337.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDADNGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGNADGVVLESGGRLDVLEGHSAQNTLVDDGGILAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGN
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARI
                     RENITLGVQGGYAHSVSGSSAEGYNGQATLNVTF"
     gene            8118..8276
                     /locus_tag="RO454_003565"
     CDS             8118..8276
                     /locus_tag="RO454_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDY0935338.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            8431..9249
                     /locus_tag="RO454_003566"
     CDS             8431..9249
                     /locus_tag="RO454_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDY0935339.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSPGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPTRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            9591..10064
                     /locus_tag="RO454_003567"
     CDS             9591..10064
                     /locus_tag="RO454_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDY0935340.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            10080..10556
                     /locus_tag="RO454_003568"
     CDS             10080..10556
                     /locus_tag="RO454_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDY0935341.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            10619..10840
                     /locus_tag="RO454_003569"
     CDS             10619..10840
                     /locus_tag="RO454_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDY0935342.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            11003..11371
                     /locus_tag="RO454_003570"
     CDS             11003..11371
                     /locus_tag="RO454_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDY0935343.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            11461..11838
                     /locus_tag="RO454_003571"
     CDS             11461..11838
                     /locus_tag="RO454_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDY0935344.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGFSACTHSQLINSIDILRARRATG
                     LMIRDNYRTVNNITLGKHPEAKR"
     gene            11835..12323
                     /locus_tag="RO454_003572"
     CDS             11835..12323
                     /locus_tag="RO454_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935345.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGYS
                     LRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GTGYLLRTDTWSYPVLRLKRLGLSKTFRRLVVTLIRCYGVSLIHLDASAGYLPGLPTF
                     DW"
     gene            12340..12516
                     /locus_tag="RO454_003573"
     CDS             12340..12516
                     /locus_tag="RO454_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDY0935346.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IAQAREGIRTLRQQ"
     gene            12616..12774
                     /locus_tag="RO454_003574"
     CDS             12616..12774
                     /locus_tag="RO454_003574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935347.1"
                     /translation="MMMSDTTLSRPEVVSGHTDVIYSTSVCHILAVRKSTLLPIDTII
                     RHQVSDIS"
     gene            13138..13314
                     /locus_tag="RO454_003575"
                     /pseudo
     CDS             13138..13314
                     /locus_tag="RO454_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3415 a         3633 c         3599 g         3333 t
ORIGIN      
        1 gtcaacggca gagttaaagg cttccagctc cgcaggggta agtttataac gcgtgtggga
       61 aatcactttt tccagcgtct cccgggatga acaacgacgg aactgctgtg gttttaactt
      121 ccattagcct ctcattactt tatgctgcgg ttaacaggat gctgtcagca taccgcacca
      181 gcctggcatt ctggtaccct ccccaaactc attactcccg tgccaccact taaccggtct
      241 ctcagactgt cactcaccag gtcggatacg tttaccgctc agacggatgc ttaatgcact
      301 ctgttcacgt gtgttgcagg tacgttttat atacgcaggt tatacgccag ctatcagaga
      361 tagccctgtg ggtacaacgc aatctgctgc tactgcgttc tcatgcccac tgatcgcact
      421 cacagtgttt atttcacata tatgaactca tgtatttgat ctcatgtgtt tgatctctct
      481 tatatattta taataccaat cagacagtct tatcccacca gtgataactg tcgcctgccc
      541 acaggtcaca ggctgatata ccacaaccgc cttcaaaaac aactctgcaa ttatcagaac
      601 gctagttttc cccttgtctc acgaaagtcc tccctccttt atcgcgtgcg cgtgcggaag
      661 catcgtttcg caacaaccac ccgacatacc ggtacggctc agccaccgca atccccccgt
      721 ttatctcctg acggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
      781 gtgaaggtgg tgcgttgttt tcatcaccaa cgccggttga gattaacaac atccggagga
      841 acattctcat gaccacccca gtttcactga tggatgacca gatggtcgac atggcattta
      901 tcactcaact gaacggcctg acagataagt ggttttacag gctcatcagg gatggggcct
      961 ttccggctcc catcaagctg ggccgcagct cccgctggct gaaaagtgaa gtggaagcct
     1021 ggctacaggc gcgtattgca cagccccgcc cgtaatttct gaccgttatc cgttcacccg
     1081 cagcagacgt aatccccggc ctgccggcgg cattctgctg cctgttatat cccccgtgag
     1141 gaatatgaaa atgaaacaac agtatcagac ccgttacgaa ctgctccacg aaagctacca
     1201 gaaatggctg accggcttca cccggcacgc cgtatcctgg ggcgtgtgtc atccgaatat
     1261 ctactacttc cataatctga cgcccgggtg ggtgtcattc aatggtgaaa aaccggagat
     1321 tgccatcgta ccccagagcc tgcaccggct gatttatggc cctgacaaac gggccacgcc
     1381 gcccctggat gatgatctga tagtgaattt atgcaccagt gaacatctgc tggttcatca
     1441 tccgatgctg gaaggcattc tcctgtctga atgtgaacgt ctgaggcagc gttctctggc
     1501 aaataaactg attagcctct tccgtcagtt tggtggcacg gagttgcgcc tcaaactggt
     1561 ctggctttgc tggcttgatt taatgaccgg aaacagcctt gaagactgga aagagaacct
     1621 gaaacggaaa tcagaaaaag agctggagga atggatcatc aaccgtcaga ggcagagcgc
     1681 agcactgacg gatctgatgg atcagtacgt gctcctggca taccgcacaa cggttgacga
     1741 taaccgcaac tgatgccggt tgcttccgga aggtgtgaca aaatacggaa atgatgatgc
     1801 tcccggcccc gcatgctcac ctgcagagcg tgttcgtcgt catcatactg agccatgacg
     1861 ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca ccaccagagg agccacggac
     1921 agggatgttc tttacagccc gatgcacgcc aggttctcag cagactgaat gtgcaggtgc
     1981 ggttattcac cagaaatggc atgctgcggt acatacccat cctggtgaca atatgtacgt
     2041 ggcgacagcg atcccggcac tgtggaagag actcactcag cctgcaggat attctttccg
     2101 gcatccggga atgtccgcct gaatgcaaac aggatgatat ttgaatccgt aatactgttc
     2161 tttcatcata aataatttat gcagatacag ttaaccgtca cagtagcagg tttgttttcc
     2221 cgggaaataa tatccgtcag actgatgccg gaacatatct gaaacacgtt atgatgtttt
     2281 gttatcagaa atgttgtata ttcttatccg tggcgacagt ttaatgccct ctggtatcac
     2341 ctgataatgc atatcctgcg atgattcgtt atatttacgg gacacgcctc ttttcattgt
     2401 gctccttttc agacttcatt ttccgtaagt gttccccggc ataaaggagg tacctttctg
     2461 gcgtttataa ctctctttat cggtgacgcc ggtaatacag ggaataccac accgacaaac
     2521 gaggtattaa cagacgatat ttcagtgtcc ataatttttc tgtccggtta aataacgtca
     2581 gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc ataccgggtt acaacgtaaa
     2641 aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac atgtttacct ttcaataccc
     2701 cctgtctgca gtctccactc ttccatcctc ttaagctacg tcggccacat gagtgcactt
     2761 ccatgataac aggtattcct gtcagcataa cgaagccggt taccttccct tcctcctgca
     2821 tttcctgtca gaatgactgt acagcgcgca gattgccttt ttattgacca ccttaatata
     2881 ctattataaa cttcttttta tcatgttatt atcaccttat tatgtttaat ttttaagcaa
     2941 ccggagaaag aggatggttc agaaaatttt gtcggataag gttatgaatg aaagaacaaa
     3001 tgcgtattac tcttattatc ttggagagcg taatatctcc gtactgcccc ttaatgttta
     3061 tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac agagaaaatc tgaacatcac
     3121 actttcagat tttgaacttg aacaaataat ttctggtatg cgtctgaagg ccctggcctt
     3181 cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt gaacgcgcat gcttattttc
     3241 ctggtatctg ttgatgcagt ttatccagaa taacagggca aaaataagcg cagacttgct
     3301 tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa gggaatgcct tcccgtcgga
     3361 ctcatcgaca cagttcagac aaatactcag agttctcgat attctttctg ataagaattt
     3421 gcgggatgag tggataatcc agaccaaaga cagatggata cgggcattta aatcaaagag
     3481 tcctttcagt tacctcctcc cggagaacga acatgaatgc atatggacat ggaattattt
     3541 gaagggaaaa aatattgcac tggaaaaact ggccagtttt cctggttctg ctgatattta
     3601 ccacgcaatc cacctttcat ttgacatatg ggttacctgt ccactcacct cacctgacga
     3661 tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg cagcgcaagt acaaaaaaat
     3721 gcaggaagat aaagtcaatg tacagttttt tcttgacgca gaaaccaggg cgcagctcaa
     3781 agaattatcg agggtcagaa gactcagtac cggcgaaatg ctgcatgacc tgattgttga
     3841 agagtataaa aggtaccgac attccagata atttcagaca tatattatct ccgtgagtcc
     3901 cccgccacct ttccggtgtg cgggggtttt gtcttttttc accgggaata catgtatgaa
     3961 tccgtctgat gccattgagg caattgaaaa accgctctcc tccctgcctt actcgctttc
     4021 ccgtcacatc ctggaacatc tgcgcaaact caccagtcac gaacccgtga ttggcattat
     4081 gggtaaaagc ggggccggta aatcctcact ctgtaatgca ctgtttcagg gggaggtcac
     4141 cccggtcagt gatgttcacg ccggcacccg ggaagtacgg cgcttccgtc tgagtggcca
     4201 tggtcacagc atggttatca ctgacctgcc cggggtgggc gagagccgga acagggatgc
     4261 agagtatgaa gccctgtacc gtgacattct gcctgaactt gacctggtac tgtggctgat
     4321 taaagccgat gaccgtgccc tgtctgtgga tgagtatttc tggcgacaca tcctgcaccg
     4381 gggacatcag caggtgctgt ttgtggtgac gcaggccgac aaaacggagc cctgccatga
     4441 atgggatatg gccggcattc agccttctcc cgcacaggca cagaacattc gggagaaaac
     4501 ggaggcggta ttccgtctgt tccggcccgt acatccggtt gtggccgtat cggcccgcac
     4561 cggctgggaa ctggatacgc tggtcagtgc gctcatgaca gcacttcccg accatgccgc
     4621 cagtcccctg atgacccgcc tgcaggacga gctgcgcacg gaatctgtcc ggtctcaggc
     4681 ccgtgaacag tttaccggtg cggtggaccg gatatttgac acggcggaga gcgtctgtat
     4741 tgcctctgtt gcacgcacgg ttctgcgtgc cgtccgtgac acggtggtct ctgttgcccg
     4801 cgctgtatgg aactggattt tcttctgaac actctgccag gggaaatgcc tgattacatt
     4861 gcgttaacca actgaaaaca tgatacaaac actccatccc cctcctgttt ctcttgcgtg
     4921 actgctctgc tgttaataga ataaaacgat cgataaaaca ggtatcgatc gttaccatcg
     4981 atcgtttata gtggttaatg gtctgcgaaa aggcagctga atctcttcat catgcagaac
     5041 ggaattgcac acaacggact gatacttctc tggcggatga gagaggggaa gcatttatgt
     5101 ccgggaaaaa ccactttatc tgaccatgct gttttgtacc tgccggtatc cacttttgtg
     5161 ggtaccggct tttttattca ccctctgtaa ggaaaagctg atgaaacgac atctgaacac
     5221 cagctacagg ctggtatgga atcacattac gggcaccctg gtggtggcct ccgaactggc
     5281 ccgctcacgg ggaaaacgcg ccggtgtggc ggttgcactg tctcttgctg ctgtcacatc
     5341 agtcccggca ctggctgctg acacggttgt acaggcggga gaaaccgtga acgacggaac
     5401 actgacaaat catgacaacc agattgtcct cggtacggcc aacggaatga ccatcagtac
     5461 cgggctggag tatgggccgg ataacgaggc caataccggc ggacaatgga tacaaaatgg
     5521 cggtatcgcc aacaacacta ctgtcaccgg tggtggtctt cagagagtga atgccggagg
     5581 aagcgtttca gacacggtta tcagtgccgg aggaggacag agccttcagg ggcaggcagt
     5641 gaataccact ctgaacggcg gtgagcagtg ggtacatgaa ggcgggattg caacgggtac
     5701 cgtcattaat gagaagggct ggcaggccgt caaatccggc gcaatggcaa ccgacacggt
     5761 tgtgaatacc ggcgcggaag ggggaccaga tgcagacaat ggtgataccg ggcagtttgt
     5821 tcgcggaaat gccgtacgta ccaccatcaa taaaaatggt cgtcagattg tggctgctga
     5881 aggaacagca aataccactg tggtttatgc cggcggcgac cagacggtac acgggcatgc
     5941 gctggatacc acactgaatg gcggttacca gtatgtgcac aacggaggca cagcctctga
     6001 cacggttgta aacagtgacg gctggcagat tgtcaaggaa ggtggtctgg cggatttcac
     6061 caccgttaac cagaaaggca aactgcaggt gaacgccggt ggtacagcca cgaatgtcac
     6121 cctgaagcag ggaggcgcac tggtcaccag tacggcggca accgtcaccg gcagcaaccg
     6181 tctgggcaat ttcactgtgg aaaacggtaa tgctgacggt gttgttctgg agtccggtgg
     6241 tcgcctggat gtactggaag gccattcagc gcagaatacc ctggtggatg acggcggtat
     6301 cctggcagtg tctgccggtg gtaaggcaac agatgtcacc atgacatccg gtggtgccct
     6361 gatagcagac agtggtgcca ctgttgaggg gaccaatgcc agcggtaagt tcagtattga
     6421 tggtatctcc ggtcaggcca gcggcctgct gctggaaaat ggcggcagct ttacggttaa
     6481 tgccggagga caggctggca acaccactgt cggacatcgt ggaacactga cgctggctgc
     6541 cgggggaaat ctgagtggca gaacacagct cagtaaaggt gccagcatgg tactgaatgg
     6601 tgatgtggtc agtaccggcg atattgttaa cgcaggggag attcgctttg ataatcagac
     6661 gacaccggat gccgcgctga gccgtgctgt tgcaaaaggc gactccccgg taacgttcca
     6721 taaactgacc accagtaacc tcaccggtca gggtggcacc atcaatatgc gtgttcgcct
     6781 tgatggcagc aatacctctg accagctggt gattaatggt ggtcaggcaa ccggcaaaac
     6841 ctggcttgcg tttacaaatg tcggaaacag taaccttggg gtggcaacct ccggacaggg
     6901 tatccgggtt gtggatgcac agaatggcgc caccacagaa gaaggtgcgt ttgccctgag
     6961 tcgcccgctt caggccggcg cctttaacta caccctgaac cgtgacagcg atgaagactg
     7021 gtacctgcgc agtgaaaatg cttatcgtgc tgaagtcccc ctgtatacat ccatgctgac
     7081 acaggcaatg gactatgacc ggattctggc aggctcccgc agccatcaga ccggtgtaaa
     7141 cggtgaaaat aacagcgtcc gtctcagcat tcagggcggt catctcggtc acgataacaa
     7201 cggcggtatt gcccgtggag ccacgccgga aagcagcggc agctatggct tcgtccgtct
     7261 ggagggtgac ctgctcagaa cagaggttgc cggtatgtct ctgacgacag gggtgtatgg
     7321 tgccgcaggc cattcttccg ttgatgttaa ggatgatgac ggctcccgcg ccggcacggt
     7381 ccgggatgat gccggcagtc tgggcggata cctgaatctg gtacacacat cctccggcct
     7441 gtgggctgac attgtggccc agggaacccg tcacagcatg aaagcgtcat cggacaataa
     7501 cgacttccgc gcccggggct ggggctggct gggctcactg gaaaccggtc tgcccttcag
     7561 tatcactgac aatctgatgc tggagccaca actgcagtac acctggcagg gactctccct
     7621 ggatgacggc caggataacg ccggttatgt gaagttcggg catggcagtg cacaacatgt
     7681 gcgtgccggt ttccgtctgg gcagccacaa cgatatgagc tttggtgaag gcacctcatc
     7741 ccgtgacacc ctgcgcgaca gtgcaaaaca cagtgtgagt gaactgccgg tgaactggtg
     7801 ggtacagcct tctgttatcc gcaccttcag ctcccggggt gacatgagca tggggacagc
     7861 cgcagccggc agtaacatga cgttctcacc gtcccggaat ggcacgtcac tggacctgca
     7921 ggccggactg gaagcccgta tccgggaaaa tatcaccctg ggcgttcagg gcggttatgc
     7981 ccacagcgtc agcggcagca gcgctgaagg ttataacggt caggccacgc tgaatgtgac
     8041 tttctgataa tcaggtcact ctccttcagg ccccggtcat catgaccggg gctttttact
     8101 ttcagggagc aacagatatg ccgggttgta catcacgttt attgcccgaa ggccctttct
     8161 cacgcgaaca ggctgttgcc gtcaaaacag cttaccgcaa tgtgtttact gaagatgacc
     8221 agggaacata ttcccggctg gttatccgta atgctgaagg tcagttgcgc tggtgatgcc
     8281 ggaacacagc ttaatactca gctcgccagt taagggcttc tcagacaata aacgtcattc
     8341 atttcatctg tcaggccgcg tcttctccgg gagacgtggc tttttcattt attacaccag
     8401 taaatcttaa ccaccgataa ggagcaaatt atgcgactag ccagtcgttt tggttatacg
     8461 aaccagatac gccgtgaccg tccgctgaca cacgaagaac tgatgcacta tgttcccagt
     8521 attttcggtg aagaccggca tacttcccgc agtaaacgat atgcgtacat tcccaccatc
     8581 accgtactgg aaagcctgca gcgggaaggc tttcagccat tcttcgcctg ccagacccgt
     8641 gtgcgcgacc cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga
     8701 gagataaacg gagaacatgt ccctgaaatc attctgctca actcccatga cggtacctcc
     8761 agctaccaga tgctgccggg ttacttcagg ttcgtctgtc agaacggatg cgtctgtggt
     8821 cagtctccgg gggaagtgcg tgttccgcat cggggaaatg tggtggacag agtcattgaa
     8881 ggggcttacg aggtggtggg cgtgtttgac cggattgagg aaaagcgtga tgccatgcag
     8941 tcgctggtcc tgccgccacc gacacgccag gcgctggcac aggcggcact gacttaccgt
     9001 tatggtgacg aacatcagcc agtcaccacc gctgacattc tgacaccacg acgccgggag
     9061 gattacggta aagacctgtg gagcgcatat cagaccatcc aggagaatat gctgaaaggc
     9121 ggaatttccg gccgcagtgc aaaaggaaaa cgtatccaca cccgtgccat tcacaacatc
     9181 gacaccgata ttaagctcaa ccgcgcattg tgggtaatgg cagaaacgct gctggagagc
     9241 ctgcgctgat gcagcatgtc cctgaaggcg tgattcaggc accagaatca cccgacgccc
     9301 cggaggcgct gccggggtgt cgttccattt ctgatgatga cctgtgcgcc tggtgtaccc
     9361 gtctgcttta ccgtcccgga gaaatcagtc tgtgcagact gagtatacag gcaatggcat
     9421 ctggccatcc gtctgtgacc ggaacggtta cgcgtacggc tgtccggaat ttcagccgaa
     9481 tatcattcat ccctgatatt accgggtcat cacggtcagt gatgaacgtg atgttgccag
     9541 ctcccgacct ttactccctc tcatttattc acattacaag gatttcatac atgaaaacat
     9601 tgtctcagaa caccacctct tcggcctgtg caccggagac tgacctgcaa aaactggtag
     9661 ccactcccgt ccctgatgaa cggcgcatca gcttctggcc gcagcacttt ggcctcattc
     9721 cacagtgggt gaccctggag ccccgtatct tcggctggat ggaccgtctg tgtgaagact
     9781 actgcggtgg tatctggaat ctgtacaccc tgaacaacgg cggggcattt atggcacccg
     9841 aaccggatga cgatgatgac gaaacatggg tactgttcaa tgccatgaac ggtaaccgcg
     9901 ctgaaatgag cccggaagcc gccggcattg ctgcctgtct gatgacgtac agtcatcatg
     9961 cctgtcgtac ggagtgttat gccatgacgg tccattatta ccggctgcgg gattacgccc
    10021 tgcagcatcc ggaatgcagc gccattatgc gcatcatcga ctgaatggag agaagtacaa
    10081 tgcaacagct ttcctttctg cccggagaga tgacgcccgg cgagcgcagc ctcattcaac
    10141 gggccctgaa aaccctggac cgccatcttc atgaacccgg cgtggccttc acctccaccc
    10201 gtgcggcacg agaatggctg attctgaaca tggcgggact ggagcgtgaa gagttccggg
    10261 tgctgtatct gaacaaccag aatcagctga ttgccggtga aaccctcttc accggcacca
    10321 tcaaccgcac ggaagtccat ccccgggaag tgattaaacg cgccctgtat cacaatgccg
    10381 ctgctgtggt gctggcacac aatcacccgt ccggtgaagt cacacccagc aaggcagacc
    10441 gccttatcac ggaacgtctg gtacaggcac tgggcctggt ggatatccgg gtgccggacc
    10501 atctgatagt cggtggcagc caggttttct cctttgccga atatggtctg ctttaacccg
    10561 tcacaaccac atcacacctg ttttcacttt tatcttctgt cttcagaggt atcccactat
    10621 gaaaatcatc acccgtggcg aagccatgcg tattcaccgt cagcatccgg catcccgtct
    10681 ttttccgttc tgtaccggca aataccgctg gcacggcagt gctgaagcgt ataccggtcg
    10741 tgaagtgcag gatattcccg gtgtgctggc cgtgtttgca gaacgccgta aggacagttt
    10801 tggtccgtat gtccggctga tgagcgtcac cctgaactga tgttccgtca cccggaaaac
    10861 gagtacgact ggcagagctc agacgccgct gtggatgagg tcctgctcat caacgaatac
    10921 acctttaccg aagccggtct gcgcgccggc tgacccgtca tcgttaaact atcattcacc
    10981 gattttctgt gaggatttta tcgtgtcaga cacactttcc gggacaacgc atcccgatga
    11041 caatgacgac catccctggt ggggactacc ctgcacagtg acgccctgtt tcggcgcccg
    11101 tctggtgcag gagggtaacc ggttgcatta ccttgccgac cgtgccggta tcagaggccg
    11161 gttcagagat gcggatgcgt accctctgga ccaggccttt ccgctgctga tgaaacaact
    11221 gaaattaatg ctcaccagtg gtgaactgaa tccccgccat cagcataccg tgacactgta
    11281 tgcgaagggg ctgacctgtg aagccgacac cctcggctcc tgtggttacg tttatctggc
    11341 tgtttatccg acacctgaaa cgaaaaagta aaccaccaga ataaccttct gctccggcct
    11401 ggtgttttca ccacgccact gtcccatttt ttatctctgc atatcaggaa aatcttcagt
    11461 atgaaaacat tacccgacac gcatgtacgg gaggtatcgt gctgcccgtc tccagtcacc
    11521 atctggcaga cactgctcac ccgactgctg gaccagcact acggccttac gctgaatgac
    11581 acaccgttcg ctgatgaacg tgtgattgag cagcatattg aggcaggcat ttcactgtgt
    11641 gatgcagtga actttctcgt ggaaaaatac gcactggtac gtaccgacct gccgggattc
    11701 agcgcctgta cccactctca gttaataaac agcatcgata tcctccgggc tcgccgggca
    11761 acaggcctga tgatccgcga caattacaga acggtaaata acattaccct ggggaagcat
    11821 ccggaggcga aacgatgaaa ctggccctga cgctggaagc cgacagcgtt aacgtgcagg
    11881 cgctgaacat ggggcgcatt gtcgttgaca ttgatggtgt taatctcgct gaactgatta
    11941 acatggtctg cgataacggc tactcccttc gtgttgttga tgaatctgac cggacctcag
    12001 cagactgcac gccaccattt gttgccctta ccggcatacg ctgcagtacc gcacatatca
    12061 cggaaacgga caacgcctgg ctgtactcgc tgtcacacca gaccagtgac ttcggtgaat
    12121 cagaatggat tcattttacc ggtaccggtt atctgttacg taccgatacg tggtcatacc
    12181 cggttctgcg gcttaagcgc ctggggctgt caaaaacgtt ccgtcgtctg gtcgtcacac
    12241 tcatccggtg ttatggtgtc agtctcattc atctggatgc cagtgccgga tatctgccgg
    12301 gtttacccac tttcgactgg taatccggaa attcacaaca tgaaatcatt aaccacggaa
    12361 acagcgctgg atattctgat tacgtggctg caggacaata tcgactgcga atcgggaatt
    12421 atcttcgaca acgatgagga taaaacagat tcggcagcac tgttgccctg tatcgcacag
    12481 gccagggagg gtatccgtac cctgcgccaa cagtaacttt tgtaccagaa ccagtgaaga
    12541 tcactcaccc gatttcactt ccctgacgtc agcctgaaca cggctggcat tttcatttat
    12601 aaacagaaag gaaaaatgat gatgtcagat accacactct cccgcccgga ggtggtcagc
    12661 gggcatacgg acgttatcta ttccacctca gtctgccaca ttctggctgt acgaaagagt
    12721 acactgttgc caatcgacac aattatccgg catcaagtaa gcgatatcag ttaaccgata
    12781 tagatgggga agatgctttt atccggattg gttctatcgc gatggtgcga gtaccacttg
    12841 atgcaatgga gcctgtcgag gaagatgacg agtaacagag ctcggtatct caatcaggac
    12901 ctgattgtcc tggttcgatg ccgaaaacca gtgatttttc gtgttggtat ctgtgttggt
    12961 ataggatggt gttgtttatt attaacaatt ttaaatcatg aggttaaatc gcatgtttga
    13021 gtccggcctt cgcaccatac cgtatgcaaa tcgagtcgct caagggcgac tttttttgtg
    13081 tcggtgaccc gtcctgaata gctatgacac gttcctgact taaacccgga gaacgtgatg
    13141 atgtctgggt tcaaacgcac tcaacgtgat tatcctctat cctttaaaat agccgttgtt
    13201 gagaaggttg aaaaaggcga gatgacttat aaacaggccg gctgcagtta tggcattcag
    13261 gggcgctcca ccgttcttgt ctggttgcgt aaatatggcc ggcttgactg gagccccgga
    13321 cttcctgacc aggtaaagag gagactgcct gtggctctgg cgactgtccc gctgacaccc
    13381 gagcaaagaa tcagggaact tgaagaacag cttgagcagg cgagccagaa aactgagttt
    13441 tttaattatt tatcaataac ctgttcaatg actaatggtt ataactgcca ccagaatgcg
    13501 caggctgagt ggataaacgg aatcctgaaa atgagttttt actctcgcgt cctgcagagc
    13561 tgacgtaggg ccggtaaatg gtaaaagaat tcgtggcaat ttataaccat gagcggtcac
    13621 atttggcatt gaaatacaaa acgcccgatg aagttcatca accgttttac ggacagaaaa
    13681 ctgtcaactt atatcaggaa cagttaaagc caggtgcgta ttttcatgaa aggagatcac
    13741 tcaataactc accgatctcc ttcacggtaa tacgcaccag gcatgtggat gagcgtagat
    13801 tatgttgcag ttgcggaagg ggttcttaag gggtatcgag ataacgggtt aagctattta
    13861 ttgatatggt aagtaaccat agtattaaaa ttgatatcga tatttaaagt gattataaag
    13921 caagttattc tttatggaat aaatgataac tgtttctttc ggagcaggat aaaattaatt
//
LOCUS       DAPQZD010000125        13882 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPQZD010000125 DAPQZD010000000
VERSION     DAPQZD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.127"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(431..826)
                     /locus_tag="RO454_003576"
     CDS             complement(431..826)
                     /locus_tag="RO454_003576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935348.1"
                     /translation="MITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGIC
                     SRLGAPNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERW
                     FSSRVQKVQGWSWLIYISGSSNALWKGRS"
     gene            1947..2924
                     /locus_tag="RO454_003577"
     CDS             1947..2924
                     /locus_tag="RO454_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDY0935349.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3209..3949)
                     /locus_tag="RO454_003578"
     CDS             complement(3209..3949)
                     /locus_tag="RO454_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDY0935350.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4070..4252)
                     /locus_tag="RO454_003579"
     CDS             complement(4070..4252)
                     /locus_tag="RO454_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDY0935351.1"
                     /translation="MHVAQLPSLCIFLLSLRSSLPWHYPISPKNRKTALSPRHDWLKT
                     GSLLQNVPLRIWFYPQ"
     gene            complement(4595..4902)
                     /locus_tag="RO454_003580"
                     /pseudo
     CDS             complement(4595..4902)
                     /locus_tag="RO454_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461876.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            5072..5769
                     /locus_tag="RO454_003581"
                     /pseudo
     CDS             5072..5769
                     /locus_tag="RO454_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5785..6114
                     /locus_tag="RO454_003582"
                     /pseudo
     CDS             5785..6114
                     /locus_tag="RO454_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(6223..7155)
                     /gene="mmuM"
                     /locus_tag="RO454_003583"
     CDS             complement(6223..7155)
                     /gene="mmuM"
                     /locus_tag="RO454_003583"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HDY0935352.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(7142..8545)
                     /gene="mmuP"
                     /locus_tag="RO454_003584"
     CDS             complement(7142..8545)
                     /gene="mmuP"
                     /locus_tag="RO454_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HDY0935353.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            8753..9769
                     /locus_tag="RO454_003585"
     CDS             8753..9769
                     /locus_tag="RO454_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HDY0935354.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            9997..10314
                     /locus_tag="RO454_003586"
     CDS             9997..10314
                     /locus_tag="RO454_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HDY0935355.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(10264..10677)
                     /locus_tag="RO454_003587"
                     /pseudo
     CDS             complement(10264..10677)
                     /locus_tag="RO454_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(10601..10960)
                     /locus_tag="RO454_003588"
     CDS             complement(10601..10960)
                     /locus_tag="RO454_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HDY0935356.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQVRLGHVVLA
                     GQQAYHESTGNGMGNPW"
     gene            complement(10988..11167)
                     /locus_tag="RO454_003589"
     CDS             complement(10988..11167)
                     /locus_tag="RO454_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDY0935357.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            complement(11172..11552)
                     /locus_tag="RO454_003590"
     CDS             complement(11172..11552)
                     /locus_tag="RO454_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDY0935358.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(11552..11773)
                     /locus_tag="RO454_003591"
     CDS             complement(11552..11773)
                     /locus_tag="RO454_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDY0935359.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            11956..13512
                     /locus_tag="RO454_003592"
     CDS             11956..13512
                     /locus_tag="RO454_003592"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDY0935360.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSRLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSCYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            13509..>13882
                     /locus_tag="RO454_003593"
     CDS             13509..>13882
                     /locus_tag="RO454_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDY0935361.1"
                     /translation="MSELSYLEKLLDGVEVEWKTLEDISIKISSGGTPKTGVSEFYDG
                     DIPWLRTQEVNFCDIWDTEVKITESGVKNSSAKWIPKNCVIVAMYGATVGKIGINKIP
                     MTTNQACANIQLNEEVAHYRYV"
BASE COUNT         3560 a         3651 c         3427 g         3244 t
ORIGIN      
        1 atgcccgttt tgccataaat ctggttcgtt ccatcatgac ttatgttgat gagttgcttt
       61 agttttaata aaaatatgag actcacagcg aacagataga acttcaacga agaagtgaag
      121 tacggccaga atttcgagct ctggccctcc ggaagatgcc ggacagacgc atcatcccgt
      181 cgtggccgct tccatttttg tgtgtactca acctgccgga tcaggatggc ggcaggattt
      241 gcacccgatg ttccagccgc cttgcctctt cctgatcccg gtattccctc gcctttttca
      301 tcatttctgc cgttggctcc tgcagtttgt cgagaaactc cttagatcgt ttagccaccg
      361 actgaaggga gacactgtcc gtcctgccgg tattgcgatt ggtgtaaacc aggcgggtaa
      421 gggggtctgg ttaactcctt cccttccata aagcgtttga agatccagag atatatatca
      481 accagctcca gccctgcact ttttgtaccc gactggaaaa ccagcgttca gccggcatgt
      541 tcgtcacatc catgaccggt aaaccgggac cgtgtaccca cggcatctca cgtatctggt
      601 aatagaactc cctcagctcc cgctgggtgg tgttgaactg tgactgctgg tccacaacga
      661 tgtcggcttt gcgatttggt gctcccaggc gggaacagat accgcgcaat acgaactgaa
      721 atccaaccat attgggcatg atctgcagct tttgtttacc ggttttgcag ttatatccca
      781 gttcgtcaaa gtgggtcatc gcccaggaca acgcatcggt aatcaattca atgtaacggc
      841 ggtcccccag ggtatgaacg cgcgcaagca gctcactgca gacctcactg aacagagatg
      901 aggaccttcc atcatgggct tcgaggcggg cacaccacgc tttttcagcc agttcattat
      961 cgaacaggct tgccagattc agtagcagcg ggtaacgaaa ggggtccagt aggcaaccct
     1021 gggcactgca ggattcatcc cctgatcgaa gaacgaaata atggcgtgat cccgtttaac
     1081 gacctgccag acatcaaacc gtatccggtg cttcttctgc aggacaagca gcgtatcaac
     1141 cacctcatca aggcggtgca tgccaagctc tgcggcgtgc agacgctgta cttgtcaacg
     1201 ccacgatctt tgaccgtctt atatcgacag agttatccgg catggtgtca atccgttcac
     1261 aatttttcca tcggagcagc ctaccaccgg tgataaccag actttgcctg tagtttgtat
     1321 acacaatccc tggcattgaa acacacatat gaagtgtact tcggatatga ccgtacgctc
     1381 aggaagtacg gcagattccg tcaaaaatta cctgtaaaga cagacatcag aaaaacagtg
     1441 ctcgccgcag accggtgacc gggcgaagcc cgcgaaccgg cgaggaagcg caatggatct
     1501 gtagttacag tgagcaggta cgccctgaag ccttcataat cggtagtgta tgctgttttt
     1561 ctggaatgat ttatccacat atccggaggc ccgatccagt aaacagatcc atgaatgatc
     1621 aacaaaggat ccattaaaga tccccatatc tctgcaaacc ttgtcactca tgggccggga
     1681 ctacgatcac ataagctgtg gcatgttact gataaactgt aacatgctaa tgataagctg
     1741 tattcagtaa tccatatact gaagtaagtt aatgacataa actatggtca gtatgccaga
     1801 ctcagttgtt aaatacaggc tgcaggtttt tcttcagtca gttagcggag ttctgacaca
     1861 cgattttctg tttattcttt tactgtccac aggctggagg ctttctggaa aacgaaaatt
     1921 cagacatcaa aaaactgttc ggcgaggtgg ataagtcgtc cggtgagctg gtgacactga
     1981 caccaaacaa taacaacacc gtacaacctg tggcgctgat gcgtctgggc gtctttgtac
     2041 cgacccttaa gtcactgaag aacagtaaaa aaaatacact gtcacgcacc gatgccacgg
     2101 aagaactgac gcgtctttct ctggcccgtg cagaaggatt cgataaggtt gagatcaccg
     2161 gcccccggct ggatatggat aacgatttca agacctgggt ggggatcatt cattcctttg
     2221 cccgccataa cgtgattggt gacaaagttg aactgccttt tgttgagttt gcaaaactgt
     2281 gtggtatacc ttcaagccag tcatcccgca ggctgcgtga gcgcatcagc ccttccctga
     2341 agcgcattgc cggtaccgtg atctcgtttt cccgtaccga tgagaagcac acccgggaat
     2401 acatcaccca tctggtacag tcagcctact acgatactga acgggatatt gttcagttac
     2461 aggctgatcc ccgcctgttt gaactgtacc agtttgacag aaaggtcctt cttcagctta
     2521 aggcgattaa tgccctgaag cgacgggagt ccgcccaggc actctacacc tttatagaga
     2581 gcctgccccg ggatccggca ccgatatcgc tggcgcggct gcgtgcacgc ctcaatctga
     2641 agtctcctgt attttcccag aaccagacgg tcagacgggc aatggagcag ctgcgcgaga
     2701 ttggatatct tgattacacg gagatccagc gggggcggac aaaactcttc tgcattcact
     2761 accggcgtcc ccggttaaaa gcgccgaatg atgagagtaa ggaaaatccg ttgccacctt
     2821 cacctgcgga aaaagtcagt ccggagatgg cggagaaact tgccctgctt gaaaaactgg
     2881 gcatcacgct ggatgacctg gaaaaactct tcaaatcccg ctgaacataa actgtagtca
     2941 gtgaagagcg ctcctttact gactacagct tatattaacc ggtgcagtga gtggtctgct
     3001 cactgcagtt tatattctgt ttcctgcagt gctgtctgta gctgagctgc catctgcctg
     3061 tcccttacgt aagtgatccc gtaacctgat gctgaggcat tgctcccttc ataaaacatg
     3121 acttactcac tacagcttat atacatactc cagcttatgt tatgtctgtt ctgctgacca
     3181 cagcttatat aaggaagcgc gtatgccgtc acctcagggg aggcagtgta cgcaggatct
     3241 ccgcagcatc ccgcccgtca ccggtgaaag gcactgccag cgtggcagcc atatccagtg
     3301 caaacactct ggtataaacc tccatcgaac gtggatccct gtgaccagcc agtgcctgga
     3361 tgactttccg gggctggcgg tgatagagca tgtgcatgat atagctgtgc cggaaggtgt
     3421 gtggtgtgac cggaatcgaa aagtgtaccc cgtcagcttc ggcccgtctg acagcctgct
     3481 tcagccagtt gcgcatggtt tcgtcggtca cggcccataa tggttcacga cgacggggcc
     3541 gggtggtgat catccagctt tccatctgcc tgacatagct tatatctgtc agcggaacca
     3601 ggcgcacttc atcttttggc gggcgtccgc gtcgcgcacg cactttttcg gacaggatcc
     3661 gcacaaacgg tcttactcca tccaggtcaa atgattccgg tgtcagcatc cgggcttcgc
     3721 caatacgcat tccggtattc cagagggtgg cgaacagcat atggtgacgc tgatccggca
     3781 tatagaaaag aagggcactc acttccgggg ccagcaggta ggccggtgtg gcccccgtgg
     3841 aagtggccat tcttctcagg gagagcgctg ttgcaaaatc cacccccggc gcaatgggta
     3901 acagggagac gcgttctggt gaattctgca ggggaatgac attgttcatg agttattttt
     3961 cacgtagcac acgcactgtt tatggataca ggtattctga cctgaacgcc cggatccttc
     4021 cacgacaata aatcgcagcg cgcggatcct tttgtttgct tatcacgcat tattgcgggt
     4081 aaaaccaaat acgtaaaggt acgttttgca gcagggatcc cgtcttaagc cagtcgtggc
     4141 gcgggctgag ggctgtttta cggtttttgg gagaaatggg gtaatgccag ggcaaagatg
     4201 atcgcaagga aaggagaaaa atacacagag aggggagttg cgctacgtga attctggcta
     4261 ggtacacccc tgtaaccctg ccagttttca cgcaacgcaa tgtggtggct gattactgtc
     4321 agttgaaatg acggctggca tcctgatgct gacttaatat acgaataata acaatttcag
     4381 tatcagtctg cagaaaataa accatatgcc gttcaacggg tagtgcgaat gtgtattccc
     4441 cagttcagga gggggagtac cgatattatg attactcata acctgaaaaa tgtcggatat
     4501 ctgattaata tatttatcag cctactcttc accgaaatgg tggtacccgt aatgctagat
     4561 atcctccaga tcctgactgg ctttaggtgt gagtttaacg gttttcaccg gaagccctcg
     4621 cgccagcttt cacttttttc agaaacgcat ttttttccca cgacacaggt tcaccgctgc
     4681 tcaagccttc tgccagtaaa tcccgcagga cacctggaga cgtgactctg cctgtttctc
     4741 tcgaaggaga cgaagtgact cgcggattac ctcactctgt gtacgataat cacctgattc
     4801 tatgagagat tctatgtact cccagagttc atctccgaga gcaactgtca ttgttctggc
     4861 catatcaagc ctcgtgattg taagtttaaa ttttacatgc agtttattcg atactcaaag
     4921 gtgcttccag tacagaacat ttgggagaat tggggtaatg cctggaacag acagatacgg
     4981 agaaaaaccc tgcagataac cttatacgac aactgaggtg atgctgccaa cttactgatt
     5041 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
     5101 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc actgccggac atcagcgcta
     5161 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
     5221 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaaccgc
     5281 ccgcattatg ggcgttggcc tcaacacgat tttccgccat ttaaaaaact caggccgcag
     5341 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
     5401 cagtggggat acgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagg
     5461 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
     5521 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
     5581 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt
     5641 gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg
     5701 ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
     5761 tatcaataag ttggagtcat taccgccaac ctgttcccgg caaccttcag cgacggatac
     5821 gttgatatct acggctatca tctgccgcgc ccgctgcact ttggtacaca gggtgaaggg
     5881 atggtcggtg tgcgcccgga agcgatcacg ctcagcgatc gcggcgaaga gagccagcgc
     5941 tgcgtgatcc gccatgtcgc ctatatgggg ccgcagtatg aagtgacggt ggaatggcac
     6001 gggcaggaga tattattgca ggtcaacgct acgcgtctgc aaccggacgt cggcgagcag
     6061 tattatcttg aaatccatcc gtacggcatg tttgttctgg cggatgcggc ataacgtttt
     6121 gtggttttgt aggccggata aggcgcttgt gccgccatcc ggcaatgcct gatgcgctgc
     6181 gcttatcagg cctacaatga acgatgtaga cccgataaac cctcagcttc gcgcttttaa
     6241 cgcggcgata tccgcaggcg tggtgcgaca gcacccgcca atcaaccgtg cgccagcggc
     6301 ctgccactgc ggcagataat ccgccagctg cgcgcaatgt tcaccgtgat gatgccaggt
     6361 tttgctcacg gcatcgtaat gctcgcccga gttcggatac accaccagcg gcagcaccgt
     6421 taaaccgtgt aaatgctgca acgcagcggt ggtgttttcc agcgcaatac agttaatgcc
     6481 tagcgccacc acctgcggat aacccgccag caacgcaacc acgtcacgca gcggcgtacc
     6541 gtcgctcagg tgttcgctgt cgcgcagggt aaatgagaac cacgcccgcg cacgcggata
     6601 tgcggtcaac agctcggcca acgcctcaat ctcggaaaaa ttcggcaggg tttcgcaggc
     6661 cagcagatcg gccccggcat ccagcaaggc ttccacgcgc gggcgatgaa acgcctgaaa
     6721 tgcctcaacg ctacaatgat aatcgccacg gtattcagag ccatccgcca gatacgcgcc
     6781 gtaaggcccg acggatcccg ccaccagaag cgttcccgcc tgcgggttct ctgccagata
     6841 cgcttcacgg gctttacgcg ccagctccac gcttttgcca atcagcgctt tcgactgcgc
     6901 ttcatccaga cctcgcgcgg cgaagcccgc cggcgtcgcc tgatagctgg cagtgatcgc
     6961 gcattgcgcc cccgcccggt agtaatcaag atgcacttcg cggataagct ccgggttttc
     7021 taccagcact ttggctgacc acaggctgtc ggctaagtta caccctcgcg cttccagctc
     7081 cgtcgccatc gcgccatcca gcagcaggat atcctgttta tcaagaagag cgcgtaacgg
     7141 attattctgc gacatgttct ggctcctgtt ttgcgtttcg gggttgagta aggaaataag
     7201 caccatagca caacgcaaca aacggtaacc cgcaccacaa cgcaattctc tgcgctggat
     7261 cgaatgccag cccaacacag gccaccaggc acagcacaaa acctaatact ggcaccagcg
     7321 gataccacgg cgcgcgataa tgtaattcac tcaatgcctt accttgttgc agatgacggc
     7381 gacgaaaaac aaaatgcgag gcgcagatac tcagccacac cgctaccacc gcaaacccgg
     7441 agattgccga cagcgcaaca aataccgtgt ccggggccac cacgctggaa aacagcgcca
     7501 gcacaccacc gagcatactg accgacagcg ccgtcagtgg cacgccgttt ttcgttactc
     7561 gcgcaaaaca ggccggtagc gtacgttcat tcgacaacga ccacagcatg cgcccggagg
     7621 catataaccc ggagttcgct gcagaaagaa tagccgtcag gatcacgaag ttaaaaatat
     7681 cagcggcgta cgggatccct actttctcaa ataccagcac aaacgggctt ttctccacgc
     7741 ccgcctgctg catcgggatc agcgctgcca gcacaaacac ggtgccgata aagaaaataa
     7801 tcagtcgcgc gatggtagta cgaatcgcta ccgggataac tttgcgcggg ttttccgttt
     7861 caccggcggc aatgccgata agttcggtac ccgaaaaagc aaagttcact gccaccatag
     7921 tcatcaaaat cggtaagcca ccgtgcggga accagccttc tgccgtgata ttactcagcc
     7981 ccggcgcggg cgagccatcc tgcatcggaa taaagccgaa aatcgccgcc ccaccgagga
     8041 tgataaaggc gatgatagtg accactttga ccagcgagaa ccagaactcc ccttcggcaa
     8101 aaaagcgcgt ggagataaca ttcagaccaa aaataatcgc gcagaacacc acgcaccaga
     8161 cccataccgg cacctgtgga aaccagtact gcatacagaa tccagcggcg gtaaagctcg
     8221 aacccagcgc cacggtccag gtcagccagt aaagccaggc cacggtatac ccggtagccg
     8281 gaccaagata gcgcgcggca taaacgtgaa acgctccggt ctccggcatc gcgaccgaca
     8341 gctcgcccag acactgcata accagccaga ccaccagcgc accaatcaga taggccagca
     8401 gcgttcccgc cgctccagtg gtggaaatga tgtacccggt attgaagaat aatcctgtgc
     8461 caatcacgcc gcccaaggaa agcataatca ggtgacgcgt tttcattgtg cgcttcagtg
     8521 gcgcattttg ttgtgttgtt tgcatataac cttctaaacg tatggacatc taaacatcca
     8581 aagaaggttt gttataccgc gtttgttaac agaatgcaaa gtcaaccctg caccagacgc
     8641 agatgcccgc gccgggcgct taatagcggg tggggattaa taatcagata caaaaagggc
     8701 cgccagctca taacgcctcc ggaaggtgcg aacaagtccc tgatatgaga tcatgtttgt
     8761 catctggagc catagaacag ggttcatcat gagtcatcaa cttaccttcg ccgacagtga
     8821 attcagcagt aagcgccgtc agaccagaaa agagattttc ttgtcccgca tggagcagat
     8881 tctgccatgg caaaacatgg tggaagtcat cgagccgttt taccccaagg ctggtaatgg
     8941 ccggcgacct tatccgctgg aaaccatgct acgcattcac tgcatgcagc attggtacaa
     9001 cctgagcgat ggcgcgatgg aagatgctct gtacgaaatc gcctccatgc gtctgtttgc
     9061 ccggttatcc ctggatagcg ccttgccgga ccgcaccacc atcatgaatt tccgccacct
     9121 gctggagcag catcaactgg cccgccaatt gttcaagacc atcagtcgct ggctggccga
     9181 agcaggcgtc atgatgactc aaggcacctt ggtcgatgcc accatcattg aggcacccag
     9241 ctcgaccaag aacaaagagc agcaacgcga tccggagatg catcagacca agaaaggcaa
     9301 tcagtggcac tttggcatga aggcccacat tggtgtcgat gccaagagtg gcctgaccca
     9361 cagcctggtc accaccgcgg ccaacgagca tgacctcaat cagctgggta atctgctgca
     9421 tggagaggag caatttgtct cagccgatgc cggctaccaa ggggcgccac agcgcgagga
     9481 gctggccgag gtggatgtgg actggctgat cgccgagcgc cccggcaagg taagaacctt
     9541 gaaacagcat ccacgcaaga acaaaacggc catcaacatc gaatacatga aagccagcat
     9601 ccgggccaag gtggagcacc catttcgcat catcaagcga cagttcggct tcgtgaaagc
     9661 cagatacaag gggttgctga aaaacgataa ccaactggcg atgttattca cgctggccaa
     9721 cctgtttcgg gcggaccaaa tgatacgtca gtgggagaga tctcactaaa aactggggat
     9781 aacgccttaa atggcgaaga aacggtctaa ataggctgat tcaaggcatt tacgggagaa
     9841 aaaatcggct caaacatgaa gaaatgaaat gactgagtca gccgagaaga atttccccgc
     9901 ttattcgcac cttccctaga acaacaatgc ctcgattggt ttaaagagct aggttaccaa
     9961 taccaaaatg gctatgacat cgcaccagat ggtgatatgc ctgaacgtga aaactatcag
    10021 caagtcacgc tacaggcgcg tttagtctct gccttggaaa ccttaaaccc aaatgtgccc
    10081 agtgacacct taatcgaagt agcgaaaatc gttgccacac cgcaaacgcc tgtactgatt
    10141 aaaaacaaca aactctttca ccaatattta atcgaaggtg tgcctgttga atactcggta
    10201 atggagcaag aacaatgggt gaccaagcat actcatgttc gattaattga ttttgtgttg
    10261 tggtcaacta aaactggcca ccgcgttaga gtttttccag tatcggtttt ctgattcgtt
    10321 tggtggtaac ccaccattat attcgtgcgg tcttagtgcg ctgtaatatc caacgatata
    10381 gtccgttatg gcgtgagctg catcgctgaa acttacatag cccgtcgctg gcacccattc
    10441 gttcttcaga ctcctgaaga agcgctccat tgggctgtta tcccagcagt ttccacgccg
    10501 actcatactc tgcctgatcc ggtatcgcca cagtaactgc cggaactgcc tgctcgtata
    10561 atgactgcct tgatcgctgt ggaacatcac cccgacgggc ttaccacggg tttcccatgc
    10621 catttccagt gctttcatgg taagcctgct gtccggcgag aacgacatgg cccagcctga
    10681 cctgcccctc tttcctgcgc aaccacgcta ccagcgagtc aattccacca atacggcgaa
    10741 acagttcatc gctgttaaaa aacggctgca acgcctcatt tgttgccata gtttgctcgg
    10801 aaacaacgag gccgtcgtcc atgtgctcga ttaactcacg cggcaccggc tccataataa
    10861 atttacggcc agcctccacc agctttctga cttcctgatc cactttgaat gtggcgacgc
    10921 caagctcttt ttgtacaaag ggagtaatta cggctttcac atcacacctt tcatcactga
    10981 ttgggcttta tctgctgccc ggcattctct gtttaagcac aaccatttcc tgacggcata
    11041 acacagcaat agcggtcctg gcaccaattt gcttaccagc caggtattgc tttacctcgc
    11101 ggcgactcac gccatcaaga agcatcttta acgcttcacg ggacaatttg ttgtatttgc
    11161 gtgccattaa tctactccgc agaaccatac aatctacgta acgtgtcggc gacagaagat
    11221 acagatatct ctccggtcgc agcccctacg gtaaggtctg ccagttcagg tgaatcaaat
    11281 acctgcaccc cgttacggcg tagaaataac agcgcactgt ttagcgcggt acgcttattg
    11341 gcatcattga atatatgccc tctcgctgta gccactaggt aggtggcgga gacttcgaaa
    11401 aggtcggtga tctcttcgta ggcaactctg gcctgaactc tcccgataat ggcctctgcc
    11461 ctgcccggat cagacattcc cggcaggccg ccgtagcggt ttatattcgc atcatgaagc
    11521 gcaataagtt cttccggtga tatatgcctc attatcggtt aaccagttcc ttgttggtgg
    11581 agtccagggt gtcaaacagg gatgcaaatt cagcatccag cgccgctttt ttgtaggctt
    11641 cgaaagtagc cttgctgaca attactgctg gctcacggcc tctgcgggtg atttcaacct
    11701 cttccccggc ctcaacattg ttgagcactt cagaaaggtt gccgcgcgcg gtacggaagt
    11761 taatggattg cataaacacc tcgtgtactc gttatgtgta cacaattata aacttcacag
    11821 gcataaagca ccagcacttt gcggcttaac aaacctctag gcaggtcatt cgtagcctaa
    11881 tgtccgaact gctaaagcat ccaagttgct gtagaatcac cgccaattac ataagcctga
    11941 aataagtgga tgaaaatgac aagtattcaa caacgtgcag agcttcatcg tcaaatctgg
    12001 caaattgcta acgatgtcag gggttcggtc gatggatggg attttaagca atacgttctg
    12061 ggcgcacttt tctaccgttt tatcagcgaa aatttttcca gttatattga agccggtgat
    12121 gacagtatct gttatgcgaa actggatgac agcgtaatta ctgatgacat taaagacgat
    12181 gccatcaaaa ccaaaggcta cttcatctac ccaagccagc ttttctgcaa cgtagctgcg
    12241 aaagcaaata ccaatgacag attgaatgca gatttaaaca gcatcttcgt tgctatcgaa
    12301 agttctgctt acggttatcc ttcagaagct gacatcaaag gtttgtttgc tgatttcgat
    12361 accaccagta accgcctggg taacaccgtt aaagataaaa atgcccgcct ggctgcggtt
    12421 ctgaaagggg ttgaagggtt aaaacttggt gacttcaacg aacatcagat tgacctgttc
    12481 ggtgatgcct atgagttcct gatttctaac tatgcggcaa atgccggtaa gtcaggcggt
    12541 gagttcttta caccgcagca cgtctccagg ctgattgcac aactggctat gcacgggcag
    12601 accagcgtta acaaaatcta cgacccggca gcgggttccg gctcactgtt gttgcaggcg
    12661 aaaaaacagt ttgataacca tatcatcgaa gaaggttttt ttggtcagga aatcaaccat
    12721 acgacctata acctggcgcg tatgaacatg tttttgcaca acatcaacta cgacaagttt
    12781 gatatcaagc tgggcaatac gctgactgaa ccgcacttca gagatgaaaa accgtttgat
    12841 gccatcgttt ctaacccgcc gtattcggtg aaatggattg gcagcgatga cccgacgctg
    12901 attaacgatg aacgttttgc cccggctggc gttctggccc ccaaatccaa agctgacttt
    12961 gcgtttgtat tacatgcgct gaactatctt tctgccaaag gtcgtgctgc gattgtctgc
    13021 ttcccgggca ttttttaccg tggcggcgcg gagcagaaaa tccgtcagta tctggttgac
    13081 aataactatg tcgaaaccgt gatttcactc gcaccgaacc tgttctttgg caccaccatt
    13141 gccgtcaata ttctggtgtt gtctaaacat aaaacggata ccaaagttca gtttattgat
    13201 gccagcgaac tattcaaaaa agagaccaac aacaatatcc tgaccgatgc ccatatcgaa
    13261 cagattatgc aggtatttgc cagcaaggaa gatgttgctc atctggcgaa atctgtcgcg
    13321 tttgagaccg ttgtcgctaa tgactataac ctgtcggtga gctgctatgt agaagcgaaa
    13381 gataaccgcg aaattatcaa tattgctgag ctaaatgcag agcttaaaac tacggtcagc
    13441 aaaatcgacc agttgcgtaa agatattgat gcgattgtgg ctgaaattga aggctgcgag
    13501 gtgcagaaat gagcgagttg agttatctgg aaaaattgct ggatggggtt gaggttgagt
    13561 ggaaaaccct tgaggatatt tctataaaga tatcatctgg aggaacccct aaaactggcg
    13621 tatcagaatt ttatgatggc gacattcctt ggttgagaac tcaagaagta aacttttgtg
    13681 atatatggga taccgaggta aaaataactg agtctggagt taaaaactct agtgcaaaat
    13741 ggataccaaa gaattgtgtc attgttgcta tgtatggggc aactgtgggg aaaatcggca
    13801 taaacaaaat tccaatgaca acaaatcaag cgtgcgcaaa tattcaactt aatgaagaag
    13861 ttgctcatta tagatatgta tt
//
LOCUS       DAPQZD010000126        13628 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPQZD010000126 DAPQZD010000000
VERSION     DAPQZD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.128"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1348
                     /gene="chbC"
                     /locus_tag="RO454_003594"
     CDS             <1..1348
                     /gene="chbC"
                     /locus_tag="RO454_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDY0935362.1"
                     /translation="IASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLI
                     NNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMAL
                     AEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFT
                     FIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTI
                     STPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSV
                     EAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGI
                     FQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGL
                     GAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1399..1749
                     /gene="chbA"
                     /locus_tag="RO454_003595"
     CDS             1399..1749
                     /gene="chbA"
                     /locus_tag="RO454_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDY0935363.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1757..2599
                     /gene="chbR"
                     /locus_tag="RO454_003596"
     CDS             1757..2599
                     /gene="chbR"
                     /locus_tag="RO454_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDY0935364.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2702..4054
                     /gene="celF"
                     /locus_tag="RO454_003597"
                     /gene_synonym="chbF"
     CDS             2702..4054
                     /gene="celF"
                     /locus_tag="RO454_003597"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDY0935365.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4067..4825
                     /gene="chbG"
                     /locus_tag="RO454_003598"
     CDS             4067..4825
                     /gene="chbG"
                     /locus_tag="RO454_003598"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDY0935366.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4872..7133)
                     /gene="katE"
                     /locus_tag="RO454_003599"
     CDS             complement(4872..7133)
                     /gene="katE"
                     /locus_tag="RO454_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDY0935367.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            7316..7579
                     /gene="cedA"
                     /locus_tag="RO454_003600"
     CDS             7316..7579
                     /gene="cedA"
                     /locus_tag="RO454_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDY0935368.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7626..9017)
                     /gene="tcyP"
                     /locus_tag="RO454_003601"
     CDS             complement(7626..9017)
                     /gene="tcyP"
                     /locus_tag="RO454_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDY0935369.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9150..9740)
                     /locus_tag="RO454_003602"
     CDS             complement(9150..9740)
                     /locus_tag="RO454_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDY0935370.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9904..10572)
                     /gene="hxpB"
                     /locus_tag="RO454_003603"
     CDS             complement(9904..10572)
                     /gene="hxpB"
                     /locus_tag="RO454_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDY0935371.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10719..11255
                     /locus_tag="RO454_003604"
     CDS             10719..11255
                     /locus_tag="RO454_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDY0935372.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11296..12156)
                     /locus_tag="RO454_003605"
     CDS             complement(11296..12156)
                     /locus_tag="RO454_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDY0935373.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12262..12552)
                     /gene="ghoS"
                     /locus_tag="RO454_003606"
     CDS             complement(12262..12552)
                     /gene="ghoS"
                     /locus_tag="RO454_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDY0935374.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            complement(12653..13582)
                     /gene="pfkB"
                     /locus_tag="RO454_003607"
     CDS             complement(12653..13582)
                     /gene="pfkB"
                     /locus_tag="RO454_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDY0935375.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3419 a         3222 c         3486 g         3501 t
ORIGIN      
        1 tattgcatcg cttgaaaagg tactcctccc ttttgcagtt aaaataggaa agcagccaca
       61 cgttaatgca atcaaaaatg gctttattcg cttaatgccg ttaacccttg cgggggccat
      121 gtttgtatta attaacaacg tttttctaag ctttggggag gggtcgtttt tttattcctt
      181 aggtattcgc ctggatgcct caaccattga aacacttaat ggtctgaaag gtattggcgg
      241 caacgtatat aacggaacat tagggataat gtctttaatg gcaccgttct ttattggcat
      301 ggcgctggca gaagagcgta aagtcgatgc gctggcggcc gggttgttat ccgttgcagc
      361 atttatgacc gtcaccccat atagtgtcgg tgaggcctat gcggttggcg caaactggtt
      421 aggtggggcg aatatcatct ccgggattat tattggcctg gtggtggcag aaatgtttac
      481 ctttattgtt catcgtaact gggtcattaa gttacctgat agcgttccga catcggtttc
      541 gcgctctttc tcagcattaa ttcctggttt tattattctt tccgtgatgg ggattattgc
      601 ctgggcgttg aatacctggg gcaccaactt ccatcagatc attatggata cgatctcaac
      661 cccactggca tcgttgggta gcgtggtagg ctgggcctat gtgatctttg ttccactgct
      721 ctggttcttc ggtattcatg gcgcgctggc gctgaccgca ctggacaacg gcattatgac
      781 gccttgggcg ctggaaaata tcgcgaccta tcagcaatat ggttccgtcg aagcggcgct
      841 ggcagccggt aagaccttcc atatctgggc caagccgatg ctggactcct ttattttcct
      901 tggtggcagt ggtgcgactt taggcctgat cctggctatc tttatcgcct ctcgacgtgc
      961 tgattatcgt caggtggcaa aactggcgct gccgtctggc atcttccaga ttaacgaacc
     1021 gattctgttt ggtctgccaa ttatcatgaa cccggtgatg tttatcccgt ttgtactggt
     1081 acaaccgatt ctggcggcaa tcaccctggc agcgtactac atgggcatta ttccaccggt
     1141 gaccaatatt gcaccgtgga ccatgccaac cggtctggga gccttcttta acaccaacgg
     1201 tagcgtcgcc gcattgctgg tcgcactctt caaccttggt atcgcaacgt taatttatct
     1261 gccctttgtt gtggtcgcta acaaagcaca aaacgcgatt gataaagaag agagcgaaga
     1321 agatatcgct aacgccctga aattttaatc gatgccggta cgggtaatcg tgccggtaag
     1381 aatagagagg aacgatgtat gatggatctc gataatattc ccgatacgca aacggaagct
     1441 gaagagctgg aagaagtggt gatggggctt atcatcaact ccggacaagc gcgtagcctg
     1501 gcgtatgcgg cactgaaaca ggcgaagcag ggcgattttg ccgcagcaaa agcgatgatg
     1561 gatcagtcac ggatggcatt gaatgaagcg catctggtac agacgaaact gattgaaggc
     1621 gatgctggcg aaggtaagat gaaagtgagt ctggtgctgg tccacgctca ggatcattta
     1681 atgacgtcca tgcttgcgcg tgaactgatt actgaattaa ttgagcttca tgaaaaactg
     1741 aaggcataag gagtcgatga tgcagccagt gattaacgcg ccggaaattg ccactgcccg
     1801 agaacagcag ttgtttaatg gcaaaaactt ccatgtgttt atctataata aaactgagag
     1861 tatcagcgga ctgcatcagc acgactatta tgaatttact ctggtattaa ccgggcgtta
     1921 tttccaggag attaacggta agcgcgtgtt actggaacgg ggcgattttg tttttattcc
     1981 gttaggttcg caccatcaaa gtttttatga gtttggtgcc acgcgtatat tgaacgttgg
     2041 gatcagtaaa cgcttttttg agcagcatta cctgccgttg ttgccttatt gctttgtagc
     2101 ttcgcaggta taccggacca ataacgcttt tctcacctat gtggaaacag tgatttcttc
     2161 attgaatttc cgcgaaacag ggctggaaga gtttgttgag atggttactt tttatgtcat
     2221 taaccgttta cgtcattacc gcgaagaaca ggtgattgat gatataccgc agtggctgaa
     2281 aagtacggta gaaaaaatgc atgataaaga gcagtttagt gaatcggcgc tggagaatat
     2341 ggtgacgttg tcagccaaat cacaggaata tttgacgcga gcgactcaac gatattatgg
     2401 taaaacgcca atgcagatta ttaatgaaat ccgtattaat tttgccaaaa aacaactgga
     2461 aatgaccaac tattcagtga cggatattgc gtttgaggcc ggttatagta gcccgagctt
     2521 gtttattaaa acgtttaaga aattaacgtc ctttacgcct aagagctatc gtaagaaatt
     2581 gactgaattt aatcagtaag tcgttatacc tgacaattca catatctgtc ctgttgctgg
     2641 attatttatg tccgggggca gatatgccag atatcagtat tctgtactga agggagaaat
     2701 tatgagccag aaattaaaag tcgtcactat tggtggcggg agcagctata ccccggagtt
     2761 actggaagga tttattaagc gttatcacga attgccggtc agcgaattat ggctggtgga
     2821 tgtcgaaggt ggtaaagcga aactggatat tatttttgat ctctgccaac ggatgattga
     2881 taacgctggc gtcccgatga agctttataa aacgctggat cgccgcgaag cattgaaaga
     2941 tgctgatttc gttactaccc aactgcgcgt tggccaatta ccggcgcggg aactggatga
     3001 acgtattcca ttaagtcatg gttatcttgg tcaggaaact aacggcgcgg gcggtctgtt
     3061 taaaggtctg cgtaccattc cggtgatttt tgacatcgta aaagatgtcg aagaactatg
     3121 tccgaatgca tgggtgatta acttcactaa cccggcggga atggtcactg aagccgttta
     3181 tcgtcatacc ggatttaaac gctttatcgg cgtgtgtaat attccgatcg gcatgaagat
     3241 gtttattcgc gatgttctga tgctgaaaga cagcgatgat ttatctatcg atctgttcgg
     3301 cctcaaccat atggtgttca ttaaggatgt gctggtaaat ggcaaatcac gctttgccga
     3361 attgcttgat ggtgtggcat ccgggcagtt aaaagcatct ggcgttaaaa atattttcga
     3421 tctgccattt agcgaaggct taattcgttc tctgaatctg ttgccgtgtt cttatttgct
     3481 ttattacttc aagcaaaaag agatgctggc tattgaaatg ggcgaatact acaaaggcgg
     3541 cgcacgagcg caggtcgtac agaaagtcga gaaacaactt tttgagctat ataaaaaccc
     3601 ggagttgaaa gttaagccga aagaactgga acagcgcggt ggggcttatt attctgatgc
     3661 tgcgtgcgaa gtgatcaacg ctatctataa cgacaagcaa gcagaacatt acgttaatat
     3721 cccgcatcat gggcatattg ataatattcc ggcagactgg gcggtagaaa tgacctgtac
     3781 gctggggcgc gatggcgcga cgccacatcc gcgcattacg catttcgatg ataaagtgat
     3841 ggggctgatt cacaccatca aaggcttcga gattgctgcc agcaacgccg cacttagtgg
     3901 agaatttaac gatgtcttac tggcgctaaa ccttagtccg ttggtgcatt ccgatcgcga
     3961 tgctgagctg ctggcacgcg agatgattct ggcgcacgag aaatggctgc caaattttgc
     4021 cgactgcatc gcagagctta aaaaagcaca ttaaccgagg ctgattatgg aacgcttact
     4081 gattgttaat gctgatgatt ttggcttaag caaaggtcag aactacggca ttatcgaggc
     4141 ctgtcgcaat gggattgtca cgtcgacgac ggcgctggtg aatgggcagg ctattgacca
     4201 tgcggtgcag ttgagccgtg atgaaccgag tctggccata gggatgcact ttgtccttac
     4261 tatgggcaag cctctgacag ctatgccggg gttaacccgc gatggtgtgc tgggaaaatg
     4321 gatctggcag ttggcagaag aagatgcttt accgctggaa gaaattactc aggagctcgc
     4381 cagtcagtat ttgcgtttca ttgagctatt tggacgcaaa cctacgcatc ttgatagcca
     4441 tcatcatgtg catatgttcc cgcagatttt cccgattgtg gcaaagtttg cggctgaaga
     4501 ggggattgcg ttgcgcatcg accgtcagtc gctttctaag gatggtgatt taccggctaa
     4561 tctgcgcagt tcgcagggat tcagtagcgc gttttatggt gaagagatta gcgagacact
     4621 gttcctgcaa gtgcttgatg actccagtca tcgcggcgag cgctcgctgg aagtgatgtg
     4681 ccacccggcg tttatcgata atacgattcg ccagagtgct tattgcttcc ctcggttaac
     4741 ggaactggat gtgctgactt cagcgtcgtt gaaatatgcg attgctgagc gtggttatcg
     4801 gttgggtagt tatcatgatg tgtaagtaag gtggtaaggt cactccccag ggggcgtgac
     4861 cttatccata tttacgccgg gattttgtca atcttaggaa tgcgtgacca cacgcggtgt
     4921 gctgtcatca gcgttaacag ttcatccata aaactaccat cggcgcggtc agcttccgca
     4981 atcccttctt caccctggtc agcgaccttg attgttgctt taaacttgcg cgcgtctcct
     5041 gccagcgcaa tcggtttaag gtgtttgtag gcttccatca gatagtagtt ggcatcgccg
     5101 ttgttagcga tatccgcaat attgccgcaa gggacaatga ctgcatcgac cgtcagcgaa
     5161 ggcgcaccgg caaaggtagc agctataggc aacaccgtac cgtcatccgc agtcacttcc
     5221 cccattcgcg agtagagcag tttggcatga acgcctttgg ccttcagcgc cttgagaatg
     5281 gccagaaggt ctgccgatct cacttcatca ttaagcaaaa ttgctaccac gcgacctttc
     5341 acatcaccgt caggaatggc gtacagactt aaggatggat cctttttcag accgttgacg
     5401 ttcggaggcg gggtgatatt caactggtcg tcagtcagtt cgataccgag atttttcgcc
     5461 accgcctggg ccagagtgag atcaatatgc gccagctggt caacaacgcg ctcacgaata
     5521 tacggccgaa ctactttgct taactcaaaa ctgaaaccat cgacaatatg gcgatgctcg
     5581 aatggcgtct gacttagcca gaacagacgc ggatgggaat aatattcgcc aaacgatgga
     5641 ctgcgctcgc gaactttatt gccttccacg cgttcctggt atgattcaaa accgccacgt
     5701 tttggccccg gcggtgtttc gcgcggccag ttatcgttga tcgagttcgg ttcgtaattc
     5761 gccgggttag tgtcgatccc catacgatgc atgccgtcac gctggaaatt atggtaaggg
     5821 caggtcgggc ggttaatcgg aatttcatgg aaattcggcc caccaagacg actgatttgt
     5881 gtatcggtat aagagaacaa acgcccctgc aataacgggt cgttggtgaa atccagaccg
     5941 gggacaatat gccctggatg gaaagccacc tgttcgtttt cagcaaagaa gttatccgga
     6001 ttacgattga gcaccatttt gccgacacgc tgaacgggca ccaattcttc cgggataagt
     6061 ttggttggat cgagaagatc gaagtcgaac ttgaattcgt cttcttcagg aatcaactgg
     6121 aagcccagtt cgtattcagg aaaatcgcct gcttcaatcg cttcccacaa ctcgcggcgg
     6181 tggaagtccg ggtcacgtcc ggttagtttt tgtgcttcat cccaaacgag tgaggcttta
     6241 cctgccagtg gtttccagtg gaaacgtacg aacgttgcct tcccttcggc attaatcagg
     6301 cggaaggtat gaataccgaa gccttccata gtgcggtaac tgcgcgggat accgcgatcc
     6361 gacattgccc acatcacgtt gtgcagagtt tcaggttgca gagaaacata atcccagaaa
     6421 gtatcgtggg cactttgccc ttgtggaact gcccagtgcg gttctggttt taccgcatga
     6481 acaaaatcgg ggaatttatg cgcatcctgg ataaagaaga ttggcgtgtt attgccaacg
     6541 aggtcaaaaa taccctcttc agtatagaac ttggtggcaa agccacggat atcacgcacg
     6601 gtatcggcag agccagcgcc gccctgaacg gtagagaacc taacaaatac tggggtgatt
     6661 ttgttcgtat ctgagaggaa atccgctttg gtgatatcgc ttaagctttt atatggctgg
     6721 aaataaccgt gagctgctga tccgcgtgca tgaacaatac gttccgggat gcgctcatgg
     6781 tcaaagtgag tgattttctc gcgcagaata aaatcttcca gcagagttgg accacggcta
     6841 ccggcacgca gtgagttttg atcgtcggcg atgcgcacgc cctgattagt ggtcagcgca
     6901 taattttcac tgcctttgcg tacgtcttcc agagaattaa gtttttcgtt acgcgtatca
     6961 ggggctttca ggctccctgg ggcggtaggt tgtgcaccag gcggtgttgg ttcagccgct
     7021 ggacgatgag atccgtcttc aggtgccagt gagtccatcc ccggtttcgc ttcgctggaa
     7081 tcgtgtagtg gtgactggtg ctgatgtggg ttcttttcgt tatgttgcga cattgaactc
     7141 gtctccttaa attattactg aaagggccgc tgttttggtc aaaaaaccct ctaactatag
     7201 accaccagcc agatcccgct tcggaaacta ttctggaaac ggtgttatga aatacacgca
     7261 aagcgggacg tacaggggct aaatcggcta aactacagtt tttctgattt ttaatatgcg
     7321 tttagtgaag ccagtaatga agaaaccgct ccgtcagcaa aaccgccaga ttattagcta
     7381 tgtcccacgc acggaacccg cgccgccaga acatgcgata aagatggatt cgtttcgaga
     7441 tgtctggatg ctgcgtggca aatatgttgc gtttgtactg atgggggagt catttctgcg
     7501 ctcaccggcg tttactgtgc ctgaatccgc ccagcgttgg gcaaatcaga tccgccagga
     7561 aaatgaggtc gaagaataaa aaaacacctg tcgggatgac aggcgtttaa cgtaacaaat
     7621 caggattaac ggtgtgccag ttcggcgtcg tcttcgctat ccagaatggc tttatcggtt
     7681 tgcttcagcc actggctggt cagcgtgcca gcggtcatcg agccactaac gtttagcgcc
     7741 gtacggccca tgtcgataag cggttcaacg gagattaaca atgccaccag tgttaccggc
     7801 aggcccatcg caggcagcac aatcagtgcg gcgaaagttg caccaccacc gacaccggca
     7861 acgcctgcgg aactaacggt gacaataccg accagcgtcg caatccacat cgggtccagc
     7921 gggttaatgc ccaccgtagg cgcaaccatc accgccagca ttgccggata caaaccggca
     7981 caaccgttct gaccaatggt cgcaccgaaa gaggcggcga aactggcgat ggattcagga
     8041 acacccagac gacgcgtttg tgcttccaca ttcagtggga tagacgcagc actggaacgg
     8101 ctggtaaagg caaacgtcag tactggccat accttacgga agtacttcag cgggctcacg
     8161 ccattaatgc ccagcagaag gccatgcact gcaaacataa tcagcagacc gaggtaggac
     8221 gcgacaacga aacttcccag tttgatgatg tcttgcaggt tagaacctgc aaccactttg
     8281 gtcatcagcg ccagaacacc gtaaggggtc aactgcatga ccaggcgaac cagtttcatc
     8341 acccagcttt gcagggtatc gatagcggtt aagacgcgtt cacctttcgg cgcatcatcc
     8401 ttcagcagtt tcagcgcagc tacgccgagg aatgcggcaa aaattaccac gctgataatt
     8461 gacgtcggat tggctccggt cagatcggca aacgggtttt tcgggataaa ggacaagacc
     8521 agctgcggaa cgctcaggtc agagacttta ccaacatagt tactttcgat ggcgttcaga
     8581 cgtgcagttt ctgcaccacc ctgaaccaga ccttcagccg tcaaaccaaa caggttggtg
     8641 accagcacac caaccagcgc tgcaatcagc gtggtaaaca aaagcgtacc gatggtcaga
     8701 aaactgattt tgcctaactg agatgcgtta tgcagacggg caaccgcgct cagaatagag
     8761 gcgaagacta gcggcataac gatcatttgc agcagttgaa catagccgtt accaacaatg
     8821 ttaaaccact gtacagaatc tttaagtacc tggctgtcag aaccataaat ggtatgcagg
     8881 gcaaggccaa aaaccacacc catcacaaga cccaccagca cttttttcgc cagactccac
     8941 tgtttgtggc gggtttgagc cagcgcaaac agcagtacaa cgaacaccac gatgttcgca
     9001 attaatggaa agttcatccc cgttctcctg atcttattat ttctatcact gcgtagcagt
     9061 catagtgttg gcgcaagatt aacagaactg tgagctggct cttatattca aatggaattg
     9121 cttattccag aaaggcgatt tttgcctttt tagtattcaa cctgatgctt aataagccga
     9181 tgaaactgga tctgtaaggt attgatcatt tgcgatgacc aacgaacagc gctgttttcg
     9241 ggtaatgaat ggggcatcca taccgaccag acaaataatg ccatgcagat aaaacgttcc
     9301 agttggttgc ccttttgggg aaccaggata ggcaagcgga aacgccagcg gcatggccag
     9361 agcaggggaa caccggcggg tgtcagcata tcggcaagta tgtggctcaa ataacccagc
     9421 accataccct gcaacgcatc agccggaatg aaccagcttt caggaacctt aaggtaaaag
     9481 gttgccagca gcgcaaatac cgccagcagg ctgtgggtaa aaccacgatg cccaaaagcg
     9541 cgggcgatcg gttttgatat ccactttaat cgctgcccaa gaaacgactt tgggtgatcg
     9601 atgtccggta acaaacacgt caggattgcg gaagggacaa tatgccacca gtccccctgt
     9661 gccagcacgg gcgtcagctc ggcatttttg gcaaataccg cacaagcaat agaaaagaga
     9721 aggtgacctt ccgccgtcat gataaaactc actaaactgt cgatacgtac agtatagggt
     9781 ttttatacag tgcacggaag tggtgacggt gtaactcttt ctcttaaatt ggcgattaat
     9841 ccgtcatttg aatgcaaatt cagataaaga accaggggca gtgtgatcac tgccccctgg
     9901 tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc gatgagagtt tgacgtttgc
     9961 taatacaaaa cgtggatcat tttgcgcttc tggcgcagga acgacgatgg aacgcatgcg
    10021 ggctgcttta gaggcgatca tgccatttac cgaatcttcc agtgccacgc aggtcagagg
    10081 gtcaaccccc agttttgctg cgcagtcaag atatacttgc ggatgcggct tgctgtaagg
    10141 cagtttttca gccgaggcga gggcatcgaa actgtcgcgt aaatcaaaca tggtcaatac
    10201 tttttccagc atatgcagcg gtgacgcgga ggccagcccc accaataaac cttgttcttt
    10261 gcataacgca acggcttcac gcacacctgg caataatgga cgtgtctctt caaccagtga
    10321 aatggcacgg gcaataactt gttctactac ttcctgacgg cttggcccat tccatggttg
    10381 ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc
    10441 gttacgacgg gagatatcca cccccaggct tgccatcaca tccagttcgg ctcgatccca
    10501 taaaggttct gagtcgataa gtaatccatc catatcaaaa attgcagcaa gaatctgacg
    10561 cggggttgac attacaacct ctcctttaga gcattatatg taaggtatta agccgtgctt
    10621 atgattaagc gcctatcata tcgcctttaa agaatgggcg aaaacagata tctacaggta
    10681 aactaagtga aacgacgcgt cttaatcaag gagaactcat gacgtatcaa caagctggac
    10741 gcattgctgt tttgaaaagg attttgggtt gggtgatttt tatacctgca ttgatctcta
    10801 cattgatttc tttgctgaaa tttatgaata cccggcagga aaaccaggaa ggcattaatg
    10861 cggtcatgct cgactttact catgtcatga tcgatatgat gcaggcgaat acgccttttt
    10921 tgaatctgtt ctggtacaac tctccgacgc ccaattttaa tggtggcgtg aacgttatgt
    10981 tctgggtgat tttcatcctg atttttgtcg gactggcgtt gcaggattcc ggagcccgga
    11041 tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag
    11101 ccaaagggga agaggggcta acgcgtgaac aaattgagtc acgtattgtt gttccgcacc
    11161 atactatttt cctgcagttt ttctcgctgt atatcctgcc ggttatttgt attgctgcag
    11221 gttatgtgtt cttttctctg cttgggttta tttaatcaaa aggccgcacg gagcggccat
    11281 tatcctcaac ccatatcatg ctgctaataa tctatccagt gactgctgag caataaccaa
    11341 atgctgaccg ccaaataacc ttgcacgatt taacagcgtg tagagttggt aaaccggttg
    11401 acgttcaagg aaatcggcag gtagcgggga tactgactga tagccgtcat agatttgtgg
    11461 cggctgttca gtatgcagcg gtaacatcgc caggtcgcac tctcggtcac cccagtaaca
    11521 ggccgggtcg aaaatgtacg ggccatccgg acccagcgca cagttgccgg accataaatc
    11581 gccgtgcaac agagaaggct gcggttgatg cgaggcaaga cgctgctgaa tatgctcgac
    11641 gatggcatcg atattgccga aagcgatccc tttttctgcc gccagttcca gctgccagcc
    11701 aatccgttgt tcagcaaaaa aagttgacca gcgacgttgc caggtgttgg gctgtggagt
    11761 tgtggagagc gcgttatcga aatcgaggcc aaattgtggt tggtcgctcc attgatgtaa
    11821 acgcgcaatt tgctgaccaa gaataaatgc actatgcgca tccagcgggc gaggtgggag
    11881 ataatccatc accagaaaac tgtagtcacg gtcagcgcca actgcccaaa ccttaggcac
    11941 ggtgacggtt ttactacgcg acagtaactc cagttggtcg gcttcggcgg taaaaccggg
    12001 aagcagttcc ctttcatcac atttgacgaa aaagtcacgt cctgcatagc gcaaatgcca
    12061 tgcggcatgg acttctccgc caggcagttc attacgcagt tctatttcgc cttcacctaa
    12121 ctgctcgctc aaaagacgac tgattgcctg ccacatgatg tctctcccat gttgtctgcc
    12181 agatcataag gttagcgcaa gaatgcggtg taaaaacacg agctaacgca caccacagac
    12241 tgattattgc ccaaaaacca tttattttct gttgctattc cattcctccc aggtcgtgat
    12301 ggtgatttca ggatctttgc cggttgcact gtgggtaagc cccgaaacca gttctctaat
    12361 ttcttcttca ctttgggtac tgataagtcc aaaagtgtta gtccccagct catggatatt
    12421 gccatcatca tctgtcatgg tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc
    12481 gttaatttca gtcaatgtat cctcatgcaa catgacggtt attacataac ggacaagatc
    12541 gccactggcc atagttcacc cctttgttat cacatgactt ttgagcatag tcggagaaac
    12601 gcgttgccga caggttggtg gtgattcccc caatgctggg ggaatgtttt tgttagcggg
    12661 aaaggtaagc gtaaattttt tgcgtatcgt catgggagca caggcgtgtt ccctggttga
    12721 gtgtggctgc actccccgca gctacgccaa aacggaccat ctcttcaaga gaagcatttt
    12781 ctgccagttt aagtgtcatc gcgccgacca tgctgtcacc agcgccaacg gtactctggc
    12841 ttttcaccgg tggtggcacc acctgaatgc agttttcact atcaacgccc agcgcccctt
    12901 gtggacccag ggaaacgaca acccgtttgg ccttgccgct attaacgatt tcctgcgcgg
    12961 ctttgcggac atcgtctggc tgggtgagtt cgcgatttac cagcgcactg agttctttgt
    13021 ggttaggctt aaccaactcg atgttaccaa tcgccagtgc tgcacttaac gcttcgccag
    13081 aactgtcgac gatgcagcgg atcccttgtt tttgcgcagc ggaaatcagt tgggttaatt
    13141 tttccagctt cacacctggc ggcaggcttc cgcttatgac caggatggcc ccggattcaa
    13201 tttccagaac ttgctcttcg agctggcgaa actcatcttc atttaatgcc gcgcctggca
    13261 taacaaaacg atactgctca ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc
    13321 agtctttggc ttctacagta gcgacgggga cattttcatc cgccaacagt gaaaccagat
    13381 gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc agtgcctccc agatgggcaa
    13441 tggcgcgggc gacgttgatg ccgccgcctc cgggttcgaa taccggtgcg gtacagcgca
    13501 gttttccttc gggataaatt tgcggggtaa ttgttgcgct atcgagtgag ggcgcaagtg
    13561 tcaacgtata gatacgtacc atcatttcct cctataggct gatttcagtc tggcaccgaa
    13621 tcagcgga
//
LOCUS       DAPQZD010000127        13598 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPQZD010000127 DAPQZD010000000
VERSION     DAPQZD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.129"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            16..1008
                     /locus_tag="RO454_003608"
     CDS             16..1008
                     /locus_tag="RO454_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDY0935376.1"
                     /translation="MLASTLFLHHLPSFAQSVKFWASLTLPEAQHITRIVSAGAPADL
                     LLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSLEGLMALHPDL
                     VVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLGEEHRAAEQAN
                     LASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLGLHNVAQIADR
                     HGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRILFLSGLPFGWL
                     DAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADYQKLVAS"
     gene            1008..1988
                     /locus_tag="RO454_003609"
     CDS             1008..1988
                     /locus_tag="RO454_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDY0935377.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            1985..2743
                     /locus_tag="RO454_003610"
     CDS             1985..2743
                     /locus_tag="RO454_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0935378.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2753..3565
                     /locus_tag="RO454_003611"
     CDS             2753..3565
                     /locus_tag="RO454_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDY0935379.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3562..4416
                     /locus_tag="RO454_003612"
     CDS             3562..4416
                     /locus_tag="RO454_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDY0935380.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4442..6412
                     /locus_tag="RO454_003613"
     CDS             4442..6412
                     /locus_tag="RO454_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDY0935381.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6462..6716)
                     /locus_tag="RO454_003614"
     CDS             complement(6462..6716)
                     /locus_tag="RO454_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDY0935382.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6917..7651
                     /locus_tag="RO454_003615"
     CDS             6917..7651
                     /locus_tag="RO454_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDY0935383.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7653..8264)
                     /gene="emtA"
                     /locus_tag="RO454_003616"
     CDS             complement(7653..8264)
                     /gene="emtA"
                     /locus_tag="RO454_003616"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDY0935384.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8364..9278
                     /gene="ldcA"
                     /locus_tag="RO454_003617"
     CDS             8364..9278
                     /gene="ldcA"
                     /locus_tag="RO454_003617"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDY0935385.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            9373..11109
                     /locus_tag="RO454_003618"
     CDS             9373..11109
                     /locus_tag="RO454_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDY0935386.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11166..12236)
                     /gene="dadX"
                     /locus_tag="RO454_003619"
     CDS             complement(11166..12236)
                     /gene="dadX"
                     /locus_tag="RO454_003619"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDY0935387.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12246..13544)
                     /gene="dadA"
                     /locus_tag="RO454_003620"
     CDS             complement(12246..13544)
                     /gene="dadA"
                     /locus_tag="RO454_003620"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDY0935388.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEALSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         3140 a         3675 c         3515 g         3268 t
ORIGIN      
        1 cggaggttta cacagattct tgcgtcgaca ttgttcctgc atcatctgcc gtcctttgca
       61 caatcagtca aattctgggc ctcactgacg cttcccgaag cccaacacat tacacgcatc
      121 gtcagcgcag gcgcgcccgc cgatttacta ttgctggctg tcgcgccaga aaaaatggtc
      181 ggtttttcct cttttgattt tgcccgtcag gcattaattc cattgccaga gcacattcgc
      241 cagttcccca ggctgggacg actcgccggg cgcgccagca cactctcgct ggaagggctt
      301 atggcgttac atcccgattt ggtcgttgat tgcggcaata cggatgaaac ctggatctcc
      361 caggcacggc aggttagcga acagacacaa ataccctggt tattgcttaa cgggaaactg
      421 gaacaatcag cagaacagtt aacaacgctt ggcaaaacgt taggcgaaga gcaccgcgcc
      481 gcagaacaag ccaatctcgc cagccgcttc gttggtgaag ctcaggcatt cgccacctca
      541 cccgccgcta atctcagctt ttatgctgcg cgcggtcctc gagggctgga aacgggctta
      601 cagggttcgt tgcataccga ggcggcggaa ttattaggtt tgcacaacgt cgcgcaaata
      661 gccgatcgcc acggtctgac acaggtttcc atggaaaatc tcctgcgctg gcagccggat
      721 attattctgg ttcaggaggc cgttactgca gattttattc gtcgtgatcc tctctggcag
      781 ggcgtgaaag cggttgcgga acaacgcatc ttatttttaa gtggcctgcc ctttggctgg
      841 ctggatgccc cgccgggaat caaccgtctt ctgggattac gcagacttca cgcctggctg
      901 gatcccgcca tcaatcgcca gtttaaaagt gacatgcagc attacgccca actgttctgg
      961 cattgttcac tcagtgacgc cgactatcaa aaattggtgg cgagctaatg agaaccgtaa
     1021 acggatgtat tttgctgctg gcagctatca gtatcacttt tgccgccgta agtggggctt
     1081 atcatctcga tatgcagcaa ctgctggcgc tcattcttcg tcaagaaaat gttcctgtgc
     1141 aggaacaaat tgtcttctgg caaatccgcg tgccgcgtat tcttgctgcg ctgtttctgg
     1201 gcgcggcgct ggcaggggcc ggaaccacct atcagggaat gttccgtaat cccttagtgt
     1261 caccggatat tttaggcgtc tccgccggtg ccggacttgg tgcctgcgcg gcaatcctgt
     1321 ggggattatc cattgtttac atccagctgt acgcattttg cggcgggctg atggtggtcg
     1381 cgggcgtctg gttgattacg cgtcgggtga cccgccacga tccgatctta accctggtgc
     1441 tggtaggcat tgcgttagga acgttgtgcg gagcggggat atcgctaata aaaacgctgg
     1501 ccgatcctta cacccagtta ccgtcaatta ccttctggtt gctggggggg ctgtccaccg
     1561 tgaccttacg cgatctctgc tatgctgcac cgatcattct cataggctcc ttgcccctgt
     1621 tttttctccg ctggcgcatg aatttactca cgctttccga cgacgaagcc cgttcgctgg
     1681 gactgaacgt gacacgtctg cgctttggat tgattgtctg cgccacactc atcaccgcca
     1741 gcacggtagc aatagcaggc ataattggtt gggtcggcct ggtcgttccg cacattgccc
     1801 gcttgttgac cggtcataac catcagcagc tgttgcccat ggcaatgtgt actggcgcga
     1861 ttctgctcct gcttaccgat acgctggcac gcagcattgg aacaacagaa atcccacttg
     1921 gcattctgac cgcttttgtt ggtgcaccgt ttttcctgtt tttgttgtta cgcggaggtc
     1981 gccagtgact ttgcttgccg tccgtcatgc gtctctgggt tactcccgcc atccggtgct
     2041 acgcgacgtg tccttcaccc tgtcgcaagg aacaatttgt tgtctgttag gagccaatgg
     2101 ttgcggcaaa accacactca tgcgctccat tcttggcgtt ataccgttat taaaaggcga
     2161 gatcttactg gatagcctcc ctgtgcagac actcagccat cgccagcgcg cgcaggctat
     2221 tgcctgggtg ccgcaggcgc acgacggcat ttttgctttt agtgtgctgg atatggtgtt
     2281 gatgggcctg gcccccacca taggcgcttt ttccgttccc ggtaagcaag aacgcctgaa
     2341 agcaacggag caactggaaa agctgggcat tttgcatctg gcgacacgac gctggaatac
     2401 gcttagcggc ggtgaacgtc agctggcttt aatcgcccgt gcactcgtcc agcaaccgcg
     2461 cttgttatta ctggatgagc ctgcctccag cctcgatttc ggtcatcaga tccaacttct
     2521 ggacacgctg gcgcagctca aaaataacgg catgacaatg ttaatgtcga ctcatcatcc
     2581 gctccatgca aacgccattg ccgacagcat tatccaggta gagccagatg gacgagtaac
     2641 acaaggctta ccgaccgaac agttaacaac gaaaaaactg gctgccctgt accgggtttc
     2701 tgccgaccag attcaccatc acctctctgc aattaagcac taaggaaaaa tcatgctcat
     2761 tgacgatatt gattttgcag acctttacct tcagcaactc aggctggccc atcgcacaga
     2821 aaaaacgccg gatcactggg atcaacgggc ggaaaaaatg gcggaaaatt gcgcctcgcc
     2881 tacggacagc tacctgcaac aactgacctc aaaaattgac ctgcaaggcg cgcaaacctt
     2941 attcgatatg ggctgcggac cgggtaccgt atcgctggcg ctggcggaca aactcacgac
     3001 gatttatggc gttgattaca gtcaggggat gctgaacgtt gccgcccgtc gtgccgccgc
     3061 gctaaaggca gacaatgtcc actggatcca gcgcgcctgg gaagaggact ggagtgacct
     3121 gccacgctgc gatattgccg tggcttcccg ctccactctg gtggctgata tgcgccaggc
     3181 aatgagcaaa ctgaataatc aggcacggct tcgcgtctat acgactcatc tggtcagcac
     3241 ctcgttcgtc tctccagcca ttcagcgggc ggctggtcgg gaagtgatcg aattacccaa
     3301 ctatatcttt gcactcaacg tcctgtatca gatgggaatt tatgctcacg ttgattttat
     3361 ccgcggacaa aattgccaac aggataacag cacctgggaa cgttttgagc agaacgtcag
     3421 ctggagtctg ggcgcgctta acgacgatga gcgtgagcgg ttatatcgct ggtatcaaca
     3481 acaagatgct cgtgccctcg cccctgccag ccgcgactgg gcgctcatct ggtgggatag
     3541 cgtaccgcag gagacactgc gatgattttc ctttctcagg cacaaatcga tgcgttattg
     3601 ctggaagata tccagggcgg tgacctgacc acgcgagcat taaatattgg acaccagcat
     3661 ggctatatag agttttttct ccgtcagggc ggctgcatca gcggcatttc tgtcgcgtgt
     3721 aagatgttaa ctacgctggg attaactatt gatgacgcgg tcagcgacgg ttcacaagcg
     3781 aacgcgggtc agcggctaat ccgtgcgcaa ggtaatgccg cagcacttca tcagggatgg
     3841 aaggcagtcc agaatgtgct ggagtggagt tgcggtgttt ctgattatct cgctcaaatg
     3901 ctggcgttac ttcgtgaacg ttaccctgat ggcaatatcg cctgcacccg aaaagcaatt
     3961 ccgggcaccc gcttactggc ctcgcaggca attctggctg ccggaggact gattcatcgt
     4021 gccggatgtg cggaaaccat attattgttt gccaaccacc gccattttct tcatgacaat
     4081 caggactggt caggcgcaat caatcagtta cgtcgccacg cacccgagaa gaaaatagtt
     4141 gttgaagccg acacgccgaa agaggcaatc gccgcgttac gcgcgcaacc agacgtactt
     4201 cagctcgaca aatttagtcc gcaacaggca actgaaattg cgcaaatagc accgtcgctg
     4261 gctccccact gcacgctggc tctgaccggc ggaattaatc tgacaacact caaaaattac
     4321 ctcgactgcg gcattcgcct ttttattacc tccgcgcctt attacgcgac accagctgac
     4381 attaaagtca gtctgcaacc cgcagcctct atttaattct ttgttaaacg gaatttattc
     4441 tatgagactc aaaaaacgtt atctctgcac agcattatca cttgccttta cccagcaggc
     4501 cgtagcggct caggagagtg acacgctgac cgtatggtcc agtccggtat catcaacgac
     4561 gaccaccgtt ctcgatcaac ccaccatgaa ggccctggat aaacagaatg tcgctcaggc
     4621 attaagtgtc gtccccggcg tggtgctgca aaagtcaggt agccgcaacg aagaacaggt
     4681 caaagttcgt ggctttgata gtcgtcaggt gccggtctat ttcgacggtg tgcccattta
     4741 tgttccctat gacggcaacc tcgatctggc gcggattctg accaacaatt tgggggcagt
     4801 tgaagtttcc aaagggtatt cgtcgctgct tcaggggcct aatcagatgg gcggagccat
     4861 taatatcacc acccagaagc caacaaaacc tctggaagca agtctgggat atcgccaggg
     4921 atggagccgt agccaggaca atgcctacga tatgcatgct tcatttgccg ccagcagcga
     4981 tctggggtat ttgcaagtca gcggtagcca gctaaagcag gattttctcg gcctgccgca
     5041 tggtgtaaat aatgatattg caggcaaaca cggcaagatg attaattcat cggctgatga
     5101 taaacgcggc attgtgaagc tcggttttac accacgcgaa aacgatgaat acacattgac
     5161 ttacattaag caggatggtg aaaaagataa cccgccatac agtggaaata gtggtcagaa
     5221 atcacgctac tggcagtggc cagagtatga caaagaaagt ttttattatc agggaacgac
     5281 ccaactaaac gatcgtttta ccctgaaaag tcggctgtat cgcgacacct ttgaaaatac
     5341 gctaatgatg tacaactcgc tggctgattt gaaaaataaa aaaggcagct acagccatta
     5401 ttccgattac agcgacggtg ccgggttaca actggcagcc gatgtgcgtg aaaacgatct
     5461 gctgtcgttt gccgttaact ggaaagatga cgtacaccgg gaaaaaggtg cgccgcacgc
     5521 cgcttacgat cgctatgaag atcgtacctg gtcgctcgcc agtgaatatc aatgggctgc
     5581 tgccgataat gtcgatgtcg tggctggaat cagctatgac tggcgcgata gcgtagaagc
     5641 gaaaaaacat gagaaagatg gcagtatcac ccattatgac gacaacaatc agtcagcttt
     5701 taactggcag gtgatgggga aataccactt tgccaatgaa gacacgctgg cgctttcgta
     5761 ctatgaccgc acacgctttc cgacgctgaa agaacgctat accacgtcca aacctgcgta
     5821 taaccagata gcgattgtta acccgcagct caagccggaa cgcgcgcgcg gggtggattt
     5881 aacctggaat ggtgccttca cgcacgactg gggatttgag gtcagcgttt actataaccg
     5941 ggtgagtgat gccatcctct cgcacaatat cgatgccgat accattcaaa atcagaacag
     6001 cggcacggtg gattacagcg gtctggatgc cggtattaag gggaaaatca gcaatatact
     6061 ggatgtagga ttgagctacg ccctgatcca cgctgatgcc aaacgcaaag acatcggcaa
     6121 gataaccgat ctgccaacgc agacaatgac cgcatggatg actctcaaac cgtgggagcc
     6181 gttaagcgta acgctgtcgg aagaggcgcg ttcctccagc tacagcaaca gtgacggttc
     6241 acaaaaagcc gccggttttg cggtgactca cattcgagcc gattacacct taggtcatgg
     6301 cttcagcgtt aatgcgtcgg tcaataacct gtttgatacc aaatatgcct acagtgaagg
     6361 gtttattgaa gaaggtcgca atttctgggc aggtatcgag tatacgttct gatgtgaaag
     6421 cggatagcgg catctgtatc gctatccgcc ttatgaaaat cttattctct tcgcaggagg
     6481 cggaatacgc ccagcaccag gatagcgccc accaccgcca ccaggaagct gtgcaaatta
     6541 aaaccgctga tggagccgcc aatgccaaac atggtcgcca gccagccgcc gaccaccgca
     6601 ccgactatcc caagaataca ggtcaggaaa aatccaccac catctcgccc cggcatgatt
     6661 agcttggcga taatgccggc tatcaggcca aaaataatcc aggcaataat tcccatgttc
     6721 aggccctcgt ttcgatgaat cggacgcaag aaatgcttac gtcaacgctc taagtatagg
     6781 caagggcggg atcgatgctg ggcatatgtc ggctgattta agaacaacat gtaaaaaata
     6841 ttcgcgcatt tgtattaagt tttgttaact gcgaccgata aaccaaagac agtttgtcag
     6901 tcaggagttt ttccgcgtga gtcattacca tgagcagttc ctgaaacaaa atccgttagc
     6961 cgtcctgggc gtgttacgcg atttgaacaa agccgcaatt cctttgcgta tcagctggaa
     7021 tggcgggcag ctgatcagca aaatactggc aataactccg gataaactgg tgctggattt
     7081 cggcagccag gccgaagaca acatcgccgt gctaaaggcg cagcacatta ccattaccgc
     7141 cgaaactcag ggtgcgaaag tcgagtttac tgttgaacaa ctacagcaga gtgaatactt
     7201 gcagcttccg gcatttatta ccgtaccgcc tcccacctta tggtttgtac aacgacgccg
     7261 atatttccgc atcaccgccc cactccatcc gccttatttt tgccagacca aactggcgga
     7321 taacagtacg ttacgtttcc gcctgtatga tttgtcgtta ggcggcatgg gcgcattact
     7381 ggaaacagca aagcctgccg aattacacga aggcatgcgc ttcgctcaga ttgaagtcaa
     7441 catggggcaa tggggtgttt ttcactttga cgcccagtta atctccatca gcgagcgcaa
     7501 agtgattgat ggcaaaaatg aaaccatcac cactccccgt ctgagcttcc gttttcttaa
     7561 cgtcagcccg acggtggagc ggcaattaca gcggattatt ttctctctcg agcgagaagc
     7621 ccggaaaaaa gcggacaatg tgcgtgactg atttacatcg cgtccagtgc ctgctcaagt
     7681 ttgtagatat agcgcggagc ctgcggcgca ggatgatttc gcgctacgtg atcgaggaac
     7741 tcatcagcat ccagatcgtt gattttgctg atcgcctttt tccgatctga cgagaaagtc
     7801 cgtagcagcg cacctgcccc gttagcgtat gacaccacca gcgcatattg cagtaccttc
     7861 ggatcttcaa tgcctgccag cgggccggtt tccagaatat tcaggtaagc cgctcccatt
     7921 gaaatattac gctccggatt tttcagctcg ctggtcgtcg gctcaccgct ccagcccata
     7981 cgacgatata catcacgtcc ggaggttgaa gcttttatct gcatcaaacc aatggcattc
     8041 gatttactca ccgcgttcgg attaccaccc gattcgatag cgataatcgc cgtgatcaat
     8101 tgtggatcga cgccccaggc tgcaccggct ttctggctta ttggcatcca ctgcatcgca
     8161 cgttgcaccg gaactttcgc gttccacggc gggttcgtat agtcatgctt tgatgaacaa
     8221 cccgctaata acacaatcaa aaaggcaaac catctcaatt tcacttcttc tatccttata
     8281 gcctggaatc gccgttgagg tgacaacggc acacctaagc ggcatgatat acatttcgtt
     8341 tctgttgtca gcaaggaatt gccatgtctc tgtttcactt aattgcccca tcgggttact
     8401 gcattaaaca gcacgccgcg ctgcgtggta tccagcgcct taccgacgcg gggcatcagg
     8461 tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg gaaacggagc
     8521 gtctggaaga tctcaattct cttgccagac tgactacccc caacaccatc gtgctggctg
     8581 tacgcggcgg ttacggtgcc agtcgtttac tggcagatat tgactggcag gcactggtgg
     8641 ctcgccaaca acatgacccg ttactcattt gcggacatag cgattttacc gccattcagt
     8701 gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg gtagcaaact
     8761 ttggcgcgga tgaactaaat gcctttaccg agcaccactt ctggctggcg ttacgcaata
     8821 aaacgttcac cattgaatgg caaggggaag gcccgacatg tcagaccgaa ggcacgctgt
     8881 ggggaggcaa tcttgcgatg ctgatttcac tgattggtac accgtggatg ccaaaaattg
     8941 agaacggtat tctggtgctg gaagatatta acgagcaccc tttccgcgtc gaacgcatgc
     9001 tgttgcagct ctatcatgct ggaattttgc cacgtcagaa ggcgattatt ctcggtagct
     9061 ttagcggcag cacacctaat gattatgacg caggttacaa cctggagtca gtgtacgcgt
     9121 ttttgcgttc ccgcctgtcg attccactca ttaccggcct cgattttggt catgaacaac
     9181 gcacggttac gctgccgttg ggcgcacatg ccattctgaa taacaccgag gaaggcactc
     9241 agttgacgat tagcggtcat cctgttctta aaatgtaaaa aaatcacgct tcagggctaa
     9301 gtaaaacgct gtctctgccg ctaatatggt agggtttata tcagagacag cgtaatcagg
     9361 agtaaccgac cgttggatgc cacaacaata attagccttt tcatcttagg atccatcctt
     9421 gtcaccagca gtatattact tagttcattt tcttcccgtc ttggcattcc tattctggtt
     9481 atttttctgg cgatcggcat gttggcgggg gttgatggcg tcggcggtat cccgtttgat
     9541 aattacccct tcgcctacat ggtaagtaac ctggcgctgg cgattatttt gctcgacggc
     9601 gggatgcgca ctcaggccag ttcctttcgt gtggcgttag gaccggcact gtcgctggcg
     9661 acgctgggcg tacttatcac ctctggttta accggcatga tggcggcgtg gctgtttaat
     9721 cttgatttga ttgaaggatt attaatcggc gctatcgtcg gctccaccga tgctgcagcg
     9781 gtcttttctc tgctgggtgg taaggggctt aacgaacgtg ttggctcgac gctggaaatt
     9841 gaatccggca gtaatgatcc aatggcggtc tttctgacga ttactctgat tgcgatgatc
     9901 cagcagcatg aaagcagtgt cagctggatg ttcgtggtcg atattctgca gcaatttggc
     9961 ctcgggattg tcatcgggct tggcggtggt tatttactgc tgcaaatgat taatcgcatc
    10021 gccctgcccg ccggattata tccattgctg gcattaagcg gcggtatttt aatttttgct
    10081 ttaaccacgg cgctggaagg cagcggtatt ctggctgttt atctgtgcgg ttttctgctg
    10141 ggtaatcggc cgattcgcaa ccgctacggc atcctgcaaa atttcgacgg cctcgcctgg
    10201 ctggcgcaaa tcgccatgtt cctggtgctg gggctattgg ttaacccaag cgatctgctg
    10261 cctattgcca ttccggcgct cattttgtcc gcatggatga ttttcttcgc ccgtcctctt
    10321 tcggtatttg ccggattgct accgttccgt ggcttcaatc tgcgtgagcg cgtgtttatc
    10381 agctgggtag gattacgcgg cgcggtgccg attattctgg ctgtgttccc gatgatggcg
    10441 gggctggaga atgcgcgact gttctttaat gtcgccttct ttgtggttct ggtttcactg
    10501 ctattgcagg gaacatcact ctcgtgggcg gcgaaaaaag ccaaagtggt cgttccgcca
    10561 gtgggacgtc cggtgtcacg cgttggcctg gatattcatc cggaaaatcc gtgggagcag
    10621 tttgtttatc aattgagtgc cgataaatgg tgcgtgggcg cggcactgcg tgatttgcat
    10681 atgccaaaag agacgcgtat tgcggcactg tttcgtgata accagttgct tcatcccacc
    10741 ggcagcaccc gactgcgcga aggcgatgtg ttgtgtgtaa ttggtcggga acgcgatctc
    10801 ccggcgctcg gtaaactgtt cagccagtcg ccgccggtcg cgctggatca acgcttcttt
    10861 ggtgacttca ttctcgaagc cagcgccaaa tatgctgatg tggcgctgat atatggtctg
    10921 gaagacgggc gagaatatcg cgataagcag caaacgctgg gtgaaatcgt ccagcagttg
    10981 ttaggcgcgg caccggttgt cggtgaccag gtagagtttg ccgggatgat ctggacggtg
    11041 gccgagaaag aagataatga agtgttgaag attggtgttc gggtagcgga ggaagaagcc
    11101 gaatcttaag tttaatgtgt ttcgtaacgc tggatacgac gctggtcgca tccagcgata
    11161 acaagttaca ccgtcacaac cgggacgcgt aacgccagcg cgcacatcaa ctcatagcct
    11221 accgttccgg cagcggcggc gacatcatca attttgatct ccttgcccca cagctcaact
    11281 ggcgtaccaa tacccgcttg cgggcaaggc gttaaatcga ccgccagcat atccatcgag
    11341 actgtcccca ccgtcatggt gcgaacgccg tccactaaaa caggggtacc ggtaggcgcg
    11401 tggcgcggat aaccgtcggc gtatcctgcg gcgacaatgc cgattcgctg ttcatcgcgc
    11461 gcagtatagc gaccgccgta gcccacacgc tcgcccgctt ttagcgtctg gacaccaata
    11521 atctcactgc ttagcgtcat caccggacgt aatccggtat tggcgatatc acgccactga
    11581 ccggacggcg aagcaccata caaaataatg ccaggccgaa cccagtcaaa atgcgcttcc
    11641 tggtgccaca gagtcgccgc cgaattagac aacgaacgcc gacactccag cccttccgcc
    11701 gcctgctcaa tacgcgccat cgcgctggaa attccatcag gatgttccgc ttcggcaaaa
    11761 tgcgacatca gggtcatttc gccaacattc gccatcgccc gcaactgctg ccagacggta
    11821 agcacgcgat cgggctggaa gcccaaccga ttcatcccac tgttcacttt aagataaata
    11881 tccaacggtg cttttagccg cgcattttgc agtgctttga gctgccagtt gctgtgtacg
    11941 caggtggtca ggcggtgctg gtcataaatc tccagatcct gagcatggaa aaatccttcc
    12001 agcatcagga tcggtccttt ccagccgcgc tcacgtaacg ttattgcctc ttccaggtta
    12061 agcaatgcaa agccatcggt agccccgagc gcgctccaga tacgctcaat accgtgcccg
    12121 taagcgttcg cttttaccac cgaccagacg cgcgcgtgcg gcgcggcctg gcggacaatg
    12181 gacagattct gttttaatgc ctgcagatcg aggctggcct gtatcggacg ggtcatctcg
    12241 tttccttagc tgtgtgcgcc atgtaaatgg cccgggcgtg atggcgtaaa tccacggctg
    12301 tagcgcgcta cgcttagagc ttcatacggg atcgctggcg tgcgccccga gagcagatcg
    12361 cttaacaact gaccggaacc gcaagccatc gtccagccga gcgtgccgtg accggtattc
    12421 aaccacagat ttttaaagcg tgtacgcccg acaaccggcg tgccgtctgg cgtcatcggg
    12481 cgcaggccag tccagaaagc cgcctgatca acatgaccgc cgcgtggata gagatcgcga
    12541 accaccatct ccagcgtttc acgacgcggt tgcaacagct cggtgttaaa accaacaatt
    12601 tccgccattc cgccgacacg aatgcggtta tcgaaacggg taatggcgat tttgtaggtt
    12661 tcatcaagaa tagtggatac cggcgcacca tcttcctgcg caattggaat ggtcagcgag
    12721 tagcctttca gcggatacac cggaatatca acaatgcctt tgagcatcgc cgtcgagtaa
    12781 gaaccaaacg ccatcacata cgcatcggcc ttaatcactt catcaccaca cttcacgccg
    12841 tagatttgct cgccgtcgca aagcagttgg tcaacgggcg tattaaagcg gaatttaacc
    12901 cccgcctgct ccgccatccg cgccagattc tgggtaaaca gctggcagtc tccggtttca
    12961 tcattgggca gttgcaggcc gcccgtcagt ttgtgcgcta cttctgccaa cgcgggctcc
    13021 acttccgcca ggcggctgga ttccagcagc tgatacggta cgccagcatc ttccagcacg
    13081 gcgatatcgc gggtcgcgtt ttcatactgt tgttcggtac ggaacagttg cagcgtccca
    13141 ccctgacgcc cttcatactg aatattggtt tcggcgcgca atgctttcag gcaatcacgg
    13201 ctgtattccg ccagacgtac catccgccct ttgttttcca tgtagtggct ggtgtcgcag
    13261 ttacgtaaca tttgccacat ccatttcaac tggaactgcg taccgtcgag acgaaccgcc
    13321 agcggcgcat ggcgctggaa catccattta atcgctttta aaggcacacc tggtgccgcc
    13381 cacggtgcag catatccggg ggagatttgc ccggcattcg cggcactggt ttccagggcg
    13441 gcccccggct cccgatcaat gacggtgacc tcatgtcctg cctgatttaa gtaccaggcg
    13501 ctggcaacgc ctaccacacc acttcccagt atgacaactc gcatagccac tccgataacg
    13561 gtagatacag taaaagaata ataatctaat tacatctt
//
LOCUS       DAPQZD010000128        13597 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPQZD010000128 DAPQZD010000000
VERSION     DAPQZD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.130"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1227
                     /locus_tag="RO454_003621"
     CDS             <1..1227
                     /locus_tag="RO454_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDY0935389.1"
                     /translation="LIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLA
                     FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDN
                     ILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTK
                     ILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGI
                     EVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFC
                     VMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELN
                     SAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLVMTEADKAL
                     REAKSEGGNKVIIHHI"
     gene            1408..2898
                     /locus_tag="RO454_003622"
     CDS             1408..2898
                     /locus_tag="RO454_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDY0935390.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2941..3444
                     /gene="yeaK"
                     /locus_tag="RO454_003623"
     CDS             2941..3444
                     /gene="yeaK"
                     /locus_tag="RO454_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDY0935391.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3445..3549)
                     /locus_tag="RO454_003624"
     CDS             complement(3445..3549)
                     /locus_tag="RO454_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935392.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3719..4165
                     /locus_tag="RO454_003625"
     CDS             3719..4165
                     /locus_tag="RO454_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDY0935393.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4122..4943)
                     /gene="nimR"
                     /locus_tag="RO454_003626"
     CDS             complement(4122..4943)
                     /gene="nimR"
                     /locus_tag="RO454_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDY0935394.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPGSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5040..6221
                     /gene="nimT"
                     /locus_tag="RO454_003627"
     CDS             5040..6221
                     /gene="nimT"
                     /locus_tag="RO454_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDY0935395.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6276..6623
                     /locus_tag="RO454_003628"
     CDS             6276..6623
                     /locus_tag="RO454_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDY0935396.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6645..6899)
                     /gene="yoaF"
                     /locus_tag="RO454_003629"
     CDS             complement(6645..6899)
                     /gene="yoaF"
                     /locus_tag="RO454_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDY0935397.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7082..8107
                     /gene="dgcP"
                     /locus_tag="RO454_003630"
     CDS             7082..8107
                     /gene="dgcP"
                     /locus_tag="RO454_003630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDY0935398.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            8140..8238
                     /locus_tag="RO454_003631"
     CDS             8140..8238
                     /locus_tag="RO454_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDY0935399.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8241..8315
                     /gene="yoaJ"
                     /locus_tag="RO454_003632"
     CDS             8241..8315
                     /gene="yoaJ"
                     /locus_tag="RO454_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDY0935400.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8374..8622)
                     /locus_tag="RO454_003633"
     CDS             complement(8374..8622)
                     /locus_tag="RO454_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDY0935401.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8770..8952)
                     /locus_tag="RO454_003634"
     CDS             complement(8770..8952)
                     /locus_tag="RO454_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDY0935402.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8956..9315)
                     /gene="yeaR"
                     /locus_tag="RO454_003635"
     CDS             complement(8956..9315)
                     /gene="yeaR"
                     /locus_tag="RO454_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDY0935403.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9488..10126)
                     /gene="leuE"
                     /locus_tag="RO454_003636"
     CDS             complement(9488..10126)
                     /gene="leuE"
                     /locus_tag="RO454_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDY0935404.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10253..10585)
                     /locus_tag="RO454_003637"
     CDS             complement(10253..10585)
                     /locus_tag="RO454_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0935405.1"
                     /translation="MSLTPNALDTTTSKYRFLNDDFILCSFIHSTAWRGIFAFGVFPD
                     HVKIDVARLFPASGHLVQVLPEYYQPANVWAVYVSKLSTSAKVRITVEFLRQYFAEHY
                     PNFSLEHA"
     gene            complement(10669..11784)
                     /gene="rnd"
                     /locus_tag="RO454_003638"
     CDS             complement(10669..11784)
                     /gene="rnd"
                     /locus_tag="RO454_003638"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDY0935406.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11866..13551)
                     /gene="fadD"
                     /locus_tag="RO454_003639"
     CDS             complement(11866..13551)
                     /gene="fadD"
                     /locus_tag="RO454_003639"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDY0935407.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         3428 a         3353 c         3196 g         3620 t
ORIGIN      
        1 ctcatcccac agatgaatta cctgatggtt gttgtggctt tgtttttcct taacgccgtc
       61 atttttcttt tcatgttaat gaagtatttc actaacaaac aaattttacc aacactcatt
      121 ttaagccttg catttttaag tggccttatc tatttagttg aaaccattgt aattatccat
      181 aaaccaatca acggcagtac actgatccag acaaagtcga atgatgtttc tattttctat
      241 attttccgcc aactcagttt catttgttta acctcgctgg cgctcttttg ttatgggaaa
      301 gacaacatcc ttgacaacaa taagaaaaaa actggcattc tgttgctggc gctgattcct
      361 tttttagttt ttccccttct ggcacacaat ctgagcagtt ataacgctga ctattctttg
      421 tatgtcgtcg attactgccc ggacaaccat actgcgacct ggggaatcaa ctatacaaaa
      481 atattggttt gtttgtgggc atttttactg ttctttatta tcatgcgtac acgattagcc
      541 agcgaactat ggccgttaat agcattatta tgtctggcat cgctatgctg caacttactt
      601 ctactgactc tggatgagta taattacacc atctggtata tcagtcgcgg gattgaagtt
      661 tccagtaaac tgtttgttgt gtcttttctg atttataaca tttttcagga actgcaactc
      721 tccagcaaac tggcagttca tgatgtgctg accaatattt ataatcggcg ctactttttc
      781 aacagcgtag agtcattatt gtcgcgacct gttgttaagg acttttgtgt catgctggtt
      841 gatattaatc agttcaaacg catcaatgcc caatggggac atcgtgtggg tgataaagtg
      901 ctggtatcaa ttgtcgatat tatccagcaa agcatccgcc ccgatgatat tttagcgcga
      961 ctggagggtg aggtgtttgg cttgctattt accgaactca atagtgccca ggcaaaaatc
     1021 attgcggaac gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa cagatatgat
     1081 gttcctgaac aaatgaccat cagtattggc acggtttttt caacgggtga cacgcataat
     1141 atctcgcttg tcatgacgga agcagataaa gccttacgcg aagcgaaaag cgaggggggc
     1201 aacaaagtga ttattcatca tatttaagtg caaaaatatt cacagccatg ctttttgcgt
     1261 ggcttttgca tacaatttat tactattacc cctaaatttc ccctccccag cggcgcagag
     1321 atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg ttttctcgtt
     1381 tcactaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct ccggcatttt
     1441 atcgccgcaa gtgtcattgt gctgacatct tccttcctta tttttgaact tgtcgccagc
     1501 gacagagcaa tgagtgccta tctgcgctat atcgtgcaga gagcagactc ttcctttctt
     1561 tatgataagt atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag
     1621 cagtcggaag taccgccaga acaacggcgc gctatctgcg aggcttttga gtctgccaat
     1681 aatacccatg gcttaaacct gactgctcat aaataccagg gcttacgcgg cacactacaa
     1741 accgcatcca ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat
     1801 caggcagtgg aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa
     1861 gagaaatttc actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt
     1921 aatgttgaat actttgcgat gaataactgg tccttcctgc aatcaggaag tattggtatc
     1981 gatcgcaaag atattgaaaa agtatttacc gggcgtaccg tattatcgag catttaccag
     2041 gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag
     2101 ctaaaaggga ttgtgctgct ggatattaac aaaaacaatc tgcggaatat cttttacact
     2161 catgaccgcc ctctcctctg gcgttttctc aatgtcacgc taaccgatac agattcgggg
     2221 cgcgacatta tcatcaacca gagcgaagat aatctgttcc agtatgtcag ttacgtccat
     2281 gacttaccgg gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct
     2341 tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtgcgg
     2401 atgcacttcc gcttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga
     2461 ctctataatc gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa actggtgcaa
     2521 tccggttctt cggtgatgtt tattgctatt gacatggaca agttaaagca aataaatgac
     2581 accctcggtc atcaggaggg ggatttagcg attacgttat tagctcaggc gattaaacag
     2641 tcgattcgta aaagtgatta tgccatccga ctcggtggcg atgaattctg catcattctt
     2701 gtcgattcga cgccgcaaat tgcagcacaa ctgcctgaac gtatcgaaaa acgtctgcaa
     2761 catatcgcgc cgcagaaaga gatcggcttc tcttccggta tttacgcgat gaaagaaaac
     2821 gatacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa taagcagaac
     2881 aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca ggagcatgaa
     2941 atgactgaaa tggctaaagg aagcgtgacg catcagcggt taatcgcttt attatcacaa
     3001 gaaggtgcta acttccgcgt cgttacccat gaagcggtag gtaaatgtga agcagtgtca
     3061 gaaattcgtg gcaccgcttt aggccagggg gctaaggcac tggtatgtaa agtcaaaggc
     3121 aatggcgtaa atcaacatgt tctggcgatc ctcgctgccg atcaacaggc cgatctgagc
     3181 caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc ggaagtcgat
     3241 gaactcacag gctgtgtctt cggcgcgatc ccccctttca gcttccatcc aaaactcaaa
     3301 ctggttgccg accccctact cttcgaacga tttgatgaaa tcgccttcaa tgcaggcatg
     3361 ctggataaat ccgttatttt gaaaaccgcg gattatttgc gtattgcgca accagaactg
     3421 gtcaatttcc gccgcactgc gtaactagcc tgtccgttca ataagcagaa cggacaaaac
     3481 cagcacagca gcaatggcaa aaaacgatga cgtgataatc agtgtctcga cgaacatttg
     3541 atcgttcata gtatgccccc gggagatagc tgttttcacg ttattgttaa cgtacacaaa
     3601 tggcagtttg atgacagttc gtctgatttt ttatgcaaaa aacgtaaaga ttttctactt
     3661 cctgcctgca gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat
     3721 gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa
     3781 caccactgtc gccgtttcaa ttctggtgtt gattattgtc cgcgtcacac cgttaagcac
     3841 ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg
     3901 cgttatggcc cccatcgcca gcgggacgct accgccctca accctgattc actcgtttct
     3961 gaactggaaa tcgctggtgg caattgcagt cggcgttatt gtctcctggc tgggggggcg
     4021 cggcgtgacg ttaatgggca gccagccgca actggtcgcc gggctgctgg tgggcactgt
     4081 tttaggcgtg gcgctgtttc gcggcgtacc ggttggcccg cttattgccg cgggtctggt
     4141 ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc
     4201 aggcctttct taaacatagt aataaaggcc gttgttgaat cataccccag tgtatgagca
     4261 actttctgta cagtatcgcc tttcactaat ccctgaagcg ccataatcag ctgtaactgc
     4321 tgacgccact ggcgaaaact caaccccgtc tctttcacta tcagccgggc aagattgcgt
     4381 tcactcatcg caaaaaagcc agcccattgc ctcaatgagc cccactcgac gggctctttc
     4441 gccatcatct ccaccatcgt gcggatttta ggatgagaag aaccgggcaa gtgcagctgt
     4501 tgttgtggtt gctgagggag ttcatcaaac aggacctgaa ttaatctgcg ggtcatcggc
     4561 tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa ttaattcccg gcataagggc
     4621 gatattttca atgtgcaaca tgttgtcggc attgtcactg cgctgggctc gataaacaga
     4681 aaacagagtt cggcgttagc tgtcacttga ttactatgtt tcaccccgcc cggtatccaa
     4741 accgcatatt gtggcggcac catccacaaa gcattttcta ccgtacaggt aattgcacca
     4801 tgtagtgcca gtatcaactg ccctttgcgg tgctgatgta caggactggt taactcatcc
     4861 gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca
     4921 ttgagattca acctgtgcat cattttgtcc gaatttagcg atattttgtc attttagctt
     4981 gattcaacat aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata
     5041 tgacctgttc aacttcatta agcggtaaaa acaggatttt ccttatcgct ggcattctga
     5101 tgattgccac aacattacgc gtcaccttta ccggcgcagc accgttactg gatacgattc
     5161 gttccgctta ctcgctaacg acggcgcaaa ccggcttatt gaccaccctg ccattattag
     5221 cctttgcgct gatctcaccg ctggccgccc cggtggcgcg acgttttggt atggaacgta
     5281 gcctgtttgc cgcgttactt ttgatctgtg caggtatcgc aattcgctct ctcccttcgc
     5341 cttacttatt atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct
     5401 tactgccagg attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat
     5461 attccctgac aatgggagct gcggcggcac tgggatcggc gatggtagtg ccgctggctt
     5521 tgaacggttt tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct ctgctggctc
     5581 tttttttatg gctgccacag tggcgaagtc aacaacatgc aaatttgagt acctcacgcg
     5641 ccttacatac tcggggtatc tggcgttcgc cgcttgcctg gcaggtaaca ttgtttcttg
     5701 ggatcaactc actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc
     5761 aaggctatag cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag
     5821 ctcccggttt gctgatccca cttttcttac atcatgtgaa agatcagcgt ggtattgcag
     5881 cgttcgttgc cttgatgtgc gcagtgggcg cggttgggct ctgctttatg ccagcacacg
     5941 cgatcacctg gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga
     6001 cgttcattgg tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac
     6061 aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct gatgggtaaa atacacgatg
     6121 ctaacggtga ctggtccgta ccactgatgg gtgttgcaat actttcacta ctgatggcga
     6181 ttttcggact ttgcgccggg agagacaaag aaattcgcta atatccggtg ctatagtgac
     6241 gtgacaaatc atgcgtgaaa gggagaacaa atacgatgaa tattcagtgc aaacgcgttt
     6301 atgatccggc tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccacgcg
     6361 gtatcaaaaa aaccgattta gcccttgatg agtggaataa agaaatcacg ccgtcaacgg
     6421 agctgcgcaa agcctttcac ggcgaagtcg tcgattttgc aacctttcgc gagcaatatc
     6481 ttgcagaact ggcgcaacac gagcaagaag gaaagcggct ggcagacatc gccaaaaaac
     6541 agccgctgac cctgctctac tcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg
     6601 cagactggct acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc
     6661 ggatggtccc gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa
     6721 ttgttgctga ccccttgcgc tgtctgcaca ggaatgagcg tcccgccctt ctgctggcaa
     6781 tagaccgacg ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa
     6841 ggggttgaac aaccagccag gaccagcaag caaggcagaa caaaactgat aattttcatt
     6901 tattgatctc acatatttat ccaggcttag agtatcgcgg tatcgttttg ttttgcagca
     6961 ctatttttat tacattcact caaaacatat tacgccttgt ttcatctttg ttgatgatgt
     7021 tttatcatgc ctgcaaagat taaataatca gcatttaccc gccgtatcct ggagttgttt
     7081 cgtgtcagat cagattatcg cccgcgtctc gcaatccctt gccaaagaac agtcactgga
     7141 aagtctggtt cgacagcttc tggagatgct ggaaatggtc actgatatgg agtcaaccta
     7201 cctgaccaaa gtggatgtcg aagcgcgcct gcagcatata atgtttgccc gtaatagcca
     7261 gaaaatgcac atcccggaga attttaccgt ctcgtgggat tactcgttat gcaaacgcgc
     7321 cattgatgaa aactgttttt tcagcgatga agtccccgac cgttggggcg actgtattgc
     7381 ggcacgcaat cttggcatca ccacatttct gagcacgcca attcacttac cggatggatc
     7441 attctatggc acgctttgcg ccgccagcag tgagaagcgc cagtggagtg aacgcgcgga
     7501 acaggtctta cagttattcg ccggactgat tgcacaatat attcaaaaag aggcactggt
     7561 tgaacagctg cgcgaagcta atgctgcgct gattgcgcaa tcgtataccg actcgttaac
     7621 cgggctaccg aatcggcggg cgatttttga aaatctgacg acgctgtttt ctctcgcccg
     7681 gcatcttaac cataagataa tgatcgcgtt tatcgatctg gataacttca aattaatcaa
     7741 tgatcgtttt ggtcataata gtggcgatct gtttctcatt caggttggcg agcgccttaa
     7801 tacgctccag caaaatggcg aagttattgg tcgtctcggc ggtgatgagt ttttggttgt
     7861 ttcactgaac aacgagaatg cggatatttc gtcgctgcga gaacgtattc aacaacaaat
     7921 acgcggagaa tatcacttag gtgatgttga tttgtattat cctggtgcca gtcttggcat
     7981 agtagaagtc gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg ccgatatcgc
     8041 gatgtatcag gagaaaaaac ataaacagaa aacacctttt gtcacgcatt cagcgctaca
     8101 ttcctgaggc gtattcacat ccttttgatt ggtgataaca tgcgaatcgg tattattttt
     8161 ccggttgtaa tctttattac agcggtcgta tttttagcat ggttttttat tggcggctat
     8221 gctgccccgg gagcataaag atgaaaaaaa caacgattat tatgatgggt gtggcgatta
     8281 ttgtcgtact cggcactgag ctgggatggt ggtaacgtca catctaaaaa atagcaaagg
     8341 ctgcctgtgt gcagcctttg tgcaatttac gcgttaactt ttaatcttcc tgtagataaa
     8401 tagcacaaca atcgcaccaa taacggcaac cacgaagctg ccaaaattga agccatcgac
     8461 tttaccaaag ccaaacagcg tgctgatcca tccgccgact acggcaccga ctatccccag
     8521 caggatagtc ataaagaatc caccaccatc tttacctggc atgatccact tcgccagaat
     8581 accggcaata agcccaaaaa taatccatga cagaatgccc attgtttcct cacttatctg
     8641 ttttgcatta gcgggttagt cgctgataaa aagcatagca caacatcggg aggggaagat
     8701 ttgtgacgag catcacggag gaattttttg cgatggcgca gaaattgcgc catcaacgat
     8761 cagtgataat taccaaccac aaacatcatg ttcgttttcc gtgtcataag aacgcacggt
     8821 attcaccaga tcttttatca cttcagccgc cacttctggc accagcaaag tcatcggcgt
     8881 ctctgtttca taatcgacag aaacgccatt gctgttattg gtgacggtca cggtatacgt
     8941 tgctttgccc atgattcatt tcccgttatg aatgactttc cgttgttgcg cgccttccat
     9001 caggacttca ggagcaacga agaagtcaat gttgaaataa gtatcgtcag tcatggcttc
     9061 aatgttgtgc cacttttctg gagggaacac cgcaaactgc cccgcttcga taaggatcac
     9121 ctgatcaggc tctgcactgt gttcatcagc gtagccgaga tatttgaccg ccccatgcat
     9181 aacggaaagg cgtgggtaaa cccccgggcg cgttccttta tcaagatgac gttcgaatat
     9241 tccggcaggc gcagtttgtt tattccagaa aggcgttgag cgcgtatgaa tataattctg
     9301 tgggatttgc agcatccttt tccctccttc ggtgaatgcg ctggaaacgg tttattccag
     9361 ccgtttcagg gtacgcctga taatttgcat tttaaatacc atttattggt tactttttag
     9421 caccgtatca gagaagaatc agggaggatt acaggtggga agcccgcggc aacgcgggct
     9481 gaaagcatca ggattgcagc gtcgccagtc gggcggcgaa acccacgaac atcaaaccaa
     9541 tcagtgagtt gcccacttta gccagtttct ttttggtacg tatgtactgc gtgacaaaag
     9601 ccccagaaat aatcaggaag ctcaaatagc agaaactcac cagttccagc gtcgtcgcca
     9661 gaataaagaa tgaaattccc gtatgtgggg cattaacatc gataaactgt acgaaaaacg
     9721 acacatagaa caaaatggct ttcggattag tcaggctcaa aattaacgcg cgtttaaaaa
     9781 tggcaccgta ttggggctca tcggatttgg tctcgctatt tttacctttc agggtcgcgt
     9841 agagaatttt actccccaga tagagcaaat aaaacgcacc aagataacgt acgatgttga
     9901 ataatatcgg ggtggtctta attaatgtcg ccactccagc ccatgccaga aacatcaata
     9961 ccgcatcgcc aataaataca ccacaggccg caagataacc gcctttcata ccgctactga
    10021 cgctattttt gagtacaaac agggtatttg gccctggcac caacacaata aaaatggccc
    10081 caaccagata ggtccagtaa ttcagaaccc cgtattcagc gaacacatta acctctttaa
    10141 ttatctttcg atcatgcgcg attaaaggtg aatatgctaa ccaatctgta gcggcttaga
    10201 aaggagaaaa tcaggtttta actcgatatc aacccgataa ttgaatcatt aatcaggcat
    10261 gctccagtga aaaattcggg tagtgctcgg caaaatactg gcgtaaaaat tctaccgtta
    10321 tccgcacttt cgctgacgtc gacagctttg aaacataaac ggcccagaca ttcgctggct
    10381 ggtaatattc cggtagcact tgtactaaat gaccactggc gggaaacagc ctggcgacat
    10441 cgatttttac gtggtccgga aaaacaccga aggcgaatat tcctcgccag gcggtagagt
    10501 gaatgaagga acagagaatg aagtcgtcat tcaggaatcg gtatttactc gtcgtggtgt
    10561 cgagcgcatt tggcgttaag ctcaaataag ttcatttgaa ccgcctgctg ttggatttgt
    10621 agtttgccga agccggactg agcgcgccca gtccggcttc ggaagatttt actgtggata
    10681 ttcctgcaat aaattgtgta atgcttccgc catcagctca ccacgccagc cggaaatcag
    10741 ctccggcaaa ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt ggttgatttg
    10801 ccgacgcgat gccagcaatt cggcgctgat cttgtgcgtt tcgctgacgt cagtaatcag
    10861 cgacttaatc gctttaaacg ctttacgata acccggcatg tccatcaggt taagcatcgg
    10921 ctgcggtaag gcctcttccg gcaatgcctg cgctttttcc accagcgcca gcaacgtttt
    10981 accgtggaag cggatttcgc taccggataa gcccaggctg tccagttcgc ccagacttcc
    11041 cggcatataa cgcgctaccg accacaaatg ctcttcacgc acgacaaagt tcaccgccag
    11101 atcgcgctct cgcgccttgc gcaatcgcca gtcggctaac agttgcagac aggccagttg
    11161 gcgtgtgcgt aactgccagg cattggtgat atcacgccag gcgtcttccg gcgcaacgac
    11221 ttcctgacga cgcatttgca tcaggcggca ttcatccagc gccgcaggta gccagccgga
    11281 ggcctccgtt tctaccataa gctttgcggt gatcggtaac agataccaga catccgccgc
    11341 tgcgtattca cactgacgtt cggtcagcgg tctggccagc cagtcggtgc gcgattcact
    11401 cttgtccagc gtaacgccgg aatactcttc caccatggaa gcgaaacccc atgacatcgg
    11461 gcgtccgcag aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa
    11521 gacattgagg aacacttcca gatcttcact gcctgcatgg agaaattttg tgatggacgg
    11581 atcgcgcagg atcgctttca gcggtgacca gtcggtgatc ccgagtggat cgattagcgc
    11641 cagatgctca ccatcaaaaa gttgaattaa acccagctgc gggtaataag tgcgcgtacg
    11701 aacaaattca gtatccaggg ctatcgccgg aaaggcacgg acggcttcac acaaagaagc
    11761 cagcgcatcg tccgtggtaa tcatttggta attcaaattg tattctcttt agtgggcgtc
    11821 aaaaaaaacg ccggattaac cggcgtctga cgactgactt aacgctcagg ctttattgtc
    11881 cactttgccg cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga cgttagattt
    11941 cggtaactca tcacgaaact ccaccagctt cggtactttg tagcccgtga gctgacggcg
    12001 gcaaaaggtc accagtgact cttcggtaag cgatggatct tttttcacta cgaagatttt
    12061 caccgcttca ccactggagc cggaaggtac gccaacagcc gcgacttcct gtacgccagg
    12121 atgctgcatg acgacatctt caatctcgtt gggatagacg ttaaaaccgg aaaccagaat
    12181 catatctttt ttacgatcga caatgcgcag gaatccttct tcatccatca ccgcgatgtc
    12241 gccggtgtgt aaccagccat ttttgatgat ttcatcggta gcatccggac gctgccagta
    12301 acccagcatc acctgcggtc ctttgacaca aagctcacct ggttgaccag gtggtacttc
    12361 attatcatca tcatccacca gtttggcttc cgtcgacggc accggcaaac cgatgctacc
    12421 actatgataa tcaatatcat acgggttaac gctgaccagc ggcgcgcact cggtcaggcc
    12481 ataaccttcc agcagatact gtccagtcag tttaacccaa cgttctgcaa ccacttgctg
    12541 aacaggcatc ccaccgcctg cggaaagatg cagactggag aaatccagct gctggaactc
    12601 tttattgttc agcaacgcat tgaacaaggt gttaacgcca gtgatagcgg taaacggata
    12661 tttcgctaac tcttttacca gccctggaat atcgcgcggg ttagtgataa gcaggttctg
    12721 ccctcccagt tcgataaaca gcaggcagtt aatggtcagt gcaaaaatgt gatacagcgg
    12781 cagcgccgtc accaccagct ctttgcccgg atgcaacagc ggaccatagg tcgcgttaac
    12841 ctgttccagg ttcgccagca tattgcggtg agtcagcatc gcgcctttcg ccacaccagt
    12901 ggtgccgccg gtgtattgca ggaaagctaa atcttccggc accaattctg gtttgacgta
    12961 ctgcatccgg tagccgttgt gcagggcgct acgaaatgaa atggcatctg gcagatggta
    13021 tttcggcacc agacgcttga tgtatttaac aacgaaattg actaccgtgc cttttgccgt
    13081 agatagctga tcgcccatac gggtcagaat aacgtgctga acggcggttt tatcaaccac
    13141 tttttccaga gtgtgggcaa agttagacac gataacaatc gccgatgcgc cgctatcgtt
    13201 aagctgatgc tcaagctcac gcggggtata caacgggtta acgtttacga cgatcatccc
    13261 ggcacgcaaa atgccaaaca gcgccaccgg atattgcaat aagttaggca tcatcaacgc
    13321 aacgcgatcg cctttcttca gccccaaccc ttgttgcaaa taagcggcaa acgcgcgact
    13381 gcgttcttcc agtttgcgga aggtcatcac ctcccccata ttcacaaacg cgggttgatc
    13441 ggcatagcgt gcgaccgact gctcaaacat atctaccaga gattgataac ggtcagggtt
    13501 gatctccgtc ggaacgtccg cgggataacg gttaagccaa accttcttca attcttcacc
    13561 tctaaaatgc gtgttcgtcg tcatcgcaac cccaagt
//
LOCUS       DAPQZD010000129        13593 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPQZD010000129 DAPQZD010000000
VERSION     DAPQZD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.131"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(287..712)
                     /gene="arsC"
                     /locus_tag="RO454_003640"
     CDS             complement(287..712)
                     /gene="arsC"
                     /locus_tag="RO454_003640"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDY0935408.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1047..1130
                     /locus_tag="RO454_003641"
     CDS             1047..1130
                     /locus_tag="RO454_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDY0935409.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(1184..2536)
                     /gene="gorA"
                     /locus_tag="RO454_003642"
     CDS             complement(1184..2536)
                     /gene="gorA"
                     /locus_tag="RO454_003642"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDY0935410.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2608..3450)
                     /gene="rlmJ"
                     /locus_tag="RO454_003643"
     CDS             complement(2608..3450)
                     /gene="rlmJ"
                     /locus_tag="RO454_003643"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDY0935411.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3653..5695
                     /gene="prlC"
                     /locus_tag="RO454_003644"
     CDS             3653..5695
                     /gene="prlC"
                     /locus_tag="RO454_003644"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDY0935412.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5703..6455
                     /gene="rsmJ"
                     /locus_tag="RO454_003645"
     CDS             5703..6455
                     /gene="rsmJ"
                     /locus_tag="RO454_003645"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDY0935413.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPAQLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6504..7973)
                     /gene="dtpB"
                     /locus_tag="RO454_003646"
     CDS             complement(6504..7973)
                     /gene="dtpB"
                     /locus_tag="RO454_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDY0935414.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     QLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8290..8724)
                     /gene="uspA"
                     /locus_tag="RO454_003647"
     CDS             complement(8290..8724)
                     /gene="uspA"
                     /locus_tag="RO454_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDY0935415.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9115..9450
                     /gene="uspB"
                     /locus_tag="RO454_003648"
     CDS             9115..9450
                     /gene="uspB"
                     /locus_tag="RO454_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDY0935416.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9521..11020)
                     /gene="pitA"
                     /locus_tag="RO454_003649"
     CDS             complement(9521..11020)
                     /gene="pitA"
                     /locus_tag="RO454_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDY0935417.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11252..12445
                     /locus_tag="RO454_003650"
     CDS             11252..12445
                     /locus_tag="RO454_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDY0935418.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12532..13584)
                     /locus_tag="RO454_003651"
     CDS             complement(12532..13584)
                     /locus_tag="RO454_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HDY0935419.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNKLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3475 a         3445 c         3565 g         3108 t
ORIGIN      
        1 aaccactgat agatcataat ccagtacatc tttacagtgc tgcactgtat cgctgataac
       61 taagcagctc actgatacac catgctggac gcgataattg gaatctcacg cagggagtac
      121 tgtgtaaata gcaaaatcgc ctgatgcgct acgactgtca ggcctacaaa taacatacaa
      181 ttcatcgaat tatcataacg ttgtaggtcg gataagacgc gtcagcgtcg catccgacat
      241 aaataaagcg catttttcta acaacctgtg ggggaatata gccgctttat ttcaggcgat
      301 tacccgcctc atcaaccact ttctcgccat cttccttggt gaacgcgcct ttttgcgcat
      361 ctggcagaat ttccagcacg acttctgaag ggcggcacag gcgagttccc tgtggcgtca
      421 ccacaatcgg gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aactgatcgt
      481 gagtaaattt atcttctgca aggcccagtt cctcatacgg ctcgacgttt ttacgcagca
      541 acgcgcgtac ggtaatcccc atatcggcaa tgagttttac cagctcatcg cgcgtcggtg
      601 gtgtttccag ataatagata atggtcggtt ctgtaccgct gttgcggatc atctccagcg
      661 cattacgcga cgtaccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat
      721 ctcattacaa tgtgaaagag aggtgtagcg ccagcgccac cagcgttaca aaccaaagcg
      781 gacaagtcaa aaacacatat ggtttaacga atatgtaagt gtagagaaag gtgtaggtgt
      841 gaattaagcg ttgggcattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac
      901 ttcgcgtcgg tttttttact gttcagccct gagttggtgg ctctggctgg agtgagatag
      961 cccacatcta acaatgagac atgctccgtg acaggcaatg gatgagtaag cggatgcatt
     1021 ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt cttggggcgt
     1081 taattgcgca gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac
     1141 gtagctaagg gctaagagca cactactctt agccctttaa catttaacgc attgtcacga
     1201 actcttctgc tgctgtcggg tgaatggcaa ccgtgttgtc gaagtctttt ttggttgcac
     1261 ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaaa ccaatgccgt
     1321 gaataccgac aatcttctct tccggtccaa tgcataccag tttcatgcga cacggctggc
     1381 ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat
     1441 cgtcgccgta ctgctcgcgc gcctgcggct ccgttaaacc aacagtacca atcggcggat
     1501 ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa
     1561 acaggcgttc agagagacgg cgacctgctg caactgccac cggagtcagt tccactgcac
     1621 ccgtgttatc gcccaccgcg taaatacctt caacgttggt gttttgatat ttatcgacga
     1681 cgatatagcc tttttcgttg gttttaacgc cagcggcttc caggttgatg ttgtcattgg
     1741 caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga ccatcttcca
     1801 gctccagcgt caggctgccg tcggcatttt tcaccaccgc tttcgggatg gcgttggtat
     1861 gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc atcgggtcga
     1921 agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt cttcgcaccg aggccgttaa
     1981 tcacgcccgc cagttcaacg gcgatgtaac ccgcgccaac aaccgctacg cgctctggca
     2041 aagccggaag ggcgaagaag ccatcagaat caataccgta ttccacgccc ggaatatccg
     2101 ggtggctcgg acgaccaccg gtggcgatca gaatatgatc ggccgtgatg gtttcgccgt
     2161 tgacttcaat ggttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat
     2221 ttttaccgag cacgttctca taggaagtat gaatacggtc gatataggcg gtacggctgg
     2281 cgatcaatgt ttcccagttg aatttattga tagtggtatc aaagccgtaa tccgggccgt
     2341 acatatggat cgcttcacgg atttgtgccg cgtgccacat cacttttttc ggcacacagc
     2401 caacatttac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat ttctggccgt
     2461 acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat
     2521 catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg tagcgcgaga
     2581 cgtaataggt gccagcaatg gctgcaatta ctctggcacg atccagctta cggtggcgtg
     2641 cccggtgcct gctggaacca gtttgctgtg cagccacggc agcacgttat tcatctgctg
     2701 ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc cacggcgatc
     2761 gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg tcgcttccag
     2821 gtcgtggatc atgcgcttaa tctgctggcg cagcaccact ggataccaca atgcgtaagt
     2881 gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct gatagtcggt
     2941 tttcatttca tacggcggat cgataaggat taaaccacgg cgggaaaccg gcggtagttt
     3001 ggccttaagc tgctggaaac cgtcggcttt ttcaacgcgc gcacgattgt ctttctggaa
     3061 ttccgaacgt aacaacggat aatcgctcgg gtgcagttca gtcagttgca ggctgtcctg
     3121 ttcacgcagt agctggcgcg caatcaacgg cgaaccgggg taataacgca actgcccgct
     3181 acggttgaag tgttttacca cattgatgta cgcctccagc tccgcaggca aatcgtcctg
     3241 ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat gttcgctgcc
     3301 taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc
     3361 tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc
     3421 agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaatcg
     3481 gcccgtattc ggggcgcaca aaagtctatc aggacagtat accgaaaaga ggccgccgcc
     3541 gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct acaaacatta
     3601 tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa
     3661 tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt
     3721 tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg tagtagcgca
     3781 aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtgct
     3841 ggggcgtatc ttctccccgg tcagccatct gaactcggtg aaaaatagcc cggaactgcg
     3901 tgaagcgtac gaacaaaccc tgccgctgct gtcagaatac agcacctggg taggacaaca
     3961 tgaagggctg tataaagcat atcgcgacct gcgcgatggc gatcattacg ccacgctgaa
     4021 cacggcgcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct ctggcatcgg
     4081 tctgccgaaa gagaaacagc agcgttacgg cgaaattgcc actcgtcttt ctgaactggg
     4141 caatcagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac tcgttaccga
     4201 cgaagcggag ctggcgggga tgccagaaag cgcgctggct gcggcaaaag cccaggccga
     4261 agcgaaagag ctggaaggtt atttgctgac gctggatatc ccaagctacc tgccggtaat
     4321 gacctactgc gacaaccagg ccctgcgtga agagatgtat cgtgcttata gcactcgtgc
     4381 ttccgatcaa ggtccgaacg ccggtaagtg ggataacagc aaggtgatgg aagagatcct
     4441 cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct tcaaatcact
     4501 tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg atctggcaaa
     4561 acgcgcgcgc ccgcaaggcg aaaaagagct ggcacaactg cgcgccttcg ccaaagccga
     4621 atttggcgtc gatgagttgc aaccgtggga tatcgcttac tacagcgaaa aacagaaaca
     4681 gcacctctac agcatcagcg atgaacaact gcgtccgtac ttcccggaaa acaaagcggt
     4741 taacggcttg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag agcgtaaaga
     4801 tgttgatgtc tggcatccgg atgtacgttt cttcgaacta tatgacgaga acaacgaact
     4861 gcgcggtagc ttctacctcg acctgtatgc ccgtgaaaac aagcgcggcg gggcgtggat
     4921 ggatgactgc gtaggccaga tgcgtaaagc cgacggttcg ctgcaaaaac cggtcgcgta
     4981 tctgacctgc aacttcaacc gcccggtaaa tggtaaaccg gcgctgttta cccatgacga
     5041 agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga cccgcattga
     5101 aaccgctggt gtttccggga tcagtggggt gccgtgggat gcggtcgaac tgccgagcca
     5161 gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctccg gtcactatga
     5221 aaccggcgag ccgctgccga aagagttgct ggataaaatg ttggcagcga aaaattacca
     5281 ggcggcgctg tttattctgc gccagctgga gttcggcctg ttcgatttcc gcctccatgc
     5341 cgagttccgt ccggatcagg gggcgaaaat cctcgaaact ctggcagaaa tcaagaaact
     5401 ggttgccgtg gtaccgtctc cgtcatgggg ccgtttcccg catgctttca gccacatttt
     5461 tgccggtggt tatgcggcag gttactacag ctacctgtgg gccgatgtac tggcggcaga
     5521 tgccttctcg cgttttgagg aagagggcat tttcaaccgt gaaactgggc agtcgttcct
     5581 cgacaacatt ctgagccgtg gcggttcaga agagccgatg gagctgttca aacgcttccg
     5641 tggtcgtgaa ccacagctgg atgcgatgct ggagcattac ggcattaagg gctgatcatt
     5701 cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta tctgttctgg
     5761 cggcccgctg ggggctggaa cacgatgaag acaacctgat ggcgctggtg ttaacgccgg
     5821 agcatctgga actgcgcaag cgtgatgagc cgaaacttgg cggcatcttt gttgattttg
     5881 ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggagag gcggtggcga
     5941 aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc gcagggctgg
     6001 ggcgcgatgc ctttgtgctg gcttcggtcg gctgccgcgt gcggatgctg gagcgtaatc
     6061 cagtggttgc cgcgttgctc gacgatggtc tggcgcgtgg ttatgcggat gcggaaatcg
     6121 gcggctggtt acaggaacgt ttgcagttga ttcacgcctc cagtctgacg gcgctgagcg
     6181 atatcacccc gcgtccgcag gtagtttatc ttgacccaat gttcccgcat aagcagaaaa
     6241 gcgcgctggt gaaaaaagag atgcgcgtgt ttcagtcgct ggtggggccg gatcttgatg
     6301 ccgatggatt gctggagcct gcgcagttgc tggcaaccaa acgcgtggtg gtaaagcgtc
     6361 cggactatgc gccgccgctg gcgaatgtcg ccacgccaaa cgcggtagtc actaaagggc
     6421 atcgctttga tatttatgcc ggaacgccgg tgtaaaagta aagcggggta gcgataacgc
     6481 taccccgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgtttcagc
     6541 cacggcacca tcaatagcat cactactgca acgcccagcg taaccaggcc aatcttacca
     6601 aacacgttgg tatagacggg caacgtctca agtggatcgg taatgttgtc tggtactgcg
     6661 gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc
     6721 ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc caggccaagg
     6781 gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat aaaccatggc
     6841 gatgtcagcc cttgggcatc cgcaaaccac attccagccg ctgcagccgt caaaaagccc
     6901 agtgagcaca taaacatgcc gagagtaaac ttcatcggca tcgagagatc tttgcctttg
     6961 ttacccagtt gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg
     7021 ttaagcgcct ggaagctgac cgggttgatg gaaaagccga gaatttcatg atgcacgttg
     7081 ttgatggcaa agaagttcag cgacgttggc atctgggcgt agagaatgta aaacaccacc
     7141 gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg
     7201 aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag caccagattg
     7261 gcgacttcta cgttgtgcat cagccaggcg cagacgaaga tcatcaccac gctgccaagt
     7321 aatacgtaca acagtttact gaagctcatc ggccggaagt cgggttcaga accaatatct
     7381 ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc tgcaccgcac
     7441 aggttgtagg tgactgaata gccgaattta tcagcgatca caggggccag cgataacgct
     7501 atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc aagccgtgga
     7561 tctttcggcg gatagcattt cgaaagcaag ctggctgggt tagctttaaa caggccgtta
     7621 ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg
     7681 gtcatgaagt agccaatcgc cagcacaagt gctccgagaa caatggtgcg tttggttccc
     7741 agcaggtggt cgccaacata gccaccaatg gaaatgaggc catagaccag cgcagcaaaa
     7801 gcaccaaaag tgacaaaagc ctgctcttgc gagaacccaa gctgtttaac gaagaaaacc
     7861 gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc
     7921 atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt catatttatt
     7981 aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt
     8041 acatctgtca gagattctta atattgatgc ggcgaataga ttaatacact attacaacag
     8101 aaaataacca gctgatcaca ctaatgatag aaaatatgat ggtgttaaat gttgtattgg
     8161 caggggtttt aattgcgtac gcagaccgca ggccagataa ggcgtttacg ctgcatcagg
     8221 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
     8281 gagggaagat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
     8341 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
     8401 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agtacctggc ccagatcgcc
     8461 gctgccgctc agggtttcag tgattgggta gcctgcattg gttgaaagct cggtcagcgc
     8521 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat cgataagccc
     8581 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
     8641 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga ggtcgaccgc
     8701 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
     8761 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
     8821 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
     8881 cgtcaatccg ccttgcttac tcgttagata aacaaatttt cgacatgaat atattgatag
     8941 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
     9001 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
     9061 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
     9121 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
     9181 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
     9241 tatgttgatg gagggggctt ttttacctca catggccaac caaacaaaca ggtgcgtctc
     9301 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
     9361 tgtgagcggg tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc
     9421 agcctgattg cattgatgat ttggcattaa gcacttcagc aataatgacg cataaaaaaa
     9481 gcgggccagt aaactgaccc gctcgttttg gtgcgtacaa ttacaggaac tgcaaggaga
     9541 gccagtacag cccgccggaa agcagtaccg cagccggaag ggtaaacacc caggccatca
     9601 gaatactggt tacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
     9661 cagaagagga gagtacgtga gtagtggaaa ccggcatccc ggtataactt gccaggccga
     9721 tagacacagc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
     9781 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
     9841 acgccagcgc gaccgccatg atgatccaca ccggcgcata ctcgatggtg ctaagcatgt
     9901 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacgccaggc attttcacca
     9961 ctttatcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    10021 gcgacagctt gtcgtagctt tccacatcgg tagtcaacat gcctttcagg cggttgagcg
    10081 cgttaatggt attcgacgga tggcaatgga actccgcggg ttgcgttgcg ccagcttccg
    10141 gagccggtac taactgatca gcaccggtcg cttgtttcag cagtgcagga tgctgctcaa
    10201 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    10261 tcatgttcac cacgaagcct gctggtgcga cgccaatcaa taccaacata accagaccaa
    10321 tgcctttctg accatcgttc gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    10381 gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    10441 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    10501 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    10561 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    10621 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    10681 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    10741 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    10801 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaataccgcc gccataacca
    10861 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    10921 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    10981 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    11041 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    11101 tttttttgat ttgcctcctg tatggattcc actcaaaaaa taattatctt atataattca
    11161 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    11221 tcacgcccgt tttttcttag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    11281 cgctggtgcg gcaggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    11341 gctgatcgat aatggtaaaa aaccagggcg caaaatcctc atgtctggcg gtgggcgctg
    11401 caactttacc aacctttatg tcgaaccagg cgcttatctg agccagaatc cgcatttttg
    11461 taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    11521 catcgcctgg cacgagaaaa cgttagggca gctcttctgc gatgactccg cgcagcagat
    11581 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgtg gccttcagat tgcgtagcga
    11641 agtgctgagc gtggcgaagg atgatacagg cttcacgctt gaactgaacg gcatgactgt
    11701 cggttgcgaa aagctggtca ttgcgaccgg tggactgtca atgccggggc tgggcgcatc
    11761 gccgtttggt tataagattg ccgaacaatt cggcctcaac gtgctgccga cccgtgcggg
    11821 tctggtgcca ttcactctgc ataaaccgtt gcttgaagag ttacaggtgc tggcgggcgt
    11881 ggctgtgcct tccgtgatta ccgctgaaaa cggtaccgtt ttccgtgaga acttactctt
    11941 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tccagctact ggcaaccggg
    12001 tgaatttgtc agcatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    12061 gcgtaatgca catccgaatc aaagcctgaa aaacacgctg gcggttcatc tgccgaagcg
    12121 gttggttgaa cggttacaac aactcgggca aatcccggat gtctcgctaa aacagctcaa
    12181 cgtgcgtgac caacagacac tgattagcac attgactgac tggcgcgtac aacccaacgg
    12241 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc
    12301 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    12361 cgtcaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    12421 tgcgcaggat ttggtggcag aataagaagc aaaatgcgct ctcgatacca gagggattaa
    12481 cctgaataaa accagataag gcgctcaacg ccttatctga agcctttttt attatttttt
    12541 cgctgaaccc gcttctaata tagaaacgat ggaaaacagt gtaaaacaaa ctgcacctaa
    12601 gccaaccaga actcgcgaag gaataaagta agccatatcg gtttgcgcaa tttcagccag
    12661 gaacgcagca aggaacagac aaaacagaca ggtaaatacc ggaatcatgg gtattctgtt
    12721 ggctaacgaa caggtgcgtc gccataccag cgctaacaac cacactttgg agaaaatact
    12781 gtagcagatc atccccaggc aaatgagaat aatgccgggg gccaggcggg cgcttgcatc
    12841 tgaactgatt aatacgtaga taccaaaaat aatggtaagt gagccaagca aaataaccca
    12901 atagcaccac agccaatgtt ctttcactga gaacgtattc cgcgtttgat ggacaatagt
    12961 ggcgacaagg ccaataagac aagcgcaaat tgccgttaac cccattagta cgtgacctgc
    13021 aacataatgt ggtgtgatat ctgcactacg taacagtatg actgaccaaa tgaaaccaaa
    13081 gagagtaagc agaacgggaa cggcaattaa acaattaccg attgttgaag aataggcctg
    13141 caatggcgta ccgtcactct tactgcccga ggcgtttttg gggatcaaca gaaaatgtcc
    13201 ggaagatgcc gctacggttg atacacaagc ggcaatcatg ccaacgccga aaataacatg
    13261 accggcgaca aattcatctg ccatcacatt attgcttgcc aacagtgacc atccccaaat
    13321 catagttgta gcagaaccgg cataaccaat aacaggaaat aacttgttgt agaatttatt
    13381 catgccgtga gttaactgag atataataat aaatgcggta gtgaacaatg ccagacatat
    13441 cgcagaaaga gaaatcagaa catgccctgc aacgaaatat tcactttgcc cagaaccggt
    13501 taatacaaac tcacctaatg cgatgcatat tatacccatc agtaaaggaa ttaatttaaa
    13561 caaccatccg atatatatat tcattttaaa ccc
//
LOCUS       DAPQZD010000130        13474 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPQZD010000130 DAPQZD010000000
VERSION     DAPQZD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.132"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..2212
                     /gene="crfC"
                     /locus_tag="RO454_003652"
     CDS             <1..2212
                     /gene="crfC"
                     /locus_tag="RO454_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDY0935420.1"
                     /translation="YELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLALPWSQPN
                     IAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTL
                     IRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIEN
                     GIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAG
                     LESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREA
                     ILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYL
                     ANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSL
                     FAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIH
                     QVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQDNSESLS
                     EMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRP
                     GFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPDWGRGNE
                     KFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFASCLTELQ
                     TRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            2209..3087
                     /locus_tag="RO454_003653"
     CDS             2209..3087
                     /locus_tag="RO454_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDY0935421.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3351..4853
                     /gene="proP"
                     /locus_tag="RO454_003654"
     CDS             3351..4853
                     /gene="proP"
                     /locus_tag="RO454_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDY0935422.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4965..5054
                     /gene="pmrR"
                     /locus_tag="RO454_003655"
     CDS             4965..5054
                     /gene="pmrR"
                     /locus_tag="RO454_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDY0935423.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5030..6130)
                     /gene="pmrB"
                     /locus_tag="RO454_003656"
     CDS             complement(5030..6130)
                     /gene="pmrB"
                     /locus_tag="RO454_003656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDY0935424.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(6131..6799)
                     /gene="basR"
                     /locus_tag="RO454_003657"
     CDS             complement(6131..6799)
                     /gene="basR"
                     /locus_tag="RO454_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDY0935425.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6796..8439)
                     /gene="eptA"
                     /locus_tag="RO454_003658"
     CDS             complement(6796..8439)
                     /gene="eptA"
                     /locus_tag="RO454_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDY0935426.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8543..9880)
                     /gene="adiC"
                     /locus_tag="RO454_003659"
     CDS             complement(8543..9880)
                     /gene="adiC"
                     /locus_tag="RO454_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDY0935427.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10017..10778)
                     /gene="adiY"
                     /locus_tag="RO454_003660"
     CDS             complement(10017..10778)
                     /gene="adiY"
                     /locus_tag="RO454_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDY0935428.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11114..13381)
                     /gene="adiA"
                     /locus_tag="RO454_003661"
     CDS             complement(11114..13381)
                     /gene="adiA"
                     /locus_tag="RO454_003661"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDY0935429.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3252 a         3276 c         3553 g         3393 t
ORIGIN      
        1 gtatgagtta agtcaggagg ctgaacgcct gttacagctt tcccgacaac agttgcagtt
       61 actggaaaaa atacctctct ctgtacccgg agacgatgcg ccacaactgg ctttaccctg
      121 gagtcagcct aatatcgccg aacgtcacgc gatgctgaat aatgagttgc gtaaaatttc
      181 ccggctggaa atggtgctgg cgattgtcgg taccatgaaa gcagggaaat caaccaccat
      241 taatgccatt gttggtacgg aggttctgcc taatcgcaat cgcccaatga ctgcgctgcc
      301 aacgcttatt cgccatacgc ccgggcaaaa agaaccggta ctgcattttt cacatgtcgc
      361 gccaatcgac catttaattc aacaattaca acagcgcctg cgtgattgcg atattaagca
      421 tctgaccgat gtgctggaaa tagataaaga tatgcgtgcg cttatgcagc ggatcgaaaa
      481 tggcatcgct ttcgaaaaat attatctggg tgctcaacct atttttcatt gtctgaaaag
      541 tttgaatgat ttggtgcgac tggcgaaggc gctggacgtc gattttcctt tttctgctta
      601 cgccgccatt gagcatattc ccgtgattga agtggagttt gtccatctgg cggggctgga
      661 gagttatccc ggtcagttga cgttactgga tacccccggg ccaaatgaag ccgggcaacc
      721 gcatctgcaa aaaatgctta accagcagct ggcacgcgcc tcggcggtac tggcggtgct
      781 ggattatacg caactgaaat cgatctccga tgaagaggtc cgtgaggcaa ttttggcggt
      841 ggggcaatcg gtgccgttgt atgtgctggt caataagttc gatcaacagg atcgtaacag
      901 tgacgacgcc gaccaggtgc gggcactgat ttccggaacg ctgatgaaag gctgtattac
      961 gccacagcag atatttccgg tgtcgtcgat gtggggctac ctggcgaatc gggcgcgcca
     1021 tgagttagcc aacaacggta agttacccgc gccagagcaa caacgctggg tggaagattt
     1081 tgcccatgcc gcgctcggca ggcgctggcg tcatgccgat ctggcggacc tcgaacatat
     1141 tcgccatgct gccgatcagt tgtgggaaga ttcgctgttc gcccagccaa ttcaggcgtt
     1201 gcttcatgcc gcttacgcta acgcctcgtt gtatgctctg cgatctgccg cgcataaact
     1261 gttgaattac gcgcagcagg cgcgggaata cctggatttt cgtgcgcacg ggttaaacgt
     1321 cgcttgtgaa caattgcggc aaaatatcca tcaggtcgaa gaaagtttgc agctattgca
     1381 actcaatcag gcgcaggtga gcggcgagat taaacatgaa atcgagttgg ccctgacctc
     1441 cgccaaccac tttctgcgtc aacagcaaga tgcggtgaat gcgcagttag ccgcgttgtt
     1501 tcaggataat tcggagtcgt taagtgagat gcgtacccgc tgtgagacac tgttacagac
     1561 ggcgcagaac accatcagtc gcgactttac gctgcgcttt gccgagcttg agtccaccct
     1621 ttgccgggtg ttaaccgatg ttattcgccc cattgagcaa caagtcaaaa tggaattgag
     1681 tgagtctggg tttcgtcctg ggtttcattt tcctgttttt cacggcgtag ttccccactt
     1741 caacacccgc cagctgttca gtgaagtcat ttcgcgccag gaagcaacgg acgagcagag
     1801 cacgcgttta ggcgttgtgc gtgagatttt ttcgcgctgg ttgaatcagc ccgactgggg
     1861 acggggaaat gagaaattcc cgacagagac ggttgattac agtgtgttgc aacgagcatt
     1921 aagcgcagaa gtcgatcttt attgccaaca aatggctaaa gttatggcag agcaggtcga
     1981 tgaatctgtt acggcaggca tgaatacttt tttcgctgag ttcgcttcat gtttgacgga
     2041 attacagacg cgtttacgcg aaagtatggc tctgcgtcaa caaaatgaat cggtggtcag
     2101 gctgatgcaa cagcaattgc agcagactgt gatgactcac ggctggattt acaccgacgc
     2161 tcagctgtta cgcgatgata ttcaaacact tttcacggca gaacgatatt gaccaagacg
     2221 ttactggacg gccccggtcg cgtgctggag tcggttcatc cgcgcttttt agtggatctg
     2281 gcgcagggcg atgatgcccg ccttccacaa gcccatcagc agcagtttcg tgaacgactg
     2341 atgcaggaac ttcttgcgcg cgtgcagctt cagacatgga cgaacggcgg catgttaaat
     2401 gcgccgctta gcctgcgtct gacattggtg gaaaaactgg cgtcgatgct ggatcccggt
     2461 catctggcgc tgacgcagat cgcgcagcat ctggcgctgc tgcaaaaaat ggatcaccgc
     2521 cagcactctg ctttcccgga gctcccccag caaattgtcg ccttgtatga gtggttttca
     2581 gcccgttgtc gctggaagga aaaggcgtta acgcaacgag gcctattggt gcaggcgggt
     2641 gaacagagcg agcaaatttt tacccgctgg cgtgctgggg cgtataacgc ctggtcgttg
     2701 cctgggcgct gttttatcgt tctcgaagag ttgcgctggg gtgcatttgg cgatgcctgc
     2761 cgtctgggaa gcccgcaagc ggtggcgttg ttgctaggtg atttgcgcgt gaaagcgaca
     2821 caacatttgg cagagagtat caatgcggcc ccgaccacgc gtcactatta ccatcagtgg
     2881 tttgcctctt cgaccgtttc gacgggcggg gatcatgctg attttttaag ttggctggga
     2941 aagtggtcta cggcagataa acaacccgtt tgctggtcag tgacccaacg ctggcgaact
     3001 gtcgcactgg ggatgccacg actctgttca gcgcagcgtc tggcgggggc aatggtcgag
     3061 gaaatcttct ctgtaaattt ggtgtaaata atcagttaca ccaatgagtc ctaaacgaaa
     3121 tgcatgtgtg aagttgatca caaatttaaa cactggtagg gtaaaaaggt cattaactgc
     3181 ccaattcagg cgtcaactgg tttgattgta cattccttaa ccggagggtg taagcaaacc
     3241 cgctacgctt gttacagaga ttgcatcctg caattcccgc tccccttttg cggccgtcgc
     3301 gctgattttt ctggcgtttg cggaaatggg ccaactctgc gaggaaagct atgctgaaaa
     3361 ggaaaaaagt aaaaccgatt acccttcgtg atgtcaccat tattgatgat ggtaaactgc
     3421 gtaaagccat taccgcagca tcactgggta atgcaatgga atggttcgat tttggtgttt
     3481 atggttttgt tgcttacgca ttaggtaaag tttttttccc gggggctgac cccagcgtgc
     3541 agatggttgc tgcactcgcc actttctccg ttccctttct gattcgaccg cttggcgggc
     3601 tcttctttgg tatgttgggc gataaatatg gtcgccagaa gatcctcgct atcactattg
     3661 tgattatgtc gatcagtacg ttctgtattg gcttaatacc gtcctacgac acgattggta
     3721 tttgggcacc gattctgctg ttgatctgta agatggcaca aggtttctcg gtcggcggtg
     3781 aatataccgg ggcgtcaata tttgttgcgg aatactcccc tgaccgtaaa cgtggcttta
     3841 tgggcagctg gctggacttt ggttctattg ccgggtttgt gctgggagct ggcgtagtgg
     3901 tgttaatttc gaccattgtc ggcgaagaga acttcctcga ttggggctgg cgtattccgt
     3961 tctttatcgc tctgccgtta gggattatcg ggctttacct gcgccatgcg ctggaagaga
     4021 ctccggcgtt ccagcagcat gtcgataaac tggaacaggg cgaccgcgaa ggtttgcagg
     4081 atggcccgaa agtctcgttt aaagagattg ccaccaaaca ctggcgcagc ctgttgacat
     4141 gtattggtct ggtaattgcc accaacgtga cttactacat gttgctgacc tatatgccga
     4201 gttatttgtc gcataacctg cattactccg aagaccacgg ggtgctgatt attatcgcca
     4261 ttatgatcgg tatgctgttt gtccagccgg tgatgggctt gctgagtgac cgttttggcc
     4321 gtcgtccgtt tgtgctactt ggtagtgttg cactgtttgt gttggcgatc ccggcgttta
     4381 ttctgattaa cagtaacgtc atcggcctga tttttgccgg gttactgatg ctggcggtga
     4441 tccttaactg ctttacgggc gttatggctt ctaccttgcc agcgatgttc ccgacgcata
     4501 tccgatacag cgcgctggcg gcggcattta atatttcggt gctggttgcc ggtctgacgc
     4561 caacactggc ggcctggctg gtcgaaagct cgcagaatct gatgatgcct gcctattacc
     4621 tgatggtagt ggcggtggtt ggtttaatca ccggcgtaac catgaaagag acggcaaatc
     4681 gtccgttgaa aggtgcgaca ccggcggcgt cagatataca ggaagcgaag gaaattctcg
     4741 tcgagcatta cgataatatc gagcagaaaa tcgatgatat tgaccacgag attgccgatt
     4801 tgcaggcgaa acgtacccgc ctggtgcagc aacatccgcg aattgatgaa taagctgaaa
     4861 cggatggcct gatgtgatgc tgtcttatca ggtcaattga actcttaagg ttcacttaat
     4921 ctctgacgcg catactctcc tccaggttaa cggaggagag tgcaatgaaa aaccgtgttt
     4981 atgaaagttt aactaccgtg ttcagcgtgc tggtggtcag cagctttctt tatatctggt
     5041 ttgccacgta ctgatctttt ttcagccgta tccaggcccg cgtgccggaa gtctcttgtc
     5101 ggttttgcag gaaaaactgc ccgtgatgca actgtgtaat gcggctgaca atacttaacc
     5161 ccagaccaat tccgccataa cggctgtcca ttcgtacaaa tgctttactc aactccccgc
     5221 atttactctc atcaataccc ggtccttcat cttcaacggc catgaccgct ccgccatctt
     5281 cttgcagctt aatcataatg ttgctgcctt gcgggctata acgatgggcg ttttctacca
     5341 ggtttcgcaa taacatccgc agcagggttg catcaccctg aacggtgatg tcggcggcgc
     5401 tctctggcaa tagcagggtt tgctggcgct ggtcgagcat ggtactgagt tcgtcatacg
     5461 aggggagaat gacatcttcc agcagtttta catgttgata attaccggaa gaaaatgact
     5521 gtccggcacg cgccagttgc agcaactggg agacgctctc catcatctga tcaagtcgtg
     5581 ccactaacgg tgctacatca atgtgatgcg ttttcgccag cagttccaga tgcaaacgca
     5641 cccccgccag tggcgttcgc agttcgtgcg cgacgtcagc ggtaaacaac ctttcattat
     5701 cgagcgtgct ggtcaggcga ctgaccagat cgtttaacgc cgaaaccacc gcatcgattt
     5761 cgagggtggc gctatgaatg gcaatgggcg ttaagttgtc ggcggtgcgc gcttccagct
     5821 ctttttgcag ctccgccagc ggtcgggtga tgcggcgtac cgcctgatag cagataaata
     5881 gcgtcaggct aaccataaag acgccgggga caatcaggct ggcgaccgcc tcgcggatct
     5941 cacgcatgat gtggcgatcg ttgttgcgat tgtcgcgtag cgcctgctca aacagctgaa
     6001 tctgctcggt actttcatgc catagccaga agacgctgat cagctcaaac accaacaaaa
     6061 tggccccgat ggtcaatatc agccgttggc gcagcgatat tggtcggcgc agaaaacgca
     6121 tcagattcaa ttagttttcc tcatttgcga ccagcatata gccaaatccg cgcacggtgc
     6181 ggatacgggc tttgcccact ttgtcgcgca ggttatggat atgcacttcc agggtgttgg
     6241 tcgagggctc gttgtcccag ttgtagatat cgttgtagag aatttcccga tgcaccgggc
     6301 tgcctgcttt aagcatcagg cgcgacagca gagcatattc tttgggcgtc agaatcaact
     6361 cttcaccgcc catccagacc tgacggcgac ccatattcag cgtcaggttg ccaacaatca
     6421 gctcactttc gccctgatta ttatggcgtc gtagcagggc gcggatacgg gcgtgcaact
     6481 cttccagcgc aaaaggcttc accagataat cgtcggcccc gacatccagc ccggcgattt
     6541 tgtcggtaag ggtatcgcgg gcggtgagaa ttaacaccgg cagagtgtat tttttctgcc
     6601 ggatacgggc aagaaaatgc agcccatctt catcgggtaa gcctaaatcc agtaccacca
     6661 ggctgtaatg accggcttca aggctttgtt ccgccatccg cgcggtgctc acgccatcgc
     6721 acgcgtagcc ttcggtttgc gccgccagaa tcaatccctg caataacagc gtatcgtctt
     6781 caacaatcag aattttcatt cactcactct cctgcaagtt tgcagaatat catccgcagc
     6841 ctggtaatac ttcgtctcaa cgccagtcag gcccaataag gttgagaata aattgtcttg
     6901 tgaatagtgt tgcgtttgcg cctgtttttg caggcagttc tggtcaacct gataccgttt
     6961 ttgataatcc tccgatagcc acagcagcat cggcacctgt ttttggctat ccggggcgat
     7021 ggcataaggc agaccgtgca gatagatgcc attttcacct aacgattcac cgtggtcaga
     7081 aagataaacc aggctggtgg taaatttatc ctgatgttct ttcagcagat taatcgcttt
     7141 atcaacaata tagtcgacgt aaaccagcgt gttgtcgtaa gtgttcacca gttgctcttg
     7201 ggtacaggtc tggatctcgt tagtgtcgca ggttggggta aatttcctga actgcggcgg
     7261 atagcggttg taataggtcg ggccgtggct gccgatggtg tgtaagacaa tcacgccatc
     7321 accctgcagg ttattgatgt aatcttccag cccgtggaac agcacttcat catagcattc
     7381 gccgttgatg cactgaccag gcaggttcag cgcggtgacg ttctggtgag gtacgcgatc
     7441 gcaaacgcct ttacagccgc catcgttgtc attccacagc acgttgatgc ccgctcgctg
     7501 aatgatatcc agcacgcctt cctggtgctg tgccagctct tctttgtagt gctcacgcgg
     7561 catatccgag aacatgcatg gtactgaaac tgccgttgcc gtgccgcaag aggcggtatt
     7621 agggaaatag accacgttat cttttgccag acgaggattg gtttcgcgtg gataaccatt
     7681 cagcgagaaa ttctctgcgc gcgacgtttc tccgacgatc aaaatggtca ggtttttacg
     7741 tttcccattt tgcattaatg ggttgcggtg agcatcttca ccaatgcgta tcagtggcag
     7801 gttggctgac cgctggtgag agtaccacga ccagctagca acaatgctgt tggacgggct
     7861 taaggatttc accagttctt tgttgttgcg aaacagcgag gcatagtctt tgtagaacag
     7921 tgcggcaacc agcagaatca gcagtacaga aatcaggatg ttggccccgc ggaaaagaac
     7981 gctgcgcagg cgtgaggtgg tgggtttgat tttgatccag caggcaataa tcgcggctaa
     8041 tacgccactt aaccccagcg ttaataacat tcgtggcgtc atcagggcat aactttccgc
     8101 cggcgtagta tcaatgatat tggtgatcat tgaacggtcg atgacgatgc cataggtcat
     8161 gatgaaatat tgcgcagccg caccgaccag aataaacagg caggcgagtg gacgattaag
     8221 ccataagaaa gagcctagtg tcaggacaat attaatcacg ctgaatgcga cgaccggcat
     8281 cgacaagaaa accagcacgt tatgcagtga atccagcggt aacacctgta gcacctgttt
     8341 aaaaaaggcg atattcaggc agactgagat ataaaaagta gcaagtaata accaggagaa
     8401 taaattcaaa gagggtcttt ttagtaggcg cttcaacatc acggtgtttc catcgaacaa
     8461 agtgcgcata tgctcgcaaa gcaaaattaa gccaacctta agttcttaag gttggctttt
     8521 atgtttgctg gattaagggg aattaatctt tgcttattgg tgcatctaag ggatacgggt
     8581 ttttatgcag ccggttgtaa ttcagcgcat acatggcggt gattaccatc agagtgacaa
     8641 atgaccacat aacctctttc gcgccggacc ctactacggc ccagatgcag tagaggaagg
     8701 caatggtagt aactgccagg tatgccgggc gcgctttacc aaagtgaccg tggccgagca
     8761 gcagtaacgc cgcacaggtg taaagatatg gcaccagtgt aaagatgacc gacacggaag
     8821 aaaccagacc gaattctttg gtcgcgtttg gcgaaatgct actgagctgg aagatggtca
     8881 tcaaaatacc gacgataatc agccccgcca ctggcgtgcc cgctttattt acacgggcaa
     8941 aaatcggtgg gaacagtccg tcatcggcag cggctttcgc cgtttgacct gccagcaacg
     9001 tccagccgcc cagtgaacct aagcaacccg cagctgcgca gaaggaaaca atggccccgg
     9061 cggtgtcacc caacgccatc cgtgcggcat caccgaatgg cgaagcagaa acgcgcagtg
     9121 cggcattagg gatcatcccc ataatcgcgg tggtagaaag tacatagcaa acggcagcaa
     9181 tcaatacccc gccaatggtg gcgataggga cgttgcgttt cgggtttttc accacacctg
     9241 cggcaacgga ggcactttcc acaccgatga acgaccacag cgtaacgtta agggtacttt
     9301 gaatcgcacc gaaggtgccc aggccgctga cgttccatgc cgccatatag gtttcaccac
     9361 ggaaccagaa ccagccaaat acggcaatcc cgacgatggg gatcagcgcc agcacggtag
     9421 caacggcctg tacgcgggtg atcattttcg gaccgacaat gttcagcagg acgaagatcc
     9481 acagcaccac gacgcaggtg atggttaata ccaatggatc tttcagaatc gggaagaagt
     9541 aacttaaata tcctacgcca atgaccacca tggcgatatt gccgatccag caggccagcc
     9601 agtagaggac gttggtttga taaccgagaa acggcccaaa gcagcggcgg gcgtaagcgt
     9661 aagaaccacc aggacttggg tcgaggaacg acattttggc gtataccatc gagagcccca
     9721 gcgcaccgat aatcgtcacc aaccatccat aaatggcaat cccgccagtg gaggccaggt
     9781 ttgcaggtaa cagaaaaaca cctgacccca taatattccc cgacaccatc agggtgacgg
     9841 ggattaagcc cactttgtga gcatcagcat ccgaagacat aattaaactc ctgcgaaggc
     9901 gagcttcgtg acaataaatt acgtcatatc atacgcctgc attcgatagt tgaattatta
     9961 tcgaccgggt tatcgctgat gccgttatta atatagctct tcgttaaggt taaaaatcag
    10021 gcgacagtgc gttctctgtg ctgactaaca taatgcagag gcgtcatacc gtagaagtct
    10081 ttaaagacag aaataaagta cgacgtactg ttgtagccgc aggactgtga tacctgagag
    10141 atatttttac cgtccatcat taattcattt acggcatagc gcatgcggca ggtggtgatt
    10201 atttggctat aactggtgtt ttcgcttttc agctttttct ttaacagact tgggctaaga
    10261 cataaacaac tggctaccat actaagattc cagtctttgt ggatatcgct ttcaataatt
    10321 tgataaacgc tgtcacttac acaattacgt aacatataca tcattagtga aaggaatttt
    10381 ttactgtcga gaaaacggga tagcacagtg aataataatg cacgtgttcg ttccttttct
    10441 gactcagttt cagcgggcat catgctatgt tgcgcggcaa cgcgaaagac gtctggcgtc
    10501 aggcatggaa gggtgaggat gggcgtagcg ctacgttgcc atactggtat ttgcgagaga
    10561 tctttattca gaaacctgag gtaatctttg atgatgtcgt gactaatatg agcaaccaaa
    10621 gtgttattaa gagaggagac gtcgataata ttattttcac aatttaataa cgccatatgg
    10681 ttagctttaa ggctaatagg ttctttccca ttcaccctta tccagacatc tttttcagtc
    10741 aataaaacaa tacaaggttg gtcgctgcaa atcctcatac atgtactcct gagtgcgaat
    10801 aaaagaccag ttctcgctaa agcgaagcga taccgggaaa tggttatata attgtatgat
    10861 gaatataaac atgcataaaa ataacgagtt ttactaatat tacaaatttt tatagtaatt
    10921 tctcatgtac atattttttt acaggctaag aaaaggataa ataacaggca aaaaaaagcg
    10981 cggggtggga ccgcgcaaaa agcatgacat tttttgtttg cagcaatttt tatgaaaacg
    11041 ttgccatact taaaaggcct caagccaggc ggcgtaatgt tatttaacaa ttacgccttc
    11101 agcggaatag tggttacgct ttcacacaca taacgtggta aataccgtca ataatttctg
    11161 tcccttctgt ttcgtgttca aatccaggga aatgatgatc ccaggattgc agcgagcgta
    11221 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
    11281 ttcctggcgg atacgggata actgagtttg ccgcgatacg tcctggcaga ttttcaatgg
    11341 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
    11401 ccaccggtag gccggaatag gcttcattca accgtgcgcc agggttgttt tctttcagcc
    11461 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
    11521 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
    11581 aggagcaaag ggtgttaacc agagttcccc atttcccacg ggtcacgccc atagagaaca
    11641 ggaacataat ctggaagtca gtggtacggg taggcacaat gccgtggcga ccaagccagg
    11701 ctgtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
    11761 gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga
    11821 agccgtgcca gctttcgccc ggatgcatga cccagcagtc ctgaacggtg gtcagcagtt
    11881 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
    11941 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca
    12001 tcgcctgacg gaaatcaacc gcttcgtcga tcacttcctg ggtcagtgac aggccgctgt
    12061 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
    12121 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
    12181 cacgtacatg aatataagag gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
    12241 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
    12301 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
    12361 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtac
    12421 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttagtc agcgggcttt
    12481 cattgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
    12541 tgccgtagcg gttgcggctt ggcaccatgt agaccggttt cgcacctgtc agcatcaagc
    12601 cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc
    12661 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
    12721 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
    12781 gcaaagaacc gagggaagtg cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
    12841 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
    12901 ctggcgctgc ccaggaatat tcatggatgt cgctatattt catcagcgcg ctgaacagtg
    12961 gcggcaatag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
    13021 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca
    13081 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
    13141 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
    13201 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
    13261 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
    13321 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
    13381 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
    13441 gggctgtaaa gcgcgtgaaa aacaaacatt tttg
//
LOCUS       DAPQZD010000131        13311 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPQZD010000131 DAPQZD010000000
VERSION     DAPQZD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.133"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            217..1314
                     /gene="mltA"
                     /locus_tag="RO454_003662"
     CDS             217..1314
                     /gene="mltA"
                     /locus_tag="RO454_003662"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDY0935430.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1391..2197
                     /gene="tcdA"
                     /locus_tag="RO454_003663"
     CDS             1391..2197
                     /gene="tcdA"
                     /locus_tag="RO454_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDY0935431.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2248..2691)
                     /gene="csdE"
                     /locus_tag="RO454_003664"
     CDS             complement(2248..2691)
                     /gene="csdE"
                     /locus_tag="RO454_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDY0935432.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2691..3896)
                     /gene="csdA"
                     /locus_tag="RO454_003665"
     CDS             complement(2691..3896)
                     /gene="csdA"
                     /locus_tag="RO454_003665"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDY0935433.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4088..4315
                     /locus_tag="RO454_003666"
     CDS             4088..4315
                     /locus_tag="RO454_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDY0935434.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4666..5583
                     /gene="gcvA"
                     /locus_tag="RO454_003667"
     CDS             4666..5583
                     /gene="gcvA"
                     /locus_tag="RO454_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDY0935435.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGENPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5602..5997
                     /locus_tag="RO454_003668"
     CDS             5602..5997
                     /locus_tag="RO454_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDY0935436.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5990..7090
                     /gene="rlmM"
                     /locus_tag="RO454_003669"
     CDS             5990..7090
                     /gene="rlmM"
                     /locus_tag="RO454_003669"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDY0935437.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7134..7865)
                     /gene="fucR"
                     /locus_tag="RO454_003670"
     CDS             complement(7134..7865)
                     /gene="fucR"
                     /locus_tag="RO454_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDY0935438.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7923..8345)
                     /gene="fucU"
                     /locus_tag="RO454_003671"
     CDS             complement(7923..8345)
                     /gene="fucU"
                     /locus_tag="RO454_003671"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDY0935439.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8347..9765)
                     /gene="fucK"
                     /locus_tag="RO454_003672"
     CDS             complement(8347..9765)
                     /gene="fucK"
                     /locus_tag="RO454_003672"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDY0935440.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATSIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9874..11649)
                     /gene="fucI"
                     /locus_tag="RO454_003673"
     CDS             complement(9874..11649)
                     /gene="fucI"
                     /locus_tag="RO454_003673"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDY0935441.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11682..12998)
                     /gene="fucP"
                     /locus_tag="RO454_003674"
     CDS             complement(11682..12998)
                     /gene="fucP"
                     /locus_tag="RO454_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDY0935442.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3216 a         3301 c         3500 g         3294 t
ORIGIN      
        1 accccgcgtc cgtggatgcg cactatactc tgctcgcgtt ttcatgcaac ctttttttta
       61 ccccgatcac ggatttggca tgattttgaa caaaaacaaa tcactagagc ctattttttg
      121 tgcaaaattt gcgtcaggga gaggctgctc atttcattaa acgcggtggt ttgttatctt
      181 cgttgcgcct tattttttaa cctgaagaag agaacaatga aaggacgttg ggtaaagtac
      241 cttcttatgg gcacggttgt ggcaatgctt gccgcctgct cttccaaacc aaccgatcgc
      301 ggacagcaat ataaagacgg gaaatttacc cagcctttct ctctggtgaa ccagccagat
      361 gccgttggcg cgccgattaa tgccggtgat tttgccgagc aaattaacca tatccgtaat
      421 tcgtcaccgc gtctgtatgg caaccagagt aatgtttata acgcggtgca agagtggctg
      481 cgcgcaggcg gtgatacccg caatatgcgc cagttcggca ttgatgcctg gcagatggaa
      541 ggtgtcgaca actatggtaa cgtgcagttt acaggctatt acacgccggt aattcaggcg
      601 cgccataccc gtcagggcga gttccagtat ccgatttacc gtatgccgcc aaaacgtggt
      661 cgtctgccgt ctcgtgcgga gatctacgca ggagcgctga gtgataaata tattctcgct
      721 tacagtaact ccctgatgga taacttcatt atggatgtgc agggtagcgg gtatatcgac
      781 tttggtgatg gcagtccgct taactttttc agctatgcag ggaaaaacgg tcatgcctat
      841 cgcagcattg gtaaggtgtt gatcgaccgt ggcgaagtga aaaaagaaga tatgtcgatg
      901 caggcgattc gtcactgggg cgaaacacac agtgaagctg aggttcgcga gctgctggaa
      961 cagaacccgt ctttcgtctt ctttaaaccg caatcttttg caccggtgaa aggggcaagt
     1021 gcggtgccgc tggttggccg tgcttctgtt gcctccgatc gttcaattat tccgccaggt
     1081 actaccttgc tggcagaagt gccgctgctg gataataacg gcaaatttaa cggtcagtac
     1141 gaattgcgtc tgatggtggc gctggatgtc ggtggcgcaa tcaaaggcca acacttcgat
     1201 atctatcaag ggatcgggcc ggaagccgga caccgcgcag gttggtacaa ccactatgga
     1261 cgtgtctggg tgctgaaaac cgccccgggc gcaggtaacg tctttagcgg ctgatgtgat
     1321 attctgtatg ccgtttttac ggatatgaca gggtgagggg taacctcacc ctttttaatt
     1381 ctgaggtgct atgtctgtgg taattagtga tgcatggcgt cagcgttttg gtggcacagc
     1441 gcgtctgtat ggtgaaaaag cattgcaact gtttgctgac gcgcatattt gtgtggttgg
     1501 tatcggtggt gtcggttcct gggcggcgga agcgctagcg cgcacgggga ttggtgcaat
     1561 cacgcttatc gatatggatg atgtgtgcgt caccaatacc aatcggcaaa ttcacgctct
     1621 gcgcgataac gtcgggctgg caaaagcgga agtcatggcg gagcgtattc gccagattaa
     1681 cccggagtgc cgtgtaacgg tagtggatga cttcgtgacg ccggataacg tagcgcaata
     1741 tatgaatgcc ggttattcgt acgtgattga tgctattgat agtgtacggc ccaaagcggc
     1801 gctgattgct tattgtcggc gcaataaaat cccgctggtc acgaccggtg gcgctggtgg
     1861 gcagattgac ccgacgcaga ttcaggttac cgatctggcg aaaacgattc aggacccgtt
     1921 ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg
     1981 taagctcggc gtggattgcg tgttctctac cgaagcgctg gtgtacccgc agtcagacgg
     2041 caccgtctgt gcgatgaaag ccacggcgga agggccaaag cggatggatt gtgcctcagg
     2101 atttggcgcg gcgacgatgg tgaccgccac ttttggcttt gttgcggttt ctcatgcgct
     2161 gaagaagatg atggcgaaag cggcgcgtca ggggtaaaca atcaggtcgg atgcgacgcg
     2221 aacgtcttat ccgacctggt tctgtgctta aacctgcttc gccgcagcga taatcgcctc
     2281 gcttaacgca ttcaacccct ggctgcgtga ggcgctaagt tgcgcacgta atcccagctc
     2341 atcaaacaat gccagcggcg actgtgcctg caattcggca gcggttttcc cctcaacggc
     2401 agtcaacaac accgccaaca ggccgcgcac aatgcgccct tcgctgtcgc caaagaaatg
     2461 catcttgccg ttttcagcca cggtatatcc cagccagacg cggttttcgc atccggcaat
     2521 ctctttagcc tgcgctttta actcctctgg caaagccgga agctgtttcc ccagcatgat
     2581 caactgccga tatttatctt cccattgcgt caacggtgcg aaggtattgc gtaacgtttc
     2641 tgcggttacg gttgtgccga acggatgtcc ggcgaattgc gggtttgtca ttaatccacc
     2701 aataattcca gcgcgcggtc aacggcattc accagcgcat ccacatcact ctttgtatta
     2761 tatggcgcaa atgaggcgcg cagtgtgccg gttacgccta attctgccag tagcggctga
     2821 gcgcaatgct gcccggcgcg cagggcaata ccgtactccg ccagcagtgt caccatatcg
     2881 ctgtgatgaa ctccagcaaa atcaaaggcc agcaggctgg aatcctggca gcggaatgaa
     2941 cgaaagcctg gacgtttcgc cagcgcttct tctgcaagcg ttgctaagct acggctccag
     3001 ctttcggctt ggttgatatc gtaatctgtc agccattcca gcgccgcgct taatcctatg
     3061 acgccagcga catttggcgt tcctgcttcc agtttccacg gcgcagattg agtcgtgaaa
     3121 ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca gccagggcga catcgcttcc
     3181 agcagttctg gtttgccata cagcacgccg atgcctgtcg gaccatacag tttgtgacct
     3241 gaaaaagcat agaaatcaat atccagttgc tgaacatccg cggggaaatg caccgccccc
     3301 tgagcaccat caaccatcac caccatcccg gctgaatgag caaaagtaat cgctctcgcc
     3361 agatccgggc aaccgccagt gacgttcgac atctggccca acgccagaat ccgactacgg
     3421 ggagtaatca gttctggcaa caaatcgaca tccggcagtc gctgcgcatt aagcggcaat
     3481 ttcaccactt tggcccccgt ttgttgggcg accatcagcc aggggacgag gttggcgtgg
     3541 tgttctgcca cactgacaat aatttcatcg cccggttgca gacgcggacg ggcatagcat
     3601 tgcgccacca tgttgatgga ttcagtggtg ccgcgcgtcc agacgatatt tttgtcatcc
     3661 gatgcattca gtaattgtgc cactttttcc cgcgcagctt cgtaacgcgc ggtcaggcgt
     3721 tgggcttcgg caaactggct gcgatggacg tttccggcgc tcaggctata aaactgtcgg
     3781 gaggcttcaa ccacggcttc aggtttaagc gcggtcgcgg cgctgtcgag atagacgccc
     3841 gcatcctgta gcgcgggaaa ctgggcgcga aactgcgcgg gattaaaaac gttcatggta
     3901 ctcctcggct tgatgcaccg atcgtgacgc aaaattcact ttgatacaag gagagtgcta
     3961 accatcagaa ttatctggat atcctggcga agccgcgtca gtaacatatg ctgaatagtg
     4021 ttaggcgtta attttagcct gttaatcaat actattaaga tttattaatc gctttaagga
     4081 gaagatgatg aaaaagactg ccgcaattat ttctgcctgt atgctgactt ttgccctgag
     4141 cgcctgttcc ggttcgaact atgtgatgca caccaatgac ggacgtacca tcgtctctga
     4201 cggcaaacca cagactgata acgataccgg tatgatttcg tataaagacg ctaatggcaa
     4261 caaacagcag atcaaccgta ccgacgtgaa agagatggtc gaactggatc agtaattcgc
     4321 gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct acattaatca ctatggacag
     4381 acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag
     4441 accaattgca aacacaacaa cacaacatca caaccgtaag ccaaaagttc accagaacac
     4501 gcattccgat aaaacttttc gttccggctc aggaagtgcc gccactatag gtatttgctg
     4561 gtagaagctc aacggacaat ttataatggc tcagattaaa aaaactaata ggttacacag
     4621 tgtaatctaa ttgttaaatt catttaacat caaagtttaa aagccatgtc taaacgatta
     4681 ccaccgctaa atgccttacg agtttttgat gccgcagcac gccatttaag tttcactcgc
     4741 gcagcagaag agctttttgt gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag
     4801 gattttctgg ggctaaaact gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa
     4861 gggcaaagct atttcctcga tatcaaagag atattttcgc aattaaccga agcgacgcgt
     4921 aaactccagg cccgtagcgc taagggggcg ttgacggtca gtttactccc cagtttcgcc
     4981 attcattggt tggttccgcg actttccagc tttaattcag cttatccggg aattgatgtt
     5041 cgaatccagg cggttgatcg tcaggaagat aagctggcgg atgatgttga tgtggcgata
     5101 ttttatggtc ggggcaactg gccggggcta cgggtggaaa aactgtacgc cgaatattta
     5161 ttgccggtgt gctcgccgct actgctgact ggcgaaaacc ccttgaagac cccggaagat
     5221 ctggctaaac atacgttatt acatgatgcg tcacgccgtg actggcagac atatacccga
     5281 cagttggggt taaatcatat caacgttcag caagggccaa tttttagcca tagcgccatg
     5341 gtgctgcaag cggctatcca cgggcaggga gtggcgctgg caaataacgt gatggcgcaa
     5401 tctgaaattg aagccggacg tcttgtttgc ccgtttaatg atgttctggt cagtaaaaac
     5461 gctttttatc tggtttgtca tgacagccag gcagaactgg gtaaaatagc cgcctttcgc
     5521 caatggatcc tggcgaaagc cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa
     5581 taatttacgt agggtacgac catgaccagc cgttttatgc tgattttcgc cgccattagc
     5641 ggcttcattt ttgtggctct gggcgctttt ggcgcgcatg tgttaagtaa aaccatgggg
     5701 gccgttgaga tgggctggat ccagaccggc ctcgaatacc aggcgtttca tacgctggcg
     5761 atcttaggtc tggcggtggc aatgcagcgt cgcatcagta tctggtttta ctggagtagc
     5821 gttttcctcg cgttaggcac ggtgctgttc agcggcagcc tttattgcct ggcgctgtcc
     5881 catctgcgtt tgtgggcgtt tgttacaccg gttggcggcg tgagcttcct tgcgggttgg
     5941 gcgttaatgt tagttggtgc tatccgttta aagcgcaagg gcgtaagtca tgaataaggt
     6001 tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc
     6061 cggccagcgg gaaattttcg gttttgcccg cgtgaaagag aatgcgggtt atgtgattta
     6121 tgaatgttat caacctgatg atggcgataa gttaatccgt gagctgccgt tcagttcatt
     6181 aatttttgcc cgccagtggt ttgtagtggg ggaactcctg cagcatttgc cgccagaaga
     6241 tcgtattacc cccattgttg gcatgttgca gggtgtagta gagaagggcg gtgaactgcg
     6301 tgttgaagtt gccgatacca acgaaagcaa agagttgctg aaattctgcc gtaaattcac
     6361 cgtgccgcta cgcgctgcct tgcgtgatgc gggggtgctg gcgaactatg aaacgccgaa
     6421 gcgtccggtt gtgcatgtat tctttattgc accaggctgt tgctataccg gttactcata
     6481 cagcaacaat aattcgccgt tctatatggg cattccgcgc ctgaaatttc cggcagatgc
     6541 gccgagtcgt tccacgctca aactggaaga ggcatttcat gtgtttattc ccgcggatga
     6601 gtgggatgaa cgcctggcga acgggatgtg ggcggtggat ttgggcgctt gcccaggcgg
     6661 ctggacctac caactggtga agcgcaacat gtgggtttat tccgtcgaca acggcccgat
     6721 ggcgcaaagt ctgatggata ccgggcaggt gacgtggctg cgggaagacg gtttcaaatt
     6781 ccgtccgaca cgcagcaata tctcctggat ggtatgcgat atggttgaaa aaccggcgaa
     6841 agttgcggca ttaatggcgc agtggctggt taatggctgg tgccgtgaaa ctatcttcaa
     6901 tctcaaactg ccgatgaaaa aacgctacga agaagtgtca cacaatctgg cgtatattca
     6961 ggcacagctt gatgaacatg gcataaatgc tcagattcag gcgcggcagt tgtatcacga
     7021 tcgcgaagaa gtgacggtgc acgtccgccg tatctgggct gcggtgggtg gtcgtcgcga
     7081 cgagcgataa caggcaaggc cggggcgtcg tctccggcct gctacccttt ccgtcaggct
     7141 gttaccaaag aagttgcaac ctggcgctca tcagaaataa tgtgcgtgac ctcatccaga
     7201 tgcccgatgc gggcttcccc tgaacgatta aatttacttt tatcaatcaa taacaacgat
     7261 tgcgcggcac gttttaataa cattgatttg taatcagcgt tgatggcatt ggagtcccac
     7321 agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa acagatcgat ttccagcgat
     7381 ttaagttggg aaatcagcga gggattgacg taacagccat atttgcgctc aagtgtgccg
     7441 ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt catggcaaat cggatggcta
     7501 ttggtgaaga cctggatgtt gatgtcaggc aactggcgtg ccagatacca gcaggttgaa
     7561 ctggcgtcta aggctatcac catcccttct tcaatccacg cgagcgcctc gcgcgcgata
     7621 tctgctttat gcgcataatg gcttttcagc cggatgtgaa agggatcgcc actgtcttga
     7681 ttttgacggt ggatatattt agcgcgccca tgattgcgca gaattttacc ctgcgtctgt
     7741 aattcattga gatcgcgacg aatggtttct ttactgacct ttagctgttc agagagagct
     7801 tccgtggtca ggctggtatg gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct
     7861 ttcatatttt tcaccccatc attcgataac cgcccgcgtc atgcgggcgt accggtatga
     7921 gattacggtg ttaccccttt ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg
     7981 ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg caaaacgatt gatgcggatg
     8041 atgtcaggac acggggcttg tagtgaaagc gcattacggt aacgtcgttc tacttcagga
     8101 tcgagagtgt caccttctac cgccgccatc atcaccagcg gtggtgcata actgtccagt
     8161 tcaaataacg ggataatcgc ctggagcaag tcgctcacca acaggccatc agcgcggatc
     8221 acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca
     8281 tgccccatct ctgccagcac tttcaatagt tcgggagaaa ttaacggcga aattgttttc
     8341 agcatttcac acctcctcta taaattcagg ttcagtttgc gggtagaaat aacggtactg
     8401 ataatgaatc tgcgcgcggg cttcttccgg gctgttaaat tcccctacgc cataccaacc
     8461 aaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg agaactttta ccggaatatc
     8521 aagcatattg gctttaatct gattccacaa tgtgttgcga cttcctccgc cgactaacaa
     8581 taattcagag gctttaaagt gcccgatttt ttccagcatc tgtagattgc gctgtaattg
     8641 cgtggttaac ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat taagcgttac
     8701 gccttgccag ccagcgttct ggcacgacaa taaatcacac tgcattttca ctccatctgc
     8761 gccaggcgcg atcaggcgag cttcttcaat caacatttgc cagggagttt cagccgtcca
     8821 gaacagtttt ctcacccatt ccagcacgcc ggatgccagc cattgcatac ctgggttata
     8881 caaccctgcc tggctatcca gttcgcaggt agaaccggcg tactgactta acagcgaagt
     8941 atcaacctgg gcgctgcgga ccattaaaat ttcccatgta ccggaagaga gcacgggttc
     9001 attttgttcg gcaccagcgc caaaaagggc gaactgggta tcgtgacctg cggaaatcac
     9061 cggtatgcca acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg taccaatctg
     9121 ttcacccgct tccaccagac gagggaagag tcggcgtgga atactggtgg cttgtaaaat
     9181 ttgcggactg aaatcgcgtt gctggatatc cagcatctgg ctggttccgg ccatcgtaat
     9241 atcagtagtg aattcgccgg ttaaacggtg gttaatcagc gacgaaataa agagccaggc
     9301 gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac cacaccaact tatataacgt
     9361 attgaaacta aaggctccga ctccagaaat agcctgcaac tgctgtgcgg agatcaaccg
     9421 ttcaatattg tccattacca cggctgttcg cggacatttc cagctaatga tcggatagag
     9481 cagattgcct tgcttatcta ccagagcgcc atccacacca aaggtggtga cggcgatacc
     9541 acggatgtgg caatcagtca gttcactatt gatttgccga cagcaatcag caaagcgttg
     9601 caaaatggcg tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc
     9661 attaggcgtt gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac
     9721 attggtcgcg ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat
     9781 atagccggat aacattgctt atccggctaa ccactcttgc ttgttcaggc ccggtagccg
     9841 gagcgaccgg gcatcacatc agggagtcat gtattaacgc ttgtacaacg ggccgtagtt
     9901 ctggcaagcg cggtaatcct ggccttcaat atccatgccg tgcgcggccc aggcagaagg
     9961 acgatacact ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc
    10021 aagagtgata aagtctgcgc caacgtggcc gatggtcaga actccgtggt tagcgcccca
    10081 gttcgccatt accgagtaca catccgtaaa cggcccttta ccggtgaggc gcggtgcaaa
    10141 ccaggtggtt ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg
    10201 caattccacg ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt
    10261 gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc
    10321 gccacggaag tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc
    10381 ctcttgctga gagatttccc agtgcggctt catcgtcggt ttaccttcgc tgtcgcgttg
    10441 tttacaggag ccgtccagcg cagcagaacc ggagttgatc aaatggatga tgccgtgttc
    10501 tgccagcccg tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt
    10561 acggacatcg gcaaatacct gagcggtgcc ggtgagctgg tggcccatta acattgccac
    10621 gccgttaaga ctgtcgtttt cggtcgccac gacaaacggt ttgcgcacgc cattccagtc
    10681 aaatgaactg ttgaggatcg cttcagcggt atcgccattg ggatattgat cggtccagtg
    10741 acgttgcccc tggaagcctg cagcgatggc gttgtagcca agtgattctt ccacgcgacc
    10801 aatatcggcc agtttgctat tgccttgcat catgtcgcgg atacacatcg ccatcagtaa
    10861 actttcgcgc agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc
    10921 atcttcgcca tagcggaagt ttttatcagc ccaggccagc gccatttcca gttcggcttc
    10981 gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt
    11041 cattcccagc caggattcaa agaagttgtg atcaacgatg gaaccggcga tacccatcga
    11101 aacgccgccc agcgacagat aacttttacc tttcatgctg gcgactgcta aaccggcgcg
    11161 ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg
    11221 aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc
    11281 cgctgccagg taaacagcgc cgggacgttc agtgccgtta aagccccaaa tggctttcgg
    11341 gcgggttgga tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat
    11401 ggtgaggcct acattctgac tgctgaattt ttcttcgcaa gcagcggctt cagccatacc
    11461 cgcgatacag gtatcggaaa tgacacactc gacggcagct ccgcaggcat ggcgcagttt
    11521 ctcggtcagc agtgcagccg tggctttcgc catattcatt gtttgttctt caagcgactc
    11581 acgaacaccc atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat
    11641 ttttttcatt actgattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga
    11701 gaacggaaac gggcaaagat aaagatgact gcgaagcaga gtgccgggat cagttcggca
    11761 gtggggatgt tgcccgctgc gtcactgaca aaccccatga ccggagtgac aataccgccg
    11821 ccaataatgg tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta
    11881 atgcccagcg agaagattgt tgggtactga atcgacataa aggcactgca taaagtcagg
    11941 gctattaagc ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt
    12001 aatgcgtagg ctgccaggac tttgtgtggc gcgaagcgac tgatgagcca ggtaccggtg
    12061 aaacgaccaa taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgct
    12121 gtcatacctg gaatttcttc tacagcgtag cgaattaaat agctccagca ggccgtttgt
    12181 gcgccaacgt agcagaattg cgctaatact gcccagcgcc agtggcgaat acgcgccagg
    12241 cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat
    12301 gccgggaatt tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc
    12361 atataaggtg tctgtaccga taataccaag ctgtgtttat acgcactcaa ttgctctgga
    12421 gacattttat cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc
    12481 ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg tttgcgcaag atttaagcgg
    12541 aagtgaccac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc agtttccaga
    12601 caacctaatc cggctgcaat aataaatagg ccaactaaaa acaaggtgta gttcattatt
    12661 tctgcggcgg gccagaataa tgcagcgccg aaggcatata aaaacaatcc ggtaataatc
    12721 cctgctttat aactgagttt tttcatcaat atcccggcag ggattgggat aatgaaataa
    12781 ccaaagtaaa aggccgattg gatcaggcca gcctggaaat ttgtcagcgt aaaagcctgc
    12841 tggaattgag gcaataaaat gtcgttaagg ttattggcta ccgcccaaag aaaaaacagt
    12901 gagcacagca gcgcgaatgg aataatgtaa cttctgcttt gccctgcatc tttatctacc
    12961 gcacggtaac tctgcgtttg tattgatgtg tttcccatag catcctctta ggttcagaag
    13021 cttaagcacg taatcggtta tgttttcaaa agtgcaaaac cagcgagttc gggcttcata
    13081 tcagtgaata tttgcctgtc tgaaatgaac tcatggattc attttcaaat aaaaaacgct
    13141 ctgtaatatg acggcggtca cacttattag ccggagggtg actttatgtg actaccatca
    13201 ctttaataat cgaaatttca tgcccgtttc agaaaataaa tcataaaaaa cacaaaccag
    13261 atgaccgtat taattgtttg tgccgaaaac gggcattcct ggcagggagg a
//
LOCUS       DAPQZD010000132        13284 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPQZD010000132 DAPQZD010000000
VERSION     DAPQZD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.134"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(223..1113)
                     /gene="rarD"
                     /locus_tag="RO454_003675"
     CDS             complement(223..1113)
                     /gene="rarD"
                     /locus_tag="RO454_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDY0935443.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRISK"
     gene            complement(1165..1632)
                     /locus_tag="RO454_003676"
     CDS             complement(1165..1632)
                     /locus_tag="RO454_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDY0935444.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1797..2666
                     /gene="pldA"
                     /locus_tag="RO454_003677"
     CDS             1797..2666
                     /gene="pldA"
                     /locus_tag="RO454_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDY0935445.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2791..4620
                     /gene="recQ"
                     /locus_tag="RO454_003678"
     CDS             2791..4620
                     /gene="recQ"
                     /locus_tag="RO454_003678"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDY0935446.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4685..5305
                     /gene="rhtC"
                     /locus_tag="RO454_003679"
     CDS             4685..5305
                     /gene="rhtC"
                     /locus_tag="RO454_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDY0935447.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5343..6203)
                     /locus_tag="RO454_003680"
     CDS             complement(5343..6203)
                     /locus_tag="RO454_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDY0935448.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6362..6982)
                     /gene="rhtB"
                     /locus_tag="RO454_003681"
     CDS             complement(6362..6982)
                     /gene="rhtB"
                     /locus_tag="RO454_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDY0935449.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7093..8115
                     /gene="pldB"
                     /locus_tag="RO454_003682"
     CDS             7093..8115
                     /gene="pldB"
                     /locus_tag="RO454_003682"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDY0935450.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8123..8923
                     /gene="yigL"
                     /locus_tag="RO454_003683"
     CDS             8123..8923
                     /gene="yigL"
                     /locus_tag="RO454_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDY0935451.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8999..9898
                     /gene="bioP"
                     /locus_tag="RO454_003684"
     CDS             8999..9898
                     /gene="bioP"
                     /locus_tag="RO454_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDY0935452.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9786..10739)
                     /gene="metR"
                     /locus_tag="RO454_003685"
     CDS             complement(9786..10739)
                     /gene="metR"
                     /locus_tag="RO454_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDY0935453.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10857..13118
                     /gene="metE"
                     /locus_tag="RO454_003686"
     CDS             10857..13118
                     /gene="metE"
                     /locus_tag="RO454_003686"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDY0935454.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         3197 a         3311 c         3520 g         3256 t
ORIGIN      
        1 aaaccagccc cggcattgga aaaaaaagaa ataagtaaga tacaaattag atttgtaaaa
       61 ataaatgaaa atatatagcg gcgtctggaa attctgcctt tagatgttgg aataaatata
      121 cgcaacatta attttacatc cttgtgtaag aatttcgtta gcatggcaat attcccgctc
      181 ttatgcaaga gcaggcatat tttttcagaa tatatttatt ttttatttgg aaattctacg
      241 ctgcgtataa atcgcatcca tcacaaaaat tgccagcgcc acccaaataa aggcgaaagt
      301 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
      361 cgtcggacca atgtactgga aaaagcctaa cgttgagaga cgcaggcgtg tcgcagctgc
      421 agtaaaacac aacagtggca cggtggtgac aataccggcg gcgatcagca gtaaattcag
      481 cgacatcggg ttttgcccca tatgactggt tgagctgtcg gcaatagcaa acaggtaaat
      541 cgccgccacg ggcagcaacc acatagtttc gattaacatg ccggtttgcg cttcaacagc
      601 aatcttcttg cgcaccaggc cgtagaaagc aaaactaaac gccagcccca gcgcgatgat
      661 cggcagtgaa ccaaaagtcc acaactgaac caacacgcca caaatcgcca gaatcaccgc
      721 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
      781 cagtgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
      841 gataaacagt agccagttgc caccaatcag cacggcagag actgccagca taaaaatttt
      901 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
      961 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     1021 gtaaatcagt ttgaagtacg ctggcgcaat accccaaata aaataagcgg caagagcgag
     1081 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     1141 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     1201 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     1261 caacaggcta ctggtagcag taaaacgctc tcctctgcct gggcgcagat aatcgacgcg
     1321 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     1381 gtggcgggtt aaggtacttc ccacgcacac cagacctgcg gcgacatcca gcgccgacgc
     1441 aatgaccccg ccgtgtaaaa tgctttgcgc ccagttgccc accatcatcg gctgattttt
     1501 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     1561 aaatggcatg tgataaacaa acatctcacc cactaatttg agggcttgtt cagcggtcag
     1621 tacggcagac ataacatcct tacacttcat tagttaatga aatgttgatt ttatgcttat
     1681 ttattgttgg tttctacttt aggaagggat aactaacggc tatggagtta agtatgtaaa
     1741 atagcccgca gaaaaatatt caccttatca ataattcgtt acggagaaca cgagcgatgc
     1801 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     1861 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     1921 tgctgcagga gcatgacaat ccgttcaccc tttatcctta tgacaccaac tacatcatct
     1981 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaacg
     2041 cgcgtaagga tgaagtaaag ttccagttga gcctggcgtt tccgctgtgg cgtgggattt
     2101 tggggccaaa ctctgtactg ggcgcctcct acacacagaa atcctggtgg cagctttcta
     2161 atagcgatga gtcatcaccg tttcgtgaaa ctaactacga accgcaattg ttcctcggtt
     2221 ttgccacgga ttacaacttt gctggctgga cgctgcgcga tgtggaaatg ggctacaacc
     2281 acgactcaaa cgggcgttca gacccgactt cccgcagctg gaaccgcctt tatacccgcc
     2341 tgatggcaga aaacggtaac tggttggtag aggtgaagcc gtggtatgtg gtggggaata
     2401 cggacgataa cccggacatc accaaatata tgggttacta ccagcttaaa atcggctatc
     2461 acctcggtga tgcggtactg agcgcgaaag ggcagtacaa ctggaacacc ggttacggcg
     2521 gcgcggagtt aggcttaagc tacccgatca ccaaacatgt gcgcctttat actcaggttt
     2581 acagcggtta cggcgaatcg ctcatcgact ataacttcaa ccagacccgc gttggtgtgg
     2641 gagttatgct aaacgacctg ttttgatgaa tggttggcaa atctggaatc cagcatccag
     2701 gattaccctc tcagagacta aaagcattgc agtttctcgc gcaggcgctg aaaatagcgc
     2761 ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg cggaagtgtt gaatctggag
     2821 tccggagcta aacaggtttt acaagaaacc tttggctacc aacagtttcg ccccggccag
     2881 gaagagatta tcgacaccgt actttccggt cgcgattgcc tggtcgtcat gcccactggt
     2941 ggcggaaaat ccctttgcta tcaaatccct gccttattgc taaacggcct taccgtggtt
     3001 gtctcaccgc tgatttcgtt gatgaaagat caggtagatc aactgcaagc caacggcgtg
     3061 gcggcggcgt gccttaactc gacgcaaacc cgcgagcagc aacttgaagt gatgacaggc
     3121 tgtcgcaccg ggcaaatcag cctgctttat atcgcgccgg aacgcctgat gctggataac
     3181 tttcttgagc atctggcgca ctggaatccg gtgttattag ccgttgatga ggcgcactgt
     3241 atctcccaat ggggtcacga tttccgcccg gaatacgctg ccctcggtca gctgcgtcag
     3301 cgttttccga cgctgccgtt tatggcgctg actgccacag ccgatgacac cacgcgccag
     3361 gatatcgtgc gcctgctggg gctgaacgat ccgctgattc aaatcagcag ttttgaccgt
     3421 ccgaatattc gctacatgct gatggagaaa ttcaaaccgc tcgatcagtt gatgcgttat
     3481 gtacaggaac aacgcggtaa gtcggggatt atttactgta atagtcgtgc gaaagtagaa
     3541 gacaccgctg cgcgcctgca aagcaaggga attagcgcgg cggcctatca tgccgggctg
     3601 gaaaataatg ttcgcgctga tgtgcaggaa aaattccagc gcgatgacct gcaaattgtg
     3661 gtggcgacgg tggcgttcgg catgggcatc aacaagccta acgttcgctt cgtggtgcat
     3721 tttgatattc cgcgcaatat cgaatcctat tatcaggaaa ctggacgcgc cgggcgtgat
     3781 ggcctgcccg cggaagcgat gctgttttac gatccggctg atatggcgtg gctgcgccgt
     3841 tgtctggaag agaagccgca ggggcagttg caggatatcg aacgtcataa actcaatgcg
     3901 atgggcgcgt ttgctgaagc gcaaacttgc cgtcgtttgg tattgctgaa ctattttggc
     3961 gaagggcgtc aggagccgtg cgggaactgc gatatctgcc tcgatccgcc aaaacagtac
     4021 gatggttcaa ccgatgcgca gattgccctt tccaccattg gtcgtgtgaa tcagcggttt
     4081 gggatgggtt atgtggtgga agtgattcgc ggtgctaata atcagcgtat ccgcgactat
     4141 ggtcatgaca aactgaaagt ctatggcatg ggccgtgata aaagccatga acattgggtg
     4201 agcgtgatcc gccagctgat tcacctcggc ctggtgacgc aaaatattgc ccagcattct
     4261 gccctacaac tgacagaggc tgcgcgtccg gtgctgcgcg gcgaatcctc tttgcaactt
     4321 gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc agaaatcgtt cggcggcaac
     4381 tatgatcgca aactgttcgc caaattacgc aaactgcgta aatcgattgc cgatgaaagc
     4441 aatgtcccgc cgtacgtggt gtttaacgac gctaccttga ttgagatggc tgaacagatg
     4501 ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg gtatgcgcaa gctggaacgc
     4561 tttggcaaac cgtttatggc gctgattcgt gcgcatgtcg atggtgacga cgaagagtag
     4621 tcagcagcat aaaaaagtgc caggatgaag actccgtaaa cgtttccccc cgcgagtcta
     4681 atgtatgttg atgttattcc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc
     4741 cggtcccgat ttcttttttg tatctcagac cgctgttagt cgttcccgta aagaagcgat
     4801 gatgggcgtg ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgttgcttgg
     4861 cctacatttg attatcgaaa aaatggcctg gctgcatacg ctgattatgg tgggcggtgg
     4921 cctgtatctc tgctggatgg gttaccagat gctacgtggt gcactgaaaa aagaggttgt
     4981 ttctgcccct gcgccacaag tcgaactggc gaaaagcggg cgcagttttc ttaagggctt
     5041 actgaccaat ctcgcgaatc ctaaagcgat tatctacttt ggttcggtgt tctcgctgtt
     5101 tgtcggtgat aatgtcggca ccaccgaacg ctggggcatt ttcgcgttga ttattgtcga
     5161 aacactggcg tggtttaccg tcgttgccag cctgtttgct ctgccgcaaa tgcgctgtgg
     5221 ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg
     5281 cattcatttg attatttcgc gttaagcgct tcccccctga tacgctatca gggggaataa
     5341 catcaggatt gcaattgata aaggtcttcc tgagaaagcc cggtcatttc cataatcaac
     5401 tgcaaatcaa agccacgctg tagcatggct tttgcgacat tgagaagggc atcgcgccga
     5461 agttcttccg ctaaagtcat cagttcctct ccttgttgag gcactcgttg tgccagacca
     5521 tgtactaatg ttcgtacatc ctgtgcttca cctaccagcg ccatgtagtt tatcaacatg
     5581 cgtatttgct gtccgctcat ctgccccaga atcattaagc gggataactt atctaacagc
     5641 gttgtcatat cccgttgacg gatatgcttc tgtaccagcg taaaggcggc catgctgcgg
     5701 tgttgcatga tttcgtcatc aggaattagc gtaatatcaa ccagcggaaa agcatgaaaa
     5761 tagagatcgc gtgcaatgtc cggatccgga aaaccatcga gccagtttgt tgaccatgga
     5821 tatgggctac gctttccctg gtaaaaaaga atggggatca ccagaggcag ataatcatgt
     5881 cccgcttcaa gatgccgctg catggtggca atagcatagc gaagcatacg gaacgccata
     5941 ttttcatcag gtgaactttg atgctcaatc aacacataga tatatccgca ccctttcgtc
     6001 gtctctactg aataaagtac gtcgttgtaa tactggcgta ggttttcctc tacaaaactg
     6061 ctggactcga gatggagcgt atcaagattg cagagtttaa ctaaagaagg cggcagatga
     6121 atttcaataa agtcacgggc cgtttctggt cgggataaaa aagccttaaa gattgcgtca
     6181 tgtggtgtcg ttgcttccgt catttttcat catccgtggg tgaaatatgg cggcattctg
     6241 gcactaacaa agagaagtaa atagtaaata aattaacaga ttacgcttat tacgagatgc
     6301 ctcgagcgcg gatgtaacaa tctctcgagg cacattaatt tacgtgagct ttattccagt
     6361 tttaagcatg cctcgccgat gctaacagcg ctcccaccag cataaataac gagccgaaaa
     6421 tcttattcag cgccttcatc tgctttggtc ctttaatcca tagagcaatc cgttgagcaa
     6481 gggtggcgta accgatcatc acaataatat cgaccacaat agtggtgacg ccgagcacga
     6541 tatactgcat cagctgcggc tgttgcggca tgatgaattg cggaaatagc gccgccagaa
     6601 acacaatact tttgggattg gtgagattga caaaaactgc gcgctggaac aaatgtcgac
     6661 gcgattgagt agaggccagc gatttaagat caattgcgcc agcggcgcgc cactgctgga
     6721 tcccaagcca aatcaagtaa gctgcgcctg cccacttcaa cacttcaaac gcaatcactg
     6781 agcgggaaaa tagcgtcccc aaccccacgc caaccagcac aatatgaatc gccagtccgg
     6841 tctgaagccc agcaatagac gccaccgcgc cgcgataacc gtggttgagc gaggtggtca
     6901 tggtgttgat tgcgccagag cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg
     6961 caaaccacca ttctaaggtc atgatgaact cccggtgtgt ctatttttgt gccacaatac
     7021 gctactgtcg cagcgttgtg tcaggcacgc ttaaaaaaac gattttacgt ggtttaagag
     7081 gcagattacc cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa cgcgtttgct
     7141 gcttttacca tgggaccgct gactgatttc tggcgtcagc gtgatgaagc agagtttact
     7201 ggtgtggatg acattccggt tcgctttgtc cgttttcgcg cacagcacca tgaccgggtg
     7261 gtagttatct gcccggggcg tattgagagc tacgtaaaat atgcggaact ggcctacgac
     7321 ctgttccatt cggggtttga tgttttaatc atcgaccatc gcgggcaggg acgttccggt
     7381 cgcctgttag ccgatccaca tctcgggcat gttaatcgct ttaatgatta tgttgatgat
     7441 ctggcggcat tctggcaaca ggaggttcag cccggtccgt ggcgtaaacg ctatatactg
     7501 gcacattcga tgggcggtgc gatctccaca ttatttctgc aacgccatcc aggtgtatgt
     7561 gacgccattg cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc gtcatttatg
     7621 gcacggcaga tcctcaattg ggccgaagcg catccacgtt tccgtgatgg ctatgcaata
     7681 ggcaccgggc gctggcgcgc gttgccgttt gctatcaacg tactgaccca cagcagacag
     7741 cgatatcgac gtaacttacg tttctatgct gatgacccaa cgattcgcgt cggtgggccg
     7801 acctaccatt gggtacgcga aagtattctg gctggcgaac aggtgttagc cggtgcgggt
     7861 gatgacgcca cgccaacgct tctcttacaa gctgaagagg aacgcgtggt ggataaccgc
     7921 atgcacgacc gtttttgtga actacgcacc gctgcgggcc atcctgtcga aggaggacgg
     7981 ccgttggtaa ttaaaggtgc gtaccatgag atcctttttg aaaaggacgc aatgcgctca
     8041 gtcgcgctcc acgctatcgt tgattttttc aacaggcata actcacccag cggaaaccgc
     8101 tctacagagg tttaaatttc ttatgtacca ggttgttgcg tctgatttag atggcacgtt
     8161 actttctccc gaccatacgt tatcccctta cgccaaagaa actctgaagc tgctcaccgc
     8221 gcgcggcatc aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg ggcaaattcg
     8281 cgataatctg gagattaagt cttacatgat tacctccaat ggcgcgcgcg ttcacgatct
     8341 ggatggcaac ctgatttttg ctcataacct ggatcgcgac attgccagcg atctgtttgg
     8401 cgtagtcaac gacaatccgg acatcattac taacgtttat cgcgacgacg aatggtttat
     8461 gaatcgccat cgcccggaag agatgcgctt tttcaaagaa gcggtgttca attatgcgct
     8521 gtatgagccg ggattgctgg agccggaagg cgtcagcaaa gtgttcttca cctgcgattc
     8581 ccatgaaaaa cttctgccac tggagcaagc gattaacgct cgctggggcg atcgcgtcaa
     8641 tgtcagtttc tctaccttaa cctgcctgga agtcatggct ggcggcgttt caaaaggcca
     8701 tgccctggaa gcggtagcga agaaactggg ttacagcctg caagattgta ttgcctttgg
     8761 cgatggaatg aacgatgccg aaatgctgtc gatggcgggg aaaggctgca ttatgggcag
     8821 cgcgcatcaa cggctgaaag atctgcatcc agagctggaa gtgattggta ctaacgccga
     8881 agacgcagtg ccgcattatc tgcgtaagct ctatttatcg taatcgttct ttatttggtc
     8941 agttgtcaac ctgatacttc gctacaatgg atactcgtta atcaaagagt tttccattgt
     9001 ggcgctactt atcatcacca cgattctgtg ggccttctcc tttagctttt atggcgagta
     9061 ccttgcgggg cacgtcgata gctattttgc ggtgctggtg cgcgttggtc tggcggcact
     9121 tgtttttctg ccgtttctgc gtacccgtgg caatagcctg aaaacggtcg gcctgtatat
     9181 gctggtgggc gcgatgcagc tcggtgtgat gtatatgctg agtttccgcg cctatctcta
     9241 cctgacggtt tctgaactgc tgttgttcac cgtactgacg ccgctctaca tcacgctgat
     9301 ttatgacatc atgagtaagc gccgtctgcg ctggggctat gcttttagcg ccttgttggc
     9361 ggtgattggt gccgggatta ttcgctatga tcaggtcacc gaccatttct ggactggctt
     9421 gttgctggtg caactctcca atatcacttt tgccatcggc atggtgggtt acaaacgtct
     9481 gatggaaact cgcccgatgc cgcagcataa cgcctttgcg tggttctatc ttggcgcgtt
     9541 cctggtggca gtgattgcat ggttcttgct gggaaatgcg cagaaaatgc cgcaaaccac
     9601 gttgcaatgg ggcattctgg tgtttcttgg cgtggtggct tccgggattg gctactttat
     9661 gtggaactac ggcgcgacgc aggtggacgc cggaacgctg ggcattatga ataatatgca
     9721 cgttccggca gggctgctgg taaacctggc tatctggcac caacagccgc actggccaac
     9781 gtttattaca ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta agtgggtcgc
     9841 tccgcgctct tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga acgaataaac
     9901 gcttctgtaa tcggctgacg ctgctcgcca tcgcgcacgg cggcgtacag tcggctccac
     9961 aagccttcgc ccagggtttt ggtgaccacc agaccctggc gttcaaaact ctccaccacc
    10021 caatgaggta gtgcggcaat acccatccgt gcggcaacca tctgaatcaa caataaggtg
    10081 ttatcgacgc ttttcagtga cgggctgacg cctgctggct gaagaaaatg ccgccagaca
    10141 tccagtcggc tacgctgcac cggataaatc aacagcgttt cgctggcgag atcttccggt
    10201 gtaatacgcg tttttgccgc cagtggatgg tcaggtgcta acaccagacg tacttcatag
    10261 tcgaacatcg gcgaataatg caggccactg cgcggcagaa tatcggacgt catcaccaga
    10321 tccagctcgc cctgttgcaa cgcaggctgc ggatcaaacg ttacgcccga tttaaaatcc
    10381 atctccacct gcggccagtt cttatggaaa ttttctaatg cgggcgtcag ccactgaata
    10441 cagctatggc actctatggc aatgcgcagg cgcgtctgct gcggttcatt gcaggcttgc
    10501 agtgcctggc taatttgcgg cagcacctga tttgccagtt gcaacaggat ttctccctgc
    10561 ggtgtaaagc gaagcggctg gcttttacgc acaaatagcc ggaagccaag gcgttgttcc
    10621 agatcgctaa actggtgaga cagggcggat tgcgtttgat gcaacgtcgc cgcagcggct
    10681 gcgagtgagc cgcagttccg caacgcttgt agcgttttca ggtgttttac ttcgatcatg
    10741 aaagtccttc acttcggcat gaataatttg cgcttgagga atatacagta accgccaatt
    10801 atggatgtgt aaacatctgg acggctaaat actaaaaatt ccataagggg catataatga
    10861 caattcttaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag
    10921 cgcaagagag ttattgggcg gggaactcca cgcgtgaaga actgctggcg gtagggcgtg
    10981 aattgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg
    11041 gcgattttgc ctggtacgat catgtactga caaccagtct gctgctgggt aatgttccgc
    11101 aacgtcatca gaacaacgat ggttcggtag atatcgatac cctgttccgt attggtcgtg
    11161 gacgtgcacc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg tttaacacca
    11221 actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg acctggacgc
    11281 agctgctgga ggaagtggac gaggcgctgg cgctgggcca caaggtgaaa cctgtgctgc
    11341 tggggccggt tacctacctg tggctgggta aagtgaaagg tgaacagttt gatcgcctga
    11401 gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaacta gcgaaacgcg
    11461 gcattgagtg ggtacagatc gacgaacccg cgctagtgct ggaactgccg caggcgtggc
    11521 tggatgcata caaacccgct tacgacgcgc tccagggcca ggtgaaactg ctgctgacca
    11581 cctattttga aggcgtaacg ccaaacctcg acacgattac tgcgctgcct gttcagggtc
    11641 tgcatgtcga tcttgtacat ggtaaagatg acgttgctga actgcacaag cgcctgcctt
    11701 ctgactggct gctgtctgcg ggtcttatca atggtcgtaa cgtctggcgc gccgatctta
    11761 ccgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg gtggcatctt
    11821 cctgctcact gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag
    11881 tgaaaagctg gtttgccttc gccctgcaaa aatgtcatga actggcattg ctgcgcgatg
    11941 cgctgaacag tggtgatacg gcagctctgg cagagtggag cgctccgatt caggcgcgtc
    12001 gtcactctac tcgtgtacat aatccggcag tagaaaagcg tctggcggcg atcaccgccc
    12061 aggacagtca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt gcgcgtttta
    12121 aactgcccgc gtggccgacc accacgattg gttccttccc gcaaaccacg gagattcgta
    12181 ccctgcgtct ggattttaaa aagggtaata tcgacgccaa taactaccgc acgggcattg
    12241 cggaacatat caagcaggcc attgttgagc aggaacgttt gggactggat gtgctggtac
    12301 atggcgaggc cgagcgtaat gacatggtgg aatactttgg cgagcatctg gatggctttg
    12361 tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag ccaccgattg
    12421 ttattggtga cgttagccgc ccggcaccga ttaccgtgga gtgggcaaaa tatgcgcaat
    12481 ccctgactga taaaccggtg aaagggatgt tgactggccc ggtgactatt ctctgctggt
    12541 cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acagattgcg ctggcactgc
    12601 gtgatgaagt ggccgatctg gaagccgctg gaattggcat cattcagatt gacgaaccgg
    12661 cattgcgcga aggtttacca ctgcgtcgca gcgactggga tgcctatctc cagtggggcg
    12721 tggaggcttt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc cacactcaca
    12781 tgtgttactg cgagttcaac gacattatgg attcgattgc ggcgttggac gcagacgtca
    12841 tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa gagtttgatt
    12901 atccaaatga aatcggtcct ggcgtctatg acattcactc gccaaacgta ccgagcgtgg
    12961 aatggattga agccttgctg aagaaagcgg caaaacgcat tccggcagag cgtctgtggg
    13021 tcaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg gcactggcga
    13081 acatggtgca ggcggcgcag aatttgcgtc gggggtaaaa tccaaaccgg gtggtaatac
    13141 cacccggtct tttctcatta cagcgacgtc ttcccagcat actgcgtaaa ccattccaga
    13201 tatcagttca taccctgttt aagaaaatct tctttattcc cttctcatga aataaatttc
    13261 gtcatttccg ccattttttc tatc
//
LOCUS       DAPQZD010000133        13255 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPQZD010000133 DAPQZD010000000
VERSION     DAPQZD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.135"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            24..774
                     /locus_tag="RO454_003687"
                     /pseudo
     CDS             24..774
                     /locus_tag="RO454_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(915..1688)
                     /gene="uxuR"
                     /locus_tag="RO454_003688"
     CDS             complement(915..1688)
                     /gene="uxuR"
                     /locus_tag="RO454_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDY0935455.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1903..3363)
                     /locus_tag="RO454_003689"
     CDS             complement(1903..3363)
                     /locus_tag="RO454_003689"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDY0935456.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3444..4628)
                     /gene="uxuA"
                     /locus_tag="RO454_003690"
     CDS             complement(3444..4628)
                     /gene="uxuA"
                     /locus_tag="RO454_003690"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDY0935457.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4968..6311
                     /gene="gntP"
                     /locus_tag="RO454_003691"
     CDS             4968..6311
                     /gene="gntP"
                     /locus_tag="RO454_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDY0935458.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6485..7387)
                     /gene="fimH"
                     /locus_tag="RO454_003692"
     CDS             complement(6485..7387)
                     /gene="fimH"
                     /locus_tag="RO454_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDY0935459.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSAHDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7407..7910)
                     /gene="fimG"
                     /locus_tag="RO454_003693"
     CDS             complement(7407..7910)
                     /gene="fimG"
                     /locus_tag="RO454_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDY0935460.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATFDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7923..8453)
                     /gene="fimF"
                     /locus_tag="RO454_003694"
     CDS             complement(7923..8453)
                     /gene="fimF"
                     /locus_tag="RO454_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDY0935461.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNDIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8463..11099)
                     /gene="fimD"
                     /locus_tag="RO454_003695"
     CDS             complement(8463..11099)
                     /gene="fimD"
                     /locus_tag="RO454_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDY0935462.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11166..11891)
                     /gene="fimC"
                     /locus_tag="RO454_003696"
     CDS             complement(11166..11891)
                     /gene="fimC"
                     /locus_tag="RO454_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDY0935463.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11928..12467)
                     /locus_tag="RO454_003697"
     CDS             complement(11928..12467)
                     /locus_tag="RO454_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0935464.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12532..13080)
                     /gene="fimA"
                     /locus_tag="RO454_003698"
     CDS             complement(12532..13080)
                     /gene="fimA"
                     /locus_tag="RO454_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDY0935465.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3212 a         3442 c         3204 g         3397 t
ORIGIN      
        1 taagcaatgc ccttcagagt taaattatta cagataatca cttccttcat catccaaaag
       61 tcgacagtga gttaacacgt aaacatgaac gtgcacgcat ggatacggaa aatatctatc
      121 ttccgcctct tgctcgtggc aataaccata attacgatgg aaaaagcgtt gtcgaaatta
      181 gaaaacttga tattagtaaa gagtcctggc catttaatta tgtgacagaa acatgcagag
      241 aattcaatgg tattacaaca acaggacgta tgctctatcg aaacttgaaa atcacctcag
      301 ctttagacga aatatatggt ggtatatgta aaaaggcgca tgctgccaca gagttagcaa
      361 aaggattacg gctaaattta tttatgaaag gccctttcga tcctgttgag gactatacag
      421 ttcatgaaat aacattaggc cctggttgca atgttcctgg ttatgccgga accacgatag
      481 gatatatttc aacccttcca gcttctcagg caaaaagatg gaccaatgag cagccaagga
      541 ttgatatcta tattgatcag ataatgacgg taacaggggt agcaaattct tccggattcg
      601 cccttgccgc attacttaat gctaatattg agttgggtaa tgacccaata attggtatag
      661 aagcctatcc cgggtgatga aaatgcacca ctcaaacgta tgactctgca accgtcatct
      721 cttccagaac tgtttgaatt aaaaaatggt gaatggaatt atattggtaa ataactacca
      781 ggaagaatga gtactaacat tcaggttata cagatagcta aacaatcccc tgcaatttac
      841 aaagtaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg
      901 cttattataa taagtcaggc gtcgacttta tccagcggcc atgaatcaaa cagatagcca
      961 tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac gttttccaga
     1021 tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc
     1081 tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac
     1141 tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaacag ctccaccagc
     1201 atgctgttat gcgttgcttc ggcaatagcg agatggaact gcatgtcacc gctttcgctg
     1261 ctgcccggcg cactggaagc cagttcacgc tcttccagtt gcaatgcctg acgcattttg
     1321 acgatatctt cgcgggtagc ctgcagggcg gcaaactcgg cgatgttgct ctcaagaagc
     1381 tgtcgcgcct gcaacagctc aaaaggaccc gcatcattgc aaacgttggc atccggactg
     1441 tctgtgttgt ggctgcctga gctatcaaga acatagatac cggcaccccg gcgcacttcc
     1501 accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca
     1561 agcatttctg caatttcacg ctccggcggc aaccgttcgc caggattgta cggcgtcttt
     1621 atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtggca
     1681 gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg
     1741 aacgccttat ccggcctaca acgggcaatt ttacggcacc cgttaatctc aaactatacc
     1801 attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat
     1861 atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg cagccacaca
     1921 ctcgcgcgca ccgcgttcgc acagctgctg atatgccgcc gtcaccgcgc caacaaagtc
     1981 ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg ctttcacgcg
     2041 gtctgcgccc tgatactgcg cgttaatctt ctggaactcg gccagcatcg ggtcgaccac
     2101 gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc cagccacgcc
     2161 cagcgccagg tgacgccagc tgccgccgtt ttgcaggtgc agacgcaccg gatccagcag
     2221 acgctgcggt aacttctggc tgccgtccat cgcaatctgc caggtacggt gacgcagaga
     2281 cgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtccg taccttccgg
     2341 catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt tgcgataatc
     2401 cgggttagtc atggtgtcgg caatagtttc atagccgccg aggtaaccga ggtatgccag
     2461 gaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca caacgtctgc
     2521 aacgaactgt gcgcctactt tatcccagtc cggacgacca ttaacgaagt tatcttcaat
     2581 cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcataaacac ccagttggtc
     2641 agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca
     2701 cgggaaggtg acgttctctt caatccatgc cgccagctgc gggtcacgcg cctgagccag
     2761 ccccagtacc gcgaccttcg ccacatgacc gttttcacgc acgttatcgc aggacatcac
     2821 cgtaaacgct ttcagccctt tttcacgacg cagacgcagg gcttcgacga tgtaaccaat
     2881 cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagcgggt tattgagatc
     2941 cagttgaccg cttgccgcat cagcgcagta gcctttttcc gtgaccgtca gagagacaat
     3001 cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg cagccgtcga tttccgggtg
     3061 cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctttccgcgc ctttttccgc
     3121 cacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt cgttgcctgg
     3181 catcaggtta acttcgcaga taccccagtc gctgtcggtg ctttccagca gatgatgggt
     3241 gtacagcgcc tggtgcgcac ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc
     3301 cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa caatagtagt
     3361 catcgtaaac tccagaatcc gtcttcagag agtgacggca aggaagaagg aaccggcacg
     3421 gcgcgcagcc atgccggtgg atattaacgg ctaaagaaag cgcgctggat cgccagttcg
     3481 acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc tgggttggtt
     3541 ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg gatcaggtct
     3601 tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac cacttcgtac
     3661 atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc
     3721 atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat catatcaacc
     3781 agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtgaaacc gttcgccatg
     3841 ctatttacgg tatcaaccat ccactgcata tcttcaatgg tagaaacaat gcgcggcagg
     3901 ccgaggattg ggcgcggcgg atcgtccggg tgaacagcca tacgcacgcc aacttcttcg
     3961 gcaaccggaa taatcgcctt caggaagacg gcaaagtttt cgcgcagttt ggctttgtcg
     4021 atatctttgt acagctccag gtgcttacgg aactggtcga gggtgtaccc ttcttccgcg
     4081 cccggcagac cggcaatgat gttacgggtc agacgcgctt tgtcttcgtc gctcatagtg
     4141 gcgaagcgtt cagctgcctg agcaatttct tcttcggtgt aatccgcttc cgcacctggg
     4201 cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg cagagctttg
     4261 gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag
     4321 ttgtagcaca cggtgcgaat accgcactgc gccaggttgc gcagagtctg ctgatagtta
     4381 gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat tggcacgctt
     4441 tctacgacag accacaccag gcctgcatct tcaacgatag ccttgcgttt gaggatctct
     4501 tcaacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac gccagttgcg
     4561 cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc
     4621 tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac gcggaaccct
     4681 ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg catcggatga
     4741 gatggcgtat aagttctacc ttttggtaat acatgtcaac ccgaattcta aaattggtta
     4801 accacatcac aagaatttca ctctcctggt tgttgctgca aattgcagca aagggtaatc
     4861 gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca atttacataa
     4921 catgtcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg catgtgctta
     4981 acattctctg ggtggtattc ggcattggtc tgatgctggt actgaatttg aagttcaaaa
     5041 tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg
     5101 atttgatgtc gctgctgcac accatgaaag cgggcttcgg caacacgctg ggggaactgg
     5161 ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc
     5221 accagatagc acatacgctg ctggcgcgtc tcggtctgcg ctatgtacag ctgtcggtga
     5281 ttatcatcgg cctgattttt ggtctggcga tgttctatga agtggccttt atcatgttag
     5341 cgccgctggt tattgtcatt gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc
     5401 cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg ggtcctgtgg
     5461 cgctggtgaa tgcttatggc gcagatatgg ggatggttta tatctatggc gtactggtga
     5521 cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa gttcctcggc aatcttgagc
     5581 gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaacaat ctgccctctt
     5641 tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct accaccatcg
     5701 ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt atcggttcct
     5761 cgccgattgc aatgtttatt gcgatggtgg ttgcattcgt actctttggc accgcacgtg
     5821 gtcatgacat gcagtgggtg atgaacgctt ttgaaagtgc ggtgaaaagc attgcaatgg
     5881 tgattctgat catcggtgcg ggtggcgtgt tgaagcaaac tatcatcgac accggcattg
     5941 gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatt atggcatggc
     6001 tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt cgtttcggcg atgaccgccg
     6061 ccgggattat cagtgctgca atccttgatc cagcaactgg tcagctggtt ggcgtgaatc
     6121 cggcgctgct ggtactggcg acggctgcgg gttccaacac cctcacccac attaacgatg
     6181 catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg ttgaaaacct
     6241 ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgttg attatcagca
     6301 tggtagcgta aaagagcacc tgagcctgcc attggcaggc tctgttcgga ttgtcggcaa
     6361 agtgcacttt gtttatgcca gatgcgacgc tggcgcgtct tatctggcct acaaagggct
     6421 aacgtgcagt tttttagctt caggcaatat tgcgtacctg cattagcaat gttctgtgat
     6481 ttctttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct
     6541 gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gtccctactg
     6601 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
     6661 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
     6721 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
     6781 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcatga gcagaaacat
     6841 cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
     6901 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
     6961 ctttaatcgc caccccaccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
     7021 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtagtc ggaaatggat
     7081 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
     7141 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
     7201 tttgcgtcga aagatctacg accaggtttt gccccacatt cacggcaggc gcaaggttta
     7261 cataaacatt agcgctgcca ccgccaatag gaatagcggt accattggcg gttttacagg
     7321 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
     7381 gtttcatgac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
     7441 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
     7501 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg
     7561 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     7621 tatccggtac tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     7681 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
     7741 ctgaaagaat aaagatcgcc gagatcaaac gtggcattgg tggtggaaac cgtgcacggt
     7801 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
     7861 gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
     7921 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
     7981 acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg
     8041 gtcgtccacg aaagcgcgga cgatggagca ttaaggggta tttgattttg ctgctcattc
     8101 agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg
     8161 gcattgtggc tatctgcaac accggtaaac ccaactttta cggcagaaac ggcattacca
     8221 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gtcgttaaat
     8281 tgcttcgccg cgttttccat caaatcaaca gtaaaattgg ttgattcagc ggccacacta
     8341 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     8401 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     8461 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     8521 tgataattgg cgatacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     8581 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     8641 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
     8701 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     8761 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
     8821 agcgccactc tgttttcccg atattcggtg gcataaggca gcacggcata accgcgccag
     8881 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     8941 agtaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
     9001 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     9061 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctatttcca
     9121 tcgcctcccc cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
     9181 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag
     9241 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     9301 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
     9361 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc
     9421 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
     9481 gagcgcccga gttgctgagt aacggtgagt tgtaatttcc cgcgtttgtt ataagcgagg
     9541 ttgtaatagt cggtgaattt cggcttaacc tgaataacac catcctgtgt ttcgatgttg
     9601 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
     9661 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa
     9721 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
     9781 tccacagaca gagcacccag cgcccccatg tttttcccga taccgaaatt aaaagcacga
     9841 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
     9901 aatgtactct ggaaaaagcg gggtttttcc tgttgcgcat ttccactacg gtattctcct
     9961 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
    10021 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
    10081 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
    10141 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
    10201 accggggcaa atcctctttg actatcgggt aacatattgt catctgaggc caattgtgcg
    10261 ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc
    10321 cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt
    10381 ttgctacctg atgatctgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc
    10441 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
    10501 atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
    10561 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
    10621 atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc ctggaccatt
    10681 gtggttaatg gcacacaggc atcatccgcc agcagattca taccggcgac agaagccata
    10741 ttcagcccca tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
    10801 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
    10861 acgcgatacg tccctggcgg taattcttgt ccattttcaa aacgcgataa atcagccaca
    10921 gcctggggat catccgctaa aaagcgcggg ttaaaataga gttcggcaga tgacaaaggt
    10981 gcctgtgcgg caaaagcaca ggcaacaaag agccggacaa aaaaaccagc caaacgatgc
    11041 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
    11101 attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga aaaattcccc
    11161 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
    11221 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
    11281 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa
    11341 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
    11401 gcggcctgat cgggtggcaa cgctaattta gccggacgat agtacagttt aatgcggctg
    11461 ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
    11521 ttaacgttca tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca
    11581 agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata
    11641 aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa ataggtgcta
    11701 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
    11761 cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
    11821 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt
    11881 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
    11941 aaagagaacc aggcctgtgc attggcgatg ccgccagtaa cccgacgccc ggtagcacga
    12001 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga
    12061 ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg
    12121 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
    12181 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
    12241 aaaggcaccg gtgcgctatc ttcccctacc gcgtgaaacc ggttactgct gatttgcccc
    12301 atattgaccg tcatttgttt atcaccagct tcaatccggc aagtttccgc aataatgacg
    12361 ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc ggccagagca
    12421 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
    12481 acacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
    12541 ctgaaccttg aaggtcgcat ccgcattagc agcacccggg gttgcggcac cggttgcaaa
    12601 ataacgcgcc tggaacggaa tggtgttggt tccgttattc agggttgttt ctgaactaaa
    12661 tgtcgcaccg tccagcgcca gcgcagcacc cgttctgtcc aggatctgca caccaacgtt
    12721 tgttgcgcta cccgcagctg aactctgcag agccagtaca tcagtatgcg tagcatcaat
    12781 tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc aatcattcag
    12841 ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgcttcaggc tagcggtacg
    12901 aacctggcct aactgaacgg tttgatcaac agagcctgca tcaactgcgc aagcggcgtt
    12961 aacaacttcc cctttaaagt gaacggtccc accatttacc gtcgtagtat cggccagagc
    13021 cgctgcggaa ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat
    13081 gctgctttcc tttaaaaaac tatttctaaa tcgacatggg cagtcgttct gtacactttg
    13141 ttttgtcaac gagtttgact gccaacactg cacagttttc ccccaaaaga tgaaacattt
    13201 ggggccattt tgactcatag aggaaagcat cgcggacaaa ctttttcagt ttatt
//
LOCUS       DAPQZD010000134        13206 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPQZD010000134 DAPQZD010000000
VERSION     DAPQZD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.136"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            13..282
                     /locus_tag="RO454_003699"
     CDS             13..282
                     /locus_tag="RO454_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0935466.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            397..567
                     /locus_tag="RO454_003700"
     CDS             397..567
                     /locus_tag="RO454_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935467.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(694..1251)
                     /locus_tag="RO454_003701"
     CDS             complement(694..1251)
                     /locus_tag="RO454_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDY0935468.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1248..1523)
                     /locus_tag="RO454_003702"
     CDS             complement(1248..1523)
                     /locus_tag="RO454_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDY0935469.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1708..1848
                     /locus_tag="RO454_003703"
                     /pseudo
     CDS             1708..1848
                     /locus_tag="RO454_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            complement(1899..2705)
                     /gene="trpA"
                     /locus_tag="RO454_003704"
     CDS             complement(1899..2705)
                     /gene="trpA"
                     /locus_tag="RO454_003704"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDY0935470.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPVGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKATTRR"
     gene            complement(2705..3898)
                     /gene="trpB"
                     /locus_tag="RO454_003705"
     CDS             complement(2705..3898)
                     /gene="trpB"
                     /locus_tag="RO454_003705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDY0935471.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3910..5268)
                     /gene="trpCF"
                     /locus_tag="RO454_003706"
     CDS             complement(3910..5268)
                     /gene="trpCF"
                     /locus_tag="RO454_003706"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDY0935472.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(5272..6867)
                     /gene="trpD"
                     /locus_tag="RO454_003707"
     CDS             complement(5272..6867)
                     /gene="trpD"
                     /locus_tag="RO454_003707"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDY0935473.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRCVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6867..8429)
                     /gene="trpE"
                     /locus_tag="RO454_003708"
     CDS             complement(6867..8429)
                     /gene="trpE"
                     /locus_tag="RO454_003708"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDY0935474.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8451..8544)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8521..8565)
                     /gene="trpL"
                     /locus_tag="RO454_003709"
     CDS             complement(8521..8565)
                     /gene="trpL"
                     /locus_tag="RO454_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDY0935475.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8703..9584
                     /gene="yciV"
                     /locus_tag="RO454_003710"
     CDS             8703..9584
                     /gene="yciV"
                     /locus_tag="RO454_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDY0935476.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9581..10201
                     /locus_tag="RO454_003711"
     CDS             9581..10201
                     /locus_tag="RO454_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDY0935477.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10229..12124
                     /locus_tag="RO454_003712"
     CDS             10229..12124
                     /locus_tag="RO454_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDY0935478.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGISEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            12337..>13206
                     /gene="rluB"
                     /locus_tag="RO454_003713"
     CDS             12337..>13206
                     /gene="rluB"
                     /locus_tag="RO454_003713"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDY0935479.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNN"
BASE COUNT         3171 a         3302 c         3481 g         3252 t
ORIGIN      
        1 acaaggggtt aaatgctgcc gcagcatagc gatattgaaa tagcctggta tgcttcgata
       61 cagcaggagc cgaatggctg gaagaccgtc accacacagt tctacatcca ggaattcagt
      121 gagtatattg cgccactgca ggatgctgta gatctggaaa tcgcaacgga ggaagaaaga
      181 tcgttgctgg aggcatggaa taaatatcgg gtattgttga atcgtgttga tacatcaact
      241 gcacctgata ttgagtggcc tgcaaatcct gtcagggagt aatcattggg attatgccgc
      301 agacacgtcg tatgcaggaa cgtgctgcgg ttagtttgtg agctttcgat agtggttgtt
      361 atttttgccc ttatttgttc cggaggccat ggttcaatgg tccgtctgcc ccctgtggtg
      421 atgtcagcaa aatcagccac tgcgcgaacc acaatagccc gggaagatgc tgaagatcac
      481 caggtaaagc tgtcagcgca gaaactggaa gaactgctcg catcaatggt taaggatgag
      541 gttgatcgca atgatgggat ttattgacgt cagcgagagc agaaggaaga actgaataac
      601 ctgaatgatt tacgctcaat cagagcgatg gcgattagcg gcaatcacgc tcgataaaat
      661 taaccccggt gatcatccgg ggttttgcat tcattaaaac cgcatcaacc tttccaccaa
      721 ttgttcttta cgggcaacga tccacccatg ttgctccaga taaaatttaa accgttccag
      781 cgtgcatacc atcgcatcgg cggggacttt ttctgtgaac tcgacctgac cgtgtttatc
      841 gaagtggatc agtaatgcgc atccattatt tggggcgggg gagttttgtg ctgcaggagg
      901 acgtttgtgg agttcttctt ccattgcgtt aaatttttca atgtaagctt cttttagtct
      961 ggctgctttt ttgccagtaa atcccattac gaggaacatg aagccgtctt ttgtgatcag
     1021 gtagcatcgt gattcacgtt ctgcgccgtt gccaatttcg acagtctgaa catcggccga
     1081 aaagttggct gatgtaaatt cgggagagca gtcgagtgtg tcaatcttag ctaaaacgtg
     1141 tttatgttgt ttatcgaagt agtcggcaac agcttgagag gtggtaacca ggcgaccatc
     1201 tttgaaaaag acttttggtg actcgttaga aaaagttata gctgtattca tatgtgatga
     1261 ctccaattaa ttggatgatc accacctctg acgccaatca gggaggtggt gagacgtaca
     1321 gggttggcgt aaccggtcat cacaaaaccc ggcgcggatt tctccgcccc tgcacgcccc
     1381 accataatgc gaatgtggca atgcttaacg caaaaaaaac cgcaaacgcg gctatgcgtt
     1441 gtgatgttat ccgagacgcc aatcccgaca accgattttg cggttgtgtg aggaatatag
     1501 ccccggagaa caaaaactgt caacgggcaa aaaaatttcc gttatcagga atgtataaaa
     1561 ttaaagcgat cgagcaaaaa atcctttctg caaaactaaa aaagatttca ataaaatcag
     1621 gcagttaaaa acacggaaaa acggtataaa aaagcgcctc aaatcctgtt ttttctttca
     1681 gatacaatgt cttattgcat tttttaaaac atgtcggtct ggtacatgga tatcgatacc
     1741 gaagtgaaat ataaatccaa cggcgcagtg ctgcctgcag ggcgttattc tgacaacgta
     1801 cgccttgacc cgtgggtatt tatgttctct gcaggttacc gcttctaatt ttttaccatc
     1861 aataaaccgc cagatgtttt ctggcggttt gtctcaaatt aacgtcgtgt agtggctttc
     1921 atcggttgta caaaagcttt cagtgccgcc agcattttct ctggctcatt aatatgttgc
     1981 tcgatgattt taacgatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct
     2041 gcttttacct gatcaggggc ggaaatacca aatccctgca atggaggtgc agcgttgtac
     2101 tctttcagct tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc
     2161 acgcccgcac gtgacaataa ataggtgtat ccacgaccgt aggaggctat ctggcgcagc
     2221 aaatcatcgt cggcattcgg cgggcaaata aagataggtg cgacattatg acgcaacgcg
     2281 gcctggcgga agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg
     2341 ccgactttct cgcactgggc gtaaaactca tcaatgcctt tgttaaacac caggttggcg
     2401 tacatcagca ggccaacagg aatggtcgga tgtttttgac gaatcagtgc cagcatctca
     2461 aagcactgcg ccggggtcac acccgccgca aaggcacgca gtgtggcgtt ttgaatcgtc
     2521 gggccatccg ccagtgggtc ggagaagggg atgcctaact ccagcgcgtc agcaccggct
     2581 tcaattagcg tatcgataat tttcagcgac tgctcaatgc ccggatcacc gagggtgacg
     2641 aaaggaacga atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt
     2701 tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc
     2761 cgcgaccgga aaggttaacc accagtagct gctctttttc cggattttcg cgcatcattt
     2821 tcagcgcatg ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcatgcaggc
     2881 aaagcgtttt aaaggcttcc agggcttcat cgtcggtaat agacacgtaa tcagcgcgtc
     2941 cagtgctgtt gagatacgca tgttgcgggc cgacggacgg gaaatccagc ccggcagaaa
     3001 tggagtaaga ctcttcaatt tgcccgtctt cggtttgcat catcggcgct ttcataccga
     3061 aatagatgcc cacgcgacca tgttttaacg gtgcgccgtg ctcgccagtt tcgataccgt
     3121 gaccgccagg ctccacacca atcaggccga cgtcggtttc gttgatgaaa tctgcaaaca
     3181 taccgatggc attcgaacca ccgccaacac aggcgataac ggcatccggc aggcgacctt
     3241 ctctttccag aatctgcgct ttcgtttctt cgccaatcat ccgctgaaac tcacgcacaa
     3301 tggtcgggta aggatgcggg ccagctgcgg tacccagcat atagtgcgcg gtttcataac
     3361 tgccggacca gtcgcgtagc gcctcattac aggcatcttt cagggtcgcg gaaccgctat
     3421 gcaccgggat cacttccgca cccattaagc gcatccggaa aacgttaggc gactggcgtt
     3481 caacgtcttt ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg
     3541 ccgacgccac gccatgctga ccggcaccgg tttcggcaat aatttcagtt ttacccatcc
     3601 gcttcgccag taaagcctga ccgagtacct ggttagtttt atgcgcgccg ccgtgcagca
     3661 aatcttcgcg cttcagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg
     3721 tcagcgcggt tggacgcccg gcatagtttt tcagcaggtc gttgaactga gcctgaaatt
     3781 caggatcttt ttgcgcgctg acaaaagctt cttccagctg gcgcagagcg ggcatcagga
     3841 tttgtggcac gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg
     3901 ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt
     3961 gatacccggt tgcgactcta cagcagaatt aaaatcaagc ccggcgcagc cggtttgtgc
     4021 cgcttccacg cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg
     4081 accatttaat agtgaccagt cgaaacgttg tccgctcccg ccctgaccgt tgtcgaatac
     4141 atatttatcg atgtgctgaa aatcgcgcgc gggaagagtt tcaccgacac ttaaagcctt
     4201 ccagatggcg acgtgtgctg gcagagcctc acgcagagtg tcgatataca gctgatcttc
     4261 attaccatgc agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc
     4321 aatatcgtga ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg
     4381 cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat
     4441 cgcgcccgcg tcataagctg ctttagcatc ttgcccacgt gtcaggccac atactttatt
     4501 ctcacccagc aacacccgac gcacagcggc gttcaaatcg tcatgggcca tcaacgccga
     4561 accaatcaga aagccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat
     4621 gccggattcg ctgattaccg tcacgttgtg ccccagtttc ggtgcaagct cgcgggtacg
     4681 gttgagatca atcgacaaat cgcgcagatc gcggttgttg atgccaacga cctttgcccc
     4741 caatgcaatg gcgcgctcca gttcctcttc attactgact tcggtcagca cacccatctc
     4801 cagactgtgg gcgacggctg caagctggcg atattgttcg tcatccagta ctgaaagcat
     4861 taataagcag gcatcggcct ggtaatagcg cgccagatag atctggtaag gatcgataat
     4921 gaagtcttta cataaaatcg gctgcggggc gatttggctg acgatgggga ggaaatcaaa
     4981 gctcccctga aaatatttct catcagtcag cactgaaatt gccgaagcgt aatgtttata
     5041 aatggcagca atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc
     5101 ttttttgcac tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg
     5161 cgtgctcggc tgaatctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggcttc
     5221 tacccaaatc gccttgtctg cgacgatttt cgctaaaacg gtttgcatta tttaccctcg
     5281 tgccgccagt gcggtgactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt
     5341 ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacattcgc
     5401 cgcgacggct gcttcgtgag cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc
     5461 acggttttct tccggtgttc cgcctgccag ttgctcctgg tggtagggtg tcaggccaaa
     5521 atcttcagcg gtcaattgat agctcttaat ttcgccgtca tgcagttcgg caacgattgt
     5581 cggcgcgtgt aatgaaactt catccatccc gccgctgtgc accactgccg cgcgttgata
     5641 ccccagcacg cgcaaggttt cggcaatcgg cagcaccagc tccgggctgt agacaccaat
     5701 taacgccagc ggaggatgcg ccgggttaat taatggcccc agcacattga acagtgtgcg
     5761 ggttttcagt tgctggcgaa ccggcattgc atggcggaat ccggtgtgat atttcggtgc
     5821 aaagaggaaa cagacgccta attcatccag cgcctggcgc gatttatcgg cgttcatatc
     5881 aagattaata ccgaacgccg ccagcagatc cgacgagccg gatttactgg agacgcaacg
     5941 gttgccgtgt ttcgccactt tcagcccgca ggccgcggcg acaaacgcac tggcggtaga
     6001 aatattgatg ctgttgctgc cgtcaccgcc agtaccgacg atatcggcaa acagataatc
     6061 cgggcgcggg aacggcgcgg cgttttccag tagcgcggtt gctgccccgg cgatctcgtt
     6121 cgggtgctca ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag
     6181 ctcgccacgt accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgtgc
     6241 ctgatacagt ttttccagaa tcggttgcag cgtgttggtt tgctctagtt tctgctgcgc
     6301 ccaggccagc gtttgttcca gcaggcgagc gccctgggta gtaagaatgg attccggatg
     6361 gaactggaat ccacaaacgc gatccgcatc gtggcgcacc gccatcacca tgccattaaa
     6421 atgggcgttg atggttaaac cggccggaat attactgcca accagcgagt gataacgcgc
     6481 cactggcagc gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga
     6541 cgctttaccg tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat
     6601 cgcctgatgt ccgaggcaaa tgccaataat tggcagcttg ccacgcaagc gggtgaggag
     6661 ttccggcata caaccggctt cgctcggcac accggggcca ggagaaagca tcagcaccgg
     6721 attgctcatc gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat
     6781 caccacgtta tgaccattgc tgcgcaactg atctgccagg ttgtaagtaa aagagtcgat
     6841 attatcgagc agcagaatgt cagccattag aacgtctcct gtgcatgatg cgcggtggca
     6901 atagcgcgca gtacagcgcg ggctttatta cgggtttcgt cggcttccga ctgcggaaca
     6961 gaatcaagta ccacgccagc accggcttgc acggtggcga ctccgttctc taccagcgcc
     7021 gagcggatca caatgcaggt gtcgagatcg ccatgcgcgg taaaataacc taccgcgccg
     7081 ccgtagctgc cgcggcgacg accttctgct tcggcaatta actgcatagc gcgtactttc
     7141 ggcgcaccgc ttaacgtccc catattcata caggcgcggt aagcgtgcag ggcgtcgaga
     7201 tcgtggcgca gctcaccaac aacgcgggag accaggtgca tcacgtaaga gtaacggtca
     7261 actttggtga gatcggcgac gtagcggctg ccgggggtgc aaatacgcgc caggtcatta
     7321 cgggcgagat ccaccagcat cagatgttca gaaagctctt tatgatcggt acgcatctcc
     7381 agttcgatgc ggctgtcgag gtctctatcc agtgaaccat cggcacgacg accgcgtgga
     7441 cgtgttccgg caatcgggta aatctcaatc tggcggctgg tggcgtcata cttgagcgaa
     7501 ctttccggcg acgcgccaaa cagggtgaaa tcattatcct gcataaaaaa catgtacggg
     7561 ctgggattac tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag
     7621 aaacggcgag atggcaccac ctggaaaatt tctccggcgc gaatcgcttt ttgcaacaaa
     7681 cgcactacgc caccgaactc ttcatcgctc tggttacatt cacaacgcat atgcggcacg
     7741 gaaaccaccg gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttaaggcga
     7801 gcagtgagac gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg
     7861 ctttttttct gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg
     7921 cagttatttt ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag
     7981 aacaggccgc cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgt
     8041 aataagcgga atgcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt
     8101 ggactgacag gcgggaagcg cagtacgcgg cagtttggtg attgttcatt ttccacaccc
     8161 gcaggcaggg cgttatccag tagtgccagc agggcttcgc cattgccgga aagtgcctgg
     8221 attgtgacgg tgtcacctaa tgctgtaatg cgcagcgcac tgtctaccag tagcaggctt
     8281 tttaaatcat ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgatcc
     8341 ccacacaact ggtgaaaaag cgcggttggg ttgtcgcgat aagcgcctac gcaggttagc
     8401 agttcgagag tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc
     8461 ccgctcatta ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt
     8521 tcaggaagtg cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt
     8581 tacgtgaact tgcgtactag ttaactagtt cgatgattaa ttgtcaacag ctcatttcag
     8641 aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg
     8701 ccttgagcga cacgaattac tcagtgattt acgacctgca cagccatacc acagcttccg
     8761 atggctgcct gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc
     8821 tggcgatcac cgaccatgac accacagctg caattgcacc agcaagagag gaaatttcac
     8881 gttcgggact ggcgctgaat cttattcctg gtgtggaaat ttccacggtc tgggaaaatc
     8941 atgaaattca tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc
     9001 tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct gattgccgaa agacttgaaa
     9061 aagcacaaat acctggggca ctggaaggcg cgcaacaact ggcgcagggc ggtgcggtga
     9121 cgcgcggtca ttttgcgcgt ttccttgtgg agtgcggaaa agccagttca atggcggatg
     9181 tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa
     9241 tagaacaagc tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatccag
     9301 ggcggtacaa tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc
     9361 acggcgacgc gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg
     9421 cgacacttgc gcgtcagcat catttatggg catcacaagg atctgatttt catcaaccat
     9481 gcccgtggat cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc
     9541 agctatggga acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat
     9601 tcatcctgat aacccgcagc aacgcctgat caaccaggcg gtggagatcg tgcgtaaagg
     9661 cggggtgatt gtttatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa
     9721 aaacgcgatg gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct
     9781 gatgtgtcgc gatctttctg aactctcgac ctattcattt gtcgataacg ttgcgtttcg
     9841 tttgatgaaa aacaacacgc caggcaacta taccttcatc ctgaagggga ctaaggaggt
     9901 gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt atgcgcgtgc cgtctaaccc
     9961 gattgcgcag gcgctgcttg aagcattggg cgaaccgatg ctttccactt cgctaatgct
    10021 gccaggcagt gaatttaccg aatccgatcc ggaagaaatt aaagaccgtc tggaaaaaca
    10081 ggtggatttg attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct
    10141 caccgatgat acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata
    10201 acaaattgcg ttccacggat ggaagattat ggcagggata tatcgctgca ttttgctatt
    10261 gatagttggg ctttttttct cttcgttgag ttatgcgaaa aacacggaga tcccttctta
    10321 tgaagaaggg atctcgctct ttgatgttga agccactctg caaccgaatg gggtgctcga
    10381 catcaaagag aacattcatt ttcaggcgcg aaatcagcag attaagcacg gattttatcg
    10441 tgatttaccg cgactctgga tgcagcctga tggggacgct gcactgctga actatcatat
    10501 tgttggcgtc acccgtgatg gtatttctga accctggcat cttgactggc atatcgggtt
    10561 aatgagtatt gtcgtgggcg ataaacaacg tttcttgcct caaggcgact atcattatca
    10621 aattcattat caggttaaaa atgctttcct gcgtgagggg gattctgatc tgctaatctg
    10681 gaacgtgacc ggtaaccact ggccgtttga aatatataag accctatttt cactcaagtt
    10741 gcctgatatt gcgggtaatc catttagcga aatcgatctc tttaccggag atgagggcga
    10801 cacatatcga aatggccgca tccttgagga cggaagaatt gaatctcgcg atccgtttta
    10861 tcgtgaagat ttcacggtcc tctaccgctg gcctcacgct ttacttggta atgccccggc
    10921 accacaaacg acgaatattt tcagccatct tcttttaccc tctacgtcat cgttgttaat
    10981 ttggtttccg tgcctgttcc tggcgtgtgg atggttatat ctctggaagc gcaggccgca
    11041 atttacgccg gtagatgtga ttgaaaccga tgtcattccg ccagattaca cgcccggcat
    11101 gttacgtctc gatgcgaagc tggtttatga cgataaaggt ttttgcgccg atatcgtaaa
    11161 tctgattgtg aaaggaaaaa ttcatctgga agctcattat gacaagaacc agcaaatcct
    11221 gattcgtgtt aatgaaggcg cgaccagaaa taatgcggta ttactgcccg cagagcagtt
    11281 attactggaa gcgttatttc gtaaaggcga taaggtcgtt cttacgggga gacgcaacag
    11341 agtcttacgc aaggcatttt tacggatgca gaaattttat ctgccgcgta aaaagtcttc
    11401 gttttaccga cctgatgcgt ttttgcaatg gggcgggatg gcgatattgg cggtcattct
    11461 ctacggtaac ctgagtcccg taggttgggc aggaatgagt ctggtaggcg atatgtttat
    11521 tatgatctgc tggcttcttc ctttcttatt ttgttccctt gagcttttgt ttgcccgcga
    11581 tgatgacaag ccttgcgtta atcgtgtaat catcactttg tttttaccac tgatttgttc
    11641 aggcgtggcc ttctattctc tctacatcaa tgtcggagat gtattctttt actggtatat
    11701 gccagcgggt tattttagcg ctgttttcct gaccggttat ctcactggca tggggtatat
    11761 ttttctgcca aagtttaccc aaactgggca gcaacgttat gcccacggtg aagctatcgt
    11821 taactatctt gcgcgtaaag aggcagcaac acacagtggg cggcggagga aaggggaagc
    11881 acggaaactg gattacgctt tgttaggttg ggcggtctcg gcaaaccttg gcagagaatg
    11941 ggctgcacgt atcaccccat cactcacagc ggctgtccgc gccccggaaa ttgcccgtag
    12001 tggcgttttg ttctcattac agatgcatct gagtctgggg gccaatacca gtttgttggg
    12061 gcgaagttat tccggtggtg gtgctggcgg cggtgcgggt ggcggaggcg gtggtggctg
    12121 gtaataaagc tgatgttaat cgaaacaagc tttaaccata aagatagtgt agtatgttgc
    12181 gcctcaaagc caggccagtt aaaacgtcga gtcgtttact taaggcctga agagttcaaa
    12241 cagactttac tatcagtgag tcagatgtaa gttctgactc attgttgcac aaaagcatat
    12301 cagacgcctg ggaaggcgac accaaaagga agctctatga gcgaaaagct acagaaagtg
    12361 ctggcgcgtg ccggccacgg ttctcgccgt gaaatcgaat ctattattga agccggtcgt
    12421 gtgagtgttg atggcaaaat tgctaaactc ggcgatcgcg ttgaaattac ccctggactg
    12481 aaaatccgta tcgatggtca cctgatttcg gtacgtgagt ccgctgaaca aatttgtcgc
    12541 gttctggcct attacaaacc ggaaggtgag ttgtgtaccc gtaacgatcc ggaagggcgt
    12601 ccaacggtgt ttgaccgtct gccaaagctg cgtggcgcac gctggattgc cgtaggtcgt
    12661 ctggacgtta acacctgtgg tctgttgttg ttcaccaccg acggtgaact ggcaaaccgt
    12721 ttaatgcatc caagccgtga agttgaacgt gaatatgccg tgcgtgtatt tggtcaggtt
    12781 gacgacgcga aactgcgtga tttgagtcgt ggcgtgcagc tggaagatgg tccggcagcc
    12841 tttaaaacca tcaagttcag cggcggcgaa gggatcaacc agtggtacaa cgttactctg
    12901 accgaaggcc gtaaccgtga agttcgtcgt ctgtgggaag cggttggtgt gcaggtgagc
    12961 cgcctgattc gcgttcgtta cggtgatatc ccactgccaa aaggtctgcc acgcggtggc
    13021 tggacggagc tggacctcgc ccagactaac tatctgcgcg aactggtgga actaccgcca
    13081 gaaaccagct ctaaagtcgc tgtagaaaaa gaccgtcgtc gtatgaaggc gaatcagatt
    13141 cgtcgtgcgg tgaaacgtca cagtcaggtg agcggcggtc gtcgttctgg cggacgtaat
    13201 aataac
//
LOCUS       DAPQZD010000135        13203 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPQZD010000135 DAPQZD010000000
VERSION     DAPQZD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.137"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            69..1799
                     /locus_tag="RO454_003714"
     CDS             69..1799
                     /locus_tag="RO454_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDY0935480.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            1796..2710
                     /locus_tag="RO454_003715"
     CDS             1796..2710
                     /locus_tag="RO454_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDY0935481.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2742..2990
                     /locus_tag="RO454_003716"
     CDS             2742..2990
                     /locus_tag="RO454_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDY0935482.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2990..4162
                     /locus_tag="RO454_003717"
     CDS             2990..4162
                     /locus_tag="RO454_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDY0935483.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4197..5276)
                     /gene="lptG"
                     /locus_tag="RO454_003718"
     CDS             complement(4197..5276)
                     /gene="lptG"
                     /locus_tag="RO454_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDY0935484.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5273..6343)
                     /gene="lptF"
                     /locus_tag="RO454_003719"
     CDS             complement(5273..6343)
                     /gene="lptF"
                     /locus_tag="RO454_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDY0935485.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6374..6934)
                     /locus_tag="RO454_003720"
     CDS             complement(6374..6934)
                     /locus_tag="RO454_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDY0935486.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6946..7770)
                     /locus_tag="RO454_003721"
     CDS             complement(6946..7770)
                     /locus_tag="RO454_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935487.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(7770..9119)
                     /locus_tag="RO454_003722"
     CDS             complement(7770..9119)
                     /locus_tag="RO454_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDY0935488.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9165..9938)
                     /locus_tag="RO454_003723"
     CDS             complement(9165..9938)
                     /locus_tag="RO454_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935489.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSPA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9970..10683)
                     /locus_tag="RO454_003724"
     CDS             complement(9970..10683)
                     /locus_tag="RO454_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDY0935490.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
     gene            10857..11549
                     /locus_tag="RO454_003725"
     CDS             10857..11549
                     /locus_tag="RO454_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDY0935491.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRSVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEK
                     AITDFMTARGYH"
     gene            complement(11598..13097)
                     /gene="pitB"
                     /locus_tag="RO454_003726"
     CDS             complement(11598..13097)
                     /gene="pitB"
                     /locus_tag="RO454_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDY0935492.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         3341 a         3420 c         3252 g         3190 t
ORIGIN      
        1 catttatctt ttatgcctat ataatccacc gcattattgt cattttattt ttgatatcca
       61 attttttcgt ggtgtatatg tctaataaaa tctttacgca ttccctacct atgcgctatg
      121 ccgattttcc aacgctggtt gatgctttgg actatgccgc tctgagtagc gctggaatga
      181 atttttatga cagacgttgc caacttgaag atcaactgga atatcagacg ttaaaagcgc
      241 gtgccgaagc tggtgcgaag cggttgttat cgctgaacct gaaaaaaggc gatcgcgtgg
      301 cactgattgc cgaaacgagt agcgggtttg tagaggcttt ttttgcctgc cagtatgccg
      361 gcttagtcgc cgtcccgttg gcgattccaa tgggcgtcgg tcagcgggat tcctggagcg
      421 ccaaactgca ggggttactg gcaagttgcc agcccgcagc cattatcact ggtgatgagt
      481 ggttgccact ggtcaatgcc gcgacgcata acaatcccga attacatgtt ttaagccacg
      541 cctggtttaa ggcattaccg gaagccgatg ttgcgcttca acgtccagtt ccaaacgata
      601 tcgcctacct ccagtacacc tccggcagca cccgttttcc ccgtggcgtt atcattactc
      661 accgcgaagt gatggctaat ctacgtgcta taagccacga tgggattaaa ttacgccctg
      721 gcgaccgctg cgtctcctgg ctgcctttct accatgatat gggactggtc ggctttctcc
      781 tgacccccgt cgccacgcag ctttcagtag attatttgcg cactcaggat tttgccatgc
      841 gtcctctgca atggcttaaa ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc
      901 cgtttggcta tgaattgtgc cagcgccgcg tgaatgaaaa agatctcgct gaactggatc
      961 tttcctgctg gcgcgtcgct ggtattggtg cagaacccat ctccgcagaa caactccatc
     1021 aattcgctga atgtttccgt caggtcaact ttgacgataa aactttcatg ccgtgctacg
     1081 gactggcaga aaatgcgctg gctgtcagct tctctgatga agcttccggg gttgtggtta
     1141 acgaagtgga tcgcgacatc ctcgaatacc agggtaaagc cgtcgcgccg ggtgctgaaa
     1201 cacgcgccgt atcgactttc gtcaactgcg gtaaagcgtt gccggaacat ggcattgaaa
     1261 tccgcaatga agcaggtata cctgtcgcgg aacgtgtggt aggccatatt tgcatctccg
     1321 gccctagcct gatgagcggt tactttggcg accagatttc acaagacgag attgccgcga
     1381 cgggctggtt agacaccggc gacctcggtt atctgctgga cggttatctg tatgtcaccg
     1441 gacgcattaa agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat
     1501 atattgcgga acaggaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgccc
     1561 aggaaaaaat cattttgcag atccagtgtc ggatcagcga tgaagaacgt cgcgggcagc
     1621 ttatccacgc gctggcagca cggatccaaa gcgaatttgg cgttaccgcg gatatcgatc
     1681 tgttgccgcc ccacagtatt ccccgaacat cctccggcaa gcctgcccgt gcggaagcga
     1741 aaaaacgtta tcagaaggct tatgctgcca gtcttcatgt gcaggagtcc ctggcatgaa
     1801 tcaaactgtc gcggtgacgg gcgctaccgg gtttatcggt aaatatatta ttgataacct
     1861 gctcgcccgc ggctttcacg ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga
     1921 taatctcact tgggtgcgcg gttcgctgga agatacacat tcgcttagcg aactggttac
     1981 cggagccagc gcggtggtcc attgcgccgg gcaagtgcgc gggcacaaag aagagatttt
     2041 tacccgctgc aacgttgacg gcagcctgcg cctgatgcaa gcggcaaaag agagcggctt
     2101 ttgccaacga tttctgttta tctcttcgct ggcggcgcgc catcccgaac tctcctggta
     2161 cgcaaattcc aaacacgtcg ccgaacaaca gctgactgca atggctgacg caattacgtt
     2221 gggcgttttt cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt
     2281 tgactggatg ctgcgcggtc tgctgccacg acttggcacg ccagagacac agctctcttt
     2341 cctgcacgtc accgattttg cgcaagcagt gggtcagtgg ttaagcgccg aaactgtaca
     2401 gacgcaaacc tatgaattat gcgatggcgt cgccggaggc tatgactggc aacgtgtcca
     2461 acagcttgtt gccgatgtcc gctgtggttc cgtgcggatg gtcggtattc ctctgccgct
     2521 actcacctgc cttgcggata tcagtaccgc gttgagccgt ctggcgggta aagaaccgat
     2581 gctgactcgt tcgaaaatcc gtgaattaac ccatgccgac tggtcggcaa gtaataaccg
     2641 tatttctgaa gatattaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg
     2701 gctattttga ttgtcttgtt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag
     2761 taatggatta catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc
     2821 ccgacagcga tctcgtcaac gatcttggcc tggagtcgat caaagtgatg gatctcttaa
     2881 tgatgctgga agacagattc gatatttcca ttcctattaa tattctgctg gatgtcaaaa
     2941 cccctgcgca attaatggaa actctacttc catggctgga gaataaataa tggggctata
     3001 cgataaatat gcgcgccttg ctggcgagcg actgcaattt agtgacaacg ggttaacacc
     3061 gtttggcacc tgtattgatg aagtgtattc cgccaccgaa ggtcggatcg gcaataaaaa
     3121 agtgattctg gccgggacca ataattatct cggtttaacc tttaatcatg atgccatcgc
     3181 cgaaggtcag gcagcgttag cggcccaagg caccggcacc accggttctc gtatggcaaa
     3241 cggcagttat gcgccgcacc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc
     3301 taccgctatc gttttttcaa ccggttatac cgctaattta ggcgtcatca gcgcacttgc
     3361 cgaccataac gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg
     3421 ttctctcggt ggcgcagaaa ttattcgttt ccgccataac gatgctaaag atctggaacg
     3481 gcgcatggtt cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg agggcatcta
     3541 cagcatgctc ggtgacgttg cgccgttagc ggaaattgtt gatatcaaac gtcgtctcgg
     3601 tggttatttg attgtcgatg aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg
     3661 actggctgaa gccgttggcg ttgaagatga tgtggatatt atcgttggta ctttcagtaa
     3721 aagtctggcc tcgattggcg gttttgctgt cggttcggaa gcgatggaag tgctgcgcta
     3781 cggcagccgc ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg
     3841 ctcgtcgtta agaaccattg ccacacaacc agagctgcgg caaaaactga tggataacgc
     3901 caaccatctg tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc
     3961 ggtagtgcca gtaataatcg gttcgaaaga ggaaggttta cgcatctggc gcgagcttat
     4021 ttcgctgggc gtttatgtga atcttatcct gccacctgcc gcgcctgcag gcatcactct
     4081 gctgcgctgt agcgtaaacg ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc
     4141 atttgctact ctgaagcagt aatgtgagcc cgctgtggtg ttgcgccacg gcgggtttaa
     4201 tgttgtctgt cataccaata caccaggctc aacgccacca acacaaacgc taccggtaaa
     4261 ccgagcgcgg taacaggtgc gctcaacgca aataacaatc ccagattgac catgatttga
     4321 tagctgatcc aggtgagtaa gccaacgatg acgcctacag cgagacggct acccatccct
     4381 ggcgagcgcg gggcgctaaa ggtgaacggc acagccagca aaatcatcgc cagggtcagg
     4441 atcggttgcc ccagtttttc ccacagtgca aggcggtatt cgctgctggg ttgtccggtt
     4501 tcctgcaaat aatggatgta atgattaagc tgacggacag aaaaagtatt ccccggcatc
     4561 gataactctt caagatccat actggtgaag gccgattgcc aggcaagatt atctgatgtt
     4621 tccagcgttt ctttgccatt caaccatttt ttatgattca caccatgcag gatccacgtt
     4681 ttgtcgtctt caatggaggc actgcgtgca aaaatgtagg attcgagcga gagatcgtcg
     4741 cgataatgaa atatctccac gcccacaggc tggccctgct cgttcagcga tttcaccgtc
     4801 acaaattcat taccgcgcct ggcccaaagc atattgccgg taatgtcatc gtcctccccc
     4861 aacgcggtgg cagtcgattt gatttgcagc gcctgttgct gcaatggcga cgccacccac
     4921 tcatctatcg cgcctaacga aacagtccac aatatcccgg caaccagcgc caccagtgca
     4981 atacggaaga tagaaaaccc agtgctgcga atggcggtaa gctcactgtt tttcgataac
     5041 tgccccaggc caacaatccc tcccaataat gcgataaacg gcgaaagttc gaccagtgta
     5101 cgcggtaagg tcatcagcac caccagcacc gcctgagtcc agcgataacc gcccgggctg
     5161 acatcatcca gttcgttaat caggttgaag gtggtaaaaa gcggcagcaa tagccctgcg
     5221 gcggcggcaa aaccgagaaa gagatgacgg attaaatagc gactgaaaac attcatcggg
     5281 aaaatttctg cagcaaagag aagtcgcgtg cgaccagcat cagcaggccc attagcatga
     5341 gtccgggtac taaccacaaa ccaggaatga ggggaatcgc accgttagcc accagcgtac
     5401 ggcagacgtc gccgccataa aaaatggcaa caaacagcaa tgtcagtggc aataacgttg
     5461 aaaatcgccc ttgccgcggt tttacccgac ttaatgaaat ggccagcagc gccatcaaca
     5521 atgctgtcag gccacggctt tgtcgccatt gcagttcggc atgatcggca ggaaaggctg
     5581 agcgcgccag ctccgtgacc gattttgctt tgcgtttgac gttagggctt tgatccagcg
     5641 gattcagatg taattgcaga ttacggtaga tctgttcgtt gtcgtcacgc ccctgatgat
     5701 cgagaagata ggcggtcccg ttatgcaaca taacggtcgg tttctcaggt gatgggtcaa
     5761 ccacatcaac cgaacgggcg cggaaaatgc gggttcgatt ggcagtagaa gtataaatca
     5821 gcgcgtcagt caggcgatta ttatcctgat caaccgtctg cgaaaggatc attcgtccgt
     5881 tatcgttagt gttaaatttc tttgcccgca actggcgaac atccagctcc gactgcgact
     5941 gttgctccag ttgataaatc tgcgcataag cccacggccg accatacatc gacagaaggg
     6001 tgacaaaaat acttagcggt atcgccagat aaagaacggc tttgtacaag cgtcccggat
     6061 tgccccccgc cgcagagata gcggtaattt ccgagtcggt atacatctgc cctagcgtca
     6121 cgccaactga cacatacaga ccaacaggta acaacatctc cagtgcaatc agcactttgt
     6181 agaaaacgat atccagcaca acatcaagag ctaaggtccc atttgccgct tcggtcagat
     6241 aacgctgtgc ggagtagctg gcgaaaataa agatcagaaa accgacgata atcagcacca
     6301 gacggcgcgt accacgcatg atgtagtgtt caaccagctt catctcacta acaccctcaa
     6361 tcctttgaat tttttattca ttgatcagtt tctgcgccag cgcaatgaca tgatctacct
     6421 cttccggcgt caggctgccg tcctttgccc actgaatttg cccgtcttta ttcagcacca
     6481 gaatggttga actctgctcc ggcaaccgcc aggcaacgcg tcccagtcca ttgccgtcaa
     6541 taacgaattg cgcccacgga tagcggcgtt tatttttttc gattttgcca acgacaaaat
     6601 agccagtacc aaagatggca tcatcggtgt tcacaatggt ggtgggctga aaacgatcct
     6661 gcggaaagtt tgccgcttca accgctttaa ttaacagtga gttctttttc ttcgccgact
     6721 tacgcccggc gatatactgg ataatgcgga cttttcccgc cagctgtgtg ctgttccact
     6781 tttggtagtg aatttcgtcg tcgctattca gaagcagctc gccgccttcc tggatataaa
     6841 cgggggtaac cgttttgcca gtgacaaaat tgtgtgcctg cacaccctgg gctaagagca
     6901 tgataatcag cgcaataatc aaacgagatg acatcattgc tccggttata gcggaaaacg
     6961 cgaaatcagc agctctaagc gctggcgaat atcgctatcc agcgccaccg gtgcatcata
     7021 cgactggcgg ttttccatat catccgccac catgtcgtcg tgtttatccc ccatcaggcc
     7081 attaatggcg gcgaggaagc gggaaatctg taacgcctct ttattttcag gatcaagcga
     7141 tcttaaggtg tcgtaataag aaacctgctg gctgatgatt tttcgcacat catgaaattc
     7201 cctgccggtg agtcgcggtt tggcaattat ctctttcagg gtatgcattt gcttttgccg
     7261 ataggcaata aaaccgctta cgctttctaa ctggctatgg cgtaatgaat agttcaccaa
     7321 cgaccagacc ggagagctta aataaacccg taacaagttg ccgtaatacg acacaatatt
     7381 tttcttaccg ttacgaaaac cgtcctgaaa acgccccaaa aaaacggtcg ttttgccgaa
     7441 taaaaatccg gcctgatgct tctttaaata cagccgttgc gcaaaacgca ggtgcttgta
     7501 gcgcgctcgc atgtatttaa attgtttgcg ctcatcagca gtcagctcat cgcctttggc
     7561 ctgtttgcat attaaacgca gtaactcctg gcgattgacg ccattgagcc agaattgcag
     7621 gcataacgcg taattatcat ggatgatttc ggtggtacaa cttagtgcga tagtcggcgg
     7681 caaactgacc tgtgcatcaa cgacatcatc ttcttcaact gcaacaaata aacgctcgct
     7741 cgcctcaagc gggaaatatt ttctcatcat caggatttct ctccggctgc gttataaagc
     7801 aaggaatggc gctgaaaaaa tgcctttaag gtcggaatta acgagagcac cacatccaga
     7861 cattgcccac cgaaataagc aaacgctgct cccagcacgc caaacagatg acagaacatg
     7921 aacagcaata cgatgtagcc gattgaggcg atggtttgcg ccacgagaaa agcgcgctgt
     7981 tttcccgcca tcaatagcaa tgactcctgt gggaagccca gcatcgaaat cacaattgcg
     8041 cccagcatca cctgaatcag gtcatacgct tcgagatact taacgccaaa caccagcgaa
     8101 ataagcggct tgccgacgat caacaccgcg agcgccacca gaataccaat tccgcccgcc
     8161 agtaaacctg acttcacgcc taacaaccac ggtctggtgg tgcgcggatc taaacgcatc
     8221 acctctgggt aaaaactttt gcccagtaaa cccgctggcg ttccggcggc gtcgaagaat
     8281 gtcatggcga ttttaaataa cccggcggca gcgggtccta acacgatccc caccagcact
     8341 gtgctgcacg agttacgcgc cgaccagatg gagtgggcaa tgttggttga ccagacaaaa
     8401 ctccacgcgc ctttaatgtg tcgggcagac tcaaacagat tcaatttgaa ggcgttatgg
     8461 atatttctgc ggcgtaattc gcgcgcggca aaccaccagt acatggtgcc gccaaccaga
     8521 ttcgacacgt accaggcaat aacaaaaccc gcaaaaccaa agtcaaaata ccaggctacg
     8581 acgctccccg ctgcgcgcag aaaaggtttc gtcgcttgct gtacagcaat tagatcgaag
     8641 cgatctaccg cacgcagaat gccggtcggc gtggaggaag ccattgaagg aatgagcgtg
     8701 caatacagcg ctgccagcca aaagctttgg tcatccagac ctaatgaatg ggaaagaaaa
     8761 gggagtaagg caatgccgcc gacaatcgcc accgcgccgc tgacgatatc cagcgagaag
     8821 gaaaatgaga cgacattgcg gaattgctgc ggattattgt tggttaatgc tggtgttccg
     8881 tactgaacca ccagctgcca tatctgaaac ttaataaaat cgctgatcga cttggcgtac
     8941 gattgtacaa tcaccagtac gccaaacatg gcgggcgtca tccctttacc ggcacacgag
     9001 agcgccaaca gacccagtaa agcgctcacg acattactgg agcctaacca ggcgctattg
     9061 cgaataatgg tgcgaaacgc gccatctgca aaccaatgtt tgatgttaaa acccgccaat
     9121 tcagcctgac cttatcgttg atagtaaaaa gtatcccgcc agccttaagt taaacttcgg
     9181 cggtcagaaa cgatggcaac cagagaaacc gccttctgtg cctgttccag cacttcgctg
     9241 tagggcgctc tggaatcaat ctcaagaatt tttgtgccgt tatagccaat cgtcgacatg
     9301 acgccgattt tgtcctgcag ctcggcatag tcatggtcag gcttgcggga gatggcagta
     9361 tcaatatcaa tccccaaacg aatgattaat tcaggacggt attgcgccat tttttggtat
     9421 aaacgccgtt cgcgctgcgc cagaaacatg ctgattttcc cggtcgcacg ttcgacgccg
     9481 atccccggtc cgtcataata aaagcccgaa atttcagcct gcgggaagcg atcgctgacc
     9541 accagaacgc cactttgcgc cagtcgctga accttgcgta gattcgccat tcttcgcagc
     9601 gagaagcagt acataatcac cgccgcccat agcgctggag atttggtttt catgctttgg
     9661 gttttcatgc tttgggtttt cgatgatttg gccgccagtc gcctttcgag ccagacgcca
     9721 accaacggca atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     9781 cgctcggttt gccagtgttg ttgcagcgat ttcacgaggt cggtggtcag tgtggattta
     9841 ccggtgccat cacacccgac caccgcaatc agccccggaa tgtagttggg ctgcggcgcg
     9901 gttgtactat taacagtttg tgtttgtagt gcgtccattc gttaaatcat cccctatcca
     9961 ttttggtgat caggaaagcg cagcttgaat tgcgcgtagt gacgcttgca gaatttcatc
    10021 ggctggttgc cgaccatcca gttcaaggat cctggcgcca ttaaatgtca gttgcggcgt
    10081 aacggcgatt ttttcctgta acgccgccag ttggtggtca ggtttacgcg caaacgcggt
    10141 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
    10201 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
    10261 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
    10321 gagcagatag ccttgctggc ttttacacaa cattttgcga aacttatacg cccgccagca
    10381 ggaaagcaga tagataacca gcgcagtcat attgccaggc ggtgtggagg gcttttcgtg
    10441 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
    10501 tgaaatccat tcgccaattc gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
    10561 tgttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
    10621 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
    10681 cacgaaaaac ccggcagcaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
    10741 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
    10801 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
    10861 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
    10921 tgtgtgaaca tctcattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
    10981 gaaaacaggc cggaaatgtc ggtcgttcag tgacaaaatt accgttgatg ggaaaatcct
    11041 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcca ggaccagttc
    11101 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
    11161 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
    11221 gcgcttacga tggtacagtg ttcccaccta acattgaagg tggtcgtttt gtctcatggc
    11281 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataaacct gatctggtca
    11341 tcaaactcaa tgttgacctt gaagttgcct gcgcgcgtaa acccgaccat aaacgggaat
    11401 cgctggcgag gaagattgcc ataacgccac agttaacctt tggcggtgca caactggttg
    11461 atatcgatgc taatcagcca ctggaaaagg tgctgattga tgcagaaaaa gcgattacgg
    11521 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
    11581 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacaaccc accagaaaga
    11641 aaaatcgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
    11701 tgtaacccac cgccgtccac caccatcgtt cctgctacgg cagatgagag tacgtgtgtt
    11761 gtggagacgg gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
    11821 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
    11881 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac tgccatgatg
    11941 atccataccg gtgcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
    12001 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaagt atcagagatg
    12061 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtagctttcc
    12121 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
    12181 cagtgaaact ccgtcacttg cgcgccatca gtcgatgccg caggcaatgg aggttccatc
    12241 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
    12301 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
    12361 ggggcaatcc ccaccagtac cagcattacc aggccgatcc ctttctgccc gtcgttagcg
    12421 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
    12481 ggcttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
    12541 tttttcgtcc cgctccagaa acggcgcagc aggaaaatca gaccacccgc aatgaccagg
    12601 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
    12661 gcatccatca ccgatgagcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
    12721 ccaatcaagg tgtgcgaact ggaggccggt aaaccaaaga accacgttcc caggttccag
    12781 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgagttga ccccatattc
    12841 agcaacaaat cggttggtaa catatggaca atggcatagg caacgctaag tccgcccaat
    12901 aacacgccaa aaaaattaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
    12961 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
    13021 tagaacaaca caaatgccag agcaagtaat aacaaaagcc ctgtgtatat atcaaggcca
    13081 acaaataaat ttagcatata ttagattacg acattttgaa tttacgaacg gacgcattat
    13141 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagcgtgtt
    13201 att
//
LOCUS       DAPQZD010000136        13116 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPQZD010000136 DAPQZD010000000
VERSION     DAPQZD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.138"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            155..490
                     /locus_tag="RO454_003727"
     CDS             155..490
                     /locus_tag="RO454_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDY0935493.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            650..1093
                     /gene="yjdN"
                     /locus_tag="RO454_003728"
     CDS             650..1093
                     /gene="yjdN"
                     /locus_tag="RO454_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDY0935494.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1226..2014
                     /gene="phnC"
                     /locus_tag="RO454_003729"
     CDS             1226..2014
                     /gene="phnC"
                     /locus_tag="RO454_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDY0935495.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2039..3055
                     /gene="phnD"
                     /locus_tag="RO454_003730"
     CDS             2039..3055
                     /gene="phnD"
                     /locus_tag="RO454_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0935496.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3160..3939
                     /gene="phnE"
                     /locus_tag="RO454_003731"
     CDS             3160..3939
                     /gene="phnE"
                     /locus_tag="RO454_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDY0935497.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3960..4685
                     /gene="phnF"
                     /locus_tag="RO454_003732"
     CDS             3960..4685
                     /gene="phnF"
                     /locus_tag="RO454_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDY0935498.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4686..5138
                     /gene="phnG"
                     /locus_tag="RO454_003733"
     CDS             4686..5138
                     /gene="phnG"
                     /locus_tag="RO454_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDY0935499.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5135..5719
                     /gene="phnH"
                     /locus_tag="RO454_003734"
     CDS             5135..5719
                     /gene="phnH"
                     /locus_tag="RO454_003734"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDY0935500.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5719..6783
                     /gene="phnI"
                     /locus_tag="RO454_003735"
     CDS             5719..6783
                     /gene="phnI"
                     /locus_tag="RO454_003735"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDY0935501.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6776..7621
                     /gene="phnJ"
                     /locus_tag="RO454_003736"
     CDS             6776..7621
                     /gene="phnJ"
                     /locus_tag="RO454_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDY0935502.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7618..8376
                     /gene="phnK"
                     /locus_tag="RO454_003737"
     CDS             7618..8376
                     /gene="phnK"
                     /locus_tag="RO454_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDY0935503.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8487..9167
                     /gene="phnL"
                     /locus_tag="RO454_003738"
     CDS             8487..9167
                     /gene="phnL"
                     /locus_tag="RO454_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDY0935504.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9164..10300
                     /gene="phnM"
                     /locus_tag="RO454_003739"
     CDS             9164..10300
                     /gene="phnM"
                     /locus_tag="RO454_003739"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDY0935505.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGKIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVEREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10300..10857
                     /gene="phnN"
                     /locus_tag="RO454_003740"
     CDS             10300..10857
                     /gene="phnN"
                     /locus_tag="RO454_003740"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDY0935506.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10844..11278
                     /gene="phnO"
                     /locus_tag="RO454_003741"
     CDS             10844..11278
                     /gene="phnO"
                     /locus_tag="RO454_003741"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDY0935507.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11280..12038
                     /gene="phnP"
                     /locus_tag="RO454_003742"
     CDS             11280..12038
                     /gene="phnP"
                     /locus_tag="RO454_003742"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDY0935508.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12066..12422)
                     /locus_tag="RO454_003743"
     CDS             complement(12066..12422)
                     /locus_tag="RO454_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935509.1"
                     /translation="MKRFPLFLLFTLFTLFTLFTLFTLFTVPAQADIIDDTIGNIQQA
                     INDAYNPDRGRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQER
                     QQLEDEERRMEDEYGR"
     gene            complement(12491..12940)
                     /gene="rpiB"
                     /locus_tag="RO454_003744"
     CDS             complement(12491..12940)
                     /gene="rpiB"
                     /locus_tag="RO454_003744"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDY0935510.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2938 a         3917 c         3614 g         2647 t
ORIGIN      
        1 tttctttttg cgtttccttt caaatgaaaa cgatcgtcat ctaaaatcag cagtaccccc
       61 gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc cgttttttgg
      121 cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact gcccaaaatg
      181 caactccgaa tacacctacg aagataacgg catgtacatc tgcccggaat gtgcctacga
      241 atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag atgctaacgg
      301 caatctgctg gctgacggcg acagcgttac catcattaaa gatctgaaag tgaaaggtag
      361 ctcttcgatg ctgaaaattg gcaccaaagt aaaaaacatc cgcctggttg aaggcgacca
      421 taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaagtctg agtttgtgaa
      481 aaagaactga ttgtattgtg atcggtaggc cggataaggc gctcgcgccg catccggcaa
      541 cggtggcgac tgcctgatgc gacgctgacc gcgtcttatc aggcttccct ctcatctgta
      601 taaatttcga actacactta actggcttct cttaactgag gtcaccatca tgccgttaag
      661 tccctacctc tcttttgccg gtaactgttc cgacgcgatt gcctattatc aacgtacgtt
      721 gggcgcggaa ctgctctata aaatcagctt cggcgaaatg ccaaaatcaa cgcaggacag
      781 cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgccc acgccaatgt
      841 gcgcattgca ggaagcgaca tcatgatgag cgatgctata ccgtcaggaa aagccagcta
      901 ctccggcttt acgctggtgc tcgattctca acaggtggaa gaaggaaaac gctggtttga
      961 taaccttgcc gccaacggaa aaatcgaaat ggcctggcag gaaacgttct gggcgcatgg
     1021 ctttggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtca tcaaacaaca
     1081 gccaacgcaa taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa
     1141 ctcttcatct gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga
     1201 gccacttttt tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa
     1261 aaccttcaat cagcatcagg cgctgcacgc ggttgatctg aacattcatc atggtgaaat
     1321 ggtggctctg cttgggccgt cgggttccgg aaaatccacc cttttacgtc acttaagcgg
     1381 tttgattacc ggcgataaat ctgtcggtag ccatatcgag ctgctgggcc gcacagtcca
     1441 gcgcgaaggc cgcctggccc gcgatatccg caaaagccgc gccaacaccg gctacatctt
     1501 tcaacaattc aacctggtga atcgcctgag cgtactggag aacgtgctga ttggcgcgct
     1561 cggcagcacg ccattctggc gaacctgttt tagctacttc acccgcgagc agaaacagcg
     1621 cgcgttacag gcgctgaccc gcgttggcat ggtgcacttt gcccatcaac gcgtttccac
     1681 actctccggc ggccagcaac agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa
     1741 ggtcattctc gctgacgaac ccatcgcctc gctggacccg gaatcggcac gcatcgtgat
     1801 ggacaccctg cgcgacatca accagaacga cggcattacc gtggtcgtca cgctgcatca
     1861 ggtggattac gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtctt
     1921 ctacgacggc tgcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat
     1981 taaccgcgtc gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat
     2041 gaacgctaag ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt
     2101 aaatccggca tacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga
     2161 atcacagcaa aacctgaaac cgcaatggac gccgttctta caggatatgg agaagaagct
     2221 gggcgtaaag gtcaacgcct tctttgcccc ggactacgcg ggcattatcc aggggatgcg
     2281 cttcaataaa gtggatatcg cctggtacgg caacctgtcg gcgatggaag cggtggatcg
     2341 cgccaacggt caggtcttcg cccagacggt cgcggcggat ggatcgccgg gttactggag
     2401 cgtgttgatc gtcaacaaag atagcccgat caacaacctg aacgatctgc tggcgaagcg
     2461 gaaagatctc accttcggca atggcgatcc taactctacc tccggcttcc tcgtccccgg
     2521 ttactacgtc ttcgctaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc
     2581 cgggcatgaa accaacgccc tcgccgtcgc taacaagcaa gtggatgtcg ccaccaacaa
     2641 caccgaaaac ctcgacaagc tgaaaacctc cgcgccggag aagctgaaag aactgaaggt
     2701 gatctggaaa tcaccgctga tccctggcga tccgatcgtc tggcgcaaaa atctttccga
     2761 aaccactaaa gacaagatct acgacttctt tatgaattac ggcaaaacgc cggaagagaa
     2821 agcggtgctg gagcgcctgg gctgggcgcc gttccgcgcc tccagcgact tgcaactggt
     2881 gccgattcgc cagctcgcac tgtttaaaga gatgcaaagc gtgaaggaca acaaagggct
     2941 gaacgaacag gacaagctgg caaaaaccac cgccattcag gcgcaactgg atgatttgga
     3001 ccgcctgaac aacgcgttaa gcgcaatgag ttcggtgagt aaagcggtgc agtaaatcgt
     3061 aggtcggata agacgcccgg cgtcgcatcc gacaatgtgc agacgttgat gccggatgcg
     3121 gtgcaagcac cttatccggc ctacagaccg gagccaaaca tgcaaaccat caccatcgcc
     3181 ccacccaagc gcagctggtt ctcgcttctg agctgggccg ttgttatcgc cgtgctggtc
     3241 gtctcgtggc agggcgcgga aatggccccg cttacgctga ttaaagatgg cggcaacatg
     3301 gcgacgttcg ccgccgactt cttcccgccc gatttcagcc agtggcagga ttacctcacc
     3361 gaaatggccg tcacgctgca aatcgccgtc tggggcaccg cgctggcggt ggttctctcc
     3421 atccccttcg gcctgatgag cgctgaaaac ctggtaccgt ggtgggtcta ccaacctgta
     3481 cgccgcctga tggacgcctg ccgcgccatt aacgaaatgg tcttcgccat gctgttcgtg
     3541 gtcgccgtcg gcctcggccc gttcgctggc gtgctggcgc tgtttatcca caccaccggc
     3601 gtgctctcca agctgctttc cgaagcggtg gaagcgatcg aacccggccc ggtggagggc
     3661 attcgcgcca ccggtgccaa caagctggaa gagatccttt acggcgtact accgcaggtc
     3721 atgccgctgc tgatttccta ctctctctat cgcttcgaat ccaacgtccg ctcggcgacc
     3781 gtcgtcggta tggttggcgc gggcgggatc ggcgtcacgc tgtgggaagc gattcgcggt
     3841 ttccagttcc agcaaacctg cgccctgatg gtgctcatca tcgtcacggt cagcctgctg
     3901 gatttcctct ctcaacggtt gcgtaagcac tttatctgat aagcgaggca ttgatatcta
     3961 tgcacttgtc tacacatccg accagctacc caacacgcta tcaggagata gccgcaaaac
     4021 ttgagcagga gcttcgtcaa cactaccgct gcggcgacta tcttcccgcc gagcagcaac
     4081 tggcggcgcg ctttgaggtg aaccgccaca ccctgcgccg cgccatcgac caactggtgg
     4141 aaaaaggctg ggtacagcgc cgtcagggcg tcggcgtact ggtgctgatg cgcccgttcg
     4201 attacccgct caacgcccag gcgcgtttta gccagaacct gctggaccag ggcagccatc
     4261 ccaccagcga aaaattgcta tcggtgctgc gccccgcctc cggtcacgtc gctgacgcgc
     4321 tggggataac cgagggggag aacgtcatcc acctgcgcac cctgcgtcgg gtcaacggcg
     4381 tcgcgctctg tttaatcgac cactactttg cggacctcac cctctggccg acgctgcaac
     4441 gtttcgacag cggttcgctg cacgattttc tgcgcgagca aacggggatt acgctacgac
     4501 gcagtcagac gcggatcagc gcccgtcgcg cccaggccaa agagtgccag cgtcttgaaa
     4561 tcccgaatct gtcgccgctg ctgtgcgtgc gcacccttaa ccaccgtgac ggtgaaagca
     4621 gcccggcgga gtactccgtc agcctgacgc gcgccgacat gattgaattc actatggagc
     4681 actgaatgca cgcagatacc gcgacccgcc agcactggat gtccgtgctg gcgcacagcc
     4741 aaccggctga actggcagcc cgcctgaacg cgctgaacat caacgccgat tatgaggtga
     4801 tccgcgccgc tgaaactggc ctggtgcaga ttcaggcgcg aatgggcggc accggcgaac
     4861 gcttttttgc tggcgacgcc acgctcaccc gcgccgccgt gcgtctgact gacggcacgc
     4921 tcggctatag ctgggtgctg gggcgcgaca aacagcacgc cgaacgttgt gcgctgattg
     4981 atgcgctgat gcagcaaaac cgccactttc aaaacttatc agaaaccctt atcgccccgc
     5041 tggacgctga ccgtatggca cgcattgccg cgcgccaggc cgaagtgaac gccagccggg
     5101 tcgacttctt tacgatggtt cgcggagaca acgcatgacc ctggaaaccg cttttatgct
     5161 tcccgtgcag gatgcccagc acagttttcg tcgcctgtta aaggccatga gcgagccggg
     5221 cgtgattgtc gccctgcatc agctcaagcg cggctggcag ccgctgaata tcgccaccac
     5281 cagcgtgctg ctgacgctgg ccgataacga cacgccggtg tggcttgccg cgccattgag
     5341 caacgatatc gtcaatcaga gcctgcgttt tcataccaac gcgtcgctgg ttaatcagcc
     5401 ggaacaggcg accttcgcgg taacagatga ggcgatctcc agcgagcagc tcaacgccct
     5461 ttccaccggc actgccgttg caccagaagc gggcgcaacg ctgattttac aggtcaccag
     5521 cctgagcggc gggcgtatgt tgcgcctcac cggcgcaggt attgccgaag aacggatgat
     5581 cgccccgcaa ctgccaaagt gcattcttca tgaacttacc gagcgcccgc acccgttccc
     5641 gctcggcatc gacctgatcc tgacctgcgg cgagcgcctg ctggctattc cgcgaaccac
     5701 tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg gcgaaaaggc gatcgacgcc
     5761 gcccacgccc tgcaagagag ccgacgccgg ggcgatactg atttgcctga actgagcgtc
     5821 gcccagattg aacagcagct taacctcgcg gtagatcgcg tgatgaccga aggcggtatt
     5881 gccgaccgcg agctggccgc gctggcgctg aaacaggcca gcggcgataa cgtcgaagcg
     5941 atttttctgt tgcgcgccta ccgcaccacg ctggcgaagc tggcggtcag tgagccgctc
     6001 gacaccaccg ggatgcgtct cgaacggcgt atttcagcaa tttataaaga cattcccggc
     6061 ggccaactgc ttggccccac ctacgactac acccatcgcc tgctcgattt caccctgctg
     6121 gcaaacggcg aaacgccaac gctgaccacc gctgacagcg aacagcaacc gtcgccacac
     6181 gtcttcagcc tgctggcgcg tcaggggctg gcgaagtttg aagaagatag cggcgcacaa
     6241 ccggacgaca tcacccgcac gccgccggtt tatccctgtt cgcgttcttc ccgtctacaa
     6301 caattaatgc gcggtgacga aggctatttg ttggcgctgg cctactccac ccaacgcggt
     6361 tacgggcgca atcacccgtt cgcaggcgaa attcgcagtg gctatatcga tatatttatt
     6421 gtgccggaag agctgggatt tgcggtgaac gtcggcgaat tgttgatgac cgagtgtgaa
     6481 atggtcaacg gttttatcga cccgccggat gaaccaccgc attttacacg cggctatggg
     6541 ctggtgttcg gcatgagcga gcgcaaagca atggcgatgg cgctggttga ccgtgcgttg
     6601 caggcaccgg aatacggcga gcacgcaaca ggtccggcac aggatgaaga gttcgtgctg
     6661 gcccatgccg acaacgtcga agccgcaggc tttgtttcgc atctcaaact cccgcactac
     6721 gtcgatttcc aggccgaact ggagctactc aaacgtctgc aacaggagca gaaccatggc
     6781 taatctgagc ggctacaact ttgcttacct cgacgagcag accaaacgca tgatccgccg
     6841 cgctatctta aaagcggtgg caatccccgg ttatcaggtg ccgtttggcg gacgcgagat
     6901 gccgatgccc tacggctggg gaaccggcgg cattcagctc accgccagcg tgattggcga
     6961 aagcgacgtg ttgaaggtga ttgaccaggg cgcagacgac accaccaacg ccgtgtcgat
     7021 tcgtaacttc tttaagcgcg tgaccggggt gaacaccacc gagcgcacgg acgatgcgac
     7081 acttatccag acgcgtcacc gcatacccga aacgccgctg accgaagatc agatagttat
     7141 cttccaggtg ccgatccccg aaccgctgcg ctttattgag ccgcgtgaga cggaaacgcg
     7201 caccatgcac gcgctggagg agtacggcgt gatgcaggtg aagctgtatg aagatatcgc
     7261 ccgcttcggt catatcgcca ccacctacgc ctatccggtg aaggtgaacg ggcgctacgt
     7321 gatggacccg tcgccgatcc cgaaattcga taacccgaaa atggacatga tgcccgccct
     7381 gcaactgttt ggcgcggggc gcgagaagcg catctatgcc gtgccaccgt ttacccgcgt
     7441 ggaaagcctc gatttcgatg atcatccgtt caccgtccag cagtgggatg aaccatgcgc
     7501 catctgcgga tcgacccaca gctatcttga tgaagtggtg ctggatgacg ccggaaaccg
     7561 catgtttgtc tgctccgata ccgattattg ccgccaacag agcgaggcca aaagccaatg
     7621 aatcaaccgt tactttcggt caataacctg acccaccttt atgccccggg caaaggcttc
     7681 agcgatgtct cttttgattt atggccggga gaagttctgg gcattgttgg ggagtccggt
     7741 tccgggaaga ccacgctact gaaatcaatt tccgcccgcc tgacaccaca gcaaggggaa
     7801 attcgctacg agaaccgttc gctgtacgcg atgagcgagg ccgaccgccg tcgcctgctg
     7861 cgcaccgaat ggggtgtggt gcatcagcat ccgcttgacg gcctgcgccg ccaggtgtcg
     7921 gcaggcggca atatcggcga acggttgatg gcaaccggag cgcgtcatta cggcgatatt
     7981 cgcgccaccg cgcagaaatg gctggaagag gtggagattc ccgccaaccg gatcgacgac
     8041 ctgccgacca ccttttccgg cggcatgcag caacgtttgc agattgcccg caacctggtg
     8101 acgcatccga agctggtgtt tatggatgaa cccactggcg ggctggatgt gtcggtacag
     8161 gcgcgcctgc tggacctgct ccgcggtctg gtgatggagc ttaacctggc ggtagtgatt
     8221 gtcacccacg atttaggcgt cgcccgtctg ctggcggacc gtttgctggt aatgaagcaa
     8281 gggaaagtgg tggagagtgg gttaaccgac cgcgtgctcg acgacccgca ccatccgtat
     8341 acccagttgc tggtgtcatc ggttttgcag aattgagctg gtgccggatg cggcgtaaac
     8401 gccttatccg gcctacaaat gcgctccccg taggtcggat aagacgcatc agcgtcgcat
     8461 ccgacacccg aaccacgagg cgaaaaatga ttaacgtaca aaacgtcagt aaaaccttca
     8521 tcctgcacca gcaaaacggc gtgcgcctgc ctgtattaca aggcgcctca ctcaccgtca
     8581 acgccggcga atgcgtggta ctccacggcc attccggcag tggaaaatca actctgctac
     8641 gctcgctgta tgccaactat ctacccgacg aagggcaaat ccagatcaaa cacggtgatg
     8701 aatgggtcga cctggtcacc gcgccagcgc gcaaagtggt ggagatccgc aaaaccactg
     8761 tcggctgggt aagccagttt ctgcgcgtca tcccacgtat ttcggcactg gaagtggtga
     8821 tgcagccgct gctcgatacc ggcgtaccgc gtgaagcctg cgccgctaaa gccgcgcgtc
     8881 tcctcacccg cctgaacgtg cctgaacgcc tgtggcacct tgcgccatcg accttctccg
     8941 gtggcgaaca gcagcgtgtc aacatcgccc gtggctttat cgtcgactac cccattctgc
     9001 tgctcgacga acctaccgcc tcgctggacg ctaaaaacag cgccgccgta gtggaactga
     9061 tccgcgaagc caaagcccgc ggcgcggcca tcgtcggtat cttccatgac gaaaccgtac
     9121 gcgagcaagt cgccgaccgc ctgcacccaa tgggagtctc ttcatgatta tcaataacgt
     9181 caaactggtg ctggaaaacg aggtggtaca cggttctctg gagatgcagg acggcaaaat
     9241 ccgcgccttt gccgaaagcc agagtcacct gtcagaagca atggacggcg aaggcggctg
     9301 gctgctaccg gggctgattg agctgcatac cgataatctg gataaattct tcaccccgcg
     9361 cccgaaagtt gactggcctg cccactcggc gatgagcagc cacgacgcgc tgatggtggc
     9421 gagcggcatc accaccgtgc tggacgccgt ggcgattggt gatgtacgcg acggcggcga
     9481 tcggctggag aatctggaga agatgatcaa cgccatcgaa gagacgcaga aacgcggcgt
     9541 caaccgtgcc gagcaccgtc tgcacctacg ctgcgaactg ccgcatcaca ccacgctgcc
     9601 gctgtttgaa aaactggtcg agcgagaacc cgtgacgctg gtgtcgctga tggatcactc
     9661 accgggccag cgccagttcg ccaaccgcga aaaatatcgt gaatattatc agggcaaata
     9721 ctcgctcacc gatgcgcaga tgcaacagta cgaagaggag caactggcgc tcgccgcacg
     9781 ctggtcacag ccgaatcgcg aatccatcgc cgcaatgtgc cgcgcgcgac acattgcgct
     9841 cgccagccac gatgacgcca cgcacgccca cgtagcccaa tcccatcagc ttggcagcgt
     9901 gatcgccgaa tttcctacca cattcgaagc ggcagaagcc tcgcgcaagc acggcatgaa
     9961 cgtactgatg ggcgcgccga atatcgtgcg cggcggttcg cactccggca acgtggcggc
    10021 cagtgaactg gcgcagcttg gcctgctgga tatcctctct tccgactact accccgccag
    10081 cctgctggat gcggcgtttc gcgtggccga tgaccagagc aaccgcttta cgctaccgca
    10141 ggcggtgagg ctggtgacca aaaacccggc ccaggcactg aatctccagg atcgcggggt
    10201 gattggcgag ggcaaacgcg ccgacctggt gctggcgcat cgcaagggca atcacattca
    10261 tattgaccac gtctggcgtc agggtaaaag ggtgttctga tgacgggaaa actgatttgg
    10321 ttaatgggtc cgtccggctc cgggaaagac agcctgctgg cggaactccg ccagcgggaa
    10381 caaacccagc tactggtagc gcatcgctac atcacgcgtg atgccagcgc cggaagtgaa
    10441 aatcatatcg ccttaagcga gcgggagttt tttacccgcg cggggcaaaa tctgttggcc
    10501 ttgagctggc atgctaatgg tttgtattac ggtgtcggca ttgagatcga cctctggctg
    10561 cacgccggat tcgacgtcgt ggttaacggc tcacgcgccc acctgccgca ggcgcgggca
    10621 cgttatcaat cggcactgct gcccatctgt ttacaggttt cgccggagat cctgcgccag
    10681 cgcctggaaa accgtggccg cgaaaacgcc agtgaaatta acgcccggct ggcgcgcgcc
    10741 gcccgctata ctcctcagga ttgtcttacg ctcaataatg acggcagcct gcgccagtcg
    10801 gtcgacaagc tgctgacgct gattcatcag aaggagaaac accatgcctg cttgtgagct
    10861 tcgcccggct acgcagtacg acaccgacgc agtttacgcg ctgatttgtg agctaaaaca
    10921 ggcggagttt gaccaccacg cgtttcgcgt ggggtttaac gccaatctgc gcgatccgaa
    10981 catgcgctac catctggcgc tgctggatgg cgaagttgtc ggcatgatcg gcctgcattt
    11041 gcagtttcat ctgcatcatg tcaactggat cggcgaaatt caggagttgg tggtgatgcc
    11101 gcaggcgcgc ggcctgaacg tcggtagcaa gttgctggcg tgggcagaag aagaagcccg
    11161 ccaggccggg gccgaaatga ccgagctttc gaccaacgtg aagcgccacg acgcgcaccg
    11221 tttctatctg cgcgaaggct acgagcagag ccatttccgc ttcaccaaag cgctgtaaca
    11281 tgagcctgat cctcacgctc accggcactg gcggcgcaca gggcgttccc gcatggggct
    11341 gtgagtgcgc ggcctgcgcc agagcgcggc gctcaccgca gtatcgccgc cagccatgca
    11401 gcggcgtcgt gaagtttaac gacgcgatca ccctgatcga cgccgggctg cacgatctcg
    11461 ccgatcgctg gtcgcccgga tcgttccagc agtttttgct gacgcactac catatggatc
    11521 acgtgcaggg gctgtttccg ctgcgctggg gcgtgggcga cacaatcccg gtctacggtc
    11581 caccggatga gcagggctgc gacgatctgt ttaaacatcc gggcctgctt gatttcagcc
    11641 acacggtgga gccgtttgtg gtgtttgatt tgcaggggtt gcaggtcacg ccgctgccgc
    11701 ttaaccattc gaaactcact ttcggctatt tactggaaac ggcgcacagc cgggtggcgt
    11761 ggctatctga caccgcaggc ctgccggaaa aaacgctgaa atttttacgc aataatcagc
    11821 cgcaggtaat ggtgattgat tgcagccacc cgccgcgcga ggatgcaccg cgtaatcact
    11881 gtgatttaaa taccgtgctt gcgctgaatc aggttatccg ctcgccacgg gtgattctga
    11941 cccatatcag ccaccagttt gatgcgtggc tgatggaaaa tgcactgccg tcggggtttg
    12001 aggcggggtt tgatgggatg gagattgagg tggagtgagg gaaaataccc gcgccggccc
    12061 ctacctcaac gcccatactc atcttccatt ctccgctctt catcttccag ttgctgacgc
    12121 tcctgatcaa gctggcgctg gcgttcgtcc agctgcctgc ggcgatcttc aaactggcgg
    12181 cggcggtcat catattgtct gcgccgatcg tcgctcactt cacgctgcca gccgtcgtcg
    12241 cgcgaatctt catagtctcg cccacggtca gggttataag cgtcgttaat cgcctgctga
    12301 atattgccaa tggtgtcatc gataatatcg gcctgggccg gaacggtgaa cagcgtgaac
    12361 agcgtgaaca gcgtgaacag cgtgaacagc gtgaataaaa gaaatagcgg aaagcgtttc
    12421 attagccaac ctcaaaaaga aactctatcc acattagtca ttactcatcc atgcaagtag
    12481 tggatgaatc tcaatttctc cgctgctcta ttgccgtaat cgcctccacg cgttgttgat
    12541 gacgaccgcc ttcgtactgt gcgcccagcc acgcatcgac aatcattttt gccagttcga
    12601 ggccaactac tcgtgaacca aaagccagca cgttggtgtc gttatgctgc cgcgaaagtt
    12661 gcgcggaata aggttcgcta caaacgaccg cgcgaattcc ggcaaacttg ttcgccgcta
    12721 tcgaaatacc gacgccagtg ccacaaatca aaatcccgcc atcagcctct ccgccagcaa
    12781 cggccagtgc gacttgactg gcgtaatgcg gataatcagt acgctctgac gaccaggttc
    12841 ctttatcaat cacttcaacg ccacgctcaa ctaaatgtgc cactatttca tgttttaaaa
    12901 tgaaaccgac atgatcacag ccaaatgcaa tctttttcat tcatcttccc cgctcgcgca
    12961 aaacttcaca ataaaaaaac atttgtgatt taaatgaatt aaaaacaccc aaaatcgatg
    13021 aaatcagcct tcccgatctg ttttacagag ttacatctgg cagcgcaata gcgttaaaga
    13081 cactcactat ccggcaacat ctatcatcta ataaac
//
LOCUS       DAPQZD010000137        12899 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPQZD010000137 DAPQZD010000000
VERSION     DAPQZD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.139"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..314
                     /locus_tag="RO454_003745"
     CDS             <1..314
                     /locus_tag="RO454_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDY0935511.1"
                     /translation="DQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLK
                     NEWVPATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAEASF
                     S"
     gene            397..654
                     /locus_tag="RO454_003746"
     CDS             397..654
                     /locus_tag="RO454_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935512.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1211..1978)
                     /gene="fecE"
                     /locus_tag="RO454_003747"
     CDS             complement(1211..1978)
                     /gene="fecE"
                     /locus_tag="RO454_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDY0935513.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1979..2935)
                     /gene="fecD"
                     /locus_tag="RO454_003748"
     CDS             complement(1979..2935)
                     /gene="fecD"
                     /locus_tag="RO454_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDY0935514.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2932..3930)
                     /gene="fecC"
                     /locus_tag="RO454_003749"
     CDS             complement(2932..3930)
                     /gene="fecC"
                     /locus_tag="RO454_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDY0935515.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3927..4829)
                     /gene="fecB"
                     /locus_tag="RO454_003750"
     CDS             complement(3927..4829)
                     /gene="fecB"
                     /locus_tag="RO454_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDY0935516.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4874..7198)
                     /gene="fecA"
                     /locus_tag="RO454_003751"
     CDS             complement(4874..7198)
                     /gene="fecA"
                     /locus_tag="RO454_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDY0935517.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7285..8238)
                     /gene="fecR"
                     /locus_tag="RO454_003752"
     CDS             complement(7285..8238)
                     /gene="fecR"
                     /locus_tag="RO454_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDY0935518.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRILTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8235..8756)
                     /gene="fecI"
                     /locus_tag="RO454_003753"
     CDS             complement(8235..8756)
                     /gene="fecI"
                     /locus_tag="RO454_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDY0935519.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9048..9745
                     /locus_tag="RO454_003754"
                     /pseudo
     CDS             9048..9745
                     /locus_tag="RO454_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(9905..10036)
                     /locus_tag="RO454_003755"
                     /pseudo
     CDS             complement(9905..10036)
                     /locus_tag="RO454_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10318..11676
                     /locus_tag="RO454_003756"
     CDS             10318..11676
                     /locus_tag="RO454_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDY0935520.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(11915..>12899)
                     /locus_tag="RO454_003757"
     CDS             complement(11915..>12899)
                     /locus_tag="RO454_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDY0935521.1"
                     /translation="AEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIA
                     GPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHG
                     YVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKG
                     IHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEE
                     ALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGE
                     RGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         2926 a         3516 c         3618 g         2839 t
ORIGIN      
        1 gcgatcaagg cagtcattat acgagcaggc agttccggca gttactgtgg cgataccgga
       61 tcaggcagag tatgagtcgg cgtggaaact gctgggataa cagcccaatg gagcgcttct
      121 tcaggagtct gaagaacgaa tgggtgccag cgacgggcta tgtaagcttc agcgatgcag
      181 ctcacgcaat aacggactat atcgttggat attacagcgc actaagaccg cacgaatata
      241 atggtgggtt accaccaaac gaatcagaaa accgatactg gaaaaactct aacgcggagg
      301 ccagttttag ttgaccacaa cagactacct gaagggagcc gcggtcgcct ggcagttgca
      361 gtagcaggag atcatccagc cgcagtacag gtcacgatga ctctggttaa tgataccggc
      421 tttgaccccg tattttccgg ctctatcgct gaatcatggc gtcagcagcc gtgcacacca
      481 tcctattgtt gtgactggga ggctgccacc atgcttcgcg ctttccctct ggcgaaaaag
      541 ggagaaggac gggcccgtct gccttcactt tatgccagct tcggtaagct gggtgagaca
      601 ccgactcatg aagatatcat tgataacaat cgatccatca actggcctgt ataacgtggc
      661 tgccggtgat taagaaagct gcacctacct aagtagtagc aaacgcacac tttttagaaa
      721 aatcgatggt cagaaactgg attagcaatt ccgttccagg gttgcttttg atttacgttg
      781 gcgtctgatc attgatttat cctcaaaagc ccaacctcat tggtaatgaa ccagctccgt
      841 gaatgtccgc tctggcacag agcgaaattt tttgatctcc cccctgaaat ctaaacttag
      901 tcatgtcacg tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt
      961 tatacagaaa tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg
     1021 aaagccgcgc ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa
     1081 agcaagcgga tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca
     1141 gtctgaacag gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg
     1201 cctgtgcaat ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt
     1261 ccgcttccac gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc
     1321 cttgcgccat aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct
     1381 gattaaggtc gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca
     1441 accgcatcag gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata
     1501 caacgggcgt attctgggcc aggaccattg ccagaaatgc gcgctggcgc tgaccgccgg
     1561 aaagctcggt taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat
     1621 taactcgtgc attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac
     1681 catacgaaac cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca
     1741 gcgaaagcct gcgggccaac tggcgcgatg agagcatatt tatgggatta tcgccgagaa
     1801 atacggtgcc agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc
     1861 cgcaaccgtt aggaccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt
     1921 cgttaagtac cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt
     1981 atcgcattct cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc
     2041 caaccgggag ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca
     2101 gcaacgcacc tgtcagggcc gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc
     2161 tacgcatcat atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc
     2221 cggtagatgt catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca
     2281 caccgagcgt ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac
     2341 tcaggctcag cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt
     2401 cacggcccca taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg
     2461 agagcatcag ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg
     2521 ccagcttcat cggctggtgg gtctttgcca gcatcttcag caatatcaac cccgccatgc
     2581 cgcccgcaaa ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag
     2641 cccccacaga ggccaggctg gcggcatggt taacaccgag aatatccggt gatgccagag
     2701 ggttgcgcac aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca
     2761 gtgccagcag caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg
     2821 cctgccagtc ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata
     2881 acagcgcaca gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg
     2941 cctcctcaca agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg
     3001 cagatctccg gggaaggcca gtgcgcgtgc gagtacatct gccagcagca tcagcgtggc
     3061 ccccagcagc atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc
     3121 cagatgtggc accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc
     3181 aaccagaagc agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag
     3241 cgtatgggcg gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagcaacac
     3301 gacagggact gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg
     3361 ggacactccg cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag
     3421 ggtgatgcgg gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt
     3481 gtttctgtca tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac
     3541 gccgcccccg catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag
     3601 cgcgctggta agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga
     3661 ggccattggg ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc
     3721 gatcagaacg gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag
     3781 cgttggcgtg tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc
     3841 cgagtagcaa aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca
     3901 cagcagcacc gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag
     3961 atttttaccg tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag
     4021 gtgttactgt cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc
     4081 ggatcttgtt gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag
     4141 gcaggattga ccgccagcag ttgctccagg ccgatggacg gcatggacgc acccgccatc
     4201 gcagcgggaa cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta
     4261 tgcaggttga attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc
     4321 tggctggccc actgcgccat cctctcttta tgttgttcca ggcgtgcctg catctctcgc
     4381 tttttaccca ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg
     4441 ttgcgggact taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca
     4501 tggcgactgc tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc
     4561 ggctgcgcgc gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg
     4621 atgcgttttg catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc
     4681 gcatcggcga acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac
     4741 gtgccgtgtt cgtcctgaac cgtggcggca aaggcgtggc tgatcaccag cagcaggcct
     4801 gcaaaaacaa aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc
     4861 gggcgttaac acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg
     4921 catagatgcc tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt
     4981 ttttcacacc gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc
     5041 gtgcgcccca gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca
     5101 ccgtattggc gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc
     5161 agtttcctgg cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca
     5221 gattgccgta ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt
     5281 agatggaaac gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt
     5341 ccagcccggt atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt
     5401 cgtactgatt gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt
     5461 cgtagcgggt accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca
     5521 cagccttgcc aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa
     5581 gattccagct gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc
     5641 tcacttcttc gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac
     5701 gcatacccgg cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt
     5761 gcgcctcggt gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc
     5821 cgctgctggt ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc
     5881 ccacgcccac ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa
     5941 taccgcgcac ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc
     6001 cgctgcgcag ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct
     6061 ggaactggta gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg
     6121 tggattgcca gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt
     6181 caccgtcgta atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc
     6241 ttttcagcat caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg
     6301 tgccggagta gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt
     6361 tgtgcgtctc tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg
     6421 cctcgatacc aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc
     6481 tctgcggtcc gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc
     6541 cgagcgaaac gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga
     6601 tgccgtccat caggacggtc gagcggctgg tgaggcgcgg gttcaggccc cggatgccaa
     6661 agttcatcgc caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga
     6721 tgcggttaag tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga
     6781 tcacgtcacg cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt
     6841 cgccgaccac ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctat
     6901 tatttcccag cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct
     6961 cgacgtcgta atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa
     7021 ccgagagggt aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg
     7081 gtgcgatatt aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc
     7141 tcaggcgaat ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac
     7201 cttccccatc atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga
     7261 caatcgaaat aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat
     7321 agactgaatt ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt
     7381 cagcgggaac gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc
     7441 gttgcggtaa cgggttagcg tggctatcac ctcacccagt ggtttatcgc tgaagctcag
     7501 gatgtccttc gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga
     7561 ggcgctgaac tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc
     7621 gagaagcact tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg
     7681 gacggtaaat tctgtcccta aagcagtgag ctggccctga cgggtcagga tacggaaggg
     7741 gcgttgcagg gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt
     7801 gcgctgatgc gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga
     7861 gccatcttcc agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag
     7921 accttcgccg gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag
     7981 taatcctttc atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac
     8041 gtcgccaggc acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg
     8101 gttatcctga tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg
     8161 ctcgccgctt agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc
     8221 ggttaacaaa ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct
     8281 ttcgccacgt attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg
     8341 tatgtcagac catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg
     8401 tccagcatgc tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa
     8461 ggcgctcccc cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt
     8521 cggcgaaaca ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg
     8581 cggatcgtcg agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca
     8641 tctgcatcaa aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt
     8701 gtgccataaa gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg
     8761 agtgcatcaa aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa
     8821 ttggaaataa aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa
     8881 ggtaggtata aaatgggatg gagcctctgc ttctggcatg tgtcggtcag aatgactcat
     8941 gatgtggtct gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatgc
     9001 tgccaactta ctgatttagt gtatgatggt gattttaagg tgcttgcgtg gcttccattt
     9061 ccatcagatg tccttcctgc tccgctactg aaggcgtggt gcgtaacggc aaaagcactg
     9121 ccggacatca gcgctatctc tgctctcatt gccgtaaaac atggcaacta cagttcactt
     9181 acaccgcctc tcagcccggt acgcaccaga aaatcattga tatggccatg aatggcgtcg
     9241 gatgtcgcgc cagtgcacgc attatgggcg ttggcctcaa cacggtttta cgtcacttaa
     9301 aaaactcagg ccgtagtcgg taacctcgcg catacaaccg ggcagtgatg tgattgtctg
     9361 cgctgaaatg gacgaacagt ggggctacgt cggtgctaaa tcacgtcagc gctggctgtt
     9421 ttacgcgtat gacaggatac ggaggacggt tgtggcgcac gtcttcggtg aacgcactct
     9481 ggccacactg gagcgtcttc tgagcctgct gtcggccttt gaggtcgtgg tatagatgac
     9541 ggatggctgg ccgctgtatg aatcacgcct gaagagaaag ctgcacgtta tcagcaagcg
     9601 ttacactcag cgcattgagc gacataatct gaatctgaga caacatctgg caaggctggg
     9661 acggaagtta ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
     9721 tctgaacata aaacactatc agtaagttgg agtcattacc tcctcccgaa ctcaaagcta
     9781 tcatacagat aattactggt taaccctata tagggcattt taaagtgtga tcttatccag
     9841 caataatgga cacgcggcta agtgaaccgc cccgggtttc ctggagagtg ttttatctgt
     9901 gaactcaggc tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg
     9961 ccggtgggat atggccctgc ctttccagca atcgtcgatt gttataccag tccacccacg
    10021 tgagtgtggt cagctcatca tactgacacg tgcattaccc ctaccgcgat aatgcaaccg
    10081 tacaatatag ggcgtttcct aaacgcagcc ctcatcactc caatccaaca agacacagca
    10141 tttttgatca cgaagaaaga caaataattt ccgtaaatgt ttccgttgtt acaaataaca
    10201 acagtgtttt acatttcttt ggcctcaggc ccgcgcatgt tgccattcga tgtcattttc
    10261 ctgactcgtt tcttctttat ggtgtagtgt gatcttcact ttatggatgc taacaatatg
    10321 aaaagaaaaa ttattcccgt gctaatcggt tgtacgctct ctttctctgc cctggcggca
    10381 caacctacgg ctgagcgcta tgtcgtcagt tttccagaag gcacgcacgt gaactacgcc
    10441 ggcgcatttg ccagtgcatt cccgaacggc cttccggttg ggataggttc tggcctgctg
    10501 ttcaccggta aacagggcga tgccctgaca ttcgcaacca ttaccgatcg cggtccgaac
    10561 gcggattcac cgaaagaggg gaaaaacgaa actaaaattt tcgttacgcc agatttcgcg
    10621 ccgctgctga tgacgattcg tgtacaaaac ggcaaagcgg aggccatcga tccccgtcct
    10681 ctgcatgacg ataaaggcgc gatcaacggt ctgccgctgg caagcgacgt gattggctcc
    10741 accaatgaag tggccttcag cgacacgtta catagactga agggggataa tcgcgggctg
    10801 gatacggaag ggatcactcc ggatggtaaa ggaggttact ggctgtgcga tgagtatggc
    10861 ccgttcctga ttaacatcga cagtaaaggg aaaatcctgg caatccacgg tccgcaggcg
    10921 gcggaagggg aaaaagcgat tgcgggcggt ttgccaaata tcctcaaatg gcgtcaggcg
    10981 aaccgcggct ttgaaggcct cacccgtatg ccggacgggc gtattattgt cgccgtgcaa
    11041 agtacgctgg atatcgacgc taagagcaag aaaaaggcgc tgtttacgcg tctggtgagc
    11101 ttcgatccgg caagcggaaa aaccgcgatg tacggttatc ctatcgacag cgcggcctac
    11161 agcaaaaaca gcgacgctaa aatcggagac atcgtggcgc tcgataacca gcacatcctg
    11221 ctgatcgaac agggaagaga taaaaacaac agaatgcgta acctgattta cgaggtcgat
    11281 ctcaataagg cgagcgacct gtcaggcttc gacaagccgg gtgagtatcc ggagtttgat
    11341 gatgagaaaa ccctgtcaca gcgcggcatt acccttgcgc agaaaacgca ggttgttgat
    11401 ctgcgctcct tgggctggca gcaggagaag gctgaagggt tggcgctgat cgacagcaaa
    11461 acgctggcgg tggcgaacga taacgacttt ggcgtgaagg tggcgatgca acatccggtc
    11521 gagggcaaga cattcaagga ttatcgggtg aacgcggaag gaaagctgac gcttgacgac
    11581 aagcaggttg aaacgaccct cagagtgaag ccactggaga aaccggagtc tgacagtgaa
    11641 ctgtggattg tgacgctgcc ggaagcgttg aaataagttt ataaggtacg gacagcagta
    11701 tcgcttagtt tttcaaattc atgtatgtga acaatattgg ggttccggcg tagaccccca
    11761 atgaaagcgt caccacgtga aaacatgcct gcacggttta cacgtcatct catggtggca
    11821 gccggttaac tttcctgcta caccagtgga aaaaggccat aagcagtccg aacaaaagta
    11881 agcgtacagc gagggccgta ttgacgggga tgtgttattc agctggcagt gctatgcgcc
    11941 acggaagcag ttcgctgacc cggttgaccg gccagtctgc tatgacgcca ggcacatggc
    12001 gaaggtagct ttctggatcc acgtcattca gtttgcacgt cccgatcagg ctgtacagta
    12061 gcgctccccg ctcaccacca tggccagagc cgaagaacag ggtcttcatc ttttcacgca
    12121 accagctttc cagggatttc aacagcggtt tcgtttttcg ctgacgttca gcaagccgct
    12181 gctctgccgg cattcccctt atatccgcct ctatggcgta caactgaccg atctgctcta
    12241 gggcttcttc cgtcagtgct gacgggatgc ggacgtgcac atcgtggatc tttcggcggg
    12301 catgagccca gcaggcagct tccgttatcc caccattgcg atacagctcg ttgaacccgg
    12361 cgtacgcatc cgcttgcagc acaccgctga agcaagcaag atgagtctgc ggatggatgc
    12421 cttttctgtc cgggctgtaa gcgaaccaca ctgcaggtgc caacgctgac ccggcattgc
    12481 ggtcatcacg aacatacgcc cacaaccgcc cggtcttcgt cttcttatta cccggcagca
    12541 gtacctggac cggggtatca tcggcatgga gtttgccgtc agtcatgaca tagccatgaa
    12601 gcgcctcttc cagcggagac agcagccggc agcatgcatc cacccagccc gacagcagtg
    12661 aacggctcag ctccacacct tgccggccgt atatttctga ctggcaatac agcggggtgt
    12721 gctctgcata cttcgaggtc agcacgcggg ccagcagccc cggtccggcg ataccccgct
    12781 cgatgggccg cgaaggtgca ggtgcctgca cgatggcatc gcactgagta caggcatgtt
    12841 tttcccgtac cgtccggata acccggaagg cgctacgcat caactccagc tgttcggcg
//
LOCUS       DAPQZD010000138        12898 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPQZD010000138 DAPQZD010000000
VERSION     DAPQZD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.140"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            172..1230
                     /gene="citC"
                     /locus_tag="RO454_003758"
     CDS             172..1230
                     /gene="citC"
                     /locus_tag="RO454_003758"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDY0935522.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1245..1541
                     /gene="citD"
                     /locus_tag="RO454_003759"
     CDS             1245..1541
                     /gene="citD"
                     /locus_tag="RO454_003759"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDY0935523.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1538..2446
                     /gene="citE"
                     /locus_tag="RO454_003760"
     CDS             1538..2446
                     /gene="citE"
                     /locus_tag="RO454_003760"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDY0935524.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2457..3989
                     /gene="citF"
                     /locus_tag="RO454_003761"
     CDS             2457..3989
                     /gene="citF"
                     /locus_tag="RO454_003761"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDY0935525.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3993..4544
                     /gene="citX"
                     /locus_tag="RO454_003762"
     CDS             3993..4544
                     /gene="citX"
                     /locus_tag="RO454_003762"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDY0935526.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4519..5397
                     /gene="citG"
                     /locus_tag="RO454_003763"
     CDS             4519..5397
                     /gene="citG"
                     /locus_tag="RO454_003763"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDY0935527.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5448..6911
                     /gene="citT"
                     /locus_tag="RO454_003764"
     CDS             5448..6911
                     /gene="citT"
                     /locus_tag="RO454_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDY0935528.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7025..7831
                     /gene="rna"
                     /locus_tag="RO454_003765"
     CDS             7025..7831
                     /gene="rna"
                     /locus_tag="RO454_003765"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDY0935529.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8061..8471
                     /gene="rnk"
                     /locus_tag="RO454_003766"
     CDS             8061..8471
                     /gene="rnk"
                     /locus_tag="RO454_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDY0935530.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8827..9255
                     /gene="uspG"
                     /locus_tag="RO454_003767"
     CDS             8827..9255
                     /gene="uspG"
                     /locus_tag="RO454_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDY0935531.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9376..10941)
                     /gene="ahpF"
                     /locus_tag="RO454_003768"
     CDS             complement(9376..10941)
                     /gene="ahpF"
                     /locus_tag="RO454_003768"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDY0935532.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11112..11675)
                     /gene="ahpC"
                     /locus_tag="RO454_003769"
     CDS             complement(11112..11675)
                     /gene="ahpC"
                     /locus_tag="RO454_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDY0935533.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12047..12793
                     /gene="dsbG"
                     /locus_tag="RO454_003770"
     CDS             12047..12793
                     /gene="dsbG"
                     /locus_tag="RO454_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDY0935534.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
BASE COUNT         3064 a         3402 c         3263 g         3169 t
ORIGIN      
        1 atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac
       61 ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcaatttgc aacattgaac
      121 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      181 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      241 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      301 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      361 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      421 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      481 ctgttccgcc agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg
      541 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      601 gggaacaaaa ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      661 ctgattcaac aggctgcggc acaatgcgac tggttgcatc tgtttttagt caaagaagat
      721 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      781 ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgcaac gttcccttgc
      841 tacttcatta aagaacagag cgtcattaac cattgctaca ccgaaattga tctgaagatt
      901 ttccgtcagt acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc
      961 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
     1021 atctccgcac cgcccatcga actagttgaa attgagcggc tgcgttacca ggagatgccg
     1081 atatccgctt cccgggtacg tcaactgctg gcaaaaaacg atctcacggc tatcgcgccg
     1141 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1201 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1261 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1321 cgcaggatat cgacctgcaa atcaacagca gcgttgagaa acagtttggc gatgcaattc
     1381 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1441 atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1501 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1561 acgtaaaact cgcacccgcc gcagtatgtt gtttgtgcct ggtgctaatg ccgcgatggt
     1621 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtagc
     1681 attgcgtgaa aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgcttta
     1741 tcgcgatatt gaaaccattg tgcgtgtaaa cgcgctggat tccgaatggg gtgttaacga
     1801 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1861 tcaggatgtt ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga
     1921 acccggcagt accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     1981 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggcgcag aagactatgt
     2041 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg ctcgctgctc
     2101 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2161 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2221 caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca
     2281 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2341 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2401 tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2461 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg
     2521 aatgcgatct tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc
     2581 gcgatcgcaa actgtgcgcc gatctggaag aagcgattcg tcgctctggt ttacaggacg
     2641 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2701 tggacgtcat cgcgaagatg ggctttaaaa acctgactct ggcctccagc tccctgagtg
     2761 attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2821 ctggcctgcg cggtccactg gcggaagaaa tctcccgtgg tctgctggcg gaaccggtgc
     2881 aaatccactc tcacggcgga cgtgtgcatc tggtacagag cggcgaactg aatatcgatg
     2941 tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tactccggta
     3001 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg
     3061 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3121 aggttgatct gatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg
     3181 ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3241 ttgttaactc tggctacttc aaagaaggtt tctccatgca gaccggcacc ggcggcgctt
     3301 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3361 tcgccctcgg cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca
     3421 aactgctgga tgtgcagagc tttgacagcc aagctgcgca atcgctggcc cgtaacccca
     3481 atcacatcga aatcagcgcc aaccaatacg ctaactgggg gtcgaaaggc gcatcggttg
     3541 atcgtctcga cgtggtggta ctgagtgcgc tggaaattga cacccagttc aacgttaacg
     3601 tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga
     3661 ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgacgctgg
     3721 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3781 acggcatcgc tgttaacccg gcacgtccgg agctggcaga acgtctgcag gaagcgggga
     3841 tcaaagtggt ttctattgag tggctgcgtg aacgtgcgcg tctgctgacc ggtgaaccac
     3901 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3961 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4021 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4081 acgcctggct caagcgccat cctgttccac tggtctcctt taccgtggtt gcgccagggc
     4141 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4201 ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4261 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4321 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4381 gcgacattct ctcccgccgc gattattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4441 aaagcgcagc tgtttgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4501 tggaggcgct gctaaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4561 acgtcattaa ttgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaac
     4621 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4681 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4741 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tcaacggatt acgcccaatt
     4801 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaagga
     4861 agcatttttt ctttagggct actgtgtgcg gcaattggcc gtttgcttca gctcaaccag
     4921 ccggtaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat
     4981 cgcgaactgc gtaccaataa ttcacgactg acggcaggtc aacggttgta ccagcagctt
     5041 ggccttaccg gcgcacgcgg tgaagccgaa gcaggttatc cactggtaat caaccacgcc
     5101 ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc actgctcgat
     5161 accttgctcc tactgatggc taccaacggc gataccaacg ttgcatcgcg cggtggcgag
     5221 gggggcctgc gctggctaca gcgcgaggcg caaacactat taaacaatgg gggcatccga
     5281 acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5341 agtccaggcg gcagtgccga cctgctgatt cttacctggt ttttagcaca gatttaatta
     5401 tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa
     5461 aagataatat atggaaacta ttggcgcccc tggtggtgat gggtgtcatg tttcttatcc
     5521 ctgtacctga cggtatgccg ccgcaggcgt ggcattactt tgcagtgttt gtggcaatga
     5581 ttgtcggcat gatcctcgag ccaattccgg caacggcgat cagttttatt gcggttacta
     5641 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5701 ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat
     5761 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggccgtc
     5821 gtattgccct tttcctggtg aaattcatgg ggaaacgcac gctgacgttg ggttatgcga
     5881 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5941 gtacggtttt tccggtcatt aaaaacctgc cgccgctgtt taaatcattc ccgaacgatc
     6001 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     6061 gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca
     6121 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6181 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6241 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacgc
     6301 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6361 gtgaagtcat taatgctact gcggtaggtc tgctggcagt ttcgctgatg ctggcgctgc
     6421 acgttgtgcc gtggaaagac attactcgct ataacagcgc atggaacacg ctggtcaacc
     6481 tggcaacgct ggttgttatg gcgaatggcc tgacccgttc tggctttatc gactggttcg
     6541 ccaacacgat gagtacacac ctggaaggct tctcaccaaa cgcaacggtg attgtactgg
     6601 ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6661 tgctgccggt tattctggcc gtcggtaaag ggattccggg cgtaccaatg gaacaactgt
     6721 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccttat gcaaccggtc
     6781 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6841 caatcttcgg ggtgatttac atctctatgc tgctgttggt tggctggccg attctcgcca
     6901 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca
     6961 cctggcgggc ttttttatga tttaatagat aatattcagt gtatgtatga gttccacacc
     7021 cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc
     7081 ttctgccaac gccgtggcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct
     7141 ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg
     7201 agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttttga ccgtacatgg
     7261 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7321 gcgcttcggt tgcgctactc gcccaatccc gaatctgcca gaagcacgcg ccagccggat
     7381 gtgttcatcg ccggaaaccg ggttatcgct ggaaacggca gctaaactaa gtgaagtcat
     7441 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7501 ctttggtttt gatccagacg catacttcgg cacgatggta cgcctgaatc aagaaattaa
     7561 agagagcgaa gccggaaaat tccttgcgga taactacggt aaaacagtga gccgccgtga
     7621 ctttgatgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7681 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7741 caacgctccg ctttctgcca actcattctt gccacaacca cacccaggca actgtggcaa
     7801 aacctttgtg attgataaag caggttatta atcctctgat ttacaaaaca cgctaattcg
     7861 tcttccataa accatgacga ttaagattta tacataatgc gctacattga ggaattccct
     7921 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
     7981 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt
     8041 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
     8101 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
     8161 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
     8221 tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca
     8281 cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg
     8341 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
     8401 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
     8461 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
     8521 tcctctttcc gctcaaatat caaccagatt tatcattttg ctcttaagcg cagcattcta
     8581 atctgaactg gttattcatc tgcggaacct catatgagcg aagctctcgg tatcactgca
     8641 ttttatatat tcatagcagc aattattgtt gctgcagtgc tataccttga acaacgctgg
     8701 tagcgcctgg ttttatttat tttccagctc aatagcaaat tcagatcaaa tcagcctcgc
     8761 aaattataga ttcataacca ataatgtgct gttatgtata catgcaaaaa caaggagaga
     8821 atgcttatgt acaaaacaat catcatgcca gtggatgttt tcgaaatgga attgagcgac
     8881 aaagcgattc gccacgctga attcctcgcc caggatgacg gagttattca tctacttcac
     8941 gtactaccgg ggtcagccag cctgagcctg caccgttttg ccgctgatgt acgtcgtttt
     9001 gaagagcatc tgcaacatga agcagaagaa cgtttgcaaa cgatggtcag ccacttcacc
     9061 atcgatcctt cccgcattaa gcaacatgtc cgttttggta gtgtgcggga tgaagttaac
     9121 gagttagcga aagaacttga tgcagatgta gtagtgattg gttcgcgtaa cccatcaatt
     9181 tcaactcatc tgttgggttc taacgcctcg agcgtaatcc gccacgccaa tttgccggtg
     9241 ctggttgtgc gttaattgcc agaataagtc tcccgccctg cccggagcca tccctgcagg
     9301 gctttttaat gccataaaaa agccgccagg tttgaccccg gcggctaagc aattacaggt
     9361 gaatcttact tcttcttatg cagttttggt gcgaatcagg tagtcaaaag cactcagaga
     9421 ggctttggca ccttcgccag tggcgatgat gatctgcttg tacggaaccg tcgtacagtc
     9481 acccgctgcg aacacgcctt tgacgttggt ttcgcatttc gcatcaatga taatctcgcc
     9541 catgcgatta cgttcgactg cgccttccag ccagttggtg ttcggcagca gaccaatctg
     9601 gacgaaaata ccggccagtt cgatgttgtg aatatcgccg ctgacacgat cgcgatattc
     9661 cagacctacg actttgctac cgtcgccttt cacttccgtg gtttgcgcat tcagaataat
     9721 gtcgacgttt ttcaggctgc gtagtttgtc ctgcagaacc tggtcggctt tcatttctgg
     9781 cgcaaattcc agcagcgtta cgtgctcaac gatacccgcc aggtcaattg ccgcttccac
     9841 gccggagtta ccgccgccga taaccgctac gcgtttgcct ttaaacagcg ggccgtcgca
     9901 gtgcgggcag taggtcacgc ctttggtgcg atactgatct tcgccaggaa cgttcatgtt
     9961 gcgccatttt gcaccagtcg ccacgataat gctgcgtgct ttcagtaccg cgccagaagc
    10021 tgtttcaatc tgatgcaggc cgccttcaac cgctgccggg atcagtttgc tcgcgctctg
    10081 gctgtcgatc acatcaacgt cgtattcatc aacatgaact ttcagcgcac ctgccagttt
    10141 ctggccttcg gtcttcggta cagaaatgta gttttcgata tcaacggtat cgaggatctg
    10201 accaccaaaa cgttcgccca tcagaccggt acggatgcct ttacgtgcgg agtaaattgc
    10261 tgccgctgca cccgcagggc cggaaccaac gattaatacg tcataagcat cacgcttgtt
    10321 cagctcttct gccgcacgtt tttccgcacc agtatcaatt ttggcaacga tttcagtcaa
    10381 cgtcatgcgg ccctgaccaa actctttccc gtttacgaac actgccggaa cgcccatcac
    10441 gttgcgatcg gtgatttcgt tctggaaggt gccgccgtca attgcagtgt gcttgatgcg
    10501 cgggttcagt acgctcatca ggttcagcgc ctgcaccacg tccgggcagt tgtggcaaga
    10561 gagcgaataa taggtttcga attcaaaatc accgtcaata tggcgaatct gctccagcag
    10621 agactgcgct tctttcgacg gatgaccacc ggtccacaac aacgccagta ccagcgaggt
    10681 gaactcgtgg cccagcgggg aacctgcaaa acgtggcccc tggttggaac cagggttggt
    10741 aatcaggaaa gacggcttac gcaccggcaa gctgttatct tctttaaagg tgactttgtc
    10801 tgacagttct gcgatttcag ccaacagttc cttgatttct gccgatttag cgctgtcatc
    10861 cagcgtggca attaactcaa caggcttggt caatttctca aggtaagcct tgagttgagt
    10921 tttcatattt gtgtcgagca tgtttatctc ctgggcttta aacatcatca tgcaagcggc
    10981 ctctgcggct agctggatgc agcttgcatc atggtgcggg gaagaaaatc gggtgccgca
    11041 gcacccgaag aattaggtga atttccgaag tatctcgcgc cggagttgat agcgcgaaag
    11101 acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    11161 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg tgctgctttg
    11221 attttacgca gcaggtcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    11281 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    11341 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    11401 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    11461 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtaatggtc agcaacgtca
    11521 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    11581 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    11641 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    11701 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    11761 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    11821 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    11881 cagcacctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    11941 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    12001 ttatgccttt tactcgactt ttgcactgac tgaaaaggac aaattaatgt taaaaaagat
    12061 acttttactg gccctgcttc ctgcaatcgc cttcgcagag gaacttcctg ccccagtaaa
    12121 agcgattgaa aaacagggta ttacaatcat caaaacgttc gatgcccctg gaggaatgaa
    12181 aggttatctc ggtaagtatc aggatatggg cgtcaccatc tacctgactc cagatggtaa
    12241 gcacgctatc tctggttaca tgtacaacga aaaaggtgaa aacctgagta acacgcttat
    12301 cgaaaaagaa atttacgcac ccgccggacg tgaaatgtgg caacggatgg aacaatccca
    12361 ctggctcctc gacggtaaaa aagacgcgcc ggtcattgtc tacgtcttcg ccgatccgtt
    12421 ctgcccatat tgtaaacagt tctggcagca ggctcgtccg tgggtagatt ctggcaaagt
    12481 gcaattgaga acattgttgg ttggggttat caagccagaa agcccggcga cagcagcggc
    12541 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggtggcaa
    12601 gcttaagcta agcgtgcctg caaacgtaag cacagagcaa atgaaagtgt taagcgacaa
    12661 cgagaaactc atggacgatc tgggggcaaa tgtcacacca gctatctatt acatgagtaa
    12721 ggaaaatacc ctgcaacaag ccgtcggatt acccgatcag aaaacgctta atatcattat
    12781 gaggaataaa taaaagataa ttcatggatg gttgaaatac atcattgctt ttcaaccatc
    12841 ctaaaaacgc atgaattgct cgtaacccac ctgacagcta tgctatttta gaaaatct
//
LOCUS       DAPQZD010000139        12809 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPQZD010000139 DAPQZD010000000
VERSION     DAPQZD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.141"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(92..1456)
                     /gene="mnmE"
                     /locus_tag="RO454_003771"
     CDS             complement(92..1456)
                     /gene="mnmE"
                     /locus_tag="RO454_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDY0935535.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1562..3208)
                     /gene="yidC"
                     /locus_tag="RO454_003772"
     CDS             complement(1562..3208)
                     /gene="yidC"
                     /locus_tag="RO454_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDY0935536.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3211..3468)
                     /gene="yidD"
                     /locus_tag="RO454_003773"
     CDS             complement(3211..3468)
                     /gene="yidD"
                     /locus_tag="RO454_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDY0935537.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3432..3791)
                     /gene="rnpA"
                     /locus_tag="RO454_003774"
     CDS             complement(3432..3791)
                     /gene="rnpA"
                     /locus_tag="RO454_003774"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDY0935538.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3808..3948)
                     /gene="rpmH"
                     /locus_tag="RO454_003775"
     CDS             complement(3808..3948)
                     /gene="rpmH"
                     /locus_tag="RO454_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDY0935539.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4178..4258)
                     /locus_tag="RO454_003776"
     CDS             complement(4178..4258)
                     /locus_tag="RO454_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935540.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            4555..5958
                     /gene="dnaA"
                     /locus_tag="RO454_003777"
     CDS             4555..5958
                     /gene="dnaA"
                     /locus_tag="RO454_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDY0935541.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5963..7063
                     /gene="dnaN"
                     /locus_tag="RO454_003778"
     CDS             5963..7063
                     /gene="dnaN"
                     /locus_tag="RO454_003778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDY0935542.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7063..8136
                     /gene="recF"
                     /locus_tag="RO454_003779"
     CDS             7063..8136
                     /gene="recF"
                     /locus_tag="RO454_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDY0935543.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8165..10579
                     /gene="gyrB"
                     /locus_tag="RO454_003780"
     CDS             8165..10579
                     /gene="gyrB"
                     /locus_tag="RO454_003780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDY0935544.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10723..11121
                     /locus_tag="RO454_003781"
     CDS             10723..11121
                     /locus_tag="RO454_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDY0935545.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11235..12047
                     /gene="yidA"
                     /locus_tag="RO454_003782"
     CDS             11235..12047
                     /gene="yidA"
                     /locus_tag="RO454_003782"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDY0935546.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12091..12747)
                     /locus_tag="RO454_003783"
     CDS             complement(12091..12747)
                     /locus_tag="RO454_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935547.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
BASE COUNT         3081 a         3332 c         3472 g         2924 t
ORIGIN      
        1 taatgtttaa aaaagggttg aggaataaca ggagtgctaa caatgaagcg aagccgcatc
       61 tgacagtcag aatgcggctt cgcaagcgcg gttacttacc gatacagaag ctggagaaaa
      121 tccgccccag taggtcgtct gaagtaaact ccccggtgat ctcgcttaag ttctgctgcg
      181 ccaggcgcaa ctcttccgcc agcagttcac ccgcccaggc acctaacagt tgcgctttac
      241 cttgctgcaa atgctctgcc gcctgttcca gcgcctgtag gtgacgacga cgcgccagga
      301 agccgccttc catattagtg tcaaagccca tgctctgttt gagatggtta cgcagcacat
      361 ccacgccttc gccagtccgc gccgaaagac gaattaacgc gtgaccgttc acttcgctca
      421 tccccaacgt ttcgccggtg atatcggctt tattgcgcac cacggtgatc ggcagtttcg
      481 ctggcagacg ggcgataaat tccggccaga tctctgccgg atcaacggcg tctgtcgtgg
      541 tgccatcgac cataaacagc acgcggtctg cctgttcaat ttcctgccat gcgcgctcga
      601 taccaatacg ttcaacttcg tcactggctt cacgtagccc ggcggtatcg atgatatgca
      661 acggcattcc gtcaatgtgg atatgctcac gcagcacgtc acgcgtggtt ccggcgatat
      721 cggttacgat tgctgcttca cggcccgcca gcgcgttcag caggctcgat ttaccggcgt
      781 taggacgtcc ggcaatcacc actttcatcc cttcgcgcaa caaactaccc tgacgagctt
      841 cggcgcgtac cgcgtcgaga tcggcaatta cgtcattgag ctgggcttca attttgccgt
      901 cggagaggaa atcgatctct tcatccggga aatcaattgc cgcttcgacg taaatgcgca
      961 agtgggtgag ggcttccacc agatggttga cccgtgcgga gaacgcgcct tgcagcgagt
     1021 ttagcgccga acgggccgcc tgttccgagc tggcgtcgat aagatccgca atcgcctcgg
     1081 cctgggctaa gtcgagttta tcgttcagaa atgcgcgttc ggaaaactca ccagggcggg
     1141 caatccgcag gccgggaatg gtcagaatgc gttttaacag caggtcgagg atcaccggac
     1201 cgccatgacc ttgcagttcc agcacatctt cgccggtgaa cgagttcggg ccagggaacc
     1261 atagcgcaat cccctgatcg agcacgctgc catcggcgtc tttaaacgga agataatcgg
     1321 cgtagcgtgg cttaggcagt ttacccagca cggtttcggc aacttcacgg gctttgaggc
     1381 cggagatgcg caggatgcca acgccgccac gtcccggagg cgtggcctgg gctacgatag
     1441 tgtcattatc gctcatgatg ttcctgttgc tttgtgtggc ggatgtgtgg tgcttatccg
     1501 ccctacgaaa agaaaaaagg cggtcaactg accgccctta ttttagcgaa aactcaccga
     1561 atcaggattt tttcttctcg cggctatgca agccacgttt ttccagacca cggtaaatca
     1621 gctgctgctg aataatggtt accaggttgc tgacgatata gtacagcacc agacctgacg
     1681 ggaaccacag gaagaacaca gtgaagatga ccggcataaa ggtcatgatc ttctgctgca
     1741 tcgggtcggt cacagtggtc ggcgacatct tctgaatgaa gaacatcgtt acgcccatca
     1801 ggatcggcag gatgtagtac gggtcctgtg cagacaggtc gtggatccac agtgcaaacg
     1861 gtgcctgacg tagttcaacg gaacccatca gcatgtagta caacgccagg aagattggca
     1921 tctggatcag cagcgggaag cagccgccca gcgggttaac cttctcagct ttgtacagcg
     1981 ccatcatttc ctggcttatg cgctgtttgt catcgcccag acgctcacgc attgcctgaa
     2041 tcttcggctg cagcatacgc atcttcgcca tggaggtgta ctgcgctttg gtcagcgggt
     2101 acatgatgcc acgaacgata aaggtgatga tgataatgga gaagccccag ttacccacaa
     2161 agctatggat ccatttcagc agtttgaaca gtggctgaga gatgaaccac aaccaaccgt
     2221 aatcaacggt cagatccagg tgcggagcaa cagctgccat tttgtcctga atttccgggc
     2281 caacccacag ggtgctgttc atcgcgccag tctgaccagg ctgaaccagt accggctgag
     2341 atttatagcc gatagcggcg atgccgttac ccagattagc ggtgtagaag ttgttggtac
     2401 cgtcgttatg cgggatccac gccgtcgcga aatactgttg cagcattgcc acccaaccac
     2461 ctttcgaaga gatgttcagg ttttcgttat cggcaatggt atcgaatttg tatttctcgt
     2521 acttctcgtc aggcgtggag tacgccgcac cacggaaggt gtgcagtgcg aagttgctgc
     2581 ttccggtatc gagatgcggt ggcagagtga tggattgctt caactgacca aaggtggaga
     2641 tttccagcgg tttctcgcca gcgttctgca cgttgtagtt gacgttgaca gcgtaatcac
     2701 cacgtttcag gacaaacgtt ttggtaaacg tgttgcctgc cgcgtcggta tacgtcatcg
     2761 gcacctgcag ttcgttttga ccttcagcca gcacataagc gtctttttca acgttatata
     2821 gcggacgcgg gccgttagcc gggttatccg ggccatcacg accggtcaga ccgctctgtg
     2881 cctgataaat aaactgcggt gaagtttcca gcagctggaa cggctgggta gagttcagct
     2941 ctttcgggta agccggcagc agggcctgct caacatcacc accacgggtg ttgatggtca
     3001 gatcaagcac gtcggtctta accgagatca gtttcccctg gccactggcc ggtacgccct
     3061 ggtcggcggc gctacccgct gcggtggtcg ttgtctgcgt ggtctgttgg gcctgaggtt
     3121 gcgggttttt atcctgctcc caggcttgcc agatcatgaa agacacgaac agcaaagcga
     3181 tgactaaaag attgcgttgc gaatccatcg ttagtgttct ctggtatcaa atggtccggg
     3241 cgggacggga tcgtcaccac cagggtgtaa agggtggcat tttaatacgc gtttcaccgt
     3301 caaccaactg ccttttatca ctccaaacct gcgcaatgcc tcaattccgt agcttgaaca
     3361 ggttggagtg aaacgacaat gcggcccgag tagcggacta atcaggcgtt gatagacccg
     3421 aatgagggct atcaggaccc gcgagccagg cgacagtggc ggcgccataa tttttccaac
     3481 gcttccgaga gagcacggtt atcgaggtcg gcaacccctt ttttcgccac caccacgaaa
     3541 tccatagccg ggagttcatg ttggcgcaga cggaagcttt cacgcgtcag acgtttaatc
     3601 cgattgcgtt catgggcgcg tcgaacgttt ttcttggcga ctgtaagacc gatacgggga
     3661 tgccccagcg aattcaggcg gccgagaata gtaatttgcg gcgtgccagc ccgttgtggc
     3721 tgctggaaga cgaatgtgaa ttgactggga gttaacaagc gtaactccct gggaaatgcg
     3781 agcttaacca ctcaggggtt agctttatta cttagaaacg gtcagacgag cgcggccttt
     3841 agcacgacga cgtgccagaa cctgacgacc atttttagta gccatacgag cacggaagcc
     3901 gtgagaacgg ttgcgcttca gtacagacgg ttgaaaagtg cgtttcatgg cgatttctac
     3961 ctaaacttga ataaattcaa tggctttatt ggatatccgc cgaaaaatga aacgatggac
     4021 accgaagcca tgggtgatta aagaggccgg attgtaataa ttgtacactc cggagtcaat
     4081 tctctttcct tatttaccgc gcttttccgc accttttcgc ggggaaaacg tacggcctca
     4141 caccagtgaa aaccagcatg gcgcgccggg tggaggatta tacgggctga cgggtaaagc
     4201 gcaaggatcg tcctggatct ttattagatc gattaagcca ttttttgtct atggtcatta
     4261 aattttccaa tatgcggcgt aaatcgtgcc cggcctgcgg caggatcgtt tacacttagc
     4321 gagttctgga tcatcctgtg gataaatcgg gaaaatctgt gagaaacaga agatctcttg
     4381 cgcagtttag gctatgatcc gcggtcccga tcgttttgca ggatcttgat cgggcatata
     4441 accgcagaca gcggttcgtg cgtcaccctc atgcagggtc ttttcgacgt acgtcaacaa
     4501 tcatgaatgt ttcagcctta gtcattatcg acttttgttc gagtggagtc cgccgtgtca
     4561 ctttcgcttt ggcagcagtg tcttgcccga ttgcaggatg agttaccagc cacagaattc
     4621 agtatgtgga tacgcccatt gcaggcggaa ctgagcgata acacgctggc cctgtacgcg
     4681 ccaaaccgtt ttgtcctcga ttgggtacgg gacaagtacc ttaataatat caatggactg
     4741 ctaaccagtt tctgcggagc ggatgcccca cagctgcgtt ttgaagtcgg caccaaaccg
     4801 gtgacgcaaa cgccacaagc ggcagtgacg agcaacgtcg cggcccctgc acaggtggcg
     4861 caaacgcagc cgcaacgtgc tgcgccttct acgcgctcgg gttgggataa cgtcccggct
     4921 ccggcagaac cgacctatcg ttctaacgta aacgtcaaac acacgtttga taacttcgtt
     4981 gaaggtaaat ctaaccaact ggcgcgcgcg gcggctcgcc aggtggcaga taaccctggc
     5041 ggtgcttata acccgttgtt cctttatggc ggcacgggtt tgggtaaaac tcacctgctg
     5101 catgcggtgg gtaacggcat tatggcgcgc aagccgaatg ccaaagtggt ttatatgcac
     5161 tccgagcgct ttgttcagga catggttaaa gccctgcaaa acaacgcgat cgaagagttt
     5221 aaacgctact accgttccgt agatgcactg ctgatcgacg atattcagtt ttttgctaat
     5281 aaagaacgat ctcaggaaga gtttttccac accttcaacg ccctgctgga aggtaatcaa
     5341 cagatcattc tcacctccga tcgctatccg aaagagatca acggcgttga ggatcgtttg
     5401 aaatcccgct tcggctgggg actgactgtg gcgatcgaac cgccagagct ggaaacccgc
     5461 gtggcgatcc tgatgaaaaa ggccgacgaa aacgacattc gtttgccggg cgaagtggcg
     5521 ttctttatcg ccaagcgtct acgatctaac gtacgtgagt tggaaggcgc gctgaaccgc
     5581 gtcattgcca acgccaactt taccggacgg gcgatcacca tcgacttcgt gcgtgaggcg
     5641 ctgcgcgact tgctggcatt gcaggaaaaa ctggtcacca tcgacaatat tcagaagacg
     5701 gtggcggagt actacaagat caaagtcgcg gatctccttt ccaagcgtcg atcccgctcg
     5761 gtggcccgtc cgcgccagat ggcgatggcg ctggcgaaag agctgactaa ccacagtctg
     5821 ccggagattg gcgatgcctt tggtggtcgt gaccacacga cggtgcttca tgcctgccgg
     5881 aagatcgagc agttgcgtga agagagccac gatatcaaag aagatttttc taatttaatc
     5941 agaacattgt catcgtaaac ctatgaaatt taccgtagaa cgtgagcatt tattaaaacc
     6001 gctacaacag gtgagcggtc cgttaggtgg tcgtcctacg ctaccgattc tcggtaatct
     6061 gctgttacag gttgctgacg gtacgttgtc gctgaccggt actgatctcg agatggaaat
     6121 ggtggcacgc gttgcgctgg ttcagccgca cgaaccaggg gcgacgaccg ttccggcgcg
     6181 caaattcttt gatatctgcc gtggtctgcc tgaaggcgcg gaaattgccg tgcagctgga
     6241 aggtgagcgg atgctggtgc gctccgggcg tagccgtttt tcgctgtcta ccctgccagc
     6301 ggcagatttc ccgaacctcg atgactggca gagcgaagtc gaatttaccc tgccgcaggc
     6361 gacgatgaag cgtctgattg aagcgaccca gttttctatg gcacatcagg acgttcgcta
     6421 ttacttaaac ggtatgctgt ttgaaaccga aggtgaagaa ctgcgcaccg tggcgaccga
     6481 cggccaccgt ctggcagtct gttcaatgcc aattggtcaa tctttgccaa gccattcggt
     6541 gatcgtgccg cgtaaaggcg taattgaact gatgcgtatg ctcgacggcg gcgacaatcc
     6601 gctgcgcgtg cagattggca gcaacaacat tcgcgcccac gttggcgact ttatcttcac
     6661 ctcgaaactg gtggatggtc gcttcccgga ttaccgtcgc gttctgccga agaacccgga
     6721 caagcatctg gaagctggct gtgatctgct caagcaggcg tttgcccgtg cggcaattct
     6781 ctctaacgag aaattccgcg gcgtgcgtct gtatgtcagc gaaaaccagc tgaaaatcac
     6841 cgccaacaac ccagaacagg aagaagcgga agagatcctc gacgttacct atagcggtgc
     6901 ggagatggaa atcggcttca acgtcagcta tgtgctggat gttctgaacg cactgaaatg
     6961 cgaaaacgtc cgcatgatgc tgaccgattc ggtttccagc gtgcagattg aagatgcggc
     7021 cagccagagc gcggcttatg ttgtcatgcc aatgagactg taatgtccct cacccgcttg
     7081 ttgatccgcg atttccgcaa cattgaaacc gcggatctcg ccttatctcc cggctttaac
     7141 tttctggtag gtgccaacgg cagtggcaaa accagcgtgc tggaagccat ctatacgctc
     7201 ggccacggtc gggcgtttcg cagtttgcag attggtcgcg tcattcgcca cgagcaggag
     7261 gcgtttgttc tccacggacg attacagggc gaagagcgtg agacggcgat tggtttaacc
     7321 aaagacaaac aaggcgacag caaagtccgt attgacggta cagacgggca taaagtcgcg
     7381 gagctggcgc acctgatgcc aatgcagctg ataacgccag aagggtttac tttactcaat
     7441 ggcggcccca aatacagaag agcattcctc gactggggat gctttcacaa cgaacccgga
     7501 tttttcaccg cctggagcaa tctcaagcga ttgctcaagc agcgcaatgc ggcgctgcgc
     7561 caggtgactc gttacgaaca gctacgcccg tgggataaag aactgatccc gctggcggag
     7621 caaatcagca cctggcgcgc ggagtatagc gccggtatcg cagccgatat ggctgatacc
     7681 tgtaagcaat ttctccctga gttttctctg actttctctt tccagcgcgg ctgggagaaa
     7741 gagacagagt acgctgaggt gctggaacgt aattttgaac gcgatcgcca gttaacctac
     7801 accgcgcacg gcccgcataa agcggactta cgcattcgcg ccgacggtgc gccggtggaa
     7861 gataccttat cgcgtggaca gcttaagctg ttgatgtgcg ccttacgtct ggcgcaagga
     7921 gagttcctca cccgtgaaag cgggcggcgg tgtctctacc tgatagatga ttttgcctct
     7981 gagcttgatg atgagcgtcg cgggctgctt gccagccgct taaaagcgac gcaatcacag
     8041 gtctttgtca gcgcgatcag tgctgaacac gtaatagaca tgtcggacga aaattcgaag
     8101 atgtttaccg tggaaaaggg taaaataacg gattaaccca agaataaatg agcgagaaac
     8161 gttgatgtcg aattcttatg actcctccag tatcaaagtc ctgaaagggc tggatgcggt
     8221 gcgtaagcgc ccgggtatgt atatcggcga cacggatgac ggcaccggtc tgcaccacat
     8281 ggtattcgag gtggtagata acgctatcga cgaagcgctc gcgggtcact gtaaagaaat
     8341 tatcgtcacc attcacgccg ataactctgt ctctgtacag gatgacgggc gcggcattcc
     8401 gaccggtatt cacccggaag agggcgtatc ggcggcggaa gtgatcatga ccgttctgca
     8461 cgcaggcggt aaatttgacg ataactccta taaagtgtcc ggcggtctgc acggcgttgg
     8521 tgtttcggta gtaaacgccc tgtcgcaaaa actggagctg gttatccagc gcgagggtaa
     8581 aattcaccgt cagatctacg aacacggtgt accgcaggcc ccgctggcgg ttaccggcga
     8641 gactgaaaaa accggcacca tggtgcgttt ctggccaagc cttgaaacct tcaccaatgt
     8701 gaccgagttc gaatatgaaa ttctggcgaa acgtctgcgt gagttgtcgt tcctcaactc
     8761 cggcgtttcc attcgtctgc gcgataagcg cgacggcaaa gaagaccact tccactatga
     8821 aggcggcatc aaggcgttcg ttgaatatct gaacaagaac aaaacgccga tccacccgaa
     8881 tatcttctac ttttccaccg aaaaagacgg tattggcgtc gaagtggcgt tgcagtggaa
     8941 cgatggcttc caggaaaaca tctactgctt taccaacaac attccgcagc gtgacggcgg
     9001 tactcacctg gcaggtttcc gtgcggcgat gacccgtact ctgaacgcct acatggacaa
     9061 agaaggctac agcaaaaaag ccaaagtcag cgccaccggt gacgatgcgc gtgaaggcct
     9121 gattgcggtc gtttccgtga aagtgccgga cccgaaattc tcctcacaga ccaaagacaa
     9181 actggtttct tctgaggtga aatcggcggt tgaacagcag atgaacgaac tgctggcgga
     9241 atacctgctg gaaaacccaa ccgacgcgaa aatcgtggtt ggcaaaatta tcgatgctgc
     9301 ccgtgcccgt gaagcggcgc gtcgcgcgcg tgaaatgact cgccgtaaag gtgcgctcga
     9361 cttagcgggc ctgccgggca aactggcaga ctgccaggaa cgcgacccag cgctttccga
     9421 actataccta gtggaagggg actccgcggg cggctctgcg aagcaggggc gtaaccgcaa
     9481 gaaccaggcg attctgccgc tgaagggtaa aatcctcaac gtcgagaaag cgcgcttcga
     9541 taagatgctc tcttctcagg aagtggcgac gcttatcacc gcgcttggtt gtggtatcgg
     9601 tcgtgacgag tacaacccgg acaaactgcg ttatcacagc atcatcatca tgaccgatgc
     9661 ggacgtcgac ggctcgcaca ttcgtacgct gctgttgacc ttcttctatc gtcagatgcc
     9721 ggaaatcgtt gaacgtggtc acgtctacat cgctcagccg ccgctgtaca aagtgaagaa
     9781 aggtaagcag gaacagtaca ttaaagacga cgaagcgatg gatcagtacc agatctctat
     9841 cgcgctggat ggcgcaacgc tgcacaccaa cgccagtgca ccggcgctgg ctggcgaagc
     9901 gttagagaaa ctggtgtctg agtacaacgc gacgcagaaa atgatcaacc gcatggagcg
     9961 tcgttatccg aaagcaatgc tgaaagagct tatctatcag ccgacgctga cggaaaccga
    10021 cctttctgac gagcaaaccg ttacccgctg ggtgaacgcg ctggtcagcg aactgaacga
    10081 taaagaacag cacggcagcc agtggaagtt tgatgtccat accaatgccg aacaaaacct
    10141 gttcgagccg attgttcgcg tgcgtaccca cggtgtggat actgactatc cgctggatca
    10201 cgaatttatc actggcggcg aatatcgtcg tatctgcacg ctgggtgaga aactgcgtgg
    10261 cttgctggaa gaagatgcat ttatcgaacg tggcgaacgt cgtcagccgg tagccagctt
    10321 cgagcaggcg ctggactggc tggtgaaaga gtcccgtcgc ggcctctcca tccagcgtta
    10381 taaaggtctg ggcgagatga acccggaaca gctgtgggaa accaccatgg acccggaaag
    10441 ccgtcgtatg ctgcgcgtta ccgttaaaga tgcgattgct gccgaccagt tgttcactac
    10501 gcttatgggc gacgccgttg aaccgcgccg tgcgtttatc gaagagaacg ctctgaaagc
    10561 ggcgaatatc gatatttaat ggctttttgt tgctgataaa atgacttagt cgtcagtccg
    10621 ggcggagatc atctccgccc tttttattcc tgcaatccgc aattatcatc ccgcagatct
    10681 atactttttg ggtcaacacg taacgcttta caggtgatta tcatggggct ttttgatgaa
    10741 gttgtcggtg cctttctgaa aggcgatgcg gggaaatatc aggcgatttt gagctgggtt
    10801 gaggagcagg gcggcattca ggtgctgctg gaaaaactgc aaagtggcgg cttaggggcc
    10861 attctctcaa cctggctgag taatcaacag agcaatcaat cagttagcgg cgagcaagtg
    10921 gaatcggcgc tcggcacaaa tgcggtgtcc gatctcgggc aaaaacttgg cgtggatacc
    10981 agtacagctt ccagtttact ggcagaacaa ttgccgaaga ttattgatgc actctcaccg
    11041 caaggtgaag tgtcaccaca agccaataac gatctgcttt ccgcaggtat ggaactgctg
    11101 aaagggaaac tcttccgcta agcaaatggg gagcacgcaa tgcgcgttgc atcccctttt
    11161 ccctcgggtg ttgtactgat ttttgagcgg aatcgcgtta gcatgggtca ggaaccaatc
    11221 tacctgggga actcatggct atcaaactca ttgctatcga tatggatggc acccttctgc
    11281 tgcccgatca caccatttca cccgccgtta aaaatgcgat tgccgcagct cgcgcccgtg
    11341 gcgtgaatgt cgtgctaacg acgggtcgcc cttatgcagg tgttcacaat tacctgaaag
    11401 agctgcatat ggaacagccg ggcgactact gcattactta taacggcgcg ctggtacaga
    11461 aggccgctga tggtagcacc gtggcgcaaa ccgctctcag ctatgacgac taccgtttcc
    11521 tggaaaaact ctctcgcgaa gtcggttctc atttccacgc cctggaccgc accacgctgt
    11581 acaccgccaa ccgagatatc agctactaca cggtgcatga atctttcgtt gccaccattc
    11641 cgctggtgtt ctgcgaagcg gagaaaatgg accccaatac ccagttcctg aaagtgatga
    11701 tgattgatga acccgccatc ctcgaccagg ccattgcgcg tattccgcag gaagtgaaag
    11761 aaaaatacac cgtgctgaaa agtgcgccgt acttcctcga aatcctcgat aaacgcgtta
    11821 acaaaggtac gggtgtgaaa tcactggccg acgtgttagg tattaaaccg gaagaaatca
    11881 tggcgattgg cgatcaggaa aacgacatcg caatgattga atatgcaggc gtcggtgtgg
    11941 cgatggataa cgctattcct tcggtgaaag aagtggcgaa ctttgtcacc aaatctaacc
    12001 ttgaagatgg cgtggcattt gctattgaga agtatgtgct gaattaatca gtgaacgggc
    12061 aaacggcatg aatgcgtttg cccgcagcga ttaacaattt ctggtcgtgc ataaaatcag
    12121 gaactggagt ggtgacgtta ctatatccag cgtaagagtg actggcagca gcgcattaac
    12181 gaggccgcgc gggacgccat ctgttgcttt gtactcataa aatcccaccg taaagggttg
    12241 atggaaaacc atcacgtttg agtaatccgc ttttttattt ttctggtgga ttgagccttt
    12301 caaaccgtcc agcatccatg tacaacgccg ggtatcacaa ataaaaccat attgtgttgc
    12361 cagtgcctta tcttcttcgt tgttccagtc gtaatagagc gtaatagcac cagagaaatt
    12421 cttattactg ctgcttaaaa taaatttcgc gttatcagaa acggtgattc tttcaccatg
    12481 gattgccgga tcgttcagca tgttaaccac ctcatcgccg ccggtggtca atagatagtc
    12541 caacgattca ccaacaaaaa cataaccttt cgtaccgtta ctgtcttcag ccagcgataa
    12601 acccttaatc gtgtctgacc gccattgctc ggcatgtgga cggcttgcag tattggaaac
    12661 caatccccag gtgatacatc cggtcaacgc aaatgccaaa gaacacaaac cggcagcctt
    12721 aaaaaatcct ttaaaattca atttcatgat taatccttta aacaagagtg ctaagccgtt
    12781 gaaagcatac tcttctttcc ctcctgttt
//
LOCUS       DAPQZD010000140        12695 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPQZD010000140 DAPQZD010000000
VERSION     DAPQZD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.142"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            6..464
                     /gene="decR"
                     /locus_tag="RO454_003784"
     CDS             6..464
                     /gene="decR"
                     /locus_tag="RO454_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDY0935548.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            494..2266
                     /locus_tag="RO454_003785"
     CDS             494..2266
                     /locus_tag="RO454_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDY0935549.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            2259..4040
                     /locus_tag="RO454_003786"
     CDS             2259..4040
                     /locus_tag="RO454_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDY0935550.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     SLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4221..4559
                     /gene="glnK"
                     /locus_tag="RO454_003787"
     CDS             4221..4559
                     /gene="glnK"
                     /locus_tag="RO454_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDY0935551.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4589..5875
                     /gene="amtB"
                     /locus_tag="RO454_003788"
     CDS             4589..5875
                     /gene="amtB"
                     /locus_tag="RO454_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDY0935552.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5924..6784)
                     /gene="tesB"
                     /locus_tag="RO454_003789"
     CDS             complement(5924..6784)
                     /gene="tesB"
                     /locus_tag="RO454_003789"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDY0935553.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            7002..7574
                     /locus_tag="RO454_003790"
     CDS             7002..7574
                     /locus_tag="RO454_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDY0935554.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7605..7916)
                     /locus_tag="RO454_003791"
     CDS             complement(7605..7916)
                     /locus_tag="RO454_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDY0935555.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            8079..8175
                     /gene="ffs"
                     /locus_tag="RO454_003792"
     ncRNA           8079..8175
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RO454_003792"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8295..8648
                     /locus_tag="RO454_003793"
     CDS             8295..8648
                     /locus_tag="RO454_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDY0935556.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DIPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8690..10240)
                     /gene="pdeB"
                     /locus_tag="RO454_003794"
     CDS             complement(8690..10240)
                     /gene="pdeB"
                     /locus_tag="RO454_003794"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDY0935557.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWYRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10404..10874)
                     /locus_tag="RO454_003795"
     CDS             complement(10404..10874)
                     /locus_tag="RO454_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDY0935558.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(10990..11541)
                     /gene="maa"
                     /locus_tag="RO454_003796"
     CDS             complement(10990..11541)
                     /gene="maa"
                     /locus_tag="RO454_003796"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDY0935559.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(11715..11933)
                     /gene="hha"
                     /locus_tag="RO454_003797"
     CDS             complement(11715..11933)
                     /gene="hha"
                     /locus_tag="RO454_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDY0935560.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(11959..12333)
                     /gene="tomB"
                     /locus_tag="RO454_003798"
     CDS             complement(11959..12333)
                     /gene="tomB"
                     /locus_tag="RO454_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDY0935561.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         3026 a         3041 c         3433 g         3195 t
ORIGIN      
        1 gggctatgtt agataaaatt gaccgtaagc tgctggcctt gctacagcag gattgcaccc
       61 tctctttgca ggcactggct gaagccgtta atctgacaac caccccctgc tggaagcgtc
      121 tgaaacggct ggaggacgac ggtattctta tcggcaaagt cgccctgctg gatccggaaa
      181 aaataggcct cggcctgacc gcctttgtgc tgataaaaac gcaacatcac agcagcgaat
      241 ggtattgccg ctttgtcacg gtggttaccg aaatgccaga agtgctgggg ttctggcgca
      301 tggctggtga atacgattat ctgatgcgcg tccaggttgc cgacatgaaa cgctacgacg
      361 agttttataa gcgtctggta aacagtgtac cggggctgtc ggacgtcact tccagcttcg
      421 cgatggaaca gattaaatac accacttctt tacccatcga ataaatatcc agaatcaagt
      481 caggacacaa cgcgtgcgat tatttgctca attaagctgg tatttccgtc gggaatggcg
      541 tcgctatctc ggggctgtcg ccttgcttgt cattatcgcg atgctgcaac tggttccgcc
      601 aaaagtggtt ggtattgttg ttgatggcgt gacagaacaa cactttacta ccgggcagat
      661 cctgatgtgg atcgccacca tggtgctgat tgccgttgtg gtttatctcc tgcgttacgt
      721 ctggcgcgta ttgctgtttg gtgcgtctta tcaactggct gttgaactgc gtgaagatta
      781 ttaccgccag ctaagccggc agcatcctga gttttacctg cgtcatcgca cgggcgacct
      841 tatggcccgt gcgacaaatg acgtcgatcg cgtcgtgttt gccgccggag aaggggtgct
      901 gacgctggtg gattcactgg taatgggctg cgctgtgttg attatgatgt ctacgcaaat
      961 tagctggcag ttgaccttat ttgccctgtt gccgatgcca gtgatggcga tcatgatcaa
     1021 gcgcaacggc gatgccttgc atgaacgctt taagctggca caggcggcgt tttccagtct
     1081 taatgaccgc acccaggaaa gcctcaccag tatccgcatg atcaaagcct ttggtctgga
     1141 agatcgtcag tcggcgttat ttgccgcgga tgccgaagat accggcaaaa aaaacatgcg
     1201 ggtggcgcgt attgatgctc gtttcgaccc gaccatctat atcgcgattg gtatggcgaa
     1261 cttgctggcg atcggcggcg gtagttggat ggtggtgcag ggcagtttaa cgctgggcca
     1321 gctcaccagt tttatgatgt atttaggtct gatgatttgg ccaatgctgg cgctggcatg
     1381 gatgtttaac attgtggaac gtggcagcgc ggcgtatagc cgaattcgta ccatgctggc
     1441 cgaagcgcca gtcgttattg atggtagtga taaggtgcca gaaggtcgtg gtgaactgga
     1501 tgtgaatatt cgccagttta cgtatccgca gactgaccat cctgcgctgg aaaacgtcaa
     1561 tttcgccctg aaacccggtc agatgctggg tatctgcggg ccgactggtt ccggcaaaag
     1621 taccctgttg tcgctcattc agcgtcattt cgacgtcagc gagggtgata tccgttttca
     1681 tgatattcct ctgacgaagt tacaactcga tagctggcgt agccgtctgg cggtggtcag
     1741 ccagacacca ttcctttttt ccgacaccgt ggcgaacaac atcgcgttgg gttgcccgaa
     1801 tgccacccag caagagattg aacacgtagc gcggttagcc agcgtacatg acgatatttt
     1861 gcgtctgccg caaggttacg acacagaagt gggcgagcgc ggtgtgatgc tttctggcgg
     1921 acaaaaacag cgtatctcca ttgctcgagc gttattagtc aacgcggaaa tcctcatcct
     1981 tgatgatgcg ctttcagcgg tggacggacg cacagagcac cagatcctgc ataacctgcg
     2041 tcagtggggg cagggaagaa cggtaatcat cagtgcccat cgcctttctg cactgacgga
     2101 agccagtgaa attattgtga tgcagcacgg gcatatcgcc cagcgtggca atcatgatgt
     2161 gctggtacaa caaagcggct ggtatcgcga tatgtatcgc tatcaacaac tggaggcggc
     2221 gctcgacgac gccccggaaa ttcgcgagga ggccgtcgat gcgtagtttt agccaactgt
     2281 ggccgactct caagcgtctg ttagcgtacg gttcaccgtg gcgtaaaccg ttggggattg
     2341 cggtcctgat gatgtgggtt gcggcggcgg cagaagtcag tgggccgctg cttatcagct
     2401 attttatcga caatatggta gcgaaaaata acctgccgtt gaaagtggtt gcagggctgg
     2461 cggcggcgta tgttgggctg caactgtttg cagccgggct acattacgcg cagtcgctgc
     2521 tgtttaatcg ggcggcagta ggcgtagtgc aacagttgcg taccgacgtg atggatgctg
     2581 cgttacgcca gccattaagc gagtttgata cccaacccgt cgggcaggtg atttcccgcg
     2641 tcactaatga cactgaagtg atccgcgatc tctacgttac cgtagtggca actgtcctgc
     2701 gtagcgccgc actggtgggc gcgatgctgg tggcgatgtt cagcctcgac tggcgaatgg
     2761 cgctggtggc gataatgatt ttcccggtgg tgctggtggt aatggtgata tatcagcgtt
     2821 acagcacgcc gattgtccgt cgtgtgcgcg cttatctggc ggatatcaac gacggcttta
     2881 acgaaatcat caatggcatg agcgttatcc agcagttccg tcagcaggcg cgatttggcg
     2941 aacgtatggg tgaggccagt cgttcacact atatggcgag gatgcaaacc ctgcgcctcg
     3001 acggttttct gctgcgtccg ctgctgagtc tgttttcatc gctcattctt tgtggcttgt
     3061 tgatgctgtt tggcttctcc gccagcggca ccattgaagt gggcgtgttg tatgcgttca
     3121 tcagttattt ggggcgactt aacgaaccct taatcgaact gaccacgcag caggcgatgc
     3181 tgcaacaggc tgttgttgct ggtgagcgcg tttttgagct gatggacgga ccgcgccagc
     3241 aatatggcaa tgatgatcgc tcgttgcaga gcggcaccat cgacgtcgat aacgtgtcat
     3301 ttgcttatcg cgatgacaat ctggtgctaa agaacattaa tctctctgtg ccttcgcgca
     3361 actttgtggc gctggtcggg cataccggca gtggcaaaag caccctcgcc agtttattga
     3421 tgggctatta cccgctaacg gaaggtgaga ttcgccttga tggtcgtcca ttaagtacgc
     3481 taagtcacag cgcgctgcgc cagggcgtgg caatggtgca gcaagatccg gtggtgctgg
     3541 cggatacctt cctcgccaac gtgacgctgg gacgtgatat ctctgaagaa cgcgtctggc
     3601 aggcgctgga aaccgtgcag ctggcggagc tggcgcgtag catgagtgat ggtatttaca
     3661 cgctgcttgg cgagcagggg aataatctct cagtcgggca aaagcaactg ctggcactgg
     3721 cgcgcgtgct ggtggagacg ccgcaaatcc tgatccttga tgaggcaacc gccagcattg
     3781 actccgggac tgaacaggcg attcaacatg ctctggcggc ggtgcgtgaa catactacgc
     3841 ttgtggtgat tgctcaccgc ttatcgacta ttgttgatgc cgacaccatt ctggtgcttc
     3901 atcgtgggca agccgtggag cagggcactc accagcaact gctggcggcc cagggacgat
     3961 actggcagat gtatcaactg caacttgcgg gcgaagagct ggcagccagc gtgcgtgaag
     4021 aggaatcatt gagcgcctga atagcgcaat atttcatcgt tggtgcaaaa atgtaacgca
     4081 ctgtgcactg tcatagtgcg ttttcatttt caatcttctt aacttcctgc tctctttctc
     4141 gtttttcatt tctggcacac cgcttgcaat accttcttcg tgtagcagaa ccattaccga
     4201 attctgaccg gaggggatct atgaagctgg tgaccgtgat aatcaaacca ttcaagctgg
     4261 aagacgttcg tgaagcgtta tcttccattg gtattcaggg cctgaccgtc accgaagtga
     4321 aaggttttgg gcgtcagaaa gggcatgccg agctgtaccg gggggcggaa tacagcgtca
     4381 acttcctgcc aaaagtaaaa attgatgtgg cgattgctga tgaccaactc gatgaagtga
     4441 tcgatatcgt cagcaaagcg gcttacaccg gaaaaattgg cgacggcaaa atcttcgtcg
     4501 ctgaattgca acgcgtcatt cgtattcgta ccggcgaagc cgacgaagcg gcgctgtaat
     4561 ctctggcaca cagcaacagg aacgaaaaat gaagatagca acgataaaaa ctgggcttac
     4621 ttcactggcg atgctgccgg gactggtaat ggctgcacct gcggtggccg ataaagccga
     4681 caatgcattt atgatgattt gtactgcgct ggtgctgttt atgactattc cggggattgc
     4741 cctgttttat ggagggctga ttcgtggcaa aaacgtgctg tcgatgctga cgcaggtgac
     4801 cgtgacgttt gcactggtct gtattctctg ggtggtttac ggctactcgc tggcgtttgg
     4861 cgaaggcaac aacttcttcg gcaacattaa cgggttgatg ctgaaaaaca ttgaactgac
     4921 ggcggtgatg ggcagcattt atcagtatat ccacgtggcg tttcagggat cgtttgcctg
     4981 cattaccgta ggcttgatag ttggggcgct ggcggaacga atccgcttct cagctgtgtt
     5041 gatttttgtg gtggtatggc tgacgctctc ttacattccg attgcgcata tggtctgggg
     5101 cggcggtttg ctggcttctc acggtgcgct ggatttcgcg ggtggcacgg tagtgcacat
     5161 taacgccgca atcgccggtc tggtgggcgc gtatctgata ggcaaacgcg tgggcttcgg
     5221 caaagaggcg tttaaaccgc acaacctgcc gatggtcttc accgggactg ccattctcta
     5281 tatcggttgg tttggcttta acgccgggtc agcgggcacg gcgaatgaaa tcgcggcact
     5341 ggcatttgtg aatactgtgg tcgcaaccgc ggcggcaatt cttggctgga tcttcggtga
     5401 atgggcgctg cgtggtaagc cttcactgct gggagcgtgt tctggcgcga ttgccggtct
     5461 ggtcggcgtg acgccagcct gtggttacat tggggttggc ggcgcgttga ttatcggcgt
     5521 ggtagctggt ctggcgggct tgtggggcgt taccatgctc aaacgcttgc tgcgggtgga
     5581 tgatccctgc gatgtcttcg gtgtgcacgg cgtttgtggt attgtcggct gtatcatgac
     5641 cgggattttt gccgccagct cgctgggcgg cgtgggcttc gctgaaggtg tgacgatggg
     5701 ccatcagttg ctggtacagc tggaaagcat cgccattacg attgtctggt ctggtgtggt
     5761 ggcatttatc ggctacaaac tggcggatct gacggttggt ctgcgtgtac cggaagagca
     5821 ggagcgagaa gggctggacg tcaacagcca cggcgagaat gcctataacg cgtaacaagc
     5881 actgcaaaaa acagccggac ggttttcacc tccggctatt tttttaattg tgattacgca
     5941 tcaccccttc ctgaacggtc gaggcaacca gcacgccgtc ctgggtataa aactcaccgc
     6001 gcacaaagcc gcgtgcgctg gacgccgagg tgctttccac gctatacagc agccattcat
     6061 tcaaattaaa cgggcgatgg aaccacatgg aatggtcaat ggtggcaatc tgaatccccg
     6121 gttcgagaaa accgatgccg tgcggctgta gagctaccgg caggaaatta agatcagaag
     6181 cataaccgag cagatactga tgaacgcgca gatcatccgg cacgctacca tttgcgcgga
     6241 tccacacctg acgatgcggt tctgcgacgt gacctttcag tgggttatga aactccaccg
     6301 gacggacttc cagcggacga tcgcagatga atttatcttt cagcactggc ggcagcaggt
     6361 gcgccagcga ttgggcgatt tgcgtttctg aaggcaggcc atcaggcgct ggtgcggacg
     6421 gcattgtttt ttgatgttcg aaacccgctt ctggtgcctg gaaagaggca gtcatataaa
     6481 aaatcggttt gccgttttga atagcagcaa cccggcgggc gctgaagctg ttaccgtcac
     6541 gcagtgtttc gacatcataa ataatcggct tcttactatc gccagggcga agaaagtagc
     6601 tgtgaaaaga atgtaccagc cgctcgtcag ggacggtctc ttttgcagca tacaaggcct
     6661 gacccacgac ctggccgcca aatacctggc gtaaacctaa atcttcactc tggccgcgaa
     6721 agagtccttc ctcaattttt tccagattta acaatgtcag taaatttttt agcgcctgac
     6781 tcatataact ctccagtaac aaagctgccg cagcaagcca aagtgagttg agtataacgc
     6841 aaatttgcca ctggtccgat gggtgcaatg gtctgaatta cgggctaatt acaggcagaa
     6901 atgcgtgatg tgtgccacac ttgttgacgt tactattttg ttaaccactc ttccggcgag
     6961 gaaagttagc ccgctggtgc attgataata aggagaagtg aatgaaactc gtgcacatgg
     7021 ccagtggttt agcggttgcg attgcgttgg cggcttgcgc agataaaagc gcggatattc
     7081 agacgccagc accggctgca aatacgtcta tttcagcaac acaacaacct gctatccagc
     7141 aaccgaatgt ctccggtacc gtttggatcc gtcagaaagt cgcactgccg cctgatgccg
     7201 tgctgaccgt gacactttct gacgcgtcgt tagccgatgc gccgtcgaaa gtgctggcgc
     7261 agaaagcggt gcgtaccgaa ggtaaacagt caccattcag ctttgttctg ccatttaacc
     7321 cggcagatgt tcagccgaac gcgcgtattc tgttgagtgc ggcgattacc gtgaatgaca
     7381 aactggtatt tattaccgat accgttcagc cggtgatcaa ccagggcgga actaaagccg
     7441 acctgacatt ggtgccggtg cagcaaaccg ccgtgccggt tcaggctagc ggtggcgcaa
     7501 cgactaccgt accttcaact tcaccaaccc aggtgaatcc gtcttcggca gttccagctc
     7561 caacgcaata ttaagcgaag ttaaccctct ccgcccggag agggttagta gttccagcga
     7621 taaagctgca agtcgatttg cccgcttccc gacaccatca caccttctgc cagtaatgcc
     7681 tgtcgctgac gctgtaaatc cggtccggtc agtgaaatgg tgccgtggcg attaaccacc
     7741 cggtgccagg gcaaggtgct gccttcgggg agacgcttta acacaccgcc aacctggcgc
     7801 gcggcacggg gcgagcccgc cagtttcgcc acatcaccgt aagtggtgac atagccttcg
     7861 ggaatagcgg ctacgatttg ccagacgcgt tggggaaatg aatcttcttt ttccatcttt
     7921 tcttcctgag gtaatttttc agcataatct ggaaaaacgc ccgagtgaag ccgcattgcg
     7981 caagaaacca gcatctggca cgcaatgggt tgcaattagc cggggcagca gtgataatgc
     8041 gcctgcgcgt tggttctcaa cgctctcaat gggggctctg ttggttctcc cgcaacgcta
     8101 ctctgtttac caggtcaggt ccggaaggaa gcagccaagg cagatgacgc gtgtgccggg
     8161 atgtagctgg cagggccccc acccatttct gcctcctacc atttcgtcaa aaaatcccaa
     8221 catggctaaa ctttaaccac aactgacgtc gcaagaattg tctggctgcg cagtacgctt
     8281 cggaggtatg tctgatgaag tatgttgatg gttttgttgt tgccgttcct gccgataaaa
     8341 aggatgccta tcgggaaatg gccgctaagg ccgcgccatt atttaaagag ttcggggcgc
     8401 ttcgtattgt cgaatgctgg gccagcgata taccggatgg taaagtgacc gattttcgta
     8461 tggcggtgaa agcggaagag aatgaagagg ttgtctttag ctggattgaa tacccttcaa
     8521 aagaggtccg cgacgctgct aatcaaaaga tgatgtcgga cccacggatg aaagagttcg
     8581 gcgagtccat gccgtttgat ggtaagcgaa tgatctatgg cggattcgag tcaatcatcg
     8641 acgagtaggt agcgtgacgg gctgtgcggc gtgccgtcag ccctataatt cacaaatgtt
     8701 gctcggccca gcgtaagaaa tcctctttcg gtaatgcctt gctgtagagc cagccctgac
     8761 cgtagtgcac gccatgctgg cgtagccact cttcttgttt actggtttcg attccctccg
     8821 caaccatttt cagtttcagt gtttttgcca tttcgatgat atgtggcgtg acattcttat
     8881 attccagcgc atcaacgaaa gatttatcga tcttcagaat gtcgacatcc agatcctgta
     8941 aatagcttaa acttgaatac cctgtaccaa aatcatcgag ataaatttca tggcccgcct
     9001 cccggtagcg agaaattatc ggggcgctgg ttttcggatc ggcaaactcg cgttcagtga
     9061 gttcaagcgc gatctgtctg ggattaacct gatagtgatt gatcatttca cgcagcaatt
     9121 gcgggatttt ttccgaggtg agcacggtgg attcaagatt gatcgaaata tgctgctgag
     9181 gatgctgacg cagccagtcg cccatatctt caaagacgct tcttataatc agtagcgtca
     9241 atggctcaga aaggcccgtt tgctgtgcca gcggaataaa actatctggt gacaaccaac
     9301 taccgtctgt ctgcggccag cgcgccagtg cctcagcacc gacaattttg ccattggcta
     9361 aggagacaat cggctgatag tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc
     9421 gatgatgcgg tgactggata cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc
     9481 caatcaccaa cccgagcggt aaccaaataa agacttgccg ataccagcct ttctgcaaca
     9541 ttttcgttga agcccaggtg atgatcgaaa tattcatctc cggtaagggc aggatatcgt
     9601 aaatgattcc attattttcg atatgctcac cgggtgtttt ttgtagcctg gtaataattc
     9661 cctgagcaag ttcatcgctg ctggttatga caacgttatg ggcattgcca ataatggcgg
     9721 catcaatttg ccatgagcta tagggaatga catcaataaa ggaagcgggg tcgatcatga
     9781 cgacataatg tgccgttccc atggcgacca tgtaacggat aatgcctaaa tcgttatgcg
     9841 atgttaacca gacacgataa ccatctttcg aaattttacc tggctcgggg aaggtatcgg
     9901 gcgggctttc atgctccaga gacgaacatt ggggaacgtt gttatcgata taaaccactt
     9961 cctgaatata gcgataactg taagataccc gacgcatttc catgagatgg gcttcgctac
    10021 aggcagcgcc ttgccatctt tccagctctt gcagcgcatc tttcccttgt gtcgccacct
    10081 tattggcccg aatagcgacg cgggaggaat acgtattcag ctcttcaata aatgacgttt
    10141 ctacctgctg atgggccagc cagatgctta agccgacagg cagcaatact gaaagaatca
    10201 gtactcccga aataaggccg accagatgtc gtgttctcac cagcatatcc ttattaacac
    10261 agtgtaggga aagtatatct gattgtggaa aaaggctggc aggagataag gaggtagagc
    10321 tggtatttca tgaagtgatg ggtggaagca atcgttgtca tttgaccagg cgtagagctt
    10381 tacgcctggt atgtacccct aacttaagat gttgattcgg ctcgagcgag ggtaaaaata
    10441 tcgtaaaaag atagttcacc tttacggacg atcatttttt gcagttgttc tttatgaaca
    10501 tccgcgacac gtggagagtt caggatggct tcaaccagcg cggcaggtac aaagcgcgtg
    10561 ttgtaccatt cgccgttata gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg
    10621 tagcgtgggt agacatcgaa aaagaagaaa ccattaagca ggataaataa tacaaccagt
    10681 agccagacag agcctgaaag cgtttcggat tgcaacatca ccgccagggt agcaaaaaaa
    10741 gcaacgtaca taccgataaa cagccccgga tgtttacgga taaaactgat actaaagcgg
    10801 ggtttgttgt cgcgtttttc gcgggtattc agagactcaa tcgtttcggt cagcaggcgt
    10861 tgtatttcgg tcatcgtttg ccttcgtgat atctgcaact ttccagggaa caccctattt
    10921 taacgtggga atagtagagg ggaagtaaca gatttgccac gattttgcat aacagttgag
    10981 aaaagctggt tacaattttt taatgattct ggctggatta ccgcccacga caacgttgtc
    11041 cgggacatct tttgtgacaa ctgcacctga ggctaccacg acgttatcac caatggtcac
    11101 accagggtta atgactgcac gtccgccaat ccagacgtta ttaccgatgg tgacgggttt
    11161 ccccagttca gcaccgctat tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat
    11221 atgaacgcct ggtgccaaca tacagttatc accaatgcga atagggcaga catcaagcat
    11281 cacgcaatcg aagttggcga aaaaattatt gccgagaaaa atgttatagc catagtcaca
    11341 gcgaaacgtt ggctcaatat aagcctctgt cacctgaccg aataaatcag caagaatttg
    11401 ctggcgtaat gtgtgctctt ccgccaggga atgattgtat cggtgaataa gccgacgagc
    11461 gcgcaggcga tcgcgagata acgcctcatc tgccgagtga tacaactcac cagcaatcat
    11521 cttttctttt tctgtgctca ttactgactt ccggttgctg aaaatgcggc aacacagtaa
    11581 gacgaaaagt cagtgaagag aacgttccca ttacatctgt gtgatgaaga tcacataggg
    11641 tatgccttca tggcgaaaaa aagtataaaa ttcttaataa acagccggtt atagctccga
    11701 aagcgagtta tttattagcg aataaatttc catactgagg aagggatctt gtcgtacagt
    11761 ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac cgccagttca
    11821 ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc aattgtctgg
    11881 caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga catacttcta
    11941 cccatggttg taataattct aacaagataa actcgcagga ttctctttcg tcgcattgac
    12001 aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct gcatattaat
    12061 accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct gctcaatgag
    12121 cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa tatgctcaat
    12181 cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc catgattgct
    12241 ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact taagctgtgc
    12301 gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta gcggttgaac
    12361 taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct ctcgtttgtt
    12421 agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa aattacaatt
    12481 aggaaggaag cgggacagga ctcacttttt catactagca ccaacccgca gcaggtaaaa
    12541 gcagtttgct cgaactgttc ataacatttc agaattatcg ccagaagaaa ttggtgacac
    12601 taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa gaattaccgc
    12661 aaataattaa gaatagcctc taaatggtta tggat
//
LOCUS       DAPQZD010000141        12663 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPQZD010000141 DAPQZD010000000
VERSION     DAPQZD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.143"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            187..441
                     /gene="bssS"
                     /locus_tag="RO454_003799"
     CDS             187..441
                     /gene="bssS"
                     /locus_tag="RO454_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDY0935562.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            556..1674
                     /gene="solA"
                     /locus_tag="RO454_003800"
     CDS             556..1674
                     /gene="solA"
                     /locus_tag="RO454_003800"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDY0935563.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1722..1835
                     /locus_tag="RO454_003801"
     CDS             1722..1835
                     /locus_tag="RO454_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDY0935564.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2096..2662
                     /locus_tag="RO454_003802"
     CDS             2096..2662
                     /locus_tag="RO454_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDY0935565.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2666..3241
                     /locus_tag="RO454_003803"
     CDS             2666..3241
                     /locus_tag="RO454_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDY0935566.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3283..4335)
                     /locus_tag="RO454_003804"
     CDS             complement(3283..4335)
                     /locus_tag="RO454_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDY0935567.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4560..5480
                     /gene="lpxL"
                     /locus_tag="RO454_003805"
     CDS             4560..5480
                     /gene="lpxL"
                     /locus_tag="RO454_003805"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDY0935568.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5652..6878
                     /gene="mdtG"
                     /locus_tag="RO454_003806"
     CDS             5652..6878
                     /gene="mdtG"
                     /locus_tag="RO454_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDY0935569.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            6961..7335
                     /locus_tag="RO454_003807"
     CDS             6961..7335
                     /locus_tag="RO454_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HDY0935570.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(7336..7563)
                     /locus_tag="RO454_003808"
     CDS             complement(7336..7563)
                     /locus_tag="RO454_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDY0935571.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7735..10278)
                     /gene="mdoH"
                     /locus_tag="RO454_003809"
     CDS             complement(7735..10278)
                     /gene="mdoH"
                     /locus_tag="RO454_003809"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDY0935572.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10271..11806)
                     /gene="mdoG"
                     /locus_tag="RO454_003810"
     CDS             complement(10271..11806)
                     /gene="mdoG"
                     /locus_tag="RO454_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDY0935573.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12200..>12663
                     /locus_tag="RO454_003811"
     CDS             12200..>12663
                     /locus_tag="RO454_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDY0935574.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCV"
BASE COUNT         3154 a         3254 c         3066 g         3189 t
ORIGIN      
        1 atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa
       61 atttatctgt tcccccttta aaaattcggt gaataccctt acttattggt atactgaagt
      121 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
      181 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
      241 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
      301 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
      361 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttccaggta
      421 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
      481 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
      541 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
      601 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
      661 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
      721 gaaggcgaaa agtatgtccc gctggtcctc cacgcgcaaa tgctgtggga tgaactttcc
      781 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggtccggct
      841 gactccgcat ttctcgccaa cgtcgcccac agcgccgacc agtggcaact caacgttgaa
      901 aagctcgacg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
      961 atcggtttat ttgaaactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
     1021 atccaactgg cgaaggaagc gggctgtgcg cagctgttca actgcccggt caccgcaatt
     1081 cgtcatgacg atgatggcgt aactattgaa acggcagacg gtgagtatca ggcgaaaaaa
     1141 gcgattgtct gcgcgggaac atgggtaaaa gacctactcc cggagctgcc tgtccagccc
     1201 gtacgcaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
     1261 ccggcattta ccggtgagct gcccaatggc gatcaatatt atggttttcc ggcagaaaac
     1321 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
     1381 gttccgtttg cggaagtggc cagcgatggt tcggaagcct tcccgttctt gcgcaatgtg
     1441 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
     1501 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
     1561 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
     1621 gacaaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
     1681 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcga ctgttgcact
     1741 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
     1801 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
     1861 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattcttc ctgcctctac
     1921 agcactcact agaccttcca gatgcttaac ccactaaact tatgaagatt ctttcattat
     1981 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
     2041 ttcattctcc gacacgccat tttctttctc tcgcaaattc gttttggatg tacttatgtc
     2101 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttacatt ggctaagcgc
     2161 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
     2221 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
     2281 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
     2341 ttcgcccatg actcgtcttg cagcaaaagc tggacatctc gcattgtacc tattgctgtt
     2401 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
     2461 tggctggttt gacgtccccg cgaccctctc cgacgctggc gcacaggctg actttgccgg
     2521 tgccctgcat ttttggctcg cgtggagcgt tgtagtactg tccgttatgc acggatttat
     2581 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
     2641 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcttc
     2701 cttgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
     2761 gcacgccttt gttaacttcc gaatccagca tcttggctat agctggttat atggcacctt
     2821 caaagatttc gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa
     2881 tgtgacaatt aacaccacca gcgtcgatac taatcacgcc gaacgcgata aacatcttcg
     2941 cagtgcggat ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt
     3001 gaagaaagac ggtgacgaac tggatattac cggcaatctg acgctgaatg gtgtaaccaa
     3061 gcccgtcaca ctggaagcga aattaattgg tcagggtgac gacccgtggg gcggtaaacg
     3121 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
     3181 aggtccagcg tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
     3241 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
     3301 aatgcccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
     3361 acgtcctgcc cgacggcgtc gctgttcctc tggcggtaac gcgctttctt cgcagcaaat
     3421 ctcactacaa caacctttgt atttttccgc acatactgga cactgaataa acagcagatg
     3481 gcagccatca tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcagtg
     3541 cgcgataatc tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
     3601 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
     3661 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
     3721 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
     3781 aactgctttt ggcagctgct cacggaaggt atctgccggt atttccagcg cgttttcaaa
     3841 gtgccccact tcatactcat agtggttacg catgtcgata aacaatgcat cgggatcgtc
     3901 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
     3961 aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatacgca gtacccagaa
     4021 ggatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccggatcgaa
     4081 ggcatagagc tgtgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
     4141 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagttgata
     4201 taaagcgtca cgggtcacct taggatcggc aatgtggaaa tacttataaa acgaaatggt
     4261 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
     4321 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
     4381 atatagcaat tatcgataat taacatccac acattttacg ctacatttta gcattaaaaa
     4441 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagtcacaaa ttttggttgc
     4501 ggacgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
     4561 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
     4621 tgggtattgg cgtactttgg ttggtcgtgc aattgcccta cccggttatc taccgcctcg
     4681 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
     4741 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
     4801 agaatttcga atccgttggc atgggcctga tggaaaccgg catggcgtgg ttctggccgg
     4861 accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
     4921 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc
     4981 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
     5041 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
     5101 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc
     5161 atgattacgg cccgcgctca agcgttttcg tcccgttgtt tgccgttgag caggctgcga
     5221 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
     5281 cacgccgtaa gccagatggc aaagggtatc agttgattat actgccgcca gagtgttctc
     5341 cgccgctgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
     5401 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
     5461 gcgttccttc acgctattaa atcttccatg ctggatgctt cagaatggca tccggcatta
     5521 ccacagaaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
     5581 gggcgcataa ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac
     5641 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
     5701 tcgtcgcctg gctaggctgt tttcttaccg gtgccgcctt cagcctggta atgcccttct
     5761 tacccctcta cgttgagcag cttggcgtta ccggccactc cgccctgaat atgtggtccg
     5821 gtattgtctt cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac
     5881 tcgccgatcg taaaggccga aaactcatgc tattacgctc tgctctcggc atgggcatcg
     5941 tgatggtgtt gatgggactg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc
     6001 ttggtttact tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc
     6061 gtaataaaag cggctgggcg ctgggtacgc tctccacagg cggcgttagc ggcgcgttgc
     6121 tcggcccaat ggctggcggt ttgctcgccg atagctacgg cttacgtccg gtattcttta
     6181 ttaccgccag tgtgctcata ctctgctttt tcgtcaccct gttttgcatc agagaaaaat
     6241 tccagccggt cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa
     6301 acccgaaact ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacgggct
     6361 caattgcccc cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg
     6421 cctttatcag tggcatgatc gcctcggtgc caggcgtggc ggctctgcta agtgcaccac
     6481 gactcggcaa acttggcgat cgaatcggac ccgaaaagat cctgattacg gcgctgatct
     6541 tttctgtact gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt
     6601 tacgtttttt gctcggtgcc gccgatggtg cactactccc cgccgtacag acactattag
     6661 tttataactc gagcaaccag atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg
     6721 atattggcaa cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttta
     6781 gggcggtatt tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca
     6841 gtctacgtcg tcgtcgaatc ccccagatat cgaactgatt tttcgccttt catacttgca
     6901 aaagcggaga atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg
     6961 atgaccatgt acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca
     7021 gacaaccccg gcatcgacgc cgaaaacgcg aatgtgcaac agttcaatgc ccaaaaatac
     7081 gttttgcagg acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa
     7141 ggtgaatgtt tacccatgct tagcggtgaa gctgcgcaaa gtgtctttga cggcgactat
     7201 gatgagatag agatccgcca ggagtggcaa gatgagaata cattacatga atgggacgag
     7261 ggggaatttc agcttgagcc accgctggat accgaggaag gacgcacagc agctgatgag
     7321 tgggatgaac gttaatcact catacgggcc atgatgaatg tcgatcggca gcagtaaggt
     7381 atcgaaaacc agggagaatg gcagatcaag gatcgtgacg taacgccagg cggagtcacg
     7441 cacgtcccat tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg
     7501 actaatgatg ctgccacagc cactcagcaa ggtcaccatt atgctcacca caattaaacg
     7561 catttgatac ctcttccgta aaaaataccg gcataacgcc ggtattttta ttggctcgtt
     7621 gtttttgccg gatgcggcgt gaacgccttg ccagacccac aaaatcttgc aaaatcaata
     7681 aattgcagga acgatgtagg cctgataagc gtagcgcatc aggcaactac gtttttattg
     7741 cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt cgtaataact
     7801 cacccatgaa gaatatctct ccggggaatt ccagacgcgg aaatgcagac gggccatcgt
     7861 caccggatcg cttagcagca ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt
     7921 cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttgctgg cgcggtgacg
     7981 cgcggtcgcc attgcggttg ccagagcgtt aaatgacggg ttaaacaccg cgtgcataaa
     8041 accatcatca agggagcgtt gacgattcat ctcaaggaac cgatcggtat caaccagcac
     8101 ctgcggcggt gaatactctt ccgggatcag gaacagtttc cagcgtttgg ttcgcagacc
     8161 aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg agaagacaat
     8221 cggtgccagc cagaacagaa aacgcagatc cagccacgcc atcccaacag cccacactaa
     8281 ccctaacagc agctgtgagc cgtggcgttt gaacgcttcc ccccaggaag tggagtcatc
     8341 atcacgctgc ggcgaattcc acactacttc ccagccaagg aacgcgctga cgacgaagac
     8401 cgtatggaac agcatgcgta ccggagccag cagcacggaa aacagcactt ccagcagcaa
     8461 cgataatgta acgcgccaga agccgccgta ttctttcgtt cctttgcacc agataagcaa
     8521 aatgctcaat agcttcggca ggaacaacag caccatggtc gaagcaaaaa gtgcaatcgc
     8581 cagctcagga cgccactgcg gccataccgg gaacaactgc cgtggttgca ggaagtattg
     8641 cggttcggtc aacgcatgta ctacctgcaa tgcagtagag agcgcgagga acataaacca
     8701 cagcggagcg gagagataag acatcacgcc cgtcaggaac accgcacggt gaaccgggtg
     8761 catacccttc accaggaaca gacggaagtt catcaggtta ccgtggcacc agcggcggtc
     8821 acgttttagc tcatcaagca agttaggcgg caattcttca taagaacccg ggagatcgta
     8881 agcaatccag accccccaac ctgcacggcg catcaacgcc gcttccacga agtcatgtga
     8941 caggattgaa ccggcaaaag aaccttcgcc cggcagcgga gccagtgcac agtgctcgat
     9001 aaacggtttc acgcggataa tcgcgttatg cccccagtag tgcgactcgc caagttgcca
     9061 gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact gctgacagcg
     9121 cgcatacagc gtatccatgc cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg
     9181 gttggcttcc atcaggcgca ccaggccgca caaacaatca ccggtcatta ccgagtcagc
     9241 atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt catcgatatt
     9301 accgctttta cgcttcacgc gacggcggcg gcggcgatag aaaatctgac cttcgccacc
     9361 gacttcagcg ataagctcca tccaggcttt ttgctctgcg acgcagatat ccgggttata
     9421 actgtcactc agaatgtaga catcaaagtg tttggcattc ccggtggctt ttactgattc
     9481 ccacgttgca cgcaagccag caaaaacacg gttcacgtct tcgttacaga taggcatgat
     9541 caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca actgttgacg cagatatact
     9601 gtatttatcg cgaccaataa gcagttgcag gaagcccatc aacgccgtcc agaacccggc
     9661 ggacacccaa cagaacagta ccgcaaagag gatcaggata ccggtttgca gcatataagg
     9721 cagaagctgc ataaaggaaa cccacacatc ctgaccaacc atatccatag gattaatcag
     9781 cgcccacccc tgataaggaa gaatggtctt catataccag gtcgcgacaa cagtttgcgc
     9841 gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg acggtacgcc acttttgctc
     9901 actctcctgc tcttctttgg tcaaacgagc cagatagcgc ggcgtcacat cgcgtccacg
     9961 caggcgatcc cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa acatcgagga
    10021 gcgttttgct tctggcatcg cctttagctg atcgcgccct tcgtcgtctt taattaactg
    10081 tccatcagca agtgaatctg gccaggcttg ttccagacgc gcctttaccg agccttgcgg
    10141 ggagtcatcc tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg
    10201 gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg atgggcattg cgtcaatgta
    10261 ctcagttgtc ttattcattg gcaggtaact ggtagctcca ggtttcactc aacgtctgat
    10321 cggcattcac cagcgcagca cgcatttcag tggttttctt ggcatctttc actttcacac
    10381 gcatcaccag acgccaacct ttggtaaccg ggttatagcg caccgtgctt tcaactatct
    10441 caccattatc accaatgctg gtttgcgctg tgaccggggt atcctctggc agttttttca
    10501 tctcagcgcc ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg cgaatcaggt
    10561 tcgactgctt cacatccccc gttgaacgac gcgtttgttg cacccatgcg ttatctggcg
    10621 catgcagttt gtcttcatca cggctgaagg tgatggtgta tttaaagttc atctctttac
    10681 ccggctccgg cagctgatcc ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt
    10741 tagttggaat ttccaccagc tcaacgctgc ctttgcccca ctcccctttc ggagtcaccc
    10801 atgcgcttgg acgaagatcg taacgatcat cgagatcttc aaagcgggag aaatcacgac
    10861 cgcgctgcaa cagaccgaag ccttgcgggt tttccatcga gaagctgctg acagctaaat
    10921 gtttcgggtt attcaacgga cgccagatcc attcgccatt accagcatgg atagacagac
    10981 cgttagagtc gtgcaactcc ggacgatagt tatttgcagg cgacggttgg ttcggcccaa
    11041 acaggaacat actggttaac ggtgcaaccc ccagtttgcc gactttatcg cgcagataga
    11101 ttttcgactg cacatccaca accgtgtcac gtcctggcat aactacgaat ttgtaagcac
    11161 ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa acgtttatca gtcggttttg
    11221 gacgctcgat ccagaactct ttgaagcgtg gaaattcttc acccgatggc aaggcggtat
    11281 caattgccag gccgcgtgca gaaaggccat aaacctgacc tgcaccaatc acgcggaaat
    11341 agctggcccc gagcatgctg acgatttcat cgtttttatc tttgctgttg atcgggtaaa
    11401 gcactttgaa accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc
    11461 cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac tgcggtggca gtcacttcat
    11521 ttattttgac cggggtatcg aagtacatac cctgatggta gaactcgagt ttgaatgggg
    11581 tcttcagatt gttccagtac gctttgtcat gattaaactg gatctgctga tagtccgcgt
    11641 atttcatatc gcggaaaacg gagggcaagt tgcttttggg cgcctcatag cctttcccgg
    11701 ctaaggattg agcttgcttt gcgacatcat caatactgaa agcccagctt gaagatgtat
    11761 acagggttaa cattactgca gcactcaacc aacgcatttt catcatttgt agtttatgtt
    11821 tcataattag taagcacttc cccctttgtg tgcttatatc gatccgatct attttaatgg
    11881 ataacctgcc attccgacaa cagaatcccg gtatcgttca gcacactggc tcatggatta
    11941 agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga agcatcgact
    12001 tcgtgtaggg tttgccagca aatttattta tcaaccgtct tacagacgct tccattctgg
    12061 ttagtggtag cgatgatgct atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg
    12121 tcggtgtcac tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc
    12181 tgagatcagt taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc
    12241 catccgcgcc tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag
    12301 ccatacatgg catgtgaata gcgccgagcc gtcattgtgg ctgacccttt ttaatgactt
    12361 catccactcg ttccgcatgc aggtgttttt cgttatatcc gggtactttt cctacatgct
    12421 ttttttacgc tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg taggtatccc
    12481 gatgttaaca gccatcccct tactgacatt gccgcaattt attatgctgc aatacgtcaa
    12541 aggcaaagcg gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg
    12601 ggaattaata tcacacctgt ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt
    12661 atg
//
LOCUS       DAPQZD010000142        12584 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPQZD010000142 DAPQZD010000000
VERSION     DAPQZD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.144"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            50..1102
                     /locus_tag="RO454_003812"
     CDS             50..1102
                     /locus_tag="RO454_003812"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDY0935575.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1178..2611
                     /locus_tag="RO454_003813"
     CDS             1178..2611
                     /locus_tag="RO454_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDY0935576.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2794..5055
                     /locus_tag="RO454_003814"
     CDS             2794..5055
                     /locus_tag="RO454_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDY0935577.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5196..6479)
                     /locus_tag="RO454_003815"
     CDS             complement(5196..6479)
                     /locus_tag="RO454_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDY0935578.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6631..7107)
                     /locus_tag="RO454_003816"
     CDS             complement(6631..7107)
                     /locus_tag="RO454_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDY0935579.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(7166..8455)
                     /gene="bioA"
                     /locus_tag="RO454_003817"
     CDS             complement(7166..8455)
                     /gene="bioA"
                     /locus_tag="RO454_003817"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDY0935580.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            8542..9582
                     /gene="bioB"
                     /locus_tag="RO454_003818"
     CDS             8542..9582
                     /gene="bioB"
                     /locus_tag="RO454_003818"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDY0935581.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            9579..10733
                     /gene="bioF"
                     /locus_tag="RO454_003819"
     CDS             9579..10733
                     /gene="bioF"
                     /locus_tag="RO454_003819"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDY0935582.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            10720..11475
                     /gene="bioC"
                     /locus_tag="RO454_003820"
     CDS             10720..11475
                     /gene="bioC"
                     /locus_tag="RO454_003820"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDY0935583.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            11468..12145
                     /gene="bioD"
                     /locus_tag="RO454_003821"
     CDS             11468..12145
                     /gene="bioD"
                     /locus_tag="RO454_003821"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDY0935584.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         3012 a         3165 c         3419 g         2988 t
ORIGIN      
        1 aatgtaacca tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat
       61 accctgcgtg atgatgcgag gtggaacctc gaggggcgcg ttcctgttag cggaacatct
      121 acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga
      181 tctggaaatt gacggtattg gcggcggtaa tccgctgacc agtaaagtcg ccattattag
      241 ccgctccagc gatccgcgtg cagatgtcga ttatctgttt gctcaagtta tcgtccatga
      301 gcaacgtgtc gataccacgc ctaactgcgg caatatgctg tctggcgttg gggcattcgc
      361 cattgaaaat ggtttgattg cagcgacttc gccagttacc cgcgtacgta tccgcaacgt
      421 caatacgggt acgttcatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga
      481 gggtagcgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct
      541 gaatgccgcc ggaacgaaaa ccggaaaagt cttcccgacg gataatcaga ttgattattt
      601 tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gttgtcatta ttcctgctga
      661 atatctggga aaaacaggct atgaattacc ggcagagctg gatgccgaca aagcattatt
      721 agcccgcatt gaatctatcc gtctacaagc gggtaaagca atgggtttag gcgatgtcag
      781 taatatggtt atccctaaac ctgtacttat ttctccagcg cagaaaggcg gggcaattaa
      841 tgtgcgttat tttatgccgc attcttgcca tcgcgctttg gcgataaccg gtgctattgc
      901 tatttccagt agttgtgcat tggaaggtac cgtcacccga caaatcgtcc cttctgtagg
      961 atacggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga
     1021 aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acgaccagaa aaatattttc
     1081 cggtgaagtt tatcttccct gaaaaatttt gttgtcgtga taatgacaat caataaagga
     1141 attctgtatg agtcatacag aaagaacagg atattaaatg aataagaaat cgttatggaa
     1201 gctaattctg atattagcaa tcccatgtat tattggcttt atgccagctc cggcaggatt
     1261 aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg ggctggttat
     1321 caagcctttc ccggaacctg tcgtactgtt aattgccgtc gccgcatcga tggtagtggt
     1381 gggtaactta tcaggtgggg aatttaaaac caccgctgta ttaagcggtt actcttccgg
     1441 taccacctgg ctggtgtttt ctgcgtttac tttaagcgcc gcgtttgtaa ccacaggctt
     1501 aggtaaacgt attgcctatc tgctgattgg taaaattggt agcactaccc tgggtctggg
     1561 ttacgttacg gtatttctcg atctggtact ggctccggca acaccgtcta acaccgcgcg
     1621 tgcgggcggc atcgtgttac cgatcatcaa cagcgtggca gtggctttgg gatcagaacc
     1681 ggaaaaaagt ccgcgtcgtg ttggacatta cctgatgatg tccatttaca tggtcaccaa
     1741 aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat
     1801 caacgacatt ctgcacctgc aaattagctg gggtggatgg gcgttagccg ccggattgcc
     1861 gggcatcatt atgctgctgg tcaccccgct ggtgatttac accatgtatc caccagaaat
     1921 taagaaggtg gataacaaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa
     1981 aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctgctgggct ggattttcag
     2041 taagtctctg ggggttgatg aatccaccgt ggcaatcgtt gttatggcaa ctatgctgct
     2101 gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat
     2161 ctggtacggc ggtattatcg gcttaagctc cttattatcg aaagttaaat tcttcgaatg
     2221 gttagctgaa gtctttaaaa ataacctggc atttgatggt cacgggaacg ttgctttctt
     2281 cgttattatt ttcctcagca tcatcgtgcg ttatttcttc gcttctggta gtgcctatat
     2341 cgttgccatg ttaccggtat ttgccatgct ggcgaatgtc tccggcgcac cgttaatgtt
     2401 aaccgcgctg gcactgttgt tctctaactc ctatggcggc atggttactc actatggcgg
     2461 cgcggcaggt ccggtcatct ttggcgtggg ttacaacgat attaaatcct ggtggttggt
     2521 cggtgcggta ctgacgatat taaccttcct ggtgcatatc accctcggcg tgtggtggtg
     2581 gaatatgctg atcggctgga acatgctgta aatatactcg tcatacttca agttgcatgt
     2641 gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagtgactgg ggattcactc
     2701 gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat ccatttatta tctttctgcg
     2761 cactttccgg tgcgcagata tctggagcat ttgatgatca agttatctga aaaaggcgtg
     2821 tttctcgcca gtaataacga aataattgcc gaagaacatt tcaccggcga aattaaaaaa
     2881 gaagaagcca aaaaaggcac tattgcctgg tctattctct cttctcataa tacgtccgga
     2941 aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt
     3001 ggtattgtac agaccgctaa agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg
     3061 accaactgcc ataactcact ctgcgccgtc ggcggcacca ttaacggtga tgaccatgtt
     3121 tttggtttat cggcggccca gcgttatggc ggtatttttg tgccaccgca tattgcggtc
     3181 atccatcaat atatgcgtga aatgatggct ggcggcggca aaatgatcct cggatcagac
     3241 agccacaccc gttacggcgc attagggaca atggcagtcg gtgagggcgg cggcgagctg
     3301 gtaaaacaac tgcttaatga cacctgggat atcgactatc cgggcgtggt tgcggtgcat
     3361 ctgaccggaa aacctgcgcc gtatgtgggg ccgcaggatg tggcgctggc tatcatcggt
     3421 gccgtgttca aaaacggtta cgtcaaaaac aaagtgatgg agttcgtagg tcccggtgtt
     3481 gcagcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccactga aacgacctgt
     3541 ttaagttctg tctggcaaac cgatgaagaa gtccataact ggctggcgct gcacggtcgc
     3601 ggccaggatt actgccaact taaccctcaa ccaatggcgt actacgatgg ctgcatcagc
     3661 gttgatttaa gcgccatcaa accaatgatt gcgctgccat tccacccgag caacgtgtat
     3721 gaaatcgaca cgctgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc
     3781 gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc
     3841 ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactatga aaacgtcatc
     3901 gcggcggcga atgcgctgcg cggtcaatcc tgcggcaatg acaccttctc gttagcggtt
     3961 tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt cgcagatttg
     4021 attggcgcag gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg cgcgggcgat
     4081 acgccaatca acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa
     4141 ggctctaagc cagctaatgg gcagatgtca gcggtggcgt tgatggacgc tcgttctatc
     4201 gctgcgactg cggcaaacgg tggctattta acctctgcca gcgaactcga ttgctgggac
     4261 aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa atcgcgtcta tcaaggcttt
     4321 gtgaaagggg cgacccagca accgctgatt tacggaccga acattaaaga ctggccggaa
     4381 ttgggtgcgc tgactgacaa tatcgtcctc aaagtgtgct cgaagatcct cgacgaagtg
     4441 accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt
     4501 ggtctggcgg agtttaccct gtcacgccgc gatccaggtt atgttggcag aagtaaagcg
     4561 actgctgagc tggaaaatca gcgtctggcg gggaatgtca gcgagatggc agaggtgttt
     4621 gcgcgcatta agcagattgc tggtcaggaa catgttgatc cgctgcaaac tgaaattggc
     4681 agcatggtct atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagttgt
     4741 cagcgtgtga ttggcggtct ggcgaatatt gccgaggagt acgcgactaa acgctatcgt
     4801 tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagcgcc aacctttgaa
     4861 gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc tggaacgacg
     4921 tttaaaggtt atgtgatcca tgaagatgca ccggtaacgg aaatcacgct ctatatggaa
     4981 agtctgactg ccgaagaacg cgagattatc aaggcgggta gtttgattaa cttcaataaa
     5041 aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc atccggcact tattgtcgga
     5101 tgcgatgctg gcgcatctta tccgaccgac aaatcgtatc gaactgtagg ccggataagg
     5161 cgtttacgcc gcatccggca catagttaac agctcctact tcttcgcctc tgcaaccact
     5221 ttgctaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
     5281 ttgcgttgta cttcgtcgtt atcatcaacg ctgccggtgt tacccgcaaa cgggcgatta
     5341 gagatcaccg catcagccca cggtttggct gtgttaaaac cttcgttgat ggcgctatca
     5401 cggatcacta cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc
     5461 acgccatcac cggaagcatt gaaacggctg tttacggcga ggaaaccgta gtaaatgttg
     5521 gacagcgtag ccggtgcaaa cacatacgct tcttgctggg tacgggagtt caccacgcgg
     5581 aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
     5641 atatagctgt tggtcaccag cgtacgcggc tggcgattcg tttccagacg gttctgcaca
     5701 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttgacgtt attgatctgc
     5761 actttgtcgc catcagtacg cagtgccacc gccggatggt tacccgcatc tacgctatcg
     5821 cccagcgtgt tttcgatggt caggttttgc agttgcaggc cattgttttg tgaccagaag
     5881 accgcagagc agagaacacc gatactgtcg ctgcgtttac tctggcagct atcgtacata
     5941 taccacgccg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
     6001 gcagggctca tgccaccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
     6061 gtaccgtaca gagtaattcc acccggagcg gcagggacat acaccgttcc ctgatactca
     6121 ccaggcatca cggcaatata ctggcgcttg ttggtacgct tgataattgc cgcatctacc
     6181 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
     6241 ggttgcgcag gcagggtaat cggggaagga ttccacgctg ccgcacctgg tgtcagggat
     6301 gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa
     6361 gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gcggtgttga gctacaggcg
     6421 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
     6481 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
     6541 cttgagcgaa acgggaaggt aaaaagacaa aaagttgttt ttaatacctt taagtgatac
     6601 cagatggcat tgcgccatct ggcagagtga ttaactaaac atcgcagtaa tcgaggcact
     6661 cgccagagag tgaaaatgaa cgttaaaccc gaccatcgcg ccgctggcac cttcatcgac
     6721 atcaatacgt tctacatcca gcgcgtgaac ggtaaaaatg tagcgatggg tttcgccttt
     6781 cggcggcgct gcgccatcgt atccggtttt accaaagtcg gtacgcgtct gcaaaacgcc
     6841 gtctggcatt gctaccagac cagatccaaa cccttgcggt aatacgcggg tatccgcggg
     6901 taaattaaca actacccagt gccaccagcc ggagccagtt ggcgcatccg ggtcgtagca
     6961 ggtgacaaca aaacttttcg ttcccacagg aacatcatcc cacgccagat gcggtgaaat
     7021 attatcgcca tcgtaaccca tgccgttaaa gacatgacga tgcggcagct tatcgccatc
     7081 gcgtaaatca ttactgatga gtttcatgag ccctcctttc ttgtttgcag aaagtgtagc
     7141 cagaaaccct cacgctgact tcccgttatt ggcaaaaaaa tgtttcatcc tgcaccgcac
     7201 gattaaccgc tgcggtcaga cgctgcaact gttgcgggag aataatatag ggcggcatca
     7261 ggtaaatcag tttgccaaaa ggccggatcc agacaccctg ttcgacaaag aatttttgca
     7321 gcgctgccat attcaccgga cgagaggttt cgaccacgcc gattgccccc agtacgcgca
     7381 catcggcaac catttcggcg tcacaggcgg gggcaagttg ctcgcgtagc tgcgcttcaa
     7441 tagccgccac ctgatgctgc cagtcgccag attcgagaat cgccaggctg gcgtttgctg
     7501 ctgcgcaggc cagcggattg cccataaaag ttggcccatg cataaagcag ccggcttcgc
     7561 cgttactgat ggtttccgca acctcgcgcg tggtgagcgt ggcggaaagg gtcattgtgc
     7621 cgccggttaa ggctttaccg agacacaaaa tatccggcgc gatttctgca tgttcacagg
     7681 caaacagttt gccggtacga ccaaatccgg tggcgatctc gtcggcaatc agcaagatac
     7741 cttcgcgatc gcacattttg cggattcgtt ttaaccattc cggatggtac atgcgcatcc
     7801 cgcctgcgcc ctggacaatc ggttcaatga tcaccgccgc gatttcatga cgatgcgccg
     7861 ccatcaggcg ggcaaagccc accatatcgc gctcatccca ttcgccatcc atgcggcttt
     7921 gcggggcggg agcaaacagg ttttctggca gatagccttt ccacaggttg tgcattgagt
     7981 tgtccggatc gcacaccgac atcgcgccaa aggtatcgcc atgataacca ttgcggaagg
     8041 ttagaaaacg ctggcgcgct tcgcctttgg cctgccagta ctgcaacgcc attttcatcg
     8101 ccacttctac cgctacggag ccggagtccg cgagaaaaac gcactccagt ggttgcggcg
     8161 tcattgccac caatttgcgg cacagctcaa tggcgggcgc atgggtgata ccgccaaaca
     8221 tcacatgcga catagcatca atttgcgact tcatcgccgc attaagctgc gggtgattgt
     8281 aaccgtggat cgccgcccac caggacgaca taccgtcaac caggcgtctg ccgtcagaca
     8341 aaattagctc gcaaccttcg gcgcttgcca ccggataaac cggcagcggg gaggtcatgg
     8401 atgtgtatgg gtgccagata tggcgttggt caaaggcaag atcgtccgtt gtcataatcg
     8461 acttgtaaac caaattgaaa agatttaggt ttacgagtct acaccgaatt aacaacaaaa
     8521 aacacgtttt ggagaagccc catggctcac cgcccacgct ggacattgtc gcaagtcaca
     8581 gaattatttg aaaaaccgtt gctggatctg ctgtttgaag cgcagcaggt gcatcgtcag
     8641 catttcgatc ctcgtcaggt gcaggtcagc actttgctgt cgattaagac cggagcttgt
     8701 ccggaagatt gcaaatattg cccgcaaagc tcgcgctaca aaaccgggct ggaagccgag
     8761 cggttgatgg aagttgaaca ggtgctggag tcggcgcgca aagcgaaagc ggcgggatcg
     8821 acgcgtttct gtatgggcgc ggcgtggaag aatccccacg aacgcgatat gccgtacctg
     8881 gaacaaatgg tgcagggggt aaaagcgatg gggctggagg cgtgtatgac gctgggcacg
     8941 ttgagtgaat ctcaggcgca gcgcctggcg aatgccgggc tggattacta caaccacaac
     9001 ctcgacacct cgccggagtt ttacggcaat atcatcacca cgcgtaccta tcaggaacgc
     9061 ctcgatacgc tggaaaaagt gcgcgacgcc gggatcaaag tctgttcagg cggcattgtg
     9121 ggcttaggcg aaacagtaaa agatcgcgcc ggattattgc tgcaactggc aaacctgcca
     9181 acgccgccgg aaagcgtacc aatcaacatg ctggtgaagg tgaaaggcac gccgctggcg
     9241 gataacgatg atgtcgatgc ctttgatttt attcgcacca ttgcagttgc gcggatcatg
     9301 atgccgacct cttacgtacg cctttctgcc ggacgcgagc agatgaacga acagactcag
     9361 gcgatgtgct ttatggctgg cgcaaactcg attttctacg gttgcaaact gctgaccacg
     9421 ccgaatccgg aagaagataa agacctgcaa ctgttccgca aactggggct aaatccgcag
     9481 caaactgccg tgctggcggg cgataacgaa caacagcagc gtctggagca ggcgctgatg
     9541 accccggaca ctgacgaata ttacaacgcg gcagcactat gagctggcag gagaaaatca
     9601 acgcggcgct cgatgcgcgg cgtgctgccg atgccctgcg tcgccgttat ccggtggcgc
     9661 aaggagccgg acgctggctg gtggtggatg atcgccagta tctgaacttt tccagtaacg
     9721 attatttagg tttaagccat catccgcaaa ttatccgtgc ctggaagcag ggtgcggagc
     9781 aatttggcat cggtagcggc ggctccggtc acgtcagcgg ttatagcgtg gcgcatcagg
     9841 cgctggaaga agaactggcc gagtggctgg gctattcgcg ggcactgctg tttatctctg
     9901 gttttgctgc taatcaggca gtcattgccg cgatgatggc gaaagaggac cgtattgttg
     9961 ccgaccggct tagccatgcc tcattgctgg aggctgcaag tttaagcccg tcgccgcttc
    10021 gccgttttgc tcataacgat gtcactcatc tggcgcgact gcttgcttcc ccctgtccgg
    10081 ggcagcaact ggtagtgaca gaaggcgtgt tcagcatgga cggcgatagt gcgccactgg
    10141 aggaaatcca gcaggtaacg caacagcacg atggctggtt gatggtcgat gacgcccacg
    10201 gcacgggcgt tatcggggag caggggcgtg gcagctgctg gctgcaaaag gtaaaaccag
    10261 aattgctggt ggtgacgttt ggcaaaggat ttggcgtcag cggggcagcg gtgctttgct
    10321 ccaatacggt ggcggattat ctgctgcaat tcgctcgcca tcttatctac agcaccagta
    10381 tgccgcccgc tcaggcgcag gcattacgtg cgtcgctggc agtcattcgc agtgatgagg
    10441 gggatgcacg gcgcgaaaaa ctggcggcac tcataacgcg ttttcgtgcc ggagtgcagg
    10501 atttgccgtt tacgcttgct gattcatgga gcgccatcca gccattgatc gtcggtgata
    10561 acagccgtgc gttacaactg gcagaaaaac tgcgccagca aggctgttgg gtcacggcaa
    10621 ttcgcccgcc aaccgtaccc gctggtactg cgcgactgcg cttaacgcta accgcagcgc
    10681 atgaaatgca ggatatcgac cgtctgctgg aggtgctgca tggcaacggt taataaacaa
    10741 gccattgctg cggcatttgg acgggcagcc gcacactatg agcaacacgc cgatctacag
    10801 cgccagagtg ctgacgcctt actggcaatg ctcccacagc gtaaatacac ccgcgtactg
    10861 gatgcgggtt gtggaccagg ctggatgagc cgccgctggc gagagcgtca cgcgcaggtg
    10921 actgcgttag atctctctcc gccaatgctt gttcaggcac gccagaagga tgccgcagac
    10981 cattatctgg cgggagatat cgaatccctg ccgttagcga ctgcgacgtt cgatcttgca
    11041 tggagcaatc tcgcggtgca gtggtgcggt aatttatcca cggcactccg cgagctgtat
    11101 cgggtggtgc gccccggagg cgtggtcgcg tttaccacgc tggtgcaggg atcgttaccc
    11161 gaactgcatc aggcgtggca ggcggtggac gagcgtccgc atgctaatcg ctttttaccg
    11221 ccagatgaaa tcgaacagtc gctaaacgcc gtgcattatc aacattatat tcagcccatc
    11281 acgctgtggt ttgatgatgc gctcagtgcc atgcgttcgc tgaaaggcat cggtgccacg
    11341 catcttcatg aagggcgtga cccgcgaata ttaacgcgtt cgcagttgca gcgattgcaa
    11401 ctggcctggc cgcaacaaca ggggcgatat cctctgacgt atcatctttt tttgggagtg
    11461 attgctcgtg agtaaatgtt attttgtcac cggaacggat accgaagtgg ggaaaactgt
    11521 cgccagctgc gcgcttttac aagccgcaaa ggcagcaggc taccggacgg caggttacaa
    11581 accggtggcc tctggcagcg aaaagacccc gaaaggctta cgtaatagtg acgcgctggc
    11641 gttacagcgc aacagtagcc tgcagctgga ttacgcaacg gtaaatcctt acaccttcgc
    11701 agaacccacc tcgccgcata tcatcagcgc gcaagagggc aggccgatag aagcattggt
    11761 aatgagcgcc ggattacgcg cgcttgaaca acaggctgac tgggtgttag tggaaggtgc
    11821 tggcggctgg tttacgccgc tttctgacac tttcactttt gcggattggg taacacagga
    11881 acaactgcca gtgatactgg ttgtgggcgt gaaactcggc tgtattaatc acgcgatgtt
    11941 gactgcgcag gcaatactac acgccggact gactctggcg gggtgggtgg cgaacgatgt
    12001 cacgcctccg ggaaaacgtc acgctgaata tatgaccacg ctcacccgca tgattcccgc
    12061 gccgctgctg ggcgaaattc cctggcttgc agaaaatcca gaaaatgcgg caaccggaaa
    12121 gtacatcaat cttgccttaa tgtagtcatc ctgtaattgg ttaaattgcg agcgagatcg
    12181 tgtctccgat tgactgcaat ttaaccaatt aaattctaaa aaaatcacga aaaaaatttt
    12241 acctccgcat catgcggtga atgtgggaat tgcccaggcg gcgggggata ggggctgcag
    12301 acagttatcc actattcctg tggataacca tgtgtattag agttagaaaa cacgaggcaa
    12361 gcgagagaat acgcggcttg cacgcgaatt ggcgttaaag acagctcaaa gaaatatctt
    12421 ttatttttta acaggttaga taattgcaat ggcagtcact gaacaggcat ctcttgccat
    12481 aaaactgtca tcaatcatct tgacaaatgt taaaaaagcc gttgctttgg ggataacccg
    12541 gtaaggccgg agttttatct cgccgcagag taaatcttgt tcat
//
LOCUS       DAPQZD010000143        12501 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPQZD010000143 DAPQZD010000000
VERSION     DAPQZD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.145"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            8..387
                     /locus_tag="RO454_003822"
                     /pseudo
     CDS             8..387
                     /locus_tag="RO454_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            577..1143
                     /gene="slp"
                     /locus_tag="RO454_003823"
     CDS             577..1143
                     /gene="slp"
                     /locus_tag="RO454_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDY0935585.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1296..1826
                     /locus_tag="RO454_003824"
     CDS             1296..1826
                     /locus_tag="RO454_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0935586.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(1883..2911)
                     /gene="chuS"
                     /locus_tag="RO454_003825"
     CDS             complement(1883..2911)
                     /gene="chuS"
                     /locus_tag="RO454_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDY0935587.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2960..4942)
                     /gene="chuA"
                     /locus_tag="RO454_003826"
                     /gene_synonym="shuA"
     CDS             complement(2960..4942)
                     /gene="chuA"
                     /locus_tag="RO454_003826"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDY0935588.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            5626..6540
                     /gene="chuT"
                     /locus_tag="RO454_003827"
     CDS             5626..6540
                     /gene="chuT"
                     /locus_tag="RO454_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HDY0935589.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            6560..7897
                     /locus_tag="RO454_003828"
     CDS             6560..7897
                     /locus_tag="RO454_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDY0935590.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLVQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDVCAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            7910..8404
                     /gene="hutX"
                     /locus_tag="RO454_003829"
     CDS             7910..8404
                     /gene="hutX"
                     /locus_tag="RO454_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDY0935591.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            8404..9027
                     /gene="chuY"
                     /locus_tag="RO454_003830"
     CDS             8404..9027
                     /gene="chuY"
                     /locus_tag="RO454_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDY0935592.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            9112..10068
                     /gene="chuU"
                     /locus_tag="RO454_003831"
     CDS             9112..10068
                     /gene="chuU"
                     /locus_tag="RO454_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDY0935593.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            10065..10835
                     /locus_tag="RO454_003832"
     CDS             10065..10835
                     /locus_tag="RO454_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDY0935594.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(10887..11534)
                     /locus_tag="RO454_003833"
     CDS             complement(10887..11534)
                     /locus_tag="RO454_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDY0935595.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11598..11924)
                     /gene="hdeB"
                     /locus_tag="RO454_003834"
     CDS             complement(11598..11924)
                     /gene="hdeB"
                     /locus_tag="RO454_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDY0935596.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(12040..12372)
                     /gene="hdeA"
                     /locus_tag="RO454_003835"
     CDS             complement(12040..12372)
                     /gene="hdeA"
                     /locus_tag="RO454_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDY0935597.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         3298 a         3076 c         2877 g         3250 t
ORIGIN      
        1 gaatgttatg tcaattgact ttaccccagg tgtaataaat acatataacg gtgatattta
       61 taactgcaca acaaataccg ataatgttaa aacgccagac acaccgaagt ggccttgtga
      121 taaccgggaa gaacaacaac ccattaattc cccattctct ggagaaggat acacctctaa
      181 acaatttgat ttagcgcaac aacaacgcca acaaattaat gcgtgccata cgaacaccac
      241 atatacaaat gcagactact caaagttgag gctcaacttg taagtcttat taataacatt
      301 gaaacgataa gctcgacacc acttccgcaa aagacacaat caatacttaa tcagataaat
      361 aacattagac acgaaaagat taaaaacctc ctaatataaa ttatggaaca atggatactc
      421 atgccacgcc caaaaattaa ctgagctcaa ctaaacagaa gagatataaa catcagacag
      481 gtttacgtta ctatcaggca tatcgcctca gaatcagatg aaaactataa agaaatatct
      541 attatggttt taatatttgt tgataaggat agtaacatga acatgacaaa aggtgcactc
      601 atcctcagcc tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa tatcaaaggc
      661 aataaccaac ctgatattca aaaaagtttt gttgctgttc ataaccagcc ggggttatat
      721 gttggtcaac aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg caaaacggat
      781 acgttgttag aaatcgctgt attaccgttg gatagctatg cgaagcctga tattgaagcc
      841 aactatcagg gccgactgct cgccagacaa agcggcttcc ttgatccagt gaactatcgt
      901 aatcactttg ttaccatcct cggtaccatt cagggtgaac aacctggctt tatcaataaa
      961 gtcccgtata acttcctcga agtgaatatg cagggcatcc aggtgtggca tttgagagaa
     1021 gtggttaata ccacctataa cctgtgggat tacggctatg gtgcattctg gccggaaccg
     1081 ggctgggggg ctccttacta caccaatgcg gtgagtcagg taacacctga gctggtcaaa
     1141 taacaccacc gaaagatgca gatgctttct cagcatctgc atcatgcatt acatcaaatt
     1201 aatacacagt aagctaacta ttattataag ccctgtcctg ttaattacct ttggcaaact
     1261 gattataaag ttaatgtccg taccaggagt cggttatgtt tcttataatt accagggata
     1321 cgatgttttt caccgcgatg aaaaacattc tgagtaaagg taatgtcgtt catatacaga
     1381 acgaagaaga gatcgacgta atgttgcatc agaatgcctt cgtcattatt gatacattaa
     1441 tgaataatgt atttcattct aattttctca ctcaaattga acgattaaaa cctgtccatg
     1501 taattatttt ctcccccttt aatattaaac gctgcctggg gaaagtaccg gtgacctttg
     1561 ttccgcgaac tatcgccatc atcgactttg tcgcactcat caatggcagt tactgctctg
     1621 tgcctgaagc ggatgtgtcc ctctcgcgca agcagcatca ggttctgagc tgtattgcga
     1681 atcaaatgac aacggaagat attctggaga aactgaaaat atcgctaaaa acgttctact
     1741 gccataaaca caatatcatg atgatcctca atcttaagcg gatcaatgag ctggtacgcc
     1801 atcagcatat taattatctg gtgtgaacga ttgatcaata taaagaggcc cagcaacagc
     1861 cagacctccc gttaattata cgttatgcag taacgccttc cggtatcaac gaagcaattt
     1921 gcttacgcca ttgcgcttgc tcctgttcac cttctgtacg ttgaccataa agttgcgcta
     1981 tctgcgtacc atcatgggca aacaattcca gactggttac gtgaccatcg ctggtcggtt
     2041 tacgggtaac ccaggcttca gcaatgctct cttctaatag atgaagagta aacgtcgggt
     2101 tgaaaatatt cagccaacct ttcattggca ccactttttc taccacaccg gtgaaaatct
     2161 gtacgcagcc acggttgcca acaaacacca tgatttcatt gccatcctgc tgtgcagatt
     2221 caagaatttg cgccaacgca ctgttggata ctttgcaggc caaatcgtct gccaccagat
     2281 tgaacgcctg ttggcgcgtc agattgtgac gcttgagcaa cgtaaaaaac tgatgaacgt
     2341 cggtcatcgc ccgccactct tgctcgacca cactggcatc ggctcgcgtt tgaacaactg
     2401 gcgcatcaac ggcttttaac tcaagcggcg tattctcatc ggtgataaac cgtgccagaa
     2461 gctcactcca tgccgccata tcggtattgt cggtggcata aacttttagt aatgcatcgc
     2521 cctgatgatc aaagaactga atactctggc gttcgccacg ggccgtgttt tctttgatgt
     2581 ggaaaacact ggcccattga ttgagaaaca gacgtaaatc cagcgcgcgc ggattgagga
     2641 tcaatccggc atgtccgttc aaatgctggt ttgtgaacgc accaacttgc tcatggactg
     2701 catattcatt acggcaaata catttggttt cgccaacact ttcgagcgcc gccagaattt
     2761 cacgaatatc gccgcgcatc cgccacgcgt cgtgcgtgac gcgcgcaaat gccagttctg
     2821 cttcgctgat attcattaaa cctgcgatgt cacgcgcgta ctttccggga ttttgttctt
     2881 ttaactcaag ccagcgtgtg tagtggttca ttgtctctct tccttccaga taaatgcccc
     2941 gaaatatcgg ggcagatgat taccattgat aactcacgaa aatttttccg ttacgaccat
     3001 cctgtgggat gccttgcggc gaccagtact ctttatcgaa ggcgttgccc aataccagag
     3061 tagtggtcat gcctttgagc gcctgttgtc cttgataact gacgtagaaa tcattcacac
     3121 catagccagg ttgtttgctg tagctgctgc tgatatgtgt tgagcgatcg gcaaacgtac
     3181 cgacccaacc aacagagaag ccactgtgag cgatcggaat attcagggta ctggtaacgg
     3241 tatccgggtt aatgctggag atatattccc cggtatcggt gtctttgccg cgggtacggt
     3301 tataggccac atcaaggcta aacagatcag tggtatattt cgtcatcaca tcccagcccc
     3361 agattttggc gttcgggacg ttatacgaca tagttgtcgc cgccgcgaaa tcgacggtcg
     3421 tggagatata atctttcgct ttggtatcaa agtagctggc tttaaattcc agagcatcat
     3481 tggacaacat caggtcatca aaacgtagcc caaaaccgta ctcctgagtt tcgttagttt
     3541 ccggacgtaa gttcgggttt ggcacccaat agttggtata gaagcgacca atcgagaagt
     3601 gtttagaatc attatacatt tcgcccatcg tcggggcgcg gaatgcctga gcatatgagc
     3661 caaataacat cagccagttg gtcgggttga tagtcatccc cgcacgagat gaccatttgt
     3721 cggcatcaac atctttgtag ccgtcgctgc taccgcgata actgtcatag cgggttccgc
     3781 caagcagggt aatcggcaga tcgcgtaagg tgatctcatc ttgtagccag ccagagctaa
     3841 aatcgatttt tgcttgcggg aagcccgtcg tcgcgccacc cggatgttgt tcctgacgat
     3901 aatactcacc gccatatgtc agtaagtgag aagcgaaact gtcggcaaat agagtggaac
     3961 ggttctccag ccttgctcct tttgttatct gttcacgata ctcgcctgaa ctccccgtgt
     4021 tttgcgcatt aatacggact tccgaccagt atatttttgc atctgcattt aaccagtcgt
     4081 taccctgcgg ggcgagttta taagaaagct gcgcatcgcg ttgaattgtt gaacgatcga
     4141 ccatcgggtt gctgctatca gaagcttcaa cagtctgcgg attttttggt tcacgcgcgt
     4201 cgttgttgta gtaacgcact aaaccgctca gagactgggc tgaatcaatt tgccaggtcc
     4261 ctttcgccag catgttatta atggattcgt cattcggcgc ggtttcacca ttgctctggc
     4321 gtaaatcacc ccgatcgcga ctggaccagg ccacaatacc atccagattt tcagttcgcc
     4381 caaacgcgct cgcgcctaat cccaggctat ggtcccccgt gccgccagta ccaaagacac
     4441 gaaaaccact gctttgtcct tcctgcaata aatcttttgc atcgaccgta tcgtaggaga
     4501 tcactccacc cagcgcgcca ctgccataca gtaatgctga aggtccgcga acaatctcaa
     4561 cacgcttgat cagcgccgga tcgagaaaag tgccattcag gtgtccggta tccgttccct
     4621 ggcgaacacc atcgacaaga accagcacgc cgcgatgatc atagccacgc atatttacat
     4681 cctgaccgtt ggttcgtccg gtaccatcaa gagtaattcc aggaacatga cgcagcaaat
     4741 cagtggctga agtagcagtt tgattttcag gagcggaagt gtcgataacg ctgaccatca
     4801 taggcgcttc gaaggaacta cgtgcattcc ccgttgccgt aacggtcatg gtttcagtag
     4861 caaaagcaaa cgttggcaag gtggcagaaa cagccaaagc caacaaactc aaacgcaacg
     4921 aagtaaattg cggacgtgac atagcgattc tccatgagga aaataaaaaa tgctggctgc
     4981 ctgggttaat accggggtgg ctggcgaaaa ataaatttat ttacaattca aatgattaga
     5041 tatcaccacg ggaagctatt agcgtatata tcccgtaaaa ttgccgaata tttctgataa
     5101 ttcagcaaaa gcacgccctc tttgtcaggc aaagtagtac gccacgtctg aagtgaagag
     5161 tgctgtgcgt tttcccttac aaaagatatg attttgttat ccatgatgtc atattttgtt
     5221 aaactcaaac gtgataatga gaattattat cgatcaattt tttatgatca agcttttttt
     5281 aaccgccatt aacacaataa acagggggtt atgaaatcta tccagcatcc agaaaaccat
     5341 cctgtgttta tggctatccc cccacaacct catcacaaaa cgttgtcgtt aacagtgata
     5401 attttctctt ctctttgctc tgcaagccgc gatatctccg ggttctcagc tttttcccgc
     5461 gctgaaattt aatcggtaac aagaacatta gctgacgaag catcttgata aaaatcaaaa
     5521 ataaatacgc ttgtaacgcc gtgcgtattg aatcgacggt tgtatttcat atataactca
     5581 aatgataatc atgatcattc tcaatttgat aagagttctc acattatgcc aaggatcatc
     5641 accaggccgt ttcttttttc cccactaacg ttatgtattt cagccgtggt ctctgccgcg
     5701 aagaccatgg taaaacgaaa aaaattgttt acggcattac tggctcttag ctggactttt
     5761 agcgtaacgg ctgccgaacg tatcgtggtc gcaggaggat cgctgacgga gctaatctac
     5821 gcgatgggcg ctggcaaacg cgtggttggt gtcgatgaaa cgacatccta tccaccagaa
     5881 accgccaaac tgccgcatat tggttactgg aaacaactca gcagcgaagg cattttgtca
     5941 cttcgcccgg atagcgtaat tacctggcag gatgcaggac cgcaaattgt gctcgaccaa
     6001 ctgcgggcgc aaaaagtcaa cgtcgtcact ctgccgcgtg taccagccac ccttgagcag
     6061 atgtacgcca acattcgcca actggcaaaa acgttacagg ttcctgaaca aggcgaggcg
     6121 ctggtgacac agatcaacca acgcctggag cgagtacagc aaaacgtggc ggccaaaaaa
     6181 gccccggtta aagccatgtt tattctctcc gcaggcggaa gtgcgccaca ggttgccggt
     6241 aaaggcagcg tcgcggacgc cattctttcg cttgccggag cagaaaacgt cgctacccac
     6301 cagcagtaca aaagctacag cgcggaatcg ctgattgcgg ctaaccctga agtgattgtc
     6361 gtcacctcac aaatggtcga tggcgatatt aatcggctac gttctattgc cggaattacc
     6421 cacactgccg catggaaaaa ccagcgcatt atcaccgttg atcaaaacct gattctggga
     6481 atgggtccgc gtattgctga tgtcgttgag tctttacacc agcagctttg gccgcaataa
     6541 atggatttaa atcagacgaa tgaacacaaa taacaccctg gatctcacgc cacattttgc
     6601 tttagacggt gatcagccct ttaaagacag gcgcgccatg atgccgtttc gtggggccat
     6661 tccggttgcc aaagagcaac tggtacagac ctggcaagag atgatcaatc aaacggtatc
     6721 accacgtaag cgactggttt acctacacat tcctttttgt gcgacacact gcacattttg
     6781 tggtttttat cagaaccgtt ttaatgaaga tgtgtgtgct cattataccg acgccctgat
     6841 tcgtgaaata gaactggaag cagacagcgt attgcatcag tcgggcccca tccacgcggt
     6901 ctatttcggt ggcggtacgc cttccgcgct ttcagcacac gatttggcca gaatcatcac
     6961 cacattacga gaaaagttgc cactggcccc tgattgtgaa atcactatcg aaggccgggt
     7021 actgaatttt gacgctgagc gaatcgatgc ttgccttgat gctggagcaa accgcttctc
     7081 aattggtatt cagtcgttta acagcaaaat ccgcaagaaa atggcccgca cctcggatgg
     7141 cccaaccgct attgcgttta tggaaagcct ggtcaaacgc gaccgtgccg cggtggtctg
     7201 tgacctgctg tttggtctgc cgggtcagga tgcgcaaacc tggggagaag atctggctat
     7261 tgcccgcgat atcggtctcg acggcgtcga tctctatgcg ctcaatgtcc tgcccaatac
     7321 accgctgggc aaagccgtgg aaaatgggcg tactaccgtg ccctctccgg cagaacgtcg
     7381 cgatctttac ctgcaagggt gtgattttat ggacgatgcc ggttggcgct gcatcagtaa
     7441 cagccactgg ggccgtacca cacgcgaacg caatctctat aacctgctga ttaaacaagg
     7501 tgccgattgt ctggcctttg gttccggagc cggcggttcg attaatggtt actcctggat
     7561 gaacgaacgc aatcttcaga cctggcatga atccgtcgca gcaggcaaaa aaccgctgat
     7621 gatgatcatg cgtaacgccg aacgtaacgc gcagtggcgt cataccttgc agtcgggcat
     7681 tgaaacggcg cgtgtaccgc tggacgaact tacgccacat gccgaaaaac tcgcgccgtt
     7741 actggcccag tggcaccaaa aaggcttaag ccgcgatgcg tcaacgtgtc tgcggttgac
     7801 caatgaaggt cgtttctggg caagcaacat tttgcagtct cttaatgaac tgattcaggt
     7861 acttaatgcg ccagcaattg cgcttgaaaa accataacag gagtgattta tgagccatgt
     7921 ctcgttacag gaatttttga aaacggagcc agacggtacg ctggaggcga tcgccgaaca
     7981 atacaacacc acgctgctgg aggtagtgaa aaatctgccc tctccgactg ttgtgtcagg
     8041 tgataaattc gatactgtct gggataccgt ctgcgaatgg ggcaacgtca ccacgttagt
     8101 acatactacc gatgtaatac tcgaatttag cggcgaactg ccttcgggtt tccatcgcca
     8161 cggctatttc aacctacgcg gaaagcacgg catgtccggg catatcaaag cagaaaactg
     8221 cacgcatatt gccttgatcg aacgtaaatt tatgggcatg gacaccgcct ctattctctt
     8281 tttcaataag gaaggcagcg caatgctgaa aatcttcctc ggtagggatg atcatcgtca
     8341 gctcctgagc gaacaagtca gcgccttcca tgctctggca gcatcgttaa aggaacacgc
     8401 ctgatgacac cgtggctact ctttggtgcg ggcggaaaag gtgtgggtgc cagaacgctt
     8461 gaactggcgc tggcggaaca acgtccggtc gttgctgtca ttcgtcatgc cgatgccgcc
     8521 acgaaactgg cgcaacaagg cgtacaggtt tttacaggcg acgcctgtga tgccagcgta
     8581 gtcgcggctg cttgccgcgc agctggcccg gatgcgctta tcatctcaac aatgggcgga
     8641 gctcaggatt atctggcgca cagaacagtg attgatgagg ccgagaaggc tggcatcagt
     8701 cgcatgattc tggtgacctc tttgggatgc ggcgatagct ggccgttttt atcagagcgg
     8761 gcaaaggctg cgtttggtca ggcggtacgg gaaaaaacgc tggcggaaag ctggttgcaa
     8821 accagccagc ttgattatgc cattctccgc cctggcggtc tgcttgatgg tgcggcaacg
     8881 ggcaaagcac aacggataca aaatcaggag tgtcatggtt ttgttaaccg tgctgatgtc
     8941 gccgcacata tccatgaact ggcaaatgcg ccagcactta accagcaggt ctacagtctg
     9001 attgaaccgg acctgaaacc ggcgtgatcc cccggcgcgg catgtccgcg ccgtaatgtc
     9061 gtaacggagt tttgcgtgct caaggattca ctctcttccg ccagggtctt tatggggcta
     9121 tcactattat tgctagctct ggtgctgttt ggtgccagtc agggagcgtt aaagatcagt
     9181 tttgatgccc tttttgatga ggaataccgc gatatctggc tcaatattcg tctaccacgg
     9241 gttttgctgg cggtgctggt aggtgcagcg ttggcaaccg caggggtaat tatgcaaggg
     9301 ctttttcgca acccaatggc tgaccctgga ttacttggcg tcagtagcgg ttctgcatta
     9361 atggttggcg ttgctattgt gctgcccttc tccttcccag tggtgctggt gctctatgag
     9421 caaatggtgt ttgccattgc cggaagttta gtggtctgca ccatcatttt tctcatcacg
     9481 cagcgccatc gcgatggcag catgatgcaa ttattactcg ccggtatcgc catcaatgcc
     9541 ctgtgcggcg cagcgatcgg catcctgagc tatatcggcg atgagcagca gctacgacaa
     9601 ctcacattgt ggatgatggg caatcttgga caggcgcaat ggccgacgtt attggttgcc
     9661 agttcattca tcctaccggc cattatcgca acaacttgtc tcgccggaac gctgaattta
     9721 ctgcagctcg gtgatgaaga agcccactac ctcggcgtga acgttaagcg taaacgccag
     9781 caattactgt tagtgagctc actgctcgtt ggtgctgccg tatcggtaag cggtattatc
     9841 ggctttattg gcctggtgat cccgcatctg attcgcatga ctaccggggc aaatcaccgc
     9901 tggctaatcc cttgttccgc cctcgccgga gcctgtttat tgctgatggc agacacgctt
     9961 gcccgcacgc tggtacagcc agcagaaatg cccgtgggat tattaaccag cctgcttggt
    10021 ggcccttatt ttatgtggtt gattctgcgc aaccggagga tcacatgatc agcgcccaga
    10081 atctggttta tagccttcag gggcggcgtc tgactgataa tgtctcgctg acttttcccg
    10141 gtggggaaat tgttgccatc ctcggcccta atggtgcggg aaaatctacg ctgcttcgcc
    10201 agctaaccgg ctatcttcaa cctgatagcg gcgaatgtcg gttgtttaat aagccattaa
    10261 atgaatggtc gattactgaa ctggcgaaac accgggccgt gatgcgtcaa aatagccaca
    10321 tggcgtttcc attcagcgta caagaagtta tccagatggg gcggcatccg catcgtactg
    10381 gtaatcagga taatgaaaca gcacaaataa tggcgttatg tgactgtcag gcgttagcaa
    10441 accgcgacta tcggcaatta tccggtggtg aacaacagcg cgtgcaatta gcgcgcctgc
    10501 tggtgcaact ctgggaacct actccctcac cgaaatggtt atttcttgat gaaccaactt
    10561 cggcgctgga tattcaccat cagcagcact tgtttcgttt attacgccag ttagttcacg
    10621 aacggcagtt caatgtttgc tgcgtattgc acgatctaaa tcttgccgcg cgttatgcag
    10681 accgtgttgt attaatgcaa aaaggaaagg ttatcgcaaa tggtaagccg caggacgtat
    10741 tgacacagca ggcgctgaca atgctctacg gcgcggatat taccgtactt aaagatcctg
    10801 ccaaccattc accactgatc gtcctcgatc attaaaacca acaggagtac cgcactggta
    10861 ctcctgcgaa taaataacta tacagattag ctaattgaaa cgcctttcac ccctgccata
    10921 ccttttaata atcgcaacag atcttctatc gacgtcgtgg catgaagctg gatatcaatg
    10981 gcgaccactt catgatcttc attttcctgc aacgaaacta aatccgtttt tattttttgc
    11041 tgcttaaacc agtcgagcat tgataccgta ttaccgttca ctaatgtcag ctgtaaatgg
    11101 taatttttgt tcatcaggcg gaaggttaat tgatggaaga cttccagcac cagcaaggtc
    11161 atcactgaac cataaatacc cagttcgtac ataccgctgc caataaccat accaatggcg
    11221 gcggtcaccc agatatccgc tgccgtcgtc agacctacaa tattttggtt acgaacgaga
    11281 atgttacctg caccgataaa cccgacgccc gtcacaacct gagcagcaat acggctgggg
    11341 tctagtccga cgtgacctaa agacagcacg tcagcaaaac catatttcga aacaatcata
    11401 aacagggcgc ttcccatgcc aattaataca tgtgtgcgta accctgctcc tttgccgcgc
    11461 atttgtcttt ccatgccaat agcgccacag gcaattaccg caagtataag tcgtataata
    11521 aattctgctg tcatataaaa atgcaaaaag gagcagcaag atggctcaac ttgctactcc
    11581 tttttacttg tacctcatta attcggcaag tcattagatg cttgattttt gaaggtatac
    11641 aaatttttct gcgggttttt cttgcagtat tcgatcactt taggaatttg agtgagatcg
    11701 gtttcattta aagtaacggt atcgccacct ttatatactg tttcttcatg cagcatccac
    11761 cacgcaactg gtgtcattgc ttttggattc agatcaataa attcctggca ggtcatatct
    11821 ttagcggatt cattggctgc cagcgcagat tgtgcgttca ccagtgacaa agccgctaca
    11881 gcgcccataa aaataaacgc tttacggaga gatgaaatgt tcattttgta acccattcaa
    11941 tatagagatt atatacaaac aggaggaata gctgctttta aagaagatat tccggcctgt
    12001 tcaatatgca aggaaatacg atgtaacgca ttcccggaat tacatatctt tcttaatttt
    12061 gtcccattcg cctttaactt tatctttaaa gctggctttt ttatcctgag tacaagcctg
    12121 aacgatagct ggggttacgg ttgcaatacc ctgaacatct aaaaccgcat cttctggttt
    12181 atctttgttg ttcagtgctt cagcaaaacc aactgcagtt ggctggaagg attcgtccac
    12241 agccaggaaa tcttcacagg tccaggagtt gaccggtttt ttgttatcag ctgctttttg
    12301 tgcatccgct gcattgctca ctactggcag aagaagcaga ccaccaagaa taacgcctaa
    12361 tacttttttc atcgtaatat cctcaactat aaagtgaaag agccgtcacg aatcaatttc
    12421 gacactgagg ttataacctg gttttctgta tatgtcatgt tgatggaaaa tatcaaaatc
    12481 agatattttt atttcaatac a
//
LOCUS       DAPQZD010000144        12460 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPQZD010000144 DAPQZD010000000
VERSION     DAPQZD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.146"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..138)
                     /locus_tag="RO454_003836"
     CDS             complement(<1..138)
                     /locus_tag="RO454_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDY0935598.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ET"
     gene            complement(148..699)
                     /gene="ybcL"
                     /locus_tag="RO454_003837"
     CDS             complement(148..699)
                     /gene="ybcL"
                     /locus_tag="RO454_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDY0935599.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(868..1200)
                     /gene="emrE"
                     /locus_tag="RO454_003838"
     CDS             complement(868..1200)
                     /gene="emrE"
                     /locus_tag="RO454_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDY0935600.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1544..1858)
                     /gene="fliE"
                     /locus_tag="RO454_003839"
     CDS             complement(1544..1858)
                     /gene="fliE"
                     /locus_tag="RO454_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDY0935601.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2073..3731
                     /gene="fliF"
                     /locus_tag="RO454_003840"
     CDS             2073..3731
                     /gene="fliF"
                     /locus_tag="RO454_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDY0935602.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEMEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3724..4719
                     /gene="fliG"
                     /locus_tag="RO454_003841"
     CDS             3724..4719
                     /gene="fliG"
                     /locus_tag="RO454_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDY0935603.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4712..5398
                     /gene="fliH"
                     /locus_tag="RO454_003842"
     CDS             4712..5398
                     /gene="fliH"
                     /locus_tag="RO454_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDY0935604.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5398..6771
                     /gene="fliI"
                     /locus_tag="RO454_003843"
     CDS             5398..6771
                     /gene="fliI"
                     /locus_tag="RO454_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDY0935605.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSKTHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6790..7233
                     /gene="fliJ"
                     /locus_tag="RO454_003844"
     CDS             6790..7233
                     /gene="fliJ"
                     /locus_tag="RO454_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDY0935606.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7230..8357
                     /gene="fliK"
                     /locus_tag="RO454_003845"
     CDS             7230..8357
                     /gene="fliK"
                     /locus_tag="RO454_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDY0935607.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8462..8926
                     /gene="fliL"
                     /locus_tag="RO454_003846"
     CDS             8462..8926
                     /gene="fliL"
                     /locus_tag="RO454_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDY0935608.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8931..9935
                     /gene="fliM"
                     /locus_tag="RO454_003847"
     CDS             8931..9935
                     /gene="fliM"
                     /locus_tag="RO454_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDY0935609.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9932..10345
                     /gene="fliN"
                     /locus_tag="RO454_003848"
     CDS             9932..10345
                     /gene="fliN"
                     /locus_tag="RO454_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDY0935610.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10348..10713
                     /gene="fliO"
                     /locus_tag="RO454_003849"
     CDS             10348..10713
                     /gene="fliO"
                     /locus_tag="RO454_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDY0935611.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10713..11450
                     /gene="fliP"
                     /locus_tag="RO454_003850"
     CDS             10713..11450
                     /gene="fliP"
                     /locus_tag="RO454_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDY0935612.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11460..11729
                     /gene="fliQ"
                     /locus_tag="RO454_003851"
     CDS             11460..11729
                     /gene="fliQ"
                     /locus_tag="RO454_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDY0935613.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11738..>12460
                     /gene="fliR"
                     /locus_tag="RO454_003852"
     CDS             11738..>12460
                     /gene="fliR"
                     /locus_tag="RO454_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDY0935614.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCE"
BASE COUNT         3033 a         3283 c         3311 g         2833 t
ORIGIN      
        1 cgtctcccaa ttgataactt tttttccctg acggatacga cataaagctg gcataagagg
       61 cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct ttaggttagt
      121 cttatctttt gccagcatac tcttacccta ctttatctca taaactggtg ttatctcagc
      181 ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg aattagaatc
      241 cacaggaatt ttatcagttt ttagagccca tactttaaac tggtaatgat ggggtttatc
      301 tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc ggccttgaac
      361 agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagcgggca aatatgttac
      421 agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag gagcatctgg
      481 atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc aggttaatga
      541 gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg ttgtaagttg
      601 ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcctgcg cagaaaatgt
      661 tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat tgttgtgacc
      721 tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca atgatcgcgc
      781 aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca caaatcagat
      841 tcctgttatc tcaaggataa ttatttttta atgcggtgca cttcgtgaca acaagttaat
      901 gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt ccagtcgttg
      961 accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgactcctg accagatagc
     1021 ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga atgatgcaca
     1081 atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg aaaacttcat
     1141 taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat aagggttcat
     1201 agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac acaactcact
     1261 gttttcatcc tggtgtatgt gtatccaggg ggctctggcg tggcaagaga taaaggcagg
     1321 aacaataatg atcagaagga accccaggct aacgaaagag ttcactcttc ctgggagcaa
     1381 agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat
     1441 caggtatcca ttttaaacca gaaattgagt gagtttgccc atccatgccc cacgctgtgg
     1501 gatcggatat aattaaacaa gcatagtcaa caggttacac aacctacacc tgcatggaca
     1561 tcacttcctg atacgccgcc accagcttat tacgcacctg aatccccatt tgcatagaaa
     1621 ctgaggcttt ttgcatatcg gtcatcacat cgtttagcgc cacgccgggt tcaccgaggg
     1681 tgaatttttc tgcctgcgtg cgggcggctg tttgtgtatc gcttatgcga tcgagcgcgg
     1741 cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag tgattcctgc gcacgcgcac
     1801 tcatcgccgt agcctgtaac tggctgataa ccccttcaat cccctgtatc gctgacattt
     1861 tcgtctcccg gataatttct gatagcaaag cctaccagta agtcaataag acaaaggcgc
     1921 taaatagcaa caaaaaaacg ggtttattgg cggatagaaa aaaacgaaag cacaaataat
     1981 gggagcgtca atttttcgag tttgctgacc cgggagtgag tcttgttcca ctttgccaat
     2041 aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat
     2101 ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt gttgccggtt
     2161 ccgcagcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc gactaccgca
     2221 cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa
     2281 tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaggttccg gcagataaag
     2341 ttcacgaact gcgcctgcgc ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt
     2401 tcgaactgct cgatcaggaa aagtttggta tcagccagtt cagcgaacag gtgaattatc
     2461 agcgggcgct ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg
     2521 cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt
     2581 ctgcatcggt gacggtaaat ctgttacccg gccgcgcact cgatgaaggg caaattagcg
     2641 ctattgtaca tctggtttcc agcgcggttg ctggtctgcc gccaggaaac gtcacgctgg
     2701 tggatcaggg cgggcatctg ttaacccagt ccaataccag cgggcgcgat cttaatgacg
     2761 ctcagttgaa atatgccagc gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc
     2821 tgtcgcctat tgttggtaac ggtaatattc acgcccaggt cacggcgcaa ctggacttcg
     2881 ccagtaaaga acaaacggaa gaacagtatc gccctaacgg tgatgaatct catgcggcgc
     2941 ttcgttcacg ccagcttaat gagagcgagc aaagcggttc cggttatccg ggcggcgtac
     3001 cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc accaatcagc acgcctccgg
     3061 caaatcaaaa taaccgccag cagcaggcga gcaccaccag caatagtggg ccgcgtagca
     3121 cacagcggaa tgaaaccagt aactacgaag tcgatcgcac cattcgtcat accaaaatga
     3181 acgtgggcga tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa accttgccag
     3241 acggtaaacc attgcctctc agcaacgaac agatgaagca aattgaagct ctgacccgcg
     3301 aggcgatggg cttttctgaa aaacgcggtg attcgctcaa tgttgttaac tcgccgttca
     3361 atagcagtga cgaaagcggc ggagcactgc cgttctggca acagcaagtg tttatcgatc
     3421 agttgctggc tgccggtcgc tggttgctgg tactgctggt ggcgtggttg ctgtggcgga
     3481 aagcggtacg cccgcagcta acacgtcgcg ccgaggcggt gaagactgtg cagcaacaag
     3541 cgctggcccg cgaggaaatg gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac
     3601 tccaacaacg gcgcgctaac caacgactgg gggcagaagt catgagccag cgtatccgtg
     3661 aaatgtctga taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg
     3721 atcatgagta acctgacagg caccgataaa agcgtcatcc tgctgatgac cattggcgaa
     3781 gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac ccttagcgct
     3841 gcaatggcga acgtcacgca gatctccaac aagcagctaa ccgatgtgct ggcggagttt
     3901 gagcaagaag ctgaacagtt tgccgcactg aatatcaacg ccaacgatta tctgcgttcg
     3961 gtattggtca aagctctggg tgaagaacgt gccgccagcc tgctggaaga tattctcgaa
     4021 acccgcgata ccgccagcgg tattgaaacg ctcaacttta tggagccgca gagcgccgcc
     4081 gatctgattc gcgatgagca tccgcaaatt atcgccacca ttctggtgca tctgaagcgc
     4141 gcccaggccg ccgatattct ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg
     4201 cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg cggaactgac cgaagtactg
     4261 aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca
     4321 gccgaaatta tcaacctgat gaaaacgcag caggaagaag ccgttattac cgccgtgcgt
     4381 gaattcgacg gcgagctggc gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg
     4441 gtggatgtcg atgatcgcag cattcagcgt ctgttgcagg aagtggattc cgaatcgctg
     4501 ctgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga aattcttgcg caatatgtcg
     4561 cagcgtgccg ccgatattct gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg
     4621 caggtggaaa acgaacagaa agcgattctg ctgattgtgc gccgtcttgc cgaaactggc
     4681 gagatggtga ttggcagcgg cgaggatacc tatgtctgat aatctgccgt ggaaaacctg
     4741 gacgccggac gatctcgcgc caccaccggc agagtttgtg ccaatggtcg agtcggaaga
     4801 aaccattatt gaagaggccg aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca
     4861 ggcccacgag cagggttatc aggcagggat tgccgaaggt cgccagcaag gtcatgaaca
     4921 gggctatcag gaaggactgg cccaggggct ggaacaaggt ctggcagagg cgaaggctca
     4981 acaagcgcca attcatgccc ggatgcagca actggtcagc gaatttcaaa ctacccttga
     5041 tgcgcttgat agtgtgatag cttcgcgcct gatgcagatg gcgctggagg cagcacgtca
     5101 ggtcataggt cagacgccaa cggtggataa ctcggcactg atcaaacaga tccaacagtt
     5161 gttgcagcaa gaaccgttat tcagcggtaa accacagctg cgcgtacacc cggatgatct
     5221 gcaacgtgtt gatgatatgc tcggcgcaac tttaagtttg catggctggc gcttgcgggg
     5281 cgatcccacc ctccatcctg gcggttgcaa agtctccgcc gatgaaggcg atctcgacgc
     5341 cagtgtcgcc actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg
     5401 accacgcgcc tgactcgctg gctaaccacg ctagataact ttgaagccaa aatggcgcag
     5461 ttgcctgcgg tacgtcgcta cgggcgatta acccgcgcta ccgggctggt gctggaagcc
     5521 accggattac aattgccgct cggcgcaacc tgtgtcattg agcgccagaa cggcagcaaa
     5581 acgcacgaag tagaaagcga agtcgtcggc tttaacggtc aacggctgtt tttaatgccg
     5641 ctggaggaag tggaaggtgt cctgcccggc gcgcgtgtat atgccaaaaa catttcggca
     5701 gaagggctgc aaagcggcaa gcagttgccg ctcggtccgg cgttattagg tcgtgttctg
     5761 gacggcagcg gtaaaccgct cgatggcctc ccctcccccg atacgacgga aaccggtgcg
     5821 ctgattaccc cgccatttaa cccattgcaa cgtacaccga ttgaacatgt gctggacacc
     5881 ggcgtgcgcc caatcaatgc cctgcttacc gtcgggcgcg ggcagcgtat ggggctgttt
     5941 gccgggtccg gcgttggtaa aagtgtgctg ttggggatga tggcacgtta cacccgcgcc
     6001 gatgtcattg tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag
     6061 aatatcctcg gtgccgaagg acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt
     6121 tctccgctcc tgcgaatgca aggtgccgcc tatgccacgc gcattgccga agattttcgc
     6181 gatcgtggtc agcatgtgtt gctgattatg gactccctca cccgctacgc gatggcccag
     6241 cgtgaaattg cgctggcgat tggcgaacca cctgctacta aaggttatcc accgtcggtg
     6301 tttgccaaat taccggcact ggtcgagcgt gccggaaatg gcattagcgg cggcggctcg
     6361 attaccgcgt tttataccgt gctcaccgag ggcgatgacc agcaggaccc cattgccgac
     6421 tccgcgcggg ccatcctcga cggtcacatt gtgctgtctc gccgactggc ggaagccggg
     6481 cactatccgg ctatcgatat tgaagcgtcg atcagccgcg caatgacggc gttgatcagt
     6541 gagcaacatt acgcacgagt gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac
     6601 cgagatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct cgacaaagcc
     6661 atcgccctgt ggccgcagct ggagggctat ttgcaacaag gcatttttga acgcgcggac
     6721 tgggaagcgt ctctccaggg gctggagcgt attttcccga cagtgtcata acccaggaga
     6781 taacggcaga tggcagaaca tggcgcgctg gcgaccctga aagatctggc agaaaaagag
     6841 gtagaggatg ccgcgcgcct gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa
     6901 cagctcaaaa tgctgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg
     6961 agtgccggga tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa
     7021 aaagccatta ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc
     7081 ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg
     7141 caatccacgg cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc
     7201 gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt agcgccctta attaccgccg
     7261 acgttgacac caccacattg cctggcggca aagccagcga tgctgcacaa gattttctcg
     7321 cgttgttgag cgaagcatta gcaggcgaga caactaccga caaagcggcc ccccagttgc
     7381 tggtggcaac agataagccc acgacaaaag gcgagccgct gatcagcgat attgtttccg
     7441 acgcgcaaca agcggattta ctgatccccg tggatgaaac actgcctgtc atcaacgacg
     7501 aacaatccac atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg
     7561 acaaaaatac gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagcc
     7621 tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac
     7681 cgtcaaccgt gttaccggca gagaaaccaa cgcttttcac aaaactgact tctgcgcaac
     7741 tcacaacagc acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg
     7801 tagcagaagc ccagagtaaa gcggaagtca tcagcacacc ttcgccggtg actgctgccg
     7861 ccagcccgct aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt
     7921 tgagtgcacc gctgggttct cacgaatggc aacaatcatt aagccagcat atttcgctgt
     7981 tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag
     8041 tgcaaatttc cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc
     8101 agcacgtacg cgcagccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa
     8161 gtggcattca gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg
     8221 ccgcttccca gcaacagcaa agccaacgca cagcaaacca tgaacctctg acgggggaag
     8281 acgacgatac gcttccggtt cccgtctctt tacaagggcg tgtaacaggc aacagcggcg
     8341 ttgatatttt cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt
     8401 tgcgctcaag acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca
     8461 catgactgat tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt
     8521 attcattacc ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt
     8581 tgccgctgac gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga
     8641 tacctttacg gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg
     8701 actgaaagat gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg
     8761 cttgctgtta ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa
     8821 aaacctgatt gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa
     8881 acaggatgtc accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata
     8941 gtattctttc tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg
     9001 aaccgacagc cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac
     9061 gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc
     9121 attttcgtat ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca
     9181 tccgcattca gccgtaccat gaatttgccc gcaacttgcc ggtgccgacc aacctgaacc
     9241 ttatccacct taaaccgctg cgcggcacag ggctggtggt gttctcgccg agcctggtgt
     9301 ttatcgccgt ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc
     9361 gcgagtttac tcataccgaa cagcgcgtca tcaaccgcat gttaaagctg gcgcttgaag
     9421 gctatagcga cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa
     9481 tgcaggtgaa atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt
     9541 tccatgtgga gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga
     9601 tcgagccgct acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc
     9661 agaactggcg cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca
     9721 actttgccga tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc
     9781 tgccgataga aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca
     9841 gtcagtacgg cactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga
     9901 ttttaaattc tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga
     9961 caacaacggc gcaatggacg atatgtgggc tgaagcgttg agcgagcaaa aatcaaccag
    10021 cggaaaaagc gctgccgacg caatgttcca gcaatttggc ggcggagatg tcagcggaac
    10081 gttgcaggat atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg
    10141 tacgcggatg accatcaaag agctgttgcg tctgacgcaa ggatcagtcg tggcgctgga
    10201 cggtctggcg ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga
    10261 agtggtggtt gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga
    10321 gcggatgcgc cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg
    10381 ccggtttctg ctgcgccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg
    10441 atcctcgctg ccgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt
    10501 aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc
    10561 gatgtggaag atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctacat
    10621 aaacttcccc cttcagcacc aacggaggag ataccgcaga ccgattttca gtcggtcatg
    10681 aaaaatttgc ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg
    10741 tccttctctg gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc
    10801 cgctacctgg cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct
    10861 cgttgacgtt cattccagcg attttactga tgatgaccag tttcacccgc atcattattg
    10921 tttttggttt attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc
    10981 tgggactggc actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg
    11041 tcgatgcgta ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag
    11101 gggcgcagcc gctgcgtgag tttatgctgc gtcagacccg cgaggcggat ttagggttgt
    11161 ttgccagact ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt
    11221 tgctcccggc ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt
    11281 tcatcccttt tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga
    11341 tgatggttcc cccagccacc attgctctgc cctttaaact gatgctgttt gtactggtgg
    11401 atggctggca attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa
    11461 tgacacctga atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg ctggcactgg
    11521 ctgccccact gttgctggtg gcgctggtca cgggtcttat catcagtatt ttgcaggccg
    11581 ccacgcagat caacgaaatg acgctgtcgt ttattccaaa aatcatcgcc gtatttatcg
    11641 ccattattat tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt
    11701 tcactaacct gccgtatatc atcgggtagg ccgtacaatg atgcaggtaa caagcgatca
    11761 atggctttcc tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc
    11821 taccgcgcca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat
    11881 gatgatcacg ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    11941 ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg gttttaccat
    12001 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    12061 gtcatttgcg acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat
    12121 catggatatg ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    12181 actgctggtc gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc
    12241 gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt
    12301 accgctcatt actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc
    12361 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt
    12421 aatggcggca ttaatgccgt taattgcgcc tttttgcgaa
//
LOCUS       DAPQZD010000145        12382 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPQZD010000145 DAPQZD010000000
VERSION     DAPQZD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.147"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            29..1543
                     /gene="caiT"
                     /locus_tag="RO454_003853"
     CDS             29..1543
                     /gene="caiT"
                     /locus_tag="RO454_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDY0935615.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKY"
     gene            1574..2716
                     /gene="caiA"
                     /locus_tag="RO454_003854"
     CDS             1574..2716
                     /gene="caiA"
                     /locus_tag="RO454_003854"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDY0935616.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2845..4062
                     /gene="caiB"
                     /locus_tag="RO454_003855"
     CDS             2845..4062
                     /gene="caiB"
                     /locus_tag="RO454_003855"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDY0935617.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4135..5688
                     /gene="caiC"
                     /locus_tag="RO454_003856"
     CDS             4135..5688
                     /gene="caiC"
                     /locus_tag="RO454_003856"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDY0935618.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDIYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSVRAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSVNDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5797..6582
                     /gene="caiD"
                     /locus_tag="RO454_003857"
     CDS             5797..6582
                     /gene="caiD"
                     /locus_tag="RO454_003857"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDY0935619.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6588..7178
                     /gene="caiE"
                     /locus_tag="RO454_003858"
     CDS             6588..7178
                     /gene="caiE"
                     /locus_tag="RO454_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDY0935620.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVLAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7297..7692)
                     /gene="caiF"
                     /locus_tag="RO454_003859"
     CDS             complement(7297..7692)
                     /gene="caiF"
                     /locus_tag="RO454_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDY0935621.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7953..11174)
                     /gene="carB"
                     /locus_tag="RO454_003860"
     CDS             complement(7953..11174)
                     /gene="carB"
                     /locus_tag="RO454_003860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDY0935622.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11192..12340)
                     /gene="carA"
                     /locus_tag="RO454_003861"
     CDS             complement(11192..12340)
                     /gene="carA"
                     /locus_tag="RO454_003861"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDY0935623.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         2872 a         3252 c         3289 g         2969 t
ORIGIN      
        1 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
       61 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
      121 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
      181 gggatgggca tttgaatggt atatggtggt gatgcttttc ggctggttct ggctggtgtt
      241 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacca gaatttagta ccgccagttg
      301 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
      361 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcaa caggcgcgaa
      421 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
      481 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
      541 cagctcgacc ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
      601 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
      661 cacgccgctg gtaaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
      721 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
      781 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
      841 ttgggtgttc atcgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
      901 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
      961 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttacg ctatccagat
     1021 gagtatcttc ctcgcccgca tctcccgtgg tcgtaccgtg cgtgaactgt gcttcggcat
     1081 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacaccct
     1141 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
     1201 gcgcgccatc atcgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
     1261 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
     1321 gatgtccact tgccgcgaag tacgtgacgg cgaagaaccc ccgctgctgg tgcgcattgg
     1381 ttggtcagtt ctggttggca ttatcggcat tgttctgctg gcgctcggcg gcctgaaacc
     1441 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca tcatggtgac
     1501 gctctccttt attaaagacg cgaaacagaa ctggaaatat taattaaccc caaaaatatc
     1561 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
     1621 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
     1681 cgtctacccg gaacgttttg tcaaagccct ggcggatatg ggtatcgaca gtctgctgat
     1741 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
     1801 actgggtcgt ctgggagcgc caacttacgt gctgtatcag ttaccgggcg ggtttaacac
     1861 cttcctgcgt gaaggtacgc aagagcagat cgacaagatc atggctttcc gcggcaccgg
     1921 taagcagatg tggaactcag cgattaccga accgggcgca ggctccgacg tgggtagcct
     1981 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
     2041 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
     2101 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
     2161 acttgagaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgatgtgga
     2221 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
     2281 gttcgaccat gaacgtttcc tggtagccct gaccaactac ggtacggcga tgtgcgcctt
     2341 tgaagatgcg gcgcgctacg ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt
     2401 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
     2461 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
     2521 gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct
     2581 gggcggtgtc gggattgcgg gtaatcaccg catcagccgc ttctggcgtg acctgcgtgt
     2641 agaccgcgtt tccggaggat ctgacgaaat gcagatcctg acgctgggcc gcgcggtgct
     2701 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc
     2761 tacaggagca cgaatgtcgg ccggataagg cgtttacgcc gcatccggca gtcatgcgct
     2821 acataaattt ttcaggagct aataatggat catctaacca tgccgaaatt cgggccgctg
     2881 tccggattgc gcgttgtctt ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg
     2941 ttcgcagaat ggggcgcgga agttatctgg atcgaaaacg tcgcctgggc cgacaccatt
     3001 cgcgttcaac cgaactaccc gcaactttcc cgccgcaacc tgcacgcgct gtcgttaaat
     3061 atcttcaaag atgaaggtcg tgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
     3121 atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtcctgtgg
     3181 cagcacaacc caaaactggt tatcgctcac ctgtccggtt ttggtcagta tggcaccgag
     3241 gagtacacca atcttccggc ctataacact atcgcccagg ccttcagtgg ttacctgatt
     3301 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
     3361 ggcctgactg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggcaaa
     3421 ggcgaaagca tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
     3481 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga ccaaagggaa agatccctac
     3541 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
     3601 attactcaaa ttgcagagtg ctttaaagat attggcctcg cacatctgct cggcacgcca
     3661 gaaattccgg aaggcaccca gcttatccac cgtatcgaat gcccttacgg cccactggta
     3721 gaagagaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
     3781 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
     3841 cagtatgtgg ctcgcgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
     3901 gggccgaata tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
     3961 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
     4021 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcag
     4081 cttgcctgac tagtgcccgc tcaataacaa acgaacaaaa tggatagagg tgcaatggat
     4141 atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt ttacggtcat
     4201 aaaacggcgc tgatttgtga atccagcagc ggagtcgtta accggtatag ttatcttgag
     4261 ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat tcgcaaaggc
     4321 gacaaggttg ctctacatct cgacaactgc ccggaattta tcttttgctg gttcgggttg
     4381 gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttgcgcga agaaagcacg
     4441 tggatcctgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt ttatcctatg
     4501 tatcaacaga ttcagcagga agatgcctct cagttgcggc acatttgcct gacagatatg
     4561 gcacttcccg ctgatgatgg cgtgagttcg tttactcaac tgaaaaatca acaacctgcc
     4621 accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct cttcacctcc
     4681 ggcaccacct cccgaccgaa aggggtggtg ataactcatt acaacctgcg cttcgctgga
     4741 tattactccg cctggcagtg cgcactgcgt gatgatgaca tctacctgac ggtaatgcct
     4801 gcatttcata tcgattgcca gtgtactgcg gcaatggcgg cattttctgc cggggccacc
     4861 tttgtgctgg tcgagaaata cagcgtccgc gccttctggg ggcaggtaca gaagtaccgt
     4921 gccaccgtta ccgaatgtat tccgatgatg atccgtaccc tgatggtaca accaccttct
     4981 gtgaatgaca ggcaacactg cctgcgggaa gtgatgtttt atctcaacct gtcagagcag
     5041 gaaaaagacg ctttttgtga acgcttcggc gtccgcttgc tgacgtctta cgggatgacg
     5101 gaaaccattg ttggcattat cggcgatcgc cctggcgata aacgacgctg gccgtcaatt
     5161 ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg cccgctcccg
     5221 gcaggtgaaa tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat cttcaaagag
     5281 tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgatggctg gctacatacc
     5341 ggcgataccg gataccgcga cgaagagggc tttttttatt tcgtcgatcg ccgctgcaat
     5401 atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tcgaaaatat catcgccacc
     5461 catcccaaaa ttcaggatat cgtggttgtg ggtattaaag attcgattcg cgatgaagcc
     5521 atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga atttttccgc
     5581 ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat cagaaaagat
     5641 ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat gaaacggcag
     5701 ggaactaccc tgtcagctaa taatcacgct ctaaaacaat atgcattttt tgcagggatg
     5761 ttgtcgtccc tgaaaaagca aaaatggaga aaaggaatga gtgaatcatt acatctgacc
     5821 cgcaatggat caattctgga aattaccctt gatcgtccaa aagcgaatgc tattgatgca
     5881 aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc gcaattacgt
     5941 gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga tttaaaagcg
     6001 gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gaggttttgc gggattaacc
     6061 gaaattttca atctcgacaa accggtcatc gccgctgtga acggctatgc ctttggcggc
     6121 ggctttgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc cagcttcgcc
     6181 ctaccggaag ccaaactggg catcgttcct gacagcggcg gcgtgctgcg tctgccgaag
     6241 atcctgccgc ctgccatcgt caatgaaatg gtgatgaccg gcagacgaat gggcgcagaa
     6301 gaggcgctgc gttggggggt agtcaaccgc gtggttagcc aggcggaact gatggataac
     6361 gcccgcgaac tggcgcagca gctggttaac agcgctccgc tggcgattgc ggcgctgaaa
     6421 gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta tattcgcagc
     6481 ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga agggccgctg
     6541 gcgtttgccg agaagcgcga tccagtgtgg aaaggacgtt aacaaccgtg agctattacg
     6601 cctttgaggg gttaattccg gttgttcacc cgacggcgtt tgtccatccc agtgcagtat
     6661 tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc tcactgcgtg
     6721 gtgactacgg gcggttgatc gtgctagcgg gagccaatat tcaggatggc tgcattatgc
     6781 atggctactg cgacactgac accatcgttg gggaaaacgg tcatatcggg cacggagcga
     6841 tcctgcatgg ttgtgtgatt ggtcgcgatg cactggtcgg gatgaacagc gtgattatgg
     6901 atggcgcggt cattggcgaa gagagcattg ttgccgctat gagctttgtc aaagcgggct
     6961 ttcgcggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta cgcagcgtga
     7021 gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat cttgtcgggc
     7081 gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa aatcgccccc
     7141 gtttgcaggg gacgacggat gtgacgccga aacggtgatc ttgccggatg cggcgtaaac
     7201 gccttatccg acctggctgg aaagcaaccg taggccggat aagatgcgcc agcatcgcat
     7261 ccggcaacca ggctgcgtgg tatcaacgaa atgacattaa cgacgcatac tctttgacaa
     7321 catcatctgc cacttctgct cgcggttaag ttccgtgagc ggaacggcag gaatacgcgg
     7381 cgtctttctt acgccgacta atttatcccg gctgttattg cgcagacgcg cgtaaatttg
     7441 ctcatcgata tcaaccactt taaccagtcg ctgacactgg catccccgcc cttccagctt
     7501 attagggatc atcttaactt cgcagcttat ttctgtgact tccgacagaa tataagtcag
     7561 ggtattaacc gccttgctgt gttcaatatc gaaatgaata gagatctctc ttgctatcac
     7621 ccaccgattt tcagccatca tccattcggc gattaacaag tagagtggtt tttcaacata
     7681 tccttcacac atacgaactc cagtttcaca aattaaaaga ctggctatga ctcgttttgc
     7741 agcaattaaa caaggccata ttaaaattgt ggatatatcg atagtatgca acttcaccgt
     7801 cgataagtgt gagctaaatc ctgcttatgt catcaaaaat atcgattccc caaaagattt
     7861 acttaatggg caaaaaaaac ggtcctcatc agagaaccgt attagttatt tgatcaaatt
     7921 agcggatgaa aaatatccgc catgacacgc tattatttga tctgcgcgtg catttcctgc
     7981 accgaaatta ctttttcggt cgcatcggca ttcagcgcca tcgcggtggc aaagccgccg
     8041 ttcagggtgg tgtcgtaatg cactttatat tgcagcgcac tgcgacggat cacgcgggag
     8101 tcttcaatcg cacgacgacc tgaggtggtg ttgatgatgt aggtatattc gccattctta
     8161 atacggtcct gaatgtgcgg acggccttca tgcaccttgt tcaccagacg cggattgata
     8221 cccgcttcgc ccagcacaat cgccgtgccg tgagtcgcat ccagctcgaa gccctgtttc
     8281 agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac ggaaagcagc
     8341 gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc aaacgcttca
     8401 gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc gcatttctgg ccctaacagc
     8461 gggtcaacgc ccgggaattt attgaacggc agcaccactt ctttcaccga gtagtacggc
     8521 gggataactt ctttggttac gccctgctca gccagcgatt tgccagccat cacacgcgcc
     8581 gccacttttg ccagcggtac gccggtggct ttggagacga acggaacggt acgcgccgca
     8641 cgcgggttaa cttcaatcag gtagacttcg ttgtttttca ccgcaaactg cacgttcatc
     8701 aggccgcgca cctgcagttc gaaggccagt ttctgcacct gctggcgcat cacatcctga
     8761 atttcctgac ttaaggtgta ggctggcagt gaacatgcgg agtcaccgga gtgcacaccc
     8821 gcctgctcaa tatgctccat gatgccgcca atcagcacca tttcgccgtc gcagatggca
     8881 tccacgtcaa cttctactgc gtcatcaagg aagtggtcca gcaacactgg cgcatcgtta
     8941 gatacgctga ccgctgtctg gaagtagcga cgcaggtcag cttcgtcata gacgatttcc
     9001 atcgcccgac cgccgagaac gtaagacgga cgcaccacca gcgggtagcc aatctctttc
     9061 gctttctcaa ccgccatttc gatggtggta acggtggcgt tcgcaggttg tttcagtttc
     9121 agacgctcaa ccgcatgctg gaagcgttca cggtcttccg cacggtcgat ggcatccggg
     9181 ctggtaccga taaccggtac gccagcagct tccagcgcgc gcgccagttt cagcggggtc
     9241 tgaccgccgt actggacgat aacgcctttc ggcttctcaa tacgcacaat ttccagcacg
     9301 tcttccagcg ttaccggctc gaagtagaga cggtcagagg tgtcgtagtc ggtggagacg
     9361 gtttccgggt tacagttaac cataatggtt tcataaccat cttcgcgcag cgccagcgag
     9421 gcgtgtacac agcagtagtc gaattcgata ccctgaccga tacggttcgg gccgccgccg
     9481 agcaccatga ttttttcacg atcggtggac ggattcgctt cgcactcttc ttcataagtg
     9541 gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac aggtatccac gcgcttataa
     9601 accgggtgca agtcatactg gtcgcgcagc ttgcggattt ctgcttcacg cacgcccgcg
     9661 agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg
     9721 ttcaggccag tgatgcccac ttctgcgact ttctcttcca gacgcaccag ctcttcaatc
     9781 tgtaccagga accagcggtc aatgttggtc aaattaaaca cgccgtctac agacaggccc
     9841 gcgcggaagg catcagcgat ataccagata cgctctgcgc ctgcatcttt cagttcgcgg
     9901 cggattttag tcagcgcttc cgggtcgtcg agactcactt tcggatcgaa gccagttgcg
     9961 cccacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg accaatcgcc
    10021 atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc ggcgaatttc
    10081 tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc gaaggaggcc
    10141 ggagtacgcc caccggtgat gtcgttcatc agctcgtcga gggtgtaacc caccgccagt
    10201 ttcgccgcca ctttggcaat cgggaaacca gtggctttcg acgccagcgc agaggagcga
    10261 gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt caccgcaaac
    10321 tggacgttgg aaccaccggt ttcaacgccg atttcacgca gtaccgccat cgaggcgtta
    10381 cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt gatggagtca
    10441 ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat acagttgtcg
    10501 tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga ctcatcaatc
    10561 agcagctctt tagtcggaga aagatccaga ccgcgggcgc aaatttcttc aaactcttcg
    10621 cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg aataatgcac
    10681 gggaagccaa cttcagcggc aaccgccagc gcttcttcca ttgaatgagc gataccggaa
    10741 cgcgcagttt ccagaccgat tttcttcatc gctacgtcga aacggcggcg gtcttcggct
    10801 ttatcaatcg catcggcagt ggcaccaatc atggtgacgc cgaactcttc cagcacgccc
    10861 tgacgctcaa gctccagcgc acagttcagc gccgtctgcc cccccatggt tggcagcacc
    10921 gcgtccgggc gctctttttc aataatcttg cgtaccactt cccagtgaat cggctcgatg
    10981 taggttgcat cagccatttc cgggtcggtc atgatggtcg ccgggttgga gttcaccaga
    11041 atgacgcggt aaccctcttc acgcagggct ttacacgctt gcgcgccaga gtagtcaaac
    11101 tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact ttttatatct
    11161 gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg tactgctcaa
    11221 ttaacgcgat aaagtggtca aacaacggcg cggcgtcgtg tggaccaggg ctggcttccg
    11281 ggtgtccctg gaagctgaac gccggtttat cggtacgatg aatgccctgt aacgtaccgt
    11341 cgaacaggga tttatgtgtg acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa
    11401 aaccgtggtt ctgggcggtg atcatcacca catttttctc aacatcttta accggatggt
    11461 tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc agcgccagca
    11521 gctgatggcc gaggcagatg ccgaataccg gaatatcggt ttcgaggaat ttctggatgg
    11581 cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg aagatgccgt
    11641 ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg gtcagacgac
    11701 agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca taagccacga
    11761 cgtggtacgg cagctcgtct tcttttttcg cttctggcag accgccggtc agcgtccagc
    11821 tcccttgtgt ccagctatag ggttctgcgg tagtcacttc ttttgccaga tccatgccat
    11881 tcaggccagg gaacgcgcgg gctttttcta acgccagcgc cgcatccggg ttatcgcctg
    11941 cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat
    12001 cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg tcttcggtat
    12061 tacgaaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgg gcatgtacct
    12121 gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga taagtaagag
    12181 taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg gtcattgaag
    12241 tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga ccgtgaaact
    12301 gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc agagaatatt
    12361 cactcacttt atttgcatat ta
//
LOCUS       DAPQZD010000146        12256 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPQZD010000146 DAPQZD010000000
VERSION     DAPQZD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.148"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(224..1477)
                     /locus_tag="RO454_003862"
     CDS             complement(224..1477)
                     /locus_tag="RO454_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935624.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            1584..2477
                     /locus_tag="RO454_003863"
     CDS             1584..2477
                     /locus_tag="RO454_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0935625.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2612..3832
                     /gene="mlc"
                     /locus_tag="RO454_003864"
                     /gene_synonym="dgsA"
     CDS             2612..3832
                     /gene="mlc"
                     /locus_tag="RO454_003864"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDY0935626.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3957..4652
                     /gene="bioD"
                     /locus_tag="RO454_003865"
     CDS             3957..4652
                     /gene="bioD"
                     /locus_tag="RO454_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDY0935627.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4605..5897)
                     /gene="clcB"
                     /locus_tag="RO454_003866"
     CDS             complement(4605..5897)
                     /gene="clcB"
                     /locus_tag="RO454_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDY0935628.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(6058..6672)
                     /gene="dmsD"
                     /locus_tag="RO454_003867"
     CDS             complement(6058..6672)
                     /gene="dmsD"
                     /locus_tag="RO454_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDY0935629.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6715..7569)
                     /locus_tag="RO454_003868"
     CDS             complement(6715..7569)
                     /locus_tag="RO454_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDY0935630.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7571..8188)
                     /gene="dmsB"
                     /locus_tag="RO454_003869"
     CDS             complement(7571..8188)
                     /gene="dmsB"
                     /locus_tag="RO454_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDY0935631.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8199..9768)
                     /locus_tag="RO454_003870"
                     /pseudo
     CDS             complement(8199..9768)
                     /locus_tag="RO454_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(9829..12255)
                     /locus_tag="RO454_003871"
     CDS             complement(9829..12255)
                     /locus_tag="RO454_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDY0935632.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDKYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT         3094 a         3096 c         3138 g         2928 t
ORIGIN      
        1 tgttaactat tttactactt acaaagcggt agaataactg cgcatcagta ataaatgaca
       61 cacagcaaaa tgaatccgtt tatttgggta cttataatcc tgatgacgct agatgcgctg
      121 cgggaattgg ctggcgcttc gtctatttta ggatggctat taacgttggt ttgagcaggc
      181 aataagtccg gacgggtatt tacctcagtc cggacttatt ttttcaggcg tgcagacgat
      241 gatgcaaacg cgtcccgacc agcagagcaa tgaccagcat cagagcaata aatgcgccga
      301 cgccgttcca gccatagtta tgccagaaaa caccacccag cgtcccggca atactcgacc
      361 ccagatagta actgaacaga tacagcgagg aggcctggcc tttagcgcgt tttgcgcggg
      421 ggccgatcca gctgctggca acggagtggg ctgcgaagaa tccggctgag aagagtaaca
      481 ttccggcaaa gatcagccac agcgagctga ataaggtcat cagtaaacca aacagcataa
      541 cccccgtcga aaacaacatc accggaccgc gtccatagcg ggcggtcatg gttccggctt
      601 tgggtgagct ccatgtaccg gtcaaataag ccagcgataa taagccaacc acggcctgac
      661 tgacatgcca gggggaaagc atcaacctat agccgatgta attaaacagc gtgacgaacg
      721 accccatcag caaaaagcct tctgcgaaca ataacggtaa tccccggtca cgccagtgca
      781 gacgaaagtt gataaataac gtcttagggc gcagcgaagt cgggcgaaaa tggcgtgatt
      841 cagggaggat tttccagaac atcaacgccg aggccagcgc gaaacaaccg attgccgcca
      901 gagcaattcg ccagttaaaa aagtccgtga agacaccgct aattaagcgt ccgctcatgc
      961 cgccaattga gttgccgctg atatacaacc ccattgaaaa ggccacgaaa ctagggtgga
     1021 tttcctcgct aagataagtc atgccaacgg ctgccacgcc acttaacgaa agcccaatca
     1081 aggcgcgcat aatcaaaatg ccgtgccagc tggtcatcat tgtcgaaagt aacgtacaaa
     1141 tggaggccaa cagtagcgcc gtgaccatca ctggtttgcg accaatggca tcggatagcg
     1201 ggccagtaaa cagcaaaccg atagccaaca tcgccgttga aatggacagt gaaatactac
     1261 tgttcgcagg cgttaaacca aactcctgcg aaagcaccgg aaggataggt tgcacacaat
     1321 agagaagtgc gaaggttgcc agtccggcag agaaaagcgc cagggtgacg cgcataaatt
     1381 gcggcgtacc gcgtttaata aaatgatttg gctgagaaat gctttgcttg tcagtgtcgc
     1441 ttgccggagc gccatcaaca gttgtagtac ggctcacttg aaatccttgc taaatatgcc
     1501 tgtggatcag gcttatgaat agattaggaa aatcgaattg ttctgtctaa tatattaata
     1561 atctcaaata agatgtttta aatatgaata ttgaacttcg tcatctgcgt tactttgttg
     1621 ctgttgcaga agagctgcat ttcgggcgcg ccgctgcccg cctgaatatt tcgcaaccac
     1681 cgctaagtca gcagattcag gcgctggagc aacaaattgg tgcccgactg ctggcacgga
     1741 ccaatcgcag tgtattgctg acggaagcag gaaaacagtt tcttgtagat agtcggcaaa
     1801 tcctgtctat ggtggatgat gctgccgctc gcgctgaaag gctgcatcag ggcgaagcgg
     1861 gggagttgcg cattggtttt acttcgtccg ctccttttat tcgggcggtg tccgatacgt
     1921 tatcgctgtt tcgccgtgat tatcccgatg tccatttaca aacccgcgaa atgaacactc
     1981 gcgagcaaat cgctccgctc attgaaggaa cgctggatat gggattgctg cgtaacacag
     2041 cgttaccgga gacgcttgaa cacgcagtca tcgtccatga accgcttatg gcgatgatcc
     2101 cgcacgatca tcccctggca aataacccga gtgtaacgct ggctgaactg gcgaaagaac
     2161 cctttgtctt ttttgatccg cacgtcggaa cagggctgta tgacgatatt ctcgggctga
     2221 tgagacgtta caatttgacg cccgtcatca ctcaggaggt gggtgaggca atgaccatca
     2281 tcggtctggt ttccgctggt ctgggtgttt caattttgcc tgcgtcattt aaacgtgttc
     2341 agctcaacga aatgcgctgg gtgccgattg ctgaagagga tgcggtttct gaaatgtggt
     2401 tggtctggcc gaaacatcat gaacaaagtc cggctgcgcg taactttcgt attcatctgc
     2461 tgaatgctct caggtgaggg aaatttcagc gaaaaagccc gaaaaatgtg ctgttaatca
     2521 catgcctaag taaaaatttg acgacacgta ttgaagtgct tcaccatagc ctacagatta
     2581 tttcggagcg cgaaaatata gggagtatgc ggtggttgct gaaaaccagc ctgggcacat
     2641 tgatcaaata aagcaaacca acgcgggcgc agtttatcgc ctgattgatc agcttggtcc
     2701 agtctcgcgt atcgatcttt cccgtctggc gcaactggct cctgccagta tcactaaaat
     2761 tgtccgtgag atgctcgaag cacacctggt gcaagagctg gaaatcaaag aagcggggaa
     2821 ccgtggccgt ccggcagtgg ggctggtggt tgaaactgaa gcctggcact atctttctct
     2881 gcgcattagt cgcggggaga ttttccttgc tttgcgcgat ctgagcagca aactggtagt
     2941 ggaggagtcg caggaactgg cgttaaaaga tgactcgcca ttgctggatc gtatcatttc
     3001 ccatatcgat cagtttttta tccgccacca gaaaaaactt gagcgtctga cttcgattgc
     3061 cataaccttg ccgggaatta ttgatacgga aaatggtatt gtacatcgca tgccgttcta
     3121 cgaggatgta aaagagatgc cgctcggcga ggcgctggag cagcataccg gcgtaccggt
     3181 ttatattcag catgatatca gcgcatggac gatggctgag gccttgtttg gtgcctcacg
     3241 cggggcgcgc gatgtgattc aggtggttat cgatcacaac gtgggggctg gcgtcattac
     3301 cgatggtcat ctgctacacg caggcagcag tagcctcgtg gaaataggcc acacacaggt
     3361 cgacccgtat gggaaacgct gttattgcgg gaatcacggt tgcctcgaaa ccatcgccag
     3421 tgtggacagt attcttgagc tggcacagct gcgtctcaat caatccatga gctcgatgtt
     3481 acatggacaa ccgttaaccg tggactcatt gtgtcaggcg gcattgcgcg gcgatttact
     3541 ggcaaaagac atcattaccg gggtaggcgc gcatgtcggt cgcattcttg ccatcatggt
     3601 gaatttattt aacccacaaa aaatactgat tggctcaccg ttaagtaaag cggcagatat
     3661 cctcttcccg gtcatctcgg acagcatccg tcagcaggcc cttcctgcgt atagtcagca
     3721 cattagcgtt gagagtactc agttttctaa ccagggcacg atggcaggcg ctgcgctggt
     3781 aaaagacgcg atgtataacg gttctttgtt gattcgtctg ttgcagggtt aacatttttt
     3841 aactgttcta ccaaaatttg cgctatctca atttgggcca ggaaagcata acttagactt
     3901 tcaaggttaa ttattttcct ggtttatatt tgtgaagcat aacggtggag ttagtgatgc
     3961 tgaagcgttt ctttattacc ggtacagaca cttctgtagg gaaaacggtg gtttcccgcg
     4021 cattgctaca agcgttagcc tcccagggaa aaacggttgc gggatataaa cccgtagcga
     4081 aggggagcaa agagacaccc gaagggctgc gtaataaaga tgccctggtg ttgcagagtg
     4141 tttcaaccat cgaactgcct tatgaagcag ttaatcctat cgcgttaagc gaagaagaaa
     4201 gtagcgtggc gcacagttgc ccaatcaatt acaccctcat ttcaaacggc ctggcaaacc
     4261 tgaccgataa agtcgatcat gtcgtggtag aagggactgg cggctggcgc agtctgatga
     4321 atgatttgcg tccgctctct gaatgggtgg tgcaggaaca actgccggtg ttgatggttg
     4381 tcggtattca ggaaggttgc attaaccatg cactgctaac agctcaggcg attgccaacg
     4441 acgggctgcc gctcattggc tgggtggcta accgaatcaa cccaggactg gcgcattatg
     4501 cggaaatcat tgatgtgctc ggtaaaaaac ttccggcacc gctcattggt gaactgcctt
     4561 atctgccgcg cgccgaacag cgtgaactgg ggcaatacat ccgcttagct atgcttcgca
     4621 gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg aaattaccga cgcaattacg
     4681 caggcaatca ataaaccggg gagtagctga tactccccgg tcatttcaca tatcatcaac
     4741 gtcgacatag tcggcgcgtg cgtggtcgcc gccaacagtg tcgccatccc ggtcaatccg
     4801 agtaaaagcg taatttcttc gccatcaggt aaccataatc ccaggctacg accatacaac
     4861 atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga caccaccggg cgcgccagaa
     4921 ccactactcg ccagcacggc acacagttta cagaggaaga tcccggcaat gatcattaac
     4981 agtggcggag cggttaaaaa ggattgtacg gtgctatagc cgttgcccca cactgcaggt
     5041 gtgaacaggg aaagaagacc cacgatcaac ccgcccagtg ccagttgcca gggcggcgca
     5101 agcttgagac tcacaaatcc acgatgacag gcgttcatta acgttaacaa cagtggtccg
     5161 cacagacctg ccagcacacc tgtactgata atcagcgcat agtcacgagc ctgaaccgtc
     5221 actgaaagtt gtacgctgta gagtaatgcg tcgctatgat taataagatt gctaaccagc
     5281 aacgccacga cggcggaaat aatcaccggg ccgagagagg ccaacatcat agtgccaaac
     5341 agcacttcgg ctataaataa actaccagca agcggggcgc gataggccgc agccattccc
     5401 gccgcagctc cacaggcgat ccataatttc cactcctggc gtggcgtaaa acgttgggca
     5461 aaacacgagg ctgcaagggc agctaaaaga atcatcgcac cttcgcgacc aattgcactg
     5521 ccgctggtta ctaccagcag agaggcaagc gatttaacca gacttgctgc gtaatcaaac
     5581 tgaccatcgg tttgcaacgc ttccatgtaa tcggtcggcg catgagggcg ttgctgggta
     5641 aatttctgcc agcccatcag caacaaaccc gccgccagtc cgccgagcgc cggggttagc
     5701 agccgtcgcc acggggaaag atttgtcgct gcattgacca gactgccaga gtcattattg
     5761 aggaacagcc actccagtag cagcatcgca tgacgaaacc cggcaacggc aaaggccgca
     5821 agaataccga cgattgttgc gataagcaga cggcggaaca tagtgcgcag atccggatag
     5881 atgtgtagat gatgcatgag cagaaatggg caatgaaaca cgggcgacta ttttgcggca
     5941 aaagaggcca gccgcaaaag cagtgtgtgc tttaagagat aaaaaaaacg tgacactgta
     6001 acctattaat tgccgaattg cgtgtaatcg tcaccatccg gcaataatac ggtgatccta
     6061 tcgaaacagc ggtttaaccg cgacaggaat caacagttgt gattgccatt gcgccagcgt
     6121 taagcgagcg agttcaccca gtgcacggta gaaagggtgt tctgcttttt cgataaaaac
     6181 gtcaagaaaa cgtgttgacc acggaaaaag gtgccatgcc agcaattctt cgcattctgt
     6241 ctggcgacca ttctccgcca accacgcagc catcagcagc agtgaaccaa aattatcttc
     6301 cggttcgttt tgcttcattt cgaactgaat gcctttctcg cgcatccact gacgaagtgc
     6361 caatgttgaa tcgccaaaca gcacagattc gcgatccagc cagaccgatc cccacggcgg
     6421 agacggcagt gcccacgggc cgacaaacaa acgctgccag gcctgggcgt gagtctcttc
     6481 acattgtgtc tgaaaagccg tcaccagcgg cgctaatgac gcttctggta gaggccactg
     6541 agtttcccag ccgtcattgg tgagtaccgc aaccagaggt gcggcttccg cactctctgg
     6601 agcgtaataa aacagcgcgc ccaacacgcg cgccgcaaca gaaaaattat cttgctgtga
     6661 aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc cccgcaccaa tttgttaacc
     6721 tgcaatagcc attcctaccg tcatgtgcag gccataaaag agcacacgcc cgatcatctc
     6781 accgccaaga atgaggatta gccccagaac aagtccggca acgtgaggtt cacggcgacg
     6841 aatgagcgga caaagccagc atccaagacc tgcgcataac aataccacac gccagacctg
     6901 taaggaggcg taatctggta ccagtgcgct ggcttgttgt acggatgagt gaatagacgc
     6961 cagagacaaa ccttgcagca caatcaaccc ggcacaagcg atcagcgcca gaatgctaat
     7021 gatggcaaat ggtgtggtat taaatgtaac gcgggctgca cgcagaattg tggcagcaag
     7081 tattggaccg ctcaacaata ccgtcaggaa gaaagccagc gtagtgtaac cgttatgcca
     7141 ggttggcaca gtgtcgattt gatacacgca ggtcatcatc cagacgaaaa tgatgccgag
     7201 tgccatgctg acaagtaacc agagtttccc caacgcttgc ggcattttac cgatgaccgc
     7261 caccagccac cataatccgc caacggcaaa aaatatggaa ccggcagcaa tctcgttact
     7321 cagaccagaa gctccgattc tattcagcga gttaaacgcc cgcagtggcg atcccaggtg
     7381 catgacagag gcaataaacc cgatgcccat caacagccag aggaaaaaca tgccacgaat
     7441 aatgtgctga cggtctgcgt catttttggc ggcgaaccag ccaattccgc taacaatcag
     7501 cgcgcctact acgcattgac ccaatacggt aaagataacc agtggccatt catgccatcc
     7561 atttcccatt ttacacttcc tccggattag ccagataacc agtggtatcg ccggtcgggc
     7621 ggctgttggc gttaggtttg ataacgatat tgggttttgt gaaatgcgcg cgcggcagtg
     7681 gcgcgacggc agccagagtg ccgtgcttct ggcgcaattc ttcaattgga ccgaactcaa
     7741 gtgcacgcag tgggcaggat tcgacacata tgggttgttt cccctcggcg acgcgcgaat
     7801 aacaaccatc gcacttcgtc atgtgccctt tttcagcatt gtactgtggc gcgccgtacg
     7861 gacaagccat gtggcagtag cggcagccga tacaaacatc ttcatcgacc accacaaagc
     7921 catcttctcg tttgtgcatt gcgccgctgg ggcagacttt ggtacacgcc ggatcatcgc
     7981 agtggttgca cgaaattgag agatagtagg caaagacatt ctgatgccag acgccattat
     8041 cttcctgcca gtcgccgcca gcatattcat aaatacggcg gaaactgact tccgggccaa
     8101 gatctttaaa atctttgcac gccagttcgc aggttttaca gccagtacag cggctggaat
     8161 cgataaaaaa tccatattgt gtggtcatgg gctactcctt aaaccttttc gatctggaca
     8221 agattgctgt gcgacgggtt tccctttgcc agcggtgaag ggcggtgaga ggtcagaata
     8281 ccgttaactg cagtggatag gtcccccgta ggggatcgtt ccaaccgtcg aaacctgggg
     8341 tatacgctgg cagtggatga ataatttcat cttttttcaa ttcccaggta tccgcaattg
     8401 tcgccagtcg ttcagaataa atttcgattt ttcccgaagg tgttttcaac gggtttgcct
     8461 gtggatctac acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa
     8521 agatcccggt cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt
     8581 tcgcatggag atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa
     8641 cgtctggtcc taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa
     8701 attttgctga ggttgcgggt tgggcgagga tcacatagcc catattcccg gcagattcat
     8761 gagagataag gtcttcttgc tctgttggca tcaggtcggg taacaggata tcgcaatact
     8821 tagccgaggc agtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct
     8881 gaagcacctc atgggtgtga ttgatgtcgc catgctgatt gatcaatgtg ttactggcgt
     8941 agcaccataa aaacttgatg gggacatcca gtttttcttt tcctcgaaca ccatcacggg
     9001 tcgcggtcat ttccgtacca tggtcgatgg catctgtcca tgtaaagacg gaaagccgct
     9061 catccgcgtt tctgacgggt tatttccgaa cataacgacc agtttcgtat tggcgatatc
     9121 atccgggctg ttgccatcat tggcaccgaa catataactc attgcggcac tgatctgtgc
     9181 ggtactgtag ctgccatagc gactgagaaa accaccgcaa gagttcatca gacggtacgg
     9241 gacgtttgag ttggtgatgt ttccgccatc tacgcctgtt ccatagagaa catgtacagc
     9301 ctcattgccg taatctttta ggatccgccg aagattatca ctgatggtat ccagggcttc
     9361 gtcccaactt atccgttcaa atttaccttc accgcgcttg ccgacgcgct tcatgggata
     9421 tttcaacctg tcaggatgat tcatccgtcg gcggatagag cgcccgcgta aacatgcccg
     9481 aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca gactccaccc agtacacggt
     9541 gtcatctttc acatgcaaac gtaacagaca gcggctcccg cagttaacgg tgcaggaact
     9601 ccagaccgct ttctcttcta ccggtgcctg tgccgctcgg accatttggg aaaacggcaa
     9661 agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg gaggttttca tcagacttct
     9721 acgactgatt tcagccgaca tgagcgcctc tgtgttatgg attttcatca ttactcactt
     9781 attgcttttc aaacaaaatg tcatgtcaaa atttatggcc gtagtgggtt atattttttc
     9841 gatctcgacc agattagtgt gctgcgggtt tcccttcgcc agtggtgaag ggcgcagagt
     9901 ggttagcgta ttcacacagc cgccgtggtc gattttatcg ccagacatat tggcctcgtg
     9961 ccaggctccc tggcccatag cactaactcc agggagaatg cgtggtgtca ctttggctgg
    10021 tagccgaact tcgccacgat ggttaaacac ccgcaccata tcgccgttgg caatcccacg
    10081 tttctgtgca tctatagggt tgatccacac ttcctgacgg caggctgcct tcaggacatc
    10141 aatgttgccg taggtcgagt gcgtacggga tttgtaatgg aaaccaaaca gttgcagtgg
    10201 gaaggtacta cgttcagggg agtcccaccc ttcaaaggtt gaggcataaa ccggcagtgg
    10261 gcttatcact tcatcttttt ccagttccca ggtacgggca atttccgcca gcttgctgga
    10321 ataaatttca atcttaccgg aaggcgtttt aagtggattt gcctcggggt cgtcacgaaa
    10381 tgctttgtag gcgacaaaat gaccattggg atctttacgc ttatagatac ccattttttt
    10441 taattcgtcg taagacggta acgccggatc tttggcaagc attttggcgt acagatgttg
    10501 taaccattgt tcttgcgtgc gaccttcagt gaacttttga tagacgtcag gtccaagacg
    10561 tttcgcgact tcactcagga tccagtaaat tggtttgcgt tcgaattttt cgctggtgac
    10621 aggctggagg aaaatgagat atcccatgtt accggcgtag tcgttaggaa taatatcttc
    10681 ctgctcaacg gtcattaggt ctggcagcag aatgtcggca tattttgccg atgaggtcat
    10741 aaagttttcg atgaccacaa tcatttcgca tttcgattcg tcctgcagaa tttcatgcgt
    10801 tttgttgatg tcagaatgct gattaaccag ggtatttccc gcgtagttcc agatgaactt
    10861 aatgggcaca tctagtttat ctttgccccg gacgccgtcg cggattgccg tcatttgcgg
    10921 gccatgatcg atagcatctg tccaactgaa gcaggagatt gacgttttga ccggattatc
    10981 cagcaccggc aggcgttcga tggtaatggt gtaggtcgat tcacgcgcac cactatttcc
    11041 gccgctgatg ccgacattgc ccgtcaaaat aggcaacata gcaatagcgc gtgccgtcag
    11101 ctcgccgttt gcctgccgtt gcggccccca gccctggcag atataagcgg gttttgctgt
    11161 gccaatttca cgcgccagtt tgatgatacg gtctaccggg atacctgtaa tttgcgaagc
    11221 ccactgcggc gttttcgctg ttttatcatc accttcacca agaatatagg ctttatagtg
    11281 accatttttg ggtgcatctg cgggtaaggt tttttcgtca tagccgacgc agtatttatc
    11341 gagaaaaggt tgatcaacga gattttcgtt aatcaatacc caggcaatac ccgcaaccag
    11401 cgcggcatcg gtgcccgggc gaataggaag ccattcatct tcacgaccag cagccgtatc
    11461 ggtatatcgc ggatcgataa caatcatttt tgcgttcgat ttctcgcgcg ctttttcaag
    11521 aagataagtg atgccaccgc cgctcatgcg ggtttcagcc gggttgttac caaacatcac
    11581 gaccagcttg ctgttttcaa tatccgtggt gctgttgcca tcattactgc cgtaggtgta
    11641 gggcatggca caggaaattt gcgcagtgct gtaggagcca tactggttta gtgaaccgcc
    11701 gtagcagttc atcaggcgtt tgaccgccga ggctgatgga gaagagcggg tcatattgcc
    11761 gccaacaatc cccgaagagt actgaatata tacagcctca ttgccatatt gttcgacggt
    11821 ttttttcagg ctactggcga tagtatccag ggcttcatcc cagctaatcc gttcgaattt
    11881 gccttcgccg cgtttgccca cgcgtttcat tgggtaattc aggcgatcgg gatgattaat
    11941 acgccgacgg atggagcgac cgcgcaaaca ggcgcgtacc tgatggttgc catacttgtc
    12001 gctgccggtg ttgtcagttt ccacccaggt cacttcatta tctttaacat gcagacgaag
    12061 tgcacagcgg ctaccacagt tgacggaaca ggcaccccag accacttttt cgctggcctg
    12121 ttgtaccgct gctgctgcac tgcgcagggt aaacggcaaa gaaaaaccgc ctgcagccag
    12181 cgccagagaa cctatcgcag tagatttaac gagtgttctg cggctgatgc ccaccatttg
    12241 ttcatttttg gacata
//
LOCUS       DAPQZD010000147        11881 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPQZD010000147 DAPQZD010000000
VERSION     DAPQZD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.149"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..838)
                     /locus_tag="RO454_003872"
     CDS             complement(<1..838)
                     /locus_tag="RO454_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDY0935633.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDSGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKH"
     gene            complement(864..1751)
                     /locus_tag="RO454_003873"
     CDS             complement(864..1751)
                     /locus_tag="RO454_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935634.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            complement(1773..3347)
                     /locus_tag="RO454_003874"
     CDS             complement(1773..3347)
                     /locus_tag="RO454_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0935635.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            complement(3374..4630)
                     /locus_tag="RO454_003875"
     CDS             complement(3374..4630)
                     /locus_tag="RO454_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDY0935636.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            complement(4630..5262)
                     /locus_tag="RO454_003876"
     CDS             complement(4630..5262)
                     /locus_tag="RO454_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDY0935637.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            complement(5458..5724)
                     /locus_tag="RO454_003877"
     CDS             complement(5458..5724)
                     /locus_tag="RO454_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935638.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(5734..6624)
                     /locus_tag="RO454_003878"
     CDS             complement(5734..6624)
                     /locus_tag="RO454_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935639.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(6621..7286)
                     /locus_tag="RO454_003879"
     CDS             complement(6621..7286)
                     /locus_tag="RO454_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDY0935640.1"
                     /translation="MNKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            complement(7283..7951)
                     /locus_tag="RO454_003880"
     CDS             complement(7283..7951)
                     /locus_tag="RO454_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDY0935641.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(7951..8631)
                     /locus_tag="RO454_003881"
     CDS             complement(7951..8631)
                     /locus_tag="RO454_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDY0935642.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            8714..10273
                     /locus_tag="RO454_003882"
     CDS             8714..10273
                     /locus_tag="RO454_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDY0935643.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQMVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKTPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAENGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            10276..10554
                     /locus_tag="RO454_003883"
     CDS             10276..10554
                     /locus_tag="RO454_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935644.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQVKLKEKNIKMSKAEVINTI
                     LEQLTMADFDKVISSVGASAKTREKIMRIYENSNMTKEDLETLLSRLK"
     gene            10623..11045
                     /locus_tag="RO454_003884"
     CDS             10623..11045
                     /locus_tag="RO454_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935645.1"
                     /translation="MKYSLLPLLTLPVLMLTACQSRPISIHDAKPAPQAKVFKYQSAA
                     PATLVVMRDTGFVGAGCDASIFINGETVAKLATGEKATFHLNAGELIVGASLEGAGLC
                     ALNPARQERETTLKNRDTKAFRVFTSNSGDIDILPTTL"
     gene            11050..11574
                     /locus_tag="RO454_003885"
     CDS             11050..11574
                     /locus_tag="RO454_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0935646.1"
                     /translation="MTTKDITYGIQAELWPRDYTNVEKLLMFWRREQIPVRVTLEDGQ
                     AFCMYVYGLMPSRNKVDLCPAPFDKENRIRLPLERISTIESGVVDSIAHDFKGRLTVH
                     PDYVDNRPSRRDFFAICNQAYKANKSIRVYMADGREIEGVSAGADACQVTLRVENGRK
                     IVVLFDWVERILPF"
BASE COUNT         3034 a         2982 c         2647 g         3218 t
ORIGIN      
        1 cgtgcttatt cgccagagag cagtaccact tcacacgggt acgtactgcg tctttgttct
       61 gtacagtacc aatgttttca acaacgatac ctgcgttgtc gccgccatac agacctgtaa
      121 cgccgttctc ttctgacaga tgcaggcagt agatgcttgc tttgccagaa tcggtcggga
      181 tgaagtcgtt gatgatgaac ggaacgccgt tatgacacag catcggacga ccgaagttct
      241 ccatcatgat ttcagacggg cctacgttta ctgtacgcag cagcgcacgg taagcacgca
      301 ggtgctctga acgcatcatg atgcagtcag caccaagatc tttcaccgca tcgaccagtt
      361 cgtcgaacat agagaaagtc atagatgcgc cggcgatatc gatcttctga tctgcgtgca
      421 tcaggcgtgg aatgccgtca aacgccttgt tgttggagct ggagtcaccc aaaatcaggt
      481 tgcgacggaa cgcacgagcc agacctttaa ctttctgacg aacctgaata gccaactggt
      541 tgttggtgtc ggccatagtg gtcgccagga atttatcaac gtctacgtca ccagccagaa
      601 tacgcagttt cgcaacgcat tcttcgaagg ttgctgcacc ttcagggatg gtgtcgttaa
      661 cgtcgatgaa ggtagcttca ctcagcgttt tttcacggtt gtacagatat gcctttgaat
      721 taattttcat aaaaggcagg atggcaaaca ggtcatcgcg atcgatgata gtttcgatca
      781 caccctgttc aagttcgttg ttagacagct tttcagcttc ttcacgcagt aatggcatct
      841 atcaattccc tttgatttag atgttactta agtccaattt tccccagacc ggaggccaac
      901 ttatccatag tcgacttgtt cttcggttgg gatattgtgt aggtcgattt ggaatgtgag
      961 cctacaccct gcttggcttc gctacgcatc aatgcgtcag cttccggatc tgcccgcaca
     1021 atgcgttcaa tcgcggattc aaacggcaac ggcttacctt caccgtcaac cagaacagct
     1081 cgttctttgt gacctgccgg tttgtcataa cccactacgc taccgtcttc acccacttca
     1141 aaatgagaac cgtagatcac gcgggctttt gccggggtca tcagaacttt gtcacgtagg
     1201 aagagagagt tactgaagga agcgcccacg gtcatctcga ctaattgaga tttcagtgat
     1261 gcgttttcac tttccagtgc tgcataacgt tcgtcacgct gtgccagctc tgcctggtgt
     1321 gcttcgatca tctgtttttt aacagcatcg aactcaccac gacgctccag ttcagcttgc
     1381 tccgcctcac gacgtgcgtt ttctgcggcc tgttcagctt ctaaaagctg gcgagcacga
     1441 gccggatcga tatcaccgta ctgagcaagc tgatcggcca atgtgcgctc tttctctttg
     1501 cgcttcatgt tctccttcag cagttcagca ccagctttct tggttttacg aagttcggcc
     1561 agtaactctt cctgagtcat gccagcgtat tcgtcatcgc ccttcggctg ctctttttgt
     1621 tcaccctgtt tgtcaggatc ttgtgtactc tgctcattat cagcagctac accgccagcg
     1681 cctccacgct catgcgcttc agcgacatcc atcagaccac gacgggccaa aagcatttgc
     1741 cacagattca taaaaattcc ttttgttact tatcactcgt tctcttgagt agatgagtcc
     1801 ccattccctc ggggttgatc ttgcccgctt tcttgggctg caccacgatc ataagtaagt
     1861 actgacttat tttcaagggt gtttagatca ttttttggag gaaaattcaa gagatcttta
     1921 tcaaattcct ttttcatcgc ctcagtaatg ttcgggaaaa tcttctcaat aagcatttcc
     1981 atctggtgac gacgtacaga gtccggtgcc tgaagtaatg acagtttctc ggcaacagaa
     2041 aattcatcag taaggccacg aatatcgaaa ctttctggat acgcaattaa agagtggtct
     2101 tcgtccagat ctacccccat ccatttcgca accagtagca tgatttggcg ttcagccctt
     2161 tccagacgct ctgcttttgt gacaagcaag ctatttacac gctggaagtc atacattttt
     2221 gcggcaccag atgaattatc gattccctgt gcgttgtcct gcttcgttcg ctcaccagct
     2281 acaccaactg aatggtagat ttcgttaatc accgtcttaa tcgtagtgat gatcatctga
     2341 gcttgtttcg ggtctggtga cagataaaac ggctggtttc caccttcaga atcgtaggtg
     2401 aagactcgct ttgtgcccat ttcaagcact ttagtgtggt tttcatcacc aggtaaaagc
     2461 gactgtaccg gtatagccaa ctggctaaat gtctgatcct gaataatggc atcaaggttt
     2521 gacagatagt ttgcaaccgc acgatcaaga taagcgatat catcgattaa cgatgggctg
     2581 aaatacggtg attcactttc tccaatacaa tcaacaggaa acacaggaac tacgccgaga
     2641 ttatgctcac cgctatcctc taaaataact tttgcctgac ggcgacctgc tccaccagcg
     2701 cccttcttca cttcctcacg gaacagatac cactcgtttt gtgtccacag acgataacgt
     2761 tggtattcct gacctgtaga agtaaaagga tctgcgtcat cacgagcgac ttccacaatt
     2821 aacgcccata acatattccc gtcgtcgtcc catgccacat ccagcatttg ctgaggtgaa
     2881 atccagtagg cgtaggcacg agcatctttc tttttctcgt cagctactga ctcaacatca
     2941 ccactcattg tgctatcaac aacaacccag atacggccgt agattgacga ttgcaaatca
     3001 atggcggcca taaatgagtc aatagaggca ttctggcgag tcgcacgttt ccagaaattg
     3061 cggatctgct ctggtgcctc ttcgatattt ctatgaatgt cttctttaaa gagatatttg
     3121 ttgatgaggt ttaccacctc acgagtgtgg ttgaagcgat aagcacgctc aactcgctcc
     3181 ttaaactcct gatctccctc tttaaagtaa cgaaagatat tgtctgtaaa ccaaccacgc
     3241 ccgccagcgt aagtgctggc gaggaagtcc caatgttctt ttttcttttc gtattccggg
     3301 tggcgtcgcg ccaccagatc cttaatttgt ttgtcgttca attccatttg aataacctta
     3361 gataaccact tacctatcga gaaccaccaa gaataacacg gtttttgact ggatacctac
     3421 gatgtactgg atagcccaat gcgtctgcac tatgctcaat gccacctgtc ttatccatat
     3481 ctcgtgttcc aggcttgtag atgacttttt ccagtgaatc aatcaaatgc ttgcacttcg
     3541 gatcgatata cagccggata tctccagatg cggacatcag cattcggtta acagcattaa
     3601 cacgatccgc aattggcggg tgctttttcg aataatcgac acgtaagaat cccttctctt
     3661 tgaatatgtc tacgtcagac tccccacgag catgttggcg ataagcacct gccggatctg
     3721 gaaataccgt tacctgtgat ttccagcgcc agaagcgtct ctcaagctca tcacaaactt
     3781 ctgccgtgtt agaggaaaag agcaccaatt catcaattgc ccatagctca ccatttggct
     3841 gaggttgcaa aattactgaa gacatcgggt caatgttgaa gtcctgccct acccatatag
     3901 gcaaccgagg gttgaactga agcggtttta catgcacgtt acgatcgaac gggtagtaaa
     3961 cgcgcccgga catgttctca aagctggcca ggtactcctg agcgaacgat ttggggtcca
     4021 tatcgttctt agcagcttca atttccgccg taggtacaaa cggagaatcg gcagttacaa
     4081 actgccagct cttccactgt ccctttcttt gcagctctac gttctggcct atagtccaca
     4141 gtttgtgaaa ttccgagaag ccttttggcg taccaatgat cagtgcaccg ccgcgtgtcg
     4201 atgaaagagt aggtcgtaac accttgtacc aggtgtcagc cttcatatcc tgaaattcat
     4261 caagcactac aaaatgcaac gctacgccgc gcagagtgtc aggcttatca gcacctttga
     4321 gggcgatctc cgaaccgttc ttcaacacga ttgtcatcgt ggtgtcgttc tttttcctaa
     4381 cccacttacg aggcagaact tcctgtagat cgtcccacaa aatctggcga gccatctggt
     4441 aagtaggagc gacataccag accctttgct ttttttcctt tgccgccgca cgaataatag
     4501 tggagatcga caaccgggat tttccccagc gtcgaccagc acaaacaact ttaaatcgat
     4561 gcggagactg gaagactttc atctgtccag aatgcagttg cacaagactg agcgaggacg
     4621 ggattgccat tattcgtcct ccccttcact tccatcatct gtcgcatcaa attcgcttag
     4681 agcttcttct tccaacgtct caagcaattc gtcatcgatg atttcaggct cgtcgtcttc
     4741 ctgacgtaat ttcgccacct gggaaggcgt aagctcacca aataccaggt tcggaatttc
     4801 ttcctcgtca ttttccgcat gatccatgcc caatgctttg gacgaaactt caaagcattt
     4861 tgcaagggta ttactggctc tctgtaagct cttgagagaa tcctcaatcg cccctaaagg
     4921 cttaccctca cgtttggccg tagtgacttc gaccatcacc atctgcccca acgcatacgc
     4981 ccagccgtca taacgtgtac gacgttcttc tatcttttcc gcacgggctt tagcgcgaag
     5041 ctctgcgtca gatttaagag actcacgaac catcttccca acagagtccg cgcctttctc
     5101 taatcctcgc tttttgaaat gtctggagag tgtttcacga cgaatgccgt actcttcctc
     5161 cagctttgag agtgtatatt cgcctgacgt ccatttggct tcagcttcgg cccactccgc
     5221 tggtgtcagg cgagttttta cctcgtcttt ttcgaccgtc atagatccct ctaaaacaca
     5281 cagagcgcgt ccatgcgctc taaaacaact tgtttactgc atctgctaac caacttgttt
     5341 tctggggtgt ttaattaggt ctggcatgtc ttacgaagcc tgcttccgta tatatttaat
     5401 aagttactta ttatttatat atacagatgc aggtctttaa ataagctccc agaccgatta
     5461 catcaccagt aacttcgctt tggctcgacc taaagtggtt aacccaagag ttcggcggtg
     5521 atagcgattg tcactgcgtt ggcgcgtatg ccctttctcc accagcccct tttttatcag
     5581 agcgcgaatt gagaactgga tactttgctt ggttgtcttg tacggcaaaa cttcaagcaa
     5641 ttcgtccagg tcaagtagat gacctcgctc ataaccgaga ttgagcgttt tgatgatgtc
     5701 cttttgttta tcggttaacg tcatggcaaa tccttatgcc ggtaaagcaa tttctaacgg
     5761 tttatccaaa ggttgtttgt cgaatgccag cagtggcagc gtgtcaggca gtcgacggcc
     5821 aaagtcaggg tttcggtaca caccatacaa cggagacgta aagctcaggt tgtgaatgtc
     5881 cttgagcagc ttcacaatgc tggcctcgtc caccagactg tcggcaatgt cctgaatcgt
     5941 cgtgccacga ttccgcccag cttttgccag ggaactgttc ttgtggtagt ccgccaccag
     6001 atcacgcagt gcacggcgac gacgagactc gctcattgcg aacaactctt tgacgatcgc
     6061 ctcgttatca cccgggtcgg aacggaaatg gcgctggaat acacgcagtg cactttcata
     6121 gctcttcggt cgctcagggc ggatgaactt aaaccctgct ttcatggcga agggattgta
     6181 tttgctcatc gaggactgga tctcaatgat tggccggtca tgcatcctgc taaccaggtt
     6241 aatcattcga taagagacgc ctacgccacg atactgagta tccacaactg agcgactgat
     6301 caccgcaaag ttgttattca cgtaccgacc ccagtactgg ttagccacgg tggtattagt
     6361 ggttggtttc aacttaggaa acatgcggtg gcgaggtgcc agcagtagtt tcgggtaagc
     6421 cataaccacg acgcccacca gacggtcatc aagttcgcag cgatagtaag ttggcgcgaa
     6481 aggtttgccg tctgttttgt agtgaagcga cttaagagcg tgccagtcct ctacagttcc
     6541 ctttgtgaca gtcattcgct ccagaaagtc cagatgacgc ggaaactctt cagggcggta
     6601 gcgtttaatg atgatgtccg tcataaatga acctcatcat gactaccatt gtgatattcc
     6661 accttcacgc gttctttgta gtgcttggtg atctgcatat ccgggcgcag cgcgttcttc
     6721 aggtcttcgt gagtcgtcgc caccattacc gtcgcaccaa cctttcgagc ggcacgctgg
     6781 aggttagatg ccacaacctg agcggttaca cggtctagaa cagcaccgaa ctcgtcagca
     6841 gcccacactt tagcgcctga ctcaatcagt ttggcaatct tgagacgata tttctgaccg
     6901 tctgacattt cagaaggctt gcgaacaaac agatatgcat cgttcagacc agccatagat
     6961 aacaacccaa gcgcatcgct ggtcgttttg cccaactgat cgatgacgtt aacctcatta
     7021 tcgaaggtaa aatcatcgat ggaggctaca gacagccctt catccttcat ctgtcgttgc
     7081 aactcgcgca gcacaacgga tttgccggaa ccggattggc cggtgatgta caccacatcg
     7141 ccctgcttca cttccagctc cagattgtcg taaagcgtcc actctttttc gtccaggcca
     7201 agcccgaacg actcagcgat ttccaacgtg cgcgtggttt tatttacgcg tgtctgaaac
     7261 gatacgttga tgatgtattt gttcatgcag ccatctcccc ggaagagatc ttctccgcat
     7321 atgccacaaa tgcgtctacc ccgctttctc ccgtcatttc ttccatgtgg gcaagcaaat
     7381 caccaacaac aatggcagag ccagcaggaa gcgttttaaa gcccaatacg tcgacaacac
     7441 gtacttcttc cgctgcaact tcacgactga tctcggtgtg ttcatccttc tgtcgtttag
     7501 tttcttcgcc aagatcgata actagcgagt cggtgtccat ttcttctgtc atgctgccaa
     7561 cgagaacatt caactcacgc tcttcaaagc cgaaaacctc gatatcgtcc agaacaagcg
     7621 actcaagctc tttctgtagc ttaattgcat cgtaatcaat gctggcaagt cggttatctt
     7681 caaggcgctt cgcacgaacc tcgtcatcac tgagatcatc gcgaacaata accggtacgc
     7741 gctcaagtcc agcaaaaatt gcagcctcac ggcggccgtg gccagtaata attacgtcgt
     7801 tcttatcgac cgtaattggc tggtcaaatc cgcgcttttt aatggctgcg gctagatctc
     7861 ggatctgctg ttcatcatgc tttttggcat tcatctcata gggaataaga tctgccgggt
     7921 cgcgatatac gatttcaaac tttttggtca ttacatacgc tccttgtagt agtcgaccag
     7981 ccacaccaga gcctcaccag cgttctccat ttcattaccg gtgttaatag cctgctcttt
     8041 gatgatgttt tttatggttt ctgcaacacg atctgacgca tcgaaagtta ctttgaagcg
     8101 catggtctga tgttccgcac ccacacgttc ggttttctct cgtttgtcgg tatcgacagg
     8161 ctcatcacta ccacgagaca acgcctccag tgcttcaagg tcgattgccg cctcttttgc
     8221 taaaaccatc gagatttcgt cgtcatacgg ggcgatttca gacagctgat agtcaagttc
     8281 tgactgaatt tcttcaatga agcgttgcaa tgcgatttgg tcgtcttcac cgtatcgctc
     8341 gttgtccacc agtgacatct gtttagctac gacatcgcta attttgccca cagaaagcac
     8401 cggaaccgtt gaaattcctt gctcaatagc agcacgccag cgatgttcgc cgccgaggat
     8461 ttcaaaaaat ccatcttcaa gttcacgagc caaaattggc ttaaaaaagc ccaatttttc
     8521 gatagaacct ttcagttttt caaaattctg cgcaccaacc gaattggtgt tccaggtatt
     8581 cgggcgaagg ttggcaacat caacctgcaa aatcgtgatt tttacatcca ttttattgct
     8641 acaatccact aagtaatcac ttacttatta taatagccaa ataacataca aaaggcacta
     8701 aggaaagagg tttatgactg ttcggattgt atctaacgca gttaatgcgc ttatttctgg
     8761 cgcagatgac aaggtaaagc aaatggtgca acagatgttg agctacgaag tcgagactgg
     8821 cgactggaag ggcacaagca cgatgttcaa ctggagtaaa aactcgttcc ctgctggctt
     8881 tgccaagcct gtagcggcga acttgaacaa ggcgggcatc aaatgtgttc atatccgcaa
     8941 agacaaaacc ccggcgcttg gtaaaccaaa tcctgcggtt aacccattcc catacaatcc
     9001 tgattatgcg tatcaggatc agactgtgga aacactggtt cgagagggaa tgatgattgc
     9061 gcagatcgct actggtggcg ggaaatctaa cgttgcctgc aaagcagctg cacgtatcgg
     9121 tcgaatgaca ttatttttaa caacccgctc tgttctgatg tttcaaatgg ccgaaaactt
     9181 ccagagatcc atcgactacc gcgccgaaaa tggcgaaccg tggttaaaag accaaaaggt
     9241 tggagtcatt ggctcgggtg agttccaggt atcacgacat atcaacgtcg ctacagttca
     9301 aactcttgca agtttcctcg aagaaccacc acgcgatgca acaccagata agaaaagcta
     9361 ccacctcaaa cgtcgggagt tggtgaaacg cttcctttca agtgtctctc ttcttattct
     9421 ggaagaggcg catgagtctt caggctcaaa tttctatgac atcgccagat tatgtgtgaa
     9481 cgcagactat cgtctggcgc ttacagccac gccgttcatg aaggattcga cggaagccaa
     9541 catgcgcctg atggcggtgg ccgggcgaat tgaaattaaa gtcacagaaa agtacctgat
     9601 tgatcgaggc attctggcaa agccgtactt cctttatcat aaagttgcct acaagccaga
     9661 cgaggccaga atcaaggccg aacttgccaa caaacacctc aattttagag ttggtatgag
     9721 caccgcctac caaaaggctt atcagttggg gatcgtgtat aatttgggac gtaacgaggc
     9781 cattgttcgc gaagcattgc tctataagca acattctctc aattgtatga ctctggttcg
     9841 tcttaaacgc cacgggcaaa tcctgatgga aatgatgaaa gagtccggcc ttagagttga
     9901 cttcatctat ggggaatcta accaggcgac aaggcaagca aagctgaaca gtttagcgtc
     9961 tggagaaata gatgttttaa taggctcgac tattctggat gtcggtgttg atgtgccaag
    10021 cgttggtgcg gtcattcttg ctggtggtgg gaaagcagaa gttgaaatga ggcagcgtgt
    10081 cggtcgtggc ttacgagcca aaaaaaatca ggcaaacgtg tgttttatca ctgatttcat
    10141 tgacattagc aacaaatacc tgttgtctca ctcttatgag cgaaaacaca tcatcgacac
    10201 cacacctggc tttgcagaag gtgtattgcc tattgatgga gcattcgatt ttggagttct
    10261 gaaacgagat tagttatgag cgaaaagaaa acaacttatt gtcaggtagc attgtctgat
    10321 aaggccaatg acaaacttgg aaagtttcaa gtgaaactaa aagaaaaaaa tatcaaaatg
    10381 tctaaggctg aagtcataaa taccattctg gaacaattga caatggccga ctttgacaag
    10441 gttatatctt ctgtcggggc ttccgctaag actcgtgaga aaatcatgcg tatctatgag
    10501 aactctaata tgacaaagga agatctcgaa acgctattaa gcagattaaa ataatcacgc
    10561 tatcaattag ggagacaaag atgaagtctc ccatactgtt cactttctgt tctaaaacta
    10621 tcatgaaata ctcgcttctc cctctactaa cgttaccagt gttgatgctt accgcgtgcc
    10681 aatcacgccc aatatcaatc catgacgcca aaccagcacc gcaggccaaa gtgttcaagt
    10741 atcaaagcgc agcgccagct acgttggtgg taatgagaga cacaggtttt gtaggagctg
    10801 gatgtgatgc ttccattttc atcaacggcg aaactgtcgc aaaactggct acaggcgaaa
    10861 aagcgacttt ccatttaaat gccggagaat taattgtggg tgcatctctt gaaggtgcag
    10921 gtttatgtgc tctaaacccc gctcgtcaag agcgagaaac gactctgaag aatagagaca
    10981 caaaagcgtt cagggtgttc accagcaatt ctggtgacat cgacattctg ccaacaactc
    11041 tgtgatgaca tgacaaccaa agatattacc tacggtatcc aagctgaact ctggccgcga
    11101 gattacacta acgttgaaaa actactgatg ttctggcgca gagaacaaat tcctgtaagg
    11161 gtcactctcg aagatggcca ggcgttttgc atgtacgttt atggcctcat gccatctcgt
    11221 aacaaagttg acctttgccc agcccctttt gacaaagaaa atcgtataag gctcccactt
    11281 gaacgcatta gtacaattga atcaggtgtg gttgacagta tcgctcacga tttcaaaggt
    11341 cgactaacag ttcaccctga ttatgtcgat aatcggccat cacgccgcga tttttttgca
    11401 atttgcaacc aagcctacaa agcaaacaaa tctataaggg tgtacatggc ggatggccgt
    11461 gaaattgagg gggtgtcagc aggcgcagat gcttgtcagg ttacactacg tgtcgagaac
    11521 ggtagaaaaa tagttgtttt gttcgattgg gttgaacgaa ttttgccttt ttgagttatg
    11581 aaatcgatat tgttaccacc attattttta ctttgctcag ttgcggcaaa tgcaatggac
    11641 tataagcctg tcattcagtc gctgatgaat gacgtgtgct caacgtctca gaatgtatca
    11701 gtttgcatgt atcaattttc ggcagccgta aaagcaggaa aagcgatagg tgagaatgtg
    11761 gaactgtgta agaaagtggc aaatgaagaa cgggcaatgt tggattgcga atctagcgag
    11821 tcatcggcac agttcgttga tgcgctattt gacaccaatc gtaaggctgt agagtctgtt
    11881 c
//
LOCUS       DAPQZD010000148        11699 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPQZD010000148 DAPQZD010000000
VERSION     DAPQZD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.150"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            25..1011
                     /locus_tag="RO454_003886"
     CDS             25..1011
                     /locus_tag="RO454_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935647.1"
                     /translation="MYHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1163..2391
                     /locus_tag="RO454_003887"
     CDS             join(1163..1478,1478..2391)
                     /locus_tag="RO454_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0935648.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVIPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRADDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRVYRTMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQWASDGLSDNRCLEI"
     gene            2891..4981
                     /gene="iha"
                     /locus_tag="RO454_003888"
     CDS             2891..4981
                     /gene="iha"
                     /locus_tag="RO454_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HDY0935649.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(5199..5358)
                     /locus_tag="RO454_003889"
                     /pseudo
     CDS             complement(5199..5358)
                     /locus_tag="RO454_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5843..6085
                     /locus_tag="RO454_003890"
     CDS             5843..6085
                     /locus_tag="RO454_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935650.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            6376..6735
                     /locus_tag="RO454_003891"
     CDS             6376..6735
                     /locus_tag="RO454_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935651.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHEV"
     gene            6739..6954
                     /locus_tag="RO454_003892"
     CDS             6739..6954
                     /locus_tag="RO454_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935652.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(7532..7854)
                     /locus_tag="RO454_003893"
                     /pseudo
     CDS             complement(7532..7854)
                     /locus_tag="RO454_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7874..8383
                     /locus_tag="RO454_003894"
                     /pseudo
     CDS             7874..8383
                     /locus_tag="RO454_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            complement(8417..9949)
                     /locus_tag="RO454_003895"
                     /pseudo
     CDS             complement(8417..9949)
                     /locus_tag="RO454_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10365..10787)
                     /locus_tag="RO454_003896"
                     /pseudo
     CDS             complement(10365..10787)
                     /locus_tag="RO454_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10832..>11699)
                     /locus_tag="RO454_003897"
     CDS             complement(10832..>11699)
                     /locus_tag="RO454_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HDY0935653.1"
                     /translation="KYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDE
                     RLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRAT
                     TNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYA
                     MGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRK
                     GNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISP
                     AAFREKYHQMAA"
BASE COUNT         2995 a         2726 c         2944 g         3034 t
ORIGIN      
        1 aatctattat gtaaaggggg aatgatgtat catcatgatt ttaatgaaaa aataggtttc
       61 tggtatgtga tagccctcgc aggacagtct aatggtatgg cctacggtga aggtattccc
      121 ctgccagata cactcgacaa acccgagtcc cgtgtaaaac aactggctcg tcgaaaaaca
      181 atcacaccag gaggaaaaga gtgtaaattc aatgagatta ttccggcaga tcactgcctg
      241 catgatgttc aggatatgag tggatatcac catccggctg ctgatttgca taaaggggaa
      301 tatggttgtg tcggacaggg attgcatata gctaaaaagt tactgccata catacctgag
      361 caagcaggga tattactggt tccttgttgc agaggaggtg ccgcctttac tgttggagct
      421 gaaggtatgt atgtacctga tacaggggca acagctgatg caatgcggtg gggcaccgga
      481 acagcactct atgaggactt agtagcccgg gttaaggtag ctctggaata taacagaaag
      541 aataaactgc tatctgtctg ctggatgcag ggggagtttg atcttatgtc accagattat
      601 gaaaagcatc ctgacttgtt ttatcagatg gtaacttctt tcaggtctga gttatctgaa
      661 tactcatctc agtgtgttgg taattcgtca gaaagagttc catggttgtg tggtgataca
      721 acctggtact ggaaggaatc ttatcaaaaa gaatatgatt ttatatatgg acattacaga
      781 caacggactg atgatgaaat acattttctc agctttcagg atagtaaccg acatgaatta
      841 actaatgagc cagaagaaga tgccgatgat ttatctgtcg ggtatctggg gtcatcatgg
      901 aggacagaat taagctggac aacgtcacag agaagtacac attttaattc aatggccaga
      961 aggggagtta ttgctgaatg ttatgcgcaa aaaataagaa attatttgta gacaagttca
     1021 ggatatatct acagctctga catgattctc tgagagacat gacggagtgg ttagactggc
     1081 cccctgaatc tccagacaac caatatcact taaataagtg atagtcttaa tactagtttt
     1141 tagactagtc attggagaac agatgattga tgtcttaggg ccggagaaac gcagacggcg
     1201 tactacacag gaaaagatcg ctatcgttca gcagagcttt gaaccgggaa tgacggtctc
     1261 ccttgttgcc cggcaacatg gtgtggcagc cagccagcta tttctctggc gtaagcaata
     1321 ccaggaggga agtcttactg ctgtggctgc cggagaacag gtcattcctg cctctgaact
     1381 tgctgccgcc atgaagcaga ttaaagaact ccagcgtctg ctcggcaaaa aaacgatgga
     1441 aaatgaactc cttaaagaag ccgttgaata tgggcgagca aaaaagtgga tagcgcacgc
     1501 gcccttattg cccggggatg gggagtaagc ttcgtcagcc gttgtctccg ggtgtcgcgt
     1561 gcgcagttgc acgtcattct cagacgagcc gatgactgga aggacggccg ccgcagccgt
     1621 cacacggatg atacggatgt gcttcgccgt atacaccatg ttatcggaga gctacccaca
     1681 tatggttatc gtcgggtatg ggcgctgctt cgcagacaaa cagaacttga tggtatgcct
     1741 gcgatcaatg ccaaacgtgt ttaccggacc atgcgccaga atgcgctgtt gcttgagcga
     1801 aaacccgctg taccgccatc gaaacgggca cataccggca gagtggctgt gaaagaaagt
     1861 aatcagcgat ggtgctctga cgggtttgag ttccgctgtg ataacggaga aaaactgcgg
     1921 gtcacgttcg cgctggactg ctgtgaccgt gaggcactgc actgggcggt cacaacgggt
     1981 ggcttcgaca gtgaaacagt acaggacgtc atgctgggag cagtggaacg ccgctttggc
     2041 agcgagcttc cggcgtctcc agtggagtgg ctgacggata atggttcatg ctaccgggcg
     2101 aatgaaacac gtcagttcgc caggatgttg ggacttgaac cgaagaacac ggcagtgcgg
     2161 agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg tgactacata
     2221 agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga ggcgttcgag
     2281 cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg ggaatatctg
     2341 cggcagtggg ccagtgatgg gttaagtgat aacaggtgtc tggaaatata ggggcaaatc
     2401 cattgtcagc gccaacaatg tgggctgaca tgataaagct gtctaggaaa tcgttcgcct
     2461 cctcagcgga caatccaaat ggtgattgtc tctgttaaac gtttattttg aaggtcgact
     2521 gaataaggtg atgacgctgt agaatttttc acgtgccaca gaattttgaa cgctttctct
     2581 tacaatattt caatgtttct atcagtattc gccggaagaa gtcatcgacc aaatcatccc
     2641 agtcgtctcg caccactgac cattcatggt tgacatgtgg tggaaatccg cttctacagt
     2701 aaccattttt tattcgcaaa accgaacacg ccatacggat agctgttaac tggcatgccg
     2761 aggcagtcgt tatttatatt tagttttgtc aataatcttt attttttgta aaaggcaaat
     2821 ataaattatt ctcattattg tttgtattta tgtattgtct tgccggttaa catgatcgga
     2881 gattagtaat atgcgaataa ccactctggc ttccgtagtc attccctgtc tcggattttc
     2941 agccagcagc atagctgctg cagaggatgt gatgattgtc tctgcatccg gctatgagaa
     3001 aaagctgact aacgcagccg ccagtgtttc tgtgattagc caggaggaat tgcagtccag
     3061 ccagtaccac gatctggcgg aggctctgag atcagtagag ggtgtggatg ttgaaagtgg
     3121 tacgggtaaa accggtgggc tggaaatcag catccgagga atgccagcca gttacacgct
     3181 gatactgatt gatggtgttc gtcagggcgg aagcagtgac gtgactccca acggtttttc
     3241 tgccatgaat accgggttca tgccccctct ggccgccatt gagcgtattg aggttatcag
     3301 ggggccgatg tccacactgt atggctctga tgcgatgggc ggtgtggtga atatcattac
     3361 cagaaagaat gcagacaaat ggctctcttc cgtcaatgca gggctgaatc tgcaggaaag
     3421 caacaaatgg ggtaacagca gccagtttaa tttctggagc agtggtcccc ttgtggatga
     3481 ttctgtcagc ctgcaggtac gcggtagcac acaacagcgt cagggctcat cggtcacatc
     3541 actgagcgat acagcagcca cgcgtattcc ttatcccacg gagtcacaga attataatct
     3601 tggtgcacgt cttgactgga aggcgtcggc gcaggatgtg ctctggtttg atatggatac
     3661 cacccggcag cgttatgata accgggatgg gcaactgggg agtctgacgg ggggatatga
     3721 ccggaccctg cgctatgagc gaaacaaaat ttcagctggc tatgatcata ctttcacctt
     3781 cggaacatgg aaatcgtatc tgaactggaa cgagacagaa aataaaggtc gtgagctcgt
     3841 acgcagtgta ctgaagcgcg acaaatgggg gcttgccggt cagccgcggg agcttaagga
     3901 atcgaacctt atcctgaatt cattactgct tacccctctg ggagaatctc atctggttac
     3961 ggtggggggc gagtttcaga gctcgtccat gaaagacgga gttgtccttg ccagcacagg
     4021 tgaaactttc cggcagaaaa gctggtcggt atttgctgag gatgagtggc atctcacgga
     4081 tgcacttgcg ctgactgtgg gcagccgcta tgaacatcat gagcaattcg ggggacactt
     4141 cagtccgcgt gcatatctgg tctgggatgt ggcagatgcc tggacgctga aaggcggtgt
     4201 gaccacggga tataaggcac ccagaatggg gcagctacat aaagggatta gtggtgtatc
     4261 cgggcaggga aaaacaaatc tacttggtaa ccccgacctg aagccggaag agagcgtcag
     4321 ttatgaggct ggggtgtatt acgataaccc cgccggtctg aatgccaatg tcacaggttt
     4381 tatgactgac ttctccaaca agattgtctc ttattccata aatgataaca ccaatagcta
     4441 tgtaaacagc ggaaaggccc ggttgcatgg tgtggaattt gccggcacat tgccgctgtg
     4501 gtcagaggat gtcacgctgt cactgaatta cacctggacc cgaagtgaac aacgtgatgg
     4561 tgataacaaa ggtgcgccgc tgagttatac ccctgaacac atggtgaatg cgaaactgaa
     4621 ctggcagatc accgaagagg tggcatcatg gctgggtgcc cgttatcgcg ggaaaacacc
     4681 acgtttcacc cagaattatt cgtcactgag cgctgtacag aagaaagtgt atgatgagaa
     4741 aggagaatac ctgaaagcct ggacggtggt ggatgcaggt ctgtcgtgga agatgacgga
     4801 tgccctgacg ctgaatgctg cggtgaataa cctgctcaac aaggattaca gtgacgtgag
     4861 cctgtacagt gccggtaaga gtacgctgta tgccggtgat tacttccaga cgggatcatc
     4921 aacaacagga tatgtgatac ctgagcgaaa ttactggatg tcgctgaact atcagttctg
     4981 ataataacaa aacgctatca ctgacggtag aatacgttgc cactgtaact cctggcggaa
     5041 cagtggcaac gtattaggtt aagtgcattt ccgatccgct aatgagattt cgttaccaac
     5101 aactaatatc gtcacaggaa atgcacggat tatttttaac ttatcattta catacttgtc
     5161 cagagtgtaa gcgcaccgtg aaggacgtgg ggtaaaaatt agtgtacaga gagagtgacg
     5221 ttccagggca acaactcttt catgcggttg gcaggccagg tgttgattac actgatcacg
     5281 tggcgtagcc acgtttccgg ctcgattccg ttaagttttg cagctaccga tcaggctgta
     5341 catcactgcc gcactatcgc tcgtcatctc aaagtccggt ctcgtcagca ggaaggtatc
     5401 attctctccc gccatttttc cgggggcccg gtcagataag tccctttgtc tatcgctgac
     5461 tcctggctca taacccggtt agcagaatgc agggtcacca cctcgccacg accgaatccg
     5521 gataagtcaa ttgcaccttc tcaatcgcca ttttgtcaca attaagtttg ataaatcatg
     5581 tcacctctgc actgaatctg ctccagtgcc tcattggtct ccgtccaccc ctttaaactg
     5641 ccggataaga ctgctgcaca gcgccttccc cggaatcaaa tcactttccc tgtccgccac
     5701 aatccgcttc atgccacctc gtattttgtt tcagagaccg gaatctgcca gaaagtgact
     5761 acgcgtgcac atatcggttt tctgggttac atgcatcgtg ttattttctg aaatgcaaaa
     5821 taataaaaaa caaggacaca taatgaagga tgaaatacac agtaccagca tcaacccgga
     5881 tgttgttgag cagtttctgg cccttatgaa cacacattgt aaatcctcag aagaggcgct
     5941 cgccatgctg agtacgcttt gttacaacct gatggtgaca tgcggtatcc attccatgga
     6001 cagccagatg ctcaatgggc atgggatatc catgcaactt caggggattg atattgccga
     6061 aaggccaggt aagaatattc actaacctga acagggtctg aaaacgctgt cttttcaggc
     6121 ctctgcaggc aaaaactgat gacaacaaaa tttctgcgtt tgtaccccac cgatgaaaaa
     6181 gacaaaaact cacaccgggc ttctagccag taaagataaa acccgacgtg tcagcctcta
     6241 cgaaacgcca acggcatggt gtatcagggg gcaggagtgc tacagcaaaa gtacaggacg
     6301 ccgctgtggc agccacgaca gtctgagcag actgcgcctg gacagcatta agcctgttga
     6361 atgagtggtg gcaccatgaa agataacagc acaaccgcca ccccagaagc ctggaaaatg
     6421 cgttttctgg ccaacgggac atccggcaca tggttttaca ctgacgacat tacagtcact
     6481 gacgcgtggc ttcgcgcgca cccggatgat gtggaggtta tccctctctg ccggatacca
     6541 ccccgtcagc attcccatga acaacatatt gaagaactcc gtgcagccct ggcccggatg
     6601 ctgcatatgc atcatatgat gctggaaacc acgcattaca gggaaaacat caccgatgcc
     6661 cgttgtatcc gtgaaatgca tgaagctcca ttacaggcgc tcaatattct ggccagatac
     6721 ccgcacgagg tgtgacgcat gtggctactg attttctggc tttcatctcc ctttaatgtc
     6781 tcttccggtg atgcccggca gcccacactg gcggtgcaca gccaggcatt tcataccgaa
     6841 cgggcctgtc gcgatgcatt gcacgccatt aaaaaggaga atgttggcga attttctctg
     6901 cggggcgttt gtgtttctga cggaaatacg aaggaggaag ctgagagctc atgacggata
     6961 ttactgacag acatacactt cgccgtatgt cctggtcgga actcttcacg gcagcacagg
     7021 aagcggaatt tcagcgtgat tacgagcgtg ccagaatagt gtggtcattc gccttacatg
     7081 ttgcaacaac cacgattaac aaaaatctgg ccatagctca tattcgacgc tgcaatactt
     7141 tgttgcataa atcaaaaaca gtacccggaa acaacacggg gggccggtca gtgtgcctga
     7201 gaccccaaca tccccggaga tgactgacat tccggactga cgcagttgct gcatatctgt
     7261 ggcaatacgt tgtatccagc cagacaggtt atcccgcgta gtgacaatta acccgctttc
     7321 ctgcaccagc ttcagccggg ccgtcgggga ttttaccagc cggaaatatc agtaaaacaa
     7381 catatcatta tgccatggtg tacgcggcca gtgtacgctt acttaaggaa tgagggataa
     7441 attggatatt attacttcac tttgcttttt tatgagattc atcacataag tggggtttta
     7501 ctgttaaata atctgaattt atttgatatc cgtaaagcag atgtctcagt tcgctgtttc
     7561 caacttttgt tattcctctg gggcgaatgc tgtttccgct ggaatattca cctggcacca
     7621 gtcccagaaa agccatgagc tgttcaggat tggaaaatcg cgaaaaatca cccagttcag
     7681 caaccagggt tattgcgatt agttgaccaa cacctttaag agcctgcaac tggcagacca
     7741 gattgcacag agatcactca ggtaacaggc gagatatttc ctgatcgagt tgaagtatgc
     7801 gatcttcaat ctgctccaga ttctgacaat aatgttgaaa gcaatctgtt gagatagcag
     7861 accaaatgat tgagcgagtg ttagtgatga caaacgtatc caggtacttc ttatctcatg
     7921 gtgttttggt ggatttcttg aggcggcagc cggatacggt acagcggtag caatccccat
     7981 tggtatactg attgctctgg gctttaaccc actaaaagca gcgattgcat cactggttgc
     8041 taatacagtc cctacagcgt ttggggcagt aggtatacct gtctctgtac ttgctgaaca
     8101 agttaattta cctgtaacca cactcagcgg gacaattatt cttcaactgg cactgttcaa
     8161 cattctgctt cctttcgtca tcgtcgcgat tatcggtggt ggtatgaaga ctatacgagg
     8221 cgttggacta attaccctga tgtgtggtgt cagtacatta attccccagt atctggtggc
     8281 tgttaatctt ggcgcggaac tcccggcttt cgcgggtagt cttgtcagtc tgatagtaat
     8341 agtctttatg gcgaaaaacc gtaaagaaaa aaccgcccct gaattgggga tgcggacgat
     8401 ttcctagact gctttattat gccagcccac attgttggcg gtacatttca gggctgactg
     8461 cacccagcga taattttata cgccgctcgt tataccatct gatgtaagca tctacatgct
     8521 gcatgagctt ttctggcgtg atgcccgacc atttacgccc ataatacatt tccgttttca
     8581 gtctgccgaa aaaaccttcg catgcggcat tatcaggtga acatccttta cgggacattg
     8641 agcgaataag acctgctgca ttcactcttt ccagccagcc tggccagcga taatgcccac
     8701 ctctgtcact gtgtatcacc ggtcgttcgc cagcatttaa cgtttcgaca gcgctatcca
     8761 gcatagtgtt gaccagctca gcatcggggc gtgtactgag agaccagcta ataacttttc
     8821 catcgaagca gtccaccacc ggtgacagcc agactttacc tgcagggagt tggaactccg
     8881 tgatatctgt cagccatttc tgattaggtt gctacgcctt aaaatctctg gcgataaggt
     8941 tatccggagc cggtccgatt tctccgcaat atgagctgta gcgacgacga cggttacggc
     9001 tgacgacaag ctgttcctcc accataagtc tgcgtacaac cttttctgat agccgccccc
     9061 cctcatgcct gagcatcgca tgcaggcgac gatagccata acactggtaa ttactgttaa
     9121 atatatctgt cagcatcgta cgtatcgtgg cgtatttatc accggctttc agtgcagccc
     9181 ggtgataaaa ataactgcta cgggtaaggc ccagaacatg aagtaattct gtcaggggat
     9241 atgtttgcct cagggcatca gcgatctttg ttttttctct gttgttcagg tgactgatac
     9301 tgatgcccgg gtcttttttt atgatttcct ccgccttttt cagaatatcc agctccatct
     9361 gccggcggcg tatttcctga ttcagtcggg cgacttcctc ccgcaacgca tcgcgttctg
     9421 cctccaggga aggttcgtta tgtttacgca tagtctggta agcactgttg ccgataattt
     9481 catctttcta tttatacaat accgcacgac tgacaccaat acgcctggcg atctcactgg
     9541 ctgatacttg tcgtgtacag agtgccatga cagcctgacg tttgacttct ggttcaaagg
     9601 gcgcaaccgg gttggcctta ctggtaaata tacgtcgtct gtcgggataa agttcattaa
     9661 cccagcgagc gagtacatca gtgcagggat aacccagggc tctgctggta aacgccaggc
     9721 agcagccgtg gttaagataa tgttcaacgg caacctgttt ttgctcatcg gagtaacggt
     9781 gcttatgacg aatggattct tttgcgccac cacctgcttc ccatgagcgg atccaacgcc
     9841 gcagatttct tttcgaaggg tagcccagtt cgcgtacgac gggagccagc ttcttaccat
     9901 atttgaaata aagctcaatg gcgcggattt tttcttcttc agtaaacatg ttttatctcc
     9961 tgagagtcca ggaaatcgtc cgcatccccc aacgagctgc tgtttaaatt cgggaggata
    10021 attagggcag ccttttcgcc tgccgggagt cacatttttc tgcatatctg acactttggt
    10081 tcccactact tatttggtgg acaccacttt gtctaattcg tcagattctg accagacggt
    10141 tcaggctgta cgcttacagt tgttcaggag taatagtcca aagtatcggg aattttccag
    10201 acaatcctga taatacgctt atgaacatta caacacgctc atgcggaacc tggatggggg
    10261 aaggaacggt gaagctgtta gcgaatgact ggcgtttgta taaacgccgg tcatgatgaa
    10321 tgatatttca gaatatacct cgggctaaaa taccgggaca gagatcaggc atgattaatc
    10381 tgcagcattt ttaataacag aagtactgag cttactttac ctttgatgat ggaatagtta
    10441 tttccgtcgg tacttatttt tccctgaaat aatgtcacgg atgtttcttc ctgctgatcc
    10501 tgcaggcgca gtcgtaaggt catattttta atgagctgag atccgggtgg caaggggtat
    10561 ttgctgtcag atactgggcg actgagtctg atgtggcgcg tatacagccc gttttcgtac
    10621 agggtggcct ctgttatggt gtcagtgacc agcatgtggg agacaatgct gtattctttt
    10681 ttaaagcacc caccagaaac ggtgattgta atgataatgg tcttctcgat cgagatgtaa
    10741 atacccgttt cgttatactg tccgtatcgg aaccatgaga actcagtttg aggtgtactg
    10801 gcaatagcgg acacaaccat ttgttctttt tttaagcagc catctgatga tatttttccc
    10861 tgaaggctgc cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa
    10921 tctcaatgta ttcccgtatt actgagatgg cttcatcccg gttattaaaa cgatagtggc
    10981 tcaggctctc atttttcagc gttccccaga agctttccat cggagcgttg tcgtaacagt
    11041 tacctttacg cgacattgat gttttcagac caaactgctc ctgtatgacc cggtaatcgt
    11101 atgcgcagta ctgtgaacct cgatcagagt ggtggattag cccggcaggt gggcgctggc
    11161 tcctgagcgc cataaacagg gctttacctg tcagctcttt tgtcatgcgc tctcccatgg
    11221 cgtagccgac aatttcgcac gtataaacat ctttgatgcc agcgaggtac aaccatccct
    11281 cctgtgtggc aacatacgtc aggtccgcca cccagacctg atttggtgct gtaggagcga
    11341 acgtctggtt cagcagattt ggcgcaactg gcagattgtg gttcgagttc gtagtcgctc
    11401 tgaacttgcg tttctgctta cagcgtagcc ttagctcctt acgaagacgt gccagtcggt
    11461 cacgaccaac gatgatgcca ttctctgcca gctccgtctg gagccgccgg gttccatatg
    11521 tttcgcgagt gcggatatgt gccaccttaa tctccagttt tagccgctca tcactttgtt
    11581 ttctgtctga gggttcatgc tgtacccagt tgtaataacc gctcctggat acaccaaata
    11641 cctgacacat cgcttcaatg ggaaattgtt gtcgccattg ttcgattaac gcgtatttt
//
LOCUS       DAPQZD010000149        11645 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPQZD010000149 DAPQZD010000000
VERSION     DAPQZD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.151"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            173..466
                     /locus_tag="RO454_003898"
     CDS             173..466
                     /locus_tag="RO454_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935654.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(507..1697)
                     /gene="nepI"
                     /locus_tag="RO454_003899"
     CDS             complement(507..1697)
                     /gene="nepI"
                     /locus_tag="RO454_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDY0935655.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            1931..2284
                     /locus_tag="RO454_003900"
     CDS             1931..2284
                     /locus_tag="RO454_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDY0935656.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2268..2591
                     /locus_tag="RO454_003901"
     CDS             2268..2591
                     /locus_tag="RO454_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDY0935657.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2707..3159)
                     /locus_tag="RO454_003902"
     CDS             complement(2707..3159)
                     /locus_tag="RO454_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDY0935658.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3212..4546)
                     /gene="adeQ"
                     /locus_tag="RO454_003903"
                     /gene_synonym="yicO"
     CDS             complement(3212..4546)
                     /gene="adeQ"
                     /locus_tag="RO454_003903"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDY0935659.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            4721..6487
                     /gene="adeD"
                     /locus_tag="RO454_003904"
     CDS             4721..6487
                     /gene="adeD"
                     /locus_tag="RO454_003904"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDY0935660.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(6534..7925)
                     /gene="uhpT"
                     /locus_tag="RO454_003905"
     CDS             complement(6534..7925)
                     /gene="uhpT"
                     /locus_tag="RO454_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDY0935661.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(8063..9382)
                     /gene="uhpC"
                     /locus_tag="RO454_003906"
     CDS             complement(8063..9382)
                     /gene="uhpC"
                     /locus_tag="RO454_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDY0935662.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(9392..10894)
                     /gene="uhpB"
                     /locus_tag="RO454_003907"
     CDS             complement(9392..10894)
                     /gene="uhpB"
                     /locus_tag="RO454_003907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDY0935663.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(10894..11484)
                     /gene="uhpA"
                     /locus_tag="RO454_003908"
     CDS             complement(10894..11484)
                     /gene="uhpA"
                     /locus_tag="RO454_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDY0935664.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         3216 a         3169 c         2865 g         2395 t
ORIGIN      
        1 gcagtttaaa aaacgaaacg ctatcgataa gaataaaaag gaataaaagt ggaatataaa
       61 gtctggcact tcttactgac cacgcaaaca cctttcgtac agcatgatga gagcggcaaa
      121 agcaaactac acttatgctt cattcgatgc acatttgcag aaggttgccg ctatgaagcc
      181 aacgatgcta ctcatgatta ccatattcct gatttttcca gccatttctc aggccgagtc
      241 gccattcagt tccctacagt cggcaaaaga gaaaaccacg gtattgcaag atttacgtaa
      301 aatttgtacc ccgcaggcat cgttatcaga tgaagcgtgg gaaaagttaa tgttgtctga
      361 tgaaagcaat aaacaacaca tacgcgaagc catcgtagcg atggaacgta ataatcagag
      421 caattattgg gaagcgttgg gtaaggtaga atgcccggat atgtaagaac ggcaacaacc
      481 actgaccaac gcttctttac ctgatatcag gatttcttca ttttcacctt tgcagtcacc
      541 aacaatgcag tcagcaacat caatgtgccg gacaacatca gcggcgaagt cagaccaatg
      601 ttatccaacg cataaccgcc gattgccgca ccgcaggtat tggcaagctg aataacggcc
      661 acctgaatag atccggcttt ttctgcctga tcggccagtg agcgagtgat ccacgttgac
      721 cagccaacgg gaattaaagc aaaggtcagc ccccagataa tcgccacacc agtagcaaca
      781 attttatcgc ttccccacag cgtcagtacc agcgcgctca gggcgagcac aaacggtgcg
      841 cccgctaagg ccagtttcac cgaacgtttg agaataaacg acgaaagcga tgtcccgaca
      901 aagctggcga taccaaaact cagcagcacc agggttaagc catcaacacc gaatcccgcc
      961 agattcatat acactgggcg aatataagtg aaaaaagcaa actgtccggc aaaggacata
     1021 aagatggcga tcatcccggc catcactccc ggacgttgta ataagcggaa agtattttgt
     1081 ttctgatgcg aaggttcgcc tggcagcgaa ggcagtgatt tgataatcca gaaaatacac
     1141 agcacgccca tcaccgccgc cgcattaaag acattgcgcc aaccgataag ctcgcctaaa
     1201 aaactgccca acggcgcggc aatcaccagc gcaatagaaa ccgcgccgaa gatcaccgac
     1261 agcgccttcg gcaccgtacg cggcggtacc agacgcatgg tcagcgacgc cgacatcgcc
     1321 cagaatccgc ccagcgccag ccccagacag gcgcgaccga ttaacaacag actgaatgag
     1381 ttggcaaagg aaaccagcaa acaggaaagc gtcagcaaaa cggcgaacag aatgacaacg
     1441 tagcggcggt cagtggcctg aatggtctgg gtaataaaca aactggcaaa catcgccaca
     1501 aaagcggtta cggtcaccga ttgcccggca accccttccg aaatgcctaa atcctgggcc
     1561 attggcgtca acaaactgac gggcaaaaac tcaacgataa taagacaggc aacgcaaaat
     1621 gccaccgaga aaacggctga ccagttcggt cgggtgatgg catccgcgcc gcggttttca
     1681 gcaataaatt cactcatggt gttacccgtg gcatgtttca gagggaaagc cgaccacttt
     1741 aacattgaaa tgtgtgacgt atttaacgtt tttgcaactt ttacggtgtt gtgggaaaag
     1801 ttcgaggctg ctcgaagaaa taaaaatcat agggcaaggt gatatgcatt ttcgcgatta
     1861 ggttgggaat aagggataaa gtattgcact tggtgatata ctttaataac aggatctcaa
     1921 cgatatgcgc gtgtttaaaa ccaaatggtt tgcaagggaa gccaggtctc atgcaataac
     1981 agatgaagag ttgtgccggg ccattttaga aacggaacaa ggtaaagctg atgtacttgg
     2041 tggtggcgtt tttaaaaagc gccttcaccg aaatcgtgag cgtgcaatta ttctggcgaa
     2101 agggggaagc aattggtttt acacgttttt atatgcaaaa caggatatgt cgaacattaa
     2161 tagtcaggaa ctggctgggt ttcgtgaact tgcaaagcat tatgccttcc tgacgaaagc
     2221 ccaactgaca gcaatgatta acactaaaga actgacagag atctgctatg actgcaaaaa
     2281 ctaaatttaa aagtcctgca tttgaagcta ttcacagcgc agcggctgga ttgtccagag
     2341 tcgatgctat ttctgccgaa acgatgcgta cttttgacaa agcctgcctg accagcgtgc
     2401 aagacttaca acctgttgag ataaaagcac tgcgggaaaa attaaaagtg agtcagcccg
     2461 tcttcgctca ctatctcaat accagcgttt caactgtaca aaaatgggaa agtggcgcta
     2521 aacgccccaa tggcatttca ttaaaactgc tcagcattgt gcaaaaacac gggctggagg
     2581 tgctgctgta aatagaacgt agataatgcg ttcacctact gtcaattccg ataccggatg
     2641 cgacgtacac gccttatcca cccaccaggt caggcgcggt ctgtaggctg ataagacgaa
     2701 gcggttttaa tgcagccagt gaacgccctc ggcaggctga aagaacgcgt tatagcgcat
     2761 ctgaaaagca ttgatatcct gcatttcagg ttcggtttcg cgcagaaaac ccagcgccag
     2821 tctgacctga gcatcggtaa tcggcgcggc aaggcgggct ttttgtaaaa tccgatgcca
     2881 ttgcagcagg ttctgttcgc taccatcgac aatgacaatt tgccagatga gcaacacctg
     2941 cgcggcgttt tgcaggtgcg actcatccgg gcgatgcaaa taacgtaaaa attcgtcacc
     3001 ggctgacttt cccatttgat cgaccatcga ctccacgggt acgacatcga tgttcagccg
     3061 atcgctgaga cggcgaatcg cttttctggc cccggatgtc agcacccgaa aaccaaccac
     3121 aaacactacc gccagcgtgg cgagcattat ccagatcatg agttctcctg cgtaatcaga
     3181 actcatgata tctggaaacc ctcgccagtt actaatccac cagaataatc ttcagtgcaa
     3241 acagagctgc gaccaccacc acacacaggt tcagatcgcg ccagcgcccg gtgcacactt
     3301 tcatgatgca gtacgacata aagcctagtg caatcccttc ggtgatcgag aacgtaaagg
     3361 gcatcatcac cgtggtaata aacgcaggca ccgattcggt aaaatcatcc cagttaacgc
     3421 gtgccaggct cgatgtcatc aatacgccaa caaagattaa cgctccggcg gttgcgtaag
     3481 gaggaaccat cgccaccagc ggtgagaaga acataaccag cagaaacata acgccgacaa
     3541 caaccgcagt cagccccgtg cgaccaccga cagccacacc tgaagtactt tcaatatagg
     3601 cggtaacaga cgaggtaccg ataaacgcac ccgccaccga actgatgcta tcaacataca
     3661 gcgccttatt catattgggg aatttaccgt tactatctat taagccagct ttatcagtca
     3721 caccaattaa tgtccctgat gaatcaaata agttgatcag cataaaggag aaaatgatac
     3781 cggcgagttc aagtgataac gcaccgctca aatccacttc accaatgacg ccgctaatat
     3841 caggcggaat ggaatagaca ccgctaaaat gaacatcacc gaaaaataat ccacagcaag
     3901 acgtcaccac aatagaaaca agcaccgcgg catgaaaatg acgtgatgac aacacagtta
     3961 taataaaaaa ccctaaaata cctaacaaca cgccgtgaga acttaaatcg ccaatcatca
     4021 ccagggtgtc tttattggcg acaataacgc cagtattttt taatcccatt aaggcgataa
     4081 ataatccgat tccgctggtg ataccaatac gtaaacttaa tggaatgttg gagatcatcc
     4141 agtaccggat acgaaacagc gtgagcaaaa atagcccaac tgcgccccag aatatcgcgc
     4201 ccatcccggt ctgccaggag atacccatcg cccccacgac cacgaaggca aagaaggcgt
     4261 tcaggcccat tgccggagcc agtgccacgg gtaagttagc aaataccccc atcgcaatac
     4321 tgccgatacc ggcaatcaaa caggtggtaa caaacaccac ttttgggtcc atttgtgccg
     4381 cgccgaggat ttgcgggttc acaaaaacga tgtacaccat ggttaaaaaa gtggtcatcc
     4441 ccgcaatcaa ttctgtgcgg acggtggtcc catgctgagg tagtttaaat aatcgcgaaa
     4501 gcgtccctga ttcattactc acgtaatcgg tattgtcatt attcattttt ttgtccattg
     4561 gaggagattt aatcccttgc ttttgtaaaa caaatgacaa caacggttca gtgataatta
     4621 tcacatttca attgcacatt aatggatatt ctttaataat ctcgcgacgt ttctttatga
     4681 taaataataa tcaaattgat aaaatcaaaa tgagaaaaat atgaataatt ctattaacca
     4741 taaatttcat cacattagcc gggctgaata ccaggaattg ttagccgttt cccgtggaga
     4801 cgctgttgcc gattatatta ttgataatgt ctctattctc gacctgatca atggcggaga
     4861 aatttccggc ccaattgtga ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc
     4921 tgatgctccg gctttgcagc ggattgatgc ccgcggcgca acggcggtgc cagggtttat
     4981 tgatgctcac ctgcatattg aatccagcat gatgacgccg gtcacctttg aaactgctac
     5041 cctgccgcgc ggcctgacga ccgttatttg cgaccctcat gaaatcgtca acgtgatggg
     5101 agaagccgga ttcgcctggt ttgcccgctg tgccgaacag gcaaggcaaa accagtactt
     5161 acaggtcagc tcttgcgtac ccgccctgga aggctgcgat gttaatggtg ccagttttac
     5221 ccttgaacag atgctcgcct ggcgggacca tccgcaggtt accggccttg cagaaatgat
     5281 ggactaccct ggcgtaatta gcgggcagaa tgcgctgctc gataaactgg acgcatttcg
     5341 ccacctgaca ctggacggtc actgcccggg tttgggtggt aaagaactta acgcctatat
     5401 tgctgcgggt attgaaaact gccacgaaag ttatcagttg gaagaaggac gccggaaatt
     5461 gcaactcggc atgtcattga tgatccgtga agggtccgca gcccgcaatc tcaacgcgct
     5521 ggcaccgttg atcaacgaat ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa
     5581 cccgtgggag atcgcccatg aaggacacat cgatgcctta attcgccgcc tgatcgaaca
     5641 acacaatgta ccgctgcatg tggcatatcg cgtagccagc tggtcgacgg cgcgccattt
     5701 tggtctgaat cacctcggct tactggcacc cggcaagcag gccgatatcg tcctgttgag
     5761 cgatgcgcgt aaggtcacgg tgcagcaggt actggtgaaa ggcgagccga tcgatgcaca
     5821 aaccttacag gcggaagagt cggcgagact ggcacaatcc gccccgccat atggcaatac
     5881 cattgatcgc cagccagttt ccgccagtga ctttgccctg caatttaccc ccggaaaacg
     5941 ctatcgcgtt attgaggtaa tccataacga actgatcacg cactcccgct ccagcgtcta
     6001 cagcgaaaac ggttttgatc gcgatgatgt gtgctttatt gccgttcttg agcgttacgg
     6061 acaacgactg gctccggcct gtggtttgct cggcggcttt ggcctgaatg aaggtgcgct
     6121 ggcggcgacg gtcagccatg acagccataa tattgtggtg atcggtcgta gcgcagaaga
     6181 gatggcgctg gcggtcaatc aggtgattca ggatggcggc gggctgtgcg tggtccgtaa
     6241 cggccaggta caaagtcatc taccgttgcc cattgccggg ctaatgagca ccgacacggc
     6301 gcagtcactg gcggagcaaa ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt
     6361 accggatgag ccgtttattc agatggcgtt tctttccctg ccagtgatcc ccgcgctgaa
     6421 actaaccagt caggggctgt ttgatggtga gaagtttgcc ttcactacgc tggaagtcac
     6481 ggaataataa aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     6541 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat caccgccact
     6601 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc
     6661 cagccggtaa ggccaaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     6721 aagctgtcac caatcaggta agcgaaggtg cctttaatac catcggcagc gccaatcgct
     6781 tttttaggta caaagccaac agccgccacg ccaatcaaca gttgcgggcc aaagaccagg
     6841 aaacccaacg caaagagaga agccaggtag atatattggt tgctggcgtg ttgatacaca
     6901 ccgagcgtgg cgataatcag cgccagcgcg atacaggcca ccaggccacg gcgaccgttc
     6961 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     7021 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc atacacagtt
     7081 gaccactggt cgataccaat acgcaccaca tagaggaaaa tgttggcgaa gcacagcagc
     7141 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     7201 gattctgtct ctttatcctc ttcactgatc tcctcgccaa acagctcttc agctttgccg
     7261 aggccataag attccgggga gtcgctgccg taacgcagac cgataaaacc aacaatcagc
     7321 gcgataatcg acgggaaaat aaacatgccg atgacatgac catcgaacag gtaatttgcg
     7381 ccgaacagcg ccacacctgc tgcgcctgca ccaccaaggt tgtgagaaat gttccagaaa
     7441 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcat
     7501 gaaccgccgg tactctggaa aaagccgctc aaggcgtaga aggcaatcat caggaacagg
     7561 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatggc agacaggatc
     7621 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtaaga aaccagcgtt
     7681 ttacccacgc cataagtgat ggagaagccc aggccgatca tccccagctg cgtcatgctc
     7741 aacccgtagg tcgagatcat gtcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     7801 atcgtaaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     7861 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taagaaagcc
     7921 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     7981 aaatcaggcc tgagatagcg tccaggcaaa acctggaaaa tttcttagtt ttgccggata
     8041 tgaagtgaaa aggtgagatg catcacgctt ctcgcggtgt ctgggcgttc aaaaagggca
     8101 gtaacagcag tgcggaaatc ccggcggcga tggcgataac cacaaaaaat ccgctccagt
     8161 gccaggtatc gagtactttc gccagcggcc aaccagcaag cgacgcacca agataagcaa
     8221 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     8281 ccatgccgat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     8341 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     8401 cggcggcgaa aatcaaattc atcggcccgc ggttgccgtt aaacagtttg tccgaacccc
     8461 aacccgctac cagcgcaccg ataaatccgc ccagttcaaa cattgtcact gccgtattcg
     8521 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
     8581 ccgcccgaac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     8641 gcaacacata tttggtgagg atctctttac gcgtcagccc tgccccttct tgttgttgag
     8701 caatctccag tgcgtcgtgc cgccattcac caactgccgg taaacctaac gcctgcgggc
     8761 gatcgcgcag ccgccagcag agaaaaatcc ccacgacgat cgccatacaa cccgcaatca
     8821 tcatcccggc acgccagccg taatgcagcg cgctcgctgc catcacaatg ggaatgagtg
     8881 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgag
     8941 aataccaggc ggttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     9001 tcaacaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga
     9061 taatgcccgt ggcaataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
     9121 caatgccgga gacaaacttc gacacaccgt aggtaatgta aaacagggtc gctaacaggc
     9181 caatatcgct acggctgagc acgccgttag caaggatttc tggtacggcg gagttaaaac
     9241 ttttacgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     9301 gccagtagcg atagcgggca tcaatttcat gtttatcagt cattaatggc gcatcggcag
     9361 gcgctttcag aaacggcaac atcatcgaac cttagacata acgttgaggt agagaaacgc
     9421 tgacacgcgt gccgtgcagg caggagatgg tcaatgtgcc acccagcgcc gttacgcgct
     9481 cgcgcattcc ggtaaggcca aaaccgtgtt gcccggaatc tggcggtaaa ccgctgccat
     9541 cgtcttcaat caccagcatt aaccgctcat catgctgcca gccttgcagc gtgaccgcgc
     9601 tggcatcggc atgtttcaca atgttgttca acccttcctg gcagacgcga aatagcgtca
     9661 cgcgctggtt ttcgcttaac gctgattcat cgattcgcca ttcgagatga ctgacaatgc
     9721 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
     9781 catccagctg gcgcggacgt aaccgcccta acaagcggcg caccgcgtcg taaacgccca
     9841 acgacaactg ttcgatgagc tgcccgctct gtttcacgct ggcgttatcc gccgcgagcc
     9901 gctggacaat gccagcctga gtacgaatag cagtgatggt ctgaccgata tcatcgtgca
     9961 gctctcgcgc cacatcgcgg cgcacgctct cttcggtttc cagcaaccgt tcagcgagat
    10021 gctgattgcg cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc
    10081 cagcgcccag taacaaccct gtcagacttt gcaccagcag cgagagcaat aaatccaccg
    10141 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaat gtcgcaatca
    10201 gcgctccttg ccaaccatag tgccaggcca gcgcgataat cggcagcgcc agacagaatg
    10261 gcgtaaagcg cgacagttcg tctggcaatc ccaactggag ccagagactg ataacaaaca
    10321 gcagcaagta ccagaccaga tgtcgcccgc gccagttgat tggctgagaa accagtgacg
    10381 ggccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
    10441 ccagcgtcag gccgccagtt aaagtcagca gcagcgcatt ccacgactct ttgccgtgcc
    10501 aaagccaggg caacgactgc aacaacgctg ccgccgttaa cgccgcaccc tgtaacagca
    10561 aggtgcgcca gtcacgttga tggcgatagc gcgagatcag cgccacgggc aataacgtca
    10621 gtaaactacc gatcatcaat aaggagaaat gggttaaacc gaccgcctgc attagccagt
    10681 aaatcagcag ccactccgca cccagtagca ccggccagta tccgcgcggg cattgcagca
    10741 ttagccctag ccgcaggcca aacggaaata acagcaccgc catatcaggg cgctcaacca
    10801 ggtgcaggct gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
    10861 taacggtaat taaacgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
    10921 cagttcaacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
    10981 tgttttcggc gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
    11041 tttttctgcc acctggcgtt cgcgtttggt tagcgggtcc tggcgaccag atgccagttt
    11101 aatggcaata tccggcgtca gataacagcc gccagtggca accgtatgca ccgcagcaat
    11161 gagttcatcc gggctacagc gtttggagag aaagccgcgc gcccccgcgt taagcgcctg
    11221 ctcaaccagc gccggactgt cgtgaacgga gagcataatt gtcgccatgc ctttcggcag
    11281 ctggcttaga agctccagac cggagatatc aggcatggag atatcgcaaa tacacacctg
    11341 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
    11401 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga taaggtgatc
    11461 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaataaaac gcgcttacct
    11521 taacgataag cgcgatgttg ttcaagcctt gagcggttac tgccaaaagc tgcgctcttg
    11581 ttgcttgtgg aaagaagtga tgccttcaag gacttgtata tcgactaacc attaaaaatg
    11641 ctggc
//
LOCUS       DAPQZD010000150        11601 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPQZD010000150 DAPQZD010000000
VERSION     DAPQZD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.152"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            272..1042
                     /locus_tag="RO454_003909"
     CDS             272..1042
                     /locus_tag="RO454_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDY0935665.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1033..1506
                     /locus_tag="RO454_003910"
     CDS             1033..1506
                     /locus_tag="RO454_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDY0935666.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1613..2152
                     /gene="dnaT"
                     /locus_tag="RO454_003911"
     CDS             1613..2152
                     /gene="dnaT"
                     /locus_tag="RO454_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDY0935667.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2155..2892
                     /gene="dnaC"
                     /locus_tag="RO454_003912"
     CDS             2155..2892
                     /gene="dnaC"
                     /locus_tag="RO454_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDY0935668.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2941..3435
                     /locus_tag="RO454_003913"
     CDS             2941..3435
                     /locus_tag="RO454_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDY0935669.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3689..5980
                     /gene="opgB"
                     /locus_tag="RO454_003914"
     CDS             3689..5980
                     /gene="opgB"
                     /locus_tag="RO454_003914"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDY0935670.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLTWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6120..7142)
                     /gene="lgoD"
                     /locus_tag="RO454_003915"
     CDS             complement(6120..7142)
                     /gene="lgoD"
                     /locus_tag="RO454_003915"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDY0935671.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEVVGLGKNVADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7281..8195
                     /locus_tag="RO454_003916"
     CDS             7281..8195
                     /locus_tag="RO454_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDY0935672.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFEQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENMRYAH"
     gene            8412..9773
                     /locus_tag="RO454_003917"
     CDS             8412..9773
                     /locus_tag="RO454_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935673.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9822..11486)
                     /gene="tsr"
                     /locus_tag="RO454_003918"
     CDS             complement(9822..11486)
                     /gene="tsr"
                     /locus_tag="RO454_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDY0935674.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLHDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVMPAAPRKMAVADSGENWETF"
BASE COUNT         2819 a         3066 c         2829 g         2887 t
ORIGIN      
        1 ttaacttgtt aacactaaaa aacgcgccca gctatctatt atcctgaccc gtaaaggcta
       61 aaacaataaa ttcattatag tgataatatt catgtgatag tttgtttgcg gcgagagata
      121 attcgctttt tatcaccgag cttgtcccga taaccaggat taatcagcgc aattttgatc
      181 ttaacctacc tcaaaacaac cgtgcttcat gatacaaatg gattctcatt atctgaacaa
      241 tacgcagcac gtctatgaca aagggcgagt tatgcaaact gagcaacagc gagccgtaac
      301 gcggctttgt atccagtgtg gattatttct tttgcaacat ggtgcggaaa gcgcgttggt
      361 tgatgagctt tcctcacgac tgggtcgggc gttgggaatg gacagcgtcg aaagttctat
      421 ctcttcgaac gccatagtgc tgacaactat taaagatggg caatgcctga catcgacacg
      481 aaaaaatcac gatcgcggca ttaatatgca tgtggtgact gaagtccagc acattgtgat
      541 tcttgcggag catcatctgc tggattacaa aggcgtagag aaacgattta gccaaattca
      601 gccattacgt tacccaagat ggctggtagc cttaatggtt ggcctttctt gcgcctgttt
      661 ctgtaaactc aataacggtg gctgggatgg tgccgtcatc accttctttg ccagtacgac
      721 cgcgatgtat atccgccagc tgctggcaca acgtcatctt catccacaga tcaacttttg
      781 ccttaccgct ttcgccgcca ccaccatttc cggattgctt ttgcaactcc ccactttcag
      841 cgatacaccc accattgcga tggccgccag cgttctgctg ctggtgccgg gctttccgtt
      901 gattaatgcc gtcgccgata tgtttaaagg ccacatcaat accggactgg cacgctgggc
      961 gatcgccagt ctgctgacgc tggctacctg cgtcggcgtg gtgatggcac tgacgatttg
     1021 ggggctacgc ggatgggtgt gatcgagttt ctgtttgcat tggcgcagga tatgatcctc
     1081 gccgccattc cagcggtcgg ctttgcgatg gtgttcaacg ttcccgtgcg ggcgttacgc
     1141 tggtgtgcgc tgcttggcgc gattggccat ggttcacgaa tgatcttaat gaccagcggg
     1201 ctgaatattg agtggtcaac ttttatggct tctatgctgg tcggcaccat tggtattcaa
     1261 tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg tggcggccgt tatccctatg
     1321 ttcccgggca tatcggctta taccgcaatg atttcggcgg taaaaatcag ccagttaggt
     1381 tacagcgaac cgttgatgat taccctgtta accaactttc ttacagcttc atcgattgtt
     1441 ggtgcgttat ccatcggtct ttccattcct ggattatggt tgtaccgcaa gcgccctcgc
     1501 gtataaaatt gcctcgctta agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1561 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     1621 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     1681 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     1741 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     1801 gggaagcgat gtcgctctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     1861 tgtccccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg actttatccg
     1921 cctggcggcg ctatggggcg tggcgctaag agagccggtg accaccgaag aactggcctc
     1981 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2041 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2101 gaatacggtc agcgaacctg acagccaaat tccaccagga ttcagagggt aacgatgaaa
     2161 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2221 ttcaaaacgg gtgaagagct actggcctgg cagaaagaac aaggggcgat ccgctccgcc
     2281 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2341 cgtccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2401 aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg acggcaacat cgccagcttc
     2461 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2521 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     2581 aaagatacct tcaggaatag cggtaccagc gaagaacaac tgcttaacga tctgagcaac
     2641 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     2701 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     2761 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     2821 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg tgtaacaggt
     2881 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     2941 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3001 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3061 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3121 agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg cattctgcaa
     3181 tactgcgcga agcaaaagct ggcttcggta accgatgccg aaaacatcaa gaaccaggtg
     3241 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggatggc
     3301 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaacg
     3361 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3421 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3481 tctcctgccc ttttccctgc tcctggacgg ttttacccct gttacacggg aagcgatcgc
     3541 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     3601 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     3661 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     3721 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     3781 attaacagtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     3841 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     3901 tggcgtcagc aaatacatcc tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     3961 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4021 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4081 aacggaactg gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa
     4141 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct atggcgaaag
     4201 tctcgagcga acctattttg ataacgaggc tttcccggat ctcacgcctg aactgggcgc
     4261 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac
     4321 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4381 cgcctccgcc tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4441 aaactcgggt tatcagaact atttcgtgca gggcgcaaat ctgcgttttg ccggtaaaga
     4501 tgtgttcctg aaatcgcacg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4561 ggtagccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     4621 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     4681 gacagtcgat acccatcacc cggatggttt tatttctcgt acctgtaacc gcaaaaaata
     4741 tgattttgac ggtaaaccga atcagtcatt cagcgcggta agttgcagcc aggagaatat
     4801 cgcgacgttt atcaacaaaa tcaaagcgtc accgtggttt aaagatactg tcatcgtcgt
     4861 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     4921 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     4981 gcgtaacacg atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct
     5041 cggacttggt cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa
     5101 agagaaaaca ttgacgtgga agccagatat catccgcctg tggaaattcc ctaaagagat
     5161 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5221 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata
     5281 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5341 tgaccgttgc tataagatgg cacaactctg ggctccggaa ctggcacttt ccaccgactg
     5401 gtgtgtctcg caggggcagc ttggcggtca gcaaattgtc cagcatgttg acaaaacaac
     5461 atggaagagc aagacggcat ttaaagatac ggtcatcgac atggcgcgtt acaaaggcaa
     5521 tgtcgatacg ctgaagattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     5581 taacgtcgcc ggtgcgccag aagaggtgaa acagtttagc gggatttccc gtccggagtc
     5641 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     5701 gctgccgaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     5761 tcctattccg gtacgcgtag gcaatgaaga acaaactctt gtgctgggca atgaagtgac
     5821 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     5881 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     5941 cggcatggtg gaaattaaag ttgtggaacg tgaagggtaa ttcctgactg atgtgtaggt
     6001 cgcataaggt gcgacctaca caccttctcc ggcctacaaa acatcattga gaaacagaaa
     6061 aaaaacagcg cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat
     6121 cagaaagtaa ttacgccttt aattaactca cgattgttaa tcacatcgcg ctcgtaggtt
     6181 tctgccagcg tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct
     6241 tccgccataa gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc
     6301 atcgtcgttt cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacagg
     6361 ccgacaaata ccaccgtgcc accgtgacga atcagattca cggtgttatt catcgcatgt
     6421 tgattacctg tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc
     6481 tgcgcgtcaa aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca
     6541 tgttcacggc gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg
     6601 attgccgccg cgcccagacc gattggcccc gctccgacca ccagcacctg ctcgccggga
     6661 gcaatggctg cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc
     6721 gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca
     6781 ctaaaaccgc catcctgatg cacgccaatg actgaaattt tttcacagca attggtacgc
     6841 ccgcttttac acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga
     6901 ccatttttaa gatcagcaac atttttaccc agcccaacaa cctccccaca tatttcatgg
     6961 cctaaaaccc gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta
     7021 ccgcaaatcc cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggaatc
     7081 tcgcgttgtt tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac
     7141 atgattcggc tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg
     7201 gaacggaaaa tctgttttgt gaaaacacgc gcataaaaat gttttaaatc gggttttaat
     7261 ggactcacaa ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc
     7321 aggtcattga tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc
     7381 tggcggaaat gtacaacatc agccgcacta cagtgcgtca tattctcagc cacttacgcg
     7441 aatgcggcgt cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg
     7501 atgacggttt cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg
     7561 cttttttcac gatgataaac cagcgccagt tacgcccagg ggaaactttt tctgaactgc
     7621 aactggctcg ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg
     7681 gccgttataa tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc
     7741 aatcctacgc cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc
     7801 actttctcaa cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctcgaac
     7861 gtcacaggct actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca
     7921 gagattttca ctccttactg ctttcagctg ccgacaatat cttttttgaa caatcgcttg
     7981 agatcatatc agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc
     8041 gcaatatcat tgccgtcgat gagcatatga ccattctcag cgctctgatt tgccgcagtg
     8101 accttgatgc cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc
     8161 gatccattaa tgagaacatg cgttatgctc attaagtacg gattaaaaac agcaaactat
     8221 tagcgggtgc acggttttat caaaagataa ataccggaaa attcccgctt acctatgctc
     8281 acaatcagtc gactaaaaat gtttttttgt gagcggtagt aaagtcctaa aactttaacc
     8341 tgccgttaat gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa ttataaatac
     8401 aggagtccgg cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca tttactccat
     8461 cttcgtcggc agatattccc gtgccaccag atggattagt tcaacgcagt acccgaatta
     8521 aacgcattca aaccaccgcc atgttgttat tattttttgc ggcggtaatc aattatctcg
     8581 accgcagttc gctgtcggta gcaaatttaa cgattcgtga agaattggga ttaagtgcca
     8641 ccgaaatcgg cgctttgctc tccgtgtttt cactcgctta cgggattgcg caacttcctt
     8701 gcggcccact attggatcgt aaaggcccgc gcctgatgtt gggactgggg atgttcttct
     8761 ggtcactgtt ccaggcaatg tctggcatgg tgcacagctt tacgcagttc gtgttggtgc
     8821 gtatcggtat gggaattggt gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg
     8881 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
     8941 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
     9001 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
     9061 atcgcaaccg cgagcacgta gaactgactg ccgttgaaca agcttatctc aatgcaggta
     9121 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcttg ttccgtaacc
     9181 gcacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
     9241 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
     9301 tgatggcggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
     9361 ctgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
     9421 ccgggatgtt ctgttctgcc gcctttacgc tggtcgtacc gcaagcgaca acatccatga
     9481 cagcggttct gctgattggt atggcactgt tctgtattca ctttgccgga acatcctgct
     9541 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
     9601 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
     9661 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
     9721 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acggaaagat taatctccct
     9781 atgcccgata acattttgct tatcgggcat ttttatggcg attaaaacgt ttcccagttc
     9841 tcaccgctat ctgccacggc cattttacgc ggcgcagctg gcatcacggt ttttaccaca
     9901 gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc cactgcttcg
     9961 gtcaggcgac tggcctgctc ttccagcgcg gcggcggcgg cggcagattc ttccaccagc
    10021 gcggcgttct gttgagttac ccggtccatc tcagcaaccg ctaagccaac ctgatcgata
    10081 ccacggctct gctcatcaga agcagaagca atttcgccca taatgtccgt cacgcgggtc
    10141 acagcactga caatctccgc cattgtttcc ccggcgcttt cgaccagcgt agagccaaca
    10201 tccactttcc ccaccgagtc ttcaatcagg cttttaattt cacgagccgc ctgggcgcta
    10261 cgctgggcca gattacgcac ttctcccgca accaccgcaa aaccacgtcc ttgctcaccc
    10321 gcacgcgctg cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa ggcaatgccg
    10381 tcaattacgc tgataatatc ggcgattttc tgcgaactgg tggagatatc acgcatagtc
    10441 tgcaccacgt tatctaccac tttgccgccg cgttgcgccg tttcagaagc acttaacgcc
    10501 aggtggctgg cctggcgcgc attctcggcg ttctgtttca ccgttgcggt cagttgctcc
    10561 atgctggctg ccgtctcttc cagcgaagca gcctgttgct cggtgcgcga agagagatcg
    10621 ttattgccgg tagcgatttc gctggcaccg ctatagatgg cattggcccc gttgcgcaca
    10681 tcaccgacgg tacgcatcag ctctccctgc atatggcgca aactctctgc cagttgcccc
    10741 atctcattag agccatccac ctcaatcggt ttcaccagat cgccgcctgc aatatgacga
    10801 atgctgtcaa tcaggcgatt cattggcgct accagcgagg ctttaatacc gaaccagacg
    10861 gcgaagatga ccgccagtac gacgatcatc acgcccacca gaatccacat cgcctggctg
    10921 taggaggcat tgttatcgct gacggcgata tcatggagcc gatcgttttg ttccatgtaa
    10981 gccacatact gcttctcgaa accgtcctga tatccctgag tcggctgatc aaagaactcg
    11041 ttgattttgc ctgcacctaa cagttggatc agctccgcca gcgcattgtg ataaatatcg
    11101 taattacgtt tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct
    11161 tcgtaatccg cccagttttt ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc
    11221 tcagcaacgg ttgaaccgct accaatatta ttctgatcca tcatgtagcg gatacccgcg
    11281 cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc tgccattcag cgtggattgc
    11341 tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt cattctttaa ggcattaaag
    11401 aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg
    11461 gtcacaattt tgatacgttt taacatggtt tctctttcct gtggaccgat gcaagatttt
    11521 cggcctggaa aggaaaaact ttatgaatat cccgcggaga ttacgccgag taattttatt
    11581 cacactctga atttaaaaag c
//
LOCUS       DAPQZD010000151        11571 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPQZD010000151 DAPQZD010000000
VERSION     DAPQZD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.153"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..597
                     /locus_tag="RO454_003919"
     CDS             1..597
                     /locus_tag="RO454_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDY0935675.1"
                     /translation="MNYPAEYEIGDIVFTCIGAALFGQISAASNCWNNHVGIIIGHNG
                     EDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKL
                     YHTGFKYESSRQFCSKFVFDTYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGS
                     IPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(599..1120)
                     /gene="ruvC"
                     /locus_tag="RO454_003920"
     CDS             complement(599..1120)
                     /gene="ruvC"
                     /locus_tag="RO454_003920"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDY0935676.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1155..1895)
                     /locus_tag="RO454_003921"
     CDS             complement(1155..1895)
                     /locus_tag="RO454_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDY0935677.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1924..2376)
                     /gene="nudB"
                     /locus_tag="RO454_003922"
     CDS             complement(1924..2376)
                     /gene="nudB"
                     /locus_tag="RO454_003922"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDY0935678.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2369..4141)
                     /gene="aspS"
                     /locus_tag="RO454_003923"
     CDS             complement(2369..4141)
                     /gene="aspS"
                     /locus_tag="RO454_003923"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDY0935679.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4451..5017
                     /locus_tag="RO454_003924"
     CDS             4451..5017
                     /locus_tag="RO454_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDY0935680.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5014..5832
                     /locus_tag="RO454_003925"
     CDS             5014..5832
                     /locus_tag="RO454_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDY0935681.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRYAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5885..6280
                     /locus_tag="RO454_003926"
     CDS             5885..6280
                     /locus_tag="RO454_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDY0935682.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6321..7064
                     /gene="cmoA"
                     /locus_tag="RO454_003927"
     CDS             6321..7064
                     /gene="cmoA"
                     /locus_tag="RO454_003927"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDY0935683.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7061..8032
                     /gene="cmoB"
                     /locus_tag="RO454_003928"
     CDS             7061..8032
                     /gene="cmoB"
                     /locus_tag="RO454_003928"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDY0935684.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8068..10497)
                     /gene="torZ"
                     /locus_tag="RO454_003929"
     CDS             complement(8068..10497)
                     /gene="torZ"
                     /locus_tag="RO454_003929"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDY0935685.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAIQA"
     gene            complement(10522..>11571)
                     /locus_tag="RO454_003930"
     CDS             complement(10522..>11571)
                     /locus_tag="RO454_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDY0935686.1"
                     /translation="VGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQ
                     KGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWK
                     ELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGETCIDCHKGIAHFPPEIKM
                     DDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTDLTVVDVSGKQPIVRLQG
                     YQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDS
                     ALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDIL
                     TRYFQYNAKDITEKQ"
BASE COUNT         2622 a         3065 c         2850 g         3034 t
ORIGIN      
        1 attaattatc ctgcggaata tgaaattggt gatatcgtct ttacatgtat aggtgctgcc
       61 ttatttggtc aaatatcagc tgcatcaaat tgctggaata atcacgtcgg gatcattatc
      121 ggtcataacg gtgaagactt tctggttgca gaaagccgtg ttcccctctc aaccatcact
      181 acgctatccc gttttattaa acgctctgct aatcaacgct atgctataaa gcgattagac
      241 gccggactaa cagaacaaca aaaacaacga attgttgaac aggttccttc ccggctacgc
      301 aaactttacc acaccggttt taaatacgaa tcttcgcgac agttctgttc aaaatttgtt
      361 tttgatactt ataaagaggc gctatgtatt ccggtgggtg aaatagagac gtttggagaa
      421 ttattaaata gcaatccgaa tgcaaaactc actttctgga aattctggtt cttaggttct
      481 attccgtggg agcgtaaaac cgtcacgcca gccagtttgt ggcatcatcc gggtttggtg
      541 ttgattcacg cggtgggagt tgaaacgcct cagcctgaac tgaccgaggc ggtataactt
      601 aacgcagtcg ccctctcgcc aggttcagtc gcgattcact catttgcatc gcattctgac
      661 taacgtggca gtgggtgatg gcaatcgcca gcgcatcggc ggcatccgcc tgtggattag
      721 cgggcagttt cagcaaggtg cggaccatat gctgcacctg gcttttttcg gcactaccaa
      781 tacctaccac tgtttgcttt acctgacgtg ccgcatattc aaataccggc aattcctgat
      841 tcaccgccgc cacaatcgcc acgccgcgcg cctgccccag tttcagggct gagtcagcgt
      901 tcttcgccat aaagacttgt tcaatggcga aataatcagg ctggaattgg gtgatgattt
      961 ccgtcacgcc cgcatagatg agcttcagac gagacggtaa atcatccact ttggtgcgta
     1021 tgcatccgct acccaggtag gacagttgcc tgcctacctg gcggatgacg ccatagccgg
     1081 tcacgcgcga acccgggtca ataccgagaa taatagccat cacgcgtctc cgttttgctg
     1141 tttagcaggc ctcatcagag agtcgctgca acctcatcag agatttcacc gttatggtaa
     1201 acttcctgca cgtcgtcgca atcttccagc atatcgatca gacgcatcag tttcggtgcg
     1261 gtttctgcat ccatatcagc tttggtggac gggatcatgg aaacttccgc gctgtctgct
     1321 ttcagacctg ccgcttccag agcgtcgcgt actttgccca tttcttccca tgcagtgtag
     1381 acatcaatcg cgccgtcatc ataggtcaca acgtcttcag caccggcttc cagggctgct
     1441 tccatgatgg tgtcttcatc gcctttctcg aaggagatca cgcctttttt gctgaacaga
     1501 taagctacgg aaccatcagt accgaggtta ccgccacatt tgctgaatgc atgacgcact
     1561 tcagcaacgg tacggttgcg gttgtcagac agacattcaa tcatgattgc cgtgccgcca
     1621 ggaccgtaac cttcgtagat gatggtttcc atgtttgcat catcatcacc gcccacacct
     1681 cgtgcaattg cgcggttcag agtgtcacgg gtcatgttgt tagacagtgc tttatcaatt
     1741 gctgcacgca gacgcggatt agcgtccgga tcaccaccgc ccagcttagc cgcggttacc
     1801 agctcacgaa tgattttagt gaagatttta ccgcgcttag catcctgcgc agctttacga
     1861 tgtctggtgt tggcccattt actatgacct gccataaaaa tatctccaga tagccctgcc
     1921 tgttcaggca gcgttaatta caaactgttc aatcgcctgc cggttgctcc aggacttagt
     1981 gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc agatgttcag tgaaaacgat
     2041 ctggcgctcg tgcgggagcg caagacagaa ccatgattcc gtattacgcg tcacgcccgg
     2101 cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct accgtgcgct gacagtcaat
     2161 taaggtcagt tgttcagcga caacatcaat ggtgacctct tcctttactt cgcgcatggc
     2221 agcttgcggc gcggtttcac cctcttccac gctgccggtt accgactgcc agaaatcggg
     2281 atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct tgtgcgtaga tgaccactaa
     2341 gatcgaaacg ggacgcttat aagccatatc agttattctc agccttcttc acaacctgaa
     2401 tgctcagctc agccagtgca gtcgggttag caaagctcgg tgcttcagtc atcagacacg
     2461 ccgccgccgt ggttttcggg aaggcgataa cgtcacggat attgtcggtg ccggtcagca
     2521 gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg cggcggagta ccgtatttca
     2581 gggcgtcgag caggaagccg aatttctcgc gctgttcctc ttcgttgata cccagaatac
     2641 caaacaccgt ctgctgcata tcaccattat ggatacgcac agaaccaccg cccacttcgt
     2701 aaccattgat gaccatatcg taagcgttag ccaccgcatt ttccggtgca gctttcagtt
     2761 ctgctgccgt catgtctttc ggtgaggtga acggatggtg cattgctgtc aggccgcctt
     2821 caccgtcgtc ttcaaacatc gggaagtcga taacccacag cggtgcccat ttgctttcgt
     2881 cggtcagacc aaggtcttta cccactttca ggcgcagtga gcccatcgcg tcggcaacaa
     2941 ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc ttgcgcgcca gtacgctcca
     3001 ggatggcttc gatgatttct gcattaagga acttcgctac cgggctgttg ataccttcca
     3061 gacctttcgc gcgttcgtta actttgatgt aagccagacc tttcgcgccg tagattttaa
     3121 cgaagttacc gtattcgtcg atctgcttac gggtcaacga tgcgccgccc ggaacacgca
     3181 gagcggcaac acggcctttc ggatcgttcg ccggacctgc aaatactgca aactcaacag
     3241 atttcagcag atcggcaacg tcggtcagtt ccatcgggtt acgtagatct ggtttatcag
     3301 aaccataacg gcgttctgct tctgcaaagg tcattaccgg gaaatcgccc agatccacgc
     3361 ccttcacttc cagccacaga tgacgcacca gcgcttccat cacttcacgc acttgcggcg
     3421 cggtcatgaa agaagtttcc acatcgatct gagtaaattc aggctgacgg tcagcacgca
     3481 ggtcttcgtc acggaagcat ttaacgatct gatagtagcg gtcaaagccg gacatcatca
     3541 gcagctgttt gaacaactgt ggggattgcg gcagtgcgta gaatttacct ttgtgcacac
     3601 gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc tttggtcagc atcggagttt
     3661 cgatgtcgag gaagccgtgg tcatccataa aacggcgcac caggctggtg attttagcgc
     3721 gggttttcag gcgctgagcc atttccgggc gacgcaggtc gaggtagcgg tatttcagac
     3781 gcgcttcttc ggtgttgacg tggttagagt caagcggcag aacatctgca cggttgatga
     3841 tagtcagcga ggacgccagt acttcgattt cgccagtcgc catatcgcgg ttaatatttt
     3901 tttcgtcacg cgcacgtacg gtgcccgtga cctgaatgca gaactcatta cgcagttcag
     3961 aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa tacctgcacg ataccttcgc
     4021 ggtcgcgcat atcgatgaag atcaggctac caagatcacg acgacggttg acccaaccac
     4081 acagagtcac ctgctgcccc acgtgggaca aacggagctg tccacaatat tctgtacgca
     4141 tgagatatcc cttaacttag ctgccggcgg atgccccctg ctgcgcaggt gaccaagtcg
     4201 cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg ctattatact ggaaattctg
     4261 ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt accactttat atcgggcctg
     4321 aaatcagact ctacgccagt ttgctataaa ggtgttgccc gaactcataa aaattaacaa
     4381 aatttgtcgt tccgccatcg gctcatcgta ttaaggtgag aggcacgatt ttgttttatc
     4441 aggagtcatc atgcttgaac ttaatgctaa aaccaccgcg ctggtggtga ttgatttaca
     4501 agaaggcatc ttgccttttg ccggtggtcc acatactgcc gatgaggtgg ttaatcgcgc
     4561 cgggaagctg gcggcgaaat ttcgcgcgag cggtcagccc gtgtttctgg tgcgtgttgg
     4621 ctggtctgcc gattatgctg aagcattaaa acagccggtt gatgcccctt cccccgctaa
     4681 agtgttgccc gaaaattggt ggcaacatcc tgtagcatta ggtgcaaccg acagcgatat
     4741 cgaaatcatc aaacgtcaat ggggtgcgtt ttacggtacg gatctggaat tgcagttgcg
     4801 ccgccggggt atcgatacaa tagtgttatg tgggatctcg accaatatcg gtgttgaatc
     4861 caccgcccgc aatgcctggg aactcggttt taatctggtg attgccgaag atgcctgtag
     4921 cgccgctagc gccgagcagc acaataacag cattaatcat atctacccgc gcatcgcccg
     4981 tgtgcgtagc gttgaagaga tcctccacgc gttatgattt acatcggttt gccacaatgg
     5041 tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt
     5101 aactgtgtgg agggtaacac cacgctttat gccctgccga aacccgaggt tgtcctgcgc
     5161 tggcgtgagc agaccacaga tgacttccgc ttctgtttta agtttccggc gactatttcg
     5221 catcaggcag cattacggca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca
     5281 ccgttggctc cgcgcattgg acaatactgg ctgcaactgc ctgccacatt cggcccacgg
     5341 gagctgcctg cgctttggca ttttctcgat tctcttcccg gcgaatttaa ttatggtgtg
     5401 gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt
     5461 ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcagcacgt
     5521 ccacacagtg aagctattcg ctacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct
     5581 gtactgacgg cgaaaaatcc actgatccgt tttatcggta gtgatgatat gacgcaaaac
     5641 cgggaattat ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct
     5701 tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg
     5761 gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct
     5821 tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt
     5881 ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt
     5941 tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggcgacggcg gttttagcga
     6001 actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt
     6061 gttgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat
     6121 cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgttccgctg
     6181 gcgacgttct ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct
     6241 ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct
     6301 ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca
     6361 gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc
     6421 gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg
     6481 ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acactctcgg
     6541 tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgataac tccccggcga
     6601 tgattgaacg ctgccgtcgt catattgacg cctataaagc tcctacgcca gtagacgtta
     6661 ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta
     6721 ccctgcaatt cctggaacct tccgagcgcc aggcgttact ggataaaatt tatcaaggac
     6781 tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg
     6841 gtgaactgct gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg
     6901 agatcagcca gaaacgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc
     6961 ataaagcacg cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta
     7021 actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt
     7081 ttattctctg attgccaaaa atcatctttc acactggctc gaaacgctgc ccacgcagat
     7141 tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acgcggtgga
     7201 atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag
     7261 cgaagagcca ctgagcgccg ggcaaattaa gcgcattgaa acgctgatgc gcaacctgat
     7321 gccgtggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc
     7381 cgactggaaa tgggatcgcg ttctgcccca tctttctgat ttaaccgggc gcaccattct
     7441 tgatgtcggc tgcggcagcg gttatcacat gtggcgcatg attggcgctg gcgcgcacct
     7501 cgcggtgggg atcgacccta cacaactgtt tctgtgccag tttgaagcgg tgcgtaaact
     7561 actgggtaac gatcagcgcg cacatttgtt accgttaggt attgaacaac ttccggcact
     7621 gaaagccttt gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga
     7681 gcatctctgg cagttaaaag accaactggt gaatgaaggc gaactggtgc tggaaacgct
     7741 ggttattgat ggcgatgaga acacggtgct ggtgccgggc gatcgttacg cacaaatgcg
     7801 taatgtctat ttcattcctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt
     7861 tgttgatatt cgcattgcgg atgtgagcgt taccaccaca gaagagcagc gacgcaccga
     7921 atggatggtc accgagtctc tggccgattt tctcgacccg catgatccgg gtaaaacggt
     7981 ggaaggttat cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaaatttag
     8041 cgcccgtttc cgggcgcttt ttacgtctta tgcctgaata gctggctgat caaacgccgt
     8101 taacttcagc gcattgcccg tatatttttc gatatacacc agcgcagagt taccggcaca
     8161 ggcattcgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac taccgttttt
     8221 acacaagcca ttttccagat ctggccatgc accttcatgc acgcatacca cgcctttttt
     8281 gatcccgtca gtcaccaccg cgcctgtcag aatctgacca cgtttgttcc agacacgcac
     8341 cagatcgcca ttcgcaatac caaagcgagc agcatcttcg gtgtgaatag tgattggttc
     8401 acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac tgtgtaaacg
     8461 gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac ccttccactc
     8521 atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt tttccagcgt
     8581 tctggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca ccggatcggc
     8641 gcggaaatca ccataacgaa tgtactgttc gttcttctcg ctgcggcgca tttcgatcag
     8701 tttattttgc tgccagaagg cattaaacat cggcatcgtc acgcgttgcg cacgggcacc
     8761 tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac cttcggtata
     8821 gatctctttt ccgccaggtt tgagtaattc agcaagatcg gcaaacacgt caaaatcgtt
     8881 gcgcgcttca aattgcggag cgacagcctg cttcatcggc acaatatgct ggttgctgta
     8941 atcaccggtc atcgtcaggt cattgcgctc aaacgatgtg gtgatcggta atacgatatc
     9001 tgcgtgtttg gctgctgcgg tccagtagca ttcagaaacg acgatcatct ccggtttctg
     9061 ccatgcttta atcagacgat tagtatcctg gtgatgggta aagttaccgc cacccgccca
     9121 ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat atttgccgcc
     9181 aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgtcattc cgccatcatc
     9241 agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tctggtagca cgccgccaac
     9301 acgcgtcggg ttgccaccgt tggagtaatg ataagagaaa ccaaacccgc cgcccggtgt
     9361 accaatttgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct gttcaccgta
     9421 ttgctggcgc tgaattcccc agcctgccat cagcattgtg cgatttgccg ccatcagttc
     9481 tgcaagtttc acaatctgcg cttccggcac gccagtaatt tccgccgccc aggccgcact
     9541 cttcggcgta ttatcgttct tgcctgtcag atactcttca aactgcggat acccggtagt
     9601 gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat gcgcaatccc
     9661 taacatcagt gccacgtcgg tgcccatatt cggtgcgatc caggtggcat tatcgccaaa
     9721 gaattcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc cagatttttt
     9781 cagctgatgg aagtattcca gcccttgttc atcggtactg ctccaggcaa tttttaaggt
     9841 attcagcggg ttcatgcccc acaacaccac cacctgactg ttttccagaa tcagcggcca
     9901 ggaagtctgc tgttcataga cctccacaga accgaccaca tgcggcatga tcacctgtgc
     9961 cgctccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt tcatataacg
    10021 ttgcagtaag gtttgcgctt tatgcaacac gccagaagaa cgccagccgt aagatccggc
    10081 aaaaatggcc gatggtccgt tagctttacg aatatgatcg tgttgctcat gaatcagttt
    10141 taatgcttgt tcccagctca cctgtacata ggtatcttcg ccacgacctt tcgccggttg
    10201 cagtggatta tcgagatagc tttttctcac catcggatgc tgaatacgcg ccgtggtgtg
    10261 tacctgatcc gccgccgtag actgtaagga gttcggtatg gtcttcgcca gcgcgcctgt
    10321 cgaagaaaca atcttgccgt ccttcacttc tacgttcatc gctccccaac gtcccgcggt
    10381 gagaattttg ccgcccttct cttctgccca tgcaggtaac ggtgcagccg atgtcaccac
    10441 cagcgcccca gcggcaatac cgctatgttt aataaattcg cgtcttgtta atgtcataac
    10501 ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt tgtactggaa
    10561 gtaccgcgtc aaaatgtcca gttcgttttc gctcatgctg gttcgcgcac ccattccttt
    10621 ggcaatggac ggccatgcat tgacggtgta atggtcggcg gcaatagggg catggcagcc
    10681 agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agtggtttac ggtccgctaa
    10741 tgcggaatcg gttagcgcac cctgtaaaga cgcctgacgc cattgattgc cgtattcgtc
    10801 agcctgccat tccccgttta ccgttagcgc cttgatacct tcttcactta atgtggcgag
    10861 cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca tttgataacc
    10921 ctgcaaccga acaattggct gtttaccact gacatcaacg acggtgagat cggtaccagg
    10981 agtcacggta gccaggtcgc ctatgcgaga agttttgaaa ggataaatat gtgcgccatt
    11041 agtaactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat ccatttttat
    11101 ttctggcggg aaatgggcaa tgcctttatg gcaatcgata caagtttcgc cgtccttttg
    11161 cgctttatta tgcattttct gcgcagattc actttgcgag gaaatatcca tggcatcaaa
    11221 agaatggcaa ctacggcagg ttgcagagtc agttgctttt aattctttcc atactgtttc
    11281 ggccatttcc tggcgatgag cttcgaactt atcgtcactg tctattttgc cgctaacaaa
    11341 ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca tccctgactt
    11401 tggaatatgg caatcggcac attccgcgcg tatacctttt tggttcgaaa agtggacagt
    11461 tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa atgcagtatc
    11521 cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa c
//
LOCUS       DAPQZD010000152        11435 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPQZD010000152 DAPQZD010000000
VERSION     DAPQZD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.154"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..983)
                     /gene="pyrD"
                     /locus_tag="RO454_003931"
     CDS             complement(<1..983)
                     /gene="pyrD"
                     /locus_tag="RO454_003931"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDY0935687.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPL"
     gene            1220..1795
                     /gene="ssuE"
                     /locus_tag="RO454_003932"
     CDS             1220..1795
                     /gene="ssuE"
                     /locus_tag="RO454_003932"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDY0935688.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1788..2747
                     /gene="ssuA"
                     /locus_tag="RO454_003933"
     CDS             1788..2747
                     /gene="ssuA"
                     /locus_tag="RO454_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDY0935689.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2744..3889
                     /gene="ssuD"
                     /locus_tag="RO454_003934"
     CDS             2744..3889
                     /gene="ssuD"
                     /locus_tag="RO454_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDY0935690.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3901..4692
                     /gene="ssuC"
                     /locus_tag="RO454_003935"
     CDS             3901..4692
                     /gene="ssuC"
                     /locus_tag="RO454_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDY0935691.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4689..5456
                     /gene="ssuB"
                     /locus_tag="RO454_003936"
     CDS             4689..5456
                     /gene="ssuB"
                     /locus_tag="RO454_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDY0935692.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(5499..8111)
                     /gene="pepN"
                     /locus_tag="RO454_003937"
     CDS             complement(5499..8111)
                     /gene="pepN"
                     /locus_tag="RO454_003937"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDY0935693.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8377..9579
                     /gene="pncB"
                     /locus_tag="RO454_003938"
     CDS             8377..9579
                     /gene="pncB"
                     /locus_tag="RO454_003938"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDY0935694.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9748..11148
                     /gene="asnS"
                     /locus_tag="RO454_003939"
     CDS             9748..11148
                     /gene="asnS"
                     /locus_tag="RO454_003939"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDY0935695.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2632 a         2941 c         3004 g         2858 t
ORIGIN      
        1 agcggcggac ctttaaaaat aaaaccagaa taaatttgca ccagtgaggc acccgcagca
       61 atcttttcac gcgcagcgat aaccgagtca atgccgccaa caccaataat cggcaagcgt
      121 ccgtttaatt cctgtgacaa gcggcgaata atttcggtgc tttttaactg aagcggacga
      181 ccacttaagc caccggtttg atcacaattt ttcattccct gaaccagaga acgatcgagt
      241 gtggtattgg ttgcaataac gccatcaata ttatggcgaa ctaaactatc ggcaacctgg
      301 atcaattctt cttcagaaag atccggcgcg atcttcactg cgatcggcac atatttatgg
      361 tgcatcactt gcaaatcatt ttgtttattt ttaatcgcag ttaagagatc atccagcgct
      421 tcaccatatt gcagtgtgcg taatcctggg gtatttggcg atgaaatatt gatggcgata
      481 tatcccgcat aggcatagat tttttccata caaatcagat agtcatcttt accctgctcc
      541 accggcgtat ctttattttt gccgatgtta atacccagaa cgccgtcata atgggctttt
      601 tttacgttct ctacgaggtt atcaacgcca agattattaa agcccatacg gttgatcaaa
      661 ccttcggcat ctaccagacg aaagagacgc ggcttgtcat tacctggctg gggacgtggc
      721 gtgacggtac cgatctcgat ggatccaaat cccatcgcgc ctaacgcgtc aatgcactcc
      781 ccgtctttat caagaccggc tgccagacca agcggatttt taaacgtcag gcccatgcag
      841 ttaacaggtt tcgcaggcac tttctgccgc accagtgctt caaacggcgt tcctgtaata
      901 cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg gatcgagctg gaaaagggct
      961 ttacgaacga aggggtagta catgaactct cctggattcc cggtgtgcaa accgggggcg
     1021 tattatgtgc gagttcggac caaaagggaa ttgacctgcg gcaataaata gcaaacgttt
     1081 tcttattgcg ctttttttat gctttttttg gcttttcttc ctcagataaa tcatttcgac
     1141 tcgcggacgc cctctgtcag actgaccgaa ttgttatcaa tgttaacaaa acaagaacaa
     1201 ttggttataa ggagagagta tgcgtgtcat caccctggcg ggtagtcctc gatttccttc
     1261 tcgctccagc tccttgctgg aatatgcgcg ggaaaaacta aatggcctgg atgtagaggt
     1321 ttatcactgg aatttgcaaa acttcgtccc ggaagatctg ctttatgctc gtttcgatag
     1381 tccggcactc aagaccttca ccgaacagct gcaacaggcc gatgggctga ttgtcgccac
     1441 gcctgtgtat aaagccgcct attccggtgc actgaaaacc ctgctcgacc tgctgccaga
     1501 acgtgcgttg caaggcaaag tggtgctgcc gctggcgaca ggcggcaccg tggcccatct
     1561 gctggcggtc gattatgccc ttaagccagt tttaagtacg ctgaaagccc aggagatcct
     1621 gcacggcgtg tttgccgatg actcgcaagt aattgattac caacacaaac cccagttcac
     1681 gccaaatctg caaactcgtc ttgataccgc gctagaaact ttctggcagg cgttgcaccg
     1741 ccgcgatgtt caggttcctg acctactgtc tttgcgaggt aatgcccatg cgtaagatca
     1801 ttaaactggt gctggcgggt ttacttagcg tctctacgat tgcggttgct gcagaatcct
     1861 cgcctgaagc gttacgtata ggctatcaaa aaggcagtat tggtatggtg ctggcaaaaa
     1921 gccaccagtt actggaaaaa cgctatccgc aaacaaaaat ctcctgggtc gagttccccg
     1981 ctggcccaca gatgctggag gcgttaaacg ttggcagtat cgatctcggc agtaccgggg
     2041 atattccgcc aatctttgcc caggctgccg gggctgattt ggtatacgtg ggcgtcgagc
     2101 caccgaagcc caaagccgaa gtgattctgg tggcagaaaa cagcccgatc aaaaccgtag
     2161 ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc cagttcgcac aatcttttac
     2221 tgcgagccct tcgccaggcc gggcttaaat tcacggatat tcagcccact tacctgacgc
     2281 ctgccgatgc ccgcgccgcg ttccagcaag gtaacgttga cgcctgggct atctgggatc
     2341 cctactactc cgctgcatta ttacagggcg gcgtgcgggt gctgaaagac ggcaccgatc
     2401 tcaatcaaac tggatcgttt tatctggcag ctcgtccgta tgcagaaaaa aacggcgctt
     2461 ttattcaggg tgtgctgaca acctttagtg aggccgatgc gttaacccgc agccagcgcg
     2521 aacaaagcat cgctttactg gcaaaaacga tgggcttacc ggcaccggtt attgcctcgt
     2581 atctggatca tcgtcctccc accaccatca aaccgttgag cgctgaagtt gccgccttac
     2641 agcagcaaac ggcagatctg ttttatgaaa accgtctggt gccgaaaaaa gtcgatattc
     2701 gccagcgcat ctggcagccc actcaactgg aaggaaaaca attatgagtc tgaatatgtt
     2761 ctggttttta ccgacccacg gtgacgggca ttatctggga acggaagaag gttcacgtcc
     2821 ggttgatcac ggttatctgc aacaaattgc gcaagcggcg gatcgtctgg gctataccgg
     2881 tgtgctgatc ccgacggggc gatcgtgtga agatgcgtgg ctggtggcgg cgtcgatgat
     2941 cccggtgaca caacggctga agtttcttgt cgccctgcgc cccagcgtaa cctcacctac
     3001 cgttgccgcc cgccaggccg ccacgcttga ccgtctctct aacggacgtg cgttgtttaa
     3061 cctggtcaca ggcagcgatc cccaagaact ggcaggcgac ggcgtgttcc ttgatcatag
     3121 cgagcgttac gaagcctcgg cagaatttac ccaggtctgg cggcggttgt tgcttggcga
     3181 gactgtggat ttcaacggca aacatattca tgtacgcggg gccaaactgc tcttcccgcc
     3241 gattcaacag ccttatcctc cgctttactt tggcggatcg tcagatgtcg cccaggagct
     3301 ggcggcagaa caagttgatc tctaccttac ctggggcgaa ccaccgaaac tggtaaaaga
     3361 gaaaatcgaa cacgtgcggg cgaaagctgc cgcgcatgga cgcaaaattc gtttcggtat
     3421 tcgtctgcat gtgattgttc gtgaaaccaa cgacgaagcg tggcaggccg ccgagcggtt
     3481 aatctcgcat cttgatgatg aaactatcgc caaagcacag gcctcattcg cccgaacgga
     3541 ttccgtagga caacagcgaa tggcggcgct acataacggc aagcgcgaca atctggagat
     3601 cagccccaat ttatgggcgg gtgttggctt agtgcgcggc ggtgccggga cagcgctggt
     3661 gggcgatggt cctacggtcg ccgcgcgaat caacgaatac gctgcacttg gcatcgacag
     3721 ttttgtgctt tcgggctacc cgcatctgga agaagcgtat cgggtcggcg agttactgtt
     3781 cccacatctg gatgtcgcca tcccggaaat tccccagcca caaccgctga atccacaagg
     3841 cgaagcggtg gcgaatgatt ttatcccccg taacgtcgcg caaagctaag gaacacgatg
     3901 atggcaacgc cagtgaagaa gtggttattg cgcgttgccc cctggttttt accggtgggc
     3961 atcgtggcgg tgtggcaact ggcctcctcg gttggctggc tatcgacgcg tattttgccc
     4021 tcaccggaag gagtggtgat ggcgttctgg acgctctctg ccagcggcga actgtggcag
     4081 catctggcga tcagctcctg gcgtgcactg attggttttt caattggcgg atcgctggga
     4141 ttaattctgg ggctgattag cgggctgtca cgctggggtg agcgactgct ggatacctca
     4201 attcagatgt tgcgcaacgt gccgcatctg gcgctgatcc cgctggtgat tttgtggttt
     4261 ggcattgatg aatccgcaaa aatctttctg gtggcgctcg gtacgctatt ccccatttat
     4321 atcaacacct ggcatggcat ccgtaatatc gaccgtgagc tggtggagat ggcacgtagc
     4381 tatggattat ccggcatgcc gctgtttatc catgtgatcc tgcctggtgc cctgccctca
     4441 attatggtcg gtgtgcgttt tgctttgggc ctgatgtggc tgacgctgat cgttgccgaa
     4501 actatttctg ccaattccgg cattggttat ctggcgatga atgcgcggga gtttctgcaa
     4561 acggacgtgg tggtagtcgc cattattctt tacgccctgc tcggcaaact tgctgacgtc
     4621 agcgcgcagc tgttagagcg tctctggttg cgctggaacc cggcttatca tttgaaggag
     4681 gccacggtat gaatactgct cgtctgaacc agggcacacc attgttgctc aatgcagtaa
     4741 gcaaacatta cgcggaaaat atcgtcctga accaactgga tttacatatt ccagcaggtc
     4801 agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag taccctgctg cgcctgctgg
     4861 caggtctgga aacgccaacc gcaggcgacg tgttagcggg caccacaccg ctggctgaga
     4921 ttcaggatga tacgcgaatg atgtttcagg acgcgcgtct gctgccgtgg aaatcggtga
     4981 ttgataacgt tgggttaggc cttaaaggcc agtggcgcga tgccgcgcgt cgggctctgg
     5041 ctgcggtagg actggagaat cgcgcggggg aatggcctgc cgcactttct ggcgggcaga
     5101 aacaacgtgt ggcgctggca agggcgctga ttcatcgacc aggtttattg ttgcttgatg
     5161 aaccactcgg tgcgctggac gctttaacgc gactcgagat gcaggatttg attgtatcac
     5221 tctggcagga gcacggcttt accgtcttgc tggtgacgca tgatgtgagc gaagcggtgg
     5281 cgatggctga ccgggtgttg ttaattgaag agggaaaaat tggtctggat ttgacggtgg
     5341 atatcccccg tccgcgccgg ttagggtcgg tacgattagc agagctggaa gcggaagtat
     5401 tgcagcgggt gatgcagcgt ggtgaatttg aaactcgcct gagaaaacag gggtaaattc
     5461 tccgaatggc ggcgctattg ccgccattcg gttatttatc aagccagtgc tttggtgatc
     5521 ttctcgtaca gatcgccaga gagattttcc agccctttca actgttccag cgccgcgcgc
     5581 attttctcct gacgtttggc atcgtaacgt ttcaggcgaa tcaacggttc aatcaggcgc
     5641 gaagccactt gtggatttcg gctgttcagg tcggtaagca tttccaccag gaactgataa
     5701 ccgctgccat cttcggcatg gaacgctgcc gggttgctgc ccgcaaatgc gccaatcaat
     5761 gaacgaatgc ggttcgggtt gctcatggta aatgagcgat gctgcaacag accacgtacc
     5821 gtctccagca cattcgccgc cgggctggtg gcttgcagga taaaccattt atccatcacc
     5881 agaccatcct gatgccactt gtcgtcgtac tcctgcatca gcgcgtcacg gcatggcagt
     5941 tgtgcagcaa ccgccgcaga aagcgccgcc agcgcatcgg tcatattgtt cgcttcgtgg
     6001 aactgtttgc tcaccagcac atcagccaga tgcgtttcac caaaagcgag gaagcgcagg
     6061 caggcattac gcagagtgcg ttttgcaata tcttcatgct caacacggta ctcgctctgg
     6121 taattcgcgt tgtaaatagc cagcagctca tctgccagtt cagtcgccag agtacgagtg
     6181 agtgcttcgc gtacttcggc aatagcaatc ggatcgatga tatcgaacag ttctgccatt
     6241 tcattgacgg aaggcagcgt caggatttct gccgccagcg ccggatcaat cttctcatcg
     6301 agcagcaccg cgcggaaagc gtcagccaca tgcaccggca gagacaacgg ctgcccttgc
     6361 tgatgacggg cgacgttcag cttgatgtag gttgccagca aactttgcgc cgcgtcccag
     6421 cgagagaaat cattacgcgc atgacgcatc aggaaggtca gttgctgatc gctccactta
     6481 tattccagtt tcactggcgc agagaattcg cacagcagcg caggcaccgg ctggaagtag
     6541 acattatcaa aaacaaaggt ctgttccgcc tgggtgacgt tcagcacgga attcaccgga
     6601 tgaccgcctt tctgcaacgg gatcactttg ccttcgttgt catacagttc gatggcaaac
     6661 ggaatatgca gcggttgttt ttctgcctga tccggcgtag ctggcgtgcg ctggctgatg
     6721 gtcagggtgt actgctcagt ttccggattg tagtcgtctt tgacggtcac aaccggcgtg
     6781 ccggactggc tgtaccaacg gcggaaatgg gagagatcga cattcgacgc atcttccatc
     6841 gcctgcacaa aatcgtcaca ggtcgctgcg ctaccatcat gacgctcgaa atagagctgc
     6901 atccctttct ggaaattttc ttcgccaagc agagtgtgga tcatacgaat cacttccgcg
     6961 cccttctcgt aaacggtcag ggtgtagaag ttgttcattt caatgaccat atccgggcga
     7021 attgggtgcg ccatcgggct ggcgtcttct gcaaactgca atccgcgcat ggtgcgcaca
     7081 ttattgatac ggtttaccgc gcgggaacca aggtcagagc tgaattcctg atcgcggaag
     7141 acggttaaac cttctttcag actgagctgg aaccagtcgc ggcaggtgac acggttgccg
     7201 gtccagttat ggaaatattc atggccgata acgcgttcaa tatcgaggta atctttgtcg
     7261 gtggcggtgt cggtgcgggc cagcacatat ttggagttaa agatattcag ccccttattc
     7321 tccattgcgc ccatattgaa gaaatccacc gcgacgatca tatagatgtc gaggtcatac
     7381 tccaggccga agcgttcttc atcccatttc atggagtttt tcagcgaggt cattgcccac
     7441 ggcgcacgat caaggttgcc acgatcgaca tacaactcca gcgccacttc acgaccagag
     7501 cgggtagtga aggtatcgcg cagtacatca aaatcaccag ccaccagcgc aaacaggtaa
     7561 cacggtttcg ggaatggatc ctgccactgt acccaatgac gtccattttc cagttcacct
     7621 tgcgcaacgc ggttaccgtt ggaaagcagg aagggatatt tggttttatc ggcaataatt
     7681 ttggtggtaa aacgcgccag cacgtccggg cggtcgagat aatacgtaat atggcggaaa
     7741 ccttcggctt cacactgggt gcaaagcgca tcgcctgact gataaagccc ttccagcgca
     7801 gtattcgccg ccgggctaat ttcattaacg atcttaagcg taaaacgctc cggcaaatta
     7861 ctgatgacca gtgcgccctc ttcttctttc caggcggtcc acggctcatc attaatatga
     7921 acagaaacca gtttgaggtc ttcgccgttg agacgaagcg gaacatctga tgcaccatga
     7981 cggacagcct ggctgaccgc ggtaacgacc gtcttttgcg cgtccaggtc aaaggtcaag
     8041 tcaatatcag taatctggta atccggcgca cgataatcgt gacggtattt ggcttgtggc
     8101 tgttgagtca taaataacct ttagcatctt ttatagagtc tggtgttcag tctattcctg
     8161 ttgcgtaaat cgcgctatgc agaatcttca tcttttcagg tacaaacgcc tttattgcta
     8221 catttttata acatacaccg cgttatgcca tcgaccagaa aggtggcata tggtgtgatc
     8281 ggggttcaat aaattgcgaa acaaggtata ctccagcagt tcctgaagat gtttattgta
     8341 ctaaacgctc ctgtacgagg acgctactgc gcacctatga cacaattcgc ttctcctgtt
     8401 ctgcactcgt tgctggatac agatgcttat aagttgcata tgcagcaagc cgtgtttcat
     8461 cactattacg atgtgcatgt cgcggcggag tttcgttgcc gaggtgacga tctgctgggt
     8521 atttatgccg atgctattcg tgaacaggtt caggcgatgc agcacctgcg cctgcaggat
     8581 gatgaatatc agtggctctc tgccctgcct ttctttaagg ccgactatct taactggcta
     8641 cgcgagttcc gctttaaccc ggaacaagtc accgtgtcca acgataatgg caagctggat
     8701 attcgtttaa gcggcccgtg gcgtgaagtc atcctctggg aagttccttt gctggcggtt
     8761 atcagtgaaa tggtacatcg ctatcgctca ccgcaggccg acgttgcgca agccctcgac
     8821 acgctggaaa acaaattagt cgacttctcg gcattaaccg ccggtcttga tatgtcgcgc
     8881 ttccatctga tggattttgg cacccgccgc cgtttttctc gcgaagtaca agaaaccatc
     8941 gtgaaacgtc tgcaacagga atcctggttc gtgggcacca gcaactacga tctggcgcgt
     9001 cggctttccc tcacgccaat gggaacacag gcacacgaat ggttccaggc gcatcagcaa
     9061 atcagcccgg atctagccaa cagccagcga gctgcacttg ctgcctggct ggaagagtat
     9121 cccgaccaac ttggcattgc gttaaccgac tgcatcacca tggatgcttt cctgcgagat
     9181 ttcggcgtgg aatttgccag tcgttaccag ggcctgcgcc atgactctgg cgacccgctt
     9241 gaatggggtg aaaaagccat tgcacattat gaaaagctgg gaattgatcc acagagtaaa
     9301 acgctggttt tctctgacaa tctggattta cgcaaagcgg ttgagctata ccgccacttc
     9361 tcttcccgcg tgcaattaag ttttggtatt gggactcgcc tgacctgcga tatcccccag
     9421 gtaaaacctc tgaatattgt cattaagttg gtagagtgta acggtaaacc ggtggcgaaa
     9481 ctttctgaca gccctggcaa aacgatctgc cacgataaag cgtttgttcg ggcgctgcgc
     9541 aaagcgttcg accttccgca tattaaaaaa gccagttaat atcatcaggg agctaatcgg
     9601 ctcccttttt ttacctttaa ttccgaaatc tttcgctgca tttgcgaatt ctgcttgtct
     9661 gattgcagat gccaggtaac ataggtatcc ccccatttac gggtggacaa gtgtttattt
     9721 tttccgacta ttaacagaga gaatattatg agcgttgtgc ctgtagccga cgtactccag
     9781 ggccgcgtag ccgttgacag cgaagtcacc gtgcgcggat gggtacgtac ccgccgagat
     9841 tcaaaagctg gcatctcctt cctcgccgtt tatgacggtt cctgctttga tcctgtacag
     9901 gctgtcatca ataattctct gcccaattac aatgaagacg tcctgcgtct gaccaccggc
     9961 tgctcggtca ttgtaacggg taaagtcgtg gcgtcgccgg gccaggggca acaatttgaa
    10021 attcaggcca gcaaggttga agttgctggt tgggttgaag atccaaacac ttacccgatg
    10081 gcggcaaaac gccacagcat tgagtatctg cgtgaagtcg ctcacctgcg tccgcgcaca
    10141 aacctgattg gtgccgtcgc gcgcgttcgc catacgctgg cgcaggcgct gcatcgcttc
    10201 tttaacgagc agggattctt ctgggtttca acgccactga ttaccgcatc tgataccgaa
    10261 ggtgcaggcg aaatgttccg tgtttctacg ctggatctgg aaaacctgcc gcgtaacgat
    10321 cagggcaaag tggatttcga caaagacttc tttggtaaag agtctttcct gaccgtatct
    10381 ggccagttga acggcgaaac ctacgcttgc gcattgtcca aaatttatac cttcggcccg
    10441 actttccgtg ctgaaaactc caacaccagc cgtcacctgg cggaattctg gatgctggag
    10501 ccggaagtgg cgtttgctaa cctgaacgat atcgcaggtc tggctgaagc catgctgaaa
    10561 tatgtcttca aagcggttct cgaagaacgc gctgacgaca tgaaattctt cgctgaacgc
    10621 gtagataaag atgccgtttc acgtctggaa cgcttcattg aggccgattt cgcgcaggtg
    10681 gattacaccg acgcagtgac cattctcgaa aactgcggca ggaagtttga aaacccggtt
    10741 tactggggcg tcgatctctc ttctgagcat gagcgttatc tggcggaaga acactttaaa
    10801 gcaccagtag tggttaaaaa ctatccgaaa gatattaaag cgttctatat gcgccttaac
    10861 gaagacggta aaaccgttgc ggctatggac gttctggctc cgggcatcgg tgagatcatt
    10921 ggtggctccc agcgtgaaga acgtctggac gtgctggacg agcgtatgct tgaaatgggc
    10981 ctgaacaaag aagattactg gtggtatcgc gatctgcgtc gctacggtac tgttccgcat
    11041 tcaggtttcg gtcttggttt tgaacgcctg attgcttacg taactggcgt gcaaaacgta
    11101 cgtgatgtta ttccgttccc acgtactccg cgtaacgcca gcttctaatt tagtgtcttc
    11161 aagagccagc gcccgctggc tcttttttta tcctttctgt caagttatct gtttgttaag
    11221 tcaagcaatc tatttgcaac cctgccataa cgatcatcct gttacggaat attacactgc
    11281 aacatttacg cacaaaaaat aatctgcatt cttattgcgg attagttttt tcttagctga
    11341 tagcacaatt tttatacgat tttttggcat tctggatgtc tgaaagaaga ttttgtgccc
    11401 ggtcgataaa gtttccatca gaaacaaaat ttccg
//
LOCUS       DAPQZD010000153        11266 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPQZD010000153 DAPQZD010000000
VERSION     DAPQZD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.155"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..675
                     /gene="gspC"
                     /locus_tag="RO454_003940"
     CDS             <1..675
                     /gene="gspC"
                     /locus_tag="RO454_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDY0935696.1"
                     /translation="IINQPTNAFQSEFSLAALWRNENHTGVKDANPVAVNQETPKLSI
                     ALNGIVLTSNDETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYE
                     KVTLHPGLPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQ
                     PGDIALRINNLSLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQ
                     RNEGTQ"
     gene            659..2611
                     /gene="gspD"
                     /locus_tag="RO454_003941"
     CDS             659..2611
                     /gene="gspD"
                     /locus_tag="RO454_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDY0935697.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2621..4102
                     /gene="gspE"
                     /locus_tag="RO454_003942"
     CDS             2621..4102
                     /gene="gspE"
                     /locus_tag="RO454_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDY0935698.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4099..5295
                     /gene="gspF"
                     /locus_tag="RO454_003943"
     CDS             4099..5295
                     /gene="gspF"
                     /locus_tag="RO454_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDY0935699.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5305..5742
                     /gene="gspG"
                     /locus_tag="RO454_003944"
     CDS             5305..5742
                     /gene="gspG"
                     /locus_tag="RO454_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDY0935700.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5750..6259
                     /gene="gspH"
                     /locus_tag="RO454_003945"
     CDS             5750..6259
                     /gene="gspH"
                     /locus_tag="RO454_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDY0935701.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6256..6633
                     /gene="gspI"
                     /locus_tag="RO454_003946"
     CDS             6256..6633
                     /gene="gspI"
                     /locus_tag="RO454_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDY0935702.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6626..7213
                     /gene="gspJ"
                     /locus_tag="RO454_003947"
     CDS             6626..7213
                     /gene="gspJ"
                     /locus_tag="RO454_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDY0935703.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7206..8189
                     /gene="gspK"
                     /locus_tag="RO454_003948"
     CDS             7206..8189
                     /gene="gspK"
                     /locus_tag="RO454_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDY0935704.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8204..9367
                     /gene="gspL"
                     /locus_tag="RO454_003949"
     CDS             8204..9367
                     /gene="gspL"
                     /locus_tag="RO454_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDY0935705.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9364..9825
                     /gene="gspM"
                     /locus_tag="RO454_003950"
     CDS             9364..9825
                     /gene="gspM"
                     /locus_tag="RO454_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDY0935706.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9825..10497
                     /gene="gspO"
                     /locus_tag="RO454_003951"
                     /pseudo
     CDS             9825..10497
                     /gene="gspO"
                     /locus_tag="RO454_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
     gene            complement(10526..11002)
                     /gene="bfr"
                     /locus_tag="RO454_003952"
     CDS             complement(10526..11002)
                     /gene="bfr"
                     /locus_tag="RO454_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDY0935707.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11074..>11266)
                     /gene="bfd"
                     /locus_tag="RO454_003953"
     CDS             complement(11074..>11266)
                     /gene="bfd"
                     /locus_tag="RO454_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDY0935708.1"
                     /translation="YVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRA
                     AREVMEDELMQLPEFKESA"
BASE COUNT         2944 a         2897 c         2682 g         2743 t
ORIGIN      
        1 atcattaatc aaccaacaaa tgcatttcag tctgaattca gccttgccgc tttatggcgc
       61 aacgaaaatc atactggggt aaaagatgca aatcccgtgg cagttaatca ggaaacacct
      121 aagctctcta tagcgcttaa tggcattgta ctcacatcga atgatgagac ttcttttgtc
      181 ttaattaatg aggggaacga acaaaagcga tactccctca acgaagccct tgagtcagcg
      241 ccgggaacgt ttattagaaa aataaataaa accagcgttg tgtttgaaac tcacgggcat
      301 tacgaaaaag taactctcca tccagggcta cctgacatca tcaagcagcc tgattcggaa
      361 aatcaaaatg tactcgccga ttatattatc gccacaccta tacgtgatgg agaacagata
      421 tacggtcttc gattgaatcc tcgtaagggt ctcaatgcat ttaccaccag tttgctgcaa
      481 cctggggata tcgccctcag gatcaataat ctttcgctga cccaccctga tgagatttca
      541 caggcattaa gtttactgct aactcaacaa agtgctcagt ttacaattcg tcgcaacggc
      601 gtaccccgct tgataaatgt ttccgtcgcg gaacttacag gaatgaatgg acagcgcaat
      661 gaagggactc aataaaatca cctgctgctt gctggcagca ttactcatgc cttgtgcagg
      721 acacgctgag aacgaacaat acggcgctaa cttcaataac gccgatatcc gccagttcgt
      781 ggaaatagtg ggtcagcatc ttggcaaaac gatcctgatc gacccttcgg tacagggaac
      841 catttccgta cgcagtaatg atacgtttag ccaacaggag tactaccagt tctttttaag
      901 tattcttgat ctttacggtt attccgtgat cacgctggac aatggttttc tgaaagtggt
      961 gcgctcagct aatgtaaaaa catcgccagg gatgattgct gacagttctc gtccaggcgt
     1021 aggtgatgag ttggtcaccc gaatcgtacc gcttgagaac gttcctgctc gtgacctggc
     1081 tcccctgctc cgccagatga tggatgcggg tagcgtcggt aatgttgtgc attatgaacc
     1141 ctccaacgtt cttattctga ccggtcgtgc ctccaccatt aataaactga ttgaagtcat
     1201 aaagcgcgtt gatgtcatcg gcacagagaa gcagcaaatt attcatctgg aatatgcatc
     1261 agcggaagat ctcgccgaga ttcttaatca attaatcagc gaaagccacg gtaaaagcca
     1321 gatgcctgcc ctcctttccg cgaagattgt ggcggataag cgaaccaact ctcttatcat
     1381 cagcggaccg gaaaaagcac gccagcgcat aacttcatta ctgaaaagcc ttgatgtaga
     1441 agagagcgag gaaggaaata cccgggtcta ttacctgaaa tatgctaaag ccacgaatct
     1501 ggtggaagtg ctaaccggtg tttccgaaaa gctgaaagat gaaaaaggga attcgcgtaa
     1561 gccttcttct acttctgcga tggataatgt ggccattacc gccgatgaac agaccaactc
     1621 gttggtcatt accgctgacc agtccgtcca ggagaaactc gccacggtaa ttgcgcgtct
     1681 ggatattcgc cgtgcacagg tgctggttga ggcaatcatc gtcgaagttc aggatggaaa
     1741 tggactcaac ctcggcgtgc aatgggcgaa taaaaacgtt ggcgcacagc aatttaccaa
     1801 tacgggatta ccggttttta acgctgcgca aggtgtagct gattataaaa agaatggcgg
     1861 gatcaccagc gcgaatcctg cctgggatat gtttagcgcc tacaatggca tggctgcagg
     1921 cttcttcaat ggcgactggg gcgtattgtt gactgcgctg gccagtaaca ataaaaatga
     1981 catcctcgcc accccgagca tcgtaacgct ggataataaa ctcgcgtcct tcaacgttgg
     2041 tcaggatgtg ccggtgctat ccggctctca gaccacttct ggggataacg tctttaatac
     2101 tgtcgaacgc aaaacggtgg gcacaaaact caaagtcact ccgcaggtca atgaaggcga
     2161 cgcggtactg ctcgaaatag aacaggaggt ttccagcgtt gactcttcgt ctaattcgac
     2221 gctcggcccg acatttaaca cccgtactat tcaaaacgcc gtgctggtta aaaccggtga
     2281 aacggtggtc ctgggcggat tgctggatga tttttctaaa gagcaagtgt caaaggttcc
     2341 tctgcttggc gatattcctt tagtgggaca actcttccgc tatacctcga ccgagcgcgc
     2401 taaacgcaac ctgatggtat tcatccgccc gacgattatc cgtgacgatg atgtttatcg
     2461 ctcactgtca aaagagaaat acacccgcta ccgtcaggag caacagctgc gaatcgacgg
     2521 gaaatcaaaa gcgctggttg gctcggaaga tttgccggtg ctgaatgaga acacgttcaa
     2581 cagtcacgcc cctgcgccat cgtcacggtg aggcattctt atgagaattc actcaccgta
     2641 ccccgccagt tgggcgctgg cacaacgaat tggttatctc tattcagagg gcgagattat
     2701 ttatctcgcc gatacgccat tcgaacggtt actcgatatt caacgtcaag ttggtcagtg
     2761 ccagaccatg accagcttgt cacaggctga ttttgaagcc cgactggaag cggtgttcca
     2821 tcagaatacc ggtgagtcgc aacagattgc gcaggatatc gatcaatccg tcgatcttct
     2881 ctcgctttcg gaagagatgc ccgcaaatga agatctcctg aatgaagatt cagcggcacc
     2941 ggttatccgc ttgatcaatg ctattttgag tgaggccatc aaagaaaccg cctccgatat
     3001 ccacattgaa acctatgaaa aaacaatgtc gatccgtttt cgcatcgacg gcgttttgcg
     3061 gacaatttta cagcccaaca aaaaactggc ggcactgctt atctcccgaa ttaaggtcat
     3121 ggctcgtctt gatatcgccg aaaaacgtat tccacaggat ggaaggatca gtttgcgtat
     3181 cgggcgacgt aacatagatg tccgcgtatc cacactgcca tccatctatg gtgaacgcgc
     3241 tgtgctccgt ctgctggata aaaacagcct ccagctttca ttgaacaacc tggggatgac
     3301 ggcagcggat aaacaggatt tagaaaatct cattcagctt ccgcacggta ttatcctggt
     3361 gacagggccg acaggctccg gtaaaagcac cacgctctac gccatccttt cggcgcttaa
     3421 tatccctggc cgtaatattc tgacggtaga agatcccgtg gaatatgagc tggaaggcat
     3481 tggacaaacg caggtgaata cccgtgtgga tatgtctttc gctcgcggcc tgcgcgccat
     3541 acttcgccag gacccggatg tcgtcatggt gggggaaatt cgtgatacag aaaccgcgca
     3601 aattgcggtt caggcctcgc tcacaggcca tctggtactc tcaacactcc acactaacag
     3661 tgcatcaggc gcagtcaccc ggctacgcga catgggcgtc gaatcattcc tgctttcatc
     3721 ttcactggca gggattatcg cgcaacgtct ggttcgtcgt ctgtgtccgc aatgccgaca
     3781 attcacgccc gtatcgcccc agcaagcgca gatgtttaaa tatcatcagc tcgcggtgac
     3841 aacaattggc actcccgtag gttgccctca ttgccatcaa tccggttatc aggggcgcat
     3901 ggcgatccac gaaatgatgg tggtgacgcc ggaattgcgg gccgctattc atgaaaatgt
     3961 ggatgaacaa gcactggagc gactggtccg gcaacaacac aatgccttaa tcaaaaatgg
     4021 cctgcaaaca gtgatacgcg gtgacacctc ctgggatgag gttatgcgcg tcgccagtgc
     4081 tacgctggag aacaaagcat gaattatcgc tatcgcgcca tgacccagga tggtcaaaaa
     4141 ttgcagggga tcattgatgc taacgatgaa cgtcaggcac gactgcggct gcgtgaagaa
     4201 gggcttttcc tgttggatat tcgcccccaa aaaagttcgg gagtaaaaac acgtcgcccg
     4261 aggatcagcc atagtgaact gacgcttttc acccggcagt tggcaacctt aagcgcagcg
     4321 gcattacccc tggaagagag ccttgccgta attggtcaac aaagcagtaa taaacgactg
     4381 gctgacgtgt taaatcaggt acgcagcgcc atccttgaag ggcatcccct ttcagatgca
     4441 ttacagcatt tccccacgct tttcgattcg ctctaccgta ccctggtaaa agcgggcgaa
     4501 aagagcggtc tgctggcccc ggtgttggaa aagctggctg attacaatga aaaccggcag
     4561 aaaatccgca gcaagctcat tcagtcactg atctacccct gcatgctcac tacggtggcg
     4621 attgtggtcg tgattattct cctcactgct gtcgtgccca aaattaccga acagttcgtg
     4681 catatgaagc agcaactgcc actgagtaca cgcattcttt taggtctgag cgacacgttg
     4741 caacgtaccg gcccgacatt attagcgaca gtatttattg tcgctgtggg tttctggctc
     4801 tggttaaaac gcggcaataa ccgccaccgt tttcatgcca tgttactgcg cgttgcgctc
     4861 atcggcccgc tgatttgcgc cattaacagc gcacgctatc tccgcacttt aagtattttg
     4921 caatccagcg gcgtccctct gctggatggg atgaatttgt ccaccgaaag cctcaacaac
     4981 ctcgaaattc gccagcgcct ggcaaatgcg gcagaaaacg ttcgccaggg taacagcatt
     5041 catctttcac tggaacaaac cgcaattttc ccgccgatga tgctctacat ggtggcctct
     5101 ggcgaaaaaa gcgggcaact cggcacgtta atggtcagag ccgcagataa ccaggagaca
     5161 ctccaacaaa atcggatcgc cttaacgctc tccatcttcg agccagcact cattattacg
     5221 atggcactga tcgtcctgtt tattgtcgtg tcggtactcc aacctcttct tcaacttaac
     5281 tcaatgatta attaaggaaa tcatatgcgc gcaacggata agcaacgcgg ttttacatta
     5341 ctggaaatta tggtggtaat tgtcatcatt ggcgtactcg ccagcctggt ggttcccaac
     5401 ctgatgggca ataaagaaaa ggcggataaa caaaaagccg tcagcgatat tgtggcgctg
     5461 gaaaacgccc tggatatgta caagctcgac aatcatcgct atccaaccac aaatcagggg
     5521 cttgaatcct tagtagaagc gccgacactg ccaccgctgg ccgcaaacta taacaaggaa
     5581 ggttatatca agcgtctgcc tgccgatccc tggggcaatg attatgtcct cgttaatcct
     5641 ggtgaacatg gtgcatacga tctgctttca gcaggacccg atggtgaaat gggaaccgag
     5701 gacgacatca ccaactgggg tttgagcaag aagaaaaagt aagtcagcga tgaatcagca
     5761 acgcgggttt accctgctgg agatgatgct ggtgctggcg ctggtcgcta tcacggcaag
     5821 tgtggtgctc ttcacatatg gtcgggagga tgccgccagc actcgtgcgc gcgaaacagc
     5881 ggcgcggttt accgctgcac ttgaattagc catcgaccgc gccacgctca gtggtcagcc
     5941 tgtcggtatc catttctctg attcagcatg gcgcattatg gtaccgggca aaacaccatc
     6001 agcctggcgc tgggttccat tacaagaaga tgccgcagac gagagcaaaa atgactgggg
     6061 tgaagaactc tcaattcaac ttcagccatt taaacctgat gacagcaacc aaccgcaggt
     6121 ggtgattctg gccgacggac aaatcacgcc cttctctttg ttgatggcta ataccgggac
     6181 cggtgagccg ctcctgacgt tagtcagttc aggctcatgg cccctggatc aaacgctcgc
     6241 cagagatacc cgcccatgaa caaacaatca gggatgacac tgcttgaagt cttactggcg
     6301 atgagtattt tcaccgctgt tgcgctgact ttaatgagca gtatgcaagg gcaacgaact
     6361 gccatcgagc ggatgcgtaa cgagacgctg gcattgtgga tagcggataa ccagcttcag
     6421 tcacaagatt cattcgacga ggaaaacacg tccagttctg gcaaagagtt aataaatggc
     6481 gaagagtgga actggcgcag cgatatccac tcaagcaaag acggcacgct tctggaacgc
     6541 accataacgg tgacattacc cagcggccag acaacctcgc ttacgcgcta tcaaagcatc
     6601 aataataaat ccgggcaggc acaagatgat taatcgccag cagggtttca cattgctgga
     6661 agtcatggcg gcgctggcga ttttctcaat gcttagcgtg ctggcgttta tgattttttc
     6721 tcaagcttcc gagttgcatc aacgttcaca gaaagaaatc cagcagttca atcagctaca
     6781 gcgcaccatc accattcttg ataacgatct cctgcaactg gtcgcccggc ggaatcggag
     6841 tacggacaaa atcatggtgt tgggtgaaga agcgattttt accacgcaaa gtcgcgatcc
     6901 gctggcccct ctcagtgaag cacaaacctt actcactgtt cactggtatc tgcgaaatca
     6961 tacgctctac cgtgctgttc gtacttctgt ggatggcagg aaggatcaac ccgcccaggc
     7021 gatgctggaa catgtcgaga gctttcttct ggaaagtaac agcggagaaa gccaggagct
     7081 tcctctctca gtcacgttac atctgaagac tcagcaatat ggggcgttac agcgccgttt
     7141 tgcattacct gagcagttag cgagagaaga gtcgccagct cagacgcagg ccgggaataa
     7201 taaccatgaa taatgaacaa cgcggcgtag cactgctcat tgtactcatg ttactggcgc
     7261 tcatggctgc tctggctgca gacatgacga tcagttttca cggccagctt catcgaaccc
     7321 gccaggtaaa ccatcatttg caacggcaat atgatattga gctggcggag aaacttgcgc
     7381 ttgccagcct cacccaggac gttaaagata acgacagaca aaccacgttg cagcagtact
     7441 gggcacaacc gcaacagttg cagctggaaa atggcaatac agtgaagtgg cagttacgcg
     7501 atgcccagca ctgttttaat ctcaatgcct tagcaaaaat ctctgacgcc ccgctggctt
     7561 cccctgattt ccccgcccag gtattcagtg ccctgctgat taacgccggt atcgatcgcg
     7621 gaaacaccga tgagatcgtg caatcaatcg ctgactatat tgatgccgat gattcgccac
     7681 gctttcacgg cgcagaagat aacttttacc aaagccaaac gccgcctcgc catagcgcca
     7741 atcaaatgct ttttctgacc ggtgaattgc gtcagataaa aggcataacc gaaaacatct
     7801 atcagcggct tatcccgtat gtctgcgttc tcccgacctc ggaacttagc atcaatctta
     7861 atatgctaac ggagaacgat attccgctct tcagagcatt attcctcaat aacatcacgg
     7921 atgctgacgc tcgggtctta ttacaaaaaa ggcccaggga aggctggcta accacagatg
     7981 catttcttta ctgggcgcag caggatttct ccggcgtgaa gcccctggtt gctcaggtga
     8041 aagggcatct ctttccctat agccgctatt ttacgctgag tacggaaagt atcagcgacg
     8101 aacaatccca gggatggcaa agccatattt ttttcaaccg taaacagcag agcgcccaaa
     8161 tctatcggcg tacgctgcaa ctctactaat cataaggatc gtgatgcctg aatcgttgat
     8221 ggtaatacgt tctttctcga cgctgcgtaa gcattgggaa tggatgacgt tctccgcaga
     8281 tagcgtatct tcggtacata cgctcacaga cgatcttccg ctggggtcgc tggccgatca
     8341 gcccggtgcc ggaaatgttc accttttgat cccacctgag ggtcttttat atcgttccct
     8401 cacgctgccc aacgccaaat acaaactcac cgcgcaaaca ttacagtggt tggcggagga
     8461 gacattaccc gacgccagcc aaaattggca ctggacggtt gtcgataaac aaaacgaaag
     8521 tgtcgaggtg ataggcattc agtcggagaa actcagtcgt tatcttgagc gtttacatac
     8581 tgccggactc aacgtgacgc gggttttgcc agatggctgc tatcttccct gggaagtaga
     8641 tagctggact ctggttaacc agcaaacaag ctggcttatt cgcagtgccg cacatgcctt
     8701 caacgaactg gatgaacact ggttacagca tcttgctaac cagtttccgc ctgagaatat
     8761 gcgttgttat ggtgtagcgc ctcacggcgt tgcagcggcc aatccactta tccagcatcc
     8821 tgaaatacca tcactgagtt tgtattctgc cgatattgca ttccagcgct atgacatgct
     8881 gcatggtgtt tttcgtaaac agaaaactgt aggcaaatcg ggaaaatggc tcacccgcct
     8941 ggcagtaagc tgtctcgttc tcgccattct ttcttttgtt ggcagccggg gcatcgcgct
     9001 ctggcaaacg cttaagattg aagatcaact tcagcaacaa cagcaagaaa cctggcaacg
     9061 ttatttcccg cagatcaaac acacccacaa ctttcgtttt tactttaagc agcaactcgc
     9121 tcagcaatat cctgaagctg taccgttgct ctatcattta caaacacttc tgcttgaaca
     9181 ctctgaactg cagttaatgg aggcgaacta tagccagagg caaaagtcgc tgaccttaaa
     9241 aatgagtgct aagagtgaag ccaatatcga tcgtttctgt gagttaacac agtcctggct
     9301 tccgatggaa aaaacagaaa aagatccggt cagtggtgta tggacagtaa ggaactcagg
     9361 gaaatgatta aatcatggtg ggcagaaaaa tccacatcgg aaaaacagat cattgcggca
     9421 ctggcggttc tctcgctcgg cgttttttgt tggcttgcgg tgataaaacc gatagatacc
     9481 tacattgaag agcatcagtc ccacgcgcaa aaaattaaga aggacatcaa atggatgcag
     9541 gatcaagcga gcacccatgg tttactcggt catccagcgc ttacgcagcc catcaaaaat
     9601 atccttctcg aagaagcaaa acgtgagaat ttgactatca cgctggaaaa cggtccggac
     9661 aatactctga caattaatcc tgtcacagct cccctcgaaa acgtctcccg ctggctcact
     9721 acggcacaga taacatacgg tattgttatt gaagaccttc agtttactct tgccggaaac
     9781 gaagagatca ccctcaagca tttgtccttc agggagcaac aataatgaca atgttactgc
     9841 cgctcttcat tctggttggt tttattgcag gttattttgt taatgccatg gcctatcacc
     9901 tctcgccttt ggaggataaa acggcgttaa cgtttcgcca ggtattggtt catttctggc
     9961 aaaaaaaata tgcctggcat gatacagtgc ccctgatact ttgcgttgct gctgctatcg
    10021 cctgcgccct ggcacctttc acgcccatcg tgactggcgc actctttctc tatttctgtt
    10081 tcgcactcac gctcagtgtt attgattttc gcactcagct cctgcccgac aaactcacct
    10141 taccgctgct ctggcttggt atttaatgcg cagtctggat taattgattt acatgatgcg
    10201 gtttacggcg cggtcgctgg ctatggggtg ctgtggtgtg tttactgggg cgtctggtta
    10261 gtttgtcaca aagagggatt gggctacggt gatttcaagc tactggccgc tgcaggcgcg
    10321 tggtgtggct ggcaaacgtt gccaatgata ctgctgattg cctcgctggg tggcattggt
    10381 tacgccatcg tttcacaact tctgcaacgc cggactataa ccactatcgc attcggcccg
    10441 tggcttgcgc tcggcagcat gataaacctg ggatatctgg cctggatctc ttattaaccg
    10501 ggagggttct ccctcccgac acggctcaac cttcttcgcg aatctgtgct tgcagataat
    10561 tttgcagtcc catcttctga atcagatcaa gttccgtttc cagccagtcg atatggcctt
    10621 cttcatcgcg caaaatttct atcatcatat cgcggctgac gtaatcatga acgctatcgg
    10681 cataaccaat tgcctcacgc aaattcttcg cgccatccag ctcaagtgcc agatcagaac
    10741 gcagcatttc ctcaacatct tcaccaatgt tcagtttgcc caggtcctgt aagtttggaa
    10801 gaccttccag aaaaagaatg cgctctatat aacgatcggc gtgtttcatc tcatcaatgg
    10861 attcatgata ctccacatca ttgagacgtt tgagacccca gtttttaaac atccgggcat
    10921 gaagaaagta ctgattgatt gcgacaagct catttcccaa cagtttgttg agataattta
    10981 taactttagt atcacctttc attttttgac tccttcgctt ccactctttg aagcgtagaa
    11041 cggcttacga gtaagtcaaa aaacacactc cgtttatgcg gactccttaa actccggcag
    11101 ttgcattaat tcatcctcca tcacttcacg cgcggcccgg acacatttac cgcactgatt
    11161 gccgaccgga atgaattttt ttaattgctg gaacgagtgg ggggaaaact ggcgcacagc
    11221 ctgacgaatt tttttatcgc tgataccatt acaaagacaa acgtac
//
LOCUS       DAPQZD010000154        11172 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPQZD010000154 DAPQZD010000000
VERSION     DAPQZD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.156"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            94..198
                     /locus_tag="RO454_003954"
     CDS             94..198
                     /locus_tag="RO454_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDY0935709.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            508..2706
                     /gene="speF"
                     /locus_tag="RO454_003955"
     CDS             508..2706
                     /gene="speF"
                     /locus_tag="RO454_003955"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDY0935710.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2703..4022
                     /gene="potE"
                     /locus_tag="RO454_003956"
     CDS             2703..4022
                     /gene="potE"
                     /locus_tag="RO454_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDY0935711.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4079..5719)
                     /gene="pgm"
                     /locus_tag="RO454_003957"
     CDS             complement(4079..5719)
                     /gene="pgm"
                     /locus_tag="RO454_003957"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDY0935712.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5745..6290)
                     /gene="seqA"
                     /locus_tag="RO454_003958"
     CDS             complement(5745..6290)
                     /gene="seqA"
                     /locus_tag="RO454_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDY0935713.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6475..7239
                     /gene="ybfF"
                     /locus_tag="RO454_003959"
     CDS             6475..7239
                     /gene="ybfF"
                     /locus_tag="RO454_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDY0935714.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7379..7672
                     /gene="ybfE"
                     /locus_tag="RO454_003960"
     CDS             7379..7672
                     /gene="ybfE"
                     /locus_tag="RO454_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDY0935715.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7812..8342
                     /gene="fldA"
                     /locus_tag="RO454_003961"
     CDS             7812..8342
                     /gene="fldA"
                     /locus_tag="RO454_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDY0935716.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8552..8638
                     /gene="uof"
                     /locus_tag="RO454_003962"
     CDS             8552..8638
                     /gene="uof"
                     /locus_tag="RO454_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDY0935717.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8631..9077
                     /gene="fur"
                     /locus_tag="RO454_003963"
     CDS             8631..9077
                     /gene="fur"
                     /locus_tag="RO454_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDY0935718.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9161..9487)
                     /locus_tag="RO454_003964"
     CDS             complement(9161..9487)
                     /locus_tag="RO454_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935719.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9537..10943)
                     /gene="chiP"
                     /locus_tag="RO454_003965"
     CDS             complement(9537..10943)
                     /gene="chiP"
                     /locus_tag="RO454_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDY0935720.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2719 a         2779 c         2768 g         2906 t
ORIGIN      
        1 tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata gaataaccct
       61 accttcaact gacacagaaa aaggtaaagg taaatggaaa ataataaccg cttaatgcct
      121 catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt tgactttcat
      181 ttttttaatg cccgctagtc ttctgactaa agggcacccc agcgtacagg tctccctgac
      241 tttaagcatt acaggttaat acctgtatcc ctcggtgctc atatactgct aaccctttta
      301 aactctaaat aattcgagtc gcagcacatg caacttgaag tatgacgagt atagccagtt
      361 accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta tttatcagcg
      421 gtggggtttt gcttttttcc ggcgtgatca atttctcctt tgagaaattg aggacctgct
      481 attacctgaa ataaagagat gaacaaaatg tcagaattaa aaattgccgt tagccgttct
      541 tgcccggatt gtttttccac tcatcgtgaa tgcgtgaata ttgacaaaag taattatatt
      601 gacgttgccg ccattatttt atcagtcaat gatgttgaac atggaaaact cgatgaaata
      661 gacgctactg gctatggcat tcctgttttt attgcaacgg aaaatgaaga acgtgtacca
      721 gcagaatatc tgccacgtat ttctggtgtc ttcgagcatt gcgaatcacg gaaagagttt
      781 tatggacgtc agttagaaac cgctgccagc cattatgaaa cgcaactgcg cccgcccttc
      841 ttccgcgcac tggtcgatta tgtcaatcag ggcaatagcg cttttgactg cccgggtcat
      901 cagggaggtg agtttttccg tcgccatccg gcaggcaatc agttcgtgga atattttggc
      961 gaaatgctct tccgctccga cttgtgtaat gccgacgttg caatgggcga tctactgatt
     1021 catgaaggtg cgccatgcat tgcacagcaa catgcggcta aagtgtttaa tgccgataaa
     1081 acgtactttg ttttaaacgg cacatcctcc tccaacaaag tggttttaaa cgccctgctg
     1141 acaccgggtg atctggtgct gtttgaccgt aataaccata agtctaacca ccacggagcg
     1201 ttgctacagg ctggtgcaac accggtttat ctggaaacag cacgtaaccc gtatggtttt
     1261 atcggtggca ttgatgcgca ctgcttcgaa gaaagttatc tgcgtgaact gattacagaa
     1321 gttgcaccgc aacgggcaaa agaggcgcgt ccgttccgcc tcgctgtgat tcagttaggc
     1381 acctacgacg gtactattta taacgcccgc caggtggtgg ataaaatcgg ccatctgtgt
     1441 gactacatcc tgtttgactc cgcatgggtc ggctatgaac agtttattcc gatgatggcg
     1501 gactgttcgc cgctgctact ggagcttaat gagaacgatc cgggtattct ggttacgcaa
     1561 tctgtgcata aacaacaggc tggtttttct cagacttcac aaattcataa aaaagacagc
     1621 catatcaaag ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc ctttatgatg
     1681 cacgcctcca ccagcccgtt ctatccgctg tttgccgcac tggatatcaa cgccaaaatg
     1741 catgaaggtg tcagcggtcg taatatgtgg atggattgtg tggttaacgg tattaatgcc
     1801 cgcaaattga tcctcgataa ctgccagcac attcgcccgt tcgttcctga actggtggat
     1861 ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg cttctttaaa
     1921 tttgtacccg gtgagcactg gcattctttt gaaggctacg cagagaacca atactttgtc
     1981 gatccgtgca aactgttgct gacaactcca ggtattgatg cacgtaacgg cgaatatgaa
     2041 gcgttcggtg tacccgcaac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg
     2101 gaaaaatgcg atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaaa
     2161 ctgcagcaac ttgttgccct gctggtacgc ttcgaaaaac tactggaagc tgatgcgcct
     2221 ctggcggaag tactgccttc catctacaaa cagcatgaag agcgctacgc cggttatacc
     2281 ctgcgtcagt tgtgccagga aatgcatgat ttgtatgccc gtcacaacgt gaaacaactg
     2341 caaaaagaga tgttccgtaa ggaacacttc cctcgcgtca gcatgaatcc gcaagaagcc
     2401 aactacgcct acttacgcgg tgaagtggag ctggttcgtc tgccggatgc agaaggccgt
     2461 atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt tcctggtgaa
     2521 atctggggtg gtgctgttct gcgttacttc agcgctctgg aagaaggcat caacctgctg
     2581 ccaggttttg caccggagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa
     2641 gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagcg ccctgttgaa aggggaaaaa
     2701 ttatgagtca ggcgaaatcg aacaaaatgg gtgtcgttca gttaaccata ctgacgatgg
     2761 tcaacatgat gggctccggt atcatcatgc tgccgacaaa gcttgccgaa gtcgggacaa
     2821 tctcaattat ctcctggctg gtgacagccg taggctcaat ggcactggca tgggcattcg
     2881 caaaatgcgg tatgttcagc cgtaaatcag gaggtatggg cggttatgcc gagtatgctt
     2941 tcggtaaatc cggtaacttt atggcgaact atacctacgg cgtctcgctg ctgattgcta
     3001 acgtcgcgat tgctatttcg gcggttggtt acggcaccga actgctcggc gcgagtttgt
     3061 cgccagtgca gattggtctt gcgaccatcg gggtgctgtg gatttgtacc gtggctaact
     3121 ttggtggtgc gcgcattacc gggcaaatca gtagcattac cgtgtggggg gtcattattc
     3181 cggtggtagg cctgtgcatt atcggctggt tctggtttag cccgacgctt tacgttgatt
     3241 cctggaatcc gcatcatgca cccttcttca gtgcggtagg ttcttccatc gctatgacgc
     3301 tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac tgatgtagtg gaaaacccgg
     3361 aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg atttatatcg
     3421 tctccaccaa cgtgattgcc gggattgtgc caaatatgga gctggcaaat tcaacggcac
     3481 catttggtct ggccttcgcg cagatgttca cgccggaagt gggtaaagtc attatggcgc
     3541 tgatggtgat gtcctgctgc ggttcgctgc ttggctggca gttcaccatt gcccaggtgt
     3601 ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta accaaagtgg
     3661 atgcaccggt gcagggaatg ttgaccattg tgattatcca gagcgggttg gcgctgatga
     3721 cgattagccc gtcgctaaac agtcagttca acgtgctggt taacctggcc gtggtgacta
     3781 atatcattcc gtatattctg tcaatggcgg cattagtcat tattcagaag gtcgctaatg
     3841 tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg atgtacagct
     3901 tttatgcgct gtactcatcc ggggaagaag ccatgctata cggttccatc gtgaccttcc
     3961 tcggttggac actgtatggt ctggtctcac cacgctttga actgaaaaat aaacacggtt
     4021 aattaagcaa agcaaaaaag ggcgatcttc cgaccgccct ttttcttatt tgatatgttt
     4081 acgcgttttt cagaacttcg ctaacaattt caaccgcttc tttctcaatc tgcttgcgat
     4141 gttcttcacc aaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
     4201 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccgatagaa gcgccattac
     4261 ccggtgccgc cgtcagacgc gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca
     4321 tttccggaga cagcttagac aacgccgctt tttgtgcgga agtcgctgct gcctgcaaac
     4381 ggttatagct cggcgcacca aagcgttttg ccagttcgtt atagtgttcc tgcgggttct
     4441 taccggtgac agcggtgatt tccgccgcca gcagacacat aatgatgccg tctttatcgg
     4501 tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa
     4561 agccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc gggacttcta
     4621 ccagcttacg gcccaagtca ttgaccacac ggtcgatcat cgcagatgaa accagcgttt
     4681 taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtaa ttgatcgcca
     4741 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
     4801 aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc aggcccgcca
     4861 tcgcacactc ggaggagcag tccatacgga tcgcgccgtc tttatcaagg tgcataaagc
     4921 ggaaggtttg atcgacctga tcgttaacaa tagtcaggtt gaggttgtaa tactcgccaa
     4981 tacgcttcca gtattcgata ccggaaccgc ccagcggatc aacgccaagc gtcaggcccg
     5041 ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacaaac ggctgcacca
     5101 gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
     5161 ctttcaggcc atcagccagc agtgcgtttg ctctgtcttc caccacttta gtgacgttgg
     5221 tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat
     5281 gggacggtgt aatcacgata ccgtctgcca gcgggccacc ttttttattg tgaaccagaa
     5341 tggcattgga aatggcaggt gtcggagtga agccattgtt ttcctgcaca atgacatcaa
     5401 cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag
     5461 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
     5521 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
     5581 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
     5641 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
     5701 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
     5761 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
     5821 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtaccagg cacatgtttc
     5881 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt
     5941 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
     6001 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa
     6061 agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg
     6121 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
     6181 gcggtaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
     6241 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
     6301 tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc ggcagcggat
     6361 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
     6421 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
     6481 ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
     6541 catggtctgt ttggcagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
     6601 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga tccggtaatg
     6661 aattacccgg cgatggcgca ggatcttgtt gataccctgg atgcacagca gatcgacaaa
     6721 gcaacattta tcggtcactc catgggcggt aaagcggtaa tggctcttac tgcactagcc
     6781 cctgatcgca ttgacaagct ggtggcgatt gatattgcgc cggtcgatta tcacgttcgc
     6841 cgtcatgatg agattttcgc ggccatcaac gcagtcagtg aatcagacgc acaaacacgc
     6901 cagcaagcag cggcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg
     6961 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cagtattgtg ggatcagtat
     7021 ccgcatattg tgggttggga gaaaatcccg gcatgggatc atcctaccct gtttatccct
     7081 ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
     7141 caggctcggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
     7201 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
     7261 cgtccgcgtc gctttccgcg cgtggacgtt ggcgtgactc atcgcgctga tgtattatgg
     7321 cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat
     7381 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
     7441 acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca
     7501 actaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc
     7561 tgtcgaggcg ctgaacgagc tggcggaatc gcgtaatatg agccgtagcg aactgatcga
     7621 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
     7681 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
     7741 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
     7801 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
     7861 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
     7921 caaaaagcag caaagaagat ctggaagcat atgacattct gctgctgggc atcccaacct
     7981 ggtattacgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
     8041 attttaatgg taaactggtt gcgctgtttg gttgtggcga ccaggaagat tacgcagaat
     8101 acttctgcga tgcattgggc accatccgcg acatcattga accgcgcggc gcaactatcg
     8161 ttggtcactg gccaaccgcg ggctaccact tcgaagcgtc aaaaggtctg gctgatgatg
     8221 accactttgt cggtctggct atcgacgaag atcgtcagcc ggaactgacc gctgaacgtg
     8281 tagaaaaatg ggttaaacaa atttctgaag agctgcatct cgacgaaatt ctcaatgcct
     8341 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
     8401 attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt gttcaagtgg
     8461 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
     8521 gtagttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
     8581 tgtcacttct tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
     8641 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg
     8701 aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
     8761 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
     8821 acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
     8881 agcaacatca ccacgatcac ctgatctgcc tcgactgcgg taaggttatc gaatttagtg
     8941 atgattccat cgaagcgcgt cagcgtgaaa tcgccgcaaa acatggcatt cgcctgacta
     9001 accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgagcacg
     9061 cgcacgaagg caaataagcc agcctgaacg agaaaagcca acctgcaggt tggctttttt
     9121 atacaaggga aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgaa
     9181 tttcctgcca gaccttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg
     9241 cctgaatgca ctgctgataa tccagtacgc ggacactttc ctgctcggca aactgctgat
     9301 gttgtttctc tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttccggcg
     9361 aaccttgcgc ggtgttgata caggcgctgt aagcctcttt cagacgactg tcttcctgtg
     9421 gcgcggttga ttgcgcacaa gccaccagcc ccgatgccat catggcgatg agaatcagtt
     9481 ttttcatagc aaattcctca aacgtgcggc aggcacaaaa cctgccgcat cgggcatcag
     9541 aagatggtga atggtgcgat taccataaat ttcacgtcac gctcatcctg gaagatgttg
     9601 ccataaccac cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt
     9661 ttgaacatcg tgcctttggc gcggccgtcc tgaatggtgt agaccgcatc caggctgtag
     9721 gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgccaggta
     9781 gcgggtttag catcccatgc gtaaacgtag gaggcgccga tggcaaaacc tggaagattc
     9841 cagtttttca ggtcatacat cgcgccgaag aagaccgctt tttcgccgtt ggcgttgaag
     9901 tcagaacggt tatcccacca gatatccagg cgaccgttcg aggaagcgta ggttggtgtc
     9961 atacgttgca ggaagtatcc ctgctgaccg tcggctttaa cccaggtgcc ttccagacgc
    10021 aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cctgcagcca ggcggtgccg
    10081 tcataaagat cgttggcgct gcgatcgtca actttatcgc gcgtgccata gaactggtag
    10141 ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc aaaatattga
    10201 tcgatatacc cttcggcctg accaaacgcc gcttccagta cgaagttatt tttgaagtcg
    10261 tacttcgccc caagggagtg tagataatca actttggtgg ttttatcgtt ctgataaaac
    10321 tcatccattt ccagatgcca cggtgctttg tattcgttgg tccacatgta ggagaaactc
    10381 aacgcaccag catcgccgta atcaaaattc gccccggcct ccgccccctg ataagtaccc
    10441 ggcataaagc tccagtgtgg cgctaacagc gtctgaccgg ttggctgaat ataacctgcc
    10501 ctcgcccaaa ccggaccgta tttaaatttg gccgctgctt tatacaggct tataccgctt
    10561 ttatcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc gatttcgttc
    10621 gggtgagagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcggc aatatcaagg
    10681 ccgaacatat cagcagcata accagactga aaatcgaggt tggcgttcca ggtggagtga
    10741 gaaaggttgg ttttgtattt atcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc
    10801 cagtaataga taccgccggt taaggttgaa tcgtcgatga atccttcggc gcgagcctcc
    10861 ggaattgcca taaagcccga catagctgta acaccggcga tagccagcgc cagcgtacta
    10921 cgtttgccac taaacgcacg catgggttaa tcctctttga cgtataaatt gctgcaccaa
    10981 aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    11041 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    11101 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcagtt
    11161 gaaaggcttg ca
//
LOCUS       DAPQZD010000155        11154 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPQZD010000155 DAPQZD010000000
VERSION     DAPQZD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.157"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            23..322
                     /locus_tag="RO454_003966"
     CDS             23..322
                     /locus_tag="RO454_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDY0935721.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(442..621)
                     /locus_tag="RO454_003967"
     CDS             complement(442..621)
                     /locus_tag="RO454_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDY0935722.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            695..2056
                     /gene="pabB"
                     /locus_tag="RO454_003968"
     CDS             695..2056
                     /gene="pabB"
                     /locus_tag="RO454_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDY0935723.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSHLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLLEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2060..2638
                     /locus_tag="RO454_003969"
     CDS             2060..2638
                     /locus_tag="RO454_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDY0935724.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEGEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2822..4186
                     /gene="sdaA"
                     /locus_tag="RO454_003970"
     CDS             2822..4186
                     /gene="sdaA"
                     /locus_tag="RO454_003970"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDY0935725.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4317..5915
                     /locus_tag="RO454_003971"
     CDS             4317..5915
                     /locus_tag="RO454_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDY0935726.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(5919..7475)
                     /gene="yoaE"
                     /locus_tag="RO454_003972"
     CDS             complement(5919..7475)
                     /gene="yoaE"
                     /locus_tag="RO454_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDY0935727.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7939..8910
                     /gene="manX"
                     /locus_tag="RO454_003973"
     CDS             7939..8910
                     /gene="manX"
                     /locus_tag="RO454_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDY0935728.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8973..9773
                     /gene="manY"
                     /locus_tag="RO454_003974"
     CDS             8973..9773
                     /gene="manY"
                     /locus_tag="RO454_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDY0935729.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9786..10637
                     /gene="manZ"
                     /locus_tag="RO454_003975"
     CDS             9786..10637
                     /gene="manZ"
                     /locus_tag="RO454_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDY0935730.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10692..11150
                     /locus_tag="RO454_003976"
     CDS             10692..11150
                     /locus_tag="RO454_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDY0935731.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2722 a         2828 c         2820 g         2784 t
ORIGIN      
        1 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
       61 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagtacgg cgaagggaat
      121 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      181 tgaccaggaa ggctggaacc ctggtttcac ggaaaaaatg gttggatggg caaaaaaaat
      241 ggagtctggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      301 agaactaaaa gcactggttt gagttaaatt cacatcagca taaatgggtc aggacgcttt
      361 taatcacata taaaaagcgt cctttttctc acaatcaaat aatcatcggc ttctataacg
      421 aaagcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattcgctc
      481 gccggaatgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctgctcat
      541 cccttgtgcc atcagttcct ggatccgctc gacggctttt tgctgctgtt catgggtgag
      601 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      661 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      721 tactttaccc tggcgtcagg acgccgctga attttatttc tcccacttaa gccacctgcc
      781 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      841 ggtcgccgag ccaatttgca ctttaaccac tttcggtaaa gaaaccgttg ttagtgaaag
      901 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
      961 tgcagacatt cgcccagcgc ataacgaaga tttgccattt cagggcggcg cgctggggtt
     1021 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1081 cgttctgccg gatatggcag tgggtatcta cgactgggcg ctggttgtcg accaccagcg
     1141 tcaaacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1201 ccagcaattc tccccgcagg aagatttcac actcacttcc gactggcaat ccaatatgac
     1261 ccgcgaacag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1321 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1381 attccttcag cttaatcagg ccaaccgcgc gccgtttagc gcttttttac gtcttgaaca
     1441 ggctgctatt ttaagccttt caccagagcg gtttattctt tgtgataatg gtgaaatcca
     1501 gacccgcccg attaaaggca cgctaccacg cctgcccgat cttctggaag atagcaaaca
     1561 agcagaaaaa ctggcgaact caacgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1621 aatgcgtaat gatatcggtc gtgttgccgt agcaggttcg gtaaaagtac cagagctgtt
     1681 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc accataacgg cgcaactacc
     1741 agaacagtta cacgccagcg atctgctgcg cgcggctttt cctggtggct caataaccgg
     1801 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgcaatgc
     1861 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggacacca gcattactat
     1921 ccgcacgctg actaccatta acggacaaat ttactgctct gcgggcggtg gaattgttgc
     1981 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2041 ccaactggag aagtaagacg tggaataccg tagcctgacg cttgatgatt ttttatcgcg
     2101 ctttcaactt ttgcgcccgc aaattaaccg ggaaacctta aatcatcgtc aggctgccgt
     2161 gttaatcccc atcgtccgcc gaccgcaacc ggggttgttg ctgactcagc gttcgattca
     2221 tctgcgtaaa cacgctggac aagtggcatt ccctggaggc gcagtcgatg acacggacgc
     2281 atcagttatc gccgctgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2341 cgttgaggtt atcggcgtgc tgccgcccgt cgatagcgtc actggctacc aggtaacccc
     2401 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaaggtg aagtctccgc
     2461 cgtctttgaa atgccgctcg cccaggcatt acatctgggt cgttatcatc ctttagatat
     2521 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2581 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga aaccctgact
     2641 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2701 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2761 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2821 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     2881 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     2941 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3001 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3061 cgacagtatt cccggtttta ttcgcgacgt agaagagcgc gaacgtctgc tgctggcaca
     3121 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggtaacct
     3181 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3241 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3301 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3361 ctgtaatgaa accggctttt cgctgtctgg tctcgctatg caaaacgaac tggcgctgca
     3421 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3481 cgatcgcggg atgaataccg aaggcgtact gccaggcccg ttgcgcgtgc cacgtcgtgc
     3541 gtcggccctg cgccggatgc tggtttccag cgataaactg tctaacgatc cgatgaatgt
     3601 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggccg
     3661 tgtggtaact gcgccaacta acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3721 tgaccacttt attgaatcag ttagcccaga catctatacc cgttacttta tggcagcggg
     3781 cgcgattggt gcactgtata aaatgaacgc gtctatttcc ggtgcggaag tcggttgcca
     3841 gggcgaagta ggtgttgcct gttcaatggc tgctgcgggc cttgccgagc tgttgggcgg
     3901 tagcccggaa caggtttgtg tggcagcgga aattggcatg gaacacaacc tcggtctgac
     3961 ctgcgacccc gttgcagggc aagttcaggt gccgtgcatt gaacgtaatg ccattgcctc
     4021 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4081 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ctaaataccg
     4141 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttattcgc
     4201 ccatctgcaa cggatgggcg aatttattcc cgctttctcg tctgctgtaa ttttccccac
     4261 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4321 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4381 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4441 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4501 ttcccctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4561 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4621 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4681 acattctggc tgaacttcct gcaccgcaac cacttttacg actgacgacc gaccgtgccc
     4741 tgataaaagg cagtccggtt ttgattcaat ggacgcctgc agcgggcagt agcaatgctg
     4801 gggtcatgga gatgattaac atcgacttac tgacggcaat gctccttgaa ccacaactgc
     4861 cgcaaatcag tagcgccagc ctgacggtgg acagtcggca tttgctctat ggtaatgggc
     4921 tggtagattc ccttccgcaa cctgaaaaca atgaaaacta tcaggtttct tcgcaacgct
     4981 ttccttttac cattaacgtt aatggcccgg gggctacggc gctggcatgg cactatcttc
     5041 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctacatc gcctggctgg
     5101 cgaccgctta ccgaatgagc ttttcccgcg agatcaatct gggcctggcg caacatgagt
     5161 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5221 ttttgctgcg ctggaacaat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5281 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5341 gtcagcgcca tgttttccca atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5401 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5461 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattacc
     5521 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5581 gcaacagttc attttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5641 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5701 cactggggca aagactgaat attgaactgg tggcggaggg cgtagaaacg caagaacagg
     5761 cgaagtattt gcgcagtcat ggcgtgcata ttttgcaagg gtatttgtac gcacagccga
     5821 tgccgctacg tgattttccc aaatggctgg cgggcagcca accgccgccc gcccggcata
     5881 atggacatat cacgcccgtt atgccgttac gttaacgctt actcatcttc atcgtgcgcc
     5941 ggttgctctt taacaatgcg aaccagatca acacgataat cattggcttc aatgatggtg
     6001 atatgcagtg gacctacatc aatcacatcg cccacacggg gaatgtgacc atttgccgag
     6061 atcacgaggc ccgcgaccgt cgcgatatca tcgtcatcgg caaggtgctc aacatcaagc
     6121 gcctgctgca aggcatgcaa atctgtaccg ccttttacca gccagccgtc accgtcggta
     6181 atgatttccg gcgtttcgtc agcgtccggg aattcacccg caatggcttc cagcacatcc
     6241 agcggcgtga ccagaccttg taccacacca aactcgttgg tcacgataac aaagctcccg
     6301 cgagcacgac gcagcacgcc cagcaggttg atcggatcga gggtttccgg gacgataatc
     6361 gccggagacg ccgaagctat cgccgccaca tcaactccct cttccagcgc caccagcagt
     6421 tctttagcac gtacgatgcc gataatttca tccagttcac cgcgacatac cgggaacaaa
     6481 ctgtgcggtg aggagagcag ttgctcgcgg atttcatcga ccccgagatt agcatcaacc
     6541 cagcttattt caccgcgcgg cgtcatgatc ccgcgcagag aacgcgacgc cagcgtcagt
     6601 acgccgttaa tcatgtaacg ttcttcttcg gcaaacgcac cttccgggat cggcatcggc
     6661 atcgggttat cggcatcgtg ctgaacattg gcctgacgtt tcccgcccat caaacgcagg
     6721 atggcatcgg cagtacgcgc tcgcagcggc aaagtcgact ggtgacgaat aaagttgcga
     6781 cgcgcaatct ggttaaacac ttcgatgatg atcgagaagc caatcgcggc atacaggtaa
     6841 cctttcggaa tgtggaaacc gaaaccttct gccaccagac tcagaccaat cattaacagg
     6901 aagctcagac agagcaccac caccgtgggg tgctggttaa caaatcgcgt cagcggtttc
     6961 gatgccagca gcataaccgc catcgcaatc actaccgccg ccatcatcac cggcaaatgg
     7021 ttaaccatcc ctactgcagt aattaccgca tccaacgaaa agacggcgtc aaggatgaca
     7081 atctgtgtga cgaccaccca gaaactggcg tagcctttac cgtggccgga atcatgatcg
     7141 cggttttcca gccgttcatg cagttcggtt gttgctttga acagcaagaa tatgcccccg
     7201 aacaacataa tcaggtcgcg tccggagaag gagaaatcca tgacggtaaa tagcggtttg
     7261 gtcagcgtga ccatccatga aatcagcgac agcagcccca gacgcataat cagcgccagt
     7321 gataatccca gcaaacgcgc tttatcgcgt tgttttggcg gcagtttgtc agcaagaatg
     7381 gcgatgaaga ccaggttatc gatacccagc acaatttcga gaacaacaag cgtgagtagc
     7441 cccgcccaaa tcgaggggtc cattaagaat tccatgacaa gctcctgctt aaggaatagc
     7501 tattcgacgc cagaaataat gcaggcgtaa cgacaaaatg caaacgaaag gtgcggcata
     7561 aagtgccaga aagacaggcg ttaaaaggcc tgatgctgaa atgacgtcgg tgacgatcca
     7621 tactgcgggc tactgcccta tactccatgg ttgttaaaca ggagttaaac atatcagaga
     7681 cgcctctgat ttggcaaaga tttaccttcc tttgcaaacg aatgtgacaa ggatatttta
     7741 cctttcgaaa tttctgctaa tcgaaagtta aattacggat cttcatcaca taaaataatt
     7801 ttttcgatat ctaaaataaa tcgcgaaacg cagggggttt ttggttgtag cccttatctg
     7861 aatcgattcg attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga
     7921 taataaagga ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca
     7981 gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc
     8041 gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca
     8101 aaactcgaca ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccattc
     8161 aatgctgcca gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac
     8221 attccaatgc tcgtggaaac gttaatggcc cgagatgatg accctagctt tgatgaactg
     8281 gtggcactgg cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccgatt
     8341 gaaaaagccg cgccagcacc cgctgccgca gcacccaaag tggctccaac tccggcaaaa
     8401 ccaatgggac cgaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt
     8461 cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt
     8521 agtgatgaag tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg
     8581 ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa
     8641 tatgctggcg aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt
     8701 gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa
     8761 acccaggtga ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caagaaactg
     8821 aatgcgcgcg gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg
     8881 atggatctga tcagcaaaat cgataagtaa cgtattgtgt tgattatcac tcagttttca
     8941 cacttaagtc ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc
     9001 tggtatttat cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc
     9061 accgtccgct aatcgcgtgt accctggtgg gtatcgttct tggcgatatg aaaaccggta
     9121 ttattatcgg tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgccg
     9181 ttgcgcctga cgccgctctg gcttccatca tttctactat tctggttatc gcaggtcatc
     9241 agagcattgg tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga
     9301 ccatcatcgt tcgtactatc accgttgctt tccagcacgc tgcggataag gctgctgata
     9361 acggcaacct gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc
     9421 gtgtggctat tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtccagaaca
     9481 tgctgaatgc gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg
     9541 tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct
     9601 tctacctcgg cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga
     9661 ttggtactgt tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg
     9721 gtgcgcctgc tcaggctgct ggtaacaacg atctcgataa cgaactggac taacaggtga
     9781 gcgaaatggt tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc
     9841 gtggcgtctt cctgcgttct aacctctttc agggttcatg gaacttcgaa cgtatgcagg
     9901 cactgggttt ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg
     9961 aagctcgtaa acaagctatt cgccgtcacc tggagttctt taacacccag ccattcgtgg
    10021 ctgcgccgat tctcggcgta accctggcac tggaagaaca gcgtgctaat ggcgcagaga
    10081 tcgatgacgg tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag
    10141 gcgacccgat cttctgggga acggtacgtc cggtatttgc agcgctgggg gctggtatcg
    10201 cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc
    10261 tggcaacccg ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag
    10321 atatgggtgg tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg
    10381 tcatgggggc attggttaat aagtggacac atgtcaacat cccgctggtg gtctctcgca
    10441 ttactgacca gacgggcaaa gaacacgtta ccactgtcca gactattctg gaccagttaa
    10501 tgccaggcct ggtaccactg ctgctgacct ttgcttgtat gtggctgttg cgcaaaaaag
    10561 ttaacccgct gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg
    10621 gcctgctggg actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat
    10681 ctggaggatt aatgacaatc acggacctgg tactgattct tttcatcgcc gcacttctgg
    10741 ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa
    10801 ttcctttgct ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc
    10861 ttatctataa caacgtcacg aatcatggcg cgcttataac aacatggtta ttaagtgcac
    10921 tggctctgat gggtttttat atattctgga ttcgcgttcc gaagatcatc tttaaacaaa
    10981 aaggtttttt cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt
    11041 cggaagatgg cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa
    11101 atatcgacga tttggaaaaa atttataagc ttctcgtttc aactcaataa gtta
//
LOCUS       DAPQZD010000156        11130 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPQZD010000156 DAPQZD010000000
VERSION     DAPQZD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.158"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            26..1597
                     /gene="garD"
                     /locus_tag="RO454_003977"
     CDS             26..1597
                     /gene="garD"
                     /locus_tag="RO454_003977"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDY0935732.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1746..2081
                     /gene="prlF"
                     /locus_tag="RO454_003978"
     CDS             1746..2081
                     /gene="prlF"
                     /locus_tag="RO454_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDY0935733.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2081..2545
                     /gene="yhaV"
                     /locus_tag="RO454_003979"
     CDS             2081..2545
                     /gene="yhaV"
                     /locus_tag="RO454_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDY0935734.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2600..3409)
                     /gene="agaR"
                     /locus_tag="RO454_003980"
     CDS             complement(2600..3409)
                     /gene="agaR"
                     /locus_tag="RO454_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDY0935735.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3658..4938
                     /gene="kbaZ"
                     /locus_tag="RO454_003981"
     CDS             3658..4938
                     /gene="kbaZ"
                     /locus_tag="RO454_003981"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDY0935736.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4961..5434
                     /gene="agaV"
                     /locus_tag="RO454_003982"
     CDS             4961..5434
                     /gene="agaV"
                     /locus_tag="RO454_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDY0935737.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5445..6224
                     /locus_tag="RO454_003983"
     CDS             5445..6224
                     /locus_tag="RO454_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDY0935738.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6214..7092
                     /locus_tag="RO454_003984"
     CDS             6214..7092
                     /locus_tag="RO454_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDY0935739.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7110..7544
                     /locus_tag="RO454_003985"
     CDS             7110..7544
                     /locus_tag="RO454_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDY0935740.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7541..8674
                     /gene="nagA"
                     /locus_tag="RO454_003986"
     CDS             7541..8674
                     /gene="nagA"
                     /locus_tag="RO454_003986"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDY0935741.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9024..10178
                     /locus_tag="RO454_003987"
     CDS             9024..10178
                     /locus_tag="RO454_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDY0935742.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            10191..11051
                     /gene="kbaY"
                     /locus_tag="RO454_003988"
     CDS             10191..11051
                     /gene="kbaY"
                     /locus_tag="RO454_003988"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDY0935743.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2656 a         2883 c         2973 g         2618 t
ORIGIN      
        1 gtccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac
       61 tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg
      121 cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg
      181 gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt atggcgaggt
      241 gattggttac gccgtgcgcg caatcccacg cggaagctgg atcgacgaat caatggttgt
      301 actaccggaa gcgccgccgt tacatacgct gccactggca accaaagtcc cggaaccctt
      361 accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac
      421 caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt
      481 agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg
      541 gctgaatcat ttgtacggtt gtggcgtggc gattaacgca cccgcggcag ttgtacctat
      601 tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa tggtgattgg
      661 tctgggttgt gaaaagttgc agcctgaacg cctactgact ggaacggatg atgtgcaagc
      721 tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggttttca
      781 gtccatggtc gaggatattt tgcaggtagc cgaacgccat ctacaaaaac tgaatcaacg
      841 tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg gtggcagcga
      901 tgcgttttct ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tattggtgcg
      961 ctgcggcgca acggtgatgt tttcagaagt cacagaagtg cgtgacgcga tccatctgct
     1021 gacaccacgc gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta
     1081 cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa
     1141 gaaaggcggt ctggcaaacg tggtagagaa ggcactcggc tccattgcta aatcgggtaa
     1201 aagtgcaatt gttgaagtgc tgtcgcccgg ccaacgcccg actaaacgcg gattaattta
     1261 cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac
     1321 cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc cttatggcgg tacccgtcat
     1381 taaaatggcg acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc
     1441 gggcactatc gctaccggcg aagaaaccat tgaagaagtg ggctggaagt tgttccactt
     1501 tattctcgac gtcgccagcg ggaagaagaa aactttctcg gatcaatggg gactgcataa
     1561 ccagctggcg gtgtttaacc cggcaccggt gacctgattt cttttggaac gtccctcgca
     1621 aaacatggcc ttagtgccat gtttttattg tttaaagtcc acacatccat taataatgca
     1681 tttgcattac cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt
     1741 aaataatgcc cgctaatgct cgctctaacg ctgtactgac cactgaatca aaggtcacta
     1801 tacgcggaca aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc
     1861 tggacagcat tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag
     1921 atgaacagga ggatcatact atgaatgcat ttttgcgttt tctggatgca gatatccaga
     1981 acaatccgca aaaaactcgt ccattcgaca ttcaacaagg aaagaaactt gtcgctggca
     2041 tggacgtcaa cattgacgat gagattggcg acgacgaata atggattttc cacaaagggt
     2101 taatggttgg gcgctatatg ctcatccctg ttttcaggaa acctacgatg ctttagttgc
     2161 cgaagtcgag gcattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa
     2221 gttattggcg gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc
     2281 ggcattccgt catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa
     2341 atttggtgct ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat
     2401 tctgggatgg atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta
     2461 taccgtattc agtaaaatgt taaaaagagg acatcctcct gccgactggg aaagcctcac
     2521 ccaagagaca gaagaaaacc attgatggtg ttcacaatgc ccgccgaacg cattggtgtt
     2581 gaccagcggg catcctttct cattccccga ccagaatcac ttcaacccca gcctttcgca
     2641 gtccttccag actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag
     2701 tatcaatgat cttatgtaaa ctggaacggt tgaacttact ggaatcggtg accacgatga
     2761 tccgttccgc aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgcgtgc
     2821 tgacgccacg ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat
     2881 tttgcagcga ttgctcagcc tgatcaccgt aaaaagattg cgactgacgg cgcaaatgcc
     2941 ccccggtcat cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac
     3001 cgttggtcat cgcaattacg tcggtgtgct tgcgcatcag acgagcaatc tcaaaagtgg
     3061 tggtcccgga gtcgaggatc acccgatgac caggctgaat caactcaacg gcagctttcg
     3121 caacgctgcg tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg
     3181 acggcgttgt gctatcgcag atcaacgcgc caccataggc acgcacggcg atcccctgct
     3241 tttccagaaa cgccagatcg ttgcggatcg tcacagtaga tacgccatac aatgccgaca
     3301 gatcgttaac ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct
     3361 cgctggtgcc tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt
     3421 tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca
     3481 agcccacctt acccattggc gcaagctact ctcgtttcag tggctttcat tatgtttctt
     3541 ttgtgaatca gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag
     3601 tatcaactga aacaaaacga aagattaatg tcgcagtaat ctgaactgga gaggaaagtg
     3661 aaacatctga cagaaatggt gagacagcac aaagcgggca aaacaaatgg gatttatgcc
     3721 gtttgttccg cacatccgct ggtgctggaa gctgcaatcc gctacgccag tgcaaaccaa
     3781 acgccgttac tgattgaagc tacctccaat caggtggacc agtttggcgg ttataccgga
     3841 atgacgcccg ccggttttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg
     3901 caggatgcgc tgattctggg tggcgaccat ctggggccaa accgctggca aaatctgcct
     3961 gccgctcagg caatggccaa tgccgatgat ttgattaaaa gctacgtggc ggcaggattc
     4021 aaaaaaattc accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaaccgat
     4081 ggcatcgtgg ccgaacgcgc cgctcgtttg gcgaaagtgg cggaagaaac ctgccgtgaa
     4141 cattttggcg aagccgatct ggagtatgtc attggtaccg aagtgccggt gcctggcggc
     4201 gcgcatgaaa ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg
     4261 gaagcccatc gtcacgcttt tgaaaagcag ggtctgaatg ccatctggcc acgcatcatt
     4321 gccctggtgg ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc
     4381 gccaaagcga ccgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg
     4441 cactctaccg attatcaaac gccgcaatcg ctgcgccagc tggtgattga ccactttgcc
     4501 attctgaaag ttggcccggc gctgaccttt gctctgcgtg aagctctgtt ctctctggca
     4561 gcgattgaag aagaactagt gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa
     4621 aacgtgatgc tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg
     4681 cgtcgtctgg cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc
     4741 cagattgatg acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgctg
     4801 ccgctgatca gtcagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag
     4861 ccaacgccac gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca
     4921 gcctgtgaag gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag
     4981 tcggattgat gaacgcttga ttcacggtca ggtcggcgtt cagtgggtag gatttgcggg
     5041 ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct
     5101 gatggaaatg gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagtcat
     5161 cgacaacatc catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacaccggc
     5221 cgatttcctg acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat
     5281 gcactacgcc aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggtgatat
     5341 cgcagcattt aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac
     5401 agagcctgct gtggacctct ttaaattact ttgagggatt catcatggaa atcagtctgt
     5461 tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggct
     5521 taacccatat gcaccgcccg gtggttctcg gcccgttggt cgggctggta cttggcgatc
     5581 tgcataccgg tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggccccgc
     5641 tggcgggcgc acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca
     5701 ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgttgcag
     5761 tacagatggg gattaccttc ctgttctcgg tgatgtccgg cgtgatgtcc cgctgcgacc
     5821 ggatggcgga gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg
     5881 cactcggcac tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg
     5941 caaaaaccat cattgatgtc ctgccgcaac gattaattga tggcctcggc gtcgcgggcg
     6001 gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca
     6061 tcccctactt catcctgggc tttgttgccg cagcctggct caagttaccg gtgctggcta
     6121 ttgctgccgc tgcgctggcg atggcgctga tcgacctgct gcgtaaatct cctgaaccga
     6181 ctcaacctgc ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg
     6241 aacgtcagcg aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat
     6301 cagagcattg gcgcagagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgctccatg
     6361 ctgttacaag cgtctttcaa ctacgaacgt atgcaggctt ccggctggtt gtacggtctg
     6421 ctgcctgctc tgaaaaagat ccacactaat aaacgggacc tggcgcgcgc catgaagggg
     6481 catatgggtt tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt
     6541 gcgatggagc gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt
     6601 gcgccgctcg gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc
     6661 gggataggcg caagcctcgc gctacaaggc tctattcttg gcgctgtcgt ctttattgtg
     6721 ctgttcaacg tggtgcacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg
     6781 ggcgtggcgg ctattccact cattaaagca aataccaaaa aagtcggcca tgcggcgtct
     6841 atcgttggga tgacggtgat cggcgcgctg gtggcaacct atgttcgttt aagcaccacg
     6901 ctggaaatca cggcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg
     6961 atgcctgcct tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc
     7021 tggagtccgc tgcgcctgat tgcagtgaca gtggttctcg gcatcgtcgg taaattctgc
     7081 catttccttt aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg
     7141 gatttgccag cggcatggaa aaagcaatga agcaaatcct cggcgagcaa tcgcagttta
     7201 tcgccatcga ttttccagaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg
     7261 ccatcgccca actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca
     7321 cgccgtttcg cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg
     7381 gcacaaattt gcaactatta ctggaaatgg tgctggaacg cgaagggtta agcggtgaag
     7441 agttccgtgt gcaggcgctg gagtgtggac accgtgggct aaccagtctg gtggacgaac
     7501 ttggtcgctg ccatgaggaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc
     7561 cagaaggctg ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg
     7621 tgtaatcgca gcaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct
     7681 ctgccccgct tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga
     7741 cgcgccggat gcgctcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg
     7801 gttaccgacc accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc
     7861 tctacgctgc cagcgcggtg ggcctggtgc gcaagtgctg gggagttatc tcgaaggacc
     7921 gtacttcacg ccgcagaata aaggcgcaca tccgccggag ctgtttcgcg agctggaaat
     7981 tgccgagctg gatcaattga ttgccgtttc tcagcagacc ttacgcgtgg tagcgctggc
     8041 accagaaaaa gaaggggcat tgcaggccat ccgccatctt aaacagcaaa acgtacgggt
     8101 gatgctgggg catagcgcgg cgacctggca acaaacccgt gccgcgtttg atgctggtgc
     8161 cgacggcctg gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat
     8221 ggttggcgcg ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca
     8281 tgtacatccg gcggcaatgt cgctctgttg ttgctgtgcg aaagagagga tcgtactgat
     8341 caccgacgcg atgcaggcag ccgggatgcc ggatggtcac tatacgttat gtggtgaaga
     8401 agtgcagatg cacggtggca ttgtccgtac cgcgttcggt gggctggcgg gcagtacgct
     8461 gtctgtcgat gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc
     8521 catccatatg gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc
     8581 gcttaaaccg ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca
     8641 aatctggatt cagggtcaat tagcttcgtt ttgatggttt gctcctttat taggccttca
     8701 cttcccccgt aaggcctttc tttttctttc tttttgatct gtgcagcggt gtcggatgcg
     8761 acgctaatgc gccttatccg acctacggtt ggtgaccgta aggccggata aagtatttac
     8821 gccgcatcca gcaatccctt ttacttccct tgtcttttct ttcaacgatc acagatttcg
     8881 ttttatttcc tttttctcca ttgaactttc agtttctttt ctatagattt taaccaacga
     8941 aagaaatcac caagtgaaat gaaacgaaag gcaagcgaaa gcgacaacgc cgacgtcaag
     9001 ttcatcagac taaggattga gttatgccag aaaattacac ccctgctgct gccgcaaccg
     9061 gtacatggac tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca
     9121 acattgacgc gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga
     9181 atctgcaaat catcctgacc ggagccggaa catcggcatt tatcggtgac atcatcgcgc
     9241 cgtggctcgc cagccatacc ggtaaaaact tcagcgccgt accaaccacc gatctggtca
     9301 ccaatccaat ggactacctg aacccagctc atccgctact gttgatctcc ttcggtcgct
     9361 ccggcaacag cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtaccagaat
     9421 gctatcacct gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca
     9481 gcgacaacgc gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga
     9541 ccagcagcat taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca
     9601 acagccaaac cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg
     9661 atttcagcga aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg
     9721 gtggcttaca gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acagcgggta
     9781 aactggcggc cttctatgat tccccgaccg gattccgtca tggcccgaaa tcgctggtcg
     9841 atgacgaaac gctggtagtg gtgtttgtct ccagtcaccc ttacacccgt cagtacgatc
     9901 ttgacctgct ggcagaactc cgccgtgaca accaggcaat gcgcgtgatc gccatcaccg
     9961 ctgaaagcaa cgacgttatc gccgctggcc cgcatatcat cctgccgccg tcccgtcact
    10021 ttatcgacgt tgaacaagca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc
    10081 agtcgctgca catgggcaat acgccggata ccccatcagc cagtggcacc gtaaaccgcg
    10141 tggtgcaagg cgtaactatt catccgtggc aggcataaga ggatcgcatt atgagcatta
    10201 tctccaccaa atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgccttta
    10261 acatccataa cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc
    10321 cggtgatcct cgccgggaca ccaggtacct ttaagcatat cgcgctggaa gagatctacg
    10381 ccctgtgtag cgcctattcc acgacctata acatgccgct ggcactgcat ctcgaccacc
    10441 acgaatcgct ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagc gcgatgatcg
    10501 acggcagcca cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gtcgacttct
    10561 gccactcccg ggattgcagc gtggaagcag aactgggccg cctcggcggt gttgaagatg
    10621 acatgagcgt tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg
    10681 tcgaactgac tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca
    10741 gcaaaacgcc gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc
    10801 ctctggtgct gcatggtgcc agcgatgtcc cggatgaatt tgtccgtcgc actattgaac
    10861 tcggtgtaac aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta
    10921 aagcctggtt tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa
    10981 tggatgcgat gaaagaagtg gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa
    11041 tttcagcata atcattaatt ttatacgttt tttcagaatg cactgcccga ttaatatcgg
    11101 gtagtacctt aatggcaaag gagataacca
//
LOCUS       DAPQZD010000157        10947 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPQZD010000157 DAPQZD010000000
VERSION     DAPQZD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.159"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            33..308
                     /gene="frmR"
                     /locus_tag="RO454_003989"
     CDS             33..308
                     /gene="frmR"
                     /locus_tag="RO454_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDY0935744.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            343..1452
                     /gene="frmA"
                     /locus_tag="RO454_003990"
     CDS             343..1452
                     /gene="frmA"
                     /locus_tag="RO454_003990"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDY0935745.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            1545..2378
                     /gene="fghA"
                     /locus_tag="RO454_003991"
     CDS             1545..2378
                     /gene="fghA"
                     /locus_tag="RO454_003991"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDY0935746.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(2503..3042)
                     /locus_tag="RO454_003992"
     CDS             complement(2503..3042)
                     /locus_tag="RO454_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDY0935747.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(3144..4355)
                     /gene="mhpT"
                     /locus_tag="RO454_003993"
     CDS             complement(3144..4355)
                     /gene="mhpT"
                     /locus_tag="RO454_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDY0935748.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(4531..5544)
                     /gene="dmpG"
                     /locus_tag="RO454_003994"
     CDS             complement(4531..5544)
                     /gene="dmpG"
                     /locus_tag="RO454_003994"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDY0935749.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGGDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(5541..6491)
                     /gene="mhpF"
                     /locus_tag="RO454_003995"
     CDS             complement(5541..6491)
                     /gene="mhpF"
                     /locus_tag="RO454_003995"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDY0935750.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(6488..7297)
                     /gene="mhpD"
                     /locus_tag="RO454_003996"
     CDS             complement(6488..7297)
                     /gene="mhpD"
                     /locus_tag="RO454_003996"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDY0935751.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(7307..8173)
                     /gene="mhpC"
                     /locus_tag="RO454_003997"
     CDS             complement(7307..8173)
                     /gene="mhpC"
                     /locus_tag="RO454_003997"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDY0935752.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(8191..9135)
                     /gene="mhpB"
                     /locus_tag="RO454_003998"
     CDS             complement(8191..9135)
                     /gene="mhpB"
                     /locus_tag="RO454_003998"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDY0935753.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(9137..10801)
                     /locus_tag="RO454_003999"
     CDS             complement(9137..10801)
                     /locus_tag="RO454_003999"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDY0935754.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
BASE COUNT         2561 a         3187 c         2793 g         2406 t
ORIGIN      
        1 tagtatattg cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa
       61 aggtccttac tcgagttcgt cgtattcggg ggcagattga tgctctggaa cggtcgctgg
      121 agggtgatgc cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta
      181 acggactgat ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact
      241 gctacagccg tgaagtcagc caatccgttg acgacactat tgaactggtt cgtgcctatc
      301 ttaaatagct gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc
      361 gttgcatttg ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa
      421 aaaggtgaag tgctgattaa agtcacccat accggtgttt gccataccga cgcatttacc
      481 ctctccggcg atgacccgga aggtgtcttc ccagtggttc tcggtcacga aggggccggg
      541 gttgtagttg aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatccca
      601 ctttacaccg cagagtgcgg cgagtgtgag ttttgtcgtt ctggcaaaac caacctgtgt
      661 gtcgcggttc gcgaaacgca gggcaaaggc ttgatgccag acggcaccac ccgtttttct
      721 tacaacgggc agccgcttta tcactacatg ggatgctcaa cattcagtga atacaccgtg
      781 gtagcggaag tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgcctg
      841 ctgggctgtg gcgtgaccac cggtattggc gcggtacaca acacagctaa agtccagcca
      901 ggtgattctg ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg
      961 cgtcaggcaa aagcgggacg gattatcgct atcgatacca acccgaagaa attcgaactg
     1021 gcgcgtcgct tcggtgccac cgactgcatt aacccgaatg actacgacaa accgattaaa
     1081 gatgtcctgc tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac
     1141 gtcaacgtga tgcgtgcagc gctggaaagc gcgcaccgcg gctggggtca gtcggtgatc
     1201 atcggggtag caggctccgg tcaggaaatc tccacccgtc cattccagtt ggtcaccggt
     1261 cgcgtatgga aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg
     1321 gttgaagatg cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc
     1381 ctggatgaaa ttaatgacgc cttcgacctg atgcatgaag gtaaatccat tcgaaccgta
     1441 attcgttact gatttcccgc tggtttaccc cgtccagttc agatggggtt cttaatactc
     1501 tccccgggca gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa
     1561 aacatgccag ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat
     1621 gtgaaatgaa tgtcggcgtc tatctccccc caaaagccga gaatgaaaaa ttgccggtgt
     1681 tgtactggct ttcaggcctg acctgcaacg agcaaaattt cattactaaa tcggggatgc
     1741 agcgttacgc ggctgagcac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca
     1801 gtcatgtcgc agatgctgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg
     1861 cgacgcaagc gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc
     1921 cgaatttagt gatgcatcat tttccggcaa cggccagaaa gtcaatcgca ggtcattcta
     1981 tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct
     2041 cggcgttttc gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg
     2101 catatcttgg tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac
     2161 aaggtcaacg cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcgg
     2221 aacaactgcg gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa
     2281 tccgttatca cgagggttat gatcacagct attattttgt ctccagtttt attggcgagc
     2341 atattgccta ccacgccaat aaactgaata tgcgttgagc atccgcgcac gcatgccgga
     2401 tgcggcgtaa accttatccg gcccacattt cgctcgtaaa ccgtaggcct gataagacgc
     2461 gaaaagcgtc gcatcaggca tctgcacacg atttatcaat gtttaccaca tcaaatcatc
     2521 aggcactttg aagtcggcat acggatcgtc ttcatcctgc tcttcggcgc tgagcgcgct
     2581 gtgcaacaca atactgctgg catcacgctg agcaatttta tcggcaacgc tggcgggaat
     2641 aatggcgtat tcaccttcgc tattgttatc gaccaacaag cgggcaatcg ccagacgacc
     2701 attaatcaac tgcgtttgcg tgagcttatc gacaaaaatc ttcttaatca gattgccgtc
     2761 agtgaagtta aaaccaatat cgccattggc aatggtaatt cggttcattt caatgagctg
     2821 cttcacctgg gctttatatt ctttcgccaa cgccgcttgt ttttgttgct cgctaagctg
     2881 tttatcacgc tcaagctgtg cctttttatt ttcttctacc gccgcccgtg cttcacgcgc
     2941 ctgaacacgc gattttttcg ccgttctctc gaccttcgcc gcttttttgc tggtgactag
     3001 tcccgctttg agcaactgct cttgtaaggt aagttttgcc atctttgttt ccaaacccgc
     3061 tgaataattt ttagcgagta tacctgtaaa cgccgtaggt gtattggggg caagtccctt
     3121 tcgcccctgc ttaataaggc gcgtcaggca tcggcacacg gctgcattct tgatctccga
     3181 ctcatcaaaa taaacaccgc caacccagca acaagaatac ccggtgcaga agcggccatt
     3241 acgccgaccg tgccagtgcc taatgccagc attttcccgg ccagtaacgg accgctcata
     3301 gcccccagac gccctaccgc cacggctgtt cccacacctg ttgcgcggat ctgcgaactg
     3361 taaaacaacg gtgccagagc atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg
     3421 acaaatcccg ccagcaacat actgttaaat gacgacaccg ttcccagcgc cagcagcgaa
     3481 gctaacatgc cgctataaat cagtagcgac atggttactg gacgcagttt atccatcaat
     3541 gcgcccaaca ttaacgtccc gcttgccgcc cccatttgca gtgcaaacat cacccctgcc
     3601 gcctgcgatg gctggaatcc ttgctccacc aaaagcagcg gcagccagtt gatcaacatg
     3661 tagaccacca gcagagtgaa aaaataacac aaccacagca gcagcgtcgc ggttgccgtt
     3721 tctggcgcaa ataacgcacg cagtggtggc gcagcctgtt tttcgccagc gaaaaccgtc
     3781 gactccggca gccagcgcat taatagcggc accagaatca acggcaccac accacctacc
     3841 caaaacaccg tttgccatgc taagtttgcc cccgcgaaac ccagcgtcgc cgccagcgcc
     3901 gcgccaatgg gaacaccgca atacatcagg ctcactgccg tcccacgaaa acgtggtccc
     3961 gcggcttcag acgtcagggc gataagattc ggcaacgccg cccccagccc gacaccggtc
     4021 atcagccgcg caaagaccag tgaggggaaa tcccatgcaa tcgccgttgc cagcgagaac
     4081 aaaccaaaca gcgcaactga gccaatcaaa atacgcttac gcccataacg gtctgccagc
     4141 atcccgccaa ccagcgcgcc gggtagcaaa ccgagtattc cggcgctaaa tatccagccc
     4201 atttgcattt tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc
     4261 tgaagatcca gcccttccat cagagcgacc aaaaaacaaa gcccgatggt cagcatcagg
     4321 cgggatgaag atgatgaagg ggtacgagtc gacataagat gaacctcatt gcgagtgcag
     4381 gtgaatcgcg actgatacat tgccagccgc gtaatcagaa ctatttaatg actgcctacg
     4441 ggcgaaatgt aggccggata aggcgttcac gccgcatccg gcagtcgtgc tgggatgggc
     4501 atacaaaatg cacgttctcg gcagtcaata ttatttgttg ttgcgcagat ccagcgccac
     4561 gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat
     4621 atccaccgcg cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctgga
     4681 gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gtcgatcctg
     4741 caacggacgc accaggtcgt cagcggcatc cattaacgca tagagatcgc ccccatgctg
     4801 ccaacccagt ttatccgcgg cggcaataaa tacttccagc ggcgcgttac ctgcgcccgc
     4861 gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga
     4921 attcgccacg ccaagactca ggttatggtg agcgtgcatg ccggtttgcg tttctggttt
     4981 cagcactgct ttcagggcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc
     5041 agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg cctgctttgc
     5101 gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag
     5161 ctcgcgggca tactgaatat gctgggcgga aacatcagcc tcggtacagt gcgttgccac
     5221 acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc
     5281 tggcagcaac aacgtcgcga ttttggcgtg cttcaccaca tccgccgccg cttcaatcca
     5341 ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccctgca aaccgtcgcc
     5401 gtgggccact tcaatcgaat ccacgcgggc atcatccagt gctttggcaa tctggcggac
     5461 attttccagc gaatactgat gacgaatggc gtgcataccg tcacgcaacg tgacgtccga
     5521 aatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc
     5581 attttttccg ctgtcgccag cgcactggaa gtcataatgt cgaggttgcc cgcataggca
     5641 ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ccgttttcag cccggagaac
     5701 tgccccacgc ccggtaaatt gaccggttta tcctgcggga tgacttcaaa ctgcacgcgc
     5761 tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag
     5821 gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc
     5881 aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccacccacc
     5941 acttcaatgg cgcgcgaagt ggtttccgta aattcatcga tgttggcacg cgtgccgggt
     6001 ccggcagatt tactggcaat agaagcgata atttcggcgt aatgaacacg cgccacgcgt
     6061 gaaactgccg ctaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc
     6121 agttgagcga cgttctcctc gaggttaacc accggcacgc agtaagggcc gatggcagca
     6181 ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gcaaagcggc atcgtttttc
     6241 acatgcgcgc ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg
     6301 ttcatcagcc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc
     6361 agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg ctgaccgtga
     6421 cgcaaaattt taatcatcag atcggtgcca atgttgcctg aaccgataat ggcgacttta
     6481 cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac
     6541 ctatgccttc gatatgggct tcaaaacgat cgcccgcatt caccgccacc atcggaccta
     6601 atgccccggt aagaatgata tctccggcgc gcagcggttc acccagactg gccattttgc
     6661 gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg
     6721 aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg
     6781 ggcgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat
     6841 cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacctccagc gccggaagta
     6901 cccattcaat ggcgttatac aattcgtcga aggtgatatc ggttgcgggt agatcgcggt
     6961 tcaacaccag tgcaatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatgg
     7021 tttcattatc gccataacac atgtcggcaa ataatgtccc gaaatccggt tgatcaacgc
     7081 ccagttgttg ctgcactttc ggatgcgtca gccccacttt gcgccccacc acgcgacgcc
     7141 cctgcgcaac gtcatgttgc acattgatgt gctgaatggc gtaggccgct tcagcgttat
     7201 cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta
     7261 aatccgccgc cagttgctca agagtatgct tcgtcatgac cgttccttaa gggcgtgcga
     7321 ggaaattcag caccagttga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac
     7381 agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat
     7441 ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg
     7501 ccagacgtgg gccaaaatcc gggaactgtt tcggattggc ttccaggctc ttaacgaagt
     7561 tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg
     7621 tcaaatcgct ggtatcaaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg
     7681 gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac
     7741 tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa
     7801 gagtgaacgc cacagaactg tggccaccca tcgagttgcc cagcaggtgg attttggcga
     7861 tatccagttg atccaccacg cttttcagga ttcgcgcatt aagatccgat cgcgaaccac
     7921 tattaacgat cgaatcgctc ttaccccaac ccggacaatc cagcaggatc acccgatagc
     7981 ccgcctctac cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc
     8041 cggaaccatg cagcaggaca acggtttcgt cgccttgtcc gcagtcatta aaatggatgc
     8101 gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg
     8161 gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc
     8221 aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc
     8281 aaacgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc
     8341 ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc
     8401 cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagcg ttctctgatc
     8461 ctcaacaaac ttctcagcgg cgctaatcac ccgttgctga cgcaattcgc gctcactggc
     8521 gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag
     8581 ttcgggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt
     8641 gagagtactg gtgaaacgtc caatggcttc ccccaacatg cgggtacgct ggaaaccggg
     8701 cagcggcgtg gcgacaccgt tgatgaacac cggcagtact ggcaccttat ccagcccacc
     8761 gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc
     8821 aagatcgatc ccgctcttca tgacggcatg cgcacaggcc tccgccagct ccacaggcac
     8881 gggcagctct cctgccgcac tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca
     8941 gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac
     9001 cagttcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc
     9061 atcgagtacc tcttgtgccg ggtcgacata ccccaccagc ggcgagtggg aaagacagtg
     9121 aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca
     9181 gcgtcatcac cgacgccagt ttattcaggg tcttgcccag ggtttgcgga atggcggtgg
     9241 cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc
     9301 tacgcaggcg accttgcgta tcccccacgc gtagtacgcc gtcgtggtta tcctgtgcgg
     9361 tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct
     9421 ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt
     9481 tcgcgccgat cgcgttatcg agtagcgtca cgtcgccgtt ttccagcgtg actttcggct
     9541 gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc
     9601 cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca
     9661 gataattcag cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag
     9721 ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt
     9781 cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt
     9841 tccatgcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg
     9901 gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacgaaaa cgttgcgcca
     9961 gtcgcgcgtt gtgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca
    10021 gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgttct tcggtttctc
    10081 ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg
    10141 ggcgcaccgg atcacaacac aaatagatat gcggcgtact taacggatcg ttggcgatat
    10201 ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac
    10261 ggacaaaact tgccccacca tcacaggcca ccagccactg ggctttgacc gtttcccgct
    10321 gcccttcttc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca
    10381 gctcgcggga aaacaggcag cgcacattcg gaaaacgcga caatccttcc agcatcaccg
    10441 catcgacctg aggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg
    10501 gctgaatatc agcaaaacag cgaccttttg gggtgagaaa acgcatcgcg tgccacggcg
    10561 tagtgtgcgg cagaacatta tcgaccaggc caaccgactg catggtgcgc agcgcctcgt
    10621 catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca
    10681 cgtcaatgcc catctggccg agatagttcg ccatcatcag cccgaccgga ccggcaccag
    10741 cgatcgccac ctgaacgcta tggttaacag caggctggat gtcagggtgt tgtattgcca
    10801 tttcagtacc tcacgactcg gacaaaatgc cgttgcgcgc acagtacagc gcaacttatt
    10861 ttgttaaaaa catgtaaatg attttttatt gtgcgctcag tataggaagg gtgttttcgg
    10921 ctacaatcaa aacatgcccg aatgtgc
//
LOCUS       DAPQZD010000158        10923 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPQZD010000158 DAPQZD010000000
VERSION     DAPQZD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.160"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            259..762
                     /gene="dps"
                     /locus_tag="RO454_004000"
                     /gene_synonym="pexB"
     CDS             259..762
                     /gene="dps"
                     /locus_tag="RO454_004000"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDY0935755.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1166..1912
                     /gene="glnH"
                     /locus_tag="RO454_004001"
     CDS             1166..1912
                     /gene="glnH"
                     /locus_tag="RO454_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDY0935756.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2051..2710
                     /gene="glnP"
                     /locus_tag="RO454_004002"
     CDS             2051..2710
                     /gene="glnP"
                     /locus_tag="RO454_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDY0935757.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2707..3429
                     /gene="glnQ"
                     /locus_tag="RO454_004003"
     CDS             2707..3429
                     /gene="glnQ"
                     /locus_tag="RO454_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDY0935758.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3546..5771
                     /gene="ybiO"
                     /locus_tag="RO454_004004"
     CDS             3546..5771
                     /gene="ybiO"
                     /locus_tag="RO454_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDY0935759.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPRIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5768..6694)
                     /gene="rlmF"
                     /locus_tag="RO454_004005"
     CDS             complement(5768..6694)
                     /gene="rlmF"
                     /locus_tag="RO454_004005"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDY0935760.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6970..7230
                     /locus_tag="RO454_004006"
     CDS             6970..7230
                     /locus_tag="RO454_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0935761.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7495..9777
                     /locus_tag="RO454_004007"
     CDS             7495..9777
                     /locus_tag="RO454_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDY0935762.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9819..10496
                     /gene="ybiX"
                     /locus_tag="RO454_004008"
     CDS             9819..10496
                     /gene="ybiX"
                     /locus_tag="RO454_004008"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDY0935763.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10570..10836
                     /gene="ybiI"
                     /locus_tag="RO454_004009"
     CDS             10570..10836
                     /gene="ybiI"
                     /locus_tag="RO454_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDY0935764.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT         2669 a         2826 c         2757 g         2671 t
ORIGIN      
        1 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
       61 cgaaaattcc tggcgagcaa ataaataaga attgttctta ttaatatatc taactcattg
      121 atttttttat tgtttttgtt tttcacgctt gttaccacta ttagtgtgat aggaacagcc
      181 agaatagcgg aacacatagc tggtgctata cttagtctcg ttaattactg ggacataaca
      241 tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatctg
      301 ctttataccc gcaacgatgt ctctgacagc gagaaaaaag caacagtaga gttgctgaat
      361 cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg
      421 cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac tgcactgatc
      481 gatcatctgg ataccatggc agaacgtgca gtgcagctgg gcggtgtagc tctggggacc
      541 actcaagtta tcaacagcaa aactccgctg aaaagttacc cgctggacat ccacaacgtt
      601 caggatcacc tgaaagagct ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa
      661 gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac
      721 ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg ctgatggtgc
      781 agaactttag tacacgataa aagcggcttc ctgacaggag gccgttttgt tttgtagccc
      841 gcctcaacgc acttatttag tgcatccatc tgctatcccc agctggttaa gtaaattttt
      901 tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag gttttacgct
      961 aaataatcac tgtgttgagt gcacaatttt agcgcaccag tttggtgccc cagaatggtg
     1021 catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc
     1081 aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca ggggatcgtc
     1141 ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact
     1201 gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga
     1261 taccgccttc gttccgtttg aatttaaaca gggcgataaa tacgtgggct ttgacgttga
     1321 tctgtgggct gccatcgcta aagaactgaa gctggattac gaactgaagc cgatggattt
     1381 cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac
     1441 catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct
     1501 gttagtgatg gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa
     1561 agtggttgct gtgaagagcg gcactggctc cgttgattac gcgaaagcaa acatcaaaac
     1621 taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg
     1681 cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa
     1741 cggtcagttc aaagcggtag gtgactctct ggaagcacag caatacggta ttgcgttccc
     1801 gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa
     1861 cggaacttac aacgaaatct acaaaaaatg gttcggtact gaaccgaaat aataacgctg
     1921 cgcctgtaaa gcgcactggc agttccctct ccccgccggg gagaggatta gggtgaaggg
     1981 cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca
     2041 ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga
     2101 aggtgccaaa atgaccctgt ggatttcggt cctcggtctg gctggcggtc tggtgatcgg
     2161 attgttggca ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt
     2221 ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc
     2281 cctgccgatg gcgtttaacg atttacgcat cgacccgttt actgcggcgg tggtcaccat
     2341 catgatcaac tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctattca
     2401 caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta
     2461 cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat
     2521 cagcattaaa gacacttcgc tgtttattgt tatcggcgtg gcggaactga cccgtcaggg
     2581 gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt
     2641 ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa
     2701 aatcctgtga ttgaatttaa aaacgtctcc aagcattttg gtccaaccca ggtgctgcac
     2761 aatatcgatt tgaacattgc tcagggcgaa gtcgtggtga ttatcgggcc gtccggttcc
     2821 ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg
     2881 attgtcgatg gcctgaaggt taacgatccg aaagttgatg agcgcctgat tcgccaggaa
     2941 gcgggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct ggaaaacgtc
     3001 atgtttggcc cgctacgcgt gcgtggcgcg aataaagaag aggcggaaaa actggcgcgt
     3061 gagctgctgg cgaaagtcgg tctggcagag cgtgcacatc actacccttc cgaactttct
     3121 ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg
     3181 ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt
     3241 atgcaggatc tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt
     3301 gccgagaaag tagcttcgcg cctgatcttt atcgacaaag gccggattgc agaagatggc
     3361 gatccgcagg tgttgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac
     3421 gtctcttaac ttctctgcct ccctttcccc cgaaagggag gcacaccaga ttcctctcat
     3481 ttaaaattgc ctctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag
     3541 gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg
     3601 tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg
     3661 aaccggatat cgaacaaaaa aaagcggcct atggcgcact ggcggatgtg ctggataatg
     3721 acacctcgcg taaagagttg atcgaccagt tgcgcaccgt cgccgcgacg ccccctgcgg
     3781 aaccggtacc gaggatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg
     3841 tcaccgaagt cagccgccat tatggtgaag ccctttccgc ccgcttcggg caactttatc
     3901 gcaatatcac cggctccccg cataagccgt ttaatccaca aaccttcagc aatgcgctga
     3961 cccacttttc aatgttagcg gtattagtgt ttggttttta ctggctgatt cgtctgtgcg
     4021 cactgccgct gtattgcaaa atgggccagt gggcgcggca aaaaaatcgt gagcgcagta
     4081 actggttgca gcttccggcg atgatcatcg gggcgtttat tatcgacctg ctgttactgg
     4141 cactgacatt atttgtcggc caggtattaa gcgacaacct gaatgcgggc agtcgcacca
     4201 tcgctttcca acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaaaccg
     4261 tactacgcct gattttttgc ccaaacgtag ctgaactgcg cccgttcacg attcatgacg
     4321 agaccgcccg ttactggagt cgtcgcctga gctggttaag cagcctgatt ggctatggtc
     4381 tgattgtggc tgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga
     4441 acgtcatcat tatgctgtgc atgaccgtct gggcgttgta cctgattttt cgtaataaaa
     4501 aagagatcac ccagcatttg ctcaactttg cggagcattc gctggccttt ttcagcctgt
     4561 ttatccgcgc ctttgcactg gtgtggcact ggctggcaag cgcctatttt atcgtgctgt
     4621 ttttcttttc gttgttcgat ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc
     4681 gcagcctggc gattattggt atcgcagcat ttgtttccgg tatgttttcc cgctggctgg
     4741 cgaaaaccat caccctctcg ccacatactc agcgaaatta tccggagctg caaaaacggc
     4801 tgaatggatg gctttcggcg gcactgaaaa cagcgcgtat tctgacagtc tgcgtggcgg
     4861 taatgctgct gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggtg
     4921 cggggcagaa aaccgtagat atcctgatcc gtatcgcact cattcttttc ttctcggcgg
     4981 ttggctggac ggtgctcgcc agtttgatag aaaaccggct ggcttcggat attcatggcc
     5041 gcccgctacc cagcgcccgt acgcgtaccc tgctaacgct gttccgtaac gcgctggcgg
     5101 tgattatcag taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc
     5161 cattgctggc aggtgccggt gcattgggac tggctatctc ttttggttcg caaacgctgg
     5221 tgaaagatat tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt
     5281 tggtgactat cgggccgttg accggcactg tggaacggat gtcaattcgc tccgtgggcg
     5341 tgcgccagga taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta
     5401 acttcgtccg cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg
     5461 ctgataaagc caatcaggca ctgaaagatg cggtagcaga attgatggaa aacgaagaaa
     5521 ttcgcgggct ggttattggt gaaccgaatt ttgccgggat tgtcggctta agcaataccg
     5581 cgtttacact gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtacgctttg
     5641 ccctcgacag ccaggtgaaa aaacatttcg acctggcggg tgttcgcgcg ccggtgcaga
     5701 cttatcaggt gctgccagct ccgggcgcgg ccccggctga accgttgccg ccgggggagc
     5761 caacgcttta acgctggcga ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc
     5821 aggctataaa gcggctctgc ttttgcccct gggccatctc tttttttacc accttcaccg
     5881 cacctacgtc cgtcaaagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg
     5941 aggtaaacca catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa
     6001 aagcgacctc accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat
     6061 cgtctttgtt cagccccagg ttacggcgtt tacgttcact acctgcccgt gccgcagcgg
     6121 cggaatcgtg gaatggcggg ttacacaagg tcgcatcgta ttgctcgttt ttatggatga
     6181 tgccgttaaa aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc
     6241 ccggattagc actgatgatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac
     6301 cggtaaaacg ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa
     6361 cgccgatatc cagaatgctg gcattagctg gaattgttcc gcttgcttcg gccagtaaat
     6421 cggcaaggtg atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtccg
     6481 ggatatccca gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca
     6541 ccgccagcgg attggcaaag tctacgcttt gctccccggc gggtgtaagc gtgaggaatt
     6601 gcctgagttc aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt
     6661 tgcgcggatg caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta
     6721 agatacccgc ataacgcgac agggtaaata tctggctgcg tttatcattt ctaaaaacct
     6781 tcacatggct atggtttatg gcaattcacc ataaaaaaca tcatcatgca tgtcattgat
     6841 ttgtcttatt ttgtaaaaac acatcgttca aaaaataagc gcatctctgt caattttttg
     6901 cgtcattttc atcgcgcagg cgacctacac ttatctggca ctacccgttg tatttatgag
     6961 gatgtcatta tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct
     7021 ctcacggctt atgccgcgca accgatgagt aacctggaca gtggtcaact acggcccgcc
     7081 ggtaccgttt cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa
     7141 aaagcgcgcg aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag
     7201 atgttcggta ctgcaatcat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg
     7261 atgcagtttt cgtccaattt gtaagcaaaa aacatcaaat atgaacgcaa tgtaaataaa
     7321 tgtatttctt tttcgcgcaa tgggtgatag aaaatcgcgc caagtgataa tgcttatcaa
     7381 tattattatc acttgcacgt acgctatcac gggattaaca gtggcatcgc atccgcagag
     7441 atgctttctc gtgacagtga aaatttcacc atataagaaa aagtcacctg caaaatggaa
     7501 aacaatcgca atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt
     7561 attggtatta cgcctgtggc acaggcactc gctgccgaag ggcaagctaa cgcggatgac
     7621 acgctggttg tcgaagcatc aacgccttcg ctttatgcgc cacaaaaatc tgccgatccg
     7681 aaattctcgc gtccggtagc ggatactacc cgcacgatga cagtgatttc tgaacaagtg
     7741 attaaagatc agggtgcaac caaccttacc gacgcgctca aaaacgtccc cggcgtgggt
     7801 gcgttttttg cgggtgagaa cggtaactcc accactggcg acgccattta tatgcgcggt
     7861 gccgatacct ctaacagtat ttatatcgat ggcattcgcg atatcggcag cgtctcgcgc
     7921 gacaccttca ataccgagca ggtcgaagta attaaagggc cgtccggcac cgactacggg
     7981 cgcagcgcac cgacaggctc gatcaatatg atcagcaaac agccgcgcaa tgattccggc
     8041 attgacgcct ccgccagtat tggcagcgcc tggttccgcc gcggcacgct ggacgtcaat
     8101 caggtcattg gtgataccac tgcggtgcgc ctgaatgtaa tgggcgaaaa aacgcacgat
     8161 gccggacgcg acaaagtcaa aaatgagcgt tacggcgtcg ccccttctgt cgcttttggc
     8221 cttggtacag cgaatcgttt gtatcttaat tatctgcatg tcacccagca caacacgcca
     8281 gacggcggca ttccgaccat cggtttaccg ggtttttctg ccccatctgc gggaacagcg
     8341 gccctgaatc attccggaaa agttgatacg cataactttt acggcacgga ttccgattac
     8401 gacgattcga ccaccgacac cgctaccatg cgttttgagc acgacatcaa cgataacacc
     8461 accattcgca acactacgcg ttggtcgcgc gtaaagcagg attacctgat gacggcgatt
     8521 atgggtggag cgtcgaatat tacccagccc accagcgatg tgaatagctg gacgtggtca
     8581 cgcacggcga ataccaaaga tgtgagtaac aaaattctca ccaaccagac caacctgacc
     8641 tcgacgttct ataccggcgc tatcggtcat gatgtcagta ccggcgtgga atttacccgc
     8701 gaaacacaga ctaactatgg cgttaatccg gtgacgttac cgacggtaaa tatttatcat
     8761 cctgacagca gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc caatggtcag
     8821 acggatacct tcgcaattta tgccttcgat acgctgcaaa tcacccgtga ttttgagctg
     8881 aacggtggga tccgtctgga taattatcat actgaatatg acagtgccac cgcctgcggc
     8941 ggcagcgggc gcggtgccat cacctgccct gctggcgtgg caaaaggttc tccggtaacc
     9001 accgtcgaca ccgccaagtc gggcaatctg gtgaactgga aagccggggc gctgtatcac
     9061 ctgacggaaa acggcaatgt ttatattaac tatgccgttt cccagcagcc gccgggcggc
     9121 aacaacttcg cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac cgattttaaa
     9181 ccgcaaaaag ccaacaccag cgagattggc accaaatggc aggttctgga taaacgcctg
     9241 ctacttaccg ccgcgctgtt ccgtactgat attgaaaatg aagttgagca aaatgatgat
     9301 gggacttatt cgcaatatgg taagaaacgt gtcgaaggct atgagatatc cgtggccggg
     9361 aatatcaccc ccgcgtggca gatgattggc ggctataccc agcaaaaagc aactatcaaa
     9421 aacggcaaag atgttgccca ggatggttcc tcatcgctgc cgtatacccc ggaacacgcc
     9481 ttcaccttat ggagccagta tcaggcaacc gatgatattt ctgttggtgc gggcgcacgg
     9541 tatatcggca gtatgcataa aggttcagac ggcgcggtgg gaacgccagc atttaccgaa
     9601 ggttattggg tcgccgacgc caaactgggg tatcgggtta atcgcaatct cgacttccag
     9661 ctaaacgtct acaacctgtt tgataccgat tacgtcgcct caatcaataa gagcggctac
     9721 cgttatcacc cgggcgagcc aagaaccttc ttgctcacag ccaatatgca tttctgattc
     9781 agatgtgggg cgcaggcccc actttttgga gaacttgtat gatgtaccac attcccggcg
     9841 tgttatcgcc acaggacgtc gcccgttttc gcgaacacct ggaacaagcc gaatgggtgg
     9901 atggacgcgt caccaccggc gcacaaggtg cgcaagttaa gaacaatcaa caagtagaca
     9961 cccgcagcgc cctgtacgcc gccctgcaaa atgaggtgct gaacgcggtt aaccaacatg
    10021 ctttgttctt tgccgcggcc ttgccgcgta ccctttccac gccgctgttt aatcgctatc
    10081 agaacaatga aacctatggt tttcatgtgg atggcgcagt acgcagccat ccacaaaacg
    10141 gctggatgcg cactgacctt tctgccacgc tgtttttaag cgatccagaa agctacgacg
    10201 gcggcgaact ggtcgttaat gacaccttcg ggcaacatcg ggtaaaactc ccggcaggcg
    10261 atctggtgtt gtatccctcc agcagcctgc attgcgtgac acccgtaacc cgcggcgtgc
    10321 gagtggcatc atttatgtgg atccagtcga tgatccgcga tgataaaaag cgcgccatgc
    10381 tgtttgaact ggacaacaat attcagtcgc tgaaaagcca ctacggcgaa agtgaagaga
    10441 tcctgtcgct gctcaatctt tatcataatc tgctgcggga atggtcggag atctgacgct
    10501 tgaactgccc gagttcgtcc ctatattagc tacacttaac tgtacaagca ttgatatggg
    10561 gaggacgata tggcatccgg ttgggctaat gatgacgccg tcaacgaaca gatcaacagt
    10621 acaattgaag atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga aagcctgtat
    10681 gaatgtgaag agtgcggtgc ccctatcccg caggcccgtc gggaagccat tcctggcgtg
    10741 cgcttatgca ttcattgtca gcaggagaaa gatttacaaa aaccagctta tacaggatat
    10801 aatcgcagag gttcgaaaga cagccagtta cgttaactac aactggcggg atgtatagcg
    10861 aatataattt gtattttgcg tggcattccg tgcctttaac gctaccgtga ctgccacgcg
    10921 gta
//
LOCUS       DAPQZD010000159        10902 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPQZD010000159 DAPQZD010000000
VERSION     DAPQZD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.161"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(21..317)
                     /locus_tag="RO454_004010"
     CDS             complement(21..317)
                     /locus_tag="RO454_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDY0935765.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            541..1257
                     /gene="tonB"
                     /locus_tag="RO454_004011"
     CDS             541..1257
                     /gene="tonB"
                     /locus_tag="RO454_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDY0935766.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(1297..1695)
                     /gene="yciA"
                     /locus_tag="RO454_004012"
     CDS             complement(1297..1695)
                     /gene="yciA"
                     /locus_tag="RO454_004012"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDY0935767.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1800..2339)
                     /locus_tag="RO454_004013"
     CDS             complement(1800..2339)
                     /locus_tag="RO454_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDY0935768.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2369..3112)
                     /locus_tag="RO454_004014"
     CDS             complement(2369..3112)
                     /locus_tag="RO454_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDY0935769.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3140..3229)
                     /locus_tag="RO454_004015"
                     /pseudo
     CDS             complement(3140..3229)
                     /locus_tag="RO454_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3468..4106
                     /gene="ompW"
                     /locus_tag="RO454_004016"
     CDS             3468..4106
                     /gene="ompW"
                     /locus_tag="RO454_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDY0935770.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            complement(4152..5282)
                     /locus_tag="RO454_004017"
     CDS             complement(4152..5282)
                     /locus_tag="RO454_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDY0935771.1"
                     /translation="MARPRKYKTNVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(5260..5508)
                     /locus_tag="RO454_004018"
     CDS             complement(5260..5508)
                     /locus_tag="RO454_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDY0935772.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(5573..8044)
                     /locus_tag="RO454_004019"
     CDS             complement(5573..8044)
                     /locus_tag="RO454_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDY0935773.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQ
                     PDLVKVTADEVNKIMQAASISQPDANKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     HQNQSETEQYDQKAEQNSQNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCG
                     AVMGDATYQETFNEESQDEAREKDPEEIENAGLPNKECTEGDQHANDNNEIGETANPL
                     IKVNSHRKSTSTSRLWHHLMIDLETMGKNPDAPIISIDAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFENTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            complement(8137..8328)
                     /locus_tag="RO454_004020"
     CDS             complement(8137..8328)
                     /locus_tag="RO454_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDY0935774.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(8325..8513)
                     /locus_tag="RO454_004021"
     CDS             complement(8325..8513)
                     /locus_tag="RO454_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDY0935775.1"
                     /translation="METLLPNVNTSEGCFEIGVNISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            complement(9079..9297)
                     /locus_tag="RO454_004022"
     CDS             complement(9079..9297)
                     /locus_tag="RO454_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935776.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARTKQAE
                     NLSALEQDITRLEKAAALSIARMVTYPR"
     gene            complement(9457..9612)
                     /locus_tag="RO454_004023"
     CDS             complement(9457..9612)
                     /locus_tag="RO454_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDY0935777.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(9885..10601)
                     /locus_tag="RO454_004024"
     CDS             complement(9885..10601)
                     /locus_tag="RO454_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0935778.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            10651..10866
                     /locus_tag="RO454_004025"
     CDS             10651..10866
                     /locus_tag="RO454_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0935779.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT         2728 a         2624 c         2526 g         3024 t
ORIGIN      
        1 cttaaatatt gaatttctat tcagaacact ttcttaaatg gtttcactga aacgtgttca
       61 tagactcctg ccgctacgta cgggtcagca tcggcccagg cctgagctgc ttccagcgac
      121 tcaaattcag caataacggt tgagccagta aatcccgcag cccctggatc gttactgtct
      181 accgctggca ttggaccggc ggtcaacaaa cgaccttcat catgcagtaa ctgtaaacgt
      241 gctagatgtg ccggacgaac ggaaaggcgt ttttcgaggc tatcagcttt gtcttgagcg
      301 taaataacat acaacacagg cacaactcct tgttcgggaa agttgtaaag tacgttattt
      361 gaaagggcga agatctgcaa cggaaagatg atgtctttgt taagaccatg cataaagtaa
      421 ggtcaattac gccaaaaatg acattttcac tgatcctgat cgtcttgcct tattgaatat
      481 gattgctatt tgcatttaaa atcggggcct ggtttttcta ctgaaatgat tatgacttca
      541 atgacccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
      601 ggtgctgttg tggcgggttt gctctatacc tcggtacatc aggttattga actacctgcg
      661 cctgcgcagc cgatttctgt cacgatggtt gcgcctgctg atcttgaacc gccacaagcc
      721 gttcaaccac caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccctgaa
      781 ccgccaaaag aagctccggt ggtcattgaa aagccgaagc cgaaaccaaa gccaaaaccg
      841 aagccggtga aaaaggtgga gcagccaaaa cgcgatgtca aacccgtaga gtcgcgtccg
      901 gcatcaccgt ttgaaaatac ggcacccgcg cgcccgacat caagtacggc aacggctgca
      961 accagcaagc cggttaccag tgtggcttca ggaccacgcg cattaagccg taatcagccg
     1021 cagtatccgg cacgagcaca ggcattgcgc attgaagggc aggttaaagt taaatttgat
     1081 gtcacgccag atggtcgcgt ggataacgta caaatcctct cagccaagcc tgcgaacatg
     1141 tttgagcgtg aagtgaaaaa tgcgatgcgc agatggcgtt atgagccggg taagccaggc
     1201 agtgggattg tggtgaatat cctgtttaaa attaacggca ctaccgaaat tcagtaagca
     1261 taaagtcaaa agcctccgac cggaggcttt tgattattac tcaacgggta aggcgcgagg
     1321 ttttccttcg ggatcaaccg cgacatactt aaataatgct tctgttgctt tatagcgttg
     1381 cccgattggt tctgacgcca cttttttcac ccacacttca atattaatgc tgactgatgt
     1441 cgtccctttc tggacacagc gtgcatagca gcacaccaca tcgccgaccg caaccggccg
     1501 taagaaagtc attccttcaa cccgcacggt cactacacga ccgtgggcaa tttctttcgc
     1561 cagaatagcg ccgccaatat ccatttgtga cattaaccaa ccaccaaaga tgtcaccatt
     1621 ggcattggta tcggcgggca tggctaaagt acgtaaaaca agatcgccct gagggacgtt
     1681 atgtgctgta gacatggtaa aaccgactta aaaggaaaat cacaggcgcg atgctactat
     1741 gattttcctg aggagaacag aggagataca gcgccagccc cgaaggactg gcagtctggt
     1801 taggatttat cttcctgcgg catgtggcgg tagatataga taccgcttaa caatgtgaag
     1861 attaaggtaa gggcggtcag gccaaagact ttaaagttga cccaaatatt ttgcggcagc
     1921 cagaatgcga tgtagatgtt tgccaggccg caaaggataa agaaaacagc ccaggccaga
     1981 ttcagcttcg accataccga ttgcggcagg gtgagttctt tacccagcat ccgctgaatt
     2041 agcggctttt tcatcaccca ttgactgact aacagggcac ccgcaaacag ggcataaatg
     2101 acagtaacct tccatttaat aaactcatca ttgtgaaaga acagcgtcaa gccaccgaag
     2161 acgaccacca gaacaaaagt gatcagggcc atcttctcaa ccttacgaaa gcgaacccag
     2221 ctatagataa gcacaatcgc cgtggcgacg atcagcgccg cagtagccgc atagatgtca
     2281 taaatcttgt aaaacgcgaa aaagacaacc agcggtaaaa aatcaagaaa ctgcttcatt
     2341 ttatgattcc gtaatcaaat gcttaaggtt attggcgaat caacatatac aggcggaaca
     2401 gatagatgag caatacggct gaaatcaggt tgctcaaggt gttcgccagt acggcaccaa
     2461 tttccggggt taatgcggca aaagaagagg caaaaagcag caacagtgtt tttgccagca
     2521 accagctcag tactgcgggt gccaccagac gcatattcgc ccatgtcagc cgcatactgc
     2581 tacgcatcga ggcaaaaacg cccattttgt cctgtaccag catcaccggt gccagagcca
     2641 gtaaaatggc catgataatt cccggaacga ccaccagcat gatgccaatc tgtactaaaa
     2701 gggtagtcag aaaaatcaga ataaataact ttggcaatat cggcgcactg gcaccaatag
     2761 ctcgcagcgc actgactctc tgacccgcag acaccagctg gataattaat attacgcccc
     2821 cggcgagaat ggcgttaccg attaatcctg aaaaagtgga cgccgctgaa gcctgcagca
     2881 aaatttgttg ctgttccggt gacatattct gaaccaggtc gaacaaccca ctactgccgc
     2941 taacgggtac gccgtcattg agctgcgcaa gctgtgcatc actgggtgag aaaacatgcc
     3001 ctaacaccac tgtgataaac gcacatagca acgataccaa cagaatggtc ataaattgat
     3061 tacggaagaa atttccggtg tcacggtata cggactgcgc cgtgatagac atgcactctc
     3121 cttgagtttt atgcaggtgt taattagcgg gcaattgtac cctggttaag ccccgggtgg
     3181 cagcatcagg tcttgtatgg aaaagcatat ctttgtaaag cggaggtaat tgctggcctt
     3241 caggcgtggg taaaccgctg caagtggcga gcgagttaat atttgcgtag cgaaatatat
     3301 aaaaattgat ttaaatcaca ttaaccagga ttttcaatgc aacttctaaa ttaatctaga
     3361 tcaataaagg gtgaattacc atatgtaatg tgatctgtgt aggatcattt gttaccccaa
     3421 tgtgggtata ttcgtcacgt ttttataacc ataacgacgg agcggatatg aaaaagttaa
     3481 cagtggcggc tttggcagta acaactcttc tctctggcag tgcctttgcg catgaagcag
     3541 gcgaattttt tatgcgtgct ggttctgcaa ccgtacgtcc aacagaaggt gctggtggta
     3601 cgttaggaag tttgggtgga ttcagcgtga ccaataacac tcaactgggc ctgacgttta
     3661 cttatatggc gaccgacaac attggtgtgg aattactggc ggcatcgccg tttcgtcata
     3721 aagttggcac agccacaacc ggggatattg cgacagtcca tcatttaccg ccaacactga
     3781 tggcacaatg gtattttggc gattctggca gtaaattccg tccttacatt ggtgcgggta
     3841 ttaactacac cacctttttt aataatgact tcaatgacac cggtgaaggt cttggtttat
     3901 ctgaccttag tctgaaggat tcatggggcg tggcggggca agtaggcctg gattatctca
     3961 tcaaccgtga ctggttgctg aatatgtcag tctggtacat ggatatcgat accacggcta
     4021 agtacaaatc tggtgttact accgtaaaag actcggtacg actggatccg tgggtgttta
     4081 tgttctccgc aggatatcgt ttttaatttt ctctgcaaaa cctctgcaaa acccctctgc
     4141 aaaactggtc atcaaatgac cagttttttc cattccttac cgcgtgcgtc gttgtaaata
     4201 tcggtcattt tttgattcga atggcccagc aaaattttgg tatcaacccc ctgctctctg
     4261 aacaatcgct ctgataaaga tctctgctca tggaaagagg gtggggtgcc attagcacgc
     4321 cagttgtaat ccacagaatc ccgggctttt ttaaatgcaa cggttaatgt tgctggctta
     4381 accatcccgc cgcgcttagc tgtccctttc gcgtgatggt ggtgcaatag ccacggacta
     4441 agaacgcaat cgcggcagga tgacaccaca tcatctaggg tgagatttaa tttatcgcaa
     4501 cgcagagcca gagggatggc aatccgggtt cctgtttttt gctgttcgac atgaagataa
     4561 ccatcccgga tatccgaaaa ttgcattttg caaatatctg aaaggcgctg gcctgtcatc
     4621 agtgccagca gcataccgcg ctgtaaaaag taaccatcct tttccgctgc gttataaatc
     4681 atcatccact catcaaaagt cagtcgctgt cttgatatcc gcacctgcgg tttttttgcc
     4741 gattctgcag ggttaaagcc tggcgggaca tcgcccgttt gctgagcttc ccggaaaaca
     4801 tcgatcagta ccttcctgaa aatttgtccc attctgttat gtcctctggc cttgtactct
     4861 tccagtactg ataccacatc ttttacggtt atggcatcta acggtctggt gccaaaacgt
     4921 tcatcaaata ccctgagagg ggccgctttc tgtttcagcg tgttgagttt gatctcgccg
     4981 ttttcatatc tttcctgttg aatttttctg taattattca gaaaaatggt aacggttgat
     5041 gaaccgccgg tatcactaat aattttctcc tgcagactga gcatttgttc catttgctgc
     5101 cgggcaagac ggctgttcgc ttctgctgca atagtttctg ccagtttctg gtcaatactg
     5161 ccgagaccgt gatttttgcc tgttatggga tgcctgtaac gccagtaaac tttgttattt
     5221 cttttgtcaa aatacggaga taatcccgga acattggttt tatattttcg cgggcgcgcc
     5281 atcttccagt atcctcttca aagcagggtg atctgtggcg atcacctccg gcttgtttac
     5341 cattccgaca aagcgagctt gcggatccac tcgccagcgt cttccaactt ttttggggag
     5401 aggaaatatc attccggctt tagcgtattt acttaacgta ctcggagttg ggaccggttc
     5461 actgaattcc tcttttgccc actcagtgag cagaataagt cttgccatga gcgtcgttcg
     5521 ctaatcatgg tcgccgccac tatagctggt gggcaacgac cggggttgaa cattaaaaat
     5581 cagcctgatt cgggatcagt ttttgccaga taactgaaac gtattttgcc tggtaacggg
     5641 cgtcatcaag tgcattatgg cgctcacctt cgaatggaat agccgttctg gcatcgaagt
     5701 ctatggcttt ccccagctca acgattgtgc gtacatcgcg atcgttgtag taacgccatg
     5761 ggcaggggat cccctgccgt tcgtatgaac ggcgcaaaat cgtgttttcg aagttggctc
     5821 catttcccca gacctgaaca aaaaattcac cggagttttc gtcgataaat tcccgcaatt
     5881 gtaacagtgc atcatctaac gggatttcat cggtcataat ggcagactgc gcttcacgtg
     5941 attgcttcag ccacctttta atgacgtcac gatcaatgac tccgccagca gtttccagat
     6001 cgatagtctt actaaattcc ggtcccatat ctccggtttg cggatcgaaa aatattgcat
     6061 ctattgagat aatcggggca tcaggatttt ttcccatggt ttcaaggtca atcatcagat
     6121 ggtgccacaa cctgctggtg gatgtgcttt tacgatgact gttcacctta attaagggat
     6181 ttgctgtctc gcctatttca ttattgtcat tggcatgttg atcgccttcg gtgcactcct
     6241 tgttcgggag tccggcattt tctatttcct ctggatcttt ttcccgggct tcatcctggc
     6301 tttcttcgtt gaatgtctcc tggtaggttg cgtcacccat cactgcgcca caatcagggc
     6361 agttaccacc accagtctga ttgcatgcgg tacaaacttt ttcctcttcc tgttgcacga
     6421 ctggttcagg ttgtttcgtt tctggctcgt tttgtaacgc attttggctg ttttgttccg
     6481 ctttttggtc gtactgttcc gtttctgact ggttctggtg cacagaatcg cgggtttcaa
     6541 tccccttcac ccatttcgga tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg
     6601 atgcagcgag caacttatta gcgtcaggct ggctgatact ggctgcctgc ataattttgt
     6661 ttacttcgtc agcggtaact tttaccaggt ctggttgtgc ggtcgtgtca gatgcatcag
     6721 tattttgttg tgaacctgtg tatgtgccgt ttttgcgggc aaaatattct tctttcgtga
     6781 tttcagttgc cccggcagcc agcgccttat ccagaccaga aagtttgttt gcgcgaccgt
     6841 atttttcgcc atccttgtcg gtgaagagga agtagaacgg cccctcacgc tctacagatg
     6901 gttcgacttc cactttgcat tcggtttttt cgttgtccgg aattgccgtt tccactgcat
     6961 cagtttctgg tactggcgac gagagagtat cagttgcgct ctgatttctt ccttcatctt
     7021 caaacacgcc ctttgtagtc aggtattcag taatgtattt gttcagtgcc acagggtctt
     7081 tgtgaatgtc gatcggacgt tcacggacaa ggccaaaaat agtctggcgg tcgtagcgaa
     7141 gggcatcagg ctgtttgcgc attgatgccg agatacgctt ccagtcttcg cggtcgttgt
     7201 cgataacttc attttttgcc cagcgatgga tgctgccgtc aatgtttccg gcatccacat
     7261 caccaggcca gagagcgtag gccagttcgt catccagtgt tttccatgtc tgcttgtatt
     7321 cgcgatgaat ggcagcaatg accgggctga tttttcctgt tgaattttca gtgtactgtt
     7381 gattggctct ggcgcgggcg agatcaacaa cagacgtgta ttttccggtt tccttgcgtt
     7441 caccttcacg acgttttttc cagatgcgca tctctgcctg aatttcgggc catttggcac
     7501 caggcttaca tttatgctta acccacccga tggcatgcag cttaagctcc ggatacatgg
     7561 cgttaacttc tggcattttc atcaacgctt caacgatatg tccgtcgaat gttgccatgt
     7621 cttcctgcaa caattcctgt gcgctaataa ccatatcaac ggtgatgttt tcacatgtgt
     7681 cgaacttaac catgacagcg ttctgtactt caggggccag cttgtcaaaa gtgacgttca
     7741 tcggatcgga ttcagtctca accgggacaa aggaagcaga ctcctcatcc cagcggtttt
     7801 cctgcatata ttcagcatcc caggaatcga gggcagggcg gggtataccg ggtttatcct
     7861 cgcagacaag aaatttataa gcgcagtcct gagcagccgg ataatgttcc aggaattgcc
     7921 agtgaaattt tgcgcgggcg cgacgttcat caccggcttc aatggcagtg gctacagcga
     7981 cggcaccttc ttcctttatt gcctgttcgt ccggaatggc ggcgcaaata aagactttac
     8041 tcattttgtt ttacctcatt acagatttaa gggtgaacaa atccctgcca ttgctggcat
     8101 ataagaatga aaccggatat ttattacgga actgttttaa agacctgccg ggatttcgtt
     8161 attatcctgg tgaataactt tatcgaccgg gtaacagtta ccgggaattt tctgttcggt
     8221 tgctgcagtc acacactcct gcattgtcct gtgaacactg actgcaatat caactggctc
     8281 tccggaaaca agaaaaactg tcagaacaag tgcaaatgct gtattcattg ccagcatcct
     8341 ttttgtatcg gacgtaaacg ggccagcatt gaaagaatgc atattttatt taatagctcc
     8401 cgttcgtgtt ttctcttgtt aatggcatct tcagtaaata cagggttact gatattgaca
     8461 ccaatttcaa aacaaccttc agacgtatta acgtttggta ataacgtttc cattatcgcg
     8521 tcctcaacaa tgaattttgt gatgcggtgc ctggtgcctc caggtgacgt taaccagtta
     8581 acaattaacg ccggatacag agaatccacc cataacactg tttttggttt taactgttcc
     8641 gcgtgcgctc agccgcattc accacatcac aaaattcact ttaaaaaggg cggcagagca
     8701 gtcacggagt aaaactgata ccgccaaacg tcaccagaaa attgataaca gagggcgttg
     8761 cagcggagtt gtcacttaag cgtatggtca acctgacaac ccggtgtcct caacggagga
     8821 aggaataacc ccgccatact taccgccgcg ccatttcgcg gagtgccaca accggaagcg
     8881 cacggtcgaa ctaaatttaa cgacaccgta cagagagacc aatttcgccg tgcgctttcg
     8941 ctttatgccc tgacttttca gggacatatc ctttcagtaa actgtcagtg ccggatgttc
     9001 acccgtgtcc ggcgcacgca ctccacctga cccgtggaga actccttaat taccaacctt
     9061 agcttcgttg gttagctatt aacgcgggta tgtaaccatt ctggcaatgc ttaatgccgc
     9121 tgctttttcc agcctggtga tatcctgctc cagagcggac agattttcag cctgcttagt
     9181 cctggcttca ttggcccatt tcagatcctg cgctgcatta attttctggc gcatccactc
     9241 ataaagttca tcatcggtat agtctggcgc gatgatgacg ggttctcgtt tctgcatact
     9301 gattcctcgc ggtgctgctt cgcttatcag ccgttagatt ttgccgagct ggaaagcgcc
     9361 tgtttaaact cactgaagct gagagcttct tcgccttcgg caaggccttc gaagtattct
     9421 tcgtaagcct tttccatgat tgtgtcgaaa tccatatcac tcacctgagt ttctttccag
     9481 ccagcgacgg gcaccatttt cggttttaaa cgttttgctt ttggtatacg tcattgcggt
     9541 gaacgtgccg tcctggttgg ggaacacgcc acataccaga gattcgctgt tgccaagatc
     9601 gatagtatcc atgctgacct catttcccct taacgccggg ggagcggaac aaaaacctgc
     9661 tgcatagtta ttaaagttga accctgccgt catgttctta cgcctcgggc tggctactta
     9721 ccccctgacc actgcttggt aactcgaagt attgcccggc gttctgtggg gcggggtggg
     9781 ttggtatgtt gttaaggtaa caagagttac ctttcgagtc aatacaatgt tgcaaaaggt
     9841 acatttgagg gcataaaaaa cccgcaatga atgcgggttc tgactcagtc taagtattga
     9901 tgtatttgtg aaactttacc tttaatggtg taaccaccat tcagttcgat gggtttgtaa
     9961 agcggattca gtgacaacag atagatgttt ggtccgtcaa tcgcaacttt ttttagtgtt
    10021 acgtttggcg ttccttccaa ttggattaag attatttttc ccaccagttc tctaatgtta
    10081 cttgagcatg gtgtgatcag cacggtagat ccgtcgggga tggttgggag gccgttagag
    10141 tttgtcatcg catctccctc aacatgcaat aaaaaagagt tttcagcggt ttttgtcatg
    10201 acatcaaccc agttcttaat accaggaatc ttggttactg gacaactcat atcccaataa
    10261 ccagcctgtt cccacgttaa aacgggcaac cgggcgatgt tgtcactaat gtaagggtac
    10321 tgattcagac gcagatcatc ggttttatcg tgaccgtcct ttccataaag aatccattca
    10381 ggagatttgg aaagcaattt tgacagtaga tacaaattct caccgtcagg ttttgaagag
    10441 ccattttccc attttgttac ggatacacga gatatgccga ttgctttcgc aacctgctgt
    10501 tgggttaatc caacgtcttt tcgacgattc cgaatacgtt cgctgatagt gtttttcatg
    10561 taaccaatgt tactaccaag tgatgttgct atggttgaca ttgttatgta actattgtta
    10621 cccttctgct cgaaataaca ggagagtttt atgttcaaag atgatgttct gcgctatttc
    10681 aaaaaaaagc gactagtagc tgaggctctt ggaatttcac atgtggctgt tgtgcggtgg
    10741 aaagcagtta ttcccaaact tcgcgcaatg gaactggatg aaattactaa cggtgaattg
    10801 aaatacaacc cagaacttta caagaagcag gatagcacct cgaacgaagg aaagaatgat
    10861 tcatgaaaat caagcatgaa cacatccgca tggcgatgaa tg
//
LOCUS       DAPQZD010000160        10787 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPQZD010000160 DAPQZD010000000
VERSION     DAPQZD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.162"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(65..278)
                     /locus_tag="RO454_004026"
                     /pseudo
     CDS             complement(65..278)
                     /locus_tag="RO454_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(640..1833)
                     /locus_tag="RO454_004027"
     CDS             complement(640..1833)
                     /locus_tag="RO454_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0935780.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1969..3693
                     /gene="iucA"
                     /locus_tag="RO454_004028"
     CDS             1969..3693
                     /gene="iucA"
                     /locus_tag="RO454_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDY0935781.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3694..4641
                     /gene="iucB"
                     /locus_tag="RO454_004029"
     CDS             3694..4641
                     /gene="iucB"
                     /locus_tag="RO454_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDY0935782.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4641..6383
                     /gene="iucC"
                     /locus_tag="RO454_004030"
     CDS             4641..6383
                     /gene="iucC"
                     /locus_tag="RO454_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDY0935783.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6380..7657
                     /gene="iucD"
                     /locus_tag="RO454_004031"
     CDS             6380..7657
                     /gene="iucD"
                     /locus_tag="RO454_004031"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDY0935784.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            7739..9940
                     /gene="iutA"
                     /locus_tag="RO454_004032"
     CDS             7739..9940
                     /gene="iutA"
                     /locus_tag="RO454_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDY0935785.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10491..10634)
                     /locus_tag="RO454_004033"
     CDS             complement(10491..10634)
                     /locus_tag="RO454_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935786.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2602 a         2837 c         2811 g         2537 t
ORIGIN      
        1 atacgcaacg ttatggaaaa caaaaatacc ctgagtaaaa aggtccgtag gcaggcgagt
       61 acttttattt tccgctagtt ttactttccc atcgaaggcc ccatacatca ctacgcccac
      121 gaaacaaccg acgaaataac gcaactttct catccgtgga taatacggaa acgcactgca
      181 caggactctg cggtttacgt cgccattcaa tcccataagc ttcaagtgat gtcttccctc
      241 agtgctgcta attcatcact gtcagattta tgaaccatat tcgttacctg tatccgaacc
      301 actgaagcac cacagcagaa attcgggtcc cctacactga ttacagcgcc ccctctgctg
      361 cctattaact caagcacctc ccaacaactt acggaaactt aattctaata cagctgtaat
      421 tcagttgtgt cgtggtcggc cgcggttact tttttcggga agtctgctac gcatcagaat
      481 aataccgtgt tctttctgat cctgtttagc caggtagcta agggcgtaac gtaaaccagg
      541 cgggggcagc aagccagaac actgcctgac atgttttcat cagacgcagg tcctctctca
      601 tatcctctga tttactccag taaggtgatg atcatatctt tatttgatag tacggtaatg
      661 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgctg ccataatcag
      721 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      781 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      841 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      901 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      961 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
     1021 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
     1081 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
     1141 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
     1201 gctcttgcac tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1261 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1321 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1381 cagagcggcc atgcgattcc cttctccagc tctctgcaat gcccagacct gacaggctgg
     1441 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1501 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1561 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1621 ttgctttccc cagcacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1681 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1741 accgttctgt ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1801 tttttggggc gtatcttcga tccctggtga cattataatg tttcaactcc atgtattaat
     1861 tgtgtttatt tgtaaaatta atttatctga caataacatt tattattgat aatgagaatc
     1921 attattgaca taattgttat tattttgctg tgtgggagct gtttgactat gaccctgccc
     1981 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     2041 accacagact ggaaactgac agaatacccg ccagacgaat tgctcatccc gctggatgag
     2101 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     2161 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     2221 cggcttatta ttgataagct acgccatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2281 ttccaccagc gcgtgctgga aagccatgtc catacgcaac aggcaattga tgcccgtcat
     2341 gactgggccg ctctgcgtga aaaagcgttg aattttggcg aggctgagca ggcgctgctg
     2401 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2461 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2521 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2581 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2641 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaaa
     2701 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2761 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2821 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2881 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2941 gaggatggct gggccgggct gcgcgatctt aacggcaaca tcatgcagga aagtctgttt
     3001 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agactaacgt actggtctcc
     3061 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     3121 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     3181 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3241 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3301 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3361 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3421 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3481 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3541 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3601 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3661 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3721 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3781 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3841 gatcagatat ttatcgccgc tccacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3901 cgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3961 actgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     4021 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccacagggt
     4081 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     4141 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     4201 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4261 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta tttcgaaatc
     4321 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4381 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4441 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4501 ccacgcaccg ataacctgcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4561 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4621 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4681 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4741 atgaccacta ctgcattaac ctgccgggag cacaatggcg cttcatcgct gaacgtggta
     4801 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4861 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4921 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4981 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     5041 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     5101 aacgatatgc gccagagtat accaacacct tcagactgca ctggctggcg gtaaaacgtg
     5161 aacatatgat ctggcgctgc gataacgatc tggatattca gcagttgttg actgccgcaa
     5221 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacgga ctggatcaca
     5281 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgct accgacttca
     5341 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5401 cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg gggcgggctg gatatcaagc
     5461 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tacatcgctg
     5521 ccggaccact ggcttcacgc tggctacaac aggtttttgc gaccgacgcc accctggtgc
     5581 aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta tgtctcccat gaaggctatg
     5641 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5701 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5761 gcgacgaaaa caatcagccg ctggcaggcg catatataga ccgctccggg ctggacgctg
     5821 aaacctggct tacgcaactg ttccgggtgg tcgtggttcc tctgtatcac ctgctttgcc
     5881 gctacggtgt cgcgcttatt gcacatggac aaaatataac tctcgccatg aaagaggggg
     5941 ttccacagcg tgttctgctg aaagacttcc agggcgatat gcgactggtg aaagaagagt
     6001 tccccgaaat ggactctttg cctcaggagg ttcgtgatgt tacatcccgc ctgagtgcgg
     6061 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     6121 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     6181 tgagtgatta catgaaaaaa catccacaaa tgtcagagcg ttttgcgctt ttctcacttt
     6241 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg cctgatctgg
     6301 atggcggcag ccgcatgctg ccgaattacc ttgaggatct gcaaaatccg ctgtggctgg
     6361 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtag ggacagggcc
     6421 atttaatctc agcattgctg cgttgtcaca tcagatcgaa gaactggact gtctcttctt
     6481 tgatgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6541 gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct acaaatccct acagttttgt
     6601 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6661 atcccgtgaa gagttttctg actacctccg ctgggctgct gaagatatga ataacctgta
     6721 tttcagtcat accgttgaaa acattgattt cgataaaaaa cgtcgattgt ttctggtgca
     6781 aaccagccag ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6841 tttaccaccc tgtgtgaagc atatgacaca atcctgtttc catgccagtg aaatgaatct
     6901 tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt ggtggaggac agagtggtgc
     6961 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     7021 ctcccggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     7081 acctgaatat atttcaggct tctccggact ggaggaagat attcgccatc agttactgga
     7141 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     7201 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7261 ctcggtaaca actctggaaa gtagtggtcc tggctggaag ttgctgatgg agcatcatct
     7321 ggatcagggc agggagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7381 tgcgttgcca caaatacttc cctcactgat gcccctgatc accatgcacg ataagaacac
     7441 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaga acaacatctt
     7501 cgtggtcaac gccagtatgc aaacccatgg catcgccgaa ccccagctca gcctgatggc
     7561 atggagatct gcacgtattc ttaatcgcgt aatgggacgt gatttattcg atctcagtat
     7621 gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga aaacgcagcc ggaggctgct
     7681 tctttaactc gctacacagc atctttgggc tgattttttc cgcccgtatg gaggaataat
     7741 gatgataagc aaaaagtata cgctttgggc tctcaaccca ctgcttctta ccatgatggc
     7801 gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg gtgtctgcca accgcagcaa
     7861 tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc gaaaacgccg aactggaaca
     7921 gcagattcag ggcggcaaag agcttaaaga cgcactggct cagctgatcc ctggccttga
     7981 cgtcagcagc cggagccgca ccaactacgg tatgaatgtg cgtggccgcc cgctggtcgt
     8041 gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac agccgacaac tggactctat
     8101 agatcctttt aatatcgacc atattgaagt gatctccggt gcgacgtccc tgtacggcgg
     8161 cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa ggccagccgg aaaccatgat
     8221 ggagtttgag gctggcacca aaagtggctt tagcagcagt aaagatcacg atgaacgcat
     8281 tgccggagct gtctccggcg gaaatgagca tatctccgga cgtctttccg tggcatatca
     8341 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ttgcttgata acacccagac
     8401 cggcctgcag tactccgatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8461 atcccggcag cttcagttga tcacacagta ctataaaagc cagggcgacg acgattacgg
     8521 gcttaatctc gggaaaggct tctctgccat cagagggacc agcacgccat tcgtcagtaa
     8581 cgggctgaat tccgaccgta ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8641 tgacagcgct tttctgggac aggagctggt cggtcaggtt tactaccgcg atgagtcgtt
     8701 gcgattctac ccgttcccga cggtaaatgc gaacaaacag gtgacggctt tctcttcgtc
     8761 acagcaggac accgaccagt acggcatgaa actgactctg aacagcaaac cgatggacgg
     8821 ctggcaaatc acctgggggc tggatgctga tcatgagcgc tttacctcca accagatgtt
     8881 cttcgacctg gctcaggcaa gcgcttccgg agggctgaac aacaagaaga tttacaccac
     8941 cgggcgctat ccgtcgtatg acatcaccaa cctggcggcc ttcctgcaat cagactatga
     9001 catcaataat ctctttaccc tcaacggtgg cgtacgctat cagtacactg aaaacaagat
     9061 tgatgatttc atcggctacg cgcagcaacg gcagattgcc gccgggaagg ctacatccgc
     9121 cgacgccatt cctggcggct cagtcgatta cgacaacttc ctgttcaacg ccggtctgct
     9181 gatgcacatc accgaacgcc agcaggcatg gctcaacttc tcccagggcg tggagctgcc
     9241 ggacccgggt aaatactatg gtcgcggcat ctatggtgct gcagtgaacg gccatcttcc
     9301 tctaacaaag agtgtgaacg tcagcgacag caagctggaa ggcgtgaaag tcgattctta
     9361 tgagctgggc tggcgcttta ctggcaataa tctgcgcacc caaatcgcgg cctactattc
     9421 gatttctgat aagagcgtgg tggcgaataa agatctgacc atcagcgtgg tggacgacaa
     9481 acgccgtatt tacggcgtgg aaggtgcggt ggactacctg attcctgata ctgactggag
     9541 taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa gtgaacggta cctggcagaa
     9601 atacgatgtg aagacagcaa gcccatcaaa agcgacagcc tacattggct gggcaccgga
     9661 cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac gtgagcgacg cgcagggcta
     9721 caaggtcgat ggctatacca ccgtggatct gctcggcagt tatcagcttc cggtgggtac
     9781 actcagcttc agcattgaaa acctcttcga ccgtgactac accactgtct gggggcagcg
     9841 tgcaccactg tactacagcc cgggttacgg cccagcgtca ctgtacgact acaaaggcag
     9901 gggccgaacc tttggtctga actactctgt gctgttctga cggggatgct tttgcaacaa
     9961 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10021 cagccttaac aagatattcc gtctccggaa tggactgttc accactgaat aacattatgt
    10081 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10141 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctggg
    10201 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10261 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10321 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10381 attgttaata cgatggtgca cgatgcattg tcagtgcccg gcccggagga tgccccgtag
    10441 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10501 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gcgatggaga
    10561 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10621 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10681 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattagt attgattcac
    10741 ataatctctc atcattaatg agaacattgt cacatattta caatata
//
LOCUS       DAPQZD010000161        10769 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPQZD010000161 DAPQZD010000000
VERSION     DAPQZD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.163"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(87..1133)
                     /gene="aroH"
                     /locus_tag="RO454_004034"
     CDS             complement(87..1133)
                     /gene="aroH"
                     /locus_tag="RO454_004034"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDY0935787.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1290..2123)
                     /gene="ppsR"
                     /locus_tag="RO454_004035"
     CDS             complement(1290..2123)
                     /gene="ppsR"
                     /locus_tag="RO454_004035"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDY0935788.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2456..4834
                     /gene="ppsA"
                     /locus_tag="RO454_004036"
     CDS             2456..4834
                     /gene="ppsA"
                     /locus_tag="RO454_004036"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDY0935789.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4891..6537)
                     /gene="fadK"
                     /locus_tag="RO454_004037"
     CDS             complement(4891..6537)
                     /gene="fadK"
                     /locus_tag="RO454_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDY0935790.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6594..6887)
                     /locus_tag="RO454_004038"
     CDS             complement(6594..6887)
                     /locus_tag="RO454_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDY0935791.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(6884..8173)
                     /locus_tag="RO454_004039"
     CDS             complement(6884..8173)
                     /locus_tag="RO454_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDY0935792.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8228..9166)
                     /locus_tag="RO454_004040"
     CDS             complement(8228..9166)
                     /locus_tag="RO454_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDY0935793.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(9186..9950)
                     /locus_tag="RO454_004041"
     CDS             complement(9186..9950)
                     /locus_tag="RO454_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDY0935794.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10266..>10769
                     /locus_tag="RO454_004042"
     CDS             10266..>10769
                     /locus_tag="RO454_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDY0935795.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         2598 a         2741 c         2785 g         2645 t
ORIGIN      
        1 ttatggaggg ccagagcaag cagtaatttg ttgagaagac aacaagttgc gcagaaatgc
       61 ccatcctgtc aggaatgggc acgcattcag aagcgggtat ctaccgcaga ggcgagtttt
      121 tcgaccaggc gttcggtatc ttcccagccc agacacgggt cggtaatgga ttgaccgtaa
      181 gtgagcggct gaccgccgac gattttttgc gttccttcgc gcaggaaact ttccgccata
      241 attccggcaa tcgccgtaga gccattgcgg atttgctgac aaatatcctc acaaacttct
      301 aactggcgac ggtgctgctt ctggcagtta ccgtggctga aatccaccac cagatgttca
      361 ggcaaatcaa attcgtgcag cgtatcgcag gctgcggcga tatcatcggc atgataattc
      421 ggttttttgc cgccacgcat aataatgtgg ccatacgggt tgccgctggt ctgatagatg
      481 gtcatctgac cgtttttgtc tggcgagagg aacatatggc tagcgcgggc tgcgcggata
      541 gcatccacgg caatccgcgt gttgccatcg gtaccatttt taaaacccac cggacaggag
      601 agtgccgaag ccatttcgcg gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc
      661 caactgatta aatcagcaat aaactgaccg gtcaccatat cgaggaactc agtcgcggta
      721 gggacgccca gctcatttac ctgtaaaagt aatttgcgcg ccagctccag accgtgattt
      781 acccgatagc taccgtttaa atctggatcg gagatgagtc ctttccagcc gacaaccgtt
      841 cgtggttttt caaaataggt gcgcattacg atttccagcc gtgactggta ctggttgcgc
      901 agcgactgca gacgggtggc gtactccatc gcagcggtga gatcgtggat cgagcagggg
      961 ccaataatga ccaacagtcg cttatcttca ccattcagta ttttttcaat tctacggcgg
     1021 gagtcggtga catgggtggc gacgccaggc gttacgggat accgtagcgc gagttcggcg
     1081 ggcgttacca ggctctcaat acgcgcagta cggagttcgt cagttctgtt cattacaagt
     1141 ctcagaaatt tgttgcttct acggcaggac taccgggaag tgatgcgcat cacgataaac
     1201 caatcgcagc tgatttcaag tgcgaggtgt gtcacacctc gccgggtggt tagcatgata
     1261 acaaaaaaac aagctaatgc actagttctc tagtacattc ggcggctaag gcccatgata
     1321 tcgaggatct tggtggcaat ctcttctacc gaataattgg tactgttaat ccacgggatc
     1381 tgatttttac ggtacaacgc ttccacttcc gcgacttcca tcctgcactg acgaagcgag
     1441 gcatagcgac tgttctcccg acgttcctcg cgaatcgccg ccagacgttc cgggtcgata
     1501 gtcaggccga acaatttatg ctgaagtggt ttgagcgacg cgggtagcac cagattatcc
     1561 atatcatcgg caataaaggg gtagtttgcc gcgcggatac caaattgcat tgccagatac
     1621 agactggtgg gggttttacc acagcgagaa acaccgagca ggatcacctg agcctggtcc
     1681 agattgcgca acgaaatacc gtcatcgtgg gcaagggtgt aatcaatcgc cgcaatgcgc
     1741 gcatcatatt tattgagatt attagggtta aggccatggg tacgatgagc aatcggcgtt
     1801 ggatccagtt tcatctcttg ttgtagtggg gcaaccagcg cctgaacgat atcctggcaa
     1861 aagccttcac tttgcaagat gatagcgcga atctccggca acacgatgga gtagaagacc
     1921 agcgggcgca cacctgtctg gtgataaatt gcgtcaatct gatccttcac tgcccgtgca
     1981 cggctctcat tttcgacaaa cggcagcgta atgctgctga tagtgacggg aaattgtgac
     2041 attactgcgt gtcctaatac ctccgcagtt attgccgtac catcagaaat ataaaaaacg
     2101 tggcgatcaa cagcattatc cattttgttc ttcccgtgat gcagacataa ttctatgaaa
     2161 gcataaatta aaaacgcaca gaagcgtaga acgttatgtc tggtttataa aatgaaactt
     2221 aaattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat accgatggtt
     2281 tatgtggaaa ttgtcgaaga gagcagattt gcgcaacgct gggatcagtc ttaaaaagta
     2341 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ctggttaaat atgcaaagat
     2401 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
     2461 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
     2521 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaaccttt ccggaatggg
     2581 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
     2641 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
     2701 ccagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
     2761 tgagctggaa aacgccatcc gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
     2821 ttcgtttgcg gtgcgttcct ccgctactgc agaagatatg ccggacgctt cttttgccgg
     2881 tcagcaggaa actttcctca acgttcaggg tgttgacgcc gttctcgtgg cagtgaagca
     2941 tgtattcgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
     3001 ccatcgtggc gtagcgctct ccgccggtgt tcagcggatg gtgcgctccg acctcgcatc
     3061 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
     3121 ttccgcatgg ggccttggtg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
     3181 cgtccataaa ccgacacttg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
     3241 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
     3301 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
     3361 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc
     3421 gaaagatggt cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
     3481 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
     3541 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gttatccatg acatcagtga
     3601 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
     3661 accgatcatg aagaaagcat ctgccatcgt caccaatcgt ggcggtcgta cctgtcacgc
     3721 ggcgatcatc gctcgtgaac tcggcattcc ggcggtagtg ggctgtggtg atgcaacaga
     3781 acggatgaaa gacggcgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
     3841 ctatgcggag ttgctggaat ttagcgttaa aagctccagc gtagaaacga tgccggacct
     3901 gccgttgaag gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
     3961 gccgaacgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
     4021 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
     4081 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
     4141 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
     4201 taaatcgaac gaatatgcca atctggtcgg tggtgagcgt tacgagccag atgaagagaa
     4261 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
     4321 cgcgctggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ctaacgttga
     4381 gatcatgatc ccgttcgtgc gtaccgttga tcaggcgaaa gcggtggttg aggaactggc
     4441 gcgtcagggg ctgaaacgtg gtgagaacgg gctgaaaatc atcatgatgt gtgaaattcc
     4501 gtccaacgcc ttgctggcag agcagttcct ggaatatttc gacggcttct caattggctc
     4561 aaacgacatg acgcagctgg cgctcggtct ggatcgtgac tccggcgtgg tgtctgaact
     4621 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggcgattc gtgccgcgaa
     4681 gaaacagggc aaatatgtcg ggatttgcgg tcagggtcca tccgaccacg aagactttgc
     4741 tgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
     4801 aacctggtta agcctggctg aactgaagaa ataaaataaa tacccggcgg cgttttgtcg
     4861 ccgggtttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgagt
     4921 taatcgccgc ataatatctt tccttaacaa aaacttttgt attttacctg agacagtgcg
     4981 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
     5041 gctaaaaaaa gccaccacct cttccagcga taatgaatga tgcggcgctt tcagcacgac
     5101 ataagcgcat gaacgttcac ctaaacgttc atcgggcatc gcaaccacac aggcatcgtg
     5161 aattttagga tgctgcaata aaatatcttc tacttcacgg ctgctaatat tttcgccgcc
     5221 gcggacaata atatctttct tgcgcccggt tatttttata tagccagcct catccatgcg
     5281 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
     5341 ttcatcaaaa taccccataa acacattggg gccacgcgag gcttcttcac cttcgcaacc
     5401 tggcggtaaa gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
     5461 accgtcggtg tgcataaagc gcggcaaagg atcgtcgaga ttcaccaccg catgcggcga
     5521 actttctgtg gaaccataaa cacttaataa tttaatgccg cgctgctggc attctcgcgc
     5581 cacttttttg gggattgtgg tacctccgca aagaaagaag cgcagcgctg aaaggtctgc
     5641 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc cgagcataca
     5701 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
     5761 cacgctgcga gcaccaatca aaaaaggtgc tgttacgcca tgcagaaagc ccgttgcgtg
     5821 accaagtggc gcaggcatca taaagacatc ctgccaggtc agatttagcc gcgcgcaata
     5881 agcccgctca ctggcgagaa tattgttatg cgttagcatc acaccctttg gcagaccctc
     5941 ggttccggag gtaaatagca cagcggctaa ttcatcgccg tgggtcgtta tcgccgtggt
     6001 cagaggagta ttgtcggcga gaatctgact taatgagagg gaagaggtgg cgggagccag
     6061 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
     6121 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
     6181 attgagcacc cacaccagtt ctgcttcccg ccaggaaggt aacagcggca cggaaaccgc
     6241 accggttttc aggcaggcaa gatagataac ggtaaattca caccagcctg gcagttgaaa
     6301 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
     6361 ggcgtgatcg agcgcgctat aggtgtacgt tgcaccatga ttatcgacca cggcaatttt
     6421 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agcgaagcat cgccccataa
     6481 cccttgctga cgatacgccg cacgacgttg ttcgttgaac gttaatgtca ctttcatttt
     6541 ggactctcga aacagaacat caggcccgag atgcgggcct gtgggatgca tgattagccg
     6601 tagcgaaatt caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgttg
     6661 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cggaatcgaa atgaatgttt
     6721 cctgcgtcat cctgcttata aagtccggca gggcaagctt tcattaattt acggaattca
     6781 ttgatatcgg gatttgccgc caaaatgata tgcgggtggc cctcatcaac atggaattta
     6841 tcgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
     6901 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
     6961 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catatcgttc atgatgtcgg
     7021 cgaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
     7081 ggaaatgctg catatcgcgc ataacacaac tttgttccag ctcacgtttg tattgcgcca
     7141 gactgctggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
     7201 gagctgatgc aatggctaaa tccatgccgc gaactgtaaa acccaaattc aggcagaagc
     7261 ctgcggcgtc accaacgatc atcacgccat cgttaaccag ctgcggcacc attgccagac
     7321 cgccctccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
     7381 gaatagcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
     7441 cgatatcacc caatccacaa accagcccca acgatacgga atccttgttg gtatagagga
     7501 atccgccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
     7561 cgcccgtaac attaaagcga tcgttgatct gttctggtgt caggcctata acctctttaa
     7621 caccaacagc gtaatgatgc ggattggaag cggaaaccat tcccagcgag cggccaagca
     7681 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccggcct
     7741 ggacgccagt gaccttgttt ccttcacgaa ccagcgcatc gacgcgtacg cccgggataa
     7801 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
     7861 cggtataaga cgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
     7921 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
     7981 atgctgcaaa gcctggaata attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
     8041 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgcg
     8101 ccatgaccag tgcggcaacg ctaccggcaa cgcccgcacc gaccacaatg gcatcaaatt
     8161 tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag atgtggttga
     8221 aagggaatta gcgattcaac tggctaatca gggcagggac gactttgtag atatcgccca
     8281 ccagaccgta gtcggcatag ttgaagattg gcgcattttt atctttatta atggcgacaa
     8341 tcacttttgc gccgttgccg ccaaccatat gctggatctg cccggagatc cccagcgtca
     8401 ggtagagatc ggatttcagc aacacgccgg agacaccgat ataacgttca cgctccatcc
     8461 agttctcgcc ttcggcaatt ggacgtgaac agccgacttc agcattcagc actgccgcca
     8521 gttcgtggac cattttcagg tcatcctgcg ccgccagacc acgaccgacg ccaaccacac
     8581 gttttgcttt gctcaggtcc acgctgcttg ccgctttcgc acggcgttcg cgacagagaa
     8641 tttcatgacg cggggcaaca taaggtaccg tttctgtcgg gcactgatga gagatatcac
     8701 gggtgcacgg ttcctgaaca ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct
     8761 tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg tgaccatcga
     8821 caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg
     8881 cggccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt
     8941 tcagcagggc agcaatgctt tcggcgtaat tttcagtacg ttgcagcgcg tcgttttgct
     9001 caagaacata aatgcatttt ggcccataag gcataactgc ctgcgcctgc tcggtatttt
     9061 gtacaatggc atacacctgt tggccccatt gctgagcgct gccaaacagt tcagcataac
     9121 gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt tcttctccgt
     9181 aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc ctccggcgaa
     9241 tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat gccggtcagt
     9301 tcagcaagtg gtgcgctctg gctccagcca atatcacttg cctgccactg atttaccggt
     9361 tttttacccg cgccgagaat ggctttcatc gaaggaatac gtggcacgtt aatatcggag
     9421 gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc ttcaagcgtg
     9481 cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt aatcaccgga
     9541 agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc gccggaacct
     9601 tcaccaaaga tcagtaaatc gaagccgatc ttttcaactg ctgtcgccag cgcctttgcg
     9661 gtatcgagag gcagtgcatg ctcaagttgt gcatcctgca ccatatacag gctgtgcggc
     9721 ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaaagagcc gccgacggtc
     9781 agcgcggcta tctcatcgtc atctgttgca agctggcttg cagcttcaat ggcattgaga
     9841 tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc tggagtgaca
     9901 acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat gatttctcct
     9961 gtgagtcgca tacggaacca tctccgatgg aaagtaaaaa tggcctgaaa atagtgtgcg
    10021 atagcggtgc ggggttttcg acggagcaag ctgttttcct tgccaacaat cttgcaaaaa
    10081 aaatgcgatc gctaaaacaa aagtgcaatt tgcggcaata tagttattcc atgaccgtac
    10141 tatcttgcat tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt tgtataaccg
    10201 caatacacgc cgtattttat ccggcatata ttgtttttgc ttgctcatgg aatagggagg
    10261 ttgtcatgta tcaacgctgt tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa
    10321 cgccacggct ttcacgtttc gcatttagcg atgatccaaa atgggagtct ggacatcacg
    10381 ttcatgacaa tgaaaccgag ctgatttacg tcaagaaagg ggtagcaagg tttaccatcg
    10441 attcttcgtt atatgtcgcg catgccgatg acattgtggt gatagaacgt ggcaggttgc
    10501 atgcggtggc ctctgacgtt aacgatccgg caacgacgtg tacctgtgcg ctgtacggct
    10561 ttcagtttca gggggctgag gaaaatcagc tactgcaacc gcattcttgc ccggtaattg
    10621 ccgcaggaca gggaaaagaa gtcattaaaa ccttatttaa tgagctaagt gtgattttgc
    10681 cgcaaagtaa aaatagccaa acatcttcgt tatgggacgc atttgcctat acgttagcaa
    10741 ttctttacta cgaaaacttt aaaaatgct
//
LOCUS       DAPQZD010000162        10730 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPQZD010000162 DAPQZD010000000
VERSION     DAPQZD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.164"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            314..577
                     /locus_tag="RO454_004043"
     CDS             314..577
                     /locus_tag="RO454_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDY0935796.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            577..717
                     /gene="ykgO"
                     /locus_tag="RO454_004044"
     CDS             577..717
                     /gene="ykgO"
                     /locus_tag="RO454_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDY0935797.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(787..977)
                     /locus_tag="RO454_004045"
                     /pseudo
     CDS             complement(787..977)
                     /locus_tag="RO454_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1801..2343
                     /gene="ecpR"
                     /locus_tag="RO454_004046"
                     /gene_synonym="matA"
     CDS             1801..2343
                     /gene="ecpR"
                     /locus_tag="RO454_004046"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDY0935798.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            2418..3005
                     /gene="ecpA"
                     /locus_tag="RO454_004047"
                     /gene_synonym="matB"
     CDS             2418..3005
                     /gene="ecpA"
                     /locus_tag="RO454_004047"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDY0935799.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            3062..3730
                     /gene="ecpB"
                     /locus_tag="RO454_004048"
                     /gene_synonym="yagY"
     CDS             3062..3730
                     /gene="ecpB"
                     /locus_tag="RO454_004048"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDY0935800.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3756..6281
                     /gene="ecpC"
                     /locus_tag="RO454_004049"
                     /gene_synonym="yagX"
     CDS             3756..6281
                     /gene="ecpC"
                     /locus_tag="RO454_004049"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDY0935801.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6271..7914
                     /gene="ecpD"
                     /locus_tag="RO454_004050"
     CDS             6271..7914
                     /gene="ecpD"
                     /locus_tag="RO454_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDY0935802.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            7883..8593
                     /gene="ecpE"
                     /locus_tag="RO454_004051"
     CDS             7883..8593
                     /gene="ecpE"
                     /locus_tag="RO454_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDY0935803.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8900..9229
                     /locus_tag="RO454_004052"
     CDS             8900..9229
                     /locus_tag="RO454_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDY0935804.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(9241..9404)
                     /locus_tag="RO454_004053"
                     /pseudo
     CDS             complement(9241..9404)
                     /locus_tag="RO454_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(9478..10092)
                     /locus_tag="RO454_004054"
     CDS             complement(9478..10092)
                     /locus_tag="RO454_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDY0935805.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(10307..10492)
                     /locus_tag="RO454_004055"
     CDS             complement(10307..10492)
                     /locus_tag="RO454_004055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935806.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            10541..10699
                     /locus_tag="RO454_004056"
                     /pseudo
     CDS             10541..10699
                     /locus_tag="RO454_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2927 a         2650 c         2563 g         2590 t
ORIGIN      
        1 ccttttttgt aagtaattat tatatccgtg ggagaggaat acacattgtc aggtaatcaa
       61 tcatgctgca ataaatcatc ggccagtaaa gtggagatag cctccattct cgaaaaatcc
      121 atactctcag cgaaaccatc atcaatcact catccaggcg tttatgggag cgtcgccaat
      181 ggctgctaac aatgccagac ttccccgttg cggaaattcc acatcccaca aatagtcaca
      241 gtgattgggt gttgaaatga tccggatgag catgtatctt tacggttatg ttataacata
      301 acaggtaaaa atgatgaaac ccaatatcca tcctgagtat cgtactgtgg tgttccacga
      361 caccagcgtt gatgagtact ttaaaatcgg ctcgactatc aaaacagacc gtgagattga
      421 gctggatggc gtaacgtatc cgtacgtaac aattgatgtc tcttcgaaat cgcacccgtt
      481 ctatacaggg aagctgagaa cagtggcatc agaaggaaat gttgcacggt tcacccaacg
      541 ttttggtcgt tttgttagca cgaaaaaggg ggcatgatga aagtccttaa ctctctgcgt
      601 accgcaaaag aacgccatcc agactgtcag attgtgaagc gaaaaggacg gctgtatgtg
      661 atttgtaaat ctaatccacg ttttaaggcc gttcagggtc gtaagaaaaa acgttgattt
      721 aaaattcgac gaattaacga tatttgtctg attaataatc agaccggatt aatgttggtg
      781 tatttattac accagcatta attttcctgg ggatgtatct tcctgttcat ttaaggtcaa
      841 ctgcctgacg tttctctccg aatattccat tatattaatg ttgacttgtt gaccagcttc
      901 gccctgtatg ctggcatcaa ctctctttta gactgaacac gccactcagt ttcctccctt
      961 tgcgacgcag cctgcattta cactcaaact gttaagatga taaatgtggt aaatctgttg
     1021 gtactgacat aaaaacgttt acgccacagg aacggccaga tccatcggta accccatcgc
     1081 cgacgttcga gtgccagtta gagtaacgcg cacagatgac tgaatgcagt gccctggcaa
     1141 agaggccatc gttcctgtga caactggcag tcttcgttta acttcactta atttagctct
     1201 tagggggctt accggacaga tgacgtactt acacctgttt aattttccat cacttattgg
     1261 aatgaacacc cataaccatt ttgtgcggca tgttaatcca ttaaaacacc ttactgattg
     1321 gcaaatcatc tttaattatg acttatgata gttttatatt ctatttctta tcatttaaac
     1381 ttgttttttt actagtccat tacacaacac attaagacta ttcctaacac ctcagggcaa
     1441 agttcctggc taatataaaa tgcaagtaag aattgaacgt tatattgcca ataaccttat
     1501 gaaactgaat gtctttttct tcttatcaaa aaagcaatat tttcattttt tgtaaatatt
     1561 gacttaacca tggaattcat tttctgttca catattgaca ctcatcagga aaaaaacata
     1621 aatttaaacc taatcgaaat aattaaaact taatctcgtt taacctatat tgatatgtgc
     1681 tacgtatctt atttacttcc gatttactaa aaaaactgaa tgtacctgta aaaattacag
     1741 gtttggaaag tagtgacatg gcaaaatgat tacagcaggg actatgaggt taaaaaccat
     1801 atggaatgtc aaaaccgttc tgataaatac atctggtctc cccatgacgc ctacttctat
     1861 aaaggactat ctgaactgat tgtggatatc gacagattaa tttatctatc gctggagaaa
     1921 atcagaaaag atttcgtgtt tatcaatctc aatacggatt ctttaactga gtttataaac
     1981 cgtgataatg agtggttatc cgcggtaaag gggaaacagg tcgtattgat tgcggccaga
     2041 aagtcagaag ccttagcaaa ttattggtat tacaacagca atattagggg cgtggtatac
     2101 gctggactga gtcgtgatat tagaaaagaa ctggcctatg tgattaatgg caggttcctg
     2161 agaaaagata ttaagaaaga taaaatcact gaccgggaaa tggaaattat ccgcatgacg
     2221 gctcagggaa tgctgcctaa atcgattgcc agaattgaaa attgtagtgt gaagacagtg
     2281 tatacccatc ggcgtaatgc agaggccaag ctgtactcaa aattatataa gttggttcag
     2341 taaactccag gcaagttagt tttaaaaaat gactcaccgg gacttcatgt cctcaattca
     2401 actcgggaag aaaagcaatg aaaaaaaagg ttctggcaat agctctggta acggtgttta
     2461 ccggtacagg tgtggcgcag gctgctgacg taacagctca ggctgtagcg acctggtcag
     2521 caacagccaa aaaagacacc accagtaagc tggttgtgac gccactcggt agcctggcgt
     2581 tccagtatgc cgaaggcatt aaaggtttta actcacaaaa aggtctattt gacgtggcta
     2641 tcgagggtga ctcaacggct accgccttta aactgacctc acgtcttatc accaacacct
     2701 taacccagtt ggatacctca ggttccacac tgaatgtggg cgtggattat aacggcgcgg
     2761 cagtcgaaaa aactggcgat accgtgatga tcgataccgc caacggcgta ctgggcggca
     2821 accttagccc actggctaac ggttacaatg ccagcaatcg taccactgca caggatggtt
     2881 tcactttctc catcatcagc ggcaccacca atggtaccac cgcagtaacc gattacagca
     2941 ctctaccgga aggcatctgg agcggcgacg ttagcgtaca gttcgacgct acctggacca
     3001 gttaatctct ctgatgtacc agcaggggta cccccctgct ttattcctgg acggaattgt
     3061 tatgaaaaag caccttctgc ctctcgctct gctgttttcc ggaatatcac cggcccaggc
     3121 gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc tgagcaaaac
     3181 gatccaaaac agtaccgaca gtggccgcct catcaatatc cgtctcgaac ggctctcttc
     3241 accgcttgac gacgggcagg ttatcgcaat ggacaagccg gatgagttgc tactcactcc
     3301 cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc
     3361 agcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc
     3421 gcagcgcgat aatgccaacc gcagcgctgt ggccactgcc tccgcccgca tcggcaccat
     3481 cctggtcgtc gcaccccgcc aggcaaacta ccactttcag tacgccaacg gctccctgac
     3541 aaatacagga aatgcgacgc tgcggatcct cgcctacggc ccctgcctga aagccgccaa
     3601 tggtaaggag tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg
     3661 cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc
     3721 cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag
     3781 gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg
     3841 ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg
     3901 cgctccagga cggcatgagc gtcccgcttt atattcatct cgccggtagc cagggtcgcc
     3961 aggacgatca gcgaatcggc agcgctttta tctggttgga tgatggacag ttacgcatcc
     4021 gaaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc
     4081 agctgatgac tctggccaac gccccgttca atgaggccct taccatcccc ctgactgaca
     4141 acgcgcagtt ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag
     4201 cgctgggcac cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc
     4261 tcagcagtaa tctgagctat aacttcggcg tctataacaa ccagttgcgt aacggcggga
     4321 gcaacacctc cagctatctg tcgctgaata acgttactgc gctgcgcgaa catcacgtgg
     4381 tgctcgacgg ctcactgtac gggattggta gcggtcaaca ggacagtgaa ttatataaag
     4441 cgatgtatga acgcgatttt gctggtcacc gatttgccgg tggaatgctc gacacctgga
     4501 acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct
     4561 ggggaaacca ggccagctcc accatcttcg acagcagcca gtcggccacg ccagtgatcg
     4621 cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc
     4681 agaacttcac catgggcaat catgaagtgg atacccgggg tctaccgtac ggtatttacg
     4741 atgtggaagt tgaggtaatc gttaacggtc gcgtgatcag caaacgcacc cagcgggtca
     4801 ataagctgtt tagccggggg cgtggcgtcg gtgcaccgct ggcgtggcag atatggggcg
     4861 gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gccaaagaga
     4921 gttggctggc aggtgcctcg acctccggct cactgagtac gtttagctgg gcggcaacgg
     4981 gatatggata cgataatcag gcggtgggtg aaacccgtct gacgcttccg cttggggggg
     5041 cgatcaacgt taacttgcaa aacatgctgg ccagtgacag ctcatggagc aacatcgcca
     5101 gcatcagcgc cactctacct ggaggcttca gttcgctgtg ggttaaccag gaaaaaaccc
     5161 gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca
     5221 acctgaactc actgtggtcg aagctgggta cgttcagcat cagctacaat gatgaccgcc
     5281 gctacaacag ccactattac acggcagatt actatcaaag tgtctacagc ggtacctttg
     5341 gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc agcgccaata
     5401 cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga
     5461 tgacccatca aaacggctac accatggcaa acctgtcagc acgcaaacag tttgatgaag
     5521 gaaccattcg cactgttggt gccaatctgt cacgagccat ctccggcgat accggtgatg
     5581 acaaaaccct cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc
     5641 tgaacgtcaa tagcgcggcg gacggctaca tcaatactaa tttgaccgcc aacggcagcg
     5701 tcggctggca gggtaaaaac atcgctgcca gcgggcggac cgatggcaac gctggggtga
     5761 tattcgacac tggcctggag aacgatggtc agatcagcgc caaaatcaac gggcggattt
     5821 tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg
     5881 agttgcagaa cagcaaaaac tcactcgaca gttacgatat cgtcagcggt cgcaaaagtc
     5941 atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta
     6001 ccgtctccgg tcgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca
     6061 accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggac gtggataaga
     6121 aataccccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gcactggaac
     6181 tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt
     6241 gggcggcggt gacgcagaca ggagaagaga atgagagtta acctactgat agcgatgata
     6301 atctttgcgc taatctggcc tgcaaccgcg ctcagagcgg cagtgagcaa aacaacctgg
     6361 gcggatgctc cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc
     6421 gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta
     6481 aaatacaatg gttcaggaac catctatcag caaagcctcg gctacattga taacggttac
     6541 aacaccggcc tttataccaa ctggaagttt gatatgtggc tggaaaattc accagtttca
     6601 tctcctttaa ctggcttgcg ctgcatcaac tggtacgcag ggtgtaatat gaccaccagt
     6661 cttatcctgc cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc
     6721 ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt accagtatct gcaacaaatg
     6781 cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac
     6841 gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc
     6901 acccatacga aagcagcaaa tctgcggttg ataaataccc actcgctggc ggaagtattt
     6961 atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg gacgcaaacc
     7021 atcggcagcc gttcaggatt aagttgtaag atggttaact ataccctgca aacaaacgga
     7081 ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc
     7141 gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat
     7201 actgcctatt actacacctt caacgagatg aagagcgcag actcgatcta tgtgttcttc
     7261 tcgagcaact tctttaagca gatggtgaac ctcgggatca gcgatatcaa caccaaagat
     7321 ctgttcaact ttcgctttca gaacaccaca tcaccggagt ctggctggta tgaattttct
     7381 acctccaaca cgctgattat caaaccccgt gatttcagca tcagtattat ctccgatgaa
     7441 tatactcaga caccgtcgcg ggagggatat gtcggcagcg gcgagtcggc actcgatttc
     7501 ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc
     7561 ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag
     7621 gcgaaagtac cgttcccggc gacgctttcc tttattaccc gcaacggagc tacaaaaacc
     7681 tacgatgccg ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg gttgaccaca
     7741 ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg
     7801 attccaatgg ataacgccat ttctctgcgt acggtagatg ataacggctg gtttggcgaa
     7861 gtcagcgctt caggagagat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct
     7921 gctgacagtg gtctgtatgc tggcggcacc tttgacacag gcgatttcgg tcggcaatct
     7981 gactttttca ctgccgtccg agactgactt tgtcagcaaa cgtgtcgtga ataacaacaa
     8041 aagcgcacgg atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt
     8101 gcgcacccga ccggtggatg gtgaactact tttcgccccc cgccagctgg cgttgcaggc
     8161 tggtgagagc gagtatttta aattttacta tcatggccca cgggataacc gcgagcgcta
     8221 ctaccgggtc tcatttcgcg aggtccccac tcgtaacctg acaaaacgca gcccaagcgg
     8281 cggtgaggtc agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg
     8341 tcaggttcag tttaaatggt ccttcgatca ggtgacagga acggtaagta acaccggcaa
     8401 cacgtggttt aagctactga ttaaaccagg atgtgattcg accgaagagg aaggcgatgc
     8461 ctggtatcta cgtccgggag acgtggttca tcagcctgag ttacgtcagc cggggaatca
     8521 ttatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc
     8581 ttcggcggac taagacttat ccatcggacg agggaagaat tccacctcag agactcaaaa
     8641 atcgtttaaa tgatgtggaa gcgatcgtca atgtcaccga tgagctttct catatgcaca
     8701 ccacagtgtt caggagacac ggaaaggtac caggcggcct gctaacgagt ggtttcttca
     8761 ctaattagga cgtaaaatcg gtaacggctg gaaatcattc aatactcaca ctatcgaaag
     8821 tacaccagcc aaccgcgata cgtccctgca tacgacgtac cgcagttatc tttacgattt
     8881 atagctgtac tggtgaatta tgagcaattt gaatccatgc atgacgtgtg gtgcctgttg
     8941 tgcatttttc cgcgtctctt tttactgggc cgaagccgac gatgctggcg gtaccattcc
     9001 cgccaggctc actgaacaaa tatccccttt tcaccgatgc atgagcggta ctaatcagaa
     9061 aaatccccga tgtattgccc ttgcaggaac accgggcaaa aatgcctgct gcacgatata
     9121 tgaaaatcga tcgtccacat gcagagaatt cgccatgtct ggtgagaacg gagaagtcaa
     9181 tgaggcttgc aatcgtgcaa gagctaaata cgggctaaca ccgttataaa tatacaataa
     9241 ttaattgcat tgccccgcca gctgtaatag cggggcttcg tttttcaggg acaacaaaac
     9301 ctgaaacttc ttccattttc cagcaacaat gcctcttctt ctgtagcgtc ctccggatct
     9361 acatcatcct gaagtagcgc gatatgatca ctgactgtcc gcatcagaat atgaaaagca
     9421 acgtttttaa cctataaaaa tggcgctata tttgcgccat ttttatcatt caaagcatta
     9481 tctatttgag cctaaaggga tctcaggatc tggctcatgg gtaattctgt ttcgtaaatc
     9541 tctgcgaata atttcaatag accagaacca gactaaatgt ccaaaaattt cagagacatt
     9601 ctcataccac ggaagatcaa agagaggtgg cgtaagtccc atgagaggga atgaaatcat
     9661 atgaacaaaa agttgcgcta aagcacctgc cagtaagccc tgccaaagtt taattttcgg
     9721 aaatacttcg gcgaccacac agtaaccaac ggcaaaaact atcgaaaaga ttatgtgcgt
     9781 tacaccaacc cagttaaaga catgcccggc gaaggtataa acagccgaat ttggatctgt
     9841 cagtcccaac caatcccgaa gaaaaatgta cggaggattg agaaagttac gggagcaatc
     9901 aatttggcct gcagccctga ttaatgattc cggaccacac gctgcattaa acatatcaac
     9961 cggactacgt ggtggcaatg gaacttctgc cccccactta acaaatgcgg aaacaacgcc
    10021 agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggtttggtg gagtttgttc
    10081 aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttt aggagtattc
    10141 ataaaacaag gcaaatgtaa agaactgtat tgttttgtat aaccacatgg ttacctaatc
    10201 gccaattaat ataagctcca ttatttctcc atatttttat gttagaagtg acaaagagaa
    10261 atgtaatgtt tctctcagag aatataaatc attccttttg cccaccttaa tggtgggctt
    10321 ttttatgcaa aacctgaacg tcctgtggta ttcctctggc ataatgattt tcgcgattaa
    10381 ccaacaacca gaccagcaaa gacgccacca caagcacagt aacatccatc aggcttgcca
    10441 caccccatgc gtgcggctca aagccgccat ggcatattaa tccgcttgcc attttcgaac
    10501 agctgattgg tttggtccgg tgtttccctg cttgcactta aaaatgagcg gataggaaac
    10561 tctcgaaatg gttcgtcgat atgctcacct cgcacctaat caccttaccg aacacgcacg
    10621 gtaaatagat tcgatcctga acccatcggt cccaaatctg tcccaatcaa aaaataagga
    10681 aggtattaat gatgtgtaac ttattgattt aagtggtgcc gataatagga
//
LOCUS       DAPQZD010000163        10703 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPQZD010000163 DAPQZD010000000
VERSION     DAPQZD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.165"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            19..927
                     /locus_tag="RO454_004057"
     CDS             19..927
                     /locus_tag="RO454_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDY0935807.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1134..3125)
                     /gene="tkt"
                     /locus_tag="RO454_004058"
     CDS             complement(1134..3125)
                     /gene="tkt"
                     /locus_tag="RO454_004058"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDY0935808.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3403..4161
                     /gene="loiP"
                     /locus_tag="RO454_004059"
                     /gene_synonym="yggG"
     CDS             3403..4161
                     /gene="loiP"
                     /locus_tag="RO454_004059"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDY0935809.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4591..5511)
                     /gene="speB"
                     /locus_tag="RO454_004060"
     CDS             complement(4591..5511)
                     /gene="speB"
                     /locus_tag="RO454_004060"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDY0935810.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5647..6378)
                     /locus_tag="RO454_004061"
     CDS             complement(5647..6378)
                     /locus_tag="RO454_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935811.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVHNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6524..8500)
                     /gene="speA"
                     /locus_tag="RO454_004062"
     CDS             complement(6524..8500)
                     /gene="speA"
                     /locus_tag="RO454_004062"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDY0935812.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8509..8640)
                     /gene="yqgB"
                     /locus_tag="RO454_004063"
     CDS             complement(8509..8640)
                     /gene="yqgB"
                     /locus_tag="RO454_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDY0935813.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8776..8991
                     /locus_tag="RO454_004064"
     CDS             8776..8991
                     /locus_tag="RO454_004064"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935814.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            9295..10449
                     /gene="metK"
                     /locus_tag="RO454_004065"
     CDS             9295..10449
                     /gene="metK"
                     /locus_tag="RO454_004065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDY0935815.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2644 a         2859 c         2728 g         2472 t
ORIGIN      
        1 tactcaagga agattcatat gaaagcacct gtttcaataa cctcatttcg cccacaaaaa
       61 tctattgcta tcgccattgg cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac
      121 accgttaatg aaggtgaacg cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc
      181 gagccggggc tgggttttaa gatccctttt atggaaagtg tagagaaaat ttccacgcgt
      241 aatcaggctg tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa
      301 atgacggtat cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc
      361 tacaacacca ttgaagcatt aaaagatcgt ctgattgtcc gtcagttgcc aactcaactg
      421 gagaacgttt tcggccaata taccgccatc agcgcggtac aggatcgtac aaaactggtt
      481 caggatctgc aaaatgcaat gcgtaaagcc gttgttggcc cggtggtgat tgatggtgtc
      541 cagattgaaa atattgattt ttctgacgct tatgaaaagt cgattgaaga ccgaatgaaa
      601 gcagaagtcg ccattgccac acgtaagcaa aatctggaaa cagagaaaat ccaagcgcaa
      661 atagcagtaa ctcaggccca ggcagaagca gacagtaaac tggcggcagc caaagcagaa
      721 gctgagacca ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg
      781 gctgaagcag aagctatccg cctgcgcggt gaagctctgc gggagaatcc cggacttgtt
      841 gccctcacca ctgctgagcg ttgggacggt aaactgcccg ataccatgat ccccggcagc
      901 acagttccgt ttatctctac taagtaaaaa aaggccgctt tcgcggccct tgagactgat
      961 agcaaagaca aaactgcctg atgcgctacg cttatcaggc tgcaatatgt tgaatttgca
     1021 aaatcttgta ggacggataa ggcgttcacg ccgcatccgg cataaacaac gcgcactttg
     1081 ccaacaatct gaagggccgc atttgcggcc cttctcacaa agcatcttac cgattacagc
     1141 agcgcttttg ctttcgcaac aacgttgtca acggtgaagc cgaactcttc aaacagctgc
     1201 tctgccggag cagattcacc gaaggtggtc atgccgacga tagcaccgtt caggccaaca
     1261 tacttgtacc agtagtcagc aatgccagct tccaccgcaa cacgagcggt aaccgctttc
     1321 ggcagtacgg attcacggta agccgcgtcc tgcttgtcga atgcgtcggt agacggcatg
     1381 gaaaccacgc gcgctttcac gccttcggca gtcaattttt cgtatgccgc tacagccagt
     1441 tcaacttcag aaccggtcgc gatgaagatc agttccggct gaccagcaca gtctttcagc
     1501 acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg ctgcgccagg
     1561 ttctgacggg agaggataag cgcagtcggg ccgtcctgac gttcaacgcc gtatttccac
     1621 gctaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc
     1681 agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag accgatggag
     1741 tcgtgggtgt aaaccatcac ctgacgctgt ttcatcagcg cagccatacg tacggcgtta
     1801 cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaaacc accgtgcagg
     1861 gagataccgt tggcaatcgc agtcataccg aattcacgta caccgtagtg gatgtagtta
     1921 cccgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt agacggcgcc
     1981 aggtctgcag aaccgccgag gaattccggc aacagcggcc cgaacgcttc gatagcattc
     2041 tgagacgctt tacggctggc gatcttcgcc gggttagcct gcagtttagc aatgaactct
     2101 ttcgctttcg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt aaattcagcg
     2161 gcttcctgcg gataagcttt cgcgtaagca gcgaatttct cgttccatgc ggactctttc
     2221 gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat ttcgaacggc
     2281 gcgtatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca
     2341 ccgtgggagt cgtgggtacc ggctttgttc ggggaaccga aaccgatgat ggttttgcac
     2401 atcagcaggg acggtttgtc agtcactgcg cgcgcttctt ctactgcgcg tttgatggat
     2461 gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt
     2521 gcggtgtcgt cggtaaacca gccttcaacg tgaccatcga tagaaatacc gttgtcatcg
     2581 tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac ttcgtgggag
     2641 atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc gacgatgtcg
     2701 tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat accgactgcg
     2761 ttggcaatac cctgacccag cggaccggtg gtggtttcta cacctgcggt gtaacccact
     2821 tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc ttccatcggc
     2881 agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg gccgttggac
     2941 agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt caggaaatca
     3001 cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg accggatttg
     3061 gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc tttacgtgag
     3121 gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg acgacagcgc
     3181 gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg tcatgtacat
     3241 ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg cgcaatctac
     3301 tcgccggtcc actgcacttt tccttatact tcgattgagc gtcaattcac ctgcagatgg
     3361 cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta
     3421 ttggtagcaa tgagcgtggc aacggtactg actggttgcc agaatatgga ctccagtgga
     3481 ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa
     3541 accctgagcg atcaggcatg tcaggatatg gacagcaagg cgacgattgc gccagccaat
     3601 agcgaatacg ctaaacgtct gacgacaatt tctcgcgcgt taggcgataa catcaatggt
     3661 cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc aatggctaac
     3721 ggctgtatcc gcgtctatag cgggctgatg gatatgatga cggataacga agtcgaagcg
     3781 gtgctcggtc acgaaatggg gcacgtggca ttaggccatg tgaagaaagg aatgcaggta
     3841 gcacttggta caaacgcaat tcgcgttgcg gcggcctctg caggcggaat tgtcggcagt
     3901 ttgtcacaat cacaactagg agacctgggc gaaaagctag tcaattcgca attctcccag
     3961 cgccaggaag cagaggccga tgattactcc tacgatttgc tgcgccaacg cggcatcagt
     4021 ccggctggcc ttgccaccag ctttgaaaaa ctggctaaac tggaagaagg tcgccaaagt
     4081 tcaatgtttg acgaccaccc agcatccgcc gaacgcgccc aacatattcg cgatcgtatg
     4141 agcgcggatg gagttaagta aggcatggtg gtgccaaacg taggtcagat aaggcgttta
     4201 cgccgcatct gacatgaatg gcacatttgt tatcctgtgc gcgttgccgg atgcgatgct
     4261 ggcgcatctt atccagccta cgggtgccga acgtaggtcg gataaggcgc tcacgccgca
     4321 tccgacatta atggcacatt tgttatcctg tgcgcgttgc cggatgcgat gctgacgcat
     4381 cttatccggc ctacgggtgc cgaacgtagg tcggataagg cgctcacgcc gcatccgaca
     4441 ttaatggcac atttgttatc ctgtgcgcgt tgccggatgc gatgctgacg catcttatcc
     4501 ggcctacggg tgccgagtgt aggtcggata aggcgttcac gccgcatccg acattaatga
     4561 cacatcaccc gtgcgcctct tatctggcgc ttactcgccc tttttcgccg cctgaatata
     4621 cagcatctcc agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc
     4681 cggagccact tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtaccag
     4741 tttaatggcg cgatcggagg tcaggccgcc aattaccggc gtaccggtgc ctggtgcaaa
     4801 agcaggatcc aggcagtcga tatcaaaggt caggtaaacc ggcatatcac ccacaatctg
     4861 tttcacctgg gcgataatgt catccacgct gcgatcgttc acctggcagg cgtccagcac
     4921 ggtaaagccg ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg
     4981 gtcgatcaga ccttctttcg gcgcggtata gaacatggtg ccgtggtcaa attcacaacc
     5041 gttcgcatag gtatcggtgt gggcgtcaaa gtgtaccaac gccattttgc cgaaatgctt
     5101 cgcatgagcg cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg agagcatacg
     5161 cttaccggca gccagcagct tctcggcgtg cgcctgcagc ttctcgctca tctcacgggc
     5221 atcgccgaag gcatatacca gatcaccgca gtccacgacg ttcaggcgct cacgcatgtc
     5281 gaaattccac gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc
     5341 tgccggaccg tgacgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt
     5401 aatcacccag tcagcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc
     5461 aaaggcgttg gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact
     5521 ccttttacaa agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt
     5581 tggatttgcg aaacacaagt ttgtgctaat tatcgcctta aatgggcgag gcatcaagcg
     5641 aggttgctat ttccgacacg gtggtggcgc atagccattg ttatctgccg gaaacgcttg
     5701 tccagagcac ggcggcagcg cgttttgcgc cgggccttgt aattcaaagg cggcaaagcc
     5761 gattacaccg gtattatgca catcgccttg cgccgaattg gctgcgtagg aatcagcaac
     5821 gttggcgaac tgaaaagccg cttcggtatg tttatctttg cggaatcctt taatcgctaa
     5881 cgaatctccg gcattcacaa tataaccgtt gttattcagc gaaccttgct tgccgttcag
     5941 cacatccagg ccatcaaccg tagcaacgat ctcgtagttg gtgttacgac tgtaattacg
     6001 gatatataat tgataattca ttccgattct ggcatggaca atatagcgtg gttcattatt
     6061 ggatgaatca taaacccgcg taataggtag agaattaaaa ttaccatcgc gaatagcata
     6121 ttccagttcg ttaatacgaa tactataaac gcggtcgtag ccggaaggaa cattagtcga
     6181 ataactaata acgatcatct ctgccggatc gcgccaggct cgttcagcag atacgccctg
     6241 aaccgttgag tgcacatccc caccccaggc ggtgccgaca ttacccgatg cactgctatt
     6301 atactgcgca ttactggaac aaccggccag cagcacggct aatgctatca acgcgctacg
     6361 taatttccat tttttcataa ccgttccctc tgcatttaat taatgcagcg attgtaggga
     6421 gattatttaa taagaaaagc agctaaatta tttttaagaa tatataaaaa gaaagcgggc
     6481 cgatttacgt aaataaatcg ccccgccagc ggaataatct taattattca tcttcaagat
     6541 aggtataacc gtacaaacct gcctcgaact cttcaaggaa ctgttgttgc agttcagcat
     6601 caagatcggt tttcttcacc tgatcgcgga actgagttaa cagcgttttc ggatcgagct
     6661 gcacatattg cagcatgtcc gccacggtat cgccttcgtc agacagttct acctctacgc
     6721 taccatcagg gaagacgaac acgtcaaccg cttcggtatc accgaacagg ttgtgcatgt
     6781 tgccaaggat ctcctgatat gcgccgacca taaagaaacc gagcatcggc ggattctccg
     6841 gatcgtactc cggcataggc attgtcgtgg caataccgtc accatcaata tagtggtcga
     6901 tagcaccgtc tgagtcacag gtaatatcca acagcacagc gcggcgttcc ggcacttgat
     6961 ccagcccttc cagcggcaga accgggaaca actggtcgat cccccacgca tccggcatcg
     7021 actggaacag cgagaagttg acgtacattt tgtccgccat acgttcctgc agctcgtcga
     7081 taatcggacg atgagcacgg ttttgcggat ccagctgctt ttgcacttca tggcacatgc
     7141 tcaaataaag ctgctcagcc catgcgcgtt cttgcaggct aaaggtgccg gaagagtagc
     7201 cgatatgaat atcgtgcaga tccatctggc tgtcgtgtaa ccattcacgc agagaacggc
     7261 gagttcccgg ttcgtgcatt tcctgccagg tttcccacat gctttgcagg gcgcgcggtg
     7321 cgtcttctgc aggcgcggtc ggcaccgtgt attcgttacg ttccacgccg atgatattcg
     7381 acaccagcac ggtgtgatgc gcagtcaccg cacgacccga ttcggtgatt accgtcggat
     7441 gtggcagacc attttcttca cacgcatcac caatcgccca gatgatgttg ttggcgtatt
     7501 cattgaggcc gtagttcacc gaacagtcgg actgcgaacg ggtgccttca tagtccacgc
     7561 ccagaccgcc gccgacgtcg aagcactgaa tattgacgcc cagcttgtgc agctcaacat
     7621 agaaacgcgc ggattcacga acgcctgtcg cgatatcgcg aatattcgcc atctgcgaac
     7681 cgaggtggaa gtgcagcagt tgcaggctgt cgagacgccc ggcttcacgc agggtttcaa
     7741 ccagttgcag tacctgagtc gccgccaggc cgaacttcga tttttccccg ccggaggact
     7801 gccatttacc ggagccctgc gaagccagac gtgcacgcac gcccagacga ggaacgacat
     7861 tcagacgttc tgcttcatcc agcacaatgg caatttctga catcttctca atgaccagat
     7921 agaccttgtg gcccatcttc tcgccaatta atgccagacg gatatattcg cggtccttat
     7981 aaccgttgca gacgatgacg ctacgggtca tgccagcatg tgccagcacc gccatcaact
     8041 cagctttgga accggcttcc aggcccagcg gttcgcccga atgaatcagg gactcaatca
     8101 cgcggcggtg ctggttaact ttgatcggat aaacgaggaa gtaatcgccg ttatagccgt
     8161 aggattcccg cgcacgtttg aacgcggcgt taatagaacg caaacggtgc tgcaggatct
     8221 gcgggaaaca gaacagtgca ggcagacgct ggccctgtgc ttcacgagtt ttcactaact
     8281 gcgcgagatc gacgcgagct tccgggacgt ccgggtccgg gcacacgctg atgtggccca
     8341 gctcgttaac gtcatagtag ttattgcccc accaggcaat attgtaagta cgcagcatct
     8401 tgctggcttc ctgggagctc attgcaacct cctgcatgga gcgtagtaca ccgtgttcgc
     8461 ccgctgacga aggcaaaccc atagacatgt cgtcagacat agcgaacctc aaattatttt
     8521 attaagtgta aaacagttaa cgactatcgc agcctggaac tgcgataaca acccataagc
     8581 acatggattt tccagcagtg aatgctgacg ctccaactgc gcaaccggtt tctttttcat
     8641 aacattatta agcacataac cgaacgtaag tgtgacagtt cggcgaaacc acgagaaaac
     8701 tcttgttttt acaagagcgc ccttgttcag tcctcagtag ctgtaaccag ctcttgaatc
     8761 ctgagaagcg ccgagatggg tataacatcg gcaggtatgc aaagcagaga tgcagagtgc
     8821 ggggaacgaa tcttcaccag aacggtgaga caggttaagc agcagacaac ggttcattat
     8881 ttcgtatcac ctccacggcc gcctgttaag acgaacccac aagccaaaac tctgttttca
     8941 acccggctgg aagtggcaac acgaaagaaa cgtcgtgtgc tttttattta agccgcgcgc
     9001 cgcgttttat accccacaat ggcagaaaat tgcaaaagat aaatacgcag aatgccagca
     9061 ttgtcaggaa aaatttccag ccacgttttt aacagaatga gacacgattc aaaaaaaagt
     9121 ggaaattggg tgaagaactg acctaaaata gccatccaga tgttaatcca tccataccga
     9181 ttaacactca gactgccagt gtttttaacc tgcagagtcg tggtaggatc cgctaccaca
     9241 gaaaatccac acaacagttt gagctaacca aattctcttt aggtgatatt aaatatggca
     9301 aaacaccttt ttacgtccga gtccgtctct gaagggcatc ctgacaaaat tgctgaccaa
     9361 atctctgatg ccgttttaga cgcgatcctc gaacaggatc cgaaagcacg cgttgcttgc
     9421 gaaacctacg taaaaaccgg catggtttta gttggcggcg aaatcaccac cagcgcctgg
     9481 gtagacatcg aagagatcac ccgtaacacc gttcgcgaaa ttggctatgt acattccgac
     9541 atgggctttg acgctaactc ctgtgcggtt ctgagcgcca tcggcaaaca gtctcctgac
     9601 atcaaccagg gcgttgaccg tgccgatccg ctggaacagg gcgcgggtga ccagggtctg
     9661 atgtttggct acgcaaccaa tgaaaccgac gtgctgatgc cagcgcctat cacctatgca
     9721 caccgtctgg tacagcgtca ggctgaagtg cgtaaaaacg gcactctgcc gtggctgcgc
     9781 ccggacgcga aaagccaggt gactttccag tatgacgacg gcaaaatcgt tggtatcgat
     9841 gctgtcgtgc tttccactca gcactctgaa gaaatcgacc agaaatcgct gcaagaagcg
     9901 gtaatggaag agatcatcaa gccgattctg cccgctgaat ggctgacttc tgccaccaaa
     9961 ttcttcatca acccgaccgg tcgttttgtt atcggtggcc cgatgggtga ctgcggtctg
    10021 actggtcgta aaatcatcgt tgatacctac ggcggcatgg cacgtcacgg tggtggtgca
    10081 ttctctggta aagatccatc aaaagtggac cgttccgcag cgtacgcagc acgttatgtc
    10141 gcgaaaaaca tcgttgctgc tggcctggcc gatcgttgtg aaattcaggt ttcctacgca
    10201 atcggcgtgg ctgaaccgac ctctatcatg gtggaaactt tcggtaccga gaaagtgcct
    10261 tctgaacaac tgactctgct ggtacgtgag ttctttgacc tgcgtccata cggtctgatt
    10321 cagatgctgg atctgctgca cccaatctac aaagaaaccg cagcatacgg tcactttggt
    10381 cgcgaacatt tcccgtggga aaaaaccgac aaagcgcagc tgctgcgcga tgctgccggt
    10441 ctgaagtaat ttttcttcac ctgcttttaa aggccagcct cgcgctggcc tttttctttt
    10501 acctcaccgc ggtggatagg cgttcatgcc gcatccggca aaaagctgcc cgcacaataa
    10561 catcattctt cctgaacacg ttttaccgca aattatcatc gctactgaaa ccgattacac
    10621 caaccacgcc agccaaatat ttgtaatatt ttcatattat tattcagctt tcacagttgt
    10681 tacatttctt ttcagtaaag tct
//
LOCUS       DAPQZD010000164        10685 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPQZD010000164 DAPQZD010000000
VERSION     DAPQZD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.166"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..319)
                     /locus_tag="RO454_004066"
     CDS             complement(<1..319)
                     /locus_tag="RO454_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDY0935816.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(618..956)
                     /gene="osmE"
                     /locus_tag="RO454_004067"
     CDS             complement(618..956)
                     /gene="osmE"
                     /locus_tag="RO454_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDY0935817.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1158..1985
                     /gene="nadE"
                     /locus_tag="RO454_004068"
     CDS             1158..1985
                     /gene="nadE"
                     /locus_tag="RO454_004068"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDY0935818.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2215..3102
                     /gene="cho"
                     /locus_tag="RO454_004069"
     CDS             2215..3102
                     /gene="cho"
                     /locus_tag="RO454_004069"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDY0935819.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(3062..3637)
                     /gene="ves"
                     /locus_tag="RO454_004070"
     CDS             complement(3062..3637)
                     /gene="ves"
                     /locus_tag="RO454_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDY0935820.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3840..4325)
                     /gene="spy"
                     /locus_tag="RO454_004071"
     CDS             complement(3840..4325)
                     /gene="spy"
                     /locus_tag="RO454_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDY0935821.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4655..5623)
                     /gene="astE"
                     /locus_tag="RO454_004072"
     CDS             complement(4655..5623)
                     /gene="astE"
                     /locus_tag="RO454_004072"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDY0935822.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5616..6959)
                     /gene="astB"
                     /locus_tag="RO454_004073"
     CDS             complement(5616..6959)
                     /gene="astB"
                     /locus_tag="RO454_004073"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDY0935823.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(6956..8434)
                     /gene="astD"
                     /locus_tag="RO454_004074"
     CDS             complement(6956..8434)
                     /gene="astD"
                     /locus_tag="RO454_004074"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDY0935824.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8431..9465)
                     /gene="astA"
                     /locus_tag="RO454_004075"
     CDS             complement(8431..9465)
                     /gene="astA"
                     /locus_tag="RO454_004075"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDY0935825.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9462..10682)
                     /gene="astC"
                     /locus_tag="RO454_004076"
     CDS             complement(9462..10682)
                     /gene="astC"
                     /locus_tag="RO454_004076"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDY0935826.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
BASE COUNT         2571 a         2951 c         2633 g         2530 t
ORIGIN      
        1 aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga
       61 ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga
      121 tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt
      181 tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg
      241 cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat
      301 aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc
      361 cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt
      421 gtggctgaaa ggagtatacg gtataggcct gagcaatagt atttctttga cctgataaat
      481 tgtctctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag
      541 ataattaaga acacaggcca aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa
      601 gctttcaggc gaagtgatta cttcgtagcc tgcggatcag tgtcgtattc agcacaggtc
      661 tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct
      721 ttaccatcac gttgacccag gatgtaggtc tggcaagtac cacgagcatg gatcatgctc
      781 acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg
      841 acgtctttca ccacaggctg tacaaactgg tctttggtac gatcataagc cgtacaaccc
      901 gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc
      961 cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc
     1021 ccgtgaacga aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca
     1081 gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt
     1141 ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac
     1201 cgcagattaa tgctgaagag gaaattcgtc gtagtatcga ttttctgaaa agctacctgc
     1261 aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc
     1321 ttgccggtaa gctgtgccag atggcgatta atgagctgcg ccaggaaacc ggcaacgaat
     1381 cactgcaatt tattgccata cgcctgccct atggtgtcca ggctgacgaa caagattgcc
     1441 aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg
     1501 tactcgccag cgaacaggcg ctgcgcgaag cgggcattga actgagcgat tttgtccgtg
     1561 gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagtg
     1621 gtgtcgtggt gggcaccgat catgcagcag aagctattac cggattcttc actaaatatg
     1681 gtgacggcgg tacggacatt aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt
     1741 tactggcggc attaggttgc ccggaacacc tttataagaa agcgccaacg gccgatcttg
     1801 aagatgatcg cccttctcta ccagatgaag tggcactcgg cgtgacctat gacaatatcg
     1861 acgactatct ggaagggaaa aacgtacctg aacaggtcgc cagaacaata gagaactggt
     1921 atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa
     1981 agtaataatt tgcacctatt ggattgtgcg aaaaagagta atttgttcac gccggatgcg
     2041 gcgtgaacgc cttactcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa
     2101 taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt
     2161 ttaatcgctc cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta
     2221 cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa
     2281 catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc
     2341 gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt
     2401 tctcatttac gcaccccgga tgaagccgcc atgctgcgac aatcccgacg catcagctgg
     2461 atatgtaccg ccggtgaaat cggcgctcta ctccttgaag cgcgattaat caaagagcag
     2521 cagccgctgt ttaataaacg cttgcgccgc aatcgccagc tctgcgccct gcaattaaat
     2581 gaaaagcgtg ttgatgtggt gtatgccaaa gaggtggatt tttcacgagc ctccaacctg
     2641 tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagaccat cgccgatgaa
     2701 caaaaacttt gttatggcct gttgggactg gaaccgttaa gccgcggtcg tgcatgtttt
     2761 cgttctgcat taaaacgttg cgccggggcg tgttgcggta aagagagcca tgaggaacat
     2821 gcgctacgct tgcgccaggc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggc
     2881 gcggtggcat tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg
     2941 ctgtggctgg gggcggttaa ttcgctgaaa gaagcgacaa cgttaattcg ggcacccgcc
     3001 gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa
     3061 attactgaac ttgatccggt gaatgaccag caagccagtt gatttcactg aacaacagtt
     3121 taccttgcgg ttgcaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt
     3181 cggtagtgag cagtttgtcg cccaattgcc acgccccgtt tattacaaac accaccccgc
     3241 cgcgtgaacc aaaggtggta aacgtacgtt cggcaattct gactttcgcc ttacagacat
     3301 ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt ttcgctttta
     3361 ccaccagatc cgctgcaaag gaaaaaggtt gtaacggttt taaggtatgg ttaaagcggt
     3421 ctgcgctttc aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccataccgg
     3481 gaaacaaaga aaactcccca ttcgccgcga tggatgtaat gctggcacgc cagtaaaaat
     3541 cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca
     3601 gattcaccga cattttacgc atatcaaagt attccatacc cgctcccggc ccgttctttt
     3661 cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt
     3721 ggcgatcagg ctttttttgc atctgtttga gaagagaaaa gaaaaccgcc gatcctgtcc
     3781 accgcattac tgcaaggtag tggacaagac cggcggtctt acgttttttg gctgaaagat
     3841 tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca
     3901 aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat cttgttctgg
     3961 gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct
     4021 tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct
     4081 tccagtggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg gatctgctgt
     4141 ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctcatgcgg accgaacttg
     4201 cctttatggt gcatcatcgg cttcgcgtca gccggtgctg cagtagtggt gtctgcggca
     4261 tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta
     4321 cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt
     4381 gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat
     4441 gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag
     4501 tgatgagaga atattacaac acgatgattt tgcagagatt atgaagaact ataccggatg
     4561 actggtgata aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac
     4621 ttaatacccg cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc
     4681 ccgcacgtaa tcccaacgct accaacggat tagggaataa cacatactct acatcatggg
     4741 ttacggtaaa acgttcctcc ccgtcctgcg ccagcaatgt tcctttctca aacggcataa
     4801 aattcagcgt gtcatttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg
     4861 aaaccacccg ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca
     4921 gcgcagcaat tgcgctggca gttactgcaa actggcgaag atcgttttgt ccaaagggca
     4981 acgctttgcc gagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg
     5041 taaacgtacc accaggctcc tgatggaaca ccagtgcctc cagacccgcc gcacccagcc
     5101 acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aaccgcggat
     5161 gcaaggagcc acgaattgcg gtatggagat caaggtgcca gcgcacagat tctttgccct
     5221 ggtcataaaa atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tccccgcttt
     5281 cagcaaatag ctgccaacga ccgccaaaca ttcggttcat atcgctatgg caataacgtt
     5341 tcccttgctt cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga
     5401 tctcgccgtg agatatcgcg ccaagcaacg catccagcat ttccacaggt gccgtctcat
     5461 taccgtgtat tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat ggcgtcagtt
     5521 ccagcacgcc atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa
     5581 ccggtttttt acccgttaag gtcagagcaa gaaaattatc catttccccc tccctcgcgc
     5641 tggaatggat aaaccgaacc aagatccaat aattgagtca gtgtatccag cgcttcccgt
     5701 ccttcgcgca gcaattgtgg atcggcaaga tccgccgcag taagacgatc gcggtagtaa
     5761 cgatccaccc agtcattgag cgcattaaac agcgtatcgt tcatcatcac cgccggattc
     5821 accgcccggc gttcttcttc tgtcaacacc acgcgcagcc gcaggcacgc cgggccaccg
     5881 ccattcgcca tgctttcacg taaatcaaaa acttttagtg agcttattgg gttatcggca
     5941 gcaagcaatt cattgagata acaccatact ccagcatgtt cccgacattc ctgaggcagc
     6001 accaacatca tggaaccatc atcgcggctc agcagttggc tgttaaacag ataagtagac
     6061 accgcatctg acacggaaac ctgcgccgtg ggcacctcta ttgccataaa accactgacc
     6121 cgtgcacgca gatttgccag taactgtgac tggcgagcga acgcctgttg atggcaaaac
     6181 agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgccctg gtcgataacg
     6241 tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattcaa ccttgccacc
     6301 gcctcgctgg cttcacgggt ctgccgcgcc ggataacggg aaggccgggt atcattgccc
     6361 tcttctcgcc cgtagacaaa aagttgcata cccggttcac cgtaatgacc gccgagacga
     6421 ttgtggtttg ccgccccctc atcaccgagc aaggctacct gcggcaacgc cgaatggacg
     6481 ctaaatttct tttcgtcgtt aaaaatcgct tttaacagcg attcggtgac gggggcttcc
     6541 agcgaacggt gaaatttatt gttcaggtta gcaacagtga gatgcacttt gccatccagc
     6601 gtatcggcgg atggcgcgat cgttgccgca ttggctaccc acattggcga agcggaactg
     6661 acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg ctcatcgcta
     6721 ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg cgggatcacg
     6781 gcctggggga atcccgcatc ggcaagtttt ttcattttca gtaagccctg ttttgccgcc
     6841 agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc aaacgacagg
     6901 cccgcgtaat ggtgcgtcag ccctaccagc ccgtcgaaat tgacttccca ggcgttcatc
     6961 gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaac gttaacgagt
     7021 ccgactccag acttgccatc ggccaggcac aataatctgc ggcataccag gcgctggggc
     7081 gatgattgcc ggaagcgcca atgccgccga atggcgcggt actggcagca ccggtaagcg
     7141 gtttgttcca gttaacaatc cccgcccgtg cctccagcaa cagttgatcg aatttttccc
     7201 gctcggggga aaccagaccg caagagaggc cgaagcgagt gttattcgcc attcgaatcg
     7261 cttcatcgaa attgtcataa cgccagacgc gcaataacgg cccaaacact tcttcatccg
     7321 gcacgccagt aacgcctgtc atttcaatga tccccggcgt cagcaacgat gtccctgctt
     7381 gtaataagcg cggcgcaagc aggggccgtc cgcccatcgc ctccagttcc tgccaggcag
     7441 taaccacctg ctgtgcggcc tgctcagaaa tcagcccgcc aataaacggc tgcggttcgt
     7501 catcccaagt gcccggcgtt aaccgctggc tgacggcaac caggcgagca agaaatgcat
     7561 cgccctgcgt tccacttttc agcaataaac ggcgggcgca ggtgcagcgt tgcccggcag
     7621 tgacaaacgc cgactgaatg gtcagatgga cagcggcatc gatatccgcc acctcatcga
     7681 taattagcgg gttattaccg cccatctcaa gggcgagaat tttctccggc tgaccggaaa
     7741 gctggcgatg cagctggtag ccggtattgg cgctgccggt aaacagcaaa ccgtcgagat
     7801 cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg ttcagcacgc
     7861 ccggcagcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc cacggtgtca
     7921 gttcgctggg tttaaaaata atggtgttac ctgccagcaa tgccggaaca atatgtccgt
     7981 tcggcagatg accagggaaa ttatacggtc caaacactgc cagtacgccg tgcggacgat
     8041 ggcgcaagct cgccgcgccg tccggcattt cgctacgctg ctcaccggta cgaacgtgat
     8101 acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt gccgcttccc
     8161 agcgcggctt gccggtttct ctggcaataa tcgcggttaa ttcagcttta ttgctttcca
     8221 gcaatccggc aaagcgttca actcttacct gacgatcgct aaatgagagc ctcgcccagc
     8281 gcggaaacgc cgctcgggca gcccgacaag cctgctcgac ctgagcagca tcggcatcat
     8341 tgccctgcca taacacctcg cccgataccg gattacgctt cacacgcaat gcgccctggc
     8401 ccgttaccca gtcaccgtta atccataaag tcatgctgtt ttctcctctg cgcacaggcg
     8461 caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg cggcggttaa
     8521 aatcaaacgc tcggtcgctg gatcggtacg caccagcacc acgcggaaat ggtgataatt
     8581 ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt cggccacttc
     8641 caccagccga cttttacgga tggcgcgcac gcggtcgata tcacactcaa gtgtcggccc
     8701 gccgtcaaag atgtcgatat agttacggta gcgaaaacct tctttctcca gcaccgcacg
     8761 ggcaggtgcg gtttgcggat gtacctgacc gatgacgtcc tgtgcttcct gggataaaaa
     8821 gtgcgtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt gcccggtgcc
     8881 gcagagaaaa tcagcgcggc taaaatccat cgaaaagaag cgtttaccga ggctttgcca
     8941 gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcggcgacca ctttgtcatt
     9001 aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca aatagccgtt
     9061 gccctctttg cgccactccg ggtcgagaaa cagcgtgcac agctcgctgc tgccggtgtg
     9121 atcgttactg agaaacagcg tcggcaatgc gttatagaca ttcagctctt tcgaagcgtg
     9181 aaccagcgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg ccacctcaat
     9241 ggcgcaaatc cccgccacag tgcctgactc gctatcttcc agtacaaaca cataaccctg
     9301 ctcacttttg ggcagttcgc cttgccaggt tttgattgcc ctttcaatac gcaccgaaag
     9361 cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg caagctgcat
     9421 cagcgccgag acatctgagc gctcaacggg acgaatgacc atcatgatga acctcggcta
     9481 acaaagtgtt cgcaagcagc tgcaaagcgc tccagtccgg tcgtcacctc ttcttcgctg
     9541 acattgagcg ctggcgcaaa acgcaccacg ttgccacccg caatcagcac catcacgcct
     9601 gctttcgccg cttcctgaga gagctgtttc gcttgcccgg cgtaatcggc attcagtaca
     9661 cagccaatca aaagacctaa gccgcgaact tcactgaaca aactgtagtg gtgattaatg
     9721 gaatttagac gctcaacaaa ccagtcgtga cgctgtttaa ccccgttaag catctctggc
     9781 gtgttgatga gatccagcac tttgcctgcc accgccgagg ccagcggatt gccgccatag
     9841 gtggtgccgt gagtgccaac ggtcatcacg ctggcgcact cttcggttgc caacaacgca
     9901 ccaaccggga aaccgccgcc cagcgctttg gcggtagtta acagatcagg cgtcacgccg
     9961 tagtgcatat aggcatacag ttccccggtg cgcccgacac cggtttgtac ttcatcaaag
    10021 ataagcaacg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa tgcgttgctg
    10081 gctggcacca caccgccttc cccctggatg ggttcgacaa tcaccgcaca ggtggcgtcg
    10141 tcaatcagcg cactggcaga gttaatatcg ttatatgcag catgacgaat atccggcggc
    10201 agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt aaacagcgtg
    10261 cgaccatgaa acgcattttt gaacgccacg atgccgctct tatggctgcc gtagcggtcg
    10321 tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc ggagttacaa
    10381 aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg cagtaccggc
    10441 tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt cagcgcctca
    10501 cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc gaagtcgata
    10561 tactctttcc cctgctgctc ccacaagcgc gaaccttcgc cacgtaccgg tataaacggt
    10621 gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat tggctgagac
    10681 atagc
//
LOCUS       DAPQZD010000165        10641 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPQZD010000165 DAPQZD010000000
VERSION     DAPQZD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.167"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..1349)
                     /locus_tag="RO454_004077"
     CDS             complement(<1..1349)
                     /locus_tag="RO454_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDY0935827.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFTAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRGIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
     gene            complement(1488..1883)
                     /locus_tag="RO454_004078"
     CDS             complement(1488..1883)
                     /locus_tag="RO454_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDY0935828.1"
                     /translation="MKLWPTLGVAFLLITSMRLSWSLGRENARNEAQASTLKSTVDTL
                     NIISAGVQDMQQVLAQLRAENQQRNQDGEVRREQLRNDIAKDECAHALPDARFTDRLR
                     RHAERARASAVSPAYTADADHAGNASPLP"
     gene            complement(1880..2272)
                     /locus_tag="RO454_004079"
     CDS             complement(1880..2272)
                     /locus_tag="RO454_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDY0935829.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGNQVSWEWPLYEKIAAAFRQASQEL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(2269..2592)
                     /locus_tag="RO454_004080"
     CDS             complement(2269..2592)
                     /locus_tag="RO454_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDY0935830.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            complement(2595..2795)
                     /locus_tag="RO454_004081"
     CDS             complement(2595..2795)
                     /locus_tag="RO454_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDY0935831.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(2795..3289)
                     /locus_tag="RO454_004082"
     CDS             complement(2795..3289)
                     /locus_tag="RO454_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDY0935832.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            complement(3391..4191)
                     /locus_tag="RO454_004083"
     CDS             complement(3391..4191)
                     /locus_tag="RO454_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDY0935833.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     IANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(4237..5289)
                     /locus_tag="RO454_004084"
     CDS             complement(4237..5289)
                     /locus_tag="RO454_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDY0935834.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLAVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(5313..6149)
                     /locus_tag="RO454_004085"
     CDS             complement(5313..6149)
                     /locus_tag="RO454_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDY0935835.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            6304..8055
                     /locus_tag="RO454_004086"
     CDS             6304..8055
                     /locus_tag="RO454_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDY0935836.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFMVMEF"
     gene            8055..9101
                     /locus_tag="RO454_004087"
     CDS             8055..9101
                     /locus_tag="RO454_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDY0935837.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(9116..9640)
                     /locus_tag="RO454_004088"
     CDS             complement(9116..9640)
                     /locus_tag="RO454_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935838.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVRLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLEAAEGFRRMPLSQIPAATPKARGSNDERKLTFSIETV
                     VDDTPGEVAFVHTPVSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            10364..>10641
                     /locus_tag="RO454_004089"
     CDS             10364..>10641
                     /locus_tag="RO454_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HDY0935839.1"
                     /translation="MNPITLQIISNAIVLLGVLVAIGTIIYNVRTAKKTQTANFLFES
                     RQDMQYIESLHTLKQVHRSGKSFRSYVFPCDGCIITDEEMAERRKFQY"
BASE COUNT         2291 a         2831 c         2887 g         2632 t
ORIGIN      
        1 tctgacaact gaattccgcc attcttaatg ctccagccct gcgcagcagc atctccggct
       61 gtcggcaaca gggagattgt gcgtacggat gcatcttcag acggccccga tggcgtgtcg
      121 ccgccgggcg agggtttgat ttccggtgcc ttaccactga tgaaggctaa ggtgcgaccg
      181 gctacgttca gaatagcggt tgccatacga tcgggaataa tgccacgacg cgcccatgag
      241 ctgaaatgcg tctggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
      301 taatatccct cacttgcagc atccggatct tccgttggtg cgttggtgga ggtattgttg
      361 ccactaccat cggtcaggaa cggcacaaag aaaacgttgt cgctctccct gtttttgtac
      421 gccccgtaaa tggtgtcgta ctgcgtgccg taggtgtttt tccagtaata cgtcgtgtca
      481 ccacaaatcc atggcacact tgccgctctg cctccatgac actgcgtgtt aaactcggta
      541 atgtccgcac gaaactgttt cagcatggcc gtaaacagcg gaggttgctg tgagtacgta
      601 gcggcgctca tgtcaaattc gccctgcatc cagcatacgg ccagcagcat gttttttggg
      661 tttttctgca atgcggcctt cgtgcgcaaa atcaggtcct gatataaagg tttacccaca
      721 ccccagcgag ccgaatcctg actggctcct gtggactcgc tgaatgtccc ctccgcgccc
      781 tgggtgaatg ccgaaccacc acgacagcat ggaaccagta aaatcccggc attctgtgga
      841 atgtaaggca acaggcgttt ggcaatatgc aagccctgtc cgacacagcc atactgccct
      901 ttgctcaggt ctgcattcgg gtgattcagt gcgctcatat cctgaacatc atgcaggcag
      961 tggtctgccg gaatgatgtc gttaaatgcg catgctttac caccgggagt cactgtgttg
     1021 cgacgggcca gttgcttaat gcgtgggtgg ggcgcatcgt aagaatccgg cagcggcatt
     1081 ccctcaccat atgacatggc attggattga ccagcaagta ccaccacgta ataccactcc
     1141 ggcacgctgc cgtgctgggg ggaatcaggt gagatgtctg tagtgccatc ggccaccatt
     1201 gcctgcatta gataccacgg ggtgccgggt tcgtaggcaa cccgcgtctc cccctgaaga
     1261 tgccaccctt tcgcaagatg ttcattcacc ttttgagtca gttcagcctg tgtcatggct
     1321 gtaatcagtg caaagttctt actggtcatt gtgttactcc cgtctggtgt gtcagttcgg
     1381 ctattctctt atccagttca gcaatggccc gtttatccgc gttacaggtt tccagtgcat
     1441 ccagaaggcg gtcgccccat attccgaggt ttccccatgt gggtgggtca gggaaggggg
     1501 gaggcgttac cggcatggtc agcgtctgcg gtataagccg gactgacggc gctggccctg
     1561 gcgcgttctg cgtgcctgcg caacctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     1621 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     1681 cgttgctgat tttccgcgcg gagctgagcc agcacctgct gcatatcctg taccccggcg
     1741 ctgatgatat tcagtgtgtc gacggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     1801 gcgttctccc ggcccagcga ccacgacaga cgcatggatg taatcagaag gaaagcgacg
     1861 ccaagcgtgg gccagagctt catgccggat aggctccgtg tggtaactga aaatgcggtc
     1921 cgtctttcag ggtcttccag tcgccgcccc attccaccgg aatattcagt tcctggctgg
     1981 cctgtctgaa tgctgctgcg attttttcgt acagcggcca ttcccatgac acctggttgc
     2041 cgacataagc cacaacatcc acggcatgcc ctgtgagatg gcgactgttc atggtctggc
     2101 tcttgcctgt ggtcacgagc tgcttctgac gataacgact gcgcaatcct tcggtgatac
     2161 caaaatccac ttccgagatt tccagcgccc gtcgggtcac tttcaccaga tcaggattta
     2221 caccctgcaa attcttttcg ctccggctgc tgaatttaaa tgtgttgctc attcgttcct
     2281 ctccttcacc ctgcgatcaa atattgcaat aaccttgtcg cgtgctttct ctgcgcccat
     2341 aaaaccgatt gaggcaccaa aaaacgtcac tgcatcctca gggatcccga agaagcgcag
     2401 cgacccggcc acggccatgg caagaacgcc gcacgccagc gatcccgtta cggtctgaac
     2461 cagtgttcgt ccgtcataaa gactcatcag cgcggaaatg ctgaccgccg cgcctactgc
     2521 atacaccgtt ggcaggtggt caaagagcca cgcaataacc tgctctgtga tccctgtttg
     2581 aatggtgctc actgctactc cccccacaac tgaatcattt ctcgtttctt cttctccggc
     2641 tccggcatct ccacttcctg cccggcgtcc agaaatacct gctgacagag tccggggttg
     2701 gcatccagca ccttttcggt gacgccctgt gtcgtgccgt agtaccggaa acagagcgaa
     2761 tccacggtgt cgccttccag tgccttcact ttcatcagca cagctccgca aacattcgcg
     2821 gtcgtcccag aatgtcagag atggcccagc tcacatcgcg ccacaaatcc gatgtctgta
     2881 tatccagtgc gtccgcccgg cggtcgccct tgtccgttgt gtccgcatcg cggtaacgct
     2941 ccagaatcag ggcgcgtgtg gcggtataaa cagcattgcg ccagtgccag agattgacgc
     3001 tttctccgtt aattacaggg gccggaacat cggccagcgt ctgatagcca gccgcctgtt
     3061 gttcctgctg ccatgcttcc agctcgcggg taacgtgtgc tatggccccg gtggcggtat
     3121 gcagcaggcg ggaggtggtc acgcggcccg gcagtcgtac cgccagacgc agctcgcgca
     3181 gcacaatatc cggccagaat gcacccgctg aaatacgggt atcgccatca tcggtgtcgg
     3241 tgatgtcgtc ctctgcgggg ccggggttag ttctggcaac catactcatg gggttcactc
     3301 ctgaaaaaat cgggcggtgg gtgcgcggtg taaacggtca cggagtcaaa ccggaacacc
     3361 gcgcacgccg cccgctgacg gggtcagtcg ttaaccgcgc tttgccttct gcgtcgcggt
     3421 ggtttttcgt gttgcaggct tccgcgttgt ctttttactt ttgctgcttt cgtcctgcgc
     3481 ctgctgtgcg ctggcgtctt ctggttcggc tgcggaatcg gcttttttca gggcgcggga
     3541 aagggttgca atctcgcgtt tcacacctgc gttcgggttc aggtgcatcg cttcgcgcag
     3601 cagcttcagt gatgaggcca tgttgtctgc atcggtcagg ccacgacggg caaaggcaca
     3661 cgccttgcat aatttggcgc gcacttcgtc cggcatgtcc tggttggcga taatttccca
     3721 aagtgtgtcc agtggttcga taaaggcgga taaatccgcg tcggcatccg tcccagcctg
     3781 cgtcagtacc gggttgcaga tttcttcggt cagtaccgtg gcagcagtac ggccaaagtt
     3841 atccggcatg atgaggttgt gacggaccac atatgcacca atacgcagcg ccagcggaag
     3901 atcgccgcag tcaatcgccc acaccatcag cgtggcaatt acttcgtcct gttgcccgcc
     3961 gtcagcctcc agcgttccct caatccagcc ggaaaagtcc ggcaacaact cttttttgat
     4021 ggcggctttc gcgcttctgg cctgtatggc ctttagcctg gcctgtgcca gacgcagacg
     4081 atacagcacc tcttcatgcg cggtacacgc ggcgtggtcc acaccttcat tcgcccggcc
     4141 tgcgcgctgt gccatcacgt tctgccagtg ttgttgtgca ggagtaatca ttttttctct
     4201 ccgttacagg cgggcatgat gcccgccgtg agttgattag ctgtcggcga acttcaggcc
     4261 agtgaccatc gcgcacttgc catagtcttc aacgacataa gcgtcattga tggactggta
     4321 ggtggcgatg cggttgtatt ccggctcgtc tttcatcagg cgacgcattg aacctttctg
     4381 ccagtaaatc gacaggttgt tgaacgaggt gataagcatc gttgcatccg ggaagaacgg
     4441 cgcaaggaac acgcccagcc cgccaatggt gcgcgatgac aggatgagct gcccggcaag
     4501 taattccgca ttgggattct ggccgctgat gctgttcagc acgggcagac gcagcgagtt
     4561 aaacaggttg cgccccataa tcaccgcgag gtcgtcagct tccttgtgcc attcatccag
     4621 cagggatgag cgcgcatcct gtaccagagc atcggcattc gcatacttac ccgcgtgcgc
     4681 cacagtgttg tccatgttgc gggaggtcag cgtcacgtca ttcattacgc gctcgctggc
     4741 atcggttctg atgtgctcca gccagcccac gttaacgtcc tgaagcagct tgttggtgtg
     4801 aaaatcagac tcgtaggcgt gggatgtgcc gttaaagcca atcatgatgc gatcaagcgc
     4861 tacctgccgg gcgatctgtg tactgatacg ggactgaaaa tcgctgtggg ccgcccaggt
     4921 atcaagctgt gggtacgaaa taaaggtatc gtagttcacc tgttcgcact ggtactgacg
     4981 agacttcata tcgatcacgt tattcgggtt acggcgtttg atgccgtcat aactgctatt
     5041 cgtgcgcgca attggcccgg tggtgtccag gaggattttt tcgcctttct ggtcggtcac
     5101 gccgatcacg ttaattcttt tcgtaaattc ggtactttcc tttgaggcgt tttcaaaacg
     5161 ctgctgcacc gcgggttcca cggtaaatcg cgataccagt gcagataccg ggatattgtt
     5221 aagcgacgcc tgctgcgcca tatagcaacc cagcttgttg cgggtaatat ctgacatcac
     5281 cagattcata aaaaatttgc tcctttgtct tatcagaagt cagccagctg gtcggaggct
     5341 gcgcccgttg cggtgaagcg gttctgcgga tcgccgtcct gcgtgcgcag tttttccttc
     5401 aatgctgtca gctctgtggt cagtgacgtg attttctggc ggtcctgctg atggcgggtt
     5461 tccagcacat taaaacggtc gataatgtcg gcctgtgacg ttgcaacgcc ttccaccgct
     5521 tcctgaattc gggagaaact ggcgtcatcc gctttgcggc cacgaccaat aatccccatt
     5581 acgcggttaa accactgggt gccttcttcc tggcgttgtt ctgccatttc gatgatttca
     5641 gactcgatgg cttcggagat aagcggtgct tcaccctgga tactgttgaa cgtcatcacc
     5701 gcctgacgtt gctgtgccgt gaatttcagg cgctcagtgc ccaggcttgc cggggtgtcg
     5761 gtcatcgcca gcccgaccag ataggcgcgc ccgttaacgg agaactgcgg gtgcagttcg
     5821 atactggaat agattttctt gccgtcagcg acaagctgct tcatgcgctc ggtcggttcg
     5881 atttctgcat acagcgcagt acgtccggcc agcggacctt ccgtaatgtc ttccgtactc
     5941 agtgcggtga catcgcccat tgcggaaaat tcgcttgacg gacatggcga gagatagtgc
     6001 tcaacgttca cgcgggcagc gtaaacatcc gggttgaagt tctcggcggc ttcacgcaga
     6061 tgcaccggac tgatttcacg gccatcaaca gttgatccgg agacagccac gcgaaacttt
     6121 ttgcgggatg tctttttttc attagccata gtttttgccc ctctgactgg ttcttcagtc
     6181 atgatggcaa agcgtaacag gctgatacaa agggcttttg ttgtaagaaa acagtcagaa
     6241 cagagggtta aggagaacag tttcgcgcgc gggtaatctt cctgtaatta ctcaggggga
     6301 gcaatgattc aggacgcttt tgtgcgccag cgtgcgcggc aactttactg gcagggttat
     6361 ccgcccgcag aaatatcacg tctgatggga ataaacccga acacgattta tgcgtggaaa
     6421 aaacgtgacc agtgggatga aacgccaccc gtgcagcgtg tcacgcagtc catcgatgcg
     6481 cgcctcatcc agcttactga aaaacagaat aaaacaggcg gtgactttaa ggaaatagac
     6541 ctgctgaccc ggcagcttaa aaagctgcat gatggccagc cggatgtgat ggccgcagga
     6601 aagaaaggcc gggcgaaaaa actcaaaaat catttcacgc cggaacagat tgccgcactg
     6661 cgggaaaaaa tcatcagcag gctggagtgg catcagcggg gctggtttga ctccctgacc
     6721 ctttgcaggg aagccgggat acgtaacagg atgatcctga aatcccgaca gattggggcg
     6781 acctggtatt ttgcacagga agcactgctg atggcgctgc gtgacgatgt ggcgcaacct
     6841 taccagcgta accagatttt tttgtctgcg tcgcgtcgtc aggcgttcca gtttaaaagc
     6901 attattcaga aggccgcggc tgaagttgat gtggagctga aagggggcga taaaatcatc
     6961 ctctccaacg gcgcagagct gcattttctc ggcacttctg ctgcgtcggc acagtcctat
     7021 acgggcaatt tttattttga tgaatttttc tgggtcagtc gctttgctga actgcgcaag
     7081 gtggctggcg ctatggcaac cctcagcgga ctgcggcgca cctacttctc cacgccatcc
     7141 accgaaacgc acgaggcata cgcctactgg aacggcgacc gctggaacga gaaaaaggcc
     7201 tcgcataaac gccagcgttt ttctgtggac tggaaaacgc tgcataatgg gcttatctgc
     7261 cctgaccgga cgtggcggca aattgtcacg ctggaagatg tggttaatca cggctggaaa
     7321 cacaccgata tcgacgaaat tcgtgatgaa aacaccgaag acgagttcct caatctctat
     7381 atgtgtgagt ttgtccgcga aggggaatcg gcatttaacc tgaatatcct gattggctgc
     7441 ggtgttgacg gatacgacga ctggaaagac tggaaacctt ttgctccccg cccgatgggg
     7501 aatcgtccgg tatggattgg gtatgacgca aacggcagta gtggcaacgg cgacagcggc
     7561 gctgtgtccg tggtggttcc tccggctgtt cctggcggcc gttttcgaac ggtggagacg
     7621 cgacgcgttc aggggctgga gtttgaagaa caggccagag tcattgaaga gttcacgtgt
     7681 cgctacaacg tggaacacat cggcattgat gtgacgggcg ggaacgggga ggctgtttat
     7741 cagatagtga agcggttttt ccctgctgct attccgtaca ccttcacgct gtcatcaaaa
     7801 cggtcgctgg tactgaaaat gctgcaaata atgcgtgccg ggcggtggga atacgatcgc
     7861 gccgaacgcg agctggtcgc ggcctttaac gccgtgcgta aggtgaaaac accgggcggc
     7921 tttatcactt acgaaacgga ccgcgcgagg gggatcagcc acggcgacct tgcgtgggca
     7981 accatgcttg ctgtcattaa cgaaccaatt ggcggcgaag gagaaaacga gcgtttcatg
     8041 gttatggagt tctgatgagc agaaaaaata aaaaagtgcg catgagttca cgcattgatc
     8101 tcgctgatgc gctcaggaaa gaatcatcgc tcagtgcatt cacatttgat ggtccttatc
     8161 gcctgaccgg gcatgacctg ctggacaata tgtactgtgc tgataacggg cggtggtatg
     8221 aaaccccggt ggactggtac ggtctggcaa gagcagcccg gcaaacgtcc tggcatcagt
     8281 ctgcgcttta ctttaagcgc aatgtattac tcggttgcta catcccgcac ccgctgcttt
     8341 cccggcagga tttctcggcg ctggcgctgg actggtttgt gttcggtaac gcattccttg
     8401 agcttcgaag caatatgctc ggcgaaccgc ttaaattacg gcacgccctg gcgaaataca
     8461 tgcgacgcgg aagcgatctt gaatcatggt ggtatgtgca ggatggcaag gacgcgtttc
     8521 agtttcgtcc tggcaaagtg tgccacctga tgaatccgga tattaaccag gaaatctacg
     8581 gcatgccgga atatcttggc gcattactct cggccagcct ttctcattcg gcggacatgt
     8641 tcagaaaact gtactacgac aacggatccc acgccgggtg catcatctac atcggtgcag
     8701 cgcaggtaaa ccgcgaaagc atggactccc tgaaagaaac gctacagggg gcacgtggtg
     8761 gtggtgcgtt taaaaacgtg ctcattcatg cgcccaacgg gggcaaagag ggggtgcaaa
     8821 ttttgccgtt ccagcagatc accgcaaaag atgaattcat gaatgttaag gcggcatccc
     8881 gtgatgatgt gctggctgcg caccgcgttc cgccgcaact gatgggggcg atgccgggcg
     8941 aaaaaagtgc gtttggcgat gtggagaagg cagcgcgggt ttacgcaatt aacgagctga
     9001 tgcccgttat ggaggctatg aagcacatca atgactggct tggcgaagag gtgatccgtt
     9061 ttaactctta tgctcttctt gatgaaaaaa cagccccgtg atggggctgt cctttttacc
     9121 agagttgaac cattttctgg gtgccgtcag gcttgagatt atcaatttca gagagaacgt
     9181 aatattgaat ggcttcacaa acggtggtgt agggtgaatt accttcttta agtggcacga
     9241 tattattatt aacgcgaacc tgtatttcat cgttatacat tgcgatcgaa acaggagtgt
     9301 gcacgaacgc gacttcgcca ggtgtgtcgt caaccaccgt ttcaatacta aaagtcagtt
     9361 ttcgctcatc attgctgccc cgtgctttgg gggtagcggc agggatctgg gataaaggca
     9421 ttctgcgaaa tccttcggct gcttccagtc cgcatgaaac gtaatgctgg cgattaccgt
     9481 cgctatctgt ccaggtctgt gatggcagct ccagcgagat ttcataagca tcaacaatgc
     9541 cctgggcaag gcgtacaagc ggggtcagat cttcattgcg gcgaaagctc tcctttacct
     9601 gctcccgttt ttctcttaac tgcttgtaat taatgaccat aagacagcct ccattgattt
     9661 ctttgctcgt attttgcact tatgaagtat ggtcggcaag gtgccgtatc gctgacgcgc
     9721 ttcgcttgtc tgctgcttcg ccggggcata aaaaatttat gccccggctc tccagctcct
     9781 gtatcaatca gataatttca cgacgccttc cagtttatcg ccaccatcga cggtcagact
     9841 cttacgcaat tccaccgcgt tgactgcatg ttctcgctgc ctcagtgcga ttttgacggc
     9901 cttacctttc accccatcaa atcaaaagcc ctcacgcctt tttcatgctc agcgtgagaa
     9961 atatggccat tctgttgtgt ctctgcgaca tcgttcaggg aatgctattt accccctgaa
    10021 acgcgggctg ttcccccgtc acctgcgcgc agaaaaagcg cgtttttttg tgcacgcacg
    10081 gatccttgac ggatccagcc gccatgcggg ccggaagggt aaaaagtcgt tcaaaaaaat
    10141 tgtgcaaatt tgtgcacttt tgtgcaggta agcatgccta tatatttgac cgtggcttgc
    10201 ctttgatgta gtttatacgt cacaaaaacc acaaaacgtg gttatgattt ctatgagggg
    10261 gttgatatga aaaaaatgta tgatgaattt gatggtttct aagctacgtt gacctctaat
    10321 gaggcgggga tttgctcccc gcctttttta tggatgaaat aggatgaacc caataacact
    10381 acaaatcatc agcaatgcta ttgttctgct tggcgttttg gttgccattg gaaccattat
    10441 ctacaatgtg cgtaccgcaa aaaaaactca aacggccaac tttctttttg agagtcggca
    10501 agatatgcaa tatatagagt cattgcatac cctgaaacaa gtgcatcgtt caggcaaatc
    10561 atttcgttct tatgtttttc cttgtgatgg ttgcataata acagatgaag aaatggccga
    10621 gcgtcgaaaa tttcaatata t
//
LOCUS       DAPQZD010000166        10349 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPQZD010000166 DAPQZD010000000
VERSION     DAPQZD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.168"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..476
                     /locus_tag="RO454_004090"
     CDS             <1..476
                     /locus_tag="RO454_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDY0935840.1"
                     /translation="KLIIISDNFRKLAKNADYNGPELFTHAHQFVGQNYLGFGDYTIT
                     GQVLDLGGGQPSAVAAHLVFKNLQANTVWIRHFVSSNTQRGSSNVTAKFLDVSDQITN
                     LVPQHPTQFGSNIGLNYYYYNSQPTVRHFPGLPKNKQYQITHHICFMLDLIAGRI"
     gene            complement(456..1631)
                     /locus_tag="RO454_004091"
     CDS             complement(456..1631)
                     /locus_tag="RO454_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDY0935841.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTAGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARTKRDEARSMLANGINPSEARKADKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPEFVNA
                     LDNYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAINILKKLQEITGNYSLVFPGRNDVSKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYIKDRQVMMEWYASLLNTTCY"
     gene            complement(1792..1866)
                     /locus_tag="RO454_004092"
     tRNA            complement(1792..1866)
                     /locus_tag="RO454_004092"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1831..1833),aa:Arg,seq:cct)
     gene            complement(1942..2874)
                     /locus_tag="RO454_004093"
     CDS             complement(1942..2874)
                     /locus_tag="RO454_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDY0935842.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3168..3923
                     /gene="mlaA"
                     /locus_tag="RO454_004094"
     CDS             3168..3923
                     /gene="mlaA"
                     /locus_tag="RO454_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDY0935843.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3985..5325)
                     /gene="fadL"
                     /locus_tag="RO454_004095"
     CDS             complement(3985..5325)
                     /gene="fadL"
                     /locus_tag="RO454_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDY0935844.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            5697..5981
                     /locus_tag="RO454_004096"
     CDS             5697..5981
                     /locus_tag="RO454_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDY0935845.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6162..7472
                     /gene="fadI"
                     /locus_tag="RO454_004097"
     CDS             6162..7472
                     /gene="fadI"
                     /locus_tag="RO454_004097"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDY0935846.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7472..9616
                     /gene="fadJ"
                     /locus_tag="RO454_004098"
     CDS             7472..9616
                     /gene="fadJ"
                     /locus_tag="RO454_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDY0935847.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            9819..10304
                     /gene="sixA"
                     /locus_tag="RO454_004099"
     CDS             9819..10304
                     /gene="sixA"
                     /locus_tag="RO454_004099"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDY0935848.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2567 a         2455 c         2615 g         2712 t
ORIGIN      
        1 cgaagttaat tattattagc gataacttta gaaagttagc aaaaaatgct gattataatg
       61 ggccggaact tttcacgcac gcacatcaat tcgtgggaca aaattactta ggatttggcg
      121 attatacaat tactggccag gtactagatc ttggaggtgg tcaaccttct gctgttgccg
      181 cacatttggt gttcaagaat ttacaagcga atacagtttg gattcgtcat tttgtatcaa
      241 gcaacacgca acgaggaagt tcaaatgtta ctgcaaaatt tttagatgtt tcagatcaaa
      301 ttacaaatct tgttccccaa catccaaccc agtttggcag caacattggt cttaactatt
      361 attattacaa tagtcaacca actgtaaggc atttccctgg gttgccaaaa aacaaacagt
      421 atcaaattac tcaccatatt tgtttcatgc tggatttaat agcaggtcgt atttaataag
      481 ctagcgtacc attccatcat tacttgtcga tctttaatat attgcgcatg attgtaagta
      541 cctcgaatag agttcttatc aacatgcgca agctgcattt caatccaggc actttcaaac
      601 ccatgctcat gcaagatggt gctcatggta tgcctgaaac cgtgacccgt taaccgaccg
      661 tgataaccca gtagttttat cactttgttg atgctggctt cgctcattgg tttactgacg
      721 tcattccggc cagggaaaac gaggctgtaa ttccctgtta tttcttgcag ctttttcagg
      781 atattgatcg cctgagttga taacggaacc agatgggggc ggcgtttctt catccgttct
      841 ttggggatct cccataaggc attatctaaa tcaaactcag cccattccgc tgcacgtagt
      901 tcaattgttc gcacgcctgt tagcatcaac agctgcgtgg cgtatttggt aactaaactg
      961 ccctgatagt tatccagagc attaacgaac tcgggcaact cgctttcagt caggaacggg
     1021 aagtgttggg ttttaggttt gttgagtgcg atagctaaat cgggtgcaaa attatacttt
     1081 gctctaccag tggcaacagc ataacgaagc acttcaccac agcggcggcg gatcttactg
     1141 gtttgctcta gcgcacctct tttctctatt ttttgcagga cggttagcag ttctaatggt
     1201 tcaatctgct caattgggcg ctggccgata aaaggaaaaa tatccttttc catacaattg
     1261 agaacctctt ttgcataacc ctctgaccag gtggcttttt tcgatgagtg ccattctctg
     1321 gccacagact caaagctgtt ctcatgcgcg aactgcaaag caatcttatc tgcttttctg
     1381 gcttcacttg ggtttatccc gttggctaac atcgaacgcg cttcatcacg tttggtacgt
     1441 gcctgtgcca acgacacatc gccatatacg ccaaaggaaa tcatcttagg cttacctgca
     1501 aagcgatagc gaaaacgcca gcctttactg cccgctgtgt cgatcaggag tgatagcccc
     1561 ataccatcgt taagcgtata gggtttgtcc ttcggttttg ctctttttat ttggatgtca
     1621 gatagcagca tgtgtataga aaaaagatcg aactcagtta tacacaatgt tatacgcaaa
     1681 gatgtataga ttccattaga aataatggta ctttgctgga cgagtataag gagatgatat
     1741 cagttaaaac agtgagttat ggaattaatt gaacttagtg agaagtgttc atggtgtccc
     1801 ctgcaggaat cgaacctgca attagccctt aggaggggct cgttatatcc atttaactaa
     1861 gaggacaatg cggcatgagt atacccgcta atggactacg gggtaagtac acagccgctc
     1921 gattgcttaa accctcgcca tttatgccgg gtttttatca tttttcttaa tgttttccgc
     1981 acgttctgct ttttgacgtg cttctgcttt gcgcttgttg ctcatgtcgt tacgaatctg
     2041 tgcatgactc attaacgcga agataaaggt gccgccgcag atgttccccg ctaaagtagg
     2101 tagtgcgaag ggccagatga aatcgctcca gtgcagcgtg ccgttaaaca ccagatagag
     2161 gatttcaaca gaaccgacaa cgatgtgggt ggtgtcaccc agggcaataa gccaggtcat
     2221 caatataatc accacaatct ttgccgcacc cgctgcagga aacatccata ccatagtggc
     2281 gatcagccag ccggaaataa tcgcgttggc aaacatctcg ctgggggtgt tcttcatcac
     2341 atccatgccg attttgacaa atgcatcgcg agtttcttca ttgaagatag gcatatattc
     2401 aaatgcccac gcagcaatac ctgtcccgag aatattaccc agcagcacga tgccccataa
     2461 ccgcataagt aagccgacgt tgctcattgt cggtttttgc atgacgggta gtaccgcagt
     2521 tacggtgttt tcggtaaata attgctggcg ggccattatg acgataataa aaccaaaggt
     2581 ataaccgaga ttctccagca agaagctgcc cggcacgcct tccagttcga catgaaatat
     2641 cccttttgcc agtaacgaag cgcccatcga caggcccgcc gcaatggctg accacagtag
     2701 cgccattgcg tcgcgttcca gctctttttc accatcctgg cggatatgct catgaattgc
     2761 catcgcccgg gaggggagtc ggtcttcatc tatttctatt tttttaccgc gctctttttc
     2821 ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt ttgtcgttgt ccatggtctc
     2881 ttctcttgaa ttagcacgta tagctaaagc gtagcggctt ttttgctcgc aactggcggg
     2941 agttactctg aaaatgtaga aaaggccgcg tttgcctttt tctgtttcta ttgaatcaag
     3001 tagcctacag ggcggcgatt accaggctat gatcaaatca gcaaatcagg gcgtctggac
     3061 atcagttgac gtgctgttac aatcgcccac acctaaacag gcggatacgg tatcgttccg
     3121 tcatggatgg caaactgcat aagccataaa aaaacaggga gacatttatg aagcttcgcc
     3181 tgtcggcgct tgctctggga actacgcttc tggtggggtg tgcgagttcc ggtacagatc
     3241 agcaagggcg ttctgacccg ttagaagggt ttaaccgcac tatgtacaac ttcaacttca
     3301 atgtattaga cccgtatatt gttcgaccgg tcgctgtcgc ctggcgtgat tatgttccgc
     3361 aacctgcgcg taacggtttg agcaacttta ccggcaacct tgaagaacct gcggtgatgg
     3421 ttaactactt cttgcagggc gacccttatc aggggatggt ccactttacc cgctttttcc
     3481 tgaataccat tttgggaatg ggcggcttta ttgatgttgc agggatggcg aacccgaaac
     3541 tgcaacggac tgaacctcac cgcttcggta gtacgcttgg tcattatggt gtgggttatg
     3601 ggccgtacgt tcagttaccg ttctacggta gcttcacgct gcgtgatgac ggtggtgata
     3661 tggcggatag tctttacccg gttctttcct ggctgacctg gccgatgtct gtgggtaaat
     3721 ggacgcttga agggatagaa actcgtgcgc agttgttgga ttccgacggt ctgctgcgtc
     3781 agtcctccga tccttatatt atggtgcgtg aagcgtactt ccagcgtcat gatttcatcg
     3841 ctaatggcgg tgaactcaaa ccgcaggaaa atccgaacgc gcaagcgatt caggatgatt
     3901 taaaagatat cgattctgaa taagaaacaa acaaaaaagg tgagtcgcaa aactcacctt
     3961 ttttcttatt aagctaacgc gttatcagaa cgcgtagtta aagttagtac cgaacagcca
     4021 ggctttacct tcagactcga aggaataagc tgccgctcct tcaccttcgg tgaatttcac
     4081 tttctgacca tgcatataag atgcgccaac atcaatggat gcatcttcat taaatgcgta
     4141 ggttaaccca gcacttaacc acaaacgatc ctggtcagga atggaaatag aacgtttatc
     4201 tgccggaacc gggctgtcgt cataagcgat accggtacgg aaggtccagt ttttatcata
     4261 atagtaggtt gtacccaacg cgatacgata cgcgtcccgg aatccttcat ctttatagaa
     4321 cagagtctga ccactatcgc ctttcgcttt caattcctgg aactggctcc aactggtata
     4381 ggccatgctg tagtgaattg cccactgcgg cgcaacacgg ttataaccag aaatctccca
     4441 catctcaggc aggttcagcg ttaaggatcc attttgggtt gcccccatcg tgccatacgg
     4501 taagcccatg ttacccaaaa tattgttcag atttgggtta atggagcttt tgtaatcccc
     4561 atcgaagtcg attttaactt ctgaacgata tgtcagcccc caacggttat ctttatccag
     4621 ttcataaaga atacccgcgt tccagccaaa gccccattcg tcacctttca aatgagcaat
     4681 ttgagagtcc ggagtattaa taccattggt taatgaagcg ggtacaccct gggcaatcaa
     4741 ctgttgagac aacaatggcc ccagcgaacc ggcgtaacgc tcaatttttg ccttggcata
     4801 aactgcatca aaaccaagac caaaactcca gctattgtta aggcgataag ccccgctaag
     4861 gttaaggttg attgtttgca gatcggtttt accgccatag ataccagcag cgtagttgtc
     4921 ggtaaattcc gtagccaggc catagttgga agttattgac gctccccaac caaactgatc
     4981 gttaataggc gcgacaaagt gcaggttagg tacccacgct gtcggcgcga tatttttagc
     5041 atttgcactc tgaccggtta aattagaatg tccggtaata tctacatccg gatcaatata
     5101 aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta atcaatgcgg ggttacggct
     5161 aacgttacct gcatcatcgg caattgcacc ttcccctgaa taagcccggc ccaggccaga
     5221 ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc tgggtggaga taagtgccac
     5281 tgcgactgcg agagcagact ttgtaaacag ggttttctgg ctcatgacca taacctcaat
     5341 gatttatttt tatacaaact atgttacgtg ctgtaacagg agcgcgaagt gtagggtctg
     5401 aggtacatct tagaaatcag accagtggcg agagtatagg tcggaccagc tgggatgttg
     5461 caagtatgtt tctatctttt ttcagatatg atctaagaaa cgcgatccag ctggcaaaat
     5521 tgggaggtgg cgcactcatg gattcgggtg aattttgttt tagatcattt ttaagtgtga
     5581 tttcggtcac ttatccgatt ttgcagaatt aacaactgga gcagcacttt ccggaagcgt
     5641 aaaatatcca caacgtttat ctggcacctt gggtgcacat acagaggaaa tcaacgatga
     5701 gtaaatgtag tgctgatgaa accccggttt gctgctgtat ggatgttggc accattatgg
     5761 acaactccga ctgcaccgcg tcttacagcc gtgtgtttgc aaaccgcgca gaagcagaac
     5821 aaacgctggc ggcgctgact gaaaaagccc gtagcgtgga atccgaaccg tgcaaaatca
     5881 ccccgacttt cactgaagag tctgacggtg tgcgcctgga tatcgatttc acttttgcct
     5941 gcgaagctga aatgctgatc ttccagctgg gtctgcgtta attcctctcg ggtacatacg
     6001 cccctgtcag ctgctgacag gggcgtttta ttttctcctc ctcttttctc tctgtgtcat
     6061 agctcccact ttcccttttc tccacttggc taaatgtaaa aaaatggtta agactatgat
     6121 caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag gttttaccgc
     6181 tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg ccttttgccc
     6241 gacaggcgac ggcttttcat ggcattcccg cggttgattt agggaagatg gtggtaggcg
     6301 aactgctggc acgcaccgag atccccgctg aagtgattga acaattggtc ttcggtcagg
     6361 ttgtgcaaat gccggaagcg cccaacattg cccgtgaaat tgttcttggc actggaatga
     6421 atgtgcatac tgatgcttac agcgtcagcc gcgcttgcgc caccagtttt caggcggttg
     6481 cgaacgttgc cgaaagcctg atggcgggaa ctattcgagc ggggattgcc ggtggggcag
     6541 attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgcgcgta ctggttgatg
     6601 tcaacaaagc ccgcacaatg agccagcgcc tgaaactctt ctctcgcctg cgtttgcgcg
     6661 acttaatgcc cgtaccgcct gcggtagcag aatattctac cggcttgcgg atgggggaca
     6721 ccgcagagca aatggcgaaa acctacggca tcacccgaga acagcaagat gcactggcgc
     6781 accgttcgca tcagcgtgcc gctcaggcgt ggtcagaagg taaactcaaa gaagaggtga
     6841 tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat attcgcggta
     6901 attcctctct tgctgattac gcaaagttgc gtccggcgtt tgatcgtaaa cacgggacgg
     6961 taacggcggc gaacagtacg ccgctgaccg atggcgcggc agcggtgatc ctgatgactg
     7021 aatcccgggc gaaagaatta gggctggtac cgctgggcta tctgcgcagc tacgcattta
     7081 ctgcgataga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca ccgctggcgc
     7141 tggaacgtgc cggattgacg atgggcgatc tgacattgat cgatatgcac gaagcctttg
     7201 ccgcccagac gctggccaat attcagttgc tgggtagcga acgttttgct cgcgacgtac
     7261 tggggcgtgc acatgccact ggcgaagtgg acgagagcaa atttaacgtg cttggcggtt
     7321 cgattgctta cgggcatccc ttcgcagcga ccggcgcgcg gatgattacc cagacattgc
     7381 atgaacttcg ccgtcgtggc ggtggatttg gtttagtgac cgcctgtgct gcgggtgggc
     7441 ttggcgcagc aatggttctg gaggcggaat aatggaaatg gcatcagcgt ttactcttaa
     7501 tgttcgtctg gacaacattg ctatcatcac catcgacgta ccgggtgaga aaatgaatac
     7561 cctgaaggcg gaatttgcct cgcaggtacg cgccattatt aagcaactcc gtgaaaacaa
     7621 agagttgcgt ggcgtggtgt ttgtctccgc gaaaccagac aacttcattg ctggcgcaga
     7681 catcaacatg atcggcaact gcaaaacggc gcaagaagcg gaagtactgg cgcggcaggg
     7741 gcaacagtta atggcggaga ttcatgcttt gcccattccg gttatcgcgg ctattcatgg
     7801 cgcttgcctg ggcggcgggc tggagttggc gctggcgtgc cacggtcgtg tttgtactga
     7861 cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac ccggttcagg
     7921 aggtacccag cgtttaccgc gcctgatagg tgtcagcaca gcattagaga tgatcctcac
     7981 cggaaaacaa cttcgggcga aacaggcggt aaagctgggg ttggtggatg acgtcgttcc
     8041 gcactccata ttgctggaag ccgctgttga gctggcaaag caggatcgcc catcttcacg
     8101 ccctctacct gttcgcgagc gtattctggc ggggccgtta ggtcgtgcgc tgctgttcaa
     8161 aatggtcggc aagaaaacag aacacaaaac gcaaggtaac tatccggcga cagaacgcat
     8221 cctggaggtt gtcgaaacgg gattagcgca ggggaccagc agtggctatg acgccgaagc
     8281 tcgggcgttt ggtgaactgg cgatgacgcc gcaatcgcag gcgctgcgca atatcttttt
     8341 tgccagtacg gacgtgaaaa aagatcccgg cagtgatgcg ccgcctgcgc cattaaacag
     8401 cgtggggatt ctaggtggtg gcttgatggg cggcggtatt gcttatgtca ctgcttgtaa
     8461 agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc atgcgctgaa
     8521 gttcagttgg gatcagcttg agggcaaagt tcgccgtcgt catctcaaag ccagcgaacg
     8581 tgacaaacag ctggcattaa tctccggaac gacggactat tgcggctttg cccatcgcga
     8641 tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg tggcggaagt
     8701 ggagcaaaat tgcgctactc ataccatctt tgcttcgaac acgtcatctt taccgattgg
     8761 cgatatcgcc gctcacgcca cgcgacctga gcaagttatt ggcctgcatt tcttcagtcc
     8821 ggtggaaaaa atgccgctgg tggagattat tcctcatgcg agtacatcgg cgcaaaccat
     8881 cgccaccacg gtaaaactgg cgaaaaaaca gggcaaaatg ccaattgtcg tgcgtgacaa
     8941 agctgggttt tacgtcaatc gcatcttagc gccttacatc aatgaagcta tccgcatgtt
     9001 gaccgaaggc gagcgtattg agcatatcga tgccgcgcta gtgaaatttg gttttccggt
     9061 aggcccaatc caacttttgg atgaggtagg aatcgacaca gggactaaaa ttatgcctgt
     9121 actggaagcc gcttatggag aacgttttag cgcgtctgca aatgttgttt cttcaatttt
     9181 gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg gtcagaaagg
     9241 gcgtaaaagc aaaaaacagg tcgatcccgc catttacccg ctcattggcg tacaagggca
     9301 ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc tgaatgaagc
     9361 agtacgttgt ctggacgagc aggttatccg tagcgtgcgt gacggggata ttggtgcggt
     9421 atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg attctctcgg
     9481 cgcaggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt cccgttttac
     9541 cccttgcgat cgtttagttg agatgagcga gcgtggggaa agtttttgga aaacaactgc
     9601 aactgacctg caataagaag gtcaaagtta tatgaatccg cgctgaatgg cggagtgttg
     9661 ctcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa cagcgtttca
     9721 ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt ttcagatgaa
     9781 agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt atcatgcgtc
     9841 acggcgacgc agccctcgat gccgccagtg attcggttcg tcctctgacc actcatggtt
     9901 gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa atcgaacgcg
     9961 ttctggtgag cccgttcctg cgagccgagc aaacacttga agaagtaggg gattgtctga
    10021 acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat gttggtctgg
    10081 tcggtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg gtgatctccc
    10141 acttaccgtt agttgggtat ctggttgccg agttatgtcc gggcgaaacg ccgcctatgt
    10201 ttaccacgtc agcgattgcc agcgtaacgc ttgatgaaag cggaaaaggc caattcaact
    10261 ggcagatgag cccttgtaat ttgaagatgg caaaagcaat ttaagcaatc aatgcccggt
    10321 tacgtgcgcg tacaccgggc agtttctcc
//
LOCUS       DAPQZD010000167        10293 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPQZD010000167 DAPQZD010000000
VERSION     DAPQZD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.169"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(99..1484)
                     /gene="atoC"
                     /locus_tag="RO454_004100"
     CDS             complement(99..1484)
                     /gene="atoC"
                     /locus_tag="RO454_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDY0935849.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(1481..3307)
                     /gene="atoS"
                     /locus_tag="RO454_004101"
     CDS             complement(1481..3307)
                     /gene="atoS"
                     /locus_tag="RO454_004101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDY0935850.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            3474..6323
                     /gene="rcsC"
                     /locus_tag="RO454_004102"
     CDS             3474..6323
                     /gene="rcsC"
                     /locus_tag="RO454_004102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDY0935851.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6523..7173)
                     /gene="rcsB"
                     /locus_tag="RO454_004103"
     CDS             complement(6523..7173)
                     /gene="rcsB"
                     /locus_tag="RO454_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDY0935852.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(7190..9862)
                     /gene="rcsD"
                     /locus_tag="RO454_004104"
     CDS             complement(7190..9862)
                     /gene="rcsD"
                     /locus_tag="RO454_004104"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDY0935853.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2521 a         2411 c         2621 g         2740 t
ORIGIN      
        1 aatagaaaag gataaattag atgggggttc ataaggagaa tgagcggcta tgcagaaaat
       61 tgcgcactgt gcaaatttct gcatagcaag ttttggcatt atacatccgc cggatcgata
      121 ccgtattcct ggagtttata catcaatgca cggcgactga tgcccagcat taaagcagtg
      181 cgggttcggt ttccttcttg ttgttccagc acttccataa tgatgcgttt ttcgacgcgt
      241 ttaatttcct cttttaaatt acgctcaccg acaggggctg tttttgcttc accagcatta
      301 cagactggct gacgaatctg cggcggaaga tcctcagaaa aaatgatcgg gcctgaattc
      361 atcacgacgg cgcgttcaat aacgttggaa agctctcgaa tatttcccgg ccatgaccag
      421 gcggtaagca gtgacattgc catcggatcg atgtcgataa tatcgcgctg attctcacta
      481 ctgaattttt gcaaaaagtg attagctaac agggaaatat cttcccggcg atcgcgcaga
      541 ggcggcagta ttaaatgaat aacgttaagg cgataaaaga gatcttcacg gaaggtgcct
      601 tctttcacca ttgcctgcaa gtcgcggttg gtggcagcaa tgacgcggat atcaactttt
      661 atggtctgat ggccgccaat ccgttcaaat tcccgttcct gcagaatgcg tagtaatttg
      721 gcttgcagta ccagcggcat ttcgccaatt tcgtcgagga gcagagtacc ttcgttggca
      781 cgttcaaata atccctgacg caaggtttgt gcaccagtaa atgcaccttt ttcatgacca
      841 aacagttcac tttcgagcaa cgattccggc agtgccgcgc agttgacttt aatgaacggc
      901 ccctttgccc gccgcgaatt gtagtgaatc gctctggcaa tcaactcttt cccggtgccg
      961 ctttcaccgc taatcaagac gctggcctga gaaagggcaa ttttggcggt gtctttgcag
     1021 atgtccatca tcgccgggct gttggtgaga atgtgccccc attgccagct ggtgctcagt
     1081 gcctggtgca gatgacggat ctcttttttc attgactgga gttgtaaagc gcgctgaacg
     1141 attaaattca actcatcgag atcaaacggt ttgataacat agtcgaaggc tccgcagcgt
     1201 agtgcttcga cggcggtttc cacttcagca taggccgtca tcagaataac gggtgtccgg
     1261 gtctcatggc tgcgcatctc ctttagtgcc ttgatgccgt ccatctctgg catgcggata
     1321 tccatcaaca ccacgtcagg gtgaatatcg gcaaacaggt gtaatgctgt gcgcccgttg
     1381 ttcgcacaat gtgtttcgaa tccttgtagt gcaaaagcgg tgctcagcat acggcgaaca
     1441 ttatcttcat catccacaat aaggatgcga ttaatagcag tcatacagtc tgatttccct
     1501 gcgggttgat cggtaaaata agcgtgaagg ttgcgccgta gcccggcaaa ctggcgacgc
     1561 gaatatcacc ctgatgggca ttaatgatgc gttgacttaa cgccagacca agcccggttc
     1621 ctgaggcttt ggtagtgaaa aagggatcga agatcttttt ttgcagcgag agatcaatgc
     1681 cactgccgtt gtcctctatc gaaatggcct gttgcgagtc gctatactgc caggttcgaa
     1741 tgcgaatttt ccctcgtgca ctgatagcct ggacggcatt gatcaggata ttcagtagta
     1801 cctgtttgag cagttcacga tcggcgttaa tcgggcttaa ttcattatcc agttcgctta
     1861 tgaagtcgac ccgcgcttgt acgccggcgg tttgtaccag taccagagtt tcttcaacca
     1921 atgcattgag gctgacttgt tgccattgac tgtggcgtgg gcgtgaaaat tcgagcaatt
     1981 gctgaataac tttgttaatt gaatcgattt ctttgagtac tacggacaga tattcctgat
     2041 gtattgggtc acgggtttgt tggcgcaaga tctgtacata accacgaata gccgttagcg
     2101 gattacgtac ttcatgcgcc acgccagcca tcagctcacc cagtgtggcg aggcgttctg
     2161 cttgcgccat gcggcgctgg gtttctttgc gggcagttaa atcagagaaa atcaccaaag
     2221 cacctatcat ttcaccgtgc gtgttatgaa tacgactggt cgtgacactg agttcaatgg
     2281 tgcggtcacg gcctggaaaa ctgatctcca gtgcaacatg ttcggtgcca tgttccagcg
     2341 tatccagtac gggactgtag aactgagtat tgtcgaacaa catggagtaa ggctgcccta
     2401 ccagttcatg acgttgatag ccagtgatga cttctgctgc tgggttcatg gtggttacat
     2461 caccctggcg gtcaatggca atgacgccat cggcagcgtt ttcaataatc agatcgttaa
     2521 gtgttcgcgt ttcacgcagt gcctgggcga ggttgttaac actctgactg atttgcccca
     2581 tttcaccggg caattgtggt aatcgagtgg gaatattttg agccagagtc gagaggccat
     2641 cggtgatgat atctatattg gcgctcaggc gacgggagaa aaggacaatc aacagcaggc
     2701 ttatcagcaa accggcggtg agcacaatga taatcctcac atccattttc caggcctggc
     2761 ggcgaatatc ttcggttaat tcattggccc agatatagcc gaggatttca ccattacgct
     2821 caatgggaat cattgaattc aaaatatcgc cgcgcacctg cctgcctgaa taaaccaaag
     2881 gggtatttgt acgcatgact tcgcgaccag ggtgatctgc ggcaatggta acgcccacat
     2941 tattctgata tagcgctgaa ggcgcgtagg tgattatcgc atccagcgtt ttgttgtaat
     3001 aaccagcgcc gatgccaggg aaggcgtgag tgatattttc ggtaatgggg gcaagttctg
     3061 catttaatgc gcggatacgc tcctcacgcg gtaagtcgat gtagagatcg tagcgattgc
     3121 ctagcgcctg attaagcagg ttgaccacgg cagaaagttt tttctctttt tcagataaga
     3181 ctgctgaacg tccttccgtt tctacgatat aaccaatagt aagcgttggg acaatgacca
     3241 tcaggattgc catcaggatc atttgattgc gtaagcggcg tggataaatc cacttcatat
     3301 aatgcatgct gaaatccact agtcttgtcc ggtatatgac gattatcaga ggttaaggtg
     3361 atgatttctc ggcggtgtat catattccag agaagagaga acattgcggt aacacgcttt
     3421 taccgctacc ttaaccacac tccatcggtc acctgaggcg gagcttcgcc cctttgaaat
     3481 accttgcttc ttttcgtaca accctgaaag cctcgcgcta catgttcaga gcattggcgt
     3541 tagtgctctg gctgttgatt gctttttcat ccgtttttta catcgttaat gcgttacatc
     3601 agcgagaatc ggaaattcgt caggaattta atctgagttc cgatcaggct cagcgcttta
     3661 ttcaacgcac ctctgatgtg atgaaagagc tgaagtacat agctgaaaat cgcttatcgg
     3721 cagaaaacgg tgtgctttcc ccgcgtggac gagaaacgca gacggatgtg cctgcgtttg
     3781 aaccgctgtt tgctgactct gattgttccg caatgagtaa cacctggcga ggttctctgg
     3841 agtcattggc gtggtttatg cgctactggc gcgataattt ttctgcggct tacgatctaa
     3901 accgggtatt tttaatcggc agcgataacc tctgcatggc caatttcggt ctgcgtgata
     3961 tgccagtgga acgcgatacc gcgctaaaag ctttgcatga acgcattaat aaatatcgaa
     4021 atgcaccaca agatgatagt ggcagtaacc tctactggat cagcgaaggt ccgcgccctg
     4081 gcgtcgggta tttttacgcc ttgacgccag tttatctggc gaaccggttg caggcgcttt
     4141 tgggtgtcga gcaaaccatc cggatggaga actttttctt acctggtacg ttgccgatgg
     4201 gggttaccat tcttgatgaa aatggtcata ccctgatttc gcttaccgga ccagaaagca
     4261 aaattaaggg cgatcctcgc tggatgcagg aacgctcctg gtttggctat acggaagggt
     4321 tccgggaact ggtgctgaag aaaaatctgc caccctcatc gctaagcatc gtttattcag
     4381 tgccagttga taaggtgctg gaacgcattc gcatgttgat ccttaacgca attttgctga
     4441 atgtgcttgc cggagctgca ttgtttactc tcgcgcggat gtacgagcgg cgtattttca
     4501 ttccggcgga aagcgacgcc ctgcgactgg aagaacatga gcagtttaat cgcaagattg
     4561 tcgcctccgc gccagtgggt atctgcattt tgcgtaccgc tgatggcgtc aatattttaa
     4621 gtaacgaact ggcgcatact tatctcaata tgcttacgca tgaggaccgc caacggctga
     4681 cacaaattat ctgtgggcag caggtcaatt ttgttgatgt cctgaccagc aacaatacca
     4741 atctgcaaat cagcttcgtc cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc
     4801 tggtggatgt ttcttcgcgc gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag
     4861 cggaacaggc gagccagtca aaatcgatgt tcctggccac cgtcagtcat gagctgcgaa
     4921 cgccgctgta tggcattatc ggtaacctgg atctgttgca aaccaaagag ttaccgaaag
     4981 gcgtcgatcg tctggtgacg gcaatgaaca actcttccag cctgttgttg aaaattatca
     5041 gcgatattct cgatttctcg aaaattgaat cagaacagtt gaagatcgaa ccgcgtgagt
     5101 tttcaccgcg tgaagtgatg aaccacatca ccgccaacta tttaccgctg gtggtacgca
     5161 agcagttagg cttgtactgc tttattgaac cggatgtgcc agtggcctta aatggcgacc
     5221 cgatgcgttt acagcaggtc atctccaacc tgttgagtaa cgccataaaa ttcaccgata
     5281 ccggctgtat agttttgcat gttcgcgctg atggcgatta tctctctatc cgtgttcgcg
     5341 ataccggcgt ggggataccg gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg
     5401 tcggaacggg cgtacagcgt aacttccagg ggactggtct gggtctggcg atttgtgaaa
     5461 aactgatcag catgatggac ggcgacatct cggtagattc agaaccggga atgggcagcc
     5521 agtttaccgt gcgtattccg ttatacggcg ctcagtaccc gcagaaaaaa ggtgtggaag
     5581 ggttgagtgg taaacgctgc tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg
     5641 aaaccagttt gcagcgcagc ggcatcgtcg ttacaacata cgaagggcag gaaccgactc
     5701 ccgaagatgt gttaattact gacgaggtag tgaacaaaaa atggcagggc agagcggtag
     5761 tgaccttctg tcgtcgccat attggtattc cgctggagaa agcgccaggg gagtgggtac
     5821 atagtgtggc tgctccgcat gagctaccgg cattgttggc gcgtatttat ttgatcgaga
     5881 tggagagcga cgatcctgct aacgctctgc cgtcgacgga caaagcggtc agcgataatg
     5941 acgatatgat gattctggtc gtggatgatc atccgattaa ccggcgtctg ctggcagatc
     6001 agttgggatc gttgggctat caatgtaaaa ccgcgaatga tggcgtcgat gcgcttaatg
     6061 tacttaacaa gaatcatatt gatatcgtgc ttagcgacgt caatatgcca aatatggatg
     6121 gttaccgctt gacgcaacgc attcgtcagt tgggactgac gttgccggta atcggagtaa
     6181 ctgctaatgc gttggctgaa gagaagcagc ggtgtctgga gtccggtatg gacagctgcc
     6241 tgtcgaagcc ggtaacgctg gatgtgataa aacagacgct gacggtatat gccgagaggg
     6301 tcaggaaatc gcgggaatcg taggacggat aaggtgttta tgccgcatct ggcattcagt
     6361 gcaaatgcca gatgcgacgc tgacgcgtct tatctggcct actttaatgt aatctgttga
     6421 aataatggga atcgtaggac ggataaggcg tttacgccgc atccggcaat ccgtgcaaat
     6481 gccagataag acactgacgc gtcttatctg gcctacaggt gattagtctt tatctgccgg
     6541 actcaacgtc acagaagaga gataattcag cagagcaata tcgttctcga cacccagctt
     6601 catcatcgca gatttcttct ggctactgat ggttttaata ctgcggttca gctttttagc
     6661 gatctcggtc accaggaagc cttccgcaaa caggcgcaga acttcactct cttttggcga
     6721 gagacgcttg tcaccgtaac caccagcact gattttttcc aacaggcgag aaacgctttc
     6781 cggggtaaat ttcttccctt tctgcagcgc ggcgagagcc ttcggcaaat cggtcggtgc
     6841 accttgtttc agcacgatcc cttcgatatc cagatccaat accgcactaa gaatcgccgg
     6901 gttgttgttc atggtcagaa caatgatcga caggcttggg aaatggcgct tgatgtactt
     6961 gattaaggta atgccatcac cgtacttatc gccaggcatg gagagatcgg taatcaacac
     7021 atgcgcatcc agtttcggca gattgttgat cagtgctgta gagtcttcaa attcgccgac
     7081 aacattcacc cactcaattt gctcaagtga tttgcgaata ccgaacaaga ctatcggatg
     7141 gtcatcggca ataattacgt tcatattgtt catgtaatag gctaccttgc tacagcaagc
     7201 tcttgacata actgtcaatg tcgctgatgt atttttctat tcctggaaca tccttctcac
     7261 gaatcagatg ttccagcgtt tcacataact gcttgccggg taccagattt agcatggcaa
     7321 atacgccttt aagacgatga gccgtttggg ctaacgcagc aaagtcgctg gttgctgctt
     7381 cagtatatag cctcttaaca tcatccggta ctgtgtctac aaagagcgca taatagccgc
     7441 tggcatggag ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg
     7501 ccagttgcac ttcaattaat tgtaagaccg cttcctgcat agcgttgctc atattgaaat
     7561 tgacgcacaa ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg cttaaaagca
     7621 agccagaggc agtaagatta gacggattat ccgttaaaaa gatatcataa tcttgactaa
     7681 ttaatctttc atcgggtgtg atacaggttg caccccaatt ttctaactgg cgagtgacaa
     7741 tattccgaat ttctgccgaa gtaacatcca ccattacgca gacatcatcc agtaatcgct
     7801 cttcttcctc ttcaacttcc gggtcagctg cgagcatttt gatatgcaca gagtagcgtg
     7861 taccaagccc atcccgcgtt ttgatgttta aatgaccgcc cagtttacgc gccagttgat
     7921 cgctcagcca gaatgccagc gggtccgcct tgccatagcg atcgttttgg gtctggttga
     7981 taaacgggaa gtgcaaatta tccatttcat gaatgcttac gccttctcca gtgtccagaa
     8041 tgcggaacgt caggcggtct tcggaggact catcctgatc aacctcaagg gtgatttttc
     8101 ctaattgcgt tgaggtcacg gcatattgca tcagtagcag caaaatacgt cgtaaggcat
     8161 cgcgatcgcc gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc agttgcagac
     8221 ctttacgctt gatggcaggc aacactgaag gcactacttc atcaattaaa tcctgcacgg
     8281 agaacagctc cgtctcactt ttccagctat cgtccgcaag catgttcgct aactgtattt
     8341 catcgaccag tcgcaccagc acatcagcct gattcgccag ttgtttgcct tccggggcgt
     8401 tgagtttagc cgcgctctcc gccagggact gtgcgggttc tttcagcgca tcgccaatgt
     8461 ttttcataaa ggtcatccgc ccctgctggt ttttctcata cagacgctgc gcctgcttga
     8521 gtttcttgtt taccagcact tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg
     8581 gcgcaacctg gctgcggaac atgcggatct catacagctc gttattgatc gtcgcctgaa
     8641 taatcccctg atgctgttcc gccatggtgg tgatgttttg cagattcaaa tgcggcagca
     8701 aatgatcggc aattttgtta cttatgactg tgcggtttga ttcctgatcg tgaaccagca
     8761 ggccgagcgg cagcagtgag actatctctt cattaattgc ccgtaaaatg cgcaattcgt
     8821 tattgaccgc cgtgctgggg acgctttctg tactgcggct ggagaaatgg cggaatgtgg
     8881 tatagccaaa taacgccagc gccagcaaac cgatgttcag cagcagtggc agcagaatgt
     8941 tttgcaacgt atccagcagt aaggtgccgt aaggaacctg ccagaccagg cgcatatcgg
     9001 tagagttgag tgccgaggag atttcaatct tcgtactgtt aaagtggata ctgacgctat
     9061 ccgtcccttc tttctcattt tcattgtttc ccgttgccgt cgcgtctggc tcaaggcgga
     9121 aactgtccag cggcataccc ggtgggatca aatcattaat cggcagatca aaagccacga
     9181 ccgttgccag atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct
     9241 gccaggccag gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt
     9301 gcaacatctc tgcacgacgt gaatcaacaa tgtcgctgac ggtcgattct ttaaatccgg
     9361 aggtgagatc tttgaggggc agggttgaga tcagcaccag actgttatcc tgaccattca
     9421 ggtaatacat cgaccacggt acattttctg cgccccacaa tgtgtccaga taggtggaca
     9481 tccgttgagt catctcaagc gttgagttgt cgtgagagcc aaagattaac gcttccgttt
     9541 tgcggcgtgg tttctccaga tagtagacat cctgtttcag gcgcgtctct tgtaaacctt
     9601 cgccggagga gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac
     9661 gccaggtatc gacgcgcttt tggattgcgt gggtaatgtc gacaatctgg taacttttat
     9721 ctttcagcca ggcgttaacg gcgctttgta ccattacacc catcgtcacc agtaacacaa
     9781 tgatcaacag taaaaagaag cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg
     9841 ccgttgtctc tttctgacgc attcgtgttt atgacctgtt aaaacttcgc gaagtgatag
     9901 ggcagtataa agggtacagt gtgaaattcc agactcttac ctgattgatg attctctatt
     9961 ctccagtgat atttatcatc cggcattggg ggaaatagat gtacaagcgc cattttgctg
    10021 tacaaataac ctacaaaaag cccaacgaaa ttaattgcaa ataaacgaag attgctggaa
    10081 attatgctga tgttaattat ttgtgaaata gttaacaagc gttatagttt ttctgtggta
    10141 gcacagaata atgaaaagtg tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg
    10201 ttgaaatagg ggtaaacaga cattcagaaa tgaatgacgg taataaataa agttaatgat
    10261 gatatcggga gatattctag ttgcgagtga agg
//
LOCUS       DAPQZD010000168        10147 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPQZD010000168 DAPQZD010000000
VERSION     DAPQZD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.170"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            69..1463
                     /gene="galP"
                     /locus_tag="RO454_004105"
     CDS             69..1463
                     /gene="galP"
                     /locus_tag="RO454_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDY0935854.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1540..2037
                     /locus_tag="RO454_004106"
     CDS             1540..2037
                     /locus_tag="RO454_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDY0935855.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLIAK"
     gene            2132..2839
                     /gene="endA"
                     /locus_tag="RO454_004107"
     CDS             2132..2839
                     /gene="endA"
                     /locus_tag="RO454_004107"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDY0935856.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2919..3650
                     /gene="rsmE"
                     /locus_tag="RO454_004108"
     CDS             2919..3650
                     /gene="rsmE"
                     /locus_tag="RO454_004108"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDY0935857.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3663..4613
                     /gene="gshB"
                     /locus_tag="RO454_004109"
     CDS             3663..4613
                     /gene="gshB"
                     /locus_tag="RO454_004109"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDY0935858.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQVGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4722..5285
                     /locus_tag="RO454_004110"
     CDS             4722..5285
                     /locus_tag="RO454_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDY0935859.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5285..5701
                     /gene="ruvX"
                     /locus_tag="RO454_004111"
     CDS             5285..5701
                     /gene="ruvX"
                     /locus_tag="RO454_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDY0935860.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5816..6796)
                     /locus_tag="RO454_004112"
     CDS             complement(5816..6796)
                     /locus_tag="RO454_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDY0935861.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6814..7518
                     /gene="yggS"
                     /locus_tag="RO454_004113"
     CDS             6814..7518
                     /gene="yggS"
                     /locus_tag="RO454_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDY0935862.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7536..8102
                     /gene="yggT"
                     /locus_tag="RO454_004114"
     CDS             7536..8102
                     /gene="yggT"
                     /locus_tag="RO454_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDY0935863.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8099..8389
                     /locus_tag="RO454_004115"
     CDS             8099..8389
                     /locus_tag="RO454_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDY0935864.1"
                     /translation="MSAVTVNDGGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8397..8990
                     /locus_tag="RO454_004116"
     CDS             8397..8990
                     /locus_tag="RO454_004116"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDY0935865.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            8983..10119
                     /gene="hemW"
                     /locus_tag="RO454_004117"
     CDS             8983..10119
                     /gene="hemW"
                     /locus_tag="RO454_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDY0935866.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2379 a         2684 c         2688 g         2396 t
ORIGIN      
        1 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata accatattgg
       61 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
      121 tctgcttcct tgctgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
      181 gcgcactgcc gtttattgca gatgaattcc agattacttc gcacacgcaa gaatgggtcg
      241 taagctccat gatgttcggt gcggcagtcg gtgcggtggg cagcggctgg ctctccttta
      301 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
      361 tctctgcggc tgcgccaaac gttgaagtac tgattctttc ccgcgttctg ctggggctgg
      421 cggtgggtgt ggcttcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
      481 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tattgggatc ctcggtgctt
      541 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
      601 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
      661 ttgctgccaa acgccgtttt gttgatgccg aacgcgtgct gctgcgcctg cgtgacacca
      721 gcgcggaagc gaaacgtgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
      781 gctgggcgct gtttaaagag aacagcaact tccgccgcgc ggtgttcctt ggcgtactgt
      841 tgcaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
      901 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacggtg attgtcggcc
      961 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     1021 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     1081 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     1141 ttattgttgg ctttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     1201 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     1261 acatgatcgt tggcgcaacc ttcctgacca tgctcaacac gctgggtaac gccaacacct
     1321 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     1381 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     1441 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccacc
     1501 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     1561 atcgccatcc aacaggccgt tatgcgtcgc ctgcgggaaa aactcgccca ggccaacctg
     1621 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     1681 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     1741 agtgaagcct ttattgaaga agtggtgccg cacgaactgg cacatttgct ggtatggaaa
     1801 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     1861 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     1921 ccctaccgct gcaagtgcca ggagcatcag cttaccgtac gccgccataa tcgcgtagtt
     1981 cgtggcgagg ccgtctatcg ctgtgttcac tgcggtgaac agctgattgc aaagtaacca
     2041 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     2101 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     2161 ggtactgagc gcagcatttt ccggcccggc gttggccgaa ggtatcaata gtttttctca
     2221 ggcgaaagcc gcggcggtaa aagtccacgc tgacgcgccc ggtacgtttt attgcggatg
     2281 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     2341 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     2401 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     2461 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     2521 tggcgatcgc ggcaacttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     2581 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     2641 tgccattgcg cgcacctact tctatatgcg tgaccaatac aacctgacac tctctcgcca
     2701 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     2761 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     2821 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     2881 cgaacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     2941 atcctgaacc actgaccagc cattctcata tcgcgctttg cgaagatgct gccaaccata
     3001 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggcagcaacc
     3061 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgttgg
     3121 aaggacagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     3181 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     3241 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     3301 tccagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     3361 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     3421 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccgtta ccggttgaac
     3481 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     3541 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     3601 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     3661 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     3721 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     3781 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     3841 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     3901 tcgacgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     3961 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     4021 gcgactgtaa cgagaaactg tttactgcct ggttctctga cttaacgcca gaaacgctgg
     4081 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     4141 agccactgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     4201 tcggcgtgat tgccgaaacc ctgactgagc atggcactcg ctactgcatg gcgcaaaatt
     4261 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtagatggc gagccggtac
     4321 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     4381 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt caggtcgggc
     4441 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     4501 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     4561 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     4621 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     4681 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     4741 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     4801 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     4861 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     4921 gtctggataa accggttatg ctcggcggcc ctctggctga agatcgcggg tttattttgc
     4981 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     5041 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     5101 ctctgggcta tgcctcctgg gagaaaggtc aactggaaca agaaattctc gataacgcgt
     5161 ggctaacggc cccggcggat ctgaatattc tgttcaaaac accgattgcc gaccgctggc
     5221 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcagggcacg
     5281 cctgatgagt ggaaccttac tcgccttcga cttcggtacc aaaagcattg gtgtagcggt
     5341 cggccaacgc attaccggca ccgctcgccc attgcctgca attaaagcac aggacggtac
     5401 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagccggacg aaatcatcgt
     5461 cggtttgccg ctgaatatgg acggcaccga gcaaccattg actgccagag cgcgtaaatt
     5521 tgccaaccgt attcatggcc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     5581 caccgtggag gcacgttccg gtctgtttga acagggcggc tatcgggcgc tcaacaaagg
     5641 caaagttgac tctgcctctg cggttatcat tctcgaaagt tatttcgagc agggatatta
     5701 aggcgattta cgtcatcgct ccttattgcc ggatgcggcg tgaatgacta cgtttgtagg
     5761 cctgataagc gtagcgcatc aggcatattt actaaaataa tcaatcactt tagcctcaca
     5821 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
     5881 gcccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
     5941 ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt ttatccactt
     6001 ccagcttttg tgacaacact gcccgtaaac tccccgccag ttgattacgt acgggatctt
     6061 tttcctgcgc cggaaatgaa tccaccagcc gctcaactgc ctgcgccgca ccacgcgtat
     6121 gtaatgttgc cagcaccaaa tgcccggttt ctgccgccgt cagtgccaga cggattgtct
     6181 cgctgtcacg cagctctccg agcaatatca catcgggatc ttcccgcaat gcggcacgca
     6241 atcccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
     6301 ggctggcata gagatattca acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
     6361 gattgagata gccaaccatc gccgccagcg tggtagattt gccgctcccc gtcgcccccg
     6421 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggcgcaccaa
     6481 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
     6541 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacaca gcaaaatcca
     6601 actgaccatt ctccagcaat attgtccgct gatcgtcatc cagccactcc cgcagtagct
     6661 cttcgacgtc cggcgcatca aacggcgcag cttccattcg cccgcgaata cgccatcgtg
     6721 cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa
     6781 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
     6841 caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt
     6901 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
     6961 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
     7021 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
     7081 agccgcctgg tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
     7141 acccgcctca acgatcagcg cccggcagaa cttccccctc ttaacgtact gattcaaatt
     7201 aatattagtg acgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
     7261 gctgcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcacct
     7321 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
     7381 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
     7441 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
     7501 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
     7561 caacggtcat tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc
     7621 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtaacgcag ccaattatcg
     7681 ggccactgcg ccgcgttatt ccggcaatgg ggcccattga cagcgcctca ctgctggttg
     7741 cgtacattct cagtttcatc aaagccatcg tgctgtttaa agtggtgacc ttcctgccaa
     7801 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcctgctg attttctggg
     7861 tcctgctggt gatggcgatt atgagctggg taagccaggg gcgtagcccg attgaatacg
     7921 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcctgcta ccggcaatgg
     7981 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg
     8041 tcgcagaagt cttgcaggcg accggaaata tgctgctgcc ggggctgtgg atggcgttat
     8101 gagtgccgta acagtgaatg atggcggtct ggttttacgg ctctatattc agccgaaagc
     8161 cagccgtgat tctattgtcg gtttacatgg cgacgaggta aaagtcgcca ttaccgcgcc
     8221 gccggttgac ggccaggcca acagccatct ggtgaagttt ctcggtaagc aattccgggt
     8281 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
     8341 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
     8401 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta
     8461 gcgacttcgg tcttgatatc gtggcccaaa cagacctcgg tgtagattcc gctgaagaaa
     8521 ctggcctgac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
     8581 gtttaccggc aattgccgac gactccggtc tggcggtaga tgtacttggc ggtgcaccgg
     8641 ggatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
     8701 tgctggaaac aatgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc
     8761 tggtatatct gcgtcacgcg gaagatccca ctccgctggt gtgccacggt agctggccgg
     8821 gcgtgattac tcgtgaacca gcgggcactg gtggctttgg ttatgatcca atcttctttg
     8881 ttccttccga agggaaaacc gctgccgaac tgacccgcga agaaaagagc gccatttccc
     8941 accgtggtca ggcattgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
     9001 ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
     9061 tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct gcttaacgat
     9121 ctggacaacg atgtggctta cgctcagggc cgtgaagtgc agacaatctt tattggcggt
     9181 ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg
     9241 cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg cacggtagaa
     9301 gcggatcgct ttgtcgatta tcagcgtgct ggtgtgaacc gcatctctat tggtgtgcag
     9361 agttttagcg aagaaaagct gaaacgactc gggcgtattc atggcccgca agaagcgaaa
     9421 cgagcggcaa agctggcgag cggtttaggg ttacgtagtt ttaaccttga tttgatgcat
     9481 gggctgccgg atcaatcact ggaagaggcg ctaggcgatc tgcgccaggc tattgaactg
     9541 aatccgccac atctttcctg gtatcaactg accatcgaac ctaatacgct gtttggctcg
     9601 cgccctcctg tactgccgga cgatgacgcg ctgtgggata ttttcgaaca ggggcatcag
     9661 ttattaaccg cagcgggcta tcagcagtat gaaacatccg cttacgccaa acccggttat
     9721 cagtgccagc acaatctcaa ctactggcga tttggcgact acatcggtat tggctgcggc
     9781 gcgcacggca aagtgacctt cccggatggg cgcattctgc gtaccaccaa aacgcgtcat
     9841 ccgcgtggtt ttatgcaggg aagatatctg gaaagccagc gtgatgtcga agctgcagat
     9901 aaaccgtttg agttctttat gaatcgcttc cgtctgctgg aagccgcgcc gcgcgtggag
     9961 tttatccaat atactggcct ttctgaagaa gttattcgct ctcagttaga cgaggccatc
    10021 gctcaaggtt atctcacaga atgtgcggat tactggcaga taaccgagca tggtaaatta
    10081 ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg cctgcgggcg
    10141 cattaat
//
LOCUS       DAPQZD010000169        10004 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPQZD010000169 DAPQZD010000000
VERSION     DAPQZD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.171"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            162..977
                     /locus_tag="RO454_004118"
     CDS             162..977
                     /locus_tag="RO454_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDY0935867.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            1324..3210
                     /locus_tag="RO454_004119"
     CDS             1324..3210
                     /locus_tag="RO454_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDY0935868.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            3251..3778
                     /locus_tag="RO454_004120"
     CDS             3251..3778
                     /locus_tag="RO454_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDY0935869.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            3882..5261
                     /locus_tag="RO454_004121"
     CDS             3882..5261
                     /locus_tag="RO454_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HDY0935870.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            5264..6547
                     /locus_tag="RO454_004122"
     CDS             5264..6547
                     /locus_tag="RO454_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0935871.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            6537..7667
                     /locus_tag="RO454_004123"
     CDS             6537..7667
                     /locus_tag="RO454_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0935872.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALSAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKMVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            7672..8367
                     /locus_tag="RO454_004124"
     CDS             7672..8367
                     /locus_tag="RO454_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0935873.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            8354..8839
                     /locus_tag="RO454_004125"
     CDS             8354..8839
                     /locus_tag="RO454_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDY0935874.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAVFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPPELEATFARLAA
                     R"
     gene            8864..9349
                     /locus_tag="RO454_004126"
     CDS             8864..9349
                     /locus_tag="RO454_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935875.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            9471..>10004
                     /locus_tag="RO454_004127"
     CDS             9471..>10004
                     /locus_tag="RO454_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HDY0935876.1"
                     /translation="MKKLILAAAVAGAVLLSSAAQAQTTPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNQWSEWDVPGGQLTTKGGVLEIYMGHYMREWLAELGMVTSGECP
                     TPDTVYTYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SQKAVQAMEKERSQMQLD"
BASE COUNT         2207 a         2481 c         3007 g         2309 t
ORIGIN      
        1 cattttttaa cccattgtaa ggatgtgtaa aacgagcgga gagcgtgttt tctgttattt
       61 ttgctgcttt tttaccgacg ccagttatta ttttttaccg ctgcctgacg ttgcaaacct
      121 gaattgataa tggcatatct gttttaattg aatgaaagtc tgtgatacgt tcaggcgatt
      181 ttttcgattt ccattttgac agggataaca attcaatgcg caagggtaaa attattgttg
      241 catcaatgat ggtgctttgt gtccttattt ttgttgcggc ggcggcatgg tttatgctgt
      301 tccagggaga aatgacggag ccgacagagg aggaaaaaca ggcggcaatt ttacacgctg
      361 cgggcctgat gaaatcagag acccaggata aaaaatcagt cgaaacgctc tatcatcgtt
      421 atattattca acgccatgtt aatttagatt ccggcgaact ggtggctggc agcagcgcgg
      481 acaccgcgcg gcagaagtgt gaaaaacttg cccctgaacg cgacccagcg caggttcgtc
      541 agcgctgtac ggtggcggat gtgttctttg tgaaagataa aaataatgaa attcagcagg
      601 ttatcattcc agttaccggt aagggtgcga aatccatgat gcatgcgttt cttgctctgg
      661 ggctggacgg tcgtacggtg agaaatctgt attattatca gcaacgggaa acgccgtttc
      721 tgggggctag ggtggaagac gccaactggc gcaaacagtg gccagggaaa cgattattag
      781 ataacagcgg acatcctgca ttgaaaatag tacaggataa acctgaacat gccgatgaat
      841 ataccgttga cggtatttcc ggtgcgacgt tgacatcaac cggcgttgag aaaagtatta
      901 actactggat ggggccgcag ggatatggtc aattcttaca gcgtcttgcc agcgatcgaa
      961 ataaccttaa tctttgaata ttgctgtacg caataacgcg ttttatttct gcctgccgat
     1021 aatgaataat atcactgtcg gcagggggat ttattcctct gttaccgcgc ctgccttgcc
     1081 gttaattcca tgagaaaaac agggagataa cgtccctttt atcaacgcat aatgtgtgtg
     1141 ttcatcacga tttgatgaaa taagtttaca taacttgtcg tctgctgaca tttttatgat
     1201 ccaggtcatg tataacgcga caaaaagata tgataataat tatcatttgt attctttggc
     1261 ggtgtgcacg tgcgtaactt ccgtaactct ttgttggtta ctctctttgg cttgtttata
     1321 agcatgagtg tctgggcgtc gacgaattat gcgcctttga ttgaagatat cgagcaacgt
     1381 cttgataaaa cagcggaact ttatcagcaa cagcatgccg acgaggcgcg ccgcacggta
     1441 caaatggcct attttgaggt gttcgagaac ctggaagggc cgatccgcat caacatttcc
     1501 gcgcgcaaaa gttacgagat ggagagcgca ttcggcgaga tccgtcgaat gattggcgag
     1561 aaaaaaccgc tcgctgacgt ccaggcgcgc attgactggc tgaaagccgc actgcgcgaa
     1621 gtggaaccgg tgctggatgg cggacatcgt ctggtggccg aagagcagca caacgcgctg
     1681 tccagggacg acattgccgt tcactggcag gagagtttca ggacgatcga cgatctgctg
     1741 gcgcaggcgg tcactgaata tcaggccggg aattacagcg ttgccagtca gcacgttcaa
     1801 caggcgcact atcagggctt taaaaactct gagatggaga tgtccctcag acaaaaccgt
     1861 tctgcaaaag atgccgcctc cattaaccaa caattctcat ccctgattgc gctgaccgcg
     1921 caacctgacc atctcaacga tgtctcctat caggtcacta cgctgctaca ggatatcgag
     1981 gatatcttac ccggcttgcc aaccacccgt gacgatcagc aggttgccgc agcgcaaagc
     2041 gcagacaaca ttccggcggc ggacgaggga aataaccgca ccgactgggc tgaggtgacg
     2101 acgggaatta accagagcat tcaggacgcg atagcgcgct atcagggcgg cgatgcgaag
     2161 aatgcgattc tggatatcca ggatacctac ttcgaccgct tcgaagccag cggcatggaa
     2221 aacaaaatcg gctctcgcga ttcggcattt aaaaccacgc tggaggcgta ttttacccgc
     2281 ctggtcagtc tgatgaaggc cggtcagccg gttgagcgcc tgaatgcaga ggccagcgcg
     2341 ctgacgcagg atctgcaaaa agccgtgacg atgctgggcg aaggcgaaga aacccagtgg
     2401 agtctgctgc tctatagtct gatgatcatc gtgcgcgagg ggctggaagc gctgctgatt
     2461 gttgcggcaa tcgtggccta catggtgaaa aacaaccatc aggataagct gccgcttatt
     2521 cgtcagtcgg taattgtggc gctgatcgcc agcgtcatca ccgccgctat cttccagatg
     2581 ctgtttacta actccggcgc cagccgtgaa ctgctggaag gcataacgat gctgattgcc
     2641 gtcgtgatgc tgttctttat gagttactgg ctgctgtcca aagtggaagc gcgccactgg
     2701 aaagcctggc tggaaggcaa actgtctcat tccctgtcgc gaggctccct ggtcggacta
     2761 tggctgacca gcttcctggc ggtgtaccgc gaaggggcgg aaacggtgct gttctattac
     2821 gctctgattg gcgatgcaaa cacggtatcc ggccatatgg cgattggcgc cggttttgtg
     2881 attggctgcg tggtgctgct gctcgcctgg ctggtgatgc gttactcagt ggtgcgcctg
     2941 ccgctgaaac ccttctttat gtttaccggc agtttcatgt atctgatggc cttcgtcttt
     3001 gcgggcaaag gcgttctgga actggtggaa ggcaaactgt tccagcctac gttgattaac
     3061 ggcttccctg aaatcagctg gctggggatt tatccctacg tggaaacgtt gttaccgcag
     3121 gtcgtattgc tgctcgcggc gctggtggcg ctgtgggtga tgcggcgcaa aagcgcggtt
     3181 cccggggaga cgataaaaaa taacccataa tcgttgtata gccgttgttt tagacgagag
     3241 gatacgtttg atgaccatga agaaaaccct gattgccagc gcagtgatgg ccagcatttt
     3301 cattgcgcca gccgcgtttg cttttaaaga gtacccggca ggcgagcctg tcaccatgaa
     3361 tgaaatggag ctggcggccg tttacctgca accgattgat atggaaccgc gcggcatggg
     3421 cttaccggca gcaaaagccg atgttcacct tgaagcggat atccacgctg tagaaggcaa
     3481 taaaaacggc tttggcgcag gggaatggat cccgtacctg accatcagct acaccctggt
     3541 caacaacgac acgggcgaaa aacaggaagg caccttcatg ccgatggttg ccagcgatgg
     3601 cccgcactat ggcgcgaaca tcaaaatgat gggcgtgggc aactataaag tgacctacca
     3661 cattgaaccg ccgtcaaaag ccgggatgca tcgccatact gacagcgaaa caggcgtagg
     3721 ccgctggtgg aaaccgtttg atgtgagcta cgagtttaaa tatgtcggtc tgaactaaaa
     3781 ccgactaacc aacgcgccag gaggattccc ccttctggcg cgttggtcag accgatcata
     3841 tgcccgtcgc ttaattcaac ccgccgtaca gagtcgaagt tatgagttac tttttcgtca
     3901 cgacgctaca ggtctttttc tgcattgcgc ttctttccgg tgttctctgg agtcgaaacg
     3961 atcctccctc tttacgtccg ctgacctgga cgctgctgac cgggctgatt gccggcgtgc
     4021 tggcgggact gtttattcac ggcagccaac cggtacaact gctgctggtt ggggcggagg
     4081 tcatggtatc gctgctgttt gtcctcagtt tctggtgggc ttcaacgcgt atccgctatc
     4141 tgtggcaggg gattctgatt ttcggcgctg cccgccattg ggcgctggac cctaatctgg
     4201 gcgggctgac cagtacgcat gtgctgaata ctgacctgct gttaaacctg acggccgtcg
     4261 ttctcgcttt tgcgatcctc tgtctggcgg gcgtactctg cgcgatgctg ctgcggcgta
     4321 ttcgcggcct gtactggccg ttaacgctga ttctgctggt gatgatctgg ctgccgctga
     4381 gcggcaacct gctgttgttg ctgatgaaat tacaggttgt tccgctggga aaatcactgc
     4441 tgagcttcgt ggcgaaagtc acaaataaca cggcgctgta taactgggcg ggagccgcgc
     4501 tgctgctggc cctggcgctg tgctggcttc cggcgctgtt gcgggctttt cgacagacgc
     4561 gggaaaccga agaaccgatt gctcaccgtc tggcgctggc gcagcgtcgc aacgctttgc
     4621 ggctgtggtt ggtcacaatc ggctgcgcgg tggtcgttat cgcaggccaa ctgtggtggg
     4681 ataaagtggc ctcgcagccg ccgcaactct ctgaagccgt gcccgtaacg ctggggagcg
     4741 atggcatggt gcgcctgccg gttgaacagc ttcgtgacgg taaactgcat cgtttcgtct
     4801 gggtggcgga cgatggcaaa gcggtgcgct tcttcgtgat taaccgctat cccgacaagc
     4861 tgcgttttgg cgtggtgttc gacgcctgcc tgctgtgcgg cgatcagggt tatgtcatgg
     4921 agggcaatca ggttatctgc gtcgcctgcg gagtgcatat ttttattccg tctatcggca
     4981 aggccggtgg ctgtaacccg gtgccgattg aaaactggca caatgatgaa aaagagctgg
     5041 tgatccccgg taaagaactg gcgacagggg tgaactactt ctcgacggtc atgactatta
     5101 aagtgaccga tccggtcgat ggttcaacgt tgaccaatac ctcagccgat tacaaataca
     5161 gctatggcgg caagacctgg ttcttctcgt cagaggctaa ctatgaacgc ttccgtgaaa
     5221 cgccggagca gtttgttcct gccgacatga gggaggaata atcatgctgt ggcggatgtt
     5281 acgacaatcc tgggggcgca atttacgccg caaagtgctg gcgataatca ccgtttttct
     5341 ggcctccagc ctgatctcgg cgctgctggc ggtgtccatt gatatcggcg acaaaatgtc
     5401 cagagagctg aagtcttatg gtgcgaacat tttgattgaa cccgccggac aggctgcgct
     5461 gcccgcgctg tttagtgaga gcagtaatcc gctttccggt caggattttc ttgatgaagc
     5521 cgagctgccg aacatcaaag atatcttctg gcgaaacaac attgtcgggt ttgcgccgat
     5581 gctcggcggc gaagccagcg tggagggaga accagtgcgg attctgggca ctttcttcag
     5641 tcagccagtg gatattcctg atgaagaggg atatgagacg gggcagaaaa cggtcagccc
     5701 atactggcag gtgaccggcg actggccgca ggaacccgca ggcgcagagc cgcagacctt
     5761 agtcgggcac gcgctggcgc ggcaaatggg ctggaaaccc ggtgataagc tgacattacg
     5821 taccgaaggc gaagcagtgc aggtcaccgt cagcggtatc ctctccagcg gcggcgatga
     5881 agataaccaa ctggtgatgc cgctaagcac ggtacagcac ctgctggggc tccccggaaa
     5941 agtgcaggcg attcgcgtct cggcgctgac ggtgccggaa aacgaactgt cccgccgcgc
     6001 gcgggaaaac ctggacgcgc tcaatgccga agagtacgac ctctggtatt gcacggccta
     6061 tgtgtcgtcg atcgcgcatc agctggaaga ggcgatttcc ggtgcggaag tgcgcccggt
     6121 atggcaggtc gcggcgtccg aaggggtggt gatcgataaa attcagttgc tgatggcggt
     6181 cgttaccgtc gcggcgctgg tggcttccgc aatggggatc gcctcgctga tgaccagcac
     6241 cattatggaa cgcgccaaag agatcggttt gatgaaagcg ctgggtgcgc gacagtggca
     6301 aattatgctg ctgttctacc tggaagcggc gtccagcggg ctggctggcg gtgcgctggg
     6361 atgtattgcc gggtgggggc tggcgaaagc gattggcgtc atgctcttcg acgcgccgct
     6421 taattttgcc tggattgtgg tgccgtgcgt actggtgatt gcggtactga ttgcgttgat
     6481 cggcacctgg ttcccggccc ggcggattgc ccggctgtat cctgtggagg tgctgtatgg
     6541 ccgctaaacg taccatgctc tggctgctgg tgtggcgggc gctgcgtctg cgttttcagc
     6601 gggtcagcgt ggtgtttgcc gcgctgatgg tgggggcgac catcgtgacg gcgctttctg
     6661 ccgtctggtt cgacattaac accaaaatga gcgaagaact gcgcaccttc ggcgcgaatt
     6721 tttacatcgg gccggggcac ggttccagta tgccgcagca ggagttgcag agcattctcg
     6781 atcaggcgcc gcaggggctg gtgcatggcg ccagtccgtg gttgtacggc atggcgcgta
     6841 ccgagctgga aaaagtggtg atggttggcg tgtggttcga gtcgctgcaa aaaatggtgc
     6901 cgtactggca ggtcaccggt agctggattg gcgtcagttt tgacgaccgt aatgcgatga
     6961 tcggcgtcaa actggcggaa cgcctcaacg tgcagccggg cgacagcatc acgctggtgg
     7021 atcacaatca gcgtaagaat ttgcagatta aaggcattgt ggagtctggc gatgcgacgg
     7081 acaacatgct gatcgtcagc ctcgacgtgg cgcaggcctg gctgcatcag ccgggcaaaa
     7141 tcagccacgg cttgctgagc gtcagtaacg atgtcggcca ggtggagaac tatgccagcc
     7201 ggttgcaggc gcagtatcct gacctggaga ttcgtccggt gcgtaaggtc tctgcttccg
     7261 aagggcaggt gctggataaa attaaaggtc tgatgggact ggtctcgatc gtgattctgg
     7321 cgctctcttc gctgtgcgtg aataccacgc tgatggcgat tgtcggcgag cgggcgcgcg
     7381 agtttgcgct gcaaaaagcg cttggttcca gcaacggcga tatcgtgcgg caaattctgc
     7441 tggaaaccag catcatcgcg ctggcggcgg tggcgtgcgg ctgggtgctg ggatatttgc
     7501 tggcgcaact gttgggatta acggtgttta atgccgccat atcactgcgt ctgccggtgt
     7561 tgcccattac tctggtgttg tctttactgg tcgccattct ggcggcgatt gttccggtgc
     7621 gacgcgcggt aagcgtcgag cctgctaaag tgctgaaagg agagtagcgc catgtccgtt
     7681 acgcatatcc ctcaggtggc gatcgaaacc cgccacttat acaaacggtt cggtgacgtt
     7741 accgccctgg atgacattaa tctgcgcatt gcgcagggtg aatttgtcgc cattatgggc
     7801 gcttctggtt ctggtaaaac cacgctgatg aatattctga cgtgccttga tacggcgacg
     7861 gaagggcagg tgtttcttga tggcaccgat gccgccgcgc tggatgaaga ggggcgccgt
     7921 cgctttcgcg ccgagaaaat cggcctggta tttcagcagt tccatttgat cccgttctta
     7981 actgcgctgg aaaacatcat gctcgcccag cattaccaca gcgtggtgga tgaagcggcg
     8041 gcgaggaagg tgctggaaca ggtagggctt gggcatcgtg tgacgcacct gccgagccag
     8101 ctttccggtg gtgagcagca gcgtgtgtgt atcgcccggg cgctggtcaa cgaaccgccg
     8161 gttattttcg cggatgaacc gacgggcaac ctggatgaag agaatgagca gcgggtgctg
     8221 gatctgctca ccgatctgca tcgtcagggg cgcacgattg tgatggtgac gcacaaccct
     8281 gcgcttgggc agtttgccga ccgtattctg cggctgcaac acggtaaata tctgggtgag
     8341 gaggccaatc aacatgcgct ggcataatgt gctgttactc gcggcggcga tcctgataag
     8401 cgggtgtaaa gaagaaaagc tggcggtcgg ggcaccggcc ccggcgctgg cggtgtttga
     8461 tctgcaaggc caggagtcag gccttgaacg ctggcaagga aaggcggtgt acctgaattt
     8521 ttggtctgcg ggatgcgggg ggtgcctggc ggaaatggac acgctggaag cgctgagcaa
     8581 aaaatggggc gataaagtgg tggttgtggc ggtcaatacc gaccctgaaa cggtcaggat
     8641 caacgatctg ctggcaaaac atcagataac ctatccggtg gtgcgcgatc agctcaacat
     8701 tacccaggaa cgttatcagg tgatcggcac gccgacctcc gtactgattg actccgaagg
     8761 acgcgttctg gagctacacc agggtatgcg taagccgcct gagttagagg ccacgtttgc
     8821 gcgcctggcg gcgcgatgaa cgacgcgtac ggagagatac ccaatggcat ttatctggaa
     8881 cgacgaaagc cttgcgattc tgcgggaaaa cgcgggcatc ctcacgacag agcagatagc
     8941 gcaactgctt cacactaata ttaccgccgt gcggaatatg gcctatcggc tgaaactgag
     9001 ccttcgggtg acggcgtata atcaccgacg catagcgcag gttcaggcgc tttatgcctc
     9061 tgagacgttg agtctcaaag agatcgcggc gaaaacgggg ctgacggcca gtaccgtgca
     9121 atatatcgtg tacgtgaaat caaaaaataa gccctacgcc accacggaat atgtctcttt
     9181 tgaaacggag aacgccgttc attaccgcgt gcaaaaagaa tttgtcgata cggaacgttc
     9241 cctgctggac aacatctctg ataacacgcg atttcgtgaa ctgtatctga cggacggcac
     9301 tttttattgc gcccgcaata ttaagtatga ggtttttatc tcggagtaac cggaacacgc
     9361 tgacctgccg gttatcttct ggaatctccc gttttgcgaa accgttcccg gaaaaatgtc
     9421 atatttctgt cacactctat gaggattgat aacaacaaga ggttgtaggg atgaaaaaat
     9481 tgatactcgc cgccgctgtg gcaggggctg ttttattgtc gtccgccgcg caagcgcaga
     9541 caacgccgga aggttatcaa ctgcaacaag tgttgatgat gagccgacac aacttgcgcg
     9601 cgccgttggc caataatggc agcgtgctgg agcagtccac gccgaatcag tggtcggagt
     9661 gggatgtgcc cggcgggcag ctcaccacga aaggcggtgt gctggaaatt tatatgggcc
     9721 actacatgcg agaatggctg gcggagctgg ggatggtcac gtcaggcgaa tgcccgacgc
     9781 cggacacggt atatacctat gccaacagtc tgcaacgcac cgttgcgacg gcacaattct
     9841 ttatcaccgg cgctttccct gggtgcgaca ttccggtaca tcatcaggaa aaaatgggca
     9901 cgatggaccc aacgtttaat ccggtgatca ccgatgactc tgcggcgttt agccagaaag
     9961 cggtgcaggc gatggaaaaa gagcgcagcc agatgcagct tgac
//
LOCUS       DAPQZD010000170         9969 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPQZD010000170 DAPQZD010000000
VERSION     DAPQZD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.172"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            66..1667
                     /gene="aceB"
                     /locus_tag="RO454_004128"
     CDS             66..1667
                     /gene="aceB"
                     /locus_tag="RO454_004128"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDY0935877.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1697..3001
                     /gene="aceA"
                     /locus_tag="RO454_004129"
     CDS             1697..3001
                     /gene="aceA"
                     /locus_tag="RO454_004129"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDY0935878.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            3126..4850
                     /gene="aceK"
                     /locus_tag="RO454_004130"
     CDS             3126..4850
                     /gene="aceK"
                     /locus_tag="RO454_004130"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDY0935879.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(4867..5691)
                     /gene="iclR"
                     /locus_tag="RO454_004131"
     CDS             complement(4867..5691)
                     /gene="iclR"
                     /locus_tag="RO454_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDY0935880.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            5892..9575
                     /gene="metH"
                     /locus_tag="RO454_004132"
     CDS             5892..9575
                     /gene="metH"
                     /locus_tag="RO454_004132"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDY0935881.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2411 a         2527 c         2778 g         2253 t
ORIGIN      
        1 aaagcgcaca acgatccttc gttcacagtg gggaagtttt cggatccatg acgaggagct
       61 gcacgatgac tgaacaggca acaacaatcg atgaactggc tttcacaagg ccgtatggcg
      121 agcaggagaa gcaaattctt actgccgaag cggtagaatt tctgactgag ctggtgacgc
      181 attttacgcc acaacgcaat aaacttctgg cagcgcgcat tcagcagcag caggatatcg
      241 ataacggaag gttgcctgat ttcatttcgg aaacagcttc cattcgtgat gctgactgga
      301 aaattcgcgg gattcctgcg gacttagaag atcgtcgcgt agagattacc ggacctgtag
      361 agcgcaagat ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg
      421 aagattcact ggcaccagac tggaacaaag tgatcgatgg gcaaattaac ctgcgcgatg
      481 cagttaacgg caccatcagc tataccaatg aagcaggcaa aatttatcag ctcaagccca
      541 atccagcggt tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa catgtcacct
      601 ggcgtggtga ggcaatcccc ggtagcctgt ttgattttgc gctctatttc ttccacaact
      661 atcaggcact gttggcaaag ggcagcggtc cctatttcta tctgccgaaa acccagtcct
      721 ggcaggaagc ggcctggtgg agcgaagtct tcagctatgc agaagatcgc tttaatctgc
      781 cgcgcggcac catcaaggcg acgttgctga ttgaaacgct gcccgccgtg ttccagatgg
      841 atgaaatcct tcacgcgctg cgtgaccata ttgttggtct gaactgcggt cgttgggatt
      901 acatcttcag ttatatcaaa acattgaaaa actatcccga tcgcgtcctg ccagacagac
      961 aggcagtgac gatggataaa cccttcctga atgcttactc acgcctgttg attaaaacct
     1021 gccataaacg cggtgctttt gcgatgggcg gcatggcggc gtttattccg agcaaagatg
     1081 aagagcgcaa taaccaggtg ctcgacaaag taaaagcgga taaatcgctg gaagccaata
     1141 acggtcacga tggcacatgg atcgctcacc cgggccttgc tgatacggca atggcggtat
     1201 tcaacgatat tctcggctcc cgcaaaaacc agcttgaggt gatgcgcgaa caagacgcgc
     1261 cgattactgc cgatcagctg ctggcacctt gtgatggtga acgcaccgaa gaaggtatgc
     1321 gtgccaacat tcgcgtggct gtgcagtaca tcgaagcgtg gatctctggc aatggctgtg
     1381 tgccgattta tggcctgatg gaagatgcgg cgacggctga aatttcccgt acctcaatct
     1441 ggcagtggat ccatcatcaa aaaacgttga gcaatggcaa accggtgact aaagccttgt
     1501 tccgccagat gctgggcgaa gagatgaaag tcattgccag cgaactgggc gaagaacgtt
     1561 tctcccaggg gcgttttgac gatgccgcac gcttgatgga acagatcacc acttccgatg
     1621 agttaattga tttcctgacc ctgccaggct accgcctgtt agcgtaaacc accacataac
     1681 tatggagcat ctgcacatga aaacccgtac acaacaaatt gaagaattac agaaagagtg
     1741 gactcaaccg cgttgggaag gcattactcg cccgtacagc gcggaagatg tggtgaaatt
     1801 acgcggttca gtcaatccgg aatgcaccct ggcgcaactg ggcgcggcga aaatgtggcg
     1861 tctgctgcac ggcgagtcga aaaaaggcta catcaacagc ctcggtgcac tgactggcgg
     1921 tcaggcgttg caacaggcga aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt
     1981 agcggcggac gctaacctgg cggccagcat gtatccggat cagtcgctct atccggcaaa
     2041 ctcggtgcca gctgtggtag agcggatcaa caatactttc cgccgtgcgg atcagatcca
     2101 atggtccgcg ggcattgagc cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt
     2161 cgccgatgcg gaggccggtt ttggcggtgt actgaatgcc tttgaactga tgaaagcgat
     2221 gattgaagcc ggtgcagcgg cagttcactt cgaagatcag ctggcatcag tgaagaaatg
     2281 tggtcacatg ggcggcaaag ttttggtgcc aactcaggaa gctattcaga aactggtcgc
     2341 ggcgcgtctg gcagctgatg tgacgggcgt tccaaccctg ctggttgctc gtaccgatgc
     2401 cgatgcggcg gatctgatta cctccgattg cgacccatat gacagcgaat ttattactgg
     2461 cgagcgtacc agtgaaggct tcttccgtac tcatgcgggt attgagcaag cgatcagccg
     2521 tggcctggcg tatgcgccat atgctgacct ggtatggtgt gaaacctcca cgccggatct
     2581 ggaactggcg cgtcgctttg cacaagccat ccacgcgaaa tatccgggca aactgctggc
     2641 ttataactgc tcgccgtcgt tcaactggca gaaaaacctc gacgacaaaa ctattgccag
     2701 cttccagcag cagctgtcgg atatgggcta caagttccag ttcatcaccc tggcaggtat
     2761 ccacagcatg tggttcaaca tgtttgacct ggcaaacgcc tatgcccagg gtgagggtat
     2821 gaagcactat gttgagaaag tgcagcagcc ggaatttgcc gccgcgaaag atggctatac
     2881 cttcgtatct caccagcagg aagtggggac aggttatttc gataaagtga cgaccattat
     2941 tcagggcggc acgtcttcag tcaccgcgct gaccggctcc accgaagaat cgcagttcta
     3001 agcaactgat aaagttgatg attgtaggcc ggataaggcg ttcacgccgc atccggcaat
     3061 caacgcctga tgcgacgatt tgcgcgtctt atccggcaat tctctgctcc tgatgagggc
     3121 gctaaatgcc gcgtggcctg gaattactga ttgcacaaac cattctgcaa ggcttcgacg
     3181 ctcagtatgg tcgattcctc gaagtgactt ccggggcgca gcagcgtttc gaacaagccg
     3241 actggcacgc tgtccagcaa gcgatgaaaa accgtatcca tctttacgat catcacgtgg
     3301 gtctggttgt ggagcaactg cgctgcatta ctaacggcca aagcacggac gcggcatttt
     3361 tactgcgcgt taaagagcat tacacccgac tgttgccgga ttacccgcgc ttcgagattg
     3421 cggagagctt ttttaactcc gtgtactgtc ggttatttga ccaccgctcg cttactcccg
     3481 agcggctttt tatctttagc tcccagccag agcgccgttt tcgtaccatt ccccgcccgc
     3541 tggcgaaaga ctttcacccc gatcacggct gggaatctct gctgatgcgc gttatcagcg
     3601 acctgccgtt gcgtctgcgc tggcagaata aaagccgtga cattcattac atcgttcgcc
     3661 atctaacgga aacgctgggg acagacaacc tcgcggaaag tcatttacag gtggcgaacg
     3721 aactgtttta ccgcaataaa gccgcctggc tggtgggcaa actgatcacg ccttccggca
     3781 cattgccatt tttgctgccg atccaccaga cggacgacgg cgagttattt attgatacct
     3841 gtctgacgac gaccgccgaa gcgagcatcg tctttggctt tgcgcgttct tattttatgg
     3901 tttacgcgcc gctgcccgca gcgctggtcg agtggctacg ggaaattctg ccaggtaaaa
     3961 ccaccgctga attgtatatg gctatcggct gccagaagca cgccaaaacc gaaagctacc
     4021 gcgaatatct cgtttatcta cagggctgta atgagcagtt cattgaagcg ccgggtattc
     4081 gtggaatggt gatgttggtg ttcacgctgc cgggctttga ccgggtattt aaagtgatca
     4141 aagacaagtt cgcgccgcag aaagagatgt ctgccgctca cgttcgcgcc tgctaccaac
     4201 tggtgaaaga gcacgatcgc gtgggccgaa tggcggacac ccaggagttt gaaaactttg
     4261 tgctggagaa gcggcatatt tccccggcat taatggcatt actgctccag gaagcagcgg
     4321 aaaaaatcac cgatctcggc gaacaaattg tgattcgcca tctttatatt gagcggcgga
     4381 tggtgccgct caatatctgg ctggaacaag tggaaggtca gcagttgcgc gatgccattg
     4441 aagaatacgg taacgctatt cgccagcttg ccgctgctaa cattttccct ggcgacatgc
     4501 tgtttaaaaa cttcggtgtc acccgtcacg ggcgtgtggt gttttatgat tacgatgaaa
     4561 tttgctacat gacggaagtg aacttccgcg acatcccgcc gccgcgctac ccggaggacg
     4621 aacttgccag cgaaccgtgg tacagcgtct cgccgggcga tgttttcccg gaagagttcc
     4681 gtcactggtt atgtgccgac ccgcgtattg gtccgctatt tgaagagatg cacgccgact
     4741 tgttccgcgc tgattactgg cgcgcactac aaaaccgtat acgtgaaggg catgtggaag
     4801 atgtttatgc gtatcggcgc aggcagcggt ttagcgttcg attcgtataa atcagaggcg
     4861 aaaaagtcag cgtattccac cgtatgccag cgtcacttcc ttcgccgctt taatcaccat
     4921 cgcgccaaac tcggtcacgc ggtcatcggt aatacgtgaa atcggtccgg aaattgaaat
     4981 tgcggcaaac ggttcgcggt gctcatcgaa aatacacgct gcaaggcaac gcagccccag
     5041 cgcatgttcc tcatcgtcaa atgaataacc ccgtttgcgc gtttgggcga gatcttcttt
     5101 taaatgcaca ggagacacca gcgttgcgtg ggtataggca tgtaaccctt tgcggtgcag
     5161 taacttcgtc acctgttctt cgctcagttg ggccagaaag gctttacccg caccggaagc
     5221 gtgcatcggc aatttaccgc cgataggcgc ggacattcgc atcaggtgag tacactgtac
     5281 ctggtcgata ataatcgctt cgtgattgct ttgatcaagc accgccatat tgaccgtttc
     5341 gccagactct tccattagat tgcgcaggat agggtgaaca atcgctaaca aattacggct
     5401 ctggagaaag ctgctgccga ccataaaggc gtgtgcgccg atcgcccagt gacccagttc
     5461 gccgacctga cggacaaagc cctgttgctg catcgtggtt agcaggcggt gggtcgtgga
     5521 attgggtaac ccggcttgct gtgccagttc cgtgagtgcc acactgccat tggattcggc
     5581 aatccactcc agtaatttca ggccacgcgt taaagactga acctgtccag tcgctggtgc
     5641 ggtggcaacg gcgggttttc tgccgcgttt tgcgggaatg ggtgcgacca tgacagtctc
     5701 cttttttctg tatcgtggaa atcattttca tttttattat cagcgaatgc aatagttgct
     5761 gaacagatcc gatgagttaa tgttgaacaa atctcatgtt gcgtggtggt cgcttttacc
     5821 acagatgtgt ttatgccagt atggtttgtt gaatttttat taaatctggg ttaagcgtgt
     5881 cgggagcaag tgtgagcagc aaagtggaac aactgtgtgc gcagttaaat gaacgtattc
     5941 tggtgctgga cggcggtatg ggcaccatga tccagagcta tcgactgaac gaagccgatt
     6001 ttcgtggtga acgctttgcc gactggccat gcgatctcaa aggcaacaac gacctgctgg
     6061 tgctcagcaa accggaagtg atctccgcca tccacaacgc ttactttgaa gcgggtgcgg
     6121 atatcatcga aaccaacacc ttcaactcca cgactattgc gatggcggat taccagatgg
     6181 aatccctgtc ggcggaaatc aactttgcgg cggcgaaact ggcgcgcgcc tgtgccgacg
     6241 agtggactgc ccgcacgccg gagaaaccgc gctacgttgc cggtgttctc ggcccgacca
     6301 accgcacggc gtctatttct ccggacgtca acgatccggc attccgcaac atcacttttg
     6361 accagctggt ggcggcttat cgagaatcca ccaaagcgct ggtggaaggt ggcgcggacc
     6421 tgatcctgat tgaaaccgtt ttcgacaccc ttaacgccaa agcggcggtc tttgcggtga
     6481 aaacggagtt tgaagcgctg ggcgttgaac tgccgattat gatctccggc accatcaccg
     6541 acgcctccgg gcgcacgctc tccgggcaga ccactgaagc gttttacaac tccctgcgcc
     6601 acgccgaagc gctgaccttt ggcctgaact gtgcgctggg gccagacgaa ctgcgccagt
     6661 acgtacagga actgtcgcgt attgcggaat gctatgtcac cgcccacccg aacgccgggc
     6721 tgcccaacgc ctttggtgaa tacgatctcg acgccgacac gatggcaaaa cagatacgtg
     6781 aatgggcaga agcgggcttt ctcaatatcg tcggcggctg ctgcggcacc acgccacaac
     6841 atattgcagc gatgagtcgt gcagtagaag gattagcgcc gcgcaaacta ccggaaattc
     6901 ccgtagcctg ccgtttgtcc ggcctggagc cgctgaacat tggcgaagat agcctgtttg
     6961 tgaacgtggg tgaacgcacc aacgtcaccg gttccgctaa gttcaagcgc ctgatcaaag
     7021 aagagaaata cagcgaggcg ctggatgtgg cgcgtcagca ggtggaaaac ggtgcgcaga
     7081 ttatcgatat caacatggat gaagggatgc tcgatgccga agcggcgatg gtgcgttttc
     7141 tcaatctgat tgccggtgaa ccggatatcg ctcgtgtgcc gataatgatc gactcctcga
     7201 aatgggacgt tattgaaaaa ggtctgaagt gtatccaggg caaaggcatt gttaactcta
     7261 tctcgatgaa agagggcgtc gacgccttta tccatcacgc gaaactgttg cgtcgctacg
     7321 gtgcggcagt agtggtaatg gcttttgacg aacagggcca ggccgatacc cgcgcacgga
     7381 aaatcgagat ttgccgtcgg gcgtacaaaa tcctcaccga agaggttggc ttcccggctg
     7441 aagatatcat cttcgacccg aacatcttcg cggtcgcaac tggcattgaa gagcacaaca
     7501 attacgcaca ggactttatc ggcgcgtgtg aagacatcaa acgtgaactg ccgcacgcac
     7561 tgatttccgg cggcgtttct aacgtgtcgt tctcgtttcg tggtaacgat cctgtgcgcg
     7621 aagccattca cgcggtgttc ctctactacg ctattcgcaa tggcatggat atggggatcg
     7681 tcaacgccgg gcaactggcg atttacgacg acctgcctac cgagctacgc gacgcggtgg
     7741 aggatgtgat cctcaaccgc cgcgacgatg gcaccgagcg tttactcgag cttgccgaga
     7801 aatatcgcgg cagcaaaacc gacgacaccg ctaacaccca gcaggcggag tggcgctcgt
     7861 gggaagtgaa taaacgtctg gaatactcgc tggtcaaagg cattaccgag tttatcgagc
     7921 aggataccga agaagcccgc cagcaggcta cgcgcccgat tgaagtgatt gaaggcccgt
     7981 tgatggacgg catgaacgtg gtcggcgacc tgtttggcga gggcaaaatg ttcctgccgc
     8041 aggtggtgaa atcggcgcgc gtaatgaagc aggcggtggc gtatctcgag ccgtttattg
     8101 aagccagcaa agagcagggt aaaaccaacg gcaagatggt gatcgccaca gtgaagggcg
     8161 atgtccacga catcggtaaa aatatcgttg gcgtggtgct gcaatgtaac aactacgaaa
     8221 ttgtcgatct cggcgtgatg gtgcctgcgg aaaaaattct ccgtaccgct aaagaagtga
     8281 atgctgattt gattggcctt tcggggctga tcacgccgtc gctggacgag atggttaacg
     8341 tggcgaaaga gatggagcgc cagggcttca ccattccgct attgattggc ggcgcgacca
     8401 cctcaaaagc gcacacggcg gtgaaaatcg agcagaacta cagcggcccg acggtgtatg
     8461 tgcagaacgc atcgcgcacc gttggtgtgg tggcggcgct gctttccgac actcagcgcg
     8521 atgatttcgt tgctcgtacc cgcaaggagt acgaaaccgt gcgtattcag cacggacgaa
     8581 agaaaccacg cacaccaccg gtcacgctgg aagcggcgcg tgataacgac ttcgcttttg
     8641 actggcaggc ttacacgccg ccggtggcgc accgtctcgg tgtgcaggaa gtcgaagcca
     8701 gcatcgaaac gctgcgtaat tacatcgact ggacgccgtt ctttatgacc tggtcgctgg
     8761 ccgggaagta tccgcgcatt ctggaagatg aagtggtagg tgttgaggcg cagcggctgt
     8821 ttaaagacgc caacgacatg ctggataaat taagcgccga aaaaatgctg aacccgcgtg
     8881 gtgtggtggg cctgttcccg gcaaaccgtg tgggcgatga cattgaaatc taccgtgacg
     8941 aaacgcgtac ccatgtgatt aatgtcagcc accatctgcg ccaacagacc gaaaaaaccg
     9001 gcttcgctaa ctactgtctc gctgacttcg ttgcgccgaa ggtttccggc aaagcggatt
     9061 acatcggcgc atttgccgtg actggcgggc tggaagagga cgcactggct gatgcctttg
     9121 aagcacagca tgatgattac aacaaaatca tggtgaaagc gcttgccgac cgtctggcgg
     9181 aagcctttgc ggagtatctc catgagcgtg tgcgtaaagt ctactggggc tatgcgccga
     9241 acgagaacct cagcaacgaa gagctgatcc gcgaaaacta ccagggcatc cgtccggcac
     9301 cgggctaccc tgcctgcccg gaacatacgg aaaaagccac aatctgggag ctgctggaag
     9361 tggaaaaaca taccggtatg aaactcacgg aatctttcgc catgtggccg ggtgcatcgg
     9421 tttcgggttg gtacttcagt catccagaca gcaagtatta cgcggtggcg caaattcagc
     9481 gcgatcaggt tgaagactat gcccaccgta aaggaatgag cgttaccgaa gttgagcgtt
     9541 ggttggcacc gaatctgggg tatgatgccg agtaagtgat aacaccgggg tatttgcccc
     9601 ggtttttaat gtcataaata atggattatt gaatatgata aaaattgatg aaatagattt
     9661 tttcgctttc gaaggatgtt cagagtccga agttactgaa ctattaggaa aaaaccaaca
     9721 ggttataatg attggataat taatataaaa caggtgtgga gtgggtattt tatgatggtc
     9781 gttattggtt agattttcgt aaatccaaag agggtataag gtgtatctca tgcagtattc
     9841 tgtatcacga taaaagtaaa gggcgtatcg atgtcttatt tagtgaaggt atagctgaca
     9901 gaggtttaaa aaatacgggt ggagtgttaa tccaaaaacg cccgcaatta taataattcc
     9961 tggtaatat
//
LOCUS       DAPQZD010000171         9901 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPQZD010000171 DAPQZD010000000
VERSION     DAPQZD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.173"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            295..918
                     /gene="rcsA"
                     /locus_tag="RO454_004133"
     CDS             295..918
                     /gene="rcsA"
                     /locus_tag="RO454_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDY0935882.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(962..1150)
                     /gene="dsrB"
                     /locus_tag="RO454_004134"
     CDS             complement(962..1150)
                     /gene="dsrB"
                     /locus_tag="RO454_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDY0935883.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            1313..1540
                     /gene="yodD"
                     /locus_tag="RO454_004135"
     CDS             1313..1540
                     /gene="yodD"
                     /locus_tag="RO454_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDY0935884.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1836..2651
                     /locus_tag="RO454_004136"
     CDS             1836..2651
                     /locus_tag="RO454_004136"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDY0935885.1"
                     /translation="MFSIQQPLLVFSDLDSTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWITATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2648..4342)
                     /gene="yedQ"
                     /locus_tag="RO454_004137"
     CDS             complement(2648..4342)
                     /gene="yedQ"
                     /locus_tag="RO454_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDY0935886.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLKNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4513..4695)
                     /locus_tag="RO454_004138"
     CDS             complement(4513..4695)
                     /locus_tag="RO454_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDY0935887.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4774..5691)
                     /locus_tag="RO454_004139"
     CDS             complement(4774..5691)
                     /locus_tag="RO454_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDY0935888.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5864..6784
                     /gene="yedA"
                     /locus_tag="RO454_004140"
     CDS             5864..6784
                     /gene="yedA"
                     /locus_tag="RO454_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDY0935889.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6773..7243)
                     /gene="vsr"
                     /locus_tag="RO454_004141"
     CDS             complement(6773..7243)
                     /gene="vsr"
                     /locus_tag="RO454_004141"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDY0935890.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7224..8642)
                     /gene="dcm"
                     /locus_tag="RO454_004142"
     CDS             complement(7224..8642)
                     /gene="dcm"
                     /locus_tag="RO454_004142"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDY0935891.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8709..9404)
                     /locus_tag="RO454_004143"
     CDS             complement(8709..9404)
                     /locus_tag="RO454_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDY0935892.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9444..9809)
                     /locus_tag="RO454_004144"
     CDS             complement(9444..9809)
                     /locus_tag="RO454_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDY0935893.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         2446 a         2617 c         2382 g         2456 t
ORIGIN      
        1 tataattccg taacgtttat catgctaccc taaggattat ccgaaaaata atatctgcga
       61 acatcttcca ggatactcct gtagcgaaat atttgtttta agctcactca catatcgcaa
      121 catttacttt actttaagac aattccaggc aaattataca acactttacg ggatagtaag
      181 tccgcctgaa aaattgcgag agtggcgcat taggtgaccc atgttgttcc gtttagtcat
      241 gatgaaatat tcaggtaagg ggaattatcg ttacgcattg agtgagggta tgccatgtca
      301 acgattatta tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt
      361 agaggggtta aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct
      421 tgtgattcac agcgcccttc agtggtgttt attaatgagg actgtttcat ccacgatgct
      481 tctaacagtc agcgcatcaa gcacatcatt aatcaacatc ccaatacgtt atttatcgtt
      541 tttatggcaa ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaatttattg
      601 atcagctcta aatcgattaa accggaatct ctcgacgata tccttggcga tattctgaaa
      661 aaagatacaa cgataacctc gtttttaaat atgccgactt tatcattgag ccgaaccgaa
      721 tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca ttcaaatctc cgaccaaatg
      781 aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaacg
      841 cataataaac aggttatcta ccatgtcgtc cgactgacgg ataatgtgac taatggtatt
      901 tttgtcaaca tgcgctaaca cattctgact ggtggtttcc caccagtcag gctgaataag
      961 attactctgc tttctcaaca aagataccgt cctgatgccc tgcttcatta aagaaccaga
     1021 tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt gccttcacta aactcctcaa
     1081 ctgccagtac cacgccagga cgacgcggac cgccatccgt tttgactgtt acccgatcat
     1141 tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt agaacaattt cgtttttctg
     1201 gcagcgccag gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa gagatgccag
     1261 gcggacttaa cgactggcgg caacaacaga gtaacggttg cgaggaaaga tgatgaaaac
     1321 cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg atgccctgct
     1381 ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc agccagaacg attctgaaca
     1441 cgttagcgtc gatggacgtg aatatcatac atggcgtgaa ttggcggatg ccttcgaact
     1501 ggatattcat gacttcagcg tctctgaagt gaatcgttga atgcacaata aaaaaatccc
     1561 gaccctgagg gggtcgggat gaaacttgct taagcaagaa gcacttaaaa aattcgttac
     1621 accaggaaat ctgatgtgtt catcacctta tctgcaattt ttttcgctga caagaaaata
     1681 ttcgcgctat gaatgattaa cgctacttat gaatagaaat gtgacgcgcg tcaccccttg
     1741 gtggctctgg tgtcaacggg atcgctcccg aaaaaatcat ctttgattat ttgtgctgtt
     1801 ttgagaatca tcctggtaag gggagtactg cgcccatgtt ttcaattcaa caaccactac
     1861 tggtttttag cgatcttgat agcaccctgc tggacagtca tagttatgac tggcaaccgg
     1921 cagccccctg gctcagccgt ttacacgaag ccaatattcc cgtcattctc tgtagcagta
     1981 aaacttcagc ggaaatgctg tacttgcaaa aaatgttggg gctacaaggc ttaccgctga
     2041 ttgcagagaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata gacggttttc
     2101 cacgcatcat ctcaggtatt agccatggcg aaatctgcca ggttttaaat acgctacgcg
     2161 agaaagaaca ttttaaattc acgacttttg atgatgtcga cgatgcaacc attgccgaat
     2221 ggacgggatt aagccgtagc caggcggcgc tgacgcagct gcatgaggcg tcggtaacgc
     2281 taatctggcg cgacagtgac gagcgtatgg cacaatttat cgctcgtctg aacgaactgg
     2341 gcttgcagtt tatgcagggt gcgcgcttct ggcacgtact ggatgcctct gccggaaaag
     2401 atcaggctgc caactggatt accgcgacct atcaacaatt gtcaggcaga cgcccaacca
     2461 cacttggcct gggcgatggg ccaaacgatg cgcctttact ggaggtaatg gattacgcgg
     2521 tgattgtgaa agggctaaac cgtgaagggg tccatctgca tgatgaggat ccggcccgcg
     2581 tctggcgaac gcagcgtgaa ggaccggaag gctggcgtga agggctggac cattttttct
     2641 ccgcccgtta agcgttatcg ctcgcgaata cccgattacg cccggcctgt tttgcgaggt
     2701 aaagccgacg gtcggccaat gactggagtt gttcaaaatc ataatcaccg gtttcctcgc
     2761 tgctacttac ccccagcgag gcactgatgc gtatcgttgt actcttggcg atcagcatct
     2821 ctttttcatt taacttcagg cgaatacgtt ctgcgacctc cgcagcctgc gtcagattcg
     2881 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac
     2941 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
     3001 caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca gaaaaaggat
     3061 gttggtgcgt ctgacacaat ttagcgagcg gacgggcttt ttcgaacaat gcgccacggt
     3121 tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
     3181 acatgttgct gaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga
     3241 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
     3301 ttagcgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
     3361 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc gccagcaagg
     3421 ccagttcacg aggatcaaaa atattccctg ttggatggtc aggatttgaa gaggtcaaaa
     3481 atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttt
     3541 ttaaaaattg ctgcatagta cgcacgggaa tactcatccc cagcacgcca taccagtagt
     3601 tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
     3661 gttgcgaagt gaaccagcgt accgcgcggt gacgattttc tcgttgtgaa tggccgataa
     3721 accagggttg ggtgacatag ccgtaataac gcgttggtac attgcgcgta aacaaggtcg
     3781 gcagcgtcga aacgtaaaat ccagcacgcg agacatacac cgcctgttca accatcgacg
     3841 aggagttgtg cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt
     3901 cgaggctttc attttcgcga gacaggagat tcccctcgct gactaatgca tccgatacgc
     3961 cattgactgg cagggtgcgc cgtcggttga gttcgatttg ccaggcgtgc tcgtcgcgat
     4021 gctgttcgaa ctcggttacg gcattacgca gagaggtgaa atccagtggc gccacgagag
     4081 cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat
     4141 cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag gcatcttcca
     4201 gcaccaccac ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga
     4261 gattaacgac atgaccgggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt
     4321 ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca
     4381 taacccggcg atagcgtacc gcaaaacagc gataatttta taatggtggc atgataagac
     4441 gcgactatca ttcattgcag gcattgggac accaggccgg atgtgatccg gcctggtgtt
     4501 aatgataccc tttcaaactt cttccgcaga acgactcccc tcccccggaa caagctcggt
     4561 ttcatgaaaa gcttcccgct tgaccccgta cccgtcatac caacgacact caaccatacc
     4621 gctggagtat ccagtgacaa tcatccgcgg gccgccctct ttaaccgtaa cttcctcact
     4681 aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc ttagttgaag
     4741 atggcgaatt ttgcataata gcctgtgcga gtattaatgt gcctgaccgc gcattttcgc
     4801 tacggctttc actcccagca ccacgatgcc gccgatgata aatccaagaa tcagatttaa
     4861 aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgaaat gttcaatggc
     4921 gtgatgcaac ggcgcaatac catgtaccac aatcccgccg ccgacgagga acatcgccag
     4981 cgtgccgaca atcgataacg ctttcatcaa ccagggcgca atgatcaata accctttacc
     5041 taatgcctgc atcagcgcgc tggatttttc cgccagccaa taccccaggt catcaatctt
     5101 aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga tgcctgaaag
     5161 caccagcacc tgattaagca acggcgcttc tgccacaatc cccagcgtga tggcgacgat
     5221 ttccgcagac aatataaaat cggtacgaat cgcccctttt attttgtcct tttcaaactt
     5281 cagcggatcc tgcgccgcca gcttctccag acgctgctga ctctgcgccg gatcttcttt
     5341 atgtttacgc gcctccagca tatgcagcac tttctctacc ccttcaaagc agagaaacgc
     5401 gccgccaatc atcaacagtg gcgtaatcgc ccacgggata aacgcactga tgatcagcgc
     5461 cagcggcacc agaatcactt tattgatcag cgaacctttc gccacgcccc agaccacggg
     5521 aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata agtcatcccc
     5581 taatacaccg gcggttttct tcgccgccag tttgcccatc acggagatat cgtccagcag
     5641 tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca ttccctctca
     5701 tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata cttgcttcac
     5761 aggtcaacat ttatttaaca atcaagtgca attatatctc tcgccagcag aatgattttc
     5821 acgtttacta tgaaccacct ttttatttca gccgtgaggg attatgcgtt tccgccagtt
     5881 gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa cctattttgt
     5941 cattcggatt ggcgtggaaa gctggcctcc gttaatgatg gcgggcgttc gattcctggc
     6001 agccggtatt ttattgctgg catttttgct actgcgcgga cacaaacttc ccccgctacg
     6061 tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta atggcatggt
     6121 gacggttgcc gaacatcaaa atgttccttc cggcatcgcc gccgtagtgg ttgcaaccgt
     6181 gccccttttt accctgtgct tcagccgcct gtttggcatt aaaacgcgca aactggaatg
     6241 ggtgggtatc gccattgggc ttgccggaat catcatgctc aatagcggtg gaaacttaag
     6301 cggcaatcca tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc
     6361 ggtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct
     6421 ggcggctggc gtggtgttaa tgattgcgtc gatgattgcg ggtgaaaaac tgacggcgct
     6481 cccttccctt tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc
     6541 cattaacgct tatatgtatt taatccgcaa cgtcagtccg gctctcgcca ccagctacgc
     6601 ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggtggcg aaacgctgtc
     6661 gaagattgaa tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg tcacgttggg
     6721 aaaatatctc ttcccggcca aacccgttgt cgcgccagtt attcaggatg catcaagcga
     6781 gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag atccactctt
     6841 ccagacgctc ggtaagcgcc gcatccgtaa gcttctcgcg cccacgtaac gcacactccc
     6901 agacaatcag tacgcgccag ccgagttcct gcaagcgact gatatcgcgg cgatcgcgtt
     6961 caacattttt acctatcttc tccagccaga actcggttcg agtcgcaggc actttaaaca
     7021 gatagcagtg atgatgatgc cagaagcagc cgtgggtaaa tatcacgcag cgatattcat
     7081 caacgacaaa atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac gcaaggccct
     7141 gcccggttaa caaactggcg aggcgcttct caatcgccgt atcacgcgtg gcaatcgcgc
     7201 gcatattttt gctgcgggtg gccttatcgt gaacgtcgac catgttgtgc ctcttgctga
     7261 cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagacc
     7321 ggcacgacca ccgagttacc gaactggcga taggcctgag tgtccgaaac cggaatgcgg
     7381 aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcacg aggtgttaac
     7441 cgacgtggac gatgttgctg attcagcgga tcgtcaaagt ctttctcacc cgtggccata
     7501 tcccagccgc ggtcgattaa aatttccgcg ccatccttgt aataacgagc agacagcgta
     7561 cgggtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc gttaccgcgc
     7621 gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt caggatatat
     7681 ttcgcctcaa ccatcgggtc cagcagctgc gccagcgtca ctcgctgtgc agggaaacat
     7741 tcgctgatat cacgcagagt aaaatcggct ttcagattca gatcgcgacg aaaacccacc
     7801 agcacgatgc gttcacggtg ctgcggcagg aaatgtttac cgtcgatgat tttcggatcg
     7861 tccggcccgt tatctgctgc gtcggcaacg tcatagccca gttcgtccag cgtctgcatg
     7921 atgatgcgga acgtttttcc cttatcgtga ctcttcaggt ttttgacatt ttcgagcaca
     7981 aacatcgccg gacgacgcgc gtcgataatg cgcaccacat caaagaacag cgtaccctgg
     8041 gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc
     8101 gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat gtgttgacga
     8161 atatgttccg ccgccgcctc atcactcacg ccttcttgat gactgagggt gatgtcgcgg
     8221 atatcttcat taaaatgatg cgtcgccggg tcgcaataat ggttggcttt ataagtgcgt
     8281 acagcatgtt tgttccattc gctggtaaac acgcactgtc cgccaatcga ttcaaaaccg
     8341 cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga
     8401 tgttccggtg gtttgggtaa caacgtttgc agatgggcaa actctttctc acttaaacgg
     8461 tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca
     8521 cccacaccat taagctgcgc caccagcgtt ttaacatcat aaatttgcag cagtttctcc
     8581 agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc
     8641 atagatttaa ccggccatct gagatggccg acaggttaac ataattactg ccgtttaggg
     8701 ccacgcgctc agccagcggg tgaaaacgca tctatcacct tgtgatcgat accttcattc
     8761 tcccccgcca gttcggcacc gagcttcgcc ataaactcga ctaaaaattg cgcgttgtgc
     8821 tgtgccagct gcttgcctct tgcggtttgc atggtttgcg gtaatttcag taactttgtc
     8881 tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc gaacggatct
     8941 tcgccatcaa acagcgccaa gcccaatgcc cctgaaacgg caaatacgcg cgccaggcca
     9001 atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag
     9061 ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcaattttc
     9121 tccgccggaa attgcgcgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg
     9181 attgaagaac gctgccgttg cggatgattt ttcgccaggg tcacgatatc gtgaaaataa
     9241 caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagttt ctgtgccgta
     9301 gcccagacac ggcgaaaatg acacaagtca tgcgccgcgt cctgatgttg atgatggttt
     9361 ttcaaccagt tatcgaactg tgcctgccag tgttgtaact ccatacgcgc tccgttgcca
     9421 aagagtcgca accttagcag tttttattca tagcgtctgc aacgtgcggg acgaggtcgg
     9481 gtgtaccatg caatgccgcc taacaaggca ccaaccaggg ccataaacag aaatccgacc
     9541 aatgctccta accacttccc cgcggtagat cctaaatcac tttcaaatcc cgacacgggg
     9601 gcatcgggga tccggctcat cacccagatg tatcgcgcca ttgcccagac gaaatagcca
     9661 caccatgtat agaaagccca caaagccaat ttgccgccag ggctgcgaga gagtctctct
     9721 tccatctttg tcgaaccgta ttccattttg agataacccg gaaaattcag acttagaact
     9781 attatgtgat aaaagtcaca tttttccaca ttggatgagc cagtcaagac tcaatcattt
     9841 tttgaacaaa tagataaatt ttgcgcaacg gcggtgaata caggaaaaat cgatttgaac
     9901 g
//
LOCUS       DAPQZD010000172         9862 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPQZD010000172 DAPQZD010000000
VERSION     DAPQZD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.174"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(15..176)
                     /locus_tag="RO454_004145"
     CDS             complement(15..176)
                     /locus_tag="RO454_004145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935894.1"
                     /translation="MSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            595..2226
                     /gene="oppA"
                     /locus_tag="RO454_004146"
     CDS             595..2226
                     /gene="oppA"
                     /locus_tag="RO454_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDY0935895.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2312..3232
                     /gene="oppB"
                     /locus_tag="RO454_004147"
     CDS             2312..3232
                     /gene="oppB"
                     /locus_tag="RO454_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDY0935896.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3247..4155
                     /gene="oppC"
                     /locus_tag="RO454_004148"
     CDS             3247..4155
                     /gene="oppC"
                     /locus_tag="RO454_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDY0935897.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4167..5180
                     /gene="oppD"
                     /locus_tag="RO454_004149"
     CDS             4167..5180
                     /gene="oppD"
                     /locus_tag="RO454_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDY0935898.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5177..6181
                     /gene="oppF"
                     /locus_tag="RO454_004150"
     CDS             5177..6181
                     /gene="oppF"
                     /locus_tag="RO454_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDY0935899.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6234..6563)
                     /locus_tag="RO454_004151"
     CDS             complement(6234..6563)
                     /locus_tag="RO454_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDY0935900.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6598..8058)
                     /gene="cls"
                     /locus_tag="RO454_004152"
     CDS             complement(6598..8058)
                     /gene="cls"
                     /locus_tag="RO454_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDY0935901.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8201..8374
                     /locus_tag="RO454_004153"
     CDS             8201..8374
                     /locus_tag="RO454_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDY0935902.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8429..9682)
                     /gene="kch"
                     /locus_tag="RO454_004154"
     CDS             complement(8429..9682)
                     /gene="kch"
                     /locus_tag="RO454_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDY0935903.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2585 a         2295 c         2445 g         2537 t
ORIGIN      
        1 gcttctcctc accatcatgt tattttcgcc acatcataat cctgggcttg ctgaagaata
       61 attgaaatga tattagtaat tccactgcct ttggtagagg aaagtgctaa ataataatca
      121 attgttaaat tattgtgcat ttcactactg gaactgtaat cagaaaagag agacatgctt
      181 aactaacctc tatttgattg aattgaaaga tgtttgttaa ggcatggatg caagctatag
      241 attctggtac ggtcaataaa agagaattgc ttaacaattt tgcaaaatgt attggcgagt
      301 aagaaccgca tttggtactt tccgggcaac cgccaggcga ttctttattg gtaatgagaa
      361 taattaacaa ttaaagaccg tcgcgaaaga ataatgtgtc tcgacagggg agacacagta
      421 cgaatcgaca taaggtgatc gtctgaatca ccagaataaa taaagtcggt gatagtaata
      481 cgtaacgata aagtaacctg acagcagaaa gtctccgagc ctgtgcaggg tcccaatccg
      541 ggattacaca tgctggttaa taccagtaat tataatgagg gagtccaaaa aacaatgacc
      601 aacatcacca agagaagttt agtagcagct ggcgttttgg ctgcgcttat ggcagggaat
      661 gtcgcgctgg cagctgatgt acccgcaggt gtcacactgg cggaaaaaca aacgctggta
      721 cgtaacaatg gttcagaagt tcagtcatta gatccgcaca aaattgaagg tgttccggag
      781 tctaatatca gccgagacct gtttgaaggc ttactggtta gcgatcttga cggccatccg
      841 gcacctggcg tcgctgaatc ctgggataat aaagacgcga aagtctggac cttccatttg
      901 cgtaaagatg cgaaatggtc tgatggcacg ccagtcacag cacaagactt tgtgtatagc
      961 tggcaacgtt ctgttgatcc aaacactgct tctccgtatg ccagttatct gcaatatggg
     1021 catatcgccg gtattgatga aattcttgaa gggaaaaaac cgattaccga tctcggcgtg
     1081 aaagctattg atgaccacac attagaagtc accttaagtg aacctgttcc gtacttctat
     1141 aaattacttg ttcatccatc aacttcaccg gtgccaaaag ccgctatcga gaaattcggc
     1201 gaaaaatgga cccagcctgg taatatcgtc accaacggtg cctatacctt aaaagattgg
     1261 gtcgtaaacg aacgaatcgt tcttgaacgc agcccgacct actggaacaa cgcgaaaacc
     1321 gttattaacc aggtaaccta tttgcctatt gcttctgaag ttaccgatgt caaccgctac
     1381 cgtagtggtg aaatcgacat gacttataac aacatgccga tcgaattgtt ccagaagctg
     1441 aaaaaagaga tcccggacga agttcacgtt gatccatacc tgtgcactta ctattacgaa
     1501 attaacaacc agaaaccgcc atttaatgac gtgcgtgtgc gtaccgcact gaaattgggt
     1561 atggaccgcg atatcattgt taataaagtg aaagcgcagg gcgacatgcc cgcctatggt
     1621 tacactccac cgtatactga tggcgcaaaa ttgactcagc cggaatggtt tggctggagc
     1681 caggaaaaac gtaacgaaga agcgaaaaaa ctgctggctg aagcgggtta taccgcagac
     1741 aaaccgttga ccatcaacct gttgtataac acctccgatc tgcataaaaa gctggcgatt
     1801 gctgcctctt cattgtggaa gaaaaacatt ggtgtaaacg tcaaactggt taaccaggag
     1861 tggaaaacgt tcctcgacac ccgtcaccag ggcacttttg atgtggcccg tgcaggctgg
     1921 tgtgcagact acaacgaacc aacttccttc ctgaacacca tgctgtcgaa cagctcaatg
     1981 aataccgcgc attataagag cccggccttt gacagcatta tggcggaaac gctgaaagtg
     2041 actgacgagg cgcagcgtgc agctctgtac actaaagcag aacaacagct ggataaggat
     2101 tcggccattg ttcctgttta ttactacgtg aatgcgcgtt tggtgaaacc gtgggttggt
     2161 ggctataccg gcaaagatcc gctggataat acctacaccc ggaatatgta cattgtgaag
     2221 cactaatggc aatacgtggg gcaggagtgt cctgctccac ggtgtctgat ttttatcgca
     2281 ttacagaagg cacaggccag aaggtagggc aatgttaaaa tttattctac gtcgctgtct
     2341 ggaagcgatt ccgacgctat ttattcttat tactatttcg ttctttatga tgcgcctcgc
     2401 gccgggcagt ccttttaccg gcgaacgtac tttaccaccg gaagtgatgg ccaatatcga
     2461 agcgaaatat catcttaatg atccgatcat gacacagtat ttcagctacc tgaaacaact
     2521 ggcgcatggc gatttcggtc catcgtttaa atataaagat tattcggtca atgacctggt
     2581 tgcatccagt tttcccgttt ctgccaaact gggagctgca gcattttttc ttgcggtaat
     2641 actgggtgtt agtgctggcg ttattgccgc attaaagcaa aacaccaaat gggactatac
     2701 cgtgatgggg ctggcaatga ccggggttgt tatccccagt tttgtggttg cgccattatt
     2761 agtcatgata tttgcgatca ttttgcattg gctgccgggc ggtggctgga atggtggagc
     2821 gcttaaattc atgatattgc caatggtggc gttatcactc gcctatatcg ccagtattgc
     2881 gcgtattacc cgtggctcta tgattgaggt tttacactcc aactttattc gtactgcccg
     2941 ggcgaaaggg ttacctatgc ggcggatcat tttacgccac gcattaaaac ctgctctgtt
     3001 acccgtgctc tcctatatgg ggcccgcatt tgtcggcatt attaccggtt cgatggtcat
     3061 cgaaaccatt tatggtttac cggggattgg gcaattgttc gttaatggcg cattgaaccg
     3121 tgactattcc ttagtgttaa gtctgaccat tctggttggc gcgttaacca ttttgtttaa
     3181 tgccattgtc gatgtgctat atgcggttat cgacccgaaa atccgttact gatactggag
     3241 ctcgcgatga tgttaagtaa gaaaaacagc gagacgctgg aaaatttcag tgaaaagctg
     3301 gaggtcgaag ggcgcagctt gtggcaggac gcacgtcgac gttttatgca taaccgtgcg
     3361 gcggttgcca gtctgatagt gctggtgctg atcgcgttat ttgtaatcct ggcaccgatg
     3421 ctttcgcagt ttgcctatga cgatactgac tgggcgatga tgtccagcgc cccggatatg
     3481 gagtccggtc actactttgg tactgactca tccggtcgcg acctgcttgt gcgcgttgcg
     3541 attggcgggc gtatctcact catggtcggt gttgctgcgg cactggtggc agtggtcgtg
     3601 gggacacttt atggttcgct ttccggttat ctgggcggta aagtggattc ggtaatgatg
     3661 cgtctgctgg aaatcctcaa ctccttccca ttcatgttct tcgtcatttt gctggtgacc
     3721 tttttcggtc aaaacatcct gctgattttc gtggcgattg gcatggtttc ctggctggat
     3781 atggctcgta ttgtgcgtgg gcaaaccctg agtctgaagc gcaaagagtt tattgaggcg
     3841 gcacaagttg gcggtgtatc gacgccgggc attgttattc gccacattgt gccgaacgta
     3901 ctcggtgtgg tggtggtcta tgcgtcgctt ctggtgccca gcatgatcct ctttgaatct
     3961 ttccttagct tcctgggatt aggtacacaa gagccgttaa gcagctgggg tgcattgctg
     4021 agtgatggcg cgaactcgat ggaagtctct ccatggttac tgttgttccc agcgggattc
     4081 ctcgtggtaa cgctgttttg tttcaacttt atcggcgatg gcttgcgtga tgccctcgac
     4141 ccgaaagatc gttaaggagt gcagccatga gcgtaattga aactgcaact gtgccgctcg
     4201 cacaacaaca ggctgacgca ctgctgaacg tgaaagattt gcgtgtcacc tttagtaccc
     4261 cggatggcga cgtcacggcg gtcaatgatt tgaatttttc gctacgtgcc ggagaaacgc
     4321 tgggcatcgt aggtgagtct ggttcgggca aatcgcaaac cgcattcgcg ttgatgggcc
     4381 tgttagctgc caacggacgt attggcggat cggcaacctt caatgggcgt gaaatcctca
     4441 atttgccaga gcatgaactc aataaactgc gagctgaaca aatctcaatg atttttcagg
     4501 acccaatgac ttcgttgaat ccctatatgc gcgtcggtga gcagttgatg gaagtgctga
     4561 tgctgcataa gaacatgagc aaagctgagg cgtttgaaga atcggtgcgg atgctcgatg
     4621 cggtaaaaat gccggaagcg cgtaaacgca tgaaaatgta cccgcacgaa ttttctggcg
     4681 gcatgcgtca gcgagtcatg attgcgatgg cattgctatg tcgacctaag ctgctgattg
     4741 cggatgaacc cactactgcg ctggacgtca ccgtacaggc gcagatcatg acgctattga
     4801 atgaactgaa gcgggaattt aataccgcca tcattatgat tacccacgat cttggcgtgg
     4861 tggcggggat ctgcgacaaa gtgctggtaa tgtacgccgg gcgcacgatg gaatatggca
     4921 acgcgcgcga tgtcttttat caacccgtac atccttattc tatcggtttg ctcaacgcgg
     4981 tgccgcgtct cgatgcggaa ggtgaaacaa tgttgactat tccgggtaat ccgccaaacc
     5041 tgctgcgatt accgaaaggt tgcccgttcc agccacgttg tccgcatgcg atggatattt
     5101 gtagtagcgc accgccgctg gaagagttta cgcctggccg tctgcgtgct tgctttaaac
     5161 cggtggagga actgttatga atgctgtaac cgaaggaaga aaagtcctcc tcgaaatcgc
     5221 cgatctgaaa gtgcactttg aaatcaaaga tggcaaacag tggttctggc aaccgccgaa
     5281 aacgctcaaa gccgtcgatg gtgtaactct tcgcctgtat gaaggggaaa cattaggtgt
     5341 ggtaggggaa tcgggatgcg gtaagtccac ctttgctcgc gccatcattg gcctggttaa
     5401 agcgaccgac ggtcatgttg cctggttagg taaagagttg ctgggcatga agcccgatga
     5461 atggcgtgcc gtacgcagtg atattcagat gattttccag gatccgttgg catcgctgaa
     5521 cccgcgtatg accatcggcg agatcatcgc tgaaccactg cgtacttatc atccgaaaat
     5581 gtcacgccag gaagttcgcg agcgcgtgaa ggcgatgatg ctgaaagtcg gtttgttgcc
     5641 taacctgatt aaccgctatc cgcatgagtt ctccggtggg cagtgccagc gtatcgggat
     5701 tgctcgcgct cttattcttg aaccgaagct gattatctgt gatgagccgg tgtcggcgct
     5761 ggacgtgtca attcaggcgc aggtggtcaa cctgctccag cagttgcaac gtgagatggg
     5821 attgtcatta atttttatcg ctcacgacct ggccgtggta aaacacattt ccgatcgtgt
     5881 gctggtgatg tatctcggcc atgcggtaga actggggacc tatgatgagg tctaccacaa
     5941 tccgctacat ccttacacca gggcattgat gtcggcagtc cccatacctg atccggatct
     6001 ggagaagaac aaaaccatcc agttactgga aggggaatta ccgtcgccga tcaacccgcc
     6061 ttccggttgt gttttccgta cccgttgtcc gattgcaggt ccggagtgcg ccaaaacacg
     6121 tcctgtgctg gaagggagtt tcagacacgc cgtttcttgc ctgaaagtcg atccgcttta
     6181 aaagcaataa gggctgacag ttgtcagccc ttattcacgc caaaagcgat tatttattcc
     6241 cgccagatga tatggcaaag tttgtgatct ttttcgcggc ataacagaat gcgcgcgaaa
     6301 acatcgttga tttcaccatc ttcactgtcc gccagaccaa tcaccacttc ggcaaaaaag
     6361 tccgggttca aatcgaaatc aacatgttcc tgccagtctt ccgccggatc gaataactcc
     6421 gcgccgccgc gctcttcaaa ctgaagattg aacagcagaa catcagctgg atcgagattg
     6481 tcggcagcca gttcgagaaa aatatcgtag gcctgctcaa gcgtttcatc ttcagtcagg
     6541 cgattgttta gatccatatc catagtcact acctgtttaa cctctgttgg cgacgtttta
     6601 cagcaacggg ctgaagaagt aaaacagtcg ctcggcgaca cgttgccata atggacgttt
     6661 tagccataaa cgggcatcga gcagacgaga acgcgaaata taatcgtcct gaacggcggc
     6721 gaggtctgca ccaaaacctt tatcgtcgat tgccagggta atctcgaaat ttagccacag
     6781 actacgcata tcaaggttaa cggtgccaac cagacttagt tctccatcga ccagcacgct
     6841 cttggtatgc agtaacccgc cttcaaactg ataaatttta accccagcag ccagcagttc
     6901 tgtaaagaat gcgcgactgg cccagccgac cagcatcgag tcattttttc gcggaaggat
     6961 aatactgaca tccaccccgc gctgcgccgc cgtgcaaatc gcatgaagta aatcatcgct
     7021 tggcacaaaa tagggcgttg tcatgatcaa atattcacga gccgaataag cagcagtcaa
     7081 taatgcctgg tgaatgagat cttccggaaa gccggggcca gaagcaattg tgtgaatggt
     7141 gtgaccactg gcctgctcaa acggcataat attgacatct ggtggcggcg gcagaatacg
     7201 tttcccggtt tcaatctccc agtcgcagga ataaataatc cccatcgcgg tggcgatggg
     7261 gccttccata cgcgccatca gatcaatcca ttgccctaca cccgcatctt gtttgaagta
     7321 gcgagggtcg accatattca tgctgccggt gtacgcgatg taattatcga tcatgatcat
     7381 cttgcgatgt tggcgcaggt ccatacggcg taaaaacaca cgcatcagat tgacctttaa
     7441 ggcttcgaca acttcaatac cggcattacg cattagctcg ggccacgggc tgcggaaaaa
     7501 agccacactc ccggcggagt cgagcatcaa tcggcaatga ataccgcgcc gtgcagcggc
     7561 cattaatgat tcagccacct ggtccgccat gccgccgggc tgccagatat aaaacaccat
     7621 ctcaatatta tggcgcgcga gctggatgtc gcggattaac gcctgcatca catcatctga
     7681 ctcggtcatc agttgtagct gattcccttt gaccccagcg atcccctgac gacgctcgca
     7741 aagcttgaat aatggcgcag cgacactgct attttcttcg gcgaagatat gcttacaggc
     7801 tttaaggtcg ttaagccatt ttgcggtgga aggccacatc gctctggcgc gctcagcgcg
     7861 gcgtttgcct aaatggagct cgccaacggc aagataggca ataattccga ctaacggcag
     7921 aatgtaaata atcaacagcc aggccatcgc ggagggaact gcgcgtcgtt tcattagaat
     7981 gcgtaaagtt acgcctgcaa tgagcaacca gtatcccaga atggccaacc aactcaccaa
     8041 cgtataaacg gttgtcatag attagaaatc cttttgaaag cgcattgtta tgagtttacg
     8101 catatggatt catctggcaa ataaaaacga gggaaaagca ctggtcagcg tcaggggcgg
     8161 gggtataatg accattctgt tgttgcatag agtagttaac atgaagcgga gtagaacgga
     8221 agtggggcgc tggcgcatgc agcgtcaggc tagccgacgt aaatcgcgtt ggcttgaggg
     8281 gcagtcgcgc cgaaatatgc gtatccacag catcaggaag tgcattctaa acaaacagcg
     8341 taactcgtta ttgtttgcga tctacaatat ctaaatgtac tgggcaccat tgcggtgccc
     8401 aataagttta ctcttccgtc tttaaactct atttttgcgc cgattcttta ctgtcagctt
     8461 tcgtttcttg ttcatcgtta tcgctgaaaa tgccatgacc ggagttcaac aacattgaaa
     8521 cgagcatatc attattaatc tcttcaccat ttaaaactcg cgccagaatt tcgctgccaa
     8581 acagttgtgg tgagagaatg atatccggat gtaccatctt aatcttattc aggtttttgc
     8641 tatcactgac ggcaagaact gttttgacat cactgctcat atcttttgcc gagagtacaa
     8701 caaacgcgtt atctgcatcg ttatcactca gcgccagaat ggcccggcaa cgatcgattc
     8761 ccgctttctt taacactgaa ctgtcattac tgtcaccggg gataacatcg gcgttatcgc
     8821 ctaagcgttg ctcaagttgc ttgatatcat cttcaggcaa gttgctgata accgttacgt
     8881 tttgtccgcg ttgattcagt tgcagaatcg tattgatggc gagaatcgaa tgtccgcaaa
     8941 caataaaatg atctttacga tgcattgtat gattgtttcc ttttacaagt ttgttgaatc
     9001 ccccgcggat aagcggacca aaaattgagg tcatagatgt ggcaaaaacg gtaatgccgg
     9061 aaataatgac cgaaatagtg aacaatcgtg ctgattcaga aacagggaca atatcgccat
     9121 agccgacggt tgacatggtt tctatcgaaa aatagaacgc ggtcatcaaa ctttctattc
     9181 gcggattaaa accttcgctt aaataaagcg caccgtaagt ggagtaaaac agtaacgtcg
     9241 tgaaactaat aaatgcgaaa attgtcccgg ctgctgcgct actgtgggag aagtctttgc
     9301 tcagtatcag caaaaacacc agcgtaaaaa tgcaaaatcc aatactaaat ttcagccagg
     9361 gataaaaatg cagagtgtaa attagcgcta tcaacaacaa aatgatactg attgcccatg
     9421 cgagctttgc ccggaacagt aagccaatgg agttcaggac aagaaatacg ccgagcataa
     9481 atagtggggc gtttgccagc aaggagagat ccagctctga aaatgctttg atatggaaaa
     9541 tatcaagcaa attgaccgac ataccataga ttgttttaaa aataagcaat ccatttaaaa
     9601 agacggccag cgcgagaatg tcgtgccgta gggtcaccca taactttgtt gctgtttgtt
     9661 tgaatgtagt ccagtgactc actgatacct ctgtaaccac aattttgttt aattatctca
     9721 acgtgcacat tttctgaaca tacatgcagc tcgtgaatgt gttaaacaat agtagatgac
     9781 tttatccgca gtaatcaaag gttttgagtg agcaactctg cgtcaggaaa gggcattttc
     9841 ctgaatatgt tcacatcata ta
//
LOCUS       DAPQZD010000173         9850 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPQZD010000173 DAPQZD010000000
VERSION     DAPQZD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.175"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..406)
                     /gene="folK"
                     /locus_tag="RO454_004155"
     CDS             complement(<1..406)
                     /gene="folK"
                     /locus_tag="RO454_004155"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDY0935904.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPE"
     gene            complement(403..1821)
                     /gene="pcnB"
                     /locus_tag="RO454_004156"
     CDS             complement(403..1821)
                     /gene="pcnB"
                     /locus_tag="RO454_004156"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDY0935905.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1860..2786)
                     /gene="gluQRS"
                     /locus_tag="RO454_004157"
     CDS             complement(1860..2786)
                     /gene="gluQRS"
                     /locus_tag="RO454_004157"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDY0935906.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQNIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2823..3278)
                     /gene="dksA"
                     /locus_tag="RO454_004158"
     CDS             complement(2823..3278)
                     /gene="dksA"
                     /locus_tag="RO454_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDY0935907.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3456..4160)
                     /gene="sfsA"
                     /locus_tag="RO454_004159"
     CDS             complement(3456..4160)
                     /gene="sfsA"
                     /locus_tag="RO454_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDY0935908.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4175..4705)
                     /gene="thpR"
                     /locus_tag="RO454_004160"
     CDS             complement(4175..4705)
                     /gene="thpR"
                     /locus_tag="RO454_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDY0935909.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4779..7208
                     /gene="hrpB"
                     /locus_tag="RO454_004161"
     CDS             4779..7208
                     /gene="hrpB"
                     /locus_tag="RO454_004161"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDY0935910.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFLRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPRLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7302..9827
                     /gene="mrcB"
                     /locus_tag="RO454_004162"
     CDS             7302..9827
                     /gene="mrcB"
                     /locus_tag="RO454_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDY0935911.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
BASE COUNT         2256 a         2514 c         2816 g         2264 t
ORIGIN      
        1 gctccggcgc aatttcaaac agtggccaca gcataaagcc acgatttttc atatcgtagt
       61 gcggaacggt cagacgttcg gtatttatta cttcattacc aaacagcatg atgtcgagat
      121 ccagcgtgcg cggtccccag cgttcagctt tacggacgcg accttgctgc aattcgatac
      181 gctgtgtgtg attgagtagc tcttcagggg caagagtggt tttcagcgcg acggcggcat
      241 ttaagtaatc gggttgatct tgcggcccca gaggtggggt gcggtaaaac gaagaaacgg
      301 caagaatgcg gctttctgga atatcgccta atgctttcag ggcagcattg acctgctcca
      361 gcggagaggc cagattgctg cctatggcaa tatacgccac tgtcatgtag taccctcacg
      421 acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc ggtgacggtt cttcatccag
      481 ttcgttaagc atcccttttt ggtctggcgg cgcggaaacc tggaactcac cccaccattt
      541 caccagacgt tgcagttcag cgttacgctc aacttcggct cgtaaggcca acaggtcgta
      601 tgccgcacgg aacttaggat gctccagcag tttccatgcg cgtttaccct gacgacggga
      661 catacgcaac tgcaactgcc agatatcgcg agttagcgta gtcagacgtt tcgggatcgc
      721 cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc agtgcgaaag cgtcgtgata
      781 ggtcaggccg ctctcctgag caattttctg tgccgtctcc agcaatgggt accagaacat
      841 ggcggcaaac aggaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt
      901 cttcagtacc tgctcaataa tgcgttccat cgggctgtcg ccattttccg tgaagtagcg
      961 ggtaatggtc gggaacagcg gctggaacag atgatattca cacagcagct tataggtttc
     1021 gtaaccgtag cccgcttgca acagtttcag cgattcttca aacagacgtg ccggtgggat
     1081 atcgttcagt aaggttgcca ggcgtgggat cggctcggcg gtttccgggc tgatgcgcat
     1141 gcccaatttg gcggcaaaac gaaccgcgcg gagcatacgt accggatctt cacggtagcg
     1201 cgtttccggg ttaccaatca gacggataac gccgtctttc agatccttca tgccgccaac
     1261 gtaatcacgg acggtaaaat ctgccacgct gtaatacagg ctgttgatag tgaaatcgcg
     1321 gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca taccgttttg
     1381 cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg tgatgtccac ggaaggtcgc
     1441 aacttcgata atctccgggc caaacatcac atgagccaga cggaaacggc gacccaccag
     1501 acggcagtta cggaacagtt tacgtacctg ttcaggcgtg gcgttagtgg ttacgtcaaa
     1561 atctttcggc tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc
     1621 gtatcccgct ttattgagcc tgtacattac cttcagggca ttttcactga tatctttgcg
     1681 ggaaatagca tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc
     1741 agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat
     1801 agtacacctc ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct
     1861 cagcatgacg catttgagaa tgttgaattt acaattgccg actcaggcac tgcggtgagc
     1921 cgccagtttt tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgtt
     1981 tctacctgct gacccagaaa atgaagtgcc gcgattaaca ccgggcgcgg atcgcctttc
     2041 ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc
     2101 ggtaaatgga tgtaatctgg cactttccag ccaaaaagct ggtacagcga gatttgccgc
     2161 acagtcggtt cgatcaaatc tgccccgcgg acaatttcgc tcacgccctg gaagtgatca
     2221 tccaccacca ccgccaggtt ataggcaaac aaaccatcac ggcgatgaat aataaaatct
     2281 tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca gcaggtcagt aaactgggtg
     2341 actggatgct gctggcggat acgtactgcg gcgttgtctg gtccatgatg caaatcccgg
     2401 caatgaccgt cgtaaatacc gccaatgttt tgaatacgcg cacgcgtgca ggtgcagtaa
     2461 taacttagtc cttgctcatg taaccaggcg agtgcttcgc gataggcgtg gtgacgttgc
     2521 gattgccaga gaacgtcgcc gtcccagtgt aggccgtaat gttccagctg gcgcaggata
     2581 gtctctgcgg caccgggaac ttcacgaggc gggtcgatat cttcaatgcg taccagccag
     2641 cgaccttgcc gggcgcgagc ctgcaaatag ctgccaagcg cagcaatcag agaaccaaaa
     2701 tgaagctcgc cggaaggaga gggggcgaag cgaccaatat agtgtgtgtc tgtcatctct
     2761 ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt aaacatgatg gaacggctgt
     2821 aattagccag ccatctgttt ttcgcgaatt tcagccagcg tcttgcagtc gatgcataga
     2881 tcggctgtcg ggcgcgcttc cagacggcga ataccaattt caacaccgca ggattcgcag
     2941 tagccgaaat cttcgtcttc cacttttttc agcgtcttct cgatcttttt gatcagctta
     3001 cgctcgcgat cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct
     3061 accgggtccg ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca
     3121 tccctgagtt gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca
     3181 ttcatatact cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata
     3241 ctcagggacg atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac
     3301 tatcgatccc catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg
     3361 atagcaatta tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg
     3421 cgaccagaat gtaaattaac cagttactta ctctactaca atgtaaccgg tagtgatttt
     3481 ttaagagcca tgccttcggc agaaagttcc gctttgtaag ccagaatttc tacccccctc
     3541 tgttgagctt ctgacaatag ttgtgcgtat ttctcatcga tgtggcgcgc gggtgaaaac
     3601 cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg cacgctggcc ttcagccgct
     3661 acgctcatca actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat
     3721 ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa
     3781 tccgcctgca acataaagtc aatacggctg cgttcggcgc cgtattttac ttcgcttttc
     3841 agcgagctat agcctgacag ctctgaaatt gattcattaa ggatagcctc tttcgtcaac
     3901 ctgttagccc aaagcgtatt gacgcaaata attgcgccgc tttggctttg agttaattcc
     3961 caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca
     4021 ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca
     4081 tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt
     4141 agagggggag aaaattccat tgcgacaatc cttgttattg cgttagcgcc cagcgtttta
     4201 gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca
     4261 ccgtatacga ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca
     4321 ataaggtaat atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct
     4381 gtgaacggag catattcgcc agctggatta agccgcgtgg cggctgtcgc atccctaacc
     4441 acaccacacg cgaacgcagc cattgaccgg cgtcatcaag cgtgagtgtg aaaccaggtt
     4501 gacgaatccg tccggctaaa agagaaagcg ccttctcttt ctctgcgctc acttcgccta
     4561 aaaatgccag agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga
     4621 agtgtgtggc gcgccagtgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa
     4681 agaacagccg ttgcggttca gacatgtgag gcactcggtt atgaattacc gcgatgctac
     4741 aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg
     4801 ccgtcttacc tgagttactt gctgccctcg atggtgcatc gcaggtgtta ttaagtgcgc
     4861 cgaccggggc cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta
     4921 acgggaaaat tatcctgctg gagccgcgtc gtctggcggc gcgtaacgtt gcgcaacgac
     4981 tggcggagct gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa
     5041 actgcgtcgg gccgaatacc cgcctggaag tggttaccga aggcgtgctg acgcggatga
     5101 tccagcgtga cccggaactg agcggtgttg gattggtgat ccttgatgaa tttcatgagc
     5161 gcagcttgca ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg
     5221 atgaccttaa actgctgatt atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc
     5281 tgccagaagc gcccgtcgtc atctcagaag ggcgctcgtt tccagttgaa cgccgttatt
     5341 tgccgctgcc cgctcatcag cgttttgacg aagccgtcgc ggttgccacc gccgaaatgc
     5401 tgcgtcagga aagtggatca ttactgttat ttttacctgg cgtcggagaa attcagcgtg
     5461 tgcaggaaca actgacttca cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg
     5521 cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc accgcaaggg atgcgtaaag
     5581 tggtgctggc gaccaatatt gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg
     5641 tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg tacagggctt acgcggctgg
     5701 ttactcaacg tgttagtcag gcgtcgatga cgcaacgtgc cgggcgtgcc gggcgtctgg
     5761 agccgggtat ctgcctgcat ttaatcgcca aagaacaagc agaacgcgct gccgcgcaaa
     5821 gtgaaccgga aatattacaa agcgatcttt cctgtttgct gatggaatta ctgcaatggg
     5881 gatgcagcga tccggcgcag atgagctggc tggatcaacc gccaacggtg aatctactgg
     5941 ccgcgaaacg cctgttacgg atgctggggg cgctggacgg tgaacggctt agtgcgcaag
     6001 ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga
     6061 agaacgacga cgaagctgct accgcggcaa aaattgccgc cattctcgaa gagccgccac
     6121 ggatgggcaa tagtgacctg ggcgtggcgt ttttgcgcaa tcaacccgcc tggcagcaac
     6181 gtagtcagca actgttaaaa cgtttaaacg tacgcggcgg tgaggcagac agttcgctta
     6241 tcgcgccgcg acttgccgga gcgtatgccg atcgtattgc tcgccgccgt gggcaagagg
     6301 gacgctatca actggcgaac ggcatggggg cgatgctcga tgccgacgat gcgcttagcc
     6361 gccacgaatg gttgatcgcg ccgttattat tgcagggcag ttcttcgccg gatgcgcgga
     6421 ttttactggc gctgccggtc gatattgatg agttagtaca acgctgcccg cagctggtac
     6481 agcagtctga caccgtggag tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc
     6541 tgcaaatcgg tcagttgacg gtaaaagtgc aaccgctggc gaaaccctcg gaagacgagt
     6601 tgcatcaggc gatgcttaac ggcattcgtg ataaaggttt aagcgtgctc aactggacgg
     6661 cggaagcgga acagctacgc ttgcgtttgt tatgtgccgc aaagtggttg ccggaatatg
     6721 actggccagc ggttgatgat gaaagtttgt tggcgacgct ggaaacgtgg ctactgccgc
     6781 atatgacagg cgtacattcg ctacgcggcc tgaaatcact cgatatttat caggcactgc
     6841 gtggattact tgattgggta atgcagcaac gtctggatag tgaattgcct gcgcattaca
     6901 ctgtgccgac gggaagccgg atcgccattc gttaccatga agataacccg cccgcgctgg
     6961 cggtgagaat gcaggagatg tttggtgagg cctctaatcc gactatcgcc caggggcgcg
     7021 taccgttggt gctggagttg ctttcccctg cccaaaggcc gctgcaaatc acgcgtgatt
     7081 tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc
     7141 ccaaacatgt ctggccggac gacccggcaa acaccgcacc gacgcgacga acgaaaaagt
     7201 attcgtgaag accaaagcaa atttgagaga tatcttcttc tgtcttgcaa cagaagaaca
     7261 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
     7321 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
     7381 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
     7441 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
     7501 ggctgctgct aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
     7561 tcgatcaaaa aattcgcagc cgtattgatg gcaaggtctg gcaattgccc gcggcagttt
     7621 atggtcgaat ggtcaacctt gagccagaca tgaccatcag caagaacgag atggtgaagc
     7681 tgctggaggc gacccagtat cgccaggtgt cgaaaatgac ccgtcctggt gagtttaccg
     7741 tacaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agcaaagaag
     7801 ggcaggtgcg cgcgcgtctg acctttgatg gcgatcatct ggcgacgata gtcaatatgg
     7861 agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg cctgatcacc atgatctctt
     7921 cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg tttcccggat ttgctggtgg
     7981 atactttgct ggcgacagaa gaccgtcatt tctacgagca tgatgggatc agtctctact
     8041 caatcggacg tgcggtgctg gcaaacctga ctgccggacg cacggtgcag ggggcgagta
     8101 cgctgacgca acagttggtt aaaaacctgt ttctctccag cgagcgttct tactggcgta
     8161 aagcgaacga agcctacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     8221 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     8281 tcccgctggc gagcttgtat tactttggtc gcccggtaga agaattgagc ctcgatcagc
     8341 aggcgctgtt ggtgggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga
     8401 aactggcgct ggagcgacgt aacctggtac tacgtttgct gcaacagcaa caaattattg
     8461 atcaagaact ctatgacatg ttgagtgcac gtccgctggg tgttcagccg cgcggtgggg
     8521 tgatctctcc tcagccagcc tttatgcagc tggtgcgtca ggagctgcag gcgaaattag
     8581 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     8641 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     8701 tgagcgatct tgaaaccgcg attgtggttg ttgaccgctt tagcggtgaa gttcgtgcga
     8761 tggtcggtgg ctctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     8821 cgattggttc ccttgcaaaa ccagcgactt atctaacggc cttaagccag ccgaaaatct
     8881 atcgtttgaa tacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggccagg
     8941 tctggtcacc gcagaacgat gatcgtcgtt atagcgaaag tggcagggtg atgctggtag
     9001 atgcgttgac ccgttcgatg aacgtgccaa cggtgaatct gggcatggcg ttggggctgc
     9061 ctgcggttac ggatacctgg atcaaactgg gtgtgccgaa agatcaactg aatccagttc
     9121 cagcaatgtt gttgggggcg ttgaacttaa cgccaatcga agtggcgcag gccttccaga
     9181 ctatcgccag cggtggtaat cgtgctccgc tttccgcgct acgttcagtc atcgcagaag
     9241 acggcaaggt gttgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg
     9301 cgtatctgac actatggacc atgcagcagg ttgtacaacg cggtacggga cgtcagcttg
     9361 gggcaaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     9421 atacctggtt tgcgggcatt gatgggagca cggtgaccat cacctgggtc ggccgtgata
     9481 acaaccagcc gaccaaactg tatggtgcca gcggggcaat gtcgatttat cagcgttatc
     9541 tggctaacca gacgccaaca ccgctgaatc ttgttccgcc ggaagatatt gcagatatgg
     9601 gcgtggacta cgacggcaac tttgtttgca gcggtggtat gcgtgtcttg ccagtctgga
     9661 ccagcgatcc gcaatcgctg tgccagcaga gtgagatgca gcagcaaccg tcaggcaatc
     9721 cgtttgatca gtcttctcag ccgcagcaac agcaacctgc tcaacaggag caaaaagaca
     9781 gcgacggcgt ggctggctgg atcaaggaga tgtttggtag taattaatat cgtaatcgct
     9841 ctccgcttat
//
LOCUS       DAPQZD010000174         9843 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPQZD010000174 DAPQZD010000000
VERSION     DAPQZD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.176"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            200..1294
                     /gene="ddlA"
                     /locus_tag="RO454_004163"
     CDS             200..1294
                     /gene="ddlA"
                     /locus_tag="RO454_004163"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDY0935912.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1318..1530)
                     /locus_tag="RO454_004164"
     CDS             complement(1318..1530)
                     /locus_tag="RO454_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDY0935913.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1790..2098
                     /locus_tag="RO454_004165"
     CDS             1790..2098
                     /locus_tag="RO454_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDY0935914.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2157..3251)
                     /gene="yaiW"
                     /locus_tag="RO454_004166"
     CDS             complement(2157..3251)
                     /gene="yaiW"
                     /locus_tag="RO454_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDY0935915.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3264..4484)
                     /gene="sbmA"
                     /locus_tag="RO454_004167"
     CDS             complement(3264..4484)
                     /gene="sbmA"
                     /locus_tag="RO454_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDY0935916.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRVTPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4836..5993
                     /gene="ampH"
                     /locus_tag="RO454_004168"
     CDS             4836..5993
                     /gene="ampH"
                     /locus_tag="RO454_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDY0935917.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5994..6617)
                     /gene="iprA"
                     /locus_tag="RO454_004169"
     CDS             complement(5994..6617)
                     /gene="iprA"
                     /locus_tag="RO454_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDY0935918.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6703..9690)
                     /locus_tag="RO454_004170"
     CDS             complement(6703..9690)
                     /locus_tag="RO454_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0935919.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2549 a         2443 c         2305 g         2546 t
ORIGIN      
        1 catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt gcacacatct
       61 ttgagataac gcactttctt caccagtacg ttgctcgctt tgtcagcaga agattgcaac
      121 attctgtcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta
      181 gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg gtggtaaatc
      241 agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag
      301 tcgcttcgat gttgtgctgc tgggcattga taaacaaggg caatggcacg tcagcgatgc
      361 cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgtc cttcggcgac
      421 cagccttgcg caggtgccag gtaaacatga gcatcagctt atcgacgcac aaaacggtca
      481 gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg gcgaagatgg
      541 ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg atgttctggc
      601 ttcagcggcc tgtatggata aagatgtcac caaacgtctg ctgcgcgatg ccgggctgaa
      661 cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt ttgccgaagt
      721 ggagtctaaa ctggggttac cgctgtttgt aaaaccggct aatcagggct cttctgttgg
      781 tgtcagcaaa gtaaccagtg aagaacagta cacaattgcc gtcgatctgg cgttcgagtt
      841 cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gaaatcgaat gcgcagttct
      901 tggcaacaat aacccgcaag caagcacctg tggcgagatc gtactcacca gcgatttcta
      961 tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat
     1021 tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc agacgttggg
     1081 atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag tggtgatcaa
     1141 cgagatcaac accctgcctg gcttcaccaa catcagtatg tatccgaagc tgtggcaagc
     1201 cagcggtctg ggttacaccg acctgatcac gcgtttgatt gagctggcgc tggagcgtca
     1261 cgctgcggat aacgcactga aaaccacaat gtaatattca aacgcccggt aatattctta
     1321 ttgggcgttt tcagtttctt catcgcgacg acgaataatc aatcccgcca gccagaaact
     1381 aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt caaatcccag
     1441 taaccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat agtagtgcca
     1501 gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa acaacatctt
     1561 ttcatattct gtaacaactg tctgtggtgt agacaacact ttcgcaattg ttaataaatt
     1621 taacttaagg ttgctatttt gtgactcgca acgaagattc acgcttcggg aatattccca
     1681 acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga cttgccataa
     1741 gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta tggctgattt
     1801 caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa
     1861 tattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcacaat tgctgaacct
     1921 gctagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa caggtcgccc
     1981 acgggtggaa attggtttag gtgtcggcac catttttggg ctgatcccgt ttttagtagg
     2041 ctgcctcatt tttgcagtag tggcgctatg gctgcactgg cgacatcgcc gccagtaaga
     2101 tttacgttgc cagatgcggc ctaaaacgcc ttatctggcg tgacattcct gtttgatcac
     2161 tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc cgtagtcaga
     2221 ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg gggtagtgat
     2281 ttaccggttt tcgcttcggc aagttgataa actttcttgt acagcgccgt ctcctcaaaa
     2341 gagaagctgt cccctttctc taactgacgg cgaatttcgc tgtcactcat ccccagtttt
     2401 cccgccagtt tgcgcgttgc cagttcggtt ttcccgggct ctttactgtc gtaacgaatt
     2461 aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgaccgcgtt ctgaaacgcg
     2521 gcattacggc tggcgtacca gccagcgtta aaatcagcaa aacggtatat cggcgcgcta
     2581 tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc gcggcgactg
     2641 aagacttcct gacggactgt accgtccatt ttccacggat accctttggt atgctgttcg
     2701 gcaaaagcaa tactgacctg catcggccca ccggtgcgca ccggattgag cgaaccaaac
     2761 agcgtctgcc ccattggcac catgctgatc agatcgtcaa aaatcgcgct caattgcttt
     2821 tctgtacgca ccgaatctaa ccgttcgcta tagcttttgc cgtttggcga tttgattttt
     2881 agcgcggtat gcaccagaaa agcaggaatg tgcatccgtt cagcacgacg gtcaatctct
     2941 tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt tgactcctgc
     3001 tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc ctgactttca
     3061 aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc
     3121 tgccgcacga cgctcgccac atctatcgct ttttcgccct ttttcaacgg ttgcggagcc
     3181 tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga tagaggattg
     3241 acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg aattttgtca
     3301 ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt
     3361 gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa aacgttagta
     3421 atctgcgtca tcaggccgag tgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac
     3481 aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatattgaa atacatatag
     3541 tgaaaataga ggcggaaata gtttttccgt acggcgctaa aaagctcgcg taccgtaggc
     3601 ggcgtcacgc gcgtggcatc gtcttcacca taaaccagtt ctttacggta ggcagcctct
     3661 acacgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc cagtaatccg
     3721 gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat gtgcccgaca
     3781 atcggcagct ccggcacatg tgcggagagc gttaccagca ccggcaggaa ggcgatcaac
     3841 gtcatgatag cgttgataaa gctgacgccc atattctcca gcgttgaagc aaaacgcatg
     3901 gtgtcttcct gcacacgctg cgcggcccct tcgatatgac gcagttgttg ccagttcgcc
     3961 atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac aaagaagttg
     4021 ttcagcacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg
     4081 taaaattgtt cgatggtgac tttatgcggc gaacttagcg ccgtttgaat cagatcatag
     4141 aacggcgcat accaggcgtt gacggcaacc ccgacttcca ccaaaaacca ggtgacgaag
     4201 ataatcagcg cagtaccgag tattgaccag tattgccaac ggtgcgggct atagataaac
     4261 cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc
     4321 aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt gatacgcgcc
     4381 acccaatcac ccccaccgac ttgccagaag ataacggcaa tcaatgccca aacaaaggcc
     4441 gagagaaaaa acattcccgg ctttgggaaa aaagacttaa acatatgcac tcctgctaac
     4501 ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca
     4561 aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct gaccataaga
     4621 attgtttcga cgggtttcac aaaccaaaga ttgtttacat aagcggatta acacgcgcag
     4681 gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt
     4741 ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag tataaatacg
     4801 ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg cttttttctg
     4861 ccgtgctgta ttcggcgtca ttgacctccg tccatgcggc tcagccgatc actgaaccgg
     4921 agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc agcggtgcca
     4981 cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgcagt tatggcgaaa
     5041 cgcgacctgg taataacgtt cgcccgcagc tggattccgt catacgtatt gcttccctca
     5101 ccaagttaat gaccagtgag atgctggtaa aattgctcga ccaggggacc gtgaagttaa
     5161 acgatccgct aagtaaatat gctccgccag gcgcacgcgt accgacctac aatgggacgc
     5221 ccattacgct ggtgaatctg gcaacccata ccagcgccct gccccgtgaa cagcccggtg
     5281 gcgcggcaca tcgtccggta tttgtctggc caacgcgcga gcaacgctgg aaataccttt
     5341 ccactgccaa actaaaagcc gcgccgggca gccaggccgc gtactccaac ctcgcattcg
     5401 atctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag
     5461 agcagattac ccgtccgttg gggatgaaag acaccaccta caccccctca ccggatcagt
     5521 gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg ctggcggcaa
     5581 ttggcagcgg cggcgtctat tccacgccag gcgatatgat gcgctggatg cagcagtatt
     5641 tgtcatctga tttttatcaa cgcagtaacc aggctgatcg catgcagacg ttgatttatc
     5701 agcgcgcgca gtttacaaaa gtgattggca tggatgtccc cggcaaagcg gatgcgcttg
     5761 gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt cagaagacag
     5821 gtggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat attggtgcgt
     5881 ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg
     5941 acctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc tgattaatac
     6001 tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat atagccgcct
     6061 ttacgcaaag cagcgagaac ttccgcaacg acagaacgcg atatacgtgt acgttgatgg
     6121 atataattca tcacgccaat acgtgatcgc aattcttcat tccagtcaat cattgataat
     6181 aacgtcgcgc ggatttgttc gtaggaatta tgcccaatga gctgaacgtc gcgtaattcc
     6241 agaattctat tttgccaggc taaccagtaa aaagcgtctc gccagagttg attttgttca
     6301 ataagagtaa tggtttgttt ggctggcaaa tgatatcccg tacagtttcc ttctgatatt
     6361 aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat ataaggagcc
     6421 tgtgtaatac cgataagtac gttttcttct ctacgcagag agataacacc ttctaaaata
     6481 acgaaagtat cttcgctatt gacatcactg gaaaatataa cttgcttttc attattaaac
     6541 tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc ctgaagaggt
     6601 ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc
     6661 attatttaga ttaaattacc tatttcttcg gagtgaagaa tattaccagg tatatttaac
     6721 acccacattc gcggaccagt cttgatctac gtcaccacca ccgaggtagt tagcatcggt
     6781 ataggcgctg aagttcttgg tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga
     6841 cccttcagta ccgttatcga tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc
     6901 gtagacgtaa gccagtttga agtacggagt cagagcctgg tcttcgctgt aggtgaaggt
     6961 ataacctgca tctacaccca gttcataacg catgctgtcg taagactgac catcaacttt
     7021 catgttgttg ctcagctggt aatcatcacc agactggaac agaccagaaa cgctgccgta
     7081 aggagttacg taacctgcat cacccagttt gaagtcgtaa ccggctttca aaccaaagcc
     7141 ccaagcgtcg gagttggtgc tgccgtcaac gtaagtaccg ttgctcatgg ttgcagacag
     7201 gtcgttgttg aagtgagagt agctcaagct accgtcaaca aagacgttgt tcgcgaagtg
     7261 agcagaagag tagatgtagg cagtctggct gtcttgatcc acctgaccag aacggtcatt
     7321 catatcacct ttagcgaagc ctgcagccgc accgacgatc cacttagcgt tgttaccgtc
     7381 aattttggta tcaacaccga ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc
     7441 gttgtcgccg ttgaagttac caccgaagta gcttacccat gcgccgccgt tatcagccag
     7501 accatgacga gagttggtca gacgagtacc aacggtgtct tgttccaggt tccagatatt
     7561 ggtgttcgca gacgggatgc tcagcgccat gttagcgtag tcggtcagct ccatctgttg
     7621 cagaacaacg gtgttaccac gctgctcagc ctgataggtg tatgcaccca ggtcagcttt
     7681 gttagcagca gagaaagtcg cgttgctgtt tttgtcgttg acgtagataa tttctttatc
     7741 tttgtaatca gcgattgcgc cagcgccagt tgcgttgtcg atacgaactt tgtagttacc
     7801 ggttgcagca acaacgttgt ctacagaact gttatccagt tcagtaccgt tgtctacgtc
     7861 gccgttgcca ttgatagtca ggtgaccatc agagttcata gcaacaatac cgtaaccgta
     7921 gttagacttg gaagtatccc acgtacggtc gttcaccaaa tccgcgttca gaacgtaatc
     7981 gctgctgttg atgtcgaaca caccagaaac tacgttgccg ttatggtcag cgatgttgcc
     8041 gctagtcagt tccagtttgt cagtacggaa agcttcagta tcaaactgat caacgttcac
     8101 atccagtaca ccaccgttag ttacggtgat ggtattggca tacaggttag aatagttatt
     8161 ccagccagta gattcggtta aattaacggt actgtagcta tcaacagtca ggtggtctgt
     8221 agcaaccaaa ccatcttcac caatattcag tgaggagccg ttggttaaac caatggtatc
     8281 tgagaccaga gaggaatcag taacatttac ctgagagcca ttatttacag ctaatgtatc
     8341 aattactgaa ctattaacgg tattccattc tgaaccattg ttcagtgcta cgttaaatac
     8401 gccgctctgg aataccttgt tgtcaataat acgaccccaa tcgttagctt cgatagtggc
     8461 accaggggtc aggctgttcg gcgcaacatc atacatatct gccggagcaa ttacttcagc
     8521 actcatgttt gctgagccaa cccacttact accgttgttc agagtaatgt tcagttcatc
     8581 aacgttctga gcatcatcta cccaaccacc gttggtatca aggacaccgt caccattgct
     8641 gtcatggccg gttgaataga agaatacgcc atcgttattc cagtggcttg tgaactcgac
     8701 atcacccaac agggttgaat ggtcaaagtt tacattgttt ttcatggaat aatttgagcc
     8761 agcattgtca gagaatgaca gagctacgtc attttcgcca ccattaccag cgtaatcact
     8821 tggttcagca gatttaccga agaagccatt gctttcttcc agcggagtct gtgaccctga
     8881 agttacagtg gagttagaca cattaatgtt gttgtcccac tgatgatatt cgctagtata
     8941 agtcgtgttg cttgtattgc cttcagacag cgcaatacca gctacacggg agttgttagt
     9001 aatattgata ttgctttcaa catcaagatt tactgcaaca cccagagcgg tatagaacgt
     9061 ttcattggtt gacttagatt tatcaccatc aagatatgtg tcagtgaagt aaagagcttc
     9121 atagtcatca tcaatggtgg aattggatac gttcagagtg aatacgtcac catcgtacca
     9181 attgtagtca aggtcagtag tgattccgtt atagaaatta tagccactat aggtctgtgg
     9241 agttacaact tcacctttag cattaacttc tgcaggagta atgctgtaat aaccaaatgg
     9301 cagttcagaa gtgattgacc catgaattac tgaattgctg atatctaact ggttaacaga
     9361 accgctagcg taatcatgat tcaagtaata agtagaaatg gtaccgttaa cagtatcgcc
     9421 tttaaagacg gtgtcgaaat caccattggc attgttatag taaacaaaac cattgtaagt
     9481 tacgtagtta ccgtctgaac ccatcgggtc catgccgcca gcatcggcaa ttccatcatt
     9541 gtcagtgtca tacggccaaa ctaaatcggt cgtggtgttg tcatgatacc cccaggtatc
     9601 atagtttgct gcattagcct gagaggtgat agccagagtg caagccaatg ctaattgtga
     9661 tactacaagt ttctttttcc aggagtgcat ttgtcatccc tcctcaggga cgtaataagt
     9721 tgatccatca attatcaatg catagaaaac atattatgtt tcgcgtttac gattatgcgg
     9781 cgttatagag gaatggttca attattcttt cccctaagtc cgattgcgga catatataaa
     9841 gca
//
LOCUS       DAPQZD010000175         9805 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPQZD010000175 DAPQZD010000000
VERSION     DAPQZD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.177"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            176..1333
                     /gene="acrE"
                     /locus_tag="RO454_004171"
     CDS             176..1333
                     /gene="acrE"
                     /locus_tag="RO454_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDY0935920.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1345..4449
                     /gene="acrF"
                     /locus_tag="RO454_004172"
     CDS             1345..4449
                     /gene="acrF"
                     /locus_tag="RO454_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDY0935921.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4702..4923
                     /locus_tag="RO454_004173"
     CDS             4702..4923
                     /locus_tag="RO454_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935922.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5354..6379
                     /locus_tag="RO454_004174"
     CDS             5354..6379
                     /locus_tag="RO454_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0935923.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6447..7628
                     /locus_tag="RO454_004175"
     CDS             6447..7628
                     /locus_tag="RO454_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDY0935924.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7638..8741
                     /locus_tag="RO454_004176"
     CDS             7638..8741
                     /locus_tag="RO454_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDY0935925.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8749..9507
                     /locus_tag="RO454_004177"
     CDS             8749..9507
                     /locus_tag="RO454_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDY0935926.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(9570..9685)
                     /gene="rrf"
                     /locus_tag="RO454_004178"
     rRNA            complement(9570..9685)
                     /gene="rrf"
                     /locus_tag="RO454_004178"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9725..9800)
                     /locus_tag="RO454_004179"
     tRNA            complement(9725..9800)
                     /locus_tag="RO454_004179"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9765..9767),aa:Thr,seq:ggt)
BASE COUNT         2333 a         2344 c         2519 g         2609 t
ORIGIN      
        1 agtagaatat taatcttttt ttgtgtttat gtgccttgag ttgccagtat tcataactat
       61 tccttacatc gacgaatgat aatttgtagg atagcgaact gtatttttct ttctgcgagt
      121 taacgcattg cctttttggg caaataacgc gcttttggtt ttttgaggaa tagtaatgac
      181 gaaacatgcc aggtttttcc tcctgccctc ctttattctg atctccgcgg ctttaatcgc
      241 cggttgtaac gataagggag aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca
      301 tattgtgaaa acggccccgt tagaagttaa gactgaatta ccaggccgca ccaatgctta
      361 ccgtatagcc gaagttcgcc cacaggttag cgggatcgta ctgaatcgca atttcactga
      421 aggcagcgat gtgcaagcag gccagtccct gtaccagatc gatcccgcga cctatcaggc
      481 aagttatgac agcgcgaaag gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca
      541 tttgacggta aaacgttacg ttccgctcgt gggtacgaaa tacatcagtc agcaggagta
      601 cgaccaggcc attgctgatg ctcgccaggc cgatgccgcc gtgattgccg caaaagccac
      661 agtcgaaagc gctcgcatca atctcgctta taccaaagtg actgcgccaa ttagcggacg
      721 tatcggcaaa tcgaccgtga ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact
      781 ggcgactgtc cagcagctcg atcctatcta cgttgatgtg acccaatcca gcaacgattt
      841 tatgaggctg aagcaatccg tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa
      901 cgtagagttg gtcatggaga acggtcaaac ctatcccctg aaaggtacgc tgcaattctc
      961 cgatgtgacc gttgatgaaa gcaccggctc cataacccta cgtgctgtct tccctaaccc
     1021 gcaacatacg cttttgccgg gtatgtttgt gcgcgcacgg attgatgaag gcgtccaacc
     1081 tgacgccatt cttatcccgc aacaaggcgt tagccgcaca ccacgtggtg atgcaaccgt
     1141 gctgattgtt aacaataaaa gtcaggttga agcgcgccct gtcgttgcca gtcaggcgat
     1201 tggcgataaa tggttgatta gcgaaggtct gaaatctggc gatcaagtca ttgtcagcgg
     1261 cctgcaaaaa gcgcgtccgg gagagcaggt taaagccact accgataccc ccgcagatac
     1321 tgcatcgaag taaggcaatc tgacatggca aactttttta ttcgacgacc gatatttgca
     1381 tgggtgctgg ccattattct gatgatggcg ggcgcactgg cgatcctaca attgcccgtc
     1441 gcccagtatc caaccattgc gccgcctgcg gtttctgttt cggcaaacta tccgggtgct
     1501 gatgcgcaga ccgtgcagga tacagtgacg caggttatcg aacagaatat gaacggtatc
     1561 gataacctga tgtatatgtc ctccaccagc gattccgccg gtagcgtgac aattaccctt
     1621 accttccagt ccgggaccga tcctgatatc gcgcaagtac aggtgcagaa caaactccag
     1681 ctcgccacgc cgttgctgcc gcaggaggtt cagcagcagg ggatcagtgt tgaaaagtcc
     1741 agtagcagct atttgatggt ggcgggcttt gtctctgata accctggcac cacacaggac
     1801 gatatctcgg actatgtggc ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc
     1861 ggtgacgtac agcttttcgg cgcacagtat gcgatgcgta tctggctgga cgccgatctg
     1921 ctaaacaaat ataaactgac accggttgat gtgattaacc agttgaaggt acagaacgat
     1981 cagatcgctg ccggacagtt gggcggaacg ccagcgttac cagggcaaca gttgaacgcc
     2041 tcgattattg ctcagacgcg gttaaaaaat ccggaagaat tcggcaaagt gaccctgcgc
     2101 gtaaacagtg acggctcggt ggtgcgtctg aaagatgtcg cacgggttga acttggcggt
     2161 gaaaactata acgttatcgc tcgcatcaac ggaaaaccgg cggcgggcct ggggattaag
     2221 ctggcaaccg gcgcgaatgc tctcgatact gcgaaagcca ttaaggcaaa actggcggaa
     2281 ttacagccat tcttcccgca gggaatgaag gttctttacc cttatgacac cacgcctttc
     2341 gtccagcttt ctatccagga agtggtgaaa acgctgttcg aagccattat gctggtgttc
     2401 ctggtgatgt atctgttctt gcagaacatg cgggcaacat tgatacccac cattgctgta
     2461 cctgtcgtgt tgttagggac atttgccata ctcgccgctt ttggttactc catcaatacg
     2521 ctaacgatgt ttgggatggt acttgccatc ggacttcttg ttgatgatgc gatagtcgtt
     2581 gtggagaacg tcgagcgcgt aatgatggag gataaactcc cccccagaga agcgacggaa
     2641 aaatcgatgt cgcaaatcca gggcgcgctg gtaggtatcg cgatggtact gtcggcggta
     2701 tttattccga tggcattctt cggtggctca acaggggcaa tttatcgcca gttctctatc
     2761 actattgttt cagcaatggc gctttcagta ctggttgcgc tgattctcac ccccgcgtta
     2821 tgtgcaacat tgctgaaacc agtctctgcc gagcatcacg aaaataaggg aggattcttc
     2881 ggctggttta acaccacttt cgatcatagc gttaatcact acaccaatag cgttggcaaa
     2941 atcctcggtt ccacagggcg atatttactg atctatgcgc tgattgttgc aggaatggtg
     3001 gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg
     3061 accatgattc agttacccgc tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa
     3121 gtgacggatt actatctgaa gaacgagaaa gcgaacgttg aaagtgtctt tacagttaac
     3181 ggctttagct tcagcggcca ggcacaaaac gccggtatgg ccttcgtcag tctgaaaccg
     3241 tgggaagagc gtagtggtga cgaaaacagt gcggaagcag taatccatcg tgccaaaatg
     3301 gaattgggca agatccgcga cggttttgtc attccattta atatgccagc cattgttgaa
     3361 ctaggcacgg caacgggttt cgactttgag ttaattgatc aggctgggct gggtcacgat
     3421 gccctgaccc aggcccgtaa ccagttgctt ggcatggcgg cgcaacatcc tgccagctta
     3481 gtcagcgtgc gccctaatgg cctggaagac accgcgcagt ttaaactgga agttgaccag
     3541 gaaaaggcgc aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacggcg
     3601 ttgggtggga cttacgttaa cgacttcatc gaccgtggtc gcgtgaaaaa ggtgtatgtt
     3661 caggcggatg ccaaattccg tatgctgcca gaagatgtcg ataaacttta tgtccgcagc
     3721 gccaacggcg aaatggtgcc attctctgcc tttaccactt cacattgggt gtatggctct
     3781 ccgcgactgg aacgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca
     3841 ggaaccagtt ccggcgatgc tatggcgttg atggaaaacc ttgcgtcaaa attaccggcg
     3901 ggcattggtt atgactggac gggtatgtca tatcaggaac gtttatcggg aaaccaggct
     3961 cccgctctgg tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa
     4021 agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgcta
     4081 ctggcggcaa cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg
     4141 acaattggct tgtcggccaa aaacgctatt ttgattgttg agttcgctaa agatctcatg
     4201 gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat gcgcctgcgt
     4261 cctatcctga tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac
     4321 ggtgccggca gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg gatggtttct
     4381 gcaacgttgc tggcaatctt cttcgtgccg gtgttctttg tggtgatccg ccgttgcttt
     4441 aaaggataaa tcagaaacat aaaggcactt tcgggtgcct ttattatttc cagtgaaacc
     4501 cataaaaatt aataagatat tcctctactc actttttaaa aactttctat agttcccgct
     4561 cccttcacta tttttacaat tcacataatt tgtcattatt cattccgcaa gatttataac
     4621 ctccagtgat aaaatggcat tcagctcgtt aataagagag ttaacttatt aagcgttagc
     4681 gtttattact gaggtaacac catgaaaaga ttaattcctg tggcattgct caccgcattg
     4741 ctggcgggct gtgctcacga ttcgccctgc gttccggttt atgacgatca gggtcgactg
     4801 gttcacacta atacctgtat gaaaggcacg actcaggata actgggaaac ggctggggct
     4861 attgccggcg gggcagcagc tgttgctggc ctgacgatgg ggatcatcgc tctgtcgaaa
     4921 taactaaaca gggcgcggag ctccgcgctc tttttaacga tacagtatct ttccataaga
     4981 taaaatattg ctatattctg ctgggtaatt ccctgctgat ttagcatgtt atatcacgtt
     5041 atttcgctgc tttcgaaata acgcatcaat ttaactttca cacttcccac cagaaatata
     5101 ctcacgctaa caatcattat tctgcttagt aaaaaacgcg taaatatttt gcgccaaaat
     5161 gtggcgcatg tttcattttc gcaccattac gaggcgctgt ttttattatt tcctgtctac
     5221 actcagctta ttgcgacgcg aaatcgtgcg caaaagctgg cactactttt gcttataaga
     5281 ggatggccac agacaggtaa aagacgtttc ccaaacgtcc tataacgata attttcgcct
     5341 cacaggaagc attatgaaaa agatgatgat agccacactg gctgccgcca gcgtgctgct
     5401 tgccgttgca aatcaggcgc atgctggcgc gacgcttgat gccgttcaga aaaaaggttt
     5461 tgtgcaatgc gggatcagtg atggattacc tgggttctct tatgccgatg ctgacggtaa
     5521 gttttcaggt attgatgtag atgtttgtcg tggtgttgcc gctgctgtat tcggtgacga
     5581 tacgaaagtg aaatataccc cgctcactgc aaaagaacgc ttcaccgctt tacagtcagg
     5641 ggaggtggat ttgctctccc gtaatacgac ctggacttca tctcgcgatg ccgggatggg
     5701 aatggcattt actggcgtca cttattacga cggcattggc ttcctgacgc acgataaagc
     5761 ggggctaaaa agcgcgaaag aactggatgg cgctaccgtc tgtattcagg cggggactga
     5821 taccgaactc aacgtcgccg attacttcaa ggcaaataat atgaagtaca cgccagtaac
     5881 cttcgatcgc tcagacgaat cagcgaaggc tctggaatct ggccgctgtg atactctggc
     5941 ctcggatcaa tcacaactgt atgccctacg catcaaatta agcaacccaa ctgaatggat
     6001 tgtcttaccg gaagttatct ctaaagaacc acttgggccg gtagttcgtc gtggcgatga
     6061 tgaatggttc tcgattgtac gctggacgct tttcgccatg ctgaatgctg aagagatggg
     6121 catcaattcc cagaatgtcg atgaaaaagc ggctaatcca gcaacgcctg atatggcaca
     6181 tctgctgggt aaagaaggcg attacggcaa ggatctgaag ctggataata aatgggccta
     6241 taacatcatc aaacaggtgg gtaactactc ggaaattttc gagcgtaacg taggttcaga
     6301 aagcccgctg aaaattaaac gtgggcaaaa taatctctgg aataacggcg gtattcagta
     6361 cgcgccgccc gtgcgttaag tcggtgtgag gtaacgagcg ttgctacggc ggcgctcttt
     6421 tcagcgttat ggttttcgag gtttctatgc cccatcgccg ctcaaccgtt aaaggctcac
     6481 tctcttttgc caaccctacg gttcgcgcct ggttattcca gatccttgcc gttgttgctg
     6541 ctgtcagcat tgttggttgg ttatttcaca acactgtaac gaatctcagt aatcgtggca
     6601 taacatcagg atttgctttt cttgaccgcg gggccggttt tggcatcgtt cagcatttga
     6661 ttgattatca gcaaggcgac acttacggac gcatttttat tgtcggccta ctcaatacgc
     6721 tactggtttc tgcattgtgt attgtgttcg cttctgtttt gggcttcttt atcggtctgg
     6781 cgagactttc ggataactgg ctgctacgaa agctttccac aatttatatt gagatcttcc
     6841 gtaatattcc cccgctgctg caaatcttct tctggtactt tgccgtgttg cgcaatttgc
     6901 ccggaccacg acaggcagtg agcgcgtttg acctggcctt tttgagcaat cgtgggcttt
     6961 atattccgtc gccgcagctg ggagacggat ttattgcgtt tatcctggct gttgttatgg
     7021 ctatagtcct ttctgttggg ctattccgct ttaataaaac ataccagata aagaccggac
     7081 aactgcgccg cacctggcca ttcgccgctg tgttgatcat tggtttgcct ttactggcgc
     7141 aatggctttt tggcgcagca ctacactggg atgtcccagc ccttcgaggc tttaatttcc
     7201 gcggcgggat ggttttaatt cctgaactgg cagccttaac gctagcactt tcgatttata
     7261 cctctgcatt tatcgccgag attatccgcg cagggatcca ggcagtgcct tatggtcaac
     7321 atgaagcggc tcgatcactg ggattaccca acccggttac gctacgccag gtcattattc
     7381 cccaggcatt gcgggtgatt attccaccgt taaccagcca gtatctcaac atcgtcaaga
     7441 actcctctct tgccgccgct attggctatc ccgatatggt ttcgctgttt gctggcaccg
     7501 tgctgaacca aacggggcaa gccatcgaga cgatagccat gaccatgtcg gtctatctga
     7561 ttatcagcct gactatctcg ctgctgatga atatctataa ccgccgcatc gcgatcgttg
     7621 aacgctaagg agtcatgatg acaaaagtat tgctgtctca tcccccgcgc ccggcgagcc
     7681 ataactcaag ccgcgcgatg gtgtgggtgc gaaaaaatct gttctccagc tggagcaata
     7741 gcctgctgac tattggctgc atatggttga tgtgggaact gatcccaccg ttgctgaact
     7801 gggcattttt gcaggctaac tgggttggct caacgcgtgc cgactgcaca aaagccggtg
     7861 cctgttgggt cttcatccac gaacgatttg gtcagtttat gtatggactt tacccacacg
     7921 accaacgctg gcgaattaac ctggcattac tgattggcct tgtgtctatc gcaccaatgt
     7981 tctggaaaat acttccgcat cgcggtcgct atattgcggt ctgggcggtg atttacccac
     8041 tggttgtctg gtggctgatg tatggtgggt ttcttggtct tgatcgggtt gaaacccggc
     8101 aatggggcgg gttgacgcta actttaatta ttgcatcagt tgggattgcg ggggcgctac
     8161 cgtgggggat cttactggcg ttaggtcgcc gctcccatat gccgattgtg cgtatcttat
     8221 cggtcatttt tatcgagttc tggcgcggcg taccgctgat taccgtattg tttatgtctt
     8281 cggtcatgct gccgttgttt atggcagaag gcaccagtat cgataaactg atccgcgcgc
     8341 tggttggcgt gatcctgttt cagtcagcat atgttgcgga agtcgtgcga ggcggattac
     8401 aggcgctgcc taaagggcaa tatgaagcgg cagagtcgct ggcattgggt tattggaaaa
     8461 ctcaggggct tgttattctg ccccaggcgt tgaagctggt aattcctggg ctggtaaata
     8521 ccatcatcgc actcttcaaa gataccagtc tggtgatcat catcgggttg ttcgatcttt
     8581 tcagtagcgt tcagcaggca accgttgatc ccgcgtggtt gggtatgtcg acggaagggt
     8641 atgttttcgc cgcgctgatc tactggatct tctgtttcag catgtcacgt tatagccaac
     8701 atctggaaaa acgttttaac accgggcgta caccgcattg aggacactat gagccaaatt
     8761 ttactgcaac ctgctaacgc gatgattacg ctggaaaacg tcaataaatg gtatggacaa
     8821 ttccatgttt tgaaaaatat aaatttaacc gtgcaaccgg gcgaacggat cgttctatgt
     8881 ggcccttcag gttccggtaa atcaacaacc attcgttgta ttaatcatct ggaagaacat
     8941 caacagggac ggatcgtggt agatggcatc gaacttaatg aagatatccg caatattgag
     9001 cgcgtcaggc aggaagtggg aatggtcttt cagcatttca atctcttccc tcatctgacc
     9061 gtgttacaga actgtaccct ggcaccgatt tgggtacgca agatgccaaa gaaagaggct
     9121 gaagctctgg cgatgcatta ccttgagcgg gtgagaattg ccgaacatgc ccagaagttt
     9181 cccggacaga tttctggtgg tcagcagcaa cgtgttgcca tagcacgttc gctgtgtatg
     9241 aagccgaaaa ttatgttgtt tgatgagcca acgtcggcgc tcgatcctga gatggtgaaa
     9301 gaggtgctgg atacgatgat tgggctggcg cagtcgggta tgacaatgtt gtgtgtaaca
     9361 catgagatgg ggtttgcacg aaccgtcgct gaccgggtga tttttatgga tcgtggggaa
     9421 atagtggagc aagctgcacc tgatgaattt tttgcgcatc ctaaatcaga gcgtacgagg
     9481 gcatttttat cgcaggtaat ccattaattg aatattagtt cgaaaagcaa aaaggccatc
     9541 ctttcggatg gcctttcgcc tgatttgatg tctggcagtt ccctactctc gcatggggag
     9601 accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg
     9661 ggaccaccgc gctactgccg ccagacaaat tcttttctaa tctgccgaac tttaacctaa
     9721 aaagtggtgc tgatacccag agtcgaactg gggacctcac ccttaccaag ggtgcgctct
     9781 accaactgag ccatatcagc acgct
//
LOCUS       DAPQZD010000176         9645 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPQZD010000176 DAPQZD010000000
VERSION     DAPQZD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.178"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..352)
                     /locus_tag="RO454_004180"
     CDS             complement(<1..352)
                     /locus_tag="RO454_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDY0935927.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRND"
     gene            593..886
                     /locus_tag="RO454_004181"
     CDS             593..886
                     /locus_tag="RO454_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDY0935928.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            958..1617
                     /locus_tag="RO454_004182"
     CDS             958..1617
                     /locus_tag="RO454_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDY0935929.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1709..2155
                     /locus_tag="RO454_004183"
     CDS             1709..2155
                     /locus_tag="RO454_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDY0935930.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2362..2751)
                     /locus_tag="RO454_004184"
     CDS             complement(2362..2751)
                     /locus_tag="RO454_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HDY0935931.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETARFYVDYDDLMLSLLKE
                     AAQKMINTCNEYQKRHGKKTLFEIPEV"
     gene            2948..3367
                     /locus_tag="RO454_004185"
     CDS             2948..3367
                     /locus_tag="RO454_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDY0935932.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3367..4635
                     /gene="umuC"
                     /locus_tag="RO454_004186"
     CDS             3367..4635
                     /gene="umuC"
                     /locus_tag="RO454_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDY0935933.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(4680..5210)
                     /gene="dsbB"
                     /locus_tag="RO454_004187"
     CDS             complement(4680..5210)
                     /gene="dsbB"
                     /locus_tag="RO454_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDY0935934.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5356..6897)
                     /gene="nhaB"
                     /locus_tag="RO454_004188"
     CDS             complement(5356..6897)
                     /gene="nhaB"
                     /locus_tag="RO454_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDY0935935.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7119..7838
                     /gene="fadR"
                     /locus_tag="RO454_004189"
     CDS             7119..7838
                     /gene="fadR"
                     /locus_tag="RO454_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDY0935936.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(7890..9422)
                     /locus_tag="RO454_004190"
     CDS             complement(7890..9422)
                     /locus_tag="RO454_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDY0935937.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
BASE COUNT         2527 a         2287 c         2408 g         2423 t
ORIGIN      
        1 cgtcgtttcg ggtttgcagc actcgcagct catatttgcc agaagcaggg agtgagtatt
       61 gtccgtgact atcgagttct ggggaatatc tggataaatc aacagaatca tcgattcctg
      121 gcccgaagag gtaagtatca gcgccttcat tagagatact cacatgcact ttctgacctt
      181 ttttggcata gaaggtatat gtatcgtaat cgtatccctt tatttcgccg gagtactgag
      241 cgctactatg gccttttctg aactcaacat tgacattctt acccgcagca aagctcgtgg
      301 aagttagcag tgcaagcaac aatacagcct tactgatgcc cttaattttc attggtagtc
      361 tcctgttcag aatgagtacg atgaactgtt ataatataac aatccttcac gggagaattc
      421 cagtggcttt cctgtgttcg gttaaatcct ggcgcggcat ttttgccggg ggattactta
      481 taaagggggc atggactgaa caaaccgtag aaatctacag ctggctggct tgtggcgcgg
      541 gtttcattga tgttagctta tgccaaaacc tggcatctta aagagtaaaa gtatgttttg
      601 tgtgatttat cgaagcagca aacgtgacca gacctattta tatgtcgaaa aaaaagacga
      661 tttttcgcgt gttcctgagg aactgatgaa aggttttggt cagcctcagt tagcgatgat
      721 tcttccgctg gatgggcgga agaaactggt caatgccgat attgaaaagg ttaaacaggc
      781 attaaccgag caaggttact atttgcagtt accgccacca cccgaagatt tgctgaagca
      841 acatctttcc gtcatggggc agaaaacaga cgacactaac aaataaccga tatccggcgg
      901 tggcattatc tttgtcggcg cgggttttca tatccacgat aaggtgaggg gaacgttatg
      961 tatcaacatc acaactggca aggtgcgctg ctggattatc cggtgagtaa agtagtctgt
     1021 gttggcagta actatgccaa acatattaaa gagatgggca gcgcagtacc cgaagagcca
     1081 gtgctgttta ttaaaccaga aacggcactg tgcgatctgc ggcagccatt ggcgatccca
     1141 tccgatttcg gttcagttca tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg
     1201 cgtcaggcta cggaagagca tgtccgcaaa gccattgccg gttacggcgt ggcgctcgat
     1261 ctgacattgc gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggcc
     1321 aaagcgtttg ataactcttg tccgctttcc gggtttattc ccgcggcgga atttaccggc
     1381 gacccgcaaa atacaacgct gggcctgagc gtaaacggcg agcaacgcca gcaaggtacg
     1441 actgcggaca tgatccataa aatcgttccg ctgatcgctt atatgagcaa gttttttacc
     1501 ctcaaggtcg gtgacgttgt gctgacaggc acgcctgatg gtgtcggccc gttgcaaagc
     1561 ggtgatgagc tgacagtcac tttcgatggg cattctttga caactcgcgt tttgtaatac
     1621 tttttgccgc ctgaaagcgg cggcaaaact tgcatcgctg tgccagactg gttataaggt
     1681 gcgttttaac gtaatggcgg aacacctgat gagcgatgta cctttctggc aaagtaaaac
     1741 cctggacgaa atgagcgatg cagaatggga gtcgttgtgt gatggttgcg gtcagtgttg
     1801 cctgcataaa ctgatggatg aagacaccga cgaaatctac ttcactaacg tcgcctgtcg
     1861 ccagctcaat attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc
     1921 cgactgcatt aaattaaccc gtgaaaatct gccaacattc gaatggctgc caatgacctg
     1981 cgcttatcgt ttgctggcgg aaggtaaagg tttacctgcg tggcatccgc tacttactgg
     2041 ttcgaaagcg gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc
     2101 agaagtgatt gactggcagg atcatatctt aaataaacct gactgggcgc agtgataaat
     2161 ttaatagatg ttgatggggg ctttcttaat atacgtctgg atgctcgcca tttcgtagcc
     2221 ctgtttttga aagcgctctg tcataatgag agttacccga tactttatta tcaacaggta
     2281 actctcaatc tttaaaaaac gtctgtattt ttaaatgtga taaattttat accttgagta
     2341 catagagaga ataatcgctt atcagacttc aggtatctca aagagtgtct tcttaccgtg
     2401 tcttttctga tactcattac aggtgttaat cattttttgg gccgcttctt ttagtaaaga
     2461 aagcattaaa tcatcataat caacgtagaa tctggctgtt tcagtttccg tttttatctc
     2521 cccgatggcg gctatttcgg tggttaattt caatttggcg gcatcgatat ctttattcgc
     2581 ttgtttaacc gtgttagaca gggtggtaaa gaaactctgc acagatttta acttgttctt
     2641 caattctgga atcagtttcc cttcaactac gcccgcagca atagaacaca gtttcttgat
     2701 gccgtcatcc agtaccttaa tgagaatgcc tttctgggcg aatgcttcga ttctatttta
     2761 aaccaggtga tatttttcct ttctgctgga tggacgctgt cgccagaagg ccgcgtgagc
     2821 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     2881 cagtattgat ctgctgacaa gaacagacta ctgtatataa aaacagtata acttcaggca
     2941 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     3001 tagcgatctt gttcagtgtg gctttccttc accggccgca gattacgttg aacagcgcat
     3061 cgatctgaat caactgttga tccagcatcc cagcgcgact tatttcgtca aagcaagtgg
     3121 tgattctatg attgatggtg ggattagtga cggtgattta ctgattgtcg atagcgctat
     3181 taccgccagc catggtgata ttatcatcgc tgctgttgac ggcgagttta cggtgaaaaa
     3241 attgcaacta cgcccgacgg tacagcttat tcctatgaac agtgcgtact cgcccattac
     3301 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     3361 gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     3421 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
     3481 gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg gcgatccctg gttcaaacaa
     3541 aaagatctgt ttcgtcgctg tggcgtggtt tgctttagca gcaattatga gctttacgca
     3601 gacatgagta atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
     3661 agtattgatg aggcattctg cgatctgaca ggtgtgcata attgtcgcga tctgactgat
     3721 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtgtgggg
     3781 atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg
     3841 cagacgggtg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt aatgtctgct
     3901 ctccccgtgg atgaagtctg ggggattgga cggcggatca gcaaaaaact ggacgcgatg
     3961 gggatcaaaa ccgttctaga tttggcagat tcagatatcc ggtttatccg taaacatttt
     4021 aatgtcgtgc tcgaaagaac ggtgcgtgaa ctgcgcggtg aaccctgttt gcaactggaa
     4081 gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga acgcatcacg
     4141 gattatccgt cgatgcggca ggccatttgt agttacgctg cccgggcggc ggaaaaactt
     4201 cgcagcgagc atcaatattg tcggtttatc tccacgttta ttaagacgtc accatttgcg
     4261 ttcaatgaac cttattacgg caatagcgca tcggtaaaac tgctgacgcc cactcaggac
     4321 agcagggata tcattaacgc cgctacgcga tctctggatg ccatctggca agcgggccat
     4381 cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt cgcgcagctc
     4441 aatttattcg atgacaacgc accacgcccc gggagtgagc aattgatggc ggtaatggat
     4501 acgctgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg gatccagcaa
     4561 caatggcaga tgaagcgtgc catgctttca ccatgttata caacgcgaag ttctgattta
     4621 ctgcgggtca aataaacata gcggcaggaa aaagcgctcc cgcaggagcg ccgaatggat
     4681 tagcgaccga acaggtcacg ttttttcgct ttaaacggct gggaaatcac caccagcacc
     4741 gcgacaatca ggtaagcgat aaaaataccg agcagccact gcggcatttc cagacctaaa
     4801 aattcccact gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg cacccattta
     4861 tccagcggca gccattccgg gaaacgagcc ataaaatcac aggtcgcaaa cggcgaagga
     4921 tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc actatacaac
     4981 cagataacca tcgctacata acgcagcgga gttttcgggg cgatcgcgcc aatcagcgca
     5041 gcacccagaa cgccgaataa cgcgcagcgt tcataaatac agagcacgca aggtttcagt
     5101 aacatcacat gctggaacca cagcgccgtc agttccagtg ccagagcagt aaacgccatc
     5161 aacagccatg cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat aatcatttcc
     5221 ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca ccaggaggca
     5281 gcaaaaatag gacgcaattg gacaattatc gggcgaaaag gcaaaccggg catttatatg
     5341 cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc attcggttac
     5401 aggagcaagc gtaaactcga cgcagagtaa cccggcaacc gtcagtacga gggtgtaagg
     5461 cagggccatc cacaccatgc ggccataaga gagacgaatc aatggcgcga gtgcagaggt
     5521 cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acggagggca gattggtacc
     5581 ggtattaatg gcgaccgcca gcagctcata ttgcttcaac gtgatagcgc cactttccat
     5641 tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg aaatggatga
     5701 cagcagaccg ttgaaaatat agaacaacga cagctgagca tgttcagatg cctgcaacac
     5761 aaactgaata atcggcgaaa acagttgttg gtcgataatc accgcgacta ctgagaaaaa
     5821 caccgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga tagcatgttc
     5881 atcggtaaca ccggtcaatg atgtcgccag aataatgact gacaaaccaa tcaaaccaac
     5941 ttccgccaga tgcagtgcca gcgcagtcac cagccagacg ccgataatcg cctggacaat
     6001 cagacggatt ttatcctgac gggtacgctg gttgcggctt tgatcgtcaa actgttgcag
     6061 tacttcgcgg actttctccg gcagcgtttc accgtagcca aaccaacgca gcttctctac
     6121 cagcaggcag gttaacaggc cacaaatcag aaccgggacg gtcaccggcg acatgcgcag
     6181 gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg atcaggttct gtggttcgcc
     6241 caccatggtc atcacgccgc ctaatgcggt gccgacaccg gcatgcatca tcagactacg
     6301 cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat gactatcgtc
     6361 ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg tgataaatac cataaaaacc
     6421 gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg cggagaggag
     6481 cgccgccgcc acgcaaaaag agagcgacag cagcattttg gagcgaatgc ttaacagcaa
     6541 acgggtaaat atgaacaaca acagctgttt cataaaatag atacccgcca ccataaacat
     6601 cagtaacagc aagacttcaa gatttgccgc cacttcttca cgaacatgtt ccgcgctggt
     6661 catgccgatg aataccgctt cgatagccaa cagaccaccg gggagcagcg ggtagcattt
     6721 cagggccatc gccagagtga aaataaattc cgctaccagc aaccagccag cgacgaaagg
     6781 gctgatgagg aaaattaacg gatttacgat taagaaaatg atgagggcga gtttgtacca
     6841 gtcgggggac tggcccaaaa aattgcgcca tagtgcgcgg ccccaggaga tctccatgat
     6901 ggtttccctt accttagaaa taatcaatga tgtttttatg tttaaacgca aagcttaacg
     6961 gtcagacagg agtgaggcaa gtcttgatag tcaaggggaa agagatgcag aaaatgaagc
     7021 cttgatccct tttttcttct ttttgtcttc tatcagcgta gttagccctc tggtatgatg
     7081 agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag gcgcaaagcc
     7141 cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc ttccctcccg
     7201 ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg cgtactacgt
     7261 tacgtgaagt gttacaacgt ctggcacgag atggctggtt gaccattcaa cacggcaagc
     7321 cgacgaaggt gaataatttc tgggaaacct ctggtttaaa tatccttgaa acactggcgc
     7381 gactggacca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg cgtaccaata
     7441 tttcaactat ttttattcgc accgcgtttc gtcaacatcc cgataaagcg caggaagtgc
     7501 tggcaaccgc taatgaagtg gccgatcacg ccgacgcctt tgccgagctg gattacaaca
     7561 ttttccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc cttaacggga
     7621 tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa gcgcgcagtc
     7681 tggcgctggg cttctatcac aaactgtcgg cgttgtgcag tgaaggcgca cacgatcagg
     7741 tatatgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac cggatgcaga
     7801 aaaatctgcc gggtgattta gccattcagg gacgataatc ccttccgttt aaaaagcaaa
     7861 cccctcaaac gaggggtttt tcgttgtttt tacagatttc ccattctcgg cgggcaacgt
     7921 tccagcaact cgatgctgcc gtcttcgttt tgctgttcca gcatcacatc aaatccccac
     7981 aggcgatgca catgcttcag cacttctttg cgcccccgat ccaacggtgc gcgattatgt
     8041 ggaatgtaac gcagcgtcag cgaacggtca ccgcgcaaat ccacgttcca gatctgaata
     8101 ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat ttcccgataa
     8161 ccttcttcat tatgaatagc ggaaatctcc agataattat gtcgatcatc atccagtacg
     8221 gtgaagaagc ggaaatcacg catcactttc ggtgacagga actggctgat aaaactctca
     8281 tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc ggcgatatcc
     8341 gggaaccagt atttgtcttc ttccgtcggc gactgacaaa tccgtttaat atcctggaac
     8401 atggcgaacc cgagggcata tgggttgatg ccgctgtacc acgggctgtt atagggcggc
     8461 tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc cgttactttc
     8521 ccttcatcat acagatggtt aaggatggtg tagtgccaga aggtcgccca gccttcgttc
     8581 atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac aatacgcagg
     8641 atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata gagcaggttt
     8701 tcttgtggtt cggacggata gcggcgcgct tcagcaaccg ttttctcttc ctcgcgcttc
     8761 ggcagggtac gccagagcat attgacctga ctttgcagat actcttcgcg actcttctgc
     8821 cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc cacgccgtag
     8881 ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc ataacgttct
     8941 tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga actggcgtcg
     9001 gtccagctac ggaataagta attgtttttg aagaaagagt tatgcccata gcaggcatga
     9061 gccatcacca acgcttgcat ggtaatggtg ttctcttcca tcaggtaagc gatacacggg
     9121 ttagagttaa tgacgatttc ataggccagt ccttgctgac cgtgcttata cagccgttca
     9181 gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat gccgacgctg
     9241 gagtaagcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg gtaggtatcc
     9301 agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag caggtcgaac
     9361 gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc gatcgtcgcc
     9421 atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt caagcataga
     9481 acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct tattatcaga
     9541 gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg ttcagcagga
     9601 gatactaaag acgccatatt gccgcagagt cagggagatg tgagc
//
LOCUS       DAPQZD010000177         9644 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPQZD010000177 DAPQZD010000000
VERSION     DAPQZD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.179"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            27..527
                     /gene="tssB"
                     /locus_tag="RO454_004191"
     CDS             27..527
                     /gene="tssB"
                     /locus_tag="RO454_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDY0935938.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            586..2124
                     /gene="tssC"
                     /locus_tag="RO454_004192"
     CDS             586..2124
                     /gene="tssC"
                     /locus_tag="RO454_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDY0935939.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2142..3479
                     /gene="tssK"
                     /locus_tag="RO454_004193"
     CDS             2142..3479
                     /gene="tssK"
                     /locus_tag="RO454_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDY0935940.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3476..4141
                     /gene="tssL"
                     /locus_tag="RO454_004194"
     CDS             3476..4141
                     /gene="tssL"
                     /locus_tag="RO454_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HDY0935941.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            4154..5806
                     /locus_tag="RO454_004195"
     CDS             4154..5806
                     /locus_tag="RO454_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDY0935942.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5864..6355
                     /locus_tag="RO454_004196"
     CDS             5864..6355
                     /locus_tag="RO454_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDY0935943.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6546..9182
                     /gene="tssH"
                     /locus_tag="RO454_004197"
     CDS             6546..9182
                     /gene="tssH"
                     /locus_tag="RO454_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDY0935944.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLGWDEEDGIVLEFDEK"
     gene            9194..>9644
                     /locus_tag="RO454_004198"
     CDS             9194..>9644
                     /locus_tag="RO454_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDY0935945.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT         2156 a         2712 c         2792 g         1984 t
ORIGIN      
        1 aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa gttcccgccg
       61 ctcgtgtaaa tatcaagctt gacctgcata caggcaatgc taaaaagaaa gttgaactcc
      121 ccctcaagct tcttgccgta ggcgattaca gtaacggaaa agagcagcgt ccgctgtccg
      181 aacgggacaa gattgatatc aataaaaaca acttcaacag cgtcatggct gagttttcgc
      241 ctgcggttaa tttaacagta gaagatacgc taaacggaaa cggtaatgaa caaaatatcg
      301 cgcttgaatt taaaagcctg aaggattttg agcccgaaca ggtagcaaaa aatattccac
      361 aacttcgtgt attactcgct atgcgcaatt tactacgtga cctgaaatcc aatctgctgg
      421 ataacgcaac gtttcgccgt gaactggaaa atatcctgaa agacccgacg ctcagttcag
      481 aactgcgtga tgaactggcg aaaattgcgc cgcaggagaa cgtttaaccc gaacgcgtat
      541 tcaggttaaa ccggagaaga aaggatcact gaaaaggaaa tgctgatgtc tgtacaacaa
      601 gaacatgcca cctctgaaac tgcaacactc accaccactg agtccggcgg cgtttatcag
      661 tccctgtttg ataaaattaa tttaaccccg gtctcttcca ttcaggaaat cgattcatgg
      721 caaaacagcg aaacgctggc cgatgcctca cccgatgagc gcgtgacggc ggctatccac
      781 gttctgcttt cctgtctggc gaagtctggc gaggacgtgg ttaaactcga caaaagcctg
      841 ctggattttc atatcgacga tctggatcag aaaatcagta aacagcttga tgcagtcatg
      901 caccatccgg aattccagaa agtcgagtcg ctctggcgcg gcacatggtt cgtcgtacag
      961 cgcactgatt ttcgcaaaaa tgtcagaatt gaactgctgg atgtcagtaa agaacatctg
     1021 cggcaggact ttgatgattc tccggaaatc attcaaagtg gtttatatcg ccatacatac
     1081 attcaggagt acgatacgcc gggtggcgaa cctgtagcct cattaatatc cagctatgaa
     1141 ttcgataaca gcccgcagga tattgccctg ctgcgtaata tttccagagt ctctgctgca
     1201 tcacatatgc cctttatcgg ttccgtcgga ccgaagttct tccttaaaaa ctcgatggaa
     1261 gaagtcgccg caattaaaga tatcggcaac tattttgacc gcgcggaata tatcaaatgg
     1321 aagtcgttcc gcgataccga cgacagtcgc tacgtgggat tagtgatgcc gcgcgtgctg
     1381 ggccgtctgc cctatgggcc ggacacagtg ccggtacgca gctttaacta tgtggaagaa
     1441 gtcaaaggcc cggatcacga aaaatacctc tggacaaacg cctcgttcgc ctttgccgcc
     1501 aatatggtga agagctttgt gaataatggc tggtgcgtgc agatccgtgg tccgcaggcg
     1561 ggtggcgcag tggccgatct gccgatccat ctttacgatc tcggcaccgg caatcaggtc
     1621 aaaattccgt ccgaagtgat gatcccggaa acccgcgaat ttgaatttgc caaccttggc
     1681 tttattccgc tctcttatta taagaatcgc gattacgcct gcttcttctc agcgaactct
     1741 gcccagaaac cggcgttgta tgataccgct gacgccaccg ccaacagtcg tattaacgcc
     1801 cgtttgccct atattttcct gctgtcccgc attgcgcatt acctgaaaat catccagcgc
     1861 gagaatatcg gtaccaccaa agaccgccgc gtactggaac tggagcttaa cacctggatc
     1921 cgcacactgg tgacggagat gaccgatccg ggtgatgaac tgcaggcgtc tcacccgctg
     1981 cgcgacggca aggttatcgt ggaagatatt gaggacaatc cgggcttctt ccgcgtcaga
     2041 ctctttgccg tgccgcattt ccagattgaa gggatggata tcaacctgtc actggtttcc
     2101 cagatgccga aagcgaaagc ctgaccgggc cgtggtgtga tatgaaaatt catcgaccac
     2161 tgtgggctga ggggacattc ctgtcctctc agcagtttca gcagcaggcc cgctgggaag
     2221 cgttcagcaa tgacagcatt gcgcaacttt gtattcgtca tccgtggggg atcgcgaatg
     2281 tgctctttga ccgcgatgcc ctgacgtccg gaaagctgaa aacgcaggct gtacgtctgc
     2341 gttttgctga tggcacgctg atcgattcag acgtgagtga cgttctgccg ctcgcctgcg
     2401 atctgcgcgc gctgaagaac gacagcgcga ttgtcctgct ggcgctgccg cttgcgcatg
     2461 gtaacggcgg taatctcggt cagggtgagc agactgaaag accgctgcgc tatcgtcagg
     2521 aatggcaaaa ggtgcaggat atctacggca gtgacagtga agatatggcc gttgagcgtc
     2581 atgcgctgtc cctgcgcttt gctcatgata acaatcagga ttacatcacc tgcccactgg
     2641 cgcgtctggt gcgggatgtt cagggcaact ggacacagga tgagagctat attccgccgt
     2701 tactggcgtt taacgcccat gacgggctgg tacagcgtct ggacacgctg ctgttgcagc
     2761 tacgggcgaa gtgccagcgt ctgatggcga tgcgccggga aagcaaccag cgtatggcgg
     2821 acttcgctgt cgccgatgtc tccctgttct ggctgcttaa cgcgctgaac tccgccgagc
     2881 cggtactgag tgatttcctg cgctacccgg cggttcatcc cgaactggtc tggcgggagc
     2941 tggcgaggct tgccggtgcg ctgctgacgt tttcccttga acataacgtc agcgccgttc
     3001 cgccctatgt ccacgaatcg ccgtccatcg tctttccgcc acttttctcc ctgctcagcg
     3061 aactgctgga agccagcctg ccgtcacggg tgatcgcgct ggatctggcg tccctgccgg
     3121 gcaaccgctg gaaagcagac ctgcacgatc cgcgcctgcg ggaagaggcc gacttttacc
     3181 tgtccgttcg ttcctctctg cctgcgcatc aggtactgca ccagctaccg ctggtctgca
     3241 aaatcggtgc gcctgatgac gtcacgctgc ttatcaatgt ggcgctgaat ggtgttccac
     3301 tggtgccgct tacctcagtt ccggcagcgc tgccactgcg gctggagaac cagtattttg
     3361 cgctggatat gcacagtgac gcggctaaat cgatgctgga gtccgggtgc tgcatgattt
     3421 atgcgccggg aacaatgggc gacctgaaac ctgaactgtt tgcggtgctg cgcacatgaa
     3481 aaaagagacg gatatcaata tcgacgcgct gctgcgcgac acgtttctga cggtggtgga
     3541 actgcgtcag ggaaccaccg tgcgtcacgg tatggagctg tatcgccact gccagcggca
     3601 ggtggaactg gtccgcgaac gcctgaaaga tgccgggttc agccgggaga gcgtggagca
     3661 cattacttac gcgcagtgcg ccctgctgga cgaaacggtc ctgagccgtg gcggcatgga
     3721 cgacggtcag gccatctgga tgaaggatcc actccagtcg catttcttca acactctgca
     3781 ggccggtgag ctgctgtacg agcggatgaa gcaggtgcta caggagcccg cccccgcgca
     3841 ggccgtgctg acctgctttc accgtgtcct gctgctggga tttcgcgggc gttatcagga
     3901 tccggcagcc ccggagcgcg accagcttat ttcgacgctc aacgggcagg tcgcgccatt
     3961 tggtgtgctg ccggaaacgg cggtactgaa cgtgccgtta tccccccgtc agcacccttt
     4021 gctgcattcg ccgtttttct ggctggtgac gctcacactg gtactggccg gtgtctggtg
     4081 ggggctacat tactggctga acgtgctggt ggatgaactg ctgccgcaaa gtctgcgtta
     4141 aagggagtcg tgagtgagga atgcgctgaa acagacggtt ttgttatgga ctctgttgtt
     4201 gctgctgact acctggctgg gtttctttgc tgtatctgcg gtcgtgaagt atgccgtggt
     4261 agtgattctt gtcgccgtca cagcggcggt gatggcgtac tggcataaga aaaagggaca
     4321 ggtcgtagcg gacgacgtat ggccgtcatc gctaccgccg gaaacctacc gacagccggt
     4381 agtgctggtc tgtggcgata tgtcggcgca cctgttcact gacagcccgg tccggcaggt
     4441 ttcagaaggg ctgtatctgc ctgtttctga tgaagaacag cttgtggcgc aggtggagcg
     4501 attactgacc cttcgcccgg cgtgggcatc gcagtttgcc gtggcgtata ccgtcatgcc
     4561 cggcatgtac cgggatgcgg cggttctgac cggacaactg cgccggttcg ctcacagtat
     4621 ggcgacggtg cgtcgccggg cgggcgtaaa cgtaccctgg cttctctgga gcgggctgtc
     4681 cggctcgccg ttgccggaga gagcgaattc accgtggttt atctgtaccg gcggcgaagt
     4741 tcaggtagca acatccgcag agaccaccat gcccgcgcag tggattgcac aatccggcgc
     4801 acaggaacgc agtcagcgac tctgttacct gctgaaagcc gaaagcctga tgcagtggct
     4861 ggatctgaat gtgctggcgg aactgaacgg cccggaggcg aaatgtccac cactggcgat
     4921 gaccgttggg ctggtcccct cgttgcctgc ggtggataac aacctgtggc agttgtggat
     4981 caccgccaga accggcctga cgccggatat cgcggacacc ggcacagacg atgcgctgcc
     5041 attcccggat gccctgttac ggcggttgcc gcgtcagtcg ggctttaccc cgctgcgacg
     5101 ggcctgcgtg accatgctgg gcgtcaccac cgtggcgggt atcgccgcgc tgtgcctgtc
     5161 agccacggca aatcgccagt tattacggca ggtcggtgac gatctgcacc ggttttatgc
     5221 cgtcccggcg gaagaattta tcaccaaagc ccgtcacctg tcggtgctga aagacgatgc
     5281 ggtcatgctc gatgggtatt accgggaagg agaaccacta cgtctcggtc tggggttata
     5341 ccccggtgaa cgcatccgcc agccggtatt acgcgccatt cgcgactggc gtccgcctga
     5401 gcaaaaaatg gacgtgacgg cttcgcttcc ggttcagacc gtgcgtcttg acagtatgtc
     5461 gctgtttgac gtcggacagg cccgcctgaa agacggctcg acaaaagtgc tggtggatgc
     5521 gctggtgaac atccgggcaa aaccgggctg gctgatcctc gtggccggat ataccgatgc
     5581 caccggcgat gaaaaaagca atcagcagtt atcgctgcgg cgtgccgaag cggtgcgcaa
     5641 ctggatgctg cagaccagcg acatcccggc cacctgtttt gccgtacagg gactgggcga
     5701 gagccagcct gcggcgacca acgacacgcc acagggccgg gcagtcaacc ggcgtgtcga
     5761 aatcagtctt gttccgcgtt ctgacgcctg tcaggacgtg aaataaaaca taccgccgga
     5821 agaaggcggt gcttcaatca cactaacaag gagagtaatt ctcatggcta ttcctgctta
     5881 tctctggctg aaagatgacg gcggcgcgga tatcaaaggt tccgtggacg ttcaggggcg
     5941 cgaaggtagc atcgaagtgg tggcgctgga tcacgatgtg tacatcccga ccgacaacaa
     6001 caccggcaaa ctgaccggta cccgtactca caagcccttt acgtttacca aagaaatcga
     6061 tgcctccagc ccgtatctct acaaagctgt gaccaccggc cagactctga aaacggcaga
     6121 atttaagttt taccgcatca acgatgccgg tcaggaagtg gagtacttca acatcacgct
     6181 tgataacgtc aagctggtca gagtcgctcc gcttatgcac gacatcaagg atccttccag
     6241 agagaagcat aaccacctgg aacgtattga gttccgctac gagaaaatca cctggactta
     6301 caaagacggc aacatcattc attccgactc gtggaatgag cgtccttccg cctgataact
     6361 gcggcagaca ggaaactgtc tgcctttttg ttgtgtgctt tgtccagtgc cgcaggcacc
     6421 ggtactgagc aaagcatacc cacaatctgg tgaaacctga tgggcctgag tgaggtaacg
     6481 gctatgggcg gtagcgtgcc tgaacgcgga tcgcaggaat acagaacacg ataaggaaac
     6541 cttctatgac cggaaatcac cccgccgcgc tgctgcgtcg ccttaaccca tactgtgcac
     6601 gggcgctgga cgctgccgcc tcactgtgtc agacccgcgc ccatgcggaa ataaccattg
     6661 aacactggct gctgaaactg ctggagcagg gagaaggcga tatcacggtg attgcccgcc
     6721 gctatgaatg ggatatcgac acgctctggc agtctctgct ggcacatctg gacaccttac
     6781 cccgcccggt ccgcgaacgt cctcaacttt ctgaaccgct ggcagcgctt atccgacagg
     6841 cgtggctgat agcgtcactg gaaggcgacg atccacaaat ccgcagccag catctgctga
     6901 tggcgctgac agaaaaaccg atgctgcccg cctgtaatga cctgtgggta ttgctgagcc
     6961 tgagccgtgt gcagcttgag cggctgcgtc ccctgctgga tgcgcagtcg gatgaatgtc
     7021 cggcacgtca gccacaggtc accgaaccgc tgacatccgc gcaacccggg gcggcaacga
     7081 cggacgcacc ggcaaaaacg ctgacgggga aacaggatga cgccctgctg gcggtgctta
     7141 accgctttac cgaagacgtg acggaaaaag cccgcagcgg gcgaatcgac ccggtattcg
     7201 ggcgcgacac ggaaattcgc cagatggtcg atatcctctc ccgtcgccgc aaaaacaacc
     7261 cgattctggt gggagaaccg ggggtgggca aaaccgcgct ggtggaaggg ctggcgctgc
     7321 gtatcaccga aggcaacgtg ccggacagcc tgaaaacggt gcatatccgc acactggacc
     7381 tcggtctgtt acaggctggc gcgggcgtta aaggtgaatt tgaacagcgg ctgaaaaatg
     7441 tcatcgatgc agtgcagaaa tcaccggagc cggtactgct gtttattgat gaagcccata
     7501 ccattatcgg tgcgggtaat caggcaggcg gcgcggatgc ggcgaacctg ctgaaaccgg
     7561 cgctggcaag gggcgaactg cgcaccatcg cggcgaccac gtggagcgaa tacaaacagt
     7621 attttgagcg cgacgccgcg ctggaacgcc gcttccagat ggtgaaagtc gacgagccgg
     7681 atgatgacac cgcctgcctg atgctgcggg gactcaaggc ccgctatgca cagcaccacg
     7741 gcgtacatat gctggacagc gccattcaga ccgccgtgcg cctgtcgcgc cgctatctga
     7801 ccggacgcca gttgccggac aaagcggttg atttgctgga taccgccggg gcaagagtcc
     7861 gcatgagcct tgacactctg ccggagccgt tgacgcagct tcatgcgcga ctggcggcgc
     7921 tggatatcga gcgggaggcg attgagcagg acagcgtgtt ttatcccgaa accagcccgg
     7981 agcggctggc ggaactgacc gatttgcgtg atgagctaca ggcagaagcc gggcatctgg
     8041 aagcgcagta tcagcaggaa aaggcactgg cgcagcagat tatgacgttg cgtcaggaag
     8101 gaacagacag ctctgaactg caacagcaac tgcggacgca tcagggcttt gcaccgctgc
     8161 tggcgctgga tgtggacgcc cgcgccgtcg ccacggtggt ggcggactgg accggcatcc
     8221 cgctctcttc cctgctcagg gacgagcaga gcgacctgct cagtatggaa cagagtcttg
     8281 aaaaccgcgt tgtcgggcaa agcccggcgc tctgcgccat cgcacagcgg ctgcgggcgg
     8341 ctaaaaccgg cctcacgccg gagaacggcc cacagggggt attcctgctg accggcccca
     8401 gcggcaccgg caaaaccgaa actgcgctca cactggccga caccctgttt ggtggtgaaa
     8461 aatcccttat caccattaat ctttcggaat accaggaacc gcataccgtt tcccagttga
     8521 aaggctcgcc tccaggctac gttggttacg gtcagggtgg cgtacttacc gaagccgttc
     8581 gcaaacgccc ttacagcgtg gtgctgctcg acgaagtgga aaaggcgcat cgcgacgtga
     8641 tgaacctgtt ctatcaggtg ttcgaccggg gctttatgcg cgacggcgaa gggcgggtaa
     8701 tcgacttccg caacaccgtc attctgatga ccgccaatct gggcagcgac cacatcatgc
     8761 aactgctgga ggaaaaaccg gacgccacgg acgcagacct gcatgaactg ctgtatcccc
     8821 ttctgcgaga ccattttcag cctgcactga tggcgcgttt tcagacggtg atttaccgcc
     8881 cgctgggaca ggaggcgatg cgcaccattg tggaaatgaa actggcgcag gtggtccgcc
     8941 gtcttcacca gcactacgga ctggaaacgg aaatcaacga cagcctgtat gaccagctta
     9001 ccgccgcctg cctgctgccg gataccgggg cgcgcaatat cgacagcctc ctgaaccagc
     9061 aaatcctgcc ggtcttaagc cagcagttgc tggcacagca ggccgtgcat cataagcctg
     9121 cccgactgcg gcttggctgg gatgaggagg acgggattgt actggaattt gatgagaaat
     9181 aatggaggct cttatgaacg tacagttctt cgaccacgca caccacaagc tcaaaattcg
     9241 cggcctgaaa agcccggtgg acgtgctgac ttttacaggc cacgaacagc tcagctcacc
     9301 cttccgctac gacattgagt ttaccagtac cgataaagcc atcgagccgg agtcggtact
     9361 gatgcaggac ggcgcattca gcctgagcgc cccgccggta caggggatgc cggtgcaggt
     9421 ccccctgcgc accctgcacg gcgtcattac cggctttaaa cacctctcct cgtcacagga
     9481 cgaagcccgt tacgaagtgc gccttgaacc ccgtatggcg ctgcttaccc gcagccgcca
     9541 gaacgccatc taccagaacc agacggtgcc gcaaatcgtc gaaaaaatcc tgcgcgagcg
     9601 gcaccagatg cgcggtcagg acttcgtgtt taacctcaaa agcg
//
LOCUS       DAPQZD010000178         9633 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPQZD010000178 DAPQZD010000000
VERSION     DAPQZD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.180"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            84..1061
                     /gene="csiD"
                     /locus_tag="RO454_004199"
     CDS             84..1061
                     /gene="csiD"
                     /locus_tag="RO454_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDY0935946.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1081..2346
                     /gene="lhgO"
                     /locus_tag="RO454_004200"
     CDS             1081..2346
                     /gene="lhgO"
                     /locus_tag="RO454_004200"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDY0935947.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            2372..3820
                     /gene="gabD"
                     /locus_tag="RO454_004201"
     CDS             2372..3820
                     /gene="gabD"
                     /locus_tag="RO454_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDY0935948.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3834..5114
                     /gene="gabT"
                     /locus_tag="RO454_004202"
     CDS             3834..5114
                     /gene="gabT"
                     /locus_tag="RO454_004202"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDY0935949.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5351..6751
                     /gene="gabP"
                     /locus_tag="RO454_004203"
     CDS             5351..6751
                     /gene="gabP"
                     /locus_tag="RO454_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDY0935950.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6772..7434
                     /gene="csiR"
                     /locus_tag="RO454_004204"
     CDS             6772..7434
                     /gene="csiR"
                     /locus_tag="RO454_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDY0935951.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7435..7884)
                     /gene="kbp"
                     /locus_tag="RO454_004205"
     CDS             complement(7435..7884)
                     /gene="kbp"
                     /locus_tag="RO454_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDY0935952.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7968..8126)
                     /locus_tag="RO454_004206"
     CDS             complement(7968..8126)
                     /locus_tag="RO454_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDY0935953.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8309..8608
                     /locus_tag="RO454_004207"
     CDS             8309..8608
                     /locus_tag="RO454_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0935954.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8618..9142
                     /gene="ygaP"
                     /locus_tag="RO454_004208"
     CDS             8618..9142
                     /gene="ygaP"
                     /locus_tag="RO454_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDY0935955.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9189..9593)
                     /gene="stpA"
                     /locus_tag="RO454_004209"
     CDS             complement(9189..9593)
                     /gene="stpA"
                     /locus_tag="RO454_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDY0935956.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2204 a         2498 c         2645 g         2286 t
ORIGIN      
        1 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
       61 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
      121 attcaggcca ggactatcgc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
      181 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
      241 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttca ggtaggcaaa attctcgacg
      301 atctgtgtgc gaatcagctg caaccgctgc tgttgaagac cctgttaaac cgcgctgaag
      361 gtgcgctgtt gatcaatgcg gtgggtatcg atgatgtcgc gcaggcggat gagatggtga
      421 agctggcgac ggcggtggcg catctgattg gccgttccaa tttcgatgcc atgagcggtc
      481 agtattacgc gcgtttcgtg gtgaaaaatg ttgataactc agacagctat cttcgtcagc
      541 cgcaccgcgt aatggagctg cacaacgacg gcacttacgt cgaagagatc actgattacg
      601 tgctgatgat gaaaatcgac gagcaaaaca tacagggcgg taactcgctg ctgctgcatc
      661 tcgatgactg ggaacatctg gacctctttt tccgccatcc gctggcgcgc cgtccgatgc
      721 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt cttccatccg gtgttcgatg
      781 tcgatcagca gggccgccca gtgatgcgct atatcgacca gtttgtccag ccgaaagact
      841 tcgaagaagg cgtgtggttg agcgagcttt cggacgccat tgaaaccagt aaaggcactc
      901 tttctgtacc cgttcccgtt ggcaaattcc tgttgattaa caacctgttc tggctgcacg
      961 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     1021 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     1081 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     1141 attgatatct atcctgacgc ccgcattgcg ttgctggaaa aagagtccgg ccctgcctgt
     1201 caccagacgg gccacaacag cggcgtgatc catgccggag tctattacac gcccggtagc
     1261 ctgaagacgc agttttgcct ggcggggaac cgcgccacta aagtcttttg cgatcaaaac
     1321 ggcattcgct acgacaactg cggcaagatg ctggtcgcca cgtctgaact cgaaatggaa
     1381 cggatgaatg cgttatggaa acgcacggcg gcaaacggga tcgagcgcga gtggttaaac
     1441 gccgaggagc tgcgcgagcg tgaaccgaat atcaccgggc ttggcggcat ttttgtgccg
     1501 tccagcggca ttgtcagcta ccgcgaagtg acggcggcga tggcaaaaat cttccaggcc
     1561 agaggtggtg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     1621 gtggtgatac gtacccgtca gggcggtgaa tatgaagcat cgacgctgat tagttgttcc
     1681 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     1741 ccgttccgcg gcgagtattt ccgccttgcg ccagagcata accagattgt taaccacctg
     1801 atttacccca ttcccgaccc ggcaatgccg tttttgggcg tgcatctcac ccgcatgatc
     1861 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctaccgc
     1921 aagcgcgact tctcatttag tgacacgctg gagattttag gctcgtcggg gattcgccgg
     1981 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     2041 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     2101 tggccccccg gtgtgcgggc gcaggcggta tcgccggacg gcaagctgat tgacgatttt
     2161 ctgtttgtca ccaccccgcg cacgatccac acctgtaatg cgccctcccc ggcagcgaca
     2221 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     2281 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     2341 aattaataac ctttgaaaac aggatgtagc gatgaaactt aacgacagta acttattccg
     2401 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggcgagg tcatcgacgt
     2461 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg ctgatgaaac
     2521 ccgcgccgct atcgacgccg ccagccgcgc cctgcccgcc tggcgtgcgc tcactgccaa
     2581 agaacgcgcc aacattctgc gcaactggtt caatttaatg atggagcatc aggacgattt
     2641 agcgcgcctg atgaccctcg aacagggcaa accactggct gaagcgaaag gcgaaatcag
     2701 ctacgccgcc tcatttattg agtggtttgc tgaagaaggc aaacgtattt atggcgacac
     2761 cattcccggt catcaggccg ataaacgcct gattgtgatc aagcagccga ttggcgtcac
     2821 cgcggctatc acgccatgga acttcccggc ggcgatgatt acccgtaaag ctggtccggc
     2881 gctggcggct ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     2941 ggcgctggca gaactggcga ttcgcgcggg cgttccggct ggcgtgttta acgtggtcac
     3001 cggttcggcg ggcgcggtcg gtaacgaact gaccagcaac ccgctggtgc gcaagctgtc
     3061 gtttaccggc tcgaccgaaa ttggccgtca gttaatggaa cagtgcgcga aagacatcaa
     3121 aaaagtgtcg ctggagctgg gcggcaacgc accgtttatt gtctttgacg atgccgatct
     3181 cgataaagcc gtggaaggcg cgctggcctc gaaattccgt aacgccgggc aaacctgcgt
     3241 ctgcgccaac cgcctgtatg tgcaggacag cgtgtatgac cgctttgccg aaaaattgca
     3301 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     3361 gttgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgctgatg cgctggagaa
     3421 aggcgcgcgc gtggtttgcg gcggtaaagc acatgaacgc ggcggtaact tcttccagcc
     3481 gaccattctg gtagacgttc cggctaacgc caaagtgtcg aaagaagaga cgtttggccc
     3541 cctcgccccg ctgttccgtt ttaaagatga agccgatgtg attgcgcaag ccaatgacac
     3601 cgaatttgga cttgcagcct atttctacgc ccgtgattta agccgcgtct tccgcgtggg
     3661 cgaagcactg gagtacggca tcatcggcat caacaccggc attatttcca atgaagtggc
     3721 cccgttcggc ggcattaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     3781 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaatgatca
     3841 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     3901 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     3961 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaagtgg
     4021 ttgcggcggt ggaagcgcaa ttgaaaaaac tgtcgcacac ctgcttccag gtgctggcct
     4081 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gattttgcca
     4141 agaaaactct gctggttacg accggttccg aagcggtgga aaatgcggtg aaaatcgccc
     4201 gcgccgccac taaacgtagc ggtactatcg cctttagcgg tgcgtatcac gggcgcacgc
     4261 attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     4321 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     4381 ctatcgccag catccaccgg atcttcaaaa atgacgccgc gccggaagat atcgccgcca
     4441 tcgtgattga gcccgttcag ggcgaaggcg gtttctacgc cgcgacgcca gcctttatgc
     4501 agcgtttacg cgcgctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     4561 gcggcgcagg gcgtaccggc acgctgtttg cgatggagca aatgggcgtg gcaccagatc
     4621 tcaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     4681 cggaagtgat ggacgccgtc gcaccgggcg gactgggtgg cacctatgcc ggtaacccga
     4741 ttgcctgcgt agcggcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     4801 aagccaacgt tctggggcag aagctgaaag acggattgct ggcgatagcc gaaaaacacc
     4861 cggagatcgg cgacgtacgc ggtctggggg cgatgatcgc catcgagctg tttgaagatg
     4921 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     4981 agggtctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccgc
     5041 tcaccattga agacgctcag atccgtcagg gtctggagat catcagccag tgttttgctg
     5101 aggcgaagct gtagcaccgc accatgccgg aggcgacgct ttgcggcttg tccggcctac
     5161 gagaatccag gtcggataag gcgtttacgc cgcatccgac aatctgtacg tgaacaggaa
     5221 taaatctatg tcggccggat aaggcggagc cgctctccgg caaaaagaac caataacaat
     5281 taaacgcgtg acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag
     5341 aggattcagg atggggcaat catcgcaacc acatgagtta ggcggcgggc tgaagtcacg
     5401 ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag
     5461 cgtcgccatc gccgaagcgg gcccggcggt attactggcc tatctgttcg ccggattact
     5521 ggtggttatg attatgcgga tgttggcgga aatggcggtt gccacgcccg ataccggttc
     5581 gttttccacc tatgccgata aagccattgg ccgctgggcg ggctatacca tcggctggtt
     5641 gtactggtgg ttttgggtac tggttatccc acttgaagcc aacatcgccg ccatgatcct
     5701 gcactcatgg gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt
     5761 aaccggcagt aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct
     5821 gtgtaaagtc atcgctatcc tggcctttat tttccttggt gccgtcgcaa ttagcggttt
     5881 ttacccgtat gccgacgtga gcgggatttc aagattgtgg gatagcggcg gctttatgcc
     5941 caacggcttc ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc
     6001 agaaattgtc accattgccg ccgcggaatc cgacacgccg gaaaaacata ttgttcgcgc
     6061 caccaactcg gttatctggc gtatctctat tttctatttg tgttctattt ttgtcgttgt
     6121 tgcattaatc ccgtggaata tgccagggct gaaagccgtt ggttcttatc gctcggttct
     6181 ggaattgctc aatattcctc atgcgaaatt aatcatggac tgcgtgatat tactttccgt
     6241 aaccagttgc ctgaactcgg cgctgtatac cgcttccagg atgctctact ccttaagccg
     6301 tcgcggtgat gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtacgtagc
     6361 ggtgttactc tccaccagcg cggcattctt aacggtggtg gtgaactatt acgcgcctgc
     6421 gaaggtgttt aaatttctga tcgacagctc cggcgctatc gccctgctgg tttatttagt
     6481 catcgccgtt tcacaattgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg
     6541 cctgcggatg tggctttatc cgtggctcac ctggctggtc atcggcttta ttacctttgt
     6601 gttggtagtg atgctattcc gcccggcgca acagttagaa gtgatctcca ccggcttatt
     6661 agcgattggg attatctgta ccgtgccaat tatggctcgc tggaaaaagc tgataatgtg
     6721 gcaaaaaaca cccattcata atacgcgctg ataatgatca ggagtcacac catgaccatt
     6781 acctctctgg atggctatcg ctggctgaag aacgatatta ttcgcggcaa ttttcagccg
     6841 gatgaaaaat tacggatgag tttgctgaca tcgcgttatg cacttggcgt tgggccgttg
     6901 cgggaagcac tttcgcaact ggtggcagaa cgactggtca cggtggtgaa tcaaaaaggg
     6961 tatcgggtgg cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat
     7021 atggaagcga tgctggtgag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac
     7081 gttctcgcaa aagcgcatct gctcagtaag cttgaggcct gtgacgccag tgagaaaatg
     7141 cttgatgagt gggatctgcg tcatcaggcg tttcatactg caattgtggc gggctgtggt
     7201 tcttactatt tgctgcaaat gcgtgaacgg ttgtttgatc tggcagcgcg ttatcgattt
     7261 atctggctgc ggcgaacagt gctttcggtg gaaatgctgg aagataaaca cgatcagcac
     7321 cagaccctga ctgcggcggt actggcgcga gataccgcgc gcgccagtga gttaatgcgc
     7381 cagcatttac tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc
     7441 ttcaggaata cgcaacactt gccccgggta aattttatcc gggcttttta gcatcggttt
     7501 attcgcttcg aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc
     7561 actcagagtg tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt
     7621 tttcacctga tcatcgacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt
     7681 ctccttcgcc tcctgactta agccgtcacc agtgaccgtc gctttgccgt cggcaatttg
     7741 aatgttcact ttatcggcat caggtatacc ggttttgttc agatgctcct gcaccttctt
     7801 cgcctgatcg tctttatcgt gctgacctgt aaccgcgtcc cagagttttt ctccggcatc
     7861 tttcacaaaa ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt
     7921 gtagcagagg aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt
     7981 tgcacccaga acgcgtgaat caaaccgggg atatagccaa gcagtgtcag cagaatatta
     8041 ataatgaacg cccaaccgaa ccctttaccg agcagcacgc cgagcggcgg cagaatgatg
     8101 gtgatgacga ttctccagaa acccatatgt actccctata agaaaattac tcattgttta
     8161 aaaagagatt ttatctctta agcgtagtaa ttttagcgga gcctgccagt ttcagcgtac
     8221 gcttttactc tcatttacgc tgctgatgct ggtatatgtt gcacacttag tttagagttt
     8281 acttaattta gaaaatactt aaacaactat gactgaactc gcacaattac aggccagtgc
     8341 cgaacaggca gcggccttat tgaaatcaat gagccaccct aaacgattgc tgattctgtg
     8401 tatgcttagc ggttcccccg gcaccagcgc gggagagctg acgcgcatta ccggactgag
     8461 tgcctctgcg acatcacagc atctcgctcg tatgcggggc gaagggctta tcgactgcca
     8521 acgggatgcc caacgcattc tatattccat taaaaatgag gcggtaaatg ccattatcgc
     8581 caccctgaaa aatgtctatt gtccgtaagg agtcactatg gctttgacaa ccatttcgcc
     8641 gcatgatgca caagaattaa tcgcacgcgg cgcaaagtta atcgatattc gtgatgctga
     8701 tgaatatctt cgtgaacata ttcctgaagc agatctggct ccattatccg tactggaaca
     8761 gtcaggtctt ccgcctaaat tacgtcgcga gcaaattata ttccactgcc aggcaggtaa
     8821 acgcaccagt aataacgctg ataaattagc agcgattgcc gcccccgcag aaatcttttt
     8881 actcgaagat gggattgatg gctggaaaaa agcaggattg ccagtagcgg taaataaatc
     8941 ccaaccctta ccgttaatgc gccaggtgca aatcgccgcg ggtggtttaa tattaatcgg
     9001 cgttgtactg ggttataccg taaatagcgt tttcttctta ttaagcggct ttgttggtgc
     9061 tgggttactg cttgcaggaa ttagtggttt ttgtggaatg gcaaggttgt tagataagat
     9121 gccgtggaac caacgagctt gagcagcgac gccggacgcg ccatagcagc gacatccggc
     9181 ctcagtaatt agatcaggaa atcgtcgaga gatttacctt ctgccagcgc ctgagcgatt
     9241 ggcttcggtg tacggccctg accggtccag gttttagttt caccgttaac atcgatgaat
     9301 ttatatttcg ccggacgcgg ctggcgtttt ttaccagcgc gtggtgccgc agcagagcta
     9361 ttacccaata actcttccgg gttaattccg tcagctttca tcagctccag ccaggtgcta
     9421 attttttcct ggcgctctgc cagttcacgc tgctgttgtt cttcttcttc acgtctttct
     9481 ttagtgacaa ccctgaattt ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga
     9541 gccatcgcac ggagggtgcg aatgttattt aaactttgta acattacgga cataataaag
     9601 aaaacctttt aacgccaaaa caaaaaagta ttt
//
LOCUS       DAPQZD010000179         9628 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPQZD010000179 DAPQZD010000000
VERSION     DAPQZD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.181"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            117..1055
                     /gene="tdcA"
                     /locus_tag="RO454_004210"
     CDS             117..1055
                     /gene="tdcA"
                     /locus_tag="RO454_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDY0935957.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            1154..2143
                     /gene="tdcB"
                     /locus_tag="RO454_004211"
     CDS             1154..2143
                     /gene="tdcB"
                     /locus_tag="RO454_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDY0935958.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2165..3496
                     /gene="tdcC"
                     /locus_tag="RO454_004212"
     CDS             2165..3496
                     /gene="tdcC"
                     /locus_tag="RO454_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDY0935959.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3522..4730
                     /gene="tdcD"
                     /locus_tag="RO454_004213"
     CDS             3522..4730
                     /gene="tdcD"
                     /locus_tag="RO454_004213"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDY0935960.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4764..7058
                     /gene="tdcE"
                     /locus_tag="RO454_004214"
     CDS             4764..7058
                     /gene="tdcE"
                     /locus_tag="RO454_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDY0935961.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7072..7461
                     /locus_tag="RO454_004215"
     CDS             7072..7461
                     /locus_tag="RO454_004215"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDY0935962.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7533..8897
                     /gene="tdcG"
                     /locus_tag="RO454_004216"
     CDS             7533..8897
                     /gene="tdcG"
                     /locus_tag="RO454_004216"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDY0935963.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9175..>9628
                     /locus_tag="RO454_004217"
     CDS             9175..>9628
                     /locus_tag="RO454_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935964.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGL"
BASE COUNT         2384 a         2208 c         2491 g         2545 t
ORIGIN      
        1 ggggtaacga tgctatggaa agtattgaaa gttaatttgt gagtggtcgc acatatcctg
       61 ttcatttcat tttgatacac ttcataccgt caatgaggta attaacgtag gtcgttatga
      121 gcactattct tcttccgaaa acgcagcacc tggtagtctt tcaggaagtt attagaagtg
      181 gttctatcgg ctcggctgca aaagagttag ggttaactca accggccgtc agtaaaatca
      241 ttaacgatat tgaagattat tttggggtgg agttagtggt gcggaaaaat accggtgtaa
      301 cattaacacc tgccggtcaa ttgttactct cccgttccga atccattacc cgtgaaatga
      361 aaaatatggt taatgagata agcggtatgt cttctgaggc ggtggtggaa gtctcatttg
      421 gttttccttc attgattggt tttactttta tgtcagggat gatcaacaag ttcaaagagg
      481 tgttcccgaa agcgcaggtt tctatgtatg aagcgcaact gtcttcgttc ttaccggcaa
      541 tccgcgacgg tcgactggat tttgcgattg gtacgttaag cgcagaaatg aagcttcagg
      601 atttacatgt tgagccgctg ttcgagtccg agtttgtgct ggtagccagt aagtcccgaa
      661 catgcaccgg caccgccacg ctggagtcgt tgaagaacga acagtgggtg ttgccacaaa
      721 ccaatatggg gtactacagc gaactactta ctacgttaca aagaaatggc atcagtattg
      781 aaaacatcgt taaaaccgac tcagtcgtga caatttataa tcttgttctc aatgctgatt
      841 tcttaactgt aattccttgt gatatgacat caccttttgg ttctaatcaa tttattacta
      901 ttccggttga agaaacatta cctgtggcac aatatgccgc ggtatggtcg aaaaattatc
      961 gtattaaaaa agcagcatcg gttttggtgg aattagccaa agagtattca tcttataatg
     1021 ggtgtagacg aaggcaatta attgaagttg attagttatt tgttttaatt taaacataaa
     1081 taatccacct gtctgttttg caggtgtcgg ttacggttac ctacatattt aattcaggct
     1141 aagaggtttt ataatgcata ttacatacga tctcccggtt gctattgatg acatcattga
     1201 agcaaaacaa cgactggctg ggcgaattta taaaacaggt atgcctcgct ccaactattt
     1261 tagtgaacgt tgcaaaggtg aaatattcct caaattcgaa aacatgcagc gtactggttc
     1321 atttaaaatt cgtggcgcat ttaataaatt aagttcactg actgatgcgg aaaaacgtaa
     1381 aggagtagtg gcctgctctg cgggcaacca tgcgcaaggg gtttcgctct cctgcgcgat
     1441 gctgggtatt gacggtaaag tggtgatgcc aaaaggtgcg ccgaaatcca aagtcgcggc
     1501 aacgtgcgac tactccgcag aagtcgtttt gcatggtgat aacttcaacg acactattgc
     1561 taaagtgagc gaaattgtcg aaatggaagg ccgtattttt atcccacctt acgatgatcc
     1621 gaaagtgatt gctggccagg gaacgattgg tctggaaatt atggaagatc tctatgatgt
     1681 cgataacgtg attgtgccaa ttggcggcgg cggtttaatt gctggtattg cggtggcaat
     1741 taaatctatc aacccgacca tccgtgttat tggcgtacag tctgaaaacg ttcacggcat
     1801 ggcggcttct ttccactccg gagaaataac tacgcaccga actaccggca ccctggcgga
     1861 tggttgtgat gtctcccgcc cgggtaattt aacttacgaa atcgttcgtg aattagtcga
     1921 tgacatcgtg ctggtcagcg aagacgaaat cagaaacagt atgattgcct taattcagcg
     1981 caataaagtc gtcaccgaag gcgcaggcgc tctggcatgt gctgcattat taagcggtaa
     2041 attagaccaa tatattcaaa acagaaaaac cgtcagtatt atttccggcg gcaatatcga
     2101 tctttctcgc gtctctcaaa tcaccggttt cgttgacgct taattaattc gttgaggata
     2161 ggatatgagt acttcagata gcattgtatc cagccagaca aaacaatcgt cctggcgtaa
     2221 atcagatacc acatggacgt taggcttgtt tggtacggca atcggcgccg gggtgctgtt
     2281 cttccctatc cgcgcaggtt ttggcggact gatcccgatt cttctgatgt tggtattagc
     2341 ataccccatc gcgttttatt gccaccgggc actggcgcgt ctgtgtcttt ctggctctaa
     2401 cccttccggc aacattacgg aaacggtgga agagcatttt ggtaaaactg gcggcgtggt
     2461 tatcacgttc ctgtacttct tcgcgatttg cccactgttg tggatttatg gcgttaccat
     2521 taccaacacc tttatgacgt tctgggaaaa ccagctcggc tttgcaccgc tgaatcgcgg
     2581 ctttgtggcg ctgttcctgt tgctgctgat ggctttcgtc atctggtttg gtaaggatct
     2641 gatggttaaa gtgatgagct atctggtatg gccgtttatc gccagcctgg tgctgatttc
     2701 tctttcgctg atcccttact ggaactctgc agttatcgac caggttgacc tcggttcgct
     2761 gtcgttaact ggtcatgacg gtatcctgat caccgtctgg ctggggattt ccatcatggt
     2821 tttctccttt aacttctcgc caatcgtctc ttccttcgtg gtttctaaac gtgaagagta
     2881 cgagaaagat tttggtcgcg acttcaccga gcgtaaatgt tcacaaatca tctctcgcgc
     2941 cagtatgctg atggttgcag tggtgatgtt ctttgccttt agctgcctgt ttacgctgtc
     3001 tccggcgaat atggctgaag cgaaagcgca gaatattcca gtgctttctt atctggctaa
     3061 ccactttgcg tccatgaccg gtaccaaaac aacgtttgcg attacactgg aatatgccgc
     3121 ttccatcatc gcactcgtgg ctattttcaa atctttcttc ggtcactatc tggggacgct
     3181 ggaagggctg aatggtttga ttctgaagtt cggttataaa ggtgacaaaa ccaaagtgtc
     3241 gctgggtaaa ctgaatacta tcagcatgat tttcattatg ggctccacct gggttgttgc
     3301 ctacgccaac ccgaacatcc tcgacttgat tgaagccatg ggcgcaccga ttatcgcatc
     3361 actgctgtgc ctgttgccga tgtatgccat ccgtaaagcg ccgtctctgg cgaaataccg
     3421 tggtcgtctg gataacgtgt ttgttaccgt gattggtctg ctgaccatcc tgaacatcgt
     3481 atacaaactg ttttaatccg taactcagga tgagaaaaga gatgaatgaa tttccggttg
     3541 ttttggttat taactgtggt tcgtcttcga ttaagttttc cgtactcaat gccagcgact
     3601 gtgaagtatt aatgtcaggt attgccgacg gtattaactc ggaaaatgca ttcttatccg
     3661 taaatggggg agagccagca ccgctggctc accacagcta cgaaggtgca ttgaaggcaa
     3721 ttgcatttga actggaaaaa cggaatttaa atgacagtgt ggctttaatt ggccaccgca
     3781 tcgctcacgg cggcagtatt tttaccgagt ccgccattat taccgatgaa gtcattgata
     3841 atatccgtcg cgtttctcca ctggcacccc tgcataatta cgccaattta agtggtattg
     3901 aatcggcgca gcaattattc ccgggcgtaa ctcaggtggc ggtatttgat accagtttcc
     3961 accagacgat ggctccggaa gcttatttat acggcctgcc gtggaaatat tatgaagagt
     4021 taggtgtacg ccgttatggt ttccacggca cgtcgcaccg ctatgtttcc cagcgcgcac
     4081 attcgctgct gaatctggcg gaagatgact ccggcctggt tgtggcgcat cttggcaatg
     4141 gcgcatcaat ctgcgcggtt cgcaacggtc agagtgttga cacttcaatg ggaatgacgc
     4201 cgctggaagg cttgatgatg ggtacccgca gtggtgatgt cgacttcggg gcgatgtcct
     4261 gggtcgccag ccaaaccaac cagagcctgg gggacctgga acgcgtagtg aataaagagt
     4321 cgggattatt aggtatttcc ggcctttctt cggatttacg tgttctggaa aaagcctggc
     4381 atgaaggtca cgaacgcgcg caactggcaa ttaaaacctt tgttcaccga attgcccgtc
     4441 atattgccgg acacgctgct tcattacatc gcctggatgg aattatattc accggcggaa
     4501 taggagagaa ttcaagttta attcgtcgtc tggtcatgga acatttagct gtattaggcg
     4561 tagaaattga tacagaaatg aataatcgct ctaactcttt tggagagcga attgtttcca
     4621 gtgaaaatgc gcgtgtcatt tgcgccgtaa ttccgactaa cgaagaaaaa atgattgctt
     4681 tggatgccat tcatttaggc aaagtgaacg cgcccgcaga atttgcataa tttagttgaa
     4741 gtattgtaga gagattattt ttcatgaagg tagatattga taccagcgat aagctgtacg
     4801 ccgacgcatg gcttggcttt aaaggtacgg actggaaaaa cgaaattaat gtccgcgatt
     4861 ttattcaaca taactataca ccttatgaag gcgatgaatc ttttctcgcc gaagcgacgc
     4921 ctgccaccac ggaattgtgg gaaaaagtca tggaaggcat ccgtatcgaa aatgcgactc
     4981 acgcgccatt ggatttcgat accaatattg ccaccacaat taccgctcat gacgcgggat
     5041 atattaacca gccgctggaa aaaatagtgg gtctgcaaac ggatgcgcca ttaaagcgtg
     5101 cgctgcatcc gttcggcggc attaatatga ttagaagttc attccacgcg tatggtcgtg
     5161 aaatggatag cgaatttgaa tacatcttta ccgatctgcg taaaacccat aatcagggcg
     5221 tatttgatac ttactcaccg gatatgctgc gctgccgtaa atctggtgta ctgaccggtt
     5281 taccagatgg ttatggtcgt gggcgtatca tcggtgacta tcgccgtgtg gcactgtacg
     5341 gcatcagtta tctggtacgt gaacgcgaac tgcagtttgc cgatctccag tcccgtctgg
     5401 aaaaaggcga agatctcgaa gccaccattc gtctgcgtga ggaactggca gaacatcgcc
     5461 gtgcgctgtt acagattcag gaaatggcgg cgaaatatgg ctttgatatc tctcgtccag
     5521 cgcagaatgc acaagaagcc gtgcagtggc tctacttcgc ttacctggcg gcagtgaaat
     5581 cgcaaaatgg cggcgcgatg tcgctggggc gcacggcatc gttcctcgat atctacattg
     5641 agcgcgactt taaagcaggc gtactcaatg agcagcaggc acaggaactg atcgaccact
     5701 tcatcatgaa gatccgtatg gtgcgcttct tgcgtacgcc ggaatttgat tcgctgttct
     5761 ccggcgaccc gatctgggcg acggaagtga tcggcgggat ggggttggac ggtcgaaccc
     5821 tggtgaccaa aaactccttc cgctatttac acaccctgca cactatgggg cctgcaccgg
     5881 aacctaacct gactattctg tggtcggaag ccttgccgat tgccttcaaa aaatatgccg
     5941 cgcaggtgtc tatcgtcaca tcctctttgc agtatgaaaa cgacgatctg atgcgtactg
     6001 acttcaatag cgacgattac gcgattgcct gttgcgtcag cccaatggtg attggtaagc
     6061 agatgcagtt cttcggtgcg cgcgctaacc tggcgaaaac gctactgtac gcaatcaacg
     6121 gcggaatgga cgagaagctg aagattcagg ttggcccgaa aacggctccg ttgatggatg
     6181 acgtgctgga ctacgacaaa gtgatggata gcctcgacca cttcatggac tggctggcgg
     6241 tacagtacat cagcgcgctg aatatcattc actacatgca cgacaagtac agctacgaag
     6301 cttcgctgat ggcgctacat gatcgtgatg tctatcgcac catggcgtgc ggcatcgcgg
     6361 gcctgtcggt ggcaacggac tccctgtctg ccatcaaata tgctcgtgtg aaaccaatcc
     6421 gtgatgaaaa cggcctggcg gtggatttcg agattgacgg cgaatatccg cagtatggca
     6481 acaacgacga gcgcgtagac agcattgcct gcgatctggt tgaacgcttt atgaagaaaa
     6541 ttaaagcttt gccaacctat cgcaatgctg tgccgaccca atcgattctg actatcacct
     6601 ctaacgtggt gtacggtcag aaaaccggga acacgccgga cggtcgccgc gccggaacac
     6661 cgttcgcgcc gggcgctaac ccgatgcacg gtcgtgatcg caaaggtgcc gtggcctcat
     6721 tgacgtcggt ggcgaaactg ccgttcacct acgccaaaga tgggatctcg tacaccttct
     6781 ccatcgtccc tgcggcgctg ggcaaagaag atccagtacg taaaaccaac cttgtcggcc
     6841 tgctggatgg gtatttccac cacgaagcgg atgtcgaagg cggtcaacac ctcaacgtca
     6901 acgtaatgaa tcgggaaatg ctgctggatg ccatcgagca cccggaaaaa tatcctaacc
     6961 tgacaatccg tgtctctggc tacgccgtgc gcttcaacgc actgacccgt gaacagcaac
     7021 aggatgttat ttcacgtacc tttacccagg cgctctgacg ccggaggatg tatgaaaaag
     7081 attatcgaaa cgcaacgtgc cccaggcgca atcggccctt atgttcaggg cgttgattta
     7141 ggcagcatgg tcttcacctc cgggcaaatt ccggtttgcc cacagaccgg tgagatcccg
     7201 gctgatgtgc aagatcaggc gcgtttaagc ctcgaaaacg tcaaagcgat cgtggttgct
     7261 gccgggctga gtgtgggcga tatcatcaag atgaccgtgt tcatcaccga tctgaatgat
     7321 tttgccacca tcaatgaagt ctataagcag ttcttcgatg aacatcaggc gacctacccg
     7381 acccggagct gtgtgcaggt cgcgcgtttg ccgaaagatg tgaagctgga aattgaagcc
     7441 atcgcagtac gtagtgcgta aagcctcgtg agcgggacgg tcgcaaggtc gttccgctcc
     7501 acttcactga acggcaatcc gagggtgtgg atatgattag tgcattcgat attttcaaaa
     7561 ttgggattgg tccctccagt tcgcatactg tggggccaat gaatgccgga aaaagtttta
     7621 ttgatcggct ggaaagtagc ggcttattaa ccgcgacgag ccatattgtg gtcgatctgt
     7681 acgggtcgtt gtcactgacg ggcaaaggcc atgccacgga tgtcgccatc atcatgggac
     7741 tggcaggaaa cagtccgcag gatgttgtca ttgatgagat ccctgcattt atagagttag
     7801 tgacgcgcag cgggcggttg ccggtggctt ctggcgcgca tattgttgat tttcctgtag
     7861 caaagaacat tatcttccat cccgaaatgt tgcctcgcca tgagaacgga atgcggatca
     7921 ctgcctggaa gggacaggaa gagctattaa gcaaaaccta ttactctgtc ggcggcgggt
     7981 ttattgtcga agaagaacac ttcggcctgt cgcacgatgt cgaaacaccc gtaccttacg
     8041 atttccactc agcaggtgaa ctgctgaaaa tgtgtgatta caacggcctg tctatatctg
     8101 gcctgatgat gcacaacgag ctggcgctgc gcagtaaagc ggaaattgac gcagggtttg
     8161 cccgtatctg gcaagtgatg catgacggta ttgaacgtgg aatgaatact gaaggcgtgc
     8221 tgcctggtcc gctcaatgtg ccgcgccgtg ccgtagcgct gcgtcgtcaa ctggtttcca
     8281 gcgataacat ctctaacgat ccgatgaatg tcatcgactg gatcaacatg tacgcgctgg
     8341 cggttagtga agaaaacgca gctggcgggc gcgtggtaac ggcaccgact aacggtgcgt
     8401 gcggcattat tccggcagtg ctggcttatt acgataagtt ccgtcgtccg gtaaacgagc
     8461 ggtcaattgc ccgctatttt ctggcagcgg gggcaattgg cgcgctgtat aaaatgaacg
     8521 cctccatctc tggcgcggaa gtcggctgtc agggggagat tggcgtggcc tgttcaatgg
     8581 cagcggcagg gttaactgaa ctactgggtg gcagtccggc gcaggtatgc aatgcggcgg
     8641 aaatcgcgat ggagcataac cttgggctga cctgcgatcc ggttgccgga caggtacaaa
     8701 tcccgtgcat tgaacgtaat gccattaatg ccgtgaaagc agtaaacgcc gcgcggatgg
     8761 cgatgcgccg cacctcggca ccgcgtgttt cactcgataa agtgatcgag acgatgtatg
     8821 aaaccggcaa agatatgaac gataaatacc gcgaaacatc acgcggagga ctggctatta
     8881 aagtggtctg cggctgacat tgctgtcagc tatcatcctt gggtgcacaa aatgtgcacc
     8941 cttttttatt gtgatctgcc gcaaatagag aataattatt tctattaact caatttaata
     9001 gaaaattatt acccacaatt gctatttaaa agtcttgatt gtgaagattt tctctgatct
     9061 cacgcgtatc atttctttat tctaataact cgcctgcaaa aacgtagacc gcatatcgcc
     9121 ttgatgcggc tcggaaaaaa ataataagcc ctacaaaata ttgagcgaga aattatggaa
     9181 attgcatcga ataaaggcgt cattgcagac gcttcgaccc cggcgggtcg tgctggaatg
     9241 agtgagagcg agtggcgaga agcgatcaaa ttcgacagta ctgacactgg ctgggtgatt
     9301 atgagtatcg ggatggcgat tggcgcgggg attgtttttc tcccggtgca ggtcggtttg
     9361 atgggattgt gggtattttt gctctcatcg gtgattggtt acccggcaat gtatctgttt
     9421 cagcggttgt ttattaatac gctggcagaa tcaccagaat gtaaagatta cccgagcgtc
     9481 atcagcggtt atttaggtaa aaactggggc atcctgttag gtgcactcta tttcgtaatg
     9541 ctggtgattt ggatgttcgt ctattccacc gccatcacca acgatagtgc ttcctacctg
     9601 cataccttcg gcgtgacgga agggttgc
//
LOCUS       DAPQZD010000180         9593 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPQZD010000180 DAPQZD010000000
VERSION     DAPQZD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.182"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            195..245
                     /gene="hisL"
                     /locus_tag="RO454_004218"
     CDS             195..245
                     /gene="hisL"
                     /locus_tag="RO454_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDY0935965.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    221..344
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            391..1290
                     /gene="hisG"
                     /locus_tag="RO454_004219"
     CDS             391..1290
                     /gene="hisG"
                     /locus_tag="RO454_004219"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDY0935966.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1296..2600
                     /gene="hisD"
                     /locus_tag="RO454_004220"
     CDS             1296..2600
                     /gene="hisD"
                     /locus_tag="RO454_004220"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDY0935967.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2597..3667
                     /gene="hisC"
                     /locus_tag="RO454_004221"
     CDS             2597..3667
                     /gene="hisC"
                     /locus_tag="RO454_004221"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDY0935968.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3667..4734
                     /gene="hisB"
                     /locus_tag="RO454_004222"
     CDS             3667..4734
                     /gene="hisB"
                     /locus_tag="RO454_004222"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDY0935969.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGAQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4734..5324
                     /gene="hisH"
                     /locus_tag="RO454_004223"
     CDS             4734..5324
                     /gene="hisH"
                     /locus_tag="RO454_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDY0935970.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIEED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGTAGAKLLKNFLEM"
     gene            5324..6061
                     /gene="hisA"
                     /locus_tag="RO454_004224"
     CDS             5324..6061
                     /gene="hisA"
                     /locus_tag="RO454_004224"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDY0935971.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVTGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVFLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6043..6819
                     /gene="hisF"
                     /locus_tag="RO454_004225"
     CDS             6043..6819
                     /gene="hisF"
                     /locus_tag="RO454_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDY0935972.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6813..7424
                     /locus_tag="RO454_004226"
     CDS             6813..7424
                     /locus_tag="RO454_004226"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDY0935973.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNILNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLATVIENLRKRHQ"
     gene            complement(7610..8587)
                     /gene="wzzB"
                     /locus_tag="RO454_004227"
     CDS             complement(7610..8587)
                     /gene="wzzB"
                     /locus_tag="RO454_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDY0935974.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQATPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGVGIVLGRNALRNYNAK"
     gene            complement(8733..>9593)
                     /locus_tag="RO454_004228"
     CDS             complement(8733..>9593)
                     /locus_tag="RO454_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDY0935975.1"
                     /translation="DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREG
                     KALYDNLHPSRIVIGERSERAERFAALLQEGTIKQNIPTLFTDSTEAEAIKLFANTYL
                     AMRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLL
                     ANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQG
                     IMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADK
                     VYTRDLFGSD"
BASE COUNT         2348 a         2513 c         2539 g         2193 t
ORIGIN      
        1 gcgagacgaa aaatcagact ttgctctttt tggtgctgtc aagttagagg acagtcctct
       61 tagccccctt ctttccccgc tcattcatta aacaaatcta tcgccataaa atatataaaa
      121 aagcccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat
      181 tcacagagac ttttatgaca cgcgttcaat ttaaacacca ccatcatcac catcatcctg
      241 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg cgcatgaacg
      301 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcacg attgataacg
      361 gttcagacag gtttaaagag gaataagaaa atgacagaca acactcgttt acgcatagct
      421 atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tactggcgcg ctgtggcatt
      481 aaaatcaatc ttcacaccca gcgtctgatc gcgatggcag aaaacatgcc gattgatatt
      541 ctgcgcgtgc gtgacgacga tattcccggt ctggtaatgg acggcgtggt agaccttggg
      601 attattggcg aaaacgtgct ggaagaagag ctgcttaacc gccgcgccca gggtgaagat
      661 ccgcgctact ttaccctgcg tcgtctggat ttcggcggct gccgcctttc gctggcaacg
      721 ccggttgatg aagcctggga cggtccgctc tccttaaacg gtaaacgtat cgccacctct
      781 tatcctcacc tgctcaagcg ttatctcgac cagaaaggta tctcttttaa atcctgctta
      841 ctgaacggtt ctgtcgaagt tgcgccgcgt gccgggctgg cggatgcgat ttgcgatctg
      901 gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt tatctaccgc
      961 tcgaaagcct gcctgatcca gcgcgatggc gaaatggaag aatccaaaca gcaactgatc
     1021 gacaaactgc tgacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg
     1081 atgcacgcac cgaccgaacg cctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
     1141 ccaaccattc tgccgctggc gggtgaccaa cagcgcgtag cgatgcacat ggtgagtagc
     1201 gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcgc tgggtgccag ttcaatcctg
     1261 gtcctgccga ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact
     1321 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
     1381 ctgaaagcat tacccgcacc gttaacgata ttctcgataa cgtgaaaacg cgtggcgatg
     1441 aggccctgcg tgaatacagc gcgaagtttg ataaaaccac ggttaccgcg ctgaaggtgt
     1501 ctgctgatga gatcgccgcc gccagcgaac gcctgagcga cgagctaaaa caggcgatgg
     1561 cggtggcagt aaagaatatt gaaaccttcc acactgcgca aaaactgccg ccggtagatg
     1621 tagaaacgca gccaggcgtg cgttgccagc aggtcacgcg cccggtggct tcagtgggtt
     1681 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcgactccgg
     1741 cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga
     1801 tcctttatgc ggcgcaactg tgcggtgtgc aggacgtgtt taacgtcggc ggcgcacagg
     1861 ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc
     1921 cgggtaacgc ctttgtcacc gaagcaaaac gccaggtaag ccagcgtctg gacggtgcgg
     1981 cgatcgatat gcccgcaggc ccgtcggaag tgctggtgat tgctgacagc ggcgcaacgc
     2041 cggatttcgt ggcttctgat ttgctctccc aggctgaaca cggcccggac tcacaggtga
     2101 ttttactgac acctgatgct gatatggcgc gtcgcgttgc cgaggccgtc gaacgccagt
     2161 tggcagaatt gccgagagct gaaaccgccc gtcaggcact gagcgccagc cgcctgatcg
     2221 taaccaacga tttagcgcag tgcgtagaga tctccaatca gtacggtccg gagcacctga
     2281 tcattcagac ccgcaacgcc cgcgatctcg tcgatagcat taccagcgcc ggttcggtat
     2341 ttctcggtga ctggtcaccg gaatcggcag gtgattacgc ctccggcacc aaccacgtgc
     2401 tgccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaaac
     2461 gcatgaccgt acaggaactg tcgaaagaag gtttctccgc gctggcttcg accattgaaa
     2521 cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc cgttactttg cgtgttaacg
     2581 tccttaagga gcaagcatga gcaccgtgac tattactgat ttagcgcgtg aaaacgtccg
     2641 caacctgacg ccgtatcagt cggcgcgtcg tctgggcggt aacggcgacg tctggctgaa
     2701 cgccaacgaa tatcccacag ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
     2761 cccggaatgc cagccaaaag cggtgattga aaattacgcg caatatgcag gcgtaaaacc
     2821 ggaacaggtg ctggtcagcc gtggcgcgga cgaaggtatt gaactgctga tccgcgcttt
     2881 ttgcgaaccg ggtaaagacg ccatcctcta ctgcccgcca acgtacggca tgtacagcgt
     2941 cagcgctgaa accattggcg tcgaatgccg cacagtgccg acgctggaaa actggcaact
     3001 ggacttacag ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc
     3061 caacaacccc accgggcaac tgatcaaccc acaggatttt cgcaccctgc tggagttaac
     3121 ccgcggtaag gcgattgtgg ttgccgatga agcctatatc gagttttgcc cgcaggcatc
     3181 gctggctggc tggctggcgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc
     3241 ttttgctctg gcggggcttc gttgcggatt tacgctggca aacgaagaag tcatcaacct
     3301 gctgatgaaa gtgatcgccc cctacccgct ctcgacgcca gttgccgaca ttgccgcaca
     3361 ggcgttaagc ccgcagggaa tcgtcgccat gcgcgaacgg gtggcgcaaa ttattgcaga
     3421 acgcgaatac ctgattgccg cactgaaaga gatcccctgc gtggagcagg ttttcgactc
     3481 tgaaaccaac tacattctgg cgcgctttaa agcctccagt gcagtgttta aatctttgtg
     3541 ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
     3601 aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
     3661 agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
     3721 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaacctgg cgtgatcccg
     3781 gagctgctga agctgcaaaa agcgggctat aagctggtga tgatcaccaa tcaggatggt
     3841 ctgggagcac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag
     3901 atcttcacct cgcaaggcgt gcagtttgat gaagtgctga tttgcccaca cctgcccgcc
     3961 gatgagtgcg actgccgtaa gccgaaagta aaactggtgg agcgttatct ggctgagcaa
     4021 gcgatggatc gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg
     4081 gaaaacatgg gcattaatgg tttacgctac gaccgcgaag ccctgaactg gccaatgatt
     4141 ggcgagcaac tcactaaacg agaccgttac gcccatgtag tgcgcaacac caaagagacg
     4201 caaattgacg tccaggtgtg gctggatcgc gagggtggca gcaagattaa caccggcgtt
     4261 ggcttctttg atcacatgct ggatcagatc gccacccacg gcggtttccg tatggaaatc
     4321 aacgttaaag gcgacctcta tatcgacgat catcacaccg tcgaagatac cggcctggcg
     4381 ctgggggaag cattaaaaat tgctcttggc gacaaacgcg gtatttgccg gtttggtttt
     4441 gtgctgccga tggacgaatg ccttgcccgc tgcgcactgg atatctctgg tcgcccgcac
     4501 ctggaatata aagccgagtt tacctaccag cgtgtgggcg atctcagcac cgagatgatc
     4561 gagcacttct tccgttcgct ctcatacacc atgggcgtga cgctacacct gaaaaccaaa
     4621 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cgtttggccg caccctgcgc
     4681 caggccatcc gcgtggaagg cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg
     4741 tggtgatcct tgatactggc tgcgccaacc tgaactcggt gaagtctgcc attgcgcgtc
     4801 acggttatga acccaaagtc agccgtgacc cggacgtcgt gttgctggcc gataaactgt
     4861 ttttacccgg cgttggcacc gcacaagcgg cgatggacca ggtgcgcgag cgcgagctgt
     4921 ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg atgcaactgc
     4981 tggggcggcg cagcgaagag agcaacggcg tcgacttgct gggcatcatc gaagaagacg
     5041 tgccgaaaat gaccgacttt ggtctgccac tgccacatat gggctggaac cgcgtttacc
     5101 cgcaggcagg caaccgcctg tttcagggga ttgaagacgg cgcgtacttt tactttgttc
     5161 acagctacgc aatgccagtc aatccgtgga ccatcgccca gtgtaattac ggcgaaccgt
     5221 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
     5281 gtaccgctgg cgctaagttg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
     5341 agatttaatc gacggcaccg tggtgcgtct ccatcagggc gattatggca aacagcgcga
     5401 ttacggtaac gacccgctgc cgcgcttaca ggattacgcc gcacaaggtg ccgaagtact
     5461 gcacctggtg gatctgaccg gggcaaaaga tccggctaaa cgtcaaatcc cgctgattaa
     5521 aaccctggtc acgggcgtta acgtcccggt gcaggttggc ggcggcgtgc gtagcgaaga
     5581 agacgtggcg gcgttactgg aagcaggcgt tgcgcgtgtg gtggttggct ccaccgcggt
     5641 gaaatcacca gaaatggtga agggctggtt tgaacgtttt ggtgccgatg ccttagtgct
     5701 ggcgctggat gtccgtatcg acgagcaagg caacaagcag gtggcagtca gcggctggca
     5761 agagaactcg ggcgtttcac tggaacaact ggtggaaacc tatctgcccg tcggcctgaa
     5821 acatgtgctg tgtaccgata tctcgcgcga cggcacgctg gcgggatcta acgtcttttt
     5881 atatgaagaa gtgtgtgcca gatatccgca agtggcgttt cagtcctccg gcggcattgg
     5941 cgatattaac gatgtggcgg ccctgcgtgg tactggtgtg cgcggcgtaa tagttggtcg
     6001 tgcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata
     6061 atcccgtgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
     6121 gaaatcattg gcgatatcgt gccactggca aaacgctacg ccgaagaagg tgcagatgaa
     6181 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
     6241 tctcgcgtgg cggaagtaat cgacattccg ttttgtgtgg cgggtgggat taagtctctg
     6301 gaagatgccg cgaaaattct ttcctttggc gcagataaaa tctccatcaa ctctcctgcg
     6361 ctggcggacc cgacgttaat tactcgcctg gccgatcgct ttggcgtgca gtgtattgtg
     6421 gtcggtattg atacctggta cgacgccgaa accggtaaat atcatgtgaa tcaatatacc
     6481 ggcgatgaaa gccgcacccg cgttactcag tgggaaacgc tcgactgggt acaggaagtg
     6541 caaaaacgcg gtgccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac
     6601 ggttacgacc tcgagcagct gaaaaaagtg cgtgaagttt gccacgtccc gctgattgcc
     6661 tccggtggcg cgggcaccat ggaacacttt ctcgaagcct tccgcgatgc cgacgttgac
     6721 ggcgcgctgg cagcttccgt attccacaaa caaataatca atattggtga attaaaagcg
     6781 tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa cgtcgcgaac
     6841 tggactggga aaaaaccgac ggtctgatgc cggtgattgt gcaacacgcg gtatccggcg
     6901 aagtgctaat gctgggctat atgaacccgg aagccttaga caaaaccctc gaaagcggca
     6961 aagtcacctt cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca
     7021 atattctcaa tgtggtgagt attgccccgg actgcgacaa cgacacgtta ctggtgctgg
     7081 caaatcccat cggcccgacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc
     7141 accagtggct gttcctttat caactggaac aactgctcgc cgaacgtaaa tctgccgacc
     7201 cggaaacctc ctacaccgcc aaactgtatg ccagcggcac taaacgcatt gcgcagaaag
     7261 tgggcgaaga aggcgtggaa accgcactgg cagcaacggt acatgaccgc tttgagctga
     7321 ccaacgaggc atctgacttg atgtatcacc tgctggtgtt gttgcaggat caggatctgg
     7381 atttagcgac ggtaattgag aacctgcgta aacggcatca gtgagttgcg tgctgaccgg
     7441 atgcgatgtt gttgtcgcat ccggagccat tgcccgatgc gcaagcttat cgtgcctaca
     7501 cattatctca acacacgtag gccggataag atgcgccagc atcgcatccg gcaaaaaaaa
     7561 cgggcatggt gtcaccaccc tgcccgtttc tcttaaatgc acaataatat tatttcgcgt
     7621 tgtaattacg cagcgcgtta cgccccaaca caatccccac gccaaccatg ccgcccagca
     7681 gcaccgccag aatcaaggta attgcctttt tcgggctatc gcgacgaata ggtaacgtcg
     7741 gtttcattac atagcggtaa gcatgaatat caagtttatc aacgtcaaga ttgtcgatat
     7801 ccagcaggtt ttgacgagtc tgatagtagc ttgatgagaa caccaacgga cgggtcgcct
     7861 cgtgcttaat catcgactcc agcgcttcgc tccccaaaag gaacatagta tcctgggtta
     7921 cgtcctgcgt ctgttgaatc tgcggctttg tcacctgcgc ctgattcgca tactgcaacg
     7981 cttcctgaat ctgacggata cgcagatctt tctgttcctg cgcgaccact tcctgggttc
     8041 ttaaagagtc ctgcaagttt ttccgtccca gagcaatgtt gtccttgaga tccttttcta
     8101 gctcttgatt cactttatca tcaacttgct gaatgtattg ggccaacttc atttgtgcgc
     8161 cctctgcagt ttgcccaaca taagaaacag tcaatggtaa ttgctggttc ttaacagaag
     8221 gttcgatggt aagtttttct ggctcttcct gattatccag cgtttctgct aatgcagaga
     8281 aggcagaact gaagcgacca attaacgtct cctgcaaatc cgatactttc ggtgtagcct
     8341 gaccatagat aacattcatg gcattgttat agccagcaat ttgccccacg tcgggctgag
     8401 tgataattgc tgttgacgtc catttctcct tcgctactgc caaataacca atagccaggg
     8461 caatagccac aatgacggaa atgatgattg tcatcttgcc acgccacaac tgcactagta
     8521 aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta ttattttcta
     8581 ctctcatcat taccctaact gacggaaatt tccaagtccg catagtttag cgataatcct
     8641 cgtgaatgct ataggataag tgataaaaat aatgaattgt tgaggaagta acaaaagaca
     8701 atcagggcgt aaatagccct gataacaaga tgttaatcgc taccaaagag atcgcgggtg
     8761 tataccttat ctgccacatc cttaagctct tctgccatac gattggagat aatgacatca
     8821 gcttgttgtt tgaaagtggc gagatctcgt tccaggcgag agttgaagaa tgagtcttct
     8881 ttcatcaccg gttcgtaaat gatcacttca acgcctttcg ccttgatacg tttcataatc
     8941 ccctgaatgg aagacgcgcg gaagttgtct gaaccgctct tcataatcag acgataaata
     9001 cccaccactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt acgcgtgcgg
     9061 ttagcatcga caattgccga aatcaggtta ttcggcacag actgataatt cgccaataac
     9121 tgtttggtat ctttcggcaa acaataacca ccataaccaa acgacggatt gttgtaatgg
     9181 ttgccgatac gcgggtcgag acaaacacct tcgataatct gacgcgtatt cagacccaaa
     9241 ctttctgcat agctatccag ttcgttaaag tacgccacgc gcatcgccag ataggtatta
     9301 gcgaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtcgg gatattttgc
     9361 ttaatcgtgc cttcctgtaa caacgcagca aaacgttctg cgcgttctga acgctcaccg
     9421 atgacaatac gtgacggatg aaggttatcg taaagggctt taccctcacg gagaaattcc
     9481 ggggagaata taatattttc agtacgatat tttttatgca tcgctgcggt aaaaccaacg
     9541 ggaaccgttg atttgatgac cataaccgca taagggttta tctcaactac gtc
//
LOCUS       DAPQZD010000181         9544 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPQZD010000181 DAPQZD010000000
VERSION     DAPQZD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.183"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(284..496)
                     /locus_tag="RO454_004229"
     CDS             complement(284..496)
                     /locus_tag="RO454_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935976.1"
                     /translation="MNGFRNSSRNGQVWCWQRAGSRAVILEVSGRWMEAAEGWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(629..1189)
                     /locus_tag="RO454_004230"
     CDS             complement(629..1189)
                     /locus_tag="RO454_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDY0935977.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEET
                     YAAERLDKIRRQNRIKAELQAVLDEK"
     gene            complement(1244..1990)
                     /gene="traX"
                     /locus_tag="RO454_004231"
     CDS             complement(1244..1990)
                     /gene="traX"
                     /locus_tag="RO454_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDY0935978.1"
                     /translation="MTTDNTNTIRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLVGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGHILFAFAVVAQVLTWCETRSGWRTAAAILLMAQWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(2010..7280)
                     /gene="traI"
                     /locus_tag="RO454_004232"
     CDS             complement(2010..7280)
                     /gene="traI"
                     /locus_tag="RO454_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDY0935979.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIRETVGEDASLKSRDVAALDTRKSKQHVDPEIKMTE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVEKALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDQRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGESVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMVENKPDLPDDKTELAVRDIAGQERDRTATSERETALPESVLRESQREREAVREV
                     ARENLLQRLLQQMERDMVRDLQKEKTLGGD"
     gene            complement(7280..9451)
                     /gene="traD"
                     /locus_tag="RO454_004233"
     CDS             complement(7280..9451)
                     /gene="traD"
                     /locus_tag="RO454_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDY0935980.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDDKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARKRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQIASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMA
                     AYEAWQQENHPDTQQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         1917 a         3026 c         2372 g         2229 t
ORIGIN      
        1 tgaaaagata aataatacca gtggaatata tttcctcatg ttattctcca tatcagataa
       61 atagcacctg tatttcaggc atcctgaata ttgcagcaca cactgaataa caccttattt
      121 tgtgaaatta atcacaaaat ataaaatcac gacttaatac ggaaaaaact ctgtgctact
      181 cagaactgtc cggtcatact gcccgaccgg atattaaaaa aagcaaatct catgcattat
      241 tcagccttct gaatccctgg agtaatgaag gctgcttttt tcgttacacc cttccccggc
      301 agcgccggtg gcagtgttca gccctttttc gggcaaactg ctgccagtct gtccggggcg
      361 caacacaggc agcccgtcgc catccttctg ccgcttccat ccagcgtcca ctgacctcca
      421 gaataacggc ccggcttccg gcacgctgcc agcaccagac ctgaccgttc cgtgaactgt
      481 ttctgaatcc attcatcttt atcactcctc tttaacccgt cagtattatg gtgttaaata
      541 atctactgaa ttggcttcct ttcgggtacg gaagttattt ccttttaagc cttatttccc
      601 tgcctcctct ctctgaggga ggcttttttt atttctcatc aagcacggcc tgaagttctg
      661 cctttatccg gttctggcgg cggattttat ccagacgctc tgccgcatac gtttcctcct
      721 cctgagaaat atgctccgtc acataccctt ccgtgtcata ccggcaggca ccggctttca
      781 tggcacacag atagctttct gaacgggtga tggccttcag cgccctgcgc agttttttat
      841 gcgagagcgg gatattccgc tgcgccacgt cttccagtaa cacgtcccgg ataccgcagg
      901 ccagaagccg gggcgtgtca ccgtcaaata atcccggcca ccagggcttc agggtattca
      961 cggcctcatc cagtgagggc aggttcagat aaatggacag cgcctgtctg gcctgcgctt
     1021 tttttgccgc cagctctgct tcccgggccg ctttctcggc cagtttctgc tttttcacct
     1081 tccattttgg tggcgtggtg acattgatga tggttttccg gctgcggaca ggcgctgttc
     1141 cctctgtctt ccgcttcagt gtcagtaccg gtcgcttctg ctctgtcatg ctgtgttctg
     1201 cctctgacga atcatcctct tgcggccaaa gataccacac ccgtcacagc gccagaacgc
     1261 ccagcacagc cagatgacag gcatagaacg tcgggaaaaa gtctccgggc cagaaacggg
     1321 gtaatgattt tcctgcacac gacaccagac caacggtcag caccgtcatc acaagaccag
     1381 ccaccgccgc tgcatcactg gtggcaaggt taagcgccgg aataacagcc agcaggcagg
     1441 cgaccagcgc cagacgttcc gctctgtctt ccgcccggta cagccggtgg ctgactgcca
     1501 gcatcagcag cccggcaatg ccgtaactgg tgccggacaa aggcccccac tgggccatca
     1561 ggagaatggc cgctgctgta cgccacccac tgcgcgtttc acaccacgtc agcacctgtg
     1621 ccaccactgc aaaggcaaac aggatatgcc cctcatacca gggaaagcct gccagataat
     1681 atgcgaactg ggcaataatt ccccatcccc acagccggtt aatggccggt tgccggatat
     1741 gcgcatgacg ggacagattc agcccccaca ccagggcaaa aagcggaaag gccccacgcc
     1801 cgaccagaaa catccattcc tgcttcagct ggaatatcag attgatgtgg tccagtacca
     1861 tcagcaccag tgccaccgtt ttgatgatat cccgctgtcc gggtgaccag accagaaatg
     1921 actgcaacca ggtatcggtc cgtgcagcca gtgaatcgtt acgtatcgtg ttcgtgttat
     1981 ccgttgtcat cagtgtttgt cttcctgtat cagtctccgc ccagggtttt ctctttctgc
     2041 aggtcacgaa ccatatcccg ctccatctgc tgcagaagcc tctgcagcag attttcccgg
     2101 gcaacctccc ggaccgcttc ccgttcccgt tgtgattcac gcagcacact ctccggcagt
     2161 gcggtttccc gttcagaagt ggcagtccgg tcacgttcct gcccggcaat atccctgaca
     2221 gccagctctg ttttgtcgtc cggcaagtca ggtttgttct ctaccatttt cctgacaatt
     2281 tcatctgcgc ggcgttccgt ttcacggcgg atggcctctt cagcctgccg ctgtgccagt
     2341 acctctgccg tgaccgattc accatttccg gctcccggct ggacgctgtt atccggaata
     2401 tccccccaca cgcggccacc ggtgatggca ccgggattcc acggacggcc ttcaccggcg
     2461 attcttacca ccacaccact gtcgggatta tcacgggcaa tccggacgcc atcctgcata
     2521 ttatcagcca gcaggctctc tccgttacgg ctgccctgca gggccacgaa ctgcgcatcc
     2581 ccgctgccct tcacacggcc ttcaccgctg aatccccgca gcccgtttcc gtcatccgtg
     2641 gtcagcgggt tcagccagat accggcggac ctgccgttac ggtcaaaagc cggcagtgcc
     2701 acatacggtt gcggatattt acgtcccgga gcaataaacc gtgcaggact gtctcccccg
     2761 gccagccccg cctgacggag aacggcacgg cctgccgcca cgtcccgcag ttcccgtgcc
     2821 gtactgaaca gccgctgcgc attcatgacc tcccggtccg gtttcggctc cagcacatcg
     2881 tgggcagttc ctttctgtac ggcattgtta atggcatccg tccagccctg acggttatcg
     2941 gtgtacacct gcacatgctg cttcatacgc gacagggcca cgtaggctga ctcaaagccg
     3001 gccatctgtt tccggttacc ttccgtgcct tcaagcgcga tggcaaaggt ttcactcgcc
     3061 ccctgcgcac cgtgggcagt gatggcatag gccaggtcaa tatgttgctc tgcccgctcc
     3121 tgaccggggc gaatcacccg ggtctgctgt ccgtccgaca gcgtgacact gtcaccggaa
     3181 accgctgtca ccgtccagac gctgttggcc acataaccgc gctcccggtc actcttcgtg
     3241 aagcgcatcc tgtcgccggt ccccacccgg attttgtctg gtgtgtacag tgtgacacct
     3301 tcagccaccg cctcccgggg cgaaatcagc cgcgtgttac cctccgcatc ctgcagggtt
     3361 atcagcccgt catccttgct gataccggca atccggtgat acacattatc caccagggca
     3421 agtgcgtccc gatgtgtctc ccaggtggag agacgacgca gctccccgtc acgtatattc
     3481 gctgtattca ggacaggcac catgacctgc tctttcccca gctcaccggc cttttcccgt
     3541 gcatcatgaa tcatgctgtt cagtacgcgc tggtcctcat tcaggtgcgt gacaatcagc
     3601 gtctgctccc gtgcttccgg tgtcctgccg gtatagtcgc ggacaatggc ttcatacagt
     3661 gtcatgggga tatccgggaa agcctcgcct ttcagcatcg ccttctgctg cgcttctgcc
     3721 agtttcgctt cctggctgtg actgaactcc gtcacggagt gctccggtgc ccatgcgccc
     3781 tccagacgtg gcacctgaga cggtttcaca ctctcaagcc cggacagtgc cttttccaca
     3841 tcccggttaa tcaggctgta taccgcctcc cgcagttccg gcgtctgacg cacaatctcc
     3901 ttcatgatgg ccacatcggc agcactgcgc gtctgctgga gacggaaagg ctgaccgggc
     3961 gcgatggcct gcagctggtc cgtgtcaccg ctggccacag cacgaccgcc accggccgca
     4021 atcagggcgt atgcccgtgc catgtcggta ttgcccacca ttgagctctc atcgagcagg
     4081 aacagcgtgt tgctgaaatc cggcgtttct ccgctgcgct gctgcagctg cgtgtcatgc
     4141 agaaaggacg ccagtgtctg tgcatccacg ccggcgctgc gcatctcccc gaccgcacgg
     4201 tgcgtgggcc ccagcccaac gacacgggga cgctcactct ccggcagcat gttcacggct
     4261 gacatcaccg cccggaactg tgtggtctta cccaccccgg catacccctg caccaccgtg
     4321 aaacggtcgg acgtttccag tatcatgcgg gtggcggcac gctgtcctga tgtcagtttc
     4381 tccatgagtt cgccaggtac tctctccatc agcggcgtga ccgcctcctt accctcgaga
     4441 atatggcgaa gaatgctctt ttctgcctca tacgacgcac gggaaaccag caggtctgtg
     4501 ccatagcctt tagccacatc cacatgcagt aaatcacccc gttttatctg cgcatcgatt
     4561 tcccgcccca gttcagtaaa actggttcct tctgcagcaa acgactttgc ctctgtcagc
     4621 aggtccacat ggctgaaggc caggttttta ctttccacca ccggaagggc cagatgaatg
     4681 gcctgctgtg ccggcgtgtg aagcccggct ttctgcagac tgatagcggt ttccagcaat
     4741 gtctcaccgg cccgcgcctt tatctgctca gaaaccaccg taaaggaggg atggcgggca
     4801 agtttttccg cagtacgggt ttcatccagt gaggaataca gccggacatc acgaccgctg
     4861 cgggccagac cgttcagggt ggcgttgtcc attgccatct gtgtgacgga ggcaaacacc
     4921 gtcgcactgt cactgacgga atgcccgggc gtttccaccc agccgttctc cagcttcagt
     4981 gccgtgaacg gtgaatcgct cacaggcagg gaggccggtt cagcccgccc cggcacaaca
     5041 accgtcatcg catcttcact gacggatgcc acctgcaggc ggtcaccgcc ggagacgcgg
     5101 agtccgggaa ttttccctgt caccctcagt cgctcgccgt ctgccaccgg cattttttcc
     5161 ggccggaaca gcgaccagct gctgtccagg gaggaaatac gcaccacctg cgtttcgccc
     5221 tgcgcatccc gcagggtcag gctgtgactc tgcgctgtca cccggtctat cacatagcgg
     5281 tcatgactgc gtgtctccgg gttccactgc tccatcacca tccccggtcg gtacatatcc
     5341 cgcagataac ggctccggct gtccagccag accggtgaca gtgcagtcat ggtcacctca
     5401 gggtgtccga gcacgccctg cgttttcagc tcactgcgaa tggcctgtgt cagtatggcc
     5461 tgttcccgta ccccgctgac ctgtgccacg ctctcttctc cggctttcac gctggccgca
     5521 aaatctccgg ccagccgggc atagcggaca ttacggtccg gttcactgat gatggtggcc
     5581 ggtcgctgtt ctcccccctg ccagcgatat gtgttcaccc cggcatcctt catggccatc
     5641 agcgcactgc ctgtaccggt tcgctgcccg ctgtcggtta tcaggacctg tacgttatga
     5701 cgtgcggcac cgtccagcag ggttaacgtc tctttcaggg agagtttttc gccctggtca
     5761 acgataacag tactgcccgg cgtgaaggcc atgccttcca gcagctgacg acgtccggtt
     5821 atcagctcac cggacagccg ttcatcctgc ttcaggttca tctgcgagcg acggtcagca
     5881 gcgataatct gcacctcccg cccctgctcc cgggccatca tgaccagttc agccacccgc
     5941 tcacgctgcc cggctgcacc gccctgcccg gacacaatag ccagcgacgg gcgatcctgt
     6001 gccagaacac tgaccgcatc gctgtaaccg gccgtccggg ggacactttt ctccggatgt
     6061 acggtcaccc ggttctgttt catgatgtca cgactgagtg cccggactga cagctcatcg
     6121 agcacatgaa ttcctgatgt gaacagcccc ttctcacggt caagggggat gagttgctca
     6181 cggctgatgg cctcatcgat accggcgcgc gcccgctcaa tcacaccggc ttccggcggc
     6241 agtatgccga ccgtcctggc cagcacgtcc gtgtacgtaa actgcacttt gcgttcactt
     6301 aatccggcaa tcgcctgtgt caccgcctgc tgcacatccg gcccgtcctg tgaagctggt
     6361 ccgggtgtct gcgtgcgggt atatgcccgt tgttccgctg cgtcacgata cgcccggata
     6421 tcgaacccgg tttccttcag cgtctgcatc cactcggtca ttttgatttc aggatcgacg
     6481 tgctgtttgg atttacgcgt atccagcgcc gccacatccc gggatttcag cgaggcgtcc
     6541 tccccgacgg tctcccggat agtctgtgag cgtccggaaa aggcctccac cggtacgccc
     6601 ggcatttccc acataccgtg cttacccacc acttccgttt catagcccag cgcctcaacc
     6661 tgctctttca gtttttcccg gtagagcctg ccaaaggcaa tctgattagc gtacacattc
     6721 tcaatgaacc ccgttttccc cactttgtca ctgctcagtg tcttccactc accgttatgc
     6781 tgcgtgacat tagctaccac cgcatgcgtg tgtaactgtg gttcctgatc gcgactggtg
     6841 tcgtggttaa acagtgccat caccagatta ccggtcagta ccgtttctga ctgtccgtcc
     6901 gtcatcaccc gtgtggaggc cagcgcctcc acctgacgaa cagcaaagtc cacggcctgg
     6961 ttatgtgcat caatcaggcg cttatcgcca cctaacatgg ccatcatgga gacacttttg
     7021 ggggcggaga aggtcaggtc gtagccggga cgatgcttat tactgccatc ctgcatgcgg
     7081 cttagatccg ctccgtccgg cagccttccc tccagaagac gggtaaaaac atccttatcg
     7141 acactgccct gcagtcccag ctgttcagcc cccctgccgg cccagcgttc tcccatgctg
     7201 cccagtacat agtaattatc cttgtcggta taatagttac cggcacttcc ggccgatctg
     7261 acctgtgcga tactcatcat cagaaatcat ctcccggctc aacatcctcc ccgcgctccc
     7321 ggtgcacatt aatgttcacc tcttcacgac gctgcatctg ctgctgggtg tccggatgat
     7381 tttcctgttg ccatgcctca taagcggcca tatccaccac ttcaccggat tcactgatcc
     7441 cgggtggcag ttgctgttcc atctcttctt ccggtttcat tttcagctcc tgctcgatcc
     7501 cccctgccgg aacattcaca cctgaatctg acttcttatc gttgatggca ggagacaccg
     7561 gctgttgcgg ctgttgcggc tgttgcggct gttcagcctg agtcacgtct tctccggaaa
     7621 caacctccgg gacatccggt tcgaagaggc tggctatctg acgaccttct gcttcccttg
     7681 cggcaagtac ggcactcagg cggttctcca tttccgggtt gatgtcacgc ggaataaact
     7741 ccggagcgac cttcggtcgt gtctgatatt tcagagagag ttttactgcc ggatacggtc
     7801 cgggcagggt gacataacag gtcagatccg gcagagactg aatgtcggaa taactgacca
     7861 gcgtctggcg ctccatatcc ttaccggtcg ataccccgtc acgtaccgga tcagcaccgt
     7921 aggaatactg ctcgctggct ttcaggtgct ctttctcacc aatctcaccg gcagcgaact
     7981 ctgcaatctt atggctggga gaacggaaaa aggcacgggt gttcatgacg tcaaacaacg
     8041 tggcagccgc tttctcaccg tagatatctt ccagctgggc ataggactgg ataccaaaca
     8101 cataacagcc accgaactta cgggcttccg gcaggatctc caccaggtca ggcagtttgt
     8161 gtaacgtggg taactcgtca cagaaaaacc acacacgacg gttacggttt tctcccattg
     8221 ccagcagacc acgaatggca atggacagcc acatggagat cactggcttc agggaggcat
     8281 gggtgtcggc gttcgatgag ataaacagcc agccgttttt ctgatcttcc cggacaccac
     8341 gcatccagtc acggatggtg aagggctcac cgttatgctc aatcccctgc aggtaacgga
     8401 tggctttcac gtagttggtc agcacagcac ggatggaaat cgccgttttc tcgattttct
     8461 cttccaccag gttggccgcc ggtgaattac gcaggaaggt acgcagtttt tcgattttga
     8521 tggaaagcag agtgtccacc agtttgctgt agctgcggtt ggggtcatta cgcatcaggt
     8581 acgccgcttc cgcaaaaatg gtacgtcctg aaccctgcca gaacgggtcc tcttttgtgc
     8641 ccatcgggat cagggtattt gccgtattat caaaatccgg ctgtgtcaga cactccttcc
     8701 ataaatccca tgcggcacag cgggcatcca gcggattcaa gattttatcg atggagggat
     8761 catagtaact tttaacaaat tcccctgaac ggtcataaat caccaccata tcaccgcgct
     8821 tacgggcgta gttggccaga cgacggataa cttccgactt accggccccg accgtgccgt
     8881 gcagacagaa gttctggatt tcagaatccc ggataatcgg caggtcgcca atccggatat
     8941 cggagtcctt gtcgtctttt ttcagcatcc gggcaacgtc tttcggattg tctgtcagct
     9001 gacgaccacc tgtgacttca ttttcgctct gttgtttacc ctgacgcccc agaatccagg
     9061 agacaacaaa gaaggtaatc aggcaaataa ccagtgccac aactgtggcc agaacgaatg
     9121 cggaccatag ctgctctccg caccaaatca tatatttatc atgcagtacc tgagcagcgt
     9181 tcatccggaa ggttttgccg taatactgga tctcatatac cggctgggac ttgattaaat
     9241 cccgcatgcc ttccagcgtg gtacaccacc agtaaataca gccgttcaca aacgtctgcc
     9301 agcttatttt tacccataaa accagaccaa cgagtatcca gaaaaaaata aacaggcagt
     9361 aaagcatgat attggcgatc tggctgaaca tgcggatacg catggacgca atctgaccgc
     9421 cctgggtcat atcctttgcg ttaaaactca tgatgatatt ccgaagaaaa aagtcagtcg
     9481 gactgattat taatgatgaa aagattaata accgttaaat tccggcttcg ccggcaacac
     9541 ttca
//
LOCUS       DAPQZD010000182         9538 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPQZD010000182 DAPQZD010000000
VERSION     DAPQZD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.184"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            456..700
                     /locus_tag="RO454_004234"
                     /pseudo
     CDS             456..700
                     /locus_tag="RO454_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(717..1388)
                     /locus_tag="RO454_004235"
     CDS             complement(717..1388)
                     /locus_tag="RO454_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDY0935981.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1535..1810
                     /locus_tag="RO454_004236"
     CDS             1535..1810
                     /locus_tag="RO454_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0935982.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(1911..3497)
                     /gene="prpR"
                     /locus_tag="RO454_004237"
     CDS             complement(1911..3497)
                     /gene="prpR"
                     /locus_tag="RO454_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDY0935983.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3736..4626
                     /gene="prpB"
                     /locus_tag="RO454_004238"
     CDS             3736..4626
                     /gene="prpB"
                     /locus_tag="RO454_004238"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDY0935984.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            4786..5955
                     /gene="prpC"
                     /locus_tag="RO454_004239"
     CDS             4786..5955
                     /gene="prpC"
                     /locus_tag="RO454_004239"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDY0935985.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            5989..7440
                     /gene="prpD"
                     /locus_tag="RO454_004240"
     CDS             5989..7440
                     /gene="prpD"
                     /locus_tag="RO454_004240"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDY0935986.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7480..9366
                     /gene="prpE"
                     /locus_tag="RO454_004241"
     CDS             7480..9366
                     /gene="prpE"
                     /locus_tag="RO454_004241"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDY0935987.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2307 a         2499 c         2583 g         2149 t
ORIGIN      
        1 gtgtctggtt taatcagagc tttattatct gcgacgttta tttttattta agagagccac
       61 ctgcaagttg ttattccacc agagagtgaa tcgcaaagca ggtcatcatt tcatctgtat
      121 atctgtatgc caaatggcat tccccctcct ttttcgccga tattttgttg tgatcgacga
      181 agtttcgctt aaaaaagcac cagcagtggt ttcgcagcta tatggtgtat aaaaaatgtt
      241 ctcatacaga tgttttgtga atgtgtgggt tgacattcat atgaaaaaaa tcataattcc
      301 atcatgtttg tgtgggggct tttctgtatc ttacgcatcg cactcaagcc tgacagaaaa
      361 tatgctgtaa ggctcatatc aaaaaccgcc attagctcat caggaagagc agacgacaac
      421 caggattgtt gtatggtacg gggttcgagg cctcgatggc ggtccaactt ttcaaaactc
      481 ttctgaatca gatcccctta ttatcatccc tgcaaagtgg aacattgcca ttgtttggct
      541 attcaggacg ggtcagacca atgaaaaagc ccacacaggg gagagtaggc tgaaatggga
      601 agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc
      661 gccgatggtt gggattcggc aaatcaggtt gattacgtag agagtaaatt atctgctcac
      721 cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata
      781 acccgactgg caacgcgttg catacgccca taagcacgac gcacagcggg caaagaaaac
      841 gcctggctta gaaagactcg ccagataatt gatgtgagca caatccccgc ccaggccatt
      901 aatcgtgccc atgttggtgt ttcggcattt aatgttactg agaaaatact gataaaaaat
      961 aaaacggttt gcgggttaga gagatcggta attaatccac ggcggaaaaa gatataccag
     1021 ggggcactaa tcggttgttg tagtgtgctc atttgcggtg ttgactggcg gcgcatgctg
     1081 caccacgcaa accataagag ataagcgccg ccgacgattc tgataagcga aaaaatctcc
     1141 tcacactgcg taattagcgt tgcaagacca aacaaaccca acccggaata aaatgcatcg
     1201 cccagcgcca cgcccagccc ggtcaacacc cccgcgcgtc gaccggaagc caggctggtt
     1261 tgtactacca caaagagatt ggctcccgga ttaaaaaaag taatcacgaa caatcctacg
     1321 gtcaggtaaa cggcgtgcaa aggatccata gtgatttcat ccataaataa gtgaagtaac
     1381 ttcatcatat ttctaccaaa aataatcggg tgcgagagag atcacaaagt gtcttatttc
     1441 cggttactgg cgtttatgcc ctgactgaac taattattaa tcaacccaat aatgtgggtg
     1501 ggtgatagtg tgataacaac tctggagccg taatatgaaa ataatctcta aaatgttagt
     1561 cggtgcgtta gcgtttgccg ttaccaatgt ctatgccgca gaattaatga ccaaagcgga
     1621 atttgaaaag gttgcatcgc aatatgaaaa aataggtgat atttcaacca gtaatgaaat
     1681 gtcgaccgca gatgcgaaag aagatttgat caaaaaagcc gatgaaaaag gggctgatgt
     1741 gttggtactg acctctggtc aaactgacaa taagatccac ggcacggcaa atatttataa
     1801 gaagaagtaa ttctgaatcc tatgtaaaca tccccgatgc gttagtttat cggtgatcat
     1861 catctatcga aatttatgcc ggataaagcg ttcgcgctgc attcggcagt tcagcttttc
     1921 agccgccgcc agaacgtcgt ccggctgata cctaaataat tcgccgctgc tgttttatcg
     1981 ccattaaatt tctccagtgc ctgttgtgct gtcagcaagc ctggaatggg agtcttcgcc
     2041 gactcgcgcg ccagttccgg cagtagcagc tgcaaaaatt gcggcgttaa atccggcgtc
     2101 gattccacac ttaaaaacag cgccagtcgt tccatcatat tgcgcagttc acgaatattg
     2161 cccggccagt cgtagtgcaa taatagaatc tgacaggtct ctaatccctg acgtaatgcg
     2221 gcagaaaaag ggacagagag tgccgccaga gacattttca aaaagctttc cgccagcggc
     2281 agaatatccg ccacccgctc gcgtagcggc ggcagttgta gacgcaaaat actcagccga
     2341 taaaacaggt cacggcgaaa ctgcccttgc tgcatatctt cttccagatt gcagtgagtg
     2401 gcgctaatga cccgcacatc caccggaaca ggctgatgcc cgccgacgcg ggtgacctct
     2461 ttttcttcca gcacccgcag cagccgagtc tgcaacggca gcggcatttc gcctatctcg
     2521 tcaagaaaca gcgtaccacc gtgggcgatt tcaaacagcc cggcgcgtcc tccgcgtcgc
     2581 gagccggtaa acgccccttc ctcatagcca aacagctccg cttccagcag cgattcggca
     2641 atcgccccgc agttaacggc aacaaacgga tgtgactttt tgccctgtcg cgcatcgtgg
     2701 cgggaaaaat attcccgatg aatcgcctgg gccgccagct ctttgcccgt ccccgtttcc
     2761 ccctcaatca acaccgccgc actggagcgg gcatacagca aaatagtctg ccgcacctgt
     2821 tccatctgtg gtgattgacc gagcatatcg cccagcacgt aacgagtacg cagggcgttg
     2881 cgggtggcat cgtgagtgtt atggcgtaac gacatgcgcg tcatatccag cgcatcgctg
     2941 aatgcctggc gcacggtggc ggcggaatag ataaaaattc cggtcattcc ggcttcttct
     3001 gccagatcgg taatcagccc tgcgccgacc accgcttcgg tgccgttggc tttcaactca
     3061 ttgatttgcc cgcgggcgtc ttcttcggtg atgtagctac gttggtcgag gcgcaaatta
     3121 aaggtctttt gaaacgccac cagcgctgga atggtttcct gataggtgac aacaccgata
     3181 gaagaggtga gttttccggc ttttgccagt gcctgtaaca catcgtagcc gctcggttta
     3241 atcaaaataa ctggcactga caggcgactt ttaagatatg cgccgttaga gccagcagcg
     3301 atgatggcgt cgcaccgttc gttggccagt ttcttgcgga tgtaggtcac cgctttttca
     3361 aagccaagct ggataggggt gatattcgcc aggtgatcaa actcgaggct gatatcgcga
     3421 aacagctcga acaggcgcgt tacagatacc gtccagataa ccggtttgtc gtcattcagc
     3481 cgtggtggat gtgccatagc gcaccgcaaa gttaagaaac cgaatattgg gtttagtctt
     3541 gtttcataat tgttgcaatg aaacgcggtg aaacattgcc tgaaacgtta actgaaacgc
     3601 atatttgcgg attacttcat gaatttatct ttaactcatt gaaattaaat gtttaatttt
     3661 attaaggcaa ttgtggcaca cccattgctt tgtctttatc aacgcaaata acaagttgat
     3721 aacaaaggat gggctatgtc tctacactct ccaggtaaag cgtttcgcgc tgcactgact
     3781 aaagaaaatc cattgcagat tgttggcact atcaacgcta accatgcgct gctggcgcag
     3841 cgtgccggat atcaggcgat ttatctctcc ggcggtggcg tggcggcagg gtcgctgggg
     3901 ctgcccgatc tcggtatttc tactcttgat gatgtgctga ctgacattcg ccgtatcacc
     3961 gacgtttgtt cgctaccgct gctggtggat gcggatatcg gttttggttc ttctgctttt
     4021 aacgtggcgc gcaccgtgaa atcgatgatt aaagccggtg cggcaggatt gcatattgaa
     4081 gatcaggttg gtgcgaaacg ctgcggtcat cgcccgaaca aagcgatcgt ctcgaaagag
     4141 gaaatggtgg atcggatccg tgcggcggtg gatgcgaaaa ccgatcctga ttttgtgatc
     4201 atggcgcgca ccgatgcgct ggcggtagag gggctggatg cggcgatcga gcgtgcgcag
     4261 gcctatgttg aagcgggtgc cgaaatgctg ttcccggagg cgattaccga actcgccatg
     4321 tatcgccagt ttgccgatgc ggtgcaggta ccgatcctcg ccaacattac cgaatttggc
     4381 gcaacaccgc tgtttaccac cgacgaatta cgcagcgccc atgtcgcaat ggcgctctac
     4441 ccgctttcag cgtttcgcgc catgaaccgc gccgctgaac atgtctacaa catcctgcgt
     4501 caggaaggca cacagaaaag cgtcatcgac accatgcaga cccgcaacga gctgtacgaa
     4561 agcatcaact actaccagta cgaagagaag ctcgacgacc tgtttgcccg tggtcaggtg
     4621 aaataaaaac gcccgttggt tgtattcgac aaccgatgcc tgatgcgccg ctgacgcgac
     4681 ttatcaggcc tacgaggtga actgaactgt aggtcggata agacgcatag cgtcgcatcc
     4741 gacaacaatc tcgaccctac aaatgataac aatgacgagg acaatatgag cgacacaacg
     4801 atcctgcaaa acagtaccca tgtcattaaa ccgaaaaaat cggtggcact ttccggcgtt
     4861 ccggcgggca atacggcgct ctgcaccgtg ggtaaaagcg gcaacgacct gcattaccgt
     4921 ggctacgata ttcttgatct ggcggaacat tgtgaatttg aagaagtggc gcatctgctg
     4981 atccacggca aactgccaac ccgtgacgaa ctcgccgcct acaaaacgaa actgaaagcc
     5041 ctgcgtggtt taccggctaa cgtgcgtacc gtgctggaag ccttaccggc ggcgtcacac
     5101 ccgatggatg ttatgcgcac cggcgtttcc gcgctcggct gcacgctgcc agaaaaagag
     5161 gggcataccg tctctggcgc acgggatatt gccgacaaac tgctggcgtc acttagctcg
     5221 attcttctct actggtatca ctacagccac aacggcgaac gcattcagcc ggaaaccgat
     5281 gacgactcta tcggcggtca cttcctgcat ctgctgcacg gcgaaaagcc gtcgcaaagc
     5341 tgggaaaagg cgatgcatat ttcgctggtg ctgtacgccg aacacgagtt taacgcttcc
     5401 acctttacca gccgggtgat tgcgggcact ggctctgata tgtattccgc cattattggc
     5461 gcgattggcg cactgcgcgg gccgaaacac ggcggagcga atgaagtgtc gctggagatc
     5521 cagcaacgct acgaaacgcc ggacgaagcc gaagctgata tccgtaagcg ggtggaaaac
     5581 aaagaagtgg tcattggttt tgggcatccg gtttatacca tcgccgaccc gcgccaccag
     5641 gtgatcaaac gcgtggcaaa gcagctctcg caggaaggcg gctcgctgaa gatgtacaac
     5701 atcgccgatc gcctggaaac agtgatgtgg gagagcaaaa agatgttccc caatctcgac
     5761 tggttctccg ctgtttccta caacatgatg ggtgttccca ccgagatatt cacaccactg
     5821 tttgttatcg cccgcgtcac tggctgggcg gcgcacatta tcgaacaacg tcaggacaac
     5881 aaaattatcc gtccttccgc caattatgtt ggaccggaag accgccagtt tgtcgcgctg
     5941 gataagcgcc agtaaacctc tacgaataac aataaggaaa cgtacccaat gtcagctcaa
     6001 atcaacaaca ttcgcccgga atttgatcgt gaaatcgttg atatcgtcga ttacgtgatg
     6061 aactacgaaa tcagctccag agtagcctac gacaccgcac attactgcct gctcgacacg
     6121 ctcggctgtg gtctggaagc tctcgaatat ccggcctgta aaaaactgct ggggccaatt
     6181 gtccccggca ccgtcgtacc caatggcgtg cgcgtccccg gaactcagtt tcagctcgac
     6241 cccgtccagg cggcatttaa cattggcgcg atgatccgtt ggctcgattt caacgatacc
     6301 tggctggcgg cggagtgggg gcatccttcc gacaacctcg gcggcattct ggcaacggcg
     6361 gactggcttt cgcgcaacgc ggtcgccagc ggcaaagcgc cgttgaccat gaaacaggtg
     6421 ctgaccgcaa tgatcaaagc ccatgaaatt cagggctgca tcgcgctgga aaactccttt
     6481 aaccgcgtcg gcctcgacca cgttctgtta gtgaaagtgg cttccaccgc cgtggtcgcc
     6541 gaaatgctcg gcctgacccg cgaggaaatt ctcaacgccg tttcgctggc atgggtagac
     6601 ggacagtcgc tgcgcaccta tcgccatgcg ccgaacaccg gtacgcgtaa atcctgggcg
     6661 gcgggcgatg ccacttcccg cgcggtacgt ctggcactga tggcgaaaac cggcgaaatg
     6721 ggttatccgt cagccctgac tgcgccggtg tggggcttct acgacgtctc ctttaaaggc
     6781 gaatcgttcc gcttccagcg cccgtatggt tcttacgtga tggaaaatgt gctgttcaaa
     6841 atctccttcc cggcggagtt ccactcccag acggcagttg aagcagcgat gacgctctat
     6901 gaacagatgc aggcagcagg caaaacggcg gcggatatcg aaaaagtgac cattcgcacc
     6961 cacgaagcct gtattcgcat catcgacaaa aaagggccgc tcaataaccc ggctgaccgc
     7021 gaccactgca ttcagtacat ggtggcgatc ccgctgctgt tcgggcgctt aacggcggca
     7081 gattacgagg acaacgttgc gcaagataaa cgcatcgacg ctctgcgcga gaagatcaat
     7141 tgctttgaag atccggtgtt taccgctgac taccacgacc cggaaaaacg cgccatcgcc
     7201 aatgccataa cccttgagtt caccgacggc acgcgatttg aagaagtggt ggtggagtac
     7261 ccaattggtc atgctcgccg ccgtcaggat ggcattccga agctggtcga taaattcaaa
     7321 atcaatctcg cgcgccagtt cccgactcgc cagcagcagc gcattctgga ggtttctctc
     7381 gacagaactc gcctggaaca gatgccggtc aatgagtatc tcgacctgta cgtcatttaa
     7441 gtaaacggcg gtaaggcgta agttcaacag gagagcatta tgtcttttag cgaattttat
     7501 cagcgttcga ttaacgaacc ggagcagttc tgggccgagc aggcccggcg tattgactgg
     7561 cagacgccct ttacgcaaac gctcgaccac agcaacccgc cgtttgcccg ttggttttgt
     7621 gaaggccgaa ccaacttgtg tcacaacgct atcgaccgct ggctggagaa acagccagag
     7681 gcgctggcat tgattgccgt ctcttcggaa acagaggaag agcgtacctt taccttccgc
     7741 caactgcatg acgaagtgaa tgcggtggcg tcaatgctgc gctcgctggg cgtgcagcgt
     7801 ggcgatcggg tgctggtgta tatgccgatg attgccgaag cgcatattac cctgctggcc
     7861 tgcgcgcgca ttggtgctat tcactcggtg gtgtttgggg gttttgcctc gcacagcgtg
     7921 gcggcgcgaa ttgatgacgc taaaccggtg ctgattgtct cggctgatgc cggggcgcgc
     7981 ggcggtaaaa tcattccgta taaaaaattg ctcgacgatg cgataagtca ggcacagcat
     8041 cagccgcgtc acgttttact ggtggatcgc gggctggcga aaatggcgcg cgttagcggg
     8101 cgggatgtcg atttcgcgtc gttgcgccat cagcacatcg gcgcgcgggt gccggtggca
     8161 tggctggaat ccaacgaaac ctcctgcatt ctctacacct ccggcacgac cggcaaacct
     8221 aaaggtgtgc agcgtgatgt cggcggatat gcggtggcgc tggcgacctc gatggacacc
     8281 atttttggcg gcaaagcggg cggcgtgttc ttttgtgctt cggatatcgg ctgggtggta
     8341 gggcattcgt atatcgttta cgcgccgctg ctggcgggga tggcgactat cgtttacgaa
     8401 ggattgccga cctggccgga ctgcggcgtg tggtggaaaa ttgtcgagaa atatcaggtt
     8461 agccgcatgt tctcagcgcc gaccgccatt cgcgtgctga aaaaattccc taccgctgaa
     8521 attcgcaaac acgatctttc gtcgctggaa gtgctctatc tggctggaga accgctggac
     8581 gagccgaccg ccagttgggt gagcaatacg ctggatgtgc cggtcatcga caactactgg
     8641 cagaccgaat ccggctggcc gattatggcg attgctcgcg gtctggatga cagaccgacg
     8701 cgtctgggaa gccccggcgt gccgatgtat ggctataacg tgcagttgct caatgaagtc
     8761 accggcgaac cgtgtggcgt caatgagaaa gggatgctgg tagtggaggg gccattgccg
     8821 ccaggctgta ttcagaccat ctggggcgac gacgaccgct ttgtgaagac gtactggtcg
     8881 ctgttttccc gtccggtgta cgccactttt gactggggca tccgcgatgc tgacggttat
     8941 cactttattc tcgggcgcac tgacgatgtg attaacgttg ccggacatcg gctgggtacg
     9001 cgtgagattg aagagagtat ctccagtcat ccgggcgttg ccgaagtggc ggtggttggg
     9061 gtgaaagatg cgctgaaagg gcaggtggcg gtggcgtttg tcattccgaa agagagcgac
     9121 agtctggaag accgtgaggt ggcgcactcg caagagaagg cgattatggc gctggtggac
     9181 agccagattg gcaactttgg ccgcccggcg cacgtctggt ttgtctcgca attgccaaaa
     9241 acgcgatccg gaaaaatgct gcgccgcacg atccaggcga tttgcgaagg acgcgatcct
     9301 ggggatctga cgaccattga tgatccggcg tcgttggatc agatccgcca ggcgatggaa
     9361 gagtaggtcg gataaggcgc tcgcgccgca tccgacaccg tgcgcagatg cctgatgcga
     9421 cgctgacgcg tcttatcatg cctacaggcc tgaaccgtag gtcggataag gcgctcgcgc
     9481 cgcatccgac accatgcgca gatgcctgat gcgacgctga cgcgtcttat catgccta
//
LOCUS       DAPQZD010000183         9500 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPQZD010000183 DAPQZD010000000
VERSION     DAPQZD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.185"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            97..891
                     /gene="hyxR"
                     /locus_tag="RO454_004242"
     CDS             97..891
                     /gene="hyxR"
                     /locus_tag="RO454_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDY0935988.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            1045..1806
                     /gene="hyxA"
                     /locus_tag="RO454_004243"
     CDS             1045..1806
                     /gene="hyxA"
                     /locus_tag="RO454_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDY0935989.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYLDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDCYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1848..2924
                     /locus_tag="RO454_004244"
                     /pseudo
     CDS             1848..2924
                     /locus_tag="RO454_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2946..4139
                     /locus_tag="RO454_004245"
     CDS             2946..4139
                     /locus_tag="RO454_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0935990.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            4323..4991
                     /locus_tag="RO454_004246"
     CDS             4323..4991
                     /locus_tag="RO454_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935991.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5234..5929)
                     /locus_tag="RO454_004247"
     CDS             complement(5234..5929)
                     /locus_tag="RO454_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDY0935992.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5922..7349)
                     /locus_tag="RO454_004248"
     CDS             complement(5922..7349)
                     /locus_tag="RO454_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDY0935993.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(7360..8079)
                     /locus_tag="RO454_004249"
     CDS             complement(7360..8079)
                     /locus_tag="RO454_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDY0935994.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8605..9459)
                     /gene="rclR"
                     /locus_tag="RO454_004250"
     CDS             complement(8605..9459)
                     /gene="rclR"
                     /locus_tag="RO454_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDY0935995.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2583 a         2268 c         2170 g         2479 t
ORIGIN      
        1 atattccaag aaaattaaat attcaggcta ttaaaccaaa cctgacattt tgaatcaaaa
       61 tgtcaggttt tgtagcaatg gcgtggacga gtctgaatga agaagcctct ggttattatc
      121 agtgcctgtc agttcaccag gctggcactg gagtctctca tcccaacgga caggtatatc
      181 gtgcgggtgt acagcaacgt cactacagag gttgaattcg ttttaaagac ctcctgtggc
      241 tacctgttag ctgatatccc ttcgcttcct tcgggggagt tggttcttct ggcttgtctc
      301 gcccgtttgc ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga ctccgactgg
      361 ttcgataatg ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg caccatgctg
      421 acagcgagtc gttatcagaa tcaaattatc cgctggttac tgcgtccttt gcccggcgat
      481 atgagcgatt tctggctcct gactgcacgg gagttgagcg tgcttaagat tctgatgcag
      541 ggcatgagtt tcagtgagat tgcccgttat gaacaacgca gttcaaagac cctgcatgcg
      601 attgccacac gggctttaat gaaacttgga ctgagaacac tgagcgattt caggctgctc
      661 tatacgggat gcggtgacag cagagtgaaa cggaatatac ggctacatgc aaaatatatc
      721 ggacaacatt ccgctcatcg actcactcag tatgtgcaga acatggtatc cggaatcctt
      781 tatccagaga aagttgccgt tggtgaatca ataaattctg acgccatttc cggaaaagta
      841 ctttccggac aaagaaaaaa tatatctaaa aaaagaccag gattgttatg acacagcagc
      901 gttgttacta ccacccactg cggcgcacac ggactcgcta cagtgtagga gccaaaatat
      961 ttctactgtt ctgggctgca ggattgggag gcatgctgta cagcctgtac agcgtgctgg
     1021 cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt tctgtccctc aggaattgta
     1081 ccagcgcagt tcgcagctct atcaaccggt tctgtcggca atgttacctg cataagaaca
     1141 gaagaggagt tacgcaataa attgcggcac aggccacaga gtgtcgtgat atcggcaggg
     1201 cgacctgccg aatgcgctga aatgtggttc cgattttatc tggatcactc attcgtggtg
     1261 gtgttgtgcg tcgcgccctt ttttctgcca ccggatgtga gtatttctgg cgttctcaaa
     1321 aacctgaggt tactgaaacc aggaatgtca gtagagcgtg tcatcagtat tgcaaatgcc
     1381 tctggtgggt tttcaggact gaaacatgca gaaaatttgc cagttatgga ctgttactct
     1441 gtctttatga aagaggtgaa taaccgcaca aagacaatcg tcatgtcaga acgcttcccg
     1501 gaaaagcaaa agaaggtgtt gtcgttgctt cttgccggac acagctggga atacagtgcg
     1561 cagtttctca aaacggggat aagacagata tggcttgccg agcagtcgct aaaaaagcgt
     1621 tgggggatac cggacagcat gtcattgcgg gaagccctgc tgctgagcag caataatttt
     1681 cacggtgatg gaaatgcatt ggaaacaacg aatgccatga cattgcgtga aaacggaaat
     1741 accaacaggt attccgtggt ggtaaatgca gggacgcagg ctttatctct gcataaatat
     1801 aaataagtgg gaaattgaaa ttagttacca gaatggagaa tttcttcatg aaaaacagta
     1861 aggcatttta ccgcagcgca ttagcgacag ctattgttat ggctctttct gcaccagcat
     1921 tcgctgctga taacgcggta tcaactacac cggttacatt ggataaagat aagacaactc
     1981 tggaacaaga tgttgttatt agtggtacta cacagattac agccgcaaca attaatgcga
     2041 aagataaaga ccttaatgtt actttgggtg gtcatgatat taccgccaca tcaacggtag
     2101 accaaggttt cgttgaaggt gtaaaagtta gcggtgacaa aaatgttgtg attaatgcta
     2161 caggctccac catcacagct caaggtgaag gtacctatgt ccgaactgca atggtcattg
     2221 attcaactgg cgatgttgtt gttaatggcg gtaactttgt tgcaaaaaat gaaaaaagta
     2281 gtgcgacagg tatatctctg gaagcgacca cgggaaataa tttaacgctc aatggcacaa
     2341 ccataaatgc tcaaggtaat aagagttcca gcaacggctc tacggcaatt tttgctcaaa
     2401 agggtaattt gttgcagggt tttgacggtg atgcaaccga caacatcacc cttgctgact
     2461 caaatattat taatggtggg attgaaacaa tagttattgc caaggagaat aagggaattc
     2521 atacagtcaa cctgaatatt aaggatggct caataattgg ggcggctaat aataaacaaa
     2581 caatttatgc ctctgcttcg gcacaaggcg caggttcagc aacgcaaaat ttaaatttgt
     2641 ctgttgctga ttcaaccatc tactctgatg tcctggccct ttctgaaagc gagaattcag
     2701 ccggtaccac aacaaatgta aatatgaacg ttgcccgctc ttactgggaa ggtaatgctt
     2761 atacgctcaa tagcggcgat aaagcgggta gtgatctgga tataaatctt tccgatagtt
     2821 cagtctggaa aggcaaagtt tcaggggcag gagatgccag tgtatctctg caaaacgggt
     2881 ctgtctggaa tgttacgggt tcctcaactg ttgatgctct ggcagtaaaa gacagtacgg
     2941 ttaatatcac gaaggctaca gtcaatactg gcacgtttgc ttctcaaaac ggcactctga
     3001 ttgttgatgc ctcttctgaa aacactctgg atatcagcgg aaaagcgagc ggtgacttga
     3061 gtgtttacag tgcgggttca ttggatctta tcaatgaaca aacggcattt atttctaccg
     3121 gcaaagacag cactctaaaa gccacaggca caacggaagg tggtctgtat caatatgacc
     3181 tgacacaggg agctgatggt aacttttatt tcgtaaaaaa cacgcataaa gcatccaacg
     3241 ccagctccgt gattcaggca atggcagctg ctccggctaa cgtcgctaat ctgcaggctg
     3301 acacgctctc cgcccgtcag gatgctgtcc gtctgagcga aaatgacaag ggtggcgtat
     3361 ggattcagta ctttggcggt aaacagaaac ataccaccgc gggaaatgca tcctatgacc
     3421 tggatgtaaa tggtgtaatg ctgggtggtg atacccgctt catgactgaa gatggtagct
     3481 ggctggccgg tgtggcgatg tcttctgcga aaggtgacat gactaccatg cagagcaaag
     3541 gtgacactga aggttacagc ttccacgctt acctgagccg ccagtataac aacggtatct
     3601 tcattgatac tgctgcacag tttggtcact acagcaacac ggcagatgtt cgcctgatga
     3661 atggtggcgg taccatcaaa gctgacttta acaccaatgg ttttggtgcg atggttaaag
     3721 gcggttacac atggaaagac ggtaatggcc tgtttattca gccatatgcc aaactgtctg
     3781 ctctgactct ggaaggtgtg gattatcaac tcaacggcgt ggacgttcat tctgacagct
     3841 ataactctgt gctgggtgag gccggtacgc gcgtgggtta tgacttcgct gtgggcaacg
     3901 cgaccgttaa accttatctg aatctggccg cactgaacga attctctgat ggcaacaaag
     3961 tccgtctggg tgatgagtct gtcaatgcca gcattgacgg tgcagcattc cgcgtgggtg
     4021 caggtgtaca ggctgatatc accaaaaaca tgggagcata tgcaagcctt gactacacca
     4081 aaggtgacga cattgagaac ccgctacagg gtgtagttgg tatcaatgtg acctggtaag
     4141 aataaagcag tcggttcccc tgtcgactgt tataccccct gcaggcagca gtatgctgcc
     4201 tgcaggctat aaatatccag tactcatgat gctggctttt tgctgatatt taccctgaac
     4261 attcctgata cagactatta atacaacctt atctgcgaca gatttcattt tctggatata
     4321 ttatgaatga acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga
     4381 aaaagattcg tgttatcctt tttattatag ctatctgtat ggcaatggtg ctgttgtttc
     4441 tgtatatcaa taaagataat ataaaagtga gttacagcct aaaaataaat cagacaacac
     4501 caggcatact tgttagctgt gatagcaata ataactttgc ctgtcagacc acaatgactg
     4561 aagatgttat tcagcgaatt actacatttt ttcagaccag cccagatgtc aagaacagag
     4621 aaataaagct ggaatggtca ggagataaga gagatttacc aactgctgaa gcggaaatat
     4681 ctcgcgtgca ggcgtctatc attaaatggt atacgtcaga ataccataat ggcaggcaag
     4741 ttctcgatga gatacaaacg ccttcagcaa ttaacagtga gctttataca aaaatgatat
     4801 atctgaccag gaactggtca ctgtatccga acggtgatgg ctgtgtcact ataagctcac
     4861 cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagta
     4921 ttgtgatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact
     4981 ctgggcagta ataaacagca ttctgacaga gagtgcctgt ataaaactat agtgacagtg
     5041 accgggaagg gagcccgtct tcaggaatgg attcctgtct ttttaactgt aaatatcctg
     5101 gcattattaa tgtcaggata tttacagaag agcagcataa ccatgacgga acggggttaa
     5161 gtcaggttgt ccttaacgct attcacaacc tcgtataccg ccttcaggtt tatgtccaga
     5221 ctacatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc ggaccatgaa
     5281 ccccgacgac tttgataagc tcaatgtccg ccgttgaact gggaccactg atgatgttaa
     5341 tacatgacgg cattcgttca ccggcctgcg ctttctgatg caatttttct gcaagttgcg
     5401 ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagattcc gggagcaggc
     5461 tcaatgaacg cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
     5521 catattcagc atatacgacg cccactatag cctgctctgc ttgtgatatg ttctcaatac
     5581 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttcg ctaatcccca
     5641 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
     5701 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ttcacagcgc gtcaacataa
     5761 catcgctggc aaactggata aacgcgtcac agcgctgttg ttgggtaagt tgggtaagcc
     5821 gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
     5881 gacccagtgc ctgagcaacg ttattcaaaa attcgctccg attatccatt cttattctcc
     5941 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
     6001 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
     6061 aaccagcttg ccgcatgagc accagccatc atcccaactt tccacaatcc tggatgacta
     6121 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca
     6181 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg
     6241 ctgtcacaag cggtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
     6301 ccaagtaacg gggaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
     6361 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcaacg aatacagcgc
     6421 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
     6481 tgaaactctt caggaccatc aacgtgcccc gcttcgcgtg gtcctgtcag ccaggtgttg
     6541 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
     6601 tctgcaaacg tgggagcaat acgctccatt cccatcactg caatatgcgt tttaggcagc
     6661 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc ggtctctgcc
     6721 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
     6781 tgccgaatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttcg
     6841 tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagag
     6901 ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatc
     6961 ccggcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga tttagatttc
     7021 actaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt ggcgtcttct
     7081 ttggtttttg caaaatagac gtgaccgccg ttttgtgtca ctttttctga gagctggtac
     7141 agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac
     7201 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
     7261 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
     7321 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcagcacttc
     7381 agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgtccac tgatgttcag
     7441 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcgcaac
     7501 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
     7561 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
     7621 cagcgtaagt ggttcgtcct tcacccccag cttacgggcc aggctacaag atgggtgata
     7681 caccgctctc ccttgcaaac tggcacctac atcgactacc cctaatgtat taacaataaa
     7741 agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc attcaggttc
     7801 atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat
     7861 aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
     7921 tttgatataa ccactattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
     7981 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
     8041 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
     8101 tgcccattta tgcccgatgg ttccctcctt ccgggggatc aatcatcaat aaatatggcg
     8161 ttctttttta attggctagc aacataccac ctctcatttg tatgacaaat gataatcttt
     8221 catctaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttata
     8281 gggaaatcga cgatcatttt tggcataaga aatccattta aaaaggctac atcccttata
     8341 cctattcaga gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa
     8401 tcaccgacaa taatcacgca tcaagtaata gtcgggattt tatttaattg aagcaattta
     8461 gtttgcattt ttatttaaaa actgagctag agtccatcaa aaaaatatat aaaacccaaa
     8521 accaccgtta gaaatagaaa tagttgaaag gctcacaact cccggaatgc ggattatctt
     8581 tgccctgatt tctggcggtt ttattcaggg tgcaagctgt ctcacccttt cccgatattc
     8641 tcccggagta caaccaaact cgcggacaaa cgccttgtga aaagatgatt cactggcata
     8701 gcctactgac tcagcgatca ccacaacagg gagcatttcc cgggaaaaca tctgggccgc
     8761 tatttgtaga cgcaactttg ttaatacagc cagcggcgtg gttccggaaa catcacggaa
     8821 aagctgggca aaacttgccc gggacatgtg ggcgatgctg gccagcgatt cgacggtcca
     8881 ggcgtgtcca ggcatttcca tcatttgctg tattaccgca ccaagacgtg gatgcagaag
     8941 caaactgaga atgtttttct ctgtattaac ctgtgcaatc cactcacgca ccgcgagggt
     9001 aaagaatgta gcgcagatct ggctacacag agcatccact cccggcattg ccgatctgga
     9061 ttcctgttgt aaaaacggga tcgcctccct cagccagtta tattccacgc tatggttcac
     9121 tggtgctaca aacagcgttt ccggcaaaga cgttaaaaaa taacgcgccg aatgttgcag
     9181 ccgaagagtt ccacagacaa tacaggtcga ttcattatcg acatgactca ggcgatgagc
     9241 tgaattttgt ggtagcagga ccacatttcc cgggcgtaat gtaaaaatct ctcccgtcgg
     9301 catttccagc ttcgccgctc cttgcgttaa cgcatgccaa cgaataaccg acaactcccc
     9361 ggcaccatgt ggaagttgcc agtcacttcc taacacgcaa ttcttatcga tcgttccttg
     9421 tggagcgtta agcatcaaca aacggctaag agcatccata tcagactcct gagtggtttt
     9481 tggattacaa acaccaacaa
//
LOCUS       DAPQZD010000184         9487 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPQZD010000184 DAPQZD010000000
VERSION     DAPQZD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.186"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1326
                     /locus_tag="RO454_004251"
     CDS             <1..1326
                     /locus_tag="RO454_004251"
                     /inference="COORDINATES: protein motif:HMM:NF015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0935996.1"
                     /translation="HTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPSQFANA
                     ANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFPDVVAAFKRILAEKL
                     SDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEATGIYD
                     YYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFAKSGKS
                     TAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVHRKLAE
                     LGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRASYDLRD
                     DRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRIADLVI
                     TINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            1474..2589
                     /locus_tag="RO454_004252"
     CDS             1474..2589
                     /locus_tag="RO454_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDY0935997.1"
                     /translation="MSELKELITELDFEQWLDTEGIIYRRGGVSARGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WTPKKEEIVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLASIGLNVKVACLGEEGL
                     DPGDATPGQIIKAYYCAKPYSRQLELLSKVKGIAALV"
     gene            complement(2581..3726)
                     /locus_tag="RO454_004253"
     CDS             complement(2581..3726)
                     /locus_tag="RO454_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDY0935998.1"
                     /translation="MKYIKSSSLLALTLLFSSGFVNADNKQTLIEAATAGDTAAQSEL
                     GTNYFDGINGFDKDVVEAKKWIDLAAEKGDKVAYYALGVMYTFGEGVDKDLNKAVEYY
                     KLAGDAREGRAYNNLGAIYQKGMLGKVDHALAIKYFKLASDAGYVKASYLLGAYYQYG
                     KGVQKSYKKAFTYYKKAADQGSSDGMFALGTLYDDGLGVKRNDAEAIKWYKKAAELGN
                     ADAMTNLGIMYEYGEGVKKDYKKAAELYQAACDKEDKNGCDYIAELKESGKYRAPASK
                     AKTKSATQRLIAKSIDKGVNATFTWQGDDATFTANDGKVDCTFLKDFSEKGGNLATSF
                     VCTDNVQIILKQFRDTKNAYLAVMTDNFNTEVKSFSVNVYVTNTGSN"
     gene            3960..4373
                     /locus_tag="RO454_004254"
     CDS             3960..4373
                     /locus_tag="RO454_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0935999.1"
                     /translation="MSKNKLILTGALLSLFALVGCKPTDEKAIALGQQAIADDMKDPT
                     SVMFRKDKFVRTDHDDGSVTGFVCGELNAKNGFGAYVGYHSYVVELEMKPKGMFSKGV
                     VYKILSKSLAPNDPHDLQRYKQVYRKMCNSEAVAH"
     gene            4499..5275
                     /locus_tag="RO454_004255"
     CDS             4499..5275
                     /locus_tag="RO454_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936000.1"
                     /translation="MKDEIQKLACDIIDKTGLEISEGSRLDIIGKAVNTAMNHIAARM
                     DEIPLPGLPYLKVELHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLINKGANSAVNVIRESEYASLWDMCKSQLDLVHIVVDAGKACDGVMAEFAGKMVRIA
                     NELPMIKQEAKSYATNHVTELLAPYRLESDQRKMISWGSW"
     gene            5555..5812
                     /locus_tag="RO454_004256"
     CDS             5555..5812
                     /locus_tag="RO454_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936001.1"
                     /translation="MMVEEIRKGESLYFEDMTALIKEVEERNKQARTIKRKRDSVTIS
                     SMVKKSATSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ"
     gene            5809..7131
                     /locus_tag="RO454_004257"
     CDS             5809..7131
                     /locus_tag="RO454_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDY0936002.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESVPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRTQAKSKEYAKTGMKLKERLAIL
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            7128..7307
                     /locus_tag="RO454_004258"
     CDS             7128..7307
                     /locus_tag="RO454_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936003.1"
                     /translation="MMLGWMIAFLAVGFFIGIVVMSSCINDYIKSGVIERRGRIYRIV
                     EITNTLKEIKDDRIK"
     gene            7291..7506
                     /locus_tag="RO454_004259"
     CDS             7291..7506
                     /locus_tag="RO454_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936004.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGALK"
     gene            7652..7942
                     /locus_tag="RO454_004260"
     CDS             7652..7942
                     /locus_tag="RO454_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HDY0936005.1"
                     /translation="MSNLTSWDWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTQVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKGTTE"
     gene            8734..8808
                     /locus_tag="RO454_004261"
     tRNA            8734..8808
                     /locus_tag="RO454_004261"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8766..8768,aa:Asn,seq:gtt)
     gene            8814..8900
                     /locus_tag="RO454_004262"
     tRNA            8814..8900
                     /locus_tag="RO454_004262"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8848..8850,aa:Tyr,seq:gta)
     gene            8905..8980
                     /locus_tag="RO454_004263"
     tRNA            8905..8980
                     /locus_tag="RO454_004263"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8938..8940,aa:Thr,seq:tgt)
     gene            9205..9291
                     /locus_tag="RO454_004264"
     tRNA            9205..9291
                     /locus_tag="RO454_004264"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9237..9239,aa:Ser,seq:tga)
BASE COUNT         2707 a         1968 c         2289 g         2523 t
ORIGIN      
        1 catacaggac gcgggctgtc cgaagagttt gatgaagatt tccagaacag actgacggca
       61 tatttttgtc gtgatcacga gtttcttact cgtgcgggag atctggttgt gcctagccaa
      121 tttgccaatg cggccaatgc catattggtt aatatggttt cgggctatta ccgtatgtac
      181 aagagcgcgc cttcttcatc tgcgattctg gatatgctta agcgcgcgaa acgcgataag
      241 accatccgtg aggaactctt cccggatgtt gttgctgcgt ttaagcgcat ccttgcagaa
      301 aaattgtctg atacctcgta catggttgac caggtatcca cattcgcaaa aagtgtagcg
      361 tttgatgatg ctctgattaa agctgctgaa ctgaaagaga agggcgactt tcagggggcg
      421 atggcaatca tggccaaggt tcagcagata ggctcgaacg aagcgaccgg aatctatgac
      481 tactacacct ccgcaagtga gcgattgaaa gcgcgtgaat atgaggcttc agaggagtat
      541 gtgccaaaca gcattacaac tggactccct ctgctcgata ggttgctgta ccaaaaaggc
      601 tgggcgaagc gtgaaatggt gctcttcatg gggttcgcta aatccggcaa atcgaccgca
      661 atgggtgagt tttccataaa cgcaacgctt gctggctaca atgttctgta tctctcgctg
      721 gaggttcaca ccaccatttt atccgaccgt tttgatgcaa gattgtcgga gacagaaatg
      781 tccaagctgg tggaacggcg cgatgaggtt catcgtaagt tggcagagtt gggagccacg
      841 aaggggattg gtagtttgtg ggtggttgag cgtccgtcag gaagtatgtc accggcagat
      901 ctggaccgta tgcttaacag catgaaagcc aacggcatgg tgcctgacat ggttgttgtc
      961 gactacgcag atttgatgcg tgccagttat gaccttcgtg atgatcgcgc caacattcgt
     1021 agtatctaca ccgatttacg tgctctttat gacaagcata acgttgctgg gattacggca
     1081 tcgcagacaa accgtgaagg tggcgcgtca gaagttgcca caatgatgca cgctgccgac
     1141 aacatcgaaa aagtacgtat tgctgacctg gtaataacga tcaacaaaac cgaagaagaa
     1201 gaagcgaaag gagaggctcg tctctacttt gctggttcac gtaaccagca gggagggatc
     1261 agcattcgcg ttaaacaaaa cctcgaacaa atgcgcttca ttgagcgaat cttagacgtt
     1321 acctaaaaaa taagcgtgga gaacgcctcc acgcttgatt cattggtgaa acaacttttc
     1381 ttttgccaaa ccacaaaaga aaaacacatg agcctttatg ttatatcaac atttaggttg
     1441 gtcacaatat tgcctgttaa aagtggaatt atcgtgagcg agctgaaaga gctaattacc
     1501 gaattagatt ttgaacaatg gttggacact gaaggtatca tttatcggcg cggaggcgtg
     1561 agtgctcgcg gtcgtgaagt gaatatcaag gagtgtccgg tatgcggcag ctccaactgg
     1621 aaggtatatt tcaatctgac cagtggtgtg ggtaaatgtt tcgctggcga ccatcctgaa
     1681 gaaatacaat ttaataagct ggtgttcctc aagcactaca gcggtaaatc acggcgacag
     1741 ttcgaagaat atgtgcagaa cgcccttctt tctcagggat ggacaccaaa gaaagaagag
     1801 atagtgcttg caagcacagt cgagttagag ggaccagttg ccctccctcg tcattacgaa
     1861 ctgcctatag atggccgtct tccagactat ctggttgaac gaaacatatc gcctgaaatg
     1921 gcaaagtatt ttgacctacg atactgcgtc gaaggcaaac acgcctatgt agatccgtat
     1981 acagatcagg taaaaggaca gatattcgat atgcgaatac tcataccggt ttacgatctg
     2041 gatggggtaa tgaagacatt tcagggacga gacattaccg gtacagcaga acgtcgctac
     2101 ctctttccta tgcagcttcc agcttcaggt aagtttctct acaacggcca taatgcagtc
     2161 ggcaaacaga cggtagttgt ctgtgagggg gcgttcgatg ttatgggggt caaacgagct
     2221 atttttgacg aagaaacatt acgtgattac gtggaaccaa taggaacgtt cgggatgcat
     2281 ctatctggca acaccactca ggatgcagaa gatcagctgg gcgcgttcct gacgctcaag
     2341 gcgcgtggat tacgtaatgt gatcatgatg tgggatagtg aaaagcaagc tatacgcaac
     2401 actatggccg cagctaggcg actcgcaagt attggtctta atgtcaaagt tgcgtgtttg
     2461 ggcgaggaag gactcgaccc cggcgatgcg acaccggggc agattatcaa agcctactat
     2521 tgcgcaaaac cttattcacg acagcttgaa ctcttaagca aggtgaaagg cattgcagca
     2581 ctagtttgaa ccagtattag tcacatacac attgaccgaa aatgatttaa cttctgtatt
     2641 gaagttgtca gtcattactg cgaggtaggc attcttggta tctctgaatt gcttcaggat
     2701 gatttgaacg ttgtcggtgc aaacaaacga ggtagccaga ttgcctcctt tctctgaaaa
     2761 atccttcagg aaggtgcagt caactttgcc atcattagcg gtgaaggtag catcatcacc
     2821 ctgccaggta aatgtagcgt taacgccctt atcaatggat ttagcaataa gtcgctgggt
     2881 ggcggatttt gttttggctt tggaagccgg cgctcgatat ttgccgcttt ctttcagttc
     2941 tgcaatgtaa tcacagccat ttttatcttc tttatcacaa gcagcctgat aaagctcagc
     3001 tgctttttta tagtcttttt taaccccttc accatactca tacataatgc caagatttgt
     3061 catggcatcg gcgtttccga gttcagcagc ttttttatac cacttaattg cttcggcatc
     3121 gttgcgttta acacctaaac cgtcatcgta taaggtgcct aatgcgaaca tgccatctga
     3181 actaccttga tccgccgctt ttttgtaata agtaaaagct tttttataac tcttttgtac
     3241 acctttacca tattggtaat acgcaccaag aaggtagcta gctttaacat acccggcatc
     3301 agaggctagt ttgaagtatt taatggcgag cgcatgatct actttaccaa gcatcccctt
     3361 ttgataaata gcaccaagat tgttataggc tctaccttca cgagcatctc ccgcaagttt
     3421 gtagtactca actgctttat ttaagtcttt atctacaccc tcgccaaatg tgtacataac
     3481 cccaagtgca taatatgcta ctttgtctcc cttttctgca gcgagatcaa tccacttctt
     3541 ggcctcaact acatccttat caaaaccatt gataccatca aaataatttg tccctaactc
     3601 actttgtgct gcggtatccc ctgcggtagc ggcttctatc agcgtttgtt tattgtctgc
     3661 gttaacgaat ccagagctaa aaagcaaagt taatgccagc aacgatgaag attttatgta
     3721 tttcatattt tttgttctgt tgatatagga tgaacagttt agacgcggca agtgtcaagt
     3781 gtgcgaaaaa tggtacttaa atttagttaa tatgtcagct ttgttgattt agtttatggt
     3841 cggtaaagtt actaggaaac taataaccat aagaaatagt acgccattta attattgtgg
     3901 cactgctcgt gttttgatta actaatacct gcgataaata taaataaagg gaagccaaca
     3961 tgagcaaaaa taagctgatt ttaacaggag ctttattgtc tttgtttgct ttagtggggt
     4021 gtaagcctac agatgagaag gctattgctc tgggccaaca agcaattgcg gatgatatga
     4081 aagatcccac gtcggtgatg tttaggaaag ataagtttgt caggactgat catgacgatg
     4141 ggagtgtgac aggctttgtg tgcggagaat tgaacgccaa aaatggcttt ggtgcatacg
     4201 tgggctatca ctcatatgtt gttgaactcg aaatgaagcc aaagggcatg ttttcgaaag
     4261 gggtggtata caaaatccta tcgaagtcgt tggcacctaa tgatcctcat gacttacagc
     4321 gttataagca ggtctacaga aagatgtgca acagtgaggc tgtagctcat taatggtgaa
     4381 tgccgggcag actataagcc cggcatcttc gtatagggaa ctattgcgcc taaaatgata
     4441 agtagataca tacttagttt tttgtaagaa tactttcatc tgttagctag gagttggtat
     4501 gaaagacgaa attcagaaat tggcctgtga catcattgat aaaacaggtt tagaaatcag
     4561 cgagggcagt cgactagaca tcattggaaa ggccgtcaat acagcaatga atcatatcgc
     4621 tgctcgtatg gacgagatcc cgctaccagg gttgccttat ctgaaagttg agttacacgt
     4681 atggggcgaa ccttcttgtg cacggcgttc tgcattagtt gtttttatta gcaaagaaaa
     4741 cccgctcagt cttaaagtgc aggttggagc atggcttgat ggcagagtga tctacacaaa
     4801 taccgttttt tgtctttcaa acgacgaaac tattgaagcg gccattcaag aagcagttct
     4861 agcaatgcgc agcagcggtt tgatgaagaa taactacgaa gagtacttgc gttcgataag
     4921 tggtgaaaag acattatctc tgaaagcaga tttcgttacc ccgaaaaatc tgttggaagt
     4981 tttgattaat aaaggggcta atagtgccgt aaatgtaatc agagagagtg aatacgcgtc
     5041 tctttgggat atgtgcaaaa gccagttgga tcttgtgcat atcgttgttg atgcggggaa
     5101 ggcttgtgat ggcgtaatgg cggaatttgc tgggaagatg gtcaggattg ctaacgaatt
     5161 accgatgatt aagcaagagg ctaaatcata cgccaccaat catgtcacag agcttcttgc
     5221 cccttatcgc ttagaaagcg atcagcgcaa gatgattagc tggggaagtt ggtaagctct
     5281 ctgtgtgtcg tttttacgca aataatgata ggtaagcaca agattatttc tggcggtaat
     5341 tgtgaaagct gatttgtcaa aaatcccttc tatttcagga aataacggtt attcacttcg
     5401 ttgtgaggaa gtaaagataa acggtgagtc ggcatattgc agctattccg tttgccagca
     5461 caccattctt gccttcaaag aaaaccgtct tcctcgaact tctttccagt cgtgcgcaac
     5521 cactattaaa gcaggcaaat gcaaggcgtt aaaaatgatg gttgaagaga ttcgtaaagg
     5581 agaatctctg tatttcgaag atatgaccgc actcattaag gaggttgaag aacggaataa
     5641 acaagccaga actataaaac gaaaacgtga cagtgtaacg attagtagca tggttaagaa
     5701 gagcgccaca tcacaaacag cgatcactga cgtgtatgcg gcgttgcttg aagaaacaac
     5761 aaaagaaaca catgagcaaa tcgatcaaca tatggaggca aaacaacaat gaaaaagttg
     5821 atcgcactta agcataagct ggacgaaatg aaagctatgg gaaccaatgc aaaaaaagag
     5881 gcattggcca acatggatga ctttgaacaa agcatggttt cattgatgct caaccctttc
     5941 atccgttttg gggtaaagaa atacaaagtg gcagagccgc ttagtgagtc cgtcccaagt
     6001 gacgaaaaag ccattgatgt actgaataag ctggcctctc gcgagctgac ggggaacgca
     6061 gcaatagcag ctgttgagtc tatcgtggcg tcaatgtgcg ccgatgggca ggacgtgttc
     6121 cgtcgtttcc tcttaaaaga cccgaaagca ggtgttggga ttagcctatg caacaaggtt
     6181 tttgaaaatc ccattccgaa attcgaggtg cagctggcgt caccgtataa agaaaaaggc
     6241 gacaaatacc ccttcaagcc aaatcctaaa gccaaatggc cgatgattgg cagccttaag
     6301 ctcgatggtt tgcgagtaat ttgtgaggtt attgttgacg aggaagaggt taacttcctt
     6361 tctcgtactg gtaatccaat cacgtctctc gatcacctaa agccagcaat gctcgaatta
     6421 ggcaaactct caggccacaa acacatcttc ttcgatggtg aaggaacagc cggttcattt
     6481 aaccagtccg tatctgcatt gcgcaaaaag aacgtgcagg caattggcgc tatttatcat
     6541 gttttcgact tcttcctacc ggaatggcgg acacaggcta aatccaaaga gtatgcaaag
     6601 acaggtatga agctgaaaga gcgcctggct attctcgtgg cgttgttcaa aaacgatcgc
     6661 agtgaaggct acacacaaga cattcacctg catccgttct atatcatcca tagccacgaa
     6721 gatttcatcg aacgcttcat gaaacgcctg gacgataacg aagaagggga gatgggcaaa
     6781 gatccgaact ctgtttacga gtttaaacgt acccgcagct ggtggaagtt aaaagacgaa
     6841 gattcagaag atggtgaaat tatcgatttt gagccgggcg atccggactc tggttttgcc
     6901 aacacgcttg gaaaaattgt tattcgcctt gaaaacggtg tgattgttcg tgcgagcggc
     6961 attaagcata aatatctgga cgagatctgg aacaataaag agaagtaccg tggtcgtatt
     7021 gtcgaggttc attgtcacga gaaaacgccg gatggcagct tacgccaccc acgactgaaa
     7081 tggccgcgtt gcttacgcga tacagaagat cgaatcggag ataaagaatg atgctcggct
     7141 ggatgattgc atttttagca gttggttttt tcataggtat tgtggtgatg tccagttgca
     7201 tcaatgacta cattaaaagc ggtgttatag aaagacgcgg tcgtatttat cgcattgtag
     7261 aaataaccaa caccctgaag gagattaagg atgatcgtat taagtaaacg ggagaaggaa
     7321 acgcttcatg aaatcagtaa gtggccggag ttccctgagt actggaagcc taaaacgcga
     7381 gctaagttag agcgtttagg gttggttgca aacgtttctg aaacgtggtg ttcggccaac
     7441 taccagttaa ctgataaagg gaaagtattg ctacagcaat tagtagaatc aggagcgtta
     7501 aaatgattcc atacatctca ttagctttta tgggtggctt ccttatcggc ttcggcatct
     7561 gtcgtgactt aattaagcag gaacttaaaa ccaaaacact gtgcatcgga aagcgtgtgt
     7621 atcgggtagt tcatgaaaca aaggttagaa aatgagcaat ttaacttctt gggactggtg
     7681 gttggccacc tatttcttag cggccggagt cgcattcgcc ttttacgtag gtcagttagt
     7741 cgtaaaactg ttgctgatta aatttgctag tcataaacgt atcgatgatg gtctgtggcg
     7801 tcttggcacc caggttgaaa ctcgctacgg gcaacttaag gagaacgaaa ccattactat
     7861 ccaagcgaaa cgattcactg ccaccatcac aagaacacct agtcgtagag tggccttgat
     7921 caaaaaaggc acaaccgaat aaaaacatgg tgataagtat ttacttactt atcttttatg
     7981 tataagatga ctttgttttc gttgagacgc gactgtttga acttaaatac aagtgcaaac
     8041 gaagaagtct atctggcagt agcctaataa gccaaacacc agcgaggtca gtttccagcc
     8101 tcgttaccga aatgggacac actgagcgag tgtgattgca gaacgcagga tagggcatag
     8161 acagccaaac cactatgccc tattcgatga agtaacagaa tgggcggttg gtatattttc
     8221 aactccatat gactcccgga ttcttagcct ctgaccgccc atcctgttac gtcattttgt
     8281 tcaattatgt cgtttatact gggttaaaaa gcggcgacgt agcccggctg gtatggttag
     8341 ccagcacaca acgttgaggc cattacattt ttatcaattc taaggttcta ttcacagaga
     8401 taccggcgag cgttgatatg taacatgttg ggcaaacatt caatcggagt agtggcctca
     8461 acgttgtgaa gacaggattg ttgtgtaggt ttaaccactg ttgccattgg tgcctgtttt
     8521 cacaacaaat gattccatac atcacattgt ataaattaca aagtaggtgc tgtcctcaaa
     8581 aaacatcatc tacttaaaga ttttgccttc tattgagcga agtcgaaaac gtctggcact
     8641 aacgaaaagt gcaagtagcg gtgcgtttcc tggcagaaac taacaccgcc gcgattggca
     8701 ctgttgagta ataaatactg gcagtgctga attgatggtg tagctcagcg gtagagcagt
     8761 tggctgttaa ccaactggtc ggtggttcga atccacccac catcgccaat ttaggggagt
     8821 tagtccgtag aggtagcggt gtagactgta aatctactgt cattgcgact cgggtggttc
     8881 gactccatca ctccccacca aattgccggt ttagctcagt tggtagagcg cctgccttgt
     8941 aagcaggatg tcagcggttc gagtccgcta accggcacca atacaacgat aagatcatta
     9001 cggttaatcg tcgttcatgt gcacaatgac tggtcgaaag gtagtgatct taccgttgtg
     9061 gtggattcgc agactgatgc gatagctttc ttggatgtta gggaaatgcg gccgcaaatc
     9121 ttccctttcg cgagcggtga tcgtccgtag tcctaattgg ctgcaccgta tgccggagat
     9181 tcagtaccgg ccaccacaac ggccggagag tagggagcat ggtgctcaag cggtcttgaa
     9241 aaccgtccca ttgcgcaagc gatgatggtt cgattccatt actctccgcc agacacagcg
     9301 ttgagcggtt tggcctttta atcaactcga ttaagactcc gctaacataa accagaccgc
     9361 tcaacgctgt gatagacaat tacggcagac gttcttaacc atagcttgct aacatcctag
     9421 caacactttt ttcagcgcaa aattcaaagg ggcttcggcc ccttttcttc aaaaaagttt
     9481 tataaat
//
LOCUS       DAPQZD010000185         9456 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPQZD010000185 DAPQZD010000000
VERSION     DAPQZD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.187"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(143..328)
                     /locus_tag="RO454_004265"
     CDS             complement(143..328)
                     /locus_tag="RO454_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936006.1"
                     /translation="MLKEDSYLLKALEMRGVEPTVKNAHDIYLQFSPELKRFINQYGV
                     NDSSVKAMIDDELDHLI"
     gene            complement(365..664)
                     /locus_tag="RO454_004266"
     CDS             complement(365..664)
                     /locus_tag="RO454_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936007.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFDSVHFDCGAPVVEGLECWQW"
     gene            complement(826..2859)
                     /gene="metG"
                     /locus_tag="RO454_004267"
     CDS             complement(826..2859)
                     /gene="metG"
                     /locus_tag="RO454_004267"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDY0936008.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3017..4117
                     /gene="apbC"
                     /locus_tag="RO454_004268"
     CDS             3017..4117
                     /gene="apbC"
                     /locus_tag="RO454_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDY0936009.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(4155..4544)
                     /locus_tag="RO454_004269"
     CDS             complement(4155..4544)
                     /locus_tag="RO454_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDY0936010.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            4718..5268
                     /locus_tag="RO454_004270"
                     /pseudo
     CDS             4718..5268
                     /locus_tag="RO454_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(5364..6293)
                     /locus_tag="RO454_004271"
     CDS             complement(5364..6293)
                     /locus_tag="RO454_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936011.1"
                     /translation="MSTDLSKLCADFLRQNHVSQSTEKLKASHARELVAAFFGYKSHA
                     ALMAEKTYPLVQLEEAYIFIPDIPLMNDRRSKLNGLPNDLTQSIDLARLLSDMLTSEG
                     LCGGNVWIHDTLENYIVEVLLPDCQSLIDDQLSGLMAETNAEFFDAYYDDVQIEDRGD
                     KLVAIAKTQYKGETLDDRPFCGDTLDIVVKVILPRMAGKRGFYDFELEAGGCVNDDRV
                     DPELKYGKPSQSRLAAELGITDEDLELLEWDTQENSSDDGLIYDFVLTFDKSCPPEIL
                     EMIEGLSDNLTIRVSANAFDNPYPDENMHYEYP"
     gene            complement(6788..7312)
                     /locus_tag="RO454_004272"
     CDS             complement(6788..7312)
                     /locus_tag="RO454_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDY0936012.1"
                     /translation="MTKFGKLEAHLLTRNYRHDIHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRSHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDKDAAGESLVKSFGKGFVAPDLDEL
                     QPHEVSHLIFSHSQ"
     gene            complement(7326..8177)
                     /locus_tag="RO454_004273"
     CDS             complement(7326..8177)
                     /locus_tag="RO454_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936013.1"
                     /translation="MHKSVAGEFQKEVDNTTDLLDDILSILALLEAGDWSEHCTKTEL
                     GGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSDRVEK
                     VDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAYV
                     RAKDKGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSLK
                     GGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEKY
                     GDKWEVL"
     gene            complement(8395..8895)
                     /locus_tag="RO454_004274"
     CDS             complement(8395..8895)
                     /locus_tag="RO454_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936014.1"
                     /translation="MSNKIENPVVLIHKRENHDSYAVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDEMCADEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDMARNAMIDFVDGEVGPNKNVPGLIRGAEICVSIAEQL
                     RKGVEE"
     gene            complement(9034..9414)
                     /locus_tag="RO454_004275"
     CDS             complement(9034..9414)
                     /locus_tag="RO454_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936015.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFIHIQKQADVFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
BASE COUNT         2431 a         2343 c         2144 g         2538 t
ORIGIN      
        1 aaaaatcgcg tttatatatt atcaattttc tcacttgtat aacctaaata cgcatccgtt
       61 tatcaatcca ccttccacaa acatcagctt tagctatgaa aaataacaat ctcaaagtga
      121 aaaccattat cataggaaac gattagatga gatggtctaa ttcatcatct atcattgctt
      181 taacacttga gtcattaacc ccgtattgat ttatgaaccg tttcagctcc ggtgaaaact
      241 gaaggtagat atcgtgagcg tttttcactg taggttcaac gccacgcatt tcaagcgcct
      301 ttagtagata tgagtcttct ttaagcataa attctccgtt gtctagtatg aagcgattgc
      361 cggattacca ttgccagcac tctagccctt caacgaccgg tgcaccacaa tcaaaatgta
      421 cagagtcaaa gcctacatca agtactttct gaatgttcgc aatcgcgccc tcggatatac
      481 catatcgacg caactcatct ttccagtcat cgtcctgtaa cccggcgtgg acaatgcaac
      541 cgtactcaat ttgtgaaacc caatccagct cattgcttgc gatcatgaat cggcgagcat
      601 cttcctctga tgtatgagat gagctgatca atgccacctg gtaggcttgg gtaatgttca
      661 acatttcaac cactccaaaa caagttgttt tcttatgtgg tttattatct ccatcaacaa
      721 aaggcagaaa acaaattgtt ttaggtactg tgtaaatcag aagggattgt gtctatatga
      781 aaatgcccgg cgtgttgccg ggcattgcga agagaggttg tttaattact taacctggtg
      841 gccaggttta gcaccagcat ccgggcttaa caggaagata tctttcccgc cggggccagc
      901 agccatcacc atgccttcag agataccgaa gcgcatttta cgtggtgcca ggttagccac
      961 cataatggtg tgacgaccaa tcagtgcctg tggatctgga taagcggaac ggatgcctga
     1021 gaagacatta cgcttttcac caccgagatc cagcgtcagg cgaagcagtt tgtcagaacc
     1081 ttctacaaac tctgcgtttt caatcagcgc cacgcgcagg tcaactttgg cgaagtcgtc
     1141 aaaggtgata gtttcctgaa tcgggtcgtc agccagtggg ccagttaccg gtgcggctgt
     1201 ggctttcact tcttctttgg atgcttccac cagtgcttca acctgcttca tatcgatgcg
     1261 gttatacagt gccttgaacg gattcacttt atggcccagc agcggttgct ggataccatc
     1321 ccaggtcagt tctgtattca ggaatgcttc tgcacgttca gtcagtttcg gcagtaccgg
     1381 cttcaggtac gtcatcagca cgcggaacag gttaatgccc atagagcaaa tcgcctgcag
     1441 atcggcatcg cgtccttcct gcttcgccac cacccacgga gcctgttcat cgacatagcg
     1501 gttagccagg tcagccaatg ccatgatttc acggatagct ttaccaaatt cgcggctttc
     1561 ccatgcttcg ccaatcactt cagcggcatc agtaaaggtt ttgtacagtt gcgggtcagc
     1621 cagttcgctt gccagcacgc cgtcaaaacg cttgttgata aagcctgcat tacgggaggc
     1681 caggttaacc actttgttca cgatatcggc attcacgcgc tgaacgaaat cctccaggtt
     1741 gagatcgata tcatcaatgc gcgaagagag tttcgcagtg tagtagtaac gcaggctgtc
     1801 ggcgtcaaaa tgattcagcc aggtgctggc tttaataaag gtgccgcgag acttggacat
     1861 cttcgcgccg ttcaccgtca catagccgtg aacaaacagg ttggtcggct tgcggaagtt
     1921 gctgccttcc agcatggcag gccagaacag gctgtggaag taaacaatat ctttaccgat
     1981 gaagtggtac agctcggcgg tggagtcttt cttccagtat tcatcgaagc tggtggtatc
     2041 gccgcgcttg tcgcacagat tcttgaagga acccatgtag ccaatcggcg cgtccagcca
     2101 gacgtagaaa tatttgcccg gcgcgttcgg aatttcaaaa ccgaagtaag gcgcatctcg
     2161 ggagatatcc cactgttgca ggccggattc aaaccattcc tgcattttgt tcgccacctg
     2221 ctcctgcaac gcgccgctgc gggtccatgc ctgcaacatt tcgctgaaag aaggcagatc
     2281 aaagaagaag tgttcggaat cacgcattac cggggtagcg ccagaaacca ctgatttcgg
     2341 ctcgatcagt tccgtcgggc tgtatgtcgc gccgcagact tcacagttat cgccgtattg
     2401 atccggcgct ttacatttcg ggcatgtgcc ttttacaaaa cggtccggca ggaacatgcc
     2461 tttttccggg tcgtacagct gagagatggt gcggttttta ataaaaccgt tctctttcag
     2521 gcgagtatag ataagctcgg acaactgacg gttctcttcg ctgtgcgtcg agtgatagtt
     2581 gtcatagctg atgttaaagc ctgcgaaatc agtctgatgt tcctgactca tttcgccaat
     2641 catctgttcc ggtgtgatac caagctgctg agctttcagc atgatcggtg taccgtgggc
     2701 atcgtcggca cagatgaaat taacctcgtg gccgcgcatt cgctggtaac gaacccagac
     2761 atcagcctgg atgtgctcca gcatatggcc gaggtggatt gagccgttag cgtacggcag
     2821 cgcgcacgtc accagaattt tcttcgcgac ttgggtcata gtaggcatta cttctttgta
     2881 gtgaaaaggg gcttgatagt aacaaaatgg ccttatgtct gccatgtgat aacagcattt
     2941 ctcataaatg attaaatgtc gtagctggag tacactacaa ggcgacaatt gcgcaaatta
     3001 aaataaagga gacgggatga gcgagtccaa atcgccggat gccctgagag caatggtagc
     3061 cggtacgctg gctaattttc agcacccaac cctgaaacac aacctgacta cgcttaaagc
     3121 gttacaccat gttgcgttga tggatgacac actgcatgtc gaactaatca tgcctttcgt
     3181 gtggaataag ccttttgaag acctgaaaga gcaatgtagt ggtgatctgc tccgtatcac
     3241 tggcgcaaag gctattgact ggaagctgtc gtacaacatt gccacgctta agcgcgtcaa
     3301 aaaccaacca ggcattaatg gcgttaagaa cattatcgcc gtcagctcag gcaagggcgg
     3361 cgtgggtaaa tcctccacgg cggtaaacct ggcactggcg ttggctgctg aaggtgcgaa
     3421 agttggtatt ttggatgccg atatctatgg tccatcaatt ccaaccatgc tgggcgcgga
     3481 aaaccaacgt ccaacctcac ctgacggtac tcacatggca cctatcatgt ctcatggcct
     3541 ggcaaccaac tctattggtt atctggtcac cgacgacaat gcaatggtgt ggcgtggacc
     3601 gatggccagc aaggcgctga tgcagatgtt gcaggaaact ctatggcctg atctggacta
     3661 tctcgttctc gatatgccgc cgggcactgg tgatattcag ttgacgctgg cgcagaacat
     3721 tcctgtaact ggtgcggttg tggtaactac gccgcaagac atcgcgctga tcgatgcgaa
     3781 gaaaggcatt gtgatgttcg aaaaagtcga agtgccggta ctgggtatcg tcgaaaacat
     3841 gagtgtgcat atttgcagta actgcggtca tcacgagcca atttttggca ccggtggcgc
     3901 acagaaactg gccgagaaat acaacacgca gttgttaggc caaatgccac ttcatatctc
     3961 tcttcgtgaa gatctggata acggaacacc aaccgttatt agtcgtccag atagcgaatt
     4021 tacggctatt tatcgtgacc tcgcagatcg cgttgctgcc caaatgtatt ggcaaggtga
     4081 agtaatacct ggcgaaatcg cattccgcgc agtgtgataa aaaggcggtg cattgcaccg
     4141 cctaatcagt tctcctacat cacgggacaa tcatcaaact cgccacttct tgcgtcgttg
     4201 ataatgtgag tgatcacacc aaacacggca ttgctgcccg tgcatccatc gtcatccttt
     4261 ggcaatgctt ccttcttacc ggttcttaaa tcctccagat gctgacgtgg gtacttccgg
     4321 tatcttttta cgcgatattc cccatccaga gcgcatacaa gcagagaacc atcaaccggg
     4381 gtaagtgagg aatcgaccac cagcaatgca ccctgtaata ttccctcacg atgatgacta
     4441 tcagctgccc tcatgaagta ggtcgctgaa ggatgtctaa ttatctgctg atcaagagaa
     4501 atacggctct cagcataatc cgccgcagga gaagggaagc ccatagcgtt ttcacctcaa
     4561 aatactgttt atccatacag tatacattaa agaagcacct ttggtgcaaa cgcgttatgt
     4621 acatcaacca ccgctgatga ctttgtgcgc tttgctacta ttcaccaccg ctggatcagc
     4681 gtaacctcgt tactaatcaa ttaataagga attagccatg cctgcccgta ttcctctcga
     4741 tcctgcattg cccaaaaatt ttgactgcac tcctaacgag aaacgctcca aagcccagct
     4801 ggacgcctgg tggaaccatc cctatggagt tacagaacct gacgggaaaa ttgttgttta
     4861 ttgtctgaat ggtggggcgt gggaccgtcc atccatgctt tgtttggcag ataactatga
     4921 tgaagcctgt gaacttgccg aaagacagca ggcaagctgg gtaaaaacac ggtctgaacc
     4981 gacattcatg ttttcaaaaa aaccgccatt tatactggca aggatgccgc agcgaccgga
     5041 tcatcaacaa gaaattgttg ctgaattttc ctcaagggat gagatgaatc tcttctcatt
     5101 aaggcaggaa gaaagggatc acgtcgaagt gtctccaact cttgatcaca accggatgaa
     5161 cctggccaac tcgcctggta cagcaaagaa ttagagatgt ctattgcccg acttgaaaac
     5221 gaaaaagccg ctatccaagc ccagcatgaa ggggtagtga ccgggtagct acgagaactg
     5281 ccaactattc ggaaaaacgt acgctaatta gcgtacgttt tcattccatt gggcttcaga
     5341 cgccttcacc gtcttgttct ttgctacgga tattcgtaat gcatgttttc atccggataa
     5401 gggttgtcaa acgcatttgc agaaacacgg atcgtcaaat tatccgataa tccttcgatc
     5461 atctccaaaa tctcaggcgg acaacttttg tcaaaggtca acacaaagtc atagatcaaa
     5521 ccgtcatcac tactgttttc ctgtgtgtcc cattccagca attcaagatc ttcgtctgtt
     5581 atgccaagct ccgctgccag tctgctctga gaaggtttgc catactttag ctccgggtca
     5641 accctgtcgt cattgacgca gcctccagct tcaagctcga aatcgtaaaa accgcgcttg
     5701 ccagccatgc ggggcaatat caccttcaca actatatcaa gggtgtcacc acagaacggc
     5761 ctatcgtcca gtgtctcgcc cttgtactgg gttttggcaa tagccaccaa tttgtcacca
     5821 cgatcctcga tttgcacatc gtcataataa gcatcgaaaa attccgcatt ggtttcagcc
     5881 atcaaaccgg aaagctggtc atctataaga gattggcaat cgggtagtaa gacttcgaca
     5941 atatagtttt caagtgtgtc atggatccag acatttccac cacatagccc ttcactggta
     6001 agcatgtctg atagaagcct ggcgaggtca attgactggg taaggtcatt tggcaaaccg
     6061 ttgagtttcg agcgtctgtc attcatcaac ggtatatcag gaatgaagat atatgcctct
     6121 tcgagctgaa ccagcgggta ggtcttctcg gccatgagtg ccgcatggct cttgtaaccg
     6181 aaaaatgctg caaccagctc tcttgcatgt gaagctttta gtttttcagt ggattgggaa
     6241 acatgatttt gacgtaggaa atctgcgcaa agttttgaga gatcggtact catagtagtg
     6301 atcctgtctt aacaatgctg gactttaaaa gtagtggtaa gtgtttaagt cactacgctt
     6361 gccagcatgg ttaataaaga tcaactgtca ttggcaggta gttcgacttg ctgtctttta
     6421 ggtcacttac agccttggca atcagctccg atacctaaga taccagaatg gccgatttta
     6481 gcaagacgaa taaatgatgc tttcagctag ccatgtccta gcatggccag attttgggtg
     6541 ttcgtattta tttcattgac cagcattata atcgttaatc tggttactcg gagacgggct
     6601 gcaacatcga gcgcagcgcg tgaaggtgga aggataaaag ttggcagtta tctcagctac
     6661 ccggtcactc tcccatgaag tagttctgaa ccggattaga gaaatgcaaa acgataacag
     6721 aggctaaatc gaggtagaaa acacattatt taaggccact aattgtggcc ttaaagggga
     6781 gagatgctta ttggctatgg ctaaaaatca aatgagacac ttcatgtggt tgtagctcgt
     6841 caagatccgg tgccacaaaa ccttttccga aagacttcac aagtgattca ccagccgcat
     6901 ccttgtcacc tattacacga aagtcatacg gcagcattgc aagttgacga cgtaatccag
     6961 ctgagaaagt cgaacctaac agcgcccatg aatttccgcc cgcgttgtgc actgctgcac
     7021 ttttaaaaat cgactcagtt atccatattg ggccatcttt taaaggaaga taggttcccc
     7081 aaagtaactg tctgccgtgt gatcgcgtga aataccgggc catcttcgga ttgctatgct
     7141 gcttaggagc gcctggtctg tatgtctgat acccagccaa catcccatca aacctgtata
     7201 gagggaatgt aacaactcct tcgctttcat cgatccactg tctatatggg tgtatatcat
     7261 ggcggtagtt acgagtcagc aaatgagctt ccagctttcc aaatttggtc ataacttcgc
     7321 atccctcata acacttccca tttatcacca tatttctcca tagctcttag ccagtaaaca
     7381 tggccactag gcatagtgat gtgaggctca ccaaactctt ctccatgtac atcaaagatg
     7441 tccccaacag aaaattgggg gatttctgtt ccgttttcac ctatatgtgt aattcgtata
     7501 cgtttcggtt gggattgtcc acctttgagc gagttgttca ctgctctggc aattttctcc
     7561 cgcaattgct gcgttccgtg atacgtaacc gcaatatccc tcaactcgtt caccagttct
     7621 cgaatctggt gatctttaag ctcattattt gggtgattgg taacaactct ggttaattca
     7681 cccttgtctt ttgctcttac gtatgcagct cttgtgcatt cgacccatgt attaccgcct
     7741 acattaactt cgtagattag gctggactta tctgtcaatt cggaacaaat acgggccgtg
     7801 gtctggcaac tagagcattc gcagttatcg cggaaaccgt ggtctactgg cttcggtttc
     7861 acatcatcaa ctttttctac cctatcggaa tttaaatact tcgaagcctt gctatgccaa
     7921 tcgctggttt taggatcttg tccatccatc aaacgccagg cttcagcgat taactcataa
     7981 ctagtgactg tagcttcctg cgcatcacca ataagtcgtg taatctctct ttcaagccga
     8041 ccgcctagct ctgttttagt gcaatgttct gaccaatcgc cagcctcaag cagcgcgaga
     8101 atgcttaaaa tatcgtccaa tagatcagtg gtattatcga cttccttctg aaactcaccg
     8161 gctactgatt tatgcatcat tcgtcatcct catccgagtc atcctcatca caggatgaga
     8221 gtagtgggtt agtcattcgc cctacttgag tggcgtagcc atgccgacag agattgcgca
     8281 gcacactgta tatttcgaac atctcggttc gttcatcgcc aacatcaaac tcagatgcta
     8341 tagcgtggca ttcagtcgcg agagccgata ttttctgaaa taactctgct ttattcactc
     8401 ttcgactcct ttacgaagct gttcagcaat acttacgcat atctctgcgc ctctaatcag
     8461 ccccggaacg ttcttgtttg gcccaacttc accatcaaca aaatcaatca tcgcgttacg
     8521 agccatatcc acgccttgcg cacgtacttc agccagaaaa gcatcggtgg ctggagtctc
     8581 cgttaattcg ctaacccaat tatctccgtg ttcgtcggca cacatctcat cgaatgctcg
     8641 ctcagattct ttcagggctg cattctccgc cgctaatgcg tctctctgcg cccgcaactt
     8701 ctcaatttca gcagccatgt agtaaccaac catagcgaag acggcaaaag agttgtctgc
     8761 ctcatcaggc gacactgagg ccattagcag accatcgtaa taatcgtgac tcccattggt
     8821 gatcgccacc gcataggagt cgtggttctc acgcttgtga atgagaacta cggggttttc
     8881 gattttgtta ctcatgtgtt tttctcttct ttgctgttac ataagcacta ccaagtgctg
     8941 gtttattttc acaaataaga aaaggtagaa aataatttat ttaaggccac aacttatggc
     9001 ctttatttat tcatgccgaa ggatttgtag cgtctaattg ccggataaaa tggggtgagg
     9061 tgcttgccag taggctccac cggtccatgt gaaccccatg cgaataagag tgtttattgc
     9121 ggctttggta gactccgatt cgacactggc ttttcgaaac tcttgaacct ctttcgccaa
     9181 cctgtatgta attgtgcagg ggacgattgc agcccagcct ttctcatgac tgttaatgac
     9241 catctggatg tgaccatcca gaataggttg attaggcatg ttattgaaaa cgtctgcctg
     9301 tttttgaatg tgaataaaaa gccttgccag ctccatttgc tcactacgag acaagggatt
     9361 atcaagaggg ctttttgcaa aagcggctat acgctgtgcg tcaatgccaa acatcagcgc
     9421 acctcaccat ctttatggct ggagttaact tccgcc
//
LOCUS       DAPQZD010000186         9271 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPQZD010000186 DAPQZD010000000
VERSION     DAPQZD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.188"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            24..1127
                     /locus_tag="RO454_004276"
     CDS             24..1127
                     /locus_tag="RO454_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDY0936016.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSSLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(1122..1508)
                     /locus_tag="RO454_004277"
     CDS             complement(1122..1508)
                     /locus_tag="RO454_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0936017.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            1760..1972
                     /locus_tag="RO454_004278"
     CDS             1760..1972
                     /locus_tag="RO454_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936018.1"
                     /translation="MNTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFELNNKRFTLLNTGSGSLAVRIC"
     gene            2071..4179
                     /locus_tag="RO454_004279"
     CDS             2071..4179
                     /locus_tag="RO454_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HDY0936019.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIKSES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSGASDNAASESFTTDHEKRKSTE
                     DGSSDIPTPSKMSLEEALEELDSMEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLTMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            4275..5510
                     /locus_tag="RO454_004280"
     CDS             4275..5510
                     /locus_tag="RO454_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0936020.1"
                     /translation="MTTTALQNEKNHSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEINMLINA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            5691..9209
                     /gene="dnaE"
                     /locus_tag="RO454_004281"
     CDS             5691..9209
                     /gene="dnaE"
                     /locus_tag="RO454_004281"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDY0936021.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESKGCMESLERGRNYSFTALIK
                     NEKGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLRFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEHGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATAPERLRDQAE
                     LMGNLVIDAVKASRSFEMNPKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFIREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKT"
BASE COUNT         2640 a         2068 c         2232 g         2331 t
ORIGIN      
        1 cggcgtaact cggtgattgt catatgaaaa atagctttga cagagcacgc gctgcggaga
       61 acacctcaaa agaggcgata gagtatctcg aaagagcatc tcaaatgcag gccgttatga
      121 tctcccaggt cagcaatgac atgagattct cggacgcatt catgttattc actcgcttat
      181 ctctgctgat aaccagacgt cggccagaga tcgctgttca ttgtattttg atacatgttt
      241 tgccgcacat tgccgatgta aaagtaagtg acattaatag gttcatggtg aaccaactgg
      301 tcaacccact aatactggat ggcaaaattg ttatgggccg ccgcgttttc tctctgatga
      361 agcagttcct tagctggtgc gccttccagg ggatgataga cgtgtcaccg ttaaacgata
      421 tgtcacttaa caaagttgcc ggtggcgcaa agcccacacc tcgcgagcgg aagctgaccg
      481 acgcagaggt atgggtgttc tggaatatat gggactactt caatgtgtgt gctggtacaa
      541 aatgggcggc caggctatgt cttgtatccg caagacgacc tgacgaagta ctgcgggcta
      601 aaaaaagtga gttcaatctt aagcgtgggg tttggaatca aggcaagagg aacaaatctg
      661 cccgtgagca ttctctgcct ttaagctcat taatgcgcac ttgtattgaa gagttgttcg
      721 aatatggtaa agacagccag tggctcgtgc cttcgaataa aaaaatcggg aaagaccttc
      781 ctatgtctaa agtggcaata gcccaggcat tacgtcgtat tctggaacga ccagaactga
      841 tggagcttga gccatttaca ccccgagatt tgcgccgtac tgcgcgtagt tacttcccag
      901 cattaggcat aagccaggag gtatcacgta aaatcatgaa ccacagtctt gaggggatag
      961 atcgggtcta cgaccggcac gattatatgg acgagatgcg agacgcctta gaaagtttct
     1021 cgacgtacat cgcatcaatc gtagagcaac cggatttaga cgaaattgac cacaaattta
     1081 agggagatcg tctatcaaca gagcttattc gtgtaaattt ttcatagaga ctttatggcc
     1141 tcaacaacct tttgtgatgc gcctttctct ttaccgaatc gctcgttata tgcagcaaga
     1201 acctgttttt cgtcctcgtt aagaggagca gtgccttctt tgtataaaaa tgctgcgagt
     1261 tcgggttggc gttcttccag caccatcatc ataagacgac ttggctcaat gcccagtgcc
     1321 agcgccagcg gacgaacctt atcgataggc aaaggaattt tgccgctttt aattaaagaa
     1381 aggttgttgg cgtttttata cccaattgtt ttggctatct gggcctggct cataggtgag
     1441 gattcaatca accctgcgat aaaagcagcg tagcgacttt ctataaattc aatcttgtta
     1501 tcagacatgg ttacaacctt tgcgcgttca attctctctg gtaagtgctt accgatatta
     1561 catcaaaggt tagggttgta aagctattat catttttttc gataggcact taaaagaccg
     1621 gttaaaggcc attgtacgga gaaaaattag cccaaaacag gtaagaaaat caacttgcat
     1681 atgatatgaa tgtattcagt attgatacaa attttagtag tattccttac catagtataa
     1741 gttagaatgg attgattgaa tgaacaccac tatttccagc ctaatcgctc ttgagatcgg
     1801 acacgtacag aaattagctg atgagtgtgt agctgacatc ctcaccgatc taccgaatga
     1861 gcagattcag gttggtgtga atgacacaac tggctttata ttcgaactta acaacaaacg
     1921 cttcacgctt ctcaataccg gctccgggtc tttagccgtc agaatctgtt aacccctctt
     1981 ctccctgcgc gaatgactta gttccctgtt cgcgcagtgc tacattaaac acactagtaa
     2041 ataatttgtt ttcataacaa aggattagcc atgtctaaaa aacgttccat caaagaggtt
     2101 caggacttcc gtgacagtgt aaaacgagta gtcgctctcc tttcaggtaa aaacatccct
     2161 gttgcagaac gaggggacga cgcttatgta cgctataacg atgatggaga gccaattctc
     2221 gtaaacatcc catcaatccc ggataacgca acaccggcat tgatgaatgc tgtgcgcgga
     2281 tttctcgatc atgaggttgc tcacattttg tttaccgata ttcgtgtgtc caacaaaatg
     2341 agagaaaaag gacgcgttcc ttcctggtcg ctatggaatg ccttagaaga cgtgttcatc
     2401 gagcgaaaaa tgggtcaggt ctttaacgga acaagacgta atctgatggc aactcagcgc
     2461 cttataatcg aaaaagtctt taaaccaaag gcttcagagg ctattgctta ttgtggcaaa
     2521 gatcagcgcg cgctttttct aaacttcttt ctttgtccgg ttgtaagagc ctgggatggc
     2581 caagcaccgt tcgtagattt catggatgaa tattggcctg tcattgagaa accaatttca
     2641 ttattaaaag aacatggtat cgatgtggcc gtgcgtaaca tgtcttgcac cgaggattgt
     2701 gtaaaggtgg ctgcgaccat agctaagatc ctcaaagaca ctgaaagtga aagcaaaggt
     2761 aaggagtcag ctccgggaaa aacttccgat ccttcagacg ctgaccagac ggatgcctct
     2821 ggagaaaaca atgaagacaa cgaagatcat gagacaccct cagcgttaga taatcacaaa
     2881 tctatcaaat cagaatcaca cagtaagcac aaacatgata ataacgacag tgatgattca
     2941 gataattctg aatcatcaga aacaattttc gatgatacag aaaatgataa agaggtatca
     3001 gattctggtg cttctgataa cgcggcgtca gaatcattta ccactgacca cgaaaaaaga
     3061 aaatcgacag aagacggctc ttcagacatt ccaactccgt caaaaatgag tctggaagag
     3121 gctttagagg agttggatag catggaagat gaagtcggag gcatgacaga agatgcgcta
     3181 tccgaaacga ttaaaagcga gttaacagaa agctcgaaaa gcgaatacag gccatacaat
     3241 cgctcatacg acttcatcgg ctcgattgat caggcagaag cccatatcaa acggcttatt
     3301 aaaacattct ccgatattga tttaggtgga tatccaatca gccgttatcg catcgttcct
     3361 gaaggcaacc aactcttcga caaatatatt gaaaagcacc tttcgtcagg tgtttcgtcg
     3421 acgctggcaa aagacctgga acgtgcaata gcaagcagaa acagagttca gtttatacct
     3481 ggccagcgtc gggggcgcat tcatggttca agtatctaca gattaacaat gaatgatgat
     3541 cgcgtgtttc gtaaaaaaga agaatctaaa gccgttaacg cctgtgttca acaagtgatt
     3601 gatttatcag gttcaatgag tggtataacg atacaattgg ctcttgcaag tgcatatacc
     3661 atcgccgatg cccttgatcg aataaatgtt cccaacatta tcaccggctt cactacattt
     3721 ggtagtcata tggcggcagg agaacttaag gctgtcaagt atgagttctc tcgctttgaa
     3781 tctttaatgc tacctatcat caaaaattgg aatgaaaagg taaattctcg cgaagttcgc
     3841 tcacgtatgg ggtgcgtagg ctacacattc ccacttctta ataacgtgga tggtgaaagc
     3901 atagccagcc ttgcatcgtt attttccggt cgcatggagg acaggaagat catgcttgtt
     3961 ctgagtgatg gcgcgccgtg ggctgttggg agaggttttg acgctcattt gcgttcggtt
     4021 gcgaaacaaa ttgaaacgca gactgacatt gatttgatgg caattggcat catgactgac
     4081 gcaccggaga gattttactc aaatcatgcc ctggtaacga gcgttgatag tcttggttca
     4141 tctgtagtta ctgaactatc tcgtatcatt ttgaagtgaa taaaacagcc ttaatgataa
     4201 gtaaccactt acgatagtta atggtatatt tatataagaa gttgaacgct cattagagaa
     4261 caaaggaaaa acgcatgacg actactgcac tgcaaaatga aaaaaatcat tctgattacc
     4321 ttgtttgcaa gtggtgcggc aaatcatttc actattttaa gtcccatgta gccaatggta
     4381 attgcgaggg cattcctgag tcagtaaaag atgccgatcc tgacaccgta ctgaaaatgt
     4441 acacaacgca gtttccagat gagccaacgc tatcgaaaaa ggcacttgat gcaattcaag
     4501 ctaaacgtgc cgagcaaaaa agcgaaatgg ccaaatcttc tggcttgacc agtagccctg
     4561 gctacacagg cacagttgag tacaagacag atctggtcgc agctcacgaa ctgctaaacg
     4621 taacggtgga agaactcgga acaaaacgtg ggacgccgct catggttagc gtcaacgtca
     4681 atacgccgta tccagagttc gttccagaag tgaagaaggg ctacgtatat ggcgacttcg
     4741 aactgatcaa agatattttc atgatgcttg aacttggcat acctggctat ttgtggggtc
     4801 atgcaggaac aggcaaatcg tcattgccta cacagctatg tgctttgctc aatcgtccgt
     4861 tgatccgtgc ccaacataca gcatcaatgg aagaggcaca tgttacgggg caaattctgg
     4921 cgcgtgatgg ctctacgtat ttcgagcctg gcttgcttgc gctcgcaatg aagcatggct
     4981 gggtttacct cgcggatgaa tacgactttg cgtttccaca aattcttggc gtgtatcagc
     5041 cagtgctgga aggtgaggcg ttggtcatca aagaggcaac tccagaatgg cgtcgcatta
     5101 ctccgcatga acggtttgct ttcattggca ctggcaacac aaacggttct ggtgatgaaa
     5161 ccggcttgta ccagggtaca aacatccaga acgccgcgaa cttttcacgt tttggcatcg
     5221 tttcgaatgt gaaatacatg agcaaagagg cagagatcaa catgttgata aatgccggca
     5281 tcgtggatga atacgctgaa aagatggtta agtttgccgg tatcgttcgc gatggatatg
     5341 aagaacacct tatcagccag ccaattgggc ctcgtgagct tttgttgtcg gccaagattg
     5401 gaatgatgcg aggcgacttt gtgacaggta ttgagcgttc tttcattaac aaactccctt
     5461 cagcttctgc gcaagcggct cgtgaagttg ttcaaaaaat atttggttaa tcgtgcgtaa
     5521 aggatgtttc ggctctctta tcgctgcttc tgaaactggc aaggcttgtc tggggtgtcc
     5581 agacaagccc gattgtcacc aatcagcaaa agaagttgcg atttcgatgt atgggaagtt
     5641 cgtaggcttc ccaaatgaca aaatcaaaaa aaccagaaag gtaaaaacac atgaaggcac
     5701 tgatggttcg aactgacttc tcacttgggg agtcggcttt aaaagcagaa aacgcggtga
     5761 agattgccag agaagctggc tacaccgctg taatttcagc agatagcatg aatattgcga
     5821 gtgttattcc actacaacgt gccgctggtg acgacatggc ggttatttgt ggtgtgaaac
     5881 taaatatcgt tgatgatccc acatacgagc accgggctaa acttgctaaa gaatctaagg
     5941 gatgtatgga atcattagag cgaggacgta actactcgtt taccgctcta attaaaaatg
     6001 agaaaggata tcgcgacatc tgcgaactaa tgacggcggc caacacacga gaacagttct
     6061 actttgtacc tcgtctctcg ctcgaacagt tggtttctac atatgccaaa ggcaacatca
     6121 tcctgcttac ttccgacatc ggtagcgtgt tccaacgcaa cgattttgca aaaatcataa
     6181 gcacactgat tacagcgggt ggaaaagaca acttctatag tgtggtttat ccgcacccta
     6241 ccccattcta cgaccagatt aacgtccggg cgatgaaagt cgccagcgca ttgaaaatag
     6301 agccagtggc gttctatccc gcttattacg aatcgatcga cgatgcagac attaaagaca
     6361 ttgcgcacat ggttacaaac aacatcaaaa tcgaccagcc gcatcgtctg cgtatccccc
     6421 accagcgaga taacgccgtc aatggtcgcc gccatctcct tgaggcgctt aaagccttct
     6481 ccgttcgcat ggatgtgccg gtaacagctg caatggcctc aacaacgcag gataccatta
     6541 tcgatgcctg cacatggcgc tggcatgaat tgccgccagc actgccaaag atggcagacg
     6601 acgagcctgc aacgcttatg aaactggctg ttgcagggct gcgtaaacgt cttaccacaa
     6661 aagagtttgg ctacacacca cctgcttctg agaacagggt ttatgttgag cggctaaagt
     6721 acgaaatgga cacgctgact cgcctgagat tttgtggtta cttcctgatg gtacgcgatc
     6781 tgatgaatca cagccgtgaa actggcattc ctgtcgggcc tggtcgtggt tcctccgctg
     6841 gatctctggt ggcatggtgc ataggcataa ccaacgtcga cccaatccgt cacggtcttc
     6901 tgtttgaacg tttcatcaac cctgagcgtc tcgacttgcc ggatgcggat ttggacttca
     6961 gccaggcacg tcgccatgaa gtgatcgagt atctgaatga acgctacggc gaagattacg
     7021 ttgcaggcat tccgaacttc acctacctgg gcgcagcctc tgcactacgt gataccgctc
     7081 gtatttatgg tgtggagtcc gcagatatgg cggtatcaaa agaactgaag aacgtcgagg
     7141 atgatagcct tccattggaa gagctgcgcg aacaactggc aagtctcgac aaatacgcaa
     7201 caaaatatcc tgatgcattc aatgcagcct gcaagttaca aagccttatg cgtggctttg
     7261 gtagacatgc ggcagggatg atcgtagcag gtgttcctct gacagaacgt acaccggttg
     7321 agcgccgtgg tgacgcgcgt tgtatcgcat ttgacaagcg ttactgcgag gctatgggcc
     7381 taattaagct ggacgtgctt ggcctggcaa ctctcgattt gctcgatagt gcaaaacgct
     7441 acataaaaga gaacacaggt gaagatatca atcttgatgc catttctctt gaagatcgca
     7501 aggtgctgga tggttttgct gctgggtata ctcaaggtgt tttccagctt gaatcaggcc
     7561 caatgcgcaa gctgcttaaa gatttaggtg gtggaattga gccaatgagc tttaaaacgg
     7621 tcgtcgctac gactgcgctc ttccggccag ggccaattca gtcaggcatg ttggatgact
     7681 atgtttctgt agccaaaggc tttatgacgc cggaatcatt acaccccgtt cttgatgaac
     7741 ttaccgcaga aacaaatggc gtgattctct atcaggaaca gacgatgaac gcgactcgat
     7801 tgcttgctgg cttcacaatg gctgaagctg acggtgttcg taaagcgatc ggcaaaaagg
     7861 acatggaaaa aatgaagagc atgggcgaga agttcatcgt tcaggctcaa gctggctgga
     7921 tagacgttga gctggaagat ggcactacac agcgcattca ccgtgcggaa cattttaaat
     7981 gcgaagacgg aactctgaaa actgtcgaag aggcacttga gcacggcgca aaactaccta
     8041 taaacgcagt acgcgttaca gcgtcacatc cagggctatc agagatgaaa gcgaaggaga
     8101 tctggaccgc attcgaaaaa aatggtgcct accagttcaa caaatcacac tctgttgctt
     8161 actccttaat cagttatcaa tctatgtggt tgaaaactca ttatcccgca gagtttttcg
     8221 ctgctgctct cactattctt ggcgaagata aacaccaagg gctggttaaa gatgcgctga
     8281 cctatggtat tcgcgtattg ccaccagacg ttaacgtgtc atctaaccga atcgagatcc
     8341 gcacgcttga agatggcagc caggtgctgt atgcgccatt ctctgctgtg aaggggtgtt
     8401 ctgagaatgg ctgccaagcc atcatgagag cgcgagaaaa agttggcggc aaattcgagt
     8461 cactggcgca attcgaagaa gctgtcgaga aacgtgcctg taatagtcga gtgcgcgaat
     8521 cgctgcaaaa agtaggggcg tttgcatcca tcgagccagg tagtctgcca gcaactgctc
     8581 cagagcgcct acgcgaccag gctgaattga tgggaaatct tgtcatagac gcagttaaag
     8641 cctcacgttc gtttgagatg aaccccaaac gttcggctga aattaacgta ctcatgacgc
     8701 ggatggcggc tgaaatgggc ttaggtgatg aactaatccg ccccagcatt ggtattaagc
     8761 cgaaaatcat gatcattctg gacaatgcga acggcaatga tgctcgaacc ggttacttta
     8821 tggagaacgg atacgacgac tttaaggcca agctattgac ggctggagat ttacgcatgg
     8881 gcgatctcta tgtcacaggc gtttgcaaga aggttaaaga caaagaaaaa gactacacca
     8941 aagacgagat cggccagttc accgacttta tacgtgaaga gatcaatctg gtgcgtccga
     9001 cctatgtgct gacgtgtggc agccgggcaa cggcattgtt taacaacaaa aacaagccat
     9061 ccgatttggt cggcaggaag gagtatcttc cagaccttga cgttactgtc ttttacgggt
     9121 ttaaccccaa cattctttac ttcagacctg aagagggaga acgactagaa gcgatattag
     9181 ccgacgtagc agaaactatt aagacgtgat ccaataaaca tggccataaa ggccatgttt
     9241 tcccttatcc cctccccccc ctatcttcaa g
//
LOCUS       DAPQZD010000187         9239 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPQZD010000187 DAPQZD010000000
VERSION     DAPQZD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.189"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..824)
                     /locus_tag="RO454_004282"
     CDS             complement(<1..824)
                     /locus_tag="RO454_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDY0936022.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTIFLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSG"
     gene            complement(821..1849)
                     /locus_tag="RO454_004283"
     CDS             complement(821..1849)
                     /locus_tag="RO454_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDY0936023.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVTSKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1946..2761
                     /locus_tag="RO454_004284"
     CDS             1946..2761
                     /locus_tag="RO454_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0936024.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            3150..3293
                     /locus_tag="RO454_004285"
                     /pseudo
     CDS             3150..3293
                     /locus_tag="RO454_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3304..3819
                     /gene="ogt"
                     /locus_tag="RO454_004286"
     CDS             3304..3819
                     /gene="ogt"
                     /locus_tag="RO454_004286"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDY0936025.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            4014..4766
                     /gene="fnr"
                     /locus_tag="RO454_004287"
     CDS             4014..4766
                     /gene="fnr"
                     /locus_tag="RO454_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDY0936026.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4918..5868
                     /gene="uspE"
                     /locus_tag="RO454_004288"
     CDS             4918..5868
                     /gene="uspE"
                     /locus_tag="RO454_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDY0936027.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5918..6175)
                     /locus_tag="RO454_004289"
     CDS             complement(5918..6175)
                     /locus_tag="RO454_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDY0936028.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            6419..7450
                     /gene="ynaI"
                     /locus_tag="RO454_004290"
     CDS             6419..7450
                     /gene="ynaI"
                     /locus_tag="RO454_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDY0936029.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            complement(7501..9114)
                     /gene="mppA"
                     /locus_tag="RO454_004291"
     CDS             complement(7501..9114)
                     /gene="mppA"
                     /locus_tag="RO454_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDY0936030.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         2492 a         2196 c         2061 g         2490 t
ORIGIN      
        1 ccagaaaaag aaaaaaaaca tcccagtgtg aaatttttat ccttaaaagc atgaagatcc
       61 attatcggat tggatattgt aagtgtacgc gcagcaaata aaataaaact caccaatgcc
      121 agaactgatg tcagaaaaat agtattatca tccagccatc cccagcgcgc tccttcttca
      181 agggtatatt ccagacatcc cagtgtcatg gccaacagga taatactcgg ataatcagca
      241 actttcagta aagagagatc aggtttatca aaattaacca gaaaaggaat actcaaaact
      301 aaatatatgc caggtaatat attgatataa aatagccatc gccagtcgag gttatcagtg
      361 atccagcccc ccagagttgg ccctagcgtc ggtgacagtg aggccagcgc actgactacc
      421 gcagcagcca accccaactc tttaccctgg taataaataa acgccgtcgt gaaaactaaa
      481 gggatcatgg atgcgccagc agctccctgt aaagcacgaa aaaaaatcat gatctgaata
      541 ttccaagcaa gtccacacgc gatactcatt aaagtaaata ttcccgcgga tagagtaaaa
      601 agccaacgag tggaaaatac ccgtgacagc cacccagaga gcggaataac aataatctca
      661 gcaattaaat agctggtctg aagccaggcc atttcgtctt tacctgcgga cagaccacca
      721 cctatttcat ttagggatga cgatacaatt tgaatatcaa gctgagcaat aaataatccc
      781 atacacatac tggcgaaagc aatatgttta cgcatagaca tcatctctca tcaacactga
      841 cggtgacgga cagtcccggg cgaagaagtt gctttaattc ctttgcgtcg tcaaatgcaa
      901 tccgtaccgg gacacgctga acaattttgg tgaaattgcc agtggcattt tctatcggaa
      961 tcaagctgaa actggctcct gtcgctgggg aaaggctttc aatgtgaccg tgaaacactt
     1021 cccctttgag aatatcagca cggatctcag ccttcattcc tggcttcatt cccgctattt
     1081 ggttttcttt ataatttgca tcaacccaca actcactgac tggtactagt gaaactaacg
     1141 atgtgccgcc ttccacccaa ctgcctgtat gggcgctgcg gtttgcgaca atgccatcaa
     1201 tcggcgcacg aacaagggta tattccagat ccagtagtgc ctgagtcaaa gcggcctgaa
     1261 cttcttcgat acttgcacgt acgttttctt cctgagcaga taaaactgcc agccgttgct
     1321 tctccaccag gaaatcgttc gcggctttgc gctcctttgc tgcgacctgc tggtagtcaa
     1381 acctggcgtt atcaataatt tgttgcgata ttgccgctga ttgtgcgagt ttttcataac
     1441 gttcagtatc tcgcaggcgc ttttgctcct catgttttac tgcctgccat gtttcactgg
     1501 cactttgaat tatcgactgc tgcatcgcaa tagtggcttg aatgctttcg agattggctt
     1561 tgctactttt tatttttgcc tcaagcctgg cgacatttgc acgataatcc cgatcatcga
     1621 gacgcaggat aatatcgcct ttttttacac tctgattatc gcgcacctct atagcagaaa
     1681 tataaccaga aactttactt gtcaccgtgg tgatattacc accaatgtaa gcatcatccg
     1741 tcgtctgtat aaatcttcct ttgcttaccc accagataaa aaagacaacg ccagccaaaa
     1801 gaataccgat cgctaccacc ccaataagct gttttttaga aattatcatc actctttaca
     1861 ccgtatatat ccggaaactt gcatacgtca tatttaaaat ttaacatggc ttatttgatg
     1921 atttctgaca aaatacctac tgtaaatgac aatcacactg caacccaaag catcaccagg
     1981 gcatcacatt atcggactgg attcggaaca tctgaatgga ggtacagttc cctggcataa
     2041 acatctctat gcccagttgc tttacccggc tgaaggagtc gttcgtgtat gggctggaga
     2101 aagcgtctgg ctagtgcatg ccagcagcgc gctttggctg cccccacaaa tgccgcataa
     2161 gtttgttgcg acaggcaacg tacttttaaa aaccgtactg gtttcagaag cggaaagtga
     2221 aacgctggga aaagtttgct tcatgacggg tatttcaccc ctgttaaggg aattactaat
     2281 tgcaattaac caacttccac cgtcgcaaag taccacagat aaacaacagt tacgtttttc
     2341 agccctcgag acactgatac ttcaggaaat aaaaatgggg gtgaaaatgt cacttgaact
     2401 cccttggcca aatgatgagc ggctacagca gctttgtgag aatttgctaa acaaccaagg
     2461 gtatctgcca acactggata atctagcgga taaaattaac gtcagtagcc gtacactcat
     2521 gcgcttattt gttaaagaga cgggattaac atttcgccac tgggttcagc aaatgcacgt
     2581 tatttctgca gtgacgcttc ttgatgatgg atattcgttg accaaaattg cccatcgtct
     2641 gggctatgcc tcagctgaat cgttcggcaa tatgtttaag cgtcgaacgg gctactcgcc
     2701 cggtaaattt actcgtcgat taacgatgca tgattatgca atcacaagac aaatgattta
     2761 acttatattt tcgtgaaatc tgtcactgaa gaaaattggc aacggatggg taaaaccgtt
     2821 ataacactgt caccggtcac caggacccca ggccggattt aagacgagga tgcactgctg
     2881 tgtgtactgt agagtctggc ggatgtcgac aggctctatt tttttattca ttttttagcc
     2941 ctgtaaataa ccacattctc gccgggattg gggaatgagt gacgagatca gtggtgtctg
     3001 gcggctatca cgccacagat caaataacag ataacctacg gtaaaatttc gctgactgga
     3061 attatcccac cctcgatcca tgtgatggct tgccctcatt tcttcaggca gaaaaatcaa
     3121 aaaatcgctt aatactatca gactgcgaca aaccagacgc gaaactgggt acttactatt
     3181 cgttagtctt accctatcca cttatctttt tggtggtatg gctgctgatg ttgctggcgt
     3241 ggtatcttgt tggcttgccg ataggtccgg gtatttaccc acgtttgtct taagagagaa
     3301 cggatgctga gattacttga agaaaaaatt gccacgccac tgggtccact gtgggtgatt
     3361 tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag agtacagcga acgcatggtg
     3421 cagctgctgg acatccatta tcgcaaagaa ggctatgagc gcatttctgc caccaaccca
     3481 ggtggtttaa gcgacaagct gcgtgaatat tttgccggta atcttagcat tattgatacg
     3541 cttcccactg ccacgggggg gacgccattt cagcgcgaag tctggaaaac actgcgcact
     3601 atcccctgcg ggcaggtaat gcattatggc caactggctg aacaattagg ccgccctggc
     3661 gcggcgcgtg ccgttggtgc ggcaaacgga tcgaatccca tcagcatcgt cgtaccttgc
     3721 catcgggtta ttggccgaaa cggcaccatg accggatatg caggcggagt tcagcgaaaa
     3781 gagtggttat tgcgccacga aggttatctt ttgctgtaaa cattaaacaa tttgtgccag
     3841 cttgttcaca cttttatgta aagtcaccct taacaactta agggttttca aatagataga
     3901 catatattta catctaatat cggaattctc tgctgttaag gtttgcttag acttacttgc
     3961 tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg cttgagcaga cctatgatcc
     4021 cggaaaagcg aattatacgg cgcattcagt ctggcggttg tgctatccat tgccaggatt
     4081 gcagcatcag ccagctttgc atcccgttca cactcaacga acatgagctt gatcagcttg
     4141 ataatatcat tgagcggaag aagcctattc agaaaggcca gacgctgttt aaggctggag
     4201 atgaacttaa atcgctttat gccatccgct ccggtacgat taaaagttat accatcactg
     4261 agcaaggcga cgagcaaatc actggtttcc atttagcagg cgatctggtg ggatttgatg
     4321 ccatcggcag cggtcatcac ccgagtttcg cgcaggcgct ggaaacctcg atggtatgtg
     4381 aaatcccgtt cgaaacgctg gacgatttgt ctggtaaaat gccgaatctg cgtcagcaga
     4441 tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga catgatcctg ctgttgtcga
     4501 agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt cgttttgccc
     4561 aacgcggctt ctcccctcgt gaattccgcc tgacgatgac tcgtggtgat atcggtaact
     4621 atctgggcct gacggttgaa accatcagcc gtctgctggg tcgcttccag aaaagcggta
     4681 tgctggcagt caaaggtaaa tacatcacta tcgaaaataa cgatgcgctg gcccagcttg
     4741 ctggtcacac gcgtaacgtt gcctgattct tccgcataac tcactatcct tctgtcatat
     4801 cattaaattt ttctgattta ttgatctgac agaaggttca tcactgtttc attcaccaga
     4861 tatgggttaa tcttttaatt acaaactgcg ttgacagttg ttgtaaggag accctgtatg
     4921 gctatgtatc agaacatgct cgttgttatc gatcctaacc aggacgacca accagcattg
     4981 cggcgagctg tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc
     5041 tatgacttct catacgaaat gaccaccctg ctctctccgg acgaacgtac cgctatgcgt
     5101 cagggcgtca tcagccagcg tacagcctgg atccacgagc aggcaaaata ttatctcaat
     5161 gctggcgttc ccattgaaat taaagtggtc tggcataacc gtcctttcga agccatcatt
     5221 caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt
     5281 ctggaagcgg tgatttttac gccaacggac tggcatctgt tacgcaaatg cccaagcccc
     5341 gtgtggatgg tgaaagacca gccgtggccg gaagggggta aggcgctggt ggcggtgaat
     5401 ctcgccagtg aagagccgta ccataatgcg ctcaatgaaa aactggtcaa agagacgatc
     5461 gaactggcag aacaagtcaa ccataccgaa gttcatctgg ttggcgctta tccggtaacg
     5521 ccaattaata tcgcgattga actgccggaa tttgacccga gcgtttataa cgatgccatt
     5581 cgtgggcaac atttgctggc aatgaaagct ctgcgacaga aattcggcat taatgaaaac
     5641 atgacgcacg tagaaaaagg tctgccagaa gaggtgattc ctgatttggc ggagcactta
     5701 caggcgggga ttgtggttct gggcacggtc ggacgcaccg gtatttcagc agcattcctc
     5761 ggcaacacgg cggaacaggt gattgatcat cttcgctgcg acctgctggt cattaaacct
     5821 gaccagtatc agacacccgt tgaactggat gacgaagaag acgattaacg actcttcaat
     5881 aactgacaag gcccgagtta tcgggcctat tttttactta ctgctcttcg ccgccaagga
     5941 aataaattcc caacgggatt gccagcaaca cagtaaagac caggctataa acaaaaatca
     6001 tcgatgactg aatcacatac attgatgtcg tccactcaga cagcggaata ttgtactgtt
     6061 ctataacgcc gccgatggtc gcgcgagtca caaccgacgc cgcaataatg aaaaccgcca
     6121 acaaaccaaa gagaaatttc ttccctttcg ctgacttcag tttcgccata atcatgatgt
     6181 aatccttgtg cagacaaatt ttttgttacc taaacgtaac aataccatga catttcaccg
     6241 ttgtctgctc tgaaaacctc acctcttagc tggatttaca atcaacgcat tgattagtta
     6301 gctaatgttg ctacttcctt ccctgactat tgatgatctg aatgaattgt cgcggtaaaa
     6361 tagtcccgcc aggggtttcc ggcatgacga caattatctg gaggagcctg ataaaacaat
     6421 gatcgctgaa ctgtttacaa ataatgcgct taatctggtc attattttcg gtagctgcgc
     6481 agcattgatt ctgatgagct tttggtttcg ccgtggaaat cgtaaaagaa aaggattttt
     6541 attccatgcg gtgcaatttt taatctacac cataattatt agtgctgttg gtagcatcat
     6601 taactatgtt atagaaaact ataaactcaa atttatcacc ccaggcgtta tcgattttat
     6661 ctgtacgtcc ctgattgcgg ttattctgac gattaagcta tttctgctga ttaatcagtt
     6721 tgaaaaacag cagattaaaa aaggtcgcga tatcaccagt gcgcggatca tgtcgcgcat
     6781 tatcaaaatc accattattg tggtgcttgt tctgctttat ggcgaacatt tcggcatgag
     6841 cctttctggc ttgttgacct ttggtggtat tggtggtctg gctgtcggta tggccggtaa
     6901 agatattctg agtaacttct tttccgggat tatgctctat ttcgaccgtc ctttcagtat
     6961 tggcgactgg atccgttcac cggacagaaa tatcgaaggt acagtagcgg aaattggctg
     7021 gcgaattacc aaaattaaga cctttgataa tcgtccattg tacgtaccga actcgctgtt
     7081 ttcgtcgatc agcgtagaaa atcctggacg aatgaccaac cggcgcatta ccacgaccat
     7141 tggtttacgt tatgaggatg cggcaaaagt gggcgttatt gtcgaagccg tacgtgagat
     7201 gctgaaaaat cacccggcca tcgaccagcg acaaacctta ctggtttatt tcaaccagtt
     7261 tgctgattcg tcattaaata ttatggttta ttgctttacc aaaaccacgg tatgggctga
     7321 atggcttgct gcacagcaag acgtttattt gaagattatc gatattgtac agtcacacgg
     7381 cgcggacttt gccttcccca gccagacgct gtatatggat aacattacac cggcggacca
     7441 gggtcgctaa atcagacatc actggcgtta tccacaaggg taacgccagt tctcatcaca
     7501 tcaatgcttc acaatataca tagtccgact gtacgccaca tcttcagggt tgttaatggg
     7561 ataacctttc agccacggct tgattaatcg tccattggta tattgataaa ttggtgcaat
     7621 cggtgcttgc tccatgagga ttttttctgc cgcgttgtaa tcggcattac gcgctttaac
     7681 ggtattttcc gtcgatgcct gggccagaac tttgtcatat gccggattgt taaagcgtga
     7741 aatattcccg gaatgcgttg acgttaataa tgtcaggaaa gtggagggtt cattataatc
     7801 ccctacccac gaggcgcgga taacatcaaa attgccggta ttacggctat cgatataggt
     7861 tttccattcc tggttttgca atttaacatc tacgcccagg ttctttttcc acatcgatgc
     7921 tacagcaatc gcaatttttt gatggttttc tgaagtgtta tacaaaagcg tcagcttcag
     7981 cggtttttgc ggaccataac cagcggcgct caacaaagtt tttgcctgcg cattcagttc
     8041 ttcctgactc atttgttcaa acggcgaagg ttccggcgta aatcccgcgg taacatctgg
     8101 tgtaaaatgc cacgctggct tttcgcccgt ccccaacact ttttcggtca tcaggcggcg
     8161 atctatcgtc atacttaatg ccaggcgaac gcgctgatct gccgtcggcc ctttttgcgt
     8221 gttaaacgca taataatagg tcccgagctg cggcggcgta taaacctgcc ccggaatatc
     8281 cttcaacagc ttctgataca tatttttcgg gaaggattcg gtgatatcaa tatcccccgc
     8341 gagataacgc ttagtggctg cggattcctg attaattggc aggaaggtca ctttttgcag
     8401 taccgttttg gcgttatccc aataatgggt attcggcact acgaccagtt tttcattgac
     8461 tacgcgctct ttaagaacat aagcgccatt gccgatcaga tttccgggtt tcgtccactc
     8521 tttactgctt tctacgttgg ctttttgcac cgggaagaag gcaaagttag cggttaaatt
     8581 cacaaaccac ggcaacggtt tatcaagctg gattttcaaa gtatgggcat caactgcggt
     8641 aacgccaagc tggtcaggcg tggctttacc atcaataatc gcctgtgcgt tgttgattcc
     8701 cgccagcgcg gcaaaccatg caaatggcga aagcgttttc gggtccacca gacgttgcca
     8761 gctgtagaca aaatcttgcg ccgttaccgg tgtgccatcc gcccattttg cgttatcgcg
     8821 cagggtaaaa gtccagatac ggttgtcatt acttttccac tgagtcgcaa cgccggggac
     8881 aatctcccct ttctcgttct gattcaccag tccttcaaac agatcgcgaa tgacctgaat
     8941 ctccggcagg cccacggctt tagcgggatc cagcgacgct ggctcatctt taatgtggcg
     9001 caccagctcc tgcttctctg ccagtactgt gccgctcgga acttcagcag catacgaaag
     9061 agaaatgctg ctgaccaaca gcgcacaaca cgtgactgaa acagagtgct tcataagttt
     9121 ccctctaacc gacattaaaa ttgatgcgta attatttgtt tcgccttaaa gaaatgcaaa
     9181 caacttccat caaaaaagtg acaaaggaag catcaaatgt tttaagtatc aatatatca
//
LOCUS       DAPQZD010000188         9139 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPQZD010000188 DAPQZD010000000
VERSION     DAPQZD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.190"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..423)
                     /locus_tag="RO454_004292"
     CDS             complement(<1..423)
                     /locus_tag="RO454_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDY0936031.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAG"
     gene            681..1286
                     /locus_tag="RO454_004293"
     CDS             681..1286
                     /locus_tag="RO454_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDY0936032.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            1326..2189
                     /locus_tag="RO454_004294"
     CDS             1326..2189
                     /locus_tag="RO454_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDY0936033.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2179..3726
                     /gene="fdrA"
                     /locus_tag="RO454_004295"
     CDS             2179..3726
                     /gene="fdrA"
                     /locus_tag="RO454_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDY0936034.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3726..5144
                     /locus_tag="RO454_004296"
     CDS             3726..5144
                     /locus_tag="RO454_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDY0936035.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5297..6247
                     /locus_tag="RO454_004297"
     CDS             5297..6247
                     /locus_tag="RO454_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDY0936036.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQDGKEVIITTPECLPAALRGETGTHIIKT"
     gene            6257..7639
                     /locus_tag="RO454_004298"
     CDS             6257..7639
                     /locus_tag="RO454_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDY0936037.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            8016..9065
                     /gene="yahK"
                     /locus_tag="RO454_004299"
     CDS             8016..9065
                     /gene="yahK"
                     /locus_tag="RO454_004299"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDY0936038.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2293 a         2322 c         2548 g         1976 t
ORIGIN      
        1 acccgccaga aaacccagta atgtcaccgt ccagttcatc ccgctggcaa aaaatagcgt
       61 catgccgaga aagcctccgg gaataaaaga caataaaccg aaacgtcctt gccagaccac
      121 taccgctgag aaaggcacgg tagtaatcac gctcgcccag aacccagaaa tcccactcac
      181 cgaggccagc caaccggcag ccagcgcggc gaaaaatgcc cacaccatcc cggaataatt
      241 aaccgccaga cttctgataa accccgattt accgccgcct gcggcataaa aactgcaaaa
      301 ggcgacgaag ccaatggttc ctaataattc ccatccctga gagagcccga catgtgaaga
      361 cactaactgc caaagccctg cacaaatacc gacggtgaca cccgttgccg tgagtccgtt
      421 catcttgttc ccctctggtt agacatggac aaaagctact cttacatgtg cagactatag
      481 agaaataaaa cctgattgag tgaaagggta tcgggtcaaa gaaacaaata ttgcactacc
      541 gcacactgcg aaaagattgt tgaacatcat tcaacaaaaa ggcgaaatac ggcgaattaa
      601 atttaagcgg gttcttttat cagattatcg ttgctattct caattcatcg aaaacagcca
      661 caaaaaccag gggaatgtga atgactataa aaaatctacc tgccgactat ttattggctg
      721 cgcaacaggg tgatatcgat aaagtaaaaa cctgccttgc gctgggtgtc gatattaata
      781 cctgcgatcg tcaggggaaa acggcaatta cgctggcaag tttatatcag caatatgctt
      841 gcgttcaggc attaattgat gccggagcgg atattaataa gcaagatcat acctgtttaa
      901 atcctttttt aattagctgt ctgaacgatg atttaacgct actacgaatt attttaccgg
      961 ctaaacccga tcttaattgc gtaacccgtt ttggcggtgt cggcctgacg cctgcctgtg
     1021 aaaaaggcca tttaagtatt gtaaaagagc ttttggcaaa tacggagatt aacgttaacc
     1081 agactaacca tgttggctgg acaccgttgc tggaagcgat tgtgcttaat gatggtggga
     1141 ttaaacagca ggcgattgtc cagttattgc tggaacacgg tgccagcccg catctgaccg
     1201 ataaatatgg caaaacgccg ctgaaactgg cgcgggaacg gggctttgaa gagattgcgc
     1261 agttgctgat cgccgcaggt gcataaaccg ggaggcttgt tatcaacaca ccagaaaaac
     1321 ggtgtgtgtg ggcgctaact gcggatgctg attttctggc gcagcggggg caaggacagg
     1381 ttgaacaggt ctttgccaga gcggtaaata tcgcactccc ggctcgccag cagttgctga
     1441 cgctgctttg tgaagagtac gacaatgcgc ctaacagttg tcggttggca ctcactcact
     1501 ttaatggtct gttccggcat gatgataagg ttcagtttga cgatcaaggc attacgattg
     1561 gtcaacatca tcatatagag atgagtcatt gccagcgttg gctgtcccca accttgcaaa
     1621 tgaccgctgt gaattttcac cttatcgcct ggcaacagtg gtacgacatt attcatcagc
     1681 acctggggga aaatgaaacc ctgtttaatt atcgcggcga taatccgttt tatcaggcgt
     1741 taaataaaga actacatatt aaacgacggg cagttattca ggccgtaaac gataaacaaa
     1801 atatcgcctc agcagtcgcc agtatgatgg ggttagggat tggccttacg ccatcagccg
     1861 atgattatct gaccggactg gtacttattt tatttatttc cggacatccg gcggaaaaat
     1921 acaaagagga attttatctc ggcctgcaac gcggcagaaa taataccacg ttattaagtg
     1981 ccatcacgct ggaagccgca ttacaacaac gctgccggga aaatattcat cgttttattc
     2041 acaacattat ttatgacatc cctgggaacg caactcaggc aatagaaaaa attaaacata
     2101 ttggctccag ttccggctgc gacatgctgt atggcatggc cgatggttgt gcgctgagtc
     2161 aaacctacgg agggaattat gtcagttaaa atagtcatta aaccgaatac ctattttgat
     2221 tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac tcgacggcgt cgagcaggca
     2281 tttgtggcga tggcgaccga aatgaacaaa ggcgtgttga agaatttagg actgctgacg
     2341 ccggagctgg agcaggcgaa aaacggcgac ctgatgattg tcatcaatgg taaatcgggt
     2401 gtggacaacg agcagttgct ggcggagatt gaagaactgt tcaacaccaa agcgcaaagc
     2461 ggctcgcacg aggcgcgtta cggcactatt gccagcgcca aaaagcatat cccggaaagt
     2521 aacctggcag tgatttcggt caacggtctg tttgccgctc gcgaagcgcg tcaggcgctg
     2581 caaaatgacc tcaacgtgat gctgttttcc gataatgtct cagttgaaga tgaactggcg
     2641 ctcaaacagt tggcccatga aaaagggctg ctgatgatgg ggccagactg cggcacggcg
     2701 attatcaacg gcgcggcgct ctgctttggt aacgccgtgc gtcgcggcaa catcggtatt
     2761 gttggcgcat ctggcaccgg cagccaggaa ttgagcgtcc gtatccatga atttggtggc
     2821 ggcgtttcac aactgatcgg caccggcggg cgcgacctga gcgagaaaat cggcggactg
     2881 atgatgctcg atgccatcag catgctggaa aacgatccgc aaactgaaat cattgtgctt
     2941 atctccaaac cgcctgcgcc tgcggtggcc cgcaaagtgc tggaacgcgc acgcgcctgc
     3001 cgcaagccag tggtggcctg cttcctcggt cgtggcgaaa cgccagtgga tgagcagggg
     3061 ctacagtttg ctcgcggcag caaagaggcg gcattgaaag cggtgatgct ctccggcgtg
     3121 aaacaagaac atctcgatct gcatacgctt gaccagccgt tgattgcgga tgtgcgcgcg
     3181 cgtctgcaac cgcagcagaa atacattcgt ggtctgttct gcggcggtac gctgtgtgac
     3241 gaaaccctgt tcgcggtgat ggaaaaacat ggcgatgtct acagcaatat ccagcccgat
     3301 cctgaattcc gcctgcaaga tatcaaccgc agcatcaaac acaccttcct cgactttggc
     3361 gatgatgact tcaccaacgg caagccgcat ccaatgattg acccgaccaa ccgtatcagc
     3421 cgcttgatcg aagaagcgcg cgatccagaa gtggcggtga tcgtgatgga ttttgtgctc
     3481 ggatttggat cgcatgaaga tccggtcggt tccaccatcg aggcgatcaa agaagcgaaa
     3541 gcgatcgccg ctgccgaagg acgcgagttg atcattctcg cctatgtgct gggcaccgat
     3601 ctcgatacgc catcattaga acaacaaagc cagatgctgc ttgatgccgg agtgattctg
     3661 gcgagcagca gcaccaatac cggattgctg gcgcgtgaat ttatctgcaa aggggaggaa
     3721 gcctgatgag ccagtcactg tttagccaac cgttgaacgt tattaacgtc ggtatcgcca
     3781 tgtttagcga tgatctgaaa aagcagcatg tagaagtgac ccaactcgac tggacgccgc
     3841 cggggcaggg caatatgcag gtggtgcagg cactggacaa cattgccgat tcgccgctgg
     3901 cggacaaaat caccgccgct aaccagcagg cgctggagcg cattatccag tcgcatccag
     3961 tgctgattgg ttttgatcag gcgattaacg tggtgccggg catgacggcg aaaaccattc
     4021 ttcacgccgg gccgccggtc acctgggaaa aaatgtgcgg cgcgatgaaa ggcgcggtca
     4081 ccggagcgct ggtgtttgaa ggactggcga aagatctcga cgaggcggct gagctggcgg
     4141 cttccgggga gatcaccttc tcgccgtgcc acgagcacga ctgtgtggga tcgatggcgg
     4201 gcgtcacctc ggcctcgatg tttatgcaca tcgtgaaaaa caaaacctat ggcaacatcg
     4261 cttataccaa catgagcgag cagatggcga agattttgcg tatgggcgct aacgaccaga
     4321 gcgtgatcga ccgcctcaac tggatgcgcg atgtgcaggg gccaatgctg cgtgacgcga
     4381 tgaaaattat cggcgagatc gatctgcgct taatgctggc gcaggcgctg catatgggcg
     4441 atgaatgcca taaccgcaat aacgccggga cgacgctgct gattcaggcg ctgacgccgg
     4501 ggattattca ggcgggttat tccgttgagc aacagcgcga agtgtttgag tttgtcgcca
     4561 gcagcgatta cttctccggc ccaacgtgga tggcgatgtg taaagcggcg atggatgcgg
     4621 cgcacggcat tgaatacagc accgtggtga ccaccatggc gcgtaacggc gtcgagttcg
     4681 gcctgcgggt cagcggcctg ccggggcaat ggtttaccgg cccggcgcag caggtcatcg
     4741 gcccgatgtt tgccggttat aagccggaag attcggggct ggatatcggc gacagcgcca
     4801 tcaccgaaac ctacggtatc ggcggatttg cgatggcgac cgcgcccgcg atcgttgcgc
     4861 tggtgggcgg cacggtggaa gaggctatcg atttctcccg tcagatgcgt gaaatcaccc
     4921 ttggcgaaaa ccccaacgtc accattccgc tgctcggttt tatgggcgtg ccgtcggcaa
     4981 tcgacatcac ccgcgtgggt agcagcggca ttctgccggt gatcaacacc gccattgccc
     5041 ataaagatgc gggcgtcggc atgattggcg cgggcattgt gcatccaccc ttcgcctgct
     5101 tcgagaaagc cattcttggc tggtgcgaac gttacggcgt ctgacctgca aagtacgcgt
     5161 tgtctctgcc ggatgcggcg tgaacgcctt atccggccta cgagtggcac gagaattgta
     5221 ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt cataacccca tcaccctctg
     5281 tcgcggacat aacaacatga aagaacttgt ggtcgttgcc attggtggca acagcattat
     5341 caaagataac gccagccagt cgattgagca tcaggcggag gcggtgaaag ccgtggccga
     5401 tacggtgctg gaaatgctgg cttccgatta cgacattgtg ctgacccacg gcaacgggcc
     5461 gcaggtcggg ctggatttac gccgtgcgga gattgcccac gagcgcgaag ggctgccctt
     5521 aacgccgctg gcgaactgtg tggcggatac gcaaggtggc attggctatc tgatccagca
     5581 ggcgctgaac aaccgactgg cgcgtcacgg cgagaaaaaa gccgtcactg tggtgactca
     5641 ggtggaagtg gataaaaacg atccgggttt tgcccatccc accaagccca tcggcgcatt
     5701 ctttagtgaa agccagcgtg acgaactaca aaaggcaaac cctgactggc gttttgttga
     5761 agatgccgga cggggctatc gccgcgtggt cgcctcgccg gaaccgaaac gtattgtcga
     5821 agcacctgcc attaaggcgc tgatccaaca aggctttgtg gtgattggcg cgggcggcgg
     5881 tggaattcct gtggtgcgca ccgaagcggg agattaccaa agcgtggacg cggttatcga
     5941 caaagatctc tccaccgcgc tactggcccg cgaaattcac gccgacattc tggtgatcac
     6001 caccggcgtc gaaaaagtct gtatccactt tggcaagccg cagcaacagg cgctcgatcg
     6061 cgtagatatt gccaccatga cccgctatat gcaggaaggg catttcccac ctggcagcat
     6121 gttgccaaaa atcatcgcca gcctgacgtt cctggaacag gacggcaaag aagtgattat
     6181 caccacgccg gaatgcctgc ctgcggcgct acgcggcgaa acgggcaccc atattattaa
     6241 aacctaagga cgtaagatga aagaaaacaa tagccgccgt gaatttctga gccagagcgg
     6301 taagatggtc accgccgccg cgctgtttgg tacctcagtg ccgctcgccc atgcgggggt
     6361 agctggcacc ctaaactgcg aagcgaacaa caccatgaaa atcactgacc cgcattacta
     6421 tctcgataac gtgctgctgg aaaccggttt tgactacgaa aatggcgtgg cggtacagac
     6481 ccgcacggcg cgccagaccg tggagattca ggacggtaaa attgttgccc tgcgcgagaa
     6541 caagcagcat ccggacgcca cgctgccgca ctatgacgct ggcggtaagc tgatgctgcc
     6601 caccacccgc gatatgcata ttcatctcga caaaaccttc tacggcgggc cgtggcgctc
     6661 gctcaatcgt ccggcaggca ccactatcca ggacatgatc aaactcgagc agaaaatgct
     6721 gccggaactg caaccgtaca cgcaggaacg ggcggaaaaa ctgatcgatt tattgcagtc
     6781 gaaaggcacc actattgccc gcagccactg caatatcgaa ccggtttccg gcctgaaaaa
     6841 tctgcaaaat ttgcaggcgg tgctggcgcg acgtcagacg ggcttcgagt gtgagatcgt
     6901 cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa tccttaatgc gcgaagcgat
     6961 gcaggcgggg gcgcattacg tcggcgggct ggacccgacc agtgttgatg gcgcgatgga
     7021 aaaatccctc gacaccatgt tccagattgc gctggactat gacaaaggcg tcgatattca
     7081 cctgcacgaa accacgccgg caggcgtggc agccatcaat tatatggttg aaacagtgga
     7141 gaaaacgcca cagctgaagg gcaagctgac catcagccac gcctttgcgc tggcaacgct
     7201 caacgaacaa caggtagatg aactggcgca ccggatggcg gcgcagcgaa tttcgatcgc
     7261 ttcgacggtg ccgattggca cgctgcatat gccgctcaaa cagctgcacg acaaaggcgt
     7321 aaaagtgatg accggcaccg acagcgttat cgaccactgg tcgccttacg gcctgggcga
     7381 catgctggaa aaagccaatc tgtacgcgca gctctatatt cgtcctaacg aacagaacct
     7441 ctcccgctct ctgtttttag ccactggcga tgtattgccg ctcaacgaaa aaggcgagcg
     7501 cgtgtggccc aaagcgcagg atgatgccag ttttgtgctg gtggacgcct cctgttccgc
     7561 cgaggcggtg gcgcgtattt cgccgagaac ggcgacgttc cataaagggc aactggtgtg
     7621 ggggagtgtg gcaggttgat acgaacttga tatgtttgca acggtgaaca gtaagagatt
     7681 taagccccag ggagtggggc ggcctgagta tgagtgtgtc ggcagaaatg gacattatct
     7741 ttgaagaata tgcccttatt gacgttgtgt agataactat ttgtctacgt gaaaatatca
     7801 tcagttttgc cgatttttgt cttttacgga tagcaaatat cacacttata ggcgctcgcc
     7861 ttatggggaa cgttgctgct ctgtttcatt caggctcgcg gcatgttagg gctggatcgg
     7921 gtggtttaaa tattggctat attcaatgga cgcgttttgc cgcgatgaca tatcaggcat
     7981 cgtcaaatac acatagctaa tcaggagtaa acacaatgaa gatcaaagct gttggtgcat
     8041 attccgctaa acaaccgctt gaaccgatgg atatcacccg gcgtgaaccg ggaccgcatg
     8101 atgtcaaaat cgaaatcgct tactgtggcg tctgccattc cgatctccac caggtccgtt
     8161 cggagtgggc ggggacggtt tacccctgcg tgccgggtca tgaaattgtg gggcgcgtgg
     8221 tagccgttgg tgatcaggta gaaaaacatg cgccgggcga tctggtcggt gtcggctgca
     8281 ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg gctggaaaac tactgtgatc
     8341 acatgaccgg aacctataac tcgccgacac cggatgaacc gggccatact ctgggaggct
     8401 actcacaaca gatcgtcgtt catgagcgat atgttctgcg tattcgtcac ccgcaagagc
     8461 agctggcggc ggtggctcct ttattgtgtg caggaatcac cacgtactcg ccgctacgcc
     8521 actggcaggc cgggccgggt aaaaaagtgg gcgtggtcgg catcggcggt ctgggacata
     8581 tggggattaa actggcccac gcgatggggg cgcatgtggt ggcatttacc acttctgagt
     8641 caaaacgcga agcggcaaaa gccctggggg ccgatgaagt tgttaactca cgcaatgccg
     8701 atgagatggt ggctcatgtg aagagtttcg atttcatttt gaatacagta gctgcgccac
     8761 ataatctcga cgattttacc accttgctga agcgcgatgg caccataacg ctggttggtg
     8821 cgcctgcgac accgcataaa tcgccggaag ttttcaacct gatcatgaaa cgccgcgcga
     8881 tagctggttc tatgattggc ggcattccag aaactcagga gatgctcgat ttttgcgccg
     8941 aacatggcat cgtggctgat atagagatga ttcgggccga tcaaattaat gaagcctatg
     9001 agcgaatgct gcgtggtgat gtgaaatatc gttttgttat cgataatcgc acactaacag
     9061 actgaaaaaa attaataaat atcctgtggt ttaacatatt aatttcgctc tccttaactt
     9121 tttagttaag gagagcgaa
//
LOCUS       DAPQZD010000189         9037 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPQZD010000189 DAPQZD010000000
VERSION     DAPQZD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.191"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            181..1005
                     /gene="gadX"
                     /locus_tag="RO454_004300"
     CDS             181..1005
                     /gene="gadX"
                     /locus_tag="RO454_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDY0936039.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1374..2102
                     /gene="gadW"
                     /locus_tag="RO454_004301"
     CDS             1374..2102
                     /gene="gadW"
                     /locus_tag="RO454_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDY0936040.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2465..5578)
                     /locus_tag="RO454_004302"
     CDS             complement(2465..5578)
                     /locus_tag="RO454_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDY0936041.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5603..6760)
                     /gene="mdtE"
                     /locus_tag="RO454_004303"
     CDS             complement(5603..6760)
                     /gene="mdtE"
                     /locus_tag="RO454_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDY0936042.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6820..7149
                     /locus_tag="RO454_004304"
     CDS             6820..7149
                     /locus_tag="RO454_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936043.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7099..7626)
                     /gene="gadE"
                     /locus_tag="RO454_004305"
     CDS             complement(7099..7626)
                     /gene="gadE"
                     /locus_tag="RO454_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDY0936044.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8425..8997)
                     /gene="hdeD"
                     /locus_tag="RO454_004306"
     CDS             complement(8425..8997)
                     /gene="hdeD"
                     /locus_tag="RO454_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDY0936045.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2417 a         2098 c         2086 g         2436 t
ORIGIN      
        1 cctctgcgtt cttaagtccc ctttttatat aataaatcca gtcatccagc ccgagtatca
       61 tcgctcatta tcatcctgtt ttcccgcttc gtttaaattt atttatcaat caatttgact
      121 taagagggcg gcgtgctaca ttaataaaca gtaatatgtt tatgtaatat taagtcaact
      181 atgcaaccac tacatggtaa ttgtctaatt gcgtacgcaa gacataaata tattctcacc
      241 atggttaatg gtgaatatcg ctattttaat ggcggtgatc tggtttttgc ggatgcaagc
      301 caaattcaag tagataagtg tgttgagaat ttcgtattag tttcaagaga cacgctttca
      361 ttatttctcc cgatgctcaa ggaggaggca ttaaaacttc atgtacataa aaaagttcct
      421 tcattactcg ttcatcactg taccagagat attcctgttt ttcaggaagt tgcgcaacta
      481 tcgcagaata agaatcttcg ctatgcagaa atgctacgta aaagggcatt aatctttgcg
      541 ttgttatctg tttttcttga ggatacgcag tttataccgt tacttctgaa cgtgttgcaa
      601 ccgaatatgc gaacacgagt ttgtacggtc atcaataata atatcgccca tgagtggaca
      661 ttagcccgaa ttgctagcga attgttgatg agtccaagcc tgttaaagaa aaaattgcgc
      721 gaagaaggta cgtcatattc acagttgctt actgagtgta ggatgcagcg tgctttacaa
      781 cttattgtta tatatggggt ttcaattaag cgagttgcag tatcctgtgg atatcacagc
      841 gtgtcgtatt tcatttacgt ttttcgaaat tattatggga tgacgcccac agagtatcag
      901 gagcgatcgg cgcaggaatt accgaactgt ggctcggcgg caagtatggc tgcgcaaggg
      961 attttttacg gcactgaccg ttctgcggaa ggaataagat tatagagttt tactcagaca
     1021 taaaaaaaac ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa
     1081 accgttataa cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa
     1141 cttttactgg aaataagatc agccattttt ttataaacat aagctatacg ctgtgtgaaa
     1201 atataatcgt gttgcattta cttattatca attaactgtt atgcaaaact actttgtgga
     1261 taaaatttgg tcctaccaaa tatggcagtt tttgcacaca gaaacagtct ggcgtcattt
     1321 cattagtata ctgacattga aataatcgca gtaatgaaat ataagggata gtcatggctc
     1381 atgtctgctc ggtgatcctc gttcgtcgtt cattcgatat tcatcatgaa cagcaaaaaa
     1441 tatcgttgca taacgagagt atcctgctgc tggataaaaa tttggcagac gattttgcgt
     1501 tttgttcact ggatacgcga cggctggata tcgaagagct gacagtttgc cattacttac
     1561 aaaatattcg tcagttgcca cgcaatttag gattgcatag caaagaccgt ttgttaatta
     1621 accagtcacc ccccatacag ctggtgacgg cgatttttga tagtttcaat gacccccggg
     1681 tcaattcgcc gatactgagc aaaatgctct atctttcctg tttatcaatg ttttctcata
     1741 agaaagaact gatcccctta cttttcaata gtatcagtac tgtttcagga aaagttgaac
     1801 gccttattag ctttgatatc gctaaacgtt ggtatctacg cgatatcgca gaaagaatgt
     1861 acaccagcga gagtctcatc aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa
     1921 tattactcgc ctccaggatg tcgatggcca gacgattact cgagttacgt caaatacctc
     1981 tgcatactat tgcggaaaaa tgtggctata gcagtacgtc atactttata aatacattta
     2041 gacaatatta tggtgtaacg ccacatcagt tttcgcaaca ttcgccgggt accttttcct
     2101 gacatatttc gcatttgaat attggtcagg atctcacgtc tgcttcatgt gaaactcttt
     2161 cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatag
     2221 gtattttcag gaggaaaaga ctcggcatgt ttgggattat taagctaaca attcatacga
     2281 ttacgggaat gtgggtcagt attgtgctgt tcaaattgat gacgaatggc tggagtggtt
     2341 tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg
     2401 gtgaatggtt agcaggaaag agtaaggctg aaccctcacg ttcaacctta ctctcattta
     2461 cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa
     2521 cgaagtaaat tgccagcact gttgcggcaa acatcccgcc cattacgccg gtacctaccg
     2581 cgttttgcgc gccagagccg gcaccatgac tgataaccag cggcagcacg ccgagaataa
     2641 aggccagaga agtcatcagg attgggcgta aacgcatacg cgctgcttcg atgattgcct
     2701 ctaccggtgt tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg
     2761 cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtaa acgtcattac
     2821 ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga acaaccaaca
     2881 tcaccgagaa cggaattgac cagctttcat aaagtgcggc gagggcgagg aacaccacga
     2941 ccagtgaaat ggcatatagc gcgggagcct gatttgagga taacgcttcc tgatacgaca
     3001 gtccggtcca tgagtagccg acgcctgccg gaagttttgc gaccaggtct gccataaatt
     3061 tcatggcgtc accggtactt ttcccggcag ccgcttcacc taaaatctcc attgacggga
     3121 tgccgttgta gcgttccagt cgcggtgaac cataggtcca ttcggtagac gaataggcag
     3181 aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca
     3241 acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga
     3301 agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg
     3361 ccacacccat agcctcggct ttcgcagcgt tgacgttcac tttgaacatc ggcgtatctt
     3421 ccaggccgtt cgggcgtacc ccgatgacct gattcggtga ttgcgctgcc agtgataaca
     3481 gctcgtttcg cgcctgggtc agtttttcgt gccccagatt gccgttgtcc agcagttcca
     3541 tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga
     3601 cggctttatt gatactgctt agcgcaatca ttgcccgctg aataatcgcg gtaaccgagt
     3661 tttcctcgcc gacacgttca gaccacggct tgaggctgat aaacgccaga ccgttgtttt
     3721 gcccctgacc actgaagcca aagccaccaa cggtaaacac cgactgaaca ttatttttct
     3781 cttttgtcag ataataatcc gtcacctgtt gtagcacttt cgtggtgtta accatggttg
     3841 caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag
     3901 aggtcggcgt acgcaggaac agcaccgcca tcccggcgca aatcagtagg tagaccacca
     3961 tgtagcgacc ggtacaacgc aacagcgagc gggtgctgtc ggtatagtgc tgagttgatt
     4021 tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg ccctccggcg
     4081 cggctttcag aatggtggcg cacagggcag gggtcaggct cattgctaca aatactgaaa
     4141 gcagcatgga ggagatcagc gtgatggaga actggcggta gatctccccg gtcgcaccgc
     4201 tcataaaggc catcggcata aacactgcgg aaagaacaac ggcaatcccg accagcgcac
     4261 gttggatctg ccccatcgat ttatgcgtcg cttccttcgg cggtagctta tcttccgcaa
     4321 tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac cctatcgcca
     4381 gcaccatccc gaacatagtt aacgtgttga tggtgaaacc aactgccgac aagatcgcaa
     4441 acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggcg cggaaattct
     4501 gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagc gttttgaaaa
     4561 cttcctgaat agaaattttg ataaacggtg tggtgtcgta aggataaacc gtcttcaggc
     4621 ttgcagggaa ataagccgat aaacggttca gctcctcttt caccgctcgc gaggtatcca
     4681 gggcgtttgc tccggtagcc agtttgatgg cgatcccggc ggcaggtttg ccgttatagc
     4741 gcgccacggt ggaataatct tccgccccaa gttcgacgcg ggcgacatca cgcagcagca
     4801 cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca
     4861 gacgcgtctg cacaatgatc gaggcgttca gttgctggtc tgccgcctgt ggcatgccac
     4921 ccagttgacc accagaaatc tggttgtttt gcaccttaat ctgggaaata acatcggaag
     4981 gtaccaggtt atatttattg agtttttgcg ggtccagcca gatacgcatg gcgtactcgg
     5041 aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag
     5101 acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaatgccg
     5161 ctaccatcag gatattactg ctcgacttat cgacgctaat cccctgctgc tgcaccgctt
     5221 caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt gcgatatcag
     5281 gcgatgtccc cgtctcgaag gtcagagtga tggaggcatt acctgccgca tcgctggttg
     5341 aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg
     5401 agtcttctac cgtttgcgca tcggcacccg gataggttgc gctgacggta atggtcggtg
     5461 gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagaccg cctgcaagca
     5521 tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga
     5581 aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt tcctggctgg
     5641 aggaaattgc tcgtgctttg atacccggac gaatgcgttg caagccggaa acgatcaccc
     5701 gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct ttgctggctt
     5761 cgatttcgcg tagctgcacg acatcgtctt tatccagaat gagtgccgtt gctttaccct
     5821 gggcgttgtg ggtgacgcct tcctgcggca ccagtaatac attctggcgg ctaccttcat
     5881 ccactaatgc cgtaacatac atgccaggca gcaagtcacc atttgggttg gggaaaatcg
     5941 cccgtaacgt cacggagccc gtggtttcat ccaccgtcgg gtcggagaat ttcagcgtgc
     6001 cggtctggcc gtaacgttta ccattttcca gattgagctg tactggcgta ctgccctgaa
     6061 cctgtttgat ttgcccactg gcgacctctt ctttcatgcg caagaaatcc tgcaccgact
     6121 gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat
     6181 tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg
     6241 aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg
     6301 tgacattggc ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa
     6361 catagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg
     6421 tagagagcgc tttcgccagc gagcctttgg cggagttcag ctctgcctgt aaaggcgcag
     6481 gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt
     6541 taatgataat tccgcccacc tggggacgta tctcggcaac ttcataagga acggttctac
     6601 cgggcaattc gctcaacaca ttgaccgaac cgggggagag tgtgacgaca ccgacctcag
     6661 gcgtcatggc ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc
     6721 agaataacaa cggtattaac agttttcttc ttctgttcat tttagtccct gaaaattctt
     6781 gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc
     6841 ggcatagtgg ggttgtcagc cagtaagatg ggatgcaagc acagtgatcg acatggtgag
     6901 gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttt ctgccagcat tcgggcaacg
     6961 aagggttcta ctggtggata cacataccag gggaataaag tcgttttcgt ctctccttca
     7021 ataatcaccg ggggtattga aatcagcatc tgcatccctc gtcatgccag ccatcaattt
     7081 cagttgctta tgtccttact aaaaataaga tgtgataccc agggtgacga tgtcgctcat
     7141 acgtcttaat ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga
     7201 aatgttgagc gttgacgtga tattgctttc atttttgttt ttcagaatca gttgcataat
     7261 tttgaactga ttatgtgaaa aacatagttg tgaattctta tggggcaagt gtttaccata
     7321 agtaatctca agaagatttt tgatactttc tttacggctg acaaaataaa taggcgcttt
     7381 agcttttagt ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt
     7441 ctccaggaat ttaatcgctt cttcatcaag gatatggttg tgataggtgt caatgatcac
     7501 cttgaagggt ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc
     7561 gtgattatct ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa
     7621 aatcataact tgctccttag ccgttatcgt ttcgaacatg tcatcctttt cattatttac
     7681 atccttgtcc gaatcgttgt tcaatatagt aaacgcctat aagaactttg cccttcgtga
     7741 actaatacgt ctattaacac taatcaatac ttcagattta tccatcacgc actccatata
     7801 ttggtcctaa gtaagattcc tggttgttat cagtttgtaa ctagtaaaca aaaggttaac
     7861 ttttgcggat tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat
     7921 attactaaag ttaaaatgta atccgattta aatatcgagt ctccttgttt cgacttaggc
     7981 tggcaattgg attgccagct ttcttttaac gcattgaaat tacctgatta aataactcat
     8041 tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca atgtaatcaa
     8101 cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc atcaacgctt
     8161 acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg accacggaaa
     8221 atatcagcca tgataatgtt tgaaatggct aatttgccat ggaacgaaaa aaatcagatt
     8281 aaattcagta ggttgaaata atcaacagca ggcaattgtt tcaatgatag tgcgtaattg
     8341 aactgaaaca ctgtatagct aagagagtat taactggatc atagtcactt aatgtgacta
     8401 tgatccaggt ggaaataggg ataattattg ctttttaacg aacaaactgg cgaagctgaa
     8461 cagactggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca gcgttaccga
     8521 caccatcggc gtagcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac
     8581 gagctgtagc caactgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac
     8641 ccctgccaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctctgg
     8701 tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa
     8761 taccggccag aaattatgac tgcggttgct gaatagcccg acaataagcg caatgcccga
     8821 gcagattaat aatgcaccca ctactgtgct taaaatgtcg ccagagacga acggaaaact
     8881 gatacacagc aacccgacga taaacagcag cacggcaata aactggattg ctctgcgatg
     8941 ttttttaagc atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga
     9001 accaccctat aaaattaaga agaaaatccc ctgctat
//
LOCUS       DAPQZD010000190         9025 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPQZD010000190 DAPQZD010000000
VERSION     DAPQZD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.192"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            170..1312
                     /locus_tag="RO454_004307"
     CDS             170..1312
                     /locus_tag="RO454_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0936046.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1319..2083)
                     /gene="glcC"
                     /locus_tag="RO454_004308"
     CDS             complement(1319..2083)
                     /gene="glcC"
                     /locus_tag="RO454_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDY0936047.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2334..3833
                     /gene="glcD"
                     /locus_tag="RO454_004309"
     CDS             2334..3833
                     /gene="glcD"
                     /locus_tag="RO454_004309"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDY0936048.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3833..4885
                     /gene="glcE"
                     /locus_tag="RO454_004310"
     CDS             3833..4885
                     /gene="glcE"
                     /locus_tag="RO454_004310"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDY0936049.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4896..6119
                     /gene="glcF"
                     /locus_tag="RO454_004311"
     CDS             4896..6119
                     /gene="glcF"
                     /locus_tag="RO454_004311"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDY0936050.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6124..6528
                     /locus_tag="RO454_004312"
     CDS             6124..6528
                     /locus_tag="RO454_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDY0936051.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6550..8721
                     /gene="glcB"
                     /locus_tag="RO454_004313"
     CDS             6550..8721
                     /gene="glcB"
                     /locus_tag="RO454_004313"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDY0936052.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHSILTKEQVQASLENMAKVVDQQNAGDPAYRPMSG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         2145 a         2266 c         2570 g         2044 t
ORIGIN      
        1 taaatcgcta aaaggcattg tggcgcttct tcgttggctg tgatcttatc gtaatcatta
       61 ataaataata tttattctta ttttgtattc atggtgttga ttattaagat gccgttcgtt
      121 gctgttgcaa cctgtttttc atcgttaatt atttgttatc cacagagata tggaatgcga
      181 tctgctgatg attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc
      241 ttttccgtca tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg
      301 caacgagcat ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt
      361 ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg
      421 cgagcgttac ggcaaagata ccggtcattt cggcatgatt gacgccattg atgatccgca
      481 ggtttttgcc gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa
      541 gatcagcggt cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt
      601 cgatacacca ccctgtgcga tgatgccgca cggtaaaccg tggtatgccg cgcatattga
      661 acaattgggt tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct
      721 cactttctct ccggcgctaa aaaaactgat ggatcaggtg cgtaagaagg tgaccattcg
      781 ctgcatcaat cgccagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaattc
      841 tggctggcag cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg
      901 cgaccaactt aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc
      961 accctgcgcg tttattgtcg gcttgccaaa catcaacgag gcgattgccg atctgaacgg
     1021 atcgctcttt cccttcggct gggcaaaatt gctgtggcgt ttaaaagtca gcggtgtgcg
     1081 aactgcgcga gtaccgctga tgggcgtgcg ggacgagtat cagttcagcc gcatcggccc
     1141 ggtgattgcg ttgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga
     1201 tgcgctggag atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg
     1261 cattggggcg gagccgtaca agcgttaccg attgtatgaa aagcagattt gatggcagtc
     1321 acccctgctt catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga
     1381 tatcgtggag attctttttc acggtccgca catgatcgcg tgctgcgcgc tgggcgacgt
     1441 gcggcagccg ctgcaacacc gcgttgtaga tccgcgcatg ctggcgatcg atctgctttt
     1501 tttgctgtgg tcgatggtag agattattta cgctggcaaa caccgaatta aacatcaggt
     1561 cggtcaatga ttgcagcgta aactccagca cctgattgtg agaagcctga caaatggcga
     1621 gatggaaagc gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac
     1681 tggcggcgag cattttttca taacagcggg ttatcacaac aaaatcagcc tgggttccca
     1741 gcgttgccgc cagccttgcc gattcgccct ccagtaatgc gcgaacgtcg agcagatcgt
     1801 acagcgttcg cggctgcgta ctgaacagat ggatcagcgg gctggtgtcc tgcacccgat
     1861 taagccgtgc gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca
     1921 gcacggtcag cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac
     1981 gttccgaggg aagcggctga ccgaccttca gtacgccgtc gataattaac cgttcgatac
     2041 tctctgcaac cacttcgcaa atagggcgac gttcatcttt cattcccgga cctcgtgcac
     2101 aggtaggacc aatttttctg ttcttaactc gcaaaacacg cacatcacgt aagtgattat
     2161 gttacatcaa tttaacatta agttaaccaa gacaaggtca cagagctgga aaaaaaatgg
     2221 tctgaccggt agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag
     2281 aagcagcgcg aaaaaatcag ctgccacaca acacaacaaa gcgaagccta ctcatgagca
     2341 tcttgtacga agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga
     2401 tggcactgcg tgagcatgtc cctggacttg agatcctgca taccgatgag gagatcattc
     2461 cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttctgccta
     2521 agcagatgga acaggtgacg gcgattctgg ctgtctgcca ccgcctgcgt gtaccggtgg
     2581 tgacccgtgg tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt
     2641 tgttggtgat ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgtgcgc
     2701 gcgtgcagcc gggcgtgcgt aacctggcga tctcccaggc cgttgcgccg cataatctct
     2761 attacgcgcc ggatccgtcc tcacaaatcg cctgttcgat tggcggcaat gtggcggaaa
     2821 atgccggtgg cgttcactgc ctgaaatatg gtctaaccgt acataacctg ctgaaaattg
     2881 aagtgcaaac gctggatggc gaggcgctga cgctgggatc ggacgcgctg gattcgcctg
     2941 gttttgacct gctggcgctg tttaccggat cggaaggtat gctcggcgtg accaccgaag
     3001 tgacgttaaa actgctgccg aagccgcccg tggcgcgggt gctgttagcc agctttgact
     3061 cggtagaaaa agccggactt gcggttggtg acatcatcgc caatggcatt atccccggcg
     3121 ggctggagat gatggataac ctgtcgatcc gcgctgcgga agattttata catgccggtt
     3181 atcccgtcga tgccgaagcg attttattgt gcgagctgga cggcgtggag tctgacgtac
     3241 aggaagactg cgagcgggtt aacgacatct tgttgaaagc gggcgcgact gacgtccgtc
     3301 tggcacagga cgaagcagag cgcgtacgtt tctgggccgg ccgcaagaac gcattcccgg
     3361 cagtggggcg catttccccg gattactact gtatggatgg caccatccca cgccgcgcgc
     3421 tgcctggcgt actggaaggc attgcccgtt tatcgcagca atatgattta cgcgttgcca
     3481 acgtctttca tgccggagac ggcaacatgc acccgttaat ccttttcgat gccaacgaac
     3541 ccggtgaatt tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag
     3601 ttggcggcag catcagtggg gaacatggca ttgggcgcga aaaaatcaat caaatgtgcg
     3661 cccagttcaa cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg
     3721 atggtttgct gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg
     3781 ccatgcatgt gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg
     3841 cgagtgtgat tacagccagg cgctgctgga gcaggtgaat caggcgatta gcgataaaac
     3901 gccgctggtg attcagggca gcaatagcaa agccttttta ggtcgtcctg tcaccgggca
     3961 aacgctggat gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat
     4021 aaccgctcgt gccggaacgc cgctggtggc gattgaagcg gcgctggaga gcgccgggca
     4081 aatgctcccc tgcgagccgc cacattatgg tgaagaagct acctggggcg ggatgatcgc
     4141 ctgcgggctg gcggggccgc gtcgcccgtg gagtggttcg gtccgcgatt ttgtcctcgg
     4201 cacgcgcatc attaccggca ctggaaaaca tctgcgtttt ggtggtgaag tgatgaaaaa
     4261 cgttgccgga tacgatctct cacggttaat ggccggaagc tacggttgtc tgggtgtgct
     4321 cactgaaatc tcgatgaaag tgttaccgcg accacgcgcc accctgagtc tgcgtcggga
     4381 aatcagcctg caagaagcca tgaatgaaat cgcccagtgg caactccagc cattacccat
     4441 tagtggctta tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc
     4501 ggtaaaagca gcgcgtgaac tgctgggggg cgaagaggtt gcgggtcagt tctggcagca
     4561 attgcgtgaa caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcctt
     4621 acccagcgat gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc
     4681 gttacgctgg ctgaaatcga cagccgatga caatcaaatc catcgcattg cccgcaacgc
     4741 tggcggtcat gcgacccgct ttagtgccgg agatggtggt tttgccccgc taccggctcc
     4801 tttattccgc tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaatcc
     4861 cggtcgcatg tacgcggagc tttaaggagc aggctatgca aacccaatta accgaagaga
     4921 tgcggcagaa cgcgcgggcg ctggaagccg acagcatcct gcgcgcctgt gttcactgcg
     4981 gattttgtac cgcaacctgc ccaacctatc agctcctggg cgatgaactg gacgggccgc
     5041 gcgggcgcat ctatctgatt aaacaggtgc tggaaggcaa cgacgtcacg cttaaaacac
     5101 aggagcatct cgatcgctgt ctcacttgcc gcaattgtga aaccacctgt ccttctggtg
     5161 tgcgctatca caatttgctg gatatcgggc gtgatattgt cgagcagaaa gtgaaacgcc
     5221 cactgccgga gcgaatgctg cgcgaaggat tgcgccaggt agtgccgcgt ccggcggtct
     5281 tccgtgcgct gacgcaggta gggctggtgc tgcgaccgtt tttaccggaa caggtcagag
     5341 caaaactgcc tgctgaaacg gtgaaagcta aaccgcgtcc accgctgcgc cataagcgtc
     5401 gggttttaat gctggaaggc tgcgcccagc ctacgctttc gcccaacacc aacgcggcaa
     5461 ctgcgcgagt gctggatcgt ctggggatca gcgtcatgcc agctaacgaa gcaggctgtt
     5521 gtggcgcggt ggactatcat cttaatgcgc aagagaaagg gttagcgcgg gcgcgcaata
     5581 atattgatgc ctggtggccc gcgattgaag caggtgccga ggctattttg cagactgcca
     5641 gcggctgcgg cgcgtttgtc aaagagtatg ggcagatgct gaaaaacgat gcgttgtatg
     5701 ccgataaagc gcgtcaggtc agtgagctgg cggtcgattt agtcgaactt ctgcgcgagg
     5761 aaccgctgga aaaactggca attcgcggcg ataaaaaact ggccttccac tgcccgtgta
     5821 ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa agtgttgctt cgtcttggat
     5881 ttaccttaac ggacgttccc gacagccatc tgtgctgcgg ttcagcggga acatatgcgt
     5941 taacgcatcc cgatctggcg cgccagctgc gggataacaa aatgaatgcg ctggaaagcg
     6001 gcaaaccgga aatgatcgtc accgccaaca ttggttgcca gacgcatctg gcgagcgccg
     6061 gtcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca agcccttgaa aaggaataac
     6121 aaaatgaaaa ctaaagtcat tcttagccag caaatggcga gtgcaattat tgccgcaggt
     6181 caggaagagg cgcagaaaaa caactggtct gtttccattg ctgttgccga tgacggcggt
     6241 catctgctgg cgttaagtcg catggacgat tgcgcgccga ttgcggctta catctcccag
     6301 gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta aggggtatga agagatggtg
     6361 aacaacggac gtaccgcgtt cgtgactgcg ccgttattaa cgtcgctaga aggtggcgta
     6421 ccggttgttg tggatgggca aattattggt gccgtgggcg tttctggttt aaccggagca
     6481 caggatgcgc aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc gaaaacgagg
     6541 agagaaacaa tgagtcaaac cataacccag ggccgtttac gcattaacgc caattttaaa
     6601 cgttttgtgg atgaagaagt tttaccggga acagggttgg acgctgcggc gttctggcgc
     6661 aattttgatg agatcgttca cgatctggca ccagaaaatc gtcagttgct ggcagaacgc
     6721 gatcgcattc aggcggcgct tgatgagtgg catcgcagca atccggggcc ggtaaaagat
     6781 aaagcggcct ataaatcttt cctgcgtgaa ctgggctacc tggtgccgca accggagcgc
     6841 gtaacggtgg aaaccacggg cattgacagc gaaatcacca gtcaggcggg gccacagctg
     6901 gtggttccgg caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg ctggggctca
     6961 ctgtacgatg cgttatacgg cagcgacatc atcccgcagg aaggggcgat ggtcagcggc
     7021 tacgatccgc agcgtggtga gcaggttatc gcctgggttc ggcgtttcct cgatgaatca
     7081 ctaccgctgg aaaacggcag ctatcaggat gtggtggcgt ttaaggtggt cgataaacaa
     7141 ttacgcatcc agttgaaaaa tggtaaagaa accacgttac gtaccccggc gcagtttgtc
     7201 ggttaccgtg gcgatgccgc tgcgccgacc tgcattttgc tgaaaaataa cggtctgcat
     7261 attgaactgc aaattgatgc caacgggcgg attggcaaag acgatccggc gcatatcaac
     7321 gatgttatcg tcgaagcggc catcagtacc attctcgact gcgaagattc ggtcgcggcg
     7381 gttgatgcgg aagataaaat cctgctgtac cgcaacctgc tgggcctgat gcaggggact
     7441 ctgcaagaga aaatggaaaa aaacggtcgg caaatcgtgc gtaaactgaa tgacgatcgt
     7501 cattacaccg ccgccgatgg ctctgaaatt tctctgcacg gacgctcgct gctgtttatc
     7561 cgcaacgtgg gtcatttgat gaccattcct gtgatttggg acagcgaagg caatgaaatc
     7621 ccggaaggca ttcttgatgg cgtcatgact ggcgcgattg ccctctatga tttaaaagtg
     7681 cagaaaaact cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca cggtccgcag
     7741 gaagtggcgt tcgccaacaa actgtttacc cgcattgaga caatgctcgg tatggcaccg
     7801 aataccctga aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa cttgcgtagc
     7861 tgtatcgctc aggcgcgtaa ccgcgtggcg ttcatcaata ccggtttcct cgaccgtacc
     7921 ggcgatgaaa tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa ccagatgaaa
     7981 tcgacgccgt ggatcaaagc ctacgaacgt aataacgtgc tttccggtct gttctgtggg
     8041 ctgcgcggta aagcgcaaat cggtaaaggg atgtgggcaa tgccggacct gatggcagac
     8101 atgtacagcc agaagggcga ccaactgcgt gccggggcaa acacagcctg ggttccgtca
     8161 ccaaccgctg ctacgctcca tgcgctgcac taccaccaaa ccaacgtaca gagcgtgcag
     8221 gccaacattg cccagagcga gttcaatgct gaatttgaac cgctgctgga cgatctgctg
     8281 actattccgg tggctgaaaa cgctaactgg tcggttgaag agatccaaca agagctggat
     8341 aacaacgtgc aggggattct ggggtacgtg gtgcgttggg tggagcaggg gattggttgt
     8401 tcaaaagtgc cggatattca caacgtggcg ctgatggaag accgcgcaac gctgcgtatc
     8461 tccagccagc atatcgccaa ctggttacgt cacagtattc tgaccaaaga acaggtgcag
     8521 gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa acgctggcga tccggcttat
     8581 cgtccgatgt cggggaattt cgctaactcg tgtgctttta aagctgccag cgatttaatc
     8641 ttcctcggcg tgaaacagcc aaacggctat accgaaccgt tattacacgc ctggcgttta
     8701 cgcgaaaaag aaagtcatta atagcgtcaa aaaagccccg gcaagtttta ttgtcggggc
     8761 tatgatagta actgcggtag tacatttatc ccggaaataa ccggagcgat tatcagggtt
     8821 taacaatatt aaatacccgt agcgcattac tagcgggcct aaaacaacaa tatggacatc
     8881 ttaacgatat tctgcgcctg cgtgtgcgga acagaaaccc ttacccgata tacttgttat
     8941 gccagaggta gcaattgcgt tagcagcgta ctgttgctgt aaagtataac gcctatattt
     9001 tacagttcag tcctcttttg cttat
//
LOCUS       DAPQZD010000191         8916 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPQZD010000191 DAPQZD010000000
VERSION     DAPQZD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.193"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            429..626
                     /locus_tag="RO454_004314"
     CDS             429..626
                     /locus_tag="RO454_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936053.1"
                     /translation="MTVFLMAVFYGLLKTGSLIALLVDAYKGWTLFWKNYDRLPHIYR
                     SQWRYQRRFILKRIWKRIRTG"
     gene            complement(623..907)
                     /locus_tag="RO454_004315"
     CDS             complement(623..907)
                     /locus_tag="RO454_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="korC"
                     /protein_id="HDY0936054.1"
                     /translation="MKAENARQVQGLIELEKFNPETLCSGESWMAPSASEVSVVRALI
                     PLTDIQLANRLDVDERTIRKWKSGETRMVFTTWCCLCWLAGLGMLLEEPA"
     gene            complement(927..2060)
                     /locus_tag="RO454_004316"
     CDS             complement(927..2060)
                     /locus_tag="RO454_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDY0936055.1"
                     /translation="MHFYVDETGQTGRNLFDKTQPILSYGVLSSEADLDKVAEADLAA
                     LRKTLGVERLHAAELGVHRLSEVVDTLLVLQKNHRIRFDIWQVVKRDHAIISFFDQVF
                     DQGLNPVVPWSAYWTPLRYPLLLNLSNLFDDELAEKAWRARLEAHDERSCSLFSEVCG
                     VLLQRVHSLGDARSVELITDALSWAMVNFDELGYNCKTNKEKLQIMPNMIGFQSVLHG
                     ICSRLGAPNRKADIIVDQQSQFNTTQRELNEFYYQIREQPWALGPGLPVMDMKNMPAK
                     PLVFQSGTMSAGLELVDIYLWIFKRYMERKELTKPLSRLVYTNLKTARTDSVSLQSVA
                     KRFKEFFEKLSEPTAEMIEKANELRAVEETRRLAHRVQSVSQS"
     gene            complement(2323..5442)
                     /locus_tag="RO454_004317"
     CDS             complement(2323..5442)
                     /locus_tag="RO454_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936056.1"
                     /translation="MFGKLLKSVSWQVRAELRRSLKSNRDYKKLRWNPVERILVSCST
                     HYVRAMLVLWSAAFGAVGVVEYFRPVLLPFAVQHFKGITKLSDWMSNLLGSQLTIIGI
                     VFPLVVGLISVLFQKKSARIHIQSAYQLHSGYMFAGLSGLSLAAFVVLGGMTLSIGDG
                     YLNTSFAVTAFVWMLFNIILSIWFFVSSLNVLDESKRDRLMNKFFLSQIVDDYIQKAY
                     IQAWLRYPGGHVGQNYLGNIKILPYSISEKDDMLHVKSNISKGDVVTDIYIRPFLFLL
                     RRLEAVDGQDAEIIILPSFGVRSGELTLLSSRNVKPVSGLWRWLLRRCIVTGRPENKR
                     DLDDITFDFFGEAYDALNDKNISVFRTGIERLTDTYTSIKRSYNYEVDKNYLDEVKES
                     GFSHTFSDSFHYELRKFFRESVKSTEYSGEYFRESMLIPLRVYRKTQSTCFTDFRQFL
                     LSLFRVWHVLNEWKAGLGGPLSASQELTHQALIRGYIGLWEGWSMTTITGKPGSEDST
                     GRLMYHLHNTARLLIPSVVADNASSVRYAHDVLCLWFNQSRFTRYWEEEYRWHSFFLT
                     PDYLSLKETEPQWNMLLRGSKYKKDAALSIMFANALSDLRLLMAGYLIAHFESQKNID
                     LADLVNHLIMSELYEDRDTHDTLTPAFRRSVDIIDMILRIEHCNLHTNTSWYSGLSET
                     IEVMNSYNERPYIPGRMYTGEYEDLGSLYGSFALLAIKLARPAEQVTQRVNEALAGGV
                     FSYSSKDRIISILKRLKRDPSVPYEGYIISEADYATNVVFFNDVLDKYIDVFSRSKTA
                     DIVAAEVDQARLRNTDARLTNELPGALSEDVLLKYFTFTQNSECDRNWLAIYIPVGVS
                     KEYVARELNQTDYGDFPSVSEVNRNILRRLHYVLWQSQAKLTIEVNNLETLLMEVAQR
                     SADQNNYILVIYGSRFSEELRELVYQPERHDAFSIHVDVSARGSRSLPFRINNCLIYL
                     VLNSEQEFSLMVSAESFGELRLFRYPDGTLFNTFYRSSDDPLEGVMKTLWEIEMEITD
                     TPVARFEHR"
     gene            5749..5979
                     /locus_tag="RO454_004318"
     CDS             5749..5979
                     /locus_tag="RO454_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDY0936057.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            5976..6392
                     /locus_tag="RO454_004319"
     CDS             5976..6392
                     /locus_tag="RO454_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDY0936058.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDECCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(6554..8692)
                     /locus_tag="RO454_004320"
     CDS             complement(6554..8692)
                     /locus_tag="RO454_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0936059.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTFQPDELISD
                     SFNILIRNQAHAEREREIEEMTQKIRAVFTDHTELNSLIDHLQELSNAFKSTSSGISR
                     SSTGMRGLSGGNKIHHIPAGLENYQPYIRSERRVEWIDWQTKGLEFSPLSDGCCPFCT
                     GDITGKEAQIRQVREEYDKSTIKNLTAIIRLVENLGNYLTESARERLLAITMLQNGPE
                     AEHIEYLVALKRQTDTLTEKLTALRGLNVFSLQEQQNVREVLTARLIDLQFFPDLQSE
                     LMQGITDRLNAALMDLINLAGPLQGKINRHRDSMIRLIAQHKTNINNFLTYAGYKYRV
                     DIAGEGEQRKLRLRHIDFDGYVSGGSQHLSYGERNAFAIVLFMYECLSKNPGLIILDD
                     PISSFDKNKKFAILEMLFRRASGECLKNRTVLMLTHDVEPVIDTLKSVRRLFSNQVTA
                     SCLRLSAGVIEELPVNDGDIMTFMQICKSITASADCEEIIKLIYLRRYFEIVDERGDA
                     YQLLSNLFHRRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALV
                     SSPDEIKNLYRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRF
                     DLIPEYVIMECDKLIALPPAANQSSVARIA"
BASE COUNT         2390 a         2180 c         2046 g         2300 t
ORIGIN      
        1 actttatttg aataaacgac tttccggaag tgtacaacgc tgagaaaatc atcatatcgc
       61 tgttttccgc ccctgcacgg cgcatcacag gtcagggaaa taacacaaca tatagtgtca
      121 tcagcagctg aatccggcac cacatgttgt gcttacagga agtggcgttg tgtggggggg
      181 agaggaagat ccgtaactgc cggaaaccgg atctcagaag agaaaaagaa gagttgttgc
      241 aagataagca caaaaaatgg tggtttgttg agatatggta attattgctc agtgggcaaa
      301 attcagaaca gagttagcct gaaattgcaa aaaacaggcc agaaaaactt ttttcagctc
      361 attttcatca accggcctta aaggctgctt cggatttcct aaatacagtt ttacataagg
      421 aacatcgtat gactgtattt ctcatggccg ttttctatgg cctcttaaaa actggctctc
      481 tcattgccct acttgttgat gcctataaag gctggacctt gttctggaag aactatgatc
      541 gactccccca tatctaccgt tcgcaatggc gatatcagcg acgcttcatt ctcaaaagaa
      601 tctggaaacg catcagaacc ggctaagccg gttcttccag cagcatcccc aacccggcca
      661 gccagcacag acagcaccag gtggtgaaca ccattctggt ctcgccggat ttccacttac
      721 ggatggtgcg ctcgtccaca tctagccggt tggccagctg gatatctgtg agcgggatca
      781 gcgcccggac cacgctgact tcactggctg aaggtgccat ccagctctcg cccgagcaca
      841 gtgtttcagg gttaaatttc tctagttcta tcagcccctg aacctgacgg gcattttctg
      901 ccttcatatg cggttaacct ttctgatcag gattgcgaca cactttgtac acgatgggca
      961 agacggcgcg tttcttccac ggccctgagt tcatttgctt tttctatcat ttctgcggtt
     1021 ggttcagaaa gtttttcaaa aaactcctta aacctctttg caactgactg cagagaaaca
     1081 ctgtcagttc tcgctgtttt aagattggtg tagacgaggc gggaaagggg tttggtcagt
     1141 tccttccgtt ccatgtatcg tttgaagatc cagagataaa tatcaaccag ttccagcccg
     1201 gcactcatcg ttcccgactg gaagacaagc ggttttgctg gcatgttctt catatccatg
     1261 acaggcaaac ccggccccag tgcccacggc tgttcgcgga tctggtagta aaactcgttc
     1321 aactcccgct gggtggtgtt gaactgtgac tgctggtcaa caatgatatc tgctttccgg
     1381 ttcggtgccc ccagacgaga gcagatcccg tgcaaaacgg actgaaaacc aatcatattg
     1441 ggcatgatct gcaacttttc cttattggtt ttgcagttgt accccagctc atcaaagttg
     1501 accatcgccc aagatagggc atcggtaatg agttcaactg aacgggcatc acccagagag
     1561 tgaacgcgct gaagcagcac tccacaaacc tcactgaata atgagcaaga acgttcatca
     1621 tgagcctcaa gacgggcacg ccacgctttt tcagccagct catcatcaaa cagattagac
     1681 agattcagca gcaggggata gcgcagaggg gtccagtagg cactccaggg cacaaccgga
     1741 ttcagtccct gatcaaacac ctggtcgaag aacgaaataa tggcgtgatc gcgcttaacc
     1801 acctgccaga tatcaaaccg tatccggtga tttttctgca ggaccagcag cgtatccaca
     1861 acttctgaaa ggcggtgcac gcccagttcc gcagcgtgca ggcgctccac gcccagagtt
     1921 ttgcgtaatg cggcaagatc cgcttcagca accttatcta ggtcagcctc tgaggacagt
     1981 actccgtagg agaggattgg ctgggttttg tcaaaaaggt ttcggccagt ctggcctgtt
     2041 tcatccacat aaaagtgcat tcagatatcc ttttcaaggc aggaggaaaa gaaacggcat
     2101 aattcaaatc aatgtaaaac cttcctgttg ccggctgcaa gtgatcctct taaatgagtt
     2161 ttcggatcta aggaagagag aagagggatt attgtatgaa tggcacaaaa agcgacggat
     2221 tgttgcggtg aatagattat tgcttaggaa gcaaaattta tagtaattga taactctaat
     2281 gcaattaaac agcgacccca tcaggggtca ccgtaatttt gatcagcgat gttcaaaccg
     2341 cgccactgga gtatcagtga tttccatctc tatttcccag agcgttttca tcacaccttc
     2401 aagtggatcg tcgctgctgc ggtagaaggt attgaaaagg gttccatccg gatacctgaa
     2461 cagcctgagt tcaccaaagg attcagcact gaccataagc gaaaactcct gttcggaatt
     2521 gaggacaagg taaataagac agttattgat cctgaagggc agagagcggc tacccctggc
     2581 cgatacatca acatgtatgc taaacgcgtc atgcctttcc ggctgataga ccagttccct
     2641 cagttcctcg ctgaagcggg aaccatagat caccagaatg tagttattct gatcagcgga
     2701 ccgttgcgcg acttccatta acagcgtttc gaggttattg acctcaatcg ttaatttagc
     2761 ctgtgactgc cacagtacat aatgcagtct gcggagaata tttctgttca cttctgatac
     2821 cgaagggaaa tctccgtagt cagtctgatt aagctctctg gcaacatact ctttggaaac
     2881 accaacagga atatatatcg ccagccagtt gcgatcacat tctgaatttt gagtaaacgt
     2941 gaagtattta agcagaacat cttcagacaa tgccccagga agttcatttg taagacgagc
     3001 atcagtgttt ctcagtcgag cctggtctac ttcagcagca acgatatctg ccgttttact
     3061 acgactgaat acatctatgt atttatccag aacatcatta aagaacacca cattcgttgc
     3121 ataatccgct tctgaaatga tataaccttc atagggaact gaagggtctc gtttcagtcg
     3181 tttaagaata gaaattattc tgtctttact ggaataggaa aacacccctc cagccagggc
     3241 ttcattaaca cgctgtgtaa cctgctccgc aggccttgcg agctttatag ccagcaaggc
     3301 aaacgacccg tagagactcc ctaaatcttc atattcccct gtatacatac gcccgggaat
     3361 gtaaggcctt tcattatatg agttcataac ctcaatggtt tctgataacc cgctgtacca
     3421 gcttgtattc gtatgcagat tgcagtgctc aattctcagt atcatgtcaa tgatatcgac
     3481 agaacgtctg aatgcaggcg tcagcgtgtc atgtgtatct ctatcttcat ataattcaga
     3541 cataatgaga tgattcacaa gatcggctaa gtctatattt ttttgcgatt caaaatgagc
     3601 aataagatac cctgccatta acaaccgcaa atcggataag gcatttgcaa acataatgga
     3661 caaagcggca tcttttttat acttgctgcc cctgagtaac atattccact gcggttctgt
     3721 ctccttcagg gacagataat ccggagtcag gaagaaactg tgccagcggt actcttcctc
     3781 ccagtaacgt gtaaaccggc tctgattaaa ccagaggcag agaacgtcgt gagcatatcg
     3841 cacggaagag gcattatcgg caaccactga cggaataaga agcctggcgg tattatgcag
     3901 atgatacatc aggcggccag tagaatcttc actcccgggt ttgccagtga tcgtggtcat
     3961 actccagcct tcccacagtc cgatataccc gcggataagg gcctggtgag tcagctcctg
     4021 gctggccgag agggggcccc ccagtcccgc tttccactcg tttaacacat gccacaccct
     4081 gaaaagcgag agtaaaaact gtctgaaatc ggtaaaacac gtgctctgtg tttttctgta
     4141 cacacgcaat ggaatgagca tcgattcacg gaaatattcg cctgaatatt ccgttgattt
     4201 tacggattca cgaaaaaact ttctcaattc ataatggaat gaatcgctga aagtatggct
     4261 aaaacctgat tcttttactt catccagata atttttatcg acctcgtaat tgtaacttct
     4321 tttgatagag gtataggtat ctgttaatcg ttcaatccct gttctgaaga cgctaatatt
     4381 tttatcgtta agcgcatcgt aagcctcacc aaagaaatca aaagtaatat catccagatc
     4441 ccttttgttc tcagggcgcc ctgttacaat acaacgacgc aataaccacc gccacaatcc
     4501 tgaaacaggc ttgacatttc tcgatgacaa taaagtcagt tcaccagaac gaacaccaaa
     4561 ggagggcaga atgataattt ccgcatcctg cccatctacc gcctctagcc ttctcagtag
     4621 gaataaaaaa ggccttatat aaatatcagt aacaacatca cctttactga tattagattt
     4681 aacatgaagc atatcgtctt tctcagaaat tgagtaaggc agtattttaa tattaccgag
     4741 gtaattttga cctacatgac cccccgggta acgcagccac gcctgaatat atgctttttg
     4801 aatgtagtca tccactatct gagataaaaa aaacttattc attagtcgat cgcgtttgct
     4861 ctcatccagg acattcagac tggagacgaa gaaccagata gacagaataa tattgaaaag
     4921 catccacaca aaagctgtta cggcaaaaga agtattaaga tatccgtcac ctatagataa
     4981 cgtcataccg cccagcacga caaatgcagc cagtgaaaga ccactcaggc ccgcaaacat
     5041 atatccggaa tgaagttgat acgcggactg aatatggatc cttgctgatt tcttttgaaa
     5101 taaaacactt ataagcccca caaccagcgg aaatacaata ccgattatag tcaactggct
     5161 acccagtaaa ttagacatcc aatctgaaag cttggtaatg cccttaaaat gctgcacagc
     5221 gaaaggtagc aatacagggc gaaaatattc gaccacgcca acagccccga aggctgcgga
     5281 ccatagaacc agcatagccc ggacgtaatg ggtggagcag gaaacaagta ttctctccac
     5341 cgggttccag cgaagttttt tgtaatcccg gttcgacttc aggctccgtc ggagttcagc
     5401 tctcacctgc catgagacac ttttcagaag ttttccaaac atagatatcc tgcgtattac
     5461 ataagaaatt ttatgaataa taagcggtag gaaccttttt ttctctggaa aatgtcaccc
     5521 gatacccttc atacaggctt tgtcatccta aaatccggtt taaccttttt tcggatctcc
     5581 gataaaaaag aaagaaatag agatatgcat aaacatatgc ctggcatcta catatgctac
     5641 cgctatacat atgccacaca tctgcctata ctaaacatat gcacacaccc ggtgctttga
     5701 atattccgga tatgtttcac atatccatat gctcgggaga gaaagaacat gagaaccgta
     5761 tcgatattta aaaatggcaa caaccgcgcc atccgtctgc ctcgtgatct ggattttgag
     5821 ggggtgagcg agctcgagat cgtccgggaa ggggacagca tcatcctgcg ccccgtccgg
     5881 ccaacctggg gatcattcct agagtacgaa aaagccgatc cagattttat ggcggagcgc
     5941 gaggatgttg tcagcgacga agggcgattt aacctgtgaa caagacttat atgcttgata
     6001 cctgtatctg ctcgttcatc atgcgtgaac agccagaagc cgtcctgaag cgtctggagc
     6061 aggcggtgct acgcggccac cgtatagtgg tctcggccat cacctactct gaaatgcgct
     6121 tcggggctac cggcccaaag gcctcaccac gtcacgtcca actggtcgat gaatgctgcg
     6181 cccgcctcga tgccatcctg ccctgggatc gcgccgcagt agacgccacc acgaagatta
     6241 aagtggcact gcggctggcc gggacgccga tcggcccgaa cgacacggcg attgccgggc
     6301 acgccatcgc cgccggggcg atactggtaa cgaataatac aagagaattt gagcgagtgc
     6361 ctgacctggt gctggaagac tgggtgaagt aagacactct tttcaaagtc aggagataag
     6421 ggcaccaaga aacagcccgt atacgggctg ttttaacaga tatctacagt aagcggtcct
     6481 aaactgtagg gtatcttcga agatcagact atcagggagc taaagcctat cttccggcgg
     6541 caaaaacagt ggctcaagcg atacgggcaa ccgagctctg gtttgctgcc ggtggcaggg
     6601 cgatgagctt gtcacattcc ataatcacgt actccgggat gagatcgaat ctggacggat
     6661 ctagttgaca gatgaactca ttttcaatgt ggtacgtttc gttaacgaat tttcgtatta
     6721 ccggatggtc ctgatccagt tccagtaaac ggaatacctg cagtttttca tagccgttac
     6781 gacagcgacg gtacaagttt tttatttcat ctgggctgct gacgagagcc tgaagtcgcg
     6841 ggtaatcaaa actgtccaca tactcccgga tatcccgcaa ggcctgctga attttctcag
     6901 gagccatttt gggatagccc gagcccgcag cggcaggctc gcgataatcc agcggggcga
     6961 cgcgtcgatg gaatagattg gatagaagct gataagcatc accacgttcg tcaacgattt
     7021 caaagtagcg acgaagatag atgagcttaa tgatctcctc acagtctgct gacgcggtga
     7081 tggatttgca gatctgcatg aatgtcatga tatcgccgtc gttaacaggt aattcttcta
     7141 tgactccagc agacaggcgc aggcaggagg cggtcacctg attactgaac aaccttctga
     7201 ctgacttcag cgtgtcaatc acaggctcca cgtcatgcgt cagcataagt acagtccggt
     7261 tcttcaggca ttcaccactt gcacgtctga acagcatttc aagaatggca aacttcttgt
     7321 ttttatcaaa agaagatatg ggatcatcaa ggataatcag tcccgggttt tttgacaggc
     7381 attcatacat gaataacaca atcgcaaagg cgttccgttc tccatagctg agatgctggc
     7441 taccgccgct gacgtacccg tcaaaatcta tgtgtcgtag cctgagtttt ctctgctctc
     7501 cctcgccggc tatatccacc ctgtatttat agcctgcata agtgaggaaa ttattgatgt
     7561 ttgttttgtg ctgtgcgatc agccggatca tgctgtcccg gtgccggtta atttttccct
     7621 gcagcggtcc ggcaaggttt atcaggtcca taagggccgc gttcagtctg tcggtgatcc
     7681 cctgcatgag ttcggattgc agatcaggga agaactgcag gtcaatcaac ctggcagtga
     7741 gcacctcccg aacgttctgc tgttcctgca ggctgaaaac attcaggcct ctcagcgcag
     7801 tgagtttctc tgtcagcgta tcggtctggc gtttcaacgc cactaggtac tcgatatgtt
     7861 cggcttccgg accgttctga agcatagtaa tggccagaag gcgttctctg gcgctctccg
     7921 tcaggtaatt accgaggttt tccaccagtc tgatgatagc ggtcaggttt ttaatggtgg
     7981 atttatcgta ttcctctctg acttggcgaa tttgtgcttc ctttcccgtg atatcaccgg
     8041 tacagaacgg acagcagcca tccgaaaggg gcgaaaactc gagtcccttt gtctgccagt
     8101 caatccattc cacgcggcgt tcactgcgga tgtagggttg atagttctca agaccagccg
     8161 gaatgtggtg tattttattt ccgcctgaca gacctctcat gccggttgaa gaacgtgaaa
     8221 tcccggaact ggttgatttg aaagcattac tgagttcctg tagatggtct atcagagagt
     8281 tcagctcagt gtgatccgtg aaaacagccc ggatcttttg cgtcatttct tctatttcac
     8341 gctctctttc cgcatgggct tgattgcgga taagaatatt aaagctgtca ctgatgagct
     8401 cgtccggttg gaacgtaaac tgactgacgt actcctcgtt gaagcacatc acgtccccaa
     8461 tcccgtcagc accgatgaca atgggcccgg tgctatcggg gttttctccg cgcagccgga
     8521 agggcatcag tgcctgtagt ccctgaatgt cgtcccgcgc cgcacagctt atggcccgtg
     8581 acagcgtact ttttccagta ccatttggcg caaacttaat gttcagttta tcggcagtga
     8641 gggtgatatg tgctcgtacg atgttattgc agtggcgtat ttcaatgtcc atttatctgt
     8701 cctttgccag ttattccggg gggattcata tcctgccgca aaatgtgccc gcatgctgtg
     8761 atgcagatac ataatacatt tcgtttatac cattgcttac acctctccga gatgttcata
     8821 tcagaaacaa agattaggtc gatccgactt ttactttttt cggatctctg gagaggggaa
     8881 gaaggatttg tgctgatgat gcaatactaa tgatat
//
LOCUS       DAPQZD010000192         8748 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPQZD010000192 DAPQZD010000000
VERSION     DAPQZD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.194"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1158..1334
                     /locus_tag="RO454_004321"
     CDS             1158..1334
                     /locus_tag="RO454_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936060.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(1371..3290)
                     /gene="dhaR"
                     /locus_tag="RO454_004322"
     CDS             complement(1371..3290)
                     /gene="dhaR"
                     /locus_tag="RO454_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDY0936061.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            3518..4588
                     /gene="dhaK"
                     /locus_tag="RO454_004323"
     CDS             3518..4588
                     /gene="dhaK"
                     /locus_tag="RO454_004323"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDY0936062.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4599..5231
                     /gene="dhaL"
                     /locus_tag="RO454_004324"
     CDS             4599..5231
                     /gene="dhaL"
                     /locus_tag="RO454_004324"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDY0936063.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            5242..6660
                     /gene="dhaM"
                     /locus_tag="RO454_004325"
     CDS             5242..6660
                     /gene="dhaM"
                     /locus_tag="RO454_004325"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDY0936064.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6980..8677
                     /gene="treA"
                     /locus_tag="RO454_004326"
     CDS             6980..8677
                     /gene="treA"
                     /locus_tag="RO454_004326"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDY0936065.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         2304 a         2210 c         2080 g         2154 t
ORIGIN      
        1 ctttaatctg aagcaaatca gccagttcaa atttgttaca acaagcctgc agccttgcca
       61 gataggtagg tttaaacata acagtaaatt ccattattga ttatgttaat gcacatgcag
      121 gtactcttac gcatttaacg gtgacgccaa gatgacttga cggcagtcag agggcgaata
      181 ctcccgacct tgttaacact gattgcgata ccacaaccaa caaatctacc tgcatgtgcc
      241 gtattaaatt agcatagtca tcatataact caagccccca atcaattttt ttcaaaatcc
      301 tcaataagtt atagatggtt tgcgttctgt tcatttctgg catcagagtg atgattaggc
      361 aagttagctg ggaaaattta tccggagtat atacaaagaa agtagtcagc ccagttgaat
      421 catctaaaca agaccaatta actaatttat ttaaatgaaa aataatgctt ttgagctaac
      481 caattatgca cttaagctaa atttaccgtt tttggggcta gcccaacatc gagggcgcat
      541 aagctttagc ccacttttac tttcctgttt tctttagtaa cagaaggtta actatcaaat
      601 atttttgttg ggctatgtgg gctaacagtt ttcatattta cgtgtttttt tattcttctc
      661 cggtttccgt ttcagatacc ggagtaaaca acagcgtata agcccaggga cgtgcatgct
      721 tgaggtgtct cagccgtggc atacgtatca gccatccctc cacgctggtg gactttttga
      781 gcattccagc ctcatgaagg attctgccgg cagtatcctt gctgagagga gtagaaccta
      841 cgcccttcgc attacgaatg cgagctaact ccaaaatgag tacgaacatg agtacaaaaa
      901 aaatatatca ttaaatgtag tataaatttc aatcagatac agcaaaaatt acaattcaac
      961 gtctaattcc gctaatacta ctctggcggt taataacgag ccattatgtg agttgcacgt
     1021 aatggctttt ttacgcctgc tacgcgtctt ctttcaattc tgagttacca aaactcataa
     1081 tcagactcct gacaatatta acaatgcagc ttttatgtga taacaatcac agtgcattaa
     1141 ccgttcaaga gtttgtcatg aaaaaaacat ttaccttcag cctgttagca attgtctttg
     1201 caagcctgtt gagtgcttgt gttccccacc atcatcaccg ccacagcgat ggccctcgtg
     1261 gtcctgcgcc ctccggtaag ccaatgccac cttcaaatgg tggaccaaat cacggcagtc
     1321 atggtcgcta ttaattagta attcatagcg ggaaagtaca tactctcccg ctatccacgg
     1381 cgcttaaact gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg
     1441 ccgattccaa gtaaagccga catttcctga atgcgaccgc ctgtgacttg ggctgcgtta
     1501 ataattgcct ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg
     1561 acatcatctg tcgcctgctc ggtaaacaga tgttccggca aatcactgac gcgaatgcgc
     1621 ccattatcac tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcca
     1681 ggccatgcac aggaaaccag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag
     1741 aagcgttttt caaggctgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc
     1801 atacgcagtg gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta
     1861 aaacgatttt gttccaccag cattgcgagg tccgcggtcg ttgtggcaat cactttgaca
     1921 tcaatgggta ttaaacgccg cgcatccagt cgcgtgataa caccctgctt gataacctga
     1981 agcaaagcag actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg
     2041 ccgtgcgcca gttccagccg actcagacgg ccattttcat tatccgtgcg atcgccacca
     2101 ataaattctt ccgccagcgc cgcatcacca tataactcac aattgacggc gatataaggc
     2161 cccgctgcac gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc
     2221 tcttctccac aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc
     2281 aagcggcggg tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct
     2341 aattgactgg tcattaactg ccgcatctgt tccacaggat ggagcaataa aataaagctg
     2401 gttccctgcg tttctattat cggtttaagg gttatcaccg catcaataaa ctgatgctga
     2461 ctttcaaagg ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat
     2521 acggcgggta acgttaagag ttcagtgatt gcccttccct gacttgccgt cgcgtcaagg
     2581 cgcaagactc gcgccgcctg ggcgttgata aattgcaaat taccctgctc gtcccagcta
     2641 atcacgccat catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt
     2701 tcagcgagca aactgtccgt cagcagtaaa tttccgacct cgcgtgcgat tgccaacgtc
     2761 aacggcaaat cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc
     2821 aatcggccct tgctgtcaaa caacggcgtt gcgcaaaagg cccagttcca gagtgcctgt
     2881 ttgaaatgtt gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc
     2941 gcacaagtac caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtacactt
     3001 agctgctgca aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg
     3061 ataagcaacg cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc
     3121 ccgagcgtca gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc
     3181 tggtgtggta cgttccatgt ctcccgtttc atcagcttat tgcatctctc ccaggaggtt
     3241 gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc
     3301 ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca
     3361 atatggaaca ctcattaaag catatgtttc actatgaaac ataatttggg ctaattttct
     3421 tttcccttgc ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac
     3481 atcatccatg ccctaccgta attgctggag caaaataatg aaaaaattga tcaatgatgt
     3541 gcaagacgta ctggacgaac aactggcagg actagcgaaa gcgcatccat cgctgacact
     3601 gcatcaggat ccggtgtatg tcacccgagc tgatgccccc gttgcaggaa aagtcgccct
     3661 gctgtcgggt ggcggcagcg gacacgagcc gatgcattgt ggttatatcg gtcaggggat
     3721 gctttcgggg gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga
     3781 atgcgccatg caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg
     3841 cgatattctt aacttcgaaa cagcaaccga gttactgcac gatagcggcg taaaagtgac
     3901 cactgtggtc attgatgacg acgttgcagt aaaagacagt ctttataccg ccgggcgacg
     3961 cggcgttgcc aacaccgtat taattgaaaa actcgtaggt gcagcggcgg agcgtggcga
     4021 ctcactggac gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg
     4081 tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta ccctggcgga
     4141 taatgagatg gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccctt
     4201 ctcttccctt gatcaaaccg tcgatgaaat gttcgacacc ctgctggaaa atggctcata
     4261 ccatcgcact ttgcgtttat gggattatca acaaggcagc tggcaggaag aaccacaaac
     4321 caaacaaccg ctccagtctg gcgatcgggt gattgcgctg gttaacaatc ttggcgcaac
     4381 tccgctttct gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg
     4441 attgactatc gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt
     4501 ctcaatcacc ttactgaaag ttgatgacga aacgctggca ctctgggacg ccccggtcca
     4561 caccccggcc cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa
     4621 ttgttaactg gctcactcgt tgtggcgata ttttcagcac cgagagcgag tatcttactg
     4681 gactggatcg cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttggca
     4741 aagtggtgga aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaagaata
     4801 ccggcatgac gctactttcc agcgtcggag gtgccagtgg tccgctgttc ggtaccttct
     4861 ttatccgcgc cgcacagaca acccaggcac ggcaaagcct gacgctggaa gagctttatc
     4921 agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata
     4981 aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc tgcgagcaaa
     5041 atctctctgt tccggcggcg ctcgacgctg ccagtagcat cgccgaatcc gctgcacaaa
     5101 gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg
     5161 gtcaccagga tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg
     5221 caaaagagta aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg
     5281 ggagaaggtg tcggtgaatt agcccgtcag atgttaatga gtgataattg taaaatcgcc
     5341 attgccgcgg gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg
     5401 gaggccatcg aatctgttgc cgatgccgac catgtgctgg tcatgatgga tatgggtagc
     5461 gcattattga gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta
     5521 cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg
     5581 ggggcggata tcgacaaagt tatctttgac gccatgcatg cgctggaagc caaacgtgaa
     5641 caactgggtt taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa
     5701 gaagcccgtt ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc
     5761 tcccggctgg tttatacctt atcgacattt aatgccgata tgttgctgga gaaaaacggc
     5821 aaatgcgtta caccggagag tattaaccag attgcgttac tacaagttcg ctataacgat
     5881 acgctgcgcc tgattgcgaa aggaccagaa gctgaagagg cactgatcgc tttccgtcag
     5941 ttggctgaag ataactttgg tgaaacggag gaagttgctc cacctactct gcgtcccgtt
     6001 ccgcctgttt cgggtaaagc cttttattat caacctgttt tatgtacggt acaggcaaaa
     6061 tcaaccctga ccgtggaaga agaacaagaa cgattacgcc aggccattga cttcacgtta
     6121 ttagatctga tgacgttaac agcgaaagca gaaaccagcg ggcttgacga tattgccgca
     6181 atcttttctg gtcaccatac actgttagat gatccggaac tgcaggcggc ggcaagcgaa
     6241 ctccttcagc atgaacattg cacggcggaa tatgcctggc agcaagttct taaagaactt
     6301 agccagcaat accaacagct ggatgatgaa tatctacagg ctcgctatat cgatgtggac
     6361 gatcttctgc atcgcaccct ggtccacctg acccaaacga aagaagaact cccgcagttt
     6421 aactcgccaa ctattctact ggcggagaac atttatccct ccacagtact gcaactggat
     6481 ccagcggttg taaaaggtat ctgccttagc gccggaactc cgctatccca cagtgcccta
     6541 atcgcccgtg aactgggaat tggctggatt tgccagcagg gtgagaaact gtatgcgata
     6601 caaccagaag aaacgctaac gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa
     6661 accaccgttc ggatggcatc attctgatgt catccggcaa tttactctgc actgatgttt
     6721 tttaactacc tgttatattt ttcaccaaat tatgatgatt tttcttcgct aatcgcgaac
     6781 ccaacgtcgc tggctgacga ataaacagat gttattttat cactctattt cgccgttatt
     6841 tatcccttta attccctttt ctaaaatgtc tgtcagttcg cagaatgtga tttcgatcat
     6901 gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg aataccgttt tattcggttt
     6961 cgccaaagga gaatgattga tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt
     7021 aattccagcc tgcatctttt tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacatc
     7081 ggtaacacca caaccgcctg atattttatt agggccgctg tttaatgatg tgcaaaacgc
     7141 caaacttttt ccggaccaaa aaacctttgc cgatgccgtg ccgaacagcg atccgctgat
     7201 gatccttgct gattatcgga tgcagcaaaa ccagagcgga tttgatctac gccatttcgt
     7261 taacgtcaat ttcaccctgc cgaaagaggg cgagaaatat gttccgccag aggggcaatc
     7321 actgcgcgaa catattgacg gactttggcc ggtattaacg cgttctaccg aaaacaccga
     7381 aaaatgggat tctctattac cgctgccaaa gccttatgtc gtgccaggcg gacgttttcg
     7441 cgaggtatat tactgggaca gttacttcac catgttagga cttgccgaaa gcggtcactg
     7501 ggataaagtc gcggatatgg tggccaattt tgctcatgaa atagacactt acggtcatat
     7561 tcccaacggc aaccgcagtt actatttaag ccgctcgcaa ccgcccttct ttgccctgat
     7621 ggtagagtta ctggcgcagc atgaaggcga cgccgcgttg aaacaatacc tgccgcaaat
     7681 gcaaaaagaa tatgcttact ggatggacgg tgttgaaaac ctgcaagccg gacaacagga
     7741 aaaacgcgtt gtaaaacttc aggatggtac ccttctcaac cgctactggg acgatcgcga
     7801 tacgccacga ccagagtcat gggtggaaga tattgccact gccaaaagca atccgaatcg
     7861 acctgccact gaaatttacc gcgacctgcg ctctgccgct gcgtctggct gggatttcag
     7921 ctcgcgctgg atggacaatc cgcagcagtt aaacacctta cgcaccacca gcatcgtacc
     7981 ggtcgatctg aacagcttga tgttcaaaat ggaaaaaatc ctcgcccgcg ccagcaaagc
     8041 tatcggagat aacgcgatgg caaaccagta cgaaacgctg gcaaatgccc gtcaaaaagg
     8101 gatcgaaaaa tacctgtgga acgatcaaca aagctggtat gccgattacg acctgaaaag
     8161 tcataaagtg cgcaatcagt taaccgcggc cgccctgttc ccgctgtacg tcaatgcggc
     8221 agcgaaagat cgcgccagca aaatggcgac ggcgacgaaa acacatctgc tgcaaccagg
     8281 cggcctgaac accacgtcgg tgaaaagtgg gcaacaatgg gatgcgccaa acggctgggc
     8341 gccgttgcag tgggtcgcga cagaaggatt acaaaactac gggcaaaaag aggtagcgat
     8401 ggacattagc tggcacttcc tgaccaatgt tcagcacacc tatgaccggg agaaaaagct
     8461 ggtggaaaaa tatgatgtca gcaccaccgg aacagggggc ggcggtggcg aatatccatt
     8521 acaggatggc tttggctgga ccaatggcgt gacgctgaaa atgctggatt tgatctgccc
     8581 gaaagagcaa ccgtgtgaca atgttccggc gacgcgtcca ctcagtgaat caacaacaca
     8641 gccgctaaaa caaaaagagg cggaacctac gccttaactt tactgtatat cgagcaatgc
     8701 cattacgtgc ataaaattgg cattgctctt tcttcatcac gaaatacc
//
LOCUS       DAPQZD010000193         8596 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPQZD010000193 DAPQZD010000000
VERSION     DAPQZD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.195"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            139..927
                     /locus_tag="RO454_004327"
     CDS             139..927
                     /locus_tag="RO454_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDY0936066.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1133..1480
                     /locus_tag="RO454_004328"
     CDS             1133..1480
                     /locus_tag="RO454_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDY0936067.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1474..2313
                     /gene="sul1"
                     /locus_tag="RO454_004329"
     CDS             1474..2313
                     /gene="sul1"
                     /locus_tag="RO454_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDY0936068.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2441..2644
                     /locus_tag="RO454_004330"
     CDS             2441..2644
                     /locus_tag="RO454_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936069.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2800..4005
                     /gene="chrA"
                     /locus_tag="RO454_004331"
     CDS             2800..4005
                     /gene="chrA"
                     /locus_tag="RO454_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HDY0936070.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            4016..4321
                     /locus_tag="RO454_004332"
     CDS             4016..4321
                     /locus_tag="RO454_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0936071.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            4365..4430
                     /locus_tag="RO454_004333"
                     /pseudo
     CDS             4365..4430
                     /locus_tag="RO454_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4548..5312
                     /locus_tag="RO454_004334"
     CDS             4548..5312
                     /locus_tag="RO454_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDY0936072.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(5805..6389)
                     /locus_tag="RO454_004335"
     CDS             complement(5805..6389)
                     /locus_tag="RO454_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0936073.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(6389..7627)
                     /locus_tag="RO454_004336"
     CDS             complement(6389..7627)
                     /locus_tag="RO454_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936074.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(7624..8529)
                     /locus_tag="RO454_004337"
     CDS             complement(7624..8529)
                     /locus_tag="RO454_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDY0936075.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1714 a         2528 c         2592 g         1762 t
ORIGIN      
        1 aaggctaaca atgcgttgca gcaccagtcg cttcgctcct tggacagctt ttaagtcgcg
       61 tctttgtggt tttgctgcgc aaaagtattc cacaaagccg caacttaaaa gctgccgctg
      121 aacttaacgt taggcatcat gggtgaattt ttccctgcac aagttttcaa gcagctgtcc
      181 cacgctcgcg cggtgatcga gcgccatctg gctgcgacac tggacacaat ccacctgttc
      241 ggatctgcga tcgatggagg gctgaagccg gacagcgaca tagacttgct cgtgaccgtc
      301 agcgccgcac ctaacgattc gctccggcag gcgctaatgc tcgatttgct gaaagtctca
      361 tcaccgccag gcgatggcgg aacatggcga ccgctggagc taactgttgt cgctcgaagc
      421 gaagtagtgc cttggcgcta tccggcgcgg cgtgagcttc agttcggtga gtggctccgc
      481 cacgacatcc tttccggaac gttcgagcct gccgttctgg atcacgatct tgcgattttg
      541 ctgaccaagg cgaggcaaca cagccttgcg cttctaggcc catccgcagc cacgtttttc
      601 gagccggtgc cgaaggagca tttctccaag gcgcttttcg acactattgc ccagtggaat
      661 gcagagtcgg attggaaggg tgacgagcgg aacgtcgttc ttgctcttgc tcgcatttgg
      721 tacagcgctt caactggtct cattgctcct aaggacgttg ctgccgcatg ggtatcggag
      781 cgtttgcctg ccgagcatcg gcccctcatc tgcaaggcac gcgcggcgta cctgggtagc
      841 gaggacgacg acctagcaat gcgcgtcgaa gagacggccg cgttcgttcg atatgccaaa
      901 gcaacgattg agagaatctt gcgttgagcg gcatgtgcga aaagtgcatc gacccgcgcc
      961 gagggcatct gatgcctaac tcggcgttca agcggacggg ctgcgcccgc cgctcaacta
     1021 tgcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     1081 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     1141 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
     1201 tagcgagggc tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc
     1261 attttatttt ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg
     1321 gtcgggactc ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct
     1381 tgatgcgtgg ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc
     1441 cccatcgtgg aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat
     1501 ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc
     1561 gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc
     1621 catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta
     1681 gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag
     1741 cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct
     1801 gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca ctcagcgcag
     1861 cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct cgacgagatt
     1921 gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg
     1981 ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac atcgctgcac
     2041 gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg
     2101 tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct gggtccagcg
     2161 agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg cacccacgcg
     2221 cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt tcgcagtcgc
     2281 gacgccagag accgagggtt agatcatgcc tagcattcac cttccggccg cccgctagcg
     2341 gaccctggtc aggttccgcg aaggtgggcg cagacatgct gggctcgtca ggatcaaact
     2401 gcactatgag gcggcggttc ataccgcgcc aggggagcga atggacagcg aggagcctcc
     2461 gaacgttcgg gtcgcctgct cgggtgatat cgacgaggtt gtgcggctga tgcacgacgc
     2521 tgcggcgtgg atgtccgcca agggaacgcc cgcctgggac gtcgcgcgga tcgaccggac
     2581 attcgcggag accttcgtcc tgagatccga gctcctaggg atcgcctcag aaaacggaaa
     2641 ataaagcacg ctaagccgta agtaagcgtg ctcctgtgaa agccacagct aaaactgcgt
     2701 agtacacata gaggtatttt tttctagatt tatatcgaat ttcgttatcg aatttcgctt
     2761 attattgatc aactcgttgc agatcatgaa ggcgaaagta tgacgaataa ccctaccgat
     2821 gacagcagac catggtcggt ctttcttatt tttctgcggc ttggattgac atcttttggc
     2881 ggccccattg cgcacttggg ctacttccgc gccgaatttg tcacacggcg gcgctggctc
     2941 tccgaacgga gctatgctga cttggtcgcg ctttgtcagt tcttgccagg gcctgcaagc
     3001 agccaggtcg gcatagcggt aggactgtct cgggctggat acagcggggc gctggctgct
     3061 tgggctggct tcacgctgcc gtctgccata gccttgatcc tttttgcgct cggcatctcc
     3121 agctatggcg attacgtctc gcagggcgcg ttgcatggct taaaagtggt ggctgtggcc
     3181 gtggtcgctc aagcagtatg gggcatggcg cgtaacctat gcacggatgg gctgcgagtc
     3241 accatcatgg caattgctac ctgcgtcgtt ttacttgtgc cgtccgcgtg gggacaggtt
     3301 ggcgtgattg ctatcgcagg catcgcaggc cggttattgt tcaagccagc gaaagttgtt
     3361 gagcatgacc ccctacctat cacggtcagt caccgggccg gcgtgctttg gctctcgctg
     3421 ttctttgtct tgctgattgg cctgccggtg ttggccgaac tgatgccaag tcaaaccatg
     3481 gcaatggtgg attccttcta tcgtgtcgga tcactggtgt tcggcggtgg tcacgttgtg
     3541 ctgccattac tgcaagccga agtggtgccc tccggctggg tcaacaatga atcctttctc
     3601 gcggggtacg gggcagctca agcggtgccc ggccctttgt tcacgttcgc cgcgtttctt
     3661 ggtgcctcga tgaacaccgc cccgtcgggc tggatcggcg gcattgtgtg tctgctggct
     3721 atcttcgcgc cctcgttctt gctggtcgtc ggatcaatgc cattttggga gcgtttgcgc
     3781 cgcaatacag gcatccaagc tgcgctggcc gggatcaatg ccgctgtagt cggcttgctg
     3841 ctggccgcgc tgtatcagcc tgtatggact agcgccatct ttcagccgca agacttcggc
     3901 ttggcattag ttgcccttgt cgcacttatg ttctggaagc tcccgccgtg gctggttgtc
     3961 ctcggtagcg gtgcagctgg gtggctgttg agcgtcgctc tgtgaggcac aaaaaatgac
     4021 tgacaaagac ctctacggcg gtttgatccg cctgcacatc cttcaccatg cagccgagga
     4081 acctgtcttt gggctgggga tcatcgaaga gctacgccga cacggctacg agatgagcgc
     4141 tggcaccgtg tacccgatgc tgcacggcct ggaaaaaaaa ggctatctga cctcacgcca
     4201 cgaacgcacc gggcgacgtg aacggcgtgt ttatgacatc actgaacaag gcagaactgc
     4261 acttgctgat gcgaaaacaa aggtcaagga gctgttcgga gagttggtag aaggtggttg
     4321 agtttttgct tctatgtcag gttgagctat accctaactg gattgtcatt ttcagaagac
     4381 gactgcacca gttgattggg cgtaatggct gttgtgcagc cagctcctga cagttcaata
     4441 tcagaagtga tctgcaccaa tctcgactat gctcaatact cgtgtgggct ctgttgcaaa
     4501 aatcgtgaag cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca
     4561 agtggcgcca tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc
     4621 cgatcagcta tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata
     4681 cgacgatcta tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt
     4741 tctggcggcg tggctttgat ccgagctggc gcctggatga aacctacgtc aaggtgcggg
     4801 gcaagtggac ctacctgtac cgggcagtcg acaagcgggg cgacacgatc gatttctacc
     4861 tgtcgccgac ccgcagcgcc aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc
     4921 tgaagcactg ggaaaagcct gccacgctca ataccgacaa agcgccgagc tatggtgcag
     4981 cgatcaccga attgaagcgc gaaggaaagc tggaccggga gacggcccac cggcaggtga
     5041 agtatctcaa taacgtgatc gaggccgatc acggaaagct caagatactg atcaagccgg
     5101 tgcgcggttt caaatcgatc cccacggcct atgccacgat caagggattc gaagtcatgc
     5161 gagccctgcg caaaggacag gctcgcccct ggtgcctgca gcccggcatc aggggcgagg
     5221 tgcgccttgt ggagagagct tttggcattg ggccctcggc gctgacggag gccatgggca
     5281 tgctcaacca ccatttcgca gcagccgcct gatcggcgca gagcgacagc ctacctctga
     5341 ctgccgccaa tctttgcaac agagcccgcc gtgctagtct gctcggtgat ggtggagtga
     5401 agccaacccg caatcgggtt atgaatctgc atcgcgattc gcaatcagct gtctcttgag
     5461 catgtcgaac tcctgcgatg ttatctcgcc gtgctcgtgc tgctacacca acttgtccaa
     5521 ctcatccgcc acaccgctcc ccaccacctg cgatggccag gttgtttgct gggcgcattg
     5581 gcggagtatc gtctcgaccc gggacaccca caccggcaca accttacggg agtgattcac
     5641 tgtcaaagaa tcggcccggt gctctgacgc aagtatcggg atggtcacca tttgtaagcc
     5701 gtagacctga gtggtgatca agacttcgat accaccgacc gtaccggtac taatcgacga
     5761 cggtcgtgtt cgtcgcctgc cgcagggact ctgcacacct ccgtttacgc atgtgcctgg
     5821 aggagttgga aatcgtcgtg ttcgggaaac attaaacaca ggatggcagc gatctgagcc
     5881 agcacatgat cagctagctc accatccgga tcgacggccc actgcatcgt cgcgccagcg
     5941 atgaccgagt gcaggagcaa ctcagctgcc gcaggagcac ctgggggcag tcgcttgcgg
     6001 atcccctcca ccaccgcgcg gttccgctgg atcgcaagcg tgcgtagctc cggcacctgg
     6061 agctcgtacc aggagatgag atagttcacc gagaagtcgt tgcgagtgtt catgctccga
     6121 acgagcacct gcaaaaattc ccagagccct tgcggccctg cgcctatcgg tatcgcattc
     6181 aggtaatgcc gcacctgctc gacgccgcgc tccatcatcc tcaccagcag cgtatcgcgg
     6241 ttggtgaagc gctggattaa cgctgcgcgg gagagcccca cctcctttgc tactccgctg
     6301 agcgtgaact ctatgggacc gcaacgcttc agcactacgg tggcggcctc gagtacctcg
     6361 tcatcggact tgagcttggg gcggggcatc agtgttcacc ttctgtatgg gttggggcgg
     6421 aggctgtggc tgccgccgcc attgtagcaa attgaagacg gagcgagagt agagccacga
     6481 gccccgcaaa cacggccgat acaacgaggc cagggagcgg accagcaagg tcgacaaacg
     6541 cgccggccgc aagcataacc atgggcgagg ctgacagcat caccgccgag accgtgccga
     6601 gtacccggcc gagaagttct ggcggcgtgc ggttgtagat ggcagcgttg agaatgggag
     6661 agactgagcc ggtcagcagt cccacgagcg cgcccaacaa catcagcacc ggcacgcctg
     6721 gcaactgtga aagcagaagc gagcccaccg cagagccaca aaatgccacc gccagccagt
     6781 tctgcgctga tatccgggcg ccgaccgacg catgaatggc aatgccaagg agaccaccag
     6841 cccccatcat tgaggagaac agcccgagct ctgctacttg gcgtcctgca tctacaaaca
     6901 gcgcaggcat gatgacgctg ccgttggcgc caacgatgcc cacgaagatc atcactatac
     6961 caaagagagg gcgcagcagg ggttcgctcc agagaaaagc gacgccggcg cgcatggaga
     7021 gagtcgccgt cgtggtcatc gtccgagcgg cacgcgcggg aagcacccac gcgccgagca
     7081 gacctgcaag gacggagcag aacgccgtca gcccgagcgt tggcgcagcg ccaagcaggc
     7141 cgattgcggc ccccccaagg gccgggccac ctagaatcgc gacgttcccg atcaccgctt
     7201 tcagtgacga gacgcgctca acggagagcc cggcgacgtg gccgagtttg ggcagctcac
     7261 tgtcctgcgc ggccataccg ggtgcgtcga acgcggcacc gagcaccacg caagcgatca
     7321 gcccagtgtt cgagagggcg ccaacggcat cgagcagtgg gatgctcgcc atggccacgc
     7381 cgcccaccac acccgagatc aatgcgacgg gcgcgcgccc gaaccgatcg acgaggccac
     7441 caccaaccca cgcgccgatg atggtcgcga tgacgctgct agcggccgtg gcgcccgccc
     7501 aggccgcgct ctttgtatga gacaggacga accatggaag cgcgagggcc gccaccgcgt
     7561 tgccgatccg gaagagaaag gtcgccgcga acagcgtcgc gagcgggcta tatcgacgtt
     7621 cgctcattcc gctgcggcga gctgcgcctt cgccgcagcg aggtactctt cgttacccga
     7681 gtcgagggcg aagagtgcgt aggtgaccgc cccgaacgca aggcgctccg cgatgtggtg
     7741 ggcgagccgc ggccacaccc ggccaccggc cgcttcatac gtgaggagga gcttcgcgag
     7801 cccctcttca ccaaagacca taaggtgcgc ggccatgtcg atggcagggt catcaacgcg
     7861 ggcctcgctc cagtcgatca tcccgctgac gcgctccgtg ttgtcgatga gcacatggcc
     7921 cacgtagaga tcgccatgca ccaccacgga gaaatctggc cacgacgaat cgtcgtcgag
     7981 ccagcgctgc caccggtgga ggcgcttgtc gttcaccacg aactcgcgtc ggacgcggtc
     8041 aacgtcgtcg gccaccttct gacgggcctg cgtcggtgta cggatgagca tccccgcatc
     8101 cacggcggcg gaaatgggga cggcatgcag ggcggcgagc gcggtcgcga agctctccgc
     8161 gaagacctcc gagtcctgcg gcacgaccca gtcgggcgtg gacgaaccag gctggatgac
     8221 catcgcagtc gagtcttcga gcatgggata ggcaacgagc tcggcgttgg ccacgcgcca
     8281 gtccggcacc gcgaacggca ggcgattctt gagcattgcc agcacccgcg cctctggttc
     8341 gaccttcgcg cttacctcgg ctcggcgcgg gatgcgcagc acccaccgac gtccatcgtc
     8401 gacggtggcg atcacgatcc tatagtcgag cccaagctca ttgacagtca gcgggccatg
     8461 gagcttgagc ccatgtcggg ctgcaagtgc gtacagttgg gaggtatcgg cggtcgtgac
     8521 tacggtcatg attcactcct gagggcttga cgggtttagc cacctaaatg taacagtcac
     8581 gtcggttata ttcaat
//
LOCUS       DAPQZD010000194         8590 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPQZD010000194 DAPQZD010000000
VERSION     DAPQZD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.196"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            212..1333
                     /gene="ompC"
                     /locus_tag="RO454_004338"
     CDS             212..1333
                     /gene="ompC"
                     /locus_tag="RO454_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDY0936076.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1445..2500
                     /gene="apbE"
                     /locus_tag="RO454_004339"
     CDS             1445..2500
                     /gene="apbE"
                     /locus_tag="RO454_004339"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDY0936077.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2574..3638
                     /gene="ada"
                     /locus_tag="RO454_004340"
     CDS             2574..3638
                     /gene="ada"
                     /locus_tag="RO454_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDY0936078.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3638..4288
                     /gene="alkB"
                     /locus_tag="RO454_004341"
     CDS             3638..4288
                     /gene="alkB"
                     /locus_tag="RO454_004341"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDY0936079.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4364..6007
                     /gene="yojI"
                     /locus_tag="RO454_004342"
     CDS             4364..6007
                     /gene="yojI"
                     /locus_tag="RO454_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDY0936080.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6225..7871
                     /gene="mqo"
                     /locus_tag="RO454_004343"
     CDS             6225..7871
                     /gene="mqo"
                     /locus_tag="RO454_004343"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDY0936081.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8021..8509)
                     /gene="eco"
                     /locus_tag="RO454_004344"
     CDS             complement(8021..8509)
                     /gene="eco"
                     /locus_tag="RO454_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDY0936082.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2072 a         2134 c         2305 g         2079 t
ORIGIN      
        1 attccgcaat aatcttaaaa agttcccttg tatttacatt ttgaaacatc tatagcgata
       61 aatgaaacat cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg
      121 gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc ataaaaaagc
      181 aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt ccctcctggt
      241 cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa
      301 caaattagat ctgtacggta aagtagacgg cctgcactat ttctctgaca acaagtcaga
      361 agacggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca
      421 gctgaccggt tacggccagt gggaatatca gatccagggc aacagcgctg aaaacgaaaa
      481 caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtgg gttctttcga
      541 ctacggtcgt aactacggcg ttgtttatga cgtaacttcc tggaccgacg tactgccaga
      601 attcggtggc gacacttacg gttctgacaa cttcatgcag cagcgtggta acggcttcgc
      661 gacctatcgt aacaccgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta
      721 ccagggtaaa aacggtagcg tagatggcga aggcatgacc aacaacggtc gtggtgccct
      781 gcgtcagaac ggcgacggcg ttggcggatc tatcacttat gattacgaag gcttcggtat
      841 cggtgctgct gtttctagct ccaaacgtac tgatgatcag aacttcggcc tgaacggtta
      901 tggtgagcgt tatttgggta acggcgaccg tgctgaaacc tacactggtg gtctgaaata
      961 cgacgctaac aacatctacc tggctgctca gtatacccag acctacaacg caactcgcgt
     1021 aggttccttg ggttgggcaa acaaagcaca gaacttcgaa gctgttgctc agtaccagtt
     1081 cgacttcggt ctgcgtccgt ctgtagccta cctgcagtct aaaggtaaaa acctgggtgt
     1141 catcaatggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac
     1201 ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga
     1261 caaccgcttc acccgtgacg ctggcatcaa cactgatgac atcgtagctc tgggtctggt
     1321 ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt
     1381 tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
     1441 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg
     1501 ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
     1561 caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgacgcca aacgcagtgc
     1621 cgaacttaaa gaaaagattc agacccagct ggacgctgac gatcagctgc tttcgaccta
     1681 taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt
     1741 aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc attggcgcaa ggaccgatgg
     1801 tgcgatggat ataaccatcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca
     1861 accggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca
     1921 gcacctgacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
     1981 atatgtcgat ctctccaccg tcggcgaagg ttatgcggcg gatcacctgg cacgcttgat
     2041 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
     2101 tatgaacggt gcaggccaac cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa
     2161 cgcggttcag gccgtggtgg atatcaacgg tcatggtatt agcacctctg gcagctaccg
     2221 taactattac gaactggacg gcaaacgtct ttctcatgtt atcgatccgc aaaccgggcg
     2281 tcctatcgaa cacaatctgg tatccgtgac ggtgattgcc ccgacggcgc tggaggccga
     2341 tgcctgggat actggcttga tggtactcgg accggagaaa gccaaagagg ttgttcgccg
     2401 ggaaggactg gcggtctaca tgatcaccaa agagggcgat aattttaaag cctggatgtc
     2461 gccgcagttt aaaagcttcc ttatcagcga aaaaaattaa agcgcaagat tgttggtttt
     2521 tgtgcgatgg tgaccgggca gactaaaggc tatccttaac cagagagctg attatgaaat
     2581 acgccatacg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gactcgaatg
     2641 ccgacggcga attcgttttc gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc
     2701 gagccagaca tgccttgcgg gaaaatgtct ccttctacgc aaatgccagc gaggcgcttg
     2761 ccgctggctt tcgtccctgc aaacgttgtc agccagacaa agccaatccc ctgcaacatc
     2821 gccttgataa aattacccac gcctgccgac ttctggaaca ggaaacgcct gtaacgctgg
     2881 aagccttagc cgaccaggtg gcgatgagtc catttcattt gcatcggttg tttaaagcga
     2941 ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat
     3001 tgctggcgaa aggggagagc gtgactacat ctattcttaa cgccggattc cccgacagca
     3061 gcagttacta ccgcaaagcc gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg
     3121 gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggc cgttgcctgg
     3181 tggcagaaag tgagcggggg atttgcgcga tattactggg cgatgatgac gcgacactaa
     3241 tcagcgagtt acagcagatg tttcccgctg ccgacagcgc gcctgctgat ctgacgtttc
     3301 agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac
     3361 cgctggacat tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac
     3421 cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg
     3481 tacgggctgt tgccagcgcc tgtgccgcca acaagctagc tatcgttata ccctgtcatc
     3541 gggtagtacg cggtgatgga tcgctttcag gttaccgctg gggcgtgtcg cgtaaagcgc
     3601 aactgctacg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc
     3661 tgaaccgtgg caagaaccac tggcggctgg agcggtgatt ttgcaccgtt ttgcttttaa
     3721 cgctgcggag caactgatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca
     3781 gatggtcact ccaggcggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg
     3841 ctggacgacc catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc
     3901 gtggcccgcc atgccgcaga gttttcatca tttatgtcaa cgtgcggcta cggcagcggg
     3961 ctatccagat ttccagccag atgcctgttt gatcaaccgc tacgttcctg gcgcgaaact
     4021 gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct
     4081 gggcttaccc gcgatttttc aatttggcgg cctgaaacga aatgatccgc tcaaacgttt
     4141 gttgttggaa catggcgatg tggtggtatg gggtggtgag tcgcggctgt tttatcatgg
     4201 cattcaaccg ttgaaagtgg gggggcatcc actcaccacc gactgccgct acaacctgac
     4261 attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcacgaag
     4321 atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact
     4381 tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc
     4441 ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac
     4501 cagtctgctg gtattgccgg agtttctggg gttattgctg ctgttgatgg cagtcactct
     4561 cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga
     4621 atttatcaag cggattctgg atacccatgt cgagcgcatt gaacaacttg gtagcgcctc
     4681 gttgctggcg gggttaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc
     4741 ggaactggtg caggggatca ttctcactat cggttcagcg gcgtatttgt ggatgctgtc
     4801 gggcaaaatg ttgctggtaa cggctatctg gatggcagtc accatctggg gtggttttat
     4861 actggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag ataagctgta
     4921 cacggatttt caaaccgtac tcgaagggcg caaagagctg actctgaacc gtgaacgcgc
     4981 cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat
     5041 ccgcgcagac accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc
     5101 aatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgccgc
     5161 gacctattcg ttgacgttgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcgtt
     5221 gccgacgcta ctgacggcgc aggtggcgtt taataagctg aacaaattcg cgctcgcgcc
     5281 tttcaaagca gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg
     5341 taacgtgacg tttagctatc aggataacgc gttttccgtt ggtccaatta atctcaccat
     5401 taaacgtggc gagctgctgt ttctgattgg tggcaacggt agtggaaaat cgacgctggc
     5461 gatgttgctg acgggcttgt accagccaca aaatggcgaa atcttgctgg atggcaaacc
     5521 tgtcagcggc gaacaaccgg aagattatcg aaaactgttt tcggcagtgt ttaccgatgt
     5581 ctggctgttt gatcaactgc tggggccgga ggggcaaccc gctaacccgc aactggttga
     5641 gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt
     5701 taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga
     5761 agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg
     5821 tgagttttat caggtgctgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat
     5881 cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca
     5941 acttagcgag ctgacaggcg aagagcgcga tgctgcttcg cgtgatgccg ttgcccggac
     6001 agcataatat tacgacgctg acctggcgga ctgctgccgc caggtcaata ttctaaaaat
     6061 accttcgttt ctaataaccc tataattaat caacgaaatt ataatgtttc taaaattaga
     6121 atataattta taaacattat ttaaatgttg ttacttaagt gttaccgttg atgccgcgca
     6181 aatctcacct gcaataaagc gactaaaagt aaggcattaa caagatgaaa aaagtgactg
     6241 ccatgctctt ctcgatggcc gtggggctta atgccgtttc gatggcggca aaagcgaaag
     6301 cgtccgagga gcaggaaact gatgtactgt tgattggcgg cggcattatg agcgccacgt
     6361 tggggaccta tttacgcgag ctggagcctg aatggtcgat gaccatggtg gagcgcctgg
     6421 aaggtgtcgc gcaggagagt tcgaacggct ggaacaacgc cggaaccggg cattctgcac
     6481 tgatggaact caattacacc ccgcaaaatg ccgacggcag tatcagtatt gaaaaagcag
     6541 tcgccattaa cgaagcattt cagatttccc gccagttctg ggcgcaccag gtcgagcgtg
     6601 gtgtgctgcg tactccgcgt tcatttatca ataccgttcc gcatatgagc tttgtctggg
     6661 gcgaggataa cgttaatttc ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt
     6721 ttcgcggtat gcgttactct gaagatcacg cgcagatcaa agagtgggca ccgttagtga
     6781 tggaagggcg cgatccgcaa cagaaagtag cggcaacacg cacagaaatt ggcaccgatg
     6841 tgaactatgg cgagatcacc cgccagttga tcgcctcctt acagaagaaa tctaacttct
     6901 cgctgcaact cagcagcgaa gtccgcgccc taaagcgtaa tgacgataac agctggaccg
     6961 tcaccgttgc cgatctgaaa aacggcactg cacagaacat ccgcgccaaa tttgtcttta
     7021 tcggcgcggg cggtgcggcg ctgaaactgt tacaggaatc ggggattccg gaggcgaaag
     7081 actacgctgg tttcccggtg ggcggacagt tccttgtttc ggaaaacccg gacgtggtta
     7141 atcaccatct ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg atgtcggttc
     7201 cgcatatcga tacccgtgtt cttgacggta aacgcgtagt gctgtttggg ccatttgcca
     7261 ccttctcaac caaattcctc aaaaacggtt cattatggga tctgatgagt tccaccacca
     7321 cctctaacgt gatgccgatg atgcacgttg ggctggataa tttcgatctg gtgaaatatc
     7381 tggtgagtca ggtgatgttg agtgaagagg atcgttttga agtgttgaaa gagtactatc
     7441 cgcaggcgaa aaaagaggac tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca
     7501 agcgtgatgc cgataaaggt ggcgtactgc gtctcggtac tgaagtcgtc agtgaccagc
     7561 aagggaccat tgccgcgctc ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta
     7621 tgttgaatct gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta
     7681 cgttgaaagc gatcgttccg tcttatggac gcaagctgaa cggtgatgta gcggcaacag
     7741 aacgcgagtt gcagtacacc agcgaagtgc tgggtctgaa atacgacaag ccgcaggcag
     7801 cagatagtac gccgaaaccg cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata
     7861 ttgcgttgta atgatgtgcc acatccggca tggtttgccg gatgtggcgt atgctgataa
     7921 gacgcttcag cgtcgcatca ggcaaccggc tcgggcgtta gatgtcggat gcggcgtaac
     7981 cgccttattc ggcctacagg ttgcagcact tcaaagcagt ttagcgaaca accgcgttgt
     8041 caattttctc ttccgccttc cagacacggt acttcacatc cacattgtct ggcgtataca
     8101 ccacgatcgg cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga
     8161 caaatttctt ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa
     8221 ctttatcaaa gacataataa tcatagcccc agccttccag cgttttgctt tccagcttcc
     8281 cgccgagacg atgcaaattg cagtcgactt ccagcgtctg accgattaac agttctactt
     8341 tcagggtaga ttcatcttct tgcggagtta actgaatcac ctgacgcttc atgccttttt
     8401 cagcttgtgg ataaggcgcg attttttcca gtggctggac gctctctgcc gcccaggcgg
     8461 aagtggtggc gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt
     8521 ccttcttcat ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat
     8581 ttattaattt
//
LOCUS       DAPQZD010000195         8450 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPQZD010000195 DAPQZD010000000
VERSION     DAPQZD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.197"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..213)
                     /locus_tag="RO454_004345"
     CDS             complement(<1..213)
                     /locus_tag="RO454_004345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936083.1"
                     /translation="MSGNIGANPVPKLPLLAEWVDKNNDDLFVKAKSEKKERTVKKLK
                     NRGLATTLARINTWIDYDDDDNYKWVT"
     gene            complement(198..1426)
                     /locus_tag="RO454_004346"
     CDS             complement(join(198..1111,1111..1426))
                     /locus_tag="RO454_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0936084.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVLRAQLYVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            1517..2275
                     /locus_tag="RO454_004347"
                     /pseudo
     CDS             1517..2275
                     /locus_tag="RO454_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2433..3443
                     /locus_tag="RO454_004348"
     CDS             2433..3443
                     /locus_tag="RO454_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDY0936085.1"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGY
                     RPNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDE
                     HADCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDF
                     DNHAGTGQATQKLIALGHKRIALLSEDTSHTYVIARRQGWLDALNEHELEDTWLRLVS
                     PTRRAGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKAAVSLVVYDGL
                     PQDSIIELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHSP
                     CS"
     gene            3539..5665
                     /locus_tag="RO454_004349"
     CDS             3539..5665
                     /locus_tag="RO454_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDY0936086.1"
                     /translation="MVSKYCRLSSPRSDLIIKTHPHAEIIWWGSALKHFSPDDCASLE
                     RPVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDSSPVFYTVDVEHTENTLRLT
                     SEDSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFH
                     GRWTREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWS
                     GNHRMRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFK
                     GRNDDRAALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPD
                     WVLSMPGYSQPTGRYQYVLNLNIPEAFAYIYERFSWLLGEHPVDYVKWDMNRELVQAG
                     HEGRAAADAQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNND
                     ALERCTIQRGMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVA
                     ADAKESDGYRRYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQL
                     AMPDYTLPGILRFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGE
                     WLAQGGIQLPVLDPESAILIALERAV"
     gene            5728..7005
                     /locus_tag="RO454_004350"
     CDS             5728..7005
                     /locus_tag="RO454_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936087.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLL
                     RFNIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCATM
                     AGMLFNVDPSLVFWMGSGGALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFS
                     LFRMRQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNA
                     IIMFCTPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIA
                     LTVTVISAFTLSSSRGSVHPSVEKAPAAHSEIN"
     gene            7002..8435
                     /locus_tag="RO454_004351"
     CDS             7002..8435
                     /locus_tag="RO454_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDY0936088.1"
                     /translation="MMKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLI
                     YFNGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPVALAPGDKYDRDGCFSGSA
                     VDDDGVLSLIYTGHICLEDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFR
                     DPKVWHEEGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGESYMWECPD
                     FFRCGNFHWLMFSPQGMTPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDF
                     YAPQSMLAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREV
                     ESLRRKCQPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDN
                     QAGRLVLWRYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYP
                     QADSRQLSLYAAHGDAILTNGTLWMLT"
BASE COUNT         1918 a         2033 c         2144 g         2355 t
ORIGIN      
        1 tgtaacccac ttgtaattgt catcatcatc ataatcaatc cacgtattta tcctggctaa
       61 agttgttgct aggcctctgt tttttagttt ctttacagtc ctttcctttt tctcagattt
      121 ggcttttacg aataaatcat cattattttt atcaacccac tcagccagga gaggcaattt
      181 aggaactgga tttgccccta tatttccaga cacctgttat cacttaaccc attactggcc
      241 tgctgccgca gatattcccg tggcgagcga taacccagtg cactatgcgg atgccattcg
      301 ttataatgct cgaacgcctc tgcaaggttc tttgctgccg ttaacccgtc tggtttgggc
      361 atgatactga tgtagtcacg ctttatcgtt ttcacgaagc tctctgctat tccgttactc
      421 tccggactcc gcaccgccgt gttcttcggt tcaagtccca acatccgggc gaactggcgt
      481 gtttcattag cccggtagca tgaaccatta tccgtcagcc actccactgg agacgacgga
      541 agatcgttgc cgaagcggcg ttccaccgct cccagcatga cgtcctgtac tgtttcactg
      601 ttgaagccgc cggtagtgac cgcccagtgc agtgcctcac gatcacagca gtccagcgcg
      661 aacgtgacac gcagtctctc tccgttatca cagcagaact cgaacccgtc agagcaccat
      721 cgctgattgc tttctttcac ggccactctg cctgtatgtg cccgtttcga tggcggtaca
      781 gcaggttttc gctcaagcaa cagcgcattc tggcgcatga tccggtaaac acgtttggca
      841 ttgatcgcag gcataccatc aagttctgcc tgtctgcgaa gcagcgccca tacccgacga
      901 taaccatacg tgggcagctc tccgataaca tggtgtatac ggagaagcac atccgtatca
      961 tcagtgtgac gactgcggcg gccatccatc cagtcatcgg ttcgtctgag aatgacgtac
     1021 aactgcgcac gcaacacccg gagacaacgg ctgactaagc ttactcccca tccccgggca
     1081 ataagggcgc gtgcgctatc cacttttttg cacgcccgta ttcaacggct tctttaagga
     1141 gttcattttc catcgttttt ttgccgagca ggcgctggag ttctttaatc tgcttcatgg
     1201 cagcagcaag ttcagaggca ggaacgacct gctctcctgc ggccacagca gtaagacttc
     1261 cctcctggta ttgcttgcgc cagagaaata actggctggc tgccacaccg tgttgccggg
     1321 caacaaggga gaccgtcatt cccggttcaa aactctgctg aacaattgcg atcttttcct
     1381 gtgtagtacg ccgtctgcgt ttctccggtc ctaagacatc aatcatctgc tctccaatga
     1441 ctagtctaaa aactagtatt aagactatca cttatttaag tgatattggt tgtctggaga
     1501 ttcagggggc cagtctatcc tcccgctgga aatgctggaa attctgcgcc gcatagaaaa
     1561 acgtggctcg ctggaaaaac tgcgtaaggt ccgccaatac tgtaatcaga tttttcgtta
     1621 tgctatcgcc accggacgag ccactgtcaa tccggcatct gaactgacca gtacgctggc
     1681 ggcaccaaaa gctgcgcatt tcccccacct gagagcagat gagctccctg tttttctccg
     1741 gaagctcgct gaatatcatg gcagtcctgt tacccgcatg gcgacaaatc ttctgcttct
     1801 gacgggtctc agaaccattg agctacggtc cgctgaatgg tcagaaattg attttgataa
     1861 tgccctgtgg acaatccctg aaagccgcat gaaaatgcga cgtaaacatg tcgtaccact
     1921 gtcacgacag gccactgaca ttctgctgca gctcaaaact ttctccgagc aataccgact
     1981 ggtttttccg ggacgttgtg atatcaacaa gccaatgagc gaagccagca tcaatatggt
     2041 gctcaaacgt atcggttacg atggcagggc aaccggtcat ggttttcgtc acaccatgag
     2101 taccattctg cacgaacagg gctttaattc tgcctggatt gaaatgcagt tagctcatgt
     2161 ggataaaaat tccatcaggg gtacctataa tcatgccctg tatctcgatg gtcgccgtga
     2221 aatgatgcaa tggtacgctg actacattga ttcactttcc agccaggaga gttaattaca
     2281 ctttgtcctg aaattattct cacaatcaat aacctttccc tctgatctta cagatcagag
     2341 ggattccgtt aacttacaat aagtgatatt ggttgtctgg agattcaggg ggccagtcta
     2401 acggtctact ataataatta gcaggagata atatgtcact taaggcgatt gccactgcac
     2461 tcggagtttc tgtcaccact gtcagtcggg ctcttggagg ctatgcagat gtgtcagctt
     2521 ccacccgtgc acgggtggaa gctgaagctc gtcgacgcgg ctaccgtcct aatactcagg
     2581 caagaagact caaaaccggt aaaaccgatg ctgtcggtct ggtttatcct ggacgtgatg
     2641 tgccatttaa cagtggtgtt tttatggata tggtcagttg catcagccgg gaacttgctc
     2701 accatgatat tgacttacta ctgatagctg atgatgagca tgccgattgc cacagctata
     2761 tgcggcttgt tgaaagtcgc agggttgatg ctctcatcat tgcacacact ctggatgacg
     2821 atccccgtat cacacacctt cataaagcag gtattccatt tctggctctt ggtcgggtac
     2881 catctggtct gccctgtgcg tggtttgact ttgataatca tgccgggact ggacaggcaa
     2941 cccagaaact gattgctttg ggacataaac gtattgcatt gttgagtgaa gacacttcac
     3001 atacctatgt tattgcaaga cgtcagggat ggcttgatgc actgaatgag catgaactgg
     3061 aagatacatg gctgcggctg gtttctccca cacggcgagc aggatatcag gccgtgatgg
     3121 agttaatgtc actaccgata ccaccaacag ctattattac tgacaatgat ctgagtggag
     3181 atggtgctgc catggcgctg cagttgagcg gtcgtctttc aggaaaagct gcggtatctc
     3241 tggttgtata tgatggtttg ccacaggaca gtattattga gctggatgta gctgcagtta
     3301 ttcagtcaac acgaagcctc gttggtcgcc agatttccga gatgatatat cagataatta
     3361 ctgattcatc atcaaaacca ctacaggttg tctggaaccc ggtattttat ccgggaaaga
     3421 cgattcattc tccttgctcc tgattttttt atccagctca catatccgaa acgttttggt
     3481 tgatacccga aacgtttcgg atcaacagta aggcatccct gagaaaggag aatgtcttat
     3541 ggtttcaaag tactgcagac tgagcagtcc tcgctctgat ttaattatta aaacccatcc
     3601 acatgcagaa attatctggt ggggctctgc actgaaacat ttctcaccgg atgactgtgc
     3661 cagcctggaa agaccggttg cgaatggtcg tctggatatt gatacgccac tgacactgat
     3721 ggctgaaaat gccctgggac tttttagctc tccgggactg gaaggacaca ggaatgggct
     3781 ggattcatct cctgtttttt atacagttga cgtggaacat accgaaaaca ctctgagact
     3841 taccagtgaa gattcggtag ccggcctgcg tctggtcagc gagctggtga tgacgccatc
     3901 aggaattctg aaagttcgtc atgcactgac caacctcaga gagggggact ggcagataaa
     3961 tcgtttcgcc atcactttac ctttagcgga gcgtgcggaa gaagtcatgg cttttcacgg
     4021 acgctggact cgtgaatttc agccgcacag ggtacgtctc actcatgatg cttttgttct
     4081 ggaaaatcgc agagggcgga catctcatga gcattttccg gcgctgattg tcggcacgcc
     4141 aggcttctcg gaacaacagg gagaggtatg ggctgtgcat ctggggtgga gtggaaatca
     4201 ccgtatgaga tgtgaggcaa aaactgacgg caggcgttac gttcaggctg aggctctgtg
     4261 gatgccgggt gagaaggctc tcaggaagaa tgaaaccctg tacaccccgt ggctatatgc
     4321 ctgccactct gcggatggcc tgaatggaat gagtcagcaa taccatcgtt ttttgcgtga
     4381 tgaaattatc cgtttccctg agcaaaaacc ccgccccgta catctcaata cctgggaagg
     4441 tatttatttc aatcacaatc ctgattacat catgcagatg gctgagcgtg cagcagcact
     4501 gggcgttgaa cgtttcatta ttgatgatgg ctggtttaaa ggacgtaacg atgaccgcgc
     4561 ggctctgggc gactggtata ccgatgaaca gaaatacccg aacgggctga tgccggttat
     4621 taaacatgtg aaatctctcg gtatggaatt tggcatatgg gttgagccgg aaatgattaa
     4681 cccggattct gacctgtttc gtcttcatcc tgactgggta ttgtcaatgc ctggatattc
     4741 ccagccaacc ggaagatatc agtatgttct taacctgaat attcctgagg cctttgctta
     4801 catttatgaa cgtttctcat ggttactggg agaacatccg gttgattatg tgaaatggga
     4861 catgaatcgt gagcttgtac aggcagggca tgaaggccgt gcggcagcag atgcacagac
     4921 ccgtcagttc tatcgattgc ttgatctcct ccgtgaacgt tttccacatg ttgagtttga
     4981 gtcctgtgct tccggtgggg ggcgtattga cttcgaagtc ctgaaacgca cacaccggtt
     5041 ctgggcatct gacaataatg atgccctgga gcgctgcacc atacaacgtg gcatgagtta
     5101 ctttttccct cctgaggtga tgggggcaca tattggccat cgccgctgcc atgcaacttt
     5161 ccgacagcac agcatcgctt ttcgtgggct gacagcattg ttcggccata tggggctgga
     5221 gctggatccg gtggccgcag atgcgaagga atctgacggt tatcgccggt atgccttgct
     5281 ctataaagaa tggcgacaac tgattcatac aggtgttctc tggcgtgtgg atatgccaga
     5341 tccttcgata caggttcagg gagtcgtcag ccctgatcag tctcaggcac tttttatgat
     5401 cagccagctt gcaatgccgg attacacctt accaggcata cttcgttttc ccggtctggc
     5461 ggcggaagtg cgttaccggc ttcgggttat tgatcacccg gacctccagg tggtcggtga
     5521 aggcggtcat accatgcgca aattacctgt ctggatgaat cagagccttg aggccagtgg
     5581 tgaatggctg gcacagggag ggattcagct ccccgtactg gatccggaga gtgcgatttt
     5641 gatagcactt gaaagagctg tgtgaagaga ggtacggagc ctcagctccg tcctgtttct
     5701 caatattatc ccgtgagggt aagaataatg aattctgcaa gtacgcataa aaatcccgat
     5761 ttctggattt tcggtctgtt cttctttctc tactttttca tcatggcaac ctgttttccg
     5821 tttttgcctg tatggctgtc cgatgtggtc gggctgagta aaacggatac aggtatagtc
     5881 ttttcatgtc tttctctgtt tgccatcagt ttccagccat tgcttggggt catatcagat
     5941 cgcctgggac tgaaaaaaaa cctgatctgg agtatcagcc tgttactggt atttttcgcc
     6001 cccttttttt tatatgtatt tgcgcccctg ctgcgcttca atatctgggc aggcgcactg
     6061 actggcggtg tttttattgg ttttgtgttt tctgcaggtg ccggagctat tgaagcttat
     6121 atagagcggg tcagtcgcag tcgtggattt gaatacggta aagcgaggat gttcgggtgt
     6181 ctgggctggg cgttatgtgc gactatggct ggaatgcttt ttaatgtcga tccttctctg
     6241 gttttctgga tggggtcagg aggcgcatta ttgttgcttc ttctgttgtt tctggcgcgc
     6301 cccagtacca gccagacggc aatggttatg aatacactgg gtgccaattc ttccctgatt
     6361 tcgaccagaa tggtcttcag cctgtttcgc atgcgtcaga tgtggatgtt tgttctctac
     6421 acgattggtg tggcctgtgt ctatgatgta tttgatcagc agtttgccac attttttcgt
     6481 tcattctttg acactcctca ggcaggaata aaagcattcg gatttgctac cactgcgggg
     6541 gagatttgta atgccattat catgttctgt acaccatgga taattcatcg cattggtgcc
     6601 aaaaataccc tgcttgttgc ggggggaatt atgaccatcc gcattaccgg ttctgctttt
     6661 gccaccaccg cgacagaagt ggtgattctg aaaatgcttc acgctcttga agttccattt
     6721 ttgctggttg gggcgttcaa atatattacg gcagtgtttg acacccgact gtcagcgacc
     6781 gtttatttaa taggttttca gttttccaaa caacttgctg caatacttct ctctaccttt
     6841 gccggccacc tgtatgatcg tatgggattc cagaatacgt attttgtgct cgggatgatt
     6901 gctctgactg ttaccgtgat atcagctttc acgctgagtt cttcccgcgg gagcgtacac
     6961 ccttctgtag aaaaagcccc tgcagcgcat tcggagatta actgatgaaa cagcgtcttg
     7021 ctttggcaca gtccgccctt gaaaaacttt gcgcacgtcg tggtaatgcc tggtacccga
     7081 tttttcatct ggctccacct gccggctgga tgaatgatcc aaatggcctt atttacttca
     7141 atgggcgtta ccatgcgttc ttccagcatc atcctgcaag cgcatatcag gggccaatgc
     7201 actgggggca tgccaccagt accgacatgt tgcactggca acacgaacct gtcgcgctgg
     7261 cacccggaga taaatatgat cgtgatggct gtttttcagg gagtgccgtg gatgatgatg
     7321 gcgtgctatc acttatttat actggtcata tttgtctcga agatcgtggt aatgacagca
     7381 ttatccgtga agtacagtgt ctggctacca gtcatgatgg tattcacttt gagaagcagg
     7441 gctgtgtgct gacacctccg gaaggtatca tgcatttccg tgatcccaaa gtctggcacg
     7501 aagaaggctc ctggtggatg gtcattggtg cccgggacgc ttctgacaat gggcaagttc
     7561 tgttgtatcg cgggacatct ttgcgggact ggcatctgga gcatgttctc gctcattccg
     7621 cagccggaga aagttatatg tgggaatgcc ccgatttctt caggtgtggt aattttcact
     7681 ggctgatgtt ctcaccacag gggatgaccc cttccggtta tcggttccgt aacctttttc
     7741 agagcggtgt gttggcaggg agctggaagc ctggttctgt ctttgcgctg aaagggggat
     7801 ttgaagagct ggattatggt catgactttt atgctccaca gtccatgctg gctgaggacg
     7861 gcaggcgtat cattatggca tggatgaata tgtgggattc acccgtgccc acccgcagtg
     7921 aagcctgggc aggatgtctg acgctgccca gagaggtttt tgagcgcgat ggccggctgt
     7981 gccagcgacc tgtgcgtgaa gtcgaatctc tgcgcagaaa atgccagcca ttatcccctg
     8041 taaggttaca gggtttgcaa ttactgaccg aaaatgtaca ggccgcagaa ttattggtga
     8101 cgtggcatac ggttgacagt catgcggagc actatggcgt ccgccttgga gacggtctgc
     8161 gactttatgt ggataatcag gccggacgac tggtactgtg gcgctattac cctgaggaag
     8221 ggctggatgg ttaccgcagt gttgaacttc ctgatacaga atatctgact cttcgtattt
     8281 tcctggatcg ttcatctgtt gaagtgtttg ttaacgatgg tgaggcaacc ttatcaagtc
     8341 gtatttatcc gcaagcggac tcgagacaat tatcgttata tgccgctcat ggcgatgcga
     8401 tattaactaa tggcacttta tggatgctga cctgagatat atcgatatca
//
LOCUS       DAPQZD010000196         8342 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPQZD010000196 DAPQZD010000000
VERSION     DAPQZD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.198"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            167..502
                     /locus_tag="RO454_004352"
     CDS             167..502
                     /locus_tag="RO454_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDY0936089.1"
                     /translation="MAILITQDRQVANLTPPEGKSRITVSVKSKVGSGLSVESRSGSQ
                     TKSWIYRPYLNGKQIKITLGSYPAMTLSQAREAHADALALVKQGIDPRYARKTEKQQN
                     EQMPIFSQL"
     gene            710..1486
                     /locus_tag="RO454_004353"
     CDS             710..1486
                     /locus_tag="RO454_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDY0936090.1"
                     /translation="MTLDHAALQGLIDHNPARLLKPAMFGASMGKPRERWLPQDELQM
                     LWKALDEAGFGGGSVATGGRGIASSVILSQAIANALRLIILTGVRRSEAVNMRWEQIN
                     GNRWTIPETKNGKSHIVTLHPLALSLLKTQRIISEGGWVFESLSKPSFPVTGDAITRA
                     LERLKTKYMAEVAPFSPHDLRRSIATGCAEYLDAPERLIELLLNHVPKDRLIRTYQVG
                     QQAEKLRNLFLKWGDFIEHEIIESDNGTPDNVIQISFGKR"
     gene            complement(1651..2571)
                     /locus_tag="RO454_004354"
     CDS             complement(1651..2571)
                     /locus_tag="RO454_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDY0936091.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAMLKDKNPAKYVAAVNKLERVLE"
     gene            complement(2558..3340)
                     /locus_tag="RO454_004355"
     CDS             complement(2558..3340)
                     /locus_tag="RO454_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDY0936092.1"
                     /translation="MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGKPQVWLAL
                     PAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
                     CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYL
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRTFKPEGVEDGSA"
     gene            3624..6722
                     /locus_tag="RO454_004356"
     CDS             3624..6722
                     /locus_tag="RO454_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDY0936093.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYHYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENGDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSEETEIAVLKDNNLEVIRTNPEFQTNKDHNRPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKYIYAHPSFVKPMLNYIVTDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTAADEVSEEMPVLQAAEPAAVYHLHAKETRKIKRAALILHDIGS
                     KQERKEWVEQFKAGKIDLLFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRT
                     YNHFRYGYVVDFADIRQEFDATNKAYFEELQAELGDEMEHYSRLFKSAEEIREEIEHI
                     KDVLFSFDTENAEIFTDQINQIQDRETALALKNALADARSLYNLIRLQGNTEFLQALD
                     FNKLNILYREASRRLDLLNLKADLEQGVDLSGLLNRALEEVIFEFHKVGEEELVLADE
                     LKETLRRTREALAANFDQQDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIH
                     GRIKELNRQNNLLRQKYRGDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKV
                     LNNSQILNNESYFERHMESCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGAR
                     TW"
     gene            6716..>8342
                     /locus_tag="RO454_004357"
     CDS             6716..>8342
                     /locus_tag="RO454_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDY0936094.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGGALKRFDYIVSNPPFKMDFSDFRDALDSKENQQR
                     FFAGIPKIKAKARDKMEIYQLFLQHIIFSLKPGGKAAVVVPTGFITAQSGIDKGIREH
                     LVQNKMLAGVVSMPSNIFATTGTNVSILFIDASNKEKVVLIDASNLGEKVKDGKNQKT
                     VLTECEEKRICEAFNNKWSEEDFSVVVSYDDIAAKNYSFSAGQYFDVKIEYTDMTPEQ
                     FAAKMKGFTENLNNLFEQSRELEVQIKMQMADIK"
BASE COUNT         2347 a         2275 c         1980 g         1740 t
ORIGIN      
        1 ccaaaaagca tctatttttc cgcatatcta cacgtctcta taaatctcat aaaaatatta
       61 aaaatcatat aattaaacaa aaaacccatc ttatattgtt ccatcgaatt tcgctctata
      121 caatgctaaa caagtacgcc agcaagtacg cgagatgagg ccaaaaatgg caatattgat
      181 aacccaggac aggcaagtcg caaacctgac tccccctgaa ggaaaaagtc ggataaccgt
      241 cagcgtcaaa tcaaaagtag gttccggcct gtctgtcgaa tcccgttccg ggagtcaaac
      301 gaagagctgg atttaccgcc cttacctcaa cggaaaacag attaaaataa cgttaggctc
      361 ctaccctgca atgacattgt ctcaggcgcg tgaagcacat gcagacgcgc tggcgctggt
      421 taaacaaggt attgatccgc gttatgcgcg aaaaacggaa aaacagcaaa atgaacaaat
      481 gccgattttt tcacaattat gaaaaaactg gctggcattc cgtgaaacca ataaacccat
      541 tggggcgcgt actctggctg attatgaggg aacatatcgc cgccatctgg agaaaggttt
      601 aggttcagta cgcgtctgcg acctgtccag agcctacatt tatgaacatc tcatacggat
      661 cagaaagacc agcgccgaag gtgtccgcaa gggacttatt cttctcaata tgacgcttga
      721 ccatgccgct ttgcagggat tgatcgatca caaccctgct cgcttgctta aacccgcaat
      781 gtttggtgcc tcaatgggaa aaccgcgtga gcgttggttg ccacaagacg agttgcaaat
      841 gttatggaag gcactggatg aagcggggtt cggcggcgga tctgtcgcaa ccggagggcg
      901 aggcatcgcg tccagcgtta ttctttcaca agcgattgct aacgcattac gcctcatcat
      961 cctgacgggg gtgcgacgtt ccgaagcggt gaatatgcgt tgggagcaga ttaatggcaa
     1021 ccgctggaca atacctgaaa ccaaaaacgg taaaagtcat atcgtgacgc tacatcctct
     1081 ggcgctttct ctattgaaga ctcagcgcat tatttcagaa ggcggctggg tattcgagtc
     1141 gctcagtaag ccaagttttc ctgtcaccgg tgacgcaata actcgtgctc ttgaacgcct
     1201 gaaaacaaaa tatatggcag aagtcgctcc tttctcacca catgatttac gccgcagcat
     1261 tgctacaggc tgcgctgaat atctggacgc accagaacgg cttatagaac ttctgttaaa
     1321 tcatgtgccc aaagacaggt taatcagaac ctaccaggtc gggcagcaag cggaaaaatt
     1381 acggaatctg tttctgaaat ggggggattt tattgagcat gagatcattg agtctgataa
     1441 tggtactcca gataatgtta ttcagatcag ctttggaaag agatagtttc gtattttcat
     1501 gagtagagat cgtctgataa ctcagcgatc tcttactcga tggtgctatg tattgaatta
     1561 aatgaatatg tttgagttat tgccacgaag ttattcatat aaccaaagca tcatggccca
     1621 attcagagtc cctgaacagc gctaagaata ttactccagc accctctcta gcttattcac
     1681 cgccgccaca tacttcgccg gatttttatc tttaagcatc gccagattgc gttgcttcca
     1741 gcgaatagcc ggaagatgct gaatttcagg atagcgatac cgcgaccagt cagggctgtt
     1801 ttgcttaaaa ctgagcaaga actcgcgatc cagatccgta aaatgtgact tcaacgcctg
     1861 cggcagcaaa gtcgttactg acagaagcga ttccagcgac gtttcctgct gagtcatccc
     1921 cgaaaattcc tgagcgtata acgtggcaat ccgatctgta tcccagtttg aagccagcaa
     1981 ctcggcaatg ggacggggat ggcctgccag ataacaaaga aaaccgtcaa aaatttcgcg
     2041 cgtcaggccc ggtctcccca gcatattcaa cacatcaaac agatcgcgcg gatgctgacg
     2101 atcaagcgca gcacatattt taccgccata aagatccgcc agcgagacga cactcatggt
     2161 ggtgaagcca tactccattt ccacacgttc gcacacaggc atttctgccg gaggcaacaa
     2221 cagcccgcgc cagaccgggc taacctcgat cttaatcttt gcatcagcgg tattcacaat
     2281 gatgcgcttt tcatccgctt tattttcctg ccgaatcgcg gtgatccccg gtctgccgtt
     2341 cagcgatgca gtaattctct tcaaagcgtc gttgatcgcc gccagatcgg tatcgcgatc
     2401 cgcaaagctc ttatacgcca gatcgatatc caccgataga cgggggaaat cctgcacaaa
     2461 taaattgatt gctgtgccgc ctttcaacgc gaaacaggat tccttcgcca catgcggcag
     2521 agcggccatc aacagtttaa cctgcctggc atagctatca cgcagatcca tcttcgactc
     2581 cttccggttt aaatgtgcgg ggaacagtaa tttgataatc cggctccagc cagccaccag
     2641 gtatgatttg ccgctttccc gtacctgttt ctataccgct ttgatcaaga tactgaaaat
     2701 aagggtgtcc attacgccgg gcaaggtaaa gcatcacccg gttggtttta atcgaatgac
     2761 tggcgcgaag cagtgcctgc aatttgcgtg ggctgagaag attccccccc tgaaacagag
     2821 catcggcatg ttcgaacgag atcaacgaag gaacgccgca ggcgatctca tacgcggcca
     2881 gttccggtgc actgcctgct aactgccggt cgccaatttt cagttccgta gtaaatgacg
     2941 agacatcaac ggtaaggccc gatgtataca gcgtgataaa tgttgctgat tgttcgaagg
     3001 cagcaaacca tcccggcagt ttgacgtaag ccggaagcgc gagccagacc tgcggcttac
     3061 ccggttcggt atagtgcgca tggccctgaa gcgcaaggct ggttagcccc gccacccgaa
     3121 caggcttttc ccactgtgtt tgaaggcagt aaaccgcgtc tacccaatcc ggctcacggc
     3181 ccgtccggca gtacacaccg tgagcgatgc gcctgagcca gccattctga acatagagat
     3241 aagaaagctt acgatctacg ccattgcggg taagccatga ctggagcagc acctgaccgg
     3301 gtagcgtgtt ttgcatcaac cagtttagat agtttggcaa atgtcgctcc tgaatagaca
     3361 ttagtagaaa tatttaaacc agtgtaggtt cagggatacg ccacagcaag aaatagttta
     3421 atgattcctc atttcgtcca cttaaaatcg acattgagca aaaaatacaa acttacctac
     3481 attcaggcaa agtgcgacaa atagactcgg tcggtttaaa gagaacgtgt aacatagatg
     3541 caaatcgaac agacattgtt gccagccgtt ccaaggttgt ggggcgctgc atggcctgag
     3601 acgtgataaa tacagggaac acaatgaaat tcactgaaga tacccgagta aaaatccccg
     3661 tcatcctcca cctgatacgg ctgggctacc actacctgtc ccttaaagaa caacgctggg
     3721 acaaagaaac caatatcttc cctgacctct tcaccgctgc gattggcaga ataaatcccg
     3781 gacttgcgcc agacgacatc ggacgcctgt taaaagacct cacgctgctt ctcgacaacg
     3841 atgatctggg ccgcgccttc ttcgaaaaac tcagtgaccg ttccggtatc aaactgatcg
     3901 actttgataa cttcaacaac aacagtttcc atgtcgtcac cgaactgaca tgcgaaaacg
     3961 gtgatgaaga attccgcccg gacatcacgc tgctgatcaa cggcatgccg ctaatcttta
     4021 tcgaagtgaa aaaacccaac aatcgggaag gcatcgtcgc agaacgcaac cgcatcaacg
     4081 aacgctttaa aaaccccaaa ttcaaacgct tcgccaacat cacccagttc atgatcttct
     4141 ccaataacat ggagtatgac gacagcgacc cggagccgat tcaaggggca ttctacgcca
     4201 gcagctctta cagcaacccg gtattcaact acttccgcga agaagaactc ttcaacctga
     4261 ccaacttact gaaaccgctg tcagaagaaa ccgaaatcgc cgtcctgaaa gacaacaacc
     4321 tggaagtgat ccgcaccaac ccggaatttc agaccaacaa agatcacaac cgcccgacaa
     4381 accgcctctg caccgcgctg ttgagccggg atcggctggc atttatcctg cgctatgcgc
     4441 tggtgtatgt agccgaaccg ggcggcatcc aaaaacacat catgcgctac ccgcagctct
     4501 tcgccaccaa agcgattgag cgcaagctga acgcgggtgt gaaaaaaggc attatctggc
     4561 acacccaggg cagtgggaaa accgcattgg catactacaa cgtgcgcttc ctgacagatt
     4621 acttccagaa tcagcaggtg atcccgaaat tctactttat tgtcgatcgt cttgatctgc
     4681 tccagcaggc gcagcgtgaa ttcaccgccc gtggcttaat cgttcacctt atcgactccc
     4741 gcgaagcctt cactcgcgat atcaaagcaa cgcaggcgct gcacaaccac cacggcaaag
     4801 cggaaatcac cgtcgtcaac atccagaaat tccgcgatga cccggatgtc gtcaccacca
     4861 aagactacga catcaacatc caacgcgtgt acttcctcga tgaagtccac cgcagctaca
     4921 accccaaagg gagcttcctg gcgaatttaa gccagtcgga caccaacgcc atcaaaattg
     4981 gcctgaccgg tacgcctctg ctgggcacag actacaactc ccgcgacctc ttcggcgact
     5041 acatccacaa atactactac aacgcctcca tcgccgacgg ctacacgctg cgcctgatcc
     5101 gcgaagagat cgccaccaac taccgaatga tcctgcaaca ggcgctaaaa gaggtggaag
     5161 tcagaatggg cgatgtggat cgcaaataca tctacgccca tcccagcttt gttaaaccga
     5221 tgctgaacta catcgtcacc gactttgcca aaagccgggg cgcgctgaat gatgacacca
     5281 tcggcggcat ggtgatttgc gacagctctg aacaggccaa gcagatgttt gagatattca
     5341 acactgacta cgccgaatcg cccaccgccg ccgatgaagt ctccgaagaa atgccggtat
     5401 tacaggccgc agaacctgcc gccgtttacc acctccacgc caaagaaacg cggaaaatca
     5461 aacgcgccgc cctgatcttg cacgacatcg gcagcaagca ggagcgcaaa gaatgggtgg
     5521 agcaattcaa agcgggcaag attgacctgc tgttcgttta caacatgttg ctcaccggct
     5581 tcgacgccaa acggctgaaa aaactctacc tcgggcgggt gatcagaaag cacaacctgc
     5641 tccaggcgct gacccgcgtc aaccgtacgt acaaccactt ccgctacggc tacgtggtgg
     5701 acttcgccga catccgccag gaatttgacg ctaccaacaa agcctacttc gaagagctac
     5761 aggctgaact tggcgacgaa atggagcact actcccgcct gttcaaatcg gcggaagaga
     5821 tccgcgaaga aatcgaacac atcaaagacg ttctgtttag cttcgatacc gaaaacgcgg
     5881 aaatcttcac tgaccagata aaccagatcc aggaccggga aaccgcgctg gcgctaaaaa
     5941 acgcgctggc cgacgcccgc agcctgtaca acctcatccg cctccagggc aacactgagt
     6001 ttctccaggc gctggatttc aacaaactca acatccttta tcgcgaagcc agccgccgcc
     6061 tcgacttact caacctaaaa gcggatctgg aacagggcgt tgacctctcc ggcctgctga
     6121 accgggcgct ggaagaggtg atctttgaat tccataaagt gggtgaagaa gagctggtac
     6181 tggccgacga gctgaaagag acgctgcgcc gcacccgcga agcgctggcc gccaacttcg
     6241 accagcagga cccgcagttc gtcagcctga aagacgaact ggagcgtctg tttaagaaaa
     6301 agaaactcag cgaagtcacc cagcaagaaa tggttgcgaa tatcggcaca ctcaataaaa
     6361 ttcatggccg catcaaagag ctaaaccgcc aaaacaacct gctgcgccag aaataccggg
     6421 gcgacgtgaa atacgcgcgt acccacaaac gcctgcgcga gcgcggcggc ctgagcgagc
     6481 cggagcgtaa aatcttcgaa gcactgctta gcgttaagct ggatgcagat gaaaaagtgc
     6541 tgaacaacag ccagatactg aacaatgaaa gctattttga gcggcatatg gagtcatgcg
     6601 tcatcacgca ctttgaggat gagcaccata tcactctgac ctccagtgcg gtaagcgaca
     6661 tcaaccgcct gattgtggcg gaatacctga acgaatttaa taccggagca cgaacgtggt
     6721 agaactggaa tttagagaca aaaccaaagc cctgatcgac agcctgaaaa gcatctgtgc
     6781 gaactacggc ctgggcaacg acggtaacga attcaaaatt atcactcagg cattcctgta
     6841 caaattcctg aacgacaaat tcgcttttga ggccaaacag aaagataaaa gtatcgccag
     6901 cgccgaaagc tgggaagatg cgctcagcgc aatgagcgaa gatcaactga aaaagctgca
     6961 acagcggatg gccccggata ccgcccgcct gaagccgcac cattttattc gttacttgta
     7021 taaccgacaa aacgcagcgg actttgccag aacttttgac gacaccctga tggacatcgc
     7081 cgccaccaac aacgacgtct tcgcggtcaa aaccgacggc ggcgcgaaag tggtgctgtt
     7141 tgagcgcctc agccagtaca tcgccgacga atcaaagcgc gatgatttct gccgcgccat
     7201 catcaacaag ctggccgatt tcagcttcga acgcatcttc acgcagaaat tcgacttcta
     7261 cgccaccatc tttgaatacc tgatcaaaga ctacaacagc aactccggcg gcaaatacgc
     7321 ggaatactac acgccccacg ccgtggcgcg catcatggcg gaaatcctgg tgccgaaagc
     7381 gcagcagggc gtggtgcgca acgtaagctg ttacgacccg tccgccggtt ccggcacgct
     7441 gctgatgaac gtggcgcacg ccattgggga agatcgttgc agcatctttg cccaggatat
     7501 ctcgcagaaa tcctccagcc tgctgcgtct gaacctgatc ctcaacaacc tggtgcattc
     7561 gatccctaac gttatccagg gcaacactat tctgcatccg ttccacaagg acggcggcgc
     7621 gctgaaacgc tttgactaca tcgtctccaa cccgccgttt aagatggatt tcagcgactt
     7681 tcgtgacgcg ctggacagca aggaaaacca gcaacgtttc tttgccggga taccgaaaat
     7741 caaagccaaa gccagggaca aaatggagat ttaccagttg ttcctgcaac acatcatttt
     7801 ctcgctgaaa ccgggtggca aggcggcggt cgtcgtgccg accgggttta tcaccgcgca
     7861 atcgggcatt gataaaggca ttcgcgaaca tctggtgcaa aacaagatgc tggcgggcgt
     7921 ggtttccatg ccgtcgaata tcttcgccac caccggtacc aacgtgtcga tcctgtttat
     7981 tgacgccagc aacaaagaga aagtggtgct gatcgacgcc tcgaatttag gtgaaaaggt
     8041 caaagacggc aaaaaccaga aaacggtgct gacggagtgc gaagaaaaac ggatctgcga
     8101 agcgtttaac aacaagtgga gcgaagagga tttctcggtg gtggtcagct atgacgacat
     8161 cgccgcgaaa aattactcgt tcagcgccgg acagtatttt gatgtgaaaa tcgaatacac
     8221 cgacatgacg ccggaacagt ttgccgcgaa gatgaaagga tttacggaga atctgaataa
     8281 tctctttgaa cagtcgcgcg agttagaagt gcaaataaaa atgcagatgg cagatatcaa
     8341 at
//
LOCUS       DAPQZD010000197         8312 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPQZD010000197 DAPQZD010000000
VERSION     DAPQZD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.199"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..738)
                     /locus_tag="RO454_004358"
     CDS             complement(<1..738)
                     /locus_tag="RO454_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDY0936095.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVFSYPAAAITLIGSGALRGAGNTKIPL"
     gene            743..818
                     /locus_tag="RO454_004359"
     tRNA            743..818
                     /locus_tag="RO454_004359"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Asn,seq:gtt)
     gene            complement(856..1806)
                     /gene="cbl"
                     /locus_tag="RO454_004360"
     CDS             complement(856..1806)
                     /gene="cbl"
                     /locus_tag="RO454_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDY0936096.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1908..2825)
                     /gene="nac"
                     /locus_tag="RO454_004361"
     CDS             complement(1908..2825)
                     /gene="nac"
                     /locus_tag="RO454_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDY0936097.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3152..3227
                     /locus_tag="RO454_004362"
     tRNA            3152..3227
                     /locus_tag="RO454_004362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3185..3187,aa:Asn,seq:gtt)
     gene            complement(3283..4218)
                     /gene="ldtA"
                     /locus_tag="RO454_004363"
     CDS             complement(3283..4218)
                     /gene="ldtA"
                     /locus_tag="RO454_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDY0936098.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4280..5359)
                     /gene="cobT"
                     /locus_tag="RO454_004364"
     CDS             complement(4280..5359)
                     /gene="cobT"
                     /locus_tag="RO454_004364"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDY0936099.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5371..6114)
                     /gene="cobS"
                     /locus_tag="RO454_004365"
     CDS             complement(5371..6114)
                     /gene="cobS"
                     /locus_tag="RO454_004365"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDY0936100.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(6111..6656)
                     /gene="cobU"
                     /locus_tag="RO454_004366"
     CDS             complement(6111..6656)
                     /gene="cobU"
                     /locus_tag="RO454_004366"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDY0936101.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2285 a         2168 c         1770 g         2089 t
ORIGIN      
        1 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc
       61 agctgctgga taactgaata cggtcagctc cagataagtc aacgccagtg ctttaacgtc
      121 tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaattagcgt
      181 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
      241 tcgtcgtcga tctcgcttac cgagactaaa tgctacaacg acagtagtac caagatcgat
      301 agctgcaaaa aaagccataa tgaccatatt gaagctgtcc gccaacccca cgccggccat
      361 cgcatctttt cccagccagc tgaccagaaa agtgctcagg acccccatca acaggacaca
      421 ggcgttctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
      481 gtagctcttg cgtttagagt gccagcgagt gccgtgaaca acctggcgta aagcagagga
      541 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaatg atgagggaga
      601 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
      661 tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaatat ttttcgccgt
      721 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
      781 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
      841 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
      901 gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata
      961 gttacgctga agttgccctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg
     1021 ccgcgtatcc aggcggatta aattcttttc ctcttgttcg ccgctggatt gttcggcaac
     1081 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgtgcact
     1141 taatacaata tctgccggca aacctttgcg ggcaaatgcg tcatcaatgc gtgagcgccc
     1201 cgtaatcccc tgtcggtaag taattaacgg ccacttcgct attgattcca gcgtcaacgg
     1261 tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa ctatggtgcc aacgaaacca
     1321 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     1381 ttcgccattt tgcaacaacg tcgcaatttc ctgtggtgta ccctggatta gctccagccg
     1441 aacctccggg aaaagttcgc gaaaagcttt aatgacctca ggcaagctat aacgtgcctg
     1501 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcgag
     1561 ccgacgaaca ttactggctt cattcagaat gcgttctgca atgaccagta atgctttgcc
     1621 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     1681 atcctccagt tcccgaatat gacggctaac gcctgactgt gaggtaaaaa gcatattcgc
     1741 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     1801 attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct aatgcccggc
     1861 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     1921 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     1981 gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa agagagactc
     2041 attgaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgca
     2101 gccgattcag gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     2161 atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa agcctcatca
     2221 acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg cgcaatagca
     2281 ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc
     2341 agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac cgccatatcg
     2401 agttggtgat ttatcagttt ttcgttaagc actgcaccac tattttcatg aagatagata
     2461 acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     2521 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgactgacc aacgttatgc
     2581 accgccagtt gggcctgctc acactgacgt aaaatggccc gtgcatgggt atagagaatt
     2641 tttccggcgc ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg ttgatttaac
     2701 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     2761 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     2821 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagaa
     2881 tgcaagatgc ttgccagttc tattttagcg ttgatgcgca agctaaccaa ctgtcaaata
     2941 agagattatg atagattcgt cattggccct ttttatcagc agtcgcattc acctgcccta
     3001 taaaaggggg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     3061 tgttgaaatt gtcagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     3121 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     3181 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     3241 ctgctttcga gcaggctttt tactttctaa ttaccaacgc tcttaaaaca tctgtcttga
     3301 accagaacta atttgcacag gcattcccga tcgatgttgc aacgcagcat ttgcgcgatt
     3361 tacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     3421 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     3481 ctttgaacca tccggttccg ttgtgtattt cactggctga tcaataattt gtacacgagt
     3541 gcctacagga acattatcaa acagatattt gatatcgtca ttacgcagac gaatacagcc
     3601 ctgacttacg cggagcccaa taccaaaatt ggcattggta ccgtggatgg catacaactt
     3661 gccaatataa atcgcgtaca gccccatggg gttatcgggc cccgcaggaa caaatgcggg
     3721 caaactctcc cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg
     3781 tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     3841 gccgatacca ataggaaaga cttccactgt attactgtct ggcggatagt aataaagacg
     3901 catttcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     3961 cggaatggtg agttgtgagc caggcttcgg caaaaaaaca tcagcgcccg gattcgcttc
     4021 cagcatatta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgagtatt
     4081 gtgatcagga acagttacag taagcgactg ccccactaaa cggctaccct ctggaggtaa
     4141 tggataagtt accgccaggc tattttggct ggcaaaaagc agagcaaatg agcaaagaat
     4201 atttacacga cgcatcatgt ccctttccta agtcgcgaat actatccgat aagtatagct
     4261 tttatcagac ttttcgtttt tagctgttca aatcagaagt cgtattcccc ggtagaacaa
     4321 tattactggc agcaagttcg cccatgttgt tgtatatcgc acaagcagct tcgatgatgg
     4381 acatcgccag agctgcacca ctcccctcac ctaaacgcat atccatattg agataaggct
     4441 ccagccccaa atgcgagagc gctatacgcg cgcctttttc tgccgacaag tgagaaggaa
     4501 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa
     4561 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc acctaacatc actccagcta
     4621 ttccgaccaa atcaaatcca cccacttttg ccaggacatc aacaccatcc tgaggatttg
     4681 gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt attagccagt ttatctgtcg
     4741 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     4801 ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     4861 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgac
     4921 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     4981 taagcccggg gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc
     5041 ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg ggtcatattt tcagcctgta
     5101 tggctgtcac ttcttttgga gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     5161 acataaccag tactgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcctg
     5221 ccagttgtat ggcaagcgcc tccagctttc ccaggctacc aacaggtttg agtaacccgt
     5281 caacatgccg ttgtgcacgc gacatagcgg cagaattgat ggctgggatc gtattcagta
     5341 aatcggcaag tatttgcatg tcacgtctct ttatagcagt gccagcagga aaaccagttc
     5401 accaaactct atggctgctc ccagcgtatc gcccgtttgc ccgcccagcg tacgtttgag
     5461 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     5521 acccggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     5581 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgcgcat aacgatgacg
     5641 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     5701 gatcggttcg ccacgcagag ccaactcgct taacaccaga atctttgcca gtaccacaaa
     5761 aatcaatgcc agaccgccgt gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     5821 atcgcggcta cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa
     5881 tcccccggtc atcagcgcca gcacaagtac gctaaacagt gccgccagtg gtacgccaca
     5941 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa
     6001 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     6061 cggtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt
     6121 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga
     6181 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
     6241 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     6301 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
     6361 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattccagc
     6421 aacacagcct cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc
     6481 tccactgtgc gccagtgctc cgggcggctt tgccgatgat gttctatccg tgcagccatc
     6541 tcatcatcaa ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc
     6601 acctctgcgt ggcgactctt cccgctccgt gcgcctcccg tcaccaaaat catcataccg
     6661 actcctgatt caaatgtatg atggtattca ttgaacagag caatggatcc cccaccgttg
     6721 ctgaccgcaa aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt
     6781 ctcaatccat tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt
     6841 ccgacaaaat gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg
     6901 aattaatgct actgccggac caaccagcaa acgggcattg tgttctgaaa tccaggctat
     6961 tgattcaacg tcagcgacag atctgcgctg taaaagaagt tgtaaaacaa cgcaataaaa
     7021 ttatccggaa tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc
     7081 actgtcacag cattcaacag aatgtgactt gccagattta ttagcgccac cagatgttta
     7141 accgggtaac cacgacccag tcggggatat attccatctt ctcgctgaca tcatcaagaa
     7201 taaaaaggtt tatcacactc agagcaacgt tcttcagcag gcgggataat tcttcaacat
     7261 ttaaaagatg tgttattaaa ggcatacaaa ctggacgtat tgtattttct tttggtggtt
     7321 gacctaaggt agcagtttat cctgatgcgc tgagatttct gtaatatcta cgtcaacatt
     7381 ctcctcgaat agaaaatgca gcctcggcaa gtgattcaca ttttttaaca aagcgttgtt
     7441 agcgtgccat tttcagaagc tctccaagaa agcgaattct tttctcaaat tctgcattct
     7501 cagacacagc tttctactcc atggcaatgg cccaaatacg aagttgctca ggatcattag
     7561 tggtgctgag aagttaaata accatgcgga gtacaactcg tcatcacgta tttatatatc
     7621 atgaagctat tgttgtgttt tgtacatcag tagatgtatt ctgatatact cctttttcta
     7681 gacataacct ttcacctgct tgcaaagctt ctgtgttctg acattgccaa attgttgcaa
     7741 ttctgtatcc agccttcttt cagtcatagc ttcgggccgc gataagactc actgatctga
     7801 ccctgattcc tcttgcagac tttatagacc aattaaaatg cagtttctgc aggtcaacgt
     7861 ctgaccatca ttgtcatcac tctggccatt agagtaacct tctgcattca tccttttgta
     7921 aaaagtttat gtcagtatca gcaattaacc ggacctgata ctgatatgag tcttaccgca
     7981 tatacggtca atttcagcaa ttaattacat tatccacgcc aaagtgtttg tcatcgcaat
     8041 gatggtacct tctttcagac accatttttt caactccgtt ttccacggac cgcactctta
     8101 tgtcaagagt acggtccgtg gatacaacca gagaccgact gacacgagtc agaggaaacg
     8161 acggatatgt tcagtcgtaa aatatctatc aaaaaacatg attaaggtca aaaatgtttg
     8221 atatttacaa tttatgagga tgacaataat cacagataca tgagaacata aatgataata
     8281 attatcatta cagcaatcat ttgtactttg ta
//
LOCUS       DAPQZD010000198         8293 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPQZD010000198 DAPQZD010000000
VERSION     DAPQZD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.200"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..367)
                     /locus_tag="RO454_004367"
     CDS             complement(<1..367)
                     /locus_tag="RO454_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936102.1"
                     /translation="MNKNESISYTEMQKFFAREIKEVGGRPITSKNLELSLLPIHARE
                     NLSAPEMPWYTEVNDIYMEFRRSPFWMVAGYVFLGFMLSLLSISILSIVAFSFYEMGF
                     NSDLLFLYLLAFVLGCFILP"
     gene            657..899
                     /locus_tag="RO454_004368"
     CDS             657..899
                     /locus_tag="RO454_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936103.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYLRKLREKW"
     gene            complement(1081..2010)
                     /gene="fdhE"
                     /locus_tag="RO454_004369"
     CDS             complement(1081..2010)
                     /gene="fdhE"
                     /locus_tag="RO454_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDY0936104.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2007..2642)
                     /gene="fdoI"
                     /locus_tag="RO454_004370"
     CDS             complement(2007..2642)
                     /gene="fdoI"
                     /locus_tag="RO454_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDY0936105.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2639..3541)
                     /gene="fdoH"
                     /locus_tag="RO454_004371"
     CDS             complement(2639..3541)
                     /gene="fdoH"
                     /locus_tag="RO454_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDY0936106.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3554..6604)
                     /gene="fdnG"
                     /locus_tag="RO454_004372"
     CDS             complement(3554..6604)
                     /gene="fdnG"
                     /locus_tag="RO454_004372"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6017..6019),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDY0936107.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6798..7631
                     /gene="fdhD"
                     /locus_tag="RO454_004373"
     CDS             6798..7631
                     /gene="fdhD"
                     /locus_tag="RO454_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDY0936108.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7713..8273
                     /locus_tag="RO454_004374"
                     /pseudo
     CDS             7713..8273
                     /locus_tag="RO454_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1965 a         2117 c         2164 g         2047 t
ORIGIN      
        1 caggtaatat aaaacagcca agcacaaatg caagtagata cagaaataac aagtcactat
       61 taaagcccat ctcataaaat gagaatgcaa caatagataa tatcgagatg gaaagtagtg
      121 aaagcataaa tcccaggaat acataaccgg caaccatcca gaatggtgag cgtctgaact
      181 ccatatagat atcattaact tcggtatacc acggcatttc tggcgcagaa aggttttccc
      241 tggcgtgaat gggtagcaga cttaactcta aattcttaga agtgatgggc ctaccgccta
      301 cttccttaat ttctctggca aaaaattttt gcatttcggt gtaagaaatt gattcgtttt
      361 tattcattat atctgttccg ttccctgacg acttatcgtt ttttcttggt agatgacatt
      421 aagctattgg atgtgagaaa ggcaacttta tagagtaaat tcagaaagag tagaaatttt
      481 cacacgactg ttttcacttc cattttttac tggttttcgt aataactgca actgttacat
      541 cgtatctgga aaacgcctcg caaaaataaa aagtgatgcg taaatgcgct ttctttaaag
      601 attcgacaag ggtacttttc tctccataga ttcaaaatga tattggcgag atatttatga
      661 accctctggc aggtatggat atgagcagaa ttttactcga tttatcgaat gaggtgatta
      721 agcaacttga tgatcttgag gtgcagcgta atcttccgcg tgcagaccta ttacgggaag
      781 cggtagatca atacctggta aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
      841 ggcaagggtg tgaggaagat ggtgtcgaat acctgcgtaa gctgcgcgag aaatggtaat
      901 ggcaaaaaga tttggctatt tgatatcgat atccttatcg attttctcag cgtgaaaatt
      961 gaggccaaac aggcactggc ggcatagctt gaggaggtaa cgcggaatac gagagatagt
     1021 gtcgtcacac cctatgaact gtaatccggg cgacctgcgc cacccggaaa acgtcagaga
     1081 ttactcccct tcacccggaa acaggaacgg gttgatggaa ctacgggcat agccttcttg
     1141 ctccattcgc gcgtccagta ccagagaggc gaggtcatct gccacggctt caactttcgg
     1201 ttctttttcc tgataaagaa tcttcaggta agtgccgcag tcatcgcagc tttcggcttt
     1261 aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct gttcacagtt
     1321 gctgcatttt acgcgcacta cgtgccattc agtttcacac aggttgcagt gcaggtaacg
     1381 cagaccctga gtggtgccaa tttgcaccat gctggacacc ggcatagagc cacataccgg
     1441 gcaatattga cgttgttcgc cgtattcagc gcgggctttg ccggggatca gattagccat
     1501 ctgcgcccag tagagcgaca gcgcagccca gataaacggc gctttatcgc tgctgacgga
     1561 cgagaaatca gaggcaaaca gtgcgctggc catatcttcc agctcctgag tcgatgcctt
     1621 ctccagattc tcaatcactg ccagcgccgg gccgctcatt tcaggtttca gctcagcaat
     1681 cagcgccatc agcagctttt gccagtgctt atcacgcggc agaacgtgaa tatccagcgg
     1741 gggcttgcct tgtgcgctgg cttctttaat gcgcgtggtc agatccatct ccagcggatg
     1801 gtcgtacagc accacttcct gggcgtgggc gataagcgca gcaaagcgca ggtaatcacc
     1861 cagcggatta tgttctgcca gctcgcgcag acgctcggcg cggcggttgt ataaattctt
     1921 gagccgaggg aacaataacg gcggaatcat atccgccgta cgtttctcgc tcgaacccag
     1981 ctcatcttgc gggattatgc gaatactcat tcagctttct tttccgttgt cttgcggacc
     2041 tcacggtacc agcgcgggtg atgtttcttt gcccatgcgc tggtcaccca tccttccacc
     2101 atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat gataaccaca
     2161 attaacgcca ctgcggcaaa tgaatgcagc attaacgcga atcggatcac cgggattgag
     2221 aaagcaggcg caaaataagg acgccagata atcacgccgc tcaccagcaa caggaccagg
     2281 aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt tataacgccc ggtgtcacct
     2341 acttcctcgt tgacgacgat cttacgaata ttcttcgccc aaaagatatc atcccgattg
     2401 attaggttgt gatgccagta acggaaaaac atgatgatga acgaggcaaa cataaccacg
     2461 ccgacaaacg gatgcagaat tcgcgccagc tgcggtgtgc ccatgatttg catcaaccag
     2521 ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa gcagaaggcg
     2581 gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc acgtcgtttc
     2641 atttgcgctc ctcgtctttc tcttcgtgca gattattctc ttcctcatcc gcacggttcg
     2701 gaccgacacc cacgtagtgg aagatactgg ctgcgaaggt agccgcaaag ccaacagctg
     2761 cgagtggttt ccagatgcct ttccagaatt tcacggtttc gctgatttcc gggttctccg
     2821 gcaagccatg atacagattt ggcttgtcag catggtgcag tacgtacata acgtgtgtac
     2881 caccgacgcc tgccggatcg tacagacccg cattgtcgta accgcgggtt ttcagctccg
     2941 ccacgcgctc gctcgccagc gttttcatcg actctttcgt accgaagtga atcgcgcccg
     3001 ttgggcaggt cttcacgcag gccggttctt gcccaaccac cacgcggtca acgcacaacg
     3061 tacatttgta gacgcggttg tcttccgggt tgaggcgtgg cacgttgaac gggcaccctg
     3121 cgatgcaata accgcagccg atgcactgct cggactggaa gtcgacgata ccattggcat
     3181 actgaataat tgccccttcc gccgggcatg ctttcaggca gcctggatcg gaacagtgca
     3241 tacagccgtc tttgcggatc agccattcca gtttgtcgtt ctgctccact tccgagaatc
     3301 gcatcaccgt ccacgatttg gcacttaaat cattggggtt gtcatacacc ccaatgttat
     3361 tgccgacggt atcgcgaatg tcgttccact ctgaacacgc cacctgacag gctttacagc
     3421 cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc ctggaagtcc cgcgcctgag
     3481 gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat atcttgcgat tgataagcca
     3541 tatgtcgtct ccgttacacc ttttccacat tcacaaggaa ggacttaaac tccggcgtct
     3601 gcgtgttcgc atcaccgacg aatggcgtca acgtattggc aataaagcct tttttcgcaa
     3661 caccttcata gccccagtga atcggaatac cgatggtatc gatatctttg ccgtttgctt
     3721 tcagcgtgcg aatacgtttg gtcactaccg ctttggcttt gatatagcca cggttggagg
     3781 agactttcac agtatcgccc tgggcaatgc caagtttatt cgccagcgac tccccgattt
     3841 ccacaaactg ctctggttgc aaaatcgcgt tcaacagcgc gtgtttggtc cagtagtgga
     3901 agtgctcggt cagacgatag gtggttccga catacgggaa cttatcggct ttccccaatg
     3961 cttcggcatc gtctttaaag atacgcgccg ccggattcga gataacgttt ggatgcagcg
     4021 ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga ccttccgcca
     4081 tcttatcgag ggcaaacaga cgccccatgc cttcctgctg catgataaac ggcccgacgc
     4141 cactgcccgg aggcgctgcg ctgtagtccg gaatatccca gccggtccac ttagtgccgt
     4201 cccatttcag caactgacgc ttcggatccc acgggttacc ctgcggatct gcggaggcgc
     4261 ggttatacag aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc gtgttaccga
     4321 ggccagacgg atcggcgtta tcacgacgcg ccatctggtt gccttctggc gtccagctac
     4381 cggcgaaaat ccagcagcca caggaggttg taccgtcatc gcgcagttga gcgaacgagc
     4441 taagttgttg gcctttcttg acaataaccg caccggttgc cggatcggta atatcggcca
     4501 gcgccttacc gttgctctcc atcgccactt cttccgactt cggctcatgc gggatggcgt
     4561 agttccaggt catgttcagc acctggtccg ggttcgcgcc accctgttcg gcatacatct
     4621 tgcgcaagcg caggaagata ccggagagga tctcgccatc agtcagcgca atccccgggg
     4681 cgtccgcacc tttccagtgc cactgcaacc agcgaccaga gttaacgatt gaaccgttct
     4741 cttccgcgaa gcaggtcgat ggcagacgga acacttcggt ctggatcttc gacgagtcaa
     4801 cttcgttcag ttcaccgtgg ttctgccaga agttagaggt ttcagtgttc agcgggtcga
     4861 tagtcacgag gaatttcagc ttcgacagac agccgatcac tttgtttttg ttcgggaatg
     4921 aggcaacagg gttaaagccc tggcagatat agccattgac cttgccctct ttcatcatct
     4981 cgaagtattg caggacgtcg tagcctttat cccacttcgg caaccagtca aagccccagc
     5041 tattttccgc cgtcgcttta tcaccaaaga aggccttcat catagagacg aagaatttcg
     5101 ggtagttgcc ccagtagtta acctggcctt ccagcagtgg tttcggcgtg ttggcggtaa
     5161 ggtaagtttg cagatcggtc tgcttctcgc ttggcagcgt catgtaacct ggcagactct
     5221 gcgacagcag ccccaggtcc gtcagcccct gaatattgga gtgaccgcgc agggcgttaa
     5281 cgccgccgcc agccatcccc atattgccga gcagcaactg gatcatcgcc atcgtacgga
     5341 tgttctgcgc accgatggag tgttgcgtcc agccgagggc gtaaaggaac gaggcagttt
     5401 tatcgtgagc actggtctct gcgatgtatt cgcagacttt caggaatgcg tcttttggcg
     5461 taccgcagat gttttcaacc acatctggcg tgtagcggga aacgtgctgt ttcagcaagt
     5521 tccacacgca acgtggatgt tgcagggtag tatcgcgttt ggcgaagccg ttttcgtcca
     5581 gttcgtaagt ccaggtggac ttatcgtact tgcgtttttc cgcgtcgtag ccggtgaaca
     5641 ggccatcttc aaagccgtaa tcctcacgca cgatcaggct agcgttggta taggcttcgg
     5701 tgtattcgcg gttgaatttt tcattgttca gcaggtacag caatacgcct gacaggaaag
     5761 caatgtcagt accggaacgg ataggggcat agtagtcagc caccgccgct gtacgcgtaa
     5821 agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat tttggcttcc atcgcccagc
     5881 ggaacccgac cgggtgagct tcagcggcgt taccgcccat caccacgacg aggttggcat
     5941 tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg
     6001 ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata cccagcgcgc
     6061 gggagaattt ttgcgttaaa tagccggttt cgttgctcga cgcggaagca cacagcatcc
     6121 cggtggagag ccagcggtta acagtcacgc cttcggcgtt ttgcgcaatg tagtttgcat
     6181 cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa ctgatttgct
     6241 gccatttatc agaacctggc gcacggtatt ccggaaactt cagacggctt tcggagtgga
     6301 tgaaatccac caggccagcg cctttcggac aaagtgctcc gcggttgacc gggtgatccg
     6361 ggtcaccttc gatatggaag atagatgctt tggcgttttt tgctccgtca ccgaggctgt
     6421 acatcaacag cccacagcct acggaacaat aggtgcaggt attacgggtt tcgcgggtgc
     6481 gcagcagttt atactgccgg gtttccgcga gtgctacgct gggtgcaaaa cccagtgccg
     6541 ctgccgtggt gcctgccata ccgccagcgc agatcttaaa gaactgcctt ctgctgacct
     6601 gcatggattg ctccttgttt cgacattgtc acgtcccatt tacattcgct tgctgcgtgt
     6661 gcagggagtg ggagttattt ttctttgcgg aaggggccgc aaaggtccag aattggctca
     6721 atttccctcc atccaggaag ggtttgtaac agaataccat aatgttggtg tgtgtgttct
     6781 tatctggtta atagaaagtg aaaaaaacac agcaaaaaga aatcgagaat gtgacaaata
     6841 tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga tttacaacac ccacggttag
     6901 atgaggtggc ggaagaagtt cccgttgcgc tggtctacaa cggcatttcg cacgtggtga
     6961 tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg tttttcgctt tccgaaggaa
     7021 ttatcgaaag cccgcgcgat atcttcggca tggatgtcgt tccttcctgt aatggcctgg
     7081 aagtacaaat tgagctttcc agccgccgct ttatggggtt gaaggaacgt cgccgggcgc
     7141 tggcgggacg tacgggctgt ggcgtatgcg gtgtagaaca gcttaacgat atcggtaaac
     7201 cggtgcagcc gctgccgttc acccagacgt ttgatctcaa caaactggat gatgcattac
     7261 gtcatctcaa tgatttccag ccagtggggc aactgactgg ttgtactcac gccgctgcat
     7321 ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc catgtggcgc
     7381 tggacaaact gttaggccgc cgatcacaag aaggggaaag ctggcagcaa ggtgcggtgc
     7441 tggtttccag ccgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgc ggcgtagaga
     7501 ttttgtttgc ggtgtctgcc gcgaccacac ttgctgtaga agtggctgag cgctgtaatc
     7561 tgacgctggt gggcttttgt aaaccgggtc gggcgacggt ttatacccat ccgcagcgtt
     7621 tgagcaatta attaaaataa gtcctgcgat atatattaaa ttacaggact tattcatttc
     7681 gtgaattttg ttattttttt ataaacaaaa cgcgtgtagt ggcgttaatg cctgcgctgc
     7741 aacctattga tggcgtggcg gtgagttata ttgatgctgc cgtagcgttg ggtaatacca
     7801 ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg tttgcgaaag
     7861 ggaaaacatt ccactactac tctctggcag tcatgatttc agcgaaacaa attaataacc
     7921 tgatatcgca agataagttt gatgcagaag cggcaatgaa gaaagtgtct gaactggaaa
     7981 cgctggtggc gcaagccaaa gaagcggata aaagcggcat gaatttctcg tttattaatt
     8041 cggcaggcca gtatcaactc gaggctaaaa aatacgttcg ccgcatcaga gataaagtcc
     8101 cgtactctga ctgggataaa gagcaacttc aggacgcaaa ctcaagctgg atggccgaag
     8161 actcatttcc acgagcgtta tgcgattaca acgaaatggt cgatgaaata tttcagttaa
     8221 ttgtaatcgc ggggcgggtt tgtgatgagc acggatatgt cacgaagtcg taacaatata
     8281 aagcagagtg aag
//
LOCUS       DAPQZD010000199         8236 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPQZD010000199 DAPQZD010000000
VERSION     DAPQZD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.201"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            132..962
                     /locus_tag="RO454_004375"
     CDS             132..962
                     /locus_tag="RO454_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDY0936109.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            994..1929
                     /locus_tag="RO454_004376"
     CDS             994..1929
                     /locus_tag="RO454_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDY0936110.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2022..2264
                     /locus_tag="RO454_004377"
     CDS             2022..2264
                     /locus_tag="RO454_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDY0936111.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            2254..3705
                     /gene="panF"
                     /locus_tag="RO454_004378"
     CDS             2254..3705
                     /gene="panF"
                     /locus_tag="RO454_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDY0936112.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3717..4598
                     /gene="prmA"
                     /locus_tag="RO454_004379"
     CDS             3717..4598
                     /gene="prmA"
                     /locus_tag="RO454_004379"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDY0936113.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4927..5892
                     /gene="dusB"
                     /locus_tag="RO454_004380"
     CDS             4927..5892
                     /gene="dusB"
                     /locus_tag="RO454_004380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDY0936114.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5918..6214
                     /gene="fis"
                     /locus_tag="RO454_004381"
     CDS             5918..6214
                     /gene="fis"
                     /locus_tag="RO454_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDY0936115.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6299..7183
                     /gene="yhdJ"
                     /locus_tag="RO454_004382"
     CDS             6299..7183
                     /gene="yhdJ"
                     /locus_tag="RO454_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDY0936116.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7267..7446
                     /locus_tag="RO454_004383"
     CDS             7267..7446
                     /locus_tag="RO454_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDY0936117.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7449..8111)
                     /gene="acrS"
                     /locus_tag="RO454_004384"
     CDS             complement(7449..8111)
                     /gene="acrS"
                     /locus_tag="RO454_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDY0936118.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         2035 a         1974 c         2071 g         2156 t
ORIGIN      
        1 tatttttcaa tgtattaaga tattttagct gcaattttga cccatgattt tcttttcgaa
       61 ttgtcgatat gattgaagga taactttcaa agaaaaatac aaggggctat agtgttacgc
      121 attaaaggcc aatgaagagt gttaacgtgc cgagaaaatc gtctaactta ctgaaacgta
      181 gattaaaaat tgcagaaatt gttgccgccc agggtgaaat caaagttgat gacttatctg
      241 cgcaacttgg tgtctctggc gtgacgatcc gcggcgatct cagttatctg gagcaacagg
      301 gatatttaaa gcgatcattt ggcggtgcta ttgcgacacc actctcctct gaaccagaag
      361 cgatacaacc gatcgcgcca ttgtctctgg cacaacaaat ggaaatagca cgacattgcg
      421 cacggttaat tcgcgatcgt gacacgctat ttcttggaca tgggacaatc tgccgtaaga
      481 tcattccatt gctcagcgaa gtgaaaaaac tgcgtctgat caccaacgat cccgaacatg
      541 cgctgctggc aaatcagttt attgatggcg aaataatact ggctggcagt gaaatgctgc
      601 gccccgacac cgcactggcc ggaaaacagc ttgaacaggc acttcagcat ttcaccatta
      661 cacatagcat tctggaaatc agccatctgg atgctgatgg tactttaaat atcaacgttc
      721 ccagactcgc cgctgcctgg cagctctgtt tcgatcatgc ccagaataag acggttatcg
      781 ttgccgccga tcctgcgcca tcaacggctg ctatcgggca tttgcatcag gcagaaaatg
      841 tgatcgtcag ccgcagcatt aacgaacaat atcagcaaca attgcagtcg gctgatttcg
      901 ttatccttta taccagtaat gagtgcttta cctggtccaa tcgcgaaagg cttcaggaat
      961 gagaggggtt ttgtgacaat aactatctgc gctatgggcg aattgctggc cgagtttttg
     1021 tcccgcaacc cacatcaaaa attcactcag cctggggagt ttatcgggcc atttcccagc
     1081 ggtgcgccag caatttttgc tgctcaggtg gcaaaactgt cccatcgggc catcttcttt
     1141 ggatgtgttg gtaatgatga ttttgcccga ctcattatag agcgtctccg tcatgaaggt
     1201 gtcattaccg atgggatcca tgttatgaac aatgccgtca caggtacggc gttcgtgagt
     1261 tatcaaaatc cccagctgcg ggatttcgtc tttaatatcc ctaacagcgc ctgcggtttg
     1321 tttactgccg agcacattga taaggatctg cttaaacagt gtaaccatct gcatattgtg
     1381 ggctcatcgt tgttctcatt tcgcatgatc gatgtcatgc gtaaagcaat aacgacgatc
     1441 aaatcggctg gcggcaccgt ttctttcgat cccaatattc gcaaagagat gctgagcatt
     1501 cctgaaatgg cgcaggctct cgattatttg attgaatata cggatatttt tatccccagc
     1561 gaaagcgaac tccctttctt cgcgcgtcac aaaaatctgt cagaggaaca gattgttagc
     1621 gatcttctcc acggcggcgt aaaacatgtg gcgataaaac gcgcccagcg tggggctagc
     1681 tattacaagc ttaaaaacgg tacattacac gcccagcatg ttgcaggtca cgatatcgaa
     1741 attatcgatc caacgggtgc aggcgactgc tttggcgcaa cgtttatcac tcttttctta
     1801 tccggtttcc cggcacacaa ggcgctgcaa tatgcaaatg ccagcggcgc gctcgccgta
     1861 atgcggcaag gtccgatgga agggatatcc tcactggcag acattgaaga ctttttgcag
     1921 cagcactgaa cggcaatttt ctgtcagatt attttccctg cctgaagact tgccgtagaa
     1981 ttgcccgttt ctcgtatccc cccatgagga ggaccccttc cgtggacact cgttttgttc
     2041 aggcccataa agaggcgcgc tgggcgctgg ggctgaccct tttgtatctg gcggtatggt
     2101 tagtagccgc ttacttacct ggcgttgccc ccggttttac cggttttccg cgctggtttg
     2161 agatggcctg catcctgacg ccgctgctgt ttattggact gtgctgggcg atggtgagat
     2221 ttatctatcg cgatatccca ctggaggatg acgatgcagc ttgaagtaat tctaccgctg
     2281 gtcgcctatc tggtggtggt gttcggtatc tctgtttatg cgatgcgtaa acggagcact
     2341 ggcaccttcc ttaatgagta tttcctcggc agccgctcta tgggcggtat tgtgctggcg
     2401 atgacgctca ccgcgaccta tatcagtgcc agttcgttta tcggcgggcc tggagctgct
     2461 tataaatacg gcctgggctg ggtattgctg gcgatgattc agcttcctgc agtctggctt
     2521 tcgctcggta ttctcggcaa gaagttcgca attcttgcgc gccgctacaa tgcggtgacg
     2581 ctgaacgata tgctgtttgc ccgctaccag agtcgtcttc tggtgtggct ggcgagtttg
     2641 agtttgctgg ttgcgttcgt tggtgcgatg accgtgcagt ttatcggcgg tgcgcgcctg
     2701 ctggaaaccg cggcgggtat tccttatgaa accgggctgc tgattttcgg tatcagcatt
     2761 gcgttgtata ccgcctttgg cggctttcgc gccagcgtgc tgaacgacac catgcaaggg
     2821 ctggtgatgc tgattggcac cgttgtgctg cttattggcg tggtacatgc cgcaggtggt
     2881 ttaagtaatg cggtagacac gctgcaaacc atcgatccgc aactggttac gccacaaggc
     2941 gctgacgata ttctgtcgcc tgcctttatg acgtcgttct gggtactggt gtgctttggc
     3001 gttataggcc tgccgcatac tgcggtgcgc tgtatctctt ataaagacag caaagcagta
     3061 catcggggga tcatcatcgg tacgattgta gtcgcaatcc tgatgttcgg tatgcatctg
     3121 gccggagcgt taggtcgggc ggtgatcccc gatctcaccg taccggatct ggtgatcccg
     3181 acgttaatgg taaaagtgct gccaccgttt gccgccggga tcttcctggc tgcaccgatg
     3241 gctgcgatca tgtcgacaat taacgcccaa ctgctgcaaa gttccgctac gatcattaaa
     3301 gatctctatc tgaatatccg tccggatcaa atgcaaaacg agacgcgtct gaagcggatg
     3361 tcggcggtga tcacgttggt tctcggtgcg ttgctgctgc ttgccgcctg gaagccgcca
     3421 gaaatgatca tctggctgaa tttgctggcc ttcggtgggc tggaagccgt tttcctgtgg
     3481 ccgctggtgc tgggtcttta ctgggaacgc gccaacgcca aaggcgcgct aagtgcgatg
     3541 atcgttggcg gcgtgctgta tgccgtactc gcgaagctga atattcagta cctgggcttc
     3601 caccctatcg tgccctcgtt actactaagt ttgctggctt tcctggtcgg aaaccgtttc
     3661 ggtacatccg tcccgcaagc taccgttttg actactgata aataaagagt tttgccatgc
     3721 cttggatcca actgaaactg aacaccaccg gcgcaaacgc ggaagatctt agcgatgcgc
     3781 tgatggaagc gggtgccgtt tctatcactt ttcaggatac ccacgatacg ccagtatttg
     3841 agccgctgcc gggcgaaacg cgcctgtggg gcgacaccga tgtgattggt ctgttcgacg
     3901 ctgaaaccga tatgaacgac gtggtggcga ttctggaaaa ccatccgctg ctcggcgcgg
     3961 gcttcgcgca taaaatcgaa caactggaag ataaagactg ggagcgcgaa tggatggata
     4021 atttccaccc gatgcgcttt ggtgaacgac tgtggatctg cccaagctgg cgtgatgtgc
     4081 cggacgaaaa cgccgtcaac gtgatgttag acccagggct ggcgtttggt acgggtaccc
     4141 atccaactac ctctctgtgc ctgcaatggc tcgacagcct cgatttaacc ggtaaaacag
     4201 tcatcgactt tggctgcggt tccggcattc tggctatcgc ggcgctgaaa ctgggtgcag
     4261 caaaagccat tggtattgat atcgatccgc aggcgatcca ggccagccgc gataacgccg
     4321 aacgtaatgg cgtttctgat cgtctggaac tctacttacc gaaagatcag ccagaagaaa
     4381 tgaaagccga cgtggtggtc gctaacatcc ttgcaggccc attacgtgaa ctggcaccgt
     4441 taatcagcgt cctgccagtt tcaggcggtt tgctgggcct ttccggtatt ctggcaagcc
     4501 aggcagagag cgtttgcgaa gcttatgccg acagcttcgc tctggacccg gtcgtggaaa
     4561 aagaagagtg gtgccgcatt accggtcgta agaattaacc ttcgcatcgc cgtatggcga
     4621 cgcagagcga agtgcgagcg atgtcacaaa aggcacgtta atttgccgat tatttacgca
     4681 aatttgcgtg ccaaaatttt cattcataaa gaaaaattga gaacttactc aaatttcttt
     4741 gagtgtaaat tttagtcact attttctaat atgatgattt ttatgagtaa ttatcgcgct
     4801 acgctcattt taaatgcaat tctttgatcc atctcagagg attggtcaaa gtttggcctt
     4861 tcatctcgtg caaaaaatgc gtaatatacg ccgccttgca gtcacagtat ggtcatttct
     4921 taactcatgc gcatcggaca atatcagctc agaaatcgcc tgatcgcagc gcccatggct
     4981 ggcattacag acagaccttt tcggacgttg tgctacgaga tgggagccgg attgacagta
     5041 tccgagatga tgtcttctaa cccacaggtt tgggaaagcg acaaatctcg tttacggatg
     5101 gtgcacattg atgaacccgg tattcgcacc gtgcaaattg ctggtagcga tccgaaagaa
     5161 atggcagatg cagcacgtat taacgtggaa agcggtgccc agattattga tatcaatatg
     5221 ggttgcccgg ctaaaaaagt gaatcgcaag ctcgcaggtt cagccctctt gcagtacccg
     5281 gatgtcgtta aatcgatcct taccgaggtc gtcaatgcag tggacgttcc tgttaccctg
     5341 aaaattcgca ctggctgggc accggaacac cgtaactgcg aagagattgc ccaactggct
     5401 gaagactgtg gcattcaggc tctgaccatt catggccgta cacgcgcctg tttgttcaat
     5461 ggagaagctg agtacgacag tattcgggca gttaagcaga aagtttccat tccggttatc
     5521 gcgaatggtg acattactga cccgcttaaa gccagagctg tgctcgacta tacaggggcg
     5581 gatgccctga tgataggccg cgcagctcag ggaagaccct ggatctttcg ggaaatccag
     5641 cattatctgg acactgggga gttgctgccc ccgctgcctt tggcagaggt taagcgcttg
     5701 ctttgcgcgc acgttcggga actgcatgac ttttatggtc cggcaaaagg gtaccgaatt
     5761 gcacgtaaac acgtttcctg gtatctccag gaacacgctc caaatgacca gtttcggcgc
     5821 acattcaacg ccattgagga tgccagcgaa cagctggagg cgttggaggc atacttcgaa
     5881 aattttgcgt aaacagaaat aaagagctga cagaactatg ttcgaacaac gcgtaaattc
     5941 tgacgtactg accgtttcta ccgttaactc tcaggatcag gtaacccaaa aacccctgcg
     6001 tgactcggtt aaacaggcac tgaagaacta ttttgctcaa ctgaatggtc aggatgtgaa
     6061 tgacctctat gagctggtac tggctgaagt agaacagccc ctgttggaca tggtgatgca
     6121 atacacccgt ggtaaccaga cccgtgctgc tctgatgatg ggcatcaacc gtggtacgct
     6181 gcgtaaaaaa ttgaaaaaat acggcatgaa ctaattcagg ttagctaatt gcttgattaa
     6241 aaggcgctac tcggcatggg gaagcgcctt ttttataggt gtcacaaagg gagtgaccat
     6301 gagaacagga tgtgaaccga cccgatttgg taatgaagca aagaccatca tccagggtga
     6361 tgcattaacc gaacttaaaa agctccccgc cgaaagtgtc gatctgatct ttgccgaccc
     6421 accgtataac atcggtaaaa attttgatgg tctgatcgaa gcctggaaag aagatctgtt
     6481 tatcgactgg ctgtttgaag tgattgcaga gtgccatcgc gttctgaaaa agcagggcag
     6541 catgtacatc atgaatagca cggaaaacat gccctttatc gatctccagt gccgcaagct
     6601 ttttaccatc aaaagtcgca tcgtctggtc atatgacagt tctggagtgc aggcgaaaaa
     6661 acactacggc tccatgtacg aacccatcct gatgatggtg aaagacgcaa agaactacac
     6721 gttcaacggt gatgctattc tggtagaagc caaaaccgga tcgcagcgcg cgttgatcaa
     6781 ttatcgcaaa aatcctccac agccttacaa tcatcaaaaa gtaccgggta acgtctggga
     6841 ttttccgcgc gtgcgttatt tgatggatga atatgaaaac cacccgacgc aaaaaccgga
     6901 agccttgctg aaacgcatta ttctcgcctc ttccaaccca ggcgatatcg ttctcgaccc
     6961 gtttgccggt agctttacta ccggtgccgt agccgtcgcc agcgggcgaa aattcattgg
     7021 tattgagatc aacagcgagt acatcaaaat ggggcttcga cggctggatg tcgcgtcgca
     7081 ttactctgcg gaagaactgg cgaaagtgaa aaaaagaaag accggcaacc tgtcaaaacg
     7141 aagccggtta agcgaagttg accccgatct cattgcaaag taaaggaagt gtaagcctgg
     7201 ttttcagatt attcatttcg tgtatatttc ctgccagact tggttaaaca tgcacaggct
     7261 caggtaatga ttcgcaagta ttggtggctc gtcgttttcg ctgtcttcgt ttttctgttt
     7321 gatactttac tgatgcagtg gattgaactg ctggcaacag aaacagacaa atgccgcaat
     7381 atgaactcag ttaatccact aaaactggtt aactgtgacg aactgaattt tcaggacaga
     7441 atgtgaattt atatgacact taattcattc gttttatgaa ttaatttcgt tatgttttca
     7501 tctggcatga acattcttaa tacgttatcg accagagcgg gggcttgttt ataaagatca
     7561 taacccgcca tattcattaa ccagttttga acaattccgc tgaaggcacc atcaataata
     7621 atcatcacaa catctaaatc gagattattt gctacacaac cttgttgctg acacgcctgc
     7681 aatacttcgc ggagagtctg cggattaaag cccatctttt cgcgtatcac tccctcggcc
     7741 agcatctcat cattaaattc acatttgtga tataagattt tcagcaacgc ctgctggcgg
     7801 ggaattttgg caatatattg caaaccgaca atcaattttt cacgcaattg ctgaaacggg
     7861 tcatgctcta acccagcggt caagtgatcc tggattaact cccgcaatga aggctgttgc
     7921 aaccacatct cattaaacag ttgagtcttg ttttcgaagt gccagtagat agcgccacgc
     7981 gtaacgttag cagcgtcggc aatgtcgttg agcgtcgtct tgcttacgcc atgctgcgca
     8041 aactgggcga tggcagtttc aatcagttct tgccgggtct tcagggcttc agctttggtt
     8101 ctttttgcca tgattaatta ttcaggaaat aaatatattc gacacaggat gagaaaatag
     8161 cgaaggttaa tctatcacct aatgtgtatt tatacgagag gctaatattg cgttgctata
     8221 aatcgtcaaa taaatg
//
LOCUS       DAPQZD010000200         8108 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPQZD010000200 DAPQZD010000000
VERSION     DAPQZD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.202"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..2852
                     /gene="evgS"
                     /locus_tag="RO454_004385"
     CDS             <1..2852
                     /gene="evgS"
                     /locus_tag="RO454_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDY0936119.1"
                     /translation="SIVLNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELTE
                     HEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNIITLQTGLNFSPITVSH
                     NIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVFVMQKAPDSEQTLKKGM
                     KVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHY
                     YPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNV
                     TIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALS
                     DSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSESPFKDVFSNTHEVTA
                     ETKENRKIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHAL
                     EVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYA
                     TGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFAAIAASKSIALSCSS
                     TLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGHIDDNHAVIKMTIMDS
                     GSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTIF
                     TITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEA
                     TDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANERE
                     KGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQE
                     ILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITP
                     VSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(2908..4053)
                     /gene="yfdE"
                     /locus_tag="RO454_004386"
     CDS             complement(2908..4053)
                     /gene="yfdE"
                     /locus_tag="RO454_004386"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDY0936120.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4127..5071)
                     /gene="yfdV"
                     /locus_tag="RO454_004387"
     CDS             complement(4127..5071)
                     /gene="yfdV"
                     /locus_tag="RO454_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDY0936121.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5141..6835)
                     /gene="oxc"
                     /locus_tag="RO454_004388"
     CDS             complement(5141..6835)
                     /gene="oxc"
                     /locus_tag="RO454_004388"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDY0936122.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6889..>8108)
                     /gene="frc"
                     /locus_tag="RO454_004389"
     CDS             complement(6889..>8108)
                     /gene="frc"
                     /locus_tag="RO454_004389"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDY0936123.1"
                     /translation="DFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQLRDIPDI
                     DALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFTWEHIQ
                     EINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAAALGDSN
                     TGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYLEEYPQ
                     YPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTCKAIGKP
                     EWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLSMKEIS
                     LDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQELGYS
                     DDEIAAMKQNHAI"
BASE COUNT         2365 a         1898 c         1696 g         2149 t
ORIGIN      
        1 attcaatcgt tcttaatgaa gtactcaatc gatttgttga tgctttaaca aatgaagttc
       61 gctatgaagt atcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat
      121 tagaactcac tgaacatgaa aaacagtgga ttaagcagca tcccgattta aaggtgctgg
      181 aaaatcctta ctcgccaccc tattctatga cagatgaaac tggctcagtt cggggcgtga
      241 tgggtgatat tcttaatatt attaccttgc aaacaggttt aaatttttct ccgatcaccg
      301 tttcacacaa tattcatgct gggacacagc ttaaccccgg tggatgggat atattgcccg
      361 ccgctattta tagtgaagat cgagaaaata atgtttcatt tgctgaagtc ttcataacaa
      421 cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa
      481 tgaaagttgc cattccatat tattatgagc ttcattcgca attaaaagag atgtatccgg
      541 aggtagagtg gataaaagtc gataacgcca gcgctgcatt tcacaaggtc aaggaaggtg
      601 aacttgatgc tctggtcgcg acacagttaa attcgcgtta catgatcgac cattactatc
      661 ctaatgaact ttatcatttt cttattcccg gcgttcagaa tgcatcactt tcgttcgctt
      721 ttcctcgcgg agaaccggaa cttaaggata ttattaataa agcactgaat gcaattcccc
      781 caagcgaagt tctgcgcctg accgaaaaat ggattaaaat gcccaatgtg accattgaca
      841 catgggacct ctatagcgag caattttata ttgttacgac attatccgtt ttattagttg
      901 gcagtagcct tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagttattc
      961 agggtgattt agaaaaccaa atatcattcc ggaaagcgct ctcggattcc ttacctaatc
     1021 caacttatgt tgtaaactgg caaggtaatg tcattagtca caatagtgca tttgaacatt
     1081 atttcactgc tgattactat aaaaatgcaa tgttaccatt agaaaacagt gaatccccct
     1141 ttaaagatgt tttttctaat acgcatgaag tcacagcaga aacgaaagaa aaccgaaaaa
     1201 tatacacaca ggtatttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc
     1261 ataccttatg taatctgcca gcaagcgaac atgctgttta tatttgtggt tggcaagata
     1321 ttaccgagac gcgtgattta attcatgcac tcgaagtaga gagaaataaa gcgatcaatg
     1381 caactgtcgc aaaaagccag tttctggcaa caatgagtca cgaaataaga acaccaataa
     1441 gctccattat gggcttcctg gaacttctgt cgggttctgg tcttagcaag gaacaacggg
     1501 tggaggcgat ttcacttgcc tacgccaccg gacaatcact cctcggctta attggtgaaa
     1561 tccttgatgt cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata
     1621 tccctacttt agtccagaac acttgtcact ctttcgctgc gattgctgca agcaaatcga
     1681 tcgcattaag ttgcagcagt acgcttcctg aacgctacct ggttaagatc gaccctcagg
     1741 cgtttaagca ggtcttatct aatttactga gtaatgctct caaatttact accgaggggg
     1801 cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa ccacgctgtt atcaaaatga
     1861 cgattatgga ttctggaagt ggattatcgc aggaagaaca acaacaactg tttaaacgct
     1921 atagccaaac aagtgcaggt cgtcagcaaa caggttctgg tttaggctta atgatctgca
     1981 aagaattaat taaaaacatg cagggcgatt tgtcattaga aagtcatcca ggcataggaa
     2041 cgatatttac gatcacaatc ccggtagaaa ttacccaaca agtggcggct gtcgaggcaa
     2101 aagcagaaca acctataaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc
     2161 cgaccaacag gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag
     2221 ccactgatgg tgtgcaagcg ctacacaaaa tcagtatgca acattatgat ctgcttatta
     2281 ctgacgttaa tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa
     2341 attcttcctt acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag
     2401 ggttaaattg cggcatgaac ttatgtttgt tcaaaccgtt gactctggat gtactgaaaa
     2461 cacatttaag tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata
     2521 tcgaggccct gaagaataat acggcgaatg atctacaact gatgcaggag attctcatga
     2581 ctttccagca tgaaacgcat aaagatctac ccgctgcgtt tcatgcacta gaagctggcg
     2641 ataacagaac tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt
     2701 tgcaaaagtt gattaatatt agccaccagt tagaaataac acctgtttca gatgacagta
     2761 agcctgaaat tcttcagttg ctgaactctg taaaagaaca cattgcagag ctggaccagg
     2821 agattactgt tttctgtcag caaaatgact aaatagcggc tcccacaatg ttcaaatgtg
     2881 ggagctattt accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc
     2941 atgctggtcg agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt
     3001 tatcggatta cccggcatca ttattccccc ggcttcaatc aacatatttc tcgcctgagt
     3061 ttgtggtaaa ttaatggcct cagccacact taataacggc gcgacgggca caccaacttc
     3121 atgtattctg gctaaccaaa cttcagctac ctgcgttttt aacgtccgct caatatattc
     3181 tttaagaata gcctggttat gtacgcgtaa aatattgctg ctaaatcggg gatcattaac
     3241 cagttccgta agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca
     3301 aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg
     3361 atttcccagg cgttgtggtg acttcccagt cgcgatatat gccatcagtc catgctccag
     3421 aaaactcaga gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc
     3481 gcggccataa agtgcactca ctattccact gaataaataa acaccgccac agagatccgc
     3541 aagagaggta ccaacgcgca ctggcggagc atcagggtat cctgtttcca tcattatccc
     3601 gctcattgcc tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc
     3661 gaaacctgac gatgaagcat atataaggcg cgggttgatt tcttgtaacc tttcccatga
     3721 aaacccgagt ttttccattg tacctgggcg aaaattctca gctaatacat cagcttgttt
     3781 aagcatattt ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc
     3841 tttgccatga ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt
     3901 gcgggtatca tcaccatgac ccggtggctc aactttaatt acccttgcgc ccatattaca
     3961 aagaagttga gttccgaaag gcccattaag gacatgcgtc atatcgataa ctaataagcc
     4021 ttcaaacggc cctttgcatt cattatttgt catttgctta tgccttgaaa aaagccgaag
     4081 gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gataaattat gaaaccagtc
     4141 gactgacata aatccacaaa ggagccgtga cgacaaaacc cagtacgctc acagccaatg
     4201 acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg
     4261 ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg ttcgctattc aaatgacatg
     4321 ccataccgac gagaagcagc gccagtggca tcagaatcag cttcaggaaa gtgttataag
     4381 caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg
     4441 ctacccctga gttagccttc gcaatcagat taaaggttgg gtcccatgct gccggaattt
     4501 ttaccccaac cagcaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg
     4561 ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat
     4621 tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta
     4681 ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga
     4741 atccaatggt aggtgaacct gcaattaatg cacatactgc tgcttctgca tgggtacgtt
     4801 taaaaaattt gtagcaaccg aaccaggaga aaaagaaaca tccgacaata accacaagtg
     4861 atacaagggt caggcgagtg tccgcaaaaa tcatttccct gtttgccctg gtaatagaaa
     4921 caaatagagc cgcaggaaga gcgtagttta ataccagttt attaaatgcc cgggcttgat
     4981 cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag
     5041 gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac
     5101 catcgcataa taagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt
     5161 ttgggttaag tttggtaata tggccacttt cagttcctgc tgctgggtcg atgaccacat
     5221 taataatggt cggtttgcgc gactgaattc cggtggttaa agcatgacga agttcatctg
     5281 tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat
     5341 ggtgcaacag atccgttggc gatggtgcac cagcgccgct gaggtcaaca ccgtctcctc
     5401 tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg
     5461 tttcaatctc catcccactg aaaccaaaag cactatcacc ttcaatagcg acaaccggag
     5521 aaccagaggt cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg
     5581 tgccacaatc cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca
     5641 gggtatttgc cccttcatta actaaataaa tatcctggtt ctcgcgcaaa acatcgcgca
     5701 cggcactcaa tgcattaaag taatttaatg gttgggtatc tgtacttaac ttttcatgca
     5761 ttttttgtgc attttgctgc ttgtggatat ttaaaatatc acgccacacc agtggagtcg
     5821 taaatgtgtt ttgtttcaat tctgccagca taccttgcat actggatgca atatcgccaa
     5881 cgactggcac agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa
     5941 taaactgtgt atctgccgcc catccttttt taccgtgtgc cagtaaccaa ttcagtcgtg
     6001 caccaacaag catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag
     6061 agagtggatg cgtatcttcg aggatccctt tcgccataga cattggcagg aatggaatct
     6121 gggcactttc aataaattca cgaagctgtt catcagattg tgaatacgcc gcgcctttgc
     6181 caagaataat taatggtcgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact
     6241 tcgggcatgg caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt
     6301 cgtctttttc catcgtcgcg gccaggacat ttgctggcaa atcaagataa acaccgccag
     6361 gacgacccga tactgagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct
     6421 gattaacgcg aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt
     6481 cataatcacc ttgctggaga tcgacgatcg cgcggtcgct ggagccgcta atcatgatca
     6541 tcggaaaacc atttaccgtt gcattggcca atgcggtcaa accgttgagg aatcctggcg
     6601 cagacactgt caggcagatc cctggttttt gggtaagaaa accgcttgct gcagcggcat
     6661 aacctgccga ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc
     6721 gcgccatatc cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt
     6781 ttaatgcttc aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta
     6841 ataacctcga aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg
     6901 gttttgcttc attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc
     6961 ggtatgttca cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat
     7021 tggacaacca actgtcagat attttccacg caacggttgt tccacttcga caacactgcc
     7081 actttggcgc aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcgcaagg
     7141 aatatcaaac tgagtcaaat aggccacagc ttcatgttta tcaatagtga cagtgtattt
     7201 ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc
     7261 cggatcggta atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc
     7321 ctgaatagtg aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat
     7381 ccagccaggc tgaccaccac cacccgcatt accaccgcgg ggaactgcat caccaaatgt
     7441 gccattcgga tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc
     7501 gcgtaatttc acgcggcaaa ggttcaacac ggcatcctgc attgacatgg tcactcgttg
     7561 cccacgcccc gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat
     7621 tccggtgttg ctgtcaccca acgctgcagc gcttaccagc ggcggaccgt cccaaaaacc
     7681 tgtagtagat gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac
     7741 ataaggcgaa cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc
     7801 ttgaatatgc tcccaggtga agcccatatg atcaatggcc cctggatgaa agttctcgac
     7861 taagatatca gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt
     7921 atttaattca atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc
     7981 aggaatatct cgcagctggt ggcgcgttac gtcaccaacg ccgggacgtt caattttaat
     8041 gacgtcagcg ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt
     8101 gaaatcga
//
LOCUS       DAPQZD010000201         8017 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPQZD010000201 DAPQZD010000000
VERSION     DAPQZD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.203"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            150..314
                     /locus_tag="RO454_004390"
     CDS             150..314
                     /locus_tag="RO454_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDY0936124.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
     gene            317..484
                     /locus_tag="RO454_004391"
     CDS             317..484
                     /locus_tag="RO454_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936125.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            598..693
                     /locus_tag="RO454_004392"
     CDS             598..693
                     /locus_tag="RO454_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936126.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            843..3518
                     /gene="acnA"
                     /locus_tag="RO454_004393"
     CDS             843..3518
                     /gene="acnA"
                     /locus_tag="RO454_004393"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDY0936127.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSFKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDETIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3582..4172)
                     /gene="ribA"
                     /locus_tag="RO454_004394"
     CDS             complement(3582..4172)
                     /gene="ribA"
                     /locus_tag="RO454_004394"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDY0936128.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4342..5106
                     /gene="pgpB"
                     /locus_tag="RO454_004395"
     CDS             4342..5106
                     /gene="pgpB"
                     /locus_tag="RO454_004395"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDY0936129.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5255..5563
                     /gene="lapA"
                     /locus_tag="RO454_004396"
     CDS             5255..5563
                     /gene="lapA"
                     /locus_tag="RO454_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDY0936130.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            5570..6739
                     /gene="lapB"
                     /locus_tag="RO454_004397"
     CDS             5570..6739
                     /gene="lapB"
                     /locus_tag="RO454_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDY0936131.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQLLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6931..7668
                     /gene="pyrF"
                     /locus_tag="RO454_004398"
     CDS             6931..7668
                     /gene="pyrF"
                     /locus_tag="RO454_004398"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDY0936132.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRDFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7668..7994
                     /gene="yciH"
                     /locus_tag="RO454_004399"
     CDS             7668..7994
                     /gene="yciH"
                     /locus_tag="RO454_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDY0936133.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
BASE COUNT         1966 a         1875 c         2139 g         2037 t
ORIGIN      
        1 cctcgcaaac tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg
       61 agacgattcg taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt
      121 atgattaatt acaccaatcg ttcaacgtct tgctgataag tgatggcgat tatatgaggt
      181 tagcaatgcc ttctggaaat caggaacccc gccgcaatcc tgaattaaaa cgtaaagcct
      241 ggctggcggt ttttcttggt tctgcacttt tctgggtggt tgtcgcactg cttatttgga
      301 aattgtgggg ataaccatgg tcggtcagga gcaactggag tcgtcaccat tatgtcagca
      361 tagtgacaat gaaccagaag caaaacggga atgttccgtc gttattccag acgactggca
      421 actaacatcg cagcaacaag cctttataga actgtttgct gaagatgatc agccgaaaca
      481 ataattatca tcattcttat tacccatttt taatgaatta cagggctttt aatacaccgc
      541 agcaataaca ggttgagtta tctcaacaca aagtaataac cgttaagggt gtagcctatg
      601 atcaacacaa atatgaaata ttggtcctgg atgggcgcat tttctctgtc gatgctcttc
      661 tgggccgaac tcctctggat cattactcac tgatccctga tcccgctgtt tgctttgcct
      721 caaggtttct cctcttttat caatttgggt tgttatcaaa tcgttacgcg atgtttgtgt
      781 tatctttaat attcaccctg aagagaatca gggcttcgca accctgtcat taaggaggag
      841 ctatgtcgtc aaccctacga gaagccagta aggacacgtt gcaggccaaa gataaaactt
      901 accactacta cagcttgccg cttgctgcca aatcactggg cgatatcacc cgtctaccca
      961 agtcattcaa agttttgctc gaaaacctgc tgcgttggca ggatggtaaa tcggttaccg
     1021 aagaggatat ccacgcgctg gcgggatggc tgaaaaatgc ccatgctgac cgtgaaattg
     1081 cctaccgccc ggcaagagtg ctgatgcagg actttaccgg cgtacctgcc gttgttgatc
     1141 tggcggcaat gcgcgaagcg gttaaacgcc tcggcggcga tactgcaaag gttaatccgc
     1201 tctcaccggt cgacctggtc attgaccact cggtgaccgt cgatcgtttt ggtgatgatg
     1261 aggcatttga agaaaacgta cgcctggaaa tggagcgcaa ccacgaacgt tatgtgttcc
     1321 tgaaatgggg aaagcaggcg ttcagtcggt ttagcgtcgt gccgccaggc acaggcattt
     1381 gccatcaggt taacctcgaa tatctcggca aagcagtgtg gagcgaattg caggacggtg
     1441 aatggattgc ttatccggat acactcgttg gtactgactc gcacaccacc atgatcaacg
     1501 gccttggcgt gctggggtgg ggcgttggtg gcatcgaagc agaagccgca atgttaggcc
     1561 agccggtttc catgcttatc ccggacgtag tgggctttaa acttaccgga aaattacgtg
     1621 aaggtattac cgccacagac ctggttctca ctgttaccca aatgttgcgc aaacatggcg
     1681 tggtggggaa attcgtcgaa ttttatggtg atgggctgga ttcactaccg ctggcggatc
     1741 gcgccaccat tgccaatatg tcgccagaat atggtgccac ctgtggcttc ttcccaatcg
     1801 atgctgtcac cctcgattac atgcgtttaa gcgggcgcag tgaagatcag gtcgagttgg
     1861 tcgaaaaata tgccaaagcg cagggcatgt ggcgtaatcc gggcgatgaa acaattttta
     1921 ccagtgtgtt agaactggat atgaatgacg ttgaagcgag cctggcaggg ccaaaacgcc
     1981 cgcaggatcg cgttgcactg cccgatgtac caaaagcatt tgctgccagt agcgaactgg
     2041 aagtgaatgc cacgcataaa gatcgcctgc cggtcgatta tgttatgaac ggacatcagt
     2101 atcagttacc tgatggcgct gtggtcattg ctgcgataac gtcgtgcacc aacacctcta
     2161 acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa agccgtaact ctgggcctca
     2221 agcggcaacc atgggtcaaa gcgtcgctgg caccgggttc gaaagtcgtt tctgattatc
     2281 tggcaaaagc gaaactgaca ccgtatctcg atgaactggg atttaacctt gtgggatacg
     2341 gttgtaccac ctgtattggt aactcgggac cgttgcccga tcctatcgaa acggcaatca
     2401 aaaaaggcga tttaaccgtc ggtgcagtgc tgtccggcaa ccgtaacttt gaaggccgta
     2461 tccatccgct ggttaaaacc aactggctgg cctcgccgcc gctggtggtt gcctatgcgc
     2521 tggcgggaaa tatgaatatc aatctggctt ctgagcctat cggccatgat cgcaaaggcg
     2581 agccggtatt tctgaaagat atctggccat cggcacaaga aattgcccgt gcggtagacc
     2641 aagtctccac agaaatgttc cgcaaagagt acgcagaagt ttttgaaggc acagccgagt
     2701 ggaaggaaat taacgtcaca cgatccgata cctacggttg gcaggaggat tcaacctata
     2761 ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc agcacctgtg gaagatattc
     2821 acggtgcgcg gatcctcgca atgctggggg attcagtcac cactgaccat atctctccgg
     2881 cgggcagtat taagcccgac agcccggcgg gtcgatatct acaaggtcgg ggtgttgagc
     2941 ggaaagactt taactcctat ggttcgcggc gtggtaacca tgaagtgatg atgcgcggca
     3001 ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg gggatgacgc
     3061 gccatttacc tgacagcgat gtcgtctcta tttatgatgc cgcaatgcgc tataagcagg
     3121 agcaaacgcc gctggcggtg attgccggga aagagtatgg atcaggctcc agccgtgact
     3181 gggcggcaaa aggtccgcgt ctgcttggta ttcgtgttgt gattgccgaa tcgtttgaac
     3241 gaattcaccg ttcgaattta ataggcatgg gcatcctgcc gctggaattt ccacaaggtg
     3301 tgacgcgtaa aacgttagag cttaccgggg aagagaagat tgatattggt gatctgcaaa
     3361 atctacaacc cggcgcgacg gttccggtga cgcttacgcg cgcggatggt agccaggaag
     3421 tcgtaccctg ccattgtcgt atcgacaccg cgacggagtt gacctactac cagaacgacg
     3481 gcattttgca ttatgtcatt cgtaatatgt tgaagtaacg agtatttgct tgccggttat
     3541 tttgcttccg gcaagcaaat taattacaca atgcaagagg gttatttgtt cagcaaatga
     3601 cccattttct cggctttggt atcgagataa tgttcgttat tggggttgcg acctacaatc
     3661 aacggtacgc gttcaataat attaatccct gcttcggtca gaatttcgac ttttttcggg
     3721 ttattggtta acaagcggac ttcattgacg ccaaggagtt tgaacatatc agcgcaaaga
     3781 gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt tagcctctac agtatcgtaa
     3841 ccttgatcct gcagtgcgta agcgcggatt ttattcagca gaccaatgtt acgaccttcc
     3901 tgacggtgat acagcaaaat accacggcct tcctcggcaa tttgcgtcaa tgccgcttcg
     3961 agctggaagc cacaatcgca gcgcaagctg aacagggcgt caccggtcag acattcggaa
     4021 tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc catagactag cgcgacatga
     4081 tcgtgtccgg ttgccagttc ttcaaatccc accatcagga aatcgcccca tggggttggc
     4141 agtttggctt ctgccacacg tttaagctgc atgaaattct ccagataatg ctggttctgt
     4201 attggcttat tttgccataa cgagaagggg ttcacctaat cacgacgcgt cgatcgttca
     4261 cggaatggca caaatctgtc aatttttcct ggaactggca ttttcagtta tgattgtggg
     4321 acttatcaaa aaggagaggc catgcgttcg attgccagac gtaccgcagt gggagctgca
     4381 ctattgcttg tcatgccagt agccgtatgg atttctggct ggcgttggca acctggagaa
     4441 caaagttggt tactaaaagc ggctttttgg gttactgaaa ctgtcaccca gccctggggc
     4501 gtcattacac atttgatttt attcggctgg tttctctggt gtctgcgttt tcgcattaag
     4561 gctgccatta tgttatttgc cattctggcg gcagcaatcc ttatgggaca aggcgttaaa
     4621 tcctggatca aagacaaagt ccaggaacca cgaccttttg ttatctggct ggaaaaaaca
     4681 catcatattc cggttgatga gttctacact ttaaagcgag cagaacgcgg aaatctggtg
     4741 aaagaacagt tggctgaaga gaaaaatatc ccacaatatt tgcgttcaca ctggcagaaa
     4801 gagacggggt ttgcctttcc ttccggtcac acgatgtttg ctgccagttg ggcactgctg
     4861 gccgttggtt tgctgtggcc gcgtcggcga acgttaacca ttgctatctt gttggtctgg
     4921 gcaacgggag tcatgggaag ccgcctgctg ctcgggatgc attggccacg cgatctggtt
     4981 gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa cgtggcttgc gcaacgaatt
     5041 tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa tagcgcaacg agaacaagaa
     5101 agttaacgct ggttgatttt ccgaatttag cccttaaatc atcaacaatg cgcgtggatg
     5161 ccatttcgca gacggcgcga aaatggtact ttaaagggct attgcggtaa gttgaccata
     5221 atttattcgc tctaaccaca taacgggaag taatgtgaaa tatttactca ttttcttact
     5281 ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg cagaacgatc aacaggtgac
     5341 gtttaattat ctgttagcgc aaggggagta ccgtatttcc acattgctgg cggtattgtt
     5401 tgctgcgggg tttgctatcg gttggttgat ttgcggcctg ttctggctgc gagttcgtgt
     5461 ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt cacccgcgac
     5521 tgacgtggct gtagcgccgc actcgtctgc ggcgaaggaa taactttcta tgctggagtt
     5581 gttgtttctg cttttgcctg tagccgctgc ctatggctgg tatatgggcc gcagaagtgc
     5641 gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt gattacgtag cgggggttaa
     5701 cttcctgctt agtaatcaac aggataaagc ggtagacctg tttctcgata tgcttaaaga
     5761 ggatacaggt accgttgaag cccaccttac gctcggaaac ctgttccgtt cgcgtggcga
     5821 agttgatcgc gctattcgca tccatcagac cctaatggaa agcgcctcgc tgacctatga
     5881 acagcggctt ttggcgattc aacaactggg gcgtgattac atggctgccg ggttatacga
     5941 ccgcgcggaa gacatgttca atcagctgac agatgaaact gacttccgca tcggcgcgct
     6001 gcaacaactg ctacaaattt accaggctac cagcgagtgg cagaaagcaa ttgatgttgc
     6061 cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc atttctactg
     6121 tgagttagct ctgcagcata tggccagcga cgatctcgat cgtgccatga ctttgctaaa
     6181 aaaaggtgcg gcggcagata aaaacagcgc ccgcgtatcc atcatgatgg gacgcgtgtt
     6241 tatggcgaaa gcagaatacg ccaaagccgt cgaaagtttg caacgtgtca tatcccagga
     6301 cagagaactg gtcagcgaaa cgctggaaat gctgcaaaca tgctatcagc tgttgggtaa
     6361 aactgccgaa tgggcagaat tcctgcagcg cgcggtggaa gagaacaccg gtgccgatgc
     6421 tgaattgatg cttgcggata tcatcgaagc gcgcgacggt agtgaggctg cacaggtcta
     6481 tattacgcgc cagcttcagc gtcatccgac catgcgtgtg ttccataaat taatggatta
     6541 ccacttaaat gaagcagaag aagggcgtgc caaagagagt ctgatggtgc tgcgtgacat
     6601 ggttggcgag aaggtacgta gtaagcctcg ttatcgctgc cagaaatgtg gttttaccgc
     6661 atacaccctc tactggcatt gtccgtcttg tcgggcctgg tcaaccatta aaccaattcg
     6721 cggtcttgat ggcctgtaat ttttaaaaaa atccgacttt agttacaaca tactaattat
     6781 tgatcttcta ttgtgttccg gtaccgacgg cgcggaaaag cctgccagag gagaaatcgc
     6841 aactgttaat tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact
     6901 tccggtgccc atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc
     6961 gctgttacga attctcctgt agttgttgcc cttgattatc ataatcgcga tgccgcgctg
     7021 gcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt
     7081 acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtgattttga tatctttctt
     7141 gacctgaaat tccacgatat tcccaacact gcagcgcacg ctgtcgctgc tgcggctgac
     7201 ttaggcgtgt ggatggtgaa tgttcatgcc tccggtgggg cgcgtatgat ggccgcagcg
     7261 cgtgaggcac tggttccgtt tggcaaagat gcaccgcttt tgattgctgt gacagtgttg
     7321 accagcatgg aagcctgcga cctggccgat cttggcgtga cactgtcacc tgcagattat
     7381 gcagaacgtc tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct
     7441 caggaagctg tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacgccgggc
     7501 attcgtccgc aggggagtga tgctggcgac cagcgccgca ttatgacgcc agaacaggcg
     7561 ttggcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca
     7621 gcgcagacgc tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca
     7681 acagccgtct ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggcccctg
     7741 tacgccctaa aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga cgcaaaggta
     7801 agggcgtttg cctgattacc ggtgtcgatc tcgatgatgc cgaactgacg aaactggcag
     7861 ctgaactgaa gaaaaaatgc ggctgcggcg gagcagtaaa agatggaatt attgaaatcc
     7921 agggcgataa gcgtgattta ttaaaatcaa tgcttgaagc gaaagggatg aaggtaaaac
     7981 ttgcaggcgg ctaacataaa aagccacgga tatatcc
//
LOCUS       DAPQZD010000202         8004 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPQZD010000202 DAPQZD010000000
VERSION     DAPQZD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.204"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            98..1081
                     /locus_tag="RO454_004400"
     CDS             98..1081
                     /locus_tag="RO454_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDY0936134.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1153..2301
                     /locus_tag="RO454_004401"
     CDS             1153..2301
                     /locus_tag="RO454_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HDY0936135.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2325..4001
                     /locus_tag="RO454_004402"
     CDS             2325..4001
                     /locus_tag="RO454_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HDY0936136.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4011..4751
                     /gene="kdsB"
                     /locus_tag="RO454_004403"
     CDS             4011..4751
                     /gene="kdsB"
                     /locus_tag="RO454_004403"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDY0936137.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAAEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4748..6775
                     /locus_tag="RO454_004404"
     CDS             4748..6775
                     /locus_tag="RO454_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDY0936138.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFAALRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERTPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTIADLIYQALIVYPTYI
                     HPTLLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6810..7979
                     /locus_tag="RO454_004405"
     CDS             6810..7979
                     /locus_tag="RO454_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDY0936139.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSSYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERILYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYY"
BASE COUNT         2055 a         1944 c         2074 g         1931 t
ORIGIN      
        1 aaaaattttg ttacaaccca ttgatttagc ataaataaat tatagtgggt tcgggtttgt
       61 tgtgactgtg gcattatttc cgtgcaaagg agctgatatg tctgaaagac atttacctga
      121 tgaccagagc agtactatcg atccatatct aattacctct gttcgccaga ctctggcaga
      181 acaaagcgca gcattacaaa acttgtctaa acaactggat tccgggcagt accagcgtgt
      241 ccttaatttg ataatgaact gtaaagggca cgttattctt tcgggaatgg gtaaatcagg
      301 gcatgtcggt cgtaaaatgt cagcgacgct ggcctctacg ggtacgccta gtttctttat
      361 tcatcctgca gaagctttcc atggcgatct gggcatgatt acgccttacg atcttctgat
      421 ccttatttct gccagcggtg aaacggatga aatcctcaag ctagttcctt cactgaaaaa
      481 tttcggcaac cgaattatcg ccattaccaa taatggaaat tccacgctgg cgaaaaatgc
      541 tgatgccgtg ctggaactcc acatggcgaa tgaaacctgc ccgaataatc ttgcaccaac
      601 aacgtctacc acgctgacga tggcgatcgg cgatgcgctg gcgattgcca tgatccacca
      661 acgcaaattt atgccgaatg attttgcgcg ctatcacccg ggcggttcat taggtcgtcg
      721 cctgctgacc cgcgttgctg atgtcatgca gcatgatgtt cctgcggtac agctggatgc
      781 gtcatttaaa accgtgattc aacgtatcac cagcggatgc cagggaatgg tgatggtaga
      841 agacgcagaa ggtgggctag cgggcattat caccgacggt gacctgcgtc gctttatgga
      901 aaaagaggat tctctgacat ccgccacggc tgcgcagatg atgacacgtg aaccgctgac
      961 gctaccggaa gacaccatga tcattgaagc ggaagaaaaa atgcaaaagc accgcgtctc
     1021 aacgttattg gtgaccaaca aggcaaataa agtcactggc cttgtgcgca ttttcgacta
     1081 attaagcaac ggggtgcgag aggttagtct ctcattgagt ttgcgcccca ctgaaacttt
     1141 gataatcgtt acatgttgat aaaagtgaag tctgccgtat cctggatgcg tgctcgtctg
     1201 tctgccatct cactggcaga tatccaaaaa cacctggcga aaatcatcat tctggcaccg
     1261 atggcggtgc tgctgatcta tctggctatc ttcagccagc ctcgctatat gagcgagtcg
     1321 aaagtcgcca ttaaacgctc ggatgattta aacagcggca gcctgaattt tggtctgctt
     1381 ctgggtgcct ctaaccccag ttccgcagaa gatgcgttgt atctgaaaga gtacatcaac
     1441 tcgccggata tgctggcggc gctggataag caactaaatt ttcgtgaagc gtttagccac
     1501 agcgggctcg attttcttaa tcatcttagc aaggatgaaa ccgcagaagg cttcctgaag
     1561 tactacaagg accgtatcaa cgtctcgtat gacgataaaa ccggattact gaatattcag
     1621 acgcagggct ttagcccgga gtttgcgctt aagtttaacc aaaccgtgct gaaagagtca
     1681 gagcgcttta tcaatgagat gtcacatcgc atcgcgcgtg accagcttgc ctttgcagaa
     1741 acggagatgg aaaaggcacg ccagcgtctg gacgccagca aagcggaatt gctctcttat
     1801 caggacaaca acaacgttct ggatccacag gcacaggcac aggcggcgag cacgttagtg
     1861 aatacgctga tgggccagaa gatccagatg gaagcggacc tgcggaactt gctgacgtat
     1921 ctgcgtgagg acgccccgca agttgtgagt gcgcgtaatg cgattcagtc attgcaggca
     1981 caaattgacg aagaaaaaag caaaatcact gcgccacagg gtgacaagct aaaccgtatg
     2041 gcagtggatt ttgaagaaat caaatcaaaa gtagagttca acaccgagct gtacaaactg
     2101 accctgacct ccattgaaaa gacccgtgta gaagcggctc gtaagctcaa ggtgctgtca
     2161 gtgatcagtt cgccacagtt gccgcaggaa tcgtcttttc caaatatccc ttatttgatc
     2221 gcctgctggt tactggtgtg ctgcctgctg ttcggcaccc tgaaactgtt gctggctgtt
     2281 attgaagatc accgagacta acgctgtcgc tgaatgagtt tgtgatgaaa ttatttaaat
     2341 caattttact gattgccgcc tgtcacgcgg cgcaggccag cgcgaccatt gatattaacg
     2401 ctgacccaaa cctgacagga gccgcgccgc ttaccggtat tctgaacgga cagaagtcgg
     2461 atacgcaaaa catgagcggc ttcgacaata ccccgccgcc cgcaccgccg gtggtaatga
     2521 gccgtatgtt tggtgctcaa cttttcaacg gcaccagcgc ggatagcggt gcgacggtag
     2581 gattcaaccc tgactatatt ctgaatccgg gtgatagcat tcaggttcgc ttgtggggtg
     2641 cgttcacctt tgatggtgcg ttacaggttg atcccaaagg taatattttc ctgccgaacg
     2701 ttggtccggt gaaagttgct ggcgtcagta atagtcagct aaatgccttg gtcacatcca
     2761 aagtgaagga agtataccag tccaacgtca acgtctacgc ctccttatta caggcgcagc
     2821 cagtaaaagt gtacgtgacc ggatttgtgc gtaatcctgg tctgtatggc ggtgtgacgt
     2881 ctgattcgtt actcaattat ctgatcaagg ctggcggcgt tgatccagag cgcggaagtt
     2941 acgttgatat tgtggtcaag cgcggtaacc gcgtgcgctc caacgtcaac ctgtacgact
     3001 tcctgctgaa cggcaaactg ggactttcgc agttcgccga tggtgacacc atcatcgtcg
     3061 ggccacgtca gcatactttc agcgttcagg gcgatgtctt taacagctac gactttgagt
     3121 tccgcgaaag cagcattccc gtaacggaag cgttgagctg ggcgcgccct aagcctggcg
     3181 cgactcacat tacgattatg cgtaaacagg ggctgcaaaa acgcagcgaa tactatccga
     3241 tcagttctgc gccaggccgt atgttgcaaa atggcgatac cttaatcgtg agcactgacc
     3301 gctatgccgg caccattcag gtgcgggttg aaggcgcaca ctccggtgaa catgccatgg
     3361 tattgcctta tggttccact atgcgtgcgg ttctggaaaa agtccgcccg aacagcatgt
     3421 cgcagatgaa cgcagttcag ctttatcgcc catcagtggc tcagcgtcag aaagagatgc
     3481 tgaatctctc gctgcaaaag ctggaggaag cctcactttc tgcccagtca tccaccaaag
     3541 aagaagccag cctgcgaatg caggaagcac aactgatcag ccgctttgtg gcgaaagcac
     3601 gcaccgtggt tccgaaaggt gaagtgatcc tcaacgaatc caatattgat tctgttctgc
     3661 ttgaagatgg cgacgtcatc aatattccgg agaaaacatc gctggttatg gttcatggcg
     3721 aggtgctgtt cccgaacgcg gtgagctggc agaaagggat gaccaccgag gattacatcg
     3781 agaaatgcgg tggcctgacg caaaaatcgg gtaacgccag aattatcgtc attcgtcaga
     3841 acggtgcggc agtcaacgct gaagatgtag attcactcaa gcctggcgat gagattatgg
     3901 ttctgccgaa atatgaatcg aaaaacattg aagttacccg tggtatttcc accatcctct
     3961 atcagctggc ggtgggtgca aaagtgattc tgtctttgta aggggttgaa atgagcaaag
     4021 cagttattgt cattccggct cgctatggct cctcgcgcct gccgggtaag ccactgctcg
     4081 atattgttgg taaaccgatg atccagcatg tttacgagcg tgcgttacag gtggcgggcg
     4141 ttgcggaagt ttgggtggca acagacgatc cgcgtgttga acaggccgta caggcgtttg
     4201 gcgggaaagc catcatgacg cgcaacgatc atgaatccgg caccgatcgg ctggtcgagg
     4261 tgatgcataa agtcgaggca gatatctaca ttaacctgca gggcgacgaa ccaatgattc
     4321 gcccgcggga tgtagaaacg ctgctacaag gaatgcgtga cgatcccgcg ttgccggtgg
     4381 caacgctatg ccacgcgatt tctgccgcag aagcggctga gccaagcacg gtaaaagtgg
     4441 ttgtgaatac ccgccaggat gcgctttatt tcagccgctc accgattccg tatccgcgta
     4501 atgctgaaaa agcgcgctac ctgaaacacg tcggtattta cgcttatcgt cgcgatgtgc
     4561 tgcaaaacta cagccagtta ccggagtcca tgccggagca ggcggaatca ctggagcagc
     4621 taaggttgat gaacgcgggg atcaacatcc gcacatttga ggttgccgca accggtccgg
     4681 gcgtcgacac cccagcatgc ctggaaaaag tgcgcgccct gatggcacag gaactggctg
     4741 aaaacgcatg attggcattt actcgcctgg catctggcgt attccgcatc tggagaaatt
     4801 tctggcgcaa ccgtgccaga aactttctct gctgcgccct gttccacaag aagttgatgc
     4861 tatcgccgtg tggggacatc gtcccagcgc ggcgaaacca gtcgccatcg ccaaagcagc
     4921 gggaaaaccc gtcattcgtc tggaagatgg atttgtgcgt tcgctggatc ttggcgtcaa
     4981 tggcgagccg ccgctttctc tggtggtgga tgattgttgc atttactacg atgccagcaa
     5041 gccttcagca ctggagaaac tggtacaaga taaagccgga aatacagctc tgataagcca
     5101 ggccagagaa gcgatgcaca ccatcgtgac cggggatttg tcgaaatata acctggcacc
     5161 tgcgtttgtg gctgatgagt cagaacgttc agacatcgtt ctggttgtcg atcagacatt
     5221 taatgatatg tcagtgacgt atggcaatgc tggcccgcat gagtttgctg ccatgctgga
     5281 agccgcgatg gcggaaaatc ctcaagccga aatttgggtg aaggtgcatc cggatgtcct
     5341 ggaaggaaag aaaacaggtt atttcgccgc tctgcgcgcc acgcaacgag tacgtttaat
     5401 tgccgagaat gtcagcccgc agtcgctgtt gcgacacgtt tcccgggttt acgtcgtgac
     5461 ctcccagtac ggctttgaag ccttgctggc aggaaaaccg gtgacatgct tcggccagcc
     5521 ctggtatgca ggctggggct taaccgacga tcgccatccg cagtccgctt tgttatctgc
     5581 ccgacgcggt tctgccacgc tggaggaact ttttgccgct gcatacctgc gttactgtcg
     5641 ctatatcgat ccgcaaacgg gagaagtaag caatctattt accgtgctgc aatggctgca
     5701 attacaacgt cgacatctgc aacagcgtaa tggttattta tgggcgccag gcttaacgct
     5761 gtggaagtca gcgatcctga aacctttctt gcaaacggca acaaaccggc tgagtttttc
     5821 acgtcgctgt actgcggcga gcgcctgcgt ggtatggggt gtaaagggag aacagcaatg
     5881 gcgagccgaa gcgcagcgaa aatcactgcc gttatggcga atggaagatg gttttctacg
     5941 ttcatccgga cttggctctg accttttgcc gccgctatcg ttggtcctgg ataaacgcgg
     6001 gatctactat gacgccacgc gccccagcga cctggaagtg ctgcttaatc acagccagtt
     6061 aacactggcg cagcagatgc gagctgaaaa attacgccag cgactggttg aaagcaaact
     6121 gagcaaatac aacctggggg ccgatttctc tctgcctgcc gaggccaaag ataaaaaaat
     6181 catcctggtg ccgggtcagg tagaagacga tgcctcaatt aaaacaggca ctgtgtcgat
     6241 taagagcaac cttgagttat tacgcacagt acgcgagcgc actccgcacg cttacattat
     6301 ttataaaccg cacccggatg tactggtggg gaatcgcaag ggcaatattc cgacagaact
     6361 aattgctgaa ctcgctgatt atcaggcact ggacgcagat attattcaat gcattcagcg
     6421 cgcagatgaa gtgcacacca tgacatcatt gtccgggttt gaagcgttat tacatggcaa
     6481 gcaagttcat tgttacggcc tgcccttcta tgccggttgg ggtttaaccg ttgatgaaca
     6541 tcactgcccg cgtcgcgagc aaaaattaac gatagcagat ttgatctatc aggcgttgat
     6601 tgtttatcca acctatatcc acccaacact gctacaacct attacggttg aagaggcggc
     6661 ggaatatttg atccagacgc cgcgcaagcc gatgtttatt acccgaaaaa aagcgggacg
     6721 agtaatacgc tattaccgca aattaattat gttctgcaag gtcagatttg gctaaacaat
     6781 ttcacagttg actattacac aaattattta tgcaaggtaa tgcactaacc gttttattat
     6841 ccggtaaaaa atatctgcta ttgcaggggc caatgggacc ctttttcagt gatgttgccg
     6901 agtggctaga gtcattaggt cgtaacgctg tgaatgttgt attcaacggt ggggatcgtt
     6961 tttactgccg ccatcgacaa tacctagctt actaccagac accgaaagag tttcccggat
     7021 ggttacggga tctccaccgg caatatgact ttgacacaat cctctgcttt ggcgactgcc
     7081 gcccattgca taaagaagca aaacgctggg caaagtcgaa agggatccgc ttcctggcat
     7141 ttgaagaagg atatttacgc ccgcaattta ttaccgttga agaaggcgga gtgaacgcat
     7201 attcatcgct accgcgcgat ccggattttt atcgtaagtt accagatatg cctacgccgc
     7261 acgttgagaa cttaaaacct tcaacgatga aacgtatagg ccatgctatg tggtattacc
     7321 tgatgggctg gcattaccgt catgagtttc ctcgctaccg ccaccacaaa tcattttccc
     7381 cctggtatga agcacgttgc tgggttcgtg catactggcg caagcaactt tacaaggtaa
     7441 cacagcgtaa ggtattaccg aggttaatga acgaactgga ccagcgttat tatcttgctg
     7501 ttttgcaggt gtataacgat agccagattc gtaaccacag cagttataac gatgtgcgtg
     7561 actatattaa tgaagtcatg tactcatttt cgcgtaaagc gccgaaagaa agttatttgg
     7621 tgatcaaaca tcatccgatg gatcgtggtc acagactcta tcgaccatta attaaacggt
     7681 tgagtaagga atatggctta ggtgagcgaa tcctttatgt gcacgatctc ccgatgccgg
     7741 aattattacg ccatgcaaaa gcggtggtga cgattaacag tacggcgggg atctctgcgc
     7801 tgattcataa caaaccactc aaagtgatgg gcaatgccct gtacgacatc aagggcttga
     7861 cgtatcaagg gcatttgcac cagttctggc aggctgattt taaaccagat atgaaactgt
     7921 ttaagaagtt tcgtgggtat ttattggtga agacgcaggt taatgcggtt tattattaac
     7981 atcttatcaa caatcgacat ctcc
//
LOCUS       DAPQZD010000203         7706 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPQZD010000203 DAPQZD010000000
VERSION     DAPQZD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.205"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            4..1200
                     /locus_tag="RO454_004406"
                     /pseudo
     CDS             4..1200
                     /locus_tag="RO454_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    267..383
                     /locus_tag="RO454_004406"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1222..1455
                     /locus_tag="RO454_004407"
                     /pseudo
     CDS             1222..1455
                     /locus_tag="RO454_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1532..1840
                     /locus_tag="RO454_004408"
                     /pseudo
     CDS             1532..1840
                     /locus_tag="RO454_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1899..3209)
                     /locus_tag="RO454_004409"
     CDS             complement(1899..3209)
                     /locus_tag="RO454_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDY0936140.1"
                     /translation="MFKPLTALTVGLSLALSGAALAKEKIDFMFPAPVDGKLTMEMTR
                     VIKQFNDSQQDVEVRGIFTGNYDTTKIKAESAQKAGQPPALVIMSANFTTDLALKDEI
                     LPMDELFKYGDQKAGNFLQTEFWPAMHKNAQVMGTTYAIPFHNSTPLLYYNKTMFDRA
                     GIQQPPQTWAELLADAKKLTDESTGQWGIMLPSTNDDFGGWIFSALVRANGGKYFNED
                     YPGEVYYNSPTTIGALRFWQDLIYKDKVMPSGVLNSKQISAAFFSGKLGMAMLSTGAL
                     GFMRENSKDFELGVAMLPAKEQRAVPIGGASLVSFKGISEAQKKAAYRFLTYLVSPEV
                     NGAWSRFTGYFSPRKASYDTPEMKAYLQQDPRAAIALEQLKYAHPWYSTWETVAVRKA
                     MENQLAAVVNDAKVRPEAAVQAAQKEADALMKPYVDKTALSEVK"
     gene            complement(3202..4275)
                     /locus_tag="RO454_004410"
     CDS             complement(3202..4275)
                     /locus_tag="RO454_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0936141.1"
                     /translation="MLSLQNISKRFDGKPALSALSLDIHEGEFVVLVGPSGCGKSTLL
                     RLLAGLEPVSEGQIWLHDENITATTPRDRNFAMIFQNYALFPHLSVRDNITFGMKVRK
                     EEKSSWQPRVDKVAQMLQLEALLDCKPAKLSGGQRQRVAMARAIVRNPRLFLMDEPLC
                     NLDARLRSEVRDSIMALHQQLKTSTIYVTHDQTEAMSMADRIVVMNGGHVQQVGHPEY
                     LYANPANLFVAGFIGSPAMNLLSLPCANGEVLLGEQRHPLPPRHRDQTRVWLGVRPEH
                     ITDHVEEDHLRLPATVLQRELMGADYLLHVSTPIGTLRFSRRNRGKVPEKDESLPIGF
                     SPADVHLFHAETQHNLQMETDHV"
     gene            complement(4281..5105)
                     /locus_tag="RO454_004411"
     CDS             complement(4281..5105)
                     /locus_tag="RO454_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDY0936142.1"
                     /translation="MLLAHISDTHFRSRGEKLYGFIDVNAANADVVSQLNALRERPDA
                     VVVSGDIVNCGRPEEYQVARQILGSLNYPLYLIPGNHDDKAHFLEHLHPLCPQLGNDP
                     QNMRYVVDDFATRLLFIDSSHAGTSKGWLTDETIGWLEAQLFEGGDKPATIFMHHPPL
                     PLGNAQMDPIACENGHRLLELVERFPSLTRIFCGHNHSLTITQYLQALISTIPGTVHQ
                     VPYCHEDTRSYYDLSPASCLMHRQVGEQWVSYQHSLAHHAGPWLYDENISCPTEER"
     gene            complement(5116..6003)
                     /locus_tag="RO454_004412"
     CDS             complement(5116..6003)
                     /locus_tag="RO454_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDY0936143.1"
                     /translation="MSADISPLLVHTPAAARPLWLRLRRSSPFTLTVLMCCLALLWVS
                     PFIWMLTTSFSATTFGDDMASLLPRLPLTLDNFRDAWNSADWLSLYANTIIFTFGTFF
                     VQLLTITTAGYVFACHEFRGKQTLFLLFLVQLMIMPVVMMVPNMLTLKTFGLLNTLIG
                     VMMPYFTSAFGVFLMRKAFLAIPKELEEAALMEGCSWWQVLFRVLLPMSWPSVLAFAT
                     VSITYHWNEYLWPLMMLNDPDKQVLTVGLVSFAMGAESGGQWGTIGAGTLMVCLPLML
                     AFILFQKQFLRSFGFSGIK"
     gene            complement(5993..6871)
                     /locus_tag="RO454_004413"
     CDS             complement(5993..6871)
                     /locus_tag="RO454_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDY0936144.1"
                     /translation="MCVTMRKTWLPWLILSPSLLFLLLFTWFPLGRSVYDSLFDTRMA
                     NAQYVGLDNFARLFADNVFWQSLGNNLLYILLTVMPGVTLALLLAVALSENHRVNRWL
                     RTAFFFPMIIPMVSAAALWLFIFMPGLGLLDHYLAKLFGPMNNNWLGRSNSALLALAL
                     IGVWKFAGYYMLFFLAGLQSIPASTREAALMEGATRTQVFFKVTLPLLRPTLSFVITT
                     ALIYSITQIDHVAVMTRGGPDNATTVLLYYIQNLTWDTHDLGKASAATFLTLAGLFAF
                     SLINLKLLEKGAHYER"
     gene            complement(7002..7442)
                     /locus_tag="RO454_004414"
                     /pseudo
     CDS             complement(7002..7442)
                     /locus_tag="RO454_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224837.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            7416..7610
                     /locus_tag="RO454_004415"
     CDS             7416..7610
                     /locus_tag="RO454_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936145.1"
                     /translation="MAEAADADHPLPVGTQLIGEAAAARLAEKNVLLTPSGRASALAF
                     MMTLRADRRYKDAQPLPLVT"
BASE COUNT         1671 a         2099 c         2363 g         1573 t
ORIGIN      
        1 gggatgataa gtttatcacc accgactatt tgcaacagtg ccctggaaag tcagggcgaa
       61 tatgactcac aatgggcggc aatttgttcc attgctccaa agactggctg tacgccggag
      121 actctgcgtg tctgggttcg ccagtatgag cgggataccg ggggcggaga tggagggctc
      181 accagtgctg aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc
      241 agcaacaata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
      301 tggaaaaagt gatggcactg ctggataagc tgcgtgagca gtacggggtc agaccggtat
      361 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag cgacatcatc
      421 ctgataaacg cagtgcacat gcacagcgcg atgactggct gaagagagag atacagcgcg
      481 tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg
      541 aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg ggacttgccg
      601 gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt gccgcaggtg
      661 accgcgtaaa ccgtcagttc gtggcagaac gtcctgacca gctgtgggtg gctgatttta
      721 cttacgtcag cacatggcag ggcttcgtct atagtggcgt tcatcattga tgtatttgcc
      781 ggatatatcg tgggatggtg ggtatcatcg tccatggaaa cgacattcgt gctggatgca
      841 ctggagcagg cgttgtgggc ccgtcgtccg tccggcacca tccatcacag cgataaaggc
      901 tctcagtatg tgtcactggc ctatacggag cgactaaaag aagcaaaact gctggcatca
      961 acagggagta caggcgactc gtatgacaac gcgatggcgg agagcattaa tggtctttac
     1021 aaagcggagg taatacaccg taagagctgg aaaaaacgtg cagaagtgga actggccaca
     1081 ctcacgtggg tggactggta taacaatcga cgattgctgg gaaggctgag gcatactcct
     1141 ccggcagaag cagaaaaagc gtattatgct tccatcggaa atgatgagct ggcaacctga
     1201 gttcacagat aaaacactct ccaggaaaac cggggcggtt cactggcgct caatattatt
     1261 gttctgtgga acactattta catggatgca gcaattcagc atctcaggcg tgaggagtac
     1321 ccggtcctgg attcagacgt tgagaaactg tcaccattac agtgcgggca tattaatatg
     1381 caagggcgct attcatttat agtgccggaa tcagtcagta agggtgagct gagggcgttc
     1441 aatgaagatg tatgatttat tgattaatag ataattggct attttaatta acctcttaac
     1501 ggcgttactg ttccattgct ccccaaaccc ctttatagcg gtacgggtgc tgggcctgcg
     1561 gcagggaggt atcagtgaag aaacacaaaa tgacagctgt gagaaaatac tggcgccgac
     1621 agagtggaaa ttgctgtggg ttaagctgga aggaaaaccg cttccgtcac agacgccgac
     1681 actgaaatgg gcctgtctga aactggcgaa actggggcga tggtatgaca gcaaacgcac
     1741 aggccgcccc ggttgggtag ttatgtggga tggctggttc agacttcagg atatggttga
     1801 gggttacctg gtgatgaagt ctcttgatca ggagatctga tcaagagaca gccctcaggg
     1861 agagggccag gatgagggtg acgatgacgg gtaacgcgtc acttcacttc cgacaacgcg
     1921 gtcttatcca catagggttt cattagcgcg tcagcttcct tctgcgccgc ctgtaccgcg
     1981 gcttccggcc ttactttggc atcgttgacc accgccgcca gctggttctc catcgcctta
     2041 cgcacggcga cggtctccca ggtggagtac catggatggg cgtacttcag ttgctcaagc
     2101 gcaatcgccg cccgcggatc ctgctgcaga taggccttca tctctggcgt atcgtatgac
     2161 gccttgcgcg gcgagaagta gccggtgaag cggctccatg cgccgttcac ctcggggctc
     2221 accagatagg tcaggaaccg atacgctgct ttcttctgcg cctcgctgat gcctttaaaa
     2281 cttacgaggc tggcgccgcc gatcggcact gcgcgctgct ctttggctgg cagcatagct
     2341 accccaagct caaagtcttt gctgttctca cgcataaagc ccagcgcgcc ggtgctgagc
     2401 atcgccatcc cgagcttgcc ggagaagaac gcggcgctga tctgtttcga gttcagcacc
     2461 ccggacggca tcaccttgtc tttgtagatc agatcctgcc agaagcgcag ggcgccgatt
     2521 gtggtcggcg agttgtaata gacctcgccc ggatagtctt cgttaaagta ttttccgccg
     2581 ttggcgcgca ccagcgccga gaagatccag ccgccgaagt cgtcgttggt cgacggcagc
     2641 atgatccccc actggcctgt gctctcgtcg gtcagctttt tggcatcggc cagcaactcg
     2701 gcccaggtct gcggcggctg ttggatcccg gcccgatcga acatcgtctt gttgtagtag
     2761 aggagcggcg tcgagttatg gaacgggatc gcataggtgg tgcccatcac ctgggcgttc
     2821 ttatgcatcg cgggccagaa ttccgtttgc agaaaattgc cggccttttg atcgccatat
     2881 ttaaacagct catccatcgg caggatctca tccttcagcg ccagatcggt ggtgaagttg
     2941 gcggacatga tcaccagcgc cggtggctgg ccggccttct gcgccgattc ggctttgatc
     3001 ttggtggtgt cgtagttgcc ggtgaagatt ccgcgcactt cgacatcctg ctgcgagtcg
     3061 ttgaattgct taatgacgcg ggtcatctcc atggtcagct tgccgtccac cggcgccggg
     3121 aacataaagt ctattttctc tttcgccagc gccgcgccgc tcagggcaag gctgagccca
     3181 accgtcaggg cggtgagggg tttaaacatg atctgtctcc atctgtaaat tatgctgggt
     3241 ctcagcatga aaaagatgca catcggcagg cgagaagccg atcggtagcg actcgtcttt
     3301 ctccggcacc ttgccgcggt tgcggcggct aaagcgcagg gtgccgatcg gggtgctgac
     3361 gtggagcaga taatcggctc ccatcagttc tcgttgcaga acggtggccg gcaggcgcag
     3421 atggtcctcc tccacgtggt cggtaatatg ttccgggcga acgcccagcc agacgcgggt
     3481 ctgatcccgg tggcgcggcg gcagcggatg gcgctgttct cccaacagta cttcaccgtt
     3541 ggcgcagggc agcgacagga ggttcatcgc cggcgaaccg ataaacccag cgacgaacag
     3601 gttggccggg ttggcgtaca gatactccgg gtgcccgacc tgctgcacgt ggccgccgtt
     3661 catcaccacg atgcggtcgg ccatcgacat cgcctcggtc tgatcgtggg tcacatagat
     3721 ggtgctggtt ttcagctgct gatggagggc cataatgctg tcgcgcactt cgctgcgcag
     3781 gcgggcgtcg aggttgcaga gcggctcgtc catcaggaac agccgcggat tacgcacaat
     3841 cgcccgggcc atcgccaccc gctggcgctg gccgccggag agcttcgccg gtttgcagtc
     3901 gagcagcgcc tccagctgca gcatctgcgc cactttatcc actcgcggct gccagctgct
     3961 tttctcttcc ttgcgcacct tcatgccgaa ggtgatgttg tcgcgcaccg acagatgcgg
     4021 aaacagggca tagttctgga agatcatcgc gaagttgcga tcgcgcggcg tggtggcggt
     4081 gatgttctca tcatgcagcc agatttggcc ttcgctgacc ggttccagcc cggcgagcag
     4141 gcgtagcagg gtgcttttcc cgcagcccga cgggccgacc agcaccacga attcgccttc
     4201 gtggatatcc agcgacaggg cgctgagcgc cggtttgccg tcgaaacgtt tactgatgtt
     4261 ttgcagactg agcatggcag ttagcgctct tccgttgggc aactgatgtt ttcgtcgtac
     4321 agccacggtc cggcgtggtg ggccagcgag tgctggtagc tcacccactg ctcgccgacc
     4381 tgacggtgca tcaggcacga ggccggggag agatcgtaat aggaccgggt atcttcgtgg
     4441 cagtacggca cctgatggac ggtgccagga atggtggaga tcagcgcctg gagatactgg
     4501 gttatggtca ggctgtggtt atggccgcag aagatgcgcg tcagcgacgg aaaacgctcc
     4561 accaactcca gcagacggtg gccattttcg caggcaatag gatccatctg cgcattgccc
     4621 agcggcagcg gcgggtggtg cataaagatc gttgccggtt tgtcgccgcc ctcgaacagc
     4681 tgggcttcca gccagccgat ggtctcgtcg gtcagccagc cttttgacgt gccggcatga
     4741 ctggagtcga taaacagcag gcgggtggcg aagtcatcca ccacatagcg catattttgc
     4801 ggatcgttgc ccagctgcgg gcacagcggg tgaagatgtt cgagaaaatg ggctttgtcg
     4861 tcgtggttgc cggggatcag atacagcgga tagttcaggc tgccgaggat ctggcgggcc
     4921 acctgatact cctccgggcg gccgcagttg acgatatccc cgctcaccac caccgcgtcc
     4981 gggcgctcgc gcagtgcgtt aagctgggag accacgtcgg cgttggcggc attgacgtcg
     5041 ataaagccat acagcttctc gccgcggctg cgaaaatggg tatcggaaat gtgtgctaac
     5101 agcatgaatc actccttatt tgatcccgga gaagccgaag ctccgcagga actgtttctg
     5161 aaaaaggata aacgccagca tcagcggcag gcagaccatc agcgtcccgg cgccgatggt
     5221 gccccactgg ccaccggatt cggcgcccat ggcgaaagag accagcccga cggtcagcac
     5281 ctgcttatcg ggatcgttga gcatcatcag cggccacagg tactcgttcc agtggtaggt
     5341 aatgctgacg gtagcgaagg ccagcaccga cggccaggac atcggcagca gcacgcggaa
     5401 cagcacctgc caccagctgc agccctccat cagcgccgcc tcctccagct ctttcggaat
     5461 ggcgaggaac gccttacgca ttaggaatac gccgaacgcc gaggtgaagt agggcatcat
     5521 caccccgatg agggtgttga gcagaccgaa ggttttcagg gtcagcatgt tcggcaccat
     5581 catcactacc ggcatgatca tcagctgcac caggaacagg agaaacagcg tctgcttgcc
     5641 gcgaaactcg tgacaggcaa agacgtagcc ggcggtggtg atggtcaaca gctgcacgaa
     5701 gaaggtgccg aaggtgaaga taattgtgtt ggcgtacagg ctcagccagt cggcgctgtt
     5761 ccaggcgtcg cggaagttat cgagagtcag cggcaggcgc ggcagcagcg aggccatatc
     5821 gtcgccgaag gtagttgcgc tgaatgaggt ggtcagcatc cagataaacg ggctgaccca
     5881 cagcagcgcc aggcagcaca ttaatactgt gagcgtaaag ggcgacgagc gacgcaggcg
     5941 cagccacagc ggacgcgcag cggcgggtgt gtggaccagc agcggcgaga tgtcagcgct
     6001 catagtgggc tcctttctcc agcagtttca ggttaatcag cgagaaggcg aacagtccgg
     6061 ccagggtcag gaaggtggct gcggaggctt taccaaggtc gtgggtatcc caggtgagat
     6121 tctggatgta atagagcagc acggtggtgg cgttatccgg cccgccgcga gtcatcaccg
     6181 ccacgtggtc aatctgggtg atggagtaga tcagcgcggt ggtgatcaca aagctcagcg
     6241 tcgggcgcag cagcggtagc gtgaccttaa aaaagacctg cgtgcgggta gccccctcca
     6301 tcagcgccgc ttcccgcgtc gaggccggaa tgctctgcaa cccggcgagg aaaaacagca
     6361 tgtagtagcc ggcgaacttc cacacgccga tcagcgccag ggccagcagc gcgctgttgc
     6421 tgcgccccag ccagttgttg ttcatcggcc cgaacagctt tgccagatag tggtcgagca
     6481 ggccgaggcc gggcataaag ataaacagcc acagtgcagc ggcgctgacc atcgggataa
     6541 tcatcgggaa gaagaaggcg gtgcgcagcc aacggttgac gcgatggttc tcgctcaggg
     6601 cgaccgccag cagcagggcg agcgtcaccc cgggcatcac cgtcagcagg atatagagta
     6661 ggttattgcc cagcgactgc cagaagacgt tatcggcaaa caggcgggca aagttatcca
     6721 gcccgacgta ttgcgcgttg gccatgcggg tatcaaacag gctgtcatac accgaacgcc
     6781 ccagcggaaa ccaggtaaac agcagtaaaa acagcaggga aggcgacagg atcagccagg
     6841 gaagccaggt ctttctcatg gttacgcaca tctcgttcag ccatttatcg aagccctaat
     6901 tgtgggaagc tatgattaca gaggtatgac ggggaaacga tggtttgatg aagcaacggg
     6961 gaaaacgccg ggcgagcggg ccgcccggtg gaggagaacc atcataagtc ggctttcagc
     7021 accgcgcggt agcgggcttt gccttcgcga acgtgtttca acgcttcgtt aatttgcgac
     7081 atagggaaca gctcggtggt cggagccact ttgctgcgtc cggcgaactt cctcagcgtg
     7141 cgcagttcgg atgggttacc gatcgccgag ccggagatgc tgcagtcgtc gccgataagg
     7201 gtaaaggccg gtaccgggaa cggcttcatc accgcgtcga cggtgtggaa gttgccgccc
     7261 taggacaggg cttcgaagta cggctgccag tcaagatcca ccgccacggt gttgatgctc
     7321 aggtcaaact gcccggccag cacttttaag gcttcaggat cgcggctatt gactacgcga
     7381 tcggcgccca tcgcccgcag cagcttaatg gcgatatggc cgaggccgcc gatgccgatc
     7441 acccactgcc agtcggcacg cagcttattg gcgaagccgc cgcagcgcgc ttggctgaaa
     7501 aaaatgtcct gctaacgcca tccggcagag catccgccct ggcatttatg atgaccttgc
     7561 gtgccgatcg tcgctataaa gacgctcaac cgcttccctt ggtaacctga cgcagttgcc
     7621 aattgacctg tcaatatgag ttcaccggaa cgacacgaat gaattcttga actcacgcac
     7681 agttcttata aagatgattt cccagt
//
LOCUS       DAPQZD010000204         7645 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPQZD010000204 DAPQZD010000000
VERSION     DAPQZD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.206"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(85..1134)
                     /locus_tag="RO454_004416"
     CDS             complement(85..1134)
                     /locus_tag="RO454_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDY0936146.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQGLIV"
     gene            complement(1286..1483)
                     /locus_tag="RO454_004417"
     CDS             complement(1286..1483)
                     /locus_tag="RO454_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDY0936147.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATAGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1710..2531)
                     /locus_tag="RO454_004418"
     CDS             complement(1710..2531)
                     /locus_tag="RO454_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDY0936148.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCRGEKFIEGEVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKIREVVKVSCPECKGKGEISTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(2528..2908)
                     /locus_tag="RO454_004419"
     CDS             complement(2528..2908)
                     /locus_tag="RO454_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDY0936149.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(2909..3964)
                     /locus_tag="RO454_004420"
     CDS             complement(2909..3964)
                     /locus_tag="RO454_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDY0936150.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(3966..4238)
                     /locus_tag="RO454_004421"
     CDS             complement(3966..4238)
                     /locus_tag="RO454_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936151.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(4406..4561)
                     /gene="hokD"
                     /locus_tag="RO454_004422"
     CDS             complement(4406..4561)
                     /gene="hokD"
                     /locus_tag="RO454_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDY0936152.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRLRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4799..5464)
                     /locus_tag="RO454_004423"
     CDS             complement(4799..5464)
                     /locus_tag="RO454_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDY0936153.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(5639..6064)
                     /locus_tag="RO454_004424"
     CDS             complement(5639..6064)
                     /locus_tag="RO454_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDY0936154.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(6080..6850)
                     /locus_tag="RO454_004425"
     CDS             complement(6080..6850)
                     /locus_tag="RO454_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDY0936155.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(6872..7618)
                     /locus_tag="RO454_004426"
     CDS             complement(6872..7618)
                     /locus_tag="RO454_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDY0936156.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
BASE COUNT         1755 a         2183 c         1738 g         1969 t
ORIGIN      
        1 tgagtcgctc gctctcacca ctgagctaaa gggccgggag cagaataata atggtgcgta
       61 attaattctg caatctcatc cgtttcaaac gattaaaccc tgaacttccc tgactgtctg
      121 ctcaaaacgt ccggtctcca gctcaacgcc aatcgcacga cgcccgagcg ccagtgccgc
      181 ttttaccgtt gaacctgagc ccataaaaaa atctgcaacc aggtcacccg gacgactgct
      241 tgcgctgatt atctgctgca gcatttctgc cggtttttcg cacggatgtt tcccgggata
      301 gaactgcacc ggtttatgtg tccacacatc cgtgtacggc acctgcgccg tcacaccaaa
      361 ataccgccgc agatgcttat attcactctg cagctccaca tactgccggt tcagtgacgt
      421 atacgtatcc accagctggt ggtggggctt ttccagttca ccgcgctgat gtttctcttc
      481 tgccacccgg gcaaacagcg actgtaattt cagataatcg ctttcgttcg gtagctgcca
      541 ctgactggca ctgaaccagt gcgacaccat gtttttcttt cctgtggcat ctgcaatctg
      601 ttttgccgtt atccccaggg ccgcgcgcgc atcacgaaag taagaaatca gcggggccat
      661 cacatgctgt ttcagtgcac tgcccttcgc cgcatacccg gcatctttcg gacgatacgg
      721 cccctgataa tgttccgcga acagaatgcg ctctgtggcg gggaaatacg cccgcaggct
      781 ttccttgttg catccgttcc agcgtccgga cggcttcgcc cagataatat ggttcagcac
      841 actgaagcgt tcacgcatca tgatttcgat atcagatgcc aggcgatgac cacagaacag
      901 gtaaagactt ccggcaggtt tcagcacccg ccagaactgc gccagacact ggtccagcca
      961 cttcaggtaa tcatcgtcgc ccttccactg gttatcccag ccctcaggct tcactttaaa
     1021 gtacggcggg tccgtgacta tcaggtcaac agaattttcg ggtaacgacc ggataaattc
     1081 caggcagtcg gcgttgatta actcacaact ggatattttt acagtattag ccatagatca
     1141 ataagcactt ctctgatagg ctcataccgc ttttgcgcaa agcagatggg cctgaggttt
     1201 gcttgtgacc ccaacgcatg agcagatggc tggtgggtgc ccctaacacc caccagccgc
     1261 ccatttacca caaataaaaa agccttcact gcggaaggcg tctgtaacaa ccgaactgat
     1321 agtctgccag acccgccata accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa
     1381 ggtaagtatt ctgcgcaatc tccccggctg tcgccggttc ggtgacgctt aattcattaa
     1441 acaccactct ggcggtttct gtcatatcct gctgtttcag catgtctttt tcccttttcc
     1501 ggttaacgtg acacaccaat aactcttgtc gaaaaagcca gcaagctgaa agacaggtat
     1561 tcaccgccac cagcgcgttt actgtactga cgcgatttca gtcataaaaa acccgccagg
     1621 cggcggggtg taaaaaatct tctaacgtca ggcataaaac gcccatcgtt agagcaaatt
     1681 taccacagat tcgggaaaaa tcaacaacac tatcgcgtta ccctctttaa ctgccgctcc
     1741 gcccatgcct cttcaatgtc aaaccgaacc accaacgtat cgtaaaagcg tttcactgat
     1801 tttttccacg tatcaagcgt gatagcactc gtcactttgc atatggcatt aaatgcctcc
     1861 gttgatggta gtctttcaca gccacgacca ccacaacgct ggcagtctct gataacaggc
     1921 ataccacgtt ttaccgactc ttcacgatga atggcgacac cacgcccacg gcagtcttta
     1981 caggcagtgg aaatctcccc cttcccttta cattcaggac aagaaacttt caccacctcc
     2041 cgaatttttt tccattcttc ccagtaagac ggatacacac ctttcgtaca ctttgcccat
     2101 actggcggct tgccatccgg atactgaacc ttgtttgtaa aaacttcgcc ttcaataaat
     2161 ttttcccctc ggcaacaggg gcactgcttt ttactcgctg cgctgcgggc ataatcctca
     2221 aaagcgtacg aagccataat gcgcatcact accggtttta tttctgccgg aagttttctc
     2281 aacgccgcca cacgatcgca ccgactgagt gcataatctg ccagtaattc tgttgcccgc
     2341 gccctgtcat tcatactgat gcccattttc cccaggaacg cagaaaaccc catctcagcc
     2401 cgattctgtg tcatgccctg cgcggccatc acatcagtga tactcagcgc atctttcgac
     2461 gttgaggccg atgcatcagt caggccgggg gattttgggg agtagtattt cggtaaatct
     2521 tccagtttca ttttttgacc tgctcttcat gcattatggg gtaaatcttc acccccagac
     2581 gtccaccaga tactggctga ccacgaacga tattgatttc atcaaactgc tcatcgtcca
     2641 ttagcaaccc cgcatgcgtc agcgcatcca gcggcgcttt cagaatattg tccagatcac
     2701 ggcggcgctt atccggtggt tctgcaataa tttttattgc caaccttccg gacaggctta
     2761 atttcagccg ctgctggcga acaataagcg ccactgcccg gcgataacgc tccccggctt
     2821 ttgatacaaa atatgtgctg ccacgacgac gccagtaagt gttcaccgtt ggcgggtaag
     2881 gcaaaacaaa ttctatgcgt tcagtcattt atgctttcca cttcagaaca cccgaatttc
     2941 tcgcgtgcat taaaaaacga atcagcaaca acagctggct gccgtgtttt tcttcaaaat
     3001 cttttacccc ggcgtgtagt tcgctatggc atttacggca cagcggaata acaaacaaat
     3061 catcagcctt tgttcccatc cctcccagtc catgaccaat gatgtgatgc ggatcatctg
     3121 cctgattgcc acacgtcatg catttctgcg tttttaccca acgcgtgtat acaggcatct
     3181 cttcccgttg tgatttctgg cgctggagat actgagccgg tgactccgga tcaacggcaa
     3241 tgctgaccac cgtcttttcc tgtggcgggt tttgctggtg ggcgtgaggc agcggcgcaa
     3301 gattttttgt gcgctgcttc agtatgctgg tggcggtctg ctctcccggt acgatgtcgc
     3361 tttcacggta cattgagcgg attttttccg cacgcaaccc cagcgaacga cgtaataccg
     3421 cttccggtag cgcgtccgcc acctgattgc ggaccgccca ccaggataat tcagccagcg
     3481 ataattcccg ttcctgcgag ccattcattg catggcgtat gacgtcaatc atccatgcaa
     3541 acaggttttg gtgagcaagt tgcccgagtg attcggaggt ctggtcgcgc agctggttgt
     3601 cgcagtgcca gcacaacacc attgcgccgg taccataacg gtgaatgacg gtttcactgt
     3661 ggtgataatc gccgtgtggc cactggcagg atttaacatg gcgcagtaac cagtcagaca
     3721 atgcgccagc gccaccagca gcacgaatca ctcgttcgtc gctgaaaaat ggcagtaatg
     3781 atttatcctc cgccagcggc tggcgaacgg caggaacgac cccggacggc agattacgca
     3841 tgcttttcgg ttccggctcc accagtaccc gggtattgtg gaataccggc atggattcac
     3901 ggcccggctt aacgatcacc agcccgagtt ccggtaccag aacaggtcga agtaataccc
     3961 gcacgttacc tccagatgcg ttgctggaat gtgcgggacg gacgcggtgg gcgttcagag
     4021 taaggaagcc tgacggagat tatccagtga cgataatcga ggctgagggc tttcttaatc
     4081 tcgtatccgc gtctgcggta gttatgaatt agccattcgg cctgttcttc agtacatggt
     4141 gggtgttggt accagtcggt tttaaatgcg tgtgaacgcc gcccatgccg gatggcaagg
     4201 tcggtatcag aattgtgaaa tttggttttg tgcgccatct gttttctctg ctggcgcagc
     4261 aggtgtcagg tgttcaggct gacgtgcgaa ttgtaaacca gaatgccaga aaaaaacaaa
     4321 acccgccgaa ccgggttaag tgcgggtgcg ttgaggatgc ctgacacatc agaggtggcg
     4381 agggatttct cccccgccgg gtctcttact cctcaggttc gtaagctgtg aagacagcga
     4441 cctccgtctg gccggttcgg agtcgtacct cgcagaggtc tttcctcgtt accagtgccg
     4501 tcactatgac ggttaaacag atgacgatca gggcgattaa catcgccttt tgctgcttca
     4561 tagcctgctt ccccttgcct ttcggcacgt aagaggctaa cctacatttg tgagacatag
     4621 attgggcctc agattaatgt taagcgtctt gcaggacgcg aaatgttaac tggggctttt
     4681 ctctatctgc ctttcagtgt tcatgcctga gacagatagc ctcaagcacc cgcagccatt
     4741 ctacttaact cacgtcacct cgccaatatg aaatcaatca gaaaggtgat ccataaaatc
     4801 actccttctc ttcttttccg tagtggagtt ggccaatttt gataagaggg cgtccctgag
     4861 atttgcggtg tagattggta tcgcgcagag aatacacaca gccacaatat tcctgctgat
     4921 agaatttttc gcgcttgctg atttcaatca tacgggacga gccgccctgc ttgcgccagt
     4981 tataatccca gtacaccata cccggataat gcgcaacagc tcgccgccca cactcgttaa
     5041 cctgctgcat atttttccag cgtgaaatgc ccagtgaact gctgatcaca ctgaaaccat
     5101 tttcagcagc gtacaacgct gtccgctcaa aacgcatgtc aaaacacatg gtacaacgga
     5161 tccccctctc aggctcccat tccattcctt tggcacgttc aaaccagttg tcggtgtcgt
     5221 aatcagcatc gataaacggc acgccgtgtt gttcagcaaa gcgaatattt tcatccttac
     5281 gaattaaata ctctttctga ggatgaatgt tcgggttgta gaaaaagatg gtgtagtcga
     5341 ttcccgaggc ctgaagcgcc tccatcactt caccggaaca tggagcacag caagagtgca
     5401 gtagtagttt gtttgccccg tttgggagct ccaatttagg ccgtttgaaa tcagcaatag
     5461 tcataaatat ttttattggg gtcatgaaaa tagcacagag tgtagcatca gagcagggct
     5521 atcgggaata tatgtctaaa tctggtaata tctggttttg acgcaaagcg gacaaccacg
     5581 ctggctctac cctgcgccat gaaaatgtca attcacatct gaactaatgc tctttaatct
     5641 agtaacgtct aaaataccta acatttcctt gataaaatgc cagtacacgc tgcatagctt
     5701 cgctcttccg gcactcgcga cagattatat tcaggcgcct gtcgtagcgg cgtatttcgc
     5761 cgtctggtaa cgaccagata aggtccggat caaccactgc aggtttcttc acctttgccc
     5821 ttgagagttt tttgcgagca ttttgccagt ccttacgcgc ctgttcagac gggaataacc
     5881 cgtaaccaga gttgtataca tcgccactgg caaccagctc tctggcgaga acactcatca
     5941 gatatcttgt cgcacctgtc ttggcttcca gttgccgcaa cgtctcgcga ccgctcagac
     6001 gtacaagttc aacaacctgc cctttaattt tttcccgctc ttcttgtgta aatacttttg
     6061 ccataagcgc ctccggcaat cacttttccg atacaacacg gcgggaagaa tcagtaatct
     6121 gtcgaacaat atcccggtgc ttgttcagct cccgcagcgc ggcgcagact cgctcccact
     6181 tctgaacatc acttttcgcc ctgcgcagcg ccaggtttgc cctgcgaagg gacggaaaaa
     6241 tcagctcatc tgcttgcgtt tcggtaaacg atggcaacgg ctgcacaatg tccgccacag
     6301 tttctgtttt aatttcttcc tgtgttgcgg cttcccggac tggtaacgca gcacctgctg
     6361 gctgaggaaa ggccttacca tcactttccg ttaccagcgc ggctttcggc tctgctggta
     6421 aattatcgcc cggcatgcag taacgaaatt taccgttctg attaacgcgt gccagccgcc
     6481 ccgttgcggt taccaccgcc agcgtggaag caaccttgcg agtactgaca ccgaacttac
     6541 ccgccagttc ctcacacgtt ttagccccat cctgaccgat aaactcaatc atcatgtctg
     6601 cggtaacttt ttgttcgacc tccccggtca gcatatcctg tgcttcagat tttactggcc
     6661 gctcttcggt tacccgggat tcaccttcgc cagccagaaa ccaggtgtga ccagttttat
     6721 caacgacgcc ttttcttttg agttcccaca gctcgttgac agcctcttca cgactgattc
     6781 caaggcgagc tgccaccaca tgtgaagagg cttttttcag tgctttcagt gcgtcagata
     6841 cggtttccat taaaatttcc tccggacaaa attacttcac aaccctcata ttgctgacat
     6901 ttggacgcca gctatcccag ttaaacgtca cccatcgacc accgttcatg gtcatgcggt
     6961 ccataatcct ctcaccaaga agcgtactca ttgcggcatg attcaggttt gttaacatcc
     7021 cgacactgca cagtgatgct gtccggcgat caattatctg gtgcaatacc acctgctcgt
     7081 ttttcgtctc ccgctgaacg cctatttcat ccaggaccag caaatcaacc ccgcaaagct
     7141 cctgtaaaaa tttttccccg gatttgccgt tgtcgtagct gtcatgcaac acgctcatga
     7201 cgtcagacac ggtgacgata atcacgctgc gccccttcac catcagccgg ttgcccatcg
     7261 ccgctgcaag gtgatttttc ccggtgccgg ttttaccgct gaacacaaaa ttcgtgcacc
     7321 cggtcatcag ttcgtcagct atggatttgg cctggctcag cgcgtatttt tgcccgtcgt
     7381 tctgcacctg ataatttgca aacgagcatt tgctgtgcag aggctggatg cccgaacgat
     7441 tcaggatttt ttccacccgc aactggcgat tctggcggtt aatctcctcg ctgcgttttc
     7501 gtccttcagc aagttgccat tcccgccact cctccaccgt ccggtacggt ggaaccgacc
     7561 cctgtggtgc aagtctgcga atacgttcaa gaaccccaac tgccgcaatg tttttcatga
     7621 cacgtcaccc cctgaatccc ggcgg
//
LOCUS       DAPQZD010000205         7476 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPQZD010000205 DAPQZD010000000
VERSION     DAPQZD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.207"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            172..4419
                     /gene="fdeC"
                     /locus_tag="RO454_004427"
     CDS             172..4419
                     /gene="fdeC"
                     /locus_tag="RO454_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDY0936157.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWIAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4544..5401)
                     /locus_tag="RO454_004428"
     CDS             complement(4544..5401)
                     /locus_tag="RO454_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0936158.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5649..6518
                     /locus_tag="RO454_004429"
     CDS             5649..6518
                     /locus_tag="RO454_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDY0936159.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6678..7271)
                     /gene="rclC"
                     /locus_tag="RO454_004430"
     CDS             complement(6678..7271)
                     /gene="rclC"
                     /locus_tag="RO454_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDY0936160.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7283..>7476)
                     /locus_tag="RO454_004431"
     CDS             complement(7283..>7476)
                     /locus_tag="RO454_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0936161.1"
                     /translation="MAFSTNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSW
                     KVTSMRIDNNSTATAVLYK"
BASE COUNT         2051 a         1660 c         1922 g         1843 t
ORIGIN      
        1 atgagtggct acagacactg gatatattag gtggcggtgt actttcttac ataaaggtat
       61 ttccttttct gtggaaaagg aaaccgggaa atccccggtt tttctgacaa gcagacgcca
      121 ttatttgtgt ctgcctatgt tcgttaattc gttcatcagg aaattatctc aatgtcacgt
      181 tataaaacag acaataaaca gccacgattt cgttattcag ttctggcccg ctgcgtggcg
      241 tgggcaaata tctctgttca ggttcttttt ccactcgcag tcacctttac gccagtaatg
      301 gcagcacgtg cgcagcatgc ggttcagcca cggttgagca tggaaaatac tacggtaact
      361 gctaataata acgtggagaa aaatgtcgcg tcgcttgccg ctaatgccgg gacattttta
      421 agcagtcagc cagatagcga tgcgacacgt aactttatta ccggaatggc caccgctaaa
      481 gctaaccagg aaattcagga gtggctcggg aaatacggta ctgcgcgcgt caaactgaat
      541 gtcgataaaa atttctcgct gaaggactct tcgctggaaa tgctttatcc gatttatgat
      601 acaccgacaa atatgttgtt cactcagggg gcaatacatc gtaccgacga tcgtactcag
      661 tcaaatattg gttttggctg gcgtcatttt tcagaaaatg actggatggc gggggtgaat
      721 acttttatcg atcatgattt atcccgtagt catacccgca ttggtgttgg tgcggaatac
      781 tggcgtgatt atttgaaact gagcgccaat ggttatatcc gggcttctgg ctggaaaaaa
      841 tcgccggatg ttgaggatta tcaggaacgc ccggcgaatg gctgggatat tcgtgctgag
      901 ggctatttac ctgcctggcc gcagcttggc gcaagcctca tgtatgaaca gtattatggc
      961 gatgaagtcg ggctgtttgg taaagataaa cgccagaaag atccacatgc gattaccgct
     1021 gaagtgaatt acacgccagt gcctcttctg accctgagtg ccgggcataa gcagggcaag
     1081 agtggtgaga atgacactcg ctttggcctg gaagttaatt atcggattgg cgaacctctg
     1141 gaaaaacaac tcgatacaga cagcattcgc gagcgtcgaa tgctggcagg cagccgctat
     1201 gacctggttg agcgtaataa caatatcgtt cttgagtatc gcaaatctga agtgatccgt
     1261 attgctctgc ctgagcgtat tgaaggcaag ggcggccaga cggtttccct ggggcttgtg
     1321 gtcagcaaag caactcacgg tctgaaaaat gtgcaatggg aagcgccgtc tttgctggcc
     1381 gcaggcggaa aaattacggg gcagggcaat cagtggcaag tgacgctccc ggcttatcag
     1441 gcaggcaaag acaattatta tgcgatttca gcgattgcct acgataacaa aggcaatgcc
     1501 tcgaaacgtg tgcagacaga agtggttatt agcggagctg gtatgagcgc cgatcgtacg
     1561 gcgttaacgc ttgacggtca gagccgtatt caaatgcttg ctaacggtaa tgagcaaaag
     1621 ccgctggtgc tgtctctgcg cgacgccgag ggtcagccag tcacgggcat gaaagatcag
     1681 atcaagactg aactaacctt caaaccggct ggaaatattg tgactcgtac cctgaaggcc
     1741 actaaatcac aggcaaagcc aacactgggt gagttcaccg aaactgaatc aggggtgtat
     1801 cagtctgtct ttactaccgg aacgcagtca ggtgaggcaa cgattactgt tagcgttgat
     1861 gacatgagca aaactgtcac tgcagaactg cgggccacga tgatggatgt ggcaaactcc
     1921 accctgagcg ctaacgagcc gtcaggtgat gtggttgctg atggtcagca agcctacacg
     1981 ctgacactga cagcggtgga ctccgagggt aatccggtga cgggagaagc cagccgcctg
     2041 cgacttgttc cgcaagacac taatggtgta accgttggtg ccatttcgga aataaaacca
     2101 ggggtttaca gcgccacggt ttcttcgacc cgtgccggga acgttgttgt gcgtgccttc
     2161 agcgagcagt atcagctggg cacgttacaa caaacgctga agtttgttgc cgggccgctt
     2221 gatgcagcac attcgtccat caccctgaat cctgataaac cggtggttgg cggtacagtt
     2281 acggcaatct ggacggcaaa agatgctaat gacaaccctg taactggcct caatccggat
     2341 gcaccgtcat tatcgggcgc agctgctgct ggttctacgg catcaggctg gacggataat
     2401 ggcgacggga cctggattgc gcagatttct ctcggcacta cggcgggtga attagacgtt
     2461 atgccgaagc tcaatgggca ggacgcggca gcgaatgcgg caaaagtaac cgtggtggct
     2521 gatgcattat cttcaaacca gtcgaaagtc tctgtcgcag aagatcacgt aaaagccggt
     2581 gaaagcacaa ccgtaacgct ggtggcgaaa gatgcgcatg gcaacgctat cagtggtctt
     2641 tcgttgtcgg caagtttgac ggggaccgcc tctgaagggg cgaccgtttc cagttggacc
     2701 gaaaaaggtg acggttccta tgttgctacg ttaactacag gcggaaagac gggcgagctt
     2761 cgtgtcatgc cgctcttcaa cggccagcct gcagccaccg aagccgcgca gctgactgtt
     2821 attgccggag agatgtcatc agcgaactct acgcttgttg cggacaataa aactccaacg
     2881 gttaaaacga cgacggaact caccttcacc gtgaaggatg cgtacgggaa tccggtcacc
     2941 gggctgaagc cagatgcacc agtgtttagt ggtgccgcca gcacggggag tgagcgtcct
     3001 tcagcaggaa actggacaga gaaaggtaat ggggtctacg tgtcgacctt aacgctggga
     3061 tctgccgcgg gtcagttgtc tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag
     3121 ccactggtgc tgaatgttgc aggtgacgca tctaaggctg agattcgtga tatgacagtg
     3181 aaggttaata accaactggc taatggacag tctgctaacc agataaccct gaccgtcgtg
     3241 gacagctatg gtaacccgtt gcaggggcaa gaagttacgc tgactttacc gcagggtgtg
     3301 accagcaaga cggggaatac agtaacaacc aatgcggcag ggaaagtgga cattgagctt
     3361 atgtcaacgg ttgcgggaga acacaatatt tccgcttcgg tgaatggtgc tcagaagacg
     3421 gtcacggtga aattcaacgc ggatgccagc accggtcagg caaacctgca ggtagacacc
     3481 gccgttcaaa aagtggcaaa cggcaaagat gcctttacgc tgacggcgaa cgttgaggat
     3541 aaaaatggta accctgttcc agggagcctg gtgaccttta atctgccccg gggtgtcaag
     3601 ccgcttacag gcgataatgt gtgggtgaaa gccaacgatg aggggaaagc ggagttgcag
     3661 gtggtttccg tgactgccgg aacctatgag atcacggcgt cagcaggaaa tgaccagcct
     3721 tcgaatgcgc agtctgtaac gtttgtggcc gataagacta cggcgaccat ctccagtatt
     3781 gaggtgattg gcaaccgtgc agtggcggac ggcaaaacca aacagacgta taaagttacg
     3841 gtgactgatg ccaataacaa cctgctgaaa gatagcgaag tgacgctgac tgccagcccg
     3901 gaaaatttag ttctgactcc caatgggacg gcgacaacga atgagcaagg gcaggctatt
     3961 ttcaccgcca cgaccactgt cgcagcgaca tatacactca cggcgaaagt ggaacaggcc
     4021 gacggtcagg aatcgacgaa aactgccgaa tctaaattcg tcgcggatga taaaaacgcg
     4081 gagctcgccg caacatctga tgtgcattct ctggtggcgg atggggtaac gacagcaaca
     4141 ctgacggtaa ccctgttctc ggcaaacaac ccggtaggag gaaccatgtg ggtcgacatt
     4201 gaggctccgg aaggggtgac agaggcggat tatcagttcc tgccgtcgaa aaatgaccat
     4261 ttcgcgagcg ggaaaatcac gcgtacattt agtaccaaca agccaggtac atacacattc
     4321 acattcaact ctttgacata tggagggtat gaaatgaaac ccgtgactgt gacaattaac
     4381 gccgttcctg cagatactga aggtgctgag gagaaataaa aacgacgatg taataaataa
     4441 taatggcgtt gacatgcgca gcgccattat ttactgaata cctaccctgg caccgattag
     4501 acggtggcag ggtagggtaa atcttatgtg atggagttac aggctaaacc gggcgaacgg
     4561 ggataaaaat ttcgcaaaca atgttattgc tatcaatttc gacgaagttt tcattgtaat
     4621 aaaatcgctc aatatcactg ccgttcactc tggctaaccc cctggcaggt aattccatca
     4681 gatagaggtt tcgcgcccat tcagggtagt gctcccactc accttcatag cggaaagagg
     4741 catacagtcc tcggttaatt gtccactctt tcggcatctc tgaatcggtt tgtttgcata
     4801 ccgtaccggc aaaagtttca actgtgagca aatcgaccga ttttgttgat ggttcgaacg
     4861 tggagacgaa atagatatcc gtcttaaaat tcttcataca tctgactaaa tgatgacgtc
     4921 gcagtttgac ttcttcatcc ggtaatttat tcgacacttc aatatcatat ttaaatgaat
     4981 tgccaaaaat aggtgtctca ggaaagttga ctaatctaca ctccgtcttt tgctgttgac
     5041 gtattaaaaa agaagggaag atatttgcga gatcccaata atcacggtgg cggtattcac
     5101 ggggagagca gccgaataac tttttaaatt cgcggctgaa tgactgctgt gaatcaaaat
     5161 gcaaagagag tgcaatatca agcatagatt tcgcggagag ccggacaaga atggcggcac
     5221 gacaaagcct tcgtttgcga atgtattctc ccaaaggcac atgcatgaaa tttcggaaca
     5281 gaagctggat gttgcgtctg ctgtagccag atttctgtgc gatatcttcg attgaaatag
     5341 ggtgctcaag attgcactca atccactcca ggagctgctg tagaatcttc tgcctgatca
     5401 tcacatactc cgttaattct ttgtcatatc gcacattttc gttatgtcct gggccctaag
     5461 tgtatcaaaa acagcaagtc ccaaggcaaa agcactgata gcgtctgctt cattgcccca
     5521 aaaatagaga ggtttcagtc ctacattatt aatgaatttt ttgcataagt gatatcaaaa
     5581 tccacatact aatttgaggt tacgttttaa cgtagactca ttgttcatac ctaatggagg
     5641 gactgacagt ggaattttcc gtattaagta acaatctgaa aatgccgatg atgggatttg
     5701 gtgtttttca ggttaccgat aaagacgtgt gcaaacagtc agtgttgaat gctatccgca
     5761 cgggttatcg actcatcgac actgcagcgg tatacggtaa tgaagatgcc gtcggtgaag
     5821 ccgttcgtga agccatatct gaaggtttat gtacccgaga agagttattt ataacgtcta
     5881 aattgtgggt gcaggatatg ttgaatcagg atattgcagc agcaggtatt gaagcatcat
     5941 taaaaaaatc ggggctagaa tacttcgatc tctatttatt gcaccaggcc atgcgcgatt
     6001 attttagtgc gtggcgtgca ctcgaagatg cctatgaaga gggcaaatta aaagcgattg
     6061 gggtttccaa tttctaccct catgttctgg cgaacttttg tgaaacggta agagttaaac
     6121 cgatggtcaa ccaggtcgag ttgcatccct attttgccca accagaggcg ctggcaacca
     6181 tgaagtatta taacgtgcag cctgaagcat gggctccgtt aggtggtgga cgacataaac
     6241 cctttgaaaa taatctgctt cagagtattg cagatgccca tcaaaaatcg atttctcagg
     6301 tcattctgcg ttggaatatt caacggggag tggtcgttat tccgaaatcg acacatcaac
     6361 agcgtatcga agaaaatttt gctatctggg atttctcact gacagagaaa gaaatggcgc
     6421 aaattagttc gctagatttg ggttatgttg gggaatcggt aaaacatttt aatcctgagt
     6481 ttgttcgtgg ttgtcttgct gtaaaaatac atgattgata ttaatcactt attttacctg
     6541 agacgacaaa aattttttaa caggggagtg atattgatcg tcactctgtc atatctccgg
     6601 taatatggcg tcaggcttca tcaacagaaa gggggcctga cgcctgaaaa agtgaacaac
     6661 agacagtgtt cggattatca atattcagtt tttaacgttg aactggattc attactgcgt
     6721 tgtttaagaa tatcccgtgc cgaatctgcc attatcatga cagcacctgc cagcatcaga
     6781 gtatctttca ataccagacg accagcaccg gataaataag ggaaaccatg atgagcatca
     6841 cccaatgcgg gtacccatgc ctccggagtg gtgattaaaa atgagagtgt taccagcggc
     6901 gtggtaaatg ccatcagccc acccaataaa cctaaccagc gattgacagg attagccaaa
     6961 accagcagag caataatcac ctccacgacg ccaagaccgt tggaaaaacc ataggtatta
     7021 ttagccgttt gccatgctct tgcttctggt ttgtattcgc cttcgtgagt cagatactgt
     7081 ttatagtctt ccgggtgttc ataaaagaac gacattagtg gactgtttgc gacgaatggt
     7141 gtaatgctgt ctgcctcgta agggacaaac tttaataacc caatccacat aaaaacaatc
     7201 gcaatgctca gacgaatcaa tgtcaggcca attttatctc cccgacttaa caggtgcagg
     7261 tatctttcca ttgtaaacat ccttatttat acaatactgc tgttgcggtt gaattattat
     7321 caattcgcat cgatgtaact ttccaggatg aagcattata ttttctggcc atatcagcaa
     7381 tggcattctc aagaactgtc ggtgatgctg atactgatga gacgctgata tgtttcagaa
     7441 ttattttatc atctgcattg gtggaaaatg ccataa
//
LOCUS       DAPQZD010000206         7433 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPQZD010000206 DAPQZD010000000
VERSION     DAPQZD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.208"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            9..224
                     /gene="ydgT"
                     /locus_tag="RO454_004432"
     CDS             9..224
                     /gene="ydgT"
                     /locus_tag="RO454_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDY0936162.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            310..750
                     /locus_tag="RO454_004433"
     CDS             310..750
                     /locus_tag="RO454_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDY0936163.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            827..1408
                     /gene="rsxA"
                     /locus_tag="RO454_004434"
     CDS             827..1408
                     /gene="rsxA"
                     /locus_tag="RO454_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDY0936164.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1408..1986
                     /gene="rsxB"
                     /locus_tag="RO454_004435"
     CDS             1408..1986
                     /gene="rsxB"
                     /locus_tag="RO454_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDY0936165.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            1979..4399
                     /gene="rsxC"
                     /locus_tag="RO454_004436"
     CDS             1979..4399
                     /gene="rsxC"
                     /locus_tag="RO454_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDY0936166.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANA
                     EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAA
                     QQKVVNED"
     gene            4400..5458
                     /gene="rsxD"
                     /locus_tag="RO454_004437"
     CDS             4400..5458
                     /gene="rsxD"
                     /locus_tag="RO454_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDY0936167.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5462..6082
                     /gene="rsxG"
                     /locus_tag="RO454_004438"
     CDS             5462..6082
                     /gene="rsxG"
                     /locus_tag="RO454_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDY0936168.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            6086..6781
                     /gene="rsxE"
                     /locus_tag="RO454_004439"
     CDS             6086..6781
                     /gene="rsxE"
                     /locus_tag="RO454_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDY0936169.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            6781..7416
                     /gene="nth"
                     /locus_tag="RO454_004440"
     CDS             6781..7416
                     /gene="nth"
                     /locus_tag="RO454_004440"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDY0936170.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1725 a         1930 c         1991 g         1787 t
ORIGIN      
        1 gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt tcactcgaaa
       61 gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag gaactgatca
      121 atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg cgtttgtttg
      181 acctcggcca ggtaccgaag tccgtctggc actatgtcca gtaaagaaag tagcgatcgg
      241 ttataaaagc cttataataa cgcccctaaa attagatttg tctgtttgcg tcgctattct
      301 ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct tggcccgttg
      361 gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta taccgctgcg
      421 ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac acaaatctta
      481 tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg gttgaccatc
      541 gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct gcttatttcc
      601 gtattgctgg cagtaaaagc gttcgctttt tcacccgtag aagatggcat agccgttcgc
      661 cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa acgctcgtcg
      721 cgggtgaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc
      781 tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga ctgactacct
      841 gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt ttctcggtct
      901 ctgtccgttt atgggggttt ccaaaaagct ggaaaccgcg atgggcatgg ggctggcaac
      961 aacgtttgta atgacgctgg cgtctatttg cgcctggctt atcgatacgt ggattttgat
     1021 cccacttaat ctgatttatc tgcgcaccct ggcgtttatt ctggtgattg ctgtggttgt
     1081 gcagttcacc gaaatggtgg tgcgcaaaac cagcccggta ctttaccgtc tgctggggat
     1141 ttttttgccg cttatcacca ccaactgtgc cgtgctcggc gtggcgttgc tgaatatcaa
     1201 tctcgggcac aatttcttgc agtcagcgct gtacggtttt tccgccgctg tcggtttctc
     1261 gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg atgtcccggc
     1321 accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc tggcctttat
     1381 gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt tgccgccgtg
     1441 agcctgctgg ccctggcgtt tggcgccatt ctgggttatg cctcccgccg ttttgcggtg
     1501 gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca gtgtggtcag
     1561 tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc
     1621 aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc
     1681 gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc cagcgcggat ggtggcggtt
     1741 attgatgaaa ataactgtat tggctgcact aaatgtattc aggcgtgtcc ggtagacgcc
     1801 atcgttggcg ctacccgcgc catgcatacg gtaatgagtg atctctgtac gggctgcaat
     1861 ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc agaaactcct
     1921 gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt ggaacaccat
     1981 gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca
     2041 tccaccggag atgaaaaccc agtccaacgg tacgcccctg cgccaggtac cactggcgca
     2101 gcgttttgtc attccactaa aacaacatat tggcgctgaa ggtgagttgt gcgttagcgt
     2161 cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa tgctgccggt
     2221 tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg ctcatccttc
     2281 agctttagct gaattaagtg tgattattga tgccgatggc gaagactgct ggatcccgcg
     2341 cgacggctgg gccgattatc gctctcgcag tcgcgaagag ttaatcgagc gcattcatca
     2401 gtttggtgtt gccgggctgg gtggtgcggg atttccgaca ggcgttaaat tgcagggcgg
     2461 cggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt acattaccgc
     2521 cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtatcc gcatccttgc
     2581 gcatattctg cagccgcgcg aaattcttat cggcattgaa gataacaaac cgcaggcgat
     2641 ttccatgcta cgcgcggtgc tggcggactc ccacgatatt tctctgcggg tgattcccac
     2701 caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga agcaggttcc
     2761 acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca ccgcttatgc
     2821 agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgtcgtga ccctgactgg
     2881 cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggtacgccag tgcgtcattt
     2941 attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg gcggcccgct
     3001 aatgggcttt accttaccgt ggctggatgt cccggtcgta aagattacca actgtctttt
     3061 ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagttgca tccgttgtag
     3121 cgcctgtgct gacgcctgcc ctgcggatct tttgccgcaa cagctgtact ggttcagcaa
     3181 aggtcagcaa cacgataaag ccaccacgca taacattgcc gactgcattg aatgtggggc
     3241 ttgcgcgtgg gtttgcccga gcaatatccc cctggtgcaa tatttccgtc aggaaaaagc
     3301 tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga
     3361 agcgcgccag gctcgtctgg agcgcgaaaa aacggctcgc cttgaacgac ataagagcgc
     3421 agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg cgcgggtgaa
     3481 agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac gcccggataa
     3541 cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga aacaggcaga
     3601 actgcagcaa actaacgacg ctgcaaccgt tgctgatcca cgtaaaactg ccgttgaagc
     3661 ggctattgcc cgcgccaaag cacgcaagct ggagcagcaa caggccaatg cggagccaga
     3721 acaacaggtt gatccgcgca aagccgccgt cgaagccgct attgcccgtg ccaaagcacg
     3781 caagctggaa cagcaacagg ctaatgccga accagaacaa caggtcgatc cgcgcaaagc
     3841 tgccgtcgaa gcggctattg cacgcgccaa agcgcgcaag ctggaacagc aacaggccaa
     3901 tgcggaacca gaagaacagg ttgatccacg caaagccgcc gtcgaagctg ctattgcccg
     3961 tgccaaagca cgcaagctgg aacagcaaca acaggctaat gcggaaccag aagaacaggt
     4021 tgatccacgc aaagccgccg tcgaagccgc tatcgcccgc gccaaagcac gcaagctgga
     4081 acagcaacag gccaatgcgg agccagaaca acaggtcgat ccgcgcaaag ctgccgtcga
     4141 agcggctatt gcacgcgcca aagcgcgcaa gctggaacag caacaacagg ctaatgcgga
     4201 accagaagaa caggttgatc cacgcaaagc cgccgtcgaa gctgctattg cccgtgccaa
     4261 agcacgcaag ctggaacagc aacaggctaa tgcagagcca gaagaacaga ttgatccgcg
     4321 caaagcggca gttgctgcgg ctattgcccg cgttcaggcc aaaaaagccg cccagcaaaa
     4381 ggttgtaaac gaggactaaa tggtattcag aatagctagc tccccttata cccataacca
     4441 gcgccagaca tcgcgcatta tgatgttggt attgctcgca gccgtgccag gaatcgcagc
     4501 gcaactgtgg ttttttggtt ggggtactct cgttcagatc ctgttggcgt cggtcagtgc
     4561 tcttctggcc gaagctctcg tcctcaaact acgcaagcag tcggtagctg caacgttgaa
     4621 agataactca gcattgctga caggcttatt gctggcggta agtattcccc ccctcgcgcc
     4681 atggtggatg gtcgtgctgg gtacggtgtt tgcggtgatc atcgctaaac agttgtatgg
     4741 cggtctgggg caaaacccgt ttaatccggc aatgattggt tatgtggtct tactgatctc
     4801 cttcccggtg cagatgacca gctggttacc gccacatgaa attgcggtca acatccctgg
     4861 ttttatcgac gccatacagg ttattttcag cggacatacc accagtggtg gtgatatgaa
     4921 cacgctacgc ttaggtattg atggcattag tcaggcgaca ccgctggata catttaaaac
     4981 ctctgtccgt gccggtcatt cggttgaaga gattatgcaa tatccgatct acagcggtat
     5041 tctggcgggc gctggttggc aatgggtaaa tctcgcctgg ctgactggcg gcgtgtggtt
     5101 gctatggcag aaagcgattc gctggcatat tcccctcagc ttcttagtaa cgctggcgtt
     5161 atgcgcaacg ttgggttggt tgttctcacc agatactctg gcagcaccgc aaattcatct
     5221 gctgtctggt gcgaccatgc tcggcgcatt ctttattttg actgacccgg ttaccgcttc
     5281 tacgaccaat cgtggtcgtc ttattttcgg cgcgctggcg ggcttattag tctggttgat
     5341 ccgcagtttc ggcggctatc ctgacggcgt ggcttttgcc gtcctgctgg cgaacatcac
     5401 ggttcctctg atcgattact acacgcgtcc gcgcgtctac ggccatcgca aagggtaaac
     5461 catgctgaaa actatccgaa aacacggcat tacgttggcg ctctttgcag cgggttcaac
     5521 agggttaact gcggccatca accagatgac caaaacgaca attgctgagc aggccagtct
     5581 gcaacaaaag gcgttatttg atcaggtgct gccagctgaa cgctataaca atgcgctggc
     5641 acagagttgc tatctggtaa ctgcgccaga gttaggtaaa ggtgagcatc gggtttacat
     5701 cgccaaacag gatgacaaac cggtagctgc cgttctggaa gcaaccgcgc cagatggcta
     5761 ttccggtgcg attcagctgc tggtgggagc cgattttaac ggcacggtac ttggcacgcg
     5821 cgtgactgag caccacgaaa cgccagggct tggcgataaa atcgaactgc gcctttctga
     5881 ctggatcacc cattttgcgg gtaaaaaaat cagtggtgcg gatgatgcga actgggcggt
     5941 gaagaaagat ggtggtgatt tcgaccagtt cactggcgcg acgattactc cccgcgcggt
     6001 ggttaatgcg gtaaaacgcg ccggattgta cgctcagacg ttaccggcac aactttctca
     6061 acttcctgcc tgtggagaat aaaacgtgag cgaaattaaa gacgttattg ttcaggggtt
     6121 gtggaaaaac aactctgcgc tggtccagct gctcggcctt tgtcctctgt tggcggtcac
     6181 gtccactgcc actaacgctc tgggtttagg acttgcgact acgctggtac tgacgctaac
     6241 caacctgacc atttcgacgc tgcgtcactg gacgcccgcc gagatccgca ttcccattta
     6301 cgtgatgatc atcgcctcgg tggtcagcgc tgtacagatg ctgatcaacg cctacgcctt
     6361 tggcctgtat caatcattag ggatttttat tccgctgatt gtcactaact gtatcgttgt
     6421 gggccgcgct gaagccttcg ccgccaaaaa aggtccggcg ctttcggcac tggacggctt
     6481 ttcaattggt atgggcgcaa cctgcgccat gttcgtgctg ggttcactac gcgaaattat
     6541 cggcaatggc acgttgtttg acggtgcaga tgcgctgtta ggtagctggg caaaagtatt
     6601 acgcgtggag attttccaca ccgactcccc tttcctgctg gcgatgctgc caccaggtgc
     6661 atttattggc ctgggactga tgctggcagg aaaatacctg attgatgaaa aaatgaaaaa
     6721 gcgccgtact gaagcagctg ccgaacgtgc attgccaaac ggtgaaacag ggaatgtctg
     6781 atgaacaaag caaaacgcct ggagatcctc actcgcctgc gtgagaacaa tcctcatccc
     6841 accaccgagc ttaatttcag ttcgcctttt gaattgctga ttgccgtact gctttccgct
     6901 caggcgaccg atgtcagtgt taataaggcg acggcgaaac tctacccggt ggctaatacg
     6961 cctgcagcga tgcttgaact gggcgttgaa ggggtgaaaa cctatatcaa aacgattggc
     7021 ctttataaca gcaaagcaga aaatatcatc aaaacctgcc gtatattact ggaacaacat
     7081 aatggcgagg ttccggaaga tcgtgctgcg cttgaagccc tgcctggtgt aggtcgtaaa
     7141 acagccaacg tcgtattaaa cactgcatac ggctggccga ctattgctgt cgacacgcac
     7201 attttccgcg tttgcaatcg tactcaattt gcgccgggga aaaacgtcga acaggtagaa
     7261 gaaaagctac tgaaagtggt tccggcagag tttaaagtcg actgccacca ttggttgatc
     7321 ctgcacgggc gttatacctg cattgcccgc aagcctcgct gtggctcttg tattattgaa
     7381 gatctttgtg aatacaaaga gaaagttgac atctaaagat caggggtaac acc
//
LOCUS       DAPQZD010000207         7425 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPQZD010000207 DAPQZD010000000
VERSION     DAPQZD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.209"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            214..2364
                     /gene="btsT"
                     /locus_tag="RO454_004441"
     CDS             214..2364
                     /gene="btsT"
                     /locus_tag="RO454_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDY0936171.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2414..2617
                     /locus_tag="RO454_004442"
     CDS             2414..2617
                     /locus_tag="RO454_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDY0936172.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2628..3584
                     /gene="yjiA"
                     /locus_tag="RO454_004443"
     CDS             2628..3584
                     /gene="yjiA"
                     /locus_tag="RO454_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDY0936173.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVISTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3618..3920)
                     /locus_tag="RO454_004444"
     CDS             complement(3618..3920)
                     /locus_tag="RO454_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDY0936174.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(3907..4194)
                     /locus_tag="RO454_004445"
     CDS             complement(3907..4194)
                     /locus_tag="RO454_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDY0936175.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIISARKADRKERSRYEHG"
     gene            4391..5557
                     /locus_tag="RO454_004446"
     CDS             4391..5557
                     /locus_tag="RO454_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDY0936176.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIRDQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRGMVTREGMSYCITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            5621..7240
                     /locus_tag="RO454_004447"
     CDS             5621..7240
                     /locus_tag="RO454_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDY0936177.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTEDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            7230..>7425
                     /locus_tag="RO454_004448"
     CDS             7230..>7425
                     /locus_tag="RO454_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDY0936178.1"
                     /translation="MGSKWCIYRLGDIAEFSYGKMPKKEFVGTGDYVIFSGYKYTESY
                     PEKNTSAGELIVVARGVGGTG"
BASE COUNT         1805 a         1930 c         1932 g         1758 t
ORIGIN      
        1 atatttatcg tcacttaaac gacgcctttg ccgctcaacc gcaaaactga ccgcttacat
       61 ccctaaaata accactcagt tatttacctt actttacgcg cgcgtaactc tggcaacatc
      121 actacaggat agcggtcaat ttacctcctc aaacgcaacg caaacctaga acggcttcgg
      181 ccaactatta atcaatacat gccaggtttt actatggata ctaaaaagat attcaagcac
      241 ataccctggg tgattctcgg aatcatcggt gcattctgcc tcgcggtagt tgcattacgt
      301 cggggggagc acgtcagcgc cctgtggatc gtggtcgcct ctgtatcggt gtatctggtg
      361 gcgtatcgct actacagtct gtacatcgcc cagaaggtga tgaaactcga ccccacgcgc
      421 gcgacgcctg cggttattaa caacgacggt ctgaactacg ttccgaccaa ccgttacgtg
      481 ttgtttggtc accacttcgc cgctatcgcc ggtgctggtc cgctggtggg tccggttctc
      541 gccgcgcaga tgggctacct gcctggcacg ctgtggctgc tggcgggggt cgtgctggcc
      601 ggtgcggttc aggactttat ggtgctgttt atctcctctc gccgtaatgg cgcatctctt
      661 ggtgagatga tcaaagaaga gatgggacca gtaccgggga ctatcgcgct gtttggctgt
      721 ttcttaatca tgatcatcat cctcgccgtc ctggcgctga ttgtggttaa agccctggcc
      781 gaaagtccgt ggggtgtctt caccgtttgc tcaaccgtac cgattgcgct gtttatgggt
      841 atctacatgc gctttattcg tccggggcgt gtgggtgaag tctctgtcat tggtatcgtg
      901 ctgctggttg cctctatcta cttcggtggc gtgattgctc acgatccgta ctggggtccg
      961 gcactgacct ttaaagacac caccattacc ttcgcgctga ttggctatgc gtttgtttcc
     1021 gcactgctgc cggtgtggct gatcctcgca ccgcgcgact atctggcaac cttcctgaaa
     1081 atcggcgtta tcgtcggcct ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg
     1141 cctgccatga cccagtacat tgacggtact ggcccgctgt ggaaaggcgc tctgttcccg
     1201 ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct tccacgcgct gatctcttcc
     1261 ggtacgacgc caaaactgct ggctaatgaa accgacgcgc gtttcatcgg ctacggcgca
     1321 atgctgatgg agtccttcgt ggcgattatg gcgctggttg ctgcgtccat catcgaaccg
     1381 ggtctttact tcgcgatgaa caccccgcct gctggccttg gcatcaccat gcctaacctg
     1441 catgaaatgg gtggcgagaa cgcgccgatc atcatggcgc agctgaaaga cgttaccgca
     1501 cacgcggcag cgaccgtcag ctcctggggc ttcgtgattt cgccagagca gatcctgcaa
     1561 accgcgaaag acattggtga gccttctgtc ctgaaccgtg caggtggtgc gccgacgctg
     1621 gcggtaggta tcgctcacgt gttccacaaa gtgctgccga tggctgacat gggcttctgg
     1681 tatcacttcg gtattctgtt cgaagcactg ttcatcctga ccgcgctgga tgcgggtacc
     1741 cgttctggcc gctttatgct gcaagacctg ctgggtaact tcatcccgtt cctgaaaaaa
     1801 accgactctc tggttgccgg tatcatcggt actgcgggct gtgtgggtct gtggggctac
     1861 ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta agagcctgtg gccgctgttc
     1921 ggtatttcca accagatgct ggcagccgta gcgctggtac tgggcaccgt tgtgctgatt
     1981 aagatgaagc gcacccaata catctgggta actgttgttc cggctgtatg gctgcttatc
     2041 tgcaccacct gggcgctggg cctgaaactg ttcagcacca acccgcagat ggaaggcttc
     2101 ttctacatgg caagccagta caaagagaag attgctaacg gtactgacct gacggcgcag
     2161 cagattgcca acatgaacca catcgttgtg aacaactaca ccaacgcggg cctgagtatt
     2221 ctgttcctga ttgtggtgta cagcatcatc ttctacggtt tcaaaacgtg gcttgcggtg
     2281 cgtaacagcg acaaacgtac tgacaaagaa acaccgtacg ttccaatccc ggaaggcggc
     2341 gtgaagatct cttcgcacca ctaaccgtgt ttagccccgc ttcggcgggg ctttgttctt
     2401 atcagagtga actatgtttg gtaacttagg acaggcaaaa aaatatctcg gccaggcggc
     2461 gaagatgttg attggcattc cagactacga caactacgtc gagcatatga agaccaacca
     2521 tcccgacaag ccgtacatga gctatgaaga attcttccgc gaacgccaga atgcgcgcta
     2581 cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg agagagcatg aacccgattg
     2641 cagttaccct actcaccggt tttttaggcg caggcaaaac caccctgctg cgccatattc
     2701 ttaacgaaca acacggctac aagattgccg tgattgaaaa cgaattcggc gaagtctccg
     2761 ttgatgatca actgattggc gaccgcgcca cgcagatcaa aacgctgacc aacggctgca
     2821 tctgctgttc gcgctccaac gagctggaag acgcactact cgacctgctg gataatctcg
     2881 acaagggcaa tattcagttc gaccgtctgg tcattgaatg caccggcatg gccgatcccg
     2941 gcccgattat tcagaccttt ttctcccatg agattttatg ccagcgttac ctgctggacg
     3001 gcgtgattgc gctggtggat gcggtacatg ccgatgagca gatgaaccag ttcaccatcg
     3061 cccagtcaca agtgggctac gccgaccgta ttctgctgac taaaaccgac gtcgcaggcg
     3121 aagcagaaaa actgcgtgaa cgcctggcgc gcatcaacgc ccgtgcaccg gtctacaccg
     3181 tcacccacgg cgacatcgac ctgggcctgc tgttcaacac caacggtttt atgctggaag
     3241 agaacgtcat cagcaccaaa ccgcgtttcc actttatcgc ggataaacaa aacgatattt
     3301 cgtcgattgt ggtggaactg gattacccgg tagatatcag cgaagtttcc cgggtgatgg
     3361 aaaacctgct gctggaatcg gcggataaac tgctgcgtta caaagggatg ctgtggattg
     3421 acggcgaacc taaccgcctg ctgttccagg gcgtccagcg cctctacagc gccgactggg
     3481 accgcccgtg gggcgatgaa aaaccgcata gcacgatggt gtttatcggg attcagttgc
     3541 cggaggaaga gattcgggcg gcgtttgcgg ggttgaggaa gtaattatgg atgatggccg
     3601 ccatgtggcg gcctgaatta ttttttgttc tgatcgcgca gcatcgcttc acgtaaaatt
     3661 gcgttcaggc gagtttgata gcctttacca ggtcgcttca gccattccat cacatcagca
     3721 tcaatacgca ccgaagcctg ggttttcaat ggccgataaa acttaccgcg ttctgcgtta
     3781 gaccattgct catcgctgga aggcgggata tcactgtaat caatatcttc gtcgaattta
     3841 ttcgccagtg ccttcagttc ggcctcatgc tgcacatcga gtgtagaggg tgtccctcgt
     3901 ttatgtttaa ccatgctcat agcggctcct ctctttacga tctgcttttc gtgcactgat
     3961 gattcgaata atttcatttc cgctttcgaa acggatggta tgcgcaacca gaataaccac
     4021 gataccgtga accaggccga tagtctgcca gcgatactca ccgttttcta tgcgatcttg
     4081 ctgtgacaaa tgttgtggat catcaaacac taacaccgca tcttcaaagc ggatgccatg
     4141 tttcacccga ttacttttgg ccttgtttgc atcccattca aactccattg gcatagttgc
     4201 catccctaat tgtatataca aaaatgtatc tacaattatt ctgcaattat agcgttgatg
     4261 ttggaaatag catcacgttt tggataatca accatgaaat tgacaatgag taagttggac
     4321 ttgattcatc caatagaatt tctcgcctga catggcacac taaacacctc gctgtcgtta
     4381 agggattacc gtgaaaaact ataaagttat taccccgctt tttcctacct atgcacaggt
     4441 taaagctatg atgaaagccg tctcaggcta ttccctgaaa gcggtacgca atatgattac
     4501 cgccattcgc gatcaaaccg gtacgccgca aaaacccgtt gactggtcgg aacctgacct
     4561 gtggatcagc gaacgcttaa cgggcgaaga tgccgacatc gcccgtcgta tttgggatac
     4621 agataatcac atattgaacc cgcgccacag ctacggctgc tacttattcc tcaactatcc
     4681 ccagtttgag ctgatggaaa gcacgccaga tgatacctgg cagcccacct cgcgcgggca
     4741 aaaattcctg caagatgacg aaaaaacgct gcgttcactg gatgaccagg aaggcattct
     4801 gcaactgctt gagctgctgg ctggccgaga aatgtcgcgc cgcgccgatt tattgccgga
     4861 atggcaggcc tttttgcatc agcattcgaa attcgcgtct gcaagttcag tgaaaagcac
     4921 gctctactcc cgtctctata accttattga ccgtggcatg gttactcgcg aaggtatgag
     4981 ctattgcatc accgataccg gacgcgcctg gcttggaaaa gcgttgccag cacgaaaaag
     5041 cgatccgcgt aaagagctgc tggaagcggt gaaacgctac aacgagcagc aaaaagagct
     5101 gctgcgcgaa caaatcagca caatgaaccc gtacaaattt gaacaactgg tcggtcaatt
     5161 actggaagca atgggctacg agcaggtcga agtgaccaaa gcctcaggcg ataaaggtgt
     5221 ggacgtgatt ggtcaggtgc aggtcggcat caccactatt accgaagtgg tgcaggtcaa
     5281 aagaatgcag aacaccatca ctcgaccgta tatcgaccag ttgcgcggag cgttgcctta
     5341 ccacaaggcc attcgcggta cgctcatcac caccgggaaa tttgcagcga aatgtgcaga
     5401 agcggcgctc tttccggggg ccgcgcccat tacgcttatc gacggcgatc gcttactgga
     5461 actgttaatc gaaaataacg tcggcattcg ccgcagcaat gcagtggaat tgctggatgt
     5521 ggatttgcag ttgtttgatg agctggagat ggattaaagg gcgaaatccg ccgcctcaac
     5581 cattacaata gcgacccaat ttatctgaac agagttcatc atggccaaag caccaactaa
     5641 aaaagccaaa gcaaaaaaag gctttgaaga aacattatgg gataccgcaa accaacttcg
     5701 cggtagcgtt gagtcctccg aatacaagca cgtggtgtta agcctcgtgt tcctgaaatt
     5761 catcagcgat aagtttgaag cacgccgcaa aaaaatgatt gccgatgggc aagctgactt
     5821 ccttgagatg gaagtgttct accagcagga caacattttc tacctgccgg aagaggcgcg
     5881 ctggtcgttt atcaaacaaa atgccaaaca ggacgatatt gcggttcgta ttgacaccgc
     5941 cctctcgaca attgagaaac gtaacccaac cctgaaaggc gcgctgccag acaactactt
     6001 cagccgtcag aatctggaaa ccaaaaaact ggcgtcactg attgatacca tcgacaacat
     6061 cgaaacgctg gcacacgaaa ctgacgttga aacgttgtcg aaagaagacc tggtcggacg
     6121 cgtttacgaa tacttcctcg gtaagtttgc cgcaaccgaa ggcaaaggcg gtggagagtt
     6181 ctacacgccc aaatgtgtgg tcacgctgtt aactgaaatg ctcgaaccct tccagggcaa
     6241 aatttatgac ccgtgctgcg gctcggcagg gatgttcgtg cagtcggtga agttcgtcga
     6301 gagccatcag ggtaaaagcc gtgatatcgc gctgtatggt caggaactga cagccacgac
     6361 gtataaactg gcaaaaatga acctcgccat tcgcggtctt tcagctaacc tcggcgaacg
     6421 cccggcaaac actttcttta gcgaccagca cccggacctg aaagccgact acattctggc
     6481 gaacccgccg ttcaacctga aagactggcg caatgaagcc gaattaaccg aagatccacg
     6541 ctttgccggt tatcgtatgc cgccaaccgg taacgccaac tacggctgga ttttgcatat
     6601 gctctccaag ctgtcggcta acggcacagc aggttttgtg ctggcaaacg gttcgatgag
     6661 ttctaacacc agcggtgaag gcgagatccg tgcacagatg atcgaaaatg atctgattga
     6721 ctgcatgatc gccctacctg gacagttgtt ctacaccacg cagatcccgg tgtgtttatg
     6781 gtttatgacc aaatcgaagg ctgccgaccc ggccaaaggt tatcgtgatc gtcagggcga
     6841 gacgctgttt attgatgcgc gtaacctcgg caccatgatt agccgcacaa ctaaagagtt
     6901 aacggcggaa gatattgcca caatcgccga tacctaccat gcctggcgca gcacgccgga
     6961 agaactggct gcacggattg cgcgtggtga cagcaagctg gaaaaatatg aagaccaggc
     7021 aggcttctgc aaagttgcga ccctgcaaga tattaaagat aacgactacg ttctgacacc
     7081 gggccgctat gtgggtgcag ccgagcagga agaagacggc gtggcatttg agaccaaaat
     7141 gcgtgaattg tcgaagacgt tgtttgagca gatgaagcag gcggaagaac tggatcgtgc
     7201 gattcgccag aatctggagg cgctgggtta tggggagtaa gtggtgtata tatcgcttag
     7261 gtgatatagc tgaattttca tacggaaaaa tgcctaaaaa ggagtttgtt ggtacaggtg
     7321 actatgtcat tttttcagga tataaataca cagaaagcta ccctgaaaaa aatacttcgg
     7381 caggtgagtt aattgttgtt gcccgaggtg ttggtggaac tggcg
//
LOCUS       DAPQZD010000208         7376 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPQZD010000208 DAPQZD010000000
VERSION     DAPQZD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.210"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(132..257)
                     /locus_tag="RO454_004449"
                     /pseudo
     CDS             complement(132..257)
                     /locus_tag="RO454_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            299..529
                     /locus_tag="RO454_004450"
                     /pseudo
     CDS             299..529
                     /locus_tag="RO454_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            581..1516
                     /gene="ttcA"
                     /locus_tag="RO454_004451"
     CDS             581..1516
                     /gene="ttcA"
                     /locus_tag="RO454_004451"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDY0936179.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1644..3017)
                     /gene="dbpA"
                     /locus_tag="RO454_004452"
     CDS             complement(1644..3017)
                     /gene="dbpA"
                     /locus_tag="RO454_004452"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDY0936180.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3495..4478)
                     /gene="zntB"
                     /locus_tag="RO454_004453"
     CDS             complement(3495..4478)
                     /gene="zntB"
                     /locus_tag="RO454_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDY0936181.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4733..5965
                     /gene="dgcM"
                     /locus_tag="RO454_004454"
     CDS             4733..5965
                     /gene="dgcM"
                     /locus_tag="RO454_004454"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDY0936182.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5986..6549)
                     /gene="smrA"
                     /locus_tag="RO454_004455"
     CDS             complement(5986..6549)
                     /gene="smrA"
                     /locus_tag="RO454_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDY0936183.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6744..>7376)
                     /locus_tag="RO454_004456"
     CDS             complement(6744..>7376)
                     /locus_tag="RO454_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936184.1"
                     /translation="GLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGG
                     FVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVF
                     NLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRGGSD
                     QTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         1985 a         1881 c         1701 g         1809 t
ORIGIN      
        1 atataatcac caaccatacc catcatttat tacagagaat tattttattc accgaaaggt
       61 ttcacaaaat atcccctgat agaaaactaa aaaacactgc ataatatcgc tattctggca
      121 cttgcgaaaa attatattct gcacctgttg tagcgttcta tttctctgcc tggcaacgaa
      181 tgcacaatgt ccgccacagt ttctgttcta atttcttcct gtgttaccgc atcctgtacg
      241 ggtagcgcaa caccagcatg ctaagcaagg ctttcccatc agatttcact acgactattt
      301 gggagcgaga tggaatgcag cagtaaaacg tgctggtatt cgccgccgta atccgtacca
      361 tacgcgacat acttttgcct gctggctgtt gacggcagga gcgaacccgg catttatagc
      421 cagccagatg gggcatgaaa ctgcgcagat ggtgtatgaa atttacggta tgtggattga
      481 tgacatgaac gacgaacagg tagccatgtt gaatgcgcgg ttatcgtagt tgcaaagttt
      541 gccctcaata tgccccattt agtaccagag aactgaaata atgcaagaaa atcaacaaat
      601 tacaaagaaa gaacaataca acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg
      661 cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg tttgcctctc
      721 cgggggtaaa gacagctata ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc
      781 aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccgggct tcccggaaca
      841 cgttctgccc gagtatcttg aaacgctggg cgttgagtac aagattgttg aagagaacac
      901 ttacggtatc gtgaaagaga agattccgga gggcaaaacc acttgctcac tgtgttctcg
      961 tcttcgtcgc ggtatccttt atcgcaccgc aacggaactg ggggcgacga agatcgcgct
     1021 gggtcaccat cgtgacgata tcctgcaaac gctgttctta aatatgttct acggtggcaa
     1081 gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatatcg tgattcgtcc
     1141 gctggcctac tgccgcgaga aagatattca gcgctttgcc gatgcaaaag cgttcccgat
     1201 tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtaa ttgctgacat
     1261 gttgcgtgac tgggataaac gttatccggg gcgtatcgag acgatgttca gcgcaatgca
     1321 gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc gatttcaaag gcattaccca
     1381 cggttctgaa gtggttaacg ggggggatct ggcgtttgat cgcgaagaga tcccactaca
     1441 accagcgggc tggcagccag aagaagatga aaatcagttg gatgagttac ggctgaatgt
     1501 ggttgaagtg aaataaccag gatagcgccc gatacgcaag cgtatcgggc tactcttatg
     1561 gaagccgata agacgcggcc agcgtcgcat ccggcaatgc cgaagaagat gtttactctt
     1621 gcaccctgca attcaacatt tcattatttt aataaccgca cccggcacgt ttttccttta
     1681 atcttcccgc cctgtaactg tttccatgct ttatgagcaa cagcctgacg caccgcgaca
     1741 taaacatgcg ccggatgcac ggcaattttg ccaatatctg cgccatcaag cccgatgtct
     1801 cctgtcagcg cacctaatac atcacccggg cgcattttgg cttttttccc gccatcgata
     1861 cacaacgttg ccatttctgc ttccagcggc acaatggaac tattcgctgg cggcgtttgc
     1921 cagttaagtt ttatctgcaa catgtcagaa atgatattgg cccgctgtgc ttcttccgga
     1981 gcacagaaac tgatcgccag accgctattt cctgcacgag ctgtacgacc gatgcgatgt
     2041 acatgaactt cagggtccca cgccagctca aagttcacca ccagctcaag cgattttata
     2101 tccagaccac gcgcagcaac atcagtcgcg accagtacac gggcgctacc gttagcaaaa
     2161 cgtaccaggg tctgatcgcg atcgcgttgc tccagatcgc cgtgtaacga caatgcactt
     2221 tgccctactt cattcagcgc gtcgcagaca gactggcaat cttttttggt attgcaaaac
     2281 accacgcaag aggatggctg atgcaagctt aataaccgtt gcaacagagg aattttgcct
     2341 ttgctggatg tctcataaaa ttgttgttca ataggtggca gagcatctgt tgagtcaatt
     2401 tcaatcgcta aaggatcgcg ctgcactctt ccgctgattg cggcaatggc ttccggccag
     2461 gttgccgaaa acagaagtgt ctgccgagat gctggcgcaa aacggatgac atcatcaatg
     2521 gcgtcactaa atcccatatc cagcatgcgg tcggcctcat ccatcaccag cgtattcaac
     2581 gcatccagtg atacagtgcc tttttgcagg tgatccagca aacggcccgg cgttgccacg
     2641 ataatatgcg gcgcatgttg taacgaatca cgctgcatac cgaacggttg accaccgcac
     2701 aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc acctgccacc
     2761 tgatccgcca gttcacgcgt aggacacagc actaaagcct gggtttgaaa tagcgacgca
     2821 tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc ggttttcgcc
     2881 tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg caccggcgtc
     2941 atggttaaat aacccaaccc attaaggttt gtgagttggg cgggaggcaa aacattcagg
     3001 gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagtcag caggcgcgta
     3061 tcctcgcaga tctacgctca cgatgcgaca atttaatcgg ctcttcatcg ggtggtgggt
     3121 caggcatggg ttgcgggcga gggatcggat cgggcacggg aacaggatcg ccaggaatcg
     3181 gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc ggtcattggg
     3241 tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga ctcatcaaag
     3301 tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt aagacaatat
     3361 ggagcgcaac gcccatcgct tgacgttgca ttcacctgca agagagatat tgccctgaat
     3421 gggtagagag tttattgact tcgctcaaac tttacggcgt ttttgtatac agacagccgg
     3481 gaaaattgct tttgttacaa ccatttacta cgatgcaacc ataaagcaac accaccaata
     3541 agaacaacta acagaataca aaaaattgaa aatccgaatt gccatccacc gccagggatc
     3601 ccaccaaggt tgacgccaaa taaccctgtc agaaacgtac tgggtaaaaa gaccattgcc
     3661 atcaacgaca ttgtataagt acgacgagct aaattttcct gcatcacctg agcgatttca
     3721 tccgccatca cgccagtccg tgctatacag gcgtcgattt cgtcaagacc gcgcccaagg
     3781 cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg caaacgttca
     3841 ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat cacaattaat
     3901 tgtttgcgca gcagagccag gaatccacgc ggtggaattt gctgatcgag aagattatct
     3961 tcgaggtcga taattttatc gtgcagctgc tcgataaatt cgctggaatg atcggtcaac
     4021 gcatcgcaca catcaaccag ccaccccccg caatcggtcg gaccagtgcc ctcttccaga
     4081 tcgctcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac aattaaccgc
     4141 ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt gctgccgttt
     4201 atacagcgca atgtaatcag cgtgccttca ccgagacggc tgactcgggg ccgtgtgctc
     4261 tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc cagccattgg
     4321 gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc atcaatcaca
     4381 tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccatgc aaatactgca
     4441 tccgggacat taacgtccga tcccttaatc gcttccacag tgcctccatc atcaacgcat
     4501 tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc attaccctga
     4561 aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt tccgtataca
     4621 aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc atatgacgtg
     4681 gcttttttgc gatcggatag caacaacaat tgataaaaat aatgggatct caatgattac
     4741 gcacaacttc aataccctgg acttactcac cagtcctgtc tggatcgttt cgccctttga
     4801 ggaacagtta atttatgcca acagcgaggc gcgactgttg atgcaagacc tcacgtttag
     4861 tcagctacga accggtccct attccgtctc ctcacaaaaa gaactgccga aatacctctc
     4921 cgatctgcaa aatcaacacg atattatcga aatcctcacc gttcagcgca atgaagagga
     4981 aacagcatta agctgtcggc ttgttttgcg agagctgaca gaaacagaac cggtgattat
     5041 tttcgaaggt atcgaagcac cggcaacgct gggtttaaaa gccagtcgct cgacaaatta
     5101 tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg actaactctg cgccaatgtt
     5161 gttgattgac ccctcacgag atggccaaat cgttgatgct aacctcgccg ctctcaattt
     5221 ctatggttat aaccatgaaa cgatgtgcca gaaacatacc tgggaaataa atatgctcgg
     5281 gcgtcgcgtc atgcctatca tgcatgaaat ctcgcattta cccggtggtc ataaaccttt
     5341 gaattttgtt cataaactgg cggatggttc gactcgtcat gtgcagacct atgccgggcc
     5401 gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta ctgagcaaaa
     5461 acggctggag gagcagctgg aacatgctgc ccaccatgat gcgatgacag gattactgaa
     5521 tcggcgacag ttttatcaca ttaccgagcc aggccaaatg cagcacctcg ccatcgctca
     5581 ggattacagc ttgttgctca tcgacaccga tcgttttaaa cacattaacg atctctatgg
     5641 gcattctaaa ggtgatgagg tgttatgcgc ccttgcccgc actctcgaaa gttgcgctcg
     5701 caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtcttattgc taccaagaac
     5761 cccactggat accgcgcttt cgctggctga aactatccgc gtaagcgtgg caaaagtgag
     5821 tatttcgggc ttaccacgct ttactgtcag cattggtgtg gcgcatcacg aaggaaatga
     5881 aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgagcga aaaatgatgg
     5941 acgcaaccgc gtgctggcgg cataagccac ggatgcgtct cgtgatcaac gactgcgctt
     6001 agcatggcgc tcccagtttt cttgcttcgc ctgcgctgtt ttacgtagtg cgacgtaaca
     6061 cgccccgctg ccgccatgat gcggtagcgc agtgcaatat gcctgaacat catcaaattc
     6121 ggtcagccag cgcgccacat agctgcggac aatattggca tgcgatttat catcccgtcc
     6181 tttgccatga ataatcagca cgttacgcaa accatccgcc agggcttgtt gaatgaaact
     6241 gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc tcgcctgttg
     6301 cggatattta ccactgcgca gcttatccag caccccatgt tgcaaccctt cccgccgaaa
     6361 ctccagcggc cgacttagcg ggatgatgtc gagaaatcca gtggtgagga aattatcaag
     6421 ttgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc agtggacatc
     6481 tgtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg atttgtcgtc
     6541 aaggttcatg taacctccta tgcaccttca cacgtcgtga ttcagacggg ttaattgttc
     6601 atacttactg catcgttccc ttcacagatt gaaacaatac tctacttcat acctgtgagt
     6661 atctgcgcag gcggagcatt ttatactagc ctgttagcag tatcgaacag tttagcccga
     6721 gtttaattca gcacaggctc cgatcaggat gatttattca tagcaggaac caacaaaaat
     6781 gcgataaata acagaccact gattagcaga aaggtatccg aaaatgccat agtttgcgcc
     6841 tccctcagca tcaattgact caataatttg gttgaagcca gtatttccga tgtctggtca
     6901 cttcctccgc gattaaaaaa caaagcactt ctggaaatga agtcattcac cgtatgggga
     6961 acagaaacca ttttttcacc cattctcgaa aaatgaaaat tagttcgatt attgagaata
     7021 gagccacaca gggcaatacc aatcgcgccc ccaagattac gtgttaaatt aaatacacca
     7081 gaggctaact tcagccgttc ttttggaatt ccccccagcg ttaatgttac gatgggggcc
     7141 atcgcaaact gctgcgaaat ccctctaatt gcctgtggaa acagtaactc ctgccatccc
     7201 cattcgtggg ttataggggt aaaaagatac attgaaaaca caaatccacc cagccccgcc
     7261 atcaacagcc atcgcagatt tattttttta cttaaccaga agtaaaaggg aacagaaaat
     7321 aactggaaaa tacctgttgt gcaaacggca aaacctatct cttcggcatt taatcc
//
LOCUS       DAPQZD010000209         7268 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPQZD010000209 DAPQZD010000000
VERSION     DAPQZD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.211"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            78..317
                     /gene="chaB"
                     /locus_tag="RO454_004457"
     CDS             78..317
                     /gene="chaB"
                     /locus_tag="RO454_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDY0936185.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            466..1161
                     /gene="chaC"
                     /locus_tag="RO454_004458"
     CDS             466..1161
                     /gene="chaC"
                     /locus_tag="RO454_004458"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDY0936186.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1205..1558)
                     /gene="ychN"
                     /locus_tag="RO454_004459"
     CDS             complement(1205..1558)
                     /gene="ychN"
                     /locus_tag="RO454_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDY0936187.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1743..3137
                     /gene="ychO"
                     /locus_tag="RO454_004460"
     CDS             1743..3137
                     /gene="ychO"
                     /locus_tag="RO454_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDY0936188.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3138..3788)
                     /gene="narL"
                     /locus_tag="RO454_004461"
     CDS             complement(3138..3788)
                     /gene="narL"
                     /locus_tag="RO454_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDY0936189.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3781..5577)
                     /gene="narX"
                     /locus_tag="RO454_004462"
     CDS             complement(3781..5577)
                     /gene="narX"
                     /locus_tag="RO454_004462"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDY0936190.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5916..>7268
                     /gene="narK"
                     /locus_tag="RO454_004463"
     CDS             5916..>7268
                     /gene="narK"
                     /locus_tag="RO454_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDY0936191.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         1685 a         1794 c         1927 g         1862 t
ORIGIN      
        1 atgaaaattc ttatgttttg gcaagagtag atattgttga ccacacttaa tgttcaactt
       61 tgtaaaagga gtcaacgatg tcgtataaaa cgaaaagcga tctgccggaa agcgtaaagc
      121 acgtgcttcc gtctcatgcc caggatatct ataaagaagc gttcaacagc gcatgggatc
      181 aatataaaga taaagaagat cggcgtgatg acgccagtcg cgaagaaaca gcgcataaag
      241 tggcctgggc tgctgtgaag catgaatatg ccaaagggga tgatgataaa tggcataaaa
      301 aatcgtcaaa ccggtagctt agttaaagct attcgtgcgg tgttgccttg caagtggtcc
      361 gtggattgca tattgtcccg ttagtggttt caaaatgagc agtaaaaatg tccggaagac
      421 accaaaaagt tgtcgcaggg aagtatgcag tggcggaggt gtaaggtgat aacgcgtgat
      481 ttcttgatga atgccgattg taaaacggca tttggtgcca ttgaagaatc actcttatgg
      541 tcagcagaac aacgggcggc ttcgctggcg gcgacgctgg cttgtcgacc tgatgaggga
      601 ccggtgtgga tcttcggtta tggatcgttg atgtggaatc cggcactgga gtttaccgag
      661 tcgtgcaccg gtacactggt tggatggcat cgcgcgtttt gcctgcgtct gaccgccggg
      721 cgcgggactg cgcaccagcc gggacgaatg cttgcactga aagagggcgg acgcaccaca
      781 ggcgtcgcct atcgactgcc agaagagacg ctggagcagg aactaaccct gctgtggaag
      841 cgtgagatga ttaccggctg ttatctgcca acctggtgtc agcttgatct tgatgatgga
      901 cgcacagtaa acgccattgt gtttattatg gacccgcgac atccagaata tgaatctgac
      961 actcgcgctc aggtcatagc gccgttgatt gccgcggcga gcggtccgct gggaaccaat
     1021 gcacaatacc tgttttcact ggaacaggag cttatcaaac tgggaatgca ggacgatggg
     1081 ctgaatgatt tgctggtatc ggtaaaaaaa ctgctggcgg agaattatcc ggatggtgtg
     1141 ttacggccgg gattcgcctg agtaaacttc cctcatagtg gggcgtccga cgccccctca
     1201 aacattaaaa tgtgagcact ttatcggctg acagcgtcca ttgcgccagt tcaacaagag
     1261 taccgatttc caccccatca atcagaggaa gtgtactaat cccgcgcccg tcggtacagg
     1321 ttttgcacaa tttcaccggt acattctgag cggtaaggat ctccagcatt tgctgaatgt
     1381 tgtagccttc ccctggtttt tgcccgcgca acccggctgt gaccgcatca gacatcagga
     1441 acagacgcag atccagattg ttctcctgct ctcgtaacgc aatggccagc cgcaagctgt
     1501 taaacaagga ttcgctcccg taaggtgcgc cattggcaac gatcacgatt ttttgcatta
     1561 tttactcctg tattcaggga attagacact catcttctat cttactgctt ctgcagcgtc
     1621 tgaccaatcg gtcacatttt taaggatttt cctgaaagcg cgagaaaata cgacaaaagg
     1681 tgccagtaat cgttattctt taaggctatg gtttttcatt tttaccggaa gttaccgacg
     1741 ttttgagccg tttcgttcct cgcattattc cgttttattt actcttgctg gcggcaggcg
     1801 gtacagctaa cgcacaatct accttcgagc aaaaagcggc aaatcacttt gagaatagca
     1861 atgatggtct gccggattta ggcatggcac ctgaacatca tgatggggaa aaacactttg
     1921 ctgaaattgt gaaagatttc ggcgaaacca gtatgaatga taacgggctg gatactggcg
     1981 agcaggcaaa agctttcgca ttgggaaaag tccgcgatgc gcttagtcaa caggttaatc
     2041 agcacgtaga gtcctggcta tcaccgtggg gaaatgccag tgttgacgtc aaagtggata
     2101 acgaaggaca tttcaccggc agtcgtggaa gctggtttgt gccgttacaa gataatgatc
     2161 gttatctcac ctggagccag cttggtctta ctcagcagga tgatgggctg gtgagcaatg
     2221 tgggcgttgg gcaacgctgg gcgcgcggca gctggctggt gggttataac actttttatg
     2281 acaacttgct ggacgaaaat cttcagcgag cgggctttgg cgctgaagcg tggggcgaat
     2341 atttgcgact atcggcaaac ttttatcagc cgtttgctgc atggcatgaa cagactgcca
     2401 cgcaggaaca acggatggcg cgcgggtacg acctgacagc ccggatgcgc atgccgttct
     2461 atcaacacct caataccagt gtcagcgtag aacagtattt tggtgatcgt gtcgatttgt
     2521 ttaactctgg tacgggttat cacaatcccg tcgcgttgag tctgggatta aattacaccc
     2581 ctgtgccctt agtcactgtg acggcccagc ataaacaggg tgaaagtggc gagaatcaaa
     2641 ataacctcgg gctgaatctt aactaccgct ttggtgtacc gctcaaaaaa caactttctg
     2701 cgggcgaggt tgtcgaaagt cagtcgttac gtggtagtcg ctatgacaat ccgcagcgaa
     2761 ataatcttcc gactcttgag taccgacagc gaaaaacgtt aacggtgttt ctggcgacac
     2821 cgccgtggga tctaaaacct ggcgaaacag tgccgctgaa attacaaatc cgcagtcgtt
     2881 acggtattcg gcaactgatt tggcagggcg atacgcagat attaagtttg acgccgggcg
     2941 cacaagccaa cagtgaggag ggctggacgc tgatcatgcc tgactggcag aacggggaag
     3001 gggcgagcaa tcactggcga ttgtctgtag tggtggaaga taaccagggg cagcgtgtct
     3061 cctccaatga gatcacgcta acgcttgtcg aaccgttcga cgcattgtca aacgacgaac
     3121 tgcgctggga accgtaatca gaaaatgcgc tcctgatgca cccataccgc agcttccacg
     3181 cgagacttta gcttcatttt cttcagcatg tgcttgacgt gaacttttac tgtgctttcg
     3241 gtgatgtcca ggcggcgggc aatcatcttg ttcggcaaac cctgggcaat cagcttgaga
     3301 atatcgcgct cgcgtggggt taactggtta acatcgcgct cagtagtggc acggttagcg
     3361 cgcaagctgg cggccagaac aggcgttaat gcttcgctta ataccatttc gccagcagct
     3421 gcctgatgta acgctttcag cagatcttcc ggttccatat cttttaacag atagccatcc
     3481 gcgccgcgtt tcagtgcggt gaccacatct tcttcatggt tagagacgct gaataccaca
     3541 atgcgcccgg agagggactt ttcgcgcagt ttatccagcg tttccagacc gttcatgccg
     3601 ggcatattga gatctaacag gatcagatcg ggatcaagag actccgccag ttcaataccc
     3661 tgttcgccat tactcgcttc gccaaccaca gtgatatctg gtgccatact gataagctgt
     3721 tttacgccag ttcgcaacat cgggtgatcg tcgatcagca ggatagtagc cggttcctga
     3781 ttactcatgg gtatctcctt ggacgtctgt gaaagttttt tcgggaataa aggtgaccac
     3841 cacttcggtg ccacctgatt cacgacggcg gacgcggcaa tcgcctcgta aactttgcgc
     3901 acggtcgcgc attattatca tgccgtagtg attgctgcgg atggcatttt caggcacgcc
     3961 gcagccgtta tcctggacgg tcagtttgac ctgattatcg ttttgcgcca ccgtcaccac
     4021 gacctcactc gcttgcgaat gtttgagggc gttacttaat gcctcacggg caatttgcaa
     4081 caagtggatt gcctgatgcg aaggcaccag acgaggcggc aattgataat ccagcttcac
     4141 cgggaagcca aatttggcgc tgtactcttc gcaactcgcc tccagcgccg gacgtaatcc
     4201 aggctcggtg agctgcaaac ggaatgtggt gagcaattca cgcaactgca cccaggatgc
     4261 attcagttcg ttacggatct gacttaacag ttcgcggctg ctttctggca gcgcatcgcc
     4321 ctgcatctgt aagcaactca cctgcatctt catgcaagag agagattggg caatagaatc
     4381 atgcagttcg cgcgcaatgg tggcacgctc ttccatcacg atcaactgtt gctgacgttc
     4441 ctgatggcga tccagcgcca gcgtggcggt gagttgttca accagggtat ccaccagttg
     4501 ttgttgatca tggctaagat gacgcccctg cggcagggtc gccagcaaaa taccgtactg
     4561 cgtatgagtg tcagccagcc gccacttcag ggttgtgccg cgatcgccaa cgggtaatac
     4621 gccgcgcggg cagagctggc agcctttatc atcacaagtc atatctgact ggcaggtaaa
     4681 ctcctgatga ttctcttcat catcagtgtc atacacccgc agttcgatat cacgtagcag
     4741 ggttaaattc tgtaagccgt tgagtacagg tgacaggcgt tcacacagcg gggcgcggga
     4801 atgcaaacgg cggttagcct gccataaaaa agagaggatc tgatttttat gctccagccc
     4861 agcggttttc tcctgaaccc gctgctcaag tacggcataa ctttccgcca gttctgcaga
     4921 catattgttc aacgcagttc caagcatcgc catttcgttg cgcccgctga tgtttgcgcg
     4981 ttgggtaaaa tcgcgatggc tgacggcact cgccattgcc agcagttgcc gccacggttg
     5041 tagcaatctc gcccgcaacc agataatagt gaataccagt aaaagtgcca taaataccgc
     5101 cattacccga tggaccagca ccactgtctc aatgcgcatt tccgtggtgc gatcaaaacc
     5161 agataccagc tgatcaagcc cggcaacaaa ctggctgaca tccgctgaca ccgtttcgcg
     5221 gttttgtgca cgtatcagtg cagggatcag ttcattacgc cagtaatctt gtaaaccctg
     5281 taattgcgcc agttgcccgt ctcgttctgc tgctcgagtc aactcggcgc taaatgctgt
     5341 ttgttccatc tctttaatta agggcttgtc tttctcgctt aatggcactg ctgccaacag
     5401 acggtaactt tgcatgcgca gcgatcccgc tttgttgatc gcatgggcgc tgccctgaac
     5461 gccttgcacc agccagccag aaaccgccat ccctgccagt ccaatagcag tagaaagcaa
     5521 cacaataagc gcaacctgat taaccagggt gagcggagag agacaacgtt taagcatgta
     5581 aacctcttcc ttcaggcttt aaatgagcaa taaccttaat gaatgtgacg atacattctg
     5641 gaatggcagt attctcggct attggctgaa gtatacccat acccggaaag agttactcct
     5701 tatttgccgt gtggttagtc gctttacatc ggtaagggta gggattttac agtaccgtga
     5761 aaaatctcat aatttttatg aaatcactgt actcactatg gataatgata aatatcaatg
     5821 atagataaag ttatcttatc gtttgattta catcaaattg cctttagcta cagacactaa
     5881 ggtggcagac atcgaaacga gtatcagagg tgtctatgag tcactcatcc gcccccgaaa
     5941 gggctactgg agctgtcatt acagattggc gaccggaaga tcctgcgttc tggcaacaac
     6001 gcggtcaacg tattgccagc cgcaacctgt ggatttccgt tccctgtctg ttgctggcgt
     6061 tttgcgtatg gatgttgttc agcgctgttg cggtgaactt accgaaagtc ggttttaatt
     6121 ttacgaccga tcagctattt atgttgactg cgctgccttc ggtttctggc gcgttattac
     6181 gtgttccata ctcctttatg gttcctatct ttggtggtcg tcgctggacg gcgttcagca
     6241 ccggtattct gattattcct tgcgtctggc tgggttttgc cgtgcaggat acctccacgc
     6301 cttatagcgt cttcatcatc atttctctgc tgtgcggctt tgctggcgcg aacttcgcat
     6361 ccagtatggc aaacatcagc ttcttctttc cgaaacagaa gcagggtggc gcgctgggtc
     6421 tgaatggtgg tctgggcaac atgggcgtca gcgtcatgca gttggttgct ccgctggtgg
     6481 tatcactgtc gattttcgca gtatttggta gccagggcgt caaacagccg gatgggactg
     6541 agctgtatct ggcgaatgcg tcctggatat gggtgccgtt ccttgccatc ttcaccattg
     6601 cggcgtggtt tggcatgaac gatcttgcta cctcgaaagc ctccatcaag gagcagttgc
     6661 cggtactcaa acggggtcat ctgtggatta tgagccttct gtatctggca accttcggct
     6721 ccttcatcgg cttctccgcg ggctttgcga tgctgtcaaa aacgcagttc ccggatgttc
     6781 agattctgca atacgctttc ttcgggccgt ttattggtgc gctggcgcgt tctgcaggtg
     6841 gtgcattatc tgaccgtctg ggcggaactc gtgtcacgct ggtgaacttt atcctgatgg
     6901 cgattttcag cggcctgctg ttcctgacct taccgactga cggacagggc ggaagcttca
     6961 tggcgttctt cgcagtcttc cttgcgctgt tcctgaccgc tgggctgggt agtggttcca
     7021 ctttccagat gatttccgtg atcttccgta aactgacaat ggatcgcgtg aaagcagaag
     7081 ggggttctga cgaacgtgcg atgcgtgaag cggcaaccga cacggcggcg gcgctgggtt
     7141 tcatctctgc gattggcgcg attggtggct tctttatccc gaaagcgttt ggtagctcgc
     7201 tggcattaac gggttcgcca gtcggcgcaa tgaaagtatt tttgattttc tatatcgcct
     7261 gcgtggtg
//
LOCUS       DAPQZD010000210         7263 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPQZD010000210 DAPQZD010000000
VERSION     DAPQZD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.212"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            108..1328
                     /gene="arcA"
                     /locus_tag="RO454_004464"
     CDS             108..1328
                     /gene="arcA"
                     /locus_tag="RO454_004464"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDY0936192.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRDQGVEVLLLTDLLTQTLDIKEAKTWLLETQISDYRLGPTFAGD
                     VRSWLADMPHRELARRLSGGLTYGEIPAAINNMVVDTHTSNDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPAFADGDFIKYFGDENIYYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLANSLFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDVDTFSVYPEVVRKDAQCWTLTSNGRDGLQRTQETDLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTIRPGVVIGYERNVWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLERDGI"
     gene            1339..2250
                     /gene="arcC"
                     /locus_tag="RO454_004465"
     CDS             1339..2250
                     /gene="arcC"
                     /locus_tag="RO454_004465"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDY0936193.1"
                     /translation="MERKPTLVVALGGNALLKRGEPLEAEIQRQNIELAARTIAGLTV
                     NWRVVLVHGNGPQIGLLALQNSAYDKVTPYPLDVLGAESQGMIGYMLQQALKNSLPQR
                     EVSVLLTQVEVDATDPAFSNPTKYIGPVYNEDQAKTLAAEKGWGFKADGSYFRRVVPS
                     PQPKRIVESDAITALIQRDHLVICNGGGGVPVVEKANGYRGIEAVIDKDLSAALLAYQ
                     IGADALLILTDADAVYLDWGKPTQRPLAQVTPELLRGMQFDTGSMGPKVAACCKFVEA
                     CNGIAGIGALVDGAEILAGNKGTLIRN"
     gene            2335..3339
                     /gene="argF"
                     /locus_tag="RO454_004466"
     CDS             2335..3339
                     /gene="argF"
                     /locus_tag="RO454_004466"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDY0936194.1"
                     /translation="MTINLKKRNFLKLLDYTPAEIQYLIDLAIKLKAAKKAGREKQTL
                     VGKNIALIFEKTSTRTRCAFEVAAFDQGAQVTYLGPGGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQAIVEELGKYAGVPVWNGLTDEFHPTQILADLMTMLEHSPGKKLSELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDVVLVEQCRSIAEETGARITLTD
                     DVEEGVWGTDFLYTDVWVSMGEPKEAWTERVSLMKPYQINADVMNATGNPNVKFMHCL
                     PAFHNEHTKVGREIEMAYGLKGLEVTEEVFESPNSIVFDEAENRMHTIKAVMVATLDD
                     "
     gene            3387..4790
                     /locus_tag="RO454_004467"
     CDS             3387..4790
                     /locus_tag="RO454_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDY0936195.1"
                     /translation="MGKFKFPSAYTILFFLIAIVAVLTWIIPAGQYHMAMNEALGKEV
                     PVAGTYAHVEANPQGLISVLMAPIAGLYDPDSGQARAIDVALFILIIGGFLGIVTKTG
                     AIDAGIERVTTRLRGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMLAARFD
                     PVVAASTVLLGAGIGTLGSTINPFATVIAANAAGIPFTNGITLRVVVLVIGWIICVTW
                     VMRYARKVRKEPSLSIIADKQEENLAHFLGNKSEQALEFTPVRKIILVIFALTFAVMI
                     YGVAVLGWWMAEISTVFLASAIIIGLIARMSEEELTSTFINGARDLLGVALIIGIARG
                     IVVIMDKGMITHTILHYAEGMVTGLSTVAFINVMYWLEVVLSFLVPSSSGLAVLTMPI
                     MAPLADFANVNRDLVVTAYQSASGIVNLITPTSAVVMGGLAIAHVPYVRYLKWVAPLL
                     GILTVVIMVALSLGALL"
     gene            4871..5350
                     /locus_tag="RO454_004468"
     CDS             4871..5350
                     /locus_tag="RO454_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDY0936196.1"
                     /translation="MMDYEDFSPKEQLQLTVCQRLIAEKSYFSQEELRRDLQERGFET
                     ISQSTVSRLLNLLGVIKIRNAKGLKVYSLNPQLRPAPDAARTVSEMVVSVEHNREFIL
                     IHTVAGYGRAVARVLDYHQLPEILGVVAGSSIVWVAPRMVKRVALVHKQINYLLRTY"
     gene            complement(5414..5755)
                     /locus_tag="RO454_004469"
                     /pseudo
     CDS             complement(5414..5755)
                     /locus_tag="RO454_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5707..5928)
                     /locus_tag="RO454_004470"
     CDS             complement(5707..5928)
                     /locus_tag="RO454_004470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936197.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQARCRKNPGWAKRSAMY"
     gene            complement(5959..6309)
                     /gene="tnpB"
                     /locus_tag="RO454_004471"
     CDS             complement(5959..6309)
                     /gene="tnpB"
                     /locus_tag="RO454_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDY0936198.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPVVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(6306..6668)
                     /locus_tag="RO454_004472"
     CDS             complement(6306..6668)
                     /locus_tag="RO454_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDY0936199.1"
                     /translation="MRMVELASQPGACVAQIARENGVNDNVIFKWLRLWQNEGRISRR
                     IPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTTVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            complement(6777..7263)
                     /locus_tag="RO454_004473"
                     /pseudo
     CDS             complement(6777..7263)
                     /locus_tag="RO454_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1709 a         1912 c         1936 g         1706 t
ORIGIN      
        1 tttaccgcct agtatgaacc acgaaatcaa ttgaagttag ccgccatgaa attaaataca
       61 caacatggcg catacctttt tattctaaaa aaattaaagg aataattatg gaaaaacatt
      121 acgtcggttc tgaaattggt caattgcgta gtgttatgct gcaccgccca aatttaagtc
      181 tgaaacgttt gacaccatcg aattgtcagg aactgctttt cgatgatgta ctctcggttg
      241 aacgggcagg tgaagagcat gacatcttcg caaatacgct gcgcgatcag ggggtggaag
      301 tcctgctgtt aacagacctc ctgacacaaa cccttgatat taaagaagcg aaaacttggt
      361 tactggagac gcaaatttct gactaccgcc tcggacctac ctttgcgggc gatgtgcgca
      421 gctggctggc ggacatgccg caccgtgaac tggcgcgaag attaagcggc ggattaactt
      481 acggtgaaat tccggctgcc attaacaata tggtggtgga tacccacacg tctaatgact
      541 ttattatgaa gccgctaccg aatcatttat ttacccgcga tacctcctgc tggatttata
      601 acggtgtttc tattaacccg atggccaaac cagcccgtca acgtgaaacc aataacctcc
      661 gggcaatata tcgctggcac ccggcatttg ccgacggcga ttttattaag tatttcggcg
      721 acgaaaatat ttattacgac cacgccactt tggaaggtgg cgacgtatta gtgattggtc
      781 gtggggcggt attgatcggc atgtctgaac gcacaacacc gcagggcgtg gagttcctcg
      841 ccaacagcct gttcaaacat cgtcaggccg agcgagtgat cgccgttgag ctgccaaaac
      901 accgctcctg tatgcacctt gacaccgtca tgacccacat cgacgttgac actttctccg
      961 tttacccgga agtggtgcgc aaagacgccc agtgctggac gctcacttcg aacggacgcg
     1021 atggcctaca acggacccag gaaaccgacc tgttgcacgc catcgagaaa gcactcggta
     1081 ttgaccaggt acgcttgatc accaccggcg gcgacgcctt tgaagccgaa cgtgagcagt
     1141 ggaacgacgc caataacgtt ctgaccatcc gccccggtgt ggtgattggt tacgagcgca
     1201 acgtctggac taacgagaaa tacgacaaag ccggcatcac cgtgctgccc atcccggggg
     1261 acgaattggg acgaggccgc ggcggcgcac gctgcatgag ctgtccgctt gaacgcgacg
     1321 gaatttaaag gagccatcat ggaacgaaaa cccactttgg ttgtggcgtt gggcggcaac
     1381 gcattattga agcgcggcga accactggaa gcagaaatcc agcgccagaa cattgagttg
     1441 gccgcccgta ccatcgccgg gctcacggtg aattggcgcg tggtgttggt tcacggcaac
     1501 ggtccacaga tcgggctgct ggcgctgcag aacagcgcct acgacaaagt gaccccttat
     1561 ccactggacg ttcttggcgc cgaaagccag gggatgatcg gctacatgct ccagcaggcg
     1621 ctgaaaaaca gcctgccaca gcgtgaggtg agcgtcctgc ttactcaggt ggaagtggac
     1681 gctactgacc cggcgttcag caacccgacc aaatatatcg gaccggtgta caacgaagac
     1741 caggcaaaaa cactggcagc agaaaaaggt tgggggttta aggccgacgg cagctacttc
     1801 cgtcgcgtgg tgccatctcc acagccgaaa cgcattgtcg agagcgatgc tattacggca
     1861 ctgatccagc gcgaccatct tgttatctgc aacggcggcg gtggtgtacc agttgtggaa
     1921 aaggctaacg gctatcgcgg aattgaggcg gtgatcgaca aagacctctc tgctgccctg
     1981 ctggcatacc agataggggc cgacgcacta ctgattctca ctgatgccga cgcggtttac
     2041 ctcgattggg gcaaaccgac ccagcgtccg ctagcgcagg tgacgccaga actgctcaga
     2101 ggcatgcagt ttgacaccgg atcgatgggg ccgaaagtgg ccgcctgctg caagtttgtt
     2161 gaagcttgca acggtattgc cgggatcggc gctctggtcg acggggctga gattttggcg
     2221 ggcaataaag gcacattgat tcgtaactga atcccccttc acctaaccct ctcctcaaag
     2281 gggagatggc agagtgaggg catcagacag ttaaaattta aaaaggattt cctaatgacc
     2341 atcaatttga aaaaacgcaa cttccttaaa ctgctggact acaccccggc agagatccag
     2401 tacctgatcg atctcgcgat caaactgaaa gcggccaaaa aagccggacg agaaaaacag
     2461 accttggttg gcaaaaacat tgccctgatt tttgaaaaaa cctccacccg tacccgctgt
     2521 gctttcgaag tggctgcgtt cgaccaaggg gcgcaggtga cctacctcgg cccaggcgga
     2581 tcgcaaatcg gccataaaga gtcaatgaaa gacaccgccc gtgtgctggg ccgtatgtat
     2641 gacggcatcg aataccgtgg ttacggtcag gccatcgttg aggagttggg caaatacgcg
     2701 ggcgtaccgg tgtggaacgg tctgaccgac gaatttcacc caacccaaat cctcgcagat
     2761 ttgatgacca tgctggaaca ttccccgggc aaaaaactgt cggaactgag ctttgcctac
     2821 cttggcgacg cacgcaacaa catgggtaac tccctgatgg tgggggctgc caaaatgggg
     2881 atggatatcc gtcttgtagc cccaaaatcc ttctggccgg atgtggtgct tgttgaacag
     2941 tgccgttcca tcgcggaaga gacgggcgca cgtatcaccc tgaccgatga cgtggaagaa
     3001 ggcgtgtggg gaacggattt cctctacacc gatgtttggg tctcaatggg tgaaccgaaa
     3061 gaggcgtgga ccgaacgcgt cagcctgatg aagccttatc aaatcaacgc tgacgtgatg
     3121 aacgccaccg gcaacccgaa cgtcaagttc atgcactgcc tgccagcctt ccacaatgag
     3181 cacaccaaag tgggccgaga aattgagatg gcatacggcc tgaagggact ggaggtgacg
     3241 gaagaggtct tcgaatcccc taactctatc gtctttgatg aagcagaaaa ccgcatgcat
     3301 accattaaag cggtcatggt ggcgacactc gacgactaat caccacccgg tgcgtcgtag
     3361 ggggcaccgg gtctcaggag aacatcatgg gtaagttcaa atttccctcc gcatatacca
     3421 ttctcttttt tctgattgcc atcgttgccg tcctgacgtg gatcattcca gccgggcagt
     3481 atcacatggc aatgaacgaa gctctcggca aggaggttcc tgttgccggc acctatgcac
     3541 acgtagaggc caatccgcag ggactgattt cagtgctgat ggccccaatt gccgggttgt
     3601 atgatccaga ctccggtcag gctagggcga tagacgttgc gctgtttatt ctgatcatcg
     3661 gaggattcct cgggatcgtc accaaaaccg gggccattga cgccggaatc gagcgcgtca
     3721 ccacccgact acgtggtcgc gaagagtgga tgatcccgat ccttatggcg ctgtttgctg
     3781 ctggcggtac aatttacggc atggccgaag aatccctgcc gttttatacc ctgctggtgc
     3841 cggtgatgtt ggcagcacgc ttcgaccctg tggtagccgc ctccaccgtg ctgctcggcg
     3901 ccgggatcgg cacgctcggc tccaccatta accctttcgc gacggtgatc gccgccaatg
     3961 cagccgggat ccccttcacc aacggtatca ccttgcgtgt ggtggtgctt gtcatcggct
     4021 ggataatctg cgtgacatgg gtgatgcgct atgcccggaa agttcgcaag gagccgtctc
     4081 tctccattat tgcggataaa caagaggaga acctcgccca cttcctcggc aataaaagcg
     4141 aacaggctct ggagttcacc ccggtacgca aaattattct ggtgattttc gcccttacct
     4201 tcgcggtcat gatctacggc gtggcggtgc tgggttggtg gatggcggag atctcaacgg
     4261 tatttctggc cagcgcaatt atcatcggtc tgatcgcccg catgagcgaa gaggaactga
     4321 cctctacctt tatcaacggc gcgcgagatt tgctgggcgt tgcactgatt atcggtatcg
     4381 cgcgcggtat cgtagtgatc atggataaag gtatgattac ccatactatt ttgcactacg
     4441 ccgagggaat ggttactgga ttatcgacag tagcattcat caacgtgatg tattggctgg
     4501 aagtggtgct gtcgtttctt gtgccttctt cgtccggtct ggccgttctg acgatgccga
     4561 tcatggcacc tcttgccgac ttcgctaacg tcaaccgcga cctggtagtt acggcttacc
     4621 agtctgcgtc cggtatcgtt aacctgatca ctcccacctc tgccgttgtg atgggcggac
     4681 tggctatcgc ccacgtgcct tacgtgcgat atctgaaatg ggttgcgccg ctgcttggga
     4741 tattaacagt ggtaattatg gtggcattaa gtctgggggc attgttgtaa tttgccggat
     4801 ggcgctgccc ctatccggtc tacggaatgg tgtggtgtca ccggttattt cgagaactga
     4861 atataggatt atgatggatt acgaagattt ctctcccaaa gagcaactac agctaacggt
     4921 ctgccaacgt ctgattgcag aaaagagcta tttttcccaa gaggagcttc gccgcgactt
     4981 acaggagcgt ggttttgaga caatcagcca gtccaccgtt tctcgtctgc tcaatttgtt
     5041 aggtgtcata aaaattcgaa atgccaaagg gctaaaagtc tattcgctga atccacagtt
     5101 gcgtccggct cctgatgccg cgcgcactgt gtccgaaatg gtggtgagcg ttgagcacaa
     5161 tagagaattt atccttatcc ataccgttgc tggatatggc cgcgcagtgg cacgtgtcct
     5221 tgactatcac cagttaccag aaattttagg cgtggtggcc ggaagcagta tcgtctgggt
     5281 cgctcctcgg atggtgaagc gtgtcgctct ggtgcataag caaattaatt atttactaag
     5341 aacgtattaa tattcacaaa tgcccgcttg tattgactaa tacgtaagcg tcaacggagc
     5401 accgtattga cgcttattta ttggtgagaa ctacgttcca tggcaggagt tcgtcaacac
     5461 ggttggaggg ccattccggc agtacgctca gaatatggcg cagatacgct tccggatcga
     5521 taccgttcag acggcaggtg ccgatcagcc cgtacagcag tgcactacac tcgccgccgt
     5581 gatcgctacc gaagaacacg taatttttct ttccgagaca gaatgcacga agcgctcttt
     5641 ccgctgtgtt attgtccgcc tccgccagac cgtcatcact gtaataacag agggcgtccc
     5701 actgattcag tacatagctg aacgcttcgc ccagcctgga ttttttcgac agcgtgcctg
     5761 agcccgcaga tgttctattt cccgttcatc ttcttcgatc ttttcttcgg cacgtgccag
     5821 tgcagagcgc aggaaggcct ccgtctcttc aaccagactc agttgctggt ctttctgacg
     5881 gagctggctt tccagttctg caatgcgaat gaggtatttc tgactcatgg ccgtttttat
     5941 aatgcggcca ggcgtttttt acaacattgt cagggcgtta aggcgggatg tttttggctg
     6001 acgccagtcc agcttatcga ggagcattgc cagttgcgag cgggtaatgg ataccttgcc
     6061 gtcacgtacc acaggccaga taaactggcc ttcctccagg cgtttggtga acaggcacag
     6121 accatcagca tcagcccaaa gaattttaac ggtgtcaccc cgtcggccac ggaagataaa
     6181 caggtgaccg gagaagggat tatcattcag cacatgttgt acctgttctc ccagtccgtt
     6241 gaaggattta cgcatatcgg taacgccggc aacgagccag atacgggtac ctgatgggag
     6301 tgagatcatc ttcccctccc ggtcagttca cgaatcaata cagtgagcag ctctggtgaa
     6361 ggattttcca gcgtcatgtt accgtgacgg aactccacct tgcaggagct ggcactgaca
     6421 gtagtctgag tggataagga cggagtaaga gcagccatcg gttctttcgg ctcatcaggc
     6481 gttatctcta caggtaataa ttcaacgcct gcgtcagaag tggttgtcac cggaatacgc
     6541 cgtgatatac gcccttcgtt ttgccagagt ctgagccatt tgaaaataac attatcattg
     6601 acgccatttt ctcgtgcaat ctgggccaca caagcaccgg gttgtgatgc cagttcaacc
     6661 atacgaattt tgaattcatt cgaatacttt ttacgaggtt cttttcgtca gtcctgtaat
     6721 tccatactta gatgtccgtc tgtgtcagat gggcgtctaa gggtaatgac tccaacttat
     6781 tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
     6841 gaaaacgaca gcgacttccg tcccagccgt gccaggtgct gccacagatt caggttatgc
     6901 cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
     6961 tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
     7021 ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
     7081 cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
     7141 cactgttcgt ccatttccgc gcagacgatg acgtccccgg ctgtatgcgc gaggttaccg
     7201 actgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc ccataatgcg
     7261 ggc
//
LOCUS       DAPQZD010000211         7254 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPQZD010000211 DAPQZD010000000
VERSION     DAPQZD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.213"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(255..959)
                     /locus_tag="RO454_004474"
     CDS             complement(255..959)
                     /locus_tag="RO454_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDY0936200.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1096..1548)
                     /gene="moaE"
                     /locus_tag="RO454_004475"
     CDS             complement(1096..1548)
                     /gene="moaE"
                     /locus_tag="RO454_004475"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDY0936201.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1550..1795)
                     /gene="moaD"
                     /locus_tag="RO454_004476"
     CDS             complement(1550..1795)
                     /gene="moaD"
                     /locus_tag="RO454_004476"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDY0936202.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1788..2273)
                     /gene="moaC"
                     /locus_tag="RO454_004477"
     CDS             complement(1788..2273)
                     /gene="moaC"
                     /locus_tag="RO454_004477"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDY0936203.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2276..2788)
                     /gene="moaB"
                     /locus_tag="RO454_004478"
     CDS             complement(2276..2788)
                     /gene="moaB"
                     /locus_tag="RO454_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDY0936204.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2810..3799)
                     /gene="moaA"
                     /locus_tag="RO454_004479"
     CDS             complement(2810..3799)
                     /gene="moaA"
                     /locus_tag="RO454_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDY0936205.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3787..3931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4196..5104
                     /gene="yvcK"
                     /locus_tag="RO454_004480"
     CDS             4196..5104
                     /gene="yvcK"
                     /locus_tag="RO454_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDY0936206.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5142..7163)
                     /gene="uvrB"
                     /locus_tag="RO454_004481"
     CDS             complement(5142..7163)
                     /gene="uvrB"
                     /locus_tag="RO454_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDY0936207.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1755 a         1923 c         1818 g         1758 t
ORIGIN      
        1 cttcgtgctg aaaatctaat ttattgctat tttgcgagta tgattccatt cttttagttg
       61 tccttagaaa aaacattaat attcaccatc aataaattta aaaccacgca tacaaaatac
      121 ttttgcattt atggatacag aaaacgcttc cgctcgtcca taaggcatat tttatacctg
      181 gtacttgtta agtgtcgttt ttaataagaa aaaaaatccc gacacatggc cgggatattg
      241 atgatgaaaa agaattaacg gcggttgccg aagatccgca gcaacatcag gaacaggttg
      301 atgaagtcca gatacaaggt taacgcgcca agaatggaat atttgcgcag gttcgacgtg
      361 tcgcgagtat caatctgctc acccatattt ttcagtttct gggtgtcata cgccgtcaat
      421 ccgacaaaga caatcacgcc gatgtaggtg actgcccaca tcaatgcttc gcttttcagc
      481 cagaagttaa ccagcgatgc cagcacaatg ccgattaacg ccataaacag catattgcca
      541 aagccgctta gatcgcgctt cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg
      601 gcagtaacga cgaaagtact ggcgatagaa gcagcggtat agacaatgaa aatactggaa
      661 agcgtaagac ccgtcagcgc cgaataaagc ataaagagca tcgtcgttac acctgcgctc
      721 agcttttgaa tcatcgctga taacacaata accaatgcta attgcgcgat gatcagaccg
      781 attaaaaaga cacggttagt aaacaacagc tccatcacgg ccgcggaatt agccgcatac
      841 caggcaacaa atgctgtcag caacaagcca acggtcatcc agccatagac ttgcgccata
      901 taagtttgca agccagcccg gggttgtacg attgaatcag aacgtgggaa tctgtccatg
      961 actttctcct gaagatataa ggaatatctt aaggatactg caaaatgatg aggctgtgca
     1021 tcgacgcagc gtaaacggat gtactgagcg gtgaaattgc cggacgcagc ggtgccttat
     1081 ccggctaaca ataaactacc agcgttttgc cgcctgctga tcgctctccc gagcttcgac
     1141 ccagcggtcg ccttccggcg tggcttcgcg cttccagaac ggtgcgcggg ttttgagata
     1201 atccataata aactgcccgg cttcaaacgc actgctgcga tgcgcactgg taacaccgac
     1261 aaaaacaatt tcatcgcccg gccataattc accaatgcgg tgaattaccg tgacgcgccc
     1321 cagcggccag cggttacgcg cttcatcaac gatttctgcc agtgcttttt ctgtcattcc
     1381 cggatagtgt tcgagggtta atgctttgac gctgtcgccc aggttatggt tgcgcacctt
     1441 accggtaaag gtgactaccg caccgtcttc gtcacgctca gccagccacg ggtactcttc
     1501 tcctacgctg aacggctgcg ggccgacaac aattttggtt tctgccatct taacctccgg
     1561 ttaccggcgg gaagaaagcc acttcgtcgc cgtcagtcag cgaatggtca aaactcacca
     1621 gcgtctggtt gacagcagcc agcaatttgc catcttccag cgccagagcc cagcgatcgc
     1681 tctgcgcagc cagatgctgg cgtaacgctt caacggttgg gaaatccgca gccacttcgg
     1741 ttgcatcagt tcccaccaac tcgcgcacct gggcgaaaaa aagaacgtta atcatccgct
     1801 tccaccttaa agtcacccga cttgccgccg ctctttgcca gcaaacgtac cggaccaatc
     1861 accatatctt tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc cacggaggcc
     1921 gcggttaacg cttccatttc gacaccggtt ttcccggtca ggcggcataa ggtttctata
     1981 cgtacccgat tgtgctccgg ctcggcctgt aaattgactt caactttgct gagcatcagc
     2041 gggtgacaca gcgggatcag atcccaggtg cgttttgccg cctgaatacc ggcgatacgc
     2101 gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg
     2161 ctgcgcatgg tgacaaaggc ttcggcgcgc gcttcacgca cggtttccgc tttggcggag
     2221 acatcgacca tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga catacttatt
     2281 tcttcaaatg tggatggaaa ttacacggac gcgtacgggc atccagctgc ggcgcaatga
     2341 tattttccca tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa atcagcgttt
     2401 tgttagcgac gcccgctacc gcacgagatt gcaacgtgga agtgccaatc tcttcgaacg
     2461 acaacatccg gaacacttca ccaaaacctt caacttcacg gtcgaacaac ggcagcagcg
     2521 cttcgggagc ctgatctcct tccgtcaggc cagtaccacc cgtaatcaac accacttgta
     2581 catcgtcgct ggcgatccac gcagagacct gagcgcgaat agcgtagcgg ttttctttca
     2641 caatggcttt atcgacaacg tgatggcccg cttcttgcgc cgagtcgcga agatagtgac
     2701 cggaggtatc gtcttcttca ccgcgacgat tagaaaccgt aagaatagca atacgggtcg
     2761 ggataaattc agtgcttacc tgactcatct gatctctcct tttgacgttt tagccaccaa
     2821 tgtacgataa gttttgcgta ataccggtgt tattttgatg caggaaatgg gtctgttttt
     2881 tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg ttgctgggta tcgttttcca
     2941 gcagatcgcg caggttaacg ccgccttcac caaacaggca gagatggagt ttaccaatgg
     3001 aggaaacgcg caggcggttg caagtggcgc agaaatcttt ttcatacggc atgataaggc
     3061 caatctctcc ggcgtaatcc ggatgacaaa agacttgcgc gggaccgtcg ctgcgttgac
     3121 gtaattggtg gatccagccg cgacgcagta actcgtcacg cagaacctga ccagagatgt
     3181 gatgcttacg gaagagctcg ctgccctcgc ccgtttccat cagttcgatg aaacgcagct
     3241 ggatgggacg atgctggatc cagttcagaa aggtgtcgag ctggtgatga ttcacatcac
     3301 gcatcagcac ggtattgact ttgaccttct caaaaccggc ctcaaatgca gcatcgattc
     3361 ctgccatgac ctgattgaat ttatcctgcc cggtaatggc atgaaactgg cgagcgtcaa
     3421 ggctatcaac gctgacgttg atgccagtaa gtccagcatc gcgccagttc gccacatcgc
     3481 gctccagacg gtaaccattg gtggtgaccg ctatctggcg aatagcgtcg ttttctcgca
     3541 cagcggcgat gatatcggta aagtcgcggc gtaaagatgg ctcaccacct gtcagacgga
     3601 ctttttcggt gcccagactg gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtca
     3661 ggaagccttt attggtgacg ccgctcggct tgtaaccatc cggcaggcag taggtgcaac
     3721 gaaagttaca cacatcagta atcgacaggc gcaagtagta aaacttacgc gcaaatgcat
     3781 cagtcagttg tgaagccatg tacacctttc cagatacggg aggcgaagtc atttcttcct
     3841 tcgccctggt ggcaatcttt acgagcattg tcacggccaa agcaccgtat cagttgaccc
     3901 aggtgcagag gctagagtgt ttagtggtta agccgatact agcgtgtaaa tgagattttt
     3961 accatccaca ctttcgctat gtaatcatgt atatagcgtc atgatcgtgc tatttcgcta
     4021 cagaataagg gaaaatggcg gttttacttt gatatacatc atcagggagg catagcgtgc
     4081 atcgtccttt agaggtagaa gaaaatagtt tgtatcctca atattggcag gttaattgct
     4141 gtttcctcgc aatttgcgct actgtagcgc ggataatctg actccaggaa actatatgcg
     4201 caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc atggactggg
     4261 acgcgttctc tcatcacttt cgtctttggg ttctcgttta acgggtatcg tcaccaccac
     4321 cgataacggt ggctcgacgg ggcgtattcg ccgctcagaa ggcggcattg cctggggcga
     4381 tatgcgcaac tgcctcaatc agctgataac tgaacccagc gtcgcttccg caatgtttga
     4441 atatcgtttc ggcggtaatg gcgaactttc cgggcacaac cttggaaacc tgatgttaaa
     4501 ggcactggat caccttagcg tgcgacctct tgaagcgatc aatctgattc gaaatctgct
     4561 gaaagtggac gcacatttga ttccgatgtc ggagcatcct gttgatctga tggcgattga
     4621 cgatcagggg catgaagttt acggcgaggt caatatcgac cagttaactg cgccgattca
     4681 agagttattg ttaacgccga atgtacccgc aacgcgtgag gcggttcacg ctatcagtga
     4741 agcggatctc atcattattg ggcctggcag tttttacacc agcctgatgc caattctgct
     4801 gctgaaggaa atcgcacagg cattacgccg cacgccagcg ccgatggtct atatcggcaa
     4861 tctggggcgt gagttgagtt tacctgcggc taatctgaag ctggaaagca agctggcaat
     4921 tatggagcag tatgttggta aaaaggtaat tgatgcggtc atcgtcgggc caaaagtgga
     4981 tgtctcggcg gtgaaagagc ggattgtgat tcaggaagtg ctggaggcca gcgatattcc
     5041 gtatcgtcat gaccgccagt tgttgcatag cgcgctggaa aaggcattac aggctttagg
     5101 ttaacacgaa gccggaagaa tattccggct tcgtcatctt gtcacgacgc ggcaataaac
     5161 agctcacgca actgatgcaa ctggtcacga atttgcgccg cttcttcgaa ctccagattc
     5221 tgcgcgtgtt gcatcatcaa cccttccagt tcatggatct tctgctgtaa cgctttaggc
     5281 gacatatcca tcggcacatt atccggctca acaatcgggc gcgattttcc tctgcccttc
     5341 gctttggttt tggcaatgtt ctgccccagc gccaggatat cgaccacttt cttgttcaag
     5401 ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcgcg gcgacgttcg
     5461 gtttcgccaa tcgctttcgc cattgatggg gtgatcttat cgccgtagag aatcgcttta
     5521 ccgttaacgt tacgtgccgc acgaccaatg gtctggatca acgaacgttc ggaacgcagg
     5581 aagccttctt tgtcagcgtc gaggatcgcc accagcgaaa cctccggcat atccagacct
     5641 tcgcgcagta agttgatccc taccaatacg tcgaactcac ccagacgcaa gtcgcggata
     5701 atctccatac gttcgacggt atcgatatct gagtgaagat aacgcacgcg ctcaccgtgt
     5761 tcttcgagat attcagtgag atcttccgcc atccgcttgg tcagagttgt aaccagtacg
     5821 cgttcgttaa ttgccgctcg ctgacgaatc tccgatagaa gatcatccac ctgtgtcgct
     5881 accggacgca cttcaataat cgggtcgagt aagcctgttg gacgcacaac ctgatccacc
     5941 acatcgccac cggatttttc cagctcgtaa ttacccggcg tcgccgaaac atagatggtt
     6001 tgcggcgcta atgcttcgaa ctcttcaaat ttcagcgggc ggttatccag cgctgatggc
     6061 aagcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg
     6121 ccaatttgtg gaatggtgac gtgagattca tcgaccacca gcagcccatc agcaggcagg
     6181 taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag
     6241 ttctcaatcc ccgaacagta gcctagctcg ttcatcatct ccagatcaaa ctgggtacgc
     6301 tgggtcagcc gctgctcttc cagcagtttg ttgttttcca atagcacttt acgtctggcg
     6361 gccagctctt ctttgatctc ctccatcgcc tggacgatgc gctcgcgtgg cgtgacgtag
     6421 tgcgttttcg ggtagatggt aaaacgtgga atagtggaaa caatctgccc ggtcagcggg
     6481 tcaaataacg acaatcgttc cacctcctcg tcaaacagct ccacgcgaag tgcaatgtca
     6541 tccgattctg ccgggaagat atcaatcacc tcgccacgaa cacggaaagt accacgctgg
     6601 aatgcttgat cattacgggc atattgcagc tccgccagtc ggcgcaggat cgcgcgctga
     6661 tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc
     6721 agaccataaa tcgcggaaac agacgccacc acaaccacat cacgtcgctc cagcatcgct
     6781 ttggtggcgg ataaacgcat ctgttcgata tgttcgttta ccgaggcatc tttctcaata
     6841 aaggtgtcgg aactcggtac ataggcctcc ggctgatagt agtcgtagta ggagacgaaa
     6901 tattccaccg cgttttctgg gaagaattct ttcatttcgc catacagctg ggccgccagc
     6961 gttttgttgg gcgcaagtac catggttggg cgctgaaggt cagcaatgac attggcaatg
     7021 gtgaaggttt tcccggagcc agtcacgcca agtaacgtct ggtgcgccag gccatcttcc
     7081 agcccctctt cgagacgtcg aatcgcctcc ggctgatcgc cagaaggttt aaaagcggaa
     7141 ttcagtttga acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt
     7201 aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa aaca
//
LOCUS       DAPQZD010000212         7239 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPQZD010000212 DAPQZD010000000
VERSION     DAPQZD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.214"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..855
                     /locus_tag="RO454_004482"
     CDS             <1..855
                     /locus_tag="RO454_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDY0936208.1"
                     /translation="TGSQTNINAQIVKNIKIPIPCPDNPEKSLAIQSEIVRILDKFTA
                     LTAELTAELNMRKKQHNYYRDQLLTFKEGEVEWKALGEIGEFIRGKRFTKADYVEDGG
                     ISVIHYGEIYTRYGVYTTHSLSQVRADMAASLRYAKHGDVVITDVGETVEDVGKAVAW
                     LGDDDIAIHDHCYAFRHSLNPKFISYYMQTDSFISEKAKYVARTKVNTLLINGFSKIM
                     IPVPYPKDHEKSLKEQARIVEILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSF
                     PKPETVSN"
     gene            977..4093
                     /locus_tag="RO454_004483"
     CDS             977..4093
                     /locus_tag="RO454_004483"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDY0936209.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDL
                     RNQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKINSSYKAKNWSTPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVAELEQRFPDPASIESEKEKKDFVKLFGEYLRTENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKLAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQHEQLREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIVSFIEK
                     FKGVGGKI"
     gene            4307..5004
                     /locus_tag="RO454_004484"
                     /pseudo
     CDS             4307..5004
                     /locus_tag="RO454_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5141..6073)
                     /locus_tag="RO454_004485"
     CDS             complement(5141..6073)
                     /locus_tag="RO454_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936210.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            complement(6077..7072)
                     /locus_tag="RO454_004486"
     CDS             complement(6077..7072)
                     /locus_tag="RO454_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDY0936211.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
BASE COUNT         2048 a         1663 c         1674 g         1854 t
ORIGIN      
        1 acagggtccc agacaaacat aaatgcgcag attgtcaaaa atattaagat ccccatcccc
       61 tgcccggata atccggaaaa gtcccttgcc atccagtctg aaatcgttcg gattctggat
      121 aagtttactg cacttaccgc tgagcttacc gctgagctta acatgcgtaa aaaacagcac
      181 aactactatc gcgaccaatt gctgactttt aaagaaggtg aggttgagtg gaaagctttg
      241 ggggaaattg gtgagtttat tcgtggaaaa cgttttacta aagcagatta tgtagaagat
      301 ggtggtatca gtgtaatcca ttatggtgag atatatacac gatatggtgt ttacacaact
      361 cattcgttat cacaggttag agctgatatg gcagcgtcat tacgctatgc caaacatgga
      421 gatgtcgtga taactgacgt gggggagact gtagaagacg ttggtaaagc agtggcatgg
      481 cttggggatg acgatatcgc tattcatgat cactgttatg cttttcgaca ttctttgaat
      541 ccaaaattta tttcctatta catgcaaacc gattcgttta tttctgaaaa agcaaagtat
      601 gtagccagaa caaaagttaa tacactactc attaatggtt tttcaaaaat tatgattccg
      661 gttccatatc ctaaagatca cgaaaaatca cttaaggaac aagctcgcat tgtcgaaata
      721 ctcgacaaat tcgacacact gactaactcc atcaccgaag gtcttccgcg tgaaatcgag
      781 ttgcgccaga aacaatacga atactaccgt gatttactgt tcagtttccc aaaacctgaa
      841 actgtcagta attaattgac cgttgctacc gaccgggcca ccttaacatc cggtctgtat
      901 atagactatt ttttacgtgc cggaagtcac ccttaactcc cttccggccc ttgccagacg
      961 gcacaaagga tgcgctatga ctcatcagac acacaccatt gctgaatcca ataactttat
     1021 cgtccttgat aagtacatca aagctgagcc aacaggcgac agctaccaga gcgaatcgga
     1081 cctggaacga gaactgattc aggacctgcg gaatcagggc tatgaattta tatccgtaaa
     1141 atcacagtcg gcgatgctgg ccaatgttcg ggaacagctt cagaacctca atggtgtggt
     1201 gtttaatgac agcgagtggc ggcgtttcac ggagcagtat ctggataacc cgagcgatgg
     1261 cattctggat aagacccgta aaatccatat cgactatatt tgcgacttta tttttgatga
     1321 cgagcgtctt gagaacatct atttgataga taaaaagaat ctcatgcgca ataaggtgca
     1381 gattatccag cagtttgaac agacgggttc tcatgctaac cgttatgacg tcacgatcct
     1441 ggttaatggt ttaccgctgg tgcaaatcga actaaaaaaa cgcggggtgg cgattcgtga
     1501 ggctttcaac cagatacatc gttacagtaa agagagtttt aacagcgaaa attccctgtt
     1561 taagtatctg caactgtttg tcatttctaa cggcaccgat acccgttatt ttgccaacac
     1621 gacaaagcgc gataaaaaca gttttgactt caccatgaat tgggcaaaat cagacaacac
     1681 gctgattaaa gacctcaaag actttaccgc tacctttttc cagaaacata ctctgctgaa
     1741 tgttctggtg aactacagcg tttttgacag tagccagacg ctactggtga tgcgaccgta
     1801 ccagattgcc gccactgagc gtatcttatg gaaaatcaac agctcctata aagcgaaaaa
     1861 ctggtctacc cccgaaagtg gtggctttat ctggcacact accggttctg gtaaaaccct
     1921 gaccagcttt aaagccgcgc gtctggcaac agaactggac tttattgata aagtcttctt
     1981 tgtggtcgac aggaaagacc tcgattacca gaccatgaag gaatatcagc gtttttcgcc
     2041 agacagtgtc aatggatcgg aaaataccgc aggtcttaaa cgaaatctgg ataaggatga
     2101 taacaaaatt atcgtcacca ctattcagaa actcaataac ctgatgaaag cagaaagcga
     2161 cctgcctgta tacaatcagc aagtggtgtt tatatttgat gaatgccacc gcagccagtt
     2221 tggagaagca cagaaaaacc tgaagaagaa attcaaacgc tattatcagt ttggttttac
     2281 cggtacccct attttcccgg aaaacgcctt aggctcagaa acaaccgcca gcgtatttgg
     2341 tcgtgaattg cattcatatg ttattaccga tgcgattcga gatgaaaaag tgcttaaatt
     2401 caaggtggac tacaacgatg tgcggccaca gtttaagtct ttagagacag aaaccgacga
     2461 gaaaaaactg agtgcggctg aaaatcagca ggcgtttctt catcccatgc gtatacagga
     2521 aattacgcaa tatattctga ataatttccg ccagaaaact caccgtacct tccctggctc
     2581 aaaaggtttt aatgctatgt tggcagtgag cagcgtggat gccgcgaaag cctattatgc
     2641 gacgtttaaa cggttacaag aagaagccgc taataaatcg gctacctata aaccgctgcg
     2701 tattgcgaca atcttctcct ttgccgccaa tgaagaacaa aatgccattg gtgagatttc
     2761 cgatgaaact tttgatacca gcgcaatgga cagcagtgct aaagagtttc tggacgctgc
     2821 aattcgtgaa tataacagcc attttaaaac taactttagc accgacagta acggttttca
     2881 gaactactat cgtgatttag cccagcgggt taaaaatcag gatatcgatc tgctaattgt
     2941 cgtggggatg ttcttaaccg gcttcgatgc tccaacattg aacacgctat tcgtcgataa
     3001 aaacttacgt tttcacggcc tgatgcaggc attttcccgc accaaccgca tttatgacgc
     3061 cactaaaacc ttcggtaata tcgtcacttt ccgggatctg gaacgctcaa ctattgatgc
     3121 cataacgctg tttggtgaca aaaacaccaa aaatgtagtg ttagaaaaga gttatgcaga
     3181 gtatatggaa ggctttactg atgctgctac tggtgaagct aagcgcggct ttatggcagt
     3241 agttgcagaa ctggaacaac ggttccctga ccctgccagt attgaaagtg aaaaagagaa
     3301 gaaagacttc gtaaaactgt ttggtgaata cctgcgtacc gagaacatcc tgcaaaacta
     3361 tgatgaattt gccacgctga aagccctgca acaaatcgat cttagcgatc ctgttgcagt
     3421 agaaaaattc aaagcagaac attatgtgga tgatgaaaag ctcgctgaat tgcaaacaat
     3481 tcgtctccct gctgatcgca agattcagga ttatcgttct gcctataacg atattcgcga
     3541 ctggcagcgc cgtgagaaag aagctgagaa aaaagagaaa tcaaccactg actgggatga
     3601 cgtggttttt gaggtcgatt tgctgaagtc tcaggaaata aacctggatt atatccttgg
     3661 actgattttc gaacataaca gacaaaataa aggcaagggc gaaatgatcg aagaggtcaa
     3721 acgcttaatt cgttcaagcc tggggaaccg ggcgaaagag ggcctggtgg tcgattttat
     3781 tcagcaaacg aacctggatg atttaccaga caaagccagc atcattgacg cgttctttac
     3841 ctttgctcaa cacgaacagc tacgtgaagc agaagcgttg attaaagaag aaaatctcaa
     3901 tgaagaggcg gcgaaacgct atattcgcac gtctttaaaa cgcgaatacg ccaccgaaaa
     3961 tggcacagaa ttaaacgaga cattaccaaa acttagtccg ttaaacccgc aatataaaac
     4021 gaaaaaacag gcagttttcc agaaaatcgt ctcgtttatt gagaagttta aaggcgtagg
     4081 cggaaaaata tagcccaatt cgtgtttttc ttgcgggttc ttaattaaac ccgcaagaga
     4141 ccgtggggtt ccaaatggct aatatactcc ccttacccat gagcgacgat gccgccaaaa
     4201 gtgatagaga acagccagaa atagatcgcg gccataatga ttttgaatgc cggtgatgct
     4261 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctcctgtgg cttctgtttc
     4321 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcactgc
     4381 cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta
     4441 caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg
     4501 atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa
     4561 aaactcaggc tgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc
     4621 gcggaaatgg acgaacagtg gggatacgtc ggggctaaat cgcgccagcg ctggctgttt
     4681 tacgcgtatg acaggctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg
     4741 gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg
     4801 gatggctggc cgctgtatga atcccgcctg aaaggaaagc tgcacgtaat cagcaagcgt
     4861 tacactcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
     4921 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
     4981 ctgaacataa aacactatca gtaagttgga gtcattacct gtgttactaa ccgtctgagg
     5041 cgaaggtgac tatgggttta cgctgacact caggcaacca agaattgttt atcggatgtg
     5101 ctccggcata cgtaaatcct acaccggagc taaggaggga ttacttctga aggtgcagct
     5161 ccttcagatt tgtttcaaag taatcaacat aaattcgttc atttttgggc ttcattatca
     5221 gctgaatacc gttgggtacg ggcggacatg aggcccggta ggtaaacatc tcatcttgga
     5281 tattccggag cgtataagcc agttgctctt cttctgccct tccggacacc cttatgccgg
     5341 ataattcctt ctcaacgtac tttttcagct tctcgcttct tttcatactg ctgcgattat
     5401 ccagaaactg aagcaagaaa tagcggatga ccggcagtgt gaacaccaca tagaagagga
     5461 tctcattcag cccggatttc atgacgatgc cgtaaacaag catcgcgaca ggcgagacaa
     5521 taaaagcgga aagataaaat acgtgcagaa cctccctgag cgatttgtcc cagccgagat
     5581 tttttccgtg gcaaaggagc gccattacgg cctgcggcgc gtcatacacc ttactgacgt
     5641 accacccctc ttcgccaaat cgctttcgtg ctttttccgg gctgtctcta aaatatgcag
     5701 cttcccggat gatgtcatcc cgtgggatct cctccacctt cagttcatta cgccggatgc
     5761 caagtacccg gcagtcgtac atttcctgga ttgaggctgc cttcctgcgc agagccgaga
     5821 tgtgtccact gataacaatg ttaaaggtaa gcaccaggag tccatataac cccaacagcg
     5881 aagacaacaa ctctgaatcg acaaattccc ggctccggtt gtatacggta acgaaagaaa
     5941 gcaggagcgg tacgatgatg ctgaagagaa aattcagtct gttccagttt tttaccctgt
     6001 cgtagacacg ggcctgcgca tacagtaaat ccatgttttc tctggtcgac tggtttgtgt
     6061 aaatggcatt catgattcac ccgtaaacag gaaagaatgg cccgaacaca tcgcgccatt
     6121 tattgatgga tttttcatac tctttattat tttcaaacca tctcgcctcc gatacctttt
     6181 gcgcatcgag ataacatctg tccgatatcg ccttacgtgc ctcggctgaa agtgagttga
     6241 tatcaccctg tatgcctttg ggatcgttga cagagtaccg cacggattga ccaaggtggc
     6301 ggaaaagcgc ttccagctcc atgtcaacaa acgatgagca ggatgtcctg ccagcgtaat
     6361 agtccagaat cagcgtttcc agcaggtagg aactcatgga tggcatcgtc ggtcgccgct
     6421 gccagtattt gactgctctg atgacattga gcacattgcc gttattctgc acattgatag
     6481 tagtgactct gtccctgtct ttacgggggt cggtaaactt ccagtggccg ttgccatcgg
     6541 ggatcaggta gtaagtccgt ccaaaggcat cttcgctggt aataaagcag gggacgatgt
     6601 caaaattcca gtccttgctg accagtttca gcgtgacagc ttcctggtta cgcctgatat
     6661 cagcctgggc gtactgggca atgtcctgca ggcggttttt aaaggcattc agaattctga
     6721 cggagcagac ggtgtcagct ccggaatggc ggtaactgtg cagacgtgac ccctctccgg
     6781 aggaggttac agttatccgg tcactcagga tggtataggt ggcaccttgc ccggttaagc
     6841 caaacatcag atcgatatca tcaagcggac ggattttggt tcttctggca aaggagccaa
     6901 aggcaatatg gatcgctgga aaactcaccg gaaatttatc gtccttctca aaatcattca
     6961 tcttaccgat aagccagtcg cgacttgcac gcgcgtcatc ggtatctgcc tttttgagat
     7021 tcacggtatc tttcataaat tcgttaaagg cagctattac tgtggtcgcc atgttcagtc
     7081 ctttaattat ttattgcaaa aggcgcgggg ctctgtcgta catctgaaca ttccagtgcc
     7141 cgggaagagt cggtacagtt tattattagt gccgcaagtt cactaccgtc tcgctctctg
     7201 caagggcata taatttacat gggaaatatg agcctcgtt
//
LOCUS       DAPQZD010000213         7055 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPQZD010000213 DAPQZD010000000
VERSION     DAPQZD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.215"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            95..1081
                     /locus_tag="RO454_004487"
     CDS             95..1081
                     /locus_tag="RO454_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDY0936212.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETRDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            1096..1437
                     /locus_tag="RO454_004488"
     CDS             1096..1437
                     /locus_tag="RO454_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDY0936213.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPSMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            1442..3088
                     /gene="fliF"
                     /locus_tag="RO454_004489"
     CDS             1442..3088
                     /gene="fliF"
                     /locus_tag="RO454_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDY0936214.1"
                     /translation="MNAQIKKLTQAFPAFRLHLVDNKRWVLMAGVGLAVAVTAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            3066..4076
                     /locus_tag="RO454_004490"
     CDS             3066..4076
                     /locus_tag="RO454_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDY0936215.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINHFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            4080..4790
                     /locus_tag="RO454_004491"
     CDS             4080..4790
                     /locus_tag="RO454_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HDY0936216.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            4783..6123
                     /gene="fliI"
                     /locus_tag="RO454_004492"
     CDS             4783..6123
                     /gene="fliI"
                     /locus_tag="RO454_004492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDY0936217.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTRLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            6126..6560
                     /gene="fliJ"
                     /locus_tag="RO454_004493"
     CDS             6126..6560
                     /gene="fliJ"
                     /locus_tag="RO454_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDY0936218.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            6563..6958
                     /locus_tag="RO454_004494"
     CDS             6563..6958
                     /locus_tag="RO454_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HDY0936219.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGEQLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRGHF"
BASE COUNT         1676 a         1915 c         2080 g         1384 t
ORIGIN      
        1 tatttttatt atcgtttaat tcgaacttgt taatcccacc ggtgatacgc ttcagaacat
       61 cctgttttct gtcgctactt atatcgttca ccctatgtct gaacttattg caactgccgc
      121 ttccagcatt aacgctttta ctctggcaaa gcgtgtcgcc gcctttaacg taccggtgct
      181 cattcagggc gaaaccggcg cggggaaaga gtgcgtggcg aaatatattc acaccgtagc
      241 ctttggtgaa aatgataacg cgccctatat cggcgtgaac tgtgcggcga tcccggaaaa
      301 tatgctggaa gcgaccttat ttggctacga caaaggcgca tttaccggcg caattgccag
      361 cgtgccagga aaaatggaac tggctaataa cggcacctta ttgctcgatg aaattggcga
      421 tatgccgctg gcattacagg ccaaaatatt acgcgtattg caggaacagc aggttgagcg
      481 attaggcagc aatagacaaa tcaaactcaa ttttcgcctg attgcctgca ccaataaaaa
      541 tctggaacag gacgtggctg ctggacgttt tcgtgaagat ctttattatc gtctggcggt
      601 tattcctata accatgccgc cactgcgtga acgtctgaac gatattattc cgctggcaga
      661 gtcgtttatt aaaaaatatg ccacggtgct ggtgaaaaat atcaccctgt ccgaatccac
      721 acgccgggcg ctacttaatt accgctggcc cggaaatgtt cgccaactgg aaaacgccat
      781 tcagagagga atgatcttaa accgcgacgg cgtgatttac ccggatgcct taggcctgcc
      841 ggaaactgac attgccgacc acagcgaact gcaatggcct gttcagcccg ccgtccacat
      901 tgccgaaacc cgcgatttag gccagcacgg acgaagtgcg cagtatcaat atatcgccga
      961 cctgatgcgt aaatatcagg gcaaccgcag caaaatcgcc gacctgttag gcattacccc
     1021 gcgcgcactg cgctatcggc tggcctccat gcgcaagcaa ggtatcgaag ttttctcctg
     1081 acccggaagt taaaaatgag cattaccaca atcaacagca ccatgcaggc gcaaatcatg
     1141 caggacgtgc agcggatgca ggccaacgcc caggcgccgg tgctaccctc catgaccttc
     1201 agttccacag acccggacgt gtcgtttaac cgcattatgt acggcgcgct tggtcacgtt
     1261 gatcagttcc agcaggtggc ggagcagcag caaacggctg tcgacacggg caaaagtgat
     1321 gacctggcag gtgcgatgat cgccagccag caggcgtcgc tctctttctc cgcgctggtg
     1381 caggtgcgca ataaaatcgc caccgggttt aacgacctga tgagtatgtc gatctaaccc
     1441 tatgaatgca caaataaaga agttaaccca ggcgtttccg gcgtttcgac tgcatctcgt
     1501 agataacaaa cgctgggtgt tgatggcggg agtggggctt gccgtggcgg tgacggcgat
     1561 tatcgtcagc gtgctgtgga ccggtaatcg cggctacgtt tcgctctacg gacgccagga
     1621 aaatctgccc gtgtcgcaga ttgtcacggt gctggacggc gaaaagctca cctaccgcat
     1681 cgacccgcag agcgggcaga ttctggtgcc ggaagatgca ctgtcgaaaa cgcgaatgac
     1741 ccttgccgcg aaaggcgtgc aggcgatatt gcccagcggc tacgagctga tggataaaga
     1801 cgaagtgctc ggcagcagtc agttcgtgca gaacgtgcgc tataaacgca gtctcgaagg
     1861 ggagctggcg cagagcatca tgtcgctgga cgcggtggaa agcgcgcggg tgcatctggc
     1921 gctcaacgaa gaaagctcgt ttgtggtcag cgatgagccg caaaacagcg cctcggtggt
     1981 ggtgcgcttg cattacggcg cgaagctgaa tatggaccag gtgaacgcca ttgtgcatct
     2041 ggtgtccggc agtattccgg ggctacaggc cagcaaagtt agcgtcgtcg atcaggcggg
     2101 aaatctgctg accgacggca ttggcgcagg cgaggcggtt tctgccgcga cccgtaaacg
     2161 cgatcagatc ctcaaagata ttcaggacaa aacccgtgcc agcgtggcga acgtgctgga
     2221 ttcgctggtt ggcacgggga attaccgcgt cagtgtgatg ccggatctcg acctcagcac
     2281 catcgacgaa acccaggaac actacggtga cgcgccgaaa atcaaccgcg aagagagcgt
     2341 gctggacagc gacaccaatc aggtggcgat gggcgtgcct ggttcgctca gtaaccgtcc
     2401 gccgattgca gcgaatcaga tgaccaacgg tacggaagaa aaccgctcgc cggaagcgct
     2461 ttccaaacac agcgaaagta agcgcgacta ctcttacgac cgcagcgtcc agcatattca
     2521 gcatcccggc ttcgcggtga aacgcctcaa cgtggcggtg gtactcaatc aaaacgctcc
     2581 ggcgctgaaa aactggaagc cggagcaaac cacgcaactg accgcgttgc tgaacaatgc
     2641 cgccgggatc gacgcccagc gtggtgacaa tctctcctta tcgttactca actttgtacc
     2701 gcaggcggtg cctgtcgaac cggttattcc actgtggaaa gatgacagcg tactggcctg
     2761 ggtgcggctg attggctgcg gcctgctggc gctgttgctg ctgttcttcg tggtgcgtcc
     2821 gttgatgaaa cggctgacgg cggtacgtgc gccggttatc acaccagaac cggaagccgt
     2881 cagcgaaccg tggattgcca tgccggaaga ggagcgcaaa aacgtcgacc tgccgtcgct
     2941 gcccggcgat gacagcctgc cgtcacaaag ttctggcctc gaagtgaaac tcgagttcct
     3001 gcaaaaactg gccatgagcg acaccgatcg cgtagccgaa gttctcagac aatggatcac
     3061 cagcaatgag cgaattgaca acaaataaga gcaacagcta cctggaacag gcggcgatcc
     3121 tgctgctgtg tctgggcgaa gaggcagcgg cgacggtgat gcaaaaactc agccgcgaag
     3181 aagtggtgcg cctgagcgaa aacatggcgc gactttccgg cgtcaaaacc agcatggcgc
     3241 gtaaagtgat caaccacttc tttgacgagt tccgcgaaca gagcgggatc aacggcgcat
     3301 cgcgatccat gttgcagggc attcttaaca aggcgttggg taccgagatc gccagcagcg
     3361 tgatcaacgg catctatggc gatgagattc gctcgcggat ggcgcgcctg caatgggtgg
     3421 aaccacgcca gttggcgatg ctgatttccg aagaacatct gcaattgcag gcggtgttcc
     3481 tcgcgtttct gacgccggaa atctcggcag cggtgctctc ttatctcaac gaatcggtgc
     3541 aaaacgaaat tctctaccgg gtggcgaaac tcaacgacgt caaccgcgac gtggtggatg
     3601 agctggaccg cctgattgaa cgtggcctgt cggtactttc ggaacatggc tcgaaggtaa
     3661 aaggcatcaa acaggcggcg gatatcgtca accgcttcca gggcaatcag caggtgatcc
     3721 tcgaccagat gcgcgaacgc gacgaagagg tgttggggca gatccaggat gagatgtacg
     3781 acttctttat cctcagccgc cagaacgaag aagtgcgtcg gcgtctgctt gatgaagtgc
     3841 cgatggaaga ctgggcggtg gcgctgaaag gcaccgaagc cctgctgcgc cgctcgattt
     3901 acgcggtaat gccaaaacgt caggcgcagc aactggaagc gatcaccgcg cgtctgggtc
     3961 cggtgccggt tagccgtatc gaacagatcc gccgcgaaat catgggcatc gcccgtgagc
     4021 tggaagaagc gggcgaaatt cagctacagc tctttgcgga acggacggcg gagtaattca
     4081 tgaccattga aacactgcgc gggcgctatc gcctgcaccg cttcccgccg cgccagcgtg
     4141 cggtgaccgg agaaccgctt actgccagta tcagcgccgc cgattaccag cgtcagttga
     4201 tggacggttt tcaggaaggg ctgcaaaaag ggtttgctca gggaatgacc gaaggtcagg
     4261 agcagggctt tagcgaaggt catcaacaag gttttgccga ggggcggcgt cagggctaca
     4321 ccgaaggcag cctcgccggg cagcaggaag gacgcaaaca gtttgttgag gcggcgcagc
     4381 cgctggaagc catcaccggt aaagtgaacg acttcctcgc gcatattgaa cgtaaacagc
     4441 gggaagattt gctgcaactg gtggagaaag tcacccgtca ggtgatccgc tgcgaactgg
     4501 cgctgcaacc gacgcagtta ctggcgctgg tggaagaggc gctggcggcc ttcccgacga
     4561 tgccggagac cctgcaagtg atgctgagta cagaagagtt taaccgcctg cgcgacgcgg
     4621 tgccggaaaa agtcagcgaa tgggggctga cgccttcgcc ggatctgccg ccaggcgaat
     4681 gccgggtgat taccgacaaa tctgaactgg acatcggctg tgagcatcgc cttgaacagt
     4741 gcatgaccgc gctgcaagag acgctaacgc cggagtcgca gggtgagtga tttttctgct
     4801 ttcgataacg ccctgcgctc gctggagtcg atcccgctgg cccgcgtcgc cgggcggctg
     4861 gtgcgcctga acggcattct gctggagagc gtcggctgcc cgctaatgac cgggcaactg
     4921 tgccgcatcg aaagcgccaa tcacacactg atcgacgcgc aggcagtggg ctttaatcgc
     4981 gacatcacct acttaatgcc gtttaaacag ccggtcggcc tgatggcagg cgcacgcgtc
     5041 ttcccggaag aaaaagccca cgacatcctg attggcgaaa gctggttagg ccgcgtggtt
     5101 aacggtctcg gcgaaccgct ggatggcaaa ggccgcctga acggcaacga ccttctgccg
     5161 ccgctgccac cctcggtcaa tcccttaacc cgccgttcag tggacgaacc gctggacgtg
     5221 ggcgtgaaag cgattaacgg cctgctgacc atcggcaaag gccagcgtgt cgggctgatg
     5281 gcgggcagcg gcgtcgggaa aagcgtgctg ctggggatga tcacccgcca gactaaagcc
     5341 gacatcgtgg tggtcgggtt gattggtgaa cgcgggcgcg aagtgaaaga gtttatcgac
     5401 cactcgctgg gcgcagacgg gctagcaaag tctatcgtcg ttgtcgcgcc tgccgatgaa
     5461 tcgccgctga tgcgcctgaa agccaccgaa ctgtgccact ccatcgccgc ctggtttcgt
     5521 gaccgcgggc atcacgtttt actgctggtg gattccctga cccgctacgc catggcgcag
     5581 cgtgaaattg ctctctcact cggtgaacca cccgccacca aaggctatcc accatcggca
     5641 ttcggcatga tccccaaact ggtcgagagc gcaggcaaca gcgaaagctc aggatcgatg
     5701 accgccatct acacggtgct ggcggaaggc gacgaccagc aagatccaat agtcgactgc
     5761 gcccgcgcag tgctggacgg acatatcgta ttaacccgca aactggcgga agcggggcac
     5821 tatccggcta ttgatatcgg ccagtcgatc agccgctgca tgaaccaggt cacccgcctg
     5881 gaacatcagc aatccgcccg cgcgttaaag caaaactacg ccgcctatat ggagatcaaa
     5941 ccgctgatcc cgctcggcgg ctacgtggca ggcgcggacg ccagcgtcga caaagcggtg
     6001 aaaatgttcc cggctatcga gcgatttttg cgccaggaga tgcgcgaacc cgcctccctc
     6061 gaactggtgc aaagccgcct gcaaatcctc tttcccatcg ccaaaaaggc agaagggcaa
     6121 taaccatgcg acaaatcatc gacaccctgg cgcagttgca gcgcctgcgg gataaatcgg
     6181 tcaaagacaa aaccgtcgag ctggcaaagc aaaaacagat ttgcgctggc tatgataaca
     6241 atatcaaagc attaggttac cttgtcgaga aaacatctgc gggcgcggcg gcgtcggtgg
     6301 agtcgctgaa aaacgtcagc ggctacaaag gcacactgcg caaggtgatc gcctggcaag
     6361 agcaggaaaa aacgctggcc aacatcaaag ctacgcgaat gcagaaaaac ctgacggcgg
     6421 cggcgtgcga agagaaagtg gtagccctga cgctggacga caaacgccgc gagcaacagg
     6481 aaagcgccac ggcgaaagcg caaaaagcgg tggatgacat cgccgtccag tgctggttgc
     6541 ggcacaaatt agcggagtag ccatgaaaac ggtcatcacc ttcggcacct tcgatgtgtt
     6601 tcatgtgggt catctgcgcc ttttacaacg cgctcgcacg ctgggcgagc agctgctggt
     6661 cggcgtatca tccgatgcct taaatatcgc caaaaagggc agggctccgg tttaccctca
     6721 ggatgaccgc atggcgatta tcgccgggct ggcgtgcgtg gatggcgtat ttctggagga
     6781 gtcgctggag cagaaagcgg aatatttgcg cgggtacagt gcggatattt tggtgatggg
     6841 cgatgactgg gcaggtaagt ttgacgattt ctcgtgtgta tgtgaggtgg tctactttcc
     6901 ccgcacgccg tcggtatcga cgacggggat cattgaggtg attagggggc atttctgata
     6961 atatgagttc ctctatttta ggtattttga tgaatattta aaaaatcata attcaaatga
     7021 atatagttaa tgtttatgag tttactgttc tctat
//
LOCUS       DAPQZD010000214         7047 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPQZD010000214 DAPQZD010000000
VERSION     DAPQZD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.216"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            120..1898
                     /locus_tag="RO454_004495"
     CDS             120..1898
                     /locus_tag="RO454_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDY0936220.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1937..2416)
                     /gene="xdhC"
                     /locus_tag="RO454_004496"
     CDS             complement(1937..2416)
                     /gene="xdhC"
                     /locus_tag="RO454_004496"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDY0936221.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2413..3291)
                     /gene="xdhB"
                     /locus_tag="RO454_004497"
     CDS             complement(2413..3291)
                     /gene="xdhB"
                     /locus_tag="RO454_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDY0936222.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3302..5599)
                     /gene="xdhA"
                     /locus_tag="RO454_004498"
     CDS             complement(3302..5599)
                     /gene="xdhA"
                     /locus_tag="RO454_004498"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDY0936223.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6013..6768
                     /locus_tag="RO454_004499"
     CDS             6013..6768
                     /locus_tag="RO454_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDY0936224.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6847..6920
                     /locus_tag="RO454_004500"
     tRNA            6847..6920
                     /locus_tag="RO454_004500"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6879..6881,aa:Gly,seq:ccc)
BASE COUNT         1764 a         1695 c         1782 g         1806 t
ORIGIN      
        1 agtgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
       61 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
      121 tggagcttgc gactacgcag tcagtattga tgcaaattca accaacaatt cagcgttttg
      181 ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa aacttgtgtc
      241 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggccg ccagttgagc ggcaactcac
      301 gtctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
      361 atcccctttg tgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
      421 cgccagtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
      481 agcaacaaga acatatcagt gataatttac gcgaattttc cgattacgtt cgccatatat
      541 ccaccatttt tgtttctaaa cttctggagg atcagggacc aggagataac atcagtaaaa
      601 tattcgcgac catgatcgat aatatggatc agggcgtgtt agtggttgat gctgataatc
      661 gggttcagtt tgttaatcag acagctttaa aaacactcgg tgttgtacaa aataatatta
      721 ttggaaaacc tgttcgtttc agaccattaa catttgagag taattttacc catggtcata
      781 tgcaacatat tgtttcgtgg gatgataaaa gtgaattaat cattggtcag ttgcataaca
      841 tccagggccg acaactattt ttaatggcgt ttcaccaatc gcataccagt ttttctgtag
      901 caaatgcacc tgatgaaccg catattgaac aattggttgg cgagtgccgt gttatgcggc
      961 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
     1021 aaagcggcac gggtaaagaa gtcgtcgccc gtgcaatcca taagttgagc ggaagacgga
     1081 ataaaccctt tattgctatc aactgtgccg cgattccgga acagcttctg gagagcgaac
     1141 tgttcggtta tgttaaaggc gcatttactg gcgcttctgc caacggtaaa acagggttga
     1201 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat tggtgatatg ccattaatgt
     1261 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
     1321 gcccaataca agtcgacatc cgcatcattt ctgcaactaa tcagaatttg gcccagttca
     1381 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
     1441 ttccaccgct gcgtgaacgt caggaagata ttgaattact agtgcattac tttttacatc
     1501 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcctgatgtc gtcgaaatat
     1561 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
     1621 ttaacgttgt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
     1681 ataatggcac aacggagcaa agtgatgtaa cagaggccag tgaggcgcac ctgtcactcg
     1741 atgatgcggg cggcacggcg ctggaagaga tggaaaagca aatgatccgc gaggcgcttt
     1801 cacgtcataa cagcaagaag gaagttgctg atgaactggg catcggcatt gctacgctct
     1861 atcgcaagat taagaaatat gagttgttaa acacataacc tctgagaatc cggcctgaat
     1921 tcaggccgga tatcttttac tttgttttct cgcaatccag aactgtattt acaatcatct
     1981 gataccccgt gcagcgacaa agatttcccg ccagtccacg acgaatttcc gtaatggtta
     2041 atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg cccggcgtac
     2101 aaaacccgca ttgcactgca ccagatttcg cataagccag ttggacatga gaaagtttac
     2161 cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag gccgcaagga
     2221 ataagcaact gtctatcgca gtgccgtcga ccagcaccgt acaggcacca cattcaccca
     2281 cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc gagagcggcg
     2341 tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg atggtaattg
     2401 tttcgctgtg attcattgca atttcccccc cgccgcggcg acggcttcgc taatcacttt
     2461 tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc atgacgaacg
     2521 cggggcgaca tcttgcagga ctgattcgct gatggcttcc agcgtttgca ggtttaatgg
     2581 cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc
     2641 aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc
     2701 aattgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg
     2761 ttctttcggc tgtggcggaa aatgaaaggc gacaaggatt tcgtcatgct caagagacac
     2821 tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc gcggggagtg
     2881 gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc tggtggcacc
     2941 gttgcaaata tttccgccgt aggtggcgac attacggatc tgcggcccgg cgattgatgc
     3001 agccgcagca cataacgccg ggagatgacg ttgagttaca ggatcttcaa tgagctgggt
     3061 aaatgtcgtt gcagagccga tacgtagcgc gccatcttcc gccagcgtaa ttccccgcag
     3121 ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat ggtgaagctg
     3181 tatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag tcagcaggtt
     3241 gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca tgatgttatc
     3301 ctcaaatcaa tcctgccaga tggaattctt catataatcg ttttggcgtt agcggcagtg
     3361 tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg gctacaggaa
     3421 ttatgggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca ttgatttcga
     3481 cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa tccagcagat
     3541 tggggttacg gaccacgccg cttttcgcat cgatgatcat ctcttcaaat agcgcccagc
     3601 caatgcccat ccccattccg ccgtgtacct gaccttctgc cagcagcgga ttaagaatat
     3661 ggcccgaatc atgaacgttg aggatgcggt tgatggttac tttacacagt gcaatatcga
     3721 ccgtcagatc aacaaaggta cagccaaacg ccggtgggtt agtggtggtt ttgatggagc
     3781 tttcggcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt
     3841 ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct tttatcaggg
     3901 tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt ttctctttta
     3961 ataacagtgc cgcactgcgc agcgcaggcg cggcaacata gctctgacgt gaggcaaatg
     4021 cgccggggtc gaatggtgta acgtcggtgt cttgtgtcga aattacgcgg acatcgctga
     4081 ccggaactcc cacggtttct gccaccattt gcgagaacac ggtgtcggca ccctggccga
     4141 tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaac aggcgcgcgc
     4201 ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa
     4261 cgccacgacg taaattgcct tgctggttct ggcactctgc acggcgtttt tcccattcaa
     4321 agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata cgttttcccg
     4381 tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa
     4441 tacctaacgc tgtcgcggca tcatcaagca tagactcaac ggcaaatacg acttgtggcg
     4501 cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc tttgaactgt
     4561 aagcgtaggc acaacgagga taaaggtaag cgactttatt ccccccagca gaagcaatgg
     4621 agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta taacctttca
     4681 atgttccgtc gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg cgggtacggg
     4741 ttgcgaggaa acactcttca cggctaagag aaactttcac cggaatgccg ccaagtttgc
     4801 ttgtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca aaaccgccac
     4861 cgataaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc tgaccaacca
     4921 cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc gagtcatcct
     4981 ccatccatgc cagcgatgtc acgctttcca tatgacaatg ttgaataacg ggcgtctgat
     5041 agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta cccgtcgaca
     5101 tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct tctgccagcg
     5161 ccgcttctga cgaggtgata acgggtaatt cttgccactc aatgctgacc aattgcaccg
     5221 ctttttctgc cgtgagttca tcgcgggcta cgacgatggc aacggcgtca ccatgatgac
     5281 gaacatgacg cgttagcagg gcacgatcgg cagtatcgcg cttgttttcg tcaagtgtcc
     5341 aggcatgccc tgccgtggcg aatgggattt ctggcacatc ttcccaggta aaaatcgcca
     5401 ggacgcccgg caaactcctg gcttgttcat cattaatact tacggcataa ccatgtgcga
     5461 tagggctacg tacatatttc gcgtaacaca tgcccgccat aacataatcg tcagtatatc
     5521 gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca cccgtagcgg
     5581 ttgcttcccg cgcttccata gaaatcccct cggtttgaac atttggttct tatggcaaag
     5641 atttagtctt agcaggcaag aattacgcca gaaacgacta tatgcgtgag gggaggaagg
     5701 aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg aatagggaca
     5761 gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattagga ttattacgca
     5821 tcaaggtggg aaatagggta aaagtcagga aattcgatac cactacagca aatattttgc
     5881 tgatgatact tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca
     5941 gaagcaagag attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag
     6001 cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg
     6061 ttatcggttg gactgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg
     6121 tattccggct ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc
     6181 acgggaacca gcgtaaaaga actggcgcga ctgaacggca tttccccccc ttacaccatt
     6241 gaagtaggtc agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca
     6301 accgccaaat ccacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct
     6361 tcctggccgc cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg
     6421 ccgtattcga cagcagatgg cggcaataaa gggattgata tctcagctcc acgaggtaca
     6481 cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac
     6541 ggtaatctca tcatgattaa acacagtgaa gattacatta cggcttacgc tcataatgac
     6601 acgatgctgg taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc cactatgggg
     6661 agcactgatg cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt
     6721 gatccgctac gttacttgcc gcctcagggc agcaagccaa aatgctgatg gcgaattaat
     6781 cagcagtcag cagcgtggta cttgctaagg agagcgtaag gtttataatg ccttacgcat
     6841 ctcgaagcgg gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc
     6901 gattcccttc gcccgctcca atttatcttc acatcatcta cctgccattt ttgttgtatc
     6961 tgattttcta tattatctta attcaagcgc ttcagacttt tcgcaatcgc cgtaatactt
     7021 ccccattttt ttgtaaataa aaagcgt
//
LOCUS       DAPQZD010000215         7037 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPQZD010000215 DAPQZD010000000
VERSION     DAPQZD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.217"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1140
                     /locus_tag="RO454_004501"
     CDS             <1..1140
                     /locus_tag="RO454_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDY0936225.1"
                     /translation="LSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQ
                     MSLCMLIFLALINKVSENTKQRNLFSKKVTLCISLFFVLGGPIVAHIFSSHYESYDLH
                     IAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNI
                     SLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNIFNRN
                     YFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPD
                     DLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVV
                     TQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(1245..1332)
                     /locus_tag="RO454_004502"
     tRNA            complement(1245..1332)
                     /locus_tag="RO454_004502"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1296..1298),aa:Ser,seq:gga)
     gene            1566..2504
                     /gene="ghrA"
                     /locus_tag="RO454_004503"
     CDS             1566..2504
                     /gene="ghrA"
                     /locus_tag="RO454_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDY0936226.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2559..3296
                     /gene="ycdX"
                     /locus_tag="RO454_004504"
     CDS             2559..3296
                     /gene="ycdX"
                     /locus_tag="RO454_004504"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDY0936227.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3320..3874
                     /gene="ycdY"
                     /locus_tag="RO454_004505"
     CDS             3320..3874
                     /gene="ycdY"
                     /locus_tag="RO454_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDY0936228.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3976..4467
                     /locus_tag="RO454_004506"
     CDS             3976..4467
                     /locus_tag="RO454_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDY0936229.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(4532..5365)
                     /gene="csgG"
                     /locus_tag="RO454_004507"
     CDS             complement(4532..5365)
                     /gene="csgG"
                     /locus_tag="RO454_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDY0936230.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5392..5808)
                     /gene="csgF"
                     /locus_tag="RO454_004508"
     CDS             complement(5392..5808)
                     /gene="csgF"
                     /locus_tag="RO454_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDY0936231.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5833..6222)
                     /gene="csgE"
                     /locus_tag="RO454_004509"
     CDS             complement(5833..6222)
                     /gene="csgE"
                     /locus_tag="RO454_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDY0936232.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6227..6877)
                     /gene="csgD"
                     /locus_tag="RO454_004510"
     CDS             complement(6227..6877)
                     /gene="csgD"
                     /locus_tag="RO454_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDY0936233.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1825 a         1552 c         1699 g         1961 t
ORIGIN      
        1 cttagtttgg cttttctaag ttgtttgatt tactttgtta aaaccgtcat tatcattcag
       61 caaattattg aggggcgttt gacaagcagt gttgttcaaa atgacattgc aatttattat
      121 ttgtttcgtc agatgagttt gtgcatgtta atatttctgg cattgataaa taaagttagt
      181 gaaaacacaa aacagcgcaa tttattttcc aaaaaagtga ctttgtgcat aagtttgttt
      241 tttgttcttg gggggccgat agttgctcac atattttcta gccattatga gagctacgat
      301 ttacacattg ccgagttaac caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt
      361 accataatga ttttcatgtg gttaaccttg ctttcggtta atctatactt taatggttta
      421 cgctgtgata tctggaacgg ggtgtcggtg attgcttttt gtgccgtgct gtacaatatc
      481 tctctgttat ttatgagcag gtatagcgtg tccatttggt atattagtcg cactattgaa
      541 gtagtcagta agttaaccgt tttggtaatt tttatgtgcc atatattcag tgcgctacga
      601 gtaacaaagg acattgcaca tcgcgatccc ttaaccaata tattcaacag aaattatttt
      661 tttaatgaac tgacggttca atcagcatcg gccaaaaaaa caccttattg cgtcatgatt
      721 atggatattg accacttcaa aaaagtcaac gacacctggg gacatccggt tggcgatcaa
      781 gtgataaaga cagtggtgaa tatcattggg aaaagcatac gaccagatga ccttttagcg
      841 cgcgttggcg gcgaagagtt tggcgtcttg ctgacggaga tcgatactga atgtgcgaaa
      901 gctttagcgg aaaggattcg ggaaaatgtt gaacgtttaa ctggcgataa tcctgaatac
      961 gccataccac aaaaagtgac gattagtatt ggcgctgttg taacgcagaa aaatgagtta
     1021 aaccctaagg agatctatca actggctgat aacgcacttt acgaggcgaa agagaccggg
     1081 cgtaataagg tggtagtaaa agaggcagaa attcttatca accgcaagga tgatgaatga
     1141 gtctgtcgct agttgtcaac ctccgcctgg ggcttctcat ccccccggtg tatgcaatat
     1201 acgaaaaaaa agctcgtact ttcgtacgag ctcttcttta aatatggcgg tgaggggggg
     1261 attcgaaccc ccgatacgtt gccgtataca cactttccag gcgtgctcct tcagccactc
     1321 ggacacctca ccaaattgtc gttcctgtct tgctggaacg ggcgctaatt tagggaaatc
     1381 atgacctgag gtcaacaaac ttttttaaaa aatcgctcga ttattcaaac ttcaatcaat
     1441 atgtagtttt aataagcgaa atttgctttt tttgtcaccg accacggatt tgttatgctg
     1501 gtggcctttg tagatcataa cgataagtgc gaataaattt cgcgcaacgc ttttcgggag
     1561 tcagtatgga tatcatcttt tatcacccaa cgttcgatac ccaatggtgg attgaggcac
     1621 tgcacaaagc tattcctcag gcaagagtca gagcatggaa aagcggagac aatgaatcag
     1681 ccgattatgc tttagtctgg catcctcctg ttgaaatgct ggcagggcgc gatcttaaag
     1741 cggtgtttgc acttggggca ggtgttgatt ctattttgag caagctacag gctcaccctg
     1801 aaatgctgaa gccttctgtt ccactttttc gcctggaaga taccggtatg ggcgagcaaa
     1861 tgcaggaata tgctgtcagt caggtgctgc attggtttcg acgttttgac gattaccgcc
     1921 tccagcaaaa tagttcgcat tggcaaccgc tgcctgaata tcatcgggaa gattttacca
     1981 tcggcatttt gggcgcaggc gtactgggca gtaaagttgc acagagtctg caaacctggc
     2041 gcttcccgct gcgttgctgg agtcgaaccc gtaaatcgtg gcctggcgtg caaagctttg
     2101 ccggacggga agaactttct gcatttctga gccaatgccg ggtattgatt aatttgttac
     2161 cgaatacccc tgaaaccgtc ggcattatta atcaacaatt actcgaaaaa ttaccggatg
     2221 gcgcgtatct cctcaacctg gcgcgtggtg ttcatgtcgt ggaagatgac ctgctcgcgg
     2281 cgctggatag cggcaaagtt aaaggcgcaa tgctggatgt ttttaatcgt gaacccttac
     2341 cgccggaaag tccgctctgg caacatccac gcgtgacgat aacaccacat gtcgccgcga
     2401 ttacccgtcc cgctgaagct gtggattaca tttcgcgcac cattgcccag ctcgaaaaag
     2461 gggagagggt ctgcgggcaa gtcgaccgcg cacgcggcta ctaataaagc atcaggtttc
     2521 ctgctatcct tggcgggaat tgaatacagg agagagttat gtatcccgtc gaccttcata
     2581 tgcataccgt tgccagcacg catgcatata gcacattgag tgattacatt gcccaggcca
     2641 aacaaaaggg cattaaactt tttgcgatca ccgatcatgg cccggatatg gaagatgcgc
     2701 cgcatcactg gcacttcatt aacatgcgta tctggccgcg agtggttgat ggggtaggga
     2761 tcctgcgcgg catcgaagct aacattaaaa atgttgatgg tgaaattgat tgcagcggca
     2821 aaatgtttga ttcgctggat ctgattattg ccggttttca tgagccggtt tttgcgccac
     2881 atgacaaagc aaccaataca caagcgatga tcgccactat cgccagcggc aatgtgcata
     2941 taataagcca tcccggaaat ccgaaatatg agatagatgt taaagccgtt gctgaagcag
     3001 ccgcgaaata tcaggtggcg ctggaaatca ataattcctc atttttacac tcacgtaagg
     3061 gcagtgaaga caactgtcgt gcggtagctg cagcggtacg tgacgctgga ggttgggtgg
     3121 cattaggctc ggattctcac actgcattta ccatggggga atttgaagag tgtcttaaaa
     3181 tcctcgacgc ggtagatttt ccgccagaac gcattttgaa tgtttctccg cgccgcttac
     3241 tgaacttcct tgaatctcgc ggtatggcac cgattgcgga atttgcagac ctttaattac
     3301 tagtaatgga aatatattaa tgaacgagtt ttctatcctc tgtcgtgtgc tgggttcgct
     3361 ctattaccgc caaccgcaag atcctttact ggtgccgttg tttaccctga ttcgtgaggg
     3421 gaaactggct gcgaactggc cactggagca ggatgagtta ctgacgcgtt tacagaaaag
     3481 ctgtgatatg acgcaagtct ctgccgatta caatgcgttg tttatcggcg atgaatgtgc
     3541 tgtgccgcca tatcgtagcg catgggttga gggcgcaacg gaagcggaag tgcgcgcttt
     3601 tctttccgaa cgagggatgc cactagcgga tacgccagcc gatcacatcg gcacattgct
     3661 gcttgcggct tcctggctgg aagatcagtc aacggaagat gagagcgaag cactggaaac
     3721 actgttcagt gagtatctgt taccctggtg tggtgcgttc cttggcaaag tggaggccca
     3781 tgcaactacg cctttctggc gcaccatggc accgctaacc cgcgatgcta ttagtgcaat
     3841 gtgggacgag ctggaagaag attctgaaga gtaaatgtga tctgcatcac tcataactgc
     3901 aactgataat ttgttgttgc ataaaatgtg tgctcgatct cattcatggc cgcgttttct
     3961 gctatcatgc gcggcatgaa catacttctc tctattgcaa tcacaacagg cattctctcc
     4021 ggtatctggg gatgggtggc tgtttccctg ggcttactaa gctgggcggg cttcctgggc
     4081 tgtacggcct actttgcctg cccgcaaggt gggctgaaag ggctggcgat ctccgctgca
     4141 accctgctta gtggcgtggt gtgggcgatg gtcattattt acggtagtgc actggcacca
     4201 catctggaaa ttctcggtta tgtaataacc ggtattgtcg cgtttctgat gtgtattcag
     4261 gccaaacagc tgctgctttc atttgttccg gggacgttta taggcgcatg cgcgacgttt
     4321 gccgggcagg gggactggaa actggtgtta ccttcgctgg cgcttggact ggtatttggt
     4381 tacgcaatga aaaacagcgg cctgtggctg gcggcgcgta gtacgaagac cgctcaccgt
     4441 gagcagcaaa tcaaaaataa agcgtgaggg gcactcacgc tttcgcttaa acagtaaaat
     4501 gccggattat taattccggc gtttttatct atcaggattc cggtggaacc gacatatggc
     4561 ggtatttcac cagaatgtca ttctgccgtt ctgttttatt ttgcaaatcc caaaggccgc
     4621 ggtcgatacc atcattaatc aggaaaatga cgcctgtttc gatagccgac atcaggcaca
     4681 gcataacggg ttcgttcgag gtgtaaccta cttccccttc cagcaagcgc tggtagtcaa
     4741 taaagcggaa gacccccgcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg
     4801 aggagaggat ctcaccggta ctcacattga cgacacgcag gttcacggca atctgatcaa
     4861 gctggtattg cgtgtctgca ccgatcccaa aatatcttgc cccaacaccg cccgatttaa
     4921 cgttgctttc ataaccgata atcgaacctt caaccatgat atttgccgcc gttaaagatt
     4981 gcagcgggat tcggttatta atggcaactg tgccgttttc ttgtgccgca cgaataatct
     5041 tgcgctcgtt aagcagattt tgtaagccct ggcgctccag cggtataaac cagcgagaat
     5101 ctttcagtgc cgtgaccagc attgccgtgg cgctttgcgg aacagcagtg gagaagttac
     5161 ttgccgggta gggtttaaat tgtccggttt cgtcctgaat gttgtatacc gaaacaaaga
     5221 tcttacccgt cggcgctggc agatgggtca aatctttgta gctctgagca cgaggcatta
     5281 atgtcggtct ggcggcttct ttaggcgggg cggttaagca tccgctcagt aacatgacgg
     5341 caaccaaaag aaataagcgc tgcatgatta ttttccttat gaagctgggg cttaaaaatc
     5401 ggttgagtta ttttgtaaac ccgaaacctg gatggtcgag gtttgtccgg ttttacgatc
     5461 tgtcacgttc aactgcaatt gaccatcgcg gttagcaata tcaacaatat aatcgttggt
     5521 caccatgcgg cccggtttac cggtattaat attcgacagt agcccaccca aaatttgtga
     5581 ctggatggcc tgagtaaagt tatctaacgc tgatggcgtt tcaataccaa agtcatcgtt
     5641 atagctcgga tctttataag agttttgtgc ctgagcgcta tttaacaaaa aagcgccatt
     5701 atttgggtta ccaccaaagt ttggattacg gaactggaaa gtcatggatc cagcccaact
     5761 taatggcgaa ataagcatga gtagaactac tgcatgtttg acacgcattg cagcctccga
     5821 acaatttatt atttaaaatt catcatgcgc caaatcgccc gtacttaata acgcctggtt
     5881 tatctggcga cgatttagtg cttcttcagt ttgaatcagt gcaaagacga cagttttctc
     5941 gaagtctctt ttcaacggaa ataaaaaagt ctggaaaata acgtcctgat tgaccgttat
     6001 agtgatccaa cttccccatc gtgcactggg cctttcatta atcgttaagt tacccgtata
     6061 gtcactttcc catttatcac taaaggctcg gtaaaaatca tggccaatag atgaaacagt
     6121 atggtcagtt aacaatcccg ggacttctac ctcaacggca tgaagattcc ctgcggcgaa
     6181 cagaaattct gccgccacaa tccagcgtaa ataacgtttc atggctttat cgcctgaggt
     6241 tatcgtttgc ccaggaaacc gcttgtgtcc ggttttttac ggctatcttc ttgaaaagat
     6301 tataaagatg cgttttaacc gtattttcgc tgatgaacaa cgaacgagcg atctcgttat
     6361 tagacgcgcc gatacgcagc ttattcagga tctctttttc ccgatgagta aggagggctg
     6421 attccgtgct gttataacga tagttacctg aatgcgtaat caggtaactg gcaagctttt
     6481 gcgtaaagta gcattcgccg cgcaggacgc cttgcaaccc attgacaaca cgttcttgat
     6541 cctccatggc ataaaaaacg ccgttgatat gaggccagtt ttcaatgtcg cggtacgggt
     6601 aatcttcagg cgtatttagc aacaatattt tgatattgtt gtttttcctg ctcaaagtat
     6661 cctgccaata atggataagc tttttatccg cttccatcat atccagaaga ataatagagc
     6721 ctgaagatat atcgtccaga gaacgttgaa tattatgtaa ttttcctgta atcgccagcg
     6781 attgtttaag gtgctgcaag agagctgtcg cttgcaaaga aggtttagtg atcaacaata
     6841 atgtatgacc atgaatacta tggacttcat taaacatgat gaaaccccgc ttttttattg
     6901 ttcgcacacc tgacagctgc ctctaaaata gaagcaccag aagtactgac agatgttgca
     6961 ctgctgtgtg tagtaataaa tcagccctaa atgggtaaaa tataaaacta atggattaca
     7021 tctgatttca atctagc
//
LOCUS       DAPQZD010000216         7024 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPQZD010000216 DAPQZD010000000
VERSION     DAPQZD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.218"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            7..963
                     /locus_tag="RO454_004511"
     CDS             7..963
                     /locus_tag="RO454_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HDY0936234.1"
                     /translation="MTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNL
                     GHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELNSL
                     SDKIQKLSDDAATANADPADMAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQL
                     AAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIRLIANKGAVSVQAQHDLME
                     LLAKKSIEIVSTEDEIKITAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYGRQ
                     PKASEKVPMPEFPILSAVDSSDFCLECLLNAIKNDDAVVEGV"
     gene            966..1787
                     /locus_tag="RO454_004512"
     CDS             966..1787
                     /locus_tag="RO454_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HDY0936235.1"
                     /translation="MSSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYTPRVLKQLAARPDMDWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTVANNSDETVEIITLDEPLWQKIADRGDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQVVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            1831..2358
                     /locus_tag="RO454_004513"
     CDS             1831..2358
                     /locus_tag="RO454_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HDY0936236.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAIPVAYFLLCVGIS
                     IWGAPTGGVILVIHSELDRPILGFSVNGMAGANAFAHGGGSTTCCGDIRGKEAEVVWT
                     LSTTRAQYNTGLREEVRRITLPLPERKHGQDFLHVHFLPGDKVFLGWSEGAGSPYEKR
                     KEPSYPSRKNQEAQP"
     gene            2355..4223
                     /locus_tag="RO454_004514"
     CDS             2355..4223
                     /locus_tag="RO454_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HDY0936237.1"
                     /translation="MISESVISAAVRAQQSEDGKVGNCGRIWHVAFFFDGVGRNIDRD
                     ATDCRLSNIARLFRAYPDEQKNTGDTCYNKFYFSGMGTAYHDDSVDNIHALMDIGLDN
                     FLEDMKSLPEDTISDAGESVIKGEKKWTDVLGNLLEDLNNPVEWVKGIGKMAAKSVGK
                     AGIESTPWLRDSEAMSAYFMTGEPVRLEAAKRLFKKFYTENMKGDVPIKKISVSLYGF
                     DLGATLARKFLDNFLKEVCLKNKEKKHQYKNALVDIAFIGLFDCSRHSPTSSNNGLDY
                     FFMWLGLPGKMIGSVLGEKAIDQDSPLPDVVNNALHLVAAHERRPWRGLYLLGKKTDT
                     ANKKYREELLPGCSEDIGGGLKPDEQKPSAELCRVALYNMYHAACQAGVPFPDFDTLD
                     QYDTKVASYFIMNDAVQGGSVNYWVSRYQREEVKDKVFSEAMQEKYLDNYFLWLGEQY
                     YMYQYERERLDKELTSAHRGKISDYGPLAGTGINPNTNADDIKSQISELDSLWGWLND
                     VKSVAIGLRNDFIVNPRKNDSRIKLKPQVYNAAVNRAVKFLEFSRAVYNDKNLPSSSS
                     LTANTLYSFFVHDIQKVDHTPSISEDFFLRRSSEIPDADEPPGNKDDGKKHTRRDD"
     gene            4329..6842
                     /gene="vgrG"
                     /locus_tag="RO454_004515"
     CDS             4329..6842
                     /gene="vgrG"
                     /locus_tag="RO454_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDY0936238.1"
                     /translation="MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIE
                     FTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQV
                     MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQGKVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGV
                     AIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGL
                     KMSGITDENGNTELISSDKEEVVNISVFEPDEFLDDEIN"
     gene            6864..>7024
                     /locus_tag="RO454_004516"
     CDS             6864..>7024
                     /locus_tag="RO454_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936239.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVR"
BASE COUNT         1986 a         1529 c         1907 g         1602 t
ORIGIN      
        1 gaccatgtga ccattcagaa ctacaagcgc aacgtgctac gcaccccggc gaacaacaaa
       61 atccgcctcg acgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc
      121 aagagccagc tcaacctcgg tcatctggtg gatgccggga aacagcagcg cggggaaggt
      181 tttgaactgc gtaccgatct gtggggtgca gtacgggcaa agaaaggcat ctttatcagc
      241 gccgacgcgc aggacaaggc gcagggtcag gtgcgcgaaa tggccgacat tatcagcgaa
      301 ctgaacagcc tgagcgataa aatccagaaa ctgtctgacg atgcggccac ggcgaacgcc
      361 gatccggcgg atatggccgc gcagatagca ttgataacca gccggataaa tgacctgacc
      421 gcctcggtga ttttgatgca cgcaccgaaa ggcgtggcgg tcgccagcgg cgagcacctg
      481 caactggccg cggtgaaaaa cctccagata aatgcgggaa acaacgccga tatcggcgta
      541 gtgaagaata tgtttatcgg cgtgggacgg gcgctgagcg tgtttgtacg caaggcgggt
      601 atcaggctga ttgccaataa aggcgcagta tcggtacagg cgcagcacga tctgatggag
      661 ttactggcga aaaaatcgat agagatcgtc agtaccgaag atgagattaa aatcaccgcg
      721 aagaaaaaaa taacgattaa tggtggtggg agttatatcc ggatagaggg tagcggcatt
      781 gagccgggaa cgccggggga ttataacgtc aaagcggtac attacgggcg tcagcctaaa
      841 gcctcggaga aggttcctat gccagaattt cccatccttt cagccgtgga ctcatccgat
      901 ttttgtcttg aatgcctgct gaatgccatc aaaaacgatg acgctgttgt tgaaggagtg
      961 taatcatgtc ttcagaacaa caactggcag accactggct taagcgaaac gaaacgttaa
     1021 acgcctttat tttatttgaa ggtggagcgc tctctcagga aatgcttcag atcatgatgg
     1081 agcattataa ataccgacga atttccctgt atcaactgcc cgatcttaaa aaggtagcag
     1141 cttacggtcc ctggttggtt cgtattgaaa gtgaaaagga tctggcgatg gtgcttgtcg
     1201 ggaaactacc tgtcgcaggc attactttct ccgaattgca gctggagcca ctggcgtctc
     1261 ggcttgctgt gggatgtatg gcaaaaacgc cggatggtcg tgaagttctg ctgaggtact
     1321 acacgccccg agtgctgaaa caactggctg cccggcctga tatggactgg catccggtaa
     1381 tgttttctca gatagaatca tggtggatgc tggaagatac gcggtggcaa aggctgaaca
     1441 tgccgcttac tgtggcaaat aacagcgatg agacagtgga aatcataacg cttgatgaac
     1501 cgctctggca gaaaatagcg gatcggggag atgtatctgc gcttttaaga gagtggcgca
     1561 ccatgaaaac gagtgagcac ttcccgccct gtattcagcg aaatatggtg gagaaggcct
     1621 tgaataaggc tcaggtggtc ggattgacaa agccactgga tcagaaaatt tatgcgttga
     1681 gctatctgaa tggaaagaaa gcgtatcttg agtcagccga atttcaggtc atgctggaaa
     1741 aggtgaaacg aaatgaaata tcgctgtctg accttggtcg ccagtgacat acatctttgc
     1801 caaccaattg ataaaaggga ataaaatctg atggggctat ttacccgact ggaaaaattt
     1861 gaccaaaagc ttacgcgtgg ttatgcccgc tgggggcgtt gggtgtggcg gatgctaata
     1921 gcgatccccg tggcttattt tttactgtgt gtaggaatat caatctgggg agctccaact
     1981 ggaggagtca tattggtgat ccatagtgaa ctggataggc caatattagg attcagcgtg
     2041 aacggcatgg ctggggcaaa tgcgtttgca catggtggag gttcaacgac ttgctgcggt
     2101 gacatcagag ggaaagaagc tgaggtcgtc tggacattga gtacgactcg cgctcagtat
     2161 aatacgggac tgcgtgaaga agttcgacgt atcactctgc ctttgcctga aaggaaacat
     2221 gggcaggatt ttttgcatgt acatttcttg cctggtgata aggttttttt gggttggagt
     2281 gaaggagctg gttcgcccta tgagaaacga aaagaacctt cttatccgag tcgaaaaaat
     2341 caggaggcgc agccgtgata agtgaatcag tgatttccgc agctgtacga gcacaacagt
     2401 cagaagatgg taaagtggga aattgtggcc ggatctggca tgtcgctttc ttttttgatg
     2461 gagtgggacg aaatatcgac cgggatgcga cagactgccg tttgagtaat attgcccgcc
     2521 tgttccgggc atatcctgat gaacaaaaaa atacaggtga tacttgttat aataaatttt
     2581 atttctccgg aatgggcact gcctaccatg acgattctgt cgacaatatt cacgctctta
     2641 tggatattgg tttagataac tttctggagg atatgaaaag tctgccagag gatactatat
     2701 cggatgcagg ggagtctgtt attaaaggag aaaagaaatg gacggatgtg cttggtaatc
     2761 tccttgagga tctcaataat ccggtagagt gggttaaagg gattggcaag atggcggcta
     2821 agtcggtagg caaagcagga attgagtcca caccttggtt gcgtgatagc gaagccatga
     2881 gcgcctattt tatgacggga gaacccgtac gccttgaagc tgcaaagcga ttgtttaaga
     2941 agttttatac ggaaaatatg aagggagatg ttcccatcaa aaaaatttct gtttcattat
     3001 acggttttga ccttggagct acactggcac gtaaattttt ggataatttt cttaaggaag
     3061 tttgcctgaa aaataaagaa aaaaaacacc aatataaaaa cgcgttggta gatattgctt
     3121 ttattggtct ttttgactgt tctcggcatt caccgactag tagtaataat gggttggact
     3181 atttttttat gtggttgggt ttaccaggga aaatgattgg ttcagtatta ggtgaaaaag
     3241 ctattgatca ggactcacca ctgccggacg tagtaaataa cgcacttcac cttgtagcag
     3301 ctcatgaacg gcgtccatgg cgtggtctgt atctgttggg gaaaaaaacg gatacagcga
     3361 ataaaaaata cagggaagaa ctgcttcccg gttgtagtga agatattggt ggtggcttga
     3421 aaccggatga acagaaaccc agtgcagaac tgtgcagggt tgcactatac aatatgtatc
     3481 atgcagcatg tcaagctggt gtgccatttc ctgattttga cactttggat caatatgata
     3541 cgaaagttgc cagctatttt attatgaatg atgctgttca gggaggatct gtaaattact
     3601 gggttagtcg ttatcaaaga gaggaagtga aggataaagt atttagtgag gcaatgcagg
     3661 agaagtatct agataactat tttctctggt tgggtgagca atattatatg tatcagtatg
     3721 aacgggaacg tctggataag gagttgacat cggctcatcg tgggaaaata agtgactatg
     3781 ggccacttgc aggtacggga attaatccaa atactaacgc tgatgatata aaatcgcaga
     3841 tttcagaact tgactcacta tggggatggt tgaatgatgt aaaaagtgta gctattggat
     3901 tacgaaatga ctttatagtc aacccgagga aaaatgatag ccgaataaaa cttaaacctc
     3961 aggtttataa tgctgctgtc aatcgggctg tgaaattcct tgagttttct cgcgcagttt
     4021 ataatgataa aaatctgcca tcatcatcat ctttgacggc aaatacacta tattcctttt
     4081 ttgtgcatga tatacaaaag gttgatcaca caccttctat atctgaagat ttcttcttac
     4141 gtcgctcatc tgaaattcca gatgcggatg aacctccagg aaataaagat gatggaaaga
     4201 aacatactcg cagggatgat tagaaattat aagtagctta ctttaattag ttatattttt
     4261 tgcatttttc cctttttaaa gagaatcata cttggattta tttttcataa atataaagga
     4321 gtacagagat ggatatcccg actctgaatt tcgaccactc acaccacaag ctcaaaattc
     4381 gcggcctgaa aagcccggcg gatgtgctga cgtttacggg ccacgaacag ctcagctcac
     4441 ccttccgcta cgacattgag tttaccagta ccaataaagc aataacgccg gagtcggtgc
     4501 tgatgcagga cggcgcattc agcctgacgg caccgcccgt gcagggaatg ccggtgcaga
     4561 ctgccctgcg caccctgcac ggcgtcatta ccagctttaa acacctctca tcgtcacagg
     4621 acgaagcccg ttacgaagtg cgccttgaac cccgtatggc gctgcttacc cgcagccgcc
     4681 agaacgccat ctaccagaac cagacggtgc cgcaaatcgt cgaaaaaatc ctgcgcgagc
     4741 gccatcaaat gcgcggtcag gacttcgtgt ttaacctcaa aaacgaatac ccggcccgtg
     4801 aacaggtgat gcagtacggt gaggacgacc tgacgtttgt ctcacgcctg ctgtcggagg
     4861 tgggtatctg gttccgcttt gccaccgacg cccggctgaa aatcgaagtt gtcgagtttt
     4921 atgacgacca gagcggctat gagcgcggac tgacgctgcc gcttcgtcat ccttcagggc
     4981 tgtttgatgg tgaaacggaa gccgtatggg ggttgaacag cgcgtacagc gtggtggaaa
     5041 aaagcgtctc cacgcgggac tacaactacc gtgaagccac ggcggaaatg accacaggcc
     5101 agcatgacgc caccgggggc gataacacca cctacggcga agcctaccac tacgctgaca
     5161 acttcctgca aaaaggcgat aaagaggcag cggaaagcgg cgcgttttat gcccgtatcc
     5221 gccacgaacg ctatttgaat gaacaggcca ttctacaggg ccagagcacc agttcactgc
     5281 tgatgccggg gctggagata aaagttcagg gtgacgacgc gccagcggtg ttccgcaagg
     5341 gcgtactgat taccggcgtg acggccagcg cggcgcgtga ccgcagctat gaactgacgt
     5401 ttaccgccat cccgtactca gaacgctatg gctaccgccc ggcgcttatc ccctgccccg
     5461 tgatggccgg cacgctcccg gcacgggtca ccagcacggt gaagaacgac atctacgccc
     5521 atatcgacaa ggacgggcgc taccgggtca acctcgactt tgaccgcgat acgtggaaac
     5581 cgggttatga aagcctgtgg gtacgccagt cgcgcccgta tgcgggagac acctacggcc
     5641 tgcatctgcc gctgctggcg ggaacggaag tcagcatcgc gtttgaggaa ggcaacccgg
     5701 accgacctta tattgcaggc gtaaaacacg actcggcaca caccgaccat gtgaccattc
     5761 agaactacaa gcgcaacgta ctgcgcaccc cggcgaacaa caaaatccgc ctcgatgacg
     5821 agcgcggcaa agagcatatc aaggtcagta ccgagtatgg cggcaagagc cagctcaacc
     5881 tcggccatct ggtggatgcc ggaaaacagc agcgcgggga aggctttgaa ctgcgtaccg
     5941 atctgtgggg tacagtacgg gcaaagaaag gcatctttat cagctccgac gcgcaggaca
     6001 aggcgcaggg taaggtgcgc gaaatggctc cggcgatggc aatactggat ggcgcacagt
     6061 cacagatgaa gtcgctgtcc actgatgcgc agacggcgaa tgccgatccg gcagatctgt
     6121 catcacagat agcgctgttg cagcagagtg taaaagatct gacgcaggct gcgatcctgc
     6181 taagcgcacc caaaggcgtg gcgatcgcca gcggagagca cctgcaactg gcggcgtcga
     6241 aaaacctgat cgcgaacgca ggaaatcacg ctgatatcgg tgtcgtgaag aatatgttta
     6301 tcggcgtggg tcaggcgctg agcgtgtttg tacgcaaagc gggtatcaaa ctgtttgcca
     6361 ataaaggggc gatctcggta caggcacaga acgatctgat ggaactgttg gcgcagaaat
     6421 cgatagagat caccagtacg gaagatgaga ttaaaatcac cgcgaagaag aaaatcaccc
     6481 ttaacggcgg tggcagctac atccggctgg atgcctgtgg gatagaggcc ggtacgccgg
     6541 gagaatataa cgttaaggcg ggatattacg ggcgtaagcc gaaggcgaag ctgacgccgg
     6601 agctgatggc atttccggtg attaagtctg aggattttaa tcagagcttt attttgctgg
     6661 atgaaaatac tgggcaacca cttattaatt ggccctatga actagaactg gaaagtgggc
     6721 taaaaatgag tgggattact gatgaaaatg gaaatacaga actaatatcc tctgataagg
     6781 aggaggtcgt taatattagt gtgtttgaac ccgatgagtt tttagacgat gaaattaatt
     6841 aagacatata gcgagaatat aacatggcag ataaatccga aaagaaaaaa gaaaccacaa
     6901 ataaatcagc aaaaaaaaca ctaacgaaga agagcgaaaa attaactcca gcaaaagaca
     6961 ccgcaccaaa acaagtgaat gtagatccag ctataagatt gaaacaaatt cgtcaacttg
     7021 tccg
//
LOCUS       DAPQZD010000217         6978 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPQZD010000217 DAPQZD010000000
VERSION     DAPQZD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.219"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..512
                     /gene="smrB"
                     /locus_tag="RO454_004517"
     CDS             <1..512
                     /gene="smrB"
                     /locus_tag="RO454_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDY0936240.1"
                     /translation="FRQLMAGTRKIKQDTIVHRPQRKKISEVPVKRLIQEQADASHYF
                     SDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHGLTQLQAKQELGALI
                     AACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPKEYGGDAALLVLIEV
                     EEWLPPELP"
     gene            complement(572..1396)
                     /locus_tag="RO454_004518"
     CDS             complement(572..1396)
                     /locus_tag="RO454_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDY0936241.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(1398..1925)
                     /locus_tag="RO454_004519"
     CDS             complement(1398..1925)
                     /locus_tag="RO454_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936242.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDAASTAQGVGIGMEKTDGTAVLINNTSGATFVLSSGNNDLHFNAWLQAKSGRDVT
                     MGDFTASLTATFEYI"
     gene            complement(1922..2401)
                     /locus_tag="RO454_004520"
     CDS             complement(1922..2401)
                     /locus_tag="RO454_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936243.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINLSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(2398..2889)
                     /locus_tag="RO454_004521"
     CDS             complement(2398..2889)
                     /locus_tag="RO454_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936244.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGTTTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(2906..3658)
                     /locus_tag="RO454_004522"
     CDS             complement(2906..3658)
                     /locus_tag="RO454_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDY0936245.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(3678..6326)
                     /locus_tag="RO454_004523"
     CDS             complement(3678..6326)
                     /locus_tag="RO454_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0936246.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQSLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGISGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDEDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDASQSDMARTGNDKE
                     MYTITYNQNFAAAGVSVYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNTDKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQINVG
                     TSEQHGSVDGYLSHDGTLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGINAGEHMMVFWEGSAQCEIVLPKPLPADLFRGLLLPCEQKGTTAPDSLAP
                     EIKPVIQDQTRQVTPSEAPTSISATQ"
     gene            complement(6407..6973)
                     /locus_tag="RO454_004524"
     CDS             complement(6407..6973)
                     /locus_tag="RO454_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936247.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT         1531 a         1902 c         1767 g         1778 t
ORIGIN      
        1 tgtttcgcca gttgatggcg ggtactcgca agattaagca ggacacgatt gtccaccgac
       61 cgcagcgtaa aaaaatcagc gaagtaccag taaagcggtt gattcaggag caggccgatg
      121 ccagccacta tttttcggac gagtttcaac cattattaaa tactgaaggt ccggtgaaat
      181 atgttcgccc ggatgtcagc cattttgagg cgaagaagtt acgccgtggc gattattcac
      241 cggagttgtt tcttgatttg cacggtctga cgcagctaca agccaaacag gaactggggg
      301 cgttgattgc cgcctgccgc cgtgaacatg tgttttgtgc ctgcgtgatg catgggcacg
      361 ggaagcatat tttgaagcag caaacgccgc tgtggctggc gcaacatcca catgtgatgg
      421 catttcacca ggcaccaaaa gagtatggtg gtgatgcggc tttgttggtg ttgattgaag
      481 tggaagagtg gctgccgccg gaattgccct aataaatatt gcggtataaa cgtcttctcc
      541 gaccaggata ctgaccggag aagaattaac cctacaactc attcaaaatg tactggaaac
      601 gacaggtggc atcgattggc tgctgactgc tggtgtcgat ccgtatttgc atatcatgca
      661 gttgcttatc ggtaattgga tgtgctaccg gcgttactgt gactttgctg tcgtcacacc
      721 ccgacgtgct gaatgtgatc atatacgaca taaagcgaca ataagcgcca cgatcgtcca
      781 ttcggcttcc atcacacacc cgcccgtcgc gcatcaggta agtcgagagc gtttgtgtta
      841 tgactccccc cgtcgattgc agcgttagca tcttgttatg gaggacgtag ttctcattcc
      901 aggagatatc ataggggcca accggctccg ttgggcaccg ggtttgtttt gctgatggac
      961 caatgtaagt ataacctctc gcagcagtac tcaccgtgtt ggtactgacc aaccacgggc
     1021 acccgtgagt ttcaccatgc acgatgatat cgacccccag atcaacacat gaccatccat
     1081 ccgtaatccc tgggcaattg gagttatcca cagtattact ctgatgagtg atgttcatcg
     1141 ttggtgctga tcccggtccc gtccaaaccg tgttagggat attccccaac cacaacgtat
     1201 atccatcctg tgcatattcg ccataataga ccccttccgg cgtcaagaca tcgatacgaa
     1261 agacaacttt tgttgtagcc ccgttacccc aaaatccggt ataagtcttc acatccctga
     1321 atgtgacgtc cgcccaggcg gttgcactca gtagcccggt cagcagtaaa aaaaacagtc
     1381 cgctcatttt tttcatgtta aatatactca aacgtcgcag tgagggaggc cgtaaagtca
     1441 cccatcgtca catcccgccc gctttttgcc tgtagccagg cattaaaatg cagatcattg
     1501 ttgccgctgc tcagcacaaa tgtcgccccg ctggtattgt tgattagcac agcagtgccg
     1561 tcggtctttt ccatgccaat ccccacacct tgtgctgtcg aagcggcatc cagcgccaga
     1621 aaaccgggct gctcgctgtc ttccgttccg gtaagtgtca cccgaacctc atactgagca
     1681 ggccctttgc aatctttaag ctgaaacacc accggtacgc gcgcagattg cccggtcacc
     1741 atcagatctc tacggctgac cgtggggaaa tgcacttccg ccagcacctc gccctgaacc
     1801 accagcgtgc aggacttact gagtaaattg ccgtagaaat gcaggttgtc tgccgcctga
     1861 acattcggcg tcagcagcac cgcggccagc gcagccgcgc agtgaaatat ccgttttagg
     1921 ctcattggta atccacctgt aatgttgccc aggcagaaaa ggttccttcg ctgagtgaag
     1981 catcactggc tttcaccggt acagcttgca gcgttggcgg cgttgacagg ttgattttca
     2041 gcggcgtacc caacgtgaac ggctggccat tttgttgcag ctcaatcccc agcccggtga
     2101 cgtttgtctc aatagccgcg ttatcgtagc tggtctggct gcccgtccag ctcagcgtca
     2161 tctcccaggc atcgtcgcgc acgtcgggat cgcatgtaat ggtatagggc acattctggc
     2221 ggaagttatt gccgttgatg ttgtcgataa tgacgtcacg aaactcaact tcaacggttt
     2281 tcccgtcact gatggcacag gcgggcggcg aaaccaatgt gccatgaaat gtgatatcgc
     2341 tatcggccgc ctgtgcaata ccgcaataac aaccgaataa gcagagcgca aatcgtttca
     2401 ttgatactcc accaccattg tcatggttgc gctaaactct gatgccgtaa gctgtgtgcc
     2461 gctttgtttc accggcacgg cttcgaaacc cgggcgcgaa ttaacgttaa acggcagcca
     2521 gttattaccc gccaacgcaa ccggcgcatg gctggacgca tcctgtatgc gaatgccaaa
     2581 accattgatg cccgtactga ggaccgtttc accgttaatg gtggtggtgg tcccctgtaa
     2641 ctgcatctgt aggtcatcgc tgacgctgtt cgaacagttc agggtgtaac caacaggctg
     2701 ccgatagttg ctgccgtcga tgtttttaat aatgacattg ccaaactcca ccgcgctgcc
     2761 ttttaccgta cagggcggcg gtgcatccac cagtatacga agcgttaagt tgaccgtttt
     2821 actatcggcc caggcggagc cgcaggccag taacaccatg ccagcgacgg gtaataaaac
     2881 tcctttcatg ctggatctcc tgtcattagc cttttaggct ttgagcttca tccaccttgc
     2941 aggtgtctcc ttcacaacgg aagaccagcg gtaaacgtgc gccgtagtcg ttgatgtagg
     3001 tcagcaccgg agcgctgttc atttttgcct ttaacggttg tgacgttttc ggcggcatca
     3061 ccagtggttt aaagcccgca gcagttagcc cctttttctg cgtactggca tcgctgatga
     3121 tgacgtggta cggcgtcggg ttattgacga tatagtcctg gccgctgcgc gtgagtgtga
     3181 ccttaaactg ccacggtttt ctcatgtcaa tcttctccag cgcctttggt cgccagaaca
     3241 gcttgatgcg ggtttgcagc gcaatttgca gggtgttggc tttcccggac tttggcggga
     3301 tctcacgcac attgtagtag aacaggcttt cacggtcaga cggcagcgtg tgaatgtccg
     3361 gcagcgcctg aatcttcacc tgaccgttca tcatggcgtc aatacgctgc accggcggca
     3421 gtaccgccag cggcgaagtg attttgttgc ccttctcgtc ttccagccag ctttgcgcca
     3481 gataaggcag cttttcgtta ttgttgcgca gggttacgct tatcgattta tcactttcgt
     3541 taaacaccag acgggtgcga tccggagtga ctgatgcgtg cgccgccagg ttggtggtgg
     3601 tcaacagtac cgccagcgtc gtgctgacca ccattgaaca ggataattta taaaacattg
     3661 attgttcctt aatcatatta ttgagtggct gaaattgacg tcggcgcttc agatggcgtg
     3721 acctgccgag tctggtcctg aataacgggc ttaatttccg gcgctaaaga atcgggggct
     3781 gtcgttccct tttgttcaca cggcaacaac aggccgcgga acagatcggc aggtagcggc
     3841 ttcggcaata cgatctcgca ttgtgcgctg ccttcccaga acaccatcat atgctcaccc
     3901 gcattgatcc ccgccagata gacattgcct tcatcatcca caatgccaac ctgctgctgc
     3961 tcgtcgtttt ttacttccgc gccaaacggc gggtagctac cgtcacgcaa ccgcagtacc
     4021 gccatcgctt tttgcccgct gatcactttg aatttacgat agccgattgc gccttccgtc
     4081 agagtcgcct gcaccaccga ctgggtggct tccgcatctt ccggcaagtt gtttaaatct
     4141 atatacgcct gattgcgata gtagttgtta atatcggcga ccaccgcctt gccaaacata
     4201 ttggtgtaca ccggcgcgcc gttgctttcg accggcacgt tggcaatacc gtcggcgtca
     4261 atcagcaggc gtgtgccgcc catgttttga gtgcgatgca gcgccccacc gtgcgcggtc
     4321 agcgttgccc cgccctgtaa ggcgatcccc gccgaacggt attctccttc atggtagttg
     4381 gcgctgagat ctaccttcgc caacgtgccg tcgtggctca gataaccatc cacgctaccg
     4441 tgctgttcgc tggtgccgac gttaatctgg tagtgcgttg cgtcatcgat gcgattaaag
     4501 tagccgacct gactgctgtc cgacccgcta ccgtaagagc cgttgtaggt cacggtgctg
     4561 ctgtcgctcc acggaatgct catgctgagg tacattccct tatccgtatt atcgtcatat
     4621 tcgtagcgat agccggtcac cgagatgctc atattgcgaa tgctccccat attaaaatag
     4681 tgggaaaaca tcaggttata gtttgtctgt tccgggcgat cccagtaagt acgatgggag
     4741 tagttgagat agaccgagac acccgctgcg gcaaagttct ggttatacgt gatcgtatac
     4801 atctctttgt cgtttccggt ccgcgccatg tccgactggc tcgcgtccag atactcgctc
     4861 atagtcataa agttcttttc ggagaaacgg tagcctgcaa aggtgacgcg gctgttaagt
     4921 tcgtcaaagt ctttggcata gctcacgcga aaggagttgc cgtccagttt tcctttgccg
     4981 tatgcgctgt catgatccag gttgacgtgc gagtgagtca catcgaacgc cagcgcgcca
     5041 aactgtgcga ggtcgcgccc cacccccatc gccgccgact ggtagtgctc atctgccagc
     5101 gcgccaccgt aaagcgacca gccatctgcc accccccatg atgcttctcc gccggaaaaa
     5161 aatccgcctt cggtcttgtg gttccagtct tccggacgcc ccatcatcac tttgtaacgc
     5221 acctgcccct gacgcgtgag gaatggcatg gatgcggtgg tgacgtcata ttcctgcacc
     5281 tgaccattct gttcttcaac acggacgtgt agtgtgccag agacagaatc gccgatatcc
     5341 tgaatgcgga atggcccggc gggaacctgg gtttcgtaaa gtacccggcc catctggctg
     5401 atggtcactt ttgcgctgct gtgcgccacg ccagaaacat ctggcgcata gccgcgcaga
     5461 ttgggcggca gcatctggtc atcggtgctg acactgctgc cgatatagtt aaagccgtcg
     5521 aaaatatcgg aattgagata atcttctccc agcgacagct tcgcttttaa agagggtaaa
     5581 gctcgccagg cgtaataacg gctccagtcc cagtttttac tcgttgtact gttactgctg
     5641 tcatcttcat cgtcgttgct gcgggtgtgc tgataatcac tttgccagtc tgcacggaaa
     5701 cgccatgccc caaggtttgc cccaactgta ccgttaccgc tgatatcatg tgagtcctcg
     5761 cccccttgct cctgatggcg agtttgcgca ttgaggctgt agtcggcaat cagcccagaa
     5821 ataccatcgt cccagcgtga gggagggtcc cagtcgctgc tggtatattc gaggtaagcc
     5881 tgtggcactg tcagcagcaa agccgactgg cttaaatcat tctcgacttc catcccgtct
     5941 aattgccccg gtttaaggca ttcatcgttg tgcgtccact gcaggctttt tgctatccct
     6001 tctttcagcc ccagcgccgc cactaactca ggtgtcaggc aggcataagt ttttgttgga
     6061 tcgttttcag agacatacca attaatgtcg tattcatcag aaagggattg tttatttata
     6121 aatacacgta aattatattt acccgcgtcg acataccctt tttttgaaaa cttaccgaga
     6181 tcaattttcg tgtcgccctt taactcaagg aaacgggggt caaattgaat ggaatcatca
     6241 gcccatgcat tgactgatcc accagagatt gccagcgcaa tatagcaggc gattccccgc
     6301 agccgaaaat ttgagtgatt aggcatacac atatccgttt gtgaaacagt cgtaaatccg
     6361 ttgtgcgttg aataacccca cagcagaacg ctgtggggtt gtcacatcag agataggtaa
     6421 tctggaatgt ggtcagggtt tcgaactggc cgagatccgg cgcatcggtt tcacccacca
     6481 gccaggcttt aaagttcagc gtttgcttgt ctttaccctt gctagttgaa gcatccatca
     6541 cgattggctg aacaatcttt tcgcccactt tataaccttt gccatgggta tcgccgatta
     6601 ccaggctcac gtggttcatg gcaacaccgc tgtcaacatt gaataacgca tagttatctg
     6661 cgttggctgg ccccgcgtag ttagtgccgg tgaacgtggt ctccatgttt gattcagtgg
     6721 taaacacgca atcctgtaaa tggatctgga aatctttcgg tgtggtagtg ccgccgccag
     6781 tcagtttagc cgcaccgata tcgcccatat cgacttccag tttatgatcg tctggaacga
     6841 tggagcacgg ggagtcttca atagtgccgt aaaaacgtgc ggtaccgttg ttacctgcgg
     6901 caaaagcaga actggagacg actgcgccca tgaccattgc ggcagcaatg gctgttttgg
     6961 caaacttact cattattt
//
LOCUS       DAPQZD010000218         6975 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPQZD010000218 DAPQZD010000000
VERSION     DAPQZD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.220"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1049
                     /locus_tag="RO454_004525"
     CDS             <1..1049
                     /locus_tag="RO454_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDY0936248.1"
                     /translation="IYNKNGNKVDFYGKMVGERIWSNTDDNNSENEDTSYARFGVKGE
                     TQITSELTGFGQFEYNLDASKPEGENQEKTRLTFAGLKYNELGSFDYGRNYGVAYDAA
                     AYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSDFFGLVDGLDFAIQYQGKNSNRST
                     KKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQVADGNGSNAELWSLAAKYDANNVY
                     AAVMYGETRNMTPGSIDTGVADQEGNTIMRDQLINETQNFEAVVQYQFDFGLRPSLGY
                     VYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNKNMNVYTAYKFNMLDKDDAAITGA
                     AADDQFAVGIVYQF"
     gene            1642..2493
                     /gene="hchA"
                     /locus_tag="RO454_004526"
     CDS             1642..2493
                     /gene="hchA"
                     /locus_tag="RO454_004526"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDY0936249.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2601..3959)
                     /gene="hprS"
                     /locus_tag="RO454_004527"
     CDS             complement(2601..3959)
                     /gene="hprS"
                     /locus_tag="RO454_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDY0936250.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(3959..4630)
                     /gene="hprR"
                     /locus_tag="RO454_004528"
     CDS             complement(3959..4630)
                     /gene="hprR"
                     /locus_tag="RO454_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDY0936251.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4763..5176
                     /gene="hiuH"
                     /locus_tag="RO454_004529"
     CDS             4763..5176
                     /gene="hiuH"
                     /locus_tag="RO454_004529"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDY0936252.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5285..6289
                     /gene="msrP"
                     /locus_tag="RO454_004530"
     CDS             5285..6289
                     /gene="msrP"
                     /locus_tag="RO454_004530"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDY0936253.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6290..6925
                     /gene="msrQ"
                     /locus_tag="RO454_004531"
     CDS             6290..6925
                     /gene="msrQ"
                     /locus_tag="RO454_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDY0936254.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
BASE COUNT         1941 a         1642 c         1479 g         1913 t
ORIGIN      
        1 aaatttataa taaaaatggc aataaagttg atttttacgg caagatggtt ggcgaacgca
       61 tctggtcaaa tactgatgat aataacagcg aaaacgaaga tacctcctat gctcgtttcg
      121 gtgttaaagg tgaaacccaa atcaccagcg aactgactgg tttcggtcag tttgaataca
      181 acctcgacgc cagcaagcct gaaggtgaaa accaagaaaa aacccgttta accttcgccg
      241 gtttgaaata taacgagtta ggatcatttg actatggccg taactacggt gttgcttatg
      301 atgccgcagc ttataccgat atgttagttg aatggggtgg cgattcctgg gcttccgctg
      361 acaacttcat gaacggtcgt accaacggtg ttgcaaccta ccgtaactct gatttctttg
      421 gcctggttga tggtcttgat tttgcgattc agtaccaagg caaaaacagc aaccgtagca
      481 ctaagaaaca aaatggtgac ggctatgcgt tgtctgtaga ctataacatc aatggttttg
      541 gtatcgttgg tgcatatagc aaatctgatc gcaccaacga tcaagttgcc gatggaaacg
      601 gttcaaatgc tgaactgtgg tcattggctg caaagtatga cgcgaacaat gtctacgcag
      661 ctgtaatgta cggtgaaact cgtaatatga ctccgggttc tattgataca ggtgttgccg
      721 atcaagaagg caatacaata atgcgtgatc aacttatcaa tgaaacccag aactttgaag
      781 cggttgttca gtaccaattc gactttggtc tgcgtccgtc cttaggttat gtgtactcta
      841 aaggtaaaga tatcaaaggt gtacctggac atagatatgt tgacgctgac cgtgtgaact
      901 acattgaagt tggtacctgg tactacttca ataagaacat gaacgtttat actgcataca
      961 aattcaacat gctggataaa gatgacgccg ccattactgg cgcagcagct gatgaccagt
     1021 ttgcagttgg tatcgtctac cagttctaat cagccaacgc gccgaggcgt gttactgtaa
     1081 aacataacac cagcctgagt tcgtccctca tcgaactcag gcttttttat tggcatttca
     1141 tctccatctg cggcacacgc aattgccaaa gcatatcgat acagcacatt accccgcccc
     1201 tcctggctca ataaaatcta ccagcgtctc ttccagccaa cacccaaatg catttcttta
     1261 tgaattaatc cgcaacaaaa ccgcttccca tatagaaata tttgcaaaaa tagtgcatga
     1321 aaaaaacttt ttccctacca atccgggata taaatattca tcagtaccac aacattccac
     1381 agcactaaat ctctccccat cgcccccaaa cctctgataa tggtctaaaa tcattgaggc
     1441 cacttgcgac gacgttactc aataaggaaa gtttggcgtg aataatattc aacaccagtg
     1501 cagcatcatc attacatact tatgcccgcc aactaccgct ttcgatatat tgactcaatt
     1561 tttcagcctg gttaaaaata gcgaccagct cagtcgcaaa tatagtgact accctaacta
     1621 agcaacaata aggaatacac tatgactgtt caaacaagta aaaacccgca ggtcgatatt
     1681 gctgaagata atgcgttctt cccttcagaa tattcgctta gccaatatac gagtcccgtc
     1741 tctgatcttg atggcgtgga ctatccaaaa ccgtatcgcg gtaaacataa aattctggtg
     1801 atcgccgcgg acgaacgtta tttgccgacc gataacggaa aactgttctc gaccggtaac
     1861 catccgattg aaacgttgct gccgttgtat catctccatg ctgcaggttt cgaattcgaa
     1921 gtggcgacca tttccggtct gatgaccaag tttgaatact gggctatgcc gcacaaagat
     1981 gaaaaagtaa tgccattctt tgaacagcat aaatcgttat tccgtaatcc gaagaaactc
     2041 gcggatgttg tcgccagcct caacgctgat agcgaatatg cagcaatctt tgttcctggt
     2101 gggcatggcg cacttattgg tttaccagaa agccaggacg tggctgccgc tttacagtgg
     2161 gcaatcaaaa atgaccgttt tgttatctcc ctttgccacg gtccggcagc ttttctggca
     2221 cttcgccaca gcgataaccc actgaatggt tattctattt gcgcattccc agacgccgca
     2281 gacaaacaaa cgccagatat tggctatatg ccgggtcatc tcacctggta cttcggcgaa
     2341 gagttgaaga aaatgggcat gaacatcatt aatgacgaca tcaccgggcg agtacataag
     2401 gaccgtaaac ttctcaccgg cgacagtcct tttgcagcga atgcgttggg taaactggcg
     2461 gcgcaggaaa tgctggcagc ttacgcgagt taatccctgt tacattgtaa tgtaagcgca
     2521 taatcaatcg cagccacgtt aatactcacg acgtggctgc gttttttcac ggttaactta
     2581 tggcgtactg aagcccgatg ttaatttctt tgcggtaacg taattcggaa cacgttatgc
     2641 ttactgagat agtgatacgt agcacttcct ccatgtaatt cggcaatcgc tttgactaaa
     2701 gaaaggccaa gtccctgacc tacggaatga cgcgaattat ctccccgcca aaatctacgg
     2761 aagagttttt caggctcatt aattttcgtt ccaggactgg cgatatcaat attaagagaa
     2821 ccgttggcat caagaaaact ggttatatga atacgcgatt tttctggtga atatcgaatg
     2881 gcgttaacaa taagattcga taacattcgt tgcagtaaaa ttttatccgc aaagatttgt
     2941 tgattgcact tgaccttaaa gcaaatctct ttctcgtctg aaagatattc aagatagtcc
     3001 aaaagatttt caacttcctt attgagagaa agcgaatcca gtttcaccaa aacattgttt
     3061 ttgtctgccc tggcaagaaa cagtatattc tctgttaacc gcgaaatatt ttccagctct
     3121 tcgatgtttc cggcaattgt tttttgatat tcagcgatac ttctggtttg actgagcgta
     3181 acctgattct gacccagtaa tgcattaatt ggcgttctaa gttcatgagc gagatcgtca
     3241 gcaaattgac taaggcgctc aaaatctttg actaaagcat gatgcatttt attcaacgcc
     3301 tgcccaagag gttttagttc tctcggtaat gcgttaacct caaccggcac tcggctatcg
     3361 ttataattca gcgcttccgt tacgccactc aactttttga tctctcgcaa ccccgttctg
     3421 attaacaacg gacttaataa tgagcacaat ataatgacga caatgcaaat tataatgcta
     3481 ttaattttat actgttcaag catattatgt ctggctgaag ccaatttagc cacagtaacc
     3541 gttaatgatg gcgaaacttc atcaatgtta attcgtaaag catctatctc tgtatcatta
     3601 ataatgcttc tgtaaatgcc tgctgcgctg attgtctcac tagcaggtat gttatttaac
     3661 atgtcatcac tgacatttgt ccggttaaca attttattga tgccatcacc atgaatgatc
     3721 aagatatcct gactaacatc catcatccgg ttaaaataga caggtaatgt atctggattt
     3781 accccatcaa ttaacaactg cttgatctgc gctgtccggt taatgagtgt tgtatcatcg
     3841 cgccatttca actcacttgc caggccatta tagagagtcc atacaattcc agcgccagca
     3901 acagacagca gaaatataaa aagcaaggtt aaacggacgg ttatagataa tcttttcatt
     3961 atttttttac cgctacgaat gaatagccca tcccccggat tgtggcgatt agcttttcag
     4021 gaaaaggatc atcaactttt gcgcggagcc tgcgaatggc gacatccacc gtattggtat
     4081 cactatcaaa gttgattccc caaatttcac tcgcaataac cgttctgggt ataatctcgc
     4141 cagctctgga ggccagtagc caaagtaact gaaactcctt gcgcgtcagt gtaatactga
     4201 tattgtccct gctcacactt tgactgacag agtccatttt taagccactg atttctaatg
     4261 ttgaattcaa agcgtgatgt tgccttaatt gtgcccgaac ccttgccagc aactcagaaa
     4321 atgaaaaagg ttttaccaga taatcatttg ctccactgtc cagccctctg actctgtcat
     4381 cgacagaatc ccttgctgta aggcaaataa caggagtttg ctttgccgtt cttaacgttt
     4441 gtaagatctg ccagccatcc atacccggaa gcataatatc cagaatgatc aatgcataat
     4501 catccttcag cgcaagataa agcccatctc tgccatcaga aacggcatca atgacataac
     4561 ccgcttcgga aagcccctgc gttacccatt cctgggtcct ttgattatct tcaataagta
     4621 gaatcttcat gaaaataata tgccataaat catatgttat agaaacaacc tggttcatta
     4681 caaaattgta atgctgctgt aaggttaccc tggacgtttt ttcgctatcc tcaaaactca
     4741 ttcacatgac aaggatataa acatgttaaa gcgttattta gtactctcgg tagcaacggc
     4801 agcattttca ttaccttctt tggtttatgc cgcacaacaa aacattctta gcgtgcacat
     4861 tttgaaccag caaacaggaa aacccgctgc cgacgtgaca gtcactcttg aaaagaaagc
     4921 ggataacggc tggttacaac ttaataccgc caaaacagat aaggatggac gaattaaggc
     4981 actgtggccc gagcaaactg cgactactgg cgattaccgt gtcgtattta aaaccgggga
     5041 ctatttcaag aaacaaaatc ttgaaagttt cttccctgag atccccgttg aatttcatat
     5101 taataaagtg aacgagcatt atcatgtgcc tttgttactc agccaatatg ggtattcaac
     5161 ctatcgtggc agttaatgcg taaatatgta aaagctgatc tctgctactg ttagggaggt
     5221 gactccctga cagattaaca gtaaacggct cttgttggct aacgacaaaa aagtgtgatg
     5281 gcttatgaaa aagaatcaat ttttaaaaga atcagatgtt acggccgagt cggtattctt
     5341 tatgaagcgt cggcaggtgt taaaagcact gggcatcagc gcagctgcat tttctttgcc
     5401 tcacgctgcg catgccgatc tgcttagctg gtttaaaggg aacgatcgtc cgcccgcccc
     5461 cgccggaaaa ccgctggagt tcagcaagcc tgccgcctgg caaaataacc tgccactgac
     5521 gccagcagat aaagtctccg gttataacaa cttctatgaa ttcgggctgg ataaagccga
     5581 tcccgctgct aatgctggta gcctgaaaac cgatccatgg acactgaaaa tcagcggcga
     5641 agtggcaaaa ccattgaccc tcgatcatga tgatttaacc cgtcgcttcc cgctggaaga
     5701 gcgtatttat cgtatgcgct gcgtggaagc gtggtcgatg gtggtgccgt ggattggttt
     5761 tccgctacac aaattgctgg cgcttgccga acccaccagc aatgcgaaat atgtcgcttt
     5821 cgaaacgatt tatgcgccgg aacagatgcc aggccagcag gaccgcttta tcggcggcgg
     5881 gctgaaatat ccttatgtcg aaggattgcg tctcgacgaa gcaatgcatc cgctcacact
     5941 gatgaccgta ggtgtttatg gcaaggcgtt accaccgcaa aatggcgcgc cggtgcgact
     6001 gattgtgccg tggaaatatg gctttaaagg gattaaatcg atcgtcagta ttaagctgac
     6061 ccgcgagcgt ccgccaacca cctggaatct ggcagcgcct gacgaatacg gtttttacgc
     6121 caacgttaat ccgcatgttg atcacccgcg ctggtcacag gctaccgaac gatttattgg
     6181 ttcaggcggc attctcgatg ttcagcgcca gccaacacta ctgtttaatg gttacgccga
     6241 ccaggtggca tcgctgtatc gtggcctgga tttgcgggag aatttctaaa tgcgcctgac
     6301 agcaaaacag gtgatatggc tgaaagtttg cctgcatctt gccggattgt tgccgtttct
     6361 ctggctggtc tgggcgatca atcacggtgg actaggtgcc gatccggtga aagatattca
     6421 gcattttacc ggtcgcactg cactgaaatt tttgcttgcg gccttgttaa tcacacctct
     6481 ggcacgctac gcaaaacagc cgttattgat acgcactcgc cgcctgttag gcttatggtg
     6541 ctttgcctgg gcgacactgc atttaaccag ttacgcattg ctggagttgg gcgtgaataa
     6601 tctggcgtta ttaggtaagg agttaattac ccggccttat ttaacgttag gaattatcag
     6661 ctgggttatt ttgcttgctt tagcgttcac ttcaacccag tcgatgcagc gaaaactcgg
     6721 caaacactgg caacagttgc ataacttcgt ctatcttgtc gcgatcctga ccccaataca
     6781 ttatctgtgg tcagtgaaga ttatttcacc gcagccactc atctacgctg ggctggctgt
     6841 actgctttta gccttacggt ataagaagtt gctttccctt ttcaaccggt tacgcaagca
     6901 ggcccacaac aaattatctc tataacttca tattgccgac aaacttcgcg ttatcccggc
     6961 cggatgcggc gcgag
//
LOCUS       DAPQZD010000219         6950 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPQZD010000219 DAPQZD010000000
VERSION     DAPQZD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.221"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            68..1465
                     /gene="ulaA"
                     /locus_tag="RO454_004532"
     CDS             68..1465
                     /gene="ulaA"
                     /locus_tag="RO454_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDY0936255.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFASRAL
                     RAEEDAEKQLAEQSA"
     gene            1481..1786
                     /gene="ulaB"
                     /locus_tag="RO454_004533"
     CDS             1481..1786
                     /gene="ulaB"
                     /locus_tag="RO454_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDY0936256.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1796..2260
                     /gene="ulaC"
                     /locus_tag="RO454_004534"
     CDS             1796..2260
                     /gene="ulaC"
                     /locus_tag="RO454_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDY0936257.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2274..2924
                     /gene="ulaD"
                     /locus_tag="RO454_004535"
     CDS             2274..2924
                     /gene="ulaD"
                     /locus_tag="RO454_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDY0936258.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2934..3788
                     /gene="ulaE"
                     /locus_tag="RO454_004536"
     CDS             2934..3788
                     /gene="ulaE"
                     /locus_tag="RO454_004536"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDY0936259.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            3788..4474
                     /gene="ulaF"
                     /locus_tag="RO454_004537"
     CDS             3788..4474
                     /gene="ulaF"
                     /locus_tag="RO454_004537"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDY0936260.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFVWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4603..4878)
                     /locus_tag="RO454_004538"
     CDS             complement(4603..4878)
                     /locus_tag="RO454_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0936261.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5206..5601
                     /gene="rpsF"
                     /locus_tag="RO454_004539"
     CDS             5206..5601
                     /gene="rpsF"
                     /locus_tag="RO454_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDY0936262.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5608..5922
                     /gene="priB"
                     /locus_tag="RO454_004540"
     CDS             5608..5922
                     /gene="priB"
                     /locus_tag="RO454_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDY0936263.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5927..6154
                     /gene="rpsR"
                     /locus_tag="RO454_004541"
     CDS             5927..6154
                     /gene="rpsR"
                     /locus_tag="RO454_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDY0936264.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6196..6645
                     /gene="rplI"
                     /locus_tag="RO454_004542"
     CDS             6196..6645
                     /gene="rplI"
                     /locus_tag="RO454_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDY0936265.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1674 a         1715 c         1894 g         1667 t
ORIGIN      
        1 attagagtga gagcacaaca ttccgggtgt gcgaaatacc cggttacctc ttcttcagga
       61 gatcgttatg gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa
      121 tgccccgttg ttgctgggta ttgtgacctg tctgggttac atcctactgc gcaaaagtgt
      181 cagcgttatt attaaaggta cgattaaaac cataattggt ttcatgttgt tgcaggcagg
      241 gtccggcatc ctgaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg
      301 cattaacggc gcaatttccg atacttacgc gtcgatgatg gcaaccattg aacgcatggg
      361 cgatgcctat agctgggtgg gttacgcggt gttgttagcg ctggcgctga acatctgtta
      421 cgtgctgctg cgtcgcatta ccggtattcg aaccatcatg ctgactggtc acatcatgtt
      481 ccagcaggcg gggctgattg ccgttacgct gtttatcttc ggctactcca tgtggacgac
      541 cattatctgc accgcgattc tggtttcgct ctactggggc attacctcca acatgatgta
      601 caagccgaca caggaagtca ccgacggctg tggtttctcc attggtcacc agcagcagtt
      661 cgcatcatgg attgcttata aagtcgcacc gttccttggc aaaaaagagg aaagcgttga
      721 agacctcaaa ctgcctggct ggctgaatat tttccacgac aacatcgtct ccacggcgat
      781 tgtcatgact atcttctttg gtgccattct gctctccttt ggtatcgaca ccgtgcaggc
      841 gatggcaggc aaagtgaact ggacggtgta catcctgcaa accggtttct cctttgcggt
      901 ggcgatcttc atcatcactc agggcgtgcg catgtttgtg gcggaactct ctgaagcatt
      961 taacggtatc tcccagcgtc tgatcccagg tgcggttctg gcgattgact gtgcggctat
     1021 ttatagcttc gcgccgaacg ccgtggtctg gggttttatg tggggcacca tcgggcaact
     1081 gattgcggtt ggtatcctgg tcgcctgtgg ctcttcaatc ctgattattc ccggctttat
     1141 cccgatgttc ttctccaacg ccactatcgg cgtgttcgct aaccacttcg gcggctggcg
     1201 tgcggcgctg aaaatttgtc tggtgatggg gatgatcgaa atctttggtt gcgtctgggc
     1261 agtgaaactc accggtatga gtgcctggat gggtatggcg gactggtcga ttctggcacc
     1321 gccgatgatg cagggcttct tctccatcgg tatcgccttt atggccgtca tcattgtaat
     1381 tgcactggct tatatgttct tcgctagccg cgcgctgcgc gcagaagaag atgcagaaaa
     1441 acaactggca gaacaatctg cttaataagg agttttgatt atgaccgttc gtattctggc
     1501 tgtgtgtggt aacggacaag gcagttccat gatcatgaaa atgaaagtgg accagttttt
     1561 aacccagtcg aacattgacc acacggtaaa cagctgcgcg gttggcgaat acaaaagcga
     1621 gttgagcggc gcggatatca tcatcgcttc tacccatatt gcgggcgaaa tcaccgtgac
     1681 cggcaacaaa tatgtggttg gcgtgcgcaa catgctctct cctgctgatt ttggcccgaa
     1741 actgctggaa gtgatcaaag agcatttccc gcaggacgtg aagtaaggac gcgccatgaa
     1801 attacgtgat tcgctggctg aaaataaatc tatccgcctg caagcggaag cagagacatg
     1861 gcaggacgca gtgaaaatcg gcgttgatct gctggtagcg gcagatgtgg tagagccgcg
     1921 ttactaccag gcgattctgg atgcggttga acagcacggc ccgtactttg tgctcgcccc
     1981 cggcctggca atgccgcacg gacgcccgga agagggcgtt aagaaaaccg gttttgcgct
     2041 ggtgacgctg aaaaagccgc tggagttcaa ccacgaagat aacgatccgg tggatatcct
     2101 catcaccatg gcggcggttg atgccaacac tcaccaggaa gtgggcatca tgcagatcgt
     2161 caacctgttt gaagatgaag agaatttcga ccgtttacgt gcctgccgta ccgagcagga
     2221 agtactggat ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aacatgtcat
     2281 taccgatgtt gcaagtcgcg ctggacaacc agactatgga tagcgcctac gaaaccactc
     2341 gcctgattgc cgaagaagtc gacattatcg aagtgggcac cattctttgc gtaggcgaag
     2401 gcgtgcgtgc ggttcgtgac ctgaaagcgc tgtacccgca caaaatcgta ctggctgacg
     2461 ccaaaattgc cgatgcaggc aaaatccttt ctcgcatgtg cttcgaagcc aacgctgact
     2521 gggtgaccgt aatttgttgt gcggatatca acaccgccaa aggcgcgctg gacgtggcaa
     2581 aagagtttaa tggcgacgtg cagatcgaac tgaccggcta ctggacctgg gaacaggcgc
     2641 agcagtggcg cgacgcaggc attgggcagg tggtttatca ccgcagccgt gacgcgcagg
     2701 ccgcaggcgt ggcgtggggc gaagcggaca ttaccgcgat caaacgtctt tccgatatgg
     2761 gcttcaaagt caccgtcacc ggtggtctgg cgctggaaga tctgccgttg tttaaaggca
     2821 ttccgattca tgtgtttatc gctggtcgca gcatccgtga tgcagcctct ccggttgaag
     2881 ccgcgcgtca gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg gatatgttgt
     2941 ccaaacaaat cccgcttggc atctacgaaa aagcgctccc cgctggggag tgctggctgg
     3001 aacgactgca actggcaaaa acgttaggct tcgattttgt cgagatgtca gtagatgaaa
     3061 cggacgatcg cctttcgcgc ctcgactgga gccgcgagca gcgtctggca ctggtgaacg
     3121 cgattgttga aaccggcgtg cgcgtaccgt ccatgtgcct ttctgctcat cgtcgtttcc
     3181 cgctgggaag cgaagatgac gcggtgcggg cgcaggggct ggagattatg cgtaaagcta
     3241 tccagttcgc ccaggacgtc ggcattcgcg tgatccaact ggcgggctat gacgtttact
     3301 atcaggaagc caataacgaa acgcgtcgtc gttttcgtga cggcctgaaa gagagcgttg
     3361 agatggcaag ccgcgcgcag gtcacgctgg cgatggagat catggattac ccattgatga
     3421 actccatcag caaggcgctg ggctacgcac actatctcaa caatccgtgg ttccagctct
     3481 acccggatat cggcaacctg tcggcgtggg acaacgacgt gcagatggag ttgcaggccg
     3541 gaatcgggca tatcgtcgcg gtacatgtga aagacaccaa accaggcgtc ttcaaaaacg
     3601 tgccttttgg cgaaggtgtt gtggattttg aacgttgctt cgaaacgctc aaacagagtg
     3661 gctattgcgg gccgtacctg attgagatgt ggagcgaaac ggcggaagat ccggcggcag
     3721 aagtggcgaa agcgcgcgat tgggtgaaag cgcgcatgac caaagcaggc atggtggagg
     3781 cggtataatg caaaagctga aacagcaggt gtttgaggcc aacatggatc tgccgcgtta
     3841 cgggctggtg acctttacct ggggcaacgt cagcgccatt gaccgcgaac gcgggctggt
     3901 ggtgatcaag cccagcggcg tcgcctacga aaccatgaaa gcagacgata tggtggtggt
     3961 cgatatgagc ggtaaggtgg tggaagggga gtatcgtccg tcttccgaca ctgcgacgca
     4021 tctcgaactc taccgtcgtt atccgtcgct tggtggtatt gtccataccc actccactca
     4081 tgccaccgca tgggcgcagg cggggctggc gatcccggcg ttaggcacca cgcacgccga
     4141 ttacttcttt ggcgacatcc cgtgcacgcg cggattaagc gaagaagagg tgcagggtga
     4201 gtacgaactg aataccggca aagtgattat cgaaacgctg ggtgacgccg agccgctgca
     4261 tacgccggga attgtggtgt atcagcacgg accgttcgtc tgggggaaag atgcccacga
     4321 tgcggtgcat aacgcggtgg tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag
     4381 tattaaccca caactgaatc acatcgacag ctttctgatg aataaacact tcatgcgtaa
     4441 gcacggtccc aacgcctatt acgggcagaa gtaaaacacg cgctgcggaa atttccttct
     4501 tcgggagata actggtctaa ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg
     4561 gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc
     4621 gctggcgtgc atattgccgt tactccccgg ttcccgcatc agaatcacat ggtagtacgt
     4681 tgcgccttgc gcatctgtct cttcatttaa tgcctgacgt gcttcacttt cagtggcgaa
     4741 gttatggttg atataaatca cacctaagct ttgcacatca tccatatttc tggcctggtg
     4801 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
     4861 taaaacacga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctcttgt
     4921 ggtgatcttc tgatttaatt ttaatcaatg ataaagaagt tgatggtgac catttctgat
     4981 gcagttgttc aaaaaaacac catgacgaag tgtgatgaac tccaaatcag cgatttagag
     5041 gttatttgcg aaaggggcga tttatttctg ctcagccctt gagtttagcg gggtatgtca
     5101 gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc cccgggattc
     5161 ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg tcattacgaa
     5221 atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat cgagcgctac
     5281 actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg gggccgccgt
     5341 cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat gaacgttgaa
     5401 gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga tgccgttatc
     5461 cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat ggttaaagcg
     5521 aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa
     5581 gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc cggcaccgtg
     5641 tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt
     5701 gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcgtggtg ccaaatgccc
     5761 gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt cggcagtcgc
     5821 ataaccgttc aggggttcat ttcatgccat aaggcaaaga acggactgag caaaatggtt
     5881 ttgcatgccg agcagattga attgatagat tctggagact agccatatgg cacgttattt
     5941 ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg actataaaga
     6001 tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa gccgtatcac
     6061 cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct
     6121 gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt aatacgactt
     6181 tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct gggtagcctg
     6241 ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc gcagggtaaa
     6301 gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa
     6361 gctaaactgg ctgaagttct ggcagcagct aatgctcgcg ctgagaaaat caatgcactg
     6421 gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg ttccatcggt
     6481 actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa gagcgaagtt
     6541 cgtctgccga acggcgttct gcgtaccact ggtgaacacg aagtgagctt ccaggttcac
     6601 agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt tattcaacga
     6661 gacgtaaaaa cgccgaccag tggtcggcgt tttgctttta agtgataaaa accgcagaaa
     6721 cagacgttcc ctccataaat ctcttgctat tccttttata aaaagttcgc agtacccata
     6781 cctggccccc agcctcgccg ctggtagttc gtgtcatgtt ttttacgcgt aaaacacgac
     6841 aaaagcatca atacagcgat cgtctcactt gtatgataat aaaaacaata tgttatatat
     6901 gaatgtgctg ttattttatt gtttttatat ataaaaagat aaaatatcat
//
LOCUS       DAPQZD010000220         6947 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPQZD010000220 DAPQZD010000000
VERSION     DAPQZD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.222"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            486..1475
                     /gene="araF"
                     /locus_tag="RO454_004543"
     CDS             486..1475
                     /gene="araF"
                     /locus_tag="RO454_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDY0936266.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1545..3059
                     /gene="araG"
                     /locus_tag="RO454_004544"
     CDS             1545..3059
                     /gene="araG"
                     /locus_tag="RO454_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDY0936267.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTNMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3074..4060
                     /gene="araH"
                     /locus_tag="RO454_004545"
     CDS             3074..4060
                     /gene="araH"
                     /locus_tag="RO454_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDY0936268.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4227..5027
                     /gene="otsB"
                     /locus_tag="RO454_004546"
     CDS             4227..5027
                     /gene="otsB"
                     /locus_tag="RO454_004546"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDY0936269.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5002..6426
                     /gene="otsA"
                     /locus_tag="RO454_004547"
     CDS             5002..6426
                     /gene="otsA"
                     /locus_tag="RO454_004547"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDY0936270.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6433..6861)
                     /gene="uspC"
                     /locus_tag="RO454_004548"
     CDS             complement(6433..6861)
                     /gene="uspC"
                     /locus_tag="RO454_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDY0936271.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
BASE COUNT         1731 a         1631 c         1819 g         1766 t
ORIGIN      
        1 tttttcattt ttgcgatatg gctctcatta tacgtgttct gttaattaat gagcacagtg
       61 ataattaatg gcgaaatgaa tgacgcgcat tttccacatc tttaagttgc ggttattaca
      121 ccattgcaaa aactcaacgc caggtaatga ggcctattga ctggttaaaa agaagacatc
      181 ccgcatgggt accaaagaca acaaggactt ccaggctaat cttatggatt attctgctgt
      241 gcactcgata atttgtctga caaattcgct tttccttatg tcttttcccg ctaaatttat
      301 gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa ttaatttctc
      361 gctaaaacta tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc tttgttttcc
      421 gattaattta acgaatgtca ttcgtttttg ccctacacaa aacgacacta aagctggaga
      481 gaaccatgca caaatttact aaagccctgg cagccattgg tctggcagcc gttatgtcac
      541 aatccgctat ggcggagaac ctgaagctcg gtttcctggt gaagcaaccg gaagagccgt
      601 ggttccagac cgaatggaag tttgccgata aagccgggaa ggatttaggg tttgaagtta
      661 ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc tatcgacagc ctggccgcca
      721 gtggcgcgaa aggtttcgtt atttgtactc cggacccgaa actcggctct gccatcgtgg
      781 cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt gttaacgcca
      841 aaggtaagcc aatggatacc gttccgttgg tgatgatggc tgcgaccaaa attggcgaac
      901 gtcagggcca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc aaagaaagcg
      961 cggtgatggc gattacctcc aacgaactgg atacggctcg tcgccgtact acggggtcta
     1021 tggatgcgct gaaagctgca ggattcccgg aaaaacaaat ttatcaggta ccgaccaaat
     1081 ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcaa catccggaag
     1141 ttaaacattg gctgattgtc ggtatgaacg acagcaccgt gctgggcggc gtacgcgcga
     1201 cggaaggtca gggctttaaa gcggccgata tcatcggtat cggtattaac ggtgtggatg
     1261 cggtgagtga actgtctaag gcgcaggcaa ccggcttcta cggttccctg ctgccaagcc
     1321 cggacgtaca tggttataag tccagcgaaa tgctttacaa ctgggttgca aaaggtgttg
     1381 aaccgaccaa attcaccgaa gttaccgacg tggtgctgat cacgcgtgac aactttaaag
     1441 aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt cccctctgca
     1501 tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa cagtctaccc
     1561 cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg ctgacggata
     1621 ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat ggcgcaggaa
     1681 aatcaactct cttaaaaatc ctcagcggaa actatgcgcc aaccacgggt tctgtagtga
     1741 ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg ggtgtggcga
     1801 ttatttatca ggaactgcat ctcgtgccgg aaatgaccgt cgcggaaaat atctatctcg
     1861 gccagctgcc gcataaaggc ggcattgtga atcgttcatt gctgaattat gaggcaggtt
     1921 tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa tatctctcca
     1981 ttggtcagtg gcagatggtt gaaatcgcca aagcgctggc gcgtaacgcc aaaattatcg
     2041 cctttgatga gccaaccagc tccctctctg cccgcgaaat cgacaatctt ttccgcgtta
     2101 ttcgtgaatt gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt atggaagaaa
     2161 tatttgccct cagcgatgcc atcaccgtct ttaaagatgg gcgttatgtc aaaactttta
     2221 ccaatatgca gcaggttgac cacgacgcac tggtgcaggc gatggtcggg cgagacattg
     2281 gcgatatcta cggctggcaa ccgcgtagtt atggcgagga gcggctgcgt cttgatgctg
     2341 tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtgga gaaattgtcg
     2401 gtctgtttgg tctggtcggg gcagggcgta gcgaattaat gaaaggcatg tttggcggga
     2461 cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt cgtaaaccga
     2521 gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg gaaggcatta
     2581 ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaaa catgtgcttg
     2641 gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac attcgttcgc
     2701 tcaacatcaa aacgccgggc gctgagcaac tgatcatgaa tctctcaggc ggaaatcagc
     2761 aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg ctggatgaac
     2821 ctacgcgtgg cattgatgtt ggtgctaagc atgaaattta caacgtgatt tatgcgctgg
     2881 cggcgcaggg tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc ctcggcgttg
     2941 ccgaccggat tgtggtgatg cgggaaggcg aaatcgccgg tgaattgtta cacgagcagg
     3001 cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagccaggca gttgcctgag
     3061 taaggagagt atgatgtctt ctgtttctac atcgggttct ggcgcaccta agtcgtcatt
     3121 cagcttcggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg tgctttttat
     3181 cgcctgtgcc atttttgtcc caaattttgc caccttcatt aatatgaaag ggttgggcct
     3241 ggcaatttcc atgtcgggga tggtggcttg tggcatgttg ttctgccttg cttccggtga
     3301 ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg cggtggttat
     3361 taatctgact gaaagcctgt ggattggcgt ggcagcgggg ttgctgctgg gcattctctg
     3421 tggtctggtc aatggctttg ttatcgccaa actcaaaata aatgccctga tcacgacact
     3481 ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta aagcggtcgg
     3541 tatcgaagat gaaagcttct ttgcccttgg ttacgccaac tggttcggtc tgcctgcgcc
     3601 aatctggctc accgtcgcgt gtttgatcat ctttggcttg ctgctgaata aaaccacctt
     3661 tggtcgtaat accctggcga ttggcgggaa cgaagaggct gcgcgtctgg cgggtgtacc
     3721 ggttgttcgc accaaaatta ttatctttgt tctctctggc ctggtatcag cgatagccgg
     3781 aattattctg gcttcacgta tgaccagtgg gcagccaatg acgtcgattg gttatgagct
     3841 gattgtgatc tccgcctgcg ttttaggtgg cgtttctctg aaaggcggca tcggaaaaat
     3901 ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gagaacgcca tgaacctgct
     3961 taatatttct cctttcgcgc agtacgtggt tcgcggctta atcctgctgg cagcggtgat
     4021 cttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc tgcaacaatt
     4081 tagcgctttt ttcctccaca gccagccgcc acaacggttg gctgttcttc gttacaaatg
     4141 gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg cgcaacacaa
     4201 taagaaaaga gaaggaggag aaccgggtga cagaaccgtt aaccgaaacc cctgaactat
     4261 ccgcgaaata tgcctggttt tttgatctcg atggaacgct ggcggaaatc aaaccgcatc
     4321 ccgatcaggt cgtcgtgcct gacactattc tgcaaggact tcagctactg gcaaccgcaa
     4381 gtgatggtgc attggcattg atttcagggc gctcaatggt ggagcttgac gcactggcaa
     4441 aaccttatcg cttcccatta gcgggcgtgc atggggcgga gcgccgtgac atcaatggta
     4501 aaacgcatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg caactgcata
     4561 cggtcatcgc ccagtatccc ggcgcggagc tggaggcgaa agggatggct tttgcgctgc
     4621 attatcgcca ggctccgcag catgaagacg cattaatgac attagcgcaa cgtattactc
     4681 agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc aaaccgaaag
     4741 gtaccagtaa aggtgaggcg attactgcct ttatgcagga agctcccttt atcgggcgaa
     4801 cgcccgtatt tctgggcgat gatctaaccg atgaatctgg cttcgcagtc gttaaccggc
     4861 tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg cgactggcgg
     4921 gcgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa caaaaaagag
     4981 aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg gattgcacca
     5041 ccagacgagc acgccgccag tgccggtggc cttgccgttg gcatactggg ggcactgaaa
     5101 gccgcaggtg gactgtggtt tggctggagc ggtgaaacag ggaatgagga tcagccgcta
     5161 aaaaaggtga aaaagggtaa cattacgtgg gcctctttta acctcagcga acaggacctt
     5221 gacgaatact acaacaaatt ctccaatgcc gttctctggc ccgcttttca ttatcggctc
     5281 gatctggtgc aatttcagcg tcctgcctgg gatggctatc tacgcgtaaa tgcgttgctg
     5341 gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca cgattatcac
     5401 ctgttgccct ttgcgcatga attacgcaaa cggggagtga ataatcgcat tggtttcttt
     5461 ctgcatattc ctttcccgac gccggaaatc ttcaacgcgc tgccgacata tgacaccttg
     5521 cttgaacagc tttgtgatta tgatttgctg ggtttccaga cggagaacga tcgtctggcg
     5581 ttcctggatt gcctttctaa cctgacccgc gtcacgacac gtagcgcaaa aagccatacg
     5641 gcctgcggca aagcgtttcg aacggaagtt tacccgatcg gcattgagcc gaaagaaata
     5701 gcgaaacagg ctgccggtcc actgccgcca aaactggcgc aactcaaagc agaactgaaa
     5761 aacgtacaaa atatcttttc tgtcgaacga ctggattatt ccaaaggttt gccggagcgt
     5821 tttctcgcct atgaagcgtt actggaaaaa tatccgcaac atcacggtaa aattcgttat
     5881 acccagatcg cgccaacgtc tcgtggtgat gtgcaagcct atcaggatat tcgtcatcag
     5941 ctcgaaaatg aagccggacg aattaatggt aaatatgggc aattaggctg gacgccgctt
     6001 tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac
     6061 gtgggcttag tgacgccact gcgtgacggg atgaacctgg tggcaaaaga gtatgttgcc
     6121 gctcaggacc cagccaaccc gggcgttctt gttctttcgc aatttgcggg agcggcaaac
     6181 gagttaacgt cggcgttaat tgttaatccc tacgatcgtg acgaagttgc agctgcgctg
     6241 gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac
     6301 gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag
     6361 atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt tcctaagctt
     6421 gcgtaggtgc ggttaacctc ccgtaagtgg aactaacagc acatcgacct cacttgaggt
     6481 aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca
     6541 aatcaccaaa tcgaaatgat gtttgcgaca aacttccaga atatgttcgc ttaactcacc
     6601 gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa
     6661 gctttgcgtt tcttcctgca tcacgttgcg taaatcttca agcatcggcg cagctaattg
     6721 attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct
     6781 ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacggcgac
     6841 aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa
     6901 gctttactcc taatggagaa acgacgcaat cccaactcag ttaaaca
//
LOCUS       DAPQZD010000221         6842 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPQZD010000221 DAPQZD010000000
VERSION     DAPQZD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.223"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(339..2009)
                     /gene="betA"
                     /locus_tag="RO454_004549"
     CDS             complement(339..2009)
                     /gene="betA"
                     /locus_tag="RO454_004549"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDY0936272.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFNIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2023..3495)
                     /gene="betB"
                     /locus_tag="RO454_004550"
     CDS             complement(2023..3495)
                     /gene="betB"
                     /locus_tag="RO454_004550"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDY0936273.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3509..4096)
                     /gene="betI"
                     /locus_tag="RO454_004551"
     CDS             complement(3509..4096)
                     /gene="betI"
                     /locus_tag="RO454_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDY0936274.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4225..6258
                     /gene="betT"
                     /locus_tag="RO454_004552"
     CDS             4225..6258
                     /gene="betT"
                     /locus_tag="RO454_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDY0936275.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1448 a         1801 c         1851 g         1742 t
ORIGIN      
        1 cgtgtttaaa gctccatgtg gttttggtct gacttatttt tgtactcgtt acaagaaccc
       61 ttttcgtata tgctaaccgt gagcgggaat gaacaggtaa ccatagccag gaacattagc
      121 ggcgtaattc ttaagctgag tggtatttca tctgtagttt atctgtgtat gcatagcaca
      181 ttcatcagcc agattgagca agtcagctta gaatttgctg gcgagattgt cacgatgaag
      241 catgagagtt acctcagtgt tttatataag gattcggtcc tcatagtaga acggtaactc
      301 tcaatctgat aggttcctga gtcagttcac atcacgactc attttttcgc tctcaccggc
      361 atcccatttg ccacaaaata tcccgccgtg ctcctcggca acgcttcctt cccacgaatc
      421 atatccgcta ttttctcgcc aatcataatc gtcgtggcgt tcaaattgcc ggtgataatt
      481 tgcggcataa ttgacgcatc caccacgcgc aggccttcca gcccgtgtac gcggccttcg
      541 ccgtcaacca cagccatctc gtcataaccc attttgcagg taccgcacgg atgaaacgcg
      601 gtttcggcgt ggttacgcac gaactcatcg agctgctcat ccgtctggca ttcgacacca
      661 gggctgattt cgcggccacg gtattgatcc agcgcgggtt gatgcatgat ctcgcgtgtg
      721 atgcgaattg cgtcgcggaa ctcctgccag tcctgctcgt gcgacatgta gttaaacaga
      781 attcccggat gctggtgcgg gtcgcgggat ttaatccgca catgcccacg gcttggcgag
      841 cgcatcgatc cgacatggca ttggaagccg tgctctttca ctgcgttcga gccgttatag
      901 ttaatcgcca ctggcaggaa atggtactga atattcggcc acgcaaattc ctcacggctg
      961 cgaataaatc cgcctgcttc aaagtggttg ctggcaccaa cgccggtgcc gccaaacagc
     1021 cactccgcac cgattctcgg ctgattccac cactgcaggg cagggtagag ggaaaccggt
     1081 tctttgcact cgtattgcag atacatctcc aggtgatcct gaagattttc gccgacgccg
     1141 ggtaattcat gcaccagcgg aatattaaac tccgccagca gttcagcgtt gccgacgccg
     1201 gagcgttgca ggatctgcgg tgaagcaatc gcgcctgcac ataacagcac ttctttattc
     1261 gccgccgcgc gggtcggaat agtgctgtcg ccttccagcc actcgacgcc caccgcgcgt
     1321 ttgccgtcaa aaatgatgtg atcggtcatg gcgtgagtac gaatggtcag gttagggcgc
     1381 gatttggcct gatcgagata gccacgcgcg gtgctggcgc gacggccatg cggcgtgacg
     1441 gtgcgatcca tcgggccaaa accttcttgc tgatagccgt tgagatcgtc cgtgcgtggg
     1501 tagcctgcct gcatgcccgc ttcaatcatc gcttcaaaca gcggattgac gccgggtttg
     1561 gaggtgatga cgctcaccgg gccatcgcca ccgtggtaat cgttctcgcc cacatcgcga
     1621 gtctcggcct tgcggtagta gggcaggcag tcgagataac tccagttctc cagaccaggt
     1681 tcttgtgccc agttatcgag atccagcgca ttgccacgga tgtagcacat accgttgatc
     1741 agcgatgaac cgcccagacc tttgccgcgt ccgcactcca tgcggcggtt gttcataaac
     1801 ggttcgggtt ccgtctcata ggcccagttg tagcgtttgc cctgtagcgg gaacgccagc
     1861 gcggcgggca tctgggtgcg gaagtcaaag cgatagtccg ggccgcctgc ttcaagcagc
     1921 agcacggttg tgttcggatc ttcagtcaga cgggtagcga gaacgttgcc ggctgagccg
     1981 gcaccaataa tgatgtagtc aaattgcaaa taaacctcct ggttagaata tggactggaa
     2041 tttagccatc tcaacctgga tggacttcac ctgggtgtaa ctctggagcg tcatcacgcc
     2101 gttctcgcga ccaatgccgg agtgtttgta gccgccaacg ggcatctctg ccggggattc
     2161 gccccaggtg ttgatccagc aaatacccgc ttccagctga tgaatgacgc gatgcgcgag
     2221 gttcaggtcc gccgtcacga tacccgccgc cagaccgtag tcggtatcgt tagcgcggcg
     2281 aatgacttcg tcttccgact cgtagatcag aatggacatc actggcccga aaatctcttc
     2341 tcgcacaatg gtcatatcgt cgctgcaatc ggtgaacacg gtcggtgcaa cccatgcgcc
     2401 gttatcgaag ctatccccct tcagtacatt gccgccgcac agtacgcgcg cgccttcctc
     2461 tttgcctttg gcgatatagc gcagcacgtt atcgcgatgc gggaagctga ccagcgggcc
     2521 gaagttagtt tgcggatcga aaacgtcgcc cgcgcgaatg cgctcaacgc gcgccagaac
     2581 cttttgttca aatgcggctt tgcatttcgt cggaacgaag acgcgagtgc cgttggtaca
     2641 cacctgaccg gagctgaaga aattcgccat catggcgata tcggcggcga gatcgagatc
     2701 cgcatcgtcg aaaacgatca gcggtgattt accgcccagc tccatggtca cttctttcag
     2761 ggaggaagct gccgagttag ccatcacttt tttgccgctg gcgacaccgc cggtaaatga
     2821 cactttggca atgcccggat gatcggtcag atattgtccg gtctccgcgc ccacgcccgg
     2881 caacacgtta aatacgccgt ccggcaggcc cgcttcgctg taaatttcgg ccagctttaa
     2941 cgcggtaagc ggggtgactt cgctcggttt gaaaatcatt gcattgcctg ccgccagcgc
     3001 tggggcggat ttccacaaag caatctggat cgggtagttc catgcgccaa tccccgccac
     3061 cacacccagc ggttcgcggc gggtataaac aaatgacgtc tcacgcaacg ggatctggct
     3121 gccttccagc gccgggatca gcccggcata gtactccagc acgtccgcac cggtaacgat
     3181 atcgacggtt gaggtttccg aatatgcttt tccggtgtca agggtttcca gttttgcgag
     3241 ttcgtcattg cgttcacgca gaatatcaac ggcccgacgc agaatacgcg agcgctccat
     3301 agcggtcatc gccgcccaga ttttttgccc ttgttgggca ctcttcacgg cgcgatcgac
     3361 atcctcacgc ccggcggcct gcacggtcgc cagcacgtta ccgttggccg ggttaatggt
     3421 ctcgaaggtg cgaccgctgg tggcggaggt ataaccacca tgtatataaa gctgctgttc
     3481 tgccattcgg gacatcacgt ctcctcggtt aatcggtggg taaatgctga gtaataaagt
     3541 gacgggtcag cgagtgtgcg agaggtttat ccagcggttt gccgctcagt gccgcgcgca
     3601 gccataatcc gtcaattagc gcggcaaggc cgtagcccgc ttcctgtgcc tgttggcgcg
     3661 gtaactcgcg gcgaaattcg ctcaccagat tcgacaataa gcggcggcta ctgacctgct
     3721 gcaaacggta aagcatcggc tgatgcatac tgctggccca gaaggccagc caggctttca
     3781 tcgccgcact gctcacctgg gtttcatcga agtttccgcc aacaatcgcc tgtaatcgca
     3841 gctctgcact gccctgcgga agtgcatgta atcgattcaa aaccgcgtca cgcagctgac
     3901 tggtgatatc gcgcatggtt gcttccagca gaccattttt gtccctgaaa tagtggctga
     3961 tgatccccgt agaaacgcct gcacggcggg cgatctgcgc gatcgttgca tcgtgcatgc
     4021 ccacttcatt tattgcttcc agcgtggcgt cgatcagttg tctgcgccgg atcgactgca
     4081 ttcccaattt gggcattttc gccactccat tcatcagcgt tgtttatcta ttaaagcggt
     4141 tattgattgg acgttcaata taaaatgtgt cttaattgtt actaatttga ttttaaatgg
     4201 taacaataac agtggggata ctggatgaca gacctttcac acagcaggga aaaggacaaa
     4261 atcaatccgg tggtgtttta cacttccgcc ggactgattt tgttgttttc cctgacaacg
     4321 atcctgtttc gcgacttctc ggccctgtgg attggccgca cgctggactg ggtttctaaa
     4381 accttcggtt ggtactatct gctggcggca acgctctata ttgtctttgt ggtctgtatc
     4441 gcttgttcgc gttttggttc ggtgaagctc gggccagaac aatcaaaacc ggaattcagt
     4501 ttgctgagtt gggcggcgat gctgtttgct gccgggatcg gtatcgacct gatgttcttc
     4561 tccgtagccg aaccggtaac gcagtatatg cagccgccgg aaggcgcggg acagacgatt
     4621 gaggccgcgc gtcaggcgat ggtctggacg ctgtttcact acggcttaac cggctggtcg
     4681 atgtatgcgc tgatgggcat ggcgctcgga tactttagct atcgttataa tttgccgctc
     4741 accatccgct cggcgctgta cccgatcttc ggtaaacgga ttaacgggcc gataggtcac
     4801 tcagtggata ttgctgcggt gatcggcacc atcttcggta ttgccactac gctcggtatc
     4861 ggtgtggtgc agcttaacta tggcttgagc gtgctgtttg atattcccga ttcgatggcg
     4921 gcgaaagcgg cactgatcgc cttgtcggtg atcatcgcca ctatctcggt gacatccggt
     4981 gtcgataagg gcattcgcgt gttatcggaa ctcaacgttg cgctggcgct gggattgatt
     5041 ctgttcgtgt tgtttatggg cgacacctcg ttcctgctta atgcactggt gctgaacgtt
     5101 ggcgactatg tgaatcgctt tatgggcatg acgctcaata gttttgcctt cgaccgtccg
     5161 gttgagtgga tgaataactg gacgctcttc ttctgggcat ggtgggtggc atggtcgccg
     5221 tttgtcggct tgttcctggc gcgtatctcg cgtgggcgta ccattcgcca gttcgtgctg
     5281 ggcacgttga ttattccgtt taccttcacg ctgttatggc tctcggtatt cggcaatagc
     5341 gcgctgtatg aaatcatcca cggcggcgcg gcatttgccg aggaagcgat ggtccatccg
     5401 gagcgcggct tctacagcct gctggcgcag tatccggcgt ttacctttag cgcctccgtc
     5461 gccaccatta ctggcctgct attttatgtg acctcggcgg actccggtgc gctggtgctg
     5521 gggaatttca cctcgcagct taaagatatc aacagcgacg cacccggctg gctgcgcgtc
     5581 ttctggtcgg tggcgattgg cctgctgacg ctaggcatgc tgatgaccaa cgggatatcc
     5641 gcgctgcaaa acaccacggt gattatgggc ctgccgttca gctttgtgat cttcttcgtg
     5701 atggcggggt tgtataaatc tctgaaggta gaagattacc gccgtgaaag tgccaaccgc
     5761 gataccgcac cgcgaccgct ggggcttcag gatcgcctga gctggaaaaa acgtctctcg
     5821 cgcctgatga attatccggg cacgcgttac actaaacaga tgatggagac ggtctgttat
     5881 ccggcaatgg aagaagtggc gcaggaactg cggttgcgcg gcgcgtacgt ggagctaaaa
     5941 aacctgccgc cggaagaggg acaacagttg gggcatctgg atttgttggt gcatatgggc
     6001 gaagagcaaa actttgtcta tcagatttgg ccacagcaat attcggtgcc gggctttacc
     6061 taccgcgcac gtagcgggaa atcgacctac taccggctgg aaaccttcct gttagaaggc
     6121 agccagggca acgacctgat ggactacagc aaagagcagg tgatcaccga tattctcgac
     6181 cagtacgagc ggcaccttaa ctttatccat ctccatcgtg aagcgccggg ccatagcgtg
     6241 atgttcccgg acgcgtgatt gttattgcat aaaaccgcgc catgtctgca tatggcgcgg
     6301 tttctcttac ctcttgatac gttatatctg tgtacagtta agcccttagt atcgattgat
     6361 gattgccagg caacagataa taaaaaagaa aagaactatt gaagcccaaa acctacgctt
     6421 ggactgttgt gaatgttcaa taagcttagt cttatttaat gtaaatattg gtgctaattt
     6481 gaaatgacgc tttattcatg ggaaacgcgt agcgcaaatc aaccaaaatc aatgtaaccg
     6541 tttgttgttt aaggtttaat tgtctgtgtc tgatatttta ttgattgttt taaatattgt
     6601 ttttattgtc attgttataa tgttggctat ctattgctga atggattcag tcttaaaaag
     6661 caggttgtta agggattagc atagagtaat gacgtgtatg cataaccaac atctttactc
     6721 attatgtcat tgaatgttga cgctatgtgt ttatgaggtt gaggtatttt cagttgatct
     6781 ggattgttaa attcatataa tgcgccttta ctcatgaatg gacgtcaata cgtagtggga
     6841 aa
//
LOCUS       DAPQZD010000222         6793 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPQZD010000222 DAPQZD010000000
VERSION     DAPQZD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.224"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            21..584
                     /locus_tag="RO454_004553"
     CDS             21..584
                     /locus_tag="RO454_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDY0936276.1"
                     /translation="MLKKTLLSMFATALLSGVAFNALADDPNQGSGKITFKGEVIDAP
                     CSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSS
                     ASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNF
                     KARMESLGRTATPGNVQAQANYVLDYK"
     gene            669..3191
                     /locus_tag="RO454_004554"
     CDS             669..3191
                     /locus_tag="RO454_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0936277.1"
                     /translation="MNNKNTFTGDTLSHAIKNALSGVVCSLLFVLPAHAVEFNVDMID
                     VEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQALILEWVKADNESGSLLCLTKE
                     DLTSFGLNTEFIESLQTIAGRECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQKQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYADNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVVPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3207..3947
                     /locus_tag="RO454_004555"
     CDS             3207..3947
                     /locus_tag="RO454_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDY0936278.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRGYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVTPFASATQYLNNLPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            3944..4993
                     /locus_tag="RO454_004556"
     CDS             3944..4993
                     /locus_tag="RO454_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDY0936279.1"
                     /translation="MITLFRLLAILCLFYNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSDVGIDTFACLDKYEQYYGGIYHDPVCNGSTLQKNVTFNARFRAYV
                     KFKARPAVDQTWDFGVVNVLQFDGEGGANLAADNKNLRYIIDGLNNILFLDCSVDVRI
                     FPESQIVNFGQISANSIATYRPKAAFSVSTIKDVAADCTEQFDVVTSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5047..5700)
                     /gene="ribB"
                     /locus_tag="RO454_004557"
     CDS             complement(5047..5700)
                     /gene="ribB"
                     /locus_tag="RO454_004557"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDY0936280.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5804..5952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6074..6373
                     /gene="ubiK"
                     /locus_tag="RO454_004558"
     CDS             6074..6373
                     /gene="ubiK"
                     /locus_tag="RO454_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDY0936281.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPAAELQSPPAIPPVDKAE"
     gene            complement(6408..6608)
                     /gene="glgS"
                     /locus_tag="RO454_004559"
     CDS             complement(6408..6608)
                     /gene="glgS"
                     /locus_tag="RO454_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDY0936282.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1939 a         1604 c         1492 g         1758 t
ORIGIN      
        1 atctataaag gattatagtc atgttaaaaa aaacattgtt atctatgttc gcaaccgcat
       61 tgttatctgg cgttgctttt aacgcgcttg ctgatgaccc aaatcagggt tctggtaaaa
      121 taacatttaa aggtgaagtt atcgatgcgc cttgttctat tgctcctggt gatgaagatc
      181 agacaataaa cctcggtgaa gttgctgata ctgtattaaa aagcggacag aaatctcttc
      241 ctgtagatgt caccattcac ttgcaggatt gtattttatc tgacggcact aacactgttg
      301 ataaagtcaa aatcaccttt agttctgcca gtgttgacgc taccgactcc aacctgctta
      361 aaaacactct ggaaggtaac atcggcggcg caactgatgt aggcgtacgt ctggtgaaat
      421 cagacaacac caacgtgact cttggcactc caatcactat caacttcccg acgactaact
      481 cctaccagga gttgaacttt aaagcccgta tggaatctct gggacgcacc gcgaccccgg
      541 gtaacgtgca ggcacaggcc aattacgtac tcgactacaa gtaagcggca attaatacgc
      601 acaacgtcag acggtaaagc cgatgatatg tcatcggcaa ctttgggtct tttttgaaat
      661 ggatttttat gaacaataaa aacacgttta ccggggatac attatcccac gcaattaaaa
      721 atgccctgtc tggcgtagtg tgttcgctac tcttcgtttt acctgcccat gcagtggaat
      781 tcaacgttga tatgattgat gttgaagacc gtgagaatat cgacatctct cgttttgaga
      841 aaaaaggcta tatcccccca ggcagatacc tcgctcgtgt gcaaataaat aaaaatatgt
      901 tgccacaagc gttaatactg gaatgggtaa aagccgataa tgaaagtggt tcgttactct
      961 gcttaaccaa agaagatctg actagtttcg gtcttaatac ggaatttatt gaatcattgc
     1021 aaaccatagc tggcagagaa tgtctcgatt taagccaacg tcaggagtta acgacacggc
     1081 ttgataaagc tacgatgata ttatcgctaa gtgttcccca ggcgtggtta aaataccagg
     1141 caacaaactg gacgccacca gagttttggg ataccggtat cgccgggttt atccttgatt
     1201 acaatgtgta cgccagccaa tatgccccac atcatggaga cagcacccaa aacgtcagct
     1261 cctatggtac gttaggcttt aacctcggcg catggcgctt acgtagcgat taccaatata
     1321 atcagaattt tgctgatgga cgctcggtaa accgcgacag cgaatttgcg cgaacttatc
     1381 tgtttcgccc tatcccctcc tggtcgtcaa aattcactat gggccagtac gacctgagct
     1441 ccaatcttta cgataccttc cactttactg gcgcatcgct ggaaagtgat gaaagcatgc
     1501 tgccgccaga tttacagggt tatgcgccac aaattaccgg catcgcgcag accaacgcga
     1561 aagtaactgt ggcacaaaat ggtcgtgtac tttatcaaac cactgtcgcg ccaggccctt
     1621 ttactatttc tgatttgggg caatcgtttc aggggcagct ggatgtcaca gtggaagaag
     1681 aagatggccg caccagcacc ttccaggttg gatccgcatc cattccctat ttaacccgta
     1741 aagggcaagt gcgctataaa acgtcgctgg gaaaaccgac atccgtcggg cataacgata
     1801 tcaataatcc ctttttctgg acggcggaag cctcctgggg ctggttgaac aatgtgtcgc
     1861 tgtatggtgg cggcatgttc accgctgatg attatcaggc tatcactacc ggtattggct
     1921 ttaatcttaa ccaattcggt tcgctttctt ttgatgtcac cggagcagac gcgtctttgc
     1981 agaaacaaaa tagcgacaat ctgcgtggtt acagctatcg cttcaactat gcaaagcatt
     2041 tcgaatcgac aggtagtcag attaccttcg cgggttatcg cttctcagat aaagattacg
     2101 tgtcgatgag tgagtaccta agctcgcgta atggcgatga gtcaattgat aatgaaaaag
     2161 agagttatgt catttccttg aaccagtact ttgaaacact ggaattaaac tcttatctca
     2221 acgttacacg caatacttat tgggacagcg ccagcaatac caactactcc gtatctgtaa
     2281 gcaaaaactt tgatattggc gatttcaaag gtatctctgc atcgctggca gtaagtcgaa
     2341 tccgctggga tgacgacgaa gagaatcaat attacttctc tttctctcta cctttacaac
     2401 aaaaccgcaa catctcctac agtatgcagc gtacgggaag cagtaatact tcgcagatga
     2461 tttcctggta cgattcatca gatcgcaaca atatctggaa tatttcagcg tcggcaacgg
     2521 acgacaatat acgtgatggc gaaccaacac tgcgcggcag ctaccagcac tattcaccgt
     2581 ggggacgtct gaacattaac ggcagtgtac agccgaatca gtacaattct gttaccgcag
     2641 gctggtacgg ttcacttacc gctacacgtc atggtgtcgc ccttcacgat tatagctatg
     2701 ctgataacgc ccgcatgatg gtcgataccg acggcatctc cggcgttgaa atcaactcta
     2761 accgaactgt taccaacggg ctgggcattg ccgttgtacc ttcgttatcg aactacacca
     2821 cctccatgtt gcgggtgaac aataacgatc tgccagaagg tgtcgatgtc gaaaactcgg
     2881 ttattcgtac tacgctcacc cagggtgcca tcggctacgc aaaactgaat gccaccaccg
     2941 gataccaaat cgtcggcgtt attcgtcagg aaaatggccg cttccctcca ctaggtgtga
     3001 atgtcacgga taaagcgaca ggtaaagatg tgggcctggt cgcggaagac ggcttcgttt
     3061 atctcagcgg tattcaggaa aacagtattc tgcatttaac ctggggtgat aatacctgtg
     3121 aagtcacgcc gccaaaccaa agtaacatta gcgaaagcgc aataatttta ccttgtaaaa
     3181 cagtcaaata attcaggatg aatataatga aatcaccatt aatcacaaca ggaatgattt
     3241 gtttactggg gatctgcaat tttgcccagg cgactgtctc ccccgacagg acacgtatca
     3301 tttttaatgc ttcgaataaa agcgcaacgg tccgtctgac taaccaaagt aaaatcgatc
     3361 cctatctggc tcagtcgtgg attgaagatg ccagcggtaa aaaaacgcgt ggttacatat
     3421 caacactgcc gccgatggaa cgtattgagc cggatgaaca ggtacaaatt cgcctgatgg
     3481 cgctggcctc attaaacaac ttaccgcagg acagagaaac gctgttctac tataacgtgc
     3541 gggaaattcc gcctcgcact aaagaacaga atgtgatgca aatcgccatg cagagtcgac
     3601 tgaaattatt ctggcgtccg aaagcaatcg aattaaaaga agggcagatg gtgcctttac
     3661 aaaaagtcac aataacacgg accgctgcag ggctgacact caataatcca acgccttatc
     3721 acatcaccgt cgggtatatc ggtactaacg gcaagacact gatgcccggt gcagacagca
     3781 ttatggtgac tccgtttgct tctgcaactc aatacttgaa caacttgccc tcaactttcc
     3841 agttaggctt tgttgctgac tatggtggac tggagatgtt taaagtcgaa tgtaattccg
     3901 tccaatccct ctgccaaagc tctcctgcta agaagggaaa aatatgatta cgcttttccg
     3961 tttactggcg attctttgcc ttttttataa cgtttcagtt tatgctgtaa attgctatca
     4021 ggatcaaaga ggtggtaata cacaaatacg tggtacttta ccttccttca gaattccaga
     4081 aaacgcccag ccgggacaaa aaatttggga aagcgatgac gttaatgtta cggtatattg
     4141 tgataacgcg acaggatgga gagctgatga tcccgcagaa aatatttatg cctggattaa
     4201 aatgccggct ataaatagtg cagacatttt gaataaccca tacctgacat tcggtgtaac
     4261 ctataatggt gtggactatg aagatagcga tgtaggaatt gatactttcg cctgtctgga
     4321 taagtatgaa cagtattatg gtggtattta tcatgatcca gtttgtaatg gcagcaccct
     4381 acaaaaaaat gtcacgttta atgcccgttt tcgcgcatat gtgaaattta aagcacgtcc
     4441 agcagtggat caaacctggg attttggcgt ggtaaatgtt ctgcaatttg atggtgaagg
     4501 tggtgctaac ttagcagcag ataataaaaa tctacgttat attattgatg gattaaataa
     4561 tattttattt cttgattgta gtgtcgatgt ccgtattttt ccagaaagcc agatcgttaa
     4621 tttcgggcaa atctctgcaa actctattgc tacttatcga cccaaagctg catttagcgt
     4681 atctactata aaagatgttg ctgccgactg taccgagcaa tttgatgttg tcaccagttt
     4741 ttatacgaca gatacgttac atgatgatac gcatctggaa atgggtaatg gtttgttaat
     4801 gcgtattact gatcaaaaaa cacaagaaga cattaagttt aataagtaca aacgcttcac
     4861 cacatatatt ccaggtcaga ctggagcgat ggttacccgt gattaccagg ctgaactttc
     4921 acaaaaaccg ggggagactc tcgtttatgg gcctttcaaa aaagatctca tcgtaaaaat
     4981 aaactataac tgaacagcaa aatgccggga acaccccggc attttcatat ttagtatttc
     5041 acgtcattag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
     5101 cacgagcgcc atattgtgtt tattcgcaaa ctcaatgcac tctggtgcac gcgccatcgt
     5161 gccatcgtca ttagtcagct cacacagcac accagctggt ttaaagcctg ccagcgtcat
     5221 cagatcgata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
     5281 aagtgggaaa acgtggcctg ggcgattcag atctgaaggt tttgcgccat cggcaatcgc
     5341 tgcgcgaacg gtcgtaatac ggtcagcggc agaaacaccg gtagtcacac cttcagctgc
     5401 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
     5461 cagatcgagt tgtttacggc gatcgtcagt aatgcacagg caaacaatac cactcccgtg
     5521 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
     5581 ttcgttttca cggtcttcat catcaagcac catcacaccg cgtccttcac gcagcgcagc
     5641 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
     5701 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
     5761 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
     5821 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
     5881 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
     5941 ctgagaataa gcggattcac tataacgcta atgattagcg ccagcaacgc atagcttcac
     6001 ataattctgg tttatgactt acccttatcg cactacaatg gcactcaaca cctatcagta
     6061 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga
     6121 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
     6181 cctacaagcg cagctgacgc gtctcgatct ggtaagccgc gaagaattcg acgttcagac
     6241 gcaagtcctg ttacggactc gtgaaaaact ggcgttactg gagcagcgca tcagcgaact
     6301 ggaagcgcgc aataatccgg cagcagaact tcaatctcct ccggctatcc cgccagttga
     6361 taaagcagaa taaagcacca caaaaacggg ccagacggcc cgtaatatca gtgttctaat
     6421 tcaagttctc tggtttgcat catctgttgg caataccagg cataacgtgc gcaaaaccag
     6481 gttctatgtt cttcatccat gttaccagta acggccagaa tatcgaccgg gcgaccttct
     6541 gcgtgcattc tggcaaaact acgcgcgaga aaatcgaaat tatttaaaga attaagacta
     6601 tgatccataa gcacatcctc cggttattgc atacctttta aggccatatg ctttttctga
     6661 tttcagtatt ccccaattag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc
     6721 ccgatcacac tttttagtga aaaataaaaa aaccatatta aaagactttc tcttccagaa
     6781 cttctcaaac atg
//
LOCUS       DAPQZD010000223         6781 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPQZD010000223 DAPQZD010000000
VERSION     DAPQZD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.225"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            352..1692
                     /gene="dcuB"
                     /locus_tag="RO454_004560"
     CDS             352..1692
                     /gene="dcuB"
                     /locus_tag="RO454_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDY0936283.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1770..3416
                     /gene="fumB"
                     /locus_tag="RO454_004561"
     CDS             1770..3416
                     /gene="fumB"
                     /locus_tag="RO454_004561"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDY0936284.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3539..4189
                     /locus_tag="RO454_004562"
     CDS             3539..4189
                     /locus_tag="RO454_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDY0936285.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWLAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4186..5541)
                     /gene="melA"
                     /locus_tag="RO454_004563"
     CDS             complement(4186..5541)
                     /gene="melA"
                     /locus_tag="RO454_004563"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDY0936286.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEDYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5824..6732
                     /gene="melR"
                     /locus_tag="RO454_004564"
     CDS             5824..6732
                     /gene="melR"
                     /locus_tag="RO454_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDY0936287.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAILNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         1664 a         1818 c         1681 g         1618 t
ORIGIN      
        1 acccacaaat gggtagatca gattaatcta taaacctaat aatacctgcc ctgagaacaa
       61 aaaacagacc gataaatatc aataagataa cagcaaacaa aacattaaca tctgcgctgt
      121 acaaactata aacccatcgc cagagagtct ttctctctga aaaagccgct tatcacagtg
      181 cataaatttg ccgctgcttt aatcagccaa tattcactgt gaggtatttg ctcaagccgg
      241 taacgaccaa acggatattt agtcaggctc tgaaaacagt tcatacaaaa cagaacgtga
      301 ctgtgatcta ttcagcaaaa atttaaatag gattatcgcg agggttcaca catgttattt
      361 actatccaac ttatcataat actgatatgt ctgttttatg gtgccagaaa gggtggtatc
      421 gcgctgggtt tattaggcgg tatcggtctg gtcattctgg tcttcgtctt ccaccttcag
      481 ccaggtaaac caccggttga tgtcatgctg gttatcattg cggtggtggc ggcatcggcg
      541 accttgcaag cttcgggcgg tcttgatgtc atgctgcaaa ttgccgagaa gctgctgcgt
      601 cgcaacccga aatatgtctc aattgtcgcg ccgtttgtga cctgtacgct gaccattctt
      661 tgcggtacgg gtcatgtggt ttacaccatt ctgccgatca tctacgacgt tgctattaag
      721 aacaacatcc gtccggaacg tccgatggcg gcaagttcta tcggtgcaca gatggggatt
      781 atcgccagtc cggtgtcggt tgcggtcgta tctctggttg cgatgctggg taatgtcacc
      841 tttgatggtc gccatcttga gttcctcgac ctgctggcaa tcaccattcc atcgacgtta
      901 atcggtatcc tggcgatcgg tatcttcagc tggttccgcg gtaaagatct ggataaagac
      961 gaagagttcc agaaattcat ctccgtaccg gaaaaccgtg agtatgttta cggtgatacc
     1021 gcgacgctgc tggataaaaa actgccgaaa agcaactggc tggcaatgtg gatcttcctc
     1081 ggggcaatcg ctgtagtcgc ccttcttggt gctgattcgg acctgcgtcc atccttcggc
     1141 ggcaaaccgc tgtcgatggt actggttatt cagatgttta tgctgctgac cggggcgctg
     1201 attattatcc tgaccaaaac caatcccgcg tctatctcaa aaaacgaagt cttccgttcc
     1261 ggtatgatcg ccatcgtggc ggtgtacggt atcgcatgga tggctgaaac catgttcggt
     1321 gcgcatatgt ctgaaattca gggcgtactg ggtgaaatgg tgaaagagta tccgtgggcc
     1381 tatgccattg ttctgctgct ggtttccaag tttgtaaact ctcaggctgc ggcgctggcg
     1441 gcgattgttc cggtcgcgct ggcgatcggc gttgatccgg catacatcgt ggcttcagca
     1501 ccggcttgct acggttatta catcctgccg acttatccga gcgatctggc agcgattcag
     1561 tttgaccgtt ccggcaccac ccacatcggt cgcttcgtca tcaaccacag ctttattctg
     1621 ccggggttga ttggtgtgag cgtatcgtgc gtcttcggct ggatcttcgc cgcgatgtac
     1681 gggttcttat aaatgcactt tgcgtgccgc ccgtaactac gcggcacgcc attttcgaat
     1741 aacaaataca gagttacagg ctggaagcta tgtcaaacaa accctttatc taccaggcac
     1801 ctttcccgat ggggaaagac aataccgaat attatctact cacttccgat tacgttagcg
     1861 ttgccgactt cgacggcgaa accatcctga aagtggaacc agaagccctg accctgctgg
     1921 cgcagcaagc ctttcacgat gcttctttca tgctccgccc ggcacaccag aaacaggttg
     1981 cggctattct tcacgatcca gaagccagcg aaaacgacaa gtacgtggcg ctgcaattct
     2041 taagaaactc cgaaatcgcc gccaaaggcg tgctgccgac ctgccaggat accggcaccg
     2101 cgatcatcgt cggtaaaaaa ggtcagcgcg tgtggaccgg tggcggtgat gaagaagcac
     2161 tgtcgaaagg cgtctataac acctatatcg aagataacct gcgctattca cagaacgcgc
     2221 cgctggacat gtataaagag gtcaacaccg gcacgaacct gcctgcgcaa atcgacctgt
     2281 acgcggtaga tggcgatgag tacaaattcc tctgtgtcgc taaaggcggc ggctctgcca
     2341 acaaaacgta tctctaccag gaaaccaaag ccctgctgac gcccggcaaa ctgaaaaact
     2401 tcctcgtcga gaaaatgcgt accctcggta ctgcagcctg cccgccgtac catatcgcgt
     2461 ttgtgattgg cggtacgtct gcggaaacca acctgaaaac cgtcaagtta gcaagcgctc
     2521 actattacga tgaactgccg acggaaggga acgaacatgg tcaggcgttc cgcgatatcc
     2581 agctggaaca ggaactgctg gaagaggcac agaaactcgg tcttggcgcg cagtttggcg
     2641 gtaaatactt cgcgcacgac atccgcgtta tccgtctgcc acgtcacggc gcatcctgcc
     2701 cggtaggcat gggagtctcc tgctccgctg accgtaacat taaagcgaaa atcaaccgcg
     2761 aaggtatctg gatcgaaaaa ctggaacaca acccaggcca gtacattcca caagaactgc
     2821 gtcaggcagg tgaaggcgaa gcagtgaaag tcgaccttaa ccgcccgatg aaagagatcc
     2881 ttgcccagct ttcgcaatac ccggtatcca cccgcttgtc gctcaccggc accattatcg
     2941 tgggtcgcga tattgcacac gccaagctga aagagctgat tgacgccggt aaagaactgc
     3001 cgcagtacat caaagatcac ccgatctact acgcgggtcc ggcgaaaacg cctgccggtt
     3061 atccatcagg ttcacttggc ccaaccaccg caggtcgtat ggactcctac gtggatctgc
     3121 tgcaatccca cggcggcagc atgatcatgc tggcgaaagg taaccgcagt cagcaggtta
     3181 ccgacgcgtg ccataaacac ggcggcttct acctcggtag tatcggcggt ccggcggcag
     3241 tactggcgca gcagagcatc aagcatctgg aatgcgtcgc ttatccggag ctgggtatgg
     3301 aagctatctg gaaaatcgaa gtagaagatt tcccggcgtt tatcctggtc gatgacaaag
     3361 gtaacgactt cttccagcag atcgtcaaca aacagtgcgc gaactgcact aagtaacctc
     3421 ttcggcccag cgcctggcag catgctgcca ggtgatcccc ctgggccacc tcttttgcga
     3481 ttgtaatttc acgcttgctg gtgaatagtc agtattttcc ctgacttgcg aactcaccat
     3541 gacccgcaca ctcaagccgt taattcttaa cactggcgca ctggcgctaa cgttaatcct
     3601 gatttatacc ggcatttcgg cccatgacaa actcacctgg ctgatggaag tgacaccggt
     3661 gattattgtc gtgccgctac tgcttgccac cgctaaacgt tatccgttaa cgccgctgct
     3721 ttatacgctc attttctttc acgccatcat cctgatggtc ggcgggcaat acacctacgc
     3781 gaaagtcccc gttggttttg aggtgcagga atggctgggg ttgagccgta atccgtatga
     3841 caaactggga cattttttcc aggggctggt gcctgcactg gtggcacgag agatccttgt
     3901 acgcgggatg tacgttcgcg gacgtaagat ggtggcgttt ctggtctgct gcgtggcgct
     3961 ggcgataagc gcgatgtatg aattaatcga gtggttggca gcgctggcaa tgggccaggg
     4021 agctgatgat tttctcggca cccagggcga ccagtgggac acgcaatccg atatgttctg
     4081 cgcgctgctt ggcgcattaa cgacggtgat attcctcgct cgctttcatt gccgccagct
     4141 acggcgcttt ggcttaatca ccagagcgcc ggaagcgatt accccttaac ggtgcaacca
     4201 gcctggcagc cagtcgccgt gggcggcaat caggtcgtca acaagagcat atatttcgtc
     4261 aatgcccagc acggcggcag tatgcgggtc catcatcgcg gcgtggtaaa cacggtcgcg
     4321 attttccgta agaatagctt ctgtcagcag cgtctgtacg ttgatgttgg tttgcatcag
     4381 ggcggccaga tgcgaaggta gcgtaccggc tttggtcggc tgaatgccat tagcatcaac
     4441 cagacaggcc acttccacgc aacatccttg tggcaggtta tcaatcaaac catcgttacg
     4501 gacattgcca taaatcacac tcggctcgcc agtccagata gcgttcatga ttgtgctggc
     4561 atattcccgt gacggtttaa tatcaatccg ggaggcgttt ttataatcct ccagctcttt
     4621 atgccagttc gccagctgct cgacgcagcg tttcgggtac tcatccagcg gtactttata
     4681 acgctcaatc aaatcctcac gacctggctt aataaaccac ggtgtgtact cagcaaaatg
     4741 ttctgacgat tctgtgacga agtagcccag ctttttgaac atttcgtaac gcacaatatt
     4801 ctggcagcga gtattaccat gaatatttgg cttcggtgcc tgccctgctt cataagccgc
     4861 cagcaattca ggatagagat tcacgtagct accgtctgcg gttttacgct ccagttccag
     4921 gtaaaacgcc atatggttga tacctgcgca acggtaacgc agcgtagccg gatcgatatt
     4981 gaggtcacgc gccagctctt ccgccgttcc ctgcaccgaa tggcacagcc ctacctgctt
     5041 gatatgcgga tagcgtgcat acatcgccca ggtattcatc gccattgggt taacgtagtt
     5101 gagcatggtg gcatcggggc agacttccgt catgtcctcg caaatctgcc acaggtgcgg
     5161 aatggtacgt agcgcacgca taatgccgcc cggccccagc gtatcggcaa tggtttgttc
     5221 cagaccatgc cgcttacaga cctcgaaatc agtcaccgtg caaggttcat aaccgccaat
     5281 ctgaaatgcc accaccacaa aatcagcatc ctgtaaggct tctttctgtt gggtgtggca
     5341 ggtgattttg ccgctggccc ctgctgaatc catcaacttg cgcaccacaa tgtgtgactc
     5401 ttccaggcgg gtgggatcga tatccatcag ggcaatatgc gccgttttca gcgcctcacg
     5461 atggaacaca tcaccaagaa tatttttaac gaaaatcgtc gaaccagcgc cgataaatgt
     5521 aattttaggt gcagacatca tgcagatctc ctggcttgct tgaatgactt catcatggca
     5581 ggcgaatctg cagaaaacct ccggcttcca ggttgagcat tcccataaac tcatatccta
     5641 agtggcacat caatagatct gagtttatgg gaataaatta cctgaaaagc tgaataatcg
     5701 accccaatct tctcatccgc tctcctcgca cctccaggga gaggaataaa accgtgcgcc
     5761 tattcgcagt catcctccct cactcctgcc ataattctga tattccagga aagagagtca
     5821 tccatgaata cagatacgtt tatgtgcagc agcgacgaaa agcagacccg tagcccactg
     5881 tcgctgtatt cagaatacca gcggatggaa attgagtttc gcgcaccgca tatcatgccc
     5941 accagccact ggcatggtca ggtcgaagtg aatgtgcctt tcgatggcga tgtggaatac
     6001 ctgatcaaca atgaaaaagt gaatatcaat cagggtcata tcacgctgtt ctgggcctgt
     6061 acaccgcacc aactaacaga taccggaacc tgtcagagca tggcgattct taatctgccg
     6121 atgcatctgt ttctctcctg gccgctggat aaagacctga ttaaccacgt tactcacggc
     6181 atggtgatca aatcactggc gacacagcaa cttagcccgt ttgaagtgcg ccgctggcag
     6241 caggaattaa acagtccgaa cgagcaaatt cgccagctcg ccattgatga aattggcctg
     6301 atgctcaagc gatttagcct ctctggctgg gaaccgattc tggtcaataa aacctcgcgc
     6361 acacacaaaa acagtgtctc gcgccatgcg caattttatg tcagccagat gctgggcttt
     6421 attgccgaaa actatgatca ggcgctgacc atcaacgatg tggctgagca cgtcaaactt
     6481 aacgccaact atgcaatggg gatattccag cgggtcatgc aattgacgat gaaacagtac
     6541 ataaccgcga tgcgcatcaa ccacgttcgc gcattactga gcgataccga taaaagtatt
     6601 ctcgatattg ccctgacggc aggctttcgt tcgagtagcc gtttttacag cacgttcggc
     6661 aaatatgtcg gcatgtcgcc gcaacaatac cgcaaactta gccaacagcg ccgccagacg
     6721 tttcccggct aaagacgata tcagtatcag ccaaaaaaat agtttagccg tcgataataa
     6781 g
//
LOCUS       DAPQZD010000224         6711 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPQZD010000224 DAPQZD010000000
VERSION     DAPQZD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.226"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1135
                     /gene="emrK"
                     /locus_tag="RO454_004565"
     CDS             <1..1135
                     /gene="emrK"
                     /locus_tag="RO454_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDY0936288.1"
                     /translation="SDRRKYFALLAVVLFIALSGAYAYWSMELKDMISTDDAYVTGNA
                     DPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLY
                     LQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAA
                     IQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGE
                     TVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGIN
                     MGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNED
                     IAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1135..2673
                     /gene="emrY"
                     /locus_tag="RO454_004566"
     CDS             1135..2673
                     /gene="emrY"
                     /locus_tag="RO454_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDY0936289.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2781..4109)
                     /gene="dsdA"
                     /locus_tag="RO454_004567"
     CDS             complement(2781..4109)
                     /gene="dsdA"
                     /locus_tag="RO454_004567"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDY0936290.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4127..5464)
                     /gene="dsdX"
                     /locus_tag="RO454_004568"
     CDS             complement(4127..5464)
                     /gene="dsdX"
                     /locus_tag="RO454_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HDY0936291.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            5682..6617
                     /gene="dsdC"
                     /locus_tag="RO454_004569"
     CDS             5682..6617
                     /gene="dsdC"
                     /locus_tag="RO454_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HDY0936292.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1851 a         1536 c         1550 g         1774 t
ORIGIN      
        1 ttctgacaga agaaaatatt ttgctttgtt agcggtagtt ttatttattg cgctttcagg
       61 tgcctatgcc tattggtcaa tggaattaaa agacatgatt agtacagatg acgcctatgt
      121 cacgggaaat gcagatccaa tttctgcaca agtctcaggc agtgtcactg tcgttaatca
      181 taaagatacg aactacgttc gacaaggtga cattttagtt tcactggata aaactgatgc
      241 cactatcgca ctcaataaag ctaaaaataa tctggcaaat attgttcggc aaacgaataa
      301 actatactta caggataaac aatacagtgc cgaagtcgct tcagcacgta ttcagtatca
      361 acaatcttta gaagattata accgtcgtgt gcctttagcg aagcaagggg ttatttcaaa
      421 agaagcgctg gagcatacca aagatacgtt aataagtagc aaagcggcat tgaatgctgc
      481 tatccaggct tataaagcga ataaagcttt agtaatgaac acaccattaa accgtcagcc
      541 acaagtcatt gaagctgcag atgcaaccaa agaagcctgg ttggcgctta aacgtacgga
      601 tattaagagt ccggttaccg gttatattgc ccagagaagt gttcaggtcg gcgaaacagt
      661 gagtcccgga caatcgttaa tggctgtcgt accggcgcgt caaatgtggg ttaatgccaa
      721 ctttaaagaa acacaactca cggatgtacg gattggtcaa tcggtcaata ttatcagcga
      781 tctttatggt gaaaatgttg tgtttcatgg tcgggtgaca gggatcaata tgggaaccgg
      841 caatgcgttc tccttattac ctgcacaaaa tgcgacaggg aactggatca aaatcgttca
      901 gcgtgtaccg gttgaagttt ctctggatcc aaaagaactc atggaacacc ctttgcgcat
      961 tggtttatcg atgactgcaa ctattgatac gaagaatgaa gacattgccg agatgcccga
     1021 tctggcttca accgtgacgt ccatgcctgc ttataccagt aaggctttag ttatcgatac
     1081 cagtccgata gaaaaagaaa ttagcaacat tatttcgcat aatggacaac tttaatggca
     1141 atcactaaat caactcctgc accattaacc ggtgggacgt tatggtgcgt cactattgca
     1201 ttgtcattag cgacgtttat gcaaatgttg gattccacta tttctaacgt cgcaataccg
     1261 acaatatctg gctttctggg agcatcaaca gacgaaggta cctgggttat cacctcgttt
     1321 ggcgtagcaa atgccattgc gatccctgtt accggcaggc tggcacaaag aataggcgaa
     1381 ttaaggttat ttttactttc agtaaccttt ttttcgctgt cttcattaat gtgtagttta
     1441 tcgaccaatc ttgatgtgct gatatttttt agagtcgttc aggggttaat ggcggggccg
     1501 ttaattccac tgtcacagag tttattatta aggaattatc caccagaaaa aagaacattt
     1561 gctctggcat tatggtcaat gaccgtgatt atcgctccga tatgtgggcc gatattgggc
     1621 ggctatattt gcgataactt tagctggggt tggatatttt taatcaatgt ccctatgggg
     1681 attatcgtcc tgacattatg cttaacctta cttaaaggac gagaaactga gacttcaccg
     1741 gtcaaaatga atctaccagg actgaccctg ttagtgctcg gtgttggtgg cttgcaaatt
     1801 atgcttgata aagggcgcga tctggattgg ttcaactcga gtacaataat actactaaca
     1861 gtagtatcag ttatttctct gatctcttta gtcatttggg agtcgacctc agagaatccg
     1921 attcttgacc tcagtttgtt taagtcccgt aatttcacca ttggtattgt gagtatcaca
     1981 tgcgcgtatt tattttactc tggagcgata gttcttatgc cgcagttact ccaggaaacg
     2041 atggggtata atgcgatatg ggccggactt gcctatgcgc ccatcggtat catgccgcta
     2101 ttaatatcac ctttgatagg acgttatggc aacaaaattg atatgcggtt gttggtgaca
     2161 tttagttttt tgatgtatgc ggtttgctat tactggcgtt ctgtgacatt tatgccaacg
     2221 attgatttta caggcatcat tttgccacag ttttttcagg gattcgccgt tgcctgtttc
     2281 tttttaccct taacaacgat ttcgttttca ggcttgccag ataataaatt tgccaatgcc
     2341 tcgagtatga gtaatttttt tcgcaccttg tcaggatcag ttggtacgtc gttgacaatg
     2401 acactgtggg gacgccgcga atcgttacac catagtcagt tgacagcaac catcgatcaa
     2461 tttaaccccg tgtttaattc atcgtcacaa attatggata aatactatgg ttcgctttca
     2521 ggagttctta atgaaattaa taatgaaata actcagcagt cactttctat ttctgcaaat
     2581 gagattttcc gtatggcggc tattgctttt atcttactta cggttttggt ttggtttgcg
     2641 aaaccgccgt ttacagcgaa aggcgttggg tgatggaaaa agaggaggct atagcaggta
     2701 aaacggcaga atcttaagtc tgcgtcatcg gcagcatcgc tcacccaggg aaaggatggt
     2761 gatgctgcgt tgaaacgtta ttaacggcct tttgccagat attgattcat ctcttcttcc
     2821 ggcaccattc cacctcccgt cgcccacacc agatgagtgg cattacgcag ttgttctgcg
     2881 ctgaaaccgt gcatctgttg gtaacttact gatgcgcaca cgcgctgagg tccggccata
     2941 cccgccagtg ccgaaggttc aagacgaata ccttcttcct gcgccagcca gccaagcatg
     3001 tcatacatgg tttgatcgct aagggtatag aagccatcca gcagacgctc catcgcccgc
     3061 ccgacaaagc ccgatgcgcg accaaccgca aggccatcgg cagcggtaag gttgtcgatg
     3121 ccaatatcct gaacagaaat ctgatcgtgt aatccggtat ggacgcctaa taacatacac
     3181 ggggagtgtg ttggttcggc aaaaaagcag tgaacatgat cgccaaacgc cagtttaagt
     3241 ccgaatgcga cgccaccagg accaccgcca acaccacacg gcagatagac aaatagaggg
     3301 ttatcagcat cgacgatacg gccttgctgg gcaaattgcg ctttaagacg ctggccagcg
     3361 acggaatacc caaggaacaa cgtgcgggaa ttctcgtcat caataaagaa acagttcggg
     3421 tcagactgcg atgctttacg tccttcctcg acagcaacac cataatcttg ctcatattcc
     3481 acgaccgtaa cgccatggct gcgcagtttc gcttttttcc atgcccgggc atcagcagac
     3541 atatgcactg tcaccttaaa gccaatgcgg gcgctcataa taccgattga taaccccaga
     3601 tttccggttg agcccacagc aatgctgtat tggctaaaga actgtttaaa ctccggagaa
     3661 agcagtttgc tgtagtcatc ttcaagcgtc agcaaccctg cttccagagc cagtttttct
     3721 gcatgtgcca ggacttcata aatcccgccg cgtgctttta tggagccgga aataggcaaa
     3781 tggctatctt ttttcagtaa cagttgcccg ctgatcggtt gctgatattc cttttccagc
     3841 cgtttttgca tagccggaat ggcaaccagt tctgattcaa taatcccccc agtggcagca
     3901 gtttcaggaa atgcttttgc cagatagggg gcaaaacgag ataagcgcgc atgggcgtcc
     3961 tgaacatcct gttcggtcag gccaacataa ggtaaacctt cagccaatga ggtcgtgcca
     4021 ggattgaacc aagtggtttc tttaagagca accagatcct ttaccaacgg atactgggcg
     4081 atgagcgaat tcattttagc gttttccata gtacgtctct ttgcgcttag atgataaatg
     4141 acagcaggaa tgtgcccgcc agagcgatga ccgaagcgat aaatgtcgct gtcgtatagt
     4201 atttaaatgt ttcattgagc gtagcgccgc aatattgctt caccagccag aaaagcgagt
     4261 ccgtaacgat cgtgcagcca atcgcaccgg aaccgatagc aatcgcaata atttccgggc
     4321 tgatgtcggg atacagcggc agcatggggg caacaattgc cgttgccccc atcattgcca
     4381 ccgtagcgga gcccactgca gcatgcagaa taagtgccac caaccaggcc agaagaatcg
     4441 ggtgcatatg catattggag agaataactg ccagcgtatc agcgagactg ctgcttttta
     4501 aaatggcgtt gaatgcgcct ccggccccga taatcagcaa aatattagca atagaaccga
     4561 aaccattttc cgtatgtgtg agcatcgtcc ccatgctcat atgctgacgt atacccaaca
     4621 cataataggc gacaaacacg gcgataaaca tggcagtgat agggttgcca ataaactcaa
     4681 gcaaggtata caaaccactc tcacgcgcca tattcaattc ggcaatcgtt ttaaccaaca
     4741 tcagcgcaat gggtagcagt atggtgaata acgttgcgcc taatgaaggt agtgtttttt
     4801 catcgcgaac tttgagatcg gcaaactctg tgggtacagg tttaaagggc agtcgttgcc
     4861 ccagaaattt aaggaaaagt gggccaccga tcagtgatgc catcagccca accagcaaac
     4921 cgtagacgat caccgaaccg atatctgcgc ccagcttatt ggcaacatat aaagcagccg
     4981 gatgtggggg aaccacgcag tgcacagcca tcaatgcggt acacagcgga atggccagct
     5041 ttaacaatga ggtattggtt tttttggcaa ttgaaaaagc cagaggaatc aatagcacga
     5101 cgcccacttc aacaaacagc gtgatgccgc aaatcaggcc aaccagcacc ataatgacat
     5161 cagccgaaag ccaccggcag cgttgaagtg tcagaccaat tctttctgcg gccccggata
     5221 cttccatcat ttttcccagt atcgtgccaa ggccgataac cgcagcgagg aaccccagcg
     5281 ttccgccaat tccactttca atagcattta ccatatccag tggccccata cccatcatcg
     5341 ttcccacgaa gaagctggcc agcaacagcg ccagaaacgg gtggaatttg aacttcacga
     5401 tggtcagtac aattaacacg atgctgataa gcagcgtgct cacaacccag atttgagagt
     5461 gcatatctca ccttcccctg tgattaacct gatgatattg gacaaaaaaa caacgcaggc
     5521 tgacaaacga taaaatttgc ctttcacatg agctaaattg catcaaggga gtgatttaca
     5581 tctaaaaatg aaggtgaatt gagatatggt tcactttagc tcaccttaca gctgaaattt
     5641 tgccgctatt tttttacact taatagaaaa atgaggtgat tatggaaccc cttcgtgaaa
     5701 taagaaatcg actgcttaac ggctggcaac tatcaaaact gcatactttt gaagtggctg
     5761 ccaggcatca gtctttcgcc ctggcggcag aggaattgtc gctgagcccc agtgcggtaa
     5821 gtcaccgtat caatcagctg gaagaagaat taggtattca gttgtttgtt cgttcccatc
     5881 gcaaagtgga attaacgcac gaggggaaac gcgtttattg ggcgctaaaa tcgtcgctgg
     5941 ataccctgaa tcaggaaatt ctggatatca aaaatcagga gttatcggga acgttaactc
     6001 tgtattcccg gccctctatc gcccaatgct ggttggtgcc cgcattaggt gactttacac
     6061 gccgatatcc gtctatttcg ctcaccgtgc tcactggtaa tgacaatgtc aatttgcaac
     6121 gtgccggaat cgatttggcg atctactttg atgatgcgcc gtcagcgcaa ctgacccata
     6181 actttctgat ggatgaagaa atcttgccag tttgtagccc ggagtacgct catagacatg
     6241 atttaaccaa cacggtaatt aacctgcgtc actgtacgtt gctccatgac agacaggcat
     6301 ggagcaacga ctccggtacg gatgaatggc atagttgggc gcaacattat gcggttaatt
     6361 tgccgacatc ttctggaatt ggctttgatc gttctgattt agccgttatc gccgcgatga
     6421 atcatgttgg ggtggcgatg ggaaggaaac gcctggtaca aaaaaggctt gccagtggtg
     6481 agctcgtcgc gccgtttggc gatatgacgg tgaaatgcca tcagcattat tacatcacca
     6541 cattaccggg caggcagtgg ccaaaaattg aggcatttat tacttggtta agagaacagg
     6601 taaaaaccac ttcataatga ataagaccac gagttcctgg tgtgccttat tcggcactgc
     6661 tagtgcgaat ttaataattt acggttttta cgtggtttta gggataaaca c
//
LOCUS       DAPQZD010000225         6583 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPQZD010000225 DAPQZD010000000
VERSION     DAPQZD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.227"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(31..636)
                     /locus_tag="RO454_004570"
     CDS             complement(31..636)
                     /locus_tag="RO454_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0936293.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETG
                     SGTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMN
                     GMAFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSD
                     RLLPKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
     gene            731..3628
                     /locus_tag="RO454_004571"
     CDS             731..3628
                     /locus_tag="RO454_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="HDY0936294.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRR
                     PENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQ
                     HMDTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVS
                     TIERLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGN
                     NSAAANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWA
                     ILAVCVVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALG
                     ASLLEARSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRY
                     APRMLRCLKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDT
                     RLWEVAVLFHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLA
                     DRKARLTIALKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPS
                     VRITDMLLEVDAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTH
                     DYWQLSRLARWHVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGE
                     AMNMVNAKYGSVPGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVRE
                     QYADTAGFTDHLFGASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKARED
                     LIVANWPDIFRCAATMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEW
                     ILDTDMQRRAQIGLNKGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVII
                     YWNTVHLGHAVTERRNEGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
     gene            complement(3765..4166)
                     /gene="pemK"
                     /locus_tag="RO454_004572"
     CDS             complement(3765..4166)
                     /gene="pemK"
                     /locus_tag="RO454_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HDY0936295.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(4099..4356)
                     /gene="pemI"
                     /locus_tag="RO454_004573"
     CDS             complement(4099..4356)
                     /gene="pemI"
                     /locus_tag="RO454_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HDY0936296.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(4449..5102)
                     /locus_tag="RO454_004574"
     CDS             complement(4449..5102)
                     /locus_tag="RO454_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDY0936297.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(6041..>6583)
                     /locus_tag="RO454_004575"
     CDS             complement(6041..>6583)
                     /locus_tag="RO454_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDY0936298.1"
                     /translation="ESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDIT
                     FTSALFAALDVSEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRS
                     YQTELKSRGIKRARARRDANRERQDIVTLVKRQLTREISEGRFTVNREAVKREVERRV
                     KERMILSRNRNYSRLATASP"
BASE COUNT         1505 a         1810 c         1753 g         1515 t
ORIGIN      
        1 gtcggttccg gctgagggcg aaatgacacc ctaagcgtta gctctgtgtc gttgcacgat
       61 gtcagcgacg gtattcttgc tgataccgag ctcgcgtgcg atccagcgat agctgcgtcc
      121 ctcggccctc atcgcaacca ccttaggcaa aagtcggtct gattttggtc gcactccggc
      181 ctgacgacca agcctcttac cacgtgcctt cgcaacagca aggcctgact tgacccgctc
      241 gctgatgaga tcccgctcaa actccgcaat gccggaaaga aacgtcgcca gcattcgtcc
      301 atacggcgac gaaagatcga acgccattcc attcatggct atcacggaaa ccttccagtt
      361 ctccagttca cgtagcgtat tgagcagatc gagcgtcgag cgcccccacc gggaaagctc
      421 agtgaccagg attgcatcaa tttgtctgga ctgggcaagc gccaggactt tctttcgctc
      481 ggcccggtcg agtttagttc ctgaacctgt ttccttaaat attcccacca cgtcgtagcc
      541 ggcacagccg gcgaaggctc gcagatcaaa ttcctggcgt tcacaagact gatccgctgt
      601 tgaaacccgg cagtaaatgg cggcacgatg tcccaattga accctcctgg atttttgtat
      661 cggaacgccc tgatttatat gggctggctg ttgtccaaaa cagactatac ttcaaaaggg
      721 acgaatttgt atgtcacgac gccatatttt caccgaacgg cagcgagcag cgctgttcga
      781 tctgcccacg gacgaactgt cgctactgaa gttctacacg ctgggcgatg atgacctgga
      841 aaacattagg cagcgccgca gaccggaaaa caggattggc tttgccctgc aactttgtgc
      901 cttacgatat ccgggccgtg cactggctcc tggtgagatg atcccgcgtg aagtcctttc
      961 cttcgtcggt gctcagcttg gagttccggc tgatgcgctt ctcacttatg ccacacggcg
     1021 ccaaacccgt cagcagcaca tggacacgct gcgcgaaatt tacggctaca agaccttcac
     1081 gggccgtggt gcccgtgatc tgcgggagtg gactttcggc caggccgaag atgccagatc
     1141 aaacgaggat cttgctcatc gttttattgt gcggtgtcgg gaaacttcca ccattctgcc
     1201 cgcagtatcg acaatcgagc gcttgtgcgc ggatgctctg gtcgccgctg agcggcggat
     1261 tgaaacgcgg attgcggaaa atttaacagc ggatgttcgc gatcacctgg acaaacttct
     1321 gagtgaaatg ctcgccggca atatcagtcg tttcatctgg cttcgcaact tcgaggttgg
     1381 taacaactcg gctgctgcta accgtttgct cgacaggctc gaatttctgc gtaccctgaa
     1441 tatcaatcat agtgctttgg ccagcatacc tgcccatcgc attgcccggc tgcgtcggca
     1501 gggtgaacgc tacttcaccg acggtttgcg tgacatcact tcggaccgcc gctgggcgat
     1561 ccttgccgtc tgtgttgtgg agtgggaagc ggcgattgct gatgccatag tcgaaaccca
     1621 tgacaggatc gtaggaaaaa cctggcggga agcgaagcgc cagcatgacg aaacaatttc
     1681 cggctctaaa gccacactca cggatacgat ccgtaccttc accgcgctgg gagcttcgtt
     1741 gcttgaggcc cgcagtgacg gaaccccgct ggagatggct gtcgccagtt cggttgcatg
     1801 ggaccggctc gctcaactgg tagcgacagg gactcaactc agcaacacgc tagccgatga
     1861 gcctcttgca tatgtcgggc agggatacca tcgctttcgt cgttatgcgc cccgcatgtt
     1921 gcgctgtctg aagctcgaag ccgcgccggt cgccggacca ttggtagcag cagctttgtc
     1981 gatcggagag atgaaaggtg ttgcatcgcc agaaaggcgt ttcctgcggc ccagctccaa
     2041 atggaaccgt catttacgag ctcaggaaaa aggagatacc cgtctttggg aagtggcggt
     2101 actctttcac ctccgggatg cttttcgttc cggagatgtc tggctcgctc attcgcgccg
     2161 ctatggtgac ctcaagcagg tactggtgcc gatgatcgcg gcgcaggaaa atgcaaaact
     2221 ggccgtgcct tccaacccac aggattggct ggcagacaga aaggcgcgac tcacgatcgc
     2281 tcttaagcgg ctggcccggg ctgcccgtaa cggcactatt ccgcacggta gcatagaaga
     2341 tggaacgttg cggatcgaca ggttgacagc agacgtgccg gatggtgccg aggcactcat
     2401 actggatctg tatcgccgaa tgccgtccgt tcggattacc gacatgctgc ttgaagttga
     2461 tgcagccctt ggtttcacag atgcgtttac ccatctgaga accggggctc catgtcgcga
     2521 ccggatcggt ctgctcaacg tcctgctcgc tgaagggctc aatctgggcc tgcgtaagat
     2581 ggcggaagct acaaacacgc atgattactg gcagctctca cgccttgccc gctggcatgt
     2641 tgaaagcgaa gccatgaacc aggcattggc aattgtggtg gccgcgcagg gtaaactgcc
     2701 gatgtcacgc gtctggggga tgggcacgtc agcatcgagc gatggtcagt ttttcccgac
     2761 agcgcggcat ggcgaagcca tgaacatggt caatgccaaa tatggttctg ttcccggcct
     2821 caaagcgtat actcacgtaa gcgaccagtt cgcgccattc gcttgtcagt cgatcccggc
     2881 gaccgtgagc gaggcaccgt atattctcga tggactactg atgaacgagg tcggtcgcca
     2941 tgttcgcgaa cagtatgccg atacagcagg attcaccgac catttgttcg gagccagtag
     3001 cctgctcggc tacaatctcg ttctgcgaat cagggatctg ccatcgaagc ggttgtacgt
     3061 atttaatccc gatacgaccc ccagggagtt acgcaagttg gtaggtggaa aagcccggga
     3121 ggatcttatc gttgcgaact ggcctgatat tttccgttgt gccgcgacga tgaccgctgg
     3181 caaaatcagg cccagccaac tcctgcgcaa gctcgcttct tacccacgac aaaacaacct
     3241 tgcagttgcg cttcgtgaag ttggtcgtat tgaacggacc cttttcatta ttgagtggat
     3301 cctggatacg gacatgcagc ggcgtgctca gatcggtctt aacaagggag aggcccacca
     3361 tgcgctcaaa aatgcgctcc gtatcgggag gcagggggaa attcgcgatc gcacgacaga
     3421 ggggcagcac taccgaatcg ctgggctcaa tttattgact gcggtgatca tttactggaa
     3481 taccgtccat cttggtcatg ccgtcacgga gcggcggaac gaagggttgg atgttccccc
     3541 tgaatttctt ccccacatat ccccattggg ctgggcgcac attctactga ctggcgaata
     3601 tctttggccc aaggaaccga aagcttaggg tgtcatttcg ccctcagccg gaaccgaccc
     3661 cttttagcca aataacgttt gggaatcacc agaatggtgg gacaacagcg gttttagtgc
     3721 cctaaatcgt acgttttcat ccagttgccc ctcaaacccc atgttcaagt cagaatagtg
     3781 gacaggcggc caagaacttc gttcatgata gtctccggaa cccgttcgag tcgttttccg
     3841 ccccgtgctt tcatatcaat tgtccggggt tgatcgcaac gtacaacacc tgtggtacgt
     3901 atgccaacac catccaacga caccgcaaag ccggcagtgc gggcaaaatt gcctccgctg
     3961 gttacgggca caacaacagg caggcgggtc acgcgattaa aggccgccgg tgtgacaatc
     4021 agcaccggcc gcgttccctg ctgctcatga cctgcggtag gatcaagcga gacaagccag
     4081 atttcccctc tttccatgtc agatttcctc ctgaccagtc gccggtgcat ccagccattc
     4141 tcgttcttca gctgatattt cagcattcgg atcacactgt gccagtagct cagccagtga
     4201 atattgcggg cgtctgtacg gctcaacaat cagccggcca ttatcaatga ccatgccaac
     4261 ttcattatct gtgcccagag acagcgcatt cagcagtgcc ggtgggacgg tcagcataac
     4321 tgagccgcca accctcttca gtcgggtggt atgcattctt cacctccata aaagttatat
     4381 ttaaatataa catccactaa aaaaacacac caggctttaa cgcacaatgt ttaataaaaa
     4441 tataactttc aaccaaacag taaacccagc gtggttgctt ccatatgcag caataccggc
     4501 agcagcaggc caccacttct gatcctggcc actgatgtaa tcaaccccac caggaacagt
     4561 tctgccagtg tcagcaggtt ctgatactgg ctgtgcgcgg cgacgaacaa caacgacgtt
     4621 atcagcgccc ccagccacat cgtccagcag taccgtgaac ggaagacgtt cagcataatc
     4681 ccccggaaca gcgtttcctc attcaacggg gcaaggataa agatggtcag caacgtcagg
     4741 atcacgtcag gtatggactt atcggcaaaa agtttcgtca taaatggctc agcaggcaga
     4801 gccagcgcct taccgagcag aaatacaccg acatacacca cggccatcgc accgaccagc
     4861 cacggtacgc caacgttgcg cagctgacca acgaccggta gcggagctat ccaacggcgg
     4921 tataccagga aaacacacag caggtacatc agaacagtac catgactgaa gaacaaatag
     4981 ttttttcctg atccataaag cagaacggcc tgctccatga caaatctggc tccccaacta
     5041 atgccccatg cagccagcat aaccagcata aactgcagat attgattacg tgtttgaatc
     5101 attgcatcgc ctgtaaattt ttaacttgtc ctatttttgt cattaccacg tatatacaca
     5161 tgtataacaa ttcagatatc gttaccagga tatgccgcat cagcggcatg gaaggcggca
     5221 ctctgttgtt tcatatgata caggagtaaa accgccgaag cccggcgtaa gccggtactg
     5281 attgatagat ttcaccttac ccatccccag ccctgccaga ccatacccgc tttcagccat
     5341 gagagagctt ctgtgcgcgg tcggagtggt cccgacgagg gtttacccga agtcggggcg
     5401 tatctccgcg ttagcgggcc gtgagggccg cttacgagcg tgtactgaga acttccagcg
     5461 agaagactga cagcgatgaa gatgtagtta caacattcat aattaaaagc gactctgttc
     5521 cggccctttg ggccggggcg gggccgcttt tcagttatga gggaggggct ttgtggtttc
     5581 ggttctgcgc tggaccgggg tttttctgga ggttgttttt gtgtgttgta actaaagtgg
     5641 ctccggtcgg ggcccgccgc ttgcggtggg aggtgcatat ctgtctgtcc ataggacaag
     5701 cagtgaatag gttttctttt taaatgaatg taattaagta gtttaaagga gatataaaca
     5761 ggtgtttaaa agatacattg caccctgtag ggctgacggc tggcgcttta tgacattaac
     5821 gattgtaacc ttatggggaa gtcccttgca gttaaatgtg gataagcaaa attcctcgtc
     5881 tgtgaggcgt gttttgtatc aaaaacagga gggaccggat gcacctgaag gtggatgatt
     5941 aggttgtttt ttgtatgcag tgctgttttt ttgttcgccg acaggcttca ggcgtgcaga
     6001 tgcctccggt gcgggccgga ttattcagag gaggtcactt tcaaggggaa gctgtggcca
     6061 gccggctgta attacggtta cgtgacagaa tcatgcgctc tttcacacga cgctccactt
     6121 cgcgttttac cgcctcacga ttgacagtga agcgcccttc cgagatttca cgcgtcagct
     6181 gccgtttcac cagggtgacg atatcctgac gttccctgtt cgcatcacga cgcgcacggg
     6241 cacgctttat tccccgggac ttaagctctg tctggtaact gcggaaacgc tcacggacaa
     6301 aacgccaggc tttcgctatc agttcatcca tacccagggt atccagcccc tgctttttgc
     6361 gctgtctgtt ttcccattcc acacggctgc ggcgcgcggc ggccactgcc tcctctgata
     6421 catcgagggc agcaaacagt gcagatgtga acgtgatatc ggtcggaatg tagcacccga
     6481 taagcgggtc atattccgtc tggtaggtaa tcagtcccag ctctgacagg aacgtcagag
     6541 cccgggtggc acgggtgatg gagagttttc ctgcaccgga ctc
//
LOCUS       DAPQZD010000226         6492 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPQZD010000226 DAPQZD010000000
VERSION     DAPQZD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.228"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(74..346)
                     /locus_tag="RO454_004576"
     CDS             complement(74..346)
                     /locus_tag="RO454_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDY0936299.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNG"
     gene            complement(629..3316)
                     /locus_tag="RO454_004577"
     CDS             complement(629..3316)
                     /locus_tag="RO454_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDY0936300.1"
                     /translation="MKTPSVSTISGAAIGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEVAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKSLHGYSLPLLSQPLNLAGITFSTGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSEGWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKTLAEHLEQHVAH
                     YHGAPFRDWLYCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQSCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(3313..3723)
                     /locus_tag="RO454_004578"
     CDS             complement(3313..3723)
                     /locus_tag="RO454_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936301.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMVRVHNEPVYIEDGER
                     IELSHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(3716..3952)
                     /locus_tag="RO454_004579"
     CDS             complement(3716..3952)
                     /locus_tag="RO454_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936302.1"
                     /translation="MKSMNFKQGCILSEQGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGVMRDE"
     gene            complement(3949..4539)
                     /locus_tag="RO454_004580"
     CDS             complement(3949..4539)
                     /locus_tag="RO454_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDY0936303.1"
                     /translation="MPALINPGNDQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCYL
                     KAILHALGVFFCVAALTHLKFMVWWLIVFFYFLRHIIRIMVVQAGQLSGWPVSFRAGI
                     PTPVWATTHERRNSGGSVTRYLKEVAIMATVPALSHPEFTFVFLAVRRTDRDARPRPV
                     RVIAECEHAARLKLATEFILSFAARIPVKRAGEVVA"
     gene            complement(4627..5631)
                     /locus_tag="RO454_004581"
     CDS             complement(4627..5631)
                     /locus_tag="RO454_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDY0936304.1"
                     /translation="MYAFKTERPDAARRYHNKFVTGTNYQEESNRRFALSEPTENILR
                     TSILESGWLIKNITLRDVNAVSGNAVNIGASELYTGRVDGGRFHKKMAINGTEFFLTE
                     TDTCARISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEI
                     NKKGEDVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDALANHLITELIAEQFRE
                     DPRLVVLVGAELAAHQRLKLFNAADRPSDVNAAQMATSSVAGRFAFIPPFMPGKRLAV
                     TTLDNLHIYTQAITRWFRAEFDDESSEYVHSYLRNEGYALGEPELYAAVDECTLTFAD
                     "
     gene            complement(5654..6487)
                     /locus_tag="RO454_004582"
     CDS             complement(5654..6487)
                     /locus_tag="RO454_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936305.1"
                     /translation="MASIKNEHVSVLDSYKVRSFKETHSDTACIVRIIEIYSLNKLRA
                     KGEKLYSLTGLTVPDTETVANEINLLLSRYAQLCRQEEEELSFRQREVTNAEVAWKST
                     FSKNGVSSIAEAKTNKMGHAERADAERYYHLAVSRLNEQHSRLSTIKLLPGVLADEGN
                     YIGKGIDKRLLNIFPQSGQIPADFISVFNDSDVVRDIKFITDALKSLSDSVSEIISRC
                     SVPTDRYVLNNGGMARAMAYREYYRADNYVLRSVVSDRDYVEHVMKYNLVTEYKNKIF
                     S"
BASE COUNT         1604 a         1804 c         1374 g         1710 t
ORIGIN      
        1 aattttatga ccgtataaac tgaaatcctg tgttcaggtt ccgggaacag atcggccatt
       61 cccgacgcat actttaccca ttcttttgcg cagccagcag caactttaaa gccttttccc
      121 gttcctcggg tggaattgca tcaatcagct ctttaaccct tccctgcccg ttcagcgcgc
      181 ttggactaac ggaatgggag aaagcaacat tcatcacaaa cgagtgaccg cactccacat
      241 tagagcaata gcagtacaca tcgtacagtt gtgggtgttt cctgtttgtt ttacctatcc
      301 gtgctactgc ctgacatgtg gggcagtaca ttctcatcat cttcatcgtt ctgtgcccta
      361 tactgttgcc cgccagaaga agggatttgg atccctacct cttgttctga acgtgtaaca
      421 ctggtaacac gtgtaacgct attgatttaa aaggatttaa tctgttacca gttagcaaaa
      481 tcaagaggta acgcgtggta acacaccatt ttttgttacc tctgttacca gtcagtattt
      541 ttgactggta acactgaaga accgcatcag taacgggtgt tacctctgtt accagtgtta
      601 cctcataaaa ataagactca cgcgataatc actcaggttc agcgcttccc agtacctgtg
      661 tattgaactg gtacacacgt ttaagcccta tttctggcag gcgaatgctg ttttgcgtcc
      721 ggccatcttc accgggtttc agccagcccg cgtccacaca caacctggcc accttacgcg
      781 agtcaaatcc cttacagatc tccttccagc ctgacgggag aacgtagaac gtcacaaccg
      841 gctctgtcac gttgtctcct ttatcgactt ttctgaatcc catcattgaa acaggccggt
      901 tcctgtcgtc attccagtca gcaaaccgac tgaactgatt acgcgtcatg aagtcccgaa
      961 cctgttcaag tgccgcttta tcttcctgat tagctgtatg gccccggtca gccatccatg
     1021 cagccagaca gctttgagcg gcacgaaagg cctctccctc cggccatcct gtgatgcccg
     1081 cttttgtggc cagctccccg gccatcgcaa ccagagcaaa acgggtaaca gcccggccaa
     1141 cctgattccc ggcattttcc ggcgtcagcc ttcgggtata ctctttcaat aaggctttcg
     1201 cctgactggt cagttccggc agatcagcgg tcaggcaata cagccagtcg cggaatggtg
     1261 caccatgata atgcgctacg tgctgctcaa gatgctcagc cagggttttc ccgctactga
     1321 agccatgaag ttcttcaaac acgccatact tgcccgaatc gcttgggatc tggatcattc
     1381 tgacctcaac cccggcatac gtacgttccc cggcgcttgc cgcatgctct accagtgaca
     1441 gttccccggt agacagaaac aacagattcc agcggttggt ttcccttacc gaaccatctg
     1501 ttctggctct ggctttgccc tgcccgttag ccagcatata agcgatattt cccgcctccc
     1561 tgccgtcaac ctcccggatt tcatccagca tcagcgtggc atcattgcgg cggctcgccg
     1621 ttccttctaa ggcattcccc gtcgcccgcc acgtatgcca gaaatctgtt ccaccacata
     1681 ctgatgcagc cactttcatc gtcgtggttt taccgtctgt ggattcccct ttgaggtgat
     1741 aacctccacc gccgatacca accagtttca gaaggggggc agcaaatgcc agactcaccg
     1801 caaaggcaag acgggcattc tttatgcaat aacggcctat attttcacgc cagccctctg
     1861 atgtgccgct gacacgaaaa tcacgcccct gcacactcga tgtttgtaag atgactgact
     1921 gggcttcgcg ccctatcact tcatcctgga gaacgtaaac cccgccgtgc cagccagttt
     1981 tattcacaca ggttactttt ctgtccggtt tacacagcga gatatattcc atcaggaatg
     2041 ctctggccat gccgttaatg ttgatgtaag aaagcccgtt aaccagcaga acgcggcgca
     2101 gttcttcccc gctaccgccc agcatctcca tcggcattgc ccatttacgg ctgtttccgt
     2161 tggtatcttc ccattccagc aaacgcccgt aattacttcc atcagcatca ctggtgattg
     2221 ccgtcacccg caacggactg caaattttga tattacgtat ctctgtatca ccatcagact
     2281 tattaaccag cttgtcgtac cacaaatatt cttttgtcag ccggaaccct tctggtaaac
     2341 gggtctgccc tttgccatgc agcgtcattt cttcacgaaa ggcatctctc gcccgttctt
     2401 tccccacttc ctgccgataa tcatcccagt cagccttatg gcgtaccgga gggagcgtta
     2461 cccagccatc caccgctttg gcggctttct ctgcctgaat acgcccggtg ttttctttgc
     2521 cgtcagccag atcattatct ccggccagaa tgatccgggt ttccggccag cgttttcgga
     2581 tctgctccgt caccttcagt aaattggtgg cagcgacagc cgccacgatc catccttcag
     2641 tcagcagact gacggtaagc gccgtggcaa aaccctcagt gataatcacc tgttcaggtg
     2701 tttcagcggg tatatcggtc agggcaatga atgcgccaga cagctggcta ccgggcagca
     2761 gacttttatc cccgtcagga ttgataagct ggccaccagt aatattcccg gaaatatccg
     2821 tcaaaggcag cagtaatgaa ccggtggaaa atgttattcc ggccagattc aaaggctgtg
     2881 acagcaaggg aagtgaatac ccgtgtaagc tcttatttgt cagatagacc ggctcaccat
     2941 tgcaggactg ctgtctgagt ctggtatatc gctccgcgcc cgcttctttt cctgcggcct
     3001 ttttcctggc tggcaatgcg ggcttctcct gtatttctgg taatgcaagc gcctcagata
     3061 ccatcccggc gacttctttt atctttcttc ctgtcaccag acgaaccaga tcaagaccat
     3121 cgccatgacc gcactgatta caaaaccacg tccctcgccc cgctttatcg tccagtcgaa
     3181 aacgatcttt tccaccacag gcagggcaag ccccatgatg tcctgcggac ggtactttaa
     3241 tacctaatgc tgacaggata taaggccagc gcccaattgc agctccggaa atcgtggata
     3301 cagacggtgt tttcacagca gcacctcctg accttccggc ttcacaaatt caaaaccttc
     3361 gggtagttcg ccatcaggtg ctccgaaaat aatatcccgg taaacctcac gcatctctgt
     3421 gacaccaaaa actgacaggc gaatattttc tgacataaag cctggtttaa gcattttttc
     3481 cagtttctct gcggccagta tgcaaccagc atcagcccca aactcattca cccacggcat
     3541 ttcaatatgg taaataatat tactcaccac tttttcgtgg gagagttcta ttcgttcgcc
     3601 atcttcaata taaacaggtt cattatgaac acgcaccatt aaggaaatga atgcatcagc
     3661 aatgtagtag cgtaagactg ctgttcgtaa agcatgcgcc tttaaatttt gattattatt
     3721 catcgcgcat cactccctta attgtgctgg tttccttaac aacgccatcc agttgttcgg
     3781 caatacaggc cactaaagcc gcagcgcctt ccgctgaaac agttctcgct ccattcactg
     3841 tgggcacgga caagacctct gcaagaaact cactggccag tgccgcccgt aacagacgac
     3901 tctccccctg ttcactcaat atgcagcctt gcttaaaatt catggatttc atgcaaccac
     3961 ctccccggct cttttcacag ggatacgggc agcaaagctc aggataaatt cagtggcaag
     4021 tttaaggcgt gcagcatgct cgcattctgc gattacacgc acagggcgtg gacgggcatc
     4081 acggtctgta cggcggacag ccagaaagac aaaggtaaat tcagggtgag ataaagcagg
     4141 gactgtagcc ataatggcaa cctccttcag atagcgggta acgctaccac cggagttcct
     4201 acgctcatgg gtggtagccc agacgggggt aggaataccg gctctgaagg ataccggcca
     4261 gcccgaaagc tgccccgcct ggactaccat aattctgatg atgtggcgta aaaaataaaa
     4321 aaatacaatc agccaccaca ccataaattt taggtgagtc aaagctgcga cacaaaaaaa
     4381 cacgcctagc gcgtgtagta tcgccttcag ataacacggg ttcctacgcc cggctgccga
     4441 ttttgcggca acctttaaac tatatcccgc gcattcctgg tgaggcaaga aatttataac
     4501 cgaaaactga tcattgcctg gattaattaa cgctggcatg cttaagcctc tgcttaaaaa
     4561 tagttcttca tttttcctga tgctgtaaac gagacccgcc tctgagcggg tctttctgtc
     4621 gctaagtcaa tccgcaaacg tcagggtgca ctcatcaaca gccgcgtaca gttcaggttc
     4681 accgagtgca taaccttcat tgcgcaggta ggaatgcacg tattcactgc tttcatcatc
     4741 aaactcagca cgaaaccagc gtgttatcgc ctgagtgtaa atatgcagat tatccagcgt
     4801 cgttactgcc agacgcttac ccggcataaa cgggggaata aacgcaaagc ggccagcaac
     4861 agaacttgta gccatctgtg ccgcattcac atcagacggg cggtctgcgg cattaaataa
     4921 cttcaggcgt tgatgtgctg ccagctctgc cccaaccagt acaaccagac gaggatcttc
     4981 acgaaactgc tcagcaataa gctctgtaat cagatggttg gccagagcat caatattttt
     5041 ccaggtgcca gtctctccca gagtgacagg ctcagagatg atttgctttc cgttcccgta
     5101 ggctttcgcc agtgcatgcc agccaatatt gacatcctcg ccttttttat tgatttccgg
     5161 gtttgtagta tttgcaatcg acacaccgtt gaaaccgacc cgcagcatgt ccagcgcata
     5221 tgcctgggca aaaaagctct caaccgtttt ctcaaactca tccggcacgc cggtatggta
     5281 aatatgagac atatcggaat agctgatccg ggcgcaggta tccgtctctg tcagaaagaa
     5341 ctccgtacca ttgattgcca tttttttgtg gaaacggcca ccatccacac gtccggtata
     5401 cagttcactg gcaccaatat tgacggcatt acctgacacg gcgttaacat cacgtagggt
     5461 aatattttta ataagccacc cggattccag aattgaggta cgcagtatat tttcagtagg
     5521 ttcgcttaat gcaaagcgtc tgtttgattc ttcctgatag ttagtgccag tgacgaattt
     5581 attatgataa cgacgtgctg cgtccggcct ttcagttttg aaggcataca tagtttttca
     5641 cctttttaag tatttaagaa aagattttat ttttgtactc agtaaccaga ttgtatttca
     5701 ttacatgctc aacataatcc cggtcactta cgactgaacg taatacataa ttatctgctc
     5761 gatagtactc cctgtaagcc atagctcttg ccattccacc gttatttaat acataacgat
     5821 cagttggaac gctacaacga ctaataattt cactcacgga atcagataaa gacttgagtg
     5881 catcagtaat aaatttaata tcacggacaa cgtcactgtc attaaataca gaaataaaat
     5941 cagcagggat ctggccggat tgaggaaata tattcagtaa acgcttatcg attcccttcc
     6001 caatgtagtt tccttcatcg gccagtactc ccggcaaaag cttgatcgtg ctcaatcgac
     6061 tatgttgttc attcaaccgg gaaacagcca gatggtaata ccgttcagca tccgctcgtt
     6121 cagcatgccc cattttattc gttttggctt cagcaatgct actgacgccg ttttttgaga
     6181 atgtgctttt ccatgcaact tccgcatttg tcacttcacg ctgccggaaa gataactctt
     6241 cctcttcctg acgacacaac tgggcataac ggctcaggag caggttgatc tcattcgcca
     6301 cagtttccgt atcaggtact gttaaccccg taagtgaata cagcttttca cctttagctc
     6361 gcagcttatt cagtgaatat atttcaataa tacgcacaat acaggccgtg tcgctatgag
     6421 tctctttaaa tgatcttact ttgtaactat ctaacaccga cacatgttcg ttcttaattg
     6481 atgccaattt ct
//
LOCUS       DAPQZD010000227         6453 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPQZD010000227 DAPQZD010000000
VERSION     DAPQZD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.229"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            155..463
                     /locus_tag="RO454_004583"
     CDS             155..463
                     /locus_tag="RO454_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936306.1"
                     /translation="MNDVQNRRILALLNMLYYALGNRTTCDICHKYFILVLSVMNNLT
                     INYGSQTSPELTGISYEDANDLITELILFIEDVNADNDMEYPAHLNKIQALLNFAVAN
                     "
     gene            complement(520..1110)
                     /gene="cobO"
                     /locus_tag="RO454_004584"
     CDS             complement(520..1110)
                     /gene="cobO"
                     /locus_tag="RO454_004584"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDY0936307.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1107..1865)
                     /locus_tag="RO454_004585"
     CDS             complement(1107..1865)
                     /locus_tag="RO454_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDY0936308.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2085..3134
                     /gene="sohB"
                     /locus_tag="RO454_004586"
     CDS             2085..3134
                     /gene="sohB"
                     /locus_tag="RO454_004586"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDY0936309.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3170..3421)
                     /locus_tag="RO454_004587"
     CDS             complement(3170..3421)
                     /locus_tag="RO454_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDY0936310.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3801..6398
                     /gene="topA"
                     /locus_tag="RO454_004588"
     CDS             3801..6398
                     /gene="topA"
                     /locus_tag="RO454_004588"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDY0936311.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1675 a         1506 c         1697 g         1575 t
ORIGIN      
        1 tctacaagat ttatactcta ataaatctct ttcaaaagcg gatttagagc gtagaaatga
       61 gatccttcgt tctcaagtta ttagtggtct acaggcgtca gaggagctgg caaaacttct
      121 tattgccagc ggagttatgt tataggaggt cattatgaac gatgtgcaaa atcgacgaat
      181 actggcattg ctcaatatgc tgtactatgc cttaggaaac cgaacaacct gcgatatatg
      241 tcacaaatat tttattctgg ttttgtcagt aatgaataat ctgacaatta attatggctc
      301 gcaaacgtca ccagaactga cgggtattag ttatgaagat gctaatgatt tgatcactga
      361 attaatactg tttattgaag acgtcaatgc ggataatgat atggaatatc ccgcacactt
      421 gaataaaatt caagctcttc ttaattttgc cgtggctaat taagccattc agtatctaaa
      481 attagcacct caagaggcga acttttgagg tgctttcact tagtaatcaa tccctatctg
      541 cgcttttaca ccggcatcaa acgcatgttt gatggggcgc agctcactta ccgtatccgc
      601 cagttcaaga atatcccgat gacagcctcg acccgtgata atcaccgtct gttgatgtgg
      661 acgttcattt aacgcctgaa tcacgtcttc cagtggcaaa tagtcatacg ctaccatata
      721 tgtcagttca tcgagcaaaa ccatatccag tgaggaatca gccagcatcc gctttgcatg
      781 ttgccagact tcgcggcagg cggcggtatc ggactctcgg ttttgtgtat cccaggtaaa
      841 gcccgttgcc attacctgaa actcaacgcc gtgtggctcc agcagatttc gttcaccatt
      901 aggccaggtg cctttaataa actgcacgac gcccactttt tttccgtgac caactgcgcg
      961 tgttgccgta ccaaatgccg cggtggtttt gccttttcca ttgccggtaa agacgatgat
     1021 aataccgcgt tcatcctggg cctgggccac acgagcatct actttttctt tcactcgctg
     1081 ctgacgctgt tggtagcgtt catcactcat tgggaaattc ctggtttacg gcccggttgg
     1141 gcgtcaaagg tcatgccggt tttacggcgg ctgtcatcgc ccatcagcca gaggtagagc
     1201 ggcatgatat cagcgggggt tttaagtttc tgtggatctt cggtcgggaa ggcactggcc
     1261 cgcattgcgg tgcgcgtacc gcctgggtta atgcagttga cacgcaggcg ctgctgatat
     1321 tcatcggcca gtacctgcat catcccttcg gtggcaaatt tcgacgctgc atatgcgccc
     1381 cagtttgcac gcccctgacg tccaacgctt gatgaggtaa agaccagtga accggcgtcg
     1441 gatttgagta ataaaggaag cagtgcctgg gtgagcataa aggtggcatt aacgttgacc
     1501 tgcatgacgt cctgccagac ttgcggatct tgttcgctca ttgggcaaac atcgccgagc
     1561 aatccggcat tatgcaaaac accatccaga cgcggataat taacggcaat gcgctgtgcc
     1621 agttgttggc aatcttcgga cgtgcaggtt agcaaatcga gaataaacca ctgtggctga
     1681 cgtccagttt cttcgtttat gtggctggct acctgacgta atttttcttc attacggccc
     1741 aaaagaatca ctgtcgcacc atagcgtgca tacgtcatcg ctgcttcacg accaattcca
     1801 tcgctggctc ccgtcaccag aataatgcga tcattgagta aatcttgttt tggctggtaa
     1861 tgcatggcta ctcctcaacg acgttgtctg tcgcgctgcc agtgtacttt atgactttgg
     1921 ggctttatgc ctgaaacaga agggcatttc aatcagcctg gggcgataac accgcaaaat
     1981 ggatactttg tcatactttc gctgcaataa catctctgcg agacggctaa acatgcctgt
     2041 tgtaaactgt gagccaaagc gttgtttaac caaggtgggg actcgtggaa ttgttgtctg
     2101 aatatggttt gtttttggcg aaaatcgtta ccgttgtgct agcgattgcg gcgattgccg
     2161 ccattattgt caatgttgct caacgtaata aacgccagcg tggcgagtta cgagtcaaca
     2221 atctcagcga acagtataag gagatgaaag aagaactggc cgcggcgctg atggacactc
     2281 atcagcaaaa acagtggcac aaagcgcaga agaaaaagca caagcaagaa gcgaaagccg
     2341 caaaagcgaa agctaaactg ggcgaggtgg taactgacag taaaccccgc gtctgggtgc
     2401 tggattttaa aggcagcatg gacgcccatg aagtgaactc tctacgtgaa gagataacgg
     2461 cggtactcgc agcattcaaa ccgcaggatc aggttgtgct ccgtctggaa agcccaggtg
     2521 gcatggtgca tggttacggg ttggcggctt cgcagttgca gcgtctgcgt gataaaaaca
     2581 ttcctttaac tgttacggta gacaaagtcg ctgccagcgg cggttacatg atggcctgtg
     2641 tggcggacaa aattgtttcc gcaccgtttg ctattgtggg ttccattggg gtggtggcgc
     2701 aaatgcccaa ctttaaccgc ttcctgaaaa gcaaagatat tgatatcgaa ctgcacaccg
     2761 ccgggcagta taagcgcacg ctgacgttgc tgggtgaaaa taccgaagaa gggcgggaga
     2821 aattccgcga agagttgaac gaaacgcatc agttatttaa agattttgtg aagcgtatgc
     2881 gtccgtctct ggatattgaa caggtggcaa cgggtgaaca ctggtacgga caacaggcag
     2941 tagagaaagg cctggttgat gaaatcaaca ccagtgatga agttattctt agcctgatgg
     3001 aaggccggga agtggtcaat gtacgctata tgcagcgtaa acgactcatt gaccgattca
     3061 ccggcagcgc ggcagagagc gccgatcgat tgttgttacg ctggtggcag cgtgggcaaa
     3121 agccgttgat gtaaaagaca aacgcgagga taagacctcg cgttttgctt taatcaacca
     3181 ggtgatattt ttctgaaagc acatgggcca ggtgtttgaa catattaaac accgcggtgc
     3241 ttttggcagt tggtaatcct tgttcatcta aaaagtagtc gccggtaaat accagcacgc
     3301 cattacgctg cgtgacgccg gtggcttcaa tccctgcgag cgtatcctca tgctcacgaa
     3361 tgattttgtt ggcctctttc aacagcgttt cgcggtcgat gggttgagtc tctttgttca
     3421 ttatttactc cttaaacaag gacattagtc tacgccaggc atggcttgca gacaaatata
     3481 ccacgctggt ggcaagagcg ccttactggc aactttggat tttgcatgct aataaagttg
     3541 cgtatcggat tttatcaggt acagtgtgac gctttcgtca atctggcaat agatttgctt
     3601 gacattcgac caaaattccg tcgtgctata gcgcctgtag gccaagacct gttaactcag
     3661 tcacctgaat tttcgtgagc agagtcacga caaggggttg atatccgcag agagcgagtc
     3721 catatcggta actcgttgcc agtggaaggt ttatcaacgt gcgacgcatt cctggaagaa
     3781 tcaaattagg taaggtgaat atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag
     3841 ccaaaacgat caacaagtat ctgggtagtg actacgtggt gaaatccagc gtcggtcaca
     3901 tccgcgattt gccgaccagt ggctcaactg ccaaaaagag tgccgactct acctccacca
     3961 agacggctaa aaagcctaaa aaggatgaac gtggcgctct cgtcaaccgt atgggggttg
     4021 acccgtggca caattgggag gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct
     4081 ctgaactgaa acaactggct gaaaaagccg accacatcta tctcgcaacc gaccttgacc
     4141 gcgaagggga agccattgca tggcacctgc gggaagtgat tggtggtgat gatgcgcgct
     4201 atagccgagt ggtgtttaac gaaattacta aaaacgcgat ccgccaggca tttaacaaac
     4261 cgggtgagct gaatattgat cgtgttaatg cccagcaggc gcgtcgcttt atggaccgcg
     4321 tggtggggta tatggtttcg ccgcttctat ggaaaaagat cgctcgtggt ctgtctgccg
     4381 gtcgtgtgca gtcggtggcg gtccgcctgg tggtcgagcg tgagcgtgaa attaaagcgt
     4441 tcgtaccgga agagttctgg gaagtcgatg ccagcacgac cacgccatct ggtgaagcgt
     4501 tggcgttgca ggtgactcat cagaacgaca aaccgttccg tccggtcaac aaagaacaaa
     4561 ctcaggctgc ggtaagtctg ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca
     4621 aaccgacaac cagtaaacct ggcgctcctt ttattacctc tacgctgcaa caagctgcca
     4681 gcacccgtct tggttttggc gtgaaaaaaa ccatgatgat ggcgcagcgt ttgtatgaag
     4741 caggctatat cacttacatg cgtaccgact ccaccaacct gagtcaggac gcggtaaata
     4801 tggttcgcgg ttatatcagc gataattttg gtaagaaata tctgccggaa agtccgaatc
     4861 agtacgccag caaagaaaac tcacaggaag cgcacgaagc gattcgtcct tctgacgtca
     4921 atgtgatggc ggaatcgttg aaggatatgg aagcagatgc gcagaaactg taccagttaa
     4981 tctggcgtca gttcgtagcc tgccagatga ccccagcgaa atatgactcc acgacgctga
     5041 ccgttggtgc gggcgatttc cgcctgaaag cacgcggtcg tattttgcgc tttgatggct
     5101 ggacgaaagt gatgcctgca ctgcgtaaag gcgatgaaga tcgtatctta ccggcagtcg
     5161 ataaaggcga tgctctgacg ctcgttgaac tgacaccagc ccagcacttt accaagcctc
     5221 cagcccgttt cagtgaagca tcgctggtca aagagctgga aaaacgcggt atcggtcgtc
     5281 cttctaccta tgcgtcgatc atttcgacca ttcaggatcg tggctatgtg cgagtagaaa
     5341 atcgtcgttt ctatgcggaa aaaatgggcg aaatcgtcac cgatcgcctg gaagagaatt
     5401 tccgcgagtt aatgaactac gacttcaccg cgcagatgga aaacagcctc gaccaggtgg
     5461 caaatcacga agcagagtgg aaagctgtac tggatcactt cttctcggat ttcacccagc
     5521 agttagataa agctgaaaaa gatccggaag agggtggtat gcgcccgaac cagatggttc
     5581 tgaccagcat tgactgcccg acttgtggtc gcaaaatggg gattcgcaca gcgagcaccg
     5641 gggtattcct tggctgttct ggctatgcgc tgccgccgaa agagcgttgc aaaaccacca
     5701 ttaacctggt gccggaaaac gaagtgctga acgtgctgga aggtgaagac gctgaaacca
     5761 atgcgttgcg cgcaaaacgt cgttgcccga aatgtggcac ggcgatggac agctatctca
     5821 tcgatccgaa acgtaagttg catgtctgtg gtaataaccc aacctgcgac ggttacgaga
     5881 tcgaagaggg tgaattccgc attaaaggtt atgacggccc gatcgttgag tgtgaaaaat
     5941 gtggctctga aatgcacctg aaaatggggc gtttcggtaa atacatggcc tgcaccaacg
     6001 aagagtgtaa aaacacacgt aagattttac gtaacggcga agtggcacca ccgaaagaag
     6061 atccggtgcc attaccggag ctgccgtgcg aaaaatcaga tgcctatttc gtgttgcgtg
     6121 acggtgctgc cggtgtgttc ctggcggcca ataccttccc gaaatcgcgt gaaacgcgtg
     6181 cgcctctggt ggaggagctt tatcgcttcc gcgaccgtct gccggaaaaa ctgcgttatc
     6241 tggccgatgc gccacagcag gatccggaag gtaataagac catggttcgc tttagccgta
     6301 aaaccaaaca gcaatatgtc tcttcggaaa aagacggaaa ggcgactggt tggtcagcat
     6361 tttatgttga tggcaaatgg gttgaaggga aaaaataacc tttaattccg tctggttttt
     6421 ataaacaaag ggtcgcgaaa gcggcccttt ttt
//
LOCUS       DAPQZD010000228         6405 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPQZD010000228 DAPQZD010000000
VERSION     DAPQZD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.230"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..122)
                     /locus_tag="RO454_004589"
     CDS             complement(<1..122)
                     /locus_tag="RO454_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0936312.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAG"
     gene            335..1126
                     /locus_tag="RO454_004590"
     CDS             335..1126
                     /locus_tag="RO454_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDY0936313.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1136..1978
                     /locus_tag="RO454_004591"
     CDS             1136..1978
                     /locus_tag="RO454_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDY0936314.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            1988..2764
                     /locus_tag="RO454_004592"
     CDS             1988..2764
                     /locus_tag="RO454_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDY0936315.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            2774..4315
                     /locus_tag="RO454_004593"
     CDS             2774..4315
                     /locus_tag="RO454_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDY0936316.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            4312..6384
                     /locus_tag="RO454_004594"
     CDS             4312..6384
                     /locus_tag="RO454_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDY0936317.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
BASE COUNT         1558 a         1591 c         1717 g         1539 t
ORIGIN      
        1 cccgcggcct tgacgatatt atcgactctg gccccttcgt aaccatattc ggcaaattca
       61 ttaaccgcgc ataacagcag cttttctttc aggctctgta cgttgctggt atcaagatac
      121 ataacgcttc cccacgacct cttcgtggtg actatcataa cctgatttga tttcagcttc
      181 agaaaaaagg gcaaaaactt gtactttcat caggttatca aatgtcctgt gatcgccatc
      241 gcatatcaac caactagatg gttgatcttt ttatcctcca gtcgcaagct aatcatcact
      301 aaggaaacaa aattcaaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt
      361 gattaccgga gccgctcgtg gtattggaaa gggctgtgcg ctggagcttg cgcgtggcgg
      421 atttaatctg ctgattaatg atcgtccgga tgcggatagc gtagaaaaat tacatgccac
      481 acaacaagaa tgtctggcgg aaggcgtgga agtgatttgc ttccctgctg atgtgggcga
      541 tctctccctg catgaagaga tgctcgacgc agcgcaaaac cagtggggac gtctggactg
      601 cctgctcaac aacgcaggca tttcagtgaa aaaacgcggt gaccttctcg acctcgaacc
      661 agacagcttc gaccaaaata ttgccattaa cacccgcgca ccgttcttcc ttgctcaggc
      721 attcagtaag cgcttactgg cacagccgaa accggaagca gaactgccgc accgctcgat
      781 tatttttgtc agttccatca acgccatcat gctggcgatg aatcgcggcg aatataccat
      841 tgccaaaact gccgtctctg ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca
      901 aattggcgta tatgaagtgc gtcctggtct catcaaaacc gatatgacta ttccggcaac
      961 ggcctattac gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc
     1021 ggcggatatt gcttccactg tccgcgcgat ggcggaaggt aaacttattt acacctgtgg
     1081 tcaggcggtc gccatcgacg gcggcttaag tatgccgcgc ttctgaggct gaatcatgag
     1141 agatctgtta caacgcccgg atttattcag tatcaatacc gcgacactgg gctataaaac
     1201 gccgctaccc gccattattg atgcctgcgc cgcacgcggt attggcgcga ttgccccctg
     1261 gcgcagggag ttgcagagtg aagatctcca gcaaaccgcc cgccagcttg ctgccagtaa
     1321 catgaacgtt tccggtttgt gccgtagcac ctactacacc gcaccgacgc tggcggagcg
     1381 caaactggcg attgacgata accgccgggc gctggacgac gccgctgtac tgaacgccgc
     1441 ctgttatatg caggtggttg gcggtctgcc aatgggaaca aaagatctgt acgaagcacg
     1501 ggaacaggtg aaacaaggga ttcgccagtt gctgccacat tcgaaagagg tcggcgtgcc
     1561 aattgcactg gagccactgc acccgatgac tgctgccaat cgttcttgtc tgtgcacgtt
     1621 acgtcaggcg ctggactggt gtgatgaact cgatcccgat ggcgaatttg gcctcggcgt
     1681 ggcggtagat gtctaccatg tctggtggga cccggatctc gccagtcaga tcctgcgtgc
     1741 cggaaaacgc atcctcgcgt ttcatgtttc cgactggtta gtgccgacca ccgatctggt
     1801 caatgaccgt ggaatgccgg gagatggtgt catcaatatt ccgtcaattc gtcggctggt
     1861 tgaaaacgcc ggatttaatg gcgctattga actggaaata ttctcaccgt actggtggca
     1921 gaaagatatt aacagcacgc tggatattag cgtcgatcgc atcgcgcatt attgttaagg
     1981 aatgatcatg aatgaccaac ccgttctgtt cagcagagcg gcggcttctt gccgcctgac
     2041 attgaaccgt gaagataaat gtcatgctat taatgaagaa atgatcgaat ctctcgacca
     2101 ttatttaaat gaaattgaaa atgacacggc attacgctta gtcgaattaa cggcaaccgg
     2161 cgataaattc ttttgtgcgg gtggcgatat taaatcgtgg tcggcctatt cgccattaga
     2221 tatgggcaga aaatggatta aacgcggtaa tgacgttttt aatcgcctgc gaaacttacc
     2281 ccaattaacc gttgcgaatc tcaacggtca taccatcggc ggtggtattg aacttgcctt
     2341 gtgctgcgat attcgtatcg ctcgccccgg cgcgaaattc tctaacccgg aagtgatgct
     2401 cggcatggtg ccaggctgga tgggcattga gcgagtgctt aatcaggttg gtcctgtggt
     2461 cggtcgccag atgctgatgc tgggtaaacg cctgacggcg caggaagctc agacagcgaa
     2521 tttaattgat gaagtggtag aaaaagaaca ggtggaaagc tggatggcga accagttagc
     2581 gcagctggaa aaatgtggtc cggtggcact ggcacatatt aagcaactga ttctggcgct
     2641 ggaaaataaa cacgccgatt actcgcacca gttgctggct ggattaatgt ccgccactca
     2701 ggattgccaa caggcgacgc acgcatttgc cgaaaaagcc ggcgtgagct tccacaatca
     2761 ataaggaaat tgtatgcgta agttatcaac cgccgagaca ctggcggcgc aaattcagga
     2821 cggcgcaacc attgccatta gcggcaacgg cggcggcatg gtggaagctg accatattct
     2881 ggccgccatt gaagcgcgct ttctgcaaac cggacacccg cgcgatctga cattaatcca
     2941 ctcgctgggt attggcgatc gcgacagcaa aggcactaac cgttttgctc acgccgaaat
     3001 gctcaaacgc attattgccg gacattttac ctggtcgccc aagatgcagg aactggtgaa
     3061 aaacaatgct attgaagcct actgtttccc tggtggcgta attcaggcac tgctgcggga
     3121 gatcggtgcc ggacgtccgg ggctttttac ccacgttggg ctgggatcgt ttgttgatcc
     3181 acgcaatggt ggcggtaagt cgaatgaatg cactaccgac gacctggtag aactgattga
     3241 aatcgacggt gagaccaaac ttcgttatcg ccctttcaaa gtggattacg cgattttgcg
     3301 tggcacttat gccgatccac gaggcaacgt tagcctcgaa gaagaagcga ttgatatgga
     3361 tagctattcg atggcgctgg cagcacacaa cagcggcggc aaagtgttcg tacaggtacg
     3421 cgatgtgctg gaagcaggta ccattgaacc acgtcgggtc aagctaccag gaattctggt
     3481 tgatggcatc gttgagcacc gcgaacaacc gcaaacctat cttggtggtt acgacctgac
     3541 catcagcggt caacatcgcc gtctaagttc taacgacgct attgaactgg ttagtcatcc
     3601 ggtgcgtcgc ctgattgccc gtcgggcagc gcgggaactg gtggcaggcg cttcaaccaa
     3661 ctttggcttt ggtattcccg gcggtattcc tggggttgcg ctgcgcgaag gcgtgcctta
     3721 tcaaagtttg tggctgagtg tagaacaggg tgtgcataac ggcatgatgc ttgatgatgc
     3781 tctgttcggc tgcgcccgta acgccgatgc cattattcca tcactcgatc aatttgaatt
     3841 ctacagtggc ggcggcatcg atatcacctt cctcggcatg ggagagatgg atcaatacgg
     3901 taacgtcaac gtatcccatc tcaatggcaa tctgattggc cccggcggat ttctcgaaat
     3961 tgcacaaaac gcccgtaaag tggtgttttg cggtacgttc gacgccaaag gcagcaagat
     4021 tgatgtaacg ccagatggct tgcatatcgc ccagtcaggt caaatcccta aactggttac
     4081 ccaggtggaa aaaattactt ttagcgccgc ctacgcacag caaagtggtc aggaagtgtt
     4141 gtatatcact gaacgcgcag tattccagtt aacggcagaa ggcgttgaat taattgaaat
     4201 cgcgccgggt gtggagattg agcgcgacat tctgccgtat atggccttcc gtccgattat
     4261 caggcaccca cgtctgatgg aaagtagcct gtttatgcca atggaggatg catgagtcag
     4321 cttgatgaca ccatccttga tgcactgacg cacgttactt tcccaaaggg ttttgcacag
     4381 gcagaacccg catgggttgt cacggttgac ggcgttgatt acccactctg gaaaacagat
     4441 gccctggtag tcggcagtgg tgcagccggg ctacgtgcag ctgtggaact taaacgccgc
     4501 cagcaaaatg tgttaatcgc caccgccggg ttatatatgg ggacgtcggc atgttctggc
     4561 tctgataaac aaacactgtt taccgccgct actgcgggca acggcgacaa cttcaccaaa
     4621 ctggcagagg cactggcgag cggtggggcg atggatcacg acaccgctta tgttgaagcg
     4681 gtaggttctc tgcacactct tggcgggttg caatatcttg gtctggaatt accggaagat
     4741 cgctatggcg cgattcttcg ttatcaaacc gaccatgacg aagccgggcg tgcaacctcg
     4801 tgtgggccgc gtacctcaag gttaatggtg aaagtgctgc tggaagaagt acagcgtctt
     4861 gctatcccaa tgttgaccag tgcaacagtg attaaactgc tgcatcagcg tgacgaaaac
     4921 ggtgaagacc gtgtggcggg ggcaatcctc gcgaccggtc atcgcgccca taacccatgg
     4981 gggctggcaa ttgtgactgc gcccaatgtg gtactggcaa caggagggcc tggcgagctt
     5041 tatcgcgaca gtgtttatcc acacaaatgt ttcggctcgc tgggtctggc gctggaggaa
     5101 ggtctgacgc taaccaatct gactgaaagc cagtttggta tcggcacacc gcgcagcacg
     5161 tttccgtgga atttgtccgg cacctatgtg caggtgatcc cgtatatcta ttccgtagat
     5221 gctgagggta acgagtataa cttcctcgcg gattactatc gcaccaccca ggagctagct
     5281 tcaaacatct tccgtaaagg ctatcagtgg ccgttccacg ccacgcgggt aatggatttt
     5341 ggctccagct tgttagatat ggcagtagcg caagagcagc aatcagggcg tcaggtattt
     5401 atggatttca atcgcaatcc tgaagcggtg ccgggtgacc tgcccttctc attagatcga
     5461 ctggacgacg acgttcgcac gtatctggaa aataacgacg ctctggcacc atcgcccatc
     5521 gaacgcctgc aacgaatgaa tccgctgtct atctcgctgt ataagatgca cggttacgat
     5581 ctcaccacgc agccattgca gtttgccatg aataatcagc acatgaatgg cggcattgaa
     5641 gtcgatatct ggggacaaac atctctgccc ggttgttttg cggtggggga agttgctggc
     5701 acacacggcg tcacccgacc tggcggcgcg gcattgaatg ccgggcaggt ttttgctgtt
     5761 cgtctggcac gttttattgg ttgcacgcaa aagcggaata ttgatggaga tatagcacag
     5821 ctggcagctc cggcactggc ttctataaga gagatcatta ctcaggcgca cgataacggg
     5881 accgggatgc cgttgtcggt tgtgagagaa aaaattcagg cacgaatgtc tgaccatgcg
     5941 ggatttattt gccatgccga taaagtccga cgcgccactc gtgatgccct gctattgaac
     6001 gaatttgtcc aacgacatgg attggcaatc aaacatgtgg gtgaagttgc cgagctgttt
     6061 atgtggcggc atatgacgct gacttctgcc gcggtattaa cacaattaac gcattatatt
     6121 gatgctggtg gtggtagccg tggggcgcgt atggttattg atccacaagg caaatgccta
     6181 ccacaaactc gtcgcggcgc aaaagaagaa tggcgttttc gctctgaact cgctgaagac
     6241 aaaaatcaca aattaacaat tcaatattcg caaggttctt ttattaccga agtaaagtct
     6301 ttacgtatgc aaccgtgtat taacggtatt tactttgaaa aaaactggcc agacttttta
     6361 aagggagaca tttacacaca ataatttcca atatttcctc tgtac
//
LOCUS       DAPQZD010000229         6390 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPQZD010000229 DAPQZD010000000
VERSION     DAPQZD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.231"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(370..1563)
                     /locus_tag="RO454_004595"
     CDS             complement(370..1563)
                     /locus_tag="RO454_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDY0936318.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
     gene            complement(1761..4394)
                     /gene="mngB"
                     /locus_tag="RO454_004596"
     CDS             complement(1761..4394)
                     /gene="mngB"
                     /locus_tag="RO454_004596"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDY0936319.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQLGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIGTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRTFSYRIV"
     gene            complement(4412..6328)
                     /gene="mngA"
                     /locus_tag="RO454_004597"
     CDS             complement(4412..6328)
                     /gene="mngA"
                     /locus_tag="RO454_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDY0936320.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
BASE COUNT         1676 a         1673 c         1365 g         1676 t
ORIGIN      
        1 gtaattaagc agcataagaa tatgtcttat tatcattaaa atacattcta tttccatcat
       61 ccttaagata ataaaacagt gagtgatgga taatttcacc ccaaaaaaaa gcaagcatat
      121 aaaattaaaa aacatgactc cactttcatt tttatgaaat ggcatcgtga agaggcgcca
      181 gccaattata aagatcttgc tcctcaaaaa atgaaacaac tcagcctgag ataatatttt
      241 tgaccgaagt taacattaaa aatggttaat agtttgcgcc tctcacacag gtccaggcaa
      301 ctgcgtaaca ggcaaatcaa attttcgtaa acagtgggca tcatcagatg cccacaaaat
      361 acagctgatt tacattttcg tctttacacg tgcaatagct tcttcataag aaggcttatt
      421 tttttccgta tacgccagtt gataatgctg caatgcttca gcattctggc ccgaggtttc
      481 ataaattttg gcaatgttat aataggagtt ggcgcgcgtt gtcgccgcat ttaccccact
      541 cgctaacgag atcgctttac gatttgccca aattgcttcc gctatgttgc catttttctg
      601 atacgccaga cccaggttac taaatgcctg tccgtaattg ccgtctaatt ctgttgcctg
      661 ttggaagagt tgaatggctt gctcaagttg accggtttta taaacagctt caccttcgcg
      721 attatatttt cttgccagtt gagtatctcc actgttttgc atcgcaggcg aagccggagc
      781 tgaagagtcc gtcgcaggca ttatcacagt ggctaaatcc ctgacgtcta atgtggtgcc
      841 gttaaaattg gcgctggtaa cagagttgat atcttcaaac tcgccgtttg gattgagttt
      901 aaacacggtc cagatgttcc ctcgttttcc tgcagggaca ctataactac gtaccaggga
      961 gctccctaca taaacaaaca cttttgcctt actggcagag agataattac tgttgggcag
     1021 ccctttattg ctatagtcct gcacggcata gatgtaactt tcaccaaaat gctttttgct
     1081 gatagtgaca gtttctggtc catagctatc cgtatcatca acatccagat tggcatcagt
     1141 tccctcttta ctatcaaaat agatatgtcc gccagggaag atcaaatgcg aatcaagatc
     1201 aaaaggcttt tcgccccagc tcaaaactac acgcatgcca tccagattcg tcatcaccgg
     1261 actgatagca taagtcatgc cagcgcacgg acatttgacc accagattgg aatacccctc
     1321 ttttttgacg attaatagag catctttatt atcagcaaat gaagtcccca aactaatgct
     1381 gcccgaagta tttgtgatac cagaaacaga ttgtgcacca ttgcgttgca gcgtcaccga
     1441 tgccccttca atgcgtttat ctttcactgt cgcacttaaa atatcaatgc ttacatcggc
     1501 ggcataagca cctgcattca tgcccaacag cgcgagagca actggcaaaa caattttctt
     1561 catgtataaa tccttttttt aatgaatgta aggtggaaat ttcccagtta ttattatcgt
     1621 gttaaaaggt gaaaaaaaag aaataagtac acttttcaga ttaacaccca ctcaccttga
     1681 aacaaaaagg ccatccggag atgacctgaa ctttctgaga aacgctgttt tttcatgtgg
     1741 tttgctttat tacacaccat tcagactatt cggtagctaa atgtccgcga ctgtcccggt
     1801 aaaaagagag ctaattcttt taaaacctca tttttttctg tattaaagcg ttcattcatc
     1861 atggtttctg tacacgtcat caccttcctg ctaaacgcga cggtcgcatc acagcttgtc
     1921 aattcagccg gattaaacag ccgcaatatt aattcctgtc tgtcttctgc tttttttagc
     1981 gcgctgatca gacatcccac tggtggcatt ttcagcaaac tatagctttc cggcacattg
     2041 aatttggctt tattaagctt catcgcatcc cacgggattt tgttataaca ctgaactggc
     2101 gtcagccatg cgcgtgcctg ctgggctaca ccggcagtta cgggagtacc gatataacta
     2161 aataaactca gacgacagga aaatgatccg cgtaattgtg aatcggacac aggcatttta
     2221 attcctgaag gacgccctgg ccttaaaagt aaatcttctt ttcccagtaa tcccacacca
     2281 cgcaacaacg taatagcaaa ggttttattc tcttctccaa tgacttcaaa ttcacgtaat
     2341 ccttcgctaa aaacggctat gccgttacgc ccttcctgta agacagcata gttgagcata
     2401 ttccataccg gaaccggcgc ttctttccag ccttcttgct gccagacatt cattgcgcaa
     2461 tccttcactg gccgcgtcaa tgaaccaaac tgcgtatccg caagaacaac atctgtgtta
     2521 aaaggcgtgg gaatcaatac acgaatacgg tgatcatcgg cctggttatc aagattaata
     2581 tccgcatcaa tgcgtctgct gttatggcta agagtgatca ccgtctccac ccctaccctg
     2641 ccagaacatt gctttgcgcg acgctctgac agattacgcg gcaccgctat ttcatagcga
     2701 attatggccc tactctgcca ggcttcatga ataatatcgt attgcggttt cgcattcgct
     2761 gaggtaatgg cccactcttc ttttgctggc gaataatcat attcatcccc atcatcggaa
     2821 ccttcttcaa tttgaaacac ccggtcatag cgcacgccac tatctttatc aaccagacga
     2881 agtgaaccat cctcattgag tgcaatttgc cagaaagcat tttccagtat tccctctgtt
     2941 ttactttttg gcgtgaccac attgccaagc tgattcgctt ctatatagag cgtgcgatac
     3001 cccatagaag ggacaatttg gctgatctga atatcaaact ccataaaggg atcataattg
     3061 ccgtaatgaa ctatttgccg atcaataagg ccgggatcga tctcacgggc atgtcgaata
     3121 aaatatggca caggctgacc gcgaccgtct cgcaaattaa actggctacc gcgcagtcga
     3181 acagttgtgt tgataacttc ttcacgcggc cagggcatca ggttaaacag gacgagtttg
     3241 tcggcgtcgc tctgcggcat gttgtctgct attttgcgca tatagaaccg aattagatta
     3301 tccgccatgt cttctgccag ttcgaagcgg gcgacaatct cgcgatgaac tttatcactg
     3361 cagcagcagc cgatgctgtc atgggcgtgg ttttttaaaa tttctttcca catcttctcc
     3421 agcaagccgt ggtgataatc aaaacctaaa gtccaggcca gcgttgccaa cggttccagc
     3481 agattaacaa tcttattttc aatgcgtgcg tgggcaattt tgatatccat gcgcgtggaa
     3541 ccgatggtgc gatgcacgcg catatatttg ccatcgatga attcaccttt caggatggcc
     3601 agactttcac gctgcgcttc aatctgctcg aatacctctt caaagcggct catcacaaac
     3661 tttcgttgag gataaatttc gcgcagtttc tccatcactt cgaagatatt ttgctgcaag
     3721 ggcatctggt catgaccatt cggcaataaa atttctttgg ttactgacgc tttttccagc
     3781 acgtcaaaat aactgtcgag gcgtttacgt aatccatttt cgtccgcagg taagtactta
     3841 ccgatggcgt agcccagcgg cagcacctgc gccgtcactt cgctaccgtc actgctttgc
     3901 cacaaaaact cggttttatc agtaccgtgg cgctcagaac atccgcgcca gaacatggtg
     3961 cgggtaatgc caaatccatt gtatatatgt ggcagttgcc cggacatgcc aaaggaatcc
     4021 ggtaaataac ctattttcat cggctcgcca aacgcgaggc agtcacgcat tccgtacatc
     4081 agattacgga ctatggattc agcagaaaca atcgtggtat cggtctgggt ataccaggga
     4141 ccgataatta atttcccgcc ctcgaccaac ttcttcacac gatctttgtt ctccggtttg
     4201 acagcgaaat aatcttcgag gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg
     4261 ttgtcctgtt ccagtctgca caggatctct tccatattat tgaccagcag aatgcgagac
     4321 tcttctgtgg tgaaatacca ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga
     4381 gataccgctt tcatcgtcgt ttcctttttg tttatggcat tacgccatca gtcagatagt
     4441 ttccatgctt taccgcatga tgccgccaga tcaggagaat tgcagtcgag attgcagccc
     4501 ccaccaacgc cgcaccaaac caaccaattg ccgccataac accacccgcg ccattatcat
     4561 gaagtaaaaa gagcgagaaa atgccggcac cgggtgtcga aaggccgata ttcatcgcac
     4621 cgacgatagc gcccgttacc atagatccca gcacaaacga accaataacc cgcagcggat
     4681 cttcaatcgc catcgggatc gccccttcgg tgatacctgc caatcctaac agccaggtgg
     4741 atttcccggt ttcaatttca aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg
     4801 ttacggtaaa tgccgaaacc attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc
     4861 cgttcgccat tgcccccaga cagaatgcat aagcggcttt gttcactggc ccaccaaggt
     4921 caaaggaaca cataaaaccg agaatggcgc ccaacaacag tgcgttattt cctgacagac
     4981 cgttcagcca ggcggtaagc gagttattga tccatgcgac aggttcccct actacaaaca
     5041 gcatcagact acccgccccc aacgtaccga gcaccgggta gagatagaaa gtcaggaatc
     5101 cgttgaattt actgctaaga cgtagatgat ttttcaccca gcgcatcaga taaccggcta
     5161 tcaatccgcc aacgaccgcg ccgagaaacc cggaaccaat catgttggcg gcaagccctg
     5221 ccgcaaagcc tggcgctaac gccggtttat cagccagtga ataggcggta taggctgcga
     5281 gcaccggtac catcaaaatc ccgagcatcc cgccgcccag cttgcggtac atccacaacc
     5341 aggagttttc ttcattaaac aaatcttgta gcccgaagat ttgcgacagt aataccgcga
     5401 ccgccagcac cgtgccaccc gcgacaatca gcggcacggc gaaagagatc ccgctcaaca
     5461 gtgcctgctt cagctccgtt ttgacacttt tcaccggttg cgtatcttgc tgtacggtac
     5521 gcgtctcatc gctacgcttg agggtaagcg cttgttgaat caacgcttct gcatggcgaa
     5581 tcggctcggc aacaggcaca gaaagcgcgg gaatgccgtt aaaacgctcg ctctccttga
     5641 tagcgacttc cgccgcaaaa atacaggcgg ttgcactatt gagttgctcc gccgttaaac
     5701 gctcttcaat gccgttagca ccttgctttt caacgtagat attcacgccg agtttgcgtc
     5761 cggctttttc cagatattcc gcagccatgt aggtgtgagc aatacccgcc gggcaggccg
     5821 ttacgcagac gatagttggc gcataagaaa aagaggcagt atgttgcgcg tcccccttgt
     5881 catccagcgc cgagagcaat tcatcgggcg tcgttgccga ctgaatacgc gccctgagct
     5941 catcatccgc caggcgcgtg gtcagcgcgg taagcagttt catatgcgta gtacctgctt
     6001 cattgggagg aatggccagc aataccacta aatcaaccgc ttccgggcca tccacgcctt
     6061 cccactgaag cggctcgctg agtgtggcga ccgcaaacgc cgcttctttt accgcagcag
     6121 ttttgccatg cggcacggcc agcccttcac ctaaggcggt cgggccgagg ctttcacggc
     6181 aatacacttc ttccagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt
     6241 gagtcaacgc gtggatggcc tcttcacggc tggtaaagcg cacattcaga cacagcgcat
     6301 cgcggtgggt cagagtcgtc aggttcatca tggttctcac ttgatcattt ttatagtcgc
     6361 gcaagtgtgc ctgataaaac aatacattta
//
LOCUS       DAPQZD010000230         6388 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPQZD010000230 DAPQZD010000000
VERSION     DAPQZD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.232"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..846
                     /locus_tag="RO454_004598"
     CDS             1..846
                     /locus_tag="RO454_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDY0936321.1"
                     /translation="MEHLLKSESTALRESICAVIAGKDCYQADTLSLLSGVKIISRFG
                     TGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGE
                     SHGCELQGKRIGLVGYGNIGKILAKRLSGFDVELLAFDKQPDYQVADKAGVQFVSIED
                     IFMQSHAIIVLLPFSSELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERN
                     VFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYFAGRK
                     IKNVL"
     gene            complement(1146..2522)
                     /locus_tag="RO454_004599"
     CDS             complement(1146..2522)
                     /locus_tag="RO454_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDY0936322.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNVQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(2522..2950)
                     /gene="tssE"
                     /locus_tag="RO454_004600"
     CDS             complement(2522..2950)
                     /gene="tssE"
                     /locus_tag="RO454_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDY0936323.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(2954..3490)
                     /gene="tssJ"
                     /locus_tag="RO454_004601"
     CDS             complement(2954..3490)
                     /gene="tssJ"
                     /locus_tag="RO454_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDY0936324.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(3471..4550)
                     /gene="tssG"
                     /locus_tag="RO454_004602"
     CDS             complement(3471..4550)
                     /gene="tssG"
                     /locus_tag="RO454_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDY0936325.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(4514..6274)
                     /gene="tssF"
                     /locus_tag="RO454_004603"
     CDS             complement(4514..6274)
                     /gene="tssF"
                     /locus_tag="RO454_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDY0936326.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1468 a         1711 c         1710 g         1499 t
ORIGIN      
        1 gtggagcatc tgcttaaatc agaatccact gcattacgag aatctatttg cgcggtcatc
       61 gccggaaaag attgttatca ggcggacacg ctatcattgc tgtctggtgt gaaaataata
      121 tcgagattcg ggacggggat agacaatatt gatcttcgtg cggcacagca atccggtatt
      181 gttgtcaata atgcggttgg gattaattca aatgcggttg cggaatttat cattggactt
      241 atcttcgcga gcatgagaaa tatccctggc agctatcatg cgatgcaaaa tggctactgg
      301 ggtgagtcgc atggctgtga attacaaggg aaacggattg gcctggttgg ctacggtaat
      361 ataggtaaaa ttctggcgaa aaggttatcc ggttttgatg ttgagctttt agcttttgac
      421 aaacaacccg actatcaggt tgctgacaaa gctggagttc agtttgtatc aattgaagat
      481 atctttatgc agtcgcatgc catcattgtt cttttgcctt tttcttctga gctggagaac
      541 ttcattagtc ataaatattt gtcgatgatg cgcaatggtg ctctgatcat caatgccgca
      601 aggggaaagt tgctggatga gggcgcttta cttcaggtga ttgaagaacg aaatgtgttt
      661 gcggcgctgg atgtgtttag ctctgaaccg ttggcgcaat ttagcccact gctacatgcc
      721 aaaaatatca ttacaacgcc gcatattgcg gccgccaccg ttgagtcata ccagcaaacc
      781 gggatacacg ttgcccagtc gataattgat tacttcgcag gaaggaagat aaaaaacgtg
      841 ttgtgagttc agcgaacaaa gattttaaat acaaaaaaac cgcaatttgt agaacacatt
      901 gcggtttgtc agcattctaa taaattggtt gcgggggcca cagtcaagag gttaatccaa
      961 tcttttgaaa cttaaggtat tcactgttcc cgaacccaag ctatctaccg aaatatacac
     1021 tcattatctg atgtgaatca acgagtagat cgaatggcct gttcctctct tttttgtaca
     1081 caaagactcc ttttaaaaaa tagcaaggtc acatatccct tattggtaca taaatccccc
     1141 gatgtttact gactttgttc caccttcgta tccagtaaaa tgacatagcg attcagcaac
     1201 tgatttaacc gggtatcaat ctgcgtcaac agcgtcggcg aaatctctcc ggtctgtttt
     1261 tgctcttcca gttgccgaag cagttcttcc agcggcggct cctttaacga ctgccggatg
     1321 ccgtacaccg ccgtttttag ttccgaaccg gtcagatatc tccccttacg ttcatccagt
     1381 gcatccagtt gtgccgacag gcgctgtagc tgactctgcg cctgtgcgta gccattcagt
     1441 gcctctgcgg acagggcgct cagttcccgc tgtttacgcc actgcacgtt cagcgccctg
     1501 acctgaggat tatccggcca cagggcatca agctggcgta gctggcgata tccctgctcc
     1561 agcggccagc gggcaggtaa tttgtttaac tgctccagtt gcatctgact tgccgcaatt
     1621 gttctgtcct gagccagcag cgccttatcc tttacatcca gctttgccag ttcgccgctc
     1681 tccagcgact gcggcaacgg catgacagac agttgtattc gctggattag cgcgtccggc
     1741 tgattaagct gcgaccagcc ccataatcct gttccgccga gaccggccat cacaaccatt
     1801 ccggccataa atatcggcca tgtccgctgt cgggagattt caggggatga cacgggtgga
     1861 ctggcatcct tatcgttttg ctgagcgggt ctcgttgcaa aaaccggctg tggactttct
     1921 tctgactttt tcggttgatt catttcccgg cggggtaatt cagggggagt gatagtggtt
     1981 tccgctccct gaggcgccag ccgttccaga cgcaatgcgg tattgcgaaa ctgcccggcc
     2041 agcacatcca gtttcgtcat atgccacagt tcacactgtt gcagcgtggt ttcgatggcg
     2101 gaaagatgct gtacacaacg gtagatctgc ggcagatcct gatactgaat atccagcgtt
     2161 ctcagtgcct gctgcatttt ttctgtgacc cagctgagca gtgatattct ggaatgtacc
     2221 tgtaccggcc agaagtcagc ccagcgctgg agcagtatat ccagtgagcc gagtccgtct
     2281 gccatccctg ccagcccctg ccgtctggtt atcgccagta cgtaacagac cagcgtctgt
     2341 agttccacgc cattttgccg gaacagcgcg aggcagagtt tttccacctg acgccagtcc
     2401 acatccggtc gggccgggtg agtcagttta ctcatttcag cctgcagttc accggattcc
     2461 ttataagtac agggatcgcc gcccatgacg aactggctga taaaattcgc gttacttgcc
     2521 atcagtacgc ccgctcctgt tgtttgagat gacgcagaac aattcgtcct tccggagaaa
     2581 atacgccatc aaagcgtatc cagccggtat cgggaagctg cgcgtggatc aggtaattaa
     2641 gatgccccgg acggggctgg gggagtaact ccacctgtat ctgactcagg cgtggttcat
     2701 aacgcagcag cgtttcttca atctgacgca tcaggccatg aatacccgca gccatcccct
     2761 gatggatcag actgagatcc ggcagaccgt aatccggcaa atgcgccagc gtgcctgccc
     2821 ggcagttgat aatgcgttgc atattgtcca tcacggaaag gataagctga tcccgctctc
     2881 tgacctgttc cagcggtaac tcgccgctga aactgaacgt cagcatttca tataaggaag
     2941 gtgtcgacat cggttattta tccttgagag gcagcagtcg caggtcgctt ctcatcagtt
     3001 caatcgagcg cggcttgtcg gcctccagtt cgtccctttt gatcaccaga cgccagctgt
     3061 cgcttttttc atcagggtga taaaactgcc caataatggc gacaaactgc gtctctttat
     3121 ccagcggcac attcagggat gccgtgcctt ccggttttac ccagacggtg tgttttgcca
     3181 gtacgttctt ctccagcgtc ttctcttcct gcataaacag ggtgtcataa tccgccttgt
     3241 caaaatcttc gcgggttttc agttgccaga catgcaccat cgtggaaagc ggcgtatctt
     3301 ccgcatccgt attcagggca cttcgggaga agaaatcgag atgcagtatt ttgatttgct
     3361 tgtagaaaac ggctgaggcc acgcttttcg attcatccac cactttctga atcagcccgc
     3421 agccgctcag aacaaccgtc agcatcgcca tacagagcag cgccggacgt ttaaaacgaa
     3481 taggaaacat gagtcatact ctccctgtta cgaatggaaa aagcgggtat taatccctga
     3541 taacgcccca gactgaccgt cactgttccg gtcgcgggcg cggcaaagga ggcgcgaagg
     3601 atgccggtac ggctaatctg tacacgctgt tttcccagcc gggcagcagg caacaggctg
     3661 acgggcagcg tcagttgcag tcgggcctga cagcgccagc ccagataaac gcgcagcagc
     3721 accagcaaat cgctgtgcag ttgcccgccg ggcagccagc cacgtgcttc tgcggcatct
     3781 tcggtataca gtttcagcag caactggctg ttgacgtcgg tgccggtacg ccccagcagc
     3841 gttcgcgttt tcaggctgat gggtgaattt tttgacagac ccgcaggggc cgggagaatt
     3901 acaggctgcg gatcatgagg caccaccgtc gctttcgtca gcggggcaag cagtcccacc
     3961 agtgccgtaa cgccttccgc cgtgcgcgtc ggcaggcgca tcacgctgag cagggccaga
     4021 aaacgggata ccggcgtggc gatatggttc tcgctgccgg gaatacccag cccgatgagt
     4081 cccagcaggc aacgggaaat gtcatccact gcacctgctt caaacgacgc cgggtagtta
     4141 tatttgcgcc agatgcggta atactgggtg ataaagcgat ggttaaaaat atcgagaaac
     4201 gccatcaccg cgtcatgtcc gtcatgacgc tgggtaatgt agtccagata ggcggttggc
     4261 agtggcgaat ccacgccgta caggccgaga aaagtggtgc gcactgtggg tggcgcgtcg
     4321 ggattgtcca cgtcacgttc aacgttcttt aactcgctga ccggaaagcc catccccgga
     4381 tgaggacgaa agcgcaccgc atcggcggcg gggctgtcgg tactgcccag cggcgcgttc
     4441 tgctggcgct gttccagcaa ctggcaaaaa cggtaaaaat tgatccgcgc aatatccggg
     4501 gccagccggt cggtcagccc ggtacgcgct ggctgtgatg ctctgtccat tgcaggcact
     4561 ttcctgtggg ttgcagaatt aaggttagtt gagtgaataa gtggatatcg gcatacagtg
     4621 cgaagaagcg gtgcagcagt tcaccaaaca gcgtgatatc accttctccg gcaaagccgt
     4681 tactgtccag cgtcacctga atatccacgc cacgcagcag aaagcctctt tcaaaacgct
     4741 gtatcaggct gtgctgaacg tcgacaatcg ccgccagacg acggcggttc atctcgcttt
     4801 cggtccagtc gtacagcgcc agcgtgccgc gcagaatttc ggcgttatcc atcatcgata
     4861 aaaagttaga accaaggtgg ctgagcaccc gccagtggaa gcggtcacgc gccgggggat
     4921 agcagggctg cgtcggggcg cacaggttgc gcacccgcag gacgttctgt gaggcatgaa
     4981 ctggcgtatc gagcagcgtg ctctgtaaag ctttacgggg aagctgcccg ttagtaccgg
     5041 tcaggcttaa ggacagggtt tcatcctcca gcatccggtc agtatcaaac gcatcgccgc
     5101 ccagaatcag ccaggtgtca tgcaggccgg aagggccgcg ctttacccgc gtgtggtaat
     5161 agcgttccgg cgcatcgtgg cgcagcatcc cgccccggtg acgaaagctg gagaatggca
     5221 catacgcctg actgccggta tggcgcgaag aaataatgtt gtcgacggaa taaatctcgg
     5281 tatgcccgtc ctgaatgcgc atcgggcgca gcagaaattc gttttccagt ggcgaaaggt
     5341 gtaacggatc ggcttccagc ggaaacaggt tgatgaccgg cacgcagtgc agacggatat
     5401 tatcgctgtc aaacggcagg tcgtgaggcc agttctcgtt cagcaacacg tcaatctcaa
     5461 agccggttat cccctccggc catgcaacct gctccagccc gttcagcgcc acgaacataa
     5521 acttctcgcg gaaggtgaaa tactccagca gcaactggta gccgctgaac gccgattcgc
     5581 ctttcggcca cagtcggtca tcgtcatcaa aacccatcgg ggaaaagtgc gcgtccagca
     5641 cacggcgtcc ctgccctggc agacgcagcc agaactgctg cgtaccaagc gttaaagccc
     5701 ggtgcagcgc acaggccacc gggctgtcgg cactgaggta cagcggcagg cgggataaat
     5761 caatctgtga ccagtcacca accagcgggc cgcattcaaa gcgcagacgg ataaccgagc
     5821 gtccgtccgg ttcatattgc aatgacacgt ccggcaacgc cagcggatgc agcgtgatat
     5881 cccgcgtggc gctgtagacg cagcgggttc tgcgttcgcc gatgggacgg gatagcacct
     5941 gaaactcttt cagcgtatca ctttgcttca tttgttggtg atccgtggtc agttcgacca
     6001 cggaaagcga cggaatagtg cgcaggtagt gcggccacag caggctgacc agcccttcag
     6061 tcagttccgg cagatcgtcg tccagctttt cgcgcaggcg gcccattaaa aaagcgaagc
     6121 cctcgaacag gcgctccaca tagggatcgc gtgcgccggg tttatcaaga ttaagcatcg
     6181 cagcgcggtc ggggtaagcc tgcgcaaatt ctttccccgc ttcacgcagg tagcgcattt
     6241 cggcctcaaa gtagcgttgg gtcaggtcgt ccatcggagg atttatcttt ccttttattg
     6301 aaattaatac caacgagtag tgagattaga attttttatt ttcagaatcc taaggacata
     6361 tagatggatc atttatttca aaagaaaa
//
LOCUS       DAPQZD010000231         6069 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPQZD010000231 DAPQZD010000000
VERSION     DAPQZD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.233"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            164..811
                     /gene="fucA"
                     /locus_tag="RO454_004604"
     CDS             164..811
                     /gene="fucA"
                     /locus_tag="RO454_004604"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDY0936327.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            839..1987
                     /gene="fucO"
                     /locus_tag="RO454_004605"
     CDS             839..1987
                     /gene="fucO"
                     /locus_tag="RO454_004605"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDY0936328.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2092..2847)
                     /gene="xni"
                     /locus_tag="RO454_004606"
     CDS             complement(2092..2847)
                     /gene="xni"
                     /locus_tag="RO454_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDY0936329.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2959..4326)
                     /gene="sdaB"
                     /locus_tag="RO454_004607"
     CDS             complement(2959..4326)
                     /gene="sdaB"
                     /locus_tag="RO454_004607"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDY0936330.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4384..5673)
                     /gene="sdaC"
                     /locus_tag="RO454_004608"
     CDS             complement(4384..5673)
                     /gene="sdaC"
                     /locus_tag="RO454_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDY0936331.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1531 a         1494 c         1619 g         1425 t
ORIGIN      
        1 ttgcagttat ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct
       61 ttctggttaa tcaggaagaa tatggtgagt aattaaacgg ctaattcaat agtgtgaaag
      121 gaacaacatt attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact
      181 tgctcgtcag attattgaca cttgcctgga aatgactcgc ctggggctga accagggaac
      241 agcgggtaac gtcagtgtac gttatcagga tgggatgctg attacgccta caggtattcc
      301 atatgaaaaa ctgacggagt cgcatattgt ctttattgat ggcaacggca aacatgagga
      361 aggaaaactc ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga
      421 tgccaacgcg gttgttcaca atcatgccgt tcattgtacg gcagtttcca ttcttaaccg
      481 accgatcccc gcgattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc
      541 gccttatgcg acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa
      601 tcgtaaggca actttgttac aacatcatgg gcttatcgct tgtgaggcga atctggagaa
      661 agcgttatgg ctggcgcatg aagttgaagt gctggcacaa ctttacctga cgaccctggc
      721 gattacggac ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt
      781 caaaacctac gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat
      841 ggctaacaga atgattctga acgaaacggc atggtttggt cgaggtgctg ttggagcttt
      901 aaccgatgaa gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct
      961 ggtgcaatgc ggcgtggtgg cgaaagtgac cgacaagatg gatgccgcag gactggcatg
     1021 ggcgatttac gacggcgtag tacctaaccc aacaattact gtcgtcaaag aagggctcga
     1081 tgtattccag aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga
     1141 tacgtgtaaa gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct
     1201 ggaagggctt tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc
     1261 aggcactgcg gcagaagtga ccattaacta cgtgatcact gacgaagaga aacggcgcaa
     1321 gtttgtttgc gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat
     1381 ggatggtatg cctccagcgc tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat
     1441 tgaggggtat attacccgtg gcgcgtgggc gctaactgat gcgctgcaca ttaaagcgat
     1501 tgaaatcatt gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga
     1561 aatggcgctc gggcagtatg ttgcgggtat gggattctcg aatgttgggt tagggttggt
     1621 gcatggtatg gcgcatccac tgggcgcgtt ttacaacacg ccacacggtg ttgcgaacgc
     1681 catcctgtta ccgcatgtca tgcgctataa cgctgacttt accgatgaga agtaccgcga
     1741 tatcgcgcgc gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc
     1801 cgccgttgaa gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttacgtga
     1861 tgttggtgta cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg
     1921 taccggtggc aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc
     1981 ctggtaaatg tgctgatgtg ataatgccgg atgcgacgct agccgcgtct tatccggcat
     2041 ataagtgcat taaattcaat gtgttgataa tcgtttgcat ccggcacgcc gttaccttac
     2101 caaccgcaat tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc
     2161 gcggcacaga aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac
     2221 cgcatccaga ttctcatata tcccttccag actctgaaac tcgaccagta gctgcgtggc
     2281 gctttttggt ccgatccccg caacacccgg tacctttgaa ctgctgatcc ccgcaagccc
     2341 ccagtaatcg ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc
     2401 cagccaacgt ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta
     2461 gcctttatct gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag
     2521 cgtggcggct aagtcatcgg cttcgttgcc gctggcggac cagcacggga cgccgcgttg
     2581 ctcaaaggcg gcgcgtaatg caggcatctc gttgtgcaac tcttccggca ttggcggtcg
     2641 acccgctttg taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc
     2701 aaaaacggcg accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcgtgctg
     2761 gcaggtttcg acacagggcg aaccctgaac ggcatgaatg cgacgaataa gattcagtgc
     2821 atcgacaata agcaaatgaa cagccacgat aaccccctta aagatcaggc gataagggta
     2881 acattcgatg ccagaacagg ctatggctgg atgagaaatc gggaagaggc ctcgcaaaaa
     2941 gaggcctctg gagagcgatt aatcgcaggc aacgatcttc attgccaggc cgccgcgaga
     3001 ggtttcgcgg tacttggcgt tcatatcttt acctgtttcg tacatggtct cgataacttt
     3061 atcgaggcag acgcgcggct cgctggtacg gcgcagcgcc atacgcgcgg cgttcaccgc
     3121 ttttactgcc gcaatggcgt tacgctcgat gcacggtacc tgcacctgtc cagcaaccgg
     3181 gtcacacgtc agaccgaggt tatgctccat ggcgatttcc gccgcgatgc acacctgcgc
     3241 cgggcttgcg cctaacagtt ctgccagacc agctgccgcc atagagcacg ccacgccgac
     3301 ttcaccctgg cagccgactt cggcaccaga aatcgaggcg ttcatcttat aaagagaacc
     3361 aatggcgctg gctaccagca ggtaacgcgc cagtgagtta gcgttcactt cgcggataaa
     3421 cttgtcgtag tacgccagca cggccgggat aatcccgcac gcaccgttag tcggcgcagt
     3481 caccacgcga ccgccagcag cgttctcttc gttcactgcc agtgcaaaca tgttgatcca
     3541 gtcaactacc gccatcgggt cagtggtggt tttatcctgg ctgaccagca tccggcgtag
     3601 tgccgcagca cggcgtggaa cgcgcagttt gccaggtaac acgccttcgg tagaaatacc
     3661 gcgttcaata ccgccacgca tgacttccca gacgttcgcc aggtgctgtt ccagctcttc
     3721 tttgctgtgc agcgccagct cgtttttcat catcaggcca gagagtgaca gcccggtttc
     3781 ctgacaatgt ttttgtagat cggcagcgga actgtacgga taaggaactt caaccggtgc
     3841 gctatcctgc tgaccaaaat gctcttcatc aacgataaag ccaccgccaa tagagtaata
     3901 agtctggctg taaacgactt tatcgcccgc cagcgcggta atgcgcatac cgttttcatg
     3961 cagagaaagg ttgtcggcat ggaagttcat gcactgatca accgggaact ccacttcatg
     4021 ctgaccgttt gccagcatca ggcgaccatg agtattcaca tcctgaataa aaccagggat
     4081 ggaatcgata tccacggtat ccggcaggtt acccgccaga cccataatta tggcgatatc
     4141 tgtgtggtgg cctttaccgg tcagagagag cgagccgtac acgtcaacca ccacgcgggt
     4201 cacgtcttta agcaggttac gggcaatcag atcgtcggta aattgtttac ccgctttcat
     4261 tggtccaacg gtatgagaac tggaagggcc aatgccgatt ttgaaaatat cgaatacgct
     4321 aatcatagga aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag
     4381 gacttagctg aacagagagt agaagattgc ggagattgca atcagaccca tcacgacaac
     4441 gaatacgttg ctgatgtgac cgctgtactt acgcattgcc ggtactttct gaattgcgta
     4501 catcggcatc aggaacagga tcatcgcgat gattgggccg cccagggttt caatcatacc
     4561 caggatgctc gggttcaggg tggcaacaat ccaggtcgtt accagcatga acagcgcagt
     4621 gatacggttc agcttgttga tttcgataga cttaccttta ccacgcagag atttaatcac
     4681 cataccgttg aagccttcac gtgcgcccag gtagtggccg aggaaggatt tggtgatagc
     4741 gataatcgcg ataatcggag ccatccacgc gatgatcggc gcgttaaagt ggttagccag
     4801 gtaagacaga atcgagatgt tctgctcttt agccgcagcc aggtctgccg gagtcaggct
     4861 cagtacgcag ctgaagacga agaacattac ggtcagcacc atcatgatgt gtgcgaatgc
     4921 caggatctta gagcatttct gttctgccat atcgccgtac tcttcacgct tcgcaacggc
     4981 gaaagaagag atgatcggag agtggttgaa cgagaacacc attaccggaa ttgccagcca
     5041 cagggtcatc cacagaccgt ttccggttgc agatgcagtg tccagagaca gcgtttccag
     5101 tgctgcgccg ttccactgcg ggatcaggta cagagccagc agcatcagta cgccaacaaa
     5161 cgggaatacc agaatactca tcgctttaac gatcatctgc tcaccgaagc gaacgatggt
     5221 catcataccc acgatcagga tcagcgacag aatcgcacgt ggcggtggcg tcatacccag
     5281 ttggtgagac atgaagcttt caactgtatt ggtgattgcc acgctataaa ccagcaggat
     5341 cgggtagata gcgaagaagt agagcagagt aatcagttta cctgcgccaa taccaaagtg
     5401 ttcttctaca acctcggtga tgtcttcgcc cgggttttta ccagacagta cgaagcgagt
     5461 caggccgcgg tgagcaaaaa acgtcatcgg gaatgcaagg atagccatga tgatcagcgg
     5521 gatcataccg ccaacaccgg cgttgattgg caggaacagc acgcccgcgc cgattgccgt
     5581 gccgtaaagg cccagcatcc acatggtgtc tgtcttgcgc caggcactac gagagtcttt
     5641 cgacgcaatc gtgctggttt gagtcgtttc catctatttc tcctggagga atgcaaataa
     5701 ttcagctttt agccagttca gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta
     5761 tcggcgatgg cttaatcatt ttccaccgta ataatttgag ggcggaaaga tacacacaag
     5821 ttatgtatct atcagtgatc ttgatcccaa atacaataat ctacttggaa gatagataat
     5881 cacaacgctt tactctgtaa atcaaaagta aaagagaatt taagggcaat atttgcccat
     5941 taaacgacat ctgacttctt ctggggcgcg caatatatgt aggttgaact ggaaaaatca
     6001 atacttttag ttaatcatgg cgataatttc tttttcatgg cgataaaatg tggtttatga
     6061 aaataggga
//
LOCUS       DAPQZD010000232         5807 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPQZD010000232 DAPQZD010000000
VERSION     DAPQZD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.234"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..131)
                     /locus_tag="RO454_004609"
     CDS             complement(<1..131)
                     /locus_tag="RO454_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDY0936332.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     "
     gene            complement(434..1027)
                     /gene="tehB"
                     /locus_tag="RO454_004610"
     CDS             complement(434..1027)
                     /gene="tehB"
                     /locus_tag="RO454_004610"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDY0936333.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1024..2016)
                     /gene="tehA"
                     /locus_tag="RO454_004611"
     CDS             complement(1024..2016)
                     /gene="tehA"
                     /locus_tag="RO454_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDY0936334.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLCLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2140..3120
                     /locus_tag="RO454_004612"
     CDS             2140..3120
                     /locus_tag="RO454_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDY0936335.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            complement(3112..3651)
                     /gene="rimL"
                     /locus_tag="RO454_004613"
     CDS             complement(3112..3651)
                     /gene="rimL"
                     /locus_tag="RO454_004613"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDY0936336.1"
                     /translation="MTETIKVSESLELHAVAESHVTLLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(3714..3938)
                     /locus_tag="RO454_004614"
     CDS             complement(3714..3938)
                     /locus_tag="RO454_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDY0936337.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(4078..5733)
                     /gene="opgD"
                     /locus_tag="RO454_004615"
                     /gene_synonym="mdoD"
     CDS             complement(4078..5733)
                     /gene="opgD"
                     /locus_tag="RO454_004615"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDY0936338.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT         1572 a         1419 c         1356 g         1460 t
ORIGIN      
        1 gtttctttta aatcagagta attgtttaaa aaaccagaat atttatctgg ctgggtgatt
       61 ttagaggcac aaccagacag agccaacaag ccgcataaag ctgcaacttt cgcaaatgat
      121 gtggtacgca tgaatagttt cctgagatga ttatattact ttttaagtta tagcaaaaag
      181 cgccgcttcg tgttgtggca aaaaaagtgt tggtgaaaat aaatttttta gccccttttc
      241 agacgacata gtcaatgccg aaaaaggggc taagtcaatc atcagtagtt aaatttcagt
      301 acgttaagcg gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa
      361 atatgcttac cgaaaagaag aacagtaaac catctcagcg tggggaattt tcacgcgctt
      421 tgttcttacc ggatcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat
      481 taccgttggc gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accatctccc
      541 agccttcgta atatcgacgt aattccccct ctttgaaggc aaacgggaag ccgacggtac
      601 atggataatc agcggtatcc atcgccgcca caatcaggtt gtaaccgcca ggtttagcgc
      661 aacgttgcat attggcaatc agccctggga tggttttagc ctcaaggaac atcagcacca
      721 cagtcgaaag aataaaatcg tactgtccat caaatgtgag gttattcaga tcaacgaccc
      781 gggtgtgtaa attatccaga ttttcaatgg acttaatgcg ctcgacgttg gcgatactca
      841 tggcattttt atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac
      901 gaccattgcc acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca
      961 gcacttcaga gtgtgtgcgg gttaattcat atttatcagt aaaatagttt tcatcacgaa
     1021 tgatcattct ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt
     1081 tccctgcatc agaagcgcaa aagtacggat gagcagtatt gcaataataa aattggtaaa
     1141 gataaacagc ggcaccgcca gcgtatggaa aaatccatta tcgctgccac tccccagatg
     1201 caaaccggtg gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa
     1261 tggctgggag agataccatg gcatcagaca tagcataaac agcagttgca gcagtccata
     1321 accgaaaagc attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca gccaggcact
     1381 acaggccacc agcgcaggag cgagctgaat gccgagtgat gtcctcagtg ccgtgggtaa
     1441 ttctcccgaa ctgcgcagac gctgcaagat caccggttcc aggcttagcc atgagaaaac
     1501 gcctgcgcct aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc
     1561 gctgataaag ttgttggcaa ctgtcggcag atacagtcca ggcgtggtag cttcttcagg
     1621 gtgagatcca cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac
     1681 accaaaactg aacaggcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac
     1741 cagcatcgtc gttgccggaa acaaactcac aaaactgctc agcactggat ggcgcacttc
     1801 tgccagcacg ctatgcggaa agcgtatgag tcgggtaata aatgcgccag tcaataatcc
     1861 ccagatgatc atcgccagaa tcaccagccc atcccccaac cagtggctga cctgccaaac
     1921 ctggctggca tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta
     1981 gcctgccggc aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt
     2041 ataaagtata tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa
     2101 tttgctacgg ttatagtaag tcacctgtgg gaaaacgcta tgcgtaaata tcgtctaagt
     2161 gaagaacagc gagcctttag ttatcaggaa gatggcacta aaaaaagtgt gttattacgg
     2221 caaattatcg ccatgagcga ttttaacgat gtgatagcag gaagcgctgg cggctggatc
     2281 gatcgcgaaa cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt
     2341 gcgttcggcg gaacggtgat ttccgggaat acgcgtatca ccggcaccag cgtgctgtgg
     2401 ggagaggttt acgcaacgga taatgtctgg atcgataata gtgaaatcag ccagggtgca
     2461 tatataagtg acagtgtcac cattagtgac tcattagttt gcggtcaatg tagaatattt
     2521 ggtcacgcct taattgatca acattctatg atcgtcgccg cacaaggctt aacgcccgac
     2581 catcaactcc tgctacaaat ttatgatcgc gcaaaggtta gcgcctcgcg tattgtccac
     2641 caggcacaaa tttatggcga tgccgtcgtc cggtatgctt ttattgaaca tcgcgccgaa
     2701 gtttttgatt ttgccagtat tgaaggtaat gaagagaaca acgtctggct atgcgattgc
     2761 gctaaagttt acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccgacg
     2821 attcattaca gttcacaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa
     2881 catcatgtct tgattggcgg aaatgccgtg gtacgtggcg gaccgattct gctcgacgag
     2941 cacgttgtaa tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg
     3001 gagttaactg accacgccgt cgttgaagcc tttgatggcg acacgattca tgtccgtggt
     3061 ccaaaagtca ttaacggcga ggagcgcatt acgcgaacgc tgctggcagg gttattgtga
     3121 atcgataata cgcgcgtata agttcacatc atcataggca tcattcagaa actcagcctg
     3181 tttcaggcaa ccttcaagga taaaaccatt gcgcaacgct acctggttgc tttgcggatt
     3241 gtccacccga catttgatca cgaagcgtct aagttcacca gactgagcgt aatgatgaat
     3301 caatgcctgc agcgcctgag aaatgatccc ctgcccctga tgaggttcgt ccagccagta
     3361 gccgatttca gcggttttat tcagtggttc aatacgatta aaagagataa cgccgataag
     3421 ttcatcttct ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt
     3481 accctgcacc gtttttcgcg tgtcctcttc actttgaaca aactgcggcc agtttagcga
     3541 ctgctgtaac caggttttat ttttacagat taactgataa agaagtgtga catgactttc
     3601 cgcaacagca tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc
     3661 agtcaatcca tgcagctgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc
     3721 tctttgacgc tcacctgctg caggattttg agcatctcat cttttaactg tagcttttgt
     3781 tttttcatgc ggaccacttc cgcattgtac cctcgaccgt cggaaccttc ctttctggca
     3841 atttcatgat caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt
     3901 ttcagacggg atattaaatc tcggtattct ggaaacatac gcactccctg ttagtggctg
     3961 acaaggtgta tctgaaatac acaccagcag attactcatt taacttagga aaaaatgcga
     4021 gcgggatcgt gtttaaccag acggcacctc ccgaagaaga tgccgaagaa aaaacgatta
     4081 actcatcacg cggtcatcaa catactgacg tttatccggc gctggcggga aatactgata
     4141 taaccatgtt tcactgatag cgtcgccctg acaacgcaga tacatccgca tatcgaccgg
     4201 atcagtggag tccgaagtcg gataccagtc aaactgaata cgataaccat caatgggttc
     4261 aatatagaga atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat
     4321 gccttttggc gcggcagctt tcagatcgcc accaacgaaa tcgacggcaa aacgtcgcgc
     4381 ccatttttcg ggatagtgtt cacctggcgc ccaaccttcc gggaaaccgc ccatgccggt
     4441 acgcgtcgcc ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag
     4501 acgatactgg aatgcaaact catcacccgc ttttacagct ttttctggct gccagaagca
     4561 aacaatgtta tccagcgttt cccccgtagt tgggatttcc atcaggccga tggtgccctt
     4621 accccacttg ttacgcggtt ccacccacag acttgggcgt ttgttatacc agcccataat
     4681 gtcctgataa tgggagaaat cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt
     4741 gtcggtgtaa gcattgaact gcaatttttg cggattgttc agcggacggc aaatccactc
     4801 gccgttgccc cgccacatgg acagacgatc agagtcatgg atttgcggat gaatggtgtc
     4861 gcacatccga cgttcattag taccgcagct gaacatactg gtcatcggtg caatgcccag
     4921 ctgtttaatg tctttgcgcg catacaggtg attttccaca tccataatca cctgattttt
     4981 ctcacaatgg atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata
     5041 aacggtaaag gtggttgccc ctggttttac cgtatcaaac cagaaggcgg taaagtcggg
     5101 gaactcttct ttgctgtcgg tgtaagtgtc gatcgccagg ccgcgggccg acaaaccgta
     5161 ttgatatgtg tcatcaacgg cgcggaaata actcgcgccg aggaatgata ctacatcacg
     5221 gcgcgccagt tcgggggctt taaacacgcg aaaaccggca aaaccgagat cgctttgccc
     5281 ttctaattgt ttggtatcca cacccgcatc gttgtatttg aacaactccg ggcgaaagtg
     5341 aatttcacgc gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa
     5401 tcccattccc atatggaaaa actgagcgtc cagttgacgg ttctcaacgt tatgccagag
     5461 cgatttttcg gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagagt
     5521 atcaggtaac ggacgtggtg caccacgcca tgctgtttgc gctaagtcgt gcgccattga
     5581 ctgtagaatg gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatcggccgc
     5641 gaatgccgcc tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc
     5701 cattgaacct ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg
     5761 tgaatggtcc cgcgctggtc attttcagcg gcaagaaagt gcttatt
//
LOCUS       DAPQZD010000233         5776 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPQZD010000233 DAPQZD010000000
VERSION     DAPQZD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.235"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(334..1293)
                     /gene="nrdF"
                     /locus_tag="RO454_004616"
     CDS             complement(334..1293)
                     /gene="nrdF"
                     /locus_tag="RO454_004616"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDY0936339.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1303..3447)
                     /gene="nrdE"
                     /locus_tag="RO454_004617"
     CDS             complement(1303..3447)
                     /gene="nrdE"
                     /locus_tag="RO454_004617"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDY0936340.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3420..3830)
                     /gene="nrdI"
                     /locus_tag="RO454_004618"
     CDS             complement(3420..3830)
                     /gene="nrdI"
                     /locus_tag="RO454_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDY0936341.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDESYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3827..4072)
                     /gene="nrdH"
                     /locus_tag="RO454_004619"
     CDS             complement(3827..4072)
                     /gene="nrdH"
                     /locus_tag="RO454_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDY0936342.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4319..4648)
                     /locus_tag="RO454_004620"
     CDS             complement(4319..4648)
                     /locus_tag="RO454_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDY0936343.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4800..5144
                     /locus_tag="RO454_004621"
     CDS             4800..5144
                     /locus_tag="RO454_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDY0936344.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5181..5630)
                     /gene="alaE"
                     /locus_tag="RO454_004622"
     CDS             complement(5181..5630)
                     /gene="alaE"
                     /locus_tag="RO454_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDY0936345.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1460 a         1425 c         1441 g         1450 t
ORIGIN      
        1 ttgtttatgt cgatacatta cccaatggaa atagtcactt ttttctaccc taacatactg
       61 agaatctgag gcaacccctg atggcaaatg tggcatgaat attgatgtga gcaaatcgcc
      121 ggaatttccc atgatataag ggctgagagc aaatcgagaa aagttgatat tttttgtcaa
      181 agatagcaaa aaaacgcctg aatattgtgg tttcaggcga ataatgcaac agagtatcag
      241 tgtagatcac cacaaattat ttgcgtgata taaagctatt tggcgggaat tatttccctg
      301 cagcgggtag tgatattttt gaaaataacc ccctcagaaa ttccagtctt catcttctgt
      361 ttcgaccgct tttcccatca catatgaaga gcctgaaccg gaaaagaaat cgtgattttc
      421 atcggcattc ggcgaaagcg cggcgaggat tgccggattc acttccgcca tttctgcggg
      481 aaatagcggt tcgtagccca gattcatcaa cgccttattg gcgttgtaac agagaaacgc
      541 tttcacatcg tcagcccacg gggtttcggc gtacagctca tcggtgtatt gcaactcgtt
      601 gtcatagagt tccagcaaca aatcgaaggc gaaactcttc aattcttcac gtcgtgcctg
      661 ggagatcttt tccaggtttt tctgatattt atagccgatg tagtaaccgt ggactgcttc
      721 atcccggata atcagacgga tcaggtctgc ggtattggtt agctttccgc ggctggaaaa
      781 atacatcggc agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat
      841 tttctttttc agcggatcat caccgcgata atgttgctga ataatctgtg cttttcgctg
      901 caacggcgcg ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctggcacag
      961 cgtcgagaaa attgaactgt aagagcgggc atgaaccgct tccataaagc tgatatttga
     1021 taataccgcc tcttcatggg gggtgagtgc atcgggcatc agcgagggcg cgccgataac
     1081 attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg cgcattgtta gttgttgttc
     1141 tacgacactt aatgtctgcc atgcaggaat atcgttcgac agtggcacct tttctggtag
     1201 ccagaaattg ctggtcaggc gattccacac ctccagatct ttatcgtcag atatcttgtt
     1261 ccagttgatg gcgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac
     1321 gcagccttca atttcagtgc cttccagcgc catctgacgc aggcgaatgt aatagagcgt
     1381 tttgataccc ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag tggtggcggt
     1441 atcggggaaa aacagcgtca gcgacagccc ctgatcgaca tggcgagtcg cttccgcgta
     1501 ggtgtcgatg atcttttctg cgccgatttc gtaagcgtcc tgatacagcg ccagattttc
     1561 gttagtcata aacggggcag ggtagtaaac gcgcccggtt ttgccctctt tgcgtatctc
     1621 tactttcgcc acaatcggat gaatactcga cgtggcatgg ttgatataag agatagaacc
     1681 ggttggcggc accgcctgga gattctggtt gtagatacca tagcgcatca cgtcgtcgcg
     1741 cagctgcgcc cacatctcac gggtaggtaa cgtaataccg ctacgggcaa acagttcgcc
     1801 aactttcgcc gttttcggct gccagttact ttgcagatat tggctgaaat attcaccact
     1861 ggcatagcgt gactgtttga acccggcgaa ggtttcaccg cgttcgcgcg ccagcaacat
     1921 cgaggtacgc agtgcgtgcc aagtgatggt atagaaatag agattggtga aatcgagtgc
     1981 ttccggcgaa ccataagcga tgccttctcg cgccagatag ccgtgtaaat tcatctgccc
     2041 cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg
     2101 actcatatct gacaccgccg ttaaaccgcg tacggcagtc tctaccgtgc gggcaaagtc
     2161 gggggaatcc atggtgtggg caatattcaa cgaccctaga ttgcaggaaa tatcatggcc
     2221 tgtacgggca tagtcgagat tctcgtcata ttctgaggcg ctgttaacct gcaaaatttc
     2281 tgagcagaga ttgctcatat ttatgcgccc ggcgataggg ttagcacggt taaccgtgtc
     2341 ttcatacatg atgtacggat agccagactc aaactggatc tctgccagtc gctggaagaa
     2401 atcacgagcg ttgaggtatt ttttgcgaat gcgttcatcg gcaaccagtt cgtcatagcg
     2461 ttcgctgatg gcgacatcgg caaacggttt gccataaacc cgctcaacgt catagggcga
     2521 aaacagcgcc atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac
     2581 gcccagcgac agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt ttgtatcgag
     2641 aaaacgcaga atatcgggat gatgagcatg taaatagact gccccagccc cctgacgcgc
     2701 gccgagttgg ttggcatagg aaaatgcgtc ttccagcatt ttcatcaccg gaatcacgcc
     2761 agaagattga ttttcaatac gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa
     2821 aaatgctacg ccgccgccgc gtttcgacag ttgtagtgcg gaatttactg cccggccaat
     2881 cgactccata ttgtcttcaa tacgcagcag aaaacaggaa accagttcgc cgcgctgctg
     2941 cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag cgtcctgaca gcatctcatc
     3001 ggtgagctgc gtcgccagtg tttcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac
     3061 gcgatcggca aaatcttcca gataacgctt accgtcgaat gtcttcaacg tatagctggt
     3121 gtaaaacttc catgctccta ggaatgtctg gaaacgaaaa ccgctggcgt gcgcgtgggc
     3181 aaacagcgta atgacaaaat cgcgagagta gcgattaagg acactttcat catagtaacc
     3241 ttcgttaacc agccagttca ggcgctgctg ctggctactg aaggccacac tgttcggacg
     3301 cacatgcgtc gtcataaagg cgtctactgc ctggcgatct ttatcgaact gaatgcgacc
     3361 tgcgctatcg taaaggttaa gcatcgcatt cagagcgtga taatccatcg tttcctgcgt
     3421 caggcgttct gcggttgtcg ttgccaaaat tcagttactc ctttacgaac gttttcgata
     3481 tcgctttgcg tacccatgag ttcaaagcgg tacagccacg gcacgccgca tttccgggca
     3541 atcacatctc cggcgcggcc atatgcctca ccaaagttgc gattacccga agcaataacg
     3601 ccgcgcagca acgcccggtt atgctcgtcg tttaaaaagc gaatgacctg tcgtggcacc
     3661 gcgccagccg taccgccgcc gccgtaagag ggcacgatca ggatgtaaga ctcgtctacc
     3721 tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg gcagccctaa acgttcgata
     3781 aaacgctgcg tattttcgga gctgctggaa aagtagacga gctggctcat gcactggccg
     3841 cgtgtggcgc tggatgcaga cgattgatca tgtccgggcg gaaaccagac cagctaagat
     3901 cgcctgcaat cacaaccggc aactgacgaa agccctgagc acgcagcgct tctgccgctt
     3961 caggaacgcg atcgacatta atcatttcaa aatcaaagcc ccggttttcc atcgcccgtt
     4021 tagtggcgtg gcactgaacg caatcgttac gagtgtaaat agtaatgcgc atgattcgta
     4081 tttccgttta aaatgaagat acggcgcgat gatacgcgtc gggttgtctc tctgttgata
     4141 cagagatact agatgtagtt gaaaaaagat tcaaccacac aatatatagc aaataatggt
     4201 cagggttacc ctggggcgag cgtgacgggg tagggggatt tttgtgattc accaggcaaa
     4261 aagaaacccc gaagccaggc ttcggggtca aagacgcgta tttattatca tttttgcact
     4321 acgatttgcg catgcttaac agtgcgccga taaaaatacc caccgctgcc gctgtaccca
     4381 cactacacca gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg
     4441 cttgctggac gcgagtacga ccatgcattc gcgccctggt ttctttcagt agagcctgcg
     4501 ctttactacg agcggcttcg gcttcccctt tggcgtcgct gccccaggac ttcaatacag
     4561 attcgagact gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa
     4621 cgtcgttgcg gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc
     4681 agtgtagacc ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag
     4741 cgttgtgaat gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata
     4801 tgtatttacg accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg
     4861 tggtaaccca gaaagcgtat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg
     4921 aagcgcgaat gggacgtacc gccctggtga ttcatccaac attaaaagaa cgtagttcaa
     4981 cactggctga acccgcttcc gatattaaaa cttgcgatca ttaccagcaa tttccgctct
     5041 atttagcggg tgaacgtcat gagcattacg gtatcccgca tggctttagt tcgcgtgttg
     5101 cgcttgaacg ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat
     5161 gccagtcgcg aaaagaaggg tcaggctttt acctgctggt aacggctgac tttaaacagt
     5221 cggcggcaat aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg
     5281 tttgaactga ctgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc
     5341 acatacaccg gtgactggaa cgtcacataa gccaggatat ccgccagatt ttttatccag
     5401 cctgacgggc taactttgcg tgccgcgcgc ataaacagat cgcggtacat accgtatggc
     5461 catgcaatta agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag
     5521 ctcattccgg agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc
     5581 gcgaacgtat ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc
     5641 cttaataaaa gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct
     5701 gcatttttgc atagcgtata tcgctggcta tgactaatca attagtgaaa aattttcatt
     5761 taatctgttt ttgtag
//
LOCUS       DAPQZD010000234         5762 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPQZD010000234 DAPQZD010000000
VERSION     DAPQZD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.236"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            135..845
                     /locus_tag="RO454_004623"
     CDS             135..845
                     /locus_tag="RO454_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDY0936346.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPLIKATMQ"
     gene            887..3538
                     /locus_tag="RO454_004624"
     CDS             887..3538
                     /locus_tag="RO454_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0936347.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            3552..4082
                     /locus_tag="RO454_004625"
     CDS             3552..4082
                     /locus_tag="RO454_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0936348.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHTGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4095..4598
                     /locus_tag="RO454_004626"
     CDS             4095..4598
                     /locus_tag="RO454_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0936349.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYIWQ"
     gene            4658..5572
                     /locus_tag="RO454_004627"
     CDS             4658..5572
                     /locus_tag="RO454_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936350.1"
                     /translation="MGKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVILPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
BASE COUNT         1658 a         1250 c         1265 g         1589 t
ORIGIN      
        1 aagagaacca acatttatta caaacgcatt aagtctggaa acccggcgac tcgccaatgt
       61 attagcaata tttaccgatt gtctgatgta acggtttccc cgcttatcct cttaacacat
      121 ggagaatgca tcagatgcaa actacgcgta ccccctactc tgtttccttt atggcgacag
      181 tgttactttt atttctcttt gcttgccata gcaccattgc caacgcagct gtcgcacttg
      241 gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt tttattgcct gtcacgaata
      301 atgatcctgc cagtgtctat ctcattcagt catggataga aaatgcgggt gaccagaaag
      361 atacccagtt tgtcattacc ccaccgcttt tttccatgca aggtaaaaaa gagaatacgc
      421 ttcggataat caacgccact aatcatcagt taccaggcga ccgtgaaagc ctgttctggg
      481 ttaatgttaa agctatccct gctatggaaa aagaccagaa gaatgagaat acattacagc
      541 tggcaattat tagccgcata aaaatgtttt atcgccccac gcatctggca atggcacctg
      601 aagaagcgcc tgcaatgctt cggtttcgtc gcagcggtag catactcact cttattaatc
      661 caacgcccta cttcattacc gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca
      721 ccatggtgcc gccaaaaggt gaagtatcgg tggatatccc gcatgctgta accggggata
      781 tcagttttca gacaatcaat gattacggtg cattaactcc gcttattaag gcaaccatgc
      841 agtaagccag tgacctaatt aataataaac tcaatcggga ttaaaaatga cagcctttcg
      901 tgccgcattt aaggcatatc gtatgcatca ggtgttaata ctgcctcgct ttgcacggct
      961 tacctttgcc cttggcttag caactgcggt ttttcccgtt gacgcagagt attattttaa
     1021 tccacggttt ttaagcaatg accttgctga atctgtcgat ttatctgcct ttactaaggg
     1081 acgcgaggcc ccacctggta catatcgggt cgacatttat ttgaacgacg aatttatggc
     1141 tagtcgggat attactttta ttgcagatga taataacgca gatctcatcc catgcctgag
     1201 tacagacctt cttgtcagtc ttggcataaa aaaatcagca ttattagaca acaaggaaca
     1261 ttcggcagat aaacatgtgc cggacaacag cgcatgcaca ccacttcagg atcgtttggc
     1321 tgacgcatca tctgagtttg atgttggtca gcaacatctg tcactctccg ttccgcaaat
     1381 atatgtcggc agaatggctc gcggctatgt ttctcctgat ctgtgggaag aaggaataaa
     1441 tgccgggcta ctaaactata gttttaatgg taattctatt aataaccgta gtaaccataa
     1501 tgcaggaaaa tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg
     1561 gcgactgcgc gataactcaa cgtggagtta taacagtggg agtagcaatt catctgacag
     1621 caataaatgg cagcatatca atacgtcggc tgaacgtgac attattccgt tacgctcacg
     1681 gttaacagtg ggtgatagtt ataccgatgg cgatattttt gacagtgtga actttcgtgg
     1741 ccttaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt
     1801 gattcatggt attgctcgtg gcaccgcaca agtgagtgta aaacagaatg gatacgatgt
     1861 ttatcagact actgtcccac ccggcccttt tactattgat gatatcaact ctgcggccaa
     1921 tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt
     1981 tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg cacttgccat
     2041 gggggaatat cgtagtggga ataacctgca aagctccccc aagttcgtac aagcaagctt
     2101 gatgcatggt ctgaaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta
     2161 tcaggccttc aaccttggta ttggtaaaga tttaggactt tttggtgcct tttctttcga
     2221 tatcacgcag gccaatacga cgcttgcaga tgacacccgt cacagcggac aatcggttaa
     2281 atccgtctac agcaaatcct tctaccagac gggaaccaat atccaggtcg caggatatcg
     2341 ctattctacg caaggttttt ataacttatc cgacagtgca tacagtcgaa tgagtggtta
     2401 caccgtcaag cctcctaccg gagacaccag tgagcagaca ctatttattg attattttaa
     2461 tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggacatta
     2521 cggtacgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca
     2581 gcaaatatca tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag
     2641 ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta cacttaatgt
     2701 tccctttagt cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag
     2761 ttacagtatg tcaaacgatt tgaaaggcgg catgaccaac ctatcagggg tttatggcac
     2821 tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc aagggggtaa
     2881 tacatcgtct ggcaccagtg gttacagttc tcttaattat cgtggagctt acggtaatac
     2941 gaatgtcggt tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg
     3001 gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca caatggttct
     3061 ggttaaggct cctggcgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga
     3121 ctggcgtggc tatgccatat taccatttgc gacagaatat agagaaaatc gtgtcgctct
     3181 taacgcgaat tcccttgcag ataatgttga actggatgaa accgtagtca ctgtcatccc
     3241 aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat
     3301 gacgttgaag tacggtaata aaagcgttcc attcggtgca attgtcactc acggagagaa
     3361 taaaaatggc agcattgtcg cggaaaatgg tcaggtttat ctgactggac ttccacagtc
     3421 agggaaatta caggtttcat ggggcaatga taaaaactca aactgtattg tcgattacaa
     3481 gcttcctgca gtctctcctg gaaccttgct gaaccagcag acagcaatct gtcgctaagg
     3541 aacaatagat tatgaaatac aataacgtta ttttcctcgg tttatgtctg gggttaacca
     3601 cctattctgc tttatccgca gatagcgtta ttaaaattag cgggcgcgtt ctcgattatg
     3661 gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca
     3721 gacaatttcc aacgaccggt agcacaagtc ctgccgtccc ttttcagatt acgttaagtg
     3781 aatgcagcaa agggacaacg ggggtgcggg ttgcatttaa cggtattgag gatgcagaaa
     3841 ataatactct gttgaaactg gatgaaggaa gcaatacggc ctccggtttg ggtatagaaa
     3901 tactggacgg aaatatgcgt ccggtgaaat tgaatgacct tcataccggg atgcagtgga
     3961 tcccattggt accagaacag aacaatattt tgccttactc cgctcgtttg aagtcaactc
     4021 agaagtccgt caatccagga ctggtgagcg cttcggcaac ctttaccctt gaatttcaat
     4081 aatcagagtc agatatgaaa agacttcaca agaggttcct gttagctacg ttttgcacgt
     4141 tatttacagc aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg
     4201 ctaaaccatg cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacac
     4261 gcaatctgca acaacctggt tctgcatctg gctggcacaa tattactttg tcattaaccg
     4321 attgtccgat tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg
     4381 gttattacaa aaatgaaggt actgccgaaa atattcagat agagctaagg gatgaccagg
     4441 atgcgacgtt aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg
     4501 cacagtttcc acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggaacga
     4561 tcgaggcgct aatcaatgtg atctatatct ggcaataaca tcaatttgat tttatttgcc
     4621 tcctttttaa atcaattttt taaccttagg atatatcatg ggtaaaacat tcagtataaa
     4681 ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa gtttttgcct ttagttgtaa
     4741 tgtcgatggc gggtcaagta ttggagcagg tacaacctct gtttatgtta atctcgatcc
     4801 tgtaatacta ccgggccaga atctggttgt agacttgtct cagcatataa gttgctggaa
     4861 tgattacggc ggctggtacg acactgatca tataaacctg gtacaaggat cagctttcgc
     4921 aggctcccta cagtcatata aaggatcact gtactggaat aatgtgacct accccttccc
     4981 gttgacgaca aataccaacg tattggatat tggtgataag acaccgatgc cattaccgtt
     5041 aaaactctat attaccccag tcggagcagc cggtggagtt gtcataaaat caggggaagt
     5101 tattgcccgt attcatatgt ataaaattgc cacgttagga agcggaaatc cacgtaattt
     5161 tacgtggaat attatttcaa ataacagtgt tgttatgcca acaggcggtt gcaccgttga
     5221 tagtcgtaat gtcaccgtga atctcccgga ctttcctgga agcgcagaaa taccccttgg
     5281 tgtatattgc tctagcgaac aaaagctctc attttatctt tcgggtgcca ccactgacag
     5341 tgcccgacaa gtgtttgcta acacagcacc agatgcaaca aaagcatcgg gcgtgggtgt
     5401 ctctctgatg cgtaatggaa aaacactggc aacaggagaa aatgtctccc tggggacagt
     5461 aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag gaaataaggt
     5521 tgcagccgga gccgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt
     5581 ttgacattag tctatgcgct ccattcacga aaattgcgcg gataattatt ttgtgaaggc
     5641 tattatccta cacttgtaag aaaatccgcg cacaattgaa gccagagtct tattttttat
     5701 attgagaatt atgttaattt aaaccctatg agagatgatg cttaacttat cgtatttata
     5761 ct
//
LOCUS       DAPQZD010000235         5754 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPQZD010000235 DAPQZD010000000
VERSION     DAPQZD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.237"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            103..2514
                     /locus_tag="RO454_004628"
     CDS             103..2514
                     /locus_tag="RO454_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0936351.1"
                     /translation="MQLSDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIK
                     IDFFAQDNKLNARLTPQQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQK
                     LILNIPQSYLKPQDWLSTPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSS
                     LNLGGWRLRHNGNWSTNSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDS
                     FPFEGAQFSSDDGMIAPELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELR
                     DFNQIFTGDLEVEIREADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNS
                     VSEPQFVQSSAAIGLPYEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQ
                     FSQNYKSFGKQQGQSYRFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVD
                     EKNINSYHQRSRISTTISQDLQDWGQLYLSASKDQYWDTADGYNLSASYSLPFRYISA
                     MLSLGYNKSPYYHEADKSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGS
                     SQDGEFNYAVAQGWQNQNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGIT
                     LHPHGLTLSQPLSLDSASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQI
                     SLDVNSVKDNVELLNTDVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVV
                     TLDSENSINSSIVADQGQVYMSGLPEEDTLIAQWGEEASQKCKTNYKLQNKKAKFSEL
                     TLQCQ"
     gene            complement(2624..2896)
                     /locus_tag="RO454_004629"
     CDS             complement(2624..2896)
                     /locus_tag="RO454_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDY0936352.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKIREYDDIRS"
     gene            complement(2908..3138)
                     /locus_tag="RO454_004630"
     CDS             complement(2908..3138)
                     /locus_tag="RO454_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDY0936353.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            3319..4950
                     /gene="dcuS"
                     /locus_tag="RO454_004631"
     CDS             3319..4950
                     /gene="dcuS"
                     /locus_tag="RO454_004631"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDY0936354.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKAQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            4947..5666
                     /gene="dcuR"
                     /locus_tag="RO454_004632"
     CDS             4947..5666
                     /gene="dcuR"
                     /locus_tag="RO454_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDY0936355.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT         1583 a         1322 c         1305 g         1544 t
ORIGIN      
        1 ttattattgt taaggatcac catcatatgt ccggtattca gttgcgctgc cgtatctccc
       61 tcacctgaca atagtcatag gttctcattt aaccccgatt ttttgcaatt atctgatggt
      121 gataatgcca aaaatatcga tctcaattat tttgcaaacg caagcggtgc agctccaggg
      181 gaatatactg ttgatgtagt tatgaatggg gaattagttg accgtcagat taagatagat
      241 tttttcgcgc aggacaacaa gctgaacgcc agactgacgc ctcaacagtt ggaacgttgg
      301 ggaattgata caaataagct ctccgtatct tccccatcgg cttaccagca aaatattgca
      361 tatattattc agggcgcaac agagaaattc gatgcgaaca accaaaagct aatattgaat
      421 ataccacaaa gctacctcaa acctcaggat tggttaagta ctccgcccca tctctgggat
      481 gaaggtatgc cagcgctaat ggttaactat ctatttaacg gaattaagca aaataataat
      541 ggctacggta gccgtagtca gtttttatct ctggacagtt ctctgaacct tggtggatgg
      601 cgccttcgcc ataacggtaa ctggtcaaca aactcatata ataaagagag ccactggcaa
      661 cccgttagtg tttatctaca gcatgattat tctttcctgc agggagggca atttactgta
      721 gggcaaacct caacggatgg tgcgatattt gacagttttc cttttgaagg agcacagttt
      781 tcatctgatg atggcatgat tgccccagaa ctcagccaat atagtcctgt tgtccgtggc
      841 attgcttatt ctcaagcaca ggtatccgtc aagcaaaacg gagttgtcat ttatcaaaag
      901 aacgtcccac ctggaccgtt tgaattacgc gattttaacc aaatattcac cggtgacctg
      961 gaggtggaaa tccgtgaggc agatggcact attcgccatt ttactcaggc gacggccgtt
     1021 ttaccgattc tgcaacgtca ggggcgctta cgctataacc tggcattcgg gaaatatcgc
     1081 tcaagttcta ctttgaataa tagtgtgtct gagcctcagt ttgttcagtc ttcagcagca
     1141 atcggattac cttatgaata tacattttat ggtggtggta tcaaagcaga caactatgca
     1201 gcagttctgc ttggcctggg taaatactca gatttttttg gagcgttctc cctggatgtc
     1261 actcatgctc gctcacaatt ttcgcagaat tataagtctt tcggaaaaca gcaaggacag
     1321 tcttaccgct ttatgtattc gcgtggtttt ggtgagacga atacgacatt gaatattacc
     1381 ggctatcgtt atgccacacg cggttactac gattttgatg agttacaaca aattcagagt
     1441 ggattcgttg atgagaaaaa tataaacagt tatcatcaac gttcgcgtat atcaacaacg
     1501 ataagtcaag atcttcagga ttggggacaa ctctacctct cagcaagtaa agatcagtac
     1561 tgggataccg ctgatggata taacttatct gcgagttact cgctgccatt ccgctatatc
     1621 tcggcaatgc tatcgctcgg gtataataaa agcccgtatt accacgaggc tgataaatcg
     1681 ctgtttttat ctgtgtctgt accgctcaac tcattgctga atggaaataa cctgttctta
     1741 accacgaata ccattacgaa taatggtcag gtgcaacagc aggttggatt aaacggctcc
     1801 tcacaggatg gtgagtttaa ctatgccgtg gcgcaaggct ggcagaatca aaacagagga
     1861 gagtcaggta acattaacct taactatcgt ggaccttatg cacaaatgaa cggcggctat
     1921 gcctggcaaa aagaatccag tcaatggaca tacggaatta gcggtggtat tacgttgcat
     1981 ccacatggat taaccttatc ccagccatta agtctggaca gtgccagtgc tctggttcag
     2041 gctcgtgatg ccgccagcgt aaaagtattg aacgggtcag gtatttatac ggactggcgt
     2101 ggatatgccg tggtcccata tttgaatccc tataaccgaa atcaaatttc acttgatgtt
     2161 aatagtgtta aggataatgt tgagctgcta aatacagatg ttactgtcat cccttctcga
     2221 ggagctttag tttccgctcc atttaaagtc aatgtcggta ataaggcaat aattaccttg
     2281 atacagaaga atggtgagcc cgtaccgttt ggtagtgtgg tgacattaga ttctgaaaat
     2341 agcattaaca gcagtattgt tgctgatcaa ggacaggtgt atatgtcagg gttacctgaa
     2401 gaggatacac ttattgcaca atggggtgag gaggcttctc aaaagtgtaa aacaaattac
     2461 aaattgcaaa ataaaaaagc gaaattcagt gaattaactt tacaatgcca atagtcggta
     2521 gttcttagat taataaccct gcatatgcgg ggttattttc atctaacata ttaacaacat
     2581 cattccagat ttgtattact tccggcggac acaaatcatc ttttcaactg cgaatatcat
     2641 catactcccg tattttatca aattcgtgtt tcgcaaatgg gcatagtggg ataatttttc
     2701 gtttttcccg acgcattttt tccacgactt tcgcaaccag ctgtttaccg atcccttgtc
     2761 ctttcaggct ttcatcgaca tcggtatgtt cgataatcgc taaattctct ccggtcggca
     2821 caaagacaat ttcagcgatt tgcttgccct gttcgtcatt aatgtaaaat ttattgtggc
     2881 cttcgcgtat ttccatttta ccctcgctta tttatgacgg tatttcagcg cgccgcttgg
     2941 gcacgtatca atcactttca ccacagtggt gacgtcgact tcatccggca tgatccacgg
     3001 ctttcgtttg agattaaata acttgccgtt gccacgtacg caattgccag aatgctgaca
     3061 tatcgcagtg ttaaaataga catcgatatt ttcgccggta taacagcgat aacccccgtc
     3121 cagtagcgcc tgatccatga ccttccctct ctcatcagta tggttttcac tataagcata
     3181 gccccggaat cttcatacag cactggcagt ttccgttgcc agagtctctt cccctatata
     3241 gccaatcatt cttactccct ttgaattacc cgcctcatca gagataatgc ttaagaaatt
     3301 tgtcacacaa ggaagctgat gagacattca ttgccctacc gtatgttacg caaacgtccg
     3361 atgaaattga gtaccacagt gatcttaatg gtcagcgcgg tactgttctc ggtgctattg
     3421 gtggtgcatc tgatttactt ctcgcaaatc agtgatatga cgcgagacgg gctagctaac
     3481 aaggcactgg cagtagcgcg taccctcgcc gactcgccgg aaatccgtca gggcttgcag
     3541 aaaaaagcgc aggagagtgg aatccaggcc atcgcggaag ccgtgcgcaa acgcaacgat
     3601 ctgctgttta ttgtcgttac cgatatgcaa agtcttcgct actcgcatcc tgaagcccag
     3661 cgtattggtc agccatttaa aggtgatgac atccttaaag cgctgaatgg cgaagaaaat
     3721 gtcgctatca atcgcggttt tctggcgcag gctttacgcg tatttacccc catctacgat
     3781 gaaaatcata aacaaattgg cgtagtggcg atcggccttg agttaagccg cgtgacccaa
     3841 cagatcaatg acagtcgctg gagcattatc tggtcggtac tatttggcat gctggtcgga
     3901 ctgatcggca catgcattct ggttaaagta ctgaaaaaaa tccttttcgg cctggaaccc
     3961 tacgaaatct ccacactgtt tgagcaacgc caggccatgt tgcagtccat caaagaaggc
     4021 gtcgttgccg tggacgatcg cggcgaggtc accctgatca acgatgccgc acaagaattg
     4081 ctgaattacc gtaagtcgca ggacgatgag aaactgtcga cgctaagcca ctcgtggtca
     4141 caagtggtag atgtctcgga agtgttacgc gacggaaccc cacgccgcga cgaagagatt
     4201 acgattaaag accgactatt actgatcaac accgttccgg tgcgcagtaa tggcgttatc
     4261 atcggtgcca tttcaacctt cagggacaaa actgaagtac gtaaactgat gcagcgactc
     4321 gacggtctgg tcaactatgc tgacgcactt cgtgaacgat cccacgaatt tatgaataaa
     4381 ttgcatgtga ttctcggatt attgcatctg aagagttata agcagttgga agattacatt
     4441 ctcaaaacag ccaataacta tcaggaagag attggctctc tgctgggtaa gatcaaatct
     4501 ccggttatcg ctggtttttt aatcagcaag attaaccgcg cgaccgattt aggccatacg
     4561 ctgattttaa acagtgaaag ccagctgcca gacagcggta gtgaggatca ggtcgcgacg
     4621 ctgattacca cgttgggaaa tctgatagaa aacgcgctgg aggcattagg accggaaccc
     4681 ggtggcgaaa ttagcgtaac attgcactac cgtcacggct ggctgcactg cgaagttaac
     4741 gatgatggac cggggatcgc acccgacaaa atcgatcaca tttttgacaa aggtgtctcg
     4801 acaaaaggaa gcgagcgagg cgtcggttta gcacttgtca aacaacaggt agaaaatctc
     4861 ggcggcagca tcgccgtgga atcggaaccc gggattttca cacaattttt tgtccagata
     4921 ccctgggacg gggagaggtc gaacagatga tcaatgtatt aattatcgat gacgacgcaa
     4981 tggtcgcgga gctgaatcgc cgatacgtgg cacaaatccc gggctttcaa tgctgtggaa
     5041 cggcttcgac gctggagaaa gccaaagaga ttatcttcaa tagcgatacg cctatcgacc
     5101 tgatattgct agatatctat atgcaaaaag agaacgggct cgatttactg cctgtcctgc
     5161 ataacgcgcg ttgcaaaagt gatgtgattg tcatctcctc cgctgccgat gcagcaacca
     5221 tcaaagattc gctgcattac ggtgttgtgg attacctgat caaacccttc caggcttccc
     5281 gctttgaaga ggcgctcacc ggctggcggc aaaagaaaat ggcgctggaa aaacatcagt
     5341 attatgacca ggccgagctt gatcagctaa tccacggcag cagctccaac gagcaggatc
     5401 ctcgtcgctt gccaaaaggc ttaacgccgc agacgttacg cacgctgtgc cagtggattg
     5461 acgcgcatca ggactatgag ttttcaaccg acgagctggc taacgaggtt aacatctcgc
     5521 gtgtttcctg ccgtaaatac cttatctggc tggtcaactg ccacatcttg ttcaccagta
     5581 tccattatgg cgtcacgggg cgtccggttt accgttaccg tattcaggca gaacactact
     5641 cactactgaa acaatattgc caataacgga tcgctggtta tctgtaagta ataattatta
     5701 ccgtaatgca aaatacccct ggcacaagtt acatcgttca tcagtaaaac ttgt
//
LOCUS       DAPQZD010000236         5724 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPQZD010000236 DAPQZD010000000
VERSION     DAPQZD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.238"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..2641)
                     /gene="traG"
                     /locus_tag="RO454_004633"
     CDS             complement(<1..2641)
                     /gene="traG"
                     /locus_tag="RO454_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDY0936356.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWAAVFVLISLLVNIRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIYTQPDSFTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASNDPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYRYFYDSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATTTQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRLSGGMY
                     ADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIH"
     gene            complement(2638..4011)
                     /locus_tag="RO454_004634"
     CDS             complement(2638..4011)
                     /locus_tag="RO454_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDY0936357.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVHLISMTLPDINAGCGGIDAYLGSFSFINSEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNINDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(3998..4390)
                     /gene="trbF"
                     /locus_tag="RO454_004635"
     CDS             complement(3998..4390)
                     /gene="trbF"
                     /locus_tag="RO454_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDY0936358.1"
                     /translation="MTKNKKNQEFKIRKIRRNIEYSFRGSDRYFDLFIVFLVAGIVLW
                     AVMHVIFDVCIDSWMADPKLLNFQYMWNVLMKVIPFTLWALAAGFLVTFFLSPMCELI
                     FGNIMIFLLKRRMRRENTLREGKNDATH"
     gene            complement(4344..4717)
                     /gene="trbJ"
                     /locus_tag="RO454_004636"
                     /pseudo
     CDS             complement(4344..4717)
                     /gene="trbJ"
                     /locus_tag="RO454_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(4647..5201)
                     /gene="trbB"
                     /locus_tag="RO454_004637"
     CDS             complement(4647..5201)
                     /gene="trbB"
                     /locus_tag="RO454_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDY0936359.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWHPQGMAAQPAQPAA
                     DTPARTDKTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYG
                     FSVFPYTLDGQGDMAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGA
                     TDAAGFMARMDTVLQMYGGKKGAK"
     gene            complement(5188..5472)
                     /gene="traQ"
                     /locus_tag="RO454_004638"
     CDS             complement(5188..5472)
                     /gene="traQ"
                     /locus_tag="RO454_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDY0936360.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVHSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
BASE COUNT         1405 a         1527 c         1351 g         1441 t
ORIGIN      
        1 catgaatact attctgagta tctccgatat tccctttata ttctgtgacc atattatcaa
       61 cctgatgttt tacatcatta cggatattac tgtcctgcgt tctttgatca ataatggcct
      121 gatgcccctg atggtcagca ataatatcct ggctgccacc acccgaaggt acgctctcca
      181 ttcctttacc aatatcccca cgggattcac gccaggcatt atcaacacca ggctgcacct
      241 gttcctgaac aaaagaccat gccatcgccc gacggcgttc tgcgatctcc ggtgaactgg
      301 tgtttgtcag aatagcttcg gcatcctgcg gtgcgtgttt catcacatac tgtgcaaact
      361 gttgcgacag atcttcactc atctgcccac tcatgctttc agtacgggat gccatttcag
      421 catattcatg gctgcgggtc atattcgtcg tgtactgatc gtaactctgt ttcgctgaat
      481 tcagggctgc agacagctgg tccacacggg aatcagcatt attgtcagta tggctgccgg
      541 attcactgac tttgcgactg gtaaagtaat cgctggcctc tttaaagtct tgtgtagctc
      601 tggcatcgat atcatggcga gcatcatggg tagccctgct gccactactt gctgaatgtg
      661 agtcatcatc cgttgcatcc aagctcccct taaatccacc tctgatgcct accccaccac
      721 caacaccaag aattttaggt ttaacccccc attcagcatg agcatcagca tacattccac
      781 cagatagcct ggtacttgtt gatgccagtt cctgagtcgc ctgctcatta ctgatattat
      841 gcgccttcgc atagctttcc actgcactgc gcatacggct ggccatcatg gaatcctgtg
      901 cgctcatcgt gctgtcagca ccacctgtga cagaatcact gctcccccgg ttagaaccaa
      961 actgactgag cgagttccag gcactggcaa tactgctgct gaacccatgc agggcgcttt
     1021 ctgctctgtt cgatgcctcc cgggccattt cctgttgtgc cgctgcaatc tgacgcgttg
     1081 cattgatacc aaccggtaac cgggacatcg ctccgcttgc atccatcacc atattaccgt
     1141 cacgggtctg tgtggtggtt gcaccactgc cggtctggta catcatctga ccaaacgacg
     1201 tggtgctgtt ggtgctccag ctgaagccgt tcacgttttc cgtctgcatg ttgccgtagg
     1261 agtaattacc gtcaaccaca ctgcctgccg cactggcggt cgggctgata gcagaggaag
     1321 cgaaatggct gtacacactg gaaaatccgg ccccgagtcc cctgaccatc atccaggata
     1381 atggcgggat catcatggaa agatacccgg cagtggaagc cagatcggag tatttcagct
     1441 gtatctgtga aatttccgag agcaccaccg gtgcaccatt ctgcttcgca taaaatgtca
     1501 tggcgctgtt cagaatggcg tacaacattg gccatgactg caaccacatc agggcaaaca
     1561 catagccttt caggacagac agcgtcagtt tgttgaagac agccgccagt accaggagcg
     1621 ggaaaatacc gatggcgatc cccgtcagga tggtctgcgt catgggcagt gtccgcatcg
     1681 ccacatgacc gatggagaca tgtgccagac gttgcttctc catcgatgac gtggtggcca
     1741 gattcaccag gctggctgag tcaccattac gtgctgcata actggtgatc ccctccttca
     1801 gcgcattaat ggtgacattc tggcggatga tctggctggc tgatttactt gagtcataga
     1861 agtaacggta actgtcgcca atcagcgtac tgaacaacag gttcggatca gggcgaccac
     1921 caaataactg ctgggcataa taatgccagg tttttccgcc actctgtgtg tccagattca
     1981 gcttgtcttt cagcgaaacc gacgcgtcct tacaggtcac gaaattatta ttgctgtcat
     2041 aaacgccccg cagcggactg gggcgggaaa aaatcagcgt gtagggatca ttggaggcca
     2101 tcagctcctc cagcgtgtat ttgtggttca ggtaaatatc ccccatcaca cagttctgca
     2161 cataatcctg aaaaagattg gcgatttccg gattgcggga caaaaagtcg gtgctttttg
     2221 ataccagttc tgcgccaaac agcatccccg ttttgctgta ggtgaaactg tccggttgcg
     2281 tgtagatcat ctcgtaactg gccaccattg catgcccgat acgggtcgtc agtgaaagtg
     2341 gcatcgccag tccgaccggc acattatcca cccgatggac tttgaccagg tcactgttat
     2401 caataatctg caccgacgta cggatattaa ccagcaggct gataagtaca aacaccgccg
     2461 cccagcccag caaatccatc acgttatggc gctgtaccca cataaaggcc accgcaacaa
     2521 cagacagggt gagagcaatt ttttcaatgg aatcccacgt cctggttccc ataaaggcgg
     2581 caatggcgtt caggttattc cgcaaccatt caccaccggc aatcacataa acttcattca
     2641 cagcgtgctc cctccgaagt gatagttgtt ctggtaacga ctgagcatgc gggcggaaag
     2701 ctgctgacgc atgtagctca tctgacgatc gacgaccagc agcgcatcct gctgcacctg
     2761 tacctgtgac tgaaacgccg caatctgccg ggtggcatca ttaatattgt cgttaatatg
     2821 ttcgataact gcttcgtcat aatttcccgt ggccaccatc gcccgggcct gctgtatcag
     2881 ctcctgaatg tactgcagca ggatgtcgta accgatatag tccgtcagct ggtaaatcat
     2941 actgttggaa acaccgagca tctgcgggtc aaccaggtat ttgaagacgg ggatggtggt
     3001 actggaaata aagccttttt ccttgtcatc cagaggcgtg tcactgacag ccttgttctg
     3061 aatgctggcc agaagtttag taatctgaga cttcagtgca ttatcccggc tgatggtcac
     3121 cggtgtgtct gccaccactt tcaggcattt gtcagaatcg ttgcagtgat aaatttttgc
     3181 tgtaccgcct tccatcatgg cccggataat tgagcggtca gtggttctgg ccgatagcgg
     3241 tgtgatttcg ccgttgggac caaacaccag tgagccggtc agcgtcatca caaactcttt
     3301 cagctctttg ttgccgtcaa acattctgtt tttggaaagc gcattccaca tgatgttgat
     3361 gtttttggtc acccgctcct tatccttgtc gctggcttta tccctgacgc tgtcagattt
     3421 cccgccaacg gtacatccct gccgggaagc agcccagtca gcaaaaatat tgctctcacc
     3481 ggcaatgtcc tgacagactt tctgctggga cacctgcgtc cgggggaaaa gcccgccgat
     3541 aatcccctgt gccgcctggc aggaactgag gttcatactg ttaatgtcgc ttgccatttt
     3601 ctgcaggaag tcttttgcgg ttttgatttc cggcaccgtt gtctgcaggg caaggtcaaa
     3661 gaagtaaccg gcagcattgc tcataatctg cttaacaaaa cgctgcaact gctcgctatt
     3721 aataaagctg aacgagccga gataggcgtc gatgccgcca catccggcat tgatatccgg
     3781 cagcgtcatg gaaatcagat gaacattttt aacctgagta cgggcataca gggagccacc
     3841 gtaagcatag ccggcggcct gcccctgcca gacgcccggc tgggtggtat tggaggcgaa
     3901 gcccagcttg ttaaagaact gattcatgtc gctgttcaca tccgctgacg ctgcgggtgt
     3961 gaccgtcagt aaagcagcac aaagaaccag aagaggttta atgcgtggca tcattcttgc
     4021 cctcccttaa tgtgttctcc cggcgcatac ggcgtttcag caggaatatc atgatatttc
     4081 cgaatattaa ttcgcacatt ggggacagaa aaaatgtaac aagaaaccct gctgccagcg
     4141 cccataacgt aaacggaatg actttcatca gaacattcca catatactgg aaattaagta
     4201 atttcgggtc ggccatccac gaatcaatac agacatcgaa aataacgtgc ataacagccc
     4261 ataaaacaat tccggcaaca aggaaaacaa taaacaaatc aaaatatctg tcagaacctc
     4321 tgaaactata ctcaatattc cgtctaattt ttctgatttt gaattcctga ttctttttgt
     4381 tttttgtcat tataagtttc ctctgatatt actgatgatt cattcagacc ggtcatgcgg
     4441 ctgtaatata tttcagacac cgaaatccat cccacaattg ccccacaaaa aacacaacag
     4501 aaacagaaaa ggtaagacac agcgaaacga ctatttctac aggactggcg ggataaaaaa
     4561 gcccctgacg gataaaggga atattcaggc tgacgattat tcccgtcgcg atgccggata
     4621 ttccggcaat gagtaacacc acctgcttat ttcgcacctt ttttccctcc gtacatctgc
     4681 agaacggtat ccatccgcgc cataaaaccg gcggcatccg ttgccccctg taaaagcggt
     4741 aatgcctcaa gcgtgttgac gttgaccagg aaggtggtcg gtgtggccac cgggatattc
     4801 gggaagaagg tctgcatcac gtccggtggc accggtaatg cttccggaaa ggccatatcc
     4861 ccctgaccat ccagagtgta gggaaaaacg gaaaagccgt actgctgtgc cagctgcttc
     4921 agtaccgggt caaactggtg acagtaaggg caatgcccct gcataaacag caccaccttc
     4981 cagtcggcca ggttaacctg cctgccgttg ctgaggcgga accagcgggg agccggcttt
     5041 tctgtcttgt ctgtcctggc tggcgtgtct gctgccggtt gtgcaggctg cgcagccata
     5101 ccctgcggat gccagagtcg ttcaatttca tcccgggtgg aggcctgcac acctgtggcc
     5161 atcatcaaca gaataaccgg cagtaattta gtgagagaca tgtccgccct cctgctgctc
     5221 tgccatcatg gcctgttgcc tggcttccag tgcctcacgc tcttcccggc tgacacgggc
     5281 aatccatgca tccagcacct ggtatgcgcc gaacagcaca aggatggccg caatcagctc
     5341 cgccagaaaa aaagccatcc agggtttgct gtgaacaaga cgggcgacga tatgaaacca
     5401 gacgcccagg gaaaataccc acattcccgt aatatcaagc cggggtaaag agaatctgcg
     5461 tttacttatc atgttaaact ccggttatta tttacatgag agtattcatg tctgtaatat
     5521 tcgcgtttgt gtgatattag tcagaggtac aaatggaaaa gcgatctttt aaagaaaaac
     5581 tggaaatagt gagaaacatc ataagagagt cattatcagg taatgcggct tttgctttac
     5641 tggtgtattc attgttgcac actctcccca ttaaaacctt tgctgattta tttgtgacga
     5701 tactgttatt tatagcaatt atcg
//
LOCUS       DAPQZD010000237         5685 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPQZD010000237 DAPQZD010000000
VERSION     DAPQZD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.239"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="RO454_004639"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="RO454_004639"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDY0936361.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="RO454_004640"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="RO454_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDY0936362.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="RO454_004641"
     CDS             complement(1535..1801)
                     /locus_tag="RO454_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDY0936363.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="RO454_004642"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="RO454_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDY0936364.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1412 a         1470 c         1477 g         1326 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct caaagtagtc
      301 cgtgttccag cctatgatcc agaggccgta gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg tgatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cggcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcttgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcattccagg ggagcttatt cagataatcc ccaaagacct ttcatcctct attcttaaaa
     1081 tagtcctgaa tcagaaacag taattgagaa ccacaatgaa gaaagtagcc gcgcttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtagtgacaa aattgctgta acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa agatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac cgccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcacgact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tcttttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga acgattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcatg ggccggaaac ggcttgaaaa acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 tgctttgcaa actggtgccc tctcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccaacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgttgcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg ttatggcagt
     2461 gcgccaaacg ggttgcgcga tgttgtgtgc agcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacattgcga cgctgaaaag tcgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcaca tctcacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga
     2641 actgatgccg caggcagaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac aacgcggtct atgaccatgt tgaacaggcg atgaatgatt tcgctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcaccag tgaagaagtg gttgatgaat tgctgacccg
     2941 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtgcgcaa cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtgatgact gcactggcag aagcctttaa
     3121 taatggcgag cgcgaaaccc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtcactgc cgttgccgga
     3301 aaacacccta ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcgga aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcagc tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcagatt ctgcctggcg atagtgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcacg
     3901 tgaatccgta gaagaagttc cactgcagcc agttaatccg catagcgcca atcgaccgcc
     3961 ggtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg
     4021 gcttggcgac gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cacgcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgtg tcgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 accgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgatcgta gcaaactgga acgtattgat attcgtacat cgcagctaat
     4501 cacgccgctg tttgaatatt ccggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aatgccactg gctgttcttc
     4621 aatatacggc ggtaacctgc cttcaacacc atataccacc gatgccaacg gtcgtggacc
     4681 ggcatgggcg aactcgctgt ttgaagataa cgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctcgat caatttgccg ataaaatccc
     4801 gacggaatta ctgactgcgt tgaaatcaga cgccacgcca gaggttcgtc gtgaacaggt
     4861 agcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggc gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggagg tcaggcatcg aaagcgacac cgctgggtgc
     5101 agtaactaag tttggcgagc atggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca tcgcttatag
     5281 cccgtgtgaa gagcatggtt acgatctggc actcagccac gatcagatgc gccaactcac
     5341 tgctaccggc ttctggccgt tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agaagcactt gaagaaacgt tacttcacga
     5461 gcagcgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattcaaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc ttcttatgtg ttggaaaatc agtag
//
LOCUS       DAPQZD010000238         5668 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPQZD010000238 DAPQZD010000000
VERSION     DAPQZD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.240"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..127
                     /locus_tag="RO454_004643"
     CDS             <1..127
                     /locus_tag="RO454_004643"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDY0936365.1"
                     /translation="RQINVFLRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            199..1557
                     /gene="mltD"
                     /locus_tag="RO454_004644"
     CDS             199..1557
                     /gene="mltD"
                     /locus_tag="RO454_004644"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDY0936366.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1606..2376)
                     /locus_tag="RO454_004645"
     CDS             complement(1606..2376)
                     /locus_tag="RO454_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0936367.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2454..3254)
                     /locus_tag="RO454_004646"
     CDS             complement(2454..3254)
                     /locus_tag="RO454_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDY0936368.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3495..4409
                     /locus_tag="RO454_004647"
     CDS             3495..4409
                     /locus_tag="RO454_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0936369.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4406..5209)
                     /gene="dkgB"
                     /locus_tag="RO454_004648"
     CDS             complement(4406..5209)
                     /gene="dkgB"
                     /locus_tag="RO454_004648"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDY0936370.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5372..5448)
                     /locus_tag="RO454_004649"
     tRNA            complement(5372..5448)
                     /locus_tag="RO454_004649"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5412..5414),aa:Asp,seq:gtc)
     gene            complement(5503..5618)
                     /gene="rrf"
                     /locus_tag="RO454_004650"
     rRNA            complement(5503..5618)
                     /gene="rrf"
                     /locus_tag="RO454_004650"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1484 a         1371 c         1402 g         1411 t
ORIGIN      
        1 gcggcaaatt aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga
       61 agaaacatta ttgcaacaac ctgaagaacg ttttgcatgg ttaaggtcaa agaaagatag
      121 gttctgataa aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa
      181 gcaactattg acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg
      241 ggttgccaga gcaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa
      301 ggggaagcag caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg
      361 ccagatggtg acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaac
      421 gaccggattc gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta
      481 actttacggg cagagccgta tatgtactgg atagccgggc aagttaaaaa acgtaacatg
      541 cctatggaac tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct
      601 ggcgccaatg ccgcgggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg
      661 aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg
      721 aacatgatgc agcgtctgaa caagatgttt gacggcgact ggcttctgac cgtagcggct
      781 tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa
      841 tccacggact tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg
      901 ctggcattga gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc
      961 gatgaaagcc gtgctctggc acgtgtgcac ctgagcagcc cggttgaaat ggcgaaggtt
     1021 gcagatatgg cggggatttc cgtcagcaag ctgaagacat tcaacgcagg cgtgaaaggc
     1081 tccacgctgg gcgcaagtgg tccgcagtac gtgatggtgc caaagaagca tgcagatcaa
     1141 ctgcgtgaat ctctggcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac
     1201 aatacgccac ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagtatc
     1261 gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct
     1321 aagctgaagc caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcgactggca
     1381 aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct
     1441 aaacgccacg gcgtgaacat caaagatgta atgcgctgga acagcgacac tgcgaatctg
     1501 caaccaggcg ataagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa
     1561 accagataat aaaaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg
     1621 tgcttctagc atgatgatat cactggtgaa agagccatca ttctgcaagg caaaatacgt
     1681 tttcacctct gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg
     1741 cgtacgcatt ctcgctaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt
     1801 atcaactatc agattggctt cactgattaa cgtcaaccac tcaccgctgg cgtggttccg
     1861 tatgtgagat gtatcgcgta atgcttctac cgtctgtaac cagatgtcgc gcactgggtg
     1921 acccggagac attacgtcca tcacaatcag cagaccaccg ggtttcaata cccggttcac
     1981 ttcacgcaat gctgcaccaa catcatgcca atgatgggca gaataacggc tgataacaat
     2041 atcaaatgcg ttatcggcaa atggcaggct ttcgacataa ccctggcggg tggtgatatt
     2101 tttcagttgc cgggcttcgg cagcttgtgc cacgacatcc agcatatggg cagataagtc
     2161 atacgccacc accgtgctca cgttttgcgc agcgacaaag ctggcatgcc ctgctccgca
     2221 gcccatatca agtacacttg catcaggata atcagccaga cgcaccgcca gacgctgtaa
     2281 atcttgtccg gatgcatgta cggtactggt cagatattcg ctggcttgag agctaaactg
     2341 cttttctacg tggtcatggt gggattgtgt tgtcattgcg ctgtcctttt gttatttgag
     2401 tataaaaggg cagcgccaaa cgttggtctg ccctgtggca gacctgacat accttattta
     2461 tcaggcttgc cgggactgaa ttcaacgagt agcggattgt gatcggacgc gcgcgtaacc
     2521 agtacagaag cttcgctgac gttcagacca cggtagaaaa caaaatcgag cgggcgacca
     2581 aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca
     2641 aagcgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
     2701 accgggccac tgtggtgagc tatctgatcg ccaataggaa gtaactgctt actatagaca
     2761 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca
     2821 ggtaacggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
     2881 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
     2941 tgtggcagca cgaaagcggg tacctgatcg gcggcaagat agttagcagt cgcaaactgt
     3001 actaactctg gcgttgtctg cgcttcctgc aataacacca gatgtgcatc tttgccgtag
     3061 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
     3121 cgaatacgct cttcggtact taacggttct ccaggtggta atgcctggcc gatgctcgca
     3181 aaagaccccg gcggtaagat cctttccgca ggttgtccgg caacatagcg catggcatag
     3241 gtgttttttc gcacttttga ctttgaaacc tctatcacgt caacccttcc gcactcggga
     3301 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
     3361 gtaagcaagt gcttacaccg gtcaggattt tgctcattat tgagaacatt aaatcggatg
     3421 gcatatctcg catcctgatg gtttaatgac aaataaattc aaaatatctc ttctattttg
     3481 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcagtcgtcg
     3541 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
     3601 gggcggtgaa aaagctggag atgaaacttg gcgttagcct gcttaatcgg accacgcggc
     3661 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
     3721 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttac
     3781 ggatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttgatt aagccttttc
     3841 gtgaacgcta tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga
     3901 tcgaaagaaa agtggatgtc gcgatacgcg ccggtacgtt aacggattcc agcttacgtg
     3961 ccaggccgct atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
     4021 acgggaagcc agaaacgatc gacgatttaa agcaacatat ttgcctggga ttcactgaac
     4081 ccgcttccct caatacctgg ccgatagcct gtagcgatgg acaattacat gaggttaagt
     4141 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
     4201 ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt
     4261 taatagcaga taaagtgttg ccagtagaaa tgccattcag tgcagtctat tacagcgacc
     4321 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
     4381 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa
     4441 ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta
     4501 aattttgtgc cttaagatta ctctccaggt tttcacgttt agtagaagaa ggaattactg
     4561 agtaaccttc ccccatagcc cacgccagaa tcacttgagc cggagtcgca ttgtgtttag
     4621 ctgcaatacg agcaataacc tcatctttca gtgccttgcc atacgccagc gtcatatagg
     4681 aagtaatatg gatactgtgc tgtttagccc aagcgaccac tttacggttt tgcagataag
     4741 gagagagttc aatctggtta gtagcgatgt tttcagcgcc aacagcagca atcgcttttt
     4801 ccatcaatgg gatcgtaaag ttggaaatac cgatctcacg cgtcagccct tgttttttgg
     4861 cttccagcag cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt
     4921 ggattagcgt cagatcaaca taatcggtac gcaatttttg caggctctct ttcagactcg
     4981 ggatcaattt gtctttgctg agattttcaa tccagatttt agtggtgatg tggagttcat
     5041 gacgtggcac gccactttct gcaatcgcct gacctactgc ggcttcgtta tcatagattt
     5101 gtgcggtatc aattgcgcga taatcaagtt caagcgccgt tttcacagat gaaataacaa
     5161 cgtcgtcttt cagacggaaa gtgcctaaac caaatgcagg gatagccata atatgcctct
     5221 tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc agactgttgc ggaaaaatgg
     5281 cgtaaaacgc agaggatttt tgcgattttg gcaataatag aaatacaaac gaaaaaaaac
     5341 ctcgagcgtt aactcgaggc ttcttagtaa gtggcggaac ggacgggact cgaacccgcg
     5401 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
     5461 tacaaccacc ggttttatga ccggcttact gcttaatttg atgcctggca gttccctact
     5521 ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc
     5581 attgggtcag gtgggaccac cgcgctaagg ccgccaggca aattctgttt tatcaacacg
     5641 tcccgttatg acctgttgat tcaatctg
//
LOCUS       DAPQZD010000239         5628 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPQZD010000239 DAPQZD010000000
VERSION     DAPQZD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.241"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            104..445
                     /locus_tag="RO454_004651"
     CDS             104..445
                     /locus_tag="RO454_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936371.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            491..928
                     /locus_tag="RO454_004652"
     CDS             491..928
                     /locus_tag="RO454_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDY0936372.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(970..1965)
                     /gene="wecH"
                     /locus_tag="RO454_004653"
     CDS             complement(970..1965)
                     /gene="wecH"
                     /locus_tag="RO454_004653"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDY0936373.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2140..2439
                     /locus_tag="RO454_004654"
     CDS             2140..2439
                     /locus_tag="RO454_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDY0936374.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2534..3445
                     /gene="glyQ"
                     /locus_tag="RO454_004655"
     CDS             2534..3445
                     /gene="glyQ"
                     /locus_tag="RO454_004655"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDY0936375.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3455..5524
                     /gene="glyS"
                     /locus_tag="RO454_004656"
     CDS             3455..5524
                     /gene="glyS"
                     /locus_tag="RO454_004656"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDY0936376.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1434 a         1359 c         1436 g         1399 t
ORIGIN      
        1 atttagtatg aatttattct gaaaatcatt tgttaatggc atttttcagt tttgtctttc
       61 gttggttact cgtaatgtat cgctggtaga tatggagatc gttatgaaaa cctcaaagac
      121 tgtggcaaaa ctattatttg ttgtcggggc gctggtttat ctggttgggc tatggatctc
      181 atgcccattg ttaagtggaa aaggctattt tcttggcgtg ttaatgacgg caacttttgg
      241 caactatgca tatcttcgcg cagaaaaact tgggcaactg gatgattttt ttatccacgt
      301 ctgtcagttg gtagcgttaa tcactatcgg tctcttgttt atcggtgttt taaacgcacc
      361 tatcaatgct tatgaaatgg tgatctatcc catcgccttt tttgtctgct tgtttggtca
      421 aatgcgtttg tttcgctcgg catgagcaac ataaagttct tacacattca ggaatgaaag
      481 gaatactgtg atggacaaca aaatatcaac ctattctccg gcatttagta ttgtttcatg
      541 ggtagccctt attggcggta ttgttaccta tctgttagga ctatggaatg cagagatgca
      601 gttaaatgaa aaagggtatt attttgccgt actggtatta ggtttgtttt ccgcggcgtc
      661 ttatcaaaag accgttcggg acaagtatga aggcatacct accaccccca tttattatat
      721 gacttgcctg acggtcttta ttatctctgt tgcgttgttg ctggtaggtc tatggaatgc
      781 aacgttatta ctcagcgaaa aaggctttta tgggctggct ttcttcttaa gcttgtttgg
      841 tgcagtagcg gtgcagaaga atattcgtga tgccggaata aacccaccaa aagaaacaca
      901 ggttacccag gaagaataca gcgaataatt cacgtaagcc cggtcagtcc aatgtgaccg
      961 ggcttctact taactcacta atctgtttct gtcgattcgt tgtaccagca tagaaagtaa
     1021 taaactcact gccagcgtcg cacaaaagat ccaaataata tccagtattg gccagttttt
     1081 aagctcaatc ccccgggtgc gcagtgcatg gataatcaat gcatggaacc cgtatatacc
     1141 taacgaatgg cgggagatta agccaagtcc gcaaatggta cgcgtataca gcgtgttttt
     1201 aaccagagtc aataacgcga ttgcgcagat aaaaaccatc ggcccacagt aaagatacca
     1261 ggtatcggca aaatttccgc gccactgtaa ttcatataat gtcccacgag agataataaa
     1321 aacccccgtc gcaaacagtg cggcgctcac ccacgacagt gctttatgct gtgtgtccat
     1381 catccctata gcgcggccca acatgccata aaggatgtag taaaaagtat cgccattgat
     1441 atataagtta attggcagcc attcaaaacc gtcgattttc tgcggcacgg tgtttgggtt
     1501 agcgatgata ccaatcacca ccattagcac cagcaacatt tttccgccga cgttcttcac
     1561 ctgaatcagc ggtgaaacca gataaatcac tgcaatcgcg aagaaaaacc ataaatggta
     1621 aaacactggc ttttgcagca ggtttttcag cgctaactcc acattgatgg aggtaaacag
     1681 cgcaatgtag agcagcgcga ttgcgctata aaaaaacagg cataagccga tacgcaagaa
     1741 atgacgcgac tgggcgctgc gttcgccaaa aaagagatag ccggaaatca tgaaaaatag
     1801 cggcacgctg acacgagagg cagaattcag aacattggcg atatcccagg tgatggggct
     1861 aacactatga gcattgatca cataccaggt agtggtgtga atcatcacca ccattaaaca
     1921 cgctatccct cgcaggttat caatccagta aattttgggc tgcatctgtg tctctgtatc
     1981 tggttaaaaa aagtctgacc gataaatcat tcagttggcg cactggaata atctgagttt
     2041 tatcactaca gcttatagag gcttaaggaa attcgtaaga tatcagccac tataccgata
     2101 taaataataa gactcacctg caaaccagac ggtaatttaa tgatgatgaa cgctttcttt
     2161 ccggcaatgg cgcttattgt gctagtgggt tgttctacac cgccacccgt gcagaaagca
     2221 caacgggtaa aggttgatcc tctgcgttcg ttgaatatgg aagcgttatg caaggatcag
     2281 gcggcaaaac gttataacac cggcgagcaa aaaatcgacg tcaccgcctt cgaacagttc
     2341 cagggaagct atgaaatgcg cggttatacc ttccggaaag agcagtttgt ctgttctttt
     2401 gacgcggatg gccatttttt gcatctttcc atgcgttaag ccctgctttt tcccgtttcg
     2461 tactgtatat cttccatcca gcgggtatac tgatcccttc ctttaaatcc acacgtatcc
     2521 agcacgaaat aatatgcaaa agtttgatac caggaccttc cagggcttga tcctgacctt
     2581 acaggattac tgggctcgcc agggctgcac cattgttcaa ccattggaca tggaagtcgg
     2641 cgcgggaacc tctcacccaa tgacctgtct gcgcgcgctg gggccagaac cgatggcggc
     2701 tgcttatgtt cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg
     2761 tttacagcac tactatcagt tccaggtggt cattaagcca tcgccggaca atattcagga
     2821 gctgtacctc ggttctctga aagagctggg catggacccg accatccacg acatccgttt
     2881 cgtggaagat aactgggaaa acccgacgct gggtgcctgg gggctgggct gggaagtgtg
     2941 gctaaacggc atggaagtga cgcagttcac ttacttccag caggttggtg gtctggaatg
     3001 taaaccggtt accggtgaga tcacctacgg tctggaacgt ctggccatgt acattcaggg
     3061 cgtagacagc gtttacgacc tggtctggag cgacggcccg ctgggtaaaa ccacctacgg
     3121 cgacgtgttc catcagaacg aagtggagca gtccacttac aacttcgaat acgcggatgt
     3181 ggacttcctg ttcacctgct tcgagcagta cgagaaagaa gcgcagcaac tgctggcgct
     3241 ggaaaatccg ctgccgctgc cagcctacga gcgtattctg aaagccgccc acagcttcaa
     3301 cctgctggat gcgcgtaaag ccatctccgt caccgagcgt cagcgctata ttctgcgcat
     3361 tcgcaccctg accaaagcag tggcagaagc atactacgct tcccgtgaag ccctcggctt
     3421 cccgatgtgc aacaaagata agtaagaggc ggctatgtct gagaaaactt ttctggtgga
     3481 aatcggcact gaagagctgc caccaaaagc actacgcagc ctggctgagt cctttgctgc
     3541 gaactttact gcggagctgg ataacgctgg cctcgcacac ggcaccgttc aatggtttgc
     3601 tgctccgcgt cgtctggcgc tgaaagtcgc taacctggcg gaggcgcaac cggatcgtga
     3661 aatcgaaaaa cgtggtccgg caattgctca ggcgttcgac gctgaaggca aaccgagtaa
     3721 agcggcagaa ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg agcgtctgac
     3781 taccgataaa ggcgaatggc tgctgtatcg cgcccatgtg aagggcgaaa gcaccgaagc
     3841 actgctgccg aatatggttg cgacttcgct ggcgaaactg ccgatcccga aactgatgcg
     3901 ttggggcgca agcgacgtgc actttgtgcg tccggttcac accgtgaccc tgctgctggg
     3961 cgacaaagtt attccggcta ccattctggg tattcagtcc gatcgcgtga ttcgcggcca
     4021 ccgctttatg ggcgagccgg aattcactat cgacaatgcc gatcagtatc cagaaattct
     4081 gcgtgagcgc gggaaagtca tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga
     4141 tgctgaagaa gcagcacgta agattggcgg taacgctgat ttaagcgaaa gcctgctgga
     4201 agaagtggct tcgctggtgg agtggccggt cgttctgacc gcgaaattcg aagagaaatt
     4261 cctcgcggtg ccgtctgaag cactggttta caccatgaaa ggtgaccaga agtacttccc
     4321 ggtgtatgcg aacgacggca aactgctgcc gaactttatc ttcgttgcca acatcgaatc
     4381 gaaagatccg cagcagatta tctccggtaa cgagaaagtc gttcgtccac gtttggcgga
     4441 tgccgagttc ttcttcaata ccgaccgtaa aaaacgtctt gaagataacc tgccgcgcct
     4501 gcaaaccgtg ttgttccagc aacagttggg gacgctgcgc gacaaaactg accgcattca
     4561 ggcgctggcc ggctggattg ctgaacagat tggcgctgac gttaaccacg ctacccgtgc
     4621 gggcctgctg tctaagtgtg acctgatgac caacatggtc ttcgagttca ccgacaccca
     4681 gggcgttatg gggatgcact acgcgcgtca cgatggcgaa gcggaagacg tagctgtcgc
     4741 gctgaatgag cagtatcagc cgcgctttgc cggtgatgac ctgccgtcta acccggtagc
     4801 ctgtgcgctg gcgattgctg acaagatgga taccctggcg ggtatcttcg gtatcggcca
     4861 gcatccgaaa ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg gcgtgctgcg
     4921 tattatcgtt gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag aagcagtgcg
     4981 tctgtatggc gataagctga ctaatgccaa cgtggttgat gatgttatcg actttatgct
     5041 cggtcgcttc cgcgcctggt atcaggacga aggttacacc gttgacacca tccaggcggt
     5101 actggcgcgt cgtccgactc gtccggctga tttcgatgcc cgtatgaaag cggtatcgca
     5161 cttccgtacc ctggaagcag ctgcagcact ggcggcggcg aacaagcgtg tatctaacat
     5221 cctggcgaaa tctgatgaag tgctgagcga tcgcgtgaat gcctctactc tgaaagagcc
     5281 ggaagaaatt aaactggcga tgcaggttgt ggtgctacgt gacaagctgg agccgtactt
     5341 tgcggaaggt cgttaccagg atgcgctggt cgaactggct gagctgcgtg aaccggtcga
     5401 tgctttcttc gataaagtga tggtcatggt tgatgataaa gaattgcgta tcaaccgtct
     5461 gaccatgctg gaaaaactgc gcgaactgtt cctgcgcgtt gcggatattt cgctgttgca
     5521 gtaataacgc cgttattaaa aagcctgcca tctggcaggc tttttttatc gctaaataat
     5581 acagcaacct ttaataatct tctgctgaat aaagattatc tcatatat
//
LOCUS       DAPQZD010000240         5473 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPQZD010000240 DAPQZD010000000
VERSION     DAPQZD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.242"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(102..1385)
                     /gene="aroA"
                     /locus_tag="RO454_004657"
     CDS             complement(102..1385)
                     /gene="aroA"
                     /locus_tag="RO454_004657"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDY0936377.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1456..2544)
                     /gene="serC"
                     /locus_tag="RO454_004658"
     CDS             complement(1456..2544)
                     /gene="serC"
                     /locus_tag="RO454_004658"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDY0936378.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2743..3435)
                     /locus_tag="RO454_004659"
     CDS             complement(2743..3435)
                     /locus_tag="RO454_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDY0936379.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3565..5325
                     /locus_tag="RO454_004660"
     CDS             3565..5325
                     /locus_tag="RO454_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDY0936380.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1424 a         1388 c         1275 g         1386 t
ORIGIN      
        1 accattaata aaaatacccc acaaggtccg aaaaaaaacg ccggcaatgt gccgacgtct
       61 ttgaaaagaa aggattgtct atgttatcgc ccgttattca ttcagccggg ttgacttatc
      121 cgcgccaatt gctcgaaata gtctggaaaa gttttagcgg tacattttgg atcaagaatc
      181 gtcacaggcg tatctgataa tgcgaccagc gagaaacaca tcgccatccg gtgatcatta
      241 taagtggcga tctcagcaaa tttcagtttt tccggtggcg taatgcgaat gaaatcatgc
      301 ccctcttcga cttccgcacc gactttacgc agttctgttg ccatcgcaaa caggcgatcg
      361 gtctctttaa cacgccagtt atagatattg cgcagcgtgg tggtgccttt tgcaaataac
      421 gccgcggtgg caatggtcat cgccgcatcg ggaatatggt tcatatccat atcaatggcg
      481 ttcagttcac cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt
      541 ttttccagaa catcagcaaa gcgaatatca ccctgcatac tgttgcgtcc aatgccggtc
      601 acttttacag tgccgcctct gattgctgct gctgccagga aataagaagc cgaagatgca
      661 tcgccttcaa ccaaataagt gcccggagac tggtaagact gccccccttt tacgacaaat
      721 tgttgatagt gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagggtgata
      781 tcgatataag gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca
      841 agaggcgcag tcattaacag tgcggtgagg aactggctgg aaacggagcc atcaacatca
      901 acgttgccgc cagtaaagcc gccctgtaaa cgcaatggcg gataattttc ttgttccagg
      961 taagtgatct tcgccccgcc ctggcgcagc gcatccacca gatgaccaat cgggcgttct
     1021 ttcattcgcg gttcaccgct cagtacaata tcattgctac ccaggcaaag tgctgccgcc
     1081 agcggacgca ttgccgttcc ggcgttaccg aggaacaact cccgggcact ttctgcgtgt
     1141 aatggaccac cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt
     1201 acccctaacg ctgttaatgc attcagcata tggcgcacgt catcgctatc cagcagattg
     1261 gttaatactg ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt agaaacgctc
     1321 ttggaaccgg gcagattaat agtgccatcg acacgagcga tgggttgtaa cgttagggat
     1381 tccatgaact caactctcta caacagaaat aaaaacccca caggctggct gtggggatta
     1441 tgcaaaattt cgacattagc cgtgacgacg ttcgaactca accatgaagt ctgtcagtgc
     1501 tttaacacct tccagcggca tggcgttata aatagacgca cgcattccgc cgaccactcg
     1561 gtgacctttc agcgcatgta agcccgcagc gaaagactct tcgaggaaca atttatcaag
     1621 cgcgctatcc gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacatc
     1681 attgcggtag aaatcgctgt tatcaatcac cccatacagc agttctgctt tttgctgatt
     1741 gatcttatcc attgcagcca caccgccgtt cgctttcagc catttgaaga ccaggccaga
     1801 taagtaccag gcaaatgtcg gcggcgtgtt gaacatggaa tcgttatcgt tgaggatgga
     1861 gtaatccaga atcgatgggc aggcgatatt cgctttgccc agcaaatctt cacgaacgat
     1921 gacgattgtc aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa ttacaccata
     1981 acggctgaca tcaatcggac gggaaagaat ggttgaagag aagtcagcgg cgaccaccac
     2041 gtctttaccg aagtctggtg tttcatcgat agcgatacca tcgatggttt cgttcgggca
     2101 gtaatgcata taagcagcat tatcagagag ttgccattca ctcattggct taactgcgcg
     2161 aagaccatca acggtcactt tcgcatcaaa cacatttggt gtgcagtatt ttttcgcttc
     2221 tttgatagca cttgctgccc agtaacccgc atcaacataa tctgcagtgg ttttatcacc
     2281 aagaatattc aacggtactg cagcaaattg accgcgacca ccgccatggc agaacaatac
     2341 tttgtagttg gaggggacgt taagaagatc gcgaaaatcc ttctcggctt cctctgcaac
     2401 ctgaatgaac tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca
     2461 gtcgcgcagt tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga
     2521 actaaaattg aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgcgg
     2581 tcaatcgatt gcgaccacag gtaatacatt attccagtgg ctatcggttt tatcattcag
     2641 tgacacacat cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc atcacacaaa
     2701 agaatctctc ggattattag caaaaagccg atgtttttgc tgtcaggtca cccgtttagc
     2761 tcgacttgcc ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc
     2821 gttcatatga atgatttcac tgcaacggat gcaggcatag tccgccgact ccggcaccac
     2881 tttgtaacgc tgagtacagt cactgggtaa aataagtaac cccggtttca ccttgtcatc
     2941 ttcaaagaaa taaacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc
     3001 caactgctct acaccgcgca atcgtagctc cataaagaac tcaaattccg tcatgttgga
     3061 gttattgagt ttcgaccagg ccagctcgcc gtcttcaata atgacaaccg gcttgccttc
     3121 cagaagatct tccagttttt cactgtgcgc catcaaccac attaccaggc ggtataacaa
     3181 cgccagagta ataaagacga taagtaccgg gaccatcggc acatcatcat aaaacgccac
     3241 atctcccgcc gctgaaccca gtgtcagaat gattaacact tcaaacagcg acatctgccg
     3301 cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg tataaagact
     3361 acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca tacggtggag
     3421 atcgaacgcc ttcattttta tgctcttatc gtggtttatc cgctaaaagc atagccaaca
     3481 gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt attgcgcgct
     3541 ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa agatgccgct
     3601 ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggttt tcagattgaa
     3661 gaggcctcct ggctgaatcc cgtgcctaat gtctggtctg tacatattcg cgacaaagag
     3721 tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaaa aagcggcgct ggcttctgcc
     3781 ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgcggactt ctggctgggc
     3841 gaaaccatcg ccaacggccc attcgtgcat tatcccaacg aaaaatggtt cccactgacc
     3901 gaaaatgacg atgtaccaga agggctgctc gatgaccgtc tgcgcgcatt ttacgatccg
     3961 gagaatgaac tgaccggtag tatgctgatt gacctgcaat ccggtaacga agatcgtggt
     4021 atttgcggtc taccgtttac gcgccagtcc gacaatcaga ccatttatat tccgatgaat
     4081 atcattggta acttgtacgt ttctaacggt atgtctgctg gcaatacccg taacgaagca
     4141 cgcgttcagg ggttatccga agttttcgaa cgttacgtga aaaaccgcat tattgctgaa
     4201 agcatcagct tgccggaaat cccggcagac gtgctggcac gttatccagc agtagttgaa
     4261 gcgatcgaaa cgctggaagc ggaaggtttc ccgatcttcg cttatgacgg ttcgcttggc
     4321 ggccagtatc cggtgatttg cgtggtactg ttcaatcctg ctaacggtac ctgctttgcc
     4381 tcattcggtg cgcatcctga ttttggcgtt gcactggaac gtaccgtgac cgagttgctg
     4441 caaggtcgtg gcctgaaaga tctggatgtg tttactccac caaccttcga tgatgaagaa
     4501 gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt aatctcctgg
     4561 gacctgttca agcaggatgc cgattatccg tttgttgact ggagcttctc cggcaccacg
     4621 gaagaagagt tcgccacgct gatggctatc ttcaaaaagg aagataaaga agtttatatt
     4681 gccgattacg agcatctggg tgtctactct tgccgtatta tcgtacctgg catgtccgat
     4741 atttatccgg ctgaagatct gtggttggcg aataacagta tgggcagcca tttacgtgaa
     4801 acgattctgt cactgcccgg tagcgagtgg gaaaaggaag attacctgaa cctcatcgag
     4861 caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgaactgtt gggcctggcg
     4921 accgggtcgg ataacggctg gtacactctg cgtatcggtg aattaaaagc catgctggcg
     4981 ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat ggagtttaac
     5041 tcatcagtat tcagcccgga acgcgccaat tattatcgct gcctgcaaac attgttatta
     5101 ctggcgcagg aagaagatcg ccagccgctg caatatctga atgcgtttgt tcgcatgtac
     5161 ggcgcagatg ccgtggaagc cgccagtgca gcaatgagcg gcgaagcggc gttttatggc
     5221 ttgcaaccag tagatagcga tctgcacgcg tttgctgcac atcagtcgtt gttgaaggcc
     5281 tacgaaaagc tgcaacgcgc caaagcagca ttctgggcaa aataaaatca aatagcctac
     5341 gcaatgtagg cttaatgatt cgtctgagtt atattacggg gcgttttttt aatgccccgc
     5401 tttacatata tttgcattaa taaaataatt gtaattataa ggttaaatat cggtaattcg
     5461 tatttaataa ata
//
LOCUS       DAPQZD010000241         5464 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPQZD010000241 DAPQZD010000000
VERSION     DAPQZD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.243"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            143..1357
                     /locus_tag="RO454_004661"
     CDS             143..1357
                     /locus_tag="RO454_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936381.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1414..2985
                     /locus_tag="RO454_004662"
                     /pseudo
     CDS             1414..2985
                     /locus_tag="RO454_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
     gene            3177..4718
                     /gene="istA"
                     /locus_tag="RO454_004663"
     CDS             3177..4718
                     /gene="istA"
                     /locus_tag="RO454_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDY0936382.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            4733..>5464
                     /gene="istB"
                     /locus_tag="RO454_004664"
     CDS             4733..>5464
                     /gene="istB"
                     /locus_tag="RO454_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDY0936383.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLT"
BASE COUNT         1317 a         1459 c         1458 g         1230 t
ORIGIN      
        1 gagcatactc atcagataat ctaccatcaa aagatggtta cataattttt gcattaaata
       61 aataaaatga ctctatacat aactttaaat ttcacttggt tattaacttc tggtcgataa
      121 tgcaaaataa agggatgtac tgatgccagt ccgacttaat accattcccg acgcagaacc
      181 ctatcccgta ccgccagccc gaattaagtg gctgacagct ttattactga tgctgtctgt
      241 cggtgtggcc ctgacttcat tattcgccag cgatgaactg gcaaaaaacg gcccgtattt
      301 ctgggggctg gcctgtggtg tgcctgcctt tatctggtca ctggttgctt cagttcgctg
      361 gctgtttttt atcacgcagt acattcggac tgatgcctgg aataagcgcc gggaagaggt
      421 gattttgcag gaaacgcgcc ggggacgacg ggcgttgcag atactcagtt tttccgtaca
      481 aaccgcgctg aatggtgatt ccgttgcaga aactataaca gcatttctgg cacgtcagca
      541 ggtgttgaat acctacgctg acttgcgcgg agaagatact gtgcgtcgca gcgtcatccc
      601 tgtattaacc aataaaccgg ttaccggacg tctatccgaa gttttttccc gtctgttcag
      661 cgatatcagg ctgcaactga cgcggctacc cgatgatttt catatcaacg tactactgga
      721 aattgatgcg ccgctttcag gggcctccgt gagaactatc tggcaggacg aatggcaaaa
      781 agccggactc ccggaagccc gtctgttttc agccccggag ccggggctgg ccgccgtgga
      841 cgactggctg gacaaccaca ttcaggaaaa ggccgtactg ctggtgattt ccgtgcgtct
      901 ggaaccgaag aacccggaac ggacggcaga atccgccacc gcgctgttgc tggcaaaccg
      961 gttgacgcaa accgcgctga cgccgctggc cctgctgcat cgtcctgaat gtatcactga
     1021 tacagagatg atggcgtcag gcattgcaca ggcgctggac tggatgccgg tgcagccaga
     1081 tgccatatcc ggtatgtgga ctgccgaact ggacagggaa cagcgtgccg cgttgttaag
     1141 cctgaaccag ccgtttgctc aggaagccct gatgtacgag ctggatgcct ttctggggcg
     1201 ctccggaccg gctgcgccgt ggctgtccgt cgctgtggca acgctggcgg caatacaatc
     1261 acaacacccc caactgacgc tcagcggcgt tcagggcggg cattattcat gggcgaccgt
     1321 ggtgtcgcca ttcgtttccc ctcaggaggc atcatagtgg ttccgggatt aaaccgtctc
     1381 cggccccgtt cggtttcagg gcctgccatc gtcatgaccg tggctgttgt ggttgcgctt
     1441 tgcacgttta ttcaccgtaa accggcattg ctgggcgttg aaccgggtag ctcacagcaa
     1501 atgtactgga tggctggcgt gattgccgca ggcgtggtct ttctgattat catgggggtt
     1561 tatcatctgc tgaaccgcca cgctgccgtg cagaactttg aactccgcag acagcttgcc
     1621 gccggagatg actatcgtgc ggtgacgaca aagaaagccg acagccgtca tccggcaatg
     1681 gtggaactcg ccgccttcct gcgcgaccgc tacggcccat tctggcggcg taaaatccgc
     1741 ctgctgctgg tgaccggcga gcctgaacag gcagaggcca tcgcgccggg gctgaccggg
     1801 caacactggc tggagggcga ccacacggtg ctgatatacg gcggcaggcc atcagcggag
     1861 cctgatgtcg cgctactgac cgccttaaaa aaactgcgcc gcagccgtcc gctggacggc
     1921 atcatctggc cgctgacaga agaacagagc cgccagacag cgcaactcga caaaggctgg
     1981 cgcgaactga taaacggcgg taagcgactc ggttttcagg ccccattcta tttgtggcag
     2041 gtctgtgaca acggtgatta tcagtccggg cgcgtcctgc aaagcgtcgg ctgcctgctg
     2101 ccggaaggct gtaccccgga acaactggct gcaatgctgg aagcacagac cctaccgctg
     2161 acggaacagg gcatgtcgca gctgctgacc gataaccgcc atgattttct gctgcgtctg
     2221 gcccataccc ttgcagagcg gggtatcacc cactggcaga ccgtcctgaa accgctgctg
     2281 gcaggcggcg cattttcttc cctgcgcctg cgcggcctga tgttcagccc gccgcttgcc
     2341 gccgtaccgg aggccgcacc tcatgcgtgg ctgccgtcgc cggtctgggc aggcatcacc
     2401 ggcgataacg cgcggggccg cgcggtgggc tttccgtggc tgcgtaccgc actgatgtcc
     2461 gctgtctgcg tgctggcgat agggggggcc ggaatgacga cctcgttctt cgccaaccgc
     2521 gcacttgttc aggaaaccag catccagacg gcgcgggcgc tcgatacccg cctgccgctg
     2581 gcagaacaac tggtggcgct gcataccctg cagggcgaac tggaacgcct gcaataccgt
     2641 atccgcgaag gtgcgccgtg gtatcagcgt tttggccctg aacgtaacca gcaactgctc
     2701 gccgccgcct ttcccggcta tgcgcaggcg gcaaaccggc tggtgcgcga cgtggccgtt
     2761 gaccatctgc aacagcaact gaacgccttt gtcgccctgc cgcccaacag tcctcagcgt
     2821 accgccaccg gtgaacaacg ctataagcag cttaaggcat tgctgatgac ttcccgcccg
     2881 gaaaaggccg acgctgcctt tttcagtacc acgctgatgg cagacggcct gcgctatgaa
     2941 aatatcccgg aaggcgtgcg gcagagcgtg ttgccgtcac tgctgcgtat tttcatgaaa
     3001 ggagatcact caataacttc catcgagatc gggtaataac atttgaacag atcgctgaat
     3061 aacatcgatg gagatcactt ttgactcatt ttgttattca gtgatctcca tcaatgttat
     3121 tggaacttca caggtgtgtt gatctgtatc ttttgccatt ccggtaaagg atacctatgc
     3181 caacagttcc aatttctatg agaaaactta aagaaattct taggcttaaa tacggtgttg
     3241 gactcagcca tcgacaaatt ggtcgtagtc ttgcaatctc cccttccgtt gtatccagat
     3301 atgctaatcg ggcggctcaa cttggcataa agcagtggcc cttacctaca ggatgggatg
     3361 atacaaaact aaaacatgcg ttccttcaga cccaggttaa gatgaagaag cactctctgc
     3421 ctgactgggc tacagtacac cgggaactgc gtaataaatg cgtgacgctg cagctactct
     3481 gggaagaata ctgtgagcgt aatccaggcg gtttttacag ctataaccat tactgccgga
     3541 tgtaccgtga atggctcaaa accacttcac catcaatgcg tcaggtacat aaagctggcg
     3601 aaaaactttt cgttgattac tgtggaccta ccgttggcgt taccgaccct gagaccggag
     3661 aaataagaac tgctcaggtc atcgtagctg ttctcggggc atcaagttac acatgggcag
     3721 aggccacctg gtctcagcag cttgaagact gggtgatgag tcatgttcgc tgcttccagt
     3781 ggttgggtgg cgttcctgaa cttgttgttc cggacaatct gaaaagcgcc acatccaggg
     3841 catgtaagta tgatcctgac gttaacccta cctaccagca gatgcttgag cattataatg
     3901 tcgcagtttt gcctgcgcgg ccacgtaaac cgaaagataa agccaaagct gaagttggcg
     3961 ttcaggttgt tgaacgctgg atcatggccc gaatcaggca tgagatcttc tacagccttg
     4021 catcgcttaa tcagcgcatt cgggagttgc tggaaagact gaataacaaa ataatgcaga
     4081 agttgggtta ttcacgtgca gaactcttca tccagcttga taaacccgca ctgaagcctc
     4141 ttcctgaagc cagttacagt tacaccctgg tgaagaaagt cagagttcat gccgattacc
     4201 acgtggaaat cgacaaacat tactactcgg ttccatgttc gctgttaggc cagcaactgg
     4261 aagcatggat ctccggagaa ctggtaagac tcttcaatca ggggcaggag gttgctgtgc
     4321 acccgcgcaa gcgtacttat ggctacagta cccgcaacga gcacatgcct gaagctcatc
     4381 gacagcatgc cacctggacg ccagagcgtc ttctggaatg ggcggggcac ataggcagtg
     4441 aaactcatag ttatgtgctt catatactga actctcgtcc acatccggaa caaagctatc
     4501 gcttctgcct tggactcctg aaccttcata aaaaatacag taaagccaga cttaatgcag
     4561 catgtgcaag agctctgaaa acaaaggtat ggcgtctgtc aggtattaaa tcgatcctgg
     4621 aaaaaggtct ggataaacaa cctgttcagg atccaaaacc agatctgtta tccacgatgg
     4681 aacacgaaaa cgtacgcggc agtgagtatt accactgata cgggatccaa tgatgaatca
     4741 tctttacgaa caactgaccg cacttaaact caccggcttc cgtgatgcgc ttaaaaagca
     4801 acttgctcaa ccgggcacat accaggagct gggcttcgaa gaacgcctgt cattactgac
     4861 agcagaagaa ctaacctgcc gtgaaaacag gaaggcagag cgtctgatca aacatgcacg
     4921 gttcagactt aatgctgagt tatcaaagct ggattatcgt aacaatagag ggctggacag
     4981 ggccctcatc cgttcactca gtcagggaaa ctggttaacc ctgaaacaaa atattttact
     5041 gaccggggcc accggcagcg gtaaaacgtt cctggcatgt gcacttggtc ataatgcctg
     5101 ccgacaggga tacaaggtct actattatcg ccttaaagcg ctgatggaac agtgctatca
     5161 ggggcatgct gatggaagat acagcaaact tttgaccagg ctgaataata gcgatctgct
     5221 gcttctggat gactgggggc tggaacctct ctcatcagaa cagcgtagcg acctgctgga
     5281 aatagtggat ctgatgtacc aacgaggctc aatcatcgta gtgagccagt tgccggtgga
     5341 aaactggtac aaaatgatcg gagactccac acatgcggat gccatcctag atcgactggt
     5401 tcatggcagt atcaagatcg aacttaaagg agaatcaatg cggaaaatac aatctccgtt
     5461 gacc
//
LOCUS       DAPQZD010000242         5461 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPQZD010000242 DAPQZD010000000
VERSION     DAPQZD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.244"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..562
                     /locus_tag="RO454_004665"
     CDS             <1..562
                     /locus_tag="RO454_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936384.1"
                     /translation="LSSKKQININKPGTDLTLFSFAGLSDVSGDYLDFSTRGENNQKV
                     FYRLRGVHYDGLVLLCGGQTYKYWNEANLKPVKTINGESPDENGNIDISLSPDPSPEI
                     GVVGAVGTYALVAINHAPQILPGSIWAGGDLYYASINNTGSYNTDIATKPFYDTPLIG
                     SWRAMAYSPGGVGDIGTACFLAIRIA"
     gene            562..951
                     /locus_tag="RO454_004666"
     CDS             562..951
                     /locus_tag="RO454_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936385.1"
                     /translation="MISVDSFRKITNPVFNDEHGLSINIICDIDGIGNDIPFTAMKND
                     CEEHGRVLYERALSGDYGSISKFVKKEQTQKLQTTLRTVGDLSIEAFSIQCAVDAGTA
                     SDAQIENLAELKEAIATRIGENREGIK"
     gene            948..2450
                     /locus_tag="RO454_004667"
     CDS             948..2450
                     /locus_tag="RO454_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDY0936386.1"
                     /translation="MSVTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSSYPTC
                     ASLLWGSELADNLVEFSTVMPGADGNNWIVEIVDLGINGNVATPGYSTLPDGSRKLTM
                     ITDGAMTPSKMYDQNQQYKEGLQIGEYINVTFGGDHAGTGTVFALPPTNLSGGKDESF
                     KYNVPTLIAGSQKKAGLLGGSGTLPPAVSEILNQEDAIIVVVRVEEDSDETKMRQNVV
                     NGINALLTSAQINQVTPRILIAPDYSANDYIAEQLEVVTNKLRGVGYIDSPRGATPAD
                     VVNRRQRYGGRMEILRPRVYSTSDVSGLSRPYSAIAAGLRARIDNEKGFWWSKSNQNI
                     YGITGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLSPQWSFECVR
                     RTADVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDEE
                     LNTKESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETV"
     gene            2468..2980
                     /locus_tag="RO454_004668"
     CDS             2468..2980
                     /locus_tag="RO454_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDY0936387.1"
                     /translation="MADSNTYRAFALFVQGERVLNCTEYTPVDMKIIEDDFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMGYLGLIPGAKTRFEIRSAYTDNYGVNFERIDTY
                     EGLITAITDDAQGTDSKSAVGQSVTIAPSYYKRVQNGKIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            2993..3274
                     /locus_tag="RO454_004669"
     CDS             2993..3274
                     /locus_tag="RO454_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDY0936388.1"
                     /translation="MESLLDSMTITLSRPFIIKGESRDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            3383..5023
                     /locus_tag="RO454_004670"
     CDS             3383..5023
                     /locus_tag="RO454_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936389.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLEKWTG
                     RAKGAAKWGGRAAAGAVRATGRGIKWGTLGAAGLIGGAAAGALAMNAETSEKLGLAKS
                     YGVGVEKYAAWENIGKAAGLNGENIGDLSEELTNKIGEIGNEKSLNPMLFQIGLTKKR
                     MAGWDREKQFNEVMRRISEMKDEKQAASLADQLMGGEANKIMTYMKATGKSWEQTMSD
                     AQKSNLLTKEGAEGAARAHVSVTNLWGSITSGLADTLGKIGGELAPTFDSVRETFTSW
                     FKDNQGGFVSTITEWVKPENMKKMWDGIVNFGEGCVKFGKIIWAVVKKLEWLIPDEKS
                     DEEQKTYNDEYNRAYQEFMDSGGKYSPNAGLTADNIAKAKAEEAVENMRHPERQEQAK
                     SQAESMLAFVNPLSGMVNKNTAPAALDSSTLNIDTLKQAVSTPAPEQNNKIEINIVGA
                     TDPQSIQQSAASGVLDGLRQAASSYNRGAMFDKPAAAG"
     gene            5059..5448
                     /locus_tag="RO454_004671"
     CDS             5059..5448
                     /locus_tag="RO454_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936390.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRFGKNDALQITGR
                     PNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVKGYWVLRELTET
                     QNSFLMGGTPKSQDFSLTLEYYGASLD"
BASE COUNT         1647 a         1069 c         1414 g         1331 t
ORIGIN      
        1 tttatcctct aaaaaacaaa taaatataaa caaaccgggc actgatttga cgttgttttc
       61 atttgctggt ttatcagatg tgtcgggcga ttatttagat ttttcgaccc gtggagaaaa
      121 caatcagaag gttttttata gattacgcgg agttcactat gatggcctgg tgttactgtg
      181 cggcgggcag acgtataaat actggaatga ggctaatctt aaaccggtta aaacgattaa
      241 cggggagtcg ccggatgaaa atggcaacat agatataagt ctttcaccgg acccatcacc
      301 ggaaataggt gttgttggcg cggttggtac ttatgcgctt gtcgctatta atcatgcacc
      361 acaaatactc ccgggctcca tatgggctgg aggggattta tattacgcta gtataaataa
      421 taccggttca tataacacgg atattgccac aaaaccattt tatgacacac cacttattgg
      481 ctcatggcga gccatggcgt attcacccgg tggagtcggt gatattggga ctgcctgttt
      541 tttagctatt aggattgcgt aatgataagc gttgattcat ttagaaaaat aacaaaccct
      601 gtttttaatg acgagcatgg gttatctata aatattattt gcgatattga cgggattgga
      661 aacgacattc cattcaccgc aatgaaaaat gattgcgagg aacacggtag ggttttatat
      721 gaaagagcgc tgagcgggga ttatgggagc atttctaaat ttgtaaaaaa agagcagacg
      781 caaaaactcc agactacatt acggactgtt ggcgatctgt caattgaggc attctcaatt
      841 cagtgtgcag tagatgccgg aaccgcatct gacgcgcaaa tcgaaaattt ggccgagctt
      901 aaagaagcaa tagcgacgcg aattggtgaa aatagagaag gaataaaatg agcgtaacta
      961 attttttaca cggtattcgc acgctcgaat acgacgacgg cacaaaagag atcaacacag
     1021 tagatatttc cgtgattggg attgttggta ctgctcccga ttccagctac ccaacttgtg
     1081 catcactatt atggggatca gagctcgcag acaatcttgt tgagttttcg actgtcatgc
     1141 caggggccga cggaaacaac tggattgttg aaattgttga tttgggaatt aacgggaatg
     1201 tagcaactcc aggatacagc acactgcccg acggttcgag aaaattaaca atgatcacgg
     1261 acggtgccat gaccccgtca aaaatgtacg atcagaacca gcagtataaa gaaggactcc
     1321 agattggaga atatattaac gtcacattcg gtggtgatca cgcaggaacg gggactgtat
     1381 tcgcgctccc cccaacaaat ttatccggtg gtaaagacga atcgttcaaa tataatgttc
     1441 cgacgttaat tgcaggcagt caaaaaaaag ctggattgct cggtgggtct ggaacccttc
     1501 ctcccgctgt atctgaaata ctaaatcagg aggatgccat tattgttgtt gtccgcgtcg
     1561 aagaggacag cgacgaaaca aaaatgcggc agaacgtcgt taacgggatt aacgcattgt
     1621 tgacgtctgc gcaaataaat caagtaacgc cgagaatttt aatcgctcca gattatagtg
     1681 ctaacgatta tattgcagaa caacttgaag tggtgacaaa taaattacga ggcgttggat
     1741 atatcgattc accgcgaggc gctacgcctg ctgacgttgt aaatcgtcgt cagaggtacg
     1801 gcggcagaat ggaaattctg cgccctcgtg tttattctac gagtgatgtt agtggtttat
     1861 ctcgtccgta ttcagctatc gctgctggtt tacgagccag aattgataac gaaaaagggt
     1921 tttggtggag caaatcgaat caaaatatct acggaattac aggactcgaa caagtcgatg
     1981 attttataat cggtgaaacg aactgcacag caaatctgct gaacgccagc caggtcagca
     2041 caattattcg ctatgatgga ttcagacact ggggtaacta tctgtgtagc ctcagtcccc
     2101 aatggtcctt tgaatgcgtt cgtcggacag ctgacgtaat tgaggattca atagcccggg
     2161 caatgatgac cgattttatt gatcgcccga tagacctgca cctcggaaca gacgttgtag
     2221 agtcgataaa cgcgtatctg cataaattag aggagcaggg cgcgattaac ggaggacggg
     2281 cgtggttaga tgaagagtta aatacaaaag aaagtctggc cgctggtaat ctttatatta
     2341 atgttgactt tggaccgaaa tccccggcac aaacaattac gttaatgtac cgcattaata
     2401 atgattacac agttgaagct ctggcctctc tttttaaaga aacagtctaa taatctggag
     2461 aattgatatg gcagatagta acacctatcg tgcatttgcg ttgttcgtcc agggagaacg
     2521 agttttaaat tgcactgaat acactcccgt cgatatgaaa ataatcgaag atgattttaa
     2581 gacgggcgct atggatacag ctatcactct ggacggggga atggagaaaa tgtcagccag
     2641 ttttaaagtc tctggctccg attctggtgt gatggggtat ttgggattaa taccaggtgc
     2701 taaaacaagg ttcgaaattc gaagtgcata tactgacaat tacggtgtca attttgaacg
     2761 tattgataca tatgaggggc ttattacggc gattactgat gacgcgcagg ggactgattc
     2821 aaaatcagca gttgggcaat cagtaacgat tgcgccgagc tattataaac gagttcaaaa
     2881 tggaaaaatt atttacgaga ttcatcctgc aaaaatgaaa cgagtaatta atggtgttga
     2941 cgttcttgcg ggcgttgctc gtattctcca tgtatattaa aaggcaaata aaatggaatc
     3001 attattagac agtatgacta ttacgctctc tcgcccgttt attattaagg gggagagccg
     3061 cgatacaatt actattcgcg aaccaaaatt acgcgaccgc attatgttca gtaacgataa
     3121 aagtggtctt gaagagcgga cagcgacaat gctggcgcgc ctggcaaatc tggagcgtga
     3181 ggatttatat gcactgcctg cgtgcgatta cgatcaaatg gaggccgcat ttaacgaact
     3241 ggtaaagcct ccgaaagacc gacatcagat atagttattc tgattccgtt tattgcgaaa
     3301 aaacttgcca tcccgcccga tacgcaaatg gacctgccgt accgggtttt taatttctat
     3361 gtaaacgagg tcatgaaaat cgatggctat ttctcaaaac tttaaaacgc aggttgtatt
     3421 cggtgggcga atagacccgt cgtttcgtcg cggtacaaca gaactcaatg atgcgattcg
     3481 gcagacgtcg tctactgtcg ggaaattaac gaaaagtcag gataaattaa aagacaaaat
     3541 cgcagcaatg aaactggcag gtaaagacgt ttctgattta tctgctcagt atcaaaagct
     3601 ggaccgtcga ataaaagcga caacgcagga ccaggaggcg ctgaatactc aacttgctaa
     3661 aaaacagcgg ctggagaagt ggacgggtcg cgcaaaagga gctgcaaaat ggggcggacg
     3721 agccgccgca ggtgcggtga gggctacggg gcggggtatc aaatggggga cacttggcgc
     3781 ggcagggctc attggcggtg ctgcagctgg tgcgctggca atgaatgcag agacgtcaga
     3841 aaaactcggt ctggcgaagt cctacggcgt tggcgtcgaa aaatacgctg cgtgggaaaa
     3901 tattggtaaa gccgcgggcc tgaacggcga aaatatcggg gatttgtctg aagaacttac
     3961 aaataaaatc ggcgaaatcg gaaacgaaaa aagcctgaat cccatgttat ttcaaattgg
     4021 cctgacgaaa aaacgaatgg ctggctggga tcgggaaaaa cagtttaatg aggttatgcg
     4081 tcgcatctct gaaatgaaag atgagaagca agcggcgagc ctcgctgacc agctcatggg
     4141 gggcgaggct aataagatca tgacgtatat gaaggcgacg ggtaaaagct gggaacagac
     4201 aatgtctgac gcgcaaaagt cgaatttgtt aacaaaagag ggtgcggaag gggcggcgcg
     4261 tgcgcatgta tcagtgacaa atctgtgggg ctctattacg tcgggacttg cagatacact
     4321 cggtaaaatc gggggggaac tcgcgccgac gtttgactca gtacgtgaaa catttacatc
     4381 ctggtttaag gataatcagg gtggttttgt cagcacaatt actgagtggg taaaaccaga
     4441 aaatatgaaa aaaatgtggg atggcatcgt caattttgga gaaggttgcg tcaaattcgg
     4501 gaagattata tgggctgtcg taaagaaact ggagtggctt attccggatg aaaaatcaga
     4561 tgaagaacag aaaacttata atgacgaata taatcgggca tatcaagagt ttatggattc
     4621 gggaggtaaa tattcaccga acgctggatt gactgctgat aatattgcga aggcaaaagc
     4681 cgaagaggct gtcgaaaata tgcggcatcc agaacggcaa gaacaggcaa aatctcaggc
     4741 agaatcaatg ctcgcgtttg ttaatccatt atccgggatg gtaaataaaa ataccgcgcc
     4801 tgcagcactg gattcgtcga cgttaaatat tgatacgttg aagcaggcag tctcgacacc
     4861 tgcgccggag caaaacaata aaatcgaaat caatattgtc ggtgcgacag atccgcagtc
     4921 aatacaacaa tctgcagcgt cgggggtgct cgatggactg agacaggctg cgagttcgta
     4981 caatcgtggc gcgatgtttg ataaaccagc tgcggcgggg tgacaatgag tgatgcaata
     5041 aacggtgctg aggatatcat gctcggcctg ggtgatttca tttttgctat atcaacagta
     5101 gcgtacaaca aactgcaacg tagtgacgcc tggcgctggg ctcagcaaac acgattcggg
     5161 aaaaacgacg cgttgcagat aaccggacga ccgaacccca caattactat cgatggaaag
     5221 ataaacgctt tatttcttga cgggtgcggc gtcggcctgt tgacagattt gcgagcgctc
     5281 gggaacacgg gagaacccca gcagctagtg ctggggaccg gggaggtgaa agggtactgg
     5341 gtgctgcgag aactaactga aacgcaaaac agctttttga tgggcggaac acctaaatcg
     5401 caggactttt cactcacgct cgaatattat ggagcttcgc tagattaata caaaatcaag
     5461 c
//
LOCUS       DAPQZD010000243         5426 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPQZD010000243 DAPQZD010000000
VERSION     DAPQZD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.245"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            87..1421
                     /gene="garP"
                     /locus_tag="RO454_004672"
     CDS             87..1421
                     /gene="garP"
                     /locus_tag="RO454_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDY0936391.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1437..2207
                     /gene="garL"
                     /locus_tag="RO454_004673"
     CDS             1437..2207
                     /gene="garL"
                     /locus_tag="RO454_004673"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDY0936392.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2237..3127
                     /gene="garR"
                     /locus_tag="RO454_004674"
     CDS             2237..3127
                     /gene="garR"
                     /locus_tag="RO454_004674"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDY0936393.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3224..4369
                     /gene="garK"
                     /locus_tag="RO454_004675"
     CDS             3224..4369
                     /gene="garK"
                     /locus_tag="RO454_004675"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDY0936394.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4402..4778
                     /gene="rnpB"
                     /locus_tag="RO454_004676"
     ncRNA           4402..4778
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RO454_004676"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5344..>5426)
                     /locus_tag="RO454_004677"
     CDS             complement(5344..>5426)
                     /locus_tag="RO454_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDY0936395.1"
                     /translation="DHLEQLINVNVFSSDRMSFCECNRFP"
BASE COUNT         1299 a         1275 c         1506 g         1346 t
ORIGIN      
        1 cattgtcgta acggcataaa taaaactcat aaataacacc ttccccataa taataaaaat
       61 cagcataagt acccgaggta aataaaatga ttctggacac cgttgacgta aaaaagaaag
      121 gcgtgcatac ccgctattta atattactga ttatttttat tgttaccgcc gttaactacg
      181 ccgatcgtgc aacgctgtct attgctggta ccgaagtggc aaaagagttg cagttaagtg
      241 cggtttcgat gggttacatc ttctctgctt ttggctgggc ctacttgctg atgcaaattc
      301 ccggcggctg gctgcttgat aagtttggct cgaagaaagt ttacacttac agcctctttt
      361 tctggtcgct gttcaccttc ctgcaaggct ttgtcgatat gtttccgctg gcctgggctg
      421 ggatctccat gttctttatg cgatttatgc tggggttctc ggaagcgcca tcattcccgg
      481 cgaacgcccg tattgtcgcc gcctggttcc cgacgaaaga acgtggtact gcctccgcca
      541 tctttaactc ggcgcaatat ttctcgttgg cgctcttttc gccgctgctt ggctggctga
      601 ctttcgcctg gggctgggag catgtcttta ccgttatggg ggtgattggt tttgtgttga
      661 cggcgctgtg gatcaagttg attcataacc cgacagatca cccacgtatg tctgcagaag
      721 agctgaagtt tatctcggaa aatggcgcgg tggtcgatat ggaccacaaa aagccgggta
      781 gtgcggcagc aagcggaccc aaactgcatt acatcaagca attgctctct aaccgcatga
      841 tgctgggcgt atttttcgga caatatttta tcaacaccat cacctggttc ttcctcacct
      901 ggttcccaat ttatctggtg caggaaaaag gcatgtcgat tctgaaagtg ggtctggtcg
      961 cctcgattcc ggcactgtgt ggctttgcgg gcggcgtgct gggaggtgtc ttctcggatt
     1021 atctgatcaa acgcggtttg tccctgaccc tggcacgtaa gctaccaatt gtgctgggaa
     1081 tgttgctggc ttccaccatc atcttatgta actacaccaa caacaccacg ctggtggtca
     1141 tgctgatggc gctggctttc tttggcaaag gatttggtgc gctgggctgg ccagtgattt
     1201 ctgacaccgc gccgaaagag attgttggcc tctgcggcgg cgtctttaac gtctttggca
     1261 atgttgcctc cattgtcact ccactggtga ttggctatct ggtaagtgaa ctgcactcct
     1321 tcaatgcagc actggttttc gtgggatgtt cagcgctgat ggcgatggtc tgctacctct
     1381 tcgtagtcgg cgacattaaa cgtatggaat tgcagaaata agcaaaggta taagcgatga
     1441 ataacgatgt tttcccgaat aaattcaaag ccgcactggc tgcgaaacag gtacaaattg
     1501 gttgctggtc agctctctct aacccgatta gcactgaagt tcttggtttg gctgggtttg
     1561 actggctggt gctggatggt gaacatgcgc caaacgatat ctccacgttt attccgcagt
     1621 taatggccct gaaaggcagc gccagcgcgc cagtagtgcg agtgccgacc aacgagccgg
     1681 taattattaa gcgtcttctg gatatcggtt tctataactt cctgattcct tttgtagaaa
     1741 caaaagagga agcagagcag gcggtggcat caactcgtta cccaccggaa ggcattcgcg
     1801 gcgtctccgt ttctcaccgc gccaatatgt ttggcaccgt ggcggattat ttcgctcagt
     1861 cgaacaagaa catcactatt ctggtccaga tagaaagtca gcagggcgta gataacgtcg
     1921 atgctattgc cgctaccgaa ggtgtagacg gcatcttcgt cggccccagc gatctggctg
     1981 cggcattagg ccatctcggc aatgcctcgc acccggatgt acaaaaagca attcagcaca
     2041 tttttaaccg tgccagcgcg cacggcaaac ccagcggcat cctcgcaccg gtcgaggctg
     2101 atgcgcgtcg ttatctggaa tggggcgcga cgtttgtggc cgtcggcagc gatctcggcg
     2161 tcttccgctc tgccactcag aaactggctg atacctttaa aaaataacca ccaccgcaac
     2221 aagagagatg attgatatga ctatgaaagt tggttttatt ggcctgggga ttatgggtaa
     2281 accaatgagt aaaaaccttc tgaaagcagg ttactcgctg gtggttgctg accgtaaccc
     2341 agaagctatt gctgacgtga ttgctgccgg tgcagaaaca gccgctacgg ctaaagcgat
     2401 cgctgaacag tgcgacgtca tcataaccat gctgccaaac tcccctcatg tgaaagaggt
     2461 ggcgctgggt gagaatggca ttattgaagg cgcgaagcca ggtacggtat tgatcgatat
     2521 gagttctatc gcaccgctgg caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat
     2581 tgatatgctg gatgctccgg tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc
     2641 agtgatggtg ggcggcgaca aggctatttt cgacaaatac tatgatttga tgaaagcgat
     2701 ggcgggttcc gtggtgcata ccggggaaat cggtgcaggt aacgtcacca aactggcaaa
     2761 tcaggtcatt gtggcgctga atattgccgc gatgtcagaa gcgttaacgc tggcaaccaa
     2821 agcgggcgtt aacccggatc tggtgtatca ggcaattcgc ggtgggctgg cgggcagtac
     2881 cgtgctggat gccaaagcgc cgatggtgat ggaccgcaac ttcaaaccgg gcttccgtat
     2941 tgatctgcat attaaggatc tggcgaatgc gctggatact tctcacggcg tcggcgcgca
     3001 actgccgctc actgctgcgg ttatggagat gatgcaggca ctgcgtgcag atggtttagg
     3061 aacggcggat catagcgccc tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac
     3121 tcgctaatga cgtcgtgccc ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa
     3181 taagagaaac gccctccggc gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc
     3241 cccagactct tataaagaaa gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg
     3301 atttcgggaa atttttcctg atgcacagta tgtttctgtt ccggttgccg acggtggtga
     3361 aggaacggtg gaagcgatga ttgcagccac ccagggggct gaacgtcacg cctgggttac
     3421 agggccgctg ggcgagaaag tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc
     3481 gtttatcgaa atggcggcgg ccagtgggct ggaactggta cctgcggaaa aacgcgatcc
     3541 actcgtgacc acttcacgcg gtactggtga gttaatcctg caggcgctgg agagcggtgc
     3601 gacaaacatt attatcggca ttggcggcag cgccaccaac gacggcggcg caggcatggt
     3661 acaggcgctg ggggcgaaat tatgcgatgc caacggcaat gaaattggtt ttggcggcgg
     3721 tagtcttaat actctgaatg atattgatat ttccggtctc gatccgcgct taaaagattg
     3781 tgtcatccgc gtcgcttgtg atgtcaccaa cccgttagtc ggcgacagtg gtgcttcgcg
     3841 catctttggc ccacaaaagg gagccagtga ggcgatgatt gtcgagctgg acaataacct
     3901 ctctcactat gccgatgtta ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg
     3961 cgcaggagct gcgggaggta tgggcgcggc gctgatggcg tttcttggtg cagaactgaa
     4021 aagtggtatt gaaatcgtca ctacggcgct gaatctggag gaacatattc acgattgtac
     4081 gctggtgatc actggtgaag ggcgtattga tagccagagt attcacggca aggtaccgat
     4141 tggtgtcgca aacgtggcga agaagtacca taaaccggtg attggcattg cgggtagcct
     4201 gaccaatgat gttggcgttg tacatcagca tggcattgat gcggtcttca gcgtactgac
     4261 cagcattggt acgttagacg aagcactccg cggagcttat gacaatatct gccgtgcttc
     4321 acgtaatatc gccgcgacac tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc
     4381 aaaccctcta tactgcgcgc cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct
     4441 tcgggggaga cgggcggagg ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa
     4501 cgcctggggg ggaaacccac gaccagtgca acagagagca aaccgccgat ggcccgcgta
     4561 agcgggatca ggtaagggtg aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt
     4621 ccgtggcacg gtaaactcca cccggagcaa ggccaaatag gggttcataa ggtacggccc
     4681 gtactgaacc cgggtaggct gcttgagcca gtgagcgatt gctggcctag atgaatgact
     4741 gtccacgaca gaacccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
     4801 ggcgggtttt tgctttgtgt gcccagggta agatgaatat ctacggtatt aataacagac
     4861 tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaattata cgtatatttc
     4921 tctcattaat tgtaagttcg caggtattgt ccgatcgttg aataatttgg gcgttataac
     4981 ctaaattttt accgtccaat aatcaatatt tctattgtga ttgttaacca ctgcgtgcat
     5041 tgacgggatt gaagggtgaa gaatatgtgc aattgagaag cctttcattg atgcctgcct
     5101 tgtcatcatg acaaacaata atagatgctt attctccttg cacggttctt tcgcactatc
     5161 aaaaaacgct catttacccg gcattacagt caacgaagca acattctatg gcctacgctg
     5221 atgtaaagca cagtgagaat cccccttgtt gtcttcgtct gaccttatcg cggcgaccag
     5281 caattattcg ttttgtgatc ctcaacacaa aatgctcttc gttgcctatg atgggagaag
     5341 aatttatgga aaacgattgc attcacaaaa tgacatcctg tcagaagaga aaacgttgac
     5401 gttaatcaat tgttcgagat gatcaa
//
LOCUS       DAPQZD010000244         5378 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPQZD010000244 DAPQZD010000000
VERSION     DAPQZD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.246"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..1250)
                     /locus_tag="RO454_004678"
     CDS             complement(<1..1250)
                     /locus_tag="RO454_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0936396.1"
                     /translation="MAADTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYA"
     gene            complement(1325..1993)
                     /locus_tag="RO454_004679"
     CDS             complement(1325..1993)
                     /locus_tag="RO454_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDY0936397.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            complement(2029..2265)
                     /gene="merE"
                     /locus_tag="RO454_004680"
     CDS             complement(2029..2265)
                     /gene="merE"
                     /locus_tag="RO454_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDY0936398.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            complement(2262..2624)
                     /gene="merD"
                     /locus_tag="RO454_004681"
     CDS             complement(2262..2624)
                     /gene="merD"
                     /locus_tag="RO454_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDY0936399.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            complement(2642..3139)
                     /locus_tag="RO454_004682"
                     /pseudo
     CDS             complement(2642..3139)
                     /locus_tag="RO454_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
     gene            complement(3300..4160)
                     /locus_tag="RO454_004683"
     CDS             complement(3300..4160)
                     /locus_tag="RO454_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDY0936400.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(4343..4900)
                     /locus_tag="RO454_004684"
     CDS             complement(4343..4900)
                     /locus_tag="RO454_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0936401.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            5064..5276
                     /locus_tag="RO454_004685"
                     /pseudo
     CDS             5064..5276
                     /locus_tag="RO454_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT         1155 a         1584 c         1493 g         1146 t
ORIGIN      
        1 tcggcgtagt agtggatgtg gtcgatgaca aagccggtgc gggtcagcgt gcgccggagg
       61 atcggcagaa aatcgaccag gaacgaagta gcgcgtgtga cgacggccgg tacgccgaca
      121 cgcgccacgg cctcggccca gcgcgcggcc ggcggttgga gcaggccgtt gtgcaccgaa
      181 ccgtggtagg tgccgaccgc caatgtgagc cagcgctcta gctcgcgcag cgtcagggcg
      241 gccttgtttt cggaatcgta gtcgccgcgc tggtcagggt tggagaaggt cgttcccggc
      301 agttcgtcgt gaatcatctg catcgccgtg ccgatgatcc gttccacgat gccgccatag
      361 tgcggctgtc ccagcgggcg atagtccagc cggatgccat gctgctcgca accccggcgc
      421 agggcctcgc tcttgaactc ggccgcgttg tctaggtaga gcagcaaggg cttgccgctc
      481 atctgccaat ccatttccac gttcagtcct tccagccaag ggcgcttgtc gcaggcgaca
      541 tgcacgaggc acaggccaac cgaaacggca gacggcgctt ccagcgtgac gaccatgccg
      601 agcacgcagc gggtgaacac gtcgatggcg agggtcaggt acgggcggcc aataggttgc
      661 cggtcgcggt catcgaccac gatcaggtcg atgaccgtat ggtctatctg cacctgctcc
      721 agcggcgcgg tcacggcagg aggctcgccg cccacacctt gtaggtcacg agcggcatcc
      781 tggccttccc gccggcggat gaccttgcgc gggtcaaggc tagcgatccg taaggccacg
      841 gtattgcgcg ccggcactcg cagtttttga gccttgcaca cctgagtgac ttcgcggtga
      901 aaggccgcta ggctgcgctt ctgcttggtc aggaaccgct tttgcagtag ctcgtggatg
      961 acgcgctcga ccggttccgg caagcgcccc ttacctttac ctccaccgga ctggccgggc
     1021 accagatccg tcacgaggcc gctgccttgc cgggcacgcc ggatcagaac gtatacctgg
     1081 cgccgagaca agcccagcgc ctgagccgcc atatcggccg cttcgtgccc gaccgtctcc
     1141 gactgcgcca acggactgat gatctccgca cgacggcgcg cacgctccca agcctcatca
     1201 ggcagagtgg ccacgccttg ttctggaatc cgtggggtgt ccgccgccat gctcacctcg
     1261 ctttggtgca cacgagtatt gagcatagtc gagattggtg cagatcactt ctgatattga
     1321 actgtcagga gctggctgca caacagccat tacgcccaat caactggtgc agtcgtcttc
     1381 tgaaaatgac atccatgccc agcccgtgcg cgagctggat caccgcccgc acgatagttt
     1441 ggtcacgggc atcatccggg agcctggcga caaaggattg gtcgattttc aatgtggtga
     1501 tggggcagca tttcagatgt tgcaggcagg aatagccggt gccgaagtcg tcggcggcga
     1561 agcgcacgcc gatggcgcgc aaggcgtcga aactggcgaa cagggctgga ttgccgaatg
     1621 cgaccgattc ggtcagttcg atctccagaa gctcggcggg cagggccata tcggccagca
     1681 cccgctttac ctcgtcgtcg aacgttggcc caacctggct ggcggacaca ttgatggcaa
     1741 gacggaacgg ttgccatgcc ggtccttgcc acttgtgcat ctggcgacag gcctcgccca
     1801 gcacccacgc gcctatttcc ggcatcaggc cgaacgactc ggccagcggc aggaactggc
     1861 cgggcggcaa caggccaagc ctcggatgcc gccagcgcat caacgcttcc gcgccagcga
     1921 tccggtgatc gcgcagatcg accagcggct ggtaatgcag gtcaagctgt ccgcgcgccg
     1981 ccgcctgcgc caactcggcc gccgtccatc cggcgggctg cgaactcgtc atgatccgcc
     2041 ccggaaggcg cgcagcagcc gcgttacggc cagaacgaac aagccggtca gcgcgagcgc
     2101 ggcaacaccc caatgctcgc caaggaaggc accggcggtc gtcccggcca gcacggcggc
     2161 gagaatcggc agatggcagg ggcaggtcaa cacggccagc gcaccccaca ggtagccgga
     2221 aacgggttgg cgcgtctcgg gcggcagttt gtcaggggcg ttcacggcaa tgcctcctcg
     2281 tgcgcccgct cggctggcat ggaggccagt tgcgcgtcca gatgggccaa cgccgcgcgc
     2341 cgccgctcga ccaactggcg cagcacggca agctgcgctg cggcttgtgc gccgtccgct
     2401 gcgtcgagcg cacggcacag ccgcgccagg gcatccaggc cgatacccgc ctcgaaggcc
     2461 gcgcgcacga agcacagccg ttgcaaggcc gcatcgtcga acacgccgta gccgcccgtg
     2521 gtgcaggcca ccggccgtaa caagccgcgc accaggtagt cgcgcacgat atgtacgctc
     2581 accccagcgt tatgggccag ttgcgatacc gtgtaggcgc tcatcgcaca cctccttgtc
     2641 ctcacccggc gcagcaggaa agctgcttca catccttgtt gaaggtctgc gccgcgagct
     2701 tcaacccttc gaccatcgtc aggtagggga acaactggtc ggccagttcc tgcaccgtca
     2761 tccggttgcg aatcgccagt gcggccgtct ggatcagttc gcccgcttcc ggggccactg
     2821 cctgcacgcc gatcagtcgt ccgctgcctt cttcaaccac cagtttgatg aagccgcgcg
     2881 tgtcgaagtt ggcgagcgcg cgcggcacgt tgtccagcgt tagcgtgcga ctatcagttt
     2941 tgatgccgtc atggtgcgct tccgcctcgc tgtagcctac ggtcgccact tgcgggtcgg
     3001 tgaacaccac ggccggcatc gcggtcaggt tcagggccgc gtcaccgccg gtcatgttga
     3061 tcgcggcgcg agtgccggcc gctgccgcca catagacgaa ctgcggctgg tcggtgcagt
     3121 cgcctgcggc gtagatgtgt tccacgcttg taggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggcagcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cgaaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttaccagggt
     4201 tattgtatca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4321 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ttataaaccg
     4381 tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc ttctgatgaa
     4441 gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt cctttcagct
     4501 ttgcttcctg tcggccctca ttcgtgcgtt ctaggatcct ccggcgttca gcctgtgcca
     4561 cagccgacag gatggtgacc accatttgcc ccatatcacc gtcggtactg atcccgtcat
     4621 caatgaaccg gactgccacg ccctgagcgt caaattcctt tatcagttgg atcatatcgg
     4681 cagtgtcgcg gccaagacgg tcgagcttct taaccagaat gacatcacct tcctccacct
     4741 tcatcctcag caaatccagc ccttcccggt ctgttgaact gccggatgcc ttatcggtaa
     4801 atatacggtt tgctttcaca cctgcgtctt tgagtgctct gacctgaaga tcaagagact
     4861 gctgactggt tgagacccga gcgtaaccaa aaagtcgcat aaaaatgtac cttaaatcga
     4921 atatcggaca actcatgtct attattacaa atttacgatt taatagacat attaatgtaa
     4981 cagttttacg atgtccgata atttataaca tttcgtacgg ttggaaaaat gttactaaat
     5041 gcccgtcagg cagggaggcc gatatgcccg ttgactttct gaccactgag cagactgaaa
     5101 gctatggcag attcaccggt gaaccggatg agcttcagct ggcacgatat tttcaccttg
     5161 atgaagcaga caaggaattt atcggaaaaa gcagaggtga tcacaaccgt ctgggcattg
     5221 ccctgcaaat tggatgtgtc cgttttctgg gcaccttcct caccgatatg aatcatacct
     5281 agattctacg tcagtacttc aaaaagcata atcaaagcct tgataaatat gcattccttc
     5341 gaaattcagc tttcacccat tgggtgaaag aaaagtgc
//
LOCUS       DAPQZD010000245         5340 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPQZD010000245 DAPQZD010000000
VERSION     DAPQZD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.247"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1784
                     /locus_tag="RO454_004686"
     CDS             <1..1784
                     /locus_tag="RO454_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HDY0936402.1"
                     /translation="RKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAIIVDPYGN
                     APLTALVDLDSHVISDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQKFANK
                     VTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLYLVNTH
                     TFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQNTFY
                     DGRDRNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHES
                     IETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDAL
                     LGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSII
                     LSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSD
                     FDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVW
                     EYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTIGKLNEIDYKTKEVK
                     VEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1802..2470
                     /locus_tag="RO454_004687"
     CDS             1802..2470
                     /locus_tag="RO454_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HDY0936403.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2485..3156
                     /gene="dsbI"
                     /locus_tag="RO454_004688"
     CDS             2485..3156
                     /gene="dsbI"
                     /locus_tag="RO454_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HDY0936404.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGTLIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3202..3990)
                     /gene="ygiD"
                     /locus_tag="RO454_004689"
     CDS             complement(3202..3990)
                     /gene="ygiD"
                     /locus_tag="RO454_004689"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDY0936405.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4133..4906
                     /gene="zupT"
                     /locus_tag="RO454_004690"
     CDS             4133..4906
                     /gene="zupT"
                     /locus_tag="RO454_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDY0936406.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4968..5138)
                     /locus_tag="RO454_004691"
     CDS             complement(4968..5138)
                     /locus_tag="RO454_004691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936407.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1398 a         1298 c         1289 g         1355 t
ORIGIN      
        1 atagaaaaac actcgtagcc ggaactgtgg cgataaccct gggtttgtca gcatcggggg
       61 tgatggctgc gggttttaaa ccagcaccgc ctgccgggca gcttggggcg attatcgtcg
      121 atccctacgg taatgcgccc ttaaccgcgc tggttgattt agacagccat gttatttctg
      181 acgtcaaagt taccgtccac gggaagggcg aaaaaggcgt tgacatcagc tataaagtgg
      241 gcaacgagtc actgaaaact tacgatggtg taccgatttt tggtctttac cagaagtttg
      301 cgaacaaagt gaccgttgag tggaaagaaa acggtaaggc catgaaagat gattacgtgg
      361 tgcagacttc cgccatcgtc aacaattaca tggataaccg ctccatctcc gacctacagc
      421 aaaccaaagt cgttaaagtc gcgccaggtt ttgaagatcg cctctatctg gttaataccc
      481 acacctttac cgcccaaggt tccgatctcc actggcatgg tgagaaagat aaaaatgccg
      541 gtatccttga tgcgggtccg gcaactggcg cactcccttt tgatatcgcg ccattcacct
      601 ttatcgtcga tacggaaggc gaataccgct ggtggttgga tcaaaacacc ttctacgatg
      661 gtcgtgaccg caacattaac aaacgtggtt atctgatggg tatccgtgaa acgccacgcg
      721 gcacctttac cgctgtacaa ggtcagcact ggtacgagtt cgacatgatg gggcaggtgc
      781 tcgaagatca caaactaccg cgcggatttg ctgacgccac tcatgagtcc attgagacgc
      841 caaatggcac ggtactgttg cgcgtaggta agagtaacta tcgtcgcgat gacggcgtac
      901 acgtcaccac cattcgtgac catatcctcg aagtcgataa atctggtcgc gttgtcgatg
      961 tatgggatct gaccaagatc ctcgatccga aacgcgatgc actgctcggc gcgctggatg
     1021 caggtgcagt ttgcgttaac gttgaccttg cccatgcagg acaacaggca aaactggaac
     1081 cagatacacc gttcggcgac gcgctgggtg tagggccagg ccgcaactgg gcgcacgtga
     1141 actctatcgc ctatgacgca aaagatgact ctatcatcct ctcctctcgc caccagggtg
     1201 ttgtgaagat tggtcgtgat aagcaagtga aatggatcct tgcgccctct aaaggttggg
     1261 aaaaaccgct ggccagcaag ctgctgaaac cggttgatgc taacggtaag ccaattacct
     1321 gtaacgaaaa tggcctgtgc gaaaactcag acttcgactt tacctacacc cagcataccg
     1381 cctggatttc cagcaaagga acgctcacca tttttgataa tggcgatggg cgtcatctgg
     1441 aacaacctgc cttaccaacc atgaaatact cccgctttgt ggaatataag attgatgaga
     1501 agaaaggcac cgttcagcaa gtgtgggaat acggtaaaga acgtggctac gatttctata
     1561 gcccgattac ctccatcatt gaatatcaag ccgaccgtaa caccatgttt ggcttcggtg
     1621 gttctattca tttgttcgat gtcgggcagc caaccatcgg taagttgaac gaaatcgatt
     1681 acaaaaccaa agaagtgaaa gtggaaatcg acgtgctgtc agataaaccc aatcagactc
     1741 actatcgtgc actgttagtc cgtccacaac agatgttcaa ataattaaga agggaataaa
     1801 aatgtcgaaa ttagggatct catcactatt taaaaccata ctgcttaccg cagctttggc
     1861 tgtttcattt accgcttctg catttaccga aggcacggat tacatggttc tggaaaaacc
     1921 tattccgaat gccgacaaaa cgctgattaa ggtatttagc tacgcctgcc cgttctgtta
     1981 caagtacgac aaagcggtta ccggtccggt atcggaaaaa gtgaaagata tcgttgcttt
     2041 cacgccgttc catctggaaa ccaaaggtga atacggcaaa caagcaagcg aagtctttgc
     2101 ggtcttgatc aataaagata aagcagcagg catttcctta tttgatgcta actctcagtt
     2161 taagaaagcc aagtttgcct actacgcggc ttatcacgac aaaaaagaac gctggtctga
     2221 cggtaaagat cccgctgcct ttatcaaaac tggtctggat gccgcaggta tgagtcaggc
     2281 ggattttgaa gcggcactga aagaaccagc agttcaagaa acactggaaa aatggaaagc
     2341 ctcttatgat gtcgccaaaa tccagggggt tccagcctat gtcgttaatg gtaagtactt
     2401 gatctacacc aagagcatca aatccatcga cgcgatggca gaccttatcc gtgagttagc
     2461 aagcaaataa cagggaaaca ggctatgggt attaagggaa tgtggaaaga tcttcgcaca
     2521 tcaccagttg acacactggt cagatggcag gagcaacgct tgttgtggtt gttaatggcc
     2581 gttgcgatgg gcacgttaat tatcctggcg cactctttct tccagatcta tctctacatg
     2641 gcaccctgtg agcaatgtgt atacattcgc tacgccatgt ttgtgatggt gattggagga
     2701 ttggttgcag cgatcaatcc aaagaacatc atcctcaagc taattggctg cgtgatggca
     2761 ttttacggca gcattttggg actgaagttt tcgctcaaac tgaacgatat ccaccatgca
     2821 gtacataatc cggatcccga ttcactgttt ggcgtacagg gttgctctac cgatcccact
     2881 tttcccttta acctgccgct ggcacagtgg gcaccaaatt ggttcaagcc taccggggat
     2941 tgtggctatg acgcaccaat cgttcctgat ggcgtcacac tgagcagcac gcaacagtgg
     3001 tttgtggaaa tgtatcagca atctgaaggc tggtatctgc taccaccatg gcattttatg
     3061 aatatggctc aggcttgtat gctggctttt ggtatgtgtc tggtgctgct ggtcattatg
     3121 agtggcgcat gggcgcttaa aatcatcaga ggctaactca gcaacacccg aagccagtca
     3181 ttctggcttc gggttctttt attaacctat ctgcaccgac agcatactta aactgcccat
     3241 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacgccc ctaagacata
     3301 caacaacggc agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag
     3361 ataattcacc agaggatgtt gttccaccgg cccttgccac gtcagattcg ctttcacata
     3421 ctcattaaac gacatcgccc acggatacgg tgaactatca ccgtgccact tcactgtacg
     3481 caggttatgc accacgttgc cactggcgac caacattatc ccttcatcac gcagcgccgc
     3541 gagtttgcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
     3601 caccatcggg atatcggcgt caggatacat cttaatcagt acgccccacg agccgtggtc
     3661 gaagccccag gcttctttat ccagcgtcac agggaccggc gctaacagct caaccagatg
     3721 ctgtgccagc gcaggtgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
     3781 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
     3841 aaaccaatga gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt
     3901 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
     3961 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
     4021 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
     4081 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
     4141 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
     4201 tctcgggcaa aaaccctcga accgcttact ggcgttttcg ctaggatttg cggcggggat
     4261 catgttgctc atctcattaa tggaaatgct tcctgccgca ttagccgccg aaggaatgtc
     4321 acctgtgttg ggttatggaa tgtttatctt cggtctgctt ggctattttg gtctggaccg
     4381 catgttgcca catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa
     4441 atcgatcaag cgcacagcca ttctgctcac tctcggtatc agtctgcata acttcccgga
     4501 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gtatcgccct
     4561 ggccgtagcg ttgcacaata ttcctgaagg tctggcggtt gcaggcccgg tttacgcggc
     4621 aacggggtca aaacgtaccg caattctgtg ggcggggatt tccggactgg ctgaaattct
     4681 cggtggtgtg ctggcatggt taattcttgg cagcatgatt tctccggtgg tcatggcggc
     4741 aatcatggcg gcggttgccg gtattatggt ggcgctctcg gttgatgaat taatgccgct
     4801 cgccaaagag attgacccca ataataaccc cagttatggc gtgctatgtg gaatgtcagt
     4861 gatgggattc agtttagtgc tgctacaaac ggcaggaatt ggttaaagat gataatgaca
     4921 gatgttgccc attgctaaag gcaacatctg tatcgttatt aaaggtatta ttgatgcttt
     4981 tcaaacactt ctttatcgga gcgtttatct aatttacttc gggcagctaa cagcgcatct
     5041 ctgtcatggc gaaaagcttt acagtaacgt ttaagatgaa gaaaatagcc accagctagt
     5101 agaacaatta atacaatcca gtaccaggca ataaacatcc ttttcacctt caaaatttaa
     5161 cattactaaa catttagcgt taaaatttca catatccaac gttgaatata ttcatatact
     5221 cgcatagttg tggatcatgt atatgatttc gatcatagta agtgttgtat tatatctcat
     5281 gattgttcaa aaaacaatcg atcgataata ttgattatgt atcaattctc cataaaacat
//
LOCUS       DAPQZD010000246         5332 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPQZD010000246 DAPQZD010000000
VERSION     DAPQZD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.248"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..66
                     /locus_tag="RO454_004692"
     CDS             <1..66
                     /locus_tag="RO454_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDY0936408.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
     gene            82..705
                     /locus_tag="RO454_004693"
     CDS             82..705
                     /locus_tag="RO454_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HDY0936409.1"
                     /translation="MLKIIKQHKFAFLFTALLSTFFLNMFPPSAAGYELNTVLQIVAG
                     VNLLQRRYQVVSFIVLLIMFVTTHWLHPVNAMIQLYYGSYLVFFIILSVIVFRQVIFS
                     ARINTESICAALSGFLLIGYIGFFMFSAIENHMPGAFRGLSNDELQMMNELFYYSFIS
                     ILTVGYGDIVAVSWPARNATILVILTGYIYSLVFIARIVSGFSVSEK"
     gene            790..1041
                     /locus_tag="RO454_004694"
                     /pseudo
     CDS             790..1041
                     /locus_tag="RO454_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(1330..1647)
                     /locus_tag="RO454_004695"
                     /pseudo
     CDS             complement(1330..1647)
                     /locus_tag="RO454_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1802..1907
                     /locus_tag="RO454_004696"
                     /pseudo
     CDS             1802..1907
                     /locus_tag="RO454_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="PsiA; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            2139..2297
                     /locus_tag="RO454_004697"
     CDS             2139..2297
                     /locus_tag="RO454_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDY0936410.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAA
                     FMAYESGK"
     gene            2965..3171
                     /locus_tag="RO454_004698"
     CDS             2965..3171
                     /locus_tag="RO454_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDY0936411.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPRQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            3196..3483
                     /locus_tag="RO454_004699"
     CDS             3196..3483
                     /locus_tag="RO454_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936412.1"
                     /translation="MSTQNIHVSTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            3602..4423
                     /locus_tag="RO454_004700"
     CDS             3602..4423
                     /locus_tag="RO454_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDY0936413.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRAQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(4719..5291)
                     /locus_tag="RO454_004701"
     CDS             complement(4719..5291)
                     /locus_tag="RO454_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDY0936414.1"
                     /translation="MLSLFVEWVKLFTDKVTRGGKMKKWMLSICLMFINEICHATDCF
                     DLAGRDYKIDPDLLRAISWKESRYRVNAVGINPVTGYGSGLMQVDSQHFNELARYGIK
                     PEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQRRLAYASDVY
                     RIYTGIKSSKGIRIPVTKKSLTEINSVQNN"
BASE COUNT         1286 a         1268 c         1339 g         1439 t
ORIGIN      
        1 tcaaaatcgg tggagctgca tgacaaagtc attgggcatt atctgaacat aaaacactat
       61 caataagttg gagtcattac catgttaaag ataataaaac aacacaaatt tgcttttctt
      121 ttcacagcac tgctgagcac cttctttctc aatatgtttc caccgagcgc tgcagggtac
      181 gagttgaata ccgtgctgca gattgtggcg ggcgttaatc tgttgcagcg tcgctatcag
      241 gtcgtttcat ttattgtgtt gctgataatg tttgtcacca cacactggct gcatcccgtc
      301 aatgccatga tacagctgta ctacgggagt tatctggtct ttttcatcat tctgtcagtg
      361 attgtgttcc gccaggtgat tttctccgcc cggataaaca cagagtccat ctgcgctgca
      421 ctaagtggct ttctgcttat tggctatata gggtttttca tgttttctgc gatagaaaat
      481 catatgcctg gcgcgttccg tggactgtca aatgatgagt tgcagatgat gaatgaactt
      541 ttttattatt cttttatcag tattctgacc gtaggctatg gcgatattgt tgccgtcagc
      601 tggccggctc gtaatgccac tattctggtt attctcacgg gctatattta ctcactggtc
      661 tttattgccc gcatcgtcag tggattttcg gtctcagaaa aataactctt tgtaccagct
      721 accagtcggc tgtactacgg gttcctgagt ccggcaaaac ggcatcgtgt atcgttccgg
      781 tcgagtaggc agcctaaccc gaccactgaa aacgtcgcgg cagagctgaa gctgctgacg
      841 gaaaattgct gcgatagtgg gatttctctg ggaggtggat cgccgcacga ctgcgcgaaa
      901 ggaattgcgt tggtcgcaac caatggtgga aatattcgtg attatgaagg tgttgatcgt
      961 tccgcgaaac cgctgctgcc aatgattgcg attaacacca ctgcgggtac ggcttcggaa
     1021 atgacccgtt tctgtattat ggggtcagtt tggatatcgg aacttattgt actataaggg
     1081 cttccccggc atgtcaacgc cggttttata gtttctgtct gctcgtcagt aaaataaagg
     1141 ggagagactg cggtactata aacggccatg atgaatcgtt atcgattcgg gccctgatga
     1201 aagacgcgcg tgaggttctt gttcgttaag cggatacgta tatcctgtac aatagccaga
     1261 taggcctcac ggggtctaac cctttttcat caacgagttg caggtcagat ggttatactg
     1321 ctttgtgctt tagctggctg agtacagcct gaaatcatca ccgtaatgca acgatgccca
     1381 gcacagcctc gcgtttttgg cggcgatggc tacaacagcc cgccagtacc ccctgcgctc
     1441 aaccaacgta caaacccaac ggctgaatga gtcagtcctt ttctctgcac caatcaaaac
     1501 cgaacgggcc ccctgaacca gaagcgttcg cagatatgaa tcgcccgctt tcgttattct
     1561 gccaagcttt gattttccgc cactgctgta ctgtgatggt gtaagtccca gccaggctgc
     1621 cagttggcgt ccattcttaa aatcatggcg ttgacaaggc aagtatggca gccagagagg
     1681 ccggttgcgt attttcatga aaggagatca gtgaaggtaa tttaaaaacg gttctgtgaa
     1741 agtgacacga accgatctcc atcgatgtta ctcaccgatc tccttcacgg taatacgcac
     1801 cggtcacgct gtccgtgaga tggagcggtg gatggtgccg aacaaactcc gggaggcagc
     1861 gtgatgcggc aacaatcaca cagattaccc gtaaacagtc tgaatgagcg ggttattttc
     1921 aggaaaagtg agtgtggtca gcgtgcaggg atatgggcta tgatgtgccc ggcgcttgag
     1981 gctttctgcc tcatgacgtg aaggtggttt gttaccgtgt tgtgtggcag aaggacaaaa
     2041 gccccgtagt taatttttca ttaacccacg aggcccctgc atgcttagac aacatcagga
     2101 tagcctctta ccgcgctttg cgcaaggaga agaaggccat gaaactacca cgcagctctc
     2161 ttgtctggtg tgtgttgatc gtgtgtctca cactgttgat attcacttat ctgacacgaa
     2221 aatcgctgtg cgagattcgt tacagagacg gatacaggga ggtggcggct ttcatggctt
     2281 acgaatccgg taagtagcaa cctggaggcg ggcgcaggcc cgccttttca gggctgatgc
     2341 tggtctgact gcactgaagc gcctttataa aggggttgct ggttcgccgg tggccccttt
     2401 ctccttgctg gcattgtacg ggcatgaaca accagactgc caggaaggcc atttccctcc
     2461 gggaacgggg atgaacgtgc gcctgcggcg cccgttcttt tcccccgcct tctctgttta
     2521 tgacctgtca gaaatcacat caccttccct ttgattattc tctttttcct cctgttctga
     2581 ctctgattac tgctgccctg attattgtct gttttgttgt tctcctgctt atgccttttc
     2641 cgggcgtccc cctggcgggg cgggctgtcg tgaacggagc cagcgaagct gtctccgccc
     2701 tggcgggctt ccatcccgga cgcgaaaccc cgggcttgca gcgaccgttc cggtcgcgcc
     2761 gcctctgtcg cgccgtggcc tgcgctcctt acatcccggc aaaaggccgc cgggatgtaa
     2821 ggcgcaacgc cgtttcgcaa accgcgcttc acgtcgttgc ggcgggtgcc tgcccgttgt
     2881 tgtgtccggt gccattcacc tgtttaagcc cctgtcgccg ggggcaaaga cagccacaac
     2941 cgtctccgcc agtccagggg tgaaatgtac atcccgcaaa actttttgcc caaaagcagg
     3001 caaaaacttt tgcggtctgc ccccagacag tcgtttccgg ccgtggcagt gatgcccctt
     3061 gcggcgaagg ttctcaaaaa ggtgatggtg aataccggag ggaacaaaag gcaggtcccc
     3121 gcaaaacgtc aaaaccgcgg ctcccgaaac tcaaaagatg atgggggctg aaaaccagag
     3181 agaggagaaa gcaggatgag tacccaaaat atccacgtta gcaccgcgtc gtacacgctt
     3241 cttgttgccg ggaagaaaaa aaacaccggg gaagagtggg acgtcctaga attcagcagc
     3301 ctgactgagc tgaaaaaata tcgcaaaagc cacccggaaa agatggcctt cagctatagc
     3361 tacgcgctca gccggggtgt ggatacgcag ttccgccata tcaacattgc ggaagccgat
     3421 cacttcaaac agttcctgcg tcagattaag cgtgccggcc tcgatatccg ggcgatctgc
     3481 tgaccggatg tgatgaatat ccctcctgat tgttgctgac ttgttaatga tggtagggag
     3541 ggatgatatt aagcggaaaa attttgtatt aaatttattc tttcataaag gagtagctgt
     3601 tatgcgatta gcttcccgtt ttggtcggta taattccatc cgccgtgaac gtcctttaac
     3661 ggatgatgaa ttaatgcagt tcgtgccttc ggtattttcc ggtgataaac atgagtcccg
     3721 gagtgaacgt tatacgtata ttccaacaat caatatcatc aataagttac gtgatgaagg
     3781 tttccagcca ttctttgcct gtcagagtcg ggttcgtgat ttgggacgtc gcgaatacag
     3841 taaacatatg ttacgtcttc gcagggaagg gcatattaac gggcaggaag ttcctgaaat
     3901 tatcctgctt aattcacatg atggttcatc cagttatcag atgatccccg gaatttttcg
     3961 ttttgtctgc acaaatggcc tggtgtgcgg aaataatttt ggcgaaatcc gcgttccaca
     4021 taaaggtgat attgtcgggc aggttatcga gggagcgtat gaagtgctcg gtgtctttga
     4081 taaggtcact gataatatgg aggcgatgaa agaaattcat cttaacagtg acgagcaaca
     4141 tttatttggt agagctgcac tgatggtcag gtatgaagat gaaaataaaa cgccagtgac
     4201 gcctgaacaa ataattactc cccgtcgtcg ggaggataaa cagaacgatc tctggacaac
     4261 ctggcagcgg gctcaggaga atatgataaa aggtggatta tcggggcgaa gtgcctccgg
     4321 gaaaaatacc aggacaagag ccattacagg tattgatggt gatataagga tcaacaaggc
     4381 gttgtgggtg attgctgaac agttcagaaa gtggaagtca tgataatata agtaaagccc
     4441 cgaaaaattt tcggggcttt acttatatta tcattcttcg gcatcgctgt gtcgtctgat
     4501 atacatcaat ttcatcaact ggctttccgg ttgtctgtaa atattgtgcc cgcgaaaaat
     4561 ggtctgacag gaggctgcgc agttaccggg tatatataat atgggttccc gcttcgcggg
     4621 acttcggcca gtcagcatta tcactcatat gatatttttg tgtgtggcct tccatgccgc
     4681 ttttgcggca taaccggtat ctgacaatgt ctgtaagatt aattattctg cacgctgtta
     4741 atttccgtaa gtgatttctt tgtgaccgga atccggatac ctttactgct ttttatcccg
     4801 gtataaatcc ggtaaacatc tgatgcgtag gcaagacgtc tctggttctg gcgttcggtt
     4861 ttcctgaatc ctgcattata tgcaccaacg gcctcccagg tgacgcccca ttttttaaat
     4921 gctattgcca gataataagc accggtataa atgttcatgc agggatctgt tgtcagatgt
     4981 tccggcttaa ttccgtagcg ggccagttcg ttaaaatgct gggaatctac ctgcatcagt
     5041 ccgctgccat atcccgttac cggattaata ccgacggcat taacccggta acgggattct
     5101 ttccatgata ttgctctcag taaatcagga tctattttgt aatcccgtcc tgcaagatca
     5161 aagcaatcag tggcatggca gatctcattt ataaacatca ggcagatgga taacatccat
     5221 tttttcattt ttccacctct ggtgacttta tccgtaaata atttaaccca ctccacaaaa
     5281 aggctcaaca tgttggtggt tctcaccacc aaaagcacca caccccacgc aa
//
LOCUS       DAPQZD010000247         5211 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPQZD010000247 DAPQZD010000000
VERSION     DAPQZD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.249"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..790
                     /gene="mhpR"
                     /locus_tag="RO454_004702"
     CDS             <1..790
                     /gene="mhpR"
                     /locus_tag="RO454_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDY0936415.1"
                     /translation="TRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLLEEG
                     YVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDA
                     MVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDD
                     YQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMA
                     SAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            867..1949
                     /gene="lacI"
                     /locus_tag="RO454_004703"
     CDS             867..1949
                     /gene="lacI"
                     /locus_tag="RO454_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDY0936416.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            2072..5146
                     /gene="lacZ"
                     /locus_tag="RO454_004704"
     CDS             2072..5146
                     /gene="lacZ"
                     /locus_tag="RO454_004704"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDY0936417.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFL
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
BASE COUNT         1176 a         1411 c         1507 g         1117 t
ORIGIN      
        1 aacccgcggt ctaatgttat taaatatgtt aaataaactt gatggcggtg ccagcgtcgg
       61 gctgctggcg gaactcagcg gcctgcatcg caccactgtg cggcgactgc tggagacgtt
      121 actggaagag ggatatgtcc ggcgcagtac ctccgatgac agttttcgtc tgaccatcaa
      181 agtgcggcaa ttaagcgaag gatttcgtga cgaacagtgg atttctgcac tggcggcccc
      241 gctgctgggc gatctgttgc gcgaagtggt atggccgaca gatgtgtcca cgctggatgt
      301 tgatgcaatg gtggtacgcg aaaccaccca tcgtttcagc cgcttatctt ttcaccgggc
      361 aatggtcggg cgacggttac cgcttctgaa aaccgcctcg ggcctgacct ggctggcctt
      421 ttgcccggaa caagaacgca aggaattaat cgagatgtta gcctcccgcc ccggtgatga
      481 ctatcaactg gcacgagaac cgttaaagct ggaagccatt ctggcgcgcg cgcgcaaaga
      541 ggggtacgga cagaactacc gcggctggga tcaggaggag aagatcgcct ctatcgccgt
      601 accgctgcgc agtgaacaac gggtgattgg ctgtctgaat ctggtgtata tggcgagcgc
      661 aatgaccatc gaacaggcag cggaaaagca tcttccggcg ctgcaacggg tggcaaaaca
      721 gatcgaagaa ggggttgaat cgcaggctat tctggtagcg ggaaggcgaa gcggcgtgca
      781 tttacgctga caccatcgaa tggcgcaaaa cctttcgtgg tatggcatga tagcgcccgg
      841 aagagagtca attcagggtg gtgaatgtga aaccagtaac gctatacgat gtcgcagagt
      901 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggcctgc cacgtttcag
      961 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     1021 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccact tccagtctgg
     1081 ccctgcacgc gccgtcgcaa attgttgcgg cgataaaatc tcgcgccgat caactgggtg
     1141 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     1201 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     1261 atgccattgc tgtggaagct gcctgcgcta atgttccggc gttatttctt gatgtctctg
     1321 accagactcc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     1381 agcacctggt cgcattgggt caccagcaaa tcgcgctgtt agcaggccca ttaagttctg
     1441 tctcggcgcg tctgcgtctg gcgggctggc ataaatatct cacacgcaat caaattcagc
     1501 cgataacaga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     1561 tgctaaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     1621 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     1681 gatacgacga taccgaagac agctcgtgtt atatcccgcc gttaaccacc atcaaacagg
     1741 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     1801 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc acccttccgc
     1861 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1921 aagtttcccg actggaaagc gggcagtgag cgcaacacaa ttaatgtgag tcagctcact
     1981 cattaggcac cccagtcttt acactctatg tgtccggctc gtatgttgtg tgaaattgtg
     2041 agcggataac aatttcacac aggatacagc tatgactatg attacggatt ctctggccgt
     2101 cgtattacaa cgtcgtgact gggaaaaccc tggcgttaca caacttaatc gccttgcggc
     2161 acatccccct ttcgccagct ggcgaaatag cgaagaggcc cgcaccgatc gcccttccca
     2221 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     2281 gccggaaagc tggctggagt gcgatcttcc tgacgccgat actgtcgtcg tcccctcaaa
     2341 ctggcagatg cacggttacg acgcgcccat ctacaccaac gtaacctatc ccattacggt
     2401 caatccgccg tttgttcccg cggagaatcc gacaggttgt tactcgctca catttaatgt
     2461 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
     2521 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagcc gtttgccgtc
     2581 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
     2641 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
     2701 ccgtgacgtc tcgttgctgc ataaaccgac cacgcaaatc agcgatttcc aagttaccac
     2761 tcgctttaat gatgatttca gccgcgcggt actggaggca gaagttcaga tgtacggcga
     2821 gctgcgcgat gaactgcggg tgacggtttc tttgtggcag ggtgaaacgc aggtcgccag
     2881 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggcggttatg ccgatcgcgt
     2941 cacgctacgc ctgaacgttg aaaatccgga actgtggagc gccgaaatcc cgaatctcta
     3001 tcgtgcggtg gttaaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
     3061 tgtcggtttc ctcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
     3121 gctgattcgc ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
     3181 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaatg ccgtgcgctg
     3241 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgttacg gcctgtatgt
     3301 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
     3361 tccgcgctgg ctacccgcga tgagcgaacg cgtaacgcgg atggtgcagc gcgatcgtaa
     3421 tcacccgagt gtgatcatct ggtcgctggg gaatgagtca ggccacggcg ctaatcacga
     3481 cgcactctat cgctggatca aatctgtcga tccttcccgc ccggtacagt atgaaggcgg
     3541 cggagccgac acctccgcaa ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
     3601 ccagcccttc ccggcggtgc cgaaatggtc catcaaaaaa tggctttcgc tgcctggaga
     3661 aatgcgcccg ctgatccttt gcgaatatgc ccacgcgatg ggtaacagtc ttggcggctt
     3721 cgctaaatac tggcaggcgt ttcgtcagta cccccgttta cagggcggct tcgtctggga
     3781 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg
     3841 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc
     3901 cgaccgcacg ccgcatccgg cgctgacgga agcaaaacac caacagcagt atttccagtt
     3961 ccgtttatcc gggcgaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
     4021 cgagttcctg cactggatgg tggcgctgga tggcaagccg ctggcaagcg gtgaagtgcc
     4081 tctggatgtt ggcccgcaag gtaagcagtt gattgaactg cctgaactgc cgcagccgga
     4141 gagcgccgga caactctggc taacggtacg cgtagtgcaa ccaaacgcga ccgcatggtc
     4201 agaagccgga cacatcagcg cctggcagca atggcgtctg gcggaaaacc tcagcgtgac
     4261 actcccctcc gcgtcccacg ccatccctca actgaccacc agcgaaacgg atttttgcat
     4321 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg
     4381 gattggcgat gaaaaacaac tgctgacccc gctgcgcgat cagttcaccc gtgcgccgct
     4441 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
     4501 ctggaaggcg gctggccatt accaggccga agcggcgttg ttgcagtgca cggcagatac
     4561 acttgccgac gcggtgctga ttacgaccgc ccacgcgtgg cagcatcagg ggaaaacctt
     4621 atttatcagc cggaaaacct accggattga tggacacggc gagatggtcg tcagtgtagg
     4681 tgttgaagta gcgagcgata caccgcatcc ggcgcggatt ggcctgacct gccagctggc
     4741 gcaggtcgca gagcgggtaa actggctcgg cctggggccg caagaaaact atcccgaccg
     4801 ccttactgca gcctgtttta accgctggga tctgccattg tcagacatgt ataccccgta
     4861 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
     4921 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agccaacaac aactgatgga
     4981 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
     5041 ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct
     5101 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
     5161 gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa a
//
LOCUS       DAPQZD010000248         5200 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPQZD010000248 DAPQZD010000000
VERSION     DAPQZD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.250"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            302..430
                     /locus_tag="RO454_004705"
                     /pseudo
     CDS             302..430
                     /locus_tag="RO454_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(465..758)
                     /locus_tag="RO454_004706"
     CDS             complement(465..758)
                     /locus_tag="RO454_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936418.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGARTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKCISSDLI"
     gene            complement(864..1139)
                     /locus_tag="RO454_004707"
     CDS             complement(864..1139)
                     /locus_tag="RO454_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDY0936419.1"
                     /translation="MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIYVLRLWHTRENR"
     gene            complement(1139..1423)
                     /locus_tag="RO454_004708"
     CDS             complement(1139..1423)
                     /locus_tag="RO454_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDY0936420.1"
                     /translation="MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWSDSLSTDNPLPVPR"
     gene            2028..2780
                     /locus_tag="RO454_004709"
     CDS             2028..2780
                     /locus_tag="RO454_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDY0936421.1"
                     /translation="MIATKTPAIDKAARIFHFIAGKGSATYSEIHQNVGLPQSTTSAL
                     LASLTTHAFLRQKNGRYYLGLIFYELGNKAIQDFDFRELAEESLVFLRDQTRLACHLG
                     VLDGSTGIYLAKKESQNAIIVRSWTGKRLSLHSSSLGKVLLAWLPEDHIDALLPTEHL
                     PAFTARTITTRKALKAELEVVRLQGWAFDNQEDVDDVTCIAAPVFNSDQAVVAAISIS
                     GVRFQMPEEKIPEYANAVMKAAGMMSEKLCGI"
     gene            complement(2826..3791)
                     /gene="kdgT"
                     /locus_tag="RO454_004710"
     CDS             complement(2826..3791)
                     /gene="kdgT"
                     /locus_tag="RO454_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDY0936422.1"
                     /translation="MNIKKTVEKIPGGMMLAPLFLGAVLHTFWPGTGKYFGSFTNGMI
                     TGVVPILAVWLFCMGASIKISATGTVLKKSGTLVATKIATAWVCAFVFAQLLPEGGMV
                     KTGFFAGLSVLAIVAAMDMTNAGLYASLMQEYGTKEEAGASVLISLESGPLMTMIILG
                     SAGQATFEPEHLAGVLIPFLVGFLLGNLDPELRELFSRATKSLIPFFAFALGNTINLG
                     VIIDTGLLGILMALAVIVITGVPLIIMDIMLGKGRGTAGIAASSTAGAAVATPLLVAE
                     IAPDFAEAAPAATTLVASCVVITAIVVPVITALWAKHGASRVRAT"
     gene            complement(3824..4204)
                     /locus_tag="RO454_004711"
     CDS             complement(3824..4204)
                     /locus_tag="RO454_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDY0936423.1"
                     /translation="MKKDIIYTTGAPAPGGALSQAVKAGETIYLAGQVGFDPHTMQVV
                     STDFDEQARQAFKNLLSVAEAAGGSDRDIVKLNAYLTDVNMFPRFNAIMSEYFSPPYP
                     ARATLGIAALPQGAVIEIEAIMVL"
     gene            complement(4229..5119)
                     /locus_tag="RO454_004712"
     CDS             complement(4229..5119)
                     /locus_tag="RO454_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDY0936424.1"
                     /translation="MNQTMKFSGAVCPSVIPLDDSGQIDLTGLEKHMARLSDSGINGI
                     LLSGTIGEFATMSVRERAMLILEARKMSDLPLIAHISSTVEEDMLYLADAAYDSGYDA
                     VMVLPQYYYAQTSRQLLSYFRSLDRKLAGDWFIYNFPARTGCDVDAHLVRMLAESCPR
                     FIGIKDTVDCASHTRAIVNAVTPLRKDFAVFAGFDEYFVPNLMNGGAGVLSGLNNVVP
                     ELFAQIMRAYRAGDLNEVAGLHKEIGRLSGIYAIGDDFVSTIKTAISRKYGYMTPVSR
                     NHNGQLTADQADCLDKLFGL"
BASE COUNT         1316 a         1289 c         1282 g         1313 t
ORIGIN      
        1 cagcatggga taaaaaaaat ataaaaccgg gggattatta ctctgtgcag gctgtttcta
       61 tgtttacgac aaaccagttt attctccttt gtcctaagga tattataaag ttttatcggg
      121 aaagctggaa gttcgagttg cttaatgtag acttcgatat aaataccagg aagaaactcc
      181 aggaagaagc tgcatattgc gtaccaattt ctgcaatatc tgtaaaacga tgggatatgc
      241 ctcttcaggg ctcgaaaccc tcaagttaga aggcaactag ggcgactgta ttctgtgacg
      301 aaatgaatgg ctggctcact ataataatga gcgaacccat cagggaaaaa tgtgcaatgg
      361 gcggacgcca atggaaacgt tactcgatgg aaaacgcatc tgggccgaga aaaatttaag
      421 ccagatgtaa tctgacagat acctgtataa ataaccgata actgtcagat caggtctgag
      481 ctaatacatt tcaataaagg tacttgctcg cgctctgtca ttttctgaaa ctcttcatgc
      541 tgcatttcga ttttccttct aagatcataa ctcgcatacc tgttcttttt atgtatatct
      601 atatctattt ttgtgacgag ttcaaaccac aatgttcgcg ctccccatgc agcgttatga
      661 aacatttccg caacggctat agagtctgta ttaactgctt cagtcatgta gtcgctaata
      721 cgttcagcgg taattttcgt tatttcatcg taattcattt tatctcctac aagattattt
      781 aatggaatgc aaaatatctg gtctgataat aatgttccag aattaatatc tgcaggctat
      841 gttaagttgt ccccacacac aatctacctg ttttctcgtg tgtgccacag acgcaataca
      901 taaatagtct ggccattaat ttcgtaacgg atttcgtact cgccagcaaa aatccgtctg
      961 acctccctgg gttcaaactg aaacaactgt tctcccatac gtggatgagt taacagaatg
     1021 accggtgcct gtgtcaggga ctgtaccgtt ctggcggcag caggtttact ggccagcacc
     1081 agaaaatcaa ataaccgcga caaatcagaa agcgccttac tggtccactt cagttccatc
     1141 agcgtggcac cggtaacgga ttgtcagtac tgaggctgtc cgaccaggcc tgtacagcct
     1201 gatggtcgat aacctgtccg gatgtcacat cgtccagggc ttccagcgtc aggcgattac
     1261 gctcctcctc ctgggcaagc catgcagaaa gcgcctgttt gataacccag ccccgggaac
     1321 gttccagtct ggcggccatc tggtcgactt tatcagccat aggtaatggc acatgcgcgg
     1381 taattacttt tgttgcttgt gcggcattgt ttttcatttg catatccgat tagttgttaa
     1441 tcatatttaa tcatagtgat tactttcaga cggaacaagg caaataatgc gcgtcagcgc
     1501 atgtaaggcg gcatacggag gttacatgta gcacaggtgt aaaaccgccg aagcccggcg
     1561 taagccggta ctgattgata gatttcacct tacccttttc catcctgcta agccacaccc
     1621 gctttcagct atgagagagc ctttgtgcgc agtcggggcg tatctccgcg ttagcgggcc
     1681 gtgagggacg cttacgagcg tggatgagag acgtacagtg gtttcttcta gcttttattt
     1741 cctctcaggt cccgaattgt caacttctga atcgtggggg cccctttggg gtgacgattc
     1801 agtggttgac aactgcacat aaaacaccct gtgtgtgggg tgatggttca cgtagtggtc
     1861 catcgccctg ccaggggtca gtcgccacta caccatcact tgagaaagag aagggacttg
     1921 ctgttatgtc agtgaaactt tggtaaatta tcgtatcctg tggcaggatg atggtttgtt
     1981 acagcattgc aattcattaa aaagggggct tacgaagaac agtggatatg atcgcaacta
     2041 aaacgcctgc cattgataaa gctgcccgaa tatttcattt tattgccggg aaaggctctg
     2101 ccacctacag tgaaattcac cagaatgtcg gattgcctca aagcaccacc tcggccctgc
     2161 ttgccagcct gaccacgcat gcctttctga gacaaaaaaa tggccggtat tatctggggc
     2221 taatctttta cgagcttggt aataaagcca ttcaggattt tgattttcgt gaactggctg
     2281 aagagtcact cgtttttctc cgtgaccaga cccgtcttgc ctgccatctg ggcgttctgg
     2341 atggttccac cggcatctat ctggcgaaaa aagaaagcca gaatgcgatt atcgttcgca
     2401 gctggacggg aaaacgactt tccctgcaca gttcctccct tgggaaagtg ttgttggcct
     2461 ggcttccgga agaccacatt gatgctcttt tacccacaga acaccttcct gcgtttactg
     2521 cccggacaat tacaacacgt aaagcgttga aagctgagct ggaggtggtc cgcctgcagg
     2581 gatgggcgtt tgataatcag gaagatgtcg acgatgtcac ctgtattgcc gcgccggtat
     2641 ttaacagtga tcaggccgtt gttgccgcaa taagtatttc cggggttcgt tttcagatgc
     2701 ctgaggaaaa aatacctgag tatgcgaacg cggtgatgaa agccgctggc atgatgtctg
     2761 aaaagttatg cggtatatga ctggtccgct cacaggtcat gataacccgt gagcggcgtt
     2821 cggccttacg ttgctctgac gcgggaggct ccgtgttttg cccacaacgc agtgatcacc
     2881 ggcacgacaa tggcggtgat caccacacag gatgccacca gagtggtggc tgccggtgcg
     2941 gcctcagcga agtccggcgc gatctcggct accagcaacg gagtggccac ggcagcgcca
     3001 gcagtactcg atgcggcaat tccagccgtg ccacggccct tacccagcat gatatccatg
     3061 attatcagtg gtacgcctgt gataacaatg actgccagag ccataagaat acccagcagt
     3121 ccggtgtcga tgatgacgcc aagattaatg gtattcccca acgcaaatgc aaagaatggg
     3181 atcagacttt tggtggccct ggaaaacaat tcccgaagtt cgggatcaag attacccagc
     3241 aaaaacccca ccagaaaggg gatcaggacg ccggcgaggt gttcaggttc gaacgttgcc
     3301 tgcccggcgg accccagaat aatcatcgtc atcagggggc cggactcaag cgagataagt
     3361 acagaagcac cggcttcttc ttttgtgccg tactcctgca tcagggaagc ataaagcccg
     3421 gcattggtca tatccatcgc cgcaacaatc gccagaacag ataaaccggc aaagaaacct
     3481 gtttttacca ttcccccttc cggtaggagc tgggcaaaaa caaaggcaca gacccaggca
     3541 gtggcgattt ttgtggctac cagtgtgcca gactttttga gaacggtccc ggttgcactg
     3601 attttaatgg aggcgcccat acagaacagc cagacagcga ggatggggac cacgccggtg
     3661 atcatgccat tagtaaatga accaaagtat ttgccggtac ctggccagaa ggtatgcagt
     3721 accgccccca gaaacagggg ggccagcatc attcctcccg gaattttttc aactgttttt
     3781 ttgatattca ttatcgtccc tttttaatgt cagatacggc cagtcagagc accatgatgg
     3841 cttcaatttc aataacagcc ccctgcggca gggcggcgat acccaacgta gccctggcgg
     3901 ggtacggtgg cgaaaagtat tccgacataa tggcgttaaa tcgcgggaac atgttcacgt
     3961 cggtcagata ggcattcagc ttcacaatat ccctatcact ccctcctgcg gcctctgcca
     4021 ccgacagcaa atttttaaac gcctgacggg cctgttcatc gaaatctgtt gagaccactt
     4081 gcatggtatg cgggtcaaaa ccgacctgac cggcgagata aatggtttcc ccggctttca
     4141 ccgcctgaga taatgcccca ccgggggcag gagccccggt ggtataaatg atgtcttttt
     4201 tcatgatgtg cctcctgtcc ttttgcggtt acagaccgaa caatttgtct aggcagtctg
     4261 cctggtcggc tgttaactgg ccgttatgat tccgtgagac cggcgtcatg tagccatact
     4321 ttctggaaat ggcggttttg atcgtactga caaaatcatc accaatggca tatatcccgg
     4381 aaagtcgccc aatttctttg tgtaatccgg cgacttcatt gaggtcgccg gcccgatagg
     4441 cccgcataat ttgggcaaaa agctccggca ccacattatt cagtccggaa agaactccgg
     4501 cgcctccgtt catcagattg ggtacaaagt actcatcaaa accggcgaat acggcaaaat
     4561 cttttctcag cggtgtgacc gcgttgacga tggcccgggt gtgagacgca cagtccacag
     4621 tatctttgat gccaataaac cgcgggcagc tttccgccag cattctgaca aggtgagcat
     4681 ccacatcaca tccggttctg gccgggaagt tatagataaa ccagtcaccg gcaagtttac
     4741 ggtcaagact gcggaaatag ctaagtaact gacgggatgt ctgggcgtag taatactgtg
     4801 gcagtaccat tactgcgtca tagccactgt cataggcggc atcagccaga taaagcatgt
     4861 cctcttcaac ggtagatgaa atatgggcaa tcaaagggag gtcactcatt ttccgggctt
     4921 ccaggatgag cattgcccgt tcgcggacgc tcattgtggc aaattccccg atggtacctg
     4981 atagcagaat gccgttgatg ccggaatcag aaaggcgtgc catatgtttt tccagccccg
     5041 tgaggtctat ctgaccgctg tcatccagag gaatgacgga agggcagact gcccctgaaa
     5101 acttcattgt ctgattcata ttatttgttc cttttgcgtg gtatcagcgt catgcctgct
     5161 gatacccgcc ccggccttta cggaagaagg atggatatca
//
LOCUS       DAPQZD010000249         4963 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPQZD010000249 DAPQZD010000000
VERSION     DAPQZD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.252"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(1..234)
                     /locus_tag="RO454_004713"
                     /pseudo
     CDS             complement(1..234)
                     /locus_tag="RO454_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            223..630
                     /locus_tag="RO454_004714"
     CDS             223..630
                     /locus_tag="RO454_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDY0936425.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            768..1652
                     /locus_tag="RO454_004715"
     CDS             768..1652
                     /locus_tag="RO454_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDY0936426.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1684..2883)
                     /gene="tet(A)"
                     /locus_tag="RO454_004716"
     CDS             complement(1684..2883)
                     /gene="tet(A)"
                     /locus_tag="RO454_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDY0936427.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2989..3639
                     /gene="tetR(A)"
                     /locus_tag="RO454_004717"
     CDS             2989..3639
                     /gene="tetR(A)"
                     /locus_tag="RO454_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDY0936428.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3671..3913)
                     /locus_tag="RO454_004718"
     CDS             complement(3671..3913)
                     /locus_tag="RO454_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDY0936429.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3955..>4963)
                     /locus_tag="RO454_004719"
     CDS             complement(3955..>4963)
                     /locus_tag="RO454_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDY0936430.1"
                     /translation="ALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTGHVN
                     PKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTA
                     GFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAHW
                     DDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVY
                     LERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGK
                     P"
BASE COUNT          893 a         1547 c         1590 g          933 t
ORIGIN      
        1 gcggaactgc cgcgagccct tgacccagat gtcgccggag cgcagggcgt tcttcagctc
       61 ggacagggcg cagatttcgt agaatttccg gtcgaggcct tccggggtga tcaccagcgg
      121 cttccagcgc ggcttgatga aggccgtggg tgcatcggcc ggcaccttgc gcaggttgtc
      181 ggcgttcatc tcacgcaggg tctgcacggc tgccagcacg ccttgcgcgg ccggcccgtg
      241 ctgttcccca acttttcgct cggcgaagag tatgaacatg cgccgccggc gacgaatcgc
      301 caaatctcac cgtatctccc gagcggacga tttcgcaccg gtctgcccgt cgaagggctt
      361 gcgatcgaac ggggcgacct tttctatgca tgtccgcgag ccagcgtctt ttatggcacg
      421 gcgctcgacg ccgaccttcg gacgcgcggc gtaagcacgc ttgtcatggc cgggataagc
      481 accaccggcg ttgttctttc aagcgtcgcc tgggctagtg atgcggacta cgacgtgcgt
      541 ttggtccagg actgctgcta cgacccggat cgggatgccc acgaagctct gttgcgttcc
      601 gggttcggcg gacgtgtaca ggtcgtgtaa ttttcgggcc tcgcatgatc gcggccatag
      661 ccgcaggtgg attggtgcga ggcagggcca gtccaagtca gggtgcggtc atcgcggcgc
      721 ctgcaactct caaaaaccca tcgccgtagc ggaggtcttg taatcgcatg tcattacgca
      781 cgcccgattt attgttcaca gcgatagcac ctgccatttg gggcagcacc tacattgtca
      841 ccacccaata cctgccgaac ttctcaccga tgacggtcgc gatgctgcgg gcgttgccgg
      901 cgggtttatt gctcgtgatg atcgtccgac agattccaac gggaatctgg tggatgcgca
      961 tcttcatcct cggcgcactt aatatttcgc tattctggag cttgttgttt atttcggtct
     1021 accgcctgcc gggcggggtc gcggcgacgg taggcgctgt gcagccgctg atggtcgtgt
     1081 tcatctctgc cgctctgcta ggtagcccga tacgattgat ggcggtcctg ggggctattt
     1141 gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta gatcctgtcg
     1201 gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg ctgacccgca
     1261 agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg gccggaggac
     1321 ttctgctcgt tccagtagct ttagtgtttg atccgccaat cccgatgcct acaggaacca
     1381 atgttctcgg cctggcgtgg ctcggcctga tcggagcggg tttaacctac ttcctttggt
     1441 tccgggggat ctcgcgactc gaacctacag ttgtttcctt actgggcttt ctcagcccgg
     1501 ggaccgccgt gttgctagga tggttgttct tggatcagac gctgagtgcg cttcaaatca
     1561 tcggcgtcct gctcgtgatc gggagtatct ggctgggcca acgttccaac cgcactccta
     1621 gggcgcgtat agcttgccgg aagtcgcctt gacccgcatg gcataggcct atcgtttcca
     1681 cgatcagcga tcggctcgtt gccctgcgcc gctccaaagc ccgcgacgca gcgccggcag
     1741 gcagagcaag tagagggcag cgcctgcaat ccatgcccac ccgttccacg ttgttataga
     1801 agccgcatag atcgccgtga agaggagggg tccgacgatc gaggtcaggc tggtgagcgc
     1861 cgccagtgag ccttgcagct gcccctgacg ttcctcatcc acctgcctgg acaacattgc
     1921 ttgcagcgcc ggcattccga tgccacccga agcaagcagg accatgatcg ggaacgccat
     1981 ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg ccgtcggcaa tcattccgag
     2041 catgagtgcc cgcctttcgc cgagccgggc ggctacaggg ccggtgatca ttgcctgggc
     2101 gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg atcgtggtcg cgtcccagtg
     2161 aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc acctgtccga caagttgcat
     2221 gatgaagaag accgccatca gggcggcgac gacggtcatg ccccgggccc accggaacga
     2281 agcgagcggg ttgagagcct cccggcgtaa cggccggcgt tcgcctttgt gcgactccgg
     2341 caaaaggaaa cagcccgtca ggaaattgag gccgttcaag gctgccgcgg cgaagaacgg
     2401 agcgtggggg gagaaaccgc ccatcagccc accgagcaca ggtcccgcga ccatcccgaa
     2461 cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc tcatcgccat cagtgatatc
     2521 ggcaatataa gcgccggcta ccgccccagt cgccccggtg atgccggcca cgatccgccc
     2581 gatatagaga acccaaagga aaggcgccgt cgccatgatg gcgtagtcga cagcagcgcc
     2641 ggccagcgag acgagcaaga ccggccgccg cccgaaacga tccgacagcg cgcccagcac
     2701 aggtgcgcag gcaaattgca tcaacgcata cagcgccagc agaatgccat agtgggcggt
     2761 gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc
     2821 gatgccgaca gcgtcgagcg cgacagtgct cagaattacg atcaggggtc tgttgggttt
     2881 cacgtctggc ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac
     2941 tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg
     3001 cagccgaata cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt
     3061 ctgacgacac gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac
     3121 ttcaggaaca agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat
     3181 acgcattcgg tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc
     3241 agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga
     3301 ccgggcgcac cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt
     3361 ttttcggccg gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc
     3421 gtgcttgagg agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag
     3481 gctccgctct cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac
     3541 gcagcgttcg agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt
     3601 gtcaggaacg ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc
     3661 aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca
     3721 gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac
     3781 ggccgcgctc ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc cgctcgtggg
     3841 ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcggccg tcttcacgtt
     3901 ctgccttgcg cagatgagat aggggagccc gcagaattcg gaaaaaatcg tacgctaagg
     3961 ttttccgggc atccgtaagg gccgaaactt cccgtcttcc agtctgcggc tctgccgcca
     4021 gacgtaatcg ccggttaggt tgatgtgctc ccagcccagc ggcgacagga attgcagcag
     4081 ctcgccgtcc accggcttgc cggcctcgac caacccctgg gtggcgcgtt ccaggtacac
     4141 cgtgttccac agcacgatag ccgccgtcac caggttgagg ccgctggccc ggtagcgctg
     4201 ctgctcgaag ctccgatccc tgatttcccc aaggcggttg aagaacaccg ccctggccag
     4261 cgagttgcgc gcctcacctt tgttcaggcc ggcatgcacg cggcggcgca gttcaacact
     4321 ttgcagccag tccaggatga acagcgtgcg ctcgatccgg cccagctcgc gcagggccac
     4381 ggccagtccg ttctggcgcg ggtagctgcc gagcttgcgc agcatcagcg aggcggtgac
     4441 ggtgccctgc ttgatcgagc tggccaggcg caggatgtcg tcccagtggg cacgcacgtg
     4501 cttgatgttc agggtgccgc cgatcagcgg gcgcaacgtc gggtaggctt gcacgccctg
     4561 cggcacgtac agcttggttt cgccgaggtc gcggatgcgc ggcgcgaagc ggaagcctag
     4621 caggtgcatc agggcaaaga cgtgatcggt gaagccggcc gtgtcggtgt agtgctcctc
     4681 gatccgcagg tcggactcgt ggtacagcag gccgtcgagc acataggtgg aatcgcggac
     4741 gccgacattc accacgcggg tgctgaacgg cgcgtactgg tcggagatat gggtatagaa
     4801 cagccgtccc ggctcgctac cgtacttcgg gttgacgtgc ccggtgctct cgccccggcc
     4861 acccgcgcgg aagcgctggc catcggagga tgaggtcgtg ccgtcgcccc agtgggcggc
     4921 aaaggcgtgg cgatactggt ggttgaccag ctcggccaag gcc
//
LOCUS       DAPQZD010000250         4894 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPQZD010000250 DAPQZD010000000
VERSION     DAPQZD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.253"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(20..478)
                     /locus_tag="RO454_004720"
     CDS             complement(20..478)
                     /locus_tag="RO454_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDY0936431.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            598..1407
                     /gene="proC"
                     /locus_tag="RO454_004721"
     CDS             598..1407
                     /gene="proC"
                     /locus_tag="RO454_004721"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDY0936432.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1424..2539)
                     /gene="adrA"
                     /locus_tag="RO454_004722"
                     /gene_synonym="dgcC"
     CDS             complement(1424..2539)
                     /gene="adrA"
                     /locus_tag="RO454_004722"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDY0936433.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2641..2961)
                     /gene="psiF"
                     /locus_tag="RO454_004723"
     CDS             complement(2641..2961)
                     /gene="psiF"
                     /locus_tag="RO454_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDY0936434.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3080..4495)
                     /gene="phoA"
                     /locus_tag="RO454_004724"
     CDS             complement(3080..4495)
                     /gene="phoA"
                     /locus_tag="RO454_004724"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDY0936435.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4596..4856)
                     /gene="iraP"
                     /locus_tag="RO454_004725"
     CDS             complement(4596..4856)
                     /gene="iraP"
                     /locus_tag="RO454_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDY0936436.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT         1145 a         1333 c         1197 g         1219 t
ORIGIN      
        1 tgtaaataat aaattacatt tagccacgac tacgttgcac ttccagccac cacttctcca
       61 gctccgcagc aaaagcctgg cggtcacgtt gtgaaagact atctggtcct ccggtctgga
      121 tcccactggc acgtaaggta tccataaaat cgcgcatcgt caggcgctca cgaatggtcg
      181 ctggcgtata acgttcgccg cgcggattaa gcgccgccgc gcctttctca atggcttcag
      241 cagccaaagg tatatctgcg gtgatcacca aatcgcccgc ttcacactgc cgaacaattt
      301 cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt acgaataaat cgtgatggcg
      361 gcacgcgtaa actctggttt gctaccagta ccagcggcat ctgcatacgt tccgccgcgc
      421 gatacaaaat ctctttaatt acattgggac acgcgtcggc atccacccaa attgtcataa
      481 agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt ttgccttccg gcatagttct
      541 gtttatgctt ctgccagcga ttatcaaaac aatgaatttc acggcaggag tgaggcaatg
      601 gaaaagaaaa tcggttttat tggctgcggc aatatgggaa aagccattct cggcggtctg
      661 attgccagcg gtcaggtgct tccagggcaa atctgggtat ataccccctc cccggataaa
      721 gtcaccgccc tgcatgaaca gttcggcatc aacgccgcag aatcggcgca agaagtagcg
      781 caaatcgccg acatcatttt tgccgccgtt aaaccaggca tcatgattaa agtgcttagc
      841 gaaatcacct ccagcctgaa taaagactct ctggtcgttt ctattgctgc aggtatcacg
      901 ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa ttatccgcgc catgccgaac
      961 actcccgcgc tggttaatgc cgggatgacc tccgtaacgc caaacgcgct ggtaacccca
     1021 gaagataccg ctgatgtgct gaatattttc cgctgctttg gcgaagcgga agtgattgct
     1081 gagccgatga tccacccggt ggtcggtgtg agcggttctt cgccagccta cgtctttatg
     1141 tttatcgaag cgatggccga cgccgccgtg ctgggcggga tgccacgcgc ccaggcgtat
     1201 aaatttgccg ctcaggcggt aatgggctcc gcaaaaatgg tgctggaaac gggagaacat
     1261 ccgggaacac tgaaagatat ggtctgctca ccgggaggca ccaccattga ggcggtacgc
     1321 gtactggaag agaaaggctt ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa
     1381 aaatcagaaa aactcagcaa atcctgatgg cttttgccgg acgtcaggcc gccacttcgg
     1441 tgcggttacg tccggctttc tttgctttgt agagcgccaa atctgccgat ttcaaccact
     1501 cgcgatagtg actcatttgt ggattcagcg gcgcaacccc cacactaatc cgtaaagtta
     1561 cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc atgcacccgt aacatggcgg
     1621 tgatggcgct ctcagctggc gtaccggaca tgattactgc aaactcatcg ccgccaaacc
     1681 gaccaatcac atcgctaccg cgcagggtaa tttgtaactg tcgggtaagc gccacaatcg
     1741 cttcatcacc cacatcatgg ccccaggtat cgttgatgct cttgaaatgg tcgatatcga
     1801 taatcagtaa cgttgcatcg cgattatgcc gccgacagtt atcaaattca ttgcgtaaca
     1861 tagtttccca atgacgtctg ttatacacgc ccgtcatacc gtcgcgggta ctcatgacct
     1921 gcaacctgcg tttatgttcc gccagtttag tcgccgtctg gtagctgacc cagccaaaca
     1981 gcagaggata aatgacaata atgggaaggg agagccacca ttccagcggt gcgctattga
     2041 acgacacggt gatgcccgtc agttcgaggg tgacaaggca ggaaaccacc atcaacacca
     2101 gacccgcaac aaacagacgg gggccgcctg cccccatcaa attcagacac ataatcatca
     2161 acatcgcggt ggaaggcagc acgtttacgc ccattacgcc tacccacatt cccgctaata
     2221 ctgcatcggt ttttaagttg taaatttccc ggctaagcgg atcgacggcc ctgctcgcta
     2281 tctgccaggc taaatgcggc cagacgaacg cccagccgac caacaccagc caccaccagc
     2341 ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa gaacatgcca gccagaccaa
     2401 ccgcgcgggg tagtctgacg cgacgggcga agcgcagccc ggaccgctga tgttcgtttt
     2461 gaggagttaa aggaggttcc tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat
     2521 tcattatttt tgggaacatt cagaaacaat tttcccaaat tatagagacg ggactaaagg
     2581 cagaagtatg aaatttcggg tgagccattt tcgtagctca cccgttagca ctatcaggta
     2641 tcaggcggct ttcttgaggc aggcactcat aaacttatta cgatcatccc ctttcagaga
     2701 ttgttgtgtt gcttgattat tacattcgcg catcttttgc tgctgtggcg tcaaactttt
     2761 ttcaccaggc gcagatttgc tgttcttcag gcaatcactc atgtaggtct tacgagcatc
     2821 ccctttcaac gcctgcgccg tcgcctgctg attacaagag gtcatacgct gttgttgtgg
     2881 ggttaaggtt ctctcggcag cgccgacggt ggttaaaaaa accagaccga aaagcaaggt
     2941 aaccagtaat gtaattttca tcgcaccatc cctcttcatg ttttaaccat gagcgtatgc
     3001 gcccgtgatc tgtcattaag tctggttgct aacagcaaaa aaaccacccg gcagcgaaaa
     3061 ttcactgccg ggcgcggttt tatttcagcc ccagggcggc tttcatggtg tagaagagat
     3121 cggtctggtc ggtcagtcca acaacattgg cggcatgcgg gccatacgcc gcaatacgca
     3181 actgactgcc ggtatgttct tgtgaatcct cttcggagtt cccgtaactc atcaccatca
     3241 ctgcgccatc tttggtattt agcgcctggg tgaggcccgg agctttggta tctggcgcaa
     3301 ctatctggct ggcatgggcg tgatcagcgg tgactatgac caacgtgtta ccatcctttt
     3361 tagcgaattc cagcgcccgt tgtacggctt catcgagatc gaccgtctcg ccaatttgcc
     3421 cacaaggatt cgcagcgtga tcctgtttat cgattgacgc accttcaact tgcaggaaaa
     3481 agcctttctc atttttactc aacaattcaa tggctttgtc ggtcatctgc gccagggtcg
     3541 gtacactgtc attacgttgc ggattaggcg tacaggtgac tgcgggctta tctatattac
     3601 cgtggtacgt tgctttcggt ccttgccagc gcactggcat attgccgtca gcaaacagtc
     3661 ctaatagggg tttttgctga ttcgcttccg tcaccgaatt cagtgaggca gcatcgctca
     3721 ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt ttttccctgc cattcaccgg
     3781 cggttgccgt ttcagcaaag gtttttgcac cgccgccaag cgtaacatcg gcacgggcgt
     3841 taagcagctg ttcggtaatc gatccttttc cgcctttttc cagagcgtta cccggacatt
     3901 tttcactggt cgcacttgga ccgtagcatt tgcgcgaggt cacatgcgcc accagcgcag
     3961 cgggcgtggc atcctgcaac tctgcggtag aaacgttacc ggtcgccaga cctgcggctt
     4021 ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat atcgacgccc agcgcgccgt
     4081 tataggtttt gacaccagtt gaccaggcgg ttgctgatgc agccgagtcg gtgacgtagt
     4141 ccggtttgcc ggttttttta ttcagcgcat agtgagtgta ttgcccggta agcggtaagg
     4201 catcgatacc tttaaaaaag ccgcccgcac cttcggcata attgcgtgcg gcagtaattt
     4261 ccgagtcccc catcccatcg ccaatcagca aaataatatt ttttgcaggt ttatcgctaa
     4321 gagaatcacg cagagcagcg gtctgatcac ccgttaagcg gcgagcaccg ccgggtgcag
     4381 taatatcgcc ctgagctgcc cggttttcca gaacaggcat ttctggtgtc cgggcttttg
     4441 tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat agtgctttgt ttcactttat
     4501 tttctccatg tacaaataca ttaaaaaata aaaacaaagc gactataagt ctcggccgtg
     4561 acaactttat gacagctgtt gaaaagatta actttttact gacgaggatg cctcaataac
     4621 ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag gaatgctggc atcgggcttt
     4681 acctcgtaca gcgccccctc tacctgatca atcaaccgct gttgatcatt ttgcgccata
     4741 ttgcgaagca ttgccgtgac gataatctcc aaagcttcta cctgcgcaca cagttctttc
     4801 gactcttctt ctttttgggc aagcttaaat aacaactcag caatgagatt tttcatgtct
     4861 gtatttcctt atccaaagta tggagaagtt aaca
//
LOCUS       DAPQZD010000251         4891 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPQZD010000251 DAPQZD010000000
VERSION     DAPQZD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.254"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1764
                     /locus_tag="RO454_004726"
     CDS             <1..1764
                     /locus_tag="RO454_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HDY0936437.1"
                     /translation="LTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            1757..3367
                     /gene="tssA"
                     /locus_tag="RO454_004727"
     CDS             1757..3367
                     /gene="tssA"
                     /locus_tag="RO454_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDY0936438.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            3373..4791
                     /locus_tag="RO454_004728"
     CDS             3373..4791
                     /locus_tag="RO454_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDY0936439.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLKTSANEEDWVDIEVNKNNKTVNIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVDLTKNGVEFYWSRNGSRGGGIGESIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
BASE COUNT         1234 a         1205 c         1457 g          995 t
ORIGIN      
        1 ctgaccttct ggatggcgaa cctgccggaa cacccgcagt ggaaaacctc accgccaccg
       61 gaactgaccg gcgcggtgcg taaaatcctg ctgcgccaga ttggtgtgcg taatgccgaa
      121 aacaccctct accagaacgt gctgcaacag gtgtcccgca actacgccga tatgacgctg
      181 gcggacatga ccggggatac cctcactgaa tctcttttca gtacggaaca gacggtgccg
      241 gggatgttca cccgtcaggc atgggaagga caggtcaggg aagctatcga gcaggtggtg
      301 acggcgcggc gcgaggaaat cgactgggta ctcagcgacc gccagcagga tacctctgct
      361 gatatctcgc cggatacgct gcgtaaccgc ctcacctcac gctactttac cgactttgcc
      421 ggaagctggc tggcgtttct caacagcatt cgctggaaaa aggaagactc gctctccggc
      481 attctcgacc agctgacgct gatggccgat gcccgtcagt cgccactgat tgcactgaca
      541 gacactattg cgtggcaggt ggcaacaggc agggaaaatc gtggcctgtc agactcgctg
      601 gcgaaatcgg cacaggaact gtttaacggc aaggagaaaa cgccgcagca atcccgtgaa
      661 ggcgacgtgc ctgccgggcc gctggataaa accttcgcgc cgctgctgcg gttgctgggc
      721 gataagtcag gaggcggtgg tggtgatacg caactcaacc tgcaaacgta tctgacccgc
      781 gtcacccgcg tacgcctcaa actgcaacag gtgaccaacg cccccgaccc gcaggagatg
      841 acccagcaac tggcgcagac ggttttacag ggcaaaaccg ttgacctcac cgacacccgc
      901 gactacgggc ggttaatcgc cgccagtctg ggcgaagaat ggagtggctt cggtcaggcg
      961 ctgttcgtcc gcccggtgga acagtcgtgg cggcaggtgc tgacgcccgc agcggacagc
     1021 ctgaaccgcc agtggcaacg ggcgattgtc agtcactgga atcaggactt cgctggccgc
     1081 tatccgttca aagcctcaca gaacgatgcc tctcttccct tgctggcgca gtacctgcgc
     1141 gatgacggac gcatcaacca gtttatcgcc gccaaccttt ccggtgttct gaaacgtgaa
     1201 ggccgctact gggtggctga cgccatgaac acgcaggggc tgacggtcaa cccggacttt
     1261 atccgcgccc tgaaccgcct gcgcgatgtg gccgataccg cctttgccag cggcgatgcc
     1321 ggaatacatt tcgaactgcg ggcaaaaccg gcgcgtgacg tgatgaaaac gcatctggtg
     1381 attgacgggc aggaactgga gtatttcaac cagaaagaac gctggcagcg ttttaactgg
     1441 ccggatgaac agtggcaacc cggcgcatcg ctgagctgga ccagcacaca ggcgatggaa
     1501 cgcatactgg cggattaccg gggaagctgg agccttattc gcctgctgga acgggcgcag
     1561 gtaacaccgg tcgacagcag cacctttaaa gtggtgtgga aagcgcagga cggcctgcca
     1621 ctgaattacc tgctgcgggt tgaacagggt aaagggccgc tggcgctgct ggaactgaaa
     1681 aacttccgcc tgccgggaca ggtatttctg accggcagga gtatgaagga tgcggaagag
     1741 tatggggaag acgccgatga gtgaactgtt gcaaaagctg acccgtagct gttttgccga
     1801 ccgggatgca ctggatgtgg cgcgaacaca ggcggcgctg tggcagacat ggctgctgcc
     1861 ggtgacggca gacacgccgg tcggggaaga ccccggctac cacgacgatt tcctgcgtat
     1921 ccgcgacgaa atgaacaaac tgtccggtgc cgataccggc cttatctgcc agcttgccga
     1981 atccctgctg ctgacgcagg ccaaagacgt gcgcatcgcc acttactaca tctgggcgcg
     2041 actgcaccgc gacggtgagc gcggactggc tgaaggactg gcgctgttag ccgggctggt
     2101 ggaacgcttc ggtacgcaac tgctgccctc gcgaccggca agccgaaaaa tggcgctgga
     2161 gtggctggcg ggggaaaaga tgctcgacag ccttgcccgc tacccggagg tggcaaaaga
     2221 ggattttgcg aacattgttg ccgctcttaa tcaactgtcg gtttccttca ctgcatggcc
     2281 cgaagaccag cattccccct ccctgatgcc gctgattaac gcgctggaat cacgtctggc
     2341 gcagtcgggc gggatgaacg cggtggtgcc gcaaaacagc agcggcgttc cggcaccgtc
     2401 ttccccggtg gatgcgccgc aggtacagac catcacctcc gggcgcgacc tactcgacca
     2461 ggcgaaagtg ctggcccgtt atctgaacga acagccgcag ggctggcttt cagcacaccg
     2521 gctgatgaaa accctgcgct gggacacggt gcatgaactg ccgccggacg tggacggcaa
     2581 aacccggctg gccccgccgc gcaccgaatc acgcaaccag cttaagcggc tgtatgccca
     2641 gcagaactgg acggaactgc tggagcaggc cgacctgatg ttcagcaccg gtgtcagcca
     2701 tttctggctg gatatccagt ggtatctgca tcaggcgctg gcgaaggcgg gcgcaccgtg
     2761 ggacagatgg acagcggtta tccgtcagga cctggcgctg ctgctggagc gcctgccggg
     2821 gctggaaaat ctggcatgga acgacggcac gccgtttgcc gatgaggtga cgcgcaactg
     2881 gatagcgcag caggtcatga tgcgggaaga cggcgcatgg cttgcgggga aagccgctgt
     2941 gccgacagac gacgccacaa atgacgtact ggcgctggag ccggaagcac tggagatggc
     3001 tgacagtcag ggcgtggaag cggcgctggg ctggatacag acccgtccgg gcatcaccac
     3061 ggcacgacag cgcctgttat tgcgactgct gatggcacgg gtggcggaac agtacggtaa
     3121 aaacgagatg gcgctcctgc tgctggaaga actggatacc gccgcacagg gaataacgct
     3181 gacacagtgg gagccagagc tgctgtttga agtaaaagcc cgacaactga aactgctgcg
     3241 tctgcgggcg caccgctatg cggataaagc cctgctgaac cgaaaaatgg aaatcctgct
     3301 gggaacgctg gtggcaatcg atccggtacg ggcagcggta ttgtgtgata cgcaacataa
     3361 ggactgatgg caatgggaac aggctacttt ctggtcaggg gggataagac cacctgtggt
     3421 ggaaaaatta ttgaaggggc ggacgaccat acgatcatgg gtatcccgca ggcacgggat
     3481 atggaccggg tcacctgcgg cagatatccg ggaatgttta ttattgtggg cggcgttcct
     3541 gaaacagata ttcacggcag gctgatggcg gggacgctgg acagtcagag cagttgcccg
     3601 tgtaaagcac ggtttattgc gtcaatgatg gatgatacgt atgagacgga tgacggggaa
     3661 agtgaagcgg aacaacatgc gcagtcggcg aaaaaggatc tgacttctgg ttctgattca
     3721 tcctctgatg atgtaaaact ggattacaga attaaattat caggtaataa gatactgacg
     3781 ccactgaata tacctgatta taaagaaatg ataaacggtg gtagcacgaa gaatacagaa
     3841 aaaatagatt ttaccatcac aaataaaggc gatgaggctg aaggactttc cctggaagtg
     3901 cttgatggca atgaagtgat ttatagtgaa cggcagaccg gtaaatattt tactaaggga
     3961 gagcatgcct ggcaatggga tggctacagt aatcaaggca tactggatac aactaagcta
     4021 aaaagtaaat cactactcgt aagactgatt gctttatgtg gtgatatgat gataaaagtt
     4081 gattatccat tgaaaacttc tgcgaatgag gaagattggg tagatataga agttaacaaa
     4141 aacaataaga cagtaaatat aatatggcga ctggcagtta gtgatggagg gataaaagga
     4201 agtaatccta agttatcccc agttccttat aacgacttgg ttgacctgac caaaaatgga
     4261 gttgaatttt attggtctcg aaatggaagt cgcggcgggg ggattggtga aagtattgtt
     4321 acagcgattg gtgtttttaa agtaaatgta aaggctgaga taaatattac tccatccatg
     4381 cgaacttttt ctttgatatc atcattagat cctgactttc aggcatccgt tagtctatca
     4441 ggatttgaaa aaatatatta taactacggt gatagttata aggatattca agatgaatta
     4501 caggcacttc ttgacgctaa taacagatat aaatgggata gtgctcatga aatggggcat
     4561 aaagtactgg atgaatatgg agagggttct agccctgatt attcgtggac tcataaagga
     4621 acatctactc ttatgcaaaa aacaatacca gggaatgtta tgcccgctca gggagagatt
     4681 gatgtaatga aatatggaaa atatcgtcct gacatgtata ctcgattggt tgcagcagat
     4741 gaagatgttc aggggttgat ctggttaagc aggataaaat ttgatgacta gagtagttat
     4801 tgctttatta tttcttgccg gatgtacttc gcaaatgaag tattccaatg tcgtcaggat
     4861 taggaatgta tgttcctcag atattaatgt g
//
LOCUS       DAPQZD010000252         4808 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPQZD010000252 DAPQZD010000000
VERSION     DAPQZD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.255"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            104..571
                     /locus_tag="RO454_004729"
     CDS             104..571
                     /locus_tag="RO454_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936440.1"
                     /translation="MLKTNSLRESMLHGCRWCQANPEKFTIFVESGNIETTGEAPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            564..1199
                     /locus_tag="RO454_004730"
     CDS             564..1199
                     /locus_tag="RO454_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDY0936441.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTEWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1211..1777
                     /locus_tag="RO454_004731"
     CDS             1211..1777
                     /locus_tag="RO454_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDY0936442.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKEGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            1795..2124
                     /locus_tag="RO454_004732"
     CDS             1795..2124
                     /locus_tag="RO454_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDY0936443.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            2128..3024
                     /locus_tag="RO454_004733"
     CDS             2128..3024
                     /locus_tag="RO454_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDY0936444.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSASIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3017..3547
                     /locus_tag="RO454_004734"
     CDS             3017..3547
                     /locus_tag="RO454_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDY0936445.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGARITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            3550..>4808
                     /locus_tag="RO454_004735"
     CDS             3550..>4808
                     /locus_tag="RO454_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDY0936446.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVVLTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLTTAAGKF
                     PYFTGNDVASLATLTKVGRDILAKSTVA"
BASE COUNT         1178 a         1334 c         1383 g          913 t
ORIGIN      
        1 taactgatat cactaaaaat gccgcgtacg gcgtcgatat cggcagcctg gtgctggaaa
       61 tcaataatcc cgcagcataa ggaagagcag gagagcaaaa cagatgctta agacaaacag
      121 tctgcgagag tccatgcttc atgggtgtcg gtggtgccag gctaatcccg agaaattcac
      181 cattttcgtg gagagcggca acattgaaac gaccggagaa gcgccctcgt ttgtttaccg
      241 ctatcagatg gtgatgtttg tcatggatta cgccggagag ctggacgacc tcacgctgcc
      301 gctgctggcg tggttatccg aaaatcagcc acagttgttg ctcaatccgg agcgtaatca
      361 ggacatcaaa ttttctgccg ttatcaatga cgatgacagc gccgatctcc tgtttacgct
      421 ccccctgcgg gaacgcgttc gcatcacgcg caacagtcag ggcacaccgc aggcagaaca
      481 cctgccggag ccaaaacccc gtctgccttc ttccgaaggc gactggtcgc atgtattcca
      541 ggatgtgacg tggggtgaaa gcgatggata aggcattcac ccgcgtggat gaaacctttg
      601 aggctatccg cgacagcctg aatcagcagg ccatcaataa catcgccaga aagctggcac
      661 aggatttacg tcgcgcccag caggcacgta tccggtcaca aaaagcgccg gacgggaccg
      721 aatggacacc acgcagacgc cgcgtaaccc ggatacagga gcgcattcgc tttatctgga
      781 ataacgaagc acgcacgctg aaaaactggc atcacgacac agggaaatac gggcgaacca
      841 ttaccgggtg ggatgaggat aaaaacaata tccgcacgtt ttaccgggat gacatcgacc
      901 gttttctgga aatacgcacc cggcgcatca accaggacag cacaaagcgc gtccccatgt
      961 tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc agatgcctcc ggtgtgacgg
     1021 tgggttacag cggcgtggcc gcacgtattg cacgcgttca tcagttcggt gagcgcgatc
     1081 aggttgcgcc gggcattttc accgattacc cggtacgtga gctgttgggt atcagccagg
     1141 cagatgagcg cctgatttat aacacggtgc tgggccggat tgcggaggct gtacggtgag
     1201 cgcagaactc atgcgactgc tgagcaacat catccgcacc gggatcatct ctgaagttga
     1261 tgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg gaaacaggct ggttgcgctg
     1321 gaacaccacg cgcgcgggag ccttcaatgt gtggctgccg ccatcaccag gcgaacaggt
     1381 ggtaattgcc tgcattggcg gcaacccgga aaccgccatg ataattggca gcctgtggag
     1441 tgatgccagt ccggcccccg gcaaaagcct gaaagaaatc gtggtcagcg cgccggatgg
     1501 cgcggtgttc cgctacgacg cagacgctgg cgcactgagc gccagcggca tgaaaacagc
     1561 caccctgcag gcatccgtca gcgtgacact ggatacgccc gtcgtggaat gcacaaacct
     1621 tctgagaacg gcgacgcttg acgtcacaaa agagggaaag atgagcggca atatcacgca
     1681 cagcggcggc aatttcacct caaacggcat taccgtgcat acgcataagc acggtggcgt
     1741 taaaggtggc agcgattcga caggaggccc gcagtgacaa cccgctacac aggaatgaac
     1801 ccggacggga cgggaaacct gaacgatatg gagcacctga aacagtcagt cagggacatc
     1861 ctgatcaccc cgctggcaag ccgggttatg cgacgggaat atggcagcct tgtgcctgat
     1921 ttgattgacg aacccatgaa taacaccacg cgtctgcaat gcatgagtgc tgccgtgatt
     1981 gcgctgacac gatgggaacc ccgcattgcc ctggacgcca tcgacgttgt ctggaaggca
     2041 ggaggccgcg ccggggtgac gctgtcgggc actgtcatgc agaccatgca gaatgttgaa
     2101 ttaaccatca cgctgagaga gtaaatcatg cctgctgttg atctttccca gttaccggaa
     2161 cccgccatca tcgcggagcc tgattttgag gcaattctgg ctgacacaaa ggccatgatg
     2221 attgcgtcct atcctgccga acagcgtgaa gccgtctccg ccgcgctgga gctggaatca
     2281 gaacccctga acgttatcgc tcaaaccatg tcgtttcgtg aaatgctgtt acgccagcgg
     2341 gttaacgagg gcgcacgcgc ctgcatgtta agccacggtt cagggacaaa cctggacaac
     2401 ctcgcgggca atatgaacac aaagcgcctg gttatcactc cggcaacgga taccaccgac
     2461 gcggtgatgg agagcgacac ctcgctgaga ctgcgggcgc agcgggcgta tgacggcctg
     2521 agtgttgctg gcccgtcagg tgcatacgag tattttgcac gcagcgccag cggtctggtg
     2581 cgtgatgcgc gggccatcag cccgtctccg gcctgtgtga cggtttccat tctgtccact
     2641 gaaggcgacg gcacagcaac ggaggcgttg cttaataccg ttcgcgccgt tctgaatgca
     2701 gaggataccc gcccggtggc cgaccgactg accgtacaga gcgccagcat cgtgacatgg
     2761 cggctgaatg caaaactgta cttttacccc ggcccggaat ccgaacctat tctggctgcg
     2821 gctgaatcgt cgttcaggaa gtggctggct gagcaggggc ttattggtca ggacgtggcg
     2881 ttgtcagcca ttgctgccgc acttcatgtg cacggcgtgc aacgcgtgga gataatcgaa
     2941 cccacacaga atatggccat cagcgacata caggcggcgc gctgtgagtc attcaccatc
     3001 agcgaaggtg ggcgtaatga gtaattcact gttaccgcca tcagccagca atttcatgcg
     3061 ttgcgccgaa gctgtcggag cgcgcattac agacatcccg gtagacctca acacgctgtg
     3121 gtcaccggac acctgcccgg tgcatctgct gccttatctc gcctgggcat tttccgttga
     3181 ccgctgggat cgcaactggc cggaagagac aaagcgacag gtgattcgtg atgcatggct
     3241 gatacaccga cacaaaggga ccatcagcgc actgcgccgg gccattgagc cgctgggata
     3301 cctcattcgc gtgtctgagt ggtgggagtt cggcggagaa ccgggaacat ttaccgttga
     3361 agtcggcacg ctggacagtg gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat
     3421 tgctgatgcc cgcccggtca gccgccacat gacagggctg aatatcattc aggagattca
     3481 gggagatatt ttcgcggcgg cagcaactta cgacggcgaa gtcattacca tttatccgga
     3541 cgattaagca tgagtaccac aacacgtaaa tttaaaaccg ttatcaccga tacgggtgcc
     3601 aaaaaattag ctcaggcagc cgcgccagat ggtaagcctg tccgcctgac tcatatggcc
     3661 gtgggcgacg gtggcggcac gttgcccaca ccagacagta agcagacccg tctggtgcat
     3721 gaggtgtggc gacatactgt taatcgcgtc atcctggacg caacacatca gaaccgcatt
     3781 attgcggagc tggttattcc tcctgaaacg ggcggattct ggatccggga aattggtgta
     3841 tttgatgagc acggcgattt aatcgcggtg ggcaatactg ccgaaagtta caagccagcc
     3901 gttgccgaag ggtccggtcg tgcacaaaca tttcgcacca ttctgaccgt atccagcact
     3961 gccaccgtgg tgcttaccgt ggataacacc atggtgatgg ccacagtgga ttacgtggat
     4021 gacaaactga aagagcatga acagtcacga cgtcacccgg acgcctcgct gaccgcaaaa
     4081 ggctttgttc aactcagtag cgccactaac agcgtgtctg aaacgcaggc tgcaacgccg
     4141 aaagcagtaa aggccgcgta tgaccttgct aacggtaaat atactgcgca ggatgccagc
     4201 acgacgcgaa aaggccttgt ccagctcagt agcgccacca acagcacgtc tgaaacgcag
     4261 gctgcaacgc cgaaagcagt aaaggccgcg tatgaccttg ctaacgcaaa atataccgct
     4321 caggacgcca cgacggcaca aaaagggata gtccagctca gtagtgccac caacagcacg
     4381 tctgaaacac tggccgcgac atcgaaagct gttaaggcgg taatggatga aacgaacaag
     4441 aaagcgccct taaatagtcc tgcactgacc ggaacgccaa caacaccaac tgcgcgacag
     4501 ggaacgaata atacccaaat cgcaagcacg gctttcgtta tggctgcgat tgccgccctt
     4561 gtagattcgt cacctgatgc actgaacacg ctgaacgagc tggctgcggc gctgggcaac
     4621 gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc gaaagatgcc
     4681 accctgacgg cgctggccgg acttactact gcggcaggca agtttccgta ttttacgggg
     4741 aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct tgcgaaatcg
     4801 accgttgc
//
LOCUS       DAPQZD010000253         4789 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPQZD010000253 DAPQZD010000000
VERSION     DAPQZD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.256"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..445
                     /gene="wcaM"
                     /locus_tag="RO454_004736"
     CDS             <1..445
                     /gene="wcaM"
                     /locus_tag="RO454_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="WcaM from Salmonella enterica is essential for
                     biofilm formation on HEp-2 cells and chicken intestinal
                     tissue but not on a plastic surface; may be involved in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDY0936447.1"
                     /translation="IGYGVIKGKYLSIPQNFRVNDIQLDNTHLAYKLRGIQISAGNAV
                     SFVALTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAK
                     KETLLSLANVHAVNEKGQSSVDIDRINHHIVNVEKINFRLPERRE"
     gene            620..1513
                     /gene="galF"
                     /locus_tag="RO454_004737"
     CDS             620..1513
                     /gene="galF"
                     /locus_tag="RO454_004737"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDY0936448.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDNASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            1886..2971
                     /gene="rfbB"
                     /locus_tag="RO454_004738"
     CDS             1886..2971
                     /gene="rfbB"
                     /locus_tag="RO454_004738"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDY0936449.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYFFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNSLEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2971..3870
                     /gene="rfbD"
                     /locus_tag="RO454_004739"
     CDS             2971..3870
                     /gene="rfbD"
                     /locus_tag="RO454_004739"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDY0936450.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3928..>4789
                     /gene="rfbA"
                     /locus_tag="RO454_004740"
     CDS             3928..>4789
                     /gene="rfbA"
                     /locus_tag="RO454_004740"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDY0936451.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLK"
BASE COUNT         1349 a          970 c         1188 g         1282 t
ORIGIN      
        1 aatcggctat ggggtaatta aaggcaaata tctctcgata ccgcagaatt tccgagtgaa
       61 tgatattcaa ttggataata ctcatcttgc ttataaattg cgcggcatcc aaatctccgc
      121 cgggaatgct gtctcctttg tggcgctgac taacattgag atgaagcgtg cgtcgctgga
      181 gttacacaac aaaccgcaac atctttttat gcgtaatatc aatgtgatgc aggaatcctc
      241 agttggacca gcattgagca tgaactttga catgcgcaaa gacgttcgag gcgtgtttat
      301 ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg aaaaagggca
      361 aagctccgtc gatatcgaca ggattaatca ccatattgtt aatgtggaaa agattaactt
      421 tagattgccg gaacggagag agtagatttg cgaccattcc tggaaaaatg gagtcatact
      481 taggaacaat gctactgcaa tccacaacga agcggcgtaa catcacaagt aattcagtaa
      541 tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat
      601 tttacaggtg gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtctagg
      661 gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa
      721 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
      781 ggtaactcat gcgtccaaga acgcggtcga aaaccacttc gatacatctt atgaattaga
      841 atctctcctt gaactgcgcg tgaagcgtca actgctggcg gaagtacagt ccatctgtcc
      901 gccgggcgtg accattatga acgtgcgtca gggcgaacct ttaggtttag gccactccat
      961 tttgtgtgcg cgacctgcca ttggtgacaa cccatttgtc gtggtactgc cagacgttgt
     1021 gatcgacaat gccagcgccg acccgctacg ttacaacctt gctgccatga ttgcacgttt
     1081 caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata
     1141 ctccgtcatt cagactaaag agccgctgga tcgtgaaggt aaagtcagcc gcattgttga
     1201 atttatcgaa aaaccggatc agccgcagac gctggactca gacatcatgg ccgtaggtcg
     1261 ctatgtgctt tctgccgata tttggccgga actggaacgt actcagcctg gtgcatgggg
     1321 acgtattcag ctgactgatg ccattgctga gttggcaaaa aaacaagcag ttgacgcaat
     1381 gctgatgact ggggacagtt acgactgcgg aaagaaaatg ggttatatgc aagcgtttgt
     1441 gaagtatggg ctgcgtaacc tgaaggaagg ggcgaagttc cgtaaaggta ttgagaagct
     1501 gttaagcgag taacaaattc ctgactggaa gtgtcggtca gtaagtaatt aaaaacggca
     1561 gtgaagattc atagcataag ttttgctcat tgaatctcac tgccgttgtc ttttttataa
     1621 gtacaataaa aacagcatgt tagcttgcaa tcatagttca aatttttcca ggattttcct
     1681 tgtttctaca gctgtttggt aagacaatta gcgtttgaat ttttcggatt tggcacgagt
     1741 gggttacgct cgtcacatcg tggaaatgta cgcagtgctc tggtagcggt taagccaggg
     1801 gcggtagcgt gcattaatgc atctattaat caaatctgga gcagtctatt tcacagcatg
     1861 ctctctggct atatggaata aaaaagtgaa gatacttgtt actggtggcg caggatttat
     1921 tggttctgct gttgttcgtc acataataaa taatacgcaa gatagtgttg ttaatgtcga
     1981 taaattaaca tacgccggaa acctggaatc acttgcagat gtttctgatt ctgaacgcta
     2041 tttctttgaa catgcggata tttgtgatgc agctgcaatg gcacggattt ttgctcagca
     2101 tcagccggat gcagtgatgc acctggcagc tgaaagccat gttgaccgtt caattacagg
     2161 ccctgcggca tttattgaaa ccaatattgt gggtacttat gtccttttag aagcggctcg
     2221 gaattattgg tctggtctgg atgatgaaaa gaaaaaaaac ttccgttttc atcatatttc
     2281 tactgatgag gtgtatggtg acttacccca tccggatgaa gtaaatagca atgaaacgtt
     2341 gccgctattt acggaaacga cagcatacgc gccaagtagt ccatattctg cttctaaagc
     2401 ttccagcgat catttggttc gcgcatggaa acgtacttat ggtttaccga ccattgtgac
     2461 taattgctcg aacaactatg gtccttatca tttcccggaa aagcttattc cactggttat
     2521 tcttaattca ctggaaggta aggcattacc tatttatggc aaaggagatc agatccgcga
     2581 ctggttgtat gtagaggatc atgctcgagc gttatatacc gtcgtaaccg aaggtaaagc
     2641 gggcgaaact tataacattg gtggacacaa cgaaaagaaa aacatcgacg tagtgttcac
     2701 tatttgtgat ttgttggatg agatagtccc gaaagagaaa tcttaccgcg agcaaattac
     2761 ttatgttacc gatcgtccgg gacacgatcg ccgttatgcg attgatgctg agaagattgg
     2821 tcgcgaattg ggatggaaac cacaggaaac gtttgagagt gggattcgta aaacggtgga
     2881 atggtacctg tccaatacaa aatgggttga taatgtgaaa agtggtgcct atcaatcgtg
     2941 gattgaacag aactatgagg gccgccagta atgaatatcc tcctttttgg caaaacaggg
     3001 caggtaggtt gggaactaca gcgtgctctg gcacctctgg gtaatttgat tgctcttgat
     3061 gttcactcca ctgattactg tggtgatttt agtaatcctg aaggtgtagc tgaaaccgta
     3121 agaagcattc ggcctgatat tattgtcaac gcagccgctc acaccgcagt agacaaagca
     3181 gaatcagaac cgaagtttgc acaattactg aacgcgacga gtgtcgaagc gatcgcgaaa
     3241 gcagccaatg aagtcggcgc ctgggttatt cactactcta ctgactacgt atttccgggg
     3301 accggtgaaa taccatggca ggaggaggat gcaaccgcac cgctaaatgt ttacggtgaa
     3361 accaagttag cgggagaaaa agcattacaa gagcattgtg cgaagcacct tattttccgg
     3421 accagctggg tctatgcagg taaaggaaat aacttcgcca aaacaatgtt gcgtctggca
     3481 aaagagcgtg aagaattagc cgttattaat gatcagtttg gtgcgccaac tggcgcagag
     3541 ttactggctg attgtacggc acatgctatt cgtgtggcac tgaataaacc ggaagtcgca
     3601 ggcttgtacc atctggtagc tagtggtacc acaacgtggc acgattatgc tgcgctggtt
     3661 tttgaagagg cgcgcaaagc aggcattccc cttgcactca acaagctcaa cgcagtacca
     3721 acaacagcct atcctacacc agctcgtcgt ccacataact ctcgccttaa tacagaaaaa
     3781 tttcagcaga actttgcgct tgtcttgcct gactggcagg ttggcgtgaa acgaatgctt
     3841 aacgaattat ttacgactac agcaatttaa tagtttttgc atcttgttcg taatggtgga
     3901 gcaagatgta ttaaaaggaa tgatgaaatg aaaacgcgta aaggtattat tttggcgggt
     3961 ggttctggta ctcgtcttta tcctgtgacg atggccgtca gtaaacagct gttaccgatt
     4021 tatgataaac cgatgatcta ttacccgctc tctacactga tgttagcggg tattcgcgat
     4081 attctgatta tcagtacacc acaggatact cctcgttttc aacaactgct gggtgacggg
     4141 agccagtggg gcctgaatct tcagtacaaa gtgcaaccga gtccggatgg tcttgcgcag
     4201 gcgtttatta tcggtgaaga gtttattggt ggtgatgatt gtgctttggt acttggtgat
     4261 aatatcttct acggccacga cctgccgaag ttaatggacg tagctgttaa caaagaaagt
     4321 ggtgcaacgg tatttgccta tcacgttaat gatcctgaac gttatggtgt cgtggagttt
     4381 gataataacg gtactgcaat tagcctggaa gaaaaaccgc tggaaccaaa aagtaactat
     4441 gcggttactg ggctttattt ctatgacaat gacgttgtgg aaatggcgaa aaaccttaag
     4501 ccttctgccc gaggtgaact ggaaattacc gatattaacc gtatttatat ggaacaagga
     4561 cgtttgtctg tcgctatgat ggggcgtggc tatgcatggc tggatacagg gacgcatcaa
     4621 agtcttattg aagcaagcaa cttcattgcc accattgaag agcgccaggg actaaaggtt
     4681 tcctgtccgg aagaaattgc ttatcgtaaa gggtttattg atgctgagca ggtaaaagta
     4741 ttagccgaac cgttgaagaa aaatgcttat ggtcagtatc tgctcaaaa
//
LOCUS       DAPQZD010000254         4756 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPQZD010000254 DAPQZD010000000
VERSION     DAPQZD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.257"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            107..1081
                     /gene="hemB"
                     /locus_tag="RO454_004741"
     CDS             107..1081
                     /gene="hemB"
                     /locus_tag="RO454_004741"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDY0936452.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1121..1972)
                     /gene="tauD"
                     /locus_tag="RO454_004742"
     CDS             complement(1121..1972)
                     /gene="tauD"
                     /locus_tag="RO454_004742"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDY0936453.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1969..2796)
                     /gene="tauC"
                     /locus_tag="RO454_004743"
     CDS             complement(1969..2796)
                     /gene="tauC"
                     /locus_tag="RO454_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDY0936454.1"
                     /translation="MSVLINEILHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2793..3560)
                     /gene="tauB"
                     /locus_tag="RO454_004744"
     CDS             complement(2793..3560)
                     /gene="tauB"
                     /locus_tag="RO454_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDY0936455.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3573..4535)
                     /gene="tauA"
                     /locus_tag="RO454_004745"
     CDS             complement(3573..4535)
                     /gene="tauA"
                     /locus_tag="RO454_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDY0936456.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1086 a         1381 c         1220 g         1069 t
ORIGIN      
        1 ctctttttca ttctcttctt gtcaacatcg cgacaacttt cgtaaaacac acccagcctg
       61 cttcaggtat actatgcccc tcgattccac aaacatcagg cagaccatga cagacttaat
      121 ccaacgccct cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg aagagacaac
      181 acttagcctt aacgacctgg tgttgccgat ctttgttgaa gaagaaattg acgactataa
      241 agccgttgaa gccatgccag gcgtgatgcg cattccagag aaacatctgg cacgcgaaat
      301 tgaacgcatc gccaacgccg gtattcgttc cgtgatgact ttcggcatct ctcaccatac
      361 cgatgaaacc ggcagcgatg cctggcggga agatggactg gtggcgcgta tgtcgcgcat
      421 ctgcaagcag accgtgccag aaatgatcgt tatgtcagac acctgcttct gtgaatacac
      481 atctcacggt cactgcggtg ttctgtgcga gcatggcgtc gacaacgacg cgactctgga
      541 aaatttaggc aagcaagccg tggttgcagc tgctgcaggc gcagacttca tcgccccttc
      601 tgccgcgatg gatggtcagg tacaggcgat tcgccaggcg ctggacgctg cgggctttaa
      661 agatacggcg attatgtcgt attcgaccaa gttcgcctcc tccttttatg gcccgttccg
      721 tgaagctgcc ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga acccaatgaa
      781 ccgtcgtgag gcgattcgtg agtcactgct ggatgaagcc cagggcgcag actgcctgat
      841 ggttaaacct gccggagcat acctcgacat cgtgcgtgag ctgcgtgaac gtactgaatt
      901 gccgattggc gcgtatcagg tgagcggtga gtacgcgatg attaagttcg ccgcgctggc
      961 gggtgctata gatgaagaga aagtcgtgct cgaaagctta ggttcgatta agcgtgcggg
     1021 tgcggatctg attttcagct actttgcgct ggatttggct gagaagaaga ttctgcgtta
     1081 atgatcatta tgccgggtta tccaacccgg catttccccc ttaccccgcc cgataaaacg
     1141 gaatatcccc aagaatcgtc gcccgatgca taatccgccg ctgtggcagg taatccgcat
     1201 tggcatagtg ctgagtcact cggttatccc aaatcgcaat atcattcggt tgccagcgcc
     1261 agcgcacctg aaactccggt ttagtgatat gggcaaacaa aaaacctaac aaggcttcgc
     1321 tctctttctc gctgacatca acaattcgtg tggtaaaacc ttcgttcaca aacagtgcct
     1381 gtttaccggt caccggatgc gttcgcacca ccggatgtag caacggtgga tttttcgcga
     1441 ccgcctcacg ccagcgttga tgctcagcct cggttttgcg gtacttgtat tccgggaacg
     1501 atttacggaa atcatgctcc gcacgcaacc cgctcaacag ctggcggaag ggaacagaga
     1561 gcgcctcata tgcggcaata ccactggccc agagcgtatc gccgccggtc gagggtaact
     1621 ctttcgccgc cagaatcgcc cctgcgggtg gcgtttgaat aaatgtcaca tcggtgtgcc
     1681 agttgtcgtt atccggcgga ttatcattat gggtatccag gacgatgatc tcgtcaaccc
     1741 cttcagcatg agggtaaaca gggtgaatat gcagctcgcc aaaacgctgg gccagcgcgc
     1801 gttgctgctg cggcgtgata gcttgatcgc gcagaaacac cacctgatgg cgcagcaccg
     1861 catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgttaagtcg gcccccgtaa
     1921 tttgtgcgcc aatatacggc cccagcgggg taatgctcag acgttcactc attgtacttc
     1981 tccatgccag ggcgtcaggc ggcgctgtaa cgcgcgcaga cccagttcta aaagaaaggc
     2041 gataatcgca atcaccgcga tccccgccag caccacgtca gtcgcgagaa attcacccgc
     2101 tgactgaacc ataaatccta aaccgcgcgt tgcggcaatc agctccgccg ccaccagcgt
     2161 agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca gcgcgccggg
     2221 caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg cggcacgaat
     2281 gcgaacctgc tgcgcgcttt tcacccccgc cagcgccgac atcgccaccg gcgcaaaaat
     2341 cgctaaatag atcagcaaga tcttcgaggt ttcgccaata ccaaaccaga tcaccatcag
     2401 cggcaaataa gccagcggcg gcaccggacg ataaagctcg attatcggat ccagaatgcc
     2461 gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca acaccgccgc
     2521 gagtagcgcc agcaaaatgc gcgtcaggct ggctgccaga tgctgccaca gcgtggcgtc
     2581 cataaatccc tgcggcccgg caatggtgag cagttttgcc agtacctgtt gcggtggcgg
     2641 caaaaacagc gggctaatca gttgcagcgc cgcgactgcc caccatacag tgagcaaaac
     2701 cgctaacgta ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
     2761 atgcgaatgc agtatttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
     2821 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
     2881 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
     2941 cctgggccgg gtgaaagcaa aaccagttca gtcgccataa acaccgcttc ttctatatcg
     3001 tgggtaatca acagcacctg ctttcccgtc tcctgccaaa gtttcagcag cagggtttgc
     3061 atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa taacagctgg
     3121 ggatttgccg ccaacgcgcg ggcaatcccc acccgctgac gttgaccacc ggaaagctgc
     3181 cagatgtagc gtttttctgc gccttccagc ccgacttttt tcaccatctg gtgtgcgatt
     3241 tccagtcgct gcattttctc tacacctgcc agttgcaggc cgaacgccac gttgtcctgt
     3301 acattgcgcc acggtaacaa cccttcattc tgaaaaacta cgccacgctc tgctcccggt
     3361 ccctgaatac ccttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccagca
     3421 atcaaattca gcaacgtagt tttgccgcag ccagacggcc ccagaactac cagaagctcg
     3481 ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata atcggcgtaa
     3541 agatgagaaa tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga ggtaacgtac
     3601 tggctgtaat cattcgctac agccgggact ttgccctgct ctttcaaaaa ctgcgcggtg
     3661 tcgatgatcg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
     3721 taggtattcc ccttcaccag ccccggaacg tcaccttccg gcacgccgct taaccgcgcc
     3781 agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
     3841 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
     3901 tctttccgca cgacccagac gtccagtgtc ggcgcgcccc actgcccgac ctgttcagaa
     3961 tcggtcagca ccttgccatc tttttccagg gcgttaaccg ccggtgccca tacataagca
     4021 ccatcaatat ctccccgctg ccatgcggcg ataattgcgg gcggctgcag gttcacaatc
     4081 tcaacttgcc caggtttaat accccagtgt ttcagcgccg ccagcaggct gtagtgggtg
     4141 gtggagataa acggtacggc gatgcgttta ccaatcaagt cttccggttt actgatagtt
     4201 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa gacttcaatc
     4261 ggcacctgtt ggctggttgc aaccgctaac gggctggaac cgaggttgcc gatttgcaca
     4321 tcgcccgaag ccagcgcccg cacgatgctg gctccactgt caaacttacg ccagtccacg
     4381 gttgctccgc tttctttagc aaaggtgttg tcggcctgag ccactttcgc cggttcggcg
     4441 gaggtttgat acgccacggt gacgttcacc gcttgcgcct gaaaagcgat gaatgccagt
     4501 gcggcaagaa atgtgttacg cgatgaaatt gccatgattg tctgctcccc cgtcttgtta
     4561 tgggagcagt atttcgttgc gggtaagttt gataaaggaa ttaaaacgtc ttgttaataa
     4621 caaagcgttt ttagaaaaaa atcggcaatg gttagcgagt ttgcgcattt tgctactttt
     4681 aacaaacatc gcctgatgtg acgctcacgc ctcttatcat gcctacaaat actggggccg
     4741 gataaatttt attctc
//
LOCUS       DAPQZD010000255         4717 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPQZD010000255 DAPQZD010000000
VERSION     DAPQZD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.258"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            153..869
                     /gene="nanC"
                     /locus_tag="RO454_004746"
     CDS             153..869
                     /gene="nanC"
                     /locus_tag="RO454_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDY0936457.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            889..1995
                     /gene="nanM"
                     /locus_tag="RO454_004747"
     CDS             889..1995
                     /gene="nanM"
                     /locus_tag="RO454_004747"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDY0936458.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            2059..3039
                     /locus_tag="RO454_004748"
     CDS             2059..3039
                     /locus_tag="RO454_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDY0936459.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(3622..>4717)
                     /locus_tag="RO454_004749"
     CDS             complement(3622..>4717)
                     /locus_tag="RO454_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDY0936460.1"
                     /translation="GYCNSLCYHGKLQPKRGMEKGTIFPAMGYLHIDGRGMKPNGGSR
                     YNPLEAETIAAWLVAHKDDIERHYGEPLYKVVGVVTPFSAQVNAIKTSLRKLEINGKD
                     EQGSLTVGTVHSLQGAERAIVLFSPVYSKHEDGRFLDSNSSILNVAVSRAKDSFLVFG
                     DMDLIEMQPAFSPRGLLAKYLFSSDNNALQFEFQKRQDLISAHTQISTLHGVEQHDGF
                     LNKTLAGAQKKITIISPWLSWQKVEQTGFLASMALARSRGIDITVVTDKNCNIAHVDD
                     DKRQEKQHLLNDAVEKLNKMGIATKLVNRVHSKIVIEDEELLCVGSFNWFSAAREDKY
                     QRYDTSLVYRGEGVKNEIKAIYGSLDQRQL"
BASE COUNT         1368 a         1061 c         1036 g         1252 t
ORIGIN      
        1 ttttcacagg agacattata caaaaaatat aaaatacagc tacccgttgc caaagacact
       61 ataagcctgg caaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat
      121 tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg
      181 tattattact gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg
      241 gtggatatcg tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc
      301 aaaatggatg gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa
      361 aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg
      421 atcaatggac ggtacgcccg ggaatgttaa cgcattttag cagcaacggc acacgttacg
      481 gaccctacgt aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc
      541 gttacgactg gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg
      601 ttcatcgttg ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc
      661 aaacgacgct atacagcaaa cagaacgatt atcgctatgc aaaccataag aaatgggcga
      721 cggaaaatgc atttgttcta caataccata tgacgcccga tattacgcca tacatagaat
      781 atgactacct tgaccgtcag ggtgtttaca acggcagaga taatttatcg gaaaacagtt
      841 atcgcattgg tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaaca
      901 ataacggcgc ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa
      961 actcctgtgc catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt
     1021 ttaggtagcg caggtacggc atggtacaag ctggatacac aggccaaaga taaaaaatgg
     1081 acagcgttag ctgcattccc tggtggacca agagatcaag caacctcggc atttattgat
     1141 ggtgatctgt atgtgtttgg cggcattggc aaaaatagca agggcttgac tcaggtattt
     1201 aatgacgtac acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg
     1261 ccaatgggca tggcgggcca tgtgactttt gtacacaacg gcaaagctta tgttactggc
     1321 ggtgttaacc agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat
     1381 tcaaccgcta tagataaaat caacgctcac tattttgaca aaaaagcaga agattatttc
     1441 ttcaataagt ttctgttgtc ttttgatccc tcaacccagc aatggagtta cgctggcgaa
     1501 tctccctggt acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt
     1561 attaatggcg aagccaaacc cggattgcga acggatgccg tatttgaact tgatttcacc
     1621 ggtgataatt taaaatggaa taggcttgct cccgtctcat caccagatgg cgtcgctggc
     1681 ggttttgcgg ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt
     1741 tcacgagaaa attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat
     1801 agcactgata ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa
     1861 ggtcgggcct acggagtatc attgccctgg aataatagtc tattgattat tggcggtgaa
     1921 tctgcaggcg gcaaagcggt gacggattca gttttgatct ctgtgaagga taataaagtc
     1981 acagtacaaa actaacgctt cagggccccg gtaaagggcc ctgctatcaa tttgtcattt
     2041 caattacgcg aaaattatat gaacgcaata atatcgccca attattacta tgttcttacc
     2101 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2161 gatgcgcctc atcccagaat caaacaatta gcgagattta cgcatacgca tcccggaggc
     2221 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2281 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2341 caggcactgc atattgcacg gaaattactg cccttcattc ctgataatgc agggattctc
     2401 atcgtcccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaggg gacatattca
     2461 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatacccc gctctaccag
     2521 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcataacaa attcctcggc
     2581 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     2641 cactttaatc atatggttga agcctttcgt agggatctaa aacaattcca ttctcagctt
     2701 aataatatta ctgccgcgcc gtggttttgc ggcgatacca cctggtactg gaaagaaaat
     2761 ttccctcatg cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     2821 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagat
     2881 ccggacgatt taagcacggg atatttcggt tcagcgtacc ggtcagcgga gaactggacg
     2941 acgtcactgc gaagcagtca tttcagcgcg gcagcccgtc gggggattat ttctgacaga
     3001 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3061 gatgtccttg tccattccca gagcagacaa caaaaaacaa atgagatcaa ggtaatatct
     3121 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3181 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3241 catccgcgcc ttttcggtta tggctcggaa atctacgaac atctacacag tttactggaa
     3301 cgttttgggc cacagcgttc gcagcaccgt cctgatatgc cgttctggcg attataagtc
     3361 gacggattct ggcaaataga cgatgatgat atgcttaatg atgttcaaag aaattaggcc
     3421 attattatat tattgcttgc attttctttc attcctaaaa gcacccgcca ttaatcccag
     3481 gcgcattatg caaaggaaaa tgattaggta aacaataaga tttataatca ggaaaggcga
     3541 ctcgcaataa aacggaaagt cgccttaaca ttggtaaaat atgcccttta ctactcctgt
     3601 atcatgaacg gctcatcaca tttaaagctg acgttgatcc agactaccat atattgcctt
     3661 tatttcattt tttacgcctt cgccacggta caccaaagac gtatcgtatc gttgatactt
     3721 atcttcccgt gcagcactga accagttaaa agatccgaca cacagcagtt cttcatcctc
     3781 aatcacaatt ttgctgtgga cacgattaac caatttcgta gcgataccca tcttattgag
     3841 cttttcaaca gcatcattca gcagatgttg tttctcctga cgcttatcat catcgacatg
     3901 cgctatattg cagtttttat ccgtcaccac ggtaatatca atgccccggg aacgggccaa
     3961 agccattgat gccagaaatc ccgtttgctc cactttttgc caagataacc aaggggaaat
     4021 aatcgttatt ttcttttgag ctccggcaag agtcttgttc aaaaatccat catgctgctc
     4081 cacaccatgc agcgttgaaa tttgtgtatg tgcggaaatt aaatcctgtc gtttctgaaa
     4141 ctcaaattgc aacgcattat tatctgaaga aaagagatat tttgccaata accctcgcgg
     4201 cgaaaatgcg ggctgcatct cgataaggtc catatcgcca aataccagaa aactatcttt
     4261 agcgcgtgag acagcaacat tgaggatact gctgttgcta tcaagaaacc tgccatcttc
     4321 atgttttgag tagacaggag aaaagagaac aatcgccctt tccgccccct gcaaggagtg
     4381 cacagtcccc actgtcagtg aaccctgctc gtctttgcca ttaatttcca gcttacgcaa
     4441 tgacgttttg atagcattaa cctgcgccga aaatggcgtc acaacaccaa caactttgta
     4501 cagtggttcc ccgtaatggc gctctatatc atccttatgt gccaccagcc atgctgctat
     4561 tgtttcagct tcaagagggt tataacggct tcctccattt ggcttcatgc ctctaccatc
     4621 aatatgtaaa tatcccattg cggggaaaat cgtccctttt tccatccctc ttttaggctg
     4681 caacttaccg tgatagcaaa gtgagttaca gtagccg
//
LOCUS       DAPQZD010000256         4716 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPQZD010000256 DAPQZD010000000
VERSION     DAPQZD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.259"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="RO454_004750"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="RO454_004750"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDY0936461.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(858..1694)
                     /gene="kduI"
                     /locus_tag="RO454_004751"
     CDS             complement(858..1694)
                     /gene="kduI"
                     /locus_tag="RO454_004751"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDY0936462.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1981..3162)
                     /locus_tag="RO454_004752"
     CDS             complement(1981..3162)
                     /locus_tag="RO454_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDY0936463.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3417..4646
                     /locus_tag="RO454_004753"
     CDS             3417..4646
                     /locus_tag="RO454_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDY0936464.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1228 a         1203 c         1042 g         1243 t
ORIGIN      
        1 ttatgatgat ctacaggtat aaaaaaccct gccatgcgac agggtcataa aagtaagagg
       61 aatgaattaa cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc
      121 tgaagcgctg gaggcaagga acaccaccgg ccccatcagg tcactcggca gtccccaacg
      181 gccagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg
      241 agtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcgttc gccatcaatc gcgtcacgcc catcacaccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
      421 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcttt ttcgctgaat tcgagagcgt cttcgcggcg
      541 aatcaatccg gcgttattca ccaggatatc aatatgacca aactccgcta ccgcgcgatc
      601 cagcagtgct ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct tgatggtttc agtcggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaaa
      901 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
      961 tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt
     1021 tcttgcggtt gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
     1081 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga
     1141 gccagctccg tcagccccat actcaattgg caggtttcca gcacatccgg gacaaaatat
     1201 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
     1261 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
     1321 ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca
     1381 tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca
     1441 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
     1501 ttaccaactt ccccgccaac ggaaaccgtt ttagttaccg gcataatgcc gccaacaata
     1561 atacggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
     1621 aaaaattcat tgcgcagccc ctgggtatcc agcgtttttg cgtgcgcgct gtggatgctc
     1681 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacaataat
     1741 aaaacactgt ttcgttttct ctatgagggg atgatatgcc catgggatag ggttttcaat
     1801 taaaaataaa acaatgtttc attttcaagt taaggattaa aaaaagtgca gtgtcgatca
     1861 cgaatatgtc atgagaatgg aaaaatcagg gagccctgaa aaggtatgtc gactcaccag
     1921 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg taatgcagag
     1981 ctactcgtca cgttcaatgg tcaatgccac accctggcct ccgccgatac aaagcgttgc
     2041 cagtcctttg cgggcattac gtttcaccat ttcatgaaca agagaaacca ggattcggca
     2101 accggaagcg cctatcgggt gaccgagtgc gatcgcacca ccattaacat tgacccgacg
     2161 ctcatcccat tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
     2221 ctcgataaga tcgacatcag ccaactgcca gccaacacgc tccaggcaac ggcgggtcgc
     2281 atacaccggc gcaattccca tcaatgccgg atccacacca acgctggcaa atgcgcggat
     2341 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattactgc
     2401 agctgcccca tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa
     2461 tgaaggattt aaacgggcta agccttctgc gctggtgtca gtgcgtggct gttcatcggt
     2521 atcaacaacc aacgtctgac cgttactttg ggttattacc gggacgatct catctttaaa
     2581 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
     2641 caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
     2701 atcattgaag gcatcccaca acccatcatg cacaagacta tcaaccaact ggctattgcc
     2761 aagctgtgcg ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
     2821 gccaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
     2881 tgctttaagc ccggaaccac aaacgtcatt aatagtgatt gcagaaacgc tattaggaag
     2941 accaccttta atagccgatt gtcttgccgg attctgccct gcccctgcag tcaacacctg
     3001 accaagaatc acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt
     3061 gacgaccagg ctgccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc
     3121 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
     3181 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgac
     3241 cattatcaca aaggaaatac gcctgagtag tagtcagaga cataactggc acgtaaggtt
     3301 tgcaaccact aacccaccaa tagagaggta gataaggcgt taatctccca tacttaacct
     3361 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
     3421 ctaatatttg gtcaaaagaa gaaactctgt ggagtttcgc actctacggc acagccgttg
     3481 gtgcaggcac gctcttcctt cctattcagt taggttcggc gggggctgtg gtcctgttta
     3541 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
     3601 tcctctcatc gaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg
     3661 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
     3721 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
     3781 ttgatcttcg catccgtatg ctagtgagtc tgggggttgt attaatcctg aatctcattt
     3841 ttctgatggg acgccatgcc actattcggg taatgggatt tttggtattc ccattgattg
     3901 cctatttctt atttctttct atttacctgg tcggtagttg gcaacctgat ctattaacaa
     3961 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
     4021 ttttcgcttt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
     4081 atggcgaaca tgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
     4141 gcataagtgt attgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg
     4201 aagcggctaa agaagaaggg gttaccattt tatcggcgct ttctatgctg ccgaacgccc
     4261 cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
     4321 tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca acattacagc
     4381 aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc
     4441 tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
     4501 gcggcccgct cattgccatg atacttttca tcatgcctac gctgtcaacg tatctcatcc
     4561 cggcgcttaa accctggcgt tccatcggaa atctgattac cctgatcgtg ggtatcctgt
     4621 gcgtatcggt aatgttcttt agctaatatc cgttgtggat ggttcaaaat tgcttcaatt
     4681 ttgaaccatc ccagttttca taatgttcat ttgtta
//
LOCUS       DAPQZD010000257         4669 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPQZD010000257 DAPQZD010000000
VERSION     DAPQZD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.260"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            151..1410
                     /gene="codB"
                     /locus_tag="RO454_004754"
     CDS             151..1410
                     /gene="codB"
                     /locus_tag="RO454_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDY0936465.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1400..2683
                     /gene="codA"
                     /locus_tag="RO454_004755"
     CDS             1400..2683
                     /gene="codA"
                     /locus_tag="RO454_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDY0936466.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(2804..3415)
                     /gene="lacA"
                     /locus_tag="RO454_004756"
     CDS             complement(2804..3415)
                     /gene="lacA"
                     /locus_tag="RO454_004756"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDY0936467.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGCHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(3481..>4669)
                     /gene="lacY"
                     /locus_tag="RO454_004757"
     CDS             complement(3481..>4669)
                     /gene="lacY"
                     /locus_tag="RO454_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDY0936468.1"
                     /translation="MGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLL
                     SDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPA
                     VEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALILAV
                     LLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD
                     QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLVAGTI
                     MSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCF
                     FKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVN
                     EVA"
BASE COUNT         1173 a         1136 c         1196 g         1164 t
ORIGIN      
        1 cactgttttt acacagataa gtttcccccg cctttttgta ctcattcata taagaaatat
       61 atccccatac gaaaacgatt gctttttatc ttcagatgaa tagaatgcgg cggatttttt
      121 gggtttcaaa cagcaaaaag ggggaatttc gtgtcgcaag ataacaactt tagccagggg
      181 ccagtcccgc agtcggcgcg gaaaggggta ttggcattga cgttcgtcat gctgggatta
      241 accttctttt ccgccagtat gtggaccggc ggcactctcg gaaccggtct tagctatcat
      301 gatttcttcc tcgcagttct catcggtaat cttctcctcg gtatttacac ttcatttctt
      361 ggttacattg gcgcaaaaac cggcctgacc acccatcttc ttgctcgctt ctcgtttggt
      421 gttaaaggct catggttgcc ttcactgctg ctgggcggca cccaggttgg ctggtttggc
      481 gttggcgtgg cgatgtttgc tattccggtg ggcaaggcaa ccgggctgga tattaatttg
      541 ctgattgccg tctccggttt actgatgacc gtcaccgtct tctttggcat ttcggcgctg
      601 acagttcttt cggtgattgc ggttccggct atcgcctgtc ttggcggtta ttccgtgtgg
      661 ctggccgtta acggcatggg cggcctggac gcattaaaag cggtcgttcc cgcacaaccg
      721 ttagatttca atgtcgcgct ggcgctggtt gtggggtcat ttatcagtgc gggcacgctc
      781 accgctgact ttgtccggtt tggtcgtaat gccaaactgg cggtgctggt ggcgatggtg
      841 gcctttttcc tcggcaactc gttgatgttt attttcggtg cagcgggcgc ggcggcactg
      901 gggatggcgg atatctctga tgtgatgatt gctcagggcc tgctgctgcc tgcgattgtg
      961 gtgctggggc tgaatatctg gaccaccaac gataacgcgc tctatgcgtc gggtttaggt
     1021 ttcgccaaca ttaccggtat gtcgagcaaa accctttcgg tgattaacgg tattatcggt
     1081 acggtctgcg cattatggct gtataacaat tttgtcggct ggttgacctt cctttcggca
     1141 gctattcctc cggtgggcgg cgtgatcatc gctgattatc tgatgaaccg tcgccgctat
     1201 gagcactttg cgaccacgcg tatgatgagt gtcaattggg tggcgattct ggctgtcgcg
     1261 ctggggattg ccgcaggcca ctggttaccg ggaattgttc cggtcaacgc ggtattaggt
     1321 ggcgcgctga gctatctgat ccttaacccg attttgaatc gtaaaacgac agcagcaatg
     1381 acgcatgtgg aggctaacag tgtcgaataa tgctttacaa acaattatta acgcccggtt
     1441 acccggcaaa gaggggctgt ggcagattca ttttcacgac ggaaaaatca gcgccattga
     1501 tgcgcaatcc ggcgtgatgc ccgtaactga aaacagcctg gatgccgaac aaggtttagt
     1561 tttaccgccg tttgtggagc cgcatattca cctggacacc acgcaaaccg ccggacaacc
     1621 gaactggaat cagtccggca cgctgtttga aggcattgaa cgctgggccg agcgcaaagc
     1681 gttattaact catgacgatg tgaaacaacg cgcatggcaa acgctgaaat ggcagattgc
     1741 caacggcatt cagcatgtgc gtacccatgt cgatgtttcg gacgcaacgc tgactgcgct
     1801 gaaagcaatg ctggaagtga aacaggaagt tgcgccgtgg attgatctac aaatcgtcgc
     1861 cttcccgcag gaagggattt tgtcgtatcc caatggtgaa gcgttgctgg aagaggcgtt
     1921 acgcttaggg gcagacgttg ttggggcgat tccgcatttt gaatttaccc gtgaatatgg
     1981 cgtggagtcg ctgcataaaa ccttcgccct ggcgcaaaaa tacgaccgtc tcatcgatgt
     2041 tcactgtgac gagattgatg atgagcaatc acgctttgtc gaaaccgttg ctgccctggc
     2101 gcaccgtgaa ggcatgggcg cgcgagtcac cgccagccat actacggcaa tgcactccta
     2161 taacggggcg tatacttcgc gcctgtttcg tttgctgaaa atgtccggta ttaactttgt
     2221 cgccaacccg ttggtcaata ttcatctgca aggacgtttc gatacgtatc caaaacgtcg
     2281 cggcatcacg cgcgttaaag agatgctgga gtccggcatt aacgtctgct ttggtcacga
     2341 tgatgtcttc gatccgtggt atccgctggg aacggcgaat atgctgcaag tgctgcatat
     2401 ggggctgcat gtttgccagc tgatgggcta tgggcagatt aacgacggcc tgaatttaat
     2461 cacccaccac agtgccagaa cgttgaattt gcaggattac ggcattgccg ccggaaacag
     2521 cgccaacctg attatcctgc cggctgaaaa tgggtttgac gcgctgcgcc gtcaggttcc
     2581 ggtgcgctat tcggtacgtg gcggcaaggt gattgccagc acaaaaccgg cacaaaccac
     2641 cgtatatctg gagcagccag aagccatcga ttacaaacgg taatcggctg ggttaccgcg
     2701 agcgtagttt atgccggatg cggcgtgaac accttatccg gcctacgtag atcactgaaa
     2761 ttgtaggcct gataagcgga gcgcatcagg caattttgct tatttaaact gaagactcaa
     2821 ctttaaaatc tttgaaataa tattgcttat cccggtcgtt tatttcgcga ataacccgac
     2881 aaggaacgcc tgcagccacg acgtttggtg gaatgtcttt tgtgacgaca ctacccgcgc
     2941 caataacaga attatccccg atggtgatgc ctggattaat aaccacatga catccgatcc
     3001 agacgttatt gccaatcgtt atcggaaaag agtacatctc tccgtttttt ctcaattcat
     3061 ggtgtaccgg gtgtcccgta acggaaagag taacgttggg tgcaatcagt acgttatcac
     3121 cgatcgttac cgtgtagtcg tcgacaatgg ttaaattgaa atttgcataa aaattgcggc
     3181 ctatatggat gttggaaccg taagagaaat agacgggggg gtctacccag gcgttttccc
     3241 ctaccgtggc aaacatttct ttaatcagac tttccctttt ttcaaattct gatggatgcg
     3301 agtgattaaa ctcatacatt aacgtttttc cacgaagtct tttttcaggt aagccttcgc
     3361 acatatcggt aaatagcttg cctgctttta ttctttctgt catcgacatg ttcattgcga
     3421 tcattccgcc ctgatatgtt ggtcggataa ggcgctcgcg ccgcatccga cattgattgc
     3481 ttaagcgact tcattcacct gacgacgcag tagagaaagc gggccggggc cgctaagcgt
     3541 gaacacggaa attaaggtga agcccagcgc caccagaccc agcaccagat aagcgccctg
     3601 gaaaccgatg ctttcataca tattgcccgc cagtacagac ataaaaatca tcgccagttg
     3661 cttaaagaag cagaaacaga ccagataaat cgtcgctgaa aaacgcactt caaactggct
     3721 ggtaatatat ttaaagcagc ccaccagcag gaacggtact tcaaacatat gcagcgtttt
     3781 cagaataacc acttccagcg ctgaggtggc gaacgatgag ccaataatac gtacagacat
     3841 aatagtgcca gccaccagca gggcattttt cccaccgatg cgattaatga tcagtggcgc
     3901 aaagaacata atcgaggcat taagtaattc gcccattgtc gttacgtagc caaatacccg
     3961 cgtaccctgt tcaccggtag caaaaaacga agtaaagaaa ttagcaaact gttggtcaaa
     4021 aacatcgtag gtgcaggaaa cgccaataac atacagtgac aaaaaccaca gttttggctg
     4081 tctgaacagt tccagcgcca gcttaaggct aaatgccgaa tggttggcac ctaccgcatt
     4141 ggcaaccgtg gctgaagagg gcgcatccgt tttggcgaaa aagagtaaaa cggcgaggat
     4201 gagtgcacag ccagaaccca gccagaaaac aaactgatta ttgatggtga acatgatgcc
     4261 gacaatcgag gcacacagcg cccagccaac acagccaaac atccgcgcgc gaccaaattc
     4321 gaaatgactg cgacggctga ctttctcgat aaatgcctct actgctggcg caccggcgtt
     4381 aaaacaaaag ccaagataaa taccaccaac aatcgatcct actaaaatgt tgtattgtaa
     4441 cagtggtccg aagataaaaa taaagaacgg cgcaaacatc actaacatgc cggtaataat
     4501 ccacagcagg tatttgcgca gcccgagttt gtcagaaagc agaccaaaca gcggttggaa
     4561 taatagcgag aacagagaaa tagcggcaaa aataataccc gtatcacttt tgctgatatg
     4621 gttgatgtca tgtagccaaa tcgggaaaaa cgggaagtag gctcccatg
//
LOCUS       DAPQZD010000258         4649 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPQZD010000258 DAPQZD010000000
VERSION     DAPQZD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.261"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..2888
                     /gene="sat"
                     /locus_tag="RO454_004758"
     CDS             <1..2888
                     /gene="sat"
                     /locus_tag="RO454_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HDY0936469.1"
                     /translation="TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNING
                     QGFTFKGAGIDIGKESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILN
                     EEGTFNNIYLASGNGKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATD
                     DGTTITNSDTTKEAVLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVN
                     TKNDVEVSNASLTMQGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNS
                     DHKSNNQQTSFDQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKN
                     AYIDNLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGAT
                     FLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFR
                     LSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTA
                     YRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDD
                     SAFVLRANLAQADQLVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVF
                     KATTRSIGFSDVTPVIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAE
                     VNNLNKRMGDLRDINGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLF
                     TGVTMTYTDSHAGSDAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFA
                     GLGTRDYSSHSWYAGAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMK
                     DKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGE
                     KDGRMLMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            3160..3420
                     /locus_tag="RO454_004759"
                     /pseudo
     CDS             3160..3420
                     /locus_tag="RO454_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3485..4636
                     /locus_tag="RO454_004760"
     CDS             3485..4636
                     /locus_tag="RO454_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDY0936470.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT         1478 a          977 c         1056 g         1138 t
ORIGIN      
        1 gtactcaaga aaaaataact aaagacaaag atattgtgtt cacaaatggg ggaaatgtcc
       61 tgtttaagga taatttggat tttggtagcg gtggtattat ctttgacgaa ggccatgaat
      121 ataacataaa cggtcaggga tttacattta aaggagcagg aattgatatc ggaaaagaaa
      181 gcattgtaaa ctggaatgca ttgtattcca gtgatgatgt tttacacaaa ataggccccg
      241 gtactctgaa tgttcaaaaa aaacaggggg caaatataaa gataggtgaa ggaaatgtta
      301 ttcttaatga agaaggaaca tttaacaata tataccttgc aagcggaaat ggtaaggtaa
      361 tactaaataa agataattcc cttggcaatg atcaatatgc ggggatattt tttactaaac
      421 gtggtggcac gctagattta aatggacaca atcagacttt tactagaatt gccgccactg
      481 acgatggaac aacaataact aactcagata caacgaaaga agccgttctg gcaatcaata
      541 acgaagactc ctacatatat catgggaaca taaatggcaa tataaaacta acgcacaata
      601 ttaattctca ggataagaaa actaatgcaa aattaattct ggatggtagt gtcaacacaa
      661 aaaatgatgt tgaagtcagt aatgccagtc ttaccatgca aggccatgca acagagcatg
      721 caatattcag aagcacagcg aatcattgct ccctggtatt tctttgtgga acggactggg
      781 tcaccgtttt gaaagaaaca gagagttcat ataataaaaa gttcaattct gatcacaaaa
      841 gtaataatca gcagacctca tttgatcagc cagactggaa aaccggggtg tttaaatttg
      901 atacattaca cctgaacaat gctgactttt caatatcacg caatgccaat gttgaaggaa
      961 atatatcagc aaataaatca gctatcacaa tcggcgataa aaatgcttac attgataatc
     1021 ttgcagggaa aaatattact aataatggtt ttgacttcaa acaaactatc agtactaatc
     1081 tatccatagg agaaactaaa tttacaggtg gcatcactgc acataacagc caaatagcca
     1141 taggtgatca agctgtagtt acacttaatg gtgcaacctt tctgaataat actcctataa
     1201 gtatagataa aggagcaaaa gttatagcac aaaattccat gttcacaaca aaaggtattg
     1261 atatctccgg tgagctgact atgatgggaa tccctgaaca gaatagtaaa actgtaacgc
     1321 cgggtctcca ctacgctgct gatggattca ggctgagtgg tggaaatgca aatttcattg
     1381 ccagaaatat ggcatctgtc accggaaata tttatgctga tgatgcagca accattactc
     1441 tgggacagcc tgaaactgaa acaccgacta tatcgtctgc ttatcaggca tgggcagaga
     1501 ctcttttgta tggctttgat accgcctatc gaggcgcaat aacagccccc aaagctacag
     1561 ttagcatgaa taatgcgatc tggcatctaa atagccagtc atcaattaat cgtctagaaa
     1621 caaaagacag tatggtgcgt tttactggtg ataatgggaa gtttacaacc cttacagtgg
     1681 acaaccttac tatagatgac agtgcatttg tgctgcgtgc aaatctggcc caagcagatc
     1741 agcttgttgt caataaatcg ttgtctggta aaaacaacct tctgttagtc gacttcattg
     1801 agaaaaatgg aaacagcaac ggactgaata tcgatctggt cagcgcacca aaaggaactg
     1861 cagtagatgt ctttaaagct acgactcgga gtattggctt cagtgatgta acaccggtta
     1921 tcgagcaaaa gaacgataca gacaaagcaa catggactct gatcggctat aaatctgtgg
     1981 ccaacgccga tgcggctaaa aaggcaacat tactgatgtc aggcggctat aaagccttcc
     2041 ttgctgaggt caacaacctt aacaaacgta tgggtgatct gcgtgacatt aacggtgagt
     2101 ccggtgcatg ggcccgaatc atgagcggaa ccgggtctgc cggcggtgga ttcagtgaca
     2161 actacaccca cgttcaggtc ggtgcggata acaaacatga actcgatggc cttgacctct
     2221 tcaccggggt gaccatgacc tataccgaca gccatgcagg cagtgatgcc ttcagtggtg
     2281 aaacgaagtc tgtgggtgcc ggtctctatg cctctgccat gtttgagtcc ggagcatata
     2341 tcgacctcat cggtaagtac gttcaccatg acaacgagta taccgcaact ttcgccggcc
     2401 ttggcaccag agactacagc tcccactcct ggtatgccgg tgcggaagtc ggttaccgtt
     2461 accatgtaac tgactctgca tggattgagc cgcaggcgga acttgtttac ggtgctgtat
     2521 ccgggaaaca gttctcctgg aaggaccagg gaatgaacct caccatgaag gataaggact
     2581 ttaatccgct gattgggcgt accggtgttg atgtgggtaa atccttctcg ggtaaggact
     2641 ggaaagtcac agcccgcgcc ggccttggct accagtttga cctgtttgcc aacggtgaaa
     2701 ccgtactgcg tgatgcgtcc ggtgagaaac gtatcaaagg tgaaaaagac ggtcgtatgc
     2761 tcatgaatgt tggtctcaac gctgaaattc gcgataatct tcgcttcggt cttgagtttg
     2821 agaaatcggc atttggtaaa tacaacgtgg ataacgcgat caacgccaac ttccgttact
     2881 ctttctgata acagcccggg ccgcgtttgc ggcccttctt ctaccggaga gaatatgtat
     2941 taccctgtga cagactatat cgctcttgct ctcattatta gctttctttt tctgacatta
     3001 tttatctgcc tgttatgcct taaacatgag cgaataaaaa aagaaactat caggcaaaaa
     3061 aatgcacata ttctggatca tggctggaat gcaactgagg tagcctgagt ttaacggaca
     3121 ctccttcctg aaatagaatg gcatcagaag gagctaataa tgagcagaaa aacccaacgt
     3181 tactctaaag agttcaaagc cgaagctgtc agaacggttc ttgaaaatca actttcgatc
     3241 agtgaaggcg cttcccgatt atctcttcct gaaggcactt taggacaatg ggttaccgcc
     3301 gccagaaaag ggctcggtac tcctggttcc cgcacggtgg ctgaactgga atctgaaatt
     3361 ctgcaactgc gtaaggcgtt aaatgaagct cgccttgagc gagatatatt aaaaaaagca
     3421 actgtagatt caattggtca acgcaacagt tatgtgaaaa catggggttg cggaggtttt
     3481 ttgaatgaga cgaacattta cagcagagga aaaagcctct gtttttgaac tatggaagaa
     3541 cggaacaggc ttcagtgaaa tagcgaatat cctgggttca aaacccggaa cgatcttcac
     3601 tatgttaagg gatactggcg gcataaaacc ccatgagcgt aagcgggctg tagctcacct
     3661 gacactgtct gagcgcgagg agatacgagc tggtttgtca gccaaaatga gcattcgtgc
     3721 gatagctact gcgctgaatc gcagtccttc gacgatctca cgtgaagttc agcgtaatcg
     3781 gggcagacgc tattacaaag ctgttgatgc taataaccga gccaacagaa tggcgaaaag
     3841 gccaaaaccg tgcttactgg atcaaaattt accattgcga aagcttgttc tggaaaagct
     3901 ggagatgaaa tggtctccag agcaaatatc aggatggtta aggcgaacaa aaccacgtca
     3961 aaaaacgctg cgaatatcac ctgagacaat ttataaaacg ctgtactttc gtagccgtga
     4021 agcgctacac cacctgaata tacagcatct gcgacggtcg catagccttc gccatggcag
     4081 gcgtcatacc cgcaaaggcg aaagaggtac gattaacata gtgaacggaa caccaattca
     4141 cgaacgttcc cgaaatatcg ataacagacg ctctctggga cattgggagg gcgatttagt
     4201 ctcaggtaca aaaaactctc atatagccac acttgtagac cgaaaatcac gttatacgat
     4261 catccttaga ctcaggggca aagattctgt ctcagtaaat caggctctta ccgacaaatt
     4321 cctgagttta ccgtcagaac tcagaaaatc actgacatgg gacagaggaa tggaactggc
     4381 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
     4441 tccttggcag cggggaacaa atgagaacac aaatgggcta attcggcagt actttcctaa
     4501 aaagacatgt cttgcccaat atactcaaca tgaactagat ctggttgctg ctcagctaaa
     4561 caacagaccg agaaagacac tgaagttcaa aacaccgaaa gagataattg aaaggggtgt
     4621 tgcattgaca gattgaatct acaaccgcg
//
LOCUS       DAPQZD010000259         4646 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPQZD010000259 DAPQZD010000000
VERSION     DAPQZD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.262"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..746)
                     /locus_tag="RO454_004761"
     CDS             complement(<1..746)
                     /locus_tag="RO454_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDY0936471.1"
                     /translation="MNNQTIRTSVPALDKTVRVCNYLFSSPGATFSQIQQDLDLPKSS
                     TSSLLNALVEHHLLRQERGRFFLGLKLYEWGNRSLEQFDISKVARPVLEKLRDSTDLI
                     CHLGILDEVAPIYILKLESTQPIGIRTWVGKKLPLHSSGVGKALCAWLPQDVIDTLLP
                     EETLPRFTQTTITRKSDLIQEFARIRERGWAFDNAEDSPGIYCIAAPVFNQTREVIAA
                     ISISGVEIQIPKEKIEYYSTLVLDACQELS"
     gene            986..1885
                     /locus_tag="RO454_004762"
     CDS             986..1885
                     /locus_tag="RO454_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDY0936472.1"
                     /translation="MLTAFRGIFPPVPTIVDAQGKLDKAGMARMIDHVLENGANGMLI
                     LGSGGEFSQFSSQQRKEIAAFCLSHVDGKVPVLVGIACAGTAETIDLGHHAQEHGADG
                     VMVVNPYYAKLSDDARFMHYKSIADSLKIPVFLYNFPDLTGQDIGLEVITRLAREVPN
                     IVGIKDTIDNISHTREVINRVHAFRPDFIIFSGYDEYMLDTLLLGGHGGIPATFNFAP
                     NITRGIYQAVMENDLDKARYFQQQLAKLSPLYTLEQPFFGVIKTAIKLSGLDISTAVM
                     APALPLSDDKVARVKAILEHIHQ"
     gene            1902..3419
                     /locus_tag="RO454_004763"
     CDS             1902..3419
                     /locus_tag="RO454_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDY0936473.1"
                     /translation="MNKTPILKLNNSDDVVIARTELPAGSWLESESMETRDLIPAGHK
                     IALRDIPAGSAVKRYGQIIGFATRDIRQGEHIHVHNLGMGSFERDYAWGIDSHLPEKD
                     VHQDTFMGIRRKDGRVATRNYIGVLSSVNCSATVVRAIEDHFRTRGLSDYPNVDGIVA
                     LPQSFGCAFGSDSEMMKVMRRTMAGYATHANFAGIVIVGLGCESFQIKDMMNAHGLHE
                     SPVFHTMTIQESGGTRNAIEKGISFILDMLPEANNVKREPVPVSEIILALECGGSDSY
                     SGISANPALGYAVDRLVRQGGTAILSETSEIYGAEHLLTRRAISREVGEKLIRRIKWW
                     EEYCKRTGSEMNNNPSAGNKAGGLTTVLEKSLGGIAKAGTTSLMDVYEYAEPVTAKGL
                     VFMDTPGYDPMAVTGQIAGGANLLCFTTGRGSAFGAKPTPCLKLSTNNALWQRQRDDM
                     DLNCGTIVDGEETIEQVGERIYQRIIELASGHRSRSEELGYGSQEFVPWIMNSIM"
     gene            3497..4510
                     /locus_tag="RO454_004764"
     CDS             3497..4510
                     /locus_tag="RO454_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDY0936474.1"
                     /translation="MERKPVLGIFLGEAAGIGPELVAKVVADGTVYKYCRPVLIGDAR
                     VLALGQKIAGVHFQWNVISDPTEADWTTGNVPLLDLKNFNPEKLTMGTIDTDSGYATG
                     ESLVTCMSLLKSGKIDGFVFAPLNKEAFKKGGWDIEDEHYLFAEQLGYLDKPRGLLNV
                     LGDLWVFRVTGHIPFKDIASHITPENVSRSIQLCYDTLRMAAVENPRIAVAALNPHAG
                     DGGTCGKEEIDVLIPVIEGFREKGMDMYGPVPGDTVFIHAFNHEYDAVVTLFHDQGQI
                     ATKLHSFDVGITVAAGLPFAITTPEHGTAFDIAGKGIAKTLATERAIAVAARMASTVH
                     KDN"
BASE COUNT         1290 a          976 c         1112 g         1268 t
ORIGIN      
        1 tccgatagtt cttgacacgc atcaagaacc aaagttgaat aatattcaat tttttcttta
       61 ggtatctgaa tttccacgcc actaatgctg atagctgcaa ttacttcacg agtctgatta
      121 aataccggag cagcaataca ataaattccc ggtgaatcct ctgcgttatc aaaagcccat
      181 ccacgttcac gaatacgtgc aaattcctgt attagatcag atttacgagt gatagttgtt
      241 tgcgtaaatc gtggcaacgt ttcctcaggc aacaatgtgt caatgacgtc ttgaggtaac
      301 cacgcacata aagccttacc aactccagaa ctatggagtg gcaacttctt gcctacccac
      361 gttctgatgc caattggttg tgtactctcg agtttcaaaa tatagattgg agcgacttca
      421 tcgagaatgc ccagatggca gataagatca gtactgtctc tcagtttttc tagaaccggc
      481 ctcgctacct tactgatgtc aaactgctcc agagatcgat tcccccattc atacaatttc
      541 agcccaagaa aaaaacgccc tctttcctga cgtaacagat gatgttcgac aagggcattc
      601 agtaaagatg atgtgctgct ttttggcaga tccagatcct gctggatctg gctgaaggtg
      661 gcgcctggag agctaaaaag gtaattgcag actctgacag ttttatcgag tgctggtacc
      721 gatgttctga tggtttgatt attcataaaa aaatatgatt ccttacaata ctgaatagta
      781 taccccattc ccccttgatg cgcagcggcg cttagcacac atattataca tggcctccgc
      841 gtaccggcat gcctctataa acctgactta tttcacactt ttcatatcgc attgtaaaaa
      901 catttgacat gcaaaacctg aatttgattc actttattca gcataaagaa cttgtgttcc
      961 atatacagaa cttagtgaga gtgatatgtt gacagcgttt agaggtattt tcccgccagt
     1021 tcctacaatt gttgacgcac aagggaaact agataaggct ggcatggcca ggatgatcga
     1081 ccatgtttta gaaaatggtg caaacgggat gttgatcctt ggcagtgggg gagaattctc
     1141 acaattttct tcccagcaac gaaaagagat agctgcattt tgcttaagtc atgtagatgg
     1201 caaagtgcct gttctggtgg ggatcgcctg cgcgggaact gcagaaacta ttgatttagg
     1261 acatcatgca caggagcacg gagctgatgg tgtgatggtg gtgaatccgt attatgcaaa
     1321 actcagcgat gatgcccggt ttatgcatta caaatctatt gccgattcac ttaaaattcc
     1381 agtgtttctc tataactttc ctgatctgac cggtcaggat attgggcttg aggtcattac
     1441 ccggctggcc cgggaagttc caaatatcgt gggtatcaag gataccatcg ataatatcag
     1501 ccatacccgg gaagtcatca accgtgtaca tgcttttcgt ccggatttca ttatattcag
     1561 cggatatgac gaatatatgc tggataccct gttactgggc ggccatggtg gtattccggc
     1621 gacattcaat tttgctccaa atatcacacg tggtatttat caggccgtta tggaaaatga
     1681 tcttgataaa gcgcgttatt ttcagcaaca actggcaaaa ctgtctccgt tatacacact
     1741 ggagcagcct ttctttggcg tgataaaaac tgccatcaaa ctgagtggac tggacatatc
     1801 tactgcagtt atggcaccgg ctcttccgct cagtgatgac aaggtcgcca gggtgaaagc
     1861 cattctggaa cacattcacc agtgattacg aggatcgtaa gatgaataag actccgatac
     1921 tcaaattaaa taactcggat gatgtcgtta ttgccaggac agaacttcct gccggaagct
     1981 ggctggaatc tgaaagtatg gaaacacgtg accttattcc ggcagggcac aaaattgctc
     2041 ttcgggatat tccggccggc agtgccgtta aacgctatgg acagataatc ggttttgcga
     2101 cccgtgacat ccgacagggc gaacatattc atgtgcataa tctcggtatg gggagttttg
     2161 agcgtgatta tgcctggggg atagacagtc atcttccgga aaaggatgtt catcaggata
     2221 cctttatggg gatccgcaga aaagacggac gggtggcaac ccgtaattat attggtgtac
     2281 tgagttctgt aaactgtagt gcaaccgtag taagggcaat tgaagatcat ttccgtaccc
     2341 gtggtctcag tgattaccct aatgtggatg gtattgttgc cctcccacaa agttttggtt
     2401 gtgcctttgg ctctgacagc gaaatgatga aagtcatgcg gaggacaatg gcaggctatg
     2461 ccacgcacgc gaactttgcc gggatcgtga tcgtgggact gggatgtgaa tcattccaga
     2521 ttaaggacat gatgaatgcc cacggattac atgaaagtcc tgtattccat acaatgacta
     2581 ttcaggaaag cggcggaaca cgtaacgcta tagaaaaagg tatttccttc attctcgaca
     2641 tgctcccgga agccaacaat gtcaaacgag aaccagttcc ggtatcagaa atcattctgg
     2701 ctcttgagtg tggcggctct gatagctatt caggaatttc cgctaatccg gctctcgggt
     2761 acgcagttga tcgcctggtt cgccagggag gaacggctat tctgtcggaa acatcggaaa
     2821 tttatggagc agaacacctt ctgacacgcc gtgccattag tcgcgaagtt ggcgagaaac
     2881 taatccgcag aattaagtgg tgggaagagt actgtaaacg tacagggagt gaaatgaata
     2941 acaacccttc tgcaggtaat aaagcggggg gactgaccac tgttctggag aaatcgctgg
     3001 gagggatagc aaaagctggt acaaccagct taatggatgt gtatgaatat gctgaaccgg
     3061 tgacagcaaa agggcttgtc tttatggata cccccggata tgacccgatg gcagtaaccg
     3121 gacaaatcgc cggaggagcc aacctgctgt gttttaccac gggcagggga tctgcctttg
     3181 gcgcaaaacc cactccttgt ctgaaactgt caaccaataa cgcactatgg caacggcagc
     3241 gcgacgacat ggatttaaac tgtggcacga ttgttgatgg cgaagaaaca atagaacagg
     3301 ttggagaacg tatttatcag agaattattg agctggcttc aggtcatcgt agtcgaagtg
     3361 aagagctggg ttatgggagc caggaattcg ttccttggat aatgaactca atcatgtaat
     3421 aactacgggc ataatttgtt ttgcccggaa ataaggaaac aaactgaatc ctgtttttga
     3481 taattagagg tgaataatgg aacgtaaacc agtactgggc atttttttag gtgaagctgc
     3541 cggcattgga cctgaactgg ttgccaaagt cgtagctgat ggtactgtct ataaatattg
     3601 cagacctgtt ctgattggcg atgcccgggt acttgctctt ggacaaaaga ttgctggtgt
     3661 gcattttcag tggaatgtca tcagtgatcc cactgaagct gactggacta cgggaaatgt
     3721 accattactt gatttgaaaa attttaatcc cgaaaaacta actatgggca ccattgatac
     3781 cgactcaggt tatgcaacag gagaatcact ggtaacctgt atgtcattac ttaaatcagg
     3841 taaaattgac ggttttgtct tcgctccact caataaagaa gctttcaaga aggggggctg
     3901 ggatattgag gacgaacatt atctctttgc tgagcagtta ggatatttgg ataaacctcg
     3961 tggcctgttg aatgtccttg gtgacctctg ggtttttcgc gtgacgggac atattccatt
     4021 caaagatatt gccagccata ttacaccaga aaatgtcagt cgttcgattc agttatgcta
     4081 tgacacatta cgtatggcgg ccgttgaaaa tccacgaata gcggttgcag cacttaatcc
     4141 tcatgccggt gatggaggaa cctgtggtaa ggaagaaatt gatgttctca ttccggtcat
     4201 tgaaggcttc cgcgagaaag ggatggatat gtatggccca gtaccgggtg atactgtttt
     4261 tattcatgca tttaatcatg aatatgatgc tgtcgttacc ctgttccatg accagggaca
     4321 gattgccacc aagcttcatt cctttgatgt gggcataact gtagccgcag ggcttccatt
     4381 tgctattaca acaccggaac atggcactgc atttgatatt gcaggcaagg gaatagcaaa
     4441 aactctggcc acagagcggg ctattgcagt tgcagctaga atggcatcta cagtgcataa
     4501 agataattga gatctctaaa tcttagtgta tttatatatt cttttttgat tttattgaat
     4561 gaagttattt ttatgttatt ccgccttagc cataagccat ggttaaagcg gaataacaag
     4621 ccaaaaagcg tccctcgaag cacgtt
//
LOCUS       DAPQZD010000260         4561 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPQZD010000260 DAPQZD010000000
VERSION     DAPQZD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.263"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..599
                     /locus_tag="RO454_004765"
     CDS             <1..599
                     /locus_tag="RO454_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDY0936475.1"
                     /translation="IGYAVIRRTIFIVYPPILSNGMFNFIVMQTLFYLPFFILGALAF
                     IFPHLKALFTTPSRGCTLAAALAFVAYLLNLRYGSGDAWMYETESVITMVLGLWMVNV
                     VFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIF
                     VVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(607..2088)
                     /gene="clsC"
                     /locus_tag="RO454_004766"
     CDS             complement(607..2088)
                     /gene="clsC"
                     /locus_tag="RO454_004766"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDY0936476.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTCLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(2030..2563)
                     /gene="ymdB"
                     /locus_tag="RO454_004767"
     CDS             complement(2030..2563)
                     /gene="ymdB"
                     /locus_tag="RO454_004767"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDY0936477.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2658..2969)
                     /locus_tag="RO454_004768"
     CDS             complement(2658..2969)
                     /locus_tag="RO454_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0936478.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3090..3422)
                     /gene="csgC"
                     /locus_tag="RO454_004769"
     CDS             complement(3090..3422)
                     /gene="csgC"
                     /locus_tag="RO454_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDY0936479.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3482..3940)
                     /gene="csgA"
                     /locus_tag="RO454_004770"
     CDS             complement(3482..3940)
                     /gene="csgA"
                     /locus_tag="RO454_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDY0936480.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3981..4436)
                     /gene="csgB"
                     /locus_tag="RO454_004771"
     CDS             complement(3981..4436)
                     /gene="csgB"
                     /locus_tag="RO454_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDY0936481.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1163 a         1133 c         1009 g         1256 t
ORIGIN      
        1 gcatcggtta tgcggtaata agaagaacga tttttattgt gtatccaccc attctgagta
       61 acggcatgtt caattttatt gtcatgcaaa cgttatttta tttgccgttc tttatcctcg
      121 gcgcactggc tttcattttc cctcatctta aagccttgtt taccacgcct tctcgtggct
      181 gtacccttgc agcagcattg gcgtttgtcg cttatttact caacctgcgc tatggcagtg
      241 gcgatgcctg gatgtatgaa accgagtcgg tgatcaccat ggtcctcggt ctgtggatgg
      301 tgaatgtggt cttctccttt ggccaccgtt tgcttaactt ccagtcagcg cgggtgactt
      361 attttgttaa cgcatcgctg tttatctatc tggttcacca cccgttaacg ctgtttttcg
      421 gcgcatacat tacaccacac atcacctcca actggcttgg ttttctctgt ggcctgatat
      481 tcgtagtagg gattgcgata attctgtatg aaattcattt acgcatccca ttattgaagt
      541 ttttgttctc tggtaaaccg gttgttaagc gtgagaacga taaagcacct gcccgttaag
      601 ccgcgtttac aataaccatt ccacgggcaa tatcgacgcc agtttgacca taacccgctt
      661 ccagaaactg gtggcgggtt cttttttgag aataatctct ttctttgcat gacgatcaac
      721 ccagttgatc cgtccccacc tgtccagacg gagctgccag gccgcatcat actggctctg
      781 aataaagcgt ttatcaatta actgtgccag cgtttcgctc tctatcacga agcccatttc
      841 agtattgagc aatgttgaac gcggatcgaa attgaaagag ccaataaaca ccgttttacc
      901 atcgatgcta aaggttttag catgcaggct ggcaccggaa ttaccggtta taccgcgatc
      961 gtgtaatgta ctactttgtt cacgcgtcgg cttgagttca tataattcca ccccatagcg
     1021 aagcaatttt ttgcgccagc gcgcgtatcc ggcatggacg acagcaacat cgttagcggc
     1081 aagagaattg gttaggatcg caatctttac cccttttctc accatccgta agagttgcgc
     1141 cacacctgca cgtgtcggta caaaatagga agagataata tcgatgcgtt cactgggtga
     1201 gcccatgata tcgaacaggc gctgcggtaa cagtgaatgc cgttttgcct tgccctcccc
     1261 tttcgccgga tcatcactta ataaacatgt tttcgcccag ataagcggca acgttccatc
     1321 aaccagatga tgtataaacg gactggattc cattttgcgt aagtaacgat gcgtcatgac
     1381 atcgttatgc caggaggcgg gtaactcgat gtgatccgcc atttcacctt ccgggacatc
     1441 cagcacctgc tgtaaggggg aaaccgattt gcaataccag tagcgggcga aatcatcggc
     1501 aacgtcctct accacgggtc ctattgccat gacatctaaa tccgaaaaaa gtggctcttc
     1561 ccctgcccca aaataggcat caccaatatt tcgtcctccc accagcgtca ccacgccatc
     1621 gacagtgaaa cttttattgt gcatacggcg attgagacgg gaaaagtcgg tgatataacc
     1681 aagcggacgt agcaaacgaa acgagaaagg attaaaaagc cggacttcaa tgcgtggatg
     1741 gctgtcaagc aagcgtaaaa tgtcatcaag tccgggcgta ttgttgtcgt ccagcaacaa
     1801 acggacgcga acgccacgct ttgctgcggc taacagggcg gaaaacagta accgacccga
     1861 catatcgtcc tgccagatgt aatactgaac atccagcgta tgctctgcca tttcagccag
     1921 acgataccgg gcggcaaacg catccagact tttctccaga ggaaaaagtc cacactgacc
     1981 gggatgttgc gaacacagtg gcagcaccgc gctcgccagc cgaggcaaat cattcatctc
     2041 cttgttgggt aaggagtctt tcgtagaggt gggcgttttc ttcatcataa cagacaaagt
     2101 atacctgttc gggtaaagcg tgacgggtaa taaattctga aacggttttt actgcgattt
     2161 cagccgctgc cgcacgggga taaccataaa ccccagtact gattgcagga aaagccactg
     2221 acgtatagct gtttgccgcc accagtcgta ggctattgag ataggcatcc tgcaaaagct
     2281 ggtcttcgtt ttgttcacca ccacgccaga ctggcccgac ggtgtgcact acggctttag
     2341 cgggaagatc gcctgcaagc gtaataacgg catgccccgt agggcaatcg ccctgctgct
     2401 gcctgacttt taaacaggca tccagcaggg ccggacccgc tgcgcgatga atggccccat
     2461 cgacgccgcc gcctcccatt aatgacggat tagccgcatt cacaatcaca tcaacggcca
     2521 gtttggtaat atcaccctgc acaacatgaa tacgcgtttt cataacgcct ccttacacct
     2581 tcttgtttcc taagtgtaca gcaggctttt tagttaaatc gcatttttct gcttagcaac
     2641 gtgagttgtt cagaaactta gttatattcg atgttcatta cagccagctt tttctgttta
     2701 tcaagatact gcacttttac tgaggctatt tgcacgtttt taacattgcc gctcattaag
     2761 gctttgctgg aataggtttt acgcggaaca gaaccattct gtgggcagtt catttctacg
     2821 ggtgatgact gggtgctgac gtcacatgcc ggttccacaa tttggccata aaaatggatc
     2881 acgccccctt gcgcggtgct tcctgcaatg gcaataaaag gaaaaaacaa actgccgagc
     2941 ataagagagt ttaaaaaagg tctgaacatt tattctattt cctcaatgat tagtcatcct
     3001 tgagggttgt gttatccata ctttcaggtt ggctgattcg cagcagacca ttctctccag
     3061 gttcatctta tgctcgatat ttcaacaaat taagactttt ctgaagaggg cggccattgt
     3121 tgtgataaat gaagtgactg tccatcagaa acagtaacaa ctattttcac ccgatcgtcc
     3181 ggggaaatat ttaaactcaa cttcgtcaaa gcaatgggtt gattagcagg caatgagagg
     3241 gtcttttctt gcttcgtctg actttgccct gaactgcctt cgcgcaggga caatatttgt
     3301 actctgcaca gacaagattg agtaagagtg acttcaggaa taatggtgta catatcccct
     3361 tgctgggtcg tattaaaggt tatctgactg gaaagtgccg caaggagtaa taacgcattc
     3421 atattcttct cccgaaaaaa aaacagggct tacgccctgt ttctttcata cagatgatgt
     3481 attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtgacg ttaacgctgg
     3541 agttagatgc agtctggtca acagcagcac cgttaccgcc accgaactgt ttaacagtca
     3601 tagtagaatc tttaccattc cactgatcaa gagtagcgct gttgccgaaa ccacgctgag
     3661 tcagatcgat tgagctgtca tcagaacctt ggccaacatc tgcaccatta ccgccgccgt
     3721 gctgggtaat ggtcagatca gagttgcggg cgtcagcttg cagagcaaga gcagagttac
     3781 cgccaccgta ctggtaaata ttcagctctg aattcgggcc gctgttatta ccgccaccac
     3841 cgtggttgcc gccaccgccg ccgtactgag gaacaacacc tgccagagcg ctaccggaga
     3901 atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc catcggattg
     3961 atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc
     4021 tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt attaccagaa
     4081 cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact ggcatcgttg
     4141 gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc aatctttgcc
     4201 cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac
     4261 tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa tgaagactta
     4321 ctcaattcat ttaccgcaaa gttatattct gaattagcta aatcataacc tgctgcggct
     4381 gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt tttcatgttg
     4441 tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg ttgttaacgc
     4501 aacccgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa atatttttaa
     4561 t
//
LOCUS       DAPQZD010000261         4512 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPQZD010000261 DAPQZD010000000
VERSION     DAPQZD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.264"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            12..194
                     /locus_tag="RO454_004772"
     CDS             12..194
                     /locus_tag="RO454_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936482.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDNDATPDEIHDEAKDIVFNYC
                     NYSYHEIKDEEEEQNG"
     gene            187..363
                     /locus_tag="RO454_004773"
     CDS             187..363
                     /locus_tag="RO454_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936483.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            360..676
                     /locus_tag="RO454_004774"
                     /pseudo
     CDS             360..676
                     /locus_tag="RO454_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1285..1440
                     /gene="hokD"
                     /locus_tag="RO454_004775"
     CDS             1285..1440
                     /gene="hokD"
                     /locus_tag="RO454_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDY0936484.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1608..1880
                     /locus_tag="RO454_004776"
     CDS             1608..1880
                     /locus_tag="RO454_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936485.1"
                     /translation="MAHKTKFHNSDTDLAIRHERRSHAFKTDWYQHPPCTEEQAEWLI
                     HSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1882..2940
                     /locus_tag="RO454_004777"
     CDS             1882..2940
                     /locus_tag="RO454_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDY0936486.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMCG
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLCDQTSESLEQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADVLPESVLCRSLGLPVEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPLHVHQQQPPLQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2941..3303
                     /locus_tag="RO454_004778"
     CDS             2941..3303
                     /locus_tag="RO454_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDY0936487.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMH"
     gene            3318..4139
                     /locus_tag="RO454_004779"
     CDS             3318..4139
                     /locus_tag="RO454_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDY0936488.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSVASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYSSYWEEW
                     KKVKEVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            4283..4358
                     /locus_tag="RO454_004780"
     tRNA            4283..4358
                     /locus_tag="RO454_004780"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4316..4318,aa:Ile,seq:cat)
BASE COUNT         1224 a          973 c         1223 g         1092 t
ORIGIN      
        1 gcggtagtta aatgcgaaag tttaaaataa ttattgaaac gggaatagcc ggtggagatt
       61 tcgaggatga attcgaagtg gataatgatg cgacgcctga tgaaatacat gacgaagcaa
      121 aagatattgt ctttaactac tgcaattact catatcacga aataaaagac gaagaggaag
      181 aacaaaatgg ctgattttgg ttcaactaaa tacaacgtca gttttgaaga atggcatgaa
      241 ctgttaatgg actatgcaga gttacgtggt ggcagtgctg ctgatgctga agcatggcgt
      301 gatgattatg aagcaggaaa aactccggtc gaagcatatt gtgatgagtg gggcgatgaa
      361 tgagcgaggt taattatcag gaagggcatg aaacggcggg gcaagcaaaa acagtggcat
      421 ggcgatatcg ctacgtgaaa aaaaaggcgt tacggacttt caggggaagt agtggtctgg
      481 tgactggaaa tatgtaccga aaaaagagga ttgtaacgac aggccgaact atgaaattca
      541 ggccttattc actgccccgc cagtcccggt tacatcagaa gaactggtta aagctgtgca
      601 cttttatgaa caactaaaac gcgaaaatcc accagcatcc ggaaacctga ttacagattc
      661 ccagataagg caatgagcta cctgacgcag aacgggctga taagtatggg gaatgtttta
      721 cgatgaatat ttagactaaa gagtttgtaa cgctatgtaa gtgatttttt ctggtttaga
      781 tatttatatg tccggccaaa ttgaggtgtg tttaaatgta attgcacatt gattgtagga
      841 ggaataatga aaaacgcatt gcagtttttg tttgttgcgt tctggttgtt cgtatcatgt
      901 atgcccatca tcttcacagc aaggtatatg gaaaaagttg atgttttgat attaatattt
      961 ggatatataa atgccctttt tttaggggtg ttcatggcgg tcatgtgcat tgaatactgg
     1021 cggtaaatac agcgaacgcc attggtttag ttggatattt actgtgccgg acaaaaacgg
     1081 tttgcgggga aatcttagtt aagtagaatg actgcgggtg cttgaggcta tctgtctcag
     1141 gcatgaacac caaaaggcag atagagaaaa gccccagtta acattacgcg tcctgcaaga
     1201 cgcttaacat taatctgagg ccatatctat gcgacacata gagattagcc tcttacggac
     1261 cgaaaggtca aggagaagca ggctatgaag cagcaaaagg cgatgttaat cgccctgatc
     1321 gtcatctgtt taaccgtcat agtgacggca ctggtaacga ggaaagacct ctgcgaggta
     1381 cgaatccgaa ccggccagac ggaggtcgct gtcttcacag cttacgaacc tgaggagtaa
     1441 gagacctggc gggggagaaa tccctcgcca cctctgatgt gtcaggcatc ctcaacgcac
     1501 ccgcacttaa cccgcttcgg cgggttttgt tttttcctgg cattctggtt tacaattcgc
     1561 acgtcagcct gaacacctga cacctgctgc gccagcagag aaaacagatg gcgcacaaaa
     1621 ccaaatttca caattctgat accgaccttg ccatccggca tgagcggcgt tcacacgcat
     1681 ttaaaaccga ctggtaccaa cacccaccat gtactgaaga acaggccgaa tggctgattc
     1741 attcttaccg caggcgcggg ttcgaggtta agaaagctct cagtctcgac tatcggcact
     1801 ggataatctc tgtcaggctg ccttattccg aacgcccacc acgtccgtcc cgcactttcc
     1861 agcaacggat ctggaggtaa cgtgcgggta ttacttagac ctgttctggt gcctgagctt
     1921 gggctggtgg tccttaagcc gggccgtgaa tccatacaga tatttcataa tcctcgagtg
     1981 ctggtggagc cggaaccgaa aagcatgtgc ggcctgccat ccggagtcgt ccctgccgtt
     2041 cgccagccgc tggcggagga taaatcatta ctgccatttt tcagcaatga gcgtgtgatt
     2101 cgtgctgctg gcggcgctgg tgcactgtct gactggctgt tgcgccatat taaatcctgc
     2161 cagtggccac acggtgatta tcaccacagt gaaaccgtca ttcaccgtta tggtaccggc
     2221 gcaatggtgt tgtgctggca ctgcgacaac cagttgtgtg accagacctc agaatcactt
     2281 gagcaacttg ctcaccaaaa cttgtcagca tggatgattg acgtcatccg tcacgcaatc
     2341 agcggtacgc aggagaggga gttatcgctg gccgaattat cctggtgggc ggtctgcaat
     2401 caggtggctg atgtgcttcc ggagtctgta ttgtgtcgtt cactgggatt accggtggaa
     2461 aaaatccgct ccgtataccg tgagagtgac atcgtaccgg gagaacagac tgccaccagc
     2521 atactgaagc agcgcacaaa aaatattgcg ctgccacttc acgtccacca gcaacagccc
     2581 ccactccagg aaaagacgtt agtaagcatc gccgttgatc cggagtctcc ggctcagtat
     2641 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
     2701 cagaaatgca tgacgtgcgg taatcaggca gatgatccgc atcacatcat tggtcatggc
     2761 ctgggaggta tgggaacaaa ggctgatgat ttgtttgtta ttccgctgtg ccgtaaatgt
     2821 cataacgaac tgcacgccgg ggtaaaagat tttgaagaaa aacacggcag ccagctgttg
     2881 ttgctgattc gttttttaat gcacgcgaga aattcgggtg tcctgaagtg gaaagcatga
     2941 atgactgaac gcatagaatt tgttttgcct tacccgccga cggtgaatac ctactggcga
     3001 cgtcatggca atacgtattt catctcggag gccggaaagc gttatcgccg tgatgtggcg
     3061 ctaattgttc gccagcagcg gctgaaatta aacctgtccg gaaggctggc gataaaggtg
     3121 attgcagagc caccggataa gcgtcgtcgc gacctggaca atatcctgaa agcaccgctg
     3181 gatgcgctga cgcatgcggg agtgttaatg gacgatgagc agtttgatga aatcaatatc
     3241 gttcgtggtc agccagtatc tggtggacgt ctgggggtga agatttaccc cataatgcat
     3301 taagagcagg tcaaaaaatg aaactggaag atttaccgaa atactactcc ccaaaatccc
     3361 ctggcctgac cgatgcatcg gcctcaacgt caaaagatgc gctgagtatc actgatgtga
     3421 tggccgcgca gggcatgaca cagaatcggg ctgagatggg tttttctgcg ttcctgggga
     3481 aaatgggcat cagtatgaat gacagggcgc gggcaacaga attactggca gattatgcac
     3541 tcagtcggtg cgatcgtgtg gcggcgttga gaaaacttcc ggcagaaata aaaccggtag
     3601 tgatgcgcat tatggcttcg tacgcttttg aggattatgc ccgcagcgta gcgagtaaaa
     3661 agcagtgccc ttgttgctat ggggaaaaat ttattgaaag cgtagttttt acaaacaagg
     3721 tccagtatcc ggatggtaag ccgccggtat gggcaaagtg tacgaaaggt gtgtattcgt
     3781 cttactggga agaatggaaa aaagtcaagg aagtggtaaa agttgcctgt ccggagtgtg
     3841 gcggaaaggg tgaggtttcc accgcctgta aggattgccg tgggcgtggt gtcgccattc
     3901 atcgtgaaga gtcggtaaaa cgtggtatgc ctgttatcag agactgccag cgttgtggtg
     3961 gtcgtggcta tgaaagacta ccatcaacgg aggcatttaa tgctatatgc gaggtgacaa
     4021 accagataac acgcgcgtca tgggaaaaaa cagttaagaa attctatgat gcgctggtga
     4081 cccggtttga tattgaagaa gcatgggctg agcggcagtt aaaaaaggta actaggtaac
     4141 aaggttgatt tttccggaat ctgtggtaaa ttcgtcataa caatgggctt tttatgcctg
     4201 acgttagaag agtttctaca acccgccgcc gagcgggttt tttattgcgg aattaattac
     4261 ggaccgttat tattctgctc ccggcccttt agctcagtgg tgagagcgag cgactcataa
     4321 tcgccaggtc gctggttcaa atccagcaag ggccaccatc acaaaccgcc attagcttat
     4381 caggaagagc agacgacacg ataacagggt tgttggtgcg ggggcgggtc cccgatggcg
     4441 gtccattatc ggtattcagc gttgttagct cagccggaca gagcaattgc cttctaagca
     4501 atcggtcact gg
//
LOCUS       DAPQZD010000262         4470 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPQZD010000262 DAPQZD010000000
VERSION     DAPQZD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.265"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            129..1352
                     /locus_tag="RO454_004781"
     CDS             129..1352
                     /locus_tag="RO454_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDY0936489.1"
                     /translation="MGTIQKIRTDVNVLYAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1337..1981
                     /locus_tag="RO454_004782"
     CDS             1337..1981
                     /locus_tag="RO454_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDY0936490.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
     gene            complement(2352..4469)
                     /locus_tag="RO454_004783"
     CDS             complement(2352..4469)
                     /locus_tag="RO454_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDY0936491.1"
                     /translation="MLSTLSLCISGIVSTATATSSETKISNEETLVVTTNRSASNLWE
                     SPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLILI
                     DGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGDK
                     LASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYRN
                     NSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFYD
                     TDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQA
                     VTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQS
                     TVSLFAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDHE
                     MVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNNF
                     ELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRAK
                     TWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQA
                     NIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDKR
                     YRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT         1160 a          967 c         1107 g         1236 t
ORIGIN      
        1 taaaaactta actatcatga actcgtgata gtaacaatat acccttaagg tagaactttt
       61 tcttgatgag aaggtttaag tttgtagttc agaatattac tgccaagata gtttatacta
      121 ggatatgtat gggaaccata cagaagataa gaacagatgt taatgtatta tatgcggcca
      181 ttcaccgtat tgagtggttg tttgagacat tttcatctgt ttgtttgtct ttttctggag
      241 gaaaagactc tactgtgctg ctccatctta cggccgatgt ggctcgcagg aagaaacgtc
      301 gtttctctgt attattcatt gactgggaag ctcagtacca gtgcacaatt gcacacattc
      361 tgaagatgag ggagatgtat cgggatgtga cggagacttt ttactgggtg gcactccccc
      421 tgactacggt aaacggtgtc tctcagtttc agccggaatg gatatgctgg gaaccaggtg
      481 tggagtgggt tcgtcagcca ccagatgacg ctattacaga tatgtcgtat ttcccatttt
      541 atcggtatgc catgacgttt gaagaatttg ttccggcatt ttcttcctgg tttgccggta
      601 accggtgtgg agtggcaata ctgactggtg ttcgtgctga tgaatcgctc aatcgcttta
      661 tgggactggt gtctcagcgc aaactgagat atgcagatga taaaccctgg accacagcgt
      721 cacctgaagg gttttattac accttgtatc cgttgtatga ctggaaagcc cgtgatatat
      781 ggatatataa cgccagaacc cgagctatct acaatcccct gtatgacctg atgtaccgtg
      841 ccggcgtgcc gttacgcaac atgcgggtct gtgagccttt tggcccggaa cagcgtaagg
      901 gactgtggct ttaccatgtt ctggagccgg aaacttgggc caggatgtgt gagcgggtgt
      961 cgggtgctgc cagcggggcg ctttatgcca atgagagcgg tgcctatttt gccctgcgta
     1021 agcgtatcac gaagccacct catcatacct ggcgtagcta tgcgatgttc ctgctggatg
     1081 tgatgccgga aagaacggca gaacattacc gtaataaaat tgctgtctac ctgcgctggt
     1141 atcagacgcg gggcttcccg gatgacatcc cggatgaaca ggagaatgac ctggggagcc
     1201 gggatatccc gtcttggcga cgtatctgta agacactcat aaagaatgat ttctggtgcc
     1261 ggaccctctc cttcagtccg aacaaacccc ggcactatga acgttatctg cagcgtatga
     1321 aagaaaggag gaaggaatgg gggattctgt gacacctgag gtggaagtat tgagcaacat
     1381 gattcgacag tacttcagtc aggaacgatc tgaagaagag accatacgag cccttaatca
     1441 tctgcgcagg gtgttgcatg aggtcagccc ttttgcacag gagcctgtgg actgtgtgtt
     1501 gtgggtgaaa gcggatgagg tggtggccaa cgattacaat cccaatgtca tgtcttccag
     1561 tgaaaaaaaa ctgcttaaac attcactgga gcaggatgga ttcacgcagc ctgtggtggt
     1621 ttcggaagag aaggagcatt atctggtcgt ggatggtttt catcgtcagt tgctgggcag
     1681 gaaagcggat acgagaaaac gtctgaaggg ttggctgcca gtgacctgta ttaatccgga
     1741 aagaaaggga caggcatcac gtattgccgc aaccatacgg cataaccgtg ccaggggaaa
     1801 acaccagata acgtcaatgt ctgacattgt cagggacctt tcgcgtctgg gctggacaga
     1861 tgagcgtatt ggcacagagt tgggcatgga ccaggatgaa gtgctgaggc tgaaacagat
     1921 tagcgggctg acagagctgt ttcaggaaga gaatttcagc ccttcatgga cggttcgttg
     1981 atattgtaaa atatgatagt tggagtaagg ggcaatagaa ggagaatatt tatcccacct
     2041 tcttccgagt cgggataaat caaaaggtat tatccattga ttcagtatct gggtgagagt
     2101 aaataatcta aaaatagtat tgcgttgtga actgatatca cttttgaatt acaaaggtat
     2161 aggctcagta accagcttta taagtcattg tcgtagcaat gactatagag tcatgctggc
     2221 gggaagaata aatattcacc cccagatgga aaacaacgtc gaaaaacctt caacaaacac
     2281 aatacgttcg ttagcctgcc cgcaaagcgg gcagatcagg acaaagaatt ccataacgat
     2341 tgtttttgtc atcagaaatt ccatacaaaa cctatagctg cattaaaacc tgctgcagga
     2401 atagtttcat gtgctgtacg gtagcgtttg tctgtcaggt tattgagtgc taggttaatc
     2461 cggtactgat cctcgttacc gaattctgta tttactgcaa agttgagagt ggcccagccc
     2521 ggaacatttg tttcggtacc gttactgtca tcttttgcac tagaggcagc acgaataaaa
     2581 acatcagaga ttatgttggc ctgacccatc acaagagtat gtttcagccc tatacgtccg
     2641 tttatcgctg gttctcctgt attagttgtt tttaatgttg aagtttcata ttgccgacga
     2701 attaaattgc cactgatata tggcgagaaa acccagccat tatattccgc gcttatttcc
     2761 agtccccatg tttttgcccg atcaatattg tcataataat agtagctact acgggaggag
     2821 ttggtgttac cattgcaaac tatactgcca tcacagatca gacttgcaat ataatcttta
     2881 gcttctgagt agtaaactgc gctgtcaatc agccactgat taccattata tcgtgcacct
     2941 aattcaaagt tattggagtg ttcagcctta agatcaggat ttccgtatgt gacactgccg
     3001 cccgcagatg tctgcataaa aagctgggag agtgtgggaa atacgtagcc ttgcgcgaac
     3061 gcagcgcgta actccaagtt atcgaaacct gaatagcgca ggcttgtaga tgttaccatt
     3121 tcgtgatcac tcgcagactc tctggcaaga gaggtatcgc ttataatgcc tgcggtatat
     3181 gatactccgt caccacgcgt caactttgaa gaaagccagt attggcgaac tcccattgtc
     3241 catgtccagt gatcggcgaa tcgccagtca ttttgtgcga atagtgagac tgtactttgc
     3301 tctgactcat aataggagcg agttcgtgtt tccttattaa tgaagccggt cagagatttg
     3361 cttgaggtca tgccaccgga cctttggctg accctgtctt gtttgtactg tgcaccggta
     3421 acaagttcat tattagcagg cagcgaaaag tgactctgca atgtgaccgc ctgagtgtat
     3481 tgcttatcat gggtgtcagt cttgttatga acggtcagag cctgaatcat cggactggga
     3541 acaggctgtg tcgtttttac ttcgttggca aattggcgct ggatggtctg ctcatacgcg
     3601 tcgaaatgaa ttttttttag atagtcaccg tccacgtctg tgtcatagaa taccccaact
     3661 ttctctcgtt caagtttagg tattttgaca ctaaatgcct catagcttcc gtctggatcc
     3721 tcatagtaag tttgcgtcgc gagtctgtag cgatcaagcg agaggccaaa acgatggttt
     3781 ccggagttat aacccaacca tacaccctga ctattgttac gatagttggt attcggcaga
     3841 cgtccatccg gcgtatcacg attgccctga tcagaataac taccgttgat gcgataatca
     3901 aatccaccga tgctcccctg gaccgcgatt gattcttccc agcctgctgt tgcggaatta
     3961 taaacagctt tcacaactcc agatgcaagt ttgtcacctc cctttttggt tatgaagtta
     4021 acaataccgc caattgcctg tgaaccgtac agtacggaat atggaccttt cactacctca
     4081 acacgctcca gcgcagactc atctatcaac agtcccacac cataattatc tccggcgcgc
     4141 tgataagtta cctcctgacc atcaatgaga attaaaacac gggaggatgc ttcgccacga
     4201 atgcggattt gtttacggcc tgccaaggag ttgtctgtta tctctactcc ggggatgtcc
     4261 tgcaaattat cggctatgga ggcattggtg gagttctgca atgtttgttg gtcaataacc
     4321 tgtatagtcg ccgggctttc ccaaaggttg cttgccgaac gattcgtggt cacgacgagc
     4381 gtctcttcgt tgctgatttt tgtttctgaa gaagttgcgg ttgcagtaga aactatccca
     4441 gaaatgcata atgacagagt ggaaaggata
//
LOCUS       DAPQZD010000263         4462 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPQZD010000263 DAPQZD010000000
VERSION     DAPQZD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.266"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..379
                     /gene="tnpA"
                     /locus_tag="RO454_004784"
     CDS             <1..379
                     /gene="tnpA"
                     /locus_tag="RO454_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDY0936492.1"
                     /translation="VFYREKRRAIGSILRKLCEWKSVRILEAECCADHIHMLVEIPPK
                     MSVSGFMGYLKGKSSLMLYEQFGDLKFKYRNREFWCRGYYVDTVGKNTAKIQDYIKHQ
                     LEEDKMGEQLSIPYPGSPFTGRK"
     gene            500..2878
                     /locus_tag="RO454_004785"
     CDS             500..2878
                     /locus_tag="RO454_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDY0936493.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPE
                     ALRRFEQMVEEAARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAATSK
                     TASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQVA
                     AAQSESAAAGSATSAAGSATAAANSRKAAKTSETNAKSSQTAAKTSETNAKASETAAK
                     NSQDAAAQSESAAAGSASAAAASATASANSQKAAKTSETNAKTSETAAANSAKASAAS
                     QTAAKASEDAAREYASQAADPYKYVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDD
                     EITMPGDKIVKFKRASKATYINKSGVLTEAAIDEPRFERDGLLIEGQRTNLLLNSTNP
                     SKWNKSGNLELTEISTDSFNFTYGRFTVKDTLIGQTSAINIVTISGSKGFDVTGDEKY
                     VTISCRVRSDVENIRCRLRFEHHDGYTYTFLGDAYLNLSTLVIDKTGTAADRIIAKAV
                     KDEVTGWIFYQATINALDTESMIGAMVQYAPVKGSGTASGDYLDIATPQVEGGSSASS
                     FIVTDITASTRASDMVTVPIKNNLYNLPFTVLCEVHKNWYKTPNAAPRVFDTGGHQTG
                     AAIILGFGRSTDYDGFPYCDIGLANRRVNENASLEKMVMGMRVKSDQSTCSVSNGRIS
                     SEKKATWSYIQNSAIIRIGGQTTAGLRHLFGHVRNFRIWHKALTDAQMGESI"
     gene            2878..3159
                     /locus_tag="RO454_004786"
     CDS             2878..3159
                     /locus_tag="RO454_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936494.1"
                     /translation="MKDLTLKFADRADFSAFMDSIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYYVNVRIINDAKKSSIFDEYAVVVEHQLRGWM"
     gene            3169..3873
                     /locus_tag="RO454_004787"
     CDS             3169..3873
                     /locus_tag="RO454_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDY0936495.1"
                     /translation="MATSTVIPGDITTLKGDVSKAKEDISSINGKVSTLQADMTSAKQ
                     DISTRYTKTEVDNKLKNKLEVNALESGRYGGDFYPLTGREAFYLWGLGTTTAAANLYL
                     NPDPAISSVLRSTSSIRYKHSVETIDSEHADLIFRMRPVWYRSQCENDRRDWGFYGLI
                     AEEVGEIAPQFVHWRPANEDDAPEAISSNGLVAEGVMYERLVVPLIHHIQKLTERVDE
                     LESELKLLSVSRSDIG"
     gene            3884..4171
                     /locus_tag="RO454_004788"
     CDS             3884..4171
                     /locus_tag="RO454_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936496.1"
                     /translation="MDISPLLHALCAVAAQTLVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVTSLMDFAVPVVACLLIWMLIR"
BASE COUNT         1329 a          913 c         1167 g         1053 t
ORIGIN      
        1 ggtgttctac agagagaagc gtagagcaat aggcagtatt ttgagaaagc tgtgtgagtg
       61 gaaaagtgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      121 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      181 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      241 cagagggtac tacgtcgata cggtgggtaa gaacacggcg aagatacagg attacataaa
      301 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctatc cgggcagccc
      361 gtttacgggc cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      421 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      481 tatttattgt ggagtggata tggcagcagt aaaaatctca ggtgtgctga aagatggtgc
      541 gggaaaacca atacagaact gcactattca actgaaggca aagcgtaaca gcaccacggt
      601 actggtgaac acggtggctt ctgaaaatcc ggatgaagcc ggacgttaca gcatggatgt
      661 tgagcatggt cagtacagcg tcaccctgct ggttgaaggt tttccgcctt cacatgccgg
      721 gaccattacc gtctatgaag gttccagacc aggtacgctg aatgattttc tcggtgccat
      781 gacggaggat gatgtccgac cggaggcact gcgccgcttt gagcagatgg tggaagaagc
      841 ggcacgcaac gctgaagccg cctctcagag cgcagcggcg gcaaagaaat ccgaaactgc
      901 agcggcatca tcgaagaacg cggcgaaaac ctcagaaacg aatgcagcta acagcgcaca
      961 ggcggcagcg acctcaaaga ctgcatcggc aaactccgcg acagcagcca aaaaatcaga
     1021 aaccaacgcg aaaaatagcg agacagccgc aaagacgagc gaaaccaacg caaagtccag
     1081 ccagacggca gcgaaaacca gcgaaacgaa tgccaaagcc agtgaaactg cggcaaaaaa
     1141 cagccaggtt gcagcagccc aaagcgagag cgcggcagcc ggttctgcga cttcagcagc
     1201 tggatcagca actgctgcgg ctaacagccg gaaagctgcg aagacgagtg aaactaacgc
     1261 aaagtccagc cagacggcag cgaagaccag cgaaacgaat gccaaagcca gcgaaactgc
     1321 ggcgaaaaac agtcaggatg cagcagccca aagcgagagt gctgcagctg gttctgcaag
     1381 cgcggcggct gcttctgcca ctgcatcagc caacagtcaa aaagcagcaa aaaccagtga
     1441 aaccaatgca aagacaagcg agactgcagc ggcgaactcg gcgaaagcat ccgctgcaag
     1501 ccagaccgct gcaaaagcaa gtgaagacgc agccagagag tatgcaagcc aggcagcaga
     1561 tccgtataaa tatgtcttac agccgctgcc tgatgtgtgg ataccgttta acgattcact
     1621 ggatatgatt acgggctttt cgccatcata taaaaagatt gttattggtg acgacgaaat
     1681 aacgatgcct ggcgacaaga ttgttaagtt taaacgtgca tcgaaagcaa cctatattaa
     1741 caaatctggt gtgctgacag aggctgccat tgatgagcca cgatttgaac gtgatggcct
     1801 gcttattgag gggcaaagaa ctaatcttct gcttaattca acaaatccat ctaaatggaa
     1861 taagtcaggc aatctggaac tcacagaaat atccacggat tcttttaatt ttacttatgg
     1921 gagatttact gtaaaagata ctcttattgg tcagacaagt gctattaata tcgtaacgat
     1981 ttctggcagt aaagggtttg atgtcacagg tgatgaaaaa tatgtgacca tttcatgccg
     2041 tgtaagaagt gatgttgaaa atataaggtg tcgtttaaga tttgaacacc atgatggtta
     2101 tacttacact tttttgggag atgcttacct caatttatca acacttgtaa ttgataaaac
     2161 tggtactgct gcagaccgta ttattgcaaa ggctgtaaaa gatgaggtta ctggttggat
     2221 tttctatcag gctacaatta atgcactaga tacagagagc atgattggtg cgatggttca
     2281 atacgctcct gtaaaaggtt caggtacagc atctggagac tatctggata tcgcaactcc
     2341 acaagtggaa ggtggatcaa gtgcttcgtc atttattgta actgatataa ctgcaagcac
     2401 tcgcgcaagc gatatggtga cagtcccaat caagaataac ctttataatc ttccttttac
     2461 ggttctttgt gaggtacata agaactggta taaaacgcca aatgcagcac cgcgtgtttt
     2521 tgataccggc ggtcatcaaa ccggagcggc tattattctt ggcttcggtc gttcaacaga
     2581 ttacgacgga tttccttatt gtgatatagg tttggctaac agacgggtaa acgaaaacgc
     2641 atcgcttgaa aaaatggtta tggggatgcg tgtaaagtca gatcagtcta cgtgctcagt
     2701 aagtaacggg cgtatatcca gcgaaaagaa agccacatgg tcctatattc agaactccgc
     2761 aattatccgt attggaggcc agactacagc cgggttgcgt catttatttg gtcatgtcag
     2821 gaatttcaga atatggcaca aggcattgac tgatgctcag atgggggagt caatctaatg
     2881 aaagatttaa cactcaaatt tgcagacagg gccgactttt cggcctttat ggatagcatt
     2941 ggctattatg atgacgagtc gatgcaggat gatattctta ttgacgtgat aggtaacgtg
     3001 tacaaagaaa ccggagaact gactgaagat ggcgaaccgg tatgtgttaa ggaagatgga
     3061 tattatgtaa acgtgcgcat cattaatgat gcaaaaaaat cgtcaatatt cgatgaatac
     3121 gcggttgtag ttgaacatca acttcgtggc tggatgtgag ggagacaaat ggctacatcg
     3181 acagtaattc caggagacat caccacgtta aagggagatg tcagtaaagc caaggaagat
     3241 atttcctcaa ttaacggaaa agtatcaacg cttcaggctg atatgaccag tgcaaagcag
     3301 gatatcagca ccagatacac aaaaactgaa gttgataata agctgaaaaa caaactggaa
     3361 gtgaacgctc tggaaagcgg tcgttatggt ggagattttt acccgttgac tggccgtgaa
     3421 gcgttttatt tgtggggatt gggcacgact acagcggcgg caaaccttta tcttaatcct
     3481 gaccccgcaa tttcgtctgt gctgcggtca acatcgtcta tccgctataa acattcagta
     3541 gagacaatag attcagagca cgccgatctc attttcagga tgcgccctgt gtggtacagg
     3601 tcacaatgcg aaaatgacag gcgtgactgg ggattctacg gattgattgc cgaggaagta
     3661 ggagaaattg cccctcagtt tgtacactgg cgaccagcta acgaagatga tgcaccggaa
     3721 gctatttcca gcaatggcct tgttgccgaa ggtgtaatgt acgaacgtct ggttgttcca
     3781 ctgattcacc atatccagaa gctgactgaa agagttgatg aacttgagtc agaattaaag
     3841 ttgttatccg tttcccgaag cgatatcgga taaaggagga gtaatggata taagcccctt
     3901 acttcatgca ctttgtgctg tggctgcgca aacactggtt ggtcttttta ccggaaactg
     3961 ggcttacgga gcgatagccg gttgtacgtt cttcattgcg cgtgaacaca cccaggcaga
     4021 atatcgctgg atcgaaatgt tcgggcatgg caagcgaatg aatatgccgt ggtggggcgg
     4081 ttttgatccg cgcgcgtggg atgtgacaag cctgatggat tttgctgtgc cggtggtggc
     4141 gtgtctgctg atctggatgt tgatccgtta aatataaaag ccgcagtaac ttgccatgat
     4201 aggatactgc ggctggctgg ttaactttcg atagtgcgag tattgaatga tttccagccg
     4261 ttactgattt tatataacgg cagtggaatt attcaacaca caggcaaaac acaggcaaaa
     4321 cggaataagc tgagaaaaaa agcgtgcttt cccaatctgg ggcaaatcac gtctgaatga
     4381 cacctgaaaa caggtggtgg actcaagaag gcttggaata ttcttcttta atgttatgta
     4441 attcattgat ttttcgtgta cg
//
LOCUS       DAPQZD010000264         4311 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPQZD010000264 DAPQZD010000000
VERSION     DAPQZD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.268"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..299)
                     /gene="tuf"
                     /locus_tag="RO454_004789"
     CDS             complement(<1..299)
                     /gene="tuf"
                     /locus_tag="RO454_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDY0936497.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(414..489)
                     /locus_tag="RO454_004790"
     tRNA            complement(414..489)
                     /locus_tag="RO454_004790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:ggt)
     gene            complement(496..570)
                     /locus_tag="RO454_004791"
     tRNA            complement(496..570)
                     /locus_tag="RO454_004791"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Gly,seq:tcc)
     gene            complement(687..771)
                     /locus_tag="RO454_004792"
     tRNA            complement(687..771)
                     /locus_tag="RO454_004792"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Tyr,seq:gta)
     gene            complement(780..855)
                     /locus_tag="RO454_004793"
     tRNA            complement(780..855)
                     /locus_tag="RO454_004793"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Thr,seq:tgt)
     gene            1217..2167
                     /gene="coaA"
                     /locus_tag="RO454_004794"
                     /gene_synonym="panK"
     CDS             1217..2167
                     /gene="coaA"
                     /locus_tag="RO454_004794"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDY0936498.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2196..3161)
                     /gene="birA"
                     /locus_tag="RO454_004795"
     CDS             complement(2196..3161)
                     /gene="birA"
                     /locus_tag="RO454_004795"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDY0936499.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3158..4186)
                     /gene="murB"
                     /locus_tag="RO454_004796"
     CDS             complement(3158..4186)
                     /gene="murB"
                     /locus_tag="RO454_004796"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDY0936500.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGRAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1115 a         1065 c          968 g         1163 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcgcgagc agcaccgccg
      181 taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc atggtcaacg
      241 tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc tttagacatc
      301 gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca ggcaacatgc
      361 ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag aagtggtgct
      421 gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta ccaactgagc
      481 tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      541 ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct gattttcatt
      601 ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac ttatcgtctc
      661 gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc
      721 tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac cggacttgat
      781 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
      841 ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt tcatgcaact
      901 aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga agaaacagcc
      961 gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat gtgctcctgc
     1021 taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca acgatagctt
     1081 cctgggagag attttttctt attattcctc cccatctggt gttaccctcc tgcccattaa
     1141 cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata gagctatgac
     1201 cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt acctacagtt
     1261 tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat cggaagatga
     1321 gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg ccgagatcta
     1381 tttacctctg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc gtcaggcagt
     1441 tttggaacag tttcttggta ccaacgggca acgcattcct tacattatca gtattgctgg
     1501 tagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat taagccgttg
     1561 gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc ctaatcaggt
     1621 tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg atatgcatcg
     1681 cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag cccctgttta
     1741 ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc agcctgatat
     1801 tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac acgatccaca
     1861 tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac cggaagactt
     1921 acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt ttacagaccc
     1981 ggattcctat tttcataact acgcgaaatt aactaaagaa gaagctatta agactgccat
     2041 gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac ctactcgcga
     2101 gcgtgccagt ttgatcctga cgaaaagtgc taatcatgca gtagaagagg tcagactacg
     2161 caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact acgcagggat
     2221 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agcaccctgt
     2281 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
     2341 cgattaataa aattatccaa cttttcccag cgcgaaagat aaggtgccaa tccttcttgc
     2401 tcgaagagtt ccagcgccgc gcgtaattca cgtatcagca tggccgcaag actattacga
     2461 tcgagattga tcccggcttc ctgcagcgtg atccaccctt gattaacgac actctcttca
     2521 acacgacgca ttgccatgtt gatccctgcg ccaatgacta tttgcgccgc atcgccggtt
     2581 ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
     2641 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
     2701 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
     2761 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
     2821 gcctgctggt attctgcaac acaggcatcg cccgatttaa gctctccgat acgatcaaga
     2881 aggtactgat tcgtggagtc aataacgggc agcacagtta cactaccgcc atccagctga
     2941 cccaatatct gttcagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
     3001 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
     3061 gcccggctca ttcctaacga ttcacccaac tgctcaccag agtgaaattc gccattcgct
     3121 aacagcgcga ttaatttcag cggaacggta ttatccttca tgaaatcgtc tccactgcgc
     3181 taacttcacc tgatgcacca ataaagcgaa cttcaggctc aagccagaca ttaaattttt
     3241 cacccacttt ttgtcttaca tgaagtgcca gttgcaccac atcttcgctt tttgcattgt
     3301 cttcattaat gaggactaac gcctgttgac ggtgcaccgc agccctaccc atttgcatcc
     3361 cttttagctg gcactgatcg ataagccaac ctgctgccag ttttactgaa ccatccgcct
     3421 ggggataatt tggtgctgtt ggaaattgtg ccagtaatac tttagccgtt tcggcagata
     3481 caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca gggagtttgg
     3541 tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt gtaggatcca
     3601 gacgagttaa gtcaccatac gtcagtacag gttgccactc ttttggcaga cgcaggccta
     3661 cggctacaat agcgaagcga tcctggtatt catgtttaaa aatactgtcg cgatagccaa
     3721 aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca acacaatcaa
     3781 cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg ataggtgatg
     3841 agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct tcctgcaaag
     3901 tgtattttac aaggcgatgc cagttttctc cggctcctac atgtaaatac cacgcatcag
     3961 gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg cgatagtctt
     4021 ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct tctgcggttg
     4081 catgctgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg tgctgagcat
     4141 tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct gctttcctga
     4201 tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat atgacgacag
     4261 gaagagttta tagaaacgca aaaggcccag tcttccgact gggcctttcg c
//
LOCUS       DAPQZD010000265         4177 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPQZD010000265 DAPQZD010000000
VERSION     DAPQZD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.270"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..631)
                     /locus_tag="RO454_004797"
     CDS             complement(<1..631)
                     /locus_tag="RO454_004797"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDY0936501.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEA"
     gene            complement(665..1582)
                     /locus_tag="RO454_004798"
     CDS             complement(665..1582)
                     /locus_tag="RO454_004798"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDY0936502.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLFEEHDRNKKMKII"
     gene            1734..1871
                     /locus_tag="RO454_004799"
     CDS             1734..1871
                     /locus_tag="RO454_004799"
                     /inference="COORDINATES: protein motif:HMM:NF024407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDY0936503.1"
                     /translation="MQCPVTILCVTLCDYLAPELITHSPEESDTVTLRKRRLPLEMMA
                     W"
     gene            2243..2668
                     /locus_tag="RO454_004800"
     CDS             2243..2668
                     /locus_tag="RO454_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDY0936504.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            2665..3015
                     /gene="tnpB"
                     /locus_tag="RO454_004801"
     CDS             2665..3015
                     /gene="tnpB"
                     /locus_tag="RO454_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDY0936505.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            3046..4143
                     /locus_tag="RO454_004802"
                     /pseudo
     CDS             3046..4143
                     /locus_tag="RO454_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1113 a         1068 c          907 g         1089 t
ORIGIN      
        1 ctgcttcctt atatccctgt ctcgctaatt caaaaatata tttggtgcta tatcctgcaa
       61 gtttattttt tgctgccccc atgatggccc gaccagatga aaccgcttca acgcatcccc
      121 gacgaccaca tccacattct gtatccgtta ctccggacaa tatatgccca acatgtccgg
      181 ctaatccaga aacacctgtt aataattttc tgttaactac aattccccct ccaacccccg
      241 tagaaacggt tataaaaacc atatcacaaa tttctttcgg caaaactgta tattctgccc
      301 atgcggctgc ctgggcatca tttatcacac ttccatttaa accagttata tcttccatta
      361 tatttttcag aggatactct ttcagccctc caagattgtc tggattcaga gctgtcaata
      421 ctccattatt aataatccct gttgatgcta ctgcaaatcg ctccgctgtt cccttcattt
      481 cagaaaccag tagacgtaaa gcccctgtca tttcgtcagg acatctttca tgaggtgttg
      541 atatctgttt tttcagcaaa aatgaaccgt catcagatat caacgccgct gaaattttcg
      601 ttcctcctat atcaacagcc agtgttatca ttcattttca cctatataac aatcttgcaa
      661 ttattcagat tattttcatt tttttgtttc tgtcatgttc ttcgaacagt cgctctgcaa
      721 gttttgataa tacatccaga ttcttttcct ttaccggaga aaaaggactt ctgcatacag
      781 gagtatttat aatccccata tagtaaagaa gagtttttat acctgccagg acaccactct
      841 gaataagagt gtcaataacc tggttacagg ctacttgcat ttccttcgcc ttaatcacat
      901 cattgttttt gactgcttca aatatttcaa gataacgcca ccccattata ttatatgtac
      961 taccgattcc cccatctgca cctgcaatta accctgatgc aaatatttca tcgtaaccat
     1021 tatataacac aagctctgga tgattccttt tgatttgttc catctggaat aaatcaccgg
     1081 atgtctgttt taatgcacaa accctgggta ttgtgacaag ctcatttatc tgatctaaag
     1141 aaaaacgcac tccgctaaga gccggtatat tataaactac catcggaagt ccatctgcag
     1201 aatcaataat cttcctgtaa tggatacaat gctcttcaaa tgaaaaagga taataaaatg
     1261 gagtaacagc agatatagca tgatatccgt atttttttgc agcttttgca agtacctcgc
     1321 tttctgccgt actgattccg cccacatgcg ctataagtgt cagtcttcca tctgattcat
     1381 cggctactgt ttcaagaatt ttttctcgct ctgcaacatt ctgaagaaat gcttctccag
     1441 tagagcctcc aacatacaat cccttaatat tttgttcgat attaaaacgg atcagttttc
     1501 gaagactctc catatcgatt tgctgggatt ggtcataagg agtgggtaat gctgatatga
     1561 cccctttaaa ttcacattgc atttttaatc accttcactt aactgcataa tgggatacct
     1621 agtacaccat caagtacaaa gattatccaa cagaaaagaa gcacaatgtg aagattatca
     1681 aaaaaaaacc atcagaaagc tgtcatgccg ctcagttaag gatcggttga tcgatccagt
     1741 gtccagttac tattctctgc gtaaccctct gcgactatct tgccccggaa ctgattaccc
     1801 acagccctga agagtccgac actgtcacgc tacgcaaacg gcgtcttcct ctggaaatga
     1861 tggcctggtg aattatcagt atggcccatg agcgtaagga accttcacca gattgtgaac
     1921 cggctggaca catgctgcca ggtagtcgcc cctttgtcgc gccgtgatcc atgcccagca
     1981 gggccaggaa gcggggccgt acgccacgtg ttcacgtaaa cagcgcagcc ctgacataac
     2041 accacgctgt atccgctctg gtgcaatatg aacctgctga ccatcgatgg cgtgttctaa
     2101 ctaacacagg acacgccaga acacgacata aagcttcact cgccagatac atggtaagcg
     2161 taaactgacc gccgtatgta gccattaagc cagtattggt aacgtaggtg cccacctttt
     2221 catcaactag tggacacctg ttatggaaca gaaaatatta tccgcagaac cccgcagaac
     2281 attttcaaat gagtttaaac ttcaaatggt taaactggct tcacaactag gagcctctgt
     2341 tgcccgtatt gccagggaac acgatatcaa tgataacctc ctgttcaaat ggctcaggct
     2401 ctggcagaac gaagggcgta tatcgcggcg tcttccggta acaacctctt ctgacgctgg
     2461 cgttgaatta ttacctgttg agataacgcc tgatgagcag aaagagccta tggcggctct
     2521 tactccgctt ttatccactc cttcacaaag cacagtcagt gccagttcct gtaaagttga
     2581 gttccgccac ggtaacatga cgctggaaaa tccttcacca gagctgctca cggtgttgat
     2641 ccgtgaactg accgggaggg ggcgatgatc tcgctcccat cagacactcg aatctcgctg
     2701 gttgccggcg ttactgatat gcgtaaatcc ttcaacggtc tgggtgaaca ggtacaacat
     2761 gtgctggatg agaacccctt ctccggtcac ctgttcatct tccgtggccg acggagtgac
     2821 atgattaaaa tcctctgggc tgatgctgat ggtctgtgcc tgttcaccaa acgcctggag
     2881 gaaggcctgt ttatctggcc tgcggtgcgt gacggtaagg tatccattac ccgctcgcag
     2941 ctggcaatgc tcctcgataa gctggactgg cgtcagccaa aaacatcccg tcttaacgca
     3001 ctgacaatgt tgtaaaaatg tcatggccgg attataaaaa cggtcatgag tcggaaatac
     3061 ctcattcgta tcactgagct ggaaaggctg ctctctgagc aggctgaagc cctccgtcag
     3121 agagatctgc aactgagtct ggttgaagag acggaggcct tcctgcgctc tgcactggca
     3181 cgtgccgaag aaaagatcga agaggaggag cgggaaatag aatatctgag ggcccagata
     3241 gaaaaactgc gccggatgct gttcggtacc cgttctgaaa aactgcagcg tgaggttgaa
     3301 caggctgagg cccaactgaa acaacgcgaa caggaaagcg atcgttacag tgggcgtgag
     3361 gatgacccgc aggttccccg ccagttgcga cagtcacgtc atcgtcgtcc gttaccagca
     3421 catcttcccc gtgaaataca ccgcctggag cctgaagaaa gttgttgccc ggagtgtggc
     3481 agtgagctgg attatctggg tgaagtcagt gctgagcagc tggaactggt gagtagcgcc
     3541 ctgaaagtga tccgcactgt tcgggtaaaa aaagcctgta caaaatgtga ctgtatagtt
     3601 gaagcgccag cgccgtcccg cccgatagcg cgcggcatcg cgggctccgg attacttgcc
     3661 cgcgtgttaa cgggaaaata ctgcgaacac ctgccactgt atcgtcagag tgaaatcttt
     3721 gcccgacagg gtgccgaact gagccgtgca ttaatctcca actgggttga cgcgtgctgc
     3781 cagttaatga ctccgctaaa tgatgccctg taccgttacg tgatgaacac ccgcaaggtt
     3841 cacactgacg acacaccggt aaaagtgctg acaccgggca gaaaaaaggc aaaaacagga
     3901 cgcatctgga cgtatgtccg ggatgaccgg aatgcgggct catcagagcc accggcggtc
     3961 tggttcgcct actcaccaga caggcaggga aaacatccgg tacaacacct tcgtcccttc
     4021 cggggtatcc tgcaggcgga tgcattcagc ggttacgatc ggctgttcag tgccaaacgt
     4081 gaaggtgatg cacagacaga agttgcgtgc tgggtccatg cccggcgaaa aatacacgat
     4141 gtatgaacga catctcttct gacgacattt tcctgct
//
LOCUS       DAPQZD010000266         4084 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPQZD010000266 DAPQZD010000000
VERSION     DAPQZD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.272"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            149..1252
                     /gene="proB"
                     /locus_tag="RO454_004803"
     CDS             149..1252
                     /gene="proB"
                     /locus_tag="RO454_004803"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDY0936506.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1264..2517
                     /gene="proA"
                     /locus_tag="RO454_004804"
     CDS             1264..2517
                     /gene="proA"
                     /locus_tag="RO454_004804"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDY0936507.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2632..2707
                     /locus_tag="RO454_004805"
     tRNA            2632..2707
                     /locus_tag="RO454_004805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2665..2667,aa:Thr,seq:cgt)
     gene            complement(2722..2925)
                     /locus_tag="RO454_004806"
                     /pseudo
     CDS             complement(2722..2925)
                     /locus_tag="RO454_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3071..3541)
                     /locus_tag="RO454_004807"
     CDS             complement(3071..3541)
                     /locus_tag="RO454_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936508.1"
                     /translation="MATILISDCDLLDLLSAPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLIESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKTEQTLTGLLCELVWNFTEGLKSPRWLRTADGVKFISEVAA"
     gene            complement(3519..3704)
                     /locus_tag="RO454_004808"
     CDS             complement(3519..3704)
                     /locus_tag="RO454_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDY0936509.1"
                     /translation="MCRAFGYTSMAGRAGASQDAPVSCNAGKANPVRFRPHEISLSSG
                     GHNKPLQETPYGYNPHL"
BASE COUNT          951 a         1020 c         1180 g          933 t
ORIGIN      
        1 aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa acgccatgct
       61 ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt
      121 ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg tggtaaaact
      181 cggcaccagt gtgctaacag gcggatcgcg ccgcctgaac cgtgcccata tcgttgaact
      241 tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg tgacgtcggg
      301 tgcgattgcc gccggacgtg agcacctggg gtacccggaa ttgccagcga ctatcgcttc
      361 aaaacagctg ctggcggcgg tagggcagag tcgactgatt caactgtggg aacagctgtt
      421 ttcaatttac ggcattcatg tcgggcagat gttgctgacc cgtgctgata tggaagatcg
      481 tgaacgcttc ctgaacgccc gcgataccct gcgtgcgttg ctcgataaca atatcgttcc
      541 ggtaattaat gagaacgatg ctgtcgctac ggcagagatt aaggtcggtg ataacgataa
      601 cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc tgaccgatca
      661 aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga ttaaagatgt
      721 gtacggcatt gatgacgcac tgcgcgcgat tgccggtgat agcgtttcag gcctcggaac
      781 tggcggtatg agcaccaaat tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac
      841 cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag gcatttccgt
      901 cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg
      961 tgcgccgcct gcgggtgaaa tcacggtaga tgaaggggca actgccgcca ttctggaacg
     1021 cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct cgcgtggtga
     1081 agtcatccgt atttgcaacc tcgaaggtcg cgatatcgcc cacggcgtca gtcgttacaa
     1141 cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg caatactggg
     1201 atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt aaggagctgg
     1261 ccgatgctgg aacaaatggg cattgccgcg aagcaagcct cgtataaatt agcgcaactc
     1321 tccagccgcg aaaaaaatcg tgtgctggaa aaaatcgccg atgaactgga agcacaaagc
     1381 gaaagtatcc tcaacgctaa cgcgcaggat gttgcagacg cgcgtgccaa tggccttagc
     1441 gaagcgatgc ttgaccgtct gacactgacg cccgcacggc tgaaaggcat tgccgatgat
     1501 gtgcgccagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg cggcgtactg
     1561 gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat
     1621 gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac aggtaacgca
     1681 gtgatcctgc gcggcggcaa agaaacgtgt cgcactaacg ctgcaacggt ggcggtgatt
     1741 caggacgccc tgaactcttg tggcttaccg gcgggtgccg tgcaggcgat tgataatcct
     1801 gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat gctgatcccg
     1861 cgcggcgggg ctggtttgca taaactgtgc cgcgagcagt cgacaatccc ggtgatcaca
     1921 ggcggtatag gcgtatgcca tatttacgtt gatgaaagcg cagagatcgc tgaagccctg
     1981 aaagtaattg tcaacgcgaa aactcagcgt ccgagcacgt gtaatacggt agaaacgttg
     2041 ctggtgaata aaaacatcgc tgatagcttc ctgcccgcat taagcaaaca aatggcggaa
     2101 agcggcgtga cgttacacgc tgatacagcc gcgctggcgc agttgcaggc aggccccgcg
     2161 aaggtggtgg ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt agatttgaac
     2221 gtcaaaatcg ttagcgatct ggatgacgcc attgcccata ttcgtgaaca cggcacgcaa
     2281 cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg
     2341 gattcctccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg ccagtttgga
     2401 ctgggggcgg aagtggcggt aagcacacaa aaactccacg cgcgcggccc aatggggctg
     2461 gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg tgcgtaaata
     2521 aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca tcgcccggtt
     2581 agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag ggccgatata
     2641 gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac tcctattatc
     2701 ggcaccatta aaatcaagta gttaccccat atttaaatac accacgttct cctcttgtgc
     2761 cgtatttgtg ccattgcgac ttataatcgc atcgattttg ctcgcgtgcc cggtgaaatg
     2821 cctggctgaa aggtgagcct aacgacgaac catttcaaga gtttcccatc ctcccatctc
     2881 tttaagtgca agaagagaga ctccggactg aaccagccag cttgcctttg ctatcaaagt
     2941 accttgagga ccagatgatt tctggcgata tttacagcgc ttccgcaatg agtttttctc
     3001 tgtgccgcca gaggcgttcc ccgctttttc agcaccgcaa taatgtcggc ggaatgtcag
     3061 tcctgttgca tcatgcggcc acctcactga taaattttac gccgtcggcg gtacgcagcc
     3121 agcgcgggga tttgagtcct tccgtgaaat tccagaccag ctcacacaga agcccggtca
     3181 gggtttgttc cgtttttggc tctgctggac gttccagcag tagctggagc agcgcgaggc
     3241 agtattcgca caaggaatct ggttcggaaa taaagtgctc tggtgtggcg ggaagggtat
     3301 caacggtcag gctttcaatc agatgcggcg ggatggggtc gtgcagcgtg ggttgcaata
     3361 gcgcaagagc cgcgtgaagc cgtccgcaga gcgccagccg cagcgccgga tcgtcggttt
     3421 ctatcagggt ttcggcgaag cgggcgcagt agtcggccag gacggtgaaa tccgtggtgg
     3481 cattgaaggg agcggacaat agatcgagta aatcgcagtc agagatgagg attgtagcca
     3541 tagggcgtct cctgtaacgg tttattatgt ccgccactag agaggctaat ctcatggggg
     3601 cggaaccgga cggggttagc cttaccggcg ttacaggaaa ccggcgcgtc ttgcgacgcc
     3661 cccgcccggc ccgccattga ggtgtagccg aacgcacgac acaaaaaaga cgctggtgcg
     3721 tcaagtgtcg cctgtaacgg ttttcaggag gctaatcccg acgcctgatt ttgcaggcgc
     3781 gggagaaaga tactgcgatc ggttatgaac ggcaagcggt tttgctgcgg tgagtgcggc
     3841 gcaatgcgga tctggaatgc gcctcgcaaa accacgccgg ggattatttg accgtgacct
     3901 gtccgttcat aagtagaggg aggagccagt cgcggagttg gattaaatga ctattctctt
     3961 gagtgtttga tgccagttta ttataaattg gcatcatttt attctcgaat aacgccacaa
     4021 catcaggagt ggggcatatg atttcacaat tagtgatatg cgtattggtc aggttattaa
     4081 tatt
//
LOCUS       DAPQZD010000267         4082 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPQZD010000267 DAPQZD010000000
VERSION     DAPQZD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.273"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(46..747)
                     /locus_tag="RO454_004809"
     CDS             complement(46..747)
                     /locus_tag="RO454_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDY0936510.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPK
                     LQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1534)
                     /locus_tag="RO454_004810"
     CDS             complement(1280..1534)
                     /locus_tag="RO454_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDY0936511.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1713..3872
                     /locus_tag="RO454_004811"
     CDS             1713..3872
                     /locus_tag="RO454_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDY0936512.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1075 a         1030 c          986 g          991 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
       61 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
      181 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
      301 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
      361 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
      421 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
      481 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
      541 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
      661 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
      721 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
      781 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
      841 atattacctt aaatcatatt gcctttacct taaattcccc cacctaaaca ccttaaatca
      901 tattaccttt accttaaatc acattaccta tagcattgta acattttgtt ttatataagg
      961 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     1081 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     1261 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     1321 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     1381 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     1441 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     1501 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     1561 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggaaaatc
     1621 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     1681 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     1741 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     1801 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     1861 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct
     1921 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     1981 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     2041 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     2101 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     2161 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     2221 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     2281 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     2341 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     2401 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     2461 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     2521 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     2581 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     2641 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2701 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     2761 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     2821 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     2881 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     2941 agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc aggaaaaatg
     3001 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     3061 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     3121 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
     3181 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
     3241 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
     3301 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     3361 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     3421 gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     3481 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     3541 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     3601 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     3661 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     3721 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     3781 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     3841 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     3901 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     3961 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     4021 gctgcattag agtgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     4081 ac
//
LOCUS       DAPQZD010000268         4056 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPQZD010000268 DAPQZD010000000
VERSION     DAPQZD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.274"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..290
                     /locus_tag="RO454_004812"
     CDS             <1..290
                     /locus_tag="RO454_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0936513.1"
                     /translation="ENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFD
                     SLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGVYEKCLV"
     gene            546..1421
                     /locus_tag="RO454_004813"
     CDS             546..1421
                     /locus_tag="RO454_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HDY0936514.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1468..1800)
                     /locus_tag="RO454_004814"
     CDS             complement(1468..1800)
                     /locus_tag="RO454_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDY0936515.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            1814..4056
                     /locus_tag="RO454_004815"
                     /pseudo
     CDS             1814..4056
                     /locus_tag="RO454_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
BASE COUNT         1027 a          983 c         1073 g          973 t
ORIGIN      
        1 ctgaaaacta tatcaaagaa gccaaatacg acatggcggt gggtcatctc ttgctaaagt
       61 cattttgggc gaatgaagcc gtgtttcaaa tgatgatgct ttcatataac ctatttttgt
      121 tgttcaagtt tgattccttg gactcttcag aatacagaca gcaaataaag acctttcgtt
      181 tgaagtatgt atttcttgca gcaaaaataa tcaaaaccgc aagatatgta atcatgaagt
      241 tgtcggaaaa ctatccgtac aagggagtgt atgaaaaatg tctggtataa taagaatatc
      301 atcaataaaa ttgagtgttg ctctgtggat aacttgcaga gtttattaag tatcattgca
      361 gcaaagatga aatcaatgat ttatcaaaaa tgattgaaag gtggttgtaa ataatgttac
      421 aatgtgtgag aagcagtcta aattcttcgt gaaatagtga tttttgaagc taataaaaaa
      481 cacacgtgga atttagggac tattcatgtt gttgttattt cgtatcttcc agaataagga
      541 atcccatggt taaaaaatca ctgcgccagt tcacgctgat ggcgacggca accgtcacgc
      601 tgttgttagg aagtgtgccg ctgtatgcgc aaacggcgga cgtacagcaa aaacttgccg
      661 aattagagcg gcagtcggga ggcagactgg gtgtggcatt gattaacaca gcagataatt
      721 cgcaaatact ttatcgtgct gatgagcgct ttgcgatgtg cagcaccagt aaagtgatgg
      781 ccgcggccgc ggtgctgaag aaaagtgaaa gcgaaccgaa tctgttaaat cagcgagttg
      841 agatcaaaaa atctgacctt gttaactata atccgattgc ggaaaagcac gtcaatggga
      901 cgatgtcact ggctgagctt agcgcggccg cgctacagta cagcgataac gtggcgatga
      961 ataagctgat tgctcacgtt ggcggcccgg ctagcgtcac cgcgttcgcc cgacagctgg
     1021 gagacgaaac gttccgtctc gaccgtaccg agccgacgtt aaacaccgcc attccgggcg
     1081 atccgcgtga taccacttca cctcgggcaa tggcgcaaac tctgcggaat ctgacgctgg
     1141 gtaaagcatt gggcgacagc caacgggcgc agctggtgac atggatgaaa ggcaatacca
     1201 ccggtgcagc gagcattcag gctggactgc ctgcttcctg ggttgtgggg gataaaaccg
     1261 gcagcggtgg ctatggcacc accaacgata tcgcggtgat ctggccaaaa gatcgtgcgc
     1321 cgctgattct ggtcacttac ttcacccagc ctcaacctaa ggcagaaagc cgtcgcgatg
     1381 tattagcgtc ggcggctaaa atcgtcaccg acggtttgta atagcggaaa cggaatgggg
     1441 aaactcattc cgtttttgtt tatcgcctta gacggcaaaa gtgctgtcgc ccacctgcgc
     1501 ttgcgcatac caggccataa gctccgtggt tcctggttct ccttccgctg gagcccagtg
     1561 cgcatagtca tcggcagcca cgggttgata gccaccgtgt tttacttcaa aaattatgcc
     1621 accggtatcc agcgacagca cggcatgcca ggttcctgcg gccatctcca gcaccgtaca
     1681 ggtttccccc aatatcgccc gatgggtgac ggtaccccga tcgtcaaaat tcagcaccac
     1741 gaaacgaccc cttaatggca acagtagctc gaaggtgtga gggtgtcggt gcgggcgcac
     1801 gtaggtccca ggtcatattc cttccggcgt ccggcatttt accgccagac agctcgggat
     1861 tcgtgatatc accgttcttg cagaatacgg tcagagggaa aatacccgcc gtgagcatgc
     1921 agcgctgata cgtcagcact atcagtatcg tgaatttgcc tggccctgga catttcgcct
     1981 tacccgtctt ttatataccc ggagctggat aagcaacgaa cgtcctggcc tgcttttcga
     2041 tctggcgaca gggtggctta tgcaacatcg tattattctc cccggagcca ctacgctgac
     2101 ccggttgatt tcagaggtaa gggaaaaggc gacgttgcgc ctgtggaaca aactggcact
     2161 gataccgtca gccgaacagc gttcacagct ggagatgctg ctggggccaa ctgattgcag
     2221 ccgcctgtct ttactggaat cactgaaaaa gggccctgtg accatcagtg gtccggcgtt
     2281 taatgaagca attgaacgct ggaaaactct gaacgatttt ggcctgcatg ctgaaaacct
     2341 gagtacactc ccggctgtgc gcctgaaaaa tctcgcacgt tatgctggta tgacttcggt
     2401 gttcaatatt gccaggatgt caccgcagaa aaggatggcg gttctggttg cctttgtcct
     2461 tgcatgggaa acgctggcgc tggatgatgc attggacgtt ctggacgcca tgctggccgt
     2521 tatcatccgt gacgccagaa agattgggca gaaaaaacgg ctccgctcgc tgaaggatct
     2581 ggataaatct gcattggcgc tcgccagcgc atgttcgtac ctgctgaaag aagaaacacc
     2641 ggacgaatcg attcgtgctg aggtgttcag ctacatccca aggcaaaagc tggctgaaat
     2701 catcacgctt gtccgtgaaa ttgcccggcc ctcagacgat aattttcatg aagaaatggt
     2761 ggagcagtac gggcgcgttc gtcgtttcct gccccatctg ctgaataccg ttaaattttc
     2821 atccgcacct gccggggtta ccactctgaa tgcctgtgac tacctcagcc gggagttcag
     2881 ctcacggcgg cagttttttg acgacgcacc aacggaaatt atcagtcggt catggaaacg
     2941 gctggtgatt aacaaggaaa aacatatcac ccgcagggga tacacgctct gctttctcag
     3001 taaactgcag gatagtctga ggcggaggga tgtctacgtt accggcagta accggtgggg
     3061 agatcctcgt gcaagattac tacagggtgc tgactggcag gcaaaccgga ttaaggttta
     3121 tcgttctttg gggcacccga cagacccgca ggaagcaata aaatctctgg gtcatcagct
     3181 tgatagtcgt tacagacagg ttgctgcacg tctttgcgaa aatgaggctg tcgaactcga
     3241 tgtttctggc ccgaagcccc ggttgacaat ttctcccctc gccagtcttg atgagccgga
     3301 cagtctgaaa cgactgagca aaatgatcag tgatctactc cctccggtgg atttaacgga
     3361 gttgctgctc gaaattaacg cccataccgg atttgctgat gagtttttcc atgctagtga
     3421 agccagtgcc agagttgatg atctgcccgt cagcatcagc gccgtgctga tggctgaagc
     3481 ctgcaatatc ggtctggaac cactgatcag atcaaatgtt cctgcactga cccgacaccg
     3541 gctgaactgg acaaaagcga actatctgcg ggctgaaact atcaccagcg ctaatgccag
     3601 actggttgat tttcaggcaa cgctgccact ggcacagata tggggtggag gagaagtggc
     3661 atctgcagat ggaatgcgct ttgttacgcc agtcagaaca atcaatgccg gaccgaaccg
     3721 caaatacttt ggtaataaca gagggatcac ctggtacaac tttgtgtccg atcagtattc
     3781 cggctttcat ggcatcgtta taccggggac gctgagggac tctatctttg tgctggaagg
     3841 tcttctggaa caggagaccg ggctgaatcc aaccgaaatt atgaccgata cagcaggtgc
     3901 cagcgaactt gtctttggcc ttttctggct gctgggatac cagttttctc cacgcctggc
     3961 tgatgccggt gcttcggttt tctggcgaat ggaccatgat gccgactatg gcgtgctgaa
     4021 tgatattgcc agagggcaat cagatccccg aaaaat
//
LOCUS       DAPQZD010000269         4041 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPQZD010000269 DAPQZD010000000
VERSION     DAPQZD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.275"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(24..352)
                     /locus_tag="RO454_004816"
                     /pseudo
     CDS             complement(24..352)
                     /locus_tag="RO454_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="involved in the synthesis of a cofactor required
                     for carnitine dehydratase and carnitine racemase
                     activities; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
     gene            360..602
                     /locus_tag="RO454_004817"
                     /pseudo
     CDS             360..602
                     /locus_tag="RO454_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(774..1100)
                     /locus_tag="RO454_004818"
     CDS             complement(774..1100)
                     /locus_tag="RO454_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDY0936516.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(1108..1293)
                     /locus_tag="RO454_004819"
     CDS             complement(1108..1293)
                     /locus_tag="RO454_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDY0936517.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1290..3929)
                     /locus_tag="RO454_004820"
     CDS             complement(1290..3929)
                     /locus_tag="RO454_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDY0936518.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
BASE COUNT          999 a         1078 c          913 g         1051 t
ORIGIN      
        1 agggaaaaat aattatataa ctgttactgt ttcggtcgca gattctcatc aaacaccagg
       61 cgtttcctgc caggttcaac ttcccgcaat ggctcgactt gatgcaacgt ctgcttacaa
      121 cgagtcacca gcacctgatg ccctgctttc tcaatgccag ctcctgctcg ctgagttcac
      181 gaatcgcttt cgccgggctg ctgacaatca agtaattagt cggcatttct gctttggctt
      241 tcacaaatgc ggaaacacca acaatactgt tctcaccaat caccacaccg tccatcacta
      301 ccgcgttaat tcccactaat gtatggcggt ggttaatgca gccgtgaaga atagtcggag
      361 atacaggtat gtcagtacgt agtttgtacc gaatgtttgc cgataaaggt ttggtggtcg
      421 cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag
      481 atgatgaaaa actggcaggc attggctttc attggggatt ttccgaccag agtcattttt
      541 caacggtatt taaacaacgc tttgggatga cgccaggcga gaatcggcgt aaattccgct
      601 aactaattcg cgttgcatgt acagcaacgc gaattagtta aaacagatat ttgactatgc
      661 atttttcagg gcgaaaggca atttgacagg cgtttttgaa taatcatctt gtttccggaa
      721 aatagattaa tcgccttctc ctgtagcgtt atcccgatat tgcggcttta ctattaaaat
      781 tttcgcaccc atttaccgtt tatcccttgt acatattccc cgggtttggc ccgttctacg
      841 agcttttgcc cggcgagttt cgcgatatca tctaccgaca cattattttg ctttgccagt
      901 tgttgatagc tcgctttacg ttcggcatta atgtcggtta ctaatttctc agtttcagca
      961 tctgttttca gcgcaaccag ataaccataa aatgtttcgc ctacccgccc ctgagttctg
     1021 gcttcatcca gagtcaatgc cattacattg ctgcttacca agccaacaag aaatgcacaa
     1081 agaagtaatg ttcttttcat catcacctca aaaaagatcg ctacgggtct caagcagctc
     1141 ttcgacatct ttgtctgcct tgatgatgat ctcatgctca attttaacgt tcatattgat
     1201 agtgatcggt tccttaggtg cagcgacttc aatacgaggc gtacagccaa ccagcataaa
     1261 agatgacgtc aacgcagcca gtaaaatttt cattgtttat cctcacactc ttttccttgc
     1321 ggacagtcat ttcccggcag acgcgcgttc tgctccagcc atgcctggag attatcgcca
     1381 aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat gatggtaatt aagatttacc
     1441 gtaccgcttt taccatcaac gcgactggta cctgtaatat tggcctgcat ggttaataaa
     1501 ccgagattat ctaaattaat ttttgtcgat gaacggctaa tttccatata gcgcaaccag
     1561 ttaattgctg aacccgcggt catattgtct ttcaccaccg catctgcggt gtctttatcc
     1621 agccgcaagg tcattggtcc gctatttgcc agccagccgt ctttcactat ccatttttca
     1681 ttgtttaacc ataacggcag tgcaccacta aatgccccgg acatggcgaa ttgtttcgga
     1741 ttaacggcgc taaccagttc gctggatgag agattattca gtcgcaacag tgccgggtca
     1801 tgttgcggca tacgtaattg tttcatcagc acgttaccgc ctaacacatc gacactaaca
     1861 tcagtcaaca gcaatggttc ttcttcagtc cacggataac gcccttgcaa atcagccgta
     1921 atatttttcg ctgtcaccag attaatcact tcggcaattc gcaacgtaac ggggccgcga
     1981 gtccccagat gccaggctcc atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta
     2041 acctgattat ctggcatcca ggcactaccg cctttcaata cgccgtgccc ccccgcgcgg
     2101 aatccttgtt cgggcgctgc tgaaaatgcg acctgagcat acagttcacc atcgcgtaag
     2161 ttcatcttcc agtcgggtgg cgccaacggc tggaatacag tcagtgattg tttaggccac
     2221 caggcgttac cgcgcagacg aataccgtcc cagcgaccat ttacccgaac cgggccaatc
     2281 tcaccagcat gtaaatcgcc tttaaagagg aaatacgtcg gatcgcgccc atcgacacta
     2341 aattttaagg ttgatggcgg taacacactg ccgccagtga acagcgtttg cccggcgtcc
     2401 agtgacagag cgccgctgaa ggagggatgc tgtgcgtcac gtccccagac gacgggcttg
     2461 tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg
     2521 gtggagagat cggtcagcgt aatcgtgctg tcatgccact caccttttcc tgcgacatcc
     2581 caggtggcat tcatcggtgt aaaactccct tttccccagt agcgccactg ccagcggcca
     2641 gcgtcaggga gaaaatcatt cgccagccca tccatatgca gcacgaaatc gcccagttca
     2701 ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt
     2761 acacccgcta aaggccagcg gatttcatcg atgtccagcg aatcaatgac tcttcccttt
     2821 gaacgaagta acgcgccagg ctcaaaggtc agcgttgggt cagacagact tccacttagc
     2881 tgcgcaggta aacgggcata taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc
     2941 agaggcagct ggctgttatc catgcttaat tttcctgggc caaaatttaa caccgcgttg
     3001 cctttacccg cttgcccctg ggtcagcaca ctcagtcgcc cgctgaccag agcgttctcg
     3061 agccctgcct gccagttgtc gactttgaca cccagtcggc cactcaaagg aaaacctgca
     3121 tacggccagc tccagcgacc atcgcttacg gtcaattgtt gacgagtaat ttgccacggc
     3181 aaatcgagca acggatcgcc gttatcccgt gccagcacaa tcaattgccc gctattttcc
     3241 tgccagtcca gctcggcatc caccagtgaa ggctcctgcg gtaagtttaa cgtcgcagta
     3301 gcatgaccac ttacaggaag tccatccggc acgagcggca tagtaaattc ccccaccagt
     3361 tttaccggcg gctgattttc aaacgcgacg acatccagtt cgctgactgt aagttgttgc
     3421 cctttaagct ggccttgaaa tttaactttt tcgccctgat aacgcagttg ctggatatcg
     3481 gaggttaatg cgagagagag ttttccctgc cattcctgcc agggagaaaa aatcagttta
     3541 tcgatattga tccaggtgtt aggcaacatg gcctgccact gcgcgagggt tttgggagcg
     3601 gctggcgatt gctccgtctg cggcaatttc gccagacaag cagaatcaag ttctaccgtg
     3661 ccgacgttca ataaccagcg gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc
     3721 tgacaatctc ccaccagata acggagatcg gggatgatta aacctttacg cgtaatgcgt
     3781 gggctttcat ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc cagcgtggga
     3841 acccacagcc cgagcgtcat cagcagcgtc aacggcacaa gaataatcag taataacagc
     3901 gcgagaacgg ctttatattt acccagcatg ggtagttaat atcctgattt agcgaaaaat
     3961 taagcattca atacgggtat tgtggcatgt ttaaccgttc agttgaaggt tgcacctaca
     4021 ctaagcatag ttgttgatga a
//
LOCUS       DAPQZD010000270         3935 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPQZD010000270 DAPQZD010000000
VERSION     DAPQZD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.276"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(30..2510)
                     /locus_tag="RO454_004821"
     CDS             complement(30..2510)
                     /locus_tag="RO454_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDY0936519.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(2526..3200)
                     /locus_tag="RO454_004822"
     CDS             complement(2526..3200)
                     /locus_tag="RO454_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDY0936520.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3281..3823)
                     /locus_tag="RO454_004823"
     CDS             complement(3281..3823)
                     /locus_tag="RO454_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936521.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLVMRVAQD"
BASE COUNT         1098 a          956 c          728 g         1153 t
ORIGIN      
        1 atgcgaattt ccatagaacc acctgactat tactggcaaa tataattttt actttcatca
       61 atctctttac cgaaggtgat tgtgcatgaa agcccttgtt gcttatcaat tgccacatta
      121 accgatggcg gtacttcatt ggtgcgaata aataactggc ttccctggcc gacaacacca
      181 atattatgac catggatatc attgacttca taaccaaacg ttaatggttg cccatccgct
      241 cttaatgctt ttataaacca tggcttgcgc tgatcggtat caaaattaac cagtacaacc
      301 gcgccgcgat aaggggcggc aattttccgg ttgccacgta attctgcttc gctatcgctt
      361 tgtgacacat ccaacatcag gtaattttcc cgataaggtg tcattccgtc gtataccacc
      421 actccattac ggtttgttgt gcgatatttt tgcccattga cataagcatc tttaattcct
      481 ggcgcattca tcacagcaaa cgtttcagaa agacggtttg ccagattaac gccacccgac
      541 caggcgacaa tgccccctga aacactggct ccagcctgtc gataagcact cgactgacta
      601 taactgccat tcactgtcgc aaccggcgcg ttccaggtta aattcgcccc agctgtcgtt
      661 tcatttccct gatactgata actcaggttg accccatagt taaactgatc ccggcttcca
      721 acggttcctg ataatcccgt attatttgag gcaaccccct gatcatcaaa cgtcgttgag
      781 ttagacatat atatttgccg acgaggcgtc gtaacgtcat caccccaatc aaagggaatc
      841 gatataaaaa tattaaaacg tttctcttca tgatgattct cgtcataagc atggcttgcc
      901 gcgagggtat agcttatcct tcgcaggttg ttggaataac tcaactgata atccttactg
      961 ctgccgctac gcccccagta atctcgccat aacgtactta aggacacaga accccagcct
     1021 tctggcaatg actgactcat attggcagaa aagctatttt tacggccaaa atcgttctgg
     1081 taataatcgg caatgtcata gatatcgttt tcatcacggc gataattatc tttattgttt
     1141 gcccatacgt gatcattaaa tgtccgataa tcacgcgacg aataacgcca ggccgccaga
     1201 ccaaaacgtg tcgacgtttg gctcacaaat ttgttgtagg caatttgata actttgcccg
     1261 tcaaacacat cgccgttgtc ttgtttacta tgcgactttg tggcatcgac ggaaatggca
     1321 ccaaagcgtg tattccagcc agtccccaaa gtgaacgcgt aataattatt cgcgaccatc
     1381 gagccaccat acagcgttaa taagttatta aaaccatact gataacccgc ctggacaaaa
     1441 tcactttgtt tgctcgcccc ttcaatatgg ctacgacccg ccgcaaaatc atatttcgac
     1501 acgccgggtt gcagcatatt tggtaccgct gcataaggca ccagataggt ggttaccgaa
     1561 ccgtccgcct ctttcacgct aacatcaaga tccgctccac caccggccaa ctgcaaatct
     1621 gtaatcgcaa acgggcctgg aggaacctct ttctgataaa ccacaaaacc attctgttca
     1681 atagttacca gcgcgttact ctgagcaatc ccctgcactc gtggcgtaaa attttgtttc
     1741 gagttaggca acatctgcat atcacgaaac aaccgcacac cgctgaagcg aacagaatca
     1801 aaaatatcgc ttgatgtgta catatcaccc acacgaagcg tgccgagaag ttgggcaaat
     1861 ccacgttcca gatacagggt attgctcttc cacacccctg gattgttatt tgttttactg
     1921 aaactggcat cagaatgcag ttgccacccc aataaattta acccgctgtt aaaacgtaca
     1981 tatgtactct tgctattacc ggacgcttta taatcgctgt aatactgact catataataa
     2041 gaggtataaa acgcattaat accccgctcc cagttttcag gtggtacata gccaatttcc
     2101 agttcttcca cccaggcttg cgggacactg aaatcgagac gaaaaactcc gatatcccag
     2161 gtatagctcc cgccctgaat aagttgctta aatgttaaac attgcttacc gctggcaaag
     2221 ttatcggtat taatgcctaa ccgcttgatc atttcccttg ataaacatgt ttcttgcggg
     2281 ttgtctttaa caatgatctc atatttccca cgccattgct tattgacata aatatcgatg
     2341 tcatactgcc ccggcaaggg ttgattatca tcaagacgaa tatttgcaac ctgctggtct
     2401 ttcattccac ctatcatgaa atgggtatca aaggtttctt cagctgcata agcttcaatg
     2461 ccgatcaata acgctacgat tgctgatgca agtggggtca ttctcaacat aggcagctcc
     2521 tgcaattaaa ttttgtcact aatatagttg ccatggtcat cgataatggt cagatgccag
     2581 ttatttgcat tattactttt gacattaaca ctctgacttt ctaagggggc aatcataata
     2641 gtttcataat tgactttgac attattagct ttgacatccg aaatcgtcac ccaattagct
     2701 gagtcatttt ttatcaccaa tccattgcca ctgcgattta ccagtaactt tttaaaggtc
     2761 gctttattta ccggagcaat acccgctggc cggtaaaaca acttaattct gttttgcatc
     2821 gcaaacttca gtgcattctt accttcttgt tctggactat ttggtggaat atccagaaca
     2881 ttcagataaa aaatactctc tttattagtg ggtagtttat tcggcataat tttaattttt
     2941 acttgctgcc cggaatttgc ccctattttt gccactggtg gcgttaacat gaaaggaacc
     3001 tgaatttttt ctggtggtaa tgacgtatcg ccatcatcaa tccacgcctg catcagcgaa
     3061 gaacgatttc cctggttcat cagctgcacc atcacttctt tgtgctctgc cgggtaaata
     3121 atacgcgtcc cgtagataac aataccggca tgtgcaggaa ggaccataga agaaatgagt
     3181 aaagctaata atcctttcat cttaatgcct cttaaattaa aaggccgcca tggggatagc
     3241 ggccatgtta attacctgaa gaatcttgca attttattta ttaatcctgc gccacacgca
     3301 taaccaggga agataacaca tcacctgaat tcactgagtt atctgtcagt ttcaggtatt
     3361 ttgctttaaa cggaatggca tattgaccgt ccgctatttt ataactctcg atgttgaaag
     3421 gtgcagaaac atccaggaca tcgctgttat cacctaaata gagataataa cccacggtat
     3481 cgttgctact ggctatcgcc ttatcgtttc cggttatttt atcgttggta atcaacgaga
     3541 gttttacacc agcaacttct ttcggacagc taatttttaa catgctgctg gttacaccga
     3601 ctgcatcatc ataggtcgag tatttagctt taccttcaaa gacagtggtt ggtacagttg
     3661 gcatattaat acttactgaa tcgccacccg cttcgaattt acacggagat tctgcaatat
     3721 tccctttaat ggttaatgtt ccggtatcaa cgtctgcggc aaatacccca gcgctcataa
     3781 aaaaagaaga aaaaacagcg gcagcaataa ttgaacgttt cataatatgc tccttaataa
     3841 aatctatttt tggttgttaa gagtctgtat ctcttggaaa caaatttctc acaccaaaaa
     3901 agttgccgca atgtaaacac attgttttat tgatt
//
LOCUS       DAPQZD010000271         3919 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPQZD010000271 DAPQZD010000000
VERSION     DAPQZD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.277"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..432
                     /locus_tag="RO454_004824"
     CDS             1..432
                     /locus_tag="RO454_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936522.1"
                     /translation="MIADIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            550..1578
                     /locus_tag="RO454_004825"
     CDS             550..1578
                     /locus_tag="RO454_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDY0936523.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            1639..2583
                     /locus_tag="RO454_004826"
     CDS             1639..2583
                     /locus_tag="RO454_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDY0936524.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDNSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GVNDGSIVVNKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYVLNFRSFLEDLEVFVLPFERYC"
     gene            2583..2849
                     /locus_tag="RO454_004827"
     CDS             2583..2849
                     /locus_tag="RO454_004827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936525.1"
                     /translation="MLKSIINGATTTPAQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            3044..3919
                     /locus_tag="RO454_004828"
                     /pseudo
     CDS             3044..3919
                     /locus_tag="RO454_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
BASE COUNT         1142 a          938 c          950 g          889 t
ORIGIN      
        1 atgatcgccg atatttacga gaaaataatg tctgatctcg agtttgaccg agacaatctg
       61 gaagaagtct ggcgtagaca accccgcctg ttgatggaat atggctcaaa actcgcccat
      121 gcagaaagaa gtgtcgcaga agcaaaactt aaccttgaag ctgttgaagc aaagctatac
      181 gacacagagc gtaagaactt gagtatgaac ggcattaagt tcaacgaatc tgtactggac
      241 gctaaggtta aaacaaaccc acagtatctg tctaaacggc agaagttgga tgaagcacgg
      301 cacatcgcag acatatacaa acatgctgtc gccgcctttt cgcatcgccg agacatgatc
      361 gttcaggcgt caaagatggc catcgttgaa ttagagcgat taggctctga acgctttatt
      421 actccccgtt gatttttgat agataataag taagtactga tctatcattt aacagctcga
      481 aagagccacg aatgaacgaa agcccaacgc gcatagcgcc atcggccaaa tcacaacaag
      541 gagaaacaca tgtctaagac attacttgat ttgcttaaca aaactcgtga agacattgcc
      601 gccaaacgtg gtaacaacgt tgatctgact cgcttaaaag acggcgtcaa ctatatccgc
      661 atcttcccga ataaagacga cccaaacggt aagttcttcc agactttcgg tatgcactac
      721 gttaagtatc agaacgagga aggtaaagaa gcaaccaacg cttatatttg tgagcaacat
      781 actcacggtc gcgcttgtca gctatgcgaa atggtgatgg aaggtcgcgc tcgtcacaag
      841 ggtaacaaag caatggaaga acgcatcggt caaatgcgtg ccactcctcg ctacctggtc
      901 aacggcattc tttctgctcg tgaggatttc gcagatgctg agaaatgcca gttaatcgag
      961 ctgccgtcta ctgtattcga tgatatctgc aaagcaatca ccgaagacat cgctgatgat
     1021 atcggcaatc cactgagcaa agaggaaggc tacgcattcc tgattaaacg tactggctct
     1081 ggtcgcgata ccaaatatga cgtctcgcct aagcgtaaag tctacaaagg cgatatcgaa
     1141 gataaattct ggaacaccca gcatgatctg atcgcatacg caaatcaggc tgatgaaact
     1201 cgtcttctgt cgacagttcg cactatgggt cgtctgattg gcatcgctgc accaactgcc
     1261 gcagcatctg caccagcaat ttcctcaacc gcgaaaacat cggctgcggc actacctgga
     1321 tttggctctg tcactggtca tacagaagga gcgacggctg tagcaaccgc gcacacaccg
     1381 gcttctgaac caaccagtct ggttgatgaa gaaatcctcc gtgccgttga aactgaattt
     1441 aaaccagagg caagttccgc tgccgttgcc gtatcagtca aagagtctga agcagtcgca
     1501 gcgacatctg tagcaaccgc atctgcgacg gaagatgaag gtctggatga cctactgaga
     1561 gagctggact ctctgtaatc ccattacgtg accagtaagg cgtctacgga cgccttactt
     1621 tttggaagga atgtaccagt gaattatctc ttcgtagatg gcaatagcct gggttattac
     1681 caccaacaat ctgacaaatt gcacaacggc gaaatggaag tacaggctgc tttcggcttt
     1741 gttaagaacg tccgtcgtta tgcctccatc ctccatgccc gacctatgat tctttgggat
     1801 ggatttagtg acaagcgtcg cgacttttac ccggactaca aagcaaatcg cgacgacgat
     1861 cctgatatga aaaagatgaa ggaaggcttt gctatccaga agccatacat cctcaaaatg
     1921 atgaccgcgc ttggagttac ccaactcatt gcaaaagatg cagaagcgga tgatctggcc
     1981 gggctgctgg tatcccgcat ggcaccgcag ccaaccgttg aacacatcta tctgttaaca
     2041 ggcgatagcg actggcttca gttagttcgt gaaaacgtaa gctgggtaag cctgcgtgaa
     2101 gacgccaaaa acaagcaggt taattttgag caatttgcgg agctgacagg attcgccacg
     2161 cctcgcgcat ttttggaagc aaaagcatta caaggcgata actcggacaa cattagcggt
     2221 gttggtggca ttggtgctgg cggtgcgaaa gagctgctgc atgaatgggg aagtgtcgca
     2281 acgatggtac gcggcgtcaa cgacggctca atcgtggtta ataaagggcg tcataagacc
     2341 gccttcaaca aactagcgaa gaatgccttc aacgagaaaa caggctgtcg aatgctcgaa
     2401 gcgttcaaga gaaacatcac gctaatgaac ctgattgaga cgaagtttcc gcctaccgaa
     2461 atcgaaacaa tcaaaggcaa tcgtgacgtg aaagcattcg agcaactgtg ctacgtgctg
     2521 aatttccgtt cgttccttga agaccttgaa gtgtttgttc ttccattcga aaggtattgc
     2581 taatgcttaa atcgattatc aatggcgcta caaccacccc tgcccaactg gcaaaagaga
     2641 ttgtctttta tcacggtgag tacgctgtca tcgcactgcc gtcaattcta ggcgctgccg
     2701 gaatgaaagc gacagatcgc gagtttggat tagtcagcga gcaggtcgta aaaatcctcg
     2761 ctcgtgtatc cagactcctt aaccacgatg cgattgtatt cgatgaatcc gccgctttaa
     2821 aacgaatcaa cgaaacaaaa ggagcctgat catggcaaaa ggaaaatccg cactggcact
     2881 tgctctgaaa aagaaaatcg gtagcaacga cgaaattcag aaagtaactc attggattga
     2941 tacaggcttt ccaccgttaa acaaagctat ttctggtcgt tacgatggcg gcttcccatg
     3001 tggtcgtatc gtcgaagtat tcggtcctcc aagtgcaggg aaaacgttcc ttgcaacagc
     3061 agcgatgatc tccgctcaaa aacaagatgg tctggcggta ttcctcgatc atgaaaacag
     3121 ttttgacgtt ggcctggctg tcgccaatgg cctgaacgca gatgaggatg acggtcagtg
     3181 ggtatacaag cagccagata ccttcgaaga ctctgtagag ttgatcggca caatacttaa
     3241 attggttcgt gatgaagagc ttatccccga atcagcacca atctgtatcg tggctgactc
     3301 tcttgcgtct atggttccga actccaaagc cgagaagttc gaaaagatgg ctgaaggcac
     3361 tgccaaagac aaagatcagc taaacatgaa cgacaatacg gcactggctc gtgcgacgag
     3421 tgcgaacttc cctactctgg ctttgtgggc acgcaaatac aacgcctgca tcatcttctt
     3481 gaatcaggtt cgcacaaaaa tcggtgtaat gtttggcgac cctactacgt cgccaggtgg
     3541 agattcaccg aagttctacg cttctgtacg tatccgtctt ggtgcatcgg tgatgaagga
     3601 tggtaaagag aagatcggcc aggacgtagg cgcagaatgc atcaaaaaca aagttgcacc
     3661 accgtatggc aaatgcacct ggaaattcta cttcgatcct actcgtggcc tcgacgttat
     3721 cgaatcgctc gtcgagtaca tgctggaaga aggatacctg ccaaagaacg ccagcgggcg
     3781 agttgaaatt ggtgacaaga aatacaccaa atcgcagatc gtcgagatgt atcgggagaa
     3841 gccactggct gaaatcattg cggctttgca ggcaatcgac gaccgaagag caaaagacaa
     3901 ccccaccgag tcagtagaa
//
LOCUS       DAPQZD010000272         3916 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPQZD010000272 DAPQZD010000000
VERSION     DAPQZD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.278"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            27..521
                     /locus_tag="RO454_004829"
     CDS             27..521
                     /locus_tag="RO454_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936526.1"
                     /translation="MARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDELETTVRLLN
                     ERLDNQASVVANVGAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRSEPFDAGFIHHHTFVDDNYHHSSGASCHSGWDGGGCDTSSSSSYSG
                     SCCD"
     gene            531..719
                     /locus_tag="RO454_004830"
     CDS             531..719
                     /locus_tag="RO454_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936527.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            813..1238
                     /locus_tag="RO454_004831"
     CDS             813..1238
                     /locus_tag="RO454_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HDY0936528.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDA
                     KEIAILEKTIAALPAFSPFAGEIAQMSANIRARYEASPRSANAQAIRELSDIAGTPEA
                     VDVLCLCLDIADQDGIGEQEEQALKKIAQALQLSLDAYL"
     gene            1238..1396
                     /locus_tag="RO454_004832"
     CDS             1238..1396
                     /locus_tag="RO454_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDY0936529.1"
                     /translation="MLERFRLVIVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKAKC"
     gene            1536..2102
                     /locus_tag="RO454_004833"
     CDS             1536..2102
                     /locus_tag="RO454_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936530.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERLF
                     TILNFNSTFEEPTQIVALAHHFGVHYLAEKERADKLQATLDMVSETQGTDDGNKSETI
                     IASKDREIAGLKSSLTMLMSAINLMSSKAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELTHRHDLNKQPHKTQYAL"
     gene            2244..>3916
                     /locus_tag="RO454_004834"
     CDS             2244..>3916
                     /locus_tag="RO454_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDY0936531.1"
                     /translation="MNTALSIIDDASSNTAIDYRQEMNVIHEIVAECEKEIALMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDHSRLNRGWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEEKHWQFPYDIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKLGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTMAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVF"
BASE COUNT         1055 a          877 c          968 g         1016 t
ORIGIN      
        1 tctttctgac actggatgga gtccacatgg cacgtcaaac ctatttcact tctgtaacta
       61 aacgtcctcg ttctttacgt caaattttgg ccgaattatt tagcggtcgt gttatgtcac
      121 gccttgatga actagagact accgttcggt tgctgaatga acgtttagat aatcaagcgt
      181 cagttgttgc gaacgtgggg gcgattgttg cctctggttc ttcacgcgaa gcgaaaaatg
      241 cacggccttt agtgaaggag aaaaacaaca aggacagttc gaatggaaaa ttttcaaaga
      301 aagaggctga aaccaatggc ctacgttctc attatagttt cactggcgac ggtagccgtt
      361 ccagccggtc agagcctttt gatgccggat tcatccatca ccacaccttc gtcgacgaca
      421 attaccacca ctccagtgga gcgtcctgtc actctggatg ggatggcggt ggatgcgata
      481 cctcaagttc atccagttac tcaggatcat gctgtgacta aggcggttgt atgaactggt
      541 tttcaaatca ctttggaaaa atttggctgg caattcttgc cctcatggcc gccggttggg
      601 tatcgaacat tataaaactt gtttgctctg gcgatctcca gtttcaggct ggcatgacct
      661 tggctcgtgt agttgggatt tttatttttc cagtcggttc ggtacttggt tatttctgac
      721 ggttgttagt gcatatgcat tgaccgtctt tgcgtaagca atttatttaa ctagaaaaca
      781 acttgttttg acaaataaca aaaggaaaac acatgttagg tttcttcaaa aagaaaactc
      841 gtaaagcagt tatcgaagtc aaaaaaatgg agaaccgtga tgcggttgag gccaccgtgt
      901 ggggcgcgta catgatctcc tatgccgacg gcacatgtga cgcaaaagaa attgccattc
      961 ttgagaagac aattgcagct ctgcctgcgt tttctccgtt tgctggtgaa attgcccaaa
     1021 tgagcgccaa tatccgtgct cgttacgaag cgtcccctcg ttctgctaat gcacaggcta
     1081 ttcgtgagct gtctgatatt gcgggtactc cagaggcggt cgatgttctg tgtctgtgcc
     1141 ttgatattgc cgatcaggat ggcattggtg aacaagaaga gcaggcactg aaaaagatcg
     1201 cccaggcgct tcagttatca ctggatgctt atctctaatg cttgagagat tccggcttgt
     1261 gatcgtcatt gctcttctgg tgatggcggt gttggtggat tttacgggaa agatgatgtc
     1321 tgtcatttct gatggcgtcc tcattggttt ggcgatctac ttcgcttatc cgttagttcg
     1381 taaagcaaag tgttaatgac aagggccatt tggcccttgt gtttcgttga ccgaaataga
     1441 gagtttgcac ctttacgttt agcttgctcc ccttatatgc cacatcacaa taaagccaat
     1501 aagaaaacaa cttgtttaag cattaaggaa aacacatgtg cgagaaatgc aaagcaatag
     1561 ctgatgaaca aaacgcccta ttcgaagaaa tggatgctaa tgaacttgtc aaaatgttag
     1621 ccattcttcg aggaatagaa gacgtttcca tatttgagag actgtttaca atacttaatt
     1681 ttaattccac ctttgaagag ccaactcaga ttgtagcttt ggcacaccat ttcggtgttc
     1741 attaccttgc tgaaaaagag agagctgata agttgcaagc gactttggat atggtgagcg
     1801 agactcaagg aactgatgac ggcaacaaaa gtgagacgat tattgccagc aaagatcgtg
     1861 aaattgccgg gcttaaatcc tctctaacga tgttgatgtc tgcaatcaac cttatgtctt
     1921 ctaaagcagg ttataaaatg ccatcactaa acagcgacga tccgatggcc gttcgccagc
     1981 ttttaggtgc aatggccgac cagctcgacg acacaaagag tcgccttgaa gacatgatgc
     2041 gggagctcac gcatcgccat gacttgaata agcagccgca caaaacgcaa tacgcactct
     2101 aatacagcac cagtcgacag ggggcgacag cccccatttt tacgccttaa atctgctgta
     2161 acgcctatga atgcagcttt tatgcctttt caagtttgcg ataattacac caatcagaaa
     2221 acaaattatt taatggtgta attatgaata cagccctttc catcatcgac gatgccagct
     2281 caaacacggc tatcgactat cgtcaggaaa tgaatgtcat ccacgaaatc gtggccgagt
     2341 gcgagaaaga gatcgccttg atgtatcagg tacacgactt cgtttatggc gacgaacgcc
     2401 acaacatgat taatcgcctg ctgagactaa accatcgacc agatgaagat cactcgcgtt
     2461 taaatcgagg ttggttggat aaagtcgatc tggaatgggt gaaacagaat atttgggccg
     2521 agtactggag gaaggtcacg gacatgacta acgttttgct gatcatgcca gcttcccgtc
     2581 gagatgagtg gcgcgagcag tttatagagg gcaaacagga agtcatcaaa actgacagaa
     2641 ccggctacca gatgaaggtt aaagagttcg ttggtgtacc ggagttcaaa gcagaaacgg
     2701 tcatacccac gatgcttaat ttgctgaatg acaggcacaa atatctctct gagcgcgtgt
     2761 atggcttgtt taaggcgctg agtcctgctc acaagacaaa taagacaaac ggtttcagcg
     2821 agcggctgat aatcgctgac tgcatttctg atttctggcg ggacagcgtt agcgtgaact
     2881 atcgcaaaga ggactacatc gacgatctgc gtgtcttgct tcatttcttc gcgcacaaag
     2941 aatttattac catcaaccgc actgctgagg tgctatcagc tgcgtatagg gcaaacgact
     3001 gccagaccgg tgactggatg aacgtcgatg gaaatctgat gcgcgtgaag atgttcaaga
     3061 acggcaacgt tcactttgaa atacatcctg acgtggcctg gaagttgaat gaggtgctgg
     3121 cttacagtat gcctgctgca atccccgcgc catgccgaac tgccccaaaa acacgggcac
     3181 caaagcagtt cgggttaatc cagaagacga tctccgtgcc ggttcgcact gcgcttcgtg
     3241 acgggcgatt gagcaaagac aaaggcgtat ggtacttctc tgattcagct ctccagaagt
     3301 cgcaggtgga agagcttgag cgcacactga gcttcattgg cggcgtgcag gaggaaaagc
     3361 actggcagtt cccgtatgac atcggccata cgctaaatac gattgtggct accggtttaa
     3421 taccggatac aaaatcacac cagttctacc ctaccccacg cttgattgct gagtacgttg
     3481 ccagagccac tgaattgaag cttggtgaga agctgctgga gcctcaagcc ggacgtgggg
     3541 atcttctggc ctatattaac gccgatctgg aagatgttac ctgcatagaa atcgcacctc
     3601 tcttcgctga tatcctgcgt ggaaaagggt atacgaacac gatttgctgc gacttcataa
     3661 agtggtctga ggacaacgca ggttatcagt tcgacaaaat cgttatgaac ccgccgtact
     3721 cgcttggtcg tcatagagag cacacgatgg ctgcgctggg gcatctgaaa gtcggcgggc
     3781 gtcttgtagc agtattgccg ggcactgcgc caatactgga ctggatgacg atggataatt
     3841 acgtttatgc cagagggaag tcgtttacca acgagtttga agacacaggg atcacagtca
     3901 gcgtatacgt tttcaa
//
LOCUS       DAPQZD010000273         3890 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPQZD010000273 DAPQZD010000000
VERSION     DAPQZD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.279"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..922)
                     /locus_tag="RO454_004835"
     CDS             complement(<1..922)
                     /locus_tag="RO454_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0936532.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIE"
     gene            1079..1999
                     /locus_tag="RO454_004836"
     CDS             1079..1999
                     /locus_tag="RO454_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0936533.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            2172..3314
                     /locus_tag="RO454_004837"
     CDS             2172..3314
                     /locus_tag="RO454_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDY0936534.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            3787..3888
                     /locus_tag="RO454_004838"
     CDS             3787..3888
                     /locus_tag="RO454_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936535.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
BASE COUNT         1168 a          842 c          812 g         1068 t
ORIGIN      
        1 tttctatttt tactcttttc tccaccataa aatcaataaa agcacgaact ttagagggca
       61 ttctcacccg agatgaaaca tatatataaa atccaggaaa tgaaacggcc caatcctcaa
      121 gaatcggctg taacttgccc gcattgatat gttcagcgat tgcaaaatcc agatgttgaa
      181 tgatacccgt tccattcagc gccattcgta tcatgctttc gtcatcatta gaaatatact
      241 tacctgacac ctctatttcg aactcttcat taccccccac aacattcgaa aactcccatg
      301 ctgagagtgc accgctactt ttatgccgca accgaagaca attatggtta ctcaattcct
      361 gaggagtttc aggaataccg tgttctttta aataatctgg cgatgccaca gtaaccagtt
      421 taataagcgg agaaacagga atggcagtca tatgctcatc cagtccttgc tcaagacgta
      481 tgcccacatc acagccgtcc gcaataatgt tagataaacc gtcatccata atgagttcta
      541 tctttgtttt ggggtaacga gtaagaaact cgccaatatg aggctcaaca agcatccttg
      601 ctgcaacacg ggaggaattt attctcaaca gtcctgtagg ttcaagttgg gtatcaccga
      661 catctttcaa cgcatcatca atttgcccca aagccgacac cagcacttca tacagatgct
      721 gtccttcctc agtaagcgac atattgcggg ttgtacggta tatcagcttg gtgttaagac
      781 gttcctcaag agattttaaa ttctgggaca atgcggcccg cgtaataccc atttcagagg
      841 ctgctctgga aaaactgccg gcacgaacaa tcagcgcaaa ccaggcaagg gaaggcaaaa
      901 ttgaaggctc tattttcatc attgtaaacc ttaacttaac actgtgatag atattagacc
      961 attgatgata accaatacaa gtcatactat tacgccgttc gcgatatgaa cacgaatgcc
     1021 agttgcggat aatagaaatg atatttaaat ctgtttaggc aaatccctgg agcttgaaat
     1081 gaaaactgtt tcaataaaac acacttactg ggatatagct gctgatatct atttccctga
     1141 cgattttaat agtgaaaaaa agtatcctgc cattatcagt gcccatccaa ttggaagctg
     1201 caaagaacag acatcgggta gtgtgtatgg tgccgccctg gcaaaagctg gctttatcgt
     1261 cattgctttt aatgccagtt ttcagggaag cagcggtgga gaaccgcgtt atctggaaga
     1321 tccaacaatg cgagtaaaag acttcagcat tgtcgttgat taccttacca ccttacctta
     1381 cgttgatgcc gggcgtattg gtgtactggg tatttgtggt ggaggtggat atgccattaa
     1441 tgccgcaatg acagaacgtc ggattaaagc cattggtact gtcacaggtg caaactacgg
     1501 gcgtcttatg cgtgagggat ttacagcgtt taatcctatc ggagcactcg aagcaatggc
     1561 gcaacaaaga acagacgaag ctaacggcgc aaaacttcgc gtagatgatt tgcttccttc
     1621 ttctccggaa gcagctcttg aagcgggatt aacagaaata gacctttttg aagcaacaga
     1681 atattatcgt tcgccacgag gctgtgcgcc taatggggta aatcgttcgc tattttcaca
     1741 tcagactgtt gccgtgggct gggatgcttt tcatcttgca gaagtcctgt taactcaacc
     1801 actaatggtt gtagtaggcg atagagtggg cgcttttggt gcctatcgtg acggctgcga
     1861 aattattggt cgtgcagcct caaaacataa agagttggtc gtggttgaag gttattcaca
     1921 ttacgacctg tatgataagc cagaacctgt gaaacaggcg ctcgataaat taattccttt
     1981 ttacaaaaca catttgtaat aaccttgcag ggagattcat tatctccctg acatttatcc
     2041 tttattgact acacgacaat aataaaatta ctcaaaaaga attctattaa aggaaaaaag
     2101 catcaaaaag tagcaactca acttattaag attatacgca ttaaaatcag ataaacattt
     2161 gaggtattgt tatgacaaat aaacacccat cattgttttc tccttttatg ctgacagaaa
     2221 aaataaagct acgtaatcgt atcgttatgg ctcctatgac aacctggtca gctaatcctg
     2281 atggaaccat ctctgagcaa gaactcgaat tttataaacg tcgctcacaa aatgttggtt
     2341 tagtaataac aggatgtacc tatgtcacgc caagcggcat tgggtttact catgaatttg
     2401 ccgcgtatga cgaccgtttc attaacagcc ttgaaaagtt agcagctgca gccaaaagtg
     2461 gcggtgctcc ggctattttg cagattttcc acgccggtaa taaagccatc ccggagcttg
     2521 tgccaaatta cgatgttatt agtgccagcg catcaagcgt aaaatctggt gactttatga
     2581 agagagtggt acaaagcagg gaaatgactg aaaacgaaat tcaggaaacc attagagcat
     2641 tcggagatgt cacaaaaagg gcgattaaag caggttttga tggcgttgaa cttcatggtg
     2701 cgcacggatt ccttctgcag aatttcttct cccctctatt taaccagcgt aatgaccgat
     2761 ggggaggaga tcttgaagga cgtatgcgct tcccgctggc cgtattacag gaagtaaaaa
     2821 atgtagtcta tgaatatgcg acaaagccct ttgctatcgg ttacagaata tctcctgaag
     2881 aatctgtaac agggggactg cggatagagg atacttataa actccttgat cggctcattt
     2941 catccggtat ttcgtatata catacctcgc tggtcagtat taacgatagc taccctgttg
     3001 agtcgccaaa tggtccccga actattgaat tgattttaaa tcatattgca ggcagagtgc
     3061 cggtaatcgc cgcaggtaaa atacgcacac cgtctcaggc tcaagaagca atttccgctg
     3121 gcctgccact tgttgcgatc ggtaaagggc tggtgataaa tccagaatgg gtgactttag
     3181 ctgaatcagg tagaagtcat gaaattcaaa cagcccttaa tccacaacgc gttcctgaat
     3241 taaccattcc ggataaacta tgggatcaaa ttcaggcatc gaaaggaact ggctggtttc
     3301 cgttaatgga ttaaatatcg attactctcc cgagagtaaa actatcaaat caatgagtaa
     3361 ccgatacagc ctcgcaaatt ctttcagata attaacccgt cactgcggca cgttctctcg
     3421 caaggcgtgc ctgcagtgta atccagtatg tatccggcat agttgacggt ttaatagact
     3481 caattcctct ccctggttac ctacaacgat atcttgtata acaccccagg agatacgacg
     3541 ttacgatgag atgcgatatg ctcctcagcg ccaatcaacg ccaggactgg gccagtcttg
     3601 agatcagact cattcccgta actgatttca ggcgacatat atcaatgacg ttggggcaat
     3661 aaaagatagc cgatgtaata catctattca aatttgtgat acatgtcaaa aaattggttg
     3721 accaaactcg ttatttatat aagggcactt acgaagtgca ctctttttta aagcgaggaa
     3781 gtaccaatga aagagaataa agtacagcaa atcagtcata aactgattaa tatcgttgtt
     3841 tttgtcgcaa ttgtagaata cgcctattta tttctccatt tctattaata
//
LOCUS       DAPQZD010000274         3861 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPQZD010000274 DAPQZD010000000
VERSION     DAPQZD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.280"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            88..>3861
                     /locus_tag="RO454_004839"
     CDS             88..>3861
                     /locus_tag="RO454_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0936536.1"
                     /translation="MCSKRNIGDMKITVLNQRYKKHFGYPHILMGDVKVTVSAMIACA
                     VLLHPNKLFASVAGTSLPYQTYRDFAENKGEFRPGAENVPLYDKNGNPVTTLNKAPMI
                     DFSSNDSTGVATLVSPQYVVSVKHNVGYQYVKFGYADDSSYALVDRNNHWRDFHTPRL
                     NKIVTEVTPLDITNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGAYSYK
                     TGGIVNTPAISDWSFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLNSYAG
                     VNGNTNWYTIIPAGDVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGSTSWE
                     MHGNLGATWPASLNSGKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQTWKG
                     GGIIVNGEHLVDWQINGVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQRADD
                     NGLSQAFSSVSIVSGRPTLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLNGADD
                     GAILTNSGSMANVNLDFNSPDTTATIANIWHGHFTGNLNINNEVTAGTQNDFAIDGGV
                     NAQGSITQQNGRLFMQGHPVVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWENRQF
                     SMAELNLQNAEFNLARNASLNTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTLGKSK
                     ATAEDDQSRFNGHVQLQQGSALTINEHFAGGIDSADSATTITSTDTTLNQLSRFTQSS
                     LSLGEGAKLTATAGLLSDGTVSSNAGASLSLLSDQPGTMYSAQSWELSGQDTSLNVGA
                     GGIITGDINANDAASISFGTTDINQSTNYYGNINAPLASVTMKDTAWQVNKQSVAKSL
                     TLNGSTLSFNRFGQGGLTSDTLEATNSSFIINADGKAADTVTVNQALTGGNNTLVVIP
                     TTNSVKQGGDPVSLVTAPKNTQSNIFTLNPVSINAGFHSFTPQLDVLETDVNKQWRLE
                     GFYIQPDKAALRTGKSFMDLGYKNFITEINNLNDRMGDLRHTHGETGAWARLNSGSGS
                     ATDGFTGSYTHLQIGADRKHIIEGGELFTGVTATFTSSNNRGTGWSGRTKSTGIGVYA
                     SAMFDSGLYVDTIGKYVRHDNHYSSSALGMPEQDYGSHSWYLGAEAGWRFSLPDETYI
                     QPQTELIYGTVSENQFAWQFNGGEIYMQRKQMQPLIGRTGIEFGKTFRGKDWEMTALT
                     GINYQYDLFKPTVTAFKDLAGDTYINNGKDSRVVFNVGVNTKIKENTRISLNVE"
BASE COUNT         1163 a          758 c          934 g         1006 t
ORIGIN      
        1 agcaatgctt ggatttatta gttttaagat ctttcgagtc agaagatata tgaatagata
       61 gaatagaaat tcaattgtcg gaatgaaatg tgttcaaaaa gaaatatagg tgatatgaaa
      121 attacagtcc ttaatcagcg atataaaaaa cattttggtt atcctcatat tctgatgggt
      181 gatgtaaaag taactgtttc agcaatgatt gcttgcgctg ttttattgca cccaaataaa
      241 ttgtttgcct ccgtcgctgg aacctcactt ccctatcaaa cgtatcgtga ttttgctgag
      301 aataagggag agttccgtcc tggcgcagaa aatgttcctc tatatgataa aaatggcaat
      361 cccgtcacga cgctgaataa ggcacccatg attgacttca gctctaatga ctcgactggg
      421 gttgcaacac tggtttcgcc acagtatgtc gtcagcgtta aacacaatgt tggttatcaa
      481 tatgtgaaat ttggctatgc agatgattca tcctatgccc ttgttgacag aaataaccac
      541 tggagagatt tccacactcc ccgtctcaac aaaattgtca ccgaggttac acctctggat
      601 ataaccaatg cgggtacagc taagggaacc tatcaaaatg ctgaccgttt ccctgtgttc
      661 tatcgagtag gtacgggaac tcaatatgta aaagatacta acggaaaaat cacctattta
      721 atgggagcct actcgtataa aacaggtgga atagtaaata cgccggctat atctgactgg
      781 agttttgtca cgaataccac taatactcca ctctcaacct acggcactcc cggtgacagt
      841 ggctctcctc tttttgcctg ggatgcagac caaaataaat gggttttgtt agccgttctc
      901 aattcatatg caggggtcaa tggcaatact aactggtaca caataattcc tgctggtgat
      961 gtaaaaaata cgatgaaaca agatgccgat gcaccagtaa atacaaaaca aggtgaaggg
     1021 gatattcatt ggtcatatga cgaaaaaacg ggacttggtt cattaaccca gggaagcaca
     1081 tcatgggaaa tgcatggaaa tttgggggca acatggcctg cttcgcttaa tagtggtaaa
     1141 gatctaactt ttcagggcgg cggtactgta gtgttagaaa ataccgttaa tcagggggct
     1201 ggcacactta cttttgatga tgattatatt gtcaagcccg ttgatactca gacatggaaa
     1261 ggcggcggaa ttattgttaa tggtgaacat cttgttgact ggcagattaa tggggttact
     1321 ggtgatagtt tgcataaact gggtacgggt acgttaaaaa ttaacggtac cggtgtaaac
     1381 ccaggaagct taagtgtggg agacggaacc gttatattgg cacagcgtgc tgatgataac
     1441 ggactgagcc aggcgttcag ttctgtcagt attgtaagtg ggcgtcctac tctggtgcta
     1501 aacgacgaca aacaaattaa tccggataat atcaaatggg gctatcatgg cggcaagctc
     1561 gacattaatg gaaacagttt gacttttcat aaacttaacg gggctgatga tggagctata
     1621 ctgactaaca gtggctccat ggctaatgtt aatctggatt tcaacagccc agatacaaca
     1681 gccacgattg ctaatatctg gcacggccat ttcaccggta atctaaatat caataatgag
     1741 gttaccgcag gaacacagaa tgatttcgct attgatggtg gagtgaatgc tcaggggagt
     1801 ataacccagc aaaatggtcg gctatttatg caaggacatc cggttgttca cgccgtcagc
     1861 agtcaggacg tagcaaataa gctaaaggcg ctgggtgaca attcagtact gacccaacca
     1921 gtgtcattta ctcagaacga ttgggaaaat cgacagttca gtatggcgga gcttaactta
     1981 caaaatgctg aatttaacct tgcaagaaac gccagcctta ataccagaat taatgccgag
     2041 cattctaccg tgacgttagg tagtgaggat ctttacattg atctcaatga cgggaatggc
     2101 gtggcaacca aaccaacatt gggtaagtca aaggcaaccg ctgaagatga tcagagtcgt
     2161 ttcaatggac atgtacagtt gcaacaagga tcggcactaa ctattaatga acattttgcc
     2221 ggcggtattg atagtgcaga tagtgcgacg acgataacct ctacagatac cacgcttaat
     2281 cagctcagcc gtttcaccca atcttctttg tcattaggtg aaggggcaaa attgaccgct
     2341 acagccggat tactttctga cgggacggta agcagtaatg ccggggcgag cctttccttg
     2401 ctatcagacc agcctggaac gatgtattcc gcccaaagct gggagcttag cgggcaagac
     2461 acgagcttaa atgttggcgc gggtgggatc atcactggag atatcaatgc caatgatgcg
     2521 gccagcatta gctttggcac gactgatatt aaccaatcca caaattacta tggaaatatc
     2581 aacgcgccgt tggcaagtgt aacaatgaag gacactgcct ggcaggtaaa caagcagtcg
     2641 gtggctaagt cgctcacttt gaatggttca acactgagct ttaatcgttt cgggcaaggg
     2701 ggactgacgt cagatacgct ggaggccact aacagttcgt tcattatcaa tgccgatggt
     2761 aaagcagcag atacggtgac ggtaaaccag gcattgacag gggggaataa cacactggtt
     2821 gttattccta cgacaaattc agtaaaacag ggcggtgacc ctgtgtcatt ggttactgcc
     2881 ccgaaaaata cacaaagcaa tatatttaca cttaacccgg tttcaataaa tgcagggttc
     2941 catagtttta caccgcaact tgatgtgttg gagaccgacg ttaataagca atggcgtctg
     3001 gagggatttt acatacagcc tgataaagcc gctttgcgga caggcaagag ttttatggat
     3061 ctcggttata aaaactttat tacagaaatt aataatctga atgatcgtat gggggactta
     3121 cgccataccc atggtgaaac tggggcatgg gctagactta acagtggttc gggctcagct
     3181 actgatggat ttaccggaag ttatacgcac ttgcaaattg gggcagacag gaaacatata
     3241 attgaaggcg gagagttatt tactggagta acggcgacat ttacaagcag taataaccgg
     3301 ggaactggtt ggtcgggaag aaccaaatca acgggtattg gtgtgtacgc ttcagctatg
     3361 tttgactctg gactctatgt agatactatc gggaaatatg tgcgtcatga caatcattac
     3421 tcatcaagtg cgttaggaat gccagaacaa gattatggct cgcactcctg gtatttgggc
     3481 gctgaggccg gttggcgttt ttccttacct gacgaaacat atatacagcc gcaaacagag
     3541 ttgatctacg gtactgtatc tgagaatcag tttgcctggc aattcaatgg gggtgagatc
     3601 tatatgcaac gtaagcagat gcaaccattg attggtcgca caggaattga atttggcaaa
     3661 acattccgcg gcaaagactg ggaaatgaca gcgctgactg ggattaatta tcagtacgat
     3721 ttatttaagc ctactgtgac agcgttcaag gatctggcag gtgatacata tattaataat
     3781 ggtaaagata gcagagtggt attcaatgtc ggggtaaata ctaaaattaa agaaaacact
     3841 aggataagtc tgaatgtgga g
//
LOCUS       DAPQZD010000275         3861 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPQZD010000275 DAPQZD010000000
VERSION     DAPQZD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.281"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(82..426)
                     /locus_tag="RO454_004840"
     CDS             complement(82..426)
                     /locus_tag="RO454_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDY0936537.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            558..2468
                     /locus_tag="RO454_004841"
     CDS             558..2468
                     /locus_tag="RO454_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDY0936538.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2526..3221
                     /gene="tsaB"
                     /locus_tag="RO454_004842"
     CDS             2526..3221
                     /gene="tsaB"
                     /locus_tag="RO454_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDY0936539.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3261..3842
                     /gene="yeaY"
                     /locus_tag="RO454_004843"
     CDS             3261..3842
                     /gene="yeaY"
                     /locus_tag="RO454_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDY0936540.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
BASE COUNT          896 a         1004 c         1077 g          884 t
ORIGIN      
        1 tcttaatctc tggtcgattt ttgcaaaacc gaccagtatt gtcgataaat tatcgtctga
       61 cttattttgc ttcgataaag cttacaccgc agccacaatc ttaatttcaa ctttatactt
      121 cgggttcatc aaacctgctt gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca
      181 ggcatcccac gctttattca tcgccgcgaa gtcgttttta tcggccagga aaatggtggc
      241 atccagaatg ctcgatttat tgctgccctg tttttccagc acggcgtcaa tctgcgccag
      301 cgtgttggcg gtttgctcaa aggcatcggc gtcgaggttt tccggtacac cagtgtagta
      361 gagcgtgttg ttgtggatta ccacatcaga ccagcgggct tcggcatcga tacgaacgat
      421 agtcatcatt ttgtcctcat tatacttcca ttttcgtgct gcaaagactg ccacaatgtg
      481 aggtcgccgt caccacttca actggcgaaa gcgccctcat cctgacataa tccctgttca
      541 aatcaacagg gggtagtgtg acggacgatt ttgcacaaga cggtcagctg gcgaaagcga
      601 taccaggctt taagccgcga gaaccacagc gacagatggc ggtagccgtc acccaggcga
      661 tagaaaaagg ccagccgctg gtggtggaag ccggaaccgg tacgggcaaa acctacgctt
      721 acctggcccc tgcgctgcgg gcgaaaaaga aagtcataat ctcgaccggc tcaaaagcgt
      781 tgcaggatca gctctacagc cgcgatttgc caacggtctc aaaggcattg aaatacacgg
      841 gcaatgtggc gctgctgaaa gggcgctcaa actacctctg cctcgaacgt ctcgaacagc
      901 aggctttggc ggggggcgat ctgccggtac aaatcttaag cgatgtgatc ctgctgcgct
      961 cctggtctaa tcaaacaatc gatggtgata tcagcacctg cgtcagcgtg gcggaagatt
     1021 cacaggcgtg gccgctggtc accagcacca acgataactg cctcggcagc gactgcccga
     1081 tgtataaaga ttgctttgtg gtcaaagcac gcaaaaaagc gatggacgcc gatgtggtgg
     1141 tggtaaacca tcatctcttt ctggcggata tggtggtgaa agagagcggt tttggcgaac
     1201 tgatcccgga ggcggacgtc atgatcttcg acgaagccca ccagctgccc gacattgcca
     1261 gccagtattt tggtcagtca ctctccagtc gacaactgct cgacctggca aaagacatca
     1321 ccatcgccta ccgcaccgaa ttaaaagaca cccagcagtt acaaaagtgc gcggatcgtc
     1381 ttgcccagag tgcgcaggat tttcgcctgc aactcggtga gcctggttat cgcggtaacc
     1441 tgcgtgagct gttagctaat ccgcaaattc agcgggcatt tttactgctc gatgacactc
     1501 tggaactttg ttatgacgtg gcgaaactgt cgctggggcg ttccgccttg ctggatgcgg
     1561 catttgagcg cgccacgtta tatcgcacac ggctgaaacg gctaaaagag atcaatcagc
     1621 cgggctacag ctactggtac gaatgcactt cgcgccattt tactctggct ctcacgccgc
     1681 tcagcgtggc ggataaattc aaagagttaa tggcgcaaaa aaccggtagc tggatcttta
     1741 cctcagcaac gctgtcggtg aacgacgatc tgcatcattt cacctcgcgg cttggcattg
     1801 aacaggcaga gtcattgctg ttacccagcc cctttgatta cagccgccag gcgttactct
     1861 gtgtgccgcg caacctgccg caaaccaatc aaccgggttc cgcacggcaa ctggcggcaa
     1921 tgctgcgacc gatcatcgaa gctaacaacg gtcgttgttt tatgctttgt acctcgcacg
     1981 ccatgatgcg cgatctggcc gagcagttcc gcgctaccat gacgcttccc gttttgttgc
     2041 agggggaaac cagcaaaggg caactgttgc agcaatttgt cagcgccggt aacgcgcttc
     2101 ttgtggcaac cagcagtttc tgggaagggg tggacgtgcg tggcgataca ttgtcattgg
     2161 tgattatcga caaattgccg tttacctcgc cggatgatcc actattaaaa gcacgcatgg
     2221 aagattgccg tttgcgcggt ggtgatccgt tcgatgaagt acaactgccg gatgcggtca
     2281 ttactctcaa gcagggggta gggcgattga ttcgcgacgc cgacgatcgc ggcgtgctgg
     2341 tgatttgcga caatcggctg gtgatgcgcc cttacggcgc gacgtttctc gccagtctgc
     2401 cgccagcgcc gcgcacccgt gacattgccc gtgcggttcg tttccttgcg ataccatcct
     2461 ccaggtaatt tgttacggag tatggtatga tgcgcgcctt tttattgatc taagcacgaa
     2521 gacccatgcg aattctggct atcgataccg cgacagaggc ctgctctgtc gccctgtgga
     2581 acgacggtac tgtcaacgct cattttgagc tttgccctcg tgaacatact caacgaatct
     2641 taccgatggt gcaggatatc ctgaccacca gcggaacttc cctgactgac ataaacgctc
     2701 tggcttacgg gcgcggcccc ggtagcttta ccggcgtgcg cattggtatt ggtattgcgc
     2761 aagggctggc acttggcgca gagctgccga tgattggcgt ctccacgcta atgacgatgg
     2821 cgcaaggggc gtggcgcaaa aacggcgcaa cccgcgtgct ggcagctatt gacgcgcgaa
     2881 tgggcgaagt ttactgggcc gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag
     2941 aaaccgaagc cgtactcaaa cccgaactgg ttcatgaacg aatgcaacag ctttccggcg
     3001 aatgggtgac ggtagggacg ggctggcaag cctggccgga tctcggtaaa gagagcgggc
     3061 tggttttgca cgatggcgaa gtgttactgc ccgctgcaga agatatgctg ccgattgcgt
     3121 gccagatgtt tgccgagggt aaaacggtgg cggtggaaca tgccgaacca gtttatttac
     3181 gtaacaatgt cgcatggaag aaacttccgg gcaaagaatg aatcttaaga gtatgccctg
     3241 agaaaaaaag gagtcgcatc atggcggttc aaaagaatgt tatcaaaggc atactggcag
     3301 gtacgtttgc gctaatgctg agcggttgtg ttactgtgcc ggacgccatt aaaggcagta
     3361 gtcccacgcc gcaacaagat ttagttcggg tgatgagtgc gccgcagctg tacgttggtc
     3421 aggaggctcg ctttggcggc aaagtggttg ctgtacaaaa ccagcaaggg aaaacccgcc
     3481 tggaaattgc caccgtacca ctggacagcg gagccagacc gacgctggga gaaccttctc
     3541 gcggtcgcat ttatgccgat gtgaacggtt ttctggaccc ggtggatttc cgcggacaac
     3601 tggtgacggt agtcgggcca atcaccggtg cggttgacgg caaaatcggt aatacgccct
     3661 ataaatttat ggtgatgcaa gtaacgggtt acaaacgttg gcatttaacc cagcaggtga
     3721 ttatgccgcc tcagccgatt gaaccatggt tttatggcgg tcgtggctgg ccctatggct
     3781 acggcggatg gggctggtat aatcccggcc ccgcgagagt acaaacagtt gtaactgaat
     3841 aattgcttgt ttttaaagaa a
//
LOCUS       DAPQZD010000276         3841 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPQZD010000276 DAPQZD010000000
VERSION     DAPQZD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.282"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..701
                     /locus_tag="RO454_004844"
     CDS             <1..701
                     /locus_tag="RO454_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDY0936541.1"
                     /translation="VTITEAVEITTKAIKQKITPSDIYRHALSGNILLSIYFQSPVIL
                     KKIQTFNGKIKFRQFVGDLLDKLCMLDSDGFIYGQNLRLCTEARYICPVQQIIDTPLL
                     RKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAE
                     DMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRF
                     DATNSELCAERHEC"
     gene            628..2750
                     /locus_tag="RO454_004845"
                     /pseudo
     CDS             628..2750
                     /locus_tag="RO454_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2920..>3841)
                     /locus_tag="RO454_004846"
     CDS             complement(2920..>3841)
                     /locus_tag="RO454_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDY0936542.1"
                     /translation="FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTM
                     QDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMK
                     CYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRS
                     LGSYIALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYN
                     NVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMAD
                     VSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1123 a          884 c          868 g          966 t
ORIGIN      
        1 gggtcactat aacggaagca gttgagatca ctaccaaagc cataaaacaa aaaattactc
       61 ctagcgatat ttatcgtcat gccttgagtg gcaacatcct actatcgatc tatttccagt
      121 ctcctgtgat acttaagaag atacaaactt ttaatggaaa aataaaattc aggcagtttg
      181 tgggagacct acttgataaa ctatgcatgc ttgacagtga cggttttatt tatggtcaaa
      241 atttaagact ctgtacagaa gccagatata tttgtcctgt gcaacaaatt atagataccc
      301 ccctgttacg caaactcaac cagtttcgta cgtttgttcg taatgtccgc ccgggtgatg
      361 aactggatgt ccaggcacaa gttagtgaaa aaaatttaac cccgccgccg ggtaacagca
      421 gtggcaacct tgagcaacag atagccagta cttcacagct aattggatct ctgctcgccg
      481 aagatatgaa cagcgagcaa gcggcaaaca tagcacgtgg atgggcctct tctcaggctt
      541 caggggtaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg ctgggcgtgg
      601 atgaagattt cagcctgaag aactcccgtg acggcaaccc tcgaaaatgg cgattcgatg
      661 caacaaacag tgaactatgt gccgaacgtc acgaatgctg aaatcacgct ggcagcctcg
      721 aaggatccgg tgattgccga caataacgat ctcacgacac taacagcacc gtcgctgata
      781 cagagggcaa tgcgatagcc aacactgagg taacatttac tctaccggaa gatgtgaagg
      841 cgaacttcac gctgagcgat ggcgacaaag cggttactga tgccgatggc aaagcgaaag
      901 tcacgctgaa aggtacaaaa gcaggcgctc ataccgtgac agcatcgatg gttggcggta
      961 agagtgagca gttggtggtg aactttactg cggatacgct cactgcgcag gttaatctta
     1021 acgttaccga ggacaatttc atcgccaata acattggaat gaccaggcta caagcaacag
     1081 tgactgatgg aaacggcaac ccagtagaag gtataaaagt taatttccgt ggaacctccg
     1141 tcacgctaag cagcaccagc gttgaaacgg atgatcaggt ttttgctgaa attcttgtga
     1201 caagcaccga ggtcggacta aaaacagttt cagcctctct ggcagataaa cctactgaag
     1261 tcatctcgcg attactgaat gccaaggtag atgttaattc tgcgacgatt accagtcagg
     1321 agatacctga aggtcaggtc atggtcgcac aagacatagc agttaaagct cacgttaacg
     1381 accagtttgg caacccggtg acgcatcaac ccgcgacatt cagtgcagcg ccatcctcgc
     1441 aaatgatcat cagccagaac acggtctcta ctaatacgca gggagtagcc gaggtcacca
     1501 tgacgtctga aagaaacggc tcgtatactg taaaagcatc cctagcgaat ggagcttcac
     1561 ttgagaaaca actggaggct attgatgaaa aactgacact cacgtccagt ccgcttatcg
     1621 gtgtcaatgc tcctaaaggc gctactctga cggcaacgct aacctctgca aatggcactc
     1681 cagtggaggg tcaggtcatc aactttagcg taacgctaga aggtgcgacg ttaagtggcg
     1741 gaaaagtgag aaccaactct tcagatcagg ctccagtcgt tctgaccagc aataaagtcg
     1801 gtacatatac ggtgactgca tcgttccata acggcgtcac aatacagaca cagacaaccg
     1861 tgaaggttac tggcaaccca agcaccgcac atgttgctag ctttatcgct gatccgtcga
     1921 ctatcgccgc caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg
     1981 gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctcc accacattaa
     2041 cgtcattaac agccgtgacc gatcaaaacg gaatcgcgac aacaaacgtg agatgagcga
     2101 taacagggag cgtcactgta agcgcagtca taagcgcagg tggaatgcaa acagtagata
     2161 tatcgctagt ggcaggccca gcagacgcct cgcagtccat ccttaagaac aaccagtcat
     2221 cactgaaagg ggactttacc gatagtgctg agctacatct tgttctgcac gatatatcag
     2281 gcaatccgat caaagtttct taagggatag aatttgtgca atcaggtaca aacgtgccct
     2341 atatgaaaat tagcgcaatt gattacagtc aaaatatcaa cggtgattac aaagctactg
     2401 ttacaggagg cggagagggt atcgcaacgc tgatccccgt attgaatggt gttcatcagg
     2461 cgggtctgag taccacgata gaattcatta gcgcggaaac cagaccaatg acgggtacag
     2521 tgtcagtgaa tagtgcaaac ctaccgacag cttcattccc ttcgcaggga tttaccgggg
     2581 cgtattatca gttgaataat gacaactttg caccaggaaa aacggcggct gattattcgt
     2641 tttcaagctc ggcctcctgg gtaggcgttg atgctactgg taaagtgaca ttcaaaaatg
     2701 acggtgatag caatactgta ataatcacag cgcccccaag atccggtggt gctatttacc
     2761 aaacagttcc tcctgaatcg cgttcggtgt gactatgatc attttcggtg agtattgctt
     2821 tgatccaaag gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc
     2881 gttcataaca gatcggcatt actatgcata agtactgcct caaattctcg ccaaaacact
     2941 ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac
     3001 tgactccgaa acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac
     3061 aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcaat
     3121 ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta
     3181 gacattatta taacaatcca ctaatgccct ggctttatct tcccctttgg gtccataaac
     3241 gatcactatt ggaatatctg tttcactttg aatttttgct attagatttt ctgcaatcga
     3301 taatgaaaat gtacgttcct gcgagctacc ttctaaattg agcgcaatgt aagatcctaa
     3361 cgatcgcatt tcctcgcgta cctcatcgag tacatcatcg cttaatggca attcatatat
     3421 tgacctgact gccggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacagg
     3481 agcccggaga ttctgatcca tccgggacgc gttcttacac aaaggggaat agcacttcat
     3541 ggttaaaccg acgacctgga aattcgtttt tgctttcaac tgactgataa atatcatcgt
     3601 tttcaggttc tttttacgca tcgcctctat acaaagatcc ggcgtaccat attgcagtgt
     3661 tatgttcttc gctaaatctt ttatttcttt taatgttgcg tgatcctgca tagtcattgt
     3721 gactaatgtt aatttagtct gttcaagttt gagcgcatta aagacttcta aattaattgt
     3781 cgacgtaaca attaatagat gcttaatttt atgcaattca agcgcccgaa taacaggaaa
     3841 g
//
LOCUS       DAPQZD010000277         3841 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPQZD010000277 DAPQZD010000000
VERSION     DAPQZD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.283"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            63..1682
                     /locus_tag="RO454_004847"
     CDS             63..1682
                     /locus_tag="RO454_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDY0936543.1"
                     /translation="MAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTM
                     DDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFT
                     NGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAAV
                     GKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSP
                     SLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPEQY
                     QKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLP
                     LNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKD
                     LKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPH
                     NPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGV
                     VREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1734..2891
                     /locus_tag="RO454_004848"
     CDS             1734..2891
                     /locus_tag="RO454_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDY0936544.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            2903..3814
                     /locus_tag="RO454_004849"
                     /pseudo
     CDS             2903..3814
                     /locus_tag="RO454_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
BASE COUNT         1169 a          849 c          806 g         1017 t
ORIGIN      
        1 tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg
       61 gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa
      121 acgttgcgtt ctcagacttt acgccgactg aatacagtac caaaggaaag ccaaatatta
      181 tcgtactgac catggatgat cttggttatg gacaacttcc ttttgataag ggatcttttg
      241 acccaaaaac aatggaaaat cgtgaagttg tcgataccta caaaataggg atagataaag
      301 ccattgaagc tgcgcaaaaa tcaacgccga cgctcctttc attaatggat gaaggcgtac
      361 gttttactaa cggctatgtg gcgcacggtg tttccggccc ctcccgcgcc gcaataatga
      421 ccggtcgagc gcccgcccgc tttggtgtct attccaatac tgatgctcag gatggtattc
      481 cgctaacaga aactttcttg cctgaattat tccagaatca tggttattac actgcagcag
      541 taggtaaatg gcacttatca aaaatcagta atgtgccggt gccggaagat aaacaaacgc
      601 gtgactatca tgacaacttc actacctttt ctgcggaaga atggcaaccg caaaaccgtg
      661 gctttgatta ctttatggga ttccatgctg caggaacggc atattacaac tccccttcac
      721 tgttcaaaaa ccgtgaacgc gtccccgcaa aaggttatat cagcgatcag ttaaccgatg
      781 aagcaattgg cgttgttgat cgtgccaaaa cacttgacca gccttttatg ctttacctgg
      841 cttataatgc tccgcacctg ccaaatgata atcctgctcc ggagcaatat cagaagcaat
      901 ttaataccgg tagtcaaaca gcagataatt actacgcttc cgtttattct gttgatcagg
      961 gtgtaaaacg cattctcgaa caactgaaga aaaacggaca gtatgacaat acaattattc
     1021 tctttacctc cgataatggt gcagttatcg atggtcctct gccgctgaac ggggcgcaaa
     1081 aaggctataa gagtcagacc tatcctggcg gtactcacac cccaatgttt atgtggtgga
     1141 aaggaaaact tcaacccggt aattatgaca agctgatttc cgcaatggat ttctacccga
     1201 cagctcttga tgcagccgat atcagcattc caaaagacct taagctggat ggcgtttcct
     1261 tgctgccctg gttgcaaaat aagaaacaag gcgagccaca taaaaatctg acctggataa
     1321 catcttattc tcactggttt gatgaggaaa atattccatt ctgggataat taccacaaat
     1381 ttgtccgcca tcagtcagac gattacccgc ataaccccaa caccgaggac ttaagccaat
     1441 tctcttatac ggtgagaaat aacgattatt cgcttgtcta tacagtagaa aataatcagt
     1501 taggtctata caaactgacg gatctacagc aaaaagataa ccttgccgcc gccaatccgc
     1561 aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat cgataacagc cagccaccgc
     1621 ttagcgaggt aaatcaggag aagtttaaca atatcaagaa agcactaagc gaagcgaaat
     1681 aactaaacct ttatgcggcg gatttttccg ccgccttatt gagcgagata gcgatgcacg
     1741 ttacagccaa gccctccagt tttcaatgta atctcaaatg tgattactgt ttttaccttg
     1801 aaaaagagtc gcagtttact catgaaaaat ggatggatga cagcactctg aaagagttca
     1861 tcaaacaata tatcgcagcg tctggcaatc aggtctattt tacctggcaa ggcggtgaac
     1921 ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa cgctatgcag
     1981 gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat aatgaatggt
     2041 gtgcctttct caaagaacat gagtttctgg ttggtatctc gatcgatggc ccccaagaac
     2101 tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa gtgatagcag
     2161 ccatcgagcg tctgaaatca tatcaaatag agtttaatac gttaaccgtt attaataaca
     2221 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
     2281 tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
     2341 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
     2401 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatc cgtcagtttg
     2461 aaagctttgt cagccgtttt ttggggaatg ggcataccag ttgtattttc caggagtcct
     2521 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
     2581 atccacagta caaaattgga aacattaata aatcggaact caaaacgatg aacagtgtac
     2641 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgta tataaaccta
     2701 tctgcaatgg tggttgccct aagcatcgta ttactaaagt aaacaatgag actgtttcct
     2761 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     2821 tggagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     2881 agaataatta ataacgctcc cccacagcac gattaacgac aatatttggg catcgctaca
     2941 aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca
     3001 gcagatgtat gagacgcgct atgctgataa ccgcacgaaa ttaccgcaag caaatcagat
     3061 tgcacagttt actgccgcag atgcactggc tgccgatcgc cagttgtttt catctccagc
     3121 ggatatcacg tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg cgttaattac
     3181 gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac
     3241 tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaaccag tggcacaggt
     3301 ttcacagtgg aagcgttata attcccggac tcctgttaat ctggcgacgc gtatggcgct
     3361 cgatgctttt aacgtcgcac tggcaaaaga tctacgcatt aatattcgtg aacaggcatc
     3421 cggggcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca
     3481 tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga
     3541 agtgatggtt aagcgtctgg ccaaagggat cagtgagcaa gaactgaacg aataccagca
     3601 aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacaccat
     3661 tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt
     3721 gaaacaaatg acggtagaga atgttaacac tgctgttaaa caatatcttt ctcatccggt
     3781 gaatacctat accggagtat tattgccaaa ataatcacag ccccgtcaac acatcgttgg
     3841 c
//
LOCUS       DAPQZD010000278         3815 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPQZD010000278 DAPQZD010000000
VERSION     DAPQZD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.284"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            14..198
                     /locus_tag="RO454_004850"
                     /pseudo
     CDS             14..198
                     /locus_tag="RO454_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            159..278
                     /locus_tag="RO454_004851"
     CDS             159..278
                     /locus_tag="RO454_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDY0936545.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            313..655
                     /locus_tag="RO454_004852"
                     /pseudo
     CDS             313..655
                     /locus_tag="RO454_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1022..1288)
                     /gene="minE"
                     /locus_tag="RO454_004853"
     CDS             complement(1022..1288)
                     /gene="minE"
                     /locus_tag="RO454_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDY0936546.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1292..2104)
                     /gene="minD"
                     /locus_tag="RO454_004854"
     CDS             complement(1292..2104)
                     /gene="minD"
                     /locus_tag="RO454_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDY0936547.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2128..2823)
                     /gene="minC"
                     /locus_tag="RO454_004855"
     CDS             complement(2128..2823)
                     /gene="minC"
                     /locus_tag="RO454_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDY0936548.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3343..3711
                     /locus_tag="RO454_004856"
     CDS             3343..3711
                     /locus_tag="RO454_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936549.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
BASE COUNT          969 a          856 c          910 g         1080 t
ORIGIN      
        1 gtgtgattgt tatgtgagca gaagatattc attagcaacg attacattag tcattttatt
       61 ttgtggacgg cctcattgtc gaaaaataag cgtacgacag tattatcaga aaagagtgtt
      121 ttttatccaa caacacttca gcgcactgcg tttaaaaaat gcctctttct tatacgggat
      181 atcatcattt catcatgatg tctttgatga gcggtgaaca caatacattt gcgctgtctc
      241 ttcaggatga atcccctggt ctggtgcctg cgggctgatg ttgcagcaga gctaaggtca
      301 cttagacgct actatcattt atccaatgca tggaatcgaa atcagtcgat acccgcagta
      361 tatatcgtga actgggcgca acgctgagct acaacatgcg tctggggaac ggtatggaaa
      421 ttgaaccctg gctgaaggcg gctgtgcgca aagaatttgt cgatgataac cgggtgaagg
      481 tgaatagtga cggtaatttt gtcaatgatt tgtcgggcag acgtggaata taccaggcag
      541 gtattaaagc ctcattcagc agtatgttaa gcgggcatct cggggtgggt ataaccgtgg
      601 tgccggtgtg gagtccccgt ggaatgcggt ggccggtgtg aactggccgt tctgaccatc
      661 aacgattaac tgcgtttcgg cgctgttttc gtttacaggg tgctaaaagg gaaaattctg
      721 gggcaaaaaa agcccgccag ttacggcggg aaacctcatc ctattggaga acaatgaata
      781 atgaaattgc gggttatcat ctccctggat acccatgcta acaataaggt tatttactca
      841 accaggcgta aacattttgt tctgtgcgtg ggaacagcct taaggtgtaa agggggggga
      901 aatagcaatg agtagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg
      961 gatgaaaaac tcaaggcaga gatgactctg ccttgaagat aaatgcgctt ttacaggggg
     1021 cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc
     1081 catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa
     1141 tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac
     1201 ggcgttcagc aacaataatc tgcaaccgtt cttttgcaat gttggctgtg tttttcttcc
     1261 gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc
     1321 ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg
     1381 tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt
     1441 tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga
     1501 tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc
     1561 gcgttaacag caggtgctct ttaatcggct cttcgccatt ttctgcgcgg cgtgatttcg
     1621 acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg
     1681 taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg
     1741 gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga
     1801 ccccttcacg ggtgagggca tctttatcgc gagtttgcga tgccggaaga atatagagat
     1861 tttcagtacg tttatcttta attaacgcct ggtttagcgt tgcatcgccc tgaatgacgt
     1921 tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga ttacgcaggc
     1981 cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg
     2041 ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg
     2101 ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc
     2161 gactaactgc agtcgtgccg ctttgccata aaattctgct gggatttgat cactcagcca
     2221 gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc
     2281 ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga catgaatgtt
     2341 accatcggca atcaattcgg cccctgcgct aacgtggctt gtaacaatca gatcacattg
     2401 tggagcataa atacgctgac cggaacgcac tggggtatca attaaacgcg tttttgtgac
     2461 cggcgttgta ttttgcgctg gagcctgcgg tgcgggggct ggacgcggcg atttttcctt
     2521 tccttccgtc aggataggca gccccatctt ttcaatttcg gctttaagtt gcgcatcttt
     2581 gcagccactt acgccaataa cccgcaaacc agttgccgaa accgccttat gcatcgccga
     2641 ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa
     2701 tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc
     2761 atgcagatga accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga
     2821 catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga
     2881 ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga
     2941 gtaaaagtct attctgtgat aaatggcgtt gattcatagc ttaaaaaata cccttgtcaa
     3001 tcaacccatt gccgtcgtac ttttgattgt tcttatttac gcttcctttt tccgcaccct
     3061 aatcgtgaca gcatcttcag acctgtttaa atatccagta ttgaggcgag tgtatttttt
     3121 caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga acagataccc
     3181 gttatcttaa tatccgccgc agaattgctc tatcatagaa accgcagaag acgtctgata
     3241 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga
     3301 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3361 ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggaaatgac
     3421 gctggcgggc ttcaatctcc tgcgtgtggc gttgtttgcg acccgtatat ttgtgtgagt
     3481 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3541 ttacaatcat tacaaggcta cgatcccagc gaatttacat tctctaacgg tgtattttgc
     3601 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtggatgg taagcggagt
     3661 ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt
     3721 tacattatgt tctattcgtc attaacctct taagcgacag agaggagtgt ggcgttaacg
     3781 tttatgacgg ggagagtccc cgtcggttga cattt
//
LOCUS       DAPQZD010000279         3756 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPQZD010000279 DAPQZD010000000
VERSION     DAPQZD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.285"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            5..1390
                     /gene="dcuC"
                     /locus_tag="RO454_004857"
     CDS             5..1390
                     /gene="dcuC"
                     /locus_tag="RO454_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDY0936550.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1431..2111)
                     /gene="dpiA"
                     /locus_tag="RO454_004858"
                     /gene_synonym="citB"
     CDS             complement(1431..2111)
                     /gene="dpiA"
                     /locus_tag="RO454_004858"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDY0936551.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2080..3738)
                     /gene="dpiB"
                     /locus_tag="RO454_004859"
     CDS             complement(2080..3738)
                     /gene="dpiB"
                     /locus_tag="RO454_004859"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDY0936552.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          860 a          985 c          909 g         1002 t
ORIGIN      
        1 aattatgctg acattcattg aactccttat tggggttgtg gttattgtgg gtgtagctcg
       61 ctacatcatt aaagggtatt ctgccactgg cgtgttattt gtcggtggcc tgttattgct
      121 gattatcagt gccattatgg ggcacaaagt gttaccgtcc agccaggctt caacaggcta
      181 cagcgccacg gatatcgttg aatacgttaa aatattgcta atgagccgcg gcggcgacct
      241 cggcatgatg attatgatgc tgtgtggctt tgccgcttac atgacccata tcggcgcgaa
      301 tgatatggtg gtcaagctgg cgtcaaaacc attgcagtat attaactccc cttaccttct
      361 gatgattgcc gcctattttg ttgcctgtct gatgtcactg gccgtctctt ccgcaaccgg
      421 tctgggtgtt ttgctgatgg caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg
      481 tggcgcagct gctgccattt gtgcctcccc ggcggcgatt attctcgcac cgacttcagg
      541 ggatgtggtg ctggcagcgc aggcttccga aatgtcgctg attgacttcg ccttcaaaac
      601 aacgctgcct atctcaattg ctgcaattat cggcatggcg atcgcccact tcttctggca
      661 acgttatctg gataaaaaag agcacatctc tcatgaaatg ttagatgtca gtgaaatcac
      721 caccactgcc cctgcgtttt atgccatttt gccgttcacg ccgatcatcg gagtgctgat
      781 ttttgacggc aaatggggtc cgcaattaca catcatcact attctggtga tttgtatgct
      841 aattgcctcc attctggagt tcatccgcag ctttaatacc cagaaagttt tctctggtct
      901 ggaagtggct tatcgcggta tggcagatgc atttgctaac gtggtgatgc tgctggttgc
      961 cgctggggta ttcgctcagg ggcttagcac catcggcttt attcaaagtc tgatttctat
     1021 cgctacctcg tttggttcgg cgagtatcat cctgatgctg gtattggtga tcctgacaat
     1081 gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat
     1141 cccgaaactg gcgcactcct ccggcattaa cccggcgtat ttgactatcc cgatgctgca
     1201 ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc gtagtcgttg cggttgccgg
     1261 gatggcgaag atctcgccgt ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg
     1321 tttggtgatt gttatcgttg ctacagagct gatggtgcca ggaacggcag cagcggtcac
     1381 aggcaagtaa atagtaatgc cggaggcaag tttcctccgg ctttatcatg tcacccactg
     1441 tggtatatgc gttgcggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg
     1501 cggctggcgc aatattcaag ataacgcctg gcagtggtgc gactgatggt cagtgcctgc
     1561 gccaccgttt ctgccgtatg ttgcacacca ggctctttga acagttttcg caccgcgttt
     1621 agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg gttcaccgcg agcataggca
     1681 ttaaacatct catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta
     1741 cgctggcgga aacgggttag cgtttgcccc agacgttcat aggcaattgg cttaatgaga
     1801 taatcaaata caccacaacg tacggcttca gacaccgttt ccatatcgct ggctgcagtg
     1861 gtaaacacca cgtcgccggg ataatgcgcc tgcaccagtt catgcagtaa attaatccct
     1921 ctaccgtcag gaagatagtt atcgagcaag attagccccg gcttaaaacg ctcgatcatc
     1981 attcgggcct gcgccaggtt tcccgccagt aatatctggc tgaatccggg aatgtgacga
     2041 atatattccg catgcatctc tgccagcggc gtttcgtcct caacgatcaa tagggttaat
     2101 ggagctgtca ttaggtttca ctttcggaat atagattgaa aataaggtac cgcagggatc
     2161 attatcttcg agagtgataa caccaccgca gcgcgttacg tagctggcaa tcaagtacaa
     2221 cccaatgcca tgttcaccgg gctcgtcagc acgcgtactg acaccctgct caaatatttt
     2281 gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca ccacatcatc
     2341 gccttcatcg ctgaggtata attcaacaat cttgtttcct tcatcgctac gcaggcttgc
     2401 ttcgaaggcg ttatcaagta aattgccaac aatggctgca aactcggtgc tgtccagtcc
     2461 tggcggcagt tgcgaaagct ggctaccggg gacgatgacc atttttagcc ccagttcgcg
     2521 ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa acgcctcacg
     2581 caggctgtca ataagctgtt gctgggcctg agactcaccc tgcaccatcg ccagcacgcg
     2641 atcatactct ttcatctgca acagaccatt gagcgtcgac atccaattga gatgctcgtg
     2701 tcgcaatgta cgaaggctct cgacgtattg tttaatttgc gtcagttgcg cattgagggt
     2761 ggaaatttcg tctttactac gaaagctgat aatggccccc agcaaatcat cgccagaacg
     2821 aatagcttcc cgattggcga taacgctcag accgttaaag ttcgccacca catcctgacg
     2881 tttttcatca atctgttcgg taaagaaatc ggcgggcctg accacttcag caatgggttt
     2941 acccaaccat tgccgtccgg gggagctcag ccccagcatc tttcttgcgt tacgattgat
     3001 ggcggtaatg tacccgtgcg gatccaccgc aatcagccct tcataaaccg aactaaacag
     3061 cgcctcttgc tgacggacca cgcgtgcgat ttgctttggc tccatgccca tcatctgccg
     3121 acggatatgc gcggccagga accacgatag caacatcaaa atccctaaca gcacgacaaa
     3181 cacgcctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac tcaccagata
     3241 gccaatcgac accacaccga tgacttttcc atcgtcatca aagattggcg ttttggcacg
     3301 catcgccatg ccaattgacc ctttcccggt gatgaagtag ctctcccctt tctccagcgc
     3361 gcccggtttg gtgaactgca taggataacc aattttctcc ggattaggat ggtaaagacg
     3421 aatcgagtgc cggtcgccaa tcaccacata atcgaaatca gtatctcttt gtaatttgtc
     3481 agcgatggtc gccagcctct tgtagtcacg cgttttcact gcgctgatga tactgtcatt
     3541 agaagcaata atttttgcct gattcattgc catgtcgcgc acatgaagcg ttaaatagtc
     3601 ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg agaacaccag
     3661 aatcagcaaa aagatacgca gcggaaatgc cagtctttgg aaaaatgcaa actgtttatt
     3721 ctcgttaagc tgcaacattg ttttccttgc gggttg
//
LOCUS       DAPQZD010000280         3708 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPQZD010000280 DAPQZD010000000
VERSION     DAPQZD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.286"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1328
                     /gene="vgrG"
                     /locus_tag="RO454_004860"
     CDS             <1..1328
                     /gene="vgrG"
                     /locus_tag="RO454_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDY0936553.1"
                     /translation="RKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVM
                     AGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYG
                     LHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRL
                     DDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISA
                     DAQDKAQGQVREMADIISELNSLSDKIQKLSDDAATANADPADMAAQVALITSRINDL
                     TASVILMHAPKGVAVASGEHLQLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVR
                     KAGIKLIANKGAVSVQAQHDLMELLAKKSIEIVSTEDEIRISAKKKITINGGGSYIRI
                     EGSGIEPGTPGDYNVKAVHYGRMGKAHEPVELQMLAEKVDEPPVKFFFS"
     gene            1340..3703
                     /locus_tag="RO454_004861"
     CDS             1340..3703
                     /locus_tag="RO454_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HDY0936554.1"
                     /translation="MIISPPFIRAKNNNENDAHWIERMMPVEPDRDYPINYGGSWHGG
                     IHVRHTNSDRQPEYVRAIADGVVVSIRNPSDQAACSLPPLNYNGSTDDGYVLLRHETE
                     IGTGEDGNIVYYSLYMHLEKIEEGISEGNKVYRKSSLGMPGHVDGDRGLHFQIFCDDD
                     NLRKITGRTTPEMDVSRHGRTDVVYGDIYFYLPPGTRFYRSAPENNAVDNTGEVFQST
                     EPLVVTMCFDTGHCIMTTRRANPVIPSRYDIVGEVLTDADGTDYEYNLYSAALRLYPQ
                     SPSAGFELLRFGRVINTEHETLIPANAPLWRTVSYPGGQGVINLAADVVTKFSDGDFP
                     HWSGWRLVDDDADSNSQCNSVILRGGGFTDFRQMICHFPLEWCNTGIEARYGWLKEGV
                     IQERQENEANSDLSLGTDYYRPNNVEYNTTGDSHGESPQSAISEMPDLSYEQSNAYEN
                     AVRVLLLGEEDWQTLVKHIKALCFDTSSIAEGRVWHFNPGEFIKHFRKCGWVDKAILK
                     RIFASGTNDRDRAVIEQKVDIYGTAINIVMNKYIMNSPLRRAHFLGQGAVESSRLRSM
                     QEKSQYQTVDENGRPVGGGIVPDSLRDENSDLGHWYGAIETEVDGYFSGVKYNSGGGR
                     IAGSYDWILGNCDTEDAQKFRGRGFKQLTGRSNYAEYWVYRAWIDTNSFTAKWWEDPL
                     WRLHDRRRLTRIPANIEEPHRVTRSEYNCIDTGGFFIVKTVDRRGTRSSITRAMDQDS
                     EVIHLNNTTIDTTDEQTIRSVSRAINGGENGLPDRITFTRAAKKVLI"
BASE COUNT         1021 a          784 c         1044 g          859 t
ORIGIN      
        1 tccgcaaggg cgtactgatt accggcgtga cggccagcgc ggcgcgtgac cgcagctatg
       61 agctgacgtt taccgccatc ccgtactcag agcgctacgg ctaccgcccg gcgcttatcc
      121 cccgcccggt aatggccggc acactcccgg cgcgggtcac cagcacggtg aaaaacgata
      181 tctatgccca tatcgacaag gacgggcgct accgggtcaa cctcgacttt gaccgcgata
      241 cgtggaaacc gggttatgaa agcctgtggg tgcgccagtc gcgcccgtat gcaggtgaca
      301 cgtatggcct gcatctgccg ctgctggcgg gaacggaagt cagcatcgcc tttgaggaag
      361 gcaacccgga ccgaccttat atcgccgggg taaaacacga ctcggcacac accgaccatg
      421 tgaccattca gaactacaag cgtaacgtgc tgcgcacccc ggcgaacaac aaaatccgcc
      481 tcgacgatga gcgcggcaaa gagcatatca aggtcagtac cgagtacggc ggcaagagcc
      541 agctaaacct cggccatctg gtggatgccg gaaaacagca gcgcggggaa ggttttgaac
      601 tgcgtaccga tctgtggggt gcagtacggg caaagaaagg catctttatc agcgccgacg
      661 cgcaggataa ggcgcagggt caggtacgcg aaatggccga cattatcagc gaactgaaca
      721 gcctgagcga taaaatccag aaactgtctg acgatgcggc cacggcgaac gccgatccgg
      781 cggatatggc cgcgcaggtg gcgctgataa ccagccggat aaatgacctg accgcctcgg
      841 tgattttgat gcacgcgccg aaaggtgtgg cggtcgccag cggcgagcac ctgcaactgg
      901 ccgcagtgaa aaacctccag ataaatgcgg gaaacaacgc cgatatcggc gtggtgaaga
      961 atatgtttat cggcgtaggg cgggcgctga gcgtgtttgt gcgcaaggcg ggtatcaaac
     1021 tgattgccaa taaaggcgca gtatcggtac aggcgcagca cgatctgatg gagttactgg
     1081 cgaagaagtc gatagagatc gtcagtactg aggacgagat caggatttca gcgaagaaaa
     1141 aaataacgat taatggtggt ggaagttata tccggattga gggtagcggc attgagccgg
     1201 gaacgccggg ggattataac gtcaaagcgg tgcattacgg gcgtatggga aaagcacatg
     1261 agcctgttga gttacaaatg ttggcagaga aagtagatga gccacccgta aaatttttct
     1321 tttcttaagg atataagaaa tgattattag cccgccattt atacgagcga aaaataacaa
     1381 tgaaaacgat gctcattgga ttgaaaggat gatgccagtt gaacctgacc gtgactatcc
     1441 catcaactat gggggatcat ggcatggagg catccacgtt agacatacaa acagtgacag
     1501 gcaaccagaa tatgtccgtg ccattgctga tggtgtcgtg gtatctattc gcaatccctc
     1561 tgaccaggca gcatgtagtc ttccgccgct caattataat ggaagcacag acgatgggta
     1621 tgttctgctt cgtcatgaaa ctgaaatagg cactggtgag gacggcaata ttgtttatta
     1681 ctctctttac atgcacctgg aaaagattga agagggtatc agtgaaggaa ataaagtcta
     1741 ccggaaatcg tcactgggaa tgccggggca tgtcgacggg gatcgcggcc tgcacttcca
     1801 aattttctgc gatgatgaca atctgagaaa aataaccggg cgcactacac ctgagatgga
     1861 tgtgagccgt catgggcgta ccgacgtggt ttacggggat atctattttt acctgccgcc
     1921 cggtacccgc ttttacagga gtgcgccgga aaacaacgca gtagataaca caggtgaagt
     1981 ttttcagagc acggaaccgt tggttgtcac catgtgtttt gatactggcc attgtatcat
     2041 gactacccgc agagcaaatc cggtgatacc aagccgttat gacattgtgg gagaagtgtt
     2101 gacggatgct gacggcactg attatgaata caacttgtac agtgctgcac tgagacttta
     2161 tccgcaaagt ccaagcgcag ggtttgagtt actgcgcttt ggtcgggtta tcaataccga
     2221 gcatgaaaca ctgatacccg cgaatgcgcc actgtggcga accgtgagct atcccggtgg
     2281 gcaaggagtg ataaatctgg cggcagatgt tgtgacaaaa tttagcgacg gtgattttcc
     2341 gcactggagt ggctggcgac tggtagatga cgacgctgac agcaacagtc agtgcaattc
     2401 agtaatatta cgtggtggtg gttttacgga ttttcgtcag atgatttgcc acttcccact
     2461 ggagtggtgt aatacgggaa tagaagcgcg ttacggctgg ttgaaagaag gggtaataca
     2521 ggaacgtcag gaaaatgagg ctaacagcga tttatcatta ggtacggatt actacaggcc
     2581 aaataatgtc gaatataata cgactggaga tagccatgga gaatctcctc agtcagctat
     2641 atcagaaatg ccagatttgt cttatgaaca gtcaaatgct tatgaaaatg ccgtcagagt
     2701 tttattactg ggagaagaag actggcagac gttggtgaag catattaagg cactgtgttt
     2761 tgataccagt agtattgctg aaggacgggt ttggcatttt aatccgggtg agtttattaa
     2821 gcattttagg aagtgtgggt gggttgacaa agcaatattg aagcgaattt ttgcctctgg
     2881 tactaatgac cgtgatagag cggttattga acaaaaagtt gatatatatg gtacggctat
     2941 aaatattgtt atgaataaat atataatgaa ttctcctctg agacgtgctc actttctggg
     3001 gcaaggggca gtggaatctt cccgtttaag aagtatgcag gaaaaaagcc agtatcaaac
     3061 tgttgatgaa aatggtcggc cagtcggcgg aggtattgtt ccggactcat tacgtgatga
     3121 gaattcggac ttgggtcatt ggtatggtgc aattgagact gaggtcgatg gatacttctc
     3181 tggtgttaaa tataattctg gtggaggacg aattgcaggc tcatatgact ggatactagg
     3241 aaactgcgac actgaggatg cccagaagtt tcgtgggcga ggttttaagc agttaactgg
     3301 tcgatcaaat tatgccgaat attgggtcta tcgtgcctgg atagatacga atagttttac
     3361 cgctaagtgg tgggaagacc ctctgtggcg cttacatgat cgtcgtcgtt taactcgcat
     3421 accggctaat atcgaagagc ctcacagggt taccagaagc gaatataact gtatcgatac
     3481 tggtggattc tttattgtta agaccgttga tcgtcggggt actcggtcaa gtattaccag
     3541 agcgatggat caagacagtg aagttattca tttgaacaat accacgatag atacaacgga
     3601 tgaacaaaca ataagaagtg tgtcccgtgc aattaatggt ggtgaaaatg gtttgccaga
     3661 caggataacc ttcacacgcg cagctaagaa ggttttaata tgaaaaag
//
LOCUS       DAPQZD010000281         3678 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPQZD010000281 DAPQZD010000000
VERSION     DAPQZD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.287"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..492
                     /locus_tag="RO454_004862"
     CDS             <1..492
                     /locus_tag="RO454_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936555.1"
                     /translation="SKTSFHLMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISA
                     DIKTNVRDLVDFQSSISHVKLFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDD
                     GLYHYTSDLTGTPLLRLKNTVKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINW
                     CNP"
     gene            489..1004
                     /locus_tag="RO454_004863"
     CDS             489..1004
                     /locus_tag="RO454_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936556.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            1006..2892
                     /locus_tag="RO454_004864"
     CDS             1006..2892
                     /locus_tag="RO454_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936557.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRYKSKESLDG
                     MKNQIKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQKDIEILLEKSS
                     QPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVDGQPRQSHGKG
                     VRAILYSAFIIGLLKYCIKKGYPHPGLVVIDSPLTSYKKKAAATVSGFDEPISAGIEE
                     GFWHSLTQLPDTLQIIVIENKEPPVSIMNKVHYEWFAGEHAMDGERRALIPLPSKNSA
                     ILMK"
BASE COUNT         1075 a          730 c          869 g         1004 t
ORIGIN      
        1 agtaaaacct cttttcatct tatggtcttt ggcgcaccat taattgagcg ggatccacca
       61 actaggcccc catcatctgt ggctcccatt gaaactgtat atattgaaca actcttttca
      121 gttatcagcg ctgatatcaa aaccaacgtg cgagatttag ttgattttca atcaagtatc
      181 agccatgtaa aactctttga acggtcccga ataacttttt attgctcgga agggctaaag
      241 gaactcgcta gggaccaaat ggcgaatcaa gaattcttta attctttact tgtagagttt
      301 gatgatggtc tgtatcacta cacctctgac ctgacaggga ctccactcct tcggttaaaa
      361 aataccgtca aggccgctca gaccctgcaa cttggtgcgc acccacttgc cattcacgta
      421 acgaataagg atcgtgaagg tatatgccac caactagcca atacaaactt gattaattgg
      481 tgcaatccat gagcgaaatg caagtatttg aaggaaactt tgagcagaga ccttttaaca
      541 ctccgctgga gtatggattt cgtgctctgt tcatattgga tgcagcggac aatgtagcaa
      601 tggacctaca aaggatagtt tcgtatgact atcttcttgt gcataccagt gacgtaccta
      661 gggggcctaa aagccttcat ccagcagttc ctcaccgggg agcagagctt cttgttaagc
      721 gaagcgcaat tcaggcaggg ctgcatttaa tgctatcaag agaattgatt aaggttgtct
      781 ttgctgccga aggtattttg tatcaggcta ccaatttaac aggaaggttt gtgcggcttt
      841 tgatgtcaca gtattcaaaa gagctcgcag aacgtgcctc ttgggtcaca aagcaattct
      901 atgaatatac ggatgaagaa cttgcttcat atatatctca gaatgtgggg caatggggtt
      961 ctgaattcga ccgattgacc gcaattgatc tactggattt ataacatgct tcgtataaga
     1021 aaaatactat tgcgcggaaa cagcgttcaa gacgcttatg ttgatttcta taagggtgcg
     1081 aatattctag caggggaatc tgatactggg aaaagttacc ttgtgagttg tcttgactac
     1141 attcttggtg ccgaaaaact caaaaaaaag cttaaagagg ccaccgatta cacccatctt
     1201 tatgttgaat tcgaaaatga tgaagggggg gtgttaacgc tcaagcgcgg tcttgagggg
     1261 gggaaattag aggcacatga tgtcgctatt caagacatcc atggagaagg taaaataatt
     1321 gctcctgtgc gtaaaggaac gagtaagggg ccagatgtga cgtccatact tttccctttt
     1381 gcggggatca aagaggcgaa gcttcgcaag aatgctcgtg gcgaaacgca acgtttctcg
     1441 attcgaacgc tcgctcctat atttttggta gatgaagtat ccattatcga cgaatactcg
     1501 ccagtaacgg gacgttccgg ttatgacgac actgctcgaa aacgcatgtt ttcatacatc
     1561 cttaccgggc acgatgatgg tggtgttacc gtagaggaaa agcctgaaat tgtaaaagcg
     1621 aggttgatgg cgaagcttga attcatccag gatttgattc gaccacttga tgaacgtttc
     1681 tcaatttgtt cgcccaagtt tccattaacg agctcagcag atgatttatc agatcaattg
     1741 attgcacaag caattgatga agttgagagg gctgcagccg ctatttctga cttgctcgag
     1801 ggaataaaaa tggagacagc gttaacgtta aaaatagaat ctcagttaat gggagtatct
     1861 gaaattcaat cgagatatag cctattagaa gaacgttacc attccgacct gaagcggttg
     1921 gattttattt ccgaaggttc tcactatttc acttcacttc aggaagttcc ttgttccctt
     1981 tgtggacaaa atttactaca tccacacagt gagaacgcta aaaaattaat gaacagtaat
     2041 gaagtccgac gttcttcttt agctgaagct gcaaaaattc atggatacct tgcaggcctg
     2101 caaaaagcga tgagtgatct tgaccgaaga aaagaagctc tgaatataga cagatataag
     2161 tcaaaagaaa gcttggatgg aatgaagaac cagatcaaat atacttttga gcctcttctt
     2221 acactttcca aagaaagcct agatttagct attgagcagc gagctgaaag agactctgag
     2281 ttgaatgatc gacagagatg gtttgagtta ctaaaacatc aaaaggatat agaaatactg
     2341 ctggaaaaga gcagtcaacc acggctcgaa tgggagggat tatctacccg tacgctggcc
     2401 gaactttgta gagaaattga aactgtttta aaagactgga aatggggtgc cgagcctgac
     2461 gtttcattta atgaaaaaga gtatgacatc attgttgatg gacaacctcg gcagtctcac
     2521 ggtaaaggtg ttcgtgccat tctttattca gctttcatca ttggattact aaaatattgt
     2581 attaaaaaag gatacccgca tcctgggcta gtcgtgattg attcaccgct gacaagttat
     2641 aagaagaaag ccgctgctac ggtatcgggc ttcgatgaac ccatatcggc tggtattgag
     2701 gaaggcttct ggcattcact gacccaattg cctgatacct tacaaatcat agttatagag
     2761 aataaagaac cgccggtatc aatcatgaat aaagttcatt acgaatggtt tgcgggtgaa
     2821 catgcaatgg atggagagcg cagagccctt atcccgctgc cctcaaaaaa tagtgctatt
     2881 ttaatgaagt aacttaggat gggtagccat caaatcgcag ttatacgatt ggtggggttt
     2941 gtaaaagcgg tagtcttgat taccactgca ttcattctac aagcacatac ttccctaggt
     3001 tgctgtagtt atccgattac acgccttttc tgaaagcagc ctcagggagc cggataaaac
     3061 ccgttaaaag tggcttgcct atactgctgc gcggcgctga cgctttagag gccccaggat
     3121 ttaggcaaat caatgtagtt agctcagtag caggtcatat ctgggctata aatggcatta
     3181 cgtgacactg aaagagcttc ccagtggcat cgtatttggt gagccccaac ctacaagacg
     3241 ggcggggata gccgtgattg gcacccacag caaggcgata aagatgttca acggagcggc
     3301 tcagcaggta tggggatggt aggatttagt cttcatatgc ctgtggaatt aatcaaccct
     3361 cagagcccga cacacccggc attgcttacg gtataagact gattgagaag gtgtttgacg
     3421 tttttgtatc ccagtctgtc ggtaaagtaa acacagtgat cagtaactgg tagtactgga
     3481 agtgattgcg gtcgtaatgc tgattttgca cgctacgaca ggtatattct gtttggtatc
     3541 tgtagaggtt gtgatggcgc tgaccttgca catgccaggt acgttagact taataataaa
     3601 gggagtatta gcaggaggga ttttttcgga ctcagttgta ttgtgtgtaa cgagccaact
     3661 aaatgtgata atagctat
//
LOCUS       DAPQZD010000282         3643 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPQZD010000282 DAPQZD010000000
VERSION     DAPQZD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.288"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            261..2195
                     /gene="yeaG"
                     /locus_tag="RO454_004865"
     CDS             261..2195
                     /gene="yeaG"
                     /locus_tag="RO454_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDY0936558.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2308..3591
                     /locus_tag="RO454_004866"
     CDS             2308..3591
                     /locus_tag="RO454_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDY0936559.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT          983 a          880 c          913 g          867 t
ORIGIN      
        1 cattaattat gcaaaattta tggatgagtt gttgatatgc cattgaaatt aagaaagccg
       61 tgcaggcaag ttttccattt gccatctacg cttaattttg aaggtgtatc accgggcacg
      121 ttgttctcat cgtcaataaa atggcatgag agttgctgtg ttttagcaag agacgtcgtt
      181 cagtttacct cttccgggag cctctactat tcatatgaac ggctcttaac ctgtgctaaa
      241 aaacgaaagg acggcatacc atgaatatat tcgatcacta tcgccagcga tatgaagctg
      301 ccaaggacga agagttcaca ctgcaggagt ttcttaccac ttgtcggcaa gatcgcagtg
      361 cttatgccaa cgcggctgag cggctattga tggctattgg tgagcctgtc atggtcgata
      421 cagcccagga acccagactt tctcgactct tttctaaccg ggtcattgca cgttatccgg
      481 cgtttgaaga gttttacggc atggaagacg cgattgaaca gattgtttct tatctgaaac
      541 acgcggctca ggggctggaa gagaagaaac aaattctgta tctgctgggg cctgtgggtg
      601 ggggtaaatc atcacttgct gagcgactga aatcattaat gcagctcgta ccgatttatg
      661 tattgagcgc caacggtgag cgtagcccgg tcaacgatca tccgttctgt cttttcaatc
      721 cgcaggaaga tgcgcagatt ctggaaaaag agtacggcat tcctcgccgt tatctcggca
      781 ccatcatgtc gccgtgggcg gcaaagcgcc tgcatgaatt tggtggcgat atcactaagt
      841 tccgggtagt gaaggtctgg ccgtcaattc tgcaacaaat tgccatcgcc aaaacggaac
      901 ccggagatga gaacaaccag gacatctccg ccctggtagg gaaagtcgat attcgtaaac
      961 tcgaacacta cgcgcagaat gacccggacg cctacggcta ttccggtgcg ctgtgccgcg
     1021 ccaatcaggg gatcatggaa tttgtcgaga tgtttaaagc accgataaaa gtgctgcatc
     1081 ccttgttaac cgccacccag gaaggtaact acaacgggac ggaaggtatc tccgccctgc
     1141 cgttcaacgg gattattctc gcccactcga acgaatccga atgggtcact ttccgtaata
     1201 acaaaaacaa cgaagccttc ctcgaccgtg tttacatcgt gaaggtgccg tattgcttgc
     1261 gcatttccga agagatcaaa atctacgaga aattgcttaa tcacagtgaa ttgactcacg
     1321 ccccctgcgc ccctggcacg ctggaaacgc tgtcacgttt ttctattctt tcgcgtctga
     1381 aagagccaga aaactccagc atttattcaa agatgcgcgt ttatgatggt gaaagtctga
     1441 aagacactga tcccaaagcc aagtcgtatc aggaatatcg tgactacgcc ggtgtcgatg
     1501 aagggatgaa cggtctgtcg acgcgttttg cgtttaagat cctctcccgc gtgttcaact
     1561 tcgatcatgt agaagtggcg gcaaacccgg tccatctgtt ctacgtcctg gaacagcaga
     1621 tcgagcgcga gcagttcccg caagagcagg ctgaacgcta tctggagttc ctgaaaggtt
     1681 atctgatccc gaaatatgcc gagtttatcg gcaaagagat ccagacggcc taccttgaat
     1741 cctattcaga atatgggcaa aacattttcg accgttatgt tacctacgcg gatttctgga
     1801 ttcaggatca ggagtatcgc gatccggata ccgggcagct gtttgaccgc gagtctctta
     1861 acgccgagct ggagaaaatc gagaaaccgg cggggatcag taatccaaaa gatttccgca
     1921 acgagattgt taacttcgta ctgcgagcca gagcgaataa cagcggacgc aatccgaact
     1981 ggaccagcta tgaaaaactg cgcacggtta tcgagaagaa aatgttctcc aataccgaag
     2041 agctgttgcc ggttatttcg tttaacgcca aaacgtcaac cgacgagcag aagaaacacg
     2101 acgactttgt cgaccgtatg atggaaaaag gctacacccg taaacaggtg cgtttactgt
     2161 gcgaatggta tttgcgcgta cgtaaatcgt cttaacaacc ctggcccggt acacatgcta
     2221 ccgggcctac aacgacagcg aaccgtgggc cagagaagcg gcaacacaag cgtagcatac
     2281 cgttggcaaa tgtagtacgg ggggcatatg acctggttta ttgaccggcg tctgaacggc
     2341 aaaaataaaa gcatggtgaa tcgccagcgt ttcttacgcc gttataaagc gcaaattaaa
     2401 cagtcgatct ccgaggccat taataagcgt tcggtgactg acgtcgacag cggcgagtcc
     2461 gtatccattc ccacggaaga tattagcgaa ccgatgtttc atcaggggcg tgggggcctg
     2521 cgccaccgtg tgcatccggg caatgaccat ttcgtccaga acgaccgaat tgaacgtccc
     2581 cagggtggtg gcggaggttc cggcagtggt cagggccagg ccagccagga tggtgaaggt
     2641 caggatgaat ttgtctttca gatttcgaaa gatgagtatc ttgatctgct ctttgaagat
     2701 ttggccttac cgaatctgaa acaaaaccaa caacgccagc tgaccgaata taaaacgcat
     2761 cgggcgggtt ataccgctaa cggcgttccg gccaatatca gcgttgtacg ttcattgcag
     2821 aactcactgg cgcggcgcac agctatgacg gcgggcaagc ggcgggaact tcatgcgctg
     2881 gaagagaatt tggccatcat cagcaacagt gaacctgcgc aactgctgga agaggaacgt
     2941 ctgcgcaaag aaattgcaga attacgtgcc aaaattgaac gcgtcccttt cattgacacc
     3001 ttcgatttac gttacaagaa ctacgaaaaa cggcccgatc cctccagcca ggcagtgatg
     3061 ttttgcctga tggacgtttc cggttcaatg gatcagtcca ctaaagatat ggctaaacgt
     3121 ttttatattc tgctgtatct gttcctcagc agaacgtata agaacgtgga agtcgtatac
     3181 atccgccatc atacccaggc gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa
     3241 actggcggca ccattgtttc cagcgcccta aaactgatgg atgaggtagt gaaagagcgt
     3301 tataacccgg cacagtggaa tatttacgca gcccaagcat cagacggcga taactgggcc
     3361 gatgactctc cgctttgcca tgaaattctg gcgaaaaaaa tattacccgt tgttcgttat
     3421 tacagctata tcgaaattac acgtcgcgcg catcagacac tgtggcgaga atatgagcat
     3481 ctgcaatcta ctttcgacaa ctttgcgatg cagcatatcc gcgaccagga tgatatttat
     3541 ccggtgttcc gtgaactgtt tcataaacaa aatgcaacag ctaaagacta aaactgtcag
     3601 ccgggtcatt atcgcctggc tgatttttag ctcactgtaa att
//
LOCUS       DAPQZD010000283         3614 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPQZD010000283 DAPQZD010000000
VERSION     DAPQZD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.289"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            102..425
                     /locus_tag="RO454_004867"
     CDS             102..425
                     /locus_tag="RO454_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDY0936560.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            597..1094
                     /gene="ftnA"
                     /locus_tag="RO454_004868"
     CDS             597..1094
                     /gene="ftnA"
                     /locus_tag="RO454_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDY0936561.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1132..1371)
                     /locus_tag="RO454_004869"
     CDS             complement(1132..1371)
                     /locus_tag="RO454_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDY0936562.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1562..2773
                     /gene="tyrP"
                     /locus_tag="RO454_004870"
     CDS             1562..2773
                     /gene="tyrP"
                     /locus_tag="RO454_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDY0936563.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2824..3489)
                     /gene="yecA"
                     /locus_tag="RO454_004871"
     CDS             complement(2824..3489)
                     /gene="yecA"
                     /locus_tag="RO454_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDY0936564.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT          833 a          894 c          858 g         1029 t
ORIGIN      
        1 acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
       61 acgcgtaaaa tactgctttc ttttactctt ctggctggac catgagactt ctgattctga
      121 ctctttcctt aataacgctg acggggtgta cggttactcg ccaggcccat gtgagcgaag
      181 ttgacgcggc aacaggtatt gttcggttgg tttatgatca ggcttttttg cagcatgccc
      241 gtaccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gcaggctata
      301 cccacgctat cccctttggt cagccagtag gcaactgtag cctctttgcg ggttccttat
      361 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
      421 tctaaacgtt ttaatcagcg agggggatct tcgctgatta aataaatagc ggtaatgcgt
      481 tttattccca ttcgtatttt taataaatga tgtttatatt ttaccttttg caaataataa
      541 aataacaaat tatagtgacg ctacagcaac aaatataacc tttgtggagc actatcatgc
      601 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
      661 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
      721 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
      781 ctgataccgg caacttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
      841 cacttgatga attattccag gaaacctata aacacgaaca attaatcacg cagaaaatta
      901 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcaat
      961 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
     1021 gcctggcagg caaaagtggc gaaggtctgt attttatcga caaagaactc tctaccctcg
     1081 acacacaaaa ctaatgctct ccggcggcag gttttctgcc gccgtttcaa atcagtgacg
     1141 gcaaatctta ctctgatcgg ttgaaaaacc ttcttctgaa ggtataaatt tgccttttgc
     1201 tgccagaaac gccaccaact ctcccgcagt catcccttct gccgagcagg tgtgaaaacg
     1261 tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga ctggcatgcg tatattgctc
     1321 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc
     1381 tcaaaaaaag cctagcgtag cgattgctgc ttatgaagac tttgcgccag cgcagaactg
     1441 aacgcttttt attgtacatt tatatttaca ctatatgtaa cgtcgatttg acgaatcagc
     1501 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
     1561 cgtgaaaaac agaactctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
     1621 catgctggca atgccgctgg ctgcggcagg tgttggtttt agcgtcacgt taatcttgtt
     1681 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtatcagca
     1741 tgtcccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
     1801 gtggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
     1861 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
     1921 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg tcggaacttc
     1981 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
     2041 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
     2101 gggactggca ctgtctgcaa tcccggtgat ttttacgtcg tttggttttc acggtagcgt
     2161 gccgagtatt gtcagctata tggatggcaa cattcgtaag ctacgctggg tgtttataat
     2221 cggtagtgcg atccccctgg tggcatatat tttctggcag ctggcgaccc ttggcagcat
     2281 tgattcaaca acctttatgg gattgctggc taatcatgcc ggattaaacg ggctgttaca
     2341 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
     2401 tttagccctc gccacgtcct tcctcggcgt agcgttaggc ttatttgatt atctggcaga
     2461 tttgtttcag cgttcaaata ccgttggtgg acgattgcaa actggtgcaa ttacctttct
     2521 gccgccactg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
     2581 cggtgtggca ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
     2641 gaagcacaat cctcaggcgg gttaccgggt gaaaggtggt cgtccggcgc tggtggtggt
     2701 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag gcttgttacc
     2761 agaagtgggg tgattagatt cttattgggt gccagaatta acgctgacac ccaatttgac
     2821 ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc aaggatcatt
     2881 acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc
     2941 catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa
     3001 cgcttctggc gacatcttct ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat
     3061 cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa gtgccacgcc
     3121 ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat cgacttctcg
     3181 caagccgaat aatggctcaa actgctccgg aaattcgttc agacgctctg cggtatcggc
     3241 catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccagcgcgg
     3301 cacatagtca gccccacccc acacggcaac cagccactgt gccggttcaa tctcttgcgg
     3361 agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagaa tggcgtggtc
     3421 agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc
     3481 cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt
     3541 ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa
     3601 cgacaagttg cagg
//
LOCUS       DAPQZD010000284         3607 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPQZD010000284 DAPQZD010000000
VERSION     DAPQZD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.290"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(131..388)
                     /gene="repA"
                     /locus_tag="RO454_004872"
     CDS             complement(131..388)
                     /gene="repA"
                     /locus_tag="RO454_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDY0936565.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNCLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            793..1815
                     /locus_tag="RO454_004873"
     CDS             793..1815
                     /locus_tag="RO454_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDY0936566.1"
                     /translation="MTITTIGIDLAKNVFAVHGVDQNGKTVLVKSRVTRAALPGLIAS
                     LPPCVIGMEACSGAYYWARLFLQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICE
                     AVRRPHMRFVPVKDESQQAMQCLHRNRQGFIEEKTATYNRLRGLISEFGVIAPQSTDA
                     LRHMVSAQKSSLPLQVQQCVDDLLEHVDRIEANITEYDRILSRIAKTDHRSQRLMKLK
                     GVGPTTACALVACIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRT
                     LLVQGARSVLIGAEKRTDLFSRWVCSLVERRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSAS"
     gene            2287..2640
                     /locus_tag="RO454_004874"
     CDS             2287..2640
                     /locus_tag="RO454_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936567.1"
                     /translation="MSETVQIESFNAEFLTKQKLHDLIFYRKSFDITNVNDISETVKI
                     VEYVIEHQQKKLTCRVYTEYRSTAVAGSLWSPTALLGAVSAAAIGVHNLATWNPDYEI
                     GKNYVKRTISVRYKK"
     gene            complement(3053..3607)
                     /locus_tag="RO454_004875"
     CDS             complement(3053..3607)
                     /locus_tag="RO454_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDY0936568.1"
                     /translation="MAALLIRIILRSVNQHSPLLMQLHEAGIRTGDAERILSSGECWQ
                     RQKTLLTGREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMV
                     SQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDAR
                     KLLQMTGEWLNTTGADQQSPEHRS"
BASE COUNT          973 a          867 c          832 g          935 t
ORIGIN      
        1 cttttctggc tcctgtaaat ccacatcaga accagttccc tgccacctta cggcgtggcc
       61 agccacaaaa ttccttaaaa cgatcagtaa tctagcactg acagaaaaaa ctagcaagaa
      121 tgtgacccca tcacatcatg ttccggtgac aggcctcttt ctcgataagc ctttctatca
      181 actctgcctg agttacccct tcctctgcac acagactggt aaggcagttt ttcagttctg
      241 gtctgacgaa aaccttgatc tctttacata cagatcgttt gcgcgcagaa gctgctctct
      301 gtcgttctgc ttcactcaaa ggtttaccct ttctgtatgc tcgttttgca ccagatgagg
      361 aagtcactgc attttctgtc tgcgacatct cgcctcctca atactcaaac agggatcgtt
      421 tcgcagagaa tactacagtt ttttgaaatc agcgacttga gaattgtgac gaagatctgt
      481 gttggcgcac aaatcaacgg ggattactgt cgtttaaggg ctgcgtttgg gaaacgtaca
      541 atattgtacg gttgcgttat cgcgctcgga cgctatacgg tgtttggcag acctttaggg
      601 acatgagata acctaaatgc gataagagaa aagcaataac ttaaaggcga tagctgatca
      661 cgatgaatca atcagttact tatcagtatc ggttaaaaaa cgcccctaac gaatggaagg
      721 gacttcccct ggatatgcac ctgattaact cttgtgcaat catggggtaa acatagcctg
      781 gagagattcg ttatgaccat cactactatc ggtatcgatc ttgctaaaaa tgtgttcgct
      841 gttcatgggg ttgaccaaaa tggcaaaacc gttctggtga aatccagagt aacccgcgca
      901 gctcttcccg ggctgattgc aagtttaccg ccctgtgtta tcgggatgga ggcgtgctcc
      961 ggggcttatt actgggcaag attgtttctg caatatggtc atgaaccgcg tctgatggcc
     1021 gctaaatttg tatctccata ccgcatggcc ggtaaatcag ggaaaaatga tgcagctgat
     1081 gctcaggcta tctgcgaagc ggttcgtcgc ccacatatgc ggtttgttcc agttaaggac
     1141 gagagccagc aggcgatgca gtgtctgcat cgtaaccgac agggttttat cgaagagaaa
     1201 acagcaacat ataatcgtct tcgagggttg atatcagaat tcggcgtcat tgccccacaa
     1261 agtacagatg ctttgcggca catggtttct gcgcagaaga gctctttacc tcttcaggtt
     1321 cagcaatgtg tagatgattt actggaacac gttgatcgca ttgaagctaa catcactgaa
     1381 tatgaccgaa ttttgtcccg catagccaaa acagatcacc gcagtcagcg gctgatgaag
     1441 ctaaaaggag ttggtcccac aactgcctgt gctctggttg cctgtatcgg taatgcacat
     1501 gattttaaaa atggacggca actggcggcc tggctggggt tgactccatc gcaatacagt
     1561 agcggcggaa agtcgaagtt aggcaggata acaaaagccg gtgattcgta tctgcgaacg
     1621 cttctggtac agggggctcg ttcagtactg attggcgctg agaaaaggac tgacttattc
     1681 agtcgttggg tctgttcact ggtcgaacgc agaggatact ggcgcgctgt tgtggccatc
     1741 gccgccaaaa atgcaaggct gtgctgggca tcattgcatt acggtgatga tttcaggctg
     1801 tattcagcaa gctaaagcag gaagaaaccg tatgaccatc tgatctgcaa ctcgttgatg
     1861 ataaagggtt agaccccgtg aggtatatct gattattgta caggatatac gtatccgctt
     1921 aacgaacaag agcctcacgc gcgtctttca tcagggtccg aatcgatagc gattcattat
     1981 ggccgtttat agtaccgcag tctctcccct ttatttttaa ctgtagtgca gacacaaacc
     2041 ataaataccg acgttgactg ttcggggaag cccttatagt acaaaaatgt ccgatctcca
     2101 aaccgccccc acatacggca gtcggtttac gcttacagtt aagacgatca atttcttatt
     2161 attgatcgtt tatacaagtc aatatattct tttctgacat acactgcccc tgaaaaagtg
     2221 caacgtttga ccgtaaggcg cgtgcctagg gaaattcaca gactccaaga aagagaggaa
     2281 caacggatgt cagaaacggt acagattgag tcgttcaacg cagaattttt gaccaaacag
     2341 aaactacacg acctgatttt ttaccgcaaa agttttgata tcacgaatgt taacgacatc
     2401 agtgaaaccg tcaaaatagt ggagtatgtg attgagcatc agcagaagaa attaacctgc
     2461 cgcgtataca ccgaatacag aagcactgcg gtggcaggct ccctatggtc accaacagca
     2521 cttctgggag ctgtgagcgc tgccgcaatc ggtgtccata atctggcgac atggaacccg
     2581 gactatgaaa ttggtaagaa ttacgtcaaa agaaccatat ctgtaaggta taaaaagtaa
     2641 cgggtttata tctgtccggt tcagccaaaa tcctctccca taattcctat gcgagctgaa
     2701 cgggggcagt ctggagatcg aaaaattttg aacgttaagc gcattattta accaatagat
     2761 atgcataaca gattgatttt gcgtcaacgt ctatcccttt taagttatcg ccattgcagt
     2821 attgcattta ggttatccga ttgaggtcaa atcctctgaa aacgccgtat agcacgggag
     2881 tacacccgag cgcgataacg caaccgtaca atattgtacg tttcccaaac gcagcccttt
     2941 aatgtgattt aaactgtgaa atagtatggt tttcagttat ggaaacgccg ggagcgggga
     3001 aaacttgctt tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac
     3061 gatgttccgg ggactgctga tcagcccctg ttgtattcag ccattctccg gtcatctgca
     3121 gcagttttct ggcatcgtgg cttctgagca acggaatacc agcctgcctc agaacctctt
     3181 ccagaagaat atcccggcgt ctgcgttccg gtcgtaaatg actggcatca tccagctcta
     3241 ctgctgcaac aatactgaac gaacgccgct caacgataac cacatcaaca tgccactgag
     3301 acaccatcct gaataactgc caccactggc gtgatcgtgg ccggatattc ccgttcagct
     3361 ggacgatatc cgcgacccgt acctgcggac acagatacca ccgcttcata tccacaattc
     3421 tgaacagtcc tttcataaaa ctgacttccc ttcctgtcag cagcgtcttc tgacgttgcc
     3481 agcattcacc actggacagt attcgctctg catcaccggt ccgtatacct gcctcatgca
     3541 attgcatcag caaaggagaa tgctgattta cacttctgag gataatccgg ataagtaacg
     3601 ctgcaat
//
LOCUS       DAPQZD010000285         3593 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPQZD010000285 DAPQZD010000000
VERSION     DAPQZD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.291"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(123..1442)
                     /locus_tag="RO454_004876"
     CDS             complement(123..1442)
                     /locus_tag="RO454_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDY0936569.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1573..3108)
                     /gene="gadC"
                     /locus_tag="RO454_004877"
     CDS             complement(1573..3108)
                     /gene="gadC"
                     /locus_tag="RO454_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDY0936570.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3264..>3593)
                     /locus_tag="RO454_004878"
     CDS             complement(3264..>3593)
                     /locus_tag="RO454_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDY0936571.1"
                     /translation="AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
BASE COUNT          930 a          890 c          908 g          865 t
ORIGIN      
        1 ctaagtgtgg ttaattgcgt ggttaatttg acgtatttaa gcatgattgc tagtgggata
       61 gttcaagagg gtaacaagcc agtgggtaaa gcaccggctt gttacaaagt aagaatggga
      121 gtttaactgc cccaacgact ttgcagatag ctgaccgctt gttgagtctg cggtttattc
      181 agatagtcct cacggaacaa gattgtgccg ctaatttccg gcacagcatc gttaagatcg
      241 agctgctttt tcagttccgg tacgccgccg ttaatcatcc agtctggctc tatctttgac
      301 ggttctccta ctttatagaa ggcgataccg atatacaggc gggtcctggt cggtttaacg
      361 acatccgccc accattttgc caacacgtca taacgcgcgg cactccgtga gaatggccag
      421 taaatttggg gagcaatgta atccagcaac ccttgttcca cccatcgacg ggtatcagcg
      481 taggattcgt cataggctgc cgcgcctcgg gtatcggaac cgagcggatc gtgtgatcgg
      541 ttacgccaca cgcctgccgg gctaacgcca aattcgactt ccggcttaat gcttttaatg
      601 gtgtgcgaaa ccttcgcaat taactgctgg gtattgttgc gccgccagtc tgcttttgac
      661 gcaaatgcgc ctccgtattt gcggtacgtt tcgttatcat ttagccgtga acctggtgac
      721 tcagtataga aatagtcatc aaactgcacg ccatctaccg gatagcggga aaccacttcc
      781 gcaactattg atgtgatcca gtcctgaacc tcagggatgc ccgggtcgag gacaaagcga
      841 tcgccggacg ttctgatcca gtcgcggtgt tgcacataga cgctggccgg ttgttgagac
      901 agggtgctat tcagttccct gatagtaccg ggcttcgtat taaccgatac gcgatagggg
      961 ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt cgtcgagcat aaattgcagc
     1021 ggatcgtaac ccggattttc accaatctta ccggtcataa gatcggacca cggcaaaatt
     1081 ttcgatggcc acagggcggt accatccggc ttgacctgaa aaaagaccgt gtttatgccg
     1141 agacgttgca gatgatccag tttgtcgatc atcgcctgtt gttgtacacg tgcccggctg
     1201 gtggggttac tattgttaac cgaggaaacc ggtggccagt cgaggcgaga caccgtggcc
     1261 agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg tggctggtgg ctttgagccc
     1321 gctggtggtg tcaccatgga ctctggaggc gtgcttttac aactacacag taaaagtgcc
     1381 agtgcaacca gtatcgctgg tcttctaatc gctaatttct cgtttcggga gcagatatcc
     1441 atatgtgctg gtttccggta aacagatgtg cgctcattct cgtacttatc ccggttaagt
     1501 caatacgaca acaagcacga aaaagggagc gatgaattat cgctcccttg tcttataagc
     1561 attcagacat ggttagtgtt tcttgtcatt catcacaata tagtgtggtg aacgtgcacg
     1621 cgggtgcagg aagaagtgac cttttggtgc gttctgactg ttgattggct ccagagtgac
     1681 gccggtattc gctttgcttt tacgatcatg aacagcatag agaataaagg gcagggcaag
     1741 taccaccagg aaactaacga ccagtaattc aacatacata tcggtagagt caccctggat
     1801 gttatccggc ggcaggaagg aaacaataaa cgccataatt gaagtcagca gaccgacaat
     1861 agccacgacc agtttcaccc ctttaccacc agggatatta aatgtgcgtt ttaagtcagg
     1921 atgtttaagc accaacacaa tgtagccaat aaacagcatg aaataagcac acagataaat
     1981 caccaccgtc agcgccagtg cgatcaggaa ggacatgttg ttaccgccac cggtattggt
     2041 gaggatgatc aacgcgatag aagtaatcac caactgcgaa atgaccagcg ttaccggtac
     2101 gccatttttg ttcattttag cgaatgccgc tggcagcagg tttttctgcg ctgtgacata
     2161 catcccgcga gaaggaccaa caatccagga agcgatttcc gccagaacac ccagcaacag
     2221 cagtgcggag atcacgcgaa ccgtccactc aatttccggt gccacatgtg acatcagaac
     2281 ggtaaaggtt tgcattaccc ctgcggagag gttgatttca ttacccggaa tgaccatcgc
     2341 aatagacaaa ccgccaacag agcttaggca gattgccgcc accatcagca gtaacatagc
     2401 cagtggatag tcgcgcccgg ggttgctcat ttcattgacg tgggttgcgg aagcttcaac
     2461 gcccatataa ctcaaaatga aggcaacaaa tacaaccagg gtgcccactt tagagaagtc
     2521 agggaagaag gtcttcgcat ccatttcgat agcaacgggg gcaccggagt gcagataaat
     2581 agccgctaat gcgatcaaaa taaatgcagg taacaggata ccggcgaaga agccaacttt
     2641 agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt aatgccagcg cccaaagaat
     2701 gatgagtgct gcaatagttt tggtaatggg gtcttcattc agcgctggcc atttcaggat
     2761 gtaggagagt gcccctaaca cgaaatagag catcggaata aaaccaatgg cgatttgcag
     2821 atagccaaat gagatcgctg caaatcccca tctcggcccc agagtatttg atacccaggc
     2881 gaagacgcca ccttcttccc agccgtcgac ggtagccatt tccgcagcac aaagtcccac
     2941 gggaataaac cataaaatcc cgcctaatag caggaagaag actaatgaaa agcccgatgt
     3001 tgcgaaggta gggtattcat aaacagccat taccatcgat gccgttatgg caaaaaatcc
     3061 gagtaatgtg agctgcttag ctttacctgt ctgtactgat gtagccatat tatcccccta
     3121 aaacggtatt cctgtcggaa ccgcacctgt tttgttctga agcgtattca gaacaatatt
     3181 ttccgttgct aatgccagtg aacagacttt ggaaattgtc ccgaaacggc ttcgtttcgg
     3241 gacaccgtta ccgttaaacg ttatcaggtg tgtttaaagc tgttctgctg ggcaataccc
     3301 tgcagtttcg ggtggtcgct gagatatttc agggaggctt tgtagtcttc cagcaacagt
     3361 tcagcaaagt ccatttcgaa gccgcgacga cacataatgc gcatcaccac gatgtcggtg
     3421 gcttcaccgc cgagagtgaa ggccggaacc tgccagccgc gcagacgcag acgttcagag
     3481 aggtcgtaca gggtgtatcc tggatcttca ccatctttca gtttgaagca aaccgccggg
     3541 atgccttcgt ccgggcgacc ggtacagatg aactcatacg gccccagttt ggc
//
LOCUS       DAPQZD010000286         3537 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPQZD010000286 DAPQZD010000000
VERSION     DAPQZD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.292"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            67..165
                     /locus_tag="RO454_004879"
                     /pseudo
     CDS             67..165
                     /locus_tag="RO454_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
     gene            260..3523
                     /locus_tag="RO454_004880"
     CDS             260..3523
                     /locus_tag="RO454_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDY0936572.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYMNPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERNNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSSLN"
BASE COUNT         1055 a          803 c          811 g          868 t
ORIGIN      
        1 aaatttgatg aattaacaag caatttcaga gagatgatga ataactcatt aattcaaaac
       61 gaatatctca ccaacctccg aggcaccttg ctcccgaaac tcatctccgg cgagctatcc
      121 ctggaagatc ttccggatct caccaccgat acagaagccg cataacgcat tttgcccctg
      181 taaaatcagg ggctttctgg taaggttttc tactgatacg ggaatgctta ccagaaatta
      241 gccagggttg gagcgcgata tgagtctctc tttcagtgaa gcaaaattag aacaagcgat
      301 cattgaactg ttacaggatc agggatatca acacctgatc ggcgataatg tcccacgttc
      361 gagcctcgat caggtcatta tcgaagacga tctccgtcat tatttagcgg cacgctacca
      421 gcctgatggc attactgaag aagagattca gcgactgatc aaacagttca ccacgcttcc
      481 ggcctccgat ctttatgaaa gcaacaaaac attttgtcgc tggctggcaa atggttttct
      541 gttcaaacgc gacgatcggc aacaaaaaga tctctatatt gaattgctcg acacccggca
      601 tctacctgcc gcactgcgcc agatatttga cgccgaagat gtcccgttgc aacaggcggc
      661 ggaactcccg ccttcctata tgaatccgcc gcttaaccgg attaagattg ttaaccagct
      721 taaaatctcc ggcaaagata atcagactcg tattcctgac ggcattctct atatcaatgg
      781 tctgccattg gtcgtctttg aatttaaaag tgcggtgcgc gagcaggaag ccagtattgg
      841 cgatgcctgg aagcagctct gtaaacgcta tcgccgggat attccgcaac tgtttatcta
      901 caacgcgctc tgcattatta gcgatggcgt aaataaccgg atgggcaacc tgtttgcgcc
      961 ctatgaatac ttttactcat ggcgcaaagt caccggtaat gaaaaccgtg aacaggacgg
     1021 aattccatca ttgcactcaa tgattcaggg gctgtttcat ccggtacgtc tgctggatgt
     1081 gattaaaaac tttatctgct tcccggataa agccaagcac gaagtaaaaa tttgctgccg
     1141 atatccgcag tactatgccg cccgcaaact ctattacagc atcaagcaag cgcgtaaacc
     1201 tttcggtagc ggtaaaggcg gcacttactt tggcgcaacg ggctgtggta aaagttacac
     1261 catgcaattt ttaacgcgtc ttttgatgaa gagcgtggag tttgccagcc cgaccattgt
     1321 tttgatcacc gaccgcaccg atctggacga tcagctttct gcgcaaatgt gcaacgccaa
     1381 aaattacatt ggcgacgaca ccatcgttcc cgttaccagc cgtgaagatt tgcgtaatca
     1441 actggcagga cgcaatagtg gcggagtctt tctgacaacg atccataaat tcaccgaaga
     1501 caccgaactc ctttctgaac gcaacaatat catttgcatc tcggacgaag cacatcgtag
     1561 ccaggttaac ctcgaccaga aagtcatcgt tgataaagaa agcggaaaag tgcgcaaaac
     1621 gtatggcttt gcgaaatacc tgcacgattc actgccaaac gccacctatg ttggctttac
     1681 cggcacaccg attgacgcga cgctcgatgt cttcggtgag gtgatcgaca gttacaccat
     1741 gaccgaatcc gttcaggatg aaatcactgt acgcatcgtg tacgaaggcc gtgcggctaa
     1801 agtgatcctg gactccagca aactggaaga agtcgaaaag tattacgaag agtgcgcaaa
     1861 cgcaggcacc aatgagtggc aaatcgacga aagcaaaaaa gccaccgcca ccatgaatgc
     1921 ggttctgggt gatgaagatc ggttaaaagc cctcgcagaa gattttgcca aacattatga
     1981 aaaacgcgta gccgaaggtt ccaccgtaaa aggcaaagcc atgtttgttt gtgccagccg
     2041 tgaaattgcc tgggatttct accgtcagct taaagctatt cgccctgcct ggtttgaagt
     2101 gaagcaagca cccgatggcg tcgtcctgac ggaacaggag cagaaagagt taccgccttc
     2161 agaaatggtg aagatggtca tgacgcgcgg taaagatgac gacgaggcgc tttatgattt
     2221 actgggcaca aaagaatatc gcaaagagct ggataagcag ttcaaaaacg ctaaatcgaa
     2281 tttcaaaatt gccattgttg ttgatatgtg gctgaccggt ttcgacgtcc cggaactgga
     2341 taccatctat attgataaac ccttacaaaa acataatctt atccagacta tttctcgcgt
     2401 taaccgtaaa ctggaaggca aaagtaaagg gttagtggtg gactacatcg gcattaaaag
     2461 tcagatgaac caggcactgg caatgtattc ccgcattgat gccaccaact ttgaagatat
     2521 tcagcaatcg gtaatagaag ttaaaaacca tctcgatttg ttggggcaag tcttttacga
     2581 atttgacagt cgggattatt ttagtggtga gccacaagcg caattatcct gcctcaaccg
     2641 cgcggcggaa tttgttctgc gtacccagaa agttgaacgt cgttttatgg gactggttaa
     2701 acgcatgaaa gccgcctacg acgtctgctg cggcagcgaa gcattatcac agacagaacg
     2761 tgactatatt cactattatc ttgctgttcg ttcaattgtt ttcaaactga cgaaaggtga
     2821 cgcaccggat gttacccaga tgaatgcacg cgttcgtgaa atgattgcag aagcgttaaa
     2881 agctgatggc gtagaagaaa tttattttct tggcgataaa aaagcggaat ccatcgatat
     2941 ttttgacgaa gattatctgg cgcgaattaa caagatcaaa cttccggcaa cgaagatcca
     3001 gttattacaa aaattactgg aaaaagcgat cagcgacttc aagaaagtga accagttgca
     3061 agggattaac ttcacccgcc gcttccaggc gattatagat cgttataatg agcggcgaga
     3121 agatgatgta ctcaacggtg aagaattcga tacattcagt caggaaatga ccgatattat
     3181 ctatgatatt aaaaccgaaa tgggcacctg ggccgattta ggcattgata ttgaagaaaa
     3241 agcctttttc gacattcttg ctcatatgcg cgataaatat cagttcacct atgacgatga
     3301 aaaaatgctg tcactggcaa aagagatgaa aagcgtggtt gacaacacat ctaaatatcc
     3361 tgactggagt aaacgcgatg atattaaagc gaaactgaaa gttgaactta ttctgcttct
     3421 acacaagcat aagttcccgc cagtagcgaa tgatgatgtt tatatgggtg tgctggcaca
     3481 ggcagagaat tttaaagaac accatatgag ttctttaaat taattatgaa ggccgga
//
LOCUS       DAPQZD010000287         3523 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPQZD010000287 DAPQZD010000000
VERSION     DAPQZD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.293"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..355)
                     /locus_tag="RO454_004881"
     CDS             complement(<1..355)
                     /locus_tag="RO454_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936573.1"
                     /translation="MFIEAFASLMQKAKIGTVGTDIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIP"
     gene            complement(357..830)
                     /locus_tag="RO454_004882"
     CDS             complement(357..830)
                     /locus_tag="RO454_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936574.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKADFTRG"
     gene            complement(821..1165)
                     /locus_tag="RO454_004883"
     CDS             complement(821..1165)
                     /locus_tag="RO454_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936575.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            complement(1245..2078)
                     /locus_tag="RO454_004884"
     CDS             complement(1245..2078)
                     /locus_tag="RO454_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936576.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            complement(2078..2512)
                     /locus_tag="RO454_004885"
     CDS             complement(2078..2512)
                     /locus_tag="RO454_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936577.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIDGILKAQGGE"
     gene            complement(2557..3477)
                     /locus_tag="RO454_004886"
     CDS             complement(2557..3477)
                     /locus_tag="RO454_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDY0936578.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPFSQSVNITPASPSVLVGKTVQLSAGITMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESKNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTVAQE"
BASE COUNT          860 a          948 c          747 g          968 t
ORIGIN      
        1 caggaatgcc tgcttctatt aatgctccat catttttggg ataaatgatt ggcatcgcca
       61 tcggtcgtac cagtcgaaaa taaacaccac cagattccgt ttcctctact ggaaacatct
      121 caatgatttt attggctttc tcaaccgtcg tagtaattga cgaaccacgc acaattattg
      181 tgaatgattc gtggtaaaag ccctgtagct catgatcgat gctgataccg gtatttgggt
      241 taataagcag gacgccagat ttcacattgg caggcaagta gtgacagaaa atgtcagtcc
      301 cgaccgtgcc aatctttgcc ttctgcatca aacttgcaaa cgcttcgata aacacattaa
      361 cctctcgtaa aatcggcttt tctggcagcc tcaagaatcg cttctgagaa ctgcttctcg
      421 ctaatttcca ccgctctttc cagaaagtgt ggcccaacac gaggtttaac accggcaatt
      481 ggtgggtttg tcacgttctt cattcgagaa agataaccga gtcggtattt acccagctcc
      541 atgtacttag catagtcacc gacttctacg cccggatgcc cctgacgtgg ttttgctcca
      601 gacacagaaa gctcaatgcg cagccctgaa taaccttctt taatcaccct ggcaaagatg
      661 gctgtctcca gagatccggt ttccagcggg gccattgcac ggctgagacg ctcaaccaaa
      721 cgcgccagct tttccatgtc ccgaataaga tatcgcttaa atgctttctg gctgttattg
      781 agtctattcc ccgcacgttt gaactgatgc gcatcgtatt tcagacccat atattcgccc
      841 ccacttcaag atgcccaggt cttcctcgta gcccccaacg gcgatgaaca ctggacacct
      901 ttaatttttg accttcaagg atcagcacat catcaagttg tacagccgct tctaaaggga
      961 caactaacac agcatcaaac agctccagac tcgccttacc acgactacca gagctatcag
     1021 cacgaactga cgatttctca ttactctgtt cgaacttaac cacgccgaca ttcgtcttcc
     1081 tgacgaattg taattgcgcc tcgccgtaaa cgttctttgc gccaaagcgg tagatcgcaa
     1141 tttctgtttg ccacgaaata ttcatgctct ctctctgttg ttgtcggtcg ctctcattac
     1201 tggccaaaaa ccctttcgac caaaagtaaa taatgcgact ggcgttacgc acggcgaaca
     1261 atcatacggt tattgatgta actgaccagc aagcgccagg tactgcgagc cacatgcacg
     1321 tttgcagctt taccggtacg gtacatgttg gttgtttcac cgattgactc tgacaaaatg
     1381 ccatcctctc gtgctgcggc aacatcattg ccatttgcga tctcacacgc ttcgttgaca
     1441 acggcaagca tcaacgcttc tttgaagtag tcagggaact cttcaaactt ctcctgcgtc
     1501 atcttttccc aatcgactaa atcatgccgg tacgctccat ctgctcccca cggaatgtca
     1561 tacacattca gcatattttg agggcgatcg tatcggtcaa agtcgatacg tagaattttg
     1621 cggattgaga acggtaaagt tttaattcgt ctggtagcct caatgagacg cttgcgcatt
     1681 aagccttcac catccgacag caaagtgtcc ccattcagca tatcgatcgc ctgcatttga
     1741 gcatcagcga cagttgcaaa cgactgttct ggtatcgaca gttcaaaact attcagcaga
     1801 acatacattt gccgctcttc atgcgtcaga cccgatgcaa cagccttcac aatgacgtac
     1861 cgcagatctc gctctttctc agagagctgg ttatattcag ccgacacgac aaccggaatc
     1921 gacatttgac cgctggtaat ttctagcggc tcgccatcaa cgagaatagc cccggtgctg
     1981 tcctttactg tgtaggtagc agattcgata tccagcacgt tgaaggcaaa tgaaagagaa
     2041 acagcttcac cgctacgata cgagtcgatc tgcgccatta ctcaccgcct tgtgctttca
     2101 ggatgccgtc aatcatttcg acaattcctt tcgctttaac accaacctga ttaccaataa
     2161 ctcgtagacc ggcaatacct tcgttgtctg caatggactc cagctcttct cgtgtgaaag
     2221 tctgaatctg tttggccggt tcatctggtg tcccacgttt cattggcaca atgtcaggcg
     2281 ctgctggctc ggtaatcagg tctgcggtca attcaccacg atcactgtat gcggcagacg
     2341 gagaaacatt tttgccctct actgttgacg ctcgcattga agcacaaatc ctctgctgat
     2401 cgataaaagg caactctgcg acggacaccc cattctcgaa ctgaacgccg cacaacattc
     2461 ccgaataacc ggaaaattgc ggttctaata aaacaatttt tgctggtttc ataacgcttt
     2521 ctctctacat gggcggcgaa agccgcccaa tatcggttat tcctgtgcga cagtaacttc
     2581 tacagtcgct gtcgctttgt ggctaccatc ttgggtagta acctcgattg tggcagtgcc
     2641 agccgcaaca ccagttacaa caccggtttc gctgtccacc gttgcaaact cggtattttt
     2701 ggattcccaa gtaacggttt tatttgttgc tcctgccggc tcgacatttg cggtcagctg
     2761 aacagttttg tttgcctcaa cggttgtgct atttgggtcg atttcgacac cagtaacagc
     2821 cacaacaggc gcagttactt ccaccgttgc agttgccttt ttgctgccat cttgggaaac
     2881 gatctcaatc gtcgcagtgc caacttctac accagtaacg ttcccgctct ggtctaccgt
     2941 tgccttgtct tcatttttgg aagaccaggt aactgcctta ttcgtcgcat ttgacggctg
     3001 aacattcgct ttcagactga ctgatttccc tttctcaaca gaggttgagt ctggcgttac
     3061 cgttacggat tgaacggcta ccggatttac ggtgacttcc actgacgcgg aaagctgggt
     3121 ttcctgatca gttgcggtaa ttttcacttt gcctggtgct acgccagtta ctaatccagt
     3181 gccgttgacg gttgcaattt gatcattggc tgacttccag gtgaatgaac tggcactctt
     3241 acccatagta atacctgcac taagttgaac agttttccca accaaaactg acggtgaagc
     3301 cggggttatg tttacggatt gggagaaggg aaccgcctgc aaacaagcag atagctgact
     3361 ttgttgtcgc tcggttaaag gttcatcgga gatagaattg gtaaatccgg cacggcacat
     3421 atgccccgta aaatccgaaa atgcctcttc cgtaatcttc atcttttgtt ctggcatttc
     3481 tcgctcctac aaaaagggtg ggcgtatagc ccacccttta aca
//
LOCUS       DAPQZD010000288         3459 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPQZD010000288 DAPQZD010000000
VERSION     DAPQZD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.294"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            70..501
                     /gene="osmC"
                     /locus_tag="RO454_004887"
     CDS             70..501
                     /gene="osmC"
                     /locus_tag="RO454_004887"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDY0936579.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(691..3090)
                     /gene="dosP"
                     /locus_tag="RO454_004888"
                     /gene_synonym="pdeO"
     CDS             complement(691..3090)
                     /gene="dosP"
                     /locus_tag="RO454_004888"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDY0936580.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3115..3278)
                     /locus_tag="RO454_004889"
                     /pseudo
     CDS             complement(3115..3278)
                     /locus_tag="RO454_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT          880 a          848 c          852 g          879 t
ORIGIN      
        1 atcctgctta tcctcgtgct gtttctcacg tagtctataa tttcctttta agcccacagg
       61 agagcaacaa tgacaatcca taagaaaggt caggcacatt gggaaggcga tatcaaacgc
      121 gggaagggaa cagtatctac tgagagcggc gtgctgaacc aacagccgta tggatttaac
      181 acgcgttttg aaggcgaaaa aggaaccaac cctgaagaac tgattggcgc agcgcatgcc
      241 gcatgtttct caatggcgct ttcattaatg ctgggggaag cgggattcac gccaacatcg
      301 attgatacca ccgccgatgt ctcgctggat aaagtggatg ccggttttgc gattactaaa
      361 atcgcactga agagcgaagt tgcggtgccg ggtattgatg cctccacctt tgacggcatt
      421 atccagaaag caaaagcagg atgcccggtt tctcaggtac tgaaagcgga aattacgctg
      481 gattaccagt tgaaatcgta aagcgttgcc ggatgacgcg tcaggcgcgt gaatgcctga
      541 tgcgtcagca caatcacatc aggctgagaa gggattgttc tgacatggct atgcgttgtc
      601 gttgtccaac taaacgtagc ggaaatcaca gcatttaagt gacagtgtca cgttaaataa
      661 aaacccgcga gtgcgggcga gaggaattta tcagattttc agcggtaaca cgctgctcat
      721 ccagcctgga atttcttcgg cgggcagggg gcgggaaaag aaatatccct gaataacgcg
      781 acagtggatc ttgcgtagca tctcaaattg ctctttggtt tcgacgcctt ccgccacgac
      841 ggttaaattg aggctttgcc caatgctggt aatggcttcg agtaaggcaa ggatgcgttt
      901 ttcagtcaga caacgatcga caaaactttt gtcaattttg atttccgtta ccggaagact
      961 gactaagcgg gataatccgg aaaagcccgt accaaaatca tctaccgata aaccgatgcc
     1021 catatcgcgc aggatctgaa tgcgcttaaa gatttcggta tcgtgttcca tcatcatgct
     1081 ttccgtgatt tccaccgtca gctggtggcc gtcaataccc caggcgtgca ttgcatcaga
     1141 tacctgatta ggcagctgat tactgcgaaa gtgcagcgca gacaagttaa cggataacgc
     1201 cgggatatga atattctggc tacgccattc tgctaactga cggcaagctt ccgcgatgac
     1261 ccagcgccca atattttcga tttcaccaat ctcttctgcg agaggaataa accgtgaagg
     1321 gggcacatga ccatgcaggg gatcgtacca gcgagcaagg gcttcgatgc cgtacagttc
     1381 acccgtttct gcgaagattt gcggctgata aaccagttta agttggttat tgctaatcgc
     1441 ttcttttagc gctgcgccta aaaccaaacg ctcttttacc atttcgttca tcgccgggct
     1501 gaagaactgc cagccgttac cgccattttt gcgaataaaa tccattgcat tgtgagcagt
     1561 ggagagcagg taatcgcggt ttttacccac gtcgtagctg atgccaatgc tcaaggtgag
     1621 cgggaatggt ttatcgtcaa tcattatcgg cttgctgacc acattccgta gctcatcggc
     1681 gatctgggta atgttactga catcgttttc caggctcacg aggacaaaag acgcaccttc
     1741 aatacgacag agatactgat ccggtttgag tttttcacga aagcgattga ccacttccag
     1801 caatgcctga tcggcccacg catagccaag gctatcaatc acatcctgaa tatggtcaac
     1861 accgatgaga tacaccaccg gagagacggc tttgtcgacc aggtcatcga ggtaattgtg
     1921 caggttattg cgatttggca gaccggtcat cggatcaaat tggatgagtt gttcaatatg
     1981 ctgacggctt ttttcctgtt ccagcgccag cgcggccata tgctggctga tatctgccac
     2041 gcgttcgata aaggcgctgg tttctgctcc tgacgaggtt ttaatttgca ggatccccgc
     2101 aggcgcgcca tcgcgctgac gaatggtcgc tgaccagctt tgcgcatttt gaacttctgc
     2161 accgtgcgaa gatgacgccc agtgtatcgg catcccgttg cgcagcgcga acagagaaac
     2221 atgcgattcg ttgagaacag attcgatatt acgacaaatg atttccccca tttcatgaaa
     2281 tggtgggctg ctgcacatgg cggcgagaat attgccttca agctggcgga tttgcctttc
     2341 ttcggtgata tccgagaatg tcattaccag gttctgcaga tgcgcgagca cgtcataaac
     2401 cgggctgata gaggctttaa tccagatttt ttcaccggtg cgcgtcaaca gcagaaattc
     2461 gtcctgatcg cgggcggttt tccatagcaa ctgctgtaaa cgaatacggt tatcagcagg
     2521 gaattctgga atgttcagga gtgtatcggg ctgcataccg ctggcttcgt taatgcagta
     2581 accaaacatt tcggtaaatg cgcgattgca ctgcacaata tggcgttccg gatcgaggac
     2641 aatcaccggt cggtcgagat ggtcaacggc aataatcaat tgtcgggtct gttctttttg
     2701 cgccatttct acgctggcat cccgtaccag cgccaggtaa taaactttcc cctcggcgct
     2761 cactttcgat agcgcaaaac gggtccagat tttactgccg tcttttttct ccagctgcag
     2821 ctcccgactc atcccctcga cgcgcgcttt accaccttca cggttgtgac gaatgtattg
     2881 aggatgcgca ggacgcaaat cccgcggaat cagcatatca atgttattgc caatgacttc
     2941 ttcacgtttg tacccccaga gcttctctgc ggcggggttg aaaaacatca cttcatcatt
     3001 ttcgttaatt aacaccgcgc ccatcatatt ttgctcaagg gcggggaaaa aaatgccatc
     3061 ggcggcatta tccgcatcgg ttagcttcat gattacctct gcatcctggc gcatctaaag
     3121 actggcttta cagagttcaa cacggtttct acctcgtctt ttgcgatata cagagcttca
     3181 tcggctattt gaatgaggcg ctcatagtca ggatgaccat taaacatggc ggcaccgatg
     3241 gaaagtgaga gggcaatatc ctcgccgttt gcggctttaa aataatgtcg acctttatgg
     3301 ttaaaccgca ggccaaaatc aaccaaacac aacgtgcggg attggctaac tgcaccatat
     3361 tctcctgaaa tatgaagata tactgaaaag aaataagcga cttaggacag tttcaatcta
     3421 cgctactgtt cttcagaaga gtatagccca tcgtaatta
//
LOCUS       DAPQZD010000289         3364 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPQZD010000289 DAPQZD010000000
VERSION     DAPQZD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.295"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="RO454_004890"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="RO454_004890"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(210..737)
                     /gene="mobB"
                     /locus_tag="RO454_004891"
     CDS             complement(210..737)
                     /gene="mobB"
                     /locus_tag="RO454_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDY0936581.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(719..1303)
                     /gene="mobA"
                     /locus_tag="RO454_004892"
     CDS             complement(719..1303)
                     /gene="mobA"
                     /locus_tag="RO454_004892"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDY0936582.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1373..1642
                     /locus_tag="RO454_004893"
     CDS             1373..1642
                     /locus_tag="RO454_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDY0936583.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1719..2704
                     /gene="srkA"
                     /locus_tag="RO454_004894"
                     /pseudo
     CDS             1719..2704
                     /gene="srkA"
                     /locus_tag="RO454_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
     gene            2721..3347
                     /gene="dsbA"
                     /locus_tag="RO454_004895"
     CDS             2721..3347
                     /gene="dsbA"
                     /locus_tag="RO454_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDY0936584.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          851 a          818 c          831 g          864 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcgaa
      121 aggcccagtc ggaagactgg gcctttttgc atatgtgcgg aataaatgcc ggatgcgacg
      181 ctggcgcgtt tgcttcagcc tattcttgct tatccgtcct gcttttgcat ccactccacc
      241 acaaaatcag ccagcccctc aacatcatta atatccagta acgcgacatc aagattaagc
      301 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc cggtcgatgt
      361 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      421 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaaatgtag atccagctct
      481 tcttcgtctg gtgtttccgt catcaaggcc catcgctgct ggctggcaac gatggtttgc
      541 gccgcgccag ccttgcgcag ctcatagcta tctttgcccg gtttatcaac atccatatca
      601 tggtgcgtat gcttaatcag ccccggacgg atccctctgg cgcataatgc cgggatcaat
      661 tttttcagca gcgttgtttt tccggtgcca ctccatgcag caaaggcgag taacggtatc
      721 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgtttata aatgcctctt
      781 tacgatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc ataacccggc
      841 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      901 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      961 gagtaagccg ggcggctaaa tcatggggaa tgtaaggcgt atcgcacggg caaaacaaaa
     1021 accactcacc cgcttcctgc tgcattactg aaagcattcc tgccagaggg ccagggtaat
     1081 ccgccagtga atcttcaatc actttcagac cgcttacctg atagatttcc tgatgacgat
     1141 tagcattaac cacgacgtga gaaagctgcg tcataagcgc gtcagcgaca tgttgccata
     1201 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1261 cgcccgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1321 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1381 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1441 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1501 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1561 ggtgatcccg ggtttgcaga aagattatga agaagatttc aaaacggcgc tattacgcgc
     1621 acgtggcgta attaaagagt aaaagcttgt aagcggcgca accaaaatca tcgtgaaatg
     1681 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1741 tccagacact acacccggat accatcatgg acgctctgtt tgagcatggg atccgggtgg
     1801 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1861 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1921 tcgaagaaca tcaatttgcg ttgcagctgg ttaacgatga agttccggtc gcagcacctg
     1981 tggcctttaa tggtcagact ttattgaacc atcagggatt ttatttcgct gtttttccaa
     2041 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     2101 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2161 gtttgaatga atatctcatt gagccacgca agctgtttga ggacgctaca ctgatacctt
     2221 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gattgccgcc gttacagcac
     2281 actggcggga agatttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2341 tctggcgcga tggcccaatg tttgttgatc tggatgatgc acgtaatggt ccagccgttc
     2401 aggatttgtg gatgttgctc aatggcgata aagccgagca gcggatgcaa ctggaaacta
     2461 ttattgaggc ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2521 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2581 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggcta cgacagacgg
     2641 cgacttttat agaacaggca aaagttctac aagaacccct ttgcaattaa cacctatgta
     2701 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2761 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2821 cagttgctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2881 agtttgaaga agttctgcat atttctgata acgtgaagaa aaaactgccg gaaggcgtga
     2941 agatgactaa ataccacgtc aacttcatgg ggggtgacct gggcaaagag ctgactcagg
     3001 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cacagttccg ctgtttgaag
     3061 gcgtacaaaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3121 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3181 tcgctcagca ggaaaaagcc gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3241 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagtaatatg gatgtttttg
     3301 ttcagcagta tgctgatact gtgaaatatc tgtccgagaa aaaataattc aatgtaaata
     3361 acat
//
LOCUS       DAPQZD010000290         3313 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPQZD010000290 DAPQZD010000000
VERSION     DAPQZD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.296"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            328..1644
                     /gene="shiA"
                     /locus_tag="RO454_004896"
     CDS             328..1644
                     /gene="shiA"
                     /locus_tag="RO454_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDY0936585.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1746..3200
                     /gene="amn"
                     /locus_tag="RO454_004897"
     CDS             1746..3200
                     /gene="amn"
                     /locus_tag="RO454_004897"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDY0936586.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          766 a          806 c          802 g          939 t
ORIGIN      
        1 atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc agtctatcag
       61 aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc tcgaacctgt
      121 gggccctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat cttccatcga
      181 atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc tgtcgaggta
      241 gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc atactaatta
      301 taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac tcgtcccgat
      361 gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagttt cgctggtgcc
      421 gtcgtcgact ggtatgattt tctactctat ggcattaccg ccgcactggt gtttaatcgc
      481 gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc cacctttggt
      541 gtcggcttcc ttttccgacc gctcggcggt gtcattttcg gtcactttgg cgaccgactg
      601 ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac agccttaatt
      661 ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttgct ggtgacactg
      721 cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcagcgtt gctttccgtt
      781 gaaagtgcgc cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt tggctacggt
      841 gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac gactgacgaa
      901 cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact ggtactggga
      961 gcattgtggg tgcgcaatgg catggaggag tccgcggaat ttgaacaaca gcaacataat
     1021 caagcggccg cgaaaaaacg catcccggtt atcgaagctc tgttacgaca tcccggtgct
     1081 ttcctgaaga ttattgcact acgactgtgc gagttgctga cgatgtacat tgttactgcc
     1141 tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt ccttaatatt
     1201 ggtttgctgg taggtggatt aagctgcctg acaattccct gttttgcctg gcttgccgat
     1261 cgttttggtc ggcgcagggt ttatattaca ggcgcgttga tcggaacgtt gagcgcattt
     1321 cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg
     1381 ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg
     1441 tttggtgcca gttatcgcta cagtggtgct ggagtcggtt atcaggttgc cagtgtggtt
     1501 ggcggtggat ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg
     1561 catagcgtcg ccatttattt gctggctgga tgcctgattt ctgcaatgac cgctttattg
     1621 atgaaagaca atcaacgctc gtgatagcct ggcgaagatc atccgatctt cgccttatac
     1681 ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga
     1741 cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg
     1801 cgctgtatga gcaatctgta gttgctttac gcaacgccat tggcaaatat attacaagtg
     1861 gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg
     1921 taacctggga cggtagcaca actaatcccc ccaaaacgcg cgcatttggt cgttttaccc
     1981 acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg tatctcaatg
     2041 aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa ccctcgcagc
     2101 atgaaatccc ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga
     2161 gcgctgggtt aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg
     2221 cagacggcat ttatcatcca acggaattct ccccgctatc gcattttgat gctcgccggg
     2281 ttgatttttc cctcgcacgg ttgcgccatt ataccggtac gccagtagaa cattttcaac
     2341 cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca
     2401 gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga
     2461 tcactgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc
     2521 agatgccagc atggcattta attaccgctg atggtcaggg tattactctg gtgaatattg
     2581 gcgtgggacc gtccaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg
     2641 tctggttgat gattggtcac tgtggcggat tacgtgaaag ccaggccatt ggagattatg
     2701 tccttgcaca cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata
     2761 ttcctattcc gagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca
     2821 gcggcaggcc tggtgaagaa gtcaaacagc ggttacgtac tggcacggtg gtaaccacag
     2881 atgacaggaa ctgggagtta cgttactcag cttctgcact tcgttttaac ttaagccggg
     2941 ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc
     3001 catacgggac actactgtgt gtttcagata aaccgttaca tggtgagatt aagcttcccg
     3061 gccaggctaa ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg
     3121 cgatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta
     3181 atgagccgcc gttccgataa taagttatga accttcttat cagaaataga tgtgaaggat
     3241 taagtaagac atctggcaaa tagcctgcaa taaacgcggg ctgtttgctt ccttgggcag
     3301 atgcgagttt tat
//
LOCUS       DAPQZD010000291         3309 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPQZD010000291 DAPQZD010000000
VERSION     DAPQZD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.297"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            494..1063
                     /locus_tag="RO454_004898"
     CDS             494..1063
                     /locus_tag="RO454_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDY0936587.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1323..1724
                     /locus_tag="RO454_004899"
     CDS             1323..1724
                     /locus_tag="RO454_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936588.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            1712..2146
                     /locus_tag="RO454_004900"
     CDS             1712..2146
                     /locus_tag="RO454_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDY0936589.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            2501..2881
                     /locus_tag="RO454_004901"
     CDS             2501..2881
                     /locus_tag="RO454_004901"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDY0936590.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            2878..3225
                     /gene="tnpB"
                     /locus_tag="RO454_004902"
     CDS             2878..3225
                     /gene="tnpB"
                     /locus_tag="RO454_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDY0936591.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          787 a          878 c          894 g          750 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcccc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacaaactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatagctcc
      301 ctcgccacgc cttatccgta accggttttt tacattaaaa aaaacaacgc cgggaattca
      361 atcgtcagtc cggagacgac cgttcgggtt atcacagagt gcctgagaca gtgtcctgcc
      421 ggaggtcact tcagggactc tgcgtatttt ttacgacgtg gctattccgt atgaaccata
      481 cggagattta accatgacct acaaatacaa ccccttctgg cagcaacgta ttcgtgagac
      541 ggtgcggcac gcactgaatg ttcatccccg cctgacggca ttgcgggttg acctgcgttt
      601 cccggatgta ccggcagcaa cggacgcagc tgtgatatcc cgcttcatca atgccctgaa
      661 agcccgaatc gacgcttacc agaaacgtaa gcatcgggaa ggtaaacgcg tgcatcccac
      721 aaccctgcat tacgtctggg cccgggagtt tggggagtgc aaaggtaaaa aacactatca
      781 cctgatgctg ctggttaacc gggatacctg gtgtcgtgcc ggtgattacc gtgctccggg
      841 atcactggcc gggatgatta aacaggcgtg gtgcagtgcc ctgggagtgg atgtcgggtg
      901 ccatgccacg ctggtgcatt ttccggcctg gccggcggtg tggctggaac gcgatgatga
      961 cactggcttt cagcaggtgc tggaacgtgc tgactatctg gcgaaggaac ataccaaagc
     1021 tcactgcacc ggtgagcgca attttggctg tagccggagt tgagcacgac tggcgcgcca
     1081 atacccggca aaccaaaagt atgggttgtt gtctggcggc aacgatgtat ccactcgccc
     1141 actgactgtt ttttacgtca gtgttccatt gtgtgacaac cacagtgctg taacagcaac
     1201 cagcctgctt tacccaaact ccagtccaga ttttaactga accgccattc acaccctgac
     1261 cacgctgccc gtaccgggac ggctgttgcc tgcgtgtcgt ctggcggctg aggaatatga
     1321 ctatgtacgc aaaatccttt atcgctcttg atggcaacgg acgtctgacg ggcgcccgta
     1381 ctgcacaggc cgcaccttat gctcactaca cctgccactt gtgtggcagt gcactcagat
     1441 accatccgca atacgacact gaacttccct ggtttgaaca cactgacgac aggctgacag
     1501 agcacggtca acagtgccct tatgtcaggc cggagcgcag agaaatacag ttgattaaac
     1561 gtctgcagca attcgtaccg gatgccttac ccgtggtgcg taaagccagc tggcactgca
     1621 gacaatgtca ccacgattat tatggggagc agtactgcac acactgccag accggaggat
     1681 tcagtattcc ccggacaact caggaggaaa tatgcgaatt ctgaactgtt atatggcgaa
     1741 tgacagcaaa ggccattttg ttacggcgaa agaagctgcg aagcacaacc gacaggacgt
     1801 tttgtgctgt gtgtcctgtg gatgcccgtt aacacttcag cggggcaatg acggacaacc
     1861 accgtggttt gaacatgacc agatgactgt cgctgaaaaa atcctgctgc gatgtacctg
     1921 gcttgacccg gcagagaaag aggcccgtcg tttgcatctg cagggcatga cggttccgga
     1981 ttatacggtg aaggtgagaa agtggttttg tgtgatgtgt gacgaagatt atgaggggga
     2041 aaagtgctgc ccacgctgcg gtaccggggt atacagcagg gcgtgggggc ggcaggaggt
     2101 gccgtcggaa gatgccaggg ctgataatcc gttacagagg ctgtaatggt tgcctccgga
     2161 gcagtttgcg gtgatgcctt tcccgggaat ggattgccag tgcggggact gtggagctta
     2221 attccggtgt tggtggcctt tccattgatt ttatgccaac agccccctgt gactgacagg
     2281 ctgcctcgtc attccattcg tatctccagt aacagggggt tgtattgtat tttattgtac
     2341 cggttcaggt gtgcgtttcc cggcgcgtct gcaccggctt aaccaaattc aacagggata
     2401 aaatagtggt aagcgtacag cctgaaccgt ctggtcagaa tctgacgaat tagacaaagt
     2461 ggtgtccacc aaataagtag tgggaaccaa agtgtcagat atgcagaaaa atgtgactcc
     2521 cggcaggcga aaaggctgcc ctaattatcc tcccgaattt aaacagctgc tcgttgctgc
     2581 ctcctgtgaa cccgggatat ccatctcaaa acttgctctt gaaaatggca ttaacgccaa
     2641 tctgttgttc aaatggcgac aacaatggcg cgagggaaag ctgctattac cttcttcaga
     2701 gagcccccag ctacttcctg tgactctcga tgcagctgcc gaacagccag aatcgctcgc
     2761 agaggacccg gaaaccctca gtatcagctg tgaggtaacg ttccggcacg ggacgctccg
     2821 cttcaatggc aatgtcagcg aaaagctcct gactctgctg atacaggaac tgaagcgatg
     2881 atcccgttac cttccgggac caaaatttgg ctggttgccg gtatcaccga tatgagaaat
     2941 ggcttcaacg gcctggctgc gaaagtacaa acggcgctga aagacgatcc catgtccggc
     3001 catgttttca ttttccgggg ccgcagcggc agtcaggtta aactgctgtg gtccaccggt
     3061 gacggactgt gcctcctgac caaacggctg gagcgtgggc gcttcgcctg gccgtcagcc
     3121 cgtgatggca aagtgttcct tacgcaggcg cagctggcga tgctgctgga aggtatcgac
     3181 tggcgacagc ccaagcggct gctgacctcc ctgaccatgc tgtaaatctc tttatcctgg
     3241 ttgtcacaga ataagcccgg taaaatacgg gcttatgaac gacatctctt ctgacgacat
     3301 cttcctgct
//
LOCUS       DAPQZD010000292         3212 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPQZD010000292 DAPQZD010000000
VERSION     DAPQZD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.298"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..283)
                     /gene="ybgE"
                     /locus_tag="RO454_004903"
     CDS             complement(<1..283)
                     /gene="ybgE"
                     /locus_tag="RO454_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDY0936592.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGFFCPLLADIVLIVGLIF"
     gene            complement(283..396)
                     /gene="cydX"
                     /locus_tag="RO454_004904"
     CDS             complement(283..396)
                     /gene="cydX"
                     /locus_tag="RO454_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDY0936593.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(411..1550)
                     /gene="cydB"
                     /locus_tag="RO454_004905"
     CDS             complement(411..1550)
                     /gene="cydB"
                     /locus_tag="RO454_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDY0936594.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1566..3134)
                     /gene="cydA"
                     /locus_tag="RO454_004906"
     CDS             complement(1566..3134)
                     /gene="cydA"
                     /locus_tag="RO454_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDY0936595.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          891 a          890 c          805 g          626 t
ORIGIN      
        1 agaaaatcag cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa aacccttgcc
       61 agagaacctt ctgcggacga aagcccacgc cgtgaatcac accagcacag acggcccaca
      121 taagcagtaa accatgccag atttccagtt cactggtctt cgcggcgaaa cgtgatgggt
      181 cccaaaacat acatcctgct aacagaagtg ccatcacgaa ggaaagcgcc cgcagggggc
      241 gcttgtccat taccgcatac aaggttgcga taatcttact catcagatgt cttcttgacc
      301 ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg attaccccaa acgaacaggc
      361 aagaagcgtt cccagaatcc atgcgaaata ccacattttt agctccttac ttagtacaga
      421 gagtgggtgt tacgttcaat atcttctttg gtgatacgac cgaacatttt ccagtaacac
      481 caggcggtgt agagcagaat gatcggtacc agaaccaccg caacccaggt catgacgtta
      541 agcgtcagct ggctggaagt tgcatcccac attgtcagac ttgcgttcat catggtgctg
      601 gacggcatca caaacgggaa cattgcgata ccggcagtca ggatgatgca ggccagcgtc
      661 agggaggaga aaacaaacgc ccacgcggct ttatccatac gtgcagtcag gatggtcagc
      721 agcggcagaa ccacacccag tgccggaata gcccacagaa ttggcgtgtt gttgaagtta
      781 accagccatg cgccagcttc acgaaccact tctttattca gcgggttaga ggctgcgtaa
      841 tggtccattg tcgatttcac gacataacca tcgataccgt acatcaccca tacgccagcc
      901 agtgcgaagc agaccagcgt caccagtgcc gccacctgag ccgttgcacg ggtacgcagg
      961 tgcagttcgc ccacggtacg catttgcaga taggttgcgc cctgagtaat gatcatccct
     1021 acgctcacca cacctgccag caggccgaac gggttaagca gctggaagaa gttaccggtg
     1081 tagtacagac gcagatattc atcaacgttg aatggtacgc cctgcaacag gttaccgaac
     1141 gctacaccaa tcaccagcgg cggaacgaag ctaccaatga agatgcccca gtcccacatg
     1201 ttacgccagc gggtttcttc aatcttggag cggtagtcaa aaccgaccgg acggaagaac
     1261 aaagacgcca gtacgaggat catcgccaca tagaagccgg agaacgcagc ggcatagacc
     1321 atcggccagg cagcaaacag tgcaccgcct gcggtgatca gccaaacctg gttaccgtcc
     1381 cagtgtggtg cgatggagtt aatcataatt cgacgctcgg tgtcgttacg accgaggaaa
     1441 cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga
     1501 acgccaacca gcagccacca gataaaacgc aatacttcat aatcgatcat ttgacgactc
     1561 ctgtcttagc gtgccggctg agtagtcgtg gaagactgct caaagtgata gcgaccggtt
     1621 ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta agaacaattc tgccaccagg
     1681 aacagggtat acaggccgca aatcagcacc attgagaaga tgagatcgcc tgcggtcagt
     1741 gacgagttcg ccacagctgt cggcagcact tcaccgatag cccacggttg gcggccatat
     1801 tcagccacga accagcccgc ttctacagca atccacggca gcggaatacc gtacagcgcg
     1861 gcgcgcagca gccatttttt ctcgccaatg cggttgcgga tgacactcca gaaagagagc
     1921 gcaatgattg ccagcagcag gaagccacac gccaccatga tacggaacgc gaagtacagc
     1981 ggcgctacac gcggaatgga gtctttggtt gcctgttgaa tctgagcttc agtcgcatca
     2041 gccacgtttg gcgtatagcg tttcagcagc agaccgtaac cgagatcttt cttcatgcta
     2101 ttgaactggt cacgaacggc ctggtcagta gaaccagaac gcagttgttc gagcagagag
     2161 tacgccttca tcccgttacg aatgcgttct tcatgctgca ccatcagttc tttcagaccg
     2221 ataactggag tatccacgga acgcgttgca atgatgccca gcgcgtaagg gatctggatc
     2281 gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga acagagtaaa ggcagcaggt
     2341 gcaggttgcg tttcccactc ggcttcaata gcagccagtt tggttttctg cacgtcgccc
     2401 atttcgtagc cggattcatc acccagaaca ataacagaca gaacggcagc cataccgaag
     2461 ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt cacgaccttt cagcatatac
     2521 catgcgctga taccgaggat gaacatcgcg ccagtcacat aaccagaagc tacggtgtga
     2581 acgaatttca cctgagcaac cgggttaagc accagctcgg agaagctcac catctccata
     2641 cgcatagttt caaagttgaa atcggacgcg attgggtttt gcatccagcc gtttgcaacc
     2701 agaatccaca gtgcggacag gtttgaaccg agcgccacca gccaggtgac acacatatgc
     2761 tgaactttac ccagacgatc ccaaccgaag aagaacagac ctacaaaggt ggattcgagg
     2821 aagaaggcca tcagaccttc gattgccagc ggcgcaccga agatatcccc tacatagtgg
     2881 gaatagtaag accagttagt cccgaactgg aactccatgg tcagaccggt agccacaccc
     2941 agagcaaagt tgataccaaa caacttgccc cagaacttgg tcatatcttt ataaatctgt
     3001 ttgccggaaa ggacgtagac cgtttccata atggccagca ggaacgccat accgagcgtc
     3061 agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg
     3121 actatatcta acatcatgac tccttgctca tcgcatgaag actccgagag tgaaccccgt
     3181 tagaaagggt cacacgcatg ccccaataca aa
//
LOCUS       DAPQZD010000293         3190 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPQZD010000293 DAPQZD010000000
VERSION     DAPQZD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.299"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            54..545
                     /locus_tag="RO454_004907"
     CDS             54..545
                     /locus_tag="RO454_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0936596.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            548..1815
                     /locus_tag="RO454_004908"
                     /pseudo
     CDS             548..1815
                     /locus_tag="RO454_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            complement(1958..>3190)
                     /locus_tag="RO454_004909"
     CDS             complement(1958..>3190)
                     /locus_tag="RO454_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936597.1"
                     /translation="CFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQLAYT
                     LGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIP
                     AGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLAL
                     LAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIG
                     IARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFH
                     DRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLG
                     GDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIAL
                     WTLIKVKDEEPQVQLATS"
BASE COUNT          917 a          652 c          774 g          847 t
ORIGIN      
        1 taaatgctaa tattatggct atcatagcca taaaaagata aaatattatc actatgggta
       61 aaaaaacagc accgttactc ccttatacga atcagatgct tagtgaattt ggtgagcggc
      121 tgaaacttgc tcgtttacga cgcaggctta ccgcaaagca agttgctgaa cgtgctggga
      181 tgtcgccaat aacattgcgc tctctggagt caggaagtgc aggggtcacg ataggggcat
      241 atttgtctgt gatgcaggtt ttgggcctgg aaacggattt aaataagctt gcagctgaag
      301 acgacttagg gcgtcagctc caggattccc gtttgattaa aaaaagaggg ctgtctcgtg
      361 tatcaagtaa gaatgaagaa ggttctcaga cagcctcatt gcagatgagg cacgagaaag
      421 aaggtcatct caccacggat ttaacagcac tggcagaagg ctcgacgcca tctattacag
      481 gagaaacatc aacctctctt gctgcactat taaaaccgat aaaaaaaccg ttaaaaggaa
      541 attaattatg gcattaatcg gagtgtatgc ggattgggaa ggcctggatg gtcctgcgcg
      601 tattggttat ttacacagtc gtcgcactcg ggcgcgtgag atatttgagt ttgagtacga
      661 taaaaaggcg cttgcagatc cttcacttaa tttcattcag ttagatcctg aaataatgtt
      721 gtatgaggga gcacagtacc caattcctcc aaaggataaa tttggtgcat ttagtgattc
      781 ctgcccggat cgttgggggc gtatgctaat gaagcgtcgt tttgagaggg atattcgaga
      841 cgggttatgt gataaagact cccaccttta tgagtctgat tatctactag gtgttcatga
      901 tctttatcgg gttggggctc ttcgatacaa acgtgaagac gctggagagt ttcttgataa
      961 tcgcattgat gttgcagccc caccatttac cgaaatagcg agtctggaaa gggttagccg
     1021 ggcaattgaa gaagatcctg ataataaaga gtaatggggc aaaaatggtt aagaatgcta
     1081 atcgctccgg ggggatcatt agggggggca cgtccgaaag ccagtgttgt tgatgaagct
     1141 gggcatctct acatcgctaa atttcccagt gtaaaagatg agtatgatgt tggtggatgg
     1201 gaaatggtcg ttaatgcact tgctgttggc tgtggactga atgttgcacc tgctcaggcg
     1261 cataaatttg ccagcaatta ccattgtttt atggttcgtc gttttgaccg cactaatgcg
     1321 ggaaggcgtc tgcattttgc ttcggctatg acactaacca ggcatcagga tggtgaagat
     1381 gcctctacag gtgtgagtta tctggaatta gcagatgttc ttatcagaca tggcgcgcag
     1441 actaatactg accttaagga actctggtcc aggattgttt ttaatattct tgttactaat
     1501 accgatgatc atttacgcaa tcatggattt atcttgatac caggaaaagg ttggcggctt
     1561 tctgaggcat atgatttgaa tcctgtggtc aggtctgatg gtctaaaact caatattacg
     1621 gaaaatgaca atgcacttga tttggaatta gctagagaag ttgcagagta ttttcgactt
     1681 agtttgacgg aagctgatga cattattgct aattttagag ggattgtcag tcagtggcga
     1741 atcattgccg agcgtcttcg attacctggt cgtgagcaag agttaatggc agaggcgttc
     1801 aggggggcaa tttagaatag ttatttcgat atattcgcta aatcataaat ttgcgaagca
     1861 atgccagtag atactgaacg ttttggaaga gacgtttaat ttatgagaaa aatagaaacg
     1921 ctacaaagct aaattatagc gtttctatgt aaaataatta gcttgtcgct aactgtactt
     1981 gtggttcttc gtctttaact ttgataagcg tccacagcgc aatggcgcca ataatgtcaa
     2041 agaaagccag ggcaataaag aacggcgcga agccgataat gtgaacgaat gccccgataa
     2101 acaggttgaa aatcaactga ccgctccatg cacaggctcc tgccataccc acagcggtag
     2161 ctacttcgtc ttttttgaat aggtcaccgc ccagagttgc tgcaacagta gaaagacatt
     2221 gatgcgagaa tgcgccaatg ctaatcagta atacggcgat gtagggattt tcgacaatac
     2281 tcaccagacc aatcgtcatc ataatgacgg ctgcaatagt gaaggtaatt cttcgtgagt
     2341 taattaaact cacgccgcga tcgtggaaga acttcgcgac aaaaccactg gctaaacagc
     2401 cgagatcgcc cagcaggaaa ggtagccaga cgaacatggc aatttctttc aggctaaaat
     2461 gcagcgtttc gacgaagaaa atcggtaccc agaagttaat ggttccccat gccgggtcag
     2521 cgagaaaacg cgcgatgcca atcccccaga aattacgttg gctgaggata tgcttgattg
     2581 acgttttttc tttcttatct gttttaagat gctgttcttg cccagactca atatagtggc
     2641 gctcttcatc agaaaggcgt ttagcatctt ttggatcttt ataacaaaag aaccaaaata
     2701 aagccgcgag taaagcaagg ctacctgaga caataaacgc aaattgccag ctatgaaaca
     2761 taatgcacca ggcaatcaac ggtggagcaa gcatcgcgcc gagtgaggtg cccatattga
     2821 aaacacctgt cgccacgcca cgctcttttg ccgggaacca tgttgatgcg gtttttacac
     2881 ccgcaggaat tgccgatgct tcgctgaaac ccatcagggc gcgcataaat gccagacctg
     2941 accatgttcc tgtcaaggca tgccccattg tcgccaggcc ccagattgcg gcacatatcg
     3001 caaaacttaa cttcagaccc acggtatcaa taaagaagcc cattatcggt tgccccaatg
     3061 tataggccaa ctggaatgca ctgacaatcc atgaatattg ttctttggtt attccggttg
     3121 tctccataat cgttggagcc gcaataccta acgccgttct gtccagataa ttgattacag
     3181 tgataaagca
//
LOCUS       DAPQZD010000294         3070 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPQZD010000294 DAPQZD010000000
VERSION     DAPQZD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.300"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            44..577
                     /locus_tag="RO454_004910"
     CDS             44..577
                     /locus_tag="RO454_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0936598.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGYSVVEIPDITANRRADDSGKWMFKDGAVI
                     KRVYTEEELRLQTENQKKILLQQAREKTQFWQTQLTLGIITDSDRQQLMNWMRYVQQV
                     ETTDTSVLPVTFPEPPE"
     gene            complement(608..1135)
                     /locus_tag="RO454_004911"
     CDS             complement(608..1135)
                     /locus_tag="RO454_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0936599.1"
                     /translation="MQHLKNITAGNPKTIEQYQLTKKAGVIWLYTEDGKNWYDELKNF
                     QDDTLKIAYDQKGIIRCIEKDVSTLNPDGLSVVELPNITANRRADISGNWKFLDGKVV
                     KREYTKQELQQQAELQKAALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVMV
                     SRVDTAKPEWPQKPE"
     gene            complement(1151..2119)
                     /locus_tag="RO454_004912"
     CDS             complement(1151..2119)
                     /locus_tag="RO454_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936600.1"
                     /translation="MTGELKIGTVNALRIFNDAFGLIFRRSEDFLHFIPTAEGQGENG
                     DIGPLRPFAINLRTGAISVSHGAKIDGGLALGTDNALGGNSITLGDNDTGIKQGGDGV
                     LLFYSNGQLAFGLQPASADFYKRVAYIHQGIIPDGSGAFADQLNNATAPFVQTQFAWN
                     PTPGGLYVPIVKGLSIRNGQGYPGAVSFGYLLTEQYGFPVPCIHMRGDGGNDALWQFN
                     PNDKSFISPGALIAGGVRYNTDGNIFGGCWGSNLNDYLNSSFIRNVRLGGRRSDTLYR
                     GGLCEPGNGHVTTGLQIIGEVDGDDWMVSRPLQKYISGNWYNVEQA"
     gene            2245..2427
                     /locus_tag="RO454_004913"
     CDS             2245..2427
                     /locus_tag="RO454_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0936601.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            2473..2571
                     /locus_tag="RO454_004914"
                     /pseudo
     CDS             2473..2571
                     /locus_tag="RO454_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2626..>3070)
                     /locus_tag="RO454_004915"
     CDS             complement(2626..>3070)
                     /locus_tag="RO454_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDY0936602.1"
                     /translation="LISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQF
                     DSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRI
                     YNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          865 a          691 c          666 g          848 t
ORIGIN      
        1 aagtatataa atggcacctg gtataatgta gcgagcgttt aatatgatgc acttaaagaa
       61 cataaaagcg ggtaatgcta aaacactgga acagtatgag ttaacaaaga aacacggagt
      121 tatctggctt tactctgagg acggaaaaaa ctggtatgag gaagtgaaaa actttcagcc
      181 agacaccata aagattgttt acgatgaaaa taatattatt gttgccataa ccaaagatgc
      241 ctccacactt aaccctgaag gttatagcgt cgttgagatt cctgatatta ccgccaaccg
      301 gcgtgctgac gactcaggta aatggatgtt taaagatggc gcagtcatta aacgtgtata
      361 taccgaagaa gagttgcgcc tacagacgga aaaccagaaa aaaatattat tacagcaggc
      421 cagagaaaaa acgcagttct ggcaaacaca gcttactttg gggattatca ctgattcaga
      481 cagacagcaa ctgatgaact ggatgcgata cgtgcaacag gttgagacaa ctgatacctc
      541 ggttcttcct gtaacattcc cggaaccacc agaataagaa aaggcccgta cgggccttaa
      601 tttgttgtta ttctggtttc tgtggccatt caggctttgc agtatccaca cggctgacca
      661 taacgctgta gcgttcccat gattccagtc gtgtgcgttc ctcatcagtt gccatattca
      721 gtctgacagc acgttccagc ggttgaatca cagactccgc ttcggaaagc aaagcggctt
      781 tttgtaactc tgcctgctgt tgcagttcct gttttgtata ttcccgcttt actactttac
      841 catccagaaa cttccagttt cccgagatat cggcgcgacg gttggctgtt atattcggta
      901 actcaacaac acttaacccg tcagggttaa gtgttgatac gtctttctca atacaacgaa
      961 taatcccctt ctggtcataa gctattttta aagtatcatc ctgaaagttt tttaattcgt
     1021 cataccagtt tttaccgtct tctgtatata gccagataac gccagctttt ttcgtaagct
     1081 gatactgctc aatggttttg gggtttccgg cggtaatatt tttcagatgt tgcatatatt
     1141 ctcctgatgg ctatgcctgt tcaacgttat accagttacc agaaatgtat ttttgtagtg
     1201 gtcgtgacac catccagtca tctccatcaa cctcaccaat aatttgcaat cctgttgtca
     1261 catgaccatt acctggttcg caaagtcctc cgcgatataa tgtgtcagaa cgtctgcctc
     1321 ccagacgcac atttctgata aaagaactat tcaggtaatc atttaagttt gacccccagc
     1381 acccaccaaa tatatttcca tcggtgttat aacggacgcc acccgcaata agagcacccg
     1441 gtgaaataaa ggatttatcg ttcgggttaa actgccataa agcatcatta ccgccatcgc
     1501 cacgcatatg aatacatgga accggaaatc catactgttc tgtcagtaaa tacccaaagc
     1561 tgaccgcgcc gggatagccc tgtccattgc gaatggacaa gcccttaact atcggcacgt
     1621 aaagaccacc aggagtggga ttccaggcaa actgcgtctg aacaaaaggc gcggtggcat
     1681 tattcaactg gtctgcaaat gcgccacttc catcaggaat tattccctga tgaatatatg
     1741 caacccgctt ataaaaatca gcagatgcgg gttgaagccc aaatgccagt tgtccatttg
     1801 aatagaataa aaggacaccg tcgccgccct gtttaatacc agtgtcgtta tctccgagag
     1861 taatggaatt accgcccagt gcgttatctg taccaagcgc cagcccacca tcaattttgg
     1921 ccccgtggct gacagatata gcacctgttc tcagatttat agcgaatggc cttaatgggc
     1981 cgatatcacc gttttcgcct tgtccttcag ccgtcggaat gaaatgaaga aaatcttctg
     2041 aacgacggaa aataagaccg aaggcatcat tgaaaattcg cagcgcattc accgtgccaa
     2101 ttttcagttc tccggtcatt ttatcgccgg aacgctgaac ggcgttacca gccttgttta
     2161 ccgtttcctg taaaccgagg ttttagataa tggcggtttc tggcctgcat ggcatgattt
     2221 gtgcttttgg acgggagatt cagcgtgctg attggctatg taagggtatc aacaaatgac
     2281 cagaatacag atctgcaacg aaacgctctt gtttgtgcag gatatgaaca aatatttgaa
     2341 gataaattaa gcggaacaag gacaggccga cctggattaa aacgtgcttt aaagcgcctt
     2401 caaaaaggtg acgcactggt tgtctgaaaa ctggactgac tggttcactg tatgcggcat
     2461 cttgtcgtgc tggtggtgga agcctagaaa aagtatcggg tgttactgaa ccgtgttgat
     2521 acatcaactg cacctgatat tgagtggcct acgaaccctg tcagggagta atcattggga
     2581 ttatgccgca gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga
     2641 agcggaaaac attactacag taccttgaac cttggtttta acattctcga aatgctctga
     2701 gagtatatgt gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt
     2761 gtaaatgcgc atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt
     2821 ggctccgtat agagttccat cgtcagttaa ggcctgcgcc gcattgcgta ttacacagct
     2881 ttttgtagat atatttccag gcaggcagtg aagaaggtaa aacatggaaa tggaatcaaa
     2941 ttgaccatgt aacgccgcgg gataaggttc aaaaacatca tggctaattt tatgtttaat
     3001 ttttgattcc ccagcccttg tagatgccgc gttcaggcta gcttcgttca aatccattaa
     3061 agatatcaga
//
LOCUS       DAPQZD010000295         3038 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPQZD010000295 DAPQZD010000000
VERSION     DAPQZD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.301"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            185..1036
                     /locus_tag="RO454_004916"
     CDS             185..1036
                     /locus_tag="RO454_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDY0936603.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1063..2052
                     /locus_tag="RO454_004917"
     CDS             1063..2052
                     /locus_tag="RO454_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDY0936604.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2083..2976)
                     /locus_tag="RO454_004918"
     CDS             complement(2083..2976)
                     /locus_tag="RO454_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0936605.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          792 a          782 c          735 g          729 t
ORIGIN      
        1 ttttatggac attcattttt attcatcgga ctgtaaattc cggggatatc gaacttgcac
       61 gactaatctc aaccctcaaa cagatttatg aattttgttc ataacccctg acaaatcctg
      121 ccgtctaatc gagctcttcc ctttacgtta tgcttctcat gaacgataac ggaaggataa
      181 caccatgcaa aaagtaaaac tgaacaacgg tattgaaatg cccctgttgg gctttggtgt
      241 atttcagatg accgatgccg ccgaatgcga aagagcggtc attgacgcta ttaacagcgg
      301 ttatcgactg attgacaccg ccgcctctta tcaaaatgaa atccaggtcg ggaacgcact
      361 gaaacaaagc ggtatcgccc gtaacgaact ctttgtcacc acgaaactct ggttgcagga
      421 taccagttac gaaggggcca aagcccagtt cgaacgctct ctgaatcgac tgcaacttga
      481 ttacgttgac ctgtacttga ttcaccagcc atatggcgat gtccacggag cctggcgtgc
      541 catggaagag ctgcaacagg ccggtaaaat tcgcgctatt ggcgtcagta acttccatcc
      601 tgaccgactg gcagacctta tcgcttttaa taacgtcgtt ccggcggtaa accaggttga
      661 agttaaccct ttcaatcagc agctacaggc cgttccctgg atgcaaagtc gtggcattca
      721 gccagaagcc tgggcaccgt ttgcggaagg gaaaaatggt ctgttccagc atcctgttct
      781 cacggcaatt ggcgagaagt atggcaaaag cgtgggccag gttgttctgc gctggatcta
      841 ccaacgaggc attgtttcac tggcaaaatc agtgcgaaaa gaacgcatgg aagagaacat
      901 caacattctc gattttgaac tcagccctga agatatgctg caaattaccg ccctggatac
      961 cgcaaccagc gcgttcttct ctcaccgcga tcccgcaatg gtggaatggc tgactggccg
     1021 caaacttgat gtttaagccg cgataaaaga ggtattcatt caatgcaaaa acgttatctg
     1081 ggtaaatccg gactcgaagt ctccgccctt gggctcggtt gcatgggctt aagccacggc
     1141 tacggcccgg cgaccgatac ccgtcaggct atcgaactta ttcgtgctgc ggttgaacgt
     1201 ggtgtcacct tcttcgatac cgccgaagtg tatggtccgt atctgaatga agaagtggtc
     1261 ggcgaagcct taaaaccgtt tcgtgaccgc gtggtaattg ccactaaatt cggttttacc
     1321 ttcggcgatg ataataagca gcagatttta aacagccgtc cggaacatat ccgtgaagcc
     1381 gttgaaggat cattacgtcg tctgaaaaca gatgttattg acctgctgta tcaacaccgt
     1441 gttgatccag atgttcctat tgaagatgtt gcaggaacgg tgaaagacct catcgcagaa
     1501 ggtaaggtca aacatttcgg cctgtctgaa gcaggagcac aaaccattcg tcgtgcacat
     1561 gcggtacaac ctgtcaccgc cctgcaaagc gaatattcca tgtggtggcg tgagcctgaa
     1621 caggaaattc tgccgttgct ggaggaactg ggaattggtt ttgtcccctt cagcccacta
     1681 ggtaaaggtt tcctgacagg ggcgattaag ccgggaacca ctttcggcaa agatgattac
     1741 cgcagcaccg taccgcgttt cgcagcacaa gccattgaag ccaatgaaaa actggtcacc
     1801 ttgttaggtg aactggctgc agataaaggg gtaacgtctg cacaaatcgc actggcatgg
     1861 ctattagcac aaaagccgtg gattgttccc atccctggca ccactaaact gaaccggctg
     1921 gaagaaaacc tggccgctgc tgacattgtt ctttcgcaga aagatacgca gcagataacc
     1981 gaggcgctgg aaacaattaa aatcgtcggt gaacgttact cgcctgaaca tcaggcacgc
     2041 gtaggccgct aatacccaaa cgggtccagg ataaaggacc cgttattcgg tgtagcgaag
     2101 cgcatcgatc aacagcgcaa aagctggcgg atgctgcttg cggctggggt agtaaagata
     2161 atacccagga aaagttggac accactcctg taacacctga ataagcttgc ctgactttat
     2221 atactcctga accatgtctt cagggacaca agcgatccca aaccctgata acgcggcatc
     2281 aattctttct ggcaatggac tacacgtcag ttgcccatca accttaacgc gaagcggttt
     2341 cccttctcgc tcgaactccc agtggtaaag cccaccggca gttggcaaac gcatgttgat
     2401 gcagcgatga ttttgtagct catgaggcgt ttcaggcgca gggtttgcag caaaatacgc
     2461 cggagctccc accacagcca tccgcatatc tggtccaatc ctgactgcaa ccatatcttt
     2521 atcaacattt tcacccagtc gtatcccggc atcaaaacgg ccttcaacaa tatcgacaaa
     2581 accgttatcc accaccagtt cgacattgat ttcgggatac tctctgagaa aaggttttag
     2641 cttcggccag accagacttc gcgcggcatg ttccccggca gataaccgaa tattcccgga
     2701 gggcgtgccg ttcaattgaa taagtgattc cagttcctgt tcaagctcgt caatacgcgg
     2761 ttcaaggcag gcaatgattc tctcccccgc ttctgtggga gccacactgc gggtagtccg
     2821 tgtcaggagg cggatattga gcctttcctc cagagccttc attgcatggc taagtgcaga
     2881 ctgagagacg ccgagtttgc ccgctgcttt ggtaaaactt cgctctcttg ccaccacaag
     2941 aaagatttgc agttcgttga agttttcttt gagcattggc gatttccttg tagaagcatc
     3001 ctcttcacag acactcatga aggggggcat agatgcta
//
LOCUS       DAPQZD010000296         2970 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPQZD010000296 DAPQZD010000000
VERSION     DAPQZD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.302"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            493..1062
                     /locus_tag="RO454_004919"
     CDS             493..1062
                     /locus_tag="RO454_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDY0936606.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHSRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            1229..1612
                     /locus_tag="RO454_004920"
     CDS             1229..1612
                     /locus_tag="RO454_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936607.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            1609..1794
                     /locus_tag="RO454_004921"
                     /pseudo
     CDS             1609..1794
                     /locus_tag="RO454_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1833..>2970)
                     /locus_tag="RO454_004922"
     CDS             complement(1833..>2970)
                     /locus_tag="RO454_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDY0936608.1"
                     /translation="AEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIA
                     GPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRQLSPLEEALHG
                     YVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTCVRDDRNAGSTLAPAVWFAYSPDRKG
                     IHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEE
                     ALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKA
                     FAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYS
                     LIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          640 a          869 c          813 g          648 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcctc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacatactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatcgctcc
      301 ctctccacgc cttatccgta acctgttttt tacattaaaa aaacaacgcc gggaattcaa
      361 tcgtcagtcc ggcgatgacc gttcgggtta tcacagagtg cctgagacag tgtcctgccg
      421 gaggtcacct caggggctct gcgcattttt tacgacgtgg ttattccgta tgaaccatac
      481 ggagatttaa ccatgaccta caaatacaac cccttctggc agcaacgtat tcgtgagacg
      541 gtgcggcacg cactgaatgt tcattcccgc ctgacggcat tgcgggttga cctgcgtttc
      601 ccggatgtac cggcagcaac ggacgcagct gtgatatccc gcttcatcaa tgccctgaaa
      661 gcccgaatcg acgcttacca gaaacgtaag catcgggaag gtaaacgcgt gcatcccaca
      721 accctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcac
      781 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gcgattaccg cgctccggga
      841 tcactggccg ggatgattaa acaggcgtgg tgcagtgccc tgggagtgga tgccggacgt
      901 tatgacacgc tggcacattt tccggtcagg ccggcggtgt ggctggaacg cgatgatgac
      961 accggctttc agcaggtgct ggagcgtgct gactatctgg cgaaggagag taccaaagct
     1021 tacggcaccg gtgagcgcaa ctttggctgc agtcgtggct gagcccgaca gacgacttat
     1081 ctgaccttag tgtgatgacg tgttcacagc gaccacactg attttccctt ctgagactga
     1141 actgaaaacc gccattcaca cctgaccacg ctgcccgtac cgggacggct gttgcctgtg
     1201 tgtcgtctgg cgcagaggaa catatactat gtacgcaaaa tcttttctcg cacttgatgg
     1261 caacggacgt ctgaccggtg cccgtaccgc acaaactgct ccttatgccc actacacctg
     1321 ccacctttgc ggcagtgcac tcagatacca cccgcaatac gacaccgaac ggccctggtt
     1381 tgaacacact gacgacgggc tgacggagca cgcgcagcaa tgtccttatg tcaggccgga
     1441 acgcagagaa atacggttga ttaaacgtct gcagcaattc gtcccggatg ccttacccgt
     1501 ggtacgtaaa gccagctggt actgcaggca atgtcaccac gattattatg gggagcagta
     1561 ctgcacacac tgccagaccg ggcgtttcag tgaggagggg gtagcagaat gaacggtata
     1621 aaaacggggc tggggatcac gccgggtgag cacattatca gtgccgactc tgcgctcagc
     1681 agaaatatcc ggcattgttt ctgcctttcc tgtcatggcc ggttgattct gcagacagat
     1741 gctcaggggg cgtggtttga gcatgattta catgccctga gtgagcaaca gaagggtaag
     1801 cgtgcagcaa gaaccgtatt gacggggatg tgttattcag tcggcagtgc tacgcgccag
     1861 gggagcagtt cgccgacccg gtttatcggc cagtcggcta tgacgtcaag gacatagcgg
     1921 aggtagcttt ctggctccac tccgttcagt ttgcacgtcc cgatcaggct gtacagcagc
     1981 gctccccgct ctcctccatg atccgaaccg aagaacaggt agtttttgcg gcccagactg
     2041 accatccgca acgcattttc agcgatgtta ttgtccgcct cagcccagcc atcatctgca
     2101 tagtacgtca gcgccggcca ctggttcagg gcgtatgcga acgctttcgc cagttctgag
     2161 tgtcgcgaca gggttttcat cttttcacgc agccagcttt ccagggattt cagcagcggt
     2221 ttcgttttca actgacgttc ggcaaggcgc tgctccgccg tcattcccct tatctctgcc
     2281 tcgatggcgt acagttcgcc gatccgtttc agcgcttcct ccgtcagggc tgacggggtg
     2341 cgaacgtgca catcgtggat tttacggcgg gcgtgagccc aacaggcggc ttccgttatc
     2401 cggccatccc ggtacagctc gttgaacccg gcgtatgcat ccgcctgcag tacaccactg
     2461 aaccccgcaa gatgggtctg cggatggatg ccttttctgt ccgggctgta agcgaaccac
     2521 accgccggcg ccagcgttga cccggcgtta cggtcgtcac gaacgcaggt ccataaccgc
     2581 ccggtcttcg ttttcttatt gcctggcaac agcaccggga caggcgtgtc atcagcatgg
     2641 agcttaccgt cagtcagcac atagccatga agcgcctctt ccagcggaga cagctgccgg
     2701 cagcatgcat ccacccagcc cgacagcagt gaacggctca gctccacacc ttgccggccg
     2761 tatatttctg actggcgata cagcggggtg tgctctgcat acttcgaggt cagcacgcgg
     2821 gccagcagcc ccggtccggc gataccccgc tcgatgggcc gcgaaggtgc aggtgcctgc
     2881 acgatggcat cgcactgagt acaggcatgt ttttcccgta ccgtccggat aacccggaag
     2941 gcgctacgca tcaactccag ctgttcggcg
//
LOCUS       DAPQZD010000297         2963 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPQZD010000297 DAPQZD010000000
VERSION     DAPQZD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.303"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(72..845)
                     /locus_tag="RO454_004923"
     CDS             complement(72..845)
                     /locus_tag="RO454_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDY0936609.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(847..1371)
                     /locus_tag="RO454_004924"
     CDS             complement(847..1371)
                     /locus_tag="RO454_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDY0936610.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPS
                     GTYSLRQELIRTGD"
     gene            complement(1406..2602)
                     /locus_tag="RO454_004925"
     CDS             complement(1406..2602)
                     /locus_tag="RO454_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDY0936611.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(2616..>2963)
                     /locus_tag="RO454_004926"
     CDS             complement(2616..>2963)
                     /locus_tag="RO454_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDY0936612.1"
                     /translation="ETPQDVEITRVYTMNNTDRHQDHIAVYQASMVACRYIPQILGYE
                     TPSTCLSFLPQVFESVDEVHFNKKILALQKHKSQIHRRYIQPEYIIAQAKFRGQQVSH
                     SMCEGFVVHKMVL"
BASE COUNT          826 a          711 c          657 g          769 t
ORIGIN      
        1 tgtttattat caaaccgagt tatagctttt ctctgaaaac taccgaataa aatatactga
       61 tagactgctg cctagaattt ccagatcagg tgggctgcaa gttgcagacc gttataatca
      121 tcggttgggg tgttgaacca cactttacct gccgtcaacc cgagattaag ttgctcagtc
      181 agcggttgaa ttcgttgcaa actgatgctc ttcatgctgc cgtagcgtgt ttctggcgtc
      241 cagtcgtagg tgtaagcacc tgttccgcgg cttagccata gttgcgtata accagcaccg
      301 cgcgggtcct tcagacgcac ggaaatcata ttgctataac tactacctgc atcgctggag
      361 tcataatggg tatagcggta actggtgatc accgggccag tatagagtga tacgccgcct
      421 tgccaggcat tgacttcgac atcatcgtaa tatttagtgt agcgataacc ggtcgtgaaa
      481 agcgtttttg gcaacagggc taggttaata tcctgtcgaa aatcctggcg ggcaaagacc
      541 ggcgtattat ccgcaaaggc aatacccgtt cgggttgtca gccagttatt ccatttgtac
      601 cagactgcac cctggccgcg agtggcattg aaatgttcag tttcataatc acgacgcccc
      661 tgggaaagat taaaaagcag cgttgcgttt tcaacttttg ctaccagttc agcatacgct
      721 aaattacggt tgccatgttc gccagaataa tcggtaaaat cgtagcccgc ggtaatggaa
      781 gttaacccgg cataagcggg caatgcacta aaaaaaaacg gcactaataa cgtatgcttt
      841 atcattttaa tctcctgtac ggataagttc ttgtcggagt gagtaagttc cactaggtaa
      901 attacgttct acaatcacgc gcgggccaag ttgtgtgttc ggagaaataa ttcgcccagg
      961 caaaataata acttgtacgc caaggcgtga acgcttcccg atataacaac ctaatttatc
     1021 gcatccggta gcgataattc cctctggagt tcgaacagac acgggttgtt catccagacg
     1081 atgattactg gtacgtactt gtgcgcccaa atatgcctgg tttgcaacta ccgagtcggc
     1141 aataaagcat tgcggaccaa tcgttgcttc cgcttcaata accgcatttt taatttcggt
     1201 ggcaaaacca atttttacgc cattgctgat tattgtgcca ggacgaataa acgcataatt
     1261 acctatcagg cagtttgcgc caatcactac cggcccctga ataacggcac cgtggcaaat
     1321 acgggtattc gcgccgataa caacctcacc ggcactttca tcaataatca cctggtctgc
     1381 aatctgaaca tttttctctt tagcattaag cttcagatag tttttcagtt cctgtgacag
     1441 aaggttggga ataagcggtt gacgcttcca ccagtgcgga atagcgggtg ggtttgtcgc
     1501 gctgaggttc gcgtccagta aaaaaggcaa taagtccata aataatccag atggcataag
     1561 ctaataatac atacacaacg gaaagcggtg ctaaaggcac caacaaccag caacgatgaa
     1621 accaggcgct aaatgcacca ataacacaaa ctacgccgac ccagataagc gggaacattg
     1681 ccaacaccac accatgtggc ccggtggtga tgaattcatt aaaccaggta gtgagataaa
     1741 taccaacgcc ataaagcaca accaacagca taggaaatat actgaagata ccgaagcggc
     1801 taaataaaag tcttttatgc aggcgcatac agaccgcgta gcccacaatc caacgccgcc
     1861 agcggcgcca ctcctcacgc ggactgttac attcctgtgg gtatacgatg cagcgattcg
     1921 cctgccgaat acggtagccg tttgccacca acgtccaggt gagatcaagg tcttcgactt
     1981 tagtacgatc agagaaaccg aacttacgca atacatcagt acggaacatc ccgcaggcac
     2041 cgctgataat aaacggtgca ccgcccagaa actgctgtag cgtgcgcttc ataacaatca
     2101 tcggcaactt tacggtggcg cggatatgtg gcagcagacc tgcccctttt aacgcggtgg
     2161 agggaattcc gccaacggca tcagcgccgc gctcaatttc tgccagcata tagcccattc
     2221 catcattatc aggtggaacg aaagtatctg catcacttaa aaatacttga tcacacgtgg
     2281 cgtattttag tccatgcatt agtgcgccac ctttaccggt gtttttttgc gtgacggcaa
     2341 caaagcggtc gccccatttg cgtttgactt ccgccatgac cgcttcggta ttgtccgtgg
     2401 atccgtcgtt aacgcaaatt acccggcaaa aataagggtt acgcagcaga ttatccagtg
     2461 actgcgccag acacgggcct tcgttataag ctggtataat ggcgtcaata cagcctttct
     2521 tttgacaggg tttacgtctt agcgtactta aaaaccatag caacattgct atggtcatga
     2581 agataaataa caaagttttc atttctatta ctccgttaca acaccatctt atggacaaca
     2641 aaaccttcac acatagaatg actaacctgt tgtcctcgga atttggcttg agcaattatg
     2701 tattctggct gtatgtatct tcgatggatc tggcttttat gcttttgtaa tgctaatatt
     2761 tttttattaa aatggacctc atctacagat tcaaaaactt gaggtaaaaa tgacagacat
     2821 gtacttggtg tttcgtatcc tagtatttga ggaatataac gacaggcaac cattgatgcc
     2881 tgataaacag caatgtgatc ctgatgcctg tcagtattat tcatggtgta aactcgagta
     2941 atttccacat cctggggcgt ttc
//
LOCUS       DAPQZD010000298         2825 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPQZD010000298 DAPQZD010000000
VERSION     DAPQZD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.305"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            41..244
                     /locus_tag="RO454_004927"
     CDS             41..244
                     /locus_tag="RO454_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936613.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            264..494
                     /locus_tag="RO454_004928"
     CDS             264..494
                     /locus_tag="RO454_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936614.1"
                     /translation="MFVLIAGVNVHNEYYVNRIAGIAGYAGRAVELIDETTRKIDLLS
                     DQERKKADVNDADIFLMLKAFVEMGFEISLHK"
     gene            complement(1111..1593)
                     /locus_tag="RO454_004929"
     CDS             complement(1111..1593)
                     /locus_tag="RO454_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936615.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIETQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(1944..2318)
                     /locus_tag="RO454_004930"
     CDS             complement(1944..2318)
                     /locus_tag="RO454_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936616.1"
                     /translation="MRLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICFLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVGQLKE
                     LVEDLIKNHDFHNNRLTFCAWW"
     gene            complement(2436..>2825)
                     /locus_tag="RO454_004931"
     CDS             complement(2436..>2825)
                     /locus_tag="RO454_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936617.1"
                     /translation="VKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQASTIV
                     RKGDASGGEYATQYQSILDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASSLNTN
                     TTWIGQYGLRITSGIPADTLIPESVFA"
BASE COUNT          745 a          638 c          611 g          831 t
ORIGIN      
        1 atcaagcgcc cactatgggc gctttaaagg agctttaaaa atggtcagct atgacaagat
       61 ccgcgcagaa tatcgcgcaa agtatcgcgc ttataaactg gaattaatcg atgatttgat
      121 cgcgcaacgc gaccaattaa attttacgtt ttctgatttg cttaacagca agcgagattg
      181 caagcgcaaa cgtgaatact tacgtttaag cgcattaatc ggaaaacttc aaaattctat
      241 ttaatttttt aaggagctta atcatgtttg ttcttatcgc tggcgttaac gtccataacg
      301 aatattatgt taatcgcatc gctgggatcg ctggttacgc tgggcgcgca gtcgagctta
      361 tagatgaaac aacgcgcaaa attgacttat tgagcgacca ggagcgaaaa aaagcagacg
      421 tgaacgacgc tgatatattt ttaatgttaa aagcgtttgt agaaatggga tttgaaatca
      481 gtttacataa ataaaatcga gcgcccacta tgggcgcttt tttcgtttcc aatacttcca
      541 ccataacgcg ccatcattgg cgcgtttttt attgtcttta gctcactcca acaacataaa
      601 aataagcgcc aaaataacgc catagacgcg cttttatatc cttgtcagta catacccatt
      661 acttaacaca ttaacgcgct taaaatgagt tatattgcgc tataacgtat ggctaatcat
      721 taatttttat tcttgctatg tgatccgcgt ttatttgtcg gcgcggatcg gcattttgtt
      781 ttgttccgta tccgctcgcg tattatgtgc gcgtgatttt tcacataatc acaccactta
      841 atcacgtatg tgattacgct acgaaagaaa tctggatgtc tcaggcgacg aaagtcatca
      901 taattttccc cgctcacctg tccgacaacc gctggttgga ttccaccagc ttcccgatgt
      961 tttttctaca caaaggcgaa tgcagccgtt tcccgaaaaa atcctggccg ttccccgtcg
     1021 gctcatgaat acgttcctcg ccgtttctga aaatttccct gcggcagctg gtggctatag
     1081 agaaagggcc gtttctggcc ctcttctcag ttacacgcca tcaaggatgt ggatgcggtt
     1141 gcttgcgtat acattcagca taaagttagc gcaaaacagt ttccatgtat caacgccagc
     1201 ggtatacgta atgtttttgc atttaattgc attgttggcg atccgcatcc cctgcgatac
     1261 tgcttcatcg tcggaaaatt tgaacgacga ttgagtttca atccagacag aaatctgcgt
     1321 agcgaactca atcagcttgg actggcagaa tcgcccggag cgcaccggaa agacgaacgt
     1381 tccgaatcca gaatttacca catacgcttt ctcaaatacc cgcgagtaac gacgattgcc
     1441 aatgatgtcg cgtgcaataa tgcatttttc ttgtgctgac agttctaccg tctcattgtc
     1501 gcgccatgca ccaagtactc gtttttcaat gtcagagaac gttacagcga tattgccatg
     1561 tgcgggtgcg tttacagtag cgataaaatt catgattaat tccttatcgt aaataacaaa
     1621 ttgttttctt gttggcgtta attatcgttg tacgaataag gcgtcaaagt ggaaagttac
     1681 ggtagccgga cgggaacagg tgggtttgtc ggttgcctgg aggtaagagg ttggtgtttt
     1741 tagcctgcgg gaaacaagat ggtcatttaa ggccaccaat gctgatggcc ttaataatta
     1801 tcgcccgatt acgctaagga tcttttcctc aactgatttg ttgttgcgac taaactgcct
     1861 tgcgtatctg atgacagaag aggcgttttg ctttctgacc atctcggctc tttccttcag
     1921 gcgtttcttg aaatcgccca tatttaccac caggcgcaga aggtcagtct gttattgtga
     1981 aagtcgtggt tcttaatcag atcttccaca agctctttca actgccccac atcatgccag
     2041 taaccttcat cgtactcctg actgccgaag aaaaaacctt cccgggtagg caagtactct
     2101 tcgcaattac tttcgtttat gtgcatcaaa tgagccttca gaaaacaaat gtcattcatc
     2161 gttaattcta aaagctcaca attttcgact tcacctacgt tgcgctccat ccacccaacg
     2221 agcgcattga acttacggaa gtaagcaacc tgctttctgg atgcctcgtt attcagatcg
     2281 tttttaggct gtgtctcgat atagatatca agtctcatga tggtttcctt attaagttgc
     2341 ttcagtgaaa tcattttcac aaatcagata aggcagaaaa taaattgtta tcgggcttaa
     2401 taaaatggcg cggtttacgc gccatttaaa gaggattaag cgaatacgct ttcagggatt
     2461 aaggtgtcgg caggaatgcc agaggtaatg cgcaggccgt actgaccaat ccaggtagtg
     2521 ttggtattca ggctggaggc gaatacggtg ttaaccatat tcatcatggt ttcaaacacc
     2581 tcttcatcaa cctggcggaa gtagttctcg attttcagca gcagcgggtc agttgcatcg
     2641 agaatggact ggtattgagt cgcgtactcg ccgccggagg catcaccttt acgcacgatc
     2701 gtactggcct ggacttcggc accagtttcg gtgttgtacg taacaacggt gatttttgcc
     2761 acattcttac cttcggcggt ttcagaggca tagtaaacat caactgtcag tttttcgcgt
     2821 ttaac
//
LOCUS       DAPQZD010000299         2795 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPQZD010000299 DAPQZD010000000
VERSION     DAPQZD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.306"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            184..909
                     /locus_tag="RO454_004932"
     CDS             184..909
                     /locus_tag="RO454_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDY0936618.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            867..1544
                     /gene="bglJ"
                     /locus_tag="RO454_004933"
     CDS             867..1544
                     /gene="bglJ"
                     /locus_tag="RO454_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDY0936619.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="RO454_004934"
     CDS             complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="RO454_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDY0936620.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCDDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2511..2747
                     /locus_tag="RO454_004935"
     CDS             2511..2747
                     /locus_tag="RO454_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDY0936621.1"
                     /translation="MLQRTLGSGWGVLLPGLLITGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          739 a          594 c          709 g          753 t
ORIGIN      
        1 tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc aggagtatgc
       61 tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt tgctggaaac
      121 atttaaggaa tgaatatcct gaataaacca tcataatttt tagacagtgg atgtggagga
      181 aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatacc tgttatgcaa
      241 gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat atccagcgcc
      301 tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat tgctgattta
      361 gccggtgaaa gtgaagatcc acgttctgtt tgtgaacatt attattcttt aatctcacaa
      421 tatcgggaaa ttcactgggt tttcatggtg tcacgctcct ggtattccca ggcagtagaa
      481 ctgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga gaatctggtc
      541 aagaccgtac gttccggcaa tacgcacgca gagcgtatca gcgccatgct gacctctccg
      601 gcaatgactg aaactcatga ttttagctat cgctccgtca ttctcactct ttcagagcgc
      661 aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc attgcttaag
      721 aaaagtaata aaactatcag cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac
      781 agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga acttaacttg
      841 ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag agaaatgtcg
      901 cactcataga aaaatgcgtc atgagtagta tcggtattga gagtttattc agaaagtttg
      961 cgggcaaccc ttataagctc catacctata ccagtcagga gtcatttcag gatgccatgt
     1021 cgcggatctc gtttgcggcg gtcatttttt ctttttctgc catgagaagt gagcgcagag
     1081 agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg cgtttagtta
     1141 ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca ccgctggacg
     1201 gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt ttgtcattaa
     1261 atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac caaagccgga
     1321 cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac tcaatgacac
     1381 aaattgccga gcagcttaaa cgcaatatca aaacgatccg cgcgcataaa tttaatgtga
     1441 tgtcgaaact gggcgtcagt tctgatgcag gattgttgga ggccgcagat attctgttat
     1501 gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca tgatgccggg
     1561 tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct gcacatccgg
     1621 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccgtcgcgca gtaccacggt
     1681 acgccagagt ggattatctt caccgttcgt gagcgttttc tcaaaaaaga tggcatggcg
     1741 cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtgagat accagttgat
     1801 caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg cttctagtgc
     1861 ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     1921 tggtgttgcg tttttatcgt cacacacatc gacccagaaa caggcggcac ggccggtttc
     1981 gtgaaactca gcatggaaat gttccggcga cacatctaat gctttttcct gcgtcagtag
     2041 cgccagcatt aatggtggca ccatcaggcc gatataccat tgtgcccata gtgagatcag
     2101 cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg aatagaccgc
     2161 gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg cgttaagcgg
     2221 agcgggttca tccaggcgga taaactcgag caaatgttca cgatgtttgg cgatcgtcgc
     2281 acgtacggct tgtgcaagcc cagcatcctg tggctggaga tgcgttcgcc agataacatc
     2341 ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa tgataatgat
     2401 tgctaatcct agcgataggt ttatccgata gcaagggatt tctctggctt gcaaatgata
     2461 aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc atgttgcaac
     2521 gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattaca gggctgatgt
     2581 atgcggattt atcgccggat caatggcgga ttgtcattct gatgggattg gtattgacgc
     2641 cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg tgcctggcgc
     2701 ttattgctgg catcatgctg atgataatga atttgaatca ggggtgataa aacagtgaag
     2761 agacaagaaa agaagtaaag ataattggtg cgagg
//
LOCUS       DAPQZD010000300         2771 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPQZD010000300 DAPQZD010000000
VERSION     DAPQZD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.307"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(119..>2771)
                     /gene="chiA"
                     /locus_tag="RO454_004936"
     CDS             complement(119..>2771)
                     /gene="chiA"
                     /locus_tag="RO454_004936"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDY0936622.1"
                     /translation="SSLPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKA
                     KANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSNTPASNTPANGGSATPAQ
                     GTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVR
                     AATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQ
                     EGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNPGS
                     CSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEI
                     YHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEIDN
                     GGVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSA
                     STVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKTQSYSNEEL
                     NNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTP
                     KKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGM
                     QNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLD
                     FDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSD
                     AKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHAQLKGLYGNKSD
                     AEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNL
                     SPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          659 a          722 c          658 g          732 t
ORIGIN      
        1 caattgcagg aagactcttt tttaagcaaa gaatagaaat atgaataaaa ataatgggct
       61 accaaaagta gcccattgac aaaaaatgcg gcgataccgg aaggtatcgc caacacattt
      121 attgcttagt aaacggcgcg aagatttcgc taaatgcgta cttcggagcc tgttctttag
      181 tcagaccgtg gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc
      241 agataccaac cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag
      301 agagatagaa cacctcaccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca
      361 tggcgtcaat ttctgcatca ctcttattgc catagaggcc tttcagctgc gcatgcaggt
      421 tggcaatcgc agacgttgca cacttaccgt ggatgttttg gccttcggtg tttgcagact
      481 gacaaatagc gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt
      541 tcgctttggc atcgctcagg acattcatcc cttccggcgt caggcctgtc ggcagaattg
      601 gcaaggtata ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct
      661 tcacagcaag gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat
      721 cgaagtccag gactctgagg ttcaggttat caacgatgtc ataataatgc tgcattagat
      781 cgtcaacgtt cttacaggaa gctgccagcg gggcattatt agcaccaccg atagacagca
      841 tcacatcacc gccagcctca cgcagcgctt tgattttgct gtactgagcg taattctgca
      901 taccgtaagc ggtcccccat gtcggcagac aggtgttcgc atctttactc accacaaacg
      961 ccagcgtgaa gtggttgacg ttatgattct tagccagcgc agccagatcc gggatcgtat
     1021 tcagcgtaaa gtcgacatat ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac
     1081 ctacgcgctc tttggttccg gtccagtcaa caaagacacg ccacgggttg ctgtcagaag
     1141 gagaaacttt ctgcacttta ctctgagaaa tgtagttctc accgttaaag cgaatcagcg
     1201 tatcactggc ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc
     1261 tatagctctg agtcttacct aacggcttcc acgggcgact attgctaccg gtggcgtttt
     1321 ggttcgcaac cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc
     1381 catcaacaac aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg
     1441 gttcgtattt cttccacggg ttatcgccgc cggggacaaa gccccatgcg tcttgatcga
     1501 cagaggtttt ataatgtgca tcgttataat ctacgatgct gttcgcgcta tacgctttgc
     1561 tggcctggaa accatccaca ataatgacgc cgccattatc aatttcacag gtagtcgggt
     1621 taccgtactg actcatttcc gcagcggtag ctgtacgcac gagacgccat gggttattgg
     1681 agttttctac actggtgcca cgcgggcaat cttcagaacc aacccaccag gcattctgat
     1741 aaacattacc attgaagata acatggtaaa tttcgctgcc ttcctggcca ctccacgcct
     1801 gcagtgagta atcgttactg ctatccgctg gtgttgatgg ctcgttgtta tccggagttg
     1861 gagtaactgg agtttccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat
     1921 tattatccgg cttaacggaa caggagcctg ggttaccata ctgagtgatt tctgttgccg
     1981 ttgccgcgcg aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc
     2041 aatttgccgc gcctacccac caggcgttct tataaatgct gccgttgtag ataacgtacc
     2101 atgtagaaga accttcctga cctttccagg ctacaacagc atagttgtcg tttgacttat
     2161 cagctggggc tggctgaaca tcaggggccg gtttaacgtc cggtgccggt ttcacatccg
     2221 gtgcaggctg tggtgctgaa gtacaagact gtggattact ggtttctgag atttccgttg
     2281 ccgtagcggc acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg
     2341 ggcagttaga agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat
     2401 accaggtttg accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag
     2461 tgccctgtgc tggtgtagcc gaaccgccat tagcgggcgt attagatgct ggcgtatttg
     2521 aagttgaaga agatgagctt ccgctctttt cgcaggaaag tgtattgcca aactgactaa
     2581 tttcagcagc tgttgcggta cgctttaaac gccacgggtt agttgcatca tttgctttcg
     2641 cttttcccgg gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga
     2701 aaataacctg atatttatta ccaccttgtt gattattcca tgcttccatt gccaatgctg
     2761 gcagagagga a
//
LOCUS       DAPQZD010000301         2745 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPQZD010000301 DAPQZD010000000
VERSION     DAPQZD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.308"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            44..2323
                     /gene="ydeP"
                     /locus_tag="RO454_004937"
     CDS             44..2323
                     /gene="ydeP"
                     /locus_tag="RO454_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDY0936623.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2571..>2745
                     /locus_tag="RO454_004938"
     CDS             2571..>2745
                     /locus_tag="RO454_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDY0936624.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRMARDLTHF"
BASE COUNT          738 a          642 c          672 g          693 t
ORIGIN      
        1 ctattacaaa tcctaataat tcatttccac acaggataag cagatgaaga aaaaaattga
       61 atcctaccag ggagctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg
      121 taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg
      181 ctttgactgt ccgggatgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg
      241 tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa atgcctcttt
      301 ctttgctgag aatacggttc aatcattact tacctgggga gatcacgagc ttgaggctgc
      361 gggacgtctc actcagcctt tgaaatatga tgacgtcagc gactgttaca agccattaag
      421 ctggcaacaa gctttcgacg aaattggcgc tcgccttcaa agctatagtg atcccaatca
      481 ggttgaattc tatacttcgg ggcgcacttc caatgaagct gcctttcttt atcagctttt
      541 tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc atgaaccgac
      601 cagtgtggga ttggcagcga gtatcggtgt gggtaaaggg accgtgttgc tggaagactt
      661 tgagaaatgc gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat
      721 gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcta tcaaccctct
      781 acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa
      841 ctctgagact cagttagcca gtgcctacta taacgtgcgc attggtggcg atatggcgtt
      901 gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg
      961 gccctcattg ctggatgacg aatttattca aacgcatacc gtcggctttg acgagctacg
     1021 ccgtgacgtg ctcaattccg agtggaaaga tatcgaacgt atttctggac taagtcagac
     1081 acaaatcgcc gaacttgctg atgcatatgc cgctgccgaa cgaaccatta tctgttacgg
     1141 aatggggatc actcagcacg aacatggtac ccagaacgta cagcaactgg tcaatctgct
     1201 gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gaggtcactc
     1261 taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc
     1321 tcgcctgggt gagcgctatg gcttcacccc acctcatgca cctggacatg ctgcaatagc
     1381 cagcatgcaa gctatatgta cggggcaagc tcgagcattg atctgcatgg ggggcaactt
     1441 tgcactggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatctggc
     1501 ggtacacgta gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat
     1561 tctgccggtt ctgggacgta gcgagattga catgcaaaaa agcggtgcgc aggcggtaac
     1621 cgttgaggat tcaatgtcga tggttcatgc ctcgcgtggt gtgttaaaac ccgccggtgt
     1681 aatgctgaaa tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac taccccagag
     1741 cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc
     1801 tgtgctgcca gagtttgccg actataacca gcgcatccgt catcctggtg gttttcacct
     1861 gataaacgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta atttcattac
     1921 cagcaaaggg ctgttagaag atccttcttc agcgtttaac agtaagctgg tcatggcgac
     1981 agtacgcagc cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg
     2041 tgtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt
     2101 aaaaaacggc gaaagagtta atcttattgc actcacgcca gacggtaaac gtagctcacg
     2161 ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta
     2221 tttcccagaa tcgaatcaca tgctaacact tgataaccac gatccattaa gtggcattcc
     2281 tggctataaa agtattccgg ttgaattaga accatcaaat taattctcct cctcatttct
     2341 tctgctgtca tccgcacagc agaagaattc ctcattgact attatcgcgc aatttgctca
     2401 catggattaa attaaactac acactataag atataaacct ctgcctacag ttgtaagaaa
     2461 ctccgctcag tactgaagtg ccagtcctat ttcctctttt atccagcctg ttatattaat
     2521 catactgatt aacgattttt aacgttatcc gctaaataaa cacatttgaa atgcatgcga
     2581 ccacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg tctgtaattg
     2641 tggttgtctt gttactgaca cttacgttga tagccatttt ttcggcaatt gatcagctgg
     2701 gcatttcaga aatgggtcgc atggcaagag atcttacaca tttca
//
LOCUS       DAPQZD010000302         2629 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPQZD010000302 DAPQZD010000000
VERSION     DAPQZD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.310"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            133..744
                     /gene="ruvA"
                     /locus_tag="RO454_004939"
     CDS             133..744
                     /gene="ruvA"
                     /locus_tag="RO454_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDY0936625.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            753..1763
                     /gene="ruvB"
                     /locus_tag="RO454_004940"
     CDS             753..1763
                     /gene="ruvB"
                     /locus_tag="RO454_004940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDY0936626.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
     gene            1873..2629
                     /locus_tag="RO454_004941"
                     /pseudo
     CDS             1873..2629
                     /locus_tag="RO454_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          657 a          626 c          705 g          641 t
ORIGIN      
        1 gaataaatta tactgcgcca tttttcagat catcgagaca cctcgcaagt tttcttcatc
       61 cttcgctgga tatctatcca gcattttttt atcatacagc attatctttg attcattacg
      121 caggagcgtc atgtgatagg cagactcaga ggcatcatca ttgaaaaaca acccccgctg
      181 gtgttaattg aagtgggcgg cgtaggctat gaagtgcata tgccgatgac ctgtttttat
      241 gaactccctg aagcgggtca ggaagcgatc gttttcaccc actttgtggt gcgtgaagac
      301 gcgcaactgc tgtacggttt taacaataaa caagagcgca cgttgttcaa agagttgatc
      361 aaaaccaacg gcgtcggccc gaagttggcg ctggcgatcc tctccggaat gtcagcgcag
      421 cagttcgtta atgccgttga gcgtgaagaa gtgggggcac tggtgaaact gccgggtatt
      481 ggcaaaaaaa ccgccgaacg tttgattgtc gaaatgaaag accgatttaa aggtttgcat
      541 ggcgatctct ttacgccagc cgccgacctg gtactcacgt ctcctgccag cccggcgacc
      601 gacgatgctg aacaggaagc ggttgctgcg ctggtggcac tgggctataa accacaagaa
      661 gcaagccgca tggtgagcaa aatcgctcgc cctgacgcca gcagtgaaac attaattcgc
      721 gaagccctac gcgccgcgtt atgaggtaaa ggatgattga agcagaccgt ctgatttctg
      781 ccggtaccac tttgccggaa gatgtggcag atcgcgccat tcgccctaaa ttactggaag
      841 agtatgttgg tcagccgcag gttcgttcgc agatggagat tttcatcaaa gcagcgaaac
      901 tgcgcggcga tgccctcgat catctattga tttttggtcc tccggggttg ggtaaaacta
      961 cgcttgccaa tattgtcgcc aatgaaatgg gcgttaattt acgcacgact tctggtccgg
     1021 tgctggaaaa ggcgggcgat ctggcagcaa tgctcactaa ccttgaacca cacgatgtac
     1081 tgtttattga tgagatccac cgtctttcac cagtggtgga agaggtgttg tatccggcaa
     1141 tggaagatta ccaactggat atcatgattg gtgaaggtcc ggcggcacgc tccattaaaa
     1201 ttgatttgcc gccgtttacc ctgattggtg caaccacgcg cgcaggttcg ctgacatcac
     1261 cgttgcgcga tcgttttggt attgtgcaac gtctggagtt ttatcaggtg ccggatctgc
     1321 aatatatcgt cagtcgcagc gcacgcttta tggggcttga gatgagtgat gacggcgcgc
     1381 tggaagttgc tcgtcgtgcg cgcggtacgc cacgtattgc caaccgtctg ctgcgtcgag
     1441 tgcgtgattt cgccgaagtg aagcacgatg gcaccatctc ggcagatatc gctgctcagg
     1501 cgctggatat gttgaatgtc gatgctgaag gtttcgatta tatggaccgc aaattgttgc
     1561 tggcggtaat cgataagttc tttggcgggc cggtaggtct ggataacctg gcggcagcca
     1621 ttggcgaaga acgtgaaacc attgaggatg tactggaacc ttatttgatt cagcaaggct
     1681 ttttgcagcg tacaccgcgt gggcgtatgg cgacgacgcg ggcgtggaat cactttggca
     1741 taacgccgcc agaaatgtcg taagtcggat tggtcgctcg tgccgcatcc gacactggca
     1801 gtgtgcctga tgcgacgctg ttgaactgca ccccaaatgt tggacataat ctgacatctg
     1861 aaggtgcagt ttatgaaaca ttcatttgaa gtaaaacttg ccgctgtaaa tcattacctg
     1921 gctggtcatg caggcatcat ttctacggca aaactctttc aactttctca caccagcctc
     1981 tcacactgga ttaatctttt tcttcttcac ggtcctcggg cactggattg cagacacaag
     2041 cgtagctatt ctcctgaaga taaactttgc gtggttcttt atgctctcgg gcattctgag
     2101 tctctacccc gggtagcagc ccggtttaac attcccagcc acaacacggt taaaaactgg
     2161 ataaaaggct accgcaaatc tggtaatgag gcctttatca ggcgcaggaa ggagaaaagc
     2221 atgacccgtt ctgatgatac ccatgaaaac gaggcaaaca tgacgccgga agagatgaag
     2281 aatgagctgc gttatcttcg cgcagaaaat gcatatctga aagccatgca ggagcatctt
     2341 ctggaaaaaa agcgccagga gctggagaaa aaacgaaagt catccagagc ctgaggtacg
     2401 gacactgcca gtctgacctg ttaaaggccg cagggctggc cagaagcacg ctgtactacc
     2461 agctgtcact gcagaaggct aaggacaagt atgctgatgt gaaacagctc atagcctcca
     2521 tcttccatga acaccgggga tgttatgggt accggcggat ccattgtgag cttcagaaaa
     2581 gaggtcttaa gttcagtggc aaaacggtgc gaaaactgat gcaacagct
//
LOCUS       DAPQZD010000303         2598 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPQZD010000303 DAPQZD010000000
VERSION     DAPQZD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.311"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..1049)
                     /locus_tag="RO454_004942"
     CDS             complement(join(<1..791,791..1049))
                     /locus_tag="RO454_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0936627.1"
                     /translation="MTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDVHANQIKQ
                     WKDQLLEGATGVFGDETKAEPSGPTIDVKTLHAKIGELTLENGFFIRCARQGGIAGRK
                     EMIDREHKLSVVRQAKLLGFSRGSVYYLPRPVSDGDLALMRRIDELHLDYPFAGSRML
                     QGLLRGEGLETGRRHVATLMKKMGIKAIYRRPNTSKPAPGHIRKLAVTRPNQVWAMDL
                     TYIPMARGFVYLCAVVDWFSRKVLSWRLSITMEAAFCIEAVEEALARHGRPEIFNTDQ
                     GSQFTSIDFTAVLKRSQIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEAR
                     AGIARYLNFYNTRRPH"
     gene            complement(1141..1683)
                     /locus_tag="RO454_004943"
     CDS             complement(1141..1683)
                     /locus_tag="RO454_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0936628.1"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVP
                     IPASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQL
                     LHIFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDS
                     GAHTSDELAAMVLDRLDQRT"
     gene            complement(1696..2556)
                     /gene="aac(3)-IIe"
                     /locus_tag="RO454_004944"
     CDS             complement(1696..2556)
                     /gene="aac(3)-IIe"
                     /locus_tag="RO454_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IIe"
                     /protein_id="HDY0936629.1"
                     /translation="MHTRKAITEAIRKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDKARRTWPPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRNGEVAWKTASEYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGATPIVPAHE
                     AAQRSCEPSG"
BASE COUNT          514 a          795 c          719 g          570 t
ORIGIN      
        1 atgtgggcgt ctggtgttgt agaagttcag atatcgggcg atgccagcgc gtgcctcgga
       61 cacagtcttg taggcatgga ggtagacttc ctcgtatttg atcgaacgcc agagccgctc
      121 gacgaagaca ttgtctcgcc acgcaccctt gccatccatc gagatggcaa tctgtgacct
      181 cttcagcacg gcggtgaagt cgatggaggt gaactgcgat ccctggtcgg tattgaagat
      241 ttccggcctg ccatgacggg caagtgcctc ctccaccgcc tcgatgcaga aggctgcttc
      301 catcgtgatc gacaaccgcc atgacaagac cttccggctg aaccagtcca cgacggcgca
      361 cagatagaca aatccccgcg ccatggggat gtaggtcagg tccattgccc acacctgatt
      421 gggccgggtg actgccagct ttcgtatgtg ccctggcgct ggtttcgagg tgttcgggcg
      481 gcggtagatc gccttgatgc ccatcttctt catcagcgtg gcgacgtgtc gccgcccggt
      541 ctccagacct tctcctctca agagcccttg caacatccga cttccggcaa aggggtagtc
      601 gagatgcaat tcgtcaatcc ggcgcataag ggccagatcg ccgtcagaca ccggacgagg
      661 cagatagtag acgctgccac ggctgaagcc gagaagcttc gcctggcgca cgacggatag
      721 cttgtgctcg cggtcgatca tttctttccg cccagcaatc ccgccttgcc gagcgcaccg
      781 gataaaaaat cgttctccag tgtcagttcg ccgattttcg cgtgcagcgt tttgacatcg
      841 atggtcggac ccgacggctc cgctttcgtt tcatcgccga acacacctgt cgccccctca
      901 aggagctggt ctttccattg cttgatctgg ttggcgtgca catcgaactg ctgggacaac
      961 tccaccagcg tctgctcacc tcggatggcg gcaagtgcca ccttcgcctt gaaagccggg
     1021 ctatggttcc ggcgcggtcg tctcgtcatg gtctctcctg ttcccggcat ctaagccgaa
     1081 gtcaggcaga aactccactt atccccgctg tgcagatttc ccgagccagc tctaaggcga
     1141 tcaggtgcgc tgatcgagtc tgtcgagcac catcgctgca agttcatcgc tggtgtgtgc
     1201 accactatcg agaacacggg ttgtggacgg aagccgttcc cttgcggcca agcatcgggc
     1261 gacattcgct aatcgccact ctcgaatctc cgcattccga ttcgggtcag gatgcatggt
     1321 ctggttcgag atccggtgac gcaataggtc ctcgttaagc atcagaaaga tgtgcagcag
     1381 ctgatcgtcg atctgcctta acccgtcgag tatctcagtc agatagtccg ggtgcacgag
     1441 cgtcattggg atgatgatgt cctgcgagta attcctgcga atctcgctga ccgcagcgat
     1501 cgtaagtccc ctccacaagg ggagatcctg atagtctccg ctcgctggta tggggaccgt
     1561 ttctttcacc acgaacccga tttcctcagg gtcaaagatc agcgatttcg gacgccgatc
     1621 gcgcagccgc tcagcgagag tcgtctttcc ggcgccgaaa ggtccgttga tccagattat
     1681 cattgtcgac ggcctctaac cggaaggctc gcaagagcgc tgggcggctt cgtgtgctgg
     1741 cacgatcgga gtggctccga agtgcttctc aagataggtg acgccgaacg tcacgatgtc
     1801 ctgcgcgtcg aacaggtagc actgagcaaa gcccacgaca ccttctcgat ggcgaccgag
     1861 cttcacgtaa gcatttgcta tagtttcgac cgcatccggc tttccttcga tagcaaagca
     1921 atcgagaatg ccgtttgaat cgtattctga tgccgttttc caggcgactt caccgtttct
     1981 tccaagcatc ggcatctcat acgtcaccca tcgtttgttg gggatatccg caaccgcctc
     2041 ggcgtagtgc aatgcggtaa cggagtttag cggcgcaccc aacagcaggg ccttcccgcc
     2101 aaggcggacg aaccgctcga cgggcgaccc ttcccccaag gcgtgaccga gttcgtgagg
     2161 ctccgtcagc gtttcagcta gcggaccaac cgcgaccatc gatgcatcgg ggtgcgcgct
     2221 gcgccgcgcg ccgggggctt gaaccagaaa ttgattcagc aggccgaacc cacggtaagt
     2281 cccggccgtt gcgggatcga acggcggcca ggtacggcgg gctttgtcat ccaaccgagc
     2341 gccattcaga gtctcctcgt agggtgatcg gtcccacgac gcgtatccca tcacagtgcc
     2401 agtcggccca accgcggagc gtaacgcggc aacgaccgtc tccgctcctc cttcgaccgg
     2461 accaatcgct ttaagtgagg catgcaccat caacaggtca ccggtttgga ctcctaattt
     2521 tcgaattgcc tccgttattg ccttccgcgt atgcatcgcg atatctcctc taaactgcaa
     2581 aacactatac ctatcgag
//
LOCUS       DAPQZD010000304         2570 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPQZD010000304 DAPQZD010000000
VERSION     DAPQZD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.312"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            350..493
                     /locus_tag="RO454_004945"
     CDS             350..493
                     /locus_tag="RO454_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDY0936630.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            506..715
                     /gene="cspE"
                     /locus_tag="RO454_004946"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             506..715
                     /gene="cspE"
                     /locus_tag="RO454_004946"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDY0936631.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            881..1690
                     /gene="rlmA"
                     /locus_tag="RO454_004947"
     CDS             881..1690
                     /gene="rlmA"
                     /locus_tag="RO454_004947"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDY0936632.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLREWLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1687..2253)
                     /gene="mntP"
                     /locus_tag="RO454_004948"
     CDS             complement(1687..2253)
                     /gene="mntP"
                     /locus_tag="RO454_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDY0936633.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          692 a          589 c          611 g          678 t
ORIGIN      
        1 tgatacccgt taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
       61 agtagaaaat gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac
      121 tgaacttatt gaactatgtt agaaaatacg tcagtttaag tatctgcctg aactggcaag
      181 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
      241 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
      301 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
      361 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
      421 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
      481 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
      541 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagatg tgttcgtaca
      601 cttctccgct atccagggta atggtttcaa aactctggct gaaggtcaga acgttgagtt
      661 cgaaattcag gacggtcaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
      721 tccactgatc tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag
      781 gctttttact ttgttttatc ttcgctccag tcgttcggat atttgcccgc cgcatgattc
      841 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact
      901 ttgccatcag cctcttttgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
      961 tgatatggcg aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga
     1021 tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta
     1081 tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa tggcttgatg aaaaggccac
     1141 ggcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ctgatgcgtt
     1201 gcccgaaatc accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa
     1261 acgctatccg caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac
     1321 cagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagtgcgagt
     1381 agtgaagccc ggcggttggg tcattactgc cacgccggga ccgagacact taatggagct
     1441 gaaggggctg atttacaatg aagtacatct tcatgcacct catgcagaac aactgaaagg
     1501 ttttacatta cagcagagtg atgagttgtg ttatctgatg cgtcttcgcg gtgatgaagc
     1561 cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg aagcctgaag tctggcaagc
     1621 actggcagca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
     1681 ttcttattaa ccgtggaagt gcgtccagag gatctggacg ccaataccga tcagtaccag
     1741 cccaccgaga atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat
     1801 ccctaatgtc gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt
     1861 aacctgcagg aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc
     1921 ggtggttacc agtagccaga aaccgtgtcg acggcgcggt tcttcatctt catcatctgc
     1981 gccacgaaaa ccctcaataa tcattcgccc gccgagaaat atcagcagca caaacgcaat
     2041 ccagtggttc cattcaagga caaaccgact ggctaacatg cccattcccc agccgatcag
     2101 cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt
     2161 cggtttatgg agggtggcac ctttaccgat tgatgcagca aaggcatcca tcgacatacc
     2221 aaacgcaaga agaacagtag cagtgatatt catgacaacg tcctgaccgg ggtatccata
     2281 aaacacatca tcgcccccag caaacatgca tacatgaaca acaagccgcg atggttttcg
     2341 cgttcatgca ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtgttcagt
     2401 ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat
     2461 cagctactcc ccaaatgagg gcgcaacctt aacatatttt gagaatataa gacaactgtt
     2521 ggaatatatt attttattgt tttattgata acgattttca tttagattta
//
LOCUS       DAPQZD010000305         2542 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPQZD010000305 DAPQZD010000000
VERSION     DAPQZD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.313"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            43..1257
                     /locus_tag="RO454_004949"
     CDS             43..1257
                     /locus_tag="RO454_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDY0936634.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            complement(1837..2049)
                     /locus_tag="RO454_004950"
     CDS             complement(1837..2049)
                     /locus_tag="RO454_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936635.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(2049..2384)
                     /locus_tag="RO454_004951"
     CDS             complement(2049..2384)
                     /locus_tag="RO454_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936636.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGTLTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
BASE COUNT          725 a          572 c          578 g          667 t
ORIGIN      
        1 gctgtaagcc gcgccacgac tggtctgaag tcatgttcat gtatgctgtc agcggtaatt
       61 gatagtctgt cagcggttca ccgtatgctg ccaactgttt ttggtattct gtcagcggca
      121 aatcaagtat gctgtcagcg gttgaatgac aaaggtgtcc acatgtccac aaaaaataaa
      181 aaaggtaaaa ataacaaaga agtagaagat aacatcgaca actttgaaga aggttccctt
      241 gaattgtaca caggggaact tgtccctaat agcaacaata cagtccaacc aattgcgttg
      301 atgcgactgg ggttgttcgt ccccacgctt aagggaacaa aatacagtaa acgtaacaag
      361 ccaaatgaga tcgatgcttc aaaggagctt gtccagctcg aagtagcccg ttctgaaggc
      421 tattccgata tcaagataac cggtcctcgt ctcgacatgg atcatgattt caaaacgtgg
      481 gtgggcgttg tgcgttcgct ggcagaatac ggcgaaccta acggtcgtgt cgagttaagc
      541 atcacgaaat ttgctaagtt ttgtggctat ccatcgtcac agatccgcaa aacactccgc
      601 gaccggctta caaacagtct tttgaagatc atgcgtacaa ctctgtcttt ccagagaacg
      661 tatgaagaaa agaacgtcga cggctctaac aagatctcgc ttctgatggt gcacctcatc
      721 aatagcgtgg actacaacga gcagaaagat acggtggtgt tctatgcaga accgaagctg
      781 gccgaactat atcgctttga ccataaggtt cttttgcagt taaaggttat caataagcta
      841 ccacgcaaag aaacagcaca ggctctgtac accttcatcg aaagtcttcc aaccaagcct
      901 gcgccggtat cgcttgctcg attgcgtgcg cgactcaatt tgagtagcag gaatgtcagc
      961 tcgcagaatc agactatacg taacggctta aaagctctcc aagatttggg ctatctcgaa
     1021 tacagcgaga ttaagcgagg gcggtcgatc tatatccaga ttcacagccg caatccaaaa
     1081 ctcaaagtcg caccgccaaa acctgaagac atcgaaccca aaaaaccaga tgagaaagct
     1141 ggggaaattg atgccaaaca gaacattatc aacaagataa ccgaactttc gcagaatttg
     1201 acgcctgaaa atatcaagat gattgagatc ctttccaata gtctcaaact gctttgatat
     1261 gctgtcagcg gtgaaatgta tgctatcagc ggctattttt ctgaaaagta tgctgtgaac
     1321 ggttaaagat atgctaccag cggtatagtg agataaacgt atgctgtgag cggtaaatca
     1381 tctatcacca ctcaatgctc tggcatacct ccccagtggt tactaacctc gaagagtgca
     1441 ttccatatag gtttgcgctc actaacattc tattgctatt gcgctgattg cttgtagtga
     1501 aaggcattta ttgcctgcta ctccctgata gaacgtatgc tgtcagcggt gttttattga
     1561 ggaatttttg tttggatatg ctatcagcgg taattaatgg tatatgtcag accttcgtat
     1621 gctgccagcg gtaattttgt tctggttata cccataccgg tacacattat tcatgcaatg
     1681 tcttaagtat gctgtcagcg gtaaaaaatc gagtaaagta tgctgtgagc ggtgagatag
     1741 actctcgata tgctatcagc ggcagttgat tgtgtatgct gtcagcggtg acgactatga
     1801 aatatgactg cggaacgtgc cgcagtcagt ttgcatttag tgtattttct tcaacagacc
     1861 ccaaagtgtt ccggcttttg tagtcagttt tcctgtttct ggatcgtaca tacgccattc
     1921 acgccgctgg tggacgatat acccatcttc acgttccaga cgctcaagaa tgccaggctg
     1981 cttgaaaccc ttagcacgcc agtatccgct tgttttctcg atttccagac ctgccagtgt
     2041 taatgccatt atccaacctc cttgcagtca tcgaaaaggt agctttgctg agaagcgtga
     2101 acgccataga agcccttgta tttgttgtat acgaagtggt cttctccgac gccggttaat
     2161 ttgccattac gatttgtgag atatggggat gacaacactc gatcgtcacg cacaacatag
     2221 aactggtcgc cgctgtctac gaccatcgct ccatatggtg cttttatgac gtcggtaaga
     2281 gtgccattct taacgtcggc tacagacatt tcacactgat aaacagtggt gtcggccaca
     2341 acgatcgaag aaagaaacaa cagcgcgaat gggatggtct tcatgctgcc acctctttga
     2401 ttacatcaaa tgctgaatta aatacgagca cgatagccaa agcgtttatg actacgtcgt
     2461 gcaccgggga aaagacttta cgaatgacac cagtgaaaat gcagtcgatg acaaaagcta
     2521 ttgaaacgat gagtaacaag ta
//
LOCUS       DAPQZD010000306         2540 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPQZD010000306 DAPQZD010000000
VERSION     DAPQZD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.314"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(20..580)
                     /locus_tag="RO454_004952"
     CDS             complement(20..580)
                     /locus_tag="RO454_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0936637.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKMLDTRT"
     gene            complement(622..882)
                     /locus_tag="RO454_004953"
     CDS             complement(622..882)
                     /locus_tag="RO454_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDY0936638.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            1431..2180
                     /locus_tag="RO454_004954"
                     /pseudo
     CDS             1431..2180
                     /locus_tag="RO454_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          708 a          537 c          605 g          690 t
ORIGIN      
        1 ttatatgcac taaaaaggat taagtacggg tatcaagcat tttaattaaa gcgttaacgg
       61 ttcctgaata gatatcatgt ggtggaagtc gatttaacat cagtgccccc tttagtgtag
      121 aacagactat aacggcgagc tcatccggaa gaagaactgt tttgaactct ccactattct
      181 gcccggaggt gataatatct ttaatcatct gtatctcctg atgtgccagt tgacttacag
      241 cctcctgtaa ttcaggagaa aactgatggc tgtcggatag catggcataa ataccgcaca
      301 tttcccctct ttcagcacaa ccagcaaatg catcaagata tgtcctgaga cgttgtacac
      361 ctgaagaaac attatggaga gttatattaa gtctatcaaa tacttctgtt ttgtattggc
      421 agtaggctat accaagatca gttttcctcg ggaaatggtg atgaatactg gcttttctga
      481 taccaatttc tttttccaga tctgcgtaac tgaaccccaa aaatccgcgt tgctggatca
      541 gagtatccgt tatgcgaagt atattctcat aagtagacac ggctttacct gttatgtatt
      601 aaagggacta ttatacatat tttagcgata gataatggca gttccatcaa gtttattttt
      661 tcctcccaat gaagtgattc ggaaatgaga acctcctgcc tgctccgctt tgtctttaag
      721 cgcagttgcc acctcttctg gtgtatatag attaccaaca gaaatatagt ctgacggcat
      781 caaaccaaca acgtcctgac gattaatttc atctgcagca gttgcgtagc ctgtcagaat
      841 gagaccaagg gcgacaatgg tagtgcagat aacataattc atgagcaatt tctctttatg
      901 tttgcgatac tacacatgct accttctgat tggtaggctt gcaagatgga tttatttaaa
      961 ataagaggga tgatttgtat caaattgttt tgtatttttg tgttttgtat cactgaggtg
     1021 aagttgtaag atctggccct ctgcgaggtt ggctctgtcg aaaggaacag gtgacgagag
     1081 ttaacatcgt tgccttggct gcatggggga tatgtagcta ccccctgata tttccgccac
     1141 ggaagccaat atgaacaaca ataacaatac gcctcgtttc cagtatgctt catgtcggat
     1201 attttgtaag cgctatgtac ttcccgggac gcttatcttt tagccgtagc tggtagtgca
     1261 ataaaagcct ggcagcaatc caggcctcta cgttctaaga atcaactgtc agtgcagagg
     1321 aatgcgcacg acgctctcgt tgttattggc ttttgccgtc gcaccgtcgt ttttcacggt
     1381 gacaaacagc gcgtgcgctt tgctatccgt cttatatcac tggtgctgac aatgtggatg
     1441 aggtcgcctt taaaaaagga catcgttata taacggtgat ctccgatcgc gatggtcgtg
     1501 cgctggcctt aacggacgat cgtggcacag agagcctcgc cggctatctt cgcacgctta
     1561 ctgacgggca gttgctggct atcaaaacgc tctcaatgga catgaatgca ggctatataa
     1621 gagcagcgcg tatccactta ccctgtgcgg ttgagaaaat cgccttcgac cgcttccatg
     1681 tagcaaagca acttggcgaa gtggttgata aaacccgtca gaatgaacat ccgcacctcc
     1741 ctgttgaaag ttggcgtcag gcaaagggga cccgcttcct gtggcagtac agcgataaat
     1801 ggatgaccaa atccaggcag gaaaagctga tatggctgcg tgcacagatg aagctgacaa
     1861 gccagtgctg ggcgctgaaa gagctggcaa aggatatctg gaacaggcca tggagtgagg
     1921 aaagacgtaa tgactgggag agatggctgg ctctggcggc caacagtgac gttcctatga
     1981 tgaaaaacgc cgcgaaaacg ataggaaaaa ggctttacgg gatcctgaat gccatgcgac
     2041 accgtgtctc aaacggaaac gcggaggcgc ttaacagtaa gatcaggcta ctgaggataa
     2101 aagccagggg ataccgaaat cgggagcgct ttaagctggg cgtgatgttc cactacggga
     2161 aactgaatat ggcgttctga gccttcccac catgatcagg gaaaacccat gtatcatcag
     2221 gttattatgt gagctggtaa atcagatttt gcatggactg aaatcatgct gttatttagg
     2281 gataaagaac cggctttttg tcccgccccc tcgccattaa tgaaaaaaag aatggaatgc
     2341 tccctctgag gagaagggca gtagaaaaaa gaacccgcca gcaaaaatat tggggatgaa
     2401 cagctttcgc tactcagatt gctggcgggt atagttcctc atgaaataaa aatactacat
     2461 aatcctttta atgcaaatga taatcattgt caattgtttg tgcgtgtttt cttcatacat
     2521 gattggtagt aaaggtgatt
//
LOCUS       DAPQZD010000307         2483 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPQZD010000307 DAPQZD010000000
VERSION     DAPQZD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.315"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            15..1487
                     /locus_tag="RO454_004955"
     CDS             15..1487
                     /locus_tag="RO454_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936639.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            1491..2321
                     /locus_tag="RO454_004956"
     CDS             1491..2321
                     /locus_tag="RO454_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0936640.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
BASE COUNT          626 a          413 c          607 g          837 t
ORIGIN      
        1 ttgtgaggtt taatatggac tcctgtattc agaaaaagca ggtttggtat aagcatcttt
       61 cacccagaca gtggaagata ttcggtgcgg catggacagg ttatttactt gatggttttg
      121 atttcgttct gatatctctg gttcttacag aggttcagca tgaattcggc ttgacgacaa
      181 tagaagcggc aagccttatt tccgctgctt ttatttctcg atggtttggg gggcttgcta
      241 ttggcgcttt atcggacaag atggggcgca ggatggcaat ggttttaagt atagtgctat
      301 tctctctcgg aacccttgcc tgtggtctgg ctcctggata tgcagtgatg tttattgctc
      361 gcattgttat tgggcttggt atggcaggtg aatatggctc aagtgtaaca tatgttatcg
      421 aaagctggcc tgttcatctc agaaacaagg caagtggttt tttaatttct ggattttcta
      481 ttgggggagg tttagctgca caggtttata gcattgtagt accactatgg ggctggcgtt
      541 ctcttttctt tgtcggcatg cttccaatat tatttgcttt ttatcttcgc aagaatcttc
      601 cggagtctga tgactggcaa aaaaggcaac aagagaataa acccgtcaga acaatggtgg
      661 atattctgta cagagaaaag aataaataca taaatattct tctgtcttgt attgcatttg
      721 catgtctata tgtttgtttt tcaggcgtca ctgcaaacgc tgctttaatc acagttatgg
      781 cattgtgctg tgcggctgtt tttatcagtt ttatatatca gggtatgggg aaaagatggc
      841 caacaggcat tatgttgatg cttgtggtta tgttctgttt cctctacgga tggccactgc
      901 aggcttttct tcctacatgg cttaaggttg atatgcagta ttcaccagaa actgttgctc
      961 tcatatttat gctggcaggt tttggctctg cagctggaag ttgtataggt ggttttatgg
     1021 gggactggct tggaacccga aaagcatatg ttatatcact tcttatcggt cagcttgtta
     1081 ttatccccgt ttttcttgtg gaccgggact atgtatggtt acttggcctt ctaatcttta
     1141 ctcagcaggt ttttggtcag gggattggtg ctctggtgcc gaaaataatc agtggttatt
     1201 ttaatgtgga gcagcgtgcg gcaggactgg ggtttattta taatgtaggt tcacttggtg
     1261 gagcatgtgc gcctattctt ggtgcagttg ttgcgtctca tacaagtctt ggtacagcaa
     1321 tgtgttctct tgcttttatc ttaacatttg ttgttcttgt ccttattggt tttgatatgc
     1381 catcacgtgt acagagatgg atccatccag aagctgcgct tgaatatgat actgtcgatg
     1441 gaaaaccttt ttatggagca agaaaaaaga atgttgcaga agaataacgg atgctatcag
     1501 ggaataaaaa aatgattcat tacagggaga atatatattc tcttgataat tatgagcgtt
     1561 acataactga cagttcgcta tttgagaata ctggcgatgg cataatatat acaggtattg
     1621 tgccccttat tacagttgtt gagcctgttg tatctaatca acatagtgtg ctgattatac
     1681 ctggaggtgg atacaggagg attgtttatg aaagagaggg acttgatgtt ggcagatgga
     1741 tagcaggtca gggatatacg gcatttattc tttcttacag aatgccctcc gttaatggtt
     1801 attattcctt tgcagatgcg aagaatgccc tgaactttat ttcttactgg gccggaatgt
     1861 cggagagaac atttaagagt gttggatgta tggggttttc ggcaggtggt catcttgctg
     1921 gatatattac tcatctccat tatgaggaag agacttctgt gcgtcctgac tggatggctc
     1981 tgatatatcc agttatatct cttgacagga gtattgctca tcagggatgc cggaaacgtc
     2041 ttctggatgc aggtgaaagt gaatattcag ccagtcttga aaatagtgtt acagagtata
     2101 tgccaccagt ctttattgtg catgcaacag atgataagga tgttccagtt attcattctc
     2161 tgagatattt taactctatt cgggataagg gacatcatgc agaagctcat ttttttgata
     2221 agggaggtca tggatttggt gtatccggaa aaaatggata tccgatagaa aaatggcaaa
     2281 gcctgcttct ggaatggctt gcggaacata agtttcattg agaaatcaaa ttacaagtga
     2341 taaatatatt taatgatgag gcatttatga aaaaatatta tccactatta actgtaattc
     2401 ttcttggttt acctttgtgc tctcaggcat taactcttaa tctacgtggg ggatacagaa
     2461 gcgccagtca cgcttatgaa acg
//
LOCUS       DAPQZD010000308         2447 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPQZD010000308 DAPQZD010000000
VERSION     DAPQZD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.317"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(7..607)
                     /locus_tag="RO454_004957"
                     /pseudo
     CDS             complement(7..607)
                     /locus_tag="RO454_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(696..1130)
                     /gene="uspF"
                     /locus_tag="RO454_004958"
     CDS             complement(696..1130)
                     /gene="uspF"
                     /locus_tag="RO454_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDY0936641.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1271..2404)
                     /gene="ompN"
                     /locus_tag="RO454_004959"
     CDS             complement(1271..2404)
                     /gene="ompN"
                     /locus_tag="RO454_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDY0936642.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          706 a          534 c          538 g          669 t
ORIGIN      
        1 ttgagattat tgtttccgaa aattgattat tatcagcgcg aaaagaaatg ctacagcaaa
       61 taagaggacg attgtcggcg ctggggctct gttgagaaaa tataataacc atacgcctga
      121 tattgttatt gatatcgtta cggcgataag taatgcctta tgaaagcgat gcatgatgag
      181 gacagcaata gaccctggag ctattaatag tatatagaca gaataattcc gactgctttt
      241 aatgtggcaa aaatggtagg cgataccatg cagagtaaac cgtaatgtaa taatgatatc
      301 tgtaatccgc tgacttgtgc ctgttgatag tcaagacaaa aattcctgaa aaaacgatac
      361 ctataatggt atctttcttt ataatgctat tatctttcag atagccagtt gcaattgcac
      421 aagacagtcc tacaataaat gcaccaattc ccagcggcat acctataatc cagactagaa
      481 ctatgccagg aaacattgcg tggctcattg catctcctat tagcgcccag ccttttaata
      541 ccagaaaaac tgataataat gcacagggga acgatattat aatagcgatt atcaatgcat
      601 aattcataaa tttaaattgt aatgattaga ggggaaaata tcagcacatt tgcgaaatga
      661 atcaaaaagc ccgcagcaat gtgcgggcgt tagtgtcagc gcacaaccag cacggagcac
      721 tctgcgtgac gcactacagc tgcggcgtta gaaccgagca gataagtggt gatatccggt
      781 cgatgggaag caatgatgat catatgagcg gggatcttct tcgccaattc cagaatgcgg
      841 tctttgggcg agccttcctc aacatggaca tgcactctgt cggttggcag tttaaatttt
      901 ttaatgatct cttccagttg cgatttggct tccgctttca ggtcatccat tgccggtaat
      961 tctgcggaat acgctaaacc cagagaggca tagtagggca gcgaaggtat taccgtcagg
     1021 aaatgaacct ctgcatcatc aatctttgcc tctgcctcaa cgtggctaat cacgcgttga
     1081 gttaattctg aatcggaaat atcgataggg acaagaatcg ttctgttcat aaaacctcct
     1141 gttttagtat ccgcataaag tgtaacgcca gatgacactt tttgtgtaat gacggagttc
     1201 acatttttaa tttagatcaa aggaggaaga ataagcagaa aaagcccgcc aaaacagcgg
     1261 gcagggggat ttagaactga taaaccagac ctaaagcgac aatatcatcg gtagagatgc
     1321 cgttggcagc gtagaaactg tcatcttcat ccaacaggtt gattttatag tcaacatagg
     1381 tggacatgtt tttattgaaa tagtaagtcg cgccaacatc ggcgtattta accagatctt
     1441 tatcatcaac acctgccgga ttgtctgcac caccggcagc gtgcaggtca cggcctttag
     1501 acatcaggaa agagactgcc ggacgcagac caaaatcaaa ctggtactgt gcagtgactt
     1561 caaagttctg ggttttgttt gcaacagcat aatcgctgtc gccaaacggg gtcatattac
     1621 gcgtttctga atacatggtt gccaggtaaa tattgttagc atcgtatttt agcccagcag
     1681 tccacgcgtc tgctttatca ccacccgccg cagtatggtt aacctggtca ttggtgcggt
     1741 cagaagaggt gtatgccgca ccagcgctaa agcccatgcc taaatcatat gttgtggaaa
     1801 gaccccagcc gtcaccgttt tcatggcgaa catcacgtcc gttgttggtg ccttcctgac
     1861 cattactggc accttcgttg ttaccttgat actgcaccgc gaagttcaga ccatttacca
     1921 gaccgaagaa atcagtatta cgataagtcg cgacgccatt ggcacgacca gtcataaagt
     1981 tgtctgcatt ggtataagag tcaccgccaa attcaggtag catatcagtc cagccttcga
     2041 tgtcgtacat tacgccataa ttacgtccgt aatcgaaaga accgtaatct gcaaatttca
     2101 gtccggcaaa tgccagacgg gtccatgact ggtttgttga agattcggtg ttgtttgcct
     2161 gaatattgta ttcccattga ccgtagccag tgagttgatc gttaatttgg gtttcgcctt
     2221 taaaacccag acgagcatag ctctggtcgc catctttcgc tgaattatca gaaaaataat
     2281 gcaggccatc aactttgcca tacagatcca atttgttgcc gtctttatta taaacttcgg
     2341 ctgcatgtgc agcacctgca gcgagcaggg caggaattaa gagtgccagt actttgcttt
     2401 tcattgaata aatcctttag ttattttatt tgcctttcat ccccatt
//
LOCUS       DAPQZD010000309         2408 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPQZD010000309 DAPQZD010000000
VERSION     DAPQZD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.318"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..393)
                     /locus_tag="RO454_004960"
     CDS             complement(<1..393)
                     /locus_tag="RO454_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDY0936643.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIM"
     gene            551..1564
                     /gene="intI1"
                     /locus_tag="RO454_004961"
     CDS             551..1564
                     /gene="intI1"
                     /locus_tag="RO454_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDY0936644.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1767..2117
                     /locus_tag="RO454_004962"
     CDS             1767..2117
                     /locus_tag="RO454_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HDY0936645.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2243..>2408
                     /locus_tag="RO454_004963"
     CDS             2243..>2408
                     /locus_tag="RO454_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0936646.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTG"
BASE COUNT          513 a          668 c          671 g          556 t
ORIGIN      
        1 cattataggg aatttgatat caccttcgac ttcaacgtga acagtagaca aatgaattat
       61 atctgctttt tcaataaggc tattatagat ttgacccccg ccagagacat atacatgatc
      121 tgtaactttt gatagctctt tcaaagcatt ttctattgaa ggaaaaacta ggacgttttc
      181 atttgagctt gaaattccgt tctttgacac tactgcatat ttgcgatttg gaagaacacc
      241 catagagtca aatgtttttc ttccgacaag gagccattga ttatatgtga gcgctttaaa
      301 gagtagttgc tcacctttta ctgaccacgg gatatcagga ccactaccga ttacgccatt
      361 ttctgacact gcagaaatca atgatatttt caatttaact cccttaatgg ctaactttgt
      421 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
      481 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc
      541 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
      601 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
      661 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
      721 cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc
      781 cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga
      841 tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct
      901 gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc
      961 ccagcttctg tatggaacgg gcatgcggat cagtgagggt ttgcaactgc gggtcaagga
     1021 tctggatttc gatcacggca cgatcatcgt gcgggagggc aagggctcca aggatcgggc
     1081 cttgatgtta cccgagagct tggcacccag cctgcgcgag cagctgtcgc gtgcacgggc
     1141 atggtggctg aaggaccagg ccgagggccg cagcggcgtt gcgcttcccg acgcccttga
     1201 gcggaagtat ccgcgcgccg ggcattcctg gccgtggttc tgggtttttg cgcagcacac
     1261 gcattcgacc gatccacgga gcggtgtcgt gcgtcgccat cacatgtatg accagacctt
     1321 tcagcgcgcc ttcaaacgtg ccgtagaaca agcaggcatc acgaagcccg ccacaccgca
     1381 caccctccgc cactcgttcg cgacggcctt gctccgcagc ggttacgaca ttcgaaccgt
     1441 gcaggatctg ctcggccatt ccgacgtctc tacgacgatg atttacacgc atgtgctgaa
     1501 agttggcggt gccggagtgc gctcaccgct tgatgcgctg ccgcccctca ctagtgagag
     1561 gtagggcagc gcaagtcaat cctggcggat tcactacccc tgcgcgaagg ccatcggtgc
     1621 cgcatcgaac ggccggttgc ggaaagtcct ccctgcgtcc gctgatggcc ggcagcagcc
     1681 cgtcgttgcc tgatggatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     1741 agtgcagccg tcttctgaaa acgacaatgg aggtggtagc cgagggtgtg gaaacacccg
     1801 actgccttgc gtggttgcgg caggcgggtt gcgacacggt gcagggtttc ctgttcgcca
     1861 ggccgatgcc ggcggcggcc ttcgtcggct tcgtcaacca atggaggaac accaccatga
     1921 acgccaatga accgagcacc agttgctgcg tgtgctgcaa ggaaatcccg ctcgatgccg
     1981 ccttcacgcc ggaaggggcc gagtacgtgg agcatttctg cgggctggag tgctatcagc
     2041 gcttccaggc gcgggccagc actgcgaccg aaaccagcgt caaaccggac gcttgtgatt
     2101 cgccgccgtc aggttgaggc ataccctaac ctgatgtcag atgccatgtg taaattgcgt
     2161 caggatagga ttgaattttg aatttattga catatctcgt tgaaggtcat agagtcttcc
     2221 ctgacatttt gcagggaatt ccatgactgg acagcgcatt gggtatatca gggtcagcac
     2281 cttcgaccag aacccggaac ggcaactgga aggcgtcaag gttgatcgcg cttttagcga
     2341 caaggcatcc ggcaaggatg tcaagcgtcc gcaactggaa gcgctgataa gcttcgcccg
     2401 caccggcg
//
LOCUS       DAPQZD010000310         2360 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPQZD010000310 DAPQZD010000000
VERSION     DAPQZD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.319"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            35..148
                     /locus_tag="RO454_004964"
     CDS             35..148
                     /locus_tag="RO454_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDY0936647.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            239..1452
                     /locus_tag="RO454_004965"
                     /pseudo
     CDS             239..1452
                     /locus_tag="RO454_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    520..636
                     /locus_tag="RO454_004965"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(2016..2249)
                     /gene="papI"
                     /locus_tag="RO454_004966"
     CDS             complement(2016..2249)
                     /gene="papI"
                     /locus_tag="RO454_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HDY0936648.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
BASE COUNT          610 a          530 c          624 g          596 t
ORIGIN      
        1 aagttaaacg agaaatcaaa aggaggtccc aaatctgaaa atgaagcatc tgcgtgatga
       61 tgttgcagcg ggacgtcgtc ggctgtatat cagagcaatc tgtccatccc tgcgtgaagc
      121 cccgttactt tattcgtacc ccttataatg gggtgttagc cagccagacc cggcatgatt
      181 actgtgaacc gccccgggaa tcctggagac taaattccct gagaaagagg taaacaggat
      241 gactaaaaat acacgttttt cccccgaagt ccgtcaacgg gcagttcgta tggttctgga
      301 aagtcaggac gaatatgact cacaatgggc ggcaatttgt tccatagctc caaagattgg
      361 ctgtacgcca gagactctgc gtgtctgggt tcgccagcat gagcgggata ccgggggcgg
      421 tgatggtggg ctcaccaccg ctgaacgtca gcgtctgaaa gagctggaac gtgaaaatcg
      481 tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc gcttattttg cgaaggcgga
      541 gttcgaccgc ctctggaaaa aataatgcca ctgctggata agctgcgtga gcagtacggg
      601 gtcggaccgg tatgcagtga acagcatatt gccccgtcaa cgtattacca ttgtcagcag
      661 cagcggcatc atcctgataa acgcagtgcc cgttgtcagc gcgatgactg gctgaagaga
      721 gagatacagc gcgtatacga tgaaaatcat caggtgtacg gtgtgcgtaa agtctggcgt
      781 cagttgttac gggaaggaat cagggtggcc agatgtacag tggcgcgcct catggcggtt
      841 atgggacttg ccggtgttct ccggggtaaa aaggtccgca ctaccgtcag ccggaaaacc
      901 gttgctgcag gtgaccgcgt aaaccgtcag ttcgtggcag aacgtcctga ccagctgtgg
      961 gtggctgatt ttacttacgt cagcacatgg cagggcttcg tctatgtggc gttcatcatt
     1021 gatgtgtttg ccggatacat cgtggggtgg tgggtctcat cgtctatgga aacgacattc
     1081 gtgctggatg cgctggagca ggcgttgtgg gcccgccgtc cgtccggcac catccatcac
     1141 agcgataaag gctctcagta tgtgtcactg gcctatatgg agcgattaaa agaagcaaaa
     1201 ctgctggcat caacagggag tacaggcgac tcgtatgaca acgcgatggc ggagagcatc
     1261 aatggtcttt acaaagcgga ggtaatacac cgtaagagct ggaaaaaccg tgcagaagtg
     1321 gaactggcca cactcacgtg ggtggactgg tataacaatc gatgattgct gggaaggctg
     1381 ggccatactc ctccggcaga agcagaaaaa gcttattatg cttccatcgg aaacaatgat
     1441 ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg ttcagtaata
     1501 tctgatcgta acggacggac gctggaatta accgatgacc gcagcacaga gagtagggga
     1561 tgcggacggt caaagatcgt ggagttggat tttataaatc tgtctccagt agtagatgaa
     1621 ctgaatacaa agttgccaga gagtggtctg tcaggcaggg aatcagaaac cggatattat
     1681 tgactggtac attgtgtcgg gctgtgtacg ttctccccgg acagacagtc tcagttcgta
     1741 gaatctataa attactgcta ctgatgctgc cggggaaagg tgtaacgaaa aagcagcctc
     1801 cgttaccgga cagcaagaag gctgaatgaa gtttatggcg tttgtatttt gtaatgtctg
     1861 gccatgcagt aaatccagac aggtttttat tatcatgtga agtattctga cataaaatac
     1921 taaatttcta ttttgtgatg aatgctgcaa aactgaatct gcactctgat gtaactttta
     1981 tctgtttcag tgaagcatgc ccacagattg agttattaag ttgtggaaga acagttctgc
     2041 cccgcctgct tctctccttt cagaagccag tatgttgcca tgcctcgcct taatggagag
     2101 cgctgaacca caccttcttt ttccagtaat aacaggtaat agcgtgcctg ataatccgtt
     2161 accgccagcg cctccgcaat ttctgcggtt ttccctccat tatgcctgtt cagaaattcc
     2221 agtatttcat tcttcatata ttcactcatc tcactgtaac aaagtttctt cgaataataa
     2281 aaatcatgct ctctgttatc aacggaaagg tatttttatt ctctgtgttt gctttatttg
     2341 tgcaatttag tgaatttgct
//
LOCUS       DAPQZD010000311         2355 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPQZD010000311 DAPQZD010000000
VERSION     DAPQZD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.320"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            131..634
                     /locus_tag="RO454_004967"
     CDS             131..634
                     /locus_tag="RO454_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0936649.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(680..1096)
                     /gene="rraB"
                     /locus_tag="RO454_004968"
     CDS             complement(680..1096)
                     /gene="rraB"
                     /locus_tag="RO454_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDY0936650.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1258..2262
                     /gene="argF"
                     /locus_tag="RO454_004969"
     CDS             1258..2262
                     /gene="argF"
                     /locus_tag="RO454_004969"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDY0936651.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          605 a          553 c          583 g          614 t
ORIGIN      
        1 tgccacgtta caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga
       61 cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt
      121 gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc tgacgttgca
      181 ggataatcct gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc
      241 tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca
      301 gcctggtcat gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat
      361 tgcgccatta gcaggtagtg aatcggacat ttgcgaactg caaaagatgt attttcttcc
      421 ggctattcgc ggtaaagggc tggcgaaaaa actggcctta atggcgatgg agcaagcacg
      481 agagatgggc ttcaaacgct gctatctgga aacgaccgct tttttaaagg aagccattgc
      541 gctttatgag catttgggct ttgagcatat cgactatgcg cttggctgca cgggccatgt
      601 cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt gtgtgctatc
      661 gggcatttta atcaattaat tagtggcgaa caccgtcatc gtcttcatcg acaaaatctt
      721 catcgtcgcc gtcttcgcca ttgggatctt caaagtaagt gccccaaccg tcgtactcaa
      781 cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat
      841 tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca
      901 gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgct gctttttcca
      961 gggtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc
     1021 tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt
     1081 gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac
     1141 accactgaca ttgcctccac ctttgtgaca aagatttatg ctttagactt gcaaatgaat
     1201 aatcatccat ataaattgaa ttttaattca ttgaggcatt agccacagga gggatctatg
     1261 tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaactcaac
     1321 accttgttgc agttagccgc gaagctgaaa gccgataaga aaagcggtaa agaagaagct
     1381 aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgactcg tacccgatgc
     1441 tctttcgaag ttgccgcata tgaccagggt gctcgtgtta cttatctcgg cccaagcggc
     1501 agccagattg gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat
     1561 gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct
     1621 ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagct gctggcggat
     1681 ctcctcacca tgcaggagca tttgcccggt aaaacattca acgaaatgac gctggtctat
     1741 gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt
     1801 ctggatttgc gtctggtcgc gccacaggca tgctggccgg aagctgcgct ggttacggaa
     1861 tgccgcgctc tggcacagca aaatggcggg aatattacgc tgacggaaga tgtagccaaa
     1921 ggagtagaag gtgctgactt tatctatacc gatgtgtggg tgtcgatggg ggaagcaaaa
     1981 gagaaatggg cggaacgaat tgcattgctg cgtgattatc aggtgaataa caagatgatg
     2041 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcatt tcatgacgac
     2101 caaacgacgc ttggcaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
     2161 actgatgagg tcttcgaatc tgccgccagc attgtatttg atcaggcgga aaaccgcatg
     2221 cataccatca aagcggtgat ggtcgcgacg ctcagtaaat aattgatatc gctataaaga
     2281 tgccgggaaa tacaaaacta tcccggcatc acattatttt atgcagtgat aggtctattc
     2341 ttccacccaa ttttt
//
LOCUS       DAPQZD010000312         2264 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPQZD010000312 DAPQZD010000000
VERSION     DAPQZD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.321"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..364
                     /locus_tag="RO454_004970"
     CDS             <1..364
                     /locus_tag="RO454_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDY0936652.1"
                     /translation="KVPAAKITKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKD
                     TPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVV
                     LYDQMNRGGPAGNAVVMH"
     gene            387..1349
                     /locus_tag="RO454_004971"
     CDS             387..1349
                     /locus_tag="RO454_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDY0936653.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1390..1812
                     /locus_tag="RO454_004972"
     CDS             1390..1812
                     /locus_tag="RO454_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDY0936654.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAVL"
     gene            1870..2226
                     /locus_tag="RO454_004973"
     CDS             1870..2226
                     /locus_tag="RO454_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936655.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKSRTE"
BASE COUNT          627 a          484 c          648 g          505 t
ORIGIN      
        1 aaaagtgccg gctgcgaaaa ttaccaaagc gtatttcgag ctgggaatga cgttcccgga
       61 actgtatgac gacagccatc cggaagccct ggcccgtaat acccagaaaa ttttccgttg
      121 gctggataaa gacacccctg atgctgttga aaaaatgcag gctctgttac cggcgatcga
      181 aaaggcgatg ccgcctttgc tggtggcccg tatgcgcagc cacagttctg aatattaccg
      241 tgagatcgtc gaacggcggg atcggctggt gaaggatgtc gatgattttg ttgcgtcagc
      301 ggttgttttg tatgaccaga tgaatcgcgg cggcccggca gggaatgctg tggtgatgca
      361 ctaaaagcac ggtgttcggg ggttttatga gcagcaagct tcatggtctt gtctgggaag
      421 ggtgcgcctt caccggcatg atcttatcca gggtggcggt tatggcccgt cttgcagact
      481 acagcaatga cgagggcgtg tcatggcctg ccattgaaac tatccggcgt cagatcggtg
      541 caagaagtga atccacagtg aaatcggcta ttgcagaact ggcgaaagag ggctggctga
      601 cgaaggaaga gcgtaaggtc ggtgggcgta atgtaagcaa tatctatcgg cttaatgtgg
      661 aaaaactcga agcagctgcg gcggcggcgc gtgagtcata taaaccgaaa agaaaaatta
      721 gcccggcaaa aaatgacccg ttaacagttg acccgtcaaa tattgacccc tcaacggttg
      781 acccgtcaaa ttttgatgga tcaactgttg ataaaaaact gccgattagg gggccgatga
      841 ttgaccccga tccgtcagta ttaaaacctg atccgtcaga taaaagatct tcttgtccgg
      901 acgcttcgca accggacccg cagacggctg aacaggattt tttaacccga caccctgacg
      961 cggttgtgtt cagtgcgaaa aaacgccagt ggggaagtca ggaagatttg gtgtgcgcac
     1021 agtggatctg gggacgaatc gtgagtcttt acgagcaggc ggccagctat gatggcgaga
     1081 tcactagacc gaaagaaccc aactggacag catgggccaa tgacgttcgc acaatgcgga
     1141 tgctggatgg cagaactcac agacaaattt gtgaaatgtt tgggcgtctc cagcgggatt
     1201 cgttctgggt aaaaaacatc atgagtccgg caaaactccg ggaaaaatgg gatgaactgg
     1261 ttatccgcct ggggcgttcg cctgcgcagc gttgcgtgaa tcacatttct gaaccggaca
     1321 ctgaaattcc gccgggcttc agggggtaag tgttaatttc tggtcatgag gtaattttca
     1381 ggagggcttg tggcaaaagt ttttacacaa gaagagcggg aaaaaattaa agggcaggtt
     1441 gttgaactcg tacgccagag tgggcgcgag acgttacgac aactggaaac taaaactggg
     1501 gcaacaagat atctgatgag cgttctcgca agagagctgg ttgccagcgg cgatgtatac
     1561 aactctggtt acgggttatt cccgtctgaa caggcgcgta aggactggca aaatgcccgt
     1621 aaaaagctct caagggcaaa gccgaagaaa ccatctgcgg ttgatccgga ccttatctgg
     1681 tcattaccag acggagaaat acgccgctac gacaggcgcc tgaatataat ctgtcgcgag
     1741 tgccggaaga gcgaagctat gcagcgtgta ctggctttct atcagggtaa ttttcaggag
     1801 gcggtactgt gagtgaaatt agctatcagg cttcaattgc cgctggcatt cgcatcaaag
     1861 gagaggagca tggaaataaa accagaggat gagttaagca atatcgtttt atttccggta
     1921 aaagaggatg accctcgtaa tcaggttaat tttctttatg agccatcgga aagaccatat
     1981 tgccatcacg cttctgtccg ggttgacgaa aaagagcgtc aggtccgctg taaaatctgc
     2041 ggtgcagttg tggagccatt tgactggatg ctctctgtgg cgaaaagaga aaccagactg
     2101 gcagatgatg taaggctctt gcgccaggag gaacgggaaa ggcgaaaaaa tatagaaaag
     2161 ctaattcaga ttgagcgtaa cgcgaaagcg cggatacgca gggtgacaaa atccagaact
     2221 gaataattaa atttagcact gttaaaaatt taatccttaa ccgg
//
LOCUS       DAPQZD010000313         2252 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPQZD010000313 DAPQZD010000000
VERSION     DAPQZD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.322"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1388..1717
                     /locus_tag="RO454_004974"
     CDS             1388..1717
                     /locus_tag="RO454_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936656.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFA"
     gene            1872..2183
                     /locus_tag="RO454_004975"
     CDS             1872..2183
                     /locus_tag="RO454_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936657.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWVAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
BASE COUNT          697 a          552 c          484 g          519 t
ORIGIN      
        1 cacccgattt ggtctaaaaa attgacgttt tcacacatca ctgcttatac ccgtaattct
       61 acgcagcaga cagcgccaca gcgtccaatt ttcaccacag acgacccaac acactaccaa
      121 caaaaacaca cgctcaaaat cgttcccgtt gcgttacata accatataaa caacttattt
      181 tcaggcataa caaaacaact aaataacaca tatgccattc ccaaaacaac caacctcttt
      241 atggcaggct accgaaaaga cccacctctt cttcccaggc taccggacaa ccaacctaac
      301 tttcacaggc aatccgaaaa cattcatacg cgcgataccc acgacacgcc attacccaaa
      361 cagcgaagaa acatcagaat cactgaaacc ccaacgaagc ctgtaaccgc aaggtttctc
      421 tcaatcccaa aagacacgaa acataacaat cacgggaaac gccattaccc aatacacgaa
      481 taactacaaa agcccttcac gcactccaga caaacaactc aaacacaata acgaaaggaa
      541 tcgcccacct gccattactc catacacgga gaagaacaat caccaaaacg acgagaacac
      601 cccatagaag aaccaccgca aaacgaacga aaccatttac accgacagag agaacaacac
      661 gcccaattca ccaacagaaa tagtcgccgt atagaacgtt ctggaaggag tgagcattaa
      721 cgcctataga gaggttgaga cagataaaac acgaagaaaa cacaacatgt atagcgaaga
      781 agccaggtat gtataagtgg ggagggaagg aggggtgtcg cctccttttt cgtatttatt
      841 caactcctga ttttatcccc cgtaacaccc ctacggtcag ccttcggtcc atacagggaa
      901 aaggctgcat cccgctacag aaacggctgg gttgccttcg gggaacggct ggggattttc
      961 agggaaacgg tcgagttgcc tgtgtggatt tcgggaaaag gctcgatgcc ggttcaggaa
     1021 cggcggcgta ccggtatagc gagagggaga gttgcggcta cccacctgta atgtgcggga
     1081 agaagagagg cgctcacgtg caggcgaact tacttaccgc tggattccct ctgaatgccc
     1141 agctacagcg taattgtgcc agacagccaa cgggtcacaa tcgatttcag taggttttcg
     1201 gggagatgat tattctgccc accagccacc ttcggctact aaatcagaat aatgtaaaaa
     1261 gtgtactcgg tactgaacct gaaaactcac tgccaaaacc acattcaccg gaataaaatc
     1321 ctttttcgcc tatgcgttat tgcgataata acaccaataa gaaaacatgt tgtttaagga
     1381 ttgctttatg tttgcaaata tcgacatcaa ccaaatcaag aaattaactc aaaaagagtt
     1441 tgatcagttt tatgagttag aaggttggtc ttccactctg atcaattcgc ggtgggtgct
     1501 tgagctgatg actcgtgatg acgcacctgc tttgatgatt tgcgacatgg gtgaagatgc
     1561 tgactttatg gatatgagcg aattttgtgt ggacacatac aaccgcagcc agaagtacta
     1621 cttcacatgc gatagcgaaa atgacgtgat ttctaaggtc tatcttcacc tcgtccagca
     1681 ttgggacgtt caggagtttc ttgaggtatt cgcgtaatca caatcaaagc cagcattgct
     1741 ggcttaactt aatctgtagc cgcagggaag aacctgcata tcgctcatgc gcctgatcaa
     1801 tatgctttgt agccgttttc ttactggtat tatttacgcc attgagaaaa caagttgttt
     1861 acggagttgt gatgaaaaaa tcattggttc ttggtctgga caaagaccag aagagaaaag
     1921 agaagcctgc gctggttgct caattaactt tgctggacat cgttgccaat ggaacctcta
     1981 ttcgtctgtt ccgtgaaaca gcggtgtctt tcgataaaaa cacctttact cgttatgtaa
     2041 tgaatgttcg tcgccagcgt ggaaaaggct gggtggcatt tcaaagaatg tggccggaac
     2101 atcagctcga actggctttg atggaagtta accgcgtggc ccagcaagag attcagagag
     2161 catcagtgat ggcaatagcc tgataatgtg caagtggtaa ttagtcgacc gtacgacagc
     2221 cccgccatcc ttacggggct ttttgtattg ta
//
LOCUS       DAPQZD010000314         2235 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPQZD010000314 DAPQZD010000000
VERSION     DAPQZD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.323"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(390..1862)
                     /locus_tag="RO454_004976"
                     /pseudo
     CDS             complement(390..1862)
                     /locus_tag="RO454_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(2014..>2235)
                     /locus_tag="RO454_004977"
     CDS             complement(2014..>2235)
                     /locus_tag="RO454_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDY0936658.1"
                     /translation="FSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTE
                     SVTIDLAYEGSGSGDWRTDAFIVGIGYRF"
BASE COUNT          392 a          649 c          614 g          580 t
ORIGIN      
        1 gcaacggtcg atttcgcaag aatatcccgc ccaacttttg tcagggttgc cagactggca
       61 acatcattcc ccgtaaaata cggaaacctg tctgccgcag tagcaagccc ggccagcgcc
      121 gtcagggtgg catctttcgg ttgcttaccc gcaagcgcgc tagtcatggt ggtcgcaaaa
      181 ttcgggtcgt tgcccaacgc cgcagccagc tcgttcagcg tattcagtgc gtcaggcgac
      241 gagtccacga gggcagcgat cgcagccata acataagccg tgcttgcgat ttgggtatta
      301 ttcgttccct gtcgcgcagt tggtgttgtt ggcgttccgg tcagtgcagg actatttaag
      361 ggcgctttct tgttcgtttc acccattacc gccttaaccg cttttggcgt tgccgccagt
      421 gactcggaag tgctgttggt cgcactgctg agctgtacta tccccttttt cgtcgtgctc
      481 gcatcctcaa gcgccacggc ggatgcaata tcctctgccc gttttgccgc tgtctcggcg
      541 cgcgttgccg cggattccgc cgtacttttg ctctgagctg ccgccgtcgc actgccagct
      601 gcctctgtcg ccttcgtgga tgccgtcgtg gcgctgccct ttgctgctga cgcctgtctg
      661 gtcgcctcat cttttgaagc agacgcagat gatgccgatg acgccgctga actggctgac
      721 gatgcggctg ccgccttaga ggaagcagca ttgtctgctg aagtctttgc atttgtttca
      781 gaggtttttg ctgcagaagc agacctcgct gctgcagtgg cttgctcagt ggctttgcca
      841 gccttcgttg tggctgttga agcggatgat gcggcgcttt ctgccgattt tccggcggcg
      901 gtggcactgg ctgaggcctg cccggcactt gttgacgcgg cactggcaga taatgcagcc
      961 gctgtttttg aacctgccgc agctgaggcg ctctgtcccg ctgctgtttc agaagactta
     1021 gcgttcgtct cggacgtttt tgccgccttc gcggaatttc ctgccgccgt tgccgaggaa
     1081 gctgcactac tggcacttga tgatgcattc gtttctgaag atttcgctgc ctcttttgag
     1141 gccgccgcac cccgtgccga ggtggcagct tctgacgcct tcgtggtcgc tgtggatgca
     1201 gaagtggctg ccgatttttg tgatgctgcg gcattcgttt ctgacgtttt cgcggcactg
     1261 gcgctggtag ctgccgcgct ttttgatgac tctgcagcag cagcactttt tgctgcttca
     1321 cgggcctttg tcgatgccgt tcctgcgctg gaagacgctg actgagccga cgacgcggcc
     1381 tgtccggctg acgtgctggc ggcacgtgat gaggctgcag catcggttgc atgagttgcc
     1441 gcctcgctgg ctgatgcact ggcatcgctg gctgattttt tcgcggctgc cgtattctgt
     1501 gcaaccgcgg aggcgttacg tgacacctct tctaccatct gctcaaagcg gcgcagtgcc
     1561 tccggtcgta catcatcctc cgtcatggca ccgagaaaat cattcagcgt acctggtctg
     1621 gaaccttcat agacggtaat ggtcccggca tgtgaaggcg gaaaaccttc aaccagcagg
     1681 gtgacgctgt actgaccatg ctcaacatcc atgctgtaac gtccggcttc atccggattt
     1741 tcagaggcca ccgtgttcac caccaccgtg ctgctggttc gtctggcctt cagcacaatg
     1801 gtgcagttct gtactggttt tcctgtgcca tctttaagca cgccagaaat ttttactgtc
     1861 atacttttcc accaataaaa aaagcccgca gcagtgacgc cacgggcttc aggacagtgt
     1921 aactttacgt ttcctcaaac gcagttcacc ccataaggtg gatgaacctg cgtatcataa
     1981 caatatttac agaagataaa tcggcgtctg ttgtcagaaa cggtatccga taccaacaat
     2041 aaatgcatcc gttcgccagt cgccactacc ggaaccttca taagcaaggt caatggtcac
     2101 ggattcggtc gggttaaact gcacgccagc cccccacgcc agagacgtgt tgctgtggcg
     2161 accgtcatca cttccggtca gcacatcgtg cgttttcccc ttgttgtcag ttacgcggag
     2221 ataatccccg gagaa
//
LOCUS       DAPQZD010000315         2228 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPQZD010000315 DAPQZD010000000
VERSION     DAPQZD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.324"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..141
                     /locus_tag="RO454_004978"
     CDS             <1..141
                     /locus_tag="RO454_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936659.1"
                     /translation="PVKNIIPIITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIP
                     DN"
     gene            143..487
                     /locus_tag="RO454_004979"
     CDS             143..487
                     /locus_tag="RO454_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDY0936660.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            491..826
                     /locus_tag="RO454_004980"
     CDS             491..826
                     /locus_tag="RO454_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDY0936661.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(885..1997)
                     /locus_tag="RO454_004981"
                     /pseudo
     CDS             complement(885..1997)
                     /locus_tag="RO454_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582666.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          599 a          512 c          483 g          634 t
ORIGIN      
        1 cccgtaaaaa acataattcc tatcatcaca gtcacgctca ttttgaatta tctgaataat
       61 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
      121 tacttaatac cggacaacta agttgaagaa aaaaatactc gccttcgggc ttatctcagc
      181 attgttctgc tccacgccag caatggcgga tatgaatcgc accacgaaag gtgcattgct
      241 aggtgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
      301 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgt gacggtaaaa atgcgcgtaa
      361 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
      421 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
      481 gaaataagga atgatgatga aaaaatttgc tttactggcg ggtctgtttg ttttcgcacc
      541 aatgacctgg gcgcaggact acaatattaa gaacggtttg ccatcagaaa cctatattac
      601 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
      661 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
      721 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagatccgc aaatgctgtt
      781 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtta
      841 ccgcgtcgat gaattcgacg cggtttttta gctaacgggc gggatcagaa cttatatttc
      901 aggcccacca tcattgcggt gtcattgtag ccattatcac ccagctgcac gcccacatta
      961 ccccagacgc tggccgccgg attcagattg ccatttacgc catgtttcat ttcccccaga
     1021 ttacgagccc cgtcttgttc gactttcaca ccgttcattg agacagcaaa atctttactg
     1081 ttatggatcc cgttcatttc aacaaaaggt tcaaagtcgt caccgtgcgg accgctgttt
     1141 ttctcctgag tacgaataaa ggtgcggaaa ccaagtcggc tctggatgtt gtaatttgcg
     1201 tcattctcaa tacgtgagcc attggcttcc gtgtgcgcat tatgatcgac gcccatccag
     1261 atggcctgtg ccgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg
     1321 ccaatttgac atagccaaag ttggcttgaa cgctcctgtt cagttacagg atcgatatag
     1381 cgcgtttcac ccgcacgatc gttcagacgc gtaacaaaaa gagtgttagc ggctgccagg
     1441 ttggcgacgt aactgccagc gtcgggtcgc aggacggggg taggcgtttc tgttgattct
     1501 gaagtgacat tggaaaaaaa tcactctcta ccattgtata tttttgcaat gggggcttta
     1561 gtgccatttg ttgtaataga acttttttat atcaatgtta ccttgtcgaa tagcaactgc
     1621 ataacttcca tcagccacag tttcaagtac cacataatct aaattaatat atgctttatt
     1681 cccattagca taagtaccat agctattaat accattagtt gttatggaac caccattcat
     1741 ttttaaatca gcacggctaa taacttcgta actgttagaa tcacaaactt catcatctac
     1801 agcatcgaca catgttcgag actctccagg acttgaacat gcaagaactc ctactgcatt
     1861 ttccccatag gtaacaatgt tcgttttatc tgtggtttca agactggtgt tattttccac
     1921 agtcccagaa tcattaacct gacttaaaaa tcccgctgaa tatgccccct cgtctcgact
     1981 gaattatcac cattcataac gatggtgcca ccaaattcac tgactactgc gctggctgtt
     2041 atccctccat tttctttgtc agatacgctt ccagaggtct taatattgct atcgcttata
     2101 tataatactt tgttttggct atcaacagaa ataccataag atccatcacc actagtcatc
     2161 acactggcat tatttatata tccagttaca ccgtcatcca ctttcacagc atggctttcg
     2221 tcttaagt
//
LOCUS       DAPQZD010000316         2163 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPQZD010000316 DAPQZD010000000
VERSION     DAPQZD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.325"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..255)
                     /locus_tag="RO454_004982"
     CDS             complement(<1..255)
                     /locus_tag="RO454_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDY0936662.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSSMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQ"
     gene            complement(258..2039)
                     /gene="hcp"
                     /locus_tag="RO454_004983"
     CDS             complement(258..2039)
                     /gene="hcp"
                     /locus_tag="RO454_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDY0936663.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKVKGCTDAGTAEEPAENFGQVA
                     IFVQPVVDDCCGYHHPEANVIGEPAEAQTMLLFPSSVLATQIWGKWSLSGVLSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEF
                     REALWLEVSQEPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIAI
                     EYGGGVYDIDNLRIVTPRLHDEIHYRR"
BASE COUNT          574 a          529 c          463 g          597 t
ORIGIN      
        1 ctgtgaacgc cgccattctt tgacaatttt caggatgttt tcaggcttat catctccttg
       61 acttttaggg tagaaaatta gatcgctccc tgacggatgc tgtgtaattt caataaaatg
      121 atctactaat gaatcccagt acatttctag ctccttacct tttaatggct ttccatcttt
      181 catagatgat ctcaatccac ctaaaaaatt aagaaattcc tcctcggtat aatcttcgag
      241 cttttcttta aacatattta tctcctgtag tgaatctcat cgtgtagtct gggggtaaca
      301 attcgtaaat tatcaatatc gtatacgccg ccgccatatt caatagcaat aaaatgatgt
      361 atctgaaatt ttttaagtgt ttctttagga ccaatatagt gtccttcgtc gggggcaaaa
      421 ggtgtaaagc cctgactaat acgtaactga ttaatttccg aaaactgcgc tatcaactcc
      481 ggttcctgac tcacctccag ccacagcgcc tcgcgaaact catcaaaggt tttaaactcc
      541 cgtccgcgca atttatcaac aatatgcgcc gggattggtg cgccgttgcc gttattggat
      601 accgacatat ccagccagcg agttcctggt gaaagcggga cgccgtgccc tgttacaaca
      661 ccaggcagct ttcgcggatc ctccttcagg taaacataga taggcttaat accggatccc
      721 gccggaaaaa ccagaatata atcgttgaat tctttcggat caggaaccgg gaatggcggc
      781 gtggtatagg tatcggtgcc gtccggaatc ggcgtcacca gaatggtggg ctgctccagc
      841 ggcggcctgt cgttaccggt atggcccgga acattcccat gttccggatt ttccggcgtc
      901 caggtaatca gggggccatc gacatcgtga gcgggggtaa attcataacg ctgcgtctgc
      961 tcgttccact gcatgttcgc cacgcggacg ttttcatacg gcgtaccttc acccgtatga
     1021 acaccataca cctgaggctt gccgtgaatg tcagttcccc agaaaaaacg cacccgggtg
     1081 gtggcggtac tgcctgctag tgctttctgc cgcagttctt cctcatacca gaactcatca
     1141 cgtccaggca cacgagcact gccctcaccc gcagagggaa tatacagagc agatgccaac
     1201 gcaccgatgt aagagccgcg ggttgcactg agtacgccac tgagcgacca ttttccccat
     1261 atttgagtcg ccaacacact actcgggaac agtagcatgg tttgagcttc tgcgggttcc
     1321 ccgataacgt tagcctcggg atggtggtat ccacagcagt catcaaccac tggctgaacg
     1381 aaaatcgcta cttgaccgaa gttttcagcg ggttcttctg ccgttccggc atctgtacat
     1441 cccttcacct tcagacagga tttggcatag accgggttaa ccgctggtga agagagtgga
     1501 aggttaacaa ctggtagcgg ttcaggctta agattactgg caggtggact tttaggcgga
     1561 ataaataagc tattatagcc agcaggaaag gccaaccagt caaattccgt attggctatg
     1621 agatgtttac ataatatgta gtcataatcg acagttacac attcggtagg aagattattg
     1681 tcagaaatgt ttacatgaat agcagttaat gaagcatttc ttagctgaat ataataatac
     1741 cgttcccatc gcccgctttt atttatacga tacaaatcga tttcaataaa taaccgctca
     1801 ttctgattga tggcattaga cagtagcggt gagcttttat caatgagttt gcaaaattgt
     1861 agcccctgta gattaacgcc attaccagta ctggtaatgg cgttggtgag tgcaaaggca
     1921 aaaatttcat cttcatggcc aacctgccag cgattaccaa ccgacgcata cgtcccacag
     1981 cctgaagaaa tatcgccctg cttctcaccg attattctca ggtaaacaat atcgcccact
     2041 ttatttcatt cccgtttatc cttaacttat ttgcattaaa tagaaaactt atttctcgat
     2101 caataagaaa cgcttttaat gaaaaattat ttcaattact taacgttttc acttagctcg
     2161 caa
//
LOCUS       DAPQZD010000317         2105 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPQZD010000317 DAPQZD010000000
VERSION     DAPQZD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.326"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            67..450
                     /locus_tag="RO454_004984"
     CDS             67..450
                     /locus_tag="RO454_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936664.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(514..957)
                     /locus_tag="RO454_004985"
     CDS             complement(514..957)
                     /locus_tag="RO454_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDY0936665.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="RO454_004986"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="RO454_004986"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDY0936666.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          537 a          553 c          472 g          543 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acagtggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaatccaa tcggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggcggtaca ggccgcgcta
     1561 aatatcctct acggcattcc taagcaaact cgcaccgaca aactctctgg cgtttatgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcct
//
LOCUS       DAPQZD010000318         2069 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPQZD010000318 DAPQZD010000000
VERSION     DAPQZD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.327"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            2..226
                     /locus_tag="RO454_004987"
     CDS             2..226
                     /locus_tag="RO454_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDY0936667.1"
                     /translation="MGPQMITYTTRDGDRLDQICLAVYGRASKTTETVLYQVSNYGVT
                     DMCAVFRAGEKIVLPDIEPEPVKKETQLWD"
     gene            230..1274
                     /locus_tag="RO454_004988"
                     /pseudo
     CDS             230..1274
                     /locus_tag="RO454_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
     gene            1441..1860
                     /locus_tag="RO454_004989"
     CDS             1441..1860
                     /locus_tag="RO454_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936668.1"
                     /translation="MVVKNGLACGIMLALVGCATKPVSNEQATPVPEKQIISTALLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
BASE COUNT          575 a          431 c          552 g          511 t
ORIGIN      
        1 aatggggccc caaatgatta catacactac acgggacggt gaccgtctcg atcagatctg
       61 ccttgcggtc tacgggagag cgtcaaaaac gacagagact gttttatatc aggtttcgaa
      121 ttacggcgta acggatatgt gtgccgtgtt tcgtgctgga gaaaaaattg ttttaccaga
      181 tatcgaacca gagccggtta aaaaagaaac gcaattatgg gattgatgaa tgagtgatta
      241 cgtaaatact ggcgtggagg cgtggaaacc taatttttat atatctgctg ataataaaaa
      301 catcacggat aaaataagaa aaggtttgat caatatcacg ctgacagatt acggcggctc
      361 cagtaaacag acagacgaac tccgcgtagc aatagtttct gaaactctga aaattccggc
      421 ccgcggagta aaaatcagta ttgggctcgg tttcggtaat cagataattg ataaagggat
      481 atacatcgtt gacggtgcta gcagtggcgg tgaaccccgg gtagttgatt tcacagcaaa
      541 agcggctccg atgaacgcag caaaaggatc tccgacagtt cagagcaaaa agactcgttc
      601 ctggagcgac gtcactgtca gcgatatcgt tgcgacaata gcgagcgata atgggctaaa
      661 accacgcgta tcaaagcggt ttgcagacaa tgttatcacg caactcgatc aggtcggcga
      721 gtcagatatg cacctgatgt cgcggctcgc aacgagattc gatgcggtga gtaaacccgc
      781 gggcgggtac tggatattcc tgccgcgcgg cgcgggcgaa tctgttagcg gtgctccgct
      841 ccagcactac acgctaactc gtcgtgataa ctccagctgg ggctattcga gaaatggaca
      901 aagtggcgat agtggtagcg gtggcgaaaa tccggaaccg acgtatctga ttaaatatca
      961 cgacacagca actgggcaga tcaaagaatt gcggacaagg tagtggcggc gatcctgtca
     1021 ttgaatggcc ggcagttgag ccgtcgctcg acgcagcgaa agaagctgca ccagggctca
     1081 agggcggtgc agctaaaaaa gagttctcta tgacgtacac gacgccagcg acgctcgatt
     1141 tagtgtcgct gacagcagaa tgcaaagtca caactcaagg tttcgggacc gaggaggacc
     1201 gggactggac tatcaacaca ctgagtctga cgctcgctga aaacggtttt tcggttcggc
     1261 tgtctctgga gtaaatgggc tcggcgagcc ggtggcggta tgaatttagt attaacagat
     1321 gtcgactgca aacggcacct gcgatatttt taggcgtaag ccggagaacg aagagtctct
     1381 ggtgctgaac gttgttatcg ttagatgctg acatattttt aatattggga gttttcaaac
     1441 atggtcgtta aaaatggtct tgcctgtggg attatgcttg ctcttgtcgg ttgtgctaca
     1501 aaacctgtct caaatgaaca agccactcct gttcctgaaa aacaaattat cagtaccgcg
     1561 ttactcgaaa aacgagacgg tacgggagag gtgatcatca agcgtgactc gggtttcttt
     1621 ggaagcgcgt gtttttcccg tgtttatgtt gatggtaaag aggtggcaga tttaggtaca
     1681 gctcagaaag tcgttgtata cccaacgatt ggtgatcatg tcttcagcgc gtggccaaaa
     1741 ggagtatgtg gtggtggcat gagcgaacaa gcgggcaagg tgacaacaga tcgtactttg
     1801 atgtatcgca tagggtatgg atcaaacggt gaatatggaa ttcacccaac cgcgttttaa
     1861 tttgtgttgg ggtatgtgag tttttctcat acccctttac ctcgctttgc tcctgacagt
     1921 ttggtcacat aaaggcgcag aacatcacca tttttgccaa aaaccttcag aagacgatga
     1981 gtaagacgcg tcgtaactat atgattattt gatattcata cacaccatta tgtccggata
     2041 atttgccata attatttgaa tttattgca
//
LOCUS       DAPQZD010000319         1944 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPQZD010000319 DAPQZD010000000
VERSION     DAPQZD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.328"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(262..1944)
                     /gene="glcA"
                     /locus_tag="RO454_004990"
     CDS             complement(262..1944)
                     /gene="glcA"
                     /locus_tag="RO454_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDY0936669.1"
                     /translation="MVTRTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT          564 a          513 c          474 g          393 t
ORIGIN      
        1 atttgttggt agttttaacg attaagattg caaaagcaac agatagaacg gtgtttgctc
       61 atcaagcgaa cataattaat actgacttgt tttgcgtgat aaaaaaacag atgttctcac
      121 gcttttaatt atttagcagg ttatctgaac gcgaaacatt attgccacat ggataataaa
      181 gcgggaaata attttccatt aataagacag agtaatgtaa acgaagccgg atgatattaa
      241 cgatcatccg gctttattaa cttacgaaac taacatcccg gtaaacacat acgcctgcaa
      301 cagcgtgata atgccgatca cactggcaaa gatcagactg tgcttcacgg tgtagcggaa
      361 cagttcagat tctcggccca ccatgcccgt cgcggcgcag gccacggcga tagattgcgg
      421 ggaaatcatc ttgccagtta cgccgccgct ggtatttgct gccaccagca gagtgtcaga
      481 gacgttgatt tgctgcgccg tggtcgattg cagtgaacca aacagggcgt tagaggaggt
      541 gtccgagccg gtaaggaata cgcccagcca gccgagaaac ggtgagaaaa acgggaacat
      601 cacgcctgta cctgccagta ccagcgccag cgtggtggac atgccggaat agttggtgac
      661 gaaggcgaac gccagcacca tgccaatcga caatatcggc cacttcagac taattagcgt
      721 ttcggcaaag acgccaatcc ctttcttgat gcccacgccg aggatgaaga tagagataat
      781 cgccgcaata aaaatagcgg tgccgccagc cgagagaggg tcgaatttaa acaccgcatc
      841 cattggcgtt ggttgggcga caatgggtgc cgctttcaac acttgttgat gcaaatgagg
      901 gatctggaaa ttaatcacca gtgaataaaa cgcgccgccc ggagcaaata acgctttaaa
      961 cggcttcatg gtccagatgg tgaccagcac cgttaagatt aaaaacggtg accacgcacg
     1021 aatgatttgc cccagactat attctgaagg cacgggaccg ccagaagatg gtttattcac
     1081 caccatcgca cctgcggatt gtcccatgct gattgccgtt tcggtatttt tcggccgcca
     1141 gactttaagg aataaagcga gtgagacgat actcaccagc gccgaagtaa tatccggcag
     1201 ttccggacca atatagttag aggtaaagaa ctgcgtgaca gcgaagcttc ccccagcaac
     1261 cagcgccgct ggccacgtct ctttcacccc tttccagccg tccatcattg ctaccagcca
     1321 gaacggcaca agaaccgaca ggaacggtaa ctgacgtccc gccattgcgc caatgtggaa
     1381 cggatcgatt cccgttacct gaccggcgac cagaatcggc acgcccaacg caccaaacgc
     1441 caccggcgca gtgttggcaa tcagacacag ccccgccgcg tataacggtt tgaagcccag
     1501 gcccaccagc agcgcaccgg taatcgccac cggcgcacca aagccagccg ccccttccag
     1561 cagcgcgcca aaggagaaac caatcagtaa cacctgcaaa cgctgatcgt cagtgatgga
     1621 gataaccgag ctgcggataa tatcgaactg accgctggca acggttaatt tatagaggaa
     1681 caccgccgca acaataatcc acgcaatagg ccataatcca taaataaagc catagcccgc
     1741 agcagcaaat gccatatcaa tcggcatttt aaaggcgaat atcgcaatca gaatagataa
     1801 tataagggtt attgctccgg cgacatgtcc tttcagacgt aataccgcga gtgcaacgaa
     1861 gaagaatatt atcgggatca gggcgaccag agcggatagc cccagtcctc ccatcggcat
     1921 atacatttgg gtcctggtaa ccat
//
LOCUS       DAPQZD010000320         1932 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPQZD010000320 DAPQZD010000000
VERSION     DAPQZD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.329"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..345)
                     /locus_tag="RO454_004991"
     CDS             complement(<1..345)
                     /locus_tag="RO454_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HDY0936670.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNIL"
     gene            complement(345..1925)
                     /locus_tag="RO454_004992"
     CDS             complement(345..1925)
                     /locus_tag="RO454_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDY0936671.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
BASE COUNT          600 a          462 c          376 g          494 t
ORIGIN      
        1 gagaatatta tcaccatgcg atgtcaataa ctctattgct tttgcaatag agtacatcac
       61 actggggcta taggttatcc attcaggttt aatatctgtt tgatattgtt gataatacca
      121 gtttactatc gcattcagat attcaccatg ttgccaacga ctgtacccta aaatcgggtg
      181 ttccaggcgt ttttttaatg ctctggtgat agcgtctggt atcggaagat ccatatctga
      241 aatagagaat ggtagtacat cttttacacc aaatctgtcc tcgcaaaagt cccattgggt
      301 acaatatgtt cctttgcggg aaacaggagt attaaaaaca tccatcagta tatccccata
      361 tagttatcca tatcccgacg cagggcgatg actttagtgc cgataatgac ttgtagggta
      421 ttttgatcaa gtttaacaac cgcaatcccg ccagctattt ttattgcaac ttcgtcaatc
      481 aatcccatat ctgcgattgt taaacgtaaa cgagttacgc aattatccag tgacttaata
      541 ttctctttac caccaagtgc cttaaccatc tgctccgccg cttgcagctc tgatttatca
      601 ggagttggga tatccgctat agccgtatct tcgtgagcgt tcagcgtgac aggactggac
      661 tcttcacggc ctacggtcat caggttaaat ttaagaatgg tccaacggaa cacaaagtaa
      721 taaatgaaga aataggccac ttcgacacac accgcgatcg gccaacctgt tcttgtgcca
      781 cgcaatacgc caaagctaat aaattgaatc agatcgccag taaatcccat cttcacgccc
      841 atatatggca gaaccatgtt agcgaatcca ttcataaagg catggaagac aaacagcagt
      901 ggactgacaa ataagaacag aaactcaatc ggttcgctga cgccgccaat aatcaccgta
      961 agcaccccgg aaataagcaa tgctttgata gcctttctgt tttgagcatg agcagtgcga
     1021 tacatcgcca gcgcggcacc agccagacca tactgaatca tcagtttgcc ctgctccatt
     1081 ttccccgcca aatccagcgg aatgaccgca ttctctttga cgtacgccat aaacatgttc
     1141 agggtgccgt aatattcttc gccgccaatc acagccgaac cgccgattgg cgtaaagcgg
     1201 aaaactgacg tcaccagatg gtttaagcca aaaggaatcg tcacgcgttc ggcaactgca
     1261 tagaagaaat aaccaacagg accggaagtt gaaatccagt gtccaatgag catgaaaaga
     1321 ttgaaaaacg gcggccagat aaaaggaatg atcagaccaa atccactcat cacaatcagt
     1381 gtaatgatag gcaccagacg tgggccgcta taaaaaccta acgattcagg aatgcgtaaa
     1441 ttaacgatct ttttatacat gctggcgact aataacccag caacaattcc ccccaacacg
     1501 ctggtattgt aggactggat ccccagaatg atggtttgcc catgtgtcga catttggtca
     1561 gcaacgacca ataagtcgtg ctgtttaaga taaaagttcg ttcccagatg catcgccata
     1621 aaaccaatta agccagaaaa agcaccatag gctttatcct ctttatcttt taataatcct
     1681 aagggaatcg ctatcgcaaa caatacaggt aaattaacaa aggcaaacaa accaagacta
     1741 acaatgaaat caagtatggt tttaattatt gggatagcca gaaatggaat taactttgcc
     1801 atatcatcac tggctaaacc acttcccagc cctagcatca tgccacatac acttagcaga
     1861 gcaatgggat acataaatgc cttccccagg ctctgaaaaa aactccaggc tttcttttgt
     1921 ttcatgtggg tt
//
LOCUS       DAPQZD010000321         1900 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPQZD010000321 DAPQZD010000000
VERSION     DAPQZD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.330"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            3..815
                     /gene="nlpA"
                     /locus_tag="RO454_004993"
     CDS             3..815
                     /gene="nlpA"
                     /locus_tag="RO454_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDY0936672.1"
                     /translation="MTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQDV
                     AEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGYKL
                     VAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKEGK
                     GLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDSVF
                     IEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(819..1742)
                     /locus_tag="RO454_004994"
     CDS             complement(819..1742)
                     /locus_tag="RO454_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDY0936673.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT          582 a          423 c          469 g          426 t
ORIGIN      
        1 aactgacaac acatcatcta cgggcggggg ccgcattatt actggcagga gttctgctgg
       61 caggttgcga ccagagcagt agcgatgaaa agcatattaa agttggcgtt attaatggcg
      121 cagaacaaga tgtcgcggaa gtcgctaaaa aggtagcaaa agagaaatat ggcctcgatg
      181 ttgaactggt aggatttagc ggttcgttgc taccgaacga tgcgacaaat catggcgagc
      241 ttgatgcgaa cgtcttccag catcgaccct ttcttgaaca agataatcag gcgcatggtt
      301 ataaactggt agcggtggga aatacgtttg tcttccccat ggcgggttat tccaaaaaaa
      361 tcaaaaccgt ggcgcaaata aaagaaggcg cgacagtggc gatccccaac gacccgacta
      421 accttggtcg tgcgctttta ctgctgcaaa aagagaagct aattactctg aaagagggaa
      481 aagggttatt acctaccgcg ttggatataa ccgataatcc acgccatttg cagataatgg
      541 agctggaagg ggcacagttg ccacgtgtac tggacgaccc gaaagtggat gtagcgatta
      601 tcagcaccac ttacatccag caaacaggac tttctccggt taacgacagc gtatttattg
      661 aagataaaaa ttcgccgtat gtgaatattc tggtggcacg ggaagataat aagaatgcag
      721 aaaacgtgaa ggaatttctg caatcttatc aatcacccga agtcgctaaa gcggcagaaa
      781 ccatctttaa cggaggcgcg gtgcctggct ggtaattctc actcatgccg cgccggacga
      841 tttatttttc tggcgcggcg gcgggaatcc attgaaatca aaatcaccga tgacagaatc
      901 agcagcgttc ccagccagtc cggcaatgtg aaggtgatcc ccagcaacag caaagagagc
      961 agtgcgctac ttaatggttc tgcacagctt aaaatgctgg cttttggacc accaattaat
     1021 tgtgctcctt tcaggtacag actaaatgtc agggacgtac caatgaccac caaataaaaa
     1081 aacgccagaa tcaaactgcc gttaacgaca aagtttgtcc cttgtctggc ataaaagggc
     1141 agcaggatca gaccgccaat gagcatactc cagccgacga ccggtaacgt gccatagcga
     1201 gcaatcagcg ttgagggata ggtggtatag aatgcagcag caaatgccga ggcaatgccc
     1261 cagaataacg cggcaggaga gatcgataat gacgtcggat taccgtgtgt caccaataaa
     1321 aaagtcccga tcagcgatgt caaaatagcg caaaaaacca ggatgccggg gcgggattta
     1381 cgcaccagtg agaaccaggc gacgataata gtcggtgaga ggaattgcag caccgttgcc
     1441 gtggctgcgt tcgatttttc gatggttagc aaaaaagtga gctgtacggt tagcgcgcca
     1501 accacggaaa aaatcagtag gctaatggca tctttgtgat tgttaataat agaaaagatt
     1561 ttatcaccat gaacaaatga tagcgtcagc agaatcaatc cggcgaatat caaacgcgtc
     1621 atagtcaaaa actgcgacga catctggctt tgctccatga tgtattgcgc gcagacccct
     1681 gaacttcccc acaacacggc ggcaatcaga acgttcagca tcccctttct ggtggaaccc
     1741 atttttccct ctgcatactg ttttgtcttt tcatcaatat acgttaagga taccactacc
     1801 gatgacggtg agggagtaga gtaatcaatc agttttagtg aatggaggaa ataatgacat
     1861 ataacaatta ttcatatggt gtttaaaaaa cctcactaaa
//
LOCUS       DAPQZD010000322         1881 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPQZD010000322 DAPQZD010000000
VERSION     DAPQZD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.331"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..104
                     /locus_tag="RO454_004995"
     CDS             <1..104
                     /locus_tag="RO454_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDY0936674.1"
                     /translation="LCTLGDTPGQIFRSMVDGYHRHCTAPRGDKPTV"
     gene            complement(350..1744)
                     /locus_tag="RO454_004996"
     CDS             complement(350..1744)
                     /locus_tag="RO454_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDY0936675.1"
                     /translation="MNKLLLGLSVFLLLEYNACAAKTGGLTLEQRMALLEERLEAAEK
                     RAEKAERMLKSFDTEQHSEIRQISSEQNKKAANSYAVVESTKEKTSSPGFLLSGYNDL
                     KFYGDVEFNIDAASKPGQLVMISSGANSESVNERWDLNGRILLGFDGTRKLDNGYFAG
                     FSAQPLADMHGSVNIDDALFFFGKDDEWKVKVGRFEAYDMFPLNQDTFIEYSGNTAND
                     IYADGRGYIYMMKEGRGRSDAGGNFLISKQLDNWYFELNTLLEDGTSLYNNGNYHGRY
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVKNAYGYTDNKGNFVDQSNRSGYGIS
                     MTWNDLKTDPENGIVVTLNTAYLDASNEKDFTASINALWKRFELGYIYAHNKIDEFSG
                     VVCNNGCWIDGEGIYNIHTIHASYQFANVMDMENFNIYLGAYYSILDSNCRYSNCDIT
                     DDRYGARLRFKYFF"
BASE COUNT          554 a          448 c          329 g          550 t
ORIGIN      
        1 atctgtgcac tctgggtgac acgccggggc aaatatttcg cagtatggtt gacggttatc
       61 accgtcattg taccgctccg cgcggggata agcccacggt ctgacagtac tatttgcctc
      121 cgctgcgctg gcggagacgt caagtggcac tggtcagagt tagtctggca ttattccccg
      181 ggcagtactt cgtgacctga cgcaacttca gagccagttc ccggtagctg gctcgttgcg
      241 caataaatta aatcaaccat cgacagacag cgattattcg tatgctcacc gatggtgtac
      301 ccgaaaaacg cgttaatgta agtgtggaac aatactttgt tactatgcat caaaaaaagt
      361 atttgaaacg taatcgggca ccgtaacgat catcggtaat gtcacaatta ctatatctac
      421 agttactatc cagaattgag taataagccc cgaggtaaat attaaagttc tccatatcca
      481 tcacattagc aaactgataa gatgcatgaa tggtgtgaat gttgtatatt ccttcaccat
      541 caatccagca accattatta cacacgacac cactaaattc atcaatctta ttatgtgcgt
      601 agatataacc cagttcgaaa cgtttccaca gagcattaat actggcagtg aaatctttct
      661 cattactggc atccaaatag gcggtattaa gagtaaccac gatgccattt tccggatctg
      721 ttttcagatc gttccatgtc atgctaatac cataaccgga acgattggac tgatcgacaa
      781 aattcccctt attatcggta tagccataag cattttttac cacgtttgct tccatcgctg
      841 cggagactgt gaattcttcc gttggcgacc aggcaattac cgggcgcaga taagcgacat
      901 ttttctgctg ctccatatat cgtccatgat aattaccgtt attatataaa gatgttccgt
      961 cttcaagtaa cgtgtttaac tcaaaatacc agttatcgag ttgtttactg atgaggaaat
     1021 taccaccagc gtcagaacga ccgcgtccct ctttcatcat atagatataa ccacggccat
     1081 cagcataaat atcgttagct gtattaccgg aatactcaat gaaagtatcc tgatttagag
     1141 ggaacatatc gtaagcttca aaacgaccga ctttcacttt ccactcgtca tcttttccaa
     1201 aaaagaataa tgcatcatca atattcactg aaccgtgcat atccgccagt ggttgtgctg
     1261 aaaatccagc gaaataacca ttatcaagct tacgggtacc gtcaaaacct aatagaatac
     1321 ggccattaag gtcccaccgt tcattcactg actcactgtt cgccccggaa cttatcatta
     1381 ctaactgacc gggtttactg gcagcatcta tattaaactc cacatcacca taaaacttca
     1441 aatcattgta accagagagc agaaaacctg gagaagatgt tttttctttt gttgactcaa
     1501 ctactgcata gctgttagca gcttttttat tctgctctga actaatttga cgtatttcag
     1561 aatgttgttc tgtatcaaaa ctttttaaca ttctttctgc tttttccgcc cttttttctg
     1621 cagcttccag gcgttcttcc agcaatgcca ttctttgttc aagcgttagc ccccctgttt
     1681 ttgctgcaca cgcattgtat tccagtaata aaaaaacaga caaacccaag agtaacttat
     1741 tcattttacc cccttaaata aaaacaaatg tgggtactct tatattctct gtatcaacac
     1801 aaaataccat tcatactctt caccagactg gtatatataa tcgcaacaca ttaccaaaag
     1861 cacacagcaa agataattaa t
//
LOCUS       DAPQZD010000323         1789 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPQZD010000323 DAPQZD010000000
VERSION     DAPQZD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.332"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            52..984
                     /locus_tag="RO454_004997"
     CDS             52..984
                     /locus_tag="RO454_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDY0936676.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHRES"
     gene            complement(1026..>1789)
                     /gene="pdeL"
                     /locus_tag="RO454_004998"
     CDS             complement(1026..>1789)
                     /gene="pdeL"
                     /locus_tag="RO454_004998"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDY0936677.1"
                     /translation="PEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGM
                     IPPDQFIPLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGF
                     EKECLNLVKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYA
                     TYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQA
                     DLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG"
BASE COUNT          469 a          457 c          415 g          448 t
ORIGIN      
        1 ctacagtcat ggctggagaa attcatattc gataatggcg ggaatggttt aatgaactca
       61 atttttaccg aagagaatct gttggcattt actaccgctg cacgttttgg cagcttcagt
      121 aaagccgcag aagagttggg tttaaccact tccgccatta gctacaccat caagcgaatg
      181 gaaacagggc tggatgtggt gctgttcact cgcagtaccc gcagcattga gttaacggag
      241 tccgggcgtt attttttccg caaagctacc gacctgctga atgattttca cgccattaaa
      301 cgcagtatcg atactatttc gcagggcata gaagcgcgtg tgcgcatttg tattaatcag
      361 ctgctttata cgcccaaaca caccgcacgc ttattacagg tgctgaaaaa gcagtttcct
      421 acctgccaga ttaccgtcac caccgaagtg tataacggcg tctgggatgc gattattaat
      481 aatcaggcaa atatcgctat cggcgcaccc gacacgctgc tggacggtgg cggcattgat
      541 tacacggaga ttggcgcgat tcgctgggct tttgccatcg ctccggatca tccattagcc
      601 tttgtgccgg agccgatcgc cgaaagccag ctgcgtctct accctaatat catggtggag
      661 gacaccgcgc atacgattaa taaaaaagta ggctggttgc tgcatgggca ggaatcgatt
      721 ctggtgcccg atttcaacac caaatgtcag tgccagattt taggtgaagg aattggtttt
      781 ttgccggatt acatggtccg tgaggcgatg gcgcaatcct tgctggttac ccgacaaatc
      841 cataacccgc gccaggattc acgcatgtta ctcgcaaccc agcactctgc caccggtcag
      901 gtgacgcagt ggattaaaaa acaatttgcg ccaaatggca tattaacggg gatctatcag
      961 gatttactgc atcgggagag ttaatcgctg ccaacctccc gatgcgctgt gaatagcggt
     1021 ttacatcaac cacctgcttt cattacccat tcagagataa atttattacc cggtactggc
     1081 ggagagtaca aatagccctg taaaaagtga acgcctttgc cgatcattaa atccgcctgc
     1141 tcctgggttt ctaccccttc cgccacgata ctcaaaccag gcttacgcgc cagttcgaca
     1201 atattgtcca caatatgacc ggagatttcg tcgacactcg ccatttgcac aaacgacttg
     1261 tcaattttaa taaaatcgac cgggaacgcc tgcaagtaac gataggtcgc ataacccgta
     1321 ccaaagtcat ccagcgcaaa agtaatattg tgctgatgaa ggctgtcaaa tatcgctctg
     1381 gcttctggcg ttaccggaat aggattacgt tcagtcaact caagaaccag cttgatttta
     1441 tcgttaccta atttcttaac caggttcaga cactcttttt caaatcccgc agccaaaaaa
     1501 cagcccgccg agacgttgat accaatatgg aaattgtccg gcagcaaatg ttttaccggc
     1561 atcagaatat ccgcagtctg tttcatcagc tggcgagtca ttatgacaat aagaccggat
     1621 gactccgcca gaggaataaa ctgatccggt gggatcattc ccgtttgtgg atgttcccag
     1681 cggacaagca cttcacagcc cgtcagtacc ccagtctgcg cgcagaaaac cggttggatc
     1741 cacggtttaa actcgtggtt ttccaacgcc agactgatgg cttcaggcg
//
LOCUS       DAPQZD010000324         1734 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPQZD010000324 DAPQZD010000000
VERSION     DAPQZD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.333"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(119..1531)
                     /gene="pykF"
                     /locus_tag="RO454_004999"
     CDS             complement(119..1531)
                     /gene="pykF"
                     /locus_tag="RO454_004999"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDY0936678.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          412 a          410 c          432 g          480 t
ORIGIN      
        1 taaagtgaga ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta
       61 aataaaaaaa gcgcccatca ggacgcttcg atatacaaat taattcacaa aagcaatatt
      121 acaggacgtg aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca
      181 taactacgac atcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac
      241 ggtagaagtc atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca
      301 gtactaactg gtgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga
      361 agtatttacg tactgcgcga gcagatttac cgccctgagt agcaaccacg atcagcggag
      421 catccagttt ttcagcagtt tcaacggcac cacggcatac cgcttcggtt atacgcagtt
      481 tacggttgtc attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga
      541 tggtcgccat gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca
      601 gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag
      661 tcgggcgtgg gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac
      721 gtgcacggat acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga
      781 tttctacacc caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt
      841 cgaagttgtt gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc
      901 cgtgcgcttt caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag
      961 cagcaacaaa gtctacgcct tgttcgcaac caaagatcag gtcctgtttg tctttttcag
     1021 ccagtgctgg cagggcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt
     1081 cgccgttgtt cagcacttta cagataactt tgttaccttc aatggcggta acttccatac
     1141 cgatcagacc gtcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt
     1201 catacgttac cgcaaccatt tcgctgttgc cgataacaga tttatcagta gtgaaagtaa
     1261 aggtctgacc cgctttcaga gaaacgtcgt taccgccttc cagtttcatg gtgcggattt
     1321 ccggaccttt ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc
     1381 gcagattctg aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca
     1441 taacgttcat gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc
     1501 cgatggtgca aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga
     1561 tgggagaaac ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg
     1621 ttgtgcctcg ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt
     1681 gtctcagccc aacgtgagtg cctgtttttg agtcgttgag ttctacagtc caaa
//
LOCUS       DAPQZD010000325         1732 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPQZD010000325 DAPQZD010000000
VERSION     DAPQZD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.334"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..460
                     /locus_tag="RO454_005000"
     CDS             <1..460
                     /locus_tag="RO454_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDY0936679.1"
                     /translation="FTSLKKLRPQSVTSRIQPGSDVIVCAEMGEQWGYVGAKSRQRWL
                     FYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVI
                     SKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            732..1283
                     /gene="papX"
                     /locus_tag="RO454_005001"
     CDS             732..1283
                     /gene="papX"
                     /locus_tag="RO454_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HDY0936680.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          529 a          350 c          406 g          447 t
ORIGIN      
        1 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
       61 tgacgtcatc gtctgcgcgg aaatgggcga acagtggggc tatgtcgggg ctaaatcgcg
      121 ccagcgctgg ctgttttacg cgtatgacag gctccggaag acggttgttg cgcacgtatt
      181 cggtgaacgc acgatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
      241 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
      301 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
      361 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
      421 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttacctacaa
      481 cgtttatcga cctcacacga acaacgatgg tttaccgcct tgcttacatg aagaaaagtg
      541 gaaacaggtt attccggtgt cctgattttg tgaaccacta caataatcct ttttcacaga
      601 cttgtcagca gccagcattt atgttctttt acctgcggga atttatccgt ctgcctgtcg
      661 ggactactct gtcatacaga aatcaggtag gaataaattg tagtggaaag tcgatgttta
      721 ccggatgact gatgcgcgct tgtacacaga cagtgtgttt cagtaatatg gagaataatg
      781 aaatgaataa cacagacaca ttagaaaaaa taatcagaca ccaaaaaaac aaagaccccg
      841 catatccctt tcgggaacat ttgttgatgc agctctgtat tcgcacaaat aaaagaatgc
      901 aggataatat atctgaattt ctgggggtgt atggaataaa tcactcagca tatatggttc
      961 tcaccacatt attcgcagcg gagaaccatt gtctgtcacc ttcagagata agccagaaac
     1021 ttcagtttac cagaactaat attacccgca ttacagattt tttagaaaaa gccggatatg
     1081 taaaaaggac ggatagcagg gaggatcgcc gtgctaaaaa aatcagtctg acatctgaag
     1141 gtatgttttt tattcagagg ctcactcttg cacaaagcat gtatctgaaa gaaatctggg
     1201 attatctgac ccatgatgaa caggaactgt ttgaagtcat taataaaaag ttactggcac
     1261 atttttctga tgccagctca taaagtgcga aatatctgag gatgctggat agcttcaggc
     1321 aagagaataa tgattcttgc agatgtgttt ttccggatac aaaaacaaaa gtaaaaaatt
     1381 gcagcgccag gccacctttc aaagcaggga ggcctgtacc gcgtcgaaaa tttcagccag
     1441 ttaatgtaat tgtctgaacc aggcacttcg cccgggcagg agaaggagcg tggttgcggt
     1501 catcctgtat actgaagtcg ttatgaagga ataatacaaa taattgcccg ttttctctgt
     1561 acgatagagg caggtgtgta tgattgcatt actatgtttt tgtgatataa aaaactaaat
     1621 ggtgagatat gcattttaaa atataacaaa aatcaccatg aaataagaat aattatccac
     1681 ggagaacata aactgcagac tgttacaatg catgctgttc atcttgacaa tg
//
LOCUS       DAPQZD010000326         1685 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPQZD010000326 DAPQZD010000000
VERSION     DAPQZD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.335"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1655
                     /locus_tag="RO454_005002"
     rRNA            <1..1655
                     /locus_tag="RO454_005002"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          425 a          373 c          532 g          355 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      601 taattgatgg ggttagcggt aacgcgaagc tcttgatcga agccccggta aacggcggcc
      661 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga
      721 atggcgtaat gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg
      781 aagatgcagt gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac
      841 actgaacatt gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt
      901 ctgcatggag ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtgggcccc
      961 ttaccggggt tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag
     1021 agtaacggag gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat
     1081 ggcataagcc agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag
     1141 tgatccggtg gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat
     1201 aacaggctga taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg
     1261 ctcatcacat cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg
     1321 gtacgcgagc tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct
     1381 ggagaactga ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt
     1441 tcgggttgtc atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa
     1501 agcatctaag cacgaaactt gccccgagat gagttctccc tgactccttg agagtcctga
     1561 aggaacgttg aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag
     1621 ctaaccggta ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga
     1681 agaga
//
LOCUS       DAPQZD010000327         1678 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPQZD010000327 DAPQZD010000000
VERSION     DAPQZD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.336"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..89)
                     /locus_tag="RO454_005003"
     CDS             complement(<1..89)
                     /locus_tag="RO454_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936681.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVG"
     gene            complement(347..1600)
                     /gene="amiC"
                     /locus_tag="RO454_005004"
     CDS             complement(347..1600)
                     /gene="amiC"
                     /locus_tag="RO454_005004"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDY0936682.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT          418 a          451 c          380 g          429 t
ORIGIN      
        1 taaccgacgc caggcccaga aatgataacc tcctgtccat tcatcgatgc ctgtacacaa
       61 ttattgttca tgactttttc gatgatcatt ccatggctcc aaaaaaaaag gcaaggcacg
      121 tcagaagtaa taactacttc aaacattgcc ttgcctgatt taacagttac aagatgatta
      181 actaatgcgt atacattaca cggaattgtg cttaattaaa gagatggtcg gcttattttt
      241 caagccgaca ttcaaatatc cgacatcagt cacaaaacca gcgtctaata ttcaggtaaa
      301 tgttatctat tcgacgaaat ataaattcat tgaataaata aaaacattat ccccttctcg
      361 ccagcgtcgc cccatcggca aaatacgctt taattcccgc aagaatagac tccgcaactt
      421 cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg ataaacgccg
      481 tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaaccca gcctgttcaa
      541 cttgattttt atgcagcttg ttgattttac cgagcttatt cagcaccgct ttaccaaact
      601 taaggctgtc ggcaatggtc agcgactgca ccatatcgaa catggtgtgg tcgacatagc
      661 ggtcaccgct tttgcttacg ccaccaatca agtccgaggc gttctgggtt tgtgccagat
      721 attttgccgc agtactggtt gcgcctttgg ttgagagcgc aaacaccgag gaaccgctcg
      781 gctggcgact ggtaaaggcg tcggcatgga tagaaacaaa caagtcagcg cgctgtttct
      841 gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc gtcatgtaca
      901 ccttcatatt gccctctttc tcgatcagag agcgcagacg gcgagctatt tgcaatacca
      961 cgtctttttc gcgtgttttg tatttcccca ccgcaccgga gtcttcgcca ccgtggccag
     1021 ggtcaagcat aatgacaatc ggacgatccc gccctgcttt acccggttgt ggaccacttt
     1081 gtgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc agcgccagca
     1141 gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga cgctctttaa
     1201 acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt aattcaaaaa
     1261 ccatacgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg aacgggtcgt
     1321 cagcgcggat ttgcgcagcc atccccttga gcactgagtt caggtttaca tcttcgatat
     1381 ccaccaccac gcgttcagga ttactcaacg cgaactgctt atatttcagc tgacgatttg
     1441 attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc acgacctggc
     1501 tgaccgcagc caggctgacc tgacttacgc tcaatagcca catggcaccc gcgccttgca
     1561 gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg agcaaaagaa
     1621 atctaagctg tgtaacaagt aaacgaccaa tttgaccgga aactttagcg aaagacgc
//
LOCUS       DAPQZD010000328         1674 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPQZD010000328 DAPQZD010000000
VERSION     DAPQZD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.337"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..653)
                     /locus_tag="RO454_005005"
     CDS             complement(<1..653)
                     /locus_tag="RO454_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDY0936683.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVAIVFKNSYIIGYYENVQSGIDEYKM"
     gene            complement(650..1426)
                     /locus_tag="RO454_005006"
     CDS             complement(650..1426)
                     /locus_tag="RO454_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDY0936684.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYDAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGIGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
BASE COUNT          505 a          385 c          317 g          467 t
ORIGIN      
        1 tacattttat actcatctat accactttga acattctcat aataaccaat aatatagcta
       61 tttttaaaaa caatcgccac atcacaaaac tctttcaatg aattcaaact atgtgaaacc
      121 atcaaaaagc tagactcttt atgcctttct ttaaacaatt gagcgcactt ctctttaaac
      181 ctggcatcac cgacagctgt tacttcatcg acgatataat aatcaaactt aaatgccatg
      241 cttaaaccaa agcctaaacg cgatcgcata cctgaggagt aagttttgat cggcatatca
      301 aagtacttac cgagttcggc aaattcttcg acaaactcaa ttttctcttt cagctcttct
      361 tgcttcgcgt ataaccgcgc aacaaatttt acgttttcgc gtccggttaa acttccctgg
      421 aatccaccgg cgaggccgac tggccatgat atcgttttat tggtgatgat ctttccgcta
      481 tcggggcggt caatgccacc aatcattctc agtaacgttg atttacccgc accattacga
      541 ccaataaagg caacgctttt tcctgaaggg atttcgatgt ttaaatcctt aaacacatag
      601 tgtcgccccg ttggcgtacg atatgacttc gtcaaattct caatcttaat catgatgtca
      661 gcattgcctc ctcacgagtt cggtataacg ccagaccgat gaacagggtg accagagtga
      721 acattgcaag gtagttcaga ctcacgcctt cgctgatata gccaggcata actgcctcgc
      781 ggcttaactc tacgacatgc actaatgggt tccagagtag atatgaccag tattgcttcg
      841 gaatcgaatg tagagggaac atgatgcagg agatgaaata cagcggctta agcagtatcg
      901 gcaggacctt ttgcatttca gggaaggttt taccaacgac catgaatatt aagccgatgc
      961 cacatgaaag aatgattaac aaactccagg tgagcactag ttgtaaaaag tttgttatct
     1021 cgaaatattc acctgccatc cagacgataa gcatgagcaa tatataaaca gcatcgtaaa
     1081 tcagcgtctc aagcagtgcg cgtgcaatga tcgtatcgat gggttttact ggtcgataat
     1141 taaacaaccc ctggttcgct tcaatagcgc ctacagaacg attgctgata ctgctaaaga
     1201 taaaaaaggg aatcaggcca ttaagtaaaa acaccgggaa tgagatgtct ggcatcgtgc
     1261 ggtgcataat gtaaccaaaa atgcccaaca gtatcagcaa atgcgcagag ggttcgagaa
     1321 tcgcccacaa atatcccaga cggaacttac caaagcgtgt tcgtatttct cgtagaaata
     1381 atgcctctac ggtgactttc tggacttcaa atccacttct tgccatttga tgatgtgatc
     1441 ctaatctctt caggtacgct accgcccttg gcttaacagc taccaataca ctggaggtaa
     1501 tgcttatttg taactggata aaattcactc agacggtttg acctttttaa tagtcgcaaa
     1561 aaaatttcag cagaaattat ttccgcaggg ataaatagat ctatttccgt ctcgtttctt
     1621 ctaatccatt cttcaggcat gaatggattc ggtcttctgg taatttcaga aaac
//
LOCUS       DAPQZD010000329         1646 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPQZD010000329 DAPQZD010000000
VERSION     DAPQZD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.338"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            190..387
                     /gene="fimB"
                     /locus_tag="RO454_005007"
                     /pseudo
     CDS             190..387
                     /gene="fimB"
                     /locus_tag="RO454_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            complement(418..1638)
                     /locus_tag="RO454_005008"
     CDS             complement(418..1638)
                     /locus_tag="RO454_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HDY0936685.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISLAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
BASE COUNT          405 a          398 c          382 g          461 t
ORIGIN      
        1 tggcgtttgt atggcaaagt tattataagt aactatcgct actccattta agttcactca
       61 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattctaag
      181 ggaaaaacga tgaagaataa ggctgataac aaaaaaagga acttcctgac ccatagtgaa
      241 atcgaatcac tccttaaagc agcaaatacc gggcctcatg cagcacgtaa ttattgtctg
      301 actttgcttt gttttattca tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg
      361 gatattgatc ttaaggcaaa gtgtatatag ggtcttcccc gatcatggtg ggaagactca
      421 gtacgccata ttcagtttcc catagtggaa cataactccc agtttaaagc gttcccggtt
      481 tcggtatccc ctggccttta tcctcagcag tctgatcttg ctgttcagcg cctccgcatt
      541 tccattcgag acgcgatgac gcattgcatt cagtattccg tataaccgtt ttttaatggt
      601 tttcgctgcg tttttcatca tcggcacatc acattcagac gccagcgata tccactgcaa
      661 ccagtcattc ctgcgttctg tgctccaggg gcgatcccag atattttttg ccagctcttt
      721 cagtgtccag cactggcttg tctgttgcat ctgttcccgc aaccacatca gcttttcctg
      781 ccgggactca gtcatccatt tgtcgctgta ctgccacagg aagcgggtac ctttggcctg
      841 acgacgactt tcaacaggga ggtgcggatg ttcattctgg cgggttttat caaccacttc
      901 gcccagttgc ttcgccacat ggaagcgatc gaaggcgatt ttctcgaccg cattgggtaa
      961 atggatacgc gctgctctta tatagcccgc attcatgtcc atcgacagtg ttttgatggc
     1021 caacaactga ctgtcagtaa gcgaacggag atagctggca agactctctg tgccacgatc
     1081 atcggttaat gccagtgccc gcccgtcgcg atcagatacc acggttatat acctatgccc
     1141 ttttttaaag gcgacttcgt ctacattcat atgacgcact gataaaggct ttttaatccg
     1201 cgacaggcct cgcttaaccg cgcgggtcat gatgccgtca acggcattcc agctaagttt
     1261 aagttgtttt ctgaccgcat caacggtgct gattttaagc catgagagca cgaacgattc
     1321 gaacagcaga gtgtaccgac tgccggaacc tgcccacggt acaggcagag tctggcagcc
     1381 atgctccggg cacataacgc ggggaacatc ggcttctact aatgtcatga actggcaggt
     1441 atcaaggtgg cgccatttac gatgtcggtg atcgtgaaca gaacaggatt tcctgcaggt
     1501 cggacaggtt aactgagtat tttcagcaat tccgactgta acagtaacgg aacctgcatt
     1561 ttcatcgagg gtaagggatt taacctgcca tggtgcagtc aggttaagga tatgggcata
     1621 gagggatttt tcgtccatat aatttc
//
LOCUS       DAPQZD010000330         1556 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPQZD010000330 DAPQZD010000000
VERSION     DAPQZD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.339"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>1556
                     /locus_tag="RO454_005009"
     CDS             <1..>1556
                     /locus_tag="RO454_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HDY0936686.1"
                     /translation="TANTEFSYLLTPENYNRLFPPTLLPVEEKPEIPPEREIILTIGV
                     FFDGTGNNLTNTNLRMSFCQPETYGLDVQDLASFNKQCMSKQGKTGSGVQSYLNYYTN
                     IHWLNKLYHRQLVLDDDVFNIQEKIYIEGIGTENNKADSLVGMGLGNNDTGVIAKTDR
                     AISLLRETLAKIIVDLKDAKLVIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTI
                     ATAFQSVEYLGKPSGEVQFLGIFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHV
                     FQLTAMNECRYNFCLNSIKESWPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLG
                     NVPVEATSAYQKAAQQAELLWRHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVA
                     AAVTFRRTITNDWSKIALRVMYEVAKEAGVVFDAISVADKELKVPSHLKVICEKAISQ
                     GKAVLLCTAPMPFSDDELKTIGKYLHCSSNWNTVDYRLKNKVSAEVSAFKTFSFVNRP
                     DENWTRTIYD"
BASE COUNT          468 a          291 c          360 g          437 t
ORIGIN      
        1 acagccaata cagaatttag ttatttactc acacctgaaa attataatcg ccttttccca
       61 ccgacgctct tacctgtcga ggaaaaacca gaaataccgc ctgaacgaga aattatcctc
      121 accattggcg tattctttga tggcacagga aacaatttaa ccaatacgaa tttacgtatg
      181 agtttttgcc aaccagaaac ctatggtctg gatgtccagg atttggcctc gtttaataaa
      241 caatgtatga gcaagcaggg taaaacggga tccggagtac agagctattt gaattactat
      301 acgaatattc attggttgaa taagttgtat catcggcaat tagttttaga tgatgatgta
      361 tttaatattc aggaaaaaat atatattgaa ggtattggca ctgaaaataa taaagcagac
      421 tctctggtag gaatggggct aggaaataat gatactgggg taattgccaa aactgaccgt
      481 gcgatatcac tgcttcgtga gacactggca aagattattg ttgatttaaa ggatgctaaa
      541 ctagtaatta agcgtctaca gtttgacgta tttggcttta gtcgtggggc ggcagccgca
      601 cggcacttta ctaatcgcgt atttgaacgt gatcccgtac tgattaaaac cattgcgacg
      661 gcatttcagt cagttgaata tttaggtaaa ccttccgggg aagtgcaatt tttaggtatt
      721 tttgacacag ttgcagccat cggagggctg atggatggtt ttgaccctca tgatggtaat
      781 aactttccgg taaaagtgaa gttaccgcca ggtgtggcca aacatgtatt ccagctcaca
      841 gcaatgaacg aatgtcgcta taacttttgc ctcaacagca taaaggaaag ctggccagaa
      901 ttaagtttcc ctggatcaca tgctgatatt ggtggcggct atgacccact ggaagatgag
      961 tacctgtttt taagtcgccc ggcgattgaa acggtactgg gaaatgtgcc ggtggaagcg
     1021 acaagcgctt accaaaaagc agcacagcag gcagagttat tgtggcgcca ttcaacgctg
     1081 gctccgattc tgccttctgg tgtcctaaaa attgaatcca atattgatga aatagcgtcg
     1141 cctgaccact taggtaatgt gagaaaacgg gtcgctgcag cggtgacatt tcgcagaacg
     1201 atcaccaatg actggtcaaa gattgcgtta agagtaatgt atgaagtcgc taaagaggca
     1261 ggggtagtat ttgatgccat ttctgttgct gataaggaat taaaagttcc cagtcattta
     1321 aaggtaatat gtgaaaaagc aatttctcag ggtaaagccg ttctcttgtg tacagcgccg
     1381 atgccttttt ctgatgatga gcttaaaacc attgggaaat atttacactg ctcttccaac
     1441 tggaataccg tcgattatcg attaaaaaat aaggtgtctg ctgaagtatc agcgtttaag
     1501 acgtttagtt ttgttaatcg gccggatgaa aattggacca gaactatata cgatat
//
LOCUS       DAPQZD010000331         1554 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPQZD010000331 DAPQZD010000000
VERSION     DAPQZD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.340"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            85..642
                     /locus_tag="RO454_005010"
     CDS             85..642
                     /locus_tag="RO454_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936687.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNAMIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGFELGWSDFYTT
                     TRKPSAGDVGAYTRTECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
     gene            645..1178
                     /locus_tag="RO454_005011"
     CDS             645..1178
                     /locus_tag="RO454_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0936688.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSADGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVV
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1207..>1554)
                     /locus_tag="RO454_005012"
     CDS             complement(1207..>1554)
                     /locus_tag="RO454_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDY0936689.1"
                     /translation="AITRDASTLNPEGFSVVEVPDITANRRTDDSGKWMFKDGAVVKR
                     IYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLVNR
                     VDTANPEWPQKPE"
BASE COUNT          442 a          327 c          379 g          406 t
ORIGIN      
        1 aatcgacaaa cattcagcgg tggcctttct ggggaactgt ccggcaatgc cgctactgca
       61 acaaagctga aaacagcacg aaaaattgct ggagttggtt ttgatggttc tagcgatatt
      121 tcaattagtg ccaaaaatgt caatgcattt gcactccgac aaacaggtaa tactgttaat
      181 ggtgatacat ccgttggatg gaattgggat agtggtgcat ataacgccat gattggtggt
      241 gcatctgcat taattcttca ctttaatata aatgctggta gctgtcctgc cgtacaattc
      301 cgtgtgaatt ataaaaatgg tggcatttcc tacaggtcgg ctcgtgatgg ttatgggttt
      361 gaattaggtt ggtcagattt ctataccacg acacgaaaac cttcagcggg agatgttggt
      421 gcatatacgc ggacagaatg taactcaagg tttattacag gtattcgcct tggcggtctg
      481 tcatctgttc agacatggaa tggtcccggc tggtctgaca ggtcaggtta tgtcgttacg
      541 ggttcagtta acggaaaccg tgatgaatta attgatacaa cacaggcaag gccaattcag
      601 tattgcatta atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa
      661 acattactgc tggcaaccct aaaacaaaag agcaatacca gctaacaaag caatttaaca
      721 tcaaatggct ttattcagct gatggaaaaa actggtatga ggaacaaaag aatttccagc
      781 cagacacttt gaaaatggtc tatgaccata acggcgttat tatttgtatt gaaaaggatg
      841 tttcagcaat taatccggaa ggcgcaagcg tcgttgaatt acctgatatt acagcaaatc
      901 gccgggctga tatttcgggg aaatggatgt tcaaagatgg cgtagtggta aagcgaactt
      961 ataccgagga agagcagagg caacaagcgg aaaatgaaaa gcagagcctg ttgcaacttg
     1021 tcagggataa aactcagcta tgggactcac agctacggct gggcatcatt tccgacgaga
     1081 ataaacaaaa attaaccgag tggatgctct atgcgcagaa agtcgaatcc acagacacct
     1141 ccagcctgcc agtaacattt cccgaacaac ctgaatgaga caaggcccga taacgggcct
     1201 taatttttat tcaggctttt gtggccattc aggatttgcc gtatccacac ggttgaccag
     1261 aacactgtag cgttcccatg cttccagtcg tgtgcgttcc tcgtctgttg ccatattcag
     1321 cctgacagcg cgttccagcg gctggatgac tgattcagct tcggaaagca atgcggcctt
     1381 ttgtgattcg gcctgttgtt gctgttcgtc agccgtataa atccgtttaa ccacagctcc
     1441 gtccttaaac atccacttac cggaatcatc ggtccgacga ttggccgtta tatcgggaac
     1501 ctcgacgacg ctaaaacctt cgggattaag cgtggaggca tctctggtga tggc
//
LOCUS       DAPQZD010000332         1540 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPQZD010000332 DAPQZD010000000
VERSION     DAPQZD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.341"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..99)
                     /locus_tag="RO454_005013"
     CDS             complement(<1..99)
                     /locus_tag="RO454_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936690.1"
                     /translation="MEIKVQRPSLWMINTVFLLLPINNHQTNTIKLI"
     gene            complement(152..556)
                     /locus_tag="RO454_005014"
     CDS             complement(152..556)
                     /locus_tag="RO454_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDY0936691.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(777..1508)
                     /gene="bluR"
                     /locus_tag="RO454_005015"
     CDS             complement(777..1508)
                     /gene="bluR"
                     /locus_tag="RO454_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDY0936692.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          401 a          340 c          335 g          464 t
ORIGIN      
        1 tatcaatttg atcgtattgg tctggtgatt atttatggga agcaataaaa agacagtatt
       61 aatcatccat agggatggtc tctgcacttt tatttccatt atgctaatgc cttactgaat
      121 tatgaagcat ttcttaagct cccaacttta gctagattaa tggtttatta ttttctacat
      181 cttcaatata taaaagcgta ttatcaatgg cgtagtaact gcgtttgtta tgattaacat
      241 cagtaaccca ccggaaaacg cctgcgcctg ccagtgttga acagtattcc cgaaatgtag
      301 attttccgcg aatatgaagc aatgcggcct cttttatttt agctgggttc ttggtcgtac
      361 taacttttaa caggttcctg gttcctctta ataacaaaac cgtgtcatcg tgagtaataa
      421 ttctgatgtt atccgtagcc agataataaa tgtaatgtgc aatacggtga tgttttaatt
      481 ctgaataaaa ccaggagaag ttttgctctt ttctcacttg ctcaaacatc ttttgaaaaa
      541 caacgacctg atccatcagg ataataacct cttgttaggt gtgagactgc gtagtgtgca
      601 cgatcggttt taccacttca atctggtctg tcctttggct gtgatatgta caaagtgtca
      661 aagggggatt acctgaattc tcccggtgag cattttgcaa cggaccagct ccgctacaaa
      721 cgctgttgtg ggttcagatt ataacattct gtctaagggg cgggataaag gtgaatttag
      781 ggggcatgaa agatgacttt ataaccttgc tcaccccagt gttgtaaaag ttcgttttgc
      841 cttctcgttg gtgccatgcc tgtccagaca atcaatgttt gcgttgggaa cagttccggg
      901 cgcggcgatt caatgggatc agcaagaaca gaaatgtgcc atcctgacaa tgataaacgc
      961 catgcttcca gccacaatcg tgctctctct tcaacattcc acgccatcag aatggcctct
     1021 ttaccgggct tacggcgcat ttcgaaaagt gaggccgctg cgtattcaat taatgcgccg
     1081 tcaaacatac tgctcataat gcgggaggtg ttgtgatcaa gcacgagacg ctggcgaaca
     1141 ggaaggtaaa catgattaat caattgatct actgggtact ctcgacccag tgaaataatt
     1201 cgcgcgcgta gtttggctgg attagccata cgaagaattg acatcatctc ttcttgcagg
     1261 cggctccagt catcttgcgt atcctggcgg gtagtttcca gtaatgcttt aactttgcct
     1321 acaggaacgc cattacttat ccaacgcttg atctcttcga tgcgttgtat gtcttcttca
     1381 tcaaagagtc ggtgtccgcc ttcactgcgt tgtggtttta acaaaccgta gcggcgttgc
     1441 caggcccgga gagtgacagg attaatcccg caacgttcag caacatcacc aatgctgtaa
     1501 taagccacaa ttcctccttg cggtcataaa tctccgtcgc
//
LOCUS       DAPQZD010000333         1499 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPQZD010000333 DAPQZD010000000
VERSION     DAPQZD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.342"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            215..1426
                     /gene="bluF"
                     /locus_tag="RO454_005016"
     CDS             215..1426
                     /gene="bluF"
                     /locus_tag="RO454_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDY0936693.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          433 a          311 c          309 g          446 t
ORIGIN      
        1 ttagtgctaa ttcatataaa acaatgcatt acgataaaac atattgcatc atttaaatga
       61 caacctatac aggttttttc accaaaccgt cttcaacctt ttccactttt tatcaatgta
      121 aagaaacggt tatgttttgg tattaaaaga aagacacaag aagatatgtc tgttaccgtc
      181 ttactctcgc ctcgcccatt accctggatt gttaatgctt accaccctta tttatcgtag
      241 ccatatacgt gacgccgaac ctgtcaaaaa aatcgaagaa atggtttcga tagcaaatcg
      301 caggaacatg cggtctgacg taacagggat cttactgttt aatggttctc attttttcca
      361 gcttctggaa ggtccggaag aacaggttca aatgatatat cgggccatat gccaggatcc
      421 acggcactat aatattgttg aactgttgtg cgattacgcg cctgctcgcc gttttggcaa
      481 agcaggaatg gaattatttg atttacgcct gcacgagcga gatgacgttt tacaggccgt
      541 attcgacaaa ggcacatcaa aatttcagct aacttatgat gacagagcgc tacaattttt
      601 tcgtactttt gtccttgcaa ctgaacaatc aacctatttc gagattcctg ctgaagactc
      661 ctggcttttt atcgctgacg gatctgataa agaacttgat tcttgtaccc tttcatcaaa
      721 tataaacgac cactttgcct ttcatcctat tgtcgaccct ttatcgcggc ggataatcgc
      781 ttttgaagcc attgtgcaaa aaaatgaaga cagcccgtca gctatagcgg ttgggcagcg
      841 taaagaaggg gaaatctaca cagcggatct caaaagtaag gcgcttgcat tcgcgatggc
      901 acacgcactt aagctcggtg ataaaatgat ttcaatcaat ctattaccta tgaccctggt
      961 taacgagcct gacgcagtct cttttttact tgatgaaata aaggccaatg ctctggtgcc
     1021 tgaacaaatc atcgttgaat ttactgaaag tgaagtcata tctcggtttg atgagtttgc
     1081 cgaagcggtt aaatcgctaa aggctgccgg tatcagtgta gcaattgatc attttggcgc
     1141 aggttttgct ggtttgttac tcctgtcacg cttccagcct gacagaatta aaatcagtca
     1201 ggaattgatt accaacgttc ataaaagcgg accacggcag gcaattattc aggcgatcat
     1261 gaaatgctgt acatcacttg aaattcaagt ctctgctatg ggcgtggcaa caccagaaga
     1321 gtggatgtgg cttgaatctg caggaattga gatgtttcag ggagatctgt tttccaaagc
     1381 taaattgaat ggtatccctt cagttgcgtg gccggagaaa aaataatttt cagcacatta
     1441 tttcgcatga tttctggaaa tcatcgtgaa aatgaatacg ttatgtttta atttaatcc
//
LOCUS       DAPQZD010000334         1486 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPQZD010000334 DAPQZD010000000
VERSION     DAPQZD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.343"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(66..1472)
                     /gene="gndA"
                     /locus_tag="RO454_005017"
     CDS             complement(66..1472)
                     /gene="gndA"
                     /locus_tag="RO454_005017"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDY0936694.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQRAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          370 a          385 c          358 g          373 t
ORIGIN      
        1 ataaatggca aaaaaagccc ggcacaatat acgccgggcc tcaatttatt gttagttaag
       61 tcagattaat ccagccattc ggtatggaac acaccttctt tatcgatgcg cttataagta
      121 tgtgcaccga aatagtcacg ctgtgcctgg atcaggtttg caggcagaac agcggcacgg
      181 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtact
      241 gcataagcaa cgacatcgcg cagcgcctgc tgatagtcat cggcgatttg cttaaagtac
      301 ggagccagca gcaggttagc gatctgcgga ttttcggcgt atgcatcggt gattttctgc
      361 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
      421 agatcccagt tgtattcttc agacgcagca cgcaactgag agaagccctg agcgtaagaa
      481 acgattttgc ccagatacag cgcacgacga accttctcga taaactcagc tttgtcgcct
      541 gctcgctgcg cttgcgggcc agagagaact ttagacgcgg caacgcgctg atctttcaga
      601 gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
      661 agcgcgctct ggctggtcca tttacccgta cctttgtttg cagcttcatc cagaatcaca
      721 tcaaccaggt agttaccgtc ttcatctttt ttggtgaaga tatctttggt aatgtcaatc
      781 aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccag ttcttcgttg
      841 gtgaggttca ggccaccttt aagcagagaa taggcttcag caatcagctg catatcaccg
      901 tattcaatac cgttgtgaac catcttcaca tagtggcctg cgccatcggc accaatatag
      961 gtaacgcatg gctccccgtc ttcagccact gcggcgattt tggtcaggat cggcgcaacc
     1021 agttcatagg cttctttctg gccaccaggc ataatggaag gtcctttcag cgcgccttct
     1081 tcaccgccag aaacaccagt accgatgaag ttaaagcctt ctgcagaaag ctcacgatta
     1141 cgacgaatag tgtcctggaa gaaggtgtta ccaccatcaa tgatgatgtc acctttatcg
     1201 aggtacggct taagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
     1261 aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggaacc
     1321 agttttttgc ctggattttc ggcaatcact tcttccgttt tttcacggga acggttgaaa
     1381 atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc
     1441 ataccgacga cgccgatctg ttgctttgac attgtttact cctgtc
//
LOCUS       DAPQZD010000335         1484 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPQZD010000335 DAPQZD010000000
VERSION     DAPQZD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.344"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(21..1346)
                     /locus_tag="RO454_005018"
     CDS             complement(21..1346)
                     /locus_tag="RO454_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDY0936695.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          401 a          360 c          295 g          428 t
ORIGIN      
        1 tctggcgtct gattttgagt ttatttgact aatgaaaata gatcattgag tgattcgctc
       61 atcgacggat gagtaaatat ctgatcgcgt aatatgctat aaggcagccc ggcatccatc
      121 accattttca ctatattgat catctcgtgg gagtcaacac acagcagtga tgcccctaat
      181 atacgttggg ttttattatc aacaatcgct tttaataccc cacgagtatc attcatcact
      241 ctggcacgcg gaattgcagc tacaggcaat gtcaccacct gaatatcagc accactctct
      301 ctggcctgtt cttctgtcat accaaccctg gacagtggcg gtgtcatgaa tacggaataa
      361 ggcacatttt tccgatcatc agtactacgt ctgccttctc ccagcaactc atcacgcaca
      421 atgcggtaat catccagtga aatataagta aattgcagcc cgccggtaac atctcccatc
      481 gcccaaatat tgtctgcggt ggtatgtaat tgcttgtcaa cgacaattgc cccgcgttcg
      541 tttactgcga tacctgcatt ttctggatgt aacgaagcgg tagccggttg acgaccggaa
      601 gctattaaca gtgcatccac cgccagttgg gcgtgttcgc tatgcacttg cacttgattt
      661 tcatggtgac tgattcgctc cacatgggca ttgaggatta tatcgacgcc ctgatcgcgt
      721 aaaatcgtcg cgatattatc agcaatatcc cgatcttccc gaggcaaaaa cagcgaagct
      781 gcctctaaaa tggttacttt gctgccaaaa ttagcgaaca tagaggcgaa ctcaacgcca
      841 atatatccgc cgcccaaaat acctaaatgc ccaggcaatt cttttagatt aagtaatccg
      901 gtgctgtcat atactcctgg cgtggtggta attccaggaa ttggcggaac cacggtttgt
      961 gcaccggtat taataaaaat tttctcgcca tgaatttcca gatttccctc aggccgatga
     1021 acacgcaggc tatgattatt gataaactcc gcctggccgt cgatcacgtc gatattgggc
     1081 atatccgcaa gattatgaaa attcttatta cgtaaaaaat taaccacttc atttttacgc
     1141 tgtatggcac ggacaaaatc tgtgtgctgc tgtgcgtcat gaaccaatgt tttggttggg
     1201 atacagccga tattaataca ggtcccgcca tacattgcat ttgattgttc gatgagagcc
     1261 acacgccaac ctgcttttgc cagcgtgacg gctaatgttt ttccagcctt gccaaaacca
     1321 ataataactg cctgatattt attcatgatg atttcctgtc gaaacgcgtt catttgacat
     1381 catgactata gacgcaaaag agcaaataac atttcttcac aacgcttgat attttgtctt
     1441 atcgtctggt attttctttt tcttaatcag aatcacaaaa caaa
//
LOCUS       DAPQZD010000336         1435 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPQZD010000336 DAPQZD010000000
VERSION     DAPQZD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.345"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..139
                     /locus_tag="RO454_005019"
     CDS             <1..139
                     /locus_tag="RO454_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HDY0936696.1"
                     /translation="LCEGDEQSPEKRPVILEAQNVWITYKGKLPGRITGSLKTPPESQ
                     P"
     gene            203..736
                     /locus_tag="RO454_005020"
     CDS             203..736
                     /locus_tag="RO454_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDY0936697.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            953..1135
                     /locus_tag="RO454_005021"
     CDS             953..1135
                     /locus_tag="RO454_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HDY0936698.1"
                     /translation="MRKLKMKLCALMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPAP
                     DWQTPLNGIISPSERG"
     gene            complement(1226..>1435)
                     /locus_tag="RO454_005022"
     CDS             complement(1226..>1435)
                     /locus_tag="RO454_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDY0936699.1"
                     /translation="TAVTPKETITHHFFVSGIGQEKTVDAAKICGGAENVVKTETQQT
                     FVNGFLGFITLGIYTPLEARVYCSQ"
BASE COUNT          390 a          338 c          379 g          328 t
ORIGIN      
        1 gttatgtgaa ggggatgagc agtcgccgga aaagcgcccc gtaattttag aggcgcagaa
       61 tgtatggata acctacaaag gaaagctacc gggaagaatt actggttctc tgaagactcc
      121 tccggaatca caaccttaag tcactgaccg gaacagataa acctgtccgt gggcagaaac
      181 cgataaatcc tgataaatat ccatgaacgc aaaaatcaga tacggcctgt cggctgccgt
      241 tctggcactg attgccgtcg gtgcgcccgc gcctgatatt ctcgaccagt ttctggatga
      301 aaaagaaggt aaccacacaa cggcataccg cgatgggtcc ggcatctgga ccatctgtcg
      361 gggggccacg atggtggatg gaaaacccgt ttttcccggt atgaaactgt cgaaggaaaa
      421 atgcgaccag gtcaacgcca ttgagcgtga taaggcgctg gcatgggtgg agcgcaatat
      481 taaagtacca ctgaccgaac cacaaaaagc gggtatagcg tcattctgtc cctataacat
      541 tggccccggt aagtgtttcc cgtcgacgtt ttataagcgg ctgaatgccg gtgatcgtaa
      601 gggcgcatgc gaggcgattc gctggtggat aaaagatggt gggcgcgatt gccgcatacg
      661 ttcaaataac tgctatggac aggttattcg tcgtgaccag gaaagcgcat tagcctgttg
      721 ggggatagag cagtgagcag agtcgccgcg attatttatg ctttggttat ctgcatcatc
      781 gtctgcctgt cgtgggcggt caatcattac cgtgataacg ccatcgccta caaagaacag
      841 cgtgataaaa aagtcagtga gctgaagcag tcgatcgcca ccatcgctga catgcagcag
      901 cgtcagcgtg atgttgctgc actcgatgca aagtactcga gagaattagc caatgcgaaa
      961 gctgaaaatg aaactctgcg cgctgatgtt gccgctggtc gtcgtcggtt gcacatcaaa
     1021 gcagtctgtc agtcagtgcg tgaagccacc accgcctccg gcgtggataa tgcaaccagc
     1081 ccccgactgg cagacaccgc tgaacgggat tatttcaccc tcagagagag gctgatcact
     1141 atgcaaaaac aactggaagg aacccagaag tatattaatg agcagtgcag atagagctgc
     1201 ccatatcgat gggcaactca tgcaattatt gtgagcaata cacacgcgct tccagtggag
     1261 tataaatgcc taaagtaata aaaccgagaa atccatttac gaatgtttgc tgggtttctg
     1321 ttttaacaac attttctgcg ccgccacaaa ttttggctgc atcaacagtt ttctcctgtc
     1381 caattcccga aacgaagaaa tgatgggtga tggtttcctt tggtgttact gctgt
//
LOCUS       DAPQZD010000337         1402 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPQZD010000337 DAPQZD010000000
VERSION     DAPQZD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.346"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            1..207
                     /locus_tag="RO454_005023"
                     /pseudo
     CDS             1..207
                     /locus_tag="RO454_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            378..485
                     /locus_tag="RO454_005024"
     CDS             378..485
                     /locus_tag="RO454_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936700.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(538..1296)
                     /locus_tag="RO454_005025"
     CDS             complement(538..1296)
                     /locus_tag="RO454_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDY0936701.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          412 a          325 c          305 g          360 t
ORIGIN      
        1 aacgttgggt acttttgaaa aaaatattct tttagccaac gtcagttatt tacactgttg
       61 gccgctaaaa atcatgacaa catgtcagct gttcatatag ccattcatca taatcatcat
      121 aaaactgtag aaacatatta tgctgctata aatgtaatca gccaaagcct gagttttagt
      181 gctgatgaac taaagacgta tttataaccc gctatttatg atatttgtat atcgatacat
      241 aatttttttc ataaaatgca gaaacagtga ccaggtgtac aacgccaccc gacgactgta
      301 ataacaaaaa atcccttgat gcctgtccct tttgttacac tccgttatca cgcacaagag
      361 atatgcagga cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg
      421 tggtgctact ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg
      481 gctgagcagt gacaaacgaa aacagccaga ccgcataaca cggtccggca gataatttta
      541 catgctgtaa cccaacgata aggttgtccg acgatcggta tgttccggtg cagactctgg
      601 aggttcagag ttccatgtaa cgttgtaagc caccttcagt ccaaaatgtt cattaatagc
      661 aacatttaat gcgctttcgg agttcagcgt tgtgtcttcc gcgccaaaga ccgaaacacc
      721 ctgcgtaaat ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag
      781 cggctgggtt tcactggcat tgtcggtata tttgtcgtaa cgcacaccag ggccgaattc
      841 aaagcggaaa ctgtgtaccg ggccattcaa aaactggcga ccataacccg cggtcaacac
      901 atcgcgctct cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata
      961 gtcataatca gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg
     1021 ctcatcatta gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc
     1081 ataccaggtc atagtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc
     1141 aagatatcct gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat
     1201 gacagtaaaa acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa
     1261 cataacgatg gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa
     1321 aaagagacca tcgcggtccc ggaaactttc ttaaggataa agattagcgt ccctggaaag
     1381 gtaacgaatt ataaaaaggc gc
//
LOCUS       DAPQZD010000338         1402 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPQZD010000338 DAPQZD010000000
VERSION     DAPQZD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.347"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            52..702
                     /gene="zinT"
                     /locus_tag="RO454_005026"
     CDS             52..702
                     /gene="zinT"
                     /locus_tag="RO454_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDY0936702.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          456 a          274 c          281 g          391 t
ORIGIN      
        1 gttacaatat aacattacac atcatgtaca ttaactctgg aggaaattgt tttggcgatt
       61 cgtcttcata aactggctgt tgctttaggt gtctttattg ttagcgctcc tgccttttcg
      121 catggtcatc actcacacgg caaaccctta acagaggtcg aacaaaaagc tgctaatggt
      181 gtttttgatg ataccaatgt acaaaaccga acgctcagtg actgggatgg agtctggcaa
      241 tccgtttacc ctttactgca aagtggcaaa cttgaccccg tctttcagaa gaaagcggat
      301 gcagataaaa ccaaaacatt tgctgaaatt aaagattatt atcgcaaagg ttatgcgaca
      361 gatatcgaaa tgattggcat tgaggacggc attgtcgaat tccatagaaa taatgaaaca
      421 acatcctgta aatatgatta cgatggatac aaaatactca cctataaatc aggcaagaaa
      481 ggcgttcgtt acttattcga atgtaaagat cctgaaagca aagcccctaa atatatacaa
      541 tttagcgatc atataattgc accacgaaaa tcatctcatt ttcacatttt tatgggtaat
      601 gattcccagc aatctttatt gaatgaaatg gaaaactggc caacgtatta tccctatcag
      661 ttgagtagcg aagaagtggt cgaggagatg atgtctcatt gatttttttg cgctgacttc
      721 agttatttaa cagaacagtg cctggtagta acaacaataa tttaccaaga caaataagta
      781 agacaaactc agacacgctc atacttgagt cgtgtctttt ttattatgtc tgtagaaaaa
      841 aactgagaaa aaggcgaaca ataaatacac aactcagcca tacagactgg cagcgcaaat
      901 gtctccagga gcaagtcatc tgtcatggta aagctaacca gaaatatagg ttaattttgg
      961 tgtttaaacc ttttttactt gttagtatga tacaaaggct ttcaaaaaag ctgggaaatt
     1021 caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct
     1081 tttttggctc aattattgac cccttctcta cgcaacttca gttcccgcca gcaactttgc
     1141 ggcagtttca taggatcaat gtctaaaaga ataatggtga ccgataagaa aacgactgaa
     1201 taactgtaga ttttcgctcg aaaccttcct gtcagaccca tagcgaatca agtgctgaat
     1261 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatc ctacgattgt
     1321 tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa gtggcggaga
     1381 gagggggatt tgaacccccg gt
//
LOCUS       DAPQZD010000339         1388 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPQZD010000339 DAPQZD010000000
VERSION     DAPQZD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.348"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            6..224
                     /locus_tag="RO454_005027"
     CDS             6..224
                     /locus_tag="RO454_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDY0936703.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
     gene            complement(409..858)
                     /locus_tag="RO454_005028"
     CDS             complement(409..858)
                     /locus_tag="RO454_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936704.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKFTTYDFRKNRSRYSF"
BASE COUNT          413 a          250 c          305 g          420 t
ORIGIN      
        1 tggcaatggc gatgaatacg gtttttcttc atttatcaga agaggcaatt aaacggctga
       61 acaagcttcg gggctggcgt aaggtttcgc gctctgcaat tttacgcgaa gcggtagagc
      121 aatatctgga gcgtcagcaa tttccggtgc gtaaggcaaa aagtgacagg caaaggggtg
      181 aggtagttgg tgttgaagaa cagggtaagt ggcatgagga atgacaaaaa gagtcgtgct
      241 tattaacagc aatgttttgt cattctggaa cataaaatca acatgccaga tgcctttatt
      301 ctggcatcaa tcagattgaa tttgtgctca ctaacaggaa aaattgatca cagggaatgc
      361 ataaacttta tagatatcgc tcctgatatc atccaggata ataaactatc aaaagctgta
      421 tctggaccgg tttttacgga aatcataagt ggtaaatttt tcgttacata atattctgct
      481 atcgtattca cttttatcgt catgagattt taggtaaaca tccttatgca acccatgatt
      541 tttcacaaat aaaaccagat taagattcgg ctggtagtga ttatgtgctt catcataacc
      601 aaataaaagc tcttcccggc ggcagagaaa acaagctgtt tttcctggtg cgatttttaa
      661 tttgatgtgg ggacgtggga aattagtcca aagattgcca tcgtcatcac actggttaat
      721 gttaaagcca tagggtgcga aagaagcaac tgacgcaggg tgtacactag ttttaagctg
      781 gtttttgcgg cgaatccatt cattagcaga cagaaattcc cccgccatga tgacagcggt
      841 atgaatatta tccttcatcc atttatccgc taattctctg gaattaaatg tgggtcggtt
      901 agcggtaaat gtaagcggaa gtgagggtag aggaaacggt acaatatcac ctatatcatc
      961 atgccatata tagtttggcc attggtttcc tggttgtagg tgataacgca gggcttttga
     1021 cccaccggta taaaaatggg catctgctgg taatgtagaa cgaacaattc ccttaccctt
     1081 aaaggcaact tctcgcacaa aaaattctaa aaataatacc ggccaaggaa gagtaagttt
     1141 gttaatcata gtcgaaatcc ttcattatta tgcttattca gacagtatct ttgagcttct
     1201 ggaacgttta tccatttctt ttggccattt aaatagccaa ttgtttctta aaaaaataaa
     1261 tggttgtggt ttgttctttg caccagacac agttttataa gccatataat tatggcaata
     1321 aagccatatt tgggggatat agttcttttg tcttgccatt tcttgtgcac tccacggtgc
     1381 acgggttg
//
LOCUS       DAPQZD010000340         1361 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPQZD010000340 DAPQZD010000000
VERSION     DAPQZD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.349"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>1361
                     /locus_tag="RO454_005029"
     CDS             <1..>1361
                     /locus_tag="RO454_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDY0936705.1"
                     /translation="NKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGNKDVT
                     QIYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNARIKL
                     NVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPDNAML
                     GANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERPANGW
                     DFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPLIKFS
                     AQHKQGQSNTHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNNNIVL
                     EYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKISGNG
                     TSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVTDSGVSTADSSFTLDGD
                     SSPT"
BASE COUNT          383 a          254 c          325 g          399 t
ORIGIN      
        1 aataaaaagt taaaaaaaac tgcatctggc gcgattacat ggtcggtaat tgttacccag
       61 atattatctc cagtttctct ttctttgatc ccagcaaaca gttttgcatc atctggtaat
      121 aaagatgtta cgcaaattta tgccagcgat gagcatgcaa ataaagtggc ctcatttgca
      181 gcaagtgcag gtcagagcct ggcgaataat aatgcaagta gttttgctgt aaatacttta
      241 tcaactcagg cgacaaaaga ggtcgtcgac tggttgcaac aatatggcaa tgcgcgcatc
      301 aagcttaatg tcgatgattc tttttccttg aaggattcat cattcgactt tttatatcca
      361 tggctggata ctcaggatta tgtgttattc agtcagacat cactacatcg tactgatgac
      421 cgaaatcaga ccaatattgg tttggggatt cgtcatttca ctcctgataa cgcaatgttg
      481 ggtgcgaatg ttttctatga ttacgattta agtcgtagtc actctcgtgc aggttttggg
      541 gttgagtact ggagggatta tttcaggctt ggtgtaaata cctattttgg tctatctgac
      601 tggaagaaca gtcgtgatat tgatgattat ctggaaagac ctgcaaatgg ctgggatttt
      661 tctgctgaag gatggctacc tgcttatccg caattagggg catctattca gtttgaaaaa
      721 tattatggta aaaatgtcgg gttatttgga agcgataacc tgcaggaaaa cccttacgca
      781 gttactgggg gaatttctta tacaccagtt cctctgatta agttttctgc acagcataag
      841 caaggacaga gcaacactca cgatacaacc tttggtgttg agtttaatta tcgcccgggc
      901 gtttccttgg ctgaacagct tagcagcgat aatgttgcag ttatgcgaga agtccagaac
      961 cggcgttatg attttgttga acgaaataac aacattgttc tggagtacaa gaagaagcat
     1021 gcactgaaaa tcagtttgcc agagtctgtt cagggggagg gggaatcaat catccctgta
     1081 acactgacaa tcaacaatgc tagtggtggt atcaagtctg tacagtggaa tgatagtgca
     1141 ttcactgcgg ctggcggcaa gatcagtgga aatggcacat catggcagat cactttaccg
     1201 gcttataaaa gcgaaggtgt aaattcgtgg aatgttggag ccacagtaca ggataacaga
     1261 ggcaacgttt ccaactatgc cgtgatgaat attagcgtta ctgatagtgg tgtctcgacg
     1321 gctgattcgt cttttacact ggatggagat tcgtcgccga c
//
LOCUS       DAPQZD010000341         1348 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPQZD010000341 DAPQZD010000000
VERSION     DAPQZD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.350"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..713)
                     /locus_tag="RO454_005030"
     CDS             complement(<1..713)
                     /locus_tag="RO454_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDY0936706.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKLEENRQTLLHIVFTGDKNKLPQDRE
                     SLFLANIKSVSAMPEELKDRNTLQFAMKARLKLFWRPASLDNSDALTAWEKLKFHKEA
                     GKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPFSEQRFSLPVGAGSI
                     VEWSVITDYGSSSGKRQQK"
     gene            complement(759..1325)
                     /locus_tag="RO454_005031"
     CDS             complement(759..1325)
                     /locus_tag="RO454_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDY0936707.1"
                     /translation="MNKKLLSLLVSGSLIAVVSTSVFATDTGVVNFNGKIVADTCEIN
                     VNESASTTGTVTFADTYPADYSGDGSIGTSKDFKIELIKCDPLVDKLNLTFSGTTTDA
                     GFLRLENSEAGAGSATNVGITVTNKNGGANDVKFDGSVPDASTDVDNAGSITPTIFNY
                     TANVIQVGNNAPTAGKYASSATFEVFYR"
BASE COUNT          402 a          297 c          242 g          407 t
ORIGIN      
        1 aacttttgtt gtcgtttccc agaacttgaa ccgtagtcag ttatcaccga ccattcaaca
       61 atacttcccg ctccgacagg aagactaaac cgctgctcag aaaatggtcc taccatcatc
      121 aataatgcgc caggttcaga tttgctctcg gtgggaacaa tattttttcc cgatactgtc
      181 agatcactga atgaaatata aaaaggcgtt ggatttttta ctatgagctt ccccgcttct
      241 ttatggaact taagtttttc ccatgcagtg agagcatcac tattatccaa agaagctggc
      301 cgccaaaata atttaagccg ggctttcatt gcaaattgca aggtatttct atcttttagt
      361 tcttctggca tcgcagaaac agattttatg tttgccagaa acaaactttc cctatcctga
      421 ggaagtttgt ttttatcacc tgtaaataca atatggagta atgtttgccg attttcttca
      481 agtttataaa ctggaggcgt aatgagaaag ggggctttag agccatcaat attactcacc
      541 cagctttgca ccaaatagcg tgcatatgca tctttatttt ttaatgtgat ttgaacttca
      601 ttttgatttg aaggatagat aatacgtgta ccgccaagaa caatacttgc ctgaacttgc
      661 aatgccgtaa gaagaaacgt aaaacctaaa caaactcgag aaaaaactga cataaataac
      721 cctccgtggt caaacagtcc tttcaaacac tatgaatatt agcgataaaa tacttcgaac
      781 gtcgcagatg aggcatactt acccgcagtc ggcgcgttat taccaacctg aataacattg
      841 gcggtgtaat tgaatattgt tggagtaatg ctcccggcat tatcaacatc agtggatgca
      901 tcgggaactg aaccatcaaa tttcacatcg ttagcaccgc catttttatt tgtcaccgta
      961 atacccacat ttgttgcact gccagcccca gcttcgctat tttccagacg caggaaacct
     1021 gcatccgttg tcgttccaga aaatgtcagg tttagtttgt ccaccaacgg atcgcatttt
     1081 attaattcaa ttttaaaatc ttttgacgtt ccaatactac cgtctccaga atagtccgct
     1141 gggtaagtat ctgcaaaagt gactgtccct gttgtactgg cagactcgtt tacattaatt
     1201 tcgcaagtat cggcaacaat tttaccgtta aagttaacca ctccggtatc tgtagcaaat
     1261 acactggtac ttactacagc gatgagactt cctgacacta acagagaaag caatttttta
     1321 ttcataaaaa tcccttaatt atgaggta
//
LOCUS       DAPQZD010000342         1340 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPQZD010000342 DAPQZD010000000
VERSION     DAPQZD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.351"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            195..698
                     /locus_tag="RO454_005032"
     CDS             195..698
                     /locus_tag="RO454_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDY0936708.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(776..1027)
                     /locus_tag="RO454_005033"
     CDS             complement(776..1027)
                     /locus_tag="RO454_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDY0936709.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1142..1228)
                     /gene="azuC"
                     /locus_tag="RO454_005034"
     CDS             complement(1142..1228)
                     /gene="azuC"
                     /locus_tag="RO454_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDY0936710.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          367 a          305 c          288 g          380 t
ORIGIN      
        1 atgtaaaaat ataaacttga tgatttaagc attttcttat acccgttcag acgttattct
       61 tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat
      121 tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata
      181 taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc
      241 gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc
      301 tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc
      361 gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc
      421 ccggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc
      481 aacgttcaag cacgctggca cagttagccg atgaagcgaa agaactgaat gatgattcaa
      541 ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggcctg ttactgcaaa
      601 ccattcttga tgaagtgcgc agtgcgaaac ttgctggtat gtgtcctgtg cagaccgacc
      661 aacatgttct gaatgtcgtg tcgcatcagc tacattgatc atcatcggcg ctaatgcatt
      721 gcgccgatga agtttttgag aaaccgctgc ctcatttttt tgaagcagcg gttttttaat
      781 gggattcgcc ctgtggggta aacttgagtt caatcaacgc gatggctttt tggattgccc
      841 gcatggtgac cgggtctgcg gcggcgggat ggttagtaaa gtcgatattc ttcagctggc
      901 tggacatttt ttcgcgaact tcaacgggcg cgatcacatc gagaacatcc agaatttgtt
      961 tgataaccaa ctggcaagca accacatcag aaaccagttc ctgatcggca ttcagcggct
     1021 gggacatcgt aaactcctga tagcattttg aaagccgtta tagtagcggc ttcacatctt
     1081 cagcgatagt cacatccacc gtcatcagaa cacaaaaaaa cctgccggag caggtttttt
     1141 gttatcggaa catattgcct ggcggtacgt ctttgaacgt cttgcaatag ttattgaaca
     1201 tacttttcag gatcttgcgc agtttcatcg cggtactccg accatttgtt atacaggtgt
     1261 tattgtcttt gcgtttataa tatgacaacc atcacaaaaa tcaatcttta tgtgatgtaa
     1321 atcacataaa taccccttta
//
LOCUS       DAPQZD010000343         1337 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPQZD010000343 DAPQZD010000000
VERSION     DAPQZD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.352"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..441)
                     /locus_tag="RO454_005035"
     CDS             complement(<1..441)
                     /locus_tag="RO454_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDY0936711.1"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSAL
                     DAFQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDV"
     gene            complement(579..>1337)
                     /gene="blaOXA"
                     /locus_tag="RO454_005036"
     CDS             complement(579..>1337)
                     /gene="blaOXA"
                     /locus_tag="RO454_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HDY0936712.1"
                     /translation="ASTDISTVASPLFEGTEGCFLLYDASTNAEIAQFNKAKCATQMA
                     PDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEI
                     TQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIIN
                     HNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHK
                     YVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          340 a          278 c          281 g          438 t
ORIGIN      
        1 cacatctttt gtcaccaacg agcggctgcc tatcaccgca ccgtgcccga tcttgattcc
       61 gggcatgacc attgcctcag agccgatcca aacgtcattg ccaatgacag tattacctgc
      121 tttttggaag gcatcgagtg cgcttgagaa tgcaggttct tcctgcatat aaaagaacgg
      181 gaaagatgat gcccagtcgt accgatgccc ctgattgcca gccatgataa aggaagcccc
      241 actcccgata gagcagaaac taccgatgat caacttatca acgtcatcac ggtccggaaa
      301 cagataccgt gcgcagtcat cgaatgagtg cccatgatag tagccagagt aatagctgta
      361 ccgcccaact ttgatattgg ggttcttcac ttgctcagaa agcagcttgc ctttgaaggg
      421 gctatcaaag tagttggtca taagagatcc cgcggtctgt gactttgccg tctaacgttt
      481 gaaataaggg gcgccgagcg ccagcgaggg gagccaaaag cttgcttttg gccgtcccga
      541 cttgattgaa gggttgggcg attttgccat tagatttttt ataaatttag tgtgtttaga
      601 atggtgatcg catttttctt ggcttttatg cttgatgtta aattcgaccc caagtttcct
      661 gtaagtgcgg acacaaaaac atatttatgt cctgatttgc ttataataaa cccttcaaac
      721 catccgtttt gtaaggttct atttgctgtg aatcctgcac cagttttccc atacagtttt
      781 gtactattat ccagatcttg tagatacatg ttctctatgg tgttttctat ggctgagttt
      841 ttaactggga gattgtgatt aataatttta cgcaggaatt gaatttgttc ttctggtgaa
      901 atttttaagc tactttcgag ccatgcttct gttaatccgt tgtttctttc tttatctcca
      961 gagaagtctt gatttccata atcaaaatct ttgagataat tcttgatttt atttaatcca
     1021 attttttggg ttatttcttg cgaaacccaa acaacagaaa attgcatcca cgtctttggt
     1081 gtatgattgc tgttccagat ctccattcct ttgggggttt tatcccattt gaatatggtt
     1141 ttctgatcta ttatttccgc atcaaatgcc ataagtgata atgcgatctt gaaagttgaa
     1201 tctggtgcca tttgcgttgc acactttgct ttattgaatt gagcaatttc agcgtttgtg
     1261 gatgcatcgt aaagtaaaaa acaaccttca gttccttcaa ataatggaga tgcaacagta
     1321 gagatatctg ttgatgc
//
LOCUS       DAPQZD010000344         1299 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPQZD010000344 DAPQZD010000000
VERSION     DAPQZD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.353"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>1299
                     /locus_tag="RO454_005037"
     CDS             <1..>1299
                     /locus_tag="RO454_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDY0936713.1"
                     /translation="VRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGD
                     VVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRNTVTPGGEV
                     CVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPYIPNNAGIL
                     LVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAALQKNPKNVL
                     LAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASVPWICGDTT
                     YAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPDIAGSGYYG
                     SASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKAPEIKPSPG
                     GDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
BASE COUNT          307 a          330 c          371 g          291 t
ORIGIN      
        1 tgtcagggcg gcgtcgccgt cagatcttgc ggaaaagctg acacataaac tgaaagaggg
       61 ctggcagccg tttggtagtc cggtggccat aaccccttat accctgatgc aggcgattgc
      121 agcagaaggt gatgtggtcg tcagtggtgc aactgagccg gagtggtact acgtcatcgt
      181 actggccggg caatccaatg ccatggctta cggtgaaggg cttccgcttc cggattcata
      241 cgatgcgccc catccgcgca ttaagcaact ggcccgtcgt aacacagtga ctcccggtgg
      301 tgaagtatgc gtatttaacg acatcattcc tgctgaccat tgtctgcatg atgttcagga
      361 tatgagtacg attaaccatc cccgggctga cctgagcaaa gggcagtacg gctgtgtcgg
      421 acagggctta catattgcca aaaaactgct tccgtatatc cctaataatg cggggatcct
      481 gctggtacca tgctgtcgtg gtggttcggc attcacccag ggcacggagg ggacattcag
      541 cgagtccacg ggggccagtc aggattcggc tcgctgggga gtgggtaagc cgttatatca
      601 ggatctgctt ttccgcacga aggcagcatt gcagaaaaac ccgaaaaacg ttttgctggc
      661 gatatgctgg atgcaggggg aattcgatat gacgaatgcc agttacgccc agcagccagc
      721 agcatttctt gcaatggtac agcagttccg tgctgacctt gccgggctgg cggcgcagtg
      781 tcacggtgga agtccggcat cagtcccctg gatttgtggc gacacgacat acgcgtggaa
      841 acaagaacac ggtacgcaat atgaagtggt atatggtgca tataaaggta aagaatccca
      901 gcagatttat tttgttccct ttatgaccga tggtagcgga gttaatacac cgacaaacaa
      961 cccgtcagaa gatcctgata ttgccgggtc tggttattac ggttcggcat cccgaacgaa
     1021 caaaaactgg gtatcatcaa atcgcccgac gcatttcagc tcatgggcgc gtcgtggcat
     1081 tattcccgat cgtatggcaa ctgctattct gaacgtagcc ggtcgcacct tagccttcat
     1141 tagtggtaag gcaccggaaa tcaaaccctc gcccggcggc gacacgccat cggggccgtc
     1201 tgaagatgca tccgtacgca caatctccct gttgccgaca gccggagatg ctgctgcgca
     1261 gggctggagc attaagaatg gcggaattca gttgtcaga
//
LOCUS       DAPQZD010000345         1237 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPQZD010000345 DAPQZD010000000
VERSION     DAPQZD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.354"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            41..655
                     /gene="evgA"
                     /locus_tag="RO454_005038"
     CDS             41..655
                     /gene="evgA"
                     /locus_tag="RO454_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDY0936714.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            660..>1237
                     /locus_tag="RO454_005039"
     CDS             660..>1237
                     /locus_tag="RO454_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDY0936715.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFT"
BASE COUNT          395 a          256 c          242 g          344 t
ORIGIN      
        1 cagggagaag ggaaatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
       61 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
      121 cttagcagag ttgactgaag gcggaagcgc cgttcagcgg gtggaaacac ttaagcctga
      181 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
      241 gagaaagcgc caatatagcg gaattattat tattgtctcc gctaaaaatg accattttta
      301 cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa
      361 caatatcatt gcagctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
      421 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
      481 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgctgaaaa
      541 aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcttgatgg aaaaattaga
      601 atgcaaatca ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca
      661 tgaagttttt accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
      721 cagacggaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
      781 cactacgtct gagcaacaag gaattacgtt ggctagcgag caaaaaaaat cttgtgattg
      841 cagtacacaa gtcccaaaca gctacgttgt tgcataccga ttcgcagcaa cggattcgtg
      901 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
      961 gggaatacgc agatcatcaa aaagcaatgg acgcgctgga agacggtgaa gttgatatag
     1021 tgttatcaca tttagttgct tcgccgcctc ttaatgatga cattgctgca actaatcctc
     1081 tgataattac ctttccggcg ctggtcacta cccttcacga ttcaatgcga ccgcttacct
     1141 catcaaaacc agtaaatatt gctcgagtag caaattatcc cccagacgag gtaattcatc
     1201 aatcatttcc aaaagcaaca attatctctt ttacaaa
//
LOCUS       DAPQZD010000346         1184 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPQZD010000346 DAPQZD010000000
VERSION     DAPQZD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.355"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            142..1116
                     /gene="cysB"
                     /locus_tag="RO454_005040"
     CDS             142..1116
                     /gene="cysB"
                     /locus_tag="RO454_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDY0936716.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          305 a          273 c          279 g          327 t
ORIGIN      
        1 ttttcttcac acctatacac taaggctata aatgatatag tggttatagt tagcaccttt
       61 tttattatta aatcgtatta gtcacccgcc tggtgtggcg aaaaaacgat gttctgatgg
      121 cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc
      181 aatcataacc tgaatgtctc atcaacggcg gaaggacttt acacatcaca acccgggatc
      241 agtaaacaag tcagaatgct ggaagacgag ctgggcattc aaattttttc ccgaagcggc
      301 aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgtgaagtt
      361 ctgtcgaaag tcgatgccat aaaatcggtc gccggagagc acacctggcc ggataaaggc
      421 tcgctgtata tcgccaccac gcatactcag gcacgctatg cgttaccaaa cgttatcaaa
      481 ggctttattg agcgttatcc tcgcgtttct ttgcatatgc accagggctc gccgacacaa
      541 attgctgatg ccgtctctaa aggcaatgcc gatttcgcta tcgccacgga agcgctgcat
      601 ctgtatgaag atttagtgat gctaccgtgc taccactgga atcgggctat agtagtcact
      661 ccggatcacc cgctggcagg taaaaaagcc attaccattg aagaactggc gcaatatccg
      721 ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc
      781 gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat taaaacttac
      841 gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat
      901 cccgaccttg tgcgtgttga tgctcacgac atcttcagcc acagtacaac caaaattggt
      961 tttcgccgta gtactttttt gcgtagttat atgtatgatt tcattcagcg ttttgcaccg
     1021 catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga agaaattgag
     1081 gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct
     1141 tttatgtagc gaaggtgccg gaatatattc tcctttgtta ctta
//
LOCUS       DAPQZD010000347         1126 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPQZD010000347 DAPQZD010000000
VERSION     DAPQZD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.356"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            62..262
                     /locus_tag="RO454_005041"
     CDS             62..262
                     /locus_tag="RO454_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936717.1"
                     /translation="MIKIYLLAVATGLSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            266..1096
                     /locus_tag="RO454_005042"
     CDS             266..1096
                     /locus_tag="RO454_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDY0936718.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
BASE COUNT          321 a          244 c          271 g          290 t
ORIGIN      
        1 ctcttgaaaa tatataagca cttacttatt attttcgcat aacaaccaca taggaaaaca
       61 catgatcaaa atctatctat tggcagtagc cacaggcctt tcagtggctc tcatctacgg
      121 tttactggtt ccgtcgctga tttctaccaa gagtgattta gccgtcatgt ttggagttat
      181 cgttggtttt ggtgctcctg taatcggtct tattgctggt cgtaagttta tcaactcatt
      241 aatcaaagca aaggggaaat aagtaatgaa gaaaggttta cttgcagttg ctctggcggc
      301 tatttgcaca atgggtctta ctggctgcga tcgcgtggag cctggatacg ttggcatcaa
      361 agtaaacaaa ttaggtgaag acaaagggat tggtgaagtg gttggcgttg gtcgccaatg
      421 gacaggtctt aacaccgaac tttacgtatt cccgaccttc aaacaaatga agacctacga
      481 cgagccgttc acattccaga tgagtgacgg tactgctatt ggtcacaaaa ttggcgttgc
      541 gtatctggtt aatcgtgaca aggtaacgac ggtgttccag acctatcgca aaggcgtaga
      601 cgatatcacc gaatcagatc tgcgtcagaa aattgccgac tctctaaacc gtttggccag
      661 ccgtatgacc actgactcat ttatcgacgg tggtaaggca caattgctgg acaacgcact
      721 gaaagatatt cagaaagaga tgtctccggt tggtattgag gtactgagcc tgtcatgggt
      781 tggaaagcct gattacccaa aaactgtcat tgaatctatt aacgccaaag taacggctaa
      841 ccagcgtact ctgcaacgtc agcaggaagt tgaacaacgt aaagctgagg cgaatatgct
      901 acgtgaacag gctaatggtg aagctgatgc tatccgtgct cgtgcgcaag cagaagcaga
      961 cgccattcgt ctgcgcggtg aagctctgcg tcaaaacccg aacgttatgg aactggaagc
     1021 catcaataaa tggaatggcc agttaccgca gtacatgact caaggggcta acactccttt
     1081 cattacagtg aaataactcc cctaaaaagt tcaggcgtcc agttgg
//
LOCUS       DAPQZD010000348         1081 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPQZD010000348 DAPQZD010000000
VERSION     DAPQZD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.357"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(1..541)
                     /gene="papA"
                     /locus_tag="RO454_005043"
                     /pseudo
     CDS             complement(1..541)
                     /gene="papA"
                     /locus_tag="RO454_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
     gene            complement(749..1062)
                     /locus_tag="RO454_005044"
                     /pseudo
     CDS             complement(749..1062)
                     /locus_tag="RO454_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
BASE COUNT          317 a          280 c          186 g          298 t
ORIGIN      
        1 gttgcgactg cagaaaaagc accctcagtg acttgggcat tattggctga cgacttctta
       61 acaatcgcag tataatgcag cacattatca ccatctttca gggtattagc atcaccttct
      121 gtgccatcga aagaaacgtt tttacctgca gcctgaacta caatcgcagt acctgtcccc
      181 ccgctggtat ctaactcctc attatgacca gaaacccttg gaccactaaa agccagcttc
      241 acagtccctt tttttgctgc cgagactcct cccccccctt aaatgaagtg atatcacaat
      301 taaccaattc aatatctaaa ttcattggtt tggatacgcc tcctgcttga aggaagcttt
      361 ttgaaagctg tccaaaatca atagactgat cagctgattt ctgagaaatg ctgcatggag
      421 catcaacaac agttccgtta aaggttactc taccctgccc ctgtggagta gttagagcag
      481 catttacacc aaaagacacc actgccatag ctaccgcacc ggcaataacc gacttaatca
      541 taaataacaa cctctttttc attactgaac acaccagaga tatctggcaa tttaaaatga
      601 cccgattacc tttaataacc agatcaatac aaataatatt ctcgtatttt cagggcaggt
      661 tatttatcga cagaatactg atataacctg tctgctttct gacaagggct tcttttttta
      721 tcttccagaa actccggaat gccataattt agtcaaatgc cgatgactca tctgtataat
      781 aaggtgcaag ccttgctgca agagcattca gccgtataag tctccccagt gttgtactga
      841 aatacccatt attcatctgg tatttctcgc agacctcctt acgggagtgc ccacctacca
      901 gatagtcctt catagccaga atgaccctgt cactgtgaat agaagaaata cctatcagta
      961 aaaaaaatgc atttcagaca tagagccgag caacagcacg ctctcgcgta tattcagcaa
     1021 aaacgcattt cctgaccgac tgatgacttc atgatgcgcc atgtttcccc cttctgtcgg
     1081 g
//
LOCUS       DAPQZD010000349         1079 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPQZD010000349 DAPQZD010000000
VERSION     DAPQZD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.358"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            13..912
                     /gene="pgrR"
                     /locus_tag="RO454_005045"
     CDS             13..912
                     /gene="pgrR"
                     /locus_tag="RO454_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDY0936719.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          273 a          231 c          260 g          315 t
ORIGIN      
        1 tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt cgttgttgca
       61 gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggcgcagtc agctttaagc
      121 cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg aaccacgcgc
      181 agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc tatgttgcat
      241 gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc cgggacaata
      301 cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat gcgcacattc
      361 ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt gaccgatgtc
      421 gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg agatggataa agatatgatc
      481 gccattcgaa tcgggccaga cataccaatg gctattgttg gctcaccgga ttatttttct
      541 cgtcgaagtg ctccaacgtc agtgtcacaa ttaatagatc atcaggcaat taatttgtat
      601 cttcccacat cgggtacagc aaatcgctgg agattaatac gcggtggacg tgaagttcgt
      661 gttcgcatgg aaggtcagct tttactgaat acgatagatc tgatcattga tgctgcaatt
      721 gatgggcatg gattggcgta tctaccttat gatcaggtgg agcgggctat taaagaaaaa
      781 aaactgatac gtgttttgga taaattcaca ccagatttac ccggttatca cctgtactat
      841 ccacaccgtc gacatgctgg ctcggcattc tcattattta tagataggct gaagtataaa
      901 ggtgctgttt agcactactt gctgatacat taatttaatt cttctcttaa cgtattctca
      961 gttcctttca acgttttggt tattttttat tcttcgcaca atggcgacag atgctgatta
     1021 tgataccgaa aacgggtttg aatgtgcgaa gcccgaacgt agtgttcgga gttctatgt
//
LOCUS       DAPQZD010000350         1060 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPQZD010000350 DAPQZD010000000
VERSION     DAPQZD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.359"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            9..>1060
                     /gene="waaB"
                     /locus_tag="RO454_005046"
                     /gene_synonym="rfaB"
     CDS             9..>1060
                     /gene="waaB"
                     /locus_tag="RO454_005046"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HDY0936720.1"
                     /translation="MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDK
                     MDKGWLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKA
                     RKKSGIDVPVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIF
                     NPVEAKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGS
                     DFEKCQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESM
                     SWGIPCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPAS
                     IDKFYQSKYYDRLHNI"
BASE COUNT          309 a          173 c          240 g          338 t
ORIGIN      
        1 gagtttcaat gaaaatagca tttatcggcg aagctgtttc tggatttggc ggcatggaaa
       61 cggtcattcg tgatgttatt actaccttca ggcaacaaca tatctatagt gaaatgtttt
      121 tcttttgtcg taatgataaa atggataagg gatggctgga aggtattaag tactcgtgtt
      181 cattttccaa tattcgctta ggttttttgc gtcgggcgaa acatattcat gctttaagca
      241 aatggcttca agattatcag ccagatgttg ttatttgtat tgacgttatc tcgtgtttgt
      301 atgcggccaa agcacgtaaa aaatcaggaa ttgatgtgcc agtattttca tggccgcatt
      361 tttctttaga tcacaaaaaa catgctgaat atatcacctg tgcagactat catttagcca
      421 tcagctctgg cattaaacag caaatgatta atcggggtgt accagaatcg acgataaatg
      481 ttatttttaa tccagtcgaa gctaaagaca cagtcattcc tgggcctgag gaaggtgaaa
      541 cggcaacgtt catttatgtt gggcgtatga aattcgaggg ccagaagagg gttaaagacc
      601 tgcttgatgg tttatcacaa gcgcaaggtg actggaaact ccatgtactg ggtgatggtt
      661 ctgattttga aaaatgccag gcatatggta gagaattgaa gattgataat cgaattgtct
      721 ggcatggttg gcaaaaacat ccatgggagg tagtgcgtca ggatattaag aaagtcagtg
      781 cattgttatt aacctcatct tttgaaggtt ttccaatgac cttacttgaa tcgatgtcat
      841 gggggattcc gtgtatcagt gccgattgtg tctccggtcc tggtgacatt attcaacctg
      901 atgtcaatgg ccatttgtat caaccgggag atattgctgg ttttgttact cttctaaata
      961 aatatatagc aggtgaaatt catatcgaac atgaaaaaat tcctgcgtcg atcgataagt
     1021 tttatcaatc taagtattat gatagattac ataatataat
//
LOCUS       DAPQZD010000351         1052 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPQZD010000351 DAPQZD010000000
VERSION     DAPQZD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.360"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..479)
                     /locus_tag="RO454_005047"
     CDS             complement(<1..479)
                     /locus_tag="RO454_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDY0936721.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKG
                     TEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSEL
                     SYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGR"
     gene            579..986
                     /gene="fimB"
                     /locus_tag="RO454_005048"
                     /pseudo
     CDS             579..986
                     /gene="fimB"
                     /locus_tag="RO454_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          308 a          220 c          212 g          312 t
ORIGIN      
        1 aatcttccat tagcgttggc gtaaggcgaa gatgtgttaa atgagatcat tctccccatc
       61 gtccattttg tgtaccatgg tcttccatac aaaatatcat cggtacgatc attaataagg
      121 ttcacaatct cattttttac agtctgaagt tcagctacta acaactcata acttaactca
      181 ctgtaatctg agtaaaattt ttgagcaaga aggccaagct gattccattt atagccagtt
      241 tccggaaaat cgacaggcag acctttagca tcagaagcga tccactttac aacaagagca
      301 ttccatccaa gcagatacga aacgagatca cgaacactca tctccgttcc tttggcgtgt
      361 ccgtccattg atttatctga agtaatttct ggtgggattg tgttgaggta actaattaat
      421 ttactaaaat ttttatcaat agctaaaagc agttcagctt ttgtttgcgg cacactcata
      481 agaattccct ctcatgtaaa aagagacacc ataaaggtac tgtgtggtgt ttgaaaatcg
      541 ctcaattatg taccacccac cacaacaggg gaagacccat atatatccat cgattaaaaa
      601 aaggcttttc aacaacgcat ccgctattga acaaagaagt tcaggcttta aaaaactggt
      661 tgagtatccg tacttcgtac ccgcatgctg agagcgagtg ggtattttta tctcgtaagg
      721 gaaatccgct ttctcggcaa cagttttacc atattatctc gacttccggt ggtaatgccg
      781 ggttgtcact ggagattcat ccgcacatgt tacgccattc gtgtggtttt gctttggcga
      841 atatgggaat agatacgcga cttatccagg attatcttgg gcatcgcaat attcgtcata
      901 ctgtctggta taccgccagc aatgcagggc gtttttacgg catctgggat agagccagag
      961 gacgacagcg tcacgctgtt ttatagagat agttgagata ccagggatgg tgtttaaaac
     1021 tacattattc ttacgatata tcaaaaatga tt
//
LOCUS       DAPQZD010000352         1043 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPQZD010000352 DAPQZD010000000
VERSION     DAPQZD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.361"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            57..1043
                     /locus_tag="RO454_005049"
                     /pseudo
     CDS             57..1043
                     /locus_tag="RO454_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178356.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          336 a          242 c          221 g          244 t
ORIGIN      
        1 taagctcaca gagatattgc aattatctcc ggacaagcga gggaaatgct aagttgatgc
       61 aacgtatctg cggcggatat ttgaggcatt accatgccaa catctatgtg atgaaacact
      121 ttgttatacc tgagattaca aaattaagga tgaaaaaata tacttctggg agaaacatga
      181 gatcaataca gttgcggaag tttttgctgt atccacacga acgctgtatt atggcccaga
      241 ggcacctatt tcgcgtagca aagctccgct agtacatcgc acaaagcaat ggcatctcgt
      301 aattctgtaa gaaatacgtc gactgagaat agagcttccc aacctagaag aacagatttt
      361 tgtttgcttg aagccgtgga gtgaacagca ccgcctggtc tgtccgagca tttcaaccat
      421 cggcaggatg ttcacggccg cacacgataa aatgcgactg ataccgatgt gactgcctcc
      481 ccaaggaaaa acactgttca taaaaaatac aatcctccaa cccgcagatc aaaacgctat
      541 cgcccggtaa agagcagtga gctcgttggt atagatgcta ttgagctcag aatgggcgag
      601 ctgcgctgtt atgtcattac catgatcgac gaacacagca actacgctct ggcacaggcc
      661 gtgccgtcac ttaactgtga tatcatcaaa ctcttctttt gccgtgcaga ccggctattc
      721 cctatcgata tcaaccaggg catcgcagat aacgggaaag agttcccagg aaacttcgac
      781 aaagcaccgc aggaatccgc tagcaagccc tatggaccta cccctttacg cctagaacgc
      841 acctctgaac gttttaagcg aacgccgaaa gaacagttca ttgaatttaa ataaatagac
      901 tctttaaaga gctaaacata tttaatcaga aactagctga aaacctagta ctatactgca
      961 gtaaaagaca gcatgaagtg ctcgaattac tgacaccaga agaaaatatt ttacgagaga
     1021 acaaaatttg caatatgtgg cga
//
LOCUS       DAPQZD010000353         1018 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPQZD010000353 DAPQZD010000000
VERSION     DAPQZD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.362"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          331 a          202 c          175 g          310 t
ORIGIN      
        1 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
       61 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata ttcgttcaac
      121 tagccgtctc acatatgaca ttattagtca actccattta ccacaaaatg gctatttcat
      181 ggaacgcagt aatttcttag tggctattat caagctaatt cttaggtccc tagcgattat
      241 tcctggctgg ttcgtcagtt taggtcatga gattgttaca ttccttttta tcctggagat
      301 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatatg gctttggtca
      361 ctacgactta aaccctcctc aatcacagct actcgccggg cgatatggca aacaaactaa
      421 ttgaaacatc atcctgaaaa caccagcatc aacaagcctc tccagatcga catcaggagt
      481 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
      541 atggcgggta aacacttatt ttcaccaagc tcactgagtt agcttgcaaa gctttctttg
      601 tttatattct aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg
      661 aagaccaccg caagtgttcg tctggcttca catggcatct gcctcttaga aaaagatcga
      721 catattttgt gacacgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
      781 ctggtataac aggtataaag gtgcaccacg atagtcaatg aaactgggca tctcgcaatc
      841 tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact tcagtctgaa
      901 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatggattg
      961 agtaagcgat atagcttacg aaaattcaaa tcaattaaac atcagcagag attttata
//
LOCUS       DAPQZD010000354          998 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPQZD010000354 DAPQZD010000000
VERSION     DAPQZD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.363"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            439..921
                     /locus_tag="RO454_005050"
                     /pseudo
     CDS             439..921
                     /locus_tag="RO454_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(867..998)
                     /locus_tag="RO454_005051"
                     /pseudo
     CDS             complement(867..998)
                     /locus_tag="RO454_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
BASE COUNT          291 a          207 c          282 g          218 t
ORIGIN      
        1 ttattttata attctggtgg ttgtgttggc gttatcacac gctcgaaatg aagttatgca
       61 taaccgctgc ataattacct gtgtatgatt aacaataatt taacaccctt cccgataaac
      121 atgagctcgc ttagatgtca caggaaaagg gctagtatgg cagcactggt tttcgtatga
      181 aagaaagata aaacattgcc ttatatctaa ggcttcttga ttatcggtaa catccaccat
      241 ctccagccat agctggtagg tggtgacgtt ggtgcagtac tggcgggagc ttcggctccg
      301 gaactggcat acctgctgaa atcaacagaa aaagacccgg cagtaaaggc cattgctcac
      361 gccattctgg gcggtgcagt agcagcaatg cagagtaata atgttgctgc tggtgcagca
      421 ggcgcagcaa caggagagct ggcagccaga gcgatcggaa tgctgtatcc gggagtgaag
      481 ctgtctgacc tcagtgaaga gcagaagcag acgataagta cgctggcaac cgtgtcagca
      541 gggctgaccg gcggtctaac cggaaacagc accgcatcag ctgcagtggg agcacacaga
      601 gcgggaagaa tgctgttgag aataactacc tgagcgtgtc tgaaaagaca gaacttgaga
      661 tagcaaaaca gacgattaaa aacagtaaag atccggcaga gcgtgaaaag gcacagcaga
      721 aatatgatgc attgcttgaa aaagatattc agtgataagg aggttattgc tgcctgtagt
      781 aatggtaatg cgggtagttc agcgtgtgcc agcgcaagac tgaaagctat tgcgtcgaaa
      841 gaagggtacg aagatggccc ttataacgag atatcggtcg gaatgttgca gccaataagc
      901 gggtcatatt ccgtctggta gctaatcagc cccagctctg acaggaacgt cagggcacgg
      961 gtggcacggg tgatggagag tgttccggca gcagactc
//
LOCUS       DAPQZD010000355          965 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPQZD010000355 DAPQZD010000000
VERSION     DAPQZD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.364"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..>965)
                     /locus_tag="RO454_005052"
     CDS             complement(<1..>965)
                     /locus_tag="RO454_005052"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Alw26I/Eco31I/Esp3I family type II restriction
                     adenine-specific DNA-methyltransferase"
                     /protein_id="HDY0936722.1"
                     /translation="ENIKPDSRELDFFEPSMKSMYIDSLREFDDYLSRVLPYSQPKRK
                     FAGWGTNLSRVGAELSLEICNKNGLVAIVMPASFFADEQSYILREKFFNSGRIDCINY
                     YPAEAKLFGGADVSSCSFIFNKGESLNDNIQLSVYDKNLNIKSLGFFDLSSIDSQYLS
                     IPVSQGVHAVHLLRKLQEGYPTWGSLEKNGEIWAGREIDETGSSDWTQKSGGGLLFIK
                     GKMIGRYNFHNEKSLRITKKIDKVLSNSNFVRIAWRDISRPSQKRRMIATIIPPNSLA
                     GNSLGVVYYKSGSQDSLFSLLGIINSLCFEFQLRSFLATGHVSLS"
BASE COUNT          322 a          207 c          134 g          302 t
ORIGIN      
        1 ctgacaatga tacatgacca gtagctaaaa agcttcttaa ttgaaattca aaacaaaggg
       61 agtttataat accaagcaaa ctaaagagcg aatcttgcga accactttta taatagacca
      121 cccctaatga attccctgcc aaagagttag gaggtattat cgttgcaatc attcgccttt
      181 tttgactcgg gcgtgaaatg tcacgccatg caatccttac gaaattagaa ttggatagaa
      241 ccttatcaat ttttttcgtt attcttaagc tcttctcatt atgaaaatta tacctcccaa
      301 tcatttttcc tttaatgaat aataatccgc cgccactttt ttgtgtccag tcactactac
      361 cagtttcatc tatttctctt cctgcccata tctctccatt tttttccaga ctaccccatg
      421 ttgggtatcc ctcttgcagc tttcttaaca aatgaacggc atgaacacct tgagataccg
      481 gaatgctaag atattgagaa tctatactag acaaatcaaa aaaacctagt gatttaatat
      541 ttaagttttt atcatacaca ctaagttgaa tattatcatt aagggattca cctttattaa
      601 aaataaaaga acatgaagaa acatcagcac cgccgaataa tttagcctca gctggataat
      661 aatttatgca atcaatcctc cctgaattaa aaaacttttc acgtaatatg tacgattgtt
      721 catctgcgaa aaaagatgca ggcataacta tagctaccaa tccattttta ttacatattt
      781 ctaagctaag ttctgcacca acacgtgaaa gattagtccc ccacccagca aatttacgct
      841 tcggctgaga atatggcaat actcttgaca aataatcatc aaactcacga agagagtcta
      901 tatacatcga tttcatagat ggttcaaaaa aatcgagctc tctagaatct ggttttatat
      961 tctcc
//
LOCUS       DAPQZD010000356          957 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPQZD010000356 DAPQZD010000000
VERSION     DAPQZD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.365"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..829
                     /gene="yeeO"
                     /locus_tag="RO454_005053"
     CDS             <1..829
                     /gene="yeeO"
                     /locus_tag="RO454_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDY0936723.1"
                     /translation="LGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPL
                     NFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPG
                     SALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQ
                     DPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGY
                     VLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          195 a          189 c          277 g          296 t
ORIGIN      
        1 actgggattt gtcggggctg ggttgggttt aaccatttct cgttatattg gcgcagttgc
       61 aattttgtgg gtgctggcga ttggttttaa tcctgcgcta aggatttcgt taaagagcta
      121 ttttaaaccg ctgaatttta gcattatctg ggaagtcatg gggattggta ttccagcgag
      181 tgtcgaatca gtgttattta ccagtggtcg gttattaacc caaatgttcg ttgccgggat
      241 ggggaccagc gttattgccg gaaattttat cgcgttctca attgcggctc ttatcaactt
      301 acccggaagt gcgctcggtt ctgcttctac gatcattaca ggccgaaggt tgggggtagg
      361 gcagatagcg caagcagaga ttcagttgcg gcatgtgttc tggctgtcca ctcttggatt
      421 aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt tttacaccca
      481 ggatccacag gttaaacacg tcgttgtgat tctgatttgg ctaaatgctt tatttatgcc
      541 tatttggtcc gcctcatggg tgctacccgc tggatttaaa ggtgctcgtg atgcccgtta
      601 cgccatgtgg gtttcgatgt tgagcatgtg gggttgtcgg gttgtagtcg gttatgtgct
      661 gggaatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg ccgactgggc
      721 tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctat ggaaataccc
      781 tcgatccgag ccgcaaaagt gtgaaaaaaa gccagttgtg tcggaataaa cgacaaaatg
      841 cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt gtgggtgggc
      901 atcgctaata ttcgcctcgt tctcacgatt cctctgtagt tcagtcggta gaacggc
//
LOCUS       DAPQZD010000357          951 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPQZD010000357 DAPQZD010000000
VERSION     DAPQZD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.366"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(317..>951)
                     /gene="ydeO"
                     /locus_tag="RO454_005054"
     CDS             complement(317..>951)
                     /gene="ydeO"
                     /locus_tag="RO454_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDY0936724.1"
                     /translation="YERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTG
                     LLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNM
                     KLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKI
                     AEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTDNTMNALAS"
BASE COUNT          290 a          182 c          182 g          297 t
ORIGIN      
        1 gagatagcag atttagctaa aaaaagggaa ataacagtcc ataacgcgtt gacattactt
       61 tctgttctac taagtaattt ctcgccgata aacaactaat ctattgatat ttaataaatt
      121 attgtatttc actgacaaat tgccgagctg agatcataaa taatcatgca acaggttatg
      181 aaaatgcata aatatgtgat ggctgtcact tatttatttc aagaaatata tcgcctggaa
      241 acaacgcggg gcagggaatg gctgccccat ttaattctta cgcagcgtgt gtggttgact
      301 actcgttagc aaataatcaa ctagctaaag cattcatcgt gttgtccgtg ttcatacccg
      361 tgtgcctttg acaacggtac tccttagaaa ctctcttcgg actgttgccg aaatgtttgc
      421 gaaacgcata aataaaataa gatgtactgg cataaccgca ttgttcagca attttattga
      481 ctgaaccttc tacgcgtatc aaatttttcg cgtgctgcat tctggcatct aaaagaatct
      541 gtgagaatgt cgtttgctct tgctttagtt ttttcttcaa caggctttca ctgatgtaca
      601 ggcagtcaca aatatctttc agcttccatg ggtgcgccag cttcatgttg acaatatttc
      661 ttactttccc ggaaacggat agcacaccgt tagttaatag tgtaatgaaa cctttgcatg
      721 cggcaaaaat agacaggcaa gaaaaaagca aaagctctga aaaattatga tgattgcgtt
      781 cttccgagtt gagataagca atcatttcat taagcaatcc agtcggtaca tttgaagcac
      841 gtaaaaactt cgcaggaaca ggcatcgtca ctgtctggat gttagtgcat tgtagatatt
      901 ttttaatggt attttcatta aatccgattt ctttgaaatg ccgctcataa g
//
LOCUS       DAPQZD010000358          912 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPQZD010000358 DAPQZD010000000
VERSION     DAPQZD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.367"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..684
                     /locus_tag="RO454_005055"
     CDS             <1..684
                     /locus_tag="RO454_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936725.1"
                     /translation="EYLKDFDPLFPECVGKELKLGYKRKVDVWCEEDAALYAEFIATK
                     ERLKRGRLILKINQVWSRVYPAYIYAIEERINVRAKGRESFKYETVQEKQCKLSIMLD
                     GLKDLECEIAMISSEFEIEKHSMHYFYMIFDIERYINDCSYFVFSAAKVVKGVIQCIG
                     YIDKNNKASCDEFYSGSYECLDDDQETLNYLNESCAEGIDADKDLIRKNELLALSLKE
                     LYQLLVSKK"
BASE COUNT          308 a          135 c          206 g          263 t
ORIGIN      
        1 gaatacttaa aggactttga tcctttgttt cctgaatgcg tagggaaaga attaaagttg
       61 ggttataaac gaaaagttga tgtatggtgc gaagaggatg ctgcgctata tgcagaattt
      121 atagctacaa aagaaaggct gaaaagaggc cggttaatcc taaaaataaa ccaagtgtgg
      181 tcgcgtgtat atcctgctta tatttatgca attgaagaaa gaataaatgt cagggcaaag
      241 gggagagagt catttaagta tgaaactgtg caagaaaaac aatgtaaact ttccataatg
      301 ttagatggtc tgaaagacct tgaatgtgaa atagctatga tttcaagtga gtttgaaatt
      361 gaaaaacact ctatgcacta tttttatatg atttttgata ttgaacgcta tataaatgac
      421 tgcagttact ttgttttttc tgctgctaaa gttgttaaag gcgtgattca atgcattggt
      481 tacattgaca aaaataacaa agcaagttgc gatgaatttt attcaggcag ctatgaatgc
      541 ttggatgatg atcaggaaac actgaactat ttaaatgaat catgcgcaga gggtatagac
      601 gctgataaag atctcatcag gaagaatgag ttattagcct tatccctgaa ggaattgtat
      661 caacttctcg tttcaaaaaa ataacaagta atacggcgag cgatatgacg tgccatggga
      721 tatccgcacc cagcagcatg ttgtctccac gatgtccgca gttcgctcat aatagacatt
      781 cactacagtt atagtagaag gtatgcatgc tgctgagtgg ggaaagtgtg aaagaaaaga
      841 agacagctgc tttgtttgtc gtcacgttta tcttcattgg ctatgcaagt cgtaatacaa
      901 ggcgggacaa aa
//
LOCUS       DAPQZD010000359          896 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPQZD010000359 DAPQZD010000000
VERSION     DAPQZD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.368"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..289
                     /gene="iraM"
                     /locus_tag="RO454_005056"
     CDS             <1..289
                     /gene="iraM"
                     /locus_tag="RO454_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDY0936726.1"
                     /translation="SFSVIWSKIKLIIWYQSDAFLPPESIFTLTHTGIMLNNKVLPVT
                     IYNVVPFNKTFWNLIKNSQECPTNTDNVMNECFNNRCTLQICPYGLKQQSP"
     gene            complement(570..701)
                     /locus_tag="RO454_005057"
     CDS             complement(570..701)
                     /locus_tag="RO454_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDY0936727.1"
                     /translation="MIAYCFCEWHCCSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          286 a          236 c          148 g          226 t
ORIGIN      
        1 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
       61 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
      121 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
      181 aaacagccag gaatgcccta caaatacaga taacgtaatg aatgaatgct ttaataaccg
      241 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
      301 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
      361 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
      421 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
      481 tcttttaaca aactccccgc aaaccagaca acaatcaacc gcctgaattg tgaagtattt
      541 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
      601 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac tgacattatc
      661 cggctgcagc aatgccattc acaaaaacag taagcaatca ctttttaccg taaccggtga
      721 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
      781 tggctattct gttgtactcc tgaacaagag taaataattc tgaattagcc accatgaact
      841 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaa
//
LOCUS       DAPQZD010000360          884 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPQZD010000360 DAPQZD010000000
VERSION     DAPQZD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.369"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(69..314)
                     /locus_tag="RO454_005058"
     CDS             complement(69..314)
                     /locus_tag="RO454_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDY0936728.1"
                     /translation="MTITIYGRDNCSYCKRAVELAKQLKGHGYGDYEYIDITTAGLDK
                     EKLSEIVGKSVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            604..>884
                     /locus_tag="RO454_005059"
     CDS             604..>884
                     /locus_tag="RO454_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936729.1"
                     /translation="MTILENPDANVESVYSLHPTTLFHFTKNEDAFYSILAEKYFKPF
                     LAREEIRGVGGRRRFAVPMVSFCDIKLSQIRDHSGKYGEFGLGLTKSWA"
BASE COUNT          237 a          167 c          212 g          268 t
ORIGIN      
        1 tttgaatttt aagggaaaca ggagtgagtt tggtgggaat aaaaaacggc tcctgtgagc
       61 cgttaaaatc agagggtgct gacgtatgca gccagttctg tgtatccgcc aatcggttgg
      121 ccatcgatca gcacttgggg gattgtctct accgatttac caacaatttc acttagtttt
      181 tctttgtcga gaccggcagt tgtgatgtcg atgtactcat aatcaccgta gccatgtccc
      241 tttagttgct tcgccagttc gaccgcacgt ttgcagtatg agcagttatc tcgtccatag
      301 atagtgatcg tcattgcttt gcctcgttat gcgcttcgct ggggaacgtg tagcggttag
      361 tgaaagagct gaggtggtag tgctcgtgaa tgtatggatt tgacttctgc acctgacgag
      421 atgcgcgacg cgcatgttta catttatttc ccatattgag tcctttcaag tgagtatccg
      481 aaaaaactat acagttaacg tgcgtaaaaa gtaagtgctt acctatcatt tattgataga
      541 agaatagccg cctgtcgtgt gatactattt acagaactaa actggctttc cacaaggtgt
      601 aaaatgacta tccttgaaaa tcctgatgcg aatgttgaat ctgtttatag tcttcatcca
      661 accacgttgt ttcattttac aaaaaacgaa gatgctttct atagcatatt ggctgagaaa
      721 tattttaaac catttcttgc tcgcgaggaa ataagaggag tcgggggacg taggaggttt
      781 gcagtaccaa tggtatcgtt ttgtgacata aaactatcac aaatcagaga tcatagcgga
      841 aaatatggag aattcggtct tgggctaact aagtcttggg cgga
//
LOCUS       DAPQZD010000361          870 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPQZD010000361 DAPQZD010000000
VERSION     DAPQZD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.370"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..722)
                     /locus_tag="RO454_005060"
     CDS             complement(<1..722)
                     /locus_tag="RO454_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936730.1"
                     /translation="MAFDGSSDITLKASHVGAFALGKTGSTVANDKAVGWNWSSGAYN
                     ANIGGASTLIIHFYMGDGSCPAAQFRINYKNGGIFYRSARDGYGFEADWSEFYTTTRK
                     PSAGDVGALPLSGGQLNGALGIGTSSALGGNSIVLGDNDTGFKQNGDGNLDVYANYVH
                     VMRFVPGSIQSNKTINITGRVNPSDYGNFDSRYVKDVRLGSQQYYGVNNWRTWNFQCP
                     SGHVLSGINVQDTGSNSADNIAG"
BASE COUNT          249 a          216 c          164 g          241 t
ORIGIN      
        1 cccgctatat tatcggcaga gttagaccct gtatcctgaa cattaatacc agacaataca
       61 tgacctgaag ggcactggaa attccatgtt cgccagttgt tcactccata atattgctgt
      121 gaaccaagtc gaacatcttt cacataacgg gaatcaaagt taccgtaatc cgagggatta
      181 acacgccccg taatatttat ggtcttatta ctttgaatgc ttccgggaac aaagcgcata
      241 acatggacgt aattagcata aacatccaga ttaccatcgc cattttgttt aaagcccgtg
      301 tcattatcac ccaatacaat cgaattaccg ccaagagcac tggatgttcc gatacccagt
      361 gcaccattca gttgaccacc agataacggc agtgcaccaa catctcctgc tgaaggtttt
      421 ctggtggtgg tgtaaaattc ggaccagtcg gcttcaaaac cataaccatc acgggctgaa
      481 cgataaaaaa taccgccatt tttataatta atccgaaact gagctgcagg acaacttccg
      541 tctcccatat aaaaatgaat aattaacgtt gatgcaccac caatatttgc gttataggct
      601 ccgctactcc agttccatcc aactgcttta tcattcgcaa cggtgcttcc tgttttccct
      661 aaggcaaacg caccaacatg acttgctttt aatgtgatat cggaggaacc atcaaaagcc
      721 acattgctta ttttcctggc agtttttaat tttgcagctg tagaagcatt gccggatagt
      781 tcaccagaaa ggccaccgct gaatgtttgt cgattagtcc aggtattcgc tgtactgagt
      841 aacggtattt tctccccgct tgtgccgagt
//
LOCUS       DAPQZD010000362          865 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPQZD010000362 DAPQZD010000000
VERSION     DAPQZD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.371"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            19..750
                     /locus_tag="RO454_005061"
     CDS             19..750
                     /locus_tag="RO454_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDY0936731.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT          235 a          189 c          241 g          200 t
ORIGIN      
        1 gttaaaagga tattttggat gaaaacaaaa aaattgatga tggttgcact ggtcagttcc
       61 actctggccc tttcagggtg tggtgcgatg agcacagcaa tcaagaagcg taaccttgag
      121 gtgaagactc agatgagtga gaccatctgg cttgaacccg ccagcgaacg cacggtattt
      181 ctgcagatca aaaacacgtc tgataaagac atgagtgggc tgcagggcaa aattgctgat
      241 gctgtgaaag caaaagggta tcaggtggtg acttctccgg ataaagccta ctactggatt
      301 caggcgaatg tgctgaaggc cgataagatg gatctgcggg agtctcaggg atggctgaac
      361 cgtggttatg aaggcgcagc agttggtgca gcgttaggtg ccggtatcac cggatataat
      421 tccagttctg ccggtgccac actcggtgtg ggccttgctg ccggtctggt gggtatggct
      481 gcagatgcga tggtggaaga tgtgaactat accatgatca cggatgtgca gattgcagag
      541 cgtactaagg caacggtgac aacggataat gttgccgccc tgcgtcaggg cacatcaggt
      601 gcgaaaattc agaccagtac tgaaacaggt aaccagcata aataccagac ccgtgtggtc
      661 tcaaatgcga acaaggttaa cctgaaattt gaagaggcga agcctgttct cgaagaccag
      721 ctggccaaat caatcgcaaa tattctctga ttatcttccg gaggctggtc tgaccggcct
      781 cctgactgac gcctcgcttt gctcgttgtc caaccccgga aagaaataaa ataaatttta
      841 tttctccccg gcgtttccga aataa
//
LOCUS       DAPQZD010000363          846 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPQZD010000363 DAPQZD010000000
VERSION     DAPQZD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.373"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>846
                     /locus_tag="RO454_005062"
     CDS             <1..>846
                     /locus_tag="RO454_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDY0936732.1"
                     /translation="KYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTVNAAN
                     IDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAAT
                     AIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATP
                     AETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSA
                     SLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIASVN
                     GDQLTW"
BASE COUNT          274 a          163 c          177 g          232 t
ORIGIN      
        1 aaatatagtg ctgccactgg cggactcatt gctgtttctg aattagcgaa aagagtttct
       61 ggtaaaacaa accgaaaact tgtagcaaca atgttgtctc tggctgttgc cggtacagta
      121 aatgcagcaa atattgatat atcaaatgta tgggcgagag actatcttga tcttgcacaa
      181 aataaaggta ttttccagcc cggagcaaca gacgtaacaa tcactttaaa aaacggagat
      241 aaattctctt tccataatct ctcaattccg gatttttctg gtgcagcagc gagtggcgca
      301 gctaccgcaa taggaggttc ttatagtgtt actgttgcac ataacaaaaa gaaccctcag
      361 gccgcagaaa ctcaggttta cgctcagtct tcttacaagg ttgttgacag aagaaattcc
      421 aatgattttg agattcagag gttaaataaa tttgttgtgg aaacagtagg tgccaccccg
      481 gcagagacca accctacaac atattctgat gcattagaac gctacggtat agtcacttct
      541 gacggttcaa aaaaaatcat aggttttcgt gctggctctg gaggaacatc atttattaat
      601 ggtgaatcca aaatctcaac aaattcagca tatagccatg atctgttaag tgctagtcta
      661 tttgaggtca cccaatggga ctcatacggc atgatgattt ataaaaatga taaaacattt
      721 cgtaatcttg aaatattcgg agacagcggc tctggagcat acttatatga taacaaacta
      781 gaaaaatggg tattagtcgg aacaacccat ggtattgcca gcgttaatgg tgaccaactg
      841 acatgg
//
LOCUS       DAPQZD010000364          843 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPQZD010000364 DAPQZD010000000
VERSION     DAPQZD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.374"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..713)
                     /locus_tag="RO454_005063"
     CDS             complement(<1..713)
                     /locus_tag="RO454_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936733.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LSRRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRIIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEAR"
BASE COUNT          215 a          187 c          224 g          217 t
ORIGIN      
        1 cgtgcttctt ccagttcttc tggaaagtgg gcggaaataa ggcgctctgg aacgatttcc
       61 attagccaga tagcagagaa aattgcgcgt atgcgcttgc tgcgggggat gatgcgcaaa
      121 cgctccagaa tccactcaaa atagctttcc tgaatcgggt tgaactgcat gtctcccata
      181 tgcttaaagc taaccagaga gtcatccaga cgggttgttg cgcgtctgga taatttctct
      241 tcgaatatct caatcagttc tggctgccat aaatgctgtg ggcgcggcaa cttgtcccac
      301 agacgccgtg cagctgcgga aagaaccgtg gagataaagt agtcgtatga acagcaatag
      361 cgatcagcaa actggcgtgc tttccatagc gacgttttat tggcagtcga caactcctga
      421 taaggcaggc gttttaaccc ggtggtgaac ggagctgttt caaagtgttc gcgaccgtgc
      481 gtcagcatga tatttgagta ctgacgtttg tacgcctccg taaacagaca ggtggccatg
      541 agaggatgca tatcgcggta atcaaaccac ttcgtctcga agagttcagc ctcgtctttg
      601 cagcgcgaca aaccaatgtt ttcagcgacc cacttgtcca taacagcggt attccactct
      661 gtcatgaagt cgtactggtc gttgtcaatg gtatcaaaaa agatttggct catgtggctc
      721 accagatagg tagttactta cttattgtaa tgagcggatg atagcgactg gtggcagttt
      781 ttggaagtgg aaacggaagg gaatgtgtct gggaagttgg catagaaaaa gacctgcttc
      841 cgt
//
LOCUS       DAPQZD010000365          835 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPQZD010000365 DAPQZD010000000
VERSION     DAPQZD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.375"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            246..>835
                     /locus_tag="RO454_005064"
     CDS             246..>835
                     /locus_tag="RO454_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDY0936734.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFD"
BASE COUNT          243 a          162 c          202 g          228 t
ORIGIN      
        1 ggttatgaaa agagataaaa atgctgatgt caaaagaggc ttatgctcgg cacagtggag
       61 tgagctgcca aactgtcgat gactgggtag ccggtggggc ggaagtagtt atgtcccgta
      121 gcaaggttaa gatttgctct tgtgtgtggg gaaccttagt caattacttt cctggcgcac
      181 tgtgttagat tttgtaaaat tttaaaagac taaagattta atatcacttc tccatggagg
      241 ttgtggtggt agagaagatg aaacaaaatt caagttcgac gcttggcgcg ctttctgctc
      301 ctacaacgtg gccgggtgcg aacgctcggc tgcttgggtt aggtgtaggg cttccaatag
      361 cccccttgga tcgtctggca cagtttagcg ccgccgaatt tgaaaggttt acactcgaat
      421 gggcctctga atacttagct ggtcagcaat atgtcaacga agtacaacaa cgtggcgggg
      481 caggagataa aggcagagat attgttgtat ggcttgatcc atctgatgct gttcctcgaa
      541 gatggcactt atatcaatgc aaacattatg atgcgaattt aggccttcct aaggcaggca
      601 tcgaagttgc aaaagttatc tattatactt tcatcaagga ctacacagta cctacacaat
      661 accattttgt tacacataaa ggggtaacat cgccattcca agacctccta gatgatccaa
      721 ctaaactcaa agagaaaatg ctatccgagt gggcaacttt ttcaaagcaa attacatcga
      781 aacatagtgt tgacctgacg cctgaacttg aaaaatatat taaagagttt gattt
//
LOCUS       DAPQZD010000366          822 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPQZD010000366 DAPQZD010000000
VERSION     DAPQZD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.377"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..518
                     /gene="rfbC"
                     /locus_tag="RO454_005065"
     CDS             <1..518
                     /gene="rfbC"
                     /locus_tag="RO454_005065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDY0936735.1"
                     /translation="DVLIFEPKVFGDERGFFFESFNQRIFEEAVGRKVEFVQDNHSKS
                     SKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQL
                     WIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHEKNIIWNDLLLNIKWPSTALITLSDKD
                     ANGERFELSEF"
     gene            520..>822
                     /locus_tag="RO454_005066"
     CDS             520..>822
                     /locus_tag="RO454_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDY0936736.1"
                     /translation="MSLLKHSIWNVAGYFIPTLIAIPAFGLIARKIGVELFGLYTLAM
                     IFIGYASIFDAGLTRAVVREIALLKNRVDDCNTIIVTSIIAVIFLGFIGGGGVFLLK"
BASE COUNT          230 a          106 c          203 g          283 t
ORIGIN      
        1 ctgatgtgct gatttttgaa ccaaaagttt ttggggatga acgtggcttc ttttttgaga
       61 gttttaatca gaggattttt gaagaagcag taggtcgtaa ggttgagttt gttcaggata
      121 accattctaa gtccagtaaa ggtgttttac gtggtcttca ttatcagtta gaaccttatg
      181 ctcaaggaaa actggtgcgc tgtgttgttg gcgaggtttt tgatgttgcg gttgatattc
      241 gtaaatcgtc acctacattt gggaaatggg ttggggtgaa tttgtctgct gagaataagc
      301 gtcagttgtg gattcctgag ggatttgcac atggtttttt ggtgctgagt gatttagcag
      361 aagttttata taaaacgaat caatattatg ctccatcaca tgaaaaaaat attatatgga
      421 atgacctctt gcttaatatt aaatggccga gcacagcact gatcactctg tctgataagg
      481 atgcaaatgg ggaaagattt gaactaagtg agttttgaaa tgtctctctt aaaacatagt
      541 atatggaatg ttgcgggcta ctttatacca acattaattg caattcccgc ctttggatta
      601 attgcgagga aaattggtgt agaactattt ggtttgtata cgttagcaat gatttttata
      661 gggtatgcaa gtatatttga tgctgggtta acaagagctg ttgtgcgtga aatagcatta
      721 ctaaaaaaca gagtggacga ttgtaatacg ataatagtaa cttctattat cgctgtgata
      781 tttttagggt ttatcggagg cgggggagtg tttctgctta aa
//
LOCUS       DAPQZD010000367          808 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPQZD010000367 DAPQZD010000000
VERSION     DAPQZD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.378"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            19..>808
                     /locus_tag="RO454_005067"
     rRNA            19..>808
                     /locus_tag="RO454_005067"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          231 a          165 c          251 g          161 t
ORIGIN      
        1 aacatcttcg ggttgtgagg ttaagcgact aagcgtacac ggtggatgcc ctggcagtca
       61 gaggcgatga aggacgtgct aatctgcgat aagcgtcggt gaggtgatat gaaccgttat
      121 aaccggcgat ttccgaatgg ggaaacccag tgtgtttcga cacactatca ttaactgaat
      181 ccataggtta atgaggcgaa ccgggggaac tgaaacatct aagtaccccg aggaaaagaa
      241 atcaaccgag attcccccag tagcggcgag cgaacgggga ggagcccaga gcctgaatca
      301 gtgtgtgtgt tagtggaagc gtctggaaag gcgcgcgata cagggtgaca gccccgtaca
      361 caaaaatgca catattgtga gctcgatgag tagggcggga cacgtggtat cctgtctgaa
      421 tatgggggga ccatcctcca aggctaaata ctcctgactg accgatagtg aaccagtacc
      481 gtgagggaaa ggcgaaaaga accccggcga ggggagtgaa aaagaacctg aaaccgtgta
      541 cgtacaagca gtgggagcct cttaatgggg tgactgcgta ccttttgtat aatgggtcag
      601 cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta
      661 actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt
      721 gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg
      781 acttgtggct gggggtgaaa ggccaatc
//
LOCUS       DAPQZD010000368          808 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPQZD010000368 DAPQZD010000000
VERSION     DAPQZD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.379"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            118..348
                     /locus_tag="RO454_005068"
     CDS             118..348
                     /locus_tag="RO454_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936737.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNIQLHGRENNQSQ"
BASE COUNT          227 a          166 c          199 g          216 t
ORIGIN      
        1 cggaagcagg ctgttttgta tcagagactc cagacctgaa catcactaca gaatccacta
       61 gaaccccttc ccagacgctt taaaatcgat tttatgaacc actttaagga aaccaacatg
      121 tcatacccga ctaatgtcgt tgcgctcgta gagagcgatt ttctggccca ggctcgtgaa
      181 atgatgaaag atcgtgagca ggctttcaac ttgtacgaat gggcaattaa gtgcttgcat
      241 cttggcgagc atcgcgaact tgttgaacag cttttaggtg agttgatcaa tgaggtgttt
      301 gccttgaata ttcaactaca tggtagagaa aataatcaat cacaataata gataagtaca
      361 aactattcat aaagtgaatt gtaagtgcta agatctgata gtttccagtc gtagactgga
      421 gactcgacct gatgggtggg ggtaagcgtc actggcgtca ggtttaaaaa agctcactac
      481 cagcgtagaa ccggtgccgt ataggtgtcg gggaaggggg aaccaaagtg agcagagaca
      541 agggtcactt tatgattgtc gagtctgggg tgtttcgaga ggttgaatcc agtactcccc
      601 ttcataaagt gtgggaagat ctcggttctg gggtgctgtc atccataact tcccaagtct
      661 aagctggcag tagacttaga ccatagcttt tcaggttatg aaacgaccag gttggtgagg
      721 aattttatac tcacctccct gggagagtat tacctgaaaa gacaacctct cacttcgttc
      781 gaggtgaact tcactcactt cgttcgtt
//
LOCUS       DAPQZD010000369          775 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPQZD010000369 DAPQZD010000000
VERSION     DAPQZD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.380"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            10..>775
                     /locus_tag="RO454_005069"
     CDS             10..>775
                     /locus_tag="RO454_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HDY0936738.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGE"
BASE COUNT          288 a          126 c          132 g          229 t
ORIGIN      
        1 gttctaatta tgattaataa aattgatttc aaagctaaga atctaacatc aaatgcaggt
       61 ctttttctgc tccttgagaa tgcaaaaagc aatgggattt ttgattttat tgaaaatgac
      121 ctcgtatttg ataatgactc aacaaataaa atcaagatga atcatataaa gaccatgctc
      181 tgcggtcact tcattggcat tgataagtta gaacgtctaa agctacttca aaatgatccc
      241 ctcgtcaacg agtttgatat ttccgtaaaa gaacctgaaa cagtgtcacg gtttctagga
      301 aacttcaact tcaagacaac ccaaatgttt agagacatta attttaaagt ctttaaaaaa
      361 ctgctcacta aaagtaaatt gacatccatt acgattgata ttgatagtag tgtaattaac
      421 gtagaaggtc atcaagaagg tgcgtcaaaa ggatataatc ctaagaaact gggaaaccga
      481 tgctacaata tccaatttgc attttgcgac gaattaaaag catatgttac cggatttgta
      541 agaagtggca atacttacac tgcaaacggt gctgcggaaa tgatcaaaga aattgttgct
      601 aacatcaaat cagacgattt agaaatttta tttcgaatgg atagtggcta ctttgatgaa
      661 aaaattatcg aaacgataga atctcttgga tgcaaatatt taattaaagc caaaagttat
      721 tctacactca cctcacaagc aacgaattca tcaattgtat tcgttaaagg agaag
//
LOCUS       DAPQZD010000370          767 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPQZD010000370 DAPQZD010000000
VERSION     DAPQZD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.381"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            16..441
                     /locus_tag="RO454_005070"
     CDS             16..441
                     /locus_tag="RO454_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936739.1"
                     /translation="MTAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            497..>767
                     /locus_tag="RO454_005071"
     CDS             497..>767
                     /locus_tag="RO454_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936740.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKADGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTF"
BASE COUNT          242 a          162 c          179 g          184 t
ORIGIN      
        1 aaaggataag aaaacatgac agctattaag aaactctacg atgccgcaaa cgtggctctg
       61 gatgttattg atgatgaagt agcaaaaggc tttcctgaac ctgattgggc gcatcagcta
      121 cgaaacgcta tcgcagaaat gaccccacca gatccaaccc ccgacgagac agactggcag
      181 cgattcatcc gtatgtacgc tcaggaaata ggcccaacgc caacggcaga gcaggcaatg
      241 ctgctgaaat acttcaaaga ggcgggagag gatttaccaa ttgatgactc agcatattgg
      301 ttccactgcg catggcgtaa gtatgacgtg atattcacac aaggcatggg aagcaaagat
      361 atggttgtgt ggcatctact ccatatagac acagccgttg acagagttat tgaacagttt
      421 ttccctaaac aagaagattg atcgcctatt cataactaac aaaataagta aacactaacc
      481 acaaaaggaa aaacacatga gagttttagt tcgaatcgtt accagcactg tctatgacgt
      541 gtttccgctt tttatggtca aagccgatgg ccttaacgac gaagaaactg acgcgctgat
      601 ccagcgtatt ctcgttgaat atacaggtca tgacgctgat tcagtgatgg ttgatgatga
      661 tggtgtttgt tggcataacg gcaactgttg gtacgtagaa gagactcaac aaatcagtga
      721 tgaagatgcc gcacatcttg agcgtatttt aagcatcagc acttttg
//
LOCUS       DAPQZD010000371          759 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPQZD010000371 DAPQZD010000000
VERSION     DAPQZD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.382"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            90..644
                     /locus_tag="RO454_005072"
     CDS             90..644
                     /locus_tag="RO454_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /protein_id="HDY0936741.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGRWEEETDPGVRGIDQL
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTV
                     TTPYGPAVYMVQTRQAFERTRSDA"
BASE COUNT          205 a          197 c          207 g          150 t
ORIGIN      
        1 taagcgtgca taataagccc tacacaaatt gggagttaga catcatgagc aacgcaaaaa
       61 caaagttagg catcacaaag tacagcatcg tgaccaacag caacgattcc gtcacactgc
      121 gcctcatgac tgagcatgac cttgcgatgc tctatgagtg gctaaatcga tctcatatcg
      181 tcgagtggtg gggcggagaa gaagcacgcc cgacacttgc tgacgtacag gaacagtact
      241 tgccaagcgt tttagcgcaa gagtccgtca ctccatacat tgcaatgctg aatggagagc
      301 cgattgggta tgcccagtcg tacgttgctc ttggaagcgg ggacggacgg tgggaagaag
      361 aaaccgatcc aggagtacgc ggaatagacc agttactggc gaatgcatca caactgggca
      421 aaggcttggg aaccaagctg gttcgagctc tggttgagtt gctgttcaat gatcccgagg
      481 tcaccaagat ccaaacggac ccgtcgccga gcaacttgcg agcgatccga tgctacgaga
      541 aagcggggtt tgagaggcaa ggtaccgtaa ccaccccata tggtccagcc gtgtacatgg
      601 ttcaaacacg ccaggcattc gagcgaacac gcagtgatgc ctaacccttc catcgagggg
      661 gacgtccaag ggctggcgcc cttggccgcc cctcatgtca aacgttgggc gaacccggag
      721 cctcattaat tgttagccgt taaaattaag ccctttacc
//
LOCUS       DAPQZD010000372          758 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPQZD010000372 DAPQZD010000000
VERSION     DAPQZD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.383"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..570)
                     /locus_tag="RO454_005073"
     CDS             complement(<1..570)
                     /locus_tag="RO454_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDY0936742.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSAENSGNQVIFTLYVPPDTGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVVYDNYVTPEEADKKYLQISKNL
                     SEIPANGETAQQAARKNIGIDGDVAYRDKG"
BASE COUNT          179 a          193 c          156 g          230 t
ORIGIN      
        1 acccttatct ctatatgcaa cgtccccatc tataccaata tttttccgcg cagcctgctg
       61 agcagtttca ccatttgcgg gaatttcaga taagttttta cttatctgca aatatttttt
      121 gtcagcttcc tcgggagtta cgtagttgtc atatactaca acattaaccg tagatgtgct
      181 ggacaccgcg agtgtgtatt tatacgtgat tgaaatcagc gcaccgttgc tgtccgtagg
      241 ttttaaaatg tccggcgagc gcgccacaga gtacagctcg cctttatctg ttaatatccc
      301 cacctcacgg atagtatagc ccccagtgtc tggcggtacg taaagtgtaa agataacctg
      361 gttaccgcta ttttctgcgg atttaatatc cccacgatac gtctcgtgaa ttaatgcagt
      421 tcgtgccggg tccggtgtta cgtcgtcgtt tccgtttgcg tcgccaataa cgaatttatt
      481 cagcaccacg ggtacacccg atgccagcgc agcggcttca agttgtgcgc cacggtctgt
      541 cagtactgcg taaattttat tttcagccat ttggccctat ctccacattt aaactaacaa
      601 ttggtgagcc cgatatatat agcccgccgt taatttttac accgccgtcg ccgtctgctg
      661 ggacgtttac gcctattttc gcgtaaggtg taccggctat aaaaaactca ccatcgacac
      721 ccgtttcgaa atttatttct tttaataagg aacgacaa
//
LOCUS       DAPQZD010000373          752 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPQZD010000373 DAPQZD010000000
VERSION     DAPQZD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.384"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(129..>752)
                     /locus_tag="RO454_005074"
     CDS             complement(129..>752)
                     /locus_tag="RO454_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDY0936743.1"
                     /translation="SQGYTLFVLSYRLPSPVEGENINKTFIDAISAMLYIRKNADKWH
                     LQRHRIGVLGFSAGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDD
                     VTHPGSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRG
                     LIEKGVSAEMHLYEKGGHGFSIQWATDLSVGGWIKLFMDWMYSHGFK"
BASE COUNT          257 a          159 c          123 g          213 t
ORIGIN      
        1 attgaaatac ttacggccat aaaagagcat gattgtgata aagctgaaca gctaatgtat
       61 aatcaccttt ataaacgaat tgaagcatca tagttactcg acgcttcacc aggtagtcac
      121 cataaatatt atttaaatcc atgagaatac atccagtcca taaacagttt tatccatcct
      181 ccaacagaaa gatctgtggc ccactgaata ctaaaaccat gccctccttt ctcatagaga
      241 tgcatttcag cagaaacgcc tttctctatt aggccacggt acatgagcag actgttttca
      301 attgacacaa gatcatcatc acaacaatgg agtaaaaaca caggaggaaa acatgatgtg
      361 acattaagat ccgcactata ttgtattata ttttctgagg tactattgtc ccccagtaat
      421 tgcttcctgg agcctggatg tgtcacatca tctttcattg atattacagg ataaattaac
      481 ccgacaaaat cgggaatagc gaaaatctct tgttctttac tgtcagaaga acgtttgaaa
      541 tcacaaatct gattcacaaa cgttgaggcg atatgccccc cagcagaaaa tcctaataca
      601 ccaatacgat gacgctgtag atgccattta tcagcattct tacgaatata aagcattgca
      661 gatattgcat caataaaagt tttgtttata ttttctccct caactggcga tggtaatctg
      721 taactaagaa caaataatgt atagccctga ga
//
LOCUS       DAPQZD010000374          743 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPQZD010000374 DAPQZD010000000
VERSION     DAPQZD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.385"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(60..623)
                     /gene="ipbA"
                     /locus_tag="RO454_005075"
                     /gene_synonym="fimX"
     CDS             complement(60..623)
                     /gene="ipbA"
                     /locus_tag="RO454_005075"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDY0936744.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          231 a          150 c          149 g          213 t
ORIGIN      
        1 tttatggaag aattgaggaa aacgtaggaa tctgacattt tgaatcagaa ggtactgttt
       61 taacgaggct tttttttcca caccccttta aaacgagcag cattactggc tgtgtatctg
      121 acagtatgct gaatatttcg atgacccaga taatcctgaa gtagtcgggt atcgacacca
      181 ttgtcagcca gagcaaaacc acaggcatga cgtaacatat gaggatgtgc ttttacagct
      241 aatcctgcac gttttccagc ctcacgaatg atagaaaaaa aatgttgtct gctgataggg
      301 cggcgtttcc gggagagaaa aagccagtct ccttcaactc cgttttcaat taacttcctc
      361 tgcttaagcc acaacttgat caaattatat tcatcaggaa gaagaggatg tgttgtcgag
      421 aaaccatttt taatacgccg aatattaagt tgttttccag atgcatcaat atcggataaa
      481 cgtaaatcaa gaagttcact ggccctaaaa ccgtgaatga aagccatcat aattagacag
      541 cgatttcttt caggaaagga catgctctgg gctgcatcca tcagcctgta gacttcatcc
      601 tgtgtgagat attttctcgt catacgccac cgtgtacagt tgtaaaaaaa agagaggcac
      661 acccagataa gtatgcctgc aatcagaacg ttatcgatgt aatctcatta tctcattaat
      721 aagatttttt gaatagtagt tct
//
LOCUS       DAPQZD010000375          738 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPQZD010000375 DAPQZD010000000
VERSION     DAPQZD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.386"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(1..>738)
                     /locus_tag="RO454_005076"
     CDS             complement(1..>738)
                     /locus_tag="RO454_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDY0936745.1"
                     /translation="REGRALYDNLHPSRIIIGECSERAERLAVLFQEGAIKQNIPVLF
                     TDSTEAEAIKLFSNTYLAMRVAFFNELDSYAESFGLNTRQIIDGVCLDPRIGNYYNNP
                     SFGYGGYCLPKDTKQLLANYQSVPNKLISAIVDANRTRKDFITNVILKHRPQVVGVYR
                     LIMKSGSDNFRDSSILGIIKRIKKKGVKVIIYEPLISGDTFFNSPLERELAIFKGKAD
                     IIITNRMSEELNDVVDKVYSRDLFKCD"
BASE COUNT          245 a          158 c          115 g          220 t
ORIGIN      
        1 ttagtcacat ttaaacaaat cgcgactata gactttgtcg accacatcgt tcaactcctc
       61 tgacattcgg ttagtgataa taatatcagc tttcccttta aagatcgcca gctcccgttc
      121 caaaggtgag ttaaagaatg tatctccaga aataagcggc tcataaataa ttactttcac
      181 gccttttttc ttgatacgct ttataatacc aagaatagaa gaatctctaa aattatctga
      241 accacttttc ataatcaaac gataaacccc cacaacttgt ggtctatgtt tcaaaataac
      301 attagtgata aagtccttac gtgtacggtt agcatcaaca attgcagata taagtttatt
      361 cggaacagac tgatagttgg ctaataattg cttggtatct tttggcaaac agtagccacc
      421 ataaccaaaa gaaggattat tgtagtaatt accaatgcgc ggatccaaac aaacaccgtc
      481 aataatctga cgcgtattca gaccaaaact ttctgcgtaa ctatccaatt cattaaaaaa
      541 tgcaactcgc atagccaaat aagtatttga aaataactta atcgcttccg cttccgtaga
      601 atctgtaaat aaaacgggta tattttgttt aatcgctcct tcctgaaata acactgccaa
      661 acgttctgcc cgttcagaac attcaccgat aataatgcga gatggatgga gattatcgta
      721 tagggctctt ccttctcg
//
LOCUS       DAPQZD010000376          737 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPQZD010000376 DAPQZD010000000
VERSION     DAPQZD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.387"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>737
                     /locus_tag="RO454_005077"
     CDS             <1..>737
                     /locus_tag="RO454_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDY0936746.1"
                     /translation="HSERNILTASLIIKNEGSYAINWQDFDLKIKSAKMVLLCNPHNP
                     TGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISK
                     TFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIGYNECEGWLKSL
                     KAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEGKVG
                     IMAGHVYGTEGEGYLRLNLACSREKLY"
BASE COUNT          237 a          124 c          155 g          221 t
ORIGIN      
        1 catagcgagc gaaatattct gacagcctct ttgattataa aaaatgaggg gagctacgct
       61 atcaactggc aagattttga tcttaagatt aaaagcgcaa agatggtgtt attatgtaat
      121 cctcataacc cgacaggaac agtctggtcg gaagaagaac tgcataaaat cgctgcaagc
      181 tgtacaaggc ataatgtctg gttatgttca gatgaaatcc atagtgattt tgtttttaat
      241 cgtagcttta catccgcgct taaaataaaa aaagaaaaag tagtagtatt taactcgata
      301 tcgaaaacat ttaatgttcc tgcactaaca ggatcgtata tgatttcaac tgacgataat
      361 tttaatcata agttcagaac gatatcgagg tatcgcgatt ttgtaaactc cccatcagta
      421 ctgaatataa ttgcaactat tattggctat aacgaatgtg agggatggtt aaagtcatta
      481 aaggcacata ttgcctctaa cattcaattc acccagcagt atctgaatga aaatattcca
      541 gaattgatcg taaggccagc tgatggttgc tatttttcct ggatagattg ttctgctatt
      601 ggttatcctt ttgatgaatt ctacagtcga ttaattcatg aaggcaaagt gggcattatg
      661 gcggggcatg tctatggaac ggagggggag ggctaccttc gtctgaacct ggcctgcagt
      721 cgagaaaaat tatatat
//
LOCUS       DAPQZD010000377          728 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPQZD010000377 DAPQZD010000000
VERSION     DAPQZD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.388"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>728
                     /locus_tag="RO454_005078"
     CDS             <1..>728
                     /locus_tag="RO454_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDY0936747.1"
                     /translation="TDAFPTYIPISSTKKFCDEFDIMIGRYGPPIFQICRGLKGAYNV
                     ALLKVIPKEGVSRDFLYYFLKQDSVFQYVDKLSARTGGQTGVDLVSLKEYPVRIPEEI
                     ECQEKLVTILSVIDKKIALNNRINTELEAMAKTLYDYWFVQFDFPDANGKPYKTSGGK
                     MEYNATLKREIPAGWNDSILGKFIELDRGVTYSKEDVRTQDDKDTIGILRATNVTGNN
                     VDIDDLVFIPSSRVNVNQMLNKFD"
BASE COUNT          233 a          121 c          158 g          216 t
ORIGIN      
        1 acggatgcat ttcccacgta tataccaata tcgtcgacga agaaattttg tgatgaattc
       61 gatattatga ttggtaggta tggtccccca atttttcaaa tatgtcgcgg attgaaagga
      121 gcatataacg ttgccttgct gaaggttata cctaaagaag gtgtaagtcg tgatttttta
      181 tactattttt tgaagcaaga ctctgttttc caatatgttg ataaattatc tgccagaacc
      241 ggaggacaaa ctggagttga tctagtttct ttaaaagaat atccagtcag gatacctgaa
      301 gaaattgaat gtcaagaaaa attggtgact attcttagtg ttattgataa aaaaatcgcc
      361 ctcaacaacc gcatcaacac ggaactggaa gcgatggcga aaacactgta tgactactgg
      421 tttgtacagt ttgatttccc tgatgccaac ggaaaaccgt ataaaacctc cggcgggaag
      481 atggagtata acgccacgct gaaacgggaa attccggcag gttggaatga ctccattctt
      541 ggtaagttta ttgagttaga tcgtggcgtt acttatagca aagaggatgt tcgaactcag
      601 gatgataaag atacgatagg cattcttcgc gctacaaatg tgacgggaaa taatgttgat
      661 attgatgatt tagtcttcat tccttctagt agagtaaacg ttaatcaaat gctaaataaa
      721 tttgacat
//
LOCUS       DAPQZD010000378          711 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPQZD010000378 DAPQZD010000000
VERSION     DAPQZD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.389"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>711
                     /locus_tag="RO454_005079"
     CDS             <1..>711
                     /locus_tag="RO454_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HDY0936748.1"
                     /translation="AVTYGCSYSRYADDITFSTNKKNIPDALVSNEKENEPGKILVEE
                     IHRAGFTLNHNKNRVSRCTSRQQVTGLTVNKKINVSREYIKNTRAMAHSLYFEGSYTL
                     IEKDGKHRKGTLSELEGRFAFIDMLDKYNNVEAKKNARPERYVVKGFGLDFKQRLNSR
                     EKAYSKFLYYKNFYGNEQITILTEGKTDPVYLKCAIDSLFLDYPQLVREEKNTKNRVL
                     KVNLFKTNDKKKYFLDLSG"
BASE COUNT          276 a           97 c          142 g          196 t
ORIGIN      
        1 gcagtcacat atggttgtag ttacagccgc tatgcagatg acattacgtt ttcaacaaac
       61 aaaaaaaaca tccctgatgc attagtttct aatgagaaag aaaacgaacc aggtaagata
      121 ttggtagaag aaattcatcg tgcaggcttc actttaaacc ataataaaaa cagagtgtct
      181 aggtgtacat caagacagca agttacaggt ttaactgtaa ataaaaaaat aaatgtaagc
      241 agagagtata taaagaatac aagagcgatg gcgcattctt tatactttga aggttcgtat
      301 acacttattg agaaagatgg aaaacataga aagggcaccc ttagtgaatt agaagggcga
      361 tttgcattta tcgatatgct tgataaatat aataatgtgg aagcaaagaa aaatgcgcgt
      421 cctgagagat atgtggttaa aggatttggg ttggatttta agcagagact taactccaga
      481 gagaaagcat acagcaaatt cctatactat aaaaatttct atggaaatga gcaaataaca
      541 atcttaacag aagggaaaac tgacccggtt tatcttaagt gtgcaattga ttctttgttt
      601 ttggattacc ctcagttagt tagagaggaa aaaaacacaa agaatagagt gttaaaagtt
      661 aatttattta aaaccaatga caagaaaaaa tattttctcg atttgtctgg t
//
LOCUS       DAPQZD010000379          711 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPQZD010000379 DAPQZD010000000
VERSION     DAPQZD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.390"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            113..697
                     /locus_tag="RO454_005080"
     CDS             113..697
                     /locus_tag="RO454_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDY0936749.1"
                     /translation="MSKKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKS
                     ATYDFKASYVRADASLAATAGYVKTNTAYTVTYQ"
BASE COUNT          206 a          147 c          146 g          212 t
ORIGIN      
        1 gtaaatcttc acattcatca ttaacaaact aatctctttt cctctatcgg gaatccctgc
       61 ctgaaagggc ccaattgtag ttaaactcat ggaatatgat tcaaggattt ttatgtctaa
      121 aaaattaggt tttgcgctct ctggtcttat gctggcaatg gttgcaggta gtgcttctgc
      181 agatatggac ggtggtcagt taaatatcag tggtctggtt gttgataata cctgtgaaac
      241 ccgcgtagac ggcggtaata aagatggcct gatcctgctg caaactgcaa ccgttggaga
      301 aatctctgag ggtgtgctgg acactaccgt tggtgctaag gctaaaccat tcagcattac
      361 tgttgactgt agtaaagcta atccggcacc aggcagcaca gcaaaaatga cttttggttc
      421 agtcttcttt ggtaacagta aaggaaccct gaataatgac atgtcaatta atactccatc
      481 tgacggtgtt aatattgctc tgcacaatat tgaaggttct actattaagc aggttaaaat
      541 caacaatcct ggcgatatct attccaaagc actggatgca acttccaaat ccgcaactta
      601 tgattttaaa gcgtcttatg ttcgtgcaga tgcaagtctg gcagccacag caggttatgt
      661 aaaaactaac actgcatata ccgttactta tcagtaatca cttccattca t
//
LOCUS       DAPQZD010000380          709 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPQZD010000380 DAPQZD010000000
VERSION     DAPQZD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.391"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..530)
                     /gene="fimE"
                     /locus_tag="RO454_005081"
     CDS             complement(<1..530)
                     /gene="fimE"
                     /locus_tag="RO454_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDY0936750.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAA"
BASE COUNT          220 a          175 c          140 g          174 t
ORIGIN      
        1 gcagcattac tggcggtata acgcacagta tggcgaatat ttcgatgccc gagataatcc
       61 tgaattaaac gagtatctgc accacgctcc gccagttcat aaccgcaagc atgccttaac
      121 atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg aataatgcga
      181 taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc gtcagtccgg
      241 tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc acgctcatca
      301 aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt tattctacct
      361 tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg catcccatgc
      421 cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta acaaaccgcc
      481 tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac aatagtagta
      541 ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg tcaaaattag
      601 cccaatagat aaaaagataa ccacacacgg agtgatgtgg ctatcagtca attacattaa
      661 atatcataca aaaataaaat atcactgatg tgacaacatc tattttcat
//
LOCUS       DAPQZD010000381          686 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPQZD010000381 DAPQZD010000000
VERSION     DAPQZD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.392"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            2..559
                     /locus_tag="RO454_005082"
     CDS             2..559
                     /locus_tag="RO454_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDY0936751.1"
                     /translation="MRNEHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGVGKSGII
                     ARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKK
                     MGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALS
                     ELKKFTRADFGLYHPGGALGIKANS"
BASE COUNT          171 a          141 c          162 g          212 t
ORIGIN      
        1 tatgcgtaac gaacataaag cagttgaaga agttattgat tcccctcttt cggaggtcgc
       61 aaatttaatt aaagtattac aatcttgtca gggaaaagtt gtttttattg gtgttggtaa
      121 gtccggtatt attgccagga aactcgccgc aacatttgcc agcaccggaa ctccctcgtt
      181 ttttgttcac ggtacggaag ctgtacacgg cgaccttgga atggtggcga aagacgatgt
      241 tgttattctt atttcaaaca gtggtgagac ggcggaaatc ctggcgacac tgcctagtct
      301 gaaaaaaatg ggtaattatt tgatctcttt tacccgcagt caccactcat cgcttgcgat
      361 aagttgtgat ttatctgttg aaatccccgt caaaagtgag gcggataatt taggtttagc
      421 accgtcatgc tcgtcgactg ttgttctggt tgttggtgat gcggtggcgc ttgcactctc
      481 tgaattgaaa aaatttactc gtgctgattt cggcttatat catccaggag gtgcactcgg
      541 cattaaagca aattcataat ttcaaaatat aaatacagta accggtcacg ccatcttttg
      601 gggtggcgtt ctttgaagtg agtgtccgct atgaaaaatg ttctggtcac cgtccccagc
      661 ttttcagcac gctgtgtttc cgccag
//
LOCUS       DAPQZD010000382          686 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPQZD010000382 DAPQZD010000000
VERSION     DAPQZD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.393"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            75..686
                     /locus_tag="RO454_005083"
                     /pseudo
     CDS             75..686
                     /locus_tag="RO454_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          161 a          149 c          167 g          209 t
ORIGIN      
        1 tttatcttcg gcgtggatat ttgtgatgct ctctaccatg ctatctgtag accccagcgc
       61 gagcgaatga acgtctcttt agcttctaca gacttatcga taaaggcttt gggattttga
      121 tatttattct gttaatcgcc tgcgccgctg ccttccttct tgataggtat ttcaataaaa
      181 gcgcaacgcc tgaagagatc ctgcgacggg ctataaataa tggggagatc gtcccttttt
      241 accaacctgt ggtaaatggt cgggaaggga cattgcgggg agttgaggtg ttagcccgct
      301 ggaaacaacc tcacggtgga tatatatcac ctgcggcatt tattccactt gctgaaaaat
      361 gcggattaat cgttccgctt acgcaaagcc tgatgaatca ggttgccacc cagatgaacg
      421 ctatcgcgag taaactgccg gaaggttttc atattgggat taattttagc gcctcgcata
      481 ttgtttcgcc gacgtttgtc gacgagtgct taaattaccg tgacagtttt acccgccgcg
      541 atttaaacct tgttctggaa gtcaccgagc gtgaaccatt aaatgttgat gaaagtctgg
      601 ttcagcggtt gaacattctg catgaaaatg gttttgtcat cgcgctggat gatttcggta
      661 ctggttactc agggctttct tatctg
//
LOCUS       DAPQZD010000383          685 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPQZD010000383 DAPQZD010000000
VERSION     DAPQZD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.394"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(134..658)
                     /locus_tag="RO454_005084"
     CDS             complement(134..658)
                     /locus_tag="RO454_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDY0936752.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVT
                     LPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYV
                     YPALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISNNYNKNHT
                     GAWSAQYTLTATYN"
BASE COUNT          208 a          122 c          133 g          222 t
ORIGIN      
        1 accagtctca gctagcgaaa tatgagaaaa aaatatgaaa gataatattg taatattgta
       61 ttttttcatt ttatattgtc acattaaaga atgctgaata tacaggcatg agtaatcatg
      121 cctgttaaat atattagtta taagttgcag tcaaagtgta ttgcgcactc catgcaccag
      181 tatgattttt attgtagtta tttgatattt taatcatttg caactcgatt ggatatgtac
      241 cattagcaag ttcgattggt gcattataag aagatacacc tttatcaccg actttcatcc
      301 aaagcgcaaa atccttttgt tgattaccgg tagcctcgtt tagagcagga taaacatagc
      361 cattagactg gaaattagta gttgctaccg ttagtgatgc aggagtggta caatcggtaa
      421 cactaatccc ctgatctgcg gttacacttt ttattttctg gtacgctttt gcagcattta
      481 cctcttgatt cgttactgtg ccaaaagatg ctgttgcatt aggcagggta acactgcaag
      541 tcgcggcatt aaaagaacca gtagcggaga cagaaccact gcttgtctgg gccattgcgg
      601 taccagaaat aagcagagcc gaaatagctg cgatggtgat tttattctta gaaaacattt
      661 tcaacctcaa tcttgatttt cttaa
//
LOCUS       DAPQZD010000384          685 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPQZD010000384 DAPQZD010000000
VERSION     DAPQZD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.395"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            24..>685
                     /locus_tag="RO454_005085"
     CDS             24..>685
                     /locus_tag="RO454_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936753.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GNFDESAKTFIDFVAKRWSEQWKDMEKRASEFDRFMDAMDTAKEALAAGTPLDLESLF
                     NGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQRKEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HL"
BASE COUNT          191 a          144 c          179 g          171 t
ORIGIN      
        1 actaaacgat aagagaaaaa cacatgaaca acctccagtt agagcatttt aacgtcacag
       61 gccattctga ttttcctttc aagtttacat tgaaaggtta tgcagaggat gcggtaggcc
      121 agatcattat tgataaaggc atcgttaagt ttgaggggaa ttttgatgaa tccgcgaaaa
      181 cattcataga cttcgttgcc aaacgttgga gcgagcaatg gaaagacatg gaaaagcgcg
      241 ctagtgagtt tgatcggttc atggatgcaa tggatacagc aaaagaggct cttgctgccg
      301 ggactccgtt agatcttgag tcacttttca acggcgaagt ggcctctgcg atgttcgcca
      361 ccatgttcgc gggtgagttc gtccgcagcg gtgccaaaaa ctaccttgag ctggattaca
      421 acgtccctgc aattggcgat ttcgtcgtta ccatccaacg caaagaaggt aagacgccag
      481 gtgaacgcgt cgcagagctt gaggccgttg tggatcagcg taacggagag tgtgaccgtt
      541 tgatcaacga gcttcatgca cttcgggaag aaagaatatg tgcgggtagc aatacacgta
      601 atgcagcgga tatctacttc cagttagttg aggaatgcca gattccacca ggtggctctc
      661 ttgtcgatta cgtcagacat ttaat
//
LOCUS       DAPQZD010000385          678 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPQZD010000385 DAPQZD010000000
VERSION     DAPQZD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.396"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            222..>678
                     /locus_tag="RO454_005086"
     CDS             222..>678
                     /locus_tag="RO454_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDY0936754.1"
                     /translation="MDVPVLNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT          190 a          180 c          153 g          155 t
ORIGIN      
        1 attcttttat gataggtcgt caggaacaac actgaccagc atatttagga ggagagcgta
       61 tatatttttc acgaagtttc atcaaactat acgacagtac agaagaattg tcatatttta
      121 aattataact ctaatgtaat aattcattat cccagaatgt atccgaacaa gaaataacgg
      181 ctcgttattc tttgttagat aaatgaagga gtacaaagaa aatggatgtt cctgttctga
      241 atttcgacca ctcacaccac aagctcaaaa ttcgcggcct gcaaagtccg gtggatgtgc
      301 tgaccttcga aggccgcgaa caactgagta ccccgttccg ctacgacatt cagtttacca
      361 gtaccgataa agccatcgcg ccggagtcgg tgctgatgca ggacggcgca ttcagcctga
      421 cggcaccacc ggtacaggga atgccggtgc agactgccct gcgcaccctg cacggcgtca
      481 ttaccagctt taaacacctc tcctcgtcac aggacgaagc ccgttacgaa gtgcgccttg
      541 aaccccgtat ggcgctgctt acccgcagcc gccagaacgc catctaccag aatctgacgg
      601 tgccgcaaat cgtcgaaaaa atcctgcgcg agcggcacca gatgcgcggt caggacttcg
      661 tgtttaacct caaaagcg
//
LOCUS       DAPQZD010000386          675 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPQZD010000386 DAPQZD010000000
VERSION     DAPQZD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.397"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(137..>675)
                     /locus_tag="RO454_005087"
     CDS             complement(137..>675)
                     /locus_tag="RO454_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HDY0936755.1"
                     /translation="PNSLPAEVTYVELLDTDGIYYQFRTLASTNASPGSVGQWSEKVS
                     GMSSVFNKAKNPPMSIHFCWDSVIDKKVYETWITLAGDVWKLMLTPYSPPGGGSEKYY
                     LRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKMEMCNGRSAYKHGYSY
                     PESTKNFIKDKKYPYGNW"
BASE COUNT          175 a          151 c          122 g          227 t
ORIGIN      
        1 aaaaaaaatt gaaataccat tcatcatagg gtgcaagcag tgatattttt tctttcgctg
       61 ctgtctggac tgatccggct atcattaacg caatggttaa tctataaatt attttagctg
      121 ttattttcat tgttacttac cagttaccat agggatattt tttatctttg ataaagtttt
      181 ttgtactttc cggataagaa tacccatgtt tataggcact tcggccgtta cacatttcca
      241 ttttttcacc tgaaaccgtt tctatcagaa tctgcttatc cgtcaggcga atattgggct
      301 tgtcaggttt ctccagccac actcctattt tcccttccgg agccagcccg attaacagat
      361 accgcagata atacttttca ctgccaccgc ctggtggtga ataaggtgtg agcatcaatt
      421 tccagacgtc tcccgccagt gttatccagg tttcatacac ttttttatca atcactgaat
      481 cccagcaaaa atggatagac attggtgggt ttttggcttt attaaaaact gacgacatcc
      541 ctgatacttt ctcgctccat tgtccaacac ttcctggact ggcattagta ctcgccagag
      601 ttctgaactg ataatagatg ccatcggtat ctaataactc cacataggta acttctgctg
      661 gtagggaatt agggt
//
LOCUS       DAPQZD010000387          675 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPQZD010000387 DAPQZD010000000
VERSION     DAPQZD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.398"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>675
                     /locus_tag="RO454_005088"
     CDS             <1..>675
                     /locus_tag="RO454_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDY0936756.1"
                     /translation="IVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKFVI
                     ERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTIAN
                     VRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEEGN
                     IVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALEDD
                     DDVQKV"
BASE COUNT          226 a          116 c          147 g          186 t
ORIGIN      
        1 atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg
       61 gtgtagaaat ctatgctgct gccaaacaag gtgaacccga tccagaatta aacacatctt
      121 taaaattcgt tattgaacgt gcaaaacaag cacaagttcc aaagcacgtt attgataaag
      181 caattgataa agccaaaggc ggcggagatg aaacgttcgt gcagggacgt tatgaaggct
      241 ttggtcctaa tggctcaatg attatcgctg agacattaac ttcaaatgtt aaccgtacga
      301 ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg
      361 tcagctatat gtttgacaat acgggtgtga ttgtatttaa aggaacagac cctgaccata
      421 tttttgaaat tttgcttgaa gctgaagttg atgttcgtga tgtaactgaa gaagaaggta
      481 atattgttat ttatactgaa cctactgacc ttcataaagg aatcgcggcg ctaaaagcag
      541 ctggaatcac tgagttctca acaacagaat tagaaatgat tgctcaatct gaagttgagc
      601 tttccccaga agatttagaa atctttgaag ggcttgttga tgcccttgaa gatgacgacg
      661 atgtacaaaa agttt
//
LOCUS       DAPQZD010000388          670 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPQZD010000388 DAPQZD010000000
VERSION     DAPQZD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.399"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>670
                     /locus_tag="RO454_005089"
     CDS             <1..>670
                     /locus_tag="RO454_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HDY0936757.1"
                     /translation="STKNWTHAIYFRFVIADYFISKVAKVLYLDADIICQGTIEPLIK
                     FSFPDDKVAMVVTEGQADWWEKRAHSLGVAGISKGYFNSGFLLINTAKWAAQQVSARA
                     IAMLNEPEVIKKITHPDQDVLNMLLADKLIFADIKYNTQFSLNYQLKESFINPVTNNT
                     IFIHYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTALLKPNNSNQLRYSAKHMLKK
                     HRYLK"
BASE COUNT          224 a          123 c          128 g          195 t
ORIGIN      
        1 agcacaaaga actggactca tgcaatatat tttcgttttg ttattgctga ttactttatt
       61 agtaaggtgg ctaaagtcct ttatctggat gcagatatca tttgtcaggg gactattgag
      121 ccactaatta aattctcatt tcctgacgat aaagtcgcaa tggttgttac agaagggcaa
      181 gctgactggt gggaaaaacg cgcccattcg ttaggtgttg cgggaatttc taaaggttac
      241 tttaactccg gttttttatt gattaatact gccaaatggg cagctcagca ggtttctgca
      301 cgagccattg caatgctaaa tgagccagaa gtaatcaaaa aaataacaca tcctgatcag
      361 gatgtattaa atatgttgct ggcagataaa cttattttcg ctgatattaa atataacacc
      421 cagtttagct taaattatca actaaaagaa agctttataa acccagtaac caacaatact
      481 atttttatcc attatatcgg gccaaccaag ccctggcatg attgggcctg ggattatcct
      541 gtatcacaag cctttatgga ggcaaaaaat gcttcgccat ggaagaatac agcgttgctg
      601 aaaccgaaca atagcaatca attaagatat agcgcaaaac atatgcttaa aaaacataga
      661 tatctaaaag
//
LOCUS       DAPQZD010000389          668 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPQZD010000389 DAPQZD010000000
VERSION     DAPQZD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.400"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            39..602
                     /gene="fimA"
                     /locus_tag="RO454_005090"
     CDS             39..602
                     /gene="fimA"
                     /locus_tag="RO454_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDY0936758.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          167 a          134 c          167 g          200 t
ORIGIN      
        1 tctttattca gctacttaaa tataaatttt ggagaattat gaaactcaaa catgtcggta
       61 ttattgtcgt ttctgtgttg gcgatgtcgt ctgctgcggt aagcgcagcc gagggtgatg
      121 aatcagtaac gaccactgtt aatggcggtg taattcattt taaaggtgaa gtggtaaatg
      181 ccgcttgtgc gattgattca gaatcaatga accaaacggt tgagctgggt caggttcgtt
      241 cttctcgcct ggctaaagcg ggtgacctca gctccgccgt tggcttcaat atcaagctga
      301 atgattgtga taccaatgtt tccagtaatg cagctgttgc attcctgggt actactgtca
      361 ccagtaatga cgatacatta gcgctgcaga gttcagcggc aggctctgcc caaaatgtcg
      421 gtatccaaat tttggaccgt acaggggagg tattagtact tgatggtgct acttttagcg
      481 ctaaaactga cttgattgat ggcacgaata tactgccgtt ccaggctcgt tatattgctc
      541 tcgggcagtc agtagctggt actgcaaatg ctgatgcgac cttcaaagtt caatatctat
      601 aattcactct taaggtgact atgcgtcatc tgaaattgaa acagggtgtg tgtggcatgg
      661 ctacgcac
//
LOCUS       DAPQZD010000390          654 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPQZD010000390 DAPQZD010000000
VERSION     DAPQZD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.401"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>654
                     /locus_tag="RO454_005091"
     CDS             <1..>654
                     /locus_tag="RO454_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDY0936759.1"
                     /translation="LKMLTLCKHLRDLQFDIVLDPFETIPSFKHSLILSSLKDSYILG
                     FDQWYKRYYSFYHPHDECLKEHMSTRATEILKHIYGEGEFSTDYDLHLPVDVEDKIKE
                     FIGDTRIVIINPLGAKKICRLTFEQIKVIYQEMKLHFENYRVIFTGLPQDLLTIPIPE
                     IETLPFNEFIYTVALTKYSDFVISVDTALVHIAAAYHKPTLAFYPNSRTPEYPSHLI"
BASE COUNT          218 a          118 c          103 g          215 t
ORIGIN      
        1 tccttaaaat gcttactcta tgtaagcatc tgcgggattt gcagttcgat attgtattag
       61 acccctttga aaccataccc tcatttaagc atagtttaat attatcaagt ttaaaagatt
      121 cttacattct gggttttgat caatggtata aacgttacta ttcattttac catccccacg
      181 atgaatgttt aaaagaacat atgagtacac gagcaacaga aatactgaag catatttatg
      241 gtgagggtga attctccacc gactacgact tgcatttacc agtagatgtt gaagataaaa
      301 taaaagaatt tattggagat acaagaatag tcattattaa cccattaggt gcaaaaaaaa
      361 tatgccgact tacgtttgag caaataaaag ttatatatca agaaatgaaa ctacactttg
      421 aaaattatcg ggtgatattt accgggttac cgcaagattt attgacaata ccaatacctg
      481 aaatcgaaac actaccattt aatgagttta tttataccgt tgcgttgacc aagtatagtg
      541 attttgtcat ttctgttgat acagctcttg ttcatatcgc tgcggcttat cataaaccaa
      601 cgcttgcatt ttatcctaat tcacgtactc cggaatatcc ctcacattta attt
//
LOCUS       DAPQZD010000391          652 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPQZD010000391 DAPQZD010000000
VERSION     DAPQZD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.402"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..652
                     /locus_tag="RO454_005092"
     rRNA            <1..652
                     /locus_tag="RO454_005092"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          164 a          155 c          194 g          139 t
ORIGIN      
        1 gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag cggtggagca
       61 tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt cttgacatcc acggaagttt
      121 tcagagatga gaatgtgcct tcgggaaccg tgagacaggt gctgcatggc tgtcgtcagc
      181 tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc ctttgttgcc
      241 agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga aggtggggat
      301 gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa tggcgcatac
      361 aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta gtccggattg
      421 gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc agaatgccac
      481 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag tgggttgcaa
      541 aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc atgactgggg
      601 tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc tt
//
LOCUS       DAPQZD010000392          628 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPQZD010000392 DAPQZD010000000
VERSION     DAPQZD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.403"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            241..498
                     /gene="repA"
                     /locus_tag="RO454_005093"
     CDS             241..498
                     /gene="repA"
                     /locus_tag="RO454_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDY0936760.1"
                     /translation="MSQTENAVTSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
BASE COUNT          174 a          129 c          162 g          163 t
ORIGIN      
        1 ggtatttcag gggcagacac ccgacatgcc agaaacagcc ggtcccgccc ggggccggca
       61 tcctggttaa ggcatttcct gcttttcagt catttcatta tcaaaatcac attaaacggt
      121 tgtaatcaga catgatttgt gcgccaacac cgatcatcgt cacaactttc aagtcgctga
      181 tttcaaaaaa ctgtagtatc ctctgcgaaa cgatctctgt ttgattattg aggaggcgag
      241 atgtcgcaga cagaaaatgc agtgacttcc tcatcgggaa aaaaacgacc ttacagaagg
      301 ggtaatcctg ttcctgcgag agaacgacaa aaagcgtctc ttgcaagaag gagtgccact
      361 cataaagcgt ttcatgccgt cattcagctt cgactgaaag aaaagttaag tgagcttgct
      421 gatgaagacg ggatcactca ggcacagatg cttgagtggc tgatagagtc agaggttaag
      481 cgcagaaaat ctttgtgagt atttgcgttt cttgctgttt cagtgatgag atgttagatt
      541 gctgatcgtt ttaaggaatt ttgtggctgg ccacgccgta aggtggcagg gaactggttc
      601 tgatgtggat ttacaggagc cagaaaag
//
LOCUS       DAPQZD010000393          624 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPQZD010000393 DAPQZD010000000
VERSION     DAPQZD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.404"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            49..285
                     /locus_tag="RO454_005094"
     CDS             49..285
                     /locus_tag="RO454_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDY0936761.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            328..600
                     /locus_tag="RO454_005095"
     CDS             328..600
                     /locus_tag="RO454_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDY0936762.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKNMFTGKKINRIH"
BASE COUNT          216 a          105 c          132 g          171 t
ORIGIN      
        1 attgttacct cacttaacat tgattaacat ttttaacaga ggcgtagcat gcatcaaaat
       61 tcagtgactt cagattctgc aggagcaatc actcgttact ttgcaaaagc taacttgcct
      121 actcagcagg aaactctcgg ggaaattgtg actgagattt tgaaagatgg acgtaatctg
      181 agtcgaaagt cgctttgtgc caaacttctt tgccgactgg aacaagcgac aggggaagag
      241 gaacagaaac attataatgc actaattggg ttgctttttg aataatgcaa cagcctgatt
      301 atttaacagg ttagctatga agtcgttatg aagacatctg ataatgaaag tattaaatat
      361 gaaattactg gccaggcggt gctccagata ctgcgcatga agataaattt ttcgttgcag
      421 actcttatta agcaattact cgtaatgaaa tcggctgagg aaaatacttt ccgacgagat
      481 ttaattgaca gcataattcg tgattttagc aacagtgata caggagggcc aaaccgaaga
      541 acagcgatcg ctgacaataa aaatatgttc actggtaaga aaataaacag aatacattaa
      601 aatttcataa gtaagatgag aggt
//
LOCUS       DAPQZD010000394          606 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPQZD010000394 DAPQZD010000000
VERSION     DAPQZD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.405"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..138)
                     /locus_tag="RO454_005096"
     CDS             complement(<1..138)
                     /locus_tag="RO454_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDY0936763.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
     gene            complement(382..564)
                     /locus_tag="RO454_005097"
     CDS             complement(382..564)
                     /locus_tag="RO454_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936764.1"
                     /translation="MMARKFGKQLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSH
                     TKSELTGGKRRYRIKN"
BASE COUNT          167 a          157 c          101 g          181 t
ORIGIN      
        1 ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca gttcacataa
       61 ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga gcaacccgat
      121 aagggcatat ttcgtcataa actttgacct ccctgcaagg aagaggctac aatcagtagt
      181 gttaaggtac ttcatgcaga cctcacgagt taatggatta acgagtgggg tcttcgcatt
      241 tctggtacct gcgtcttgcc tgaaccagaa aggctcagac acccgccgcg ataataccac
      301 attccctgtt gctgactatt accttcattt cccgcttttt actaccgaaa ctccagccaa
      361 taagtatgct ttttatttat atcaattttt aatccggtac cgacgtttcc ctccagttaa
      421 ctcgcttttc gtatgactca tggcaataaa attttcttta ccatatgaca acatcccaat
      481 aatatcaaaa ttgtctttta atttgatggc atccttttca cttacagcga caataagttg
      541 tttaccaaat tttctggcca tcattgcaaa ctgtgatact gctgatccac cacctaaatc
      601 aggcca
//
LOCUS       DAPQZD010000395          601 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPQZD010000395 DAPQZD010000000
VERSION     DAPQZD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.406"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..537
                     /locus_tag="RO454_005098"
     CDS             <1..537
                     /locus_tag="RO454_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936765.1"
                     /translation="KPLNNIYAWILAFMPFFFGVSPEEYTNYAIIIGGVTTIGLLVAD
                     RMALSESGCEPPSILWGVFLLPVYLWKRATITKGSRVPFIIIVASLAWVIFIGIPHKD
                     EVALEEAACPLVTTILKENNGSTAPKCMKVTIEDKVTDKFYRATATLDNGNDINITLE
                     LTGDRNFYVRVPNVYLNN"
BASE COUNT          163 a          130 c          137 g          171 t
ORIGIN      
        1 aaacctctga ataacattta tgcgtggatt ctagcgttca tgcctttctt ctttggagta
       61 tcgccggaag agtataccaa ttacgctatt ataattgggg gtgtaactac catcggtttg
      121 ttagtagctg acagaatggc tctatcggag tctggctgtg aaccaccttc aattctatgg
      181 ggggtattcc tcctgcctgt ctatctctgg aagcgcgcga ctattacaaa aggcagcaga
      241 gtgccattta tcataatcgt tgcgtcgctg gcttgggtta tcttcatcgg catcccacac
      301 aaggacgagg ttgctcttga agaagcagcc tgccctctcg tgacaactat tctgaaagag
      361 aataacggtt cgactgcacc aaaatgtatg aaggtgacaa tagaggacaa ggtgacggat
      421 aagttctata gagcaacggc tacactagac aatggtaatg acattaacat tactctggaa
      481 cttaccggcg acaggaactt ctacgtccgt gttccaaatg tttatctgaa caactaacgg
      541 ttgcaactca ttgataatgg cggtcacaat ttgattcttt tctataagaa tccgttgatc
      601 g
//
LOCUS       DAPQZD010000396          596 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPQZD010000396 DAPQZD010000000
VERSION     DAPQZD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.408"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>596
                     /gene="waaZ"
                     /locus_tag="RO454_005099"
     CDS             <1..>596
                     /gene="waaZ"
                     /locus_tag="RO454_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HDY0936766.1"
                     /translation="LSGPTSQKTPLSVLRTKDIIAVNGSVQYLLSHNIVPFIYVLTDV
                     RFLHQRRDDFYKFSQRSRYTIVNVDVYEHASKEDKLYILQNCLVLRSFYRREKGGFIK
                     KIKFNILSQIHKELLISVPLSKKGRLVGFCKDISFGYCSCHTIAFAAIQIAYSLKYAR
                     IICSGLDLTGNCSRFYDENNNPMPSELSKDLFKILPFF"
BASE COUNT          171 a          109 c          106 g          210 t
ORIGIN      
        1 tctttcgggc cctacatcgc aaaaaacacc tttgtcagta ttacgaacaa aggatattat
       61 tgctgtcaat ggttctgtgc aatatctgtt aagtcataat atcgttcctt ttatatatgt
      121 acttacagat gtccgctttt tacatcagcg tcgtgatgat ttttataaat ttagccagag
      181 aagccgttat accatagtaa acgttgacgt ttatgaacat gcttccaaag aagataaact
      241 ttatatcctc cagaactgcc tcgtattacg gtccttttat cgccgggaaa aaggtggttt
      301 tataaagaag attaaattta atatattgag tcagattcac aaagaactgc tgatctctgt
      361 accgttgtct aaaaaaggtc gtctggttgg attttgcaag gacattagtt ttggttattg
      421 ttcatgccat actattgcct ttgctgcaat tcaaatagca tattcactta agtatgcacg
      481 gattatttgt tctggtcttg atttaacggg taactgctct cgtttctatg acgaaaataa
      541 taatcccatg ccctcggaat tgagtaagga tttattcaaa atcttgccat ttttcc
//
LOCUS       DAPQZD010000397          585 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPQZD010000397 DAPQZD010000000
VERSION     DAPQZD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.409"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            38..>585
                     /gene="rnhA"
                     /locus_tag="RO454_005100"
     CDS             38..>585
                     /gene="rnhA"
                     /locus_tag="RO454_005100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDY0936767.1"
                     /translation="MTTKTPARSQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAA"
BASE COUNT          176 a          128 c          152 g          129 t
ORIGIN      
        1 aggaaaaatc ggcgcggcgc ttgattcagg agtttatatg acgacgaaga caccagcccg
       61 atcgcaagca aaaactcgca aaaaagacaa aaacaaaaat tctccccgca ccaatcccac
      121 aacgcctgtc gtagagttca atccccagct taaaaccgtg aaaatcttta gtgatggctc
      181 ttgccttaaa aatccgggtg gcccgggcgg ttacggtatc gttctccagt atcgtggtga
      241 ggaacgcgag ttctcagatg gttttcatag caccaccaat aaccgcatgg agatgatggg
      301 ggcacttatc gggctggagc gtttgaaata tccatgcaac gttattttgc actctgatag
      361 ccagtatctg aaaaacggca tgacacagtg gatgaaatgg tggaaacgca atggatggat
      421 gacttctgac aaaaaaccgg taaagaatgt tgatctgtgg aagcgtctgg atgaggccgc
      481 aagtcgacat aatgttcgct ggaagtgggt taaaggtcac gccgggcatc gtgaaaatga
      541 aatatgtgat cgactcgcga agatcgcagc tttttcagca gcaga
//
LOCUS       DAPQZD010000398          584 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPQZD010000398 DAPQZD010000000
VERSION     DAPQZD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.410"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>584
                     /gene="rfaJ"
                     /locus_tag="RO454_005101"
     CDS             <1..>584
                     /gene="rfaJ"
                     /locus_tag="RO454_005101"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase RfaJ"
                     /protein_id="HDY0936768.1"
                     /translation="RINTDKLQCLPCTQVWSRAMYFRLFAFQLLGLTLDRLLYLDADV
                     VCKGDISQLLHLDLNGAVAAVVKDVDPMQEKAASRLSDPELLGQYFNSGVVYLDLKKW
                     ANAKLTEKALSILMSKDNVYKYPDQDVMNVLLKGMTIFLPRGYNTIYTIKSELKDKTH
                     QNYKKLIIENTLLIHYTGATKPWHKWAIYPSTKY"
BASE COUNT          193 a           96 c          111 g          184 t
ORIGIN      
        1 tagaattaac actgataagc tccagtgctt gccttgtaca caggtctggt caagagcaat
       61 gtacttccgt ttatttgcat ttcaattatt gggtttaaca cttgatcgtt tgctttatct
      121 tgatgcagat gttgtttgta aaggcgatat tagccaacta ttgcatctgg atttaaacgg
      181 cgcggttgct gctgttgtta aagatgttga cccaatgcag gaaaaggcgg catccaggtt
      241 gtctgatcca gaattactcg ggcagtactt taattccggt gtcgtttatt tagatttaaa
      301 aaaatgggct aacgcaaaac taacagaaaa agcgttgtct attcttatga gtaaagataa
      361 tgtgtataaa taccctgatc aagatgttat gaatgtctta ctgaaaggga tgacaatatt
      421 tttgcctcgt ggatataata caatttatac aattaaaagt gaattaaaag acaaaacaca
      481 tcaaaactat aaaaagctga ttatagaaaa tactcttctt attcattata caggtgcaac
      541 taaaccgtgg cacaaatggg caatttatcc atcgacaaaa tact
//
LOCUS       DAPQZD010000399          581 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPQZD010000399 DAPQZD010000000
VERSION     DAPQZD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.411"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            60..>581
                     /locus_tag="RO454_005102"
     CDS             60..>581
                     /locus_tag="RO454_005102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936769.1"
                     /translation="MYNKPTSTSRLHSKLNVIKASMAKMSSDGEKTNSHGFAILCRNL
                     NERTLAPYTITLVNVSAFSSIERYFHSPQISNASQLPRIGQKIYDIYTHYQPSGFGAS
                     MSKIYNSGKAYLEKINCKPRIEVIDTWDVLQAHTHAPYMEMVHIETWGVFKEKLIEGL
                     NECYEDEYYTPDFD"
BASE COUNT          188 a           98 c          110 g          185 t
ORIGIN      
        1 cactcaagtt ctcatttcgt catcttggtg ggtttcatat atgtttgtat gatgatgaaa
       61 tgtataataa accgacttca acttctcgtt tacattcgaa attgaatgtt attaaagcca
      121 gcatggcaaa aatgtcctca gacggtgaaa agactaatag ccatgggttt gctattttat
      181 gtagaaattt gaatgagaga actttagcgc catatacgat tactctagtt aatgtttctg
      241 cgttttcttc catagagcga tactttcatt cgccacaaat atccaatgct tctcaactcc
      301 ctagaatagg acaaaaaatt tatgacattt acactcatta ccaaccatca ggttttgggg
      361 catcaatgtc gaaaatttac aattctggga aggcatattt agaaaaaatt aattgcaagc
      421 cgcggatcga ggttatagac acctgggatg tattgcaagc acatacacat gcgccttata
      481 tggaaatggt tcatattgaa acgtggggag tttttaaaga aaaattgatt gaaggtctaa
      541 atgaatgtta tgaggatgaa tattacacac ctgattttga c
//
LOCUS       DAPQZD010000400          577 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPQZD010000400 DAPQZD010000000
VERSION     DAPQZD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.412"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..569)
                     /locus_tag="RO454_005103"
     CDS             complement(<1..569)
                     /locus_tag="RO454_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HDY0936770.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVS"
BASE COUNT          234 a          133 c           80 g          130 t
ORIGIN      
        1 gaaacaatac caactattgc catcagaacc acagatataa taacactact tgaactattg
       61 gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc
      121 ggcaagaaaa aggttaccca ccagaaccct ttcaaagagc aattaaaact aaatcttacg
      181 ccgttgagac tagtcatcaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc
      241 aaaaccaaca gtgacaaagt caaaacacag tcaacaagcg gcatatctgc tgacactgtc
      301 attaagattg cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac attcccaccg
      361 gtaatcccat aagaaaatcg ttgtccgtta acttccatat tagcatagag ataacgctta
      421 catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa caacacattc
      481 accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga atgggacgga
      541 acatccattt cattaataac ttgagccata aaaattc
//
LOCUS       DAPQZD010000401          568 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPQZD010000401 DAPQZD010000000
VERSION     DAPQZD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.413"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>568
                     /locus_tag="RO454_005104"
     CDS             <1..>568
                     /locus_tag="RO454_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HDY0936771.1"
                     /translation="SFIHNGHAVSTGGVNENIFNGYFSDVELSKGNSALTEKVNRDYF
                     SKPADDYFLNNHIISYDPSKNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLR
                     SVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNG
                     EYWSHKGLDKAYSKEIYQLIKNDWKKVGS"
BASE COUNT          169 a           95 c          139 g          165 t
ORIGIN      
        1 ttcttttatc cataatggtc atgcggtatc tactggtggt gtaaatgaga atatttttaa
       61 tggatacttt tcagatgtcg agttatcaaa agggaattcc gcgctgacag agaaagtcaa
      121 cagggattat tttagtaaac ctgctgatga ttatttttta aataatcata taatttctta
      181 tgacccgtca aaaaaccagt ggaagaacct gggaacgact ccatttccgg gaactgctgg
      241 ttcatcggtg atttttgcag aacagcaaat ttatatactg ggaggggaga gaaaaccggg
      301 gcttcgttct gttagatcct ggacggggga gctttcgcat gatcgaatta aatggtctga
      361 acttccacca gtagcttcac ccgagggggt gtcaggagca tatgcctctg ttattgatgg
      421 aaatatcttt ctggccggtg gtgcatattt cccgggggcg gcagaaaagt atagcaatgg
      481 tgaatactgg tctcataaag gactggataa agcctattca aaagagatat atcagctaat
      541 aaaaaatgac tggaaaaaag ttggttct
//
LOCUS       DAPQZD010000402          561 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPQZD010000402 DAPQZD010000000
VERSION     DAPQZD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.414"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>561
                     /locus_tag="RO454_005105"
     rRNA            <1..>561
                     /locus_tag="RO454_005105"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          125 c          185 g          109 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga g
//
LOCUS       DAPQZD010000403          535 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPQZD010000403 DAPQZD010000000
VERSION     DAPQZD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.415"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>535
                     /locus_tag="RO454_005106"
     CDS             <1..>535
                     /locus_tag="RO454_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936772.1"
                     /translation="EVIQSWGRGLLERSGEQTKFVKEFQTTFNSALWELYLNEMFIRL
                     DYSVDYTKDSPDFCVTTPSGYQFNVEAVISDRPHNPPAGVLFSESKFKHQSTLKLIGK
                     IKDKHDLFTGVNGKKFPYSTMSHVVNKPFVLALAPFDSDLSLSQNNTIINRVLFGIEE
                     PTTRNLVTGSQTKVTSI"
BASE COUNT          163 a          114 c          106 g          152 t
ORIGIN      
        1 gagaagtgat acagtcgtgg gggcgtgggt tactggagcg tagcggtgag cagactaaat
       61 tcgttaaaga gtttcagaca accttcaatt ccgccttatg ggaattgtat ctgaacgaaa
      121 tgtttattcg tctcgattat tctgtcgact acacaaaaga cagtcccgat ttctgtgtta
      181 ctacaccgtc aggctaccag tttaacgtcg aagctgtgat atcagatcgg ccacataatc
      241 cacctgcagg tgttcttttt agcgaatcaa aattcaaaca tcaatcaacg ctaaaattaa
      301 tcggaaaaat caaagacaaa catgatcttt ttacaggagt taatggcaaa aaattcccgt
      361 actctacgat gtctcatgta gttaataagc cgtttgttct ggcgcttgca ccctttgact
      421 ccgatttatc attgtctcaa aataacacaa tcatcaatcg agtgttgttt ggcattgagg
      481 aacctaccac cagaaatttg gtgactggca gccaaactaa agttacctca atcaa
//
LOCUS       DAPQZD010000404          533 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPQZD010000404 DAPQZD010000000
VERSION     DAPQZD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.416"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>533
                     /locus_tag="RO454_005107"
     CDS             <1..>533
                     /locus_tag="RO454_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDY0936773.1"
                     /translation="PNHKAAKLSELDMMIVNSVPPGGNWKNIPLDVPSKRIEQIRDSY
                     AQGKGSRSTYYGRLLPDMPAYTINTYFNRPGNGCHIHYEQDRVLSQREAARLQSFPDD
                     FIFFGGQTAINTQIGNAVPPFLAFLIAKEIEKAIGNTGYYIDLFSGAGGLGLGFKWAG
                     WTPLLANDIEEKYLQTYS"
BASE COUNT          174 a           97 c          115 g          147 t
ORIGIN      
        1 cccaatcaca aggcagcaaa gctaagtgaa ttggatatga tgattgttaa ctcagtaccg
       61 ccaggtggca actggaaaaa tattccttta gatgtaccgt ctaaaagaat agagcaaatt
      121 agggatagtt atgcacaagg aaaaggaagc cgttcaacat actacggtag attacttcca
      181 gatatgcctg catatactat caatacatat tttaaccgac ctgggaatgg ttgccatatt
      241 cactatgagc aagatcgtgt tctatcccag agagaagctg caagactaca atcgttcccg
      301 gatgatttta ttttcttcgg aggtcaaaca gcaattaata cccaaatcgg taatgcagtg
      361 cccccatttt tagcttttct gattgcgaaa gaaatagaga aggcgatagg taatactggt
      421 tattatatcg atttattttc cggagctgga gggctgggac taggattcaa atgggctgga
      481 tggactccat tattggcgaa tgatattgaa gaaaaatatc tacaaacata ttc
//
LOCUS       DAPQZD010000405          524 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPQZD010000405 DAPQZD010000000
VERSION     DAPQZD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.417"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            97..345
                     /locus_tag="RO454_005108"
     CDS             97..345
                     /locus_tag="RO454_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936774.1"
                     /translation="MNNSIPERFIFQCALFKNLEKEVFMTHGYVDSHIIAHALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          151 a           94 c           97 g          182 t
ORIGIN      
        1 ataagtttat agaaaactca ttcatcttat ttttgtctgt cgctttagac tatacatagc
       61 ataattttat tgggtcacta ttcctgcgag gctgacatga ataattcaat cccagagaga
      121 tttatttttc aatgtgcttt gtttaaaaac ctcgagaagg aggtgttcat gacacatgga
      181 tatgttgata gtcatattat tgctcatgct ttacggttgc gtttaaaaga tgagaccagt
      241 gttattcttt ctgatctcta tctgcaaatc ttgcagtaca ttgaaatgca taagactaca
      301 ctaacggata tcattattaa tgacagggaa tccgtgctct cttaaaactt gagtaaggtt
      361 tttgaataaa catcagctgt atcactatgc tgatgcaaag tgagcatttc aggcgttatg
      421 ctttcttaat atatccgcaa tatcagaggg caaacaggaa tggagagcgc tcgttttcca
      481 ttcttgacgc ctgatatccc gcctaatttg tttaattctg ccta
//
LOCUS       DAPQZD010000406          524 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPQZD010000406 DAPQZD010000000
VERSION     DAPQZD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.418"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>524
                     /locus_tag="RO454_005109"
     CDS             <1..>524
                     /locus_tag="RO454_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936775.1"
                     /translation="LATQTYNEHYKYGTHTKADKANMQAATTKLAYFINNVVNAVEDE
                     KLCSVFYYAIKASKQAPEVFFRDAMTNSYSLEKLVYLVKSIKSGKCVYSIADMSGSRV
                     FALIDMINDEIDTFTNGAVFDLMNEAKKACEIKLDAGYTQANQLINLCERLGLVEKVK
                     GAGSAKAGTQQYRF"
BASE COUNT          175 a          105 c          103 g          141 t
ORIGIN      
        1 atctggcaac tcaaacatat aacgaacatt ataaatatgg tacgcacact aaagcagaca
       61 aagcaaatat gcaagctgcg accactaaac ttgcttactt catcaacaac gtcgtaaacg
      121 cagtagaaga cgaaaaatta tgctctgttt tctactatgc gattaaagca agcaaacaag
      181 cgccagaagt atttttccgt gatgcaatga ctaatagtta ttctctggaa aaactggttt
      241 atctggttaa atcaattaaa tctggcaaat gcgtttattc catcgctgat atgtcaggat
      301 ctcgtgtatt cgctttaatc gatatgatta acgacgagat cgacacgttc accaatggcg
      361 ctgttttcga tttaatgaat gaagctaaaa aagcgtgtga aattaaattg gacgctggct
      421 atactcaagc caaccagttg atcaatcttt gcgaacgtct cggacttgtt gaaaaagtca
      481 aaggagctgg gagcgccaaa gctggtacgc agcaatatcg cttc
//
LOCUS       DAPQZD010000407          520 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPQZD010000407 DAPQZD010000000
VERSION     DAPQZD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.419"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..275)
                     /gene="pdeL"
                     /locus_tag="RO454_005110"
     CDS             complement(<1..275)
                     /gene="pdeL"
                     /locus_tag="RO454_005110"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDY0936776.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATG"
BASE COUNT          166 a          116 c           92 g          146 t
ORIGIN      
        1 cccgtggcgg atatgatctc cggattgtac tgaaagaaaa tatcccgcca gaaagtaata
       61 tcgctctgaa tccccagttt ctcaaagagc tgtttctttt gatgtgaaat ggtctttgca
      121 ctgcgatttc tgtactgtga tatttctgtt acagacattc cctgcatcag cagcttgagt
      181 agcagtcgtt ctttctcgga tacgctacca ggcaatgaca aatgaaccgt tgtaccgaac
      241 tcttgcagaa aaacacgaaa atcacatgaa ttcatgaaca caccttttat cttttatcgc
      301 tgttcagtat gagtgattac ctgtggattc ctgagcacac ataaggggta accataaaca
      361 ttagcgatgc catacgtata acatcccaca tcactatcaa gaagattatc tttacctctg
      421 atgctcgctc aaaaacagtt acgctaaata ccttaaaaag gtaatcacaa gtcaaactca
      481 ggagataatc acgcaagaat gcaatattaa aattcagata
//
LOCUS       DAPQZD010000408          518 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPQZD010000408 DAPQZD010000000
VERSION     DAPQZD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.420"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..497
                     /locus_tag="RO454_005111"
     CDS             <1..497
                     /locus_tag="RO454_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936777.1"
                     /translation="IVLTDDDIIYPPDYVEKMLNFYNSFAIFNCIVGIHGCIYIDAFD
                     GDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYMDGSQRFVDVRFSRYML
                     ENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAIAGYSKLNLELVY
                     NVEG"
BASE COUNT          176 a           77 c           97 g          168 t
ORIGIN      
        1 tgatcgtact tacagatgat gatattattt accctcccga ttatgtagaa aaaatgctca
       61 atttttataa ttcctttgca atattcaatt gcattgttgg gattcatggc tgtatataca
      121 tagatgcatt tgatggagat cagtctaaaa gaaaagtatt ttcatttact caagggctat
      181 tgcgaccgag agttgtaaat caattaggta cagggactgt ttttcttaag gcagatcaat
      241 taccatcttt aaaatatatg gatggttctc aacgattcgt cgatgttaga ttttctcgct
      301 atatgttaga gaatgaaatt ggtatgatat gtgttcccag agaaaaaaac tggctaagag
      361 aggtctcatc aggttcaatg gaaggacttt ggaacacatt tacaaaaaaa tggcctttag
      421 acatcataaa agaaacacaa gcaatcgcag gatattcaaa acttaacctc gaattagtgt
      481 ataatgtgga agggtaaaaa cttacttttt tattcaca
//
LOCUS       DAPQZD010000409          517 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPQZD010000409 DAPQZD010000000
VERSION     DAPQZD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.421"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          165 a           96 c           83 g          173 t
ORIGIN      
        1 tagatacaat gtgataagct cctaagccct tgaagtaatc taaattgtct aaatgcttat
       61 ctgttaaacc tcgctgcgta gttcctttta aatgtttaaa aaaatctctt gcaaccggtt
      121 tactgttaaa aaggtaatta gatagtcgta atgatacacc atatgagaac attatataat
      181 gttctgcact tctgtttgct gaacttagat gataagtttt catatctggt tctctttaaa
      241 cttgggagaa ggttacgaca gtcgctctct ataaggactg aagcgaggtg gtcatttctt
      301 atattccgtt taaccttgac gataaaacgg acatatcaca tcaaccttag ccagatgact
      361 ccctccgata aaaacggata gtctgcctga tcatctccaa attgaatact cggcataatt
      421 atctaataaa tatcgtaaat ttatagaaat tgatttattg cattaactcg cgcaacatct
      481 tcaattaata cgtcttttgc gacaatagat aattgct
//
LOCUS       DAPQZD010000410          512 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPQZD010000410 DAPQZD010000000
VERSION     DAPQZD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.422"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            130..>512
                     /locus_tag="RO454_005112"
     CDS             130..>512
                     /locus_tag="RO454_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936778.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYETIMENNTNKESLCLQSEKGGKTSYKDITK
                     IQLATTEKQKLLASLEVVNSMSSYV"
BASE COUNT          173 a           79 c          101 g          159 t
ORIGIN      
        1 cttcatttga agtatgataa atagtcgcag tattttaatt taatttcata cttgcttatg
       61 aataatgcca gggaatagcc acattactat ttaattatct gagataaact caacgtcaag
      121 aggtgggtta tgattagata tagtggaata tttctatcgc tgttaatgct ttcaggttgt
      181 gcaaccgaag cattgaagaa tgaacaagct gaaactgtta ttactgcggg taacaaggcg
      241 gttgcggcga atgcaagttt ctttaaacaa tactatgctt ctcgtgaaaa ctttcttgtt
      301 aatttttatg cgactaatcc ggattgtgca ttgcgacctg attacgaaac gattatggaa
      361 aataatacta ataaagagtc cttatgtttg caatcagaga aagggggcaa aacatcgtat
      421 aaagacatta ctaaaattca attggcaaca acagaaaaac aaaaactact ggcttcactt
      481 gaggtcgtta acagtatgag ttcctatgtg ga
//
LOCUS       DAPQZD010000411          506 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPQZD010000411 DAPQZD010000000
VERSION     DAPQZD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.423"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..368)
                     /locus_tag="RO454_005113"
     CDS             complement(<1..368)
                     /locus_tag="RO454_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDY0936779.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     ANMVEDIREKVSVEMERFFP"
BASE COUNT          156 a          131 c           61 g          158 t
ORIGIN      
        1 tttggaaaaa acctttccat ctcaaccgac accttctctc tgatatcttc aaccatattg
       61 gcatattcaa acttcggttt tccggaaaca tgagggctaa gagactcaat acctaaaatc
      121 tttgccaccg ttgactgcgt ttttacaaca cactcaagaa gaagtttatt aaactctgcc
      181 tgattaaacg ctgattcttt tcgctccatc tgcgcttcat aaactcgcaa tcctaactca
      241 agcaacatag aggcaatact tgaataacta acatctttct cttttgctcc ctctgctcgc
      301 ctttgcacag caatagcatt tattttttca gacacatggt cattaacata aacctgaatt
      361 ttggccataa cacaccttat tcaccttcct aaacaaacaa tatattttcg tgctctctat
      421 tctgcacctt atgacgaatc gccattagat tcatcatact aaaatcacga ttcactatac
      481 gaatcagcaa tcatcaaatg acgaat
//
LOCUS       DAPQZD010000412          503 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPQZD010000412 DAPQZD010000000
VERSION     DAPQZD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.424"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(14..>503)
                     /locus_tag="RO454_005114"
     CDS             complement(14..>503)
                     /locus_tag="RO454_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936780.1"
                     /translation="SAVIFSTTGTFGKAVVQSGIGRYVRSTRYRAIDVNDFIANEGMK
                     NEGKHTQRIGPDHFITTYRHFLNNEVTGADIVVCHSTAHEETHFDGLHIYYNPFATVP
                     LDRNIFFPPEITHNFYDMEKEQSVQNHPDGALVSRQVFEPTRAHFRYIMKHWFPGFLK
                     DV"
BASE COUNT          145 a          106 c          101 g          151 t
ORIGIN      
        1 accagcagga tgcttaaaca tcttttagaa agcctggaaa ccaatgtttc atgatatacc
       61 taaaatgtgc tctagttggt tcaaaaacct gtctagatac cagcgctcca tcaggatgat
      121 tctgaacaga ctgttctttt tccatatcgt aaaaattatg agttatctct ggggggaaga
      181 aaatatttct gtccagtggc actgtcgcaa aaggattgta gtaaatatga aggccgtcaa
      241 agtgcgtctc ttcatgagca gtggaatgac aaactacaat atccgcacca gtgacttcgt
      301 tatttaaaaa atgccgatat gtagtgatga aatgatcagg tcctatcctt tgtgtgtgct
      361 ttccctcgtt tttcatccct tcattggcga tgaaatcatt cacatcaatg gctcgatatc
      421 gtgttgacct tacatatcta ccgataccgc tttgaaccac ggcttttcca aaagtgccgg
      481 tggtggagaa aataaccgca ctt
//
LOCUS       DAPQZD010000413          487 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPQZD010000413 DAPQZD010000000
VERSION     DAPQZD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.425"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>487
                     /locus_tag="RO454_005115"
     CDS             <1..>487
                     /locus_tag="RO454_005115"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDY0936781.1"
                     /translation="NLRYLDSGSAQSQITINDLANVEISAPSLNVQDLIVKSLKAFDD
                     KITTLSSMNQTLEQMSQTLFKSWFVDFDPVIDNALDAGNPIPEALQTRAKLRQKVRNS
                     ADFKPLPAEIRSLFPSEFEETELGWVPKGWKEGTLPEIAFINSTSWTNKNQPDYINYV
                     DL"
BASE COUNT          157 a          103 c           96 g          131 t
ORIGIN      
        1 aatttacgat atcttgatag cggttctgct caaagccaaa taaccatcaa tgatctagcg
       61 aatgtagaaa tatctgctcc ttcgctaaat gttcaagatt taatagtcaa gagtctcaaa
      121 gcttttgatg acaaaatcac aacactttca tccatgaacc aaaccttgga acaaatgtcc
      181 caaaccctgt tcaaatcctg gtttgtggat tttgatccag tgattgataa cgctctggat
      241 gcaggaaatc caatcccgga agccctgcaa actcgcgcca aattgcgtca aaaagtacgt
      301 aatagtgcag attttaaacc gcttccggcg gaaattcgct cgcttttccc aagtgaattt
      361 gaagaaacgg agttgggttg ggtgccaaag gggtggaaag aagggacgct tccagagata
      421 gcttttataa actccacatc gtggactaat aaaaatcaac cagattatat caattatgta
      481 gatctct
//
LOCUS       DAPQZD010000414          487 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPQZD010000414 DAPQZD010000000
VERSION     DAPQZD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.426"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>487
                     /locus_tag="RO454_005116"
     CDS             <1..>487
                     /locus_tag="RO454_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDY0936782.1"
                     /translation="FITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGKI
                     QFFYDDKATRMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLLQ
                     PLILGLWGDSGLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLLS
                     RY"
BASE COUNT          160 a           72 c          113 g          142 t
ORIGIN      
        1 tttcatcacc tttcaagatg cagatgatct ttctcatccg gagagaatac aaagacaagt
       61 tgaggttctt cgcaataata aggctgtaat ctgtatggct aactggatcc gtgttgcgtc
      121 aaatggaaaa attcaattct tctatgatga taaagccaca agaatgtctg ttgtatcgtc
      181 aatgataaaa aaagatattt ttgcgacagt tggtggctat agacaatctt taattggtgc
      241 agatacggag ttttatgaaa cagtaataat gcgttatggg cgagaaagta ttgtaagatt
      301 actgcagcca ttgatattgg ggttatgggg agactccgga cttaccagga ataaaggaac
      361 agaagctcta cctgatggat atatatcaca atctcgaaga gaatatagtg atatcgcggc
      421 aagacaacga gtgttaggga aaagtatcgt aagtgataaa gatgtacgtg gtttattatc
      481 tcgctat
//
LOCUS       DAPQZD010000415          482 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPQZD010000415 DAPQZD010000000
VERSION     DAPQZD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.427"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>482
                     /locus_tag="RO454_005117"
     CDS             <1..>482
                     /locus_tag="RO454_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDY0936783.1"
                     /translation="SDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGE
                     YLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIV
                     KDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVYA
                     "
BASE COUNT          159 a          104 c          104 g          115 t
ORIGIN      
        1 tccgacaaca aaaaaaccgt gttacccggg ctaacaacga ccagaaagcc cattgttaca
       61 aaaaaacttt cagaaatcgt tgccgaagag cttgaaagaa tgatccgcca ggaggagata
      121 agggaaggtg aatacctgcc ttctgaacgt gaacttatgg cattctttgg cgttggaaga
      181 ccatctgtca gagaggcttt atcatctctc agacataaag ggctaatccg gtataacaag
      241 ggagaaaaag ccagtgtctg ccgtccatca ccagaaacca tcattaatgg gttatcaggc
      301 attgttaaag attttttatc tacagctgat ggcatcaagg attttgaaag actacgcatc
      361 ttttttgaaa ccaaccttgt aagatatgca gcaaaaaatg caacgcctga ggatattcac
      421 aggcttgaga aggtgctcag tatccatcat aactcactga gaacccccga agtgtatgct
      481 ca
//
LOCUS       DAPQZD010000416          478 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPQZD010000416 DAPQZD010000000
VERSION     DAPQZD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.428"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            87..278
                     /locus_tag="RO454_005118"
     CDS             87..278
                     /locus_tag="RO454_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDY0936784.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          126 a          118 c           89 g          145 t
ORIGIN      
        1 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacggc
       61 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
      121 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
      181 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
      241 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct ccactctcac
      301 agaacataac ggcacctcgc tgtcggatgc ttttgctctt tgagattatc aaagcggcag
      361 atattctttc atcttaaatt ttacgtcttt gttctgactg cagttcggct gcgaaataaa
      421 tacatcatta atacatatgt tgtgatgatg tatttttata aattattccc cacatgaa
//
LOCUS       DAPQZD010000417          470 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPQZD010000417 DAPQZD010000000
VERSION     DAPQZD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.429"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          144 a          110 c           87 g          129 t
ORIGIN      
        1 ataagttatg acgaattctc accatccagt tactacctgt acatactaat caaaaaaaac
       61 gagtacgcaa gatacgaaag tacttttctt gttcaccagt tagcttacct tgatcctcac
      121 cagcgactgg aagataggta gcagtatatg tggaaacctt cccctagcta ggtctacaaa
      181 cgtagtttgg gcatttaggc tccacgattc acggtttgac acggcctgat atctgcacat
      241 tcgttttgat atgcagcaat cgtgaaaaag gaaaatatac gctcgacaga ccaaaacggc
      301 actacaccgc acccgcctgc ggttttcata tcactaaaaa ttttcagcct ggatttttta
      361 caaacgatac cgccagtccg tgccagtcct cgagatgtac tgcgatacag gaactgaaaa
      421 gaatgaaaag aaattcattt tttttcagtt ttactgtccg tcgaatctat
//
LOCUS       DAPQZD010000418          465 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPQZD010000418 DAPQZD010000000
VERSION     DAPQZD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.430"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>465
                     /locus_tag="RO454_005119"
     CDS             <1..>465
                     /locus_tag="RO454_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDY0936785.1"
                     /translation="AEKFAALKREQALPLAINPNSDQYLEERLQLLDEQLATVTRLAK
                     DNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRIKITELLMDVDDWTGFSR
                     HFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTYAKLSWLQAWH"
BASE COUNT           99 a          158 c          141 g           67 t
ORIGIN      
        1 cggccgagaa gttcgccgca ctcaagcgcg agcaggccct gcccctggcg atcaacccga
       61 acagcgacca gtacctggaa gagcgtttgc agctgctgga cgagcagttg gccaccgtca
      121 cccgcctggc caaggacaac gagctgcccg atgccatcct caccgagtca gggctgaaaa
      181 tcaccccgct ggatgcggcg gtgccggatc gggcgcaggc gctgatcgac caaaccagcc
      241 agttactgcc gcgcatcaag atcaccgaac tgctgatgga cgtggacgac tggacgggct
      301 tcagccgcca cttcacccac ttgaaggacg gggccgaggc caaagacagg acgttgctgc
      361 tgtccgcaat cctcggtgat gcgatcaacc tcgggctgac caagatggcc gagtcgagcc
      421 ccggcctgac ctacgccaag ctgtcctggc tgcaagcctg gcaca
//
LOCUS       DAPQZD010000419          462 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.431, whole genome shotgun sequence.
ACCESSION   DAPQZD010000419 DAPQZD010000000
VERSION     DAPQZD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.431"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          144 a           99 c           78 g          141 t
ORIGIN      
        1 gaaggtcctt tacttcacca ttcacatcca cacacagata aatcaatacg ttgtgatttc
       61 tgacttccct cccgtttcgg tagaaaaatc aacctctgtt cagtgatggc acaccataga
      121 ccatgagctg gataattgcg ttgtttacag cagccagcgc tttgatggca ctatatccct
      181 cacgcttctg aatataattc atagcgccgt gtaattatct ttaatctcaa gttagctaat
      241 ctcagcatat cgtgttgata tgcaatgtga aatacagaga acagtgacta cagctgatgg
      301 aggaagctat ttccgatatt cgtcaacttc caaatatatc gtttaaaaca ggtatcagat
      361 atgaaagcac tactaattac tgcattattc atgggattca gttagtttca ccaggcaaac
      421 attaaccata gctaatgttt atagccatat aaactattag ta
//
LOCUS       DAPQZD010000420          458 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.432, whole genome shotgun sequence.
ACCESSION   DAPQZD010000420 DAPQZD010000000
VERSION     DAPQZD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.432"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(311..>458)
                     /locus_tag="RO454_005120"
     CDS             complement(311..>458)
                     /locus_tag="RO454_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDY0936786.1"
                     /translation="SDLAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVVVSGA
                     TEPE"
BASE COUNT          110 a          135 c           90 g          123 t
ORIGIN      
        1 cataccccgt ctcccgttat ccggaagctg acaacaataa aaaagccacc agttaactac
       61 tgatggctct gataactcat gcaagcgtct cagacgatcc actgacacta ccggtgagtt
      121 taacgatacc ttccatttga ctggctcact ttttatgatg atgccggtac atttatctcc
      181 agcaccagac tttctatctc aacgccatac gttgcatttt tggtaatatc cgtcagcgtc
      241 agtgcattta gtcccactgc cagactgtcc tttatggcct agaatgccgg gccagtacga
      301 tgacgtagta tcactccggc tcagttgcac cactgaccac cacatcacct tctgctgcaa
      361 tcgcctgcat cagggtataa ggggttatgg ccaccggact accaaacggc tgccagccct
      421 ctttcagttt atgtgtcagc ttttccgcaa gatctgac
//
LOCUS       DAPQZD010000421          457 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.433, whole genome shotgun sequence.
ACCESSION   DAPQZD010000421 DAPQZD010000000
VERSION     DAPQZD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.433"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..293
                     /locus_tag="RO454_005121"
     CDS             <1..293
                     /locus_tag="RO454_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936787.1"
                     /translation="SYRDLEKKSYSGPFDLVSGVISPDAKYVDPFGLGCDIDKVNHSI
                     PHVYEISTAQRVAFKSDPALSAIINKEQIDKKCRQLLKASASLKDLGGYLSQ"
BASE COUNT          152 a           67 c           97 g          141 t
ORIGIN      
        1 gtagttacag agatttagaa aaaaaaagtt attcaggccc atttgatctc gtctcaggcg
       61 ttatctctcc tgatgcaaaa tatgtagatc ctttcggtct ggggtgtgat attgataagg
      121 tcaatcatag tataccacac gtgtatgaaa tatccacagc acaacgagtt gcatttaaaa
      181 gcgatccagc gttatctgcg ataataaata aagaacaaat agataaaaag tgtcggcagt
      241 tattaaaagc atctgcatct ttgaaagatt taggtggata tttaagtcag tagaatatgt
      301 caccgttact ctactgtgct ataggagata gcgaagaaga gttttttcaa tcaaatattg
      361 ccagagtttt attattggtt gaagaagact tgaagacgtt ggtgaacaat ttcaagaggc
      421 tattttttaa tgccaatggt attgctgcag gataagt
//
LOCUS       DAPQZD010000422          451 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.434, whole genome shotgun sequence.
ACCESSION   DAPQZD010000422 DAPQZD010000000
VERSION     DAPQZD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.434"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..374)
                     /locus_tag="RO454_005122"
     CDS             complement(<1..374)
                     /locus_tag="RO454_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDY0936788.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT          120 a           99 c          104 g          128 t
ORIGIN      
        1 ccaatggtgt tggtgccaac ggcctgacca tttttcggtg caggcgcatg ccacagatcg
       61 gcaaccatgt ttacgcaacg caggtcgatt gctgcagatt gcggatattc ttctttgtcg
      121 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
      181 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
      241 atctgaaatg caacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
      301 gtagaaatgg cctttgcacc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
      361 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
      421 atccgcaatg gaagcaaggc attacaggct t
//
LOCUS       DAPQZD010000423          450 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.435, whole genome shotgun sequence.
ACCESSION   DAPQZD010000423 DAPQZD010000000
VERSION     DAPQZD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.435"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          135 a           98 c           95 g          122 t
ORIGIN      
        1 gaatagtcag gggatattcc atagaactga acgttcatcc cccagaatga gaagttgaac
       61 atgcctccat agcgaatagt gttccggact cgaatcgttg aacgatatcc tgcaatttgg
      121 agcatgaggt gtttctatcc tatgcaacag aatcatcatt aatccagata taggcctgaa
      181 aaacaggcct ggcaatatcg ataccaacaa tcctgatcgt gttcatggga tgttactctc
      241 ggattaatgc agtcagtaaa agtatggcac tgactgcatt aagaagggac gtccatcata
      301 tcgtgacaca aacgctccca ccgacatcgc cattgagtcc tgcaccgtat cggtgaactt
      361 gtaactatcc cgtataatca ttctatacac atttattggc ttgatgagtc aaatacttct
      421 taatttcgga gagaagtgtg tcaggtgata
//
LOCUS       DAPQZD010000424          443 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.436, whole genome shotgun sequence.
ACCESSION   DAPQZD010000424 DAPQZD010000000
VERSION     DAPQZD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.436"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            84..158
                     /gene="tap"
                     /locus_tag="RO454_005123"
     CDS             84..158
                     /gene="tap"
                     /locus_tag="RO454_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDY0936789.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            151..>443
                     /locus_tag="RO454_005124"
     CDS             151..>443
                     /locus_tag="RO454_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDY0936790.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLA"
BASE COUNT           98 a          117 c          122 g          106 t
ORIGIN      
        1 caggtttggc gactaagaga agattaccgg ggcccactta aaccgtatag ccaacaattc
       61 agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcac
      121 tccttctgcg cattgtaagc gcaggatggt gtgactgatc ttcaacaaac gtattaccgc
      181 caggtaaaga acccgaatcc ggtgtttaca ccccgtgaag gtgcaggaac gctgaagttc
      241 tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgctattcat
      301 gtggcgcatg cccgttcgaa gggactgcgt cggcgcatgc caccggtgct gcgtcgacgg
      361 gctattgatg cgctgctgca ggggctgtgt ttccactatg acccgctggc caaccgtgtc
      421 cagtgctcca tcaccacgct ggc
//
LOCUS       DAPQZD010000425          438 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.437, whole genome shotgun sequence.
ACCESSION   DAPQZD010000425 DAPQZD010000000
VERSION     DAPQZD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.437"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(112..438)
                     /locus_tag="RO454_005125"
     CDS             complement(112..438)
                     /locus_tag="RO454_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936791.1"
                     /translation="MQRNAVRQVFKYRQQKIKGRMTTDKETNEAFANADTFYKSDKIF
                     DEKENIKIGTEYLQYWIDKEATIEEAYRTYRGTDEAYYSVIKPCAEKLAKDPDNIQIL
                     MEGIGR"
BASE COUNT          123 a           86 c           56 g          173 t
ORIGIN      
        1 caactgcaca ctttcatttt tttcaattga caggttttca cgattttaat cttatcgcct
       61 gaaaaacagg ggaaagaaaa aaaacaaagt aatattaaca taatgcgcat actatcttcc
      121 tattccctcc attagtattt gtatgttatc aggatcttta gccagttttt cagcgcaagg
      181 ttttataaca gaatagtatg cttcatctgt tccacgatat gtccgatatg cctcctcaat
      241 tgttgcttct ttatctatcc agtattgcaa atactctgtg ccaatcttaa tattttcttt
      301 ttcatcaaat attttatcag atttataaaa tgtatcagca ttagcgaaag cttcatttgt
      361 ctctttatca gtagtcattc ttccttttat tttttgttgt cgatatttaa aaacctgcct
      421 cactgcattt cgttgcat
//
LOCUS       DAPQZD010000426          438 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.438, whole genome shotgun sequence.
ACCESSION   DAPQZD010000426 DAPQZD010000000
VERSION     DAPQZD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.438"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..>438)
                     /locus_tag="RO454_005126"
     CDS             complement(<1..>438)
                     /locus_tag="RO454_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDY0936792.1"
                     /translation="QGFSTAGNARTMDDPRNSLFMHYKSLLNEIKPNGFIFENVAGLL
                     NMEKGKVFERVKEEFSSTMKTMNGWILNSEHYAIPQRRKRVILVGSNDPLFSIEPPQK
                     LTEDKESWVSVKDALSDLPPLQHGEDGSGKYYIHHPENDYQLFM"
BASE COUNT          131 a           82 c           71 g          154 t
ORIGIN      
        1 catgaataat tgatagtcat tttcggggtg atggatataa tatttaccac ttccatcttc
       61 accatgttgc aaaggaggca gatcagataa tgcatccttt acagataccc aagattcctt
      121 atcttctgtt aatttttgtg gaggttcaat tgaaaataaa gggtcgtttg aaccaaccaa
      181 tataaccctt tttcggcgct gagggattgc ataatgttca gagtttaata tccatccatt
      241 catagttttc atggttgatg agaattcttc tttgactctt tcaaagacct tacctttttc
      301 catattcaac aaaccagcaa cattttcaaa aataaaaccg tttggtttta tctcatttaa
      361 gagagattta taatgcataa aaagtgaatt tcttggatca tccattgttc tggcatttcc
      421 tgccgtagaa aatccctg
//
LOCUS       DAPQZD010000427          434 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.439, whole genome shotgun sequence.
ACCESSION   DAPQZD010000427 DAPQZD010000000
VERSION     DAPQZD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.439"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..374)
                     /locus_tag="RO454_005127"
     CDS             complement(<1..374)
                     /locus_tag="RO454_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDY0936793.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIG"
BASE COUNT          118 a           92 c           95 g          129 t
ORIGIN      
        1 ccaatggtgt tggtgccaac ggcctgacca tttttcggtg caggcgcatg ccacagatcg
       61 gcaaccatgt ttacgcaacg cagatcgatt gctgcagatt gcggatattc ttctttgtcg
      121 atccagtttt tgttaatgga taaatccatc aacttatgga cattttcgtc gtcccaggtc
      181 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc gtcattgata
      241 atctggaatg cgacatcatc acgcatttcg tgcagcggaa aacgttttga ttctgcgata
      301 gtggaaatag acttcgcacc aaaacgtgaa tcgagtagtt ccgaccttaa atccgttact
      361 tgcttcttat ccattttaaa ctccttaaaa tgattggatc gcattaaaaa agtaggattt
      421 atcgataaag taag
//
LOCUS       DAPQZD010000428          418 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.440, whole genome shotgun sequence.
ACCESSION   DAPQZD010000428 DAPQZD010000000
VERSION     DAPQZD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.440"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..>418)
                     /locus_tag="RO454_005128"
     CDS             complement(<1..>418)
                     /locus_tag="RO454_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDY0936794.1"
                     /translation="NDLNSYTNANSVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAE
                     NMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIISILFLYFSLFYTAYKRRA
                     LGLLILTLGIVGIGLSDVIIWARSIPIIVISAIVLLL"
BASE COUNT          136 a           86 c           73 g          123 t
ORIGIN      
        1 gagtaagagg actatagcgg atataacgat aattggaatg ctgcgtgccc atatgatcac
       61 atcactgagt ccaatcccca caatgccaag cgttaatatc aacaaaccca aagctcgtct
      121 tttatatgct gtataaaaca gtgaaaaata gaggaaaagg atggaaataa ttcccatcag
      181 acctttcagt gaccctgctt caattatctc attatgtaga tgtacgttag aaaactccaa
      241 tgcccctctt agcctgttgt gttctgcaac taacaaattc atattttcag cgcgtgactc
      301 tgctgatcta aatgaaaaag gtgactttat gaatatattt aaaccaattt cgtacattgc
      361 cagtcttgca cctagggaag tcacgctatt agcattggta taactgttta aatcattt
//
LOCUS       DAPQZD010000429          415 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.441, whole genome shotgun sequence.
ACCESSION   DAPQZD010000429 DAPQZD010000000
VERSION     DAPQZD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.441"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..249
                     /locus_tag="RO454_005129"
     CDS             <1..249
                     /locus_tag="RO454_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296213.1"
                     /note="WbbJ; catalyzes the transfer of the O-acetyl moiety
                     to the O antigen; part of the lipopolysaccharide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDY0936795.1"
                     /translation="IHSSPTIIPSSRPLESAPVYIGERVWIGENVTILPGACIGNGVV
                     IGANSVVRGEIPNNVIIAGVPAKIVKKYNYERMQWERI"
BASE COUNT          122 a           67 c           94 g          132 t
ORIGIN      
        1 atccatagtt caccaactat tattccttcg tctaggcccc ttgaatctgc acctgtgtat
       61 attggagagc gtgtgtggat tggcgaaaat gtgacaatat taccaggtgc gtgtataggt
      121 aatggtgtag ttattggcgc aaacagtgtt gttcgtggtg agattcctaa taatgtgatc
      181 attgctggtg ttccagctaa aattgttaaa aaatataact atgagcgtat gcaatgggaa
      241 agaatatagt tgtaatatcg gctgttaatt ttacaaccgg aggccccttt accgtactaa
      301 aaaatgtgct tacagcaact aaagatagag ccgaatgtaa atttattgca ctggttcata
      361 gctctgctga actaatggaa ttatttccgt gggttgaatt tatagagtat ccaga
//
LOCUS       DAPQZD010000430          410 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.442, whole genome shotgun sequence.
ACCESSION   DAPQZD010000430 DAPQZD010000000
VERSION     DAPQZD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.442"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(66..222)
                     /locus_tag="RO454_005130"
                     /pseudo
     CDS             complement(66..222)
                     /locus_tag="RO454_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           83 c           81 g          127 t
ORIGIN      
        1 tagagtgtga ttacaatctc cggcaatgac actgttagtg caacagtaat caggcctccc
       61 tcttagtatt gcttacgctg aaagaataac tggcagcctg ccacaccatg ttgtctggca
      121 acgaggaaga ccgccatttt cggttcaagg ctctgctgaa aaatagcgat tttcctgtgt
      181 ggcacgctgt ctgcgtttct ctgcttctaa gacaccaatt atctattctc caactactag
      241 tctaaaagac tagtatcact actatcagtt atttaaatga tattgattgc ctagagattc
      301 agggagctag tctagttatt cagagaacgg tatcttaaca attttgttat gattttagga
      361 gatctggatt tctattcatg atgcttggcg acaaaaataa atgaacaaaa
//
LOCUS       DAPQZD010000431          405 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.443, whole genome shotgun sequence.
ACCESSION   DAPQZD010000431 DAPQZD010000000
VERSION     DAPQZD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.443"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>405
                     /locus_tag="RO454_005131"
     CDS             <1..>405
                     /locus_tag="RO454_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HDY0936796.1"
                     /translation="QSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGI
                     YLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQ
                     TLELVFTAGSATVTPKLNGVAGPAFQVIKDSLT"
BASE COUNT          101 a          105 c          116 g           83 t
ORIGIN      
        1 cagagcaata aagcctggtc cctgacgcgc ccggtggatg acgcagtctc cctgctgaca
       61 cggggtggca gactgagctg taagtttcga ctgtcaggcg cactgaccaa caatcagttc
      121 ggtctgggaa tttatctgta taccgatgta gcgttacctg acgtcgtggc gatgaccggg
      181 actggtaacc cgttcctgat gtcgttcttc acccagacca cagacggcaa actgaatctg
      241 atgcatcaca agaaagcagg aaacacaaag ttgggcgagt tcgggaatta cagtaacgac
      301 tggcagacgc tggagctggt gttcaccgcc ggcagtgcca cggttactcc gaaactgaat
      361 ggagtggctg gcccggcatt ccaggtcata aaagacagtc tgaca
//
LOCUS       DAPQZD010000432          399 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.444, whole genome shotgun sequence.
ACCESSION   DAPQZD010000432 DAPQZD010000000
VERSION     DAPQZD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.444"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(32..>399)
                     /locus_tag="RO454_005132"
     CDS             complement(32..>399)
                     /locus_tag="RO454_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDY0936797.1"
                     /translation="AAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASA
                     FYYGDPLGEMRLVSRVFEM"
BASE COUNT           85 a          104 c           98 g          112 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagctt
//
LOCUS       DAPQZD010000433          395 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.446, whole genome shotgun sequence.
ACCESSION   DAPQZD010000433 DAPQZD010000000
VERSION     DAPQZD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.446"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..268)
                     /locus_tag="RO454_005133"
     CDS             complement(<1..268)
                     /locus_tag="RO454_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HDY0936798.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAI"
BASE COUNT          117 a           90 c           53 g          135 t
ORIGIN      
        1 gaatcgcaag gtataacatc attccatttg taatagccac aaaaagcagg aaccccatca
       61 ttccactagc acatatatca aagccaacat ccctgacatc acttttaacc gatactaaac
      121 gcagaaaagt tggccataat aaaacccata aaaacacata agttcccggc tttatgactt
      181 gtaataatga tgggatatta acaccctcta cagagagtac ctcctttagt ttttgcatct
      241 ccatttttat atcatagtga gtgatcataa tggccccttc aatcgttctt tcttatcatc
      301 caccatctgt tttaacgcat cttcagacgg tgttggtatt aatttctgtc gtttattttc
      361 ttcattatat ctgttgtttt gttgaatttc ttcct
//
LOCUS       DAPQZD010000434          384 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.447, whole genome shotgun sequence.
ACCESSION   DAPQZD010000434 DAPQZD010000000
VERSION     DAPQZD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.447"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            48..124
                     /locus_tag="RO454_005134"
     tRNA            48..124
                     /locus_tag="RO454_005134"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="RO454_005135"
     tRNA            167..242
                     /locus_tag="RO454_005135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT          107 a           83 c           92 g          102 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaatactt cagagtgtac ctgcaaaggt tcactgcgaa
      301 gttttgctct ttaaaaatct ggatcaagct gaaaattgaa acactgaaca acgaaagttg
      361 ttcgtgagtc tctcaaattt tcgc
//
LOCUS       DAPQZD010000435          377 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.448, whole genome shotgun sequence.
ACCESSION   DAPQZD010000435 DAPQZD010000000
VERSION     DAPQZD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.448"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..366
                     /locus_tag="RO454_005136"
     CDS             <1..366
                     /locus_tag="RO454_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0936799.1"
                     /translation="TEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADR
                     GLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIK
                     LSLGGLSPVEYRTEYQKAG"
BASE COUNT          124 a           72 c          106 g           75 t
ORIGIN      
        1 acagaggagc tggtgaagcg gatgttaaat aaggggctgg aaagccttgc tgaaggggag
       61 aaaccgctgc tccacagtga tcaaggatgg cattaccgga taaaaagtta tcagtccgcc
      121 ctggcggaca gagggttagt gcagagcatg tcgcgcaagg gcaactgcct tgataatgcg
      181 gtaatggaga acttctttgg tcacctgaaa gaagaaatgt actatcgccg ggactacaga
      241 aatgtagaag agctggaaaa tgccgttaac gaatacataa cttactggaa tcaaaaaaga
      301 ataaaactca gtcttggggg actcagcccg gtagaatacc ggactgagta tcaaaaagcc
      361 ggttaactga aactgtc
//
LOCUS       DAPQZD010000436          373 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.449, whole genome shotgun sequence.
ACCESSION   DAPQZD010000436 DAPQZD010000000
VERSION     DAPQZD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.449"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            9..>373
                     /locus_tag="RO454_005137"
     CDS             9..>373
                     /locus_tag="RO454_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068264.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDY0936800.1"
                     /translation="MTVSGAGYVGLSNGILMAQNHEVVAFDTHQKKVDLLNDKLSPIE
                     DKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDTSSVEAVIRDV
                     TEINPNAIMVVKSTVPVGFT"
BASE COUNT          131 a           71 c           61 g          110 t
ORIGIN      
        1 cactaaaaat aactgtttca ggcgctggtt acgttgggct ttcaaatgga attctaatgg
       61 ctcaaaatca tgaagtggtt gcatttgata cccatcaaaa aaaagttgac ttacttaatg
      121 ataaactctc tcctatagag gataaggaaa ttgaaaatta tctttcaact aaaatactta
      181 attttcgcgc aactactaac aaatatgaag cctataaaaa tgccaattac gttattattg
      241 ctacaccaac gaattatgac ccaggttcaa attactttga tacatcaagc gttgaagctg
      301 tcattcgtga cgtaacggaa atcaacccaa acgcaattat ggtggttaaa tctacggtcc
      361 cagtaggttt cac
//
LOCUS       DAPQZD010000437          371 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.450, whole genome shotgun sequence.
ACCESSION   DAPQZD010000437 DAPQZD010000000
VERSION     DAPQZD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.450"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..>371)
                     /locus_tag="RO454_005138"
     CDS             complement(<1..>371)
                     /locus_tag="RO454_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936801.1"
                     /translation="YKFQIYETKRMIGLLDMPENKTSFGSMGIQIRTAAITLGLDPSE
                     LTTRNQLELATCLMEDDFTFQIAATYLRDLVLFDYPSSATLYMTNEQYIMAGIRYNRG
                     VERDLGFFICLINNLPARDTD"
BASE COUNT          127 a           72 c           56 g          116 t
ORIGIN      
        1 atctgtatca cgcgcaggca aattattaat taaacaaata aaaaaaccca aatctcgttc
       61 aacaccacga ttatatctaa taccagccat aatatactgc tcattcgtca tatacagtgt
      121 tgccgaagag ggataatcaa aaagaacaag atctcttaaa taagtagcag caatttgaaa
      181 ggtaaaatca tcctccatta ggcatgtcgc taactctaac tgatttctgg tagttaattc
      241 tgaagggtct agacctagag taatcgctgc ggttcgtatt tgtataccca ttgagccaaa
      301 tgatgtttta ttttcaggca tatctaacaa tccgatcatt ctttttgttt catatatctg
      361 aaacttataa t
//
LOCUS       DAPQZD010000438          362 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.451, whole genome shotgun sequence.
ACCESSION   DAPQZD010000438 DAPQZD010000000
VERSION     DAPQZD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.451"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>362
                     /locus_tag="RO454_005139"
     rRNA            <1..>362
                     /locus_tag="RO454_005139"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           96 a           87 c          114 g           65 t
ORIGIN      
        1 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
       61 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      121 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      181 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
      241 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
      301 cggggctaaa ccatgcaccg aagctgcggc agcgacacta tgtgttgttg ggtaggggag
      361 cg
//
LOCUS       DAPQZD010000439          358 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.452, whole genome shotgun sequence.
ACCESSION   DAPQZD010000439 DAPQZD010000000
VERSION     DAPQZD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.452"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>358
                     /locus_tag="RO454_005140"
     CDS             <1..>358
                     /locus_tag="RO454_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDY0936802.1"
                     /translation="SNNQTSFTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSS
                     ASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKP
                     DDLLITLESIPVSQLPA"
BASE COUNT          101 a           73 c           83 g          101 t
ORIGIN      
        1 tcaaataatc agaccagttt caccagtggt tattcggtaa cggtgacacc tgcggccagt
       61 aatgcaaaag tgaatgtttc tgcggggggc ggtggttcag tgatgattaa tggagttgcg
      121 acattatcca gtgcttcatc atcgacacgt gggagtgccg cagtacaatt tctactgtgt
      181 ttattaggtg gtaagtcatg ggatgcatgt gtaaatagct acagaaatgc attggcacaa
      241 aatgcaggtg tctattcctt taatctgaca ttgtcataca acccgataac cacaacctgc
      301 aaaccggacg atttattaat tacgttagag agtattcccg tttcacaatt accagcca
//
LOCUS       DAPQZD010000440          346 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.453, whole genome shotgun sequence.
ACCESSION   DAPQZD010000440 DAPQZD010000000
VERSION     DAPQZD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.453"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            88..>346
                     /locus_tag="RO454_005141"
     CDS             88..>346
                     /locus_tag="RO454_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDY0936803.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYD"
BASE COUNT          108 a           68 c           65 g          105 t
ORIGIN      
        1 ctatcgacgt agcgttaaaa cttttaactc ttgtcaggat gctaaaaaca tcatgattca
       61 cagttaagtt aattctgaga gcatgaaatg aaaattacca tttccggtac tggctatgta
      121 ggcttgtcaa acgggcttct aatcgcacaa aatcatgagg ttgtggcatt agatatttta
      181 ccgtcacgcg ttgctatgct gaatgatcgg atatctccta ttgttgataa ggaaattcag
      241 cagtttttgc aatcagataa aatacacttt aatgccacat tagataaaaa tgaagcctac
      301 cgggatgctg attatgtcat catcgccact ccaaccgact atgatc
//
LOCUS       DAPQZD010000441          344 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.454, whole genome shotgun sequence.
ACCESSION   DAPQZD010000441 DAPQZD010000000
VERSION     DAPQZD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.454"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>344
                     /locus_tag="RO454_005142"
     CDS             <1..>344
                     /locus_tag="RO454_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDY0936804.1"
                     /translation="GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVE
                     LREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADT
                     GIDIDMHIDAASG"
BASE COUNT           80 a           97 c          103 g           64 t
ORIGIN      
        1 ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagccgcagg caaacccacg
       61 gataaaccaa acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac
      121 tgggatgtgg agttgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa
      181 cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc
      241 tacaccggta actatgagtt cccgcagccg ctgcacgatg cgctggataa attccaggcc
      301 gacaccggta tcgacatcga catgcacatc gacgccgcca gcgg
//
LOCUS       DAPQZD010000442          338 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.455, whole genome shotgun sequence.
ACCESSION   DAPQZD010000442 DAPQZD010000000
VERSION     DAPQZD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.455"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            5..>338
                     /locus_tag="RO454_005143"
     CDS             5..>338
                     /locus_tag="RO454_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936805.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGE
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTT"
BASE COUNT          103 a           69 c           78 g           88 t
ORIGIN      
        1 aattatgcaa ttacctgtaa agttattaat gagccttata tctctggtca gcgttattgc
       61 acgtgccggg aaatataaaa attacatccg ggatgaaata aaatactggc gatatacatc
      121 atacaagggg ggggaatttc cggaaggttt cactgatgag aaattttcca gcgccattta
      181 caacggaaga atatttacaa tgaaacgttt acataccctg atgttatttc tggcggttct
      241 gtttactggc tttaacgtgg aagcagcgag cgtgaaacaa gcgctcagct gcgacccaaa
      301 cgcccgggct gaacaacctg gagcgtgtcc aacaacgt
//
LOCUS       DAPQZD010000443          328 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.456, whole genome shotgun sequence.
ACCESSION   DAPQZD010000443 DAPQZD010000000
VERSION     DAPQZD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.456"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>328
                     /locus_tag="RO454_005144"
     CDS             <1..>328
                     /locus_tag="RO454_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HDY0936806.1"
                     /translation="WALIPGIQTQWMSAGSQYNPYLKISYQATPDLNLALRYRYDWKA
                     FRQTDLDGNRARNDQHQFDGYVTYKINDSWLFAWQTTVYTKVNNFKYGNHKKTATENA
                     FVLQYKM"
BASE COUNT          109 a           59 c           71 g           89 t
ORIGIN      
        1 atgggctcta atcccaggga ttcagacaca atggatgtca gcaggaagtc agtataaccc
       61 ttatctgaaa atttcgtatc aggcaacgcc agatcttaat ctggccttac gctatcgcta
      121 tgactggaaa gctttccgac agacggattt agatggcaac agggcgcgta atgatcagca
      181 tcagtttgat ggttatgtga catacaaaat taatgattca tggttgtttg catggcagac
      241 cacggtatat acgaaggtta ataatttcaa atatgggaat cataaaaaaa cagcaactga
      301 aaatgcattt gttctgcaat ataaaatg
//
LOCUS       DAPQZD010000444          325 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.457, whole genome shotgun sequence.
ACCESSION   DAPQZD010000444 DAPQZD010000000
VERSION     DAPQZD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.457"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="RO454_005145"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="RO454_005145"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDY0936807.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          111 a           55 c           83 g           76 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaaccg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaacagtcat taacgcacta gtaaaatgac cattcaggat
      241 gaggcggtaa gcttcatcct gatattgtta gttcagttgc ggtccagtta atcattctaa
      301 ataacaactg ctgaaattat acgaa
//
LOCUS       DAPQZD010000445          322 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.458, whole genome shotgun sequence.
ACCESSION   DAPQZD010000445 DAPQZD010000000
VERSION     DAPQZD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.458"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>322
                     /locus_tag="RO454_005146"
     CDS             <1..>322
                     /locus_tag="RO454_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0936808.1"
                     /translation="EALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRRI
                     AQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCP
                     NCGGS"
BASE COUNT           77 a           99 c           93 g           53 t
ORIGIN      
        1 gaagcgctga tccacgccct gcaggaaaag ctgagcaacc gggagcgcga aatagaccat
       61 ctgcaggcgc agctggataa actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc
      121 tcccgccgta tcgcacaaat ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg
      181 ctgactggta gggtgtatga cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt
      241 aagccgttcc ctgaatcact accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc
      301 tgcccgaact gcggcggttc ac
//
LOCUS       DAPQZD010000446          303 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.459, whole genome shotgun sequence.
ACCESSION   DAPQZD010000446 DAPQZD010000000
VERSION     DAPQZD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.459"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..282)
                     /gene="hcp"
                     /locus_tag="RO454_005147"
     CDS             complement(<1..282)
                     /gene="hcp"
                     /locus_tag="RO454_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDY0936809.1"
                     /translation="MGDIIYLRITGEQQGDISAGCGTQVSVGNRYQQGHEDEIFVFSF
                     QGGVSNTGFGINHQAIQFCKILDKSSPLLMNCINNNERCRFEFYFYRINK"
BASE COUNT           94 a           70 c           42 g           97 t
ORIGIN      
        1 tttattaata cgataaaaat aaaactcaaa acggcatcgt tcgttattat taatgcaatt
       61 catcagcagc ggtgagcttt tatcgagaat tttacaaaat tgtatcgctt gatgattaat
      121 gccaaatcct gtgttactta ctcctccctg aaaactaaaa acaaatattt catcctcatg
      181 tccctgttga taccgattcc cgacagagac ttgcgtcccg catcctgcag aaatatcccc
      241 ttgttgctcc cctgtaatgc gtaaatatat aatgtcaccc atatgattat acccatttca
      301 tag
//
LOCUS       DAPQZD010000447          302 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.460, whole genome shotgun sequence.
ACCESSION   DAPQZD010000447 DAPQZD010000000
VERSION     DAPQZD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.460"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..>302)
                     /locus_tag="RO454_005148"
     CDS             complement(<1..>302)
                     /locus_tag="RO454_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDY0936810.1"
                     /translation="IFMLFGTYKWFDFEVMALKPLISPTWLNILYIMFGVHGGSYFLG
                     IVETITFLALSIGFFRPAAGILGDIIVIVTGLVTLSLLPQLGKVDSFVIKDVLLLG"
BASE COUNT          104 a           70 c           59 g           69 t
ORIGIN      
        1 acctaacagc agcacatctt tgatgacaaa actgtcaact tttcccagtt gtggtaacag
       61 acttagtgtg actaaacctg taacaataac gataatatct cccagtatgc cagcagcagg
      121 cctgaagaag ccgatactca gtgctagaaa agtgatagtt tctactatgc cgaggaaata
      181 gctcccccca tgaacaccaa acataatgta caggatattc agccacgtgg gagaaatcag
      241 gggcttgagg gccatcactt caaaatcaaa ccatttatag gtcccgaaga gcataaatat
      301 ta
//
LOCUS       DAPQZD010000448          300 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.461, whole genome shotgun sequence.
ACCESSION   DAPQZD010000448 DAPQZD010000000
VERSION     DAPQZD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.461"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          103 a           43 c           72 g           82 t
ORIGIN      
        1 atgattttat actgaactaa agggggaaag aggtaacgaa aaaagacctc caccaggtac
       61 gaatctgagg cgggcagatg gaggtcgata tctacccgat taaatggata agtaaaagcc
      121 ccatcaggga ttgatttgtt cgagtctaat ttaaagtaat ttcaacgatt tggcagaact
      181 tcttagagtt aatgattttc aagttttcga ttaccaggaa aaaggctggg ggctgataaa
      241 atagtcatct gcattcctta aattgtgtaa gggtgaaagg ataaagttca atttaaaggc
//
LOCUS       DAPQZD010000449          298 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.462, whole genome shotgun sequence.
ACCESSION   DAPQZD010000449 DAPQZD010000000
VERSION     DAPQZD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.462"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>298
                     /locus_tag="RO454_005149"
     CDS             <1..>298
                     /locus_tag="RO454_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDY0936811.1"
                     /translation="FRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEA
                     VWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFL"
BASE COUNT           73 a           89 c           85 g           51 t
ORIGIN      
        1 ttccgctttg ccaccgacgc ccggctgaaa atcgaagttg tcgagtttta tgacgaccag
       61 agcggctacg agcgcgggct gacactgccg cttcgccatc cttcagggct gtttgatggt
      121 gaaacggaag cggtatgggg gctgaatacc gcgtacagcg tggtggaaaa aagcgtcacc
      181 actcgggact acaactaccg caccgccaca gcagagatga tgacagagca gcatgacgcc
      241 accgggggcg ataacaccac ctacggcgaa gcctaccact atgccgacaa cttcctgc
//
LOCUS       DAPQZD010000450          296 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.463, whole genome shotgun sequence.
ACCESSION   DAPQZD010000450 DAPQZD010000000
VERSION     DAPQZD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.463"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT          106 a           67 c           45 g           78 t
ORIGIN      
        1 aatttcggtt attggtgaac agagcggctc tctctgctcg ctaattccgc aagcctgaca
       61 aaaccaaagt gaataaaaat gcaattcaac cgaataaaag aaaacatcta tcactggtgc
      121 tttctgctgt caatgtacat tatctgtcat aaaaaatgct gaaaatgacc cggtcactac
      181 atacaccgaa ttctctacgc ttatagcgtt acctcacccc caaaccttca tgagtgaaaa
      241 taaaggcaag cacatatcca attgatttaa ttaaaaataa atcatatcgg catgta
//
LOCUS       DAPQZD010000451          293 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.464, whole genome shotgun sequence.
ACCESSION   DAPQZD010000451 DAPQZD010000000
VERSION     DAPQZD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.464"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT           95 a           52 c           41 g          105 t
ORIGIN      
        1 aaaaaccgat tgcccttaat atggttattg ttggatgttg taataattcc tcaaccaata
       61 tatcctctgc ttcatcacca agcaatctat aaagcctcaa acatgcagca taatggtact
      121 ctattgcatt atcatcttct gaagcttcaa aattagaatg ccgtattata tcaactaatc
      181 ctgttgatat tgttttaata tcactgtttg agagaagaaa ttcatttgtt tttattttgt
      241 catatatgtt tggtgatgat tataaaaaat ctaaagccca tagcccgaca agt
//
LOCUS       DAPQZD010000452          293 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.465, whole genome shotgun sequence.
ACCESSION   DAPQZD010000452 DAPQZD010000000
VERSION     DAPQZD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.465"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
BASE COUNT           82 a           52 c           66 g           93 t
ORIGIN      
        1 gggagcagcc actccctgca tcgactgtcc atagaatcct tagaggaggt tcctatgtgt
       61 gcgatggatc gtagagaaag agcactcatc agtcaattgc atttccctca tagtcttgga
      121 gaggctattt cgtttttaaa atatccagta tgtgtccgct tcgaggatgg ttcttttatt
      181 caggaaaata accgctttga aaaacttatc agatcgtcat ttaattcatg tgatgaatgg
      241 tttgatagtt taacgttgga atgtaaatta cagttatcca gagcagagat tga
//
LOCUS       DAPQZD010000453          293 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.denovo.466, whole genome shotgun sequence.
ACCESSION   DAPQZD010000453 DAPQZD010000000
VERSION     DAPQZD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.denovo.466"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..>293
                     /locus_tag="RO454_005150"
     CDS             <1..>293
                     /locus_tag="RO454_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDY0936812.1"
                     /translation="NPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT           63 a           77 c           87 g           66 t
ORIGIN      
        1 tgaacccatt caaaggccgg cattttcagc gtgacatcat tctgtgggcc gtacgctggt
       61 actgcaaata cggcatcagt taccgtgagc tgcaggagat gctggctgaa cgcggagtga
      121 atgtcgatca ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa atggaaaaac
      181 ggctgcgctg gtactggcgt aacccttccg atctttgccc gtggcacatg gatgaaacct
      241 acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc cgg
//
LOCUS       DAPQZD010000454        16055 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.109, whole genome shotgun sequence.
ACCESSION   DAPQZD010000454 DAPQZD010000000
VERSION     DAPQZD010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.109"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            29..1162
                     /locus_tag="RO454_005151"
     CDS             29..1162
                     /locus_tag="RO454_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDY0936813.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            1251..1784
                     /gene="blc"
                     /locus_tag="RO454_005152"
     CDS             1251..1784
                     /gene="blc"
                     /locus_tag="RO454_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDY0936814.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1781..2098)
                     /gene="sugE"
                     /locus_tag="RO454_005153"
     CDS             complement(1781..2098)
                     /gene="sugE"
                     /locus_tag="RO454_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDY0936815.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2274..2420)
                     /gene="ecnB"
                     /locus_tag="RO454_005154"
     CDS             complement(2274..2420)
                     /gene="ecnB"
                     /locus_tag="RO454_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDY0936816.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2531..2656)
                     /gene="ecnA"
                     /locus_tag="RO454_005155"
     CDS             complement(2531..2656)
                     /gene="ecnA"
                     /locus_tag="RO454_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDY0936817.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2708..3274)
                     /gene="efp"
                     /locus_tag="RO454_005156"
     CDS             complement(2708..3274)
                     /gene="efp"
                     /locus_tag="RO454_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDY0936818.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3316..4344
                     /gene="epmB"
                     /locus_tag="RO454_005157"
     CDS             3316..4344
                     /gene="epmB"
                     /locus_tag="RO454_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDY0936819.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4579..5448
                     /locus_tag="RO454_005158"
     CDS             4579..5448
                     /locus_tag="RO454_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDY0936820.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5498..5851)
                     /locus_tag="RO454_005159"
     CDS             complement(5498..5851)
                     /locus_tag="RO454_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDY0936821.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(5989..7635)
                     /gene="groL"
                     /locus_tag="RO454_005160"
     CDS             complement(5989..7635)
                     /gene="groL"
                     /locus_tag="RO454_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDY0936822.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7679..7972)
                     /locus_tag="RO454_005161"
     CDS             complement(7679..7972)
                     /locus_tag="RO454_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDY0936823.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8248..9504
                     /gene="yjeH"
                     /locus_tag="RO454_005162"
     CDS             8248..9504
                     /gene="yjeH"
                     /locus_tag="RO454_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDY0936824.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(9520..9996)
                     /gene="fxsA"
                     /locus_tag="RO454_005163"
     CDS             complement(9520..9996)
                     /gene="fxsA"
                     /locus_tag="RO454_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDY0936825.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            10333..11769
                     /gene="aspA"
                     /locus_tag="RO454_005164"
     CDS             10333..11769
                     /gene="aspA"
                     /locus_tag="RO454_005164"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDY0936826.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            11887..13188
                     /gene="dcuA"
                     /locus_tag="RO454_005165"
     CDS             11887..13188
                     /gene="dcuA"
                     /locus_tag="RO454_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDY0936827.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13304..13642
                     /gene="cutA"
                     /locus_tag="RO454_005166"
     CDS             13304..13642
                     /gene="cutA"
                     /locus_tag="RO454_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDY0936828.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            13618..15315
                     /gene="dsbD"
                     /locus_tag="RO454_005167"
     CDS             13618..15315
                     /gene="dsbD"
                     /locus_tag="RO454_005167"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDY0936829.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15352..15927
                     /locus_tag="RO454_005168"
     CDS             15352..15927
                     /locus_tag="RO454_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDY0936830.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         3943 a         4125 c         3942 g         4045 t
ORIGIN      
        1 gggccgtttt gtatggaaac cagaccctat gttcaaaacg acgctctgca ccttattaat
       61 taccgcctct tgctccacat ttgccgcccc tcaacaaatc aacgatattg tgcatcgcac
      121 aattaccccg cttatagagc aacaaaggat ccccggtatg gcggtagcgg taatttatca
      181 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
      241 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
      301 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc acaacaaaat actggcctga
      361 acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct acaccgctgg
      421 cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc tgcgcttcta
      481 tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat
      541 cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa
      601 aactcgtgtc ttccagccac tcaaactcaa tcatacgtgg attaatgtac cgtccgcaga
      661 agaaaagaac tacgcctggg gatatcgcga aggtaaggca gtacatgttt cgccaggggc
      721 gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
      781 aagcaattta aaaccccttg atatcaatga gaaaatactt caacaaggga tacaactggc
      841 acaatctcgc tactggcaaa ccggcgatat gtatcagggt ctgggctggg aaatgctgga
      901 ctggccggta aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
      961 acgccccgta aaaccgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
     1021 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
     1081 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagttaccg ccgcctggca
     1141 gattctcaac actctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctccgc
     1201 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
     1261 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacac
     1321 cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgctatctc ggcacctggt
     1381 atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat
     1441 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
     1501 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
     1561 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
     1621 aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca
     1681 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
     1741 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttaatgagtg ctgagtttca
     1801 gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact
     1861 caccgagcaa cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
     1921 cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc atactgacaa
     1981 tcatcgccgt cacggtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca
     2041 ggccaacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
     2101 caggctccgg aaaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca
     2161 aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact attacctatg
     2221 taactatcgc agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc
     2281 tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg
     2341 ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct
     2401 gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat
     2461 agcaaattaa taatagacaa catcgccaaa aatgacggaa atctgatgat ttttcggaag
     2521 agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gaggtgtttg atatcttcgc
     2581 cgaaacctcg agcggtgtta cagcccgtaa gcagcgtgct ggcaagcaaa acaagaacga
     2641 taaggcgttt catcatctct aacctgaata agtgatggtg cagcctgcag gccgcaccac
     2701 aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt
     2761 cgccgatttg tacaaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt
     2821 tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatccacg atttccagtt
     2881 caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact
     2941 ctgcctgatc cagcaaccat ttggcgttgt cgccaattgc ttttgcatca gcagacagct
     3001 gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca
     3061 ggttcatatc gacaacatca gcaccttcag cggaatcagt agatttgaag gttttttcta
     3121 cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg
     3181 gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac
     3241 cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg
     3301 tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg
     3361 ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata
     3421 gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg
     3481 ccccgctcat ttatcgatcg catggagaaa ggcaatccga acgatccgct tttgcgtcag
     3541 gtaattacct cgcaagatga gtttgttgtc gcgcccggat tctccaccga cccgctggaa
     3601 gaacagcaca gcgtagtgcc aggtttgttg cataaatacc acaaccgggc gcttttgctg
     3661 gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc
     3721 gaaaatcagg gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg
     3781 gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg
     3841 gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc
     3901 cgtctgccga ttgtgatccc ggcacgcatc acagatgcgc tggttgaacg cttttctcat
     3961 tctacgctgc aaatcttgct ggtgaatcac attaaccatg ccaatgaggt agatgaaaca
     4021 ttccgtcagg cgatggctaa gttgcgccgg gtaggcgtta ctttgctgaa ccagagcgtt
     4081 ctgttacgtg gtgtgaacga taacgcgcaa acgctggcaa acctgagtaa tgcgttgttc
     4141 gatgcgggtg tgatgccgta ttacctgcat gtgctcgata aagtacaggg cgcggcgcat
     4201 tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac actggtgtcg
     4261 ggatatctgg tgcctaaact ggcgcgagaa attggcggcg aacccagcaa aacaccgctg
     4321 gatctccagc tacgccagca gtaactgatt aaacagggaa gtttaatctt tgcccgttgc
     4381 ataaccttta attattcata tacattgctg ttattactat ttatataacc gacaccaata
     4441 attaaccttt cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac
     4501 tattactacc aagcccattt atctggctta taaactaaaa ttaattattt aattgagaaa
     4561 tggttaggga gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc
     4621 aagagtaata atttcattgc actggcgctg aaaattaaag aacctcgcaa taaagaatcg
     4681 ttgttcttta tgtctgccat ggaattgcgc gaccttctga ttgcgctgga aagtcgtctg
     4741 catcaaaagc ataaactgga tgctgctgca cgccttcagt atgaacaagc tcgcgataaa
     4801 gtcattaaga aaatggcaga aaatatccca gaaattctgg ttgatgagct taaaaatgcc
     4861 gatattaatc gtcgggttaa cacgctggaa cttaccgatg atcagggcga aaatcttacc
     4921 ttcgtattaa cactacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt
     4981 gaaatgctgg cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca
     5041 ttacgtatta cctcactgtt agatttcctg ccgttatatg atgtcgactg ccaggatgat
     5101 ggtaatctgg aatatgacac ctattcccaa ccggaatgga aacataactt gttcaaccat
     5161 tatctggcgg tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca
     5221 gtggtgaaaa cgcgtgaagc aacgccgggt aaagaagtgg aggcgatcac tcgccgaatg
     5281 cttgatttca gcccccgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgttcgc
     5341 accgtagcgg caaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat
     5401 cacttacgtg ttcaatccac cagcaaaact gcaccacagg cgaaataatc ttatctgttt
     5461 tactacgagg ctggaagtat ccagcctctg gatcacatca gttcgggcac ttataaacct
     5521 gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc
     5581 tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg
     5641 cgccgcgcat agaaccgcct tcttctccgt gttgcccgga aagccagttg ctttgcttac
     5701 ctgttgcagt accaatcagc tggcactctg cacctggttg ctcgtccaca atgcgtacac
     5761 tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac
     5821 cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg
     5881 ccactcgcgt cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc
     5941 agacatttct gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc
     6001 gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt
     6061 cggcaggtcg gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta
     6121 ctgcagagca gaacgggtta ctttggttgg atccaggata cccatatcga tcatgttgcc
     6181 gtattcttca gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc
     6241 aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc
     6301 acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag
     6361 tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc
     6421 agcacgggtc gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc
     6481 ggtagcagca ccaactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg
     6541 cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac
     6601 acggccctgg attgcagctt cttcacccac gccatcgatg atggtggtgg tgtctttgtt
     6661 gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc
     6721 gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt
     6781 acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt
     6841 gttaacaacc agagttgcca gtgcttcgcc ttcaacatct tcagcgatga tcagcagcgg
     6901 tttacctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt tggagatttt
     6961 cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt
     7021 gatgaagtaa ggagacaagt agccacggtc gaactgcata ccttcaacca cgtccagttc
     7081 gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat
     7141 cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcagagatgg taccaacctg
     7201 agcaatcgct ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc
     7261 ggtaacagct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc
     7321 tttcagaccc tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc
     7381 tgcagcgtca ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa
     7441 cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc
     7501 accgaaagat ttatccagaa ctacgttacg gcctttagga ccgagggtaa ctttcactgc
     7561 atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac
     7621 gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgcggatt
     7681 acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga
     7741 tcttctcaga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat
     7801 ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc
     7861 cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt
     7921 caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact
     7981 ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc
     8041 tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa gaaaaaaaat
     8101 tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgctatttct
     8161 tcatcaccgt cataagcaaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa
     8221 aatttgggcg caatcggaca tcaacccatg agtggactca aacaagaact ggggctggcc
     8281 cagggcatcg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc cgttcctgcg
     8341 ttagctgcgc tagtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta
     8401 gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggc
     8461 gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggttg
     8521 tttttatcgg tcattcccgt gggtttgcct gccgcgctac aaattgctgc cggattcggt
     8581 caggcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg
     8641 ctggtgtggt atatcggtac tcgcggtgcc agttccagtg ctaatctaca aacagttatt
     8701 gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat caaacctgcg
     8761 aatatcccct tccctgcgcc aggaaatatc gaacttaccg ggttatttgc tgcgttatca
     8821 gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa
     8881 aatccagagc gtgattttcc tcgtgctttg atgattggcc tgctgctggc aggattagtc
     8941 tattggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca aatggcggcg
     9001 gcagcatcgc ttcccaaaat tgtagtgcag ttgttcggtg taggagcgtt atggattgcc
     9061 tgcgtaattg gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc
     9121 cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct
     9181 cgccatatcc cgaataatgc cctcaatgcg gtgctcggct gctgcgtggt aagcacgttg
     9241 gtgattcatg ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt
     9301 attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga
     9361 ctactggcgg tggttggcgg gctattatgc gttctgttac tggcaatggt cggctggaaa
     9421 agtctctacg cgctgatcat gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg
     9481 ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt
     9541 atgatcaagg cggtcgcggt catcatcctt tcgctggtac tcaccatcaa aagtattacc
     9601 gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca aatgcggcat
     9661 caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac cgaggaagtc
     9721 ggtgaaaaag cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat
     9781 catctccgcc gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt
     9841 aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa ccagcacgag
     9901 ggtcagcaat acccccaata catgagcaac ctgaataaag attgaaatct caatatagac
     9961 ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg
    10021 gttaaacccg gctacctgaa tgggttgcga atcgcgttta gcttatattg tggtcattag
    10081 caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatccatt
    10141 tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccagg ttacggtaga
    10201 aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg
    10261 aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa
    10321 aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa
    10381 gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc
    10441 agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa
    10501 gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc
    10561 attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac
    10621 gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat
    10681 atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac
    10741 catgttaaca aatgtcagtc tactaacgac gcctacccga ccggtttccg tatcgcagtt
    10801 tactcttccc tgattaaact ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt
    10861 aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta
    10921 ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa
    10981 aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt
    11041 actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc
    11101 actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctccga ctgcggcgct
    11161 tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac
    11221 gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa cctgccggaa
    11281 ctgcaggcag gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt
    11341 aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca
    11401 ggtcagctgc agttgaacgt tatggagcca gtcattggcc aggccatgtt cgaatccgtt
    11461 cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct
    11521 aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac
    11581 ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag
    11641 agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt
    11701 ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc
    11761 gaacagtaat cgtacagggt agtacaaata aagaaggcac gtcagatggc gtgccttttt
    11821 tcttgtgagc agtaacttaa aaataacaat ttaatatcaa cttgttaaaa aacaaggaag
    11881 gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga
    11941 ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt
    12001 ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc
    12061 gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag
    12121 ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg
    12181 actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt
    12241 gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag
    12301 attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa
    12361 ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg
    12421 gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc tgacgatccg
    12481 atttatcgca agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa
    12541 atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt
    12601 atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccact gatgaacacc
    12661 accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa
    12721 gtcgataccg acaacattct caactccagc accttcaaag caggtatgag cgcctgtatt
    12781 tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc
    12841 aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc
    12901 tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct
    12961 ctggcactga acgtttctcc gctgaccgct gttgcttctt ttgctgcggt gtctggtctg
    13021 ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact
    13081 acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt
    13141 gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg acccatcgcg
    13201 gggcgttcac gccccgcttt ctttcccgcc gactaacatc ctttccccgt ccgttgtata
    13261 gtgacctctc tcttgcagtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag
    13321 ttcgaatacc acgtctgtcg tggtgctatg tacggcaccg gatgaagcga cagcccagga
    13381 tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc
    13441 tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt
    13501 aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata
    13561 tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg
    13621 gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta
    13681 ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc ttttgatttt
    13741 cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac
    13801 cgtaaacaga tccgcattac gccggaacac gcgaaaattg ctgacgtgca gctgccgcaa
    13861 ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg
    13921 cttcccgtaa ccatcaacca ggcaagtgcg ggagcaacgt taactgtcac ctaccagggc
    13981 tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg
    14041 gtcgccaaca acgaagcgtc acagcctgtg tctgttccgc agcaagagca gcccaccgcg
    14101 caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca
    14161 tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggcggtaa acagcggctt
    14221 tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc gctgacttac
    14281 acggcgctgg gtctggtggt tgccgccgca gggttacagt tccaggcggc gctacagcac
    14341 ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc
    14401 ttgtttactc tgcaactccc ctcttcgctg caaacacgtc tcacgctgat gagcaatcgc
    14461 caacagggcg gctcacctgg cggtgtgttt attatggggg cgattgccgg actgatctgt
    14521 tcaccatgca ccactgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac
    14581 atgtggctgg gcggcggcac gctttatctt tatgcgctgg gcatgggcct gccgctgatg
    14641 ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc
    14701 aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt
    14761 ggtgatatat ggggattacg cttgtggtcg gcgcttggtg tcgcattctt tggctgggcc
    14821 tttatcacca gcctacaggc caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg
    14881 gcagcggcat tggttagcgt acgcccactt caggattggg catttggtgc aacacatacc
    14941 gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag
    15001 gcgctcgttg aagccaaagg caaaccagtg atgttagatc tctatgccga ctggtgcgtc
    15061 gcctgtaaag agtttgagaa atacaccttc agcgactcgc aggtgcaaaa agcgttagca
    15121 gatacggtct tacttcaggc caacgtcact gccaacgacg cacaagatgt ggcgctgtta
    15181 aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag
    15241 catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc
    15301 gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt
    15361 gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg
    15421 ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca
    15481 gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg
    15541 cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagttgct ggcacgttat
    15601 caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctgtt tatcgctgcc
    15661 tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc
    15721 gcggcctacg atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg
    15781 gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt
    15841 agccaggcgg atgtcgacac cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc
    15901 tgccgtcagg gtggtgcact gacctgacag aaacacagaa aagaagcgat ttgccgcaat
    15961 cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt
    16021 aatgcgcccc gttgcccgga tagctcagtc ggtag
//
LOCUS       DAPQZD010000455        14343 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.120, whole genome shotgun sequence.
ACCESSION   DAPQZD010000455 DAPQZD010000000
VERSION     DAPQZD010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.120"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            96..1412
                     /gene="fliD"
                     /locus_tag="RO454_005169"
     CDS             96..1412
                     /gene="fliD"
                     /locus_tag="RO454_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDY0936831.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            1435..1827
                     /gene="fliS"
                     /locus_tag="RO454_005170"
     CDS             1435..1827
                     /gene="fliS"
                     /locus_tag="RO454_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDY0936832.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            1832..2143
                     /locus_tag="RO454_005171"
     CDS             1832..2143
                     /locus_tag="RO454_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDY0936833.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            2140..3201
                     /locus_tag="RO454_005172"
     CDS             2140..3201
                     /locus_tag="RO454_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDY0936834.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            3209..3676
                     /locus_tag="RO454_005173"
     CDS             3209..3676
                     /locus_tag="RO454_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDY0936835.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            3696..4412
                     /locus_tag="RO454_005174"
     CDS             3696..4412
                     /locus_tag="RO454_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDY0936836.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            4425..5288
                     /gene="motA"
                     /locus_tag="RO454_005175"
     CDS             4425..5288
                     /gene="motA"
                     /locus_tag="RO454_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDY0936837.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            5291..6214
                     /gene="lafU"
                     /locus_tag="RO454_005176"
     CDS             5291..6214
                     /gene="lafU"
                     /locus_tag="RO454_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDY0936838.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            6285..7340
                     /gene="dinB"
                     /locus_tag="RO454_005177"
     CDS             6285..7340
                     /gene="dinB"
                     /locus_tag="RO454_005177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDY0936839.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            7337..7789
                     /locus_tag="RO454_005178"
     CDS             7337..7789
                     /locus_tag="RO454_005178"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDY0936840.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            7979..9118
                     /locus_tag="RO454_005179"
     CDS             7979..9118
                     /locus_tag="RO454_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDY0936841.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            9115..9729
                     /locus_tag="RO454_005180"
     CDS             9115..9729
                     /locus_tag="RO454_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDY0936842.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9785..11242)
                     /gene="pepD"
                     /locus_tag="RO454_005181"
     CDS             complement(9785..11242)
                     /gene="pepD"
                     /locus_tag="RO454_005181"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDY0936843.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            11503..11961
                     /gene="gpt"
                     /locus_tag="RO454_005182"
     CDS             11503..11961
                     /gene="gpt"
                     /locus_tag="RO454_005182"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDY0936844.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            12053..13297
                     /gene="frsA"
                     /locus_tag="RO454_005183"
     CDS             12053..13297
                     /gene="frsA"
                     /locus_tag="RO454_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDY0936845.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            13355..13756
                     /gene="crl"
                     /locus_tag="RO454_005184"
     CDS             13355..13756
                     /gene="crl"
                     /locus_tag="RO454_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDY0936846.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(13795..>14343)
                     /locus_tag="RO454_005185"
     CDS             complement(13795..>14343)
                     /locus_tag="RO454_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDY0936847.1"
                     /translation="LQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDR
                     TNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAV
                     AQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQL
                     DSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         3561 a         3557 c         3905 g         3320 t
ORIGIN      
        1 tcatttccgc ctgtttttca atctattgat tattcacata aaaaatattg gcacgccttt
       61 tgccaataac ctgacattgt tatccggagt catcaatgat caacccaaga accattgcgc
      121 aagaaatcgc gtatgcggat gtcgctactc aggcgacgaa tttgcaggag aagcagagtg
      181 agctggatgc tgaaagcagc ggcctggact cgctcagctc ggcgttgagc gatttccaga
      241 gcgccgttga cgcgctgaac agcgataccg acggcccggt gacttttgcc gccaccagca
      301 ataatgactc ggcgaccgtt tccgccaatt cccaggcgca ggcgggaagc tactcgtttt
      361 tcgttgagca actggcgcag gggcagcaaa ccacgttcag tatgggcgac gacgcctttt
      421 ccgctaccgg tacttttgaa ctgacgatgg gcgacagcac tatggatatc gatctgtcgg
      481 cggcagatca gaacggtgac ggtgacggtt ttatcgacgc cagcgagctg gtgaatgcga
      541 ttaacgactc cgatgacaat ccgggcgtgt cggcggcact ggtaaaaacc gacggcacca
      601 ccacgattat gctcacctcc gatagcaccg gggcgcagag cgcgttttcg gtgagcgtaa
      661 cggggcatga tgccagcaac gatagcacca gtgcgcccgt tgccacggat gtttcctccg
      721 ctcaggacgc gattattcat ctcggtagcg ccagcggacc ggctatcacc aacagcagca
      781 acacgtttga tgatgtgatc cccggtgtca ccatgacttt taccgaagtc agcgattctg
      841 acagcgatct caccaccttt aacatcagtg aagattccag cgccagccag gagaaagtac
      901 agacctttgt cgatgcctat aacaccttga ttgatacggt tgattcgcta accacccacg
      961 gtgatgacag tagtagcgcc ggggtctttg cgggggatgc tggactcagc tcactggcaa
     1021 accagctcga tgacatcgcc catgccagct acaacggcgt gtcgattgtc gactatggca
     1081 tcacgctcga ttctcacggc cacttacaga ttgactccga ccagttcaac gacgcgatga
     1141 agagcgatcc cgacggcctg acctctattt tcgttggcga caacagcatg gtggcgcaga
     1201 tggacgacct gattaacacc tacacggact ccagcaacgg catcatcacc ctgcgccagc
     1261 agaacattga tgatcagatg agcaaaattc aggacgaagg cgatcagctt accgatacct
     1321 ataacgctaa ctacgaccgt tatctcgagg agtacaccaa tacgttggtt gaggtgtaca
     1381 ccatgaaagc cagtatggcg gcatttgcgt aattcagaag gaatatcctt aaagatgtac
     1441 caagtacagg acggttattc gcaatacaaa gagatcgatc tcgcggcgcg taccgcggcg
     1501 gcatcgcctc ttgaactggt gctggtgctg ttttctggcc tgatggacga gctggaacgc
     1561 gccagaagcc acattgaagg gcggcgtttc gagaagaaag cccagagcat caataaatgc
     1621 atcgacattc tcaatgcgct caccagttcg ctggaatttg aaaccggcgg cgagctggtg
     1681 gtcaaccttt cgcgcctcta tgaccactgt gtgtatcgct tgtatgaagc cagcggcgaa
     1741 ctgtcggcag agaaaatcga tgaagtgatg ctgatcttaa gcaacctgcg cgaaggctgg
     1801 gaagggcttt cgggcaagct gggttaagac gatggaaaaa caacgcaggc aactttttgc
     1861 actggtcgaa gcgatgaatg acgccctcga caagcaacgc tggcgacgcc ttccggcact
     1921 gtatcagcag ttgatgcgcc agtttcacga ttacgcggcg gcggaaaccg acgcggcgca
     1981 gttggatgcg gtgaaggcac aactgtacgg cgcgtttaat gccctgatta aacggcgcga
     2041 gcagcgcgca gaagtgttga aagcgcgtat ggaacagcat cagcggcatc aggaaggcgt
     2101 actggcctac tcaattgtta atttactttc ggagaagtca tgaatccggc attgcttgcc
     2161 acgctcggaa cgcttgcgga aaccgcttcg ttgaaggcgg atattttacc gccggtgagc
     2221 ggtgaaaacg cgcccgtgtt taccctgccg aaaatggtgg tcgcggcggt ggcggagcgc
     2281 gttcatagcg ctaaaaccag tcagcaacag gcgacgcgcc cgcaggagaa cgatccggtg
     2341 gcgatgcagg cgctaatggc gctgttactt ccgcaacctg ccgcgccgca tcaggacacg
     2401 ccgcagccgc gaaacgtcgc aacatcgccg gttatccagc aattgacgaa agcggtggtg
     2461 caaaacgcgc cgcaacgccc gacgcagcag caggaactca cgtcgttgcc gccgcagttg
     2521 caggaactga tcggtcagtt gccgcaggcg aaacccgaac agcaggccag actggcgact
     2581 tacgccagtg aagatttaca tgccattgcg ccgacgcagc cgcgcgtctc aacaccgcca
     2641 gctcgcccga aacctgaact aacccgtgtg accgcgcgcc cgcaggtcga gcgtaaaacg
     2701 gaaaaagtgc cggacagcga accggttatt gcgcgtgcgg tgttgcaggt taagacgcca
     2761 gagttggtca gcgatcatca ggagattgtc gccaaaccag ccacgctttc gatggacgaa
     2821 ctgggcgaaa aactgacgac gctgttgaaa gatcagatcc actttcagct caacaaacaa
     2881 cagcagatct ccaccattcg tctcgatcca ccgtcgcttg gcaagcttga gatcgccgta
     2941 cagttggata acggcaaact gacggtccat atcggtgcga atcaaagcga agtctgccgc
     3001 gcattgcagc agtttagcga cgatctccgc cagcatctga cggcgcaaaa ttttatggag
     3061 gtgaacgtac aggtttcctc cgaagggcag tcgcagcagc aacaacagtc gggccatcag
     3121 caggaagagg tgagtgccgc cttacagctt gatgatgcgc ctcaatttca acagaacgaa
     3181 tccgttttga tcaaagtgta aaggcaatat gaagaaaatc gtggtggcag gcgtggtcag
     3241 cgccgttctg gcgttggtgg tcggcgctgg agcaggatgg agtgtctggc atcattatgc
     3301 cggaaaagga aaaccgacta ccgccgcgca aacggaatcg gtggaagctc tggatgagag
     3361 caagagcgta tttgtgacgc tgccggaaac cattgtcact ttgcatgaca acaacggcgc
     3421 ggatcattat ttgtcggcgg aattagtgat ggtggtcgcc agtgacaaag aggcggaaaa
     3481 aataaagcat caggaaccgc tgtatcaaag tattgcggtt gaatgcctga cggaaatgaa
     3541 atttgaagat ttgcgcggca tgaaaatatc tgccatccgc aaactgattt ctgacgcgtt
     3601 aaagaaagat cttcagcgtc gcaaaatgac cgcaccgtat aaggatttgc tggtgaaaaa
     3661 agtggtcttc cagtaatcac caggcggata taaacatgct acaggatgta ttcgagtcag
     3721 aagaatttgc agccacgctg gtattaactc cgcagcagga gagccactac ttgcaggcct
     3781 atttaccgtt ggtgcgtaaa gtggtgcggc aacttgcccc gcagtgtaat tgcattattg
     3841 accgtcagga tatggaacaa acggcgttaa tggggctgtt gaacgccatt cgtcgctatg
     3901 ggctgccgga cgaaggtttt gcgggctatg ccgtgcaccg tattcgcggt gcgatcctgg
     3961 atgagctgcg cgctctcgac tggcgaccgc gccagctgcg acagaaatat catcagatga
     4021 aagacctgat tcgcgaaacg cgtaaaaaac tggggcacga accggagtgg caggagctgg
     4081 ctgaggaagg gatctcgcat gaagattatc tcgaatatca gcaactggaa ggggcggaaa
     4141 cgctcgccag cctggacgaa ctgctgaacg gcgaagggcc aggcattccg cttgctggcc
     4201 gggaactgga agatcagttt gtcactcagc aaatgttgca acacgcgctt tcacaattaa
     4261 gcgaaaaaga gcaactgatc ctctccatgt attaccagca tgagatgaat ctcaaagaga
     4321 ttgcgctggt attagggcta accgaggccc gaatttgtca actgaataaa cagatcgcgc
     4381 agaaaattcg tgattttgtc tatccgaatt aaagtgaaga aatcatgcaa aaaatattag
     4441 gtttactggt ggtgctcggc tgcgtatttg gcggttattt tgaagcgggc gggcaattag
     4501 tggctatctg gcaaccggcg gaaatgatca ttattttggg tgccggtttc ggggcgatga
     4561 ttattggtaa cccgaaacat gtgctgaaag agattgccca tcaaattaaa ggcgtgatca
     4621 gtaaaaaaca gctcgggccg gagtttcagc gtcagctatt aatgtgcctg tatgagttgc
     4681 tcgaaatggt gcaaaacggc ggtctgcgga tgttggatca gcatattgag cagccggaag
     4741 agagcaccat tttccagaaa tacccgctgg tgttaaccca gaagcggctg gtgaccttta
     4801 ttgctgataa ctttcgcctg atggcgatgg gtaaaatcga cgcccatgag ctggaaggca
     4861 ttctcgatca ggaactggac accgcggaag agtccctgtt aacgccgtcg cgctcgctgc
     4921 aacgtaccgc cgaagcgatg ccgggatttg gtatttgcgc ggcggtgctg ggcatcatca
     4981 tcaccatgca gtcgattgat ggatctatcg ctctgattgg cctgaaagtg gcggcggctc
     5041 tggtggggac gtttttaggc gtgtttatct gctactgcct gatggacccg ctagccaacg
     5101 ccatggaaca gcaggcgcgg gcggaacatt ctttgctgga atgcgtgcgc actgtgctgg
     5161 tggcgcaggc aggcggcaaa ccgacgctgc tggcggtgga cgccgggcgt aagctgctgc
     5221 atctggcgtc caaaccgacc ttcgccaacc tcgacgcgtg ggtgaatgcg atgctggagc
     5281 aagaataagc atgagaaaga cgggcaacca ccgcgaacgt ggggcaaaaa ccaccatcgt
     5341 ccggcggcag atcaaaaaga atcatgccgg gcatcacggc ggggcgtgga aggtggcgtt
     5401 cgccgacttt actttggcaa tgatggcgct atttatgacg ctgtggattg tcaacagcgt
     5461 cagtaaatcc gagcgtgaaa gcattatcgc cgcgctgcac gggcagtcga tttttaacgg
     5521 tggtgggttg tcgccgttaa ataaaatcag cccgtctcat ccgccaaaac ctgccaccgt
     5581 ggcggtgccg gaagagacgg agaaaaaagc gcgcgacgtc aacgagaaaa cggcgctgct
     5641 gaagaagaaa tccgccaccg agcttggcga gctggcaacc agtatcaata ccatcgcccg
     5701 tgatgcgcat atggaagcga atctggagat ggagattgtt ccccagggat tacgcgtgct
     5761 gattaaagac gaccagaacc gcaatatgtt cgaacgcggc agcgcgaaga ttatgccgtt
     5821 ctttaaaact ttgctggtgg agcttgcgcc agtgttcgac tcgctcgata acaaaattat
     5881 tattaccggg cataccgatg cgatggccta caaaaacaat atctacaaca actggaacct
     5941 ttcgggcgat cgcgcgcttt cagctcgtcg ggtgctggaa gaggccggaa tgccggaaga
     6001 taaagtgatg caggtaagtg caatggcgga ccagatgctg ctggatgcca aaaatccgca
     6061 aagcgcgggc aaccggcgca ttgagattat ggtgctgacc aaaagtgcgt ccgatacgct
     6121 gtatcaatac tttggtcagc atggggataa agtggtgcag ccgctggtgc aaaagctgga
     6181 taagcagcag gtgctttcgc agcgaacgcg ttaaatgctg aatctttacg catttctcaa
     6241 accctgaaat cactgtatac tttaccagta ttgagaggtg agcaatgcgt aaaatcattc
     6301 atgtggatat ggactgcttt ttcgcagcgg tggagatgcg cgacaatccc gccctgcgcg
     6361 atatccctat tgctattggc ggcagccgcg aacgacgggg ggtgatcagc accgccaatt
     6421 atcccgcgcg taaatttggc gtacgtagcg ctatgccgac agggatggcg ctcaaattat
     6481 gcccgcatct caccttgctt ccagggcgct ttgacgccta caaagaagcc tcaaatcata
     6541 tccgcgaaat tttctcgcgc tatacctcgc gcattgaacc gttatcactg gatgaagcct
     6601 atctcgatgt caccgatagc gtccattgcc acggttctgc gaccctcatc gcccaggaaa
     6661 tccgccagac gatcttcaac gagctgcaac tgacggcgtc tgcgggcgtg gcaccagtaa
     6721 agtttctcgc caaaatcgcc tccgacatga ataaacccaa cggccagttt gtgattacgc
     6781 cggcagaagt tccgacattt ttacaaacct taccgctggc aaaaattccc ggcgtcggca
     6841 aagtctcggc ggcaaaactg gaagcgatgg ggctgcggac ctgcggtgat gtgcaaaagt
     6901 gtgatctggt gatgctgctt aaacgctttg gcaaatttgg ccgcattttg tgggagcgta
     6961 gtcagggcat tgacgaacgc gacgttaaca gcgaaaggtt gcgaaaatcc gtcggtgtag
     7021 aacgtacgat ggcggaagat attcatcact ggtctgaatg tgaagcgatc atcgagcggc
     7081 tttaccctga actggagcgt cgtttggcaa aggtaaaacc tgatttgctg attgcccgcc
     7141 agggggtgaa attaaagttc gacgattttc agcaaaccac ccaggaacac gtctggccgc
     7201 ggctgaataa atctgatcta atcgccaccg cgcgtaaaac ctgggatgaa cgccgcggcg
     7261 ggcgcggtgt gcgactggtg gggctgcatg tgacgttgct tgacccgcaa atggaaagac
     7321 aactggtgct gggattatga ataacataca aataagaaac tatcagcctg gtgattttca
     7381 gcaactatgc gctattttcc ttcgagctgt tacgatgacg gcgagtcagc actattctcc
     7441 acagcaaatt gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa
     7501 atcacaagtg cgggttgcag ttattaatgc acaaccggtt ggttttatta cccgcattgg
     7561 acattatatc gatatgttat ttgttgaacc ggaatacacc cgacgcggag ttgccagcgc
     7621 tttgttaaaa ccattgatta agtctgaatc cgaacttacg gtggacgcca gcataaccgc
     7681 aaaacccttt tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg
     7741 gggaacgtgg tttactaatt tttatatgcg atataaaccg caacattaaa tccagcttgc
     7801 aatgaaaata acgcccgtct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg
     7861 taaatcatga caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt
     7921 tttcttgccc tggattcgtc tgccatttcc tgatttttat tcataaatgg aatggtacat
     7981 gggcaaatat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
     8041 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
     8101 cgttgtgggg atgccagatt tacatcccgg acgcggctac ccgattggcg cagcgttctt
     8161 ctctgttggt cgtttttacc cggcactggt cggcaatgat atcggctgcg gcatggcgct
     8221 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
     8281 agatctggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
     8341 acagcatccg tggcacaact cgcttggctc catcggtggc ggtaaccact tcgcagaact
     8401 gcaacagatt gatcaaatta tcgacgcgga actgtttgca cgggctggtc tggatgcgca
     8461 gcatctgcaa ctactggttc atagcggctc gaggggattg gggcagtcta ttttacagcg
     8521 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctacggta
     8581 tattgcggaa catgacgatg cgctggcgtt tgcgcgtatt aatcgccagc tgatcgcttt
     8641 gcgcataatg caacaagtta aggccaccgg aagtccggtt ctggatgtgg cgcataactt
     8701 tgttagcgcg tgccaaatcg gcgatcaaca gggctggttg catcgtaaag gcgccacgcc
     8761 ggatgacaac ggtctggtga ttattcccgg ttcacgcggc gattactcct ggctggttaa
     8821 acctgttgcg aacgaaaaaa cgctccactc gctggcgcat ggggcggggc gtaaatgggg
     8881 gcgcaccgag tgtaaagggc gtctggcagc gaaatacacg gcgacgcaac tctcgcggac
     8941 tgaacttggc agtcgggtaa tttgtcgcga taaacaactc atctttgaag aagcgccaca
     9001 agcctataaa tcggctgaaa gcgtggtgca atgtctggtg ctggctgggt taattattcc
     9061 tgtcgcgcga ctgcgcccgg tgctgacgct caaaaacagt ggagggaaaa aaggatgatc
     9121 ttgctacaac tctcctctgc tcaggggccg gaagaatgtt gtctcgcagt gaaaaaagca
     9181 ctggacaggc tgattaaaga agctacccga caggacgtcg cggtaacggt gctggaaaca
     9241 gaaacgggtc gctactctga cacactgcgt tcggcgctga tttctctgga tggcgataac
     9301 gcatgggctc taagcgaaag ctggtgcggc actattcagt ggatttgtcc gagtccgtat
     9361 cggcctcatc atgggcgcaa aaactggttt ctgggcattg ggcgttttac cgctgatgag
     9421 caggaacaat cggatgcaat ccgttatgag acgctgcgtt cgtcggggcc gggcggtcaa
     9481 catgtcaata aaaccgactc ggcggtacgc gccacgcatc tggcatccgg tattagcgtg
     9541 aaggttcagt cagagcgcag tcagcatgct aacaaacggc tggcgcgatt actgattgcc
     9601 tggaagctgg aacaacagca acaggaaaat agcgcggcgc tgaaatcgca gcggcgaatg
     9661 ttccatcacc agattgaacg tggcaacccg cgacgaacgt tcacagggat ggcttttatc
     9721 gaaggataat gaaggatgaa actgccggaa ggcgttaaac gccatccggc agcaaatcaa
     9781 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatggccaa
     9841 cgctttcgat atgaacttgc tcatccgggg agtgtggacc ggtgatggtt ggcccgatag
     9901 acaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     9961 taatctggat gttcggcgtc ttgttgaaca gacgctgata ggtttcacgt accagatgca
    10021 ttaccggaga gttagcgtcc ggctgccagc caggatatgc gccttttgct tcggttttcg
    10081 cacctgccag tttacccagc gaatccagca tgctcactac gtagtcttta ccgctgtcga
    10141 tcagagaacg gatcaggcag tgaatttcta cgttattgtc ggtcatggtc accacaccga
    10201 cgttcaggga ggtttcaacc acgcctttgg ctacatcgga attacggata actccgttcg
    10261 gggtggcgtt cagcagacga ataaaggtat cgcgagatgt cgccgttaat gcagctttgt
    10321 cgttcgctac ggagtccagc agcaatgcca gattcttctc tttttctgcc agttcgtttt
    10381 tcaggatctc ctgataggta tccaccagag atttcagggc gtcgacttta tcagctgcga
    10441 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
    10501 caataagacg caggtccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
    10561 tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta
    10621 aggttaaatt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
    10681 aggtgaagtc gatacccccc gcacatccca tgtagatttc accttcttct tcggagtcgg
    10741 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
    10801 cggcttcttc agtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
    10861 ccagaaccgc cagtgcggag gccataccaa tgccgttatc cgcacccagc gtggtgccgc
    10921 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttagtg aagtcatgca
    10981 cggtgtcgtt atttttttgc ggcaccatat cgaggtgggc ctgcaagacg accggtttac
    11041 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
    11101 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
    11161 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
    11221 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
    11281 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtttg
    11341 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
    11401 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
    11461 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
    11521 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
    11581 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg
    11641 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
    11701 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
    11761 attgatgacc tggtagatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
    11821 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgattatgtt
    11881 gttgatatcc cgcaaaatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc
    11941 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
    12001 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ctatgacaca
    12061 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
    12121 agtccgccgc tttagtcacg gcgcacaact gcctgtgcag tcggcccttg atggtaaaac
    12181 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
    12241 ccgtgaaatc ctcgaagtcc aggcgcgtat tgtgatgagc gatgccgaac gtaccgacga
    12301 tgatttatac gatacggtga ttggctaccg cggcggcaac tggatttatg agtgggctac
    12361 ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac tcagcggtcg
    12421 acactggctg catgcggcta cgttgtacaa cattgctgcc tatcctcatc tgaaaggaga
    12481 tgacctggcc gagcaagcgc aggctttgtc aaaccgcgcc tatgaagagg ccgctcagcg
    12541 tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg
    12601 ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct
    12661 ggatgcgatg cagacagact attacagcct gtatgaacgt tattttgcgc tgcgcggcat
    12721 tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca
    12781 ggactccagc ctgttgcatc agcacgtttt aaaggcgttg cctaacgtac cgtgggtgga
    12841 tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tacgtctggc
    12901 ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct
    12961 gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag
    13021 tcgtttgggg atgcatgatg cttctgacga agcgttgcgc gtggagctga atcgctattc
    13081 attaaaagtg caagggttgc tgggacgtcg ctgcccaacg ccaatgttat caggctactg
    13141 gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacgtcgt catctgctga
    13201 cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggccttca
    13261 ggaaatcaca gactggatcg aaaaacgttt gtgttaaaaa tttgctaaat tttaccaatt
    13321 tggtaaaaca gttgcatcac aacaggagat tgcaatgacg ttaccgagtg gacacccgaa
    13381 gagcagatta gtcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga
    13441 agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga
    13501 agtgcgtgag ttctggggct ggtggatgga actggaagcg caggaatccc gttttaccta
    13561 cagttaccag tttggtctgt tcgataaagc aggcgactgg acgagtgttc agataaaaga
    13621 tgccgaagtg gttgaacgac tggagcacac cctgcgtgaa tttcacgaga agctgcgtga
    13681 gctgctggcg acgctgaatc tgaagctgga accggcagat gattttcgtg atgaaccggt
    13741 gaagttaacg gcgtgaatga aatgtgccgg atgcatcaca tccggcaata tccattaaaa
    13801 ctgataggtc atgccaaccg cgacaatatc atcattatta atattcaatt tgttatcgct
    13861 atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga aataatacgt
    13921 agcaccaaca tcgacataat tgaccagatc ttcatcaccg atgccctcaa tatctttccc
    13981 tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac
    14041 cgcttcaaag ttctgcgtct tattggcaaa gccgccagag attggcgtca ttttacgtgt
    14101 ttcagaatag aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca
    14161 tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatcaga
    14221 gttggtatag gctccactga tggcgaaatc gctgccacca aagtcatatg tcaatgacgt
    14281 gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg
    14341 cag
//
LOCUS       DAPQZD010000456         5045 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.251, whole genome shotgun sequence.
ACCESSION   DAPQZD010000456 DAPQZD010000000
VERSION     DAPQZD010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.251"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..1050
                     /locus_tag="RO454_005186"
     CDS             <1..1050
                     /locus_tag="RO454_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDY0936848.1"
                     /translation="WLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQR
                     FRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYH
                     ESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPL
                     IGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIE
                     RTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGL
                     NLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRR
                     LEDGKFRPLRMPGKP"
     gene            1092..1334
                     /locus_tag="RO454_005187"
     CDS             1092..1334
                     /locus_tag="RO454_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDY0936849.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1366..2016)
                     /gene="tetR(A)"
                     /locus_tag="RO454_005188"
     CDS             complement(1366..2016)
                     /gene="tetR(A)"
                     /locus_tag="RO454_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDY0936850.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            2122..3321
                     /gene="tet(A)"
                     /locus_tag="RO454_005189"
     CDS             2122..3321
                     /gene="tet(A)"
                     /locus_tag="RO454_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDY0936851.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3353..4237)
                     /locus_tag="RO454_005190"
     CDS             complement(3353..4237)
                     /locus_tag="RO454_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDY0936852.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4375..4782)
                     /locus_tag="RO454_005191"
     CDS             complement(4375..4782)
                     /locus_tag="RO454_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDY0936853.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            4771..5045
                     /locus_tag="RO454_005192"
                     /pseudo
     CDS             4771..5045
                     /locus_tag="RO454_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          949 a         1619 c         1570 g          907 t
ORIGIN      
        1 tggctgcaag cctggcacat ccgcgacgaa acctattcgg cggccttggc cgagctggtc
       61 aaccaccagt atcgccacgc ctttgccgcc cactggggcg acggcacgac ctcatcctcc
      121 gatggccagc gcttccgcgc gggtggccgg ggcgagagca ccgggcacgt caacccgaag
      181 tacggtagcg agccgggacg gctgttctat acccatatct ccgaccagta cgcgccgttc
      241 agcacccgcg tggtgaatgt cggcgtccgc gattccacct atgtgctcga cggcctgctg
      301 taccacgagt ccgacctgcg gatcgaggag cactacaccg acacggccgg cttcaccgat
      361 cacgtctttg ccctgatgca cctgctaggc ttccgcttcg cgccgcgcat ccgcgacctc
      421 ggcgaaacca agctgtacgt gccgcagggc gtgcaagcct acccgacgtt gcgcccgctg
      481 atcggcggca ccctgaacat caagcacgtg cgtgcccact gggacgacat cctgcgcctg
      541 gccagctcga tcaagcaggg caccgtcacc gcctcgctga tgctgcgcaa gctcggcagc
      601 tacccgcgcc agaacggact ggccgtggcc ctgcgcgagc tgggccggat cgagcgcacg
      661 ctgttcatcc tggactggct gcaaagtgtt gaactgcgcc gccgcgtgca tgccggcctg
      721 aacaaaggtg aggcgcgcaa ctcgctggcc agggcggtgt tcttcaaccg ccttggggaa
      781 atcagggatc ggagcttcga gcagcagcgc taccgggcca gcggcctcaa cctggtgacg
      841 gcggctatcg tgctgtggaa cacggtgtac ctggaacgcg ccacccaggg gttggtcgag
      901 gccggcaagc cggtggacgg cgagctgctg caattcctgt cgccgctggg ctgggagcac
      961 atcaacctaa ccggcgatta cgtctggcgg cagagccgca gactggaaga cgggaagttt
     1021 cggcccttac ggatgcccgg aaaaccttag cgtacgattt tttccgaatt ctgcgggctc
     1081 ccctatctca tctgcgcaag gcagaacgtg aagacggccg cgagcgccag gcgcacgagg
     1141 ccggcgcgcg gacccgcgcc acggcccacg agcggacgcc gcagcaggag cgccagaagg
     1201 ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc
     1261 ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca
     1321 tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt
     1381 tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac
     1441 cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc
     1501 ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct
     1561 gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc
     1621 attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc
     1681 cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta
     1741 ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc
     1801 gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc
     1861 gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg
     1921 ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc
     1981 ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa
     2041 cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg
     2101 aggctggtcc ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact
     2161 gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc
     2221 gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg
     2281 ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg
     2341 ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg
     2401 cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg
     2461 gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc
     2521 ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg
     2581 atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat
     2641 ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc
     2701 cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc
     2761 gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg
     2821 gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc
     2881 gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg
     2941 ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg
     3001 cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt
     3061 ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag
     3121 gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc
     3181 ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca
     3241 ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca
     3301 gggcaacgag ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg
     3361 acttccggca agctatacgc gccctaggag tgcggttgga acgttggccc agccagatac
     3421 tcccgatcac gagcaggacg ccgatgattt gaagcgcact cagcgtctga tccaagaaca
     3481 accatcctag caacacggcg gtccccgggc tgagaaagcc cagtaaggaa acaactgtag
     3541 gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc
     3601 cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca
     3661 ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga
     3721 gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg
     3781 cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca
     3841 acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc
     3901 tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg
     3961 ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa
     4021 tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga
     4081 cgatcatcac gagcaataaa cccgccggca acgcccgcag catcgcgacc gtcatcggtg
     4141 agaagttcgg caggtattgg gtggtgacaa tgtaggtgct gccccaaatg gcaggtgcta
     4201 tcgctgtgaa caataaatcg ggcgtgcgta atgacatgcg attacaagac ctccgctacg
     4261 gcgatgggtt tttgagagtt gcaggcgccg cgatgaccgc accctgactt ggactggccc
     4321 tgcctcgcac caatccacct gcggctatgg ccgcgatcat gcgaggcccg aaaattacac
     4381 gacctgtaca cgtccgccga acccggaacg caacagagct tcgtgggcat cccgatccgg
     4441 gtcgtagcag cagtcctgga ccaaacgcac gtcgtagtcc gcatcactag cccaggcgac
     4501 gcttgaaaga acaacgccgg tggtgcttat cccggccatg acaagcgtgc ttacgccgcg
     4561 cgtccgaagg tcggcgtcga gcgccgtgcc ataaaagacg ctggctcgcg gacatgcata
     4621 gaaaaggtcg ccccgttcga tcgcaagccc ttcgacgggc agaccggtgc gaaatcgtcc
     4681 gctcgggaga tacggtgaga tttggcgatt cgtcgccggc ggcgcatgtt catactcttc
     4741 gccgagcgaa aagttgggga acagcacggg ccggccgcgc aaggcgtgct ggcagccgtg
     4801 cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga tgcacccacg
     4861 gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct cgaccggaaa
     4921 ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg cgacatctgg
     4981 gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc cgagaagttc
     5041 gccgc
//
LOCUS       DAPQZD010000457         4329 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.267, whole genome shotgun sequence.
ACCESSION   DAPQZD010000457 DAPQZD010000000
VERSION     DAPQZD010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.267"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            65..853
                     /locus_tag="RO454_005193"
     CDS             65..853
                     /locus_tag="RO454_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDY0936854.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1059..1406
                     /locus_tag="RO454_005194"
     CDS             1059..1406
                     /locus_tag="RO454_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDY0936855.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1400..2239
                     /gene="sul1"
                     /locus_tag="RO454_005195"
     CDS             1400..2239
                     /gene="sul1"
                     /locus_tag="RO454_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDY0936856.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2367..2570
                     /locus_tag="RO454_005196"
     CDS             2367..2570
                     /locus_tag="RO454_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDY0936857.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2726..3931
                     /gene="chrA"
                     /locus_tag="RO454_005197"
     CDS             2726..3931
                     /gene="chrA"
                     /locus_tag="RO454_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HDY0936858.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            3942..4247
                     /locus_tag="RO454_005198"
     CDS             3942..4247
                     /locus_tag="RO454_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDY0936859.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
BASE COUNT          830 a         1204 c         1269 g         1026 t
ORIGIN      
        1 ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg
       61 catcatgggt gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt
      121 gatcgagcgc catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga
      181 tggagggctg aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa
      241 cgattcgctc cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga
      301 tggcggaaca tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg
      361 gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc
      421 cggaacgttc gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag
      481 gcaacacagc cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa
      541 ggagcatttc tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg
      601 gaagggtgac gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac
      661 tggtctcatt gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga
      721 gcatcggccc ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct
      781 agcaatgcgc gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag
      841 aatcttgcgt tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg
      901 cctaactcgg cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac
      961 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     1021 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
     1081 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
     1141 ctaagcttgc cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt
     1201 ctctggttct gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg
     1261 tcgtcataat tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct
     1321 ttgtaggtat ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt
     1381 cgctgcggag gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc
     1441 cttcttcgat gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat
     1501 gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag
     1561 gcctgtatcg ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga
     1621 tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa
     1681 gcgcggcgtg ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga
     1741 tattgctgag gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc
     1801 cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga
     1861 ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc
     1921 ggggatggga tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct
     1981 tcaaaagctg aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt
     2041 cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga
     2101 acttcacgcg atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg
     2161 aagcgcaatc accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg
     2221 agggttagat catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt
     2281 tccgcgaagg tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg
     2341 cggttcatac cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg
     2401 cctgctcggg tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt
     2461 ccgccaaggg aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct
     2521 tcgtcctgag atccgagctc ctagggatcg cctcagaaaa cggaaaataa agcacgctaa
     2581 gccgtaagta agcgtgctcc tgtgaaagcc acagctaaaa ctgcgtagta cacatagagg
     2641 tatttttttc tagatttata tcgaatttcg ttatcgaatt tcgcttatta ttgatcaact
     2701 cgttgcagat catgaaggcg aaagtatgac gaataaccct accgatgaca gcagaccatg
     2761 gtcggtcttt cttatttttc tgcggcttgg attgacatct tttggcggcc ccattgcgca
     2821 cttgggctac ttccgcgccg aatttgtcac acggcggcgc tggctctccg aacggagcta
     2881 tgctgacttg gtcgcgcttt gtcagttctt gccagggcct gcaagcagcc aggtcggcat
     2941 agcggtagga ctgtctcggg ctggatacag cggggcgctg gctgcttggg ctggcttcac
     3001 gctgccgtct gccatagcct tgatcctttt tgcgctcggc atctccagct atggcgatta
     3061 cgtctcgcag ggcgcgttgc atggcttaaa agtggtggct gtggccgtgg tcgctcaagc
     3121 agtatggggc atggcgcgta acctatgcac ggatgggctg cgagtcacca tcatggcaat
     3181 tgctacctgc gtcgttttac ttgtgccgtc cgcgtgggga caggttggcg tgattgctat
     3241 cgcaggcatc gcaggccggt tattgttcaa gccagcgaaa gttgttgagc atgaccccct
     3301 acctatcacg gtcagtcacc gggccggcgt gctttggctc tcgctgttct ttgtcttgct
     3361 gattggcctg ccggtgttgg ccgaactgat gccaagtcaa accatggcaa tggtggattc
     3421 cttctatcgt gtcggatcac tggtgttcgg cggtggtcac gttgtgctgc cattactgca
     3481 agccgaagtg gtgccctccg gctgggtcaa caatgaatcc tttctcgcgg ggtacggggc
     3541 agctcaagcg gtgcccggcc ctttgttcac gttcgccgcg tttcttggtg cctcgatgaa
     3601 caccgccccg tcgggctgga tcggcggcat tgtgtgtctg ctggctatct tcgcgccctc
     3661 gttcttgctg gtcgtcggat caatgccatt ttgggagcgt ttgcgccgca atacaggcat
     3721 ccaagctgcg ctggccggga tcaatgccgc tgtagtcggc ttgctgctgg ccgcgctgta
     3781 tcagcctgta tggactagcg ccatctttca gccgcaagac ttcggcttgg cattagttgc
     3841 ccttgtcgca cttatgttct ggaagctccc gccgtggctg gttgtcctcg gtagcggtgc
     3901 agctgggtgg ctgttgagcg tcgctctgtg aggcacaaaa aatgactgac aaagacctct
     3961 acggcggttt gatccgcctg cacatccttc accatgcagc cgaggaacct gtctttgggc
     4021 tggggatcat cgaagagcta cgccgacacg gctacgagat gagcgctggc accgtgtacc
     4081 cgatgctgca cggcctggaa aaaaaaggct atctgacctc acgccacgaa cgcaccgggc
     4141 gacgtgaacg gcgtgtttat gacatcactg aacaaggcag aactgcactt gctgatgcga
     4201 aaacaaaggt caaggagctg ttcggagagt tggtagaagg tggttgagtt tttgcttcta
     4261 tgtcaggttg agctataccc taactggatt gtcattttca gaagacgact gcaccagttg
     4321 attgggcgt
//
LOCUS       DAPQZD010000458         4191 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.269, whole genome shotgun sequence.
ACCESSION   DAPQZD010000458 DAPQZD010000000
VERSION     DAPQZD010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.269"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            109..1014
                     /locus_tag="RO454_005199"
     CDS             109..1014
                     /locus_tag="RO454_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDY0936860.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /locus_tag="RO454_005200"
     CDS             1011..2249
                     /locus_tag="RO454_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDY0936861.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..2833
                     /locus_tag="RO454_005201"
     CDS             2249..2833
                     /locus_tag="RO454_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDY0936862.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3326..4090)
                     /locus_tag="RO454_005202"
     CDS             complement(3326..4090)
                     /locus_tag="RO454_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDY0936863.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          712 a         1306 c         1306 g          867 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtggtgg aggggatccg
     2641 caagcgactg cccccaggtg ctcctgcggc agctgagttg ctcctgcact cggtcatcgc
     2701 tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag ctagctgatc atgtgctggc
     2761 tcagatcgct gccatcctgt gtttaatgtt tcccgaacac gacgatttcc aactcctcca
     2821 ggcacatgcg taaacggagg tgtgcagagt ccctgcggca ggcgacgaac acgaccgtcg
     2881 tcgattagta ccggtacggt cggtggtatc gaagtcttga tcaccactca ggtctacggc
     2941 ttacaaatgg tgaccatccc gatacttgcg tcagagcacc gggccgattc tttgacagtg
     3001 aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg tcgagacgat actccgccaa
     3061 tgcgcccagc aaacaacctg gccatcgcag gtggtgggga gcggtgtggc ggatgagttg
     3121 gacaagttgg tgtagcagca cgagcacggc gagataacat cgcaggagtt cgacatgctc
     3181 aagagacagc tgattgcgaa tcgcgatgca gattcataac ccgattgcgg gttggcttca
     3241 ctccaccatc accgagcaga ctagcacggc gggctctgtt gcaaagattg gcggcagtca
     3301 gaggtaggct gtcgctctgc gccgatcagg cggctgctgc gaaatggtgg ttgagcatgc
     3361 ccatggcctc cgtcagcgcc gagggcccaa tgccaaaagc tctctccaca aggcgcacct
     3421 cgcccctgat gccgggctgc aggcaccagg ggcgagcctg tcctttgcgc agggctcgca
     3481 tgacttcgaa tcccttgatc gtggcatagg ccgtggggat cgatttgaaa ccgcgcaccg
     3541 gcttgatcag tatcttgagc tttccgtgat cggcctcgat cacgttattg agatacttca
     3601 cctgccggtg ggccgtctcc cggtccagct ttccttcgcg cttcaattcg gtgatcgctg
     3661 caccatagct cggcgctttg tcggtattga gcgtggcagg cttttcccag tgcttcaggc
     3721 ctcgcagggc cttgcccagg aaccgcttcg ctgccttggc gctgcgggtc ggcgacaggt
     3781 agaaatcgat cgtgtcgccc cgcttgtcga ctgcccggta caggtaggtc cacttgcccc
     3841 gcaccttgac gtaggtttca tccaggcgcc agctcggatc aaagccacgc cgccagaacc
     3901 agcgcagccg cttctccatc tccggggcgt agcactggac ccagcgatag atcgtcgtat
     3961 ggtcgaccga aatgccgcgt tccgccagca tttcctcaag gtcgcgatag ctgatcggat
     4021 agcgacaata ccagcgcacc gcccacagga tcacatcacc ctggaaatgg cgccacttga
     4081 aatccgtcat cgttccgtcc gtccaatctc cgccaagcat gctcaagctt cacgattttt
     4141 gcaacagagc ccacacgagt attgagcata gtcgagattg gtgcagatca c
//
LOCUS       DAPQZD010000459         4124 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.271, whole genome shotgun sequence.
ACCESSION   DAPQZD010000459 DAPQZD010000000
VERSION     DAPQZD010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.271"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..318
                     /locus_tag="RO454_005203"
     CDS             <1..318
                     /locus_tag="RO454_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDY0936864.1"
                     /translation="VIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLS
                     YNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGVYEK
                     CLV"
     gene            574..1449
                     /locus_tag="RO454_005204"
     CDS             574..1449
                     /locus_tag="RO454_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HDY0936865.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1496..1828)
                     /locus_tag="RO454_005205"
     CDS             complement(1496..1828)
                     /locus_tag="RO454_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDY0936866.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            1842..4085
                     /locus_tag="RO454_005206"
                     /pseudo
     CDS             1842..4085
                     /locus_tag="RO454_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
BASE COUNT         1049 a          991 c         1091 g          993 t
ORIGIN      
        1 gttatatact atgaaaagcg tggtaatgct gaaaactata tcaaagaagc caaatacgac
       61 atggcggtgg gtcatctctt gctaaagtca ttttgggcga atgaagccgt gtttcaaatg
      121 atgatgcttt catataacct atttttgttg ttcaagtttg attccttgga ctcttcagaa
      181 tacagacagc aaataaagac ctttcgtttg aagtatgtat ttcttgcagc aaaaataatc
      241 aaaaccgcaa gatatgtaat catgaagttg tcggaaaact atccgtacaa gggagtgtat
      301 gaaaaatgtc tggtataata agaatatcat caataaaatt gagtgttgct ctgtggataa
      361 cttgcagagt ttattaagta tcattgcagc aaagatgaaa tcaatgattt atcaaaaatg
      421 attgaaaggt ggttgtaaat aatgttacaa tgtgtgagaa gcagtctaaa ttcttcgtga
      481 aatagtgatt tttgaagcta ataaaaaaca cacgtggaat ttagggacta ttcatgttgt
      541 tgttatttcg tatcttccag aataaggaat cccatggtta aaaaatcact gcgccagttc
      601 acgctgatgg cgacggcaac cgtcacgctg ttgttaggaa gtgtgccgct gtatgcgcaa
      661 acggcggacg tacagcaaaa acttgccgaa ttagagcggc agtcgggagg cagactgggt
      721 gtggcattga ttaacacagc agataattcg caaatacttt atcgtgctga tgagcgcttt
      781 gcgatgtgca gcaccagtaa agtgatggcc gcggccgcgg tgctgaagaa aagtgaaagc
      841 gaaccgaatc tgttaaatca gcgagttgag atcaaaaaat ctgaccttgt taactataat
      901 ccgattgcgg aaaagcacgt caatgggacg atgtcactgg ctgagcttag cgcggccgcg
      961 ctacagtaca gcgataacgt ggcgatgaat aagctgattg ctcacgttgg cggcccggct
     1021 agcgtcaccg cgttcgcccg acagctggga gacgaaacgt tccgtctcga ccgtaccgag
     1081 ccgacgttaa acaccgccat tccgggcgat ccgcgtgata ccacttcacc tcgggcaatg
     1141 gcgcaaactc tgcggaatct gacgctgggt aaagcattgg gcgacagcca acgggcgcag
     1201 ctggtgacat ggatgaaagg caataccacc ggtgcagcga gcattcaggc tggactgcct
     1261 gcttcctggg ttgtggggga taaaaccggc agcggtggct atggcaccac caacgatatc
     1321 gcggtgatct ggccaaaaga tcgtgcgccg ctgattctgg tcacttactt cacccagcct
     1381 caacctaagg cagaaagccg tcgcgatgta ttagcgtcgg cggctaaaat cgtcaccgac
     1441 ggtttgtaat agcggaaacg gaatggggaa actcattccg tttttgttta tcgccttaga
     1501 cggcaaaagt gctgtcgccc acctgcgctt gcgcatacca ggccataagc tccgtggttc
     1561 ctggttctcc ttccgctgga gcccagtgcg catagtcatc ggcagccacg ggttgatagc
     1621 caccgtgttt tacttcaaaa attatgccac cggtatccag cgacagcacg gcatgccagg
     1681 ttcctgcggc catctccagc accgtacagg tttcccccaa tatcgcccga tgggtgacgg
     1741 taccccgatc gtcaaaattc agcaccacga aacgacccct taatggcaac agtagctcga
     1801 aggtgtgagg gtgtcggtgc gggcgcacgt aggtcccagg tcatattcct tccggcgtcc
     1861 ggcattttac cgccagacag ctcgggattc gtgatatcac cgttcttgca gaatacggtc
     1921 agagggaaaa tacccgccgt gagcatgcag cgctgatacg tcagcactat cagtatcgtg
     1981 aatttgcctg gccctggaca tttcgcctta cccgtctttt atatacccgg agctggataa
     2041 gcaacgaacg tcctggcctg cttttcgatc tggcgacagg gtggcttatg caacatcgta
     2101 ttattctccc cggagccact acgctgaccc ggttgatttc agaggtaagg gaaaaggcga
     2161 cgttgcgcct gtggaacaaa ctggcactga taccgtcagc cgaacagcgt tcacagctgg
     2221 agatgctgct ggggccaact gattgcagcc gcctgtcttt actggaatca ctgaaaaagg
     2281 gccctgtgac catcagtggt ccggcgttta atgaagcaat tgaacgctgg aaaactctga
     2341 acgattttgg cctgcatgct gaaaacctga gtacactccc ggctgtgcgc ctgaaaaatc
     2401 tcgcacgtta tgctggtatg acttcggtgt tcaatattgc caggatgtca ccgcagaaaa
     2461 ggatggcggt tctggttgcc tttgtccttg catgggaaac gctggcgctg gatgatgcat
     2521 tggacgttct ggacgccatg ctggccgtta tcatccgtga cgccagaaag attgggcaga
     2581 aaaaacggct ccgctcgctg aaggatctgg ataaatctgc attggcgctc gccagcgcat
     2641 gttcgtacct gctgaaagaa gaaacaccgg acgaatcgat tcgtgctgag gtgttcagct
     2701 acatcccaag gcaaaagctg gctgaaatca tcacgcttgt ccgtgaaatt gcccggccct
     2761 cagacgataa ttttcatgaa gaaatggtgg agcagtacgg gcgcgttcgt cgtttcctgc
     2821 cccatctgct gaataccgtt aaattttcat ccgcacctgc cggggttacc actctgaatg
     2881 cctgtgacta cctcagccgg gagttcagct cacggcggca gttttttgac gacgcaccaa
     2941 cggaaattat cagtcggtca tggaaacggc tggtgattaa caaggaaaaa catatcaccc
     3001 gcaggggata cacgctctgc tttctcagta aactgcagga tagtctgagg cggagggatg
     3061 tctacgttac cggcagtaac cggtggggag atcctcgtgc aagattacta cagggtgctg
     3121 actggcaggc aaaccggatt aaggtttatc gttctttggg gcacccgaca gacccgcagg
     3181 aagcaataaa atctctgggt catcagcttg atagtcgtta cagacaggtt gctgcacgtc
     3241 tttgcgaaaa tgaggctgtc gaactcgatg tttctggccc gaagccccgg ttgacaattt
     3301 ctcccctcgc cagtcttgat gagccggaca gtctgaaacg actgagcaaa atgatcagtg
     3361 atctactccc tccggtggat ttaacggagt tgctgctcga aattaacgcc cataccggat
     3421 ttgctgatga gtttttccat gctagtgaag ccagtgccag agttgatgat ctgcccgtca
     3481 gcatcagcgc cgtgctgatg gctgaagcct gcaatatcgg tctggaacca ctgatcagat
     3541 caaatgttcc tgcactgacc cgacaccggc tgaactggac aaaagcgaac tatctgcggg
     3601 ctgaaactat caccagcgct aatgccagac tggttgattt tcaggcaacg ctgccactgg
     3661 cacagatatg gggtggagga gaagtggcat ctgcagatgg aatgcgcttt gttacgccag
     3721 tcagaacaat caatgccgga ccgaaccgca aatactttgg taataacaga gggatcacct
     3781 ggtacaactt tgtgtccgat cagtattccg gctttcatgg catcgttata ccggggacgc
     3841 tgagggactc tatctttgtg ctggaaggtc ttctggaaca ggagaccggg ctgaatccaa
     3901 ccgaaattat gaccgataca gcaggtgcca gcgaacttgt ctttggcctt ttctggctgc
     3961 tgggatacca gttttctcca cgcctggctg atgccggtgc ttcggttttc tggcgaatgg
     4021 accatgatgc cgactatggc gtgctgaatg atattgccag agggcaatca gatccccgaa
     4081 aaatagggca ctgttgcaaa tagtcggtgg tgataaactt atca
//
LOCUS       DAPQZD010000460         2873 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.304, whole genome shotgun sequence.
ACCESSION   DAPQZD010000460 DAPQZD010000000
VERSION     DAPQZD010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.304"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            65..925
                     /locus_tag="RO454_005207"
     CDS             65..925
                     /locus_tag="RO454_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDY0936867.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1086..1583
                     /locus_tag="RO454_005208"
                     /pseudo
     CDS             1086..1583
                     /locus_tag="RO454_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
     gene            1601..1963
                     /gene="merD"
                     /locus_tag="RO454_005209"
     CDS             1601..1963
                     /gene="merD"
                     /locus_tag="RO454_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDY0936868.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            1960..2196
                     /gene="merE"
                     /locus_tag="RO454_005210"
     CDS             1960..2196
                     /gene="merE"
                     /locus_tag="RO454_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDY0936869.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            2232..>2873
                     /locus_tag="RO454_005211"
     CDS             2232..>2873
                     /locus_tag="RO454_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDY0936870.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGR"
BASE COUNT          589 a          820 c          861 g          603 t
ORIGIN      
        1 tatgtatccg ctcatgatac aataaccctg gtaaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt
      301 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgcagt gctgccataa ccatgagtga taacactgct gccaacttac ttctgacaac
      481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc cctacaagcg
     1081 tggaacacat ctacgccgca ggcgactgca ccgaccagcc gcagttcgtc tatgtggcgg
     1141 cagcggccgg cactcgcgcc gcgatcaaca tgaccggcgg tgacgcggcc ctgaacctga
     1201 ccgcgatgcc ggccgtggtg ttcaccgacc cgcaagtggc gaccgtaggc tacagcgagg
     1261 cggaagcgca ccatgacggc atcaaaactg atagtcgcac gctaacgctg gacaacgtgc
     1321 cgcgcgcgct cgccaacttc gacacgcgcg gcttcatcaa actggtggtt gaagaaggca
     1381 gcggacgact gatcggcgtg caggcagtgg ccccggaagc gggcgaactg atccagacgg
     1441 ccgcactggc gattcgcaac cggatgacgg tgcaggaact ggccgaccag ttgttcccct
     1501 acctgacgat ggtcgaaggg ttgaagctcg cggcgcagac cttcaacaag gatgtgaagc
     1561 agctttcctg ctgcgccggg tgaggacaag gaggtgtgcg atgagcgcct acacggtatc
     1621 gcaactggcc cataacgctg gggtgagcgt acatatcgtg cgcgactacc tggtgcgcgg
     1681 cttgttacgg ccggtggcct gcaccacggg cggctacggc gtgttcgacg atgcggcctt
     1741 gcaacggctg tgcttcgtgc gcgcggcctt cgaggcgggt atcggcctgg atgccctggc
     1801 gcggctgtgc cgtgcgctcg acgcagcgga cggcgcacaa gccgcagcgc agcttgccgt
     1861 gctgcgccag ttggtcgagc ggcggcgcgc ggcgttggcc catctggacg cgcaactggc
     1921 ctccatgcca gccgagcggg cgcacgagga ggcattgccg tgaacgcccc tgacaaactg
     1981 ccgcccgaga cgcgccaacc cgtttccggc tacctgtggg gtgcgctggc cgtgttgacc
     2041 tgcccctgcc atctgccgat tctcgccgcc gtgctggccg ggacgaccgc cggtgccttc
     2101 cttggcgagc attggggtgt tgccgcgctc gcgctgaccg gcttgttcgt tctggccgta
     2161 acgcggctgc tgcgcgcctt ccggggcgga tcatgacgag ttcgcagccc gccggatgga
     2221 cggcggccga gttggcgcag gcggcggcgc gcggacagct tgacctgcat taccagccgc
     2281 tggtcgatct gcgcgatcac cggatcgctg gcgcggaagc gttgatgcgc tggcggcatc
     2341 cgaggcttgg cctgttgccg cccggccagt tcctgccgct ggccgagtcg ttcggcctga
     2401 tgccggaaat aggcgcgtgg gtgctgggcg aggcctgtcg ccagatgcac aagtggcaag
     2461 gaccggcatg gcaaccgttc cgtcttgcca tcaatgtgtc cgccagccag gttgggccaa
     2521 cgttcgacga cgaggtaaag cgggtgctgg ccgatatggc cctgcccgcc gagcttctgg
     2581 agatcgaact gaccgaatcg gtcgcattcg gcaatccagc cctgttcgcc agtttcgacg
     2641 ccttgcgcgc catcggcgtg cgcttcgccg ccgacgactt cggcaccggc tattcctgcc
     2701 tgcaacatct gaaatgctgc cccatcacca cattgaaaat cgaccaatcc tttgtcgcca
     2761 ggctcccgga tgatgcccgt gaccaaacta tcgtgcgggc ggtgatccag ctcgcgcacg
     2821 ggctgggcat ggatgtcatt ttcagaagac gactgcacca gttgattggg cgt
//
LOCUS       DAPQZD010000461         2680 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.309, whole genome shotgun sequence.
ACCESSION   DAPQZD010000461 DAPQZD010000000
VERSION     DAPQZD010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.309"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            84..944
                     /gene="aac(3)-IIe"
                     /locus_tag="RO454_005212"
     CDS             84..944
                     /gene="aac(3)-IIe"
                     /locus_tag="RO454_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IIe"
                     /protein_id="HDY0936871.1"
                     /translation="MHTRKAITEAIRKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDKARRTWPPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRNGEVAWKTASEYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGATPIVPAHE
                     AAQRSCEPSG"
     gene            957..1499
                     /locus_tag="RO454_005213"
     CDS             957..1499
                     /locus_tag="RO454_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDY0936872.1"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVP
                     IPASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQL
                     LHIFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDS
                     GAHTSDELAAMVLDRLDQRT"
     gene            1591..>2680
                     /locus_tag="RO454_005214"
     CDS             join(1591..1849,1849..>2680)
                     /locus_tag="RO454_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDY0936873.1"
                     /translation="MTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDVHANQIKQ
                     WKDQLLEGATGVFGDETKAEPSGPTIDVKTLHAKIGELTLENGFFIRCARQGGIAGRK
                     EMIDREHKLSVVRQAKLLGFSRGSVYYLPRPVSDGDLALMRRIDELHLDYPFAGSRML
                     QGLLRGEGLETGRRHVATLMKKMGIKAIYRRPNTSKPAPGHIRKLAVTRPNQVWAMDL
                     TYIPMARGFVYLCAVVDWFSRKVLSWRLSITMEAAFCIEAVEEALARHGRPEIFNTDQ
                     GSQFTSIDFTAVLKRSQIAISMDGKGAWRDNVFVERLWRSIKYEEVYLHAYKTVSEAR
                     AGIARYLNFYNTRRPHWHCCKVSDEAAFCL"
BASE COUNT          590 a          737 c          814 g          539 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc tctcgatagg tatagtgttt
       61 tgcagtttag aggagatatc gcgatgcata cgcggaaggc aataacggag gcaattcgaa
      121 aattaggagt ccaaaccggt gacctgttga tggtgcatgc ctcacttaaa gcgattggtc
      181 cggtcgaagg aggagcggag acggtcgttg ccgcgttacg ctccgcggtt gggccgactg
      241 gcactgtgat gggatacgcg tcgtgggacc gatcacccta cgaggagact ctgaatggcg
      301 ctcggttgga tgacaaagcc cgccgtacct ggccgccgtt cgatcccgca acggccggga
      361 cttaccgtgg gttcggcctg ctgaatcaat ttctggttca agcccccggc gcgcggcgca
      421 gcgcgcaccc cgatgcatcg atggtcgcgg ttggtccgct agctgaaacg ctgacggagc
      481 ctcacgaact cggtcacgcc ttgggggaag ggtcgcccgt cgagcggttc gtccgccttg
      541 gcgggaaggc cctgctgttg ggtgcgccgc taaactccgt taccgcattg cactacgccg
      601 aggcggttgc ggatatcccc aacaaacgat gggtgacgta tgagatgccg atgcttggaa
      661 gaaacggtga agtcgcctgg aaaacggcat cagaatacga ttcaaacggc attctcgatt
      721 gctttgctat cgaaggaaag ccggatgcgg tcgaaactat agcaaatgct tacgtgaagc
      781 tcggtcgcca tcgagaaggt gtcgtgggct ttgctcagtg ctacctgttc gacgcgcagg
      841 acatcgtgac gttcggcgtc acctatcttg agaagcactt cggagccact ccgatcgtgc
      901 cagcacacga agccgcccag cgctcttgcg agccttccgg ttagaggccg tcgacaatga
      961 taatctggat caacggacct ttcggcgccg gaaagacgac tctcgctgag cggctgcgcg
     1021 atcggcgtcc gaaatcgctg atctttgacc ctgaggaaat cgggttcgtg gtgaaagaaa
     1081 cggtccccat accagcgagc ggagactatc aggatctccc cttgtggagg ggacttacga
     1141 tcgctgcggt cagcgagatt cgcaggaatt actcgcagga catcatcatc ccaatgacgc
     1201 tcgtgcaccc ggactatctg actgagatac tcgacgggtt aaggcagatc gacgatcagc
     1261 tgctgcacat ctttctgatg cttaacgagg acctattgcg tcaccggatc tcgaaccaga
     1321 ccatgcatcc tgacccgaat cggaatgcgg agattcgaga gtggcgatta gcgaatgtcg
     1381 cccgatgctt ggccgcaagg gaacggcttc cgtccacaac ccgtgttctc gatagtggtg
     1441 cacacaccag cgatgaactt gcagcgatgg tgctcgacag actcgatcag cgcacctgat
     1501 cgccttagag ctggctcggg aaatctgcac agcggggata agtggagttt ctgcctgact
     1561 tcggcttaga tgccgggaac aggagagacc atgacgagac gaccgcgccg gaaccatagc
     1621 ccggctttca aggcgaaggt ggcacttgcc gccatccgag gtgagcagac gctggtggag
     1681 ttgtcccagc agttcgatgt gcacgccaac cagatcaagc aatggaaaga ccagctcctt
     1741 gagggggcga caggtgtgtt cggcgatgaa acgaaagcgg agccgtcggg tccgaccatc
     1801 gatgtcaaaa cgctgcacgc gaaaatcggc gaactgacac tggagaacga ttttttatcc
     1861 ggtgcgctcg gcaaggcggg attgctgggc ggaaagaaat gatcgaccgc gagcacaagc
     1921 tatccgtcgt gcgccaggcg aagcttctcg gcttcagccg tggcagcgtc tactatctgc
     1981 ctcgtccggt gtctgacggc gatctggccc ttatgcgccg gattgacgaa ttgcatctcg
     2041 actacccctt tgccggaagt cggatgttgc aagggctctt gagaggagaa ggtctggaga
     2101 ccgggcggcg acacgtcgcc acgctgatga agaagatggg catcaaggcg atctaccgcc
     2161 gcccgaacac ctcgaaacca gcgccagggc acatacgaaa gctggcagtc acccggccca
     2221 atcaggtgtg ggcaatggac ctgacctaca tccccatggc gcggggattt gtctatctgt
     2281 gcgccgtcgt ggactggttc agccggaagg tcttgtcatg gcggttgtcg atcacgatgg
     2341 aagcagcctt ctgcatcgag gcggtggagg aggcacttgc ccgtcatggc aggccggaaa
     2401 tcttcaatac cgaccaggga tcgcagttca cctccatcga cttcaccgcc gtgctgaaga
     2461 ggtcacagat tgccatctcg atggatggca agggtgcgtg gcgagacaat gtcttcgtcg
     2521 agcggctctg gcgttcgatc aaatacgagg aagtctacct ccatgcctac aagactgtgt
     2581 ccgaggcacg cgctggcatc gcccgatatc tgaacttcta caacaccaga cgcccacatt
     2641 ggcactgttg caaagttagc gatgaggcag ccttttgtct
//
LOCUS       DAPQZD010000462         2480 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.316, whole genome shotgun sequence.
ACCESSION   DAPQZD010000462 DAPQZD010000000
VERSION     DAPQZD010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.316"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            complement(<1..207)
                     /locus_tag="RO454_005215"
     CDS             complement(<1..207)
                     /locus_tag="RO454_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDY0936874.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDGTVAKLAMRQPFV"
     gene            complement(333..683)
                     /locus_tag="RO454_005216"
     CDS             complement(333..683)
                     /locus_tag="RO454_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HDY0936875.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(886..1899)
                     /gene="intI1"
                     /locus_tag="RO454_005217"
     CDS             complement(886..1899)
                     /gene="intI1"
                     /locus_tag="RO454_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDY0936876.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2057..>2480
                     /gene="dfrA"
                     /locus_tag="RO454_005218"
     CDS             2057..>2480
                     /gene="dfrA"
                     /locus_tag="RO454_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HDY0936877.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQ"
BASE COUNT          575 a          687 c          683 g          535 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg tcgccggtgc gggcgaagct
       61 tatcagcgct tccagttgcg gacgcttgac atccttgccg gatgccttgt cgctaaaagc
      121 gcgatcaacc ttgacgcctt ccagttgccg ttccgggttc tggtcgaagg tgctgaccct
      181 gatataccca atgcgctgtc cagtcatgga attccctgca aaatgtcagg gaagactcta
      241 tgaccttcaa cgagatatgt caataaattc aaaattcaat cctatcctga cgcaatttac
      301 acatggcatc tgacatcagg ttagggtatg cctcaacctg acggcggcga atcacaagcg
      361 tccggtttga cgctggtttc ggtcgcagtg ctggcccgcg cctggaagcg ctgatagcac
      421 tccagcccgc agaaatgctc cacgtactcg gccccttccg gcgtgaaggc ggcatcgagc
      481 gggatttcct tgcagcacac gcagcaactg gtgctcggtt cattggcgtt catggtggtg
      541 ttcctccatt ggttgacgaa gccgacgaag gccgccgccg gcatcggcct ggcgaacagg
      601 aaaccctgca ccgtgtcgca acccgcctgc cgcaaccacg caaggcagtc gggtgtttcc
      661 acaccctcgg ctaccacctc cattgtcgtt ttcagaagac ggctgcactg aacgtcagaa
      721 gccgactgca ctatagcagc ggaggggttg gatccatcag gcaacgacgg gctgctgccg
      781 gccatcagcg gacgcaggga ggactttccg caaccggccg ttcgatgcgg caccgatggc
      841 cttcgcgcag gggtagtgaa tccgccagga ttgacttgcg ctgccctacc tctcactagt
      901 gaggggcggc agcgcatcaa gcggtgagcg cactccggca ccgccaactt tcagcacatg
      961 cgtgtaaatc atcgtcgtag agacgtcgga atggccgagc agatcctgca cggttcgaat
     1021 gtcgtaaccg ctgcggagca aggccgtcgc gaacgagtgg cggagggtgt gcggtgtggc
     1081 gggcttcgtg atgcctgctt gttctacggc acgtttgaag gcgcgctgaa aggtctggtc
     1141 atacatgtga tggcgacgca cgacaccgct ccgtggatcg gtcgaatgcg tgtgctgcgc
     1201 aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga tacttccgct caagggcgtc
     1261 gggaagcgca acgccgctgc ggccctcggc ctggtccttc agccaccatg cccgtgcacg
     1321 cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt
     1381 ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg
     1441 cagttgcaaa ccctcactga tccgcatgcc cgttccatac agaagctggg cgaacaaacg
     1501 atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg
     1561 caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat ccgtgcacag
     1621 caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac
     1681 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
     1741 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     1801 ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     1861 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1921 tgtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     1981 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagc
     2041 cattaaggga gttaaattga aaatatcatt gatttctgca gtgtcagaaa atggcgtaat
     2101 cggtagtggt cctgatatcc cgtggtcagt aaaaggtgag caactactct ttaaagcgct
     2161 cacatataat caatggctcc ttgtcggaag aaaaacattt gactctatgg gtgttcttcc
     2221 aaatcgcaaa tatgcagtag tgtcaaagaa cggaatttca agctcaaatg aaaacgtcct
     2281 agtttttcct tcaatagaaa atgctttgaa agagctatca aaagttacag atcatgtata
     2341 tgtctctggc gggggtcaaa tctataatag ccttattgaa aaagcagata taattcattt
     2401 gtctactgtt cacgttgaag tcgaaggtga tatcaaattc cctataatgc ctgagaattt
     2461 caatttggtt tttgaacagt
//
LOCUS       DAPQZD010000463          848 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.372, whole genome shotgun sequence.
ACCESSION   DAPQZD010000463 DAPQZD010000000
VERSION     DAPQZD010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.372"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            <1..787
                     /gene="blaOXA"
                     /locus_tag="RO454_005219"
     CDS             <1..787
                     /gene="blaOXA"
                     /locus_tag="RO454_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HDY0936878.1"
                     /translation="ANIIYSSASASTDISTVASPLFEGTEGCFLLYDASTNAEIAQFN
                     KAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQF
                     SVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQ
                     IQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEG
                     FIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          330 a          159 c          147 g          212 t
ORIGIN      
        1 tgcaaatatt atctacagca gcgccagtgc atcaacagat atctctactg ttgcatctcc
       61 attatttgaa ggaactgaag gttgtttttt actttacgat gcatccacaa acgctgaaat
      121 tgctcaattc aataaagcaa agtgtgcaac gcaaatggca ccagattcaa ctttcaagat
      181 cgcattatca cttatggcat ttgatgcgga aataatagat cagaaaacca tattcaaatg
      241 ggataaaacc cccaaaggaa tggagatctg gaacagcaat catacaccaa agacgtggat
      301 gcaattttct gttgtttggg tttcgcaaga aataacccaa aaaattggat taaataaaat
      361 caagaattat ctcaaagatt ttgattatgg aaatcaagac ttctctggag ataaagaaag
      421 aaacaacgga ttaacagaag catggctcga aagtagctta aaaatttcac cagaagaaca
      481 aattcaattc ctgcgtaaaa ttattaatca caatctccca gttaaaaact cagccataga
      541 aaacaccata gagaacatgt atctacaaga tctggataat agtacaaaac tgtatgggaa
      601 aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat
      661 tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc
      721 gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa
      781 tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa
      841 gcaagctt
//
LOCUS       DAPQZD010000464          831 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.376, whole genome shotgun sequence.
ACCESSION   DAPQZD010000464 DAPQZD010000000
VERSION     DAPQZD010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.376"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            131..685
                     /locus_tag="RO454_005220"
     CDS             131..685
                     /locus_tag="RO454_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoroquinolone-acetylating aminoglycoside
                     6'-N-acetyltransferase AAC(6')-Ib-cr5"
                     /protein_id="HDY0936879.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGRWEEETDPGVRGIDQL
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTV
                     TTPYGPAVYMVQTRQAFERTRSDA"
BASE COUNT          233 a          215 c          216 g          167 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgcct ttaagcgtgc ataataagcc
       61 ctacacaaat tgggagttag acatcatgag caacgcaaaa acaaagttag gcatcacaaa
      121 gtacagcatc gtgaccaaca gcaacgattc cgtcacactg cgcctcatga ctgagcatga
      181 ccttgcgatg ctctatgagt ggctaaatcg atctcatatc gtcgagtggt ggggcggaga
      241 agaagcacgc ccgacacttg ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca
      301 agagtccgtc actccataca ttgcaatgct gaatggagag ccgattgggt atgcccagtc
      361 gtacgttgct cttggaagcg gggacggacg gtgggaagaa gaaaccgatc caggagtacg
      421 cggaatagac cagttactgg cgaatgcatc acaactgggc aaaggcttgg gaaccaagct
      481 ggttcgagct ctggttgagt tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga
      541 cccgtcgccg agcaacttgc gagcgatccg atgctacgag aaagcggggt ttgagaggca
      601 aggtaccgta accaccccat atggtccagc cgtgtacatg gttcaaacac gccaggcatt
      661 cgagcgaaca cgcagtgatg cctaaccctt ccatcgaggg ggacgtccaa gggctggcgc
      721 ccttggccgc ccctcatgtc aaacgttggg cgaacccgga gcctcattaa ttgttagccg
      781 ttaaaattaa gccctttacc aaaccaatac ttattatgaa aaacacaata c
//
LOCUS       DAPQZD010000465          598 bp    DNA     linear   BCT 26-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain MS10584 isolate ARL02975
            SAMN15205494-rid18967693.guided.407, whole genome shotgun sequence.
ACCESSION   DAPQZD010000465 DAPQZD010000000
VERSION     DAPQZD010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15205494
            Sequence Read Archive: SRR12001666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001838409.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001838409.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/03/2023 21:13:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,134
            Genes (coding)                    :: 4,985
            CDSs (with protein)               :: 4,985
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 61 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 29 of 149
            Pseudo Genes (multiple problems)  :: 32 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15205494-rid18967693.guided.407"
                     /strain="MS10584"
                     /isolate="ARL02975"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="New Zealand"
                     /collection_date="2002"
     gene            117..>598
                     /locus_tag="RO454_005221"
     CDS             117..>598
                     /locus_tag="RO454_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDY0936880.1"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSAL
                     DAFQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDVGHCCKVSDEAAFC
                     L"
BASE COUNT          138 a          150 c          157 g          153 t
ORIGIN      
        1 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
       61 tcggcgcccc ttatttcaaa cgttagacgg caaagtcaca gaccgcggga tctcttatga
      121 ccaactactt tgatagcccc ttcaaaggca agctgctttc tgagcaagtg aagaacccca
      181 atatcaaagt tgggcggtac agctattact ctggctacta tcatgggcac tcattcgatg
      241 actgcgcacg gtatctgttt ccggaccgtg atgacgttga taagttgatc atcggtagtt
      301 tctgctctat cgggagtggg gcttccttta tcatggctgg caatcagggg catcggtacg
      361 actgggcatc atctttcccg ttcttttata tgcaggaaga acctgcattc tcaagcgcac
      421 tcgatgcctt ccaaaaagca ggtaatactg tcattggcaa tgacgtttgg atcggctctg
      481 aggcaatggt catgcccgga atcaagatcg ggcacggtgc ggtgataggc agccgctcgt
      541 tggtgacaaa agatgtgggg cactgttgca aagttagcga tgaggcagcc ttttgtct
//
